RNA-seq of Danio rerio: WT embryos at shield stage replicate 1
The RNA was extracted using Trizol according to the manufacturer s manual. Then the RNA was purified using RNA purification kit (Tiangen, China). The RNA samples were quantified and integrity was assessed by the Agilent 2100 Bioanalyser. The RNA integrity Numbers (RIN) of all RNA samples were >8.0. The RNA libraries were prepared using the Illumina TruSeq RNA sample preparation kit v2. The amount of input RNA is 1  g. The average final library size is 309 bp.