RNA-seq of vibrio natriegens: log phase
The total RNAs of the bacterial cells were extracted with the Trizol (Sigma-Aldrich, USA). RNA quality was checked through agarose gel electrophoresis. The RNAs were then quantifed with a spectrophotometer. The RNA samples were subsequently subjected to cDNA library construction and deep sequencing with Illumina HiSeq X Ten platform (San Diego, CA, United States) with 150PEmode.