EdUseq-HU/release of U2OS cells expressing normal levels of cyclin E: 14 h HU arrest and release for 60 min, treated with DRB for the first 7 h after mitotic exit, EdU was added for the last 30 min of release  (replicate 2)
U2OS cells expressing normal levels of cyclin E were synchronized by mitotic shake-off and released into HU-containing media for 14 h, cells were then released from HU for 60 minutes with EdU being added 30 minutes before collection. DRB was present for the first 7 h after mitotic exit. The EdU labeled DNA was then isolated and sequenced.