GSM3905169: Foreskin fibroblasts, RNA-seq deep senescence rep2; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol (Life Technologies) and mRNA was isolated with the NEBNext Poly(A) mRNA Magnetic Isolation kit (New England Biolabs) Reverse transcription was carried out and cDNA was fragmented, purified, ligated with adapters. PCR was performed using an End Repair/dA-tail ligated DNA template to include unique barcodes to identify each sample.
GEO Accession
GSM3905169