GSM9347021: A_2; Rattus norvegicus; RNA-Seq
Upon completion of the in vivo experiments, intervertebral disc tissues were harvested from both the degenerative group and the B2M-ApoEV/DFS@HMs-treated group, followed by meticulous isolation of NP tissues. The NP specimens were uniformly minced into approximately 0.5 mm³ fragments and subsequently subjected to enzymatic digestion to obtain single-cell suspensions. Cell concentration and viability were quantitatively assessed using a Luna automated cell counter. Single cell RNAsequencing was conducted by Shanghai OE Biotech Co., Ltd. utilizing the BD Rhapsody™ platform. Following the manufacturer's recommendations, the single cell suspension was adjusted to the appropriate volume for loading and capture using the BD Rhapsody™ Enhanced Cartridge Reagent Kit and BD Rhapsody™ Cartridge Kit. Reverse transcription was performed using the BD Rhapsody™ cDNA kit. BD Rhapsody™ WTA Amplification Kit was used for DNA library construction. High-throughput sequencing was performed with the PE-150 mode.