GSM4547622: Trec_pooled; Mus musculus; RNA-Seq
Cells were loaded onto the Chromium Controller (10x Genomics) for a target recovery of 5,000 single cells. The scRNAseq libraries were generated using Chromium Single Cell 3′ Library and Gel Bead Kit v2 (10x Genomics) according to the manufacturer's protocol. Briefly, emulsions were created and, after reverse transcription, gel-beads in emulsion were disrupted. Barcoded complementary DNA was isolated and amplified by PCR (12 cycles). Following fragmentation, end repair and A-tailing, sample indexes were added during index PCR (8 cycles). Samples were pooled and quantified using a KAPA Library Quantification Kit (Kapa Biosystems) prior to sequencing
GEO Accession
GSM4547622