CP Cas9
This method was modified from previous Tnseq protocols (Coradetti et al. 2018). Briefly, the transposed plasmids were sheared to ~300bp using a S220 Focused-ultrasonicator (Covaris) and purified in between each following step using Agencourt AMPure XP beads (Beckman Coulter). Following shearing fragments were end repaired and A-tailed according to NEB manufacturers protocols and then universal adapters were ligated on in a 50 ul quick ligase reaction at RT. Finally fragments from each library were amplified in a 20 cycle reaction with Indexed Illumina primers  that annealed upstream of the new CP start codon and in the universal adapter (Supplementary Table S1). PCRs were cleaned again and analyzed for primer dimer via a Agilent Bioanalyzer DNA 1000 chip. Sequencing was performed at the QB3 Vincent J. Coates Genomics Sequencing Laboratory on a HiSeq2500 in a 100bp run.