ML153
Genomic DNA was isolated from tissue samples using CpG MethylQuestTM DNA Isolation Kit, followed by RNase treatment to remove the contamination of RNA. The genomic DNA was then quantitated using Qubit dsDNA BR Assay Kit. The quality of DNA was examined using Agilent 2100 Bioanalyzer system and the qualified DNA was subjected to DNA library construction according to manufacturer’s protocol. The genome sequencing was performed by Centre for Genomic Sciences, The University of Hong Kong. In total, there were three paired-end libraries of insert size 250 bp, 500 bp and 750 bp, respectively, with sequencing depth estimated to be 56.8 X based on the genome size of Oryzias latipes.