GSM1881697: Gifu; Lotus japonicus; Bisulfite-Seq
Total DNA was extracted using the Macherey-Nagel Nucleomag96 Plant kit according to the manafactures instructions Libraries were prepared according to Lister et al., 2008 (Cell) with the following modifications: Bisulfite conversion was preformed using Qiagen epitech kit. PCR amplification was done using KAPA HIFI Uracil plus polymerase together with bar-coded primers (Illumina Tru-seq barcodes 1-48) to allow muiltiplexing
GEO Accession
GSM1881697