mRNAseq of specific Brassica oleracea accession: Sample GT110266
"The libraries were normalised and equimolar pooled to 10 nM using elution buffer (Qiagen) . The library pool was then diluted to 2 nM with NaOH and 5?L transferred into 995?L HT1 (Illumina) to give a final concentration of 10pM.  Each pool was spiked with 1% PhiX Control v3 and the flow cell was clustered using TruSeq SE Cluster Kit v3-cBot-HS. After the clustering procedure, the flow cell was loaded onto the Illumina HiSeq 2500 instrument following manufacturer's instructions. The sequencing chemistry used was TruSeq SBS Kit v3-HS using HiSeq Control Software 2.2.58 and RTA 1.18.64. Reads in bcl format were demultiplexed based on the 6 bp Illumina index by CASSAVA allowing for a one base-pair mismatch per library, and converted to FASTQ format by bcl2fastq."