GSM4058224: A03_1; Bos taurus; RNA-Seq
RMECs were isolated from bovine retinas according to (Stitt, Chakravarthy, Archer, & Gardiner, 1995). RMECs were maintained in DMEM supplemented with 0.1mg/ml Primocin, 0.38μg/ml insulin, 1mg/ml heparin and 10% (v/v) porcine serum (PS). BRECs were passaged at approximately 80% confluency in a 1:3 ratio and grown until confluency at passage 3 for Fluidigm C1 runs . RMECs were dissociated form flask using TryPLE Express dissociation reagent and single cells were captured on Fluidigm C1 Microfluidics chip. Clontech modified Illumina® Nextera® XT DNA sample preparation protocol was used for single-cell mRNA sequencing library preparation from cDNA acquired using the C1 system which was miniaturised using the Labcyte Access Robotic System in conjunction with Echo 525 Acoustic Dispenser
GEO Accession
GSM4058224