GSM3466898: Lin-Kit+ Bone Marrow; Mus musculus; RNA-Seq
Fresh bone marrow cells were isolated by crushing mouse bones in media supplemented with FCS. Bone chips were enzymetically digested using collagenase I and dispase. Cells were stained with fluorescently labelled antibodies against lineage markers (CD4, CD8, CD11b, B220, Gr1, Ter119), CD71 and CD45. A DNA dye was used to exclude debris. Single Lin-CD45-CD71-DNA+ cells were sorted into lysis buffer. Full-length cDNA from individual cells were produced following the Chromium Single Cell 3' Reagent v2 protocol (10x genomics, Pleasanton, CA) according to manufacturer's recommendations. Default library production protocol, 10x genomics, Pleasanton, CA
GEO Accession
GSM3466898