Drosophila mauritiana  Paired-End Genome Sequencing
Genomic DNA was isolated using the Qiagen DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany) according to the manufacturer´s instructions, and 5 ug were used for construction of paired-end libraries. After shearing the DNA using the Covaris S2 system (Covaris, Inc. Woburn, MA).    Illumina paired-end libraries were generated following the instructions of the Illumina Paired-End Sample Preparation Kit. Two libraries with different fragment sizes (220 bp and 590 bp) were generated for paired-end sequencing.