WGS sequencing of Sphenodon punctatus
High-molecular-weight genomic DNA was extracted from ~100 ul of snap-frozen blood using proteinase K digestion with Phenol-Chloroform extraction, and performed at Dovetail Genomics (Scotts Valley, CA). A PacBio SMRTbell library was prepared using the SMRTBell Express Template Preparation Kit (Pacific Biosciences) following the PacBio 15-kb template preparation protocol, with a minimum size cutoff of 5kb size selection used in the size selection stage with the Sage Science BluePippin system. The prepared library was run on the PacBio Sequel platform (Pacific Biosciences) using version 2.1 chemistry. The Single Molecule Real-Time (SMRT) Cells was sequenced on a SMRT cell with 360min movie lengths.