ddRADseq of Montipora capitata: coral tissue
DNA extractions were performed on ethanol-preserved coral tissues that were dried and pulverized. Extracted DNA was sent to the University of Minnesota Genomics Center for Genotyping-by-Sequencing service. ddRAD was the chosen method and 96 dual-indexed libraries were created. Restriction enzymes BtgI and TaqI were used and fragments were size selected to 300-744bp. All libraries were pooled and sequenced using half a lane of NextSeq 500 in 1x151bp, single-end configuration.