GSM3466330: NSUN2-KO HEK293 RNA-Bisulfite-Seq; Homo sapiens; OTHER
Total RNA of cells was extracted using TRIzol according to the manufacturer's instructions. Total RNA for each sample was rRNA depleted using NEBNext® rRNA Depletion Kit. rRNA-depleted RNA was bisulfite-converted and purified using the EZ RNA methylation Kit (Zymo Research). RNA libraries were later constructed with TruSeq Stranded Total RNA Library Prep Kit (Illumina) according to the manufacturer's instructions.
GEO Accession
GSM3466330