Pooled sequencing of cosmid clones from metagenomic libraries
Metagenomic libraries were constructed by cloning fragments of environmental DNA into the cosmid vector pJC8 (KC149513.1). Multiple libraries were constructed, each of which has a BioSample accession. Various function-based screens of the libraries were carried out, and 92 clones were selected for Illumina sequencing using a pooled strategy (to compare to a barcoded strategy).