HAT-seq bulk sequencing of human tissues
Multiplexed HAT-seq libraries were constructed using KAPA LTP Library Preparation Kit (KAPA Biosystems, KK8232) with substantial modifications. Briefly, genomic DNA  consisting of ACC2 gDNA spiked with 1%, 0.1%, or 0.01% of ACC1 gDNA were sonicated and constructed into WGS libraries without size selection. 20 ng adaptor ligated DNA from each WGS library were amplified with one primer specific to L1Hs (L1Hs-AC-28) and another primer annealing to adaptor. Enriched libraries were barcoded by additional five cycles of PCR and size selected with Pippin Prep (Sage Science). Pooled HAT-seq libraries were paired-end sequenced (2*150bp) at Novogene, Inc (Beijing, China).