Illumina genome sequencing of BG2
Starting with 500 ng  1 g of gDNA, samples were sheared by sonication on the Covaris S2 System to a fragment size of approximately 165bp with the following conditions:  Duty Cycle 10%, Intensity 5%, Cycles/Burst 200, Time 60 seconds and Number of cycles 6.  Libraries were constructed according to the protocol provided in Illuminas TruSeq DNA Sample Prep Guide.  Assessment of the yield and size distribution of the amplified library was performed on the Agilent 2100 Bioanalyzer using the High Sensitivity Chip.  Libraries were pooled to a final concentration of 2nM. Pooled libraries were denatured with 0.1 NaOH to a DNA concentration of 20 pM. Denatured DNA was then diluted to a concentration of 10 pM with 10% PhiX spike in to load on an Illumina MiSeq for paired end sequencing run.