Raw reads from total genomic libraries of Melanophryniscus simplex sequenced as multiplex library in Illumina HiSeq 2000
Muscle tissue samples were stored in 70% ethanol at -20°C for several months/years. Materials were separately pooled for DNA extraction using the AGENCOURT® DNAdvance™ Genomic DNA Isolation Kit. Total genomic libraries were prepared using a NEBNext® DNA Library Prep Master Mix (NEb #E6040S) and subjected to NGS on a Illumina HiSeq 2000™ at the multiuser high-throughput sequencing facility of the Luiz de Queiroz College of Agriculture (ESALQ, USP, Piracicaba, Brazil).