GSM4658155: p10_cultured_2; Homo sapiens; RNA-Seq
Uncultured cells were extracted from PDX tumors. Cultured cells were harvested after 2 weeks in culture. Spheres were dissociated to a single cell suspension using dispase and trypsin. Uncultured and cultured cells were stained for FACS using human-specific CD298 and mouse-specific MHCI antibodies. RNA libraries were prepared for sequencing using standard Illumina protocols.