GSM3225981: rec-wt-0hTNF-Repl1: RNA-Seq HeLa wt 0h TNF Repl1; Homo sapiens; RNA-Seq
For RNA-Seq cells were harvested and total RNA was isolated using the NucleoSpin RNA Kit (Macherey-Nagel).  For ChIP-Seq samples were crosslinked using Formaldehyde, Sonicated and chromatin was extracted using the p65 Antibody. DNA was purified NucleoSpin Gel and PCR Clean UP Kit with buffer NTB (Macherey-Nagel). RNA-Seq libraries were prepared from total RNA using the TruSeq Stranded total RNA LT kit (Illumina). ChIP-Seq libraries were prepared from purified ChIP DNA with the Microplex Library Preparation Kit (Diagenode)
GEO Accession
GSM3225981