GSM2475121: Tet_m6A_SMRTseq_replA; Tetrahymena thermophila; OTHER
Tetrahymena SMRT-seq protocol: Macronuclei were isolated from vegetative Tetrahymena cells through differential centrifugation. Genomic DNA was then purified through phenol:chloroform extraction and ethanol precipitation. Subsequently, the DNA was sheared and ligated to SMRTbell adapters for sequencing on 21 SMRTcells using P5-C3 and P6-C4 chemistry, according to manufacturer's instructions. SMRT-seq; Standard Pacific Biosicences SMRT-seq library construction protocol
GEO Accession
GSM2475121