GSM2044396: miR23a_3; Mus musculus; RNA-Seq
150,000-800,000 live RosaYFP+CD4+ Dgcr8f/f Tbx21-/- Cd4-cre cells were sorted directly into Trizol LS (Life Technologies) and RNA isolated using a miRNeasy Micro Kit (QIAGEN) with on-column DNase digestion. RNA sequencing libraries were generated using 1ng of total RNA and the Ovation RNA-Seq System V2 and Ultralow Library Construction System sample prep kits (NuGEN). Libraries were multiplexed at a density of 4 per flow-cell lane and sequenced on the HiSeq 2500 to generate single end 50bp reads per the manufacturer’s instructions (Illumina). The following barcodes were used: 23a_1=AAGGGA, 23a_2=AAGACG, 23a_3=ACACGA, 23a_4=ACAAAC, 24_1=CCTTCA, 24_2=CCTCGG, 24_3=CACACA, 24_4=CACCTC, 27a_1=GGACCC, 27a_2=GGATGT, 27a_3=GTGTTA, 27a_4=GTGGCC, cont_1=TTCAGC, cont_2=TTCGCT, cont_3=TGTGAA, cont_4=TGTTGC.
GEO Accession
GSM2044396