Sediment of Outfall Area 1 during Summer Season
To determine the variations in bacterial community composition in response to heavy metal concentrations, marine sediment samples were collected from the Southern coastal region of India. Total community DNA was extracted using PowerSoil DNA Isolation Kit (MoBio Laboratories Inc., USA) following the manufacturer's guidelines. Metagenome from three independent extractions was combined for each sample and the quality was measured by picogreen (Invitrogen) using Victor 3 fluorometry. The hypervariable V3-V4 regions of 16S rRNA was amplified using universal bacterial primer pair tagged with unique barcode sequences. The size and quantity of prepared library was validated by running on Bioanalyzer 2100 (Agilent Technologies, California, US). The equimolar concentrations of prepared libraries were taken and sequenced on the Illumina MiSeq sequencer using 300-bp paired-end method at Macrogen Inc. (Seoul, Korea).