GSM4551242: Disease Stretched TPC Hashes; synthetic construct; RNA-Seq
Peritendous connective tissue was removed. Tendon tissue was then broken down by digestion with 0.1% type IV collagenase at 37°C with gentle agitation. Cells were then passed through a 70-micron filter to obtain a single cell suspension. Drop-seq libraries were constructed as detailed in Macosko et al. 2015 with minor exceptions. A glass dolomite chip was used instead of the traditional PDMS chip. 10X Chromium Single Cell 3' V3 libraries were generated according to the standard protocol by the Stanford Functional Genomics Facility (SFGF). Cell Hashing libraries were generated according to the standard protocol by the SFGF.
GEO Accession
GSM4551242