water from the storage pond
The V4 hypervariable region of the 16S rRNA gene was amplified using the 515F and 806R primers with sample-specific barcodes. PCR was performed using 30-l reaction volumes with 15 l of Phusion High-Fidelity PCR Master Mix (New England Biolabs, USA), 0.2 M of each primer, and approximately 10 ng of template DNA.