GSM2551142: Untreated F1 female brain Xist 2lox/+ (N10-2); Mus musculus; RNA-Seq
Total RNA was extracted using Trizol from tissue that was snap frozen in liquid nitrogen and grinded. Ribosomal RNA was removed using RiboMinus. Strand-specific libraries were generated by performing reverse transcription with Superscript III, followed by second-strand synthesis using the NEBNext second-strand synthesis module, and then libraries were generated using the NEBNext ChIP-seq Library Preparation Kit.
GEO Accession
GSM2551142