GSM4104541: Compound_D_in_H2O_Rep_3; Homo sapiens; RNA-Seq
Total RNA from Jurkat cells was extracted with Phenol-chloroform methods and quantified with an ND-1000 (Nanodrop). Samples were enriched in mRNA (with poly(A) tails) using oligo(dT) beads. After enrichment, the RNAs were fragmented into 250-400 bp fragments and the cDNA was synthesized with random hexamer primers and amplified