GSM1603231: Tat_RNA-Seq rep2; Homo sapiens; RNA-Seq
For RNA-seq, total RNA samples were prepared from biological duplicate samples with TRIzol (Invitrogen).  rRNA-depleted total RNA was then prepared using the Ribominus isolation kit (Invitrogen) and used for library preparation using the SOLiD Total RNA-Seq kit (Applied Biosystems). Briefly, 500 ng of rRNA-depleted RNA was fragmented and adapters ligated before cDNA synthesis.  The cDNA was then size selected, amplified, purified with AmpureXP beads (Beckton Coulter) and quality checked on a Bioanalyzer (Agilent).  Samples were quantified by qPCR using SOLiD adapter primers per the manufacturer's instructions (Applied Biosystems) and the EZ-Bead system was used to amplify the libraries onto the sequencing beads for high-throughput sequencing on a SOLiD 5500xl instrument (Applied Biosystems).
GEO Accession
GSM1603231