PacBio RNA-Seq of Ephedra sinica : XWT, young stems
PCR amplification and size fractionation (16 kb, 0.56 kb) were conducted using the KAPA HiFi PCR Kits (Kapa Biosystems, USA) and BluePippin Size Selection System (Sage Science, USA); Libraries were constructed using SMRTbell Template Prep Kit 1.0 (PacBio, USA)