GSM1239829: HT; Crassostrea hongkongensis; RNA-Seq
Gills were removed and kept in RNA store. The total RNA was harvested using TRIzol reagent. Poly-A RNA was extracted from each total RNA sample using MicroPoly(A)PuristTM Kit (Part# AM1919M) according to manufacturer’s instructions and pooled for cDNA synthesis. cDNA samples were prepared following the protocol from Ng et al., Nat Methods, 2009. Briefly, first-strand cDNA was synthesized using SuperScript II reverse transcriptase (Cat#18064-014) with Gsul-oligo-dT primer. The second-strand cDNA was synthesized using Ex Taq polymerase (Cat# RR001A). Construct cDNA library. Methods is as followed: the synthetic cDNA was mode to 300-500 bp by a UTR200 sonication device, The fragments were amplified with TruSeq PE cluster kit v3-cBot-HS (Cat# PE-401-3001) and used to construct libraries with TruSeqTM DNA sample prep kit-set A (Cat#FC-121-1001) according to the manufacturer’s instructions.
GEO Accession
GSM1239829