RNA-Seq of Magnolia denudata: non-thermogenic flowers
Approximately 10 ug of total RNA representing a speci c adipose type was sub-jected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Thermo- sher). Following puri cation,the mRNA is fragmented into small pieces using divalent cations under levated temperature. Then the cleaved RNA fragment were constructed into the  nal cDNA library in accordance with the protocol for the Illumina RNA ligation based method (Illumina, San Diego, USA).