environmental sediments: archaea pool 2 Kerlingarfjoll
Circular Consensus Sequencing was performed by MR DNA (Shallowater, TX, USA) on the PacBio Sequel using bacteria (27F-AGAGTTTGATCCTGGCTCAG and 519R-GTNTTACNGCGGCKGCTG) and archaea (21F- TCCGGTTGATCCYGCCGG and 505R- CCR TGC TTS GGR CCV GCC TGV CCG AA) specific primers. A depth of 5000 reads per sample was achieved for each 16S rRNA assay with an average postprocessing read length of 1400 bp. Sequence data was processed using the MR DNA analysis pipeline to remove barcodes, orientate sequences 5 to 3, and to remove sequences <150bp and sequences with ambiguous base calls.