GSM2462091: Father 7827; Macaca fascicularis; OTHER
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Illumina TruSeq DNA PCR-Free Sample Prep (550 bp mean insert size) DNA samples (2 ug) were sheared using a Covaris S2 Focused-ultrasonicator and libraries were prepared using the Illumina TruSeq DNA PCR-Free Sample Preparation Kit (cat# FC-121-3001 and FC-121-3002) with an average insert size of 550 bp.  Adapter-ligated molecules were analyzed by quantitative PCR using the Kapa Biosystems Kapa Library Quant Kit (cat#KK4824) and the molarity of individual libraries were normalized to 10 nM in preparation for Illumina sequence analysis.
GEO Accession
GSM2462091