Illumina HiSeq 2000 paired end sequencing; Arabidopsis col-0 WT strand-specific RNA-Seq data from poly-A pulldown
Arabidopsis col-0 WT strand-specific RNA-Seq data from poly-A pulldown
Harvesting of whole seedlings. Briefly, the RNA-Seq data for Experiments 1 is wild-type (WT) Arabidopsis thaliana Colombia-0 (Col-0) biological replicates. WT A. thaliana Col-0 seeds were sown aseptically on MS10 plates. The seeds were stratified for 2 days at 4C and then grown at a constant 21C under a 16-h light/8-h dark cycle for a further 14 days, at the end of which the seedlings were harvested. Total RNA was isolated from the seedlings with the RNeasy Plant Mini Kit (Qiagen). In Experiment 1, DNAse digestion was performed on column, as a part of RNA isolation, and 8 μl of ERCC spike-ins (Exter-nal RNA Controls Consortium 2005) at a 1:100 dilution was added to 4 μg of total RNA. Libraries were prepared according to the TruSeq® Stranded mRNA Sample Preparation Guide Rev E.
Experimental Factor: replicate
3