Lake Maggiore Cannobio
Sequence libraries of ?150 bp DNA paired-end fragments were prepared and sequenced using an Illumina NextSeq 500 V2. The generated sequences were demultiplexed using Illumina bcl2fastq 2.17.1.14 software and adaptors were removed. Reads containing three or more ambiguous nucleotides, or with quality score below 30 were removed before data analysis.