GSM4160219: Pancreatic islets, 3HF276; Mus musculus; RNA-Seq
Total RNA from islets was extracted using the RNeasy Plus micro kit (Qiagen, Hombrechtikon, Switzerland). Integrity of islet RNA was verified on a Fragment Analyzer (Agilent Technologies, Inc., Santa Clara, CA 95051, USA) and all preparations had a RNA quality number (RQN) between 8.2 and 9.3. RNA-seq libraries were prepared using 250ng of total RNA and the Illumina TruSeq Stranded mRNA reagents (Illumina; San Diego, California, USA). Cluster generation was performed with the resulting libraries using the Illumina TruSeq SR Cluster Kit v4 reagents Sequenced on the Illumina HiSeq 2500 using TruSeq SBS Kit v4 reagents. A sequencing depth of 31 to 46 million reads using 125bp single-end reads was used.
GEO Accession
GSM4160219