GSM2127561: IL-1R8-/-_NT_1_L1; Mus musculus; RNA-Seq
Total cortical RNA was extracted with TRI reagent (Sigma) and subsequently treated with DNaseI (Qiagen) and precipitated using potassium acetate. To prepare each sample equal amounts of purified total RNA from 3 mice were pooled together. 3 samples (biological replicates) per genotype were sequenced in HiSeq 2500 apparatus (Illumina). Service provided by Fasteris S.A. Paired-End stranded RNA libraries were prepared for sequencing using standard Illumina protocols (TruSeq Stranded mRNA Sample Prep Kit). Service provided by Fasteris S.A.
GEO Accession
GSM2127561