Marine eDNA rom 18S V3-V4
The eukaryotic-specific primers were Uni18SF: 5-AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al., 2013) modified to include IlluminaTM overhang adaptors.