targeted sequencing of a uveal melanoma lesion: Sample P136.fmi
DNA was extracted from routine FFPE biopsy or surgical specimens, 50200 ng of which underwent whole-genome shotgun library construction and hybridization-based capture of 4,557 exons from 287 cancer-related genes and 47 introns from 19 genes frequently rearranged in solid tumors. Hybrid-captureselected libraries were sequenced to high uniform depth (targeting >500× coverage by non-PCR duplicate read pairs, with >99% of exons at coverage >100×) (Frampton et al., 2013)