GSM8943414: CD8+T cells, RPMI 1640 methionine (-) medium; Mus musculus; RNA-Seq
Total RNA was extracted using TRIzol reagent and conserved in RNase-free tubes by lipid nitrogen quick freezing. The total RNA (10-1000 ng) was applied to construct cDNA libraries using Whole RNA-seq Lib Prep kit for illumina (ABclonal, RK20303) or mRNA-seq Lib Prep Kit for illumina (ABclonal, RK20302) following the manufacturer's instructions.