The microbial community
Amplification of the 16S rRNA gene for the production of clone libraries was performed using two pairs.For 5 min, 30 cycles of 94 1C for 1 min, 54 1C for 1 min, and 72 1C for 1 min, 72 1C for 7 min and 4 1C hold. PCR products were cloned using the TOPO TA Cloning Kit, following the manufacturers instructions. Individual clones were cultured and their plasmids purified using the QIAprep Spin Miniprep Kit. Inserts in each were sequenced from two ends using Big Dye Terminator chemistry and the Applied Biosystems 3100 Genetic Analyzer. Chimeric sequences were screened by the CHIMERA DETECTION program of the Ribosomal Database Project (RDP) and removed from further analysis.