mRNA-seq of Mus musculus testis
Total RNA was used to generate polyA-selected libraries with Kapa mRNA HyperPrep kits (Roche) with indexed adaptors. Libraries were assessed on the Tapestation 2200 (Agilent) and quantitated by Kapa qPCR. Pooled libraries were subjected to 50 bp paired-end sequencing according to the manufacturers protocol (Illumina NovaSeq6000). Bcl2fastq2 Conversion Software (Illumina) was used to generate de-multiplexed Fastq files.