Genome sequencing of Pectobacterium carotovorum strain SCC1
Genomic DNA was extracted from overnight grown bacteria using phenol-chloroform purification followed by ethanol precipitation. DNA was fragmented into approximate size of 800 bp using Nebulizer (Roche) followed by standard fragment 454 library with the GS FLX series reagents. Genomic DNA was sequenced using 454 (454 Life Sciences/Roche).