degradome-seq of Camellia sinensis: leaves infected by Didymella segeticola isolate GZSQ-4
Samples from 15 plants at each treatment group were pooled together to avoid variations in individual plants due to pathogen inoculation. Leaf samples were ground into fine powder under the protection of liquid nitrogen. About 100 mg powder of each tea leaf was used for RNA extraction. After total RNA was isolated and purified using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer's procedure. Poly(A) RNA is purified from plant total RNA(20ug) using poly-T oligo-attached magnetic beads using two rounds of purification. Then the cDNA were amplified with PCR. The average insert size for the final cDNA library was 200-400 bp. At last, we performed the 50bp single-end sequencing on an Illumina Hiseq2500 (LCBio,China) following the vendor's recommended protocol.