Bacterial diversity of Halichondria panicea after ex situ cultivation
V3-V4 region of the  16S rRNA gene was amplified using the universal bacterial primer pair  S-D-Bact-0341-b-S-17 (5-CCTACGGGNGGCWGCAG-3) / S-D-Bact-0785-a-A-21  (5-GACTACHVGGGTATCTAATCC-3). Libraries were multiplexed using Nextera XT v2  indices and sequenced on a MiSeq System with v3 chemistry and 2 x 300