GSM4059333: at-microspore.sRNA.nrpd1a-3.replicate2; Arabidopsis thaliana; ncRNA-Seq
Total RNA was treated with DNAseI (Thermofischer: cat-EN0521) and then loaded on a 15% TBE-Urea polyacrylamide gel. RNA with a size of 15-27-nt was retrieved and eluted by crushing the gel in PAGE elution buffer (1M Tris pH7.5, 2.5M NaOAc, 0.5M EDTA pH8) followed by an overnight incubation and a new TRizoL extraction. sRNA seq libraries were generated with the NEBNext® Multiplex Small RNA kit (NEB #E7300S).
GEO Accession
GSM4059333