16S amplicon: Tomato rhizosphere
Tomato rhizosphere samples were collected from biochar amended and non-amended treatments. DNA were extracted and PCR amplification of 16S rRNA gene fragments was performed using 515F and 806R primers. PCR amplicon was sequenced using Illumina MiSeq technology.  Barcode and  primer sequences were trimmed, and data was analyzed using qiime pipeline version 1.9.1.