CRISPR/Cas 9_Sm-omega-1_miSeq
The schistosome mansoni eggs were collected from S. mansoni infection (45-49 days) by collagenase digestion and Percoll sedimentation.  The omega-1 gene of these eggs were modified by Lentiviral CRISPR/Cas9 including single strand oligonucleotide donor (ssODN) containing 24 nt of 6 stop codon casette flanking with 50 bp homology arms on both 5' and 3' end to interrupt the omega-1 gene, exon 1.  The eggs were extracted for genomic DNA using DNAzol, then amplicon flanking double stand break expected mutations was amplified and ligated with Illunina index-adaptor by Qiagen-1 step amplicon library construction.