GSM2303940: BLD101_WT; Lactococcus lactis subsp. cremoris; RNA-Seq
Total RNA was isolated using the RNeasy Mini Kit (Qiagen). RNA was treated with DNase, purified using the RNA Cleanup protocol from the RNeasy Mini Kit (Qiagen), and stored at −80°C. The integrity of the RNA was analyzed using an Agilent 2100 Bioanalyzer (Agilent Technologies). Removal of rRNA was performed using the RiboMinus Bacteria Module (Invitrogen). The transcriptome library for sequencing was constructed according to the NEXTflexRapid Directional RNA-Seq library protocol (Bioo Scientific). The prepared library (insert size of 130-580 bp) was validated for quality by running an aliquot on a High Sensitivity Bioanalyzer Chip (Agilent).