16S-RNA gene amplicon sequencing of a zebrafish gut metagenome
Sequence libraries of the V1 and V3 region of the 16S rRNA genes from each of the samples were constructed using the universal 16S rRNA primers 27F and 534R. The PCR products were purified and the barcodes were attached in a second PCR using barcoded primers. The purified, barcoded amplicon libraries were quantified, pooled equimolar and prepared for sequencing on a MiSeq DNA Sequencer (Illumina).