Laminaria digitata control experiment
Total RNA was extracted from 100 mg of frozen algal materials according to Apt et al. [16], treated with RNAse-free DNase I (Stratagene, La Jolla, CA, USA) to eliminate genomic DNA contamination, and quantified using a Nanodrop ND 1000 spectrophotometer (Labtech International Ltd, Lewes, UK). Four non-normalized cDNA libraries were constructed, by pooling total RNAs extracted from grazed or control sporophytes collected during the grazing kinetics after 6, 12, 24 and 48 hours, using the SMART cDNA library kit from Clontech (CA, USA). Differentially-labelled shot-gun cDNA libraries were sequenced using 4-5-4 technology at the Max Planck Institute of Berlin-Dahlem.