16S rRNA seq of activated sludge
Barcoded PCR primers 515F/806R (Caporaso et al. 2012) were used to partially amplify the 16S rRNA gene. Purified amplicons were pooled in equimolar quantities and sequenced on an Illumina MiSeq sequencer (Illumina, San Diego, CA), using pair-end 250 base pair kits, at the Cincinnati ChildrenŐs Hospital DNA core facility.