MinION sequencing
Whole genome sequencing of the bacteriophage lambda genome. Read until has been applied to select reads whose start site maps to 10-15 kb or 35-40 kb of the genome. Read until is only applied to the even numbered channels, allowing odd numbered channels to act as a control for the library.
Lambda whole genome libraries were prepared without PCR amplification according to the standard protocol using version 5 library preparation kits (ONT, SEQ-MAP006) supplied by Oxford Nanopore Technologies (ONT). This protocol has been extensively described by Ip et al. Library was sequenced on a Mk1 minION using FLO-MAP103 flow cells.