RNA-seq of microvesicles extracted from K562 cells
The human chronic myeloid leukemia blast crisis cell line K562 were cultured in RPMI 1640 containing 15% fetal bovine serum at 37°C in 5% CO2. For microvesicles (MVs) isolation, K562 cells were centrifuged at 1000 ×g for 10 min. The supernatant was centrifuged at 5000 ×g for 20 min to remove cellular debris, and the remaining supernatant was centrifuged at 13000 ×g for 60 min to obtain MVs. Total RNA was isolated from K562-MVs using Trizol reagent (Invitrogen Life Technologies, Carlsbad, CA, USA) and performed RNA-seq using HiSeq2000 at WuXi AppTec (Shanghai, China).