small RNA of Zebrafish: zygotes 0hpf
Next Flex small RNA-seq kit v3, from BIOO scientific, starting from 1ng of total RNA. Followed the protocol version  V16.06, diluting the adaptors  for ligation and using 25 PCR cycles for library amplification. The library was size selected using an 8% TBE gel, and the library fragments of 146-168bp (corresponding to insert sizes of 18-40bp) were recovered and purified.