Spatial Transcriptomics of MASLD Mouse Liver
Spatial transcriptomics Gene Expression Kit (BMKMANU, ST03002) and Tissue Optimization Kit (BMKMANU, ST03003) were used according to manufacturer instructions.Sequence libraries were then processed according to manufacturers instructions (BMKMANU, Library Construction Kit, ST03002-34). Libraries were prepared with Truseq Illumina libraries and sequenced on a NovaSeq 6000(Illumina).Sequencing was performed with the recommended protocol , yielding between 300 million and 400 million sequenced reads.