GSM1240814: GP5dControl_H3K4me1_Rabbit; Homo sapiens; ChIP-Seq
Cells were cross-linked by 1% formaldehyde, and DNA was sonicated to 200- 400 bp fragments. Antibodies (5 µg) were added and collected using protein G Sepharose (GE). Cross-links were reversed and proteins digested by incubation with proteinase K at 65 °C overnight. DNA was then purified using Qiagen PCR purification kit.
GEO Accession
GSM1240814