GSM2054963: psh1∆KAN Rad5+ t2 batch 2; Saccharomyces cerevisiae; RNA-Seq
RNA was extracted using the Acid-Phenol RNA extraction methods, followed by DNAseI treatment (Invitrogen, Amplification Grade) and phenol:chloroform extraction following by ethanol precipitation. Total RNA integrity was checked using an Agilent 2200 TapeStation (Agilent Technologies, Inc., Santa Clara, CA) and quantified using a Trinean DropSense96 spectrophotometer (Caliper Life Sciences, Hopkinton, MA).  RNA-seq libraries were prepared from total RNA using the TruSeq RNA Sample Prep v2 Kit (Illumina, Inc., San Diego, CA, USA) and a Sciclone  NGSx Workstation (PerkinElmer, Waltham, MA, USA).  Library size distributions were validated using an Agilent 2200 TapeStation (Agilent Technologies, Santa Clara, CA, USA).  Additional library QC, blending of pooled indexed libraries, and cluster optimization were performed using Life Technologies' Invitrogen Qubit® 2.0 Fluorometer (Life Technologies-Invitrogen, Carlsbad, CA, USA).  RNA-seq libraries were pooled (18-plex) and clustered onto a flow cell lane.
GEO Accession
GSM2054963