GSM1666978: R1.CE.8E.ChIP; Caenorhabditis elegans; ChIP-Seq
Whole embryo lysates were clarified from sonication and PolII-DNA complex were isolated with antibody. The ChIP-Seq libraries from two wild-type 8E stage Pol II ChIP and one wild-type bean stage Pol II ChIP were generated by using Apollo 324 System and PrepX ILM DNA Library Kit from IntergenX. After adaptor ligation, the input and ChIP DNA were enriched by PCR amplification using Phusion DNA Polymerase (New England Biolab) and Illumina universal PCR primer
GEO Accession
GSM1666978