C.alboguttata skeletal muscle transcriptome control frog 2
Poly(A)+ selection, mRNA library construction, cluster generation and sequencing was conducted by Macrogen Inc., Seoul, Korea. RNA samples were prepared for sequencing using a TruSeq RNA Sample Preparation Kit (Illumina) according to the manufacturer’s instructions. mRNA was purified from total RNA by way of poly (A)+ selection before samples were fragmented and reverse transcribed to cDNA using random hexamer priming. Following fragment end-repair, individual frog cDNA libraries were tagged by ligation of unique indexing adapters to cDNA ends in preparation for hybridisation onto a flow cell. PCR was then used to enrich for adapter-containing cDNAs followed by quality control analysis of each sample library and quantification of the DNA library templates. Prior to sequencing, DNA templates were bridge-amplified to produce clonal clusters on the surface of the flow cell. Sequencing was then carried out using an Illumina HiSeq 2000.