Asian longhorned beetle transcriptome from midgut luminal contents
Gut contents was collected from the midguts of 5 third instar larvae actively feeding in the heartwood of a preferred host (sugar maple) and total RNA was extracted.  rRNA was depleted from the sample using MicrobEnrich and MicrobExpress.  20 ng of enriched RNA were amplified and converted into cDNA using Ovation RNA-Seq (Nugen). cDNA was utilized for library preparation with Illumina TruSeq DNA adapters and 40 million 130 by 130 nt PE reads were constructed using Illumina GAIIx.