GSM1689797: insituDNaseHiC-WG-patski-rep2; Mus musculus x Mus spretus; OTHER
DNase Hi-C or in situ DNase Hi-C:  Cells are cross-linked with formaldehyde; chromatin is then randomly fragmented by DNase I. The resulting chromatin fragments are end-repaired and dA-tailed, then marked with a biotinylated internal adaptor; and proximity ligation is carried out inside individual nuclei to favor ligation events between the cross-linked DNA fragments. The resulting DNA sample contains ligation products consisting of chimeric DNA fragments that were originally in close spatial proximity in the nucleus, marked with biotin at the junction. A whole-genome chromatin interaction library is created by shearing the DNA and selecting the biotin-containing fragments with streptavidin magnetic beads. After linear library amplification, a DNase Hi-C library is generated and can be sequenced to identify whole-genome chromatin contacts.
GEO Accession
GSM1689797