Long amplicon SMRT sequencing of human: NEK1 edited fibroblast DNA
Long amplicons (4-8  kb) were produced in a multiplexed PCR using PrimeStar GLX Polymerase (Takara  Bio) with DNA extracted from CRISPR-Cas9 edited human fibroblast cells. A  SMRTbell library was constructed using the Template Preparation Kit 1.0  (Pacific Biosciences) according to manufactuerer's instructions. Sequening  was performed on the Sequel system using 3.0 sequencing and binding chemistry  and a 10 hr movie time.