GSM9707032: Mel202,BAP1 KD,Replicate 1; Homo sapiens; ChIP-Seq
Chromatin immunoprecipitation (ChIP) followed by next-generation sequencing (ChIP-seq) was performed using 20 million cells per experiment, which were crosslinked for 7 min with 1% formaldehyde. Chromatin was sonicated to an average fragment size of 200–500 base pairs with a Covaris M220 sonicator. A measure of 10 μg of antibody was used for each ChIP experiment. Libraries were prepared using the NEBNext Ultra 2 kit and sequenced by the University of Miami Oncogenomics Shared Resource with >20 million reads per sample.