GSM1694659: 1L; Glycine max; RNA-Seq
Total RNA was extracted using Trizol reagent (Sigma). Using Oligo(dT) magnetic beads adsorption to purify mRNA, and then use Oligo(dT) as primer to synthesize cDNA. At least 10 µg of total RNA (≥300 ng/µL) was extracted from samples for Solexa sequencing. Sample preparation, library construction, sequencing, and bioinformatics analysis were prepared for sequencing using standard Illumina protocols. The samples were sequenced by an Illumina HiSeq2000 platform, and 35 bp paired-end reads were produced.
GEO Accession
GSM1694659