RNA-seq of A2780 cells: 15nm drug
A2780 cells, already resistant to 10nM D4-9-31, were conditioned to continuous 105nM D4-9-31 exposure level and maintained in RPMI-1640 medium supplemented with 10% FBS and 15nM D4-9-31. Total RNA was extracted using a Zymol Research Direct-zol Miniprep kit and converted into cDNA.