RNA-Seq of PA-KP static co-culture
The first-strand cDNA was synthesized from fragmented RNA by using random hexamers. The second-strand cDNA was synthesized using dUTP instead of dTTP followed by purification by using AMPure XP beads. Uridine digestion was performed by using USER Enzyme. Paired-end libraries were prepared from purified cDNA and PCR primers were removed using AMPure XP beads.