GSM4485334: Non specific 6; Homo sapiens; RNA-Seq
FFPE esophageal biopsy tissue blocks were shipped from each center to the Swiss EoE Clinic. Esophageal biopsies from patients with classical EoE (10 samples), EoE-like esophagitis (13 samples), non-specific esophagitis (10 samples), lymphocytic esophagitis (5 samples), and esophagus-healthy individuals (7 samples) were processed. Automated RNA Extraction from FFPE was performed using the Maxwell® RSC RNA FFPE Kit in Maxwell® RSC Instrument (Promega, Dübendorf, Switzerland). RNA-seq libraries were prepared using SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara Bio USA, Inc., CA, USA) and were sequenced in a NovaSeq S1 flow cell (2 x 50 bp) (Illumina, CA, USA) as previously described. RNA sequencing was performed at the RNAseq core (Next Generation Sequencing (NGS) Platform) of the University of Bern.
GEO Accession
GSM4485334