GSM3732396: O5_Naive_Cranialnerves_rep3; Mus musculus; RNA-Seq
Protocol largely derived from Sanz et al. 2009, PNAS. Each CNS region was microdissected and homogenized in supplemented homogenization buffer (HB-S). Debris was pelleted, and supernatant was immunoprecipitated with an anti-HA antibody (Sigma, #H9658, 1:150) overnight. Following washes, RNA was purifiied using a Qiagen RNeasy purification kit (Qiagen, #74106). mRNA was then isolated using mRNA DIRECT kit (Thermo Fisher, #61012). Libraries were prepared using a similar protocol as MARS-seq (Jaitin et al. Science 2014).
GEO Accession
GSM3732396