GSM4120173: HeLa_mixTrail; Homo sapiens; RNA-Seq
Cell Hashing protocol with Hashtag oligonucleotide (HTO) was then conduct with 10x Genomics single-cell 3' V2 chemistry to generate single-cell emulsion. Single-cell RNA-seq was performed following manufacturer's protocol (Chromium™ Single Cell 3' Reagent Kit, V2 Chemistry) to obtain single cell 3' libraries for Illumina sequencing. PCR of 12 cycles was performed for cDNA amplification with HTO primer {Stoeckius:2018fd}. For HTO library preparation, 10 cycles of PCR amplification (REF Stoeckius M, 2018) followed by a size selection with Blue Pippin 3% agarose gel cassette (BDQ3010) Marker Q3 were necessary to select 180bp specific amplicon. Final cDNA library was then pooled with 10% of HTO library in final pool for sequencing.
GEO Accession
GSM4120173