GSM9283690: ddm1 rice panicle; Oryza sativa; RNA-Seq
RNA extraction was conducted using TRIzol reagent (Invitrogen). Ethanol precipitation was performed overnight at -20ºC to maximize sRNA yield. Small RNA-seq libraries were constructed using the VAHTS Small RNA Library Prep Kit for Illumina v2 (Vazyme, China), followed by purification with VAHTS DNA Clean Beads (Vazyme). cDNA libraries were quantified using NanoDrop spectrophotometer, and sequenced on an Illumina Hiseq X Ten platform (Illumina Inc., USA).