GSM4315212: IP4; Drosophila melanogaster; RIP-Seq
RNA were harvested using Trizol reagent and mRNA were isolated by two rounds of binding to oligo (dt)25 magnetic beads (NEB). mRNA was fragmented with RNA fragmentation solution (Ambion). Samples IP1, IP2, IP3, IP4 and ctr were immunoprecipitated using anti-m6A antibody. Sample frag-mRNA was purified with RNAClean XP beads (Beckman Coulter). Library preparation for IP1, IP2, IP3, IP4 and ctr samples was performed as previously described in (Sutandy et al., 2016). Libraries were sequenced on an Illumina NextSeq500. Library preparation for frag_mRNA was performed using the NEBNext Ultra Directional RNA Library Prep Kit.
GEO Accession
GSM4315212