16S rRNA of Homo sapiens: preschool child nasal swab: Sample 181
V1-3 region of the 16S ribosomal gene was PCR amplified. The Roche Expand High Fidelity enzyme was used to amplify the target region using an Eppendorf thermal cycler. Sequencing libraries were generated using the Nextera XT Index kit and amplicons were sequenced on the Illumina MiSeq sequencer.