16S rRNA amplicons from Pleurozium schreberi stems, Njallatjirelg, Sweden, new growth sections, pooled locations 1-6
Amplicons were generated with target-specific 16S rRNA V4 primers 515fB (GTGYCAGCMGCCGCGGTAA) and 806rB (GGACTACNVGGGTWTCTAAT), amended with Nextera XT adapter sequences on their 5' end (TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG for forward primer, GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG for reverse primer). Multiplexing was carried out in a two-step PCR approach with Nextera XT indices. Pooled amplicon libraries were sequenced on the Illumina MiSeq with the paired-end 300 bp kit.