targeted rnaseq of fetal rat heart
Purified RNA underwent rat, whole transcriptome targeted RNA-Sequencing (Templated Oligo assay with Sequencing readout: TempO-Seq). TempO-Seq relies on the hybridization of two, 25 base pair detector oligos (DOs) to adjacent target sequences such that when properly hybridized they can be ligated. The DOs have universal primer sites. There are 22253 DOs targeting 20922 genes. Excess unhybridized DOs are removed enzymatically and then the ligated DO pairs are amplified with the universal PCR primers that contain a sample index sequence and the adapter for Illumina instruments.