ribosome display of 22 library : dmso treated
Library created by NNK saturation mutagenesis of specific regions of CDH1 gene using degenerate primers followed by T7 in vitro transcription. Library selected by ribosome display of N-terminal FLAG tag in the presence of DMSO control or PF846. Affinity-purified ribosomes pelleted through sucrose cushion, followed by mRNA library amplification by RT-PCR.