GSM1685425: Control Sample_frontalcortex_HITS_CLIP_3; Homo sapiens; RIP-Seq
For HITS-CLIP, autopsy-derived frozen brain tissues from control, disease control (ALS), DM1 and DM2 were pulverized in liquid nitrogen, UV crosslinked and fragmented using RNase A. Lysates were denatured, incubated on ice for 10 min, followed by addition of anti-MBNL2 and immunoprecipitation at 4°C for 2 hr.  MBNL2-RNA complexes were size selected, proteinase K treated, and the RNA was purified using RNA phenol chloroform extraction.
GEO Accession
GSM1685425