Trimastix-transcriptome-2012
Sequencing library optimized for Roche/454 Titanium sequencing was prepared using GS FLX Titanium Rapid Library Preparation Kit from double-stranded cDNA. Fragment library was titrated by enrichment and prepared for sequencing by emulsion PCR on two regions of a two-region GS-FLX Titanium PicoTitreTM plate. The reads were cleaned of all adaptor/primer and polyA sequence. NEWBLER (v2.6; Roche/454 Sequencing) with the default parameters (40?bp overlap; 90% identity)was used for the assembly of 644 537 reads (average length 399 bp).