Sample from treatment of ambient temperature and drought seedlings in leaves
Poly A+ mRNA was isolated from total RNA using the RNA Purification Beads (Illumina) and then broken into fragments as the templates for the synthesis of first- and second-strand cDNA. Double-stranded cDNA was isolated using Ampure XP beads (Beckman Coulter, Beijing, China) and short fragments were purified with the QubitTM dsDNA HS Assay kit (Invitrogen, US). After connected with sequencing adapters, the libraries were quantified by Qubit 2.0 Fluorometer (Invitrogen) and validated by Agilent 2100 bioanalyzer (Agilent, Beijing,China).