GSM2290760: TRPC2_1; Danio rerio; RNA-Seq
Total cellular RNA was extracted using Qiagen Rneasy micro kit. T7-oligodT primers were used for reverse transcription of mRNA during cDNA synthesis. RNA amplification was performed using T7 Megascript kit through one (for OMP neurons) two rounds (for TRPC2 neurons). Illumina TruSeq v2.0 kit was used as per manufacturer's protocol.