RNA-seq (454) of Pratylenchus penetrans: gland cells
Total RNA from microaspirated gland cells was isolated with Arcturus PicoPure kit, then amplified with Epicentre RiboMultiplier Sense-RNA Amplification kit.   Amplified RNA was purified on 5% PA gel and fractions larger than 300 nt were eluted O/N, precipitated, quantified and used for library construction.