16S sequencing from uncontrolled pH mixed culture fermentations replica 1
The extracted DNA samples were sent to Macrogen Inc. (Seoul, South Korea) where 16S rRNA gene libraries were prepared using the universal primers 337F (GAC TCC TAC GGG AGG CWG CAG) and 805R (GAC TAC CAG GGT ATC TAA TC) which amplify the 3rd and 4th variable regions. The barcoded libraries were combined together and sequenced using Illumina MiSeq (Control Software v2.2).