Transcriptomes of Solanum lycopersicum Leaf under cold stress at 0h, 1h and 12h
The plants grow at 25°C with 16 h light and 8h dark cycles for 8 weeks before harvested. All cold stress treatments were began at 4°C at 12 PM under light and continued for 0 (untreated control), 1 and 12 h. The total RNA from leave was extracted using TRIzol reagent (Invitrogen) and digested with RQ1 DNase (Promega) to remove genome DNA. RNA-seq libraries were prepared and applied to the Illumina Genome Analyzer IIx system for 80 nt single-end sequencing.