GSM4654193: WZ28; Homo sapiens; RNA-Seq
Total cellular RNA was extracted using TRIzol (TaKaRa, Tokyo, JPN). RNA degradation and contamination were monitored on 1% agarose gels. RNA concentration was measured using Qubit® RNA Assay Kit in Qubit® 2.0 Flurometer (Life Technologies, CA, USA). RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA) RNA libraries were prepared for sequencing using standard Illumina protocols