WGS of Pyropia yezoensis: Gametophyte
After removing surface bacteria through Physical vibration with quartz sands, Pyropia gametophytes were used to isolate genomic DNA. Illumina paired end sequencing libraries (500-bp insert sizes) and mate pair libraries (5 kb in size) were prepared according to the manufacturers instructions. Meanwhile, a total of 10 g of DNA was used toconstruct a 20-kb library using the PacBio Pacific Biosciences SMRT Bell Template Kit 1.0.