All WGS libraries for E. flavifrons sample Harlow, the source of the reference genome
PE libraries of target insert sizes 180 bp, 500 bp, and 1 kb were prepared using the recommended Illumina protocols at the University of Chicago and sequenced using an Illumina HiSeq2000 at Princeton University (Princeton, NJ). PE libraries with mean target insert size of 1 kb were prepared with Illumina’s TruSeq DNaseq Sample prep kit and MP libraries were prepared with Illumina’s Mate-pair version 2 Library prep kit at the Keck Sequencing Center of the University of Illinois (Champaign, IL); these libraries were also sequenced at the Keck Center. PE libraries were quantitated by qPCR and sequenced for 100 cycles from each end of the fragments on a HiSeq2000 machine using a TruSeq SBS sequencing kit version 3. Reads were analyzed with Casava 1.8 (pipeline 1.9) and subsequently stripped of adaptors.