GSM3832983: FST 3; Mus musculus; RNA-Seq
After AS or no stress-exposure, Mice were killed by cervical dislocation and rapidly decapitated to extract the hippocampi for translating ribosome affinity purification (TRAP) protocol of CA3 pyramidal neurons. TRAP was performed as described in Gray et al., 2018 (doi: 10.1038/mp.2016.219). A total of 200ng of RNA per group was prepared for sequencing by The Rockefeller University Genomics Core Facility using the Tru-Seq RNA-Sample Preparation Kit v2 (Illumina, USA). Samples were sequenced on Illumina HiSeq 2500 in a single lane to obtain 100-bp single-end reads at an approximate sequencing depth of 25–35 million reads per sample. The libraries were barcoded to allow for multiplexing within a cell lane. Three biological replicates were used per experimental group, comprising of RNA pooled from 5-6 mice each. At the time of sequencing, experimental groups consisted of three replicates per condition
GEO Accession
GSM3832983