16S amplicon sequencing of Argiope bruennichi: adult female hemolymph
Extracted DNA was amplified with primer pairs targeting the V4 region of the 16S rRNA (515f: 5-GTGYCAGCMGCCGCGGTAA-3, 806r: 5-GGACTACNVGGGTWTCTAAT-3 (Walters et al., 2015) coupled to custom adaptor-barcode constructs. PCR amplification and Illumina MiSeq library preparation and sequencing (V3 chemistry) was carried out by LGC Genomics in Berlin.