GSM3598094: dy2J_1; Mus musculus; RNA-Seq
Quadriceps muscle tissues were isolated from mice and immediately immersed in RNAlater RNA Stabilization Reagent (Qiagen, Valencia, CA), frozen in liquid nitrogen and stored at -80°C. Total RNA was extracted using the miRNeasy mini kit – cat: 217004 (QIAGEN Inc., Valencia, CA) according to manufacturer protocol. The resulting samples were then assessed with a spectrophotometer to estimate their total RNA concentration (NanoDrop 2000c, Thermo Fisher Scientific Waltham, MA, USA) and by the Agilent 2200 TapeStation system (Agilent Technologies, Santa Clara, CA, USA) for quality control. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM3598094