Rbfox DKO EDL_replicate 1
Rbfox Knockout was induced in animals 7-weeks or older by feeding doxycycline-containing food (2mg/kg) for one week, as Cre expression is expressed exclusively in skeletal muscle in doxycycline-dependent manner. Mice were transferred to normal chow and a week later total RNA was isolated from muscles of control and double Rbfox knockout mice. The polyA selected RNA was further selected for 100bp paired-end sequencing using Illumina HiSeq 2500. The RNA sequencing was performed from muscles from two control and two Rbfox DKO animals.