GSM1608263: ERSrcEtOH24hr_rna; Homo sapiens; RNA-Seq
For RNA-seq, total RNA was purified from DNase-treated lysates, and ribosomal RNA was depleted with RiboMinus Eukaryote Kit (Invitrogen). RNA-seq libraries were prepared with a tagging-based workflow(Pease and Kinross, 2013). In brief, rRNA-depleted RNA was fragmented at 85°C for 5 min, followed by cDNA synthesis, terminal tagging and PCR amplification with ScriptSeq v2 RNA-Seq Library Preparation Kit (Epicentre).
GEO Accession
GSM1608263