WGS of Strix occidentalis caurina: adult female blood
We isolated DNA using a Gentra Puregene Kit (Qiagen) and sent 41.3 µg to GENEWIZ (www.genewiz.com). We requested a barcoded mate-pair library with an insert size of 6 kb constructed using the Nextera Mate Pair Sample Preparation Kit (Illumina). GENEWIZ followed the procedure detailed in the Nextera Mate Pair Sample Preparation Guide (Illumina, Part # 15035209 Rev. C, January 2013). Traces obtained using a 2100 BioAnalyzer (Agilent Technologies) showed the center of the distribution of the sheared fragments that went into the circularization step as 7.1 kb. GENEWIZ pooled the library with 2 others equimolarly and we consequently obtained approximately 1/3 of one lane of 100 bp paired-end data sequenced on a HiSeq 2000 (Illumina).