GSM1606377: Mutant; Solanum lycopersicum; ncRNA-Seq
Total RNA was extracted using RNeasy plant miniRNA extraction kit (Qiagen) from 7B-1 and WT anthers of different sizes and pooled separately in equimolar ratio Two small RNA libraries were constructed using the TruSeq Small RNA Sample Preparation Kit from Illumina (Illumina, San Diego, CA, USA). In brief, small RNA fractions of 18–28 nt were isolated from 15% denaturing polyacrylamide gels. The fractionated small RNAs were ligated to 3’ TruSeq adaptors and then converted to DNA by RT-PCR following the kit protocol.
GEO Accession
GSM1606377