WGS of Gallus gallus: North American Araucana
Genomic DNA adjusted to a concentration of 50 ng/L in 10 mM Tris-CL buffer pH 8.5, measured by optical density using a NanoDrop 1000 Spectrophotometer (Thermo Scientific, Wilmington, USA). One hundred fifty ng of DNA was used for 2x100 bp paired-end sequencing, on an Illumina HiSeq 2000 at the Roslin Institute at The University of Edinburgh (Easter Bush, Midlothian, Scotland, UK). The following fragments were gel size selected: 440-489 bp.