Illumina HiSeq 1500 sequencing; The transcriptional signature of ascites-associated macrophages is linked to interferon signaling and a favorable clinical outcome in a subgroup of ovarian carcinoma patients
The transcriptional signature of ascites-associated macrophages is linked to interferon signaling and a favorable clinical outcome in a subgroup of ovarian carcinoma patients
Ascites was collected from patients with high-grade serous ovarian carcinoma undergoing primary surgery at the University Hospital in Marburg. Informed consent was obtained from all patients according to the protocols approved by the local ethical committe. Cells were extract by CD14 MACS. After Ficoll gradient centrifugation, TAMs were purified by CD14 MACS and lysed in Peqgold (TRIzol-like solution based on phenol and chloroform). RNA was digested with DNase (MN) and purified on a Qiagen Minelute column. Illumina-compatible libraries were made using the Epicentre Scriptseq human/mouse/rat low input Gold kit. Libraries were created using the Illumina Truseq mRNA kit v2 used according to the manufacturer's instructions.
Experimental Factor: 91
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