WGS of experimental evolution mutator lines
For each population size class and mutator background, we selected five endpoint isolates, each originating from a different, randomly chosen population. Single colonies were grown overnight in 5 mL LB broth, and genomic DNA was extracted with the DNeasy UltraClean Microbial Kit (Qiagen, Germany). DNA from the five isolates in each treatment was combined into a single, equally weighted pool for deep sequencing.