GSM4661597: mexR*-Exponential; Pseudomonas aeruginosa; RNA-Seq
Total RNA was obtained as describedm in Schuster et al. (2003) using the RNeasy mini kit (QIAGEN). RNA samples were sequenced at the "Centro Nacional de Análisis Genómico" (CNAG), Barcelona (Spain). Ribosomal RNA was removed using "RiboZero rRNA Removal kit for Bacteria" (CNAG). To generate the libraries, 2 μg of RNA were treated with "TruSeq RNA sample preparation kit" (Illumina) combined with a specific strand labelling using dUTPs (Sultan, M., et al., 2012). The sequencing in 2 x 75 pair-end format with Illumina technology was performed.
GEO Accession
GSM4661597