Transcriptome of desiccating L. ohadii filaments by RNAseq, winter conditions, time 60 minutes, replicate 1
Planktonic net discs (70 mm diameter) loaded with Leptolyngbya filaments corresponding to 0.5 mg chlorophyll were incubated in 15°C on 1.5% agar plate with BG11 medium [33] with light (20 µmol photons m-2 s-1) for 12 h and dark for the other 12 h. On the day of the experiment the filter was taken from the incubator after 6 h or more of dark and placed in the environmental chamber. The dark and the relatively low temperature were important in order to prevent significant changes in the conditions at the beginning of the simulation where temperature in the field was about 15°C. The sample was then dried in the environmental chamber simulating morning conditions of October 14, 2009 (autumn) or December 8, 2016 (winter) without or with the addition of far red illumination.