GSM1614233: Bovine_cumulus_GV_b; Bos taurus; ncRNA-Seq
RNA was extracted using TRIzol (Ambion) according to manufacturer’s instruction. NaIO4 treatment was performed on RNA enriched for small RNA (mirVana, Ambion). For oxidation, RNA was exposed to 200mM NaIO4­ ­in borate buffer (29.6mM borax, 29.6mM boric acid, pH6.8) for 10’/RT, then the reaction was stopped by the addition of glycerol. After precipitation with isopropanol and a 75% ethanol wash, libraries were prepared. Library preparation was based on the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England BioLabs) with modifications. The 3’ and 5' adapters contained 4 random bases at the 5’- and 3’-end, respectively, and were chemically synthesized by BiooScientific.
GEO Accession
GSM1614233