GSM4272648: d7 KO CT rep1; Mus musculus; RNA-Seq
mRNA was prepared from ears of Aldara-treated mice with the mirVana kit (InVitrogen #AM1560, ThermoFisher Scientific) following the manufacturer's instructions. RNA clean-up was performed with RNA Clean XP beads (Beckman Coulter #A66514, Indianapolis, IND, USA). RNA quantification was performed with a Qubit fluorimeter (Life Technologies, Thermo Fisher Scientific) and RNA integrity assessed with a Bioanalyzer (Agilent Technologies, Santa Clara, CAL, USA). The TruSeq mRNA stranded kit from Illumina was used for the library preparation with 500 ng of total RNA as input. The 34 and 40 libraries were pooled at 2 nM and loaded for clustering on 4 and 5 lanes respectively of a single-read Illumina Flow cell. Reads of 50 bases were generated using the TruSeq SBS chemistry on an Illumina HiSeq 4000 sequencer. Libraries were sequenced using single-end reads (100 nt-long) on an Illumina HiSeq4000.
GEO Accession
GSM4272648