Illumina HiSeq 2500 sequencing; miRNA profiling by high throughput sequencing of 60 malignant pleural mesothelioma
miRNA profiling by high throughput sequencing of 60 malignant pleural mesothelioma
samples were collected from frozen malignant pleural mesothelioma (MPM)  miRNA-Seq libraries are performed from at least 1ug of extracted total RNA with a RIN greater than 7. Before starting, total RNAs are purified with  miRNeasy kit wich allows the selection of the small RNA fraction less than 100b. libraries are performed according to previously established protocols [Vigneault, F. et al. High-throughput multiplex sequencing of miRNA. Curr Protoc Hum Genet Chapter 11, Unit 11.12.1–10 (2012).]
Experimental Factor: histology
biphasic mesothelioma
Experimental Factor: tumor stage
not available
Experimental Factor: clinical history
exposed to asbestos