Ammonia amended soil from 56-days aerobic dynamic: day 7
This paired-end SRA file contains the V1-V3 16S rRNA raw sequences as a multiplexed library from the 18 ammonia amended soil samples for Day 7 of the 56-days aerobic dynamic. The paired-end fastq files must be fed into the split_libraries_fastq.py script to demultiplex each Sample by its 16-bp unique barcode. The mapping file to be used is the following; where '$' must be changed to tabulation ('\t') and '@' to a new line ('\n'): SampleID$BarcodeSequence$LinkerPrimerSequence$ReversePrimer$soil_use$dinamic$Practice$Fertilizer$Day$Treatment$Pract.Day$Pract.Fertil.Day$Day.Fertilizer$Soil$OH$Description@DNA73$TAAGGCGATAGATCGC$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$amended$CT$0$7$CT0$CT.7$CT.0.7$D7.0$111$1$Soil.NH4.73@DNA74$TAAGGCGACTCTCTAT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$amended$CT$0$7$CT0$CT.7$CT.0.7$D7.0$211$2$Soil.NH4.74@DNA75$TAAGGCGATATCCTCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$amended$CT$0$7$CT0$CT.7$CT.0.7$D7.0$311$3$Soil.NH4.75@DNA76$TAAGGCGAAGAGTAGA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$amended$CT$300$7$CT300$CT.7$CT.300.7$D7.300$115$4$Soil.NH4.76@DNA77$TAAGGCGAGTAAGGAG$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$amended$CT$300$7$CT300$CT.7$CT.300.7$D7.300$215$5$Soil.NH4.77@DNA78$TAAGGCGAACTGCATA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$amended$CT$300$7$CT300$CT.7$CT.300.7$D7.300$315$6$Soil.NH4.78@DNA79$TAAGGCGAAAGGAGTA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBB$0$7$PBB0$PBB.7$PBB.0.7$D7.0$121$7$Soil.NH4.79@DNA80$TAAGGCGACTAAGCCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBB$0$7$PBB0$PBB.7$PBB.0.7$D7.0$221$8$Soil.NH4.80@DNA81$CGTACTAGTAGATCGC$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBB$0$7$PBB0$PBB.7$PBB.0.7$D7.0$321$9$Soil.NH4.81@DNA82$CGTACTAGCTCTCTAT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBB$300$7$PBB300$PBB.7$PBB.300.7$D7.300$125$10$Soil.NH4.82@DNA83$CGTACTAGTATCCTCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBB$300$7$PBB300$PBB.7$PBB.300.7$D7.300$225$11$Soil.NH4.83@DNA84$CGTACTAGAGAGTAGA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBB$300$7$PBB300$PBB.7$PBB.300.7$D7.300$325$12$Soil.NH4.84@DNA85$CGTACTAGGTAAGGAG$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBC$0$7$PBC0$PBC.7$PBC.0.7$D7.0$151$13$Soil.NH4.85@DNA86$CGTACTAGACTGCATA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBC$0$7$PBC0$PBC.7$PBC.0.7$D7.0$251$14$Soil.NH4.86@DNA87$CGTACTAGAAGGAGTA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBC$0$7$PBC0$PBC.7$PBC.0.7$D7.0$351$15$Soil.NH4.87@DNA88$CGTACTAGCTAAGCCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBC$300$7$PBC300$PBC.7$PBC.300.7$D7.300$155$16$Soil.NH4.88@DNA89$AGGCAGAATAGATCGC$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBC$300$7$PBC300$PBC.7$PBC.300.7$D7.300$255$17$Soil.NH4.89@DNA90$AGGCAGAACTCTCTAT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBC$300$7$PBC300$PBC.7$PBC.300.7$D7.300$355$18$Soil.NH4.90