GSM8974190: NPM1 patient 03+05, diagnosis sample,protein; Homo sapiens; OTHER
Cryopreserved bone marrow mononuclear cells were thawed, washed with PBS, filtered through a 40-μm cell strainer, and counted using an automated cell counter. Approximately 140,000 viable cells were loaded onto the Tapestri V3 platform. Single-cell DNA + protein sequencing was conducted using the Tapestri platform (MissionBio) with a customized amplicon panel based on sample-matched WXS data. 45 surface proteins were targeted using the “TotalSeq-D Heme Oncology” panel (Biolegend). Two donor samples with at least 3 “opposite” homozygous SNPs were multiplexed.