Zea mays ssp. parviglumis Genome sequencing and assembly
Tissue samples were harvested from inbred line of Zea mays ssp. parviglumis, TIL11 (Selfed 9 generations, original collection from Tzitzio, Michoacan State, Mexico).  It was assayed on an Illumina 55K SNP chip, to estimate residual heterozygosity (0.0023 ). Genomic DNA was isolated from leaflet tissue, 3 Illumina libraries were generated, and the following sequencing was performed for each line: 1) one lane of 100bp paired-end HiSeq sequencing (insert size of 180bp); 2) one lane of 100bp mate-pair HiSeq sequencing (insert size of 2000bp), and one lane of  150bp mate-pair HiSeq sequencing for insert size of 6000bp