GSM3832712: Nogo_KO_1; Mus musculus; RNA-Seq
Total RNA was isolated from the gastrocnemius muscle tissues of WT and Nogo KO mice (3 biological replicates per each group) using Qiagen RNeasy mini Kit. mRNA was then isolated from the total RNA and fragmented using Illumina Truseq™ Stranded mRNA LT Sample Prep Kit with poly-T oligo magnetic beads, and reverse transcription of the mRNA fragments was conducted using Superscript III reverse transcriptase (Life Technologies). Adaptor ligated libraries were generated according to the manufacture's standard protocol and sequenced using HiSeq 2500 system.
GEO Accession
GSM3832712