GSM2723128: H9B_-CMC; Homo sapiens; OTHER
Total RNA was isolated from hPSC derived CNS neurons using the RNeasy Mini Kit following manufacturer’s protocol (Qiagen) Pseduo-seq libraries were prepared as described (Carlile et al. Nature 2014) Briefly, total RNA was fragmented, and was subsequently derivatized with CMC followed by reversal of CMC adducts. Fragments were size selected, followed by 3′ adapter ligation, and reverse transcription. Truncated RT fragents were size selected, circularized, and PCR amplified. PCR products were gel purified, and sequenced on an Illumina HiSeq 2000.