GSM3132656: AP2-FG ChIP-seq experiment-1 INPUT; Plasmodium berghei ANKA; ChIP-Seq
The infected whole blood was fixed immediately with formalin (1% in final concentration), and erythrocytes were depleted by lysis in 0.85% NH4Cl with changing the solution several times. ChIP was performed according to the protocol in “Standard protocols on Next Generation Sequencing” (edited by Ai Nikaido, Yodosha Co., Ltd., 2014). Briefly, chromatin was sheared by sonication using Bioruptor. Chromatin was IPed with anti-GFP polyclonal antibodies (Abcam) conjugated to Dynabeads Protein A (Life Technologies, Gaithersburg, MD).  Obtained DNA fragments were sequenced by the illumina NextSeq sequencer. Single read - illumina NextSeq 500