454 sequencing of Rhizophora mangle American red mangrove transcript fragment library
cDNA were nebulized and size selected for an average size of 400-500bp. 454FLX specific adapters, were ligated to the cDNA ends after the end polishing reaction. The adapter ligated DNA was then mobilized to the library preparation beads to capture ssDNAs that were used for clonal amplification in emPCR reactions. Following emPCR the amplified beads were loaded into the 70x75 PicoTiterPlate (PTP), followed by packing beads and enzyme beads.  The PTP was then placed onto the GSFLX Genome Sequencer. Sequencing was done at the University of Illinois W.M. Keck Center.