GSM3135580: Wild type rep 1; Mus musculus; Bisulfite-Seq
Individual samples [1 term placenta/sample x 3 biological replicates for null and control mice = 6 total samples] were flash frozen prior to DNA isolation and purity assessment Fragments were ligated to pre-annealed adapters containing 5'-methyl-cytosine instead of cytosine Adaptor-ligated size-selected fragments of 150–250 bp and 250–350 bp were bisulfite-treated and sequenced.