RNA-Seq of Escherichia coli plasmid pNDM-HK : small non-coding RNAs
Bacteria carrying pNDM-HK were grown in LB at 37 C under shaking conditions. Bacteria were harvested during early stationary phase. Total RNA was extracted using Trizol chloroform method while small RNA fractions were enriched by mirVana miRNA isolation kit. rRNA was removed by MICROBExpress Bacterial mRNA Enrichment kit and Ribo-Zero rRNA Removal Kit (Bacteria) following the manufacturers' protocol.