GSM8966226: PolyA minus RNA-seq in wild type E14 (TOT2N variant) cells, replicate 1; Mus musculus; RNA-Seq
RNA was extracted from cells using the RNeasy Mini Kit (Qiagen). PolyA-minus RNA was isolated using the NEBNext Poly(A) mRNA magnetic isolation module (retaining the unbound fraction). DNA libraries were prepared using the NEB Next Ultra II Directional RNA library preparation kit for Illumina (E7760).