WGS of CHM13 with PacBio CCS
DNA extracted from cultured adherent cells with modified Gentra Puregene protocol (C Baker, KM Munson & EE Eichler, Univ of Wash); sheared on Megaruptor to 25kb, library constructed with SMRTbell Template Prep Kit 1.0 (PN: 1000-222-300), size selection with SageELF using the 0.75% Agarose Cassette (ELD7510) and the SageELF 1-18kb version2 Cassette Definition Protocol to generate 15kb and 20kb libraries (P Peluso, PacBio).