GSM4057734: Emu_HH18_hindlimb_rep2; Dromaius novaehollandiae; ATAC-seq
Tissue was dissected from each embryo in cold PBS.  Tissue was immediately transferred to 1x Trypsin in EDTA Solution (Sigma) for 10-15 minutes at room temperature. Following trypsinization, the tissue was transferred to a neutralizing culture media (DMEM (Gibco) with 10% FBS (Gibco) and 1% Pen Strep (Gibco)) and pipette-mixed until homogenized. Homogenate was filtered through 35µm nylon mesh filters and filtrate was taken immediately to cell counting. 50,000 live cells were utilized for ATAC-seq tagmentation as described in Buenrostro et al. (2015).  Following tagmentation clean-up, 11 cycles of PCR were carried out and PCR products were cleaned using the Minelute PCR Purification Kit (QIAGEN).
GEO Accession
GSM4057734