R2D2 chr2 77-86Mb HR8_Sample9
Ten individuals from the HR8 selection line were selected for whole-genome sequencing. Five micrograms of high-molecular-weight DNA were used to construct TruSeq Illumina libraries, using 0.5 μg starting material, with 300- to 400- and 400- to 500-bp fragment sizes. Each library was sequenced on one lane of an Illumina HiSeq2000 flowcell, as paired-end reads, with 100-bp read lengths. HR8 sequenced reads were aligned to the mouse genome (mm9) using bowtie 2.2.3 with default options. We removed PCR duplicates and filtered low-quality SNPs using samtools 0.1.19  and Picard. A subset of the bam files for R2D region (77 to 86 Mb on chr2) is submitted.