GSM1613682: Ago2-HITS-CLIP, biological rep.4; Mus musculus; RIP-Seq
Keratinocytes were lysed in Lysis Buffer (50mM Tris pH 7.4 100mM NaCl 1mM MgCl2 1% NP-40 .5% Na-Deoxycholate .1% SDS). Ago2-RNA crosslinked material was recovered using IP, and purified via Novex PAGE followed by transfer to nitrocellulose and extraction with Phenol-Chloroform Ago2-Hits-Clip libraries were prepared using methods described in Wang, Zhen, James Tollervey, Michael Briese, Daniel Turner, and Jernej Ule. (2009) CLIP: Construction of cDNA Libraries for High-Throughput Sequencing from RNAs Cross-Linked to Proteins in Vivo. Methods 48, no. 3 (July 2009): 287–93.
GEO Accession
GSM1613682