Ion Torrent PGM sequencing
A PCR amplification step with a set of six custom BKPyV specific primers covering the complete genome was used to enrich for BKPyV from the urine sample.
Libraries were prepared using the Ion Xpress Plus fragment library kit (Life Technologies). Libraries were amplified on the One Touch 2 with the Ion PGM Template OT2 400 Kit (Life Technologies), and sequenced on an Ion Torrent PGM using the 318 Chip Kitv2 and Ion PGM Hi-Q Sequencing Kit (Life Technologies).