GSM3729199: Cancer_5; Mus musculus; RNA-Seq
Indicated tissue were collected, washed by sterile PBS twice, and RNA was extracted using mirVana miRNA Isolation Kit (ThermoFisher, AM1560). Library construction protocol: Directional RNA-seq libraries were prepared from 800ng total RNA using the NEBNext Directional Ultra RNA Library Prep Kit for Illumina (New England Biolabs), with initial polyA+ isolation.