GSM2058529: rN.SLE.4; Homo sapiens; OTHER
Naïve B cells (CD19+IgD+CD3-CD27-Mtg-CD24+CD38-) were sorted by FACS Cells were centrifuged for 10 minutes at 500xg, resuspended in 50 ul of Lysis Buffer (contents), and immediately centrifuged for 30 minutes at 500xg.  Tagmentation was performed using the Illumina Nextera Kit by resuspending cells in 25 ul of Tagmentation mix (1x TD Buffer, 2.5 ul Tn5 Transpoase) and incubating for 60 minutes at 37°C.  DNA was purified and amplified into a sequencing library with KAPA HiFi Polymerase and the Nextera Indexing Kit Primers (Illumina, Inc).
GEO Accession
GSM2058529