Digital gene expression libraries of larvae, pupae and adults of Tomicus yunnanensi.
Total RNA was extracted from 20 larvae, 20 pupae and 20 adult beetles with TRIzol Reagent (Ambion) following the manufacturer's instructions. RNA concentration and quality were measured by NanoDrop™ 1000 spectrophotometer (Thermo Scientific). Genomic DNA was digested with the treatment of DNase I (Fermentas). The cDNA library was prepared using Illumina's kit according to manufacturer's recommendations.