GSM2113349: Wild-type Ebola DMSO; Homo sapiens; RNA-Seq
1 ug of RNA was treated using the Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat, Illumina), and purified post-depletion with 1.6x ratio of AMPureXP beads. Directional RNA libraries were prepared using NEBNext Ultra Directional RNA library prep kit for Illumina (New England BioLabs Inc.), according to manufacturer’s instruction. Fragment size distribution and concentration of the PCR amplified libraries were assessed using the Qubit and the Agilent Bioanalyzer. Finally, samples were sequenced on the HiSeq2500 platform in a 100bp single-end read format.
GEO Accession
GSM2113349