RNA-Seq of mus musculus: adult female bone marrow hematopoietic stem cells
Libraries for DNA-Seq were prepared with KAPA Hyper DNA Prep Kit (KAPA Biosystem). The workflow consists of DNA fragmentation, end repair to generate blunt ends, A-tailing, adaptor ligation and PCR amplification. Different adaptors were used for multiplexing samples in one sequencing run. Library concentrations and quality were measured using Qubit ds DNA HS Assay kit (Life Technologies) and Agilent Tapestation (Agilent). Sequencing was performed on NovaSeq6000 S4 for pair end read 2x150 run. Data quality check was done on Illumina SAV. De-multiplexing was performed with Illumina Bcl2fastq2 v 2.17 program.