GSM3374641: HDF-loading-IP; Homo sapiens; ChIP-Seq
Lysates were clarified from sonicated nuclei and ELK1-DNA complexes were isolated with Anti-ELK1 antibody[E277] - ChIP Grade (abcam,ab32106) DNA samples were end-repaired, A tailed, and adaptor ligated using TruSeq Nano DNA Sample Prep Kit (#FC-121-4002, Illumina), following the manufacturer's instructions. ~200-1500 bp fragments were size selected using AMPure XP beads. The final size of the library was confirmed by Agilent 2100 Bioanalyzer.
GEO Accession
GSM3374641