GSM2977378: wt foxp3＋Treg cell; Mus musculus; RNA-Seq
Splenic CD4+Foxp3+ (YFP+) cells were sorted from 2-week-old poh1fl/fl Foxp3Cre+ or poh1+/+ Foxp3Cre+ mice, using MoFlo XDP. RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit v2 (Cat#RS-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols