GSM2885088: mRNA T. muris exosomes rep 1; Trichuris muris; RNA-Seq
EVs obtained from one batch of worms were used. ODG fractions with a density between 1.06 and 1.08 (fractions containing pure EV samples after TRPS analysis) were pooled and excess Optiprep® solution was removed by buffer exchanging. Total RNA was extracted using the mirVana miRNA Isolation Kit (ThermoFischer) according to the manufacturer's instructions. mRNA was prepared for sequencing using Illumina TruSeq stranded mRNA-seq library preparation kit according to the manufacturer's instructions.
GEO Accession
GSM2885088