Exome Seq of homo sapiens liver
Demultiplexing was performed with Illumina CASAVA (1.8.2) and adapters were trimmed with Skewer (0.1.116). Trimmed raw reads were aligned to the human genome (hg19) with the Burrows-Wheeler Aligner (BWA-mem version 0.7.2). Reads that aligned at more than one locus were discarded. Duplicate reads were removed with SAMtools (0.1.18). To enhance sensitivity, two different software tools were used for variant calling: SAMtools and varscan (2.3.5).