16Sr and ITS1 Metagenome of Phelipanche ramosa seed control
2-step amplicon PCR on 16S V4-V5 region and ITS1 region with primers 16S_515f (GTGCCAGCMGCCGCGGTAA) and 16S_806r (GGACTACVSGGGTATCTAAT) and ITS1_f  (CTTGGTCATTTAGAGGAAGTAA) and ITS2 (GCTGCGTTCTTCATCGATGC), with incorporation of barcodes on 2nd PCR, followed by purification and quantification to build equimolar pools diluted with PhiX phage mix to 12 pM