GSM1931603: small RNA sequencing in ∆rrp6 dhp1-2 cells; Schizosaccharomyces pombe; ncRNA-Seq
RNA was extracted using the MasterPure™ Yeast RNA Purification Kit (epicentre) 21-25nt small RNAs were isolated by denaturing gel electrophoresis and after ethanol precipitation. Libraries were then constructed using the NEBNext Small RNA Library Prep Set for Illumina (NEB). Amplified DNA of 140-150bp was extracted from a 6% PAGE gel. Eluted DNA was ethanol precipitated overnight and resuspended in TE buffer. Libraries were analyzed using an Agilent 2100 BioAnalyzer (Agilent) and sequenced on the Illumina MiSeq platform.
GEO Accession
GSM1931603