Sup Fig 1c:  Observed Percentage of Low-Input Target Sequencing Yield vs. Expected Percentage by Molarity: 2kb libraries
A serial dilution of one known 2kb amplicon into a second known 2kb amplicon was performed, beginning with a 10% spike-in by molarity and then serial diluting six times down to a spike-in ratio of 0.15625%.  Libraries were then prepared from each serial dilution using 500 nanograms as input for each and were subsequently sequenced.