ChIP-seq of cardiac and embryonic stem cells
ChIP-seq was performed from FACS-sorted cardiac myocyte nuclei (Gilsbach et al., Nat. Commun. 2014). For immunoprecipitation (used antibody: anti-SMC1; biomol, A300-055A), the iDeal ChIP-seq kit for Transcription Factors (Diagenode) was used with 1µg of chromatin according to manufacturer's instructions. Sequencing libraries were prepared from the resulting DNA with the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB). To avoid over-amplification of the sequencing library, test qPCR-amplification was carried out to determine minimum cycles for final PCR.