16S rRNA V3-V4 region amplicon of human urinary tract metagenome: male urine
DNA was extracted using the Bacteremia DNA isolation Kit (MoBio). The V3-V4 region of the 16S rRNA gene was targeted for broad-range PCR with Illumina MiSeq sequencing for characterizing the bacterial biota. Nextera XT Index kits v2 (Illumina) were used to facilitate multiplexing. Reactions were purified using Agencourt AMPure beads prior to sequencing.