RNA-Seq of Microphallus livelyi: metacercariae collected from Potamopyrgus antipodarum
We isolated Microphallus metcercariae from 4 Potamopyrgus antipodarum individuals collected from Lake Alexandrina and 4 Potamopyrgus antipodarum individuals collected from Lake Kaniere. Metacercariae were pooled and RNA was extracted using TRIzol. RNA quantity and quality were assessed with a Bio-Rad Experion Automated Electrophoresis Station, following manufacturer protocol, requiring an RQI >/= 8 and a minmum of 2ug of total RNA. We generated cDNA libraries following the Illumina TruSeq LS protocol, and then performed 2x100bp paired-end RNA sequencing on an Illumina HiSeq 2000.