RNA-Seq of mus  musculus: female whole blood_day 0_VCM_replicate_1
Mice were vaccinated with miltefosine-adjuvanted or non-adjuvanted influenza vaccine. 7 days after vaccination, mice were challenged with a lethal dose of H1N1 virus. Blood samples of mice were taken at day 0 right before vaccination, day 2 after vaccination, day 7 right before the virus challenge, and day 2 post-infection (day 9 after vaccination) using the RNAprotect Animal Blood Tube (QIAGEN). Total RNA was extracted from whole blood samples with the RNeasy Protect Animal Blood kit (QIAGEN) following the manufacturers instruction. All RNA samples were checked by Kapa qPCR for quantity and Agilent 4200 TapeStation for quality. Sequencing was performed on Illumina NovaSeq 6000 with Pair-end 150bp strategy.