MiSeq of KoRV env
Purified DNA was indexed with unique 8 bp barcodes using the Illumina Nextera XT 384 sample Index Kit A-D (Illumina FC-131-1002) following standard PCR conditions. Indexed amplicons were pooled together in equimolar concentrations and sequenced on MiSeq Sequencing System (Illumina, USA) using paired end sequencing with V3 300bp following manufacturers protocols.