metatranscriptomic profile of plankton collected during and Ocean Alkalinity Enhancement Field trial
The Ocean Alkalinity Enhancement trial is described in Wynn-Edwards et al., 2025.  During the Ocean Alkalinity Enhancement field trial, water was continuously pumped into a containerised laboratory, and at discrete time points, aliquots were collected for molecular characterisation.  Plankton were collected on a 1 RNA for RNASeq were extracted from the same samples using the TRIzol extraction protocol (Invitrogen) followed by a column clean up using Qiagen RNeasy kits (cat. no. 74104) as directed by the manufacturers protocol.  Briefly, frozen filters were added to MP Biomedicals Lysing Matrix C bead tubes and resuspended in 500 l custom lysis buffer (described in (Osborn and Hook, 2013).  RNA quality was determined using a Agilent bioanalyser (Agilent RNA 6000 Nano kit (part no. 5067-1511)).