GSM4655327: GC054861_10X_RNAseq; Mus musculus; RNA-Seq
Cells at Day 16 of reprogramming were washed twice  with PBS without Ca/Mg, detached using 0.25%Trypsin incubated at 37C for 5 minutes; cells were resupsended in 2ml 0.04%BSA/PBS at the concetration of 1 mln cells/ml To obtain single-cell suspensions, cells at Day 16 of reprogramming were washed twice  with PBS without Ca/Mg, detached using 0.25%Trypsin incubated at 37C for 5 minutes; cells were resupsended in 2ml 0.04%BSA/PBS at the concetration of 1 mln cells/ml.  Single-cell suspensions were converted to barcoded scRNA-seq libraries by using the Chromium Single Cell 3' Library, Gel Bead & Multiplex Kit and Chip Kit (10x Genomics). Samples were processed using kits pertaining to Chromium V2 chemistry of 10X genomics.  Libraries were sequenced using HiSeq 4000 in paired-end mode.