Fungal ITS Amplicon of BSHP.26
Total DNA was extracted from 0,2 g of rock sample using MOBIO Power Soil DNA Extraction kit (MOBIO Laboratories, Carlsbad, CA, USA). ITS1 rRNA region was amplified using ITS1F/ITS2 primers which were developed for shorter read length (Smith and Peay, 2014). PCR reactions were carried out with a total volume of 25 μl, containing 5 ng of DNA.