[TEMPORARY] Non-normalized cDNA prepared from mixed ovarian and embryonic RNA of Parhyale hawaiensis
Non-normalized cDNA was prepared using a custom protocol.  For details, see <PMID OF STUDY>. Briefly, total RNA from mixed staged embryos and ovaries was used to generate cDNA using an "interrupted" poly-A primer and an MmeI site in order to partially remove the polyT primer during MmeI digestion.  Note that the following adapters were used during cDNA synthesis and *must* be screened before assembly:  >PD243Mme-30TC-3'Primer-undigested ATTCTAGAGCGCACCTTGGCCTCCGACTTTTCTTTTCTTTTTTTTTCTTTTTTTTTTVN >PD243Mme-30TC-3'Primer-digested TTTTTTTTTTVN >PD243Mme-30TC-3'Primer-minus-polyT ATTCTAGAGCGCACCTTGGCCTCCGACTTTTC >PD242-5'-1st-strand-primer-undigested AAGCAGTGGTATCAACGCAGAGTGGCCACGAAGGCCGGG >5'-amplification-primer_clontech-undigested AAGCAGTGGTATCAACGCAGAGT >5'-primer-digested AGGCCGGG