GSM2043898: FVB/N_WT_24w_#1; Mus musculus; MBD-Seq
genomic DNA was isolated with AllPrep DNA&RNA kit (Qiagen, Germany) and fragmented to ≈ 150bp with Covaris S2 sonicator. Enrichment for highly methylated fragments was performed with Methyl CpG immunoprecipitation (MCIp) according to Sonnet et al, (Methods Mol Biol, 2013) with 5µg of genomic DNA as input and 60µg of MBD2 fusion protein 10ng MCIP-enriched DNA was used as input for library preparation according to the manufacturer's protocol  for the Illumina ChIP Sample Prep Kit with minor modifications.
GEO Accession
GSM2043898