GSM3074696: Coemansia spiralis, WGBS; Coemansia spiralis; Bisulfite-Seq
Candida albicans, Candida auris, and Clavispora lusitianiae. MasturePure yeast DNA purification kit (Epicentre) following the manufacturer's instructions. Microbotryum lychnidis. Qiagen Kit 10243 (Courtaboeuf, France) following the manufacturer's instructions and using a Carver hydraulic press (reference 3968, Wabash, IN) for breaking cell walls. Lobosporangium transversale, Syncephalis fuscata, Spinellus fusiger, Parasitella parasitica, Kirkomyces cordense, Radiomyces spectabilis, and Sporobolomyces roseus. CTAB/Chloroform protocol (http://1000.fungalgenomes.org/home/protocols/high-quality-genomic-dna-extraction/). Aureobasidium pullulans, Coemansia reversa, Coemansia spiralis, Coprinopsis cinerea, Heterobasidion irregulare, Mixia osumundae, Phanerochaete chrysosporium, Pleurotus ostreatus, Pseudogymnoascus destructans, Tilletiopsis washingtonensis, and Wolfiporia cocos. A modified CTAB/Chloroform protocol (James et al. 2008; doi: 10.1111/j.1558-5646.2008.00462.x). Pholiota alnicola. EZNA Fungal DNA Mini Kit (Omega Bio-tek, Norcross, GA) following the manufacturer's instructions. MethylC-seq libraries were prepared using the protocol described in Urich et al. (2015; doi:10.1038/nprot.2014.114). Lambda phage DNA was used as a control for the sodium bisulfite non-conversion rate.
GEO Accession
GSM3074696