GSM3140494: B31 bacterial cell; Borreliella burgdorferi; RNA-Seq
RNA was isolated from B. burgdorferi bacteria or OMVs isolated from B. burgdorferi supernatants using the miRNeasy kit (QAIGEN, Germantown MD), with additional ribosomal depletion step using the MICROBExpress™ Bacterial mRNA Enrichment Kit (Thermo Fisher Scientific). The RNA was eluted with 30 µL of nuclease-free H20 and quality was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara CA) with a RNA (Pico) chip. RNA library construction was performed using the NEBNext Ultra Library Prep Kit for Illumina. Individual library concentrations were measured using the NEBNext Library Quant Kit for Illumina and adjusted to an equal molar final pooled concentration of 2 nM and run on NextSeq sequencer (Illumina, San Diego CA).
GEO Accession
GSM3140494