GSM4418993: C57BL/6 WT tibialis anterior 30 months 10X snRNA-seq; Mus musculus; RNA-Seq
Nuclei were isolated from skeletal muscle immediately following euthanasia. Muscle was homogenized and then filtered, and nuclei were purified from the homogenate by staining with Hoechst dye and then FACS sorting for DAPI+ nuclei. Isolated nuclei were loaded into the 10X Chromium system (targeting ~12,000-13,000 loading count) using the Single Cell 3' Reagent Kit v3, and libraries were constructed according to the manufacturer's protocol.
GEO Accession
GSM4418993