GSM2722174: RNAseq_timecourse_miNs_day10_A; Homo sapiens; RNA-Seq
RNeasy Micro Kit (Qiagen, Cat # 74004) was used with cell pellets collected by trypsin. Total RNA was processed for library construction by Cofactor Genomics (http://cofactorgenomics.com , St. Louis, MO) according to the following procedure: Briefly, total RNA was reverse-transcribed using an Oligo(dT) primer, and limited cDNA amplification was performed using the SMARTer® Ultra® Low Input RNA Kit for Sequencing – v4 (Takara Bio USA, Inc., Mountain View, CA ). The resulting full-length cDNA was fragmented and tagged, followed by limited PCR enrichment to generate the final cDNA sequencing library (Nextera® XT DNA Library Prep, Illumina, San Diego, CA). Libraries were sequenced as single-end 75 base pair reads on an Illumina NextSeq500 following the manufacturer's instructions.