GSM4160746: Riboseq.mouse.testis.wt.adult.HarringtonineUntreat.rep1; Mus musculus; OTHER
Total RNAs were extracted using mirVana miRNA Isolation Kit (ThermoFisher, AM1560), and rRNAs were depleted from total RNAs with complementary DNA oligomers (IDT) and RNaseH (Invitrogen, Waltham, MA, USA). Ribo-seq: Testes samples were lysed, RNase T1 & A treated and loaded on sucrose gradients, and 80S monosomes were recovered for library construction. The 3'-adapter is TGGAATTCTCGGGTGCCAAGG and has been trimmed in the raw data.
GEO Accession
GSM4160746