GSM2884381: hESCs_2days_rapamycin_rep1; Homo sapiens; RNA-Seq
Treated cells were harvested two or four days after rapamycin treatment. Total RNA was isolated from three independent biological replicates with RNeasy Mini Kit (QIAGEN). mRNA fraction of total RNA was enriched by pulldown of poly(A)-RNA (1ug per sample). RNA sequencing libraries were generated using the Illumina TruSeq RNA prep kit v2 according to manufacturer's protocol and sequenced using Illumina NextSeq 500 with 84 bp single-end reads.
GEO Accession
GSM2884381