GSM1607164: CO2_7; Apis mellifera; RNA-Seq
Head capsules were removed and brains were dissected over dry ice. Brains were placed individually in Trizol and macerated to breakdown tissue. After addition of chloroform, samples were briefly vortexed and then centrifuged at 12000g for 15 min at 4 °C to generate an aqueous phase. After addition of isopropyl alcohol, the aqueous phase was again vortexed and centrifuged (10 min) to produce a pellet. The pellet was then washed with 75% ethanol prior to drying and redissolving in ddH2O. The dissolved RNA was treated with DNase I buffer (Ambion) to remove gDNA contaminations, prior to centrifugation and collection of the aqueous phase. The RNA content of the sample and the purity of the extracts were assessed using a NanoDrop™ 1000 spectrophotometer (Thermo Scientific Inc., Bremen, Germany). HiSeq Sequencing Kit Trueseq v3 - library construction performed at the Illumina facility following standard protocols
GEO Accession
GSM1607164