RNA-Seq of CHO cell: DG44 IgG producing cells treated with NaBu 48h after seeding
A recombinant suspension CHO DG44 cell line stabile expressing a human IgG (DG44IgG), kindly provided by Symphogen A/S (Denmark) in PowerCHO medium with 5 mM L-Glutamine, 0.1 mM MEM NEAA, and 0.4% Anti-Clumping Agent. Total RNA was extracted from 2 mio cells using chloroform and RNeasy mini kit (Qiagen, USA) following manufacturer�s instructions. cDNA library and next-generation sequencing were carried out by AROS Applied Biotechnology (Aarhus, Denmark) in a Illimina Hiseq 2000 system for paired-end sequencing.