GSM3076089: 4: Flesh-6d; Cucumis sativus; RNA-Seq
Total RNA was extracted using RNAiso Plus Total RNA extraction reagent (Cat#9109, TAKARA, China) and a RNeasy Mini Kit (Cat#74106, Qiagen) following the manufacturer's instructions, the RIN number was subsequently determined to evaluate RNA integrity using an Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, US). Qualified total RNA was further purified using a RNeasy Micro Kit (Cat#74004, QIAGEN, GmBH, Germany) and a RNase-Free DNase Set (Cat#79254, QIAGEN, GmBH, Germany). The purified total RNA was then subjected to mRNA isolation, removal of rRNA, fragmentation, first-strand cDNA synthesis, second-strand cDNA synthesis, terminal repair, 3' end addition of an 'A', ligation and enrichment. Finally, sample libraries were constructed. The nucleic acid concentrations of the libraries were measured using a Qubit® 2.0 Fluorometer, and the Agilent 2100 system was used to determine the size of the library.
GEO Accession
GSM3076089