GSM2559461: C18:1 CoA treatment replicate 3; Arabidopsis thaliana; RNA-Seq
Total RNA was extracted using the NucleoSpin® RNA Plant kit from Macherey-Nagel according to the manufacturer's  instructions. Subsequently, contaminating DNA was removed using the Turbo DNA-free kit from Ambion according to the manufacturer's instructions. RNA quality and integrity was assessed on a QIAxcel platform (Qiagen, Hilden, Germany). The TruSeq® Stranded mRNA sample preparation 96 rcx kit (Illumina™) following the low sample protocol according to Illumina™ guidelines was used to construct the library using 2.5 µg of total RNA. Adaptors were ligated in 12-plex formations and the libraries were quantified using PicoGreen® (Life Technologies™). Groups of 12 samples were pooled at equal concentrations to create the eight pools. The Kapa SYBR® FAST universal qPCR kit (Kapa Biosystems™) for Illumina™ sequencing was used to quantify the number of the amplifiable molecules in the pools and the Bioanalyzer® (Agilent Technologies™) to determine the average fragment size of our pools and optimization of the flow cell clustering. An illumina HiSeq 1500 sequencer was used in the high throughput mode using the TruSeq® SBS Kit in a 50-cycle pair-end run.
GEO Accession
GSM2559461