GSM9120588: iPSCod tubuloids FAF 7d; Homo sapiens; RNA-Seq
After 7 days of differentiation, iPSCod tubuloids were recovered by addition of 1 mg/mL dispase II (Thermofisher Scientific) to the medium and incubation for 30 min at 37ºC. Tubuloids were subsequently dissociated by treatment with Accutase (Thermofisher Scientific) and mechanical shearing using a flame-polished Pasteur pipette. After washing with PBS, the cell suspension was fixed by adding frozen methanol dropwise. The sample was thereafter stored at -80 ºC until further processing. Libraries were prepared using 10x Genomics Chromium 3' Gene Expression and sequenced on a NovaSeq 6000 (Illumina).