Microbiome of chicken cecum
Metagenomic DNA was isolated using iPrep ChargeSwitch gDNA kit, DNA was then automatically purified using the iPrep gDNA.The 16S rRNA gene sequencing of hypervariable region as per Ion Torrent 16S Metagenomics Kit. Pooled purified amplicons were used to create sequence libraries via the Ion Plus Fragment Library Kit with sample indexing using the Ion Xpress Barcode Adapters 1-96 Kit. Template preparation was performed using the Ion OneTouch 2 System and the Ion S5 OT2 Kit. Sequencing was conducted using the Ion S5 Sequencing reagents on the Ion S5 system using the Ion 530 chip.