GSM1874190: WT_FP3; Escherichia coli str. K-12 substr. MG1655; RNA-Seq
Cell pellets were cryogenically pulverized in a Spex 6870 freezer mill; 5 cycles of 1 min at 5 Hz with 1 min cooling. Cell lysates were clarified, digested with MNase, and monosomes were purified over a sucrose gradient. Ribosome footprints 15 - 45 in length and total RNA fragments 20 - 40 nt in length (for RNAseq) were PAGE purified, ligated to a linker using RNA ligase T2, converted to DNA using RT, circularized, and PCR amplified.
GEO Accession
GSM1874190