genome analysis of Salmonella spp.: environmental
Bacterial DNA was extracted using a Qiagen DNeasy blood and tissue kit (Bio-Strategy, NZ) according to the manufacturers instructions for gram negative bacteria, with the final elution step modified to 200ľl sterile MilliQ water (Merck, NZ). Qubit DNA, RNA and protein assay kits (Life Technologies, NZ) were used to ensure DNA quality. DNA was stored at -20oC prior to sequencing at New Zealand Genomics Ltd, New Zealand. Sequencing, genome assembly, curation and annotation were conducted as in Grange et al (2016) doi: 10.1007/s00248-015-0721-5.