GSM4217765: Snf2h_nFGO_KO_RNAseq_rep1; Mus musculus; RNA-Seq
As all libraries generated in this study involved low-input techniques, samples collected were directly put into lysis buffer for further library construction. Twenty oocytes at Growing1 (GO1), Growing2 (GO2), and fullly grown (FGO, with or without hormone stimulation) stages from WT and CKO female mice were used for RNA-seq library preparation. Briefly, reverse transcription and cDNA amplification were performed using SMARTer Ultra Low Input RNA cDNA preparation kit (Clontech) following the manufacturer's instructions. Nextera XT DNA Library Preparation Kit (Illumina) was used for cDNA fragmentation, adaptor-ligation, and amplification according to the manufacturer's instructions. Single-end 100 bp sequencing was performed on a HiSeq2500 sequencer (Illumina).
GEO Accession
GSM4217765