GSM1612785: BS-seq HEK293 1; Homo sapiens; Bisulfite-Seq
For bisufite sequencing, total RNA was extracted using Trizol, and the polyA fraction isolated using oligodT dynabeads. Bisulfite conversion and desulfonation of RNAs was performed using the EZ RNA Methylation Kit (Zymo Research). Libraries were prepared using Illumina TruSeq RNA prep kits
GEO Accession
GSM1612785