16S rRNA gene sequencing of marine bacteria and archaea
The 16S rRNA gene hypervariable regions V4-V5 were amplified with primers 515F-Y and 926R as recommended by Parada et al. (2016), multiplexed following the Earth Microbiome Project as in Caporaso et al. (2012). PCR prodcuts were cleaned with an ENZA Cycle Pure Kit, pooled at equimolar proportions, and gel extracted to remove non-target bands. Amplicons were sequenced on an Illumina MiSeq using PE250 v2 chemistry and custom sequencing primers at the University of Montana Genomics Core.