RNA-seq of brown planthopper
The cDNA library was prepared using a SMARTerTM PCR cDNA Synthesis Kit (Clontech, Mountain View, CA, USA) and an Advantage 2 PCR Kit (Clontech). After the end-repair and ligation of adaptors, cDNA products were amplified by PCR and purified using the QIAquick PCR Purification Kit (Qiagen,Hilden, Germany) to create cDNA libraries. The cDNA libraries were sequenced on an Illumina HiSeq 2000 sequencing platform at BGI-Shenzhen