Whole genome sequence of a passaged CyHV-3 isolate
Genomic DNA was extracted from cell culture supernatants or pellets, using the Nucleospin virus extraction kit (Macherey Nagel). DNA libraries were prepared with the Nextera XT DNA Library Prep kit (Illumina), using 1 ng of input DNA. Four replicates of sample KHV-T_P78 two replicates of sample KHV-T_P99 were sequenced on a single lane of a MiSeq instrument with MiSeq Reagent Kit v2, using 500 cycles (2 x 250 bases, paired-ends).