GSM2994684: PAU_Nsi; Homo sapiens; RNA-Seq
RNA extraction and purification were performed 24hrs post siRNA transfection using a total RNA extraction and purification kit (Norgen, Thorold, ON, Canada) following the manufacturer's suggested instructions. Libraries for each RNA extracted were prepared using 10ug in conjunction with the Illumina paired-end stranded Total RNA-Seq ribosome depletion kit. Post preparation, libraries were paired-end sequenced (1550bp) for 40-50M reads each (Illumina HiSeq).
GEO Accession
GSM2994684