Whole-genome sequencing of Gymnopus dryophilus
DNA extractions were performed on 1-2 g of fungal biomass after homogenization under liquid N in a sterilized mortar and pestle using a MoBio PowerMax Soil DNA Isolation Kit following the factory protocol with modifications as described by the 1000 Fungal Genomes Project modified protocol (http://1000.fungalgenomes.org/home/wp-content/uploads/2012/03/MObio_Powersoil_genomicDNAProtocol-.pdf). Genomic DNA was sheared into 450-650 bp fragments (mean insert size estimated at 455 bp +/- 110 bp standard deviation) and subjected to sequencing on a single lane of an Illumina HiSeq 2 x 250 bp paired-end sequencing reaction.