GSM3521332: 141114_SND405_A_L008_HLD-5; Venturia canescens; RNA-Seq
A total of 10 individual's heads were collected per experimental condition and pooled. Total ). RNA was extracted using Qiazol reagent and RNeasy MiniKit according to manufacturer protocol (Qiagen), with optional DNase treatment (Thermo Fisher Scientific). Polyadenylated RNA were enriched from 1 mg of high-quality total RNA with oligo-dT magnetic beads, fragmented and converted to cDNA (Illumina TruSeq Stranded mRNA Library Prep kit). After adding an A in 3' of cDNA, adapters were ligated, and fragments amplified with PCR to generate DNA colonies. Each library was labelled, multiplexed and pooled for sequencing on HiSeq 2500 Illumina sequencer (Fasteris, Switzerland), with paired-end protocol (2x125bp).
GEO Accession
GSM3521332