GSM4668353: MA-podo-exo; Mus musculus; miRNA-Seq
Exosomes were isolated from the cell culture supernatants using ExoQuick-TC Exosome Precipitation Solution (System Biosciences) in accordance with the manufacturer's recommendations, and exosomal RNA was extracted by the MagZol (Magen) according to the manufacturer's protocol. The quantity and integrity of exosomal RNA yield was assessed by using the Agilent 2200 TapeStation (Agilent Technologies, USA). RNAs were ligated with 3'RNA adapter, and followed by 5'adapter ligation. Subsequently, the adapter-ligated RNAs were subjected to RT-PCR and amplified with a low-cycle. Then the PCR products were size selected by PAGE gel according to instructions of NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Illumina, USA).
GEO Accession
GSM4668353