GSM1613091: Hdac1_ChIP; Mus musculus; ChIP-Seq
Cells were cross-linked, lysed and sonicated to generate DNA fragments with an average size of 200 bp 10-15 ng IP DNA and input DNA measured by Qubit Fluorometer (Invitrogen) were used to construct DNA library by using ChIP-Seq Sample Prep Kit (Illumina) according to Illumina's instructions. Adapter-modified DNA between 200 and 350 bp were isolated by agarose gel electrophoresis and purified. Then, DNA was enriched by 18 cycles of linker-mediated-PCR using primers from Illumina.
GEO Accession
GSM1613091