GSM4058382: WT_HC25_LNv_ZT14; Drosophila melanogaster; RNA-Seq
Fly brains were dissected at certain time points and processed as previously described (Kula-Eversole et al., 2010; Nagoshi et al., 2010). RNA from FACS sorted LNvs were extracted with PicoPure Kits. We synthesized 1st and 2nd strand cDNA from RNA first with Superscript III and DNA polymerase. Then we amplified the RNA by synthesizing more RNA from the cDNA template with T7 RNA polymerase. After the second round of cDNA synthesis from amplified RNA, the cDNA was submitted to HGAC at the University of Chicago for library preparation and sequencing. Library construction and sequencing followed the protocol at HGAC at University of Chicago.
GEO Accession
GSM4058382