Plant sample from Hordeum vulgare
For each short-insert size library, about 5 μg of DNA was fragmented, end-repaired, size selected at 250,300 and 500 base pairs (bp) on agarose gels, ligated to Illumina PCR free paired-end adapters. Biotinylated fragments were purified on magnetic beads (Invitrogen) and used to construct libraries.