GSM1876036: HCASMC_ChIP_H3K27ac_D1; Homo sapiens; ChIP-Seq
1x10^7 HCASMC per condition were crosslinked for 10 min with 1% formaldehyde, followed by quenching with glycine. Cells were lysed for 10 min on ice, and nuclei were then lysed for 10 min on ice. Crosslinked chromatin was sheared to 100-500bp fragments by sonication for 3x5 min using a Bioruptor Pico sonicator (Diagenode). 5 ug IgG or H3K27ac (Abcam ab4729) antibody was incubated with diluted chromatin overnight at 4C, and ChIP DNA was receoved as previously described. Libraries were generated by ligating ChIP DNA with Illumina TruSeq adapters, followed by PCR amplification and gel electrophoresis. ChIP DNA library fragments around 300bp were selected for PCR amplification and libraries were quantitated using Qubit fluorometric and bioanalyzer analyses.
GEO Accession
GSM1876036