Trichoderma: Sample Hebe_strictissima
"Total community DNA was amplified with the set of Trichoderma-specific primers, (ITS1Trf/ITS4Trr Meincke et al., 2009) in the nested PCR approach using Taq-Go Ready-to-use PCR Mix (MP Biomedicals). Duplicate PCR products from all templates (eight per plant) were purified with Wizard SV Gel and PCR Clean-Up System (Promega)."