GSM4550176: s_veh3; Mus musculus; RNA-Seq
Mouse leptomeninges were microdissected, dissociated and FACS sorted. RNA was extracted from lysates using RNeasy Mini columns (Qiagen). After RiboGreen quantification and quality control by Agilent BioAnalyzer, 2 ng of total RNA (RIN 9.7 - 10)  underwent amplification using the SMART-Seq v4 Ultra Low Input RNA Kit (Clonetech catalog # 63488), with 12 cycles of amplification. Subsequently, 10ng of amplified cDNA was used to prepare libraries with the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) using 8 cycles of PCR. Samples were barcoded and run on a HiSeq 4000 in a 50bp/50bp paired end run, using the HiSeq 3000/4000 SBS Kit (Illumina).
GEO Accession
GSM4550176