GSM3003886: STAT1-competent carrier challenged 4; Mus musculus; RNA-Seq
Brains were isolated and stored in RNAlater solution (Thermofisher) at -80° C until further processing. Frozen tissue was transferred in Supplemented Homogenization Buffer (HB-S) and lysed with tissue lyser (Qiagen). After lysis of the tissues and before immunopurification, a small fraction of lysate of each sample (input) was set aside and total RNA was extracted with TRIzol (Sigma) reagent followed by the RNeasy plus Micro Kit's procedure (Qiagen). After immunopurification of the ribosome-associated RNAs, RNA quality was assessed on a 2100 BioAnalyzer (Agilent) and RNA amounts were quantified using the Qubit 2.0 Fluorometer (Life Technologies). The RNA integrity value (RIN) of the samples selected to be sequenced ranged between 7 and 9.2. RNA-samples were prepared using "TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold (Cat. No. RS-122-2301, RS-122-2302, and RS-122-2303) according to the manufacturer's protocol (Illumina). Samples were sequenced using the HiSeq 2000 (Illumina) (SE, 50 bp, ca. 30 Mio reads/sample)
GEO Accession
GSM3003886