MTB IS6110 Flanking sequence for pool of clinical strains
Standard solexa library was built from 400ng of Genomic DNA, once the library pools of 12 samples were done and a PCR of the flanking sequences for the IS6110 fragments was performed. PCR products were purified and further pooled to have finally a single pool with 579 samples. Size selection was done by gel cut with an average fragment size of 250bp 