GSM2059232: Cre-Cntrl-rep5; Mus musculus; RNA-Seq
Cardiomyocyte nuclei were isolated as previously described (Preißl et al. 2015, Circ Res.). Nuclear RNA was extracted using the RNeasy Micro Kit (Qiagen). RNA was reverse transcribed and amplified with the Nugen Ovation RNA-seq System V2 (Nugen). The resulting cDNA was fragmented using a Bioruptor (25x 30/90 seconds, low, Diagenode). Sequencing libraries were constructed using the NEBNext Ultra DNA Library Prep Kit (NEB) from 100ng fragmented cDNA with 9-13 PCR cycles.
GEO Accession
GSM2059232