RNA-Seq of Neocalanus Flemingeri: Adult Female Week 2 S17
Double-stranded cDNA libraries were prepared from total RNA using a KAPA Stranded mRNA-seq kit (catalog No. KK8420; Kapa Biosystems, Wilmington, MA, USA) following the manufacturers instructions. Paired-end Illumina sequencing (150 base pairs [bp]) was performed on a NextSeq 500 system (Illumina, San Diego, CA, USA) using the high output flow cell with 300 cycles.