RAD-seq of Miscanthus sacchariflorus: RU2012-047: Sample RU2012-047
"Whole genomic DNA of Miscanthus sacchariflorus was digested with PstI and MspI and ligated to adapters such that the barcode (ACGTCA for this sample) was adjacent to the PstI cut site.  After pooling of 95 barcoded samples, size selection from 200-500 bp was performed on a 2% agarose gel.  The library was then amplified with KAPA Hi-Fi polymerase.  Sequencing was performed beginning at the barcoded PstI end of fragments.  A custom Python program was used to split the output into multiple FASTQ files by barcode and remove barcode and adapter sequence."