CFIAFB20190004_Listeria_monocytogenes
Brain Heart  Infusion broth was inoculated with a single colony of bacteria and incubated  for 4 to 6 h at 35C. Genomic DNA was isolated from 400 L of culture using  the Maxwell 16 Cell SEV DNA Purification kit (Promega, Madison, WI). DNA was  then quantified using the Quant-iT High-Sensitivity DNA Assay Kit (Life  Technologies Inc., Burlington, ON). Sequencing libraries were constructed  from 1 ng of gDNA using Nextera XT DNA Sample Preparation and Index Kits  (Illumina, Inc., San Diego, CA) according to manufacturers instructions.  Genomic sequencing was performed on the Illumina MiSeq Platform (Illumina,  Inc.) using a 600-cycle MiSeq Reagent kit v3 (Illumina, Inc.).