Illumina HiSeq 2000 paired end sequencing; Transcriptome of Escherichia coli O157:H7 (Sakai) in response to spinach leaf lysates by RNA-seq
Transcriptome of Escherichia coli O157:H7 (Sakai) in response to spinach leaf lysates by RNA-seq
E. coli (Sakai) was cultured at 18degC in M9 glycerol and transferred into M9 glycerol (control) or M9 + 40 % (v/v) spinach leaf lysate, and incubated at 1 hr. The samples were collect in RNA later (Qiagen).   Total RNA was extracted using a kit (Qiagen), DNA removed with a DNase (Turbo) and purity and concentration verified on a Bioanalyzer 2100.Ribosomal RNA was depleted with Ribo-Zero kit (Epicentre). Libraries were generated with TruSeq RNA v2 to add Illumina adaptors.
Experimental Factor: growth condition
M9 glycerol