GSM1695866: Thy5 sample 1; Homo sapiens; RNA-Seq
The Trizol method (Mirneasy RNA extraction mini kit, Qiagen, Valencia, CA) was used to extract RNA from all samples. The Ovation RNA‑Seq System V2 (NuGen, San Carlos, CA) was used to convert 6-10 ng of RNA into amplified cDNA (linear amplification). The cDNA was sheared using a S220 focused ultrasonicator (Covaris, Woburn, MA) to generate an average fragment size of 350 base pairs. 900 ng of sheared cDNA was converted into sequencing libraries using the Encore Rapid DR Multiplex kit (NUGen)
GEO Accession
GSM1695866