targeted gene sequencing of homo sapiens: patient 2603 tumor
The targeted sequencing was performed on the 36 matched tumor and blood DNA samples from the same cohort. Capture of the target regions (exons plus splice junctions) was carried out using a custom-designed NimbleGen SeqCap Target Enrichment kit (Roche) per manufacturer protocols. The DNA libraries were prepared using the SeqCap EZ Choice Library Kit.  Libraries were sequenced using 125 bp paired-end reads on Illumina HiSeq 2500 at the UM DNA Sequencing Core.