RNA-seq of mouse Lewis lung carcinoma P29mtB82M cybrid cells
Total RNA was extracted from P29mtB82M cells using RNAeasy Plus Mini Kit, with quality and quantity assessed onn an Agilent 2100 BioAnalyzer and RNA 6000 Nano Kit (criteria: 5 g of total RNA with RNA integrity  7); after depleting ribosomal RNA with RiboMinus Eukaryote System v2, the sample was barcoded with Ion Total RNA-Seq kit v2.0, quantified (High Sensitivity DNA Kit) and subject to emulsion PCR and sequenced on an Ion Torrent Proton sequencer.