GSM4547028: H3K27me3_ChIPSeq_DMSO; Homo sapiens; ChIP-Seq
Cells were cross-linked for 12 min at room temperature by the addition of one-tenth of the volume of 11% formaldehyde solution to the growth media. DNA was prepared using Agilent Chip-on-chip protocol (G4481-90010). Each immunoprecipitation was performed by incubating 10ug of the corresponding antibody with sheared DNA overnight at 4 degrees C, followed by 2 hour incubation with protein A/G (50/50) dynabeads and magnetic isolation of beads. Cross-links were reversed, and purified DNA was sent to the Dana Farber Center for Computation Cancer Biology (CCCB) core for library construction and sequencing. Performed by Dana Farber CCCB using NEBNext ChIP Master Mix Kit for Illumina following manufacturers instructions.
GEO Accession
GSM4547028