TSV resistance Litopenaeus vannamei
RNA-seq library preparation and sequencing was performed according to HudsonAlpha Genomic Services Lab (Huntsville, AL, USA) .  The cDNA libraries of TSV-resistant and susceptible shrimp were separately synthesized by  2-4 *g of starting DNase-treated total RNA following the protocols of the Illumina TruSeq RNA Sample Preparation Kit (Illumina) as described in the TruSeq Protocol. The libraries were amplified with 15 cycles of PCR and contained TruSeq indexes within the adaptors, specifically indexes 1–4. The final libraries had an average fragment size of ?270 bp and final yields of ?400 ng. After KAPA quantitation and dilution, the libraries were clustered 4 per lane and sequenced on an Illumina HiSeq 2000 instrument with 100 bp paired end (PE) reads.