RNA-seq of 5-dpf perithecia of Neurospora crassa stc1 mutant (FGSC17465 x FGSC17464)
The total RNA  was extracted and purified with the RNAprep Pure Plant Kit (Tiangen Biotech, Beijing, China). Poly(A)+ mRNA was enriched from total RNA using immobilized oligo (dT). RNA-seq libraries were prepared using the NEBNext? Ultra? Directional RNA Library Prep Kit (NEB, USA) as described by the manufacturer, and sequenced by Illumina HiSeq? 2500 with a 2¡Á150 bp paired-end read mode.