EdUseq-HU of U2OS cells expressing normal levels of cyclin E: 14 h after mitotic shake-off, not treated with DRB (control)  (replicate 1)
U2OS cells expressing normal levels of cyclin E were synchronized by mitotic shake-off and released into HU-containing media for 14 h with EdU being added for the last 9 h before collection. No DRB was added (control). The EdU labeled DNA was then isolated and sequenced.