De novo characterization of ZHJ1 biotype whitefly transcriptome
mRNA was purified from 6 ug of total RNA. Following purification, the mRNA is fragmented into small pieces and the cleaved RNA fragments were used for first strand cDNA synthesis using random primers. This was followed by second strand cDNA synthesis. These cDNA fragments then went through an end repair process and ligation of adapters. These products were purified and enriched with PCR to create the final cDNA library.