GSM2993320: 1001700609_E12; Homo sapiens; RNA-Seq
No extraction, single cells are sorted into 0.4ul lysis buffer Cells are lysed in lysis buffer containing Triton X-100, then RT using template switching oligo and preamplification for 21 cycles as in SmartSeq2 is performed. Cells with different levels of intracellular virus are cherry picked and sequencing libraries are made using illumina's Nextera XT kit.
GEO Accession
GSM2993320