Miseq sequencing of prokaryotic 16S rDNA during spring bloom in the ECS
To amplify prokaryotic 16S rRNA genes, a prokaryotic universal primer targeting both bacteria and archaea was used (Takahashi et al., 2014). General procedures during PCR amplification, clean up and index PCR for MiSeq sequencing followed the instructions described in the MiSeq manual (Illumina 2013; Illumina, San Diego, CA).