Digestive_tract
Total RNA was extracted from the digestive tract tissue using TRIzol reagent following the manufacturerŐs instructions. Briefly, total RNA quality was assessed using an Agilent 2100 bioanalyzer and Agilent RNA nano kit. 1_g of Total RNA was used with Ribo-Zero Kit for an rRNA depletion step followed by DNase treatment using Baseline Zero protocol. RNA-Seq libraries were then prepared with the ScriptSeq v2 RNA-seq Library Preparation Kit. DNA samples were sequenced using HiSeq 2000 at Acobiom (Montpellier, France)