GSM2721829: RR263;protein: NHA-Piwi;reporter 1356:Luc-5BoxB-LacZ;OSC;PIWI IP; Drosophila melanogaster; ncRNA-Seq
piRNAs present in PIWI, Aub or Ago3 complexes were isolated using indicated antibodies. Sequencing libraries  were prepared using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Cat No:E7300) according to manufacturer instructions. The synthesized libraries were resolved on agarose gel and fragments of around 120 bp (corresponding to piRNAs) sequenced on Illumina HiSeq platform (EMBL Heidelberg Gene core facility).