GSM2551686: LIJ2.2; Homo sapiens; RNA-Seq
Drop-seq samples were prepared following the published protocols (Macosko et al., 2015). For single-cell index sorting samples, we followed a modified SMART-Seq2 protocol (Picelli et al., 2013), with the exception that a 12-base cell barcode was included in the 3'-end RT primer. Libraries were prepared using the Nextera XT DNA sample prep kit (Illumina) using a custom Read 1 primer (GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC, IDT).
GEO Accession
GSM2551686