Ammonia unamended soil from 56-days aerobic dynamic: day 0
This paired-end SRA file contains the V1-V3 16S rRNA raw sequences as a multiplexed library from the 18 ammonia unamended soil samples for Day 0 of the 56-days aerobic dynamic. The paired-end fastq files must be fed into the split_libraries_fastq.py script to demultiplex each Sample by its 16-bp unique barcode. The mapping file to be used is the following; where '$' must be changed to tabulation ('\t') and '@' to a new line ('\n'): SampleID$BarcodeSequence$LinkerPrimerSequence$ReversePrimer$soil_use$dinamic$Practice$Fertilizer$Day$Treatment$Pract.Day$Pract.Fertil.Day$Day.Fertilizer$Soil$OH$Description@DNA145$TAAGGCGATAGATCGC$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$unamended$CT$0$0$CT0$CT.0$CT.0.0$D0.0$111$1$Soil.CTRL.1@DNA146$TAAGGCGACTCTCTAT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$unamended$CT$0$0$CT0$CT.0$CT.0.0$D0.0$211$2$Soil.CTRL.2@DNA147$TAAGGCGATATCCTCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$unamended$CT$0$0$CT0$CT.0$CT.0.0$D0.0$311$3$Soil.CTRL.3@DNA148$TAAGGCGAAGAGTAGA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$unamended$CT$300$0$CT300$CT.0$CT.300.0$D0.300$115$4$Soil.CTRL.4@DNA149$TAAGGCGAGTAAGGAG$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$unamended$CT$300$0$CT300$CT.0$CT.300.0$D0.300$215$5$Soil.CTRL.5@DNA150$TAAGGCGAACTGCATA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$unamended$CT$300$0$CT300$CT.0$CT.300.0$D0.300$315$6$Soil.CTRL.6@DNA151$TAAGGCGAAAGGAGTA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBB$0$0$PBB0$PBB.0$PBB.0.0$D0.0$121$7$Soil.CTRL.7@DNA152$TAAGGCGACTAAGCCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBB$0$0$PBB0$PBB.0$PBB.0.0$D0.0$221$8$Soil.CTRL.8@DNA153$CGTACTAGTAGATCGC$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBB$0$0$PBB0$PBB.0$PBB.0.0$D0.0$321$9$Soil.CTRL.9@DNA154$CGTACTAGCTCTCTAT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBB$300$0$PBB300$PBB.0$PBB.300.0$D0.300$125$10$Soil.CTRL.10@DNA155$CGTACTAGTATCCTCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBB$300$0$PBB300$PBB.0$PBB.300.0$D0.300$225$11$Soil.CTRL.11@DNA156$CGTACTAGAGAGTAGA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBB$300$0$PBB300$PBB.0$PBB.300.0$D0.300$325$12$Soil.CTRL.12@DNA157$CGTACTAGGTAAGGAG$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBC$0$0$PBC0$PBC.0$PBC.0.0$D0.0$151$13$Soil.CTRL.13@DNA158$CGTACTAGACTGCATA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBC$0$0$PBC0$PBC.0$PBC.0.0$D0.0$251$14$Soil.CTRL.14@DNA159$CGTACTAGAAGGAGTA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBC$0$0$PBC0$PBC.0$PBC.0.0$D0.0$351$15$Soil.CTRL.15@DNA160$CGTACTAGCTAAGCCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBC$300$0$PBC300$PBC.0$PBC.300.0$D0.300$155$16$Soil.CTRL.16@DNA161$AGGCAGAATAGATCGC$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBC$300$0$PBC300$PBC.0$PBC.300.0$D0.300$255$17$Soil.CTRL.17@DNA162$AGGCAGAACTCTCTAT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBC$300$0$PBC300$PBC.0$PBC.300.0$D0.300$355$18$Soil.CTRL.18