GSM2546189: Whole 28 hpf embryos, replicate 2; Strongylocentrotus purpuratus; DNase-Hypersensitivity
Nuclei were extracted using lysis buffer washes and DNase-seq was performed: 10 million nuclei per replicate were digested with 0-400 units DNase 1 for 3 mins at 37 degrees. Digested fragements < 500 bp were purified. Truseq library kit used to generate Illumina HiSeq sequencing library by USC Epigenome Center