GSM3605133: LV1264_explant_RNA; Homo sapiens; RNA-Seq
RNA was isolated from homogenized left ventricle using the TRIzol Reagent (Thermo Fisher Scientific). RNA samples were DNase-treated with the TURBO DNA-free Kit (Thermo Fisher Scientific), and then RNA quality was assessed via 2100 Bioanalyzer (Agilent) and an RNA 6000 Nano Kit (Agilent). RNA-seq libraries were generated via the TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat kit (Illumina), according to manufacturer protocols. RNA-seq libraries were subjected to additional purification steps to remove remaining high molecular weight products.
GEO Accession
GSM3605133