RNA-seq of S. cerevisiae: Cd-exposed cells, transformed with pFL61
The mRNA was sequenced by Illumina technology with Hiseq 2000, raw reads of 50 NTs were firstly assessed for their quality using FastQC and then mapped on S. cerevisiae S288C genome with Burrows-Wheeler Aligner (Li and Durbin 2009). Resulting BAM files were processed using SAMtools (Li et al. 2009). Only reads with phred score greater than 15 were considered in the analysis. The genome sequence and annotation files were obtained from SGD database (http://www.yeastgenome.org).