GSM4411613: Sample 6_RNA-Seq_high_cortisol; Bos taurus; RNA-Seq
Blood samples collected in the PAX gene Blood RNA Tubes (PreAnalytiX GmbH, Switzerland) were frozen 4 h after the collection and stored at -80 °C until the RNA isolation. Prior to RNA isolation, blood samples were thawed at +4 °C for at least 12 h. RNA was isolated according to PAX gene Blood RNA Kit (PreAnalytiX GmbH, Switzerland) protocol. Quantity and quality of RNA was analysed with Agilent 2100 Bioanalyzer (Agilent Technologies, United States) before sequencing and the RNA integrity number (RIN) score was  ≥ 7 for all the samples. Since the RNA-Seq is highly reproducible within a large dynamic range of detection and provides an accurate estimation of RNA concentration in peripheral whole blood, globin depletion was avoided, as it could significantly reduce the amount and quality of isolated RNA. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM4411613