rDNA-Seq: Nitrate assimilating microbes: Sample M_N14_F4_ITS
Soil DNA was extracted with FastDNA Spin kit for Soil DNA extraction kit. The V3-V4 region of the 16S rRNA gene and ITS2 of fungal ITS were amplified in triplicate using the barcoded 515fF and 806R primer set. PCR products were quantified, purified and sequenced by Ion torrent PGM platform for assessment of bacterial diversity and community structure.
alignment_software
UPARSE