RNA-seq of Malus domestica:  Hand-pollinated ovule 18 days Rep 2
RNA was isolated using Norgen Plant/Fungi Total RNA Purification Kit (Norgen Biotek Corp., ON). DNA was removed from RNA using TURBO DNA-free kit (Thermo Fisher Scientific, MA). DNased RNA was sent to GENEWIZ (GENEWIZ Inc., NJ) where forty-eight RNA libraries were constructed using NEB Next Ultra RNA Library Prep Kit for Illumina with polyA selection (New England Biolabs Inc., MA) with total RNA and were sequenced with an Illumina Hiseq 2000 (Illumina Inc., CA).