GSM3141674: Tim-4–CD4– small intestine macrophages  - replicate 3 from 3 pooled mice; Mus musculus; RNA-Seq
Cells were isolated by fluorescence-activated cell sorting (FACS) from the small intestine or peripheral blood of 3-4 pooled adult wild type C57/BL6 mice. Cells were sorted directly into RLT buffer (Qiagen) and stored on dry ice before storage at -80oC for subsequent RNA extraction. RNA was extracted from 120,000 – 200,000 cells using an RNeasy micro kit (Qiagen) following manufacturer's instructions. Strand-specific RNA-seq libraries were prepared using the Illumina workflow with the TruSeq® Stranded mRNA Sample Preparation Kit.
GEO Accession
GSM3141674