16S rRNA of Oreochromis niloticus: gut Manzala
Collective DNA was isolated from the guts of Nile tilapia fish harboring salt-water lake Manzala in Northern Egypt. 16S rRNA gene was amplified using universal primers for hypervariable regions V2, V3, V4, V6-7, V8, and V9. Metagenomics library was constructed according to the Ion torrent manual. PCR amplicons were sequenced using the ion Torrent platform following the recommended protocol. Data sets were analyzed using (Ion Reporter Software of Thermo Fisher Scientific and phylogenetic analysis was done