GSM1888227: HepG2 genomic rep1; Homo sapiens; OTHER
Next generation sequencing library preparation was performed as previously described (Kwasnieski et al. 2012; White et al. 2013; Kwasnieski et al. 2014).  Nucleic acids were purified from cell lysates using Norgen Total RNA Purification Kit (Norgen Biotek Corporation) and DNeasy Blood and Tissue Kit (Qiagen) for RNA and DNA, respectively.  RNA was treated with DNaseI (Turbo DNase, Life Technologies) and reverse transcription was performed using Superscript II reverse transcriptase (Life Technologies).  The 3’ UTR of the luciferase gene containing barcode sequences was amplified (Phusion high fidelity master mix, New England Biolabs) from cellular RNA (cDNA) or DNA prior to next-generation sequencing.  Each replicate experiment was sequenced as a pool on one lane on an Illumina HiSeq 2000 sequencer. Illumina Single End
GEO Accession
GSM1888227