GSM2054809: RNASeq_WT_Basal; Mus musculus; RNA-Seq
RNA-seq: Poly(A) RNA were purified twice with Trizol and RNeasy® Plus Mini Kit (Qiagen). cDNA was synthesized using SuperScript II (Invitrogen) and TruSeq RNA Sample Preparation Kit (Illumina). Mammary epithelial cells (MECs) were isolated from 12-week-old virgin mice using a standard protocol and non-epithelial cells were removed using the EasySepTM Mouse Mammary Stem Cell Enrichment Kit (STEMCELL TECHNOLOGY, #19757). MECs were stained with biotinylated anti-CD24-fluorescein isothiocyanate (FITC, BD Biosciences), anti-CD49f-R-phycoerythrin (R-PE, BD Biosciences) and 7-amino-actinomycin D (7AAD, BD Biosciences) and were sorted to basal (CD24+CD49fhi) and luminal (CD24hiCD49flo) cell populations after FACS analysis was performed as previously described (Yoo, 2014). Libraries were prepared for sequencing using standard Illumina protocols.
GEO Accession
GSM2054809