GSM3140920: Fev-Cre;mTmG reporter; Mus musculus; RNA-Seq
Pancreata were isolated from mouse embryos and dissociated in TrypLE Express at 37C with pipet trituration at 5-minute intervals during incubation. E12.5 pancreata were dissociated for 10 minutes, E14.5 pancreata for 15 minutes, and E17.5 pancreata for 30 minutes. Dissociations were neutralized with FACS buffer (10% FBS + 2mM EDTA in phenol-red free HBSS). Dissociated cells were passed through a 30um cell strainer and stained with Sytox live/dead stain (Thermo Fisher), along with protocol specific stains noted in the individual sample extract protocol descriptions. Stained cells were washed twice in FACS buffer and then processed through a BD FACS Aria II. For E14.5 Fev-Cre;ROSA26mTmGpancreata, live GFP+ and Tdtomato+ cells were collected in separate tubes. cDNA libraries were prepared for sequencing using standard protocol for the 10x Single Cell instrument with v2 kit and reagents. droplet-based single cell RNA-Seq
GEO Accession
GSM3140920