GSM9703610: Eco RNAP on Cdf Genome R1; Clostridioides difficile; RNA-Seq
RNA transcripts generated from in vitro transcription reactions were purified using standard phenol-chloroform extraction followed by ethanol precipitation. RNA libraries were prepared using a 3′ adapter ligation-based protocol. Briefly, RNA fragments were ligated to a pre-adenylated DNA adapter using T4 RNA ligase 2 (truncated), followed by reverse transcription, circularization, and PCR amplification. Libraries were sequenced on an Illumina platform.