Tissue-wide RNA-seq expression profiles of Drosophila melanogaster
Tissue-wide RNA-seq expression profiles of Drosophila melanogaster
Wild-type Drosophila melanogaster (Canton S strain) were raised on standard medium on a 12:12 h L:D cycle, at 23C, and at 55% r.h. To facilitate the collection of accurately staged adults, a laying population of around 12 males and 12 females were transferred to fresh vials daily. When adults subsequently emerged, they were transferred to fresh vials on the day of emergence, and used 7 days later. Organism parts used: tubule (The full length of the Malpighian tubules including upper and lower ureter, severed at their insertion into the hindgut), testes (testes until they end up in the collecting duct), head (The complete head, severed at the neck). RNA extraction: QIAGEN RNeasy Mini columns. 30 wholeflies are pooled for RNA extraction, 100 testes are pooled for RNA extraction, 600pairs of tubules pooled for RNA extraction, 300 heads are pooled fro RNA extraction
Experimental Factor: ORGANISMPART
head