Trametes cingulata TCM6 genome sequencing using Roche 454 GS-FLX
1. DNA Fragmentation (Nebulization).     2. Fragment End Polishing.     3. Adaptor Ligation.     4. Library Immobilization.     5. Fill-In Reaction.     6. Single-Stranded Template DNA (sstDNA) Library Isolation.     7. sstDNA Library Quality Assessment and Quantitation.      All of the reagents used came within the GS-FLX Library Preparation Kit ordered from Roche.