DNAseq Mycoplasma agalactiae 7784
M. agalactiae was grown 48 hours in 25 mL of Hayflick medium supplemented with sodium pyruvate at 37C as previously described. After, the genomic DNA using the MasterPure Complete DNA & RNA Purification Kit (MC85200, Lucigen) following the protocol described by the manufacturer. The genomic DNA was sheared to 100 bp fragments using a Covaris S2 device. Then, paired-end Illumina libraries were created following previously described protocols (Bentley et al. 2008) and the size selected was 125 bp. The resulting libraries were quantified on an Agilent Bioanalyzer chip (Agilent Technologies). Double-stranded templates were cluster amplified and sequenced on an Illumina GAII. Raw reads were analyzed by using the FastQC  tool (website: http://www.bioinformatics.bbsrc.ac.uk/projects/fastqc) for assessing the quality and the presence of adapters.