WGS of Bacillus thuringiensis: VBT62 strain
Whole genome sequencing of Bacillus thuringiensis str. VBT62 was done. For this DNA was normalized to 1000 ng in volume of 130  l, the samples were then subjected to mechanical shearing using microTUBE AFA Fiber SnapCap tubes on M220 Focused-ultrasonicator. The DNA was targeted for the fragments in the range of 250 bp to 300 bp using Ampure purification. The fragmented DNA molecules were taken for whole genome DNA library preparation using NEBNext  Ultra  II DNA Library Prep kit (E7645L) by following the standard protocol. The final library was validated using Agilent DNA chip on Agilent 2100 Bioanalyzer system. Further, the library was quantified using QUBIT dS DNA HS Kit. . The final library was loaded on to NextSeq 500 reagent cartridge for the cluster generation and sequencing.