Illumina Genome Analyzer IIx paired end sequencing; ChIP-seq NS5 RBPJ
ChIP-seq NS5 RBPJ
NS5 cells were cultured as previously described (Conti et al. 2005), using laminin as a media supplement (20ug/mL), and plated at a density of 28,000/cm2 NS5 cells were fixed sequentially with di(N-succimidyl) glutarate and 1% formaldehyde in phosphate buffered saline and then lysed, sonicated and immunoprecipitated, as previously described (Castro et al., 2011), with rabbit anti-RBPJ DNA libraries were prepared from 10ng of immunoprecipitated DNA according to the standard Illumina ChIP-seq protocol.
Experimental Factor: anti-RBPJ \"RBPSUH\" Cell Signalling #5313
immunoprecipitate