GSM3523319: isolated_axon; Xenopus laevis; RNA-Seq
Single Cell RNA Purification Kit (Norgen) was used to extract total RNA from axonal samples following the manufacturer's instruction. In the lysis step, 200µL of the lysis buffer (Buffer RL, Norgen) were mixed with β-mercaptoethanol (Sigma, 1:100), added to the axonal culture and incubated for 5 minutes. RNA was eluted by applying 9µL Elution Solution twice to the column. 10 ng of axonal RNA collected from isolated axons and SPRI beads (Beckman Coulter) purified were used as input for the Ovation SoLo RNA-Seq System, Custom AnyDeplete (NuGEN) . Using the AnyDeplete system Xenopus laevis rRNA sequences identified through the ncbi nucleotide and the SILVA databases were removed.
GEO Accession
GSM3523319