GSM4548445: WDR43-8h-ATACseq-1; Mus musculus; ATAC-seq
ATAC-seq was performed as previously described (Buenrostro et al., 2013) with modifications. Briefly, 1 μl diluted cells (~1,500 cells) were lysed in 6 μl lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2 and 0.5% NP-40) for 10 minutes on ice. Lysed nuclei were then incubated with the Tn5 transposase and tagmentation buffer (Vazyme Biotech) at 37 °C for 30 minutes. After the tagmentation step, stop buffer was added and PCR was performed to amplify the library for 12 cycles using the following PCR conditions: 72 °C for 3 minutes; 98 °C for 30 seconds; thermocycling at 98 °C for 15 seconds, 60 °C for 30 seconds and 72 °C for 3 minutes; then 72 °C for 5 minutes. After the PCR reaction, libraries were purified with 1.7× AMPure (Beckman) beads. Libraries were prepared by TruePrep DNA Library Prep Kit V7 for Illumina® according to the standard protocols provided by the manufacturer.
GEO Accession
GSM4548445