GSM2651154: WT_2i_ATAC_1; Mus musculus; ATAC-seq
Cells were harvested with 0.25% trypsin for 5 minutes, collected, and counted. 50,000 cells were spun down at 500xg for 5 minutes at 4°C and washed once with 50µL of cold PBS. After a second wash at 500xg for 5 minutes at 4°C, cells were lyzed in 100 ul cold lysis buffer (10 mM Tris pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630). Cells were spun for 500xg for 10 minutes at 4°C and nuclei pellets were subjected to 50 ul Transposase reaction (1X Tagmentation buffer, 2.5 ul Tn5 transposase enzyme (Illumina)) and incubated for 30min at 37°C. Subsequent steps of the protocol were performed as described in PMID: 24097267. library construction were performed as previously described  (Buenrostro et al., 2013)
GEO Accession
GSM2651154