GSM4121365: TOP2A ChIPSeq; Mus musculus; ChIP-Seq
To prepare chromatin, mouse thymus was extracted from 4-6-week old mice, washed twice with cold PBS and mechanically disaggregated into single cells in 1ml EDTA buffer (100 mM NaCl, 1 mM KH2PO4, 3 mM KCl, 10 mM Na2HPO4, 1 mM EDTA). Thymocytes were then washed twice and resuspended again in EDTA buffer. Cells were fixed by adding formaldehyde to a final concentration of 1% and incubated at 37ºC for 10 min. Libraries were prepared by ChIPmentation as described by Schmidl et al. 2015 using NEBNext HF Polymerase and sequenced using Illumina NextSeq 500.
GEO Accession
GSM4121365