EUseq of U2OS cells expressing normal levels of cyclin E: 9 h DRB treatment and release for 240 min.
U2OS cells expressing normal levels of cyclin E were synchronized by mitotic shake-off and released into media containing HU and DRB for 9 h. Cells were then released from DRB for 240 min and EU added for 30 min before collecting the cells. The EU labeled RNA was then isolated and sequenced.