Hir_TrueMate_5_6
Ion Torrent mate-pair sequencing - mate-pair library with 3-6Kb fragment was prepared from a sample genomic DNA with reagent kits Ion TrueMate Library Reagents (Life Technologies). To conduct emulsion PCR, the Ion PGM  template OT2 400 kit (Life Technologies) was utilised. Sequencing was performed in accordance with manufacturer protocol for the genome analyser Ion Torrent PGM (Life Technologies) using an Ion 318 chip and Ion PGM  sequencing 400 kit v2 (Life Technologies). For separation non-mate reads and spliting true mate reads we use script  matePairingSplitReads.py