Illumina paired exome sequencing of Alternating Hemiplegia of Childhood (AHC) disease study sample 11
Sequenced by BGI: the qualified genomic DNA are randomly fragmented by Covaris, resulting in DNA fragments with a base pair peak at 150 to 200bp, and then adapters are ligated to both ends of the fragments. The adapter-ligated DNA fragments are purified by the Agencourt AMPure SPRI beads and fragments with an insert size of about 200bp are excised. Extracted DNA are amplified by ligation-mediated polymerase chain reaction (LM-PCR), purified, and hybridized to the SureSelect Biotinylated RNA Library (BAITS) for enrichment. Hybridized fragments are bound to the strepavidin beads whereas non-hybridized fragments are washed out after 24hrs. Captured LM-PCR products are subjected to Agilent 2100 Bioanalyzer to estimate the magnitude of enrichment. Each captured library is then loaded on Hiseq2000 platform.