WGS of Diutina catenulata
Soil sample was taken from canal bank in Dublin, Ireland and inoculated in 9 ml YPD (1% yeast extract, 2% peptone, 2% dextrose) containing 0.03 mg/ml chloramphenicol and 0.1 mg/ml ampicillin. Culture was incubated at 30 C, shaking, for 48 h. 10  l was subcultured to fresh media for 24 h. Genomic DNA was extracted by the phenol-chloroform method. Paired-end libraries were prepared using a custom Nextera-based protocol by staff at the Earlham Institute, Norwich, UK. Libraries were subsequently sequenced on two lanes of an Illumina HiSeq 2500.