GSM3475465: 84_spln_Id3neg_eTR [lib16886]; Mus musculus; RNA-Seq
CD4+ T cells were isolated using CD4 microbreads (Miltenyi) from either peripheral LNs or spleens of 3 littermate Id3-GFP x Foxp3-mRFP mice. Cells were sorted based on viability, CD4, CD44, CD62L, Id3-GFP and Foxp3-mRFP expression on a FACs Aria II (BD Biosciences). 500 cells were sorted directly into SMART-Seq v4 Ultra Low Input RNA Kit (Takara) lysis buffer and protocol followed to produce cDNA. Library construction was performed using a modified protocol of the NexteraXT DNA sample preparation kit (Illumina).
GEO Accession
GSM3475465