CENP-A (Cnp1)
CENP-A (Cnp1)
The samples were incubated for 4-5 h with 10 ul -Cnp1-antiserum [41] and 40 ul Protein G agarose beads (Roche). The beads were washed as described and incubated with 1 per cent SDS overnight at 65 C. The supernatant was purified using a PCR purification kit (Qiagen).