UCSD-002.1
The NCI-60 cell line panel was grown in RPMI-1640 with 10% FBS under standard cultureconditions. The PDX cell lines were cultured in DMEM/F-12 media supplemented withGlutamax, B27, EGF, FGF, and Heparin. Lymphoblastoid cells were grown in RPMI-1640,supplemented with 2mM glutamine and 15% FBS. IMR90 and ALS6-Kin4 cells were grownin DMEM/F-12 supplemented with 20% FBS. DNA library preparation DNA was sonicatedto produce 300-500 bp fragments. DNA end repair was performed using End-it (Epicentre),DNA library adapters (Illumina) were ligated, and the DNA libraries were amplified. Paired-end next generation sequencing was performed and samples were run on the Illumina Hi-Seq 1000. Sequenced reads were mapped to UCSC human assembly version hg19 using bwa mem version 0.7.9a