GSM1246252: Abnormal_cloned_group - MeDIP-Seq; Sus scrofa; MeDIP-Seq
DNA of each collected tissue was extracted using using the phenol-chloroform method DNA was sonicated to gain fragments in the range 100–500 bp, dAs were added to the 3' ends of the extracted DNA. The adaptors were then ligated to the two ends of each fragment. The detailed steps can be referenced from the protocol of the Paired-End DNA Sample Prep kit (Illumina). The double-stranded DNA was denatured, and then an antibody that recognizes 5-methylcytosine (5-mc) was used to immunoprecipitate the DNA fragments containing regions of methylated-CpGs (Magnetic Methylated DNA Immunoprecipitation kit; Diagenode). After PCR amplification of the enriched fragments, we selected the 220–320 bp fragments using a Gel Extraction kit (28706; Qiagen). The retracted DNA fragments were quantified using an Agilent 2100 Analyzer.
GEO Accession
GSM1246252