Illumina HiSeq 4000 paired end sequencing; RNAseq from endosperm and pericarp of 3 wheat genotypes: Cadenza, triple stack of TaiRX9b knock-out alleles, null segregant control
RNAseq from endosperm and pericarp of 3 wheat genotypes: Cadenza, triple stack of TaiRX9b knock-out alleles, null segregant control
endosperm isolation. rolled out from grain Glasshouse growth. 16h photoperiod 25/19 oC total RNA isolation. As in Pellny et al. (2012) Library Preparation_RNA-Seq PolyA. The mRNA fraction will be selected from the total RNA provided before conversion to cDNA. Second strand cDNA synthesis will incorporate dUTP. The cDNA will be end-repaired, A-tailed and adapter-ligated. Prior to amplification, samples will undergo uridine digestion. The prepared libraries will be size selected, multiplexed and QC'ed