GSM4315248: WT3 iPSC-CMs d60 #1; Homo sapiens; RNA-Seq
Total RNA of iPSC-CM cultures was isolated using the SV Total RNA Isolation System (Promega) according to the manufacturer's instructions. RNA-seq libraries were performed using a modified non strand-specific, massively-parallel cDNA sequencing (RNA-Seq) protocol from Illumina starting with 200 ng RNA. The TruSeq RNA Library Preparation Kit v2, Set A (48 samples, 12 indexes; Illumina) was used. Quality and integrity of RNA was determined with the Fragment Analyzer (Agilent) (RIN > 8). The size of final cDNA libraries was determined by using the dsDNA 905 Reagent Kit (Agilent) exhibiting a sizing of 260 bp in average. Libraries were pooled and sequenced on an Illumina HiSeq 4000 (Illumina) generating 50 bp single-end reads (30-40 Mio reads/sample).
GEO Accession
GSM4315248