RNA-Seq of cervical squamous cell carcinoma
RNA and DNA extractions were performed using the DNA/RNA All Prep kit (Qiagen). RNA extraction was followed by DNAse treatment and RNA quality was evaluated using the 2200 Tape Station (Agilent Technologies), and only samples with RNA Integrity Number (RIN) greater than or equal to 8 were included in this study. Paired-end libraries (2x100) for every RNA sample were constructed and sequenced.