GSM2877127: SIGAB1; Mus musculus; RNA-Seq
The femora, tibiae, and ilia of C57BL/6 mice of W41/W41 mice were crushed and the bone marrow cells were released and the red blood cells were lysed with ammonium chloride solution (STEMCELL Technologies). The cells were stained with the fluorophore-conjugated monoclonal antibodies, and sorted using an Influx cell sorter (BD Biosciences) equipped with five lasers. The cells were sorted into 1.5 ml tubes containing PBS + 2% FCS. Sorted cells were harvested, resuspended in 0.04% BSA (Thermo Fisher) and counted. Cells were processed using the 10x Chromium™ Single Cell 3' kit, following manufacturers instructions