GSM2807156: ZT21 LDR; Emberiza melanocephala; RNA-Seq
The total RNA was extracted from liver using Trizol Plus RNA Purification Kit and included DNase treatment with Ambion RNase-Free DNase Set and column cleanup with Purlink RNA Mini Kit (Invitrogen), as per manufacturers’ recommended protocol. Ribogreen assay (Invitrogen, xxxx) determined the sample’s RNA concentration and RNA Integrity Number (RIN; Schroeder: 2006ga) on the Agilent 2100 Bioanalyzer. 1μg of total RNA was used to generate poly-A selected mRNAs, which were isolated using oligo-dT attached magnetic beads, and the complimentary DNA (cDNA, 150 – 500 bp with mean size of 250 bp) libraries were generated with TruSeq RNA Sample Preparation Kit v2 (Illumina, San Diego, CA, USA). The quantified (Quant-iT PicoGreen assay; Life Technologies) and validated (Agilent 2200 TapeStation system) libraries were sequenced as 100-base single-end reads on the Illumina HiSeq 2500 at the Salk Institute (San Diego, La Jolla, CA). TruSeq mRNA Sample preparation kit, v2, Illumina
GEO Accession
GSM2807156