WGS of Escherichia coli: Duck Meat
Genomic DNA was extracted from overnight cultures of ESBL-producing Escherichia coli isolates using the Maxwell culture DNA extraction kit and Maxwell automated nucleic acid extraction system (Promega), following the manufacturers instructions. DNA concentration and purity were assessed using a Qubit 2.0 fluorometer and NanoDrop spectrophotometer. Tagmentation-based library preparation using Illumina Nextera XT kit and sequenced on the Illumina NextSeq500 platform with 150 bp paired-end reads.