GSM1932958: tra, esg>traF female rep1; Drosophila melanogaster; RNA-Seq
Midguts were dissected (x30 by replicate), and RNA were extracted using Trizol reagent. Illumina Truseq mRNA stranded library prep kit was used with 500ng of total RNA for the construction of sequencing libraries RNA libraries were prepared for sequencing using standard Illumina protocols Library quality was checked on a Bioanalyser HS DNA chip and concentrations were estimated by Qubit measurement. Libraries were pooled in equimolar quantities and sequenced on a Hiseq2500 using paired end 100bp reads
GEO Accession
GSM1932958