GSM1260649: MN_Scerevisiae; Saccharomyces cerevisiae W303; MNase-Seq
Mononucleosomal DNA was isolated as described (de Castro et al. 2012). The amount of Zymolyase  20T used to prepare spheroplasts was optimized experimentally to generate a 80:20 ratio of mononucleosomes to dinucleosomes, as described (Lantermann et al. 2010). Cells of S. cerevisiae were treated with 5 mg of Zymolyase 20T during 10 minutes at 30 ºC. Permeabilized cells were treated with 45 units/ml of micrococcal nuclease at 37 ºC during 10 minutes. Mononucleosomal DNA was recovered from 1.5% agarose gelsl. Mononucleosomal DNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM1260649