GSM3209242: siMM replicate 1 IL-1; Homo sapiens; RNA-Seq
After stimulation cells were directly lyzed in TRIZol and total RNAs were extracted according to the manufacturer's protocol Total RNA libraries were generated using the NEBNext Ultra II Directional RNA Library Kit (New England Biolabs #E7765) after rRNA-depletion using the NEBNext rRNA depletion kit (New England Biolabs #6310). To facilitate multiplexing oif libraries indexed primers (New England Biolabs #E7335) were used. 500 ng of total RNA per sample were used as input material.