MiSeq (short) RNAseq library of T. pityocampa egg mass
RNA was extracted using a Trizol extraction procedure. After quality control and purification, RNAs from 2 to 5 individuals were pooled in equimolar proportions for each developmental stage. Libraries were constructed with the TruSeq kit and an insert size of 450bp. After purification, the RNAs were fragmented and reverse transcribed. Ligated cDNAs were amplified through 15 PCR cycles. The libraries were validated with an Agilent Bioanalyzer and sequenced on an Illumina MiSeq sequencer (2x300bp).