Genome sequence of Pseudomonas aeruginosa ST664 clinical isolate
Library preparation was performed according to Illuminas TruSeq Nano DNA Sample Preparation protocol. The samples were sheared on a Covaris E220 to ~550bp, following the manufacturers recommendation, and uniquely tagged with one of Illuminas TruSeq LT DNA barcodes to enable sample pooling for sequencing. Finished libraries were quantitated using Promega's QuantiFluor dsDNA assay and the average library size was determined on an Agilent Tapestation 4200. Library concentrations were then normalized to 4nM and validated by qPCR on a QuantStudio-3 real-time PCR system (Applied Biosystems), using the Kapa library quantification kit for Illumina platforms (Kapa Biosystems). The libraries were then pooled at equimolar concentrations and sequenced on the Illumina MiSeq platform at a read-length of 300bp paired-end.