GSM3901290: TC-797_DMSO_replicate2 (TT-seq); Homo sapiens; RNA-Seq
Cells were lysed with TRIzol (Thermo Fisher #15596018) and total RNA was isolated essentially following the manufacturer's instructions. Based on the yield of total RNA  the amount of total RNA per 10 million cells was determined and 400 ng of 4-thiouridine labelled total Drosophila Kc167 cell RNA was added/spiked-in to an aliquot of total RNA corresponding to 10 million TC-797 cells. RNA was fragmented using a NEBNext RNA Fragmentation Module and then labelled with MTSEA-biotin-XX. Labelled RNA was enriched using streptavidin Dynabeads. rRNA was depleted from the sample using a NEBNext rRNA Depletion Kit (New England Biolabs #E6310). A DNA library (from reverse transcribed RNA) was prepared for high-throughput sequencing following chapter 5 in the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs #E7760).
GEO Accession
GSM3901290