Effects of Hypoxia on the Phylogenetic Composition and Species Distribution of Protists in a Subtropical Harbour
Samples collected onto 0.2 and 2um filters, DNA extracted using phenol-chloroform extraction method. PCR amplified using Euk82f (5’-GAAACTGCGAATGGTTCATTAAATCAG-3’) and EUK516r (5′-ACCAGACTTGCCCTCC-3′) . Sequencing was performed normally using 454 Roche protocol.