GSM1666897: ZLW01; Camellia sinensis; miRNA-Seq
Total RNA was prepared from leaves and roots using the Total RNA Purification kit (Norgen Biotek Corporation, Canada), following the manufacturer’s instructions using the on-column DNA contamination removal protocol. A portion of the RNA samples for all tissue samples were mixed into a single RNA pool by equal fraction ratio. The quality and integrity of the total RNA were examined by using the Agilent Technologies 2100 Bioanalyzer (Agilent Technologies, Palo Alto, USA) The small RNA fragments (16-30 bases) were isolated from the 200 l total RNA pool using a 15% polyacrylamide denaturing gel. After purification, the small RNAs were ligated sequentially to RNA 5’ adapters and 3’ adapters, reversely transcribed to cDNA, and amplified by PCR. Finally, the purified and validated sRNA-derived cDNA library was sequenced by Solexa sequencing technology using an Illumina GAIIX system provided by LC Sciences (Houston, Texas, USA)
GEO Accession
GSM1666897