RNA-Seq of Mus musculus infected A. fumigatus Af293, day 3 : adult female kidney
Poly (A) containing mRNA was purified from 8 ug of total RNA of each sample using Sera-mag Magnetic Oligo (dT) Beads (Illumina RS-100-0801); mRNA molecules were fragmented using divalent cations under elevated temperature followed by first and second strand synthesis.  End repair was performed and deoxyadenonsine triphosphate (dATP) added to the 3' end of the DNA fragments. Next, adapters were ligated to cDNA fragments, cDNA template purified and finally the cDNA libraries were amplified via PCR. All steps were performed according to manufacturer’s instructions. Before sequencing, the quantity and quality of each cDNA library was assessed using a 2100 Bioanalyzer (Agilent Inc.)