GSM4550128: Input_18h; Mus musculus; ChIP-Seq
Lysates were prepared from sonicated nuclei and histone-DNA complexes were isolated with indicated antibodies. ChIP-seq libraries were prepared using Illumina's TruSeq ChIP sample preparation kit (Illumina, catalog no. IP-202-1012) according to manufacturer's specifications, with the addition of size selection (left side at 0.9x, right side at 0.6x) using SPRIselect beads (Beckman Coulter, catalog no. B23318). Library size was determined (average 351 bp, range 333-372 bp) using the Agilent Bioanalyzer 2100, followed by quantitation using real-time PCR using the KAPA Library Quant Kit for Illumina (KAPA Biosystems catalog no. KK4835). Libraries were then pooled and sequenced (1x75bp) on the Illumina NextSeq 500 platform according to manufacturer's instructions. Bclfastq2 v 2.15.04 (default parameters) was used to convert reads to fastq.
GEO Accession
GSM4550128