GSM3683333: Treg depleted [Sample_95619]; Mus musculus; RNA-Seq
Cell lysates of FACS sorted cells were homogenized using QIAshredder, then total RNA samples were isolated using RNeasy Plus Micro Kit (Qiagen), including an on-column DNase treatment using RNase-Free DNase Set (Qiagen) according to manufacturer's instruction. RNA concentration and quality was determined and ribo depleted strand mRNA libraries were prepared by the University of Michigan Sequencing Core using SmartER-Pico kit. Libraries were multiplexed (7 per lane) and sequenced using paired end 50 cycle reads on a HiSeq 4000 (Illumina).
GEO Accession
GSM3683333