MiSeq reads for Phytophthora nicotianae race 1
For MiSeq sequencing, genomic DNA of P. nicotianae was first fragmented at 500 bp and tagged with sequencing adapters using TrueSeq DNA Sample Prep Kit, then amplified using limited-cycle PCR. PCR library DNA was then purified using Agencourt AMPure XP beads (Beckman Coulter, Item # A63882), which excluded very short library fragments. DNA libraries were then quantified using the Qubit assay and qualified using the Agilent Technologies High Sensitivity DNA Kit (Item # 5067–4626). Purified libraries were pooled and sequenced on the MiSeq using a 2×300 paired end protocol. Initial basecalls were converted to fastq files using MiSeq CASAVA software suite.