GSM2463822: hSTAT5B, replicate 3; Mus musculus; RNA-Seq
Lymph nodes were isolated and kept on ice, single-cell suspensions were inclubated with CD8 antibody and magnetic bead, isolated CD8 T-cells were flash-frozen in Trizol reagent, RNA was harvested 1µg of total RNA per sample was taken for cDNA library synthesis using SENSE mRNA-Seq Library preparation kit according to the manufacturers recommendations. 50 bp single-end RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM2463822