GSM4474586: AMs from PBS-treated control mouse 1; Mus musculus; RNA-Seq
AMs were flow cytometry sorted and immediately lysed in QIAGEN RLT buffer supplemented with 1 % 2-mercaptoethanol. Lysates were passed through QIAShredder columns by centrifugation and RNA was extracted from lysates using the RNeasy Plus Micro Kit, as per manufacturer's instructions. 200 ng RNA per sample was polyA-purified and converted to cDNA sequencing libraries using the Illumina TruSeq Stranded mRNA v2 kit, with unique indexes per sample to allow multiplexing.