RNA-Seq of human metagenome: necrotizing soft tissue infection site tissue biopsy
Tissue from necrotizing soft tissue infection site biopsies were obtained intraoperatively and stabilized in RNAlater (Thermo Fisher Scientific). Tissue biopsies were transferred into TRIzol (Invitrogen) reagent containing vanadyl ribonucleoside complex. Tissues were homogenized and cells were lysed with a beat beater. RNA extraction was based on the TRIzol method. Total RNA DNase treated and rRNA depleted. RNA libraries were prepared using the ScriptSeq v2 RNA-Seq Library Preparation kit.