GSM2545787: ChIP-Seq H3K4me2_2i; Mus musculus; ChIP-Seq
For ChIP-seq, cells were fixed with 2mM disuccinimidyl glutarate (DSG) for 45 mins and 1% formaldehyde for 10mins, and followed by glycine for 5 mins. Chromatin DNA was sheared to 200–500 bp average in size by sonication and chromatin was immunoprecipitated with antibodies. Protein G dynabeads (Invitrogen) were added and incubated overnight at 4°C. After washing and elution, the protein–DNA complex was reverse-crosslinked by heating at 65°C, and immunoprecipitated DNA was purified by using QIAquick Spin column. the libraries were constructed following Illumina's Chip-Seq Sample prep kit.