Subgingival plaque of tooth 11 from periodontally healthy subject 05 (shallow site)
Metagenomic DNA extracted from subgingival plaque was used as template for PCR amplification using primers targeted to the V4 region of 16S bacterial rDNA as described by Kozich et al., 2013, Appl Environ Microbiol. 2013 Sep;79(17):5112–5120