"ATAC-seq of 50,000 HUVECs treated with Ethanol": Sample FUNGEI-ATAC-N726-S505
"We followed the protocol by Buenrostro et al. (2013) to lyse 50,000 cells and prepare ATAC-seq libraries, with the exception that we used the Illumina Nextera Index Kit (Cat #15055290) in the PCR enrichment step. Individual library fragment distributions were assessed on the Agilent Bioanalyzer and pooling proportions were determined using the qPCR Kapa library quantification kit (KAPA Biosystems). Library pools were run on the Illumina NextSeq 500 Desktop sequencer in the Luca/Pique-Regi laboratory. Barcoded libraries from three ATAC-seq samples, performed with 25,000, 50,000 and 75,000 cells, were pooled and sequenced on multiple sequencing runs for 100M 38bp PE reads."