GSM2545347: CNS_RNA-seq_26C; Mus musculus; RNA-Seq
Mice were anethetized with ketamine and xylazine then perfused with sterile phosphate buffered saline (PBS).  The cerebellum and spinal cords were dissected and flash frozen in liquid nitrogen.  RNA was extracted using Tri reagent, then further purified using GeneJet RNA purification kit and DNase digestion. The strand-specific RNAseq libraries were prepared with Illumina's 'TruSeq Stranded RNA Sample Prep kit'. Adaptors must be remove. Sequence of adaptor for read1: AGATCGGAAGAGCACACGTCTGAACTCCAGTCACNNNNNNATCTCGTATGCCGTCTTCTGCTTG  (NNNNNN= 6 nt index). Sequence of adaptor for read2: AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCAT