Off-target analysis after treatment with HE in mice with latent HSV
PCR products were diluted to 1ng/ul and quarter-volume NexteraXT reactions were performed following the manufacturerŐs protocol (Illumina) with a sample volume of 1.25ul.  Libraries were amplified and barcoded using 12 cycles of PCR with the NexteraXT Index Kit (Illumina) and samples were pooled and quantitated by Bioanalyzer (Agilent) such that approximately 200,000 80 bp reads were achieved per sample on a MiSeq sequencer (Illumina).