Genome sequencing of JLB162
Genomic DNA was diluted to 0.2 ng/ l and paired-end libraries were prepared using the Illumina Nextera XT DNA Library Prep Kit (FC-131-1024, Illumina) according to manufacturer s instructions. Library quality was assessed on the Agilent 2100 Bioanalyzer (Agilent) using a High Sensitivity DNA Chip (5067-4626, Agilent). As all libraries were of high quality and concentrations were > 15 ng/ l samples were normalized and pooled using the bead based method according to the Illumina protocol (Document # 15031942 v02, Illumina). Libraries were sequenced on the Illumina MiSeq (Illumina) using the MiSeq Reagent Kit v2 (500-cycles) 2 x 250 bp paired-end sequencing (MS-102-2003, Illumina). FastQ files were generated and retrieved from BaseSpace Sequencing Hub.