GSM1930795: Amel_Queen_brain1; Apis mellifera carnica; RNA-Seq
Total RNA was isolated from single brains using Trizol method. 50 µl Trizol were added to a single brain and the tissue was pestled. After incubation for 5 min at room temperature 10 µl chloroform were added. The solution was mixed for 30 sec and centrifuged for 15 min on 13,000 rpm. The upper phase was transferred into a new tube and 20 µl isopropanol were added and mixed. The solution was incubated for 10 min at room temperature and then centrifuged for 10 min at 13,000 rpm and 4°C. The pellet was dired for 5 min at room temperature and then diluted in 10 µl RNAse free water.
GEO Accession
GSM1930795