Microbiota of Human gastric tissue: Sample GC32
The concentration of amplicons of approximately 280 bp was determined with Qubit dsDNA HS Assay Kit and the respective size distribution with Qiaxcel DNA screening. Equal concentrations were used for library preparation to incorporate adaptor and barcode sequences. Sequencing was performed in the Ion PGM Torrent platform using Ion 316v2 Chips as follow: 50ng of amplified DNA was used to ligate the Ion Torrent A  and P1 adaptors; the DNA molecules entered in a process of clonal amplification through an emulsion PCR using the OneTouch2 instrument. During emPCR single DNA fragments become bound via the specific adaptor to single ISPs. This process leads to coating of each ISP with millions of copies of a single DNA fragment. To ensure only coated ISPs proceed for sequencing, an enrichment step was performed using the OneTouch ES instrument.