GSM2977043: Sample_shZc3h13_nucleus_m6A_Input_rep2; Mus musculus; OTHER
Total RNAs from mESCs were isolated using Trizol reagent according to the manufacturer's instruction (Life technology, 15596018). Polyadenylated RNAs were extracted by oligodT beads (NEB, S1419S), followed by removal of contaminated rRNA with RiboMinus transcriptome isolation kit (Life technologies, A15026). RNA libraries were prepared for sequencing using standard Illumina protocols