Genomic DNA from Cystoisospora suis oocysts
Cystoisospora suis oocysts (strain Wien I) were isolated from experimentally infected piglets, left to sporulate in potassium bichromate and purified using a caesium chloride gradient (Worliczek et al., 2010b). DNA was extracted from 2.5×106 washed and pelleted sporulated oocysts using a Peqlab Microspin tissue DNA kit (Peqlab, Erlangen, Germany) following the manufacturer’s instructions. RNAse A digestion was performed on the DNA before final purification.