NextSeq 500 paired end sequencing; Blood Transcriptional Profiling of a prospective HIV-negative UK Cohort of Tuberculosis Contacts
Blood Transcriptional Profiling of a prospective HIV-negative UK Cohort of Tuberculosis Contacts
Close contacts of patients with active tuberculosis (TB) were recruited from multiple TB clinics in London. At enrolment, peripheral blood RNA was collected into Tempus tubes. Total blood RNA was extracted using the Tempus Spin RNA Isolation Kit (Ambion; Life Technologies). Globin mRNA and genomic DNA were removed using the GlobinClear kit (Life Technologies) and the TURBO DNAfree kit (Ambion, Life Technologies), respectively. cDNA libraries were generated using the Kapa mRNA Hyperprep kit (Roche).
Experimental Factor: progressor status (median follow-up 1.9 years)
non-progressor