GSM3683718: Stromal Young Rep 2; Mus musculus; RNA-Seq
Sorted cells were pelleted and flash frozen on dry ice. RNA was extracted from the pellets using the RNeasy Mini Kit (QIAGEN) following the manufactural instruction. Libraries for RNA-Seq were prepared with KAPA Stranded mRNA-Seq Kit (Roche). The workflow consists of mRNA enrichment, cDNA generation, and end repair to generate blunt ends, A-tailing, adaptor ligation and PCR amplification. Different adaptors were used for multiplexing samples in one lane.
GEO Accession
GSM3683718