Illummina sequencing of Mytilus galloprovincialis hemolymph
eukaryotic mRNA was extracted from total RNA using oligo (dT) magnetic beads and cleaved into short fragments using fragmentation buffer. A cDNA library compatible with the Illumina NGS technology was then prepared from the fragmented mRNA via reverse transcription, second-strand synthesis and ligation of specific adapters (paired-ends) after cDNA purification using the QIAquick PCR Purification Kit (Qiagen). The amount of cDNA in each library was quantified through spectrofluorometric analysis using the Qbit system. Next-generation sequencing was performed using Illumina HiSeq 4000 technology