GSM2054442: WT_Flag_CLIP; Yersinia pestis; RIP-Seq
For CLIP-seq, the cultures grown under the same conditions were collected and resuspended in 10 mM Tris-HCl (pH8.0), respectively. The pellets were dispersed on a petri dish and irritated uncovered with 400 mJ/cm2 of UV 254 nm to form the crosslinked RNA-protein complex. Bacterial cells were collected and lysed in RIP Lysis buffer and subjected to co-immunoprecipitation (Co-IP). RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM2054442