mRNAseq of Paroedura stumpffi: adult female blood
The total RNA samples from two females of P. stumpffi, two females of P. oviceps and a single female of P. lohatsara were sent to GeneCore (EMBL, Heidelberg, Germany) for library construction. The barcoded stranded mRNA-sequencing libraries were prepared using the Illumina TruSeq mRNA v2 sample preparation kit (Illumina, San Diego, CA, USA) with poly-A mRNA enrichment, implemented on the liquid handling robot Beckman FXP2. Obtained libraries were pooled in equimolar amounts; 1.8 pM solution of this pool was loaded on the Illumina NextSeq 500 sequencer and 85bp were sequenced uni-directionally.