GSM2807359: T11_neutrophil_2; Mus musculus; RNA-Seq
To obtain tumor-associated neutrophils and macrophages, tumors were dissociated into single cell suspension according to the protocol of Tumor Dissociation Kit, mouse (Miltenyi Biotec). Cell sorting was performed using Aria1 cell sorter (BD Bioscience) based on the following gating strategy: DAPI- CD45+ CD11b+ Ly6G+ Ly6C med-low for neutrophils, DAPI- CD45+ CD11b+ Ly6G- Ly6C- F4/80+ for macrophages. Myeloid cells were sorted directly into TRIzol LS and were frozen at -80C till further processing. RNA was extracted using Direct-zol RNA microprep kit (Zymo). RNA libraries were prepared for sequencing using protocol reported in Sheng et al, Nature Methods 14, 267–270 (2017)
GEO Accession
GSM2807359