CRISPR RB1/ AMSC, osteogenic differentiation Day 0, replicate 2
This study profiles gene expression changes during osteogenic differentiation of CRISPR-edited RB1/ adipose-derived mesenchymal stem cells (AMSCs). PolyA-selected RNA-seq libraries were prepared and sequenced at four time points (days 0, 7, 14, and 21) to investigate the transcriptional consequences of complete RB1 loss during lineage commitment.