GSM2991372: BRG1 binding in resistant clone 3 of TOV21G cell line; Homo sapiens; ChIP-Seq
Flag (Sigma, F1804) antibody was used on TOV21G and resistant endogenously Flag-tagged BRG1 cells. Chromatin were incubated overnight at 4°C and protein A+G Dynabeads were added to the reaction for another 1.5 hour. Magnetic beads were washed and chromatin was eluted and reversed. Chromatin was then treated with proteinase K and purified with Gel extraction kit (Qiagen, cat. no. 28706). ChIP DNA was used for ChIP-seq. For CHIP-seq NEBNext Ultra DNA Library Prep Kit (NEB, E7645) was used to prepare sequencing library
GEO Accession
GSM2991372