Interval mapping and development of molecular markers for red/green skin: oriental pear
Young leaves, specifically the first few leaves of the apex during the beginning of the vegetative period of each individual, along with those of the parents, were collected and immediately sent to the lab in liquid nitrogen and transferred to a -80 °C freezer. Genomic DNA was isolated using the CTAB method (Doyle 1987) and the library constructed for QTL-seq, InDel and SSR analysis.  The red-skinned pool was constructed from mixed equal amounts of DNA from 28 red-skinned pears, and equal amounts of DNA from a pool of 27 green-skinned pears were mixed to create the green-skinned pool. Libraries with 350-bp inserted fragments were constructed using the Truseq Nano DNA HT Sample Preparation Kit (Illumina USA) and sequenced using an Illumina HiSeqTM4000 instrument with 150-bp paired-end reads