Nuclear-localized STK4 in C4-2 cells
Total RNA in biological replicates was isolated from C4-2/Vector, C4-2/CL-STK4, C4-2/LR-STK4, and C4-2/NL-STK4 cells using RNA isolation kit according to manufacturerŐs instruction (Life Technologies). Quality of total RNA was assessed prior to library construction. Sequencing libraries were prepared using the standard protocol and sequenced by Illumina Genome Analyzer IIx in Genomic Core at Cedar-Sinai Medical Center.