GSM4116597: WM278 shCDR1as_IGF2BP3_RIP_2; Homo sapiens; RIP-Seq
RIP assay was performed with control IgG or IGF2BP3-specific antibody (MBL International) using RIP Assay Kit (MBL International) following manufacturer supplied protocol, following the high-salt wash method. Cell suspensions were thawed briefly on ice prior to lysis and RIP assay. Input RNA samples and eluted IGF2BP3 RIP RNA samples were used in standard RNA-seq library preparations. Total RNA was depleted of rRNA by Ribo-zero gold. Stranded libraries were prepared by Illumina ScriptSeq Complete Gold/TruSeq Stranded following manufacturer's recommendations.
GEO Accession
GSM4116597