GSM3143752: seb1-1_epe1KO_tfs1DN_2; Schizosaccharomyces pombe; ChIP-Seq
ChIP DNA was extracted by bead beater with 0.5mm zirconia beads.  ChIP DNa was isolated by antibodies directed to our protein of interest (H3K9me2 or rpb3x-FLAG).  DNA was then isolated by incubation by SDS/proteinase K followed by column purification (Macherey-Nagel Nucleospin Gel Cleanup Columns). Libraries were prepared using End-It DNA End Repair Kit (Epicenter) followed by A-tailing using Klenow fragment (NEB), ligation to Illumina adaptors using Rapid T4 DNA Ligase (Enzymatics), PCR amplification for 15 cycles, and size selection between 200-350bp by bead purification and gel extraction.
GEO Accession
GSM3143752