Sequence capture of Rhinolophus sinicus
DNA libraries with insert fragments of ~300 bp were constructed for each sample individually using the NEB Next Ultra DNA Library Prep kit (New England Biosciences). Baits were then hybridized with libraries according to the standard protocol for the MYbaits kit (Arbor Biosciences). After hybridization, the baited libraries were recovered with Streptavidin C1 magnetic beads and were washed in order to remove the off-target sequences. Finally, post-hybridization samples were indexed during PCR amplification using the Agilent SureSelectXT 96 index primers.