GSM2813717: ID8_2; Mus musculus; OTHER
Gentra Puregene Digital restriction enzyme analysis of methylation (DREAM) was performed to determine the methylation profile of ID8 mouse ovarian cancer cell line. Briefly, genomic DNA spiked with methylation standards was sequentially digested by SmaI and XmaI, which both recognize the sequence CCCGGG. SmaI is methylation sensitive, where XmaI is methylation insensitive. Distinct signatures, 5'-GGG at unmethylated sites or 5'-CCGGG at methylated sites were created by enzyme digestion. 3' recesses were filled in and Illumina adaptors were ligated to the ends of restriction fragments. Barcoded library pools were sequenced using Illumina HiSeq2500 instrument.
GEO Accession
GSM2813717