WES of mus musculus: male pancreas
DNA was isolated from cells or snap frozen pancreas/tumor tissue using the DNeasyBlood and Tissue Kit (Qiagen) and quantified with the Qubit dsDNA HS Assay Kit(Invitrogen) according to the manufacturers instructions. 10 ng of genomic DNA (gDNA)was mixed with 1x QuantStudio 3D Digital PCR Master Mix v2 and 1x KRASG12D TaqMandPCR Liquid Biopsy Assay (Applied Biosystems A44177, Hs000000051), loaded ontoQuantStudio 3D Digital PCR 20K chips (Applied Biosystems), and analyzed with theQuantStudio 3D Digital Real-Time PCR System (Applied Biosystems) and QuantStudio3D Analysis Suite software (Applied Biosystems). A no template control was used toestablish thresholds for the assay.Mouse Exome panel (Twist Biosciences) was used for whole-exome sequencingaccording to manufacturers instructions. Samples were sequenced with theNovaSeq6000.