GSM2474254: NDH SDC; Sarcophaga bullata; miRNA-Seq
Total RNA was extracted from individual pupae with a mirVana kit (Ambion) according to the manufactuer's instructions.  Concentration was measured with a Nanodrop spectrophotometer. Equal amounts of total RNA from 3 pupae of the same type (SD or LD) was pooled prior to library construction Illumina Truseq Small RNA Preparation kit according to the sample preparation guide.
GEO Accession
GSM2474254