WGS of Mycobacterium bovis BCG-1 (Russia): clinical isolate
The DNA samples were purified by RNase A (Thermo Scientific #EN0531) and sonicated using Bioruptor UCD-200 system (Diagenode, Denville, NJ, USA) to prepare paired-end (PE) libraries by following NEB Library Preparation Protocol, as seen in the product manual for the NEBNext Ultra II DNA Library Preparation Kit for Illumina (NEB #E7645).