GSM2884066: GW12_PFC1; Homo sapiens; RNA-Seq
Single cells were directly picked into 2.5ul cell lysis buffer the same as Smartseq2 except that we used barcoded reverse transcription primers instead of oligdT30VN primer. Further cell lysis, reverse transcription and amplification was referred to Smartseq2 with little modification. Each cell was amplified for 18 cycles in total. The PCR product for each barcode 1 to barcode 96 was pooled together for purification and library construction. The libraries were sequenced on illumina platform for 1.8M 150bp pair-end reads per cell.
GEO Accession
GSM2884066