gut 16S sequencing
           A region of the 16S rRNA gene was PCR amplified using derivatives of primers 27F/338R, which were modified to add a unique sequence tag to the amplicons from each sample in a pool as listed. Amplicons were quantified by picogreen and pooled to a final concentration of 100 ng per amplicon.         
library strategy
targeted locus
gene
16s rRNA V2 region