Aspergillus terreus transcriptome on glucose 48h
Culture mycelia were harvested after a 48h growth period on media with glucose as sole carbon source and immediately frozen in liquid nitrogen. Isolation of total RNA from frozen mycelia was performed according to Brasileiro & Carneiro (2015). RNA concentration and integrity were estimated with a NanoDrop 2000c spectrophotometer (Thermo Scientific, Waltham, MA, USA) and an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA). Messenger RNA isolation, cDNA library preparation, and Illumina RNAseq were performed by Eurofins MWG Operon (Alabama, USA). cDNA libraries were prepared using the TruSeq Sample RNA preparation kit v3 Kit according to the manufacturer's specifications and paired-end-sequenced (2x 100 base pairs) in two flowcell lanes on an Illumina Hiseq2500 sequencer (Illumina Inc, San Diego, CA, USA).