GSM3374535: 4TO7 EVs; Mus musculus; miRNA-Seq
RNAs were extracted using Trizol. Ribosomal RNAs were removed using a Ribozero kit. RNAs were separated by denaturing polyacrylamide gel electrophoresis and stained with SYBR-Gold (Invitrogen). miRNA-sized small RNAs were excised from the polyacrylamide gel. Small RNAs were eluted in 0.3 M NaCl from crushed gel pieces. After recovery, precipitation and washing, small RNAs were suspended in nuclease-free water. The RNAs were converted to cDNAs using a NEBNext® small RNA library prep kit (New England Biolabs, USA) according to the manufacturer's instructions.
GEO Accession
GSM3374535