GSM3526647: Microglia cells from Trem2 +/- animal 943 after 12 weeks on control diet; Mus musculus; RNA-Seq
Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer.
GEO Accession
GSM3526647