GSM4476676: miNC RNA-seq replicate 1; Homo sapiens; RNA-Seq
Total RNA was isolated using TRIzol (Invitrogen). A172 cells were transfected with control miRNA or miR-324 duplexes using the Lipofectamine 3000 reagent (Thermo Scientific). Cells were harvested after 1 day of transfection and total RNAs were isolated using TRIzol (Invitrogen). The RNA quality was confirmed using Agilent 2100 Bioanalyzer and DNA nanoball sequencing for transcriptome (oligo dT enrichment; stranded; 100 bp paired-end) was performed on the BGISEQ-500 platform by BGI Tech Solutions (Hong Kong).