mRNA extracted from whole plant mock treated
In vitro-grown sugarcane plantlets were maintained at 28*C with an irradiance of 60 *mol photons m-2 s-1 and 12 h photoperiod. After the development of a root system, vigorous and pathogen-free plants were divided in two halves with a scalpel. One half was used as control, immersing the root system in distilled water by 5 minute and then washed twice with distilled water. Mock plants were transferred to MS medium and kept there for seven days. After this period, whole plants were harvested and total RNA was isolated using Trizol (Invitrogen, CA, USA) as described by the manufacturer. The mRNA-seq library was constructed following the TruSeq RNA Sample Prep Kit.