GSM9004970: IFN8-LineD-Dif2-Sample1; Homo sapiens; RNA-Seq
RNA was extracted with TRIzol per manufacturers instructions and further purified by Qiagen Rneasy Plus system.  3 spheres were pooled and harvested together to form a single biological replicate which are reported here as a single sample.  5 ng of total RNA was used as input for library preparation. Libraries were prepared for sequencing from total RNA using the SMART-Seq v4 Ultra Low Input RNA Kit (Takara) and amplified 16 cycles by PCR.  A Nextera XT DNA Library Preparation Kit (Illumina) with size selection by AMPure XP beads was used to obtain libraries with a mean size of approximately 500 bp.  Libraries were evaluated for quality on an Aligent 2100 Bioanalyzer and were pooled for sequencing.  Samples were sequenced on an Illumina NovaSeq 6000 via a single lane SP100 Cycle.