Novel DPA1 of a Mexican Mestizo female
Isolation of gDNA from whole blood. NGS was performed using Holotype HLA from Omixon. The loci amplified were HLA-A, HLA-B, HLA-C, HLA-DRB1/3/4/5, HLA-DPA1, HLA-DPB1, HLA-DQA1 and HLA-DQB1. Amplicon Quantitation and Normalization was performed using QuantiFluor dsDNA System. Pooling of all loci for each sample into a single tube. Amplicons were enzymatically fragmented, the ends were repaired and adenylated, and indexed adaptors were ligated to the ends. The libraries were pooled and size selected of a single pool of indexed libraries using AMPpure XP magnetic beads. Quantification of the size-selected library and dilution and denature it to 10 pM. Denatured Library was run into the MiSeq for 300 cycles. Sample was analyzed using Omixon HLA Twin software.