miRNA-seq of 293FT cell line: let-7b transfection 32h
Total RNAs were isolated using Trizol and fragments between 18-29nt were size-selected from gel. RNAs were ligated to a 5ĄŻ and a 3ĄŻ adapter sequentially, reverse transcribed into cDNA and PCR amplified; the 140_160 bp. DNA fragments between 140-160nt were purified from the gel for sequencing.