GSM1611130: Neonatal naïve; Mus musculus; RNA-Seq
Cells were sorted into Trizol, stored at -80, then RNA was extracted according to the standard Trizol protocol. PolyA RNA was selected with a biotinylated polyT primer that contained a single RNA base  and magnetic beads. After first strand synthesis, the cDNA was nicked then nick translated. After cleaning up the ends, sequencing adapters were ligated on, the libraries were amplified, and purified from PAGE gels.
GEO Accession
GSM1611130