Illumina HiSeq 2500 sequencing; RNA sequencing of primary isolated unstimulated murine splenic cDC from specific pathogen free (SPF), Germ Free (GF) and Interferon alpha/beta receptor 1 (Ifnar) knock out mice.
RNA sequencing of primary isolated unstimulated murine splenic cDC from specific pathogen free (SPF), Germ Free (GF) and Interferon alpha/beta receptor 1 (Ifnar) knock out mice.
Cell were sorted and lysed in RLT Plus Buffer. RNA was isolated using the RNeasy plus micro kit. Barcoded mRNA seq libraries were prepared from 100 ng of total RNA (RIN>8) using a NEBnext poly(A) mRNA Magnetic Isolation Module and NEBnext UltraII lib prep kit for Illumina.
Experimental Factor: strain
germ free C57BL/6
Experimental Factor: genotype
wild type genotype