RNA-seq of Solanum tuberosum: breeding clone PW363 with Rysto, mock inoculated
Total RNA was isolated using RNeasy Plant Mini kit (Qiagen), treated with Dnase I (Invitrogen), cleaned using RNeasy MinElute Cleanup Kit (Qiagen), and rRNA was depleted using RiboMinus Plant kit. RNAseq library was prepared according to Applied Biosystems protocol: fragmentation by RNAse III (AB), adaptor hybridization and ligation, reverse transcription using ArrayScript RT (AB), cDNA purification using QIAGEN PCR Purification Kit, size-selection on 6% TBE-Urea gel (150-250 nt excised), cDNA amplification by PCR (15 cycles) and purification (PureLink Micro Kit), 6 full-scale ePCR reaction (AB ePCR kit).