   Illumina sequencing of H9 conditioned medium Methyl-seq library:  HpaII genomic fragments   
     Genomic DNA was extracted from sorted H9 human embryonic stem cells grown in conditioned medium (CM), and digested with HpaII.  Fragments were made into Illumina sequencing libraries by addition of Illumina linkers to repaired ends, PCR amplification, gel extration of fragments ~30-260bp (excluding linkers), and a second PCR amplification.  Purified DNA was used for cluster generation and sequencing using the Illumina 1G Genome Analyzer.     