Illumina Genome Analyzer IIx paired end sequencing
Capture regions included all exons and 1.5 kb upstream regions of 336 target genes togetehr with over 7300 intergenic 220bp regions equally spread over Ae. aegypti genome. Target genes include detoxification enzymes (cytochrome P450s, glutathione S-transferases, carboxylesterases, UDP-glycosyltransferases) together with other enzymes potentially involved in insecticide biodegradation pathways and insecticide target proteins.
SureSelect® target enrichment system (Agilent Technologies)