DNAseq sequencing of Lupinus albus leaves
Genomic DNA was extracted from fresh leaves with the NucleoSpin Plant II kit (Macherey-Nagel), using a chaotropic salt based silica membrane purification method,  following the manufacturers procedure. DNA concentration was determined with a NanoDrop1000 and a Qubit Fluorometer (Kit Quant-iTTM, dsDNA BR Assay) and DNA quality/integrity was checked via Agarose Gel Electrophoresis. DNA amount required was 5 to 20 microgrammes per sample. Qualified genomic DNA of each sample was fragmented randomly. After electrophoresis, DNA fragments of desired length (>800pb) were gel purified. Pair-end sequencing was performed from a DNA short insert size library (~ 500bp). Adapter ligation and DNA cluster preparation were performed and subjected to sequencing using Illumina HiSeq2000 yielding 100bp PE reads (BGI Hong-Kong). To ensure the data quality, the adapters were first deleted in the reads from the raw sequencing data, then the reads which contain more than 50% low quality bases (quality value  5) were removed. The Q20 bases rate of the data production (clean reads) was above 94.32 %.