GSM3302183: NCCIT Teton-shOCT4 DOX (+) OCT4 S236D rescue; Homo sapiens; RNA-Seq
Total RNAs were extracted from germ cell cancer of Homo sapiens. Poly(A) mRNA isolation from total RNA (2 µg) and fragmentation were performed using the Illumina Truseq Stranded mRNA LT Sample Prep Kit with poly-T oligo-attached magnetic beads, according to the manufacturer's instructions. Libraries were prepared for multiplex sequencing using Illumina Truseq Stranded mRNA LT Sample Prep Kit, according to the manufacturer's protocol.