RNA-Seq of human: colonic epithelium
Sequencing libraries were generated from 250  1000 ng total RNA using the TruSeq RNA Sample Preparation kit (Illumina, San Diego, CA) following the manufacturers instructions and quantified using a Library Quantification kit (Kapa Biosystems, Wilmington, MA) as per manufacturers instructions.  The libraries were pooled and sequenced on an Illumina HiSeq 2500 at Texas AgriLife Genomics and Bioinformatics core (College Station, TX).