GSM2810883: pRyhB_2; Shewanella oneidensis; RNA-Seq
RNA was extracted using Trizol, combined with cleaning on a QIAGEN RNeasy column. Samples were DNase treated and re-purified on a RNeasy column. Quality of RNA was assessed using a Fragment Analyzer (Advanched Analytical Technologies) RNA sequencing libraries were constructed after rRNA depletion (with the Illumina Ribo-Zero rRNA removal kit) using the Truseq stranded mRNA library prep kit (without polyA selection using the strand-specific kit (Illumina, San Diego, CA, USA)). Library construction and RNA sequencing was performed at the Lausanne Genomic Technologies Facility at the University of Lausanne, Switzerland.
GEO Accession
GSM2810883