GSM2212647: 11W rightcolon female control S26; Homo sapiens; RNA-Seq
RNA was prepared using the KAPA Stranded RNA-Seq with RiboErase sample preparation kit (kapabiosystems) in accordance with the manufacturer’s instructions. Briefly, 500ng of total RNA was ribo depleted, fragmented and went under first and second strand synthesis, A tailing, adapter ligation and PCR amplification (using 11 cycles).  Final libraries were quantified using the KAPA Library Quantification Kit (KAPA Biosystems), Qubit Fluorometer (Life Technologies) and Agilent 2100 BioAnalyzer. Samples were sequenced on an Illumina HiSeq2500 sequencer (v4 chemistry) using 1 x 100bp cycles.
GEO Accession
GSM2212647