A-iPSC-AG4-CL82
Genomic DNA from patient-derived cell lines (hiPSC) was used for DNA extraction using DNeasy Blood and Tissue kit (Qiagen) and whole exome sequencing (WES) was performed at the Mayo Clinic Advanced Genomics Technology Center. Paired-end libraries using 1.0 μg of gDNA were prepared according to the standard manufacture’s with the Agilent Bravo liquid handler. The concentration and size distribution of the completed libraries was determined using an Agilent Bioanalyzer DNA 1000 chip and Qubit fluorometry (Invitrogen). Whole exome capture was carried out using 750 ng of the prepped library following the protocol for the Agilent’s SureSelect Human All Exon v5 + UTRs 75 MB kit (Agilent). The purified capture products are then amplified using the SureSelect Post-Capture Indexing forward and Index PCR reverse primers (Agilent) for 12 cycles.