DNA-seq of H. sapiens: pancreatic cancer patients
Genomic DNA was isolated from human peripheral blood lymphocytes by the standard Qiagen column extraction. 100 ng of DNA was fragmented to an average size 200 bp using Covaris M220 instruments with the following setting: Temperature 6C, peak incident power 75, duty factor 10, cycles per burst 200, treatment time 220s. Libraries were prepared using KAPA Library Preparation Kit for Illumina platforms. Target enrichment was performed by the SeqCap EZ Choice (Nimblegen-Roche) using a standard SeqCap protocol.