RNAseq of Trypanosoma brucei brucei: Short stumpy parasites in RPMI media with proline and threonine
Total RNA was normalized, and converted to cDNA using the TruSeq Stranded Total RNA Library Prep Kit (Illumina).  Final sequencing products were validated for size on an Agilent Bioanalyzer 2100 (Agilent Technologies, Waldbronn, Germany)