Hi-C of Vitis vinifera (Pinot Noir) young leaf tissue, MboI
Nuclei isolated from fixed leaf tissue were incubated with MboI restriction enzyme (New England Biolabs, R0147M) and the ends were filled in with a biotinylated nucleotide before ligation of chromatin with T4 DNA ligase. DNA was sonicated, end-repaired, A-tailed, and Illumina TruSeq adapters were ligated prior to PCR amplification with Illumina primers.