GSM4121232: HSCs, [Ca2+]low-2; Mus musculus; RNA-Seq
One hundred  [Ca2+]high HSCs and  [Ca2+]low HSCs were sorted into RNA lysis buffer. The first strand of cDNA was synthesized by using PrimeScript RT reagent kit (TaKaRa Bio Inc.) and not-so random primers. Following the synthesis of the first strand, the second strand was synthesized by using Klenow Fragment (3', -5', exo-; New England Biolabs Inc.) and complement chains of not-so random primers. Using purified double-strand cDNA, the library for RNA-Seq was prepared and amplified using Nextera XT DNA sample Prep kit (Illumina Inc.). These prepared libraries were sequenced on Next-Seq system (Illumina Inc.), according to the manufacturer's instruction.
GEO Accession
GSM4121232