dsDNA Illumina HiSeq of a polar lake virome
Libraries were built with NEBNext Ultra II kit for Illumina using  provided adapters (paired-end) . Quality was assessed on a Agilent bioanalyzer to check for adapters duplex which were removed with Agencourt AMPure XP magnetic beads when needed. Sequencing was done on HiSeq 2500 at Genome Quebec (Montreal, Qc, Canada).