GSM2107228: M27Liv; Mus musculus; RNA-Seq
Livers were collected after removing the gallbladder. Intestinal contents were flushed with phosphate buffered saline and intestinal tissues were separated into various sections, namely duodenum, jejunum, ileum, and large intestine (colon).  All tissues were snap-frozen in liquid nitrogen and stored at -80˚C.Total RNA was isolated from tissues using RNA Bee reagent and RNA integrity was confirmed using a dual Agilent 2100 Bioanalyzer (RIN>7.0). The cDNA libraries from total RNA samples were prepared using an Illumina TruSeq RNA sample prep kit. Three micrograms of total RNA were used as the RNA input according to recommendations of the manufacturer’s protocol.