16S rRNA gene V3-V4 amplicon sequencing of insect gut microbiome: gut bacterial communities of two earwig species from agricultural habitats in Guizhou, China
Gut bacterial communities of two earwig species (Eudohrnia metallica and Timomenus komarovi) were collected from mulberry and maize fields in Guizhou Province, China. Genomic DNA was extracted from insect gut contents, and the V3-V4 hypervariable region of the 16S rRNA gene was amplified by PCR. Sequencing libraries were constructed and sequenced on the PacBio Sequel IIe platform using single-molecule real-time (SMRT) sequencing.