Laser capture microdissection of Zea mays subsp. mays inbred B73, 10 plant pool, P6 primordia, lg2-R Rep2
2-week old maize (inbred B73) plants were dissected down to small, 5mm x 2.5mm x 1.5mm cassettes, fixed in 3:1 ethanol and acetic acid, dehydrated, parafin infiltrated, and sectioned for laser capture microdissection. Leaf primoridum tissue was harvested in a pool of 10 individuals, comprising greater than 2 million um^2. RNA was extracted from captured tissue and amplified with the Arcturus RiboAmp 2-round linear RNA amplification kit. Amplified RNA was prepared for Illumina RNA sequencing and sequenced with 2 x 100 bp chemistry on an Illumina HiSeq2500.