Amplicon sequencing of arbuscular mycorrhizal fungi from intact and restored forest in Hakalau, HI.
Dried soil was extracted using the MP Bio FastDNA spin kit. A two-step PCR reaction, using Fluidigm tag CS2 at the 3' end, was performed to amplify the small subunit(SSU) of arbuscular mycorrhizal fungi's ribosomal RNA (rRNA). PCR products were cleaned using AMPure XP beads, normalized with Charm Biotech Just-a-Plate, and paired end sequenced for 600 cycles on the Illumina Miseq platform.