GSM2552885: 6059055; Homo sapiens; RNA-Seq
Poly(A)+ RNA was prepared from whole blood in PAXgene Blood RNA tubes that had been stored at -80 oC. Following the manufacturer’s protocols, RNA was extracted using the PAXgene Blood Kit (PreAnalytiX) and quantified by spectrophotometry. A total of 1.2 µg of total RNA per sample was processed using the GLOBINclear Human kit (Ambion) in order to remove globin mRNA. The quantity and quality of the RNA was analyzed on a Bioanalyzer Eukaryote Total RNA Nano chip. Sequencing libraries were prepared using the TruSeq Stranded mRNA Library Prep Kit (Illumina).
GEO Accession
GSM2552885