GSM3834041: KP2; Mus musculus; RNA-Seq
Total RNA was extracted using the RNAeasy Plus mini Kit (Qiagen, Valencia, CA). Quality of RNA  samples was determined using an Agilent Bioanalyzer and only samples with  RINs >9.0 were used for genomic sequencing analysis. Sequencing was carried out  at Mayo Clinic Advanced Genomic Technology Center at Rochester, MN. RNA libraries were prepared using 120 ng of total RNA according to the manufacturer's instructions for the TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego, CA).  The concentration and size distribution of the completed libraries was determined using an Agilent Bioanalyzer DNA 1000 chip (Santa Clara, CA) and Qubit fluorometry (Invitrogen, Carlsbad, CA).   Libraries were sequenced at 50-80 million read pairs per sample following Illumina's standard protocol using the Illumina cBot and Paired end cluster kit version 3. The flow cells were sequenced as 101 X 2 paired end reads on an Illumina HiSeq 2000 using TruSeq SBS sequencing kit version 3 and HCS 2.2.58 data collection software. Base-calling is performed using Illumina's RTA version 1.18.64.
GEO Accession
GSM3834041