RNA-Seq of Avocado
Poly(A)-RNA was isolated using oligo(dT)  magnetic beads (Invitrogen Dynabeads). 0.5-1 g of poly(A)RNA were then  converted into full-length-enriched double stranded cDNA using the Mint-2  cDNA synthesis kit and following the manufacturers instructions (Evrogen).  The double stranded cDNA was then normalized using the DSN-based Trimmer-2  cDNA normalization kit and following the manufacturers instructions  (Evrogen). The normalized cDNA libraries were then sheared into ~300 bp  fragments with a sonicator (Bioruptor, Diagenode) and indexed with adaptors  using technology NEBNext DNA Library Prep Master Mix Set for Illumina