RNA-seq of Jatropha curcas: Geminivirus infected leaf
The total RNA was extracted from 2 different plant tissues of RJC1, and verified on an Agilent Technologies 2100 Bioanalyzer using the Agilent RNA chip manufactured. Total RNA with an RNA Integrity Number (RIN) greater than or equal to 8 was considered for library preparation. An Illumina-based library was constructed using TruSeq RNA Sample Prep Kits, as per the recommendations of the manufacturer [Illumina: Catalog # RS-930-2001]. The constructed libraries were sequenced on an Illumina HiSeq 2500 to obtain 2  100 bp PE reads per sample.