RNA-Seq Analysis Implicates the Polycyclic Aromatic Hydrocarbon-Degrading Pathways in Rhodococcus aetherivorans IcdP1
Rhodococcus sp. strain IcdP1 was isolated from coking chemicals-contaminated soil. Biodegradation tests showed that it was able to mineralize or degrade numerous PAHs. To understand the molecular mechanisms of PAHs degradation in the IcdP1, we employed RNA-seq to compare and characterize the differentially expressed genes in pure culture exposed to indeno[1,2,3-cd]pyrene and noninduced. RNA was extracted and purified. rRNA was romeved by Ribo-Zero Magnetic Kit. A paired-end library (200bp) was constructed and sequenced 101bp from both ends by Hiseq2500.