GSM4476228: CD44 KO Hs578T rep 2; Homo sapiens; RNA-Seq
RNA was harvested using the RNeasy Mini Kit (Qiagen #74104) and 2000ng RNA was submitted per sample. Twelve dual-indexed Illumina TruSeq stranded mRNA libraries were pooled for sequencing on 1 lane of a HiSeq 2500 using v4 chemistry to generate 2x50 bp paired-end reads. RNA libraries were prepared for sequencing using standard Illumina protocols.