GSM3067309: Young_hTERT_biological replicate 4; Homo sapiens; RNA-Seq
Total RNA from NT, EV and hTERT+ cells was isolated using Isolate II RNA mini kit (Bioline) according to manufacturer's protocol. RNA purity and quality was confirmed using a Bioanalyser. RNA-seq libraries were prepared for sequencing on Illumina HiSeq 2500 using the Illumina TruSeq Stranded mRNA sample preparation kit Set A (Illumina, CA, USA). Samples were indexed and run twelve per lane, generating ~ 10 million 50 bp reads per sample.