GSM2650233: clsy3-1_rep2_mRNA-seq; Arabidopsis thaliana; RNA-Seq
4 un-opened flower buds (stage 12 and younger) were collected from the same individual plants as used for the smRNA-seq and MethylC-seq analyses and total RNA was isolated using the Quick-RNA MiniPrep kit (Zymo Research, Cat# R1055). 2.0μg of total RNA from each genotype was used to generate mRNA-seq libraries using NEBNext Ultra RNA Library Prep Kit (NEB, Cat# E7530). Sera-Mag Magnetic SpeedBeads (Thermo scientific, Cat# 65152105050250) were used for all the size selection and clean-up steps. The resulting libraries were equally pooled and sequenced with in SE50 mode using a HiSeq2500 machine.
GEO Accession
GSM2650233