GSM2730584: Haploid_PostZGA_H2AFV_ChIP-Seq_Rep2; Danio rerio; ChIP-Seq
Whole embryos were cross-linked with 2.2% formaldehyde, then nuclei were extracted as described in Potok et al. Cell 2013. Nuclei preps were then combined and sonicated using a Branson Sonicator. Antibodies were then added to each lysate to bind overnight. The following day protein A/G dynabeads were then used to precipitate antibody bound chromatin (bead binding was for 4 hours at 4C). Bead bound chromatin was then washed and eluted using standard ChIP techniques. NEBNext ChIP-Seq Library Prep Reagents
GEO Accession
GSM2730584