GSM2991430: D4-Sham-H3K27ac; Homo sapiens; ChIP-Seq
ChIP extracts were sonicated nine times for 5 min each round (30 sec of sonication with intermediate incubation of 30 sec per round) using a Bioruptor (Diagenode). All ChIPs were performed using 500 μg of extract and 2 μg of antibody per sample. ChIP DNA was also used to make sequencing libraries using NEBNext Ultra DNA library preparation kit for Illumina (New England Biolabs).
GEO Accession
GSM2991430