GSM3415997: CTGATC_HmrI: small RNA library (18-29 nt); Drosophila melanogaster; ncRNA-Seq
Total RNA was isolated by Trizol (Invitrogene) and for small RNA libraries preparation were size-fractioned by PAGE (19-29 nt) Testes were dissected from adult flies (0-5 d) and RNA was isolated with Trizol Reagent (Invitrogene). For small RNA librarires, 19-29 nt RNAs were isolated from total RNA of dissected testes using 12% polyacrylamide gels and cloned as described in previous studies (Aravin et al., 2008; Brennecke et al., 2007). For Ribo-depleted libraries, rRNA was depleted with Ribo-Zero™ rRNA Removal Kit. Standard RNA-seq libraries were generated using the NEBNext Ultra Directional RNA Library Prep kit (NEB #E7420) according to the manufacturer's instructions.