16S sequencing of mouse gut microbiome
Microbial community composition was assessed by pyrosequencing 16S rRNA genes derived from ceca of mice fed a normal chow (n = 11) or L-carnitine (n = 13) diet. DNA was isolated using the MoBio PowerSoil DNA Isolation Kit. The V4 region of the 16S ribosomal DNA gene was amplified using bar-coded fusion primers (F515/R806) with the 454 A Titanium sequencing adaptor.