L. donovani  BPK282 hamster derived amastigotes
One microgram of genomic DNA was used for bisulfite conversion with innuCONVERT Bisulfite All-In-One Kit (Analytikjena). Sequencing libraries were prepared with the TruSeq DNA Methylation kit according to the manufacturers instructions (Illumina). The resulting libraries were paired-end (2 x 100bp) sequenced on the Illumina HiSeq 1500 platform of the University of Antwerp (Centre of Medical Genetics).