GSM3677528: E14.5 Adamts19-/- mouse heart r8; Mus musculus; RNA-Seq
Single cells were co-encapsulated with barcoded microbeads to produce single cell transcriptomes attached to microparticles (STAMPS) accodring to Drop-seq protocol. STAMPS were counted and PCR amplified for 13 cycles. Amplified cDNA was measured using Bioanalyzer and used for library preparation. A modified Nextera-XT protocol as described in Macosko et al. 2015 was used to construct libraries. Illumina i7 primer were used in combination with a custom i5 primer