GSM3728400: P1A8_Pool_No_Dox_2; Homo sapiens; RNA-Seq
RNaesy- Qiagen kit-(#74106) was used for RNA extraction Concentrations of purified RNA were measured with the QuantiTTM RiboGreen® RNA Assay Kit  and normalized to 5 ng/μL. An automated variant of the Illumina TruSeqTM Stranded mRNA Sample Preparation Kit was used for library preparation from a 200 ng aliquot of RNA. This method preserves strand orientation of the RNA transcript and uses oligo dT beads to select mRNA from the total RNA sample. Following cDNA synthesis and enrichment, cDNA libraries were quantified with qPCR using the KAPA Library Quantification Kit for Illumina Sequencing Platforms and then pooled equimolarly.