WGS_Mycobacterium_bovis_SP38
Extracted DNA was sent to the Genomics Core Facility of Purdue University, USA for sequencing. Briefly, the quality and concentration of the DNA were measure by Nanodrop 2000C (Thermo Fischer Scientific, Massachusetts, USA) and 0.8% agarose gel using a mass ladder. DNA was sheared into 800 bp (base pairs) fragments using the Hydroshear DNA Shearing Device (Genomic Solutions, Michigan, USA) to create fragments for paired end libraries. Sheared DNA was analyzed using a DNA High Sensitivity Chip in an Agilent 2100 Bioanalyzer (Agilent Technologies, California, USA), resulting in average fragment size of 804 bp. A paired-end genomic library was then constructed using TruSeq DNA PCR-free sample preparation kit (Illumina, California, USA) and 20% of a lane (Illumina v3 chemistry) of Illumina HiSeq2500 was used to sequence it.