Total RNA-seq of 93T449 WDLPS cell line
Total RNA was extracted using the RNeasy Mini Kit (Qiagen, Hilden, Germany) and checked for integrity by microcapillary electrophoresis on the 2100 Bioanalyzer instrument (Agilent Technologies, Santa Clara, CA, USA). Total RNA-seq libraries were prepared using the TruSeq Stranded Total RNA Library Prep Kit (Illumina, San Diego, CA, USA), and sequenced on the HiSeq 2000 platform in a 2 × 101-cycle run, at the IGA Technology Services facility (Udine, Italy), with about 70M reads/sample.