RNA-Seq Phaeoacremonium minimum isolate Pm1119 in vitro rotating culture rep2
Pm. minimum isolate Pm1119 was grown in Czapek broth (pH 5.7) amended with 0.1% yeast extract and 0.1% malt extract at 25°C at 150 rpm in ambient light during 28 days. Fungal suspensions were vacuum-filtered through 1.6 µm glass microfiber filters (Whatman, Maidstone, UK). Total RNA was extracted from frozen ground mycelia using TRIzol reagent (Ambion, Austin, TX, USA) following the manufacturer's protocol. RNA-seq libraries were prepared using the Illumina TruSeq RNA sample preparation kit v.2 (Illumina, San Diego, CA, USA) and then sequenced on an Illumina HiSeq3000 sequencer (DNA Technologies Core Facility, University of California) in single-end 50-bp mode.