GSM8965359: WJ-MSCs Normoxia; Homo sapiens; miRNA-Seq
Trizol chloroform extraction was performed and total RNA was isolated The small RNA sequencing libraries were prepared from the received total RNA samples using illumina NEBNext® Multiplex Small RNA Library Prep Kit for Illumina (NEB) as per the manufacturer's instruction. Briefly, approximately 1ug of RNA was processed for 3' adapter ligation followed by 5' adaptor ligation. The adaptor ligated RNA samples were then converted to cDNA using reverse transcriptase followed by PCR amplification using common primer and indexed primer. The enriched library was then purified using Spin column followed by AMPureXP bead-based size selection.