RNA-seq of homo sapiens: SUM185PE triple-negative breast cancer cell line
Total RNA was isolated from SUM185PE cells using Rneasy (Qiagen). RNA quality was assessed by NanoDrop (Thermo) and Bioanalyzer (Agilent). Two ľg of total RNA were used for the TruSeq Stranded Total RNA Library Prep Kit (Illumina). Libraries were quantified by Qubit (Life Technologies) and qPCR, and library size and quality were assessed by Bioanalyzer. Reads were de-multiplexed and trimmed using SeqPrep.