GSM2455276: Embryoid body with ATRA 0day; Mus musculus; RNA-Seq
Total RNA was prepared from cultured EBs using TRI Reagent (Molecular Research Center, Inc., Cincinnati, OH) and cDNAs were synthesized with 3 ug of total RNA using a RT system kit with oligo deoxythymidine (dT) 18 primers (Bioneer, Daejeon, Korea). RNA libraries were prepared for sequencing using standard Illumina protocols