GSM3729252: Mo THP-1_RNA-seq [THP1_ND_3]; Homo sapiens; RNA-Seq
Total RNA was extracted using Trizol. Genomic DNA was extracted using standard phenol-chloroform method. Poly-A-enriched mRNA libraries were prepared from 2 μg of TURBOTM DNase-treated, total RNA using the TruSeq® Stranded Library Preparation Kit (Illumina), according to the manufacturers' instructions. mRNA sequencing was performed on each cell population in triplicates by Novogene (China). A total of 100 million paired-end strand-specific reads were sequenced per sample on an Illumina HiSeq 2500 Platform.Whole-genome bisulphite sequencing (WGBS) was performed on a HiSeq X Ten platform (Illumina) following library preparation using the TruMethyl WG method v.1.9 (Cambridge Epigenetix).