UMUC3 shRNA and CRISPR Screens
Genomic DNA was used to amplify the lentiviral integrations of the screen library. Barcoding in the FW primer was used to distinguish samples within the experiment:  CGTGAT: t=0, replicate 1 shRNA screen ACATCG: t=0, replicate 2 shRNA screen  GCCTAA: t=0, replicate 3 shRNA screen TGGTCA: t=1, replicate 1 shRNA screen CACTGT: t=1, replicate 2 shRNA screen ATTGGC: t=1, replicate 3 shRNA screen GATCTG: t=0, replicate 1 CRISPR screen TCAAGT: t=0, replicate 2 CRISPR screen CTGATC: t=0, replicate 3 CRISPR screen AAGCTA: t=1, replicate 1 CRISPR screen GTAGCC: t=1, replicate 2 CRISPR screen TACAAG: t=1, replicate 3 CRISPR screen