Transcriptome-based phylogeny of ticks of the genus Ixodes
Ticks (Ixodes holocyclus) were sampled in 2016, on the Houat island (France), by Dr Olivier Plantard. Ticks were found on captured European rabbits (Oryctolagus cuniculus). RNA extraction was performed in 2016, independently for two conditions (engorged adult females, unengorged adult females). An equimolar mix was used to produce a single final sample.  For all conditions, RNA was  extracted using a NucleoSpin RNA XS column (Macherey-Nagel, Dren, Germany) including a DNase and was stored in RNAsin (Promega, Madison, USA). The library was prepared using the Truseq Stranded kit (v4), and was sequenced on one lane of Illumina HiSeq 2500 along with other tick samples (different species of the genus Ixodes).