Whole genome sequence of pooled females to develop SNP markers for Cotesia vestalis
In order to build a draft reference genome and to develop SNP assays, we sequenced the entire genome of C. vestalis on a single lane of paired-end sequences (2x100 bp) on an Illumina HiSeq 2000 (Illumina Inc., U.S.A.) instrument. The SNP discovery panel consisted of eight C. vestalis females, one from each of eight different cabbage fields in Western Taiwan. DNA from the entire bodies was extracted as a pool using Qiagen’s DNeasy kit following the protocol for tissue DNA with an extended incubation time in buffer ATL (3 h at 56°C). A single genomic library with an insert size of approximately 300 bp was prepared using the Illumina Sample Preparation Kit and sequenced for 100 cycles with the Illumina HiSeq 2000 (paired-end) at the UMCG Sequencing Facility in Groningen, The Netherlands.