Maintainer line for Rajalaxmi at Panicle initiation
Leaf samples from maintainer line (B) for hybrid, Rajalaxmi, CRMS32B at panicle initiation stage were harvested and sequenced. The libraries were prepared by fragmenting the mRNA followed by first strand cDNA synthesis, second strand cDNA synthesis, adaptor ligation, 200 bp cDNA fragments isolation and amplification (18 cycles of PCR). Libraries were sequenced (2 x 100 bp) on Illumina HiSeq 2000.