GSM3380036: 13CHamp; aquifer metagenome; RNA-Seq
RNA was extracted with the Powersoil RNA isolation kit from QIAgen. Heavy (representing microbes that metabolized toluene) and light (microbes that did not metabolize toluene) RNA was separated by isopycnic centrifugation. Additionally, subsamples of RNA from Heavy and Light fractions underwent linear amplification with the MessageAmp II Bacteria kit from Thermofisher to compare the effects of amplification against unamplified samples. Libraries were produced according to manufacturer instruction using the Illumina TruSeq® Stranded mRNA HT kit.
GEO Accession
GSM3380036