GSM3669538: S19_down_mem_3; Homo sapiens; OTHER
The cell lysate was clarified by centrifugation, treated by RNase I, cross-linked RNA-uS19 complexes were isolated by anti-FLAG-M2 antibodies with subsequent purification by PAGE-SDS electrophoresis followed by transfer onto nitrocellulose membrane. RNA fragments were recovered by proteinase K treatment followed by phenol-chloroform extraction. DNA libraries were prepared according to (Kargapolova Y, 2017, doi: 10.1093/nar/gkx152)