GSM2398479: GcgR KO rep3; Mus musculus; RNA-Seq
Total RNA was isolated with Trizol (Invitrogen, 15596-018) by a chloroform extraction, and was precipitated by isopropanol and redissolved in 20 µL of RNase free water. After digesting the sample with TURBOTM DNase (Ambion, AM2239), we extracted RNA (in 100 µL water) with chloroform/phenol and precipitated RNA with 250 µL of 100% ethanol and 10 µL of 3M NaOAc at -80 °C overnight. Ribosome RNA was removed by using Ribo-ZeroTM rRNA removal kit (Epicenter, MRZH116). The purified RNA was used for the cDNA library preparation using the TruSeq Stranded mRNA Library Prep Kit. The library was sequenced using the Illumina HiSeq 2000 platform through 50-cycle single-end runs.
GEO Accession
GSM2398479