GSM4484446: MW_180_149_AA; Rattus norvegicus; RNA-Seq
Total RNA, including miRNA and other small RNA molecules, was isolated from a piece of the lung tissue using the miRNeasy Mini Kit (Qiagen, Inc.; Valencia, CA) following the procedure provided by the manufacturer, including optional DNase treatment with RNase-Free DNase (Qiagen, Inc.; Valencia, CA). One microgram total RNA/sample was used to create sequencing libraries using the IlluminaTruSeq Stranded mRNA Library Prep Kit   (Illumina, Inc., San Diego, CA) following the  manufacturer's protocol. The samples were PCR amplified (12 cycles) to enrich the DNA fragments containing the adapter molecules and to enhance the amount of DNA in the library using a Veriti™ 96 Well Thermal Cycler (Applied Biosystems; Foster City, CA).
GEO Accession
GSM4484446