NextSeq 550 sequencing; Ribosome profiling and parallel RNA-seq of S. pombe cells subject to amino acid starvation
Ribosome profiling and parallel RNA-seq of S. pombe cells subject to amino acid starvation
Cells were grown at 32C in EMM2 (Moreno et al. Methods Enzymology 194: 795). 30 ng of ribo-depleted RNA was used as starting material for the NEXTflex Rapid Directional qRNA-Seq Kit (Bioo Scientific).