GSM4118098: PTBP1-SIM-2; Homo sapiens; OTHER
Samples were prepared according to the eCLIP protocol (Van Nostrand et al. 2016 Nat Methods.) For each sample, 2x10^7 cells were UV crosslinked at 400mJ/cm2 constant energy. Cells were lysed on ice for 15 min in 1 mL lysis buffer containing 50 mM Tris-HCl pH 7.4, 100 mM NaCl, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate, Halt protease inhibitor cocktail (ThermoFisher Scientific). Cells were passed through 27-gauge needles several times before sonication (Biorupter, Diagenode) for 3 min at low setting with 30 second on/30 second off duty cycle at 4°C. eCLIP and size-matched input libraries were constructed according to the eCLIP protocol (Van Nostrand et al. 2016 Nat Methods.)
GEO Accession
GSM4118098