RNA-seq of placenta from lean and obese mothers
Total RNA was isolated from placental tissue using the Qiagen mRNAeasy kit (Qiagen, Valencia CA). RNA concentration and integrity was verified using Agilent 2100 Bioanalyzer. Total RNA was depleted of ribosomal fraction using Ribo-Gone rRNA removal kit. Libraries were then constructed using SMARTer Stranded RNA-Seq kit (Clontech, Mountain View CA).