GSM2546322: 6 hpi; Strongylocentrotus purpuratus; RNA-Seq
Larvae were collected using 100 uM Nitex mesh, washed three times in ASW, pelleted by centrifugation and stored in Trizol.  Whole RNA was extracted from Trizol preparations using manufacturers instructions. Polyadenylated RNA was purified using the Poly(A)Purist Kit (Ambion). Libraries were prepared for SOLiD sequencing using standard ABI protocols.