HGM
Ion Proton shotgun sequencing   for library preparation clean genomic DNA (aprox. 1000 ng) were fragmented by 200-300 bp using Covaris S220 System (Covaris, Woburn, Massachusetts, USA). After that, Ion Xpress  Plus Fragment Library Kit (Life Technologies) was employed for barcode shotgun-library sample preparation. Emulsion PCR was performed using the One Touch system (Life Technologies). Beads were prepared using the One Touch 2 and Template Kit v2 Sequencing was performed by using Ion Proton 200 sequencing kit v2 on the P1 Ion chip. Raw data has been uploaded to NCBI SRA database