Sponge
16S V3 and V6 library preparation for the 14 samples was performed at Genotypic Technology’s Genomics facility according to workflow outline above. 4 ng of Nanodrop quantified DNA was used for amplifying 16S Archaea region with degenerate gene-specific primers which have 5’end flanking sequence complementary to Illumina sequence adapter and index primers from the Nextera XT Index kit V2.