Subgingival plaque of tooth 42 from patient 04 (deep probing site)
Metagenomic DNA extracted from subgingival plaque was used as template for PCR amplification using primers targeted to the V4 region of 16S bacterial rDNA as described by Kozich et al., 2013, Appl Environ Microbiol. 2013 Sep;79(17):5112–5120