GSM2396727: ecoli_k12_30C_m_2; Escherichia coli str. K-12 substr. MG1655; RNA-Seq
Cells were lysed in liquid nitrogen, then samples were taken for total RNA. Total RNA was extracted with phenol chloroform and alkaline fragmented, then fragments (20-40 nt) were gel-purified. RNA was dephosphorylated, ligated to an adaptor, reverse transcribed, circularized then amplified with Illumina adaptors and barcodes.
GEO Accession
GSM2396727