GSM3593956: ESCs (SSEA1+/GFP+); Mus musculus musculus x M. m. castaneus; RNA-Seq
Briefly, sorted SSEA1+ reprogramming intermediates were immediately lysed in the RTL buffer (RNeasy Micro Kit (Qiagen, 74004)) and stored in -80°C until cells from all timepoints were collected. Next, all samples were processed together to extract RNA following manufacturer's protocol cDNA synthesis was done starting from 500 pg of input RNA, followed by library preparation from 80 pg of cDNA using Nextera XT kit (Illumina, FC-131-1096). Indexing was performed with the Nextera XT index Kit V2 (Illumina, FC-131-2003). The quality of input RNA, cDNA and individual libraries was assessed using Bioanalyzer (Agilent 2100 Bioanalyzer system).
GEO Accession
GSM3593956