RNA-Seq of Navid cv. grains at 28 day post anthesis
Grain sampling was done at 28 day post anthesis (DPA). Total RNA of ten grains of each spike was isolated by TRIzol Reagent (Invitrogen) and the extracted RNAs of 10 spikes were pooled to obtain the final RNA sample for sequencing. mRNA capturing from total RNA was done using PolyA approach and RNA sequencing was conducted as paired-end with 150nt length using Illumina HiSeq 2500.