GSM4258346: T_M2_S13: E18.5 trachea RNAseq; Mus musculus; RNA-Seq
Control and MyocdCKO E13.5 lungs, E18.5 tracheas, and E18.5 lungs were dissected in PBS and immediately stored in TRIzol Reagent (ThermoFisher Scientific). Tissues were then lysed using a TissueLyser (Qiagen), and total mRNA was extracted using standard TRIzol RNA extraction protocol. mRNA was cleaned using the RNeasy Micro Kit (Qiagen). cDNA libraries were made for each individual lung or trachea using Illumina TruSeq RNA Library Prep Kit V2 (Illumina) and sequenced on a SR75 run on the HiSeq4000 platform (Illumina).