Spatial transcriptomics of adipocyte-specific hM4Di-DREADD expressing mice 24 h after cardiac ischemia.
Spatial transcriptomics of adipocyte-specific hM4Di-DREADD expressing mice 24 h after cardiac ischemia.
After mouse sacrifice hearts were excised and briefly washed in ice-cold PBS. Hearts were place in OCT, snap-frozen in pre-cooled isopentane (-30 to -40°C) and stored at -80°C until further use. 10µm thick tissue sections were cut in a cryostat at -20°C chamber temperature and -10°C object head temperature and placed at the 10X Visium Gene Expression slide. Further processing was performed as described by the manufacturer. Prepared Visium slides were used as input for the Visium Spatial Gene Expression workflow (10X Genomics, Pleasanton, USA) according to the manufacturer's instructions. Tissue permeabilization was carried out for 12 minutes Library construction was performed to manufacturer's instructions, the Visium Spatial Gene Expression workflow (10X Genomics, Pleasanton, USA).