GSM3146090: DelB.L [Repli-seq epha4]; Mus musculus; OTHER
Genome-wide RT profiles were constructed as previously described. Briefly, cells were pulse labeled with BrdU for 2 hours and separated into early and late S-phase fractions by Propidium iodide staining and flow cytometry. BrdU-substituted DNA from 40k early and late S-phase cells was immunoprecipitated by anti-BrdU antibody (Becton Dickinson 347580). Sequencing libraries were prepared by NEBNext Ultra DNA Library Prep Kit for Illumina (E7370).
GEO Accession
GSM3146090