GSM3536226: CD4 T cell wildtype rep3; Mus musculus; RNA-Seq
polyadenylated RNA from cells activated for 48 hours (0, 24, and 48 hours for CD4 T cells) was selected by using Sera-Mag oligo-(dT)14 (GE Healthcare), and reverse-transcribed to cDNA using RT primer (RT_1). The complementary (2nd) strand of cDNA was synthesized using the Klenow fragment (exo-, NEB) and extension primer (Ext_primer_1). Barcoded libraries, amplified by PCR from the double-stranded cDNA pool, were submitted for high throughput sequencing.
GEO Accession
GSM3536226