Profiling miRNAs in mouse embryonic stem cells with mutations in the Dgcr8 locus
Profiling miRNAs in mouse embryonic stem cells with mutations in the Dgcr8 locus
Cells were  lysed with Trizol while sub-confluent. Large RNA was removed from the small RNA fraction using the RNeasy Mini Kit (Qiagen), and small RNAs were collected by isopropanol precipitation and quantitated on an Agilent Technologies 2100.
Experimental Factor: Genotype
Dgcr8 tm1,gt1/+