GSM2649531: shLacZ18-H3K4me3; Homo sapiens; ChIP-Seq
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Sequencing libraries were prepared from ChIP DNA by the MUSC Genomics Core Facility with Rubicon ThruPLEX library preparation kits and sequenced as 35 bp single-end reads on an Illumina HiSeq 2500. Libraries for three biological replicates were prepared and sequenced for each sample type.
GEO Accession
GSM2649531