Illumina HiSeq 2500 sequencing; Using mitoribosomal profiling to investigate human mitochondrial translation
Using mitoribosomal profiling to investigate human mitochondrial translation
Cell lysates were loaded on sucrose gradient (7-47%). Fraction containing mitochondrial monosome were collected to isolated RNA. Cells were grew in DMEM medium with supplemented with 10% FCS in 5% CO2 at 37°C Cells were treated with chloramphenicol for 15 mins before harvest. Trizol extraction Digested fragments were gel-purified and dephosphorylated. 3'and 5' linker were added with T4 RNA ligase.
Experimental Factor: disease
osteosarcoma
Experimental Factor: cell line
143B