Alternative splicing and trans-splicing events revealed by analysis of Bombyx mori transcriptome
Total RNAs from the 77 samples were isolated by Trizol (Invitrogen), and equally mixed together. The ribosomal RNAs were depleted by Ribominus kit (Invitrogen) under its protocol, then the RNA sample was fragmented by Covaris, and reverse transcribed by random primer followed by second-strand synthesis to create double-stranded cDNA fragments with blunt ends. These cDNA fragments were ligated with Illumina Single-End Sequencing adapters for PCR and sequenced by Illumina Genome Analyzer 2.