RNA-Seq of Plutella xylostella: third instar larval hemocytes
Total RNAs of hemocytes from 200 parasitized P. xylostella at 24 h p.p were isolated. This group contains 3 biological replicates. A total amount of 1g qualified RNA per sample was used as input material for the library preparation. The sequencing libraries were generated using the VAHTS mRNA-seq v2 Library Prep Kit (Vazyme, Nanjing, China) for Illumina following manufacturers recommendations. The clustering of the index-coded samples was performed on a cBot Cluster Generation System (Illumina, USA) according to the manufacturers instructions. After cluster generation, the library preparations were sequenced on an Illumina Hiseq X Ten platform and 150bp paired-end module