Illumina sequencing via hybrid selection in human colorectal cancer samples.
Target sequencing was designed with use of SureDesign software (Agilent Technologies Inc.), target enrichment and library preparation were accomplished using the HaloPlex target enrichment system (Agilent Technologies Inc.). Samples were DNA-barcoded and a pool of 29 samples was sequenced in a single run on an Illumina MiSeq platform with 2_250 bp read length.