GSM4156505: SW480-siPOLR1D-2-3; Homo sapiens; RNA-Seq
RNA was harvested using Trizol reagent and isolated according to the manufacturer's instructions. Illumina TruSeq RNA Exome Sample Prep Kit (Cat#FC-122-1001) was used with 1μg of total RNA for the construction of sequencing libraries. More specifically, total RNA libraries were prepared with TruSeq RNA Library Prep for Enrichment kit (Cat. No. 20020189) and transcriptome enrichment was performed with TruSeq RNA Enrichment kit (Cat. No. 20020490),