GSM4314593: Memory CD4 T cells, F2R2; Mus musculus; RNA-Seq
Lymphoid organs were removed, processed and EYFP+ memory CD4 T cells were sorted and collected into RLT buffer using a FACS Aria IIU cell sorter. RNA was extracted using the Qiagen RNeasy Micro Kit. Briefly, cells were manually homogenized with a pipette and eluted using an RNeasy MinElute spin column. A DNase step was included to remove contaminant DNA and extracted RNA was stored in RNase-free water. Library construction was conducted using an Illumina Hiseq 4000 following the manufacturer's recommended protocol.
GEO Accession
GSM4314593