H3K27ac ChIP-seq of human Ileal NET sample CAR 11
Soluble chromatin (5 μg) was immunoprecipitated with 10 μg H3K27ac (Active Motif 39133) antibody. ChIP-seq libraries were constructed by end repair of precipitated DNA, base extension with Klenow fragment and ligation of Y-form-sequencing adapters, followed by 14 cycles of PCR. Between enzymatic steps, fragments of the desired size were enriched using AMPure XP beads.
alignment_software
BWA 0.7.10