GSM3593560: NASH-diet-fed mouse (PEG minipump, Eartag#741); Mus musculus; RNA-Seq
Total RNA of 12 liver samples were extracted with Trizol reagent and cleaned up with Qiagen clean-up kit (Cat# 74204). Libraries were prepared with Roche KAPA mRNA HyperPrep Kit (KK8581) following the manufacturer's protocol and being paired-end sequenced (2 x 76bp) on Illumina HiSeq 4000 platform. Quality control for the raw sequence reads were analyzed with FASTQC (https://www.bioinformatics.babraham.ac.uk/projects/fastqc/). Adaptors and low-quality reads were trimmed by Trimmomatic v0.35.
GEO Accession
GSM3593560