GSM2473963: CC-FL-E105-Wt-Mm-Rep1 part1; Mus musculus; OTHER
The 3C library was prepared according to (Hagege et al. 2007), i.e. crosslinking, cell lysis, DpnII digestion, ligation and de-crosslinking. Re-ligated products were then sheared using a covaris sonicator (Duty cycle: 10%, Intensity:5, Cycles per Burst: 200, Time: 6 cycles of 60 sec each, Set Mode: Frequency sweeping, Temperature: 4° to 7°C). Adaptors were added to the sheared DNA and amplified. The adapted-ligated library was then hybridized to the custom-designed sure-select beads indexed for sequencing using an indexing PCR following Agilent instructions.
GEO Accession
GSM2473963