Analysis of expressed sequence tags from a significant livestock pest, the stable fly (Stomoxys calcitrans), identifies transcripts with a putative role in chemosensation and sex-determination
Antennae and maxillary palps (a/mp), thoraces, and proboscides (prob) of adult females and males (0 – 4 d) were dissected separately from specimens frozen on dry ice, and were stored in RNAlater*-ICE (Ambion) prior to further manipulation.  Heads with antennae (ant), maxillary palps (mp), and prob removed, referred to as ‘clean’ heads (ch), were also obtained from these specimens and were stored at -80*C until further processed.   A double-stranded cDNA library was constructed using the SMART cDNA synthesis system (BD Clontech, Mountain View, CA) and was subsequently normalized using the Trimmer-Direct cDNA normalization kit (Evrogen, Moscow) under contract with Evrogen.