RNA-Seq of Phytophthora cactorum: liquid culture (isolate 440)
RNA was extracted from 5-day old liquid culture (PG1) with Tripure isolation reagent (Roche Life Science) and RNeasy Mini Kit (Qiagen). On-column DNase Digestion was made with RNase-Free DNase Set (Qiagen). The libraries were constructed with Illumina Truseq RNA-seq Sample Prep Kit, and the sequencing was made using Illumina HiSeq2000.