RNA-Seq of mus musculus
RNA was treated with RQ1 DNase (Promega, Madison, WI) for an hour and then purified using miRNeasy kit (Qiagen, Germantown, MD). 5  g of each sample was then processed using Ribozero (ScriptSeq Complete kit; Epicenter, Illumina) to deplete ribosomal RNA. Libraries were generated and sequenced on an Illumina HiS-eq for 100 bp paired reads.