16S ribotyping of Mus musculus: feces
The feces of mice that received streptomycin treatment on day -2 and various treatment (COP (TP114::Kill1 probiotic donors), TP114 probiotic donors or PBS) on day 0 were sampled daily. The DNA was extracted using ZYMOBIOMICS DNA prep kit and the V3-V4 region of the 16S rDNA was amplified. The amplified 16S region was then sequenced with paired-end 300 bp on an Illumina MiSeq instrument.