RNA-Seq of vibrio alginolyticus at different cell desity
1 g total RNA was firstly treated with Antarctic phosphatase  and then with PNK enzyme. RNA Library was prepared with treated RNA according to the manufacturers instructions of TruSeq Small RNA Kit (Illumina, San Diego, USA). The libraries were size-selected for sRNA fraction (50500 nt) with 15% TBE polyacrylamide gel. Libraries were controlled for quality and quantified using the BioAnalyzer 2100 system (Agilent Technologies, Inc., USA). The libraries were sequenced for 150 pair-end cycles on Illumina HiSeq Sequencer according to the manufacturers instructions (Illumina, San Diego, USA).