Selective whole genome amplification of P. gaboni: Whole blood sample from Pan troglodytes
Erythrocytes were purified from venipuncture derived peripheral blood by Lymphoprep density gradient separation and mixed 1:1 (vol/vol) with RNAlater, transported at ambient temperatures and stored at -80C. Total extracted DNA was digested (MspJI, FspEI) and subjected to selective genome amplification using a Laverania specific SWGA primer set.  50 ng of the resulting product was amplified by selective amplification using a second Laverania specific SWGA primer set.  The 2nd round product was bead purified and prepared for illumina sequencing using the Nextera Mate Pair Library Preparation Kit with gel extraction to select for a 3 kb insert size.