GSM4121366: Wild-type haploid MATa mRNA seq SPO 4h replicate 1; Saccharomyces cerevisiae; RNA-Seq
Cell pellets were washed once with sterile water and snap-frozen in liquid nitrogen. Total RNA was extracted using hot acid phenol and TE-SDS buffer, then precipitated in ethanol with 0.3 M sodium acetate before re-suspension in RNAse-free sterile water. At least 5 μg of total RNA was treated with DNAse and purified on column (Macherey-Nagel). At least 500 ng of purified total RNA was used as the inputs for the KAPA mRNA Hyper Prep kit (KK8580, Roche). Libraries were prepared according to manufacturer's instructions. After bead based clean up, libraries were sequenced on an Illumina HiSeq 4000 to an equivalent of 75 bases single-end reads, at a depth of approximately 20 million reads per library.
GEO Accession
GSM4121366