UCE target enrichment of Dasyscopelus orientale: muscle tissue
Quantified samples (100 µl volume) were sent to MYcroarray (Ann Arbor, MI) for DNA library prep, target capture (using the MYbaits_Actinopts-UCE-0.5Kv1, a 500 UCE actinopterygian loci probe set; Faircloth, B.C., et al., 2013. PLoS ONE 8: e65923.) and enrichment, sequencing on an Illumina HiSeq 2500, and demultiplexing of samples (MYreads-LibPrep, MYreads-capture, and MYreads-Sequence services).