DNA-Seq of human: adult female blood
Genomic DNAs from 3 monozygotic twin pairs were isolated from the peripheral blood and were used to construct a genomic DNA library for WGS. DNA was extracted and sheared into fragments, which were then puried by gel electrophoresis. DNA fragments were ligated with adaptor oligonucleotides to form paired-end DNA libraries with an insert size of 500 bps. To enrich libraries for sequences with 5' and 3' adaptors, we used ligation-mediated PCR amplication with primers complimentary to the adaptor sequences. The DNA libraries were sequenced to generate 90 bps pair-end reads with at least 30x average genome coverage per sample.