RNA-Seq of LTH leaves
To explore the resistance mechanism in IRBLta2-Re, this study compared LTH with its resistance monogenic line (IRBLta2-Re) in response to rice blast infection. Seedlings of susceptible and resistant rice varieties were grown under identical greenhouse conditions with three biological replicates. A Magnaporthe oryzae strain H7 was isolated from infected leaves of rice and purified on prune agar media. A three-week-old plants of both varieties were inoculated by spraying method with 1x105 conidial suspension of M. oryzae strain, and distilled sterilized water (ddH2O) were spray as the mock (control) plants of LTH and IRBLta2-Re. Afterward, the inoculated and un-inoculated plants were placed in the black (dark) chamber with 100% humidity at 250C for 24 hours, and then were transferred to greenhouse. The inoculated and mock samples were collected on the basis of two times interval such as; 48-hour post inoculation (hpi) and 96 hpi