GSM3069686: Pfal#3_abdM [RNA-seq]; Procambarus fallax; RNA-Seq
Total RNA was isolated from 20-60 mg frozen tissues. Thawed tissues were homogenized in 1 ml Trizol (Ambion), precipitated with isopropanol and resuspended in 20-100 µl RNase-free water (Gibco Life Technologies). Total RNA was treated with DNase using the RNeasy Mini Kit (Qiagen) following the manufacturer's RNeasy Mini Protocol for RNA Cleanup in combination with the On-Column DNase Digestion Protocol. Sequencing libraries were prepared using 1µg of DNAse-treated total RNA in the first step of the TruSeq RNA Sample Preparation v2 protocol (Illumina, Part# 15026495 Rev. A) as recommended by the manufacturer.
GEO Accession
GSM3069686