GSM3208191: Eq6_S; Avicennia schaueriana; RNA-Seq
Samples were harvested under field conditions, stored in a RNA stabilizaing solution (RNAlater, Ambion) and RNA was extracted using the protocol developed by Oliveira et al., 2015 (DOI: http://dx.doi.org/10.4238/2015.December.28.32). Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 0.5 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols. Illumina barcode sequences: Eq4_L: CGATGT; Eq5_L: TGACCA; Eq6_L: ACAGTG; St1_L: GCCAAT; St2_L: CAGATC; St4_L: CTTGTA; Eq4_S: CGATGT; Eq5_S: TGACCA; Eq6_S: ACAGTG; St1_S: GCCAAT; St2_S: CAGATC; St4_S: CTTGTA; Eq4_F: CGATGT; Eq5_F: TGACCA; Eq6_F; ACAGTG; St1_F: GCCAAT; St2_F: CAGATC; St4_F: CTTGTA