GSM3904409: unaffected individual 3; Homo sapiens; RNA-Seq
Skin fibroblasts from individuals V-3 and VI-1 from family 1 and four different healthy age matched controls were cultured to 80% confluence in T175 flasks, before lysis with TRIzol™ Reagent (Invitrogen®, 15596026) and RNA isolation with RNeasy mini kit (Qiagen®, 74106). Strand-specific mRNA-seq libraries for the Illumina platform were generated with a poly-A selection and sequenced at GenomeScan (GenomeScan, Leiden, The Netherlands). Fastq files from forward and reverse reads were aligned to reference genome hg38 with the STAR aligner tool (v.2.4.2a) (https://www.ncbi.nlm.nih.gov/pubmed/23104886) and quantified per gene with HTSeq using hg38 exon coordinates (release 86). Count data were used for differential gene expression using the edgeR package in R.
GEO Accession
GSM3904409