GSM9372614: young replicate 4; Mus musculus; RNA-Seq
Nuclei were isolated, cleaned and sorted using a SH800 (Sony) flow cytometer by the Center for Epigenomics at UC San Diego. Libraries were generated using the Chromium Single-Cell HT 3′ Library Construction Kit v3.1 (10x Genomics, 1000350, 1000351, 1000352) with the Chromium Single-Cell M Chip Kit (10x Genomics, 1000349) and the Chromium i7 Dual Index Kit TT Set A 96 rxns Kit for sample indexing (10x Genomics, 1000215) according to manufacturer specifications.