Bacteria (16S) amplicon of JTNP Biocrust temoporal study
DNA extraction was performed with 0.15 g of biocrust using QIAGEN DNeasy PowerSoil kit (Qiagen, Germantown, MD, USA) following manufacture standard protocol. ITS1F and ITS2 primers set were used to amplify the internal transcribed spacer region (ITS) for fungal community according to Smith and Peays Illumina Miseq protocol (Smith and Peay, 2014). 515F and 806R primers set were used to amplify 16S V4 region for bacterial community following (Caporaso et al., 2011).