GSM2128414: wdr12: GFP- rep1; Oryzias latipes; RNA-Seq
RNA isolation was performed using the PicoPureTM Isolation kit according to the manufacturer's protocol. Total RNA concentration and quality were checked in an Agilent Bioanalyser. 4ng of RNA were used for each library construction (Epicentre). Library construction was performed with TotalScript™ RNA-Seq Kit according to the manufacter’s protocol. Libraries were sequenced on an Illumina Hiseq 1000 instrument, using a TruSeq SR Cluster Kit v3-cBot-HS (Illumina) and TruSeq SBS Kit v3-HS - 50 cycles (Illumina), using a Single Read 50 bp protocol. Libraries have been pooled in equimolar proportions and diluted to a final concentration of 12pM, according to Illumina recommandations.
GEO Accession
GSM2128414