Targeted RNA-seq of 0.01% NPM1 mutA PBMCs
Targeted RNA-seq libraries were generated using a pool of target specific primers containing 12 nt unique molecular indices (UMIs) to individually tag RNA molecules of interest during reverse transcription, follwed by targeted PCR of the barcoded cDNA, and then library construction.  The primer pool used in this library targeted ABL1, WT1, PRAME, NPM1.