RNA-seq of Dreissena rostriformis bugensis: adult
Digestive glands were dissected and stored in RNAlater (Qiagen) at 4C. Total RNA extraction was done using RNeasy MinElute kit (Qiagen) according to the manufacturers protocol with a slight modification. Briefly, tissus disruption was done first with a pestle and then with a beads beater with glass bead (200 m) for 30 sec at max speed. Genomic DNA was removed by DNase digestion on column and total RNA was eluted in ultra pure water. Total RNA was quantified with a nanodrop and RNA integrity was assessed on a bioanalyzeur (Agilent). Total RNA samples were stored at -80C.