GSM8964414: IMR90, RIP-DDX1, OIS, biol rep 1; Homo sapiens; RIP-Seq
For RIP-seq, Magna RIP™ RNA-binding protein immunoprecipitation kit was used. Briefly, cells lasytes were incubated with DDX1 antibody or isotype IgG, the immunoprecipitated RNA was extracted using phenol : chloroform : isoamyl alcohol (125:24:1) . For RIP-seq,  libraries were constructed using Illumina Stranded Total RNA Prep with Ribo-Zero Plus kit (Illumina) following the manufacturer's instruction.