Whole Exome Sequencing of  Hereditary Leiomyomatosis Associated Renal Cell Carcinoma
Eight-mm-thick representative sections of FFPE tumor and marched normal tissues/blood sample were collected. Tumor sections were reviewed by a pathologist to ensure tumor sections with >70% tumors and < 10% necrosis. High-quality genomic DNA was extraction by using GeneRead DNA FFPE Kit (180134, QIAGEN, Duesseldorf, GER) according to the manufacturer. Exome capture was performed using Agilent SureSelect Human All ExonV6 kit (Technologies, CA, USA) according to the manufacturers instructions. This was followed by paired-end sequencing using Illumina Hiseq platform (Illumine Inc, CA, USA)