GSM4120749: D_CLIP_PC2_3_S21_R1_001; Drosophila melanogaster; RIP-Seq
trizol extraction of RNA from wildtype flies followed by formaldehyde crosslinking to recombinant drosophila melanogaster CMTR1 or CMTR2, protein-bound RNA was proteinase K treated and Trizol-extracted. Lexogen's quant-seq 3'FWD kit was used, using a random nonamer tagged partial p7 sequence (i.e. CACGACGCTCTTCCGATCTNNNNNNNNN) in place of the partial p7 sequence containing anchored oligoDT primer found in the manufacturer's protocol. The remainder of the protocol was performed as prescribed by Lexogen (Vienna). Size selection of libraries were carried out with PAGE.
GEO Accession
GSM4120749