Whole Exome Sequencing of Esophageal dysplasia
Fragmentation was carried out by hydrodynamic shearing system (Covaris, Massachusetts, USA) to generate 180-280bp fragments. DNA fragments with ligated adapter molecules on both ends were selectively enriched in a PCR reaction that hybridized with liquid phase with biotin labeled probe. Total 50Mb of DNA sequences of 334,378 exons from 20,965 genes were captured.