GSM4409488: BVDV-UUUGUAG/let-7; Bos taurus; RIP-Seq
UV crosslinked cells were lysed in 1x PXL buffer, lysates were Dnase treated, partially RnaseA digested, clarified by centrifugation, and Argonaute-RNA complexes isolated with pan-Ago 2A8 antibody Libraries were prepared as in Moore et al. 2014 Nature Protocols, but with the following modifications: A 2nd PCR step was used to introduce library specific indices that were additionally compatible with MiSeq sequencers using version2 chemistry.
GEO Accession
GSM4409488