Vitamin A sufficient Mouse 6
Cecal contents were used for a DNA extraction with the E.Z.N.A kit from omega. Amplification was preformed with PCR. The PCR conditions are as followed: 3 min incubation at 94 _ followed by 25 cycles of 94 _, 20 s; 55 _, 45 s; 72 _, 1 min and final one cycle of 72 _ for 8 min.