RNA-Seq of homo sapiens: BDCA1+DC from primary luminal breast tumor
RNA from sorted cells (>100 cells) was extracted by using Single Cell RNA Purification Kit, including on-column DNase digestion. cDNA was generated with SMARTer Ultra Low input RNA for Illumina Sequencing-HV. 14 cycles were used to amplify cDNA. Quantity and quality of cDNA was assessed with Qubit dsDNA high sensitivity and Agilent Bioanalyzer using nanochip respectively. Multiplexed pair-end libraries 50nt-length, were obtained using Nextera XT kit. Sequencing was performed in a same batch in Illumina HiSeq 2500 using 15 million read depth, 50nt-length reads per samples were obtained.