Receptacles: untreated
Total RNA was extracted from tissue samples, and the concentration and purity of the extracted RNA were detected by Nanodrop2000. RNA integrity was measured by agarose gel electrophoresis, and the RIN value was measured by Agilent 2100. The magnetic beads with Oligo (dT) were used to perform A-T base pairing with ployA, mRNA was isolated from total RNA, fragmentation buffer was added, and the mRNA was randomly broken into small fragments of about 300bp. Under the action of reverse transcriptase, random hexamers were added, and mRNA was used as a template to reversely synthesize one-strand cDNA, followed by two-strand synthesis to form a stable double-stranded structure. End Repair Mix was added to fill the blunt ends, and then an "A" base was added to the 3 'end for the Y-shaped linker. Then, perform sequencing on the Illumina platform.