6YFP
The DNA library construction and sequencing was carried out by BGI in the following way: DNA was sheared to 500 bp fragments by sonication, the ends were made blunt by T4 DNA Polymerase, adenines were added to the 3’ end of DNA fragments, and these were ligated to adapters by T4 DNA ligase. Finally this mixture was enriched for the adapter-modified DNA fragments by PCR and was size selected by gel extraction purification. Each sample was pair-end sequenced on an Illumina HiSeq 2000. Standard procedures produced data sets of Illumina paired-end 90bp read pairs with insert size (including read length) of ~470bp.