Adzuki bean borer (ABB) L4 larvae on maize - replicate1 (Sample10)
Total RNA was extracted from each L4 (Quiagen AllPrep DNA/RNA 96kit). RNA was quantified (Nanodrop) and equimolar amounts of individual extracts were pooled to obtain 12 samples corresponding to the three replicates of the four experimental set-ups. Tagged cDNA libraries were constructed with the TruSeq Stranded mRNA Sample Preparation Kit (Illumina) at MGX (Montpellier, France). Paired-end 100 bp sequencing was performed on three lanes of an Illumina HiSeq 2000.