Sequencing of NA12878 on Ion Proton
In total, 100 ng of DNA (100ng/l) used as a starting material. Targets were amplified in Ion AmpliSeq RDY plate using primer sets as provided by Life Technologies (96 welled plate, processing of 8 samples on one plate). Next, primer sequences partially digested from amplicons followed by ligation of barcode adapters, and purification using AMpure beads to get the unamplified library. The unamplified library was quantified using quantitative real-time PCR before template amplification on ion spheres.