GSM9395945: HFD_p53KO_6; Mus musculus; RNA-Seq
Flash frozen liver tissues were used to extract RNA using Quick-RNA Miniprep Plus Kit (Zymo) following manufacturer's protocols. RNAseq polyA RNA isolation and library preparation was performed with the Watchmaker mRNA Library Prep Kit (Watchmaker Genomics: 7BK0001 ) with xGEN Stubby adaptors (IDT, Cat: 336338420) and xGEN 10nt UDIs (IDT, Cat: 336338436). Libraries were sequenced with the Element Biosciences AVITI Sequencing platform using the AVITI 2x75 High Output Cloudbreak Freestyle Kit (Element Biosciences, Cat: 860-00015).