The test blood DNA sample enriched by Agilent SureSelect 50M and run on one lane of Illumina HiSeq 2000.
Libraries were denatured with sodium hydroxide and loaded onto an Illumina cBot for cluster generation according to the manufacturer's recommended protocols (TruSeq PE Cluster Kit v2, Illumina Cat# PE-401-2001). Lane 5 of each flowcell was reserved for a PhiX control (Illumina Cat# FC-110-3001). The primer-hybridized flowcells were then transferred to HiSeq 2000 sequencers and paired-end sequencing was performed with TruSeq SBS kits (Illumina Cat#FC-401-1001) in a 2 x 101b mode.