GSM4668331: IPS-8505C [8i-RNA_S4]; Homo sapiens; RNA-Seq
Total RNA was extracted from cells with RNeasy Mini Kit (Qiagen, Cat# 74104). RNA-free DNA was isolated from cells with DNeasy Blood & Tissue Kit (Qiagen, Cat# 69504). The quality of DNA was tested by Qubit Fluorometer. Next Generation Sequencing was performed using an Illumina NextSeq 500 with TruSeq Methyl Capture EPIC Library Prep Kit and KAPA HiFi HotStart Uracil ReadyMix PCR Kit. Then the sequencing data was analyzed via Integrative Genomics Viewer (http://software.broadinstitute.org/software/igv/).
GEO Accession
GSM4668331