GSM3466673: P60 brain pre-myelinating oligodendrocytes_RNASeq_n3 [plp2mn3]; Mus musculus; RNA-Seq
Total RNAs were obtained from FAC-sorted P60 oligodendrocyte progenitor cells and from P60 myelinating oligodendrocytes, isolated from mouse brains. We used AllPrep DNA/RNA Mini Kit (Qiagen), with on-column DNase treatment during the RNA isolation, in accordance with the manufacturer's protocol, to isolate DNA and RNA simultaneously from the same cell pellet. RNA purity was assessed by measuring the A260/A280 ratio using a NanoDrop, and RNA quality checked using an Agilent 2100 Bioanalyzer (Agilent Technologies). RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM3466673