GSM3143435: HaCaT_WGBS_rep_3; Homo sapiens; Bisulfite-Seq
Chromatin was isolated from K562 cells that were cross-linked using 1% formaldehyde for 10 min at RT and prepared using a ChIP-qPCR Kit (Chromatrap, cat. no. 500117). Chromatin was sonicated using a Bioruptor Plus (Diagenode) to give a fragment distribution of 100-500 bp. WGBS was performed using Pico Methyl-seq kit from Zymo Research. G4 ChIPed DNA was subjected to Nextera library preparation (Illumina, cat. no. FC-121-1030). For further details see methods section in the associated manuscript.
GEO Accession
GSM3143435