GSM2807862: BMM with growing Salmonella Rep 2; Mus musculus; Salmonella enterica subsp. enterica serovar Typhimurium; RNA-Seq
For all mentioned sub-populations 100,000 events were sorted and total RNA was isolated using the mirVana kit (Thermo Fisher Scientific) following the manufacturer's recommendations and DNase I-treated (NEB) for 45 min at 37°C. Generation of cDNA libraries and sequencing was as previously described (Westermann et al., 2016). Briefly, bacterial and murine rRNA was depleted using the RiboZero Gold (epidemiology) kit (Illumina) according to the manufacturer's guidelines. Strand-specific cDNA libraries were prepared by Vertis Biotech. AG (Freising, Germany) and sequenced on the NextSeq500 platform (Illumina)
GEO Accession
GSM2807862