GSM9516532: P0_day3, rep3; Mus musculus; RNA-Seq
Total RNA (15 ng) was extracted from Sa-SVF cells using an ISOSPIN Cell & Tissue RNA Kit (Nippon Gene, Tokyo, Japan) or NucleoSpin RNA XS (Macherey-Nagel, Düren, Germany) for small sample volumes. The quality of the RNA was verified (RNA integrity number > 8) using a Bioanalyzer (Agilent 6000 Nano Assay, Agilent Technologies, Santa Clara, CA, USA). Total RNA was amplified to produce cDNA, and cDNA libraries for RNA-Seq were constructed using a SMART-Seq HT PLUS Kit (R400749; Takara Bio USA, CA, USA).