GSM1246825: 16L RNA-seq; Homo sapiens; RNA-Seq
Total RNA from human skin fibroblasts was prepared using PureLink RNA Mini kit (Ambion, Cat#12183018A). The cells were lysed by addition of lysis buffer, the lysate was then homogenized using QIAshredder (QIAGEN, cat# 79654) by centrifugation at 14,000g for 2 min, and then processed following the manufacturer's instructions. The isolated total RNA was aliquoted, and the quality of the prepared RNA was evaluated using Bioanalyzer. The RNA samples were used for RNA-seq with a range of RIN values between 8.50 and 10. A cDNA library for deep sequencing was prepared using oligo d(T) primer. The Illumina protocol (kit) was followed for later steps. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM1246825