Bovine vaginal metagenome
DNA was randomly fragmented and processed through end-repair, A-tailing, and ligation of Illumina sequencing adapters. Adapter-ligated fragments were size-selected and PCR-amplified to generate sequencing libraries. Library concentration was quantified using a Qubit fluorometer and qPCR, and fragment size distribution was assessed using a fragment analyzer. Libraries were pooled and sequenced on an Illumina NovaSeq X Plus platform (Illumina Inc., San Diego, CA, USA) using paired-end 150 bp reads (PE150).