Amplicon sequencing for the mutation in iscR of Escherichia population isolated at day 15  from old mouse A3
The sequencing was performed at the genomics facility at Instituto Gulbenkian de Ciencia (Portugal). Specific primers for iscR (iscR_Fw and iscR_Rv) were used for library construction. Briefly, a sample of mice feces was plated on LB agar and allowed to grow till ~1000 isolated colonies were visible. These colonies were then pooled and DNA was extracted. A PCR was then performed using the primers iscR_Fw and iscR_Rv and the product was used for amplicon sequencing.