GSM9480799: Patient 59 intrahepathic cholangiocarcinoma; Homo sapiens; RNA-Seq
Total RNA were extracted using Maxwell® RSC FFPE RNA kit (Promega), following the manufacturer's instructions. Concentrations were measured using Qubit RNA HS assays (Thermo Fisher Scientific). RNA sequencing was performed using the RNA Prep with Enrichment, (L) Tagmentation in combination with the RNA Pan-cancer panel (1,362 genes, Illumina) according to the manufacturer's recommendations. Libraries were sequenced on a NextSeq 500 instrument (2×150 cycles, Illumina).