GSM3141833: SCC25 PBS 5d [HFNYHBCXY_2_GCCAAT]; Homo sapiens; RNA-Seq
Total RNA was isolated from at least 1000 cells using the TRIzol Reagent (Invitrogen) according to manufacturer's instructions. The RNA concentration was determined by the spectrophotometer Nanodrop (Thermo Fisher Scientific, Waltham, MA) and quality was assessed using the 2100 Bioanalyzer (Agilent, Santa Clara, CA) system. An RNA Integrity Number (RIN) of 7.0 was considered as the minimum to be used in the subsequent steps for RNA-seq. Library preparation was performed using the TrueSeq Stranded Total RNAseq Poly A1 Gold Kit (Illumina, San Diego, CA), according to manufacturer's recommendations, followed by mRNA enrichment using poly(A) enrichment for ribosomal RNA (rRNA) removal.
GEO Accession
GSM3141833