Nanopore DNA-seq of Bacteroides fragilis: BF17. Second run. demultiplexed fast5.
DNA extraction with Qiagen Genomic Tip g/500.  Library prep: Rapid Barcoding kit (SQK-RPB004) (protocol version RPB_9059_v1_revC_08Mar2018): input DNA 400 ng. This sample: BARCODE 02. (Multiplex of four barcodes: 01,02,03,04). AmPure XP cleanup. FLO-MIN106. MinKNOW/2.2.12. Demultiplexed with deepbinner v0.2.0 (https://github.com/rrwick/Deepbinner) and basecalled using Albacore v2.3.3, retaining only barcodes that deepbinner and Albacore agreed on for minimal barcode misclassification. Adapters and barcodes have not been trimmed from the fast5 data. Run ID: bf17_s01_99377