GSM4547729: R-1097_UWB1.289+BRCA1_ctr; Homo sapiens; RNA-Seq
RNA extraction was performed according to the miRNeasy Mini Kit (Qiagen, Hilden, Germany). On column DNA digestion was included into this protocol, in order to remove residual contaminating genomic DNA. All experiments were done according to the manufacturer's instructions. For library preparation we used the TruSeq Stranded mRNA Library Prep Kit (Illumina) according to the manufacturer's protocol, starting with 1 µg total RNA. All barcoded libraries were pooled and sequenced 2x75bp paired-end on an Illumina NextSeq500 platform to obtain a minimum of 10 Mio reads per sample.
GEO Accession
GSM4547729