GSM3667186: Irf5-/- BMDMs, infected with Rickettsia parkeri, with IFN-I; Mus musculus; RNA-Seq
At 12 hpi, cells were lysed and RNA was purified using an RNeasy purification kit (Qiagen). Dnase-treatment was performed on column according to the manufacturer's protocol. RNA quality was assessed using an Agilent 2100 Bioanalyzer, and all samples had RIN values above 8.0. Transcripts were selected using polyA selection (using Dynabeads mRNA Purification Kit, Invitrogen) and enzymatically fragmented as part of the Apollo library prep kits (Wafergen PrepX RNA library prep for Illumina). Libraries were constructed by using Apollo 324 (IntegenX Inc.), PCR amplified, and multiplexed at the Functional Genomics Lab at the University of California, Berkeley (http://qb3.berkeley.edu/qb3/fgl/). The resulting libraries were sequenced at the Vincent J. Coates Genomics Sequencing Facility at the University of California, Berkeley using single-end reads, 50 base length, with a Hiseq 2000 Illumina platform.