GSM4407038: DVL3_RAD_8; Mus musculus; RNA-Seq
2-4 (10uM) sections were transferred into RNAase free microcentrifuge tube. The paraffin was removed by incubating in xylene and ethanol. For lysate and total RNA purification, digestion buffer and proteinase-K was added to the samples as per manufacturer's instructions (Norgen kit). RNA libraries preparations were generated using the Quant-seq 3' mRNA-Seq FWD kit (Lexogen PART NO k15.96) according to manufacturer's instructions, with 500ng input RNA. FFPE-derived RNA was prepared using the suggested manufacturer's instructions. Libraries were pooled and sequenced on the NextSeq 500 using the FMHLS genomics CTU, yielding an average of 10M reads per sample.