Ammonia unamended soil from 56-days aerobic dynamic: day 7
This paired-end SRA file contains the V1-V3 16S rRNA raw sequences as a multiplexed library from the 18 ammonia unamended soil samples for Day 7 of the 56-days aerobic dynamic. The paired-end fastq files must be fed into the split_libraries_fastq.py script to demultiplex each Sample by its 16-bp unique barcode. The mapping file to be used is the following; where '$' must be changed to tabulation ('\t') and '@' to a new line ('\n'): SampleID$BarcodeSequence$LinkerPrimerSequence$ReversePrimer$soil_use$dinamic$Practice$Fertilizer$Day$Treatment$Pract.Day$Pract.Fertil.Day$Day.Fertilizer$Soil$OH$Description@DNA217$TAAGGCGATAGATCGC$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$unamended$CT$0$7$CT0$CT.7$CT.0.7$D7.0$111$1$Soil.CTRL.73@DNA218$TAAGGCGACTCTCTAT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$unamended$CT$0$7$CT0$CT.7$CT.0.7$D7.0$211$2$Soil.CTRL.74@DNA219$TAAGGCGATATCCTCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$unamended$CT$0$7$CT0$CT.7$CT.0.7$D7.0$311$3$Soil.CTRL.75@DNA220$TAAGGCGAAGAGTAGA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$unamended$CT$300$7$CT300$CT.7$CT.300.7$D7.300$115$4$Soil.CTRL.76@DNA221$TAAGGCGAGTAAGGAG$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$unamended$CT$300$7$CT300$CT.7$CT.300.7$D7.300$215$5$Soil.CTRL.77@DNA222$TAAGGCGAACTGCATA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$unamended$CT$300$7$CT300$CT.7$CT.300.7$D7.300$315$6$Soil.CTRL.78@DNA223$TAAGGCGAAAGGAGTA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBB$0$7$PBB0$PBB.7$PBB.0.7$D7.0$121$7$Soil.CTRL.79@DNA224$TAAGGCGACTAAGCCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBB$0$7$PBB0$PBB.7$PBB.0.7$D7.0$221$8$Soil.CTRL.80@DNA225$CGTACTAGTAGATCGC$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBB$0$7$PBB0$PBB.7$PBB.0.7$D7.0$321$9$Soil.CTRL.81@DNA226$CGTACTAGCTCTCTAT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBB$300$7$PBB300$PBB.7$PBB.300.7$D7.300$125$10$Soil.CTRL.82@DNA227$CGTACTAGTATCCTCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBB$300$7$PBB300$PBB.7$PBB.300.7$D7.300$225$11$Soil.CTRL.83@DNA228$CGTACTAGAGAGTAGA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBB$300$7$PBB300$PBB.7$PBB.300.7$D7.300$325$12$Soil.CTRL.84@DNA229$CGTACTAGGTAAGGAG$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBC$0$7$PBC0$PBC.7$PBC.0.7$D7.0$151$13$Soil.CTRL.85@DNA230$CGTACTAGACTGCATA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBC$0$7$PBC0$PBC.7$PBC.0.7$D7.0$251$14$Soil.CTRL.86@DNA231$CGTACTAGAAGGAGTA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBC$0$7$PBC0$PBC.7$PBC.0.7$D7.0$351$15$Soil.CTRL.87@DNA232$CGTACTAGCTAAGCCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBC$300$7$PBC300$PBC.7$PBC.300.7$D7.300$155$16$Soil.CTRL.88@DNA233$AGGCAGAATAGATCGC$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBC$300$7$PBC300$PBC.7$PBC.300.7$D7.300$255$17$Soil.CTRL.89@DNA234$AGGCAGAACTCTCTAT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBC$300$7$PBC300$PBC.7$PBC.300.7$D7.300$355$18$Soil.CTRL.90