GSM2478801: TruSeq.Lab5.PlasmaPool; Homo sapiens; miRNA-Seq
Whole blood was collected from 11 healthy male participants, and was processed to separate and collect the plasma. Blood was collected in K2EDTA vacutainer tubes, inverted 8-10 times and centrifuged at 500xg for 10 minutes at room temperature. The plasma layer was then transfered to a fresh 15ml tube and centrifuged at 2500 xg for 10 minutes at room temperature. The top 80% of the plasma was transferred to a fresh 15ml tube. 5x volumes of QIAzol Lysis reagent was added to 1.2 ml of the plasma. RNA was extracted using the miRNeasy kit. Library construction protocols varied by sample (See lib.method.simple and lib.method.detail sample charateristics). TruSeq, CleanTag, NEBNext and 4N_NEXTflex protocols use the commercial kits and were optimized for sequencing of small RNAs from low-input biosamples. 4N_A, _B, _C and _D are in-house protocols using 5' and 3' adapters with 4N randomized terminal nucleotides.
GEO Accession
GSM2478801