Investigation of Curcumin-induced Transcriptome Profile of Non-small Cell Lung Cancer A549 Cells using RNA-seq
Experimental Design: A549 cancer cell lines with / without Curcumin treatment. 8μg/ml, 48hr. RNA extraction and Oligo-dT purified mRNA, Fragment the mRNA and reverse transcript with ramdom primer. Library generated by PCR. Sequence with Hiseq2000 platform. Sequence strategy: PE100, ~6G Data.