GSM3905173: Brain_S_SHAM_Air_2; Rattus norvegicus; RNA-Seq
Brainstem and hypothalamus were removed, flash frozen on dry ice, and RNA was harvested Direct-Zol RNA MiniPrep kit from Zymo Research (Irvine, CA, USA). Total mRNA was isolated  (Wafergen Biosystems, Fremont, CA, USA) and run in Apollo324 automated sample processing system for mRNA selection. RNA-Seq library was prepared with Wafergen's PrepX mRNA 48 Protocol. cDNA libraries were amplified and dsDNA from each library were quality checked by Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Each library was sequenced according to Illumina NextSeq 500 protocols (1.8 pM + 5% PhiX from Illumina Inc., San Diego, CA, USA) RNA libraries were prepared for sequencing using standard Illumina protocols