GSM2652191: mNPC_shNup153_3_2; Mus musculus; RNA-Seq
Three days after electroporation of plasmids encoding shRNA-EGFP using mouse NeuPCs, GFP-positive cells were sorted directly into Trizol-LS (Invitrogen). RNA was isolated according to the manufacturer’s instructions and digested with TURBO DNase (Life Technologies). RNA integrity numbers (RIN) were assessed using the Agilent Tape Station before library preparation. Poly(A)+ RNA was selected and fragmented to prepare sequencing libraries using the Illumina TruSeq Stranded RNA sample preparation kit. Libraries were sequenced single-end 50 bp (SE50) using an Illumina HiSeq 2500 sequencer at the Next Generation Sequencing (NGS) core at the Salk Institute according to manufacturer’s instructions (Illumina).
GEO Accession
GSM2652191