M82CBIOREP2
Small RNAs libraries were prepared using the NEBNext Small RNA Library Prep (New England Biolabs). In brief, three biological replicates each of 1 month old tomato leaf RNA were prepared using 1 g of total RNA per sample. After preparation, size selection of libraries was performed using BluePippin 3% agarose cassettes (Sage Science). Each library was barcoded, pooled, and sequenced using a single NextSeq 500/550 High Output Kit v2 (75 cycles).