p8712mo2
DNA was extracted from stool using the QIAGEN AllPrep RNA/DNA Mini kit. Whole-genome shotgun sequencing libraries were prepared according to the manufacturer's instructions using the Nextera XT DNA Library Preparation kit (Illumina) with 100-250 pg input DNA. Libraries were pooled by transferring equal volumes of each library using a Labcyte Echo 550 liquid handler. The concentrations and insert size ranges for each pooled library were checked using an Agilent Bioanalyzer DNA 1000 kit (Agilent Technologies). Libraries were subsequently sequenced on the Illumina HiSeq 2000 platform in paired-end mode (2x101bp) targeting 2Gb of sequences per sample.