ChIP-seq of Eucalyptus grandis: H3K4me3 of sample W2
Developing secondary xylem tissue was finely ground in liquid nitrogen and chromatin was extracted as described by Li et al. (2014). ChIP was performed with anti-H3K4me3 using the protocol of Adli & Bernstein (2011), without ChIP DNA amplification. Illumina ChIP-seq libraries of immuno-enriched and control (input) samples were prepared and sequenced (SE50 reads; 200 bp library insert) by the Vincent L. Coates Genomics Sequencing Laboratory, University of Berkeley, CA.