GSM1924874: lnc-OPC knockdown repeat2; Mus musculus; RNA-Seq
Total RNA was extracted using the miRNeasy kit (Qiagen) under manufactor’s protocols. The quality was accessed by Bioanalyzer. Samples with high RNA integrity number (RIN) (>8) were used for library construction. 100ng total RNAs were used for each sequencing library. RNA-Seq was performed on the polyadenylated fraction of RNA isolated from differentiated cell samples. Two biological replicates were used. 100 ng total RNAs were used for each sequencing library. RNA samples were polyA selected and paired-end sequencing libraries were constructed using TruSeq RNA Sample Prep Kit as described in the TruSeq RNA Sample Preparation V2 Guide (Illumina).The samples were then sequenced using the Illumina HiSeq 2000 sequencer.
GEO Accession
GSM1924874