RT112 CRISPRi Screen
Genomic DNA was used to amplify the lentiviral integrations of the screen library. Barcoding in the FW primer was used to distinguish samples within the experiment:  CGTGAT: t=0, replicate 1  ACATCG: t=0, replicate 2  GCCTAA: t=0, replicate 3  TGGTCA: t=1 -Dox, replicate 1  CACTGT: t=1 -Dox, replicate 2  ATTGGC: t=1 -Dox, replicate 3 GATCTG: t=1 +Dox, replicate 1 TCAAGT: t=1 +Dox, replicate 2 CTGATC: t=1 +Dox, replicate 3