GSM4412256: C2C12 H3K4me3 (primary-Ab ChIL) at 100 cells replicate 2; Mus musculus; ChIP-Seq
Immunostaining half of the protocol includes cell dispensing, fixation, permeabilization, blocking, reaction to primary antibody and the ChIL probe. The reactive probe are integrated into a proximal genomic region to where the primary antibody is bound upon Tn5 activation. The integrated ChIL DNA is used for the linear amplification of genomic sequences and the transcribed RNAs are utilized for library preparation for deep-sequencing.
GEO Accession
GSM4412256