GSM1612779: miCLIP_NSUN3_HEK293_rep1; Homo sapiens; RIP-Seq
For miCLIP, myc-tagged NSUN3 or NSun6 constructs were transfected into HEK293 cells which were harvested 24 hours post-transfection. Cells were then lysed in lysis buffer and treated with high concentration of DNase and low concentration of RNaseI to partially fragment RNAs. Lysates were incubated with proteinG dynabeads in the presence of anti-myc antibody to isolate NSUN-RNA complexes.  Libraries were then prepared following isolation of RNAs from the protein-RNA complexes. Libraries were prepared using Illumina TruSeq RNA prep kits
GEO Accession
GSM1612779