16S rRNA amplicon Sequencing of the skin microbiome : adult female forearm
16S rRNA (V1-V3) profiles were generated using Illumina MiSeq. gDNA was isolated from skin swabs with the NukEx Pure RNA/DNA Kit. A 2-step PCR strategy was chosen with primers 8F-YM and 517R for the initial PCR. Subsequently, the index PCR was performed on the tagged 16S rRNA amplicons to barcode all samples with i7/adapter 1 and i5/adapter 2 indices. Paired-end sequencing was performed with the PCR products on an Illumina MiSeq resulting in two 300 bp reads.