NextSeq 500 paired end sequencing
DNA extracted from cultured cells for illumina sequencing
Genomic DNA was purified from a cell pellet using NucleoSpin� Tissue kit (MACHEREY-NAGEL, 740952). A paired-end library was constructed with an insert size of 452 bp in average using NEXTflex Rapid DNA Sequencing Kit (BiooScientific). Short reads of 150 bp were generated on NextSeq 500 platform using a high output flowcell. The sequencing run have been demultiplexed with one mismatch allowed in the index. The demultiplexed reads were trimmed using cutadapt (version 1.9.1 with options: -q 30 -m 115). The resultant reads were corrected using lighter (version 1.1.1).