GSM9008357: EOM_snRNA_seq; Macaca fascicularis; RNA-Seq
Total RNA was extracted from EOM and QFM tissues of macaca fascicularis, with RNA quality assessed using the RNA Nano 6000 Assay Kit and Agilent 2100 Bioanalyzer. For scRNA-seq, mRNA was isolated via poly-T oligo magnetic beads, fragmented, and first-strand cDNA synthesized. For snRNA-seq, nuclei were isolated, and RNA extraction followed similar procedures. cDNA preparations underwent end repair, adaptor ligation, and size selection (~370–420 bp) using AMPure XP beads, followed by PCR amplification. Libraries were assessed for quality using the Qubit 2.0 Fluorometer and Bioanalyzer 2100. Sequencing was performed on the Illumina NovaSeq 6000 platform with 150 bp paired-end reads. Total RNA was extracted from EOM and QFM tissues of macaca fascicularis, with RNA quality assessed using the RNA Nano 6000 Assay Kit and Agilent 2100 Bioanalyzer. For scRNA-seq, mRNA was isolated via poly-T oligo magnetic beads, fragmented, and first-strand cDNA synthesized. For snRNA-seq, nuclei were isolated, and RNA extraction followed similar procedures. cDNA preparations underwent end repair, adaptor ligation, and size selection (~370–420 bp) using AMPure XP beads, followed by PCR amplification. Libraries were assessed for quality using the Qubit 2.0 Fluorometer and Bioanalyzer 2100. Sequencing was performed on the Illumina NovaSeq 6000 platform with 150 bp paired-end reads.