Normalization reads (genomic DNA) of ZNF300 TRIP pool A, replicate 1
Protocol by Akthar et al. 2014 (Nature protocols) was used to quantify barcode abundance. 100 ng of gDNA was used as a template for amplification of barcode sequences (two PCR replicates were performed each with a different index). PCR was performed in two rounds and purified products were directly used for high throughput Illumina single-read sequencing.