GSM4669492: AZ22_TSB1_Biofilm; Staphylococcus epidermidis; RNA-Seq
Total RNA was isolated from the mature biofilms of S. epidermidis strains grown in TSBg or PCs using FastRNA Pro Blue (MP Biomedicals, Irvine, CA, US). All RNA samples were treated with AmbionTM DNase I (Thermo Fisher Scientific) to digest genomic DNA. Samples exposed to PCs were further treated to remove contaminating eukaryotic RNA using MICROBEEnrichTM kit (Ambion). Ribosomal RNA (rRNA) was removed using Ribo-ZeroTM Meta-Bacteria kit (Epicentre) and strand-specific sequencing libraries were constructed using TruSeq kits (Epicentre). The generation of the cluster was performed using standard Illumina protocols with sequencing performed using an Illumina HiSeq 2500 sequencer from paired-end reads with a target read length of 100 nucleotides.
GEO Accession
GSM4669492