RNA-seq of Pagrus pagrus male gonad
mRNA paired-end library construction with the Illumina TruSeqTM RNA Sample Preparation Kits v2 following the manufacturerŐs protocol (Poly-A mRNA isolation with oligo-dT beads, mRNA fragmentation, followed by transcription into first-strand cDNA using reverse transcriptase and random hexamer primers) and sequenced as 100bp paired reads