Sequence Read Archive of Rubrobacter sp. SCSIO52915
After the DNA sample was delivered, we did a sample quality test first. Then we used this qualified DNA sample to construct BS libraryPurified DNA sample, such as genomic DNA, Bacterial Artificial Chromosome or long-length PCR productions, is sheared into smaller fragments with a desired size by Covaris S/E210 or Bioruptor firstly. Then the overhangs resulting from fragmentation are converted into blunt ends by using T4 DNA polymerase, Klenow Fragment and T4 Polynucleotide Kinase. After adding an A base to the 3' end of the blunt phosphorylated DNA fragments, adapters are ligated to the ends of the DNA fragments. The desired fragments can be purified though gel-electrophoresis, then selectively enriched and amplified by PCR. The index tag could be introduced into the adapter at the PCR stage as appropriate and we did a library quality test. At last, the qualified BS library would be used for sequencing.