[TEMPORARY] Normalized cDNA prepared from mixed-stage embryos of Gryllus bimaculatus
Total RNA was extracted from Gryllus bimaculatus embryos (mixed stages representing all of embryonic development).  Normalized cDNA was synthesized following the SMART cDNA library construction protocol (Clontech).  cDNA was normalized with the TrimmerDirect kit (Evrogen). Note that the SMART and TrimmerDirect adapters *must* be removed for assembly (e.g. with Newbler, using the -v flag, or using a program such as cross_match).  See <PMID> for details. >Evrogen_5'_adapter_undigested AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG >Evrogen_5'_PCR_Primer AAGCAGTGGTATCAACGCAGAGT >Evrogen5'_digested CGGCCGGG >Evrogen_CDS_3M_Adapter_undigested AAGCAGTGGTATCAACGCAGAGTGGCCGAGGCGGCCTTTTTTTTTTTTTTTTTTTT >Evrogen_CDS_3M_Adapter_minus_polyT AAGCAGTGGTATCAACGCAGAGTGGCCGAGGCGGCC >Evrogen_CDS_3M_Adapter_digested AGGCGGCCTTTTT