Rice leaf RNA-Seq after light induction
The frozen leaf samples were homogenized with TissueLyser II (Qiagen, Hilden, Germany). Then, total RNA was extracted by using Maxwell 16 LEV Plant RNA Kit (Promega, Madison, USA) and Maxwell 16 Automated Purification System (Promega). Concentration of RNA were measured with Quant-iT RNA Assay Kit (Thermo Fisher Scientific, Hampton, USA). An automated liquid handling system (Freedom EVO 150; TECAN, Mannedorf, Switzerland) and a thermal cycler (ODTC 384; Inheco, Munich, Germany) were utilized for RNA-seq library preparation. Selective depression of abundant transcripts such as rRNAs was conducted as described in Nagano et al. (2015). Then, RNA-seq library was prepared as described in Ishikawa et al. (2017). Sequencing single-end of 50 bp using Hiseq 2500 (Illumina, Hayward, USA) was carried out by Macrogen Co.