GSM3523542: Rbpj KO input replicate 1; Mus musculus; ChIP-Seq
The samples were washed twice in PBS and resuspended in commercial lysis buffer containing protease inhibitors from the MAGnify Chromatin Immunoprecipitation System kit (Thermo Scientific; 492024). The chromatin was sheared using the Covaris S2 sonicator (Covaris) at 4°C for 10 minutes with the following settings; duty cycle 5%, intensity: 2, cycles per burst: 200, cycle time: 60 seconds. The DNA library for sequencing was prepared using 20 ng of ChIP'ed DNA for the Kapa Hyper Prep kit with 16 cycles of PCR amplification. The quality of the final DNA library was confirmed on the Agilent Tapestation before the samples were submitted to sequencing on the HiSeq 4000 (Illumina).
GEO Accession
GSM3523542