Illumina MiSeq paired end sequencing; Stabilized biological production by product addiction
Stabilized biological production by product addiction
A serial-passage evolution experiment was performed with the studied strains. Upon each new serial passage (seed), a time-series sample (2 mL culture) was collected and stored at -20 deg. C. according to detailed specifications in manuscript. Seed number thereby refers to the number of passages that the population has been through, each passage was taken after a 16 hour time point. Growth in M9 medium according to manuscript specifications. As described in the manuscript, eight parallel lineages (c1-4 and c6-9) of E coli equipped with a mevalonic acid production pathway were cultured by serial passing. Each new serial passage/seed, (2 % volume) was conducted following 16 hours of cultivation to avoid stationary-phase culture.  Nucleic acids extracted by standard plasmid extraction kit (Macharey Nagel) Nextera XT v2 with two extra limited PCR cycles
Experimental Factor: individual
c7
Experimental Factor: passage
13
Experimental Factor: genotype
e3.9 (atoB, mvaS, mvaE, araC, pfolP::pBAD)