GSM4217566: ChIP-exo_0hr_YY1; Mus musculus; ChIP-Seq
Cells are lysed, the nuclear fraction retained, and the chromatin is fragmented by sonication to 100-500bp. In vivo protein-DNA interactions are preserved through formaldehyde crosslinking. Cells are lysed, the nuclear fraction retained, and the chromatin is fragmented by sonication to 100-500bp. DNA fragments interacting with the protein of interest are enriched by ChIP, the important lambda exonuclease digestion effectively footprinting the left and right 5' DNA borders of the protein-DNA crosslink site, and a library is prepared by adding sequencing adapters according to manufacturer's instructions. ChIP-exo enriched DNA fragments are sequenced from the left and right 5' DNA borders of the protein-DNA crosslink site.
GEO Accession
GSM4217566