GSM3209412: Oral mucosa CD4+ T cells, CCR5+ CD69+; Homo sapiens; RNA-Seq
Cells were loaded onto a 10X Genomics GemCode Single-Cell Instrument per manufacturer instructions for nanodroplet single-cell capture, cell lysis, mRNA rtPCR, and cDNA barcoding within droplets. Per manufacturer instructions, droplet emulsions were broken, cDNA was amplified, and libraries were generated using enzymatic fragmentation.