GSM4547643: M13_Ctrl_DMSO_3_RRBS; Homo sapiens; Bisulfite-Seq
DNA was extracted using NucleoSpin Tissue kit (Macherey-Nagel). The following QC and library construction steps wer performed at Novogene. Agarose Gel Electrophoresis was run to test DNA degradation and potential RNA contamination. Qubit 2.0 was used to quantify DNA concentration.  After the DNA sample QC, positive control DNAs were added into the samples, and then they were fragmented into 200-400 bp using Covaris S220. Next, terminal repairing, A-ligation, and methylation sequencing adapters ligation were performed to the obtained DNA fragments. The final DNA library was obtained after bisulfite treatment (EZ DNA Methylation Gold Kit, Zymo Research), size selection, and PCR amplification steps. Library concentration was firstly quantified by Qubit 2.0. Then, the libraries were diluted to 1ng/µl before checking insert size on Agilent 2100 and quantified with higher accuracy by Q-PCR (effective concentration of library >2nM). After passing the library QC, different libraries were be pooled together and then fed into Illumina devices (Illumina Novaseq 6000) according to effective concentration and expected data volume. The sequencing strategy was paired-end sequencing 150 bp.
GEO Accession
GSM4547643