GSM1689907: C_difficile_Planktonic_1; Peptoclostridium difficile R20291; RNA-Seq
The RNeasy™ RNA purification kit (Qiagen) was used per the manufacturer’s instructions. Total RNA samples were treated with DNase I (Invitrogen). The level of ribosomal RNA present in total RNA samples was reduced prior to library construction using the Ribo-Zero™ Gram Positive Bacteria rRNA Removal Kit (Epicentre Technologies, Madison, WI). RNA libraries were prepared for sequencing using standard Illumina protocols