GSM3231353: WGBS_J1-ESC; Mus musculus; Bisulfite-Seq
Genomic DNA was purified from mESCs and NSCs using standard phenol-chloroform-isoamyl alcohol extraction followed by ethanol precipitation. Genomic DNA was spiked with 0.5% lambda DNA, fragmented to 250-350 bp using Bioruptor (Diagenode), and concentrated by ethanol precipitation. 500 ng of fragmented DNA was used for bisulfite conversion using EZ DNA Methylation-Direct Kit (Zymo). WGBS libraries were constructed using Accel-NGS Methyl-Seq DNA Library Kit (Swift) according to manufacturer's recommendations. WGBS libraries were quantified using Qubit Fluorometer and Agilent 2200 TapeStation (Agilent). Libraries were pooled and sequenced using the Illumina HiSeq 4000 as 150-bp paired-end sequencing reads.
GEO Accession
GSM3231353