RNA-Seq of head kidney from Aphanomyces invadans infected Labeo rohita at 6day post-infection, biological replicate 1
The cDNA sequencing library from total RNA has been prepared following the protocol and using the consumables given with NEBNext ultra directional RNA library prep kit (New England BioLabs, MA, USA). The library was quantified using Qubit fluorometer and its fragment size distribution was analyzed on Agilent 2200 Tape system. Sequencing of library was performed on Illumina HiSeq2500 with 2x100 bp paired-end chemistry to generate sequence reads.