GSM3733699: Cre Injected 2; Danio rerio; RNA-Seq
For RNAseq, total RNA was isolated from zebrafish embryos at 6-somite stage using the miRNeasy micro Kit (Qiagen). For exclusion of genomic DNA contamination, the samples were treated by on-column DNase digestion (DNase-Free DNase Set, Qiagen). Total RNA and library integrity were verified with LabChip Gx Touch 24 (Perkin Elmer). 10ng of total RNA was used as input for SMARTer Stranded Total RNA Sample Prep Kit - Pico Input Mammalian (Takara Clontech) following manufacturers protcol. Sequencing was performed on the NextSeq500 instrument (Illumina) using v2 chemistry, resulting in average of 30M reads per library with 1x75bp single end setup.
GEO Accession
GSM3733699