Illumina sequencing of Fom HCT strain: Fom001_HCT#3
We sequenced six strains that were resulted from horizontal chromosome transfer (HCT) experiments (DOI: 10.1111/mpp.12927). Three HCT strains, including Fom001_HCT#3, Fom001_HCT#4 and Fom001_HCT#5, were obtained by co-incubating the donor strain Fom001SIX6#11 and a recipient strain Fo47. Fom005_HCT#2 was obtained by co-incubating the donor strain Fom00SIX6#1 with a recipient strain Fo47, while Fom005_HCT#3 and Fom005_HCT#4 were obtained by co-incubating Fom005SIX6#87 with a recipient strain Fo47. The three donor strains carry a hygromycin (HYG) resistance gene, inserted inside of the SIX6 ORF, while the recipient strain Fo47 carrys a phleomycin resistance gene (BLE, confers resistance against zeocin), inserted randomly into the genome. HCT was assessed using PCR and CHEF-gel analysis. We sequenced these strains for confirming HCT and determining sequences that were transferred.