Illumina NovaSeq 6000 paired end sequencing; RNA-seq of primary mesenchymal stem cells -derived osteoblasts (differentiated with BMP2) from 3 human donors
RNA-seq of primary mesenchymal stem cells -derived osteoblasts (differentiated with BMP2) from 3 human donors
Primary bone-marrow derived human MSCs isolated from healthy donors were plated in osteogenic media at 15,000 cells/cm2 and the next day stimulated with recombinant human BMP2 in serum-free osteogenic media. Cells were harvested at 72 hours following BMP2 treatment. Total RNA was isolated from differentiating osteoblasts using TRIzol reagent by following manufacturer instructions. Total RNA was depleted of rRNA utilizing the Ribo-Zero rRNA Removal Kit (Illumina). The depleted RNA was then made into RNA-seq libraries using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB).
Experimental Factor: individual
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