GSM3004396: OE_2; Komagataella phaffii; RNA-Seq
Pellets from the samples collected during the exponential growth phase were resuspended in 1 mL TRI Reagent (Sigma, USA). Cells were disrupted by glass beads using a ribolyser (MP Biomedicals, USA) for 40 sec at 5.5 ms-1. RNA was extracted by chloroform and precipitated with isopropanol. After washing twice with 75% ethanol, RNA was dissolved in nuclease-free water. After DNase treatment (Invitrogen, USA), concentration of RNA was measured by Nanodrop and quality of the samples was verified with the Agilent Bioanalyzer 2100 using the RNA 6000 Nano Assay kit (Agilent Technologies, California). RNASeq analysis was performed with poly(A)-enriched mRNAs at Vienna Biocenter Core Facilities GmbH (AT). cDNA libraries were constructed from these poly(A)-enriched mRNAs and sequenced by Illumina HiSeq v4.
GEO Accession
GSM3004396