GSM1690182: second_pregnancy; Mus musculus; RNA-Seq
Total RNA was isolated using TRIZOL reagent (Invitrogen). RNA purity was assessed using the ND-1000 spectrophotometer (Nanodrop Technologies). For each total RNA sample, the A260/A280 ratio was greater than 1.8 and A260/A230 ratio greater than 2.0. RNA integrity was evaluated using the Agilent 2200 TapeStation. All total RNA samples had a RINe value above 7.0. Oligo-(dT) magnetic beads were used to isolate poly-(A)+ mRNA from total RNA. The resulting mRNA was fragmented to approximately 200 bp in fragmentation buffer. Subsequently, these short fragments were used as templates for first strand and second strand cDNA synthesis. The double-stranded cDNA fragments were purified, end-repaired and then ligated to sequencing adapters. After purification, suitable fragments were enriched by PCR amplification according to instructions of TruSeq® RNA LT/HT Sample Prep Kit (Illumina).
GEO Accession
GSM1690182