GSM1681427: Input (PU.1) - baseline Macrophage; Homo sapiens; ChIP-Seq
Chromatin from 0.5x10E6 macrophages was cross-linked for ChIP reactions with 1 % formaldehyde. Nuclei were lysed and chromatin was sheared with the Covaris S220 ultrasound system. For ChIP antibody reactions a PU.1 polyclonal rabbit antibody was used (SantaCruz, sc-352). Washing and DNA purifications as well as size selections were performed with AMPure XP SPRI beads (Beckman Coulter). Published library construction protocol was adapted (Blecher-Gonen et al., Nature Protocols, 2013) and performed with 0.5 ng ChIP DNA. Barcodes from the NEXTflex adapter oligonucleotides kit (Bioo Scientific) were ligated to the ChIP-seq DNA fragments. Final DNA purification with additional size selection was performed with SPRI beads. ChIP-seq libraries were sequenced on the HiScanSQ/Hiseq 1000 sequencer (Illumina)
GEO Accession
GSM1681427