WGS of Astatotilapia latifasciata adult liver 2B
Genomic DNA (gDNA) from each individual was sheared to an average size of 500 bp using an S220 focused ultrasonicator (Covaris Inc., Woburn, MA). Separate libraries were constructed for each individual using the TruSeq DNA sample preparation kit ver.2 rev.C (Illumina Inc., San Diego, CA). Paired end (2x100 bp) sequencing of each library was performed in separate lanes on an Illumina HiSeq 1000 sequencer.