Methane shale flowback fluids metagenome: Sample Sample_05012013
"We used Picogreen (Invitrogen) to quantitate genomic DNA samples. DNA was sheared using a Covaris and libraries were constructed with the Nugen Ovation Ultralow Library protocol  and were amplified for 11 cycles.  The amplified product was visualized on an Agilent DNA1000 chip or Caliper HiSens Bioanalyzer assay. Libraries were pooled at equimolar concentrations based on these results and size selected using a Sage PippinPrep 2% cassette.  The final library pool has an average insert size of 170bp.  it was quantified using a Kapa Biosystems qPCR library quantification kit, then sequenced on the Illumina HiSeq1000 in a 2x101 paired-end sequencing run using dedicated read indexing. The samples were demultiplexed with CASAVA 1.8.2."
experiment_number
3