HFD mother/HFD nursing mother
Colonic contents were extracted and genomic DNA isolated using ZR Fecal DNA miniprep (Zymo Research). Bacterial Tag-Encoded sequencing using the Ion Torrent was carried out using bar-coded primers 28F-519R for the V1V3 region of the 16S rRNA gene by the Research and Testing Laboratory (Lubbock, TX). 3000 reads per sample were obtained.