GSM2730200: not4D+Not4RRM Replicate 3; Saccharomyces cerevisiae; RNA-Seq
Total RNA was extracted from whole cell cultures using the Qiagen RNA isolation kit. Its quality was verified using the Agilent 2100 Bioanalyzer (Agilent Technologies). 1µg of total RNA was enriched for Poly A RNA using the Dynabeads® mRNA DIRECT™ Micro Purification Kit according to manufacturer’s standard protocol. The resulting enriched RNA samples were then used to prepare barcoded libraries for sequencing using the Ion Total RNAseq V2 kit and the Ion Xpress 1-48 Barcode kit. Libraries were amplified 16 cycles and the quality of the libraries was confirmed on an Agilent Bioanalyzer using a DNA High Sensitivity chip. The libraries were pooled based on concentrations from the High Sensitivity chip, purified on a Pippin Prep gel, quantified by the Agilent Bioanalyzer and sequenced on an Ion Torrent Proton sequencer.
GEO Accession
GSM2730200