WXS of Rattus norvegicus:adult male liver
To profile the somatic mutation spectrum in rat HCC model, we performed whole-exome sequencing on 9 liver tissues and 3 HCC tissue, including 3 from normal mock group, 3 from liver hepatitis group (LH), 3 from liver cirrhosis group (LC), and 3 from HCC group. All methods for library construction were performed as described in the SeqCap EZ Library protocols (Roche NimbleGen, Madison, WI). Genomic libraries were prepared using the SepCap EZ Developer Library for rats, per the manufacturers instructions (Roche NimbleGen). Each exome was sequenced using the 100 bp paired-end protocol on an Illumina HiSeq 2000 DNA Analyzer to produce approximately 12 to 20 Gb of sequence per exome.