GSM3834287: CIS20HL1; Mus musculus; OTHER
Cells were lysed gently and small molecular weight DNA, consisting mostly of excised 26-27 mers was separated from chromosomal high molecular weight DNA by centrifugation Briefly, the excised oligomers are immunopricipitated with anti-TFIIH (anti-XPB and anti-p62) antibodies because the excision product is released in a relatively stable complex with TFIIH, which also protects the excision product from degradation by non-specific nucleases. This is followed by extraction of the excised oligomers and ligation to adaptors and a second immunoprecipitation with anti-CPD antibodies.  Then photolyase repairs the CPD damage. This is followed by PCR amplification with index primers and NGS sequencing on HiSeq 4000 platform.
GEO Accession
GSM3834287