Human oral metagenome isolated from healthy and caries children
Approximately 2 ml saliva was collected from children with deep dentin caries (n = 23) and healthy children (n = 24) by spitting method. Saliva was centrifuged at 2,600 g for 10 min to separate the bacterial cells from debris and eukaryotic cells. DNA was extracted and purified from the supernatant by using QIAmp microbiome (Qiagen) and DNA clean & concentrator (Zymo Research) kit procedure. Libraries were prepared using Illuminas Nextera DNA kit according to the manufacturer's instructions. Libraries were sequenced with 2_150 bp paired-end (PE) on an Illumina NextSeq 500 platform at the J. Craig Venter Institute Joint Technology Center.