Prp16_5control
Prp16 was immunoprecipitated from yeast cells, which is expected to pull down spliceosomal complexes and lariats. Then, RNAs were extracted from these IP fractions and the treated with XRN1/CIP/RNase R to remove linear RNAs. This experiment serves as a control, so the rDbr1 treatment was ignored and the 5'end of RNAs were sequenced to reveal the background.