GSM3416546: AGS-H3K27me3; Homo sapiens; ChIP-Seq
ChIP was performed using antibodies: H3K27ac (ab4729, Abcam), H3K4me1 (ab8895, Abcam). After recovery of ChIP and input DNA, whole-genome-amplification was performed using the WGA4 kit (Sigma-Aldrich) and BpmI-WGA primers. Amplified DNAs were purified using PCR purification columns (QIAGEN) and digested with BpmI (New England Biolabs) to remove WGA adapters. 30ng of amplified DNA was used for each sequencing library preparation (New England Biolabs). H3K27me3 ChIP was performed by Zymo Research (ab6002, Abcam). ChIP-seq libraries were prepared following manufacturer protocols (New England Biolabs).