GSM9699714: SK-Mel-103, H3K27ac, replicate 3 (ChIP); Homo sapiens; ChIP-Seq
Nuclei were sonicated in a Bioruptor to yield chromatin fragments of ~200-500 bp, as confirmed by agarose gel electrophoresis. ChIP-seq libraries were prepared and sequenced using single-end 50 bp sequencing (SE50) on the BGISEQ-500 platform (BGI, Wuhan, China).