GSM1614025: CK_R; Ammopiptanthus mongolicus; miRNA-Seq
Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) from leaves and roots of A. mongolicus seedlings following the manufacturer’s procedure. Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer’s procedure. The quantity and purity of total RNA were analyzed using Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0. Approximately 1 ug of total RNA were used to prepare small RNA library according to protocol of TruSeq Small RNA Sample Prep Kits(Illumina, San Diego, USA). And then we performed the single-end sequencing (36 bp) on an Illumina Hiseq2500 at the LC-BIO (Hangzhou, China) following the manufacturer’s instructions.
GEO Accession
GSM1614025