DNA-SeqScaffold of Thraustochytriidae sp. SZU445:fungus
The genomic sequence was interrupted by ultrasound, the end of the fragment was repaired, PolyA was added to the 3' end of the fragment, the linker was ligated at both ends, PCR amplified and cyclized, and the single-stranded circular DNA molecule was replicated by rolling circle to form a DNA containing more than 300 copies. Nanospheres, combined with probe anchor polymerization (cPAS) for sequencing