RNA-seq of Arabidopsis thaliana: UV-B response of wt vs. tga2/5/11
Quality of total RNA isolated for library preparation was determined with Fragment Analyzer (Advanced Analytical Technologies, Cat DNF-471). All samples used for library preparation had a RNA Quality Number (RQN) 8 or superior. Library preparation for RNA-seq was performed using the TruSeq RNA Sample Preparation Kit (Illumina, Cat. RS-122-2102) starting from 2 ug of total RNA and following the manufacturers instructions. Library quantitation was performed by qPCR using the Library Quant Kit Illumina GA (KAPA, Cat KK4824). The size range of the libraries was determined using the High Sensitivity NGS Kit on the Fragment Analyzer (Advanced Analytical Technologies, Cat DNF-474). The libraries were clustered on-board and sequenced by using the HiSeq2500 in order to generate 125b PE reads (Illumina, Cat PE-402-4002, FC-402-4021and FC-402-4022).