GSM1667181: shNT, hypoxia, replicate 1; Homo sapiens; RNA-Seq
Total RNA was extracted using TRI reagent according to manufacturer's instructions and quality assessed using Bioanalyzer RNA Pico chips (Agilent). PolyA RNA was purified using the Dynabeads mRNA Direct micro kit (Life Technologies) according to manufacturer's instructions and ribosomal RNA contamination assessed using Bioanalyzer RNA Pico chips. Libraries for Ion Torrent sequencing were prepared using the Ion Total RNAseq v2 kit (Life Technologies), according to manufactorer's instructions. Yeild and insert-size distribution were measured using Bioanalyzer High Sensitivity DNA chips (Agilent). The final Ion Torrent RNAseq libraries were subjected to an additional size-selection for 120-200bp on a Blue Pippin (Sage Science). Ion Torrent template preparation was carried out using the Ion PI Template OT2 200 Kit v2 and library sequencing carried out on an Ion Torrent Proton sequencer using the Ion PI Sequencing 200 Kit v2 (Life Technologies) according to manufacturer's instructions.
GEO Accession
GSM1667181