GSM4476733: WT Ileum rep 1 (ATAC-seq); Mus musculus; ATAC-seq
Nuclei were exacted in ATAC-Resuspension Buffer (RSB, 10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2) with 0.1% NP40, 0.1% Tween-20, and 0.01% Digitonin. DNA was fragmented by Netera Tn5 Transposase (Illumina) NEBNext 2x MasterMix to pre-Amplify for 5 cycles and determined the required number of additional cycles by qPCR amplification. The final libraries were size selected (200bp to 800bp, including index) with AMpure beads Sequencing strategy was on Illumina HiSeq-2000 Pair-end 150 (PE150)