Amplicon sequencing of soil metagenome: Contaminated Site3 replicate 2
Soil samples were collected from pesticide exposed and non contaminated agricultural sites in Dehradun India. Total genomic DNA was extracted using the Qiagen DNeasy PowerSoil Pro Kit. DNA quality and concentration were assessed using agarose gel electrophoresis Nanodrop and Qubit fluorometer. The V3 V4 region of the 16S rRNA gene was amplified using Illumina adapter linked universal primers. PCR products were purified and indexed libraries were prepared using a second round of amplification. Libraries were quantified pooled and sequenced on the Illumina MiSeq platform using paired end sequencing. Raw sequence data were generated in FASTQ format for downstream microbial community analysis.