GSM1881526: cortex CLEAR-CLIP, biological rep 10a; Mus musculus; RIP-Seq
Cortex was dissected into ice cold PBS, triturated by passing twice though an 18G needle, and UV-irradiated.  AGO-cross-linked RNA was isolated with the CLEAR-CLIP protocol described in detail in the accompanying publication. AGO-cross-linked RNA was cloned by addition of pre-adenylated 3' linker and RNA 5' linker, then RT-PCR, as described in the published CLEAR-CLIP protocol. Products were amplified in a second round of PCR that added a 4 nt index (for multiplexing, see above) and 16 nt common primer sequence.  Raw files are demultiplexed on the basis of this index, but have not been further modified.
GEO Accession
GSM1881526