GSM2045583: OX replicate 2; Mus musculus; RNA-Seq
FACS-isolated MuSCs were snap-frozen. RNA was extracted using the Nucleospin RNA XS kit (Machery-Nagel) 10 ng RNA was reverse-transcribed with the SMARTer Ultra Low Input RNA for Illumina Sequencing – HV kit (Clontech), which uses oligo(dT) priming. cDNA was amplified by LD-PCR using the Advantage 2 PCR kit (Clontech) and then sheared with a Covaris S2. End repair, multiplexed adaptor ligation, and library amplification (13-15 cycles) were performed using the Ovation Ultralow DR Multiplex 1-8 kit (NuGEN). Libraries were sequenced (101 bp, paired-end) at the Stanford Functional Genomics Facility with a HiSeq 2000 (Illumina) to a depth of ~40 million reads.
GEO Accession
GSM2045583