GSM4669534: miR_I1L3_scRNA-seq_D0; Homo sapiens; RNA-Seq
At Day 0, 5, 10, 15 and 20 days into reprogramming, cells were washed with sterile PBS, detached using Trypsin, and collected by centrifugation in complete neuronal media supplemented with 5% FBS. Cells were counted using a hemocytometer and were resuspended in Cryostar CS10 cryopreservation medium at a concentration of 100 cells per μL. These aliquots were placed in a cryogenic freezing container and stored at -80°C until all timepoints were collected (Figure 1). For single-cell library preparation, we used the 10x Genomics platform with the Chromium Single Cell 3′ Reagents Kits according to manufacturer instructions. cDNA libraries were quantified on an Agilent BioAnalyzer. This timecourse single cell preparation was sequenced on an Illumina HiSeq 2500.
GEO Accession
GSM4669534