Illumina HiSeq 2000 paired end sequencing; Direct RNA Sequencing of polyadenylated RNA & high-depth Illumina strand-specific, short read RNA-seq of a stably RNAi-silenced nuclear 5'-3' exonuclease XRN3 mutant line in Arabidopsis thaliana
Direct RNA Sequencing of polyadenylated RNA & high-depth Illumina strand-specific, short read RNA-seq of a stably RNAi-silenced nuclear 5'-3' exonuclease XRN3 mutant line in Arabidopsis thaliana
Libraries were prepared using Illumina Tru-seq ribo-minus plant strand-specific protocol including barcoding.
Experimental Factor: xrn3-8 mutant
genotype
Experimental Factor: total RNA depleted for ribosomal RNA
fraction
Experimental Factor: Illumina strand-specific 100bp paired-end sequencing
protocol