GSM1923355: BioRep1_mitoRP_9; Saccharomyces cerevisiae; RNA-Seq
sample processing: RNaseI treatment, anti-FLAG immunopurification, FLAG elution, RNA isolation by phenol/chloroform extraction, gel purification of 36-42 nt fragments Gel-purified RNA was treated with PNK and ligated to a pre-adenylated adapter using T4 RNA ligase 2, K227Q, truncated. Superscript III was used for reverse transcription and circ ligase for circularization. Illumina adapters were introduced by 10-14 cycles of PCR.
GEO Accession
GSM1923355