GSM3462589: 1072E-ECTO; Homo sapiens; RNA-Seq
Tissue RNA was extracted using total RNAase Fibrous Tissue Mini Kit (Qiagen, Valencia, CA). The concentration and purity of the extracted RNA were assessed by the Agilent Bioanalyzer (Agilent, Santa Clara, CA). RNA was labeled and sequenced at the RU Genomics center by using Illumina TruSeq technology (75bp, >30M coverage). 100 ng of total RNA was used to generate RNA-Seq libraries using Illumina TruSeq stranded mRNA LT kit (Cat# RS-122-2101). 