GSM4263865: AL4; Mus musculus; RNA-Seq
Total RNA was isolated with TRIzol. RNA degradation and contamination were assayed on 1% agarose gels, and RNA purity was verified using a NanoPhotometer spectrophotometer. Concentration were measured using a Qubit RNA Assay Kit and a Qubit 2.0 Fluorometer. RNA integrity was assessed using an RNA Nano 6000 Assay Kit and a Bioanalyzer 2100 system. 3 μg RNA per qualified sample was used as input material for RNA library preparation. Sequencing libraries were generated using a NEBNext Ultra RNA Library Prep Kit for Illumina, and unique index codes were added to each sample. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM4263865