GSM1251749: SMA_MNs rep2; Mus musculus; RNA-Seq
lumbar spinal cord was dissected, embedded in NEG 50 (Richard-Allan Scientific) on dry ice/ethanol mixture. Cross-sections of 8 μm thickness were prepared in a cryostat and transferred to PEN membrane slides (Zeiss). Slides were stained by Mayer’s hematoxylin (Sigma), using a LCM staining kit (Ambion). Laser-capture microdissection (LCM) was performed on a PALM microbeam UV laser microdissection system (Zeiss). Total RNA from LCM samples was purified by using RNeasy Plus Micro kit (Qiagen). ds-cDNA was produced by using Ovation RNA-seq System V2 (NuGEN) and fragmented by a Covaris S-series System (Covaris). DNA fragments in 150-300 bp size range were recovered to construct sequencing library, using Encore NGS Library System I (NuGEN)
GEO Accession
GSM1251749