16S rRNA amplicon sequencing of groundwater bioreactor microbiome
A two-step PCR for library prep: 1) primers 515F [5-GTGCCAGCMGCCGCGGTAA-3] and 806R [5-GGACTACHVGGGTWT CTAAT-3] to amplify the V4 region of prokaryotic 16S rRNA genes 2) Phasing primers, designed with 0-7 base-length spacers to increase base diversity, containing barcodes for sample identification