GSM1615624: GW3965 rep1; Mus musculus; RNA-Seq
The neurospheres were washed twice with cold-PBS before total RNA isolation with the RNeasy kit (Qiagen). The RNA integrity and concentration was checked using Qubit and 2200 TapeStation (Agilent) An aliquot of 0,5μg of RNA was used for cDNA synthesis. Illumina Truseq libraries were prepared using corresponding kit and protocols from Illumina.
GEO Accession
GSM1615624