GSM3209374: I10_THZ1_50NM_1; Homo sapiens; RNA-Seq
Total RNA was isolated using the Rneasy Plus Mini (Qiagen Cat# 74134) and ERCC spike in controls (Life Technologies, Cat 4456740) were added after total RNA extraction (1ul to 5000ng RNA). Library prep was conducted using TruSeq Stranded mRNA Library Prep Kit (Illumina Cat #RS-122-2101/2) according to manufacture instruction. RNA sequencing was done on HiSeq 2500v4 high output (50-bp, single-end reads).