Illumina HiSeq 2500 sequencing; Single-cell mapping reveals new markers and functions of lymphatic endothelial cells in lymph nodes
Single-cell mapping reveals new markers and functions of lymphatic endothelial cells in lymph nodes
LECs were isolated from inguinal LNs of C57Bl/6 wildtype mice. Cells were sorted into 384-well plates containing lysis buffer and RNA was extracted. Libraries were prepared using a miniaturised version of the Smart-seq2 protocol (Picelli et al., 2014).
Experimental Factor: single cell identifier
Inguinal_mLN_Cell_0002
Experimental Factor: cluster
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