RNA-seq of Homo sapiens: brain chroroid plexus Alzheimers Disease symptomatic
Total RNA was isolated using QIAsymphony RNA Kit (Qiagen) according to the manufacturers instruction. The RIN (RNA integrity number) was evaluated to check quality. The library was constructed with TruSeq Stranded Total RNA Ribo-Zero Library Preparation Kit Illumina and the paired end sequencing was performed with the HiSeq 2500 (Illumina Inc., San Diego, CA, USA). Quality control were performed using FASTQC (v.0.11.2) to check quality sequence in 42 sequenced samples. Sequences were trimmed to eliminate poor base quality and adaptor contamination using FASTX (v.0.0.13). Sequences were mapped in reference Genome Reference Consortium Human build 38 (GRCh38) using HISAT2 (v 2.1.0)