GSM1611458: WHIM12 ZNF165 Input; Homo sapiens; ChIP-Seq
Nuclei from WHIM12 cells stably expressing control or ZNF165-V5 were isolated, sonicated and immunopreciptated with V5 antibody. 5 ng of ChIP-ed DNA underwent library preparation using the KAPA HTP Library Preparation Kit. ChIP-ed DNA was end repaired, 3’ end adenylated and barcoded with multiplex adaptors. Ampure XP beads were used to purify DNA. Library size was assessed using the Agilent 2100 Bioanalyzer. The Invitrogen Qubit® Fluorometer was then used to quantify samples prior to normalizing and pooling.
GEO Accession
GSM1611458