GSM2807951: DEF2; Mus musculus; RNA-Seq
Skin samples were finely minced using scissors and digested for 45 min at 37 °C in RPMI medium containing DNase I, Collagenase I, Liberase and Dispace. For adult skin, epidermis was removed prior to cutting in an additional Dispase digestion step. Full length cDNA were generated from 10 ng of total RNA using the SMART-Seq v4 Ultra Low Input RNA kit for Sequencing (Clontech, Part number 634890) according to manufacturer's instructions with 10 cycles of PCR for cDNA amplification by Seq-Amp polymerase (Clontech). Six hundreds ng of pre-amplified cDNA were then used as input for Tn5 transposon tagmentation by the Nextera XT kit (Illumina, Part number FC-131-1096) followed by 12 cycles of library amplification. Following purification using Agencourt AMPure XP beads (Beckman Coulter, Part number A63882), the size and concentration of library DNA were assessed on an Agilent 2100 Bioanalyzer.
GEO Accession
GSM2807951