Illumina RNA Sequencing of Efm 410 after exposure to 1X MIC chlorhexidine, trial 2
Efm 410 was treated with 0X chlorhexidine for 15 minutes prior to RNA harvesting. RNA samples were submitted to Tufts University Core Facility. Library preparation for Illumina HiSeq 2000 sequencing was performed using Illumina TruSeq Stranded RNA Sample Preparation kit with RiboZero treatment for rRNA removal. 50 base reads were obtained from single-end sequencing.