GSM2462753: ES2-Liquiritigenin-1; Homo sapiens; RNA-Seq
ES2 cells were treated with either vehicle or liquiritigenin for 24 h, and total RNA was isolated using RNAesy mini kit (Qiagen) according to the manufacturer’s instructions. The purity of isolated RNA was determined using Agilent 2100 BioAnalyzer. Illumina TruSeq RNA Sample preparation was performed following manufacturer's protocol, and the samples were run on an Illumina HiSeq 2000 in duplicates. Total RNA samples about 1-2 mg were used for sequencing library preparation according to Illumina TruSeq Total RNA Sample Preparation Guide (Illumina Cat. #: RS-122-2201). Each library was bar-coded and then pooled for cluster generation and sequencing run with 100bp single-end (SE) sequencing protocol.
GEO Accession
GSM2462753