WGS of Plasmodium falciparum:  control sample, replicate 2
PCR-free  whole-genome Illumina sequencing. Genomic DNA was sheared to ~150-400 bp  fragments using a Covaris S220 ultrasonicator and analysed using an Agilent  2100 Bioanalyzer. For library preparation we used the NEBNext DNA Library  Prep Master Mix Set for Illumina (no. E6040S) using specific paired-end  TruSeq Illumina adaptors for each sample. We obtained >6 million 150 bp paired reads for each sample using an Illumina MiSeq sequencing system. Sequence reads were mapped to the PlasmodDB P. falciparum 3D7 reference genome version 24 (https://plasmodb.org/common/downloads/release-24/Pfalciparum3D7/) using the Bowtie2 local alignment algorithm.