scRNA-seq of Schizosaccharomyces pombe: spore
Single-cell was picked up manually using a Drummond Microdispenser (Drummond Scientific Company) pipette. Total cDNA was prepared and amplified with bead-seq method (Matsunaga et al., 2015). Amplified cDNA was fragmented using the Nextera XT DNA Sample Prep Kit (illumina). Then, sequencing was performed using Illumina HiSeq 2500 with the application of paired end sequencing (2 x 101 nucleotides).