GSM1613115: P18 brain oligodendrocytes_ERRBS_n3; Mus musculus; Bisulfite-Seq
Genomic DNAs were obtained from FAC-sorted P2 oligodendrocyte progenitor cells or P18 oligodendrocytes, isolated from mouse brains. We used AllPrep DNA/RNA Mini Kit (Qiagen), in accordance with the manufacturer’s protocol, to isolate DNA and RNA simultaneously from the same cell pellet. DNA purity was assessed by measuring the A260/A280 ratio using a NanoDrop (Thermo Scientific), and DNA quality checked by agarose gel electrophoresis for a single high molecular weight band. ERRBS libraries for DNA methylation analysis were prepared from 50 ng input DNA per biological replicate following a modification (Akalin et al., 2012a) of a published protocol (Gu et al., 2011) .
GEO Accession
GSM1613115