GSM4551942: P13_CTX_SSTcre_Sun1IP_BS_rep2; Mus musculus; Bisulfite-Seq
Mouse cortices were collected and DNA was extracted using Qiagen Blood and Tissue kit. WGBS libraries on INTACT-purified nuclei were generated by using a similar method as previously described (Stroud et al., 2017). Briefly, genomic DNA including unmethylated lambda DNA (0.5%; Promega D1521) were sonicated to 200bp using a covaries S2 or M220, and NEXTflex methylated adapters (Bioo Scientific) were ligated onto the sonicated DNA. The libraries were bisulfite-treated using the EZ DNA methylation-Gold kit (Zymo). The final libraries were sequenced on a Nextseq 500.
GEO Accession
GSM4551942