GSM2563724: 0 mg/ml curcumin treatment _12h  : replicate 2; Dictyostelium discoideum; RNA-Seq
At each time point, we harvested the cells directly into 1 mL of Trizol® (life technologies, CA, USA) and extracted total RNA according to the manufacturer’s recommended protocol. We performed two rounds of poly-A selection and fragmented 100 ng of the resulting mRNA into approximately 200 bases fragments. We prepared cDNA and the second strand. We prepared  two sets of Illumina multiplexed libraries. We prepared 48 individual libraries separately and deveided two sets. In each set, we added a unique barcode to each library at the final step of PCR amplification. We pooled equal amounts of DNA from each library and sequenced one pool per lane of a flow cell on Illumina Genome Analyzer II (read length = 50 bases).
GEO Accession
GSM2563724