Illumina HiSeq 2000 paired end sequencing
Pool-Seq - shotgun sequencing of a DNA pool of individuals sampled from the same population. The pool size is large compared to the depth of coverage in order to ensure the reads represent a random sample of individuals from the pool at any given site. Thus, variation in base calls among the reads (within and between populations) represent SNPs and allele frequency data can be obtained from across the genome.