GSM4156666: mesc_hmc_500_K2; Mus musculus; OTHER
Standard DNA phenol-chloroform extraction 5hmC in sonicated  genomic DNA was glycosylated with 6-azide-glucose. After ligation of adapters as described previously (Stasevskij et al., 2017),  the alkyne-bearing oligodeoxyribonucleotide with biotin was attached to the azide moiety of the modified DNA. Biotinylated DNA was enriched with streptavidin-coupled magnetic beads and subsequently used in a DNA priming reaction from the tethed oligonucleotide. Primed DNA library was amplified, size-selected and subjected to Ion Proton sequencing. uTOP-seq libraries were prepared as described in Stasevskij et al, 2017.