Schistosoma mansoni male adults genomic DNA solexa sequencing 1
Solexa sequencing was performed at the sequencing facilities of GenomiX Montpellier (France) on a Genome Analyzer II (Illumina) by single end sequencing according to the manufacturers protocol. Twenty ng of MNase fragmented DNA from each sample was repaired to generate phosphorylated blunt ends. An adenine was added to the 3' end of the blunt phosphorylated DNA fragments. Illumina's adapters were ligated to the DNA fragments. Size selection was performed using a 2% agaraose gel and a slice was excised at 200 bp corresponding to an insert size of 140 bp. The DNA extracted from the gel was then used as a matrix for 18 cycles PCR using Illumina's PCR primers. Each library was purified and quantified using a DNA1000 Chip on a 2100 BioAnalyzer (Agilent Technologies). These libraries were denaturated using NaOH and then diluted to a final concentration of 2 pM. One hundred microl of these diluted libraries were used for clustering on the Cluster Station using Clustering Kit V.2 (Illumina) and sequencing on the Genome Analyzer using a 36 Cycle SBS Kit V.3 (Illumina). A total of 8,600,198 sequence reads were produced with GAPipeline 1.3.