GSM3523434: Gns_2; Homo sapiens; RNA-Seq
Isolated granulosa cells were transferred into lysis buffer. The reverse transcription reaction was performed on the whole-cell lysate and terminal deoxynucleotidyl transferase was used to add a poly A tail to the 3′ end of the first-strand cDNAs, and then 20 cycles of PCR were performed to amplify the single-cell cDNA RNA libraries were prepared according to instructions of Kappa Hyper Prep Kit (Kappa Biosystems).
GEO Accession
GSM3523434