Sample from Rumen microbiome inoculum_01
The rumen sample “inoculum 01” was collected by an esophageal probe, 2 h before morning feeding from 3 dry Holstein-Friesian cows housed at the experimental farm of the University of Padova (Italy). Animals were fed hay ad libitum with 2.5 kg/d of concentrates (0.5 kg of dry sugar beet pulp, 1 kg of corn grain, and 1 kg of sunflower meal). Metagenomic DNA was extracted Genomic DNA was extracted from supernatant using the RNA PowerSoil® DNA Elution Accessory Kit (MO BIO laboratories, Carlsbad, CA). Libraries were prepared using Nextera DNA Library Preparation Kit (Illumina, San Diego, CA) and paired-end sequenced using Illumina Hi Seq 2500.