DNA-Seq of negative control: filter sterilized aqueous ammonium chloride March 6, 2013
DNA was extracted with the Qiagen Blood and Tissue kit. The V4 hypervariable region of the bacterial 16s rRNA was amplified with primer set 515f/806r. For some samples, part of the fungal ITS gene was also amplified with primer set ITS1f/ITS2. Amplicons were sequenced on an Illumina MiSeq platform.