RNA-seq of wild-type goosegrass
Libraries were prepared by standard methods using polyA selection from total RNA followed by random-primed first-strand and second-strand cDNA synthesis. Following cDNA synthesis, sequencing libraries were prepared using standard Illumina adaptors. The libraries were amplified using 15 cycles of PCR and following purification and size selection with Agencourt beads (Beckman Coulter Genomics), libraries were quantified via real-time PCR (Kapa Biosystems) and normalized to 12.5nM.