GSM2292522: hnRNPA2/B1_iCLIP_001; Mus musculus; RIP-Seq
Spinal cords were rapidly extracted by hydro-extrusion and snap frozen in liquid nitrogen. Frozen samples were finely ground and UV crosslinked before lysis and immunoprecipitation. 8 week old, female, C57/BL6J mice were obtained from Jackson Labs (Bar Harbor, ME). Mice were sacrificed and the spinal cords were removed, flash frozen in liquid nitrogen, and ground finely with a mortar and pestle. The frozen cords were UV crosslinked at λ=258nm with 4000 mJ/cm2 of energy. iCLIP libraries were prepared as previously described (Huppertz et al. 2014) using rabbit polyclonal antibody GTX127928 (Genetex) (10 mg per sample). Libraries were sequenced on the HISEQ 2500 platform in single end mode with a read length of 50 base pairs.