Degradome-seq
In brief, poly-A-containing mRNA was purified from total RNA, and then a 5RNA adaptor containing aMmeI recognition site was ligated to the 5-phosphate of the poly-A RNA. Subsequently, reverse transcription was performed to generate first-strand cDNA, followed by digestion with MmeI and ligation to a 3adaptor. Finally, the ligation products were amplified using PCR, gel-purified and subjected to deep sequencing on the Illumina HiSeq 2000