GSM2460787: fib; Homo sapiens; RNA-Seq
The sorted TRA1-60+ cells during reprogramming were washed several times in 0.5% BSA-PBS (Sigma) solution and subsequently picked and transferred into lysate buffer by a mouth pipette with 50 cell for each tube. The amplified cDNA was fragmented using Covaris sonicator (Covaris S220). To generate the sequence libraries, KAPA HyperPlus Library Preparation kit (KAPA KK8510) was used following the manufacturer’s instructions.
GEO Accession
GSM2460787