Compete closed genomes of ten Shiga toxin-containing Escherichia coli O157:H7 strains             from New Zealand
Single Molecule, Real-Time (SMRT) sequencing was performed on the                 Pacific Biosciences (PacBio) RSII sequencing platform using PacBio SMRTbell Template                 Prep kit 1.0., with the P6/C4 sequencing chemistry and 360-min data collection                 protocols. DNA was extracted using 100/G columns (Qiagen), sheared using a Corvaris                 g-tube, and libraries were prepared from 10 mg of bacterial genomic DNA, using the                 standard protocol, and processed for sequencing as recommended by the                 supplier.