Iso seq of Brassica juncea line Varuna
RNA was isolated from the pooled sample of seedling, leaf, inflorescence with developing siliqua and seed. The pooled RNA sample was considered to represent most of the expressed genes in B. juncea plant. Two different transcriptome libraries, one of size range 0.5-1 kb and the other one of 1-2 kb were sequenced separately using P6-C4 chemistry on PacBio RSII system.