GSM3142003: Patient_3_Cell_line; Homo sapiens; RNA-Seq
Illumina total RNAseq with ribo-zero depletion The RNeasy Mini kit (Qiagen, cat # 74104) was used for extracting RNA from both 2D cell lines and organoids. Approximately 3 million cells and 1 million cells were used as starting material for 2D and organoids, respectively. A GI pathologist circled areas of high tumor cellularity on H&E sections of the tumor and PDX. RNA was extracted from the same area of an adjacent section using RNeasy FFPE kit (Qiagen, cat # 73504) after treating with Deparaffinization Solution (Qiagen, cat # 19093) RNA library preparation and sequencing: Exome capture was carried out using the RNA access kit (Illumina, cat # RS-301-2001) for the majority of samples
GEO Accession
GSM3142003