amplicon of granlular sludge: SBR with COD and nitrogen removal synchronously
Amplicons were extracted from 2% agarose gels and purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, U.S.) according to the manufacturerĄŻs instructions and quantified using QuantiFluor? -ST (Promega, U.S.). Purified amplicons were pooled in equimolar and paired-end sequenced (2 ĄÁ 250) on an Illumina MiSeq platform according to the standard protocols.