NextSeq 500 sequencing; Elucidation of the mechanism behind the potentiating activity of baicalin against Burkholderia cepacia complex biofilms
Elucidation of the mechanism behind the potentiating activity of baicalin against Burkholderia cepacia complex biofilms
24hour old biofilms treated for 24hours in 96 well plate harvested by two cycles of vortexing (5min) and sonicating (5min)on ice, collected in falcon tubes 96 well plates, approximately 10^8 cells per well Starting from a fresh culture, 4hours adhesion rinsing of the biofilm 20hours maturation 24 hour treatment with - physiological saline (control) - baicalin hydrate (250M)in physiological saline - tobramycin (768g/ml) in physiological saline - baicalin hydrate (250M) + tobramycin (768g/ml) in physiological saline Ambion RiboPure Bacteria Kit Truseq stranded Total RNA library prep