Pot I Collection 1 Time 2 Hot Recovery
RNA isolated via TRI-reagent with cleanup on Qiagen Plant RNeasy. RNAs treated with DNaseI. 15 g of total RNA used in Illumina mRNA-seq Sample Preparation Kit. mRNA isolated and purified via Sera-Mag Magnetic Oligo(dT) Beads, washed, and fragmented using divalent cations under elevated temperature. cDNA templates purified by gel isolation for size selection of approximately 250 bp and amplified via PCR.