Capture enriched library of  etDNA of 10 invertebrate species mock community number 2
DNA was sheared into approximately  600 nucleotide fragments by ultrasound sonication. Library preparation was  conducted using NEBNext UltraTM II DNA Library Prep Kit for Illumina (New  England BioLabs, USA) following manufacturer's instructions. COI capture  enrichment was conducted using myBaits Custom kit following manufacturer's  instructions (Arbor Biosciences, USA). Baits were diluted 10 times and  hybridization lasted 24 h for both type of DNA. The final library  amplification was performed with the KAPA HiFi DNA Polymerase (Kapa  Biosystems, USA) and reampP5 and reampP7 Illumina primers. Capture library  concentrations were determined by qPCR with a KAPA qPCR kit (KAPA Library  Quant Kit, Kapa Biosystems, USA).