GSM2720075: tg-5; Mus musculus; RNA-Seq
Trigeminal neurons were isolated using the postnatal neural tissue dissociation kit (Miltenyi Biotec), followed by a 12.5%/20% percoll gradient.  Glia and other non-neuronal cells were then depleted using the mouse neuron isolation kit (Milteny Biotec). Libraries construction were performed as described by the Drop-seq protocol from the McCarroll lab