Heliothis virescens transcriptional response to the nematode-bacterial infection
Total RNA was extracted from fourth instar larvae Heliothis virescens at different time points post infection with nematode Heterorahbditis bacteriophora.  Total RNA was used to isolated mRNA using biotin labeled Oligo d(T) and magnetic beads. The first strans cDNA was synthesized from mRNA using random primers followed by RNaseH digestion and the second strand synthesis. Double-stranded cDNA was sheared, end repaired, and dA tailed prior to ligating Illumina TruSeq indexed adapters. The sequencing was performed on an Illumina MiSeq instrument.