GSM1695574: MLM-P9A; Mus musculus; miRNA-Seq
To obtain the lenses, mice were first anesthetized by isoflurane and subsequently euthanized through cervical dislocation. The lenses were isolated using forceps under a microscope. Two biological replicates, each consisting of 8-9 pups, were used for each developmental stage. Lenses were dissolved immediately in the Trizol reagent (Invitrogen; Carlsbad, CA) and total RNA was extracted. Illumina TruSeq RNA Sample Preparation Kit v2 and TruSeq Small RNA Sample Prep Kit was used to prepare mRNA and small RNA sequencing libraries
GEO Accession
GSM1695574