RNA-seq of Habronattus aztecanus: adult male whole body
Total RNA was extracted from the whole specimen using a combination of TRIzol extraction (Life Technologies) and RNeasy Mini Kit (Qiagen) for RNA purification and DNAse digestion. Sample assessed for integrity on a 21000 Bioanalyzer. Libraries were constructed with BIO-O NEXTflex Library Prep Kits (Bioo Scientific, Inc.) with insert sizes averaging 220bp, and sequenced as 100bp paired-end reads on an Illumina HiSeq 2000 (Illumina, Inc.) at the Biodiversity Research Centre Next Generation Sequencing Facilities (University of British Columbia).