GSM4160897: N720_S508_5 scRNAseq; Homo sapiens; RNA-Seq
Libraries were prepared by getting mRNA be reverse transcribed from an anchored oligo-dT primer that includes a universal priming site at the 5' end. RT reactions also included a template-switch oligonucleotide to allow the incorporation of the universal priming site at cDNA 3' ends. Whole transcriptome cDNA was then amplified by PCR for 22–24 cycles using universal priming sites primers and Kapa HiFi Hot Start ReadyMix. After post-PCR clean-up, amplified whole transcriptome cDNA was incorporated into barcoded Illumina Nextera libraries and sequenced to a depth of about 2 million 150bp paired-end reads/cell on a HiSeq 4000 (Illumina).  and amplified for 14 PCR cycles. Libraries were pooled and paired-end sequenced (38bp+37bp).
GEO Accession
GSM4160897