Raw reads: 58-CCMP2758-20C-t2-R3
Experimental design description: Differential gene expression analysis of cultures grown at different temperatures (20C and 7C) and harvested at different growth stages. Library_layout: PAIRED. Library design: stranded polyA RNA-seq. Library construction protocol: TruSeq Stranded mRNA polyA library preparations were carried out. Fragments were barcoded using Unique Molecular Identifiers (UMIs) (9 bp length) during library preparation. Identifiers were originally in a separate FastQ file. Then the raw FASTQ file was modified to add the UMI identifiers to the FastQ header.