GSM2883910: 19169_WT-BM-xlink-jmjd1b_S2_L999_R1_001; Mus musculus; ChIP-Seq
ChIP on native chromatin from hematopoitic stem/progenitor cells was performed following  previously published protocols (M. Girardot et al., 2014. Nucleic acids research 42, 235-248)  with the following modifications using ChIP-grade Magna protein A/G beads (Cat#: 16-663, EMB Millipore). The isolated nuclei were incubated for 15 min at 37°C with 4 units/uL of Micrococcal nuclease (M0247, New England Biolabs) to produce mono/di-nucleosomes as verified by agarose gel electrophoresis. Nucleosomes were precipitated with control IgG, ChIP-grade anti-H4R3me2s (Cat#: Ab5823, Abcam) or anti-H3K9me2s (Cat#: 39375, Active motif). ChIP or input DNA was purified with Ampure XP beads (Cat#: A63880, Beckman Coulter). ChIP libraries were repard according to standard Illumina protocols. Transcriptome libraries were constructed with TruSeq RNA Sample Preparation Kit V2 (Illumia) with minor modifications.
GEO Accession
GSM2883910