Sequel sequencing; Single-cell RNA counting at allele- and isoform-resolution using Smart-seq3
Single-cell RNA counting at allele- and isoform-resolution using Smart-seq3
Single-cells were FACS sorted into 384-well plates No extraction was performed. Libraries were constructed using Smart-seq3. Full protocol details are found here: https://www.protocols.io/view/smart-seq3-protocol-7dnhi5e Full-length cDNA generated using Smart-seq3 was further amplified to yield ~1 µg of template for the SMRTbellTM Template Prep Kit 1.0-SPv3. PacBio library preparation was performed by NGI Uppsala.
Experimental Factor: single cell identifier
J5 GACTGTGTGTTCTGGA