GSM2652199: shCont_Atac_2; Mus musculus; ATAC-seq
50,000 mouse NeuPCs treated with plasmids harboring shRNA-GFP were sorted into ice-cold PBS, and the transposition reaction was performed. A DNA library was generated using NEBNext High-Fidelity 2x PCR Master Mix and Nextera custom primers. qPCR was used to estimate the number of PCR cycles required to determine the number of PCR cycles for 25% saturation and 10-12 cycles in total were used. The library was analyzed on Tape station analyzer (Agilent Technologies) and followed by deep sequencing with the Illumina’s NextSeq 500 at PE42.
GEO Accession
GSM2652199