RNA-Seq of A.thaliana: whole seedlings
Arabidopsis seeds were germinated and grown on 1/2 MS for 5 days, then seedlings were transferred to new 1/2 MS plates containing DSF or DMSO (control) for an additional 3 days. After 3 days, seedlings were splitted into 2 separate sets of samples to be subjected to a quick (2h) treatment with water or 150nM flg22 peptide at room temperature. Seedlings were then rinsed, blotted dry and snap-frozen in liquid nitrogen before RNA extraction. Quality control was performed using Bioanalyzer kit. RNA Sequencing was done with Illumina HiSeq 2500 system.