GSM8964424: IMR90, CytoDRIP, Pro-S9.6, biol rep 2; Homo sapiens; OTHER
For cytoDRIP-seq and nuclear DRIP-seq, the cytoplasmic or nucleoplasmic fractions were pretreated with proteinase K and RNase A treatment followed by incubation with S9.6 antibody or isotype IgG, elueted samples were snicated using Covaris ME220 machine. For cytoDRIP and nuclear DRIP seq, libraries were constructed using xGen ssDNA & Low-Input DNA Library Prep Kit (IDT)  following the manufacture's instruction.