GSM3669779: P2_F7_exp1_K562_60min4sU; Homo sapiens; RNA-Seq
NASC-seq: Cells were distributed to NASC-seq lysis buffer with FACS. TT-seq: Total RNA was extracted using TRIzol (Life Technologies), 4sU-labeled RNA was extracted according to Dolken et al., RNA, 2008 and fragmented with a BioRuptor Next Gen (Diagenode) at high power for one cycle of 30''/30'' ON/OFF (see also Schwalb et al., 2016) NASC-seq: Libraries were prepared using the NASC-seq protocol TT-seq: Sequencing libraries were prepared with the Ovation Universal RNA-Seq System (NuGEN) following the manufacturer's' instructions