16s rRNA gene survey of kakapo fecal samples
DNA was extracted using a bead-beating, CTAB and chloroform extraction method (author can be contacted for details). The variable V3-V4 region of the bacterial 16S rRNA gene was amplified using primers 341F and 806R with Illumina adapters. Sequencing was performed by New Zealand Genomics Ltd, using the Illumina MiSeq platform (2x300 bp chemistry). Library ID corresponds to the name of the invididual bird from which the fecal sample was collected, with a number appended to distinguish replicate samples from the same bird.