GSM4159164: primary neuroendocrine tumor; Homo sapiens; RNA-Seq
Both primary neuroendocrine tumor and metastatic liver lesion were processed simulatneously, to avoid batch effects. Single cell suspensions were prepared via both mechanical and enzymatic digestion protocols (collagenase I, DNAseI and TrpLE) .Viable cell count was determined using trypan blue exclusion and a total of 10,000 cells per sample were loaded onto the 10X microfluidic chip.  mRNA from individual cells were captured and tagged with barcodes using 10X-Genomics microfluidic platform. cDNA was then prepared in the encapsulated gel bead emulsion and the emsulion was broken for further library preparation. Barcoded cDNA libraries were prepared according to manufacturer's instructions, 10X Genomics.
GEO Accession
GSM4159164