GSM9590030: A24KD; Homo sapiens; RNA-Seq
RNA was isolated using PureLink™ RNA Mini Kit (ThermoFisher) as per manufacturer's instructions mRNA was enriched from total RNA using poly(A) selection with oligo(dT) magnetic beads. The purified mRNA was fragmented under elevated temperature and converted into first-strand cDNA using random hexamer primers and reverse transcriptase. Second-strand cDNA synthesis was subsequently performed to generate double-stranded cDNA. The cDNA fragments were end-repaired, A-tailed, and ligated with Illumina sequencing adapters. Adapter-ligated fragments were PCR amplified and purified to construct the final sequencing libraries. Library quality and concentration were assessed prior to paired-end sequencing