GSM3732811: L826_relapse_AML; Homo sapiens; DNase-Hypersensitivity
DNase I (Worthington) digestion  was performed using ~5 million  patient sample cells using 8 units (presentation) or 14 units (relapse) for 3 min at 22 °C in a 1 mM CaCl2 supplemented buffer. Nuclear proteins were digested with 1 mg/ml Proteinase K overnight at 37 °C. DNaseI digestion products were size-selected on an agarose gel, cutting below 150 bp. High-throughput sequencing libraries were prepared using 10 ng of size-selected material, using the Kapa Hyperprep kit as per manufacturer's instruction. Libraries were sequenced with 50 bp single-end reads on an Illumina HiSeq 2500 sequencer according to manufacturer's instructions
GEO Accession
GSM3732811