GSM3143773: PAR-CLIP_seb1_rep1; Schizosaccharomyces pombe; OTHER
RNA-protein complexes were  isolated by ball mill (15hz, 3 minutes, 5 cycles) and purification on IgG-sepharose (GE Healthcare). Illumina RNA 3' Adapter (from the TruSeq Small RNA Sample Prep) was ligated to the Rna followed by 5' labeling with P32.  Illumina RNA 5' Adapter (from the TruSeq Small RNA Sample Prep) was then ligated. RNA-protein complexes were run on an SDS-PAGE gel, extracted, and electroeluted to isolate the complexes. RNA was isolated by proteinase K digestion, reverse transcribed and PCR amplified to produce our final libraries. PAR-CLIP and CRAC
GEO Accession
GSM3143773