Truffe du Périgord GA II Simple
Four-month old liquid cultures of Tuber melanosporum Mel 28 grown in 1% malt medium were harvested using liquid nitrogen. RNA extraction was carried out using the RNeasy Plant Mini Kit (Qiagen). Four biological replicates of total RNA preparations were amplified using the SMART PCR cDNA Synthesis Kit (Clontech) according to the manufacturer's instructions and pooled