GSM2454610: C2_Ctrl; Chlorocebus aethiops; RNA-Seq
After 72 h post-infection, the cells were harvested and total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) with DNase I digestion (Epicentre, Illumina, San Diego, CA, USA) for 15 minutes at 37℃. The integrity and quality of the total RNA was evaluated using an Agilent 2100 BioAnalyzer (Agilent Technologies) and RNA 6000 Nano Lab Chip Kit (Agilent, CA, USA) with RIN number >7.0. RNA quantity was measured using the NanoDrop 2000 (Thermo Scientific, USA). RNA libraries were prepared for sequencing using standard Illumina protocols