GSM1875348: BMDMs_UT_wdr82sh_sh1137_intergenic_dx_Mpeg1; Mus musculus; OTHER
4C templates were prepared as previously described (Splinter et al., 2012).  Briefly, chromatin was cross-linked with 2% formaldehyde for 10 min at room temperature, nuclei were isolated and cross-linked DNA was digested with the 4bp cutter DpnII. After proximal fragments ligation, cross-links were reverted. The second digestion was performed with the 4bp cutter Csp61 and followed again by intramolecularligation. 200 ng of the obtained 4C template were used to perform 8 individual PCR reactions, which were then pooled together and purified for HT sequencing. Adaptor sequences necessary for Illumina single-read sequencing (Hi-seq 2000) were added to 4C primer pairs as additional 5′ overhangs
GEO Accession
GSM1875348