WGS of Mycobacterium avium subsp. paratuberculosis: IS1311 type S IS900 RFLP type S1 strain
Genomic DNA was prepared by isopropanol precipitation and a 70% ethanol wash of CTAB/phenol/chloroform extracted cellular material after stepwise enzymatic digestion with lysozyme, RNase A, and proteinase K.  The DNA was also digested with mutanolysin prior to proteinase K digestion, and subjected to an extra clean-up and concentration on Ampure PB magnetic beads. A PacBio library was constructed using P6-C4 chemistry with 8-30 kbp fragments.