RNA-Seq of Sus scrofa: juvenile female blood
Total RNA from sorted TCR-gd CD2+ cells of a four-month-old conventional pig was reverse-transcribed into cDNA using the SMART technology and oligo(dT) primers (SMARTer PCR cDNA synthesis kit). Following reverse transcription, the cDNA population was enriched for full-length V(D)J TCR transcripts with pTRGC primers including Illumina adaptor sequences as overhangs for Illumina MiSeq analysis.