RNA-Seq of CHO cell:CHO-K1 cells in stationary cultivation phase
A suspension and serum-free adapted sub clone of the CHO-K1 parental cell line (ATCC CCL-61) provided by Novo Nordisk A/S (Denmark) grown in HyClone CDM4CHO with L-Glutamine medium, 0.5% P/S, and 0.4% Anti-Clumping Agent. Total RNA was extracted from 2 mio cells using chloroform and RNeasy mini kit (Qiagen, USA) following manufacturer�s instructions. cDNA library and next-generation sequencing were carried out by AROS Applied Biotechnology (Aarhus, Denmark) in a Illimina Hiseq 2000 system for paired-end sequencing.