Strand-specific RNA-Seq of wheat spikelets after 0h post-inoculation of Fusarium graminearum (biological replicate 1)
Total RNAs were extracted with TRIzol (Invitrogen, USA) and treated with RNase-free DNase I. Poly(A)+ mRNA was isolated with the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Strand-specific RNA-seq libraries were constructed with the NEBNext Ultra Directional RNA Library Prep Kit (New England Biolabs).