GSM4484368: spd_1366-2; Streptococcus pneumoniae D39; ncRNA-Seq
RNA was isolated from cells via the Fastprep blue kit (MPBiomedicals) Library was generated by removing DNA by DNase treatment, depleting rRNA with Ribo-Zero rRNA removal kit for gram-positive bacteria.  rRNA depleted samples were then subjected to RNA fragmentation using the Ambion RNA fragmentation kit (AM8740). Fragmented RNA was subjected to RNA 5' polyphosphatase (Epicenter) treatment, which was performed to facilitate the 5' adapter ligation step. Small RNA libraries were generated by Macrogen using TruSeq® small RNA library kit (Illumina).
GEO Accession
GSM4484368