RNA-seq of E.coli: OD>1.9
Libraries were constructed by designing PCR primers targeting the hokB gene with tails that match Illumina adapters (PCR1). A second PCR (PCR2) was carried to attach the adapters for the Illumina run. See: Dan Bar-Yaacov, Ernest Mordret, Ruth Towers, Tammy Biniashvili, Clara Soyris, Schraga Schwartz, Orna Dahan and Yitzhak Pilpel. RNA Editing in Bacteria Recodes Multiple Proteins and Regulates an Evolutionarily Conserved Toxin-Antitoxin System. Genome Research (2017). doi: 10.1101/gr.222760.117.constructed by designing PCR primers targeting the hokB gene with tails that match Illumina adapters (PCR1). A second PCR (PCR2) was carried to attach the adapters for the Illumina run