GSM4307188: splenic CD4+ T cells, KO_GFP_neg_replicate_2; Mus musculus; RNA-Seq
Total RNA was extracted with TRI reagent (Sigma-Aldrich, Munich, Germany). Depletion of the ribosomal RNA and library preparation was performed with the TruSeq® Stranded Total RNA Gold kit (former name TruSeq® Stranded Total RNA LT - (with Ribo-Zero TM GOLD), Illumina, San Diego, USA).