GSM3140706: KELLY RNA-seq; Homo sapiens; RNA-Seq
For NONO KD samples and total RNA samples (KELLY, BEC2 and SK-N-AS, cells were lysed using Nucleozol ™? by Machery-Nagel. For PAR-CLIP samples, after PAR-CLIP protocol was conducted (including immmunoprecipitation, phosphorus-32 treatment, SDS-PAGE electrophoresis and proteinase K treatment), samples were extracted using the Qiagen™? miRNeasy RNA extraction kit. RNA extracted from PAR-CLIP was converted into cDNA using the TruSeq small RNA Kit v2 (illumina). RNA extracted from other samples was converted to cDNA with the TruSeq Stranded Total RNA Library Prep Kit (illumina) and rRNA depleted with the Ribo-Zero-Gold kit (illumina). All samples were sequenced on illumina Hiseq 2000 to obtain 50bp single end reads.
GEO Accession
GSM3140706