MaxiIB-Nano
Nanopore sequencing was conducted in our lab. Briefly, genomic DNA was fragmented to 20 kb using g-Tubes (Covaris, USA), according to manufacturer instructions and libraries were prepared with the library preparation kit (EXP-NBD103/SQK-LSK108, Nanopore, England), starting from 1 g of total fragmented genomic DNA. Libraries were sequenced 20 hours in a R9.4 FlowCell (FLO-MIN106, Nanopore, England).