GSM2720312: BMS-21 6h rep 1; Rattus norvegicus; RNA-Seq
Following incubation with treatments, cells were washed (PBS) and, following RNA isolation Kit 2 (Bioline) manufacturers instructions, RNA was isolated. RNA quality was assessed via examination of nucleic acid concentration and purity using a Nanodrop spectrophotometer. RNA sample quality was further analysed using a bioanalyser and had RIN > 8. mRNA libraries were constructed from total RNA using TruSeq RNA Library Prep Kit v2 for sequencing using standard Illumina protocols