DNA-Seq of C. Albicans: JRCT1
Genomic DNA was prepared by glass bead beating followed by phenol-chloroform extraction. The DNA concentration was determined by use of Qubit HS DNA reagent (Invitrogen). The DNA quality was determined by gel imaging and absorbance ratios (260/280 and 260/230 ratios of 1.8 to 2.2). The genomic DNA was fragmented by sonication. A VAHTS Nano DNA library prep kit for Illumina (Vazyme) was used for library preparation, including blunt ending, dA tail addition, ligation, and library amplification.