GSM2474390: nod_top3_0425; Mus musculus; RNA-Seq
Subpopulations of beta cells (Top and Btm) were sorted from 8-10-wk-old NOD mice. Cell viability and concentration was assessed by the Countess Automated Cell Counter (ThermoFisher Scientific). Reverse transcription and cDNA amplification were performed from approximately 1,000 cells using SMARTer Ultra Low RNA kit (Clontech). Sequencing libraries were prepared using Nextera XT DNA Sample Preparation kit (Illumina). Libraries were pooled and sequenced.
GEO Accession
GSM2474390