GSM1251901: Event1_Ear_Rep2; Zea mays; RNA-Seq
Total RNA was isolated from three biological replicates for each tissue using TRIzol (Invitrogen) according to the manufacturer's protocol. RNA quantity was determined with a Nanodrop 8000 spectrophotometer and integrity assessed by Bioanalyzer assay with RIN greater than 6. Two µg of total RNA was used for sequencing library preparation with Illumina TruSeq RNA Sample Prep Kit V2 following manufacturer’s protocol.  qPCR (SYBR PCR Master Mix, Applied Biosystem) was utilized to quantify sequencing libraries. Sequencing was performed with Illumina HiSeq2000 sequencing using 50 base pair reads.
GEO Accession
GSM1251901