Wild type mouse retina RNA, replicate 2
Total RNA was extracted from the retina using Trizol. The contaminating DNA was removed by RNase free DNase digestion. The DNase was removed by chloroform extraction followed by ethanol precipitation of the RNA. Ribosomal RNA was removed from the samples containing 1µg of total RNA using Ribo-Zero™ Mouse rRNA Removal Kit (Epicentre). The Ilimina sequencing libraries were constructed from the rRNA depleted samples using the TruSeq RNA sample preparation kit v2 following the manufacturer's instructions. The libraries were quantified on Qubit fluorometer (Thermo Scientific) and the quality was assessed on Agilent Bioanalizer 2100.  The average fragment size was determined to be between 650 and 700 nucleotides.