GSM3073231: Nectary line 412 replicate 1; Helianthus annuus; RNA-Seq
RNA was extracted from nectaries by mechanical disruption, with a microcentrifuge pestle, and using the RNAqueous®-Micro scale RNA isolation kit (Ambion, Austin, TX) with Plant RNA Isolation Aid (Ambion, Austin, TX). Agarose gel electrophoresis and UV spectrophotometry were used to assess RNA quality for all samples prior to submission to the University of Minnesota Genomics Center for barcoded library creation and Illumina HiSeq 2500 sequencing. Twenty-four TruSeq RNA v2 libraries were created (triplicate samples for nectaries from seven lines and triplicate leaf samples from one line) and sequenced via 50 bp, paired-end runs on the HiSeq 2500 using Rapid chemistry. All libraries were pooled and sequenced across two lanes to achieve the equivalent of approximately one lane of output. This generated 330 M reads and the average quality scores were above Q30. Illumina's Truseq RNA Preparation v2 kit (RS-122-2001)
GEO Accession
GSM3073231