Illumina HiSeq 2500 paired end sequencing; bone marrow-derived macrophages from adult male horse
RNA-Seq transcriptomic paired end experiment using polyA selection on an Illumina HiSeq 2500
TruSeq Stranded Total RNA Sample Preparation Guide Revision E
assay type
transcription profiling by high throughput sequencing
sample storage
TRIzol, frozen
sample storage processing
cryopreservation, other
sampling to preparation interval
4
experimental protocol
https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf
extraction protocol
ftp://ftp.faang.ebi.ac.uk/ftp/protocols/assays/ROSLIN_SOP_Isolation_of_Total_RNA_from_Cells_With_DNAse_Treatment_20161116.pdf
library preparation location
Edinburgh Genomics, The University of Edinburgh, EH9 3FL, Edinburgh, Scotland
library preparation location longitude
-3.173114
library preparation location latitude
55.923953
library preparation date
2016-07
sequencing location
Edinburgh Genomics, The University of Edinburgh, EH9 3FL, Edinburgh, Scotland
sequencing location longitude
-3.173114
sequencing location latitude
55.923953
sequencing date
not applicable
experiment target
total RNA
rna preparation 3' adapter ligation protocol
https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf
rna preparation 5' adapter ligation protocol
https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf
library generation pcr product isolation protocol
https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf
preparation reverse transcription protocol
https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf
library generation protocol
https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf
read strand
mate 2 sense
rna purity - 260:280 ratio
1.91
rna purity - 260:230 ratio
2.1
rna integrity number
9.7