ChIP-seq of RNApol2 pulldown DNA from Quiescent  myoblast( C2C12 mouse)
20 ng of pull-down/input DNA was used to carry out sequencing library preparation using NEBNext¨ DNA Library Prep kit for Illumina¨ (New England Biolabs¨ , cat. No. E6000) along with NEBNext¨ Multiplex oligos for Illumina¨ (Index Primers set 1) (New England Biolabs¨, cat. No. E7335), as per manufacturerÕs protocol. The library thus prepared was quality checked on BioAnalyser and qPCR prior to carrying out paired-end sequencing.