Genome Sequencing of Streptochaeta angustifolia
For sequencing of S. angustifolia, leaf tissue was harvested and DNA isolated using Qiagen DNeasy plant kits. Three Illumina libraries (paired end and 9- and 11-kb mate pair) were generated from these isolations at the Iowa State University (ISU) DNA Facility. One lane of 150bp paired end HiSeq sequencing (insert size of 180bp) and one lane of 150bp mate-pair HiSeq sequencing (9- and 11-kb libraries pooled) were generated, also at the ISU DNA Facility. These two lanes of HiSeq produced 48x depth sequence of the S. angustifolia genome