Anaerobic Digester
Sequencing protocolNanopore sequencing was performed by using 1g of DNA as template. The process was initiated by an End repair/dA tailing, Ligation of Barcode Adapter, Barcoding PCR, End repair/dA tailing, Blunt end Adapter Ligation, Purification using AMPure XP bead binding, Priming and loading the SpotON flow cell