Ion Torrent PGM sequencing; Effect of a KRAS peptide nucleic acid clamp on the Ion Torrent sequencing protocol
Effect of a KRAS peptide nucleic acid clamp on the Ion Torrent sequencing protocol
The relevant PNA clamps were added at a concentration of 10 µM per PNA clamp, before the initial multiplex PCR amplification with the primer pool. Library generation followed the protocol described in the Ion AmpliSeq Library Kit 2.0 User Guide using the Ion AmpliSeq Cancer Hotspot Panel v2 primer pool, comprising 207 amplicons covering approximately 2,800 COSMIC mutations from 50 oncogenes and tumor suppressor genes.