GSM4119220: POS Chronic 10 nM Trametinib Day 1 RNA-seq Replicate 1; Homo sapiens; RNA-Seq
Approximately 1 million cells were rinsed with PBS and scraped from the dish with a cell lifter into 600 ul of RLT Buffer Plus w/ BME. RNA was isolated using the RNeasy Plus RNA isolation kit (Qiagen cat. # 74134), then RNA was converted to cDNA using the High Capacity cDNA Reverse Transcription kit (Applied Biosystems cat. # 4368813). RNA Sequencing library was prepared with 4 mg of total RNA using KAPA stranded mRNA Sequencing kit (Roche cat. # 07962193001) and Illumina TruSeq sample indexes following the manufacturer's protocol.
GEO Accession
GSM4119220