GSM4405430: CC_SMC3_3; Mus musculus; RNA-Seq
Cells were sorted into Trizol reagent (Invitrogen) and RNA was extracted following the manufacturer instructions. RNA quality was evaluated using Agilent Technologies 2100 Bioanalyzer and samples that passed the quality control (RNA Integrity Number ≥8) were subjected to library preparation Library preparation, sequencing and post-processing of the raw data was performed at the Epigenomics Core at Weill Cornell Medicine. Illumina TruSeq RNA sample kits were used for libarary preparation, according to the manufacturer.