Whole oyster tissue Miseq PE 250: Juvenile
Amplicons were performed on a paired-end Illumina MiSeq platform to generate 300bp paired-end raw reads, and then pretreated. Specific processing steps are as follows: 1) Paired-end reads were assigned to a sample by their unique barcode, and the barcode and primer sequence were then truncated. 2) Paired-end reads were merged using FLASH (V1.2.7,http://ccb.jhu.edu/software/FLASH/ ) ,a very fast and accurate analysis tool to merge pairs of reads when the original DNA fragments are shorter than twice of the reads length. The obtained splicing sequences were called raw tags.