GSM1613687: small_rna_seq_miR-203_wt_epi; Mus musculus; miRNA-Seq
Total epidermal RNAs were isolated by treating skin with Dispase enzyme for 1 hr at 37 degrees C and physcially removing the epidermis from the dermis. The RNA was then extracted using Trizol (Invitrogen) according to the manufacturers instructions small-RNA-seq was performed by utilizing high efficiency 5' and 3' RNA adaptor ligation followed cDNA and PCR amplification as described in Zhang Z, Lee JE, Riemondy K, Anderson EM et al. High-efficiency RNA cloning enables accurate quantification of miRNA expression by deep sequencing. Genome Biol 2013;14(10):R109
GEO Accession
GSM1613687