Chinese Jiang-flavor daqu metatranscriptomes
Before metatranscriptomic library construction, depletion of ribosomal RNA was performed for prokaryotes, and mRNA was isolated using magnetic beads with Oligo (dT) for eukaryotes. The isolated mRNA was first fragmented and then used as template for the following first-strand cDNA and second-strand cDNA synthesis with random primers. Short cDNA fragments were purified and resolved with EB buffer for end reparation and adding poly(A). Thereafter, the short cDNA fragments were ligated to sequencing adapters, and suitable size cDNA fragments were purified as templates for PCR amplification. RNA sequencing of the library was performed using HiSeqTM 2000 platform