GSM1692908: transgenic flies with piRNA reporter, Piwi IP [RR209]; Drosophila melanogaster; RIP-Seq
For preparation of small RNA libraries total RNA was isolated from the lysates or RNA was immunoprecipitated from the protein complexes with the indicated antibodies against PIWI protein and the RNAs were resolved by 15% or 20% urea-PAGE. Bands corresponding to piRNAs or the indicated size were excised from the gel and extracted with 400 µl of 0.3M NaCl solution at 25°C overnight. For sequencing mouse long RNAs, testicular RNAs were isolated by TRIZOL. Small RNA sequencing libraries  were prepared using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Cat No:E7300) according to manufacturer instructions.  For mouse total RNA libraries strand-specific RNA-seq libraries were prepared using TruSeq Stranded Total RNA Sample Preparation Kit with Ribo-Zero (mouse) (Ilumina). Libraries were sequenced on Illumina HiSeq platform (EMBL Heidelberg Gene core facility)
GEO Accession
GSM1692908