GSM3302016: IF_90d_13; Mus musculus; RNA-Seq
Brain tissue was homogenized in Trizol (Ambion) using a pellet pestle (Kontes), and total RNA extracted using phenol/chloroform separation and ethanol precipitation. Illumina TrueSeq RNA Library Prep Kit v2 was used to process mRNA. RNA libraries were prepared for sequencing using standard Illumina protocols