16S rRNA-seq water samples: Lake Cajititlan
DNA from the filters (20-25 m and 0.45 m) was extract using FastDNA Spin Kit for Soil (MP Biomedicals, OH, USA) and amplified using the V3-V4 hypervariable regions of the 16S rRNA gene. The DNA products were purified using magnetic beads (AMPure XP - Beckman Coulter) and Indexed with Nextera XT kit (Illumina). The 96 samples were pooled at a concentration of 8 pM and loaded with 10% Phix control.