Microbial diversity analysis of a marine sediment core
Gemonic DNA was extracted from 14 sediment subsamples. Primers 515F/806R were used to amplify the V4 region of the 16S rRNA genes, with bar code sequences on the primers. PCR was conducted in triplicate, and the products were pooled for each subsample. A composite DNA sample for sequencing was created by combining equimolar ratios of amplification products.