GSM8965648: P55-siNTC; Homo sapiens; RNA-Seq
RNA sequencing was performed on RNA extracted and DNAse-treated samples using the miRNeasy mini kit (Qiagen) obtained from five biological replicates of either quiescent, non-transfected (0.2% NT), mock-transfected, IL-PDGF-treated, siNTC-transfected, IL1-PDGF-treated, or BHF7-transfected, IL1-PDGF-treated vSMCs Ribosomal-depleted unstranded libraries were prepared by GENEWIZ, Inc. (South 79 Plainfield, New Jersey, USA) and were sequenced with Illumina HiSeq (paired-end 2x150bp).