10X sequencing of Hirschfeldia incana: flower
The 10X Genomics library was made using the 10X Genomics ChromiumTM Genome v1 kit.The NEBNext Ultra DNA Library Prep kit for Illumina (cat# NEB #E7370S/L) was used to process the sample. Fragmentation of the DNA using the Biorupor Pico (Diagenode), ligation of sequencing adapters, and PCR amplification of the resulting product was performed according to the procedure described in the NEBNextUltra DNA Library Prep kit for Illumina Instruction Manual.