DNA-seq of Paracoccus kondratievae BJQ0001
Genomic DNA was sheared into smaller fragments with a desired size by Covaris S/E210 (Covaris, USA) firstly. Then the overhangs resulting from fragmentation are converted into blunt ends by using T4 DNA polymerase, Klenow Fragment and T4 polynucleotide kinase. After adding an A base to the 3' end of the blunt phosphorylated DNA fragments, adapters are ligated to the ends of the DNA fragments. The desired fragments are purified though gel-electrophoresis, then selectively enriched and amplified by polymerase chain reaction (PCR). The index tag could be introduced into the adapter at the PCR stage as appropriate and a library quality test was carried out thereafter. The library products are finally ready for sequencing.