GSM1252084: col BS-seq; Arabidopsis thaliana; Bisulfite-Seq
Genomic DNA was extracted with CTAB method from 14-d Arabidopsis seedlings. Then RNA is eliminated by RNaseI. Five micrograms of genomic DNA was fragmented into 100-300bp by sonication approach.  The fragments were blunt‐ended and phosphorylated, and a single ʹAʹ nucleotide was added to the 3ʹ ends of the fragments. Then the fragments were ligated to adaptors. The resulting DNAs were treated with ZYMO EZ DNA Methylation-Gold kit to finish bisulfite conversion. After DNAs were recovered from gel, PCR amplifications were performed to enrich those DNAs with proper sizes. Finally, the final purified products were quantitated prior to cluster generation.
GEO Accession
GSM1252084