16S rRNA Metagenome of oil-based-drilling-mud polluted site bioremediated by a consortium of oil-degrading bacteria supplemented by mineral nutrients
Soil DNA was extracted using Nycodenz solution. Then fragments of 507 nucleotides of the 16S ribosomal gene from the V3 to V4 region was amplified using universal primers. The amplicons were sequenced by Illumina MiSeq.