WGS_Allorhizobium_vitis_K306
A single bacterial colony was inoculated into a 5 mL LB broth and grown overnight at 28oC. After centrifuging the cells and discarding the supernatant, genomic DNA was purified from the cells by using a Qiagen DNeasy PowerSoil Kit (Qiagen). The purified DNA was sheared to 10-15kb utilizing needle shearing and then a SMRTbell library was constructed by using the SMRTbell Template Prep kit 1.0 (PacBio, Menlo Park, CA)