Tul7/2007 F. tularensis subspecies holarctica from Hungary
The library for this sample was prepared using sonication of 5 ?g of genomic DNA into an average fragment size of 350 bp, followed by sample preparation and cluster generation protocols for paired-end reads from Illumina. The library was quantified using SYBR-based qPCR and primers modified from the adaptor sequence. The library was then run in a single lanes of the flow cell to allow for sufficient coverage average. Read lengths were ca. 101 bp, thus we generated 32 G bp of sequence. Image analysis for base calling and alignments followed methods of Craig and colleagues.