Illumina HiSeq 2500 sequencing; RNA-seq of human cell line U2OS treated with chloroquine, amodiaquine and BMH-21
RNA-seq of human cell line U2OS treated with chloroquine, amodiaquine and BMH-21
U2OS cells were lysed after 12 hours of incubation to further proceed with RNA extraction Cells cultured in DMEM 10% fetal bovine serum, penicilin/streptomycin, 5% CO2 Cells were treated with the indicated compounds and concentrations in four 6-well plates at confluency lower than 70% before RNA extraction Total RNA was purified using the PureLink® kit following the manufacturer's instructions (Thermo Fisher Scientific)  Illumina TruSeq Stranded mRNA with Poly-A selection
Experimental Factor: compound
DMSO
Experimental Factor: dose
0.05%