Saccharum F1 Population
Total RNA was extracted Using TRIzol? (Invitrogen, USA). 5 g total RNA of each samples were used for cDNA libraries constructions. The cDNA libraries except for LA-Purple were prepared using Illumina ? TruSeq? RNA Sample Preparation Kit (RS-122-2001(4),llumina) according to the manufacturers protocol. Quality controls were performed by running 1g of library on an Agilent DNA 1000 LabChip through a Agilent Technology 2100 Bioanalyzer. A pair-end library for LA-Purple was made using Illumina ? TruSeq? RNA Sample Preparation Kit (RS-122-2001(2), Illumina)$