GSM2886510: MT4_Rosu; Homo sapiens; RNA-Seq
RNA was isolated using the NucleoSpin® RNA/Protein Kit (Marcherey-Nagel, Germany). Treated myotubes were washed and either frozen at -80°C or scraped into the NucleoSpin® RNA lysis buffer. RNA quantity and purity were determined using a NanoDrop ND-1000 spectrophotometer (Thermo Scientific, USA) and RNA integrity using Agilent 2100 bioanalyzer (Agilent Technologies, USA). The RIN factor was >8 for all samples. cDNA libraries for paired-end sequencing were prepared using the TruSeq Stranded mRNA library preparation kit (Illumina, USA). RNA sequencing was performed at Illumina HiSeq platform (Illumina, USA).
GEO Accession
GSM2886510