GSM2112892: SLOT (MPRA) run 6 140619; Mus musculus; DNase-Hypersensitivity
ChIP was performed according to the “Mammalian ChIP-on-chip” protocol (Agilent) using a polyclonal antibody against Nrf1 antibody (ab34682, Abcam) and Protein G Dynabeads (Life Technologies). 10-100 million cells were used for each experiment. qPCR using positive and negative control primers was performed to ensure ChIP enrichment. Library preparation and Illumina HiSeq were performed by the MIT BioMicroCenter. DNase-seq was performed as described previously (Sherwood et al., 2014 PMID 24441470). 10-100 million cells were digested with 60-100 units of DNase I (Promega) per 107 nuclei. 50-125 bp hypersensitive DNA was collected using E-Gel SizeSelect Agarose 2% gels (Life Technologies). Library preparation and Illumina HiSeq were performed by the MIT BioMicroCenter.
GEO Accession
GSM2112892