GSM4551441: C5; Mus musculus; Bisulfite-Seq
Sperm was lysed in lysis buffer (7.3 mM Tris-HCl (pH 7.5), 7.3 mM NaCl, 18.2 mM EDTA, 1.8% SDS, 1.8% 2-ME, 1.4 mg/ml proteinase K) and DNA was extracted with a phenol-chloroform mixture, precipitated with ethanol, dried and dissolved in 10 mM Tris (pH7.4). RRBS libraries were prepared as previously described (Matsushita et al.,BMC cancer 18: 317, 2018; Okamura et al. Cancer Sci 110:2629, 2019) with some modifications. Briefly, 100 ng of genomic DNA was sonicated for one second by a Focused-ultrasonicator (Covaris) before restriction enzyme reaction.
GEO Accession
GSM4551441