GSM4476856: MDM LPS sample 3; Homo sapiens; RNA-Seq
After stimulation, stimuli were removed by gently washing tissue culture plates thoroughly with HBSS for three times. MDM samples were carefully collected using a cell scraper and removing adherent MDM from the tissue culture plate. RNA was extracted using an RNA purification kit (Quick-RNA MicroPrep; Zymo Research Corp., Irvine, CA, USA) according to the manufacturer's instructions. RNA sequencing was performed using massive analysis of cDNA ends (MACE), a 3' RNA sequencing method as previously described. Briefly, 25 barcoded libraries (5 different stimuli in 5 experiments) were sequenced simultaneously on the NextSeq 500 (Illumina). PCR bias was removed based on unique molecular identifiers encoded in the library barcodes.