Microbial diversity of SBR activated sludge at a low temperature
V4 region of 16S rRNA gene was amplified with primers 515F(GTGCCAGCMGCCGCGGTAA) and 806R (GGACTACHVGGGTWTCTAAT) under following PCR programs: initial denaturation at 95 буC for 2 min, followed by 30 cycles at 95 буC for 30 s, 53 буC for 20 s and 72 буC for 60 s, and followed by final extension at 72 буC for 5 min.