Transcriptional responses to hyperplastic MRL signalling in Drosophila
After microdissection of wings imaginal disks, RNA extractions were performed using the Ambion RNAqueous-Micro Kit (Invitrogen). RNA concentrations were measured at 260 nm with NanoDrop1000 spectrophotometer (Thermofisher) and RNA integrity was assessed with Agilent 2100 Bioanalyser. mRNA was enriched from total RNA samples, using the Dynabeads® mRNA Purification Kit for mRNA Purification from Total RNA Preps (Invitrogen). The libraries were prepared according to theScriptSeq v2 RNA-Seq Library Preparation Kit protocol (Epicentre).