nhl-2 20°C small RNA
Total RNA was collected from Bristol N2 (wild-type) and nhl-2(ok818) mutants that were grown to gravid adulthood at either 20°C or 25°C on OP50 E. coli. We isolated small RNAs and prepared libraries for Illumina sequencing using a previously described cloning strategy (Gu et al., 2011, Tu et al., 2015). The only minor modification to these protocols was that we used 0.1X the normal amount of Tobacco Acid Pyrophosphatase (enabling us to capture a greater proportion of miRNAs, while still sufficiently recovering 22G-RNAs).