GSM3003959: RRBS_control_3_p8; Homo sapiens; Bisulfite-Seq
DNA from human fibroblast was extracted using QIAamp DNA mini kit according to the manufacturer's instructions 500ng/sample of extracted DNA was digested with Msp1 enzyme (NEB #R0106M) Methylated adaptors (NEB #E7535L) were ligated with T4 ligase (NEB #M0202M) following by qPCR amplification with Illumina indexing primers. Libraries clean up and size selection was done using Agencourt® AMPure® XP magnetic beads (Beckman Coulter # A63881).
GEO Accession
GSM3003959