NextSeq 500 sequencing; RNA-seq in HUVECs overexpressing Sox18 and treated with Sm4
RNA-seq in HUVECs overexpressing Sox18 and treated with Sm4
HUVECs were obtained from LONZA and grown according to suppliers recommendations. Cells were cultured in EBM2 media, supplemented with EGM2.  Transfection (7 hours) was performed using Roche X-tremegene DNA transfection reagent in 0.4% FBS media.  Directly following transfection, cells were stimulated (14 hours) with small molecule (or DMSO), in 0.4% FBS media. RNA was extracted using RNeasy mini kit (Qiagen). Samples (>100 ng RNA) were processed for whole transcriptome sequencing using TruSeq stranded total RNA library prep kit (Ribo‐Zero GOLD, Illumina).
Experimental Factor: none
compound
Experimental Factor: transfected with Sox18-cMyc
genotype