GSM4315336: P4; Mus musculus; RNA-Seq
Total RNA was isolated from tissues using the RNeasy mini kit (Qiagen, Hilden, Germany), following the manufacturer's instructions. RNA concentrations and purity were assessed with the NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific), and RNA integrity was evaluated with the 2100 Bioanalyzer system (Agilent Technologies, Wilmington, DE) using the Agilent RNA 6000 Nano Kit (Agilent Technologies). The RNAseq library was prepared by the Beijing Genomics Institute (BGI; Wuhan, China) using the MGIEasy RNA Library Prep kit (MGI Americas Inc., San Jose, CA, USA). Paired-end reads of 150 bp length were sequenced on the BGISEQ-500RS sequencer (BGI), and raw data were provided by BGI.
GEO Accession
GSM4315336