a15
Indexed libraries were constructed from 1000ng of total RNA using a TruSeq Stranded mRNA Sample Prep Kit (Illumina) following the manufacturer s instruction. The quantity and quality of the libraries were also assessed by Qubit and Agilent 2100 Bioanalyzer, respectively and the average library size was 400 bp. Libraries molar concentration were validated by qPCR for library pooling. Sequencing was performed on the Illumina HiSeq 4000 platform using PE150 chemistry (Illumina)