RNA-seq of mononuclear cells incubated with K562 microvesicles for two weeks
Mononuclear cells (MNCs) were incubated with microvesicles extracted from K562 for two weeks. Total RNA was isolated from cells using Trizol reagent (Invitrogen Life Technologies, Carlsbad, CA, USA) and performed RNA-seq using HiSeq2000 at WuXi AppTec (Shanghai, China).