Illumina HiSeq 2000 sequencing; Th2 differentiation in a time course experiment and at single generation resolution
Th2 differentiation in a time course experiment and at single generation resolution
Spleens were collected from mice between 6-12 weeks old Untouched naive cells were purified from the spleen with negative selection protocol. Spleens were mashed through a 70nm strainer to obtain single cell suspension Cells were grown in complete IMDM 10% FBS, in presence of IL4 (10ng/ml) Libraries were prepared according to Illumina Paired End sequencing Protocl