GSM1876187: Confluent_MCF7; Homo sapiens; ChIP-Seq
Harvested MCF-7 cells were fixed with 1% formaldehyde for 15 min and quenched with 0.125 M glycine.  Lysates were clarified from sonicated nuclei and FOXM1-DNA complexes were isolated with antibody (Santa Cruz, sc-501, Lot. D0110). ChIP and Input DNAs were prepared for amplification by converting overhangs into phosphorylated blunt ends and adding an adenine to the 3’-ends. Illumina genomic adapters were ligated and the sample was size-fractionated (200-250 bp) on a 2% agarose gel. After a final PCR amplification step (18 cycles), the resulting DNA libraries were quantified and sequenced on Illumina GAII (36 nt reads, single-end).
GEO Accession
GSM1876187