GSM4120812: foxo_Starv_rep1; Drosophila melanogaster; RNA-Seq
Eye imaginal discs were dissected in HBSS and mounted in a 0.5 µL drop of HBSS on FrameSlide PET (Carl Zeiss AS). Single clones of approximately 50 cells were microdissected using a Zeiss PALM-Microdissection system and captured into the caps of AdhesiveCap (Carl Zeiss AS) microcentrifuge tubes. The samples were frozen in liquid nitrogen and stored at -80°C until RNA isolation. RNA isolation and cDNA library preparation from the microdissected samples were done according to the Ovation SoLo RNA-seq Systems kit (NuGEN Cat#0502-32).
GEO Accession
GSM4120812