RNA-Seq of dog-derived oral melanoma tissue biopsy
Total RNA was isolated from the tissue biopsy using TRIzol then subsequently purified by the RNeasy Mini Kit, as per the manufacturer's instructions. Using or 0.5 µg of total RNA as input, mRNA was enriched using the NEB magnetic mRNA isolation kit and RNA-seq libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, MA) as per the manufacturer's specifications.