RAD-seq of Lysiphlebus fabarum
Samples kept in ethanol at -20 degree C until DNA extraction using a Qiagen DNeasy Blood and Tissue Kit, following manufacturer instructions. Individuals were sequenced in 6 separate libraries, following the protocol from Parchman et al, 2012, with the enzymes EcoRI and MseI, and size selection on agarose gel (200-450bp). Samples were multiplexed in each library following the TruSeq multiplexing design, and libraries were pooled by pairs on the same Illumina lane using adapters iA06 or iA12. Single-end sequencing was performed using Illumina Hiseq 2500.