RNA-Seq of iPSC: human PBMC
Total RNA was extracted from iPSCs using RNA Isolation Kits (Exiqon), with DNaseI (Qiagen) treatment on column. RNA sequencing was conducted by Novogene (Tianjin, China). In brief, 1 g of total RNA from each sample was used for creating Illumina mRNA-seq libraries using a TruSeq RNA kit (version 1, set A) following selection of Poly (A) containing mRNA, fragmentation, first-strand synthesis and second-strand synthesis steps. The libraries were sequenced on an Illumina HiSeq X10. Approximately 20 million reads of 150bp paired end data from each sample were obtained.