WXS human: lymph node prostate tumor metastasis: Sample WCMC161_1_C_WES_Tumor
DNA was extracted using Promega Maxwell 16 MDx. WXS capture libraries were constructed from tumor and normal tissue after sample shearing, end repair, and phosphorylation and ligation to barcoded sequencing adaptors. Ligated DNA was size selected for lengths between 200-350 bp and subjected to exonic hybrid capture.
alignment_software
BWA