GSM2977599: Isotype [Single Cell RNA-seq]; Mus musculus; RNA-Seq
5,000 cells from isotype or 7C6-mIgG2a groups were targeted for the 10X Genomics 3' V2 single cell assay (10X Genomics). Reverse transcription, cDNA amplification and library preparation were all performed according to the manufacturer's instructions. Libraries were sequenced using an MiSeq on rapid-run mode, which yielded >25,000 reads per cell.