Amplicon sequencing on Illumina MiSeq of Aegilops geniculata: accession AE 925
PCR primers of 20-26 bp were designed in exons encompassing intronic regions of about 400 bp for 48 low-copy nuclear loci, with an annealing temperature arounf 60 C. PCRs of all nuclear loci were carried simultaneously on 48.48 IFC plates following Fluidigm protocol. Amplicons were then pooled and barcoded by sample for paired-end (2x300 bp) Illumina MiSeq.