16S rRNA-seq of human gut metagenome: stool sample
DNA was extracted from stool samples, and microbial 16S region was amplified by PCR using primers specific to the V3 forward primer (TCGTCGGCAGCGTCAGATGTG-TATAAGAGACAGCCTACGGG-NGGCWGCAG) and V4 reverse primer (GTCTCGTGGGCTCGGAGATGTG-TATAAGAGACAGGACTACHVGGG-TATCTAATCC).