mouse 16S seq: H2O-1
The V4 region of the bacterial 16S rRNA gene was amplified by triplicate PCR (F515/R806) using barcoded fusion primers. Samples were pooled in sets with a maximum of 96 samples in equal quantities. Paired-end sequencing of the amplicon library was performed on the Illumina MiSeq 300-bp paired-end platform at the University of Minnesota Genomics Center