GSM3523246: RNA-seq_HSPC_A; Homo sapiens; RNA-Seq
Total RNA was extracted from 1-2x106 HSPC, E-Prog and E-Prec (3 biological replicates for each stage) using RNeasy Micro kit (QIAGEN). RNA quality was assessed using the Agilent High Sensitivity RNA ScreenTape System (Agilent Technologies): RNA Integrity Number (RIN) was > 8 for all the samples. Libraries were prepared starting with 1 µg of total RNA using TruSeq Stranded mRNA kit (Illumina) and 100 bp paired-end sequences were generated on the HiSeq 2500 instrument (Illumina).
GEO Accession
GSM3523246