GSM1239138: FROZ_TruSeq_BRB147; Homo sapiens; RNA-Seq
RNA was extracted from FFPE tissue using the Qiagen AllPrep FFPE kit including treatment with DNase. RNA was extracted from the fresh-frozen tumors using the Qiagen Rneasy including treatment with DNase. For Illumina libraries we used 200ng total RNA and followed the manufacturer's instructions for TruSeq RNA sample preparation V2 (catalogue number: RS-122-2001). For the RiboZeroGold/ScriptSeq V2 libraries (catalogue number: LIMC1204/SSV21124) we used 200-400ng total RNA and followed the manufacturers instructions, with the exception for FFPE libraries, we extended the cDNA synthesis 37C step to 90 minutes.
GEO Accession
GSM1239138