BCR amplicon sequencing Homo sapiens : adult male human PBMCS
Total RNA was extracted from PBMCs and then RT-PCR was utilized to generate B cell recptor (BCR) amplicon libraries using BIOMED2 primers with molecular amplification fingerprinting (MAF) error correction. Subsequent amplicon libraries were sequenced using next-generation sequencing. Libraries were sequenced using a VUMC core facility (VANTAGE).