GSM3215538: reporter_dps_pk_rna_5_sequencing; Drosophila melanogaster; RNA-Seq
Total RNA was isolated from 0.5 million transfected cells. Gene specific cDNA was generated using SuperScript™ II Reverse Transcriptase and primer against GFP. Reverse transcription products were then treated with RNAse A and RNAse H at RT for 30 min and cDNA were purified with Ethanol precipitation. The library preparation procedure for gene specific 5' RNA sequencing is the same as the library preparation for ChIP-nexus, except that a new BamHI cutting oligo and new barcoded primers were used.
GEO Accession
GSM3215538