Genomic DNA of caenorhabditis elegans : whole worm
Genomic DNA was fragmented to ~800 bp using a Covaris M22 sonicator and checked for size by gel electrophoresis. Fragments were self-ligated using T4 ligase (NEB) and recovered using glycogen/isopropanol precipitation. Nested PCR was performed to amplify DNA sequence directly adjacent to Tc1 and Tc3 transposon ends. Two sets of primers were designed to capture sequence adjacent to both the 5 and 3 ends of the transposon. Primer sequences are provided in Supplementary Table T2. Size selection was used to obtain products under 1 kb using Mag-bind TotalPure NGS paramagnetic beads (Omega Bio-Tek). End repair and adapter ligation was carried out using reagents and protocols from the KAPA HyperPlus Library Preparation Kit (KAPA Biosystems) and Illumina TruSeq adapters. Samples were multiplexed, and sequenced on an Illumina MiSeq v3 (25M reads) paired end 300.