GSM3417932: HEK293T_GFP; Homo sapiens; RNA-Seq
Total RNA was extracted with Total RNA Purification Plus Kit (Norgen Biotek Corp.) with DNase I treatment, according to the manufacturer's instructions. Quality control was performed with Agilent Bioanalyzer and RNA quantification with Nanodrop and Qubit. Illumina TruSeq strand-specific RNA libraries (Ribosomal depletion using RiboZero human) containing 125 bp pair-end reads were obtained for each sample on a HiSeq2000 sequencer (Illumina) using standard Illumina protocols.