GSM2995278: 15#08388 Eedwt/wt (WT); Mus musculus; ChIP-Seq
Cells were collected via FACS using Oct4GFP expression to identify germ cells. Approximately 10,000 germ cells were FACS purified from individual Eedwt/wt (n=4) and Eedhypo/hypo (n=4) embryos and ChIP performed as described [81, 82] with minor alterations. Briefly, cells were cross-linked in 1% Formaldehyde/PBS for 5 min before adding Glycine/PBS at a final concentration of 125mM. Cells were pelleted and washed in PBS and stored -80°C. Libraries were prepared from using the Ovation Ultralow System V2 using Nugen protocol M01379v1. Each library was quantitated using a Qubit instrument and the DNA size profile determined using an Agilent Bioanalyzer. Libraries were finally quantitated by qPCR, pooled in an equimolar ratio and all eight libraries run on one lane of a HiSeq1500 sequencer to obtain 50bp single end reads.
GEO Accession
GSM2995278