mitochondrial DNA resequence of kenaf: UG93A
5 g mitochondrial DNA was randomly sheared into fragments using a Covaris S220 with an insertion size of approximately 200-500 base pairs (bp) to construct a paired-end sequencing library. Fragmented DNA was combined with End Repair Mix, followed by the addition of A at the 3 end, and Illumina paired-end adaptor oligonucleotides were ligated to the sticky ends. Several rounds of PCR amplification were performed to enrich the adaptor-ligated DNA fragments. DNA Clusters of PCR colonies were resequenced on a HiSeq 2000 sequencing platform.