leaf transcriptomes of Zea mays
Maize inbred line A188 was grown in a greenhouse under controlled conditions (20-28℃, day length 16 h). M. separata were obtained from the Institute of Zoology, Chinese Academy of Sciences. OS were collected 3 days after M. separata were reared on A188 leaves. W+W and W+OS treatment were done as described previously by gently rubbing 20 µl of water or M. separata OS to wounds generated by rolling a pattern wheel along the midvein generating three rolls of wounds on each side on the 3rd full expanded leaves of V3 stage plants. (Wu et al., 2007). Samples were harvested at the indicated times after treatments and immediately frozen in liquid nitrogen, and were stored at -80 oC until use. Libraries from the maize leaf total RNA (3µg) were prepared using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations and sequenced on a Illumina HiSequation 2000 system with TruSeq PE Cluster Kit v3-cBot-HS (Illumia).