ITS2 sequence diversity of a a 19 year-old post-coal mining reclamation area (ReclZ-19), replicate 1
The fungal ITS2 sequence library was constructed by following Illumina MiSeq protocol. Briefly, the protocol included a first stage amplicon PCR step involving 0.2 M concentrations of primers ITS3mix (forward) and ITS4 (reverse). Sequences were demultiplexed and depleted of Illumina barcodes and primers by using MiSeq reporter software