15 kb mate-pair library
We extracted genomic DNA from the thorax of a single wild-caught H. misippus f. misippus female using phenol/chloroform protocol.  We then constructed a mate pair (MP) librays with insert size of ~15 kb, selected using a BluePipping, using the Nextera Mate Pair Library Prep kit (FC-132-1001) and 4 ug genomic DNA each.