GSM1693049: CAL51MCF7_RNAseq; Homo sapiens; RNA-Seq
polyA RNA were purified using the MACS mRNA isolation kit (Miltenyi Biotec).  PolyA RNA was fragmented by RNA fragmentation reagent (Ambion), and subjected to first strand synthesis by using SupeScript II (Invitrogen) and random hexamer oligos. The second strand was synthesized, and then subjected to end repair, A-tailing, ligation of Illumia paired end adaptor. Following PCR amplification, the final library was purified with Ampure XP beads.
GEO Accession
GSM1693049