Illumina HiSeq 2500 paired end sequencing; RNA-seq of bovine genital ridges from male and female fetuses at three different time points corresponding to sex determination and early differentiation of the gonads
RNA-seq of bovine genital ridges from male and female fetuses at three different time points corresponding to sex determination and early differentiation of the gonads
Genital ridges were dissected away from the mesonephros in sterile PBS and stored at -80 °C until all samples were collected. Three pairs of gonads from different animals were grouped to produce each sample. RNA was extracted using a Direct-zolTM RNA MiniPrep Kit (Zymo Research, CA, USA) following the manufacturer's protocol. DNA digestion was performed with RQ1 RNase-Free DNase (Promega, WI, USA) [60]. Total RNA was eluted in 50 µl RNase-free water. Immediately after extraction, total RNA was cooled to -80 ºC until further use.   RNA-seq libraries were prepared from three D35, three D39 and three D43 genital ridges from males and females
Experimental Factor: age
35
Experimental Factor: sex
male