GSM2107950: Rrp6-HTP_1; Saccharomyces cerevisiae; OTHER
UV stabilized protein-RNA interactions were purified under denaturing conditions and RNAs associated with the protein of interest were partially truncated. Sequencing 3' adapter and 5' adapter were ligated while Rpb1-RNA complexes were bound to Ni-NTA agarose, RNA library was reverse transcribed and PCR amplified.