genome sequencing Pseudomonas sp. 4B
Total DNA from the bacteria strains were extracted by lysis with cetyltrimethylammonium bromide (CTAB) and isolation by the addition of phenol: chloroform. DNAs quality and quantity was analyzed in NanoDrop instrument and Qubit,  respectively. DNA libraries were prepared with Nextera DNA Library Kit and the high-throughput sequencing was performed on Illumina MiSeq system  to generate 150-bp paired end reads (v2 chemistry) according to the manufacturers protocol.