GSM2559356: 07_P47_CTX_cKO_DJ4; Mus musculus; RNA-Seq
Brain regions were dissected out and immediately flash frozen in liquid nitrogen. Total RNA was then purified from these samples, using an mRNeasy kit (Qiagen), following the manufacturer's instructions. mRNA was purified via poly(A) selection from 2ug of total RNA. Purified mRNA was then utilized to generated cDNA libraries using previously published protocols (Takahashi et al., 2015). Pooled libraries (at a final concentration of 2nM) were sequenced on a NextSeq 500 sequencer (Illumina) by the McDermott Sequencing Core at the UT Southwestern Medical Center.
GEO Accession
GSM2559356