ddRAD sequencing of Aspergillus flavus: strain IC5770
Whole genome DNA extracted from the A. flavus strain. Digested with restriction enzymes MluCI and MspI. Adapters with barcodes were ligated to the sample, and amplified to introduce Illumina adaptor sequences. The sample was sequenced using 125 bp paired end reads on the NextSeq 500 Illumina platform. The sample was demultiplexed based on the barcodes, quality trimmed with Trimmomatic, and aligned to the A. oryzae RIB40 reference genome using the MEM algorithm in BWA.