GSM3737170: Cbfb-cKO_CD103-CD11b+_rep3; Mus musculus; RNA-Seq
Single cell suspensions from mouse intestine were prepared using EDTA and collagenase IV with agitations. Dendritic cells were flow-sorted into buffer TCL (QIAGEN) by FACS Aria and RNA were isolated using Agencourt RNAClean XP beads (Beckman Coulter). RNA libraries were prepared for sequencing applying scRNA-seq procedure with modofications
GEO Accession
GSM3737170