GSM3231047: M2B_WT_SELEXseq_R0; Homo sapiens; OTHER
Protein-DNA binding reactions were loaded onto a polyacrylamide gel and subject to electrophoretic mobility assay (EMSA), allowing for separation of bound and unbound fragments. Bound fragments were isolated from the gel and subject to phenol:chloroform extraction for DNA extraction, followed by ethanol precipitation. Libraries were prepared for sequencing based on the Illumina TruSeq small RNA kit sequences. The DNA library 5'-GAGTTCTACAGTCCGACGATCCGC[N16]CCTGGAATTCTCGGGTGCCA-3' containing  fixed regions compatible with Illumina sequencing was used in a 4-cycle PCR with primers RP1 5'AATGATACGGCGACCACCGAGATCTACACGTTCAGAGTTCTACAGTCCGA  and RPI# 5'CAAGCAGAAGACGGCATACGAGAT[Index]GTGACTGGAGTTCCTTGGCACCCGAGAATTCCA to add final Illumina adapter regions and indexes for sample multiplexing.
GEO Accession
GSM3231047