GSM3728729: NctA-S_NoDrug_ChIP_Rep2; Aspergillus fumigatus; ChIP-Seq
Cross-linked mycelia were ground to a fine powder under liquid nitrogen. Samples were suspended in a ChIP lysis buffer (50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% Deoxycholate (Sigma D6750), 0.1% SDS, 1 mM PMSF, and fungal proteinase inhibitor cocktail (Sigma)), and then sonicated with a Q125 sonicator (Qsonica, USA). The sheared chromatin was immunoprecipitated with a GFP-polyclonal antibody (A-11122, Thermo Fischer Scientific) on Dynabeads Protein A magnetic beads (Thermo Fischer Scientific). Immunoprecipitated DNA was reverse cross-linked, treated with RNase A (Sigma), and then purified using a MinElute PCR purification kit (QIAGEN). Libraries were constructed following the manufacture's instractions for Illumina TruSeq ChIP-seq samplepreparation kit.