GSM2043765: 240614 0518-370neg ILC3; Homo sapiens; RNA-Seq
Due to low cell counts, lysates were treated as single cells in an adapted SMART-Seq II protocol (Trombetta et al., Current Protocols in Molecular Biology, 2014, 107:II:4:22). Briefly, mRNA was extracted from lystates using SPRI beads and then reverse transcribed and amplified using a poly-adenylated RT primer and template switching SMART oligo. Library construction followed from the protocol above (Trombetta et al.,).
GEO Accession
GSM2043765