DNA-seq mate-pair of S. pimpinellifolium cultivar LA0480; Insert Size 2kb
DNA was extracted from whole flowers of a single soil-grown plant LA0480-ref using the Qiagen DNeasy Plant Mini Kit (Qiagen, Germany). Two 101 bp paired-end (PE) short-read libraries (139 and 332 bp mean insert length) and five 100 bp mate-pair libraries (2, 6, 8, 10, and >10 kb insert length) were prepared using the NEBNext Ultra DNA Library Prep Kit and the Nextera Mate-pair Library Kit, respectively (New England Biolabs, United Kingdom).