High-throughput RNA sequencing of a FFPE autopsy lung tissue samples from the 2009 influenza pandemic
The construction of total RNA libraries from FFPE samples was performed according to Illumina mRNA-seq sample preparation kit (Illumina, San Diego, CA) specifications but skipping the poly-A selection and RNA fragmentation steps. Then, 2 rounds of Duplex-Specific Nuclease (DSN) (Evrogen, Moscow, Russia) treatments on the library were performed according to the Illumina DSN normalization procedure.