GSM3379517: Control Morpholino replicate 1; Danio rerio; RNA-Seq
single dorsal forerunner cells cluster from Tg(sox17:gfp) injected embryos were dissected under fluoresence in DMEM/F12 and flash frozen in 2µl of medium in liquid nitrogen. Total RNA were extracted and purified using SMART-Seq®  v4 Ultra® Low Input RNA Kit for Sequencing from clontech. Purified RNA samples were then reverse transcribed into cDNA and amplified. Libraries are constructed using NEB DNA Ultra library construction kit, with standard TruSeq-type adapters. Libraries size and concentration were assessed using Agilent 2,100 Bioanalyzer. Libraries were multiplexed and 50bp single-end were sequenced by BGI generating  a minimum of 20millons read per sample. Genome-wide transcriptome were produced from quadruplicate biological replicates.
GEO Accession
GSM3379517