Small RNA-Seq from Paracoccidioides  brasiliensis Pb18: iron control - rep2
Paracoccidioides brasiliensis (Pb 18) in the yeast phase, was cultivated and maintained in Fava Netto solid medium, at 36  C. After three days, the yeast cells were inoculated in liquid Fava Netto at 36  C, under agitation (150 rpm), for 72 hours. Yeast cells were centrifuged and inoculated in modified Mc Veigh & Morton minimal medium (MMcM). MMcM medium supplemented with 3.5 M Fe (NH4)2(SO4)2, was used as control. Iron deprivation was achieved in MMcM medium supplemented with 50 M of bifophenanthroline bisulphonic acid (BPS). After 24h of growth on MMcM medium, either under control or treatment, cells were centrifuged at 12,000  g, for 10 min, the supernatant was discarded and RNAs were obtained by using TRIzol (TRI Reagent, Sigma-Aldrich, St. Louis, MO), according to the manufacturer's instructions.