GSM3302200: zr7530-CDK12-2; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol reagents. The isolated total RNA was processed for preparing mRNA sequencing library using TruSeq stranded mRNA sample preparation kit (illumina, San Diego, CA) according to manufacturer's instruction. Simply, mRNAs were isolated from 1 ug total RNA by RNA purification bead using polyA capture, and followed by enzyme shearing. RNA libraries were prepared for sequencing using standard Illumina protocols. The protocol consisted of polyA-selected RNA extraction, RNA fragmentation, random hexamer primed reverse transcription and 100nt paired-end sequencing by Illumina HiSeq2500. The libraries were quantified using qPCR according to the qPCR Quantification Protocol Guide and qualified using an Agilent Technologies 2100 Bioanalyzer.