Transcriptome sequencing originated from the hypodermis and the Y organ of the crustacean Astacus leptodactylus
RNA was extracted from the gills of vitellogenic females of Pontastacus leptodactylus two hours after injection of D- or L-Crustacean hyperglycemic hormone or carrier injection as control. The Trireagent extraction kit of Sigma, USA was used for the extraction. The extracted RNAs were further purified using Qiagen RNeasy kit. The various extractions were equally mixed and a total of 5 mg was sent to sequencing after quality examination using bioanalyser (Agilent 2100).