Oil Palm Fruit and Seed transcriptome
Oil palm (Elaeis guineensis) fruit were harvested at the Centre de Recherches  Agricoles Plantes Pérennes station (Pobé, Bénin) from a dura parent of  Deli Dabou origin, within the same self-progeny of a single tree. For each stage  of development studied, three independent bunches were harvested on three  distinct individuals of the same genotype. Fifteen spikelets were then collected  in the center of each bunch and five spikelets were randomly sampled from  them. From all undamaged fruits withdrawn from each of the five spikelets,  three fruits were then randomly sampled. Then, for each stage ☓ bunch ☓  spikelet combination retained, mesocarp, endosperm, and embryo of each  fruit were separated, weighed, and flash frozen in liquid nitrogen.  Total RNA from endosperm (70, 85, 100, 120, and 160 DAP) was extracted as  described in Argout et al. (2008). Total RNA from embryos (85, 90, 100, 120,  and 160 DAP) was extracted using the lipid RNeasy Plant Kit (Qiagen). Total RNA from mesocarp (100, 120, 140, and 160 DAP) was extracted as  described previously (Morcillo et al., 2006). For fruit and seed  tissues, derived cDNAs derived from all stages of development were tagged  independently (Titanium kit, Roche Diagnostics) then mixed together for 454  pyrosequencing carried out by GATC Biotech AG (Germany) using GS FLX Titanium (Roche).