16S rRNA gene V4 region of bacteria: adult male high-fat-diet (plus grape polyphenols)-fed Mus musculus (C57BL/6J ) cecal microbiota
Targeted PCR (denature at 94°C for 3 min, 35 cycles of 94°C for 45 sec, 50°C for 30 sec, and 72°C for 90 sec, with a final extension at 72°C for 10 min). Amplicons were cleaned with the Agencourt AMPure XP kit (Beckman Coulter).