GSM1613468: fst2; Fusarium verticillioides; RNA-Seq
Total RNA was isolated from the content of each vial and purified with the RNeasy Mini Kit (Qiagen, Valencia, CA, USA).  Further purification was achieved by treatment with the DNA-Free RNA kit (Zymo Research, Irvine, CA, USA). The Purdue Genetic Core Facility conducted quality assessment, processing, and sequencing of the RNA.  The RNA samples had a RIN (RNA Integrity Number) over 7.0 as determined with an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA).  Paired-end sequences were obtained with an Illumina HiSeq 2500 sequencer (Illumina, San Diego, CA, USA).  Sequence data were trimmed of adapters and filtered to remove low quality sequence and reads less than 30 nt.
GEO Accession
GSM1613468