GSM4419107: S1_d4_dmso; Mus musculus; RNA-Seq
Single cells were obtained from organoids by mechanical disruption and subsequent digestion with TRYPLE Express. Cells were used for FACS sorting to discard debris and dead cells. Obtained cellular suspensions were loaded on a 10x Genomics Chromium Single Cell instrument to generate single cell GEMs Single cell RNA-Seq libraries were prepared using GemCode Single Cell 3' Gel Bead and Library Kit according to CG00052_SingleCell3'ReagentKitv2UserGuide_RevB.
GEO Accession
GSM4419107