GSM9492465: HCC FFPE tumor targeted RNA-seq (AmpliSeq), sample H22-4344-Y, panel 2; Homo sapiens; RNA-Seq
Tumor regions were identified on hematoxylin and eosin-stained slides and manually dissected from 4-µm FFPE sections. Total RNA was extracted using the RNeasy FFPE Kit (QIAGEN) according to the manufacturer's instructions. RNA quantity and quality were assessed using a Qubit Fluorometer with the RNA HS Assay and a Bioanalyzer 2100. cDNA synthesis was performed using the AmpliSeq cDNA Synthesis for Illumina kit. Libraries were prepared using custom AmpliSeq for Illumina RNA panels targeting cancer progression– and immunity-related genes, together with AmpliSeq Library PLUS and CD Indexes Set A. Sequencing was performed on an Illumina MiSeq system using 2 × 150 bp paired-end reads.