Illumina HiSeq 2500 sequencing; SOX7 ChIP-seq in HUVECs
SOX7 ChIP-seq in HUVECs
HUVECs were obtained from LONZA and grown according to suppliers recommendations. Cells were cultured in EBM2 media, supplemented with EGM2. Transfection was performed using Roche X-tremegene DNA transfection reagent in low (0.4%) FBS  Following IP, DNA amplification was performed using TruSeq ChIPseq kit (Illumina, IP-202-1012). The PCR product was purified using AMPure beads. The library was quantified using the KAPA library quantification kit for Illumina sequencing platforms (KK4824)
Experimental Factor: anti-RCFP (Clontech, #632496)
immunoprecipitate
Experimental Factor: transfected with mCherry
genotype