Illumina MiSeq paired end sequencing
DNA amplification from roots and stolons harvested from clonal plants Glechoma hederacea with or without microorganisms inoculation.
Three independent PCR amplifications targeting different genes were conducted. Amplicons producted were then pooled for each sample (i.e, 1 sample with three different amplicons). Library was then prepared using NEB kit NEBNext ultraII DNA (96 reactions) and NEB dual index multiplex oligos. There is 3 different insert sizes (424, 530, 940) in each sample since three pairs of primers were used and amplicons were then pooled. Three  forward md5: Bacteria (5'==>3') 16S V4-V7 799f: AACMGGATTAGATACCCKG, Fungi (5'==> 3')  SSU_817 F: TTAGCATGGAATAATRRAATAGGA, Archaea (5'==> 3') AR_20F ACACTGACGACATGGTTCTACATTCCGGTTGATCCYGCCRG. Three reverse md5: Bacteria (5'==>3') 16S V4-V7 1223r: CCATTGTAGTACGTGTGTA, Fungi (5'==> 3')   ns22bR: AATTAAGCAGACAAATCACT, Archaea (5'==> 3') AR_958R TACGGTAGCAGAGACTTGGTCTYCCGGCGTTGAMTCCAATT