HCMV-12hpi HELF cells CAGE-seq
Total RNA was treated with RQ1 DNase (Promega) to remove DNA. The quality and quantity of the purified RNA were determined by measuring the absorbance at 260 nm/280 nm (A260/A280) using Nanodrop (K5500). RNA integrity was further verified by 1.5% agarose gel electrophoresis.