GSM4474553: C59; Homo sapiens; RNA-Seq
At day 13 hiPSC-CMs were washed, dissociated and resupended in 1000 cells/uL and stored on ice for direct use after (<30min). This step was done by following the 10x genomics single cell 3' end ibrary preparation protocol. Briefly, only the 3' end of mRNA fragements were used to make library. One end of the fragements were added P5, read1 illumina sequencing primer, 16 base pairs of 10x barcode, and UMI sequences; The other end ligated with P7, sample index sequences, and illumina read2 sequencing primer sequences. scRNAseq