GSM2818527: LNCaP shCON CDT Rep 2; Homo sapiens; ATAC-seq
ATAC-Seq was performed as previously described (Buenrostro et al 2015). Briefly, cells plated and treated as indicated by the condition.  Next, 75,000 cells were collected and lysed in ATAC lysis buffer.  Cells were then re-suspended in the transposition reaction mix, and the transposition reaction incubated at 37oC for 30 minutes. Transposed DNA was purified using AMPure purification methods as specified by the manufacturer.  Purified DNA was then amplified utilizing custom barcoded Nextera primers as (previously listed and available in  (Buenrostro et al 2015)).  Library size and quality was validated using a Bioanalyzer and libraries sequenced on a NextSeq 500
GEO Accession
GSM2818527