GSM3589423: Gold_onlyPL_group1; Canis lupus familiaris; RNA-Seq
Following surgery, placentae were separated from uteri, rinsed with phosphate-buffered saline (PBS) and immersed in RNAlater® for 24h at +4°C. After 24h, tissue material was stored at -80°C until RNA isolation. RNA was isolated using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit v2 was used with 100-1000ng/per sample of total RNA for the construction of sequencing libraries.