Amplicon Sequencing of Mus musculus Male 0.4ppm
An in house standardized protocol was employed to extract the genomic DNA from the fecal pellet samples. Illumina standardized V3-V4 regions of 16S rRNA library protocol were employed for the preparation of the library. The library which was generated contained V3-V4 amplicons were then sequenced on IlluminaMiSeq using 300x2 PE chemistry.