ESTs sequencing of pythium porphyrae : mycelium exposed to Porphyra 10h
GS Titanium Library Preparation kit. cDNA libraries were validated using a High Sensitivity Chip on the Agilent2100 Bioanalyzer, and emulsion PCR reactions were performed to enrich cDNA templates. Tagged libraries were combined in a picotiter plate and sequenced using a 454 Genome Sequencer FLX System