RNASeq of Secale cereale
10 g of total RNA of each bulk were used for cDNA synthesis. Full-length-enriched cDNA was normalized using duplex-specific nuclease (DSN) from Evrogen (Moscow 117997, Russia) Trimmer-2 cDNA normalization kit based on nucleic acid hybridization kinetics. After optimization, the cDNA libraries were prepared for Illumina 100bp paired end sequencing on an Illumina HiSeq2000 following standard procedures (Illumina, Inc., San Diego, CA, USA).