250 bp insert paired-end library
We extracted genomic DNA from the thorax of a single wild-caught H. misippus f. misippus female using phenol/chloroform protocol. We then constructed Illumina paired-end (PE) libraries using the KAPA Hyper Prep Kit (KR0961  v1.14) and 1 ug genomic DNA each, selecting for mean insert size ~250 bp.