RNA-Seq of Quercus cornelius-mulleri: SY-1 leaf from wild
Samples were frozen on dry ice in the field and stored at -80 deg C until total RNA extraction. Preliminary RNA precipitation (http://openwetware.org/wiki/Conifer_RNA_prep) was performed prior to total RNA extraction with the Qiagen RNeasy Plant Mini Kit protocol with DNase treatment. RNA-Seq libraries with insert length 100 - 380 bp (mode = 170 bp) were prepared from 4 g of RNA using an Illumina TruSeq RNA Sample Prep Kit. Each library was uniquely tagged using 12 TruSeq indexed adapters (numbers 1 - 12) to enable 12-plexing of samples in each Illumina HiSeq 2000 lane.