GSM9247912: hiPSC-derived RPE cells, Treatment, Day D0, Replicate R2; Homo sapiens; RNA-Seq
RNA from cells was isolated using the NucleoSpin RNA kit (Machery-Nagel, Düren, Germany) and final samples were eluted in 40 ul of RNAse free water. Gene expression profiling using RNA-Seq was performed by the University of Iowa Genomics Division using manufacturer recommended protocols. Briefly, 250 ng of DNase I-treated total RNA was used to prepare sequencing libraries using the Illumina stranded mRNA library preparation kit (Illumina, Inc., San Diego, CA, USA). The pooled and barcoded libraries were sequenced on the Element AVITI24 sequencing platform (Element Biosciences, San Diego, CA) using a Cloudbreak FS 150 cycle (2x75 bp) high output sequencing flow cell to generate at least 30M paired-end sequencing reads per sample.