Illumina HiSeq 4000 sequencing; Bulk RNA sequencing of colorectal tumors for molecular subtyping
Bulk RNA sequencing of colorectal tumors for molecular subtyping
Resection samples were cut open and rinsed with water to remove any remaining stool. Two samples from each tumor specimen were collected corresponding to tumor core and invasive margin (tumor border). The samples were then snap frozen in liquid nitrogen and subsequently stored in -80oC for long term storage. RNA isolation was performed with Trizol (15596026, Invitrogen) using the protocol recommended by the manufacturer. For nucleic acid library construction, nKAPA Stranded mRNA-Seq Kit (KR0960 – v5.17, KAPABiosystems) was used according to the manufacturer recommendations.
Experimental Factor: individual
SC030
Experimental Factor: sampling site
tumor border