Genome Sequence of Pectobacterium punjabense SS95
Library preparation and sequencing were performed at the GeT-PlaGe core facility, INRA Toulouse, according to the manufacturers instructions 1D Native barcoding genomic DNA (EXP-NBD103 and SQK-LSK108). At each step, DNA was quantified using the Qubit dsDNA HS Assay Kit (Life Technologies). DNA purity was tested using the nanodrop (Thermofisher) and size distribution and degradation assessed using the Fragment analyzer (AATI) High Sensitivity DNA Fragment Analysis Kit. Purification steps were performed using AMPure XP beads (Beckman Coulter). Five micrograms of DNA was purified then sheared at 20kb using the megaruptor1 system (diagenode). A DNA damage repair step was performed on 3g of sample. Then a END-repair and dA tailing of double stranded DNA fragments were performed on 1g of each sample.  The library was generated and adapters were ligated to the library. Library was loaded on a R9.4.1 flowcell and sequenced on MinION instrument at 0.15 pmol within 48 H