Illumina HiSeq 2000 sequencing; RNAseq of primary human gallbladder cells, reprogrammed human gallbladder cells, and human pancreatic beta cells
RNAseq of primary human gallbladder cells, reprogrammed human gallbladder cells, and human pancreatic beta cells
The islets were not cultured. High purity intact human islets were obtained from the Integrated islet distribution program (IIDP).  To isolate the beta cells, islets were enzymatically dissociated into single cells and stained using surface monoclonal antibodies and sorted using FACS following published protocols (pmid:19383399 and27399229). The FACS-sorted beta cells were collected and processed for total RNA isolation. Isolation of RNA from gallbladder cells and beta cells using Qiagen RNeasy protocol TruSeq RNA sample library preparation v2 as recommended by the manufacturer Illumina
Experimental Factor: beta cell
cell type
Experimental Factor: Hpi1 positive
phenotype