GSM1679356: Hyphae C footprint; Histoplasma capsulatum; OTHER
Hyphal and yeast cells were treated with 100 μg/mL cycloheximide for 2 minutes (MP Biomedicals, Santa Ana, CA) before harvesting of cells by filtration. Total RNA for mRNA library preparation was isolated from a small fraction of the total yeast or hyphal cells collected for ribosome profiling using a guanidine thiocyanate lysis protocol as previously described (Hwang et al, Molecular Biology of the Cell 14:2314). Ribosome profiling and matched mRNA-seq sample preparation and library building were performed as described (Ingolia et al Science 324:218), except that the 3′ linker ligation strategy was used instead of poly(A) tailing for marking and capturing the 3’ RNA end (Dunn et al eLife 2:e01179). Libraries were multiplexed and subjected to 50 bp single-end sequencing with an Illumina HiSeq2000 sequencer (Illumina).
GEO Accession
GSM1679356