Illumina HiSeq 2500 paired end sequencing; RNA-Seq analysis of MCV-miR-M1 mimic-transfected HEK293 cells
RNA-Seq analysis of MCV-miR-M1 mimic-transfected HEK293 cells
293 cells were transfected with either MCV-miR-M1-5p, MCV-miR-M1-3p or control mimic (Thermo Fisher Scientific). RNA extraction was carried out using Arum total RNA extraction kit (Bio-Rad) and confirmation of MCV miRNA 5p and 3p expression via stem loop qRT-PCR. Total RNA libraries were prepared using TruSeq Stranded Total RNA Sample Prep Kit (Illumina, USA).
Experimental Factor: genotype
MCV-miR-M1-3p