GSM1681056: GFP RNAi rep1; Drosophila melanogaster; RNA-Seq
Total RNA was isolated from treated S2 cells using the EZ1 RNA Universal Kit (Quiagen). Cells were disrupted and homogenized in 750 µl  QIAzol lysis reagent via bead-milling on the TissueLyser II (Qiagen). RNA was collected from the homogenate by chloroform exraction and purified on the EZ1 Advanced (Qiagen) with additional step to remove any residual DNA. RNA libraries were prepared for sequencing using standard Illumina protocols