RNA-Seq of Pregnancy 1 Day 6 BalbC Luminal Mammary Epithelial Cells
Total MECs or FACS-sorted MECs were collected and homogenized in TRIzol (Thermo Fisher Scientific, #15596018) for RNA extraction. Double stranded cDNA synthesis and Illumina libraries were prepared utilizing the Ovation RNA-seq system (V2) (Nugen Technologies, #7102-32). RNA-seq libraries were prepared utilizing the Ovation ultralow DR multiplex system (Nugen Technologies, #0331-32). Each library (n=2 per experimental condition) was barcoded with Illumina True-seq adaptors to allow sample multiplexing, followed by sequencing on an Illumina NextSeq500, 76bp single-end run.