GSM3736911: 10_P_4; Canis lupus familiaris; RNA-Seq
Following surgical ovariectomy, corpora lutea were trimmed from surrounding tissue. Rinsed with phosphate-buffered saline (PBS) solution and immersed in RNA®later for 24h at 4oC. After 24h, tissue material was divided and stored in -80oC until further usage. RNA was isolated using Trizol reagent, following the manufacturer's recommendations. Illumina TruSeq RNA Sample Prep Kit v2 was used with 100-1000ng/per sample of total RNA for the construction of sequencing libraries. RNA-seq polyA-selection
GEO Accession
GSM3736911