Cassida rubiginosa foregut expression
Three C. rubiginosa adults were dissected on an RNaseZap-treated (Sigma-Aldrich, St. Louis, MO, USA) plate and both pairs of foregut symbiotic organs were then freed from the rest of the insect?s body. The symbiotic organs, as well as their respective symbiotic organ-free whole beetles were immediately subject to RNA extraction using the Qiagen RNA Extraction kit (Hilden, Gemany) according to the manufacturer?s instructions. Sequencing of the resulting six samples (3X symbiotic organs; 3X whole insects) was performed at the Max Planck Sequencing Core on the HiSeq 2500 Sequencing System from Illumina (http://www.illumina.com/webcite), utilizing the paired-end 100 bp technology at a depth of 100 million reads.