Dna-seq of homo sapien stool
The 16S rDNA V4 region was amplified by PCR and sequenced in the MiSeq platform (Illumina) using the 2x250 bp paired-end protocol yielding pair-end reads that overlap almost completely. The primers used for amplification contain adapters for MiSeq sequencing and single-index barcodes sothat the PCR products may be pooled and sequenced directly(3), targeting at least 10,000 reads per sample.