Mixed DNA Sequencing On Illumina to Assess Instrument Bias
Plasmodium falciparum 3D7, Escherichia coli MG1655 and Rhodobacter sphaeroides 2.4.1 were mixed equimolar amounts to create a DNA sample which spans the GC spectrum. Paired-end Illumina shotgun libraries were created using standard library construction conditions and enriched on a Mastercycler epgradient S thermocycler (Eppendorf ) with maximum ramp speed using various PCR conditions to offset instrument sequence bias associated with sample GC content. After sequencing on a Illumina Hi-Seq with 101b PE reads, samples were analyzed for GC bias.
analysis_type
AssemblyWithReference
gssr_id
67195.0
library_type
WholeGenomeShotgun
lsid
BROAD:SEQUENCING_SAMPLE:67195.0
material_type
New Tech Library
project
G2903
work_request
23058