cDNA sequencing of genes expressed during limb regeneration
cDNA libraries were generated for Day 0, NR5, NR14, DL5, and DL14 RNA samples using the Super SMART cDNA Synthesis protocol (Clontech, Mountain View, CA). Single stranded cDNA template was amplified using the Advantage 2 PCR Kit (Clontech) and size selected according to manufacturer’s instructions.