RNA-seq of  Equus Caballus: adult female BAL cells
RNA sequencing  libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for  Illumina following manufacturers instructions (NEB, Ipswich, MA, USA).  Briefly, mRNAs were first enriched with Oligo(dT) beads. Enriched mRNAs were fragmented for 15  minutes at 94 C. First strand and second strand cDNAs were subsequently  synthesized. cDNA fragments were end repaired and adenylated at 3ends, and  universal adapters were ligated to cDNA fragments, followed by index addition  and library enrichment by limited-cycle PCR. The sequencing libraries were  validated on the Agilent TapeStation (Agilent Technologies, Palo Alto, CA,  USA), and quantified by using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad,  CA) as well as by quantitative PCR (KAPA Biosystems, Wilmington, MA, USA).