GSM4039339: 7h-VCP5; Homo sapiens; RNA-Seq
Total RNA was extracted from exposed and control HepaRG™ cell pellets (n=3) using the Qiagen RNeasy Mini kit (Qiagen, Toronto, Ontario, Canada) with an on-column DNase I digestion, according to the supplier's protocol. Purified RNA was quantified and assessed for quality with a NanoDrop® ND-1000 spectrophotometer and an Agilent TapeStation 2200. High quality RNA was used for gene expression analysis (A260/280 ≥ 2.0 and RINe ranging from 8.3 to 10). The human AmpliSeq library kit was used to build the libraries. RNA-Seq (Human Whole Transcriptome AmpliSeq)