ONT sequencing of Bordetella pertussis UK39
gDNA extracted using genElute kit (Sigma), sheared to 20 kb using g-tubes (Covaris), library prep with ONT's 1D ligation (SQK-LSK108) and native barcoding (EXP-NBD103). 10 barcoded samples were sequenced together. Basecalling with Albacore (v2.1.3), demultiplexing with Albacore and Porechop (v0.2.1)