Whole genome sequence of a wild-type CyHV-3 isolate
Total genomic DNA was extracted from koi gills with the Wizard Genomic DNA purification kit (Promega) and specifically enriched with CyHV-3 sequences through hybridization to a set of nearly 2 million overlapping probes designed to cover the entire length of CYHV-3 genome, using KHV-J sequence (GenBank accession number AP008984) as reference. Library was prepared with the TruSeq kit (Illumina) and DNA sequenced on a HiSeq2000 platform (Illumina) together with 7 other CyHV-3 specimens in a paired-end (2x100 bases) format.