GSM3668260: LMNA; Mus musculus; OTHER
MEFs were collected by trypsinization and DNA was isolated using QIAamp DNA Mini kit (Qiagen,  51304), followed by ethanol precipitation and resuspension to 1 µg/ul in 10 mM Tris, pH 8.0.  DamID was performed as described previously (L.Guelen et al, Nature 2008) post-DamID amplified material was randomized  by performing an end repair reaction, followed by ligation and sonication.  Randomized fragments were subjected to sequencing library preparation with Illumina Paired End Sequencing Kit.