M9261
N. meningitidis genomes were characterized via 454 pyrosequencing  (Margulies, et al., 2005) using either a half or one quarter plate  runs on the Roche 454 GS-20 or GS Titanium instrument (Table  1). For each genome, a random shotgun library was produced using  Roche protocols for nebulization, end-polishing, adaptor ligation,  nick repair and single-stranded library formation. Following emulsion  PCR, DNA bound beads were isolated and sequenced using  long read (LR) sequencing kits. The number of reads produced in  the experiments ranged from 200,000 to 600,000, and the average  read lengths were between 100 and 330 bases. These data yielded  47.6-94.3 million bases per genome amounting to 20-40x coverage  for the approx. 2.1 megabase N. meningitidis genomes. After read  trimming and re-filtering to recover short quality reads, the data  were passed to the first stage of the pipeline – genome assembly.