GSM4056931: 202_K_LF_LF: Lean-Low Fat 2; Rattus norvegicus; RNA-Seq
Total RNA was isolated from cardiac tissue using RNeasy Fibrous Tissue Mini Kit (Qiagen). Libraries were prepared using NEBNext rRNA Depletion Kit and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs). The libraries were sequenced and pre-processed by Genome Quebec using an Illumina HiSeq4000 system, with paired-end reads of 100 base pairs and a total of 50 million reads per sample. FASTQ files were aligned and trimmed by Genome Quebec. Sequence alignment was performed using STAR (version 2.5.1b) and the genome assembly Rnor_6.0.
GEO Accession
GSM4056931