GSM4550352: Seq15 / KPY_EMP_3; Mus musculus; RNA-Seq
Single cell suspensions were made from organoid cultures and EPCAM+ cells were FACS sorted and immediately lysed, RNA was extracted using the protocol and reagents of the Absolutely RNA Microprep Kit (Agilent). Complementary DNA (cDNA) was synthesized with Clontech SmartSeq v4 reagents from 2ng of RNA. Full length cDNA was fragmented to a mean size of 150bp with a Covaris M220 ultrasonicator and Illumina libraries were prepared from 2ng of sheared cDNA using Takara Thruplex DNAseq reagents according to manufacturer's protocol. The finished double strand DNA libraries were quantified by Qubit fluorometer, Agilent TapeStation 2200, and RT-qPCR using the Kapa Biosystems library quantification kit. Bulk RNA-Seq
GEO Accession
GSM4550352