RNA-seq of WT-3  mitochondria: tomato fruit
The fragmented RNA acted as a template and 6 bp random primers was added (random hexamers) to synthesis first cDNA chain. Then the buffer, dNTPs (dTTP dUTP to replace of dNTP), DNA polymerase I and RNase H was added to synthesis the second chain of cDNA. The synthetic double strand cDNA was purified and repaired, then A base and sequencing adapter was added. The second strand of cDNA containing U was degraded by USER enzyme and double strand DNA was enriched by PCR. Finally, AMPure XP beads were used to purify PCR products to obtain the final strand -specific library