GSM2651135: CAF_HiP culture rep1; Homo sapiens; RNA-Seq
Cells were trypsinized and pelleted for total RNA extraction. The AmpliSeq transcriptome libraries were prepared Ion AmpliSeq™ Transcriptome Human Gene Expression Kit. As described before (Li et al., 2015), A barcoded cDNA library is first generated with SuperScript® VILO™ cDNA Synthesis kit from 10 ng of total RNA. Then cDNA is amplified using Ion AmpliSeq™ technology to accurately maintain expression levels of all targeted genes. Amplified cDNA Libraries were evaluated for quality and quantified using Agilent Bioanalyzer High sensitivity chip. Libraries were then diluted to 100pM and pooled equally, with eight individual samples per pool. Pooled libraries were amplified using emulsion PCR on Ion Torrent OneTouch2 instruments (OT2) and enriched following manufactures instructions. Templated libraries were then sequenced on Ion Torrent Proton™ sequencing system, using Ion PI sequencing 200 kit v2.
GEO Accession
GSM2651135