Maintainer line for Rajalaxmi at Grain filling stage
Leaf samples from maintainer line (B) for hybrid, Rajalaxmi, CRMS32B at grain filling stage were harvested and sequenced. The libraries were prepared by fragmenting the mRNA followed by first strand cDNA synthesis, second strand cDNA synthesis, adaptor ligation, 200 bp cDNA fragments isolation and amplification (18 cycles of PCR). Libraries were sequenced (2 x 100 bp) on Illumina HiSeq 2000.