GSM2303101: RPF_△yNaa10; Saccharomyces cerevisiae; OTHER
Cryogenic grinding was performed with 1.4 g dried yeast in a SPEX Freezer mill for 15 cycles at 3 min 15 cps, followed by 3 min cooling. Obtained cell powder was resuspended in 2.5 ml polysome buffer (ARTseq Ribo Profile kit, Illumina). ARTseq Ribo Profile kit, Illumina