GSM3132553: GM_NOR_A_H3K27me3_ChIPSeq; Mus musculus; ChIP-Seq
RNA was extracted using Trizol, as per the manufacturer's instructions. Histone-DNA complexes for ChIP-Seq were recovered by immunoprecipitation using cell lysates from clarified sonicated nuclei. Libraries were constructed as per the manufacturers instructions. For RNASeq: 500 ng RNA was used with the Illumina stranded total RNASeq protocol; whereas, for ChIP-Seq the Illumina Nextera protocol was used.