RNA-Seq for differentiating mouse b-cells: Sample clp RNA knockout
Common lymphoid progenitor cells (CLPs) from WT or Mx1-Cre Mef2c-KO mice (age 6-12 weeks) were sorted via fluorescent-activated cell sorting. RNA was extracted with Trizol and linear polyacrylamide and poly-A selected. Libraries were constructed according to manufacturer’s instructions (Illumina), quality-controlled (2100Bioanalyzer, Agilent), and sequenced (HiSeq2000, Illumina).