GSM2817091: E7onC5728dpg_3; Mus musculus; RNA-Seq
Whole ear samples and grafts were collected from mice euthanized with 5% CO2. Samples were collected into Eppendorf tubes and immediately snap frozen on dry ice. Samples were kept in the -80°C freezer until used for RNA extraction. All RNA extractions were performed using Trizol and Qiagen RNeasy mini kit (catalogue #74106, Qiagen, Clifton Hill, VIC, Australia)) following manufacturer's protocol. cDNA library preparation from RNA samples was performed using Low Throughput Sample Protocol (suitable for 48 samples or less) from Illumina TruSeq RNA Sample Preparation Kit v2 (catalogue #15027387, #15025063 and #15027084 obtained from Illumina, San Diego, CA, USA).
GEO Accession
GSM2817091