B73 tassel primordium 08
Individual inflorescence primordia were dissected, flash frozen, and disrupted using stainless steel beads. Total RNA was extracted with Trizol, quantified by QuBit, and checked for degradation  on an agarose gel. cDNA libraries were prepared using the NEB ULTRA II RNAseq workflow, including an initial magnectic bead poly-dT selection phase. Libraries were amplified using 7 rounds of PCR and checked by BioAnalyzer followed by qPCR. All libraries within this BioProject were multiplexed using the NEBNext Oligos for Illumina and sequenced in approximately equimolar quantities in one lane.