RNA-seq of different tissues of Brassica nigra var Sangam
Illumina based RNA-seq libraries was developed with 10g of total RNA, as described earlier by Paritosh et al (2013). Quality of the developed library was checked on an Agilent 2100 Bio analyzer. The Illumina based libraries of size 200-250bp were sequenced as paired end reads on the Genome Analyzer IIx instrument (GAIIx, Illumina).