GSM3603045: Control_pEUC1-EUC1_Exp2_Rep3; Saccharomyces cerevisiae; RNA-Seq
Yeast total RNA was isolated form 4 OD600 units of yeast cells using the RNeasy Kit (Qiagen) following the manufacturer's instruction, with beat-beating for cell lysis and DNase I digest performed on-column. polyA RNA was enriched (NEBNext Poly(A) mRNA Magnetic Isolation Module #E7490), libraries were prepared for sequencing (NEBNext Ultra II RNA library prep Set for Illumina #E7770L) and barcoded (NEBNext Multiplex Oligo for Illumina #E7335L, #E7500L, #E7710L, #E7730L), all according to the manufacturer's instructions.
GEO Accession
GSM3603045