GSM2453163: E18_20161004_Neurons_Sample_52; Mus musculus; RNA-Seq
Cortex, hippocampus, and subventricular zone were purchased from BrainBits (C57EHCV). They were from 2 E18 C57BL/6 mice dissected on the same day, shipped overnight on ice, and stored at 4C until being prepared for scRNA-Seq. Brain tissues were dissociated following the Demonstrated Protocol for Mouse Embryonic Neural Tissue, https://support.10xgenomics.com/single-cell/sample-prep/doc/demonstrated-protocol-dissociation-of-mouse-embryonic-neural-tissue-for-single-cell-rna-sequencing scRNA-Seq libraries were made following the User Guide for v2 Chemistry (https://support.10xgenomics.com/single-cell/sample-prep/doc/user-guide-chromium-single-cell-3-reagent-kit-v2-chemistry). 69 scRNA-Seq libraries were made from first mouse brain 2 days after the dissection. Another 64 scRNA-Seq libraries were made from second mouse brain 6 days after the dissection. Each library was sequenced as follows: 26bp Read 1 (16bp Chromium barcode and 10bp UMI), 98bp Read 2 (transcript), and 8bp i7 sample barcode for each scRNA-Seq library. All the samples were sequenced on 11 Illumina Hiseq 4000 flow cells, and each sample was sequenced on multiple flow cells. Samples were downsampled and aggregated with approximately 18,500 reads per cell.