GSM2804112: iPS Input; Homo sapiens; ChIP-Seq
On Day 8, Cells were fixed with 1% PFA for 10 min, quenched with glycine, collected and frozen at -80C.  Cell pellets were lysed (85mM KCl; 5mM PIPES;0.5% NP40), nuclei were collected and chromatin was sonicated on a S220 Focused-ultrasonicator (Covaris) to a mean size of 300-500 bp.