GSM2876217: CD271_pos_1; Homo sapiens; RNA-Seq
Sorted CD271+ and CD271- cells were collected and RNA was isolated using the Norgen RNA extraction kit (Norgen Biotek) according to manufacturer's instructions for mammalian cultured cells. Libraries were prepared from 500 ng of input total RNA with the Truseq RNA v2 library prep kit (Illumina).  DNA libraries were prepared with unique barcodes and pooled; 75 cycles of single-end sequencing were performed on a high output flowcell with the NextSeq 500 (Illumina).