GSM4669219: hyp.1: G variant of p53 from subject c2, untreated, replicate 1; Homo sapiens; RNA-Seq
Total RNA was extracted from MCF10A cells after 24 h of co-culture with MCF7 cell-derived sEV using Direct-zol RNA MiniPrep kit (Zymo Research). RNA quantity was determined using the Qubit 2.0 Fluorometer (ThermoFisher Scientific) and the quality was validated using the TapeStation RNA ScreenTape (Agilent). One-hundred ng of total RNA was used to prepare a library for Illumina Sequencing using the Quant-Seq 3'mRNA-Seq Library Preparation Kit (Lexogen)
GEO Accession
GSM4669219