WGS analysis of A04174
Fusobacterium genomic DNA was sheared with Covaris and PAGE-purified 200 bp and 450bp size fractions were polished, A-tailed, ligated to PE adapters and PCR amplified using indexed primers IDX2_000011 (200bp fraction = RH146_1 A04174) or IDX2_000012 (450 bp fraction = RH146_2 A04175) and PE primer 2.0. The amplicons were PAGE-purified, precipitated with Glycoblue (Ambion) carrier and resuspended in 17.0 ul EB (2 ul used for Nanodrop).