GSM4551487: undifferentiated_LaminB1-Dam; Mus musculus; OTHER
Lentivirus encoding the control Dam, or LaminB1-Dam fusion, was used to transduce untreated preadipocytes or differentiating adipocytes at day 6 post-induction. Transduction was allowed for 36h. At day 9 post-induction genomic DNA was extracted. Genomic DNA was extracted using Qiagen DNA-Easy kit, following manufacturer's instructions. Dam-methylated genomic DNA was enriched using the standard protocol as described by Vogel et al. (2007) Nature Protocols 2:1467-1478. It involves digesting gDNA with DpnI enzyme, which cuts at non-Adenine-methylated GATC motifs. Libraries were prepared by Beijing Genomics Institute (BGI) to Illumina's specifications
GEO Accession
GSM4551487