GSM3593789: 4_nhr69Test.GeneExp; Caenorhabditis elegans; RNA-Seq
RNAiso-G (Segenetic, Borken, Germany) was added to the samples, before they were frozen in liquid nitrogen. After thermal disruption of worms (liquid nitrogen, 35°C; three repetitions), chloroform extraction on ice (10 min), and centrifugation (12.000 × g, 4°C, 15 min), DNA was digested and RNA purified with a RNase-free DNase set and RNeasy® mini kit (Qiagen, Hilden, Germany). Quality control was performed using an Agilent Bioanalyzer® (Agilent Technologies, Böblingen, Germany). After adding the samples to RNAstableTM matrix (Biomatrica, San Diego, CA, USA) and a subsequent vacuum centrifigution for drying (2 h), the samples from three experimental approaches were sent to the Beijing Genomics Institute (BGI) for RNA-Seq analysis. RNA libraries were prepared for sequencing using standard Illumina HiSeq2000 protocols.
GEO Accession
GSM3593789