GSM2824331: FF6mo1; Mus musculus; RNA-Seq
Tissue samples or cell lines were homogenized in Trizol and total RNA was extracted using Trizol and isopropanol precipitation. Poly(A)+ mRNA was then isolated from each total RNA sample using streptavidin-coated superparamagnetic beads (Dynabeads, Life Technologies) coated in biotinylated LNA-oligo(dT) (Exiqon). Purified poly(A)+ mRNA from each sample was reverse transcribed and Illumina sequencing libraries were enriched usign the SMARTer Stranded RNA-Seq kit (Clontech). The resulting libraries were sequenced on an Illumina HiSeq 2500 or NextSeq 500 with single-end 100-base or 75-base reads, respectively. template-switching RNA-Seq with random primers
GEO Accession
GSM2824331