RNA-Seq of mus musculus: adult male IRE-treated tumor
Total RNA was isolated and assessed for quality using an Agilent Tapestation 4200, and samples with an RNA integrity number of 7 or greater were used to generate cDNA libraries using Illuminas TruSeq Stranded mRNA Sample Prep Kit starting with 1 ug of RNA and sequenced Single Read 75 basepairs (SR75) on an Illumina HiSeq4000 sequencer.