RNAseq of S. cerevisiae GHP4 no inhibitors
The  transcriptomes  were  sequenced  using  Illumina  miSeq  paired  end  sequencing and standard methods (Illumina Inc, San Diego, CA). Total  RNA  was  prepared  from  each  sample  using  Zymo  YeaStar  kit  (Zymo  Research  Corp,  Irvine,  CA)  following  the  manufacturers  instructions.  One library for each strain and growth condition was pre-pared, including parent XR122N in YPD. Briefly, mRNAs were  isolated,  cDNA  synthesized,  and  the  libraries  finalized  according  to  manufacturers  instructions  (Illumina  Inc, San Diego, CA). All sequencing was performed at the University of Georgias Georgia Genomics Facility.