GSM2478111: IPF_3; Homo sapiens; RNA-Seq
The minced cleaned tissue was then incubated in DMEM/F12 (Life Technologies) containing 2 mg/ml Dispase (Corning), incubated at 4°C with rocking for 1 hour, and incubated for another 30 minutes at 37°C. Further elastase/trypsin digestion was then accomplished by the addition of an equal volume of Ham’s F12 medium containing 5 U/ml elastase (Worthington Biochemical Corp.) and 0.125% trypsin/EDTA (Corning). The reaction was arrested by addition of HBSS containing 0.01% HEPES, 0.5 M EDTA, and 0.02% FBS. Triturated tissue solution was subjected to a final protease digestion of 1 volume DNase I for 15 minutes at 37°C. Cells were plated overnight on collagen-coated plates in bronchial epithelial growth medium (Lonza) and harvested by mild trypsin digestion. Sequencing libraries were prepared using the Nextera XT DNA Sample Preparation Kit (Illumina).
GEO Accession
GSM2478111