GSM3594044: DK1042_C RNA-seq; Bacillus subtilis; RNA-Seq
Following lysis, 2.5 A260 units were diluted in 700 µL H2O and denatured in 1% SDS. RNA was extracted once with an equal volume of hot acid phenol, once with an equal volume of room temp acid phenol, and RNA was precipitated with isopropanol. Precipitant was resuspended in H2O and 10 µg RNA was DNAse treated using 4 units of RNase free DNase I (Invitrogen AM2222) at 37°C for 30 min in a 20 μL reaction volume. RNA was precipitated in isopropanol, resuspended in H2O. Libraries were prepared by the Indiana University Center for Genomics and Bioinformatics using the ScriptSeq RNA-library kit (Illumina SSV21124).
GEO Accession
GSM3594044