RNA-Seq of Homo sapiens: Old CD8 Effector Memory
T cells were isolated by FACS, followed by RNA extraction with RNeasy Plus Micro Kit (Qiagen), RNA quality was conformed with a 2100 Bioanalyzer (Agilent Technologies). Libraries were prepared using Ovation Human FFPE RNASeq Library Systems (NuGEN, San Carlos, CA), quantified with a KAPA Library Quantification Kit (Kapa Biosystems, Wilmington, MA) and sequenced on an Illumina NextSeq 500 at the Stanford Functional Genomics Facility.