GSM2552197: KO2_mRNA; Homo sapiens; RNA-Seq
RNASeq libraries were prepared from cells lysed similar to ribosome profiling samples. RNA was isolated from cleared lysates by addition of 1 % SDS (w/v) and 5 min incubation at 65 °C with intermittent vortexing followed by three rounds of extraction using 10:1 acid-phenol pH 4.3 and bromochloropropane. Total RNA was precipitated and 5 µg used for library generation with the TruSeq Stranded mRNA Library Prep Kit (Illumina) according to the manufacturer's instructions. Libraries were sequenced on an Illumina HiScanSQ generating 50 nt single-end reads.
GEO Accession
GSM2552197