GSM1243297: Q77; Homo sapiens; RNA-Seq
Total RNA was extracted from the frozen tumor samples and activated B-cells using TRIzol and according to the protocol described by the supplier (Invitrogen, Carlsbad, CA, USA). Sequencing adaptors were ligated to total RNA on both ends of the RNA molecules and reverse-transcribed. The cDNA was pre-amplified with PCR primers containing specific sequence tags making the libraries compatible with the Illumina flow cells (Illumina, Son en Breugel, The Netherlands). Polyacrylamide gel electrophoresis size selection was performed, excising the 95-105 bp band containing the adaptor-ligated microRNA insert. Quantification and size verification was performed using a high sensitivity DNA chip (Agilent, Amstelveen, The Netherlands).
GEO Accession
GSM1243297