GSM9706362: Diabetic Hypothalamus 1; Mus musculus; RNA-Seq
Total RNA was extracted using the Trizol reagent (Invitrogen) protocol. RNA concentration and purity were determined using NanoDrop, and integrity was confirmed using an Agilent 2100 Bioanalyzer (RIN values > 7.0 for all samples). RNA-seq libraries were prepared using the Illumina TruSeq Stranded mRNA Library Prep Kit (Poly-A enrichment). High-throughput sequencing was performed on an Illumina NovaSeq 6000 platform (Novogene Co., Ltd.) generating paired-end 150 bp (PE150) reads with an average depth of 20-30 million reads per sample.