GSM3684243: LM2; Homo sapiens; RNA-Seq
Whole blood was collected in Tempus RNA tubes and stored at -80°C until RNA extraction, which was performed for all samples simultaneously by the Stanford Functional Genomics Facility. RNA quantification and quality assurance were performed on an Agilent 2100 Bioanalyzer, and library construction was completed using the Illumina preparation kit according to the manufacturer's instructions. Libraries were PolyA-enriched and followed by depletion of globin transcripts and ribosomal RNA. Paired-end sequencing of 76 base pair fragments was performed on the Illumina Nextseq, with an average coverage of 53 million reads per sample.
GEO Accession
GSM3684243