GSM4552082: CS2354_nht1_rep2_RNA; Schizosaccharomyces pombe; RNA-Seq
Total cellular RNA was isolated from log-phase cells using MasterPure yeast RNA purification kit (Epicentre) according to the manufacturer's protocol. the total RNA was treated with RiboMinus™ Transcriptome Isolation Kit (ThermoFisher Scientific, K155003) to deplete rRNA. The rRNA-depleted samples were used to construct strand-specific sequencing libraries with the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB, E7760).
GEO Accession
GSM4552082