RNA-seq of populus tomentosa cells exposed to light for 30 days
Total RNA was isolated from samples using TRIzol reagent according to the manufacturers protocol (Invitrogen). After confirmation of RNA quality, the samples were sequenced using a BGISEQ-500 Analyzer (BGI Genomics, Shenzhen, China). The raw reads were data-filtered to obtain high-quality clean reads by SOAPnoke, and mapped to the P. trichocarpa reference genome and reference genes using HISAT/Bowtie10