GSM4670365: 14_S13: Infected 72h replicate 1; Eimeria tenella; Gallus gallus; RNA-Seq
For RNA isolation 1 ml of HD11 cell lysate in TRIzol was used and RNA was extracted using chloroform according to the TRIzol manufacturer's protocol. The Isolated RNA was subsequently treated with DNAse (TURBO™ DNase, 2U/ul, ThermoFisher Scientific) according to the manufacturers protocol and further purified using reagents and the “RNA cleanup” protocol of the RNeasy Mini kit (Qiagen) protocol. RNA concentration and quality was then assessed using the Agilent RNA 6000 Nano kit on a 2100 Bioanalyzer Instrument (Agilent) and the RNA stored at -70°C until further analysis. Sequencing libraries were prepared from 0.12 – 0.5 μg total RNA using the TruSeq stranded mRNA library preparation kit (Cat# RS-122-2101/2102, Illumina Inc.) including polyA selection. The library preparation was performed according to the manufacturers' protocol (#15031047). Sequencing: HiSeq2500, paired-end 125bp read length, v4 sequencing chemistry.
GEO Accession
GSM4670365