GSM2877031: RNA-seq; Saccharomyces cerevisiae; RNA-Seq
Ribosome profiling was performed as previously described [PMID:19213877; PMID:22836135], with minor modifications. For depleting rRNA we utilized Ribo-Zero by EpiCentre. We utilized the small RNA sequencing kit from New England BioLabs. Sucrose gradient fractionation was employed for filtering reads with one or two ribosomes. Additional filtering of DNA with the relevant length before sequencing was performed with BluePippin.