Comparative transcriptomic analysis of male and female flower buds of Idesia polycarpa Maxim.
Total RNA of female flower buds of I. polycarpa was extracted using the TRIzol Reagent (Invitrogen, USA). A total amount of 3 μg RNA was used as input material for the RNA sample preparations and was fragmented. Then the fragments were used to synthesize the first-strand cDNA.Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H. The short double-stranded cDNA fragments were connected with sequencing adapters for PCR amplification as templates. Subsequently, the female library was sequenced on an Illumina Hiseq platform and paired-end reads were generated.