GSM3478016: 72 hpf retina; Danio rerio; RNA-Seq
We dissected 24-, 36-, 48-, 72-hpf retinas of wild-type zebrafishes and isolated via papin solution. 14-dpf retinas were dissected from PCNA-GFP transgenic fishes. And after isolation by papin solution, the GFP positive cells which could label  the ciliary marginal zone (CMZ) cells were sorted via FACS. These isolated cells filtered by 40μm cell strainer (BD Falcon) were loaded onto the Chromium Single Cell Chip(10x Genomics) according to the manufacturer's protocol. Single-cell RNA-seq libraries were generated using the GemCode Single-Cell Instrument and Single Cell 3' Library &Gel Bead kit v2 Chip kit (10x Genomics) following the manufacturer's protocol.
GEO Accession
GSM3478016