RNA-Seq (Total RNA) Fetal Renal Cortex
Illumina TruSeq RNA PART# 15008136 REVISION A
EXPERIMENT_TYPE
mRNA-Seq
EXTRACTION_PROTOCOL
Qiagen TissueLyser and RNeasy Lipid Tx Mini kit
EXTRACTION_PROTOCOL_MRNA_ENRICHMENT
Part of Library Prep, Truseq RNA-Seq Sample Prep Kit
RNA_PREPARATION_INITIAL_RNA_QNTY
1ug
RNA_PREPARATION_REVERSE_TRANSCRIPTION_PRIMER_SEQUENCE
Random Primers(hexamers)
RNA_PREPARATION_REVERSE_TRANSCRIPTION_PROTOCOL
TruSeq RNA
LIBRARY_GENERATION_PCR_TEMPLATE
cDNA
LIBRARY_FRAGMENTATION
Part of Library Prep, Truseq RNA-Seq Sample Prep Kit
LIBRARY_FRAGMENT_SIZE_RANGE
120-200bp
LIBRARY_GENERATION_PCR_POLYMERASE_TYPE
Proprietary
LIBRARY_GENERATION_PCR_THERMOCYCLING_PROGRAM
98C 30 sec, 15 cycles of 98C 10 sec, 65C 30 sec, 72C 30 sec, then 72C 5 min, 4C hold
LIBRARY_GENERATION_PCR_NUMBER_CYCLES
15
LIBRARY_GENERATION_PCR_F_PRIMER_SEQUENCE
Proprietary
LIBRARY_GENERATION_PCR_R_PRIMER_SEQUENCE
Proprietary
LIBRARY_GENERATION_PCR_PRIMER_CONC
Proprietary
LIBRARY_GENERATION_PCR_PRODUCT_ISOLATION_PROTOCOL
Part of Library Prep, Truseq RNA-Seq Sample Prep Kit
GEO Accession
GSM1059515