GSM4172947: DropSeq_WT2_Lin_c-Kit_4wk; Mus musculus; RNA-Seq
Bone marrow (BM) cells were isolated into cold phosphate-buffered saline (PBS), without Ca2+ and Mg2+, and supplemented with 2% bovine serum albumin (BSA) to generate single cell suspensions. Red blood cells (RBCs) were removed using ammonium chloride-potassium bicarbonate (ACK) lysis buffer, resuspended in PBS/2% BSA, and filtered through a 40μm cell strainer. Cells were sorted and inmediately underwent the indicated protocol.
GEO Accession
GSM4172947