GSM3462805: RPMI_d6_H3K27ac; Homo sapiens; ChIP-Seq
Purified cells were fixed with 1% formaldehyde (Sigma) at a concentration of approximately 10 million cells/ml. Fixed cell preparations were sonicated using a Diagenode Bioruptor UCD-300 for 3x 10 minutes (30 seconds on; 30 seconds off). 33 µl of chromatin (1 ug) was used for immunoprecipitation. Libraries were prepared using the KAPA hyperprep kit.