GSM6632727: hESC_H9_d9_WT_RNA_Seq_rep1; Homo sapiens; RNA-Seq
Total RNA was isolated using the MiniBEST Universal RNA Extraction Kit (TaKaRa, Japan). RNA concentration was measured by a Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific).1.3 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using LM-Seq Library Prep Kit following manufacturer's protocols.