GSM3819675: E14.5 Sox2-EGFP+ cochlear duct cells rep 1; Mus musculus; ATAC-seq
Cochlear ducts were microdissected from E12-E16 temporal treated with collagenase and dispase. Cochlear ducts were dissociated using the Worthington papain dissociation kit. Sox2-EGFP+ and Sox2-EGFP- cells were sorted by FACS. Libraries were prepared from up to 50,000 cells using the Illumina Nextera kit as described by Buenrostro et al. Curr Protoc Mol Biol. 2015.