GSM4156579: HEPG2-2; Homo sapiens; RNA-Seq
Total RNA was isolated with TRIzolTM reagent (Invitrogen, USA)/RNeasy Mini Kit (Qiagen) according to the manufacturer's instruction. RNA concentration was measured using Qubit® RNA Assay Kit in Qubit® 2.0 Fluorometer (Life Technologies, CA, USA). RNA purity was assessed using the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA). RNA integrity was checked using the RNA Nano 6000 Assay Kit on the Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA), NanoDrop (Thermo Fisher Scientific Inc.) and 1 % agrose gel. Only samples (1μg total RNA) with an RNA integrity number (RIN) of ≥ 7 were subjected to deep sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols(NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®).