Hordeum brevisubulatum(Trin.)Link of RNA-Seq
After extracting total RNA from the sample, the eukaryotic mRNA is enriched with magnetic beads with Oligo (dT), and the appropriate mRNA is added to the obtained mRNA to fragment it under high temperature conditions, and the broken mRNA is used as a template for synthesis. One-strand cDNA, then configure the two-strand synthesis reaction system to synthesize the two-strand cDNA, and use the kit to purify and recover, the sticky end repair, the 3' end of the cDNA plus the base "A" and connect the linker, then select the fragment size, and finally Perform PCR amplification;