GSM4670267: sgIfngr1 [CGATCAGT_ATAGCCTT]; Mus musculus; RNA-Seq
Each CNS was isolated per mouse. A cell suspension was generated by papain digestion, neutralization, and 70um filtration of cell extract. Cells were centrifuged, resuspended in 30% Percoll, centrifuged, and washed in PBS. Cells were sorted by flow cytometry based on CD11b-low, CD45+, Ly6c-low. Libraries were prepared by the Broad Institute according to Smart-seq 2 protocol as described (Picelli et al., 2014)
GEO Accession
GSM4670267