IntronD_and_IntronP
HindIII digestion to remove wtTRs followed by gel purification. mixed IntronD and IntronP vectors. DNA from purified rAAV viral vector preparations were treated with PreCR® Repair Mix (NEB, # M0309L) for nicked and end repair before directly ligating to SMRT-Bell adapters at a 1:1, adapter to vector molecular ratio and sequenced on a Pacific Biosciences RSII Instrument