GSM2113685: 1145F47_WSBEiJ; Mus musculus; RNA-Seq
Inguinal fat pads were dissected and stored in RNAlater at -80C). Total RNA was isolated using the Trizol Plus RNA extraction kit (Life Technologies) with on-column DNase digestion. Following the Illumina TruSeq standard protocol, indexed mRNA-seq libraries were generated from 1μg total RNA followed by QC and quantitation on the Agilent Bioanalyzer and the Kapa Biosystems qPCR library quantitation method.
GEO Accession
GSM2113685