Transcriptome-based phylogeny of ticks of the genus Ixodes
Ticks (Ixodes holocyclus) were sampled in 2016, in Queensland, Australia (by Dr Steve Barker). Ticks were found on  the vegetation by flagging  and sent in RNAlater to the lab (INRA/Oniris/BIOEPAR Nantes, France). RNA extraction was performed in 2016 (whole bodies of unengorged adult females). RNA was  extracted using a NucleoSpin RNA XS column (Macherey-Nagel, Dren, Germany) including a DNase and was stored in RNAsin (Promega, Madison, USA). The library was prepared using the Truseq Stranded kit (v4), and was sequenced on one lane of Illumina HiSeq 2500 along with other tick samples (different species of the genus Ixodes).