GSM3462379: FT190_shNTC; Homo sapiens; ATAC-seq
50,000 cells were washed in ice cold 1X PBS and re-suspended in 50 μl ice cold lysis buffer. Then nuclei were pelleted at 500 x g for 10 min at 40C. The pellet was then incubated with the transposition reaction mix for 30 min at 37°C and then purified using the MinElute PCR Purification kit (Qiagen, Hilden, Germany). ATAC-seq libraries were constructed as described in Bustos, et al. 2017 Genome Data (PMID: 28239551).