RNA-Seq:SH-SY5Y:Ctl6h_6
Total RNA with an RNA Integrity Number above 9.5 was used to construct libraries using the Illumina TruSeq Stranded mRNA Sample Kit. mRNA was enriched using oligo dT-attached magnetic beads, fragmented, and converted into cDNA. Fragments of cDNA went through an end repair process, 3' ends were adenylated, universal bar-coded adapters were ligated, and cDNA fragments were amplified by PCR to yield the final libraries.