Input Control ChIP-Seq of PrePregnancy CAGMYC Basal Mammary Epithelial Cells on Dox Day 5
FACS-sorted MECs were used for chromatin pulldowns using antibody specific for H3K27ac histone marks (Abcam, #ab4729). ChIP samples were amplified and barcoded using Clontech DNA Smart ChIP-Seq kit (Clontech, #634866) in accordance with the manufacturers instructions, then pooled for sequencing on an Illumina NextSeq500, 76bp single-end run.