GSM2978748: 422102-PRE; Homo sapiens; RNA-Seq
Blood was collected in EDTA tubes, and centrifuged. The buffy coat was collected and added to RNAlater. Total RNA was extracted from buffy coat samples using Qiagen RNeasy kits with an added homogenization step and on-column DNase digestion. The Illumina TruSeq Stranded Total RNA LT Sample Prep Kit (Cat#RS-122-2301) was used. RNA libraries were prepared for sequencing using standard Illumina protocols