RNA Seq from HEK293T B8 CKO CPSF6[551] cells
DNase-treated RNA was extracted by Sowd et al. (PNAS. 2016) from B8 CPSF6 knockout HEK293T cells (pLBNCX-CPSF6[551] complemented cells) using the Quick-RNA Miniprep kit (Zymo Research).  RNA was polyA selected, fragmented, reverse transcribed, and ligated to sequencing adapters using the TruSeq Stranded mRNA Library prep kit (Illumina). Paired end 75 bp sequencing was conducted by the Dana-Farber Molecular Biology Core Facilities using an Illumina NextSeq500.