GSM4663151: Patient 2, Gel; Homo sapiens; RNA-Seq
After  4 days, mRNA was extracted using the Rneasy mini kit with DNase I digestion (Qiagen). RNA quality was ensured to be greater than 8 using a Bioanalyzer (Agilent). Ds-cDNA was consructed using Clontech SMARTer Ultra Low RNA kit for Illumina Sequencing. cDNA was fragmented using a Covaris E220 sonicator. cDNA was blunt ended,  an A base added to 3' ends, and then Illumina sequencing adapters ligated to the ends. Ligated fragments were amplified for 12 cycles and run on an Illumina NovaSeq reading 150 bases from both ends to a depth of 30 million reads per sample. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM4663151