Incomplete recovery and individualized responses of the human distal gut microbiota to repeated antibiotic perturbation - Subject D partial
Proximal spacer:GG;   Proximal primers (533-515 reverse): TACCGCGGCTGCTGGCAC, TACCGCGGCGGCTGGCAC; Proximal spacer + primer variants accepted for quality filter (reads lacking an expected variant at the proximal end were rejected):  GGTACCGCGGCTGCTGGCAC, GGTACCGCGGCGGCTGGCAC, GTACCGCGGCTGCTGGCAC, GTACCGCGGCGGCTGGCAC, GGGTACCGCGGCTGCTGGCAC, GGGTACCGCGGCGGCTGGCAC; Distal spacer: CA;  Distal primers(8-27 forward): AGAGTTTGATCATGGCTCAG, AGAGTTTGATCCTGGCTCAG, AGAGTTTGATTCTGGCTCAG, AGGGTTCGATTCTGGCTCAG; Distal spacer + primer recognition not required for binning/trimming/filtering.       Sample/Barcode (a' and 'b' suffix indicate replicate PCR reactions under identical conditions): D1/ACGAGTGCGT;  D2/ACGCTCGACA;  D3/AGACGCACTC;  D4/AGCACTGTAG;  D5/ATCAGACACG;  D8/ATATCGCGAG;  D11_a/CGTGTCTCTA (replicate PCR identical conditions); D11_b/CTCGCGTGTC (replicate PCR identical conditions); D12/TAGTATCAGC;  D14/TCTCTATGCG;  D16_a/TGATACGTCT (replicate PCR identical conditions); D16_b/TACTGAGCTA (replicate PCR identical conditions); D17/CATAGTAGTG;  D18/CGAGAGATAC;  D20/ATACGACGTA;  D23/TCACGTACTA;  D24/CGTCTAGTAC;  D25/TCTACGTAGC;  D26/TGTACTACTC;  D27/ACGACTACAG;  D28/CGTAGACTAG;  D29/TACGAGTATG;  D30/TACTCTCGTG;  D31/TAGAGACGAG;  D32/TCGTCGCTCG;  D35/ACATACGCGT;  D38/ACGCGAGTAT;  D39/ACTACTATGT;  D41/ACTGTACAGT;  D43/AGACTATACT;  D44/AGCGTCGTCT;  D45/AGTACGCTAT;  D47/ATAGAGTACT;  D50/CACGCTACGT;  D51/CAGTAGACGT;  D52/CGACGTGACT;  D53/TACACACACT;  D54/TACACGTGAT;  D55/TACAGATCGT;  D56/TACGCTGTCT;  