Targeted sequencing of salivary gland tumor
DNA was extracted semi-automated (Maxwell16, Promega) from FFPE material, and  DNA was sonographically sheared (Covaris) into approximately 200-bp double-stranded fragments. Hereafter, adapters were ligated, and genomic regions of interest were enriched using complementary bait sequences. Following the enrichment, the targeted fragments were clonally amplified and sequenced with next-generation sequencing instrument (NextSeq 50 Illumina).