GSM1873338: circ-RNAseq: si-Ctrl rep2; Homo sapiens; ncRNA-Seq
Total RNA was extracted with Trizol reagent (Life Technology). The Ribominus kit (Life Technology) was employed to remove rRNA. RNase R treatment was performed with 20U RNase R (Epicenter) per μg RNA at 37°C for 15 min. RNAs with or without RNase R treatment were purified and subjected to library preparation and next generation sequencing. RNA-seq libraries were constructed with TruSeq Stranded mRNA Library Prep Kit from Illumina according to the manufacturer’s manual.
GEO Accession
GSM1873338