RNA-Seq of Scyliorhinus canicula vertebrae: embryo stage 32
The library was prepared from 1.2 g of total RNA, from which the Poly(A)+ mRNA fraction was isolated using poly-T oligo-attached magnetic beads, then chemically fragmented and finally reverse transcribed to generate cDNAs that are ligated to adapters. These products are purified and amplified with PCR to generate the indexed final double stranded cDNA library.