Input_Oct4_48h
ChIPed DNA from three biological replicates were pooled for each OCT4 variant (WT, del-61, del-72, del-73, del-75 and del-79), SOX2, KLF4, MYC and Input controls. The amount of DNA used in the library preparation ranged from ~ 20  80 ng. Input DNA from each sample was pooled in equal amounts for a single library preparation. The DNA libraries were prepared using NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, Ipswich, MA) according to manufacturers instructions. Each library was uniquely barcoded using NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) (New England Biolabs). Size selection of the DNA libraries was carried out using SpeedBeads, which comprise magnetic carboxylate modified particles (Thermo Fisher Scientific). The quality of the DNA libraries was assessed using Agilent HS DNA Screen Tape (Agilent. Santa Clara, CA). The DNA libraries were then pooled by mixing an equal volume of each ChIP DNA library. The concentration of the ChIP DNA library pool was determined to be 31.6 nM by Qubit Fluorometric Quantitation.