HepaRG_DENSpm_rep3.fastq.gz
Total RNA was isolated from the cell lines using a MagNA Pure Compact RNA Isolation Kit (Roche, Switzerland) on a MagNA Pure Compact Instrument (Roche). The quality of isolated RNA was determined by an Agilent 2100 Bioanalyzer (Agilent Technologies, USA). The RNA with RIN (RNA Integrity Number) values of more than 8 was used only. Quantification of RNA was performed with a Qubit 2.0 Fluorometer (Thermo Fisher Scientific, USA). The cDNA libraries were prepared using a TruSeq RNA Library Preparation Kit v2 (Illumina, USA) according to the manufacturers instructions. Total RNA was purified using oligo-dT attached magnetic beads. Following purification, the mRNA was fragmented and then converted to double-stranded cDNA. The cDNA was subjected to ligation of the adaptors and amplification. The quality of final cDNA libraries was evaluated by qPCR on a Rotor-Gene Q Instrument (Qiagen, Germany).