ITS region amplicons of Sceloporus grammicus fece's. Adult male.
The fungal internal transcribed spacer (ITS) ITS1-5.8S-ITS2 region was amplified with the primers ITS1F  and ITS4R. Primers used contained the adapter for sequencing platform and 8 nt barcodes. Amplification reactions were done in quadruplicate, pooled and purified using QIAquick PCR purification kit according to manufacturers instructions. The quantification of the PCR products was done using NanoDrop 3300 fluorospectrometer with PicoGreen dsDNA assay and combined in equimolar quantities for sequencing by Macrogen Inc.