Metabarcoding of tobacco rhizosphere
Tobacco was sown on vermiculite irrigated with Knop solution. At 11 days, half the plants were inoculated with of AMF inoculum and the other half received the same amount of sterile soil. At 28 days, each seedling was transplanted into soil. AMF inoculation was repeated, whereas the pathogen was inoculated using 1 mL of T. basicola spore suspension (or 1 mL of 0.8% NaCl solution was used). The plants were left for another 21 days in a growth chamber with 16h of light (22C) and 8h of darkness (18C), and soil water content was adjusted to 70% water-retention capacity.