GSM4315355: 101-027EOS; Homo sapiens; RNA-Seq
Around 100 sections were cut from each PAXgene-fixed paraffin block. RNA extraction was carried through PAXgene Tissue RNA kit (QIAGEN #765134) and QIAGEN QIAcube (#9001885) automated extracion system. The library preparation was done using the QIAseq UPX 3' Transcriptome Kit (QIAGEN). A total of 10ng purified RNA was converted into cDNA NGS libraries. During reverse transcription, each cell is tagged with a unique ID and each RNA molecule is tagged with a unique molecular index (UMI). Then RNA was converted to cDNA. The cDNA was amplified using PCR and during the PCR indices were added. After PCR the samples were purified. Library preparation QC was performed using TapeStation 4200 (Agilent) or Agilent Bioanalyzer. Based on quality of the inserts and the concentration measurements the libraries were pooled in equimolar ratios. The library pool(s) were quantified using qPCR. The library pool were then sequenced on a NextSeq500 sequencing instrument according to the manufacturer instructions.
GEO Accession
GSM4315355