GSM2107334: N2102362 estradiol rep4; Arabidopsis thaliana; RNA-Seq
Five rosettes per genotype per treatment were pooled and frozen into liquid nitrogen. Three biological replicates of RNA were isolated with RNA was isolated with a GeneJET Plant RNA Purification Mini Kit (Thermo Scientific). Quality and quantity of RNA was assessed using Agilent 2100 Bioanalyzer and NanoDrop. Libraries were prepared from 100 ng of total RNA with Illumina TruSeq RNA Sample Prep Kit v2 according to the kit manual.