GSM1668624: SF-10-24hr [RMB106]; Mycobacterium tuberculosis CDC1551; RNA-Seq
Treated bacterial cells were treated with RNAprotect Bacteria reagent (Qiagen), followed by extraction of total RNA using RNeasy Minikit (Qiagen) according to the manufacturer’s instructions. rRNA was depleted with Ambion’s MICROBExpress Kit (AM1905) as per the manufacturer’s instructions. RNA libraries were prepared for sequencing using standard Illumina TRUseq version 2 protocols