GSM2462860: hCS; Homo sapiens; RNA-Seq
Dissociated single cells from each of hCS and hSS were loaded onto the BD Resolve single cell gene expression analysis system mRNA from individual cells were captured by barcoded oligo probes on a BD Resolve bead. Capture probes contain cell labels (total of 24 bases) and molecule index (8 randomers) such that when cDNAs were extended by reverse transcriptase, each cDNA molecule was tagged with a cell specific sequence and a molecular index sequence. cDNA were then universally amplified following by adaptor ligation. 3' end of transcripts were enriched using random priming and sequenced on Illumina NextSeq.
GEO Accession
GSM2462860