16S rDNA of bacterial community in anerobic bioremediation of tannery chromium wastes
Libraries of bacterial 16S rRNA gene fragments were amplified from each DNA extract using PCR with universal bacterial primers 27F and 1492R, followed by a second nested PCR using 27F and 518R primer pairs having overhang adapter sequences compatible with Illumina indexes and sequencing adapters . Resultant PCR products were cleaned, indexed the library, before pooling and sequencing on Illumina Miseq 250 to generate paired