Mate-pair library of Lonchura striata domestica: 9 kb insert
Mate-pair libraries were constructed using the Nextera Mate-Pair Library Preparation Kit (Illumina) with 3, 5, and 9 kb insert sizes. 4 μg purified genomic DNA was tagmented as recommended in the manufacturer's protocol then purified using the Genomic DNA Clean and Concentrator Kit (Zymo). The protocol was continued through strand displacement and size selected using BluePippin 0.75% agarose, dye-free gels (broad selection at 2000-4000 bp, 4000-6000 bp, and 8000-10,000 bp respectively). After selection, protocol was continued through final PCR amplification.