GSM4120780: LN KO_4; Mus musculus; ATAC-seq
14 days upon tumour inoculation tumour-draining lymph nodes were isolated and Tregs were sorted (CD4+CD25+GITR+). Cells were lysed. Transposition reaction was performed with in-house Tn5 Transposase and DNA purification was effectuated by Qiagen MinElute kit and amplified using the NEBNext High-Fidelity PCR Master Mix Transposition reaction was performed with in-house Tn5 Transposase and DNA purification was effectuated by Qiagen MinElute kit and amplified using the NEBNext High-Fidelity PCR Master Mix
GEO Accession
GSM4120780