GSM2454874: SW480_72h_DMSO_3; Homo sapiens; RNA-Seq
medium was aspirated and cells washed with PBS. Total RNA was isolated using the RNeasy kit (Qiagen) and RNA amount was measured using Qubit 2.0 Fluorometric Quantitation system (Life Technologies). RNA integrity number (RIN) was determined using Experion Automated Electrophoresis System (Bio-Rad). RNA-seq libraries were prepared with TruSeq Stranded mRNA LT sample preparation kit (Illumina) using Sciclone and Zephyr liquid handling robotics (PerkinElmer). Library amount was quantified using Qubit 2.0 Fluorometric Quantitation system (Life Technologies) and the size distribution was assessed using Experion Automated Electrophoresis System (Bio-Rad). For sequencing libraries were pooled, diluted and sequenced on Illumina HiSeq 2000 using 50 bp single-read chemistry.