GSM3302186: mesenchymal type #1; Homo sapiens; miRNA-Seq
Total RNA was isolated from samples using mirVana miRNA isolation kit (Invitrogen). NEXTflexTM Small RNA-Seq Kit v3 (Bioo Scientic #5132-05) was used with 1 ug of total RNA for the construction of small RNA sequencing libraries. small RNA libraries were prepared for sequencing using standard Illumina protocols.