RNA-Seq of Pishtaz cv. grains at 21 day post anthesis
Grain sampling was done at 21 day post anthesis (DPA). Total RNA of ten         grains of each spike was isolated by TRIzol Reagent (Invitrogen) and the extracted RNAs of         10 spikes were pooled to obtain the final RNA sample for sequencing. mRNA capturing from         total RNA was done using PolyA approach and RNA sequencing was conducted as paired-end with         150nt length using Illumina HiSeq 2500.