1039-o2 mutant for Exome-seq
B73 mutants were generated by gamma radiation of M0 seeds. Test line 1039-o2 was created by crossing a random B73 M0 plant (line 1039) to W64Aopaque2 (o2), self-pollinating the M1 and selecting for homozygous o2 seeds in the segregating M2 ears. M2 mutant seeds were germinated and DNA was extracted from leaf tissue of individual plants and compared with B73 wild type DNA (also extracted from a single plant) using exon capture DNA sequencing.