GSM4218366: met/dnmt3b wgbs; Physcomitrella patens; Bisulfite-Seq
Genomic DNA was extracted from 7 days old protonema using DNeasy Plant Mini Kit (Qaigen) according to manufacturer's instructions Genomic DNA was isolated from protonema, fragmented by sonication, end repaired, and ligated to custom synthesized methylated adaptors. Adaptor-ligated libraries were subjected to bisulfite conversion treatment using the Methylation-Gold kit (ZYMO) as outlined in the manufacturer's instructions. The bisulfite-converted libraries were then amplified by PCR and gel purified (~350-400 bp size range) prior to quantification with a Bioanalyzer (Agilent).
GEO Accession
GSM4218366