Rep-Seq of CAST/EiJ spleen
5' RACE first-strand cDNA synthesis was conducted on total RNA extracted from a section of spleen. Rearranged VDJ-IgM amplicons were generated using a single IgM oligo in the CH3 region of the mouse IGHM gene paired with a universal primer. Amplicons were used to generate SMRTbell template libraries and each library was sequenced across 2 SMRTcells on the RSII system using P6/C4 chemistry and 240 minute movies.