E_macularius_lung01
RNA was obtained from different organs of leopard gecko (Eublepharis macularius). Spleen, bowel and lungs were extracted and processing using 3 mL of RNAlater solution (Ambion, USA) and 0.1mm zirconia/silica beads (Biospec Products, USA). Animal tissues were mechanically disrupted in a high-energy cell homogenizer (Mini-Beadbeater-16, Biospec Products, USA). Total RNA was obtained using a kit for total cellular RNA extraction following the manufacturer’s instructions (QIAmp RNA Blood mini kit, QIAGEN). 10 ng of high quality mRNA from each organ was isolated with Dynabeads RNA direct-Micro kit (Ambion-Life Technologies) and used for library construction (Ion Total RNAseq, Life Technologies). The amplification of cDNA was performed using the kit Ion PI Template OT2 200 kit v3 (Life Technologies). Amplified material was sequenced following the Ion PI Sequencing 200 kit v3 protocol (Life Technologies).