MeDIP-Seq analysis of HS1821
Costello Lab MeDIP protocol
EXPERIMENT_TYPE
DNA Methylation
EXTRACTION_PROTOCOL
Costello Lab MeDIP protocol
EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR
Diagenode Bioruptor
EXTRACTION_PROTOCOL_SONICATION_CYCLES
25 min
MeDIP_PROTOCOL
Costello Lab MeDIP protocol
MeDIP_PROTOCOL_DNA_AMOUNT
5-15 ug
MeDIP_PROTOCOL_BEAD_TYPE
Protein A/G
MeDIP_PROTOCOL_BEAD_AMOUNT
100 ul
MeDIP_PROTOCOL_ANTIBODY_AMOUNT
1ug /ug of gDNA
MeDIP_ANTIBODY
Methylcytidine
MeDIP_ANTIBODY_PROVIDER
Eurogentec
MeDIP_ANTIBODY_CATALOG
BI-MECY-0100
MeDIP_ANTIBODY_LOT
80604
LIBRARY_STRATEGY
MeDIP-Seq