NextSeq 500 sequencing; A PP2A-Pab1-mediated crosstalk between TORC1 & TORC2 regulates the differentiation response in fission yeast
A PP2A-Pab1-mediated crosstalk between TORC1 & TORC2 regulates the differentiation response in fission yeast
Cells were grown at temperature of 25C EMM (Moreno et al. Methods Enzymology 194: 795) containing NH4Cl as a nitrogen source.  Cells were washed with EMM containing no NH4Cl (EMM-N), resuspended in EMM-N, and grown at 25C for 240 minutes. Total RNA preparation was performed with MasterPureTM Yeast RNA Purification Kit (Epicentre) following the manufacturers instructions. Libraries were generated using TruSeq Stranded mRNA Library Prep Kit according to the manufacturers instructions