GSM2813889: WT mouse ES cells 6mA DIP; Mus musculus; MeDIP-Seq
For DIP experiments, genomic DNA was isolated from wild-type ES cells using the DNeasy Blood & Tissue DNA kit and sonicated for a total of 6 min. Samples were processed using the MeDIP kit (Active Motif) according to the manufacturer's protocol. Library preparation was performed using the Kapa HyperPrep kit, followed by 12 cycles of PCR amplification with Kapa HiFi HotStart polymerase. Amplified libraries were pooled, purified twice with 0.9X Ampure XP beads and analyzed on the Illumina HiSeq2000 or NextSeq500 for single-end reads.
GEO Accession
GSM2813889