GSM2263154: RRBS_Heart_1wk_WT_rep7; Mus musculus; Bisulfite-Seq
DNA was isolated from mouse hepatocytes or from snap-frozen mouse tissues and incubated in lysis buffer (25 mM Tris-HCl (pH  8), 2 mM EDTA, 0.2% SDS, 200 mM NaCl) supplemented with 300 μg/ml proteinase K (Roche) followed by phenol:chloroform extraction and ethanol precipitation. RRBS libraries were prepared as described (Boyle et al., 2012) and run on Hiseq 2500 (Illumina).