GSM4477796: Lgr5-2A-DTR 3; Mus musculus; RNA-Seq
Whole small intestines were lysed in Trizol (Qiagen). RNA was subsequently isolated with RNeasy Universal Plus kit (Qiagen) and cDNA was generated with Superscript III (Life Technologies) according to manufacturer's instructions. RNA libraries were prepared for sequencing using standard Illumina protocols