Histone H3K27ac ChIP-Seq of CD3 Primary Cells
Single read - Illumina
EXPERIMENT_TYPE
Histone H3K27ac
EXTRACTION_PROTOCOL
http://www.roadmapepigenomics.org/protocols/type/experimental
EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR
Diagenode Bioruptor
EXTRACTION_PROTOCOL_SONICATION_CYCLES
20-40 cycles of 0.5 minute on and 0.5 minute off
CHIP_PROTOCOL
http://www.roadmapepigenomics.org/protocolstype/experimental
CHIP_PROTOCOL_CHROMATIN_AMOUNT
60ug
CHIP_PROTOCOL_BEAD_TYPE
Invitrogen Dynabeads
CHIP_PROTOCOL_BEAD_AMOUNT
48ul
CHIP_PROTOCOL_ANTIBODY_AMOUNT
3ug
CHIP_ANTIBODY
Histone H3K27ac
CHIP_ANTIBODY_PROVIDER
Active Motif
CHIP_ANTIBODY_CATALOG
39133
CHIP_ANTIBODY_LOT
21311004
GEO Accession
GSM1058764