GSM4120092: Ctrl1; Mus musculus; RNA-Seq
Mice were euthanized and the aorta (from descending arch to the renal bifurcations) was rapidly excised and placed in ice cold PBS.  The adventitia and endothelium were gently removed to create as pure a population of medial smooth muscle cells as possible.  Aortae were individually snap frozen in liquid nitrogen and then homogenized with a Bertin Minilys Personal Homogenizer (Bertin Instruments) in a 1.5ml microfuge tube containing 1ml of Trizol.  Total RNA was isolated according to instructions by the manufacturer and then samples were treated with DNAse I for 15 minutes at 37C.  DNAse I was removed by phenol/chloroform extraction following by standard sodium acetate/ethanol preciptation.  Samples were resuspended in RNAse-free water and submitted to the University of Rochester Genomics Research Core for quality assessment and library construction. Illumina TruSeq Stranded mRNA
GEO Accession
GSM4120092