MinION sequencing
Whole genome sequencing of the bacteriophage lambda genome. Read until has been applied to select reads whose start site maps to 15-25 kb of the genome. Read until is only applied to all channels.
Lambda whole genome libraries were prepared without PCR amplification according to the standard protocol using version 5 library preparation kits (ONT, SEQ-MAP006) supplied by Oxford Nanopore Technologies (ONT). This protocol has been extensively described by Ip et al. Library was sequenced on a Mk1 minION using FLO-MAP103 flow cells.