Rice (CL 151) inoculation with B. glumae at the seedling stage
Total RNA extracted from inoculated rice seedlings was divided in half. One half was enriched for the rice transcriptome using polyA selection while the other half was enriched for the bacterial trancriptome via the following steps: 1) eukaryotic rRNA removal; 2) prokaryotic rRNA removal and 3) removal of polyA transcripts.  Resulting transcripts (one for rice and one for B. glumae) were size-fractionated (100-300 bp) and amplified using random primers via Illumina chemistry.  Three biological replicates with each biological replicate consisting two technical replicate were generated.  Water-inoculated controls were also generated for the rice transcriptomes consisting of three biological replicates with each biological replicate consisting of two technical replicates.