GSM1245899: C5 control; Homo sapiens; RNA-Seq
A published iCLIP protocol was followed (Konig et al., 2010, 2011) with the following exceptions: phosphatase treatment was performed using shrimp alkaline phosphatase (SAP), the irrelevant immunoprecipitation was performed with a rabbit affinity purified antibody to zebra finch FMRP, described in (Winograd et al., 2008). During the iCLIP protocol, once the RNA was isolated, primers containing unique sequences as bar codes for each sample were utilized for the priming of the reverse transcription reaction. These primers also contained sequences complementary to Solexa P5 and P3 primers that were subsequently utilized for second strand synthesis and eventual PCR amplification. The tagged and amplified cDNA was then put on the HiSeq2000 for sequencing.
GEO Accession
GSM1245899