GSM3069707: Wt Skull 2; Mus musculus; RNA-Seq
RNA was extracted from isolated tissue samples using the RNeasy Lipid Tissue Mini Kit (Qiagen Cat. NO 74804) following the manufacturer's protocol. Quality of extracted mRNA was assessed using DNA Analysis ScreenTape (Agilent Technologies, Santa Clara, CA) to ensure that it was of sufficient quality for library production. Following validation of extracted mRNA, cDNA libraries were generated using the Illumina TruSeq Stranded mRNA Sample Prep Kit (Illumina, San Diego, CA). RNA-seq using the Illumina HiSeq4000 Platform and single end reads (1x151).
GEO Accession
GSM3069707