dry colony, biological replicate #2
Total RNAs were extracted from at least 5 gametophores from each colony using Qiagen Plant RNeasy extraction kit (Qiagen) and RNase free DNase I (Qiagen, Germany). Three different biological replicates were prepared. The integrity and concentration of RNA was determined using Bioanalyzer and Qubit RNA BR assay kit (Life Technologies, USA). To construct the sequencing library, 1.5 g of total RNA of each sample was used as input for the TruSeq RNA sample prep kit v2 (Illumina, USA).