GSM4160547: ATAC-seq_Adult_Brain_GFPpos_R2; Mus musculus; ATAC-seq
For ATAC-seq, ~50,000 GFP-positive FACS-sorted cells or trypsinized cultured ECs were gently centrifuged and then resuspended in ice-cold lysis buffer (0.25 M sucrose, 25 mM KCl, 5 mM MgCl2, 20 mM Tricine-KOH, 0.1% Igepal CA-630) and immediately centrifuged at 500 x g for 10 min at 4°C to prepare nuclei. The resulting nuclear pellet was resuspended in a 50 ul reaction volume in Tn5 transposase and transposase reaction buffer (FC-121–1030, Illumina Inc, San Diego, CA), and the tagmentation reaction was incubated at 37°C for 30 min. Tagmented DNA was purified using QIAGEN MinElute Gel Extraction kit (28604, Qiagen). ATAC-seq libraries were PCR amplified for 11 cycles. Agencourt AMPure XP beads (A63880, Beckman Coulter) were used to purify ATAC-seq libraries, which were then paired-end sequenced for 36 cycles on a NextSeq500 (Illumina).
GEO Accession
GSM4160547