GSM4040410: ONT_REH; Homo sapiens; RNA-Seq
Total RNA was extracted from 20 x 10^6 Reh cells using TRIzol reagent.NucleoTrap Mini kit (Cat# 740655) from Takara was used to enrich for polyA mRNA from 100 ug of total RNA. The library was prepared following manufacturer's protocol using Direct RNA sequencing kit (SQK-RNA002) from ONT. The library was loaded on R9.4 flow cell and sequenced using FLO-MIN106D MinION device for 48 hrs.