GSM3081818: csr-1 input znfx-1(ne4354) 2; Caenorhabditis elegans; ncRNA-Seq
Worms or immunoprecipitates from worm lysates were treated with trizol. For input libraries, the extracted RNA was subjected to mirVana enrichment for small RNAs, before library construction. Libraries were constructed by dephosphorylating g and b phosphates of 22G-RNAs using homemade 5' polyphosphatase PIR-1, ligating 3' adaptor linker using T4 RNA ligase 2 (NEB), ligating 5' adaptor linker using T4 RNA ligase 1(NEB), converting RNA to cDNA using Superscript III (Thermo Fischer Scientific), and then amplified by PCR.
GEO Accession
GSM3081818