Illumina HiSeq 2000 sequencing; A genetics screen highlights emerging roles for CPL3, RST1 and URT1 in RNA metabolism and silencing (mRNA profiling part)
A genetics screen highlights emerging roles for CPL3, RST1 and URT1 in RNA metabolism and silencing (mRNA profiling part)
17-day-old (DAS) shoot tissues of plants were harvested. Shoot tissues, consist of stems including the leaves, were harvested and immediately stored in liquid nitrogen. Seedlings were grown on half strength (1/2) MS medium containing 1% sucrose at 21˚C under a 16 h/8 h day/night condition. The RNA samples for mRNA sequencing analyses were extracted from 17-day-old shoot tissues of plants by the miRNeasy Mini Kit (Qiagen). RNAs were first selected via enrichment of their poly(A) tail to build the libraries with the TruSeq RNA Sample Preparation Kit (Illumina).
Experimental Factor: genotype
EMS mutation of RST1 in ERF6-GR background