hHGF immunized chicken
A total of 4 sets of cDNA including pre-immunization (PI), immunization (IM), boosting (BO) and sacrifice (SC) were amplified using the forward primer (5-ACTCAGCCGTCCTCGGTGTC-3) and reverse primer (5-ACTGACCTAGGACGGTCAGG-3). The chicken genes were then analyzed using an Illumina MiSeq system (Illumina Inc., San Diego, CA, USA) by Celemics, Inc. (Seoul, Republic of Korea). Miseq libraries for DNA sequencing were prepared by SPARK DNA sample prep kit (Enzymatics, Beverly, MA, USA) according to the manufacturers protocol. The final libraries were quantified and qualified using D1000 ScreenTape assays with the Agilient 4200 TapeStation according to the manufacturers protocol. The Miseq reagent kit v3 (Illumina) was used for long paired-end reads (2 x 300 bp).