GSM1236536: RA1_ChIPseq_tassel_HA; Zea mays; ChIP-Seq
ChIP preparation were performed as previously described (Bolduc et al. 2012; Morohashi et al. 2012).  Anti-GFP (ab290, Abcam) and anti-HA (H3663, Sigma) antibodies were used for immunoprecipitation of RA1-YFP and RA1-HA, respectively, and protein A agarose/salmon sperm DNA beads (16-157, Millipore) were used to precipitate antibody complexes. ChIP-seq library preparation were performed as previously described (Bolduc et al. 2012; Morohashi et al. 2012).  ChIP DNA was subjected to end repair and A-base addition, followed by ligation with Illumina adapters made by annealing Mltplx_short and Mltplx_long oligos.  Library amplification was performed by PCR using Phusion Hot Start II High-Fidelity DNA Polymerase (F549L, Thermo Scientific) with MltplxPCR1.0 and PCR2.0_indx1 to 7, followed by gel-size fractionation to obtain 200-500bp fragments.
GEO Accession
GSM1236536