RNA-seq of ovary: self-pollination
The mRNA was enriched and purified by magnetic beads with oligo T (dT), cleaved and synthesized first-strand cDNA using random hexamers. Then the double-strand cDNA libraries were purified by AMPure XP system (Beckman Coulter, Beverly, USA). The transcriptome sequencing was performed by Illumina HiSeqTM 2000 sequencing platform in Novogene (Tianjin, China). The adaptor and low-quality sequences (Q < 20 or less than 35 bp) were removed in raw reads. Then the clean reads were assembled into Trinity assembler (Grabherr et al, 2011).