GSM3417859: HepG2_16hrs 0.5% O2_NA_Rep 1; Homo sapiens; RNA-Seq
Total RNA was prepared using the mirVana miRNA Isolation Kit (Ambion; Life Technologies Ltd, Paisley, UK) and treated with DNaseI (TURBO DNA‐free, Ambion). PolyA+ RNA libraries were then prepared using the ScriptSeq v2 RNA‐Seq kit (Epicentre, Madison, WI, USA). All RNA-seq experiments were performed in triplicate in accordance with ENCODE consortium guidelines