GSM1922670: AOF1_WT_3; Mus musculus; Bisulfite-Seq
PBAT To obtain MII oocytes, 6-8-week-old mice were injected intraperitoneally with 5 IU of pregnant mare’s serum gonadotrophin (PMSG, Sigma) and, 48 hours later, with 5 IU of human chorionic gonadotrophin (hCG, Sigma). Superovulated mice were euthanized 16 hours after hCG injection, and oocytes collected from the oviducts and released into a hyaluronidase/M2 solution for removal of cumulus cells. Genome-wide methylation maps in KDM1A- and KDM1B-deficient MII oocytes and controls were generated using the PBAT method. For each genotype, triplicate PBAT libraries were generated, with ~100 MII oocytes per library.
GEO Accession
GSM1922670