GSM1607522: UW240, cKIT+TAB-seq; Homo sapiens; Bisulfite-Seq
DNA was extracted from sorted cells using the Quick-gDNA MiniPrep Kit (Zymo) according to manufacturer's instructions. Bisulfite-Seq: Whole Genome Bisulfite-Seq libraries were genreated using the Ovation Ultralow Methyl-Seq Library Systems (Nugen) according to manufacturers instructions. TAB-seq: TAB-Seq (Tet-assisted bisulfite sequencing) was perfomrmed using the 5hmC TAB-Seq kit from Wisegene followed by the Ovation Ultralow Methyl-Seq Library Systems (Nugen) according to manufacturers instructions.Bisulfite conversion was performed by performing two rounds using the EpiTect Bisulfite Kit (Qiagen) according to manufacturers instructions.
GEO Accession
GSM1607522