GSM4669105: D18-270005: AOX1.24G rep2; Komagataella phaffii; ATAC-seq
Two million cells per sample were prepared for transposition as described previously (doi:10.1101/gr.192294.115). Spheroplasts were washed once with transposition buffer prior to transposition and PCR amplification for sequencing, which are described elsewhere (doi:10.1002/0471142727.mb2129s109). Amplified libraries were size selected using 0.4x followed by 0.8x Ampure XP beads according to manufacturer instructions (Beckman Coulter). Size-selected libraries were sequenced on an Illumina NextSeq to generate 40-nt paired-end reads.
GEO Accession
GSM4669105