Comparative sequence analyses of genome and transcriptome reveal novel transcripts and variants in the Asian elephant Elephas maximus
Genomic DNA from total lymphocytes was isolated from peripheral blood (PBMCs) using PureLink genomic DNA isolation kit. For whole genome sequencing, a paired end library with 400-500 bp insert size and a matepaired library with 2-3 kb insert size were prepared. The paired end (PE) library was prepared using Illumina Trueseq library kit (as per manufacturer protocol). The mate pair library was prepared using the Illumina Nextera Mate-Pair library kit (as per manufacturer protocol). Bioanalyzer plots were used at every step to assess fragmentation sizes and final library sizes.