WES of Homo sapiens:FCDII brain
First, the qualified genomic DNA sample is randomly fragmented into fragments with a base pair peak of 150 to 200bp, and then adapters are ligated to both ends of the resulting fragments. The adapter-ligated templates are purified by the AgencourtAMPure SPRI beads and fragments with insert size about 200bp are excised. Extracted DNA is amplified by ligation-mediated polymerase chain reaction (LM-PCR), purified,and hybridized to the SureSelectBiotinylated RNA Library (BAITS) for enrichment.Hybridized fragments are bound to the strepavidin beads whereas non-hybridized fragments are washed out after 24h. Captured LM-PCR products are subjected to Agilent 2100 Bioanalyzer to estimate the magnitude of enrichment.