GSM2546320: SAM24315070 KO pvm 3; Mus musculus; RNA-Seq
RNA was isolated using RNeasy Kit (Qiagen). The quantity and quality of total RNA extracted were determined prior to the processing by RNA-seq. The concentration of total RNA samples was determined using NanoDrop 8000 (Thermo Scientific). The integrity of RNA samples was determined using 2100 Bioanalyzer (Agilent Technologies). < 0.5 mg of total RNA was used as an input material for library preparation using TruSeq RNA Sample Preparation Kit v2 (Illumina). Size of the libraries was confirmed using 2200 TapeStation and High Sensitivity D1K screen tape (Agilent Technologies) and their concentration was determined by qPCR based method using Library quantification kit (KAPA). The libraries were multiplexed and then sequenced on Illumina HiSeq2500 (Illumina) to generate 30M of single end 50 base pair reads.