RNA-Seq for gene identification and transcript profiling of three Stevia rebaudiana genotypes
Three different chemical types of Stevia with divergent SG composition from were selected in this study. Routinely, the Stevia leaves were harvested and collected for transcriptome sequencing in the bud stage when the SGs peak. The plant leaves were then cut into small pieces and were immediately frozen in liquid nitrogen. All materials were stored at -80°C until further processing.