16S rRNA gene V4V5 amplicon sequencing of Poas Volcano sample: Pb_1_PoasH2O_ex1
The V4-V5 region of the 16S ribosomal RNA gene was amplified using 515f/806r primers (Caporaso et al. 2011). Each primer combination had a unique barcode corresponding to a unique sample. Barcoded amplicons were normalized and pooled into a single library which was sequenced on a 2x250 Illumina MiSeq run. Sequences were subsequently demultiplexed by barcode into individual files corresponding to each sample.