GSM4476787: KO Colon  (ScRNAseq); Mus musculus; RNA-Seq
Cells washed with 0.4% BSA in PBS and processed with 10X genomics single cell droplet sample preparation workflow at the Genomics Core facility at Weill Cornell Medicine Beads-in-Emulsion (GEMs) were transferred and GEMs-RT was undertaken in droplets by PCR incubation. After purification of first-strand cDNA from the post GEM-RT reaction mixture, barcoded and full-length cDNAs were amplified via PCR to for library construction. Enzymatic fragmentation and size selection were used to optimize the cDNA amplicon size. TruSeq Read 1 (read 1 primer sequence) was added during GEM incubation.  A sample index and TruSeq Read 2 (read 2 primer sequence) were added via End Repair, A-tailing, Adaptor Ligation, and PCR