Biofilm sample
A two-stage PCR protocol (Naqib et al. 2018) was used to amplify                 fragments of microbial DNA and employed a primer set targeting the V3-V4 variable                 region of microbial 16S rRNA genes. The amplified fragments were sequenced on an                 Illumina sequencer, employing paired-end 2x255 base sequencing reads.Reference:                 Naqib A., Poggi S., Wang W., Hyde M., Kunstman K., Green S.J. (2018) Making and                 Sequencing Heavily Multiplexed, High-Throughput 16S Ribosomal RNA Gene Amplicon                 Libraries Using a Flexible, Two-Stage PCR Protocol. In: Raghavachari N.,                 Garcia-Reyero N. (eds) Gene Expression Analysis. Methods in Molecular Biology, vol                 1783. Humana Press, New York, NY