GSM2901316: Nab_ip_15_2; Saccharomyces cerevisiae; RNA-Seq
RNA was isolated using hot acid phenol method. For 4tU IP, 4tU residues in total RNA were biotinylated using HPDP-biotin; free HPDP-biotin removed and biotin-labeled RNA then purified on streptavidin beads, eluted from the column using beta-mercaptoethanol and RNA purified. Libraries were prepared using Lexogen QuantSeq REV kit (Lexogen GmbH, Vienna, Austria).
GEO Accession
GSM2901316