GSM1602509: TG biological rep5; Mus musculus; RNA-Seq
Adult wildtype and transgenic mouse cardiomyocytes were isolated via the Langendorff perfusion method. Total cardiomyocyte RNA was then isolated using the RNeasy purification Kit (Qiagen) according to the manufacturer protocol. Library preparation for RNA-Seq was performed using the TruSeq RNA Sample Preparation Kit (Illumina, Cat. N°RS-122-2002) starting from 500 ng of total RNA according to Illumina's instructions. Accurate quantitation of cDNA libraries was performed by using the QuantiFluor™ dsDNA System (Promega). The size range of final cDNA libraries was determined applying the DNA 1000 chip on the Bioanalyzer 2100 from Agilent (280 bp). The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the HiSeq2000  following the manufacturer's protocols.
GEO Accession
GSM1602509