Illumina HiSeq 2000 paired end sequencing; Arabidopsis RNA-Seq Differential Gene Expression profiling
Arabidopsis RNA-Seq Differential Gene Expression profiling
Seedlings were harvested after 14 days. WT A. thaliana Col-0 seeds were sown aseptically on MS10 plates, stratified for 2 days at 4 degrees C and then grown at a constant 21 degrees C under a 16-h light/8-h dark cycle for a further 14 days. Total RNA extraction with RNeasy Plant Mini Kit (Qiagen) then treated with TURBOTM DNase (Ambion). 4 μl of ERCC spike-ins at a 1:100 dilution were added to 1 μg/6 μl of total RNA. Illumina TruSeq Stranded Total RNA with Ribo-Zero Plant.
Experimental Factor: 3
individual