GSM3462609: Pipecolic acid producer; Corynebacterium glutamicum; RNA-Seq
Harvesting procedure was done according to (Irla et al., 2015) and cell pellets were kept at -80 °C for further RNA isolation. The pellets were thawed in ice and RNA was isolated individually for each sample using NucleoSpin RNA isolation kit (Macherey-Nagel, Düren, Germany). RNA samples with genomic DNA contamination were treated with the RNase-free DNase set (Qiagen, Hilden, Germany) (Brito et al., 2017). The preparation and sequencing of the libraries were performed as described elsewhere (Irla et al., 2015; Mentz et al., 2013) The extracted RNA samples were pooled in equal parts and the pool of total RNA was subsequently used for the preparation of the cDNA libraries