RNA seq (replicate 2) for 30 minutes of TNF treatment in Huvec cells
Total RNA was extracted from HUVECs at different times post-stimulation using Trizol (Ambion), DNase-treated, rRNA-depleted (RiboMinus kit; Epicentre), chemically fragmented into pieces of ~300 nt, and cDNA generated using random hexamers according to the TruSeq protocol before sequencing on a HiSeq2000 (Illumina)