Single-cell RNAseq of PBMC from mice infected with Dengue virus
Single-cell RNAseq of PBMC from mice infected with Dengue virus
Blood was centrifuged at 6,000 g for 10 minutes. Red blood cells were subjected to lysis using BD Pharm Lyse™ Lysing Buffer (BD). Dead cells were removed with dead Cell Removal kit (Miltenyi Biotec).  Samples were fixed overnight at 4°C and stored at -80°C, according to the 10x Genomics Demonstrated Protocol CG000478. Fixed cells were hybridised overnight with both mouse whole transcriptome amplification (WTA) probes and spike-in custom probe pool using Chromium Fixed RNA Profiling Kit (10x Genomics). Single cell RNA sequencing libraries were then generated using Chromium Fixed RNA Profiling Kit (10x Genomics).