mRNA.replicate3.timepoint.2
Total RNA was collected from the cell lysate using Trizol-chloroform extraction and elution with water. Genomic DNA was removed by DNase I digestion. We prepared barcoded libraries for sequencing using the Truseq Stranded mRNA HT library prep kit for mRNA. Libraries were pooled, denatured and diluted according to the NextSeq 500 protocol. Single-end sequencing of libraries was performed on the Illumina Nextseq 500 platform using high-output flowcells with 75 bp read lengths.