Bacterial Diversity Analysis:intestinal bacteria of C57 / BL6 mice
The DNA samples were subjected to PCR amplification with primers 515F (5-GTG CCA GCM GCC GCG GTA A-3) and 907R (5- CCG TCA ATT CMT TTR AGT TT-3) targeting the V4-V5 hypervariable region of the 16S rRNA gene, and with primers ITS3F (5- J. Wang, et al. Environment International 138 (2020) 105600 3GCA TCG ATG AAG AAC GCA GC-3) and ITS4R (5-TCC GCT TAT TGA TAT GC-3) targeting the ITS2 region of the ITS gene.