Replicate sequencing of bacterial monoisolates
The replicate study consists of 3 isolates for each species, obtained from Westchester Medical Center. For each isolate that is sequenced, two additional sequencing replicates, two additional library replicates, and two additional colonies from the same plate are analyzed. Two additional culture replicates (from the same colony) are sequenced one and two days after the original isolate, yielding a total of 9 replicates for each isolate. The libraries are prepared using Nextera XT DNA library preparation kits and sequenced using Illumina 2 x 150 bp reads.