Trypanosoma cruzi G1 enriched epimastigotes transcriptome
Trypanosoma cruzi epimastigotes axenic cultures were synchronized by Hydroxiurea incubation and release experiments.  G1-phase enriched epimastigotes were harvested at 0 hours post-HU. Total RNA was obtained with TRIzol reagent. Poly-A containing transcripts were captured by oligo-dT affinity. 50 bp single-ended RNA-seq libraries were constructed using established protocols. Deep sequencing was performed on a Illumina HiSeq 2000.