GSM2045693: β1-cKO-2; Mus musculus; RNA-Seq
RNA was extracted from pooled samples of 75 (C57BI/6) or 30 (β1-cKO) mouse lenses, using the SV total RNA isolation System (Promega, Madison, WI) according to the manufacturer's recommended protocol. mRNA was purified from the total RNA samples using poly-T oligo attached magnetic beads. Then, it was converted to a library of template molecules for cluster generation and DNA sequencing according to the Illumina® TruSeq™ RNA Sample Preparation Kit v2. Afterwards, RNAseq was performed on Illumina® HiSeq 2000 (Illumina Inc., Madison, Wisconsin), with 50-cycle single-end (50bp) reads.
GEO Accession
GSM2045693