GSM4610716: AA-E316-Jurkat_WT; Homo sapiens; ChIP-Seq
We extracted nuclear fractions and incubated with Mnase (1.5 U μL-1 ) before isolating nuclear complexes with antibody. We used the Diagenode MicroPlex kit for Illumina platforms (Diagenode, #C05010012) following manufacturer's instructions for library preparation. We quantified the libraries using the KAPA Library Quantification Kit for Illumina Platforms (KapaBiosystems, #KR0405) and AMPure XP (Beckman, # A63880) for library purification. We sequenced amplicon pools in an Illumina NextSeq500/505.
GEO Accession
GSM4610716