Solexa paired end sequencing of a recombinant population from a cross between two rice cultivars
For genotyping, total genomic DNA was isolated from leaf tissues of RILs derived from a cross between Zhenshan 97 and Minghui 63 (Xing et al. 2002) using the CTAB method. DNA sequencing followed essentially the procedures as previously described (Huang et al. 2009). Briefly, each of the DNA samples was bar-coded and the indexed DNA samples from average 22 RILs were mixed into a pool with an equal molar concentration and then loaded on 2% agarose gels and separated by electrophoresis. The fragments of 200-300 bp were recovered and purified and then enriched by 18 cycles of PCR to obtain a solexa sequencing library.