Genome sequencing of Halomonas sulfidaeris strain ATCC BAA-803
The strain was cultured using ATCC medium (1097 Halomonas medium, with casamino acids replaced by high molecular weight casein). The liquid culture was passaged two times overnight at 30 C. Genomic DNA was extracted using a Genomic-tip 20/G (Qiagen) and then the size of DNA fragments was checked with TapeStation 2200 (Agilent). Sequencing library was prepared with the Rapid Barcoding Kit (SQK-RBK004) and was subsequently sequenced using R9.4 flow cell (FLO-MIN 106) on a GridION X5 system (Oxford Nanopore Technologies).