GSM2263337: MDAMB134_FAIRESeq_2; Homo sapiens; FAIRE-seq
Cells were fixed for 10 mins in 1% formaldehyde in FCS-free medium, washed and frozen. Nuclei were isolated and sonicated in Diagenode Bioruptor using 300ul volumes in 1.5ml eppendorfs. MDAMB134 (3x107 cells/tube) and T47D (1.5x107 cells/tube). Sonication was performed for 20 cycles of 30s on/off. Sonicate was subjected to three consecutive phenol–chloroform–isoamyl alcohol (25:24:1) extractions and reverse cross-linked overnight. DNA was purified by ethanol precipitation and quantified by Quanti-iT. Genomic DNA was isolated using Qiagen DNeasy Blood and tissue Kit according to protocol. DNA fragments are prepped for sequencing using the recommended protocol available for Angilent SureSelect-Illumina sequencing.