GSM2652096: mouse014_d7_M; Mus musculus; RNA-Seq
Total RNA was immediately extracted from the head of the pancreas using TRIzol (Ambion). RNA was treated with DNase I (Zymo Research) at room temperature for 20 minutes. The concentration of DNase I-treated RNA was measured using a Qubit 3.0 Fluorometer (Thermo Fisher Scientific) and the integrity of the DNase I-treated RNA was analyzed on the Agilent TapeStation. All DNase I-treated RNA samples used for RNA library construction had RNA Integrity Numbers (RINs) above 6.4. RNA libraries were prepared for sequencing using Illumina TruSeq Total Stranded RNA sample preparation kit with ribosomal depletion. Adaptor-ligated fragments were amplified by PCR for nine cycles. The quality and size of the final library preparations were analyzed on an Agilent TapeStation. RNA-Seq using Illumina NextSeq 500 NGS platform, with approximately 40 million paired-end 75 bp reads for each sample.
GEO Accession
GSM2652096