GSM2720133: pH 6.3_8h; Homo sapiens; RNA-Seq
TRIzol extraction (Invitrogen) according to the manufacturer's instructions. Media was aspirated from cells growing in 35 mm dishes. 1 mL of TRIzol Reagent was added to plates. A cell scraper was then used to collect cells and lysate which was frozen at -80 until RNA isolation following the manufacturer’s instructions with substitution of 1-Bromo-3-chloropropane for chloroform. RNA integrity was verified by bioanalyzer (Agilent Technologies) (RNA integrity number (RIN) 8.7-10.0, median = 9.7). Illumina TruSeq Stranded mRNA Library Preparation kit