16S barcoding of archaeal communities living in mountain lake sediments
The universal primer A519F (S-D-Arch-0519-a-S-15, 5 -CAG CMG CCG CGG TAA-3 ) and archaeal specific primer 1017R (S-D-Arch-1041-a-A-18, 5 -GGC CAT GCA CCW CCT CTC-3 ) were used to amplify a ~540 bp fragment corresponding to the V4-V5-V6 region of the 16S rRNA gene