GSM4475191: Mecr, Day 1, replicate A; Mus musculus; RNA-Seq
Total RNA was extracted from one confluent 10 cm dish per replicate using TRI Reagent (Zymo Research, R2050-1-200) according to the manufacturer's protocol. Poly(A) RNA was purified from total RNA samples (100-500 ng) with oligo(dT) magnetic beads. Stranded mRNA sequencing libraries were prepared as described using the Illumina TruSeq Stranded mRNA Library Prep kit (20020595) and TruSeq RNA UD Indexes (20022371). Librarieswere sequenced on an Illumina NovaSeq instrument using 2 x 51 cycle paired end sequencing.