GSM4407134: A33 exp5 rep2 [AT_an.A33.d356028]; Saccharomyces cerevisiae; RNA-Seq
3-5 mL samples of culture were taken, spun down at 3000rpm for 5 minutes, washed with 1 mL DEPC water, and transferred to a 2 mL RNase-free screw-cap tube. Samples were spun again at 8000rpm for 3 minutes, and supernatant was aspirated. Cells were snap frozen with liquid nitrogen and stored at -80C. RNA samples were prepared with RNeasy mini kit from Qiagen and treated with DNase on-column treatment (RNase-free) from Qiagen. Illumina Truseq or KAPA