GSM2802906: HeLa mRNA 8cycle OED; Homo sapiens; RNA-Seq
Total RNA was harvested using Trizol reagent followed by iso-propanol precipitation. mRNA was then purified by the standard Aldrich mRNA extraction protocol. mRNA was fragmented by alkaline hydrolysis and  then eight rounds of oxidation/elimination/dephosphorylation (OED) were conducted, followed by 5?-phosphorylation and the last oxidation/elimination (OE) to block the fragments without Nm at the 3'-end. RNA libraries were then prepared for sequencing using standard NEB small RNA library protocols except using the new 3'- and 5'-adaptors.
GEO Accession
GSM2802906