WXS of Human: 70 years paraganglioma patient female
The sections from paraffin-embedded tissues were prepared on glass slides, and then stained with hematoxylin-eosin (H&E). Tumor areas were chosen in unstained sections according to the reference H&E slide. DNA was isolated using the High Pure FFPET DNA Isolation Kit (Roche, Switzerland). Library preparation for exome sequencing was carried out using the Nextera® Rapid Capture Exome Kit (Illumina, USA). Sequencing was performed using the NextSeq 500 System (Illumina, USA). At least 60 million paired-end reads of 75 nucleotides in length were obtained for each sample with 300x minimal coverage.