GSM3736850: 10_0265_Cmpd-125-6h_r2_BRD4; Homo sapiens; ChIP-Seq
Chromatin was isolated by the addition of lysis buffer, followed by disruption with a Dounce homogenizer. Lysates were sonicated and the DNA sheared to an average length of 300-500 bp. Genomic DNA regions of interest were isolated using (6 µg of) antibody against BRD4 (Bethyl, lot number: A301-985A100-6). Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit and sequenced on a NextSeq 500 at 75-bp in paired end mode.
GEO Accession
GSM3736850