GSM4405468: VEH2; Homo sapiens; RNA-Seq
Cells were sorted into 5µl Vapor-Lock (Qiagen) containing a droplet with primers and dNTPs. Cells were lysed at 65 degrees Celsius for 5 minutes. mRNA was then reverse transcribed by adding RT mix. RNA was processed by an automated version of the previously described CEL-Seq2 technique. Libraries were sequenced on an Illumina NextSeq500 using 75bp paired end sequencing.