Puccinia horiana transcriptome of germinating basidiospores
RNA was extracted from teliospores isolated from infected leaves of  Chrysanthemum  X morifolium. PolyA-enriched total RNA was used to prepare a library optimized for Roche/454 Titanium sequencing that included partial normalization with double-stranded nuclease and PCR suppression of misligated products. Reads were cleaned of adaptor sequences using http://sourceforge.net/projects/estclean. See doi: 10.1111/pbi.12068