GSM3733367: m6A_IP2; Homo sapiens; RIP-Seq
total RNA was extracted using Direct-zol (Zymo) from embryos homogenized in TRIzol. mRNA was further purified using Dynabeads mRNA DIRECT Kit. For IP samples, mRNA was sonicated and subjected to IP using m6A antibody (Synaptic Systems, 202003). Libraries were constructed using TruSeq Stranded mRNA Library Prep Kit (Illumina).
GEO Accession
GSM3733367