Cas9 RNP with ssODN HPRT-087 6nt ins 30nt HA_PS modified 2
Targeted loci were amplified with a single primer pair; subsequently, universal amplification was used to install unique index sequences. Illumina sequencing was carried out on a MiSeq using V2 chemistry and 2x150 paired-end reads. Primer sequences and protocols are described in the manuscript.