WGS of Strix occidentalis caurina: adult female blood
We isolated DNA using a Gentra Puregene Kit (Qiagen) and sent 41.3 µg to GENEWIZ (www.genewiz.com). We requested a barcoded mate-pair library with an insert size of 11 kb constructed using the Nextera Mate Pair Sample Preparation Kit (Illumina). GENEWIZ followed the procedure detailed in the Nextera Mate Pair Sample Preparation Guide (Illumina, Part # 15035209 Rev. C, January 2013). Traces obtained using a 2100 BioAnalyzer (Agilent Technologies) showed the center of the distribution of the sheared fragments that went into the circularization step as 10.7 kb. GENEWIZ pooled the library with 2 others equimolarly and we consequently obtained approximately 1/3 of one lane of 100 bp paired-end data sequenced on a HiSeq 2000 (Illumina).