RNA-seq of rose petal
Total RNA of rose petal in blooming stage was extracted using the mirVana miRNA Isolation Kit (Ambion) following the manufacturer  s protocol. The libraries were constructed using TruSeq Stranded mRNA LTSample Prep Kit according to the manufacturer  s instructions. Then these libraries were sequenced on the Illumina sequencing platform (HiSeqTM 2500) and 125bp paired-end reads were generated. And these samples included three biological repetitions, which constructed independent library to sequencing.