whole genome sequencing (DNA WGS) of human: PANC-1 pancreas duct carcinoma cells
1ug DNA was collected and purified from samples using DNeasy Blood & Tissue kit (Qiagen). The DNA library was further prepared according to the Illumina TruSeq DNA PCR-free library preparation guide. DNA was fragmented by covaris system into 300-400bp dsDNA with 3ĄŻ or 5ĄŻ overhangs, repaired to blunt end and selected by size. DNA was then adenylated at the 3ĄŻend, indexed by adapters ligation, and validated by quality control. Paired-end sequencing was performed to reach in average 30X of genome coverage.