GSM3463833: Salmonella_lumen rep1; Microbiota; RNA-Seq
Nucleic acids were extracted using a phenol:chloroform extraction method and treated with DNase I (Thermo Scientific) until DNA free. RNA was cleaned up and concentrated using the GeneJET RNA cleanup kit and Concentration Micro Kit (Thermo Scientific). rRNA was removed using the Ribo-Zero bacterial kit (Illumina, San Diego, USA) RNA libraries were prepared for sequencing using standard Illumina protocols