GSM2395546: PCDH7-2; Homo sapiens; RNA-Seq
Total RNA was purified from HBEC-shp53-PCDH7 or control HBEC-shp53-GFP cells using RNeasy Mini Kit (Qiagen). Four micrograms of total DNase-treated RNA were prepared with the TruSeq Stranded Total RNA LT Sample Prep Kit from Illumina. Poly-A RNA is purified and fragmented before strand specific cDNA synthesis. cDNA were A-tailed and indexed adapters were ligated. Samples were PCR amplified and purified with AmpureXP beads and validated on the Agilent 2100 Bioanalyzer. Before being normalized and pooled, samples were quantified by Qubit (Invitrogen). Sequencing was performed on an Illumina HiSeq 2500 to generate 51-bp single-end reads.
GEO Accession
GSM2395546