GSM4412213: M1-d (rep 1); Mus musculus; RNA-Seq
Total RNA was extracted from tissue after homogenization using the Qiacube (Qiagen) with the RNeasy kit (cat no. 74106). Fresh tissues were disrupted in 3ml Buffer RLT and homogenized by Kinematica AG homogenizer. 1ml of lysate was centrifuged for 5min at 5000 x g., then 600ul of lysate was loaded on the Qiacube for automated extraction. Ethanol precipitation was preformed to all RNA samples to ascent quality of samples. Quality measurements for total RNA were performed using TapeStation (Agilent). The RINe value of most of the samples was above 8.1, except two samples which showed poor quality (RIN value <7.5). Libraries were prepared from the RNA using in-house (Weizmann Institute) protocol. Samples were sequenced on one lane of Illumina HiSeq machine, using the Single-Read 60 protocol. The output was ~24 million reads per sample
GEO Accession
GSM4412213