Bombus huntii-Adult worker02
A cDNA library was prepared with mRNA or cRNA using the cDNA rapid  library preparation kit (Roche, Branford, CT) according to the manufacturer’s instructions with minor changes. Briefly, mRNA or cRNA was fragmentated,  first and second-strand cDNA was synthesized. The double stranded cDNA was obtained by using AMPure beads and  will further purified by gel purification (500-800 bp). The fragment end repair and adaptor ligation was prepared and the quality of cDNA library was checked on an Agilent Bioanalyzer. cDNA libraries were then amplified by running emulsion-PCR and sequences were performed in a Roche GS-FLX system.