EPRS shRNA 84 Replicate 3
Cells expressing EPRS shRNA vectors were generated.  Seventy-two hours after infection, cells were lysed and total RNA was purified using a Qiagen RNeasy kit according to the manufacturer’s protocol. RNA was stored at -80°C until submission to the Mount Sinai Genomics Core Facility for ribosomal RNA depletion, cDNA library preparation, and sequencing using paired-end, 100nt reads on an Illumina HiSeq 2000.