Early scleroderma patient sample RNA seq
3 mm punch biopsies were obtained from lesional forearm skin from 8 Nilotinib clinical trial SSc patients ( with disease duration < 3.0 year classified as early stage . Total RNA extraction was performed using the Qiagen RNeasy Fibrous Tissue Mini Kit. RNA-seq libraries were generated from 100 ng total RNA prepared using the Illumina TruSeq Stranded Total RNA Library Prep Kit with Ribo-Gold rRNA depletion. Samples were then multiplexed and sequenced on anNextSeq 500 sequencer, producing an average of 80100 million 75-bp paired-end reads per sample.