Illumina HiSeq 2000 sequencing; microRNAseq of primary human gallbladder cells, reprogrammed human gallbladder cells, and human pancreatic beta cells
microRNAseq of primary human gallbladder cells, reprogrammed human gallbladder cells, and human pancreatic beta cells
In vitro culture protocol for human gallbladder cells. Dissociated cells from human gallbladder and cystic duct were cultured in rat mammary feeder culture using a modified culture medium as published in PMID 23562832. Isolation of RNA from gallbladder cells and beta cells using Qiagen RNeasy protocol microRNA libraries were generated and processed by the University of Pennsylvania’s Next Generation Sequencing Core using Illumina truSeq small RNA library preparation kit (Illumina, San Diego, CA, USA)
Experimental Factor: gallbladder cell
cell type
Experimental Factor: no enrichment
phenotype