GSM3143951: 001_7_5_WW1_N727S507; Mus musculus; RNA-Seq
Sorted cells were lysed in Trizol LS (ThermoFisher), RNA was prepared using miRNeasy micro kit from Qiagen and quantified using real-time PCR Adapted from the Smart-seq2 protocol, an oligo-based capture of Poly-A tailed mRNA was carried out followed by a high-fidelity reverse transcription using locked nucleic acids (LNA)-oligos to obtain full-length transcripts. mRNA complementary (c)DNA strands were then amplified using a PCR-based method and sequencing adaptors incorporated into the cDNA fragments in a single step using Tn5 transposase technology (Nextera XT reagents and barcoded adaptors kits).
GEO Accession
GSM3143951