RNA-Seq of crustose coralline algae: Lithophyllum sp.
Samples for molecular analysis were taken from 7 varying treatment conditions to obtain well represented transcriptomes. RNA was extracted from the epithallus of the algae using a modified TRIzol extraction protocol. RNA extracts were pooled for each species, quantity and quality checked, and sent to Macrogen, Inc (Seoul, South Korea) for CDNA library preparation using a TruSeq Stranded mRNA LT Sample Prep Kit.