pooled foot tissue from Margarella antarctica
M. antarctica were collected by SCUBA divers from depths of 10-15 m from South Cove, near Rothera research station, Adelaide Island, Antarctic Peninsula (67o 34? 07? S, 68o 07? 30? W) in the austral summer of 2012. 20 animals were dissected and the foot tissue flash frozen in liquid nitrogen and stored at -80°C until further analysis. Total RNA was extracted from all 20 individuals TRIsure Reagent (Bioline) according to manufacturer’s instructions. RNA quality and concentration were determined using a NanoDrop ND-1000 Spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA), respectively. The RNAs from all the individuals were pooled for RNA-Seq. The production of the 454 library and the transcriptome sequencing was performed at the University of Cambridge Sequencing Service in the Biochemistry Department (Cambridge, UK).