GSM1876026: HCASMC_ATAC_TGFB_D2; Homo sapiens; OTHER
5x10^4 fresh HCASMC were collected by centrifugation and and nuclei extracted with cold lysis buffer containting 10mM Tris-HCl, pH7.4, 10mM NaCl, 3mM MgCl2, 0.1% IGEPAL and nuclei were resuspended in transposition reaction buffer containing Tn5 transposases (Illumina Nextera). Transposition reactions were incubated at 37C for 30 minutes, followed by DNA purification using Zymo DNA Clean-up and Concentration Kit. Libraries were PCR amplified and purified as described previously. The quality of the library preparation was determined by evaluation of the electropherogram traces from an Agilent 2100 Bioanalyzer DNA High Sensitivity and TBE gel electrophoresis, and libraries demonstrating appropriate nucleosomal enrichment were multiplexed. Libraries were quantitated using Qubit high sensitivity DNA reagent, and quantitative PCR (KAPA Biosystems).
GEO Accession
GSM1876026