GSM2723066: Haploid_H129-2_E1; Mus musculus; OTHER
Single cells from G1 or S-phase were sorted using AriaII FACS sorter based on DNA content after DAPI staining. Next, we performed whole genome single-cell sequencing as described in Baslan et al. 2015. Briefly, the cells were lysed and amplified using the Sigma SeqPlex kit (SEQXE-50RXN). The WGA products were purified and the WGA universal adaptor sequences were removed as per the kit protocol, leaving NN overhangs. Uniquely barcoded illumina adaptors with NN overhangs were ligated to purified amplfied DNA with NN overhangs. Upto 96 uniquely barcoded cells were pooled together and re-amplified. Samples were then quantified using the Bioanalyzer and qPCR and sequenced on Hiseq 2500 sequencer using 50 bp single-end read format.