GSM1929730: MLXDN-INPUT3; Mus musculus; ChIP-Seq
For ChIP-sequencing, libraries were prepared from 10 ng DNA using the NEB Next ChIP-Seq Library Prep Reagent Set for Illumina with NEB Next High-Fidelity 2x PCR Master Mix according to the manufacturer’s instructions (New England Biolabs) with the following modifications: a second 1:1 Ampure cleanup was added after adaptor ligation; the Ampure size selection step prior to PCR was eliminated; the 72C extension step of the PCR was lengthened to 45 s. Completed libraries were analyzed for insert size distribution on a 2100 BioAnalyzer High Sensitivity kit (Agilent Technologies) or Caliper LabChip GX DNA High Sensitivity Reagent Kit (PerkinElmer) Libraries were quantified using the Quant-iT PicoGreen dsDNA assay (Life Technologies) and Kapa Library Quantificaiton kit (Kapa Biosystems) or low pass sequencing on a MiSeq nano kit (Illumina). Fifty cycle single end sequencing was performed on an Illumlina HiSeq 2500 for DNA obtained from MLX-WT and MLX-DN samples immunoprecipitated for acetylated histone H4 with three replicates for each sample including input chromatin as control.
GEO Accession
GSM1929730