GSM1606151: Cas2 bound RNAs; Pyrococcus furiosus; RIP-Seq
Protein RNA complexes were purified using antibodies specific to Cst2, Cas5t, Cas2, or Cas3-2. RNAs were then fractionated on 10% polyacrylamide gel and size selected to enrich for RNAs of 20-70 nt, RNAs were treated with thermosensitive alkaline phosphatase, and ligated to a 3' adaptor. Gel purified ligated products were then treated with T4 polynucleotide kinase and ligated to a 5' adaptor. The ligated RNAs were reverse transcribed, treated with RNaseH, and amplified by 16 cycles of PCR.
GEO Accession
GSM1606151