Archaeal feces sequencing of prediabetic subjects with obesity before antibiotic therapy - methane non-eradicated subject
Archaeal 16S rRNA gene amplicons were generated using high-fidelity Platinum SuperFi Polymerase and the primers ARC344F (5 - TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGACGGGGYGCAGCAGGCGCGA  3) and Arch806R (5 - GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGGACTACVSGGGTATCTAAT  3). Amplicon quality was assessed using the DNA 1000 assay on an Agilent 2100 Bioanalyzer. Amplicons were then uniquely indexed in a PCR reaction using Nextera XT Index Kit v2 Sets A-D and KAPA HiFi HotStart Ready Mix. Library enrichment was performed using 8 cycles of PCR.