Sequencing of 16S rRNA: compost sample
Briefly, the raw reads were demultiplexed, quality-trimmed with Trimmomatic and merged with FLASH as follows: (i) reads with a quality score < 20 or a length < 50 bp were truncated. (ii) Reads shared overlap longer than 10 bp were merge to a sequence according to their overlap relationship. (iii) The Sequences were matched to specific samples based on the unique barcoded primers for each sample. After the singletons and chimeric sequences were discarded, representative sequences were obtained during operational taxonomic units (OTUs) cluster analysis of non-repetitive sequences into at a 97% similarity level by USEARCH. Finally, mapping all optimized sequences to the representative sequences to generate an overall OTU outputs at a similarity level of 97%.