GSM2056843: W1-L8; Mus musculus; RNA-Seq
Snap frozen ESC and EB pellets were homogenized in Qiazol (Qiagen), and the resulting aqueous phase was further purified by the RNA clean and concentrator kit (Zymo Reasearch) and digested with DNase (Qiagen). RNA integrity (RIN) numbers were determined using the Agilent TapeStation prior to library preparation. mRNA-Seq libraries were prepared using the TruSeq RNA Library Preparation Kit (v2) according to the manufacturer’s instructions (Illumina);  non-stranded mRNA-seq
GEO Accession
GSM2056843