RNA-Seq of  Pochonia chlamydosporia mycelium, grown inCzapek Dox broth (Oxoid, Basingstoke, UK)  for 7 days at 26C in dark with agitation
The tissues were powdered in liquid nitrogen. Total RNA was extracted and purified by using RNeasy Plant Mini Kit (Qiagen, Milan, Italy), following the manufacturer's protocol. cDNA libraries  were prepared from 4 g total RNA using TruSeq RNA Sample Preparation Kit v2 (Illumina, Inc., San Diego, California, USA) and validated according to Illuminas low-throughput protocol.