WGS sequencing of Sphenodon punctatus
High-molecular-weight genomic DNA was extracted using the Qiagen Genomic Tip DNA extraction kit (genomic-tip column 100/G) from 100ul of snap-frozen blood according to the manufacturer's directions, and performed at the Laboratories of Analytical Biology at the Smithsonian Institutions National Museum of Natural History (NMNH). A PacBio SMRTbell library was prepared using the SMRTBell Express Template Preparation Kit (Pacific Biosciences) following the PacBio 15-kb template preparation protocol, with a minimum size cutoff of 5kb size selection used in the size selection stage with the Sage Science BluePippin system. The prepared library was run on the PacBio Sequel platform (Pacific Biosciences) using version 2.1 chemistry. The Single Molecule Real-Time (SMRT) Cell was sequenced on a SMRT cell with 360min movie lengths.