ATM sequences from Hek293 cells treated with canonical TALENs
Sequencing from Hubbard et al, 2015.  293 cells were transfected with a plasmid expressing homodimeric FokI fusions to the canonical WT ATM-L TALE together with a plasmid encoding the canonical ATM-R TALE–FokI fusion protein. After 48 h, genomic DNA was harvested and the ATM target site and its corresponding known off-target sites OffA11 and OffA17 were amplified from this genomic DNA.  The amplified sites were then high-throughput sequenced.  All genomic target sites (ATM on-target, OffA11 and OffA17) were combined together for this one type of treatment with WT TALENs.