Ion Torrent Proton sequencing; RNA-seq of human testicular germ cell cancer
RNA-seq of human testicular germ cell cancer
Use of tissue samples remaining after diagnosis for scientific reasons was approved by Medical Ethical Committee (MEC) of the Erasmus MC Rotterdam (The Netherlands), permission 02.981. This included the permission to use the secondary tissue without further consent. Samples were used according to the 'Code for Proper Secondary Use of Human Tissue in The Netherlands' developed by the Dutch Federation of Medical Scientific Societies (FMWV (Version 2002, update 2011)). Tissues were stored frozen in liquid nitrogen or as formalin-fixed and paraffin-embedded (FFPE) blocs. RNA was extracted from frozen tissue samples using TRIZOL and purified on RNeasy column (Qiagen) QC was performed on Agilent Bioanalzer using a Eukaryote total RNA Pico chip, vs 2.6. RNA samples were rRNA reduced with RiboZero Gold and library preparation was performed using the using the Ion Total RNA-seq kit
Experimental Factor: individual
T6107