GSM4118690: Control_2 [CC-32]; Mus musculus; RNA-Seq
Single cell suspensions from macrodissected tumours were obtained using a gentleMACS Octo Dissociator (Miltenyi Biotec). RNA from the single cell suspensions was extracted using TRIzol (15596018, ThermoFisher Scientific). TruSeq stranded mRNA kit (Illumina) was used to prepare sequencing libraries, starting from 500 ng total RNA. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM4118690