WGS of PC3 prostate cancer cell line
DNA was extracted using a QIAamp DNA mini kit (QIAGEN, VIC, Australia) from a low passage (passage four) of the PC3 prostate cell line cultivated from frozen stocks obtained directly from the ATCC. Sequencing was performed by Macrogen Inc. (Seoul, South Korea). Briefly, library preparation was performed using a TruSeq Nano DNA kit (Illumina) with a target insert size of 350bp. Paired-end libraries (150bp) were sequenced using a HiSeqX sequencer (Illumina). Base calls were converted into FASTQ files using bcl2fastq v2.15.0.