dsDNA Illumina HiSeq of a polar lake virome
Libraries were built with NEBNext Ultra II kit for Illumina using  provided adapters (paired-end) . Quality was assessed on a Agilent bioanalyzer to check for adapters duplex which were removed with Agencourt AMPure XP magnetic beads when needed. Sequencing was done on HiSeq 2500 at Genome Quebec (Montreal, Qc, Canada). Samples AII15 and AII40 were resequenced the same way as the first sequencing did not yield a satisfying amount of sequences.