GSM4663117: Lomustine, 135_18 (miRNA-seq); Homo sapiens; miRNA-Seq
Total RNA for analysis of miRNA was extracted from formalin-free alcoholic-based fixative FineFIX® and paraffin-embedded samples by MiRNeasy FFPE minikit (Qiagen, Venlo, Netherlands). RNA was quantified with Qubit 3.0 and Qubit RNA HS Assay kits (Applied Biosystems). 350 ng of total RNA were processed with the QiAseq miRNA kit (QIAGEN) according to manufacturer instructions. miRNA-seq libraries were  assessed by capillary electrophoretic analysis with the Agilent 4200 Tape station and sequenced with 1X75bp-reads on an Illumina NextSeq500.
GEO Accession
GSM4663117