GSM3375059: CONTROL_Aged_2; Mus musculus; RNA-Seq
Sorted cells were directly dropped into lysis buffer and were used in the downstream RNA-seq library protocol. RNA-seq libraries were generated using Ovation® Single Cell RNA-Seq System (NuGEN Technologies Inc.). Cells were directly dropped into lysis buffer and reverse transcription was performed using oligo dT and selective primers to create first strand cDNA with randomly incorporated degradable nucleotide analog. Second strand cDNA priming was done using a forward adaptor with a random octamer attached to its 5' end. After performing end repair, the free end of the newly synthesized double stranded cDNA was ligated to the reverse adaptor and final Enrichment PCR was performed.
GEO Accession
GSM3375059