RNA-Seq analysis of Clostridium difficile
Purified mRNA was extracted from three biological replicates of exponential, stationary, and sporulating C. difficile 630 erm      , and converted to cDNA as previously described. RNA sequencing was performed on a HiSeq 2500, yielding an average of 29.4 ( 4.5, sd) million 100-bp single-end reads per sample.