Fib_Mac_TNF_Rep1
Total RNA was first extracted using RNeasy mini kit (Qiagen). Tru-seq (non-stranded and PolyA selected) sample preparation kits (Illumina) were then used to purify poly-A transcripts and generate libraries with multiplexed barcode adaptors. Paired end libraries were multiplexed, pooled and sequenced using the Paired End Clustering protocol with 51x2 cycles sequencing for 4 lanes on an Illumina HiSeq 2500. Sequencing was performed by the Weill Cornell Medical College Genomics Resources Core Facility.