GSM4548769: stimulation_il17_rep2; Mus musculus; RNA-Seq
Total RNA was extracted from the cells using RNeasy Mini Kit (Qiagen) with on column DNAse treatment exactly according the manufacturers protocol. The quality and quantity of isolated RNA was evaluated using Nanodrop and TapeStation 2200 (Agilent Technologies). The ensuing RNA sequencing analysis was based on three independent experiments. For each sample, 1 ug of total RNA (RIN >7.0, rRNA ratio (28S:18S rRNA) >1.0) was sent to Macrogen Inc. for library preparation and sequencing. Briefly, first TruSeq RNA stranded library was generated and then sequencing was performed on the Illumina NovaSeq6000 with 151bp paired-ends configuration, with about 30M reads per sample.
GEO Accession
GSM4548769