GSM3415943: MCF7_8h_2g_S15; Homo sapiens; RNA-Seq
Pellets of up to 1x10^7 cells were collected by trypsinization at 0, 2 and 8 h post-radiation, snap-frozen on dry ice and stored at – 70oC for subsequent RNA extraction. Total RNA was extracted from cells with the RNeasy Mini Kit using QIAshredder technology (UK Qiagen, Ltd). The manufacturer's protocol for purification of total RNA from animal cells using spin technology was followed. Lexogen QuantSeq 3' mRNA-Seq Prep Kit for Illumina (FWD)