CUTTAG
library_strategy is Cut&Tag-seq. 50,000150,000 U2OS cells were used for permeabilization and overnight antibody incubation at 4 C, then nuclei were collected and incubated with pA-Tn5 at room temperature for 1 h followed by Mg2+-triggered tagmentation. Fragmented DNA was isolated using phenolchloroformisoamyl alcohol and the final sequencing library was amplified using i5 and i7 indexed primers.