GSM3141555: HFY114 WT-rep2; Saccharomyces cerevisiae; RNA-Seq
Total RNA was extracted from yeast cells using hot phenol. Total RNA was treated with Baseline-zero DNase (Epicenter, cat# DB0711K) to remove any genomic DNA contamination. Five micrograms of DNase-treated total RNA was then depleted of rRNAs using the Illumina yeast RiboZero Removal Kit (cat# MRZY1306) and the resulting RNA was used for RNA-Seq library preparation. Multiplex strand-specific cDNA libraries were constructed using the Illumina TruSeq Stranded mRNA LT Sample Prep Kit (cat# RS-122-2101).
GEO Accession
GSM3141555