GSM4056892: cDC2_CD5-CD163+CD14-_SLE_R6790; Homo sapiens; RNA-Seq
PBMCs were isolated from healthy subjects and patients with autoimmune disease and cDC2 subsets were defined by FACS. cDNA libraries were prepared from 100 cells using the SMARTSeq v2 protocol (Picelli et al., 2014) with the following modifications: (i) 1 mg/mL BSA Lysis buffer (Ambion Thermo Fisher Scientific, Waltham, MA, USA); (ii) addition of 20 mM TSO; and (iii) 200 pg cDNA with 1/5 reaction of Illumina Nextera XT kit (Illumina, San Diego, CA, USA). The length distribution of the cDNA libraries was monitored using a DNA High Sensitivity Reagent Kit on the Perkin Elmer Labchip (Perkin Elmer, Waltham, MA, USA). All samples were subjected to an indexed paired-end sequencing run of 2x151 cycles on an Illumina HiSeq 4000 system (Illumina, San Diego, CA, USA), with 23- 24 samples/lane.
GEO Accession
GSM4056892