GSM2804778: cDC1_B6g7_PBS_2; Mus musculus; RNA-Seq
Total RNA was extracted using Arcturus PicoPure RNA isolation kit per the manufacturer’s protocol Low-input RNA sequencing libraries were generated from about 300 pg of total RNA using SMART-seq v4 Ultra Low Input RNA kit (Clontech, CA). All samples were subjected to 14 PCR amplification cycles to minimize PCR biases (Bhargava et al., 2014). Amplified cDNA libraries were later fragmented through sonication to obtain 200 – 500 bp fragments. Standard Illumina sequencing libraries were prepared using Rubicon ThruPLEX DNA-seq kit (Rubicon Genomics, MI) according to manufacturer’s protocol. Sample barcoded libraries were sequenced on Illumina’s NextSeq 500 instrumentation using NextSeq 300 Cycle Kit, High Output, V1 reagents (Illumina, CA) and data analysis workflow, bcl2fastq -v2.17.1.14, to obtain 150 bp paired-end reads.