Illumina MiSeq paired end sequencing
Extraction of total DNA for generation of metagenomic libraries.
DNA extracts were prepared from approximately 200 mg of faeces. Selective lysis of higher eukaryotic cells was performed using the MolYsis selective kit (Molzym), followed by bacterial lysis using lysozyme, mutanolysin (Sigma) and lysostaphin.DNA was purified using the MasterPure complete kit (Epicentre). Shotgun DNA libraries were subsequently prepared using the KAPA HyperPrep kit (KAPA Biosystems).