Illumina sequencing of Human HepG2 cell line transcript fragment library
Total RNA was extracted from cultured hepatocellular carcinomal cell line Hep G2 (ATCC HB-8065), and analysed using a 5'RACE kit (Ambion) with 17 different gene-specific nested primer pairs. The inner PCR primers contained tails matching the proprietary sequence of the Illumina Genome Analyzer platform, that was used to sequence the RACE PCR products after they were pooled together and purified with a Qiagen kit. Reads were then aligned to the human genome using the Nexalign program (Lassmann et al, in preparation).