Whole genome sequencing of tea plant (Camellia assamica)
Whole genome sequencing (WGS) library was prepared with Illumina-compatible NEXTflex Rapid DNA sequencing kit (BIOO Scientific, Austin, Texas, U.S.A.) at Genotypic Technology Pvt. Ltd., Bangalore, India. Briefly, DNA was sheared using Covaris S2 sonicator(Covaris, Woburn, Massachusetts, USA) to generate approx. fragment size distribution from 200bp to 500bp. The fragment size distribution was checked on Agilent TapeStation and subsequently purified using High prepmagnetic beads (Magbio). Purified fragments were end-repaired, adenylated and ligated to Illumina multiplex barcode adaptors as per NEXTFlex RapidDNAsequencing kit protocol. Adapter-ligated DNA was purified using High prepbeads. Resultant fragments were amplified for 7cyclesof PCR using Illumina-compatible primers provided in the NEXTFlexRapid DNA sequencing kit.Final  PCR  product  (sequencing  library)  was  purified with High prepbeads,  followed  by  library quality  control  check. Illumina-compatible  sequencing library  wasquantified  by  Qubit  fluorometer (Thermo  Fisher  Scientific, MA,  USA)  and  its  fragment  size  distribution  was analyzed  on Agilent 2200 Tapestation.