GSM2810112: Esophagitis 5; Homo sapiens; RNA-Seq
Exosomes were isolated from plasma using the Ribo-Exosome-Isolation-Reagent-Kit C10110. Total RNA was isolated from exosome using the Trizol (invitrogen) reagents. cDNA libraries were prepared using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina (ENB, USA). The products were then purified with Agencourt Ampure XP beads (Beckman Coulter, USA) and library quality was assessed using the Agilent 2200 TapeStation system (Agilent Technologies, USA) and then diluted to 10 pM for cluster generation in situ on the HiSeq3000 pair-end flow cell. The clustering of the index-coded samples was performed using the HiSeq Rapid PE Cluster Kit V2 (Illumina). After cluster generation, the libraries were sequenced (2×150 bp) on HiSeq 3000 platform.
GEO Accession
GSM2810112