Sinorhizobium phage phiM6.1
Two separate phiM6 DNA samples, each from a plaque-purified phage sample, were sheared with a Diagenode Bioruptor. Indexed libraries were constructed with an NEBNext Ultra DNA Library Prep kit for Illumina (NEB, Ipswich, MA) in accordance with the manufacturers instructions. For each library, 1 microgram of DNA was end repaired and ligated to NEBNext adapters. Fragments of an average size 1142 bp for sample phiM6.1 and 940 bp for sample phiM6.2 were isolated by electrophoresis on Bio-Rad Low Range Ultra Agarose and amplified for 8 to 10 cycles with NEB High-Fidelity 2X master mix and NEBNext multiplex oligonucleotides. Library size distribution was measured on an Agilent Bioanalyzer high-sensitivity chip, and quantity was determined with the KAPA Biosystems Library Quantification kit. Paired-end 300-base sequence reads were generated on an Illumina MiSeq with a 600-cycle MiSeq v3 Reagent kit.