lake diversity planctomycete
Planctomycetes tag-encoded FLX amplicon pyrosequencing was performed as described previously (Bailey et al. 2009;Callaway et al. 2009;Dowd et al. 2008a;Dowd et al. 2008d;Dowd et al. 2008b;Dowd et al. 2008c;Leake et al. 2009;Suchodolski et al. 2009;Wolcott et al. 2009b;Wolcott et al. 2009a;Dowd et al. 2008d) at the Research and Testing Laboratory (Lubbock, TX.).  This planctomycetes tag-encoded FLX-Titanium amplicon pyrosequencing (TETAP) approach is based upon similar principles to bacterial TEFAP utilizing Roche FLX Titanium reagents and titanium procedures and a one-step PCR, mixture of Hot Start and HotStar high fidelity taq polymerases, and amplicons originating from the 40F region numbered in relation to E. coli rRNA.  The bTEFAP procedures were performed at the Research and Testing Laboratory (Lubbock, TX) based upon RTL protocols (www.researchandtesting.com).