GSM3080964: KOD3_6; Mus musculus; RNA-Seq
Total RNA was harvested using RNeasy (Qiagen, Valencia, CA) and measured for quality using the Agilent 2100 Bioanalyzer, and Qubit 2.0 Fluorometer. All RNA integrity numbers (RIN) ranged between 8.2-9.7. The total RNA samples were submitted to the Beijing Genomics Institute (BGI) for transcriptome sequencing. In brief, magnetic beads with Oligo (dT) were used to isolate mRNA. The mRNA was fragmented and then constructed into HiSeq 2000 strand-specific libraries. The 2 × 100-nt paired-end reads were generated by Illumina HiSeqTM 2000. Clean reads filtered from raw sequence reads were returned from BGI.
GEO Accession
GSM3080964