RNA-seq of Mylochromis mola skin
RNA-seq libraries have been prepared using the TruSeq Stranded mRNA Library Prep Kit (Illumina) according to protocol. The final libraries were amplified using 15 PCR cycles. Quantification and quality-assessment was performed using a Bioanalyzer 2100 (Agilent Technologies, Palo Alto, CA). Indexed DNA libraries were normalized and pooled in equal volumes. Paired-end 50bp (Pundamilia nyererei and Haplochromis sauvagei) and 150bp (Pseudotropheus demasoni, Pseudotropheus. cyaneorhabdos, Me. auratus, Copadichromis boreleyi and Mylochromis mola) libraries were sequenced on an Illumina HiSeq 2500 (Pundamilia nyererei and Haplochromis sauvagei) or HiSeq X Ten platform (Pseudotropheus demasoni, Pseudotropheus cyaneorhabdos, Melanochromis auratus, Copadichromis boreleyi and Mylochromis mola).