RNA-seq of homo sapiens CLL PBMCs: Sample SA24823
RNA was extracted from peripheral blood mononucleocytes from CLL cases per the manufacturer’s specifications using Qiagen RNeasy mini-spin columns. RNA was polyA selected and processed using SMART cDNA synthesis (Clontech) to prepare sequencing libraries. Sequencing adapters and poly-A/T tails were trimmed for CLL samples only using cutadapt version 1.1. Read pairs where or one of both reads were of length less than 20 were removed.