GSM4411111: R8200; Mus musculus; RNA-Seq
Total RNA was extracted using Arcturus PicoPure RNA Isolation kit (Applied Biosystems™ Thermo Fisher Scientific) according to manufacturer's protocol. All mouse RNAs were analyzed on Agilent Bioanalyser for quality assessment with median RNA Integrity Number of RIN 7.7. cDNA libraries were prepared using 2 ng of total RNA and 1ul of a 1:50,000 dilution of ERCC RNA Spike in Controls (Ambion® Thermo Fisher Scientific) using the SMARTSeq v2 protocol (Picelli et al., 2014) with the following modifications: 1. Addition of 20 µM TSO; 2. Use of 200 pg cDNA with 1/5 reaction of Illumina Nextera XT kit (Illumina, San Diego, CA, USA). The length distribution of the cDNA libraries was monitored using a DNA High Sensitivity Reagent Kit on the Perkin Elmer Labchip (Perkin Elmer, Waltham, MA, USA). All samples were subjected to an indexed paired-end sequencing run of 2x151 cycles on an Illumina HiSeq 4000 system (Illumina) (31 samples/lane).
GEO Accession
GSM4411111