DNA_seq_env_enrichments_16s
DNA samples from Day 59 of the experiment were sent for sequencing the V4 region (515F 907R) of the bacterial 16S rRNA gene using paired-end sequencing (2   250 bp) on an Illumina Miseq instruments. PCR and sample preparation has been described in detail in Ren and colleagues (doi:10.3389/fmicb.2015.00022).