GSM4273439: GFP+ replicate 1; Danio rerio; RNA-Seq
About 1500 6hpf Tg(krt18:krt18-GFP) embryos were dechorionated in pronase 1mg/ml in danieu solution. Embryos were collected, vortexed 3x 30 sec to prepare a cell suspension, cells centrifuged at 200g 3 min, resuspended in DMEM, and filtered with a 40um filter. 1x10^4 GFP+ cells were sortedand RNA was prepared using the RNeasy Micro Kit Illumina libraries were prepared using the SMARTseq2 protocol and sequenced on a HiSeq2500 v4 to produce 50-nt single end reads
GEO Accession
GSM4273439