GSM2807384: HeLa Oxford Nanopore sequencing 3; Homo sapiens; OTHER
The polyadenylated RNA from HeLa cell was treated with DNase and reverse transcribed with the SuperScript III reverse transcriptase (Thermo Fisher Scientific). PCR were performed with the DreamTaq polymerase (Thermo Fisher Scientific), then purified the amplicons using KAPA pure beads (Kapa Biosystems), quantified by the Qubit fluorometer (Thermo Fisher Scientific). Library for Nanopore sequencing was constructed using Ligation sequencing Kit 1D (SKQ-LSK108) and Native barcoding kit (EXP-NBD103) following Oxford Nanopore Technologies (ONT) protocol.                                 barcode1: AAGAAAGTTGTCGGTGTCTTTGTG;   barcode2: TCGATTCCGTTTGTAGTCGTCTGT;  barcode3: GAGTCTTGTGTCCCAGTTACCAGG;  barcode4: TTCGGATTCTATCGTGTTTCCCTA;  barcode5: CTTGTCCAGGGTTTGTGTAACCTT;  barcode6: TTCTCGCAAAGGCAGAAAGTAGTC;  barcode 7: GTGTTACCGTGGGAATGAATCCTT
GEO Accession
GSM2807384