GSM3732952: Ribosome-profiling-Siadat2; Neurospora crassa; OTHER
For the ribosome profiling and accompanying mRNA-seq, the ribosome protected fragments (RPFs) and total RNAs were extracted according to ARTseq™ Ribosome Profiling Kit(Catalog Number: RPYSC12116). For the independent mRNA-seq, total RNA was extracted using Trizol reagents (Invitrogen) and treated with DNase (Turbo DNase, Ambion) accoding to standard RNA extraction protocal. For the ribosomal profiling and the accompanying mRNA-seq, libraries were prepared for sequencing according to ARTseq™ Ribosome Profiling Kit(Catalog Number: RPYSC12116). For the independent mRNA-seq, libraries were constucted using Illumina TruSeq reagents. All RNA libraries were prepared for sequencing using standard Illumina protocols.
GEO Accession
GSM3732952