GSM4160325: KO 5wk-B114; Mus musculus; RNA-Seq
Whole islets were isolated and total RNA purified using the RNAqueous Kit (Ambion).  Samples were further DNAse treated with the TURBO DNA-free kit (Ambion) as per the manufacturer's instructions.  RNA integrity and concentration was determined by Bioanalyszer and Qubit. PolyA based cDNA libraries were prepared from 5 week islet RNA samples using the services of Australian Genome Research Facility (AGRF).
GEO Accession
GSM4160325