GSM2877468: malignant nodule 138T; Homo sapiens; Bisulfite-Seq
DNA from fresh frozen samples was isolated by using the phenol/chloroform extraction approach. For DNA isolation from FFPE samples, QIAamp DNA FFPE Tissue Kit was used (Qiagen Inc., Valencia, CA) The RRBS procedure was previously described (Hahn et. al., 2015; PMID: 25421665). DNA sequencing was performed on an HiSeq Illumina instrument and aligned to the human genome at the City of Hope Integrative Genomics Core.