GSM4116622: Cren7 SARC-I at pH 3 - 2; Saccharolobus solfataricus; ChIP-Seq
Crosslinked pellets were sonicated until chromatiin fragments reached 300-500bp and immunoprecipitated in triplicate using polyclonal antibody serum for Cren7 or Sso7D Samples were blunt ended, A-tailed and ligated to barcoded adapters. Libraries were used to template PCR amplificaiton for 12 cycles, and products were quantified using a bioanalyzer. ChIP and background libraries were pooled in equimolar concentrations and submitted for Illumina HiSeq3000 sequencing, yielding about 300 million 50bp reads.
GEO Accession
GSM4116622