GSM2395580: Day3_C16; Homo sapiens; RNA-Seq
We obtained EGFP (+)/DAPI (-) cells on day 3, and TRA-1-60 (+)/DAPI (-) cells on days 7-28 by sorting with FACS Aria II. RNA extraction and cDNA synthesis of single cells were performed using SMARTer Ultra Low Input RNA for Illumina Sequencing (Clontech) with the C1 Single-Cell Auto-prep and IFC for 10-17 µm (Fluidigm) according to the manufacturer’s instructions. Sequencing libraries were constructed using the Nextera XT DNA Sample Prep kit (Illumina) and Nextera XT Index kit (Illumina) according to Fluidigm’s instruction, which was modified from Illumina’s original protocol. The libraries were sequenced with the TruSeq SBS kit v3 and TruSeq SR Cluster kit v3 (Illumina) in 100-cycle Single-Read mode of HiSeq 2500 (Illumina).
GEO Accession
GSM2395580