RNA-Seq of Streptococcus mutans
Total RNA was extracted and purified. The RNAs were ligated to a 3' RNA adapter, and then a 5' adapter. The adapter-ligated RNAswere then subjected to reverse-transcription PCR and amplified. All RNA fragments (18-50 nucleotides) were isolated by PAGE. The library products were sequencing on an Illumina HiSeq 2500 platform.