Illumina sequencing of pooled DNA from fifty (50) herring fishes captured in Gamleby, Central Baltic Sea
Genomic DNA was isolated of muscle tissue from 400 fish collected from eight locations during 1978–1980; the samples had been stored at -20 *C until DNA was isolated. The DNA from 50 individuals per sampling location was pooled in equimolar concentrations. For each DNA pool, two libraries were constructed with 250- and 320-bp insert sizes at the SciLifeLab SNP&SEQ Technology Platform at the Uppsala Academy Hospital. These libraries were sequenced at 100 cycles using HiSeq-2000 sequencer (Illumina Inc., San Diego, U.S.A).