RNA-Seq of Lampsilis siliquoidea: juvenile whole-body
Total RNA was extracted using Trizol reagent (ThermoFisher). RNA was purified using a phenol-chloroform extraction followed by a precipitation with ethanol and sodium acetate.  Extracted RNA was quantified using a Qubit 3.0 Fluorometer, and its quality was assessed using a NanoDrop One spectrophotometer and by visualizing the RNA on a 1% agarose gel.  RNA quality was assessed on a bioanalyzer prior to cDNA library construction using Illumina's TruSeq Stranded mRNAseq Sample Prep Kit (Illumina).  Sequencing was performed by the Roy J. Carver Biotechnology Center and pooled cDNA libraries were paired-end sequenced on a HiSeq 4000 across two runs and a total of four lanes.