mlh3-D523N_8b
Spore colonies from the four viable spore tetrads of SK1-mlh3-D523N in S288c/YJM789 hybrid were grown overnight at 30°C in four ml YPD broth. DNA was isolated from the culture using PrepEase DNA isolation kit (Affymetrix). Whole genome sequencing on Illumina platform was performed at Fasteris,Switzerland.