2C-ChIP of NT2D1: 72h RA rep B
2C-ChIP forward and reverse primers are used for ligation-mediated amplification of target sites on ChIP DNA library. After ligation, 2C-ChIP library is amplified using universal primers (designed based on sequencing platform, with a barcode on the forward primer). Amplicons are then size selected with magnetic beads (SERA MAG beads) and quantified with the Ion Library TaqManTM Quantitation Kit.