Targeted sequencing of a panel of 315 genes of human gastric cancer primary biopcy specimen
The assay employs a single DNA extraction method from routine FFPE biopsy or surgical resection specimens, 50-1000 ng of which will undergo whole-genome shotgun library construction and hybridization-based capture of all coding exons from 309 cancer-related genes, one promoter region, one non-coding (ncRNA), and select intronic regions from 34 commonly rearranged genes, 21 of which also include the coding exons. In total, the assay detects alterations in a total of 324 genes. Using the Illumina HiSeq 4000 platform, hybrid captureselected libraries are sequenced to high uniform depth (targeting >500X median coverage with >99% of exons at coverage >100X). The reads were mapped using bwa, version 0.5.9-r16