18S amplicon sequencing of marine sediments affected by jellyfish decomposition 2cm replicate 3 Control
The 18S rRNA gene sequences were amplified using the 1391f-EukBr primer pair (Amaral-Zettler et al. 2009; Stoeck et al. 2010) in combination with CS1/CS2 tags (1391fc 5-ACACTGACGACATGGTTCTACA GTACACACCGCCCGTC, EukBr 5- TACGGTAGCAGAGACTTGGTCT TGATCCTTCTGCAGGTTCACCTAC), using the following PCR amplification protocol: initial denaturation at 94 C for 45 s, 30 cycles of denaturation (94 C for15 sec), annealing (60 C for 20 sec) and extension (72 C for 30 s). Library preparation from the PCR products and sequencing of 2x250 bp Illumina MiSeq reads was performed at HyLabs (Israel).