Duplex Sequencing of H. sapiens
Genomic DNA from purified CD19+ B-cell was fragmented by sonication, end-repaired and A-tailed.  Purified DNA fragments (~250 bp) were ligated with DS adaptors containing unique molecular barcodes comprising 12 random nucleotides.  The ligated DNA was PCR amplified and purified amplicons were hybridized to 5-biotinylated capture probes.  A process of double capture was performed to improve on-target capture efficiency.  After purification by binding to streptavidin beads, the final PCR amplified the captured DNA library using distinct index primers for each sample to enable multiplex sequencing.  The prepared libraries were mixed and sequenced by Illumina HiSeq2500.