Ammonia amended soil from 56-days aerobic dynamic: day 0
This paired-end SRA file contains the V1-V3 16S rRNA raw sequences as a multiplexed library from the 18 ammonia amended soil samples for Day 0 of the 56-days aerobic dynamic. The paired-end fastq files must be fed into the split_libraries_fastq.py script to demultiplex each Sample by its 16-bp unique barcode. The mapping file to be used is the following; where '$' must be changed to tabulation ('\t') and '@' to a new line ('\n'): SampleID$BarcodeSequence$LinkerPrimerSequence$ReversePrimer$soil_use$dinamic$Practice$Fertilizer$Day$Treatment$Pract.Day$Pract.Fertil.Day$Day.Fertilizer$Soil$OH$Description@DNA01$TAAGGCGATAGATCGC$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$amended$CT$0$0$CT0$CT.0$CT.0.0$D0.0$111$1$Soil.NH4.1@DNA02$TAAGGCGACTCTCTAT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$amended$CT$0$0$CT0$CT.0$CT.0.0$D0.0$211$2$Soil.NH4.2@DNA03$TAAGGCGATATCCTCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$amended$CT$0$0$CT0$CT.0$CT.0.0$D0.0$311$3$Soil.NH4.3@DNA04$TAAGGCGAAGAGTAGA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$amended$CT$300$0$CT300$CT.0$CT.300.0$D0.300$115$4$Soil.NH4.4@DNA05$TAAGGCGAGTAAGGAG$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$amended$CT$300$0$CT300$CT.0$CT.300.0$D0.300$215$5$Soil.NH4.5@DNA06$TAAGGCGAACTGCATA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$amended$CT$300$0$CT300$CT.0$CT.300.0$D0.300$315$6$Soil.NH4.6@DNA07$TAAGGCGAAAGGAGTA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBB$0$0$PBB0$PBB.0$PBB.0.0$D0.0$121$7$Soil.NH4.7@DNA08$TAAGGCGACTAAGCCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBB$0$0$PBB0$PBB.0$PBB.0.0$D0.0$221$8$Soil.NH4.8@DNA09$CGTACTAGTAGATCGC$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBB$0$0$PBB0$PBB.0$PBB.0.0$D0.0$321$9$Soil.NH4.9@DNA10$CGTACTAGCTCTCTAT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBB$300$0$PBB300$PBB.0$PBB.300.0$D0.300$125$10$Soil.NH4.10@DNA11$CGTACTAGTATCCTCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBB$300$0$PBB300$PBB.0$PBB.300.0$D0.300$225$11$Soil.NH4.11@DNA12$CGTACTAGAGAGTAGA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBB$300$0$PBB300$PBB.0$PBB.300.0$D0.300$325$12$Soil.NH4.12@DNA13$CGTACTAGGTAAGGAG$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBC$0$0$PBC0$PBC.0$PBC.0.0$D0.0$151$13$Soil.NH4.13@DNA14$CGTACTAGACTGCATA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBC$0$0$PBC0$PBC.0$PBC.0.0$D0.0$251$14$Soil.NH4.14@DNA15$CGTACTAGAAGGAGTA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBC$0$0$PBC0$PBC.0$PBC.0.0$D0.0$351$15$Soil.NH4.15@DNA16$CGTACTAGCTAAGCCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBC$300$0$PBC300$PBC.0$PBC.300.0$D0.300$155$16$Soil.NH4.16@DNA17$TCCTGAGCTAGATCGC$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBC$300$0$PBC300$PBC.0$PBC.300.0$D0.300$255$25$Soil.NH4.17@DNA18$TCCTGAGCCTCTCTAT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBC$300$0$PBC300$PBC.0$PBC.300.0$D0.300$355$26$Soil.NH4.18