GSM3474201: Exd+Hth_233 (transient); Drosophila melanogaster; ChIP-Seq
Cells were harvested and fixed using 1% formaldehyde in PBS for 10 min at 23°C in an Eppendorf Thermomixer at 700 rpm, then sorted using a MoFlo FACS machine (Beckman Coulter) to standardise levels of Hox-GFP proteins across experiments, and finally sonicated to provide chromatin fragments of approximately 500 bp in length. Fragmented chromatin was immunoprecipitated using an anti-GFP antibody (SIGMA, G1544) and DNA was purified using a standard phenol-chloroform extraction. Sequencing libraries were prepared using the SMARTer ThruPLEX DNA-seq Kit (Takara Bio Inc.) in accordance with the sample preparation guide.
GEO Accession
GSM3474201