RNA-Seq of acute myeloid leukemia cell line: THP-1
Total RNA of THP-1 cells was extracted using Trizol in both the co-culture and monoculture groups. The purity and concentration of the RNA were measured using a Nanodrop spectrophotometer (NanoDrop One, Thermo Fisher, USA). Qualified RNA samples were reverse-transcribed to construct the complementary DNA (cDNA) library. The quality of the constructed libraries was assessed to ensure they met the required standards. Next-generation sequencing (NGS) was performed using paired-end (PE) sequencing. After sequencing, raw data were filtered to remove low-quality reads.