RNA-Seq of Tridrepana flava
NucleoSpin RNA II Total RNA Isolation kit (catalog no. 740955; Clontec) were used to generate RNA extracts. RNA sample quality was assessed with an Agilent 2100 Bioanalyzer and Qubit 2.0 Fluorometer. Multiplexed libraries with twelve different barcode adaptor combinations were constructed with the TruSeq RNA Sample Prep Kit v2 (catalog no. RS-122-2001, Illumina) in the Kawahara Lab at the FLMNH.