GSM3733453: aCPC14; Homo sapiens; RNA-Seq
Progenitors were harvested at passage 5-7, and Total RNA was extracted using the Qiagen RNeasy micro kit with DNase treatment, followed by a clean-up step (Qiagen RNeasy minElute columns). The RNA quality and quantity were verified with a NanoDrop 1000 Spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). RNA sequencing and processing was performed by VIB Nucleomics Core (www.nucleomics.be). Illumina TruSeq® Stranded mRNA Sample Prep Kit was used with 250 ng total RNA per sample. RNA libraries were prepared for sequencing using standard Illumina protocols (protocol 15031047Rev E,October 2013 ). Sequence-libraries of each sample were equimolarly pooled and sequenced on the Illumina NextSeq 500 (75 cycles High output kit, Single Reads) at the VIB Nucleomics core (www.nucleomics.be).
GEO Accession
GSM3733453