GSM3589693: HetU rep1 (control); Mus musculus; RNA-Seq
Total RNA was extracted from placenta using RNeasy Kits (Qiagen – 74134). Quantity and quality were verified using the RNA 6000 Nano Kit (Agilent – 5067-1511) and an Agilent 2100 Bioanalyzer. Only RNA samples with RNA integrity numbers (RIN) >9.0 were used. Libraries were prepared using TruSeq (Illumina), the barcoded libraries were combined and submitted for sequencing (50bp single ended on a HiSeq2500) through the Wellcome Trust-MRC Institute of Metabolic Science Genomics Core Facility.