GSM4656253: SOPR0476; Homo sapiens; RNA-Seq
Single cell suspensions of ileal biopsy tissue were co-encapsulated with genetically-encoded beads (Drop-seq). Monodisperse droplets at 1 nl in size were generated using the microfluidic devices fabricated in the Centre for Hybrid Biodevices, University of Southampton. Following encapsulation, approximately 1000 STAMPS (beads exposed to a single cell) for each donor were taken further for library prep. The resulting libraries were run on 2 shared NextSeq runs (1.5x105 reads for maximal coverage) at the Wessex Investigational Sciences Hub laboratory, University of Southampton, to obtain single cell sequencing data.