Ribosome profiling of HEK293 cells treated with VER
Ribosomes were isolated from cell lysates using sucrose gradient and ultracentrifugation. Ribosome fractions were combined and digested by RNase I. Then, RNAs were extracted and ribosome protected mRNA fragments were size selected and purified. Next, framents were added with Poly-(A) tails and converted to cDNAs by reverse  transcription. Afterwards, cDNAs were circulated and re-linealized. Finally, cDNAs were PCR amplified and library fragments were isolated.