ITS_Seq of fungi: adult human gut
The internal transcribed spacer (ITS) region was amplified using fungal-specific primers  ITS1F and ITS4  and the HotStarTaq Plus Master Kit. Amplicons from different samples were pooled to equimolar concentrations and purified of short fragments using Agencourt Ampure beads. Paired-end 2 x 250 bp sequencing was performed on an Illumina MiSeq instrument at Mr DNA .