Illumina HiSeq 2000 paired end sequencing; Swine DNA methylation study using RRBS : FAANG
DNA methylation analysis
EZ DNA Methylation™ Kit
assay type
methylation profiling by high throughput sequencing
sample storage
frozen, -70 freezer
sample storage processing
cryopreservation in liquid nitrogen (dead tissue)
sampling to preparation interval
12
extraction protocol
ftp://ftp.faang.ebi.ac.uk/ftp/protocols/samples/KU_SOP_Preperation_of_Genomic_DNA_from_Small_Samples_20170516.pdf
library preparation location
Shenzhen, China
library preparation location longitude
114.0683000
library preparation location latitude
22.5455400
library preparation date
2013-08-20
sequencing location
Shenzhen, China
sequencing location longitude
114.0683000
sequencing location latitude
22.5455400
sequencing date
2013-08-20
experiment target
DNA methylation
library selection
RRBS
bisulfite conversion protocol
http://www.zymoresearch.com/downloads/dl/file/id/197/d5007d.pdf
pcr product isolation protocol
https://www.epigenesys.eu/images/stories/protocols/pdf/20160127163832_p70.pdf
bisulfite conversion percent
99.6
restriction enzyme
MspI
min fragment size selection range
40
max fragment size selection range
220