GSM1873240: Ph_WT expressing S2 cells, rep.1; Drosophila melanogaster; RNA-Seq
Total RNA was isolated using Qiagen RNA easy kit, ribosomal RNA was depleted by using ribozero gold kit and cDNA was prepared (and fragmented) by using Trueseq kit Sequencing libraries were generated as described previously (Bowman et al., 2013, BMC Genomics, 14: 466). Briefly, ends of the immunoprecipitated DNA were repaired, followed by A-tailing, ligating to universal adapters, and amplification for 10 cycles with indexed primers. Excess adapters were removed by purification with Agencourt AMPureXP beads (Beckman-Coulter). Fragment size was checked by Bioanalyzer using a high sensitivity DNA chip, and an average size distribution of 300-400 bp was observed.
GEO Accession
GSM1873240