GSM3208869: Oncopeltus_fasciatus_buffer_RNA-seq_Ovary_individual2; Oncopeltus fasciatus; RNA-Seq
Gene expression. RNA-seq libraries for RNA extracted from three DNMT1 knocked down and three control individuals were constructed using Illumina TruSeq Stranded RNA LT Kit (Illumina, San Diego, CA) following the manufacturer's instructions with limited modifications. The starting quantity of total RNA was adjusted to 1.3 µg, and all volumes were reduced to a third of the described quantity. Tissue was flash frozen in liquid nitrogen and stored at -80°C until processing. Total RNA and genomic DNA was extracted using a Qiagen RNAeasy micro kit (Qiagen, Venlo, The Netherlands) per manufacturer's instructions with Qiagen Qiazol as the lysis buffer. Complementary DNA (cDNA) was synthesized from 500 ng RNA with qScript cDNA SuperMix (Quanta Biosciences, Gaithersburg, MD).