GSM3526474: BaumsDatabaseID_12676 [16S]; Acropora palmata; OTHER
The hypervariable V1 and V2 regions of the 16S rRNA gene were amplified using a general bacterial primer pair with Illumina CS1 and CS2 Fluidigm adapters. [27F 5'-TCGTCGGCAGCGT CAGATGTGTATAAGAGACAGAGAGTTTGATCMTGGCTCAG-3' and 355R 5'-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGCTGCCTCCCGTAGGAGT-3'] (Rodriguez-Lanetty et al. 2013). Polymerase chain reactions were performed using 0.1 U µL-1  Gotaq (Promega), 1X Gotaq buffer (Promega), 0.25 mM dNTPs (Bioline), 2.5 mM MgCl2, and 0.25 µM of each primer.   Initial denaturation was performed at 95°C for 5 min followed by 30 cycles of 95°C for 30 sec, 51°C for 1 min, and 72°C for 1 min.  Final extension was performed at 72°C for 7 min.  PCR products were checked on a 1% agarose gel for a band near 450bp.  Libraries were prepared at the DNA services facility at the University of Illinois, Chicago and sequenced on a MISEQ2500 platform. Genets P1034, P2138, P1002, and P2151 were run on one chip, and Genets P2656 and P2598 were run together on the second Miseq chip.
GEO Accession
GSM3526474