COLO320HSR ATAC-seq replicate 1
Briefly, 100-500K cell nuclei were extracted by NPB buffer (5% BSA, 0.2% IGEPAL-CA630, 1 mM DTT, EDTA-free protease, in PBS) at 4C for 10 min. Tagmentation was done in TB buffer (33 mM Tris-acetate pH 7.8, 66 mM K-acetate, 11 mM Mg-acetate, 16% DMF) with Tn5 transposase (Illumina, San Diego, CA), at 37C for 30 min. DNA samples were then extracted and DNA libraries were generated by PCR.