GSM4274260: OVD_6hr_2_3; Drosophila melanogaster; RNA-Seq
Females were etherized (unmated, 6hrs, or 24hrs post-mating) and the female reproductive tract (FRT)  removed in a drop of 1x PBS. The FRT was then microdissected into 6 discrete tissues. Tissues were rinsed in PBS and transferred directly to Trizol. Pooled tissue collections were homogenized with a pellet pestle and RNA was isolated using a phenol-chloroform extraction with phase-lock gels (ThermoFisher) and an overnight precipitation in isoproponal and 3M sodium acetate with 1.5ul glycogen (LifeTechnologies) at -18 C to maximize RNA recovery. The precipitation was followed by DNAse I (LifeTechnologies) treatment for 30 min at 37 C and a second RNA isolation. RNA was re-suspended in RNase-free H2O RNA libraries were prepared for sequencing using standard protocols by Beijing Genomics Institute (BGI)
GEO Accession
GSM4274260