RNA-Seq data of human adult colon tissues (1)
2 different fetal normal tissues and 6 different adult normal tissues were purchased from different sources (Agilent, Biochain and OriGene). The qualities of these total RNA were tested using the Agilent Bioanalyser 2100 Eukaryote Total RNA Nano Series II. Only total RNAs with a RIN score of more than 7 were used for RNA-Seq library construction. Ribosomal RNA (rRNA) was removed from total RNA using the RiboMinus™ Eukaryote Kit for RNA-Seq from Ambion. The ribosomal RNA depleted RNA fraction is termed the RiboMinus™ RNA fraction and is enriched in polyadenylated (polyA) mRNA, non-polyadenylated RNA, pre-processed RNA, tRNA, and may also contain regulatory RNA molecules such as microRNA (miRNA) and short interfering RNA (siRNA), snRNA, and other RNA transcripts of yet unknown function. Ambion RiboMinus rRNA depletion was performed as described in the manufacturer’s protocol (Pub. Part no.: 100004590, Rev. date 2 December 2011) following the standard protocol. TruSeq RNA Sample Preparation was performed on the RiboMinus™ RNA fraction as described in the manufacturer’s protocol (Pub. Part no.: 15026495 Rev. F March 2014) following the low sample protocol. The libraries were sequenced on Illumina’s HiSeq 2000 instrument following standard protocol.