GSM3133019: Veh_H_1; Mus musculus; RNA-Seq
Total RNA from the mouse ventricle of different treated groups was used to perform transcriptome sequencing in the Beijing Annoroad Genomics Co. Ltd , five biological replicates was performed in each group. Total RNA of all sample were isolated with Mini RNeasy Mini kit (QIAGEN). Agilent 2100 RNA Nano 6000 Assay Kit (Agilent Technologies, CA, USA) were used to detected the integrity of the RNA, and then mRNA were enriched with beads with Oligo (dT). Purified mRNA was degraded by Fragmentation Buffer to perform short mRNA fragment. Random primer and relevant buffer were used to synthesis cDNA, and then cDNA was purified with QIAQuick PCR kit and EB buffer. Purified cDNA was modified and amplified by PCR to construct cDNA libraries . Each cDNA library was sequenced on an Illumina HiSeq2000 instrument. Bioinformatically, reads with no more than 30nt length are considered from mature miRNAs, and were counted and mapped to the genome using Bowtie alignment tool. RNA libraries were prepared for sequencing using standard Illumina protocols