GSM4156599: mouse brain (ATAC-Seq); Mus musculus; ATAC-seq
Female mouse dorsal skin was collected at first telogen (P21), anagen III (P28), late anagen (P32) and 2nd telogen (P55). The hair cycle stages were confirmed using cryosectioning. To dissociate skin cells, the skin samples were incubated in 0.25% collagenase in HBSS at 37°C for 35-45 minutes on an orbital shaker, followed by incubation in 0.25% trypsin-EDTA at 37°C for 35-45 minutes on the shaker. The skin samples were gently scraped from the dermal side. The single cell suspension was centrifuged for 5 minutes at 4°C, resuspended in 0.25% FBS in PBS and stained with fluorescent dye-conjugated antibodies and DAPI. Mouse brain were dissected, snap frozen on dry ice, and stored at -80 °C. The brain single nuclei suspension was prepared following OMNI-ATAC protocol. Mouse lungs were dissociated with fine scissors and then proteolytic digestion was performed using the Lung Dissociation kit (Miltenyi Biotech). Dissociated cells were then incubated at 37 °C for 20 minutes with rotation. Red blood cells were lysed using ACK buffer. Libraries are contructed using SHARE-seq procotol.