GSM3595524: NSC201cb rep2; Homo sapiens; RIP-Seq
Biological replicates (according to ENCODE requirements) of cells were treated with 600mJ of UV using the Stratalinker 2400, harvested in ice cold PBS and pellets flash frozen in liquid nitrogen and stored in -80 degrees until ready to IP with Msi1 bound Dynabeads Subsequent protocol exactly as detailed in van Nostrant et al 2016. Sequencing was performed on Illumina 2000 Hi Seq paired end reads and data processed through Dr. Yeo's laboratory's eCLIP pipeline. Libraries were constructed as per the eCLIP protocol using Illumina 50x forward and 70x reverse primers.
GEO Accession
GSM3595524