Complete genome of two Rhizobium and two Mesorhizobium and comparative analysis for correct classification
The DNA Genogenical Wizard extraction kit (Promega) was used to extract genomic DNA according to the manufacturer's instructions. As a set of DNA were measured fluorometrically (Qubit 3.0, Qubit dsDNA Broad Range Assay Kit, Life Technologies, Carlsbad, CA, USA). DNA from the DNA was determined by agarose gel electrophoresis with a DNA content of 0.8% (w / v) and subsequent with SYBR DNA Secure DNA Stins (Invitrogen, Carlsbad, CA, USA). Sequencing was performed using the Miseq Reagent v2 kit 2 x 150 bp