RNA-seq profiling of Listeria monocytogenes infected macrophages lacking Irf2, Irf9, or Irf2 and Irf9, as well as scramble controls
RNA-seq profiling of Listeria monocytogenes infected macrophages lacking Irf2, Irf9, or Irf2 and Irf9, as well as scramble controls
Macrophages lacking Irf2, Irf9, or Irf2 and Irf9, as well as scramble controls were infected with Listeria monocytogenes or uninfected under mock conditions and total RNA was harvested 2 hours post infection. Three independent experiments were performed. Gene knockouts were generated via cas9 guide transduction into cas9-expressing cells.  Total RNA was isolated using TRIzol (Fisher) and Chloroform, and the PureLink RNA Mini Kit (12183018A, Ambion). RNA was then DNase I treated (NEB), and purified using RNA clean and concentrator columns (Zymo). PolyA mRNA enrichment and library preparation was performed by Novogene Corporation Inc.