LD04D3
Extracted DNA quantified via Quant-iT PicoGreen dsDNA Assay, concentration normalized, library prepped via Nextera XT DNA Library preperation kit (Illumina), libraries pooled with equal volumes, insert sizes and concentration determined via Agilent Bioanalyzer DNA 1000 kit, and libraries sequenced on HiSeq 2500 2x101. De-multiplexing and FAM and FASTQ file generation via Picard suite.