GSM1937686: L1210_55 scRNA-seq; Mus musculus; RNA-Seq
Single cells were flushed from the device with 5 ul of PBS directly in to 5 ul of 2X TCL lysis buffer (Qiagen) resulting in a total volume of 10 ul of single-cell lysate. These samples were immediately frozen on dry ice and subsequently stored at -80°C prior to library preperation and sequencing. Libraries were constructed using the Smart-Seq2 protocol for single cells.
GEO Accession
GSM1937686