GSM2107199: CLIP-ALKBH1-replicate2; Homo sapiens; RIP-Seq
RNA Clean & Concentrator was used to further clean the final recovered RNA sample (Zymo Research) followed by library construction with small-RNA sample preparation kit (New England BioLabs Inc) prior to the cDNA reverse transcription. To avoid the sequencing bias, which is caused by the heavily modified status of tRNAs, we used TGIRT reverse transcriptase enzyme. Using the TGIRT reverse transcriptase enzyme, we also constructed the input library with the same procedure with the exception of the ALKBH1 immunoprecipitation step.