RNA-seq of Lathyrus sativus L.:young shoots at 25 days after seeding
Total RNA was isolated from samples using the TRIzol Reagent (Invitrogen, USA) and treated with Dnase I. Prior to reverse transcription, mRNA was enriched with oligo (dT) beads and decomposed randomly into short fragments. First and second strand cDNA was synthesized successively, end reparation, 3-end single nucleotide A (Adenine) addition was performed separately and sequencing adaptors were ligated to the fragments. Finally, the fragments were enriched by PCR amplification to acquire De novo RNA-seq cDNA library. The library products were then sequenced via Illumina-HiSeq 4000 at Genergy BioTechnology Co. Ltd.