Pyrosequencing of the seawater sample; the South Sea, 2009
Surface seawater samples (2L) from the South Sea filtered using 0.2 um pore size membrane filter. Total DNA was extracted from the filter and used for amplification of 16S rRNA gene (V1-V3 region; 27f and 519f primers). Amplified DNA was sequenced using 454 GS FLX Titanium platform.