GSM4160463: NPC.CL31.Aux; Mus musculus; Hi-C
Hi-C was performed as previously described (Belaghzal et al., 2017), except that ligated DNA size selection was omitted, and dA-tailing was performed prior to biotin pull-down. Briefly, each Hi-C experiment was performed on 10 million cells (NPCs) per sample. Cells were digested with DpnII at 37°C overnight. DNA ends were filled with biotin-14-dATP at 23°C for 4 hours. DNA was then ligated with T4 DNA ligase at 16°C overnight. Binding proteins were removed by treating ligated DNA with proteinase K at 65°C overnight. Purified proximally ligated molecules were fragmented to obtain an average fragment size of 200 bp. After DNA end repair, dA-tailing and biotin enrichment, DNA molecules were ligated to Illumina TruSeq sequencing adapters at room temperature for 2h. Final library PCR productions were carried out following the Illumina TruSeq Nano DNA Sample Prep Kit manual.
GEO Accession
GSM4160463