Illumina HiSeq 2000 sequencing; High-througput sequencing of the rtel1 mutant
High-througput sequencing of the rtel1 mutant
The rtel1 knockout (rtel1-1) and the Col-0 seeds were germinated on control 1/2MS medium on a nylon mesh. Root tips (less than 2 to 3 mm) were collected and frozen in liquid nitrogen. RNA was extracted from root tissue with the RNeasy plant kit (Qiagen). Illumina True-Seq libraries were generated from cDNA according to the manufacturers protocol and sequenced on a HiSeq2000.
Experimental Factor: genotype
wild type genotype