GSM2397610: JEJ-13; Mus musculus; RNA-Seq
Intestinal epithelium scrapings were homogenized in TRIzol (Ambion, Waltham, MA) using a mixer mill 300 tissue homogenizer (Retsch, Germany). Total RNA was isolated according to manufacturers instructions with an additional phenol:chloroform extraction and resuspended in RNA storage solution (Ambion Inc., Austin, TX). RNA was quantified using a Nano-drop spectrophotometer (Thermo Scientific, Wilmington, DE) at 260 nm and purity was assessed by calculating the A260/A280 ratio. RNA quality was analyzed using the Caliper LabChip GX (Perkin Elmer, Waltham, MA). Libraries were were prepared using the Ovation Mouse RNA-Seq System 1-16 sample preparation kit (NuGen, San Carlos, CA), with an additional DNase step. Library sizes were confirmed using Caliper GX and quantified by the Kapa biosystems qPCR according to manufacturers instructions
GEO Accession
GSM2397610