Nanopore sequencing of the mtSSUrRNA gene of Pneumocystis sp. in a foal
The gene encoding the small subunit of the mitochondrial rRNA (mtSSUrRNA) of Pneumocystis sp. was amplified from lung tissue samples, using an end-point PCR assay. The PCR products were purified and sequenced using the ligation sequencing amplicon protocol from Oxford Nanopore Technologies (SQK-NBD114.96), on a MinION Mk1C device.