GSM2559704: R_HET; Mus musculus; RNA-Seq
Two embryo heads from each genotype (wt, het, mut) at E8.5 were dissected in sterile DEPC treated 1X PBS, lysed in 350 µl of buffer RLT (RNeasy Miniprep Kit-Qiagen) using a tissue lyser (TissueLyser II-Qiagen), and total RNA purified from sample lysates using the RNeasy Miniprep Kit (Qiagen) per the manufacturers’ protocol. PolyA+ RNA was isolated from total RNA by oligo-dT selection and stranded paired-end libraries were prepared and sequenced on an Illumina HiSeq 2500 platform at The Centre for Applied Genomics at SickKids. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM2559704