GSM4041356: Targeted_Bisulfite_GSK343_rep1; Homo sapiens; Bisulfite-Seq
SH-SY5Y cells were washed with 10ml of PBS and genomic DNA were prepared using AccuPrep Genomic DNA Extraction Kit (Bioneer, K-0332). Targeted Bisulfite-Seq library were prepared using the SureSelectXT Methyl-Seq Target Enrichment System for Illumina Multiplexed Sequencing (Agilent Technologies). Briefly, 3μg of genomic DNA per sample was fragmented to median size of 150bp, repaired, and ligated with adapters. The prepared gDNA library was hybridized using SureSelectXT methyl-Seq capture reagent, followed by bisulfite conversion using EZ DNA Methylation-Gold kit (ZymoResearch, Cat #D5005). After desulphonation, the sequence-modified, target-enriched library were amplified and uniquely indexed by PCR. The indexed libraries were pooled and multiplexed into 4 separate lanes using 100 bp paired-end sequencing (Illumina HiSeq2500).