GSM3415922: M2 rep2; Homo sapiens; RNA-Seq
Trizol reagent (Invitrogen) was used to extract total RNA according to manufacturer's protocol. The concentration was measured using Nanodrop instrument (ThermoFisher SCIENTIFIC). RNA was treated with RNase-free DNase I (Sigma, USA). 8 mRNA-seq samples were pooled and sequenced on HiSeq using Illumina TruSeq mRNA Prep Kit RS-122-2101 and paired-end sequencing.