Whole genome sequencing of Oryza granulata
Fresh and healthy leaves were harvested and immediately frozen in liquid nitrogen, followed by the preservation at -80C in the laboratory prior to DNA extraction. High-quality genomic DNA was extracted from leaves using a modified CTAB method. RNase A was used to remove RNA contamination. The quality and quantity of the extracted DNA were examined using a NanoDrop 2000 spectrophotometer (NanoDrop Technologies, Wilmington, DE) and electrophoresis on a 0.8% agarose gel, respectively.