GSM3209082: P4PM: Melanoma primary tumor; Homo sapiens; RNA-Seq
Total RNA were extracted using the Qiagen's AllPrep Mini Kit according to supplied protocols. Quality and quantity of samples were measured with Nandrop, gel electrophoresis and Qubit High Sensitivity Kit. RNA integrity was determined using the Agilent Bioanalyzer Nano chip. Library preparation was performed using Illumina's TruSeq Sample Prep Kit, amplification was done on Illumina's cBot Cluster Generation System. Paired-end reads (2x 83 bp) were generated on the Illumina HiSeq 2000