GSM4119617: ES_MTKO4; Mus musculus; RNA-Seq
NGS libraries were generated using V2 version of Ovation RNA-seq kit from Nugen. Libraries were prepared according to manufacturer's protocol. cDNA fragmentation was performed on Bioruptor sonication device (Diagenode) as follows: 45 s ON, 30 s OFF for 21 cycles on low intensity, 100 ng fragmented cDNA was used for further library preparation. Libraries were amplified by 9 cycles of PCR. Libraries were quantified by Qubit HS DNA kit, quality was assessed on 2100 Bioanalyzer HS DNA chip. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM4119617