Illumina HiSeq 4000 sequencing; RNA-seq of human pancreatic cell lines, L3.6pl and BxPC-3, upon knockdown of p63 for 48 hours compared to siControl
RNA-seq of human pancreatic cell lines, L3.6pl and BxPC-3, upon knockdown of p63 for 48 hours compared to siControl
Cells were transfected with siControl and sip63 using Lipofectamine® RNAiMAX (Invitrogen) according to the manufacturer's instructions. Triplicates for each condition were harvested 48 hours after transfection by QIAzol® reagent (Qiagen) according to manufacturer's instructions. Reverse transcription of 1 µg RNA was performed using M-MuLV reverse transcriptase (NEB) and random primers. The quality of RNA from L3.6pl and BxPC-3 with a triplicate for siControl condition and sip63 were checked by loading on a 1% agarose gel. Libraries for RNA were prepared using the TruSeq RNA Library Prep Kit V2 (Illumina) according to the manufacturer's instructions.
Experimental Factor: cell line
BxPC-3
Experimental Factor: RNA interference
scrambled siRNA