GSM3901548: 1μMGSK343treatment_K562_GSK343_rep2_techRep2_RNASeq; Homo sapiens; RNA-Seq
Total RNA was extracted from cells using RNeasy Mini Kit (Qiagen), with on-column DNase I treatment (Qiagen). The quality of the RNA extracted was checked using Agilent RNA 6000 Nano Kit (Agilent), and quantitated using Nanodrop ND1000 (Thermo Scientific). 850ng of RNA was used for the construction of cDNA library for Illumina HiSeq 2500 High Output v4 NGS sequencing using TruSeq Stranded Total RNA LT (w/ Ribo- Zero Gold) Set A (Illumina) as per protocol.