G. polyphemus OLD107 - immunome sequencing
We created Illumina adaptor-ligated libraries using Bioo Scientific Nextflex Pre- and Post- Capture Combo Kit- Set A (catalog no. 5144-51, Bioo Scientific Corp., Austin, TX, USA), pooled 16 prepared libraries per capture reaction, and used Bioo Scientific reagents and protocols for sequence capture. We then sequenced post-capture amplification libraries using two standard Illumina MiSeq sequencer flow cell using 75-bp paired-end reads (i.e., each library sequenced twice).