Whole metagenome shotgun sequencing of sediment sample D18
DNA extraction was performed from 0.5 g of surface sediment, using the PowerSoil DNA Isolation Kit (MO BIO-QIAGEN) according to the distributor indications. DNA extraction was performed from Sterivex or Millipore filters using the Power Water DNA isolation kit (MO BIO-QIAGEN) according to the distributor indications. Illumina sequencing libraries were prepared using the TruSeq DNA PCR-Free HT Library Prep (Illumina) kit. Sequencing of paired-end reads was carried out in an Illumina NextSeq500 platform with a configuration of 150 cycles by side.