WGS of Verticillium dahliae: isolate 150
Verticillium dahliae isolates were grown in potato-dextrose-broth (PDB, Difco, Detroit, USA) at 25C for 7days. Then, mycelia were filtered, lyophilized and ground to a fine powder. DNA was extracted from 50 mg ground mycelium and purified. Purity and quality of DNA samples were checked both by electrophoresis and by using a Qubit 2.0 fluorometer (Invitrogen, UK). Genomic DNA from the five V. dahliae isolates were PE sequenced (2 x 150 bp) achieving a depth of coverage in the genomic sequencing  greater than 100X.