PacBio Sequel Atemnus Politus with LEFT-SEQ Wolbachia capture
LEFT-SEQ (Large Enriched Fragment Targeted Sequencing): large fragments of Wolbachia DNA had been selected with Nimblegen SeqCap EZ DNA biotinylated probes and sequenced using PacBio Sequel sequencing. Circular consensus reads had been determined using only subreads with at least 3 full pass from the insert and predicted accuracy superior at 90.