GSM1252086: col RNA-seq; Arabidopsis thaliana; RNA-Seq
RNA was extracted with Trizol reagent from 14-d Arabidopsis leaves, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser. The extracted RNA was sent to BGI (Shenzhen, China) for RNA-seq library preparation and whole transcriptome sequencing. Briefly, The total RNA was treated with DNaseI and then subjected for mRNA enrichment. The derived mRNA was used as the template for first strand cDNA synthesis by using oligo dT primer. The adaptor was added to the 5’ end of the first strand cDNA and then the second strand cDNA was synthesized by using the primer pairing to the adaptor. The double strand cDNA was sonicated into 300-500 bp fragments for pair-ended sequencing with HiSeq 2000.
GEO Accession
GSM1252086