RNA-Seq of Aspergillus oryzae RD2: mycelium expansion
Sample was extracted at about 28h of koji fermentation.  RNA was then extracted using the RNeasy Micro Kit (Qiagen). Integrity of the RNA extracted was evaluated using gel electrophoresis and a NanoDrop Lite Spectrophotometer (Thermo Fisher Scientific). Transcriptomes were then sequenced on an Illumina HiSeq 2000 sequencing system at BGI Genomics.