GSM2262916: WT-2-H3K27me3; Oryza sativa Japonica Group; ChIP-Seq
About 2 g of seedling leaves were crosslinked in 1% formaldehyde under vacuum. Chromatin was extracted and fragmented to 200-750 bp by sonication, and ChIP was performed using the following antibodies: H3K27me3 (a2363, ABclonal) and anti-SDG711 (Liu et al., 2014). The precipitated samples were analyzed by high throughput sequencing. For genomic bisulfite sequencing, the total genomic DNA of callus culture- regenerated wild type (WT), SDG711 over-expression lines were prepared using the DNeasy plant mini kit (Qiagen). Genomic DNA (5 g) was used to generate BS-Seq libraries as previously described (Feng et al., 2011). Sequencing was performed at the Novogene Bioinformatics Technology Co. Ltd, China. DNA from ChIP was used to construct sequencing libraries following the protocol provided by Illumina TruSeq® ChIP Sample Prep Set A described in http://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqchip/truseq-chip-sample-prep-guide-15023092-b.pdf.