DNase-seq of W7 gDNA from developing secondary xylem
Genomic DNA was isolated and digested with 7.5 mU, 10 mU and 13 mU DNase I for 10 min at 37C. The reaction was quenched with EDTA, DNA was extracted and run on an agarose gel. Fragment sizes of 50 - 300 bp across all three digestions were gel-purified