GSM3839776: 44MTB40_CD14T_S1_2; Homo sapiens; RNA-Seq
Desired monocyte populations for sorted by flow cytometry and RNA was harvested using Qiagen RNeasy micro kit and on column Dnase digestion was performed. RNA quantity and quality was analyzed on Agilent Bioanalyzer. Lexogen QuantSeq Kit was used for the construction of bulk sequencing libraries. SureCell WTA 3' Library Prep Kit was used for the construction of single-cell sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols