GSM4476092: Scgb1a1 KO, 4 wk, replicate 2; Mus musculus; RNA-Seq
Alveolar macrophages were purified from bronchoalveolar lavage cells after staining with antibodies and sorting by flow-cytometry. Purified alveolar macrophages were immediately lysed in RNA Lysis buffer and total RNA was extracted. Libraries were prepared according to Illumina's sequencing workflow. Briefly, the total RNA was treated with DNase I and poly-A library was prepared following Cofactor Genomics' picoRNA workflow (CFG003-60).