HLA Typing Dev on 454, HapMap 270
270 HapMap human genomic DNA samples of known HLA type were amplified by PCR with six different exon-specific primer pairs.  The six amplicons for each individual were pooled, and underwent barcoded 454 library construction.  A total of 96 unique barcodes were utilized, thus, sets of 96 individuals could be pooled prior to sequencing.