Raw whole genome sequence of Aspergillus fumigatus isolated from a patient.
Fungal mass was suspended in TE buffer (pH 8, 1% SDS, 2% Triton X100, 100mM NaCl). The suspension was shaken for 30 min at 70 C. DNA was extracted using phenol/chloroform extraction and purified using the QIAamp DNA Blood Mini kit (Qiagen, Germany). A fragmented genomic DNA library was prepared using a Nextera XT DNA sample preparation kit (Illumina, USA). Subsequent sequencing was conducted in a paired end 2150 bp mode using an Illumina NextSeq 500 machine (Illumina, USA).