GSM3143223: WT_Rep1; Mus musculus; RNA-Seq
RNA extraction was performed by Nugen's Ovation RNA-seq System V2 We use poly-A pull-down to enrich mRNAs from total RNA samples (200ng-1ug per sample, RIN>8 required) and proceed on library preparation by using Illumina TruSeq RNA prep kit. Libraries are then sequenced using Illumina HiSeq2500 at Columbia Genome Center. We multiplex samples in each lane, which yields targeted number of single-end/paired-end 100bp reads for each sample, as a fraction of 280-400 million reads for the whole lane.
GEO Accession
GSM3143223