GSM2546836: +vitC allo-iTregs 1; Mus musculus; RNA-Seq
Total RNA was isolated from purified APCs with RNAeasy Plus mini kit (Qiagen). Purification of poly‑A containing mRNA was done using poly‑T oligo attached magnetic beads (Illumina). Following the purification, the mRNA was used for library preparation using Script Seq v2 Library preparation kit (Illumina).