RNA-Seq of Gallus gallusbroiler chicken treated by feed blank silkworm hemolymph
blank silkworm hemolymph treated chicken fat tissue(feed).Enrichment of messenger RNA (mRNA) by hybridization to Oligo-dT.Fragmentation was carried out using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer.And then do reverse transcript PCR.