GSM2264175: Ncrassa_CHAP-Dam_DamID-seq; Neurospora crassa; OTHER
Whole-genome DamID-seq was performed using a procedure adapted from (Zhou, V.W., 2012)(48). Briefly, genomic DNA from the Dam-tagged CHAP strain was digested with DpnI. Digested DNA was ligated to adapters and amplified using a biotin-tagged primer. The amplified DNA was fragmented by sonication to 100-500 bp products and purified using streptavidin conjugated beads (Sigma). Bound DNA was eluted using a DpnII digestion. Purified DNA was prepared for sequencing using Illumina TruSeq ChIP-Seq Sample Prep Kit.