16S rRNA bacterial composition,  Control diet Phase III, sample-25
Fecal sample collected from nursery piglet fed a standard Phase III diet (sample #25). Sample kept at -20C until microbial genomic DNA extractions. Microbial genomic DNA was extracted using repeated beating followed by Qiagen stool kit. PCR amplification of bacterial V1-V3 regions of 16S rRNA genes using 27F-519R primers. Sequencing using Illumina MiSeq2X300.