Whole genome sequence of sequentially passaged NTHI
Genomic DNA was purified using a Gentra Puregene Yeast/Bact. Kit (QIAGEN, Germantown, MD). Sequencing ibraries were then prepared and sequenced using paired end 300bp chemistry on the MiSeq platform (Illumina, San Diego, CA). Nucleotide variants were identified using the Churchill algorithm and the percentage of reads that differ from the reference genome was calculated. Nucleotide variants were validated by Sanger sequencing.