GSM1679877: J-61; Mus musculus; Bisulfite-Seq
generation protocol: One million EGCs or ESCs were combined with one million MEFs and fused with PEG-1500 (Roche) according to the manufacturer's directions. Selection was initiated 24 hr post-fusion with puromycin (2 mg/mL) and neomycin (300 mg/mL). Cell-cycle analysis was performed on a FACSCalibur (BD) with propidium iodide; signal area was used as a measure of DNA content. Genomic DNA was collected using phenol/chloroform extraction mRRBS libraries were prepared as previously described (Boyle et al. 2012). Briefly, genomic sequences were digested using the MspI restriction enzyme, which cuts at the sequence CCGG. Digested fragments were subjected to bisulfite treatment which converts unmethylated cytosine residues to uracil
GEO Accession
GSM1679877