H3K27me3 ChIP-seq of Aegilops tauschii: seedling leaves
Leaves from at least 10 plants were cross-linked with 2% formaldehyde and applied vacuum for 7 min 3 times at room temperature, then 2 M glycine was used to stop the cross-linking reaction. Chromatin immunoprecipitation was performed using antibodie. ChIP-seq Library construction was performed using NEBNext Ultra II DNA Library Prep Kit for Illumina according to the manufacturers instructions.