Cytochrome c oxidase subunit I (COI) amplicon sequence of mock sample containing Zophobas atratus, Acyrthosiphon pisum, Pectinophora gossypiella
Fecal samples were amplified using primers targeting the Cytochrome c oxidase subunit I (COI) in arthropods ZBJ-ArtF1c (5-AGATATTGGAACWTTATATTTTATTTTTGG-3) and ZBJ-ArtR2c (5-WACTAATCAATTWCCAAATCCTCC-3) (Zeale et al. 2011). MiniSeq sequencing was performed at the Sequencing Core (UICSQC), University of Illinois, Chicago, using a two-stage amplification protocol as described by Naqib et al. (2018).