GSM2720353: ATF6+/+ hMSC tunicamycin-1 IgG ChIP-seq; Homo sapiens; ChIP-Seq
Total RNA was extracted  with Trizol reagent. To obtain the genomic DNA  fragments of interest, the cells were crosslinked and lysed, and then the chromatin was sheared into fragments using sonication. Then samples were incubated with anti-H3K4me3 antibody(Abcam, ab8580) or anti-FLAG(Sigma, F1804) antibody overnight. The DNA was de-crosslinked and extracted on the next day. 1.5 μg of total RNA was used to construct RNA sequencing libraries by TruSeq RNA Sample Preparation Kit (Illumina) following the manufacturer’s recommended protocol. After the DNA was de-crosslinked and extracted, the ChIP-sequencing library was constructed by TruSeq DNA Sample Preparation Kit (Illumina) or NEBNext® DNA Library Prep Reagent Set (NEB) according to the manufacturer’s instructions.