GSM4656412: Daedalus_WT1_MPP; Mus musculus; RNA-Seq
Purified populations of haematopoietic progenitors were spun down in FACS medium, washed and RNA was extracted. Purified RNA was obtained using QIAGEN RNeasy Micro kit or Zymo-research Direct-zol RNA Microprep kit. mRNA for RNAseq analysis was purified using PolyA+ selection and processed by the Yale Center for Genome Analysis using standard methodology using Illumina TruSeq mRNA-Seq Sample Prep Kits, library quality and concentration assayed using a Bioanalyzer, RNA was sequenced on a HiSeq2500 with 75bp pair-ended reads.