Comparative genomics of Pseudomonas aeruginosa rugose small colony variants
Sequencing libraries were prepared by transposase-catalyzed fragmentation and adaptor ligation using the Nextera DNA Sample Preparation Kit (Epicentre). Transposition reactions contained 50 ng gDNA in a volume of 5 ?l, with 0.1 ?l transpose enzyme and 1 ?l 5  Buffer HMW. Real-time PCR was used to amplify each library with a unique 9-mer barcoded adaptor, using the FailSafe PCR kit with Buffer K (Epicentre) and SYBR Green I at 0.25  final concentration.