GSM3210751: P7_219; Mus musculus; RNA-Seq
Single cells have been extracted from tissue using Neuronal Tissue Dissociation Kit (Miltenyi Biotec) for embryonic and early postnatal stages. Papain Dissociation System (Worthington) was used for the adult mice. cDNA was generated from FACS sorted single cells using Smart-seq2 protocol (Picelli et al. Nature Methods 2013). For some of the samples, ERCC spike-in RNA was added to the lysis buffer. Nextera XT DNA sample preparation index kit, 96 indices (Illumina)