GSM3833038: casein-1; Rattus norvegicus; RNA-Seq
Total RNA was extracted from cecal tissue by using Takara MiniBEST universal RNA extraction kit (Takara, Kusatsu, Japan). RNA degradation and contamination was monitored on 1% agarose gels. RNA purity was checked using the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA). RNA concentration was measured using Qubit® RNA Assay Kit in Qubit® 2.0 Flurometer (Life Technologies, CA, USA). RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM3833038