GSM2898318: arp6;brm Mnase-seq - rep1; Arabidopsis thaliana; MNase-Seq
Native chromatin from purified nuclei was digested with MNase. After lysing nuclei with 1% SDS, nucleosome associated DNA was purified using the MinElute kit (Qiagen), after RNase A and Proteinase K treatments. Fragments were size selected for those >400 bp using SeraPure magnetic beads. Libraries were prepared with the ThruPLEX DNA-Seq kit (Rubicon Genomics) using 25 ng of MNase-digested DNA. Libraries were sequenced on a an Illumina NextSeq500 with 76 bp paired-end reads.
GEO Accession
GSM2898318