GSM4656103: Donor102_control; Homo sapiens; RNA-Seq
Human islets were received in 30 ml HEPES buffered media. Islets were transferred to a 50 ml tube and centrifuged at 150 rcf (g) for 2 min at RT. Supernatant was removed and cells were washed once with 5 ml of Accutase.  Another 5 ml of Accutase was added for incubation at 37 C for 8-10 min with pippeting up and down every other minute. After incubation 5 ml of cold islet media was added and cells were suspended by pipetting. Cells were passed through a 40 um cell strainer and centrifuged at 500 g for 5 min. Cells were washed twice with PBS, 500g and 5 min. Finally, cells were re-suspended in HBSS and keep on ice before FACS. Single-cell were FACS-sorted into 384 well plates. Smart-seq2 (Picelli et al. Nature Methods 2013)