GSM2124001: ZEB_50; Arabidopsis thaliana; Bisulfite-Seq
Tissue was flash frozen in liquid nitrogen for all experiments including RNA-seq and MethylC-seq. DNA was isolated using a Qiagen Plant DNeasy kit (Qiagen, Valencia, CA) following the manufacturer's recommendations. RNA was isolated using TRIzol (Thermo Scientific, Waltham, MA) following the manufacturer's instructions. RNA-seq: RNA-seq libraries were constructed using Illumina TruSeq Stranded RNA LT Kit (Illumina, San Diego, CA) following the manufacturer's instructions with limited modifications. The starting quantity of total RNA was adjusted to 1.3 µg, and all volumes were reduced to a third of the described quantity. MethylC-seq libraries were constructed using the MethylC-seq protocol (Urich et al. 2015).
GEO Accession
GSM2124001