GSM9560413: Irreversible pulpitis tissue sample 1; Homo sapiens; OTHER
Samples were assessed for RNA quality, and all FFPE samples had a DV200 score above the minimum threshold of ≥30%. Spatial transcriptomic libraries were generated with the Visium v2 Human Transcriptome gene expression kit (10x Genomics: 1000443). Five-micron FFPE sections were placed on Superfrost Plus slides (FisherScientific) and processed through the 10x Visium v2 for FFPE protocol according to the manufacturer's instructions (CG000518, CG000520, CG000495). H&E images were taken at 20x magnification using a Leica Aperio CS2 slide scanner (Leica Biosystems). Library QC was completed with an Agilent TapeStation 4150. Libraries were normalized and pooled to 2nM prior to loading on an Illumina NextSeq 2000, using a P4 100 flow cell with a target of a minimum of 120 million reads per sample for transcriptomic libraries.