high-throughput sequencing of prokaryote: bacteria and archaea in coastal salar saltern
The sequence primers were corresponding to the V3-4 region of bacterial 16S rRNA gene.. The sequence technology of Solexa was used which was sequencing by synthesis. The original data was firstly tested with Fastqc software to remove the adaptor and low-quality base sequences, and the raw samples were read and counted. Then the double-ended reads were linked together according to overlapping bases. Finally, the valid sequences were obtained and performed subsequent bioinformation analysis.