16S rDNA amplicon seq of bacteria: rhizospheric soil
Total metagenomic DNA was extracted from bulk soil, rhizospheric soil, and surrounding soil of every biological replicate using PowerSoil DNA Isolation Kit, and examined by using 1% agarose gel electrophoresis.Then an improved dual-index high-throughput sequencing approach with paired-end 250 nt was used to create the sequencing library of 16S rRNA gene (16S rDNA) V3-V4 region.