GSM2264864: H9_30_1_RNA-seq; Homo sapiens; RNA-Seq
Total RNA from approximately 3-5 million cells were extracted using QIAzol lysis ralleagent (QIAGEN), and then were subjected to DNase I digestion and purified by AllPrep DNA/RNA Mini Kit (QIAGEN) according to the manufacturer's instructions. RNA-seq libraries were constructed using ScriptSeq™ v2 RNA-Seq Library Preparation kit (Epicentre Biotechnologies) according to the manufacturer's instructions.