RNA-Seq of macaque fascicularis: full-term VPA-treated embryo brain
Total RNA was extracted from the PFC of VPA-exposed and age-matched control monkeys by Trizol reagent following the manufacturers instruction (Invitrogen). The RNA quality was examined by Bioanalyzer 2100 (Agilent) and the RIN (RNA integrity number) over 6.8 was qualified for cDNA library construction. First-strand cDNA was synthesized with a SuperScript III First Strand cDNA synthesis kit (Invitrogen) according to the manufacturer's protocol, followed by second strand cDNA synthesis using RNase H and DNA polymerase I (Invitrogen).