16S rRNA V3-V4 seq of WS-PS-B-002
Bowls were swabbed, swab was stored in PrimeStore media. DNA extraction was performed with RNeasy PowerSoil. 16S metabarcoding, including PCR of the V3-V4 variable regions, library preparation, and DNA sequencing, was carried out at Gnome Qubec. Sequencing was done with 600 cycles on an Illumina NextSeq2000 with a target of 100,000 paired end reads per sample.