GSM4547587: 5_DPI; Mus musculus; RNA-Seq
GF+ SCs cells were isolated from muscle at the indicated timepoints following a published protocol (Liu et al., 2015). Cells were sorted using a live/dead stain to exclude debris or dead cells and GFP+ cells were subsequently selected and sorted for scRNA-seq libraries were prepared following 10X Genomics protocol for v3 3' single-cell sequencing and samples were prepared for high throughput sequencing following Illumina's specifications. Prior to sequencing all samples were evaluated using an Agilent Bioanalyzer high sensitivity chip single-cell RNA sequencing
GEO Accession
GSM4547587