RNA seq of Arabidopsis thaliana: 5-day-old siliques
RNA-Seq was conducted on green siliques of Arabidopsis plants grown in soil in a growth chamber at 8 days after flowering (DAF) at 22 degree under long day conditions (16 hours of light/8 hours of dark). Total RNA from 8-day-old siliques of one plant was extracted for each biological replicate and purified. Two biological replicates were used for each genotype and the WT controls. Transcriptome sequencing (an Illumina HiSeq 2000 system with V3 reagent, 91 pair-end sequencing) were conducted at BGI China.