Illumina NovaSeq 6000 paired end sequencing; RNA-seq of frontal cortex in a novel mouse model of Alzheimer's disease.
RNA-seq of frontal cortex in a novel mouse model of Alzheimer's disease.
Frontal cortex homogenates were extracted from experimental animals. Half of the mice were treated with 200ppm doxycycline in the chow for 4 months to reduce MAPT transgene expression and the others treated with control chow.  Total RNA was extracted from the frontal cortex using the Lipid Tissue Mini Kit (Qiagen). RNA quantity and quality was assessed using a Bioanalyzer 2100 (Agilent Technologies). All samples had RIN values > 7.  Barcoded RNA-seq libraries were prepared by Edinburgh Genomics using the Illumina TruSeq stranded mRNA-seq kit, according to the manufacturer's protocol (Illumina).
Experimental Factor: genotype
Mapt null
Experimental Factor: compound
doxycycline