200bp insert size Illumina pair-end transcriptome sequencing
Illumina pair-end sequencing for 200bp insert size transcriptome library. Designed lane number: 1, cycle number: 99
Poly-A RNA was isolated from total RNA. First strand cDNA synthesis was performed with random hexamers and reverse transcriptase.                  The second strand was synthesized with E. coli DNA PolI. Double stranded cDNA was purified nd sheared with a nebulizer (Invitrogen) to 100-500bp fragments.                  After end repair and addition of a 3’dA overhang the cDNA was ligated to Illumina PE adapter oligo mix, and size selected to 200±20 bp fragments by gel purification