GSM2807328: Cassanese_100DAF; Olea europaea; miRNA-Seq
Total RNA was extracted from the epi-mesocarp tissues of drupes collected together, using the RNeasy Plant Mini kit (Qiagen) according to the manufacturer’s instructions. Each RNA sample was subjected to DNase digestion (Dnase I, Roche) to remove any DNA contamination and pooled equally. Library preparation and sequencing was carried out by Technology Services of the Institute of Applied Genomics (IGA, Udine, Italy). Workflow starts with isolated total RNA. Upon the quality control of the starting material libraries are generated and size is selected in a range that maximizes the number of relevant reads. Sequencing is performed on HiSeq2500 in a 50 bp single-read mode.
GEO Accession
GSM2807328