GSM3463562: KW734TEK100; Mus musculus; RNA-Seq
RNA extracted using mirVana miRNA Isolation Kit following the manufacturer's protocol Library construction was performed by Novogene. rRNA was removed using the Riob-Zero kit that leaves mRNA. mRNA is randomly fragmented and cDNA synthesized using mRNA template and random hexamer primers and custom second-strand synthesis buffer (Illumina), dNTPs, RNase H and DNA polymerase were added to initiated second strand synthesis. After a series of terminal repair, A ligation and sequencing adaptor ligation, the double stranded DNA library was completed through size selection and PCR enrichment