RNA-Seq of maize hybrid Zheng58/Huangzaosi under 67500 plants/ha
Total RNA was extracted from leaves using TRIzol  Reagent (Invitrogen, Carlsbad, CA, USA) following manufacturer's instructions. Oligo (dT) magnetic beads (Illumina, San Diego, CA, USA) were used to get purified mRNA. cDNA libraries were constructed by the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina). Transcriptome sequencing was performed using Illumina HiSeq X Ten according to the manufacturer's protocol.