Nano-OTS of human: HEK293 DNA
A Nano-OTS libary  was constructed using a modified version of the protocol described by  Gilpatrick et al. (2020), Nature Biotechnology. Instead of going in with  intact genomic DNA in the library construction, the DNA was sheared to 20 kb  using the Megaruptor 2 (Diagenode) and size-selected prior to library  construction. In addition, only one guide RNA per target was used. Guide RNAs  used in this experiment were designed to target the ATXN10, MMP14 and NEK1  genes. Sequencing was performed on the MinION system using one R9.4.1  flowcell.