RNA-Seq of Sus  scrofa: juvenile female blood
Total RNA from  sorted TCR-gd CD2- cells of a four-month-old conventional pig was  reverse-transcribed into cDNA using the SMART technology and oligo(dT)  primers (SMARTer PCR cDNA synthesis kit). Following reverse transcription,  the cDNA population was enriched for full-length V(D)J TCR transcripts with  pTRDC primers including Illumina adaptor sequences as overhangs for Illumina  MiSeq analysis.