WGS of Microbacter
For cluster generation, the library is loaded into a flow cell where fragments are captured on a lawn of surface-bound oligos complementary to the library adapters. Each fragment is then amplified into distinct, clonal clusters through bridge amplification. When cluster generation is complete, the templates are ready for sequencing. Illumina SBS technology was used on a proprietary reversible terminator-based method that detects single bases as they are incorporated into DNA template strands. As all 4 reversible,terminator-bound dNTPs are present during each sequencing cycle, natural competition minimizes incorporation bias and greatly reduces raw error rates compared to other technologies. The result is highly accurate base-by-base sequencing that virtually eliminates sequence-context-specific errors, even in repetitive sequence regions and homopolymers. This milestone is the result of relentless effort and scientific excellence led by Dr. Anuraj Nayarisseri, Principal Scientist and Director, Eminent Biosciences along with an exceptional team comprising Khushboo Sharma, Arshiya Khan, Radhika Swami, Rinku Chaudhary, Juhi Sharma, Navpreet Kaur, Jyotsana Kharate, Vasudha Rathore, Leena Prajapati and Srinivas Bandaru. Lab: https://eminentbio.com/content/membertype