WXS of Pt1: Tumor
Genomic DNA was sheared, end repaired, ligated with barcoded Illumina sequencing adapters, amplified, and size selected. Whole-exome capture was performed using an Agilent SureSelect Human All Exon 44 Mb version 2.0 bait set (Agilent Technologies). Resulting libraries were then qPCR quantified, pooled, and sequenced with 76 base-paired end reads. Pooled libraries were normalized to 2 nM and denatured using 0.2 NaOH before sequencing. Data were analysed using the Broad Picard Pipeline, which includes de-multiplexing, duplicate marking, and data aggregation.