HCC tumor: Sample 133T
Total RNA of tissues were extracted using mirVana kit (Ambion) and the quality of the RNA samples was assessed using Agilent RNA 6000 nano kit.  Total RNA was used to synthesize cDNA with random primers. Full-length cDNAs were biotinylated and captured by streptavidin-coated magnetic beads. The cDNAs were released from cap-trapped RNAs, ligated to 5' linkers including barcode sequences, and digested with EcoP15I. The detailed protocol is described in Ref [PMID:22362160]
alignment_software
BWA v0.5.9