GSM2732736: B6_old_mtAKR_rep1; Mus musculus; RNA-Seq
Total RNAs were extracted from liver samples obtained from conplastic mouse strains (C57BL/6J and C57BL/6J-mtAKR/J), using innuPREP RNA Mini Kit (Analytik Jena) according to the manufacturer’s protocol. RNAs quality was checked using a Bioanalyzer system (Agilent Genomics). RNA sequencing of primary samples was carried out employing the TruSeq  stranded Total RNA kit (Illumina Inc.), starting from 0.6µg total RNA of  each sample and sequenced on an Illumina HiSeq4000 (75-nucleotide  paired-end  reads). Aiming for high transcriptome coverage, sequencing runs were  performed out combining 4 samples per lane (corresponding to approximately  50 million reads per sample).
GEO Accession
GSM2732736