RNA seq of Eulimnogammarus verrucosus, LC10 cadmium, 24 hours, sample 4
Total RNA for RNA sequencing was extracted from either a single individual or from a pool of up to 5 individuals (see individual BioSample description). Amphipods were homogenized in 1 ml of Qiazol Reagent (Qiagen) with MM400 homogenizer (Retsch). RNA isolation from the aqueous phases was performed with a Qiacube (Qiagen) and miRNeasy kit (Qiagen), then mRNA was isolated from the total RNA sample using Oligotex mRNA Mini Kit (Qiagen). The quality of RNA was checked with RNA 6000 Nano Kit on the Agilent Bioanalyzer 2100. mRNA libraries were prepared with ScriptSeq v2 RNA-Seq Library Preparation Kit (Epicentre, # SSV21124).