Ammonia amended soil from 56-days aerobic dynamic: day 1
This paired-end SRA file contains the V1-V3 16S rRNA raw sequences as a multiplexed library from the 18 ammonia amended soil samples for Day 1 of the 56-days aerobic dynamic. The paired-end fastq files must be fed into the split_libraries_fastq.py script to demultiplex each Sample by its 16-bp unique barcode. The mapping file to be used is the following; where '$' must be changed to tabulation ('\t') and '@' to a new line ('\n'): SampleID$BarcodeSequence$LinkerPrimerSequence$ReversePrimer$soil_use$dinamic$Practice$Fertilizer$Day$Treatment$Pract.Day$Pract.Fertil.Day$Day.Fertilizer$Soil$OH$Description@DNA19$TAAGGCGATAGATCGC$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$amended$CT$0$1$CT0$CT.1$CT.0.1$D1.0$111$1$Soil.NH4.19@DNA20$TAAGGCGACTCTCTAT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$amended$CT$0$1$CT0$CT.1$CT.0.1$D1.0$211$2$Soil.NH4.20@DNA21$TAAGGCGATATCCTCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$amended$CT$0$1$CT0$CT.1$CT.0.1$D1.0$311$3$Soil.NH4.21@DNA22$TAAGGCGAAGAGTAGA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$amended$CT$300$1$CT300$CT.1$CT.300.1$D1.300$115$4$Soil.NH4.22@DNA23$TAAGGCGAGTAAGGAG$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$amended$CT$300$1$CT300$CT.1$CT.300.1$D1.300$215$5$Soil.NH4.23@DNA24$TAAGGCGAACTGCATA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$amended$CT$300$1$CT300$CT.1$CT.300.1$D1.300$315$6$Soil.NH4.24@DNA25$TAAGGCGAAAGGAGTA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBB$0$1$PBB0$PBB.1$PBB.0.1$D1.0$121$7$Soil.NH4.25@DNA26$TAAGGCGACTAAGCCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBB$0$1$PBB0$PBB.1$PBB.0.1$D1.0$221$8$Soil.NH4.26@DNA27$CGTACTAGTAGATCGC$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBB$0$1$PBB0$PBB.1$PBB.0.1$D1.0$321$9$Soil.NH4.27@DNA28$CGTACTAGCTCTCTAT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBB$300$1$PBB300$PBB.1$PBB.300.1$D1.300$125$10$Soil.NH4.28@DNA29$CGTACTAGTATCCTCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBB$300$1$PBB300$PBB.1$PBB.300.1$D1.300$225$11$Soil.NH4.29@DNA30$CGTACTAGAGAGTAGA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBB$300$1$PBB300$PBB.1$PBB.300.1$D1.300$325$12$Soil.NH4.30@DNA31$CGTACTAGGTAAGGAG$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBC$0$1$PBC0$PBC.1$PBC.0.1$D1.0$151$13$Soil.NH4.31@DNA32$CGTACTAGACTGCATA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBC$0$1$PBC0$PBC.1$PBC.0.1$D1.0$251$14$Soil.NH4.32@DNA33$CGTACTAGAAGGAGTA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBC$0$1$PBC0$PBC.1$PBC.0.1$D1.0$351$15$Soil.NH4.33@DNA34$CGTACTAGCTAAGCCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBC$300$1$PBC300$PBC.1$PBC.300.1$D1.300$155$16$Soil.NH4.34@DNA35$TCCTGAGCTAGATCGC$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBC$300$1$PBC300$PBC.1$PBC.300.1$D1.300$255$25$Soil.NH4.35@DNA36$TCCTGAGCCTCTCTAT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$amended$PBC$300$1$PBC300$PBC.1$PBC.300.1$D1.300$355$26$Soil.NH4.36