Short RNA-seq of Homo Sapien: adult male LCL
Cells were grown in suspension using RPMI 1640 media with 30%                 non-heat inactivated FBS + glutamate (Sigma-Aldrich). After seeding, cells were                 grown for 3-5 days and harvested. Total RNA was isolated using Trizol extraction                 (Invitrogen). RNA library was prepared for sequencing using standard Illumina TruSeq                 cDNA library preparation protocols. The Illumina NextSeq 3'-adapter is                 TGGAATTCTCGGGTGCCAAGG. Short RNA-Seq was run on the Illumina NextSeq 500 sequencer.