GSM2218896: NO; Homo sapiens; ncRNA-Seq
The tissue samples from two ovarian cancer patients and one healthy donor was surgically resected and immediately placed into RNAlater solution (Invitrogen) at room temperature. These samples were collected from the neighbouring hospitals in the state. The median age of the donors was 60 years. All patients provided written informed consent. The IEC approval has been taken to carry out this work. The resected ovarian cancer tissues were characterized as endometrioid and serous type from the tissue biopsy. The samples were stored at -80°C until next-generation sequencing and other experiments were performed. Total RNA was extracted from all three tissue samples (ENOCa, SOCa, and normal ovary) with the use of RNeasy Mini kit (Qiagen, USA) according to the manufacturer’s instructions. The purity of isolated RNA was evaluated using NanoDrop spectrophotometer (Thermo Scientific) by measuring absorbance at 260 nm and 280 nm. An aliquot of each the samples of ENOCa, SOCa, and normal ovary was also run on an Agilent RNA Bioanalyzer chip to check for RNA integrity number (RIN) and was found to be 7.7, 7 and 6.3 respectively. The RNA libraries were constructed for sequencing following Illumina TruSeq Small RNA library protocol outlined in “TruSeq Small RNA Sample Preparation Guide” (Part # 15004197; Rev. F; February 2014) and using TruSeq Small RNA sample preparation kit (Illumina, # RS-200-0012). The library was size selected in the range of 145 – 165 bp.
GEO Accession
GSM2218896