F113  Fe added exponential phase replicate 1
RNA was extracted from cultures grown in SA medium supplemented with Iron to exponential (OD600 0.6) and stationary phase (OD600 1.2). Three independent cultures per strain and condition were mixed prior to RNA extraction.  RNA isolation was performed using Trizol (Invitrogen) followed by an isopropanol precipitation. The obtained RNA was treated with DNase (RQ1) and finally was purified using the RNAclean and Concentratortm-5 column (Zymo Research). rRNA depletion, library construction and sequencing was custom performed by Sistemas Gen micos (Paterna, Valencia). Two libraries per sample were prepared and sequencing was carried out using Illumina MiSeq paired end, 2x100 bp.