16S rRNA amplicon sequencing of human oral microbiome: subgingival/submucosal plaque from peri-implantitis/periodontitis patient
Total bacterial genomic DNA was extracted using QIAamp DNA Mini kits (Qiagen, CA, USA) according to the manufacturer's instructions, as was previously described (Zhuang et al., 2014). PCR amplification of the hypervariable V3-V4 region of the16S ribosomal RNA (rRNA) genes was performed using the universal bacterial primer pairs, 341F (5-ACTCCTACGGGAGGCAGCAG-3) and 806R (5-GGACTACHVGGGTWTCTAAT-3). PCR amplification and library construction were performed according to Illuminas standard protocol for 16S Metagenomic Sequencing Library Preparation (Part # 15044223 Rev. B)