GSM2649659: Figure_3_Live_KO_120_1_KO_analysis; Mus musculus; RNA-Seq
RNA was extracted using TRI-reagent (Molecular Research Center), treated with RNase-free DNaseI, and purified using an RNeasy kit (Qiagen) Strand-specific libraries were generated from 400 ng total RNA using the TruSeq RNA Sample Preparation Kit v2 (Illumina), with modifications using the “dUTP” method (Levin et al., 2010). cDNA libraries were single-end sequenced with a length of 50bp on an Illumina HiSeq 2000.
GEO Accession
GSM2649659