RNA-Seq of Heortia vitessoides: eggs, larvae, pupae, and adults
Total RNA was extracted using the E.Z.N.A.TM Total RNA Kit II (OMEGA Biotec, Norcross, GA, USA) following the suppliers instructions, and then treated with DNase I (Invitrogen, Life Technologies, Carlsbad, CA, USA). A Nanodrop 2000 spectrophotometer (NanoDrop Products, Wilmington, DE, USA) was used to check sample purity, while a Qubit1 2.0 fluorometer (Life Technologies, Gaithersburg, MD, USA) and Quantifluor-ST fluorometer with Agilent 2100 Bioanalyzer (Promega, Madison, WI, USA) were used to measure the concentration and integrity, respectively.