WGS of Natronobacterium gregoryi SP2
The genomic DNA fragments of N.gregoryi SP2 were extracted and then separated by agarose gel electrophoresis with 1% gel. The TruSeq DNA Library Preparation Kits were used to prepare DNA libraries with insert sizes from 400-500 bp for paired-end sequencing. Blunt-end DNA fragments were generated using a combination of fill-in reactions and exonuclease activity. An 'A'-base was then added to the blunt ends of each strand, preparing them for ligation to the sequencing adapters. Each adapter contains a 'T'-base overhang on the 3'-end, providing a complementary overhang for ligating the adapter to the A-tailed fragmented DNA. These adapters contain the full complement of sequencing primer hybridization sites for paired-end reads. Following the denaturation and amplification steps, libraries can be pooled for sequencing.