RNA-seq of mus musculus: stomach organoid
Total RNA was extracted from gene KO organoids by the RNA extraction kit. Quality of total RNA was assessed by Bioanalyzer (Agilent) to ensure that RIN (RNA integrity number) was above 7.0. Double-stranded cDNA libraries (RNA-seq libraries) were prepared using SMARTer Stranded Total RNA-Seq Kit v2  Pico Input Mammalian (Clontech) according to the manufacturers instruction.