Illumina HiSeq 2000 sequencing; miRNASeq of musculoskeletal ageing using human mesenchymal stem cells and their tissue constructs
miRNASeq of musculoskeletal ageing using human mesenchymal stem cells and their tissue constructs
MSCs purchased from young and old donors grown in osteocenic media for 21 days grown in pellet culture in chondrogenic media grown as 3D tendonconstructs in tenogenic media miRNAeasy columns for RNA purification following Trizol treatment New England BioLabs (NEB) NEBNext Multiplex Small RNA Library Prep Set for Illumina, according to manufacturer’s instructions
Experimental Factor: age
young
Experimental Factor: clinical information
mesenchymal stem cell
LIBRARY_STRAND
second strand