GSM2478560: wild-type (Col-0); Arabidopsis thaliana; RNA-Seq
Globular to heart stage lefko2 mutant and wild-type Arabidopsis embryos were dissected from siliques. Total RNA was extracted using the NucleoSpin Plant RNA kit (Macherey-Nagel, Düren, Germany) with an on-column DNase treatment according to the manufacturer's instructions. The quantity and integrity of the RNA were assessed using a NanoDrop 1000 spectrophotometer and agarose gel electrophoresis. RNA-seq libraries were generated using the TruSeq Low Input kit according to the manufacturer's instructions (Illumina).
GEO Accession
GSM2478560