GSM3209214: GRH162; Drosophila melanogaster; ncRNA-Seq
Ovaries were hand dissected, flash frozen on liquid nitrogen, and RNA was harvested using Trizol reagent. A small RNA fraction of 18nt to 30nt in length was obtained by separating it on a denaturing polyacrylamide gel from total RNA extracted from dissected ovaries. This fraction was used to generate multiplexed libraries with Illumina TruSeq Small RNA library preparation kits (RS-200-0012, RS200-0024, RS-200-036 or RS-200-048) at Fasteris (http://www.fasteris.com).  A house protocol based on TruSeq, which reduces 2S RNA (30nt) contamination in the final library, was performed. Libraries were sequenced using Illumina HiSeq 2500.