Un-treated plants- Rep 2
Total RNAs was extracted from pools of 20 two week old non-irradiated wild type seedlings grown on 0.5x MS plates under long day conditions (16 h light/8 h dark) at 23°C. Total RNA was prepared by using TRIsure Reagent according to the manufacturer's. Polyadenylated RNAs were enriched from DNase I-digested total RNA and libraries were generated by using TruSeq® RNA Sample Preparation v2 kit (Illumina) and sequenced on an Illumina Hiseq 2000 machine.