GSM4273224: Sample 19_S19bRNAi_T1; Drosophila melanogaster; RNA-Seq
RNA was isolated using TRIzol (Invitrogen, 15596026) or phenol-chloroform treatment, treated with DNase (TURBO DNA-free Kit, Life Technologies, AM1907), and then run on a 1% agarose gel to check integrity of the RNA. To generate mRNA-enriched libraries total RNA was treated with poly(A)tail selection beads and then following the manufacturer's instructions of the NEXTflex Rapid Directional RNAseq Kit (Bioo Scientific Corp, NOVA-5138-08), except RNA was fragmented for 13 minutes. Following the manufacturer's instructions of the NEXTflex Rapid Directional RNAseq Kit (Bioo Scientific Corp, NOVA-5138-08), except RNA was fragmented for 13 minutes
GEO Accession
GSM4273224