GSM2114161: RatID_5_CX_SHM_mRNA; Rattus norvegicus; RNA-Seq
Tissue was collected at 3 months after TBI. For tissue collection rats were anesthetized with isoflurane and decapitated. The brain was removed from skull, flushed with 0.9% cold sodium chloride, and two 2-mm-thick coronal slices (between -2.2 to -6.2 from the bregma) were cut with a slicing matrix. Perilesional cortex, thalamus, and dentate gyrus (including CA3c-b) were dissected under the magnifying glass on top of the light table.  Samples were stored at -80oC until RNA extraction. Total RNA extraction were done by using the DNaesy Blood & Tissue kit (Qiagen #69504), followed by DNase digestion. RNA quality was verified on the Shimadzu MultiNA capillary electrophoresis system (Shimadzu). Poly-A mRNA was isolated with Dynabead Oligo(dT) enrichment (Invitrogen). Sequencing libraries were prepared using the NEBNext® mRNA Library Prep Reagent Set for Illumina® (New England Biolabs). Libraries were quantified on MultiNA system and sequenced at a concentration of 13 pM on the Genome Analyzer IIx (Illumina Inc, CA, USA) using the TruSeq version 5 cluster kit (cBot) and SBS kit (36 cycles).
GEO Accession
GSM2114161