R. microplus genomic Cot 696 DNA
The Cot filtration was performed as previously described with some modifications (Peterson et al., 2002a), to enrich for single/low-copy and moderately repetitive DNAs. Starting DNA concentrations was 200 micrograms of sheared genomic DNA. Time for renaturation was 1 hr, 48 min, 6 sec for sample Cot 696.