GSM4262971: ATAC-seq_Young sample_2; Mus musculus; ATAC-seq
Nuclei were isolated by cell resuspension in ice-cold lysis buffer (10mM Tris-HCl ph=7.4, 10mM NaCl, 3mM MgCl2, 0.1% NP-40, 1X protease inhibitor cocktail) and centrifugation at 4oC for 5min. The nuclear pellet was then resuspended in ice-cold 1X TD Ilumina buffer. Libraries were prepared using Nextera PCR primers and the NEBNext High-Fidelity PCR Master Mix
GEO Accession
GSM4262971