RNAseq of Glyptonus antarticus : Thermal stress condition
Total RNA was extracted from pool of hepatopancreas of G. antarcticus. The tissues were dissolved using TRIzol® reagent following the manufacturer’s instructions. The subsequent quality control steps and library construction were performed by Acobiom (Montpellier, France). Briefly, total RNA quality was assessed using an Agilent 2100 bioanalyzer and Agilent RNA nano kit (Agilent Technologies, Santa Clara, CA). 1 µg of total RNA was used with Ribo-Zero Kit (Epicentre, Madison, Wisconsin) for an rRNA depletion step followed by DNase treatment using Baseline Zero protocol (Epicentre). RNA-Seq libraries were then prepared with the ScriptSeq v2 RNA-seq Library Preparation Kit (Epicentre). DNA samples were sequenced using HiSeq 2000 (Illumina) at Acobiom.