GSM1689667: repB_TP3; Plasmodium falciparum; OTHER
Total RNA was isolated as previously reported (Kyes at al, 2000). RNA samples were then treated with Terminator 5‘-Phosphate-Dependent Exonuclease. rRNA-depleted RNA was treated with of Calf Intestinal Alkaline Phosphatase (New England BioLabs). CIP-treated RNA was then incubated with Tobacco Acid Pyrophosphatase andincubated overnight with RNA adapters and T4 RNA ligase. RNA was chemically fragmented and reverse transcribed. cDNA second strand was synthesised using biotinylated primers. DNA libraries were constructed using the standard Illumina protocol and amplified with the KAPA HiFi HotStart ReadyMix.
GEO Accession
GSM1689667