Sample 177_nfp_ctrl_1 1x100bp Illumina sequencing
Three replicates from each of wt, nfp, and dmi3 plants were treated for one hour by replacement of the medium with modified Fahraeus medium with (+NF) and without (ctrl) 10-8 M Nod factors obtained from Sinorhizobium meliloti strain Rm1021 pRmE43 (pTE3:nodD1). After one hour of treatment the roots were excised, flash frozen, and processed using a Qiagen RNeasy Plant Mini kit. Sequencing libraries were prepared using the Illumina TruSeq RNA Sample Preparation Kit (mRNA protocol rev. A) and sequenced on an Illumina HiSeq 2000 system. For further experimental details see doi:10.1074/mcp.M112.019208.