GSM1232750: SigX_empty_vector_sample2; Pseudomonas aeruginosa UCBPP-PA14; RNA-Seq
Using Rneasy kit (Qiagen) plus Qiashredder columns. Customized protocol (Dötsch et al., PloS ONE, 2012). Briefly, rRNA depletion using MICROBExpress kit with the Pseudomonas module (Ambion) and fragmentation by sonication using Covaris Adaptive Acoustics device (Covaris). Upon ligation of barcoded adapters, reverse transcription with SuperScript II kit (Invitrogen) followed. Amplification was done by PCR using Illumina platform compatible primers. Established cDNA library was normalized by double strand specific nuclease treatment (Evrogen) according DSN Normalization Sample Preparation Guide from Illumina.
GEO Accession
GSM1232750