Complete genome sequencing of S. paucimobilis AIMST S2
Genomic DNA extraction was performed according to the GeneJet Genomic DNA purification kits protocol. The concentration and quality of extracted DNA was determined using Nanodrop  Lite spectrophotometer (Thermo Scientific, Wilmington, DE, USA), Qubit dsDNA BR assay (Thermo Fisher Scientific, Wilmington, DE, USA) and agarose gel electrophoresis. Genomic DNA was then fragmented using Covaris to a targeted size of 350bp and upon adapter ligation, a library containing fragments of 470bp  was generated. The library size was determined using Bioanalyzer high sensitivity DNA chip. Paired-end DNA sequencing was performed with Illumina HiSeq 2500.