Illumina MiSeq paired end sequencing; Whole genome sequencing of isolate R0006_KLMC4
Isolate subcultured onto TCBS agar and incubated at 37°C for 24 hours. Colonies from TCBS were used for Genomic DNA extraction. Genomic DNA was extracted using MasterPureTM Complete DNA and RNA Purification Kit (Epicentre, Illumina Inc.). The quality and quantity of genomic DNA were accessed using Qubit 2.0 fluorometer (Invitrogen, Life Technologies). DNA library preparation was done using Nextera XT DNA preparation kit (Illumina Inc.).
Nextera XT