mESCs cultured in R2i
Small RNA sequencing using Illumina platform was performed as follows: Total RNA was extracted from the mESC samples and used to generate small RNA libraries from which cDNA libraries were prepared at LC Sciences. The purified cDNA libraries were then used for cluster generation on Illumina’s Cluster Station and then sequenced on Illumina GAIIx (Illumina HiSeq 1000/Illumina GAIIx) according to the vendor’s instruction for running the instrument. Raw sequencing reads (up to 45 nt) were obtained using Illumina’s Sequencing Control Studio software version 2.8 following real-time sequencing image analysis and base-calling by Illumina's Real-Time Analysis version 1.8.70 (LC Sciences).