GSM2563159: Si_WL_rep_1; Setaria italica; DNase-Hypersensitivity
Small fragments of DNA (~50-600bp) were isolated using gel purification following phenol chloroform extraction of DNaseI treated nuclei. Libraries were created using the Hyper Prep DNA Library preparation kit  according to the manufactures instructions and indexed for pooling using NextFlex DNA barcoded adapters (Bioo Scientific, Austin TX US). Libraries were quantified on a Tapestation DNA 1000 Screen tape and by qPCR using an NGS Library Quantification Kit (KAPA Biosystems) on an AriaMx qPCR system (Agilent). Libraries were then normalised, pooled, diluted and denatured for sequencing on the NextSeq 500 (Illumina, Chesterford UK) according to the manufacturer’s instructions. PhiX control library (Illumina) was spiked into the main library pool at 10% for quality control purposes and to improve library diversity.
GEO Accession
GSM2563159