GSM3593577: PDGFRa_I_1; Mus musculus; RNA-Seq
The left lung lobe from tdTomato mice was mechanically separated and a single single cell suspension generated. tdTomato-positive cells were sorted directly into RNA lysis buffer (Qiagen, Venlo, Netherlands) using a FACSAria II cell sorter (BD Biosciences, San Jose, CA, USA). RNA was isolated with the RNeasy micro kit (Qiagen). Library preparation used the SmartSeq2 protocol and sequenced on the Illumina HiSeq 3000 platform
GEO Accession
GSM3593577