Gossypium hirsutum fiber transcriptome: Gossypium hirsubum var. yucatanense TX2090 10DPA
Total RNA was extracted using either Hot borate method or a CTAB extraction protocol, and then purified with the Qiagen RNeasy Plant Mini Kit. The quality and concentration of extracted RNAs were determined using the Agilent 2100 Bioanalyzer. mRNA purification was performed using the MicroPoly(A) PuristTM kit, and then RNA-Seq library was constructed using NEBNext mRNA Library Prep Reagent Set for Illumina.