RNA-Seq of Homo sapiens: adjacent noncancerous cervix tissues
Total RNA was extracted from 10 to 20 mg of tissue with the RNeasy Mini Kit (Qiagen, Hilden, Germany). The RNA integrity of each sample was assessed using agarose gel electrophoresis. The concentration of total RNA was determined by NanoDrop 2000 (Thermo Fisher Scientfic, Wilmington, DE). The full transcriptome sequencing libraries were prepared using the NEBNext Ultra RNA Directional Library Prep Kit (NEB#420) according to the manufacturers instructions.