GSM3380668: 3990-DS-0006; Mus musculus; OTHER
Total RNA was purified from excised tumors using the RNeasy Mini kit (Qiagen). RNA quality was assessed using Bioanalyzer 2100 (Agilent) and final concentration determined by Qubit (Life Technologies). T-cell receptor (TCR) sequencing was performed using the Mouse TCR beta, MTBIvc-X-P bulk kit from iRepertoire (Huntsville, AL). 500 ng of RNA was reverse transcribed using a One-Step reverse transcription and amplification kit (Qiagen). The PCR product was purified using Ampure XP magnetic beads (Agencourt), and amplified again (TopTaq PCR Kit, Qiagen), to add Illumina TruSeq adaptor sequences. Libraries were purified with Ampure XP magnetic beads.
GEO Accession
GSM3380668