GSM4218191: As_3; Mus musculus; RNA-Seq
Lungs were perfused with PBS, collected, and whole lung strained through 70- and 30-µm filters to obtain a single-cell suspension. Dead cells were removed (annexin V EasySep kit, StemCell Technologies), and samples were enriched for CD45+ cells by magnetic separation with CD45 microbeads (Miltenyi). Single-cell suspensions of 6 samples were loaded on a Chromium Single Cell system (10X Genomics) to generate single cell libraries. Libraries were constructed using Single Cell 3' Version 2 chemistry (10X Genomics). Libraries were multiplexed and sequenced on 4 lanes of a Nextseq 500 sequencer (Illumina).
GEO Accession
GSM4218191