Rhodnius prolixus Central Nervous System Transcriptome
CNSs were microdissected from adult male and female R. prolixus at different times post-blood meal (PBM): unfed, 1h PBM, 4h PBM and 24h PBM. Total RNA was isolated using Trizol. Four cDNA libraries were independently constructed with 1 µg of RNA from each group using Mint-2 Kit (Evrogen, Moscow, Russia), according to the manufacturer instructions for Illumina platform sequencing.