Microbe sample from Escherichia coli mutant Flu100ppm-30D chl-4
The sample was sequenced following the  1D genomic DNA selecting for long reads using SQK LSK108  protocol, which was similar to the 2D protocol, but with the use of Ampure XP beads instead of MyOne Streptavidin C1 beads after the ligation of the adaptors. The protocols were followed according to the manufacturer s instructions except for the following steps: incubation time of 20 minutes in each end repair and ligation steps, 0.7x AMPure XP beads for purification, and warm up elution buffer at 50 C.