GSM3601991: 24h MC58 GFP +; Homo sapiens; Neisseria meningitidis MC58; RNA-Seq
RNA was precipitated through the TRIzol method followed by DNase treatment. Total RNA was precipitated by phenol-chloroform extraction followed by ethanol precipitation. The libraries were generated by Vertis Biotechnologie AG (Munich, Germany). The samples were poly(A)-tailed by using poly(A) polymerase.  First-strand cDNA synthesis was performed with an oligo(dT)25 primer. The cDNA was purified using the Agencourt AMPure XP kit (Beckman Coulter Genomics) and was analyzed by capillary electrophoresis. The primers used for PCR amplification were designed for TruSeq sequencing according to the instructions of Illumina.
GEO Accession
GSM3601991