GSM4611905: STAG2-KO Neural Tube rep1; Mus musculus; RNA-Seq
Whole mount KO and WT embryos at E9.5 (21-23 pairs of somites) were placed in cold PBS. The whole heart along with surrounding SHF regions and a section of heart-proximal neural tube were dissected, snap-frozen and stored at -80 ºC. Per genotype and region, 3 replicates from 3 embryos each were extracted using TRI reagent (Sigma T9424) and homogenized with syringe and needle (25-30G) Libraries were prepared using the QuantSeq 3' mRNA-seq Library Pr­ep Kit FWD (Lexogen)
GEO Accession
GSM4611905