HSV1 infected HeLa cells harvested 8 hpi
1 μg of DNase digested total RNA (extracted 8 hpi) was subjected to small RNA library generation using the TruSeq Small RNA Sample Prep Kit according to the manufacturer’s protocol (Illumina). Briefly, after ligation of the RNA 3’- and the RNA 5’adapter, total RNA samples were reverse transcribed to cDNA libraries and subsequently PCR-amplified. The PCR products were run on 6% PAGE gels and bands of adapter-ligated DNA libraries between 145 and 160 basepairs (bp) were excised from the gel and purified. These bands correspond to RNAs of a length of approximately 20 to 35 nucleotides. Subsequently, the libraries were validated by bioanalyzer, pooled and sequenced on a HiSeq 2000 platform (multiplexed 1x51+7).