GSM3132441: WT_DMSO_45min_repB; Saccharomyces cerevisiae; RNA-Seq
TruSeq RNA (stranded) RNA was extracted from frozen cell pellets by hot acidic phenol and alcohol precipitated. For all samples, rRNA was depleted by Yeast Ribo-Zero Gold. For all auxin and diazaborine experiments (including vehicle/untreated controls), libraries were prepared by TruSeq directional RNA kit by manufacturer's instructions. For diamide experiment, libraries were prepared by custom in-house protocol. RNA fragmented in alkaline buffer, size selected, treated with T4 PNK, 3' adapter ligated (with random hexamer), reverse transcribed with SuperScript III, circularized with CircLigase, PCR amplified with Phusion