GSM3523601: E12196 SCL-exo; Homo sapiens; OTHER
MM cells were purified from bone marrow samples using anti-CD138 MACS microbeads (Miltenyi Biotech). Genomic DNA was extracted using a DNeasy Blood and Tissue kit (Qiagen, France). Ten micrograms of genomic DNA from MM patients or cell lines were subjected to the SCL-exo protocol (Sérandour et al., Genome Biol, 2016). Libraries were obtained using the TruSeq ChIP Sample Prep Kit from Illumina and quantified using the KAPA library quantification kit (KAPA Biosystems). Libraries were sequenced (50bp, single end) with HiSeq 1500 (Illumina).
GEO Accession
GSM3523601