GSM3728840: Hippocampal cells_C1-M; Mus musculus; RNA-Seq
Hippocampal total RNA was isolated using the miRVANA RNA isolation kit (ThermoFisher) RNA was quantified using the Qubit 2.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA) and quality was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies Inc., Palo Alto, CA) with the RNA 6000 Nano kit (Caliper Life Sciences, Mountain View, CA). Libraries were prepared, including rRNA reduction, using the ScriptSeq Complete Gold Kit (H/M/R) (Illumina Inc., San Diego, CA).