GSM2546897: T1_nt_r2; Candida albicans; RNA-Seq
Total RNA was extracted after 1 h or 3 h tomatidine/solvent exposure by mechanical disruption of the cells with glass beads. Experiments were carried out in triplicates with 12 samples Total RNA extracts were treated with DNase using the DNA-free kit (Ambion-Life Technologies, Zug, Switzerland) and RNA quality and integrity was verified with Fragment Analyzer™ Automated CE System (Advanced Analytical). One µg of RNA was used to create sequencing libraries through standard Illumina TruSeq stranded mRNA protocol. Each library (sample) received a different index enabling several libraries to be multiplexed. Before RNA sequencing, libraries were analyzed with a fragment analyzer to assess quality and fragment size and with a Qubit fluorometer (Invitrogen) to determine cDNA concentration. Libraries were kept at −20°C until sequencing. The 12 libraries were run on Illumina HiSeq platerform (HiSeq2500)