GBS of Helianthus annuus x Helianthus debilis subsp. debilis
We sequenced 1) two mapping populations each of 500 Helianthus                 annuus texanus; 2) individuals from natural population for Helianthus annuus, (4                 individuals/pop, 4 populations), Helianthus debilis (2-4 ind/pop, 3 pop), H. annuus                 texanus (2-4 ind/pop, 5 pop), Helianthus praecox (4 ind/pop, 2 pop). 3) 360 hybrid                 individuals descending from an original backcross (H. a. annuus x H. debilis F1 was                 crossed to H. a. annuus) mimicking the genetic origin of H. annuus texanus. These                 were planted and then allowed to evolve in 4locations at different generations                 between 2004 and 2011 (long-term experiment). Fourteen genotyping-by-sequencing                 libraries were constructed following the one enzyme protocol. Two libraries were                 built on whole genome amplified DNA samples. 100ng of genomic DNA was digested with                 the endonuclease PstI. Fragments were ligated to adaptors including the Illumina                 sequencing adaptor and size selection was performed to retain amplified fragments of                 length between 300-500bp. Pair-end sequencing was performed on an Illumina HiSeq                 2000 platform. This dataset is currently unpublished. This data was aimed to refine                 GWAS on 24 phenotypic traits measured in the field experiments (mapping populations)                 and provide first insight into allele fluctuations through time in the long-term                 experiment.