GSM2649439: patient_19_High_Pain_sRNA; Homo sapiens; RNA-Seq
Synovial tissues were collected from knees of OA patientsat the time of the join-replacement surgery and total RNA was extracted according to the modified Chomczynski and Sacchi protocol,by using TRIzol Reagent (ThermoFisher) For mRNA sequencing, the Illumina TruSeq® Stranded total RNA protocol with the TruSeq Stranded total RNA Sample Preparation Kit. For small RNA sequencing, libraries were produced by NEBNext® Small RNA Library Prep Set for Illumina® (NEB #E7330S/L)
GEO Accession
GSM2649439