GSM2650173: Ox-48h-1; Mus musculus; miRNA-Seq
The total RNA was harvested using Trizol reagent. The integrity of total RNA was determined by the Agilent 2100 RNA Nano 6000 Assay Kit (Agilent Technologies, CA, USA) with suitable RNA samples having an RNA integrity number > 6.5. Approximately, 17 μg of small RNA-enriched total RNA was prepared for high-throughput sequencing. For each library, small RNA ranging from 14 to 36 nt was purified by 15% Tris-bora-EDTA polyacrylamide gel electrophoresis, and 3´ and 5´ adaptors were ligated with unique small RNA fractions. The modified small RNA was then reverse-transcribed and amplified by reverse transcription-PCR. Finally, the enriched cDNA was sequenced on a Illumina HiSeq 2500 according to the manufacturer’s instructions.
GEO Accession
GSM2650173