SOLiD 3 Sequencing of S. paradoxus 3395
RNA was extracted using the TRIzol pro- tocol (Invitrogen Part #15596026), and ribosomal RNA was depleted using the RiboMinus Eukaryote Kit for RNA-seq (Invitrogen Part #100004590). The SOLiD RNA-seq library construction protocol was used (Life Technologies Part #4452437), with the following modification: The size-selection step was extended to include sequencing templates containing RNA fragments as small as 50 nucleotides.