RNA-Seq of Mesocricetus auratus: male kidney
Total RNA was isolated from hamster tissues using FastGene RNA Basic Kit (Nippon Genetics, Tokyo, Japan). RNA purity, quantity, and integrity were assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Liver samples from all six individuals were analyzed separately, whereas small intestine and kidney samples were pooled from the six individuals and analyzed together. Strand-specific libraries were prepared using Illumina TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA). Sequencing was performed on an Illumina NovaSeq X Plus platform, generating 6 Gb of data in 150 bp paired-end mode.