GSM1679892: Amp Rep 1; Acidovorax avenae subsp. avenae; RNA-Seq
RNA extraction and puriﬁcation were performed with RNeasy Mini Kit (Qiagen). RNA samples were treated with DNaseI and puriﬁed by phenol-chloroform-isoamyl alcohol extraction and ethanol precipitation. In order to remove rRNA and polyadenylated mRNA, ten micrograms from each total RNA sample was subsequently treated with the MICROB Express Bacterial mRNA Enrichment kit (Ambion), RiboMinus Transcriptome Isolation Kit (Bacteria) (Invitrogen) and the Sera-mag Magnetic Oligo(dT) Beads (Thermo) following the manufacturer’s instructions. Purified RNA was analyzed on a ND-8000 spectrophotometer (Nanodrop, Technologies, Wilmington, DE), agarose electrophoresis or a 2100-Bioanalyzer (Agilent Technologies, Santa Clara, CA), to determine the quantity. RNA samples were used if there was no smear on the agarose. RNA samples were also used if the 260/280 ratio was above 2.0 and the RIN (RNA integrity number) was greater than 7.0.  
GEO Accession
GSM1679892