GSM2396746: wt INF; Mus musculus; OTHER
Genomic DNA was isolated using the Dneasy Blood and Tissue Kit (Qiagen) according to manufacturer's protocol Three independent libraries with initially different enzyme digestion were constructed for each sample, which were termed "C", "C+mC" and "C+mC+hmC", respectively. For "C+mC" library construction, an aliquot of genomic DNA from each sample was first glucosylated and then digested with MspI. For the "C" or "C+mC+hmC" libraries, the genomic DNA was directly digested with HpaII or Msp!, respectively. The digested DNA from all threee libraries were ligated with biotinylated linker, fragmented, captured, digested to generate short sequence tags and ligated with sequencing linkers. HMST-seq
GEO Accession
GSM2396746