PDP4
Dental plaque of 3-4 sites was sampled from one periodontitis patient and pooled together to get adequate yields of microbial DNA for WGS sequencing. Metagenomic DNA was isolated from this sample, quantified using a Qubit Fluorometer (Invitrogen) before sheared into about 180 bp fragments with a Covaris Focused-ultrasonicator (Covaris). Libraries were prepared on the sheared fragments using a TruSeq Sample Preparation Kit v2 (Illumina) according to the manufacturer's instructions. Fragment size and integrity of the libraries were assessed by a Fragment Analyzer (Advanced Analytical Technologies). Libraries were sequenced with 2×101 bp paired-end (PE) on an Illumina HiSeq 2000 platform.