GSM2454456: + C4_X; Homo sapiens; RNA-Seq
The RNA isolation procedure was performed with the NucleoSpin RNA Kit according to the manufacturer’s instructions. Library preparation, comprising rRNA depletion, polyA enrichment, fragmentation to ~270 nucleotide length and reverse transcription to cDNA, using random hexamer primers and adapter ligation, was accomplished with the TruSeq RNA Sample Preparation Kit v2 according to the manufacturer’s protocol by EMBL GeneCore facility in Heidelberg (Dr. Vladimir Benes).