GSM3597926: A3_24h_IL21; Homo sapiens; RNA-Seq
RNA from unstimulated or stimulated IE-CTLs was isolated with the RNeasy plus mini kit (Qiagen). RNA concentration and quality were measured using the Nanodrop 1000 Spectrophotometer (Thermo Scientific) and the high-sensitivity RNA analysis kit (Experion, software version 3.0, Bio-Rad). RNA-seq libraries were prepared from 1ug of total RNA using the Quant seq 3' kit (Lexogen).  The libraries were sequenced on the Nextseq500 (Illumina) yielding at least fifteen million sequence reads per sample.
GEO Accession
GSM3597926