GSM3208723: Donor 2; Homo sapiens; RNA-Seq
Total RNA was isolated with Trizol (Invitrogen) and quantified using a Nanodrop Spectrophotometer (ThermoFisher Scientific, Germany) RNA sequencing (RNA-seq) of nuclear extracts from Ctrl and IPF primary fibroblasts was performed by Single End (SE) sequencing using Nextseq500 (Illumina) sequencer with V2 chemistry. RiboGone™ - Mammalian (Clontech) was used to deplete ribosomal RNA from the samples. SMARTer® Universal Low Input RNA Kit for Sequencing (Clontech) was used for pre-amplification before library preparation. Library preparation was performed using the Low Input Libray Prep Kit (Clontech) following the manufacturer's instructions.