Incomplete recovery and individualized responses of the human distal gut microbiota to repeated antibiotic perturbation - Subject E
Proximal spacer:GG;   Proximal primers (533-515 reverse): TACCGCGGCTGCTGGCAC, TACCGCGGCGGCTGGCAC; Proximal spacer + primer variants accepted for quality filter (reads lacking an expected variant at the proximal end were rejected):  GGTACCGCGGCTGCTGGCAC, GGTACCGCGGCGGCTGGCAC, GTACCGCGGCTGCTGGCAC, GTACCGCGGCGGCTGGCAC, GGGTACCGCGGCTGCTGGCAC, GGGTACCGCGGCGGCTGGCAC; Distal spacer: CA;  Distal primers(8-27 forward): AGAGTTTGATCATGGCTCAG, AGAGTTTGATCCTGGCTCAG, AGAGTTTGATTCTGGCTCAG, AGGGTTCGATTCTGGCTCAG; Distal spacer + primer recognition not required for binning/trimming/filtering.       Sample/Barcode (a' and 'b' suffix indicate replicate PCR reactions under identical conditions): E1/ACGAGTGCGT;  E2/ACGCTCGACA;  E3/AGACGCACTC;  E4/AGCACTGTAG;  E5/ATCAGACACG;  E8/ATATCGCGAG;  E11_a/CGTGTCTCTA (replicate PCR identical conditions); E11_b/TACACGTGAT (replicate PCR identical conditions); E12/CTCGCGTGTC;  E14/TAGTATCAGC;  E16_a/TCTCTATGCG (replicate PCR identical conditions); E16_b/TACAGATCGT (replicate PCR identical conditions); E17_a/TGATACGTCT (replicate PCR identical conditions); E17_b/TACGCTGTCT (replicate PCR identical conditions); E18/TACTGAGCTA;  E19/CATAGTAGTG;  E21/CGAGAGATAC;  E22/ATACGACGTA;  E23/TCACGTACTA;  E24/CGTCTAGTAC;  E25/TCTACGTAGC;  E26/TGTACTACTC;  E27/ACGACTACAG;  E28/CGTAGACTAG;  E29/TACGAGTATG;  E30/TACTCTCGTG;  E31/TAGAGACGAG;  E34/TCGTCGCTCG;  E36/ACATACGCGT;  E37/ACGCGAGTAT;  E39/ACTACTATGT;  E41/ACTGTACAGT;  E42/AGACTATACT;  E43/AGCGTCGTCT;  E45/AGTACGCTAT;  E47/ATAGAGTACT;  E48/CACGCTACGT;  E49/CAGTAGACGT;  E50/CGACGTGACT;  E51/TACACACACT;  