GSM4155868: RNA_4h_r5; Saccharomyces cerevisiae; RNA-Seq
Cells were collected by filtration and then flash frozen in liquid nitrogen. RNA was extracted by AcidPhenol:Chloroform:Isoamyl alcohol isolation. PolyA selection was performed with 100 ug of RNA and oligo-dT DynaBeads. The RNA-seq libraries were prepared using the NEXTflexTM Rapid Directional mRNA-Seq Kit (Bioo Scientific) according to manufacturer's instructions. 100 ng of poly-A selected mRNA was used for all libraries. AMPure XP beads (A63881, Beckman Coulter) were used to select fragments between 200-500 bp.