Bacillus thuringiensis S3580-1, whole genome shotgun sequence
Random interrupted genomic DNA to 400-600 bp, and at the end of filling in the 3 'end with A base, then connect the pair-end connector of Illumina. The fragments were subjected to agarose gel electrophoresis, and the 500-700 bp range was recovered by gel cutting. The recovered bands were amplified by 10 cycles of PCR and the pair-end Library of the 500 bp insert was obtained.