Deep whole-genome sequencing of outbred Sprague-Dawley rat individual 11989
High molecular weight genomic DNA was isolated from outbred Sprague-Dawley rat spleen samples at the University of Michigan using the MagAttract HMW DNA Kit and transferred to the University of California San Diego for sequencing. Deep whole-genome sequencing libraries were prepared using KAPA HyperPrep and sequenced as paired-end 150 bp reads on an Illumina NovaSeq 6000 to approximately 19x as a truth set for genotype accuracy assessment.