GSM4478028: 43_MF1; Homo sapiens; RNA-Seq
RNA was extracted using TRIzol Reagent (Thermo Fisher) at 4 and 24 h post-infection and purified using RNeasy MinElute Cleanup Kit (Qiagen, The Netherlands). The concentration and purity of RNA was evaluated by NanoDrop 2000 (Thermo Fisher). RNA libraries were prepared for sequencing using standard Illumina protocols Illumina protocols were used for RNA-seq libraries using the Illumina TruSeq RNA Sample Preparation Kit v2 (Illumina,Inc., San Diego, CA, USA). The manufacturer's instructionswere followed with the exception of two modifications. Inthe adapter ligation step, 1ml, instead of 2.5ml, adaptor was used.