UCE target enrichment of Aegolius-acadicus-acadicus_UAMX2980: muscle
We prepared dual-indexed DNA libraries for each sample using methods described in Glenn et al. (2017). After library preparation, we quantified each library using a Qubit fluorimeter (Invitrogen, Inc.), and we combined eight libraries into equimolar pools of 500 ng each (62.5 ng/library). We enriched the pool of 8 samples for 5,060 UCE loci using the Tetrapods-UCE-5Kv1 kit from MYcroarray following version 1.5 of the UCE enrichment protocol and version 2.4 of the post-enrichment amplification protocol (ultraconserved.org) with HiFi HotStart polymerase (Kapa Biosystems) and 14 cycles of post-enrichment PCR. We then quantified the fragment size distribution of the enriched pool on a Bioanalyzer (Agilent, Inc.) and qPCR quantified the enriched pool using a commercial kit (Kapa Biosystems).