GSM2545689: prp8-N1869D_TechRep_1; Saccharomyces cerevisiae; RNA-Seq
Cells were harvested by filtration, flash frozen in liquid Nitrogen and RNA was extracted using hot acid-phenol, chloroform extraction. 5ug total RNA was used as input for Illumina Ribo-Zero Gold kit (Yeast) followed by treatment with RNaseR (Epicentre). RNA libraries were prepared for sequencing using altered double strand cDNA synthesis conditions followed by standard Illumina TruSeq RNA-seq protocol with modified cleanup strategy between steps.