Partial Genome sequence of Salmo trutta fario
Illumina platforms used to prepare multiplexed libraries for paired-end sequencing (2300bp PE). For high-throughput sequencing, libraries were generated using NEXTflex DNA Sequencing Kit 2.0 (Bio Scientific Austin, USA) USA) following manufacturers recommendations. 3 g purified DNA was used to prepare sequencing libraries and approximately 600-800 base pairs fragments were generated. After the quality analysis PCR was performed using the primers provided in the NEXTFlex DNA Sequencing kit. The PCR products were purified and quantified. The sequencing was carried out on Illumina Miseq platform at Genotypic Technology Pvt Ltd, Bangalore, IND.