RNA-Seq of Caulobacter crescentus NA1000; wildtype; mid-log phase; biological replicate A
Caulobacter crescentus strain NA1000 was grown aerobically at 30 C in minimal medium (M2). Total RNA was extracted from mid-log (OD600 0.7) cultures (1 ml) using Qiagen RNeasy Plus Mini Kit (Qiagen) in accordance with the manufacturer's instructions. RNA samples were then submitted to the removal of ribosomal RNA using Ribo-Zero Magnetic Kit (Illumina). The quality of the isolated RNA and the percentage of rRNA present were checked by running RNA 6000 Pico Kit using Agilent 2100 Bioanalyzer (Agilent). Complementary DNA was generated according to instructions of TruSeq RNA Sample Prep Kit v2 (Illumina). The sizes of cDNA libraries were verified using DNA 1000 Kit (Agilent) and were further normalized to 4 nM using Kapa Biosystems kit (Kapa Biosystems) for library quantitation prior to cluster generation. The samples were sequenced using MiSeq Reagent Kit v2 (Illumina) in accordance with the manufacturer's instructions. This is the biological replicate A. One may use the following sequences in order to trim the adapters from FASTQ files: Read 1: AGATCGGAAGAGCACACGTCTGAACTCCAGTCA; Read 2: AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT