GSM8121276: Eu-eMSCs-Exos-3; Homo sapiens; miRNA-Seq
When eMSCs reached approximately 70% confluence, the medium was replaced with DMEM/F12 containing 10% exosome depleted FBS (Exo-FBS) (System Biosciences, USA) for 48 h. The cell culture supernatants of eMSCs were collected for microRNA (miRNA) sequencing (miRNA-seq). Exosomes derived from eMSCs (eMSCs-Exos) were isolated using the exoEasy Maxi Kit (Qiagen, USA), and their miRNA content was extracted using the miRNeasy Micro Kit (Qiagen, USA). 4μg total RNA was used for construction of sequencing libraries. MicroRNA libraries for miRNA-seq were prepared using the QIAseq miRNA Library Kit (Qiagen, Germany) following the manufacturer's standard protocol.