AB SOLiD 3 Plus System sequencing; Transcriptome profile of Herbaspirillum seropedicae SmR1 grown in the presence of aromatic compounds
Transcriptome profile of Herbaspirillum seropedicae SmR1 grown in the presence of aromatic compounds
Herbaspirillum seropedicae RP (derivative strain resistant to 1mM phenol) was grown in NFbHPN medium containing 1mM phenol until OD600nm of 0.6, then 5mM phenol was added to the medium. After 30 minutes of growth, the cells were collected by centrifugation and total RNA was extracted with RiboPureBacteria. RiboPure Bacteria (Ambion) After rRNA depletion 500 ng of depleted rRNA was used to construct the sequencing libraries following standard protocols using the SOLiD Total RNA-Seq Kit (Life Technologies). The libraries were barcoded by using the SOLiD Transcriptome Multiplexing Kit (Life Technologies).