RNA-seq of adult Pogonichthys macrolepidotus: Pooled mixed tissues
Tissues from several adult fish exposed to various stressors were homogenized using a TissueLyser (Qiagen) in Buffer RLT Plus (RNeasy Plus Mini Kit; Qiagen) and 350 ?l of the homogenate was used for the RNA extraction on a Qiacube (Qiagen) following manufacturers protocols and then pooled in equal amounts. RNA quality was assessed using a Bioanalyzer (Agilent). Total RNA was used to prepare a cDNA library using a KAPA Stranded mRNA-Seq Kit (KAPA Biosystems). Sequencing was conducted on one lane on an Illumina HiSeq 2000 to produce 100 base pair paired-end reads.