2b-RADseq of Bouteloua gracilis: aboveground tissue
DNA was digested using the AlfI IIB enzyme (Fisher Scientific) followed by adapter ligation. We ligated specific Illumina adapters to target only 1/256 of the AlfI enzyme cut site fragments. In other words, instead of targeting fragments ending in NN, we targeted only fragments ending in GG for sequencing. Pooled samples were divided  into six lanes followed by an Omega Bio-tek Mag-Bind Total Pure NGS magnet cleanup and concentration step prior to sequencing.