GSM4419070: d1_RXRi_prep2_2; Mus musculus; RNA-Seq
RNA was isolated using Single Cell RNA Purification Kit (Norgen Biotek Corporation, Cat #51800) pooling 3 wells of 24 well plates (C57BL/6 wild type). Organoids were seeded as single cells at 50000 cells per well and cultured as described above. RNA purification was performed in duplicate for two organoid cultures (C57BL/6 wild type, male littermate mice) for all treatment conditions. A step of DNAse treatment was included (RNase-Free DNase I Kit, Cat #25710) for all samples. RNA sequencing libraries were prepared using the TruSeq Illumina mRNA Library Prep
GEO Accession
GSM4419070