GSM4258382: CT1-replicate2; Mus musculus; RNA-Seq
Anterior portion of the whole eye removed, neural retina peeled off, and posterior portion containing retinal pigment epithelium, choroid, and sclera were placed in RNAlater (Qiagen, 76104) for brief sonication. Isolated retinal pigment epithelium cells were placed in TRIzol (Ambion, 15596018) for total RNA isolation. RNA library was constructed using QuantSeq™ 3'mRNA-Seq Kit (Lexogen, 015.24) per the manufacturer's protocol.