GSM4670420: Brain CD45+ cells, Cd300a-fl/fl, 1 hr after MCAO; Mus musculus; RNA-Seq
To isolate the brain cells, mice were sedated under deep anesthesia and perfused with PBS transcardially. Collected brain tissues were transferred to dounce homogenizer with 3.5ml of RPMI-1640 and grinded using loose and tight plunger. Homogenates were filtered through 70 micrometre cell strainer and then subjected to Percoll (GE Healthcare) gradient centrifugation. Cells were collected from the interlayer between 40% and 70% Percoll layers. Collected cells were filtered through 70 micrometre nylon mesh to obtain single-cell suspensions and PI-CD45+ hematopoietic cells were sorted by flow cytometry. 10X Genomics, Chromium Single Cell 3' Library and Gel Bead Kit v3.
GEO Accession
GSM4670420