Apolygus_lucorum_whole_body
RNA was isolated from adults of Apolygus lucorum using the TRIzol (Invitrogen) reagent by following the company manual. Poly(A) mRNA was isolated from 20  l total RNA using Oligo (dT) magnetic beads and then was broken into short fragments (about 200bp) in the presence of fragmentation buffer at 94  C for 5 min. The mRNA samples were used to construct the cDNA libray with mRNA-Seq assay, which was performed by Novogene (Beijing, China).The libraries were sequenced using HiSeq2500 (Illumina) in paired-end mode, creating reads with a length of 100 bp.