Whole genome sequencing of amniotic fluid cell-free DNA from full term pregnancy: Sample HUZ286696.AF
Short cell-free DNA molecules were extracted from urine supernatants and ligated to a biotinylated adapter. Molecules were then bound to magnetic beads and end repair and ligation to a double stranded sequencing primer were performed. DNA libraries were released from bead via denaturation. Libraries were then amplified and indexed using PCR.