GSM3681651: White mussel mantle exosomes_1; Hyriopsis cumingii; miRNA-Seq
Mantles were removed, flash frozen on dry ice, and exosomes were extracted from mantle, then total RNA harvested using Trizol reagent. RNA libraries were prepared for sequencing using standard Illumina protocols The total RNA of each sample was used to prepare the miRNA sequencing library, and included the following steps: 3'-adaptor ligation, 5'-adaptor ligation, cDNA synthesis, PCR amplification, and size selection of ~150 bp PCR amplicons (corresponding to ~22 nt miRNAs). The libraries were denatured to single-stranded DNA molecules, captured on Illumina flow cells, amplified in situ as clusters, and sequenced for 50 cycles on an Illumina HiSeq sequencer
GEO Accession
GSM3681651