miRNA-Seq of  Zea mays: leaves
For the library construction, total RNA was first extracted from nine samples. The small RNA was ligated with 3 adaptor, 5 adaptor, transcribed to cDNA and followed by PCR amplification. The PCR products were run on PAGE (polyacrylamide gel) and purified. The purified PCR were sequenced on Illumina Hiseq platform.