454 GS FLX sequencing; Equine RNAseq Tissue pool
Equine RNAseq Tissue pool
RNA was isolated using variations of guanidinium-based protocols, MacLeod JN et al. (1996) J Biol Chem 271: 18954-18960.and Chomczynski P, and Sacchi N (1987) Anal Biochem 162: 156-159 messenger RNA was isolated from total RNA with the Oligotex kit (Qiagen, Valencia, CA). Primary cDNA library with adaptors compatible with the 454 system constructed as previously published Lambert JD et al (2010) Integr Comp Biol 50: 768-777.