GSM2651146: UVB total, rep2; Homo sapiens; RNA-Seq
RNA was isolated using Trizol followed by phenol/chloroform extraction. The concentration and quality of total RNA samples were first assessed using Agilent 2100 Bioanalyzer. A RIN (RNA Integrity Number) of five or higher was required to pass the quality control. The samples are first spiked-in with ERCC Mix 1 to no treatment and Mix 2 to all treated. Then five hundred nanograms of RNA per sample were used to prepared dual-indexed strand-specific cDNA library using TruSeq Stranded mRNA Library PrepKit (Illumina). The resulting libraries were assessed for its quantity and size distribution using Qubit and Agilent 2100 Bioanalyzer. One and a half pico molar pooled libraries were sequenced with 2x75bp paired-end configuration on NextSeq500 (Illumina) using NextSeq 500/550 High Output Kit. A Phred quality score (Qscore) was used to measure the quality of sequencing. More than 90% of the sequencing reads reached Q30 (99.9% base call accuracy).
GEO Accession
GSM2651146