GSM1873361: Control; Mus musculus; RNA-Seq
Non-adherent cells were harvested and cultured with M-CSF (30 ng/ml) for 3 days. Floating cells were removed and adherent cells were used as bone marrow-derived macrophage (BMM) cells. On day 3, the cells were stained for tartrate-resistant acid phosphatase (TRAP). The TRAP-positive multinucleated cells containing three or more nuclei are referred as osteoclasts. RNA extracted from cells was polyA+ labelled and used to generate libraries following the manufacturer’s protocols (Illumina). The modified cDNA molecules were subjected to sequencing by Illumina Hiseq 2500 system
GEO Accession
GSM1873361