Illumina NovaSeq 6000 paired end sequencing; ATAC-seq of primary mesenchymal stem cell (MSC)-derived osteoblasts (differentiated with BMP2) from 4 human donors
ATAC-seq of primary mesenchymal stem cell (MSC)-derived osteoblasts (differentiated with BMP2) from 4 human donors
Primary bone-marrow derived human MSCs isolated from 4 healthy donors were plated in osteogenic media at 15,000 cells/cm2 and stimulated the next day with recombinant human BMP2 in serum-free osteogenic media. Cells were harvested at 72 hours following BMP2 treatment. The ATAC-seq libraries were generated following a previously published protocol (Buenrostro et al., Nature Methods, 2013). The nuclei were isolated from the 100,000 cells. The isolated nuclei were treated with Tn5 Transposes from Nextera DNA Sample Preparation Kit and the transposed DNA was purified using a Qiagen MinElute Kit. The transposed DNA libraries were amplified by PCR for 12 cycles and isolated by Ampure XP beads.
Experimental Factor: individual
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