RNA-seq of Phlox drummondii: adult dark-red plant
We sampled five tissues from one individual of each species: flower bud, mature flower, leaf, stem, and root. RNA was extracted using E.Z.N.A.¨ Plant RNA Kit (Omega Bio-tek¨), including three additional washes with 70% ethanol before membrane elution. Equimolar amounts of RNA from the different tissues of an individual were pooled. The four pools were prepared using Apollo Poly-A isolation and mRNA directional library preparation, and then 2x125 bp paired-end sequences were obtained using an Illumina HiSeq 2500. Sequencing and library preparation was conducted at the Bauer Core of Harvard University.