GSM2546193: Other, non-PMC cells isolated from 24 hpf embryos, replicate 1; Strongylocentrotus purpuratus; ATAC-seq
Nuclei were extracted using lysis buffer washes and ATAC-seq was performed: 150,000 nuclei were digested with 2.5 ul Tn5 transposase and a Nextera library was created. Nextera library kit used to generate Illumina sequencing library for NextSeq