GSM4670327: C6-29 Post1; Homo sapiens; RNA-Seq
Cryopreserved PBMCs of the AML patients were quickly thawed and carefully transferred to the 35ml of pre-warmed α-MEM media (22 °C). Cells were pelleted by centrifugation at 300xg for 5 min at 22 °C, and then washed with 30ml of 0.04% BSA in PBS (22 °C). Live cells were collected by sorting with FACS after staining with CD45 and PI. RNA libraries were prepared for sequencing using Single Cell 3' Reagent Kit v3 (10x genomics).
GEO Accession
GSM4670327