GSM3597568: RNASEQ-WT-1; Homo sapiens; RNA-Seq
Ribosome Profiling was performed as described in Baudin-Baillieu et al, 2016 and total RNA was purified by hot acid phenol extraction cDNA library from 100 ng RNA was prepared by the Imagif plateform (Institut de Biologie Intégrative de la Cellule, Gif sur Yvette, France), using the TruSeq Small RNA Sample Prep Kit with 3' sRNA Adapter (Illumina) according to the manufacturer's protocol. RNA integrity and quality was verified using Bioanalyzer Small RNA Analysis kit (Agilent). Sequencing was performed at the genomic plateform of the Institut de Biologie de l'École Normale Supérieure (Paris, France) on an Illumina Nextseq 500 and with a single-read 75 cycle runs. Four sequencing libraries were prepared from purified RPFs and total RNA, for each biological replicate of eRF3-depleted, Upf1-depleted and control cells. A minimum of 7 108 reads were achieved for bioinformatics analysis.
GEO Accession
GSM3597568