Transcriptomic data of kidney from the spotted scat following different salinity acclimation
Scatophagus argus (21.3 ± 4.6g) were collected from Zhuhai (N22°09′16.82″, E113°21′41.50″), Guangdong Province, China and reared for 4 weeks in tanks containing 25‰ seawater (SW). The water temperature was maintained at 27 ± 1 °C. For long-term saline stress experiment, fish were assigned to 3 groups: hypo-saline acclimation (0‰ freshwater, FW) group (Fa; n = 15), hyper-saline acclimation (50‰ SW) group (Ha, n = 15) and one control group (25‰ SW, n = 15). Individuals in the Fa and Ha group were transferred directly from 25‰ SW to FW and 50‰ SW, respectively, where they were remained for 4 weeks. At the same time, control individuals were transferred from their original SW tank to a new SW tank with the same salinity. After 4 weeks, fish were anaesthetized with 50 mg/L MS-222 (Sigma-Aldrich, St. Louis, MO, USA), and kidney samples were collected from each group. Each set of 15 individuals was divided into 3 groups of 5 individuals, each group comprising 1 biological replicate.