GSM3462417: Dl3vg3; Diachasmimorpha longicaudata; Diachasmimorpha longicaudata entomopoxvirus; RNA-Seq
Total RNA was extracted using the RNeasy Mini Kit (QIAGEN) with on-column DNase digestion, followed by a secondary DNase treatment using the TURBO DNA-free Kit after RNA isolation. Illumina-compatible stranded RNA libraries for all 12 samples were constructed with the Kapa Biosystems RNA library preparation chemistry using 3ug RNA from each fly sample and 1ug RNA from each venom gland sample. All libraries were sequenced on one run of an Illumina NextSeq instrument, which generated an average of 18.5 million and 14.2 million 75bp paired-end reads for each wasp and fly sample, respectively.