GSM4119269: iBAT ko3; Mus musculus; RNA-Seq
Interscapular brown adipose tissues were removed, flash frozen on liquid nitrogen, and RNA was harvested using Isogen (Wako). Total RNA (100 ng) were used for RNA-seq library preparation with a TruSeq Stranded mRNA Library Prep Kit for NeoPrep (Illumina, NP-202-1001) that allows polyA-oligo(dT)-based purification of mRNA, according to the manufacturer's protocol with minor modification and optimization as follows. Custom dual index adaptors were ligated at the 5' and 3'-ends of library, and PCR was performed for 11 cycles. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM4119269