GSM4119733: PLc_1_Sal; Mus musculus; RNA-Seq
Two days following 5 days of ketamine or saline injections, brains were rapidly removed and the PLc was dissected and frozen in isopentane on dry ice. Total RNA was isolated using the TriZol reagent (Invitrogen) and purified using RNeasy micro kits from Qiagen. RNA integrity was assessed using the Agilent Bioanalyser and all RNA Integrity number (RIN) values were above 8. The cDNA library was prepared using the NEB Next Ultra II Library Prep kit (New England Biolabs, USA).
GEO Accession
GSM4119733