GSM4548083: E17Merkel_Pou4f3KO_ATACseq_rep2; Mus musculus; ATAC-seq
Merkel cells (Atoh1-GFP+) were purified from the skin of E17 Pou4f3 mutant animals by FACS sorting. Nuclei were extracted and chromatin were digested by tn5 transposase at 37°C for 20 minutes.DNA fragments were extracted using MiniElute columns (Qiagen), and then amplified by PCR. Library were amplified by PCR with indexed primers
GEO Accession
GSM4548083