Amplicon sequencing of coral reef seawater
Seawater was filtered onto 0.22 um SUPOR DNA filters using                 peristaltic pumping and DNA was extracted from the filters using a variation of the                 sucrose-lysis DNA extraction method. The universal v4 primers 515F and 806R were                 used to amplify and barcode the v4 hypervariable region on the 16S rRNA gene of                 bacteria and archaea. Barcoded PCR products were sequenced using Illumina MiSeq                 sequencing.