GSM3223569: Control Garganega berries 3; Vitis vinifera; RNA-Seq
Infected/uninfected berry pericarps were ground into liquid nitrogen and total RNA was isolated from 200 mg of powders using the Spectrum™ Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO, USA). The RNA quantity, integrity and purity were controlled using a Nanodrop 2000 instrument (Thermo Scientific, Wilmington, DE, USA) and a Bioanalyzer Chip RNA 7500 serie II (Agilent). For each sample, unstranded library was prepared from 2.5 µg of total RNA using the Illumina TruSeq Library Prep Kit v2 (Illumina, San Diego, CA).
GEO Accession
GSM3223569