Sample_10
Cells and split-pool synthesized barcoded microparticles suspended in lysis buffer are co-encapsulated into nanoliter volume droplets. Microparticles contained oligos consisting of a cell barcode (same for all oligos on a microparticle), a UMI (different for each oligo on a microparticle), a PCR handle and a polyT stretch for capture of polyA mRNA. Following encapsulation (and immediate cell lysis) mRNAs hybridize to the microparticle, the emulsion is broken, microparticles are collected and cDNA is generated through reverse transcription in bulk. For details, please see Stephenson et al., 2018 for a full description. 