Sequencing of Triticum aestivum isolated chromosome arm 7DS - 100bp readlength
Seeds of double ditelosomic line 7D of Triticum eastivum cv. Chinese Spring were germinated and root tips of young seedlings were used for the preparation of liquid suspensions of intact chromosomes. Chromosome arm 7DS was flow-sorted as telocentric chromosome in four batches of 39,000 chromosomes representing 30 ng DNA. The average purity in sorted fractions was 84%. Chromosomal DNA was purified and subsequently amplified using Illustra GenomiPhi V2. A total of 200 ng of pooled, amplified DNA was used to prepare an Illumina paired-end library which was sequenced on the Illumina GAIIx platform using standard protocols.