GSM1247445: WT +GLU RNA-seq 1; Saccharomyces cerevisiae; RNA-Seq
frozen cell pellets were thawed in lysis buffer and lysis was performed with glass beads as described in the text. Extracts were clarified by a 20' spin at 11,500 rpm in an SS-34 rotor Footprints: 50 OD units of extract were treated with 50U of RNAse1 and subsequently purified from the monosome region of a sucrose gradient. RNA was extracted by hot acid phenol. Total RNA: RNA was extracted from 50 OD of extract by SDS and hot acid phenol, and poly(A) RNA was purified on DynaBeads. RNA was fragmented at 95C with zinc chloride
GEO Accession
GSM1247445