GSM2877055: Growth media, biological replicate 1; Homo sapiens; OTHER
Bulk RNA from hESCs was extracted using Qiagen RNeasy Mini Kit. Single/multiple cells are FACS-sorted directly into the reverse transcription reaction mixture and lyzed by a freeze-thaw cycle (RNA was not purified). Adapted protocol from NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina® (provided with the BART-Seq method)