GSM8966218: TT-seq in dTAG13-treated (24hr) SEM cells with MYB endogenously tagged with FKBP12(F36V), replicate 2; Homo sapiens; RNA-Seq
5e7 4sU-treated cells were lysed in 10ml Trizol. RNA was extracted using chloroform and precipitated with EtOH. RNA was mildly fragmented by sonication. 4sU RNA was biotinylated with 1mg/ml Biotin-HPDP and nascent RNA was enriched for using streptavidin-coated beads. DNA libraries were prepared using the NEB Next Ultra II Directional RNA library preparation kit for Illumina (E7760).