GSM3066269: NCP3; Acipenser sinensis; RNA-Seq
Palates were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Total RNA of each sample was quantified and qualified by Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA), NanoDrop (Thermo Fisher Scientific Inc.) and 1% agrose gel. RNA libraries were prepared for sequencing using standard Illumina protocols