AireKO medullary epithelial cells RRBS replicate 2
Reduced Representation Bisulfite Sequencing (RRBS) libraries were prepared as described 50 from 5-10 × 104 sorted MECs (one mouse) except that EZ DNA Methylation-Direct (Zymo) was used for bisulfite conversion. Sequencing (single-end, 50bp) was performed on a HiSeq2000. Prior to alignment, reads were trimmed to remove adapter sequences using the RRBS option in TrimGalore! (Babraham). Trimmed reads were aligned to mm10 using Bismark 57 (Babraham).