RNA from vesicles of A. laidlawii PG8 treated with melittin
Acholeplasma laidlawii cells were treated with melittin. The isolation of the A. laidlawii extracellular vesicles was performed on 3 biological replicates. The vesicles were treated by DNAse and RNAse. RNA was extracted with the RNeasy Micro kit (Qiagen). To construct library using the NEBNext Small RNA library prep kit (Illumina). Libraries were sequenced using the HiSeq 2500 system (Illumina).