GSM3901019: BEAS2B: Braf_WT_1; Homo sapiens; RNA-Seq
RNA was isolated using the Rneasy Plus Mini Kit. Samples were checked using BioA and libraries were prepared using the TruSeq Stranded mRNA kit. Each library was quantified using Qubit and then pooled toether. Library pool was quantified using qPCR. Pool was then diluted to 4 nM and loaded on the HiSeq High Output Mode. Run was performed using a full flow cell (8 lanes) PE 100. 5% PhiX was spiked in to check run qaulity.
GEO Accession
GSM3901019