High throught sequencing-microbiome- Rf larvae Cocos nucifera
The variable region 4 (V4) of the bacterial 16S rRNA gene was amplified with the general 16S rRNA primers 515F (5’-GTGYCAGCMGCCGCGGTA-3’) and 806R (5’-GGACTACHVGGGTWTCTAAT-3’). The PCR reactions were carried out in a total volume of 30 µl which comprised of 15 µl of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 µM of forward and reverse primers and 10 ng of template DNA.