GSM8631057: A549 cells, control FLuc siRNA, IFN, 12h, rep1; Homo sapiens; RNA-Seq
RNA samples were harvested with 500 μL TRI-zol (Invitrogen) and total cellular RNA was extracted according to the Direct-zol RNA Miniprep Kit, including the optional DNase I treatment (Zymo Research, R2052). Samples were prepared following the standard protocol for the Illumina Stranded mRNA prep (Illumina).