RA_S_TCMID29
This study collected passively settled dust indoors and outdoors at a university housing complex. A 300bp region targeting the V1/V2 region of the bacterial 16S rRNA gene was amplified in triplicate and pooled using primers 8f/357r modified for 454 pyrosequencing. Amplicons were pooled and cleaned using Ampure Beads. Concentrations of the pooled amplified product were determined with Qubit and pooled to an equimolar concentration of 25ng for pryosequencing.