GSM3380682: B16-F10-flv-2; Mus musculus; RNA-Seq
Total RNA was extracted from the cells using Tri reagent (Sigma). RNase-free DNase was used for removing all genomic DNA contamination. The RNA was precipitated by Isopropanol (Sigma), washed by ice cold 75% ethanol (Sigma), and air dried prior to resuspension in 20 µl of DEPC-treated water. Purity and concentration of RNA was measured by NanoDrops (Thermo Scientific). The 260/280 ratio was 1.90-2.00 and the 260/230 ratio was 2.00-2.20 for all RNA Samples. Stranded polyA RNAseq was carried out using illumina dUTP protocol, 50bp single end
GEO Accession
GSM3380682