GSM4476316: Crb2 S267A- 4woDox (control, rep4); Homo sapiens; RNA-Seq
RNA was isolated from cell lines using GenElute™ Mammalian Total RNA Miniprep Kit (Sigma-Aldrich). NEBNext Ultra Directional RNA II Library Prep kit was used for the construction of sequencing libraries, which were then sequenced with the Illumina NextSeq 500 System and NextSeq v2.5 sequencing reagent kit in a single read-mode with a read-length of 75 bp.