GSM4551588: PCNA WT-1; Mus musculus; RNA-Seq
RNA extraction is performed by Direct-zol RNA MiniPrep plus (Zymo Research, USA). Purified RNA was quantified at OD260nm by using a ND-1000 spectrophotometer (Nanodrop Technology,USA), qualitated by using a Bioanalyzer 2100 (Agilent Technology, USA) with RNA 6000 LabChip kit (Agilent Technologies, USA) and sequenced on Illumina NovaSeq 6000 system All RNA sample preparation procedures were carried out to the offical Illumina protocol. Agilent's SureSelect Strand-Specific RNA Library Preparation Kit were used for library construction folllowed by AMPure XP Beads size selection. The sequence was directly determined using Illumina's sequencing-by-synthessis technology. Sequencing data were generated by Welgene's pipeline based on Illumina's basecalling program bc12fastq v2.2.0
GEO Accession
GSM4551588