Escherichia coli O157 whole genome sequencing
Libraries were generated using 1 µg of the gDNA and enzyme fragmented using the Ion XpressPlus Fragment Library Kit. Specifically, gDNA was fragmented to 200-300bp blunt-ended DNA fragments. The fragmented DNA was ligated to Ion-compatible barcoded adapters, followed by nick-repair to complete the linkage between adapters and DNA inserts. The adapter-ligated library was then size-selected for optimum length (330bp) and the final library was amplified