GSM3208854: Oncopeltus_fasciatus_ds-dnmt1_MethylC-seq_R2_Head_individual24; Oncopeltus fasciatus; Bisulfite-Seq
DNA methylation. MethylC-seq libraries were prepared using the protocol described in Urich et al. (2015; doi:10.1038/nprot.2014.114). Lambda phage DNA was used as a control for the sodium bisulfite non-conversion rate. Tissue was flash frozen in liquid nitrogen and stored at -80°C until processing. Total RNA and genomic DNA was extracted using a Qiagen RNAeasy micro kit (Qiagen, Venlo, The Netherlands) per manufacturer's instructions with Qiagen Qiazol as the lysis buffer. Complementary DNA (cDNA) was synthesized from 500 ng RNA with qScript cDNA SuperMix (Quanta Biosciences, Gaithersburg, MD).