Alimentary tracts of pea aphid clone P123  replicate 3
Dissected organs were soaked in RNA later (QIAGEN) to avoid RNA degradation and pooled in batches before RNA extraction. Approx 20 alimentary tracts  were pooled for one replicate of RNA extraction by NucleoSpin RNA XS (Macherey-Nagel). rRNA depletion, single stranded-RNA library preparation, multiplexing, and sequencing were performed by Genewiz (NJ, USA)