GSM2652179: Nup153_RbFLAG_1; Mus musculus; ChIP-Seq
Approximately 3 x106 cells were cross-linked with 2.5 mM DSG and 1% formaldehyde at room temperature for 15 min. After quenching with 125 mM glycine, samples were briefly sheared by sonication and the concentration of DNA was measured by Nanodrop. Twenty µg of chromatin were treated with Micrococcal Nuclease (1 U/µl, Worthington), and chromatin was incubated with antibodies at 4℃ overnight. Immunoprecipitated complexes were collected using Dynabeads conjugated with anti-rabbit IgG (Life Technologies). Subsequently, beads were washed and DNA was extracted and purified by Ampure XP beads (Beckman). A DNA library was generated using NEBNext Ultra DNA library prep kit for Illumina and Bioo Illumina barcodes followed by deep sequencing with the Illumina’s Hiseq2500 at SE50.
GEO Accession
GSM2652179