GSM2811121: ME_JH_112315_1109-ATACSlice03-04-10whole; Drosophila melanogaster; ATAC-seq
Transposed DNA was purified using Qiagen Minelute kit.  Libraries were then amplified using Phusion (NEB cat no. F531S) and Illumina Nextera index kit (cat no. FC-121-1011). Libraries were then purified with Ampure Beads at a 1.2 : 1 beads to sample ratio Libraries were constructed using a modified Illumina Nextera DNA protocol and sequenced on the Hiseq4000 using 100bp paired end reads. Fragments over 500bp were removed from libraries using a Pippen prep to reduce sequencing bias with the Hiseq4000.
GEO Accession
GSM2811121