GBStools HapMap data
Genomic DNA (50 ng) was digested with BpuEI, BsaXI, and CspCI. The digestion product was purified by spin column. DNA end repair, 3' monoadenylation, and ligation of sequencing adapters were performed as described in the Illumina TruSeq DNA Sample Preparation Guide. We used sequencing adapters derived from the TruSeq adapters, but with custom barcodes. The ligation products were amplified by 10 cycles of PCR. Inserts between 350-650 bp were size selected on a Caliper Labchip.