16SrRNA of Buffalo: Fresh_07
DNA was extracted from the Bushmeat samples and the V4 hyper-variable region of the 16S rRNA gene was amplified from the DNA extracts during the first PCR step using universal 515F forward primer (5 AATGATACGGCGACCACCGAGATCTACAC TATGGTAATT GT GTGCCAGCMGCCGCGGTAA 3) and unique 806R reverse primers for each sample.