16S rDNA library from Nahant Canoe Cove: 1 um fraction
V4 variable region of the 16S rDNA from the 1 um fraction of a serially filtered water (63um, 5um, 1um, and 0.2 um). V4 amplified with U515 and E786R with a two step PCR method, and identified in a multiplexed sampled by a sample spcecific barcode