GSM3462655: Spleen_M1 (mouse_BS); Mus musculus; Bisulfite-Seq
Total RNA was isolated from tissues or cultured cells using chloroform and isopropanol following the manufacturer's protocol. Polyadenylated RNA was separated from total RNA using either GenElute mRNA miniprepKit (Sigma-Aldrich) or Oligo dT Magnetic Beads (Vazyme). Bisulfite treatment was performed using the EZ RNA methylation kit (Zymo Research) with significant modifications.