Swanson_450bp_260nt
The shotgun library was prepared with the Hyper Kapa Library Preparation kit (Kapa Biosystems). Library insert size was 350bp - 500bp (average 450bp). The library was quantitated by qPCR and sequenced on four lanes of a HiSeq2500 for 261 cycles from each end of the fragments using a Rapid TruSeq SBS kit version 2. Fastq files were generated and demultiplexed with the bcl2fastq v1.8.4 Conversion Software (Illumina).