NGS: snow microbial community
Melted snow samples were filtered through mixed esters of cellulose membrane filters and total genomic DNA was extracted. The V3V4 regions of the 16S rRNA gene were amplified using the primers 331F and 797R. The primers were modified with a forward overhang and a reverse overhang, which were necessary for dual index library preparation. The library was run on the Illumina MiSeq using the 2  300 bp paired-end approach.