Bacterial interactions in Nematostella vectensis
Genomic DNA was extracted from individual polyps with the DNeasy Blood & Tissue Kit (Qiagen) as described in the manufacturer s protocol. Eluation was performed in 50  l. Extracted DNA was stored at -20  C until sequencing. Prior sequencing the variable regions 1 and 2 (V1V2) of the bacterial 16S rRNA genes were amplified according to the previously established protocol using the primer 27F and 338R (Rausch P et al., 2016)