GSM4477852: E17.5 Lgr5-DTReGFP KO2 passage 4-Rspo3-100ng/ml_sample  84; Mus musculus; RNA-Seq
Total RNA was extracted with the miRNeasy Micro kit (Qiagen, Hilden, Germany) following the manufacturer's instructions. RNA libraries were prepared for sequencing using Ovation® SoLo RNA-Seq Library Preparation Kit (NuGEN, TECAN genomics). For organoids, RNA libraries were prepared for sequencing using the NEBNext rRNA depletion kit.