GSM2888358: input DNA_rep2; Sulfolobus acidocaldarius DSM 639; ChIP-Seq
cell pellets fragmented by sonication. Then the cleared lysate were mixed with Saci_1107 specific antibody, pre-immune serum, respectively. Immunoprecipitated DNA is extracted by IPure kit (Diagenode) according to the manufacturer's instructions. Sequencing libraries were prepared using the ThruPLEX®-FD Prep Kit (Rubicon Genomics) under instructions from the manufacturer. Clustered using onboard clustering and sequenced on MiSeq (MCS 2.4.1.3/RTA 1.18.54) with a 1x51 setup in High Output mode.
GEO Accession
GSM2888358