RNA-seq of Lysmata vittata: euhermaphrodite phase
Total RNA from mixed tissues in carapace of  EP (mixture of 3 individuals) was extracted with Trizol Reagent (Invitrogen), followed by Illumina sequencing. Briefly, mRNA with poly (A) was isolated from total RNA using Oligo (dT) beads (Invitrogen). mRNA was broken in short fragments (about 200bp) using fragmentation buffer. These fragments were used as templates to synthesize the first-strand cDNA with random hexamers, then second-strand cDNA was synthesized. Adaptors were ligated to second-strand cDNA following by IlluminaHiseq sequencing (HiSeq 4000 SBS Kit (300 cycles), Illumina).