GSM3003478: ATACseq_control_2_p8; Homo sapiens; ATAC-seq
Cells were lysed with ice-cold hypotonic cell lysis buffer (0.1% w/v Sodium Citrate Tribasic Dihydrate and 0.1% v/v Triton X-100) for 30 min on ice followed by a second 30 min lysis step with ice-cold cell lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl 2, 0.1% v/v IGEPAL CA-630). Transposition reaction was carried out in 25 μl reaction volume using 5 μl of tagmentation DNA enzyme in 2x TD buffer (FC-121-1030, Illumina). EDTA (25mM) was added to the reaction mix before DNA recovery with MiniElute PCR Purification columns (28004, Qiagen). ATAC-seq libraries were generated from 100,000 cells, as previously described (Buenrostro et al., 2013)
GEO Accession
GSM3003478