16SrDNA-Seq of prokaryote from plant leaf: Pelargonium graveolens
Barcoded primers 515F/806R; Illumina MiSeq 2x151bp; software package QIIME (Quantitative Insights Into Microbial Ecology toolkit; Caporaso et al. 2010). Forward and reverse sequences were merged (approximately 291 bp)  using the fastq-join tool (Aronesty, 2011), demultiplexed and quality filtered as described by (Bokulich et al., 2013).