GSM2547135: Pa211_IL11; Homo sapiens; RNA-Seq
Total RNA was isolated from human cardiac fibroblasts with RNeasy column kit (Qiagen). Libraries were constructed with TruSeq Stranded mRNA Library Prep kit (Illumina) using 0.8µg of total RNA. RNA-seq, barcoded libraries were multiplexed and sequenced as paired-end reads on Illumina NextSeq 500 platform using NextSeq High output 150 cycles kit across 4 lanes.