20 SMRT cells of Drosophila yakuba NY73PB PacBio RS II reads
High Molecular Weight DNA extraction with QIAgen Blood and Tissue kit (Genomic-tip 100/G), sheared with 24 gauge blunt-end needle (Chakraborty et al. 2015 BiorXiv), verified length distribution by FIGE, submitted 40 ug for 2 library preparations (i.e. 20 ug per prep, 10 SMRT cells per prep) using BluePippin 15-50kb size selection, standard PacBio 20kb library preparation without Covaris G-tube step, followed by final BluePippin 15-50kb size selection of SMRTbell templates. Sequenced 20 SMRT cells, totalling ~13.5 Gb.  Each library prep resulted in 2 runs of the PacBio RS II sequencer: a Titration run of 2 SMRT cells (to determine optimal loading concentration), and a Main run of 8 SMRT cells at the optimal loading concentration determined by the titration run.  The second titration run (all SMRT cells starting with R063) turned out not to be a titration, but just validation that 400 pM loading concentration was still producing high yield and quality.  Thus, only R053-H01.1 was run at 300 pM loading concentration, and all 19 other SMRT cells were run at 400 pM.