RNA-seq of human colon cancer cell line SW480 (36 h control)
Human colon cell line SW480 was cultured for 36 h after adherent. Total RNA was extracted using Trizol. The purity and integrity of RNA were detected by Nanodrop and Agligent 2100 respectively. A cDNA library was constructed as follows: The mRNA was enriched with Oligo (dT) magnetic beads, and then synthesized cDNA with random hexamers. Purified double-stranded cDNA was repaired at the end, and then an A-tail was added and ligated with a sequencing joint. The fragment size was selected using AMPure XP beads. The cDNA library obtained after PCR enrichment. The prepared DNA library was subjected to sequencing using an Illumina HiSeq platform.