GSM3901317: EM_CD8-T cells; Homo sapiens; RNA-Seq
Human efffector memory CD8+ T cells were loaded to a microwell-based chip (sc-FTDseq) for mRNA capture by uniquely barcoded beads. The cells were lysed using three cycles of freeze-thaw . The single cell mRNA libraries were prepared using the DropSeq protocol (GSE63473), tagged with Illumina nextera single indexes (N701, N702, N703, N704, N705, N706). We used the same protocol as in scFTD-seq(GSE109535), where each mRNA captured was end-paired with an unique molecular index(UMI) and a cell barcode.
GEO Accession
GSM3901317