Illumina of sludge samples: Anaerobic phenol biodegradation
16S NGS analysis was performed at Stab Vida Lda (Lisbon, Portugal). Sequencing targeted the V5 and V6 regions (250bp) of the 16S rRNA gene using the primers 799F (5-AACMGGATTAGATACCCKG-3) and 1175R1 (5-ACGTCRTCCCCDCCTTCCT-3) (Bonder et al. 2012). Samples were then sequenced with MiSeq Reagent Kit Nano though the Illumina MiSeq platform.