Methylobacterium extorquens PA1 CM2730
Genomic DNA was extracted using the Epicentre Masterpure Complete DNA and RNA Purification Kit, following the manufacturer protocol for DNA purification, and DNA from three populations (M. extorquens selected by growth on formaldehyde) were pooled. Genomic DNA was used to make shotgun libraries using the TruSeq PCR-free Library Prep Kit with the HiSeq-length insert option (short), amplified using KAPA, cleaned using MagBio magnetic beads, and quantified using fluorometry. Sequencing was conducted in a 1x100 run on a HiSeq 4000 at the University of Oregon Genomics and Cell Characterization Core Facility; reads were demultiplexed by the facility using bcl2fastq Conversion Software.