cDNA sequencing of heavy chain antibody transcripts from mus musculus: adult female spleen germinal center B cells
Twelve days post IP immunization with NPCGG,  CD19/GL7 positive germinal center B cells were isolated via FACS. Isolated cells were lysed and cDNA libraries were prepared. IgHV and constant region primers were used to amplify transcripts. PCR products were run on an agarose gel and  extracted for sequencing.