GSM3728928: RNAseq_A431_shUSP28_rep2; Homo sapiens; RNA-Seq
RNA was isolated using ReliaPrep™ RNA Cell Miniprep System Promega kit, following the manufacturer's instruction manual. mRNA was purified with NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB) and the library was generated using the NEBNext® UltraTM RNA Library Prep Kit for Illumina, following the manufacturer's instructions. For the size-selection of the libraries, Agencourt AMPure XP Beads (Beckman Coulter) were used. Library quantification and size determination was performed using Fragment Analyzer (Agilent formerly Advanced Analytical). Libraries were sequenced on an Illumina NextSeq500.