GSM3417510: Bcell_DARTseq_0; Homo sapiens; RNA-Seq
The following steps are identical to those performed using standard Drop-seq protocol (Macasko, 2015). Briefly, droplets containing beads and lysed cells were broken and washed. Complementary DNA was created using Maxima Reverse Transcriptase (Thermo Fisher) and a transcript switch oligomer. cDNA was amplified using 15 cycles of PCR. Amplified cDNA libraried were tagmented and amplified using Nextera. Illumina sequencing was performed on the libaries using 20x130 paired-end sequencing.