Single-cell WES Mlh1+/+ thymic T cell BioRep2
Naive thymic T cells were enriched from thymus. Single T cells were captured, lysed and whole genome amplified using IFC 5-10 _m capture plate in Fluidigm C1 system. Agilent SureSelectXT Mouse All Exon 49.6Mb capture was used for exome enrichment and library preparation from single-cell genomic DNA samples. Sequencing libraries were sequenced in Illumina NextSeq 500 with mid output reagents.