GSM2887531: Rfa2_WT_16dC_ChIP; Saccharomyces cerevisiae; ChIP-Seq
The Rfa2-DNA were isolated with anti-Rfa2 antibody (gift from Dr. Steven Brill's laboratory). DNA prepared above were immobilized on MyOne C1 beads (Life Technology). Primers were added by PCR and adaptors by ligation. Then DNA were eluted as ssDNA at 95°C. The ssDNA were amplified by PCR reaction, and the amount of each sample was verified by qPCR. Verified sample DNA were sequenced by Illumina HiSeq generating about 40bp pair-ended reads.
GEO Accession
GSM2887531