Metagenome of SW Biofilm: SS 316L
Genomic DNA was isolated from the biofilm collected from SS202 material exposed to seawater for 2 months using Power water DNA kit. Variable regions (v1-v9) of 16S DNA were amplified using PCR and PCR amplicons were purified and the template dilution factor was determine using Bioanalyzer. DNA library was enriched and emulsion on the diluted construct was prepared and QC & QA were done after enrichment. Sequencing of DNA library was carried out on Ion Torrent by 318 chip and possible to create 160 million features in 200 bp sequencing chemistry that will give 80-150 bp mean read length. The total data was 7000 to 8000 MB and the sequence data file in FASTQ format could be obtained. The sequences were assembled and aligned to identify the bacterial organisms to species level using MG-RAST workflow.