GSM2898780: Mouse wild type erythroid cells replicate 1; Mus musculus; RNA-Seq
Samples for nascent RNA were collected after 12 h of differentiation. Cells were lysed in TRI Reagent (Sigma), and RNA was extracted according to the manufacturer's instructions. Samples were treated with DNase I using Turbo DNA-free DNase (Ambion). To obtain nascent RNA, fetal liver cultures were pulsed with 50 uM 4-thiouridine (Sigma) for 1 h followed by immediate lysis in TRI Reagent. Nascent RNA was then extracted according to the method described by Dölken et al. (Dölken, L. et al. High-resolution gene expression profiling for simultaneous kinetic parameter analysis of RNA synthesis and decay. RNA 14, 1959-1972 (2008)). NEBNext Ultra Directional RNA Library Prep Kit for Illumina.
GEO Accession
GSM2898780