NC68A_LWA_16S
eDNA was extracted from all sediment samples, in duplicate, using the PowerSoil DNA Isolation Kit according to manufacturers instructions. The V4 region of the small subunit ribosomal RNA genes (16S rRNA) were PCR amplified from genomic DNA using barcoded bacterial/archaeal gene primers 515F (5- GTGCCAGCMGCCGCGGTAA-3) and 806R (5- GGACTACHVGGGTWTCTAAT-3) with adaptors CS1 (5- ACACTGACGACATGGTTCTACA-3) and CS2 5'-TACGGTAGCAGAGACTTGGTCT. Index barcodes were unique to each sample.