16S rDNA fragment gene of microbial community from 6th planktonic phase of membrane biofilm reactor used to culture Candidatus liberibacter asiaticus
The V1-V3 variable region of the 16S rDNA gene was amplified using standard PCR from the extracted gDNA with primers 27F (GAGTTTGATCMTGGCTCAG) and 515R (TTACCGCGGCTGCTGGCAC). After this PCR amplification, the amplicons were purified (Qiagen PCR purification kit) and quantified (Qubit). Barcoded amplicons were pooled and sequenced using a PacBio-RSII sequencer (WSU genomics core, USA).