GSM4155765: VP64_TIL_2; Mus musculus; RNA-Seq
2 µg of total RNA underwent quality control (Bioanalyzer; all RIN values > 8.0), and was prepared for directional, PolyA+ RNA sequencing at Hudson Alpha using NEBNext reagents (New England Biolabs) according to manufacturer's recommendations with minor modifications (including the use of custom library adapters and indexes). An AllPrep DNA/RNA Mini Kit was used for experiments requiring genomic DNA and total RNA from the sample.