Triphysaria versicolor, vegetative structures - leaves and stems
The Illumina mRNA Sequencing Sample Preparation Guide (1004898 Rev. D) was used with the exceptions outlined in the Illumina RNA-Seq PE Sample Prep Protocol, v3.6.  These include size selection at 300bp rather than 200bp post adapter-ligation, minimized cycles during PCR enrichment to avoid over amplification, and a second agarose gel purification of 300bp library fragments to further constrain fragment length distribution