Streptococcus pneumoniae strain specific differences in sensitivity to daptomycin
Tn-seq library construction was performed as described (van Opijnen et al. 2009; van Opijnen and Camilli 2012; van Opijnen et al. 2014; van Opijnen et al. 2015). Six independent transposon libraries were constructed in wt TIGR4 and Taiwan 19F. Tn-seq experiments were performed in SDMM in the presence or absence of daptomycin. Tn-seq sample preparation, Illumina sequencing and fitness calculations were done as described (van Opijnen et al. 2006; van Opijnen et al. 2009; Mann et al. 2012; van Opijnen and Camilli 2012; van Opijnen and Camilli 2013; Carter et al. 2014; van Opijnen et al. 2014; van Opijnen et al. 2015).