GSM4406959: CD8T-antiPD1 rep2; Mus musculus; RNA-Seq
The different subtypes cells, including CD8+ T cells , T helper cells, and dendritic cells in MC38 tumors were analyzed by flow cytometry and then sorted into lysis buffer. The RNA extraction, and reverse transcription were then performed following the Smart-Seq2 protocol (S Picelli et al. Nat Methods. 2013). cDNA library construction were performed also following the Smart-Seq2 protocol (S Picelli et al. Nat Methods. 2013).