RNAseq of Macrobrachium rosenbergii (giant freshwater prawn)
The cDNA library was prepared using SENSE mRNA-Seq Library Prep Kit V2 (Lexogen, USA) and the purification module with magnetic beads (Lexogen, USA). Each library was indexed by 6-nucleotide-long i7 indices during library amplification step according to manufacturers protocol. The prepared cDNA library was subjected to quality assessment using Qubit 4 Fluorometer (Invitrogen, USA) and LabChip GX Touch 24 microfluidic nucleic acid analyzer (PerkinElmer, USA). Before denaturation, equal amount of each cDNA library were pooled together and re-purified using the magnetic beads. Total of 2 nM pooled library was subjected to denaturation steps according to NextSeq System Denature and Dilute Libraries Guide (Illumina, USA). Cluster generation and paired-end sequencing with 75 bp were performed on a NextSeq 550 sequencer (Illumina, USA) using NextSeq 500/550 High Output Reagent Cartridge v2 150 cycles (Illumina, USA).