Histone H3K4me1 ChIP-Seq of CD3 Primary Cells
Single read - Illumina
EXPERIMENT_TYPE
Histone H3K4me1
EXTRACTION_PROTOCOL
http://www.roadmapepigenomics.org/protocols/type/experimental
EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR
Diagenode Bioruptor
EXTRACTION_PROTOCOL_SONICATION_CYCLES
20-40 cycles of 0.5 minute on and 0.5 minute off
CHIP_PROTOCOL
http://www.roadmapepigenomics.org/protocolstype/experimental
CHIP_PROTOCOL_CHROMATIN_AMOUNT
60ug
CHIP_PROTOCOL_BEAD_TYPE
Millipore A/G agarose beads
CHIP_PROTOCOL_BEAD_AMOUNT
30ul A/G bead slurry
CHIP_PROTOCOL_ANTIBODY_AMOUNT
3ul
CHIP_ANTIBODY
Histone H3K4me1
CHIP_ANTIBODY_PROVIDER
Active Motif
CHIP_ANTIBODY_CATALOG
39297
CHIP_ANTIBODY_LOT
21008001
GEO Accession
GSM1058778