GSM2722106: ATAC_miNs_D20_A; Homo sapiens; ATAC-seq
DNA fragments were collected by column-based purification after tagmentation. ATAC-seq was performed as previously described (Buenrostro et al., 2013). Briefly, 50,000 cells were collected for ATAC-seq library preparation at Ctrl day 10, miNs day 10, miNs day 20, shBRG1 Day20 and shEGFP day 20. Transposition reaction was carried out with Nextera Tn5 Transposase for 30 min at 37 °C. Library fragments were amplified for optimal amplification condition. Final libraries were purified using AMPure XP beads (Ampure) and sequenced with 50 bp paired-end reads on Illumina HiSeq 2500 or 75 bp paired-end reads on Illumina NextSeq 500.