spsequel-AM
After enriched by Oligo (dT) magnetic beads, the mRNA is synthesized to cDNA using Clontech SMARTer PCR cDNA Synthesis Kit . Double-stranded cDNA was generated by PCR amplification and size selection (0.5k-6k). Large scale PCR was performed for SMRTbell library construction. SMRTbell templates were then generated following SMRTbell Template Prep Kit, annealed to the sequencing primer and sequenced on the PacBio Sequel platform using V2.1 chemistry with 10 h movies.