Identification reads (plasmid DNA) of ADAMTS1 plasmid
For each  promoter, barcoded TRIP plasmid library was transformed into DH5-alpha E. coli and plated; 100 colonies were picked, pooled, and expanded to produce plasmid mini-libraries with 100 different barcodes each. The barcodes in these mini-libraries were PCR amplified and sequenced by Illumina MiSeq to identify the barcodes belonging to each promoter.