Mus musculus (animal 29) H3K79me2 pattern of cortical progenitor cells at E14.5 NMRI
The NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, E7370S) was used to convert 10 ng immunoprecipitated DNA (IP-DNA) and Input-DNA into indexed libraries for next-generation multiplex sequencing on the Illumina platform. Sequencing adaptors (1.5 µM) were ligated to end repaired and dA-tailed DNA fragments. A total of 10 PCR cycles was used to enrich adapter-ligated DNA fragments. Both sequencing adaptors and PCR primers were provided from NEB  (NEBNext Multiplex Oligos for Illumina (Index Primers Set 1 E7335, or Index Primer Set 2 E7500S). Final libraries were qualified using the Qubit fluorometer and the Agilent Bioanalyzer in combintion with the High Sensitivity DNA kit to meassure DNA concentration and visualize size distribution of respective libraries. Samples were sequenced paired-end with a read length of 50 bp to the desired depth using Illumina's HiSeq2500 and the respective sequencing protocols.