MiSeq of fungi on green and non-green materials
The sequences were clustered into OTUs defined at the level of 97% sequence identity using the UPARSE algorithm, after going through de-noising and chimer checking (Edgar, 2013).  The denoising was done to remove short sequences, singleton sequences, and noisy reads. Next, the USEARCH global alignment algorithm or the RDP Classifier against a proprietary database (Research and Testing Laboratory) of high-quality sequences derived from the GenBank as they existed on December 2014 was used to query the centroid sequence from each cluster.