Illumina HiSeq 2500 paired end sequencing
Replicate 7
Genomic DNA was extracted from confluent wells using QIAamp DNA Blood Mini Kit (QIAGEN) and cleaned performing a proteinase K (QIAGEN) digestion step. Genomic DNA (approximately 1 _g) was fragmented to an average size of 150 bp and subjected to DNA library creation using established Illumina paired-end protocols. Adaptor-ligated libraries were amplified and indexed via PCR. A portion of each library was used to create an equimolar pool.