GSM2112894: Myotubes; Homo sapiens; RNA-Seq
Total RNA was extracted using Trizol solution following the manufacture’s instruction. The quality of total RNA was checked by Nanodrop (260/280=1.9-2.1, 260/230>2.0) and BioAnalyzer 2000 (RIN>7). The cDNA library from Poly(A) RNA for massive parallel sequencing was prepared from each 500 ng total RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module and Ultra Directional RNA Library Prep Kit for Illumina. The produced cDNA library was in 400-1000 bp size range including the adaptor sequences.
GEO Accession
GSM2112894