GSM2652242: Bulk sequencing of trophospheres stimulated with Bmp4 / Nodal NR-bulkrescue7; Mus musculus; RNA-Seq
Cells were directly placed into Trizol (Life Technologies) and total RNA was isolated according to the manufacturer’s instructions, with the following alterations. RNA was precipitated overnight at -20 °C, with 2.5µg GlycoBlue (Life Technologies). RNA was processed using the previously described CEL-seq technique, with the indicated modifications. Libraries were sequenced on an Illumina NextSeq500 using 75bp paired end sequencing.
GEO Accession
GSM2652242