GSM4261563: C1_S3_Mouse 4hrs untreated; Mus musculus; RNA-Seq
Total RNA was isolated using the RNeasy Micro Kit (Qiagen) as indicated by the manufacturer followed by reverse transcription using the iScript cDNA Synthesis Kit (Biorad).RNA quality and quantity was analyzed on an Agilent Bioanalyzer nano chip. RNAseq libraries were prepared using the stranded TruSeq mRNA kit (Illumina), starting from 500 ng of DNAse I (Qiagen) treated total RNA, following the manufacturer's protocol. The amplified libraries (10 PCR cycles) were purified using AMPure beads (Beckman Coulter), quantified by Qubit 2.0 fluorometer (Life Technologies), and visualized in an Agilent Tapestation 2200. The libraries were pooled equimolarly, and loaded on an S1100 flow cell, and run as paired-end 50 nucleotide reads on a NovaSeq 6000.  Raw paired-end sequencing reads from both human and mouse samples were quality controlled using the FASTQC tool (http://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
GEO Accession
GSM4261563