GSM9653331: hiPSC-derived neuron pool (RH and BFP treated, patients 553 and 2607), HTO; Homo sapiens; OTHER
iPSC-derived neurons were dissociated into single-cell suspensions prior to loading onto the 10x Genomics platform. RNA capture and reverse transcription were performed according to the manufacturer's protocol. Single-cell RNA-seq libraries were generated using the 10x Genomics Chromium platform according to the manufacturer's standard protocol. Briefly, single-cell suspensions were loaded for microfluidic partitioning, barcoding, and reverse transcription, followed by cDNA amplification and library construction for sequencing. Hashtag oligonucleotide (HTO) feature barcode libraries were prepared in parallel using standard 10x Genomics-compatible feature barcoding procedures.