MBD-Seq of human: primary endometrial cancer tissue
DNA derived from a primary endometrial cancer tissue sample was subjected to fragmentation using a Covaris S2 Adaptive Acoustic instrument.  The fragments were enriched by MBD2 protein (MethylMiner Methylated DNA Enrichment Kit; Invitrogen) as described by the manufacturer's protocol. Library generation was performed according to the manufacturer's standard protocol.