Illumina HiSeq 2000 paired end sequencing
Total RNA was isolated from germinating cysts of Phytophthora cinnamomi isolate CNVDB29 and sequenced on the Illumina platform with the aim of identifying pathogenicity genes.
A cDNA library of cysts and germinating cysts of P. cinnamomi isolate CNVDB29 was prepared through fragmentation of mRNA enriched RNA (oligodT-primers) and random hexamer primed cDNA synthesis.  The paired end cDNA library was sequenced on the HiSeq2000 Illumina platform.