GSM4475084: K562_4xAP3_r1; Homo sapiens; OTHER
Genomic DNA was isolated using the Bioline Isolate II Genomic DNA Kit. The full protocol is described in Leemans, 2019. 500 ng of genomic DNA was digested at Dam-methylated GATC sequences with DpnI and ligated to adapters. Following DpnII digestion of intact GATC sequences, DNA fragments were amplified with 22-26 PCR cycles. Amplified fragments were further processed with end-repair, 3A-overhang tailing and Y-shaped adapter ligation, before a final round of 9-12 PCR cycles with illumina index adapters.