GSM3683934: 5637 NR control-2 [272 DS-8]; Homo sapiens; RNA-Seq
RNA was extracted using ReliaPrep RNA Cell Miniprep system (Promega). Total RNA was quantified using the Quant-iT Ribo-Green RNA Assay Kit (Invitrogen). Automated Illumina  Tru Seq Sample Preparation protocol was used to separate mRNA from total RNA, followed by cDNA  18  synthesis. Libraries were prepared using TruSeq® Stranded mRNA Library Prep kit (Illumina). Following  quality check, the libraries were quantified using the KAPA Library Quantification Kits for Next-Generation  Sequencing (Kapa biosystems), pooled and sequenced on the HiSEq3000 platform targeting 50 million  paired end reads/sample.
GEO Accession
GSM3683934