RNA seq of T. neapolitana cells treated with CO2
Indexed libraries were prepared from 4 g/ea purified RNA with TruSeq Stranded mRNA Sample Prep Kit (Illumina) according to the manufacturers instructions. Libraries were quantified using the Agilent 2100 Bioanalyzer (Agilent Technologies) and pooled such that each index-tagged sample was present in equimolar amounts, with a final concentration of the pooled samples of 2 nM. The pooled samples were subject to cluster generation and sequencing using an Illumina HiSeq 2500 System (Illumina)in a 2 100 paired-end format at a final concentration of 8 pmol