GSM2978130: WTN_rep1; Mus musculus; RNA-Seq
Total RNA was extracted from ~30 mg of fresh frozen lung tissue by using the RNeasy Mini plus Kit (Qiagen, Cat # 74134). RNA quality was analyzed with an Agilent 2100 Bioanalyzer (Agilent Technologies). Enrichment of mRNA was performed by NEBNext® Poly(A) mRNA Magnetic Isolation Module (Cat # E7490S) from 1 ug of mRNA. cDNA libraries were constructed using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (Cat # E7530S).