GSM2399870: Seb1_IP_2_ChIP-Seq; Schizosaccharomyces pombe; ChIP-Seq
Cells were grown to mid-log phase and crosslinked using 11% formaldehyde. Cells were lysed by vortexing with glass beads and chromatin was extracted by two consecutive ultra-centrifugation steps. The DNA was sheared by sonication, Pcf11-TAP was IP-ed using IgG agarose and DNA was extracted by phenol:chloroform. Libraries were generated using the NEBNext fast DNA library Prep set for Ion Torrent (E6270).