GSM1245129: rdr2 (dsRNA); Physcomitrella patens; RNA-Seq
Total RNA was extracted with Tri-Reagent (Sigma) per the manufacturer's instructions dsRNAseq libraries for the Illumina GAIIx were prepared as described by Zheng et al. (2010) (PMID: 20941385). Brief method [excerpted from Zheng et al.]: Total RNA is subjected to one (1X Ribominus) or two (2X Ribominus) rounds of rRNA depletion as per manufacturer’s instructions (Ribominus, Invitrogen (Carlsbad, CA)). Next, these rRNA-depleted RNA samples are treated with a single-strand specific ribonuclease as per manufacturer’s instructions (RNase One, Promega (Madison, WI)). The RNA sample is then used as the substrate for sequencing library construction using the Small RNA Sample Prep v1.5 kit (Illumina, San Diego, CA) as per manufacturer’s instructions.
GEO Accession
GSM1245129