NRCM from Rattus norvegicus Phenylephrine (50µM)_Isoproterenol (10µM) treatment 24h
Total RNA was isolated from NRCMs with the RNeasy Mini Kit (Qiagen). Starting material for library preparation was 500 ng total RNA. mRNA was isolated using magnetic beads (NEBNext® Poly(A) mRNA Magnetic Isolation Module, NEB). Library preparation was performed with NEBNext® UltraTM RNA Library Prep Kit for Illumina®. 13 PCR cycles were used for library enrichment. Agencourt® AMPure® XP Beads (Beckman Coulter) were used for purification and size selection.