16s rRNA target sequencing from soil microcosm  under fipronil F3
For metagenomic library construction, primers for V4 and V5 regions of the 16S ribosomal RNA gene were used for PCR amplification (515F 5'-TGTGNCAGCMGCCGCGGTAA-3' and 926R 5'-barcode-CCCCGYCAATTYMTT-3') (Quince et al. 2011)