Sequencing of KO13PolIB mutant of Arabidopsis thaliana.
DNA was isolated from ˜400 mg pools of 14-day-old Arabidopsis plants using the cetyl trimethyl-ammonium DNA extraction protocol. DNA was fragmented to ˜200-500 bp using S-Series Covaris according to Illumina’s specifications. Libraries were prepared using the TruSeq DNA library preparation kit (Illumina) according to manufacturer’s instructions. Efficient library generation was then assessed using a Bioanalyzer platform (Agilent) and Illumina MiSeq-QC run was performed. Sequencing was performed using an Illumina HiSeq2000 using TruSeq SBS v3 chemistry at the Institute for Research in Immunology and Cancer’s Genomics Platform (Université de Montréal). Cluster density was targeted at around 600 to 800 kilo clusters mm–2.