GSM4263545: wt 30 min replicate 1; Arabidopsis thaliana; RNA-Seq
A pool of 10 intact whole seedlings was flash frozen and total RNA was extracted using TRI-Reagent. Polysaccharide precipitation was carried out using the double sodium acetate method. CAGE libraries were prepared as in (Takahashi et al., 2012) with a starting material of 5ug of total RNA. Phi-X spike-ins were added (30%) to each sequencing lane to account for low complexity of the 5' ends of the CAGE libraries
GEO Accession
GSM4263545