GSM3143177: IN-wholefishtranscriptome_RNAseq rep4; Danio rerio; RIP-Seq
RNA was isolated from the immunoprecipitated and input samples using the Qiagen RNeasy Plus Micro kit (Cat#74034).HC translatome was enriched for by ribosome immunoprecipitation using an HA antibody (see manuscript for full protocol) Libraries were prepared from 25ng of RNA using the TruSeq RNA Sample Prep kit (Illumina) per manufacturer's instructions, with an additional PCR cycle.RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM3143177