Sinorhizobium phage vB_SmelM_phiM10
Two separate phiM10 DNA samples, each from a plaque-purified phage sample, were sheared with a Diagenode Bioruptor. Indexed libraries were constructed with an NEBNext Ultra DNA Library Prep kit for Illumina (NEB, Ipswich, MA) in accordance with the manufacturers instructions. For each library, 1 g of DNA was end repaired and ligated to NEBNext adapters. Fragments of an average size 736 bp for sample phiM10.2 and 1096 bp for sample phiM10.1 were isolated by electrophoresis on Bio-Rad Low Range Ultra Agarose and amplified for 8 to 10 cycles with NEB High-Fidelity 2X master mix and NEBNext multiplex oligonucleotides. Library size distribution was measured on an Agilent Bioanalyzer high-sensitivity chip, and quantity was determined with the KAPA Biosystems Library Quantification kit. Paired-end 300-base sequence reads were generated on an Illumina MiSeq with a 600-cycle MiSeq v3 Reagent kit. Genome assembly from MiSeq reads was performed with Lasergene SeqMan Pro v. 11.2.1.25 (DNAStar, Madison, WI). For both phiM10.2 and phiM10.1 the coverage was approximately 5000x. The assemblies predicted for phiM10.2 and phiM10.1 have only one mismatch out of 150041 bp. Both assemblies are circularly permuted.