GSM3462563: stage III CRC patient_unfavorable_IonXpress_017; Homo sapiens; RNA-Seq
Total RNA was isolated from frozen CRC specimens. Tissues samples were homogenized with an Ultraturrax in 1ml of TRI Reagent® (Invitrogen). RNA was purified by RiboPureTM Kit according to the manufacturer's protocol (Invitrogen) and treated with RNase-free DNase. RNA was quantified by Qubit™ 3.0 Fluorometer (Invitrogen). After quantification total RNA was analyzed to determine the quality of samples by the Agilent RNA 6000 Nano LabChip® kit with the Agilent 2100 Bioanalyzer (Agilent Technologies). The accepted values of RNA integrity were between 5 and 8. Libraries for sequencing were prepared according to Ion Proton System's instructions