GSM4039767: vp14_FKBP5_HeLa_s10a_Upstream_TA; Homo sapiens; OTHER
The cells were fixed with formaldehyde and then lysised. Isolated nuclei were then fully digested by DpnII followed by overnight ligation. Ligated cycle DNA were purified after de-crosslinking incubation with proteinase K at 65 degree for 4 hours, followed by further digestion by NlaIII.  The digested DNA were ligated again and purified. The 4C library was  amplified with the viewpoint specific primers with inverse PCR. For each viewpoint, 8 PCR reactions products were pooled to enhance the library complexity. Kapa hyperprep kit