GSM3475510: klp10a-KD rep1_mRNAseq; Drosophila melanogaster; RNA-Seq
300 pairs of testes were removed and Total RNAs were extracted by the Trizol method. For mRNA sequencing, 2.5 µg of total RNAs were treated with DNase I (Amplification Grade, ThermoFisher) and recovered by RNA Clean & Concentrator-25 columns (Zymo). DNase I treated RNAs were then treated with Ribo-Zero Gold rRNA Removal Kit (Illumina). Libraries were generated from rRNA depleted RNAs using TruSeq Stranded Total RNA Sample Prep Kit (Illumina).
GEO Accession
GSM3475510