GSM3223787: KURAMOCHI_THZ1_50nM_rep1; Homo sapiens; RNA-Seq
KURAMOCHI and COV362 cells were lysed, homogenized using Qiashredder columns (Qiagen), and total RNA was extracted using the RNeasy Plus Mini Kit (Qiagen) according to manufacturer's instructions. Cell count normalized RNA samples were mixed with the ERCC RNA Spike-In Mix #1 (Life Technologies) prior to library preparation. Libraries for Illumina sequencing were prepared using TruSeq Stranded mRNA Library Prep Kit (Illumina) and equimolar multiplexed libraries were sequenced on an Illumina NextSeq 500 (single end 75 bp reads) .
GEO Accession
GSM3223787