16S rDNA sequencing of bacteria: Microbial community in Synechococcus co-culture
The bacterial hypervariable V3-V4  region of 24S rRNA gene was amplified. Sequencing libraries were constructed using the NEB Next Ultra DNA Library Prep Kit for Illumina (NEB, USA) according to the manufacturer's recommendations. The library quality wasassessed with a Qubit 2.0 Fluorometer (Thermo Scientific) and Agilent Bioanalyzer 2100 system.