RNA-seq cucurbita pepo
RNA-seq libraries were prepared starting from 2.5 ?g of total RNA using the TruSeq RNA Sample Prep Kit v2 (Illumina Inc., San Diego, CA, USA). The libraries were then size-selected with a Pippin Prep (Sage Science Inc., Beverly, MA, USA) resulting in a selected range of about 250?350 bp. The cDNA libraries were sequenced with TruSeq Sequencing by means of a Synthesis Kit v3-HS and TruSeq Paired End Cluster Kit v3-cBot-HS (Illumina Inc., San Diego, CA, USA) using an HiSeq 1000 (Illumina Inc., San Diego, CA, USA) sequencer according to the manufacturer?s instructions to generate 100-bp paired-end reads.