GSM3466501: THP-1-shPRMT1-rep1; Homo sapiens; RNA-Seq
Total RNA was extracted using Trizol Reagent (Thermo Fisher Scientific) following the manufacture's instructions. RNA quality was assessed by spectrophotometry(NanoDrop, ND1000) and gel electrophoresis. To be considered as high-quality, the RNA had to have the result of A260/A280≧1.8, A260/A230≧1.5, no gDNA contamination. RNA integrity was measured by Agilent RNA 6000 Nano Assay (RIN≧6). RNA sequencing libraries were prepared with Kapa RNA HyperPrep kit with polyA kit (Kapa Biosystems, Cat KR1352) according to the manufacturer's protocol. Sequencing run was performed in the single read mode of 51cycle of read1 and 7 cycles of index read using Illumina HiSeq 2500 with HiSeq SBS V4 Kits. Real-time analysis (RTA) 2.2.38 software was used to process the image analysis.
GEO Accession
GSM3466501