GSM4411231: Hela_Drosha_KD; Homo sapiens; RNA-Seq
Total RNA was extracted with TRIzol and Direct-zol RNA Kits (Zymo Research). mRNA was selected using Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) and then fragmented into 150~200 nt fragments by incubation at 94 °C for 8 minutes in fragmentation buffer (NEBNext Ultra II Directional RNA Library Prep Kit, NEB). The fragmented RNA was then used for library construction, following the manufacturer's protocol (NEBNext Ultra II Directional RNA Library Prep Kit, NEB). Libraries were sequenced on Hiseq X10 (Illumina) to produce paired-end 150 bp reads.
GEO Accession
GSM4411231