Phase Transition of the bacterium Mycoplasma gallisepticum Upon Invasion of a Host Cell
Genomic DNA from individual cultures was isolated as described in (Gorbachev et al., 2013), followed by RNase A (Sigma-Aldrich) treatment and ethanol precipitation. The DNA extracted (100 ng for each sample) was disrupted into 200-300bp fragments using the Covaris S220 System (Covaris, Woburn, Massachusetts, USA). Barcode shotgun libraries were prepared by the Ion Xpress™Plus Fragment Library Kit (Life Technologies). PCR emulsion was performed by the Ion PGM™Template OT2 200 Kit (Life Technologies). DNA sequencing was performed by the Ion Torrent PGM (Life Technologies) with the Ion 318 chip v2 and the Ion PGM™Sequencing 200 Kit v2 (Life Technologies).