Draft genome sequence of Lactobacillus fermentumLf2, an exopolysaccharide-producing strain isolated from Argentine cheese
Genome of L. fermentum Lf2 was determined by GenProbio srl (Parma, Italy) using the MiSeq Illumina (Illumina, USA). Genomic libraries were constructed employing the TruSeq DNA PCR-Free LT Kit (Illumina) and using 2.5g of genomic DNA, which was fragmented with a Bioruptor NGS ultrasonicator (Diagenode, USA) followed by size evaluation using Tape Station 2200 (Agilent Technologies). Library samples were loaded into a Flow Cell V3 600 cycles (Illumina) according to the technical support guide, and generated reads were depleted of adapter sequences, quality filtered and assembled through the MEGAnnotator pipeline (Lugli et al, 2016, FEMS Microbial Lett).