Illumina MiSeq sequencing of SAMD00217355
Viral RNA was extracted using the Quick Gene RNA cultured cell HS kit (KURABO, Osaka, Japan).  Total RNA was reverse-transcribed using reverse transcriptase (TOYOBO) with an oligonucleotide primer, which is complementary to the nucleotide sequences between position 1 to 20 on the negative-sense RNA of segment 8.  The cDNA derived from segment 8 was amplified by PCR using fusion primers, including the adaptors for the MiSeq sequencing system (Illumina, San Diego, CA): seg8 63 For overhang and seg8 311 Rev overhang corresponding to segment 8 between the nucleotide sequence positions 63 to 85 and 288 to 311, respectively