Sediment 24 to 27 cm
Sedimented soil was collected from the culture pond in Rongcheng , Shandong province (36.9102010N, 122.2424850E) about 24-27 cm depth, and was subjected to DNA isolation by power soil DNA isolation kit. The sequencing of the isolated DNA was performed with the Illumina Miseq 16s rRNA sequencing in order to get a vertical variation of bacterial community influenced by sea cucumber (Apostichopus japonicas).