GSM3466927: SC_SC.10; Mus musculus; RNA-Seq
nuclear extraction protocol: 5000-10000 flow cytometry sorted PreCFU-E and CFU-E from Scf FL/FL, Scf KO/FL and Scf KO/FL;Lepr-Cre mice FANS protocol: c-Kit+ bone marrow cells were enriched with anti-APC paramagnetic beads after staining with anti-c-Kit APC-eFluor780 antibody (eBioscience). Cells were then stained with lineage antibodies (CD2, CD3, CD5, CD8, Ter119, B220, Gr1) (Tonbo Biosciences), CD105 (BD Biosciences), CD150 (Biolegend), CD41 (Biolegend), CD16/32 (Biolegend) and Sca1 (eBioscience) total RNA extraction protocol: Total RNA was extracted with RNeasy Micro Kit (Qiagen). Total RNA was extracted following the kit instructions, and RNA integrity and concentration were measure using a Bioanalyzer. Samples with RIN values equal or higher than 9.4 were used for library generation. Two to six ng of total RNA were used to generate a cDNA library using the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara).
GEO Accession
GSM3466927