GSM4120180: Bioprinted kidney organoid; Homo sapiens; RNA-Seq
CRL1502.C32 kidney organoid were dissociated at D7+18 using cold active protease (Liberase) for 20 minutes at 4c. Cells were centrifuged at 1500 rpm for 3minutes to form a pellet. Single cells were resuspended in fresh DMEM F12 media filtered through 20 µm cell strainers to remove clumps, and stored on ice until analysis. Chromium Single Cell Library kit V2; Illumina HiSeq HT chemistry- 100bp paired end sequencing
GEO Accession
GSM4120180