Metagenomic RNASeq of cerebral spinal fluid, treated with DASH, a Cas9 based method that removes unwanted high abundance sequences, for the purposes of increasing sensitivity of NGS based diagnostics for infectious disease.: Sample 1924_D
Amplified cDNA derived from cerebral spinal fluid was produced using the NuGen Ovation v2 kit, followed tagmentation using Nextera (Illumina). For removal of unwanted high abundance species, cDNA library was treated with Cas9-RNPs programmed with selected sgRNAs, such as the the mitochondrial rRNAs. 