GSM4476160: RNA-Seq_DeltatrcRrep2; Caulobacter vibrioides NA1000; RNA-Seq
First, cells were lysed in a Ready-Lyse lysozyme solution (Epicentre Technologies) according to manufacturer's instructions and lysates were homogenized through QiaShredder columns (Qiagen, 79654). RNA was then purified on RNEasy kit column. To remove DNA from samples, a first treatment using the On-column DNase I digestion kit (Qiagen, 79254) was performed and RNA was extracted according to manufacturer's instructions. Extracted RNA were subjected to a second DNase treatment Using Promega RQ1 at 1 unit/mg RNA. After phenol/chloroform/isoamyl (Sigma) extraction and ethanol precipitation, the total extracted RNA was submitted to the iGE3 sequencing facility at the Genomics Platform in the CMU (University of Geneva, Faculty of Medicine) for library preparation, next generation sequencing and differential expression analysis. The total extracted RNA was submitted to the iGE3 sequencing facility at the Genomics Platform in the CMU (University of Geneva, Faculty of Medicine) for library preparation, next generation sequencing and differential expression analysis.The total RNA subjected to library preparation using TruSeq ribodepleted Stranded mRNA (SR100-Illumina Hiseq4000) according to manufacturer's protocols.