GSM4276314: CD55_nuclease_bottom; Homo sapiens; OTHER
gDNA was extracted using GeneJET DNA Purification Kit (Thermo Fisher Scientific). All of the extracted gDNA was then used in the first PCR reaction, in multiple reactions not exceeding 10 μg gDNA per 100 uL PCR  reaction. Samples were then subjected to a second PCR to add sequencing adaptors and to barcode the samples. PCR products were run on a 2% agarose gel and the correct size band was extracted. PCR products from different samples were then pooled together in equimolar ratios. Sequencing was performed on the NextSeq 500 instrument using the MidOutput Mode v2 with 75 bp paired-end reads (Illumina).
GEO Accession
GSM4276314