AireKO medullary epithelial cells RNAseq replicate 2
Bulk RNAseq libraries were prepared using TruSeq (Illumina) following the manufacturer’s protocol from 5-10 × 104 sorted MECs (one mouse) per sample. Sequencing (single-end, 50bp) was performed on a HiSeq2000 (Illumina), and reads were aligned to mm10 using Tophat2.