Rht-D1 BAC clone
The reduced height (Rht) locus Rht-D1c, which is a duplicated region of the Rht-D1b gene, confers an extreme dwarf phenotype in common wheat (Triticum aestivum). However, other than the status of segmental duplications, the sequence of duplicated Rht-D1b genes is unknown. In this study, bacterial artificial chromosomes (BACs) were constructed from a dwarf hexaploid wheat Lumai 15 that carries the Rht-D1c locus. BAC clones were identified using specific primers for Rht-D1b and were sequenced separately on a MiSeq platform.