GSM4308399: 4364; Mus musculus; RNA-Seq
RNA samples were treated with DNase I (# 79254; QIAGEN) and assessed for quality using TapeStation 4200 (Agilent Technologies, Santa Clara, CA). All RNA samples had an RNA integrity number > 7.5. cDNA library preparation and sequencing were performed by the University of Michigan DNA Sequencing Core. Briefly, RNA samples were enriched for mRNA transcripts using NEBNext Poly(A) mRNA Magnetic Isolation Module (# E7490; New England BioLabs, Ipswich, MA). cDNA libraries were prepared from mRNA using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (# E7760; New England BioLabs). Libraries were assessed for quality and quantity using Agilent TapeStation and qPCR by KAPA Library Quantification Kit for Illumina Platforms (# KK4835; Kapa Biosystems, Wilmington, MA). Libraries were sequenced on the NovaSeq 6000 S4 Flow Cell (Illumina, San Diego, CA) for 200 cycles with 100 base pair paired-end reads.