GSM2898004: F2 Control; Daphnia magna; Bisulfite-Seq
genomic DNA extracted from Daphnia magna whole organisms with the Qiagen DNeasy Blood & Tissue Kit Libraries were produced following the MethylC-Seq protocol (Urich MA, et al. Nat Protoc 2015). Briefly, genomic DNA was fragmented by sonication, end repaired and 3' Adenylated, ligated with methylated adpaters, size selected, bisufite converted with  ZYMO EZ DNA Methylation-Gold kit and PCR amplified with a last round of library size selection. Whole genome bisuflite sequencing  using the MethylC-seq protocol
GEO Accession
GSM2898004