Ammonia unamended soil from 56-days aerobic dynamic: day 3
This paired-end SRA file contains the V1-V3 16S rRNA raw sequences as a multiplexed library from the 18 ammonia unamended soil samples for Day 3 of the 56-days aerobic dynamic. The paired-end fastq files must be fed into the split_libraries_fastq.py script to demultiplex each Sample by its 16-bp unique barcode. The mapping file to be used is the following; where '$' must be changed to tabulation ('\t') and '@' to a new line ('\n'): SampleID$BarcodeSequence$LinkerPrimerSequence$ReversePrimer$soil_use$dinamic$Practice$Fertilizer$Day$Treatment$Pract.Day$Pract.Fertil.Day$Day.Fertilizer$Soil$OH$Description@DNA181$TAAGGCGATAGATCGC$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$unamended$CT$0$3$CT0$CT.3$CT.0.3$D3.0$111$1$Soil.CTRL.37@DNA182$TAAGGCGACTCTCTAT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$unamended$CT$0$3$CT0$CT.3$CT.0.3$D3.0$211$2$Soil.CTRL.38@DNA183$TAAGGCGATATCCTCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$unamended$CT$0$3$CT0$CT.3$CT.0.3$D3.0$311$3$Soil.CTRL.39@DNA184$TAAGGCGAAGAGTAGA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$unamended$CT$300$3$CT300$CT.3$CT.300.3$D3.300$115$4$Soil.CTRL.40@DNA185$TAAGGCGAGTAAGGAG$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$unamended$CT$300$3$CT300$CT.3$CT.300.3$D3.300$215$5$Soil.CTRL.41@DNA186$TAAGGCGAACTGCATA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$unamended$CT$300$3$CT300$CT.3$CT.300.3$D3.300$315$6$Soil.CTRL.42@DNA187$TAAGGCGAAAGGAGTA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBB$0$3$PBB0$PBB.3$PBB.0.3$D3.0$121$7$Soil.CTRL.43@DNA188$TAAGGCGACTAAGCCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBB$0$3$PBB0$PBB.3$PBB.0.3$D3.0$221$8$Soil.CTRL.44@DNA189$CGTACTAGTAGATCGC$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBB$0$3$PBB0$PBB.3$PBB.0.3$D3.0$321$9$Soil.CTRL.45@DNA190$CGTACTAGCTCTCTAT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBB$300$3$PBB300$PBB.3$PBB.300.3$D3.300$125$10$Soil.CTRL.46@DNA191$CGTACTAGTATCCTCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBB$300$3$PBB300$PBB.3$PBB.300.3$D3.300$225$11$Soil.CTRL.47@DNA192$CGTACTAGAGAGTAGA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBB$300$3$PBB300$PBB.3$PBB.300.3$D3.300$325$12$Soil.CTRL.48@DNA193$CGTACTAGGTAAGGAG$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBC$0$3$PBC0$PBC.3$PBC.0.3$D3.0$151$13$Soil.CTRL.49@DNA194$CGTACTAGACTGCATA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBC$0$3$PBC0$PBC.3$PBC.0.3$D3.0$251$14$Soil.CTRL.50@DNA195$CGTACTAGAAGGAGTA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBC$0$3$PBC0$PBC.3$PBC.0.3$D3.0$351$15$Soil.CTRL.51@DNA196$CGTACTAGCTAAGCCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBC$300$3$PBC300$PBC.3$PBC.300.3$D3.300$155$16$Soil.CTRL.52@DNA197$AGGCAGAATAGATCGC$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBC$300$3$PBC300$PBC.3$PBC.300.3$D3.300$255$17$Soil.CTRL.53@DNA198$AGGCAGAACTCTCTAT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBC$300$3$PBC300$PBC.3$PBC.300.3$D3.300$355$18$Soil.CTRL.54