GSM2545384: En1Cre;b-cat mut4; Mus musculus; RNA-Seq
At E13.5, En1Cre;b-cateninfl/fl mutant and En1Cre;b-cateninfl/+ control cranial mesenchyme was isolated by manual dissection. An incision was made around the circumference of the neurocranium, and the tissue covering the brain was manually disassociated. The supraorbital cranial mesenchyme isolated consists of the neural crest and mesoderm derived cranial mesenchyme plus the ectoderm. Illumina TruSeq Stranded Total RNA kit-with Ribo Zero Gold