GSM4478071: 144-3P; Caenorhabditis elegans; RNA-Seq
mRNA was isolated from both total and polysome associated halves of the lysate using a TruSeq RNAv2 kit (Illumina) following the manufacturer's protocol.  RNA quality was assessed on an Allegiant 2100 Bioanalyzer (Agilent, Palo Alto, CA) requiring an A260/A208 > 1.7 and RIN > 8.0. All 12 samples were sent to the Jaxson laboratory (Bar Harbor, ME) for sequencing using a KAPA stranded mRNA sequencing kit (Roche), followed by 75 cycles on the NextSeq HO.