Whole genome bisulfite sequencing of Homo Sapiens: HUES8 human embryonic stem cells WT
Genomic DNA was isolated from HUES8 WT and HUES8 TKO hESCs. Genomic DNA was sheared using E220 focused-ultrasonicator to 250-350bp fragments. After end-repair and TruSeq adapter (Illumina) ligation the DNA-libraries were denatured followed by treatment with bisulfite.