HaCaT-p63_AF1916-ChIPseq-1
50 million HaCaT cells were crosslinked with formaldehyde (1.5%) for 10 minutes at room temperature, collected by scraping, and sonicated to yield ~300 bp DNA fragments using a Diagenode Bioruptor. ChIP was performed using 10 g of p63 (AF1916, R&D Systems) antibody. Input control samples were collected from sonicated samples prior to performing ChIP. ChIP-seq libraries were prepared as described previously in Marshall et al., Cell Reports, 2016. Illumina sequencing of ChIP-seq libraries was performed at the Vanderbilt VANTAGE core using a single-end 50 bp protocol.