GSM3825599: WT ESC polyA RNAseq repl3; Mus musculus; RNA-Seq
RNA from WT ESCs were extracted with either the RNeasy Mini or Maxi kit or TRIzol according to the manufacturer's instructions. Genomic DNA was eliminated by DNase treatment. Poly-A enriched mRNA sequencing was performed on the Illumina Hiseq 2000 platform following library preparation using the TruSeq Stranded mRNA Library Prep Kit (which includes the polyA-enrichment step). 100-200M strand-specific pair-end reads of 150bp were generated per sample.