GSM3602785: ChIP-MR_replicate-R; Rattus norvegicus; ChIP-Seq
Whole hippocampus were dissected, snap frozen in liquid N2 and stored at -80C until processing. Hippocampi from 2 rats were cross-linked in 1% formaldehyde containing inhibitors and sonicated  to extract chromatin. Chromatin was immunoprecipitated using antibodies against MR (MR H-300, sc11412x, Santa Cruz) or GR (GR H-300, sc8992x, Santa Cruz) and inputs were prepared from original chromatin sample in the absence of antibody. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (WaferGen PrepX Complete ILMN 32i) using an Apollo system for end-repair, A-tailing, and ligation. The adapter-ligated samples were then single-indexed and PCR amplified.
GEO Accession
GSM3602785