Bacterial diversity of Halichondria panicea before ex situ cultivation
V3-V4 region of the 16S rRNA gene was amplified using the universal bacterial primer pair S-D-Bact-0341-b-S-17 (5-CCTACGGGNGGCWGCAG-3) / S-D-Bact-0785-a-A-21 (5-GACTACHVGGGTATCTAATCC-3). Libraries were multiplexed using Nextera XT v2 indices and sequenced on a MiSeq System with v3 chemistry and 2 x 300