DBY10147 whole genome sequencing CA0001.SAM1.EXP1
Briefly, 10 ug of yeast genomic DNA were sonicated to fragment sizes below 2000 bp, concentrated and end-repaired using the End-It DNA repair kit (EPICENTRE Biotechnologies).  End-repaired DNA was A-tailed with GoTaq DNA polymerase (Promega) and ligated to Illumina adapters (QuickLigase, NEB).  Ligation products between 300-400bp were excised from a 6% polyacrylamide gel, eluted and ethanol precipitated.  Fragment libraries were PCR amplified, cleaned following AMPure (Agencourt) and Qiaquick PCR clean-up procedures, and submitted for sequencing. We prepared two such libraries for each strain.    We collected 13,555,852 and 13,901,121 single-end, 36 bp, quality-filtered reads from DBY11331 and DBY10147, respectively, using the Illumina Genome Analyzer II platform.