GSM3591418: Control (Pol IV rdr2 mutant); synthetic construct; ncRNA-Seq
RNA transcripts were precipitated with isopropanol after in vitro transcription RNA transcripts were prepared according to Illumina's instructions accompanying the TruSeq small RNA Prep Kit. Briefly, for precursors produced by Pol IV and RDR2 and siRNAs produced by DCL3 dicing of the precursors, 80 cycles of paired-end sequencing was performed. A replicate precursor sample produced by Pol IV-RDR2 and a control sample produced by Pol IV extracted from Pol IV rdr2 mutant background were subjected to 73 cycles of single-end sequencing.