Transcriptome analysis on drought-sensitive cultivar of Musa spp
Transcriptome library for sequencing was constructed according to the         Illumina TruSeq RNA library protocol outlined in ?TruSeq RNA Sample Preparation Guide? (Part         # 15008136; Rev. A; Nov 2010). Briefly, 1 lg of Total RNA by qubit was enriched for Poly-A         using RNA Purification Beads provided with the kit, enriched RNA was fragmented for 4         minutes at elevated temperature (94oC) in the presence of divalent cations and reverse         transcribed with Superscript II Reverse transcriptase by priming with Random Hexamers.         Second strand cDNA was synthesized in the presence of DNA Polymerase I and RnaseH. The cDNA         was cleaned up using Agencourt Ampure XP SPRI beads (Beckman Coulter). Illumina Adapters         were ligated to the cDNA molecules after end repair and addition of ?A?- base. SPRI cleanup         was performed after ligation. The library was amplified using 16 cycles of PCR for         enrichment of adapter ligated fragments. The prepared library was quantified using Nanodrop         and validated for quality by running an aliquot on High Sensitivity Bioanalyzer Chip         (Agilent). The prepared library was sequenced using Illumina Hiseq 2000