GSM1930051: WT_ribo_rep1; Mus musculus; RNA-Seq
Frozen tissues were homogenized in20 mM Tris (pH=7.5), 150 mM NaCl, 5 mM MgCl2, 1% Triton X100, 1 mM DTT, 100 µg/mL cycloheximide, 25 U/mL Turbo DNase (Ambion). RNase-treated extracts were resolved on sucrose gradients, and RNA from monosome fractions was extracted by hot phenol.  Ribosome footprints of 28-32 nt were purified by size selection and gel extraction. Footprints were dephosphorylated and ligated to a preadenylated 3' linker ((5' rAppNNNNCTGTAGGCACCATCAAT/3ddC/ 3'). Following reverse transcription, cDNA was circularized and amplified by PCR.
GEO Accession
GSM1930051