GSM4261424: sNuc_seq_Control; Mus musculus; RNA-Seq
mice were anesthetized, and the livers were perfused with perfusion media though the portal vein. Livers were subjected to dounce homogenization in cold swelling buffer (10 mM Tris pH 7.5, 2 mM MgCl2, 3 mM CaCl2, 2 U/ml Superase-In). After centrifugation at 400 g, the nuclei were extracted using lysis buffer (swelling buffer with 10% glycerol and 1% Igepal). After washing once with lysis buffer, the nuclei were resuspended in chilled (4 °C) nuclei wash and resuspension buffer (1X PBS, 1.0% BSA, and 0.2 U/μl RNase Inhibitor) with nuclei concentration of 1000 nuclei/ul. 10,000 nuclei were immediately proceeded with the 10X Genomics Chromium Single-Cell 3' according to the manufacturer's instructions. The libraries from control and HepDKO liver were constructed based on the 10X Genomics Chromium Single-Cell 3' manufacturer's instructions and sequenced by Nextseq 500.
GEO Accession
GSM4261424