Homo sapiens (JCVblood-2023-10-26) ONT V14 HAC WGS: male blood
Genomic DNA was quantified by Qubit DNA Assay (ThermoFisher, Waltham, MA).  ONT library construction was performed in accordance with protocol SQK-LSK114, incorporating a light DNA shearing step with g-tubes (Covaris, Woburn, MA).  Sequencing was carried out using the R10.4.1 flowcell on the PromethION24 sequencer (96 hr run). High accuracy basecalling (HAC) was performed on the PromethION machine with Dorado basecall server v7.4.14 using model dna_r10.4.1_e8.2_400bps_hac@v4.3.0.