GSM3074726: Control 3; Drosophila melanogaster; RNA-Seq
Guts were dissociated and RNA was extracted with Rneasy Mini Kit (Qiagene) Twelve  RNAseq libraries were produced using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (NEB, cat no. E7420) according to manufacture protocol and starting with 100 ng of total RNA. mRNA pull-up was performed using the Magnetic Isolation Module (NEB, cat no. E7490). libraries were mixed into a single tube at an equal molar concentration. The RNAseq data was generated on two lanes of HiSeq2500, 50SR.
GEO Accession
GSM3074726