Sample 17.5 km downstream from the Indian WWTP (R1)
The DNA extraction was performed by using the MoBio PowerSoilTM DNA Isolation Kit (Solona Beach, CA). The amplification was done using REPLI-g mini kit (Qiagen). Sequencing was done at GATC Biotech (Konstanz, Germany) using a Roche Genome Sequencer FLX Titanium. Barcode-based multiplexing where used to sequence the eight samples simultaneously.