RNA-Seq of Plasmopara halstedii 710: 1dpi H. annuus resistant root
Two day-old seedlings of Helianthus annuus line TSRM were root inoculated by Plasmopara halstedii, pathotype 710, with 100 000 spores and sporangia (between 5 and 10 um diameter) /mL  for 4 hours (Mouzeyar et al., 1993) and grown afterwards in oxygenated liquid growth medium under hydroponic conditions, at 18C, 80% humidity and a photoperiod of 16h of light. Infected root tissues were collected at 24h post inoculation, snap-frozen with liquid nitrogen and conserved at -80 C.Total RNA was extracted with the Nucleospin RNA II kit from Macherey- Nagel, using RAP buffer for cell lysis. RNA quality was verified using an Agilent Bioanalyzer 2100 LabChip.mRNA were selected using poly-T beads and libraries were prepared at the GeT-PlaGe facility (Toulouse, France) according to Illumina protocols using the Illumina TruSeq Stranded mRNA sample prep kit.