Deep Sequencing of sncRNAs from Cochliomyia macellaria Larvae
Small RNA library construction was prepared using Illumina TruSeq Small RNA Preparation Kit 2 (Illumina Inc., San Diego, CA, USA) according to manufactures instructions. Sequencing librarie was enriched for sncRNAs via PCR amplification using cDNA as template and subsequently sequenced-by-synthesis in multiplex 1x50bp Illumina MiSeq system.