H3K27ac ChIP-seq of atrial cardiomyocytes
Chromatin was isolated from atrial cardiac myocytes (HL-1 cells) following the manufacturer's manual of the ChIP-IT High Sensitivity Kit (Active Motif). 2 g of chromatin and 4 g of the antibody against H3K27ac (ab4729, Abcam) were used to perform the ChIP experiment. Library preparation was performed by using the NEBNext UltraTM DNA Library Prep Kit (NEB) according to the manufacturer's manual. Agencourt AMPure XP Beads (Beckman Coulter) were used for purification.