Illumina HiSeq 3000 paired end sequencing; Total RNA-seq of human oesophageal adenocarcinoma cell line OE19 treated with siNT, siHNF4A, siGATA6 and siHNF4A+siGATA6 for 72 hours
Total RNA-seq of human oesophageal adenocarcinoma cell line OE19 treated with siNT, siHNF4A, siGATA6 and siHNF4A+siGATA6 for 72 hours
Cells were cultured in 37deg incubator with 5% CO2. RNA was collected using Qiagen RNeasy kit. Cells were lysed directly in the plate.  RNA-seq libraries were constructed using Illumina TruSeq Stranded mRNA kit.
Experimental Factor: RNA interference
siRNA knockdown of HNF4A