GSM1614163: PAPERCLIP for HeLa cells, replicate 1; Homo sapiens; RIP-Seq
Sample preparation, immunoprecipitation, SDS-PAGE and RNA extraction were adapted from standard HITS-CLIP (Moore et al. 2014).  Mouse monoclonal anti-PABP (Sigma, P6246) was used for immunoprecipitation. The sequencing library was constructed using BrdU-CLIP method (Weyn-Vanhentenryck et al. 2014) with modification to improve sensitivity.  The library contains a 14-nt degenerate linker sequence at the 5′ end (6-nt random barcode followed by 8-nt sample multiplexing index).  The complete protocol is detailed in Supplemental Method.
GEO Accession
GSM1614163