GSM2113441: H3K27ac_ChIP-seq_rep2; Mus musculus; ChIP-Seq
Nuclear lysates from cross-linked cells were sonicated to fragment chromatin and histone-DNA complexes were immuno-precipitated with anti-H3K27ac antibody. At least 1ng of ChIP DNA was used for library preparation according to the Illumina ChIP-seq protocol. Briefly, after end-repair and adapter ligation, fragments were size-selected (cut) on an agarose gel prior to pre-amplification and clustering. The size-selected and pre-amplified fragments were verified on a 2100 Bioanalyzer (Agilent, USA) before sequencing on a Genome Analyzer II (Illumina, USA) according to manufacturer’s instructions.
GEO Accession
GSM2113441