Microbial diversity of a limestone cave of Andamans
Genomic DNA was isolated from 5 g of the cave wall scrapings using Nucleospin Soil Kit (Macherey-Nagel, Germany) following the manufacturers protocol. 2 l of isolated DNA sample was resolved on 0.8% Agarose gel at 120 V for 60 min and 1 l of DNA sample was loaded in NanoDrop for determining A260/A280 ratio.