GSM3070586: E5g; Gallus gallus; RNA-Seq
Granulose cells were removed and frozen in RNAzol. the granulosa cells were isolated from 6 mm, F5 and F1 follicles on ice and dispersed in RNAzol reagent (Molecular Research Center, Cincinnati, OH, USA). The total RNAs were extracted according to the manufacturer's instructions and dissolved in diethylpyrocarbonate-treated H2O. RNA quantity and quality were assessed using a Nanodrop spectrophotometer and Agilent 2100 according to the manufacturer's instructions. Briefly, the mRNA from total RNA (at least 3 μg) were enriched by poly-A oligo attached magnetic beads with an integrity value＞8.0. Double-stranded complementary DNAs were synthesized with random hexamer primers and purified with AMPure XP beads. The inserts with expected size were concentrated and subjected for the analyses.
GEO Accession
GSM3070586