GSM2455098: Tia1 iCLIP LPS-activated B cells Replicate 2; Mus musculus; OTHER
Cytoplasmic extracts were isolated from 2-3 x 10E7 cells after irradiation with 300mJ/cm2 UV-C light in a Stratalinker. RNA-protein complexes were purified using a 2 µg of goat anti-Tial1 (C-18) antibody (sc-1749) after partial RNA digestion with RNAse I (0.3 U/ml) See König et al, 2010 Nature Structural and Molecular Biology, 17, 909-915. Individual-nucleotide resolution UV crosslinking and immunoprecipitation (iCLIP). Samples were multiplexed based on a 7 bases barcode (annotated under sample description)