GSM2901751: HeLa WT-2; Homo sapiens; RNA-Seq
Total RNA was extracted using RNeasy Mini Kit from QIAGEN. Integrity and quantity of RNA was evaluated by Agilent 2100, following the manufacturer's instructions. The mRNA and non-coding RNAs are enriched by removing rRNA using RNaseH. Mixed with the fragmentation buffer, target RNA were fragmented into short fragments and  cDNA was synthesized using the RNA fragments as templates by N6 random primer, followed by  end reparation and ligated with adapters. The quantity and quality of the cDNA libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies).
GEO Accession
GSM2901751