RNA-Seq of Microphallus sp.: metacercariae collected from Potamopyrgus estuarinus
We isolated Microphallus metcercariae from a single Potamopyrgus estuarinus individual collected from the Ashley River Estuary, South Island, New Zealand. RNA was extracted from metacercariae using TRIzol. RNA quantity and quality were assessed with a Bio-Rad Experion Automated Electrophoresis Station, following manufacturer protocol, requiring an RQI >/= 8 and a minmum of 2ug of total RNA. We generated cDNA libraries following the Illumina TruSeq LS protocol, and then performed 2x100bp paired-end RNA sequencing on an Illumina HiSeq 2000.