RNA-seq of antennae from developing clonal raider ant pupae
Pupae were syncronized by checking prepupae every 4hrs and seperating all newly transition pupae. Pupae were then aged 0-14 days before antennae were dissected and flash frozen. RNA was extracted with the Trizol reagent and Phaselock columns, then cleaned with the QIAGEN RNEasy kit before being sent to the Rockefeller University Genomics Core Facility for library prep and sequencing