Psoroptes ovis var. cuniculi RNA-seq - nymphs and adults
A second generation sequencing platform (RNA-Seq) was applied. Total RNA was extracted with Trizol reagent (Invitrogen) from nymphs and adults of P. ovis var. cuniculi in accordance with the manufacturer’s recommendations. The RIN value of nymphs and adults samples was >7.0. Sequencing libraries were obtained by applying Illumina TruSeq™ RNA Sample Preparation Kits (San Diego, USA) according to the manufacturer’s instructions, and index codes were added to attribute sequences to each sample. Finally, PCR products were purified on the AMPure XP system and the qualities of the sequencing libraries were assessed on the Agilent Bioanalyzer 2100 system. The library preparations were sequenced on an Illumina HiSeq 2000 platform.