amplicon sequencing of CLVd Chaipayon-1
RNA was extracted from plant leaves, followed by cDNA synthesis. CLVd primers were extended with variable length indices on the 5-end of each primer, so that each sample could be identified with a unique dual index during sample demultiplexing. PCR products were purified with magnetic beads, pooled and ligated with Illumina adapters using the KAPA Hyper Prep PCR-free ligation kit.