GSM2463152: BS_seq_MALE_MEF_2; Mus musculus; Bisulfite-Seq
DNA samples were bisulfite converted using EZ-DNA Methylation-Direct reagent (Zymo Research). Strand-specific libraries were made using the PBAT protocol described in (Milagre et al. 2017). Briefly, a random elongation reaction was performed with primers containing truncated versions of the illumina sequencing primers, and a PCR amplification was performed using KAPA Uracil+ ReadyMix (KAPA Biosystems) and illumina primer sequences. Pooling was conducted using sanger iTags. Genomic DNA and RNA were isolated from the same samples using the Allprep DNA/RNA kit (Qiagen).
GEO Accession
GSM2463152