RNAseq of SW629 cells transduced with pLVX empty vector
Total RNA was extracted using RNeasy Plus Mini Kit from Qiagen. Ribosomal RNA was removed using the RiboGone Kit, followed by cDNA synthesis using the SMARTer Universal Low Input RNA Kit for Sequencing (Takara Bio). Libraries were prepared using the Ion Plus Fragment Library Kit and sequenced using the Ion S5 Systems (ThermoFisher) at he Analytical and Translational Genomics Shared Resource at the University of New Mexico Comprehensive Cancer Center.