Full-Transcript sequencing of the C. remanei PX506 inbred line
Iso-Seq libraries of PX506 strain were prepared from with the Clonetech SMARTer PCR cDNA Synthesis kit for cDNA synthesis and PCR amplification with no size selection starting with 500ng of total RNA from a mixed staged population of C. remanei strain PX506 (Clonetech, Cat#634925).  PacBio library generation was performed on-site and sequenced on the PacBio Sequel I platform (GC3F, University of Oregon).