GSM1430015: Twist Peconic ChIP-exo (Drosophila embryos) Rep 2; Drosophila melanogaster; ChIP-Seq
embryos were cross-linked in 1.8% formaldehyde. Embryos were then dounced to break down cells and extract nuclei. Chromatin was sonicated by bioruptor to an average size of 300~500 bp. 300 ul soluble chromatin from ~100 mg embryos was used Peconic LLC performed library construction as described by Rhee and Pugh (Cell 2011)