AB SOLiD 3 Plus System sequencing; Transcriptome profile of Herbaspirillum seropedicae SmR1 grown in the presence of aromatic compounds
Transcriptome profile of Herbaspirillum seropedicae SmR1 grown in the presence of aromatic compounds
Herbaspirillum seropedicae SmR1 was grown in NFbHPN medium until OD600nm of 0.6, when 5mM phenol or 2,5mM benzoic acid was added to the medium. After 30 minutes of growth, the cells were collected by centrifugation and total RNA was extracted with RiboPureBacteria. RiboPure Bacteria (Ambion) After rRNA depletion 500 ng of depleted rRNA was used to construct the sequencing libraries following standard protocols using the SOLiD Total RNA-Seq Kit (Life Technologies). The libraries were barcoded by using the SOLiD Transcriptome Multiplexing Kit (Life Technologies).