GSM4476726: Hela_Penet_4hrs_02; Homo sapiens; RNA-Seq
RNAzol was used to isolate total RNA The RNA seq libraries were prepared with TruSeq RNA Library Prep kit v2 as per protocol instructions. Resulting libraries concentration, size distribution and quality were assessed on a Qubit fluorometer with a dsDNA high sensitivity kit and on an Agilent 2100 bioanalyzer using a DNA 7500 kit. Then libraries were normalized, pooled and quantified with a KAPA Library quantification kit for Illumina platforms on a ABI StepOnePlus qPCR machine, then loaded on a high output flow cell and single-end sequenced (100 bp) on an Illumina HiSeq 2500 next generation sequencer.