Compensatory cis-trans regulation of mouse liver gene expression
Compensatory cis-trans regulation of mouse liver gene                 expression
All mice used in this study were housed and handled                     in accordance with the Animals (Scientific Procedures) Act 1986. Mice were                     housed and cared for in the Cambridge Research Institute Biological Resource                     Unit with a twelve hour light/dark cycle, and were provided with chow food                     from Lab Diet plus water ad libitum. Mice were sacrificed by cervical                     dislocation at 4-6 months of age, between the hours of 9:30am and 11:30am. The                     liver was then cut and the mouse perfused with PBS by injection into the                     heart. Subsequently, the liver was removed and the gall bladder and hepatic                     vein cut away. A sample of approximately 50mg-100mg was taken from a single                     liver lobe and frozen on liquid nitrogen for storage at -80C until use. For                     each liver sample, total RNA was extracted using the RNeasy Mini kit (Qiagen                     74104) as per manufacturerÃ¢â‚¬â„¢s instructions. Before further processing,                     each total RNA sample was treated to digest contaminating DNA. From each                     sample, polyadenylated mRNA was enriched from the total RNA using the                     PolyATract mRNA isolation system.