GSM3141942: CT_stomach_RNASeq_Rep2; Mus musculus; RNA-Seq
Control and Sufu and Spop double knockout E17.5 stomachs were removed, and their epithelial and mesenchymal tissues were separated using EDTA. The mesenchymal part was kept at -20°C prior to RNA extraction with RNeasy kit (Qiagen). The integrity of the samples was checked on Agilent Bioanalyzer, and the samples with a RIN>8.5 were used. 1 to 2µg of RNA were used to prepare the PolyA RNA library. Libraries were prepared according to manfacturer's instructions for the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® kit.
GEO Accession
GSM3141942