Bulk antibody heavy chain IR-Seq of human child experience malaria infection: Sample Tod8-Acu46m-PBMC-2
Antibody heavy chain mRNA were reverse transcribed using a pool of gene-specific primers against the 5 isotype constant regions ligated to a 12N molecular identifier and a sequencing adaptor. 1st PCR was carried out using the adaptor sequence and a pool of forward primers against the antibody leader regions. 2nd PCR was performed to add the full illumina sequencing adaptors. 2nd PCR products were gel purified and quantified via Agilent Tapestation before pooling for sequencing