10x scRNAseq of CD4+hCD2- Th of tolerized grafts
Single cells were purified by FACS sorting before library preparation and single-cell libraries were prepared with the Chromium Single Cell 3 Reagent Kits v2 (10x Genomics) as per manufacturers instructions. Sequencing libraries were prepared by fragmentation, end-repair, ligation with indexed adapters and PCR amplification using the Chromium Single Cell 3 library kit v2 (10x Genomics).