RNA-Seq of F. pentaphylla: cold stress
Oligo (dT) magnetic beads were used to enrich mRNA, and then the mRNA was broken into short fragments.Using mRNA as a template, a single-strand cDNA was synthesized with random hexamers, and then buffers, dNTPs, and DNA polymerase I were added to synthesize the second-strand cDNA.The double-stranded cDNA was purified, end-repaired, A-tailed and connected to a sequencing adapter, and finally PCR enriched to obtain the final cDNA library.