Nanopore amplicon sequencing of nalidixic acid enrichment cycle 1 spacer library
The colonies from the first enrichment cycle on the MIC were grown overnight on LB at 37 C. Plasmids were then isolated from the culture, and the enriched spacers were PCR amplified using primers MG7R and TM137F. 50 L of the amplified product was run on 2% (w/v) agarose gel. The single amplified band of 171-173 bps of new spacers was cut from the gel and deep sequenced.