Update of Spiroplasma poulsonii MSRO genome
Spiroplasma DNA was extracted from fly hemolymph as previously described (Paredes et al. 2015) . Processing of thesamples was performed in the University of Lausanne Genomic Technologies Facility. The DNA wassheared in a Covaris g-TUBE (Covaris, Woburn, MA, USA) to obtain 20 kb fragments. After shearingthe DNA size distribution was checked on a Fragment Analyzer (Advanced Analytical Technologies,Ames, IA, USA). 5 µg of the sheared DNA was used to prepare a SMRTbell library with the PacBioSMRTbell Template Prep Kit 1 (Pacific Biosciences, Menlo Park, CA, USA) according to themanufacturer&#39;s recommendations. The resulting library was size selected on a BluePippin system(Sage Science, Inc. Beverly, MA, USA) for molecules larger than 20 kb. The recovered library wassequenced on one SMRT cell with P6/C4 chemistry and MagBeads on a PacBio RSII system (PacificBiosciences, Menlo Park, CA, USA) at 240 min movie length.