GSM4406980: Vehicle_Rep2; Mus musculus; RNA-Seq
Tibalis anterior muscles were removed, digested using collagenase II and dispase, and muscle stem cells (Ter119-CD31-CD45-Sca1-CD11b-CXCR4+B1int+) were sorted on a BD FACS Aria III into tryzol. Qiagen mRNeasy Micro kit was used to extract RNA according to manufacturer's instructions. Integrity was determined using an Aligent Bioanalyzer, and samples with a RIN value below 0.9 were discarded. Takera Bio SMARTseq v4 cDNA synthesis kit was used with 1ng of RNA according to manufacturer's instructions. Libraries were prepared using the Nextera XT DNA Library Preparation Kit according to manufacturer's instructions.