GSM3467159: LSRR_1; Lactobacillus salivarius str. Ren; RNA-Seq
Total RNA isolation was carried out using TRIzol reagent (Invitrogen, Carlsbad, CA) as the manufacturer's instructions. Sequencing libraries were generated using NEBNext® Ultra™Directional RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumia) according to the manufacturer's instructions. After cluster generation, the library preparations were sequenced on an Illumina Hiseq platform and paired-end reads were generated.
GEO Accession
GSM3467159