GSM1608419: No.6 resistant housefly strain; Musca domestica; RNA-Seq
Total RNA from whole bodies of pooled flies (approx. 1.2 g equivalent to 60 flies) was extracted using the RNeasy Maxi Kit (Qiagen). Flies were thoroughly ground with liquid nitrogen, a mortar and pestle and otherwise following the manufacturer’s protocol. Isolated RNA was DNase-treated and concentrated using the RNeasy MinElute Kit (Qiagen). Gel electrophoresis and spectrophotometry (Nanodrop; NanoDrop Technologies, Wilmington, USA) was performed to assess the integrity and the concentration of each RNA sample, which was dissolved in RNase-free water and stored at -20ºC until use. A normalized cDNA library was prepared from 12.2 µg mRNA prepared from adult male and female houseflies. The normalized cDNA library was size fractioned to approx. 500-1,200 bp. High throughput sequencing on GS FLX Titanium of the Musca cDNA library was done according to the standard protocols using a Genome Sequencer FLX Titanium Instrument (Roche Diagnostics). Preparation of cDNA, normalization and sequencing was performed by Eurofins MWG GmbH (Ebersberg, Germany).
GEO Accession
GSM1608419