RNA-Seq of Symbiodinium microadriaticum: 16C/36C/Dark_Stress/Control treatments
3 independent S. microadriaticum cultures were grown in 200 ml of F2 media (Sigma-Aldrich) for 2 weeks in non-treated Nunclon _ TripleFlasks (Thermo Scientific) at 26 ĄC. The cultures were illuminated at a constant irradiance of 250 _mol photons m-2ás-1 on a 12h:12h day:night light-cycle (daytime of 6 amĐ6 pm). Prior to the day where cells were collected, the three bottles were placed in an incubator programmed to ramp up to 36 ĄC at 8 am ("36C": extreme heat stress for 4 hours). Paired-end reads for Illumina sequencing were generated from oligo-dT selected total RNA using the NEBNext Ultra Directional RNA Prep Kit.