miRNAs of Rimicaris exoculata
Qualified RNAs were size-fractionated to isolate small RNAs, which were then ligated sequentially to 3’ and 5’ adapters. Reverse transcription and PCR amplification were performed after ligation. The resulting products were once again size-fractionated to purify cDNA. The purified cDNA was used for cluster generation on Illumina’s Cluster Station and sequenced using Illumina GAIIx platform.