GSM1260064: Ter119+ total RNA minus rRNA; Mus musculus; RNA-Seq
E14.5 mouse fetal liver cells were separated into TER119+ and TER119- fractions via magnetic-assisted cell sorting. Total RNA was isolated from these cells using the QIAGEN miRNeasy Kit according to the manufacturer’s instructions. Ribosomal RNA was depleted from 4 ug total RNA using the Ribo-Zero Gold Kit from Epicentre. Strand-specific sequencing libraries were generated following a previously described protocol (PMID: 21943893) from the total RNA (TER119- and TER119+ cells) or from the poly(A)+ and poly(A)- fractions (TER119+ cells). The latter fractions were separated using the Solexa kit (Illumina) according to the manufacturer’s instructions. cDNA fragments of 400-600 bp from these libraries were selected by gel purification and then sequenced on a Illumina HiSeq2000 sequencer.
GEO Accession
GSM1260064