GSM1684592: Auricularia delicata (TFB10046 SS-5); Auricularia delicata; OTHER
Auricularia delicata, strain TFB10046, SS-5 was prepared as previously described in Floudas, D. et al. Science 336, 1715-1719 (2012). 5-10ug of gDNA was sheared to >10kb using g-tube (Covaris). The sheared DNA was treated with DNA damage repair mix followed by end repair and ligation of SMRT hairpin adapters using SMRTbell Template Preparation Reagent Kit (Pacific Biosciences). Fragments without adaptors are digested with exonuclease. Libraries were sequenced on Pacific Biosciences RSI sequencer using standard protocols.
GEO Accession
GSM1684592