WGS for COLO320HSR
Genomic DNA was extracted from cells using Qiagen kits. Sequencing libraries were prepared using TruSeq adapters (Illumina) and the KAPA HyperPlus kit, according to manufacturers instructions (Kapa Biosystems). Briefly, 250 ng of DNA was used as input and enzyme-fragmented for 12 minutes to obtain mode fragment lengths of 350 bp. KAPA Pure Beads were used for double-sided size selection of 250-450 bp. DNA libraries were pooled and paired-end DNA sequencing (150 cycles) was performed.