Oryza rufipogon degradome-seq
(1) Approximately 150 ng of poly (A)+ RNA was used as the input RNA and annealed with biotinylated random primers; (2) 5 adaptor ligation occurred only with those RNAs containing 5-monophosphates; (3) streptavidin capture of RNA fragments occurred through the biotinylated random primers; (4) reverse transcription and PCR were performed; (5) the library was sequenced using the 5 adaptor only, resulting in the sequencing of the first 47 nucleotides of the inserts that represented the 5 ends of the original RNAs.