GSM8988605: Bcl11b-KO, Cxxc1-ChIP; Mus musculus; ChIP-Seq
Freshly isolated thymocytes cross-linked with 1 mg/ml disuccinimidyl glutarate and 1% formaldehyde. Nuclei were isolated from the cells and lysed in Lysis Buffer by sonication. The lysate was incubated with rabbit anti-Cxxc1 antibody (abcam, EPR19199) pre-bound to Dynabeads® protein G overnight at 4 oC. Chromatin DNA was reverse-crosslinked and eluted. Samples were treated with RNase A and Proteinase K, and precipitated chromatin DNA was purified by use of ChIP DNA Clean & ConcentratorTM (ZYMO Research, CA). Library preparation were done using NEBNext Ultra II DNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina (New England BioLabs). Sequencing was performed by Immugen with HiSeq X Ten.