TnSeq of MAC109: in vitro 7H11 agar w/o Malachite Green (0.5% glycerol, 10% OADC, 0.05% Tween-80)
TraDIS-style library prep. Sheared gDNA collected from Tn mutant pool. End repaired, A-tailed, ligated adapter. PCR amplified from adapter and transposon. Added Illumina adapters via hemi-nested PCR.