GSM1242494: M0_0h_rep1_miRNAseq; Homo sapiens; RNA-Seq
5x106 -2x107 MΦ were harvested and total RNA including small RNAs was isolated. Small RNA libraries were generated from 1 μg total RNA with the TruSeq Small RNA Sample Preparation Kit (Illumina). After successful ligation of 3’ and 5’ adapters to RNA molecules, RNA was reverse-transcribed using SuperScript II reverse transcriptase (Invitrogen). cDNA was amplified by 11 PCR cycles with high-fidelity Phusion Polymerase (Finnzymes). cDNA with the size of miRNAs plus ligated adapters was purified on a pre-cast 6% Tris/Borate/EDTA polyacrylamide gel electrophoresis gel (Invitrogen).
GEO Accession
GSM1242494