GSM3417011: ASO_CaptureSeq_rep3_antisense; Homo sapiens; OTHER
Chromatin was purified and chromatin-associated nucleic acids were isolated. Following the hybridization of the biotinylated ASO to NEAT1, NEAT1 and associated DNA complexes were captured on streptavidin beads. NEAT1-associated DNA was eluted and sequenced. DNA libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) and NEBNext Multiplex Oligos for Illumina (NEB).