GSM2304556: Wild-type-VitD treated 4; Caenorhabditis elegans; RNA-Seq
Pools of individual worms (40 per pool) were a single biological replicate, and were extracted via a quiagen nucleic acid extraction robot using an Invitrogen RNAeasy mini kit as per the manufacturers instructions. Libraries were constructed and run on a HiSeq 2000 instrument at the University of Minnesota sequencing Core as per their standard SOPs from Illumina. Libraries were size selected to achieve an average insert of 200bp, and a 50 base pair paired end protocol was used. Greater than 20 million reads of coverage were obtained for each sample.