LAC RPMI + 10% LB 0.1ug/mL Nafcillin 2b, 2 hours
MRSA isolate USA300 LAC was exposed to sub-inhibitory concentrations of antibioticsnafcillin in different media (CAMHB or RPMI + 10% LB (R10LB)) for 2 hours and their transcriptomic profile measured with RNAseq.Zymo Research Fungal/Bacterial RNA prep kit was used to isolate and purify the RNA. The cells were lysed using a Roche MagnaLyzer instrument. RNA quality was checked with an Agilent Bioanalyzer instrument and ribosomal RNA was removed using an Illumina Ribo-Zero kit.The final sample enriched for mRNA was usedin the first step of the Kapa Biosystems RNAseq library prep kit. The experiments  were repeated three times generating biological replicates (e.g.1,2,3) and each sample was sequenced two times as technical replicates e.g.(a,b,c).