small-RNA-Seq
Small RNA was extracted using the mirVana miRNA Isolation Kit, ligated to custom Illumina adapters using a method that selects for small RNAs with 3' hydroxyls and 5' monophosphates, reverse transcribed, PCR amplified, and sequenced. The 5' adapter include a barcode, "CGT", which appears as the first three nt in each read from this library. Other libraries with different barcodes were pooled into the same lane in a single sequencing run.