GSM2052355: Ck2; Citrus trifoliata; RNA-Seq
Total RNA was extracted using TRIzol® (Invitrogen, USA) reagent according to the on-line manuals (http://tools.lifetechnologies.com/content/sfs/manuals/trizol_reagent.pdf). TURBO DNAase reagent kit (Ambion, USA) was used to remove DNA contamination. Total RNA from 6 samples (three biological replicates of mycorrhizal and non-mycorrhizal samples) were used for RNA-seq via an Illumina HiSeqTM2000 at the Beijing Genomics Institute (Shenzhen). Firstly, total RNA from each sample was used to enrich mRNA using the oligo (dT) magnetic beads, then the mRNA was interrupted and used for the synthesis of the double-strand cDNA (Mortazavi et al., 2008). The double-strand cDNA was purified, washed in EB buffer for end repair, added with single nucleotide A (Adenine) and ligated with sequencing adaptors. The fragments obtained were used for PCR amplification, followed by library construction and sequencing. The Agilent 2100 Bioanalyzer and ABI StepOnePlus Real-Time PCR System were used for checking the quality and quantity of the library.
GEO Accession
GSM2052355