GSM2464129: Thy1.1-YFP+; Mus musculus; RNA-Seq
Lymphocytes were isolated from spleens and lymph nodes of Foxp3 ΔCNS1-Cre-Rosa26-YFP mice and furthur enriched for CD4+ T cells using magnetic beads.  Thy1.1-YFP+ and Thy1.1+YFP+ Treg cells were sorted with purity of typically >97% and RNA was harvested using Trizol reagent. NEBNext® Ultra™ RNA Library Prep Kit for Illumina was used with 3 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM2464129