GSM1936675: KI_Bcell_Spleen_RNA-Seq; Mus musculus; RNA-Seq
Total RNA was used for library construction with the Illumina TruSeq strand mRNA Low Input Protocol RS-122-2101 protocol (Illumina, San Diego, CA) Total RNA from FACS-sorted CD4+ splenic or thymic T cells and IgM+ splenic B cells from female littermates was extracted using the RNeasy Mini Kit (Qiagen) For library construction, 0.1 - 1 µg of total RNA was subjected to mRNA capture with oligo-dT coated magnetic beads. The mRNA was fragmented, followed by random-primed cDNA synthesis, standard Illumina library prep with end-repair, adapter ligation and PCR amplification.
GEO Accession
GSM1936675