GSM1608295: EL_harr_rep2; Homo sapiens; OTHER
For ribosome profiling, cells were pretreated with cycloheximide (100 µg/ml; Sigma-Aldrich) for 90 seconds or harringtonine (2 µg/ml; Santa Cruz) for 5 minutes, and detergent lysis was then performed with flash-freezing in liquid nitrogen. DNase I-treated lysates were then treated with RNase I, and ribosome-protected fragments were purified as previously described (Ingolia et al., 2012). For ribosome profiling, ribosome-protected fragments were subjected to Illumina TruSeq library construction as previously described (Ingolia et al., 2012).
GEO Accession
GSM1608295