Illumina reads of chemosensory targeted sequence capture
Olfactory receptors identified from the transcriptome were used to design probes for an olfactory receptor targeted sequence capture. Pooling 3,814 chemosensory genes originally identified from the olfactory transcriptomes of twelve species of bats, probes were designed from RNA-Seq data to make 120-bp probes with 2X tiling density. The initial raw number of probes was 45,052, and given the duplicative nature of the genes, we clustered similar probes with 95% nucleotide identity of one another. The final probe count was 16,468 custom targets designed for chemosensory genes. Reads were first trimmed for quality using the same bbduk.sh script from the transcriptome assembly and exact duplicate reads were removed using ParDRe v. 2.2.5.