RNA Seq of Solanum habrochaites PI251305-902: style
Solanum habrochaites plants were grown to the flowering stage.  Approximately 30 styles were harvested directly into RNA later and RNA was extracted using the Qiagen RNeasy plant mini-kit.  cDNA libraries were sequenced the results were used to identify genes highly expressed in stylar tissue that may be important in self and unilateral incompatibility.Three individuals of PI251305 were used as biological replicates. The RNAseq libraries were generated using the TruSeq Stranded mRNA kit protocol. In brief, first the integrity of the RNA was checked with Agilent Tapestation, samples with RIN >7 were used for library preparation. The mRNA was purified from 1ug of total RNA for each sample, after cDNA synthesis and adapter ligation, the library was amplified with 12 rounds of PCR cycles. The enriched and cleaned libraries were pooled and loaded on the sequencer.