Ion Torrent Proton sequencing; Testis transcriptome alterations in zebrafish (Danio rerio) with reduced fertility due to developmental exposure to 17α-ethinylestradiol
Testis transcriptome alterations in zebrafish (Danio rerio) with reduced fertility due to developmental exposure to 17α-ethinylestradiol
RNA extraction Whole testis homogenized in TriReagent according to the manufacturer (0.8 ml/sample) (Sigma-Aldrich, Germany) EE2 exposure. Fish exposed to 0, 1.2 or 1.6 ng/L EE2 for 80 days starting 1 day after fertilization   RNA extraction Whole testis homogenized in TriReagent according to the manufacturer (0.8 ml/sample) (Sigma-Aldrich, Germany) Ion Whole transcriptome analysis kit on RiboZero (eukaryote) depleted total RNA
Experimental Factor: compound
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