16s rDNA of wastewater metagenome in an internal aeration microaerobic reactor :sampled at the 140th day
The DNA of sample was extracted by using a Soil DNA Kit (OMEGA, E.Z.N.ATM Mag-Bind Soil DNA Kit). The PCR about 16S rDNA of V3-V4 region was executed with 341F and 805R primers. The DNA products after PCR was detected in the Illumina Miseq 2305bp system (Shanghai Sangon, China).