Small RNA of 24h after inoculation
Small RNAs of 18~30nt were purified from the total RNA by 15% polyacrylamide gel electrophoresis (PAGE). A pair of Solexa adaptors was ligated to the 5′ and 3′ termini of the small RNAs, then reverse transcription was performed by Super-Script II Reverse Transcriptase (Invitrogen) and amplified using 15 PCR cycles to produce sequencing library. The final quality of the cDNA libraries were examined with the Agilent 2100 Bioanalyzer. The small RNA fragments were finally sequenced by Hiseq2, 500 platforms.