16S amplicon sequencing of 1,2,4-TCB-enriched dechlorinating cultures: KB-1 TCB/methanol fed replicate 1
DNA was extracted and PCR-amplified using the universal primer set 926f (5?-AAACTYAAAKGAATTGACGG-3?) and 1392r (5?-ACGGGCGGTGTGTRC-3?). The PCR products were cleaned and checked via gel electrophoresis on a 2% agarose gel. Each PCR product was checked for quality and several samples were pooled for unidirectional sequencing using the Roche GS FLX Titanium technology.