GSM3215614: GM12878 bulk SPI1 Rep1; Homo sapiens; ATAC-seq
Cells were sorted by FACS and 5e4 cells were pelleted and resuspended in 50ul of ATAC RSB with 0.1% NP40, 0.1% Tween-20, and 0.01%. After three minutes, 1ml of ATAC RSB with 0.1% Tween-20 was added, tubes were inverted, and nuclei were centrifuged at 500 rcf for 10 min. Supernatant was carefully removed and nuclei were resuspended in 50ul transposition mix (25ul TD buffer, 2.5ul transposase, 16.5ul PBS, 0.5ul 0.1% digitonin, 0.5ul 10% Tween-20, and 5ul water). Transposition was performed for 30 minutes at 37 C with shaking in a thermomixer at 1000 RPM. Reactions were purified with a Zymo DNA Clean & Concentrator 5 kit and library generation was performed as described.
GEO Accession
GSM3215614