GSM3143778: RNA-seq_epe1KO_rep2; Schizosaccharomyces pombe; RNA-Seq
RNA was isolated by bead beating with 0.5mm zerconia bead in Trizol reagent (Thermo Fisher) and purifying by chloroform extraction. 1g of total RNA was used to isolate mRNA using PolyATtract® Systems III and IV (Promega). Samples were Dnase treated using the Zymo RNA Clean & Concentrator Kit 5® (Zymo Research). Sequencing libraries were constructed using the NEBNext Ultra Directional RNA library Prep Kit for Illumina (New England Biolab).
GEO Accession
GSM3143778