Illumina HiSeq 2000 sequencing; Whole transcriptome analysis in nicotiana benthamiana WT and transgenic lines presenting spontaneous systemic posttranscriptional silencing (PTGS) of a GFP transgene (line GFP6.4 described in Kalantidis Plant J 2006). Change in gene expression was analysed in WT versus GFP6.4 before initiation, during spreading and maintenance of GFP-PTGS.
Whole transcriptome analysis in nicotiana benthamiana WT and transgenic lines presenting spontaneous systemic posttranscriptional silencing (PTGS) of a GFP transgene (line GFP6.4 described in Kalantidis Plant J 2006). Change in gene expression was analysed in WT versus GFP6.4 before initiation, during spreading and maintenance of GFP-PTGS.