Illumina HiSeq 1000 sequencing; Transcription profiling of cytoplasmic and nuclear RNA of hnRNPC depleted cells and ER-Raf1 activated HR1 cells
Transcription profiling of cytoplasmic and nuclear RNA of hnRNPC depleted cells and ER-Raf1 activated HR1 cells
HR1 cells were reverse-transfected with 5nM siRNA targeting hnRNPC mRNA (HSS179304 and HSS179305) or control siRNA Negative Control with medium GC content (Invitrogen). Depletion of hnRNPC was achieved 48 hours after one siRNA transfection. RNA was extracted using trizol as per manufacturer instructions. RNAseq libraries were produced using TruSeq unstranded RNAseq library kit (Illumina).