Regulation of miRNA host gene expression via alternative polyadenylation
Library construction:  To prepare Poly(A)+ libraries, we started with 500 ng Poly(A)+ RNA from each sample. The RNA was fragmented using RNAse III, followed by ligation of SOLiD(R) adaptors, reverse transcription, and size selection for subsequent amplification, according to the manufacturers’ instructions (Life Technologies). After assessing the amplified DNA for yield and size distribution on the Bioanalyzer instrument (Agilent), libraries were submitted to emulsion PCR followed by sequencing on the SOLiD4 System.  Experimental design: U87MG cell line was transiently transfected with ON-TARGETplus SMARTpool siRNA against CPSF2 gene.