Q117 leaf transcriptome, wild type replicate 1
RNA was isolated from 100 mg of frozen leaf tissue sampled from five random plants 3 h into the light period with the Agilent RNA kit following the manufacturer’s instructions. Four hundred microlitres of extract was purified on multiple columns to avoid column saturation and DNA contamination. The RNA was quantified on an Agilent Technologies 2100 Bioanalyzer following the manufacturer’s instructions. The electropherograms showed that the RNA was not degraded or contaminated with genomic DNA. An Illumina sequencing library for Q117 and 7B4 was prepared from 1 ug of purified mRNA pooled from all replicates following the low-throughput protocol of the Illumina TruSeq sample preparation kit. Library insert sizes were 170 bp. The libraries were indexed and pooled and sequenced by Geneworks (Thebarton, Australia) with the IlluminaGAIIxGenomeAnalyzer using a single lane of paired-end 2 x 90 bp reads (Illumina sequencing reagents v5).