RNA-seq of 3-dpf perithecia of Neurospora crassa (FGSC2489 x FGSC4200)
The total RNA  was extracted and purified with the RNAprep Pure Plant Kit (Tiangen Biotech, Beijing, China). Poly(A)+ mRNA was enriched from total RNA using immobilized oligo (dT). RNA-seq libraries were prepared using the NEBNext? Ultra? Directional RNA Library Prep Kit (NEB, USA) as described by the manufacturer, and sequenced by Illumina HiSeq? 2500 with a 2¡Á150 bp paired-end read mode.