miRNA-Seq in human breast milk exosomes, sample 3
RNA was extracted from human breast milk exosomes (equivalent of 0.5 mL milk) using the miRNeasy Micro Kit (Qiagen, Inc.). MicroRNA sequencing was performed at the DNA Sequencing Core at the University of Nebraska Medical Center (Omaha, USA). RNA integrity was confirmed using a Fragment Analyzer Automated CE System (Advanced Analytical Technologies, Inc.). Libraries were prepared for each sample using the NEXTflex Small RNA-Seq Kit v3 (Bioo Scientific).