Sequencing of the SSR-enrichment genomic library of Nilaparvata lugens
SSR-enrichment genomic library of  Nilaparvata lugens was created and sequenced.
The SSR-enrichment genomic libraries was prepared from Hadano-66 genomic DNA, which was extracted from approximately 50 insects, including nymphs, adults, males, and females. The SREL library was produced by the Savannah River Ecology Laboratory (University of Georgia) following the method of Glenn and Schable.26 Briefly, 2ug of BPH genomic DNA was digested with RsaI. The digested DNA was ligated with double-stranded SuperSNX linkers (5′–GTTTAAGGCCTAGCTAGCAGAATC–3′/5′–GATTCTGCTAGCTAGGC CTTAAACAAAA–3′). The linker-ligated DNA was hybridized with seven biotinylated oligo probes: (AG)12, (TG)12, (AAC)6, (AAG)8, (AAT)12, (ACT)12, and (ATC)8. The DNA fragments with a microsatellite repeat were captured using Dynabeads (Dynal, Oslo, Norway) under a magnetic field. The amount of eluted DNA was increased by using the polymerase chain reaction (PCR) to recover enriched DNA fragments using the SuperSNX primer pairs.