NextSeq 500 paired end sequencing; ATAC-seq of human Barrett's oesophagus tissue
ATAC-seq of human Barrett's oesophagus tissue
Barrett's oesophagus biopsies were collected from routine endoscopies and placed into PBS on ice. Biopsies were minced with a scalpel and lysed in ATAC lysis buffer and Tn5 reactions were performed as described in Transposition of native chromatin for fast and sensitive epigenomic profiling of open chromatin, DNA-binding proteins and nucleosome position. 2013. Jason D. Buenrostro, Paul G. Giresi, Lisa C. Zaba, Howard Y. Chang and William J. Greenleaf. doi:10.1038/nmeth.2688  Libraries were constructed by amplifying transposed DNA with Illumina Nextera adapters
Experimental Factor: individual
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