A-testis-neonatal3
Firstly, ribosomal RNA was removed by Epicentre Ribo-zero rRNA Removal Kit (Epicentre, USA). Subsequently, sequencing libraries were generated using the rRNA depleted RNA by NEBNext Ultra Directional RNA Library Prep Kit for Illumina(NEB, USA). The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumia). After cluster generation, the libraries were sequenced on an Illumina Hiseq 4000 platform and 150 bp paired-end reads were generated.