16S rRNA sequencing of kidney transplant recipient stool: Sample JL.145B
DNA was extracted using a bead-beater phenochloform extraction. Duplicate PCR reactions were performed for the V4-V5 hypervariable region. Illumina barcodes and adapters were ligated with the Illumina TruSeq Sample Preparation protocol. The products wee sequenced on an Illumina MiSeq Platform (Please see Westblade et al., Transpl Infect Dis 2019 Sep 10 doi 10.1111/tid.13167, PMID: 31502737).