CoxI amplicons of Sceloporus grammicus fece's. Adult female.
397 base pairs of the coxI were amplified with the primers mICOIintF and jgHCO2198. Primers used contained the adapter for sequencing platform and 8 nt barcodes. Amplification reactions were done in quadruplicate, pooled and purified using QIAquick PCR purification kit according to manufacturers instructions. The quantification of the PCR products was done using NanoDrop 3300 fluorospectrometer with PicoGreen dsDNA assay and combined in equimolar quantities for sequencing.