GSM1232743: SigX_chip_sample1; Pseudomonas aeruginosa UCBPP-PA14; ChIP-Seq
Polyhistidine-tagged sigma-factors were isolated by using anti6Xhis-antibodies (abcam) and magnetic beads coupled with protein-G (Invitrogen) on a rotator. Immunoprecipitated DNA was linear amplified similar to the LinDA-ChIP-seq protocol published by Shankaranarayanan et al., Nature potocols, 2012. Library was constructed using the TruSeq DNA sample preparation kit according to the low-throughput protocol (Illumina).
GEO Accession
GSM1232743