ANPacBio_36M
We extracted total genomic DNA from 36 A. nasatum brothers using the Qiagen DNeasy Blood and Tissue kit, according to the protocol for animal tissues excluding digestif tract and cuticle (3 h incubation in proteinase-K at 56C and 30 min RNAse treatment at 37C). These males are from our inbred laboratory lines derived from wild animals sampled Thur (France). 50 SMRT sequencing libraries were prepared (from pooled DNA) and sequenced as 10-52000pb.