GSM3131810: cerebellum_Control_3; Mus musculus; RNA-Seq
Brain was extracted from each mouse and the whole striatum and cerebellum were dissected, snap frozen on dry ice and stored in a centrifuge tube at -80°C following proven protocols (Gibbons et al. 2014). Tissue from each mouse was homogenized with an RNase-free disposable pellet pestle (Fisher) and RNA was extracted using the commercially available RNeasy® Lipid Tissue Mini Kit (Qiagen, Valencia, CA). Purification of the isolated RNA included treatment with DNase I (Qiagen, Valencia, CA). RNA libraries were prepared for sequencing using standard Illumina protocols