GSM2722575: Liver CNTRL 4-5; Danio rerio; miRNA-Seq
Isolation of total liver RNA was performed using TRIzol reagent (Invitrogen), and the extracted RNA were further purified using the miRNeasy Mini kit (Qiagen, Valencia, California). All RNA were treated with DNase to ensure no DNA appeared in the results. The miRNA-Seq libraries were prepared using with Illumina TruSeq Small RNA Prep kit and 1 ug input RNA