Roche 454 GS FLX
Total RNA extracted from 13 tissues, including heart, liver, spleen, lung, kidney, pancreas, skin, muscle, ovary, stomach, intestines, brain and arteries.We pooled equivalent amounts of total RNA from each sample (total 0.30 mg total RNA) and delivered it to Shanghai Hanyu Bio-Tech Co., Ltd. (Shanghai, China) for cDNA synthesis and libraty construction.