GSM4474626: PAO1_1; Pseudomonas aeruginosa; RNA-Seq
Cells were lysed by bead beating and RNA was precipitated using chloroform and isopropanol. Pellets were washed with ethanol and quantified using NanoDrop. mRNA was enriched using Ambion MICROBExpress kit. Enriched mRNA was fragmented using NEBNext Fragmentation Module kit and libraries were prepared using NEBNext Multiplex Small RNA Library Prep kit.