454 pyrosequencing of 16S bacterial diversity of Aphis craccivora collected from black locust.
Bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP) was performed  using universal eubacterial primers Gray28F 5’TTTGATCNTGGCTCAG and Gray519r 5’ GTNTTACNGCGGCKGCTG. Initial generation of the sequencing library used a one-step PCR with a total of 35 cycles, a mixture of Hot Start Plus taq polymerases (Qiagen, Valencia, CA) and amplicons originating and extending from the 28F for bacterial diversity.