RNA-seq of Rat Cardiomyoctyes:  Adult Heart
5ug RNA per sample was processed using poly-A selection and fragmentation.  First strand cDNA was generated .  Adapters were ligated using the Illumina Paired-End Sample prep kit.  Fragments of ~200 bp were isolated using gel electrophoresis, amplified by 15 cycles of PCR and sequenced on the Illumina Genome Analyzer II