GSM4668599: L_25; Bos taurus; RNA-Seq
RNA was extracted with the RNeasy Plus Universal Mini Kit (Qiagen, Hilden, Germany) and quantified using a NanoDrop for 260/280 (Min = 1.93, Max = 2.10) and 260/230 (Min = 1.53, Max = 2.23) ratios as well as Quant-iT RiboGreen Assay Kit for RNA concentration (Min = 280.3 ng/µL, Max = 2680 ng/µL; Invitrogen, Carlsbas, CA). RNA integrity was measured with the Agilent TapeStation/BioAnalyzer (Agilent Technologies, Santa Clara, CA) for RNA quality (Min = 5.7, Max = 9.5). RNA-seq library creations were strand specific. RNA sequencing analysis was conducted on the Illumina HiSeq 2500 platform and multiplexed with 21 samples per lane (42 samples total: 14 liver, 14 muscle, and 14 cerebrum) using 50-bp paired-end reads at a depth of 2 × 10.4M reads/sample in both forward and reverse directions.
GEO Accession
GSM4668599