GSM1234293: mDip_mc; Apis mellifera; MeDIP-Seq
meDIP: Shear DNA to ~600bp fragments:  3µg DNA + TE to 130µl in microTUBE. Denature DNA, 125µl (3µg) sheared DNA + 330µl TE. IP and wash: add 51µl 10x IP buffer + 3µl (1µg/µl) antibody; incubate > 2hours at 4C; add 25µl protein G beads; incubate > 2hours; collect beads with a magnetic rack; wash beads with 700µl 1xIP buffer, incubating 10 minutes x RT x 900rpm (or RT rotator); repeat wash 2 more times. The mC antibodies were mouse monoclonal (Active Motif, Inc.) and the hmC antibodies were rabbit polyclonal (Active Motif, Inc.). Immunoprecipitation was with protein G beads (Active Motif, Inc.) following the manufacturer’s protocol.
GEO Accession
GSM1234293