Genome sequence of Nitrosotenuis aquariensis
Genomic DNA for metagenomic sequencing was extracted from Ca. N. aquariensis enrichment culture using the Powersoil DNA Isolation Kit (MO BIO Laboratories) according to the manufacturer’s instructions. Enrichment culture samples (6 x 50 ml) were pelleted at 7197 x g for 30 minutes, supernatant was removed and discarded, and pellets were suspended in bead lysis solution for DNA extraction. Sequencing was performed at Los Alamos National Laboratories (LANL). A short-insert, paired-end library was constructed using NEBNext Ultra DNA library kit and sequenced with a single index using the Illumina platform (31) on a MiSeq Sequencing System (Illumina, San Diego, CA).