Viral assemblages associated with Pyura chilensis (Urochordata)
Viruses were prepared from Pyura chilensis gonads using the filter-chloroform-nuclease approach of Ng et al., 2011. Viral DNA was extracted using the Zymo Viral DNA kit. Nucleic acids were then amplified using the GenomePlex (for libraries labeled "A") or the Genomiphi kit (for libraries labeled "B").