rpoB amplicon sequencing of freshwater sediment: Kevojarvi 2.4 cm
The PCR was performed using the EconoTaq PLUS MM Lucigen, Middleton, WI, USA). Temperature cycling consisted of: 94 C, 2 min; 35 cycles of {94 C, 30 s; 53 C, 30 s; 72 C, 30 s} , with a final elongation at 72 C for 5 min. All sequencing was performed on the Illumina MiSeq platform (paired-end 250 bp) according to the manufacturer's guidelines, at Gnome Qubec (Montreal, QC, Canada).