RAD21_RASH-1_CR_Rep1
CUT&RUN was performed using the CUTANA ChIC/CUT&RUN Kit (Epicypher) on RASH-1 cells. Nuclei were extracted, bound to concanavalin A-coated beads, and incubated with specific antibodies overnight. MNase digestion was performed, and DNA was purified using SPRIselect beads. Libraries were prepared with the NEBNext Ultra II DNA Library Prep Kit and sequenced on an Illumina NovaSeq  (PE 2100 bp) at an average depth of 20 million reads per sample.