GSM2476339: NRAS metastatic melanoma ATAC-seq [SKmel147]; Homo sapiens; OTHER
For all ATAC-seq libraries, 150k cells were harvested, tagmented with 5uL Nextera Tn5 Transposes from nextera kit (Illumina) for 30 min, amplified up to 10 cycles and purified essentially as previously described in ATAC protocols.. Purified libraries were then size selected on 2% agarose gel (150-700bp). Libraries were sequenced on Illumina Hi-Seq2500. For all ATAC-seq libraries, 150k cells were harvested, tagmented with 5uL Nextera Tn5 Transposes from nextera kit (Illumina) for 30 min, amplified up to 10 cycles and purified essentially as previously described in ATAC protocols.. Purified libraries were then size selected on 2% agarose gel (150-700bp). Libraries were sequenced on Illumina Hi-Seq2500.
GEO Accession
GSM2476339