GSM3595457: ONT_683_SupCol; Homo sapiens; RNA-Seq
RNA was extracted using the QIAGEN miRNeasy kit. RNA CaptureSeq was performed as per the protocol from Mercer, T.R. et al Nature Protocols 9, 989-1009 (2014), with several modifications for the ONT platform. 1 µg of each of 4 human brain samples was spiked with RNA sequins (Mix A) at 2% fractional abundance. Following reverse transcription, PCA adapters from the ONT ligation sequencing kit 1D (SQK-LSK108) were ligated onto the end-prepped samples. The captured samples were barcoded using ONT 1D native barcoding genomic DNA kit (EXP-NBD103), and library prep was performed using the 1D genomic DNA by ligation kit and protocol for the PromethION sequencer (SQK-LSK109).
GEO Accession
GSM3595457