GSM4262360: Donor_207B_HSCMPP_BM_340; Homo sapiens; RNA-Seq
HSC/MPPs were purified by flow cytometry sorting from CD34+ enriched (magnetic selection) bone marrow or spleen samples as CD19- CD34+ CD38- CD45RA-. Single cell were directly sorted into lysis buffer according to the Smart-seq2 protocol published by Picelli et al., Nat. Protocol 2014. RNA libraries were prepared for sequencing using standard SmartSeq2 protocols and the Illumina Nextera XD DNA sample preparation protocol.
GEO Accession
GSM4262360