GSM3143202: V4; Schistosoma mansoni; RNA-Seq
Parasites were left in treatment for 18 hours then removed and rinsed with RPMI, then placed in RLT plus beta mercaptoethanol at -80. RNA was harvested using RNeasy Mini Kit (Qiagen #74104).  RNA was tested on nanodrop and Bioanalzer. Illumina platform KAPA Stranded mRNA-Seq Kit (KAPABIOSYSTEMS #KK8420) was used with 500 ng of total RNA for the construction of sequencing libraries. Libraryconsruction followed the workflow of Illumina platform KAPA Stranded mRNA-Seq Protocol v.3.15.
GEO Accession
GSM3143202