GSM1931800: NA12883cell; Homo sapiens; ncRNA-Seq
Small RNA from Trizol-lysed LCLs and exosomes was isolated from 1 µg total RNA using the TruSeq Small RNA Sample Prep Kit (Illumina, San Diego) with minor modifications. Small RNA libraries were constructed following standard Illumina protocols. PCR-amplified small RNA libraries were gel purified with 3% NuSieve GTG low-melt agarose (Lonza, Rockland) and target bands of 147–157 nt, containing adapter sequence of 125 nt, were excised and column purified (Qiagen, Germantown).
GEO Accession
GSM1931800