GSM2977262: 2h_IFNa_MNase-Seq; Homo sapiens; MNase-Seq
For ChIP-seq: Lysates were clarified sonicated nuclei and STAT1, STAT2, and IRF9 was immunoprecipitated with the corresponding antibody.  For Direct selection MNase-seq: Mononucleosomes were isolated following MNase digestion and direct selection via bacterial artificial chromosome hybridization isolation. Libraries were prepared using the 5500 Series SOLiD Systems Fragment Library Preparation protocol using the ABI library preparation kit or NEBNext kit. The fragmented DNA was blunt-ended, size selected for 100-250 bp using Agencourt AMPure XP reagent, and then adding a dA tail for ligation with SOLiD adaptors. The adaptor-ligated DNA was purified using Agencourt AMPure XP reagent and PCR-amplified for 13-15 cycles. The quantity and size was verified using the Bioanalyzer .