GSM2546539: Neural progenitor cells duplicate 1; Mus musculus; RNA-Seq
Heterozygous Nestin-GFP mice were bred with homozygous DCX-mRFP mice to yield GFP/RFP double positive embryos. The cortices derived from the double positive embryos were dissociated by trituration in HBSS with 2mM of EDTA and 25 ug/ml of DNase I. Cells were washed once with HBSS and re-suspended in DMEM/F12. Fluorescence-activated cell sorting (FACS) was used to acquire neural progenitor cells (NPCs, green color) and daughtor neurons (double color). Total RNA was extracted using TRIzol. Following Illumina's True-seq RNA-seq library prep kit