Liuye50-heat treatment 12 h
The cDNA libraries were constructed using Illumina TruSeq Small RNA Preparation Kit following the manufacturers protocol. In brief, RNA 3- and RNA 5-adapters were ligated to total RNA, cDNA constructs were created using reverse transcription after PCR, and then samples of small cDNA fragments of different lengths (1830 nt) were run on 6% denaturing polyacrylamide gel by electrophoresis. The final cDNA libraries were sequenced using Illumina HiSeq at the Beijing Genomics Institute (BGI, Shenzhen, China).