RNA Editing in Bone tissue of DO Mice
Poly(A) RNA-seq libraries were constructed by the Genome Technologies core facility at The Jackson Laboratory using the TruSeq RNA Library Prep Kit v2 (Illumina). Libraries were checked for quality and concentration using the DNA 1000 LabChip assay (Agilent Technologies) and quantitative PCR (KAPA Biosystems), according to the manufacturers instructions. Libraries were pooled and sequenced by the New York Genome Center, 100 bp single-end on the HiSeq 2500 (Illumina) using TruSeq SBS Kit v4 reagents (Illumina).