Paired-end sequencing of Chrysomela populi's transcriptome and subsequent de novo assembly.
The larvae and adults of Chrysomela populi (various stages) were prepared and the mRNA extracted of gut tissue, glandular tissue, Malpighian tubules and fat body. These mRNA samples were pooled, and a cDNA library was generated. This library was sequenced using a Illumina GAIIx in paired-end mode. All reads were extracted in FastQ format and de novo assembled to reconstruct the transcriptome. Annotation, phylogenetic, RNA-seq and RNAi studies are planned.