RNA-Seq of Flaveria leaf development
ibraries were prepared with the TruSeq RNA Library Prep Kit v2 (Illumina) using 0.5 g of total RNA as a starting material. Quality and quantity of the libraries were determined with the 2100 Bioanalyzer and a Qubit instrument (Thermo Fisher Scientific), respectively, according to the instructions given by the manufacturer. Single end reads of 100 bp length were sequenced with the HiSeq2000 Illumina platform and samples were multiplexed with 6 libraries per lane. On an average ~ 33 million reads were obtained per sample.