Ammonia unamended soil from 56-days aerobic dynamic: day 14
This paired-end SRA file contains the V1-V3 16S rRNA raw sequences as a multiplexed library from the 18 ammonia unamended soil samples for Day 14 of the 56-days aerobic dynamic. The paired-end fastq files must be fed into the split_libraries_fastq.py script to demultiplex each Sample by its 16-bp unique barcode. The mapping file to be used is the following; where '$' must be changed to tabulation ('\t') and '@' to a new line ('\n'): SampleID$BarcodeSequence$LinkerPrimerSequence$ReversePrimer$soil_use$dinamic$Practice$Fertilizer$Day$Treatment$Pract.Day$Pract.Fertil.Day$Day.Fertilizer$Soil$OH$Description@DNA235$TAAGGCGATAGATCGC$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$unamended$CT$0$14$CT0$CT.14$CT.0.14$D14.0$111$1$Soil.CTRL.91@DNA236$TAAGGCGACTCTCTAT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$unamended$CT$0$14$CT0$CT.14$CT.0.14$D14.0$211$2$Soil.CTRL.92@DNA237$TAAGGCGATATCCTCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$unamended$CT$0$14$CT0$CT.14$CT.0.14$D14.0$311$3$Soil.CTRL.93@DNA238$TAAGGCGAAGAGTAGA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$unamended$CT$300$14$CT300$CT.14$CT.300.14$D14.300$115$4$Soil.CTRL.94@DNA239$TAAGGCGAGTAAGGAG$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$unamended$CT$300$14$CT300$CT.14$CT.300.14$D14.300$215$5$Soil.CTRL.95@DNA240$TAAGGCGAACTGCATA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$unamended$CT$300$14$CT300$CT.14$CT.300.14$D14.300$315$6$Soil.CTRL.96@DNA241$TAAGGCGAAAGGAGTA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBB$0$14$PBB0$PBB.14$PBB.0.14$D14.0$121$7$Soil.CTRL.97@DNA242$TAAGGCGACTAAGCCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBB$0$14$PBB0$PBB.14$PBB.0.14$D14.0$221$8$Soil.CTRL.98@DNA243$CGTACTAGTAGATCGC$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBB$0$14$PBB0$PBB.14$PBB.0.14$D14.0$321$9$Soil.CTRL.99@DNA244$CGTACTAGCTCTCTAT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBB$300$14$PBB300$PBB.14$PBB.300.14$D14.300$125$10$Soil.CTRL.100@DNA245$CGTACTAGTATCCTCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBB$300$14$PBB300$PBB.14$PBB.300.14$D14.300$225$11$Soil.CTRL.101@DNA246$CGTACTAGAGAGTAGA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBB$300$14$PBB300$PBB.14$PBB.300.14$D14.300$325$12$Soil.CTRL.102@DNA247$CGTACTAGGTAAGGAG$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBC$0$14$PBC0$PBC.14$PBC.0.14$D14.0$151$13$Soil.CTRL.103@DNA248$CGTACTAGACTGCATA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBC$0$14$PBC0$PBC.14$PBC.0.14$D14.0$251$14$Soil.CTRL.104@DNA249$CGTACTAGAAGGAGTA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBC$0$14$PBC0$PBC.14$PBC.0.14$D14.0$351$15$Soil.CTRL.105@DNA250$CGTACTAGCTAAGCCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBC$300$14$PBC300$PBC.14$PBC.300.14$D14.300$155$16$Soil.CTRL.106@DNA251$AGGCAGAATAGATCGC$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBC$300$14$PBC300$PBC.14$PBC.300.14$D14.300$255$17$Soil.CTRL.107@DNA252$AGGCAGAACTCTCTAT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBC$300$14$PBC300$PBC.14$PBC.300.14$D14.300$355$18$Soil.CTRL.108