16S rRNA profiling of mouse: female ileum content
Genomic DNA was purified using the PowerSoil DNA Isolation Kit (MO BIO Laboratories, Carlsbad, CA) following the manufacturers recommendations. A dual-index amplicon sequencing method was employed for PCR amplification of the V4 region of the 16S rRNA gene, and sequencing was carried out on an Illumina MiSeq (San Diego, CA) to generate 250 base pair paired-end reads.