GSM2719085: Cry_KO2_Liver_ZT10_male; Mus musculus; RNA-Seq
Liver RNAs were extracted as previously described (Gachon et al., 2006) with classical methods using guanidium thiocyanate lysis buffer. Libraries were prepared using the TruSeq Stranded Total RNA Sample Prep Kit with the Ribo-Zero Gold depletion set (Illumina) following the manufacturer's protocol. We used 250 ng total RNA as starting material and performed 11 PCR cycles from library amplification (optimal cycle determined with the same method as the small RNA, see above). Samples were pooled by 24, denatured, spiked with 3% PhiX and loaded onto a paired-end read flow cell v3 at a final concentration of 11 pM for a paired-end 100 cycles run, strictly following Illumina's recommendations. Sequencing depth was equivalent to 3 samples per lane.
GEO Accession
GSM2719085