GSM2474586: old ISCs, rep3; Drosophila melanogaster; RNA-Seq
Young and old midguts were dissociated and ISCs were isolated by FACS as described in Tauc et al. (2014). The ISCs were collected into RNAlater solution (Ambion), snap-frozen in liquid nitrogen and sent to MFT Services (Tübingen) for RNA isolation and sequencing. Sequencing libraries were prepared with the TruSeq RNA Sample Preparation Kit v2 (Illumina, San Diego, CA) according to the manufacturer's instruction. RNA libraries were prepared for sequencing using standard Illumina protocols.
GEO Accession
GSM2474586