Paired Illumina Whole Exome Sequencing of Burkitt Lymphoma study sample N670
Up to 3 ??g of genomic DNA was sheared to a target size of 250 bp by Covaris adaptive-focused acoustics with duty cycle of 10%, intensity of 5, frequency of 200 cycles/burst, duration of 135 s and water bath temperature of 4 ??C. Purified sheared DNA was end repaired, A tailed, ligated to Illumina paired-end adapters and amplified as described in the Agilent SureSelect Target Enrichment System for Illumina Paired-End Sequencing Library. The ligated paired-end library was PCR amplified using Illumina paired-end PCR primers and 2?? Phusion High-Fidelity Master Mix (NEB). The library was column purified and assayed on a Bioanalyzer to determine size and concentration.