GSM2817010: Patients_13; Homo sapiens; miRNA-Seq
blood samples were collected in EDTA tubes and centrifuged at 2500 rpm for 10 minutes. Once plasma was obtained, each sample was stored at −80°C until RNA extraction. 500 µL of plasma were used to isolate cell-free total RNA (including miRNAs) using the miRNeasy Serum/Plasma kit (Qiagen, Valencia, CA, USA) following the manufacturer's protocol. RNA libraries were prepared with NEBNext Multiplex Small RNA Library Prep Set for Illumina (Set 1&2), and they were sequenced using standard Illumina protocols.
GEO Accession
GSM2817010