GSM3674428: PAO1-5; Pseudomonas aeruginosa PAO1; RNA-Seq
To examine the effect of TFs (taking an example of RpoN) on the transcriptome, 2 ml of mid-log-phase (OD600 = 0.6) bacterial cultures (PAO1 and rpoN::Tc mutant strains) were collected by centrifugation (12,000 rpm, 4℃). An RNeasy minikit (Qiagen) was usedr subsequent RNA purification with DNaseI (NEB) treatment. After removing rRNA by using the MICROBExpress kit (Ambion), mRNA was used to generate the cDNA library according to the NEBNext® UltraTM II RNA Library Prep Kit protocol (Illumina), which was then sequenced using the Illumina HiSeq 2000. Each sample in RNA-seq assay was repeated twice. TruSeq RNA sample prep kit protocol (Illumina)
GEO Accession
GSM3674428