GSM2991323: Stage 1 rep5 [ncRNA]; Bos taurus; ncRNA-Seq
RNA was isolated from a total of 20 EVs samples (4-5 replicates, 4 stages) using miRNeasy Mini kit (QIAGEN) according to the manufacturer's instructions. RNA quality and concentration were evaluated by Agilent 2100 Bioanalyzer Nano and small RNA assay (Agilent Technologies, Santa Clara, CA.) and NanoDrop (ThermoFisher). A total of 444 ng RNA was used for the preparation of each small RNA library using NEXTflex™ Small RNA-Seq Kit v3 (Bioo Scientific). Library preparation followed the manufacturer's instructions.
GEO Accession
GSM2991323