WGS of Angiostrongylus cantonensis: Canary Islands
DNA was isolated from dried ca 0.5 cm segment of the mid-body of a single nematode using the Nucleospin Tissue XS (Macherey-Nagel, Dren, Germany) according to manufacturers instructions and eluted in 100 l of Tris buffer (pH = 8.5). DNA was stored at -20 C. Isolated DNA was used for Illumina Nextera XT library construction followed by the next generation sequencing using 100bp paired end Illumina HiSeq 2500 sequencing systems utilizing at the depth of 1Gb of raw sequence data (Macrogen, Seoul, Republic of Korea).