Metagenomics:cocoa:fermentation:Time:54h
Aliquots of 250 mg of samples were collected for metagenomic DNA extraction using the PowerSoil DNA Isolation Kit (Mobio-Qiagen, Netherlands) following the manufacturers best practices. The recovered total DNA from samples was quantified by fluorometry using the Qubit dsDNA BR Assay Kit (Thermo Fischer Scientific) executed in Qubit device (Thermo Fisher Scientific) following manual guidelines.