GSM4419119: R213G_Bleo_4; Mus musculus; miRNA-Seq
Seven days after treatment, mice were euthanized by isoflurane and thoracotomy. Lungs were flushed with 10 mL PBS in the right ventricle, frozen in liquid nitrogen, and stored at -80˚ C. Frozen lungs were pulverized by mortar and pestle. Pulverized lungs were lysed in 2 mL of Qiazol (Qiagen) and homogenized using a Tissue Tearor (Biospec Products). RNA was isolated using the miRNeasy Mini Kit with DNA digestion using the RNA-free DNase kit (Qiagen). RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM4419119