Degradation of MCHM (4-methylcyclohexanemethanol) in Sediments from Elk River, West Virginia
Sediment samples (0.24 to 0.35 g) were extracted using the PowerSoil¨ DNA isolation kit (Mo Bio Laboratories, Inc., Carlsbad, CA, USA). DNA was quantified via Qubit¨ dsDNA High Sensitivity assay (Invitrogen, Carlsbad, CA, USA). DNA samples (n = 20) were sent to the Michigan State University Genomics Core Facility for sequencing on an Illumina MiSeq (San Diego, CA, USA), where the 16S rRNA gene V4 region from both Bacteria and Archaea was amplified with barcoded and Illumina-compatible primers 515F and 806R (Caporaso et al 2011) and the amplicons normalized and pooled for 2 x 250 base pair sequencing using a standard MiSeq flow cell and a 500 cycle reagent cartridge (both v2).