16S V4 EMP Amplicons of near-shore sandy sediments incubated with weathered crude oil over 3.5 months
Total genomic DNA was extracted from 0.25 g of sand in triplicate from each chamber at each major sediment sampling point with a MoBIO PowerSoil DNA Extraction kit following the manufacturers protocol. For the 16S SSU rRNA gene amplicon libraries, the triplicate extracts were amplified using the Earth Microbiome Project primers (515F / 806R). The triplicate PCR products for each sample were pooled in equimolar amounts, and the resultant mix was indexed.