GSM3602427: M2G_3_0D; Caulobacter vibrioides; OTHER
Cells were lysed by mixer mill using 6 cycles of 3 min at 15Hz. In between cycles, the chamber was resuspended in liquid nitrogen to keep frozen. Ribosome protected mRNA fragments were size selected via gel purification. mRNA fragments were ligated to 5' adenylated DNA oligo. After reverse transcription, the single stranded DNA was circularized, rRNA fragments removed using biotinylated DNA probes, and PCR amplified [Oh et al,. Cell 147, 1295 (2011)].
GEO Accession
GSM3602427