Small RNA sequencing of Anemonia viridis from pH 8.2
Total RNA from three different tissue samples of an individual (body wall, tentacles and oral disc) was pooled at equal amounts. The small RNA fraction was enriched using PureLink miRNA Isolation Kit (ThermoFisher Scientific, Waltham, MA, USA). Libraries were prepared only from high quality RNA samples (RIN ? 7) following the SOLiD Total RNA-Seq Kit protocol (ThermoFisher Scientific, Waltham, MA, USA).