GSM2817915: Ton185 CD45RA+CD8+; Homo sapiens; RNA-Seq
Total RNA from FACS-sorted CD45RA+CD8+, CXCR5-CD45RA-CD8+, and CXCR5+CD45RA-CD8+ T cells from human tonsil samples were extracted using Trizol, followed by purification using NucleoSpin RNA XS kit (CloneTech). The RNA purity was determined by Bioanalyzer 2100 traces (Agilent Technologies). RNA was amplified using the SMARTer® Universal Low Input RNA Kit. The RNA library was prepared following the protocol of TruSeq® Stranded Total RNA Sample Preparation from Illumina. Pair-end reads of 100 nt (2x100 nt) were obtained on Illumina HiSeq 2500.
GEO Accession
GSM2817915