GSM2650721: HPBALL_RUNX1-On_MiSeq_rep1; Homo sapiens; RNA-Seq
RNA was isolated from live cells with TRIzol reagent followed by purification over PureLink RNA mini kit columns (Invitrogen). RNA-seq was performed using a Poly-A library construction protocol with strand specific cDNA synthesis and 8 cycles of PCR. Purification of polyA+ mRNA and mRNA(-) was done by Flow-through total RNA using MultiMACS 96 Separation Unit. For library construction, 96-well Plate-based Strand-specific cDNA Synthesis using Maxima H Minus Strand Specific 96-well was used for Illumina MiSeq Sequencing.
GEO Accession
GSM2650721