GSM4263916: F508del; colon organoids goblet-enriched; Homo sapiens; RNA-Seq
Total RNA from each sample was extracted in TRIzol reagent (Sigma Aldrich). Total RNA samples were DNaseI treated (5 U DNaseI / 5µg RNA, 30 min, 37°C), spiked-in and rRNA depleted using Ribo-Zero™ Magnetic Gold kit (Human/Mouse/Rat) RNA-size selection and generation of the cDNA libraries was performed as described (Ingolia et al, 2009; doi: 10.1126/science.1168978)
GEO Accession
GSM4263916