GSM2113331: A-WT3; Mus musculus; RNA-Seq
Trizol followed by Qiagen RNeasy cleanup and DNase treatment. RNA was measured with a Qubit® (1.0) Fluorometer (Life Technologies) and a Bioanalyzer 2100 (Agilent, Waldbronn, Germany). RNA samples were ribo-depleted using Ribo Zero (Epicentre®, USA). The quality and quantity of the libraries was analyzed by using Qubit® (1.0) Fluorometer and the Caliper GX LabChip® GX (Caliper Life Sciences, Inc., USA). For cluster generation, the TruSeq PE Cluster Kit v3-cBot-HS or TruSeq SR Cluster Kit v3-cBot-HS (Illumina, Inc, California, USA) were used. Paired-end sequencing was performed on an Illumina HiSeq 2000 using the TruSeq SBS Kit v3-HS (Illumina, Inc, California, USA).
GEO Accession
GSM2113331