GSM2045508: WT rep2; Arabidopsis thaliana; RNA-Seq
Plant tissues were flash frozen in liquid nitrogen, and Total RNA from apices were isolated using the mirVana™ miRNA Isolation Kit (lifetechnology). 10 μg of totalRNA were subject to DNase I treatment and mRNA was purified by Dynabeads® mRNA DIRECT™ Purification Kit(lifetechnology). The strand-specific RNA-seq libraries were prepared using NEBNext® UltraTM RNA Library Prep Kit for Illumina following the manufacturer's instructions and subjected to Illumina HiseqTM2500 for 2x100bp pair end sequencing. library source: transcriptomic; library selection: cDNA; instrument model: Illumina HiSeq 2500
GEO Accession
GSM2045508