GSM3131864: WT-ribosome profiling input replicate 2; Mus musculus; OTHER
RNA was concentrated by ethanol precipitation and finally dissolved in 10 μl of RNase-free water. The input RNA was first purified by TRIzol and the input mRNA was then separated by Dynabeads mRNA Purification Kit (Invitrogen). The resulting mRNA was concentrated by ethanol precipitation and dissolved in 10μl of RNase-free water. The mRNA was fragmented by RNA fragmentation kit (Ambion). The library of ribosome protected RNA was prepared accoring to NEBNext® Multiplex Small RNA Library Prep Set for Illumina®, while the input library was prepared according to NEBNext® Ultra™ RNA Library Prep Kit for Illumina