Composition of fecal microbiota from Akkermansia muciniphila fed mice3
The amplified products were purified with the CleanPCR. Equal concentrations of purified products were pooled, and short fragments (non-target products) were removed using CleanPCR. The quality and product size were assessed using a Bioanalyzer 2100 with a DNA 7500 chip. Mixed amplicons were sequenced with Illumina MiSeq Sequencing system.