GSM1238999: OX-Empty + IPTG, 12 hours; Synechococcus elongatus PCC 7942; RNA-Seq
Total RNA was prepared from extracted cells using the hot phenol/SDS method as described previously (Vijayan, V., et al. (2009). Proc Natl Acad Sci U S A 106, 22564-22568). Ribosomal RNA was depleted from 6 μg of total RNA using the MICROBExpress Bacterial mRNA enrichment kit (Applied Biosystems) according to manufacturer’s instructions. Strand-specific RNA-sequencing libraries were prepared from 375 ng of rRNA-depleted RNA using the TruSeq Stranded mRNA Sample Prep Kit (Illumina). Samples were multiplexed and sequenced on an Illumina HiSeq machine by the core facility at the Harvard FAS Center for Systems Biology.
GEO Accession
GSM1238999