GSM2652494: 12 hour untreated cell 6 [Sample_2_6]; Homo sapiens; RNA-Seq
Single cells were hand picked with a micromanipulator and ejected into SMARTseq2 cell lysis buffer. Reverse transcription and PCR amplification occured according to the SMARTseq2 protocol as described by Picelli et al. 2014. The Illumina Nextera XT DNA Library Prep Kit (Cat#FC-131-1096) and the Index Kit v2 set A (Cat#FC-131-2001) were used with 0.3 ng of amplified DNA per cell for the construction of sequencing libraries. RNA libraries were manually prepared for sequencing. We created equimolar solutions from 96 uniquely indexed cells.
GEO Accession
GSM2652494