Agropak_3 16S rRNA V3V4 region sequencing
The 16S rRNA sequences were amplified using a modified method according to Klindworth et al. (2013). 100ng of DNA template was amplified with oligos BacV3V4F (CCTACGGGNGGCWGCAG) and BacV3V4R (GACTACHVGGGTATCTAATCC). 250 ng of PCR product was used for library preparation using KAPA HTP Library Preparation Kit for Illumina platforms according to manufacturers protocol, except that the final library amplification was omitted.