felis catus faecal metagenome
NucleoSpin Soil kits (Macherey-Nagel, Dren, Germany) were used to extract DNA from faecal samples according to the manufacturers instructions, with the addition of a bead beating step using a Mini-Beadbeater-96 for four minutes (BioSpec Products, Bartlesville, OK, USA). Analysis of the faecal metagenome was determined using dual lane, paired-end (PE150) Illumina HiSeq shotgun sequencing (Custom Science, New Zealand).