RNAseq of Capra hircus: mucosa and lymph node infected by Haemonchus contortus and controls
poly-A mRNA was purified from 4g of total RNA, fragmented and randomly primed for reverse transcription to generate double stranded cDNA. The cDNA fragments were then subjected to an end repair process, consisting of the addition of a single A base, and the ligation of indexed Illumina adapters at both ends of cDNA.