GSM2453482: CsiR_ChIPSeq; Escherichia coli K-12; ChIP-Seq
For chromatin immunoprecipitation, cells were fixed with formaldehyde and glycine and sheared via sonication. Sheared DNA from lysate was immunoprecipitated (IP) out using an anti-FLAG mAb (Sigma). DNA was washed with IPP150 buffer and purified via ProteinaseK digestion and Qiaquick PCR purification kit (Qiagen - Cat. No. 28106). Total RNA extraction was done using TRIzol reagent and DNase digested. RNA purfication was carried out using Rneasy spin columns The appropriate protocols were performed using standard Illumina procedures