GSM4484321: ChATpos4; Mus musculus; RNA-Seq
Total RNA was extracted using the Qiagen RNeasy Plus Micro kit according to the manufacturer's protocol. The RNA quality was assessed with the RNA 6000 Pico assay kit using the Agilent Bioanalyzer. Sequencing-ready cDNA libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep with Sample Purification Beads and NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (New England BioLabs) following the manufacturer's protocol. Bioanalyzer traces were used to confirm library size distribution. The libraries were quantified by qPCR using KAPA Library Quantification kit (Kapa Biosystems).
GEO Accession
GSM4484321