We harvested leaves from ~3 weeks old plants grown under long day conditions (16 hrs. light and 8 hrs.) at 16ĄC. We extracted DNA in 96 plates with the NucleoMag¨ 96 Plant (Macherey-Nagel) kit according to the manufacturerŐs instructions. We prepared libraries using the Illumina NexteraŞ Kit. Standard Nextera library construction was modified to reduce volume in the protocol. Briefly, tagmentation reaction was set up to 2.5 ľl final volume with 2.5 ng of input DNA. For PCR amplification and multiplexing we used Nextera Index Kit (Illumina) indexes. Size selection and PCR clean-up were performed with Agencourt AMPure XP Beads (Beckman Coulter). After PCR enrichment, libraries were validated with Fragment AnalyzerŞ Automated CE System (Advanced Analytical) and pooled in equimolar concentration for 96X-multiplex. Libraries were sequenced on Illumina HiSeqŞ 2500 using manufacturerŐs standard cluster generation and sequencing protocols in 125 bp paired-end (PE) mode.