oocyte_Uhrf1_control2
Samples were collected into lysis tubes containing an RNase inhibitor. Samples were amplified by the Smart-Seq2 method.Anoligo-dT primer was used for reverse transcription of first-strand cDNA synthesis, followed by PCR amplification and then a mag bead purification step to clean up the cDNA. The amplified cDNA samplewas checked by a Qubit 3.0 Fluorometer (Thermo Fisher Scientific) and an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) to ensure the expected product length was ;12 kbp. The cDNA was then sheared randomly by ultrasonic waves for the Illumina library preparation protocol (Illumina, San Diego, CA, USA), involving DNA fragmentation, end repair, 39-end A-tailing, adapter liga- tion, PCR amplification, and library validation. After library preparation, the PerkinElmer LabChip GX Touch (PerkinElmer, Waltham, MA, USA) and Step OnePlus Real-Time PCR System (Thermo Fisher Scientific) were used for library quality in- spection. Suitable libraries were then loaded on aHiseq platform for PE150 sequencing (Illumina)