Illumina Genome Analyzer IIx paired end sequencing; RAD Sequencing of a Plutella xylostella cross
A Backcross male was crossed with an F1 female.                 22 offspring were selected for sequencing.                 12 of these offspring survived treatment with the insecticide spinosad (Bioassay 1-12, B01-B12).                 10 control offspring were untreated (Control 1-10, C01-C10).                 DNA from these 24 individuals was RAD Sequenced.                 The resulting sequences were used to construct a linkage                 map of Plutella xylostella and assess synteny with the                 silkworm Bombyx mori.
Cut genome with SbfI; ligate Illumina modified adapter containing MID (molecular identifier) and TGCA overhang; randomly shear to 300-600 bases; ligate paired end adapter; PCR amplify to select fragments containing restriction site overhangs; size select to 300-600 bases; sequence