RNA-Seq of Fornicata sp. IC-2016a
Cells were cultured at room temperature for seven days before RNA extraction. Cells from 50 ml of the culture were pelleted by double centrifugation (1,200 g for 8 min and 2,000 g for 12 min). Total RNA was extracted using TriReagent Solution (Ambion, USA) according the manufacturer's instructions and mRNA was isolated by double polyA selection with Dynabeads Oligo(dT)25 (Invitrogen, USA). Illumina RNAseq libraries were prepared from mRNA using the NEXTflex RNASeq Kit (developed by BIOO Scientific) and 150 bp paired end reads were sequenced on the MiSeq platform and quality-filtered by the Genomics Core Facility, EMBL, Heidelberg.