qiime_experiment_866:611026:0
Dec deep seq sample illumina
Sample prep and extraction was described in Southward et al. (PMID: 15596166). Illumina sequencing was performed using standard library prep reagents, and v2 clustering and v3 SBS kits. The Illumina paired-end reads were filtered to remove any sequences containing adaptors and then quality filtered during paired end merging, by requiring that at least 10 bases on each end overlapped, and that the overlapping bases were exactly identical. Examination of the quality scores before merging revealed >Q20 across 90% of bases in each read."