GSM2125379: YAP; Homo sapiens; RNA-Seq
Total RNAs were purified from  cells  using Trizol method and subsequently cleaned using RNAeasy Kit. Total RNA not exceeding 3 μg was further used to purify polyadenylated RNA using Illumina TruSeq Total RNA Sample Prep kits.  We used the Ribo-Zero Human to remove cytoplasmic rRNA. The mRNA purified was further analyzed using Bioanalyzer (Agilent Technologies) prior to generation of cDNA library with bar coded ends. RNA-seq libraries were robotically prepared with Illumina TruSeq Total RNA Sample Prep kits according to the manufacturer’s protocol.
GEO Accession
GSM2125379