full length non-chimeric of K0326Y : Various tissue
PacBio raw data from RSII platform and subreads from Sequel platform were processed following SMRT analysis ISO-Seq pipeline to gain Circular Consensus Sequences (CCS) with default parameters. After that, to classify full length non-chimeric (FLNC) reads and non FLNC reads using pbtranscript, and cluster them employing Iterative Clustering and Error correction (ICE) algorithm, then polished consensus isoforms using Arrow and divided into high-quality (HQ) and low-quality (LQ) isoforms with >=1 full length reads supporting and >=99% predicted accuracy. HQ isoforms were mapped by GMAP47, then redundant isoforms were collapsed by Cupcake ToFU. 247,616 collapsed HQ isoforms were obtained from this step.