GSM1611875: WT rep1; Mus musculus; RNA-Seq
Total RNA was prepared (Qiagen miRNeasy), digested with DNase 1 and RNA quality was assessed by capilliary electrophoresis (Agilent 2100 Bioanalyzer). A directional transcriptome RNA-seq protocol was employed. This included RiboZero treatment of the RNA, first strand synthesis primed with random hexamers and oligo(dT), second strand synthesis in the presence of dUTP, fragmentation, end repair, dA tailing, ligation of Illumina indexed adapters, UNG treatment and PCR amplification (18 cycles).
GEO Accession
GSM1611875