RNA-Seq of skin of P1(E19.5) WT mice-2
Total RNA was extracted from the skin of wild-type (WT) and Cdc42 KO mice at E17.5 and P1 (n = 2 per genotype for each time point) using TRIzol following the manufacturer s instructions (Invitrogen). An additional DNase I digestion was performed to remove genomic DNA contamination. RNA quality and purity were checked using the NanoDrop (Thermo Fisher Scientific, Inc., USA). Then total RNA samples were used to remove highly abundant ribosomal RNAs before sequencing. The RNA was sequenced on an Illumina Genome Analyzer (Hiseq2000) by Beijing Genomics Institute (Shenzhen, China). Library building and biological information analysis were performed by Total Genomics Solution Company (Shenzhen, China). Low-quality reads were discarded. The low-quality reads included over 50% of the sequences reads (including adapters) with quality scores   10 and n > 5%. Ribosomal RNA sequences were filtered from the raw fragments. The remaining clean reads were aligned to the reference genome using hierarchical indexing for spliced alignment of transcripts (HISAT, http://www.nature.com/nmeth/journal/v12/n4/full/nmeth.3317.html).