GSM2651159: WT_2i_Gro_2; Mus musculus; OTHER
Cells were pelleted, washed twice with ice-cold PBS and kept on ice. Lysis buffer (10 mM Tris-HCl pH 8, 10 mM NaCl, 3 mM CaCl2, 2 mM MgCl2, 0.5 % NP40, 0.5 mM DTT, 300 mM sucrose, 1X protease inhibitor cocktail and SUPERase-In) was added and cells were pipetted up and down through 1 ml tips to release nuclei. The nuclei were washed twice with lysis buffer and resuspended in 100ul freezing buffer (50 mM Tris-HCl, pH 8.3, 40% glycerol, 5 mM MgCl2, 0.1mM EDTA, and 4 units of SUPERase-In per mL). 5 x 106 nuclei were used for each library. Nuclear run-on performed as previously described (Williams et al 2015) library construction were performed as previously described  (Franco et al., 2015)
GEO Accession
GSM2651159