Adult mice treated with mianserin (4)
Adult mice were treated with mianserin(acute, 20mg/kg). Samples containing the rostral part of the caudate putamen and the nucleus accumbens were collected. High-quality RNA samples (RIN>8) from 3 animals were pooled in equal concentrations and 5 µg of total RNA was used for rRNA depletion with the RiboMinusTM Eukaryote Kit. Library size selection was performed by excision of bands within a range of 150-250 nucleotides from a TBE-urea gel. The cDNA isolated from these bands was amplified using SOLiD 5500XL-compatible barcoding primers and the AmpliTaq® polymerase. The libraries were templated onto beads using full-scale emulsion PCR (ePCR). Templated beads were used for high-throughput sequencing on a SOLiD 5500XL system (50+35bp PE).