GSM4059723: CWRU205 LP3a; Homo sapiens; RNA-Seq
For each stage of differentiation, confluent cells were harvested for RNA extraction using TRIzol. HBE-ALI cells were harvested similarly. RNA quality was confirmed by Nanodrop measurement of OD 260/280 and 260/230 ratios and stored at -80oC under ethanol. 1ug RNA was used for RNA-seq library preparation. RNA-seq libraries were prepared using the TruSeq RNA Sample Preparation Kit v2 (Low-Throughput protocol, Illumina).
GEO Accession
GSM4059723