Long amplicon SMRT sequencing of human: wildtype fibroblast DNA
Long amplicons (4-8  kb) were produced in a multiplexed PCR using PrimeStar GLX Polymerase (Takara  Bio) with DNA extracted from wildtype human fibroblast cells. A SMRTbell  library was constructed using the Template Preparation Kit 1.0 (Pacific  Biosciences) according to manufactuerer's instructions. Sequening was  performed on the Sequel system using 3.0 sequencing and binding chemistry and  a 10 hr movie time.