Transcriptome of female Diversinervus elegans
Total RNA was extracted from female adults using Trizol reagent (Invitrogen). Its quantity and quality were assessed by the Qubit 2.0 Flurometer (Life Technologies) and Agilent 2100 Bioanalyzer (Agilent), respectively. Library preparation was performed using the NEBNext Ultra RNA Library Prep Kit for Illumina. The first-strand cDNA was synthesized using random hexamer primer, DNA Polymerase I and RNase H. The prepared library was sequenced on an Illumina Hiseq platform from paired-ends.