GSM3673906: RPE 1 hTert: 300nM PTX 24h rep 2; Homo sapiens; RNA-Seq
Total RNA was collected and purified using the PureLink RNA Mini Kit (Invitrogen, Thermo Fisher Scientific, USA). RNA concentration and quality were determined using NanoDrop and Bioanalyzer respectively 500 ng of purified RNA was used as input for the Illumina TruSeq Stranded mRNA Library Prep Kit (Illumina, USA). Barcoded libraries were pooled and sequenced by The Bauer Core Facility at Harvard University, where the pooled library was quantitated using KAPA and single-end sequenced on an Illumina NextSeq (Illumina, USA).
GEO Accession
GSM3673906