Mesophilic anaerobic digestion sludge: Sample N7CSEl
The DNA in fractions were analyzed using high-throughput sequencing on an Illumina platform (Illumina Miseq PE250). The V4-V5 region of the microbial 16S ribosomal RNA gene was amplified by PCR using the primers 515F (5’-GTG CCA GCM GCC GCG GTA A-3’) and 806R (5’-GGA CTA CHV GGG TWT CTA AT-3’), which were selected as the sequencing primer set to simultaneously obtain bacterial and archaeal information (Caporaso et al. 2011, Bates et al. 2011). The pretreatment and sequencing procedure was similar to the method used by (Amato et al. 2013).