GSM2990901: 1256_48hr_2; Saccharomyces cerevisiae; RNA-Seq
Cells were grown in 50mL volumes to the indicated time point. 3mL of cells was taken and spun down for RNA acid-phenol extraction followed by standard etOH precipitation. A minimum of 5 ug of RNA was used as input for NEBNext® Poly-A enrichment and Ultra Directional II cDNA library construction. Poly-A enrichment coupled with directional cDNA dUTP second strand synthesis.
GEO Accession
GSM2990901