GSM1243348: E. coli UDG Predigestion Beseq (normalized); Escherichia coli; OTHER
genomic DNA was isolated using SDS and proteinase K followed by RNAseA treatment and DNA precipitation genomic DNA was treated with Beseq enzymes as stated for samples above.  Samples were treated with NEB end repair kit.  Samples were A-tailed using Klenow exo- from Enzymatics.  Samples were ligated with Illumina Truseq adapters using Enzymatics T4 DNA ligase.  Samples were size selected with Invitrogen size select gels.  Samples were PCR amplified using barcoded Illumina Truseq primers and Ampure bead purified.  Samples were quantitated with Qubit before sequence submission.
GEO Accession
GSM1243348