GSM2544762: ScY01; Saccharomyces cerevisiae; RNA-Seq
Cells of ScY and ScY01 were grown on YP medium containing 200 g/l glucose at 40ºC at 200 rpm for 14 h ~ 16 h to the early-log phase, and harvested in Falcon tubes precooled in liquid N2 by centrifuging for 5 minutes at 4000 rpm (3220 × g). The supernatant was discarded, and the pellet was stored at −80°C until further use. Total RNA extraction, library construction and sequencing were conducted by Majorbio Company (Shanghai, China). RNA libraries were prepared for sequencing using standard Illumina protocols