RNA-Seq of human exfoliated colonic cells
Sequencing libraries were generated from 100 ng RNA that had been enriched for poly A tailed mRNA using the Ovation DGE kit followed by the Encore Rapid Library kits from NuGen, Inc (San Carlos, CA) following manufacterer's instructions.  These libraries were quantified using a Library Quantification kit (Kapa Biosystems, Wilmington, MA) as per manufacturers instructions.  The libraries were pooled and sequenced on an Illumina HiSeq 2500 at Texas AgriLife Genomics and Bioinformatics core (College Station, TX).