wild type partheno-sporophyte base replicate 1
Individuals were cultivated in 320 mm round petri dishes in natural sea water supplemented with 0.01% Provasoli enrichment under low light conditions. Individuals had not produced uprights, nor unilocs or plurilocs. Tissue (whole thalli) was harvested 3 weeks after gamete release (at 16h30 to 18h30 in the evening) and frozen in liquid nitrogen. The cultures used to harvest basal tissue were not the same as those from which the uprights were harvested.