RNA-sequencing of lymphoblastoid cell lines from the 1000 Genomes
RNA-sequencing of lymphoblastoid cell lines from the 1000 Genomes
Total RNA was extracted from cell pellets using the TRIzol Reagent (Ambion). The pellets had been frozen at ECACC without any additives like RNAlater or Trizol. In Geneva they were thawed, 1mL of trizol was added in each sample, and the samples were transferred to eppendorf tubes. The rest of the protocol followed the manufacturer's guidelines. No DNase treatment was done to the RNA samples. Sample processing for sequencing was done in a random order in each of the seven laboratories. Library preparation was done with TruSeq RNA Sample Prep Kit v2 using the high-throughput protocol.
Experimental Factor: population
GBR
Experimental Factor: laboratory
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