GSM4272587: SR-74_inf; Homo sapiens; RNA-Seq
Dataset consists of a total of six single-cell datasets generated using the 10x Genomics Chromium platform. Prior to GEM generation, the sample's concentration (cells/ul) and viability (%) were determined by staining the cells with trypan blue and by manually counting using an inverted microscope. Next-generation sequencing libraries were prepared following the 10X Single Cell v2 protocol without modification. Sample quality was evaluated using Agilent's Bioanalyzer High Sensitivity reagent kit following cDNA amplification and library construction. The completed libraries were quantified using the Kapa Library Quantification Kit and the Life Technologies Viia7 Real-time PCR instrument. Libraries were diluted to 2nM and sequenced in a paired-end, single-indexed format on the Illumina HiSeq2500 using the Rapid Run v2 chemistry. Cells were sequenced at a depth of 50.000 reads/cell.
GEO Accession
GSM4272587