RNA-Seq of Ammopiptanthus nanus: leaves of unstressed seedlings
To investigate the expression change of mRNAs and lncRNAs in Ammopiptanthus nanus, seedlings were devided into 6 group, i.e., CK_1, CK_2,CK_3, CT_1,CT_2,and CT_3. Total RNAs were extracted using TRIzol reagent (Invitrogen, Burlington, Canada). The quality and purity of the RNA samples were examined using the RNA 6000 Nano Chip Kit and Bioanalyzer 2100 (Agilent Technologies, Santa Clara, USA) (RIN number > 7.0).Strand-specific libraires were contructed and sequenced at an Illumina Hiseq2500 platform.