GSM3682900: miCLIP_ZCCHC4_KO_2; Homo sapiens; RIP-Seq
Total RNA from wild-type, mettl5-knockout, and zcchc4-knockout HCT116 cells was extracted in Tri Reagent (AM9738, Thermo-Fisher Scientific). Any contaminating genomic DNA was degraded with DNase I, and Poly(A) RNA was purified with an mRNA isolation kit (NEB #S1550) according to the manufacturer's recommendations. Five micrograms of poly(A)-selected RNA was then used as input for single-nucleotide resolution m6A mapping according to the miCLIP protocol.
GEO Accession
GSM3682900