GSM4655173: ESTn_flx_d13_2; Mus musculus; RNA-Seq
Eight samples per condition were fixated in QIAzol and kept at −80 °C until further use. Samples were homogenised using the QIAshredder and subsequently purified with the RNeasy microarray tissue kit according to manufacturer'sprotocol. The Qubit was used to determine the RNA concentration and the Bioanalyzer to determine the quality of the samples. Samples were further processed for Next Generation Sequencing according to the Illumina TruSeq Stranded mRNA protocol.