GSM1688691: DN_V5anchor_rep2; Mus musculus; OTHER
4C chromatin was prepared by primary digestion with HindIII (NEB) enzyme and secondary digestion with NlaIII enzyme (NEB), intramolecular ligation reactions were performed with T4 DNA Ligase (NEB). Inverse PCR was performed to generate 4C library. 100ng of 4C DNA was used for sequencing. Libraries were prepared according to the Illumina protocol. After end-repair and indexed adapter ligation, fragments were size-selected using Agencourt AMPure XP beads (Beckman Coulter, Brea, CA, USA) prior to amplification. The size-selected and amplified fragment libraries were verified on a 2100 Bioanalyzer (Agilent) prior to being pooled (7-10 per lane) and sequenced
GEO Accession
GSM1688691