GSM2652072: Thrombin + SB + AG Replicate 3; Homo sapiens; RNA-Seq
RNA was extracted using Qiagen RNeasy kit mRNA library preparation followed the manufacturer’s instructions (Ion Chip Sequencing Kit - Thermo Fisher Scientific). QuadTrim was used for quality trimming of RNA-sequencing reads. Raw reads with Q-score greater than 20 (>Q20) were retained for further analysis. High quality filtered reads were aligned to the human reference genome (hg19) using Bowtie v2.2.4 and TopHat v2.1.1 [40, 41]. Uniquely mapped reads were then used for counting reads using HTSeq.
GEO Accession
GSM2652072