RNA SEQUENCING of OVCAR- 3 cells
tome library for sequencing is made using Sense Total RNA-seqLibrary prep kit. This protocol maintains the strand specificity to allow themapping of the genes to the corresponding strand. The workflow involves depletionof ribosomal RNA, including the mitochondrial fraction, followed by the randomhybridization of Starter/Stopper heterodimers containing Illumina-compatible linkersequences to the RNA template. Reverse transcription starts from the Starter oligo andends at Stopper oligo where the cDNA is ligated. Then, the second strand is generatedfollowed by PCR amplification where P5 and P7 adapters are added which is requiredfor cluster generation.