GSM1692825: Genomic DNA digested with MNase rep1; Mus musculus; OTHER
Genomic DNA was treated with MNase. The reaction was quenched by addition of EDTA to 16 mM. The sample was digested with proteinase K, was extracted with phenol and chloroform, and was precipitated with ethanol. DNA was end-repaired using End-It DNA End-Repair Kit (Epicenter) and 3' ends were adenylated by treating the samples with Klenow (exo-) (NEB) in the presence of dATP.  The samples were then ligated to Illumina TruSeq adapters. The ligated products were run on agarose gel and were purified using Qiagen MinElute columns. The ligated products were then PCR-amplified using KAPA master mix. The amplified products were run on agarose gel and were purified using Qiagen MinElute columns.
GEO Accession
GSM1692825