Exome capture of bulked segregant pooled tissue of crosses between Hordeum vulgare powdery mildew susceptible mutant m11526 (Mla6) and two diverse resistant lines, P03 (Pallas, Mla6) and Sultan-5 (Mla12)
Two-hundred 7-day old F2 seedlings from both m11526 x P03 and m11526 x Sultan-5 populations (400 F2 in total) were inoculated with Bgh 5874, infection phenotyped 7 days after inoculation (DAI),  and then treated with fungicide.  Tissue was harvested from 3rd leaves at 21 days old, into groups of 10 F2 seedlings per standard 2x CTAB DNA extraction.  The grouped gDNA was then pooled for a total of four population pools; 150 resistant P03-derived, 50 susceptible P03-derived, 150 resistant Sultan-5-derived and 50 susceptible Sultan-5-derived.  Samples were processed for exome capture according to the SeqCap EZ Library SR User Guide (Roche NimbleGen, Version 5.1, Barley Exome Custom Sequence Capture design, 06740278001) using the KAPA HyperPrep Kit (KK8500) and sequenced on a single lane of an Illumina HiSeq 3000.