GSM1930823: Single macrophage #16; Mus musculus; RNA-Seq
Wildtype macrophages were flowed into a C1 IFC for mRNA seq (10-17 μm) chip using the Fluigidm system. Lysis, reverse, transcription and PCR were performed using the SMARTer Kit designed for the C1 (Clontech, Mountain View, CA). The efficiency of chip loading (capture) was confirmed by microscopy and any of the wells that contained either none or more than one cell were noted and discarded from further library preparation and analysis. 24 wildtype single cDNA libraries were selected for library preparation on the basis of concentration and size range, as determined via Agilent Bioanalyzer. Sequencing libraries were made from the cDNA using the Nextera XT DNA Sample Preparation Kit (Illumina).
GEO Accession
GSM1930823