GSM3535816: PA_DMSO_1; Homo sapiens; RNA-Seq
Total RNA from HCAECs was extracted with RNeasy Mini Kit (Qiagen) and treated with on-column RNase-free DNase I following the manufacturer's protocol (Qiagen). Total RNA samples were assessed for quality with the BioAnalyzer (Agilent) RNA library preparation and sequencing were performed by the DNA sequencing core of University of Michigan. Briefly, the RNA library was prepared with TruSeq RNA Library Prep Kit v2 (Illumine, WI) and sequenced on a Hiseq 4000 platform (Illumine, WI) to generate non-stranded single end 51bp reads according to the manufacturer's protocols
GEO Accession
GSM3535816