GSM4041483: Pemphigus vulgaris IP07 Sample52; synthetic construct; OTHER
The barcoded DNA template was PCR-amplified as follows: 95°C for 2 min; followed by 4 cycles of 10 s at 95°C, 10 s at 60°C, and 1 min at 68°C; 25 cycles of 10 s at 60°C and 2 s 68°C of 1 min; and a final elongation step for 5 min at 68°C. The amplified DNA was purified by using Agencourt AMPure XP kit (Beckman) and the quality of the DNA library was assessed by Bioanalyzer (Agilent, USA), as recommended by the manufacturer. The amplified 174-bp products, including the Illumina adapter and index sequence, were analyzed by the MiSeq system (Illumina) with dual-indexed sequencing in a paired-end flow cell, to read 75 bp, including the amplified 38 bp of barcode DNA HaloTag barcode-seq