GSM4406931: PR0549_34_a; Mus musculus; RNA-Seq
FCM-sorted single crypt epithelial cells were introduced into the cell input well of the C1 Single-Cell Auto Prep System (5–10 µm). Single cells captured on the IFC were microscopically inspected with a Keyence BZ-X700 to determine which C1 capture sites contained only a single cell. Reverse transcription and cDNA preamplification were performed using the SMARTer Ultra Low RNA kit (Clontech). Sequencing libraries were prepared using the Nextera XT DNA Sample Preparation Kit and the Nextera Index Kit (Illumina), according to the manufacturer's instructions. The preparation and sequencing of cDNA libraries and the extraction of multiple expression data were performed by Takara Bio, Inc.