Metagenomics analysis of Plutella xylostella pupal gut microbial symbionts
DNA library construction was performed following the manufacturer’s instruction (Illumina). We constructed one paired-end (PE) library with insert size of 350 bp for each sample. Then high-throughput sequencing was performed using Illumina Hiseq 2000 to obtain reads data for all samples. The reads length for each PE end is 100 bp.