GSM2823681: Rhesus macaque donor AG08312 mock 3; Macaca mulatta; RNA-Seq
Total RNA extracted using Qiagen Rneasy Mini Kit per manufacturer's directions. The integrity of total RNA from the primate samples were assessed on Bioanalyzer 2100 using RNA 6000 Nano chip (Agilent Technologies, CA), only samples with RNA Integrity Number (RIN) greater than 9.0 were used for RNA-seq.  One microgram of total RNA from each sample was subject to poly-A containing RNA enrichment by oligo-dT bead and converted to cDNA then sequencing library using PrepX RNA-seq library kit on the automated Apollo 324TM NGS Library Prep System (Wafergen Biosystems, CA) according to the manufacturer's protocol.  Different DNA barcodes were added to each library.  The libraries were examined on Agilent Bioanalyzer DNA High Sensitivity chips for size distribution, quantified by Qubit fluorometer (Invitrogen, CA).  The RNA-seq libraries were pooled at equal molar amount and sequenced on Illumina HiSeq 2500 Rapid flowcells as single-end 75nt reads following the standard protocol.  Raw sequencing reads were filtered by Illumina HiSeq Control Software and only the Pass-Filter (PF) reads were used for further analysis.
GEO Accession
GSM2823681