RNA-Seq of mycelia: before physiological maturation
Mycelia total RNA was extracted using TRIzol reagent (Life Technologies, New York, NY, USA) according to the manufacturer's protocol. The concentration, quality, and integrity of the extracted RNA samples were determined by agarose gel electrophoresis and a NanoDrop spectrophotometer (Thermo Scientific, USA). Six cDNA libraries were constructed using the Illumina TruSeq Stranded mRNA LT Library Preparation Kit (Illumina, USA) and then paired-end sequenced on an Illumina HiSeq 2000 platform by Shanghai Personal Biotechnology Co. Ltd. (Shanghai, China)