GSM4218147: D8 [2019A704]; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNAeasy kit (Qiagen) following manufacturer's instructions. Quality, quantitation and sizing of total RNA was evaluated using the RNA 6000 Pico assay.  (Agilent Technologies Ref. 5067-1513) on an Agilent 2100 Bioanalyzer system. Libraries were constructed using 500 ng of total RNA after Qubit quantification, the Kapa mRNA HyperPrep (Roche, #KR1352-v5.17) was used following manufacturer's instructions. Profile and quality of the libraries were assessed on a Bioanalyzer High Sensitivity DNA array (Agilent Technologies) and quantified using  QubitTM with a dsDNA High Sensibility Kit (Thermofisher)
GEO Accession
GSM4218147