GSM3145796: Heart_cultured_donor_37; Homo sapiens; RNA-Seq
Total RNA from cultured and freshly isolated organ specific endothelial cells was purified using the RNAeasy Mini Kit (Qiagen) and residual DNA was removed by on-column DNase digestion. RNA quality was assessed with the Agilent RNA 6000 Nano Kit using the Agilent 2100 Bioanalyzer (Agilent Technologies). Only samples with RNA integrity number higher than 8 were kept for further analysis. RNA sequencing was performed on poly-A-enriched samples using Illumina TruSeq. Each library was barcoded using the Illumina adapters, and amplified with 13 cycles of PCR. Library concentrations were quantified using the Quant-it dsDNA Assay (Life Technologies, Carlsbad, CA). Libraries were normalized and pooled based on Agilent 2100 Bioanalyzer results (Agilent Technologies, Santa Clara, CA), and the pools were sequenced on an Illumina HiSeq 2500.
GEO Accession
GSM3145796