RNA-Seq of mus musculus: neonatal heart
Approximately 3 ug of total RNA was subjected to poly(A) mRNA isolation with poly-T attached magnetic beads. Following purification, mRNA was fragmented into small pieces, and the average insert size for the paired-end libraries was 250 bp. Truseq library construction and sequenced on Illimuna HiSeq Xten sequencing platform with PE-150 module