metagenome of enriched nanoflagellate Lenisia limosa and bacterial symbionts
DNA was extracted from a co-culture of Lenisia limosa with bacterial symbionts following the protocol of  Zhou et al. (1996, AEM 62:316-322) with minor modifications. For metagenome sequencing, 50 ng of extracted DNA were used to prepare an illumina Nextera library, following the Nextera DNA Sample Preparation Guide. The library was sequenced on a MiSeq (2x250bases paired end run, v2 chemistry).