Ixodes ricinus hemocyte Illumina data
The RNA Libraries of 300 bp prepared from total RNA according the manufacturer’s instructions “TrueSeq RNA Sample Prep Guide”. Protocol fragments the RNA using the Covaris S2 system, synthesize first and second strand cDNA, converts the overhangs resulting from fragmentation into blunt ends, adds an A-base to the 3' end of the blunt phosphorylated cDNA fragments and ligates adapters to the ends of the cDNA fragments. After PCR Enrichment of cDNA Fragments, Purification with AMPure beads XP and QC on Bioanalyzer/Qbit templates go on the cluster generation platform. The Illumina Cluster Station hybridized the fragments onto the flowcell and amplified them for sequencing on the Genome Analyzer IIx . The Genome Analyzer sequenced clustered template DNA using a robust four-color DNA Sequencing-By-Synthesis (SBS) technology. The fluorescent images were processed to sequences using the Illumina Genome Analyzer Pipeline Analysis software 1.8.