Illumina HiSeq 2000 sequencing; RNAseq of primary human gallbladder cells, reprogrammed human gallbladder cells, and human pancreatic beta cells
RNAseq of primary human gallbladder cells, reprogrammed human gallbladder cells, and human pancreatic beta cells
In vitro culture protocol for human gallbladder cells. Dissociated cells from human gallbladder and cystic duct were cultured in rat mammary feeder culture using a modified culture medium as published in PMID 23562832. Isolation of RNA from gallbladder cells and beta cells using Qiagen RNeasy protocol TruSeq RNA sample library preparation v2 as recommended by the manufacturer Illumina
Experimental Factor: epithelial cell of gall bladder
cell type
Experimental Factor: no enrichment
phenotype