Heliconius erato emma, Day 1, hindwing cDNA
cDNA was end repaired using an adjusted Illumina protocol involving T4 DNA polymerase and polynucleotide kinase and a Klenow DNA polymerase from New England Biosystems. An A was added to this purified product using Klenow 3' to 5' exo- and dATPs. Adaptors were ligated to the resulting purified product using Quick DNA ligase from New England Biosystems and 1 microliter Illumina adaptor oligo mixes at 1:9 microliter dilution. The resulting purified product was run an a gel and bands from 150-275bp were cut. The purified product from this was PCR enriched for 18 cycles using a Phusion polymerase and a final purification was performed. All purifications were performed with Qiagen kits. For more details on the protocol see documents accompanying the manuscript.