RADseq of Thunnus thynnus
Extracted DNA was first digested with the ApeKI restriction enzyme (RE), which has a 5bp recognition site (5'GCWGC - 3'CGWCG), followed by ligation of standard Illumina sequencing adaptors with RE sequencing overhangs (Elshire et al. 2011). Libraries were sequenced at the Genomics Core Facility (KULeuven) using a HiSeq2500 (Illumina) 100 bp paired-end module, multiplexing 188 individuals on each sequencing lane with barcodes developed within the project.