GSM4550137: RelA 30 rep1; Escherichia coli; OTHER
The cells were subsequently UV-C crosslinked and harvested by centrifugation, washed in ice-cold phosphate buffered saline (PBS) and snap frozen in liquid nitrogen. The pellets were stored at -80C. The HTF tagged proteins were then purified, linkers ligated to crosslinked RNA, cDNA synthesized and DNA libraries generated as described in Winther et al. 2018. Libraries were constructed as per, Sinha and Winther 2020
GEO Accession
GSM4550137