cMyc capture B-Cells 53BP1-/- AIDrv (Sample B)
Linkers were removed by AscI digestion. Fragments were blunted by End-It DNA Repair Kit (Epicentre), purified, adenosine-tailed and ligated to Illumina paired-end adapters. Higher molecular weight products were isolated by agarose gel electrophoresis and adapter-ligated fragments were enriched by 25 cycles of PCR with Illumina primers PE1.0 and PE2.0. Forward and reverse libraries for the same sample were mixed in equimolar ratios and sequenced by paired end deep sequencing on an Illumina HiSeq2000.