GSM3379771: MEG3 TFR1 rep1; Homo sapiens; OTHER
RNA-DNA complexes were bound to MyOne Streptavidin C1 Dynabeads (Invitrogen) and washed three times in a buffer containing 10 mM Tris-HCl (pH: 7.4), 50 mM KCl, 5 mM MgCl2, 0.05% Tween-20. RNA-associated DNA was eluted with 25 ng/μl RNase A and 5 mU/μl of Rnase I for 30 min at 37 ° C and recovered by phenol/chloroform extraction. DNA libraries were prepared from two independent experiments using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) and NEBNext Multiplex Oligos for Illumina (NEB) and sequenced on a NexSeq500 Illumina platform.
GEO Accession
GSM3379771