D2 SOLiD DNA-Seq
The MM_DBa BAC library was generated at Clemson University Genome Institute in 2002 using a single male D2 strain mouse from the Jackson Laboratory where their Genetic Stability Program uses a cryopreservation approach to effectively limit genetic drift and ensure strain stability. We identified a minimal contig of 27 overlapping MM_DBa BACs in the library screen using the standard Roche DIG chemiluminescent protocol for probing DNA library filters: clones 75C22, 329K2, 163C23, 109M5, 438J2, 138A14, 372P6, 264L1, 69J19, 220P1, 196G24, 250H1, 17F16, 374A8, 271K8, 467L22, 405D20, 431P3, 359G4, 259B4, 246D12, 248E11, 270C10, 440I3, 98C12, 41K6, and 306F23.