GSM4276560: AR ChIP-exo; Homo sapiens; ChIP-Seq
Biological replicates of LNCaP AR-V7 cells treated with 10uM R1881 or 20ng/mL for 24 hours were formaldehyde fixed and and pooled together.  Nuclear extracts were prepared in a water bath sonicator and DNA-protein complexes were isolated with AR antibody and Protein A/G Magnetic Agarose. ChIP-exo libraries were prepared using ChIP-exo Kit from ActiveMotif following manufacturer's instructions. Libraries were sequenced on Illumina HiSeq 2500 following the manufacturer's protocols. Libraries were constructed using ChIP-exo library preparation kit (ActiveMotif, USA) following the manufacturer's instructions. cDNA libraries were sequenced on the Illumina HiSeq 2500 platform by ActiveMotif, USA.
GEO Accession
GSM4276560