GSM1608299: MCF7 E2 rep1; Homo sapiens; ChIP-Seq
Cells were crosslinked with 1% Formaldehyde for 10  minutes and quenched with 1.25 M Glycine. The nuclear pellets were sonicated to the fragment range of 200-400 bp. Sonicated extracts were incubated with specific antibodies at 4˚C overnight and ChIP-immune complexes were pulled down using Protein A sepharose. Library preparations were done using NEBnext Ultra DNA library preparation kit according to the manufacturer’s instructions.
GEO Accession
GSM1608299