18S  amplicon sequencing of marine metagenome: Skidaway River, GA (USA)
Libraries were prepared and sequenced by the Georgia Genomics and Bioinformatics Core at the University of Georgia. Libraries were constructed to capture the V4 region of the 18S rRNA using primers from Stoeck et al. 2010: forward (5-CCAGCASCYGCGGTAATTCC-3) and reverse (5-ACTTTCGTTCTTGATYRA-3). Amplicon libraries were prepared using an input of 2-5 ng/ul of DNA and sequenced using the Illumina MiSeq platform to produce 250+250 nt paired reads.