GSM3141415: WT_12hpf; Danio rerio; RNA-Seq
Total RNAs were extracted from staged embryos using RNeasy Mini Kit (QIAGEN). 2μg of total RNAs from embryos were used for synthesis of the cDNA using a Library Preparation kit (DNAFORM). 5'caps of RNAs are biotinylated after reverse transcription. RNA-cDNA hybirds were then captured by streptavidin-conjugated magnetic beads. The cDNAs were released from RNAs and ligated with the 5′ linker, which includes the three nucleotide barcodes, followed by 3′ linker ligation. Second-strand synthesis was performed using the nAnT-iCAGE second primer, creating the final double-stranded DNA product. Cap analysis gene expression (CAGE) sequencing
GEO Accession
GSM3141415