GSM3839842: Oxy_Mut_MNase-seq_naked_DNA; Oxytricha trifallax; MNase-Seq
Naked MNase-digested DNA. MNase digest protocol: Native (methylated) or PCR-amplified (no methyl) gDNA was digested with MNase to approximately mononucleosome-sized fragments. Note that naked DNA was used: no histones were present. Mononucleosome-sized DNA fragments were gel-purified using a QIAquick gel extraction kit (QIAGEN) for subsequent paired-end sequencing on an Illumina HiSeq 2500 according to manufacturer's instructions. Naked gDNA-seq (Paired-end sequencing); standard TruSeq Illumina library construction protocol
GEO Accession
GSM3839842