454 GS FLX Titanium sequencing
DNA was extracted from 0.5 g soil samples and a 520 bp fragment of the V2 - V3 16S rRNA operon was amplified in triplicate from each soil sample using the PCR primers 27F (5�-AGAGTTTGATCCTGGCTCAG-3�) and 533R (5�-TTACCGCGGCTGCTGGCAC-3�).