16S rRNA sequencing of Artic Lakes in West Greenland
The 16S rRNA gene V3/V4 was amplifed with nested primers modified to add Illumina adapter overhang sequences. After initial amplification, library size was verified on an Agilent DNA1000 chip and cleaned using AxyPrep Mag beads. Illumina dual indexes and sequencing adapters were then added using indexed primers. Final amplicons were cleaned, quality assessed, stadardized to 2 , and pooled.