GSM3832909: 100Pa_rep2; Homo sapiens; ATAC-seq
Cells were extracted from matrices by incubating gels in ice cold 50 mM EDTA in PBS for 10 minutes with pipette mixing. The suspensions were centrifuged at 500 g at 4oC for 10 mins to pellet cells. The pellets were incubated in 1 ml of 0.25% trypsin/2.21 mM EDTA at 37oC for 5 minutes to digest any remaining rBM, then centrifuged at 500 g for 10 mins again. Libraries were prepared using Illumina Nextera Tn5 Transposase (FC-121-1030)
GEO Accession
GSM3832909