GSM8250342: Female_1_PBS_Non-injected_P_1; Mus musculus; RNA-Seq
Neuronal nuclei were isolated from mouse cortex using flow cytometry and filtering for an anti-NeuN antibody. Libraries were prepared by the Van Andel Genomics Core from 500 ng of total RNA using the KAPA RNA HyperPrep Kit with RiboseErase. RNA was sheared to an average of 300 bp . Individually indexed libraries were pooled, and 75-bp single-end sequencing was performed on an Illumina NextSeq 500 sequencer. Base calling was done by Illumina NextSeq Control Software (NCS; v2.0), and the output of NCS was demultiplexed and converted to FastQ format with Illumina Bcl2fastq (v1.9.0) bulk RNA-seq on neuronal nuclei.