GSM2218729: CI224_M replicate 1; Pseudomonas aeruginosa; RNA-Seq
Samples were lyophilized and lysed (5 x 1 minute with 2 minutes on ice between rounds) using a mixture of 0.1 mm, 0.5 mm and 1 mm beads in a Biospec Mini-Beadbeater-16 (Biospec Products, Bartlesville, OK). Samples were then re-suspended in TRI-Reagent (Zymo Research Corporation, Irvine, CA). RNA was extracted using Direct-zol RNA MiniPrep kits (Zymo Research Corporation, Irvine, CA) according to the manufacturer’s instructions. Directional RNAseq libraries were prepared with the TruSeq Stranded mRNA Library Prep Kit with unique TruSeq indexes.
GEO Accession
GSM2218729