GSM3905120: nanE.7.rep1; Escherichia coli; RNA-Seq
Cells were lysed in RNase-free TE buffer (30 mM Tris-HCl, 1 mM EDTA, pH 8.0) containing 1 mg/ml Lysozyme for 30 min at room temperature. Total RNA was prepared using an RNeasy Mini Kit (Qiagen). Genomic DNA was removed by RNase-free DNase (Qiagen). RNA quality was assayed using a NanoDrop and a 4200 TapeStation system (Agilent Technologies Inc.). Ribosomal RNA was depleted using a Ribo-Zero rRNA Removal Kit Bacteria (Illumina) and successful rRNA depletion verified by TapeStation. Ribosomal RNA was depleted using Ribo-Zero rRNA Removal Kit Bacteria (Illumina). The library was prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB). After quality and quantity assessment on the TapeStation the libraries were pooled equimolar. The resulting library pool was sequenced on a MiSeq sequencer (Illumina) performing a 2x 80 cycles paired-end run. stranded RNA-Seq with rRNA depletion
GEO Accession
GSM3905120