Raw sequencing data from pooled fitness assay of Saccharomyces cerevisiae double CRISPRi strains
DNA was isolated using the YeaStar Genomic DNA Kit (Zymo Research). PCR to amplify the CRISPRiSeq guide locus was performed across 4 tubes on a total of 150ng DNA using Q5 polymerase (NEB) using 22 cycles and custom oligos designed to add multiplexing tags and Illumina adaptors. Reactions were purified with PCR purification columns (Qiagen) and purified again on E-Gel SizeSelect 2% Agarose Gels (Invitrogen).