rRNA of Bonneville Salt Flats: sample 29-1
The V4 region of the bacterial 16S rRNA gene was amplified with dual-indexed Illumina fusion primers with the 515F/806R primers. Amplicon concentrations were normalized and pooled using an Invitrogen SequalPrep DNA Normalization Plate. The pool was sequenced on an Illumina MiSeq v2 flow cell using a standard 500 cycle reagent kit. Base calling was performed by Illumina Real Time Analysis (RTA) software v1.18.54. Output of RTA was demultiplexed and converted to fastq files using Illumina Bcl2fastq v1.8.4.