GSM9103769: PBS treated L6-DRG Rep2; Rattus norvegicus; RNA-Seq
RNA was isolated via phenol chloroform separation followed by purification of the aqueous/ethanol fraction using an RNeasy 96 plate (Qiagen) according to standard kit protocol, using a vacuum manifold. Library preparation was performed using the NEBNext® Ultra™ II RNA Library Prep Kit using poly(A) enrichment.  Samples were barcoded and multiplexed, and paired end 150 bp (2 x 150bp) sequencing was executed with an Illumina NovaSeq machine.