WGS of soil used for cultivating BSC: bulk soil without BSC growth, taken from the Chicken Creek catchment, sampled at the beginning of a potting experiment, replicate 1
DNA was extracted using the Genomic DNA from soil NucleoSpin Soil Kit (Macherey-Nagel, DE). DNA was sheared using an E220 Focused-ultrasonicator (Covaris, USA). Library preparation was performed using the NEBNext Ultra DNA Library Prep Kit for Illumina and the NEBNext Multiplex Oligos for Illumina (both New England Biolabs, UK). Sequencing was performed on a MiSeq sequencer using a MiSeq Reagent Kit v3 for 600 cycle (Illumina, USA).