GSM4273875: Oil_3; Rattus norvegicus; ncRNA-Seq
The serum was centrifuged at 16,500 g for 20 min at 4°C, and the supernatant was obtained (pretreated serum). The pretreated serum (2.7 mL) was centrifuged at 208,000 g for 70 min at 4°C and the supernatant was discarded. The exosome pellet was resuspended in PBS and centrifuged again at 208,000 g for 70 min at 4°C. The supernatant was discarded, and then QIAzol Lysis Reagent were added to the exosome pellet for RNA extraction. The total RNA was extracted using miRNeasy Serum/Plasma Kit according to the manufacturer's protocol. cDNA libraries for small-RNA sequencing were synthesized with a NEBNext Multiplex Small RNA Library Prep Set for illumina and quantified with a Qubit 2.0 fluorometer (Life Technologies, Carlsbad, CA) using Qubit dsDNA HS Assay Kit (Life Technologies) according to the manufacturer's protocol. Small-RNA sequencing was performed with an Illumina MiSeq System using MiSeq Reagent Kit v3. For the improved cluster formation, denatured PhiX Control v3 was added to the cDNA libraries.
GEO Accession
GSM4273875