GSM2902399: FH6d_2; Mus musculus; RNA-Seq
Total RNA was isolated from C2C12 cells at the following time points: proliferating (70-80% confluence, D0), 3 days (D3), and 6 days (D6) after induction of differentiation. Total RNA was isolated using TRIzol according to the manufacturer's instructions. To enrich circRNAs, the total RNAs was treated with RNAClean XP Kit (Cat# A63987, Beckman Coulter, Inc., Kraemer Boulevard, Brea, CA, USA) and RNase-Free DNase Set (Cat#79254, QIAGEN, GmBH, Germany) following the manufacturer's instructions. 1000 ng RNA was used as the input for the VAHTSTM mRNA-seq v2 Library Prep Kit for Illumina ® (Vazyme, Inc., Piscataway, NJ, USA), and sequencing libraries were created according to the manufacturer's protocol.
GEO Accession
GSM2902399