GSM4161146: RNA_seq_HNF4a_null_4; Mus musculus; RNA-Seq
A maximum of 500,000 hepatocytes were homogenized in TRIzol Reagent (Thermo Fisher) and incubated at room temperature. Then, chloroform purification (Millipore Sigma C2432) was taking place following by ethanol precipitation. Later, RNA was purified using a RNeasy Mini Kit (Quiagen) and eluted with DDW. RNA was quantified using a NanoDrop-1000 (Thermo Fisher) and RNA quality was measured using a BioAnalyzer RNA Nano Chip (Aligent). Libraries were prepared using an Ultra RNA Library Prep Kit for Illumina (New England BioLabs) with 150 ng of input RNA.  Libraries were sequenced on the Illumina Hiseq 4000.
GEO Accession
GSM4161146