Amplicon sequencing for the mutation in iscR of Escherichia population isolated at day 3  from old mouse A3
The sequencing was performed at the genomics facility at Instituto Gulbenkian de Ciencia (Portugal). Specific primers for iscR (iscR_Fw and iscR_Rv) were used for library construction. Briefly, a sample of mice feces was plated on LB agar and allowed to grow till ~1000 isolated colonies were visible. These colonies were then pooled and DNA was extracted. A PCR was then performed using the primers iscR_Fw and iscR_Rv and the product was used for amplicon sequencing.