GSM2552924: Tw2- 2; Mus musculus; RNA-Seq
At 10 days after first dose of TMX treatment, pool of cardiac cells were isolated using Langendorff perfusion. Cells were then further separated by gravity and BSA gradient into cardiomyocytes enriched and non-cardiomyocytes enriched population. Staining of Mitoview633 (Biotium) were performed on non-cardiomyocytes population, then sorted on BD Aria cell sorter based on tdTO and Mitoview633 signal. TdTO+ cells and TdTO- cells were harvested for RNA-seq analysis. Total RNA was extracted using QIAGEN Rneasy mini kit. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM2552924