GSM3834527: kidney organoid, single cells, Day14rep2; Homo sapiens; RNA-Seq
Day 10, Day 12 and Day 14 kidney organoids were dissociated into single cells with accutase treatment, followed by passing through a 40 μm filter thrice to remove cell clumps. Dissociated cells were subsequently stained with trypan blue and found to have at least 80% viability. A minimum of 7000 dissociated cells were loaded per well for single cell RNA-seq library preparation with the Chromium Single Cell 3' Gene Expression Solution according to the manufacturer's recommendations. Libraries generated were sequenced on Illumina HiSeq 4000 platform.
GEO Accession
GSM3834527