GSM8843397: Achilles tendon_lunar_2; Homo sapiens; RNA-Seq
Total RNA was extracted from the Achilles tendons, quadriceps femoris muscles, and brown adipose tissue. These tissues were homogenized using zirconia beads in TRI Reagent (Cosmo Bio, Tokyo, Japan). Total RNA was then extracted using the Direct-zol RNA Kit (Zymo Research, CA, USA) according to the manufacturer's protocol. Sequence libraries were prepared using the SMART-Seq mRNA (Takara Bio, Japan) and Nextera XT DNA Library Preparation Kit (Illumina, USA) according to the manufacturer's protocol.