Bacteria (16S) amplicon of JTNP Biocrust temoporal study
DNA extraction was performed with 0.15 g of biocrust using QIAGEN DNeasy PowerSoil kit (Qiagen, Germantown, MD, USA) following manufacture standard protocol. 515F and 806R primers set were used to amplify 16S V4 region for bacterial community following (Caporaso et al., 2011).