GSM1614177: 3' end sequencing for HeLa cells; Homo sapiens; RNA-Seq
Trizol (Invitrogen) and Dynabeads mRNA Purification Kit for mRNA Purification (Invitrogen) The sequencing library was constructed from 400ng poly(A) RNA using NEBNext mRNA Library Prep Reagent Set for Illumina (New England Biolabs) following manufacturer's instructions with a custom primer for reverse transcription and custom adaptors for ligation (both are adapted from (Jenal et al. 2012)).
GEO Accession
GSM1614177