DNA-Seq of Pyxicephalus adspersus: adult female oviduct 20 kb mate-pair library
Nuclei were extracted by gently homogenizing oviduct tissue with a glass dounce in 1X Dulbeccos Phosphate Buffered Saline. The lysate was filtered in miracloth and washed with buffer to release trapped nuclei. Nuclei were pelleted and placed into agrase using Bio-Rad plug molds. DNA was freed from molds and sheared to ~20 kb lengths and fragmented DNA will be used to construct libraries following Lucigen s protocols for the NxSeq Long Mate Pair Library Kit.