Whole Genome Analysis of Diverse Sequence Types Advances Our Understanding of Population Genetics and Evolution of Staphylococcus aureus
Mate pair libraries were prepared using the manufacturer’s protocol (Life Technologies Corp). Sheared, methylated chromosomal DNA was prepared, adaptors were ligated, and the fragments were circularized. Digestion with EcoP151 resulted in 25-base pair tags separated by a distance corresponding to the initial library fragment size. After ligation of sequencing adaptors, library fragments were amplified and attached to beads by emulsion PCR, and the 25-base pair tags were sequenced.