GSM4263877: nsun1_total_3; Caenorhabditis elegans; RNA-Seq
Nematodes were washed off the plates, lysed on ice using a pellet pestle and cleared by centrifugation. Lysates were separated on a sucrose gradient, polysomal RNA and RNA from lysate was isolated using Trizol LS using the manufacturer's protocol. RNA concentration and quality was analysed with a Bioanalyzer 2100 using the RNA 6000 Nano Kit (Agilent Technologies) before the samples were sent for next generation sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols by GATC Biotech (Konstanz, Germany).
GEO Accession
GSM4263877