GSM2649341: BCRABL_MINUS_SHP2_WT_REP_0; Mus musculus; RNA-Seq
RNA (150 ng) from each sample was reverse-transcribed into cDNA using the Illumina TruSeq Stranded mRNA kit. cDNA libraries were size-indexed and validated using an Agilent Bioanalyzer, and concentrations were confirmed by qPCR. For the 16 samples, each of the 4 libraries (1 WT SHP2+/+, 1 WT SHP2-/-, 1 BCR-ABL1 SHP2+/+, and 1 BCR-ABL1 SHP2-/-) was loaded onto an Illumina cBot for cluster generation, and the flow cell was subjected to 100 cycles of paired-end sequencing on an Illumina HiSeq 2000.
GEO Accession
GSM2649341