GSM3675936: RNAseq.s2.ctrl11.batch3; Drosophila melanogaster; RNA-Seq
2 million S2 cells or Kc cells after RNAi treatment were resuspended in Trizol and RNA was purified using the RNeasy Mini Kit (QIAGEN). 1 µg of purified total RNA's was used for rRNA depletion using Ribo-Zero Gold rRNA Removal Kit (Illumnia, MRZG 12324) or NEBNext rRNA Depletion Kit (NEB, E6310). Library preparation was done according to the manufacturer's instructions with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7760).
GEO Accession
GSM3675936