Trigona ventralis hoozana transcriptome sequencing
RNA was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA), according to the manufacture’s specifications. Finally, the RNA pellet was suspended in 50 μl RNase-free water. The whole bee was crashed in liquid nitrogen by mortar/pestle and was suspended in 10 mM Tris/EDTA buffer containing 100 μg proteinase K (Qiagen) for two hours at 56°C with shaking. After, The genomic DNA was extracted using a DNeasy Blood and Tissue Kit (Qiagen).  RNA and DNA sequencing libraries were constructed at Genomics biosci & biotech (Taiwan), using RNA TruSeq library preparation kits and TruSeq DNA PCR-Free Library Preparation Kit. RNA and DNA library quality and quantitative was checked with Bioanalyzer High sensitivity DNA chips and QIAseq library quant assay kit.