GSM4041272: MPs5vm.1; Danio rerio; OTHER
The contents of the separated intestine are extruded. Total DNA in the intestinal contents was isolated with DNeasy Blood & Tissue Kit (QIAGEN,Germany). DNA concentration was measured using a NanoDrop spectrophotometer (NanoDrop One,Thermo, USA). After DNA quality analysis by electrophoresis, the extracted DNA was amplified using primers(Forward primer:5'-CCTAYGGGRBGCASCAG-3'; Reverse primer: 5'-GGACTACNNGGGTATCTAAT-3') to target the V3-V4 regions of the bacterial 16S rRNA gene. The obtained PCR products were purified by GeneJET Gel Extraction Kit (Thermofisher, USA) and applied to construct the sequencing library. The library was sequenced on an Ion S5TM XL platform.