GSM3526287: ChIP_input_WT; Mus musculus; ChIP-Seq
MeCP2 ChIP experiments were performed as previously described (Obri et al. 2014) in MEFs WT or KO for Mecp2. Briefly, 50 µg of sonicated chromatin isolated from sub-confluent MEFs WT or KO for Mecp2 were immunoprecipitated using 2 µg of antibody anti-MeCP2 (Proteintech®, 10861-1-AP). Six independent chromatin immunoprecipitations were pooled before sequencing analysis. Libraries were prepared using the Diagenode MicroPlex Library Preparation kit v2, and sequenced on Illumina Hiseq 4000 sequencer as single-end 50 bp reads following Illumina's instructions.
GEO Accession
GSM3526287