16S RNA-gene sequences of epiphytic bacteria: on Hydrilla verticillata in Guangfu bay of Taihu in May, sample 2
The representative leaves of three replicates of each species were collected by hand with gloves or using stainless hook and stored in aseptic plastic bag.After the plant samples were taken into lab, the epiphytic bacteria on leaves of each replicate were detached by an ultrasonic bath method. The DNA of detached bacterial biomass was extracted using the method of a modified phenol-chloroform method. The V1每V3 region of bacterial 16S rRNA genes was amplified and The purified PCR products were combined at equimolar ratios and then run on a Roche 454 FLX pyrosequencing machine.