GSM3230432: tumor C3-1 basal SOX10 high sample5 (RNA-seq); Mus musculus; RNA-Seq
The normal cells were dissected and FACS isolated from primary mammary tissue. The PY230 cells were transplanted into syngeneic mice to form tumors, and the tumors were dissected and FACS isolated. The C3-1-Tag cells were dissected and FACS isolated from spontaneous tumors. See the Methods section of the manuscript for details of isolation and FACS markers. The RNA-seq samples were prepared with the SmartSeq2 protocol. Around 2000 freshly dissected and sorted cells from each cell type were processed. Full-length cDNA were generated, and their size distribution was checked with TapeStation to ensure good RNA quality.  The cDNA were then amplified with 18 PCR cycles, tagmentated and amplified again with 10 PCR cycles using Nextera XT kit (Illumina FC-131-1096). The sequencing library was purified with AMPure XP beads. 50 bp or 75 bp single-end sequencing was performed with Illumina HiSeq 2500 SmartSeq2 (Picelli et al., 2014)
GEO Accession
GSM3230432