Imperial College : B. dendrobatidis Illumina resequencing project (Hammersmith) - SP10 (IT)
Paired-end Libraries were constructed according to the protocols provided by Illumina sequencing (Truseq kit). DNA was sheared into 150-500bp fragments using a Covaris S2 sonicator. After end-repair and size selection using magnetic beads, barcoded adapters (Illumina 12plex tags) were ligated to the fragments and PCR amplified using 10 cycles. The final libraries were quality-controlled on a bioanalyzer and quantified with qPCR. Two pools of Libraries were used, and sequenced on 2 lanes of an Illumina HiSeq 2000 machine. The output read length was 100bp. Sequence data was processed using RTA version 1.12.4.2, with default filter and quality settings. Sequence files were generated with CASAVA 1.7. Fastq Conversions were performed with a custom script.