Lupinus angustifolius cv. Tanjil gDNA 40 kb insert mate-paired reads
-     Illumina adapter (TruSeq v3) and <Q30 end-trimming via CutAdapt v1.1 o     Minimum read length after trimming 25 bp o     match length 5 bp o     3 iterations -     Reads with ≥5 bp poly N discarded -     Overlapping pairs merged via FLASH v1.2.2 discarded as paired-end contaminants (shadow filter step 1) -     Mate-pairs aligned to preliminary PE-only de novo assembly with bowtie2 as FR reads with insert size ranging from 0 to 1000 bp.  Concordantly aligned pairs were discarded as paired-end contaminants (shadow filter step 2)