R. microplus genomic Cot 69 DNA
Approximately 2 g larvae from the f8 generation of the Deutch strain were used by Amplicon Express Inc. (Pullman, WA) to isolate genomic DNA. Starting DNA concentrations was 200 micrograms of sheared genomic DNA. Time for renaturation was 10 min 49 sec for sample Cot69. Renaturation was conducted at 70 degrees C, at 0.03 M NaPO4. HA was used at 70 degrees to separate ssDNA from dsDNA.