GSM1682281: ADM_Methylation; Homo sapiens; Bisulfite-Seq
For RRBS, Genomic DNA was digested with MspI enzymes (NEB) and blunt-ended. Then dA was added, followed by methylated-adapter ligation. To obtain DNA fractions of 40-120 bp and 120-220 bp ranges of MspIdigested products, two ranges of 160-240 bp and 240-340 bp adapter-ligated fractions were excised from a 2% agarose gel, respectively. Bisulfite conversion was conducted using a ZYMO EZ DNA Methylation-Gold Kit™ (ZYMO), following the manufacturer’s instructions. The final libraries were generated by PCR amplification using JumpStart™ Taq DNA Polymerase (Sigma). RRBS libraries were analyzed by an Agilent 2100 Bioanalyzer (Agilent Technologies). Total DNA and RNA was isolated.
GEO Accession
GSM1682281