16S DNA and RNA was extracted from cryoconite, snow and stream water then processed for sequencing using Illumina MiSeq specified protocols. Sequence output was demultiplexed, chimera checked and reference OTUs picked in QIIME before downstream analysis and interpretation.
16S DNA and RNA was extracted from cryoconite, snow and stream water then processed for sequencing using Illumina MiSeq specified protocols. Sequence output was demultiplexed, chimera checked and reference OTUs picked in QIIME before downstream analysis and interpretation.