Human genome NA12828 was sheared to 1.5kb and a mate-pair library was generated with two 25-bp tags (2x 25bp). The NA12828 was seeded into emulsion PCR for production of clonal template beads. Approximately 300 million template beads (representing two, 25-base paired tags) were deposited per slide, and 2 slides were used for each instrument run. The NA12828 experiment represents a single SOLiD instrument run (2 slides) where each 25-base tag (50 bases total) was sequenced to give rise to two 25bp tags with a linkage of 1.5kb for the paired tags.