GSM9352921: CRS and HTVi of ΔN90-β-Catenin/c-Met/SBT, snRNA-seq; Mus musculus; RNA-Seq
For snRNA-seq, the 10× chromium pipeline was applied. Mice were perfused with 0.9% NaCl solution to remove blood cells. Mouse livers were immediately snap-frozen in liquid nitrogen and stored at -80°C before use. Nuclear isolation was performed on ice using nuclear lysis buffer followed by filtration, centrifugation, and washing. After nuclear isolation and quality control, single-nucleus libraries were prepared according to manufacturer protocol of Chromium Next GEM Automated Chip G Single Cell Kit (10× Genomics).