GSM2878165: qteE log-phase replicate 1; Pseudomonas aeruginosa; RNA-Seq
mRNA was preserved immediately upon cell harvest using Qiagen RNAprotect. Cells were lysed using sonication and mRNA was extracted using Qiagen RNEasy miniprep columns, followed by DNAse I treatment and cleanup using another round of RNEasy-based purification. rRNA was removed using Illumina RiboZero protocol. 100bp PE Illumina TruSeq using Wafergen Apollo protocol Sequencing performed on Illumina HiSeq 3000, 30 samples in 1 lane