GSM4058861: N2C1; Caenorhabditis elegans; RNA-Seq
UV-irradiated or mock-treated L1 worms were washed off plates using M9 buffer, and suspended in TRIzol™ reagent (ThermoFisher) with 0.7mm zirconia/silica beads (Biospec Products), and homogenized with Precellys24 (Bertin).  After lysis, total RNA were purified using the RNeasy™ Mini kit (Qiagen) according to the manufacturers' specification, except for the use of  1-bromo-3-chloropropane (Sigma) instead of chloroform for phase separation. Each sample was run in triplicates. Around 100-150 ng/µl per sample was sent for sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM4058861