16S  rRNA amplicon sequencing of Homo Sapiens: swab from healthy volunteer
Construction  of the 16S rRNA amplicon library was based on the dual-index strategy,  multiplexing 8 forward and 12 reverse primers. PCR amplification primers  targeting the V3-V4 variable regions of the 16S rRNA gene were designed to  contain a 58-bp adapter sequence specific for the Illumina MiSeq platform at  the 5, a 3 to 6-bp variability region, a 12-bp Golay barcode, and a 16S  rRNA-gene specific primer sequence universal for Prokaryotes.