GSM3904434: RNA-seq in HP1g -/-, PMA-treated MEF-derived cell line, replicate 2; Mus musculus; RNA-Seq
Total RNA was extracted by guanidinium thiocyanate-phenol-chloroform extraction (AGPC) followed by proteinase K and Dnase I treatment. Total RNA library preparation was performed on DNase-treated RNA samples by Novogene Co., Ltd, as a lncRNA sequencing service, including lncRNA directional library preparation with rRNA depletion (Ribo-Zero Magnetic Kit), quantitation and pooling. Sequencing (100M raw reads/sample) was performed on the Illumina Hiseq2500 platform to generate paired-end 150 bp reads bearing strand specificity.
GEO Accession
GSM3904434