GSM4265545: ControlOligo9h; Homo sapiens; RNA-Seq
At the endpoint, media was aspirated and cells were lysed in 300 uL RLT buffer (RNeasy extraction kit, Qiagen) and total RNA was extracted. RNA integrity was verified by an RNA 6000 Nano Chip, using BioAnalyzer and 2100 Expert software (Agilent Technologies). rRNA was depleted from the total RNA by hybridization to bead bound rRNA probes. This was followed by fragmentation, first and second strand synthesis for library construction, adapter ligation, PCR amplification and finally sequencing on Illumina HiSEq 2000 platform.
GEO Accession
GSM4265545