Metagenomic sequencing of cultures enriched with Mn(III):Lake Matano Sediments
Community DNA was processed using the Nextera XT DNA Sample Prep kit and sequenced using a paired-end Illumina MiSeq 600 kit. Barcoded sequences were de-multiplexed, trimmed (length cutoff 100 bp), and filtered to remove low quality reads (average Phred score <25) using Trim Galore! (http://www.bioinformatics.babraham.ac.uk/projects/trim_galore/).