RNA-seq of mouse immunity induced by empty plasmids
Total RNA was extracted and subjected to poly-A selection for mRNA enrichment. Strand-specific RNA-seq libraries were prepared using the Illumina TruSeq Stranded mRNA Library Prep Kit. Briefly, mRNA was fragmented, reverse-transcribed into cDNA, ligated with Illumina adapters, and amplified by PCR. Library quality and size distribution were validated using an Agilent Bioanalyzer, and quantification was performed via qPCR.