GSM2473260: rWT H3K4me3 ChIP replicate2; Homo sapiens; ChIP-Seq
Cells were harvested at 72 hours after induction (48 hours after α-amanitin) to perform ChIP using specific antibodies or total RNA, polyA-RNA and Nascent RNA was extracted using corresponding protocols as described in manuscript. ChIP-seq libraries were prepared with Illumina's ChIP Sample Prep Kit according to standard protocol. RNA-seq Libraries were prepared either with Illumina's Small RNA Library Prep Kit by using a modified protocol described in manuscript or with Epicenter's ScriptSeq V2 library preparation protocol according to manufacturer's instructions.
GEO Accession
GSM2473260