WGS of Oryza Sativa: Leaf
Whole genome DNA shotgun paired end sequencing library was done through DNA fragmentation of about 500bp with DNA shearing machine Covaris (Covaris, CA, US), paired-end adapter ligation of about 53bp and 68bp for sequencing available on the machine FlowCell, size selection of the adapted ligation of the fragments within the desired size range 500~600bp and PCR enrichment using the complete primer constructs required for binding and clustering on the FlowCell.