GSM1611743: bursa_K4_L72_inf_10_1; Gallus gallus; ChIP-Seq
About 30 mg bursa samples were digested with micrococcal nuclease to obtain mononucleosomes. MNase digestion was followed by end-repair with PNK and Klenow enzymes (NBE, Ipswich, MA, USA) and ChIP with the specific antibody (H3K4me3: Millipore, Cat. #17-614; H3K27me3: Abcam, Cat. # ab6002). This was followed by addition of 3’ adenine, Illumina adaptor ligation, PCR amplification (17 cycles) and size-selection (~ 150 bp), cluster generation and sequencing on the Illumina Hi-Seq 2000.
GEO Accession
GSM1611743