GSM3143096: Murine pancreatic cancer cell line p53R172H ON Replicate 3; Mus musculus; RNA-Seq
For all cells, RNA was extracted using Qiagen RNeasy columns. poly(A)-selected, unstranded Illumina libraries were prepared with a modified TruSeq protocol. 0.5× AMPure XP beads were added to the sample library to select for fragments <400 bp, followed by 1× beads to select for fragments >100 bp. These fragments were then amplified with PCR (15 cycles) and separated by gel electrophoresis (2% agarose). 300 bp DNA fragments were isolated and sequenced on the Illumina HiSeq 2000 (~100M 2×49 bp reads per sample).
GEO Accession
GSM3143096