GSM4271124: 1k_CART_cells; Homo sapiens; ATAC-seq
T cells were thawed and checked for purity based on cell-surface expression of CD3, CD4, CD8 and CAR via flow cytometry. For scATAC-seq experiments, the first experiment on ~5000 CD8+CAR+ T cells  were isolated using bead-based positive selection, according to the manufacturer's instructions (Miltenyi Biotech), while in the second experiment on ~1000 cells, they were FACS sorted with CD8 and CAR. scATAC-seq was carried out using the Chromium platform and following the standard protocols provided by 10X Genomics: 1) “Chromium Single Cell ATAC Reagent Kits” and 2) “Nuclei Isolation for Single Cell ATAC Sequencing.” Libraries were paired-end sequenced (50 bp + 50 bp) on a NextSeq 550 (Illumina). scATAC-seq
GEO Accession
GSM4271124