Reduced representation bisulfite sequencing of Homo Sapiens: HUES8 human embryonic stem cells with inactivating mutations in TET1, TET2, TET3 and DNMT3B
Genomic DNA was isolated from HUES8 WT and HUES8 TKO hESCs. After end-repair and TruSeq adapter (Illumina) ligation the DNA-libraries were denatured followed by treatment with bisulfite.