GSM3524842: control OPC rep3, input; Mus musculus; OTHER
Lysis buffer (Bio-Rad Aurum total RNA Mini Kit , Cat# 732-6820) was added to culture dishes, cells were then removed by cell scrapers, and flash frozen on dry ice. RNA was extracted using the Bio-Rad Aurum total RNA Mini Kit (Cat# 732-6820) . The equivalent amount of input and m6A-imunoprecipitated RNA were prepared for library generation using the SMART-seq protocol as described (Picelli et al., 2014)
GEO Accession
GSM3524842