GSM1260651: MN_Soctosporus; Schizosaccharomyces octosporus; MNase-Seq
Mononucleosomal DNA was isolated as described (de Castro et al. 2012). The amount of Zymolyase  20T used to prepare spheroplasts was optimized experimentally to generate a 80:20 ratio of mononucleosomes to dinucleosomes, as described (Lantermann et al. 2010). Cell suspensions of the culture of S. octosporus were treated with 50 mg of Zymolyase  20T, during 30 minutes at 30 ºC. Spheroplasts were treated with 200 units/ml of micrococcal nuclease at 37 ºC during 45 minutes.  Mononucleosomal DNA was recovered from 1.5% agarose gels and sequenced in an Illumina Genome Analyzer IIx using the single-end sequencing protocol. Mononucleosomal DNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM1260651