GSM3082050: RACHX_2h-B; Xenopus laevis; RNA-Seq
Total RNA was isolated using peqGOLD Trifast reagent (peQlab) Library preparation for RNA-Seq was performed using the TruSeq Stranded Total RNA with Ribo-Zero Gold kit removing both cytoplasmic and mitochondrial rRNA (Illumina, Cat. No. RS-122-2201, San Diego, CA, USA). About 200 ng of total RNA was used as start material. Accurate quantitation of cDNA libraries was performed  by using the QuantiFluorTM dsDNA System (Promega,Madison, WI, USA). The size range of final cDNA libraries was determined applying the DNA 1000 chip on the Bioanalyzer 2100 from Agilent (280 bp). cDNA libraries were amplified and sequenced by using the cBot and HiSeq2000 from Illumina with 50bp Single-end chemistry.
GEO Accession
GSM3082050