GSM4155847: H3K36me3_4h_r1; Saccharomyces cerevisiae; ChIP-Seq
Cells were fixed with 1% formaldehyde for 15 minutes at RT with mild shaking. Crosslinking was halted with 125 mM Glycine for 5 minutes. Cells were washed with PBS and the pellet was flash frozen in liquid nitrogen. Cells were lysed by bead beating, the chromatin was sheared by sonication, and protein-DNA fragments of interest were isolated with a specific antibody and magnetic protein A Dynabeads. Libraries were prepared as instructed by the ThruPLEX DNA-seq Kit (R400427, Takara). 15 ng of starting material was used with 7 rounds of PCR. AMPure XP beads (A63881, Beckman Coulter) were used to select fragments between 200-500 bp.