hMeDIP-Seq of Eriocheir sinensis: developing testis1
Male crabs at immature stage of testes were purchased from an aquaculture farm in Ganyu County, Jiangsu Province, China. Purified testis genomic DNA was sonicated into short fragments with a size range of 100-500 bp by a Bioruptor. The sheared DNA was then subjected to enrich 5-hmC-containing DNA using the 5-hydroxymethylcytidine antibody. The resulting enriched 5-hmC DNA was then subjected to sequencing library construction. The adapter-ligated 5-hmC library was amplified by PCR.