Mitured transcriptome analysis of Danshen with solexa
De novo sequencing of Mitured transcriptome of Salvia miltiorhiza.After the total RNA samples extracted,Oligo (dT) magnetic beads  were used to rich eukaryotic mRNA .Then mRNA sample were broken into a short section of 200 to 700 bp. First cDNA chains and the second cDNA chains were synthesied with six random primers (random hexamers) in sequence.Purified with QiaQuick PCR purification kit and eluted with EB elution buffer,the end of fragements were repaired,  plused polyA sequence and connected the adapters.After fragment size selectionand using agarose gel electrophoresis and PCR amplification, sequencing built library were ready and sequenced with the Illumina GA IIx.