Human nasal 16S rRNA: Sample M9640936
Total genomic DNA was extracted using the PowerMag Microbiome DNA isolation kit.  The V4 region of the 16S rRNA gene was amplified by PCR using modified 515F and R806 primers that additionally contained adapters for MiSeq sequencing and a single 12-mer index barcode (R).  Individual libraries constructed from each sample were pooled and sequenced on the Illumina MiSeq platform using the 2x250 bp paired-end read protocol.