GSM1245063: SCC-9 control; Homo sapiens; RNA-Seq
For deep sequencing, total RNA was isolated from cells using an RNeasy mini kit (Quiagen Inc. Valencia, CA, USA), including an in-column DNase treatment. RNA sequencing was performed using Illumina TruSeqRNA technology, following the manufacturer’s protocol. Briefly, mRNA is purified using poly-T oligo-attached magnetic beads and subsequently fragmented. The cleaved RNA fragments are reverse transcribed into first strand cDNA using random primers. Next, second strand cDNA is synthesized using DNA Polymerase I and RNase H. The second strand cDNA fragments are subject to an end repair, the addition of a single ‘A’ base, and ligation of the adapters. The products are then purified and enriched with PCR to create the final cDNA library.
GEO Accession
GSM1245063