GSM2043047: GEK26; Oryza sativa; miRNA-Seq
Total RNA was extracted from 100 mg of homogenized shoot tissue from ASR5_RNAi and NT plants using Trizol (Invitrogen, CA, USA) according to the manufacturer’s protocol. The quality of the extracted RNA was evaluated using 1 % agarose gel electrophoresis. Total RNA ([10 lg) was sent to Fasteris SA (Plan-les-Ouates, Switzerland) for processing and shotgun sequencing using an Illumina Hiseq 2000 (Illumina, CO). Four small RNA libraries were constructed: two from shoots of NT seedlings (Al treatment and control)  and another two from shoots of ASR5_RNAi seedlings (Al treatment and control). The libraries were constructed using the following successive steps: acrylamide gel purification of the RNA bands corresponding to 20–30 nt in size; ligation of the 30 and 50 adapters to the RNA in two separate subsequent steps, each followed by acrylamide gel purification; cDNA synthesis followed by acrylamide gel purification; and a final step of polymerase chain reaction (PCR) amplification to generate a cDNA colony template library for Illumina sequencing. All low-quality reads and adapter sequences were removed.
GEO Accession
GSM2043047