Development and immunity of Micrioplitis bicoloratus in host Spodoptera litura
Total RNA was isolated from hemocytes using an RNeasy Plus Universal Mini Kit (QIAGEN, Maryland, USA), which is specific for genome DNA elimination, according to the manufacturer’s instructions. The concentration of each RNA sample was determined by measuring OD at A260/A280 using the NanoDrop 2000 and running agarose gel. High quality samples (with an A260/A280 ratio >2.0, A260/A230>2.0, concentration>500 ng/ul) showing 28S and 18S RNA bands clearly were stored at -80°C until use. RNA was prepared from at least two biological replicates and used for independent library preparations.