Illumina HiSeq 2500 paired end sequencing; Identification of genes involved in pollen development in wheat
Identification of genes involved in pollen development in wheat
Tisue was harvested from immature stamens or pistils from flowers of wheat between Zadok stages 41-49 Plants were grown under a 16 h day 20/15 temperature no treatment Qiagen RNAEasy kit enzymatic rDNA depletion for reduction of rDNA transcripts within total RNA; standard enzyme RiboZero, randomly primed