Oddjob ChIP-seq replicate 2
S2 cells transfected with either mCherry-Nnk or Odj-mCherry for 72 hours were fixed with 1% paraformaldehyde for 10 minutes and sheared with Bioruptor sonicator (Diagenode) to obtain chromatin. Chromatin fragments were confirmed to contain DNA in the 200-500 bp size range using a Bioanalyzer (Agilent). Each immunoprecipitation was performed on chromatin from 2x107 S2 cells by overnight incubation with Protein-G Dynabeads and 5 ug of anti-mCherry antibody (Novus). Library construction from immunoprecipitated DNA was conducted using TruSeq sample preparation kits (Illumina). 150bp paired-end sequences were generated by the Vincent J. Coates Genomics Sequencing Laboratory at UC Berkeley.