Fitness profiling under stress treatment conditions (TBZ, CPT, HU, UV)
Cells were lysed in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0) by beating   with glass beads in a FastPrep-24 Instrument (MP Biomedicals). Genomic DNA was   extracted using the MasterPure Yeast DNA Purification Kit (EPICENTRE   Biotechnologies). The barcodes were amplified with Ex Taq HS DNA polymerase   (TaKaRa) through 30 cycles of 20 s at 94°C, 20 s at 53°C, and 20 s at 72°C. For uptags,   the forward primer (upf-X) was 5’-  CACGACGCTCTTCCGATCTXXXXGAGGCAAGCTAAGATATC-3’, the reverse   primer (upr) was 5’-AGCAGAAGACGGCATACGAGCCTTACTTCGCATTTA-3’. For dntags, the forward primer (dnf-X) was 5’-  CACGACGCTCTTCCGATCTXXXXCCAGTGTCGAAAAGTATC-3’, the reverse   primer (dnr) was 5’-AGCAGAAGACGGCATACGATTGCGTTGCGTAGG-3’.   “XXXX” in the forward primer sequences denotes the 4-nt multiplex indexes. The PCR   products were diluted 200 folds and used as templates for another round of PCR to add   sequences needed for Illumina sequencing. The forward primer (seqf) was 5’-  AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCC  GATCT-3’, the reverse primer (seqr) was 5’-CAAGCAGAAGACGGCATACGA-3’.   The cycling parameters were: 20 cycles of 20 s at 94°C, 20 s at 56°C, and 20 s at 72°C.   The second round PCR products were mixed together in equal molar ratios and gel   purified to use as the Illumina sequencing template. Standard single-end sequencing   primer was used and 42 cycles of sequencing were carried out with an Illumina Genome   Analyzer II.