Illumina HiSeq 2000 sequencing; Ribosome profiling and RNA-seq of in vitro differentiated human CD4+ Th1 cells.
Ribosome profiling and RNA-seq of in vitro differentiated human CD4+ Th1 cells.
Naive CD4+ T cells purified from the blood of healthy donors were polarized in vitro to TH1 in RPMI media with 10% FBS, 0.1% Penicillin/Streptomycin 0.1 % nonessential amino acids and  0,1% Sodium Pyruvate  at 37°C and 5% CO2. For Riboseq samples, RNA footprints were extracted according to Ingolia et al., 2012  DOI : 10.1038/nprot.2012.086.  Libraries for Riboseq were prepared according to Ingolia et al., 2012 DOI :10.1038/nprot.2012.086.
Experimental Factor: protocol
Ribo-seq