Illumina HiSeq 2000 paired end sequencing; Allele-specific expression in liver, hypothalamus and vomeronasal organ of Mus.m.musculus and M.m. domesticus
Allele-specific expression in liver, hypothalamus and vomeronasal organ of Mus.m.musculus and M.m. domesticus
female offspring (3 to 6 individuals per genotype and cross direction   were sacrificed at the age of 11 to 13 weeks to prepare the HYP, the VNO, and the LIV from the same individuals. All dissections were done by the same person following personal instructions and standardized protocols designed by Valery Grinevich (MPI for Medical Research Heidelberg for HYP) and Masayo Omura (MPI for Biophysics, Frankfurt for VNO). For the HYP, brains were removed and further dissection was performed on wet ice using gross anatomical borders (i.e., optic chiasm arms, optic tracts, and mammillary body) as markers to remove the HYP. For the VNO, we followed the first steps of the procedure described in Meeks and Holy (2009). Prepared tissues were immediately frozen on dry ice and kept at minus 70 degree centigrade until RNA preparation Using TRIzol Reagent with the PureLink RNA Mini Kit, invitrogen/ambion With On-Column PureLink DNase Treatment during Purification Illumina TruSeq RNA Sample Preparation Kit v2
Experimental Factor: strain
cross M.m musulus (PWD/PhJ) and M. m. domesticus (WSB/EiJ)
Experimental Factor: organism part
liver
Experimental Factor: genotype
PWD/PhJ mother, WSB/EiJ father
LIBRARY_STRAND
not applicable