GSM3142536: DGRP-551_15d_r2_SDP; Drosophila melanogaster; RNA-Seq
Adult flies were anesthetised by placing them in ice cold glass dishes, heads were removed and brains were dissected removing any cuticle, retina and trachea. Dissected brains were placed in ice cold PBS. When 40 brains were collected, brains were dissociated at 25'C for 15 hours in a mix of Collagenase (75µl @ 100mg/ml), Dispase (50µl @ 3mg/ml) and Trypsin 0.05% EDTA (125µl), the sample was pipette mixed every 5 minutes. Cells washed in PBS twice centrifuging at 800 xg) and resuspended in 400µl PBS. A single cell suspension was obtained by filtering with an 11µm cell strainer. Drop-seq and 10x Genomics CHROMIUM Single Cell 3' Solution V2 Chemistry
GEO Accession
GSM3142536