GSM4550189: N1570_doxycycline control 2; Homo sapiens; RNA-Seq
iPSCs were harvested and RNA were isolated with miRNeasy mini kit (Qiagen). 500ng of total RNA was used for mRNA enrichment procedure with NEBNext PolyA mRNA magnetic Isolation Module. Stranded cDNA library was generated using using NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina with an additional purification step using Agencourt® AMPure® XP Beads. cDNA libraries were barcoded by dual indexing approach with NEBNext Multiplex Oligos for Illumina – 96 Unique Dual Index Primer Pairs.
GEO Accession
GSM4550189