GSM3466736: A549; Homo sapiens; miRNA-Seq
Total RNA from the cell culture was isolated by TRIzol reagent (Invitrogen, CA, USA) and chloroform, according to the manufacturer's protocol. Total RNA concentration and purity were measured by a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, MA, USA). RNA integrity was measured by 2100 Bioanalyzer (Agilent, CA, USA) and agarose electrophoresis. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM3466736