GSM2546210: Uninjured cardiomyocytes rep3; Danio rerio; RNA-Seq
Ventricle apexes were removed and cleared of injury tissue, then dissociated using the Miltenyi neonate heart dissociation kit, pelleted and resuspended in PBS/serum. Cell suspensions were strained on a 50 micron nylon mesh and immediately FACS-sorted directly into RLT lysis buffer (RNeasy micro-prep RNA extraction kit, Qiagen. RNA samples were prepared according to Qiagen protocol, cDNA libraries were generated and sequenced on an Illumina HiSeq 2000 RNA libraries were prepared for sequencing using standard Illumina protocols.