GSM2824238: WT_gp33+ CD8+ T cells_5763; Mus musculus; Bisulfite-Seq
gp33+CD44+ CD8+TCRb+ cells were sorted using flow cytometric sorting. Genomic DNA was generated using Qiagen AllPrep DNA/RNA kit as per manufacturer's protocol. Genomic DNA was bisulfite converted using the EZ DNA Methylation kit (Zymo Research). Base-pair resolution DNA Methylation was performed on control and TET2-deficient gp33+CD8+ T cells (n=4/genotype) as described in Akalin et al., PLoS Genet, 2012. Library was constructed as per protocol at Cornell Epigenomics Core
GEO Accession
GSM2824238