GSM3379573: NAc_Cocaine_2_INO80_ChIP_1; Rattus norvegicus; ChIP-Seq
Brain tissues were crosslinked and quenched. Samples were washed thoroughly before being subjected to lysis and sonication. Samples were then incubated with INO80 antibodies (7.5 μg/sample) bound to M-280 Dynabeads on a rotator at 4°C overnight. The following day, immunoprecipitates were washed, eluted and reverse crosslinked. Following RNA and protein digestion, DNA fragments were purified using a Qiagen PCR purification kit. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM3379573