microRNA of kidney
The small RNAs fraction were isolated by polyacrylamide gel electrophoresis and ligated with proprietary adaptors (Illumina, USA). Short RNAs were then reverse-transcribed into cDNA by RT-PCR. The four small RNA libraries were constructed for hybird snakehead and then sent to LC-Sciences (Hangzhou, China) for deep sequencing using an Illumina Hiseq 2500 platform