GSM4314477: HEK293T_KD; Homo sapiens; MeDIP-Seq
DNA isolated from HEK293T and GM02639 cells were sonicated. The lysates were clarified and subjected to MeDIP by anti-5-methylcytosine antibody cocktail. MeDIP DNA was purified using PCR cleanup kit. Genomic DNA was isolated from GM02640 and GM02641 cells. The Ion Xpress(TM) Plus Fragment Library Kit (Cat. no. 4471269) was used for library preparation. All the steps were performed as per manufacturer's instructions for sequencing on Ion Torrent S5 platform without any barcoding.
GEO Accession
GSM4314477