GSM2044727: Control CD4 SP cells, rep2; Mus musculus; RNA-Seq
Total RNA was extracted from purified mouse thymocytes using RNeasy Mini kit (Qiagen), including a DNaseI treatment step. For the analysis of mutant zebrafish, RNA was extracted from 4 dpf embryos using Tri-Reagent (Sigma), treated with DNaseI and re-extracted with Tri-Reagent. RNA quality was determined using the 2100 Bioanalyzer instrument (Agilent) and RNA concentration was determined using Qbit 2.0 Fluorometer (Life Technologies). RNA libraries were prepared for sequencing using the TruSeq Stranded Total RNA kit with Ribo-Zero Human/Mouse/Rat (RS-122-2203, Illumina).
GEO Accession
GSM2044727