GSM3728292: HCI-005_ER_DHT; Homo sapiens; ChIP-Seq
Tumors were cut into 30µm slices on a cryostat and fixed with formaldehyde for 10m prior to quenching. Chromatin was prepared and subsequently sheared by the Diagenode BioRuptor Pico for 5.5 cycles (30 sec on, 30 sec off) on ice. Sheared chromatin was immunoprecipitated with ERα antibody overnight, after coupling to Protein A Dynabeads (10002D). DNA was purified using phenol:chloroform:isoamyl alcohol extraction. Libraries were prepared according to manufacturer's instructions accompanying the Qiagen Ultralow Input Library Kit (Part# 180495). ChIP-Seq; Illumina NextSeq500 (High Output v2) single-end 75 bp reads.