16S rRNA amplicon sequencing to characterize the pelagic microbial community in coastal Monterey Bay
The 16S rRNA gene amplicons were prepared from each sample and sequenced using the MiSeq platform (Illumina; iTag amplicon sequencing), as part of a Community Science Program (CSP) project with the DOE Joint Genome Institute (JGI). Universal (Bacteria and Archaea) V4-V5 primers 515F-Y (5_- GTGCCAGCMGCCGCGGTAA) and 926R (5_- CCGYCAATTYMTTTRAGTTT) (39) were used for amplification.