GSM3069670: Pvir#6_hep; Procambarus virginalis; Bisulfite-Seq
Samples of organs and tissues from adult crayfish for DNA and RNA preparation were taken under a dissection microscope, snap-frozen in liquid nitrogen and stored at -80°C until extraction of nucleic acids. Embryonized eggs and juveniles were frozen in liquid nitrogen as a whole. Genomic DNA was isolated using the Blood & Cell Culture DNA Kit (Qiagen, Hilden, Germany) The TruSeq PCR-Free Library Prep Kit (LT) was used for library preparation, and the Epitect Kit (Qiagen) for bisulfite conversion. Library amplification was performed using the Kapa HiFi HotStart Uracil+ ReadyMix (2X). WGBS on an Illumina HiSeq platform
GEO Accession
GSM3069670