GSM1608505: GM12878_HIC_1; Homo sapiens; OTHER
25 million cells for GM12878 were cross linked and chromatin digested with HindIII. DNA overhangs were biotinylated and proximity ligated under dilute conditions to favor ligation of fragments in three-dimensional proximity. DNA was then sheared, biotinylated fragments were enriched with streptavidin beads and prepared for Illumina sequencing For ChIP-Seq, nuclear lysates were sonicated using a Branson 250 Sonifier (power setting 2, 100% duty cycle for 7 x 30-s intervals). Clarified lysates corresponding to 20 million cells were treated with 1-5ug of antibody coupled to Protein G Dynabeads (Invitrogen #10003D, New York). The protein-DNA complexes were washed with RIPA buffer and eluted in 1% SDS TE at 65°C. ChIP DNA sequencing libraries were generated according to Illumina DNA Tru-Seq DNA Sample Preparation Kit Instructions (Illumina Part # FC-121-2001, San Diego, CA).
GEO Accession
GSM1608505