Transcriptional programs in mouse kidney development using deep RNA-sequencing
Total RNA (20ug) from Embryonic kidney (15.5dpc) of CD1 mice (oligoDT poly(A) ) was used to generate fragment cDNA libraries. Libraries were generated as specified in the Whole Transcriptome Analysis Kit (Ambion) protocol for mRNA and Short RNA Expression (SREK) sequencing for small RNAs.