GSM2877317: 575A RXRA WT DMSO 1; Homo sapiens; RNA-Seq
RNeasy Mini Kit (Qiagen) was used to isolate RNA from cell pellets Ribosomal RNA was removed by poly-A selection using Oligo-dT beads. mRNA was then fragmented and reverse transcribed to yield double stranded cDNA using random hexamers. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 12 cycles using primers incorporating unique index tags.