Pseudonaja textilis NGS de novo transcriptome
RNA was enriched for mRNA. The shot gun cDNA library was done using N6 random primers. The library was normalized to enrich with underrepresented transcripts by a thermal denaturation/renaturation step and further isolation of non-renaturated sscDNA by hydroxyapatite chromatography. Those sscDNA were double-stranded by PCR and sequenced by pyrosequencing.