WGS of Ralstonia solanacearum P673, a strain capable of infecting tomato plants at low temperature
Ends of fragmented DNA are made blunt. Adaptors are added and tagged fragments enriched with PCR. A 5biotin tag is added to immobilize the library to streptavidin-coated beads. After nick repair, the non-biotinylated strand is released and used as a single-stranded template DNA library