Illumina HiSeq 2000 paired end sequencing; Transcriptome profiling of Arabidopsis embryogenesis in vitro
Transcriptome profiling of Arabidopsis embryogenesis in vitro
Somatic embryos were induced on B5 medium supplemented with 5 uM 2, 4-dichlorophenoxyacetic acid (Gaj, 2001). In vitro cultures were maintained at 24C under a 16 h/ 8 h (light/dark) cycle in a culture room. Samples were collected after 10 d of in vitro culture. Approximately 700 embryogenic cultures induced at each time-point were pooled together to extract total RNA. Total RNA was extracted using the RNeasy® Plant Mini Kit (Qiagen, UK) according to the manufacturers protocol. The cDNA libraries were constructed in accordance with the Illumina TruSeq RNA sample preparation guide v2 for Illumina paired-end multiplexed sequencing.
Experimental Factor: time after innoculation
10 day