GSM4263713: MPAT hiPSC-CM RNA; Homo sapiens; RNA-Seq
Total RNA was extracted using Trizol/chloroform extraction, using 273-400,000 cells per condition.  Total RNA was treated with DNAase I and recrystallized, followed by quality control analysis via Tapestation. Library preparation was conducted by BGI.  mRNA was purified via oligo(dT) purification. First strand cDNA was synthesized using random hexamers, followed by second strand synthesis.  End repair was conducted, followed by 3'-adenylation, adapter ligation, PCR amplification, and circularization.
GEO Accession
GSM4263713