GSM8942847: MCD, Splenic CD8+ T cells, ATAC-seq, rep 1; Mus musculus; ATAC-seq
splenic CD8+ T cells were washed by TW buffer for two times and lysed in lysis buffer (RS buffer, 10% NP 40, 10% Tween 20, 1% Digitonin) on ice for 5 min. ATAC libraries were prepared by Hyperactive ATAC-Seq Library Prep Kit for Illumina (TD711, Vazyme, Nanjing, China). Briefly, approximately 1×105 splenic CD8+ T cells were washed by TW buffer for two times and lysed in lysis buffer (RS buffer, 10% NP 40, 10% Tween 20, 1% Digitonin) on ice for 5 min. The samples were then mixed with fragmented buffer at 37 ℃ for 30 min and stopped by stop buffer for 5 min at room temperature. After this, ATAC DNA Extract Beads were applied to extract DNA and the extracted DNA was amplified with TruePrep Index Kit V2 for Illumina (TD202, Vazyme). The PCR products were further size selected by ATAC DNA Clean Beads. The sequencing of prepared libraries was performed by Novaseq X Plus platform at NovoGene (Beijing, China).