GSM9696040: VW17c, McA-RH7777, 83-2-27, DMSO, 3 dpi; Rattus norvegicus; RNA-Seq
RNA extraction was done using the NucleoSpin RNA kit (Macherey-Nagel) The RNA sequencing of primary rat hepatocytes was done on the Illumina NovaSeq X Plus platform using the commercial service of Novogene (poly-A enrichment, paired-end 150 base pair reads mode, 20 million reads/sample). For cell line-derived samples, the NEBNext UltraExpress RNA Library Prep Kit (New England Biolabs) and the recommended related reagents were applied for library preparation according to the manufacturer´s instructions. The library was sequenced with P2 reagents (Illumina) on the Illumina NextSeq 1000 sequencing platform.