Crayfish epithelial transcripts
RNA was extracted from the hypodermis and the gastrolith disc of male Cherax quadricarinatus at a variety of molt stages. These stages include intermolt, early and late premolt, and postmolt. The EZ-II extraction kit of Biological Industries, Israel was used for the extraction. The extracted RNAs were further purified using Qiagen RNeasy kit. The various extractions were equally mixed and a total of 10 mg was sent to sequencing after quality examination using bioanalyser (Agilent 2100).