GSM1912808: DTP_TSA_H3K9me3_ChIPseq; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 15 min and quenched with 0.125 M glycine.  Chromatin was isolated by the addition of lysis buffer, followed by disruption with a Dounce homogenizer. Lysates were sonicated and the DNA sheared to an average length of 300-500 bp. Illumina sequencing libraries were prepared from the ChIP and input DNAs following standard enzymatic steps consisting of end-polishing, dA-addition, and adaptor ligation. The resulting DNA libraries were quantified and sequenced as 150 bp paired-end reads using Illumina’s HiSeq 2500. The average size of the sequenced fragments were around 380 bp.
GEO Accession
GSM1912808