WGA of dust metagenome: ISS Debris no-PMA
All nucleic acid samples were subjected to multiple displacement amplification (MDA) using the phi29-based Repli-g system (Qiagen). DNA libraries were prepared for sequencing using the Nextera DNA Library Preparation Kit (Illumina). Quality and fragment size were assessed on the Bioanalyzer 2100 (Agilent). Libraries were normalized to 2 nM, pooled, denatured, and diluted to 1.8 pM according to the manufacturer’s standard recommendations (Illumina). Sequencing was performed on the NextSeq 500 with the NextSeq Series High Output Kit v2 (Illumina), using 150 base pair, paired-end reads.