To study the effects of type-2 diabetes mellitus (T2DM) and hypoglycemic therapy on the salivary microbiome in periodontitis and identify the potential salivary micro-biomarkers for the early warning of T58DM.
TruSeq DNA PCR-Free Sample Preparation Kit (Illumina, San Diego, CA, USA) was used for library preparation. The constructed library was quantified by Qubit and qPCR for quality control and sequenced by HiSeq2500 PE306 (Illumina)