GSM4155882: TL_4h_R5; Saccharomyces cerevisiae; OTHER
Cells were collected by filtration and then flash frozen in liquid nitrogen. RNA was extracted by AcidPhenol:Chloroform:Isoamyl alcohol isolation. PolyA selection was performed with 100 ug of RNA and oligo-dT DynaBeads. The 5' end sequencing approach was performed as in (Wu et al., 2018). See methods for details.