miRNA-Seq Ago2ko_PBS_3
RNA was extracted by miRNeasy micro Kit (Qiagen) with total RNA extraction protocol. NEBNext small RNA library preparation kit (New England BioLabs, Ipswich, MA) was used for library preparation. Libraries at the final concentration of 10 pM were clustered onto a single read (SR) flow cell using Illumina TruSeq SR Cluster kit v3, and sequenced for 50 cycles using TruSeq SBS kit on Illumina HiSeq system.