Ocean Station Papa seawater plusFe incubation - SOLiD
RNA was fragmented enzymatically using RNAse III according to the SOLiD protocol.  After achieving the desired average fragment size of 100-200 bp, the mRNA was hybridized and SOLiD specific adaptors were ligated to the ends of the mRNA. The mRNA was then reverse transcribed to cDNA and run on a Novex 6% TBE-Urea gel 1.0mM (Invitrogen). Size selection of the cDNA was performed by cutting out the 100bp-200bp region from the gel.  cDNA was amplified in the gel for 15 cycles according to the SOLiD protocol. Amplified cDNA was purified and concentrated using the PureLink Micro Kit (Invitrogen).  Each sample was diluted to 60 pg/ul, and a single emulsion PCR was performed for each sample according to the SOLiD protocol.