Illumina HiSeq 4000 sequencing; Thyroid deficiency before birth modifies adipose transcriptome to promote overgrowth of white adipose tissue and impair thermogenic capacity
Thyroid deficiency before birth modifies adipose transcriptome to promote overgrowth of white adipose tissue and impair thermogenic capacity
At 105-110 days of gestation (dGA: term ~145+/- 2 days) and under halothane anaesthesia (1.5% halothane in O2-N2O), twin fetuses of pregnant ewes underwent either a thyroidectomy (TX) or a sham operation in which the thyroid gland was exposed but not removed (sham). At either 129 or 143 dGA, the fetuses were delivered by Caesarean section under general anaethesia (20mg/kg maternal body weight sodium pentobarbitone I.V.). Blood samples were collected by venepuncture of the umbilical artery into EDTA-containing tubes. Each fetus was weighed and a variety of fetal organs, including perirenal adipose tissue, were collected after the administration of a lethal dose of barbiturate (200mg/kg sodium pentobarbitone I.V.).   Tissue samples were immediately frozen in liquid nitrogen and stored at -80C until analysis. RNeasy Lipid Tissue Minin Kit (Qiagen, Manchester, UK) cDNA libraries were prepared in samples with RIN>6 (Agilent bioanlyser)
Experimental Factor: injury
sham