GSM3069614: P8 WT_T25 RNAseq; Mus musculus; RNA-Seq
RNA from retinas was extracted in using the Qiagen RNAeasy kit (Qiagen, cat no. 74104) To generate RNA sequencing libraries, RNA quality and quantity were determined with the Agilent Bioanalyzer 2100, accepting RNA integrity numbers (RIN) of > 7 and quantities of 100 nanograms or more per sample. Directional mRNA libraries were prepared using Illumina TruSeq mRNA Sample Preparation Kits per manufacturer's instructions. Polyadenylated mRNAs were captured from total RNA using oligo-dT selection. Samples were converted to cDNA by reverse transcription. Each sample was ligated to Illumina sequencing adapters containing unique barcode sequences. Barcoded samples were PCR amplified, resulting cDNA libraries were quantified using qPCR. Equimolar concentrations of each cDNA library were pooled and sequenced on the Illumina HiSeq2500.
GEO Accession
GSM3069614