-seq of Lepidosiren paradoxa : adult buffy coat
Total RNA extraction from buffy coat  transcriptome was achieved using TRIzol Reagent (Life Technologies, Cat. 15596-026) according to the manufacturers protocol. RNA samples were further purified using RNeasy Mini Kit (Quiagen, Cat. 74104) and treated with DNaseI (Quiagen, Cat. 74254), according to the manufacturers protocol. RNA (0.5 g) was reverse transcribed using the SuperScript III First-Strand Synthesis SuperMix ( Life Technologies, Cat. 18080-400). In order to establish a buffy coat transcriptome reference, a HiSeq Illumina run was obtained from buffy coat library with 100 bp paired-end reads.