Clone dilution pool Illumina paired-end shotgun sequencing for whole-genome haplotype resolution of individual G1-M
Indexed Illumina sequencing libraries were prepared in sets of 96 with the Nextera library preparation kit as previously described (A.Adey,H.G.Morrison,Asan,X.Xun,J.O.Kitzman,E.H.Turner,B.Stackhouse,A.P.MacKenzie, N. C. Caruccio, X. Zhang, J. Shendure, Rapid, low-input, low-bias construction of shot- gun fragment libraries by high-density in vitro transposition. Genome Biol. 11, R119 (2010).), followed by library pooling and size selection to 350 to 650 bp.