GSM2990697: Stage 2 rep3 [mRNA]; Bos taurus; RNA-Seq
RNA was isolated from a total of 20 EVs samples (5 replicates, 4 stages) using RNeasy Micro kit (QIAGEN) according to the manufacturer's instructions. RNA quality and concentration was evaluated by Agilent 2100 Bionalyzer (Agilent Technologies, Santa Clara, CA.) and NanoDrop (ThermoFisher). Poly(A) RNA was isolated starting from of 450 ng of total RNA by the use of NEXTflex™ Poly(A) beads. Poly(A) RNA was used for the preparation of mRNA libraries with the NEXTflex™ Rapid Directional qRNA-Seq Kit (Bioo Scientific). Library preparation followed the manufacturer's instructions.
GEO Accession
GSM2990697