RNA-Seq of Mus musculus: male lung: Mouse with PCP after 8 days of cessation of anidulafungin treatment
Using PrepX mRNA Library kit (WaferGen) and Apollo 324 NGS automated library prep system, the depleted or isolated RNA was RNase III fragmented, adaptor-ligated and Superscript III reverse transcriptase (Lifetech, Grand Island, NY) converted into cDNA, followed by automated purification using Agencourt AMPure XP beads (Beckman Coulter, Indianapolis IN). 500 ng total RNA was used as input, and 13 cycles of PCR to introduce index and amplify the library were performed.