PA-seq of MEF_G0
G0 phase of PD6 (population doubling times) MEF cells after isolated and continously subcultured were obtained through serum starvation. Total RNA samples were isolated using TRIzol reagent (Life Technologies, NY, USA). The PA-seq libraries were constructed by following the protocol published before by Ni et al. (BMC Genomics,2013). The concentration of the RNA-seq libraries was measured by Qubit fluorometry (Life Technologies, NY, USA) before loading onto the Illumina HiSeq2000 platform.