GSM2303007: 000180Y; Mus musculus; RNA-Seq
Total RNA was prepared by using Sepasol-RNA I Super G (nacalai tesque) and 80 ng of total RNA was used for further steps. Isolation of poly(A)-tailed RNA and library construction were performed using Sureselect Strand Specific RNA Sample Prep Kit (Agilent Technologies). RNA libraries were prepared for sequencing using standard Illumina protocols