Illumina MiSeq paired end sequencing; RNA-seq of 12-weeks-old male murine heart ventricles.
RNA-seq of 12-weeks-old male murine heart ventricles.
Ventricles were freshly dissected in cold PBS and snap frozen on liquid nitrogen. Heart tissue was homogenized in TRIZOL (Ambion, 15596026) using a Tissue Lyser LT (Qiagen). RNA was purified using the columns from RNeasy Mini Kit (Qiagen, 74104) according to the manufacturer instructions. Sequencing libraries were prepared using TrueSeq Stranded RNA LT Kit Ribo-Zero Gold (Illumina, 15032619).
Experimental Factor: genotype
Ephb4 Flox/Flox; Cdh5 CreERT2/+