MEF2 ChIP Exo July2013 HPC 130709_HS3A: Sample Input (HPC) 130709_HS3A
Abelson Murine Leukemia Virus (AMuLV)-transformed B cell line (pre-B) or LHX2-overexpresing hematopoietic progenitor cells (HPCs) from the black 6 mice were used in these experiments. MEF2C antibodies (Santa Cruz E-17 or Cell Signaling antibody D80C1)  or EBF1 antibody (Sigma antibody SAB2501166) were used in the immunoprecipitation. Pulled-down DNA was subsequently subjected to a series of enzymatic treatments: T4 DNA polymerase, P2 adaptor ligation (sequences adapted from Illumina small RNA preparation kit), phi29 polymerase, Lambda and RecJf exonucleases. Phenol-chloroform DNA purification was performed before the libraries were constructed (llumina), quality-controlled (2100Bioanalyzer, Agilent), and sequenced (HiSeq2000, Illumina).