epithlium_2days_OA_3
The aim of this study was to determine the transcriptomic responses in the olfactory system (epithelium and lobe) for water-borne exposure to high CO2, as a proxy for ocean acidification, in European sea bass (Dicentrarchus labras). RNA was isolated from the olfactory epithelium and lobe of juvenile sea bass using RNeasy mini kit (QIAGEN), according to the manufacturers instructions. cDNA libraries were prepared using Illumina NextFlex Rapid RNA-Seq Kit and subsequent cluster generation using TruSeq PE Cluster Kit v3 - cBot  HS (Illumina, San Diego CA, USA). In total 16 and 24 samples were prepared for sequencing for the olfactory lobe and epithelium, respectively: 4-6 replicate samples from each of 4 treatment groups (400 ľatm (control) at 2 and 7 days exposure, and 950 ľatm at 2 and 7 days exposure). For the epithelium and lobe 8  and 16 samples per lane, respectively, were sequenced using an Illumina GAII platform (76bp paired-end).