GSM2453309: mst2D RNA-seq; Schizosaccharomyces pombe; RNA-Seq
Total RNA was isolated from exponentially growing cells using hot phenol. For sRNA-Seq, the RNA was fractionated using RNeasy Midi columns (Qiagen). The flow-through fraction was precipitated and aliquots of 25 ug were separated by 17.5% PAGE to isolate the 18–28-nucleotide fraction. Libraries were prepared using either the TruSeq Stranded mRNA library Prep Kit (for RNA) or the Illumina TruSeq small RNA kit according to manufacturers instructions. For barcodes, see sample characteristics.