GSM3211195: Subject19_mucosa_CD69+; Homo sapiens; RNA-Seq
Full-length cDNA was prepared from 250-1000 sorted CD4+CCR5+ cells using SMARTer Ultra Low Input RNA Kit for Sequencing - v4 according to manufacturer's instructions (Clontech, Mountain View, CA). Libraries were prepared from amplified cDNA using the NexteraXT kit according to manufacturer's instructions (Illumina, San Diego, CA).