GSM4669149: d7344.PD (PD rep4); Mus musculus; RNA-Seq
For RNA isolation, 10,000 HSCs in G0/1 were isolated in the absence of FBS and HSCs were sorted into 750 μL TrizolLS. During the sort, the collection  sample was vortexed every 10 min. Post-sort, the samples were brought to a total volume of 1 mL  with DEPC treated water. Samples were stored at -80 °C or directly used for RNA extraction. To  extract RNA, 200 μL chloroform was added to the sample and briefly vortexed at medium speed.  After 2 min at RT, the sample was centrifuged at 12,000 x g for 15 min at 4 °C. 400 μL of aqueous  phase was removed and put into 1,400 μL RLT buffer (Qiagen) with 14 μL β-mercaptoethanol and  mixed vigorously. 1 mL 100 % ethanol was added to each sample, mixed by pipetting, then loaded  onto RNeasy Micro Kit (Qiagen) columns. Clontech v4 Low-Input RNA Kit
GEO Accession
GSM4669149