GSM2730922: mTEC WT 1; Mus musculus; RNA-Seq
Cell pellets were snap frozen on dry ice and and stored at -80°C, then RNA was isolated with miRNeasy Micro Kit (Qiagen) First strand cDNA synthesis and cDNA amplification was performed using the SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (Clontech Laboratories) according to manufacturer’s instructions. Complementary DNA libraries were prepared and indexed separately using the Nextera® XT DNA Library Preparation Kit (Illumina) following manufacturer’s instructions. Each indexed sample library was quantified using the Agilent Tapestation and the Qubit™ DNA BR assay kit for Qubit 3.0® Fluorometer (Life technologies). The indexed sample libraries were pooled and diluted to 1.5pM for 75 base paired-end sequencing on a a NextSeq 500 instrument using the v2 150 cycle High Output kit (illumina) as per manufacturer’s instructions.
GEO Accession
GSM2730922