mRNAseq of specific Brassica napus accession: Sample BnASSYST-113
"The libraries were normalised and equimolar pooled to 10 nM using elution         buffer (Qiagen) . The library pool was then diluted to 2 nM with NaOH and 5?L transferred         into 995?L HT1 (Illumina) to give a final concentration of 10pM. Each pool was spiked with         1% PhiX Control v3 and the flow cell was clustered using TruSeq SE Cluster Kit v3-cBot-HS.         After the clustering procedure, the flow cell was loaded onto the Illumina HiSeq 2500         instrument following manufacturer's instructions. The sequencing chemistry used was TruSeq         SBS Kit v3-HS using HiSeq Control Software 2.2.58 and RTA 1.18.64. Reads in bcl format were         demultiplexed based on the 6 bp Illumina index by CASSAVA allowing for a one base-pair         mismatch per library, and converted to FASTQ format by bcl2fastq."