testisF0H1
After exposure, fish were anesthetized in an ice bath. Testis tissues were dissected from randomly selected male fish (n = 6 each from normoxia and hypoxia). One pooled RNA sample was prepared from three testes per treatment group, and two replicates each were prepared for the normoxic control and hypoxic treatment. Total RNA from pooled tissue samples was extracted using the mirVanaTM miRNA isolation kit (Applied Biosystems). RNA quality was assessed using the Agilent 2100 Bioanalyzer system and samples with a RNA Integrity Number (RIN) greater than 9 were used for small RNA library construction.