GSM4276219: Unamputated; Mus musculus; RNA-Seq
Tissue was manually dissected followed by trypsin and collagenase I enzymatic digestion. Ref blood cells were lysed with ACK lysis buffer. Dissocaited single cells were washed, filtered and resuspended in 0.4% BSA in PBS and adjusted to concentration of 1000 cells/uL for single cell RNAseq library construction. All samples used the 10x Chromium transcriptomics platform (10x Genomics) implemented by the Brigham and Women's Hospital Single Cell Genomics Core. The 12dpa library was made with the 10x Chromium Single Cell 3' v2 chemistry, and all other libraries (11dpa, 14dpa, 17dpa, and unamputated) were made with the 10x Chromium Single Cell 3' v3 chemistry as per manufacturer's protocol.
GEO Accession
GSM4276219