Environmental foraminiferal 18S ribosomal DNA and cDNA multiplexed samples from New-Zealand fish farms sediments
For both DNA and RNA extractions performed on 48 sediment samples and cDNA synthesis for RNA, we PCR amplified the 37f hypervariable region of the foramnifera using tagged PCR primers prior to library preparation (TruSeq) and MiSeq paired-end sequencing. The combinations of tagged primers are contained in the paried reads and could be used to assign the sequences the their sample of origin.