JDP2 ChIP-seq 30 min TNFa
Typically, 15-30 million HUVECs were cross-linked (10 min, 20°C) in 1% paraformaldehyde (Electron Microscopy Science) at the appropriate times after TNFα induction, nuclei were isolated and chromatin prepared, fragmented, and collected using the ChIP-It High Sensitivity kit (Active motif); immuno-precipitations were performed on aliquots of ~25 μg chromatin using antisera targeting acetylated lysine 27 of histone H3 (Abcam; ab4729.