Incomplete recovery and individualized responses of the human distal gut microbiota to repeated antibiotic perturbation - Subject DE
Proximal spacer:GG;   Proximal primers (533-515 reverse): TACCGCGGCTGCTGGCAC, TACCGCGGCGGCTGGCAC; Proximal spacer + primer variants accepted for quality filter (reads lacking an expected variant at the proximal end were rejected):  GGTACCGCGGCTGCTGGCAC, GGTACCGCGGCGGCTGGCAC, GTACCGCGGCTGCTGGCAC, GTACCGCGGCGGCTGGCAC, GGGTACCGCGGCTGCTGGCAC, GGGTACCGCGGCGGCTGGCAC; Distal spacer: CA;  Distal primers(8-27 forward): AGAGTTTGATCATGGCTCAG, AGAGTTTGATCCTGGCTCAG, AGAGTTTGATTCTGGCTCAG, AGGGTTCGATTCTGGCTCAG; Distal spacer + primer recognition not required for binning/trimming/filtering.       Sample Barcode (a' and 'b' suffix indicate replicate PCR reactions under identical conditions;   5n' suffix indicates replicate PCR with 5 ng template DNA instead of standard amount of 50 ng;   6m' suffix indicates replicate PCRs separated by 6 month interval) D6/TAGTGTAGAT;  D7/TCGATCACGT;  D9_a/TCGCACTAGT (replicate PCR identical conditions); D9_b/CGTGTCTCTA (replicate PCR identical conditions); D9_5n/ACACATACGC (replicate PCR 5 ng template DNA instead of 50 ng); D10_a/TCTAGCGACT (replicate PCR identical conditions); D10_b/CTCGCGTGTC (replicate PCR identical conditions); D10_5n/ACAGTCGTGC (replicate PCR 5 ng template DNA instead of 50 ng); D13_a/TCTATACTAT (replicate PCR identical conditions); D13_b/TAGTATCAGC (replicate PCR identical conditions); D13_5n/ACATGACGAC (replicate PCR 5 ng template DNA instead of 50 ng); D15_a/TGACGTATGT (replicate PCR identical conditions); D15_b/TCTCTATGCG (replicate PCR identical conditions); D15_5n/ACGACAGCTC (replicate PCR 5 ng template DNA instead of 50 ng); D18_6m/CGAGAGATAC (replicate PCR separated by 6 month interval); D19_a/TGTGAGTAGT (replicate PCR identical conditions); D19_b/TGATACGTCT (replicate PCR identical conditions); D19_5n/ACGTCTCATC (replicate PCR 5 ng template DNA instead of 50 ng); D21_a/ACAGTATATA (replicate PCR identical conditions); D21_b/TACTGAGCTA (replicate PCR identical conditions); D21_5n/ACTCATCTAC (replicate PCR 5 ng template DNA instead of 50 ng); D22/ACGCGATCGA;  D22_5n/ACTCGCGCAC (replicate PCR 5 ng template DNA instead of 50 ng); D33/ACTAGCAGTA;  D34/AGCTCACGTA;  D36/AGTATACATA;  D36_5n/ACGAGTGCGT (replicate PCR 5 ng template DNA instead of 50 ng); D37/AGTCGAGAGA;  D37_5n/ACGCTCGACA (replicate PCR 5 ng template DNA instead of 50 ng); D40/AGTGCTACGA;  D40_5n/AGACGCACTC (replicate PCR 5 ng template DNA instead of 50 ng); D42/CGATCGTATA;  D42_5n/AGCACTGTAG (replicate PCR 5 ng template DNA instead of 50 ng); D46/CGCAGTACGA;  D46_5n/ATCAGACACG (replicate PCR 5 ng template DNA instead of 50 ng); D48/CGCGTATACA;  D48_5n/ATATCGCGAG (replicate PCR 5 ng template DNA instead of 50 ng); D49/CGTACAGTCA;  D51_6m/CAGTAGACGT (replicate PCR separated by 6 month interval); D52_6m/CGACGTGACT (replicate PCR separated by 6 month interval); D53_6m/TACACACACT (replicate PCR separated by 6 month interval); D55_6m/TACAGATCGT (replicate PCR separated by 6 month interval); D56_6m/TACGCTGTCT (replicate PCR separated by 6 month interval); E6/CGTACTCAGA; E7/CTACGCTCTA;  E9/CTATAGCGTA;  E10/TACGTCATCA;  E13/TAGTCGCATA;  E19/TATATATACA;  E20/TATGCTAGTA;  E32/TCACGCGAGA;  E33/TCGATAGTGA;  E35/TCGCTGCGTA;  E38/TCTGACGTCA;  E40/TGAGTCAGTA;  E44/TGTAGTGTGA;  E46/TGTCACACGA;  E52/TGTCGTCGCA  