GSM3733678: SAM24336973 Bone Marrow Parental; Mus musculus; RNA-Seq
Total RNA was extracted from control Luc gRNA iMACs and Irf2 gRNA2 KO iMAC lines (n=3) using a RNeasy Kit (QIAGEN) with on-column DNase digestion. QC of total RNA was done to determine sample quantity and quality. The concentration of RNA was determined using NanoDrop 8000 (Thermo Scientific) and the integrity of RNA was determined by Fragment Analyzer (Advanced Analytical Technologies). 100 ng of total RNA was used as an input material for library preparation using TruSeq Stranded Total RNA Library Prep Kit (Illumina).
GEO Accession
GSM3733678