GSM1232712: livers group f; Rattus norvegicus; MBD-Seq
genomic DNA was isolated and fragmented by sonication; MBD fusion protein was incubated with fragmented DNA. Genomic DNA from rat livers was subjected to selection according to the methyl-CpG binding domain-based (MBD) protein protocol (DNA pooled from 6 individuals per group) and the resulting fragments sequenced in high throughput. Methylated DNA was captured with MethylCap kit (Diagenode). Approximately 10 ng of captured DNA was used for library preparation (ChIP-seq DNA sample Prep Kit, Illumina).
GEO Accession
GSM1232712