GSM3004752: oTC2R46; Mus musculus; RNA-Seq
Tumors from young and aged mice were homogenized in Qiagen RLT buffer, and RNA was isolated using the RNeasy extraction kit (Qiagen, Valencia, CA). RNA concentration, purity, and integrity were assessed by NanoDrop (Thermo Fisher Scientific Inc) and Agilent Bioanalyzer. RNA-seq libraries were constructed from 1 ug total RNA using the Illumina TruSeq mRNA LT Sample Prep Kit according to the manufacturer's protocol. Barcoded libraries were pooled and sequenced on the Illumina HiSeq 2500 generating 50 bp paired end reads.
GEO Accession
GSM3004752