Drosophila mauritiana Pool-Seq of 107 isofemale lines
Genomic DNA was isolated using the Qiagen DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany) according to the manufacturer*s instructions, and 5 ug were used for construction of paired-end libraries. After shearing the DNA using the Covaris S2 system (Covaris, Inc. Woburn, MA) Illumina paired-end libraries of approximately 280 bp fragment size were generated following the instructions of the Illumina Paired-End Sample Preparation Kit.