Transcriptome sequencing of the scleroglucan producer Sclerotium rolfsii
Total RNA extracted from 37 h old cultures of S. rolfsii grown in EPSmin17 and EPSmax13 medium were pooled in a 1:1 ratio to guarantee equal predominance of both RNA populations and reversed transcribed into cDNA. The mixed cDNA sample was sequenced by 454 Life Sciences™ in a triplicate sequencing run and resulted in 356,098 single reads composed of 3.68 million bases.