GSM1246710: Lung Cancer Cell Line RVH6849; Homo sapiens; RNA-Seq
Total RNA was isolated from confluent cell lines or fresh-frozen xenograft tissues using Trizol reagent (Life Technologies, Carlsbad, CA, USA) according to the manufacturer's instructions, followed by Dnase I treatment (Life Technologies) and purification using the Qiagen RNeasey kit (Qiagen, Venlo, Netherlands).  RNA quantity and quality was assessed using the Qubit Fluorometer (Life Technologies), NanoDrop 1000 (Nanodrop Technologies, Wilmington, DE, USA), and Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA).  Five micrograms of total RNA was fractionated using the flashPAGETM fractionator system (Life Technologies) and small RNAs (~<40nt) were recovered by ethanol precipitation.  Small RNA enrichment was confirmed using the small RNA Lab-on-a-Chip kit and the Bioanalyzer 2100 (Agilent). Libraries were prepared from fractionated small RNA samples as per Illumina TruSeq Small RNA protocol (version D) (Illumina, San Diego, CA, USA).
GEO Accession
GSM1246710