RNA-seq of KO mouse ID8153 uterine nodule
otal RNAs from each mouse (pooled of three uterine nodules from same mouse) were isolated using TRIzol and cleaned up using RNeasy Mini Kit (Qiagen), and RNAs quality were determined using a 2100 Bioanalyzer (agilent) to make sure RIN number is above 8. RNA libraries were prepared using the TruSeq Stranded mRNA library Preparation Kit (Illumina). Briefly, RNAs were reverse transcripted to cDNAs and adapters with uniq barcode were ligated to RNA libraries from mouse. The libraries were then pooled in eauimolar amounts. The pool was then loaded on two lanes of an Illumina HiSeq 2500 Rapid Run flow cell (v1) and sequenced using TruSeq Rapid SBS Reagents in a 1x50bp single read format. Base calling was done by Illumina Real Time Analysis (RTA) v1.18.64 and output of RTA demultiplexed and converted to FastQ format with Illumina Bcl2fastq v1.8.4.