GSM2719117: CRABP2-2; Homo sapiens; RNA-Seq
Total RNA was extracted with Quick-RNA mini kit (Zymo Research) PolyA mRNA libraries were prepared from total RNA using the KAPA mRNA HyperPrep Kit (Kapa Biosystems). Final libraries were quantified using the Qubit 2.0 Fluorometer (Thermo Fisher Scientific Inc.), and the fragment size distribution was determined with the Bioanalyzer. The libraries were then pooled equimolarly, and the final pool was quantified via qPCR using the Kapa Biosystems Library Quantification Kit, according to manufacturer’s instructions.