GSM3146456: PR8 infected 293T, AgoIP; Homo sapiens; RIP-Seq
Small RNA were extracted with the miRvana microRNA isolation kit (Thermo Fisher) or RNA co-immunoprecipitated with an Ago2 antibody for the AgoIP samples Small RNA processed with the TruSeq Small RNA Sample Preparation Kit (Illumina). Adapter-ligated RNA was reverse transcribed using SuperScript III (Life Technologies) and amplified using GoTaq green PCR master mix (Promega) with TruSeq 3′ indices.  DNA bands corresponding to ~145 bp were isolated by gel electrophoresis on 10% polyacrylamide gels.
GEO Accession
GSM3146456