Illumina HiSeq 2500 sequencing; Dynamical Modeling of Secondary Metabolism and Metabolic Switches in Streptomyces xiamenensis 318
Dynamical Modeling of Secondary Metabolism and Metabolic Switches in Streptomyces xiamenensis 318
For total RNA extraction, cells in the early and late stationary phase at 16h, 24h, 36h and 72h were rapidly cooled using chilled ethanol and harvested by centrifugation (12,000 rpm, 5 min, 4 °C). Total RNA was extracted using RNeasy Mini Kit (Cat#74106, Qiagen) following the manufacturer's instructions and checked for a RIN number to inspect RNA integrity by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US). Qualified total RNA was further purified by RNeasy micro kit (Cat#74004, QIAGEN, GmBH, Germany) and RNase-Free DNase Set (Cat#79254, QIAGEN, GmBH, Germany). The following procedures are mRNA enrichment by rRNA depletion, mRNA fragmentation, first and second cDNA strand synthesis, and library construction.
Experimental Factor: sampling time point
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