Phased WGS of H. sapiens: Adjacent normal colon tissue
1 nanogram of purified genomic DNA was partitioned into over 100,000 microdroplets and pre-amplified with barcoded random primers using a beta version of the 10X GemCode instrument. The droplets were broken and purified, and the resulting DNA fragments were ultrasonically sheared. End repair and a-tailing reactions were performed and adapters containing the R2 sequencing primer, a sample index, and the P7 flowcell sequence were ligated to the sample. Libraries were then purified and amplified by PCR before sequencing.
alignment_software
10X pipeline