GSM4656409: mouse liver Adult; Mus musculus; OTHER
The embryonic or adult mouse liver were washed dissected, homogenized and crosslinked 3 times using 254 nm UV light (0.2 J/cm2 ) in cold PBS. Then the tissue were lysed and incubated with Ago antibodies and sperated by SDS-PAGE and transfer to nitrocellulose.membrane. The RNA were isolated by phenol chloroform extraction RNA were ligated with adapters and reversed tanscipted into cDNA and PCR amplification. HITS-CLIP