GSM8964437: IMR90, MapR-Proliferating-GST; Homo sapiens; OTHER
For MapR-seq, cells were immobilized and incubated with GST-MNase and GST-RH-Mnase followed by addition of CaCl2 for digestion. After quenching, DNA was purified from released chromatin fragments using phonol/chloroform method, and 7 uL of total 20 uL DNA was used for library construction. For MapR-seq, libraries were constructed using NEBNext Ultra DNA Library Prep Kit (NEB) following manufacturer's instruction.