GSM2562090: SuVar_Dam_BR_F_Rep1; Drosophila melanogaster; OTHER
For DamID, salivary glands and brain ganglia were isolated from sexed third-instar larvae. Stage 10 egg chambers were isolated from adult females aged for 3 days. Testes were obtained from 3 day old males. Flies were kept at all times at 18С on standard flyfood. Organs were excised and collected in phosphate buffer saline. For each sample 100 salivary glands and brain ganglia, 50 testes and 250 egg chambers were collected. One microgram of genomic DNA was processed for library preparation using the DNA TruSeq kit. The libraries were sequenced on the Illumina MiSeq (or HiSeq) system.
GEO Accession
GSM2562090