Metagenomic sequencing of total RNA from posterior lobe of liver tissues of 309 individual Lymnaea stagnalis, consisting of pooled wild-caught and laboratory strain "UoN_dex" snails.
Total RNA was provided to Edinburgh Genomics (Edinburgh, UK) for paired-end sequencing using the Illumina platform. Following ribosomal RNA depletion using RiboZero Gold (Illumina), TruSeq stranded total RNAseq libraries (Illumina) were prepared using standard barcodes. HiSeq 4000 150PE to yield at least 290M + 290M reads (1 lane)