ABA treated rice aleurone: rep1 8905X3
Rice seeds were deembryonated and placed into imbibing solution overnight. The rice aleurone was isolated and placed into imbibing solution containing 20uM ABA for an additional 4 hours.  The aleurone was flash frozen with liquid nitrogen. The RNA was extracted via guanidium sulfate phenol chloroform extraction. See Watanabe et al. 2016 Plant Physiology.