GSM3302803: H2D rep1 (for LncRNA-seq); Mus musculus; RNA-Seq
Total RNA from liver samples was extracted individually using RNAiso Plus (Takara Biotechnology, Dalian, China). An equal amount of RNA (n = 4-8) from the same group was combined into 2 samples/group for lncRNA sequencing and 1 sample/group for small RNA sequencing as described previously (Ding et al., 2014). Sequencing was performed by Novogene (Beijing, China). For lncRNA sequencing, briefly, ribosomal RNA was removed by Ribo-zero rRNA Removal Kit (Epicentre, USA), and sequencing libraries were generated. The clustering of the index-coded samples was performed using TruSeq PE Cluster Kit v3-cBot-HS (Illumina). After cluster generation, the libraries were sequenced on an Illumina Hiseq 2500 platform and 125 bp paired-end reads were generated. For small RNA sequencing, briefly, sequencing libraries were generated using NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB, USA) and index codes were added to attribute sequences to each sample. The clustering of the index-coded samples was performed, after which the library preparations were sequenced on an Illumina Hiseq 2500/2000 platform and 50bp single-end reads were generated. lncRNA RNA-Seq and small RNA RNA-seq