GSM3209002: B6_IL4c_sample_0003; Mus musculus; RNA-Seq
RNA was isolated using the RNeasy Mini Kit (QIAgen) based on manufacturer's protocol, with an additional DNase digestion step using the RNase-free DNase set (QIAgen). RNA from each sample was uniquely barcoded and libraries were constructed using the CELSeq2 protocol as described in Hashimshony et al (Genome Biology 2016).