GSM3901208: control-1(sham); Rattus norvegicus; ncRNA-Seq
At 1day post-surgery, rats in all groups were euthanized with an overdose of 2% 2% Sodium pentobarbital solution, and a 1-cm long segment of spinal cord, including the injury epicenter, was quickly dissected and collected without transcardial perfusion in advance and was then fresh frozen in liquid nitrogen.sequencing libraries are size-selected for the RNA biotypes to be sequenced using an automated gel cutter.  Sequencing library was determined by Agilent 2100 Bioanalyzer using the Agilent DNA 1000 chip kit (Agilent, part # 5067-1504). The libraries were denatured and diluted to a loading volume of 1.3ml and loading concentration of 1.8pM. Diluted libraries were loaded onto reagent cartridge and forwarded to sequencing run on Illumina NextSeq 500 system using NextSeq 500/550 V2 kit (#FC-404-2005, Illumina), according to the manufacturer's instructions
GEO Accession
GSM3901208