GSM3728326: CHD34 [2017_007_015]; Homo sapiens; RNA-Seq
Total RNA from monocytes was extracted with TRIzol reagent (Invitrogen) and purified on (mi)RNeasy Mini Kit columns (Qiagen) at KU Leuven. RNA concentration was determined using the Qubit 2.0 fluorometer (Thermo Fisher Scientific). Average RNA concentration was 122 ng/μl; ranging from 78-382 ng/μl. RNA quality was controlled using the Fragment Analyzer automated capillary electrophoresis system (Advanced Analytical Technologies). Average RQN value was 9.9, indicating very high RNA quality. Libraries for mRNA sequencing were prepared using the TruSeq stranded mRNA sample prep kit (Illumina).