GSM2730426: CON_60; Homo sapiens; RNA-Seq
Citrated blood was fractionated by density gradient centrifugation using Ficoll-Paque Plus (GE Healthcare).  Neutrophils were then further purified by dextran sedimentation of the red blood cell layer, before lysing residual red blood cells with 0.2% sodium chloride.  Neutrophil preparations were at least 95% pure as confirmed by both flow cytometry and nuclear morphology. Purified neutrophils were resuspended in TriPure Isolation Reagent (Roche) and stored at -80 degrees.  RNA was later prepared by the Direct-zol RNA MiniPrep kit (Zymo Research) according to manufacturer’s instructions.  RNA Integrity Number (RIN) was >7 for all included samples. Sequencing libraries were created using the TruSeq Stranded mRNA library kit (Illumina) and 100bp single-end mRNA reads were generated on an Immunina HiSeq 2500 instrument.
GEO Accession
GSM2730426