mRNA library of an enriched Lenisia limosa culture with bacterial symbionts
Total RNA was extracted following the protocol of Smith et al. (2007, AEM 73:3612-3622) with minor modifications. Extracted RNA was treated with RQ1 DNase (Promega), purified with RNeasy MinElute columns (Qiagen) and stored in TE buffer at -80°C. Approx. 1 µg of total RNA was used for preparation of an mRNA sequencing library following the illumina TruSeq RNA Sample Preparation v2 Guide, using poly-T oligo-attached magnetic beads to enrich eukaryotic mRNA.