transcriptome of Luffa cylindrica: 2D-2
Ribosomal RNA was removed from total RNA for obtaining mRNA of the samples. Then fragmentation buffer was added to break RNA into small fragments which used for further synthesis of the first strand cDNA. Buffer, dNTPs (dUTP, dATP, dGTP, and dCTP), and DNA polymerase I were subsequently utilized to synthetic the second chain. Obtained double-stranded cDNA was purified by AMPure XP beads. After terminal repairing, A tail adding, indexing adapters ligation and fragment size selection, PCR amplification was conducted to enrich the short fragments for constructing the final cDNA library. The cDNA library was further quantified and its insert size was detected to ensure the quality can achieve standard for sequencing. Sequencing was performed on the Illumina HiSeq Platform.