degradome-seq of Dendrobium huoshanense: three-year old stems
The Dendrobium stems were collected from three species [Dendrobium officinale (dof), Dendrobium huoshanense (dhu), and Dendrobium williamsonii (dwi)]. Total RNA was extracted by using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer's. Poly(A) RNA was purified by using poly-T oligo-attached magnetic beads after two rounds of purification. Because of the 3 cleavage product of the mRNA contains a 5-monophosphate groupthe 5' adapters could be ligated to the 5' end of the 3 cleavage product by RNA ligase. After cDNA synthesis and PCR amplification, 50-bp single-end sequencing was performed on an Illumina Hiseq 2500 (LC Bio, China) following the vendor's recommended protocol.