Molecular Architecture of the Microbial Community of Peltigera Lichen
Total DNA was extracted from lichen samples as follows: the surface of the lichen sample (1cm x 1cm) was carefully washed 3 times in a sterile tube with 1 mL of nuclease free and sterile water. After the final wash, the lichen sample was transferred to a clean microcentrifuge tube provided in the Power soil DNA extraction kit (MO BIO Laboratories, Inc., Carlsbad, CA). DNA was extracted according to manufacturer’s instructions. DNA concentration was determined by using the Qubit dsDNA dBR assay kit (Thermofisher Scientific, San Diego, CA). A paired-end sequencing library was prepared using the Nextera XT DNA library preparation kit (Illumina, San Diego, CA), and sequencing was performed by University of California San Diego Sequencing core by using an Illumina MiSeq platform (Illumina) (250 bp paired-end reads). Paired-end sequence data was downloaded from the basespace application (Illumina, San Diego).