GSM2219084: 2dA(T1); Aspergillus niger; RNA-Seq
Frozen conidia in Saline/tween were homogenized in a stainless steel grinding jar using a TissueLyser II (Qiagen, www.qiagen.com). RNA was isolated using a combination of the maxi RNeasy kit (Qiagen RNeasy; www.qiagen.com), the Master Pure Yeast RNA purification kit (Epicentre, www.epibio.com) and the mini RNeasy kit (Qiagen RNeasy; www.qiagen.com) (van Leeuwen et al., 2012). RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM2219084