Filling in the gap of human chromosome 4: single molecule real time sequencing of macrosatellite repeats in the fascioscapulohumeral muscular dystrophy locus.
BAC DNA was first purified by MoBio PowerClean Kit, sheared by centrifugation through g-Tube, and then concentrated by AMpure. SMRT bell library was constructed using DNA Template Prep Kit v2.0 and then analyzed by PacBio RS using DNA/Polymerase Binding Kit P5, Sequencing reagent v2.0, and a single SMRT cell v3 (P5-C3).