HaCaT_C2-p63_H129-ChIPseq-1
25 million HaCaT C2 (single cell clone of HaCaT parental cell line) cells were crosslinked with formaldehyde (1.5%) for 10 minutes at room temperature, collected by scraping, and sonicated to yield ~300 bp DNA fragments using a Diagenode Bioruptor. ChIP was performed using 10 g of p63 H-129 (Santa Cruz Biotechnology, sc-8344) antibody. Input control samples were collected from sonicated samples prior to performing ChIP. ChIP-seq libraries were prepared as described previously in Marshall et al., Cell Reports, 2016. Illumina sequencing of ChIP-seq libraries was performed at the Vanderbilt VANTAGE core using a single-end 75 bp protocol.