A Transcriptome Sequencing  (TSEQ) protocol using random primed cDNA amplification
Poly-A  RNA was prepared from 50 *g total RNA by two rounds of purification with oligo(dT) magnetic beads (PureBiotech, Middlesex, NJ) to minimize ribosomal RNA contamination.  Poly-A  RNA quality was assessed by Agilent Bioanalyzer RNA 6000 Nano assay (Agilent Technologies, Inc., Santa Clara, CA) and quantified by Quant-iT RiboGreen assay (Invitrogen, Carlsbad, CA).