Metatranscritomics analysis of human gut microbiota
Four subjects get a low fiber diet (10g fiber per days)  during five days and after a washout period a 15 days, they get a rich fiber (40g fiber per days) diet during five days. From fecal sample obtained at the end point of the two diet period (2 fecal samples by subject), RNA were extracted, rRNA removal were done and cDNA libraries were contructed with the whole transcriptomics kit (Sigma).
cDNA libraries were contructed with the whole transcriptomics kit (Sigma).