GSM1932135: O+1bp sgRNA replicate 1; Homo sapiens; OTHER
Genomic DNA was extracted from the sorted cells using QIAamp DNA blood mini kit (QIAGEN) The library construction follows Illumina’s TruSeq method. Briefly, sgRNA sequences were amplified by 2 rounds PCR with the first pair of primers, ACACTCTTTCCCTACACGACGCTCTTCCGATCT TCTACCTGCTCAACCTGGCC (forward) and GTGACTGGAGTTCAGACGTGTGCTCTTCCGATC AAGTCCCACTGGGCGGC (reverse), followed by amplification with the second pair of primers, AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGA (forward) and CAAGCAGAAGACGGCATACGAGATNNNNNNGTGACTGGAGTTCAGACGTG(reverse, where NNNNNNN indicates index sequences). TruSeq
GEO Accession
GSM1932135