GSM4662117: KM3F: cKO Female Mesometrial Decidua; Mus musculus; RNA-Seq
Freshly collected gestation day 8.5 mesometrial and anti-mesometrial decudua from littermate  control and Foxa2 conditional knockout uterus were snap frozen in liquid nitrogen. Total RNA was isolated from frozen speciment using a standard TRIzol-based protocol (Catalog 15596026, Thermo Fisher). To eliminate genomic DNA contamination, extracted RNA was treated with DNase I and purified using a RNeasy MinElute Cleanup Kit (Qiagen). The quality and concentration of RNA were determined using a Fragment Analyzer (Advanced Analytical Technologies). Libraries were prepared by Novogene using an Illumina TruSeq mRNA kit (Illumina Inc.) and sequenced (paired-end 150; 30 million read pairs) using an Illumina NovaSeq 6000.
GEO Accession
GSM4662117