GSM1606459: Ark_WT_TGFb_1hr [HS_A2]; Mus musculus; RNA-Seq
Total RNA was extracted using a Nucleospin RNA kit (Clontech) with in-column DNase digestion. The RNA quality was verified to have an RNA integrity number (RIN) greater than 7.5 through using a Bioanalyzer (Agilent). Stranded mRNA-seq (mRNA sequencing) libraries were prepared using the TruSeq Stranded mRNA Sample Prep Kit (Illumina). mRNA was selected by poly(dT) magnetic beads and then fragmented and reverse-transcribed; cDNA was end-repaired, adenylated, adapter-ligated and PCR amplified.
GEO Accession
GSM1606459