22_APP/PS1/IL10wt_F
Total RNA was purified from quarter brain whole tissue using Trizol (Invitrogen) and RNeasy columns (Qiagen). Strand-specific libraries were generated with 1 µg RNA input using the TruSeq Stranded mRNA Sample Prep Kit (Illumina) using the low throughput protocol. This includes poly(a) enrichment with oligo dT beads as the first step. Libraries were multiplexed in a pool of 3 using Illumina indexes. Libraries are numbered according to animal number.