Illumina HiSeq 4000 paired end sequencing; ChIP-seq of HNF4A and GATA6 in oesophageal adenocaricnoma OE19 cells
ChIP-seq of HNF4A and GATA6 in oesophageal adenocaricnoma OE19 cells
OE19 cells were grown in RPMI media supplemented with 10% FBS and 1% PenStrep. 10 million cell per IP were collected. ChIP was carried out as described in Britton E, Rogerson C, Mehta S, Li Y, Li X, Fitzgerald RC, Ang YS, Sharrocks AD. Open chromatin profiling identifies AP1 as a transcriptional regulator in oesophageal adenocarcinoma. PLoS Genet. 2017;13(8):e1006879  ChIP-seq DNA library was prepared using Illumina TruSeq ChIP Library Prep Kit
Experimental Factor: immunoprecipitate
anti-HNF4A