GSM2059379: SHM_Ubc_Duvelisib rep3; Mus musculus; OTHER
Cells were lysed in lysis buffer (NaCl 200 mM, Tris.HCl 100 mM, EDTA 5mM, SDS 0.2%)  containing 10 μg/ml Proteinase K  at 56°C overnight. Genomic DNA was precipitated with isopropanol at room temperature, washed in ethanol 70% and resuspended in TE buffer (10mM Tris-HCl plus 1mM EDTA) Phusion High Fidelity DNA polymerase was used to amplify selected regions from template genomic DNA. Amplification products were purified using PCR purification kit and GEL extraction kit and sequenced bi-directionally in a Mi-Seq Illumina sequencing platform
GEO Accession
GSM2059379