GSM2472404: D6-4; Prunus avium; RNA-Seq
Total RNA was extracted with the Spectrum Plant Power kit (Sigma-Aldrich) according to manufacturer’s instructions. Additions to the protocol were the following: PVPP (50 mg) was mixed with frozen ground plant material (50 mg) before the addition of lysis buffer and gDNA was degraded on column with RNAse-free DNase (Qiagen). The resulting RNA was analyzed on a Bioanalyzer (2100, Agilent). All selected samples had an RNA Integrity Numbers above 9. Total RNA was used in the Illumina TruSeq stranded mRNA library preparation kit according to manufacturer’s instructions.
GEO Accession
GSM2472404