Multi-genomic profiling of EndoC_BH1 beta cells and human pancreatic islets
Total RNA was extracted and purified using Trizol (Life Technologies). miRNA libraries were prepared at the NIH Intramural Sequencing Core (NISC) from 1 g total RNA using Illuminas TruSeq Small RNA Library Kit according to the manufacturers guidelines, except a 10% acrylamide gel was used for better separation of library from adapters. Libraries were pooled in groups of about 8 for gel purification. Single-end 51 base sequencing was performed on Illumina HiSeq 2500 sequencers in Rapid Mode using version 2 chemistry.  Data was processed using RTA version 1.18.64 and CASAVA 1.8.2. All resulting data was processed with miRquant 2.0 (Kanke et al., 2016).