Transcriptome of Gastric cancer cell line ACP03
The Charge Switch Total RNA Cell kit (Life Technologies, EUA) was used to isolate total RNA from ACP03 cells according to the manufacturer's protocol. The quality and quantity of the RNA were evaluated through on agarose gel and with Qbit system (Life Technologies, USA), respectively. Ribosomal RNAs were removed using RiboMinus ™ transcriptome Isolation Kit (Human / Mouse) from Life Technologies. At depleted RNA total was added RNA ERCC92 spike in (Life Technologies) internal calibrator in the ratio of 1 μl of the diluted 1/167 spike in RNA per 300 ng from depleted RNA total.