GSM3535551: MM.1S 10 nM E7107 mRNA replicate 3; Homo sapiens; RNA-Seq
Total RNA was extracted by standard RNA extraction kit (RNeasy, Qiagen) and cleaned up with RNA clean & concentrator columns (Zymo).  For all except the timecourse samples, polyA-mRNA was enriched from total RNA with polyA-binding magnetic bead protocol (NEB). RNA libraries were prepared for sequencing using standard Illumina protocols and Hyper Prep RNA sequencing kit (Kapa, with RiboErase for timecourse samples to remove abundant rRNA).  Protocol aimed at generating 200-300 bp fragments.  Reverse-transcribed cDNA was ligated to TruSeq barcode adapters for Illumina platform.  200-400 bp cDNA libraries were selected by PAGE extraction and purification, or AMPure bead size selection in the case of SRSF1 overexpression samples.  Four samples were multiplexed in a single lane for sequencing for the timecourse samples, seven samples were multiplexed for the single timepoint samples, and 9 samples were multiplexed for the SRSF1 overexpression samples.
GEO Accession
GSM3535551