GSM3728943: ATAC-seq D2; Sparassis latifolia; ATAC-seq
ATAC-seq: Harvest and count cells. Cells should be intact and in a homogenous, single-cell suspension. Centrifuge 50,000 cells 5 min at 500 × g, 4°C. The number of cells at this step is crucial, as the transposase-to-cell ratio determines the distribution of DNA fragments generated. See Critical Parameters. Remove and discard supernatant. Wash cells once with 50 μl of cold PBS buffer. Centrifuge 5 min at 500 × g, 4°C. Remove and discard supernatant. Gently pipet up and down to resuspend the cell pellet in 50 μl of cold lysis buffer. Centrifuge immediately for 10 min at 500 × g, 4°C. Discard the supernatant, and immediately continue to transposition reaction. follow the protocol: ATAC-seq: A Method for Assaying Chromatin Accessibility Genome-Wide