GSM3141138: PHBA8_L [leaf, miRNA-Seq]; Populus x canadensis; RNA-Seq
All samples were frozen in liquid nitrogen and stored at –80°C. Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's instructions. DNase I (TaKaRa, Dalian, China) was used to treat RNA samples to avoid genomic DNA contamination. The quantity and purity of the total RNA was measured with a Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) (RIN number >7.0). Equal parts of RNA samples for the same treatment were mixed for transcriptome sequencing and sRNA sequencing. Subsequently, the total mixed RNA sample for leaves was prepared for degradome sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM3141138