RNA-Seq of Bidens pilosa: wounded leaf
Total RNA was extracted by using the TriZol reagent (Invitrogen, Carlsbad, California, U.S.A.) according to the manufacturers instructions. To avoid DNA contamination, total RNA samples were subjected to DNase treatment using RQ1 RNase-Free DNase (Promega) and purified by 2.5 M LiCl precipitation. The quality of RNA samples was analyzed by a bioanalyzer, and RNA-seq libraries were prepared using the strand specific TruSeq RNA library preparation kits. All procedures followed the protocols provided by the manufacturer.