GSM4417324: sre1∆_pH8, 5; Cryptococcus neoformans; RNA-Seq
All cells were pelleted, flash frozen on dry ice, and lyophilized overnight. RNA was isolated using the Qiagen RNeasy Plant Minikit with  optional  on  column DNase  digestion  (Qiagen, Valencia, CA). RNA concentration and quality were measured using the Agilent 2100 Bioanalyzer. The NEBNext Poly(A) mRNA Magnetic Isolation Module was used to enrich for mRNA and the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina was used to prepare libraries (New England Biolabs, Ipswich, MA). Libraries were sequenced on the Illumina NextSeq 500 with 75 base pair, single-end reads.
GEO Accession
GSM4417324