16S amplicon sequencing of Peltigera frigida lichen
PCR of V4-V5 region of bacterial 16S, with primers that exclude cyanobacteria (Unibac-II-515F and 902R). Band of interest was purified from agarose gels and sended to Macrogen Inc. (Korea) for Illumina MiSeq 300bp x 2 amplicon sequencing.