EdUseq-HU of U2OS cells expressing normal levels of cyclin E: 14 h after mitotic shake-off treated with DRB for the first 7 hours of G1
U2OS cells expressing normal levels of cyclin E were synchronized by mitotic shake-off and released into media containing HU for 14 h before being collected. DRB was added for the first 7 h after mitotic exit. EdU was present for the last 9 h. The EdU labeled DNA was then isolated and sequenced.