GSM4669688: iNSC 2, rep 3, N2B27 protocol; Mus musculus; RNA-Seq
RNA and DNA were extracted from cell pellets using the RNA/DNA/Protein Purification Plus kit (NORGEN, #47700), following the manufactured protocol. RNA samples were sequenced on HiSeq4000, at 75 bp PE after polyA selection. For the RRBS library preparation, DNA samples were digested overnight with restriction enzyme Mssp1 (Agilent), then prepared following the manufactured protocol of NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (Biolab, E7645). Finally, bisulfite conversion following Zimo kit (Qiagen, D5001) run on NextSeq 500 Mid Output Run 150 cycles.
GEO Accession
GSM4669688