GSM2367130: E16-2; Gallus gallus; RNA-Seq
Total RNAs were isolated by Trizol reagent (Invitrogen) and then treated with DNase (Promega) following the manufacturer’s instructions. Total RNA samples (5 μg) were treated with the ribo-zero-magnetic-kit (Epicenter) to remove rRNA, and then digested with 20 U of RNase R (RNR07250, Epicenter). RNA-seq libraries were prepared using the TruSeq® RNA LT Sample Prep Kit v2 (Illumina) following the manufacturer’s instructions.