GSM2475810: OK Illumina RNASeq; Didelphis virginiana; RNA-Seq
Cells were collected using Accutase (Sigma) and RNA was extracted using the ambion PureLink RNA mini kit (ThermoFisher) according to the manufacturer’s protocol. Ribosomal RNA sequences were removed before library construction. Library preparation was performed using the TruSEQ Stranded Total RNA Sample Preparation Kit (Illumina) according to manufacturer’s instructions. RNA was fragmented for 8 minutes and reverse transcription performed. Double stranded cDNA was subjected to 3’ adenylation and ligation of sequencing adapters. Sequencing was carried out on a NextSeq 500 (Illumina) for 2 x 75 bp paired end reads. Loading concentration was 1.6 pM.
GEO Accession
GSM2475810