Transcriptome profiles of Populus to bacterial pathogen infection
mRNA was enriched using Oligo d(T) magnetic beads and then mixed with fragmentation buffer, and was fragmented into short fragments (~200 nt). cDNA was synthesized using random hexamer primer with the mRNA fragments as templates. Double-stranded cDNA was purified with magnetic beads. End reparation and 3'-end single nucleotide A (adenine) addition was then performed. Finally, sequencing adaptors were ligated to the fragments. PCR was performed to selectively enrich and amplify the fragments. The cDNA library was sequenced on a flow cell using an Illumina HiSeqTM 2000