GSM4407245: P3; Homo sapiens; RNA-Seq
Cells were scraped out in 1xDPS from the transwell, spun 10000 rpm @4C to have cell pellet. The cell pellet was incubated with RLT + BME buffer for a minute, transfer into a Qiashredder, spun 15,000 rpm for 3 min @4C, collected the elusion and stored -80C. QIAGEN total DNA/RNA kit was used for RNA extraction including DNase treatment according to the protocol. RNA-Seq libraries were prepared using the NEBNext Ultra II nondirectional Ribodepletion kit