"TEX and TAP untreated RNA from Methanocaldococcus jannaschii: library 1, replicate 1."
"RNA samples isolated from M. jannaschii cell pellet and treated with Terminator exonuclease (TEX) then split in two with one half being treated with tobacco acid pyrophopatase (TAP, Tplus, untreated = Tminus). cDNA was prepared and further fragmented by ultrasound and libraries produced which included size fractionation to 220-600bp (including sequencing adaptors). Sequences were alingned to M. jannaschii genome with default parameters except -n 0 -m 1 -5 2 (to trim off extra nuclotides added to improve efficiency of adapter ligation). "