GSM3143834: White.Day14.R4; Theobroma cacao; RNA-Seq
Four replicates were sampled at each time point (d0, d1, d7, d8 and d14) from the W-B and D treatments for total of 36 samples. Total RNA was extracted from each cell suspension taking 0.1mL approximately of pellet volume using combined protocols of PureLink Plant RNA Reagent (Ref: 12322012, Thermo Fisher Scientific) and RNeasy Plus Universal Mini Kit (Cat No. 73404, QIAGEN) following the manufacturer's protocol. RNA concentrations and quality were measured using an Agilent 2100 bioanalyzer. The construction of the cDNA libraries and RNAseq were performed by the Genomics Core Facility at Penn State University (University Park, USA). cDNA libraries were prepared with a TruSeq stranded mRNA library prep Kit (cat# RS-122-2101, Illumina, San Diego, CA, USA). The libraries were sequenced on a HiSeq™ 2500 (Illumina) using single-end runs of 100 nt. Library construction was done following the TruSeq® Stranded mRNA Sample Preparation Guide by Illumina available in https://support.illumina.com/downloads/truseq_stranded_mrna_sample_preparation_guide_15031047.html
GEO Accession
GSM3143834