GSM3465639: Fibroblast_3; Homo sapiens; RNA-Seq
Total RNA was extracted and purified using the RNeasy Mini Kit (Qiagen) with an on-column DNase treatment. Poly-A, strand-specific RNA library construction was performed by the Yale Center for Genomic Analysis utilizing the TruSeq Library Prep Kit 2.0 per the manufacturers specifications. . Following first-strand synthesis with random primers, second strand synthesis was performed with dUTP for generating strand-specific sequencing libraries.
GEO Accession
GSM3465639