GSM3591436: Q11_3D_3; Rattus norvegicus; RNA-Seq
Total  cellular  RNA from PC12 cell lines expressing pcDNA3 (EV) and expressing pcDNA3-α1ACT(α1ACT) was isolated by RNeasy mini Kit (QIAGEN, Valencia, CA, USA) according to the manufacturer's instructions at expected time points. PC12 cell lines expressing pcDNA3 (EV) and expressing pcDNA3-α1ACT at 4 different time points (6h, 24h, 3d, 10d) were analyzed by Agilent Bio-analyzer and submitted to university of Chicago Functional genomic facility for library preparation (TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold, RS-122-2301) and sequencing on Illumina HiSeq2500 platform, with 3 biological replicates for each condition.