Nlrp10 knockout mouse with Coincidental loss of DOCK8
1 ug of each DNA was fragmented using Covaris E220 (Covaris, Woburn, MA, USA) to a range of sizes centered on 300 bp. The pre-capture library was prepared using the NEBNext DNA Library Prep Master Mix Set for Illumina (New England BioLabs, Ipswich, MA, USA) including a bead-based size selection for inserts with an average size of 300 bp and 18 cycles of PCR. The resulting pre-capture library was hybridized to the Roche NimbleGen Mouse Exome capture probe set (Roche NimbleGen, Madison, WI, USA) according to the manufacturer’s instructions. The final captured libraries were amplified by 12 cycles of PCR using KAPA HiFi Hotstart Readymix (KAPA Biosystems, Wilmington, MA,USA). The resulting sequencing libraries were quantified by QPCR, pooled, and sequenced on an Illumina HiSeq 2000 (Illumina, San Diego, CA, USA).