nostress_CLN1DCLN3D_R4_070
Samples were taken over 150 min (60 min every 5 min, afterwards every 10 min) and frozen in liquid nitrogen. RNA was extracted with Nucleospin 96 RNA Kit with small modifications. Cell lysis was done for 30 min at 30C by adding 450l of lysis buffer containing 1M sorbitol, 100mM EDTA, and 0.45ml lyticase (10IU/ml). The rest of the RNA extraction was performed according to manufacturer's details. Extracted RNA was converted to cDNA, barcoded and sequenced with Illumina HiSeq 2500