GSM3146170: MH_S_Lcor_1; Mus musculus; Lichtheimia corymbifera; RNA-Seq
Fungal RNA was isolated using RiboPure Yeast Kit (Thermo Fisher) and macrophage RNA was isolated with the RNeasy Mini kit (Qiagen), according to the manufacturers' instructions. The concentration and purity of RNA samples were assessed using a NanoDrop spectrophotometer and Agilent 2100 bioanalyzer. Pooling ratio: L. corymbifera : MH-S = 1 : 3.9 Random-primed cDNA library per samples, Isolation of poly(A)+ RNA mRNA-Fragmentation, Random-primed cDNA synthesis adapter-ligation and PCR amplification Illumina HiSeq 2500, Sequence Mode: HSHOv4 SR50
GEO Accession
GSM3146170