WSSV-resistant Penaeus monodon microRNAs using the Illumina HiSeq 2500 with a single-end sequencing length of 50 bp
Total RNA was prepared from pleopod tissues of naturally occurring  WSSV-resistant shrimp samples using RNeasy Mini Kit. Library of isolated total RNA was performed according to the protocol of Illumina TruSeq Small RNA Sample Preparation. PCR amplified products were gel eluted and purified products were sequenced on the Illumina HiSeq 2500 according to the manufactures recommended protocol.