GSM2720160: Neuroblastoma tumor TI_809 (seq); Homo sapiens; OTHER
Genomic DNA was extracted from cell lines and tumors using standard proteinase K and phenol-chloroform extraction protocol. DNA quality and quantity was assessed using a Nanodrop Spectrophotometer, PicoGreen and agarose gel electrophoresis. Amplicon library was prepared using GeneRead DNaseq Targeted HC Panel V2 (NGHS-501X-12, Qiagen) according to manufacturer instructions. DNA high-throughput sequencing was performed on Ion Proton instrument using Ion PI HiQ Sequencing Kit on the Ion PI sequencing chip (ThermoFisher Scientific)