Bisulfite-Seq of Zootermopsis nevadensis: alate female: Sample Znev_dAF1
"Genomic DNA was isolated from a pool of five individual termites (except for male alates where only four individuals were used) using MasterPure(TM) Complete DNA and RNA Purification Kit (Epicentre) according to manufacturer's protocol. The extracted DNA was suspended in 35uL TE buffer.  DNA concentration was determined by Qubit(R) 2.0 fluorometer (Life Technologies). Bisulfite converted DNA and mRNA library preparation was performed in Georgia (http://dna.uga.edu), and sequencing was performed using the Illumina NextSeq500 platform."