Male gonad transcriptome for the rock bream
The fully matured testis at stage V were dissected from wild captured rock bream and immediately stored in RNAlater (Qiagen, Hilden, Germany) and then stored at -80oC prior to RNA extraction. The gonad maturation of the rock bream was check by histological section. Sperm from the male individual can be easily striped and the lobular was filled with spermatozoa under microscope, which suggest the male is mature at stage V.