Human Yap65 variants Round III library CA0002.SAM2.EXP1
Phage library DNA was isolated by phenol/chloroform extraction and ethanol precipitation. 50 ng of phage DNA was amplified using the Phusion HF system and the DF-97_PCR_Long primers (Supplementary Table 3) for 15 cycles according to the manufacturers instructions (New England Biolabs, Ipswitch, MA). The product was isolated using the Qiaquick PCR purification kit (Qiagen, Germantown, MD) and purified using the Ampure reagent (Beckman-Coulter, Danvers, MA). Concentrations were determined using the Quant-IT dsDNA HS kit according to the manufacturers instructions (Invitrogen, Carlsbad, CA). 0.36 fmol total DNA from each library was loaded into its own lane and sequenced using a Genome Analyzer IIx (Illumina, San Diego, CA) with the DF-97_SEQ_Short primers.