GSM1695173: H3K9me3_ChIPSeq_cont; Rattus norvegicus; ChIP-Seq
Lysates were extracted from one gram of crushed left ventricular tissue per group,  cross-linked with 0.5% formaldehyde, sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions (TruSeq ChIP Sample Preparation Guide Rev. A) accompanying TruSeq ChIP Sample Prep Kit. Briefly, DNA were blunted, phosphorylated, protruded with 3- 'A' base, and ligated with adapters. After adapter ligation DNA was isolated from an agarose gel and amplified with PCR. The DNA was captured for cluster generation. Libraries were sequenced on the HiSeq 2000 following the manufacturer's protocols.
GEO Accession
GSM1695173