GSM2055669: WT 6h Refed; Mus musculus; RNA-Seq
Isolated primary islets from 3 WT and 3 KR mutant mice after 24 hr-fasted or 24 hr-fasted followed by 6 hr-refeeding were combined per genotype and total RNA isolation was performed using RNeasy Mini Kit (QIAGEN). We use poly-A pull-down to enrich mRNAs from total RNA samples (200ng-1ug per sample, RIN>8 required) and proceed on library preparation by using Illumina TruSeq RNA prep kit.
GEO Accession
GSM2055669