Paired end Illumina sequencing of Pantoea ananatis OC5a
Bacteria were cultured overnight in 5 ml LB broth at 28C with shaking. Cells were pelleted and lysed using standard methods. DNA was extracted from the sample and purified using standard methods. DNA was quantified by PicoGreen and a Nanodrop instrument. The DNA library was prepared using the NEBNext Ultra II FS DNA library preparation kit for Illumina with NEBNext multiplex oligonucleotides (New England Biolabs, USA). AMPure XP beads (Beckman Coulter, USA) were used for DNA size selection and purification. The DNA library was sequenced on a MiSeq instrument (Illumina, USA) with the 2 (paired-end) x 250-bp mode. Further details of the DNA isolation and sequencing will be presented in a future publication.