gDNA long read sequencing of Sinorhizobium meliloti strain CXM1-105, liquid culture
The DNA isolation was performed using a modified phenol-chloroform protocol. The isolated DNA was used for construction of a barcoded DNA library according to the 1D Native barcoding genomic DNA (with EXP-NBD103 and SQK-LSK108) protocol(https://community.nanoporetech.com/protocols/1d-native-barcoding-genomi/v/nbe_9006_v103_revr_21dec2016) provided by the manufacturer. The sequencing was performed in All-Russian research institute for agricultural microbiology using a MinIon sequencer. Albacore (v 2.3.1) was used to basecall the raw fast5 files.