RNA sequencing of legs from C. lapponica female adults that feed on birch.
Around 2.5 g of total RNA was then used for library preparation using TruSeq  RNA Sample Prep Kit v2 according to manufacturer s description. The library was sequenced using a HiSeq2000 (Illumina) in 50bp single read mode using sequencing chemistry TruSeq SBS Kit v3 (Illumina). The sequencing approach resulted in around 50 mio. reads per sample. Reads were extracted in FastQ format using CASAVA v1.8.2 (Illumina) and used for further analysis.