GSM3901661: Kidney collecting duct cells, Sham rep2; Mus musculus; RNA-Seq
Total RNA was eluted and purified using RNeasy Micro Kit (Qiagen) according to the manufacturer's instructions. RNA integrity number (RIN) was measured using the Agilent 2200 TapeStation (Agilent Technologies, TX) and only samples with a RIN than or equal to 6.8 was used. Approximately 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols