222 WGS staph pseudintermedius
Isolates subcultured twice on blood agar, grown in BHI for 8 hours, 1 mL aliquot used for extraction via MasterPure Gram Positive DNA Purification kit, standard protocol. DNA quantified using Qubit, normalized. Libraries prepared using Bioo Scientific NEXTflex Rapid DNA-Seq Kit, standard protocol. Sequenced with 300 x 300 cycle v3 sequencing kit.