GSM2719204: At_mesophyll.cell.ATAC_rep2; Arabidopsis thaliana; ATAC-seq
Tn5 Transposase treatment (Using the Illumina Nextera kit) was performed on 25,000 nuclei from each sample, followed by isolation of fragmented genomic DNA using the Qiagen PCR purification kit. Purified transposase-fragmented DNAs were converted to sequencing libraries through a limited number of PCR cycles using indexed primers, followed by purification of library fragments using SPRI beads.