RNAseq Aethionema arabicum: dehiscent seed rep 1
Aethionema arabicum (L.) A.DC. accession 0000309 (obtained from Kew's Millennium Seed Bank, UK) plants were grown on soil under long-day conditions (16 h light/20°C and 8 h dark/18°C). Freshly matured seeds from dehiscent and indehiscent fruits derived from several plants were harvested. Two replicates of 20 mg fresh dry M+ and M- seeds, resulting in four samples in total, were pulverized in liquid N2 using mortar and pestle. RNA extraction was performed according to (Graeber et al., 2011).