Development and immunity of Micrioplitis bicoloratus in host Spodoptera litura
Eggs, Embryo, 1st larvae, 2nd larvae and 3rd larvae were prepared for total RNA isolation  using an RNeasy Plus Universal Mini Kit (QIAGEN, Maryland, USA), which is specific for genome DNA elimination, according to the manufacturer’s instructions. The concentration of each RNA sample was determined by measuring OD at A260/A280 using the NanoDrop 2000 and running agarose gel. High quality samples (with an A260/A280 ratio >2.0, A260/A230>2.0, concentration>500 ng/ul) showing 28S and 18S RNA bands clearly were stored at -80°C until use.