GSM4547459: 16M_P; Danaus plexippus; RNA-Seq
The frozen tissue was thawed, and then total RNA was harvested using Trizol reagent following Qiagen RNeasy protocol. RNA concentration and RNA IQ (integrity and quality) score were determined using a Qubit 4 Fluorometer (Thermo Fischer). Illumina TruSeq Stranded mRNAseq Prep Kit was used with 100 ng of total RNA for the construction of sequencing libraries, following the manufactor's protocols.
GEO Accession
GSM4547459