Sequencing of Hela S3 phase replicate 1
The libraries were prepared following the Illumina’s protocol for ChIPSeq library construction. The DNA sample overhangs were first converted into phosphorylated blunt-ends using T4 DNA polymerase, Klenow DNA polymerase and T4 polynucleotide kinase. An adenine residue was then added in 3’ position by the Klenow (exo-) polymerase. After ligation of the Illumina adapter (diluted 1/10), the mix was gel-purified to select 200-400 bp fragments; a final PCR amplification step (14-18 cycles) was performed using specific primers to complete the flanking sequences.