Amplicons of aclB gene from the triplicate samples
With the paired primers sets as the format "Adaptor-key for pyrosequencing recognition-barcodes-primer sequences":  aclB forward primer: CCATCTCATCCCTGCGTGTCTCCGAC-TCAG-xxxxxxxxxx-TGGACMATGGTDGCY aclB reverse primer: CCTATCCCCTGTGTGCCTTGGCAGTC-TCAG-ATAGTTKGGSCCACCTCTTC