RNAseq of Lotus japonicus: root 5 days  IRBG74, replicate 3
mRNA was enriched  using oligo(dT) beads. mRNA was fragmented randomly by adding fragmentation  buffer, then the cDNA is synthesized by using mRNA template and random  hexamers primer, after which second-strand synthesis buffer (Illumina) ,  dNTPs, RNase H and DNA polymerase I are added to initiate the second-strand  synthesis. After terminal repair, and sequencing adaptor ligation, the  double-stranded cDNA library is completed through size selection and PCR  enrichment.