GSM3476463: spt6-S8E RNA Rep2; Saccharomyces cerevisiae; RNA-Seq
Yeast cultures of the indicated genotypes were grown at 30C and in YPD conditions and used for RNA extraction using acid phenole method  as described in materials and methods. ChIPs were performed as described in materials and methods with anti-H3 antibody. Isolated ChiP DAN was quantified  and used for the generation of libraries using Kappa Hyper kit. For RNA seq: RNA was extracted using acid-phenol method (Collart and Oliviero, 2001) and was quantified spectrophotometrically. 2.5g of total RNA was used to deplete rRNA using the Ribo-zero kit (Illumina). ERCC spike-in controls were added to the RNA samples after rRNA clean-up and before proceeding on to the library preparation. Stranded RNA-seq libraries were prepared using TruSeq Stranded Total RNA sample preparation according to manufacturer's instructions.  The libraries were sequenced on Illumina HiSeq 2500, paired-end 50bp reads).  For ChiP-seq ChIP was performed as described in the text and libraraies were prepared using akppa hyprep kit using manufacturers instructions.
GEO Accession
GSM3476463