Illumina HiSeq 3000 paired end sequencing; Transcriptome profiling of short-term response to chilling stress in resistant and susceptible rice seedlings
Transcriptome profiling of short-term response to chilling stress in resistant and susceptible rice seedlings
Rice seeds were de-hulled, sterilized with 5% (v/v) hypochlorite and washed several times with sterile water. Seeds were maintained in 1%-agar plate ½ MS medium in the dark at 30°C for 2 days. Seedlings were grown in a growth cabinet under a 14 h-light photoperiod, PAR 50 µmol m-2 s-1 provided by fluorescent lamps with temperature of 28/10°C day/night. Total RNA was extracted from shoots using Spectrum™ Plant Total RNA Kit (Sigma Aldrich, USA) and DNA was removed using the Turbo DNA-free™ Kit (Ambion by Life Technology, USA). lllumina TruSEQ RNA-Seq library construction protocol, not stranded
Experimental Factor: cultivar
Volano
Experimental Factor: environmental stress
cold temperature regimen
Experimental Factor: time
10