GSM4551523: Liver_p53_homo; Mus musculus; RNA-Seq
Total RNA was extracted with QIAzol andpurified with the miRNeasyMini kit (Qiagen). RNA was quantified using the Qubit® RNA HS Assay Kit (Thermo Fischer Scientific). RNA integrity (RIN) was measured using the Bioanalyzer 2100 RNA 6000 Nano Kit (Agilent Technologies). High-quality total RNA was assessed for all samples, with RIN values ranging from 8.0 to 10.0. TruSeq® Stranded mRNA Kit (Illumina) was used to generate all libraries, according to the manufacturer's recommendations. Produced libraries were quantified by Qubit® DNA HS Assay Kit (Thermo Fischer Scientific) and Bioanalyzer High Sensitivity DNA Kit (Agilent Technologies). RNA-seq. An equimolar libraries pool (14 samples) was loaded onto Illumina NextSeq 500 platform into a High Output Kit v2.5 cartridge (2x75 bp paired-end sequencing with about 25 million clusters per sample).
GEO Accession
GSM4551523