GSM8972649: 78-ON; Homo sapiens; OTHER
Tissue sections (10 μm) were mounted onto Visium Spatial Gene Expression slides (10x Genomics), fand stained with hematoxylin and eosin. Tissue permeabilization was performed according to the manufacturer's recommendations. RNA was captured in situ on the slide and reverse-transcribed to generate spatially barcoded cDNA Libraries were prepared using the 10x Genomics Visium Spatial Gene Expression protocol, following the manufacturer's instructions. Spatially barcoded cDNA was amplified and converted into sequencing-ready libraries. Libraries were sequenced on an Illumina Nova Seq 6000 platform.