GSM9248803: E. coli infected by P- phage at high MOI, 45min, rep2; Escherichia coli str. K-12 substr. MG1655; RNA-Seq
Cell lysis was performed by resuspending the cell pellets in TE buffer containing 1 mg/mL lysozyme and incubating for 5 minutes at room temperature. Subsequent steps—including removal of cell debris, ethanol precipitation of RNA, DNase I treatment, washing, and RNA elution—were carried out using the Quick-RNA Fungal/Bacterial Microprep Kit (Zymo Research) according to the manufacturer's protocol. The total RNAs were converted into individually barcoded RNA-seq libraries without rRNA depletion with the RNA Library Prep kit from Watchmaker Genomics. Sequencing was performed on a single 10B lane of a NovaSeq X Plus, generating 100-nt single-end reads.