Ion Torrent PGM 100bp Whole Gneome Shotgun Sequencing of E.coli Sakai
The 100bp sequencing of the Sakai strain were done with Bioruptor shearing, 316 chip, and TSS v1.5.2. Quality control analyses for the library were performed with a Caliper Labchip GX using the DNA High Sensitivity Assay (Caliper Life Sciences, Mainz, Germany). Library concentrations were determined with the Qubit dsDNA HS Assay Kit (Life Technologies).