RNA-Seq of WT or HIF-1a KO Mus musculus: intestinal epithelium cells
WT or epithelium-specific HIF-1a KO adult mice were treated with butyrate and infected with C. difficile. On day 2 post-infection, intestinal epithelial cells were isolated from the colon and the total RNA was extracted. RNA quantification and integrity were performed on a BioAnalyzer. Samples were then sent to BGI for library prep and sequencing.