GSM1931268: Hippocampus_High-CPF_mRNA1; Rattus norvegicus; RNA-Seq
Total RNA including small non-coding RNA was isolated from the CA1 region of rat hippocampus using miRNeasy kit (Qiagen, Valencia, CA) according to the manufacturer’s protocol. Thirteen rat hippocampus RNA samples were selected from a CPF treatment group (10 mg/kg b.w./day CPF, n=6) or a vehicle control group (n=7). Total RNAs were processed for RNA-seq using the TruSeq RNA sample prep kit v2 (Illumina, San Diego, CA) according to the manufacturer’s protocol (Illumina, San Diego, CA). The rat hippocampus RNA libraries were clustered onto a TruSeq V3 flowcell, and subjected to a 100-cycle single read run (1x100 cycles) on Illumina HiScanSQ sequencing instrument according to the protocols described by the manufacturer (Illumina, San Diego, CA).
GEO Accession
GSM1931268