GSM2721960: GRC303_94; Rattus norvegicus; miRNA-Seq
Serum and EDTA plasma samples were collected from the abdominal aorta. 100 µl of serum thawed on ice were centrifuged at 3000 X g for 5 min. Small RNA was isolated using miRCURY RNA Isolation Biofluids Kit following manufacturer's instructions, including an optional on-column DNase digestion (Exiqon, Woburn, MA). Indexed small RNA sequencing libraries were prepared using CleanTag Small RNA Library Prep Kit (TriLink BioTechnologies, San Diego, CA) with 10 ng of purified input RNA following manufacturer's instructions. Libraries were quantified using Bioanalyzer High-Sensitivity DNA chip, pooled, and were sequenced using a NextSeq 500 sequencer on High Output mode (Illumina, San Diego, CA) using 50 cycles of single-end reads.