PipeDreams_VF16_36
"The extracted DNA (biofilms and water samples) was sent to the Research and Testing Laboratory (Lubbock, TX, US) for bacterial 16S rRNA gene tag-encoded FLX amplicon pyrosequencing (bTEFAP). PCR amplification was performed using the primers Gray28F and Gray519r (Callaway et al., 2010). Sequencing reactions utilized a Roche 454 FLX instrument (Roche, Indianapolis, IN) with Titanium reagents, titanium procedures, a one-step PCR reaction (35 cycles), and 1 U of HotStar Highfidelity Polymerase was added to each reaction (Qiagen, Valencia, CA)."