16S rRNA amplicon of epinecrotic bacterial community of pigs
For the analysis of metagenomic taxonomic inventory, the hypervariable V3-region of the 16S rRNA gene was amplified via the Bartram approach using multiplexing (Bartram et al., 2011). After the removal of adapter dimers via MetaPhorŽ agarose (Lonza, Basel, Switzerland) and subsequent gel purification via the NucleoSpinŽ Gel and PCR Clean-up Kit (Macherey-Nagel, Merck, Darmstadt, Germany) the samples  were sequenced on a HiSeq 2500 Ultra-High-Throughput Sequencing System (Illumina, San Diego, CA, USA) technology