Transcription factors in light and circadian clock signaling networks revealed by genome-wide mapping of direct targets for Neurospora WHITE COLLAR COMPLEX
DNA was end repaired and ligated to adapters (Pomraning, K. R., K. M. Smith, and M. Freitag. 2009. Genome-wide high throughput analysis of DNA methylation in eukaryotes. Methods 47:142–150.). The 200- to 500-bp fragments were gel purified and amplified with 20 cycles of PCR using Phusion polymerase (Finnzymes Oy). The PCR products were gel purified before sequencing.