GSM4041299: control1; Homo sapiens; ncRNA-Seq
Total RNA was isolated from the exosomes using TRIzol LS Reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions, and quantified using the NanoDrop ND-1000. RNA libraries were constructed by using NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England Biolabs, Inc., Massachusetts, USA) according to the manufacturer's instructions. Libraries were controlled for quality and quantified using the BioAnalyzer 2100 system (Agilent Technologies, Inc., USA).