Whole Genome Sequencing of E. coli isolate C227-11 (CCS)
We isolated DNA using a Qiagen DNEasy Blood and Tissue Kit per manufacturer instructions. The isolate was grown overnight in standard LB broth and the extraction was performed according to the kit instructions using 1 ml overnight culture per reaction and treating with proteinase K for 2 hours. The DNA was eluated in 100 ul AE buffer.