UTSW MRSA 55
Genomic DNA was extracted from Staphylococcus aureus culture for a DNA concentration of 24.8 ng/uL. Sequencing libraries were generated using Pacific Biosciences SMRTBell Template Prep. protocols and fragmented using gTUBE shear protocols to 20kb fragment size. Final library size ranged : 3000bp - 30,000bp. Sequencing was carried out on PacBio RS Instrument for a total of 6 SMRT cells using P4-C2 chemistry. Total of 318,329 reads were obtained on MRSA 55 sample which gave average 954 X coverage.