GSM3602922: GD_3; Mus musculus; RNA-Seq
Pooled secondary lymphoid organs (cutaneous and mesenteric lymph nodes, spleen) were digested with collagenase IV and DNase I and CD4 T cells enriched using StemCell EasySep Mouse T Cell Isolation Kit (Cat 19852). 4C T cells were sorted by FACS (>99% purity) and total RNA isolated using TRIzol (Invitrogen) and RNeasy Micro kit (Qiagen). Libraries were prepared with Clontech SMARTer kit. HiSeq Flow Cell v3, HiSeq control software version 2.2.38, RTA(Real-Time Analysis) version 1.18.61, bcl2fastq version 1.8.4
GEO Accession
GSM3602922