GSM8981963: Nasal-TT-F1; Homo sapiens; RNA-Seq
The fully differentiated cells on the inserts were washed three times with PBS and then lysed in TRIzol (Invitrogen) for RNA extraction. The isolated PBMCs were frozen in a BamBanker cell freezing medium (Bulldog Bio). The stored PBMCs were thawed in RPMI medium and washed with PBS and then lysed in TRIzol for RNA extraction. Total RNA was extracted using chloroform phase separation followed by purification using Direct-zol RNA Microprep (Zymo Research) according to the manufacturer's instructions. The samples were all DNaseI treated. PBMC RNAseq libraries of polyadenylated RNA were prepared using the NEB Ultra II Directional RNA Library Prep following manufacturer's ptotocols. Nasal cell RNAseq libraries of polyadenylated RNA were prepared using the Illumina Stranded mRNA Prep following manufacturer's ptotocols.