GSM3521364: hst34 NET-seq rep2; Saccharomyces cerevisiae; OTHER
Cells were cryogenically pulverized for lysis and nascent RNA was isolated via an immunoprecipitation at 4°C of Rpb3-3xFLAG and purified using the miRNeasy kit (Qiagen, 217004). RNA Pol II IP, RNA purification, and library construction was carried out as described previously (Churchman and Weissman, 2011; Mayer et al., 2015). 3' end sequencing was performed on an Illumina NextSeq 500 with a read length of 75 bp.
GEO Accession
GSM3521364