CLIP-seq analysis of FUS in Neuro2A cells
CLIP-seq was performed using anti-FUS mouse monoclonal antibody (4H11, Santacruz), following the method developed by Darnell and coworkers (Chi, S. W. et al. 2009) with some modifications, as preveously described (Masuda A. et al. 2012). High-throughput sequencing was performed with the SOLiD 4 sequencer (Life Technologies).