Pyronema confluens PacBio RS sequencing
Genomic DNA was prepared as described by Traeger et al. (PLoS Genet 9: e1003820). For PacBio sequencing, DNA was treated with 1/10 volume RNase cocktail (RNase A + RNase T1, Ambion) in restriction digest buffer Tango (Fermentas) for 1 h at 37 °C. Afterwards, small nucleic acids and nucleotides were removed by filtration with Amicon Ultra 30k columns. 40 µg genomic DNA were used for PacBio RS library preparation (target size 2 kb) at GATC Biotech (Konstanz, Germany).