GSM4475892: WT_6_PairI [12I2Lardelli_2]; Danio rerio; RNA-Seq
Total RNA was extracted from pooled larvae using the mirVanaTM miRNA isolation Kit (Ambion, Life Technologies, Thermo Fisher Scientific, Waltham, MA, USA).  DNase treatment was performed on RNA to remove remaining genomic DNA using the DNA-freeTM Kit (Ambion, Life Technologies, Thermo Fisher Scientific, Waltham, MA, USA). PolyA amplification was performed for the construction of sequencing libraries. Demultiplexed libraries were provided by the sequencing centre at SAHMRI as 75bp single-end reads.