GSM2977827: Widetype in BCS relicate3; Cryptococcus neoformans var. grubii H99; RNA-Seq
Cells were pelleted using bead beating tubes (Mo Bio) and lysed in a mini bead-beater (BioSpec). RNA was extracted using the RNeasy Mini Kit (Qiagen). RNA was eluted in 50 µl of RNAse free water, and DNAse treated using Turbo DNAse (Roche) as suggested by the manufacturer. RNA samples for construction of the sequencing libraries for RNA-seq analysis were prepared using a TruSeq RNA sample preparation kit (Illumina) as standard Illumina protocols. The cDNA libraries were sequenced on an Illumina HiSeq 2000 instrument (Illumina). Approximately 0.9 - 1.8 × 107 paired-end, 75-bp reads were generated for each sample.