WGS of Potato Leaf Heirloom Tomato Cultivars
DNA was extracted using GeneJET Plant Genomic DNA Purification Mini Kit (Thermo Scientific, Waltham, MA, USA) from plants grown for a month and DNA-Seq libraries were prepared based on BrAD-seq (Townsley et al., 2015) with the following modifications: After DNA fragmentation with Covaris E220 (Covaris, Inc. Woburn, MA, USA), the fragmented DNA was end-repaired, A-tailed, and adapter ligated with Y-adapter. Enrichment PCR was then performed with the adapter ligated product as described Townsley et al., 2015. After final library cleanup with AMPure beads (Beckman Coulter, Brea, CA, USA), DNA-Seq libraries were sequenced at Novogene (Novogene, Inc. Sacramento, CA, USA) using the HiSeq 2500 platform (Illumina Inc. San Diego, CA, USA).