Metagenomic shotgun sequencing of stool: Homo sapiens
PSP Spin Stool DNA Plus Kits were used for DNA purification. NanoDrop 2000 spectrophotometer, Qubit fluorimeter (Thermo Fisher Scientific Inc., MA, USA) and agarose gel electrophoresis were used to analyze the concentration, integrity, and purity of DNA samples. All DNA samples were sequenced on an Illumina platform with paired-end reads of 150 bp.