RNA-seq of Flax seedlings (cv. Regina) 24 h after Fusarium oxysporum treatment
14-day old flax seedlings (pathogen resistant cv. Nike and pathogen susceptible cv. Regina) were treated with a pathogenic strain of Fusarium oxysporum and samples (whole plants) were collected 24 h and 48 h, and total RNAs were isolated (for control, non treated plants were used). The mRNAs were isolated from total RNA with oligo(dT) method. Then the mRNAs were fragmented under certain conditions. Then first strand cDNA and second strand cDNA were synthesized. cDNA fragments were purified and resolved with EB buffer for end reparation and single nucleotide A (adenine) addition. After that, the cDNA fragments were connected with adapters. Those cDNA fragments with suitable size were selected for the PCR amplification. Agilent 2100 Bioanaylzer and ABI StepOnePlus Real-Time PCR System were used in quantification and qualification of those libraries.