GSM2652049: BL2Baff-1; Homo sapiens; RNA-Seq
RNA was isolated using Trizol reagent including a DNase I (Roche, Mannheim, Germany) digestion step. Library preparation for RNA-Seq was performed using the TruSeq Stranded Sample Preparation Kit (Illumina, RS-122-2201) starting from 1000 ng of total RNA. Accurate quantitation of cDNA libraries was performed using the QuantiFluor TM dsDNA System (Promega). The size range of nal cDNA libraries was determined applying the SS-NGS-Fragment 1-6000 bp Kit on the Fragment Analyzer from Advanced Analytical (320 bp). cDNA libraries were amplied and sequenced by using the cBot and the HiSeq2000 from Illumina. Non stranded mRNA-Seq
GEO Accession
GSM2652049