Viral Metagenomes Prepared from Asteroids on North American Pacific Coast: Sample V26
"Viral metagenome prepared using the filter-chloroform-nuclease approach of Ng et al., 2011. Asteroidea tissues are homogenized in virus-free buffer, filtered to remove particles, treated with chloroform, then treated with nucleases to remove non-capsid bound DNA. After this DNA was extracted from viral purifications, amplified using GenomePlex kit (Sigma) before sequencing on Illumina 2 x 200 bp."