GSM2051886: Clone 34 day5 Replicate1; Homo sapiens x Mus musculus hybrid cell line; RNA-Seq
Total RNA was exacted by RNABee (Amsbio), treated with RNAse-free DNaseI (Ambion) and cleaned-up with RNAeasy Mini-elute columns. RNA-seq libraries were prepared from 0.6μg total RNA accordingly to Illumina True-seq RNA sample preparation v2 guide (Illumina). For the ligation step, 2 μl of adaptors were used. Libraries were amplified 14 cycles before deep-sequencing.
GEO Accession
GSM2051886