SKCT13_b2_21
Samples were collected by moistening cotton tip swabs (VWR, Milan, Italy) in SCF-1 sample buffer (50 mM Tris-HCl, pH 7.5; 1 mM EDTA, pH 8.0; 0.5% Tween-20) and swabbing the external elbow skin area for 30 seconds. To recover the sample the head of the swab was pushed against the side of sterile collection tube. Samples were pre-treated for 30 minutes at 37oC in a lysis solution (20 mM Tris-HCL, pH 8.0; 2 mM EDTA; 1 % Triton X-100) supplemented with Lysozyme (final concentration 20 mg/ml) (Sigma-Aldrich, Milan, Italy) before DNA was isolated with the Mo-Bio PowerSoil DNA isolation kit (Mo Bio laboratories, Carlsband, CA,USA) as previously described. Libraries were prepared using Illumina Nextera-XT DNA kit (Illumina inc, San Diego, CA, USA) as per manufacturer’s instructions. Libraries were pooled and sequenced (100 bp paired end) on the Illumina HiSeq-2000 platform. The study was approved by the Ethical Committee of the University of Trento and informed written consent was obtained from all volunteers.