GSM4314785: BLU9931 treated and released 2; Homo sapiens; RNA-Seq
Single cell suspensions were obtained using using roto-stator homogenization and miltenyi tumor dissociation kits (130-096-730). Single cell suspensions were input to a 10x Genomics Chromium machine to establish single-cell gel beads in emulsion (GEMs) for directed retrieval of approximately 8000-10000 cells Single-cell RNA-Seq libraries were primed using the following Single Cell 3' Reagent Kits v3: Chromium™ Single Cell 3' Library & Gel Bead Kit v3, PN-1000092; Protocols were performed as directed in the Single Cell 3' Reagent Kits v3 User Guide (# CG000183 Rev B) Libraries were sequenced on an Illumina NextSeq500 in the paired-end format 28x91x8 (R1xR2xI)
GEO Accession
GSM4314785