GSM3417884: G3_T-DM1; Mus musculus; RNA-Seq
Tumors were harvested 10 days after treatment (d10) and mechanically digested with accutase (PAA), collagenase IV (Worthington), hyaluronidase (Sigma), and deoxy-ribonuclease type IV (Sigma). Single-cell suspensions were then prepared and CD45-positive cells were enriched using the EasySep Mouse PE Positive Selection Kit (STEMCELL Technologies). RNA of CD45+ cells was extracted and RNA-seq libraries were prepared using the Truseq stranded mRNA Library Prep Kit from Illumina with 200 ng input material. Samples were run on an Illumina NextSeq SR81 using the NextSeq 500/550 High Output v2 kit (75 cycles).