GSM4597237: p27null_2_months.1; Mus musculus; RNA-Seq
Total mRNA was extracted from dissected pituitary intermediate lobes using the RNeasy Micro kit cDNA was generated using Ovation RNA-seq System V2 (Tecan, 7102-A01), libraries were constructed using Ovation Ultralow System V2 (Tecan, 0344NB-A01) according to the manufacturer's instructions. Libraries were quantified using the TapeStation (Agilent) and pooled in equimolar proportions. Library were sequenced on an Hiseq4000 (Illumina)