GSM1933962: rpoS_EE_1; Escherichia coli str. K-12 substr. MG1655; RNA-Seq
RNA was extracted using TRIzol (Invitrogen), following the manufacturer's protocol. Total RNA was treated with DNase I (Invitrogen, Cat No. 18068-015) according to the manufacturer's protocol. Further precipitation of RNA and ribosomal RNA cleanup was achieved by Ambion MICROBExpress bacterial mRNA purification Kit (cat. no. AM1905) according to the manufacturer's protocol. The RNA was finally suspended in 10 μL RNAse free water. Libraries for Illumina HiSeq 1000 sequencing using the Truseq kit were prepared using protocols recommended by the manufacturer.
GEO Accession
GSM1933962