16S rRNA metagenomics human: stool
DNA was extracted using QIAamp Fast DNA Stool Mini Kit following the manufacturer's protocol with an added bead beating step. 16S rRNA amplicon sequencing library was prepared according to the 16S Metagenomic Sequencing Library Preparation (Part # 15044223 Rev. B) protocol for the Illumina MiSeq System. Indexing was done using the Nextera XT Index Kit v2 Set A. Sequencing was performed using Reagent Kit v3 (600-cycle) following manufacturers instructions to produce paired-end reads of 300 bp x2.