GSM2720252: 23_M5CreMinus_MC_6mo; Mus musculus; RNA-Seq
CD45 cells excluding mast cells (CD117 high, FceR1 high) were sorted from digested skin. Total RNA was solated using the Qiagen RNeasy Micro Kit+ SMARTer Ultra Low Input RNA for Illumina Sequencing Kit - HV from Clontech was used to reverse transcribe the RNA and amplify full-length cDNA according to the user manual. It was followed by a library prep using NEBNext Ultra DNA Library Prep Kit for Illumina (NEB).