GSM4670169: Sox8fl/flSox9fl/fl-GP-14days [Growth plate cartilage]; Mus musculus; RNA-Seq
Knee joints were harvested from control and mutant mice and were snap frozen in OCT compound. Frozen sections were cut and then processed for laser-caputure microdissection. Articular and growth plate cartilage area were removed using laser-capture mirodissection, flash frozen on dry ice, and RNA was harvested using Qiagen Kit RNeasy Plus Micro Kit. SMARTer Stranded Total RNA-seq v2  Pico Input Mammalian kit (Takara Bio) was used with 10 ng of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM4670169