GSM9642815: K562 cells, parental-1 IR-4h; Homo sapiens; OTHER
CUT&RUN assay was performed using Assay Kit from Cell Signaling (#86652). The DNA was purified using DNA spin column (Cell Signaling #14209).   End Repair, A-Tailing, and Adapter ligation was performed on 50ng of DNA or 50µl volume equivalents (Roche, KK8501). Adapter ligated DNA was purified using AMPure XP beads (Beckman Coulter, A63881) and PCR amplified (Roche, KK2602) with dual indexing Illumina compatible primers to generate a sequencing library.  Final libraries were again purified and size selected using AMPureXP beads, quantitated by QuBit (Life Technologies, Q33231), size distributions determined by bioanalyzer (Agilent 2100), pooled at equimolar ratios,