Forward end merged Transcriptomic data of all 3 replicates of BHU-22 wheat cultivar
Total RNA of leaves tissue of BHU-22 wheat cultivar was extracted using standard Trizol RNA extraction protocol. The integrity (quality and quantity) of RNA was verified by RNase free agarose gel electrophoresis and the concentration was measured using a 2100 Bioanalyzer [Agilent Technologies, Santa Clara, CA]. High-quality RNA having RNA integrity number (RIN)   8.0 was used for mRNA purification. The mRNA was purified using oligo-dT beads [TruSeq RNA Sample Preparation Kit, Illumina] using 1  g of intact total RNA. The purified mRNA was fragmented at 90 C in presence of divalent cations. The fragments were reverse transcribed using random hexamers and superscript II reverse transcriptase [Life Technologies]. Further cDNA was synthesized on the first strand template using RNase and DNA polymerase I and obtained cDNAs were cleaned using Beckman coulter agencourt ampure XP SPRI beads. These cDNAs were ligated with Illumina adapters after end-repair and the addition of an  A  base followed by SPRI cleanup. The prepared cDNA library was amplified using PCR for the enrichment of the adapter-ligated fragments. The individual libraries were quantified using a NanoDrop spectrophotometer (Thermo Scientific) and validated for quality with a Bioanalyzer. Subsequently, these libraries of each sample were used for RNA sequencing Illumina HiSeq 2500 platform [Illumina Inc., CA, USA].