GSM3819737: IGF1_0h_Rep4; Mus musculus; RNA-Seq
RNA was purified with a miRNeasy Micro Kit (Qiagen) supplemented with DNase I (Qiagen). RNA concentration was determined using a NanoDrop 2000 (Thermo Fisher). For each sample, 500ng of RNA was delivered to the University of Michigan Sequencing Core for RNA sequencing analysis. RNA libraries were prepared for sequencing using standard Illumina protocols