Fly-T.b.b infection #1, midgut-derived, SL-primed RNA-Seq
Total RNA was treated with Terminator 5'-monophosphate-dependent exonuclease, followed by DNase I and first strand cDNA synthesis was initiated with random primers. Second strand cDNA synthesis was primed with the spliced leader Primer (5'-GCTATTATTAGAACAGTTTCTGTACTATATTG-3') and platinum Pfx DNA polymerase. double-stranded cDNA was fragmented with DNase I and cDNA fragments corresponding in size to about 200 bp were size-selected on an agarose gel. The cDNA ends were repaired, a single dA was added at the 3' ends and genomic adapters (Illumina, Inc. All rights reserved.) were added. Libraries were enriched by limited PCR and purified on an agarose gel.