GSM4115883: SC86; Homo sapiens; RNA-Seq
Biopsies were finely diced using a scalpel and placed in a Miltenyi C tube.  The enzymes from the Miltenyi whole skin dissociation kit, human, were used to digest cells over a period of 2 hours at 37 degrees.  Biopsies were then further mechanically separated using the h_skin_01 program on the Miltenyi OctoDissociator.  The resulting single cell suspension was filtered twice through 70 micron filters and finally resuspended in PBS containing 0.04% BSA.  Cells were loaded into the the 10X Genomics Chromium instrument according to the manufacturer's directions. Libraries were constructed according to 10X Genomics' Single Cell 3' Reagent Kits v1 and v2 User Guides single cell RNA-Seq
GEO Accession
GSM4115883