GSM2652244: BMDM_R0A; Mus musculus; RNA-Seq
cell lysate extraction for ribosome profiling analysis and RNA isolation were performed in accordance with the protocols detailed in Ingolia et al., 2012 (PMCID: PMC3535016); The CLIP-seq was performed in accordance with the protocols described in Moore et al., 2014 (PMCID: PMC4156013 ). The anti-V5 antibody in the CLIP-seq was from Bethyl Lab (Cat# A190-120A, note: the vendor did not provide the lot number) The ribosome profiling libraries were constructed in accordance with the protocol described in Ingolia et al., 2012 (PMCID: PMC3535016); the RNA-seq libraries were constructed on the rRNA-depleted (ribo-zero) total RNAs using the TRUE-seq kit (illumnia); the CLIP-seq libraries were constructed in accordance with the protocols detailed in Moore et al., 2014 (PMCID: PMC4156013 )
GEO Accession
GSM2652244