GSM4273202: f3_str_GFP-; Mus musculus; ATAC-seq
GFP positive and negative cells were isolated by FACS using a FACSAria flow cytometer (BD Biosciences). ATAC-seq reactions were performed using an established protocol (Buenrostro et al PMID: 25559105) (Illumina Cat #FC-121-1030) and the resulting libraries generated using the Nextera index kit (Illumina Cat #FC-121-1011). Libraries were amplified for a total of 14–16 cycles of PCR. Following PCR, ATACseq libraries were resolved on 2% agarose gels and fragments ranging in size from 100bp-1Kbp were excised and purified (Qiagen Minelute Gel Extraction Kit – Qiagen Cat#28604). Libraries were quantified by Qubit HS DNA kit (Life technologies) and by quantitiative PCR (KAPA Biosystems Cat#KK4873) prior to sequencing. Finally, ATACseq libraries were sequenced on Hi-Seq2500 (Illumina) obtaining 2x50 paired-end reads.
GEO Accession
GSM4273202