GSM3682505: mRNA seq from PancB1Q; Homo sapiens; RNA-Seq
Cell culture medium was removed, cells were washed with DPBS, TRIzol (Life Technologies) was added and cells were processed according to manufacturer's guidelines. Up to 4 µg of total RNA was used as an input for the Stranded mRNA-Seq Kit (KAPA). mRNA is captured using magnetic oligo-dT beads: while mRNA is retained, other RNA is washed away in two subsequent washes. mRNA fragments are fragmented and converted to cDNA, with dUTP incorporated in to the second cDNA strands. A-tailing, adapter ligation and enrichment is carried out, to obtain libraries. Enrichment of the libraries is achieved by using KAPA HiFi Hotstart Readymix. The strand marked with dUTP is not amplified, allowing strand specificity.
GEO Accession
GSM3682505