GSM2720098: P0.2; Mus musculus; RNA-Seq
Fresh frozen mouse retinas were lysed with a mortar and pestle in Trizol Reagent, and total RNA was isolated per manufacturer’s protocol (Invitrogen, Carlsbad, CA). RNA quality and quantity were assessed with RNA 6000 Nano Kit (Agilent, Santa Clara, CA). Directional RNA-seq library construction was performed as previously described (Brooks et al., 2012). Briefly, 100ng (embryonic days 11 and 12) or 1ug of total RNA was used for library construction using Illumina mRNA Library Prep Kit with modifications for directionality.