RNA-Seq under sulfate limitation replicate 1
The RNA concentration of all samples were measured in duplicate using the Qubit BR RNA assay. The RNA quality and integrity was conrmed for selected samples using TapeStation with RNA ScreenTape (Agilent Technologies). Ribosomal rRNA using was removed using the Ribo-zero Magnetic kit (Illumina Inc.) according to the manufactures instructions. Any potential residual DNA was removed using the DNase MAX kit (MoBio Laboratories Inc.) according to the manufactures instructions. After rRNA depletion and DNase treatment the samples were cleaned and concentrated using the RNeasy MinElute Cleanup kit (QIAGEN) and sucessfull rRNA removal conrmed using TapeStation HS RNA Screentapes (Aligent Technologies). The samples were prepared for sequencing using the TrueSeq Stranded Total RNA kit (Illimina Inc.) according to the manufactures instructions. Library concentrations were measured using Qubit HS DNA assay and library size estimated using TapeStation D1000 ScreenTapes (Aligent Technologies). The two samples were pooled in equimolar concentrations and sequencing on an Illumina HiSeq2500 using a 1x50 bp Reagent Kit and single read (SR) cluster kit (Illumina Inc).