GSM2732805: 37 degrees C replicate 2; Staphylococcus aureus USA300-0114; RNA-Seq
RNA was isolated from the bacteria using RNAeasy Mini Kit (Qiagen). Quality control of RNA samples was done to determine RNA quantity and quality prior to their processing by RNA-seq. The concentration of RNA samples was determined using NanoDrop 8000 (Thermo Scientific) and the integrity of RNA was determined by 2100 Bioanalyzer (Agilent Technologies). Ribosomal RNA depletion was performed using a Ribo-Zero rRNA Removal Kit (Bacteria) (Illumina, San Diego, CA) according to the manufacturer’s instructions. RNA-seq library generation was continued using the TruSeq RNA protocol (Illumina) starting at the “Elute, Prime, Fragment” step and was completed according to the manufacturer’s instructions.
GEO Accession
GSM2732805