GSM3415639: BRCA1_MUT1; Homo sapiens; RNA-Seq
RNA from UWB1.289 (BRCA1 2594delC mutant; n = 3) and UWB1.289 BRCA1+ (BRCA1 WT; n = 3) cell lines was extracted using the RNA easy kit (Qiagen, Hilden, Germany). RNA quality was assessed using the Fragment Analyzer (Advances Analytical Technologies Inc., Ankeny, USA). RNA sequencing libraries were prepared using the Illumina TruSeq Stranded Total RNA reagents (Catalog number RS-122-2201; Illumina; San Diego, USA) according to the protocol supplied by the manufacturer and using 1ug of total RNA.