Scylla olivacea transcriptome
Messenger RNA isolation and cDNA synthesis were performed using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® according to manufacturer’s protocol. The synthesized cDNA was quantified using Qubit 2.0 DNA Broad Range Assay (Invitrogen, USA). The RNA extraction is from testis of maturing male S.olivacea (Index AGTTCC).