16S rRNA amplicon: low biomass hot springs
Bacterial and archaeal 16S rRNA gene sequences were targeted using the primers 515f and 806rB (Caporaso et al., 2012; Apprill et al., 2015). For sequencing, total DNA was submitted to the University of Minnesota Genomics Center and amplicons were sequenced using MiSeq Illumina 2300 bp chemistry. Each sample was sequenced once.