GSM3373923: WT231  Rep1 [RNA]; Homo sapiens; RNA-Seq
TRIZOL reagent (Invitrogen) was used to extract total RNA from cells following the manufacturer's protocols. First, Total RNA was treated with the Epicentre Ribo-Zero kit (cat.#MRZH11124) to remove the all the rRNAs. The remaining RNAs were processed using the TruSeq RNA Sample Prep Kit according to the Illumina protocol. After cDNA synthesis, 6.5 pmol of DNA from each sample were clustered and sequenced on the Illumina HiSeq X-Ten (Illumina) in 150 bp paired-end reads.
GEO Accession
GSM3373923