GSM4476659: WT_d10_B1; Macaca fascicularis; RNA-Seq
Blastocysts at Day 10, Day 12 and Day 14 were washed with PBS and treated with TrypLE Express Enzyme (Thermo Fisher Scientific) for 30 minutes at 37℃. Then dissociate single cells by mouth pipetting until no bulky cell clumps could be observed. Single cells were transferred into a RNase-free, low-cell-adhesion 1.5 mL tube and centrifuged at 300 ℊ for 5 minutes. Carefully remove the supernatant and resuspended the cell pellet with 40 µL of 2% BSA in PBS. Within 30 minutes after dissociation, cells were loaded into the 10x Genomics Chromium system. Libraries for sequencing were prepared using the Chromium Single Cell 3ʹ Reagent Kits v3 according to the manufacturer's instructions.