GSM3073262: 30_NoH2O-H63-Seedling-6dat-3; Glycine max; RNA-Seq
After 9 d of treatment (pH 3.0 medium and control) or 6 d treatment (dehydration and control), seedlings were flash-frozen in liquid nitrogen, lyophilized to dryness, ground to a fine powder and stored at – 80˚C for extraction of metabolites and RNA. Tissue powder was freeze dried again for 1 h, then weighed prior to extractions. Lyophilized tissue was pulverized for 3 min using 5 mm stainless steel grinding balls in a MM400 mixer mill (Retsch, Newtown, CT, USA) at frequency of 30/s. Total RNAs were extracted the using the Spectrum Plant Total RNA Kit. The mRNA stranded library prep kit from KAPA Biosystems was used to build the libraries
GEO Accession
GSM3073262