RNA-Seq of Sus scrofa: 7-day old muscle tissue
Total RNAs were extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure, and the quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano Labchip Kit (Agilent, CA, USA) with RNA Integrity Number (RIN) value >7.0. Prior to constructing Illumina-indexed libraries, the total RNAs from each breed were pooled equally. Next, approximately 10 ?g of mixed total RNAs were used to remove ribosomal RNA according to the manufacturer's instructions of the Epicentre Ribo-Zero Gold Kit (Illumina, San Diego, USA). Following purification, the poly-A- and poly-A+ RNA fractions were fragmented into small pieces using Fragmentation Buffer under elevated temperature, and the cleaved RNA fragments were then reverse-transcribed to create the final cDNA libraries in accordance with the protocol of the mRNA-Seq sample preparation kit (Illumina, San Diego, USA).