Genome survey of RNA viruses within fish liver
Following DNase I digestion, total RNA was subjected to an rRNA removal step using Ribo-Zero™ Magnetic Gold Kit (Epidemiology). The remaining RNA was then fragmented, reverse-transcribed, ends repaired, dA-tailed, adaptor ligated, purified, and quantified with Agilent 2100 Bioanalyzer and ABI StepOnePlus Real-Time PCR System.