Histone H3K4me3
BCCAGSC MNase ChIP-seq Protocol
EXPERIMENT_TYPE
Histone H3K4me3
EXTRACTION_PROTOCOL
5U/ml of micrococcal nuclease (Sigma, USA) for 7 min at 37oC
EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR
NA
EXTRACTION_PROTOCOL_SONICATION_CYCLES
NA
CHIP_PROTOCOL
BCCAGSC MNase ChIP-seq Protocol
CHIP_PROTOCOL_CHROMATIN_AMOUNT
23.52 ug
CHIP_PROTOCOL_BEAD_TYPE
Protein A/G
CHIP_PROTOCOL_BEAD_AMOUNT
20 ul
CHIP_PROTOCOL_ANTIBODY_AMOUNT
2.5
CHIP_ANTIBODY
H3K4me3
CHIP_ANTIBODY_PROVIDER
Abcam
CHIP_ANTIBODY_CATALOG
ab8580
CHIP_ANTIBODY_LOT
376637