GSM2991379: IVBL rep2; Homo sapiens; RNA-Seq
After homogenization, total RNA were extracted by Direct-zol RNA miniprep kit. . The first and second strand cDNA were prepared by SuperScript III First-Strand Synthesis System and NEBNext mRNA Second Strand Synthesis Module from at least 200ng total RNA for each sample. Illumina Nextera XT DNA Sample Prep Kit  was used with 1 ng of dsDNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using nextera Illumina protocols
GEO Accession
GSM2991379