Bacterial 16S rRNA genes from digestate of biogas plant nr.7 replicate b
Liquid pig manures, fermenter and post-fermenter materials and digestate samples were taken from eight full-scale mesophilic digester BGPs located in Lower Saxony. Total community (TC-)DNA was extracted using the spin kit for soil (MP Biomedicals, Heidelberg, Germany) and aliquots of the DNA (diluted 1:5 with 1x TE buffer pH 8.0) were applied in 454-pyrosequencing (performed at BIOCANT, Portugal). Briefly, the hypervariable V3-V4 regions of 16R rRNA genes were amplified using the primers 338F and 802R fused to the 454 A and B adaptors, respectively.