GSM2995122: hDicer0TRBP-pre-let-7-NAI; Homo sapiens; OTHER
35 µl of buffer RLT was added to stop the reaction on ice. The RNA was purified with an RNeasy mini column and eluted with 50 µl of pure water twice (100-µl final volume). The modified RNA was captured with DIBO-biotin at 37 °C for 2h incubation and then purified with the Zymo RNA kit. It was then subjected to end repair, linker ligation and reverse transcription. Transcribed cDNA was enriched by biotin together with the RNA moiety. cDNA libraries were amplified using qPCR with about 13-15 cycles.
GEO Accession
GSM2995122