GSM4594677: R122Pfs-3; Danio rerio; RNA-Seq
Total RNA was extracted from individual brains using the mirVana miRNA isolation Kit (Ambion, Life Technologies, Thermo Fisher Scientific, Waltham, MA, USA).  DNase treatment was performed on RNA to remove remaining genomic DNA using the DNA-free Kit (Ambion, Life Technologies, Thermo Fisher Scientific, Waltham, MA, USA). PolyA amplification was performed for the construction of sequencing libraries. Demultiplexed libraries were provided by the sequencing centre at the South Australian Health and Medical Research Institute (SAHMRI) as 75bp single-end reads.