GSM3467063: ABA treatment rep2; Zea mays; miRNA-Seq
TRIzol reagent (Invitrogen) was used to isolate total RNA, and then small RNAs were enriched using polyethylene glycol (PEG8000). For small RNA library construction, enriched small RNAs were ligated with 3' RNA adapter, and fractionated through 15% denaturing polyacrylamide gel electrophoresis (PAGE). Products with a length of 36 to 44 nucleotides (nt) were ligated with 5' RNA adapter, and used to perform RT-PCR amplification. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM3467063