GSM4670648: EK084: Human sigmoid mucosa - Lean patient; Homo sapiens; RNA-Seq
Sigmoid mucosal biopsies were cryopreserved and FACS isoleted, using a BD FACSAria II cell sorter. A QC analysis was completed using a Vi-Cell XR to evaluate quality and compatibility of cells. Reverse transcription was conducted using Chromium Single Cell 3′ Library & Gel Bead Kit v2 (10X Genomics). cDNA was recovered. Pre-amplified cDNA was further subjected to library preparation. Libraries were sequenced on an Illumina Hiseq 4000
GEO Accession
GSM4670648