GSM3603250: ATAC-Seq - NeuN positive/TDP-43 negative S1; Homo sapiens; ATAC-seq
Nuclei were extracted from frozen post-mortem frontal cortex and neuronal nuclei populations were obtained by FACS. ATAC-seq libraries were created as described in Buenrostro et al. 2015. Briefly, cells were lysed and treated with Tn5 Transposase from the Nextera Library Prep kit from Illumina for 30 min at 37°C. DNA was purified with Qiagen MinElute PCR purification kit and libraries were generated by PCR amplification using Nextera Index Primers from Illumina.
GEO Accession
GSM3603250