GSM1678988: Exosomes, replicate #2; Mus musculus; miRNA-Seq
Total RNA was isolated using TRIzol reagent (Life Technologies) following the manufacturer’s recommendations. In short, an adenylated adapter was ligated to the 3’ end of the total RNA by a truncated T4 RNA Ligase 2, followed by the ligation of a 5’ RNA-adapter by T4 RNA Ligase 1 (NEB). After reverse-transcription using a specific primer, the cDNA was amplified by PCR. After gel-fractionation bands corresponding to miRNA inserts were cut out. PCR products from these bands were eluted in elution buffer, precipitated and dissolved in water. The quality of the libraries was assessed by qPCR and Bioanalyzer measurements.
GEO Accession
GSM1678988