GSM4040583: HN2-INPUT; Mus musculus; ChIP-Seq
Minced frozen prostate tissue was fixed using 1% formaldehyde for 15 mins and quenched with Glycine (125mM) at room temperature for 5 mins.  Tissues were homogenised, nuclei isolated and lysed.  Nuclear lysates were sonicated using the Bioruptor sonicator on high setting 30s on/30s off for 25 minutes (2x 10 minutes + 1x 5 minutes). Pre-cleared chromatin samples using IgG were used for ChIP assay with AR antibody with protein G-agarose suspension. 10% input control was stored separated at -20°C. After ChIP at 4°C with rotation overnight, the pellet was washed and DNA isolated and dissolved for sequencing which was carried out at BGI Libraries were prepared according to Illumina's instructions