Illumina HiSeq 2000 paired end sequencing; Expression in hypothalamus of wild-derived mice M.m. domesticus
Expression in hypothalamus of wild-derived mice M.m. domesticus
Mice were sacrificed at the age of 11 to 13 weeks. All dissections were done by the same person following personal instructions and standardized protocols designed by Valery Grinevich (MPI for Medical Research Heidelberg for HYP). Brains were removed and further dissection was performed on wet ice using gross anatomical borders (i.e., optic chiasm arms, optic tracts, and mammillary body) as markers. Prepared tissues were immediately frozen on dry ice and kept at -70 degree centigrade until RNA preparation. Using TRIzol Reagent with the PureLink RNA Mini Kit, invitrogen/ambion With On-Column PureLink DNase Treatment during Purification Illumina TruSeq RNA Sample Preparation Kit v2
Experimental Factor: strain
Koeln
LIBRARY_STRAND
not applicable