GSM2366265: 221KO-1; Homo sapiens; RNA-Seq
Total RNA was extracted with TRIzol reagent (Life Technologies). 10 µg of RNA was used to enrich mRNAs with poly(A) tails using oligo(dT) Dynabeads (Life Technologies). The sequencing libraries were made using TruSeq Stranded Total RNA Library Prep Kit (Illumina). The quality of the libraries was verified by Agilent 2100 Bioanalyzer (Agilent).