Coastal Pacific Drifting ESP 9/16/2010 2pm 0.22-5um cDNA
Library preparation followed the Titanium Rapid Library Preparation protocol. In order to improve the retention of smaller cDNA molecules, adaptor-ligated libraries were not diluted prior to size selection with AMPure XP beads. Libraries were quantified using the Titanium Slingshot kit (Fluidigm) and added to emulsion PCR reactions at 0.1 molecules per bead. Sequencing and quality control were followed manufacturer recommendations.