gut microbiome in rats fed NK603 and MON 810 GM maize for 6 months
Briefly, 50 ng DNA was used to generate amplicons that cover V3 and V4 hypervariable regions of bacteria and Archaea16S rDNA.  Indexed adapters were added to the ends of the 16S rDNA amplicons by limited cycle PCR. Sequencing libraries were validated using an Agilent TapeStation (Agilent Technologies, Palo Alto, CA, USA), and quantified by Qubit and real time PCR (Applied Biosystems, Carlsbad, CA, USA).