GSM4161262: H1299 IKKa knockout xenograft tumor 1_4; Homo sapiens; RNA-Seq
Total cell RNA sample quality and integrity were verified with an Agilent RNA 6000 nano kit or also by quantifying the relative levels of expression of 28S vs. 18S rRNAs by MOPS denaturing agarose gel electrophoresis. RNA-seq libraries were prepared with the Illumina TruSeq RNA v2 kit using 1 μg of total RNA. Libraries were checked with the Agilent bioanalyzer DNA1000 chip, quantified with the Qubit HS spectrophotometric method and pooled in equimolar amounts for Sequencing. 75 bp Single-End reads were generated with the Illumina NextSeq 500.
GEO Accession
GSM4161262