GSM2304371: cells after 2hr recovery replicate 1; Homo sapiens; RNA-Seq
Total RNA was extracted using mirVana miRNA isolation kit (Thermo Fisher Scientific) then treated with TURBO Dnase (Thermo Fisher Scientific) to get rid of the genomic DNA. Ribosomal RNA (rRNA) was removed using RiboMinus Eukaryote System v2 (Thermo Fisher Scientific). cDNA libraries used for sequencing were made from rRNA-depleted RNA using Ion Total RNA-seq kit v2 (Thermo Fisher Scientific)