ago1-25 parental
DNA libraries were prepared with the Ion Shear Plus and Ion Xpress Plus kits, amplified by 5 PCR cycles, and its quality assessed with a Bioanalyzer 2100 using the DNA high sensitivity chip (Agilent Technologies, Santa Clara, California, USA). Sequencing templates were created with an Ion OneTouch and an Ion ES instrument using the Ion PI OT2 200 kit v3, and analyzed with a Qubit 2.0 fluorometer using the Ion Sphere Quality Control kit. Sequencing was performed with the Ion PI 200 kit v3 in an Ion PI chip v2.