Comparative transcriptome analysis of three domesticated varieties of Auricularia auricula-judae
The total RNAs of Banjin were isolated with TRIzol reagent (Invitrogen, USA) according to the manufacturer's protocols. The quality and quantity of RNA extracts were assessed using agarose gel electrophoresis and a Nanodrop 2000 Spectrophotometer (Thermo Scientific, USA). More than 5 µg of total RNAs were obtained from each of the individual samples with OD260/280 > 1.8 and OD260/230 > 1.8.The cDNA libraries were constructed for each individual sample using the Illumina TruSeq Stranded mRNA Library Preparation Kit LT for mRNA (Illumina, USA). Then, the libraries were sequenced individually using the manufacturer's 125-nt pair-end protocol of the Illumina HiSeq platform at LC Sciences (China)