GSM3597647: ATAC-seq_human primary fetal astrocytes batch 2_rep2; Homo sapiens; ATAC-seq
Cells were harvested, snap frozen in dry ice, and stored at -80C. Cells were washed with ice cold PBS containing protease inhibitor and nuclei were extracted prior to library construction. The standard ATAC-seq protocol was used, with the transposase reaction carried out for 30 minutes at 37C. 8-10 cycles of amplification were performed and dual size selection was performed with Ampure XP beads.
GEO Accession
GSM3597647