WGS of desert Streptomyces strain AFD10
Briefly, one microgram of genomic DNA was fragmented by covaris, the fragmented DNA is repaired, an A is ligated to the 3 end, Illumina adapters are then ligated to the fragments, and the sample is size selected aiming for a 400~500 base pair products. The size selected product is PCR amplified, and the final product is validated using the Agilent Bioanalyzer. And then we sequenced using the HiSeq 2500 platform (Illumina,San Diego, USA)