RNA seq of Human FSHD Myoblasts
RNA-seq libraries were prepared from total RNA using the TruSeq RNA Sample Prep Kit (Illumina, Inc., San Diego, CA, USA) and a Sciclone NGSx Workstation (PerkinElmer, Waltham, MA, USA). Library size distributions were validated using an Agilent 2200 TapeStation (Agilent Technologies, Santa Clara, CA, USA). Additional library QC, blending of pooled indexed libraries, and cluster optimization were performed using Life Technologies' Invitrogen Qubit(r) 2.0 Fluorometer (Life Technologies-Invitrogen, Carlsbad, CA, USA). RNA-seq libraries were pooled (18-plex) and clustered across 3 flow cell lanes. Sequencing was performed using an Illumina HiSeq 2500 in rapid-output mode employing a paired-end, 50 base read length (PE50) sequencing strategy.