Transcriptional identification of miRNAs related to fruit maturation in blueberry（Vaccinium corymbosum-BF-miRNAs）
5-year-old blueberry trees (Vaccinium corymbosum “Northland”) grown at the experimental station in Jilin University (Changchun, Jilin Province, China) were used. Fruit sample was collected from multiple plants at blue fruit developmental stages, which correspond to the stage S7/8 as described previously (Zifkin et al. 2012), respectively. Total RNAs were extracted from all the fruit samples using RNAprep Pure Plant Kit (Tiangen Inc, China) according to the manufacturer’s instructions. One small RNA (sRNA) library was constructed from fruits at blue stage (BF). Finally, sRNA were profiled by single-end sequencing (36 bp) on an Illumina Hiseq 2500.