GSM4669532: PTH2; Mus musculus; RNA-Seq
Prior to RNA isolation, cells were washed 3 times with cold PBS. RNA was isolated using 1 ml of TRIzol reagent following the manufacturer's instructions(Thermo Fisher Scientific, Mississauga, ON). RNA-Seq libraries were built with TruSeq Stranded mRNA Library Prep Kit from Illumina following the manufacturer's instructions. Pair-end reads of 100 base pairs were obtained on a HiSeq 2000 sequencing system (Illumina). The Cufflinks program was used to assemble aligned RNA-Seq reads into transcripts and to estimate their abundance (FPKM).
GEO Accession
GSM4669532