ALD_SL_W_02_RNA_Bac: 16S rRNA analysis of bacterial communities in sediment, water and biofilms
Metagenomic RNA was extracted from sediment, biofilms and water filters. Contaminant DNA was digested, the RNA purified and reverse transcribed into cDNA. The V3-V4 region of the 16S rRNA was amplified. All samples were amplified in triplicate, purified and pooled in equal amounts. Sequences were determined using an Illumina MiSeq sequencer.