Plate2-B03
DNA was isolated from cecal contents and fecal pellets using the QIAamp DNA stool mini kit (Qiagen). Vigorous homogenization of the samples was performed with 300 mg of 1.0 mm zirconia beads (BioSpec, Bartlesville, OK, USA) using a MiniBeadBeater (BioSpec, Bartlesville, OK, USA). After extraction, DNA was eluted in 200_l Elution Buffer and stored at _20C. 16S libraries were pooled and sequenced according to the protocol developed by Kozich et al., and the detailed version of the specific protol used is provided in the supplemental text of the manuscript associated with this SRA submission. Briefly, the V4 region of the 16S rRNA gene was amplified and sequenced using the MiSeq Reagent Kit v2 that produces up to 25 million reads of 2 x 250 bp per run at the Genomics Core Facility at UVA.  Reads were assigned to samples using Illumina BaseSpace demultiplexing.