GSM4654312: Sam68 KO, time 0, replicate 2 [RNA-seq]; Mus musculus; RNA-Seq
In order to perform the RNA-sequencing, total RNA was extracted with the Maxwell 16 LEV simplyRNA Cells Kit (AS1270, Promega) according to manufacturer's instructions from WT, Sam68-/- and QKI -/-  EBs in three biological replicates. DNA-free RNA was then quantified using the Nanodrop and quality checked with the Bioanalizer rRNA depletion and library preparation was done with the TruSeq stranded total RNA Library prer Human/Mouse/Rat (20020596, Illumina). The sequencing was performed using 2x125bp paired-ends reads on a HiSeq 2500 sequencer with HiSeq v4 chemistry.