Gene networks controlling drought traits in Gossypium herbaceum L.
Total RNA (3 µg) was reverse transcribed using a T7-Oligo (dT) Promoter Primer in the first-strand cDNA synthesis         reaction.Following RNase H-mediated second-strand cDNA synthesis, the double-stranded cDNA was for further enrichment         double-stranded cDNA served as a template in the subsequent in vitro transcription (IVT) reaction.The IVT reaction was carried out         in the presence of T7 RNA Polymerase. cRNA(3µg) was then reverse transcribed in the first-strand cDNA synthesis step of using random         hexamer primer, followed RNase H-mediated second strand cDNA synthesis in replicates, the samples were pooled and purified by column         (QIAquick PCR purification kit), sample was used for sequencing.