GSM2552866: Control3_3; Homo sapiens; RNA-Seq
Adipose tissue was removed surgically, dissected from surrounding connective and vascular tissue on dry ice and frozen in liquid nitrogen, 2-3 frozen aliquots per subject were transferred into 1 mL QIAzol (Qiagen) and homogenized on thawing with a hand-held TissueRuptor (Qiagen) before centrifugation at 13,000g for 3 minutes, and infranatant beneath the fatty layer was mixed with 1 ML 100% ethanol before binding to RNeasy columns and purifying using the RNAeasy kit according to manufacturer's instructions. RNA was concentrated with the Rneasy MinElute Cleanup Kit (Qiagen) and the RNA samples were whole transcriptome-amplified with NuGEN RNA-Seq System V2 and converted to indexed libraries with NuGEN Ovation Rapid DR Multiplex Library System following manufacturer's instructions.
GEO Accession
GSM2552866