Campanulastrum americanum population FL83 nuclear RAD sequencing
DNA was extracted from 6 individuals from Campanulastrum americanum population FL83. After quantification individuals were evenly pooled and barcoded. The barcoded population was digested with SacI and then pooled with other populations for library construction following standard protocols for sequencing on an Illumina HiSeq. Due to low sequence complexity resulting from the restriction enzyme cut site, a 50% PhiX control was spiked into the RAD library before sequencing on two lanes of an Illumina HiSeq. Reads were run through process_radtags in Stacks to demultiplex the data and filter out low quality reads.