Illumina HiSeq 4000 sequencing; Single cell RNA-seq of mouse skin cells
Single cell RNA-seq of mouse skin cells
Skin samples from two 8-week old female C57BL/6 mice were processed, first by mechanical processing, followed by 2 h incubation with 0.5% collagenase B (Roche; 11088815001). Skin samples from two 8-week old female C57BL/6 mice were processed, first by mechanical processing, followed by 2 h incubation with 0.5% collagenase B (Roche; 11088815001). Cells were then counted and loaded on the 10x Chromium machine. Libraries were prepared following the Chromium Single Cell 3′ v2 Reagent Kit Manual54.
Experimental Factor: individual
3421STDY7639029