GSM3602339: Sample_3-fRIP-Input-Run2; Homo sapiens; RIP-Seq
100 million OCI-AML3 cells were collected by centrifugation, washed with PBS and fixed using 0.25% formaldehyde. Formaldehyde was quenched using glycine and the fixed cells were lysed using 0.5% NP40 buffer supplemented with protease and RNase inhibitors. The lysate was then flash frozen at minus 80 deg C. The lysate was then sonicated, cleared by centrifugation  and the cleared lysate was used for immunoprecipitation experiments. Libraries were prepared using TruSeq RNA Library Prepkit v2 and as per the anufacture's recommended protocol. After library prep, single-read sequencing was performed on an Illumina HiSeq 2000 at a depth of 36 nucleotides per read at the MD Anderson Sequencing Core.
GEO Accession
GSM3602339