RNA-seq of bassica napus
The resistant (R) and sensitive (S) lines, both untreated and aging treated for 15 d, were pooled at an equal quantity to form four libraries, i.e., R0, R15, S0 and S15, which were used for the transcriptome library construction. The total RNA was extracted from 50~70 mg of frozen seeds using the Plant RNA Mini Kit (TIANGEN, China). Nanodrop2000 was used to evaluate the quality and purity of the extracted RNA. The RNA Integrity Number (RIN) was assessed by Agilent 2100 (Agilent). The sequencing library was generated using the TruSeqTM RNA sample Prep Kit (Illumina). The libraries were sequenced on an Illumina HiSeq 4000 platform