Model S: 1 week after clindamycin
Extracted DNA (500 ng) was sheared to 200-300 bp using a E220 focused ultrasonicator (Covaris, U.K.) following manufacturers instructions. NEBNext Ultra DNA Library prep kit for Illumina was used for adaptor ligation and to PCR enrichment following manufacturers instructions. Libraries were sequenced using Illumina HiSeq 3000 sequencer.