GSM2552921: Nab2_0_wt; Saccharomyces cerevisiae; OTHER
Cells were lysed, followed by stringent, denaturing, two-step affinity purification of the tagged protein. Bound RNA was partially degraded with RNase, radiolabeled, and 5’ and 3’ linkers were added. Finally, covalent RNA-protein complexes were isolated by SDS-PAGE. Following Proteinase K digestion of the bound proteins, RNA fragments were amplified by RT-PCR. RNA libraries were prepared using the standard CRAC protocol (Tuck and Tollervey, 2013)
GEO Accession
GSM2552921