GSM4551648: 2D_CM_CHIR_2; Homo sapiens; RNA-Seq
RNA was extracted using trizol and precipitated using glycogen according to manufacturers instructions. Clean-up and DNAse protocols was performed using Rneasy MinElute Cleanup Kit (QIAGEN) libraries were constructed with Nugen Ovation RNA-Seq system V2 (for SPIA amplifications and cDNA generation) coupled with the Ovation Ultralow System (NuGEN). Libraries were read with 50bp SR Rapid Mode on a HiSeq1500. Barcode information : SampleULN_i7index _LaneNumber_ReadNumber.fastq
GEO Accession
GSM4551648