GSM4217311: Drd1-EGFP/Rpl10a (CP73) accumbens older mouse IP Rep1 [1614]; Mus musculus; RIP-Seq
The dorsal striatum and accumbens nucleus were dissected  and homogenized and tagged polysomes were immunopurified with monoclonal anti-EGFP antibodies bound to biotinylated-Protein L (Thermo Fisher) coated streptavidin-conjugated magnetic beads (Life Technologies). Total RNA from IPs was purified with the Agilent Nanoprep kit following manufacturer's protocol. RNA was extracted and treated as described in PMID 24810037, Heiman M, Kulicke R, Fenster RJ, Greengard P, Heintz N. (2014) Cell type-specific mRNA purification by translating ribosome affinity purification (TRAP). Nat Protoc. 9:1282-1291
GEO Accession
GSM4217311