GSM4671069: SCoV_XL1_Exp2; Homo sapiens; RIP-Seq
Following UV crosslinking, cells were then lysed on the tissue culture plate by adding 1 ml of RAP lysis buffer (10 mM Tris pH 7.5, 500 mM LiCl, 0.5% dodecyl maltoside (DDM), 0.2% sodium dodecyl sulphate (SDS), 0.1% sodium deoxycholate, EDTA-free Protease Inhibitor Cocktail (Thermo Fisher Scientific) and Murine RNase Inhibitor (New England Biolabs)). Lysates were then collected and flash-frozen in liquid nitrogen for storage at -80°C. RAP-MS was carried out as described in Munschauer et al., Nature (2018). eCLIP and sequencing of crosslinked RNA fragments of RAP-MS purified proteins were carried out as described in the methods section of our manuscript "A direct RNA-protein interaction atlas of the SARS-CoV-2 RNA in infected human cells". eCLIP-seq (Nostrand et al. Nature Methods (2016)).
GEO Accession
GSM4671069