Coxiella burnetii isolated from unpasteurized cow's milk from South Carolina
Three C. burnetii isolates (CMSC1, CMCA1c1, and ESFL1) were grown axenically in Acidified Citrate Cysteine Medium 2 (ACCM2) following the methods of Omsland et al. (Omsland et al. 2011). DNA was extracted and purified using the QIAamp DNA mini kit tissue protocol (Qiagen, Valencia, CA) according to manufacturer directions.  DNA libraries were prepared for multiplexed, paired-end sequencing on the Illumina GAIIx sequencing platforms (Illumina, USA).