GSM2552185: P14 Iso 1; Mus musculus; RNA-Seq
Cells were isolated by flow cytometry, gating on Thy1.1+ CD8+ cells and collecting live cells. RNA isolation from sorted cells was performed using the PicoPure RNA Isolation Kit (Thermo Fisher Scientific, Waltham, MA). RNA integrity was analyzed using Agilent RNA Nano Kit (Agilent, Santa Clara, CA). Amplified cDNA was prepared using SMART-Seq v4 Ultra Low Input RNA Kit (Clontech, Mountain View, CA), sheared using Covaris tubes (Covaris, Woburn, MA) and Illumina libraries constructed using the NEBNext Ultra DNA Library Prep Kit (NEB, Ipswich, MA) and sequenced using the NextSeq 500 system (Illumina, San Diego, CA) at a depth of 20-25 million single-end 75 bp reads per sample.
GEO Accession
GSM2552185