GSM8965598: WT-02; Mus musculus; RNA-Seq
Spleen were mechanically digested using a scalpel and passed sequentially through 16G × 1" and 19G × 1½" needles. Single-cell suspensions were filtered through a 70 µm cell strainer, followed by red blood cell lysis using BD Pharm Lyse buffer (BD Biosciences, Cat# 555899) for 15 minutes. Cells were then washed with PBS and filtered through a 35 µm cell strainer. Single cells were loaded onto 10X Genomics GEM-X chips targeting 10,000 cells per sample, and barcoded cDNA transcripts were generated using the 10X Genomics Chromium X platform. Sample-labeled cDNA libraries were constructed according to the manufacturer's protocol and sequenced on an Illumina NovaSeq X sequencer