GSM3673963: 44_RNa16R_CD4_Grp1_D3; Macaca mulatta; RNA-Seq
RNA was purified using QIAGEN RNeasy Micro columns, and RNA quality assessed using Agilent Bioanalyzer. 1 ng of total RNA was used as input for cDNA amplification using 5' template-switch PCR with the Clontech SMART-Seq v4 Ultra Low Input RNA kit, according to manufacturer's instructions. Amplified cDNA was fragmented and appended with dual indexed bar codes using Illumina NexteraXT DNA Library Prep kits. Libraries were validated by capillary electrophoresis on an Agilent 4200 TapeStation, pooled, and sequenced on an Illumina HiSeq 3000 using (101 bp SR) at an average read depth of 23 million reads/sample.
GEO Accession
GSM3673963