RNA-seq of mus musculus visual cortex, streptavidin purified from a WT mouse (background, contaminating unlabeled or mislabeled RNA)
5-6 hours after an injection of 4-thiouracil (SC), the visual cortexes of four WT mice per sample were dissected. RNA was then extracted and streptavidin purified, cDNA was generated using a SMARTER kit (v3), the library was prepped using the Nextera XT kit, and the sample was then Illumina sequenced.