GSM3602223: DOM_M15_P2; Mus musculus domesticus; RNA-Seq
Pregnant females (n=5/type of pregnancy) were euthanized by cervical dislocation between 10:00 and 11:00 AM on embryonic day 17-18 (e17.5) and the maternal brain was extracted. Embryos were separated from placentas and placentas were weighed, and the maternally-derived decidual layer was removed as previously described (Qu et al. 2014) and discarded.  Only fetally-derived placental tissue was used in this study. All tissues were transferred immediately to RNAlater (Thermo Fisher, USA), kept at 4°C overnight to allow RNAlater perfusion, and stored at -20°C until microdissection and RNA extraction. The maternal MPoA was localized using the Mouse Brain atlas (Figs. 26-33, (Paxinos and Franklin 2013)), and microdissected by sectioning the RNAlater perfused brain at 100μm on a Leica CM 1950 cryostat, followed by dissection under a dissecting microscope in chilled PBS droplets for improved visibility of brain microstructure. Library preparation was performed by the sequencing facility (Novogene, Sacramento, CA), using the NEB Next Ultra RNA library prep kit for Illumina. RNAseq was performed on the Illumina HiSeq 4000 platform, producing >30 million, 150bp paired-end reads per sample.
GEO Accession
GSM3602223