RNA-Seq of Solanum lycopersicum, Solanum pennellii IL4-3 P4 leaf
cDNA was amplified from total RNA using Clontech SMARTer kit (634926, TaKaRa Bio USA); cDNA was fragmented using Fragmentase (M0348, NEB) and porcessed for the addition of Illumina TruSeq universal adapters; Libraries were amplified using Phusion HiFi Polymerase mix (M0531, NEB) and pooled to a final library concetration of 20 nM (pools of 8 indexed samples/library).