Continuous culture assay of 23S rRNA mutants: t=24
a mixture of SQZ10 E. coli harboring PLK35 plasmids with various mutations in the 23S rRNA gene were grown in a turbidostat clamped at OD 0.1. Sample was collected at the indicated time point. Plasmid was extracted by performing a Miniprep and sequencing libraries were prepared using PCR.