human saliva metagenome
gDNA extrated using commercial kit (Master Pure DNA Purification Kit, Epicentre, Madison, WI, USA and stored in -80C. Our library preparation protocol consisted of two main amplification steps, one to amplify and tag 16S rRNA variable region V4 (primers PE16S_V4_U515_F and PE16S_V4_E786R ), and a second to add final Illumina adapters.