GSM3417034: TriplexDNASeq_rep3_minus_RNA_ligation; Homo sapiens; OTHER
Chromatin was purified and chromatin-associated nucleic acids were isolated. Following the ligation of a biotinylated linker oligonucleotide to the 3'-end of RNA, RNA and associated DNA complexes were captured on streptavidin beads. RNA-associated DNA was eluted and sequenced. DNA libraries from biological replicates were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) and NEBNext Multiplex Oligos for Illumina (NEB).