GSM2476049: seed following 48 h of stratification (S) Replicate 1 [methylC-seq]; Arabidopsis thaliana; Bisulfite-Seq
gDNA was extracted from the seeds/seedlings using the Qiagen DNeasy Plant minikit and 600ng of purified gDNA was used for MethylC-seq library preparation after spiking in 0.5% lambda DNA (N6-methyladenine-free) (New England BioLabs). MethylC-seq library preparation was carried out as described previously (Urich et al., 2015 - http://www.nature.com/nprot/journal/v10/n3/full/nprot.2014.114.html).
GEO Accession
GSM2476049