EdUseq-HU of U2OS cells expressing normal levels of cyclin E: 14 h after mitotic shake-off, treated with DRB for the first 7 h of G1  (replicate 1)
U2OS cells expressing normal levels of cyclin E were synchronized by mitotic shake-off and released into HU-containing media for 14 h with EdU being added for the last 9 h before collection. DRB was present for the first 7 h after mitotic exit. The EdU labeled DNA was then isolated and sequenced.