GSM3143257: Young rep1; Homo sapiens; OTHER
cfDNA was isolated from 3 to 4 mL of plasma using the QIAamp Circulating Nucleic Acid Kit (Qiagen, Hilden, Germany). At least 20 ng of total DNA from each sample was extracted and quantified using Qubit fluorometer (Thermo Fisher Scientific, Waltham, MA, USA). Sequencing libraries were prepared from the purified DNA using Ovation® Ultralow Library Systems V2 library preparation kit (NuGen Technologies, San Carlos, CA, USA). Sequencing was performed on HiseqX-Ten Illumina platform at BGI Beijing Genomics Institute, Hong Kong.
GEO Accession
GSM3143257