GSM2477834: P30-C57_rep3; Mus musculus; RNA-Seq
Retinas were removed, flash frozen on dry ice, and RNA was harvested using Nucleospin RNA Plus kit, which includes DNase treatment. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for directional next-generation sequencing using a modified TruSeq RNA Sample Preparation Kit protocol as described (Brooks et al. 2012). The protocol utilizes the labeling of the second strand of cDNA with uracil, which allows the degradation of this strand prior to sequencing
GEO Accession
GSM2477834