GSM2883199: E18_5_wholeThy_1; Mus musculus; RNA-Seq
Thymic lobes were mechanically isolated, dissociated in trypsin, depleted of RBC's (only some samples), washed and stained with 7AAD cell viability dye, and 7AAD negative single cells were sorted on a BD FACSARIA. Libraries were prepared according to the Drop-seq lab protocol version 3.1 (Macosko and Goldman, December 2015, McCarroll Lab).  Final libraries were sequenced on an Illumina NextSeq 500 using high-output 75-cycle kits.
GEO Accession
GSM2883199