GSM1881496: Huh-7.5 std.-CLIP miR-122 exp., LNA-122-1; Homo sapiens; RIP-Seq
Huh-7.5 cells were UV-irradiated.  AGO-cross-linked RNA was isolated with the Std. AGO-CLIP or CLEAR-CLIP protocol described in detail in the accompanying publication. AGO-cross-linked RNA was cloned by addition of pre-adenylated 3' linker and RNA 5' linker, then RT-PCR, as described in the published CLEAR-CLIP protocol. Products were amplified in a second round of PCR that added a 4 nt index (for multiplexing, see above) and 16 nt common primer sequence.  Raw files are demultiplexed on the basis of this index, but have not been further modified.
GEO Accession
GSM1881496