GSM2883846: mES H3K27me3 ChIP-seq; Mus musculus; ChIP-Seq
Cultured cells were harvested and formaldehyde fixed (1%) and processed for ChIP experiments. ChIPs were performed according to the Millipore ChIP protocol, as described previously (De Gobbi et al., 2007). For ChIP-Seq experiments, Embryonic Stem cells were fixed with 1% formaldehyde for 10 minutes at RT and chromatin was sonicated to a size <500 bp. Immunoprecipitations were performed, after an overnight incubation with the appropriate antibody, followed by protein A agarose bead incubation (Millipore). Agarose beads are then washed and eluted and immunoprecipitated DNA  purified by phenol and chloroform extraction followed by ethanol precipitation. The library was constructed using Illumina protocol. The library was amplified prior to size selection (150-200bp).  Model- GAIIx Chemistry-SBS v4 cBot single generation kit with v4 flow cell SCS 2.8 RTA 1.8 Recipe v7.4
GEO Accession
GSM2883846