RNA-Seq of homo sapiens: Scoliosis Patient Muscle rep 2
Total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). RNA fragments were reverse-transcribed to create the final cDNA libraries following the NEBNext Ultra RNA Library Prep Kit (Illumina) and paired-end sequencing 150bp was performed on a Novaseq 6000.