GSM2722433: rod-like1; Brassica napus; miRNA-Seq
Total RNA was extracted using the TRIzol reagent (Invitrogen, CA, USA) according to the manufacturer’s instructions. The quantity and purity were assayed with an Agilent 2100 Bioanalyzer (AgilentTechnologies, Palo Alto, CA) to ensure sample reliability. Each RNA sample was generated from the shoot apexes of 6 different plants. sRNA library construction and deep sequencing were performed as described previously. Briefly, small RNAs with 18–30-nt long small RNAs were purified from 10 µg of total RNA in a 15% polyacrylamide gel by electrophoresis and ligated to 5′and 3′ RNA adaptors. The purified RNAs were transcribed into cDNA, followed by PCR amplification to generate the DNA pool. Finally, 4 libraries were sequenced on an Illumina Genome Analyzer at the Beijing Genomics Institute (BGI, Shenzhen, China).