RNA-seq of Tracheliastes polycolpus: adult female trunk
Total RNA was extracted using the RNeasy Plus Mini Kit following the manufacturers instructions with a final elution volume of 40 L RNAse-free water. Nanodrop ND-8000 and a BioAnalyser were used to check RNA extraction quality and quantity. A Tecan EVO2000 using the Illumina TruSeq Stranded mRNA protocol was used to prepare the individual RNA-seq libraries. Libraries were then pooled and sequenced on two lanes of an Illumina HiSeq 2000 using a paired-end read length of 2x100 bp. The reads were demultiplexed, the adapters were trimmed and a chastity filter was applied.