GSM2990222: YAP-OE -DOX rep1; Mus musculus; RNA-Seq
Livers were perfused with a PBS and collagenase mixture through the inferior vena cava, minced with a razor blade, and passed through a 40 µm strainer. Hepatocytes were separated by low-speed centrifugation (50 g x 30 s) and washed with PBS. Total RNA from hepatocytes was extracted using RNeasy Mini Kit (Qiagen). RNA quality was assessed on the BioAnalyzer (Agilent Technologies). Qualifying samples were prepared following the standard protocol for the TruSeq stranded mRNA library kit (Illumina) on the Illumina Neoprep automated nanofluidic library prep instrument. Triplicate libraries were sequenced on the Illumina NextSeq 500.
GEO Accession
GSM2990222