GSM4273012: coordinate reads for PBMC mRNA-seq; synthetic construct; OTHER
Cell-containing drops were merged with lysis buffer drops containing coordinate oligos and barcoded poly dT beads within microwells. Beads were then processed using the Drop-Seq protocol, using the modified CITE-seq/Cell hashing protocol to separate coordinate oligo reads from mRNA reads. The coordinate library directly had sequencing adapters added with PCR. The mRNA library underwent tagmentation and sequencing adapter addition using the Illumina Nextera XT sample preparation kit. A custom Read 1 primer used in Drop-Seq was used during the sequencing of both libraries. Drop-Seq; CITE-Seq/Cell hashing
GEO Accession
GSM4273012