Barley leaf RNA fraction, Golden Promise, transformed with Ta-DREB3
Total RNA was isolated using TRIzol and size fractionated by electrophoresis on a polyacrylamide gel. RNA fraction of 18-30 nt was excised from gel and adapters ligated to both ends.  After reverse transcription, resulting cDNA was sequenced in a separate lane of the Illumina Genome Analyzer.