The transcriptome sequencing of seeds in Brassica napus
B. napus cv Ninyou 12 was used as the source material. The leaves (4–5 euphylla period) and seeds at 25 days after pollination were harvested in the field and immediately frozen in liquid nitrogen and stored at –70°C for RNA extraction.The mRNA-seq library was constructed using Illumina’s TruSeq RNA Sample Preparation Kit (Illumina lnc, San Diego, CA, USA), and the isolation of mRNA, fragment interruption and RNA-Seq were performed by the company according to their standard protocol. Finally, the mRNA-seq library was constructed for sequencing using the Illumina HiSeqTM 2000 sequencing platform.