GSM3597749: BJfibroblastsctrl_RNA_rep1; Homo sapiens; RNA-Seq
Snapfrozen RNA samples were further extracted using chloroform and QIAGEN RNeasy Mini Kit (#74106) including DNase digestion. Purified RNA was then reverse transcribed to cDNA using High Capacity cDNA Reverse Transcription Kit from Applied Biosystems (4368814), cDNA was then quantified and used to construct sequencing libraries. Poly(A) + RNA was isolated using double selection with poly-dT beads, followed by first and second-strand synthesis.
GEO Accession
GSM3597749