Gene discovery in the threatened Elkhorn coral: 454 sequencing of the Acropora palmata transcriptome
A single pooled RNA sample was sent to the Indiana University Center for Genomics and Bioinformatics (Bloomington, IN) for cDNA preparation, normalization and sequencing on the 454-GS FLX platform using Titanium chemistry following previously published methods published in: Meyer E, Aglyamova GV, Wang S, Buchanan-Carter J, Abrego D, Colbourne JK, Willis BL, Matz MV: Sequencing and de novo analysis of a coral larval transcriptome using 454 GSFlx. BMC ge-nomics 2009, 10:219.