GSM3729314: Act_HPV8_rep3; Mus musculus; RNA-Seq
Total RNA was isolated immediately after sorting using the RNeasy Micro Kit (Qiagen, Hilden, Germany) and stored at -80°C. RNA was analyzed via TapeStation (Agilent), and samples with RIN>7 were adjusted to 100 ng and subjected to the RNA sequencing protocol via poly-A enrichment, True-Seq library preparation, and stranded 100bp sequencing on an Illumina HiSeq 2500 instrument. Quality control via FastQC showed rRNA mapping rates of <5% and over 30 million reads for all samples. RNA libraries were prepared for sequencing using standard Illumina protocols (poly-A enrichment, True-Seq library preparation)