ecop-nanopore-1
Dneasy PowerSoil Kit used for isolate DNA, Oxford Nanopore MinION sequencing using the 1D approach following default Oxford Nanopore protocols for library preparation. Sequencing of E. coprostanoligenes on the MinIon was performed with a R9 flow cell. rior to assembly, the Illumina reads were trimmed with Trimmomatic 0.36. Spades 3.9.0 was used to perform a hybrid assembly with the Illumina and Oxford Nanopore MinIon reads using the --nanopore option.