Protistan Microbial Observatory in the Cariaco Basin, Caribbean
we ran three independent PCR reactions for each of 24 samples, which were pooled and cleaned using the MinElute PCR purification kit (Qiagen, Valencia, CA, USA). PCR reactions for all samples were pooled into a single sample for pyrosequencing (Roche GS FLX Sequencing)  performed by MWG Biotech, Huntsville, Alabama.