GSM3732546: T12: IFNgKO_SEB_3; Mus musculus; RNA-Seq
Small intestinal tissues were collected and stored in RNAlater untill processing. RNA from tissue samples was extracted using Trizol and further purified using Qiagen columns following manufacturers' instructions. Total RNA with RIN ≥ 8.0, was converted into a strand-specific library using Illumina's TruSeq Stranded mRNA HT Sample Prep Kit (Illumina, RS-122-2103), for subsequent cluster generation and sequencing on Illumina's NextSeq. The library was enriched by 13 cycles of PCR, validated using Agilent TapeStation and quantitated by qPCR. Individually indexed cDNA libraries were pooled and sequenced on NextSeq
GEO Accession
GSM3732546