GSM1696273: Control_L1_C; Caenorhabditis elegans; RNA-Seq
Worms were pelleted into TRIzol.  Total RNA was extracted following the manufacturer's instructions.  Poly A-enriched RNA was isolated using the Qiagen OligoTex kit. 3’-end enriched RNA-sequencing (“3SEQ”) libraries were prepared as previously described (Van Nostrand and Kim, 2013, Beck et al., 2010). Briefly, barcoded sequencing adapters were ligated onto cDNA made from poly-A-enriched RNA. Libraries were pooled prior to sequencing.
GEO Accession
GSM1696273