GSM2551856: Input DNA of DU 145 (cross-linking with 1mM DSP and 1% formaldehyde); Homo sapiens; ChIP-Seq
DU 145 and HEK293T cells were incubateded with 1% formaldehyde only or with a combination of 1mM DSP and 1% formaldehyde. The DNA was fragmented by sonication to approximately 200-300bp or by MNase digestion to obtain a majority of mononucleosomes. UCHL1 was immunoprecipitated by a mouse monoclonal antibody (R&D Systems). The ChIPseq libraries were prepared according to the 5500 SOLiD fragment library protocol (Life technologies). The ChIP and input DNA size was analyzed by the high sensitivity DNA kit and 2100 Bioanalyzer (Agilent Technologies). Approximately 20 ng of ChIP DNA was end-repaired and size selected (100-250 bp). This was followed by dA tailing and ligation of SOLiD barcodes. Finally, DNA libraries were prepared by emulsion PCR (ePCR) and loaded onto the flowchip for sequencing by the SOLiD platform.
GEO Accession
GSM2551856