NGS of TtAgo cleavage products under gDNA-S5 without dCTP/TthSSB
The procedure consisted of three main steps: (i) multi-cycle linear amplification using a single-primer panel; (ii) ligation of the amplified single-stranded DNA product with a single-stranded adapter containing an index sequence; and (iii) indexing PCR to amplify the ligated product using index primers. The resulting target-enriched libraries were further amplified with a universal P7 primer (with index) and a P5 primer (with index), and then purified before sequencing on the NovaSeq 6000 System (Illumina, San Diego, CA, USA).