GSM4552166: MIAPACA2_DMSO_H3K4me2-SPIKE-IN; Homo sapiens; ChIP-Seq
For ChIP-seq experiments, cells were grown in regular medium and collected, followed by cell fixation with 1% of formaldehyde and sonication. Across all samples, the cleared cell lysates from sonicated nuclei were added with the same fraction of the sonicated and cleared chromatin of Drosophila S2 cells, and then protein-DNA complexes were isolated with specific antibodies against H3K4me2 (Millipore cat# 07-030) and those against Drosophila-specific histone H2Av (Active Motif cat# 39715). Following antibody enrichment, ChIP DNA was recovered for multiplexed library generation and parallel sequencing.
GEO Accession
GSM4552166