GSM2886571: M. smegmati WT; Mycolicibacterium smegmatis MC2 155; RNA-Seq
Total RNA was isolated using RNeasy mini kit (Qiagen, Germany). Strand-specific libraries were prepared. ribosomal RNA was removed from total RNA using Ribo-Zero rRNA removal beads. Following purification, the mRNA is fragmented into small pieces. The cDNA fragments afterwards through an end repair process, the addition of a single 'A' base, and then ligation of the adapters. The products are then purified and enriched with PCR to create the final cDNA library.
GEO Accession
GSM2886571