GSM3067297: BDI644: Heat Bio 2; Brachypodium distachyon; RNA-Seq
RNAs were isolated using Trizol reagent (Molecular Research Center). PARE libraries were constructed as described (German et al., 2008). RNA-seq libraries were constructed using the ScriptSeq RNA-Seq Library Preparation Kit from Illumina (Cat#SSV21124) and sequenced using Illumina HiSeq 2500.