Illumina HiSeq 2000 sequencing; Direct RNA Sequencing of polyadenylated RNA & high-depth Illumina strand-specific, short read RNA-seq of a stably RNAi-silenced nuclear 5'-3' exonuclease XRN3 mutant line in Arabidopsis thaliana
Direct RNA Sequencing of polyadenylated RNA & high-depth Illumina strand-specific, short read RNA-seq of a stably RNAi-silenced nuclear 5'-3' exonuclease XRN3 mutant line in Arabidopsis thaliana
Seedlings were collected after 14 days of growth. Seeds were surface-sterilized, stratified and grown on Murashige and Skoog (MS) medium supplemented with 1% (w/v) sucrose and 0.3% phytagel, under a 16 h light/8 h dark photoperiod. Total RNA was extracted using Trizol reagent (Sigma). Small RNA Sample Prep Kit v1.5, including PAGE selection of small RNAs (18-30 nt).
Experimental Factor: xrn3-8 mutant
genotype
Experimental Factor: small RNAs
fraction
Experimental Factor: Illumina 50bp single-end sequencing
protocol