GSM3070584: 818-8E; Mus musculus; RNA-Seq
QIAshredder (Qiagen; 79656) and RNeasy Mini kit (Qiagen; 74106) were used to isolate RNA from cell lines. The standard protocol of the kit was followed. RNA-seq was performed by New York genome center.  RNA sequencing libraries were prepared using the TruSeq Stranded mRNA sample preparation kit in accordance with the manufacturer's instructions.  Briefly, 500ng of total RNA was used for purification and fragmentation of mRNA.  Following conversion of mRNA to cDNA, DNA was adenylated, ligated to Illumina sequencing adapters, and amplified by PCR (using 10 cycles).  Final libraries were quantified using the KAPA Library Quantification Kit (KAPA Biosystems), Qubit Fluorometer (Life Technologies) and Agilent 2100 BioAnalyzer, and were sequenced on an Illumina HiSeq2500 sequencer (v4 chemistry) using 2 x 50bp cycles targeting 35M single-end reads per sample.
GEO Accession
GSM3070584