GSM3373928: GPY231 Rep3 [miRNA]; Homo sapiens; miRNA-Seq
TRIZOL reagent (Invitrogen) was used to extract total RNA from cells following the manufacturer's protocols. For small RNA librarires, 18-30 nt RNAs were isolated from total RNA of dissected ovaries using 15% polyacrylamide gels and libraries were constructed using the Illumina TruSeq smallRNA Library Kit. And the libraries were sequenced with an Illumina HiSeq2500 in 50-bp single-end fashion.
GEO Accession
GSM3373928