GSM2878083: Mouse erythroid cells biological replicate 1, technical replicate 1; Mus musculus; OTHER
Spleens were gently dissociated into a single cell suspension and cells were passed through a 70 μm strainer to remove clumps. For ter-119+ cell selection, cells were stained with phycoerythrin conjugated anti-ter-119 antibodies and positively selected using anti-phycoerythrin MACS beads before fixation with formaldehyde. Tri-C combines 3C library preparation (NlaIII restriction enzyme) with oligonucleotide capture enrichment that is optimized to detect multiway interactions with viewpoints of interest, as described in detail in the Supplementary Methods of the paper.