GSM2878894: Rpb1-AA_60min_Taf1; Saccharomyces cerevisiae; ChIP-Seq
Sheared chromatin (100 bp ~ 300 bp) was prepared using a Misonix sonicator 300 with cup horns. Crosslinked chromatin was precipitated by standard methods with specific antibodies. Libraries were prepared according to previous methods (Wong, K.H., Jin, Y., and Moqtaderi, Z. (2013). Multiplex Illumina sequencing using DNA barcoding. Curr Protoc Mol Biol Chapter 7,  unit7.11.–7.11.11.).