Strand-specific mRNA for Chaetosphaeria tulasneorum (NRRL18230)
- Liquid media 24°C for 4 days 200 rpm - No treatment. - Extraction and clean-up of RNA: phenol/chloroform extraction, followed by clean-up using the RNeasy plant mini kit (Qiagen), and DNase treatment using the Turbo DNA free kit (Ambion). - TruSeq stranded total RNA