t1_TPII_100_2
The isolated plasmid pool samples were fragmented and subjected to library preparation. Library preparation and sequencing was performed using the dedicated kits and the SOLiD4 sequencer (Life Technologies), respectively. For each sample, 20-25 million of 50 nucleotide long reads were generated. Primary data analysis was carried out with software provided by the supplier (base-calling). The 50 nucleotide long reads were analyzed, quality values for each nucleotide were determined using the CLC Bio Genomics Workbench 4.6 program.