GSM3415990: WT pool (circRNA); Mus musculus; ncRNA-Seq
Hippocampi were fast isolated and flash frozen in liquid nitrogen. Total RNA was extracted using Trizol reagent. Sequencing libraries were generated using the NEBNext® UltraTM RNA Library Prep Kit for Illumina (NEB, USA).Three independent libraries were constructed for miRNA, lncRNA and circRNA sequencing respectively. Specifically, for miRNA sequencing: 5'-adaptor and 3'-adaptor were first added to the total RNA, and a cDNA sub-library with insert of 18-40 nt was isolated by agarose electrophoresis and subject to sequencing; for lncRNA sequencing, ribosomal RNA was removed prior to the library construction by Epicentre Ribo-zero rRNA Removal Kit (Illumina, USA); for circRNA, linear RNA was removed from the ribosomal RNA-depleted RNA by using RNase R before establishing the sequencing libraray.