GSM1922448: obe1/obe2_rep3; Drosophila melanogaster; RNA-Seq
Embryos were ground in microfuge tubes in 50 ul Trizol reagent using plastic pestles. An additional 450 ul Trizol was then added and the samples were incubated for 5 min at RT. Next, 100 ul chloroform was added, and the samples were incubated for 3 min at RT. The samples were centrifuged at 12,000 g for 15 min to separate the aqueous and organic phases. At this point the standard Trizol protocols for total RNA extraction were followed. The standard PolyA TruSeq RNA library preparation protocol