DNA sequencing of Beeville Coccidioides posadasii strain
DNA yield was assessed using spectrophotometry on the NanoDrop ND-1000 system (Thermo Fisher Scientific). Sequencing libraries were prepared using the Kapa Biosystems kit (Kapa Biosystems, Woburn, MA) and 1g of DNA was used for input material. Libraries were multiplexed using 8-bp indexes and quantified using quantitative PCR (qPCR) in a 7900HT system (Life Technologies Corporation, Carlsbad, CA) using a Kapa library quantification kit (catalog number kk4835; Kapa Biosystems, Woburn, MA)