GSM3603189: SIX1_SIX2_KO_H1792_1; Homo sapiens; RNA-Seq
Total RNA purification was performed according to RNAeasy mini kit instruction (QIAGEN, 74104), with DNA digestion step according to the kit instruction RNA concentration was determined with a Qubit fluorimeter prior to library prep.  4μg of total DNAse treated RNA were prepared with the TruSeq Stranded Total RNA LT Sample Prep Kit from Illumina. In brief, polyadenylated RNA was purified and fragmented before strand specific cDNA synthesis.  cDNA was A-tailed and indexed adapters were ligated. After adapter ligation, samples were PCR amplified and purified with AmpureXP beads, then validated again on the Agilent 2100 Bioanalyzer.
GEO Accession
GSM3603189