GSM1680208: JSC.6190 Johnson Space Center study; rat gut metagenome; OTHER
Total DNA was isolated from fecal samples using Qiagen's protocol Initial amplification of the V1-V2 region of the bacterial 16S rDNA was performed on total DNA isolated from fecal samples. Each sample was individually gel purified using the E-Gel Electrophoresis System. To ensure equal representation of each sample in the sequencing run, each barcoded sample was standardized by calculating equimolar amounts prior to pooling. Pooled samples of the 16S rDNA multiplexed amplicons were sequenced on a Roche 454 Genome Sequencer FLX Titanium instrument using the GS FLX Titanium XLR70 sequencing reagents and protocols.
GEO Accession
GSM1680208