GSM4475925: Hippocampus, Kif5C IP_2; Mus musculus; RNA-Seq
Birefly lysates were prepared using a lysis buffer (0.50 % Nonidet P-40, 50 mM Tris-HCl pH 7.5, 125 mM NaCl, 1 mM EDTA pH 8.0, 1 mM DTT, 1 protease inhibitor cocktail tablet (Roche), 100 μl of phosphatase inhibitor cocktail 1 and 2 each (Sigma), 100 μl BSA). Following lysis for 30 minutes at 4 degreeC, lyzates were centrifuged to remove any debris and proceeded to IP. RNA libraries were prepared for sequencing using standard Illumina protocols