Small RNA-Seq of MH7A exosomes: non-stimulated Rep2
cDNA libraries were prepared using NEBNext multiplex small RNA library prep set for illumina (Set1) (New England Biolabs, MA, USA) following the manufacture s protocol with modification of library size selection. Amplified cDNA libraries were electrophoresed on 12.5% polyacrylamide gel (E-T 12.5L, ATTO, Tokyo, Japan) and ~140 nucleotide bands (the length of adapter-ligated miRNA constructs) were eluted and precipitated by ethanol.