transcriptome of 30
Total RNA was extracted using RNAplant Plus Reagent according to manufacturers instructions (Tiangen, Beijing, China). Before library construction, we evaluated the degradation of the RNA on a 1% agarose gel, and checked purity using the Qubit 3.5 Fluroer (Life Technologies, CA, USA), with integrity and concentration assessed using an RNA Nano 6000 Assay Kit on the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (#E7530L, NEB, USA) following the manufacturers recommendations and then sequenced on the Illumina HiSeq X Ten platform to generate 150 bp paired-end reads.