GSM3834100: SAT-SVF_2; Homo sapiens; RNA-Seq
Cells were lysed with TRIzol (Invitrogen) with the following total RNA isolation (Purelink RNA Micro Scale Kit, Invitrogen) and DNAse treatment (Sigma). For the 1 µg of isolated RNA, we performed rRNA depletion (RiboCop rRNA Depletion Kit V1.2, 037.96, Lexogen) followed by DNA library preparation (SENSE Total RNA-Seq Library Prep Kit, 042.96, Lexogen). DNA libraries were sequenced on HiSeq 2000 (Illumina) using 2 × 125 bp chemistry.
GEO Accession
GSM3834100