DNA-seq of Exbucklandia tonkinensis:sapling
The NGSQCToolkit_ v2.3.3 (Patel and Jain, 2012) was used to filtered the raw data by removing the unknown poly N (20% ambiguous base calls) and low-quality reads (more than 20% of nucleotides with Q-value  20). And finally, a total of 34.7 million 100 bp, clean and paired reads were obtained and further de novo assembled into 642,351 contigs with Edena v3.131028 (Hernandez et al., 2008), whose average length is 541 bp and N50 value is 311 bp respectively.