Sample taken upstream the Swedish WWTP (U)
The DNA extraction was performed by using the MoBio PowerSoilTM DNA Isolation Kit (Solona Beach, CA). The amplification was done using REPLI-g mini kit (Qiagen). Sequencing was done at GATC Biotech (Konstanz, Germany) using a Roche Genome Sequencer FLX Titanium. Barcode-based multiplexing where used to sequence the eight samples simultaneously.