Whole metagenome shotgun sequencing of water sample A04 AAIW (1000m)
DNA extraction was performed from Sterivex or Millipore filters using the Power Water DNA isolation kit (MO BIO-QIAGEN) according to the distributor indications. Illumina sequencing libraries were prepared using the TruSeq DNA PCR-Free HT Library Prep (Illumina) kit. Sequencing of paired-end reads was carried out in an Illumina NextSeq500 platform with a configuration of 75 cycles by side.