GSM3594007: UC47noP9; Homo sapiens; RNA-Seq
Resting CD4+ T cells were sorted by HIV-1 RNA SortSeq into  single cells into RNA preserving buffer. RNA was reverse crosslinked with proteinase K and SDS under 50°C for 1 hour. RNA was extracted by Quick-RNA Microprep (Zymo Research) with a built-in on-column DNase treatment step. Total RNA from single cells was extracted followed by a ribosomal RNA depletion step using the SMARTer Stranded Total RNA-Seq Kit Pico Input Mammalian v2. Samples with different barcodes were pooled into one Hiseq4000 2x150 platform, targeting an estimated reads of 20-40 million per sample.
GEO Accession
GSM3594007