GSM3143183: Bone marrow Ly6G.hi neutrophils_3; Mus musculus; RNA-Seq
Neutrophils were sorted based on their expression of Dapi-CD45+Lin(CD3/B220/NK1.1)- Ly6G+Ly6C+ on Day 27 post-transplantation of tumor cells. RNAs were harvested using Trizol reagent and analyzed on an Agilent Bioanalyzer for quality assessment. cDNA libraries were prepared using 2 ng total RNA and 1 ul of a 1:50,000 dilution of ERCC RNA Spike in Controls (Ambion® Thermo Fisher Scientific) using the SMARTSeq v2 protocol. Samples were subjected to an indexed PE sequencing run of 2x151 cycles on an Illumina HiSeq 4000. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM3143183