GSM4057245: Phf7 f/f rep1; Mus musculus; RNA-Seq
Mouse testis tissues were digested with collagenase A, DNase I , and trypsin in DMEM-F12. Trypsin was blocked using FBS and cells were filtered by 70 μm and 40 μm strainers. Following centrifugation, red blood cells were removed using RBC lysis buffer. Then, testis cells were washed and resuspended with HBSS. The library was constructed using the Chromium Single Cell 3' Library & Gel Bead Kit v3. The experimental process was conducted according to manufacturer's instruction without modification. The final libraries were sequenced on Nextseq 550.
GEO Accession
GSM4057245