GSM1258444: 36:11 Eed-/- DOX; Mus musculus; ChIP-Seq
Lysates were clarified from sonicated nuclei and JARID2-DNA complexes were isolated with JARID2 antibody (Li et al., 2010) ChIP-seq libraries were prepared according to Illumina protocol and sequenced using a HiSeq machine. Single-end sequencing was carried out to obtain about 50 million (M), 100bp long reads per sample. Read alignment with Bowtie2-2.1.0 (Langmead and Salzberg, 2012; Langmead et al., 2009) with standard parameters and one mismatch in the seed sequence and consecutive duplicate filtering resulted in at least 35M reads per sample. Only reads with mapping quality Qphred20 were kept for the enrichment analysis.
GEO Accession
GSM1258444