16S rDNA Seq of C. teleta and sediment: marine sediment microcosm
V4 region of 16S rRNA gene was amplified using the primer set during the 1st round PCR, then the PCR products were indexed using different combinations of forward and reverse indexing primers during the 2nd round PCR. The pooled and size-selected resulting amplicons were diluted to 8 pM in Illuminas HT1 buffer. Paired-end sequencing reaction was done using a MiSeq platform (Illumina) with V3 chemistry (300-bp read length) at the University of Minnesota Genomics Center (Minneapolis, MN).