RNA-seq of C. elegans: empty vector germline
RNA-seq on micro-dissected full germlines from N2 hermaphrodite C. elegans treated with empty vector control, and collected at initation of reproduction (day 2.5 of life). Poly-A selected RNA-seq libraries were generated on 20 full germlines using the Clontech SMART-Seq v4 Ultra Low Input RNA Kit for cDNA synthesis (634889) and the Illumina Nextera XT DNA Library Prep Kit for library construction (FC-131-1024).