ZR-75-1 ChIP seq INPUT channel
Plated at 12,000,000 cells/plate on 150mm diameter plates for 72h in phenol red free RPMI 1640 with 10% Charcoal stripped FCS. After fixation of the cells, lysates were clarified from sonicated cells, and then INPUT DNA isolated from the clarified lysates. INPUT consists of samples pooled across experiments. Libraries were prepared according to Illumina's instructions.