GSM3591611: P6_TNFa; Homo sapiens; OTHER
Cells were fixated in 1% formaldehyde to be used for promoter capture Hi-C as previously described (Creyghton, M.P., et al., Proc Natl Acad Sci U S A, 2010; Rada-Iglesias, A., et al., Nature, 2011.) Promoter capture was performed by using 37,608 biotin-labeled RNA baits (each 120 nucleotides) covering 21,841 human promoters (approximately two baits per promoter targeting each end of a HindIII fragment were designed). Baits were synthesized by Agilent Technologies and hybridization was performed using the Sure Select Target Enrichment kit ILM (Agilent Technologies) and SureSelect XT library prep kit ILM (Agilent Technologies) according to manufacturer's instructions. DNA libraries were sequenced on a HiSeq2000 by 75‐bp paired‐end sequencing at the National High‐Throughput Sequencing Centre (University of Copenhagen, Denmark).