GSM3133038: g7; Homo sapiens; RNA-Seq
RNA from cells was extracted using Trizol reagent (Sigma). All RNA samples were detected and analyzed in Vazyme Biotech Co. (Nanjing, China). Briefly, RNA purity was detected by NanoDrop® spectrophotometer (Thermo Fisher, MA, USA), concentration measured using Qubit® RNA Assay Kit in Qubit® 3.0 Fluorometer (Life Technologies, CA, USA), and the integrity assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). The sequencing libraries were generated using the VAHTS mRNA-seq v2 Library Prep Kit for Illumina® (Vazyme, NR601) following manufacturer's instructions and sequenced on an Illumina Hiseq X Ten platform using 150 bp paired-end module.