Illumina sequencing of Turicibacter sp MMM721
After 48 hrs of growth, total genomic DNA was extracted using the PureLink genomic DNA extraction minikit (Life Technologies, Carlsbad, CA), following the manufactures instructions. The quality of extracted DNA was assessed using a Qubit fluorimeter (dsDNA BR kit; Life Technologies), NanoDrop (Thermo Fisher Scientific, Waltham, MA), and gel electrophoresis. A Nextera Flex barcoding kit (Illumina, San Diego, CA) was used to prepare a genomic library for Illumina MiSeq sequencing, according to the manufacturers instructions, and sequencing was carried out on an Illumina MiSeq instrument (Illumina).