RNA sequencing of UT-SCC-1A
RNA was extracted from cell lines in logarithmic growth under ambient oxygen and TruSeq Stranded mRNA libraries were then prepared from 500 ng input RNA using v4 chemistry. PCR enrichment was limited to 10 cycles. Final libraries were quantified using a Qubit HS DNA Assay Kit (Thermo Fisher Scientific) and quality was assessed with a 2100 Bioanalyzer (Agilent Technologies; Santa Clara, CA). Libraries were normalized, pooled equimolarly and sequenced on 3 lanes of a HiSeq 2500 flow cell with 125 bp paired-end sequencing (Illumina; San Diego, California).