miRNAome of roots of sensitive maize under severe drought
Small RNA sequencing libraries were constructed by using Next Ultra small RNA Sample Library Prep Kit for Illumina (NEB, USA). In brief, small RNA molecules were ligated with 3' and 5' adapter by T4 RNA Ligase. Subsequently, RNA samples were reverse transcribed and amplified by PCR. Then the cDNA was separated by 6% polyacrylamide (PAGE) gel electrophoresis and the short molecules were recovered and purified.