Illumina HiSeq 2000 sequencing; RNA-sequencing for functional prediction of RNAs
RNA-sequencing for functional prediction of RNAs
Primary fibroblasts were derived from adult CAST/EiJ x C57/Bl6 mice. Tail explants were cultured in fibroblast medium (DMEM high glucose, 10% ES cell FBS, 1% Penicillin/Streptomycin, 1% non-essential amino acids, 1% sodium-puryvate, 0.1mM b-Mercaptoethanol). Cells were passaged twice before distributed as individual cells into wells of 384 or 96 well plates using a FACS. Wells was pre-loaded with lysis buffer. Cell lysis Smart-seq2, indexed by Nextera i5 and i7, and without exogenous spike-ins.
Experimental Factor: single cell identifier
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