Illumina WGS of 2010EL-1786
Cholera genome sequencing was performed using Roche Illumina Genome Analyzer chemistry in the CDC Biotechnology Core Facility. For each genomic DNA a random shotgun library was produced using standard protocols for nebulization, end-polishing and adaptor ligation and size selection. Illumina libraries were flowcell amplified using a cBot single read cluster generation kit. These were then sequenced using v4 cycle sequencing chemistry.