GSM3669567: ProE_ERRBS_1; Homo sapiens; Bisulfite-Seq
Progenitor cells at each stage were sorted by a combination of cell surface markers for IL-3R, CD34 and CD36. Erythroblasts at each stage of terminal erythroid differentiation were sorted by a combination of cell surface markers for glycophorin A, band 3, and α4 integrin.  Genomic DNA was isolated using a Qiagen Gentra Puregene kit. Enhanced Reduced Representation Bisulfite sequencing (ERRBS) was performed at the Weill Cornell Genomics Core.