P_splendens_RNAseq
The whole RNA was extracted using mirVana RNA isolation kit as per the manufacturers description. Total RNA extracted from the two samples i.e Pythium splendens mycelium & Pythium splendens from infected Piper nigrum by RNA extraction method. The quality of RNA from the samples was measured using Agilent 2100 Bioanalyzer and the quantity was measured by using Nanodrop. The samples having RNA integrity number (RIN) passing value of 8.0 was further used for sequencing.