Oobius  primorskyensis genomic sequences
Oobius primorskyensis from the Russian Far East (near Vladivostok) were reared using freshly laid eggs of A. planipennis on coffee filter paper at the BIIRU quarantine laboratories (USDA-ARS, Newark, Delaware) for 3 – 10 generations before DNA was extracted.  For each species, 30 – 50 adult parasitoids were killed in molecular grade ethanol, their DNA extracted with the Qiagen DNeasy Blood and Tissue Kit (Qiagen, Valencia, CA), then quantified with the Quant-iT PicoGreen dsDNA Kit (Invitrogen, Grand Island, NY).  DNA from each species was used to make genomic libraries with the standard Illumina TruSeq DNA Sample Preparation Kit for paired-end libraries.  The libraries were sequenced as 150-nucleotide, paired-end, reads on an Illumina HiSeq 2500 at the Delaware Biotechnology Institute, Newark, Delaware, which produced 109 million reads that included 16 gigabases of DNA sequence for each species.