GSM1603270: Nur77(LIF)_ChIP_Seq; Mus musculus; ChIP-Seq
mESC or neuronal cells on 100mm plates were cross-linked with 1% formaldehyde for 10 min while shaking at room temperature and rinsed three times with cold phosphate-buffered saline. Cells from five plates were pooled and harvested in phosphate-buffered saline with protease inhibitors by 10 min centrifugation at 4C. All ChIP assays were performed with 112 μg of chromatin and 3 μg of FGFR1, RXR, Nur77 or H3.3 antibodies using the Invitrogen MAGnify ChIP Kit according to manufacturer's instructions with slight modifications. Genomic DNA was precipitated with ethanol, treated with RNase A and proteinase K, and purified using the Qiagen PCR purification kit Chromatin was further prepared using the Tru-seq ChIP Sample Preparation Kit and purified library DNA was captured on an Illumina flowcell for cluster generation and sequenced on an Illumina Hisequation 2000, following the manufacturer's protocols.
GEO Accession
GSM1603270