WGS PacBio RS II of V. vinifera Chardonnay clone I10V1
Nuclear DNA was isolated from early season, disease free, field grown Chardonnay leaves taken from plants at a nursery vineyard. DNA was extracted from nuclear-enriched material using a CTAB/Chloroform method. DNA from clone I10V1 was enriched using a 1:0.45 Ampure cleanup prior to being used to build 15-50 kb SMRT Bell libraries with Blue Pippin size selection following library preparation.