GSM2649282: LY2_shSRC1_BA_MeDIP; Homo sapiens; MeDIP-Seq
DNA was extracted with average concentrations of 370 ng/µl determined by Qubit Fluorometer, micro plate reader, and nanodrop. The extracted DNA achieved a quantity of above the cut off of ≥ 5µg for all samples. Genome DNA was fragmented to 100-500bp by sonication. DNA-end was repaired to overhang a 3'-dA, then adapters were ligated to the end of DNA fragments. Double-stranded DNA was denatured. Then DNA fragments were immunoprecipitated by 5mC antibody. Real-time PCR was used to validate the quality of immunoprecipitation. DNA fragments with proper size (usually 200-300bp, including adaptor sequence) were selected after PCR amplification. Then there was adequate library for sequencing.
GEO Accession
GSM2649282