Whole Genome Sequencing, Cocodrie
Genomic DNA was extracted from leaf tissue using Qiagen DNeasy kit. Integrity of DNA was assessed by Bioanalyzer 2100. Nextera library preparation kit was used for DNA sample preparation. Briefly, one g high quality gDNA was subjected to fragmentation by Covaris shearing to obtain a final library with average insert size of 300 to 400 bp. Sheared DNA was then subjected to end repairing, adenylation of 3 ends followed by ligation of indexed paired end adapters. Ligated products with size range of 400-500 bp were purified using Qiagen MinElute gel extraction kit and selectively enriched by PCR. The libraries were validated for correct fragment size and concentration and then pooled for sequencing by Illumina HiSeq 2000 NGS platform.