GSM4308694: S07: mRNA_Male_Neu_07; Homo sapiens; RNA-Seq
Peripheral blood was collected in heparinized tubes after venipuncture. Neutrophils were isolated at room temperature by density gradient  followed by lysis method. For RNA purification, neutrophils were centrifuged at 1600rpm for 5 minutes and resuspended in 750 μL TRI reagent (Sigma, cat. no. 93289). RNA was isolated using Zymo Research Direct-zol RNA Miniprep kit (Zymo Research, cat. no. R2050) cDNA libraries were prepared using the TruSeq® Stranded mRNA NeoPrep™ Kit (Illumina, cat. no. 20020594) RNA seq data were generated on Illumina HiSeq 3000 instrument according to the manufacturer's instructions