GSM4039570: DMSO_1_GRO-seq; Homo sapiens; OTHER
Nuclei were isolated from pre-treted HeLa cells using 27G needle in lysis buffer. Nuclear Run-on was proceed for 5 min in the presence of Sarkosyl and Br-UTP. RNA was extracted and fragmented and then BrU-labeled RNA was purified with anti-BrdU beads. GRO-seq RNA samples were reverse transcribed and amplified (15 cycles) using inhouse mRNA Seq protocol w/o PolyA capture with random hexamers.