ITS Amplicon Sequencing of Fungal Assemblages Under Crover Cropped Soils
DNA extracted from homogenized soil samples from each cover crop treatment. Preparation for ITS sequencing was performed via PCR with the ITS1-F and ITS2 primer set to amplify DNA and add the barcodes. Sequencing was performed on an Illumina Miseq platform using paired-end sequencing (2 * 250 bp).