GSM2454326: SIPS_3; Homo sapiens; RNA-Seq
Total RNA was isolated with TRIreagent (Sigma) following the recommended protocol. RNA concentration and quality was analysed with a Bioanalyzer 2100 using the RNA 6000 Nano Kit (Agilent Technologies) before the samples were sent for next generation sequencing. RNA libraries were prepared for sequencing on HiSeq 2500 (Illumina) using standard Illumina protocols by GATC BIOTECH (Konstanz, Germany)