meCLIP of EIF4A3: AffinityScript (readthrough)
UV-crosslinking and immunoprecipitation using anti-HA antibody on CRISPR-tagged HeLa cells. Ligation of 5'-biotinylated linker. RT performed using TGIRT III followed by NextSeq Illumina sequencing. Reads with the 5'-linker were obtained and named "readthrough" reads.