RNA-seq of Saccharomyces cerevisiae growing on yeast extract and methanol
30 mL of culture was centrifuged at 4 C 17,000 x g for 10 min, with pellets resuspended in RNA later (Sigma Aldrich R0901) and stored at -20 C prior to RNA extraction. Total RNA was extracted as previously described 40. Library preparation and sequencing was performed at the Ramaciotti Centre for Genomics using a TruSeq Stranded mRNA-seq preparation kit and NextSeq 500 2x75bp sequencing.