microRNA raw data of Megalobrama amblycephala involved in intermuscular bone development
Total RNA was isolated from 8 samples of 4 stages (each stage possessed two biological replicates) using RNAiso Plus Reagent (TaKaRa, China) according to the manufacturer’s protocol. RNA quality and quantity was measured using the NanoDrop 2000 (Thermo Scientific, USA) and Agilent 2100 Bioanalyzer (Agilent, USA). All the samples were standardized to 500 ng/μl. Afterwards, two samples of each stage were　mixed into one pool for microRNA sequencing. During the QC steps, Agilent 2100 Bioanaylzer and ABI StepOnePlus Real-Time PCR System were used in quantification and qualification of the sample libraries. Finally, the librariy were sequenced using an Illumina HiSeq™ 2000 platform (BGI, Shenzhen, China).