GSM2898203: Ma-IP-replicate2; Mus musculus; RIP-Seq
Total RNA of BMDCs was isolated with Trizol regent (Invitrogen) and mRNA was enriched by Dynabeads mRNA Purification Kit (Invitrogen). Removal of ribosomal RNA was confirmed by 2200 Tape Station detection (Agilent). About 8μg mRNA was fragmented to 100-200bp in length and 1/10 volume was separated as input. The remaining fragmented RNA was mixed with dynabeads protein A (Life technology) pre-mixed with anti-m6A antibody overnight. The library preparation was constructed according toNEBNext Ultra RNA Library Prep Kit for Illumina
GEO Accession
GSM2898203