GSM3073148: 361_pos_bulk; Homo sapiens; RNA-Seq
Cells were sorted by HIV-1 RNA SortSeq into bulk (8-30 cells per sample) or single cells into RNA preserving buffer. RNA was reverse crosslinked with proteinase K and SDS under 50°C for 1 hour. RNA was extracted by Quick-RNA Microprep (Zymo Research) with a built-in on-column DNase treatment step. Total RNA was extracted followed by a ribosomal RNA depletion step using the SMARTer Stranded Total RNA-Seq Kit v1 (individuals 21, 108, 159, 361) and v2 (individuals 154, 218, 256, 381) - Pico Input Mammalian (Pico v2). Samples with different barcodes were pooled into one high-output NextSeq 400M 2x150 platform, targeting an estimated reads of 20-40 million per sample.
GEO Accession
GSM3073148