GSM2825368: Endothelium Kitl null 2; Mus musculus; RNA-Seq
E11.5 AGM was manually dissected and prepared as single cell suspensions using a Collagenase I-based digestion, then hematopoietic clusters (Ter119- VE-Cadherin+ c-Kit+) were FACS-sorted into lysis buffer (1:20 Rnase inhibitor, 0.2% Triton X-100). RNA-Seq libraries prepared using Smart-seq2 protocol (Picelli et al., Nature Protocols 2014)
GEO Accession
GSM2825368