GSM2722230: RNAseq_Fibroblast_B; Homo sapiens; RNA-Seq
Total RNA from Day 30 miNs and starting human adult fibroblasts were extracted by column-based method (RNeasy plus micro kit, Qiagen). mRNA was isolated by using SMARTer Ultra Low RNA Kit (Clontech) for Illumina sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols.