GSM2453193: CENPB-library-fixed-length-cleaved-HeLa; Homo sapiens; RNA-Seq
One day after transfection of a plasmid library, cells were harvested for total RNA isolation (RNeasy Mini Kit, Qiagen) RNA was reverse transcribed using SuperScript III (Life Technologies) and an anchor dT primer, and the RT products were amplified using Phusion HF and a forward primer that hybridized upstream of the query region