RNAseq of Mtb infected lung: anti-IL-17
DNase-treated total RNA was isolated from lung tissue using Rneasy RNA isolation kit. Each Sample was assessed using Qubit 2.0 fluorometer and Agilent Tapestation 2200. Sequencing libraries were generated using Illumina TruSeq RNA Access library prep kit. Cluster generation and 75 bp paired-read dual-indexed sequencing was performed on the Illumina NextSeq 500 platform. RNA seq analysis was performed using the Maverix Analystic Platform.