Single cell RNA-seq 10% RH infected
Single cell suspensions were loaded onto the  Chromium Controller (10x Genomics) for droplet formation. Single-cell RNA-seq  libraries were prepared using the Chromium Single Cell 5 Reagent Kit (10x  Genomics) and Single Cell V(D)J Kit according to manufacturers protocol.  Samples were sequenced on the HiSeq4000 with 28 bp Read 1, 8 bp i7 index and  98 bp Read 2 for the gene expression library, and on the NovaSeq with 150  paired reads for BCR library.