GSM9355322: LEM; Mus musculus; RNA-Seq
Following Fc block with anti-CD16/32, cells were stained with CD45-FITC and CD11b-PE for 20 minutes on ice. After washing, DAPI was added to discriminate viable cells, and live microglia (CD45loCD11b⁺DAPI⁻) were sorted using a FACSAria II (BD Bioscience) directly into 1% BSA in PBS. Sequencing libraries were prepared using the 10X Genomics Chromium Single Cell 5' PE Library & Gel Bead Kit V2