GSM7976266: Female-Male, CD8+ T cells, day40, Armstrong; Mus musculus; RNA-Seq
Murine splenocytes were harvested at 4, 7, 28, and 40 days after virus infection and processed into single cell suspensions of CD8 T lymphocytes. Following CD8 T cell isolation by magnetic bead based enrichment, naive and activated CD8 T lymphocytes harvested at different times post-infection were FACS sorted on a BD Aria III.  The purity of sorted cell populations was confirmed on the BD Aria III prior to downstream scRNA-seq cDNA library generation. 10x Genomics Next GEM Single Cell 3' v3.1 protocol, single-index