GSM4476220: FL_FDC3; Homo sapiens; RNA-Seq
Tonsil tissues from 6 healthy donors and LN from 4 FL patients were collected and enzymatically dissociated. After depletion of hematopoietic cells (Dynal, Dynabeads CD45) three stromal cell subsets (FRC, FDC, DN) where sorted by FACS based on their phenotype and RNA extraction (Macherey-Nagel, NucleoSpin RNA XS) was performed. Quality control was performed using the Agilent RNA 6000 Pico Kit. RNA libraries were prepared (with 10ng of total RNA)  for sequencing using standard Illumina protocols