GSM9636825: Pt1_PBMC_I2D003_GEX; Homo sapiens; RNA-Seq
Cryopreserved PBMCs were thawed and washed in PBS with 0.04% BSA. Tumor biopsies were enzymatically dissociated, washed, and subjected to dead cell removal. Cells were counted and resuspended for single-cell capture. Single-cell RNA-seq libraries were prepared using the 10x Genomics Chromium Next GEM Single Cell 5' Reagent Kits v2 (Dual Index) following the manufacturer's instructions.