WGA of Cattle: liquid on the yak brain membrane
The DNA sample that passed the electrophoresis test was broken with Covaris g-TUBE into the desired fragment of the desired size of the library. After DNA damage repair and end repair, the hairpin type adaptor was ligated to both ends of the DNA fragment using DNA adhesive enzyme, using AMpure. Purification of DNA fragments by PB magnetic beads to construct SMRT Bell library