GSM4654456: hiPS_s2d4_R2; Homo sapiens; RNA-Seq
RNA was isolated with RNeasy mini kit (QIAGEN) according to the manufacturer's instructions. RNA integrity number was analyzed using RNA 6000 Nano Kit (Agilent Technologies) on Bioanalyzer (Agilent Technologies), and RNA was quantified using a NanoDrop 1000 Spectrophotometer (Thermo Fisher Scientific). Sequencing libraries were prepared from 4 µg of total RNA using TruSeq Stranded mRNA Sample Preparation Kit (Illumina). The libraries were quantified by Qubit dsDNA BR Assay Kit (Thermo Fisher Scientific), and analyzed using DNA 1000 Analysis Kit (Agilent Technologies). Each library was diluted to 4 nM and pooled together.