GSM4421237: Naive CD4 T cell, resting; Homo sapiens; RNA-Seq
Total RNA was isolated from flow sorted naïve CD4+ T cells from two healthy donors using the RNeasy Plus Mini Kit from Qiagen. The TruSeq Stranded mRNA Sample Prep kit (Illumina, San Diego) was used for library preparation starting with 1-2 μg of RNA (R.I.N. >7) as input. The poly-A mRNA was fragmented 3 minutes at 94°C and every purification step was performed by using 1X Agencourt AMPure XP beads. Both RNA samples and final libraries were quantified using the Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA) and quality tested by Agilent 2100 Bioanalyzer RNA Nano assay (Agilent technologies, Santa Clara, CA). Libraries were processed with Illumina cBot for cluster generation on the flowcell and sequenced on single-end mode at the multiplexing level requested on HiSeq2500 (Illumina, San Diego, CA).
GEO Accession
GSM4421237