GSM1611603: RNA130926RH_1; Mus musculus; RNA-Seq
Brain nucleus accumbens shell was dissected via laser microdissection. Total RNA was extracted by using acturus picopure RNA extraction columns. RNA libraries were prepared for sequencing using TruSeq PolyA Illumina protocol: PolyA(+) RNA was recovered from 1 ug of total RNA using oligo-dT attached to magnetic beads. PolyA(+) RNA was chemically fragmented. Double stranded DNA was made from the fragmented RNA using random hexamers. All ends were repaired.  A single A was added to each of the 3’ ends of each fragment. Illumina adaptors were ligated to the fragments.  Concentration was determined by real time PCR. Samples were mixed for multiplexing. Samples were diluted to target 180 million templates per lane on the flow cell
GEO Accession
GSM1611603