GSM2719715: glp1_D12_rep2_ribo-RNASeq; Caenorhabditis elegans; OTHER
For ribo-RNAseq, the libraries were prepared by Ovation Human FFPE RNA-Seq Library Systems from NuGEN. Worms at L3,adult day 2, and adult day 12 stages were washed with ice cold M9 three times.  Worm pellets were stored at -80°C before chromatin immunoprecipitation and RNA extraction. For ChIP-seq, chromatin immunoprecipitation was performed as described (Ercan et al. 2007; Landt et al. 2012).  Worm pellet was grinded with mortar/pestle and cross-linked with 1% formaldehyde in PBS at room temperature for 10 min.  Worm fragments were collected by spinning at 3,000 g for 5 min and resuspended in FA buffer followed by sonication with Bioruptor. For ribo-RNAseq, total RNA was extracted from worms harvested at the same stages at adult day 2 and day 12 as ChIP-seq sample preparation.