GSM1923244: hrde1 +sensor; Caenorhabditis elegans; ncRNA-Seq
Total RNA extracted using Trisure (BioLine) or Trizol (Invitrogen) according to manufacturer's instructions and followed by an overnight precipitation at -20C using isopropanol and glycogen as a carrier. Before library preparation, 1-5ug of total RNA was treated with 1ul of RNA polyphosphatase (Epicentre) for 30 minutes at 37C.  RNA was then extracted using Phenol/chloroform and precipitated overnight at -20C using Ethanol and Sodium Acetate.  Following this we followed the Illumina Truseq small RNA protocol according to the manufacturer's instructions
GEO Accession
GSM1923244