GSM2396926: poolH_mouse2; synthetic construct; ncRNA-Seq
Library preparation was performed as previously described (Knott (2014) Mol Cell). In brief, genomic DNA was extracted from the pre-injection pool and the bone marrow cell suspensions using the QIAamp Blood DNA Maxi Kit (Qiagen). For each sample, shRNA hairpins were extracted from genomic DNA in 96 separate 25-cycle PCR reactions where 2 mg of input DNA was included in each reaction. Following this initial PCR, Illumina adapters were added via PCR, and samples were processed on the Illumina MiSeq platform.
GEO Accession
GSM2396926