wt-1
Indexed libraries were constructed from 1000 ng of total RNA using the TruSeq Stranded mRNA Sample Prep Kit (Illumina) following the manufacturer's instruction. The quantity and quality of the libraries were also assessed by Qubit and Agilent 2100 Bioanalyzer, respectively. The average library size was 400 bp. Libraries molar concentration were validated by qPCR for library pooling.