GSM2731678: DMSO_rep1(PROseq); Homo sapiens; OTHER
Nuclei were isolated by dounce homogenization of treated cells in isotonic buffer containing 0.1% triton-X 100. Nuclear run-on assay was performed with ATP,UTP,GTP and biotin-11-CTP. Nuclei were lyzed in Trizol and nuclear RNA was extracted and fragmented and biotinylated RNA was purified with streptavidin magnetic beads, followed by 3' adapter ligation, 5' repair and adapter ligation, RT and PCR amplification. PROseq
GEO Accession
GSM2731678