GSM4160131: miR-373 expressing cells rep 3 (batch 2) [PRO-seq]; Homo sapiens; OTHER
The cells were permeabilized to prepare nuclei. The nascent RNA generated using biotin-run-on reaction of nuclei was used for library preparation. The biotin-labelled RNA was extracted using Trizol, hydrolyzed using NaOH and enriched using streptavidin beads. 3' adapters were ligated and 5' ends were modified, followed by 5' adapter ligation. For batch-2, the 3' adapters containing sample identifiers (allowing to pool libraries after 3' adapter ligation) were used, and both 3' and 5' adapters contained unique molecular index (UMI) at the ligation junction. Purified and adapter ligated biotin-labelled RNAs were reverse transcribed and PCR amplified, followed by size selection and sequencing.
GEO Accession
GSM4160131