UluPandan October RNA Illumina HiSeq 2000 runs
Extracted total RNA was subjected to DNase digestion (Ambion Turbo DNase) to remove trace amounts of DNA prior to RNA library preparation. The DNA-depleted total RNA samples from the October and February samples were subjected to TruSeq RNA Sample Preparation (Illumina), following the manufacturer’s protocol with the following modification: The initial mRNA purification step was skipped and instead, 120 ng of total RNA was reduced to a volume of 5 µL, using a MinElute PCR Purification column (Qiagen). Library preparation with the concentrated total RNA was started at the RNA fragmentation step in the manufacturer’s protocol.