plasma HBV population in patient pair 2 mother
We use tag-linkage method to construct lilbrary. Firstly, we amplified whole HBV reverse transcriptase sequence by high fidelity PCR. There is random nucleotide tags on PCR primers. Then we diluted this product and used another tagged primer to amplify amplicons for sequencing. We used NEB end repair enzyme and ligase to prepare sequencing libraries. Haplotypes were reconstructed from tag information.