GSM3416967: Non Aerated Bio Rep1; Arabidopsis thaliana; RNA-Seq
Plant were harvested 15 days after vernalization, freeze in liquid nitrogen, grind in (LN2) cold mortar and total RNA was isolated using plant RNA-easy kit (IBI Scientific, USA). Total RNA was checked for quality using Bioanalyzer (Bio-Rad) RNA sequencing libraries were prepared using standard unstranded RNA Sample Prep Kits (Illumina Inc., San Diego, CA) and samples sequenced single end 100 bps sequencing on an Illumina HiSeq 3000 instrument at the Iowa State DNA facility