NextSeq 550 sequencing; RNAseq analysis of colon and ileum tissue from naive wild type, MR1-/- and IL-17A-/- mice
RNAseq analysis of colon and ileum tissue from naive wild type, MR1-/- and IL-17A-/- mice
The mice were germline knockouts for MR1 and IL-17A expression. MR1–/– mice on a C57BL/6-background (Treiner E etal., Nature, 2003) were provided by Prof O Lantz, Institute Curie, Paris, France. IL-17A–/– mice on a C57BL/6-background were provided by Dr Iwakura, University of Tokyo, Japan. All mice were bred and housed in the QIMR Berghofer Animal Facility, Brisbane, Australia. Tissue from proximal colon and distal ileum was collected from the same mice at 8-12 weeks of age.The mice were germline knockouts for MR1 and IL-17A expression. MR1–/– mice on a C57BL/6-background (Treiner E etal., Nature, 2003) were provided by Prof O Lantz, Institute Curie, Paris, France. IL-17A–/– mice on a C57BL/6-background were provided by Dr Iwakura, University of Tokyo, Japan. All mice were bred and housed in the QIMR Berghofer Animal Facility, Brisbane, Australia.  Total RNA was isolated from proximal colon and distal ileum tissue using Precellys Lysing and Qiagen RNeasy Plus Mini kits as per manufacturer's instructions.  RNA libraries were prepared using the NEBnext Ultra RNA Library Prep Kit for Illumina (New England Biolabs), assessed for size and quantified using the 2100 Bioanalyzer (Agilent Technologies) and Qubit fluorometer (Thermofisher Scientific), respectively.
Experimental Factor: genotype
MR1-/-
Experimental Factor: organism part
ileum