Chlamydomonas reinhardtii Titanium Simple
Chlamydomonas reinhardtii strain cw15 petC-delta1 (wall-less strain in 137c background, mt+;ref PMID: 10521530) was cultivated in TAP medium under cool fluorescent light. Cells were subjected to various stress conditions (untreated,shift to phototrophic growth,carbon starvation,nitrogen starvation,Chloramphenicol treatment,Heat shock (40°C, 30 min)). RNA was prepared and preparations were pooled for cDNA synthesis and sequencing.
Double stranded cDNAs were constructed from purified poly(A+) mRNA cw15 strain, using random (heptamer) primers. The technique employed was that described in the CloneMiner (InvitroGen) protocol, with minor modifications.