GSM8026448: mESC_d4_IP_CTCF_rep3; Mus musculus; ChIP-Seq
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody (CTCF (abcam, Cat. #ab128873), MBD3 (abcam, Cat. #ab157464),TET1 (GeneTex, Cat No. GTX125888),TET2 (GeneTex, Cat No. GTX124205)). Sequencing libraries were made following the protocol of NEB Next ChIP-Seq DNA Sample Prep Kit with minor modifications. To perform end repair of DNA fragments, 12 μL mixture (5µL NEB T4 DNA ligase buffer with 10mM ATP, 2µL dNTPs mix 10mM each, 2µL T4 DNA polymerase 3u/µL, 1µ