GSM3833114: Veh_2wk_3; Homo sapiens; OTHER
DNA was extracted from frozen cultured cells and xenografts using the AllPrep DNA/RNA Mini Kit or the QIAamp DNA Mini Kit (Qiagen). PCR was used to amplify barcodes and introduce Illumina adaptors along with a 5 bp index sequence for multiplexing as described (Bhang et al., 2015). 2 µg of genomic DNA was used as template, and 15-16 samples were multiplexed. PCR products were run on 0.8% agarose NGS E-Gels (Invitrogen) to verify the correct library size, and bands were purified using the MinElute Gel Extraction Kit (Qiagen).