RNA-Seq of Vitis vinifera: cv  Trajadura aerial parts
Plant material (approx. 30 g) from three to five genetically identical biological replicates was ground to fine powder in liquid nitrogen and used for total RNA extraction employing GPS buffer (0.2 M glycine, 0.1 M Na2HPO4, 0.6 M NaCl, pH 9.5), supplemented with 1 ml of 10% SDS, 0.1 ml of mercaptoethanol, 10 ml of water saturated phenol containing 0.1% 8-hydroxyquinoline and 10 ml of chloroform-pentanol (25:1). Samples were centrifuged at 8,000 g for 20 min at 4o C and the aqueous phase containing cellular nucleic acids was submitted to Whatman CF- 11 cellulose or BioRad Cellex N-I and eluted with STE buffer (0.1 M NaCI, 0.05 M Tris, 0.001 M Na. EDTA, pH 7.0)