PacBio Genome Sequencing of Sphingobium herbicidovornas MH
10 g HMW DNA from strain MH was diluted in 150 l and sheared on a Covaris g-tube spinning 2 1min at 4,600rpm on an Eppendorf 5424, targeting an insert size of 1520kb. Sheared DNA was size selected on a Sage Pippin with an S1 ladder and using a 15- to 20-kb v3 protocol. PacBio protocol for 10-kb inserts was used and ligation run overnight. Final library was loaded on two SMRTcells with either 0.02 or 0.04nM on plate concentration.