GSM2366376: Control_1; Sus scrofa; ncRNA-Seq
The uterus was flushed with 10 ml and another 50 ml of phosphate-buffered saline (autoclaved, ph 7.4). Then, endometrial samples were collected, shock frozen in liquid nitrogen and stored at – 80 °C. RNA was harvested by means of TRIzol reagent. For preparation of the small RNA-Seq libraries, the NEBNext© Small RNA Sample Prep Set 1 (New England Biolabs, Frankfurt-Höchst, Germany) was utilized according to the manufacturer´s instructions.