GSM3523341: WX2_mRNAseq_run333; Streptococcus pneumoniae D39; RNA-Seq
RNA was isolated from cells via the Fastprep blue kit (MPBiomedicals) For samples 1-6 and 8-9, the library was generated by removing DNA by DNase treatment, depleting rRNA with Ribo-Zero rRNA removal kit for gram-positive bacteria and subsequently utilizing the ScriptSeqTM v2 RNA-Seq Library Preparation kit (Epicenter).  For samples 7 and 10, the same protocol excpet for TruSeq® Stranded RNA kit (Illumina Inc.) was used to prepare sRNA libraries using 145 - 500 bp size selection.
GEO Accession
GSM3523341