ATAC-seq of resting mouse B cell: adult spleen
Splenic B cells in the presence or absence of anti-IgM (1 hour, 10 μg/ml) were used for preparation of ATAC-seq library. Using 50000 cells of control and anti-IgM-stimulated splenic B cells, the ATAC-seq libraries were constructed by Nextera DNA Library Preparation Kit (Illumina) following the previous research (Buenrostro et al., Nature Methods, 2013). To remove primer-dimer, the prepared libraries were purified using the same volume of AMPure XP beads once. 101 bp pair end sequencing was performed by HiSeq2500 (Illumina).