H3K4me2 ChIP-seq of human pancreatic NET sample PNET 7
Soluble chromatin (5 μg) was immunoprecipitated with 10 μg H3K4me2 (Millipore 07-030) antibody. ChIP-seq libraries were constructed by end repair of precipitated DNA, base extension with Klenow fragment and ligation of Y-form-sequencing adapters, followed by 14 cycles of PCR. Between enzymatic steps, fragments of the desired size were enriched using AMPure XP beads.
alignment_software
BWA 0.7.10