GSM3478108: RFPHigh-Repl.1; Mus musculus; RNA-Seq
Cells were treated with TRIzol and followed with phase seperation,  RNA precipitation and RNA wash. Finally, RNA were dissolved in DEPC water. Library was constructed with NEBNext® Ultra™ Directional RNA Library Prep Kit. Briefly, total RNA was subjected to two rounds of purification using poly-T oligo-attached magnetic beads before the synthesis of double-stranded (ds) cDNA. The ds cDNA was ligated to adaptors and sequenced with illumina HiseqXten platform.
GEO Accession
GSM3478108