GSM2546908: Ctrl_T7d.1; Mus musculus; RNA-Seq
For samples 1-3, mouse back skin was incubated on 0.25% Trypsin for 2 hours at RT and then separated epidermis from dermis. The epidermis was finely chopped and transferred to DMEM with 10% FBS and stirred for 20min to get single cells. For samples 4-5, melanoma tumor was harvested and finely chopped and incubated in DMEm with 10%FBS and 0.35%  Collagenase I for 35min to get single cells. Then, total RNA were isolated from all samples using RNeasy Plus Micro-Kit (Qiagen) as described by manufacturer. RNA libraries were prepared for sequencing using standard Illumina protocols