GSM9461891: Ad5-HA-VLP_1_D7; Mus musculus; RNA-Seq
Single cells were suspended in calcium- and magnesium-free PBS containing 0.04% weight/volume BSA. Then Cells were added to each channel. After generation of GEMs, reverse transcription reactions were engaged barcoded full-length cDNA followed by the disruption of emulsions using the recovery agent and cDNA clean up with DynaBeads Myone Silane Beads (Thermo Fisher Scientific). Subsequently, the amplified cDNA was fragmented, end-repaired, A-tailed,  and index adaptor ligated and library amplification. RNA libraries  were prepared with Chromium Single cell 3' Reagent v3 Kits according to the manufacturer's protocol.