GSM4483500: NH4_lim; Candidatus Kuenenia stuttgartiensis; RNA-Seq
Total RNA was isolated using both the TRIzol™ Reagent and the RiboPure™ bacteria kit (ThermoFisher Scientific, Waltham, USA) according to the manufacturer's instructions with the exception that 100-fold higher cell numbers were used, since the recommended cell number resulted in very low to no RNA yields. RNA was eluted in DEPC treated water in volumes between 20-25 μl for the TRIzol™ method and 50-70 μl for the RiboPure™ method, of which 1-2 μl was used to quantify the isolated RNA. The isolated RNA was treated with DNAse 1, as described and provided by the RiboPure™ kit. cDNA libraries were made using TruSeq stranded mRNA Library Prep Kit v2, according to the protocol supplied by the manufacturer (Illumina, San Diego, California U.S.A.).
GEO Accession
GSM4483500