Genome sequencing of the Arcanobacter lacustris SAG
The single cell was obtained with fluorescent activated cell sorting (FACS) of the sample and subsequently lysed. Extracted DNA was subjected to real time multiple displacement amplification (rtMDA). The MDA product was then verified by SSU rRNA qPCR. A second round of MDA was required to obtain sufficient quantity of genomic DNA for Illumina sequencing. All above steps were carried out at the Bigelow Laboratory Single Cell Genomics Centre (Maine, USA). A short-insert paired-end TruSeq library (Illumina) was prepared according to the manufacturer’s prescriptions.