GSM2058668: HCT116_wt_Top1_Seq_mod; Homo sapiens; OTHER
Cell were treated with CPT for 4 minutes and quickly incubated in TE-SDS 0.1%. Samples were sonicated to produce chromatin fragments of 300 bp on average. After clarification by centrifugation, sonicated extracts were adjusted to the conditions of FA_lysis buffer (50 mM HEPES, 150 mM NaCl, 2 mM EDTA pH 8.0 with 0.1% SDS). Standard ChIP was performed as previously described (Venters and Pugh, 2009). Samples were immunoprecipitated as described for ChIP, treated with tyrosyl-DNA phosphodiesterase 1 (TDP1), poly-nucleotide kinase (PNK) and Illumina sequenced.
GEO Accession
GSM2058668