GSM1234287: m_ehb; Apis mellifera; Bisulfite-Seq
Bisulfite: Using guidelines from Illumina (Whole‐genome Bisulfite Sequencing for Methylation Analysis, Part # 15021861 Rev. B). Fragment the gDNA to 250 bp peak size; convert overhangs resulting from fragmentation into blunt ends using T4 DNA polymerase and Klenow enzyme; add a single ‘A’ nucleotide to the 3’ end; ligates adapters to the ends of the DNA fragments; remove unligated adapters, and select a 275–350 bp size‐range of DNA fragments for bisulfite treatment; treat with bisulfite to convert any un‐methylated Cytosine to Thymidine (EpiTect Bisulfite Kit); PCR to selectively enrich those DNA fragments that have methylated adapter molecules on both ends
GEO Accession
GSM1234287