16S seq of culture MSM day 28_50FLX
Ten ng of extracted DNA was used as template and the PCR reaction (25 uL) contained dNTPs (400nM of each), MgSO4 (1.5 mM), Platinum Taq DNA polymerase HF (2mU), 1X Platinum High Fidelity buffer (Thermo Fisher Scientific, USA), and barcoded library adaptors (400 nM) containing V1-3 specific primers: 27F AGAGTTTGATCCTGGCTCAG and 534R ATTACCGCGGCTGCTGG.