GSM2824309: set2_viewpoint_1_replicate_4; Mus musculus; OTHER
The initial steps of the 4C procedure, as published before (van de Werken et al., 2012, Nature Methods (PMID 22961246)), remain unchanged.  Cells are cross-linked using 2% formaldehyde for 10 minutes at room temperature in PBS 10%FCS and nuclei are isolated, after which chromatin is digested with DpnII and subsequently ligated. After reversal of the cross-links, the DNA is purified and treated with the second restriction enzyme treatment (Csp6I). After a second re-ligation step, the sample is purified and a 4C PCR is performed. For mESCs we generated three biological replicates from individual dishes (~10x106 cells). For fetal livers, biological replicates were generated from individual embryos from a single pregnant mouse. We used barcoded primers to distinguish between replicates and tissues. 4C PCR
GEO Accession
GSM2824309