GSM3535593: Tri-DS3, +OLIG2 shRNA,+Shh+Pur,D14; Homo sapiens; RNA-Seq
Total RNA was prepared with RNAeasy kit (Qiagen) (Chen et al., 2014) and libraries were constructed using 600 ng of total RNA from each sample and the TruSeqV2 kit from Illumina (Illumina, San Diego, CA) following manufacturers suggested protocol. The libraries were subjected to 75 bp paired read sequencing using a NextSeq500 Illumina sequencer to generate approximately 30 to 36 million paired-reads per sample.
GEO Accession
GSM3535593