Transcriptomic change in response to high ambient temperature in two Arabidopsis thaliana ecotypes
Project goal: to observe transcriptomic change in response to high ambient temperature in two Arabidopsis thaliana ecotypes: Col-0 and Sij-4.   Library preparation: one hundred 6-day-old seedlings of Col-0 or Sij-4 grown at 23 ºC or 27 ºC in growth chambers (GR-36, Percival Scientific, Canada) in SD were harvested around 1.00 PM (5 hours after the beginning of the light regime). The RNA was extracted using Isolate II RNA plant kit (Bioline Pty Ltd, Australia) and RNASeq was done with Illumina HiSeq2000 platform by BGI (BGI, China). Three and two biological replicates were used for Col-0 and the Sij-4 samples respectively