RNA-seq of tea leaves-control
mRNA was purified from 3 g of total RNA using poly-T oligo-attached magnetic beads and used to generate library for RNA sequencing using NEBNextUltraTM RNA Library Prep Kit for Illumina(NEB, USA) following the manufacturers recommendations. The library quality was assessed on the Agilent Bioanalyzer 2100 system. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumia) according to the manufacturers instructions. After cluster generation, the library preparations were sequenced on an Illumina HiSeq2500 platform and 125 bp/150 bp paired-end reads were generated