RNA-Seq of rattus norvegicus: adult male heart
1 ug total RNA was amplified and transcribed into ds cDNA and constructed RNA library by Quant-iT PicoGreen dsDNA Assay Kit (Life Technologies, Carlsbad, CA, USA) and Qubit (Invitrogen). After clustering generation with cBot (Illumina, San Diego, California, USA), the arrays were scanned by the Hiseq2500 SBS (Illumina) and the raw data was subsequently processed