GSM1681911: CD59 screen; Homo sapiens; OTHER
Genomic DNA was extracted from HAP1 cells using a QIAamp DNA Mini Kit (Qiagen). Linear PCR was performed using 2 mg of genomic DNA as template and the following primer: 5¢-Biotin-GGTCTCCAAATCTCGGTGGAAC-3¢. After 125 cycles of amplification, the linear PCR products were purified using Dynabeads (M280 Biotin Binder, Life Technologies). On-bead ligation of a 5¢ phosphorylated, 3¢ ddC linker was performed using Circligase II (Epicentre). The product was purified and used as a template for PCR to add Illumina adapter sequences.
GEO Accession
GSM1681911