GSM3595659: TCR_spleen_01; Mus musculus; RNA-Seq
Total RNA was extracted with the RNeasy Mini Kit Plus (Qiagen, Valencia, CA, USA). Total RNA was converted to complementary DNA (cDNA) with Superscript III reverse transcriptase (Invitrogen, Carlsbad, California, USA). Then, double strand (ds)-cDNA was synthesized and an adaptor was ligated to the 5′ end of the ds-cDNA and cut with SphI restriction enzyme. For TCRβ, PCR was performed with a P20EA adaptor primer and a TCRβ-chain constant region-specific primer (mCB1). The second PCR was performed with mCB2 and P20EA primers using the same PCR conditions. After Tag PCR amplification, index (barcode) sequences were added by amplification with a Nextera XT Index Kit v2 setA (Illumina, San Diego, California, USA). Sequence was done with the Illumina MiSeq paired-end platform (2 × 300 base pairs (bp)).
GEO Accession
GSM3595659