GSM2478108: RNAseq stimulated CD8; Mus musculus; RNA-Seq
Cells were lysed by re-suspension in PXL buffer without SDS. Cell debris were pelleted and supernatants were biotinylated with EZ-Link-NHS-SS-Biotin. Poly-A RNA was enriched by pulldown with oligo-dT beads. RNPs were captured with M-280 Streptavidin dynabeads and RNAs were digested with RNAse T1 Linkers were ligated on while RNPs were immobilized on M-280 beads and released by proteinase K digestion, size selected by TBE-Urea PAGE gel, gel extracted, reverse transcribed and used for cDNA amplification
GEO Accession
GSM2478108