GSM1239652: WT rep1; Mus musculus; RNA-Seq
Total RNA was isolated from lung specimens by using an IKA-Ultra-Turrax® T8 homogenizer (IKA-Werke) and TRIzol® reagent (Invitrogen), according to the manufacturer's instructions. Genomic DNA contaminations were removed from total RNA by using DNA-free kit (Ambion) as per manufacturer's instructions. TruSeq RNA kit (Illumina) was used for library construction. Briefly, cleaved RNA fragments were copied into first strand cDNA using reverse transcriptase and random primers, followed by second strand cDNA synthesis using DNA Polymerase I and RNase H. These cDNA fragments went through end repair process, addition of a single 'A' base, and ligation of adapters. The products were then purified and enriched with PCR to create the final cDNA library. Multiplexed DNA libraries were normalized to 10 nM and pooled in equal volumes.
GEO Accession
GSM1239652