GSM2475210: 4C.Hoxc.Hind3.WT.rep2; Mus musculus; OTHER
10 million cells were trypsinized and crosslinked in 2% formaldehyde for 10 min at room  temperature. First digestion was performed using HinDIII  restriction enzyme. After ligation and crosslink reversal, second digestion was performed using DpnII restriction enzyme. DNA was purified for 4C library preparation. Following DNA purification, 1mg of total DNA was amplified for 30 cycles using 4C primer sets. Fragments of 100-700bp were selected using agarose gel extraction. Libraries were quantified and pooled. 4C-seq
GEO Accession
GSM2475210