Solexa sequencing of Sordaria macrospora genomic DNA, 300 bp library
To construct libraries of two different insert sizes, 5 µg DNA each were sonicated with a  Branson sonicator. Sonicated DNA was separated through 2 % NuSieve agarose gels and  fragments of ~300 bp were purified. After generation of blunt-end fragments, Aoverhangs  were added, adaptors ligated, and the fragments were PCR amplified.