16s rRNA sequencing of human stool sample
Stool samples were collected into sterile vials, stored at -20 C after transportation for not longer than 6 hours at +4 C. Further handling was performed with refrigerants preventing thawing. DNA extraction from the stool samples was performed as described previously (Tyakht et al. 2013). Amplification of the V3-V4 regions of 16S rRNA gene was performed. High-throughput sequencing was performed using Illumina MiSeq platform yielding >20000 reads per sample.