DNA-Seq of PAM library: MZ_Cpf1 in vivo_Zeo
Amplicons for next-generation sequencing were generated using two rounds of PCR. Equal amounts of the PCR amplicons were subjected to paired-end read sequencing using the Hiseq 2500 platform (Illumina, San Diego, CA, USA) at Novogene (Tianjin, China). Adaptors and low-quality reads were removed from the resulting 150-bp paired-end reads using Trimmomatic (version 0.36) . Reads were then mapped to the template using Bowtie2 (version 2.3.3). The total read count of each target region was above 20,000.