GSM3380706: UACC-812_input; Homo sapiens; ChIP-Seq
Chromatin extrated cell cell lysate was sonicated and shered to 300bp to 1kb fragments using a Sonifier Cell Disruptor (Branson Ultrasonics #101-063-588). To 0.5ml sheared chromatin (after clarification of debrie), we added 150µl ChIP mix (440mM NaCl, 0.44% sodium deoxycholate, 4.4% Triton X-100) and antibody-coupled beads for immunoprecipitation overnight at 4C. Precipitated DNA fragments were processed to ChIP-seq library using the ThruPLEX DNA-seq 12S kit (Takara #R400428) according to manufacturer's instructions. 75bp single end 10M depth
GEO Accession
GSM3380706