GSM4116050: Mono; Homo sapiens; RNA-Seq
RNA was extracted using total RNA purification kit (NORGEN), RNA from Candida albicans treated and untreated monocytes derived from 3 different donors were equaly pooled (total 3 μg) and sequenced by LCSciences LCSciences: RNA was subjected to isolate Poly (A) mRNA with poly-T oligoattached magnetic beads (Invitrogen). Following purification, the poly(A)- or poly(A)+ RNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 190-200 bp. And then we performed the paired-end sequencing on an Illumina Hiseq 4000 at the (lc-bio, China) following the vendor's recommended protocol.
GEO Accession
GSM4116050