GSM3527341: RNA-seq of ConvD [Sample 4-7]; Mus musculus; RNA-Seq
Cells enriched by flow cytometry were directly collected in RNA extraction buffer (RLT-buffer supplemented with beta-Mercaptoethanol) Quantity of total RNA was assessed with the Qubit 2.0 and quality was checked using a RNA 6000 Pico chip on Agilent's bioanalyzer. Barcoded mRNA seq libraries were prepared from 22 ng (Sample 1 and 2) or 1ng (Sample 3 and 4) total RNA using a NEBNext® Poly(A) mRNA Magnetic Isolation Module and NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (Sample 1 and 2) or NEBnext Low Input kit for Illumina (Sample 3 and 4) according to the manual. All quality controls were done using Invitrogen's Qubit HS assay and fragment size was determined on Agilent's 2100 Bioanalyzer HS DNA assay.
GEO Accession
GSM3527341