western pool
Genomic DNA was isolated from muscle, fin or blood samples using the peqGOLD Tissue DNA Mini Kit (Peqlab Biotechnologie) following manufacturer's instructions. The quantity and quality of DNA was measured with Qubit® 2.0 Fluorometer and 0.8% agarose gel electrophoresis. The DNA samples with concentrations ≥ 50 ng/µl, 260/280 ratios of 1.8-2.0 and clear high molecular weight bands on the gel were used for genome sequencing.