GSM4654212: Streaming stage replicate 3 [HiSeq]; Dictyostelium discoideum AX4; RNA-Seq
Cells were dissociated into single cells from different stages by physical pipetting with PBS + 0.04% BSA. The cell numbers were counted using a microscope. Cell from each sample were subject to Chromium Single Cell Gene Expression kit V3 (10x Genomics) for library construction according to the manufacturer's instructions.