RNA-Seq of manganox enrichment: 77% Mn oxidized
Sample preserved using Lifeguard Solution (Qiagen) and dissolved using DCBE solution. RNA extracted using RNeasy Mini Kit (Qiagen) and purified using CleanAll DNA/RNA Clean-up and Concentration Micro Kit (Norgen Biotek). DNA was removed using DNase Max Kit (Qiagen). Library constructed using NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs).