GSM2462106: 10480X4; Macaca fascicularis; RNA-Seq
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Illumina TruSeq Stranded Total RNA Kit with Ribo-Zero Gold HiSeq 101 Cycle Paired-End Sequencing v4  Sequencing libraries (25 pM) were chemically denatured are applied to an Illumina HiSeq v4 paired end flow cell using an Illumina cBot.  Hybridized molecules were clonally amplified and annealed to sequencing primers with reagents from an Illumina HiSeq PE Cluster Kit v4-cBot (PE-401-4001).  Following transfer of the flowcell to an Illumina HiSeq instrument, a 101 cycle paired-end sequence run was performed using HiSeq SBS Kit v4 sequencing reage Sequencing libraries (25 pM) were chemically denatured are applied to an Illumina HiSeq v4 paired end flow cell using an Illumina cBot.  Hybridized molecules were clonally amplified and annealed to sequencing primers with reagents from an Illumina HiSeq PE Cluster Kit v4-cBot (PE-401-4001).  Following transfer of the flowcell to an Illumina HiSeq instrument, a 101 cycle paired-end sequence run was performed using HiSeq SBS Kit v4 sequencing reage
GEO Accession
GSM2462106