Mock Staggered community 16S 454 seuqncing to test HMP protocols.
Pooled DNA from 20 microbes (Organism (strain#)-Target 16S copies in PCR: Acinetobacter baumanii (ATCC 17978)-10000, Actinomyces odontolyticus (ATCC 17982)-1000, Bacillus cereus (ATCC 10987)-100000, Bacteroides vulgatus (ATCC 8482)-1000, Candida albicans (SC5314)-1000, Clostridium beijerinckii (ATCC 51743)-100000, Deinococcus radiodurans (DSM 20539)-1000, Enterococcus faecalis (ATCC 47077)-1000, Escherichia coli (ATCC 70096)-1000000, Helicobacter pylori (ATCC 700392)-10000, Lactobacillus gasseri (DSM 20243)-10000, Listeria monocytogenes (ATCC BAA-679)-10000, Methanobrevibacter smithii (ATCC 35061)-1000000, Neisseria meningitidis (ATCC BAA-335)-10000, Propionibacterium acnes (DSM 16379)-10000, Pseudomonas aeruginosa (ATCC 47085)-100000, Rhodobacter sphaeroides (ATCC 17023)-1000000, Staphylococcus aureus (ATCC BAA-1718)-100000, Staphylococcus epidermidis (ATCC 12228)-1000000, Streptococcus agalactiae (ATCC BAA-611)-100000, Streptococcus mutans (ATCC 700610)-1000000, Streptococcus pneumoniae (ATCC BAA-334)-1000) at staggered concentrations, over 5 orders of magnitude, based on 16S DNA concentrations.This mock microbiome community was designed to test HMP sequencing and analysis protocols.