GSM3525696: W83-Gal-grown-3; Porphyromonas gingivalis W83; RNA-Seq
The Direct-zol RNA MiniPrep Kit (Zymo Research) kit was used for RNA extraction. Samples were next treated in two separate rounds with DNase I to digest gDNA. After washing the column, total RNA was eluted using nuclease free water. This elution was repeated a second time to concentrate samples. Only samples with an RNA integrity number (RIN) of 7.0 or greater were processed. The Illumina Ribo-Zero magnetic kit for bacterial RNA was used to reduce ribosomal RNA. 5 μL of samples were fragmented and used to perform first strand synthesis with random priming. The NEBNext Ultra RNA library prep kit for Illumina (New England Biolabs) was used to prepare libraries. Samples were enriched by rounds of PCR and purified using the Agencourt AMPure XP system.
GEO Accession
GSM3525696