Chinese nong-flavor liquor starter metatranscriptomes
For eukaryotes, poly (A) mRNA was purified using poly-T oligo-attached magnetic beads. For prokaryotes, rRNA was removed first before the subsequent step. The mRNA was fragmented and synthesized into first-strand cDNA using reverse transcriptase and random primers. This step was followed by second-strand cDNA synthesis. Short fragments were purified and connected with sequencing adapters. Then 200 bp cDNAs fragment were  used for further template enrichment by PCR. The validated 200 bp fragment cDNA libraries were submitted to the HiSeqTM 2000 platform for paired-end (PE) RNA sequencing.