wild_type
he amplified fragments with adapters and Tags were quantified using Qubit fluorometer (Invitrogen) and mixed in approximately equal concentrations to ensure equal representation of each sample. A two-region 454 sequencing run was performed on a GS FLX Titanium Pico TiterPlate (70x75) using a GS FLX Titanium Sequencing Kit XLR70 according to the manufacturer's instructions (Roche). Barcodes: WT…01 AGCACTGTAG WT…02 ATATCGCGAG WT…03 CGTGTCTCTA WT…04 CTCGCGTGTC WT…05 TAGTATCAGC WT…06 TCTCTATGCG WT…07 TGATACGTCT WT…08 TACTGAGCTA WT…09 CATAGTAGTG WT…10 CGAGAGATAC WT…11 ATACGACGTA WT…12 TCACGTACTA WT…13 TCTACGTAGC WT…14 ACGACTACAG WT…15 CGTAGACTAG WT…16 ACATACGCGT WT…17 ACTACTATGT WT…18 AGACTATACT WT…19 AGTACGCTAT WT…20 ATAGAGTACT WT…21 CGACGTGACT WT…22 TACACACACT WT…23 TACACGTGAT WT…24 TACAGATCGT WT…25 TACGCTGTCT WT…26 TAGTGTAGAT WT…27 TCGCACTAGT WT…28 TGACGTATGT WT…29 ACGCGATCGA WT…30 ACGAGTGCGT WT…31 ATCAGACACG