RNA-seq of a salt tolerant peanut A025
The seeds of the drought-resistant cultivar Fenghua3 were incubated in pots with a mixture of vermiculite, perlite and soil (1: 1: 1) at 26 ℃ with photoperiod of 16 h. Seedlings were then grown in an artificial climate incubater for 14 days under restrained conditions (16 h light 26 ℃ / 8 h dark 22 ℃ cycles). At the three-leaf stage, the robust overground parts and more uniform growing seedlings were harvested as control. The parts of these plants were then grown in salinity stress conditions with Hoagland's solution and 200 mM (1%) NaCl. The robust growing seedlings were harvested at 6, 12, 18, 24, and 48 hours later. Young leaves of peanut seedlings growing within salinity stress conditions for 12 h were used to perform transcriptome analysis. Total RNA was extracted from 20 mg young leaves using GeneJET Plant RNA Purification Mini Kit (Thermo Fisher Scientific, Waltham, Massachusetts, USA), and quantified by NanoDrop ND-2000 (Thermo Fisher Scientific, Waltham, Massachusetts, USA). RNA integrity was assessed using Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, California, USA). The samples with integrity number greater than 8 was used for libraries construction. The library of each sample was constructed using the TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA, USA) and SuperScript II Reverse Transcriptase (Invitrogen, Carlsbad, CA, USA). Paired-end sequencing was performed using an Illumina sequencing HiSeq 4000 platform with PE-150 module (Illumina, San Diego, CA, USA).