GBS of HCCBC_1
We sequenced 1376 hybrid individuals descending from an original                 backcross (H. a. annuus x H. debilis F1 was crossed to H. a. annuus) mimicking the                 genetic origin of H. annuus texanus. These were drawn from lineages evoloving in the                 field in four geaographic locations with 12-72 individuals (average at 48) sequenced                 per location and generation. Sixteen genotyping-by-sequencing libraries were                 constructed following the two enzymes protocol. 100ng of genomic DNA was digested                 with the endonuclease PstI-HF and MspI. Fragments were ligated to adaptors including                 the Illumina sequencing adaptor and size selection was performed to retain amplified                 fragments of length between 300-500bp. To reduce the representation of repetitive                 sequences in the libraries, we performed a depletion step by treating the enriched                 libraries with Duplex-Specific Nuclease. Pair-end sequencing 125bp was performed on                 an Illumina HiSeq v4. This data is currently unpublished. These data aim to show the                 potential for repeated evolution in the field in an experimental hybrid lineage with                 H.annuus texanus as the model. The four geographic locations were chosen to                 represent different microenvironments within the range of H. a.                 texanus.