GSM4058386: Con3; Bos taurus; miRNA-Seq
The exoEasy Maxi Kit (no. 76064, QIAGEN) was used for the isolation of serum EVs. miRNeasy Serum/Plasma Kit (no. 217184, QIAGEN) was used to extract EV-miRNAs. TruSeq Small RNA Sample Prep Kits (no. RS-200-0012, Illumina), Qubit dsDNA Assay Kit (no. Q328520, Life Technologies), Qubit RNA Assay Kit (no. Q32852, Life Technologies), SuperScript II Reverse Transcriptase (no.18064-014, Invitrogen) and DNA loading dye (no.LC6678, Invitrogen) were utilized in this step, following the manufacturer's instructions. Briefly, 3' adapter and 5' adapter were ligated to the end of the RNA molecules. Then the libraries were reverse transcribed and PCR amplified subsequently. The cDNA constructs were purified and recovered, and the libraries was checked and normalized eventually.
GEO Accession
GSM4058386