Prp16_3rDbr1
Prp16 was immunoprecipitated from yeast cells, which is expected to pull down spliceosomal complexes and lariats. Then, RNAs were extracted from these IP fractions and the treated with XRN1/CIP/RNase R to remove linear RNAs. Then, the rDbr1 treatment was carried out to debranched the lariats and the 3'ends of resulting RNAs were sequenced to reveal the branch point.