GSM3208720: MSMEG_0916_uninduced; Mycolicibacterium smegmatis; ChIP-Seq
Biological triplicate cultures of M. smegmatis containing pDTCF-MSMEG_0916 in 7H9 media, were grown as 50 mL cultures , at 37ºC, 5%CO2, for 4 hours in the presence or absence of 100 ng/ml anhydrotetracycline. DNA libraries were prepared for sequencing using ThruPLEX DNA-seq Kit (Rubicon) standard protocols