GSM3141420: KPC_Tumor_CAFs biological rep1; Mus musculus; RNA-Seq
KPC tumors were dissociated into single cell suspensions at 37C, 600rpm for 45 min using collagenese, liberase and Dnase,  and passed through a 100 um FlowMi cell strainer.  Cells were stained with DAPI, anti-mouse CD31 Alexa Fluor 647, anti-mouse CD45 Alexa FLuor 647, anti-mouse PDPN Alexa APC/Cy7, anti-mouse PDGFRa PE. DAPI- cells and DAPI-CD45-CD31-EpCAM-PDPN+ cells were sorted in 2 different collection tubes. Viability was confirmed to be >75% using a Countess FL II cell counter (Life Technologies #AMQAF1000) before proceeding to single cell library prep. Single cell libraries were prepared using a 10X Genomics Chromium Controller (cat #120223) and the 10X Genomics Single Cell 3' Gene Expression kit (cat #120237) according to the manufacturer's instructions Single cell 3' digital gene expression with cell barcodes and unique molecular identifiers (UMIs).
GEO Accession
GSM3141420