GSM1695251: CVB2 VP1Q164K 48h; Homo sapiens; Coxsackievirus B2 (strain Ohio-1); RNA-Seq
Briefly, the cells were washed with PBS and detached from the culturing flask by trypsinization. The trypsin was neutralized with serum and the cells spun down at 300 x g for 5 min, after which the supernatant was removed. The cells were lysed with the RLT buffer and homogenized through the use of QIAshredder spin column (Qiagen). 70 % ethanol was added and the sample was loaded on the RNeasy spin column and successively washed with RW1 and RPE buffer. The RNA was eluted with 50 µl nuclease-free water. The sequences were clustered using cBot and sequenced on HiSeq2500 (HiSeq Control Software 2.0.12.0/RTA 1.17.21.3) with a 2x101 setup in HighOutput mode. Bcl to Fastq conversion was performed using bcl2Fastq v1.8.3 from the CASAVA software suite. The quality scale is Sanger / phred33 / Illumina 1.8+
GEO Accession
GSM1695251