D.lacteum:finger
RNA was prepared from growing cells and two multicellular developmental stages: aggregates and tipped aggregates/fingers (biological duplicates except for D. lacteum). Truseq small RNA Sample Preparation kit (product # RS- 200-0012, Illumina) was used to prepare sequencing libraries from 1 g total RNA. The library preparation was performed according to the manufacturers protocol (#15004197 rev G) where cDNA representing 18 nt  70 nt RNA were isolated. Single read 50 bp sequencing was performed using v4 sequencing chemistry on an Illumina HiSeq2500. To reduce influence of degradation products, only full length 50 bp reads were mapped with bowtie allowing for 1 mismatch