Ammonia amended soil from 56-days aerobic dynamic: day 14
This paired-end SRA file contains the V1-V3 16S rRNA raw sequences as a multiplexed library from the 18 ammonia amended soil samples for Day 14 of the 56-days aerobic dynamic. The paired-end fastq files must be fed into the split_libraries_fastq.py script to demultiplex each Sample by its 16-bp unique barcode. The mapping file to be used is the following; where '$' must be changed to tabulation ('\t') and '@' to a new line ('\n'): SampleID$BarcodeSequence$LinkerPrimerSequence$ReversePrimer$soil_use$dinamic$Practice$Fertilizer$Day$Treatment$Pract.Day$Pract.Fertil.Day$Day.Fertilizer$Soil$OH$Description@DNA91$TAAGGCGATAGATCGC$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$amended$CT$0$14$CT0$CT.14$CT.0.14$D14.0$111$1$Soil.NH4.91@DNA92$TAAGGCGACTCTCTAT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$amended$CT$0$14$CT0$CT.14$CT.0.14$D14.0$211$2$Soil.NH4.92@DNA93$TAAGGCGATATCCTCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$amended$CT$0$14$CT0$CT.14$CT.0.14$D14.0$311$3$Soil.NH4.93@DNA94$TAAGGCGAAGAGTAGA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$amended$CT$300$14$CT300$CT.14$CT.300.14$D14.300$115$4$Soil.NH4.94@DNA95$TAAGGCGAGTAAGGAG$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$amended$CT$300$14$CT300$CT.14$CT.300.14$D14.300$215$5$Soil.NH4.95@DNA96$TAAGGCGAACTGCATA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$amended$CT$300$14$CT300$CT.14$CT.300.14$D14.300$315$6$Soil.NH4.96@DNA97$TAAGGCGAAAGGAGTA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBB$0$14$PBB0$PBB.14$PBB.0.14$D14.0$121$7$Soil.NH4.97@DNA98$TAAGGCGACTAAGCCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBB$0$14$PBB0$PBB.14$PBB.0.14$D14.0$221$8$Soil.NH4.98@DNA99$CGTACTAGTAGATCGC$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBB$0$14$PBB0$PBB.14$PBB.0.14$D14.0$321$9$Soil.NH4.99@DNA100$CGTACTAGCTCTCTAT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBB$300$14$PBB300$PBB.14$PBB.300.14$D14.300$125$10$Soil.NH4.100@DNA101$CGTACTAGTATCCTCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBB$300$14$PBB300$PBB.14$PBB.300.14$D14.300$225$11$Soil.NH4.101@DNA102$CGTACTAGAGAGTAGA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBB$300$14$PBB300$PBB.14$PBB.300.14$D14.300$325$12$Soil.NH4.102@DNA103$CGTACTAGGTAAGGAG$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBC$0$14$PBC0$PBC.14$PBC.0.14$D14.0$151$13$Soil.NH4.103@DNA104$CGTACTAGACTGCATA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBC$0$14$PBC0$PBC.14$PBC.0.14$D14.0$251$14$Soil.NH4.104@DNA105$CGTACTAGAAGGAGTA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBC$0$14$PBC0$PBC.14$PBC.0.14$D14.0$351$15$Soil.NH4.105@DNA106$CGTACTAGCTAAGCCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBC$300$14$PBC300$PBC.14$PBC.300.14$D14.300$155$16$Soil.NH4.106@DNA107$AGGCAGAATAGATCGC$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBC$300$14$PBC300$PBC.14$PBC.300.14$D14.300$255$17$Soil.NH4.107@DNA108$AGGCAGAACTCTCTAT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBC$300$14$PBC300$PBC.14$PBC.300.14$D14.300$355$18$Soil.NH4.108