GSM1876243: rdr1/2/6_dry_smRNA rep2; Arabidopsis thaliana; miRNA-Seq
small RNA fraction was extracted from whole plants using Ambion mirVana miRNA Isolation Kit (Life technologies). 10μg small RNA fractions wrere purified into 20-30nt RNA by 8M urea and 7.5% acrylamide gel electrophoresis and gel extraction. 20-30nt small RNAs were constructed to small RNA libraries, using TruSeq Small RNA Library Preparation Kits (Illumina)  The small RNA labraries were read by hi-seq 2000.
GEO Accession
GSM1876243