GSM2366938: WT_rep2; Danio rerio; RNA-Seq
Zebrafish were grown at standard conditions and flash frozen in liquid nitrogen at 4dpf, RNA was harvested using Trizol reagent. rRNA was depleted from 20µg total RNA using RiboMinus™ Eukaryote Kit v2 A15020 (Ambion/Life Technologies). TruSeqIllumina RNA sample prepv2 kit  Part # 15026495 was used for RNA library preperation using standard Illumina protocols