GSM3595710: wild_type_no_target_Cas9_2; Mycobacterium tuberculosis H37Rv; RNA-Seq
Bacteria were harvested during the logarythmic phase of growth. RNA was extracted with Trizol LS(Thermo Fisher Scientific, Waltham, USA) following standard laboratory procedures used for mycobacteria (ribolysing the cells with "lysing matrix B" in MP FastPrep homogenizer). KAPA Stranded RNA-Seq Library Preparation Kit (cat#KK8401 , from KAPA Biosystems), following ribodepletion of samples with Ribo-Zero(Bacteria)(from Illumina, San Diego, USA)
GEO Accession
GSM3595710