GSM2113429: LNT-229_20-4-15; Homo sapiens; RNA-Seq
Total RNA was prepared from between 1 to 2*106 GBM cells, using the Total RNA Purification Plus kit (Norgen biotec) as per the kit instructions. The isolated total RNA was quantified using Qubit 2.0 machine (Invitrogen) before the sequencing library preparation. The mRNA population in each sample was enriched by removing ribosomal RNA (rRNA) using the Epicenter ScriptSeq Complete kit (human, Illumina) as per the kit instructions. The experiment was performed by Illumina HiSeq 2500 in 50 base pair single read mode in the Technion Genome Center. The RNA-seq reads were mapped to the GRCh38 genome using TopHat version 2.0.13. Only uniquely mapped reads were counted in the analysis, using the HTSeq-count package version 0.6.1 with ‘union’ mode. The counts normalization and the differential expression analysis were done using the DESeq2 package version 1.8.1.
GEO Accession
GSM2113429