GSM4116710: HA_ChIPSeq 1; Mus musculus; ChIP-Seq
The anterior and posterior palate tissues were dissected out under a fluorescence dissecting scope from Shox2HA-DsRed embryos. Pooled palatal tissues were subjected to ChIP using anti-HA (Abcam, ab9110) or anti-H3K27ac antibodies (Abcam, ab4729) following the manufacturer's protocol. Three independent ChIPs were conducted for library generation for each group. The libraries were then sequenced on the Illumina HiSeq 2000 platform using 50-bp single-end configuration. HA-enriched or H3K27ac-enriched genomic regions of the Shox2-expressing anterior palate and the posterior palate were analyzed and intersected comprehensively. ChIP-Seq libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM4116710