GSM3683705: 9747279_B: 1169; Homo sapiens; RNA-Seq
The buffy coats stored in -80ºC were thawed on ice for two hours and transferred into PAXgene tubes (PreAnalytiX), and the RNA was extracted using the PAXgene RNA extraction kit. ~500ng total RNA per sample was used for library prep using the TruSeq RNA Access Library Prep Kit (Illumina, Inc). Libraries were then quantified and normalized to 2nM prior to sequencing on a NextSeq 500 sequencer using NextSeq 500 High Output Kit -150 cycles (Illumina, Inc), generating about 60 million paired-end reads for each sample.
GEO Accession
GSM3683705