EdUseq-HU/release of U2OS cells expressing normal levels of cyclin E: 14 h HU arrest and release for 60 min, no DRB was added (control)  (replicate 2)
U2OS cells expressing normal levels of cyclin E were synchronized by mitotic shake-off and released into HU-containing media for 14 h, cells were then released from HU for 60 minutes with EdU being added 30 minutes before collection. No DRB was added (control). The EdU labeled DNA was then isolated and sequenced.