GSM2054803: ChIPSeq_KO_P13_H3K27me3; Mus musculus; RNA-Seq
ChIP-Seq: Frozen-stored mammary tissues from virgin or p13 were broken into powder with mortar and pestle. Nuclei were extracted in Farnham lysis buffer (5mM PIPES pH8.0, 85mM KCl, 0.5% NP-40 and protease inhibitors). Antibodies against KDM6A (Sigma, HPA002111), NFIB (Santa Cruz, sc-5567), ERα (Santa Cruz, sc-543X), PR (Santa Cruz, sc-7208X) and H3K4me1 (Abcam, ab-8895) were used for ChIP. Libraries were prepared for sequencing using standard Illumina protocols.
GEO Accession
GSM2054803