Cellulose methanization experiment in thermophilic conditions, archaeal diversity analyzed by 16S rRNA gene tag pyrosequencing
Archaeal tag-encoded FLX amplicon pyrosequencing (bTEFAP). The library was prepared by Research and Testing Laboratory (Lubbock, TX) based upon RTL protocols (www.researchandtesting.com). The PCR was performed with primers 28F and 519R. Tag-encoded FLX amplicon pyrosequencing analyses utilized Roche 454 FLX instrument with Titanium reagents, titanium procedures performed at the Research and Testing Laboratory (Lubbock, TX) based upon RTL protocols (www.researchandtesting.com).