Exome sequencing of B10.BR strain mice.
A genomic DNA library prep was created by sonication with a Covaris Adaptive Focused Acoustics Model S2 instrument (Covaris, Woburn, MA) followed by DNA end repair, end adenylation, end adapter ligation, and validation using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).  Target enrichment was performed using the Agilent SureSelect XT Mouse All Exon Kit, a solution-based exome capture method that uses biotinylated RNA library baits and micromagnetic streptavidin bead selection for exome enrichment, per vendor instructions.