Transriptome of Lepidoglyphus  destrucor
The concentration of total RNA was determined using the Qubit RNA Assay Kit (Life Technologies). Poly-A selection and library preparation was performed by using the KAPA mRNA HyperPrep Kits (Roche) by following the manufacturer's instructions. 1000 ng RNA was used to prepare the library. Following the library preparation, the final concentration of the library (Table 1) was measured using the Qubit dsDNA HS Assay Kit (Life Technologies), and the average library size was determined using the Agilent 2100 Bioanalyzer (Agilent Technologies). The library was then diluted to 0.6nM, and sequenced paired end for 500 cycles using the NovaSeq 6000 system (Illumina).