GSM4476440: non-Tfh rep2 RNA-seq; Mus musculus; RNA-Seq
Sostdc1-EGFP-Tfh,Sostdc1-EGFP+Tfh,non-Tfh,Tfr and non-Tfr Treg cells were sorted,and RNA was harvested using Trizol reagent. RNA library preparation was conducted using an NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's protocols.CD4+CD44+ T cells were sorted and then analyzed by single cell RNA-seq.The scRNA-seq libraries were generated using Chromium single cell 3′ library and bead kit v.2 (10xgenomics) according to the manufacturer's protocol. RNA libraries were prepared for sequencing using standard Illumina protocols. Single cell RNA-seq libraries were generated using Chromium single cell 3′ library and bead kit v.2 (10xgenomics) according to the manufacturer's protocol.ATAC-seq DNA library was constructed using TruePrep DNA Library Prep Kit V2 for Illumina and TruePrep Index Kit V2 for Illumina.