GSM4669876: THP-1_AML_exposed_to_osteoblasts_2; Homo sapiens; RNA-Seq
RNA isolation, and cDNA preparation were carried out following standard protocols. Total RNA from cortical bone (femurs of 8-10-week old mice, cleaned, flushed and centrifugated 20 seconds at 10.000g's to remove bone marrow) was extracted using TRIzol (Invitrogen) followed by RNA clean-up using PureLink RNA Mini Kit (Ambion, Invitrogen). mRNA was reversed transcribed using random hexamers RNA-to-cDNA kit (Takara).
GEO Accession
GSM4669876