apolpp mutant fly replication 1 injected M. luteus
Total RNAs were extracted from treated w1118 and apolpp mutant at 6h post-injection using Trizol reagent (Invitrogen, USA) following the manufacturer's protocol. A total of 3 g RNA per sample was used for the RNA sample preparations. Sequencing libraries were constructed using NEBNext1 Ultra RNA Library Prep Kit for Illumina1 (NEB, USA). The libraries were sequenced on an Illumina Hiseq 4000 platform and 150bp paired-end reads were generated. The quality of the raw data was assessed using the FastQC and FastX-Toolkit.