GSM2366261: CAY583_S; Saccharomyces cerevisiae; OTHER
Cells obtained from FACS were resuspended in 500 µL of 1.2 M sorbitol, 200 mM Tris-HCl (pH7.5), 20 mM EDTA, 0.1% b-mercaptoethanol. Cells were spheroplasted with Zymolyase and then treated with SDS, Proteinase K and RNase A. DNA was purified by phenol chloroform extraction. Library preparation and sequencing was carried out according to Illumina instructions.