GSM3602650: Sp1_deltaDBD; Mus musculus; RNA-Seq
Single cell suspensions of Sp1 wild-type and DNA binding domain mutant cell were sorted for Kit (KIT-APC; BD Pharmingen 553356), expression at day 2 of blast culture prior to loading 4000 single cells of each population on a Chromium Single Cell Instrument (10X Genomics). Library generation for scRNA-seq was performed as a service by the Genomics Birmingham Sequencing Facility using the Chromium Single Cell 3' Library & Gel Bead Kit v2 (10X Genomics). Libraries were subjected to paired end sequencing on an Illumina NextSeq 500 sequencer according to 10X Genomics recommended cycle parameters.
GEO Accession
GSM3602650