Arima-HiC of human:GM12878
Arima-HiC was performed on 1 million crosslinked GM12878 cells in accordance with the Arima-HiC protocol, which utilizes multiple restriction enzymes to digest chromatin. Arima-HiC sequencing libraries were prepared by first shearing purified Arima-HiC proximally-ligated DNA and then size-selecting DNA fragments from 200-600bp using SPRI beads. The size-selected fragments were then enriched using Enrichment Beads (provided in Arima-HiC kit), and then converted into Illumina-compatible sequencing libraries with TruSeq LT adapters using the KAPA Hyper Prep kit.  After adapter ligation DNA was PCR amplified with Illumina primers for 4 cycles and library fragments of ~525 bp (insert plus adaptor and PCR primer sequences) were purified using SPRI beads. The purified DNA underwent standard QC (qPCR and Bioanalyzer) and sequenced on the HiSeq X following the manufacturer's protocols.