PacBio sequencing of Pantoea agglomerans AR1a
Bacteria were cultured overnight in 5 ml LB broth at 28C with shaking. Cells were pelleted and lysed using standard methods. DNA was extracted from the sample and purified using standard methods. DNA was quantified by PicoGreen and a Nanodrop instrument. For PacBio library preparation, the DNA was sheared to 10-15kb using Covaris g-Tubes with >=7kb size selection on BluePippin using 0.75% cassettes. The library was sequenced on a PacBio PSII (Pacific Bioscience, USA). P6/C4 chemistry and a movie collection length of 6h were used.  Further details of the DNA isolation and sequencing will be presented in a future publication.