GSM3839829: Oxy_Mutant_polyA_RNAseq_replB; Oxytricha trifallax; RNA-Seq
Oxytricha polyA+ RNAseq protocol: Vegetative Oxytricha cells were lysed in TRIzol reagent (Thermo Fisher Scientific) for total RNA isolation according to manufacturer's instructions. Poly(A)+ RNA was then purified using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England BioLabs). The Oxytricha poly(A)+ RNA was prepared for RNA-seq using the ScriptSeq v2 RNA-Seq Library Preparation Kit (Illumina). polyA+ RNAseq (Single-read sequencing); ScriptSeq v2 RNA-Seq library preparation protocol
GEO Accession
GSM3839829