Whole transcriptome sequencing of Pistacia vera
High-quality total RNA was extracted from leaf, stem and root of pistachio plants and mixed an equal amount to make a single pool. Following mRNA enrichment using magnetic oligo (dT) beads, library preparation from the pooled RNA sample was performed as outlined in Illumina’s TruSeq Stranded mRNA Sample Prep Kit. The cDNA library was sequenced on the one lane of Illumina Hiseq 2000 platform as 2 × 100 run.