GSM4057243: 2 MGT1 mVenus high (in vitro); Homo sapiens; ATAC-seq
ATAC-seq was performed on 20-50,000 (in vivo experiment) or 50-100,000 (in vitro experiment) cells following the standard Illumina Nextera DNA Library Prep tagmentation protocol. Transposition was stopped by addition of Proteinase K and lysing the cell pellets in AL buffer (Qiagen) at 56C for 10min. Tagmented DNA was subsequently purified using AMPure beads. The optimal number of PCR cycles for amplification of tagmented DNA was determined per each sample using 2 μl of template followed by qPCR amplification using heat activated Kappa Hifi polymerase and EvaGreen 1x. Final ATAC-seq library construction was performed in 50 μl qPCR volume and 8-12 μl of template DNA. Illumina compatible primers were previously described elsewhere (Buenrostro et al. 2013).
GEO Accession
GSM4057243