Pacbio-Seq of Eucomia ulmoides
The DNeasy Plant Mini Kit kit (TIANGEN, Beijing, China) was used to extract high-quality genomic DNA from young leaves of haploid Eucommia ulmoides. For PacBio sequencing, a Single-Molecule Real Time (SMRT) bell library with a length of 40 kb insert fragment was constructed according to the manufacturers protocol (PacBio, CA).