16S rDNA V1V3 amplicon sequencing of human respiratory microbiome: nasopharynx
Human respiratory swab was collected from healthy human subject and stored at -80°C and extracted with QIAGEN DNeasy Blood&Tissue kit. 16S rDNA V1V3 amplicon library prepared with DNA extract and 454 fusion primers barcoded with 454 MIDs was sequenced on Roche 454 FLX+ system. Reads were used to human microbiome comparative analyses.