RNA-Seq of Chloridea subflexa: adult male and female thorax
Adult Chloridea subflexa were sacrificed by freezing at -80C and subsequently dissected just prior to RNA isolation. 1/2 of a thorax was used for extraction. Male and female thoraces were extracted separately, and the extracted RNA was subsequently combined for sequencing. RNA was isolated according to Trizol protocol, then processed on RNEasy columns for clean up. RNA was quantified using Bioanalyzer and Qubit, the sent to the NY Genome Center for library preparation and sequencing. Libraries were prepared using the Ovation RNA-Seq System for preparing amplified cDNA from total RNA followed by Illumina TruSeq Nano DNA Sample Preparation. 125bp paired-end sequencing was performed on an Illumina HiSeq 2500.