Genome sequencing of Leishmania macropodum
Total genomic DNA was isolated from 10 ml of trypanosomatid cultures with the DNeasy Blood & Tissue Kit according to the manufacturers instructions. SSU rRNA gene was amplified using primers S762 and S763, following the previously described protocol (Yurchenko). These PCR fragments were sequenced directly at Macrogen Europe, Amsterdam, Netherlands. The identity of species under study was confirmed by BLAST analysis (Altschul).