Tissue Specific De Novo Transcriptome Sequencing for the Identification of Candidate Genes linked with Starch Biosynthesis Pathway in Wheat under Terminal Heat Stress
he RNA was ribosomal depleted and was chemically fragmented and converted to cDNA. The Illumina adapter ligation was performed using Stranded Protocol and Sequenced On Illumina HiSeq 4000 with 2 X 100 paired end sequencing.