RNA-Seq of Ross River virus
Design: Total viral RNA was extracted from 140l tissue culture supernatant using RNeasy-Mini kit (Qiagen), prior to being converted to cDNA using the REPLI-g WTA Single Cell kit (Qiagen). The cDNA library was prepared using the Nextera XT Kit and sequenced on a Miseq instrument using a Reagent Micro Kit v2, 300-cycles (Illumina,) performed according to the standard protocols to produce approximately a million reads (2x150 nucleotides[nt]) for each sample.