MDA-MB-231_T83_1
Small  RNA sequencing  (SmRNA )  library  was prepared  with  NEXTflex  Small  RNA Sample Preparation  protocol ( Bioo   Scient f i c  Corporation ,   Austi n,  Texas, U.S.A.)   at Genotypic   Technology   Pvt.   Ltd.,  Bangalore, India.  5 00 n g of  Total  RNA  was  used as  starting material .  3 adapte rs were ligated to the specific 3OH group  of  micro RNAs followed by  ligation of 5 adapte r .  Adapter  ligated fragments  were reverse transcribed  with  M - MuLV Reverse t ranscriptase by priming with  r everse transcript ion primers.  cDNA thus formed  was enriched and barcoded by PCR amplification (15 cycles).  Library  was size  sel ected  using  NEXTflex  beads  Cleanup .  Illumina - compatible  sequencing  library  was  quantified by Qubit fluorometer (Thermo Fisher Scientific, MA, USA) and its fragment size distributi on  was analyzed on  D1000 Tape Station.