GSM2553013: LKO 2; Mus musculus; RNA-Seq
Total RNA was extracted with Trizol reagent (Invitrogen). Libraries were prepared from 4ng of total RNA with the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (Takara) and subsequently with the Nextera XT DNA Library Preparation Kit (Illumina) according to the manufacturer’s instructions.  These preparation included 10 cycles of preamiplification PCR and 7 cycles of enrichment PCR after tagmentation.
GEO Accession
GSM2553013