Scenedesmus bijugus
Genomic DNA was end-repaired (NEBnext ultra II end repair kit, New England Biolabs, MA, USA), cleaned up with 1x AmPure beads (Beckmann Coulter, USA). Adapter ligation (AMX) was performed for 30 minutes using NEB blunt/ TA ligase (New England Biolabs, MA, USA). Library mix was cleaned up using 0.4X AmPure beads (Beckmann Coulter, USA). Elution of the sequencing library was done with 16l of elution buffer and used for sequencing. Whole genome library was prepared by using ligation sequencing kit (SQK-LSK108) from Oxford Nanopore Technology.