GSM4483696: S33; Homo sapiens; RNA-Seq
Chromium Single Cell V(D)J Reagent Kits (CG000086 Rev G) Cells were lysed within GEMs generated by the 10X platform to yield cell-specific transcriptomic, cell surface protein and TCR information. Library preparation was performed as per the manufacturer instructions which differed slightly for the two batch runs (details above). Essentially, mRNA and cell-surface proteins were tagged with a unique cell-specific oligonucleotide (UMI). Size-based separation and targeted amplification were employed to define 3 different library types : Gene expression, TCR and cell surface protein (containing both cell surface protein and hashtag information). Sequencing was performed on either a HiSeq4000 (S21-S34) or Novaseq6000 (Pooled Sample 1-4) platform. 10x RNA-Seq, 10x feature barcoding, 10x VDJ
GEO Accession
GSM4483696