RAN  Seq jujube leaf infected by phytoplasma
The sequencing libraries were constructed using the NEBNext? Ultra ? RNA Library Prep Kit (NEB, USA) according to protocol. mRNA was identified from total RNA using Oligo (dT) attached magnetic beads, and then short fragments were obtained. First-strand cDNA was transcribled using M-MuLV Reverse Transcriptase (NEB, USA). Second-strand cDNA synthesis was obtained using 10 mM dNTP Mix, 5 U/?l RNase H and10 U/?l DNA polymerase I. The obtained cDNA were then purified, A-tailed, and ligated to sequencing adapters. Fragments were deemed suitable templates for PCR amplification. The RNA-seq library was sequenced on an Illumina Hiseq? 2500 platform (Illumina) and 150-bp reads were produced. Data analysis was performed by the Illumina instrument software.