GSM1692819: MNase-seq Standard MNase concentration rep1; Mus musculus; MNase-Seq
Nuclei were treated with MNase. The reaction was quenched by addition of EDTA to 10 mM. Proteins were removed by digestion with proteinase K, extraction with phenol and chloroform, and precipitation with ethanol. RNA was removed by digestion with RNase A, extraction with phenol and chloroform, and precipitation with ethanol. DNA was end-repaired using End-It DNA End-Repair Kit (Epicenter) and 3' ends were adenylated by treating the samples with Klenow (exo-) (NEB) in the presence of dATP.  The samples were then ligated to Illumina TruSeq adapters. The ligated products were run on agarose gel and were purified using Qiagen MinElute columns. The ligated products were then PCR-amplified using KAPA master mix. The amplified products were run on agarose gel and were purified using Qiagen MinElute columns.
GEO Accession
GSM1692819