DNA-seq of Paracoccus kondratievae BJQ0001
Genomic DNA was treated into fragments with proper size (> 10 Kb) by g-TUBE (Covaris, USA) at first. Then the fragments are damage repaired and ends repaired. Both sides of DNA fragments are connected with hairpin adapter respectively to get a dumbbell structure, which is known as single-molecular real-time (SMRT) bell. After annealing, the SMRT bell is fixed at the bottom of the ZWM polymerase, and finally used for sequencing. The SMRT sequencing was performed using a PacBio RSII sequencer.