GSM2720171: WT 3; Mus musculus; RNA-Seq
CD11c+ cells from BM culture were isolated by magnetic sorting with biotin-CD11c (clone N418) and MojosortTM  streptavidin nanobeads (BioLegend; purity>90%). Total RNA was extracted using RNeasy Mini Kit (QIAGEN, Germantown, MD) and submitted to Purdue University Genomic Core Facility for sequencing. The samples were processed with the Illumina TruSeq Standed mRNA Sample Prep Kit (Illumina Inc. San Diego, CA). Following quantification with KAPA Library Quantification Kit (KAPA Biosystems, Wilmington, MA). The libraries were sequenced with a HiSeq 2500 using v3 chemistry to generate paired-end 100 base reads.