PacBio RSII WGS of Hawaiian Crow
Phenol-chloroform used to extract high molecular weight genomic DNA from blood sample taken of single male. DNA sheared to target 50 kb fragments with Megaruptor (Diagenode). Sheared DNA constructed into SMRTbell template using > 30 kb library construction protocol and 20-kb size selection with BluePippin system (Sage Science). Sequence data generated with PacBio RSII instrument with P6v2 polymerase binding and C4 chemistry kits (P6-C4) and 6-hour run time movies.