metaviromes of horse gut
Ion proton shotgun sequencingPure metavirome DNA (approx. 1000 ng) was fragmentedto a mean size of 200300 bp using the Covaris S220System (Covaris, Woburn, Massachusetts, USA). Then,an Ion XpressPlus Fragment Library Kit (Life Tech-nologies) was employed to prepare a barcoded shotgunlibrary. Emulsion PCR was performed using the OneTouch system (Life Technologies). Beads were preparedusing the One Touch 2 and Template Kit v2, and se-quencing was performed using Ion Proton 200 Sequen-cing Kit v2 and the P1 Ion chip.