GSM3674121: Lung Alveolar Septa_E16.5-2; Mus musculus; RNA-Seq
For best visibility and imaging of the lung alveoli the OCT slides were washed and briefly dehydrated  prior to LCM using Arcturus® HistoGene® LCM Frozen Cection Ctaining Kit.  Using the ArcturusXT system, lung alveoli samples are collected in CapCure® LCM Caps (#LCM0211) and  stored in lysis buffer (Invitrogen) at - 80°C or flash frozen in liquid N2, and then shipped in bulk on dry  ice to our collaborators for further downstream analysis. More specifically we use the IR laser to select  the alveolar areas, and then cut widely around the selected areas using the UV laser to avoid large blood  vessels and bronchi. For more details please view the SOP here: https://lungmap-sops.s3.amazonaws.com/LMSO0000000066/UAB_tissue_prep_LCM.pdf library preparation was performed  by Illumina® TruSeq® Stranded Total RNA Sample Preparation kits with Ribo-Zero according manufacturer's protocols
GEO Accession
GSM3674121