RNA-seq of mus musculus visual cortex, 4TU-tagged cell types enriched in layer 2/3, streptavidin purified from a Sepw1-cre; CA>GFPstop>HA-UPRT transgenic cross
5-6 hours after an injection of 4-thiouracil (SC), the visual cortexes of four (littermate) Sepw1(NP39):cre, CA>GFPstop>HA-UPRT double transgenic mice per sample were dissected. RNA was then extracted and streptavidin purified, cDNA was generated using a SMARTER kit (v3), the library was prepped using the Nextera XT kit, and the sample was then Illumina sequenced.