WGS of Pakistani descent
Approximately, 1.0  g of genomic DNA (gDNA) was fragmented using an E210 Focused-ultrasonicator (Covaris, Woburn, MA) and was used to prepare paired-end libraries with the TruSeq DNA PCR-Free Library Preparation Kit (Illumina Inc., San Diego, CA), with an average insert size of 450 bp for both samples (J-1 and K-2). Each library was sequenced in two independent lanes of a HiSeq 2500 in paired-end fashion (2 x 100 bp).