Metatranscriptome of Geodia parva from Karasik seamount
RNA was extracted with QIAGEN AllPrep DNA/RNA Mini Kit including on-column DNase treatment with the QIAGEN RNase-free DNase Set. RNA was treated with SUPERase In, quantity and quality were assessed by NanoDrop. Two technical replicates were pooled and cleaned with the QIAGEN MinElute Kit. DNA contamination was assessed by PCRs and RNA was treated with the Ambion DNA-free DNase treatment & removal kit. RNA quality was assessed by Experion, eukaryotic mRNA was removed with the Dynabeads mRNA DIRECT purification kit using the supernatant for further steps, cleaned with QIAGEN MinElute kit, quantity measured by Qubit assay, rRNA was removed with Illumina Ribo-Zero Epidemiology Kit and RNA was cleaned with QIAGEN MinElute kit.