Genome sequencing reads from individual field collected Anopheles arabiensisfrom the village of Lupiro in Tanzania
We used 25-50ng of input DNA from single mosquito for library construction. DNA was then cleaned and concentrated with the DNA Clean and Concentrator kit (Zymo Research Corporation). Library preparations were made with the Nextera DNA Sample Preparation Kit (Illumina), using TruSeq dual indexing barcodes (Illumina). Libraries were size-selected with Agencourt AMPure XP beads (Beckman Coulter). We assessed the insert size distribution of the final libraries using a QIAxcel instrument (Qiagen, Valencia, CA) or Bioanalyzer 2100 (Agilent), and the final library concentration was measured with a Qubit 2.0 fluorometer (Life Technologies).