SynHiC RNAseq replicate 3
Single-end non-strand-specific RNA-seq of the Syn-HiC and BY4742 were performed using Illumina Nextseq and standard TruSeq preparations kits, after depletion of ribosomal RNA. Reads were mapped using Bowtie2 (quality >30) to the reference S. cerevisiae BY4742 and Syn-HiC genome. Gene differential expression was measured using DESeq2, with standard parameters.