Anchor-Seq (CDS-tag junction) of FACS binning (2nd FACS) of FACS enriched (1st FACS) lib #1.1
Random fragmentation of gDNA by sonication followed by end-repair and generic adapter ligation . Enrichment of junction fragments using tag- and adapter-specific primers. Libraries were size-selected for ~500 bp. Amplicons were than attached to motor-protein-loaded adapters as indicated by ONT. Informative read segements were extracted and demultiplexed using a custom script.