San Diego copepods subjected to hyperosmotic stress for 24 hr
Total RNA was extracted from the sample pool of individuals (> 0.5 g wet weight each) using Tri Reagent (Sigma-Aldrich) and mRNA was extracted from the total RNA using Poly(A) Purist Kit (Ambion). Complementary DNA (cDNA) library were prepared using Roche’s GS FLX Titanium Rapid Library Preparation Kit, and San Diego samples barcoded with multiplex identifier (MID) adaptors. Sequencing was performed by Roche Life Sciences.