GSM4160272: S5: MCF10A-scramble shRNA rep2; Homo sapiens; RNA-Seq
Total RNA was isolated from MCF10A/control cells using the RNeasy Plus Mini Kit (Qiagen) following the manufacturer's protocol. RNA-seq libraries were made using the TruSeq Stranded Total RNA LT Sample Prep Kit (with Ribo-Zero™ Human/Mouse/Rat, RS-122-2201) and multiplexed with Illumina barcodes. Two biological duplicates of MCF10A/control samples, HER2 overexpression and two constructs of shRNA PHF8 were sequenced on the HiSeq2500 instrument following protocols supplied by Illumina (San Diego, CA). RNA libraries were prepared for sequencing using standard Illumina protocols. 50-bp paired-end reads were generated. More than 20 Mb reads were produced for each library.
GEO Accession
GSM4160272