ITS-Seq: 4 representative Chinese arable soils: Sample Dr2.ITS
Soil DNA was extracted with FastDNA Spin kit for Soil DNA extraction kit. The V3–V4 region of the 16S rRNA gene was amplified in triplicate using the barcoded 515fF and 806R primer set. PCR products were quantified, purified and sequenced by Illumina Miseq platform for assessment of bacterial diversity and community structure. We design the experiment to explore the association of rhizopheric microbiome with soil biological fertility. So we sterilized 4 representative Chinese arable soils (indicated as A, B, D anr R soil) and randomly inoculated with the microbiomes (a, b, d and r microbiome) from the four soils. After crop harvest, we sequenced the 16s and ITS amplicon from the 64 soil-microbiome combinations. 
alignment_software
UCLUSTER