Reversal of selective pressures acting on the immune response during human evolution
The standard NimbleGen array capture protocol (Albert, Molla et al. 2007) was modified to allow sequencing on the Illumina Genome Analyzer II (Almomani, van der Heijden et al. 2011). For each sample, an Illumina paired end (PE) library was created following the manufacturer’s instructions with minor modifications. Sequence capture was done according to Roche NimbleGen’s instructions. Each sequencing library was run on a lane of an Illumina Genome Analyzer II, which generated an average of 70M 76 bp PE reads per lane.