EdUseq-HU/release of U2OS cells expressing normal levels of cyclin E: 14 h HU arrest and release for 90 min.
U2OS cells expressing normal levels of cyclin E were synchronized by mitotic shake-off and released into HU-containing media for 14 h, cells were then released from HU for 90 minutes with EdU being added 30 minutes before collection. The EdU labeled DNA was then isolated and sequenced.