GSM1692839: RNA-seq; Mus musculus; RNA-Seq
Total RNA was extracted using Trizol (Invitrogen) according to manufacturer's instruction. Ribosomal RNA was removed using RiboMinus Eukatyote Kit (Invitrogen). The mRNA libraries were prepared for strand-specific sequencing as described previously (Parkhomchuk et al. 2009). DNA was end-repaired using End-It DNA End-Repair Kit (Epicenter) and 3' ends were adenylated by treating the samples with Klenow (exo-) (NEB) in the presence of dATP.  The samples were then ligated to Illumina TruSeq adapters. The ligated products were run on agarose gel and were purified using Qiagen MinElute columns. The ligated products were then PCR-amplified using KAPA master mix. The amplified products were run on agarose gel and were purified using Qiagen MinElute columns.
GEO Accession
GSM1692839