GSM3595615: IgG ChIPSeq; Mus musculus; ChIP-Seq
Lin–cKit+ T cells were purified from WT C57BL/6 mice. The cells were then fixed and sonicated to generate chromatin fragments, followed by immunoprecipitation with an anti-Tle3 antibody or normal IgG, which were then properly washed and immunoprecipitated DNA extracted. The DNA segments from ChIP were end-repaired and ligated to indexed Illumina adaptors, followed by amplification by PCR with a low number of cells (18-21 cycles). The resulting libraries were seqeunced with Illumina Hiseq-2000 platform.
GEO Accession
GSM3595615