GSM2651907: OrR3; Drosophila melanogaster; RIP-Seq
Embryos were dechlorionated with hypochloride, frozen in liquid nitrogen. RNA and protein complexes were isolated by antibody or GFP binder. RNA were further extracted with phenol/chloroform/isoamylalcohol and precipitated. The RNA profile were analyzed by next generation sequencing. Library preparation for RNA-Seq was performed using the TruSeq RNA Sample Preparation Kit (Illumina, Cat. N°RS-122-2002) starting from 500 ng of total RNA according to Illumina's instructions. Accurate quantitaion of cDNA libraries was performed by using the QuantiFluor™ dsDNA System (Promega). The size range of final cDNA libraries was determined applying the DNA 1000 chip on the Bioanalyzer 2100 from Agilent (280 bp). The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the HiSeq2000  following the manufacturer's protocols.
GEO Accession
GSM2651907