Whole genome bisulfite sequencing of Homo Sapiens: HUES8 human embryonic stem cells with inactivating mutations in TET1, TET2 and TET3
Genomic DNA was isolated from HUES8 WT and HUES8 TKO hESCs. Genomic DNA was sheared using E220 focused-ultrasonicator to 250-350bp fragments. After end-repair and TruSeq adapter (Illumina) ligation the DNA-libraries were denatured followed by treatment with bisulfite.