GSM1666386: NHEK-D H3K27ac; Homo sapiens; ChIP-Seq
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with the respective histone antibodies. Sequencing libraries were generated for the H3K4me1, H3K27ac, and H3K4me3 samples using the NEB Next reagents and Illumina adaptors and oligos, according to the Illumina protocol for ChIP-Seq library preparation, with some modification. After adaptor ligation, PCR amplification was performed prior to size selection of the library (Schmidt, 2009). Clustering and 50-cycle single end sequencing were performed on the Illumina Hi-Seq 2000 Genome Analyzer. Libraries were sequenced on the Illumina Hi-Seq 2000, with 50bp single end sequencing
GEO Accession
GSM1666386