RNA-seq of D. ferrophilus IS5: lactate-starved and -sufficient conditions
Total RNA was prepared using NucleoSpin RNA Kit (TakarBio). rRNA was removed with a Ribo-Zero Magnetic Kit (Gram-Negative Bacteria) and a cDNA library was prepared for sequencing using the TruSeq Stranded mRNA Library Prep Kit (Illumina) following the manufacturer's guidelines. RNA quality was checked with an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The cDNA library was sequenced on a HiSeq 2500 instrument (Illumina) generating between 51 and 54 million 100-bp pair-end reads per library.