Mock Even community 16S 454 sequencing to test HMP protocols.
Pooled DNA from 20 microbes (Organism (strain#)-Target 16S copies in PCR: Acinetobacter baumanii (ATCC       17978)-100000, Actinomyces odontolyticus (ATCC 17982)-100000, Bacillus cereu s (ATCC 10987)-100000, Bacteroides vulgatus (ATCC       8482)-100000, Candida albicans (SC5314)-1120, Clostridium beijerinckii (ATCC 51743)-100000, Deinococcus radiodurans (DSM       20539)-100000, Enterococcus faecal is (ATCC 47077)-100000, Escherichia coli (ATCC 70096)-100000, Helicobacter pylori (ATCC       700392)-100000, Lactobacillus gasseri (DSM 20243)-100000, Listeria monocytogenes (ATCC BAA-679)-100000, Methanobrevib acter smithii       (ATCC 35061)-100000, Neisseria meningitidis (ATCC BAA-335)-100000, Propionibacterium acnes (DSM 16379)-100000, Pseudomonas aeruginosa       (ATCC 47085)-100000, Rhodobacter sphaeroides (ATCC 17023) -100000, Staphylococcus aureus (ATCC BAA-1718)-100000, Staphylococcus       epidermidis (ATCC 12228)-100000, Streptococcus agalactiae (ATCC BAA-611)-100000, Streptococcus mutans (ATCC 700610)-100000,       Streptococc us pneumoniae (ATCC BAA-334)-100000) at approximately even concentrations, over 5 orders of magnitude, based on 16S DNA       concentrations.This mock microbiome community was designed to test HMP sequencing and analysis protocols.