Identification of immune responsive genes in Golden mahseer (Tor putitora)
One experiment for enrichment of immune responsive genes by challenging the fishes with Aeromonas hydrophilla was performed. The LD50 was determined and a standardized dose was injected to test samples while control samples were injected with PBS solution. The specimens were monitored up to 72 hr and samples from both the groups were collected at different time intervals (0 hr, 3 hr, 6 hr, 12 hr, 48 hr and 72 hr). The liver tissue collected at 0 hr (control only), 6 hr and 12 hr were processed  for RNA isolation followed by library preparation (TruSeq RNA Library Preparation Kit v2) and sequencing on Illumina Miseq. One library was prepared using pooled samples from control while another two were prepared using individual test samples.