Helicoverpa armigera, inducible Cry1Ac tolerance, egg transcriptome (Waite)
Inducible Bt tolerant H. armigera is a population of H. armigera that has been induced with a sub-lethal dose of Cry1Ac toxin for 24 generations until they are tolerant to Cry1Ac toxin. The tolerance is associated with the increase in immune activity in heamolymph. Its mode of inheritance is maternally transmitted.   Cry1Ac toxin used for the induction is prepared from cell lysate of Bt HD-73 strain that can produce Cry1Ac toxin.   mRNA extracted from 0-24 hrs-old embryos of inducible Bt tolerant Helicoverpa armigera (Waite) were sequenced using 454 sequencing.    40 mg (~ 200 embryos) were collected. mRNA was extracted using GenElute Direct mRNA Miniprep Kit (Sigma-Aldrich, USA). cDMA libraries were generated using cDNA Synthesis System Kit (Roche, USA), and cDNA Rapid Library Preparation Kit (Roche, USA).  Emulsion PCR was done using emPCR kit, Roche, USA). The library was sequenced using GS Junior sequencer.