Amplicon library of mus musculus: pilot pooled shRNA injected testis: Sample pilot shRNA pool mouse testis 2 (TaxId: 10090)
Custom primers amplified the shRNA sequence off a pLKO plasmid/genomic DNA and attach Illumina MiSeq adapters onto both ends. 4 technical replicates (from unique aliquots of genomic DNA) per testis sample and 4 technical replicates for the injected shRNA pool