GSM2303801: GC_mock_rep1; Arabidopsis thaliana; RNA-Seq
RNA was isolated using Trizol reagent (Invitrogen) according to the manufacturer’s instructions. RNA quality and quantity were determined using a Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific). Total RNA was purified using the TURBO DNA-free™ Kit (Thermo Fisher Scientific) and first-strand cDNA was synthesized using SuperScript™ II reverse transcriptase (Thermo Fisher Scientific). The cDNA was used for real-time RT-PCR analysis or samples were processed for library preparation. RNA-Seq libraries were prepared starting from 2.5 μg of total RNA using the TruSeq RNA Sample Prep Kit v2 according to the manufacturer’s instructions (Illumina Inc., San Diego, California, USA).