GSM2804221: induced iSLK.219 cells m6A IP rep 1; Homo sapiens; RIP-Seq
RNA was isolated and DNase treated as in the m6A RIP, except the RNA was first fragmented to lengths of ~100 nt prior to immunoprecipitation with anti-m6A antibody (Synaptic Systems). Immunoprecipitated RNA fragments and comparable amounts of input were subjected to first-strand cDNA synthesis using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs). Sequencing was carried out on Illumina HiSeq2500 according to the manufacturer’s instructions, using 10 pM template per sample for cluster generation, TruSeq SR Cluster kit v3 (Illumina), TruSeq SBS Kit v3-HS (Illumina) and TruSeq Multiplex Sequencing primer kit (Illumina).