Hindgut microbiome of Triatoma infestans by 16S deep sequencing
The V3-V4 region of the 16S rRNA gene was amplified using the published Illumina primers and protocol (see ref 35) producing amplicons of approximately 460 base pairs. Libraries were quantified using the Agilent 4200 TapeStation (Agilent Technologies), pooled in equimolar amounts, then sequenced on the Illumina MiSeq platform by a paired-end 2x250 sequencing run at the High Throughput Sequencing Facility at the University of North Carolina at Chapel Hill.