GSM2722435: rep1 WT ChIP; Zea mays; ChIP-Seq
Dissected 15 DAP kernels was immediately cross-linked in buffer containing 1% formaldehyde on ice under vacuum for 15 min, released the vacuum and reapplied for another 14 min. Fixation was stopped by adding 1.25M glycine. The cross-linked kernels were dried and ground into powder in liquid nitrogen, followed by nuclear isolation and sonication. Protein A-agarose beads (Millipore) and Antibody against O11 were used to precipitate the DNA. The precipitated DNA was digest by proteinase K and recovered using a QIAquick PCR purification kit (Qiagen) and analyzed by qPCR Libraries were prepared according to Illumina's instructions accompanying the TruSeq ChIP Sample Prep Kit (Catalog# IP-202-1012). ChIP DNA samples were subjected to end repair and A-base addition, followed by ligation with adapters. Then libraries werebarcoded to allow multiplexed sequencing.