GSM1923375: Gly15m_RNAseq_12; Saccharomyces cerevisiae; RNA-Seq
sample processing: Total RNA was isolated by phenol/chloroform extraction from thawed whole cell lysate after SDS solubilization and Proteinase K treatment. rRNA was depleted with Ribo-Zero Gold kit for yeast. Remaining RNA was randomly fragmented by alkaline hydrolysis and 30-70 nt fragments were gel purified. Gel-purified RNA was treated with PNK and ligated to a pre-adenylated adapter using T4 RNA ligase 2, K227Q, truncated. Superscript III was used for reverse transcription and circ ligase for circularization. Illumina adapters were introduced by 10-14 cycles of PCR.
GEO Accession
GSM1923375