GSM2052306: E13_5_PGC_Tet1wt_6 RNA-seq; Mus musculus; RNA-Seq
FACS sorted PGCs of each embryo was diluted in Hypotonic Lysis Buffer (Amresco, Cat. M334) supplied with Recombinant RNase Inhibitor (Takara, Cat. 2313A). The Smart-seq2 RNA libraries of mouse PGCs were prepared as previously described in "Smart-seq2 for sensitive full-length transcriptome profiling in single cells" by Picelli, S., Bjorklund, A.K., Faridani, O.R., Sagasser, S., Winberg, G., and Sandberg, R. in Nature methods (2013) 10, 1096-1098.
GEO Accession
GSM2052306