GSM2454699: WT_1hr_rep3; Arabidopsis thaliana; ssRNA-seq
Total RNA was extracted from Arabidopsis 2-week old seedlings using TRIzol reagent (Ambion), treated with TURBO DNase (Ambion) and purified using RNeasy mini spin column (Qiagen). The quality of purified RNA was assessed using an Agilent 2100 Bioanalyzer. cDNA libraries for ssRNA-seq were prepared using the Illumina TruSeq Stranded mRNA Sample Preparation Kit according to the low sample protocol guidelines. The quality and size of each sample library was assessed using an Agilent High Sensitivity D1K ScreenTape System. The average sizes of the enriched cDNA fragments were between 272 and 300 bp. The three biological replicates for each condition (negative control, 1 h_elf18, 6 h_elf18, and 12 h_elf18) were pooled into one well, and sequenced on an Illumina NextSeq High Output SR 75 with 75-cycle single reads per multiplexed sample.