Botryococcus braunii UMA2 mRNA sequence 454 sequencing
The microalgae biomass was collected from massive cultures of B. braunii race A (UMA2) on flat-panel type photobioreactors of 1m3 on the days 20-25 of culture, corresponding to log phase and the beginning of the stationary stage of the microalgae, and the stage with the largest accumulation of lipid, close to a 30% [21]. Total RNA was extracted using the trizol and phenol-chloroform procedure described in [22] with some modifications. The mRNA was extracted with the Roche Applied kit (Germany), using 45 *g of total RNA. The mRNA quantification was determined by fluorometry, using the Bioanalyzer 2100 (Agilent, USA). For the construction of the library, 200 ng of mRNA were used.