Whole Genome Sequencing of Methyloacterium sp. strain 2A
Single colonies were selected for DNA extraction from 48-h culture on LBNS (Luria Bertani No Salts, without NaCl) medium. Genomic DNA (gDNA) was extracted manually. Library was constructed with TruSeq Nano DNA kit through TruSeq Nano DNA Sample preparation guide, part #15041110 Rev. D. Whole Genome Sequencing (WGS) of isolate 2A was carried out by an Illumina TruSeq Nano platform at Macrogen Laboratories. The number of paired-end reads was approximately 7,300,000 in pairs of 151 bp, totaling 1100 Mb of sequence data.