NextSeq 500 paired end sequencing; Altered expression of maize PLASTOCHRON1 enhances biomass and seed yield by extending cell division duration
Altered expression of maize PLASTOCHRON1 enhances biomass and seed yield by extending cell division duration
Plants for leaf growth monitoring were grown under growth chamber conditions with controlled relative humidity (55%), temperature (24°C day/18°C night), and light intensity (170 mmol per m2 per second photosynthetic active radiation at plant level) provided by a combination of high-pressure sodium vapor (RNP-T/LR/400W/S/230/E40; Radium) and metal halide lamps with quartz burners (HRI-BT/400W/D230/E40; Radium) in a 16h/8h (day/night) cycle. Total RNA was isolated with the guanidinium thiocyanate-phenol-chloroform extraction method using TRI-reagent (Sigma-Aldrich) Library preparation was done using the TruSeq RNA Sample Preparation Kit v2 (Illumina). In brief, poly(A)-containing mRNA molecules were reverse transcribed, double-stranded cDNA was generated and adapters were ligated. After quality control using a 2100 Bioanalyzer (Agilent), clusters were generated through amplification using the TruSeq PE Cluster Kit v3-cBot-HS kit (Illumina), followed by sequencing on an Illumina HiSeq2000 with the TruSeq SBS Kit v3-HS (Illumina).
Experimental Factor: wild type genotype
genotype
Experimental Factor: 6
age