Targeted sequencing of Costus
DNA was extracted from dry tissue using the NucleoSpin Plant Mini Kit II (Macherey-Nagel, Dren, Germany) according to the manufacturer's protocol, adding 5 uL proteinase K (20 mg/mL) to the digestion step and increasing the digestion incubation time to an hour. Library preparation and sequencing for 853 was performed by Rapid Genomics (Gainsville Florida)