RNA-Seq of T. cruzi epimastigotes: G2/M-phase enriched
Total RNA was obtained using TRIzol  Reagent (Invitrogen ), treated with DNAse (DNA-free, Ambion).  Equimolar amounts of RNA derived from the three independent synchronizations (biological replicates) were pooled. Poly-A+ mRNAs were isolated by oligo-dT selection and single-end 50 bp sequencing libraries for RNA-seq analysis were built on standard Illumina RNA-seq protocols. Sequencing experiments were performed on an Illumina  HiSeq  2000 platform (service provided by BGI Americas).