WGS of Legionella pneumophila subsp. pneumophila: hot water system
Strains were isolated from drinking water obtained from branch lines serving a hot water system.  A 100 mL aliquot of each sample was cultured on Buffered Charcoal Yeast Extract (BCYE) agar with antibiotics and screened by latex agglutination test.  DNA was extracted using the UltraClean DNA Microbial Isolation kit.  Paired-end 150 libraries were prepared using the Nextera XT DNA library kit followed by rapid mode sequencing on the HiSeq 4000 platform.