GSM4160682: islet_RNA-Seq_rep2; Homo sapiens; RNA-Seq
RNA was extracted using Qiagen Rneasy kit Libraries were prepared using the protocol from Frazer lab. Briefly, RNA were first poly A selected and then subject to RNA fragmentation. Then fragmented RNA were proceeded with first stand synthesis, second strand synthesis, end repair and A-tailing. Resulting product was ligated with adaptor and selected by size, and next were enriched using PCR. At last DNA purification was performed and samples sent out for sequencing.
GEO Accession
GSM4160682