Whole genome PacBio sequencing of Plasmodium knowlesi Malayan Strain Pk1 (A+)
Genomic DNA was extracted from ex vivo matured schizont-stage parasites with a Qiagen DNA blood midi kit. The gDNA was further purified with a PowerClean DNA Clean-up kit (Mo Bio Laboratories). Five micrograms of gDNA were subsequently used for library preparation. SMRTbell® DNA libraries were constructed according to the PacBio standard protocol with BluePippin® Size-selection system. Sequencing was performed on a PacBio RSII® instrument using P6-C4 chemistry.