GSM1244705: challenged-severe pathology (SV); Gallus gallus; RNA-Seq
Total RNA was isolated from each spleen using Trizol (Invitrogen, CA, USA) according to manufacturer’s instructions, and thereafter, three mixed RNA samples for NC, MD and SV were prepared by quantification and pooling. Twenty ug of total RNA were purified by denaturing 15% PAGE to enrich for 16-32 nt small RNAs, and then ligated with proprietary adapters. Subsequently, cDNA was synthesized from small RNAs by reverse transcription and amplified with 15 PCR cycles to produce libraries. Amplified products were validated and quantified using an Agilent 2100 Bioanalyzer and the DNA 1000 Nano Chip Kit (Agilent, Technologies, Santa Clara, CA, USA).Deep sequencing was performed on the Genome Analyzer IIx.
GEO Accession
GSM1244705