GSM1684594: Nannochloropsis oceanica (IMET1, log phase); Nannochloropsis oceanica; OTHER
N. oceanica IMET1 was cultivated in f/2 liquid medium with 4mM NO3- under continuous light at 50 micromol photons m^(-2)s^(-1) until mid-log phase. Culture was pelleted and nucleic acid was extracted using phenol-chlorophorm and treated with RNase to degrade RNA. 5-10ug of gDNA was sheared to >10kb using g-tube (Covaris). The sheared DNA was treated with DNA damage repair mix followed by end repair and ligation of SMRT hairpin adapters using SMRTbell Template Preparation Reagent Kit (Pacific Biosciences). Fragments without adaptors are digested with exonuclease. Libraries were sequenced on Pacific Biosciences RSII sequencer using standard protocols.
GEO Accession
GSM1684594