Sequencing of 2 invertebrate kick samples, to test effects of sample sorting by biomass in DNA metabarcoding freshwater assessments
Two sample sites from the Schmalenau river system were sequenced on the HiSeq system. Macroinvertebrate kick samples were identified based on morphology and then sorted into 3 size fractions according to specimen size. Each fraction was extracted and sequences individually, and tissue for extraction pooled 1) adjusted by number of samples in each fraction and 2) pooled by tissue weight of each fraction, simulating that the sample has not been split in the first place. Please see publication for further details.