GSM4484343: WT_ATAC-seq_rep1; Mus musculus; ATAC-seq
Tibialis anterior (TA) muscle were dissected from mice and flash frozen in liquid nitrogen. After thawing of the sample, muscle were minced with scissors in a buffer containing 2,15M sucrose following by an ultraturrax treatment to release nuclei. After ultracentrifugation nuclei are washed to removed the sucrose and a small fraction is counted with DAPI under a microscope. 50 000 nuclei are used to perform the ATAC-Seq library according to Buenrostro et al 2013 (Nat Methods. 2013 Dec;10(12):1213-8)