GSM1923357: mitoRNAseq; Saccharomyces cerevisiae; RNA-Seq
sample processing: RNA was isolated from purified mitochondria by Trizol, randomly fragmented by alkaline hydrolysis, and 36-42 nt fragments were gel purified. Gel-purified RNA was treated with PNK and ligated to a pre-adenylated adapter using T4 RNA ligase 2, K227Q, truncated. Superscript III was used for reverse transcription and circ ligase for circularization. Illumina adapters were introduced by 10-14 cycles of PCR.
GEO Accession
GSM1923357