GSM4654158: 6_2; Homo sapiens; RNA-Seq
Total RNA isolation was performed with Tempus™ Spin RNA Isolation Kit (Thermo Fisher Scientific), according to the manufacturer's instructions. Ribosomal RNA depletion was done with Low Input RiboMinus™ Eukaryote System v2 (Thermo Fisher Scientific, USA) by processing 500ng of total RNA from each sample. For cDNA library preparation we used Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific, USA), sequencing was done on Ion Proton™ System and Ion PI™ Chip (Thermo Fisher Scientific, USA), following the manufacturer's instructions.