GSM2054559: Single neuron 16; Homo sapiens; RNA-Seq
After recording, the wider tip of another pipette filled with no more than 0.5 µL sterilized cell harvest solution containing (in mmol/L) 144 K-gluconate, 3 MgCl2, 0.5 EGTA and 10 HEPES (pH 7.20 and 295 mOsm/kg) was gently attached to the recorded cell, then a light suction (negative pressure) was applied, through a glass syringe (Thomas Scientific) connected to the pipette. The suction lasted until the entire cell entered the tip of the pipette. The moment the complete cell had been visually located inside the tip, the pipette was quickly removed from the bath. Content of the harvest pipette was expelled into a 0.2 mL PCR tube containing 4.5-µL pre-prepared lysis buffer. Preparation of single-cell cDNAs was performed based on previously published protocols with modifications at several steps.
GEO Accession
GSM2054559