GSM3477237: sALS patient JR61 replicate 1; Homo sapiens; RNA-Seq
RNA was extracted from each of the flash-frozen patient samples using the Ambion PureLink RNA Mini kit. RNA was assessed using the Bioanalyzer to ensure RNA Integrity (RIN) values of > 5.5. RNA-seq libraries were prepared from 500 ng of total RNA using the Illumina Tru-seq Stranded RNA kit.  Samples were barcoded to multiplex 8 samples per batch, randomly mixing ALS and Control samples in each library preparation and sequencing batch.
GEO Accession
GSM3477237