GSM3674411: GbdR-2; Pseudomonas aeruginosa PAO1; RNA-Seq
To examine the effect of TFs (taking an example of RpoN) on the transcriptome, 2 ml of mid-log-phase (OD600 = 0.6) bacterial cultures (PAO1 and rpoN::Tc mutant strains) were collected by centrifugation (12,000 rpm, 4℃). An RNeasy minikit (Qiagen) was used TruSeq RNA sample prep kit protocol (Illumina)
GEO Accession
GSM3674411