HMP sequencing benchmarking: Mock DNA communtiy samples
Each amplicon library was constructed by amplifying the16S rRNA gene using either the 968f/1492r or the 27f/534r primer pair. Primers contained DNA barcode sequences such as those described by Hamady et al. 2008, and the recommended 454 adapter sequences for using genomic sequencing kit. Amplification conditions were adapted from McKenna et al 2007.
library_strategy
targeted-locus
gene
16S rRNA V1-V3 region
gene
16S rRNA V6-V9 region