16srRNA-seq of Shorea robusta
The DNA was isolated from samples using DNeasy PowerSoil kit (Qiagen, USA) as per the described protocol. The V3-V4 region of 16S rRNA was amplified usingspecific V3 Forward primer CCTACGGGNBGCASCAG and V4 Reverse primer GACTACNVGGGTATCTAATCC.The prepared library was sequenced in Illumina HiSeq 2500 with 2*250 cycles chemistry