GSM9618183: PUER cells, IL3, 200 nM OHT, 48h, IgG; Mus musculus; OTHER
The CUT&RUN assay was performed using the CUTANA CUT&RUN kit (Epycypher, #14-1048), following the manufacturer's instructions. Briefly, 100,000 undifferentiated and 48h IL3-OHT PUER cells were harvested, immobilized to activated Concanavalin A beads and permeabilized with digitonin. 0.5μg of α-PU.1 (Santa Cruz, #sc-390405) primary and a 1:200 dilution of Rabbit anti-Mouse IgG (Cytek, #70-8076-M002) secondary were added to the samples before treatment with pAG-MNase for 2 hours. The released DNA fragments were purified and libraries prepared. Libraries were prepared using the CUTANA CUT&RUN Library Prep Kit (Epicypher, #14-1001) according to manufacturer's instructions.