Genome sequencing of Parachlamydia acanthamoebae UV-7 culture: horizontal transmission regime after 14 months
Library preparation and pool sequencing of symbiont populations were performed at the Vienna BioCenter Core Facilities (VBCF) Next-Generation Sequencing (NGS) Unit (https://www.viennabiocenter.org/facilities/). Illumina Genome Analyser and HiSeq2000 instruments were used to generate paired-end reads of ~125 bases according to standard procedures, achieving the desirable coverage of 300- to 600-fold.