GSM2212602: miR17_92_tKO3_25.5hr; Mus musculus; RNA-Seq
Purified ribosome footprints were extracted using miRNeasy Kit from Qiagen (cat. no. 217004) RNA libraries were prepared for sequencing using ribosome profiling libary prep protocol (Ingolia et al., Nature Protocol 2012). Indexed footprint library was generated with 4 or 6 cycles of PCR using index library primers, and avoided broad, slower migrating smear bands.
GEO Accession
GSM2212602