WGS of Acinetobacter baumannii: carbapenems resistant.
Total DNA was extracted using PureLink® Genomic DNA mini kit from ThermoFisher and used to prepare multiplexed DNA libraries with the Nextera XT Dual Index Primer kit (Illumina, Inc.) and the Kapa Library Amplification Kit. Multiplexed libraries were pooled into a single sample and then purified and size selected to improve the fragment size distribution using the SPRIselect Reagent kit. Paired-end 300-bp sequencing of the pool was performed on the Illumina MiSeq platform using the MiSeq V3 600 cycles chemistry.