GSM4610504: TC1.P2; Homo sapiens; RNA-Seq
Subcutaneous tumors were excised and snap frozen. DNA and RNA from PDX tumours of different generations were isolated from snap frozen tumour tissue. Simultaneous isolation of DNA and RNA from the same sample was done using the AllPrep DNA/RNA mini kit (Qiagen, Hilden, Germany). The NEBNext Ultra Directional RNA Library Prep Kit (New England Biolabs (NEB), Ipswich, MA, USA), was used to process the samples. Ribosomal RNA was depleted from total RNA using the rRNA depletion kit (NEB).
GEO Accession
GSM4610504