RNA-seq of mus musculus: neonatal cardiomyocyte
After treated with PBS or Mydgf recombinant protein for 16 hours, neonatal primary CMs were harvested, and the total RNA was extracted by RNeasy mini kit (QIAGEN). The quantity and quality of total RNA were measured by Nanodrop 2000 and Agilent Bioanalyzer 2100, respectively. The total RNA of 1 g with a RIN value no less than 8.0 for each sample was used for library preparation. cDNA libraries were prepared and sequenced on an Illumina Miseq using the paired-end run methodology at Metagenomics Co. The raw reads were generated, and quality control determination was performed using standard protocols.