active bacteria in coastal lagoon sediment
Sediment was sampled in a coastal lagoon, Magdalen Islands over 12 dates from 30 May 2009 to 23 August 2010. The top 2cm surface layer was used for RNA extraction.  RNA was converted to cDNA and the 16S rRNA was amplified by PCR.  The PCR products were used as template for the 454 pyrosequencing procedure.  The sequences were analysed via a pipeline using MOTHUR.