GSM2460758: s_H_MINUS_L_1; Mus musculus; RNA-Seq
poly(A) RNA was isolated using Dynabeads mRNA DIRECT Micro Kit Library preparation was performed according to the Ion Total RNA-Seq Kit v2 protocol (Life Technologies), with 16 cycles of PCR. Samples were barcoded, template-positive Ion PI Ion Sphere Particles (ISPs) were prepared using the ion one touch system II and Ion PI Template OT2 200kit v2 Kit (Life Technologies). Enriched particles were sequenced on a Proton sequencing system using  200bp version 2 chemistry. An average of 40  million reads per sample were generated.
GEO Accession
GSM2460758