GSM1684593: Arabidopsis thaliana (Col-0, 11 days); Arabidopsis thaliana; OTHER
A. thaliana seeds were surface sterilized with 70% ethanol, then stratified at 4 degrees celsius for 2 days. Seeds were then sown on 1/2 Murashige and Skoog media containing 1% agar, and grown vertically for 11 days under short-day photocycles at 22 degrees celsius (8 hr light/ 16 hr dark) at a density of about 100 seeds per 10cm2 plate. Genomic DNA was prepared as manufacturer’s instruction. 5-10ug of gDNA was sheared to >10kb using g-tube (Covaris). The sheared DNA was treated with DNA damage repair mix followed by end repair and ligation of SMRT hairpin adapters using SMRTbell Template Preparation Reagent Kit (Pacific Biosciences). Fragments without adaptors are digested with exonuclease. Libraries were sequenced on Pacific Biosciences RSII sequencer using standard protocols.
GEO Accession
GSM1684593