Draft Genome Sequences of an Extraintestinal Pathogenic Escherichia coli
Genomic DNA of strain ECS5 was extracted using a DNeasy blood and tissue kit (Qiagen, Germany) in accordance with the manufacturers instructions. Genomic DNA purity and concentration were evaluated by agarose gel electrophoresis and measured by using a fluorometer (Qubit; ThermoFisher).The DNA library was prepared using a Nextera XT DNA library preparation kit (Illumina, Inc., Cambridge, UK), and genomic DNA was sequenced on an Illumina HiSeq 4000 instrument with a 150-bp paired-end approach at a depth of approximately 200.