HaCaT_C2-input-ChIPseq-1
25 million HaCaT C2 (single cell clone of HaCaT parental cell line) cells were crosslinked with formaldehyde (1.5%) for 10 minutes at room temperature, collected by scraping, and sonicated to yield ~300 bp DNA fragments using a Diagenode Bioruptor. Chromatin was collected prior to performing ChIP and used as an input control sample for the ChIP-seq analysis. ChIP-seq libraries were prepared as described previously in Marshall et al., Cell Reports, 2016. Illumina sequencing of input libraries was performed at the Vanderbilt VANTAGE core using a single-end 75 bp protocol.