Complete genome sequencing of S. paucimobilis AIMST S2
Genomic DNA extraction was performed according to the GeneJet Genomic DNA purification kits protocol. The concentration and quality of extracted DNA was determined using Nanodrop  Lite spectrophotometer (Thermo Scientific, Wilmington, DE, USA), Qubit dsDNA BR assay (Thermo Fisher Scientific, Wilmington, DE, USA) and agarose gel electrophoresis. Purified genomic DNA was sequenced on MK1B (MIN-101B) MinION platform with a FLO-MIN 106 R9 (SpotON) flow cell using MinKNOW software version 2.0 (Oxford Nanopore Technologies) according to manufacturers instructions.