GSM4307380: 14489X7 BPN C 2; Mus musculus; RNA-Seq
For bulk RNAseq, FACS-sorted cells were pelleted and total RNA was isolated using the Animal Tissue RNA Purification Kit (Norgen Biotek) following manufacturer's instructions. For scRNA-seq, FACS-sorted cells were resuspended in PBS and mixed with 10X Gel Beads and reverse transcription reagents in order to form Gel Beads-in-emulsion (GEMs) according to 10X Genomics instructions. For bulk RNAseq, Illumina TruSeq Stranded mRNA Library Preparation Kit with polyA selection. Subsequently, HiSeq 50 Cycle Single-Read Sequencing v4 was performed. For scRNA-seq, 10X Genomics Single Cell 3' Gene Expression Library Prep v3. Subsequently, NovaSeq 2 x 150 bp Sequencing-100M read-pairs per sample was performed.