GSM3380240: replicate 2 of SK11; Lactococcus lactis; RNA-Seq
RNA isolation was performed using routine procedures, as described previously (Bron et al., 2012) with minor adjustments. Aliquots of 5 ml of culture were centrifuged at 4000 × g for 3 minutes at 2°C and cells were resuspended in 0.5 ml cold TE buffer. To this suspension, 500 µl 1:1 phenol/chloroform, 30 µl 10% SDS, 30 µl 3M sodium acetate pH 5.2 and 500 mg 0.1 mm zirconia beads (Biospec Products, Inc., Bartlesville, USA) was added in a 2 ml screw-cap tube and samples were frozen in liquid nitrogen and stored at -80°C. cDNA synthesis and sequencing with an Ion Proton system using an Ion PI-chip (Life Technologies) was performed by PrimBio Research Institute (Exton, USA).
GEO Accession
GSM3380240