GSM3132053: MZ-CRC-1 12h_20nM_1; Homo sapiens; RNA-Seq
At the desired exposure time, the supernatant and scraped cells were collected into 50mL tubes, centrifuged at 300xg for 5min, the supernatant aspirated, and the pellet washed with 1mL of PBS and transferred into 1.5mL tubes. After a centrifugation step at 500xg for 5min, the supernatant was aspirated and the pellet resuspended in 1mL of TRIzol reagent (Invitrogen) to isolate the total RNA present in the cells. The RNA isolation was performed following the protocol provided by Invitrogen for TRIzol reagent use. TruSeq Stranded Total RNA Ribo-Zero H/M/R Gold (RS-122-2301)