Silty_Clay_Loam_Fertilizer_Control_R3
Greenhouse radish taproots were grown to maturity, and surface bacterial cells were dislodged into solution (0.1% each peptone & Tween 80). Cells were collected on 0.22-?m mixed cellulose membranes for DNA extraction (MPBio FastDNA Spin Kit for Soil, Zymo PCR inhibitor clean-up kit).  EMB protocol using 515Fb-926R was used for 16S rRNA gene amplicon sequencing.