GSM2398367: WT H3K4me2 ChIPseq-2; Mus musculus; ChIP-Seq
Crosslinked samples were fragmented using Covaris S220, followed by immunoprecipitation with the specific antibodies. ChIP DNA was subjected to library preparation with NEBNext Fast DNA Library Prep Set for Ion Torrent kit (New England Biolabs.). DNA was end-repaired, adapter-ligated, and size-selected with Agentcourt AMpure XP (Beckman Coulter Inc.) to median length of 300bp. Size-selected DNA was PCR amplified with Ion barcode adapter (Thermo Fischer Inc.) for 12 cycles, and purified with Agentcourt AMpure XP. High-throughput sequencing was performed using Ion Proton semiconductors sequencers (Thermo Fischer Inc.) according to the manufacturer’s instructions.
GEO Accession
GSM2398367