Shotgun sequencing of polyA+ RNA isolated from the iPS cell line clone 19.11 derived from reprogramming of foreskin fibroblasts
SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
EXPERIMENT_TYPE
mRNA-Seq
EXTRACTION_PROTOCOL
RNeasy Blood and Tissue Kit (Qiagen)
EXTRACTION_PROTOCOL_MRNA_ENRICHMENT
Poly(A)Purist MAG Kit (Ambion)
EXTRACTION_PROTOCOL_FRAGMENTATION
SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
MRNA_PREPARATION_INITIAL_MRNA_QNTY
20-100 ng
MRNA_PREPARATION_FRAGMENT_SIZE_RANGE
200 nt
RNA_PREPARATION_5'_RNA_ADAPTER_SEQUENCE
SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
RNA_PREPARATION_3'_RNA_ADAPTER_SEQUENCE
SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
RNA_PREPARATION_REVERSE_TRANSCRIPTION_PRIMER_SEQUENCE
SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
RNA_PREPARATION_5'_DEPHOSPHORYLATION
NA
RNA_PREPARATION_5'_PHOSPHORYLATION
NA
RNA_PREPARATION_3'_RNA ADAPTER_LIGATION_PROTOCOL
SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
RNA_PREPARATION_5'_RNA_ADAPTER_LIGATION_PROTOCOL
SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
RNA_PREPARATION_REVERSE_TRANSCRIPTION_PROTOCOL
SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
LIBRARY_GENERATION_PCR_TEMPLATE
SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
LIBRARY_GENERATION_PCR_POLYMERASE_TYPE
SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
LIBRARY_GENERATION_PCR_THERMOCYCLING_PROGRAM
SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
LIBRARY_GENERATION_PCR_NUMBER_CYCLES
SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
LIBRARY_GENERATION_PCR_F_PRIMER_SEQUENCE
SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
LIBRARY_GENERATION_PCR_R_PRIMER_SEQUENCE
SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
LIBRARY_GENERATION_PCR_PRIMER_CONC
SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
LIBRARY_GENERATION_PCR_PRODUCT_ISOLATION_PROTOCOL
SOLiD Total RNA-Seq (STaR-Seq, Life Technologies)
GEO Accession
GSM706050