GSM2463084: d18_ev_R4; Caenorhabditis elegans; RNA-Seq
Samples were flash frozen in Trizol regagent and RNA was extracted using standard protocols. Libaries were prepared using Illumina's TruSeq stranded Total RNA LT Sample Prep Kit following Illumina’s standard protocol (Part # 15031048 Rev. E). The ribozero steps of the protocol for rRNA depletion were carried out manually, while the following steps of library preparation were performed using the Bravo Automated Liquid Handling Platform (Agilent). Libraries were prepared from a starting amount of 500ng of total RNA and amplified in 11PCR cycles.
GEO Accession
GSM2463084