Trilobodrilus axi Adult transcriptome
The library was prepared with double indices following the protocol described by Meyer and Kircher (2010, Illumina sequencing library preparation for highly multiplexed target capture and sequencing. Cold Spring Harbor protocols 2010: pdb prot5448) and Kircher et al (2011, Double indexing overcomes inaccuracies in multiplex sequencing on the Illumina platform. Nucleic Acids Res 2011: 1–8) and as described by Hering et al (2012, Opsins in Onychophora (velvet worms) suggest a single origin and subsequent diversification of visual pigments in arthropods. Mol Biol Evol 29: 3451-3458) using total RNA. The libraries were sequenced at the Max Planck Institute of Evolutionary Anthropology (Leipzig), using an Illumina Genome Analyzer IIx (GAIIx) with 96 cycles paired end.