Salvelinus fontinalis cDNA
Liver tissue was collected from 22 fish, comprising eight individuals from a freshwater population (Rupert River, Québec - 48°44’N, 68°05’W), eight individuals from an anadromous population (Laval River Québec - 51°05’N, 73°41’W) (Audet and Bernatchez 2004 ; Sauvage et al. 2010), as well as six F1 individuals from these two populations (Sauvage et al., in prep). Total RNA was extracted using the Purelink Micro to Midi total RNA purification system according to the manufacturer’s recommendations. Evrogen (Moscow, Russia) performed a full-length cDNA amplification and normalization on 2µg of mRNA. The cDNA synthesis was performed using the SMART approach (Zhu et al. 2001). The amplified cDNA was then normalized using the DSN normalization method (Zhulidov et al. 2004). Normalization included cDNA denaturation/reassociation, treatment by duplex-specific nucleases (DSN, Shagin et al. 2002), and amplification of normalized fraction by PCR.   A total of 5µg of double stranded cDNA was sequenced on a GS-FLX Titanium sequencer using Roche's published protocol (Margulies et al. 2005) at the Genome Québec Innovation Center (McGill University, Montreal, Canada).