GSM3467234: VA_0h_rep3; Vitis amurensis; ATAC-seq
Harvest and count cells;Centrifuge 50,000 cells 5 min at 500 × g, 4°C;Wash cells once with 50 μl of cold PBS buffer. Centrifuge 5 min at 500 × g, 4°C;Gently pipet up and down to resuspend the cell pellet in 50 μl of cold lysis buffer. Centrifuge immediately for 10 min at 500 × g, 4°C;Discard the supernatant, and immediately continue to transposition reaction;Transposition reaction using transposition reaction mix;Resuspend nuclei pellet (from step 5) in the transposition reaction mix;Incubate the transposition reaction at 37°C for 30 min;Immediately following transposition, purify using a Qiagen MinElute PCR Purification Kit;Elute transposed DNA in 10 μl elution buffer (Buffer EB from the MinElute kit consisting of 10 mM Tris·Cl, pH 8);PCR amplification. ATAC-seq libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM3467234