GSM2476219: Ark1.Inactive_Mitosis_1; Schizosaccharomyces pombe; OTHER
Crosslinked chromatin was digested by DpnII. The sticky ends were filled in by Biotin-14-dATP and the other dNTPs. Proximal ligation was performed in diluted conditions, followed by DNA recovery. Crosslinked chromatin was immunoprecipitated with anti-HA antibody. NEBNext Ultra DNA Library Prep kit for Illumina and NEBNext Multiplex Oligos for Illumina (Index Primers Set 1) were used for adapter ligation and indexing. Indexed Hi-C library was purified by double selection of SPRI selection beads.
GEO Accession
GSM2476219