OP swab452 from a carriage study at a university
DNA was extracted from 200 L of STGG medium using QIAamp DNA Microbiome Kit (Qiagen) as per manufacturer protocol. DNA concentrations were measured using a Qubit 2.0 fluorometer with dsDNA High Sensitivity reagents (Invitrogen, Grand Island, NY, USA). The eluted DNA concentration ranged from 0.2 to 5 ng/L. Sequencing libraries were prepared from 2 ng of DNA using Nextera XT DNA library preparation kit following the manufacturers protocol (Illumina, San Diego, CA, USA). The resulting libraries were sequenced Illumina HiSeq 2500 or MiSeq to obtain 250 bp paired-ends reads.