GBM39 H3K27ac ChIP-seq replicate 1
Formaldehyde crosslinked chromatin from 5 million cells per ChIP was sheared to small fragments by Covaris M220 Focused-ultrasonicator. After pulled down by antibody, and captured by Protein A/G magnetic beads, chromatin was washed 6 times and reverse-crosslinked for 3 hr with RNase A and Proteinase K. ChIP DNA library was constructed by NEBNext Ultra II DNA Library Prep kit for paired-end sequencing.