RNA-seq of Citrullus lanatus: flesh infected by cucumber green mottle mosaic virus
Purified poly (A) +mRNA was extracted from total RNA sample using Oligo (dT) magnetic beads. The mRNA was fragmented into about 200bp pieces by adding a fragmentation buffer. The first-strand cDNA was synthesized using SuperScript II reverse transcriptase and random hexamer primers. After second-strand cDNA generated, the double-strand cDNA was end-repaired, a single ĄŽAĄŻ base and indexed adapters were ligated to the fragments. The cDNA library was constructed and then was sequenced using Illumina HiSeq2000 platform.