MGH AF Study Resequencing Five GWAS Loci for Atrial Fibrillation: Sample RS22800
Library construction and custom capture have been automated (Perkin-Elmer Janus II) in 96-well plate format. 1 ug of genomic DNA is subjected to a series of shotgun library construction steps, including fragmentation through acoustic sonication (CovarisE220), end-polishing (New England Biolabs E6050L) and A-tailing (New England Biolabs E6053L), ligation of sequencing adaptors with 8 bp barcodes for multiplexing (Enzymatics L6030-HC-L) and PCR amplification (Bio-Rad 1725310). Libraries undergo custom capture using a Roche/Nimblegen Seqcap EZ custom designed probe. Prior to sequencing, the library concentration is determined by triplicate qPCR and molecular weight distributions verified on the Agilent Bioanalyzer (consistently 125 ± 15 bp).
alignment_software
bwa 0.5.9