Strombus gigas cDNA library 454 sequencing
Total RNA was isolated from 12 individual queen conch (Strombus gigas) tissue samples (neural ganglia, gonad, and digestive gland from each of four conchs) using TRIzol (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s protocol; 200 ng each RNA sample were pooled and used as the template for the cDNA library construction and normalization using the SMART (Clontech/Takara Bio USA, Madison, WI, USA) and Trimmer (Evrogen Joint Stock Company, Moscow, Russia) kits, respectively.