WGS of Black Bengal Goat: Adult male ear tissue
The DNA was passed quality control and fragmented by acoustic disruption using Covaris S220 and then undergoes end repair, dA-tailing, adapter ligation and purification. The purified DNA was further selected for the right size before PCR amplification for library construction. The preliminary quantification and dilution of the library was performed using Qubit3.0, and then Agilent 2100 was used to determine the insert size and nucleic acid concentration of the resulting library sample. The effective concentration of each sample library in the library mixture was determined by qPCR before sequencing to ensure the accuracy of the sample concentration and the reliability of the sequencing data.