GSM3417085: NF_LV5; Homo sapiens; RNA-Seq
Human ventricular myocardium was obtained from end-stage ischemic cardiomyopathic hearts explanted at the time of cardiac transplantation. Non-failing donor hearts that had been deemed unsuitable for transplantation were used as control. Prior to explant, hearts underwent intra-operative antegrade coronary perfusion with 4:1 blood cardioplegia solution. Following arrest, hearts were explanted and placed into cold Ca2+-free, modified Krebs-Henseleit solution as previously described (120). Samples were taken from mid-myocardial regions of the left ventricular free wall and the right ventricular free wall, in areas void of scar tissue. Tissue samples were rapidly frozen in liquid nitrogen and stored at -80°C until RNA isolation. Prospective written informed consent for research use of heart tissue was obtained from all transplant recipients or next of kin (non-failing donors). Patient consent, sample collection and preparation, and clinical data collection were performed according to a human subject research protocol approved by the Institutional Review Board of the Lewis Katz School of Medicine at Temple University (PI: Tsai). Sequencing libraries were generated using a NEBNext Ultra™ RNA Library Prep Kit for Illumina  following the manufacturer's recommendations