GSM2720190: 13571X14 MSN-PEG500-2; Mus musculus; RNA-Seq
Total RNA was extracted and purified following 4 h of incubation with SNPs using Qiagen RNeasy minikit protocol. The samples were kept at −80 °C until further use. RNA was quantified by Nanodrop spectrophotometer ND-1000 and the RNA samples were assessed for quality using an Agilent 2200 TapeStation prior to expression analysis. All RNA samples had RNA Integrity Numbers (RIN) ranging from 8 to 10. Libraries were prepared using Illumina TruSeq RNA Library Prep Kits.  HiSeq2000 50 Cycle Single Read Sequencing was performed with a maximum of 8 multiplexed samples per lane to ensure sufficient depth of sequencing.