adult male thymocytes
Total RNA from mouse thymocytes was isolated using TRIzol (Invitrogen, 15596026). Integrity of RNA was assessed using the Agilent 2100 Bioanalyzer (Samples with RIN > 8 used for manipulation). Messenger RNA were enriched with Oligo-dT beads (NEB) and then used for RNA library construction using Next Ultra RNA Library Prep Kit (NEB) according to the manuals. The cDNA libraries were pooled and sequenced with Hiseq X Ten Sequencing Platforms (Illumina).