GSM2264797: 35141_IGF1R_flfl3; Mus musculus; RNA-Seq
Olfactory epithelium was dissected and RNA was isolated using RNEasy Qiagen columns. RNA-seq library preparation and measurement was performed by the Cologne Center for Genomics, Cologne, Germany. Stranded TruSeq RNA-seq library preparation was principally conducted as previously described (Sultan et al., 2012) with 2 μg of extracted RNA and poly-A enrichment. Barcoded libraries were sequenced with 100 bp paired-end reads on an Illumina HiSeq2500.