GSM3728455: Cervical_2; Mus musculus; RNA-Seq
For each sample, 50-120 labelled cells were isolated by manual cell sorting on a fluorescence dissecting scope. Following pooling and lysis, total RNA was extracted by Picopure kit (KIT0204; Thermo-Fisher) Amplified DNA was produced using Ovation RNA-seq System V2 kit (#7102; NuGEN), fragmented to ~200bp, ligated to Illumina sequencing adaptors with Encore Rapid kit (#0314; NuGEN), and sequenced on an Illumina HiSeq 2500 with 4-fold multiplexing (single end, 100bp read length)