GSM3466711: dcl5_4_2mm_1_RNAseq; Zea mays; RNA-Seq
Total RNA for sRNA-seq and RNA-seq libraries was isolated using the PureLink Plant RNA Reagent (ThermoFisher) following the manufacturer's instructions. Total RNA quality was assessed by denaturing agarose gel electrophoresis and quantified by the Qubit RNA BR Assay Kit (ThermoFisher). For small RNA library preparation, 20 to 30 nt RNAs were excised from a 15% polyacrylamide/urea gel, and ~25 ng of sRNA used for library construction with the TruSeq Small RNA Prep Kit (Illumina) following the manufacturer's instructions. For RNA-seq, 2 μg of total RNA was treated with DNase I (New England BioLabs) and then cleaned with RNA Clean and Concentrator-5 (Zymo Research). The TruSeq Stranded Total RNA with RiboZero-Plant Kit (Illumina) was used for library construction with 500 ng of treated RNA, following the manufacturer's instructions.
GEO Accession
GSM3466711