GSM2264269: Tick17; Ixodes ricinus; RNA-Seq
Total RNA was extracted using TRIzol (Invitrogen), according to the manufacturer's protocol, and further purified using the Rneasy Mini kit (Qiagen). Ribosomal RNA was depleted using Terminator Exonuclease (Epicentre). Double stranded cDNA synthesis using Roche cDNA Synthesis System.  Illumina library construction using NexteraXT kit