GSM4217491: IRBB5_24h_2; Oryza sativa Japonica Group; miRNA-Seq
Every sample was pooled with infected leaves collected from twenty plants in one plot to diminish the individual environment differences.Total RNA were extracted from the mentioned samples using Trizol reagent (Invitrogen, USA) according to the manufacturer's recommendation. Small RNAs with 18~30 nt in length were isolated from the total RNA using a polyacrylamide gel electrophoresis gel, and then were sequenced by the BGISEQ-500.
GEO Accession
GSM4217491