Illumina HiSeq 2000 paired end sequencing; transcriptome of holcus lanatus
transcriptome of holcus lanatus
Arsenate tolerant and non-tolerant Holcus lanatus L. Genovars were selected randomly from a screened population, the arsenic uncontaminated Cruickshank Botanic Garden (CBG) and tillers of the plants were grown hydroponically for 2 weeks in 50ml individual centrifuge tubes with base of the tubes covered in tin-foil to block out light, in heated glasshouse at around 18C under supplemental sodium lamps. Plant tillers from arsenic tolerant (T) and non-tolerant (N) plants were grown hydroponically for 2 weeks in 50ml complete Hogland solution (plusP treatment) or 50ml Hogland solution minus Phosphate (minusP treatment). The experiment therefore investigated 4 treatments (TplusP, TminusP, NplusP, NminusP) and was repeated 2 time. Experiment1 was conducted on 4 plants (n=1), Experiment2 was conducted on 16 plants (n=4). dry roots and shoots for each replicate were ground under liquid nitrogen and total RNA was extrected using the Plant Rneasy kit (Qiagen), with additional on-column Dnase treatment. Extracted RNA of each sample was stored at -80C until shipping on dry ice for sequencing 454-TITANIUM, normalised total RNA
Experimental Factor: growth condition
Hogland solution minus phosphate
Experimental Factor: phenotype
Arsenic-non-tolerant