GSM4474274: S3_RES_W0; Homo sapiens; miRNA-Seq
Exosomes were extracted from 2mL of plasma using size exclusion chromatography, and further enriched for those of neuronal origin using an immunoassay. Small non-coding RNA was isolated using miRNeasy kit Librarys were constructed using the 4N library prep protocol from the Galas lab. (Giraldez et al., 2018) First, degenerative adapters are added to the miRNA. These adapters are used to eliminate the problem of bias in small RNA library construction. The product is then reverse transcribed and amplified.