GSM1611999: Sample_77-L; Sus scrofa domesticus; RNA-Seq
Total RNA was isolated and purified from LM and adipose tissues using Ambion MagMAX total RNA isolation kits (Life Technologies, Carlsbad, CA) per manufacturer’s instructions. Quality and quantity of RNA were determined by using a Nanodrop ND- 1000 (Thermo Scientific, Wilmington, DE) and an Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA). RNA sequencing libraries were prepared using TruSeq RNA Sample Prep Kits (Illumina Inc., San Diego, CA) and samples sequenced by 2 × 100 bps paired-end sequencing on an Illumina HiSeq 2000 instrument at the Iowa State DNA facility
GEO Accession
GSM1611999