Illumina HiSeq 2500 paired end sequencing; Indentification of Dis3L2 targets in Drosophila melanogaster wing imaginal discs
Indentification of Dis3L2 targets in Drosophila melanogaster wing imaginal discs
60 wing imaginal discs per sample were dissected from wandering 3rd instar larvae. 69B-GAL4 homozygous females were crossed to homozygous UAS-dis3L2RNAi males at 25 degrees C.All offspring were of knockdown (UAS-dis3L2RNAi/+ ; 69B-GAL4/+) genotype.  RNA was extracted from each sample of 60 wing discs using a miRNeasy kit.  RNA was polyA selected then libraries were constructed using an illumina TruSeq RNA sample preparation protocol.
Experimental Factor: dis3L2 knockdown
genetic modification
Experimental Factor: w1118 ; P{GD9240}v51854/+ ; P{GaqB}69B/+
genotype