EdUseq-HU/release of U2OS cells overexpressing cyclin E for 2-3 days: 6 h HU arrest and release for 150 min.
U2OS cells overexpressing cyclin E were synchronized by mitotic shake-off and released into HU-containing media for 6 h, cells were then released from HU for 150 minutes with EdU being added 30 minutes before collection. The EdU labeled DNA was then isolated and sequenced.