GSM2453476: CE1_Del_clone2_TSS1; Homo sapiens; OTHER
3C DNAs were digested with a second restriction enzyme, DpnII, religated, and purified using Qiagen PCR purification columns. The circularized DNA was amplified using inverse PCR and nested inverse PCR reactions with primers in the anchor HindIII-DpnII fragment. PCR products were used to prepare indexed sequencing libraries. All twelve libraries were pooled in one lane for 50 bp single-end deep sequencing (Illumina HiSeq2500). 4C libraries were prepared for sequencing using standard Illumina protocols