GSM2043200: P493_Myc; Homo sapiens; ChIP-Seq
Cells were treated with 1% formaldehyde for 10 minutes at room temperature to crosslink protein-DNA complexes, and reactions were then quenched using 125 mM glycine for 5 minutes. The cells were then lysed with sodium dodecyl sulfate (SDS) lysis buffer and sonicated for 10 minutes using the Diagenode Bioruptor. The fragmented chromatin was pre-cleared with bovine serum albumin (BSA), protein A Sepharose beads (Millipore) at 4 ˚C for 2 hours before immunoprecipitation with c-MYC or rabbit control IgG antibodies overnight at 4 ˚C. Sepharose beads were washed as described previously and eluted with SDS elution buffer before subjecting to reverse cross-linking at 65 ˚C overnight. Finally samples were purified using the QIAquick PCR Purification Kit (Qiagen) according to the manufacturer’s protocol. Single read - Illumina
GEO Accession
GSM2043200