Spleen genome DNA methylation sequencing of grass carp infected with Aeromonas hydrophila
Briefly, genomic DNA was fragemented (Covaris S220, USA), purified (SPRI Beads, USA), end repaired, A tailed, and ligated with adapter. Then the products were prepared for conversion using the EZ DNA Methylation-GoldTM Kit (ZYMO RESEARCH, USA), and cleaned up. Finally, the library was amplified using KAPA HiFi Hotstart Utracil+ ReadyMix (2 ) (KAPA Biosystems, USA), and cleaned up too