RNA-seq of Scolopendra subspinipes venom gland
Total RNA was extracted by standard TRIzol protocol (ThermoFisher, USA) and enriched for polyA RNA using a DynaBeads Direct mRNA kit (ThermoFisher, USA). The two polyA enriched RNA samples were submitted to the University of Queensland Institute for Molecular Bioscience Sequencing Facility for library preparation and sequencing. Paired end libraries with 180 bp insert size was constructed using the Illumina TruSeq -3 Stranded mRNA kit and sequenced on an Illumina NextSeq using a 300 cycle (2x150bp) Mid Output Run.