GSM1937526: ara-4h; Pyricularia oryzae; RNA-Seq
Total RNA was extracted from mycelium ground in a Tissue Lyser II (Qiagen) using TRIzol reagent (Invitrogen) according to the instructions of the manufacturer. Total RNA samples were purified with NucleoSpin RNA Clean-up Kit (Macherey-Nagel, Germany). Contaminating DNA is removed by an rDNase solution directly on the silica membrane. RNA quality was checked with the RNA 6000 Nano LabChip Kit on a 2100 Bioanalyzer (Agilent Technologies). RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM1937526