GSM4407305: RCF2_2; Pyrus communis; RNA-Seq
Total RNA was isolated using Trizol reagent (Invitrogen, Carlsbad, CA, USA). The mRNA was purified from high-quality total RNAs using oligo (dT) magnetic beads and then broken into short fragments with fragmentation buffer. The cDNA was synthesized using a cDNA Synthesis Kit (TaKaRa, Dalian, China) and linked to sequencing adapters at both ends.