Aedes albopictus female reproductive organs transcriptome
cDNA library sequencing was conducted at the Genomics Core Facility of the Life Sciences Core Laboratories Center at Cornell University.  The cDNA was fragmented and converted to sequencing libraries using the Roche/454 Rapid Library preparation kits and protocols (454 Life Sciences, Branford, CT). Each sequencing library was created independently and tagged with a multiplex identifier (MID) using the Roche/454 Rapid Library MID Adaptors kit (454 Life Sciences, Branford, CT). The final libraries were quantified using fluorometry and average fragment sizes were determined using an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA). The samples were pooled in equimolar ratios, diluted, and subjected to emulsion PCR according to the manufacturer's protocols (454 Life Sciences, Branford, CT#. The pooled libraries were sequenced on a full PicoTiterPlate #PTP) on a GS-FLX sequencer using the Titanium chemistry (454 Life Sciences, Branford, CT). Image processing, basecalling, separation by MID, and adaptor trimming were performed using the bundled data collection and analysis software.