De novo assembly of the lungfish blastema transcriptome
Total RNA extraction from blastema transcriptome was achieved using TRIzol® Reagent (Life Technologies, Cat. 15596-026) according to the manufacturer’s protocol. RNA samples were further purified using RNeasy® Mini Kit (Quiagen, Cat. 74104) and treated with DNaseI (Quiagen, Cat. 74254), according to the manufacturer’s protocol. RNA (0.5 µg) was reverse transcribed using the SuperScript III First-Strand Synthesis SuperMix ( Life Technologies, Cat. 18080-400). In order to establish a blastema transcriptome reference, a HiSeq Illumina run was obtained from blastema library with 100 bp paired-end reads