GSM2478350: SM5_N5; Homo sapiens; ncRNA-Seq
Total RNA was extracted from exosome pellets using the miRNeasy Micro Kit (Qiagen) Small RNA libraries were prepared and amplified using the NEBNext small RNA Library Prep Set (New England BioLabs). Amplified libraries were resolved on a 10% polyacrylamide gel for size selection. The 140 to 160 nucleotide bands corresponding to adapter-ligated constructs derived from the 21 to 40 nucleotide RNA fragments were excised and recovered in a DNA elution buffer. The average size distribution of each library was determined using Agilent Bioanalyzer with High Sensitivity Chip Kit (Agilent) and quantified on ABI 7900HT Fast RT-PCR instrument using the KAPA Library Quantification kit (Kapa Biosystems). Each library was adjusted to final concentration of 2 nM, pooled and sequenced on an Illumina HiSeq 2000 sequencer for single-read 50 cycles.
GEO Accession
GSM2478350