GSM4661737: Flusilazole_GD21_M12c; Rattus norvegicus; RNA-Seq
Fetal testes were removed at gestational day 17 or 21 and stored in RNA-later at -80°C. RNA was harvested using RNeasy Micro Kit (Qiagen, Germany) including on-column DNase I treatment according to manufacturer's instructions. Libraries were prepared from 10 ng of total RNA. Poly(A) tailed mRNAs were tagged with universal adapters, well-specific barcodes and unique molecular identifiers (UMIs) during reverse transcription. Barcoded cDNAs from multiple samples were then pooled, amplified, and tagmented using a transposon-fragmentation approach that enriches for 3′ ends of cDNA. The resulting library of 350-800 bp was sequenced on anIllumina HiSeq 2500 using a TruSeq Rapid SBS kit (Illumina, San Diego, CA, USA).
GEO Accession
GSM4661737