PacBio DNA-seq of Bacteroides fragilis: BF_1985
DNA extraction with Qiagen Genomic Tip g/500. Library was prepared using Pacific Biosciences protocol for SMRTbell Libraries using PacBio Barcoded Adapters for Multiplex SMRT Sequencing. DNA was sheared to 10 kb fragments using g-tubes from Covaris. Samples were pooled during library prep using equal mass, eight samples in each library. Final library was size selected using 0.45x Ampure PB beads. The library was sequenced on Pacific Biosciences Sequel instrument using Sequel Polymerase v2.0 and Sequencing chemistry v2.10. Loading was performed by diffusion, one SMRT cell was sequenced. Movie time: 600 min, pre-extension time: 120 min. Reads were demultiplexed using Barcoding pipeline on SMRT Link (5.1.0.26412, SMRT Link Analysis Services and GUI 5.1.0.26411) using default settings with minimum barcode score 40.