RNA-Seq of Alligator sinensis: adult female hypothalamus
approximately 10g of mixed total RNA were used to remove ribosomal RNA using an Epicentre Ribo-Zero Gold Kit (Illumina, San Diego, CA) according to the manufacturers instructions. Following purification, the poly-A or poly-A+ RNA fractions were fragmented using Fragmentation Buffer under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (50 bp)(Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (50 bp)