GSM2546201: H3K27me3_ChIPSeq_uninjured_rep1; Danio rerio; ChIP-Seq
cell purification protocol : Ventricles were extracted from 1-1.5 year old fish and dissociated into cell suspensions using the Miltenyi neonatal heart dissociation kit. Cardiomyocytes were strained on a 10 micrometer nylon mesh while other cell types in the flowthrough were discarded. The strainer with the attached cells was then processed as a cell pellet, including 30 minute fixation in methanol-free formaldehyde (1% final concentration) and washing with sterile PBS containing protease inhibitors. Lysates were clarified from sonicated cells and histone-DNA complexes were isolated with antibody. Libraries were prepared on a BRAVO automated liquid handling platform (Agilent) as previously described (Busby M. eta al, 2016).