16S rDNA fragment gene of microbial community from  citrus plant samples infected with Candidatus liberibacter asiaticus
The V1-V3 variable region of the 16S rDNA gene was amplified using standard PCR from the extracted gDNA with primers 27F (GAGTTTGATCMTGGCTCAG) and 515R (TTACCGCGGCTGCTGGCAC). After this PCR amplification, the amplicons were purified (Qiagen PCR purification kit) and quantified (Qubit). Barcoded amplicons were pooled and sequenced using a PacBio-RSII sequencer (WSU genomics core, USA).