Genotyping-by-sequencing (GBS) of diploid Rosa "J14-3xSC-D3608"
Genomic DNA from 6 parents and 468 individuals of twelve populations was digested using the restriction enzyme NgoMIV. These samples were barcoded and pooled in groups of 66 and sequenced on an Illumina HiSeq 2500 using a 126bp single-end sequencing strategy. Sequence data was un-multiplexed, and the barcodes were removed using custom perl scripts. The resulting trimmed reads for each sample are provided as fastq files.