Pharate larvae non-diapause replicate 3
Pharate larvae (embryonated eggs) collected from a single biological replicate of Aedes albopoctus females reared under diapause-averting conditions. RNA was extracted by homogenizing tissue in TRI Reagent (Sigma-Aldrich, St. Louis, Missouri, USA) and performing a phenol-chloroform extraction followed by an isopropanol precipitation according to the manufacturerŐs directions. Residual DNA was removed from the sample using TurboDNA Free (Ambion, Austin, Texas). The library was prepared and barcoded using the TruSeq RNA sample preparation kit (Illumina, San Diego, California, USA) at the University of Maryland Institute for Genome Science. The library was run using one-eighth of each of two Illumina MiSeq lanes.