GSM1602676: siLuc_rep6; Mus musculus; RNA-Seq
Tumor RNA was isolated.  RNA quality was measured using an Agilent Bioanalyzer and RNA was quantified using a Qubit Fluorometer (Life Technologies) before sequencing library generation. RNAseq libraries were prepared using the ScriptSeq Complete Gold Kit (Epicentre). RNAseq libraries were uniquely indexed with custom primers (SriptSeq) and run on MiSeq (Illumina) to confirm quality of the library before sequencing to deeper coverage using a HiSeq2500 (Illumina). Approximately 50 million reads per sample were obtained.
GEO Accession
GSM1602676