Whole genome sequence of S. brachiariae
DNA was randomly sheared to fragments with an average size of 20 kb by using Covaris g-TUBE. DNA damage and end of sheared DNA was then repaired. SMRTbell templates were obtained by ligating the blunt hairpin adapters to the ends of the repaired fragments, followed by the addition of exonuclease to remove failed ligation products. Before annealing the sequencing primer and binding polymerase to SMRTbell templates, the quality of library was assessed by an Agilent 2100 Bioanalyzer High Sensitivity Kit.