mRNA_Seq analysis of HS0992
BCCAGSC mRNA-seq lite
EXPERIMENT_TYPE
mRNA-Seq
EXTRACTION_PROTOCOL
Trizol
EXTRACTION_PROTOCOL_MRNA_ENRICHMENT
SMART PCR cDNA Synthesis Kit
EXTRACTION_PROTOCOL_FRAGMENTATION
Bioruptor UCD-200
MRNA_PREPARATION_INITIAL_MRNA_QNTY
RIN 7
MRNA_PREPARATION_FRAGMENT_SIZE_RANGE
200-250bp
RNA_PREPARATION_5'_RNA_ADAPTER_SEQUENCE
NA
RNA_PREPARATION_3'_RNA_ADAPTER_SEQUENCE
NA
RNA_PREPARATION_REVERSE_TRANSCRIPTION_PRIMER_SEQUENCE
NA
RNA_PREPARATION_5'_DEPHOSPHORYLATION
NA
RNA_PREPARATION_5'_PHOSPHORYLATION
NA
RNA_PREPARATION_3'_RNA_ADAPTER_LIGATION_PROTOCOL
NA
RNA_PREPARATION_5'_RNA_ADAPTER_LIGATION_PROTOCOL
NA
RNA_PREPARATION_REVERSE_TRANSCRIPTION_PROTOCOL
SMART PCR cDNA Synthesis Kit
LIBRARY_GENERATION_PCR_TEMPLATE
cDNA
LIBRARY_GENERATION_PCR_POLYMERASE_TYPE
Phusion
LIBRARY_GENERATION_PCR_THERMOCYCLING_PROGRAM
98°C 30 sec, 10 cycle of 98°C 10 sec, 65°C 30 sec, 72°C 30 sec, then 72°C 5 min, 4°C forever
LIBRARY_GENERATION_PCR_NUMBER_CYCLES
10
LIBRARY_GENERATION_PCR_F_PRIMER_SEQUENCE
5' AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
LIBRARY_GENERATION_PCR_R_PRIMER_SEQUENCE
5' CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT
LIBRARY_GENERATION_PCR_PRIMER_CONC
0.4uM
LIBRARY_GENERATION_PCR_PRODUCT_ISOLATION_PROTOCOL
8% Novex TBE PAGE gel purification
LIBRARY_STRATEGY
mRNA-Seq