Ciona follicle Stage IV_replicate2
Total RNA from isolated follicles were extracted, purified, and treated with TURBO DNase (Thermo Fisher Scientific, USA). The quality-checked total RNA by the BioAnalyzer (Agilent Technologies, USA) was subjected to the library construction using TruSeq Stranded mRNA Sample preparation kit (Illumina, USA), according to the manufacturers instructions. 101-cycle of single-end sequencing was performed using HiSeq1500 (Illumina) in the rapid mode. Total reads were extracted with CASAVA v1.8.2 (Illumina).