16S rRNA seq: rabbit carcasses
Amplification of the V1-3 region of the bacterial 16S rRNA gene was carried out using universal barcoded-fusion-primers: Gray28 FW (5'-TTTGATCNTGGCTCAG-3') and Gray519 RV (5'-GTNTTACNGCGGCKGCTG-3'). HotStarTaq Master Mix (QIAGEN) was used to prepare a master reaction of 50 ml according to manufacturers instructions.