Whole exome sequencing of mouse B cell leukemia: normal tail tissue
Library preparation was performed with 1ug genomic DNA, integrity was determined using Agilent Tapestation. Genomic DNA was sonicated to an average size of 175bp. The fragments were blunt ended, had addition of A base to 3 end, and had Illuminas sequencing adapters ligated to the ends. The ligated fragments underwent amplification for 8 cycles. Fragments were hybridized to biotinylated RNA oligos (Agilent SureSelect Mouse Exome) specific to regions of interest, and selected from remaining fragments using streptavidin beads. Enriched fragments were amplified for 11 cycles with primers that incorporate a unique indexing sequence tag. The resulting libraries were sequenced using the Illumina HiSeq-3000 as paired end reads extending 150 bases from both ends of the fragments.