GSM3223405: SUPT4H1_10_1: SUPT4H1 siRNAs, 10 nM, replicate 1; Homo sapiens; RNA-Seq
72 hours post transfection, total RNA was extracted with the RNEasy (Qiagen) kit from 25,000 cells per sample. Either ERCC mix1 or mix2 external standards (Invitrogen) were diluted 1:100 in water and 1 ul was added to each lysate. Libraries were prepared from 100 ng of total RNA using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer.
GEO Accession
GSM3223405