RNA-Seq of R.crenulata: tissue culture seelding
The sequencing library was constructed using polymerase chain reaction. The multiplexed cDNA libraries were checked using PicoGreen (Quantifluor-ST fluorometerE6090, Promega, CA, USA) and fluorospectrophotometry (Quant-iT PicoGreen dsDNA Assay Kit; Invitrogen, P7589) and quantified with Agilent 2100. The synthesized cDNA libraries were normalized to a 10 nM. The sequencing libraries were gradually diluted and quantified to 4-5 pM and sequenced using the Illumina NextSeq 500 platform