GSM1930055: WT_mRNA_rep1; Mus musculus; RNA-Seq
Frozen tissues were homogenized in 20 mM Tris (pH=7.5), 100 mM NaCl, 10 mM MgCl2, 1% Triton X100, 0.25% sodium deoxycholate. Total RNA was isolated from Proteinase K-treated extracts using acid phenol. Following treatment with DNase I, libraries from polyA-selected RNA were generated using the TruSeq RNA Sample Preparation Kit v2 (Illumina). Total RNA was isolated from Proteinase K-treated extracts using acid phenol. Following treatment with DNase I, libraries from polyA-selected RNA were generated using the TruSeq RNA Sample Preparation Kit v2 (Illumina).
GEO Accession
GSM1930055