In-silico detection of polymorphic microsatellites using RAD-seq in the endangered Isis Tamarind, Alectryon ramiflorus.
DNA libraries were constructed using the Illumina TruSeq Nano DNA  library prep.  Briefly, 200 ng of DNA was sheared to ~550 bp by  ultra-focused sonication on the Covaris E220.Fragmented DNA underwent end repair and  size selection (large and small fragment removal) using SPRI beads followed by A-tailing (adenylation of the 3 ends) and Adapter ligation.  Adapter ligated fragments are amplified 8 cycles of PCR.      The final library is assessed by electrophoresis (D1000 TapeScree Assay, TapeStation 2200, Agilent) and quantified by qPCR (KAPA Biosystems).  The library was then sequenced on a MiSeq (Illumina, Inc.) instrument as per manufacturers instructions with 2x250 bp paired end reads.