GSM2733395: mOSN-Lhx2KO_RNAseq_rep2; Mus musculus; RNA-Seq
Crosslinked-ChIP: Chromatin from formaldehyde cross-linked cells was sheared on a Covaris S220 Focused-ultrasonicator to a size range of 200-500bp. Approximately 1-2ug of sheared chromatin was used for immunoprecipitation with antibodies, and precipitated DNA was column purified. Native-ChIP: Native chromatin was digested with Micrococal Nuclease to predominantly mono and di-nucleosome sized fragments. Nucleosomal DNA was immunoprecipitated with antibodies and column purified. ATAC: Cells were lysed and then transposed with Nextera Tn5 transposase (Illumina). Transposed DNA was column purified using a Qiagen MinElute PCR cleanup kit. RNA: Cells were lysed in Trizol-LS (ThermoFisher) then RNA was extracted and treated with Turbo-DNase (ThermoFisher). Crosslinked-ChIP: ChIP-seq Libraries were prepared using a Nugen Ultralow V2 kit. Native-ChIP: ChIP-seq Libraries were prepared using a Nugen Ultralow V2 kit. ATAC: Transposed DNA was amplified using barcoded primers and NEBNext High-Fidelity PCR Master Mix. RNA: RNA-seq libraries were prepared using a TruSeq Stranded Total RNA with Ribo-Zero Gold Set B kit (Illumina RS-122­2302).
GEO Accession
GSM2733395