HapMap HLA typing barcoded PCR proof-of-concept, 95 barcoded HapMap samples
95 HapMap human genomic DNA samples of known HLA type (a subset of the 270 HapMap samples) were amplified by PCR with six different exon-specific primer pairs.  For each primer pair, both the forward and reverse primers were tagged with molecular barcodes, such that 95 barcoded sets of primers consisting of 6 primer pairs each were used for 570 independent amplifications.  The 570 barcoded PCR products were then pooled prior to 454 library construction.