RNA-Seq data from Cystoisospora suis six-days after infection merozoites
Cystoisospora suis merozoites were maintained in IPEC-J2 cells as described in (Worliczek et al., 2013). Free merozoites (8×106) were harvested by collecting supernatant 6 days post infection, and purified on a Percoll® density gradient. Purified merozoites were then filtered through Partec CellTrics® disposable filters (50 μm), washed twice with PBS and pelleted by centrifugation at 1000 x g for 10 min. Total RNA was extracted from purified pelleted merozoites using a QIAamp RNA blood mini kit (Qiagen, Germany) according to the manufacturer’s instructions and was quantified using a spectrophotometer (NanoDrop 2000, Thermo Fisher Scientific, USA).