GSM3143249: GM6_48_73419; Homo sapiens; RNA-Seq
RNA extractions were performed using the NucleoSpin RNA II kit (Machery-Nagel; Bethlehem, PA) with the DNA digestion step and robotic Qiacube (Qiagen, Valencia, CA) workstation. Purified total RNA was assessed at 260nm and 280nm with a NanoDrop Lite (Thermo Fisher Scientific, Waltham, MA) spectrophotometer. Polyadenylated RNA was purified and used to prepare complementary DNA libraries for high throughput sequencing using the Illumina mRNA-Seq kit according to the manufacturer's protocol (Illumina, San Diego, CA). Sequencing of fragmented complementary cDNA was performed using the Illumina Genome Analyzer Iix
GEO Accession
GSM3143249