mRNA-seq of mus musculus:adult somata
Tissue was homogenized in polysome lysis buffer and lysates were cleared with high speed spin. Ten percent of the lysate were kept as an input. Rpl22-HA immunoprecipitation (IP) was performed on the remaining lysate. RNA was extracted from both, the input and IP. mRNA-seq libraries were prepared using the TruSeq stranded mRNA library prep kit (Illumina).