GSM3227554: pBIC_6; Mus musculus; RNA-Seq
Murine spleens were submitted to cellular disaggregation, followed by lysis of erythrocytes using ACK buffer. Then, single-cell preparations were stained with B220 and submitted to fresh cellular sorting in a FACSAria Ilu sorter (BD) after exclusion of dead cells with 7AAD. Total RNA was extracted from sorted cells using TRIzol (Invitrogen) as described in manufacturer´s protocol and quality control of extracted RNA was measured using Agilent Bioanalyzer. General transcriptome library preparation and massive sequencing were performed by BGI Tech (BGI, Shenzhen, China) using the Illumina HiSeq4000 platform with the paired-end 100-bp read option.
GEO Accession
GSM3227554