GSM4483630: Foxa3_4d; Mus musculus; ChIP-Seq
Sonicate the chromatin in nuclear extracts to fragments by ultrasonication (Covaris S220). Primary antibodies were used for immunoprecipitation in sonication supernatant by incubation at 4℃ overnight. Protein G Dynabeads (Life Technologies, 10004D) were used for pulling down antibody-chromatin complex followed by washing and eluting. Foxa3:5 micrograms for ChIP, from Santa Cruz, cat id sc-5361. Gata4:5 micrograms for ChIP, from Santa Cruz, cat id sc-1237. Hnf1a:5 micrograms for ChIP, from Santa Cruz, cat id sc-6547. CTCF:5 micrograms for ChIP, from Active Motif, cat id 61312. H3K27ac:5 micrograms for ChIP, from Abcam, cat id ab4729. H3K27me3:5 micrograms for ChIP, from CST, cat id 9733. MinElute PCR purification kit (Qiagen) was used for ChIP fragments collection. NEBNext Ultra DNA Library Prep Kit was used for ChIP-seq library preparation.
GEO Accession
GSM4483630