GSM3602131: Chae rep2; Homo sapiens; RNA-Seq
High quality total RNA was extracted from samples using Nucleospin RNA kit of Macherey-Nagel and eluted in RNAse free water. Library preparation was performed by the Functional Genomics Laboratory (FGL),  a QB3-Berkeley Core Research Facility at UC Berkeley. mRNA enrichment was performed on total RNA using polyA selection with the Invitrogen Dynabeads mRNA Direct kit. Subsequent library preparation steps of enzymatic fragmentation, adapter ligation and cDNA synthesis were done on the enriched RNA on an Apollo 324™ liquid handling system, with PrepX™ RNAseq Library Prep Kits (WaferGen/now TakaraBio). 15 cycles of PCR amplification was used for index addition and library fragment enrichment. Libraries were sequenced on an Illumina HiSeq4000 by the Vincent J. Coates Genomics Sequencing Laboratory at UC Berkeley, supported by NIH S10 OD018174 Instrumentation Grant.
GEO Accession
GSM3602131