GSM2045653: Sample_23623_Hyp; Zonotrichia albicollis; RNA-Seq
Brains were quickly removed and flash frozen on dry ice. Brains were cryosectioned at 300um thickness and regions of interest (HYP and MeA) regions were micro dissectied from frozen setions using a 1mm punch tool. A total of 4 punches were collected from each bird and region and were pooled for RNA extraction. RNA was extracted using the Qiagen Allprep DNA/RNA micro kit with modification, as follows. Punches were homogenized in RLT plus + β-mercaptoethanol using a pellet mixer. An equal volume of phenol:chloroform:isoamyl alcohol (25:24:1) pH 7.5 was added to each sample. Samples were further homogenized by passing them through a Qiashredder column (Qiagen). Phases were separated by spinning for 15 min at 4°C, and the aqueous phase was collected and further processed according to the manufacturer’s protocol. Libraries were prepped using Illumina TruSeq RNA Sample Preparation Kit v2. Libraries were constructed using 250 ng total RNA.
GEO Accession
GSM2045653