Garden City, September 2017: Suptratidal 10cm
Genomic DNA was isolated from 0.50-0.75 g of each sample of sand using the DNeasy PowerLyzer PowerSoil Kit (QIAGEN; Hilden, Germany) according to the manufacturers instruction. DNA samples were diluted to 1 ng l-1 and prepared for sequencing using a barcoded 16Sf/16Sr primer set targeting the V4 region of the 16S rRNA subunit according to Kozich et al. [31]. Sequencing was performed on a MiSeq V2 2x250bp Illumina platform (San Diego, CA, USA) at Clemson University.