GSM3589586: E2_16_rep3 [E_16_3]; Danio rerio; RNA-Seq
We extracted the total RNA using the Trizol method as described in GSE123439. We assessed the overall quality of extracted RNA by GEL, measured its purity with NanoPhotometer spectrophotometer (IMPLEN, CA, USA), quantified its concentration with Qubit 3.0 Flurometer (Life Technologies, CA, USA), and checked its integrity by Agilent 2100 bioanalyzer (Agilent Technologies, CA, USA). Stranded PolyA+ RNA libraries were prepared at Anoroad (Beijing, China) with in-house kits. A total of 56 qualified cDNA libraries were constructed and were sequenced on the Illumina HiSeq XTen System (Illumina Inc., San Diego, CA).