GSM4669489: MMQ Ctrl 24h; Rattus norvegicus; RNA-Seq
Total RNAs from MMQ cells are isolated using TRIzol reagent (Invitrogen) and treated with RNase-free DNase I (New England Biolabs, MA, USA), to remove any contaminating genomic DNA. mRNA extraction is performed using Dynabeads oligo(dT) (Invitrogen Dynal).Double-stranded complementary DNAs are synthesized using Superscript II reverse transcriptase (Invitrogen) and random hexamer primers. The cDNAs are then fragmented by nebulization and the standard Illumina protocol is followed thereafter, to create the mRNA-seq library. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM4669489