RNA-seq gill
A total amount of 1.5 g RNA of sample was used as input material for the RNA-seq library preparations. Sequencing libraries were generated using NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA) following manufacturers recommendations index codes were added to attribute sequences to each sample. The quality of prepared library was assessed on the Agilent Bioanalyzer 2100 system.