RNA-seq of Landoltia puntata 6001: plant treated by cadmium
The samples treated with 20 ŚĚM CdCl2 were collected at at 1st day, 3rd day and 6th day respectively. Then total RNA was extracted using PLANTpure Universal RNA Kit including the untreated sample, and a post-treatment with DNase Digestion Kit removed contaminated DNA. RNA degradation and contamination was monitored on 1% agarose gels. RNA purity and concentration were measured using the NanoPhotometer? spectrophotometer and Qubit? RNA Assay Kit in Qubit? 2.0 Flurometer. RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system. A total amount of 1.5 ?g RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext? Ultra? RNA Library Prep Kit for Illumina? following manufacturerĄŻs recommendations and index codes were added to attribute sequences to each sample. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS according to the manufacturerĄŻs instructions. After cluster generation, the library preparations were sequenced on an Illumina Hiseq platform and paired-end reads were generated.