RNA-Seq of Nemopilema nomurai tentacles
poly(A) mRNA was isolated using Oligo (dT) beads and interrupted to short fragments. These fragments were then transcribed into first-strand cDNA, followed by synthesis of the second strand. The synthesized cDNA products were purified using a QiaQuick PCR extraction kit (Qiagen, Valencia, CA, USA) and dissolved in EB buffer for end repair and poly(A) addition. Subsequently, the cDNA fragments were ligated to the sequencing adapters and subjected to size selection using agarose gel electrophoresis. Suitable fragments were amplified by PCR, and the cDNA library was sequenced using an Illumina HiSeq 2000 sequencer at the Beijing Genomics Institute (BGI; Shenzhen, China).The image data output from the sequencer was transformed into sequence data called raw reads. After filtering low-quality reads and reads containing more than 5% unknown nucleotides, the sequencing adaptors were removed from the raw reads. Subsequently, the raw reads were assembled into contigs and unigenes by de novo assembly, which was performed with the Trinity program