GSM3669436: col_T0_2, sRNA; Arabidopsis thaliana; miRNA-Seq
Small RNAs were extracted using mirVana™ miRNA Isolation Kit (Ambion AM 1560, part IV/A. Isolation of Small RNAs from Total RNA Samples). Small RNA libraries were construct from 50ng of miRNA using Ion Total RNA-Seq Kit v2 (Ion Torrent, Life Technologies) according to manufacturer instruction. Libraries were then sequenced on Ion PI™ Template OT2 200 Kit v3 on ION PI v2 chip. Adapter were removed