SMRT-OTS of human: HEK293 DNA
A SMRT-OTS library  was constructed with a modified version of the protocol described by Tsai et  al (2017), BioRxiv. Instead of enzymatic shearing and genomic complexity  reduction, random shearing, to 8 kb, with Megaruptor 2 (Diagenode) was used.  The sample was also size-selected using the Blue Pippin system (Sage Science)  before going into the Cas9 digestion. Guide RNAs targeting the ATXN10, MMP14  and NEK1 genes were used in the experiment. Sequencing was performed on the  Sequel system using 3.0 sequencing and binding chemistry and a 10 hr movie  time.