GSM3145532: SCNT_Morula_replicate2; Bos taurus; RNA-Seq
Pools of 5 morula embryos were washed three times in RNase-free PBS solution and transferred into the lysis buffer immediately on ice. Samples were then centrifuged at 10000 rpm for 30 s at 4°C, and stored at -80°C. Five morula embryos for each sample were directly lysed and used for cDNA synthesis using SuperScript III (Thermo Fisher Scientific). To construct the cDNA library, cDNAs were amplified and fragmented with the NEBNext Ultra DNA Library Prep Kit (E7370, New England Biolabs). Paired-end 150 bp sequencing was performed on the HiSeq4000 (Illumina) platform.
GEO Accession
GSM3145532