GSM4656397: Expt2: G7_4; Mus musculus; RNA-Seq
Total RNA was extracted according to QIAzol lysis based reagent  miRNA Mini Kit ( Qiagen Inc, Germantown, MD, USA). Qualitative and quantitative analysis of extracted RNA samples were carried out with a 2100 Bioanalyzer Nano Chip (Agilent Technologies Genomics, Santa Clara, CA USA). RNA Integrity Number (RIN) of extracted RNA samples applied for RNA Seq ranged between the values 8.4-9.4 and RNA samples were stored at -80°C. Strand-specific mRNA sequencing libraries were constructed from 100 ng of total RNA with the TruSeq Stranded mRNA Library Prep Kit (Illumina).