GSM2901540: H3K9me2_ibm1_Rep2; Arabidopsis thaliana; ChIP-Seq
For ChIP-seq, chromatin was sonicated and immunoprecipitated with the respective antibody; For RNA-seq, total RNA were isolated using TRIzol reagent, DNA and ribosomal RNA were removed using the Ribo-Zero rRNA Removal Reagents (Plant Leaf)(Epicentre, USA). ChIP-seq libraries and RNA-seq were prepared using NEBNext Ultra II DNA library prep kit for Illumina (E7645, New England Biolabs) and NEBNext Ultra Directional RNA library prep kit for Illumina (E7420, New England Biolabs) according to the manufacturer's instruction respectively.
GEO Accession
GSM2901540