ChIP-Seq of Zea mays: MYB_TF55 Zm00001d041576 replicate 1
Transcription factor was transciently expressed in maize protoplasts with epitope tag (Avitag or HA). The protoplasts were fixed with 1% formaldehyde for 10 minutes, and lysed with lysis buffer (0.25 M Sucrose in TE with 1% Triton X-100). The chromatin was sonicated and incubated with streptavidine or Protein A dynabeads with anti-HA Ab. The biotin samples were washed 5 times with binding buffer (1 M NaCl, TE, 0.2% Tween20), twice with TE  with 0.1% SDS and twice with TE  with 0.1% Tween. The HA samples were washed with low salt buffer (0.15 M NaCl, TE, 1% TritonX100), high salt buffer (0.5 M NaCl, TE, 1% TritonX100) and lithium buffer (0.25 M LiCl, TE, 1% TritonX100). After the washing steps, on-bead Tn5 or TruSeq library prep was performed.