Illumina sequencing of biovar3 Psa M228
Genomic DNA was extracted from bacteria using Mo Bio Microbial DNA Isolation Kit (GeneWorks NZ, Ltd) following the manufacturers instructions. The sequencing library was prepared using the Illumina TruSeq DNA Sample Preparation v2 kit following the standard low throughput protocols. Paired-end sequencing was performed to 100 bp on a HiSeq2000 system and demultiplexed using the Illumina Casava application, v1.8.2.