GSM4310120: MCF-7 rep4_ATAC-seq; Homo sapiens; ATAC-seq
ATAC-seq was performed according to the published protocol (Buenrostro et al., 2013). Briefly, cells were collected in cold PBS and cell membranes were disrupted in cold lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2 and 0.1% IGEPAL CA-630). The nuclei were pelleted and resuspended in the transposase reaction mix (25 µL 2× TD buffer, 2.5 µL transposase (Illumina) and 22.5 µL nuclease-free water). Directly following transposition, the sample was purified using DNA clean & concentratorTM-5 (ZYMO research). Following purification, libraries were amplified using 1× NEBnext PCR master mix and 1.25 µM of custom Nextera PCR primers. AMPure XP beads (Beckman Coulter) were used to purify the transposed DNA and the amplified PCR products. All libraries were sequenced on a 100-cycle paired-end run on an Illumina NOVAseq instrument.