GSM3380586: DA#7; Mus musculus; Bisulfite-Seq
The genomic DNA is extracted from 1 million mouse stem cells (Two biological replicates each for wild type and Tet1 D2018A mutant) by using the DNeasy kit from Qiagen following the manufacturer's protocol. Then Methyl-Seq libraries were prepared using Accel-NGS® Methyl-Seq DNA Library Kit with 30 ng gDNA for each sample. Briefly, the single-stranded DNA fragments were end repaired, tailed of 3' ends and ligated with adapter. After adapter ligation DNA was PCR amplified with Illumina Truseq primers for 8 cycles. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on Illumina HiSeq 4000 with 150 bases pair-ended reads.
GEO Accession
GSM3380586