GSM2562035: Nrd_Rap_rep2; Saccharomyces cerevisiae; RIP-Seq
Cells were irradiated with UV light to crosslink the HTB tagged protein of interest to RNA. Cells were frozen and cracked to isolate the RNA bound proteins. Extracted RNA bound proteins were isolated 5' and 3' adapters were ligated before reverse transcribing to cDNA. Resulting cDNA was PCR amplified and multiplexed before submitted for illumina Hi-Seq sequencing and analysis. Resulting Data was demultiplexed for us.
GEO Accession
GSM2562035