Transcriptional and epigenetic profiling of the progression of hESCs to beta cells
Transcriptional and epigenetic profiling of the progression of hESCs to beta cells
All ChIP-seq libraries were prepared as per Illuminas instructions (http://www.illumina.com). Briefly, total ChIP DNA was subjected to end-repair and ligated to the Adaptor Oligo Mix (Illumina). For input library preparation, 10 ng of input DNA from each sample was used. After adaptor ligation, DNA fragments were size-fractionated by gel electrophoresis and excised at (+25 bp,-25bp). Following gel purification, DNA fragments were amplified with 18 PCR cycles and purified using a MiniElute PCR Purification kit (Qiagen).
Experimental Factor: TIMEPOINT
day 5
Experimental Factor: CD antibody
n/a
Experimental Factor: histone antibody
input
Experimental Factor: CELL_TYPE
embryonic stem cell
Experimental Factor: CELL PROPERTY
embryonic stem cell directed to primitive gut tube