GSM1261020: TNBC5; Homo sapiens; RNA-Seq
Whole transcriptome RNA was extracted from total RNA by removing large and small ribosomal RNA (rRNA) using RiboMinus Eukaryote Kit (Invitrogen, Carlsbad, CA). Five micrograms of total RNA was hybridised to rRNA-specific biotin labelled probes at 70°C for 5 minutes. The rRNA-probe complexes were then removed by streptavidin-coated magnetic beads. The rRNA-free transcriptome RNA was concentrated by ethanol precipitation. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM1261020