Ogataea parapolymorpha CBS 11895
Genomic DNA of CBS 11895 was isolated from a stationary overnight culture grown in YPD medium using the QIAGEN Genomic-tip 100/G kit (Qiagen, Hilden, Germany) according to manufacturer’s instructions, and quantified using a Qubit® 2.0 Fluorometer (Thermo Fisher Scientific). 10 µg of genomic DNA was sequenced by Novogene Bioinformatics Technology (Yuen Long, Hong Kong) on a HiSeq 2500 (Illumina, San Diego, CA) with 150 bp paired-end reads using TruSeq PCR-free library preparation (Illumina). In order to verify absence of plasmid pUDP046, the sequencing reads were mapped to reference genome of O. parapolymorpha CBS 11895 (accession GCF_000187245.1) ) using the Burrows–Wheeler Alignment tool (BWA) and further processed using SAMtools