Project to sequence Propionibacterium acidipropionici ATCC 55737
DNA was extracted using PureLink genomic DNA mini kit (Invitrogen Cat. No. K1820-01) and quantified using Nanodrop 1000 (Thermo Scientific) and Qubit dsDNA BR assay kit (Life Technologies Cat. No. Q32850). The quality of the DNA was determined by running a 1% agarose gel with DNA gel stain SYBR safe (Life Technologies Cat. No. S33102). The gel was visualized in a ChemiDoc MP system (Bio-Rad). Genome closure was achieved using the PacBio RS II platform for sequencing. The PacBio library preparation was performed using the protocol for 20 Kb selected with the BluePippin system. The chemistry used for sequencing was the release P6-C4. The sample was loaded using magnetic beads. The genome assembly was performed with the SMRT portal.