16S rRNA gene for control soil from microcosm expeiment at Day 21, replicate d
Fragments of 16S  rRNA gene were amplified with universal primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 909R (5'- CCCCGYCAATTCMTTTRAGT -3') with 12 nt unique barcode(Caporaso, et al. 2010: 335-6). PCR product was purified using the gel-purification kit. Purified product was quantified with Nanodrop and pooled with equal molar amount for each sample.