GSM4409548: Col-0_gp6_IPARE_rep5; Arabidopsis thaliana; RIP-Seq
Immunoprecipitation followed by Analysis of RNA Ends (IPARE) was performed as described in the associated publication. Briefly, RNA was immunoprecipitated using an anti-NRPE1 antibody and the high throughput sequencing library was prepared using a modified template switching NanoPARE protocol. WGBS samples: Genomic DNA was extracted from 3.5-week old Arabidopsis leaves using Dneasy Plant Mini Kit. Library was prepared by a modified version of NanoPARE protocol (PMID: 30355603). RNA was polyadenylated with Poly(A) polymerase. The polyadenylated RNA was EtOH-precepitated. The deadenylated RNA was DNase treated with TURBO DNase. The RNA was EtOH precepitated. The RT, template switching, pre-amplification and 2nd PCR were performed as NanoPARE. The DNA extracted for WGBS were processed with bisulfite treatment followed by 100 bp paired-end sequencing.
GEO Accession
GSM4409548