GSM4661591: Heart WT rep1; Mus musculus; RNA-Seq
Total RNA was isolated from frozen ventricles of one-day-old mice using Trizol Reagent (15596026, Invitrogen) and was purified and concentrated with RNA Clean and Concentrator-5 (R1013, ZymoResearch). 1.5 μg of total RNA was enriched for polyA+ RNA Illumina TruSeqRNA sample prep according to the manufacturer's standard protocol. Approximately 20 ng of the polyA+ enriched RNA was then used to prepare libraries for sequencing using Kapa stranded RNA seq kit. mRNA was enriched by oligo-dT beads from Illumina and libraries were prepared using Kapa Stranded RNA seq kit according to manufacturer protocol. NEB Next adaptors were used: #77 - > Ad. 6 (GCCAAT) and #209 -> Ad.12 (CTTGTA).
GEO Accession
GSM4661591