ILLUMINA paired Custom hybrid capture (OncoPanel) sequencing of paired normal blood sample from meningioma patient
DNA was sonicated to achieve an average fragment size of 150bp, followed by double size-selection (Agencourt AMPure XP beads) and ligation to specific barcoded adaptors (Illumina TruSeq) for multiplexed analysis. Each multiplexed pool was hybridized with biotinylated baits (Agilent SureSelect, Agilent) designed to capture exonic sequences for 645 genes (OncoPanel). Streptavidin-coated beads were used to pull down the complexes, which were loaded on a HiSeq2000 for paired-end sequencing.