Euschistus heros while transcriptome sequencing
Eggs, pool of ninph and adults of E. heros were used for total RNA extraction using the Trizol reagent (Invitrogen Life Technologies) according to the manufacturer instructions. The cDNA library preparation and Illumina sequencing were conducted at the Laboratory of Functional Genomics Applied to Agriculture and Agri-Energy, at the University of So, Brazil. The TruSeq RNA Sample Prep kit (Illumina) protocol was used to construct the cDNA library, following manufacturer instructions. dNTP mix containing dUTP was used for second strand cDNA synthesis, and once adapter ligation was done, second strand cDNA was digested, allowing strand-specific sequencing. Ampure XT beads were used to select the library size, and Agilents Bioanalyzer High Sensitivity DNA Chip was used for library quantifications.