GSM3417239: #7 Tumor-input; Homo sapiens; OTHER
Tissues were homogenized in Trizol reagent with a Tissure-Tearor. Total RNA were extracted using manufacturer's instructions.Poly-adenylated RNA was enriched by using Oligotex® mRNA purification kit (Qiagen). RNA fragmentation, m6A-seq, and library preparation were performed as in Dominissini et al 2013 Nature Protocols. Cell line total RNA was isolated with TRIZOL reagent. Poly-adenylated RNA was enriched by using Oligotex® mRNA purification isolation kit (Qiagen). RNA fragmentation, m6A-seq, and library preparation were performed as in Dominissini et al 2013 Nature Protocols. TruSeq Small RNA Sample Preparation Kit (Illumina)