RNA-seq of Agrobacterium tumefaciens actR, solvent control treated
First, rRNA was depleted by Ribo-Zero kit for bacteria, then the RNA was fragmented with Fragmentation Buffer from the NEBNext mRNA Library Prep Master Mix Set for Illumina. Subsequent library preparation steps were carried out with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina following the manufacturer's instructions for rRNA-depleted FFPE RNA.