Whole exome sequencing of human leiomyoma
Whole exome sequencing libraries were prepared from 100 ng of genomic tumor DNA and DNA extracted from patient-matched normal uterine tissue or white blood cells as germline control. Tumor and germline DNA samples were sheared before library construction using Covaris S2 instrument to obtain ~170bp fragments. Libraries were prepared using the KAPA LTP Library Preparation Kit (KAPA Biosystems). Hybridization-based enrichment of specific sequences was performed using the SeqCap EZ Human Exome v3.0 Library (Roche) at 47C for 6472 hours. Enrichment following hybridization was evaluated by quantitative polymerase chain reaction (qPCR) using a Power SYBR Green PCR Master Mix (ThermoFisher Scientific)