Exome sequencing of adult human from Siberia
Samples were individually bartagged and then pooled into 3 groups of 7. A library was created for each pooled group and the 3 groups were then each sequenced on a single Illumina Hiseq 2000 lane. Data were then demultiplexed and individual FASTQ files were created. FastQC was used for quality control. Adapters were trimmed with trimmomatic. The reads from each individual were then aligned to hg19 using BWA. Picard and GATK were used for removing PCR duplicates, variant quality score recalibration and realignment around indels. The pipieline followed the standard proceedures for clinical exome sequencing at the University of Arizona Genomics Core.