Swanson_5kb_160nt
The library was constructed with the Nextera Mate Pair library Sample Prep kit (Illumina, CA), followed by the TruSeq DNA Sample Prep kit. Library insert size: 3kb - 5kb. The library was quantitated by qPCR, pooled with the other two mate-pair libraries and the 800bp shotgun library and sequenced on four lanes  for 161 cycles from each end of the fragments on a HiSeq2500 using a TruSeq SBS sequencing chemistry version 4. Fastq files were generated and demultiplexed with the bcl2fastq v1.8.4 Conversion Software (Illumina).