GSM1695742: Beta-Catenin CaCo2; Homo sapiens; RIP-Seq
Cell nuclei were isolated, nuclear lysate was prepared and incubated overnight with 5 µg of beta-catenin antibody (Bethyl A302-012A) .Afterwards, the RNA-protein complexes were immunoprecipitated with protein G magnetic beads and the RNA fraction was extracted using Trizol reagent. Library construction was prepared following the manufacturer's recommendation (TruSeq RNA Sample Preparation kit, Illumina RS-122-2001)
GEO Accession
GSM1695742