Saccharomyces cerevisiae IMS0634
Genomic DNA of strain IMS0634  was isolated from exponentially growing shake-flask cultures on SM (10 g L-1 glucose/20 g L-1 xylose) with a Qiagen Blood & Cell culture DNA kit (Qiagen, Germantown, MD), according to the manufacturer s specifications. Whole-genome sequencing was performed on an Illumina HiSeq PE150 sequencer (Novogene Company Limited, Hong Kong). Sequence data were mapped to the reference CEN.PK113-7D genome (Nijkamp et al. 2012), to which the sequences of the pTPI1-gndA-tCYC1 and pTPI1-xylA-tCYC1 cassettes were manually added.Qualified DNA is cut into fragments by restriction enzyme. The construction of the DNA libraries is through the processes of end repairing, adding A to tails, purification, PCR amplification and etc. Libraries are sequenced by Illumina high-throughput sequencer with paired-end sequencing strategy.