GSM2651063: 9-D3-Th2_S9_L001_R1_001; Homo sapiens; RNA-Seq
FACS-sorted Th-like Tregs (2x105 per well) were activated with CD3/CD28 beads (ratio 1:4) for 72h. Cells were lysed in Trizol (Thermo Fisher Scientific) and RNA was isolated with Direct-Zol RNA MicroPrep w/Zymo-Spin columns (Cambridge Bioscience). RNA sequencing was performed using The Human Inflammation & Immunity Transcriptome RNA targeted panel (Qiagen). Samples were quantified with Agilent High Sensitivity DNA Kit (Agilent Technologies) and sequenced with Illumina MiSeq using MiSeq Reagent Kit v3 (150-cycle). 100ng of RNA was used to construct libraries. RNA libraries were prepared for sequencing using standard Illumina protocols provided but with kits procided by Qiagen
GEO Accession
GSM2651063