RNAseq of SmUW234 in PHBneg media Rep1
Ribosomal RNA depletion was performed on 1 µg of total RNA with the Meta-Bacteria Ribo-Zero rRNA Removal Kit (Mandell Scientific, Guelph, Canada), at the Genome Quebec Innovation Center of McGill University. Final RNA was isolated using the RiboMinus Concentration Module and eluted using 17 µl of “Fragment, Prime, Finish” buffer. (Life Technologies). The eluted RNA was then fragmented and prepared for cDNA synthesis. The TruSeq Stranded mRNA Sample Prep Kit was used for cDNA synthesis and then quality of the library and fragment size was assessed using a Labchip and Light Cycler 480 II and an Infinite M200 Fluorimeter (Roche, Mississauga, Canada and Tecan, Mannedorf, Switzerland).