Transcriptomic Responses to Estrogen Exposure in Zhikong Scallop Chlamys Farreri
The testis and digestive gland mRNA of male Zhikong scallop Chlamys Farreri of 10d estradiol (17β-E2)exposure (> 98% purity): 0, 50 and 500 ng/L were extracted respectively. Each sample (about 10 μg cDNA each) marked with different barcoded adaptors were located on a 454-sequencing plate and then sequenced on Roche/454 GS-FLX Titanium instrument at the Chinese Academy of Agricultural Sciences following manufacturer’s.