Selecting superior de novo transcriptome assemblies: lessons learned by leveraging the best plant genome
20ug of high quality (28s/18s ratio: ≥1.7; RIN ≥8; A260/A280: ≥1.8), DNase treated RNA from each biological replicate was thoroughly mixed. Pooled samples were provided to Chris Pires at the University of Missouri, Columbia for cDNA synthesis (Evrogen Mint dscDNA synthesis kit), normalization (Evrogen Trimmer Kit cDNA normalization kit) and a sequencing on a single lane following a paired-end (120x120bp) sequencing cycle protocol.