Microbial diversity for La Estrella (le) and La Negrita (ln) sites in no-tillage (A) and uncropped (N) soil types
DNA was isolated from 10g of soil using the PowerMaxSoil DNA Isolation Kit (MO BIO Laboratories, Inc, USA). The hyper variable V4 region of the 16S rRNA gene was amplified using the following universal primers containing the Roche 454 sequencing A and B adaptors and a 10 nucleotide "multiple identifier" (MID) to sort samples: 520F (5' CGTATCGCCTCCCTCGCGCCATCAGACGAGTGCGTAYTGGGYDTAAAGNG 3', where ACGAGTGCGT is an example, different MIDS for each sample were used) and 802R (5' CTATGCGCCTTGCCAGCCCGCTCAGTACCRGGGTHTCTAATCC, 5' CTATGCGCCTTGCCAGCCCGCTCAGTACCAGAGTATCTAATTC, 5' CTATGCGCCTTGCCAGCCCGCTCAGCTACDSRGGTMTCTAATC, 5' CTATGCGCCTTGCCAGCCCGCTCAGTACNVGGGTATCTAATCC) (modified from RDP's Pyrosequencing Pipeline: http://pyro.cme.msu.edu/pyro/help). The samples were multiplexed in the following way: Region 1: leA (ACGAGTGCGT); leN (ACGCTCGACA); lnA (AGACGCACTC); lnN (AGCACTGTAG).