GSM3142973: PerPool_3; Homo sapiens; RNA-Seq
RNA was isolated from fresh brushings,  utilizing Ambion's ToTALLY RNA™ RNA Isolation Kit (Part Number AM1910, ©Ambion, Inc.).   RNA quality was assessed by utilizing an Agilent Bioanalyzer and assigning an RNA Integrity Number (RIN) to each sample. RNA sequencing libraries were prepared using the Illumina TruSeq Stranded Total RNA Sample Preparation Kit.  Briefly, 500ng of total RNA was purified by Ribo-Zero to remove rRNA and fragmented by divalent cations under elevated temperature. The fragmented RNA underwent first strand synthesis using reverse transcriptase and random primers.  Second strand synthesis created the cDNA fragments using DNA polymerase I and RNaseH.   The cDNA fragments then went through end repair, adenylation of the 3' ends, and ligation of adapters.  The cDNA library was enriched using 10 cycles of PCR and purified.
GEO Accession
GSM3142973