GSM4217720: scRNA_SLE3_PBMC; Homo sapiens; RNA-Seq
Peripheral blood was collected by heparinized tubes and the PBMC fraction was obtained using Ficoll/Hypaque gradient.  LDGs were sorted by flow cytometry using  CD10-APC,CD14-FITC and CD15-PE antibodies. Isolated PBMCs were encapsulated using microfluidics technology, and barcoded using a unique molecular identifier (UMI) scRNAseq data were generated on Illumina HiSeq  3000 instruments according to the manufacturer's instructions
GEO Accession
GSM4217720