GSM4155836: Nanopore_4h; Saccharomyces cerevisiae; RNA-Seq
Cells were collected by filtration and then flash frozen in liquid nitrogen. RNA was extracted by AcidPhenol:Chloroform:Isoamyl alcohol isolation. PolyA selection was performed with 100 ug of RNA and oligo-dT DynaBeads. Using 500 ng of polyA RNA, libraries were prepared with the Direct RNA Sequencing Kit (SQK-RNA001) from Oxford Nanopore Technologies.