cDNA from eyestalk was sequenced using an Illumina platform with the specific scope to identify and characterize clock genes (i.e. circadian clock). Sample: darkness
Lobsters were maintained under 12-12 light-darkness conditions. Eyestalks were dissected at the middle of the darkness period. The cuticle and the retina were rapidly eliminated and the remaining tissue immediately transferred to RNA-later tissue collection (Invitrogen Inc.) and stored at -80°. Total RNA isolation was performed using the column-based RNeasy® Mini Kit (Qiagen Inc.) following manufacturer instructions.  The mRNA fraction of the total RNA was converted into a library of template molecules suitable for subsequent cluster generation using the reagents provided in the Illumina TruSeq RNA Sample Preparation Kit.