GSM4476484: CD4+ T cells, WT-72h-4; Mus musculus; ATAC-seq
Nuclei were incubated in transposition mix (TD reaction buffer, Nextera Tn5 Transposase [Illumina] at 37oC for 30min and then purified with MinElute PCR purification (Qiagen) Libraries were prepared with the Nextera XT DNA Library Preparation Kit (cat# FC-131-1001) according to manufacturer instructions and then purified with MinElute PCR purification (Qiagen).