GSM4160224: Small RNA-Seq: HN1: Uninfected Normal Liver Tissue; Homo sapiens; miRNA-Seq
About 5 mg of liver tissues were powdered keeping frozen in liquid nitrogen and the total RNA was isolated using TRIzol (Invitrogen, CA) following the manufacturer's protocol. Quality of each RNA was verified using Bioanalyzer (Agilent, CA) and RNA integrity number (RIN) ≥6.5 was considered for Next Generation Sequencing (NGS). Total RNA isolated from liver tissues were subjected to high throughput small RNA sequence analysis using Illumina HiSeq2500 in Medgenome Labs Private Limited, Bangalore, India. In brief, total RNA was ligated to 3'and 5' adaptors, followed by reverse transcription and amplification using Truseq Small RNA Sample Prep Kit (Illumina, USA). The RNA fragment of length 145-160nt was gel purified to enrich small RNA library and sequenced.
GEO Accession
GSM4160224