COI amplicons: reef biofilm month 6
Genomic DNA was extracted using DNeasy PowerBiofilm Kits following the manufacturers protocol. The cytochrome c oxidase I (COI) gene from mitochondrial DNA was amplified with PCR using the primers mlCOIintF-XT (5-GGW ACW RGW TGR ACW ITI TAY CCY CC-3, Wangensteen et al. 2018) and jgHCO2198 (5-TAI ACY TCI GGR TGI CCR AAR AAY CA-3, Geller et al. 2013). PCR was run in 20-L reactions in DEPC-treated water using an AmpliTaq Gold PCR Kit according to published protocols (Wangensteen et al. 2018). COI amplicons were cleaned using a QIAquick PCR Purification Kit and sent to the Integrated Microbiome Resource at Dalhousie University for amplicon sequencing with an Illumina MiSeq 600v3 Reagent Kit and a 2x300 paired end configuration.