bacterial gut microbiota from the midgut of Panesthia angustipennis, replicate 2
DNA extracted from individual gut compartments was amplified and sequenced. Briefly, the V3 V4 region was amplified with bacteria-specific primers and analyzed on a Roche GS FLX Titanium Technology instrument (GATC Biotech, Konstanz, Germany). The sequence reads (length ca. 440 bp, approximately 10 000 quality-checked reads per sample) were analyzed using mothur (Schloss et al., 2009).