Illumina HiSeq 2000 sequencing; Identification of the FRS12 regulated genes by RNA-Seq Analysis in Arabidopsis thaliana
Identification of the FRS12 regulated genes by RNA-Seq Analysis in Arabidopsis thaliana
RNeasy Plant Mini Kit (Qiagen) and DNase I treated (Promega) Preparation of six strand-specific cDNA libraries: Purification of poly-A containing mRNA molecules; mRNA fragmentation; Random primed cDNA synthesis (strand specific); Adapter ligation and adapter specific PCR amplification
Experimental Factor: genotype
GFP-GR
LIBRARY_STRAND
first strand