16s amplicon sequencing of fecal material from mice colonized with human microbiota corresponsing to sample 211254388
DNA was extracted by bead-beating followed by QiaQuick columns (Qiagen) and quantified by Qubit assay (Life Technologies). The V4 region of the 16S gene was amplified by PCR and paired-end 250bp reads sequenced on an Ilumina MiSeq.