GSM2977868: ICCV2 Genotype Stress 2 Reproductive stage; Cicer arietinum; RNA-Seq
RNA was extracted using Qiagen RNeasy mini kit The sequencing for root samples was performed by Ion-Proton PGM sequencer. Four samples were pooled together in equimolars on a sequencing chip to to generate single end reads. The raw Fasta files generated by sequencing were obtained and were cropped and trimmed using q-trim command line tool to remove low-quality reads and adapter sequences. Followed by Alignment and differential expression studies.