Illumina HiSeq 2000 sequencing; Ribosome profiling and RNA-seq of in vitro differentiated human CD4+ Th1 cells.
Ribosome profiling and RNA-seq of in vitro differentiated human CD4+ Th1 cells.
Naive CD4+ T cells purified from the blood of healthy donors were polarized in vitro to TH1 in RPMI media with 10% FBS, 0.1% Penicillin/Streptomycin 0.1 % nonessential amino acids and  0,1% Sodium Pyruvate  at 37°C and 5% CO2. For RNAseq samples, RNA was extracted using the mirVana isolation kit according to manufacturer's instructions (mirVana ThermoFisher #AM 1560) Libraries for RNAseq were prepared using Illumina TruSeq Stranded Total RNA Library Prep Kit
Experimental Factor: protocol
RNA-seq