MiSeq Sequencing Data for NIST RM 8378 Clostridium sporogenes - Vial 4 Rep 2
Independent duplicate libraries were constructed from 8 replicate Clostridium sporogenes microbial genomic DNA reference material (RM8378) vials for a total of 16 libraries, the libraries were pooled and sequenced in a single run. Libraries were prepared using the Nextera DNA Sample Prep Kit (FC-121-1030). The 8 vials were barcoded using the Nextera Index Kit (FC-121-1011, lot: 9988423), vial numbers correlate with Ion Torrent data vial numbers. Libraries were sequenced using the the MiSeq 600 cycle Reagent kit v3 (MS-102-3003, box 1 lot:10064179; box 2 lot:10065188). Fastq generated using the MiSeq Reporter version 2.5.1.3 with the GenerateFASTQWorker workflow Version 0.0.0. Vial 4 Rep 2 barcoded: i5 index- N503, i7 index - N702.