mouse adult cardiac myocyte nuclei rep1: noncoding RNAseq
Total RNA was isolated from FACS sorted cardiomyocyte nuclei from one adult mouse heart using RNeasy Micro Kit (Qiagen) including on-column DNAse digestion. Ribosomal RNA was depleted using the Low Input RiboMinus Eukaryote System v2 (Life technologies). Depleted RNA fragmented to approximately 200 bp fragments and was used for library construction (NEBNext® Ultra RNA Library Prep Kit for Illumina, NEB). Size selection was performed with AMPure® XP Beads (Beckman Coulter) according to the NEBNext® Ultra RNA Library Prep Kit manual. 15 PCR cycles were used for amplification.