GSM4261885: DSETX_ChIP-Seq_Replicate 1; Homo sapiens; ChIP-Seq
Cells were lysed in ChIP cell lysis buffer (5 mM HEPES pH 8.0, 85 mM KCl, 0.5% NP-40 and protease inhibitors) and incubated 5 min on ice. Nuclei were pelleted by centrifugation at 3,900 g for 5 min at 4°C. Nuclei were then resuspended in ChIP nuclear lysis buffer (50 mM Tris-HCl pH 8.1, 10 mM EDTA, 1% SDS and protease inhibitors) and sheared to fragments between 200-500 bp using a bioruptor sonication system (Diagenode). Sonicated chromatin was clarified by centrifugation at 20,000 g for 15 min at 4°C. The clarified chromatin was incubated with the RNAPII (4H8) antibody-conjugated Protein A Dynabeads (Invitrogen, ThermoFisher) overnight at 4°C. Libraries were prepared according to Illumina's instructions.
GEO Accession
GSM4261885