GSM4308545: Y6050_KirbyE_Control_single-cells; Mus musculus; RNA-Seq
Cells from two female and one male adult NSPC cultures were harvested with accutase and resuspended and washed in HBSS before making a mixed sample with equal numbers from culture for input to 10x genomics chip as per manufacturer instructions. 10X Genomics 3' RNA-seq library was sequenced using paired-end 150bp approach on an Illumina HiSeq 4000 sequencer. CellRanger v3.0.2 (Zheng et al., 2017) was used to  demultiplex, align, and deduplicate sequencing reads in BCL files. Single-cell data in feature-barcode matrices were then processed using Seurat v3.0.1's default pipeline (Butler et al., 2018) to identify unsupervised cell clusters and generate a t-Distributed Stochastic Neighbor Embedding (t-SNE) plot. Seurat's feature expression analysis was used to visualize genes markers known to be expressed in cell populations displayed in the t-SNE plot.