GSM4273180: 1-D0-Del; Homo sapiens; RNA-Seq
Cells were released by trypsinization, washed in phosphate-buffered saline (PBS), spun and the cell pellets frozen. DNA, RNA and protein were simultaneously extracted from pellets using the NucleoSpin TriPrep Kit (Macherey-Nagel, supplied by ThermoFisher). Deletion of the target region was confirmed by end-point PCR of DNA using primers flanking the guides (primers 5'-GAGGGGTTGAGCTGGATTCC-3' and 5'-CCAGCACCCACTCTGTAGAT-3') and by Sanger sequencing. TruSeq Stranded mRNA Sequencing NextSeq High-Output 2 x 75 bp reads (total of 7 samples)
GEO Accession
GSM4273180