RNA-Seq of Homo sapiens: 70 years old Female, salivary duct carcinoma
RNA-Seq libraries were prepared using the KAPA Stranded RNA-Seq with RiboErase sample preparation kit (KAPA Biosystems). Total RNA (100 ng) was ribosomal RNA-depleted and fragmented, followed by first and second strand synthesis, A-tailing, adapter ligation, and PCR amplification (using 11 cycles). Final libraries were quantified using the KAPA Library Quantification Kit (KAPA Biosystems), Qubit Fluorometer (Life Technologies), and 2100 BioAnalyzer (Agilent) and were sequenced on a HiSeq2500 v4 chemistry sequencer (Illumina) using 2 × 125 bp cycles.