WES of homo sapiens
Sequencing library was prepared with Illumina Truseq Exome Library Prep kit. Briefly, genomic DNA samples were normalized to 100 ng for sonication to produce fragment of approximately 200 bp in length. Library construction was performed individually with unique index for each sample. Next, two rounds of hybridization and wash were performed. Up to 12 libraries with unique index could be mixed with equal amount and capture the exon fragment. Following several cycles of PCR, the library mixture was ready for quality check and quantification. After concentration adjustment, library mixture was denatured and diluted for next-generation sequencing, which was performed using a NextSeq 500 sequencer.