Rna-seq of mus musculus: adult male spleen
Total RNA was isolated following RNAeasy Kit instructions which include on-column DNAse treatment and sent to Expression Analysis (Research Triangle Park, North Carolina (www.ExpressionAnalysis.com)) for sequencing by the paired-end method.  RNA quality was assessed by electrophoresis and bioanalyzer prior to RNA sequencing. An input of 100 ng of total RNA was used to construct cDNA libraries (TruSeq Stranded mRNA Sample Prep Kit, Illumina, #RS-122-2103) following the manufacturers instructions.