16S rRNA seawater BrdU-labelled prokaryotes 2222m
Incubated 24h with 20nM (final concentration) bromodeoxyuridine. 4L filtered seawater. DNA extracted consecutively with phenol pH8, phenol:chloroform:isoamyl alcohol 25:24:1 and chloroform. BrdU-labelled DNA isolation through magnetic bead immunocapture. 16S amplification using primers 515F-Y and 926R