WGS of Strix occidentalis caurina: adult female blood
We isolated DNA using a Gentra Puregene Kit (Qiagen) and used 50 ng to prepare a genomic library using a Nextera DNA Sample Preparation Kit (Illumina). After tagmentation, we cleaned the reaction with a DNA Clean & Concentrator -5 kit (Zymo Research). We amplified the reaction for 5 cycles of PCR using a KAPA Library Amplification kit (KAPA Biosystems) and then cleaned the reaction with a DNA Clean & Concentrator -5 kit (Zymo Research). We used a BluePippin (Sage Science) to select library fragments in the size range of 734-934 bp, which, after subtracting the 134 bp of adapters, corresponded to selecting an average insert size of 700 bp. We performed a real-time PCR (rtPCR) using a KAPA Real-Time Library Amplification Kit (KAPA Biosystems) on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad) to amplify the library. We amplified the library with 6 cycles PCR and then cleaned the PCR products with a DNA Clean & Concentrator -5 kit (Zymo Research). We obtained one lane of 150 bp paired-end data sequenced on a HiSeq 2500 (Illumina) in rapid mode.