High-throughput SGPV identification: Adult Atlantic salmon visceral organs
A centrifugation, filtration and nuclease-treatment approach was used prior to nucleic acid extraction. Viral nucleic acid extraction was done using the QIAamp UltraSens Virus Kit (Qiagen, MD, USA) and Agencourt Ampure XP Beads (Beckman Coulter, CA, USA), and the library preparation was done using the TruSeq Stranded mRNA Preparation Kit (Illumina, CA, USA)