Sequencing of Barley chromosome 3 long arm within the GABI-BARLEX project
Individual barley chromosomes and chromosome arms were isolated by flow sorting and shotgun sequenced by 454 Titanium pyrosequencing.
Intact mitotic chromosomes were isolated by flow cytometric sorting and the purity of the obtained chromosome suspension was determined by FISH essentially as described previously (Suchankova et al., 2006). The DNA of sorted chromosomes was purified and amplified by MDA as described by Simkova et al. (2008).