WGS of C. auris B11221 after propiconazole exposure
Genomic DNA (gDNA) was extracted using the QIAamp PowerFecal Pro DNA kit . The total gDNA extracted was quantified using a Nanodrop One . The library was prepared using the NEBNext Companion Module for Oxford Nanopore Technologies (New England Biolabs). The adapters were added using the SQK-LSK109 sequencing kit (Oxford Nanopore Technologies ). Purification was performed at each step using NucleoMag NGS Clean-up and Size Select magnetic beads . The prepared library was loaded onto a Flow Cell FLO-MIN114 R10 (ONT).