RRBS of Strongylocentrotus purpuratus: 6000 pooled prism; NU (maternal low pCO2, high temp, offspring high pCO2, low temp), replicate 3
Adult purple sea urchins, Strongylocentrotus purpuratus, were conditioned to either simulated upwelling (low temperature, high pCO2) or non-upwelling (average temperature, low pCO2) laboratory conditions during gametogenesis. The offspring of both female adult groups were raised under the same of reciprocal treatment in three replicate cultures. RRBS and gene expression libraries were generated from pooled larvae from each culture.