GSM2264769: H1 Ribominus eluate replicate 1; Homo sapiens; RNA-Seq
Both H1 and Hela cells were extracted as follows: 1.The media was removed; 2. The cells were washed twice with PBS; 3. TRIZol was added directly to the plate; 4. RNA was extracted by TRIZol; 5. The total RNA was treated with DNASe I; 6 The RNA was once again extracted by acid Phenol/Chloroform followed by Chloroform extraction; 7. The RNA was NaCl precipitated; 8. 80% ethanol washed twice; 9. Resuspended in Ultra pure H2O; 10. The desire amount of RNA was ribosome-depleted twice using a RiboMinus Kit. RNA-seq libraries were prepared according to Ilumina instructions for the TruSeq Stranded mRNA Kit and barcoded for sequencing.