Whole-genome sequencing of Escherichia coli population isolated at day 24  from old mouse A1
The library construction and sequencing were performed at the genomics facility at Instituto Gulbenkian de Ciencia (Portugal). Nextera XT kit from Illumina was used for library construction. Briefly, a sample of mice feces was plated on LB agar and allowed to grow till ~1000 isolated colonies were visible. These colonies were then pooled and DNA was extracted for whole genome sequencing in a MiSeq Benchtop Sequence