RNA-Seq of saccharina japonica: 1/3 blade in Apr
mRNA was enriched with Oligo (dT) beads, cleaved into small pieces with fragmentation buffer, and then reverse transcribed into cDNA with random primers. Second-strand cDNA was subsequently generated using RNase H and DNA polymerase I. The cDNA fragments were purified with QiaQuick PCR extraction kit (Qiagen, Germany), end repaired, poly(A) added, and ligated to adaptors. After PCR amplification, the cDNA libraries were paired-end sequenced on Illumina HiSeqTM 2500 platform (San Diego, CA, USA) by Gene Denovo Biotechnology Co. (Guangzhou, China).