GSM2218809: AC_AGO_IP_C2; Xenopus laevis; OTHER
For small RNAs and total RNAs: Tissue samples were lysed in Trizol, and RNA was extracted and treated with DNaseI using the Direct-zol RNA mini-prep kit (Zymo). For AGO IP RNA: Ago-RNP immunoprecipitations were carried out as a modification of Keene et al. (2006). RNA samples were processed for library preparation using the NEBNext Multiplex Small RNA Library Prep Set for Illumina kit according to manufacturer's instructions. Sequencing libraries were prepared from the immunoprecipitated RNA samples using the ScriptSeqTM Complete Gold Kit - Low Input (Epicentre Technologies), with rRNA depleted protocol.
GEO Accession
GSM2218809