GSM1245030: brat adult heads_replicate 3; Drosophila melanogaster; RNA-Seq
Total RNA was isolated from adult fly heads by TRIzol purification (Invitrogen) Total RNA(~1µg) was enriched for polyA mRNA (Dynabeads, Invitrogen), fragmented and first strand and second strand (dUTP, instead dTTP was used) was generated. Library preparation was done using a modified protocol from Illumina with NEBNext DNA sample Prep Reagent kits (NEB), and UGDase treatment was done to confer strand specificity.
GEO Accession
GSM1245030