Whole genome shotgun sequencing GM20847
200ng of HMW gDNA from individual GM20847 was subjected to simultaneous fragmentation and adaptor  ligation followed by PCR amplification with the Nextera DNA sample prep kit as directed by the  manufacturer. The resulting product was purified with 1.8 volumes AMPure XP beads, electrophoresed,  size selected, cleaned up, and reamplified by limited-cycle PCR as for fosmid pool-derived libraries. The  resulting library was sequenced using an Illumina HiSeq instrument (50-bp paired end reads).