GSM1692698: MF4; Homo sapiens; ncRNA-Seq
Total RNA was extracted using Trizol. Disposable RNA chips (Agilent RNA 6000 Nano LabChip kit; Agilent Technologies, Waldbrunn, Germany, http://www.home.agilent.com) were used to determine concentration and purity/integrity of RNA with Agilent 2100 Bioanalyzer. For each sample, a small RNA library was prepared starting from 1 ug total RNA, using the TruSeq Small RNA Sample Preparation Kits and protocols (Illumina, San Diego, CA, USA, http://www.illumina.com). Library quality was checked using High Sensitivity DNA chip (Agilent Technologies, Waldbrunn, Germany, http://www.home.agilent.com).
GEO Accession
GSM1692698