Willow transcript sequencing
Willow transcript sequencing
Total RNA was isolated using CTAB-LiCl method (Chang et al. 1993). The integrity of total RNA was determined by gel electrophoresis, and RNA concentration was measured using a Nanodrop-2000 spectrophotometer (Thermo, inc). Then, DNA residues was digested by DNase (TaKaRa, inc) at 37 C for 30 minutes. Subsequently, the extracted RNA was purified using Oligotex mRNA Mini Kit (Qiagen, inc). At last, size and concentration of mRNA were quantified using a RNA 6000 Pico Chip on the Agilent 2100 Bioanalyzer (Agilent, inc).
Experimental Factor: sex
male