GSM3667342: Scla_Ribo_L1; Streptomyces clavuligerus ATCC 27064; RNA-Seq
Lysate for all samples were prepared by resuspended at polysome buffer and grinding in liquid nitrogen RNA-seq libraries were constructed with TruSeq Stranded mRNA LT Sample Prep Kit (Illumina) according to the manufacturer's instructions. Ribosome profiling libraries were additionally treated with 20 μM thiostrepton. dRNA-seq libraries were treated with RNA 5'-polyphosphatase (TAP) or none (N).