GSM4159885: cox4d _t_0; Saccharomyces cerevisiae S288C; RNA-Seq
total RNA was extracted using a standard yeast RNA extraction protocol (using hot, acidic phenol); briefly, cells are resuspended in a lysis buffer and acidic phenol, then incubated at 65C for 30 minutes to dissolve the proteins/membranes and release the RNA.  Next RNA is purified using alcohol precipitation. RNA-seq libraries were prepared using purified RNA isolated as described above. RNA quality and concentration were assayed using a Fragment Analyzer instrument. RNA-seq libraries were prepared using the TruSeq Stranded Total RNA kit paired with the Ribo-Zero rRNA removal kit (Illumina, San Diego, CA). Libraries were sequenced on an Illumina HiSeq 4000 instrument.
GEO Accession
GSM4159885