RNA-Seq of Escherichia coli BL21(DE3)
The RNA from E. coli BL21 (DE3) induced by IPTG and non-induced by IPTG were extracted by RNA extraction kit (Biomiga), respectively. 2% agarose gel electrophoresis was used to analyze the integrity of RNA. Nanodrop was used to detect the purity of RNA, and Qubit was used to quantify concentration of RNA. The transcriptome sequencing was completed by Beijing allwegene Bioinformatics Technology Co., Ltd (Beijing, China)