RNA-seq of Limulus polyphemus: central nervous system
Animal was sacrificed at 18:00 and it's entire central nervous system (protocerebrum, subesophageal ganglia and ventral nerve cord) was removed and frozen on dry ice. The RNA was extracted using Ambion TRIzol and purified using Qiagen's Rneasy Mini kit. Libraries were prepared using Illumina's TruSeq RNA Sample Prep LT and loaded on the sequencer at ~12pM.