GSM3675286: hela.2.OE.sfpq.IP; Homo sapiens; OTHER
Total RNA was first extracted from HeLa cells by Trizol reagent, The polyA RNA was then purified with oligo d(T)25 magnetic beads PolyA RNA was fragmented by NEBNext Magnesium RNA Fragmentation Module. A portion of fragmented RNA was reserved as m6A input sample and the left was incubated with anti-m6A antibody and eluted to get the m6A IP. Both input and m6A IP samples were prepared for next-generation sequencing library by standard protocol of NEBNext Ultra II Directional RNA Library Prep Kit for Illumina.
GEO Accession
GSM3675286