Nextera MP Gel Plus
High molecular weight (HMW) DNA was extracted from the nuclei using a modified CTAB extraction method (Doyle and Doyle, 1990) at the University of Saskatchewan. A sample of the HMW DNA was sent to the University of Illinois Roy J. Carver Biotechnology Center for sequencing to a depth of 333x from pair-end and mate-pair libraries following the same protocol as Chen et al (2019)