RNA-Seq of Chloridea virescens: adult male antennae
Adult male Chloridea virescens were sacrificed by freezing at -80C. Antennae were subsequently removed from a single male just prior to RNA isolation. 2 antennae (one pair) were used for extraction. RNA was isolated according to Trizol protocol, then processed on RNEasy micro columns for clean up. RNA was quantified using Bioanalyzer and Qubit, the sent to the NY Genome Center for library preparation and sequencing. Libraries were prepared using the Ovation RNA-Seq System for preparing amplified cDNA from total RNA followed by Illumina TruSeq Nano DNA Sample Preparation. 125bp paired-end sequencing was performed on an Illumina HiSeq 2500.