Capture enriched library of etDNA of 10 invertebrate species  mock community number 6
DNA was sheared into approximately 600 nucleotide fragments by  ultrasound sonication. Library preparation was conducted using NEBNext  UltraTM II DNA Library Prep Kit for Illumina (New England BioLabs, USA)  following manufacturer's instructions. COI capture enrichment was conducted  using myBaits Custom kit following manufacturer's instructions (Arbor  Biosciences, USA). Baits were diluted 10 times and hybridization lasted 24 h  for both type of DNA. The final library amplification was performed with the  KAPA HiFi DNA Polymerase (Kapa Biosystems, USA) and reampP5 and reampP7  Illumina primers. Capture library concentrations were determined by qPCR with  a KAPA qPCR kit (KAPA Library Quant Kit, Kapa Biosystems, USA).