GSM1608514: GM12878_Rad21_2.1; Homo sapiens; ChIA-PET
Cells for GM12878 were crosslinked and chromatin prepared for immunoprecipitation with antibodies against Rad21 and H3K4me3, respectively. Immuno-complexes were pulled down with Protein-G dynabeads, marked with biotin and proximity ligated. Biotinylated fragments were pulled down with Streptavidin Dynabeads and libraries prepared for Illumina sequencing. For ChIP-Seq, nuclear lysates were sonicated using a Branson 250 Sonifier (power setting 2, 100% duty cycle for 7 x 30-s intervals). Clarified lysates corresponding to 20 million cells were treated with 1-5ug of antibody coupled to Protein G Dynabeads (Invitrogen #10003D, New York). The protein-DNA complexes were washed with RIPA buffer and eluted in 1% SDS TE at 65°C. ChIP DNA sequencing libraries were generated according to Illumina DNA Tru-Seq DNA Sample Preparation Kit Instructions (Illumina Part # FC-121-2001, San Diego, CA).
GEO Accession
GSM1608514