Channel catfish Testis RNA-Seq
RNA-seq library preparation and sequencing was carried out by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA). cDNA library was prepared with ~4 ug of starting total RNA following the protocols of the Illumina TruSeq RNA Sample Preparation Kit (Illumina). The library was amplified with 15 cycles of PCR. The final library had an average fragment size of ~270 bp and final yields of ~400 ng. After KAPA quantitation and dilution, the library was sequenced for one lane on an Illumina HiSeq 2000 instrument with 100 bp paired end (PE) reads.