Illumina sequencing of sample "SAT Cross: DHTu G0"; library "DHTu2 - 200NOPCR1" for study accession ERP000232.
noPCR. This library contains a reasonably high level of chimearism, so we do not suggest it is used. At the time of selecting SNPs for the SAT map this library yielded the only sequence we had for the DHTu2 fish. We had 25x coverage for the DHAB fish, and predicted SNPs between AB and the Tubingen reference sequence (Zv8), so we used the ~4x coverage from this library to confirm positions where the DHTu2 fish agreed with the reference. It was not used in subsequent analysis as it was superseded by sequence from higher quality libraries.