16S rRNA amplicons from Cornopsylla rotundiconis: male
Genomic DNA was extracted from whole individual psyllids using QiaAmp Mini DNA Extraction kit (Qiagen) including RNase treatment. Amplification of the 16S rRNA gene was performed using primers 341F (5' CCTACGGGNGGCWGCAG 3') and 805R (5' GACTACHVGGGTATCTAATCC 3'), which encompass the V3-V4 region and produce a fragment of approximately 464bp, including primers. Library preparation, inputting 7ng of DNA extract, was utilised the Nextera XT kit, and 2 x 300bp paired end fragments sequenced on a 384-multiplexed Illumina MiSeq run.