Cell free DNA seqeuencinf of kidney transplant recipient urine: Sample JL.URINE.36B
Cell free DNA was extracted from 1 mL of urine supernatant using the Qiagen Nucleic Acid Kit (Qiagen, Valencia, CA).  A single stranded library preparation was performed using biotinylated adapters (Burnham et al., Sci Rep  6:27859, 2016, PMID: 27297799). Samples were sequenced on the lllumina NextSeq platform (75 base pair by 75 base pair). Further details are found in Burnham et al., Nat Comm 9(1):2412, 2018, PMID: 29925834. 