GSM3205673: axo16.E5; Ambystoma mexicanum; RNA-Seq
Single cell isolation and cDNA generation using Fluidigm C1 platform with reagents provided by Fluidigm as well as the SMARTer v2 Ultra Low RNA kit for Illumina (Clontech). External RNA spike-in transcripts (ERCC spike-in Mix, Ambion) were added to all single cell lysis reactions at a dilution of 1:40,000. Libraries were prepared using Illumina Nextera XT kit according to illumina's protocol.