Collated amplicons from 6 experiments: full-wt, crp-wt, rnap-wt, full-500, full-150, full-0
Synthesized inserts containing partially mutagenized E. coli lac promoters were cloned into a plasmid vector. This resulted in a library of plasmids containing mutant lac promoters driving the expression of GFP. These plasmids were transformed into E. coli. Transformed cells were grown in the presence of IPTG and various concentrations of cAMP, thereby inducing GFP expression. Cells were sorted, using FACS, into 5 or 10 baches based on cellular fluorescence. PCR amplicons (containing batch-specific barcodes) were then generated from the cells in each FACS batch. Finally, barcoded amplicons were collated and sequenced using 454 pyrosequencing. See publication for more details.