GSM4105025: 2hpa_ATAC-seq rep2; Xenopus tropicalis; ATAC-seq
ATAC-Seq libraries were prepared from 20 tadpole tail tips in biological duplicate using independent clutches. Tissue was homogenized by incubating in liberase (0.35mg/mL) for 10 minutes at room temperature.  Cells were gently spun at 2100 rpm for 2min, and the resulting cell pellet was then incubated in a transposition mix DNA was fractionated via transposition with a modified Tn5 transposase (Nextera DNA Sample Prep, Illumina), where sequencing adapters are also integrated to the fragment ends.  Libraries were PCR amplified with cycle numbers determined by a side qPCR reaction.  Following purification, libraries were quality checked for expected banding patterns on a 5% Bio-Rad Mini-Protean TBE Precast gel.  ATAC-Seq libraries were paired end sequenced on HiSeq and NextSeq platforms.