Expression reads (cDNA) of BRINP1 TRIP pool B, replicate 2
Protocol by Akthar et al. 2014 (Nature protocols) was used to measure barcode expression levels. 2 g of total RNA was reverse transcribed in a 50 l reaction containing 50 ng of oligo(dT) primer and 1 l of Superscript II (Invitrogen). 1 l of cDNA was used as a template for amplification of barcode sequences (two PCR replicates were performed each with a different index). PCR was performed in two rounds and purified products were directly used for high throughput Illumina single-read sequencing.