GSM4476130: tdtomato_P2; Mus musculus; RNA-Seq
Brains and spinal cords were harvested at day 30 p.i. and processed as described above. After Live/Dead staining and treatment with Fc block, OLs were identified using APC-anti-O4 (1:50; Miltenyi). RNA was extracted from sorted OLs using an RNeasy Micro Kit (Qiagen). Total RNA samples were converted to sequencing libraries using Takara Bio's SMARTer Stranded Total RNA-Seq – Pico Mammalian Kit v2 (Cat. #634414).