w1d2
strand-specific paired-endStrand-specific libraries were prepared from the extracted RNA using the Illumina TruSeq Stranded mRNA reagent kit. Samples were barcoded at the library preparation stage for multiplexing. Sequencing was performed by Illumina HiSeq 2000/2500 in 100 nucleotide paired-end mode. Fastq files were produced from the raw data using v1.82 of the Illumina Analysis software.