GSM1604052: WKY KO rep1; Rattus norvegicus; RNA-Seq
Total RNA was extracted from rat (Wild type Wistar-Kyoto strain, n=3; Cyp2j4-/-, n=3) and mice (commercially outbred mice; n=4) bone marrow derived macrophages (BMDMs) and human monocytes-derived macrophages (n=3 healthy donors) using Trizol (Invitrogen) according to manufacturer's instructions with an additional purification step by on-column DNase treatment using the RNase-free DNase Kit (Qiagen) to ensure elimination of any genomic DNA. The integrity and quantity of total RNA was determined using a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific) and Agilent 2100 Bioanalyzer (Agilent Technologies). 1μg of total RNA from each species was used to generate RNA-seq libraries using TruSeq RNA sample preparation kit (Illumina) according to the manufacturer's instructions. Briefly, RNA was purified and fragmented using poly-T oligo-attached magnetic beads using two rounds of purification followed by the first and second cDNA strand synthesis.
GEO Accession
GSM1604052