GSM4483344: SN_Donor6OTC; Homo sapiens; RNA-Seq
Nuclei isolation by lysing cryosections of frozen brain tissue in a sucrose buffer with Triton. Then purifying the nuclei by dense sucrose gradient ultracentrifugation. Nuclei are FACS sorted on DAPI positive expression, NeuN and Olig2. 10x Genomics Single Cell v3 kit or 3' Quantseq FWD (Lexogen)
GEO Accession
GSM4483344