Genomic sequencing of population of C. nouraguensis strain NIC59 worms
A large population of worms (thousands) was collected and frozen in a conical tube. Worms were thawed and lysed in lysis buffer containing proteinase K. Samples were treated with RNAse A and protein was precipitated out of the worm lysate, removed, then DNA was precipitated out. The DNA pellet was resuspended in 10 mM Tris-HCl (pH 8) and remaining traces of protein were removed using phenol:chloroform extractions and DNA was precipitated again. The DNA pellet was then resuspended in 10 mM Tris-HCl (pH 8).