GSM3140527: HAM Donor 9 24h Mtb; Homo sapiens; RNA-Seq
Cells were lysed in Trizol. Ethanol was added to the Trizol lysate and the mix was applied to Directzol columns (Zymo Research). Columns were washed and RNA eluted per manufacturer instructions. The AmpliSeq human transcriptome gene expression kit was used to prepare libraries covering 20,803 human transcripts, including coding and non-coding RNA. cDNA was synthesized from 10 ng of total RNA. Targeted amplification, digestion, adapter ligation and cleanup was per protocol. Libraries were quantified with qPCR.
GEO Accession
GSM3140527