GSM4473623: CAP_Bt1; Mus musculus; RNA-Seq
oocytes, cumulus cells, or blastocysts were transferred into the lysis buffer and directly reverse transcribed. A poly(A) tail was added to the 3′ end of the first-stand cDNA using terminal deoxynucleotidyl transferase. The total cDNA library was used for RNA-seq library construction after 18–20 cyclic amplification. Paired-end 125-bp sequencing was further performed