GSM2055543: 2 WAP; Brassica napus; RNA-Seq
The total RNA from the four samples were extracted and purified using the RNAprep pure Plant Kit (DP 432, TIANGEN BIOTECH (BEIJING) CO., LTD) according to the manufacturer’s protocol. RNA purity was checked with NanoPhotometer® spectrophotometer (IMPLEN, CA, USA). The concentration was measured with Qubit® RNA Assay Kit and Qubit® 2.0 Fluorometer (Life Technologies, CA, USA). The integrity was assessed using the Agilent 2100 Bioanalyzer and RNA Nano 6000 kit (Agilent Technologies, CA, USA). cDNA libraries for samples from the four time points of seed development were constructed using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) according to the manufacturer’s protocol. Library quality was assessed using the Agilent 2100 Bioanalyzer. The library preparations were sequenced and 100-bp paired-end reads were generated by an Illumina Hiseq 2000 platform.
GEO Accession
GSM2055543