RNA-Seq of Chaptalia nutans: leaves
Leaves preserved in RNAlater Stabilization Solution went through a PBS wash (50 mM potassium phosphate; 150 mM NaCl; pH 7.2) prior to extraction with E.Z.N.A. Plant RNA Kit (Omega Bio-Tek). RNA libraries were prepared from enriched mRNA using oligo(dT) beads with a250~300 bp insert cDNA library using the Illumina TruSeq library preparation kit.