Metagenomic Sequencing of Spiroplasma and Wolbachia naturally infecting Drosophila melanogaster
For sequencing, heads were first removed by immersing whole flies in liquid Nitrogen, and vigorous shaking on a metal sieve. The headless specimens were immediately subjected to phenol-chloroform extraction. Nanopore library preparation was performed using the Ligation Sequencing kit 1D (SQK-LSK108), following precautionary measures to ensure long read lengths. The library was then sequenced on MinION sequencing device (MIN-101B) with an SpotON Flow Cell Mark 1 (R9.5- FLO-MIN107.1). Base Calling was performed using Albacore version 2.0 .