Illumina HiSeq 4000 paired end sequencing; Transcriptomic analysis of mouse splenic B cells that are either TG2-sufficient or TG2-deficient
Transcriptomic analysis of mouse splenic B cells that are either TG2-sufficient or TG2-deficient
Splenic B cells were collected from TG2-sufficient and TG2-deficient mouse under resting and stimulated (2 µg / ml murine TG2 for 120 min) conditions. Total RNA was isolated from B cells with an RNeasy minikit (Qiagen). RNA integrity was analyzed on Agilent Bioanalyzer using Agilent RNA 6000 Pico kit. RNA integrity was 9.1 ± 0.7.  RNA-seq libraries were generated from purified rRNA-depleted RNA samples using the strand-specific TruSeq stranded mRNA library prep kit (Illumina, San Diego, CA, USA).