GSM3145915: abc.del.A6 [4C-seq]; Mus musculus; OTHER
4C-seq was performed as previously described with minor modifications. 10 million cells were fixed in 1% formaldehyde, and chromatin was digested using HindIII before proximity ligation, followed by DpnII after reverse crosslinking. 4C libraries were prepared using inverse-PCR primers that contain Illumina adapters and anneal to the pBACe3.6 bacterial sequences (forward primer: 5'-ATAAACTACCGCATTAAAGC; reverse primer: 5'-GGAGCCACTATCGACTACGC). Libraries were sequenced with 3-7 million mappable reads using 50bp single-end sequencing with Illumina HiSeq 2500.
GEO Accession
GSM3145915