GSM2719779: Invader 4 LTR ctrl Recovery 3 days; Drosophila melanogaster; Bisulfite-Seq
Bisulfite conversion on 5 µg of genomic DNA was carried using the EpiTect Bisulfite Kit (Qiagen) according to the manufacturer’s instructions. 200 ng bisulfite-treated DNA was used as template in a first PCR reaction (35 cycles, annealing temperature= 50°C) to amplify regions in the canonical Invader4 LTR. Amplicons were gel-purified and 50 ng were used as template for a second PCR reaction (15 cycles, annealing temperature= 50°C) to re-amplify the amplicon and adding 454-sequencing adapters.