GSM3302830: embryo4-2,1,2; Mus musculus; RNA-Seq
Embryos were dissociated to single cell suspensions by adding 100 uL of TrypLE (Invitrogen, #12605010) and pipetting the embryo or embryo pieces every 5 minutes for ~30 minutes until complete dissociation was visually confirmed.  Trypsin was deactivated by adding 100 uL  PBS+BSA is added to the droplet and moving cells into a 1.5 mL eppendorf tube, followed by several rounds of additional collection with 100 to 200 uL of PBS+BSA to a final volume of 1 mL. Single cells were lysed in droplets using the 10x Chromium instrument. 10X Genomics Chromium Prep v2