GSM4272835: MC38-COSMC KO_3; Mus musculus; RNA-Seq
MC38 cell lines (passage 4-9, confluence 70-90%) were harvested with 1% Trypsin EDTA and washed with PBS. Total RNA was extracted with a standard TRIzol isolation protocol (Thermo Fisher Scientific, cat#15596018). Quantity and purity was tested using the Nanodrop-2000 spectrophotometer (Nanodrop Technologies). The library was synthesized using the TruSeq® Stranded mRNA Sample preparation kit (Illumina , cat# RS-122-9004), according to manufacturer's LS protocol. The product quality during library generation was analyzed on the Agilent 2100 Bioanalyzer using the DNA 7500 chip (Agilent Technologies, cat# 5067-1506).
GEO Accession
GSM4272835