GSM2454184: 75M,MAB at MOI of 1000:1,at 3 days after infection; Homo sapiens; RNA-Seq
Infected and uninfected control respiratory epithelial cells were washed 3 times with PBS and total RNA was extracted using RNeasy Mini kit and treated with DNase I. Illumina TruSeq Stranded mRNA Sample Preparation Kit with 750ng of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols (Truseq Stranded mRNA LT kit)