GSM4217474: input RNA, senescent cells; Homo sapiens; OTHER
Total RNA was isolated from control or oncogenic RAS-induced senescent IMR90 cells 9 days post infection (three independent batches of cells were collected) using the RNeasy maxi kit (Qiagen, 75162) and digested with DNase I (Qiagen, 79254). polyA+ RNA was purified using the GenElute mRNA Miniprep kit (Sigma). Purified mRNA was fragmented using ZnCl2 buffer into ~100 nt and subjected to immunoprecipitation with an anti-m6A antibody (Synaptic Systems, Cat. No: 202 111). m6A-containing mRNAs eluted by free m6A and input fragments from control and senescent cells were used for library preparation
GEO Accession
GSM4217474