GSM9703187: HUVEC, CD109 siRNA transfection, replicate 1; Homo sapiens; RNA-Seq
Total RNA was isolated from n=3 scramble control and CD109 siRNA treated HUVEC cultures using the RNeasy Micro Kit (QIAGEN) with RNase free DNAse digestion (QIAGEN) to prevent genomic DNA contamination according to manufacturer's protocol. Total RNA samples were submitted to a commercial provider (Genewiz-Azenta) for paired-end sequencing at 150bp read length and 20-30 M reads per sample.