GSM1875304: NCI-H508; Homo sapiens; RNA-Seq
Media was removed and cells were lysed on the plate by addition of 1 mL of Trizol. The Trizol solution was transferred to 1.5 mL microfuge tubes and RNA was extracted by addition of chloroform to 30% final concentration. Tubes were centrifuged at 12,000 x g for 15 minutes. The aqueous phase was removed and RNA was further purified using Maxwell® 16 Total RNA Purification Kit (Promega, Madison, WI, Cat.# AS1050) Ovation Human FFPE RNA-Seq kit (Nugen, San Carlos, CA, Cat.# 0340 and 0341)
GEO Accession
GSM1875304