GSM9636845: Pt6_Bx_PreIFNg_VDJ; Homo sapiens; OTHER
Cryopreserved PBMCs were thawed and washed in PBS with 0.04% BSA. Tumor biopsies were enzymatically dissociated, washed, and subjected to dead cell removal. Cells were counted and resuspended for single-cell capture. TCR V(D)J libraries were generated using 10x Genomics Chromium Single Cell V(D)J Reagent Kits according to the manufacturer's instructions.