GSM2563998: AR1-/AR2- rep 1; Escherichia coli; RNA-Seq
Cell pellets were lysed in Tissue and Cell lysis solution (EpiCentre) and proteinase K. RNA was isolated and DNase treated using the RNeasy Kit (Qiagen) using the manufacturer’s protocol. The amount of total RNA in each sample was quantified using the Qubit 2.0 Flurometer (Life Technologies) and quality was assessed using the RNA6000 Nano Chip on the Bioanalyzer 2100 (Agilent). rRNA depletion, fragmentation, reverse transcription, tagging, barcoding, limited cycle PCR (Illumina) ScriptSeq v2 Complete Kit (EpiCentre)
GEO Accession
GSM2563998