GSM2869296: Eo [CAGRF9188-1422]; Mus musculus; RNA-Seq
FACS sorted cell pellets were lysed and RNA prepared on same day using QIAGEN RNeasy plus mini or micro kit with B-mercaptoethanol added to inhibit RNAses. RNA was submitted to the Australian Genome Research Facility (AGRF) for high throughput mRNA sequencing. Messenger RNA libraries were synthesized using Illumina's TruSeq Stranded mRNA sample preparation protocol, and 100 bp single or paired end reads were generated by high-throughput sequencing with an Illumina HiSeq 2500 (Illumina). 2-5 independent RNA samples (biological replicates) per cell type were sequenced.