GSM4474482: 4R128-L2-T1_Day3_LacZ; Homo sapiens; RNA-Seq
Reprogramming cells underwent MEF depletion using magnetic bead separation (Miltenyi Biotec). Then total RNA was extracted from remaining cells using Direct-zol RNA Miniprep Kit (Zymo). RNA libraries were prepared for sequencing using standard Illumina protocols. Biological replicates of each sample sequenced in separate flow cell, except for Day -1.