WGS of BSC cultivated in a potting experiment: BSC grown on soil taken from the Chicken Creek catchment, sampled after ten months of incubation, replicate 3
DNA was extracted using the Genomic DNA from soil NucleoSpin Soil Kit (Macherey-Nagel, DE). DNA was sheared using an E220 Focused-ultrasonicator (Covaris, USA). Library preparation was performed using the NEBNext Ultra DNA Library Prep Kit for Illumina and the NEBNext Multiplex Oligos for Illumina (both New England Biolabs, UK). Sequencing was performed on a MiSeq sequencer using a MiSeq Reagent Kit v3 for 600 cycle (Illumina, USA).