GSM2365991: classic dentritic cell_DC-F4; Homo sapiens; RNA-Seq
For the single cell, Fluidigm C1 system was used. For bulk cell, 2,000-30,000 progenitor cells of each population were purified using flowcytometry sorting. After cell lysis, mRNA was purified using LNA-Oligo-(dT) coated streptavidin-coated C1 DynabeadsRNA was extracted RNA was amplified with clonetech SMARTer Ultra Low RNA Kit (634935 and 639207, Clontech)