GSM3669258: TFAM overexpression, ATF4 RNAi rep 1; Drosophila melanogaster; RNA-Seq
For each replicate, the complete CNS from 20 wandering third instar larvae were dissected in PBS and then transferred into 100ul of lysis buffer (with β-mercaptoethanol) from the Absolutely RNA Microprep kit (Stratagene) and vortexed for 5 seconds. Total RNA was then prepared using this kit. For each genotype RNA samples were prepared in quadruplicate and stored at -80 degrees celsius. RNA samples were couriered on dry ice to the Glasgow Polyomics facility (University of Glasgow), where polyA cDNA libraries were constructed using the TruSeq Stranded mRNA Kit (Illumina).