Fungal ITS amplicon sequencing of gut metagenome: sample Trap3
Fungal ITS amplicon library was created using a barcoded fusion primer PCR protocol using Pfx50 polymerase (Thermo Fisher Scientific), size selected, and sequenced on the Ion Torrent PGM platform.  Sequence data was minimally processed, sequences were exported directly from the sequencing platform and only the barcode (index sequence) was trimmed prior to SRA submission.