RNA-Seq of Solanum lycopersicum leaves: leaves treated three times with 12.5 g/l COS-OGA
Plant leaves  were harvested 24 hours after the third spaying. RNA extraction was performed on 100 mg ground nitrogen-frozen leaves using NucleoSpin RNA Plant (Macherey-Nagel. RNA concentration, purity and integrity were assessed with Bioanalyzer 2100 . RNA samples were sent to VIB Nucleomics Core (Leuven, Belgium) for sequencing. Library preparation was made with TruSeq Stranded mRNA kit (Illumina, USA) on 1 g total RNA. Libraries were then sequenced on NextSeq 500 Platform (Illumina, USA) following standard protocol (75 bp single-end reads, High-Output kit, 1 X 75 cycles).