Heads of pea aphid clone LSR1 reared on Vicia faba  replicate 3
Aphids were flash frozen by liquid nitrogen and heads were cut by scalpel while whole aphid body was frozen. On average, 20 aphid heads harvested on the same day were pooled for one replicate. RNA from SGs, ATs and heads were extracted by NucleoSpin RNA XS (Macherey-Nagel) and quantified. rRNA depletion, single stranded-RNA library preparation, multiplexing, and sequencing were performed by Genewiz (New Jersey, USA).