GSM1876368: MabATCC_rna_R1; Mycobacteroides abscessus ATCC 19977; RNA-Seq
Bacteria were recovered by centrifugation (10 min at 4000 x g) and re-suspended in TRI-Reagent (Sigma Aldrich). Following physical disruption using 0.1 mm zirconia-silica beads in a MagNA Lyzer (Roche Diagnostics), RNA was isolated as previously described (doi: 10.1111/mmi.12387). Briefly, RNA was isolated using miRNeasy spin columns with an additional on-column DNase treatment, according to manufacturer's instructions (Qiagen). Total RNA was treated with RiboZero (Epicentre) to deplete ribosomal RNA. Sequencing libraries were generated with the TruSeq Stranded RNA kit according to manufacturer's instructions (Illumina Inc.).
GEO Accession
GSM1876368