SCT-10-10-60-2
Lactobacillus rhamnosus strains with higher(namely SCT-10-10-60) and lower(namely JCM1553) L-Lactate producing ablities were selected. Each had 3 replicates. Total mRNA was prepared using Ribo-zero Kit. Amplication template prepared by Fragmentatation of mRNA with proper buffer. First Strand synthesed with random haxamers as primers . Second strand synthesed with DNA polymerase and RNAase H and purified with AMPure XP beads. The product was then end-fixed,A-tail and adaptor added,size screened. A PCR using that product as template was carried out and the PCR product was purified by AMPure XP to make the prepared library.