Trametes cingulata TCM6 genome sequencing using Roche 454 GS-FLX Titanium
1. DNA Fragmentation (Nebulization).     2. DNA Fragment Size Selection-double SPRI Method.     3. DNA Quality Assessment.     4. Fragment End Polishing.     5. Adaptor Ligation.     6. Small Fragment Removal.     7. Library Immobilization.     8. Fill-In Reaction.     9. Single-Stranded DNA Library Isolation.     10. DNA Library Quality Assessment and Quantitation.     All of the reagents used came within the GS-FLX Titanium Library Preparation Kit ordered from Roche.