GSM2804253: TF rep 2; Fusarium graminearum; RNA-Seq
Fusarium graminearum wild-type strain GZ03639 and three toxoflavin-sensitive mutant strains (∆GzZC190, ∆GzC2H008, ∆GzbZIP005) were grown in 50 ml of complete medium at 25°C with shaking at 200 rpm for 72 h. The filtrated mycelia were incubated in 50 ml of minimal medium with presence of toxoflvin 100 mg l-1 for 24 h at 200 rpm. The mycelia were grinding in liquid nitrogen and total RNAs were extracted with NucleoSpin RNA Plant Kit (Machey-Nagel GmbH & Co. KG) according to the manufacturer’s protocol. RNA-sequencing libraries were prepared using an Illumina TruSeqTM RNA sample prep kit (Illumina), according to the manufacturer’s protocols. Sequencing was performed on an Illumina HiSeq2000 instrument at the National Instrumentation Center for Environmental Management (NICEM).