NA12762 library “C2”, production lot 2
A paired-end linkered library was generated using the HapMap sample NA12762 following the Illumina Paired-End Genomic DNA Sample Prep Kit Protocol in conjunction with the Roche NimbleGen SeqCap EZ Exome Library SR Guide version 2.0. Briefly, 3ug of gDNA was fragmented using a Covaris E210 Water Bath Sonicator. Following fragmentation, the DNA was end-repaired, adenylated, and ligated to Illumina Paired-End Adaptors according to the Illumina Paired-End Genomic DNA Sample Prep Kit Protocol. The sample was then purified using an Agencourt AMPure XP Kit size selection per the Roche NimbleGen SeqCap EZ v2.0 protocol.  The purified paired-end library was amplified via LM-PCR (linker mediated-PCR) and was then hybridized to Roche NimbleGen SeqCap EZ Exome v2.0 solution-based oligo pool. Following hybridization, the captured DNA sample was amplified using LM-PCR as detailed in the Roche NimbleGen SeqCap EZ Exome Library SR Guide version 2.0 and was determined, via Agilent Bioanalyzer 2100 DNA 1000 Lab Chip, to have an average fragment size of 229bp (±7bp).