pyrosequencing of 16S RNA gene of soil DNA
For each sample, 16S rRNA genes were amplified using the following primer set, 27f 5'-AGAGTTTGATCMTGGCTC-3' and EUB518 5'- ATTACCGCGGCTGCTGG -3', targeting the V1-V3 hypervariable 16S rRNA region. The reverse primer included the sequence of the Titanium A adaptor (5′-CCATCTCATCCCTGCGTGTCTCCGACTCAG-3′) and a barcode sequence.