GSM4473443: FUSCIP2; Homo sapiens; RIP-Seq
Total RNA from whole-cell inputs and RNAs co-IPed with FUS-R521C were extracted using TRIzol. The RNAs were fragmented by sonication and were captured using CU5T45 chimeric magnetic beads. The captured RNAs were then eluted from the beads and poly (A) tails removal by RNase H treatment. Eluted RNAs were then subjected to 5' and 3' adaptor ligation, RT-PCR and sequencing (Illumina TruSeq LT library preparation kit).