GSM4155837: MNase_2h_B; Saccharomyces cerevisiae; MNase-Seq
Cells were fixed with 1% formaldehyde for 15 minutes at RT with mild shaking. Crosslinking was halted with 125 mM Glycine for 5 minutes. Cells were washed with PBS and the pellet was flash frozen in liquid nitrogen. Cells were spheroblasted with zymolase and the DNA was digested with MNase as in (Rodriguez et al. 2014) with minor modifications as outlined in our methods.