Illumina sequencing of ZIKV_H/PFF_2013 stock
For the Zika-FP stock, we used an unbiased approach. This involved isolating vRNA, digesting any associated DNA with DNaseI, generating double stranded cDNA, fragmenting it into a library with the Nextera XT kit, tagging the library pieces, and then sequencing on the Illumina MiSeq.