GSM3463522: Control Rep. 3 (Esr1fl/fl); Mus musculus; RNA-Seq
The CNS was rapidly dissected and a 2 mm coronal section containing the hypothalamus was isolated. The arcuate nucleus was manually microdissected from the tissue block, and RNA was prepared using the Invitrogen Pure Link RNA Mini kit (Cat#12183018A). The Nugen Ovation RNA-Seq System V2 Sample Prep Kit (Cat#7102) was used with 50 ng of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using manufacturers protocol.