ITS1 amplicon metabarcoding of Fungi in Populus trichocarpa leaves
We extracted DNA from approximately 10 mg of lyophilized tissue per leaf using Qiagen DNeasy 96 Plant Kits, following the manufacturer s protocols. We amplified and sequenced fungal ITS1 region for each leaf sample using a modified version of the primers ITS1F and ITS2. PCR products were cleaned and normalized using Just-a-Plate 96 plates. The library was sequenced at the Stanford Functional Genomics Facility on Illumina MiSeq (300-bp paired-end).