RNA-seq EBC1 cell line : Pool Resistant 2
Total RNA was extracted using a TRIzol® Plus RNA Purification Kit (Purelink RNA Mini Kit, Ambion, Foster City, CA, USA). RNA quality and integrity (RIN) was measured with an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA) and quantified with a NanoDrop Spectrophotometer. For RNA library preparation the TruSeq RNA Library Preparation kit (Illumina, San Diego, CA, USA) was used.