GSM3211240: Liver_L_044; Mus musculus; RNA-Seq
Single cell RNA sequencing was performed as previously described (Chu L-F. et al 2016 DOI:https://doi.org/10.1186/s13059-016-1033-x  ) with the following modifications. In this study, we used small (5-10 μm), medium (10-17 μm), and large (17-25 μm) plate sizes. ERCC RNA Spike-In (ThermoFisher Cat. No. 4456740) was diluted in the lysis mix following the manufacturer's user guide and previous studies (Brennecke P. et al. 2013 DOI:https://doi.org/10.1038/nmeth.2645). Library construction was performed as previously described (Chu L-F. et al 2016 DOI:https://doi.org/10.1186/s13059-016-1033-x)