D. simulans w[XD1] small RNA replicate 1
For small RNA data, we extracted RNA from 7-day-old D. simulans  testes and accessory glands using Trizol (Invitrogen). 1 g of total RNA were used to prepare small RNA libraries as previously described (Lee and Yi, 2014) and sequenced on HiSeq2500 using SR50 at the New York Genome Center.