CENP-A ChIP-seq: IP sample for 04T036 LSC cell line
Cross-linked ChIP experiments were performed with an anti-CENP-A polyclonal antibody using the MAGnify chromatin immunoprecipitation system (Life Technologies, Monza, Italy). Purified CENP-A immunoprecipitated (IP) and input samples were processed for library preparation using the TruSeq ChIP Library Preparation Kit (Illumina, San Diego, CA, USA ) and sequenced on the Illumina HiSeq 2500 platform, in a single-end 100-cycle run, with 140M reads/sample.