GSM2396728: ecoli_k12_30C_f_2; Escherichia coli str. K-12 substr. MG1655; OTHER
Cells were lysed in liquid nitrogen, then samples were taken for ribosome footprint extraction. Polysomes were digested with MNase, monosomes were purified by sucrose gradient ultracentrifugation, and the resulting mRNA fragments were extracted with phenol chloroform and gel-purified. RNA was dephosphorylated, ligated to an adaptor, reverse transcribed, circularized then amplified with Illumina adaptors and barcodes.
GEO Accession
GSM2396728