GSM2113637: Primary macrophages, untreated, replicate 2; Mus musculus; RNA-Seq
Total RNA was isolated using QIAgen RNAeasy kit from BMMΦ incubated for 18 h with or without 20 ng/ml of IL4 (3 biological replicates), or from WAT-derived CD45+F4/80+CD11b+CD11c+CD206- MΦ from HFD-fed WT and cKO mice For BMMΦ, RNA was polyA-enriched and converted into Illumina-compatible sequencing library with TrueSeq mRNA-Seq sample preparation kit (Illumina). For WAT-derived MΦ, SMART-Seq v3 Ultra Low Input RNA Kit (Clontech) followed by Nextera library prep kit were used to prepare Illumina-compatible sequencing libraries. Quality control of RNA and library was performed using BioAnalyser 2100 (Agilent).
GEO Accession
GSM2113637