GSM4310184: TGF_beta_1_K27ac; Homo sapiens; ChIP-Seq
Cross linked with 1% formaldehyde for 10 mins followed by quinching with glycine and washed with TBS. ChIP-seq was performed as described by Zhong et al., 2017. ChIP-seq libraries were prepared from about 1 - 10 ng ChIP and input DNA using the ThruPLEX® DNA-seq Kit V2 (Rubicon Genomics, Ann Arbor, MI). The libraries were sequenced to 51 base pairs from both ends on an Illumina HiSeq 4000 instrument in the Mayo Clinic Center for Individualized Medicine Medical Genomics Facility.