Partially fed adult female salivary glands 454 library
The cDNA library was normalized using Trimmer cDNA normalization kit (Evrogen, Moscow, Russia. Normalized DNA was fragmented by sonication, and ends were enzymatically blunted and ligated to customized 454 adaptors. Emulsion PCR reactions were performed according to the manufacturer (Roche 454 Life Sciences). Sequencing adapters (A and B) were automatically removed from the reads using signal processing software (Roche 454 Life Sciences).