16S rRNA gene amplicon data of a filtered soil sample
100 g soil sample of the Experimental Botanical Garden Goettingen (Germany) was solved in 500 ml ddH2O and subsequently filtered with a 2.7 micrometer filter. DNA was extracted with the PowerMax Soil DNA isolation kit (MO BIO Laboratories). The 16S rRNA gene V3-V4 region was amplified using the primers S-D-Bacter-0341-b-S17 and S-D-Bact-0785-a-A-21 (Klindworth et al., 2013). The sequencing library was generated with the Nextera XT DNA library prep kit (Illumina). For sequencing, the MiSeq reagent kit version 3 (Illumina) was used.