RNASeq of homo sapiens: adult male
Library preparation of Patient MSK-HN1 tumor mRNA was performed with the Illumina TruSeq Stranded mRNA Sample Preparation Kit. This involves oligo-dT-mediated purification of polyadenylated mRNA, mRNA fragmentation, cDNA synthesis with Superscript III and random primers (strand-specificity maintained by addition of dUTP and RNA-dependent first strand synthesis by addition of Actinomycin D), A-tailing, Illumina adapter ligation, and PCR amplification with 10 cycles. Libraries were subjected to Illumina HiSeq X sequencing, with paired end 125 bp read lengths.