ATAC-seq of Homo sapiens:human iPSCs
HFF (50 000), reprogrammed HFF (50 000) at day 7 and 14, and TRA-1-60+ cells (50 000) purified from reprogrammed HFF at day 21were lysed in a lysis buffer (10 mM Tris HCL pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL-CA630). TransNGSTn5 DNA library pre-Kit for Illumina was used to prepare DNA library for sequencing.