Normalization reads (plasmid DNA) of plasmid mix with TRIP promoter constructs
For ADAMTS1, ARHGEF9, BRINP1, MED30, PGK, TMEM106B and ZNF300  promoters, barcoded TRIP plasmid library was transformed into DH5-alpha E. coli and plated; 100 colonies were picked, pooled, and expanded to produce plasmid mini-libraries with 100 different barcodes each. These mini libraries were mixed together and sequenced to measure plasmid abundance. Plasmid abundance was used to normalize barcode expression.