GSM3604972: PIK3CA_H1047R_homozygous_cloneE [WTC11_E]; Homo sapiens; RNA-Seq
Cells were washed in DPBS and lysed with QIAzol. The RNA was extracted with the Direct-zol RNA Miniprep Kit (ZymoResearch # R2051) according to the manufacturer's instructions (eluted in 50 μl nuclease-free water). The quality of the RNA was confirmed on an Agilent 2100 Bioanalyzer using the RNA 6000 Nano Kit (Agilent # 5067-1511) according to the manufacturer's instructions, with all samples pre-diluted to 250-300 ng/μl in nuclease-free water. All samples had high RNA Integrity Number (RIN) scores of 9.9-10. A total of 1 μg RNA per sample was used as input to prepare indexed, 50-bp-long single-end mRNA libraries with Illumina's TruSeq Stranded mRNA Library Prep Kit (# 20020594) and TruSeq RNA Single Indexes Set A (# 20020492) according to the manufacturer's instructions. The integrity and quantity of the libraries were determined on the Agilent Bioanalyzer using the DNA 12000 Kit (Agilent # 5067-1508). mRNA sequencing of pooled libraries on an Illumina HiSeq 4000
GEO Accession
GSM3604972