GSM2977653: Snord116+/- ZT9_rep1; Mus musculus; Bisulfite-Seq
Mice were sacrificed at ZT0, 3, 6, 9, 12, and 16.  Genomic DNA was isolated from 5-10mg cortex using the Gentra Puregene kit (Qiagen) 500ng DNA was bisulfite converted using the EZ DNA Methylation-Lightening kit (Zymo).  Libraries were prepared with 100ng bisulfite converted DNA using the TruSeq DNA Methylation kit (Illumina) and barcoded using TruSeq DNA Methylation Kit Index PCR Primers (Illumina).