GSM4161115: Saccharomyces cerevisiae_rep2_input; Saccharomyces cerevisiae; OTHER
Total RNA was isolated from cell lines with TRIzol reagent. For poly(A)+ RNA purification, mRNA was extracted by two successive rounds of poly(A)+ purification using Oligo d(T)25 Magnetic Beads (NEB). The untreated and pyridine borane treated samples were performed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® following its manufacturer's instruction in parallel for Illumina sequencing.
GEO Accession
GSM4161115