GSM2114055: PREM35; Homo sapiens; RNA-Seq
60-100 mg of frozen tissues per sample, which had been treated with RNAlater® (Sigma) overnight at 2-4oC prior to -80oC storage, were mechanically homogenised in TRIzol® (Life Technologies), using a Precellys 24 bead-based homogeniser, and total RNA was extracted from the aqueous phase of chloroform-treated and centrifuged homogenates using the TRIzol® Plus RNA Purification kit (Life Technologies) with on-column DNase treatment prior to elution with RNase-free water. Sample libraries were made using the TruSeq Stranded mRNA Sample Preparation kit, following the high-throughput sample (HT) protocol (Illumina); quality assessment of RNA samples and their cDNA libraries were assessed using a Life Technologies Qubit fluorimeter and Agilent Technologies Bioanalyser 2100
GEO Accession
GSM2114055