Illumina HiSeq 2500 paired end sequencing; RNA-seq comparison of whole muscle between WT mice, dystrophic mice, and P2rx7-null dystrophic mice
RNA-seq comparison of whole muscle between WT mice, dystrophic mice, and P2rx7-null dystrophic mice
Tibialis anterior muscle tissue was collected from 7 week old C57-BL/10, mdx and mdx/P2rx7-/- male mice. Connective and fatty tissue were removed.  Total RNA was extracted using an RNEasy plus universal mini kit (QIAGEN 73404) following manufacturer's instructions. Libraries were prepared using an Illumina TruSeq stranded total RNA kit, including ribodepletion using the Ribo-Zero Human/Mouse/Rat kit to remove ribosomal RNA.