GSM3069969: MNase_YPD30_nhp10-B_3333_40U.sgr.gz; Saccharomyces cerevisiae; MNase-Seq
Cells were spheroplasted with zymolyase, then digested with MNase and exonuclease, treated with Proteinase K and RNase A, extracted with phenol:chloroform:IAA and ethanol precipitation. Samples were generally digested with 40 units of MNase, but a few samples also treated with 20U or 80U for control experiments. In all experiments, digested DNA was run on a low-melt agarose gel, the mononucleosomal band was gel-purified, and this DNA was sequenced. Libraries prepared with Nugen Ovation Ultralow System V2 (catalog # 0344-32).
GEO Accession
GSM3069969