GSM3074502: WistarHan_oocyte_RNAseq_rep1; Rattus norvegicus; RNA-Seq
RNA-seq: total RNA was isolated from 100-200 mouse or rat oocytes using TriReagent, and ribosomal RNA was depleted using NEB Next rRNA Depletion kit according to manufacturer's instruction. Double stranded cDNA was synthesized using NEB Next first strand and second strand synthesis modules. Libraries were constructed using a custom protocol (Brind'Amour et al. 2015) and amplified for 10-12 PCR cycles (RNA) or 15 PCR cycles (ChIP). 75 bp or 100bp paired-end libraries were sequenced on the NextSeq 500 or HiSeq 2500 platform an according to manufacturer's protocols.
GEO Accession
GSM3074502