PM4_NAP
Total RNA was first extracted using RNeasy mini kit (Qiagen). Tru-seq (non-stranded and PolyA selected) sample preparation kits (Illumina) were then used to purify poly-A transcripts and generate libraries with multiplexed barcode adaptors. Single-end libraries were multiplexed, pooled, and sequenced using the Single Read Clustering with 100 cycles for 3 lanes and 50 cycles for 6 lanes on an Illumina HiSeq 4000. Sequencing was performed by the Weill Cornell Medical College Genomics Resources Core Facility.