GSM4266000: TASC_DEEP, panel 3 (cell type mix); Mus musculus; RNA-Seq
Cells were collected using centrifugation and subjectd to single-cell RNAseq using 10X Genomics. For neutrophils, femurs, tibiae, hips and spines were dissected and cleaned from surrounding tissue. Bones were crushed in cell suspension medium and dissociated cells were filtered, red blood cell lysis was performed and single viable cells were isolated using forward/sideward scatter and DAPI dye exclusion, and CD11b+/Ly6G+ neutrophils were isolated. TAP-seq, 10x genomics based Libraries were prepared according to DropSeq protocol (Macosko Cell 2015) or 10X Genomics single-cell RNAseq v2 (Chromium 3' Reagent Kits v2 User Guide Rev C "CG00052") for whole transcriptome or TASC-seq protocol for targeted transcriptome readouts.
GEO Accession
GSM4266000