Phytoalexins responsive genes support Bursaphelenchus xylophilus survival from the stress of Pinus massoniana
Total RNA was extracted from the nematode powder using TRIzol and then cleaned using an RNeasy Minikit column. The OD260 nm/OD280 nm and OD260 nm/OD230 nm values of the extracted RNA were quantied using a NanoDrop. Then, 4 cDNA libraries representing P. massoniana inoculation, CKB. cinereal, Carvone treated, and CKCarvone were prepared and sequenced by BGI-Shenzhen according to the BGISEQ-500 standard protocol.