Metagenomicf mouse smalll intesinal micriobiota
Total genomic DNA of ileal digesta was extracted using an E.Z.N.A.R Stool DNA Kit (Omega Bio-tek Inc., United States) according to the manufacturers introduction. DNA quantity and quality were monitored with NanoDrop 2000 (Thermo Scientific, United States) and 1% agarose gel. Totally 24 ileal digesta DNA samples were extracted and the two ileal digesta DNA from the same replicate were equally mixed and 12 samples were applied for sequencing