Illumina HiSeq 2500 paired end sequencing; Roles of mRNA decay in liver development
Roles of mRNA decay in liver development
Mice were maintained on a 12 hr light / dark cycle in a temperature-controlled (22ºC) barrier facility with free access to water and a normal diet (NCD, CA-1, CLEA Japan, Inc.).  Isogen II extraction of total RNA was performed according to the manufacturer's instructions. 1 µg of total RNA was used for RNA-seq library preparation with TruSeq Stranded mRNA LT Sample Prep Kit (Illumina), according to the manufacturer's protocol.
Experimental Factor: phenotype
liver-specific Cnot3 knockout