RNA-Seq of PD19367
Cells of P. denitrificans were respectively harvested by centrifugation at 5000g for 10 min at 4  after 16 hours exposure to 0 (control ) and 0.75 mg/L TBBPA. By adding TRIzol Reagent (Invitrogen, USA), total RNA of the cells were extracted according to the manufactures instruction, the content and quality of the extracted RNA samples were tested by spectrophotometry (A260/A280) and agarose gel electrophoresis, respectively. mRNA was enriched by removing the rRNA with an Ribo-Zero rRNA Removal Kit for Gram-negative bacteria (Epicentre, Madison, USA). The cDNA library was constructed with the extracted mRNA using a TruseqTM RNA sample prep kit (illumine, California, USA). Sequencing was carried out on the Illumina HiSeq 2000 platform (Majorbio Bio-Pharm Technology Co., Ltd., Shanghai, China).