14-S-108128
DNA was extracted from formalin-fixed paraffin-embedded (FFPE) sections of the initial breast cancer primary with at least 50% tumour percentage from 110 patients. DNA libraries were generated using the TruSeq Nano Library Preparation kit (Illumina). Hybridization capture to a customised Agilent SureSelectXT panel was used to enrich coding regions from 567 cancer-related genes. A total of 63 unique samples passed quality control metrics for sequencing on a HiSeq-2500 with average coverage of at least 300X and were included in the analysis. The NGDx PanCancer version 2 panel interrogates the entire coding sequence of 567 cancer-related genes plus select introns from 57 genes often rearranged or altered in solid tumor cancers.