RGT-root
After the total RNA sample is qualified, the mRNA is enriched with magnetic beads with Oligo (dT), and a fragment buffer is added to the obtained mRNA to make the fragment into a short fragment. The fragmented mRNA is used as a template and six bases are used. Random primers were used to synthesize the first strand of cDNA, and buffers, dNTPs, RNaseH, and DNA Polymerase I were added to continue to synthesize the second strand of cDNA, purified by QIAQuickPCR kit and eluted with EB buffer. The purified double-stranded cDNA was eluted and then subjected to end repair, base A, and sequencing adapter treatment, and then the target size fragments were recovered by agarose gel electrophoresis and PCR amplified to complete the entire library preparation.