GSM3211391: Hi-C; Homo sapiens; Hi-C
Five million cells were crosslinked with 1% formaldehyde for 10 min at room temperature, then digested with 125 units of MboI, and labeled by biotinylated nucleotides and proximity-ligated. After reverse crosslinking, ligated DNA was purified and sheared to 300-500 bp, then ligation junctions were pulled down with streptavidin beads and prepared as general Illumina library. The Hi-C sample underwent 76-cycles paired-end sequencing on the Illumina HiSeq 4000.