GSM2462238: mBCC1 stromal RNAseq; Homo sapiens; RNA-Seq
Extraction of high quality RNA (RIN>7 on Agilent RNA pico-chip) was required for RNA sequencing.  Due to the small sample sizes and meticulous LCM, low concentrations of RNA were obtained for some samples. To allow for an impartial comparison, a low input library preparation protocol (SMARTer® Universal low input RNA kit from Clontech®) capable of a range between 200 pg to 10ng was employed for all samples regardless of total yield. Amplification of the low input samples only would have introduced bias when comparing against the standard library preparation. Despite the low input range, sample concentrations were greater than 100ng. Synthesised and adapter ligated copy DNA (cDNA) was then sequenced using 100 bp paired-ended reads on an Illumina® HiSeq2000 averaging 13 million paired end reads per sample by OGT.
GEO Accession
GSM2462238