RNA-Seq of Salmo trutta: PKD infected juvenile pelvic fin
Total RNA was extracted from pelvic fin tissue using NucleoSpin RNA kit and ensuring RNA quality using the Agilent 2100 Bioanalyzer. Three barcoded libraries, using Lexogen QuantSeq 3 mRNA-Seq Library Prep Kit FWD for Illumina were prepared, according to manufacturer's recommendations (Lexogen, Vienna, Austria). The three pooled barcoded libraries, consisting of 64, 91 and 96 individuals, were single-end sequenced using Illumina HiSeq2500 in 14 lanes (one library per lane). For the first two lanes, we generated 125 bp long reads and for the remaining twelve lanes, we generated 100 bp reads. This entry belongs to the pool consisting of 64 individuals and 18 PCR cylces were used for it.