GSM4417291: Pm_mantle_a; Pecten maximus; RNA-Seq
Cells were disrupted manually with a pestle in liquid nitrogen before RNA extraction using RNeasy universal kit (Qiagen). Libraries were made at the Wellcome Sanger Institute. These were made according to their autoRNA library preparation process, with poly(A) pulldown, fragmentation, 1st and 2nd strand synthesis, end prep and ligation using the NEB Ultra II RNA custom kit (New England Biolabs, Massachusetts, United States) on an Agilent Bravo WS automation system (Agilent, California, United States). 14 rounds of PCR were used in library construction.
GEO Accession
GSM4417291