RNA-Seq of Homo sapiens: adult male normal prostate epithelial cells: Sample RWPE1-Control
"RWPE-1 and WPMY-1 cells (assayed in duplicate) were plated, and 250ng/ml BMP5 (or vehicle control) added with daily media replacement for 72hrs. RNA was isolated using Qiagen RNeasy kit, RNAseq libraries generated using TruSeq Stranded Total RNA LT kit (WPMY-1) and Illumina TruSeq RNA Library Prep Kit v2 (RWPE-1), and barcoded RNAseq libraries sequenced and resultant data processed as for bulk tissue RNA samples."
alignment_software
TopHat/Cufflinks