GSM1924182: C3; Bos taurus; RNA-Seq
Total RNA was extracted from oviduct epithelial cells using the Trizol method as per manufacturers' instructions (Invitrogen, Carlsbad, CA, USA) and RNA clean up and on-column DNase digestion was perfored using the Qiagen Rneasy columns as per manufacturers' instructions (Qiagen, Crawley, West Sussex, UK). Standard Illumina TruSeqstranded mRNA HT library construction. One microgram of total RNAwas used as input material. mRNA was enriched using oligo-dT magnetic beads and fragmented to 100-300 bp with a single shearing and RT primer hybridization before generating cDNA. Following adapter ligation and PCR amplification (14 cycles), library validation was perfomred using the Fragment Analyzer (Advanced Analytical) with the hiSens NGS reagents followed by quantitation using the QuBit HS DNA assay (Life Technologies) and qPCR kit for Illumina (Kapa Biosystems, Inc.).
GEO Accession
GSM1924182