GSM3374752: D21_input genomic DNA; Mus musculus; OTHER
Nucleus preparations and HRS assays were performed as previously described in (Milligan et al. 2000 Oncogene 19, 5810-16) and (Braem et al. 2008 J. Biol. Chem. 283, 18612-20). For each sequencing library, 150 ng of genomic DNA issued from the HRS or loop fractions of the assays were used. Briefly, a first biotinylated StyI DNA adaptor, containing a binding site for the MmeI type IIS restriction enzyme, is ligated to the StyI fragments. Ligated products are then captured onto streptavidin beads and digested with MmeI to homogenize the size of the StyI restriction fragments (~18 to 20 nucleotides). A second sequencing adaptor is ligated to MmeI restricted sites and DNA fragments are released from streptavidin beads, purified on an acrylamide gel and sequenced using high-throughput sequencing.
GEO Accession
GSM3374752