RNA-Seq of dog-derived oral melanoma peripheral blood, post-therapy
Total RNA was isolated from peripheral blood using the PAXgene Blood RNA Kit, as per the manufacturer's instructions. Using or 1 µg of total RNA as input, mRNA was enriched using the NEB magnetic mRNA isolation kit and RNA-seq libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, MA) as per the manufacturer's specifications.