RKO-DMSO-2
Human colorectal cancer cell line RKO which was trypsinized at 24h after 50μM prometryn pretreatment and total RNA was extracted using the trizol extraction method. The cDNA libraries were prepared from high quality RNA using an Illumina TruSeq RNA sample prep kit following the manufacturer’s instructions (Illumina, San Diego, CA, USA). Adaptors were ligated to the cDNA which was then enriched by PCR.