GSM3081869: st3_rep2; Parasteatoda tepidariorum; RNA-Seq
Poly(A) mRNA was extracted using the Dynabeads mRNA DIRECT Kit (Ambion) from 10-100 embryos of each successive developmental stage. mRNA was fragmented using the NEBNext Rnase III RNA Fragmentation Module (New England BioLabs). Sequencing libraries were constructed from fragmented RNA using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs) and NEBNext Multiplex Oligos for Illumina (Index Primers Set 1, New England BioLabs).
GEO Accession
GSM3081869