UluPandan February time point 11 gDNA 454 runs
DNA libraries for pyrosequencing were prepared from extracted DNA  from the October and February samples, which were nebulized to a size range of 500-1000bp. Each library was prepared from 500 ng of DNA. Library preparation was performed using the GS FLX Titanium Rapid Library Preparation Kit (Roche Applied Science), following the manufacturer’s protocol.