RNA-Seq of healthy Labeo rohita at 6day after initiating Aphanomyces invadans- challenge experiment, biological replicate 1
The cDNA sequencing library from total RNA has been prepared following the protocol and using the consumables given with NEBNext ultra directional RNA library prep kit (New England BioLabs, MA, USA). The library was quantified using Qubit fluorometer and its fragment size distribution was analyzed on Agilent 2200 Tape system. Sequencing of library was performed on Illumina HiSeq2500 with 2x100 bp paired-end chemistry to generate sequence reads.