GSM4669427: Chronic hypertension Patient 1, 1st Trimester (WGBS); Homo sapiens;    Bisulfite-Seq
Peripheral venous blood was collected into      EDTA-coated tubes. Blood was then centrifuged at 2000 X g for 7 minutes at 4°C,      in order to collect only the plasma fraction. Plasma was centrifuged at 16000 X      g for 10 min at 4°C to remove all the residual cells, aliquoted and stored at      -80°C. Cell-free DNA was extracted from 200 µL of plasma using the QIAmp      MiniElute Virus Spin kit (Qiagen). Total DNA was quantified using the Qubit      dsDNA HS Assay kit (Thermofisher Scientific). Whole genome bisulfite libraries      were prepared using the pico methyl-Seq library prep kit (Zymo Research)      according to manufacturer's instructions. Libraries were sequenced using 100 bp      single-end reads on the Hiseq4000 System      (Illumina)