the goal of the study was to investigate transcriptome variations                     associated with Bti resistance in mosquito larvae. Two different strains of the                     mosquito Aedes aegypti were compared: One susceptible to all insecticides and                     another selected for more than 20 generations with Bti toxines. Calibrated                     fourth stage larvae of both strains were grown in standard laboratory conditions                     and then immediately used for total RNA isolation. Three independant biological                     replicates were obtained for each sample. For each sample, total RNAs from each                     biological replicates were pooled in equal proportion. For each sample, pool of                     total RNAs was used for the preparation of digital gene expression tag profiling                     (DGETP) cDNA library using DpnII restriction enzyme as described by                     illumina