HCC1806-p63_H129-ChIPseq-1
50 million HCC1806 cells were crosslinked with formaldehyde (1%) for 10 minutes at room temperature, collected by scraping, and sonicated to yield ~300 bp DNA fragments using a Diagenode Bioruptor. ChIP was performed using 10 g of p63 H-129 (Santa Cruz Biotechnology, sc-8344) antibody. Input control samples were collected from sonicated samples prior to performing ChIP (NCBI Biosamples SAMN08683758 and SAMN08683761). ChIP-seq libraries were prepared as described previously in Marshall et al., Cell Reports, 2016. Illumina sequencing of ChIP-seq libraries was performed at the Vanderbilt VANTAGE core using a paired-end 75 bp protocol.