MicroRNA identification in Viscum album
Small RNAs with a length of 18-30nt were gel purified from total RNA of the sample V. album. After being ligated to a 5' and 3' adaptors, the ligated products were used for cDNA synthesis, followed by acrylamide gel purification and PCR amplification to generate small RNA library. Small RNA reads with a length of 49nt were produced by Illumina and then used for data processing, miRNA identification, and target prediction.