GSM3132626: SP4_RNA-seq_rep3; Oryza sativa; RNA-Seq
Tn5 Transposase treatment (Using the Illumina Nextera kit) was performed on the intact nuclei isolated by Plant Nuclei Isolation/Extraction Kit (CELLYTPN1, sigma) from each sample, followed by isolation of fragmented genomic DNA using the Qiagen PCR purification kit. Purified transposase-fragmented DNAs were converted to sequencing libraries through a limited number of PCR cycles using indexed primers. Total RNA of WXS was isolated using TRIzol reagent (Invitrogen) according to the manufacturer's instructions. Chromatin from panicle of WXS was immunoprecipitated with antibodies against H3K4me2 and H3K9ac. The eluted ChIP DNA was used to generate Illumina sequencing libraries following the manufacturer's protocol. The genomic fragments of Tn5, RNA libraries and ChIP-ed DNA were separately prepared for sequencing using standard Illumina protocols