GSM2886894: OE Rif1-MNase 5'; Saccharomyces cerevisiae; ChIP-Seq
DNA was extracted with MasterPure Yeast DNA purification Kit (Epicentre), dissolved in 50ul and treated with RNAse RNAse A 200ug/ml for 20min at 37°C. ChEC DNA was subjected to Ampure XP beads (Agencourt) size selection (amount of beads: 2.5:1 v/v). The un-bound fraction (supernatant) containing the small fragments was recovered Library preparation was carried out using the NEBNext® Ultra™ DNA Library Prep Kit for Illumina protocol kit (Biolabs)
GEO Accession
GSM2886894