Full Length RNA-Seq of Iris japonica:shoot apical
The 12-, 23- and 36-kb cDNA fractions were generated with a BluePinppin size selection system. The three libraries were constructed with a Pacific Biosciences SMRTbell Template Prep Kit 1.0 following the vendors protocol. The libraries were subsequently sequenced on the PacBio RS II real-time (RT) sequencer platform using SMRT Cell 8 Pac v3 with a total of seven SMRT cells: the 12- kb library was sequencing using three SMRT cells; while 23- and 36-kb libraries used two SMRT cells, respecitively