GSM3462375: Ciz1NullAdapted_S12; Mus musculus; RNA-Seq
RNA was extracted with TRIzol (Ambion 15596-026) following manufacturer's instructions, then treated with DNase (Roche 04716728001), before quality analysis by agarose gel, NanoDrop spectrophotometer and Agilent 2100 Bioanalyzer. Libraries were prepared with NEBNext® UltraTM RNA library Prep Kit for Illumina®, and enriched for mRNA using NEBNext Poly(A) mRNA Magnetic Isolation Module, which is optimized for production of libraries with 250-400bp inserts.