codon optimized CAD: Corynebacterium glutamicum ATCC 13032
The sequencing reaction is a BigDye Terminator v3.1 cycle sequencing kit from Applied Biosystems.PCR reaction is performed using BIO-RAD's DNA engine Tetrad 2 Peltier Thermal Cycler.Ask the customer.Applied Biosystems Reacts in the recommended way and has dNTPs and reactants that do not participateABI 3730xl DNA analyzer.