GSM1665590: late stalk M2G RNA-seq; Caulobacter vibrioides NA1000; RNA-Seq
Cells were lysed by mixer mill using 6 cycles of 3 min at 15Hz.  In between cycles, the chamber was resuspended in liquid nitrogen to keep frozen.  For ribosome profiling: Ribosome protected mRNA fragments were size selected via gel purification. For RNA-seq:  frozen lysate were phenol chloroform extracted at 65C, ethanol precipitated, and sheared by base hydrolysis at 95C.  Resulting mRNA fragments were size selected via gel purification. mRNA fragments were ligated to 5' adenylated DNA oligo. After reverse transcription, the single stranded DNA was circularized, and PCR amplified [Oh et al,. Cell 147, 1295 (2011)].
GEO Accession
GSM1665590