GSM8974497: Uveal melanoma in a singular eye from three 4-6 month old casper (mitfa -/-, mpv17 -/-) zebrafish; Danio rerio; RNA-Seq
Zebrafish eyes or skin were harvested with and without tumors from 4-6 month old AB wildtype and casper (mitfa -/-, mpv17 -/-) zebrafish. Tissues were made into single cell suspension using collagenase/hyaluronidase and trypsin and prepared according to 10X Genomics cell preparation guidelines. Approximately, 10,000 cells were loaded per channel to generate single-cell gel beads-in-emulsion (GEMs). Single-cell RNA-Seq libraries were prepared with Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1 (#1000128, 10X Genomics), Chromium Next GEM Chip G Single Cell Kit (#1000127, 10X Genomics), and Chromium i7 Multiplex Kit (#120262, 10X Genomics). Libraries were sequenced on an Illumina HiSeq 6000 with 150 bp paired-end reads in the genomics core at University of Iowa (Iowa City, IA).