GSM3901927: Hdac3-Emx1-KO1-RNA; Mus musculus; RNA-Seq
E16.5 cultured neurospheres or P0 cortices were collected into clean eppendorf tubes containing 0.7 ml QIAZOL (Qiagen) and made into single cells by passing though needles. Total RNA was isolated using miRNeasy Mini kit (Qiagen). The concentration and integrity were assessed by the sequencing facility (Omega Bioservices) using a RNA screentape. Once passed the sample quality control, the samples were used for oligo-dT primed RNA-Seq (Illumina TruSeq stranded mRNA) on HiSeq2500 sequencers (Illumina) RNA library were prepared for sequencing using standard Illumina protocols