Sequencing of NA12878 on Ion Proton
In total, 1 g of DNA (100ng/l) was fragmented using ion shear plus reagents. The fragmented library was size selected using AMpure beads to get a 130bp peak on bioanalyser. The adaptors ligated into fragmented genomic DNA, and the library was amplified. Adaptor-ligated library was hybridized with biotinylated RNA library baits for 16 hours, enriched with streptavidin-coated magnetic beads. Streptavidin bound DNA fragments were selected using a magnet and amplified for 11 cycles. The concentration of final libraries measured using quantitative real-time PCR followed by template enrichment using ion chef.