GSM3143783: HeLa Rep1; Homo sapiens; OTHER
DNA samples were digested with DNase, followed by end-repair, dA-tailing, ligation of bridge adaptors, phosphorylation, and in situ ligation. After reversing crosslinks, genomic DNA was purified, sheared using a Covaris S-220 sonicator, and subjected to streptavidin-based affinity purification to recover biotinylated fragments. NGS libraries were generated by dA tailing, ligation of sequencing adaptors, PCR amplification, and Ampure beads-based purification. Finally, libraries were sequenced on an Illumina HiSeq 4000.
GEO Accession
GSM3143783