RNAseq of MEF_PD11
PD11 (population doubling times) MEF cells were obtained by isolating from mouse embryo and continously subculturing in DMEM with 10% FBS. Total RNA samples were isolated using TRIzol reagent (Life Technologies, NY, USA). The dUTP-based strand-specific RNA-seq libraries were constructed by following the protocol of Parkhomchuk et al. (NAR,2009). The concentration of the RNA-seq libraries was measured by Qubit fluorometry (Life Technologies, NY, USA) before loading onto the Illumina HiSeq2000 platform.