Illumina HiSeq 2500 paired end sequencing; RNA-seq of organ of Corti RNA from mice carrying a null allele of Mir183 and Mir96
RNA-seq of organ of Corti RNA from mice carrying a null allele of Mir183 and Mir96
4-day old pups from heterozygote x heterozygote matings were collected and culled by decapitation. The head was bisected and the brain removed. Half heads were put in RNAlater and the organs of Corti dissected out, separated from modiolus and lateral wall, and put in fresh RNAlater on ice. RNA was extracted using the QIAgen RNeasy kit with QIAshredder columns or the Lexogen SPLIT kit. Strand-specific libraries were prepared using the NuGEN Ovation Mouse RNA-Seq System 1-16 kit.
Experimental Factor: genetic modification
Mir182 knockout allele
Experimental Factor: genotype
wild type genotype