GSM4155864: input_4h_r3; Saccharomyces cerevisiae; ChIP-Seq
Cells were fixed with 1% formaldehyde for 15 minutes at RT with mild shaking. Crosslinking was halted with 125 mM Glycine for 5 minutes. Cells were washed with PBS and the pellet was flash frozen in liquid nitrogen. Cells were lysed by bead beating, the chromatin was sheared by sonication. Libraries were prepared as instructed by the ThruPLEX DNA-seq Kit (R400427, Takara). 15 ng of starting material was used with 7 rounds of PCR. AMPure XP beads (A63881, Beckman Coulter) were used to select fragments between 200-500 bp.