Analysis of porcine adipose tissue transcriptome reveals differences in de novo fatty acid synthesis in pigs with divergent muscle fatty acid composition
From the six selected animals, total RNA was isolated from backfat using the RiboPureTM Isolation of High Quality Total RNA (Ambion*, Austin, TX) following the manufacturer’s recommendations. RNA was quantified using the NanoDrop ND-1000 spectrophotometer (NanoDrop products, Wilmington, USA) and checked for purity and integrity in a Bioanalyzer-2100 (Agilent Technologies, Inc., Santa Clara CA, USA). Sequencing libraries were generated using Illumina mRNA-Seq following manufacturer’s instructions and six index codes were added to attribute sequences to each animal. Libraries were sequenced, in CNAG (Centro Nacional de An*lisis Gen*mico), on an Illumina HiSeq2000 instrument (Illumina, Inc.; San Diego CA, USA) that generated paired-end reads of 75 nucleotides