GSM1245211: Normal Homogenate RNA-Seq A; Mus musculus; RNA-Seq
Tumor and normal tissues were homogenized using a glass dounce homogenizer. Lysates were clarified and one half used for poly(A)+ RNA extraction and the other to generate and isolate ribosome-protected RNA fragments. For RNA-Seq libraries: Standard RNA-Seq library preparation. For ribosome profiling libraries: Ribosome footpring libraries were prepared by ligating a 3' adapter, reverse transcribing, and circularizing. The circles were then used for PCR amplification of the footprints.
GEO Accession
GSM1245211