GSM2475825: Type2 neuroblast 24hr after larva hatch replicate 3; Drosophila melanogaster; RNA-Seq
30 fly larvae brain tissues were dissected at defined ages, digested with a mixture of pronase (1mg/ml) and dispase (1mg/ml) for 30 min at 25C, washed, triturated and 10 GFP labeled cells were sorted with a robotic sorter and stored at -80C in XB buffer from PicoPure kit (Life Technologies, KIT0204). Total RNA from around 100 pooled cells was extracted using PicoPure kit. Extracted RNA was amplified using Ovation RNA-Seq System V2 (NuGEN, #7102). 1~1.5 ug of the amplified material was used for library construction using Encore NGS Multiplex System (NuGEN, #0314).
GEO Accession
GSM2475825