GSM4040615: CPhet10; Mus musculus; RNA-Seq
RNA was extracted from the frozen prostate tumours or tissues of WT (n=3; 12 months age), Ptenpc-/- (n=3; ~14 months), Ctnnb1pc(ex3)Δ/+ (n=3; ~12 months), Ctnnb1pc(ex3)Δ/+ Ptenpc+/- (n=3; ~6-8 months) and Mock (n=2; ~15 months) and ADT (n=3; ~15 months) treated Ctnnb1Nkx3-1CreERT2(ex3)Δ/+ PtenNkx3-1CreERT2(ex3)Δ+/-. RNA quality of RNA extracted was evaluated by RNA integrity number (RIN>7.3), calculated using an Agilent 2100 Bioanalyzer (Agilent Technologies) with the Agilent RNA 6000 Nano Kit.  The library preparation and sequencing was carried out by BGI (Beijing) using the following steps and reagents according to the manufacturer's instructions. The library was pair end sequenced on the Illumina® HiSeq 2000 platform, with 90 nucleotide reads and at least 6 Gb of reads generated per sample.