GSM2219057: Hypoxia nongrowing, replicate 2; Mycobacterium tuberculosis H37Rv; RNA-Seq
Regrowth cultures were harvested by centrifugation after treatment with RNAprotect Bacteria Agents (Qiagen) for 15 minutes to stabilize RNA, and RNA was extracted with Trizol. rRNAs were first removed from total RNAs using the Ribo-zero rRNA removal kit (Epicenter, Applied Systems); after mRNA fragmentation, first strand cDNA synthesis was carried out using Super Script II (Invitrogen) reverse transcription system; after the second strand cDNA synthesis, the double strand (ds) cDNAs were repaired and their 3' end adenylated for adapter ligation; after ligating multiple indexing adapters, the dsDNA library was enriched by PCR and validated; the qualified library was amplified and sequenced with 90 bp pair-end reads.
GEO Accession
GSM2219057