16S rRNA sequencing of gut microbiota:Monopterus albus
The barcoded fusion forward primer 338F (5-ACTCCTACGGGAGGCAGCA-3) and the reverse primer 806R (5-GGACTACHVGGGTWTCTAAT-3) were used to amplify the V3-V4 hyper variable region of the 16S rRNA gene. The Barcoded V3-V4 amplicons were sequenced using the paired-end method on Illumina MiSeq platform (Illumina, USA) following the standard protocols.