RNA-seq of purslane: leave and root
Disinfected RNA was isolated from total RNA using oligo (dT) enriches mRNA containing poly(A) beads. The SMART PCR cDNA Synthesis Kit were used for cDNA synthesis. For the selection of differently sized full-length cDNA and for libraries building cDNA the BluePippin were used. After screened by Blue Pippin the fragments were subjected to large-scale PCR to obtain sufficient total cDNA by using Qubit fluorometer. The libraries quality was sustained by using Agilent Bioanalyzer 2100 system and SMRT sequencing was achieved via Pacific Biosciences real-time sequencer using C2 sequencing reagents.