GSM4039538: Ume6_E-ChRP_13hr_RAF_MNase; Saccharomyces cerevisiae; MNase-Seq
cells were crosslinked with 1% formaldehyde for 20 min, spheroplasted, digested with micrococcal nuclease, crosslinks reversed overnight at 65 degrees with proteinase K treatment, phenol-chloroform extracted, RNase-treated, mononucleosome band gel-extracted and CIP-treated CIP-treated nucleosome-sized DNA footprints were converted to libraries with NuGEN Ovation Ultralow kit