GeCKOv2 Screening of stomach organoid
The primer sets HT-gLibrary-Miseq_150bp-PE-F: TCGTC GGCAGCGTCAGATGTGTATAAGAGACAGcttgaaagtatttcgatttcttgg and HT-gLibrary-Miseq_150bp-PE-R: GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGactcggtgccactttttcaa were used to amplify the gRNA locus. The PCR condition was 95 C (20 sec), 55 C (20 sec), and 72 C (30 sec) for 26 cycles. Q5 Taq polymerase was used. Secondary PCR was performed using Nextra XT index primers. The secondary PCR condition was 95 C (20 sec), 55 C (20 sec), and 72 C (30 sec) for 12 cycles. PCR products were purified using a PCR purification kit.