RNA-seq of mouse neurons in mono-culture and in co-culture with neurons, with application of Nrf2 activator tBHQ.
RNA-seq of mouse neurons in mono-culture and in co-culture with neurons, with application of Nrf2 activator tBHQ.
"Neurons and astrocytes were cultured as described in Bell et al., \""Neuronal development is promoted by weakened intrinsic antioxidant defences due to epigenetic repression of Nrf2\"", Nature Communications 6: 7066 (2015)." Pure neuronal cultures or mixed astrocyte/neuronal cultures were treated ± tBHQ (10 µM) for 8h. At this point RNA was extracted and subject to RNA-seq analysis. RNA-seq libraries were prepared by Edinburgh Genomics using the Illumina TruSeq stranded mRNA-seq kit, according to the manufacturer's protocol (Illumina).