GSM4057881: 48hpab_1; Danio rerio; RNA-Seq
For RNA-seq, RNA was isolated from the laser microdissected samples using the miRNeasy micro Kit (Qiagen) combined with on-column DNase digestion (DNase-Free DNase Set, Qiagen) to avoid contamination by genomic DNA. RNA and library preparation integrity were verified with a BioAnalyzer 2100 (Agilent) or LabChip GxTouch 24 (Perkin Elmer). 20 ng of total RNA was used as input for PolyA enrichment (NEXTflexTM PolyA Beads) followed by library preparation using NEXTflexTM Rapid Directional qRNA-SeqTM Kit (Bioo Scientific Corp). Sequencing was performed on the NextSeq500 instrument (Illumina) using v2 chemistry, resulting in average of 38M reads per library with 2x75bp paired end setup.
GEO Accession
GSM4057881