GSM2817854: UF_KS_041_S5_L001_R1_001; Homo sapiens; miRNA-Seq
Total RNA was extracted using the miRNeasy Mini Kit (Qiagen) according to the manufacturer's protocol. The cDNA and the final sequencing libraries were prepared as described before (Wang et.al, EMBO Mol Med, 2013). It has to be noted that the resulting double-stranded cDNA fragments do not preserve information about the strand specificity of the original transcript. The libraries were sequenced using the Illumina HiSeq 2000 for 1 x 51cycles following the standard protocol. According to the protocol of TruSeq Small RNA kit manufacturer
GEO Accession
GSM2817854