Puccinia striiformis f.sp. tritici UK race 08/21 shotgun sequencing
Genomic DNA was extracted for each race from dried urediniospores using the CTAB method.  A single DNA library was prepared from 5 *g of total genomic DNA that was randomly fragmented using the Bioruptor sonicator (Diagenode). The library was prepared with the NEB Next DNA Sample Prep Master Mix according to manufacturer's instructions (New England Biolabs, MA USA).  Fragments were purified using the Nucleotrap kit (Clontech) and eluted in 30 ul elution buffer and PCR-enriched using 14 PCR cycles. Paired end adapters and PCR primers were obtained from Illumina. Primers with 6 nucleotide long barcodes were used. Library quality and quantity were confirmed before sequencing using a Bioanalyzer (Agilent).