Bacterial diversity analysis: soil sample
Gemonic DNAs were extracted from the soil samples. Primers 515F and 806R were used to amplify the V4 region of the 16S rDNA. A bar code sequence was added in 806R. PCR was conducted in triplicate, and the products were pooled for each subsample. A composite DNA sample for sequencing was created by combining equimolar ratios of amplification products from each sample.