GSM1933254: nucleosome core particles (+SM) rep 1; BY4741; Saccharomyces cerevisiae; MNase-Seq
In ChIP-seq experiments, cells were cross-linked with formaldehyde before harvesting. In MNase-seq experiments, nuclei were prepared from unfixed yeast cells and then digested with MNase. Mono-nucleosomal DNA was gel-purified (~150 bp). See paper for details. The DNA was repaired using the DNA repair kit from New England Biolabs. The repaired DNA was processed for paired-end sequencing according to the Illumina protocol.
GEO Accession
GSM1933254