Illumina HiSeq 2000 paired end sequencing; Gene expression of humanised keratin 12 in mouse cornea. A comparison between wild-type, heterozygous and homozygous genotypes.
Gene expression of humanised keratin 12 in mouse cornea. A comparison between wild-type, heterozygous and homozygous genotypes.
Mice were sacrificed by CO2 asphyxiation Full thickness corneas were dissected, snap frozen and stored at -80Â°C RNA from frozen corneas was extracted after douncing and lysis using the RNeasy Plus Minikit (Qiagen) Illumina TruSeqRNA v2 with polyA capture
Experimental Factor: wild type genotype
genotype