GSM4662902: Input_PCIF1 KO_HIV_rep1; Homo sapiens; OTHER
Total RNA was extracted using TRIzol. About 200 μg RNA were then used to isolate poly(A) RNA using two rounds of oligo25 Dynabeads capture. For one sample, 10 μg poly(A) RNA was de-capped with 10U Cap-Clip enzyme and was used for m6A-MeRIP experiment. Briefly, RNA was fragmented and incubated with 5 μg of anti-m6A antibody (abcam, ab151230) at 4 °C for 2 h. 100 μl protein G magnetic beads were added to pull down the m6A immunoprecipitation complex. After wash for three times, the bound RNA was eluted in 100 μl elution buffer and purified using RNA clean and concentrator-5 kit (Zymo Research). Final libraries amplified and were subjected to paired-end sequencing in the HT sequencing IGM Genomics core, UCSD. MeRIP-seq
GEO Accession
GSM4662902