RNA-Seq of Homo sapiens: second trimester chorionic villi
RNA was isolated from cultures using the GenElute mammalian total RNA miniprep kit (Sigma) and the associated Dnase digestion set (Sigma). Both a NanoDrop spectrophotometer and an Agilent bioanalyzer were used to determine sample quality. Sequencing libraries were prepared from 0.2 to 0.9 g of total RNA with the TruSeq stranded mRNA library preparation kit (Illumina).