sib-pair sequencing of PBHSC
About 100,00 cells were encapsulated in agarose, genomic DNA was extracted, digested with PacI and DNA fragments 50-90 kb in size were isolated by pulse field electrophoresis as described in Zhang, M. et al. 51 Sequencing libraries with 350 bases inserts were then prepared using the low-input NEBNext Ultra II DNA Library preparation kit and 150 base pair pair-end sequencing was performed on the Illumina platform to a depth of at least 12 Gb per library.