16S Amplicons of marine sediments: Gulf of Mexico
Metagenomic DNA was extracted using a MoBio Powersoil DNA extraction kit. The V4 region of the 16S gene was amplified using the Earth Microbiome Project primerset (515F/806R) with modifications to allow barcoding with the Fluidigm Access Array system. Paired end sequences were paired using pear v.0.9.6 with default parameters.