1h post infection by Vertillium dahliae
Total RNA was isolated from the frozen tissues using an RNA Extraction Kit (YPHBio, Tianjin, China) following the manufacturer  s instructions, including an on-column DNase I digestion. The mRNA was further purified by oligo(dT) magnetic beads and magnet separator. The quality and concentration of mRNA were determined by Agilent 2100 Bioanalyzer (Agilent, California, America). Then, the mRNA was broken into small fragments under heating conditions, which were reverse transcribed into first strand cDNA with random hexamer primers. The second strand cDNA was synthesized and sequenced using HiSeq 2000 (Illumina, America).