GSM3417048: TriplexRNASeq_SPRI_rep3; Homo sapiens; OTHER
Chromatin was purified and chromatin-associated nucleic acids were isolated. Following the fragmentation of RNA, long DNA and DNA-associated RNA were separated from free RNA by Solid Phase Reversible Immobilization (SPRI)-based paramagnetic bead size selection. Alternatively, DNA-associated RNA was immunopurified using Anti-DNA antibody (DNA-IP). Recovered RNAs from biological replicates were subjected rRNA depletion (except DNA-IP samples) using the NEBNext rRNA depletion kit (NEB). RNA libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit and NEBNext Multiplex Oligos for Illumina (NEB).