Whole Genome Sequencing of Vitis aestivalis cv. Norton
DNA was extracted in June of 2011 from young leaves of vine 516 (referred to as Norton-MSU1) which was propagated by green wood cutting from a heat-treated and virus tested mother vine (VFM123). the extraction protocol used was described Michiels et al.,Porebskie et al., and Lodhi et al. DNA in the amount of 200mg with a OD260/280 ratio higher than 1.6 was used in the 454 library preparation according to the manufacturer's instructions (Roche Diagnostics) at SeqWright in Houston, TX.