GSM2898299: brm input - rep2; Arabidopsis thaliana; ChIP-Seq
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with an H2A.Z antibody described previously (Deal et al. (2007) Plant Cell 19(1):74-83). DNA collected from the IP and input material was purified using 1.8x volume of SPRI beads (Beckman Coulter). Libraries were prepared from 1 ng of DNA per sample with the Accel NGS 2S Plus DNA Library kit (Swift Biosciences) and sequenced on an Illumina NextSeq500 using 76 nt single-end reads.
GEO Accession
GSM2898299