dds85 whole genome sequencing
Genomic DNA was isolated from individual saturated overnight cultures using the MasterPure Yeast DNA Purification Kit (Epicentre) according to the manufacturerŐs instructions. Purified genomic DNA was treated with RNase A/T1 Cocktail (Ambion), phenol extracted, precipitated with ethanol, and quantified with the Qubit dsDNA HS Assay Kit (Invitrogen). For each strain, 1 ng of genomic DNA was used as input into the Nextera XT DNA Library Preparation Kit (Illumina) and genomic DNA libraries were prepared according to the manufacturerŐs instructions using the Nextera XT Index Kit for barcoding during PCR. Genomic DNA libraries from each strain were quantified with the Qubit dsDNA HS Assay Kit (Invitrogen), and pooled into a single tube in equal proportions. Pooled DNA was purified using the DNA Clean & Concentrator-5 Kit (Zymo Research), and 200Đ350-bp fragments were isolated using a BluePippin Gel Cassette (2% agarose dye-free with internal standards). The size-selected library was sequenced on a single lane of a HiSeq 4000 sequencer using 50-nucleotide single-end reads.