GSM4475671: MFB23; Drosophila melanogaster; RNA-Seq
Single cells were sorted via fluorescent-activated cell sorting. Each cell was sorted in a separate well of a 96 well plate  filled with freshly made lysis buffer, prepared according to Takara standard protocol. The contact with the lysis buffer lysed the cells, freeing their content, mRNA included. As soon as collection was completed (15-20 minute/genotype), samples were frozen in dry ice and stored until used at -80°C. Sequencing libraries were prepared using the Nextera XT kit (Illumina #FC-131-1096) and mixed into 24 pools (12 samples per pool).After purification using the Agencourt AMPure XT beads (Beckman Coulter #A63881), the sample quality was checked with both the Qubit 3 Fluorometer and the High Sensitivity D1000 ScreenTape assay (Agilent Technologies #5067-5584). The libraries were equimolarly pooled, and the final concentration was estimated by qPCR. Sequencing of 75 bp paired-end reads was performed with the Illumina Nextseq 500 sequencer. Nextera XT