Human nasal ITS2 sequencing: Sample M1004421
Total genomic DNA was extracted using the PowerMag Microbiome DNA isolation kit. The nuclear ribosomal Internal Transcribed Spacer 2 (ITS2) region was amplified by PCR using modified ITS3 and ITS4 primers containing adapters for MiSeq sequencing and a 12-mer molecular barcode.  Individual libraries constructed from each sample were pooled and sequenced on the Illumina MiSeq platform using the 2x300 bp paired-end read protocol.