GSM4669816: Epitope-decreased; Mus musculus; RNA-Seq
RNA was extracted from sorted cells by RNeasy Micro Kit (Qiagen) and the quality of RNA was assessed by Nanodrop and quantified by Qubit Fluorometer. RNA was reverse transcribed into cDNA by 10× SMARTScribe Reverse Transcriptase (Clontech). The cDNA was purified using a MinElute PCR Purification Kit (Qiagen). The RNA-seq library preparation was executed by NEBNext Ultra II DNA Library Prep kit following manufacturer's instruction.
GEO Accession
GSM4669816