GSM4273025: young-homo2_RNA-seq; Mus musculus; RNA-Seq
Harvested spleens were mechanically dissociated to form a single cell suspension. Erythrocyte lysis was carried out using ACK buffer and B cells were immunomagnetically selected from the resulting cell suspension using the MACS B cell Isolation Kit. Cells were washed in cold PBS and total RNA was extracted using Qiagen RNAeasy kit. Libraries were prepared using the TruSeq RNA Sample Prep Kit v2 (Illumina).
GEO Accession
GSM4273025