GSM3601775: O_punctata_60d_RNASEQ; Oryza punctata; RNA-Seq
In brief, total RNA was extracted using TRIzol buffer with 4g fresh tissue from the same plant which was used for ChIP experiment. Then RNA quality was validated by Agilent Bioanalyzer and samples with good quality were purified by RNA Purification Beads and submitted to BGI (shenzhen) for RNASEQ library construction RNASEQ library was constructed with TruSeq RNA Sample Prep Kit (Illumina) according to the standard protocol. Six RNASEQ libraries were adjusted and sequenced in the same lane with proper barcodes with pair end 90bp strategy.
GEO Accession
GSM3601775