GSM2057911: DF78_7dpi-BR1; Brassica napus; Leptosphaeria maculans; RNA-Seq
RNA was collected from three biological replicates of infected and mock inoculated B. napus cotyledons at 0, 3, 7, and 11 dpi. Total RNA was isolated by using PureLink® Plant RNA Reagent (Ambion) and treated with TURBO DNA-free™ Kit (Ambion) according to the manufacturer’s instructions. RNA quality and integrity was measured using the 2100 Bioanalyzer (Agilent Technologies) with the Agilent 2100 PicoChip. RNA-sequencing libraries were prepared according to alternative HTR protocol (C2) established by R. Kumar et al. (2012) with the exception of library PCR enrichment that was carried out using 11 PCR cycles. RNA sequencing libraries were validated using the Agilent Bioanalyzer DNA chips (Agilent Technologies) and quantified using the Quant-iT dsDNA Assay kit (ThermoFisher Scientific).
GEO Accession
GSM2057911