GSM4610383: C.elegans L4_BaP 0_rep1; Caenorhabditis elegans; RNA-Seq
The RNA isolation followed the standard procedures using Trizol reagent (Invitrogen), however modified to include a homogenization step with 0.5 mm glass beads (Sigma) to maximize cell breakage. Libraries were prepared according to Illumina's instructions. Briefly, mRNAs were purified using Poly(A) selection from total RNA and then fragmented. First strand of cDNA was synthesized using random priming, followed by the synthesis of the second strand of cDNA. The resulting double-strand cDNA was end repaired, phosphorylated and A-tailed. Adapter ligation and PCR amplification were performed, rendering the library ready for Illumina flow cell clustering and sequencing on an Illumina HiSeq 2500 following the manufacturer's protocols.
GEO Accession
GSM4610383