GSM3145769: s2596 (Vehicle); Mus musculus; RNA-Seq
Agencourt RNAadvance kit was used for isolation of nucleic acids from frozen tissue and implemented on a Biomek FXP Laboratory Automation Workstation. Briefly the tissue is lysed, after which the RNA is immobilized onto the magnetic particles. The RNA is then treated with DNase and the contaminants rinsed away using a simple wash procedure. RNA isolates were eluted in a 55ul volume of RNase/DNase-free H2O. All RNA was stored at -80C. RNA quality was assessed by Agilent Bioanalyzer using the RNA 6000 pico kit TruSeq RNA Access Library Prep Kit was utilized for RNA-seq library prep. 50ng RNA was utilized as input. The method was automated on the Biomek FXP Laboratory Automation Workstation to facilitate enrichment of the coding regions of the transcriptome that are captured using sequence-specific probes to create the final library.
GEO Accession
GSM3145769