GSM3523530: PA-3_IEL_ILC3; Mus musculus; RNA-Seq
Small intestine intraepithelial and lamina propria layer's lmphocytes were isolated via enzymatic treatment and percol gradient purification. ILC3s from IEL and LP were analyzed and sorted via FACSAria and RNA was harvested using Qiazol reagent. SMART-Seq v4 Ultra Low Input RNA Kit was used for the construction of sequencing libraries. RNA libraries were prepared using the SMART-Seq v4 Ultra Low Input RNA Kit for amplication and sequencing using standard Illumina protocols
GEO Accession
GSM3523530