miRNA CR5, replicate 5
Serum miRNA profile of rhesus monkeys on calorie restriction or control diet was analyzed. For each group 5 samples were processed. Serum was collected, total RNA was isolated using miRNeasy kit (Qiagen). One fourth (5 _L) of the RNA isolated from each plasma sample was used to construct sequencing libraries with the Illumina TruSeq Small RNA Sample Prep Kit (Illumina). The libraries were pooled in equimolar amounts and submitted to sequencing.