GSM8967601: Input for H3K27ac ChIP-seq in E14 (TOT2N variant) cells containing stable integration of TetR-MYBTA constuct (control); Mus musculus; ChIP-Seq
Cells were washed with PBS and fixed in either 1% FA for 10 minutes (histones) or 2mM DSG for 30 minutes and 1% FA for 30 minutes (TFs). Cells were sonicated to give fragments of 100-300bp followed by IP. DNA libraries were made using the NEBnext Ultra DNA II library preparation kit for Illumina (Cat no. E7645).