GSM3004750: 96hW Larval Head Replicate 3 RNA-Seq; Apis mellifera; RNA-Seq
Larval head RNA was isolated using TRIzol (ThermoFisher, 15596018), followed by RNA Clean & Concentrator kit (Zymo Reasearch, R1015). Polyadenylated mRNA was isolated using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, E7490). Libraries were constructed with NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, E7420). Library quality was verified by running an Agilent High Sensitivity DNA chip (Agilent, 5067-4626) on a 2100 Bioanalyzer (Agilent Genomics).
GEO Accession
GSM3004750