GSM4160473: Brain, F1094_F_HFD; Mus musculus; RNA-Seq
Mice were anesthetized with Avertin (250 mg/kg of body weight, i.p.) and perfused transcardially with cold 0.1 M PBS, pH 7.4: left hemisphere fixed in 4% paraformaldehyde for histology. The cortex was dissected from the other and snap frozen on dry ice for RNA and protein isolation RNA was isolated from cortex and purified using RNeasy mini kit (Qiagen) per the manufacturer's protocol. RNA quality was assessed using 2100 Bioanalyzer (Agilent Technologies) and samples with RIN > 8 were further used for sequencing. Sequencing libraries were generated using mRNA Library Prep Reagent Set (Illumina) according to the manufacturer's recommendations
GEO Accession
GSM4160473