16S rRNA-seq of human gut metagenome: stool sample
DNA was extracted from stool samples, V3-V4 hypervariable region of the16S rRNA gene was amplified to generate a fragment of 460?bp using the forward primer 5'TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG and reverse primer 5'GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC (Klindworth et al., 2013).