Wolbachia free Male
500ng of BstZ17I-digested total genomic DNA was utilized per sample for each library. All PCRs were performed with PlatinumŽ Taq DNA Polymerase High Fidelity polymerase from Life technologies (Cat# 10790-020) using appropriate primers (Table S1). 16S rRNA amplicons from PCRs were separated by agarose gel electrophoresis and subsequently extracted using the Qiagen QIAquick Gel Extraction Kit (Cat# 28706). Kappa Biosystems DNA standards (Cat# KK4903) were used for calibration of DNA concentration used for sequencing by qPCR with Illumina adaptors (Table S1). qPCRs were performed with SYBRŽ GreenER qPCR SuperMix Universal from Life Technologies (Cat# 11790-01k). Sequencing was performed on Illuminas MiSeq platform using 250bp paired end reads