Se-uninfected-1
We isolated head tissue from for three biological replicates each of Microphallus-infected vs. Potamopyrgus antipodarum collected from each of 3 New Zealand lakes: Alexandrina, Kaniere and Selfe (18 total samples). Head tissue from 7 individuals was pooled for each biological replicate and RNA was extracted using TRIzol. RNA quantity and quality were assessed with a Bio-Rad Experion Automated Electrophoresis Station, following manufacturer protocol, requiring an RQI >/= 8 and a minmum of 2ug of total RNA. We generated cDNA libraries following the Illumina TruSeq LS protocol, and then performed 2x100bp paired-end RNA sequencing on an Illumina HiSeq 2000. Libraries were split in half and sequenced two separate lanes (2 technical replicates per biological replicate).