GSM4552088: Noninfected.Rep2.MeRIP; Mus musculus; RIP-Seq
Spleens were removed, flash frozen on dry ice, and RNAs were harvested using Trizol reagent. Total RNA was purified using PolyATtract® mRNA Isolation System IV followed by RiboMinus transcriptome isolation kit for human/mouse. PolyA-selected and ribodepletion-treated mRNA samples were used for RNA fragmentation followed by immunoprecipitation with EpiMark® N6-Methyladenosine Enrichment Kit. Input RNAs and immunoprecipitated RNAs were used for library preparation using TruSeq Stranded mRNA kit. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM4552088