Ser5Pol2_CR_R21_Dox2h-Neg6h_Rep1
CUT&RUN was performed using the CUTANA ChIC/CUT&RUN Kit (Epicypher) on RAD21-degron treated with 200 ng/ml dox for 2 hours followed by 0.5 M NEG or dTAGV-1 for 6 hours. Nuclei were extracted, bound to concanavalin A-coated beads, and incubated with specific antibodies overnight. MNase digestion was performed, and DNA was purified using SPRIselect beads. Libraries were prepared with the NEBNext Ultra II DNA Library Prep Kit and sequenced on an Illumina NovaSeq X (PE 275 bp) at an average depth of 20 million reads per sample.