GSM4655141: Dendritic cells - D1689_uninf; Homo sapiens; RNA-Seq
24 hpi infection with MVA MOI 0.5 MDDCs were sorted according to FSC/SSC characteristics and DAPI staining, and subsequently was loaded on a 10× Chromium instrument (10× Genomics) according to manufacturer's protocol based on the 10× GEMCode proprietary technology. Single-cell RNA-Seq libraries were prepared using Chromium Single Cell 3′ v2 Reagent Kit (10× Genomics) according to manufacturer's protocol. Indexed sequencing libraries were constructed using the reagents from the Chromium Single Cell 3′ v2 Reagent Kit, following these steps: (1) fragmentation, end repair, and A-tailing; (2) size selection with SPRI select; (3) adaptor ligation; (4) post ligation cleanup with SPRI select; (5) sample index PCR and cleanup with SPRI select beads.