GSM2545750: WT root +R.i replicate 1; Petunia x hybrida; RNA-Seq
Samples of about 100mg were frozen in liquid nitrogen, stored at -80°C and lyophilised before extraction. Total RNA was extracted from lyophilized roots using the guanidinium thiocyanate-phenol-chloroform method and DNase treated on column (DNA, RNA and protein purification, Macherey-Nagel, Switzerland) Preparation of 12 libraries and 2 x 100bp pair-end Illumina HiSeq mRNA sequencing (RNA-Seq) was performed by Beckman Coulter Genomics (Danvers, MA, USA)