GSM1613286: LNCaP DHT + Bicaltamide 24h; Homo sapiens; RNA-Seq
Total RNA was extracted using ISOGEN reagent. First strand cDNA was produced using Superscript III (Invitrogen).  We chose AMPure RNAClean XP (Beckman Coulter) for the purification of RNA/DNA hybrids. After biotynation, the cap-trapping process, quality control was performed by measuring concentration (Oligreen). Poly-A tailing/blocking is carried out prior to HeliScope sequencing according to the instructions found in the HeliScope(TM) Low-Volume Sample Loading Protocol (LB-017 Rev. 01). .
GEO Accession
GSM1613286