GSM2124177: 3.Ctrl; Drosophila melanogaster; RNA-Seq
For each sample, 5 male flies were lysed in 200 ul TRIzol reagent and total RNA was extracted according to the manufacturer's instructions, followed by an extra round of ethanol precipitation. Libraries were prepared using Illumina TruSeq Stranded mRNA v2, with the following changes from the manufacturer’s protocol: starting samples were 250ng total RNA; fragmentation was performed for 10 minutes at 94°; no spike-ins were used; and 14 cycles of PCR were used to enrich libraries. Libraries were sequenced on an Illumina NextSeq 500 using v1 chemistry.
GEO Accession
GSM2124177