454 sequencing of Papio anubis placenta transcript fragment library
     Baboon placental tissue was provided by the Southwest National Primate Research      Center (SNPRC). Total RNA was isolated using the TRIZOL method followed by mRNA      extraction using Poly(A) Purist kit (Ambion). mRNA was sent to Evrogen for cDNA      library creation. In short, full length cDNA was generated using SMART protocol      (16 PCR cycles), normalized via Double Strand Nuclease (DSN) (18 PCR cycles),      cloned into the pAL 17.3 vector and transformed into E. coli.  We received the      cloned normalized library as well as the non-cloned normalized library.         