GSM3067544: DKO_WGBS; Mus musculus; Bisulfite-Seq
1 μg of genomic DNA was isolated from mESCs and fragmented using a Covaris sonication system (Covaris S2). Following DNA fragmentation, libraries were constructed using the Illumina TruSeq DNA sample preparation kit. After ligation, libraries were treated with bisulfite using the EpiTect Bisulfite kit (Qiagen, 59104). Ligation efficiency was tested by PCR using TruSeq primers and Pfu Turbo Cx Hot-Start DNA polymerase (Stratagene, 600632). After determining the optimal number of PCR cycles for each sample, a large-scale PCR reaction (100 μl) was performed. PCR products were sequenced with Illumina HiSeq sequencing systems.