RNA-Seq of Pseudomonas aeruginosa with pAtvR
Cells were harvest after addition of RNA protect (Qiagen), and total RNA was isolated using RNeasy minikit (Qiagen), RNA quality was assessed using Bioanalyzer. mRNA was enriched using Ribo-Zero rRNA Removal kit (Gram-Negative Bacteria) (Illumina), and rRNA depletion was confirmed by Bioanalyzer. Paired-end libraries were constructed using TruSeq RNA Library Prep Kit v2. The mean fragment size was determined by Bioanalyzer, and each library concentration was determined using KAPA Library Quantification kit (KapaBiosystems), accordingly to manufacturer’s protocol.