Mycobacterium sp. VKM Ac-1817D grown on a medum with phytosterol and with MCD, biological replicate 1
Total RNA was isolated using Qiagen RNeasy mini kit. The resulting mixture was treated with DNase I. Ribosomal RNA was depleted using with Ribo-Zero rRNA Removal Kit (Bacteria) (Epicentre, USA). Sample preparation of mRNA was made with TruSeq RNA Sample prep kit v.2.