GSM3478731: MLS402_BRD4; Homo sapiens; ChIP-Seq
ChIP was performed by following a standard protocol. Briefly, cells were fixed with 1% formaldehyde for 10 min at room temperature, followed with 3 washes by cold PBS. Nuclei were extracted and re-suspended in SDS lysis buffer for 10 min on ice before sonication by Bioruptor (Diagenode). Optimal condition of sonication yielded genomic fragments around 200 to 500 bp. After sonication, cell debris was removed by centrifugation (13,000 rpm, 10 min). Supernatant was then pre-cleared and incubated with magnetic beads (Invitrogen Dynabeads) conjugated with specific antibodies (Supplementary Table 5).  After overnight incubation, magnetic beads were washed stepwise with cold low salt wash buffer, high salt wash buffer, LiCl wash buffer and TE buffer. Bound DNA was eluted, reverse-crosslinked, and purified by QIAquick PCR purification kit (Qiagen). Standard Illumina library preparation protocol
GEO Accession
GSM3478731