Jack pine 454 sequence data from cDNA libraries
Total RNA was extracted by using a cetyl trimethylammonium bromide (CTAB) extraction protocol based on Chang et al. (1993) and described in detail by Pavy et al. (2008). Total RNA from drought trees, wound day 8, wound day 4, wound day 2, wound day 1 and control trees were pooled prior to cDNA synthesis. Double stranded cDNA was synthesized by Evrogen using the SMART approach (Zhu et al. 2001). Normalization included denaturation/reassociation and treatment by duplex-specific nuclease (Zhulidov et al. 2004). cDNA was sequenced using a 454 GS FLX Titanium platform at the McGill University and Genome Quebec Innovation Centre.