APERC_PB01_92
The extracted DNA was first sheared using a g-TUBE (Covaris, MA, USA) (target size of 20 kb) and then converted into SMRTbell template libraries according to the manufacturers protocol (Pacific Biosciences, CA, USA). Size selection was performed using BluePippin (Sage Science, MA, USA) to generate the library. Sequencing was performed using P6-C4 chemistry on the PacBio RS II instrument at the King Abdullah University of Science and Technology (KAUST) Bioscience Core Laboratory (BCL) with 360 mins movies.