GSM2885086: miRNA T. muris exosomes rep 1; Trichuris muris; miRNA-Seq
Two different biological replicates of EVs obtained from two different batches of worms were used. ODG fractions with a density between 1.06 and 1.08 (fractions containing pure EV samples after TRPS analysis) were pooled and excess Optiprep® solution was removed by buffer exchanging. miRNA were extracted using the mirVana miRNA Isolation Kit (ThermoFischer) according to the manufacturer's instructions. miRNA was prepared for sequencing using Illumina TruSeq Small RNA-seq library preparation kit according to the manufacturer's instructions.
GEO Accession
GSM2885086