Gordius sp. transcriptome
RNA was extracted and purified from RNAlater-preserved or frozen tissue samples using the Omega Bio-Tek Mollusc RNA kit or the Qiagen RNeasy kit. In either case, an on-column DNAse digestion was performed. RNA concentration was measured using a Qubit (Thermo Fisher) with the RNA High Sensitivity kit, RNA purity was assessed by measuring the 260/280nm absorbance ratio using a Nanodrop Lite (Thermo Fisher), and RNA integrity was evaluated using a 1% SB agarose gel or a TapeStation (Agilent). At least 1g of total RNA for each specimen was sent to Macrogen (Cambridge, MA, USA) for Illumina TruSeq RNA v2 library preparation (polyA enrichment) and sequencing on the Illumina HiSeq 2500 system using the HiSeq SBS V4 chemistry with 100bp paired-end sequencing.