RNA-seq of Solanum lycopersicum roots
Sample proceeded from two plants root. The tissues were powdered in liquid nitrogen. Total RNAs were extracted from root samples (two independent replicates for each treatment) using a RNeasy Plant Mini Kit (Qiagen, La Jolla, CA) with addition of an on-column DNase I digestion, following the manufacturers protocol. RNA quantity and quality were determined with a Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific Inc., Wilmington, Delaware, USA) and sent to IGA Technology Services (Udine, Italy,www.igatechnology.com). cDNA libraries were quantified using an Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA) and sequenced with Illumina technology (Illumina, Inc.) generating single reads, 50 nt in length.