GSM3478735: Hop1 ChIP for ndt80Δ T=6 spike-in rep1; Saccharomyces cerevisiae; ChIP-Seq
25 ml of the meiotic cells were collected at different time points and fixed for 30 min in 1% formaldehyde. The formaldehyde was quenched with 125 mM glycine. Samples were processed as described in (Blitzblau et al., 2012). For quantitative ChIP-Seq (SNP-ChIP), S288c crosslinked meiotic cells were added to respective samples at 10-20% prior to ChIP processing (Vale-Silva et al., 2018). Libraries for ChIP sequencing were prepared by PCR amplification with TruSeq adaptors (Illumina) as described in (Sun et al., 2015). Quality of the libraries was checked on 2100 Bioanalyzer or 2200 Tapestation. Libraries were quantified using qPCR prior to pooling. The ChIP libraries were sequenced on Illumina HiSeq 2500 or NextSeq 500 instruments at NYU Biology Genomics core to yield 50 or 51 bp single-end reads or 100 bp single-end reads for quantitative ChIP-seq.
GEO Accession
GSM3478735