GSM2563525: Dunaliella salina RNA-seq; Dunaliella salina; RNA-Seq
Trizol reagent (Invitrogen, CA, USA) was used to extract total RNA as manufacturer's instructions. RNA quantity and purity were determined by Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0. Approximately 5 ug of total RNA for each sample was used to isolate mRNA by magnetic beads with poly-T oligo attached (Invitrogen). The purified mRNA was fragmented into small pieces by divalent cations under elevated temperature. The cDNA library was prepared by reverse-transcription in accordance with instructions of the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (±50 bp). The paired-end sequencing was performed on an Illumina HiseqTM2000 platform (LC Sciences, USA) following the vendor's instruction.
GEO Accession
GSM2563525