GSM3603173: WT5: Hb9:Gfp+ wild type 5; Mus musculus; RNA-Seq
Ventral spinal cord regions micro-dissected from E12.5 Arhgap35 (aka, p190) knockout embryos or control littermates (wild-type or p190+/-) were pulled and dissociated with papain (Worthington Biochemical Cat# LK003153) according to the manufacturer's instructions. Cells were sorted on a Becton Dickinson FACS Vantage SE DiVa using Coherent Sapphire 488 nm solid state laser and collected directly into RLT lysis buffer containing β-mercaptoethanol (Qiagen). RNA was isolated using the RNeasy Mini Kit (Qiagen) with on-column DNase digestion (Qiagen RNase-Free DNase Set). RNA samples were quantified with Agilent 2200 TapeStation system prior to preparation of mRNA-sequencing libraries (50 bp single-end) using the Illumina TruSeq RNA Library Preparation Kit (v2) according to the manufacturer's instructions. Libraries were sequenced with Illumina HiSeq 2500 platform. mRNA-Seq libraries were prepared using the TruSeq RNA Library Preparation Kit (v2) (Illumina) according to the manufacturer's instructions.
GEO Accession
GSM3603173