GSM3733652: S2 wild-type Rep1; Drosophila melanogaster; ncRNA-Seq
Total RNA extraction with Trizol according to manufacturer's instructions followed by biochemical purification of Argonaute ribonucleoprotein complexes. Libraries were performed using an in-house protocol: ligate 3p linker; size select ligation product; ligate 5p linker; size select ligation product; reverse transcription; qPCR library amplification. 3p and 5p adapters contain 4N at ligation interface (Jayaprakash et al, NAR, 2011)
GEO Accession
GSM3733652