Illumina HiSeq 2500 paired end sequencing; RNA-Seq of human micro- and macrovascular endothelial cells
RNA-Seq of human micro- and macrovascular endothelial cells
Total RNAs were isolated from 3 replicates of the CiGEnC cells and from banked primary macrovascular ECs obtained in 5 donors using an RNeasy Kit (Qiagen) including a DNase treatment step. Total RNAs were isolated using the RNeasy Kit (QIAGEN) including a DNAse treatment step. RNA quality was assessed using RNA Screen Tape 6000 Pico LabChips with the Tape Station (Agilent Technologies) and the RNA concentration was measured by spectrophometry using the Xpose (Trinean).  RNAseq libraries were prepared starting from 2 µg of total RNA using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina) as recommended by the manufacturer.
Experimental Factor: cell type
microvascular endothelial cell