RNA-seq of Nasonia vitripennis x Nasonia giraulti F1 hybrid: 48-hour adult female, whole body
Total RNA samples were extracted from a pool of 4 adult female whole-body samples 48 hours after eclosion using a Qiagen RNeasy Plus Mini kit (Qiagen, CA). Nasonia vitripennis (strain AsymCX) and Nasonia giraulti (strain RV2Xu) were used to produce either pure-species or hybrid samples. Three independent biological replicate batch samples were prepared for each species and each reciprocal hybrid. RNA-Seq libraries were prepared with 2ug total RNA input using the Illumina stranded mRNA library prep kit.