Single Cell RNA-seq of Human Kidney
Sorted cells were washed once with 0.04% BSA in PBS and counted on aby Countess II automated cell counter (ThermoFisher). 12,200 cells were loaded per lane on the 10x Chromium platform and processed for cDNA synthesis and library preparation as per manufacturer s protocol. Kidney 1 sample was processed usingby 10xChromium version 1 chemistry while Kidney 2 and Kidney 3 samples were processed by version 2 chemistry. cDNA and libraries were checked for quality on Agilent 4200 Tapestation and quantified by KAPA qPCR before sequencing on a single lane of a HiSeq4000