GSM3535468: MODEK_vesicles_24hrs_1; Mus musculus; Heligmosomoides polygyrus bakeri; ncRNA-Seq
Cells were then washed twice in PBS before RNA extraction with a miRNeasy mini kit (Qiagen), according to manufacturer's instructions. The RNA Integrity Number (RIN) was tested with the Agilent RNA 6000 Pico Kit on a Agilent 2100 Bioanalyzer. Three biological replicates were included for each of the samples. Small RNA sequencing: Total RNA was treated with RNA 5' Polyphosphatase (Epicenter) following manufacturer's instructions, before library preparation. Libraries for small RNA sequencing were prepared using the CleanTag small RNA library prep kit according to manufacturer's instruction. For all samples, 1:2 dilutions of both adapters were used with 18 amplification cycles (TriLink BioTechnologies). Libraries of the length between 140-170bp were size selected and sequenced on an Illumina HiSeq high output v4 50bp SE in Edinburgh Genomics at the University of Edinburgh (Edinburgh, UK).
GEO Accession
GSM3535468