GSM2214680: Type 1 skeletal muscle fibers; Homo sapiens; Bisulfite-Seq
DNA extraction from 17 to 44 single muscle fibers (3 ± 1 mm in length) was carried out using the QIAamp® DNA micro kit (Qiagen) Libraries were prepared from 32 ng of genomic DNA digested with 40 units of BfaI, 40 units of MseI, and 80 units of MspI and the fragments produced were ligated to pre-annealed adapters containing 5’-methylcytosine instead of cytosine. Adapter-ligated fragments were filled in and 3’ terminal-A extended, and then purified using the Zymo Research DNA Clean & Concentrator™– 5 kit (D4003). Size-selected DNA fragments of 40-350 bp were bisulfite treated using the EZ DNA Methylation– Lightning kit (Zymo Research, D5030). PCR was performed and the size and concentration of the fragments were confirmed on the Agilent 2200 TapeStation.
GEO Accession
GSM2214680