RAD-seq Scomber scombrus: Sample bob102
RAD-seq. Restriction-site-associated DNA libraries were prepared following the methods of Etter et al. (Methods Mol Biol 772, 157-178.). Briefly, starting DNA was digested with the SbfI restriction enzyme and ligated to modified Illumina P1 adapters containing 5bp unique barcodes. Pools of 33 individuals were sheared using the Covaris® M220 Focused-ultrasonicator™ Instrument (Life Technologies) and size selected to 300-500 bp by cutting agarose migrated DNA. After Illumina P2 adaptor ligation, each library was amplified using 14 PCR cycles. Each pool was paired-end sequenced (100 bp) on an Illumina HiSeq2000 and demultiplexed using the process_radtags module of Stacks (http://catchenlab.life.illinois.edu/stacks/).