GSM4655132: N1 DMBA HFD Liver RNA; Mus musculus; RNA-Seq
RNA was isolated from the liver using TRIZOL and purified on RNeasy spin columns. The purity was checked with Agilent Tapestation. Libraries were prepared according to Illumina's TruSeq Stranded mRNA library preparation kit protocol. The cleaned adapter ligated libraries were pooled and loaded on the NextSeq 500 with a 75 cycle sequencing module to generate paired-end reads (43bp+43bp+6bp).