16s rRNA seq of Trichuris muris-infected mice in the lab versus field: feces
16S gut microbiota analysis was performed on DNA extracted from frozen murine stool samples. Amplicon libraries of the bacterial 16S-V4 hypervariable region was prepared using dual-indexed Illumina primers (515F/806R) as described by Kozich et al. (2013). Sequencing was performed in a 2x250bp paired end format using a 500 cycle v2 reagent cartridge at Michigan State University.