RNA-seq of rattus norvegicus: adult neuropil
Tissue was homogenized in polysome lysis buffer, extract was clarified with high speed spin and 3 aliquots were taken. Aliquot 1: Total ribsosome footprint libraries were prepared according to McGlincy and Ingolia et al. 2017. Aliquot 2:Polysome profiling was performed, monosomes and polysomes were collected and monosome/polysome footprint libraries were prepared according to McGlincy and Ingolia et al. 2017. Aliquot 3: Total RNA-seq library was prepared using the TruSeq stranded total RNA library prep gold kit (Illumina).