GSM2476296: ngr1_KO_crym3; Mus musculus; RNA-Seq
GFP+ Corticospinal motor neurons from crym-GFP transgenic wild type and ngr1-/- mice were harvested via laser capture microdissection from flash frozen brains, cut on a cryostat. RNA was harvested with QIAGEN Rneasy Micro kit (cat#74004). 500pg of total RNA was processed with Clontech's SMARTer Ultra Low Kit (cat# 634888) was used for the construction of sequencing libraries. Covaris AFA system was used prior to standard Illumina protocols.
GEO Accession
GSM2476296