GSM8967663: Mtb_Δrv0998_pH_5.7_biol_rep_2; Mycobacterium tuberculosis; RNA-Seq
Bacteria were harvested by 10-minute centrifugation at 4,000 x g, 4°C. Total RNA was extracted using a FastRNA Pro Blue kit (MP) according to the manufacturer's instructions. The samples were treated with RNase-free Dnase (Promega) for 1 hour at 37°C to remove contamination of genomic DNA. The samples were purified with RNAeasy columns (Qiagen) according to the manufacturer's instructions. The quantity and quality of RNA were assessed using a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific) and an Agilent 2100 Bioanalyzer with an RNA 6000 Nano LabChip kit (Agilent). RNA sequencing was performed using an Illumina HiSeq4000 sequencer in the Imperial BRC Genomics Facility at Imperial College London.