GSM4041131: PC8_NoIndex_L006; Pneumocystis murina; RNA-Seq
RNA was extracted from separated troph and asci life froms using the Illumina TruSeq Stranded mRNA sample preparation kit The cleaved RNA fragments were then copied into first strand cDNA using reverse transcriptase and random primers. Strand specificity was achieved by using dUTP in the Second Strand Marking Mix, followed by second strand cDNA synthesis using DNA Polymerase I and RNase H cDNA libraries were validated using KAPA Biosystems primer premix kit with Illumina-compatible DNA primers and Qubit 2.0 fluorometer