GSM3070219: MDA-MB-231p27CK-DD_cJun_ChIPSeq-rep2; Homo sapiens; ChIP-Seq
ChIP assay was preformed by ChIP-IT High Sensitivity kit (Active Motif; 53040). After cells were crosslinked with 1% formaldehyde, the cells were lysed and sonicated. For the immunoprecipitation, sonicated samples were incubated with anti-cJun (Cell Signaling; 9165s) and p27 (Cell Signaling; 3686s) antibodies for overnight at 4°C. After elution DNA, the quality was measured using Bioanalyzer DNA HS (Agilent Technologies). Libraries were prepared using NEBNext® Ultra™ DNA Library Prep Kit (NEB), and their quality was measured by High Sensitivity D1000 Screen Tape (Agilent Technologies). ChIP sequencing was performed on Illumina 75 cycles 400M reads.
GEO Accession
GSM3070219