GSM4105346: SK-N-BE(2)-C H3K4me3 histone-ChIP-Seq; Homo sapiens; ChIP-Seq
For ChIP-Seq, cells were crosslinked, flash frozen, and sent to Active Motif. According to Active Motif protocol, cells were lysed, sonicated, and then DNA-protein complexes were isolated with antibodies. Samples were reverse-crosslinked and DNA was purified. For ATAC-Seq, whole cells were frozen and sent to Active Motif for thawing and transposition. Illumina libraries were prepared by Active Motif using standard consecutive enzymatic steps of end-polishing, dA-addition, and adaptor ligation. After 15 cycles of PCR amplification, the resulting DNA libraries were quantified and sequenced by Jefferson Cancer Genomics Laboratory at the Kimmel Cancer Center using the NexSeq 500.