GSM4217688: mESC, UV4I; Mus musculus; RIP-Seq
Crosslinked cells were collected by scrapping, lysed by sonication, and Sox2-RNA complexes were immunoprecipated with magnetic beads. Sox2-RNA complexes were disrupted by reverase crosslinking and proteinase K treatment, RNA was isolated using Trizol, treated with DNAse, and purified with Qiagen Rneasy MinElute Cleanup kit. The commerical kit (Kapa RNA HyperPrep with RiboErase, KK8560) was used with IDT xGen dual index UMI adaptors. Ribosomal RNA was depleted followed by cDNA synthesis.
GEO Accession
GSM4217688