Assembled sequence of Streptococcus mutans: SMB51
Genomic DNA was extracted by TIANamp Bacteria DNA Kit (Tiangen biotech, Beijing, China) according to the instruction of manual. A total amount of 1g DNA per sample was used for the DNA sample preparations. The DNA sample was fragmented by sonication to a size of 350 bp, then DNA fragments were end-polished, A-tailed, and ligated with the full-length adaptor for Illumina sequencing with further PCR amplification. At last, PCR products were purified (AMPure XP system) and libraries were analyzed for size distribution by Agilent 2100 Bioanalyzer and quantified using real-time PCR. The whole genome was sequenced using Illumina NovaSeq 6000 PE150 at Novogene Bioinformatics Technology (Beijing, China). The sequence data were assembled by SOAP denovo software.