GSM2044447: Diabetic_EP300_siRNA_R2; Homo sapiens; RNA-Seq
Total RNA was extracted in TRIzol (Invitrogen) and purified using the Direct-zolTM RNA MiniPrep kit (cat. no: R2052, Zymo Research) according to the manufacturers instructions. RNA was quantified on the MultiNA bioanalyzer (Shimadzu). NEBNext® Poly(A) mRNA Magnetic Isolation Module was used to enrich mRNA from 1 μg of total RNA. The NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® was used to generate barcodes. Libraries were quantified on the MultiNA bioanalyzer (Shimadzu) and pooled to equimolar ratios for sequencing. Cluster generation was performed at a concentration of 12 pM (TruSeq SR Cluster Kit v4-cBot-HS) and the flow cell was run on Illumina HiSeq2500 generating 50 nt reads.
GEO Accession
GSM2044447