Target-sequencing of gastric biopsy
Genomic DNA (100ng) was used for capture-based enrichment of 781.132 bp of a customized gene panel including 98 of the most frequently mutated genes in gastric cancer. Libraries were prepared following the manufacturers instructions (SeqCap EZ Library SR Users Guide Version 5.1 - NimbleGen, Roche), and were sequenced in the NextSeq 500 platform (Illumina), using paired-end reads (2x75bp).