GSM1874220: Sample 4 [S7]; Glycine max; miRNA-Seq
Total RNA was isolated using TRIzol reagent (Invitrogen). Small RNA libraries were constructed as described previously (Lu et al.,2007). Briefly, small RNAs in the size range of 15-30 nucleotides(nt) were purified from 200 µg of total RNA by 15% denaturing polyacrylamide gel electrophoresis (PAGE) gels and ligated to an adapter. After first-strand synthesis and 16 cycles of PCR amplification, the final bands were PAGE gel purified and submitted for sequencing on a Hi-Seq 2000 analyzer.
GEO Accession
GSM1874220