WGS of  Lactobacillus murinus, strain NM26_J9: murine gut isolate
Sequencing  performed by Dr. Brian Yu at the Genomics Platform of the Chan Zuckerberg  Biohub. Nextera XT kits were used to  make the libraries with CZ Biohubs custom designed adapters - 12 bp index  sequences on each side of the library and are dual unique so that each  sequence only appears once. The libraries were made in 384 well plates in 4  uL of total volume. Genomic DNA was diluted to 0.18 ng/uL and 0.4 uL was  added to the library prep reaction using Mosquito HTS from TTP. 0.5 uL of the  library was pooled for sequencing. Sequencing was performed on a NovaSeq S4  flow cell with the run configuration 140 (12) (12) 140, so thats 2x140 bps  with dual 12 bp index sequences.