GSM3067335: C4-2B MA4-022-1; Homo sapiens; RNA-Seq
Total RNA was prepared from C4-2B using the Qiagen RNeasy kit protocol followed by DNAseI treatment.  1-2ug of total RNA was used for the construction of the libraries. The Illumina Hi-seq platform with 50 bp single end sequencing. Total RNA-Seq libraries were constructed from 1-3 μg total RNA. Total RNA was treated with the Ribo-Zero™ Magnetic Gold Kit (Human/Mouse/Rat) from Illumina (cat. #MRZG126), and libraries were prepared from rRNA depleted samples using ScriptSeq™ v2 RNA-Seq Library Preparation Kit from Illumina (Cat. #SSV21106). Briefly, rRNA depleted samples were fragmented, reverse transcribed, fitted with appropriate adapters and amplified. RNA-Seq libraries were sequenced on an Illumina HiSeq to a sequencing depth of 40-50 million reads (50 bp single read).