GSM4173555: FarS rep 2 [FarS target identification]; Vibrio cholerae; RNA-Seq
RNA was isolated using Tri-reagent according to standard protocols. Prepared RNA was DNase digested and cleaned up using standard nucleic acid precipitation procedures. Depletion of ribosomal RNA was performed using the Ribo-Zero rRNA Removal kit (Epicentre) for gram-negative bacteria. Integrity of the prepared RNA was tested using a Bioanalyzer. cDNA libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB E7760), according to the manufacturer's instructions. Quality of the prepared libraries was tested using a Bioanalyzer.
GEO Accession
GSM4173555