NGS of homo sapiens: adult skeletal muscle single fibre long-range mtDNA amplicon
Long-range PCR products were cleaned up using Agencourt AMPure XP beads then 100ng fragmented and barcoded following the Ion Xpress Plus gDNA and Amplicon Library preparation protocol (Thermo Fisher Scientific). Following quantification with the Agilent Bioanalyzer DNA High Sensitivity kit, individual libraries were pooled in equimolar concentrations. Template preparation and Ion 316 chip v2 loading were performed on the Ion Chef and chips were sequenced on the Ion PGM using the Ion PGM Hi-Q View Chef kit as per the manufacturers instructions (Thermo Fisher Scientific).