RNA-Seq of Aspergillus niger EA21.6
Samples grown to mid-exponential phase were used to isolate RNA using TRIzol® reagent (Invitrogen) and purified with NucleoSpin RNA Clean-up kit (Macherey-Nagel) with rDNase treatment. RNA quantity and quality of the samples was checked with a NanoDrop-2000 spectrophotometer and by RNA gel electrophoresis, respectively. RNA sequencing was conducted by Genome scan (Leiden, the Netherlands). mRNA was isolated from the total RNA using NEBNext Ultra Directional RNA Library Prep Kit for Illumina according to the protocol. After fragmentation of the mRNA, cDNA was synthesized for ligation with the sequencing adapters and PCR amplification. Clustering and DNA sequencing was performed using the Illumina NextSeq 500 according to the manufacturer’s protocols.