PE125 seq of Pyropia yezoensis: wild type
Qualified DNA was randomly sheared into numerous small segments using a Focused-ultrasonicator (Covaris, USA). Fragmented DNA was end repaired using T4 DNA polymerase and an A base was added to the 3 ends before DNA adapter (Illumina, USA) with a single T base overhang at the 3 end was ligated. After the agarose gel electrophoresis, the suitable fragments of approximately 500 bp were selected, excised and purified. The adaptor modified DNA fragments were enriched via PCR amplification using Illumina paired-end PCR primers (Illumina, USA).