GSM2803962: rpoB-Phosphate-depleted_rep1; Mycobacterium tuberculosis; RNA-Seq
Bacteria were spun down at 4C and immersed in Trizol. Mixture was then beat-beated for 4 minutes. Chloroform:Isoamyl (24:1) was used to extract the RNA. Turbo DNase was used to remove remaining gDNA. RNA was converted to cDNA and an NGS library was constructed using the Encore Complete Prokaryotic RNA-Seq DR Mulitplex Systems 1-8 and 9-16 kit from NuGen (Part No. 0326 and 0327).