Sequencing of highly salt tolerant wild species of rice
Genomic DNA (1.6 Âľg) was end-repaired (NEBnext ultra II end repair kit, New England Biolabs, MA, USA), cleaned up with 1x AmPure beads (Beckmann Coulter,USA). Adapter ligations (AMX) were performed for 30 minutes using NEB blunt/ TA ligase (New England Biolabs, MA, USA). Library mix was cleaned up using 0.4X AmPure beads (Beckmann Coulter,USA) and finally eluted in 15 Âľl of elution buffer. A total of 742.5 ng of sequencing library was obtained and used for sequencing.