GSM4218372: ddcc_rep2; Arabidopsis thaliana; RNA-Seq
Total RNA was extracted from leaves tissue using RNeasy plant mini kit (Qiagen) according to manufacturer's instructions The polyA fraction (mRNA) was purified from 500 ng of total input RNA, followed by fragmentation and the generation of double-stranded cDNA. Then, Agencourt Ampure XP beads cleanup (Beckman Coulter), end repair, A base addition, adapter ligation and PCR amplification steps were performed. Libraries were quantified by Qubit (Thermo fisher scientific) and TapeStation (Agilent).
GEO Accession
GSM4218372