GSM1608568: Sus1-WT heat shock rep2; Saccharomyces cerevisiae; ChIP-Seq
Cross-linked chromatins were sheared using a sonicator, and immunoprecipitated using the following beads or anti-body: Sepharose to pull down all myc-tagged proteins used in this study. Mock ChIP DNA was prepared by immunoprecipitation with myc-antibody in the wild type strain with no myc-tagged protein expression. ChIP-exo experiments were carried out essentially as described (PMID: 22258509). This included an immunoprecipitation step with magnetic beads, followed by DNA polishing, A-tailing, Illumina adaptor ligation (ExA2), and lambda and recJ exonuclease digestion on the beads. After elution, a primer was annealed to EXA2 and extended with phi29 DNA polymerase, then A-tailed. A second Illumina adaptor was then ligated, and the products PCR amplified and gel-purified.
GEO Accession
GSM1608568