GSM4308373: Endothelial cells from B16F10 melanomas grown on wild type mice 1; Mus musculus; RNA-Seq
Total RNA from tumors or from magnetic cell sorting (MACS®)-isolated cells was isolated using the RNeasy mini-kit (Quiagen, Hilden, Germany). EC (CD31+ cells after pre-depletion of CD11b+ cells) RNA concentration and quality were assessed using the Agilent 2100 Bioanalyzer, and RNA library preparation was done TruSeq stranded total RNA library preparation kit (#15031048) with Ribo Zero Gold treatment.