A. chinensis fed on pupae of Chinese oak silk moth
Total RNA was extracted using the RNAiso Plus (TaKaRa, Dalian, China) according to the manufacturer’s protocol.RNA integrity was confirmed using the 2100 Bioanalyzer (Agilent Technologies) with a minimum RNA integrated number value of 8. According to the Illumina manufacturer’s instructions, poly(A)+ RNA was purified from 10 µg of pooled total RNA using oligo(dT) magnetic beads and fragmented into short sequences in the presence of divalent cations at 94? for 5 min. The cleaved poly(A)+ RNA was transcribed, and then second-strand cDNA synthesis was performed. After the end-repair and ligation of adaptors, the products were amplified by PCR and purified using the QIA quick PCR purification kit to create a cDNA library. The cDNA library was sequenced at The Beijing Genome Institute (Shenzhen, China) on the Illumina sequencing platform (HisSeqTM 2000). The sequencing machine generated the raw images which were controlled by HCS system. The raw images were transformed into BCL file by RTA, then the BclConvert software was used to generate raw reads from BCL.