RNA-seq of Mimachlamys varia adult male, multiple tissues
Total RNA was isolated from five distinct tissues collected from this individual (digestive gland, mantle, gills, adductor muscle and gonads), using 50 mg of each tissue type. RNA extractions were performed using the Nucleospin RNA Set for Nucleozol kit (Macherey-Nagel). After determining RNA concentration using a Nanodrop 2000 spectrophotometer (Thermo Scientific), the five RNA extractions were pooled in equal amounts (4  g of RNA per tissue type). The quality of the RNA pool was assessed on an Agilent Bioanalyzer before normalized random primed cDNA library preparation.