GSM2807305: HMECsSox4mindoxplusTGF_S11.merged; Homo sapiens; ChIP-Seq
Crosslink was performed with disuccinimidyl glutarate (DSG) (Thermo Scientifis) for 45 minutes followed by 30 minutes incubation with formaldehyde 1%. The reaction was blocked with glycine 100 mM. Shearing was performed using Covaris S2 (Covaris, Woburn, MA) for 8 min at maximum intensity. The sonicated chromatin was incubated O/N at 4°C in presence of 15µl of anti-SOX4 (CS-129-100, Diagenode) or 2µg of anti-SMAD3 (ab28379, abcam) coupled to A/G sepharose beads (Santa Cruz Biotechnology). Kapa Hyper Prep Kit (Kapa Biosystems, Wilmington, MA) was used for End-repair, A-tailing and ligation of sequence adaptors. Samples were amplified by PCR and the libraries were size-selected in the 200-500 bp range.
GEO Accession
GSM2807305