GSM4477738: Sox17ChIP_St10.5_Rep2; Xenopus tropicalis; ChIP-Seq
Chromatin for ChIP-seq was obtained after formaldehyde fixation of embryos and sonication as previously described (Chiu et al., 2014) using custom rabbit peptide anti-Sox17-C-terminal antibody (affinity purified by Bethyl labs). ChIP-seq libraries were generated using Bioo Scientific Nextflex ChIP-seq library preparation kit  (Catalog #NOVA-5143-01) and Nextflex barcodes (Catalog #51410).