Spiroplasma poulsonii MSRO genome
DNA was extracted by phenol chloroform extraction. Le library was prepared with the PacBio DNA Template Prep Kit 2.0 (3 to 10 kb) with 3 ug of genomic DNA with an expected insert length of 25 kb. Sequencing was performed with a movie of 180 min with the P4 C2 chemistry kit with size selection with the SMT MagBead kit. The loading concentration was 0.9 nM in a SMRT cell v3.