RNA-Seq of Rattus norvegicus: dorsal spinal cord
Sequencing was performed at the NIH Intramural Sequencing Center as described previously24. Briefly, mRNA libraries were constructed from 22 rat dorsal horn samples using 0.6-1  g mRNA and Illumina TruSeq RNA Sample Prep Kits, version 2. The resulting cDNA was fragmented using a Covaris E210 instrument. Library amplification was performed using 10 cycles to minimize over-amplification. Unique barcode adapters were applied to each library. Libraries were pooled in equimolar ratio and sequenced together on a HiSeq 2500 (Illumina, San Diego, CA) with ver 4 flow cells and sequencing reagents. For each individual library, 35-45 million 125-base read pairs were generated.