GSM8965591: D3TA-1; Mus musculus; RNA-Seq
RNA was isolated using the RNeasy Plus kit following the manufacturer's instructions. mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The end repair, A-tailing, adapter ligation, size selection, amplification, and purification were performed.