GSM2474509: E861A Ifih1 knockout Heart replicate 1; Mus musculus; RNA-Seq
Following tissue homogenization with a mechanical blender, RNA was isolated from TRIsure reagent (Bioline) using Direct-Zol columns (Zymo Research) as described by the manufacturer. cDNA was synthesized using iScript Advanced Kit (BioRad) using 1.2-7.5μg RNA per tissue. 200ng of cDNA was used for mmPCR-seq (www.ncbi.nlm.nih.gov/pubmed/24270603). Loci containing known A-to-I editing sites were PCR amplified using multiplexed primer pools. PCR amplicons were used for high throughput sequencing and A-to-I editing frequencies were determined as described by Zhang and colleagues (www.ncbi.nlm.nih.gov/pubmed/24270603).
GEO Accession
GSM2474509