High-throughput amplicon analysis of protist-associated bacteria from Reticulitermes flavipes
Sequencing libraries were generated using barcoded primers to amplify the V4 region of bacterial 16S rRNA genes. The template for each PCR creation was a single protist cell isolated from a hindgut of Reticulitermes flavipes. Amplicons were gel-purified and sequenced on an Illumina MiSeq.