24h heat treatment of XK in non-heading Chinese cabbage
Messenger RNAs (mRNAs) from the total RNA were isolated using Oligo (dT) and were randomly cleaved into short fragments. Then the first strand cDNAs were synthesized with random hexamer primers and followed by second strand cDNAs synthesis using DNA polymeraseⅠ(New England BioLabs) and RNase H (Invitrogen). After end repair, adaptor ligation, and index codes adding for each sample, PCR amplification was performed. The qualified cDNA library was carried out by an Illumina HiSeq 2000 platform.