GSM3141511: SPR_LP_Rep1; Mus musculus; MBD-Seq
Genomic DNA was isolated from paternal sperm and offspring's mammary tissue (n=3/group). Methylated DNA was eluted by the MethylMiner Methylated DNA Enrichment Kit (Invitrogen).  Briefly, one microgram of genomic DNA was sheared by sonication and captured by MBD proteins. The methylated DNA was eluted and used to generate Methyl-CpG binding domain-based capture (MDBCap) libraries RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM3141511