RNA-seq of Fragaria viridis: FVUPT-1
Using these short fragments as templates, random hexamer-primers were used to synthesise first-strand cDNA. Second-strand cDNA was synthesised using buffer, dNTPs, RNaseH and DNA polymerase I. Sequencing adapters were ligated to the short fragments after purification using a QIAquick Gel Extraction Kit. The adapters were used to distinguish different sequencing samples. Fragments were then separated by agarose gel electrophoresis and used as sequencing templates for polymerase chain reaction (PCR) amplification. Finally, the cDNA library was sequenced using an Illumina HiSeq 4000 system.