RAD Sequencing of Cepaea nemoralis cross C100 x C101: F1 yellow-unbanded #11
1) Cut genomic DNA from each sample with SbfI. 2) Ligate a modified Illumina adapter containing a TGCA overhang and a 5 base barcode, followed by pooling of individual samples. 3) Randomly shear fragments and size select to 300-600 bases. 4) Ligate a paired-end adapter. 5) Size select to 400-700 bases (range shift accounts for adapters). 6) Illumina sequencing.