16S rRNA gene amplicon data of an enrichment culture (after 68 h of incubation)
100 g soil sample of the Experimental Botanical Garden Goettingen (Germany) was solved in 500 ml ddH2O and subsequently filtered with a 2.7 micrometer filter. 5 ml were added to 45 ml M9-HEPES. After a total of 68 h of incubation, DNA was isolated using the MasterPure complete DNA and RNA purification kit (Epicentre). The 16S rRNA gene V3-V4 region was amplified using the primers S-D-Bacter-0341-b-S17 and S-D-Bact-0785-a-A-21 (Klindworth et al., 2013). The sequencing library was generated with the Nextera XT DNA library prep kit (Illumina). For sequencing, the MiSeq reagent kit version 3 (Illumina) was used.