RNA-Seq of Arabidopsis thaliana:rosette leaves of dcl4rdr6 mutant
Arabidopsis plants were grown in soil at 22¢XC under 70-125 £gE-2 sec-1 irradiance (16 h light and 8 h dark).Total RNA extracted from 21-day-old rosette leaves with PureLink Plant RNA Reagent (Thermo Fisher Scientific) was used in library construction for RNA-seq profiling. The RNA-seq libraries were prepared using the Illumina TruSeq RNA Library Preparation Kit v2 (http://www.illumina.com/).