Ammonia unamended soil from 56-days aerobic dynamic: day 1
This paired-end SRA file contains the V1-V3 16S rRNA raw sequences as a multiplexed library from the 18 ammonia unamended soil samples for Day 1 of the 56-days aerobic dynamic. The paired-end fastq files must be fed into the split_libraries_fastq.py script to demultiplex each Sample by its 16-bp unique barcode. The mapping file to be used is the following; where '$' must be changed to tabulation ('\t') and '@' to a new line ('\n'): SampleID$BarcodeSequence$LinkerPrimerSequence$ReversePrimer$soil_use$dinamic$Practice$Fertilizer$Day$Treatment$Pract.Day$Pract.Fertil.Day$Day.Fertilizer$Soil$OH$Description@DNA163$TAAGGCGATAGATCGC$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$unamended$CT$0$1$CT0$CT.1$CT.0.1$D1.0$111$1$Soil.CTRL.19@DNA164$TAAGGCGACTCTCTAT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$unamended$CT$0$1$CT0$CT.1$CT.0.1$D1.0$211$2$Soil.CTRL.20@DNA165$TAAGGCGATATCCTCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$unamended$CT$0$1$CT0$CT.1$CT.0.1$D1.0$311$3$Soil.CTRL.21@DNA166$TAAGGCGAAGAGTAGA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$unamended$CT$300$1$CT300$CT.1$CT.300.1$D1.300$115$4$Soil.CTRL.22@DNA167$TAAGGCGAGTAAGGAG$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$unamended$CT$300$1$CT300$CT.1$CT.300.1$D1.300$215$5$Soil.CTRL.23@DNA168$TAAGGCGAACTGCATA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$unamended$CT$300$1$CT300$CT.1$CT.300.1$D1.300$315$6$Soil.CTRL.24@DNA169$TAAGGCGAAAGGAGTA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBB$0$1$PBB0$PBB.1$PBB.0.1$D1.0$121$7$Soil.CTRL.25@DNA170$TAAGGCGACTAAGCCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBB$0$1$PBB0$PBB.1$PBB.0.1$D1.0$221$8$Soil.CTRL.26@DNA171$CGTACTAGTAGATCGC$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBB$0$1$PBB0$PBB.1$PBB.0.1$D1.0$321$9$Soil.CTRL.27@DNA172$CGTACTAGCTCTCTAT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBB$300$1$PBB300$PBB.1$PBB.300.1$D1.300$125$10$Soil.CTRL.28@DNA173$CGTACTAGTATCCTCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBB$300$1$PBB300$PBB.1$PBB.300.1$D1.300$225$11$Soil.CTRL.29@DNA174$CGTACTAGAGAGTAGA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBB$300$1$PBB300$PBB.1$PBB.300.1$D1.300$325$12$Soil.CTRL.30@DNA175$CGTACTAGGTAAGGAG$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBC$0$1$PBC0$PBC.1$PBC.0.1$D1.0$151$13$Soil.CTRL.31@DNA176$CGTACTAGACTGCATA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBC$0$1$PBC0$PBC.1$PBC.0.1$D1.0$251$14$Soil.CTRL.32@DNA177$CGTACTAGAAGGAGTA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBC$0$1$PBC0$PBC.1$PBC.0.1$D1.0$351$15$Soil.CTRL.33@DNA178$CGTACTAGCTAAGCCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBC$300$1$PBC300$PBC.1$PBC.300.1$D1.300$155$16$Soil.CTRL.34@DNA179$AGGCAGAATAGATCGC$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBC$300$1$PBC300$PBC.1$PBC.300.1$D1.300$255$17$Soil.CTRL.35@DNA180$AGGCAGAACTCTCTAT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBC$300$1$PBC300$PBC.1$PBC.300.1$D1.300$355$18$Soil.CTRL.36