Genome-wide changes in histone H3 lysine 27 tri-methylation associated to bud dormancy release in peach
An input sample containing sonicated DNA fragments from the dormancy-released bud sample, without antibody immunoprecipitation. ChIP fragments were amplified using the nano-ChIP-seq method. Briefly, input fragments were primed and amplified for 15 cycles, following the published protocol (Adli M, Bernstein BE, 2011, Whole-genome chromatin profiling from limited numbers of cells using nano-ChIP-seq, Nat Protoc 6:1656–1668). The ChIP DNA libraries were then constructed using ChIP-Seq DNA Sample Prep Kit (Illumina, San Diego, CA, USA) and subsequently sequenced as single-end 100 bp reads on an Illumina HiSeq 2000 at Genoscreen (Lille, France).