GSM1681732: Sample B; Ovis aries; RNA-Seq
Rumen papillae, recovered at slaughter, were snap frozen in liquid nitrogen the stored at -80 °C. Total RNA was isolated ~10 mg of tissue using an RNeasy mini kit (Qiagen, Hilden, Germany) and an on-column DNase digestion (Qiagen, Hilden, Germany) according to the manufacturer's instructions. . RNA quality was assessed using an Agilent® RNA 6000 Nano Assay on an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), and total RNA was quantified using the NanoDrop® ND-1000 spectrophotometer (NanoDrop Technologies Inc., Wilmington, DE, USA). Illumina cDNA libraries were prepared following the TruSeq™ Stranded mRNA sample preparation guidelines. Libraries were visualised using an Agilent® DNA High Sensitivity assay on an Agilent 2100 Bioanalyzer, and quantified using the Qubit® dsDNA HS assay (Invitrogen, Life Technologies) according to the manufacturer's instructions. Qubit concentrations were used to pool the two indexed cDNA libraries containing the specific Illumina TruSeq adapters. The denatured cDNA templates and PhiX control (Illumina, CA, USA) were pooled together and sequenced using an Illumina MiSeq Nano Kit v2 (300 cycles), resulting in 150 bp paired-end reads.
GEO Accession
GSM1681732