RNA-seq of A. thaliana RLCK VI_A2 mutants
From total RNA preparation polyA RNA were selected with NEBNext Poly(A) mRNA Magnetic Isolation Module then strand specific libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina and sequenced with an Illumina MiSeq using MiSeq Reagent kit V3-150 generating 2x75bp paired-end reads.