GSM4661874: non_malignant_hypertension_154; Rattus norvegicus; RNA-Seq
After organ weighing, kidneys were decapsulated. Both poles of each kidney were immediately snap frozen on liquid nitrogen for RNA extraction. RNA was isolated from tissue samples after homogenization with a disperser (T10 basic Ultra-Turrax; IKA, Staufen, Germany) using the RNeasy Fibrous Tissue Midi Kit (Qiagen, Hilden, Germany) Sequencing libraries were generated from 0.5 ug high quality RNA using the TruSeq Stranded mRNA Kit (Illumina, San Diego, U.S.A.) according to manufacturer's instructions. Libraries were sequenced on a HiSeq 2500 platform (Illumina, San Diego, U.S.A.) as 101 bp single-end reads to a depth of at least 25 million reads.
GEO Accession
GSM4661874