Illumina HiSeq 2000 paired end sequencing; Transcriptomic profiling of Arabidopsis DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A (AtPOL2a) and B (AtPOL2b) mutants
Transcriptomic profiling of Arabidopsis DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A (AtPOL2a) and B (AtPOL2b) mutants
Seeds were germinated and grown on vermiculite containing soil and maintained at 25oC in 60% Relative Humidity with a day-night cycle of 16/8 h light/dark in controlled environmental conditions. Total RNA was extracted using the RNeasy® Plant Mini Kit (Qiagen, UK) according to the manufacturer’s protocol. The cDNA libraries were constructed in accordance with the Illumina TruSeq RNA sample preparation guide v2 for Illumina paired-end multiplexed sequencing by Source Bioscience, UK.
Experimental Factor: genotype
AtPOL2b mutant (T-DNA)