WGS of Cucumis sativus: infected plants with geminivirus
Leaves with typical geminivirus disease symptoms from cucumber were collected from Colima state, Mexico. The DNA extraction of collected samples was carried out with the Dellaporta DNA extraction method. Viral genomes were enriched by rolling circle amplification (RCA) using phi29 (Thermo Fisher Scientific, Waltham, MA USA). 3 g of RCA products from each plant were pooled. 1 g of pooled RCA was sequenced by Illumina NextSeq 500 platform in 2x150 configuration.