Characterization of Setaria equina HSP70 DNA
Total DNA was isolated from S. equina adult worms using commercial DNA extraction kit (Invitrogen, California, USA). The complete genomic sequence for S. equina was obtained by illumina technology of next generation sequencing which was performed in GATC biotech (Konstanz, Germany). The genomic sequence was published in National Center for Biotechnology Information under assembly number GCA_003012265.1. Based on DNA sequence homology studies with other filarial species, complete sequence of rSeqHSP70 was