GSM2652693: Forsubtelomere_YXY1007_Input_sir3del_replicate2; Saccharomyces cerevisiae; ChIP-Seq
Cell were lysed with glass beads in lysis buffer  and chromatin were extensively washed. chromatin was sonicated for 4 minutes with 30s on and 1 min off on a Biodisruptor. The supernatant from sonicated lysates were precleared with Protein A beads and ChIP was performed as described using commercial antibodies Cell were lysed with glass beads in lysis buffer  and chromatin were extensively washed. chromatin was sonicated for 4 minutes with 30s on and 1 min off on a Biodisruptor. The supernatant from sonicated lysates were precleared with Protein A/G beads and ChIP was performed as described using commercial antibodies Libraries were prepared with a KAPA LTP kit following the manufacturer's protocol
GEO Accession
GSM2652693