2h THY+Hb planktonic cells, replicate #3
For RNA preparation, cell samples were suspended in 700 l of Trizol with 300 mg of  acid-washed glass beads (Sigma Life Science) and disrupted by vortexing.  Total RNA was isolated using the Direct-zol RNA MiniPrep kit (Zymo Research).  DNA was removed using the Turbo DNase-free kit (Life Technologies). rRNA was  eliminated with the Ribo-Zero Magnetic kit for Gram-positive bacteria  (Epicenter). RNA Quality and quantity were assessed using a 2100 Bioanalyzer  (Agilent) and NanoDrop 8000 spectrophotometer (Thermo Scientific), respectively.  Directional RNA-Seq libraries were created using the ScriptSeq v2 RNA-Seq  Library Preparation kit (Illumina) according to the manufacturers  instructions.