GSM2559634: 22Rv1-RED-6h; Homo sapiens; RNA-Seq
Total RNA was isolated using the 'RNeasy Mini' kit (Qiagen) following the manufacturer's instructions. Quality of extracted RNA was evaluated using a 2200 TapeStation system (Agilent). Samples with a RIN score ≥ 7 were selected for making libraries. RNA sequencing (RNA-seq) libraries were built using the 'Stranded mRNA-Seq' kit for Illumina plateforms (KAPA) with OligodT magnetic beads to enrich the samples for informative mRNA species. Libraries were run on a 2200 TapeStation system (Agilent) to confirm fragment size. Libraries were then quantified by qPCR using the 'Library Quantification' kit (KAPA). Sequencing was done on a HiSeq 2000 sequencing system (Illumina) in a 100bp, paired-end run, with three samples per lane
GEO Accession
GSM2559634