454 GS FLX sequencing; 16S_rRNA_mouse_colon_experiment
Interfold and digesta regions microbes were collected from the ascending colon of wild-type mice by laser capture microdissection (LCM). DNA was extracted from the LCM-procured material and used for tag-encoded pyrosequencing analysis of 16S rRNA genes using well-validated primers (27F and 338R), tags and protocols. PCR amplifications were performed in quadruplicate, and products were pooled. Purified PCR products were subjected to high-throughput pyrosequencing using the GS-FLX Titanium platform. 4 separate barcodes used for each of the 2 samples.