GSM3302629: 3his4wk_naive_pbs_3; Mus musculus; RNA-Seq
Mouse bladder were removed, flash froze in liquid nitrogen, RNA were extracted with QIAGEN RNeasy Plus kit (74136) Whole bladder total RNA was fragmented, depleted of genomic DNA, and dephosphorylated before ligating to DNA adaptors carrying 5'-AN8-3' barcodes with a 5' phosphate and a 3' blocking group. Barcoded RNAs were pooled and depleted for rRNA with the Ribo-Zero rRNA removal kit (Illumina MRZG12324), then synthesized corresponding cDNA libraries.