GSM8955392: hk3,4,5,6 callus line 13 treated with vehicle control; Oryza sativa; RNA-Seq
Tissue was flash frozen in liquid nitrogen and then ground with a mortar and pestle. RNA was extracted from the ground tissue with RNAzol (Sigma, R4533). RNA quantity and quality was assessed using the Agilent 4200 Tapestation System (Agilent, G2991BA). RNA-seq library preparation was performed by Innomics (Sunnyvale, CA, USA). Total RNA was enriched for mRNA using oligo(dT) beads, followed by fragmentation and first and second strand cDNA synthesis. Second strand synthesis incorporated dUTP to enable strand specificity. After end-repair and 3′ adenylation, adaptors were ligated, and libraries were PCR-amplified. Libraries were quality checked, circularized, and amplified into DNA nanoballs (DNBs) for sequencing. Sequencing was performed on a DNBSEQ-T7 platform (MGI Technology).