WGBS of macaca mulatta: old male peripheral blood
DNA fragments were treated twice with bisulfite using EZ DNA Methylation-Gold TM Kit (Zymo Research). And the resulting single-strand DNA fragments were PCR amplificated using KAPA HiFi HotStart Uracil + ReadyMix (2X). Library concentration was quantified by Qubit 2.0 Flurometer (Life Technologies, CA, USA) and quantitative PCR, and the insert size was checked on Agilent Bioanalyzer 2100 system.