GSM2264504: sample_s2; Mus musculus; RNA-Seq
Total RNA was extracted using the Nucleospin RNA XS kit (Clontech, Mountain View, CA). The TruSeq RNA sample preparation kit (Illumina, San Diego, CA) was used to prepare cDNA libraries from RNA samples. Samples were polyA selected to isolate mRNA, the mRNA was cleaved, reverse transcribed, end-repaired and enriched as per the manufacturer's protocol.