GSM3380504: Heart_4; Mus musculus; ncRNA-Seq
Upon collection, tissue samples were submerged and preserved at -80C in RNAlater stabilization solution (ThermoFisher cat # AM7021) until further processing. Total RNA was isolated from ~ 100 mg of tissue using Qiagen miRNeasy mini kit (cat # 217004) and the Qiagen tissue lyser using 5 mm stainless steel beads. RNA integrity was assesses using Agilent Bioanalyzer using RNA 6000 pico kit (Agilent Technologies cat # 5067-1513). Short RNA libraries were prepared following the Illumina TruSeq Small RNA Library Preparation kit (cat # RS-200-0012, RS-200-0024, RS-200-0036, RS-200-0048) according to the manufacturer's protocol and size-selected using Pippin Prep 3% Agarose Gel Cassette (Safe Science) in a range 135 bp – 210 bp. Samples were pooled in batches of 48 and sequenced using the Illumina Illumina NextSeq 500 instrument in a single-read, 75-base mode. Short RNA libraries were prepared following the Illumina TruSeq Small RNA Library Preparation kit (cat # RS-200-0012, RS-200-0024, RS-200-0036, RS-200-0048) according to the manufacturer's protocol and size-selected using Pippin Prep 3% Agarose Gel Cassette (Safe Science) in a range 135 bp – 210 bp. Samples were pooled in batches of 48 and sequenced using the Illumina Illumina NextSeq 500 instrument in a single-read, 75 -base mode.
GEO Accession
GSM3380504