RNA-Seq of Maize Autophagy Mutants under Fixed Carbon Starvation
TruSeq v2 mRNA Sample Preperation: 1 ug RNA, polyA seletction, fragment, prime, finish mRNA, 1st and 2nd strand cDNA synthesis, purification , end-repair, adenylate 3' ends, indexed adapter ligation, purify, PCR amplification (10 cycles), purify, quantification/ATTI Fragment Analyzer verification.