GSM3728957: D21BC: Day 21 scrambled shRNA 1; Homo sapiens; RNA-Seq
On day of harvest, pellets were rinsed once in PBS, snap frozen in liquid nitrogen, and stored at -80℃ until further processing. On day or RNA isolation, pellets were homogenized in buffer RL using a bead beater (BioSpec Products) at 2,500 oscillations per minute for 20 seconds for a total of three times. Subsequent steps were performed following manufacter's protocol (Norgen total RNA isolation kit; Norgen Biotek). Libraries were prepared using TruSeq Stranded Total RNA with Ribo-Zero Gold Kit (Illumina)