GSM2367486: MCF-7 4SU UV, no IR replicate 2; Homo sapiens; RNA-Seq
RNA was extracted by adding Trizol LS (Thermo Fisher) and following the miRNAeasy kit (Qiagen) protocol. Next, 2 µg of total RNA were treated with 0.4 U Turbo DNase (Thermo Fisher) for 30 min at 37 ºC, phenol-chlorotorm extracted, ethanol-precipitated and resuspended in water. Equal amounts of total RNA (1 µg) were adjusted to 50 µl final volume with water, and input into the Truseq unstranded mRNA kit v2 (Illumina) using 2 rounds of oligo-dT enrichment. Manufacturer's instructions were followed in all subsequent steps. cDNA libraries from different samples were multiplexed using Illumina RPI oligonucleotides and sequenced on a HiSeq 2000 instrument using 1x101+7 cycles. Illumina Truseq RNA v2 kit (unstranded)