Encore Complete DR RNA-seq of Lonchura striata domestica: juvenile female forebrain
Tissues were dissected out then minced and homogenized on ice. RNA was extracted using standard TRIzol extraction. 2 µg total RNA was DNase-treated using 2U rDNase I (Ambion) at 37°C for 25 minutes. DNase-treated total RNA was purified using RNA Clean and Concentrator 25 (Zymo) then 120 ng of this sample was prepared for sequencing using the Encore Complete DR RNA-seq Library System (NuGEN) according to the manufacturer's protocol.