GSM4474541: RNA_Macrophages_HO1-KO3; Mus musculus; RNA-Seq
TAMs were isolated by FACS in RLT buffer and flash frozen. RNA extraction was performed using RNeasy kit (Qiagen) and sample quality was tested on a 2100 Bioanalyzer (Agilent) Libraries were prepared using Ovation® SoLo RNA-Seq system (NuGEN Technologies) and underwent paired-end sequencing (25×106 paired-end reads/sample, Novaseq 6000 platform)