GSM4552064: BJ NT r2 Late S; Homo sapiens; OTHER
Genome-wide replication timing profiles were constructed as previously described (Marchal et al., 2018). Briefly, cells were pulse labeled with BrdU and separated into early and late S-phase fractions by flow cytometry.  From the total gemnomic DNA extracted from each fraction, BrdU-labeled DNA was enriched using anti-BrdU immunoprecipitation. Sequencing libraries of BrdU-labeled DNA from early and late fractions were prepared by NEBNext Ultra DNA Library Prep Kit for Illumina (E7370).
GEO Accession
GSM4552064