ES E14 RNAseq (with spike-ins) rep2
To obtain more precise measurements of transcription, RNA spike-ins were used.  First, total RNA from 106 cells was isolated by Trizol extraction. Second, 1μl of 1/10 dilution of Ambion’s ERCC RNA Spike-in Mix (catalog number 4456740) was added to total RNA. mRNA was then isolated and the standard RNA-Seq library preparation was performed following Illumina’s RNA-Seq protocol v2.