Transcriptome analysis of the rice osmads13 mutant
Total RNA was extracted from pools of young inflorescences using the Pure Link RNA MiniKit (AMBION). Independent biological replicates were collected for the two genotypes (wild-type and osmads13 mutant). The quality and quantity of RNA samples were assessed by gel electrophoresis, BioAnalyzer and Nanodrop quantification. Upon treatment with TURBO DNAse I (AMBION), 4 g of each sample were used to produce sequencing libraries with the TruSeq mRNA sample preparation kit (Illumina).