GSM4409504: RMC097-SHC_2; Mus musculus; RNA-Seq
ES cells (V6.5) were infected with lentivirus carrying either Non-targeting shRNA or Cnot10 shRNA in the presence of 8 ug/ml of polybrene. 24 hours later, ES cells were selected with 2 ug/ml of puromycin for additional 48 hours and then were grown one passage off feeders for 30 min before harvesting. Total RNA was isolated with the RNeasy (74106 Qiagen) kit, treated with DNase I (M0303L NEB), and re-purified with RNeasy. For total RNA-seq analyses, 2.5 µg of total RNA was depleted of ribosomal RNA with the Ribo-Zero kit from Epicentre (#RZH1046). Ribosomal RNA-depleted samples were used for library preparation with the TruSeq RNA Sample Prep Kit (FC-122-1001 Illumina) for next-generation sequencing. TruSeq RNA Sample Prep Kit (FC-122-1001 Illumina).
GEO Accession
GSM4409504