GSM3588969: B-CTRL, rep3; Rattus norvegicus; RNA-Seq
Total RNA was isolated from amygdala using a Qiagen miRNA easy micro kit (Germantown, MD) which is specialized for isolation of RNA from lipid rich tissue (n=3). Each RNA sample containing 1 µg of RNA was used for poly A selected RNA-seq library preparation using the NEB Ultra-Directional RNA Library Prep Kit for Illumina, Cat# E7420. RNA libraries were prepared for sequencing using standard Illumina protocols