GSM2719461: AC-MR-3H-1; Aedes aegypti; ncRNA-Seq
Total RNA was extracted using Qiagen RNeasy mini kit according to manufacturer’s protocol. Quality of RNA was assessed using Bioanalyzer (Agilent, Santa Clara, CA, USA) according to manufacturer’s protocol. Quantity of RNA was analyzed using Qubit Fluorometer (Life Technologies, Grand Island, NY, USA) according to manufacturer’s specifications. Library preparation was conducted by the Notre Dame Genomics & Bioinformatics Core Facility using the Epicentre Scriptminer small RNA kit following the suppliers protocol. All steps of library construction and sequencing were performed at the Genomics Core Facility at University of Notre Dame. small RNA sequencing libraries. Sequencing of libraries was done by Illumnia MiSeq .