Transcriptomic analysis of Meretrix meretrix larvae on different developmental stages
Total RNA were extracted though phenol-chloroform extraction, and mRNA were purified through affinity chromatography. Five microgram cDNA were synthesized through SMART (switching mechanism at 5' end of RNA transcript) cDNA synthesis method and were used for 454 sequencing. To generate enough cDNA, 13 PCR cycles were conducted during the synthesis of cDNA.