Transcriptome Analysis in Scutellaria baicalensis Georgi: Stem
Three-year-old S. baicalensis plants were grown in the experimental field of Beijing University of Chinese Medicine(Beijing,China)during the natural growing seasons. Leaves, stems, flowers and main roots were sampled separately at the bloom stage on July 8th, 2013 and frozen immediately in liquid nitrogen until RNA extraction. Total RNAs were isolated using Trizol according to the manufacturer's protocols and then treated with RNase-Free DNase to remove residual genomic DNA contamination. The quality and quantity of RNA for RNA-seq sequencing libraries was assessed using the Agilent Technologies 2100 Bioanalyzer with an RNA integrity number (RIN) of more than 7.