RNA-seq from two kinds of Ae. tauschii??IG47182?
Total RNA was isolated from leaves of 21-d-old seedlings using RNeasy Plant Mini Kit (QIAGEN). mRNA was isolated from 45?g of RNA using mRNA Purification Kit (Takara). 200ng of mRNA was used to fragment mRNA and synthesize cDNA using cDNA Synthesis System (Roche). Approximately 1000,000,000 molecules of the adapter-ligated cDNA samples were used for library preparation using GS FLX Titanium Rapid Library Preparation Kit (Roche). The library was sequenced with GS FLX Titanium Sequencing Kit on GS FLX System (Roche Diagnostics, Mannheim, Germany) according to manufacturer?s instruction.
Submitters
Kazuhiro Sato