GSM1611763: GRHL2_ChIPSeq; Mus musculus; ChIP-Seq
Twenty-five C57BL/6 E17.5 mouse placentas were harvested, maternal uterus tissue was removed and placentas were pooled. Cross-linking was performed with 1% formaldehyde in PBS for 15 min at room temperature. Chromatin was fragmented to an average size of 300-500 bp by sonication. 100 μg chromatin and 5 μg GRHL2 antibody or isotype control antibody (rabbit IgG) as background control were used per assay using a Chromatin Immunoprecipitation Assay Kit (Upstate, Millipore) according to the manufacturer’s protocol. Libraries of 250-350 bp were prepared for sequencing according to standard Illumina's instructions.
GEO Accession
GSM1611763