GSM4155920: MmDG-Sox11-4; Mus musculus; RNA-Seq
The dentate gyrus (DG) was microdissected from unfixed brain tissue. Mice were killed by cervical dislocation and the DG was dissected as described (Hagihara, Toyama et al. 2009). The prepared tissue was stored in stabilizing RNAlater buffer (Qiagen, Cat No./ID: 76154) until RNA was isolated using the RNeasy Kit (Qiagen, Cat No./ID: 74106) according to manufacturer's instructions. Barcoded RNA sequencing libraries were prepared from 100 ng total RNA using TruSeq Stranded mRNA Sample Preparation Kit, according to the manufacturer's instructions (Illlumina).