WXS of human cfDNA: Sample CRPC_468.ctDNA
Blood was fractionated via sequential centrifugation, and plasma containing cfDNA was extracted using the QIAsymphony DSP Circulating DNA Kit (Qiagen, kit #937556). End repair, A-tailing, ligation of Illumina-compatible adapters and PCR amplification was performed on 3-20ng cfDNA using the Kapa Hyper Prep Kit (Kapa, kit #KK8504) to create sequencing libraries. 500ng of the sequencing library was used as input into the hybrid selection portion of the Nextera Rapid Capture Kit (Illumina, kit #FC-140-1083) in order to pull down exonic regions of the genome for whole-exome sequencing.
alignment_software
BWA-0.5.9-tpx