Illumina HiSeq 2000 sequencing; Alu element-containing RNAs maintain nucleolar structure and function
Alu element-containing RNAs maintain nucleolar structure and function
Cells were grown in DMEM medium containing 10% FCS, at 37 degree C  and 5% CO2 supply. Cells were transfected for 14 hours with transfection reagent only (Mock), with a control antisense oligonucleotide (ControlASO) or with an antisense oligonucleotide targeting alu-repeat containing RNA transcripts (AluASO). RNA was extracted from whole cells or cellular fractions (nucleoplasmic = nuclear minus nucleoli; nucleoli = isolated nucleoli) using the RNeasy Mini Kit from Qiagen. Libraries were prepared using the NEB library prep kit for long RNAs.