GSM4411822: num_2_subpop_2; Mus musculus; RNA-Seq
Spleens were crushed and filtered with a 70µM mesh strainer. Harvested splenic B-cells were enriched by CD43 negative selection using MACS LS Columns (Miltenyi Biotec). Single-cell suspensions were stained with antibodies and sorted by flow cytometry. RNA was extracted from the sorted samples by RNAeasy Micro Kit (#74004, QIAGEN, for cMyc+ GC-B cells). Libraries were prepared with NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina®
GEO Accession
GSM4411822