GSM2544938: LightBlue558V; Taeniopygia guttata; RNA-Seq
Total RNA was extracted from Area X or ventral striatopallidum tissue micropunches using Qiagen Rnease Micro Kits following the manufacturers protocol. We used QIAzol as the lysis reagent. We also performed an additional wash each in RW1 and RPE buffers beyond the manufacturers protocol. Total RNA extracts were provided to the UCLA Neurogenomics Core. PolyA transcripts were selected for as part of the library construction protocol. Librarires were prepared using the Illumina TruSeq Poly-A prep kit using the manufacturer's protocol.