GSM4475893: CTR; Homo sapiens; RNA-Seq
Total RNAs were isolated from cells using Trizol (Invitrogen) followed by additional purification using an RNeasy Mini Kit (Qiagen, Valencia, CA) according to the manufacturer's instructions. Following our previous practice (PMID: 31849962), RNA-Seq library were prepared by the Genomics Core of West Virginia University using using KAPA mRNA HyperPrep Kit (KAPA Biosystems, Wilmington MA, USA) and sequenced by the Genomics Core of Marshall University using HiSeq 2500.