GSM2732720: Mitf_3; Mus musculus; RNA-Seq
Melanocyte stem cells were isolated from dissociated dermis using flow cytometry, and subesquently harvested in TRIzol reagent. Total RNA was purified using the Directzol RNA MiniPrep Kit (Zymo), and quantified on a Qubit fluorometer. RNA quality for RNA sequencing was assessed by Bioanalyzer (Agilent), and only samples with RIN scores >7.5 were used. mRNA libraries were constructed from ~200 ng total RNA using the Illumina TruSeq RNA Sample Prep Kits, version 2.  The resulting cDNA was fragmented using a Covaris E210. Library amplification was performed using 16 cycles.  Unique barcode adapters were applied to each library.
GEO Accession
GSM2732720