E. coli MG1655 with empty pBAD sample1
E. coli MG1655 with empty pBAD was grown in LB to OD600 of 0.4, induced with 0.2% arabinose for 15 minutes. Total RNA was collected using TRI reagent, DNase treated, and rRNA removed using a RiboZero kit. Four Illumina mRNA-seq libraries (two control samples and two test samples) were prepared and multiplexed into a single lane of an Illumina HiSeq 2000 (76 cycles).