Illumina MiSeq investigations on the changes of microbial community in the Fusarium oxysporum f.sp. cubense infected soil during and after reductive soil disinfestation
Two hundred grams soil was put into a cylindrical pot (diameter 5 cm, height 15 cm) and two treatments were performed, flooding soil with 100 ml deionized water and flooding soil incorporated with 2% (w/w) maize (Zea mays) straw (dry-heat sterilized and finely chopped, C/N ratios 41.5). Each treatment contained three pots. After anaerobic cultivated at 25 °C for 15 days, the soils (20 g in each pot) in the two treatments were collected and defined as Flooding and RSD, respectively. Then, the water flowed out through the stopcock at the bottom of the pot and 5 ml concentrated root exudates was evenly added into one pot. The soils were air-drained for 3 days, and then the pots were randomly placed into an incubator of 25 °C for 60 days with soil water contents 15%-22%, which was considered as a aerobic cultivation. The soils at the end of the aerobic cultivation in the two treatments were collected and defined as Flo-Ban and RSD-Ban, respectively. The pre-treatment soil was defined as CK. These collected soils were stored at -20 °C until the soil DNA extractions.