GSM3602288: WGBS_RPCs_P3; Mus musculus; Bisulfite-Seq
Genomic DNA was purified from the samples using the DNeasy Blood & Tissue Kit (QIAGEN #69504). DNA concentration was measured using the Qubit® dsDNA BR Assay Kit (Thermo Fisher Scientific). The DNA quality of the samples was assessed with the Fragment AnalyzerTM and the DNF-487 Standard Sensitivity genomic DNA Analysis Kit (Advanced Analytical). Approximately 5 μg of genomic DNA per sample spiked with lambda DNA was fragmented by sonication to 200–400 bp using Covaris S220 followed by end repair and adenylation. Cytosine-methylated barcodes were ligated to sonicated DNA, and then these fragments were treated with bisulfite (EZ DNA Methylation-GoldTM Kit, Zymo Research). The resulting single-stranded DNA fragments were amplified by PCR using KAPA HiFi HotStart Uracil + ReadyMix (2×). DNA concentration of the libraries was measured using the Qubit® dsDNA HS Assay Kit (Thermo Fisher Scientific). The library profiles were checked using the High Sensitivity DNA chip for the 2100 Bioanalyzer (Agilent). WGBS libraries were sequenced on a HiSeq X-10 platform using 150 bp paired-end sequencing.
GEO Accession
GSM3602288