16S rRNA gene V4 region of bacteria: adult male high-fat-diet-fed Mus musculus (C57BL/6J ) fecal microbiota
Targeted PCR (95 °C, 2 min; 95 °C, 20 s, 55 °C, 15 s, 72 °C, 5 min for 30 cycles; 72 °C, 10 min). PCR products were cleaned using Agencourt AMPure XP system (Beckman Coulter).