Jungle carpet python virus-infected lung, spleen, and brain library
RNA was extracted from pooled lung, spleed, and brain tissue from a JCPV-infected carpet python (Morelia spilota). The RNA extract was depleted of ribosomal RNA (rRNA) using the RiboMinus Eukaryote System v2 (cat. no. A15026, Ambion, Thermo Fisher, Victoria, Australia) following the manufacturer s recommendations. The DNase-treated rRNA-depleted total nucleic acid extracts were then submitted to the Australian Genome Research Facility (www.agrf.org.au) for next-generation sequencing using Illumina HiSeq 2500 using 150 base pair, paired-end (PE) reads.