GSM4039714: 1990L_S53; Mus musculus; RNA-Seq
RNA was extracted using an OMNI Bead Ruptor and 2.8 mm ceramic beads (OMNI International) in RLT buffer followed by Qiashredder and RNeasy kit using Qiacube (Qiagen) automated extraction according to manufacturer's specifications. Total RNA was quantified using Quant-iT RNA Assay Kit (Thermo Fisher Scientific). Complementary DNA was prepared using qScript reverse transcription kit (Bio-Rad) and qPCR was performed using Perfecta SYBR mix (Quantabio) and StepOne Plus Real Time PCR system and software (Applied Biosystems). RNA libraries were prepared with QuantSeq 3'mRNA-Seq Library Prep Kit (Lexogen) for the Apollo 324 NGS Library Prep System. Illumina NextSeq 500; 75 bp single-end read.