picocyanobacterial diversity in the ECS in spring 2011
To elucidate picocyanobacterial ITS sequence diversity in the East China Sea, barcoded amplicon pyrosequencing with GS-FLX Titanium (454 Life Sciences, USA) was conducted. To amplify partial picocyanobacterial ITS sequences, barcoded primers were used. The barcoded primers consisted of adapter A (forward) or B (reverse)   Key (TCAG)   barcode (10-mers, forward only)   picocyanobacteria-specific primers. The ITS-af (5’-GGA TCA CCT CCT AAC AGG GAG-3’) and ITS-ar (5’-GGA CCT CAC CCT TAT CAG GG-3’) primers were used as specific primers. -------------------------------------------------- The locations of 23 samples obtained in this study. station Longitude (oE) Latitude (oN) B1 124 32 B2 124.5 32 B5 125.183 32.108 B7 126 32 D1 124 33 D3 124.5 33 D5 125 33 D8 126 33 H1 127.5 34.23 B9 126.75 32 D10 127 33 H3 127.5 33.75 H6 127.5 33