Fungal diversity analysis: soil sample
Gemonic DNAs were extracted from the soil samples. Primers ITS3 and ITS4 were used to amplify the ITS2 region of the fungal gemonic DNAs. A bar code sequence was added in each forward primer. PCR was conducted in triplicate, and the products were pooled for each subsample. A composite DNA sample for sequencing was created by combining equimolar ratios of amplification products from each sample.