RNA-Seq of treeshrewadult male PBMC
To remove ribosomal RNA, 1 ug RNA was fed into the Ribo-Zero Magnetic Gold         Kit (Illumina, catalog #MRZG12324) followed by a cleanup using Agencourt RNAClean XP beads         (Beckman Coulter,catalog #A63987), elution with 5 L RNA Fragmentation and Priming mixed from         the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, catalog #E7420L).         Library construction was performed per the vendors instructions, starting with the RNA         Fragmentation, Priming and First Strand cDNA Synthesis step. Library quality was evaluated         on the Agilent 2100 bioanalyzer and quantified by qPCR using the KAPA Library Quantification         Kit-Illumina/Universal (KAPA Biosystems, catalog #KK4824).