GSM4059065: Vehicle_Light-polysome_replicate-1; Mus musculus; RNA-Seq
The RNA extraction was performed using TRIzol reagent (ThermoFisher) following instructions in the manual. For the cell samples, total RNA was extracted using regular TRIzol reagent (1 ml for a plate of cells) and placed at room temperatur for 5 mins to lyse the cells. For the polysome profiling fraction samples, RNA extraction was performed using TRIzol LS reagent at a volume ratio of 1:3 following the manual. Illumina TruSeq Stranded mRNA
GEO Accession
GSM4059065