GSM2816970: MBDseq TBT rep 1; Mus musculus; MBD-Seq
Genomic DNA (gDNA) and RNA were isolated from male white gonadal adipose tissue using ZR-Duet™ DNA/RNA MiniPrep kit (Zymo Research) of animals sacrificed at 33 weeks of age (after the diet challenge). Nucleic acid samples from 4 randomly selected mice from control or TBT groups were submitted for DNA methylome and transcriptome analyses Sonicated gDNA (100-200 bp, 500 ng) was subjected to methylated DNA enrichment using the MethylMiner kit (Life Technologies), followed by deep sequencing library construction using the NEBNext Ultra kit (New England Biolabs). Sonicated gDNA without methylation enrichment was used as input control. Following the ENCODE recommendation for ChIP-seq, we aimed for 20 million mapped reads (75 nt single) for each gDNA sample
GEO Accession
GSM2816970