GSM2807427: P24_NT; Homo sapiens; RNA-Seq
Total RNA from 50 frozen patient tissues constituting the tumoral and adjacent NT and 2 cell lines was extracted using the mirVana kit (Thermo Fisher Scientific) according to the supplier's protocol. The RNA integrity number (RIN) was evaluated by Agilent 2100 Bioanalyzer. The total of 4 μg was used for generation of each poly-A library with an Illumina TruSeq Stranded mRNA Sample Preparation kit according to standard protocol. Paired-end 125 nt sequencing was performed by MGX-Montpellier GenomiX platform on an Illumina HiSeq 2500.
GEO Accession
GSM2807427