GSM4116717: Winnie/APCmin_rep2; Mus musculus; RNA-Seq
Total RNA was isolated from ACF macrodissected by using RNeasy FFPE kit (Qiagen) according to the manufacturer instructions. Indexed libraries were prepared from 20ng/ea purified RNA with TruSeq RNA Exome Sample Prep Kit (Illumina) according to the manufacturer's instructions. Libraries were quantified using the TapeStation 4200 (Agilent Technologies) and Invitrogen Qubit fluorometer (Thermo Fisher Scientific), then pooled such that each index-tagged sample was present in equimolar amounts, with final concentration of the pooled samples of 2nM.
GEO Accession
GSM4116717