GSM2214207: 15A-96-R1; Fusarium graminearum; RNA-Seq
Total RNA was extracted using Promega SV total RNA isolation kit. Extracted RNA was converted dscDNA and used for library preparation and sequencing using TruSeq RNA sample preparation kit from Illumina. The library was constructed using TruSeq RNA sample preparation kit (Illumina, San Diego, CA) according to the manufacturer’s protocol. Briefly, the poly-A containing mRNA was purified from the total RNA using the poly-T oligo-attached to the magnetic beads. After purifications, mRNA was fragmented into small pieces using divalent cations under elevated temperature. The fragmented mRNA was converted to first strand cDNA using reverse transcriptase and random primers. Single strand cDNA was further converted to double strand (ds) cDNA using DNA polymerase I and RNase H. Then, the ds cDNA fragments were end repaired, ligated with indexing adapters, purified and enriched with PCR to develop the library. The prepared libraries were sent to Huntsman Cancer Institute, the University of Utah (Salt Lake City, UT) for generating 50bp single-end reads with the Illumina HiSeq 2000 sequencing system
GEO Accession
GSM2214207