Ammonia unamended soil from 56-days aerobic dynamic: day 56
This paired-end SRA file contains the V1-V3 16S rRNA raw sequences as a multiplexed library from the 18 ammonia unamended soil samples for Day 56 of the 56-days aerobic dynamic. The paired-end fastq files must be fed into the split_libraries_fastq.py script to demultiplex each Sample by its 16-bp unique barcode. The mapping file to be used is the following; where '$' must be changed to tabulation ('\t') and '@' to a new line ('\n'): SampleID$BarcodeSequence$LinkerPrimerSequence$ReversePrimer$soil_use$dinamic$Practice$Fertilizer$Day$Treatment$Pract.Day$Pract.Fertil.Day$Day.Fertilizer$Soil$OH$Description@DNA271$TAAGGCGATAGATCGC$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$unamended$CT$0$56$CT0$CT.56$CT.0.56$D56.0$111$1$Soil.CTRL.127@DNA272$TAAGGCGACTCTCTAT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$unamended$CT$0$56$CT0$CT.56$CT.0.56$D56.0$211$2$Soil.CTRL.128@DNA273$TAAGGCGATATCCTCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$unamended$CT$0$56$CT0$CT.56$CT.0.56$D56.0$311$3$Soil.CTRL.129@DNA274$TAAGGCGAAGAGTAGA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$unamended$CT$300$56$CT300$CT.56$CT.300.56$D56.300$115$4$Soil.CTRL.130@DNA275$TAAGGCGAGTAAGGAG$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$unamended$CT$300$56$CT300$CT.56$CT.300.56$D56.300$215$5$Soil.CTRL.131@DNA276$TAAGGCGAACTGCATA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conventional$unamended$CT$300$56$CT300$CT.56$CT.300.56$D56.300$315$6$Soil.CTRL.132@DNA277$TAAGGCGAAAGGAGTA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBB$0$56$PBB0$PBB.56$PBB.0.56$D56.0$121$7$Soil.CTRL.133@DNA278$TAAGGCGACTAAGCCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBB$0$56$PBB0$PBB.56$PBB.0.56$D56.0$221$8$Soil.CTRL.134@DNA279$CGTACTAGTAGATCGC$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBB$0$56$PBB0$PBB.56$PBB.0.56$D56.0$321$9$Soil.CTRL.135@DNA280$CGTACTAGCTCTCTAT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBB$300$56$PBB300$PBB.56$PBB.300.56$D56.300$125$10$Soil.CTRL.136@DNA281$CGTACTAGTATCCTCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBB$300$56$PBB300$PBB.56$PBB.300.56$D56.300$225$11$Soil.CTRL.137@DNA282$CGTACTAGAGAGTAGA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBB$300$56$PBB300$PBB.56$PBB.300.56$D56.300$325$12$Soil.CTRL.138@DNA283$CGTACTAGGTAAGGAG$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBC$0$56$PBC0$PBC.56$PBC.0.56$D56.0$151$13$Soil.CTRL.139@DNA284$CGTACTAGACTGCATA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBC$0$56$PBC0$PBC.56$PBC.0.56$D56.0$251$14$Soil.CTRL.140@DNA285$CGTACTAGAAGGAGTA$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBC$0$56$PBC0$PBC.56$PBC.0.56$D56.0$351$15$Soil.CTRL.141@DNA286$CGTACTAGCTAAGCCT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBC$300$56$PBC300$PBC.56$PBC.300.56$D56.300$155$16$Soil.CTRL.142@DNA287$AGGCAGAATAGATCGC$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBC$300$56$PBC300$PBC.56$PBC.300.56$D56.300$255$17$Soil.CTRL.143@DNA288$AGGCAGAACTCTCTAT$CCTACGGGNGGCWGCAG$GACTACHVGGGTATCTAATCC$Conservation$unamended$PBC$300$56$PBC300$PBC.56$PBC.300.56$D56.300$355$18$Soil.CTRL.144