GSM1923373: Gly_cytoRP_15; Saccharomyces cerevisiae; RNA-Seq
sample processing: RNaseI treatment, 80S monosome isolation on 10-50% sucrose gradient, RNA isolation by phenol/chloroform extraction,  gel purification of 27-33 nt RNA fragments Gel-purified RNA was treated with PNK and ligated to a pre-adenylated adapter using T4 RNA ligase 2, K227Q, truncated. Superscript III was used for reverse transcription and circ ligase for circularization. Illumina adapters were introduced by 10-14 cycles of PCR.
GEO Accession
GSM1923373