GSM2112950: Selplg-/- 2; Mus musculus; RNA-Seq
Sorted WT or PSGL-1 KO CD8+ T cells were washed, pelleted, and frozen in RLT +BME Buffer (Quiagen RNeasy Micro Kit Cat. no. 74004). Total RNA extraction was completed using Quiagen Kit following manufactuer's instructions. 1ug of Total subjected to Ribo depletion to remove rRNA and then Multiplexed library was constructed using Illumina's Truseq Stranded Total RNA LT Kit Cat # RS-122-2201
GEO Accession
GSM2112950