16S rRNA gene V3 region of bacteria: adultfemale Wistar Hannover rats, gut microbiota
Targeted PCR (98 °C, 30 s; 98 °C, 15 s, 72 °C, 30 s for 24 cycles; 72 °C, 5 min) followed by gel purification (1.5% agarose, 100 V, 90 min)