RNA-Seq of root: adult Bletilla
The nuclear ribosomal internal transcribed spacer (ITS) region was amplified with the fungal-specific primers ITS1F and ITS4 (Liebel et al., 2015). All positive PCR products were purified with the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) and sequenced bidirectionally on Illumina MiSeq PE300 platform (Illumina, San Diego, CA, USA) according to the standard protocols from Majorbio Bio-Pharm Technology Co. Ltd. (Shanghai, China).