GSM4417543: E17endo_1; Mus musculus; RNA-Seq
RNA from freshly FACS sorted cell populations, from dissociated fetal hearts,  was isolated using the Arcturus PicoPure RNA Isolation Kit (Applied Biosystems). Library preparation (New England Biolabs Nest Ultra RNA library prep Kit) and single read Next-generation sequencing (Illumina HiSeq 2500) were performed at the Genomics Unit at Centro Nacional de Investigaciones Cardiovasculares (CNIC). 200 ng of total RNA were used to generate barcoded RNA-seq libraries using the NEBNext Ultra RNA Library preparation kit (New England Biolabs). Briefly, poly A+ RNA was purified using poly-T oligo- attached magnetic beads followed by fragmentation and then first and second cDNA strand synthesis. Next, cDNA ends were repaired and adenylated. The NEBNext adaptor was then ligated, followed by uracile excision from the adaptor and PCR amplification. Finally, the size of the libraries was checked using the Agilent 2100 Bioanalyzer and their concentration was determined using the Qubit® fluorometer (ThermoFisher Scientific)
GEO Accession
GSM4417543