in vitro embryogenic tissues of P. abies (Norway Spruce) at the proliferation stage growing at different temperatures
Massive analysis of cDNA (MACE; GenXPro-GmbH) was used. Biotinlyted poly-A 3'ends of cDNA are randomly fragmented and sequenced from the 5' side of each end. Each transcript molecule is represented by one 94 bp tag derived from a region of 100-500 bps of the 3' end. GenXPro's "TrueQuant" method was used to eliminate PCR bias.