Ammonia amended soil from 56-days aerobic dynamic: day 5
This paired-end SRA file contains the V1-V3 16S rRNA raw sequences as a multiplexed library from the 18 ammonia amended soil samples for Day 5 of the 56-days aerobic dynamic. The paired-end fastq files must be fed into the split_libraries_fastq.py script to demultiplex each Sample by its 16-bp unique barcode. The mapping file to be used is the following; where '$' must be changed to tabulation ('\t') and '@' to a new line ('\n'): SampleID$BarcodeSequence$LinkerPrimerSequence$ReversePrimer$soil_use$dinamic$Practice$Fertilizer$Day$Treatment$Pract.Day$Pract.Fertil.Day$Day.Fertilizer$Soil$OH$Description@DNA55$TAAGGCGATAGATCGC$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$amended$CT$0$5$CT0$CT.5$CT.0.5$D5.0$111$1$Soil.NH4.55@DNA56$TAAGGCGACTCTCTAT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$amended$CT$0$5$CT0$CT.5$CT.0.5$D5.0$211$2$Soil.NH4.56@DNA57$TAAGGCGATATCCTCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$amended$CT$0$5$CT0$CT.5$CT.0.5$D5.0$311$3$Soil.NH4.57@DNA58$TAAGGCGAAGAGTAGA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$amended$CT$300$5$CT300$CT.5$CT.300.5$D5.300$115$4$Soil.NH4.58@DNA59$TAAGGCGAGTAAGGAG$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$amended$CT$300$5$CT300$CT.5$CT.300.5$D5.300$215$5$Soil.NH4.59@DNA60$TAAGGCGAACTGCATA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$amended$CT$300$5$CT300$CT.5$CT.300.5$D5.300$315$6$Soil.NH4.60@DNA61$TAAGGCGAAAGGAGTA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBB$0$5$PBB0$PBB.5$PBB.0.5$D5.0$121$7$Soil.NH4.61@DNA62$TAAGGCGACTAAGCCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBB$0$5$PBB0$PBB.5$PBB.0.5$D5.0$221$8$Soil.NH4.62@DNA63$CGTACTAGTAGATCGC$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBB$0$5$PBB0$PBB.5$PBB.0.5$D5.0$321$9$Soil.NH4.63@DNA64$CGTACTAGCTCTCTAT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBB$300$5$PBB300$PBB.5$PBB.300.5$D5.300$125$10$Soil.NH4.64@DNA65$CGTACTAGTATCCTCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBB$300$5$PBB300$PBB.5$PBB.300.5$D5.300$225$11$Soil.NH4.65@DNA66$CGTACTAGAGAGTAGA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBB$300$5$PBB300$PBB.5$PBB.300.5$D5.300$325$12$Soil.NH4.66@DNA67$CGTACTAGGTAAGGAG$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBC$0$5$PBC0$PBC.5$PBC.0.5$D5.0$151$13$Soil.NH4.67@DNA68$CGTACTAGACTGCATA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBC$0$5$PBC0$PBC.5$PBC.0.5$D5.0$251$14$Soil.NH4.68@DNA69$CGTACTAGAAGGAGTA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBC$0$5$PBC0$PBC.5$PBC.0.5$D5.0$351$15$Soil.NH4.69@DNA70$CGTACTAGCTAAGCCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBC$300$5$PBC300$PBC.5$PBC.300.5$D5.300$155$16$Soil.NH4.70@DNA71$AGGCAGAATAGATCGC$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBC$300$5$PBC300$PBC.5$PBC.300.5$D5.300$255$17$Soil.NH4.71@DNA72$AGGCAGAACTCTCTAT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBC$300$5$PBC300$PBC.5$PBC.300.5$D5.300$355$18$Soil.NH4.72