RNA-Seq of Mus musculus: tumor
This study examines the transcriptional impact of sphingosine kinase 2 (SphK2) deficiency in myeloid-derived suppressor cells (MDSCs) generated in vivo during tumor progression. MDSCs were isolated directly from tumor-bearing Sphk2 wild-type (WT) and Sphk2 knockout (KO) mice to capture genotype-dependent differences under physiologic tumor-driven immunosuppressive conditions. Total RNA was extracted from freshly sorted tumor-derived MDSCs and subjected to RNA sequencing to define global gene expression programs regulated by SphK2. Biological replicates were included for each genotype to ensure robustness and reproducibility of downstream differential expression analyses.