GSM2810571: PL-BLA_vTRAP_IP_rep3; Mus musculus; RIP-Seq
RNA was isolated using previously established protocols (Nectow et al., 2017). Briefly, GFPL10-positive polysomes were immunoprecipitated using anti-GFP magnetic beads, followed by RNA purification using Agilent Nanoprep. Libraries were prepared with the SMARTer Ultra Low RNA for Illumina sequencing kit (Clontech), followed by Nextera XT DNA sample preparation kit (Illumina).
GEO Accession
GSM2810571