RNAseq of isolates from 01_ZC3H18 knock-down cells
HeLa cells were transfected with siRNAs targeting the gene noted in the library title. After verification of knock-down efficiency by western blots total RNA was depleted of rRNA (RiboMinus) and RNA-seq libraries were prepared using the ScriptSeq v2 kit (EpiCentre). Libraries were sequenced 100 nt paired end on an Illumina HiSeq 2000 platform.