GSM4308814: RBM3_D24; Homo sapiens; RNA-Seq
Cells samples were lysed in Trizol reagent (Invitrogen, 10296010).ChIP-seq, cells were fixed, quenched, sonicated to ~250 bp in 1% SDS lysis buffer, and incubated with Protein A/G Dynabeads and 5 µg of the indicated antibody. 2% pre-cleared chromatin prior to primary antibody addition was kept as input DNA. After wash, treatment with RNAse A and Proteinase K, DNA was eluted and purified with Qiagen's PCR purification kit (28106). For RNASeq, library construction was performed using NEBNext Ultra II RNA Library Prep Kit (E7770) following manufacturer's instructions. For ChIPSeq, library construction was performed using KAPA Hyper Prep Kit (KK8500)  according to manufacturer's conditions. Libraries were submitted to PE150 high-throughput sequencing.
GEO Accession
GSM4308814