GSM2263693: H3-ChIP 25°C biological replicate A; Arabidopsis thaliana; ChIP-Seq
For each sample, 0.5 g of plant material were used for each biological replicate. Material was collected at from plant growing at 16 °C, as well as at 1 day after the plants were moved to 25°C (5-6 h after lights on). Using jeweler's forceps, leaves were removed to obtain SAM enriched tissue. ChIP experiments were performed following a previously published protocol (Kaufmann et al., Nature Protocols 2010). After the preclearing step the sample was split, one aliquot was incubated with anti-H3K36me3 antibody (ab9050, abcam)and the other with anti-H3 antibody (ab1791, abcam). Samples were prepared for Illumina sequencing using the ThruPLEX DNA-seq Kit (Cat. No: R400429) from Rubicon Genomics according to the manufacturer's protocol.