Phylotranscriptomics of Heliozelidae (Adeloidea, Lepidoptera)
RNA was extracted from specimens using the Nucleospin RNA XS (Macherey-Nagel) kit following the protocol for small samples, and RNA quality was checked using an Agilent 2200 TapeStation (Agilent Technologies). RNA libraries were prepared with the TruSeq mRNA library protocol for low inputs in half the standard reaction volume (25 uL) and quality checked using the TapeStation. Samples were multiplexed and sequenced on the Illumina NextSeq or MiSeq platform using paired end reads.