Small RNA sequencing of AC7643 . Not stressed .
The procedures of smRNA library construction were following the instructions of the manufacture (Solexa) and previous report (Wang et al. 2009). Briefly, after separated from total RNA by 15% denaturing PAGE, adaptors were ligated to both 5’ and 3’ of size selected smRNAs (16~35nt). The resulting ligation products were gel purified by 10% denaturing PAGE and the product was used for further reverse transcription. cDNA generated were sequenced on an Illumina HiSeq 2000 system (Illumina, California USA).