RNA-seq of buckwheat hypocotyls: adventitious roots formation
The explants were placed in medium with IAA (3 M) and were collected 6 h after the addition of effectors. The samples were fixed with liquid nitrogen and stored at 80C until RNA extraction. The total RNA from collected sample was extracted using a TRIzol analogue combined with an RNeasy Mini Kit (Qiagen, Hilden, Germany). Polysaccharides and polyphenolics were removed from the RNA preparations by adding the Plant RNA Isolation Aid reagent (Ambion, USA) and -mercaptoethanol to the lysis buffer RLT. The preparation of cDNA library was performed using the TruSeq Sample Prep Kit v3 (Illumina, USA).