GSM4654869: WT31 induced 1 ChIP-seq; Homo sapiens; ChIP-Seq
ChIP and ChIP-seq were performed as described previously (22,23 from prev. version). Antibodies used were anti-ASCL1 (Abcam, cat# ab74065), anti-P300 (Santa-Cruz, cat# sc-585), and anti-IgG (Abcam, cat# ab6706). ChIP-seq experiments were performed in two separate clones of each of WT and S-A SH-SY5Y tetracycline-inducible stable cell lines induced with 1 μg/ml doxycycline for 24 hours, before cross-linking with 1% formaldehyde. ChIP-seq and the input libraries were prepared using the ThruPLEX® DNA-seq Kit (Rubicon Genomics, cat# R400406), following the manufacturers instructions.