GSM2454252: VM_7w_a; Homo sapiens; RNA-Seq
Human fetal tissues were collected from routine termination of pregnancies at Addenbrooke’s Hospital (Cambridge) and dissected in HIBERNATE media. Ethical approval for the use of postmortem human fetal tissue was provided by the National Research Ethics Service Committee East of England - Cambridge Central (Local Research Ethics Committee, reference no. 96/085).  Tissue pieces were dissociated using Papain Dissociation System (Worthington) following the manufacturer’s recommendations, adjusting incubation time based on tissue piece size, 25-45 min. Glass pipettes of increasingly smaller tip diameter were used to dissociate to single-cell suspension followed by a centrifugation through a BSA single step discontinuous density gradient. Then cells were filtered with 20 μm strainer (Partec CellTrics). Cells were pelleted, resuspended, and stored in N2 media with DNaseI until use. Approximately 800 cells were used from each sample.