GSM2563208: SCR_CONTROL; Homo sapiens; Bisulfite-Seq
Nuclei isolated from exponentially growing cells were lysed and treated with M.CviPI GpC methyltransferase. Genomic DNA was isolated by standard phenol-chloroform extraction and ethanol precipitation. GpC enzyme (M.CviPI) treated DNA was fragmented to ∼200-bp, END-repaired (Epicenter), methylated adaptors ligated (Illumina), bisulfite-converted (Zymo EZ DNA methylation), and PCR-amplified
GEO Accession
GSM2563208