Fall army worm and Fusarium graminearum stress response RNA-seq studies across three maize genomes
Fall army worm and Fusarium graminearum stress response RNA-seq studies across three maize genomes
Inner-whorl leaf tissue was harvested which included leaf tissue from 2-3 rolled leaves after two days post-fall army worm and control application. A 4-cm stem section centered on the stack node (includes immature stem, stack node, meristem, and immature leaves) was collected 2 days after inoculation. Temperature controlled greenhouse under normal light conditions. Plants were infested by placing approximately 50 fall armyworm (Spodoptera frugiperda) into the whorl three-week-old plants.Â    Four-week-old plants were inoculated by slitting the stems through the stack nodes and inoculating with 200 uL of Fusarium graminearum spore solution.  RNA was extracted from 100 mg of ground tissue using the Zymo DIRECT-ZOL RNA Kit (Zymo Cat # R2051). rRNA was depleted using NEB depletion kit and libraries were constructed with NEBNext Ultra II directional library kit by the Iowa State University DNA Sequencing Facility.