GSM2461860: LOS #4 WGBS; Bos indicus x Bos taurus; Bisulfite-Seq
DNA were isolated using the phenol-chrolorform method For whole genome bisulfite sequencing (WGBS) library preparation, 1μg bovine DNA was subject to sodium bisulfite mutagenesis using NEB EpiMark Bisulfite Conversion Kit (NEB E3318) according to the manufacturer's instructions.  Prior to the sodium bisulfite reaction, 5ng (0.5%) unmethylated cl857 Sam7 Lambda DNA (48,502bp, Promega D1521) was combined with bovine DNA to act as an internal control to monitor the bisulfite conversion rate. The WGBS libraries were generated using the NEBNext Ultra DNA library Prep Kit for Illumina (NEB E7370) as per the manufacturer's instructions.
GEO Accession
GSM2461860