Transcriptome of needles using Illumina Genome Analyzer IIx
The full-length cDNA library was constructed essentially as reported previously (Seki et al. (1998) Plant J. 15:707-720; Seki et al. (2002) Science 296:141-145).  The cDNAs derived from needles of C. japonica are inserted into a modified pBluescript vector.  Plasmid DNA was digested by PI-Sce I whose site is placed near the 3'-end of a cDNA insert and then sense strand RNA was synthesized by RNA polymerase.  The RNA-seq library was constructed using this synthesized RNA.