GSM2477206: shMXI1_Nx; Homo sapiens; RNA-Seq
Total RNA was extracted and purified using the RNeasy Mini Kit (Qiagen; 74106) Integrity and quantity of each RNA samples was determined by Advanced Analytical Technologies Fragment AnalyizerTM.  PolyA+ fractions were purified and randomly fragmented. After first strand cDNA synthesis using random primers, the second strand synthesis is performed.  The ends of the double stranded cDNA fragments are repaired and dATP ligated to the blunt ended fragments. Then, the sequencing adapters are ligated to the DNA fragments. Determination of size distribution and quantification of the sequencing was accomplished on the Advanced Analytical Technologies Fragment AnalyzerTM. The libraries were also measured on the Invitrogen QubitR Fluorometer. The results of these measurements were integrated to accurately determine the appropriate sequencing dilution.
GEO Accession
GSM2477206