GSM4258230: Cytosol non-infected mRNA, replicate 3; Homo sapiens; RNA-Seq
Total RNA was extracted by a hot-phenol protocol and mRNA isolated using the TruSeq stranded mRNA library prep kit (Illumina). For ribosome profiling lysates were treated with RNAse I and subjected to polysome profiling. RNA was collected from the monosome peak and size-selected using a denaturing urea gel. Ribosomal RNA was removed using the Ribo-Zero Gold rRNA Removal kit (Epicenter). RPF libraries were prepared using the protocol described in (Ingolia, 2009), (Nedialkova and Leidel, 2015) and (Jungfleisch et al. 2017). For RNA-seq libraries, the TruSeq stranded mRNA library prep kit (Illumina) was used.