GSM2552221: Ly-1_PROseq rep1; Homo sapiens; OTHER
Nuclei isolation by douncing in isotonic buffer with 0.1% Triton X-100. Nuclear run-on assays were performed with ATP, UTP, GTP, and biotin-11-CTP or with all 4 biotin-11-NTPs. Nuclei were lyzed with TRIzol, nuclear RNA was extracted and fragmented, and biotinylated RNA was purified with strepavidin magnetic beads, which was followed by 3' adaptor ligation, 5' repair, 5' adaptor ligation, reverse transcription, and PCR full-scale amplification. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM2552221