SMRT-Seq of Undaria
Genomic DNA was mechanically fragmented into sizes of ~20 kb using a Covaris g-tube. The PacBio SMRT BellTM sequencing library was constructed using a Template Prep Kit . After DNA damage and end repair, SMRT adaptors were ligated to generate SMRT BellTM templates. The Blue Pippin  was used to select sizes of the fragments. After a second round of DNA end repair, the SMRT BellTM templates were purified for final sequencing with the PacBio Sequel system.