miRNA-Seq of Arabidopsis
30ug total RNA was resolved on 15% urea-PAGE gel, and the 15-30nt regions were excised from the gel. sRNAs were recovered by soaking the smashed gel in 0.3M NaCl overnight, followed by precipitation with ethanol. Then total sRNAs and sRNAs extracted from AGO2 immunoprecipitation were used for sRNA construction with Illumina Truseq sRNA library preparation kit (Illumina).