16S rRNA bacterial composition,  Control diet Phase II, sample-40
Fecal sample collected from nursery piglet fed a standard Phase II diet (sample #40). Sample kept at -20C until microbial genomic DNA extractions. Microbial genomic DNA was extracted using repeated beating followed by Qiagen stool kit. PCR amplification of bacterial V1-V3 regions of 16S rRNA genes using 27F-519R primers. Sequencing using Illumina MiSeq2X300.