GSM1929743: Mesp1Cre WT rep4; Mus musculus; RNA-Seq
Total RNA was isolated from samples using RNAqueous-Micro Total RNA Isolation Kit (Ambion, AM1931) and followed with DNase I digestion. Total RNA was quantified on Nanodrop and quality was checked using Bioanalyzer. RNA-seq libraries were prepared with ovation RNA-seq system v2 kit (NuGEN). The double-stranded DNA was then amplified using single primer isothermal amplification (SPIA). Random hexamers were used to amplify the second-strand cDNA linearly.  Finally, libraries from the SPIA amplified cDNA were made using the Ultralow DR library kit (NuGEN). The RNA-seq libraries were analyzed by Bioanalyzer and quantified by qPCR (KAPA). For each group of mutant and WT control samples (either 6 or 8 indexed samples total), they were sequenced in two lanes on an Illumina HiSeq 2500 or Illumina HiSeq 4000 for a total of at least 360 million reads.
GEO Accession
GSM1929743