GSM2263707: Col-0 biological replicate C 25 °C; Arabidopsis thaliana; RNA-Seq
For each sample, tissue from ~10 plants was collected. Using jeweler's forceps, leaves were removed to obtain SAM enriched tissue. Total RNA was extract using the InviTrap Spin Plant RNA Mini Kit ( REF: 1064100300) from Invitek according to the manufacturer's protocol. DNAse treatment was performed to remove genomic DNA. DNase I digestion was performed on total RNA using Turbo DNase I from Ambion. RNA integrity was checked on 1% (w/v) agarose gel after DNase I treatment. Samples were prepared for sequencing using the TruSeq Stranded mRNA Sample Prep kit (REF 15032613) from Illumina according to the manufacturer's protocol.