Long-read (MinION) genome sequencing of Perkinsus olseni 00987-12
Genomic DNA was purified using the Genomic-Tip 100/G extraction kit (QIAGEN). Large genomic DNA fragments (>1.5 kbp) were enriched using a 1X volume of AMPure XP beads (Beckman-Coulter) in 11% PEG-8000 (Sigma), 1 mM EDTA, 10 mM Tris-HCl and 1.6 M NaCl and further purification using 70% ethanol. Enriched large fragment genomic DNA was then end-prepped for ONT sequencing using the Sequencing Ligation kit (LSK-108) and sequenced for 24 h on a MinION 9.4.1 Rev. C.