GSM3901319: Control 5 days, biological rep2; Pinus elliottii; RNA-Seq
Total RNA of the sixteen libraries was extracted from vascular cambium of slash pine plants using Concert plant RNA reagent157 (Life Technologies). Nanodrop spectrophotometer (Thermo Scientific) was used to quantify RNA concentration. Samples were precipitated with 3 M sodium acetate, 5 mg.ml-1 glycogen and ethanol. Pellets were kept in 70 % ethanol for shipping. Library preparation and RNA sequencing were performed with Fasteris S.A. standard protocols (single-end 1x 125 bp Illumina HiSeq High- Output) after depletion of rRNA with RiboZero kit.
GEO Accession
GSM3901319