RNA-seq of downy mildew resistant cucumber: 24hpi bioreplicate 2
The downy mildew-resistant cucumberŁ¨PI 197088ŁŠleaf samples were collected at 0, 6, and 24 h after Pseudoperonospora cubensis inoculation, and denoted as 0hpi, 6hpi, and 24hpi, respectively. Sample 24 hpi were immediately taken within 24h after inoculation. There were two biological replicates per time point. Total RNA was isolated from three time points and two biological replicates independently. mRNA was extracted using Oligo (dT) magnetic beads and fragmented as a template for complementary DNA (cDNA) synthesis. The purified cDNA fragments with sequencing adapters were sequenced using Illumina HiSeqTM 2000 to generate 90 bp paired end reads.