GSM2212761: Wild-type Kidney cortex area_L1; Mus musculus; RNA-Seq
RNA was extracted using All prep DNA/RNA mini kit (Cat. # 80204). RNA was further treated for DNase digestion using On column Dnase digestion kit from Qiagen. MSKCC Core Facility processed the total RNA by using TruSeq RNA Sample Prep kit according to the manufacturer’s recommendation. Briefly, cDNA libraries were prepared from polyA selected RNA after end repair, A-tailing, ligation of the adaptors, and PCR enrichment. Libraries were later sequenced using Illumina HiSeq 2500 platform with 50 bp paired end reads and the total of 40 million reads per sample.
GEO Accession
GSM2212761