GSM2719785: Control 2; Homo sapiens; RNA-Seq
RNA was isolated from frozen whole rectal wall samples with the RNAeasy Mini Plus Kit (Qiagen, Hilden, Germany) and checked for quality and quantity with Agilent’s 2100 Bioanalyzer using the RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, USA). cDNA libraries were prepared using Illumina’s TruSeqTM RNA Sample Preparation v2 protocol. Sequencing was performed using an Illumina HiSeqTM 2500. Quality and quantity was checked with Agilent's 2100 Bioanalyzer using DNA 1000 Kit.