GSM3066023: HEL-iMKL1 Dox treated cells rep1; Homo sapiens; RNA-Seq
RNA was extracted with Trizol. The RNA layer was then purified using the RNeasy Mini Kit columns from Qiagen (Hilden, Germany). RNA was eluted in a total of 20µl TE buffer, and quantified using a standard Nanodrop device. Single-end RNA libraries were prepared for sequencing using standard Illumina protocols at the Yale Stem Cell Center Genomics Core