exome +UTR seq of human esophagus: purified Barrett's epithelium from patient 431
Pre-capture sequencing libraries prepared from 1ug intact genomic DNA, fragmented to 300 bp and prepared using KAPA DNA Library Preparation and HiFi PCR Kits on a PerkinElmer Sciclone NGSx Workstation. Individually indexed KAPA libraries were hybridized in solution to the NimbleGen SeqCap EZ Human Exome + UTR library set and sequenced on an Illumina HiSeq 2500 in high-output mode using v4 reagents and a paired end 100 base read depth sequencing strategy.  The SeqCap EZ Human Exome + UTR probes enrich for 64 Mb of coding exons (from SeqCap EZ Human Exome Probes v3.0) and miRNA regions, plus 32 Mb of  5'- and 3'-untranslated regions (UTRs) obtained from NCBI Reference Sequence (RefSeq) refGene table from UCSC GRCh37/hg19 March 2012 and Ensembl (GRCh37 v64).