Fungal ITS1 amplicons from Pleurozium schreberi stems, Reivo, Sweden, mature sections, pooled locations 1-6
Amplicons were generated with target-specific fungal ITS1 primers ITS1Fngs (GGTCATTTAGAGGAAGTAA) and ITS2 (GCTGCGTTCTTCATCGATGC), amended with Nextera XT adapter sequences on their 5' end (TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG for forward primer, GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG for reverse primer). Multiplexing was carried out in a two-step PCR approach with Nextera XT indices. Pooled amplicon libraries were sequenced on the Illumina MiSeq with the paired-end 300 bp kit.