GSM4670175: MDA-MB-231_CSCs_ KDM1A shRNA_2; Homo sapiens; RNA-Seq
CSCs transduced with lentiviral particles expressing either control shRNA or KDM1A shRNA vector and total RNA was isolated using RNeEasy mini kit using manufacturer's protocol (Qiagen, Valencia, CA). Total RNA samples about 1-2 micro grams were used for sequencing library preparation according to Illumina TruSeq Total RNA Sample Preparation Guide (Illumina Cat. #: RS-122-2201). Each library was bar-coded and then pooled for cluster generation and sequencing run with 100bp single-end (SE) sequencing protocol.
GEO Accession
GSM4670175