Fragment library Holstein cow
Fragment library was created from 10 µg of genomic DNA, which was sheared into fragments with a mean size of 160 bp using Covaris S2 system (Covaris, MA, USA). Fragments were then end-repaired and P1 and P2 adaptors ligated. Purified fragments with adaptors were further analysed on an E-Gel 2% Size-Select gel (Invitrogen, ON, Canada) and 150-200 bp fraction was collected.