Normal143_OncoVar
Simultaneous fragmentation and adaptor ligation was performed on 50 ng gDNA by using the Nextera DNA Library Preparation kit, and purified by the Agencourt AmpureXP Purification System. Hybridization capture of pooled indexed libraries was performed using an Agilent SureSelect Custom DNA kit for NCI Oncovar V4, which targets 2.93 Mb of exonic sequence in 564 genes found to be mutated in diverse solid tumors. In addition, xGen Blocking Oligos specific to Nextera library adaptor sequences were used during hybridization.
alignment_software
bwa-mem 0.7.10