Single-cell WES Mlh1-/- thymic T cell BULK DNA control BioRep1 TechRep1
Naive thymic T cells were enriched from thymus. Suspension of thymic T cells were treated with same reagents as single-cell genomic DNA samples (lysis and whole-genome amplification) to create bulk DNA samples. Agilent SureSelectXT Mouse All Exon 49.6Mb capture was used for exome enrichment and library preparation from bulk DNA samples. Sequencing libraries were sequenced in Illumina NextSeq 500 with mid output reagents.