DNase-seq of W8 developing secondary xylem_large-fragments
Nuclei were isolated and chromatin was digested with 15 U, 25 U and 40 U DNase I for 10 min at 37C. The reaction was quenched with EDTA, DNA was extracted and run on an agarose gel. Fragment sizes of 150 - 300 bp across all three digestions were gel-purified