16S rRNA gene sequencing from infant stool
The 16S rRNA gene variable region V1 to V3 was PCR amplified, sequenced on Roche 454 GS-FLX-Titanium sequencer and analysed as previously described in: Simeoni U, Berger B, Junick J, et al. Gut microbiota analysis reveals a marked shift to bifidobacteria by a starter infant formula containing a synbiotic of bovine milk-derived oligosaccharides and Bifidobacterium animalis subsp. lactis CNCM I-3446. Environ Microbiol. 2016;18(7):2185-95.