GSM4552137: cFOS_Neg_DNA_p1_C1 snRRBS; Mus musculus; Bisulfite-Seq
Following sacrafice, dentate gryus were microdissected, nuclei isolated, stained, and FACS sorted by PROX+/FOS+ and /FOS- populations into 96-well plates For single nuclei RRBS, bisulfite converted DNA was amplified using primers containing an Illumina i7 index. A different i7 index was used for every 24-nuclei pool, allowing multiplexing of 96 nuclei for paired-end sequencing on one Illumina HiSeq 2500 lane.
GEO Accession
GSM4552137