GSM3589358: KS_10X_iPSC_KS1_K1-1_scRNA-seq; Homo sapiens; RNA-Seq
Single cell suspension was obtained using Accutase Chromium Single Cell 3' Library & Gel Bead Kit v2 (10x Genomics). Targeted cell recovery for each sample was 5,000 cells. Sufficient cDNA for library construction was achieved using 20 amplification cycles for iPSC libraries and 16 cycles for NPC libraries. Sample indexing was achieved using 11 PCR cycles for iPSC libraries and 5 cycles for NPC libraries.