Mata
Environmental DNA was extracted from each soil sample using the FastDNA® SPIN Kit for Soil (MP Biomedicals, Santa Ana, CA, USA) and the FastPrep® sample preparation system (MP Biomedicals) according to the manufacturer’s instructions.  DNA samples (1.0 mg per sample) were prepared for sequencing by nebulization, followed by tagging using the GS FLX Titanium Rapid Library MID Adapters Kit (454 Life Sciences).