GSM1602610: TMT3; Apis mellifera; RNA-Seq
Eviscerated abdomens with attached fat bodies from individual bees were dissected in ice-cold RNAlater® (Qiagen, Valencia, CA) and excess RNAlater® was removed from the samples.  RNA was isolated using TRIzol® Reagent (Invitrogen, Carlsbad, CA). Samples were then treated with TURBO™ DNase (Ambion, Austin, TX) to remove DNA contamination. DNA was isolated from the fat bodies from a second set of bees using a Gentra® Puregene® kit (Qiagen, Valencia, CA).  DNA quality was assessed by electrophoresis, using 1% Agarose gel, and the concentration was determined using a Qubit® 2.0 Fluorometer (Invitrogen, Carlsbad, CA). RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM1602610