Pair clone 1
RNA  libraries were prepared for sequencing using standard Illumina protocols.  First strand specific libraries were constructed using the Illumina TruSeq  Stranded mRNA Sample Prep Kit (RS-122-2101). The Poly-A tailed mRNA as  purified using the SeraMag Magnetic Oligo(dT) Beads. The mRNA fragmentation,  first strand cDNA synthesis, second strand cDNA synthesis, A tailing, adaptor  ligation and PCR amplification were performed according to the stranded mRNA  sample preparation guide of the kit. Sequencing was performed using an  Illumina HiSeq 4000 sequencer (DNA Sequencing Core of University of  Michigan).