Elucidating the gut microbial community of farmed pompano reared in sea-cages.
Library preparation was performed at Genotypic Technology’s Genomics facility. 1μl of DNA was used to generate ~500bp product from V3-V4 region of 16S DNA with specific primers which also have a ‘tag’ sequence that are complementary to primers in Nextera XT Index kit V2. The Amplicon was visualized on the Agarose Gel prior to Indexing PCR . The amplicon was further amplified for 6 PCR cycles using dual Indexing primers to obtain a final product of ~600bp. The library was purified using HighPrep PCR (Magbio, Cat # AC-60050) magnetic beads and was quantified using Qubit Fluorometer.The individual libraries will be pooled in equimolar ratio and loaded on MiSeq for sequencing.