Mesenchymal Rep 1 HiSeq
MCF10AT cells were maintained in DMEM/F12 supplemented with 5% donor horse serum, 20 ng/ml epidermal growth factor, 10 g/ml insulin, 1 ng/ml cholera toxin, 100 g/ml hydrocortisone, 50_U/ml penicillin, and 50 g/ml streptomycin.Cells were crosslinked with 1% formadehyde at room temp. for 10 minutes.  Nuclei were then purified and digested with MNase (15 units/106 nuclei) at 37 degrees for 30 minutes.To induce EMT, MCF10AT cells were culture in the presence of recombinant human TGFB1 (2.5 ng/ml) for 12 days.