GSM2807800: 24023_benign_prostate_TABseq; Homo sapiens; OTHER
Genomic DNA was extracted using the AllPrep DNA/RNA kit (Qiagen, USA) β-Glucosetransferase (GT)-mediated 5hmC protection and TET1-mediated 5mC oxidation were performed using the 5hmC TAB-seq Kit (WiseGene, USA, cat no K001).  Bisulphite treatment of TET1-oxidised DNA followed by the library preparation was performed using the TrueMethyl Whole-Genome kit from Cambridge Epigenetix according to the manufacturer's instructions. Paired-end 150bp sequencing was performed for each library on the Illumina HiSeqX platform using the HiSeq X™ Ten Reagent Kit v2.
GEO Accession
GSM2807800