Southwest carpet python virus-infected brain library
RNA was extracted from brain tissue from a SWCPV-infected carpet python (Morelia spilota). The RNA extract was depleted of ribosomal RNA (rRNA) using the RiboMinus Eukaryote System v2 (cat. no. A15026, Ambion, Thermo Fisher, Victoria, Australia) following the manufacturer s recommendations. The DNase-treated rRNA-depleted total nucleic acid extracts were then submitted to the Australian Genome Research Facility (www.agrf.org.au) for next-generation sequencing using Illumina MiSeq using 150 base pair, paired-end (PE) reads.