gonad RNA-Seq of Japanese flounder
RNA-Seq library construction and sequencing were conducted by the sequencing company Novogene (Beijing, China). The cDNA library was prepared in accordance with the recommendations in the manual for NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, USA). The library preparations were sequenced on an Illumina HiSeq 2000 platform with 150 bp paired-end reads.