GSM4474605: Transplant; Danio rerio; RNA-Seq
Single cell was partitioned through Chromium platform (10X Genomic) which encapsulate each cell in individual GEMs. The cell lysis, polyA pull down, tagging with cell barcode, and RNA:DNA hybrid synthesis were performed in the GEMs. The version 2 of Chromium Single Cell 3' Reagent Kits was used for this RNA extraction step. After cell-barcoding the extracted RNA, the GEMs were lysis for further fragmentation and ligation of illumina P5 and P7 ends with the version 2 of Chromium Single Cell 3' Reagent Kits. Two size selections were performed for long fragments (> 1000 bps) and short fragments (< 1000 bps) before fragmentation and after finalization respectively.