SP6C4 genome seuqencing
Bacteria was grown in TSB amended with 20% sucrose, transferred to MS medium (soya flour, 20 g; mannitol, 20 g; agar, 20 g per 1L water)19 and cultured at 30C for 7 days. Spores were collected into 1.5-mL Eppendorf tubes and genomic DNA was extracted by using a CTAB lysozyme procedure20. Genomes were sequenced with the Pacific Biosciences RSII (PacBio, USA) single molecule real time (SMRT) system and non-hybrid hierarchical genome (HGAP version 2.3) pre-assembly Quiver (http://www.pacificbiosciences.com/devnet/). Whole genome alignment used the Web ACT program (Artemis Comparison Tool, Center for Bioinformatics). Circular genome mapping and analysis were conducted with BLAST Ring Image Generator (BRIG) v 0.95 (http://sourceforge.net/projects/brig/)