GSM1692813: MPE-seq 10 min rep1; Mus musculus; OTHER
Nuclei were treated with MPE-Fe(II). The reaction was quenched by addition of bathophenanthroline to ~6 mM. Proteins were removed by digestion with proteinase K, extraction with phenol and chloroform, and precipitation with ethanol. RNA was removed by digestion with RNase A, extraction with phenol and chloroform, and precipitation with ethanol. DNA was end-repaired using End-It DNA End-Repair Kit (Epicenter) and 3' ends were adenylated by treating the samples with Klenow (exo-) (NEB) in the presence of dATP.  The samples were then ligated to Illumina TruSeq adapters. The ligated products were run on agarose gel and were purified using Qiagen MinElute columns. The ligated products were then PCR-amplified using KAPA master mix. The amplified products were run on agarose gel and were purified using Qiagen MinElute columns.
GEO Accession
GSM1692813