RNA-seq of SM482gs and SM482
The mutant line SM482gs with increased grain size was selected from the common wheat cultivar Shumai 482.Grains of SM482gs and SM482 were harvested at 9, 15, 20, 25 days post anthesis (DPA).For each developmental stage, RNA from three individual plants was pooled, and two biological replicates for RNA-seq analysis were prepared for each stage. In total, 16 RNA samples were subjected to reverse-transcribe and sequenced on an Illumina Hiseq.RNA-seq reads were mapped to the reference genome for common wheat (cultivar Chinese Spring, downloaded from the International Wheat Genome Sequencing Consortium (IWGSC) database.