GSM2264212: Central Memory- Replicate 1; Homo sapiens; RNA-Seq
Peripheral whole blood was collected into BD Vacutainer tubes. Peripheral blood mononuclear cells were isolated using SepMate-50 tubes according to the manufacturer's supplied protocol (Stem Cell Technologies). CD4+ T cells were enriched using the EasySep Human CD4+ T cell enrichment kit and subsequently labeled with the BD Biosciences Naive/Memory T-cell panel (#561438) prior to fluorescence activated cell sorting. Total RNA isolated using TriReagent (Molecular Research Center) and purified with the RNeasy MinElute Cleanup kit (Qiagen) using an on-column DNAse treatment to ensure absence of genomic DNA contamination according to the manufacturer's supplied protocol. The 3026 project library was constructed using the Illumina TruSeq Stranded Total RNA Library Prep kit (Cat. No. RS-122-2201) following the manufacturer's supplied protocol.