GSM2461677: UBR7-shRNA-H3K9me3_ChIP-seq; Homo sapiens; ChIP-Seq
Histone-DNA complexes were isolated using antibodies described from Control and UBR7-shRNA sonicated nuclei. Libraries for Illumina sequencing were generated following the New England BioLabs NEBNext Ultra DNA Library Prep Kit protocol. A total of 10x cycles were used during PCR amplification for the generation of all ChIP-seq libraries. Amplified ChIP DNA was purified using double-sided AMPure XP to retain fragments ~200 to 500bp and quantified using the Qubit 2000 and Bioanalyzer 1000 before multiplexing.
GEO Accession
GSM2461677