GSM4219526: MCF10CA1a_cl1_NT_60_S9_pool1; Homo sapiens; RNA-Seq
RNA was isolated using the RNeasy mini kit (Qiagen) according to the manufacturer's protocol and treated by DNAse Ribosomal depletion was performed with the Ribo-zero gold kit (Epicentre). Libraries were performed from 20 ng ribosomal depleted RNA with the NEXTFLEX® Rapid Directional RNA-Seq Library Prep Kit (BIOO-Scientific). Libraries were sequenced using an Illumina NextSeq 500 platform (flow cell highoutput V2) and a 75 bp paired-end sequencing with approximately 30-35 million reads per sample
GEO Accession
GSM4219526