GSM3131992: 4260-2-S; Homo sapiens; RNA-Seq
At termination of cultures, activated and resting cells were recovered and centrifuged at 500 x g for 5 minutes in RPMI media and cell pellets lysed in 350µL of RLT buffer from the Qiagen AllPrep DNA/RNA extraction micro kit (Qiagen). Genomic DNA and total RNA were extracted according to manufacturer's instructions using the QIACube automated liquid handling robot (Qiagen). Library preparation was performed using the Illumina TruSeq stranded mRNA kit with a starting input of 50ng and libraries were sequenced on the Illumina HiSeq 4000 instrument generating an average of 20 million reads per sample.