Serratia Y1-0h
Briefly, the 3 and 7 RNA adapters were ligated to the corresponding ends of small RNAs. Following adapter ligation, the ligated RNA fragments were reverse transcribed using M-MLV reverse transcriptase . The resulting cDNA products were PCR amplified witwo primers that were complementary to the ends of the adapter sequences. The PCR amplicons were separated by size in 6% polyacrylamide gel for sRNA (20-30 nt) enrichment and sequenced on Illummina HiSeq 2500 sequencing system.