RNA-seq of vitis:4
The enriched mRNA was obtained by using the oligo (dT) magnetic beads. then fragmented by 94 for 8 min, cDNA was synthesized by SuperscriptIII Reverse Transcriptase, followed by purification, end repairing and dA-tailing, ligated with the sequencing adap, afterwards, the PCR amplification were conducted by indexed primers.