AB 5500xl Genetic Analyzer paired end sequencing; RNAseq of PBMC from HIDS patients
RNAseq of PBMC from HIDS patients
Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation from blood samples of VPER and VRIS using Ficoll-Paque™. PBMC  cells (106 cells) were seeded in 24-well plates in RPMI1640 supplemented with fetal calf serum (FCS), penicillin, streptomycin, amphotericin B (all from Invitrogen) and stimulated with LPS (1 µg/mL;  from Salmonella Abortus equi ; Sigma, Saint-Quentin-Fallavier, France) for 3 h or 6 h at 37 °C in a humidified atmosphere containing 5% CO2. The control condition without LPS stimulation is named mi. Description of the sample naming: lps: stimualtion with lps - mi: no stimulation - 3h/6h: lps stimulation for 3 or 6 hours - _1 or _2: replicate 1 or 2 - VPER: subject VPER - VRIS: subject VRIS Trizol SOLiD Total RNA-Seq Kit