ER4
For Illumina sequencing, 2 g of purified pooled total RNA from three replicates was used for the preparation of strand-specific RNAseq libraries with 14 cycles of final amplification (Zhong et al 2012). The purified libraries were multiplexed and sequenced with 101 bp paired-end read length in two-lanes on an Illumina HiSeq2500 instrument (Illumina, San Diego, CA) at the Cornell University Biotech Facility (Ithaca, NY).