Genome sequencing of Pseudomonas sp. BF61 isolated  from an on-farm biopurification system
Standard NEXTERA protocol. Genomic DNA was quantified by Qubit (Thermo Fisher). 5-10 ng of genomic DNA was used in a 1:10 reaction of the Nextera DNA Flex Library Prep protocol . Tagmented DNA was amplified for 12 PCR cycles with 1 minute extension time.