ddRAD-seq
Around 200ng of gDNA was incubated for digestion with 4U EcoRI and 2U Mse1 (NEB) enzymes at 37¡ C for 3 hrs.11. Adapters were ligated and size selected on 2% agarose gel (400-500bp) and purified using Qiaquick gel extraction kit. The paired-end sequencing (2x101 nts) of ddRAD libraries were sequenced using HiSeq 1000.