CDC50A as a Novel Marker for Epithelial Ovarian Cancer Stem Cells
Tumor tissues were removed by cytoreductive surgery with consensus from 3 patients with invasive EOC enrolled into Peking Union Medical College Hospital from March to June of 2014 (Supplementary Table 8). CDC50A+Lin- and CDC50A-Lin- cells were sorted from these tumor samples by Moﬂo Beckman Coulter (Fullerton, CA). Total RNA was extracted from CDC50A+Lin- and CDC50A-Lin- cells respectively using RNeasy Mini Kit (Qiagen). To construct cDNA libraries using the TruSeq RNA Sample Preparation Kit v2 (Illumina), polyadenylated RNA was purified from 1 μg of total RNA using magnetic beads and fragmented according to the manufacturer’s instructions. Following ligation of the paired-end adapter, the approximately 200-bp fracments were amplified with 15 cycles of PCR. RNA sequencing was performed with the Illumina HiSeq 2500 platform.