GSM4039013: sh-KDM10A/KDM10B; Homo sapiens; RNA-Seq
HeLa cells were lysated by TRIzol (Invitrogen),then column-purified with Direct-zol RNA MiniPrep Kit (Zymo research) to obtain total RNA.The RNA samples were tested with Agilent 2100/agarose electrophoresis. RNA libraries were prepared using standard Illumina protocols. 1.RNA was purified with Ampli-oligT,then reversely transcripted with SMART cDNA Library Construction Kit to get the first strand of cDNA; 2.The DNA was amplified and purified with Ampure xp beads agencourt; 3.Fragmentation, purification, and amplification of DNA with Thermal cyclerS1000 (Bio-Rad); 4.Test the DNA with agarose electrophoresis to obtain a 250bp-350bp main band, then purified with Ampure xp beads agencourt; 5.Test the quanlity of libraries with StepOne Plus qPCR (ABI).