GSM1603948: coi1-16 ein2 sid2 ctrl rep3; Arabidopsis thaliana; RNA-Seq
Ten to 15 rosettes per genotype (Col-0 set1, coi1-16 ein2 sid2, Col-0 set2, tga2 tga5 tga6 ) from control and ozone treatment were pooled and frozen into liquid nitrogen. Three biological replicates of RNA were isolated with GeneJet Plant RNA purification Mini Kit (Fermentas, now part of Thermo Scientific). Quality and quantity of RNA was assessed using Agilent 2100 Bioanalyzer and NanoDrop. Libraries were prepared from 100 ng of total RNA with Illumina TruSeq RNA Sample Prep Kit v2 according to the kit manual.
GEO Accession
GSM1603948