ChIP-seq of Eucalyptus grandis: Input (control) of sample W1
Developing secondary xylem tissue was finely ground in liquid nitrogen and chromatin was extracted as described by Li et al. (2014). ChIP was performed with anti-H3K4me3 using the protocol of Adli & Bernstein (2011), without ChIP DNA amplification. Illumina ChIP-seq libraries of immuno-enriched and control (input) samples were prepared and sequenced (SE50 reads; 200 bp library insert) by the Vincent L. Coates Genomics Sequencing Laboratory, University of Berkeley, CA.