P12w_smallRNA_rep3
Small non-coding RNAs (~18-30 nt) were isolated from total RNA (same as used for mRNA sequencing) from each library construction by size fractionation on polyacrylamide gels, purified, 5' and 3' adapter-ligated, reverse transcribed, amplified by PCR and sequenced using HiSeq 2000 (Illumina) as according to the manufacturer's protocols to generate 50 bp single-end reads. All data for each small non-coding RNA library were filtered for low quality reads and/or for a poly-A tail, ensured to contain sequence data for both 5' and 3' adapters (i.e., were complete small RNA sequences), trimmed for adapter sequences and then filtered further based on length prior to subsequent analysis