Ammonia amended soil from 56-days aerobic dynamic: day 3
This paired-end SRA file contains the V1-V3 16S rRNA raw sequences as a multiplexed library from the 18 ammonia amended soil samples for Day 3 of the 56-days aerobic dynamic. The paired-end fastq files must be fed into the split_libraries_fastq.py script to demultiplex each Sample by its 16-bp unique barcode. The mapping file to be used is the following; where '$' must be changed to tabulation ('\t') and '@' to a new line ('\n'): SampleID$BarcodeSequence$LinkerPrimerSequence$ReversePrimer$soil_use$dinamic$Practice$Fertilizer$Day$Treatment$Pract.Day$Pract.Fertil.Day$Day.Fertilizer$Soil$OH$Description@DNA37$TCCTGAGCTAGATCGC$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$amended$CT$0$3$CT0$CT.3$CT.0.3$D3.0$111$25$Soil.NH4.37@DNA38$TCCTGAGCCTCTCTAT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$amended$CT$0$3$CT0$CT.3$CT.0.3$D3.0$211$26$Soil.NH4.38@DNA39$TCCTGAGCTATCCTCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$amended$CT$0$3$CT0$CT.3$CT.0.3$D3.0$311$27$Soil.NH4.39@DNA40$TCCTGAGCAGAGTAGA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$amended$CT$300$3$CT300$CT.3$CT.300.3$D3.300$115$28$Soil.NH4.40@DNA41$TCCTGAGCGTAAGGAG$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$amended$CT$300$3$CT300$CT.3$CT.300.3$D3.300$215$29$Soil.NH4.41@DNA42$TCCTGAGCACTGCATA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$amended$CT$300$3$CT300$CT.3$CT.300.3$D3.300$315$30$Soil.NH4.42@DNA43$TCCTGAGCAAGGAGTA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBB$0$3$PBB0$PBB.3$PBB.0.3$D3.0$121$31$Soil.NH4.43@DNA44$TCCTGAGCCTAAGCCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBB$0$3$PBB0$PBB.3$PBB.0.3$D3.0$221$32$Soil.NH4.44@DNA45$TAGGCATGTAGATCGC$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBB$0$3$PBB0$PBB.3$PBB.0.3$D3.0$321$41$Soil.NH4.45@DNA46$TAGGCATGCTCTCTAT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBB$300$3$PBB300$PBB.3$PBB.300.3$D3.300$125$42$Soil.NH4.46@DNA47$TAGGCATGTATCCTCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBB$300$3$PBB300$PBB.3$PBB.300.3$D3.300$225$43$Soil.NH4.47@DNA48$TAGGCATGAGAGTAGA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBB$300$3$PBB300$PBB.3$PBB.300.3$D3.300$325$44$Soil.NH4.48@DNA49$TAGGCATGGTAAGGAG$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBC$0$3$PBC0$PBC.3$PBC.0.3$D3.0$151$45$Soil.NH4.49@DNA50$TAGGCATGACTGCATA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBC$0$3$PBC0$PBC.3$PBC.0.3$D3.0$251$46$Soil.NH4.50@DNA51$TAGGCATGAAGGAGTA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBC$0$3$PBC0$PBC.3$PBC.0.3$D3.0$351$47$Soil.NH4.51@DNA52$TAGGCATGCTAAGCCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBC$300$3$PBC300$PBC.3$PBC.300.3$D3.300$155$48$Soil.NH4.52@DNA53$CTCTCTACTAGATCGC$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBC$300$3$PBC300$PBC.3$PBC.300.3$D3.300$255$49$Soil.NH4.53@DNA54$CTCTCTACCTCTCTAT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBC$300$3$PBC300$PBC.3$PBC.300.3$D3.300$355$50$Soil.NH4.54