16S profilinig of 16S_heating_effect_obesogenic_diet
This sample contains the raw data of 16S rRNA gene sequencing from feces of Wistar rats treted with six different diets: Low Fat Diet (LFT), Heated Low Fat Diet (Heated-LFD), High Fat Diet (HFD), Heated High Fat Diet (Heated-HFD), Western Diet (WD), Heated Western Diet (Heated-WD). The samples/barcodes are: WD_24R/TGGAGGACGGAC, WD_30R/CTGACATAATC, WD_36R/AGCACGAATC, Heated_HFD_39R/TTGGAGGCCAGC, HFD_40R/TGGAGCTTCCTC, Heated_WD_41R/TCAGTCCGAAC, WD_42R/TAAGGCAACCAC, Heated_LFD_01/CTAAGGTAAC, LFD_02/TAAGGAGAAC, Heated_LFD_06/TACCAAGATC, LFD_07/CAGAAGGAAC, Heated_LFD_11/TTCGTGATTC, LFD_12/TTCCGATAAC, Heated_LFD_16/TGAGCGGAAC, LFD_17/CTGACCGAAC, Heated_HFD_21/TAGGTGGTTC, HFD_22/TCTAACGGAC, Heated_WD_23/TTGGAGTGTC, Heated_HFD_27/TCTGGATGAC, HFD_28/TCTATTCGTC, Heated_WD_29/AGGCAATTGC, HFD_34/CAGATCCATC, Heated_WD_35/TCGCAATTAC, Heated_HFD_33/AACCTCATTC. The primer sequence for the mapping file is GATGTGCCAGCNGCCGCGGTAA. The products were verified through electrophoresis in an agarose gel, purified with AMPure XP PCR Purification Kit (Beckman Coulter), quantified using Qubit dsDNA HS Assay Kit (Invitrogen) and subjected to emulsion PCR using Ion PGM Hi-Q View OT2 Kit (Thermo Fisher Scientific). After, the resulting enriched beads were sequenced in a NGS machine (Ion Torrent PGM, Life Technologies) using Ion PGM Hi-Q View Sequencing Kit (Thermo Fisher Scientific).