CD49f-low 3
Total RNA from each sample was prepared using TruSeq RNA sample prep reagents (Illumina, San Diego, CA, USA) according to manufacturer's instructions, with fragmentation for 4 min at 94C. The amplified fragmented cDNA of 300 bp in size were sequenced in paired-end mode using the Illumina HiSeq 4000 with a paired end read length of 150 bp and two FASTQ files were generated for each sample.