Hi-C of Bursaphelenchus mucronatus:egg
The extracted DNA from 20,000 isolates of mixed age were used for constructing Hi-c library. Nuclei were digested with HindIII and DpnII, marked by incubating with biotin-14-dATP, and then ligated by T4 DNA polymerase. The extracted DNA was sheared by Proteinase K and purified to 350bp sizes. Magnetic beads were used to conduct biotin pull down and tail the adapter. PCR amplification was performed after adapters were ligated to the Hi-C products. The PCR products were purified with AMPure XP beads, and the Hi-C libraries were quantified by qPCR for Illumina HiSeq X-ten PE150 sequencing.