RNAseq of LCM microdissected ovules: sexual H. perforatum pool3
RA extraction from 250.000 m2 of tissue sections with the Stratagene Absolutely RNA Nanoprep Kit (Agilent). RNA was isolated according to the manufacturers instructions. cDNA synthesis and amplification were performed using the AMBION MessageAmp II aRNA Amplification Kit. Libraries. The TruSeq Stranded Total RNA Sample Prep Kit was used to prepare the libraries from 50 ng of aRNA. The generated barcoded library was sequenced on an Illumina NextSeq500 using 151nt reads in paired-end.