GSM4307796: CC_H9; Homo sapiens; RNA-Seq
Isolation of cells samples. The ES cells were washed in PBS for 2times, incubated with tryple (TrypLE™ Express Enzyme (1X), phenol red,12605028) for around 3 min at 37 °C. Total RNA was isolated from hPSCs cultured in conversional medium and AIC medium using the TRIzol Reagent (Thermo Fisher Scientific, 15596018). RNA sequencing libraries were constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB England BioLabs, E7530L). The fragmented and randomly primed 2×150-bp paired end libraries were sequenced using an Illumina HiSeq X Ten.