GSM3839807: Oxy_MNase-seq_invitroSalt_methylDNA_XenOctamers_HighOct_HeavyDigest_replAB; Oxytricha trifallax; MNase-Seq
Oxytricha in vitro MNase-seq protocol: Chromatin was reconstituted in vitro through salt gradient dialysis or Nap1 histone chaperone (+/- ACF +/- ATP). Assembled chromatin was adjusted to 50 mM Tris pH 7.9, 5 mM CaCl2 and digested with a titration of MNase (New England BioLabs) to mainly mononucleosomal DNA. Mononucleosome-sized DNA was gel-purified and used for paired-end sequencing on an Illumina HiSeq 2500, according to manufacturer's instructions. MNase-seq (Paired-end sequencing); standard TruSeq Illumina library construction protocol
GEO Accession
GSM3839807