GSM3728695: Repeat1_Serum_Starved_Day1; Homo sapiens; RNA-Seq
RNA was isolated from the confluent cultures on the appropriate day using Trizol™ (#15596-018, Invitrogen) as per manufacturer's instructions.  The starting volume of Trizol™ was 1.0ml. The RNA pellet was resuspended in 20µl H2O.  RNA concentration was measured using a NanoDrop 100.  RNA quality, as judged by RIN, was measured using an Agilent BioAnalyzer 2100 and RNA Nano Chips.  All the samples had RINs greater than 7.0. Prepare one indexed library from polyA(+)-selected RNA for each sample, load pool on two lanes of HiSeq2000, run as Paired End 100 base reads.