Total RNA sequenced from Atlantic salmon with cardiomyopathy syndrome (CMS)
RNA was DNase treated using TURBO DNA-free (Applied Biosystems/Ambion) and reverse transcribed/amplified using the QuantiTect kit (QIAGEN) according to manufacturer’s instructions. Approximately one microgram of RNA was used as template in each reaction and the multiple displacement amplification (MDA) was allowed to proceed for two hours. Total cDNA from the three reactions was combined in equal amounts and a library with a Multiplex Identifier (MID) tag was prepared according to the GS FLX Titanium General Library Preparation Method Manual. The library was titrated and amplified using the large volume emulsion preparation and finally sequenced using a Genome Sequencer FLX instrument using GS FLX Titanium chemistry (454 Life Sciences, a Roche company, Branford, CT, USA).