GSM2884527: NHBE_repop_P118_7d; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNeasy Mini kit (Qiagen). RNA integrity was assessed using Bioanalyzer 2100 (Agilent Technologies) and RIN (RNA Integrity Number) values >8 were obtained for all samples except for sample NHBE_repop_P115_28d (RIN=7.5). RNA was quantified using the Quant-iT RiboGreen RNA Assay kit (Thermo Fisher). 560 ng total RNA/sample was used as input to create cDNA libraries using the TruSeq Stranded mRNA kit (Illumina) with dual indexing following standard instructions. Libraries were validated on the Fragment Analyzer platform (Advanced Analytical) using the Standard Sensitivity NGS Fragment Analysis kit. Sample libraries were pooled in equimolar concentrations and the concentration of the pool was verified using the Qubit fluorometer and the Quant-iT dsDNA High Sensitivity assay kit (Thermo Fisher). The library pool was diluted and denatured according to Illumina guidelines and sequencing was performed using a High Output 1 x 76 bp kit (Illumina) on an Illumina NextSeq500 platform.
GEO Accession
GSM2884527