Small RNA seq of Aedes albopictus larvae rep3
The total RNA of each pool was extracted using TRIzol reagent (Invitrogen USA) according to the manufacturers instructions. RNA quality was measured by Agilent 2100 Bioanalyzer system (Agilent Technologies, Palo Alto, Ca). Follow the steps below to construct small RNA libraries: (1) polyacrylamide gel electrophoresis (PAGE) purification of the RNA bands and RNA molecules in a size range of 1635 nt enriched; (2) ligation of the 3 and 5 adapters to the RNA; (3) PCR amplification to generate a cDNA library for Illumina sequencing. Small RNA libraries were sequenced using SE50 by Illumina Nextseq 500 according to manufacturers instructions.