GSM2213998: IPF SL32679; Homo sapiens; RNA-Seq
Five 10-µm slices of the whole lung tissue were cut from FFPE block, excess paraffin was trimmed and slices were treated twice with 1ml xylene for 30 min at 62°C, then washed twice with 100% ethanol. Total RNA was isolated by using MasterPure kit (Epicentre Biotechnologies). Ribosomal RNA was removed by using the RiboZero rRNA removal kit (Epicentre) NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (New England Biolabs) following manufacturer’s protocol from 1ug of total RNA
GEO Accession
GSM2213998