GSM2883114: GF3; Pseudotsuga menziesii; RNA-Seq
Total RNA was extracted from needles using PureLink Plant RNA Reagent per the manufacturer's protocol (Ambion, Life Technologies, Inc. Austin, TX).  Messenger RNA was purified from total RNA samples with FastTrack MAG mRNA Isolation Kits (Ambion, Inc.). RNA-sequencing libraries were prepared with PrepX RNA-Seq for Illumina Library Kits using an Apollo 324 automated system (Wafergen Biosystems, Inc, Freemont, CA). Clusters were amplified from pooled libraries with TruSeq PE Cluster Kit v3-cBot-HS kits on a cBot System (Illumina Inc., San Diego, CA).
GEO Accession
GSM2883114