Sediment metagenome of sediment microbial fuel cell: with hydric soil
Bacterial genomic DNA was extracted from 0.5 g of soil by using UltraClean soil DNA isolation kit (MO BIO Laboratory, Carlsbad, CA, USA), according to the manufacturers instruction. 16S rRNA genes of distinct regions (V3-V4) were amplified. Sequencing libraries were generated using TruSeqR DNA PCR-Free Sample Preparation Kit (Illumina, USA) following manufacturer's recommendations and index codes were added. The library quality was assessed on the Qubit 2.0 Fluorometer (Thermo Scientific) and Agilent Bioanalyzer 2100 system. At last, the library was sequenced on an Illumina HiSeq 2500 platform and 250 bp paired-end reads were generated.