GSM9283598: WildTypeB1; Mus musculus; RNA-Seq
Libraries were prepared from formalin fixed brains. Brainswere placed in Formalin-fixed paraffin-embedded (FFPE) blocks. The tissues were then process according to the manufacturer indications. 5µm microtome sections were placed on Visium Spatial Gene Expression slides (10x Genomics, #2000233)  Visium Mouse Transcriptome Probe Kit, Small PN-1000365 and preparation of cDNA libraries. cDNA libraries were prepared according to the manufacturer's protocol (https://www.10xgenomics.com/support/spatial-gene-expression-ffpe/documentation/steps/library-construction). Visium Mouse Transcriptome Probe Kit, Small PN-1000365 (Spatial 3' v1).Paired-end, dual index configuration with a read length of 28 bp for read 1 (spatial barcode and UMI), 10 bp index read (i7 index), 10 bp index read (i5 index), and 50 bp for read 2 (RNA read)