GSM3669473: 17+ Bound (6); Homo sapiens; ChIP-Seq
TGs were homogenized, crosslinked in 1% formaldehyde, and the cell lysates were sonicated.  The sheared chromatin was pre-cleared with salmon-sperm DNA-protein A-agarose beads prior to antibody incubation. An aliquot representing 1/5 of the total sample volume was removed as a sample input (I).  Both bound and input fractions were treated with 5M NaCl, RNAse A and proteinase K and the DNA was finally purified using a Qiaquick PCR purification kit. Prior to Illumina library construction and hybridization capture-based target enrichment. Briefly, 8 PCR-cycle indexed libraries were pooled for selection using the SureSelect XT hs system with custom probes spanning the HSV-1 genome (Roche). Samples were subject to 4 additional PCR cycles and used for 100  base pair paired-end Illumina sequencing on the HiSeq platform. Kappa biosystems hyperprep