GSM4118087: PLT Control; Homo sapiens; miRNA-Seq
Total RNA was isolated using Trizol LS and suspended in DEPC-treated DNase-RNase-free water prior to RNA purification and subsequent on-column DNase treatment using RNeasy mini-kit following the manufacturer protocol. Total RNA was used to prepare the microRNA sequencing library, which included the following steps: 1) 3'-adapter ligation; 2) 5'-adapter ligation; 3) cDNA synthesis; 4) PCR amplification; and 5) size selection of PCR amplified fragments of  ~130–150 base pairs (corresponding to small RNAs of ~15–35 nucleotides). The complete libraries were quantified by Agilent 2100 Bioanalyzer.
GEO Accession
GSM4118087