whole genome sequence of rattus norvegicus: adult male liver
In brief, sonicated DNA was end-repaired into dA-tailed fragments. The NEBNext adaptor (New England Biolabs, Ipswich, MA, USA) was ligated to the DNA fragments. The adaptor-ligated DNA was purified with AMPure XP beads (Beckman, Brea, CA, USA) and subsequently amplified using index and universal PCR primers provided in the NEB Multiplex Oligo Kit (New England Biolabs). The PCR products were then purified with AMPure XP beads.