AccuNGS_in_vitro_RNA
CVB plasmid reversed transcribed using T7; random hexamers RT using SuperScriptIII; 1500bp amplicon, 40 PCR rounds with Superfi Platinum polymerase; PCR product extraction from agarose gel; Nextera XT library preparation kit with 12 PCR rounds using Superfi Platinum polymerase; library purification and size selection with AMPure XP beads; paired-end sequencing with Illumina MiSeq