454 GS FLX sequencing; Equine RNAseq Tissue pool
Equine RNAseq Tissue pool
RNA was isolated using variations of guanidinium-based protocols, MacLeod JN et al. (1996) J Biol Chem 271: 18954-18960.and Chomczynski P, and Sacchi N (1987) Anal Biochem 162: 156-159 messenger RNA was isolated from total RNA with the Oligotex kit (Qiagen, Valencia, CA). Library constructed with ScriptSeq mRNA-Seq Library Preparation kit Roche Titanium-compatible (Epicentre, WI)
LIBRARY_STRAND
second strand