GSM2055511: Patient 81, Lesion 81, DNA methylation; Homo sapiens; MBD-Seq
Phenol chloroform DNA extraction Methylminer (Invitrogen, Catalog no. ME10025) methylated DNA enrichment. Enriched sonicated DNA was subject to size selection (350–450 bp) followed by adaptor ligation, flow cell hybridization and cluster amplification for 11–13 cycles using TruSeq SR Cluster Kit v3-cBot-HS (Illumina, Catalog no. GD-401-3001) prior to sequencing using the Illumina Hiseq2000 or Illumina Genome Analyzer IIx platform (single-end, 50 bp).
GEO Accession
GSM2055511