GSM2719233: media -Fe - RiboZero; Chlamydomonas reinhardtii; RNA-Seq
Total RNA was purified from pelleted cells by freezing in SDS/proteinase K digestion buffer followed extraction with Trizol reagent. ~2 ug total RNA was used as starting material for either rRNA-depletion by RiboZero Plant Leaf kit or for enrichment of poly-A transcripts by binding oligo-T beads (indicated by "library protocol" above). The resulting RNA was then used to generate strand-specific Illumina libraries.