2014_Plot_17_margin_withAMFungi_highBiochar
DNA was extracted from plant roots and was amplified using forward primer LROR (Bunyard et al 1994) and barcoded reverse primer FLR2 (Trouvelot et al 1999) to amplify a roughly 850bp region covering portions of the variable D1 and D2 regions of the nuclear large subunit (LSU; 28S) rRNA gene, targeting arbuscular mycorrhizal fungi. PCR products were paired-end sequenced (Illumina MiSeq; 300 bp for each read) to create non-overlapping paired reads.