GSM4160337: KO-5; Mus musculus; RNA-Seq
Whole islets were isolated and total RNA purified using the RNAqueous Kit (Ambion).  Samples were further DNAse treated with the TURBO DNA-free kit (Ambion) as per the manufacturer's instructions.  RNA integrity and concentration was determined by Bioanalyszer and Qubit. PolyA based cDNA libraries were prepared from 8 week islet RNA samples using NEB Next Ultra Directional RNA Library Prep Kit for IlluminaRNA sequencing (E7420S)
GEO Accession
GSM4160337