GSM3526392: Parental 2; Toxoplasma gondii; ChIP-Seq
Parasites were isolated from infected host cells by syringe-lysis, then nuclear lysates enriched by cellular fractionation were sonicated and clarified by centrifugation. Protein-DNA complexes were immunoprecipitated with antibody-coupled magnetic beads. 1 µg ChIP DNA were used in library preparation using NEBNext Ultra II DNA library prep kit for Illumina (NEB cat#E7645) and the protocol from the manufacturer was followed. A unique 8nt barcode was added to each sample for multiplex sequencing.
GEO Accession
GSM3526392