Hi-C of vibrio natriegens: log phase
Each sample were collected and used for a Hi-C pipeline as described in Lieberman-Aiden E at Science (PMID: 19815776). Cultured strains were collected by centrifugation and washed by TE (10 mM Tris-HCl (pH 8.0), 1 mM EDTA) and crosslinked with 3% final concentration fresh formaldehyde for 10 minutes at room temperature (RT). Fixed cells were frozen in liquid nitrogen and stored at -80 until use.