GSM1607530: Input siHic5; Homo sapiens; ChIP-Seq
Cells were crosslinked with 1% formaldehyde at room temperature for 1 hour, quenched with 0.125M Glycine and washed with PBS. Chromatin was isolated using a lysis buffer containing 1% SDS, 50 mM Tris-HCl (pH 8.0), and 10 mM EDTA. The chromatin extracts were sonicated for 20 min (in repeated cycles of 30-s on/30-s off) using a Bioruptor (Diagenode) kept at 4 °C with a circulating water bath to achieve a DNA fragment size of 300–400 bp. Standard protocol at Next-Generation Sequencing Core of USC Norris Comprehensive Cancer Center
GEO Accession
GSM1607530