RNA-seq of unopened flower buds of Crotalaria juncea cv SUN-053
Paired-end cDNA sequencing libraries was prepared from the total RNA from pooled unopened flowerbuds of C. juncea cv SUN-053 using TruSeq Stranded mRNA Library Prep Kit of Illumina as per the manufacturer's instruction. Briefly, rRNA was depleted from the total RNA followed by fragmentation. The fragmented, rRNA depleted RNA, was converted into first-strand cDNA, followed by second-strand cDNA generation, A-tailing, adapter ligation and finally ended by index PCR amplification of adaptor-ligated library. The amplified libraries were analyzed in Bioanalyzer 2100 (Agilent Technologies) using High Sensitivity (HS) DNA chip as per manufacturer's instructions. Finally, the sequencing library was loaded into Illumina NextSeq 500 platform for cluster generation and sequencing through Paired-End RNA sequencing (in both the forward and reverse directions: 2 x 150 bp) approach.