GSM4040382: Eye disc rep 2; Drosophila melanogaster; RNA-Seq
RNA-seq was performed on three biological replicates, each consisting of 30 third instar larval eye or antennal discs. Eye-antennal discs were dissected in 1X PBS and separated from the mouth hooks. Eye discs and antennal discs were then manually separated and placed directly into 300 mL of Trizol. RNA was purified using a Zymo Direct-zol RNA MicroPrep kit (catalog number R2062). mRNA libraries were prepared using an Illumina TruSeq Stranded mRNA LT Sample Prep Kit (catalog number RS-122-2101).