Normalization reads (plasmid DNA) of plasmid mix with TRIP constructs, excluding PGK
For ADAMTS1, ARHGEF9, BRINP1, MED30, TMEM106B and ZNF300  promoters (excluding PGK), barcoded TRIP plasmid library was transformed into DH5-alpha E. coli and plated; 100 colonies were picked, pooled, and expanded to produce plasmid mini-libraries with 100 different barcodes each. These mini libraries were mixed together and sequenced to measure plasmid abundance. Plasmid abundance was used to normalize barcode expression.