GSM2991026: WT; Mus musculus; RNA-Seq
Cortices were harvested at embryonic day 14.5, and immediately frozen at -80 ℃. After genotyping, RNA was isolated with Trizol reagent. Illumina TruSeq RNA Sample Prep Kit was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols. After digesting the total RNA of the sample and digesting the DNA with DNaseI, the mRNA in total RNA is purified with Oligo d (T) magnetic beads, and the resulting mRNA is fragmented by adding an appropriate amount of an interrupting reagent under high temperature conditions. As a template, cDNA was synthesized, purified by magnetic beads, the end of the repair, 3' end plus base A, plus sequencing linker, PCR amplification, thus completing the entire library preparation.
GEO Accession
GSM2991026