Illumina HiSeq 2500 sequencing; 3prime RNA-seq of human prostate cancer cell line DU-145 treated with Senexin A
3prime RNA-seq of human prostate cancer cell line DU-145 treated with Senexin A
DU-145 cells were treated for 48h with 10µM Senexin A dissolved in DMSO or with equivalent amount of DMSO for untreated controls Total RNA was extracted with Qiagen RNAeasy kit and 500ng total RNA were used as input for the 3' QuantSeq kit library preparation (Lexogen, Vienna, Austria) following the protocol provided by the manufacturer.