DNA-seqs of adapted anaerobic sludge
DNA amplicon libraries were generated using a limited cycle PCR:  initial denaturation at 95C for 3 min, followed by 25 cycles of annealing (95C 30 seconds, 55C 30 seconds, 72C 30seconds) extension at 72C 5 minutes, using a KAPA HiFi HotStart ReadyMix (KK2602). Then llumina sequencing adaptors and dual-index barcodes (Nextera XT index kit v2, FC-131-2001) are added to the amplicon. Libraries are then normalized and pooled prior to sequencing.