GSM2476707: aP60_5_01; Mus musculus; RNA-Seq
[Bulk Cell RNA-seq (Sample 001-014)] Total RNA from approximately 3-5 × 104 sorted β-cells was extracted by the RNeasy Micro Kit (Qiagen, 74004) followed by mRNA purification using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490). [Bulk Cell RNA-seq (Sample 001-014)] Strand-specific libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB #E7420) following manufacturer’s instructions. [Single Cell RNA-seq (Sample 015-950)] cDNA synthesis and amplification were performed following the Smart-seq2 protocol. [Single Cell RNA-seq (Sample 015-950)] The libraries were prepared with 2 ng cDNA using the TruePrep DNA Library Prep Kit (Vazyme, TD502).
GEO Accession
GSM2476707