GSM1611872: vector_ChIP-seq; Homo sapiens; ChIP-Seq
Lysates were clarified from sonicated nuclei and the FLAG fusion Brd7 protein-DNA complexes were isolated with mouse-anti-human, Monoclonal ANTI-FLAG® M2 antibody (Sigma Aldrich, Cat# F1804) Illumina Paired-End DNA Sample Prep kit (Part#1005063) was used to perform end repair, adenylate 3' ends and to ligate adapters.The purified adapter-ligated DNA fragments were then PCR amplified and the products with length of 100bp - 300bp(including adapters) were used for library construction.
GEO Accession
GSM1611872