Strain-Level Genetic Diversity of a Cyanobacterial Population
Oligonucleotides andrecommended adaptors for 454 library construction were ordered from Integrated DNATechnologies (IDT, http://www.idtdna.com). 454 libraries were created for both HMW and LMWsamples and were tagged with MIDs. Libraries were constructed based on the 454 manual.Sequencing was performed at the Stanford Genome Center. HMW and LMW libraries werepooled and sequenced on a full ungasketed plate with 454 Titanium technology. 441.625Mb ofdata was generated, consisting of 1,242,000 reads. Run statistics met or exceeded all qualitychecks.