WGS of homo sapiens: adult male Whole Blood
Genomic DNA input into shearing was reduced from 3 ug to 100 ng in 50 uL of solution. Illumina paired-end adapters were replaced with palindromic forked adapters with unique 8-base index sequences embedded within the adapter. Size selection was performed using Sage's Pippin Prep, with a target insert size of 370 bp +/- 10%. Libraries were quantified with qPCR using the KAPA Library Quantification Kit, and normalized to 1 nM. Cluster amplification of the templates was performed according to the manufacturer's protocol, using the Illumina cBot. Flowcells were sequenced on HiSeq X following the manufacturer's protocol.