GSM4259327: 2a-HET2_AMY; Mus musculus; RNA-Seq
For RNA-Seq, amygdala or hippocampal issue was homogenized in Tri Reagent (Sigma). Samples were subjected to total RNA isolation, and RNA was purified using RNEasy Mini Kit (Qiagen). ERCC spike-in RNA was added at this stage, according to manufacturer's instructions (Life Technologies). For RNA-Seq, libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit with oligo-dT priming. Multiplexed libraries were pooled in approximately equimolar ratios and purified using Agencourt RNAClean XP beads (Beckman Coulter).