RNA-seq of C. elegans: ash-2 RNAi germline
RNA-seq on micro-dissected full germlines from N2 hermaphrodite C. elegans treated with ash-2 RNAi, and collected at initation of reproduction (day 2.5 of life). The poly-A selected RNA-seq library was generated on 20 full germlines using the Clontech SMART-Seq v4 Ultra Low Input RNA Kit for cDNA synthesis (634889, Clontech) and the Illumina Nextera XT DNA Library Prep Kit for library construction (FC-131-1024, Illumina).