GSM2546192: PMCs isolated from 24 hpf embryos, replicate 2; Strongylocentrotus purpuratus; ATAC-seq
Nuclei were extracted using lysis buffer washes and ATAC-seq was performed: 150,000 nuclei were digested with 2.5 ul Tn5 transposase and a Nextera library was created. Nextera library kit used to generate Illumina sequencing library for NextSeq