Genome-wide DNA methylation analysis in Drosophila under dietary restriction
Wild-type Dahomey strain was reared under standard laboratory husbandry conditions, all adult female flies  fed with dietary restriction medium. They were reared at 25°C on a 12h light:12h dark cycle, at a constant humidity of 65%. At the same time, lifespan and lifetime fecundity (7d, 9d, 14d, 16d, 21d, 23d, 28d) were also measured.5ug genomic DNA was isolated by using DNAeasy Blood and Tissue Kit for library construction and bisulfite conversion and sequencing were performed at Beijing Novogene, China, using Illumina Hiseq 2500 platform, following standard protocol.