GSM3834405: RNA-seq_DOHH_g2; Homo sapiens; RNA-Seq
Cells were harvested in lysis buffer and RNA was extracted using the Direct-zol RNA miniprep kit (Zymoresearch) as described in McGlincy and Ingolia, Methods, 2017. RNA-seq libraries were prepared using the TruSeq Stranded Total RNA Library Prep Kit (Illumina) and sequenced using the 75 bp single-read protocol on a NextSeq 500 platform (Illumina).