GSM3380553: mESC CTCF PLAC-seq, biological replicate 2; Mus musculus; OTHER
In brief, 1-3 million crosslinked F123 cells were digested 2 hours at 37oC using 100 U of MboI followed by biotin fill-in and proximity ligation at room temperature for 4 hours. Then the nuclei were further lysed, sonicated and immunoprecipitated against the antibodies of choice. After immunoprecipitation, reverse crosslink was performed overnight at 65 oC after adding proteinase K to extract DNA. DNA fragments containing ligation junctions were enriched with streptavidin beads followed by on-beads end repair, A-tail adding, adapter ligation and PCR amplification for 12-13 cycles.
GEO Accession
GSM3380553