GSM4473370: Epicardial Cells from H9-ESCs-R1; Homo sapiens; RNA-Seq
RNA was extracted from cell lysates using Zymo's Direct-zol RNA Miniprep Kit (Zymo cat. 2050) Libraries were prepared using Illumina mRNA library Prep Kit (Illumina cat.  RS-122-2001) RNA was reverse transcribed to cDNA. cDNA libraries were prepared using 500ng of total RNA using the TruSeq mRNA library prep kit. mRNA-seq and data analysis cDNA libraries were sequenced on the Illumina Nextseq platform to obtain 80-bp single-end reads. 