GSM4475256: cAMP_under_C/EBPb-knockdown; Homo sapiens; ChIP-Seq
See library construction protocol Cultured cells were cross-linked by adding formaldehyde to the medium at a final concentration of 1% and incubated for 10 min at 37 °C.  Cross-linking was terminated by addition of glycine (0.125 M, final concentration).  ChIP was performed with H3K27ac antibody.  An unimmunoprecipitated control and immunoprecipitated DNA were used to prepare the ChIP-seq library with NEBNext DNA sample prep master mix set 1 (New England BioLabs, Ipswich, MA) and DNA sample prep kit, oligo only (Illumina) according to the manufacturer's instructions.