RNA-Seq of Oryctolagus cuniculus: pylorus
Total RNA from each organ was extracted using RNeasy mini kit (QIAGEN) according to the manufacturers instructions and retrotrascribed into cDNA using Maxima H Minus Double-stranded cDNA synthesis kit (Thermo Scientific). Libraries were prepared using KAPA HyperPlus kit and sequenced on an Illumina NextSeq instrument with NextSeq 500/550 Mid Output Kit v2 (2x150 bp paired-end [PE] mode; Illumina, San Diego, CA, USA).