sgRNA sequencing library of HeLa HMGCR-Clover RNF145 KO, sorted
DNA was extracted using the Quick-gDNA MicroPrep kit (Zymo Research). Individual integrated gRNA sequences were amplified by PCR (primers: sgRNA_outer_F: GCTTACCGTAACTTGAAAGTATTTCG; sgRNA_outer_R: CGAGACTAGTGAGACGTGCTAC). Illumina adaptors  were added in an additional round of PCR (P5-sgRNA_inner_F: AATGATACGGCGACCACCGAGATCTACACTCTCTTGTGGAAAGGACGAAACA CCG; P7-index-sgRNA_inner_R: CAAGCAGAAGACGGCATACGAGATnnnnnnnGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTCCATTTGTCACGTCCTGCACG). Sequencing was carried out using the Illumina MiniSeq platform (Illumina sequencing primer (5'-3'): CTTGTGGAAAGGACGAAACACCG..