Knock_out_mice
The amplified fragments with adapters and Tags were quantified using Qubit fluorometer (Invitrogen) and mixed in approximately equal concentrations to ensure equal representation of each sample. A two-region 454 sequencing run was performed on a GS FLX Titanium Pico TiterPlate (70x75) using a GS FLX Titanium Sequencing Kit XLR70 according to the manufacturer's instructions (Roche). Samples barcodes: KO..01 ACGAGTGCGT KO..02 ACGCTCGACA KO..03 AGACGCACTC KO..04 ATCAGACACG KO..05 CGTCTAGTAC KO..06 TGTACTACTC KO..07 TACGAGTATG KO..08 TACTCTCGTG KO..09 TAGAGACGAG KO..10 TCGTCGCTCG KO..11 ACGCGAGTAT KO..12 ACTGTACAGT KO..13 AGCGTCGTCT KO..14 CACGCTACGT KO..15 CAGTAGACGT KO..16 TCGATCACGT KO..17 TCTAGCGACT KO..18 TCTATACTAT KO..19 TGTGAGTAGT KO..20 ACAGTATATA KO..21 ACTAGCAGTA KO..22 ACGCTCGACA KO..23 AGACGCACTC KO..24 AGCACTGTAG KO..25 ATATCGCGAG