White Leghorn chicken RNA-Seq: Sample WLM21heart
We obtained fertilized eggs of the White Leghorn chicken breed and kept them under           standard incubator conditions. The left gonad, heart, spleen and liver were collected at           embryonic day 19 and stored in RNAlater (Qiagen) until preparation. Library and           RNA-sequence samples were prepared by the Wellcome Trust Centre for Human Genetics           Facility at Oxford University using standard methods. Four samples per sex were prepared           for each of the four tissues, and each individual was barcoded so that individual           variation in coding sequence and expression level could be tracked. Samples were sequenced           using Illumina HiSeq.