GSM4116586: ICC_25_Adjacent: CCA25N; Homo sapiens; RNA-Seq
Single-cell suspensions were stained for 7AAD (Invitrogen) per the manufacturer's recommendations, washed with cold PBS, and re-suspended for flow cytometric analyses. Fluorescence-activated cell sorting (FACS) -sorted viable cells (7-AAD-negative) were loaded into a well of a microfluidic chip to generate a cDNA library using a commercial 10x Genomics platform. Single-cell transcriptomic amplification and library preparation was performed using single-cell 3′ v2 chemistry (10X Genomics) according to manufacturer's instructions. The libraries were then pooled and sequenced across six lanes on an Illumina Xten or NovaSeq 6000 system.
GEO Accession
GSM4116586