GSM4059688: CLIB214 CSE4-3HA R1 IP; Candida parapsilosis; ChIP-Seq
Immunoprecipitation was performed with EZview Red Anti-HA Affinity Gel (Sigma–Aldrich) or mouse IgG1 (Cell Signaling Technology) as isotype control. After overnight incubation with the selected antibody, bound DNA was washed with FA lysis buffer containing 1mM PMSF and eluted with HA peptide (Sigma–Aldrich) by incubation for 30 minutes at 30C. Following addition of Pronase (Sigma–Aldrich) and ChIP elution buffer (50 mM Tris-HCl, pH 7.5, 10 mM EDTA, 1% w/v SDS) to the HA peptide elution supernatant, crosslinks were reversed, and the samples were purified by phenol–chloroform extraction. BGISEQ500 ChIP-seq library construction carried by Beijing Genomics Institute (http://www.genomics.cn/en/index).
GEO Accession
GSM4059688