RNA-seq analysis of nile tilapia
We treated XX Nile tilapia (Oreochromis niloticus) by RU486 from 5 dah (days after hatching) to 4 mah (months after hatching).  Subsequently, high throughput RNA-seq of gonads from control XX, XY and RU486 treated XX fish were carried out and lncRNAs expression profiles were  extensively analyzed. Then, we conducted comparative gonadal transcriptome analysis, and investigated histological and transcriptional differences using 4 mah tilapia.