Illumina HiSeq 4000 sequencing; RNA sequencing in islets from Rip-Cre CDK8fl/fl and CDK8fl/fl mice with or without metabolic stress.
RNA sequencing in islets from Rip-Cre CDK8fl/fl and CDK8fl/fl mice with or without metabolic stress.
RNA was extracted using PicoPure columns with on-column DNase treatment. Quality was verified on Bioanalyzer Islets were isolated from mice (with or without STZ) and picked manually before being frozen down as a pellet TruSeq Stranded mRNA Library Prep Kit
Experimental Factor: age
6
Experimental Factor: genotype
CDK8fl/fl
Experimental Factor: stimulus
none