RNA-seq of SETD5 W834C heterozygous mouse brains with matched controls
RNA-seq of SETD5 W834C heterozygous mouse brains with matched controls
The frontal cortex was sectioned from the mice brain. Total RNA was isolated from mouse frontal cortex using Qiazol lysis reagent followed by purification with the RNeasy Mini kit (Qiagen) according to manufacturer protocol. DNA was removed using the TURBO DNAfree kit (Ambion) and RNA integrity was assessed on a bioanalyzer. 4Âµg RNA were used and ERCC Spike-ins were added to prepare sequencing libraries using the ScriptSeq Complete Gold Kit (Illumina). ScriptSeq Index Primers (Illumina) were used to multiplex libraries.