Normal
Genomic DNA from all uterine fibroids and corresponding normal myometrium were extracted from freshly frozen tissue using DNeasy Blood and Tissue kit (Qiagen) according to manufactures recommendation. Samples were submitted to Hudson Alpha (Huntsville AL) for 2 x 100 sequencing on an Illumina HiSeq 2500. In total, 8 fibroids and matched normal tissue pairs were sequenced. Four of the eight sample pairs had an additional fibroid sample sequenced. Exome capture was performed using the NimbleGen SeqCap EZ Exome v3 kit and sequenced to a depth of approximately 45x across two flow cells.