GSM3728349: pUC57 AIRN G3; Escherichia coli; Bisulfite-Seq
The plasmids were purified using the NucleoBond® XtraMidi kit (Macherey-Nagel) according to the manufacturer's recommendations. DNA was transcribed in vitro using T3 RNA polymerase and was then treated with sodium bisulfite at non-denaturing condition using Zymo Lightning bisulfite modification kit. Library was constructed using Pacific Biosciences protocol.