Time.0.Sediment.Pond.2
Water samples filtered through 0.1 micron filters and surface sand collected in 2 mL tube. DNA extraction on environmental sample using MoBio PowerSoil kit, 2 rounds of PCR with a clean up using magnetic beads following each round. PCR product was visualized using PAGE after the second PCR. The final cleaned PCR products were then pooled and submitted for sequencing on the Illumina MiSeq.