mRNA-seq of mus musculus: B16 WT cells
A total amount of 1 mg RNA per sample was used for the RNA sample preparations. Sequencing libraries were generated using NEBNext Ultra RNA library Prep Kit for Illumina (NEB) according to manufacturers recommendations and index codes were added to attribute sequences to each sample. The clustering of the index-coded samples was done on a cBot Cluster Generation System using HiSeq 4000 PE Cluster Kit (Illumia) following the manufacturers protocols. After cluster generation, the libraries were sequenced using Illumina Hiseq 4000 and 150 bp paired-end reads were generated.