Illumina HiSeq 2000 paired end sequencing; Alu element-containing RNAs maintain nucleolar structure and function
Alu element-containing RNAs maintain nucleolar structure and function
Cells were grown in DMEM medium containing 10% FCS, at 37 degree C  and 5% CO2 supply. no treatment RNA was extracted from whole cells or cellular fractions (nucleoplasmic = nuclear minus nucleoli; nucleoli = isolated nucleoli) using the RNeasy Mini Kit from Qiagen. Libraries were prepared using the NEB library prep kit for long RNAs.