high throughput sequencing of microorganisms: acid mine drainage
The SRA file SRR4254526 contains reads from 55 AMD tailing water samples. After downloading the file and decompression, the users can process the read data with the Mothur software package including data filtering, quality control, assembling of paired-end reads, and removal of primers, chimeras, and low-confidence singletons. Quality sequences were subsequently assigned to samples according to their unique 12-bp barcode provided in Table S1 in the paper entitled "Fine-scale Spatial Heterogeneity of Microbial Community Compositions in an Acid Mine Drainage", and then binned into phylotypes using UPARSE platform at the 97% similarity level for further analysis.