GSM2824217: DHX36-KO Riboseq 1; Homo sapiens; OTHER
Cells were lysed in extraction buffer and lysate were titruated 6 times through a 26 G needle. After Rnase treatment, ribosomes were purified by ultracentrifugation through a sucrose cushion. Ribosome-bound RNA was isolated with Trizol Preadenylated, barcoded 3´adapter oligonucleotide was ligated followed by ligation of a 5´adapter. Ligation products were reverse transcribed and amplified by PCR.
GEO Accession
GSM2824217