human_22_M
Total RNA was extracted by a Trizol (Invitrogen) procedure according to the manufacturers instructions. All RNA quality was assessed using an Agilent 2100 Bioanalyser.Sequencing libraries were prepared using the Truseq RNA-Seq Sample Prep Kit (Illumina) according to the manufacturers instructions.Polyadenylated RNA was isolated using a poly-dT bead procedure and then chemically fragmented and randomly primed for reverse transcription. After second-strand synthesis, the ends of the double-stranded complementary DNA were repaired. After 3?-end adenylation of these products, Illumina Single-End Sequencing adapters were ligated to the blunt ends of the cDNA fragments. Ligated products were then PCR-amplified (15 cycles). The RNA-seq libraries were sequenced (100 cycles) on the Illumina Hiseq 2000 platform.