GSM3591407: monocyte_donor1; Homo sapiens; RNA-Seq
SeqWell analysis was performed as described in Gierahn et al. (Nature Methods, 2017) Briefly, at the respective timepoint, cells were detached using trypsin, spun down, and counted. Approximately 12,000 cells were loaded on each array for each timepoint and condition to minimize doublet-loading. The arrays were sealed with a semi-permeable membrane prior to cell lysis and hybridization to single-cell beads. Beads were subsequently pooled for reverse transcription and whole transcriptome amplification. RNA libraries were prepared for sequencing using standard Illumina protocols