GSM1611113: LSK WT replicate 3; Mus musculus; Bisulfite-Seq
Bisulphite treatment was performed using the EZ DNA Methylation Kit (Zymo Research) and upon addition of the CT conversion reagent, the incubation was conducted in a thermocycler (Eppendorf MasterCycler). Purified products were subjected to PCR amplification using the FastStart High Fidelity PCR System (Roche). PCR products were isolated using AMPure XP beads (Life Sciences). Library amplification and quality was determined AMPure XP beads (Life Sciences) and the Agilent 2100 Bioanalyzer. The amplified libaries were sequenced on an Illumina HiSeq2500 with 50 bp single-end reads following standard Illumina sequencing protocols
GEO Accession
GSM1611113