Campylobacter jejuni infected and uninfected humanized and murine microbiota mice
Illumina Paired-end sequencing of sample SAMN06624859 METAGENOMIC DNA
The V4 region of the 16S rRNA gene was amplified using dual indexed primers and methods as described by the Patrick Schloss lab. PCR products were normalized using an Invitrogen SequalPrep DNA Normalization plate and the normalized products pooled. After QC and quantitation the pool was loaded on a standard MiSeq v2 flow cell and sequenced with a 500 cycle v2 reagent kit (paired end 250bp)