GSM3302494: B16-F0 #3; Mus musculus; RNA-Seq
Cell lines were trypsinized, washed with complete serum, centrifuged, and washed with PBS. Following washing cell pellets were frozen at -80C. After thawing, cells were resuspended in buffer RLT and lysed using QiaShredders. Extraction was performed using the Qiagen RNEasy Plus mini kit according to the manufacturer's protocol. Library construction was performed by Centrillion Genomic Services using the Illumina TruSeq RNA Sample Prep Kit