Uromyces transversalis transcriptiome of germinating urediniospores
RNA was extracted from urediniospores isolated from infected leaves of Gladiolus communis. PolyA-enriched total RNA was used to prepare a library optimized for Roche/454 Titanium sequencing that included partial normalization with double-stranded nuclease and PCR suppression of misligated products. Reads were cleaned of adaptor sequences using http://sourceforge.net/projects/estclean. See doi: 10.1111/pbi.12068