Targeted RNA-seq of 0.001% ME-1 cells
Targeted RNA-seq libraries were generated using a pool of target specific primers containing 12 nt unique molecular indices (UMIs) to individually tag RNA molecules of interest during reverse transcription, follwed by targeted PCR of the barcoded cDNA, and then library construction.  The primer pool used in this library targeted ABL1, WT1, PRAME, CBFB-MYH11, PML-RARA bcr1/2, PML-RARA bcr3, BCR-ABL1 p190, BCR-ABL1 p210, and RUNX1-RUNX1T1.