GSM3681644: SDK_Polysome_RNA_Sample_B; Homo sapiens; RNA-Seq
Red blood cells were isolated from whole blood. CD71 negative mature erythrocytes were isolated using CD71 magnetic beads based depletion. The cells were lysed using lysis buffer. RNA from this lysate was isolated using GeneJet RNA purification kit (Thermo Fisher Scientific) and corresponds to "Total RNA". For Polysome RNA, Toal erythrocytes were lysed using polysome buffer containing 2mM Dithiothreitol, 100μg/mL Cycloheximide, and 1% Triton X-100. The lysate was loaded on a sucrose gradient (10%-50%) and ultracentrifuged at 29000 RPM for 4 hours. The RNA from heavier fractions were isolated using the same kit as mentioned above and this RNA corresponds to "Polysome RNA". The RNA library was prepared according to the protocol for Illumina sequencing
GEO Accession
GSM3681644