GSM2474549: P18_3; Monodelphis domestica; RNA-Seq
Samples were cryosectioned to collect tissue for RNASeq. N = 3 specimens were collected for each stage (16, 18 and 20 day) and an Arcturus Veritas Microdissection Instrument was used to laser capture microdissect (LCM) the MC and malleus (with minimal surrounding perichondrium) focusing on their connection area. RNA isolation was completed using an Arcturus PicoPure RNA Isolation Kit, and the Clontech SMARTer Ultra Low Input RNA Kit for RNA amplification. RNA-Seq Libraries were constructed using a Nextera XT DNA Sample Preparation Kit, along with a Nextera XT Index Kit and TruSeq Dual Index Sequencing Primers.
GEO Accession
GSM2474549