Illumina HiSeq 2500 paired end sequencing; Expression profiling experiment on Pkd1-mutant mice and mice induced with nephrotoxin DCVC
Expression profiling experiment on Pkd1-mutant mice and mice induced with nephrotoxin DCVC
Renal injury was induced one week after tamoxifen administration by a single i.p. injection of S-(1,2-dichlorovinyl)-L-cysteine (DCVC) (15mg/kg).  Mouse kidneys were snap frozen in N2, stored in -80 mRNA was isolated from total RNA using the oligo-dT magnetic beads. After fragmentation of the mRNA, a cDNA synthesis was performed. This was used for ligation with the sequencing adapters and PCR amplification of the resulting product. The quality and yield after sample preparation was measured with a DNA 1000 Lab-on-a-Chip. The expected broad peak between 300-500 bp was observed.  The Illumina® mRNA-Seq Sample Prep Kit was used to process the sample(s). The sample preparation was performed according the Illumina protocol \"Preparing Samples for Sequencing of mRNA\" (1004898 Rev. D). Briefly, mRNA was isolated from total RNA using the oligo-dT magnetic beads. After fragmentation of the mRNA, a cDNA synthesis was performed. This was used for ligation with the sequencing adapters and PCR amplicification of the resulting product. The quality and yield after sample preparation was measured with a DNA 1000 Lab-on-a-Chip (see appendices). The size of the resulting products was consistent with the expected size distribution (a broad peak between 300-500 bp on a DNA 1000 chip).
Experimental Factor: renal injury
disease
Experimental Factor: DCVC
compound
Experimental Factor: 2
time