GSM3070915: WAGO-4-associated small RNAs from F1 after oma-1 dsRNA P0; Caenorhabditis elegans; miRNA-Seq
Small RNAs were cloned and deep sequenced using an Illumina platform, according to the manufacturer's instructions, by the Beijing Genomics Institute (BGI Shenzhen). sRNAs ranging from 17 to 30 nt were gel-purified and ligated to a 3' adaptor (5'-pUCGUAUGCCGUCUUCUGCUUGidT-3'; p, phosphate; idT, inverted deoxythymidine) and a 5' adaptor (5'-GUUCAGAGUUCUACAGUCCGACGAUC-3'). The ligation products were gel-purified, reverse transcribed, and amplified using Illumina's sRNA primer set (5'-CAAGCAGAAGACG GCATACGA-3'; 5'-AATGATACGGCGACCACCGA-3'). The samples were sequenced using an Illumina Hiseq platform.
GEO Accession
GSM3070915