Genomics of lone star tick (Amblyomma americanum): Raw sequence reads
Extracted DNA was sent to the Cornell University Biotechnology Resource Center (BRC), where genotyping-by-sequencing (GBS) and a corresponding bioinformatics pipeline for SNP discovery were first developed (Elshire et al. 2011; Lu et al. 2013). BRC scientists optimized the GBS protocol for the lone star tick. The restriction enzyme PstI was used to reduce genome complexity, and unique barcodes were ligated to the digested DNA to construct a sequencing library. The resulting library was single-end sequenced in 100-bp reads on the Illumina HiSeq 2000.