DNA-seq of Habronattus paratus: adult male legs and abdomen
DNA was extracted from the legs and abdomen of H. paratus using a QIAamp DNA Investigator Kit (Qiagen), assessed for integrity on a 21000 Bioanalyzer. Libraries were constructed with BIO-O NEXTflex Library Prep Kits (Bioo Scientific, Inc.) with insert sizes averaging 300 bp for DNA, and sequenced as 100bp paired-end reads on an Illumina HiSeq 2000 (Illumina, Inc.) at the Biodiversity Research Centre Next Generation Sequencing Facilities (University of British Columbia).