GSM3521389: s21-5 noninfected; Gallus gallus; RNA-Seq
Total RNA was isolated using TRIZOL Reagent according to manufacturer's instruction (Life Technologies, Carlsbad, CA). A total amount of 3μg RNA per sample was used as input material for the RNA sample preparations. Firstly, ribosomal RNA was removed by Epicentre Ribo-zeroTM rRNA Removal Kit (Epicentre, USA), and rRNA free residue was cleaned up by ethanol precipitation. Subsequently, sequencing libraries were generated using the rRNA-depleted RNA by NEBNext® UltraTM Directional RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations.
GEO Accession
GSM3521389