GSM1668527: Population STARR-seq Rep 7; Homo sapiens; OTHER
Total RNA was purified via RNA midi prep (Qiagen) including DNase I treatment steps.  70 ug of RNA total RNA was used as input for poly-a selection and ribosomal RNA depletion using the dynabead OligoDT mRNA purificatin kit (invitrogen). RNA was treated with turbo DNase (invitrogen) then repurified using a a RNAeasy colum (Qiagen). cDNA was generated using SuperScript III reverse transcriptase using a reporter gene specific primer that bound the transcript generated from the plamsid. Libraries were constructed in two rounds. cDNA generated from spliced mRNA was amplified using a splice specific PCR primer. Then sequencing libraries were amplified from the reporter cDNA as per methods section.
GEO Accession
GSM1668527