GSM2723115: Diploid_F121-13_S1; Mus musculus; OTHER
Single cells from G1 or S-phase were sorted using AriaII FACS sorter based on DNA content after DAPI staining. Next, we performed whole genome single-cell sequencing as described in Baslan et al. 2012. Briefly, we performed whole genome amplification using WGA4 kit (WGA4-50RXN). Purified amplified products were sonicated to remove ends with WGA adaptors. Illumina library preparation was performed on the sonicated WGA products using NEBNext Ultra DNA Library Prep Kit for Illumina (E7370).