GSM4266010: WTX_REF, 10x genomics (lung mesenchyme); Mus musculus; RNA-Seq
Lungs were perfused with cold PBS and instilled with collagenase I. Lungs were removed while trachea was clamped. The lungs were cleaned from non-respiratory tissue and minced. Following depletion of erythrocytes, mesenchymal cells were sorted as Dapi–/Epcam–/CD45–/CD31–/Pdgfra+/Sca1–/Npnt+ TAP-seq, 10x genomics based Libraries were prepared according to DropSeq protocol (Macosko Cell 2015) or 10X Genomics single-cell RNAseq v2 (Chromium 3' Reagent Kits v2 User Guide Rev C "CG00052") for whole transcriptome or TASC-seq protocol for targeted transcriptome readouts.
GEO Accession
GSM4266010