De novo genome sequencing with PacBio of watermelon 97103: young leaves
The high molecular weight genomic DNA was extracted from the ground powder of the frozen sample with the cetyltrimethylammonium bromide (CTAB) method (Murray & Thompson, 1980) and purified using magnetic bead (BioVision, Inc., USA). DNA sample of watermelon line 97103 was randomly sheared to fragments with an average size of 20 kb by using Covaris g-TUBE (Covaris Inc., USA) and the sheared DNA was used to construct the PacBio sequencing library following the standard SMRT bell construction protocol (Pacific Biosciences Inc., USA). The quality of the library was assessed by an Agilent 2100 Bioanalyzer High Sensitivity Kit (Agilent Technologies Inc., USA). The PacBio SMRT library were sequenced on a PacBio Sequel platform.