GSM1681976: U251 WT CLIP-seq Rep 2; Homo sapiens; RIP-Seq
iCLIP was performed according to Konig et al using Abcam rabbit anti-MSI1 monoclonal antibody (ab52865) (Abcam, Cambridge, MA). Samples were amplified, purified with Beckman Genomics Ampure XP beads (Beckman Coulter Genomics, Danvers, MA), and quantitated with an Agilent 2100 Bioanalyzer and DNA 1000 chips (Agilent Technologies, Santa Clara, CA). Three biologic replicates were combined and 50SE sequencing performed on an Illumina Hi-Seq 2000 (Illumina, Inc., San Diego, CA).
GEO Accession
GSM1681976