GSM1680991: Y&Z pooled lymph node B cell day 10 post boost; Mus musculus; RNA-Seq
RNA was extracted from frozen samples with the Qiagen RNeasy micro kit. Concentrations were determined by UV spectrophotometry (Nanodrop) and integrity of ribosomal RNA was confirmed with an Agilent Bioanalyzer. Polyadenylated RNA was extracted from total RNA, fragmented, ligated to sequencing and indexing adapters, and PCR amplified using Illumina TruSeq RNA kits as recommended by the kit instructions. Each sample represents the RNA collected from 6 mice. In some cases the RNA from Y and Z pools (3 mice per pool) was mixed prior to sequencing library construction. In other cases the Y and Z pools (3 mice per pool) had separate libraries and indexing adapters and the reads from two libraries were concatenated. The treatment and extraction protocols for Y and Z pools was identical; these pool names are arbitrary identifiers for the convenience of the laboratory.
GEO Accession
GSM1680991