A.fumigatus with A549 cells
RNA sequencing was performed at ServiceXS (Leiden, The Netherlands) using illumina NextSeq 500 according to manufacturer protocols. The NEBNext ultra Directional RNA library Prep kit for Illumina was used for cDNA synthesis. Briefly, the poly-A containing mRNA was isolated from 10  g total RNA using poly-dT oligo magnetic beads. After fragmentation of the mRNA, a cDNA synthesis was performed. Adapters were ligated to the cDNA and amplified by PCR to create the final cDNA library. Quality and yield was measured with the Fragment Analyzer. Size of the cDNA fragments was between 300 and 500, as expected. A concentration of 1.6 pM of DNA was used for sequencing. RNA-seq was performed on RNA isolated form two independent experiments for each condition.