Whole genome sequencing of Pseudomonas phage vB_Pae_AM.P2
AM.P2 phage DNA was isolated using phenol-chloroform method. For Nanopore sequencing, library was prepared using 1D Ligation Sequencing Kit (SQK-LSK109) from Oxford Nanopore Technologies (ONT). Sequencing run was performed for an hour using the MinION device. Guppy, available in the MinKNOW software was used in high accuracy mode for basecalling. The final sequence output was obtained in fastq format.