GSM4474639: g_DE163_Cb_rep1; Escherichia coli; OTHER
Genomic DNA was extracted from exponentially grown E. coli cells harvested at OD 600 =1.0 according to the published protocol (He, F. E. coli Genomic DNA Extraction Bio-101, e97, doi:10.21769/BioProtoc.97  (2011)) Preparation of genomic DNA libraries was carried out using the NEBNext Ultra II FS DNA Library Prep Kit (NEB) according to the manufacturer's instructions. The approximate insert size was selected to be in the range of 150-250 bp. Barcodes were introduced to both ends during library amplification step with NEBNext multiplex oligos for Illumina (NEB). Genomic DNA libraries were sequenced using HiSeq2500 platform (Illumina) in the rapid run mode (50 nt single end reads).