GSM1923403: HepG2-p53-miRNA-seq rep 2; Homo sapiens; miRNA-Seq
Total RNA was extracted from the cultured cells using Trizol Reagent (Invitrogen) according to the manufacturer’s instructions.Length of 18–30 nt small RNAs were isolated from total RNA using 15% denaturing polyacrylamide gel electrophoresis. Small RNAs were ligated to a 5'adaptor and a 3'adaptor by T4-RNA ligase and reverse-transcribed to cDNA. Samples were treated with Dox for 0h and 24h twice, four libraries were obtained marked as HepG2 0h-1, HepG2 24h-1, HepG2 0h-2, HepG2 24h-2. Agilent 2100 Bioanalyzer and ABI StepOnePlus Real-Time PCR System were applied to test the quality, purity and concentrations of the samples. Finally, cDNA libraries were sequenced using Illumina HiSeq 2000 platform (Illumina, San Diego, CA, USA) by following the manufacturer’s instructions.
GEO Accession
GSM1923403