RNA-Seq of human: adult duodenum
Total RNA was  extracted from tissue samples using MagMAX kit (Life tech, Carlsbad, CA).  mRNA was purified from 4 ug total RNA using Dynabeads mRNA Purification Kit  (Invitrogen, Waltham, MA). Strand-specific RNA-seq libraries were prepared  from 500 ng RNA using KAPA stranded mRNA-Seq Kit (KAPA Biosystems). The  libraries were amplified by twelve-cycle PCR. Sequencing was performed on  Illumina HiSeq2000 (Illumina) by multiplexed paired-read run with 100  cycles.