GSM4417173: EHF_NMPs_G6; Mus musculus; RNA-Seq
A region immediately lateral to the posterior edge of the node and lateral to the primitive streak, extending to about half the length of the primitive streak was microdissected in M2 medium using hand-pulled solid glass needles. Single-cells were sorted by Fluorescence-activated cell sorting with a FACS Aria II (BD Biosciences) into individual wells of a 96-well PCR plate containing the ERCC RNA spike-in Mix (Ambion,#4456740) diluted (1:100.000) Libraries from a total of 91 single cells were prepared using a Nextera XT index kit (Illumina)
GEO Accession
GSM4417173