24h heat treatment of GHA in Chinese Cabbage
Messenger RNAs (mRNAs) from the total RNA were isolated using Oligo (dT) and were randomly   cleaved into short fragments. Then the first strand cDNAs were synthesized with random   hexamer primers and followed by second strand cDNAs synthesis using DNA polymeraseⅠ(New   England BioLabs) and RNase H (Invitrogen). After end repair, adaptor ligation, and index   codes adding for each sample, PCR amplification was performed. The qualified cDNA library   was carried out by an Illumina HiSeq 2000 platform.