Panagrellus redivivus small RNA-seq
Small RNAs were isolated from mixed cultures of P. redivivus using miRVana kit (Ambion) according to the manufacturer’s instructions. A small RNA library was then produced from the isolated RNAs using NEBNext small RNA sample prep Set 1 (New England Biolabs). The library was then size selected on a 6% PAGE gel with the cut band corresponding to ~90-120 bp. Library quality and size was confirmed prior to sequencing on a Bioanalyzer (Agilent).