GSM3076160: Roots CAD mutant 1; Populus tremula x Populus alba; RNA-Seq
Total RNA was extracted from galls at 21 dai and corresponding uninfected roots from the CAD and the WT lines  (3 biological replicates) using the Plant RNA Isolation Kit (Agilent) following standard procedures with a few  modifications: an incubation step (15 min, room temperature) and a centrifugation step (5 min, 20.000 g, 4°C) were added  before prefilter column application.   RNA quality controls were performed using a BioAnalyzer RNA Nano.   All samples presented a high quality with a RIN comprised between 9 and 10 TruSeq mRNA Stranded libraries were prepared by the GIGA genomic platform (Liege University, Liege, Belgium)
GEO Accession
GSM3076160