Illumina HiSeq 2000 sequencing; miRNA profiling by high-throughput sequencing of pancreatic adenocarcinoma (PDAC) xenografts
miRNA profiling by high-throughput sequencing of pancreatic adenocarcinoma (PDAC) xenografts
"two tumor sampling methods from patients with PDAC: 1- surgery for patients with tumor size < 2cm, and  2- Endoscopic Ultrasound-Guided Fine-Needle Aspiration (EUS-FNA) for patients with tumor size > 2cm. this information is given by the \""Resectable\"" variable." "For operated patients (with surgery), tumor tissues were xenografted directly. However, biopsy tissues were grown in culture for 1 or 2 days, before transplanted to the nude mouse. The final xenograft samples were collected from mouse when tumors reached a size of 1 cm. The \""Differentiation\"", \""Fibrosis\"", \""Inflammation\"" information are estimated on the collected xenograft tissues." miRNA-Seq libraries are performed from at least 1ug of extracted total RNA with a RIN greater than 7. Before starting, total RNAs are purified with Trizol-GT & miRNeasy kit wich allows the selection of the small RNA fraction less than 100b.
Experimental Factor: basal
MultiOmicsClassification