GSM2104460: 10ng rep2; Homo sapiens; MeDIP-Seq
The genomic DNA of the collected cells were extracted using Thermofisher PureLink® Genomic DNA Kit and eluted in EB buffer, prior to being sheared using a Covaris M220 shearing instrument to a target size of 150 bp.The sheared product was size selected and purified with the MinElute Gel Extraction kit (Qiagen) to 100-250 bp and 250-400 bp. The concentration of the purified fragments was measured through Qubit prior to pooling with a ratio of 10:1 of 100-250 bp:250-400 bp DNA product to make test samples with the following DNA amounts: 100 ng, 10 ng, 5 ng and 1 ng. antibody: MeDIP kit manufacturer Diagenode; Kit Catalog #: C02010021; the lot number of antibodies include: Lot GF-003, LotD006 The libraries were prepared using the Kapa Hyper Prep Kit