Genome sequencing of Phellinus noxius isolate Pn103
Phellinus genomic DNAs were sheared to major at ~300 bps (spanning 200-700bps) using Covaris microTUBE AFA. Illumina PE DNAlibraries were constructed using the single index kit. After AmPure size selection, the final libraries showed smear at 250-800bps with major distribution at ~450 bps. Illumina sequencing was carried out on HiSeq 2500 at PE2*251nt.  All NGS preparation and sequencing were conducted at the High Throughput Genomics Core at BRCAS, Academia Sinica, Taiwan.