Genome-wide comparative analysis of DNA methylation between cytoplasmic male sterility line H276A and its maintainer line H276B in Gossypium barbadense.L
1 g genomic DNA was randomly fragmented to approximately 250 bp by Bioruptor (Diagenode, Belgium). Then, the fragmented DNAs were combined with end repair mix and incubated at 20  for 30 min. The repaired DNAs were ligated to the methylated adapters and the Adenylate 3` Ends DNA and purified with MiniElute PCR Purification Kit (QIAGEN, Valencia, CA). The purified products were exposed to bisulfite treatment and purification with Methylation Gold Kit (Zymo Research, CA, USA). After that, the DNA library was constructed by PCR amplification. The library was qualified by the Agilent Technologies 2100 bioanalyzer (Agilent, CA, USA) and real-time quantitative PCR (TaqMan Probe). The qualified libraries were sequenced pair end on Illumina HiSeq X platform (BGI, Shenzhen, China).