16s rRNA sequencing of leachate AD community
Genomic Bacteria DNA of the V4 hypervariable region of the 16S rRNA gene was amplified using the barcoded primers described by Caporaso et al., 2011. These were 515f and 806r. All PCRs were performed in triplicate, using high fidelity Q5 taq polymerase (NEB) and pooled before being size selectively purified. Samples were quantified (Qubit) then pooled in equimolar concentrations before being sent to Research and Testing Laboratories (Texas) for paired-end sequencing