GSM2995515: WT_18C_NETSEQ; Schizosaccharomyces pombe; RNA-Seq
RNAPII was immunopurified using anti-FLAG M2 affinity gel (Sigma) and nascent RNA was isolated using Qiagen miRNeasy Mini Kit. Custom oligos were used in library construction for sequencing on the Illumina platform. Libraries were analyzed using the Agilent D1000 TapeStation (Agilent) and sequenced on the Illumina MiSeq platform.
GEO Accession
GSM2995515