454 sequencing of Sencecio madagascariensis transcriptome fragment library
Two ug of mRNA was converted to double stranded DNA using the Clontech  SMART cDNA synthesis kit. cDNA amplified from Senecio madagascariensis was normalized according to the protocol supplied with the Evrogen Trimmer Direct cDNA Normalization Kit. Two subtractive libraries were generated using a modified version of the Clontech PCR-Select™ cDNA Subtraction Kit.