DNA-Seq of Staphylococcus aureus USA 100:  Illuina NextSeq
Genomic DNA was prepared for shotgun metagenome sequencing using the Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA). Briefly 1ng of gDNA was prepared according to manufacturers instructions. The resulting library was sequenced on an Illumina NextSeq500 instrument, employing paired-end 150 base reads.