Illumina sequencing of Fol4287 single chromosome: RFP#11_SC
We sequenced the putative pathogenicity chromosomes of Fusarium oxysporum from the CHEF gel from the donor and horizontal chromosome transfer (HCT) strains (DOI: 10.1111/1462-2920.15095). we first cut the putative pathogenicity chromosome bands from the gel and isolated DNA from the gel pieces for sequencing. In total, 11 bands from eight strains were sequenced, including band GFP#8_SC from GFP#8, band HCT_GFP#8_SC from HCT_GFP#8, band GFP#26_SC from GFP#26, band HCT_GFP#26_SC from HCT_GFP#26, band RFP#11_SC from RFP#11, band RFP#12_SC from RFP#12, bands GFP#29_SC_L, GFP#29_SC_S, and GFP#29_SC_XS from GFP#29, bands HCT_GFP#29_SC_L and HCT_GFP#29_SC_S from HCT_GFP#29. Most bands were successfully sequenced and contained sequences from the pathogenicity chromosome, as expected.