GSM2396978: C2-PBmPA-Day5_3; Homo sapiens; RNA-Seq
miRNeasy mini kit (Qiagen) + DNase treatment KAPA Stranded RNA-Seq Kit. The workflow consists of mRNA enrichment, cDNA generation, and end repair to generate blunt ends, A-tailing, adaptor ligation and PCR amplification. Different adaptors were used for multiplexing samples in one lane. Sequencing was performed on Illumina HiSeq2000 for a single read 50 run. Data quality check was done on Illumina SAV. Demultiplexing was performed with Illumina Bcl2fastq2 v 2.17 program.
GEO Accession
GSM2396978