16S rRNA sequencing of human fecal donor samples
16S rRNA gene sequencing was performed at the University of Nebraska Medical Center Genomics Core Facility. DNA normalization and library prep were performed followed by V3-V4 16S rRNA amplicon gene sequencing using a MiSeqV2 (Illumina) The following primer sequences were used: (Primer sequences: Forward Primer = 5'TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG 16S Amplicon PCR Reverse Primer = 5' GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCCIllumina overhangs: Forward overhang: 5 TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG[locusspecific sequence] Reverse overhang: 5 GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG[locusspecific sequence]).