Primary mouse hepatocytes, Input control ChIP-seq
Input represents a pool of chromatin from 4 different treatments (Control, Dexamethasone, GW7647, Dexamethasone/GW7647). Sample from each treatment was obtained by pooling 2 biological replicates. Each biological replicated was obtained by pooling hepatocytes from 3 mice.    Cell pellets were lysed with 500 μl of lysis buffer (0.1% SDS, 1% Triton X-100, 0.15M NaCl, 1mM EDTA, 20mM Tris pH 8) supplemented with protease inhibitor cocktail (Pierce, cat. 88265) and sonicated 30x (30s on/ 30s off, high intensity) using a Bioruptor sonicator (Diagenode) at 4°C. Insoluble material was removed by centrifugation (30 min, 20000g). 10μl of each input chromatin sample was diluted to 200μl with elution buffer (1% SDS + 0.1M NaHCO3) and cross-links were reversed at 65°C overnight by adding 8μl of 5M NaCl, together with 8μl of RNAse A (0.5 mg/ml, Macherey-Nagel, cat. 740505) and 10 μl of Proteinase K (20 mg/ml, Qiagen, cat. 19131). DNA was purified with a QIAQuick PCR purification kit (Qiagen, cat. 28106) according to the user’s manual.