16S rRNA sequence: Antarctic Marine Sediment
PCR reaction was carried out with a thermal cycler (Thermo Scientific, USA), using 25 ?L of KAPPA HiFi HotStart Ready Mix (KAPPA Biosystems) polymerase, 5 ng of DNA and 0.2 ?M of each primer, under the following conditions: 95 C for 3 min, 30 cycles of 95 C for 30 s, 55 C or 67 C for 30 s (for Bacteria and Archaea, respectively), 72  C for 30 s and a final extension of 72 C for 5 min.