GSM1234529: tax-Post-R3; Homo sapiens; RNA-Seq
Total RNA was extracted from pre- and post-AAT samples using the RNeasy Mini Kit (QIAgen, 74104) according to manufacturer's instructions.  RNA quantity and quality was evaluated by spectrophotometry using the NanoDrop 2000 spectrophotometer (Thermo Scientific) and 2100 Bioanalyzer (Agilent) RNA electropherograms, with calculation of RNA integrity number (RIN) (35).  Samples were included for RNA sequencing if RIN > 6 and total RNA > 500 ng Samples were processed using the Illumina RNA-Seq protocol (Illumina) according to manufacturer's instructions without poly(A) mRNA selection.  The cDNA sample library was subsequently normalized using the Illumina DSN (Duplex-specific Nuclease) protocol according to manufacturer's instructions.
GEO Accession
GSM1234529