Subject1, sample type=Stool, input DNA for library construction=50ng
PCR using primers targeting the V6 region of the 16S rRNA gene and incoporating barcodes on each end of the PCR fragment for barcoding. Amplicons were sequenced on a HiSeq2500 with 100bp paired end reads. The overlapping regions of the paired end reads were used to merge each paired end read into a single sequence and reads were subsequently quality filtered/demultiplexed.