Genome-wide SNP detection in Parus major
Blood from ten wild caught hand-reared male great tits (Parus major) from ten different broods was used as the starting material for DNA isolation. In order to reduce complexity, we generated two reduced representation libraries (RRLs). The sample was size-fractionated on a 1% low melting point agarose gel (SeaPlaque). The size fractions of 3000 to 3500 bp (Gt3000) and 3500 to 4000 bp (Gt3500) were purified from the gel. For library preparation the Genomic DNA Sample Prep Kit (Illumina) was used according to the manufacturers' instructions, with the exception of phosphorylation of the sample. Randomly sheared, adapter ligated, fragments in the size-range of 170-250bp were used as the starting material for sequencing.