Different  feeding strategies on Indian cattle and its effect on rumen methanon  diversity
Amplicon libraries  were prepared using the Nextera XT Index Kit (Illumna Inc, USA). Archaea  specific primers (forward: ACGGGGYGCAGCAGGCGCGA; reverse:  GGACTACVSGGTATCTAAT) were used for the amplification of 16s rDNA. Amplicon  libraries were purified by AMPureXP beads and quantified using Qubit  fluorometer. The amplified libraries were analyzed on 4200 Tape Station  system (Agilent Technologies) using D1000 Screen Tape.