Illumina MiSeq paired end sequencing
DNA amplification control soil
Three independent PCR amplifications targeting different genes were conducted. Amplicons producted were then pooled for each sample (i.e, 1 sample with three different amplicons). Library was then prepared using NEB kit NEBNext ultraII DNA (96 reactions) and NEB dual index multiplex oligos. There is 3 different insert sizes (424, 530, 940) in each sample since three pairs of primers were used and amplicons were then pooled. Three  forward md5: Bacteria (5'==>3') 16S V4-V7 799f: AACMGGATTAGATACCCKG, Fungi (5'==> 3')  SSU_817 F: TTAGCATGGAATAATRRAATAGGA, Archaea (5'==> 3') AR_20F ACACTGACGACATGGTTCTACATTCCGGTTGATCCYGCCRG. Three reverse md5: Bacteria (5'==>3') 16S V4-V7 1223r: CCATTGTAGTACGTGTGTA, Fungi (5'==> 3')   ns22bR: AATTAAGCAGACAAATCACT, Archaea (5'==> 3') AR_958R TACGGTAGCAGAGACTTGGTCTYCCGGCGTTGAMTCCAATT