RNA-Seq analysis of ALL sample TARGET-10-PARMUC-03A-01R
Trizol was used to extract RNA from acute lymphoblastic leukemia samples of bone marrow or peripheral blood.  Total RNA had ribosomal RNA removed using Ribo-Zero rRNA removal beads and was converted into a cDNA library using Illumina TruSeq stranded Total RNA Kit with Ribo-Zero Gold.  After adapter ligation each cDNA library was purified and enriched by PCR amplification.  Each library underwent 100-cycle paired-end sequencing on the Illumina HiSeq 2000.  Four samples were multiplexed into each flow cell lane.