GSM2478339: monoclonal Flag ChIP-Seq; 8567cells; 90C, minus tetracycline, plus corticosterone; Biological replicate 2; Mus musculus; ChIP-Seq
Cells were fixed in 1% formaldehyde, lysed and sonicated to small DNA fragments. IPs were performed with anti-BRG1 (1 μg, EPNCIR11A, made in collaboration with Epitomics, Burlingame, CA), or anti-Flag (4ug Sigma anti-Flag F1804) antibody. ChIP-Seq libraries were made by minimal PCR amplification cycles using the Illumina adaptor and PCR primers. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols.
GEO Accession
GSM2478339