Haemophilus parainfluenzae COPD-014-E1 O isolated from sputum of a patient with Chronic Obstructive Pulmonary Disease
Bacterial DNA was isolated with the QIAcube DNeasy Blood & Tissue Kit with the bacterial or yeast DNA with enzymatic lysis protocol (Qiagen, Germany) after pretreatment with 3 g/ml lysozyme for 30 min at 37C. A DNA library was prepared using the Illumina Nextera XT kit and protocol (Illumina, CA) and subsequently sequenced on an Illumina NextSeq platform using the 2x150 bp sequencing kit.