m6a-seq of mus muscles: homogenized brain
Total RNA was extracted from adult mouse forebrain whole tissue (HOM) and synaptosomes (SYN) with Sepasol (Nacalai Tesque) and depleted rRNA (RiboMinus Eukaryote System v2). 500 ng fragmented RNA was used as input RNA for m6A immunoprecipitation and 4 ng eluted RNA was used for RNA-seq library preparation (IP libraries) using SMARTer Universal Low Input RNA sequencing Kit (Takara) and Ion Total RNA-seq Kit v2 (Life Technologies). Single-end sequencing was performed on Ion Proton System. 4ng Input RNA was used to construct cDNA libraries (INPUT libraries) and sequenced in parallel.