GSM2366950: PolII_Pretreated-0.5h-MEF; Mus musculus; ChIP-Seq
Lisates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were constructed using a Mondrian SP (NuGEN Technologies Inc.) and the Ovation SP Ultralow Library system (NuGEN). Fragments ranging from 250 to 450 bp were selected for single-read sequencing on a HiSeq2000 (Illumina).