16S rRNA sequencing of Homo sapiens: gut metagenome
Microbial DNA was isolated with PowerSoil DNA isolation kit (MO BIO Laboratories, Inc., Carlsbad, CA) from samples stored at ?80 C. Amplification and sequencing of V4 hypervariable region of the 16 S rRNA gene were performed using the validated, region-specific bacterial primers 515F and 806R according to a previously described method.