GSM3230512: Isotype PD1hi rep 3; Mus musculus; ATAC-seq
CD8+ T cells from mouse spleen were sorted by flow cytometry for naive (Lineage (CD19, CD11b, NK1.1)negative, TCRbeta+, CD8+ CD127+, CD44-), memory (Lineage (CD19, CD11b, NK1.1)negative, TCRbeta+, CD8+ CD127+, CD44+), effector PD-1int (Lineage (CD19, CD11b, NK1.1)negative, TCRbeta+, CD8+ CD127-, CD44+, PD1int)  and effector PD-1hi (Lineage (CD19, CD11b, NK1.1)negative, TCRbeta+, CD8+ CD127-, CD44+, PD1hi) CD8+ T cells in PBS + 5%FCS. Sorted cells were incubated with Tn5 transposase (Nextera DNA Library Preparation Kit; Illumina) at 37°C for 30 min in the presence of 0.125 % Igepal CA-630 (Sigma-Aldrich). Tn5 transposase was stopped with EDTA (final conc. 9 mM; Invitrogen). The ATAC-seq libraries were purified with Ampure XP beads (Beckman Coulter), after the barcodes were added by 13-14 PCR cycles.
GEO Accession
GSM3230512