HiSeq X Ten paired end sequencing; Whole genome sequence (VarGoats 266 dataset)
Next Generation sequencing libraries are prepared using Illumina SeqLab specific TruSeq Nano High Throughput library preparation kits in conjunction with the Hamilton MicroLab STAR and Clarity LIMS X Edition. The gDNA samples are normalised to the concentration and volume required for the Illumina TruSeq Nano library preparation kits, then sheared to a 450bp mean insert size using a Covaris LE220 focused-ultrasonicator. The inserts are ligated with blunt ended, Atailed, size selected, TruSeq adapters and enriched using 8 cycles of PCR amplification. The libraries are evaluated for mean peak size and quantity using the Caliper GX Touch with a HT DNA 1k/12K/HI SENS LabChip and HT DNA HI SENS Reagent Kit. The libraries are normalised to 5nM using the GX data and the actual concentration was established using a Roche LightCycler 480 and a Kapa Illumina Library Quantification kit and Standards.