GSM2651029: Δhlx2 mutant 1; Halobacterium salinarum NRC-1; RNA-Seq
Total RNA was extracted using Absolutely RNA Miniprep kit (Agilent) per manufacturer's instructions RNA was rRNA depeleted using Ribo-Zero kit (Illumina) per manufacturer's instructions. Depleted RNA was converted to cDNA libraries using Stranded RNA library preparation kit from Kapa Biosystems per manufacturer's instructions including 10 rounds of amplification and ligation to Illumina TruSeq adapters 1-6. Quality was determined using a High Sensitivity DNA chip on a Bioanalyzer 2100 (Agilent), pooled, and run on a single lane in an Illumina HiSeq 2500
GEO Accession
GSM2651029