DNA-Seq: microbes mineral matrix sample cold water spring
Four ~2g samples of Crystal Geyser Site 5 material preserved in Life Soil Preservation solution (MoBio) at -20oC were thawed on ice, spun at 2500g 5 min at RT and preservation solution removed, total RNA and DNA was isolated using PowerSoil RNA and DNA Accessory kit (MoBio) following manufacturers instructions. DNA recovered from the 4 samples was combined. Concentration and purity of DNA  was assessed using a nanodrop (Thermo Scientific). DNA library was prepared by the New York Genome Center using a TruSeqDNA PCR-free kit (Illumina).