GSM4261758: 180 minute incubation RepA; Neisseria gonorrhoeae 1291; RNA-Seq
RNA was extracted with TRIzol rRNA depletion with Epicentre MetaScript Ribo-Zero rRNA removal kit.  cDNA was generated with the NuGen Ovation RNA-Seq system, v2 and was sheared using a Covaris E220 to give an average fragment size of 300 bp.  500 ng of each cDNA were used to generate indexed libraries using the KAPA Hyper Prep Kit (KAPA Biosystems, Wilmington, MA, Cat. No. KK8500).  The molar concentrations of the indexed libraries were measured using the 2100 Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA) and combined equally into a pool for sequencing. The concentration of the pool was measured using the Illumina Library Quantification Kit (KAPA Biosystems, Wilmington, MA) The sequencing pool was loaded into a single lane of an Illumina HiSeq 2500 flow cell running 150 bp paired-end SBS v4 sequencing chemistry
GEO Accession
GSM4261758