Selective whole genome amplification of P. gaboni: Whole blood sample from Pan troglodytes
Venipuncture derived peripheral blood was mixed 1:1 (vol/vol) with RNAlater, transported at ambient temperatures and stored at -80C. Total extracted DNA was digested (MspJI, FspEI) and subjected to selective genome amplification using a Laverania specific SWGA primer set.  50 ng of the resulting product was amplified by selective amplification using a second Laverania specific SWGA primer set.  The 2nd round product was bead purified and prepared for illumina sequencing using the TruSeq PCR free kit.