GSM2810603: Berberine; Bacillus subtilis subsp. subtilis str. 168; RNA-Seq
One-hour alkaloid and DMSO treated cells were placed in RNAprotect Bacteria Reagent (Qiagen, Germany) and incubated for 5 minutes at room temperature. Cell suspensions were centrifuged at 5,000xg for 10 minutes. Total RNA isolation was achieved using RNeasy Mini Kit (Qiagen, Germany) according to manufacturer's protocol with mechanical disruption at 6 m/s for 40 seconds (FastPrep Instrument, MP Biomedicals, USA). RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM2810603