GSM4477421: pbmc60_mrna; Homo sapiens; RNA-Seq
PBMCs were isolated from a healthy adult using standard protocols and captured on the 10x genomics single cell RNA-seq platform 10x genomics 3' end single cell RNA-seq kit. Alterations to protocol include, following GEM generation, RT was done at 37C for 60 min, then 53 C for 45 minutes. Following silane purification of RT products, mRNA was isolated using Ampure size selection of 0.6x. Read 1 positions 1-16 contain the 16bp cell barcode, and positions 17-26 contain a 10bp UMI. Read 2 contains the cDNA.