GSM1533373: Extracted RNA from Control small intestine organoids Control_SI_4; Mus musculus; RNA-Seq
Organoids were freed from Matrigel and resuspended in RTL buffer (Qiagen). RNA was extracted from Cdx2null SI, Control SI, Control Sto and Cdx2+ Sto organoids using the RNeasy Mini RNA Extraction Kit (Qiagen). 10ng of total RNA per sample was processed using the previously described CEL-seq technique. Libraries were sequenced on an Illumina NextSeq500 using 75bp paired end sequencing.
GEO Accession
GSM1533373