GSM1666129: KM2_CD133_neg_NANOG_IP; Mus musculus; ChIP-Seq
Four pairs of TICs and Nanog-/CD133-/CD49f+ control cells (~1x10^5 per mouse) were isolated from four independent mouse liver tumors. ChIP was performed with NANOG antibody using CD 133+ as well as CD133– cell lines following a standard protocol as suggested by manufacturer (Millipore). In parallel, isotype control antibody was used as a control. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit. Briefly, DNA was end-repaired and blunt, phosphorylated ends were treated with Klenow fragment and dATP to yield a protruding 3- 'A' base for ligation of Illumina's adapters.  After adapter ligation DNA was PCR amplified with Illumina primers for 15 cycles and library fragments of ~250 bp (insert plus adaptor and PCR primer sequences) were band isolated from an agarose gel. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Illumina Hi-Seq  following the manufacturer's protocols.sequences) were band isolated from an agarose gel. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols.
GEO Accession
GSM1666129