GSM9706492: Curcumin Treated Visceral Adipose Tissue 2; Mus musculus; RNA-Seq
Total RNA was extracted using RNeasy Lipid Tissue Mini Kit (74804, Quiagen) protocol. RNA concentration and purity were determined using NanoDrop, and integrity was confirmed using an Agilent 2100 Bioanalyzer (RIN values > 7.0 for all samples). RNA-seq libraries were prepared using the Illumina TruSeq Stranded mRNA Library Prep Kit (Poly-A enrichment). High-throughput sequencing was performed on an Illumina NovaSeq 6000 platform (Novogene Co., Ltd.) generating paired-end 150 bp (PE150) reads with an average depth of 20-30 million reads per sample.