GSM2264693: WT O/E_YAD; Saccharomyces cerevisiae; RNA-Seq
RNA was prepared according to the Illumina TruSeq Stranded mRNA Library Prep Kit protocol following addition of 10uL of 1:100 dilution of ERCC92 Mix 1 (Ambion/Life) to each cell pellet as an external spike-in control. Total RNA was DNase treated, and purified with Qiagen RNeasy columns.  Ribosomal RNA was depleted using an Illumina Yeast RiboZero rRNA depletion kit (MRZY1306) RNA fragmentation and priming with Illumina TruSeq Stranded mRNA library synthesis (CAT RS-122-2101). All protocols were performed according to manufacturer’s directions. Single read sequencing was completed on the Illumina HiSeq2500 platform with Version 4 reagents at the Donnelly Sequencing Centre. Single-end reads of 51 bp in length were acquired with a total of 240-250 million reads per lane.