WGS of Timema cristinae: 10 pooled females
Genomic DNA isolated from a total of 10 individual females using Qiagen DNeasy Blood and Tissue kits (Qiagen, Inc.). All library construction and sequencing was carried out at BGI genomics institute, Hong Kong. Sequencing was accomplished on 7 lanes of an Illumina HiSeq 2000 machine with V3 reagents. Quality control included removing reads with greater than 5% Ns or with evidence of polyA regions, removing reads where 20% or more of the calls were considered low quality bases, removing adaptor polluted reads, removing reads with overlapping reads, and removing duplicated reads. This is a jumping library with 2000bp insert size