Illumina MiSeq paired end sequencing; SuhB associates with Nus factors to facilitate 30S ribosome biogenesis in Escherichia coli
SuhB associates with Nus factors to facilitate 30S ribosome biogenesis in Escherichia coli
Overnight cultures were started from single colonies in 2 mL LB at 37 C, shaking at 225rpm.  Subcultures were set up at 1:100 in 20 mL LB and grown at 37 C, shaking at 225rpm until OD600 reached 0. products were sequenced to identify the presence, if any, of suppressor mutations. Genomic DNA from a strain with wild-type rnc was prepared using a DNeasy Blood and Tissue Kit (Qiagen). Nextera kit (Illumina)
Experimental Factor: thyA suhB::thyA
genotype
Experimental Factor: deletion suhB
genetic modification