Shotgun genome sequencing of Neofusicoccum parvum (isolate UCRNP2)
Genomic DNA was extracted mycelia growing on PDA plate.  A single DNA library was prepared from 5 ug of total genomic DNA that was randomly fragmented using the Bioruptor sonicator (Diagenode). The library was prepared with the NEB Next DNA Sample Prep Master Mix according to manufacturer's instructions (New England Biolabs, MA USA).  Fragments were purified using the Nucleotrap kit (Clontech) and eluted in 30 ul elution buffer and PCR-enriched using 14 PCR cycles. Paired end adapters and PCR primers were obtained from Illumina. Library quality and quantity were confirmed before sequencing using a Bioanalyzer (Agilent).