RNA-Seq of mus musculus: the contents of the mouse colon
Total genomic DNA was extracted from 30 fecal samples (n = 6 per group) using the E.Z.N.A. soil DNA Kit (Omega Bio-Tek). PCR amplification was performed in triplicate using Fast Pfu polymerase, and the purified amplicons were pooled and paired-end sequenced on an Illumina Nextseq2000 platform