GSM3417528: LPS_16h_RNAseq [DM4 control]; Mus musculus; RNA-Seq
Kidneys were snap frozen and pulverized under liquid nitrogen. Cryo-lysis was done using a Precellys tissue homogenizer in a polysome buffer consisting of Illumina TruSeq Polysome Buffer, Riboguard (Lucigen), Triton X-100, DTT, DNAse I, cycloheximide and NP40. Tissue lysates were centrifuged at 20,000 g for 10 min. Equal volumes of supernatant aliquots were made for ribo-seq and RNA-seq workflows. RNA libraries were prepared for sequencing using standard Illumina protocols and Ion Torrent protocols