GSM2112870: TypeII KRasEtv wt SAM19521961; Mus musculus; RNA-Seq
Type II cells were isolated (EpCAM+;CD24-;CD45-;CD31-;Pdpn-) from the lung of the following mutant mice: (1) KRaswt/d12;RosaCreERT2 n=4 (2) KRaswt/d12; Etv5loxp/loxp;RosaCreERT2 n=4 (3) KRaswt/d12; Etv4KO/KO; Etv5loxp/loxp;RosaCreERT2 n=4 The cells were cultured in 100% matrigel with 100nM 4OHT in BEGM medium right after FACS for four days (medium change every other day), then in BEGM without 4OHT for another one day. The cells were then harvested and RNAs were extracted by RNeasy micro kit.
GEO Accession
GSM2112870