GSM4419012: Mixed, barRNA-seq barcodelets; Mus musculus; RNA-Seq
For each dataset, cells were mixed at equal concentrations and resulting pool was prepared for 10X Chromium scRNA-seq XXh later. Cells were prepared by trypsinization, resuspension in PBS + 0.04% BSA, and repeated filtering through a 70 µM cell strainer. Cells were then counted and loaded onto the 10X Genomics Chromium Single Cell Gene Expression chip using the manufacturer's protocol, aiming for 10,000 cells. Library prep was performed according to manufacturer's protocol with modified SPRI purification to prepare transcriptome (long cDNA > 500bp) and barcodelet (short cDNA <500bp) libraries. The transcriptome is pooled with the barcodelet at a molar ratio of 9:1 for Illumina Nextseq sequencing.
GEO Accession
GSM4419012