GSM3523191: GT52 Week 2 Control [GT52_2_C]; Homo sapiens; RNA-Seq
Tissue biopsies were homogenised in 300μL of RLT buffer (Qiagen, GmbH, Helden, Germany) mixed with β-mercaptoethanol (Sigma-Aldrich, St Louis, MO) (10μl β-mercaptoethanol/ml RLT buffer) using 5-mm stainless steel beads and a Qiagen TissueLyser II (Qiagen GmbH) at 30 oscillations/second for 10 minutes. RNA was extracted using RNeasy Tissue Mini Kits (Qiagen GmbH). Eluted RNA quantity and purity was assessed using NanoDrop ND-100 v3.0.1 spectrophotometer (NanoDrop Technologies, Wilmington, DE), Agarose gel electrophoresis and Agilent 2100. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM3523191