GSM4655043: ELa3-1_WT: WT zebrafish embryos rep 1; Danio rerio; RNA-Seq
Embryo pools of roughly 50 embryos were dropped into trizol, homogenized, and RNA was isolated through phenol-chloroform extraction and ethanol precipitation. RNA was quantified using a Nanodrop 2500 and assessed for quality using Bioanalyzer 2100 RNA chips (Agilent). Samples with RIN numbers above 9 were selected for further processing into libraries. Libraries were constructed using the NEBNext Ultra RNA Library preparation kit (E7530S) with poly(A) mRNA magnetic isolation module according to manufacturer instructions