V4 hypervariable region of the 16S rRNA gene of Mus musculus: adult male gut microbiota, vehicle treatment
Total microbial DNA extracted from fecal pellets (MoBio PowerSoil DNA Isolation Kit). The V4 hypervariable region of the 16S rRNA gene was amplified using primers F515 and R806. Multiplex PCR was performed with dual indices (Kozich et al. 2013, Appl. Environ. Microbiol. 79(17):5112) and the pooled normalized library was sequenced using the Illumina MiSeq sequencing platform with the reagent kit V2.