M. leprae Ml2-10
DNA was extracted from ethanol-fixed skin biopsy specimens. The biopsy was first digested using collagenase/dispase (Roche, Switzerland) and trypsin (AppliChem, Germany) and DNA was extracted with the QIAmp microbiome kit (Qiagen, Netherland) using an adapted method. Briefly, after human cell lysis and DNase treatment, bacilli were lysed with a mixture of 20 mg/ml of proteinase K (Qiagen, Netherland) and lysozyme (Sigma) prior to DNA purification on silica-based columns. DNA samples were subsequently sonicated to 400 bp-long fragments using the S220 Covaris instruments. Illumina libraries were prepared using the Kapa Hyper Prep kit (Kapa Biosystem, MA, USA) with the PentAdpters (Pentabases, Danemark) indexed adapters. After quality control (library concentration and fragment analyzer), libraries were multiplexed and sequenced as 100 base-long single-end reads on an Illumina HiSeq 2500 instrument.