GSM4057289: MeDIP-seq of DNA from pig-525; Sus scrofa; MeDIP-Seq
DNA was extracted from the mature adipocytes using the Epicenter kit (Illumina), and total RNA was isolated from the mature adipocytes according to the protocol described in (Cirera, 2013) using TriReagent and included a DNAse treatment step. RNA libraries were prepared for sequencing using Truseq lib prep kit from Illumina together with RiboZero Gold  using 0.5ug of total RNA as input. mRRBS libraries were prepared as described in Boyle (2012) using 200ng DNA as input. MeDiP libraries were prepared from 1.1ug DNA, fragmented in a bioruptor and methylated DNA fragments were enriched using MethylMiner Methylated DNA Enrichment KIt (cat#ME10025). Sequencing libraries were generated according to the low Input TruSeq library preparation protocol.
GEO Accession
GSM4057289