mRNA sequence of beta vulgaris: Leaf
Ribosomal RNA depletion was performed for mRNAseq libraries using the Ribo-Zero rRNA Removal Kit (Illumina, San Diego, CA, USA). Poly(A) mRNA was purified using oligo-(dT) magnetic beads. Poly(A) RNA was fragmented using a divalent cation buffer at elevated temperature. This was followed by reverse-transcription to produce cDNA that was used to produce U-labeled second strand DNA. Final libraries were made following end repair, 3 adenylation, adapter ligation, and PCR. For quantification and quality control of mRNAseq libraries, Bioanalyzer 2100 was used. The libraries were sequenced on Illuminas NovaSeq 6000 sequencing platform using paired-end (150 bp) sequencing approach.