RNA-Seq of Mus musculus: striatum
Sample was placed in tubes with 600 l of TriReagent (Merck) and homogenized with the use of Omni Tissue Homogenizer (Omni International). Total RNA was isolated with the use of Direct-zol RNA MiniPrep kit (Zymo Research) according to manufacturers instructions with DNase I treatment. RNA quantity was checked with the use of Qubit RNA BR Assay Kit and Qubit Fluorometer (Thermo Fisher Scientific) according to manufacturers instructions, RNA quality was checked with the use of RNA 6000 Nano Kit and 2100 Bioanalyzer System (Agilent) according to manufacturers instructions. Library preparation and total RNA sequencing (2x100 bp) was performed by CeGaT (Germany) with the use of 100 ng of RNA, TruSeq Stranded Total RNA kit and NovaSeq 6000.