Clostridium thermocellum 27405 RNA Seq (Illumina HiSeq2000) from Populus and Switch grass fermentations
Total RNA was depleted of ribosomal RNA (rRNA) with the Ribo-Zero rRNA removal kit for Gram Positive bacteria (Epicentre,WI) following the manufactures instructions.  Libraries were prepared with the rRNA depleted material using the ScriptSeq mRNA-Seq Library Preparation kit (Epicentre,WI) following the manufacturer’s instructions.  Libraries were assessed with an Agilent bioanalyzer High Sensitivity Assay (Agilent, CA).