GSM2461770: BK6_repC; Homo sapiens; RNA-Seq
Total RNA was extracted from isolated BAB and BAN aggregates with Qiagen RNeasy kit according to manufacturer’s recommendation RNA samples of high integrity (RIN value ≥ 8) were then used for RNA-seq library construction. cDNA was generated from 30 ng of extracted RNA with SMARTer Ultra Low Input RNA for Illumina Sequencing (Clontech), followed by RNA-seq library construction with NEBNext DNA Library Prep Master Mix Set for Illumina (NEB). Constructed libraries were multiplex in batches of twelve and 76 bp paired-end sequenced with Illumina HiSeq High Output v3
GEO Accession
GSM2461770