GSM2559507: PIWIL1-IP small RNA-seq rep2; Mus musculus; RIP-Seq
Testis was dissected and frozen. For PIWIL1-IP samples, immunopurification PIWIL1 was performed using testis lysates followed by RNA isolation using Isogen (Nippongene), and the size fractionation was obtained by gel-purification from total RNA, using the Small RNA Gel Extraction kit (TaKaRa). RNA-seq samples were prepared by total RNA isolation using Isogen (Nippongene). small RNA-seq libraries were prepared using NEBNext Small RNA Library Sample Prep Set (New England BioLabs), and RNA-seq libraries were prepared with the directional mRNA-seq library prep protocol using TruSeq Standard mRNA Sample Prep Kit (Illumina).
GEO Accession
GSM2559507