GSM4483641: 0d_rep2; Mus musculus; Hi-C
DNA was cut with restriction enzyme DpnII (NEB), and the end of the restriction fragment was labeled with biotin-14-dATP (Life Technologies). Then, the biotinylated products were used for proximity ligation. After the reversed cross-linking of the ligated biotinylated DNA, it was purified and cut into about 400bp fragments by ultrasonic treatment (Covaris S220). The ligation products with biotin-labeled ligaments were isolated by using MyOne streptavidin anti-biotin protein C1 Dynabeads (Life Technologies) and followed by Illumina double-ended sequenced ligation. Biotinylated fragments were pulled down with streptavidin beads and amplified using 11-12 PCR cycles to enrich for physically interacting sites.
GEO Accession
GSM4483641