GSM3074888: RNA2_U251_NFD_1DPT; Homo sapiens; RNA-Seq
After cell sorting, we acquired nearly 5×10^5 U251 cells. RNA isolation was performed by TRIZOL (Life Technologies, 15596-018) followed by chloroform extraction and column-based purification kit (Zymo Research, R1016). Total RNA samples were submitted and analyzed with bioanalyzer to check RNA concentration and RNA integrity number (RIN). 1ug total RNA has been used for library preparation (Illumina, RS-122-2201)
GEO Accession
GSM3074888