Targeted-Capture of Homo sapien: Adult Male: Sample 905
Genomic DNA was prepared from venous blood samples with the PAXgene Blood DNA kit (Qiagen). DNA was sheared (Covaris) and ligated to adapters necessary for both sequencing and barcoding samples using the TruSeq kit (Illumina). The OR subgenome was captured using an Agilent SureSelect Target Enrichment kit custom-designed to enrich for the open reading frame of ORs and human orthologs of 256 additional genes expressed in mouse olfactory sensory neurons (SureSelect ELID 0352781). Paired-end sequencing was carried out on 332 participants using an Illumina GAIIx with a read length of 2x75 basepairs. Each sample was individually barcoded and twelve samples were multiplexed per lane.
alignment_software
Burrow-Wheeler Aligner (0.7.10-r789)