Mutation profile of OsWSL5 with CBE_CGRS55  R 03
PCR products subjected to deep amplicon sequencing. After illumina adapter ligation and library preparation, the samples were sequenced using a 2  250 paired-end configurations. Image analysis and base calling were conducted. Raw sequence data were demultiplexed using bcl2fastq version 2.17.1.14. Read pairs were trimmed for adapter sequences and low-quality basecalls using Trimmomatic version 0.36. Each read pair was then merged using the bbmerge tool from the BBtools software toolkit.