RNA-seq of post-larval Spirinchus thaleichthys: whole fish: Sample Longfin smelt_14degrees_60G
"Whole fish were homogenized using a TissueLyser (Qiagen) in Buffer RLT Plus (RNeasy Plus Mini Kit; Qiagen) and 350 ?l of the homogenate was used for the RNA extraction on a Qiacube (Qiagen) following manufacturer’s protocols. RNA quality was assessed using a Bioanalyzer (Agilent) and concentration was measured using a Qubit Fluorometer (Life Technologies). 1.5 ?g of total RNA was used to prepare 10 separate Poly-A isolated, strand-specific cDNA libraries using Directional PrepX RNA Library Prep kits (Wafergen Biosystems) barcoded with a unique adapter prior to sequencing. All libraries for longfin smelt (n=10) were pooled and sequenced on an Illumina HiSeq 2500 over two lanes. Sequencing was conducted to produce 100 base pair paired-end reads for each individual. "