GSM2977454: Sample_3-3S; Homo sapiens; ncRNA-Seq
Cells were lysated using TRIzol follow the company protocols. Total RNA were run in a 15% denaturizing gel with 7 M urea (0.8 ml 10x TBE, 1.4 ml water, 3.8 g urea, 3 ml acrylamide, 64 ul 10% APS, 4 ul TEMED). The region between 15 and 31 nt was cut and small RNA fraction was extracted from the gel using 0.3 M NaCl-TE overnight and purified using isopropanol. RNA libraries were prepared for sequencing using standard Illumina protocols.