16S rRNA bacterial composition, indoor on zeigler diet d34 replicate a
Sample collected from intestine of 5 whiteleg shrimp raised indoor on zeigler diet. Shrimp d 34 of age, replicate a. Sample kept at -20C until microbial genomic DNA extractions. Microbial genomic DNA was extracted using repeated beating followed by Qiagen stool kit. PCR amplification of bacterial V1-V3 regions of 16S rRNA genes using 27F-519R primers. Sequencing using Illumina MiSeq2X300.