WCS of Wetland: Troups Creek Australia: Sample 01072014S3S5
The V3-4 region of the bacterial rRNA gene was amplified. Sequencing data were demultiplexed using MiSeq Reporter V2.4.60 and quality trimmed and adapter filtered using Trimmomatic (Bolger et al. 2014). Reads were filtered to remove adapters and trimmed to remove any terminal stretches of bases at or below Q30. Reads shorter than 180b were discarded. Pre-cluster read pairs were trimmed and filtered to produce single reads using PEAR (Zhang et al. 2014). The assembled reads were analysed using the QIIME 1.8.0 open-reference OTU picking workflow with UCLUST for de novo OTU picking and the GreenGenes 13_8 release for the reference and for taxonomic identity assignment
alignment_software
QIIME