GSM4057519: HL-60_Dnase; Homo sapiens; DNase-Hypersensitivity
Cells were resuspended in RSB buffer (10 mM NaCl, 3mM MgCl2, 10mM Tris-Cl, pH 7.4). After cell lysis, the nuclei were digested with DNase I with 15 U for 10 min at 37oC. Digests were inactivated by the addition of 50 mM EDTA. RNA and proteins were digestion by RNase A (0.5 mg/ml) for 15 min at at 37°C and then by proteinase K (0.5 mg/ml) for 1 h at 65 oC. DNA was purified by phenol-chloroform extraction and ethanol precipitation. Libraries were prepared from 1-5 ng of DNA using the NEBNext ultra II DNA library prep kit.
GEO Accession
GSM4057519