RNA Editing in Heart tissue of DO Mice
Total RNA from heart tissue was isolated by the Genome Technologies core facility at The Jackson Laboratory using the miRNeasy Mini kit (Qiagen), according to manufacturers protocols. Poly(A) RNA-seq libraries were constructed at the New York Genome Center using the TruSeq Stranded mRNA Library Prep Kit (Illumina). Libraries were pooled and sequenced 100 bp single-end on the HiSeq 2500 (Illumina) using TruSeq SBS Kit v4 reagents (Illumina) at the New York Genome Center.