GSM2265815: 1_p140_Set-C; Mus musculus; RNA-Seq
For single oocyte RNA profiling Setd1bGdf9cKO and control mice were superovulated and four oocytes collected for each genotype. Cells were lysed, RNA reverse transcribed and amplified according to the protocol of SMARTer Ultra Low RNA Kit for Illumina Sequencing (vers. 1, Clontech). Samples were subjected to NEBNext Ultra DNA library preparation for Illumina using indexed adaptors (NEB). Resulting libraries were pooled in equimolar quantities for 75-bp single-read sequencing on Illumina HiSeq 2000, resulting in about 20 (16-23) million reads per sample.