Illumina HiSeq 2500 paired end sequencing; ATAC-seq of Oesophageal Cell Lines
ATAC-seq of Oesophageal Cell Lines
OE33 cells were grown in RPMI media supplemented with 10% foetal bovine serum, MFD1 and HET1A cells were grown in DMEM media supplemented with 10% foetal bovine serum. ATAC-seq was performed as published in Buenrostro, J. D., Giresi, P. G., Zaba, L. C., Chang, H. Y., & Greenleaf, W. J. (2013). Transposition of native chromatin for fast and sensitive epigenomic profiling of open chromatin, DNA-binding proteins and nucleosome position. Nature Methods, 10(12), 1213-1218. http://doi.org/10.1038/nmeth.2688
Experimental Factor: HET1A
cell line