GSM3823860: MADRK27M113018-scATACseq; Mus musculus; ATAC-seq
Brains were collected in PBS and tumors were microdissected, mechanically dissociated and further enzymatically digested to obtain a single cell suspension. Single cell suspensions were immediately processed for 10X single cell ATAC-seq. scATAC sequencing library was prepared on the 10X Genomics Chromium platform following the manufacturer's protocol (10X Genomics 1000110). The isolated nuclei suspension was diluted and then incubated with transposition mix for a targeted nuclei recovery of 10,000 cells. GEMs were then captured on the chromium Chip E (10X Genomics 1000082). Following GEM incubation, clean up was performed using Dynabeads MyOne Silane beads (10X Genomics 2000048) and SPRIselect reagent (Beckman Coulter B23318). Finally, the library was amplified for a total of 10 SI PCR cycles.