GSM2977234: Adult-Isoseq; Xenopus tropicalis; RNA-Seq
Iso-seq libraries were constructed by the Genomics Core Laboratory, Washington State University. The Iso-seq method includes several major steps: poly(A)+ RNA enrichment, full length cDNA synthesis, duplex-specific nuclease normalization, PCR amplification, size selection. Three fractions of cDNA, 1-2kb, 2-3kb, 3+kb were collected and sequenced. Total RNA was harvested using Trizol reagent.