GSM2901785: zebrafish_resection_12_3; Danio rerio; RNA-Seq
Total RNA from the lower half of the ventricle of resected and sham hearts was isolated using Trizol at three different time points: 12, 24, and 48 hours post-surgery. Three biological replicates were used for each time point and condition in each species. For neonatal mice and axolotls, each replicate represents myocardium from a single animal. Due to the smaller size of the zebrafish, each biological replicate represents three pooled tissue samples. Poly-adenylated mRNA was isolated using the Wafergen #400047 protocol. RNA-sequencing libraries were prepared with the IntegenX Directional PrepX mRNA kit. Paired-end (50 base pairs) sequencing was performed using an Illumina HiSeq 2500.
GEO Accession
GSM2901785