GSM3526453: ChIP-seq+tet-DMS; Homo sapiens; ChIP-Seq
The ChIP was performed with an EZ-ChIP kit (Millipuro) following the manufacture's instruction. In this experiment, the DNA fragments were sonicated between 100bp to 500bp. For H3K4me3 ChIP, 2 X 107 cells were used. As to RNA-seq, total RNA was extracted using TRIZOL Reagent (Life technologies) following the manufacturer's instructions and checked for a RIN number to inspect RNA integrity by an Agilent Bioanalyzer 2100.Qualified total RNA was further purified by RNeasy micro kit and RNase-Free DNase Set. Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample ChIP-sequencing libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303). RNA-sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.
GEO Accession
GSM3526453