GSM1240657: WT cells (set 2); Homo sapiens; RNA-Seq
Total RNA was extracted with TRIzol, and DNase I was treated. mRNA was enriched from total RNA by Oligo(dT)-Dynabead. The enriched mRNA was randomly fragmented in the Magnesium ion-containing buffer. The mRNA fragments ranging 70-90nt were fractionated, ligated to adaptors at both 5' and 3' ends, and reverse-transcribed for PCR amplification. The final PCR products were sequenced by HiSeq 2000.
GEO Accession
GSM1240657