GSM3070236: MDA-MB-231p27CK-DD_rep2_RNA-seq; Homo sapiens; RNA-Seq
Total RNA was extracted from cell lines with a TRIzol reagent kit (Invitorogen), according to the manufacturer's instructions. The quality of total RNA isolated from the cell lines was measured using Bioanalyzer RNA Nano 6000 (Agilent Technologies, Santa Clara, CA, USA). Library preparation was performed by TruSeq Standed Total RNA Library Prep (Illumina, San Diego, USA), and the quality was confirmed using KAPA qPCR Library Quantification (Kapa Biosystems, Wilmington, MA, USA). Paired end sequencing was performed on Illumina 150 cycles 400M reads.
GEO Accession
GSM3070236