GSM2124750: control-28; Homo sapiens; RNA-Seq
Frozen postmortem dorsal striatum samples were transferred to RNAlater-ICE (Ambion) and stored at -20C upon receipt. Total RNA was extracted from dissected caudate-putamen tissue by Trizol (Life Technologies), column-purified by the RNeasy Lipid Tissue Kit (Qiagen) and treated with the Turbo DNA-free Kit (Life Technologies). RNA samples were assayed on the BioAnalyzer (Agilent, Santa Clara, CA) to obtain RNA integrity number (RIN) values. Ribosomal RNA (rRNA) was depleted from samples using the RiboMinus Eukaryote Kit v2 (Life Technologies). Strand-specific cDNA libraries were made using the Next Ultra Directional RNA Library Prep Kit (NEB) and sequenced on the Illumina HiSeq2000.
GEO Accession
GSM2124750