TASeq of Human
Primers were designed with the  Primer3 algorithm and were synthesized from Macrogen (Seoul, Korea).  Amplicons were prepared by two-step-PCR using Illumina TruSeq adapters.  First, PCR reactions were carried out using 10 ng of initial template gDNA.  Then, 1st-amplicons were analyzed on 2% agarose gels and the bands with  expected size were isolated and purified using Mega quick-spin kit (iNtRON,  Korea). Next, 100 ng of purified 1st-amplicons were used as templates for  second-PCR, and the products were subsequently purified with the  aforementioned kit. 2nd-amplicons were quantified using Bioanalyzer 2100  (Agilent).