zebrafish ZF4 cell after PBS treatment at 24h
Library construction: Illumina HiSeq 2000 protocol. Experimental design: MMS (Sigma, USA) was dissolved in phosphate-buffered saline (PBS) just before use. Zebrafish ZF4 cells (ATCC CRL-2050) derived from 1-day-old zebrafish embryos were routinely maintained in a humidified chamber (28C, 5% CO2) in DMEM/F-12 medium (GIBICO, USA), with 10% (v/v) fetal bovine serum (GIBICO, USA), 100 U/ml penicillin and 100 mg/ml streptomycin (GIBICO, USA). When confluence reached 70–80 %, cells were treated with 639.16μM MMS or an equal volume of PBS (control group) and incubated for 24 h. Cells were washed 3 times with cold PBS and detached from the culture plate using trypsin (GIBICO, USA) for further experiments.