sRNAseq Arabidopsis + IR-CFA6/HRPL
Stable transgenic lines expressing IR-CFA6/HRPL constructs were generated by transforming Arabidopsis (accession Col-0) plants using Agrobacterium mediated-floral dip method. The IR-CFA6/HRPL construct is composed of 250 bp regions of Pto DC3000 genes, cfa6 (1-250 nt) and hrpl (99-348 nt), aligned in sense and antisense directions with the intron of the petunia chalcone synthase gene (CHSA) in between. Small RNA libraries were constructed and sequenced from four- to five-week old leaves of IRCFA6/HRPL#4.