Ad2 infected HeLa cells harvested 27 hpi
1 μg of DNase digested total RNA (extracted 27 hpi) was subjected to small RNA library generation using the TruSeq Small RNA Sample Prep Kit according to the manufacturer’s protocol (Illumina). Briefly, after ligation of the RNA 3’- and the RNA 5’adapter, total RNA samples were reverse transcribed to cDNA libraries and subsequently PCR-amplified. The PCR products were run on 6% PAGE gels and bands of adapter-ligated DNA libraries between 145 and 160 basepairs (bp) were excised from the gel and purified. These bands correspond to RNAs of a length of approximately 20 to 35 nucleotides. Subsequently, the libraries were validated by bioanalyzer, pooled and sequenced on a HiSeq 2000 platform (multiplexed 1x51+7).