RNA-seq, mouse osteocytic cell line MLO-Y4 - static, replicate 3
Mouse osteocytic cell line MLO-Y4 was grow under fluid flow to compare with static one (no fluid flow) to study the effect of mechanical force that influe ence calciam chanels. Cells were havest and purified total RNA. Purified RNA was used as input for sequencing library preparation and indexing using the TruSeq stranded mRNA kit (Illumina, CA), following the manufacturers protocol. The libraries were then pooled and sequenced using a NextSeq sequencer with 75 cycles of sequencing reaction.