GSM2653165: RNA NMP Single Cell CS21; Mus musculus; RNA-Seq
The IFC was processed according to the Fluidigm mRNA-Seq protocol. A 1:4000 dilution of ERCC spike-ins (Ambion) were added to the lysis buffer. Upon visual inspection, 55 capture sites were called to contain cells. The amplified cDNA was measured using the Qubit DNA HS assay and a selection of samples was verified on a Bioanalyzer DNA HS chip (Agilent). The final libraries for those 55 capture sites were prepared with the Nextera XT (Illumina) kit according to the protocol, pooled and sequenced on a NextSeq (Illumina) sequencer using 2x75bp read lengths.
GEO Accession
GSM2653165