ZNS_MIX2
Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) and index codes were added to each sample for sequence attribution. Library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA) with the purpose of selecting cDNA fragments of 150~200 bp in length preferentially. Qualified cDNA was pre-processed with 3 l USER Enzyme (NEB, USA) prior to PCR. PCR was performed and then the products were purified (AMPure XP system). Quality of constructed library was assessed using Agilent Bioanalyzer 2100 system.