GSM4274351: greA-1 [RNA-seq]; Mycolicibacterium smegmatis MC2 155; RNA-Seq
Total RNA was prepared from wild-type and mutant strains using TRIzol reagent (Invitrogen), followed by DNase I treatment. Approximately 1-μg total RNA samples were treated by the Ribo-Zero rRNA removal procedure (Illumina) to enrich for mRNA. Approximately 1μg of RNA was used for library preparation using a ScriptSeq (v2) RNA-seq kit and high-throughput sequencing on an Illumina HiSeq platform.
GEO Accession
GSM4274351