wild type partheno-sporophyte upright replicate 1
Individuals were cultivated in 320 mm round petri dishes in natural sea water supplemented with 0.01% Provasoli enrichment under low light conditions. Individuals did not produce unilocs or plurilocs. Uprights were dissected by cutting and harvested 4-5 weeks after gamete release (at 16h30 to 18h30 in the evening) and frozen in liquid nitrogen. The cultures used to harvest uprights were not the same as those from which the basal tissue was harvested.