RNA-seq of buckwheat hypocotyls: adventitious roots formation
The explants were placed in medium with IAA (3 M) and were collected 24 h after the addition of effectors. The samples were fixed with liquid nitrogen and stored at 80C until RNA extraction. The total RNA from collected sample was extracted using a TRIzol analogue combined with an RNeasy Mini Kit (Qiagen, Germany). Polysaccharides and polyphenolics were removed from the RNA preparations by adding the Plant RNA Isolation Aid reagent (Ambion, USA) and -mercaptoethanol to the lysis buffer RLT. The preparation of cDNA library was performed using the TruSeq Sample Prep Kit v3 (Illumina, USA).