16S sequencing of mouse fecal microbiota of mice fed high fat diet in the context of different amounts and sources of calcium
Bacterial tag encoded FLX-Titanium amplicon pyrosequencing (bTEFAP)was performed using a Genome Sequencer FLX System (Roche Diagnostics, Indianapolis, IN).   The variable region V1 to V3 region of 16S was sequenced using the primer pair- 27F-519R.