GSM2883182: PlnR9C/+; Mus musculus; RNA-Seq
A standard Langendorf preperation was performed on the hearts of two 18 week old male mice, PlnR9C/+ or Pln+/+. The left ventricle was harvested from the hearts and non-cardiomyocytes were seperated from cardiomyocytes using gravity seperation. Cells were rinsed 3 times in cold PBS, counted using a hemacytometer, and loaded onto a Genomics 10x chip following the manufacturer's protocol to capture single cells. RNA libraries were prepared for sequencing using the standard protocol for the Genomics 10x Chromium Single-Cell 3' v2 Reagent Kits.
GEO Accession
GSM2883182