MG1655 Experimental evolution of a thermophile from a mesophilic ancestor starting strain
Genomes were sequenced using the Solexa/Illumina sequencing platform. Briefly, genomic DNA preparations were made using DNEasy kit (Qiagen). Genome libraries of each strain were generated using the Genomic DNA sample prep kit (Illumina) as described by the manufacturers directions. Sequencing was performed in a 36 cycle single end run (Core facility, Oregon state university, OR).