GSM3145260: delta0595 rep1; Salmonella enterica subsp. enterica serovar Choleraesuis str. ATCC 10708; RNA-Seq
Total RNA extraction using an RNeasy Micro Kit (QIAGEN, GmBH, Germany). RNA integrity was assessed using a 2100 Bio-analyzer (Agilent, Foster City, CA). Total RNA was further purified by the RNase-Free DNase Set (QIAGEN, GmBH, Germany) after quantification. Total RNA samples were stored at -80°C until further use. The mRNA was used to prepare individually bar-coded (indexed) RNA-Seq libraries with a TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA, USA). Library QC and quantitation were performed on all individual libraries using the Qubit assay (Thermo Fisher Scientific, Waltham, MA) and the Agilent 2100 Bioanalyzer.
GEO Accession
GSM3145260