GSM1603872: Input_RIP-seq; Mus musculus; RIP-Seq
Cell nuclei were isolated, nuclear lysate was prepared and incubated overnight with 10 µg of Zfp217 antibody (Santa Cruz Biotechnology, SC-55351x) or 10 µg of Mettl3 antibody (Bethyl, A301-567A) for 2x10E6. Afterwards, the RNA-protein complexes were immunoprecipitated with protein G magnetic beads and the RNA fraction was extracted using Trizol reagent. Library construction was prepared following the manufacturer's recommendation (TruSeq RNA Sample Preparation kit, Illumina RS-122-2001)
GEO Accession
GSM1603872