Illumina HiSeq 4000 sequencing; RNA-seq of WT, Mbd3-/- and rescue hiPSC during neuroectodermal differentiation and of WT and Mbd3-/- in mEpiSC during neuroectodermal differentiation
RNA-seq of WT, Mbd3-/- and rescue hiPSC during neuroectodermal differentiation and of WT and Mbd3-/- in mEpiSC during neuroectodermal differentiation
Cells were grown in self-renewing or differentiation conditions for specific times and harvested directly in TRI Reagent. RNA made from samples in TRI Reagent using Direct-zol RNA Miniprep Kit (Zmyo Research) according to manufacturers protocol Library Selection: Poly(A)+, Library Strand: First Strand
Experimental Factor: organism
Homo sapiens
Experimental Factor: genotype
Rescue
Experimental Factor: time
3