NextSeq 500 paired end sequencing; RNAseq on PBMC of three patients with Familial Hemophagocytic Lymphohistiocytosis Type 5 (FHL5) and three age and sex-matched controls
RNAseq on PBMC of three patients with Familial Hemophagocytic Lymphohistiocytosis Type 5 (FHL5) and three age and sex-matched controls
Standard venopuncture followed by ficoll extraction of PBMC Total RNA was isolated from Peripheral Blood Mononuclear Cells (PBMC) using the RNeasy® Mini Kit (Qiagen, Hilden, Germany) Total RNA Sequencing libraries were prepared with SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (TaKaRa, Kusatsu, Japan), according to the manufacturer's protocol.
Experimental Factor: disease
normal