GSM1680904: daf-2(-);daf-12(-)-Replicate-1; Caenorhabditis elegans; RNA-Seq
Worms were grown for mixed culture from different strains on OP50 E coli. seeded 90 mm plates for RNA isolation.  The pellet was forzen in 250 µl TRIzol reagent (Invitrogen part # 15596-026) and kept at -80 °C after washing four times in M9 buffer. Total RNA was isolated as per manufacturer protocol from the frozen pellet RNA quality was checked on a 2100 Bioanalyzer by using the RNA 6000 Nano Kit (Agilent Technologies, USA). RNA with RNA integrity number (RIN) > 9 was selected for library preparation.  The RNA library was prepared using the TrueSeq RNA SamplePrep V2 kit (Illumina, USA) according to manufacturer-provided specifications.
GEO Accession
GSM1680904