Antibiotic (carbadox) effects on the swine gut microbiota
DNA and RNA samples were extracted from the same fecal samples from piglets n = 12 from two sows that were divided into two treatment groups non-amended feed or 50 g carbadox / ton feed as a previous study {Looft:2014bj}. Prior to nucleic acid extraction, an artificial RNA spike-in name the vector used, how linearized, purification, size was added to the fecal material as described previously {Gifford:2011eo} to normalize transcript counts by g fecal material. RNA and DNA were extracted from the same fecal material using the PowerMag Microbiome RNA/DNA isolation kit following the manufacturers instructions. Bacterial community DNA was sequenced on a Roche GS-FLX instrument with Titanium chemistry 454 Life Sciences