Rep-Seq of human
In-silico amplification was employed to create sequencing library. Based on the model learned from a deep sequencing dataset Warren et al 2011, We firstly generated around 200,000 TCR beta chain sequences with each simulating an independent recombination event. The in silico-generated sequence pool served as initial cDNA repertoire that could be obtained by RNA extraction and subsequence reverse transcription procedure in laboratory. We then used an in-house script to simulate PCR process characterized by amplification bias and PCR errors (5.0e-5).