RNA-Seq of Paris polyphylla var. yunnanensis: germinated seeds 60d, repeat 3
Fresh-harvested P. polyphylla var. yunnanensis seeds were rubbed to remove the outer testa using clean river sand. And then, these peeled seeds were soaked in 20   water for 24 hours, in which the water was refreshed for every two hours. Finally, these seeds were incubated in gibberellin solution of 200 ppm for one hour. The treated seeds were sowed in the seedling frame containing a 5 cm layer of the mixed media (peatmoss: leafmound: pearlite = 1:1:1) in its bottom. A 1 cm layer of mixed media was sprinkled in the top of seeds and the frames were set into the water and allowed to thoroughly soak. All seedling frames were placed in the thermostatic chamber (20   and 30-40% humidity) and the germination day was begun to count.