GSM2813132: C49R (Exome-Seq); Homo sapiens; OTHER
Genomic DNA was extracted from the frozen cell populations with a QIAamp DNA Mini Kit (Qiagen). Sequencing libraries were prepared as previously described (Brastianos et al., 2013). Briefly, gDNA from five cell lines and one human CEPH normal (http://hapmap.ncbi.nlm.nih.gov/citinghapmap.html.en) were fragmented to 250 bp using Adaptive Focused Acoustics (AFA) ultra-sonication (Covaris Inc., Woburn, MA) and further purified using Agencourt AMPure XP beads (Beckman Coulter, Inc., Indianapolis, IN). A total of 50 ng of size-selected DNA was ligated to DNA barcoded adaptors during library preparation (KAPA HTP DNA Library Preparation Kit, KK8234, Kapa Biosystems, Inc., Wilmington, MA). Each library was made with sample-specific barcodes and quantified using an Illumina MiSeq Nano flow cell (Illumina Inc., San Diego, CA). For exome enrichment, the 6 libraries were pooled in 3 x 2-plex to a total of 750 ng per pool, and exonic regions were captured with the SureSelect Target Enrichment system using the Human All Exon V5 hybrid capture kit (Agilent Technologies, Santa Clara, CA). All captures were further pooled and sequenced in two lanes of the HiSeq 2500 system in Rapid Run Mode (Illumina Inc., San Diego, CA). Exome-seq
GEO Accession
GSM2813132