GSM1931286: Hippocampus_High-CPF_smRNA2; Rattus norvegicus; miRNA-Seq
Total RNA including small non-coding RNA was isolated from the CA1 region of rat hippocampus using miRNeasy kit (Qiagen, Valencia, CA) according to the manufacturer’s protocol. Small RNA libraries were constructed using the TruSeq small RNA library preparation kit (Illumina, San Diego, CA) according to the manufacturer’s recommended protocol.  1 ug of total RNA were ligated to 3’ and 5’ adaptors containing bases targeting small RNAs and sequencing primer sequences, respectively.  The enrichment of adaptor-ligated small RNA molecules was achieved by reverse transcription followed by limited-cycle polymerase chain reaction.  Sample-specific barcode tags were incorporated into the small RNA libraries during the PCR process.  The quality and concentration of newly constructed libraries were assessed with Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).  Small RNA libraries constructed from the same type of tissue were pooled together in equimolar amounts and separated on 6% Novex Tris-Borate-EDTA gels (Life Technologies, Grand Island, NY) for size selection.  Gel-purified sequencing libraries were quantified using KAPA Library Quantification Kit (Kapa Biosystems, Woburn, MA).  Small RNA sequencing libraries were sequenced on the Illumina HiScanSQ according to the manufacturer's protocols.
GEO Accession
GSM1931286