DNA-Seq of Pyxicephalus adspersus: adult female blood 3 kb mate-pair library
High molecular weight DNA was extracted from blood using a QIAGEN DNAeasy blood and tissue kit. DNA was fragmented and adapter tagged using tagment enzyme. Products were visualized in 1% agarose gels and size selected for 3 kb lengths using a QIAGEN Gel Extraction kit. Products were circularized, sheared, and sequencing libraries prepared and amplified following standard protocols and reagents in the Illumina Nextera Mate Pair Library kit.