RNA-Seq of Arabidopsis ise4 mutants
Samples were collected from siliques of heterozygous mutant/wild-type parents.  Only seeds with mid-torpedo stage development were included.  At least 100 seeds from at least 10 individual parent plants were included in each replicate.  Tissue was snap frozen upon collection in RNAlater.  Total RNA was extracted with Qiagen kits, rRNA depleted, and then used to synthesize libraries for Ion Torrent sequencing.