GSM3477214: TARDBP eCLIP, replicate 1; Homo sapiens; RIP-Seq
Cell pellets were lysed in 1 mL lysis buffer, followed by RNAse I digestion and immunoprecipitation with 10 µg of anti-TDP-43 antibody. Barcoded RNA adapter was ligated to the 3′ end, run on standard protein gels and transferred to nitrocellulose membranes. A region 75 kDa (~150 nt of RNA) above the protein size was isolated and treated with proteinase K. RNA was reverse transcribed and treated with ExoSAP-IT. A second DNA adapter was then ligated to the cDNA fragment 3′ end, and the products were PCR-amplified to generate library for sequencing.
GEO Accession
GSM3477214