GSM2545822: VS80 Th17_PII input; Homo sapiens; ChIP-Seq
Cells were fixed using formaldehyde solution, lysed and sonicated to obtain chromatin fragments of 100-500bp size. The sonicated chromatin was then subjected to immunoprecipitation with polyclonal anti-STAT3 antibody (rabbit polyclonal αSTAT3 9132L, Cell Signaling Technology). The STAT3 bound chromatin was isolated using Dynal magnetic beads coated with secondary antibody anti-rabbit IgG (Dynal Biotech). The immunoprecipitated DNA was eluted, reverse crosslinked and purified using Qiagen minielute columns. The sample preparation for sequencing was performed according to Illumina's recommendations by Illumina service provider, Fasteris Life Sciences, Switzerland. Having passed quality control, the library is sequenced on Illumina HiSeq 2500