GSM2721936: E2166-Y-F-E4-T; Mus musculus; RNA-Seq
Fourteen days post-injury, mice were anesthetized using Avertin (250 mg/kg of body weight, i.p.) and perfused transcardially with 20 ml of cold 0.1 M PBS Brains were rapidly removed and a 1.5 mm coronal section of the brain, including the injury site, was taken by slicing the brain at − 2.5 mm and − 4.0 from bregma Within the coronal slice, the hemispheres were separated, and the subcortical tissue was dissected out; hippocampal and cortical tissue were snap-frozen together for mRNA-seq RNA was isolated from frozen cortices and hippocampi at the injury site and purified using RNeasy kit (Qiagen) according to the manufacturer recommendations Libraries were generated by PCR enrichment including incorporation of barcodes to enable multiplexing