GSM2052295: E13_5_PGC_Tet1wt_mCG Met-seq; Mus musculus; Bisulfite-Seq
PGCs were lysed in lysis buffer (10mM Tris-Hcl PH 7.5, 10mM NaCl, 0.5% NP-40, protease K) for 3h at 55°C and heat-inactivated for 1h at 72°C. Lamda DNA was added as control to calculate bisulfite conversion rate. The DNA mixture was digested by dsDNA fragmentase (NEB) and followed by bisulfite conversion using EpiTect Fast Bisulfite Conversion Kit (Qiagen). The bisulfite single strand DNA was subject to TELP, described in "TELP, a sensitive and versatile library construction method for next-generation sequencing" by Peng X, Wu J, Brunmeir R, Kim SY, Zhang Q, Ding C, Han W, Xie W, Xu F., published in Nucleic Acids Res. 2015 Mar 31;43(6):e35. doi: 10.1093/nar/gku818. Epub 2014 Sep 15.
GEO Accession
GSM2052295