GSM2817844: alpha7E260A:G Smoked Male; Mus musculus; RNA-Seq
Lungs were cleared of BALF content, the trachea removed and the more distal lung lobe tissue enriched by dissection. The interstitial cells were collected and reacted with anti-CD45 antibody coupled to magnetic beads before depletion of the CD45+ cells using an autoMACS cell separator .The purity was (>95% epCAM+, <1% CD31+ endothelial cells) and viability (<5% 7-AAD (viability stain) Standard methods to prepare poly-adenylated strand-specific RNA-Seq libraries using Illumina TruSeq stranded mRNA preparation kits.
GEO Accession
GSM2817844