GSM3143771: NET-seq_clr4KO_seb1-1_rep1; Schizosaccharomyces pombe; OTHER
RNA was extracted from S. pombe by ball mill (5x 3min 15Hz). RNA was isolated using Anti-FLAG M1 Agarose Affinity Gel (Sigma) and purified using the miRNeasy Mini Kit (Qiagen). Adaptor ligation was performed using random hexamer-barcoded adaptors and T4 RNA Ligase 2 Truncated K227Q. Samples were subjected to alkaline fragmentation. cDNA was prepared using Superscript III (Thermo Fisher) and circularized by CircLigase (Epicenter). The circularized cDNA was then PCR amplified to obtain our final libraries. NET-Seq
GEO Accession
GSM3143771