GSM4263699: Lgr5_2; Mus musculus; RNA-Seq
Intestinal Crypts were collected from Lgr5-WT and Lgr5-CREPTKO mice by pre-cold PBS with 2 mM EDTA and then suspended in TryPLE (Gibco, 12604) to be disaggregated into single cells. Lgr5+ cells were sorted by flow cytometry (BD Arial Ⅲ) based on the fluorescent signals of GFP proteins. Total RNAs were extracted by TriZol (Invitrogen) according to manufacturers' recommendations. RNA libraries were prepared for sequencing using standard BGI protocols
GEO Accession
GSM4263699