GSM2373511: H9_amnion_58697; Homo sapiens; RNA-Seq
Samples were collected and prepared for RNA isolation following a protocol commonly used for processing in vivo tissues. In brief, samples were washed with DMEM/F12. Cysts (ca. 5,000 or more per sample) or colonies were then scraped from the culture substrate, before being spun down at 1,000 rpm for 3 min in a conical tube. RNA was isolated from the cell pellet using an RNeasy Micro Kit as suggested by the manufacturer. RNA quality and quantity were determined using a NanoDrop 1000 spectrophotometer, as well as an Agilent 2100 Bioanalyzer. Performed by the University of Michigan DNA Sequencing Core following Illumina guidelines.
GEO Accession
GSM2373511