18S rRNA amplicons  of Sceloporus grammicus fece's. Adult male.
The 18S rRNA gene was amplified with the primers nu-SSU-0817 and nu-SSU-1196. Amplification reactions were done in quadruplicate, pooled and purified using QIAquick PCR purification kit according to manufacturers instructions. The quantification of the PCR products was done using NanoDrop 3300 fluorospectrometer with PicoGreen dsDNA assay and combined in equimolar quantities for sequencing by Macrogen Inc.