Mate-pair reads from 8 to 10 kb insert sizes
The genomic DNA was extracted as follows: 100 mg of wasps were ground into a fine powder in liquid nitrogen and kept for lysis at 55C in SNET buffer (400 mM NaCl, 1% SDS, 20 mM Tris-HCl pH8.0, 5 mM EDTA pH 8.0 and 2 mg/ml Proteinase K) with gentle rotation at 10 rpm overnight. Next day, after RNase A (100 g/ml) digestion, Phenol:Chloroform:Isoamyl Alcohol extraction was performed followed by Ethanol precipitation. The pellet was resuspended in 1X Tris-EDTA buffer (pH 8.0).