RNA-seq of rice root non-colonised by nitrogen fixing bacterium Herbaspirillum seropedicae SmR1
Total RNA was extracted from roots of five rice plants using RNAqueos kit (Ambion). Total RNA (7 to 10 g) was depleted of ribosomal RNA by treatment with a RiboMinus Plant Kit for RNA-Seq (Invitrogen). Whole Transcriptome Analysis RNA Kits (Life Technologies) were used on 500 ng purified RNA to construct the libraries and sequencing was performed in a SOLiD4 (Life Technologies) sequencer.