GSM4654894: SST-TdT-X94_P45_tdt2; Mus musculus; RNA-Seq
cDNA libraries were prepared from the isolated cells using the Chromium Single Cell 3' System according to manufacturer's instructions, with the sole following modification: The quantified cell suspension was directly added to the reverse transcription master mix, along with the appropriate volume of water to achieve the approximate cell capture target. We omitted the 0.04% weight/volume BSA (400 µg/ml) washing step to avoid inevitable cell loss. 2470 tdT +and 1100 GFP +cells were applied to individual channels of the Single Cell 3' Chip. The completed libraries were sequenced an Illumina HiSeq4000 to produce paired-end 100nt reads.