ChIP-Seq in human MCF7 and HEPG2 cells
ChIP-Seq in human MCF7 and HEPG2 cells
MCF7 human breast cancer cells were grown as previously described (Neve et al. 2006). Unless otherwise stated, during all experiments oestrogen was added at a final concentration of 100 nM for 45 minutes (ChIP) or 24 hours (cell cycle analysis). HepG2 cells were grown in DMEM supplemented with 10% FBS. ChIP experiments were performed with well-characterized antibodies against CTCF (Millipore, 07-729), STAG1 (abcam, ab4457), RAD21 (abcam, ab992), ERa (santa cruz, sc-543), CEBPa (santa cruz, sc-9314) and MED12 (abcam, ab70842). ChIP-seq experiments were performed as recently described (Schmidt et al. 2009)
Experimental Factor: IMMUNOPRECIPITATE
CTCF
Experimental Factor: CELL_LINE
MCF7
Experimental Factor: COMPOUND
oestrogen
Experimental Factor: KNOCK_DOWN
CTCF