28S sequences of Skeletonema community
A 325-bp 28S gene fragment were PCR amplified from BioSamples' genomic DNA using a pair of 28S primer modified by adding illumina adaptors to the 5' ends. PCR amplicons were processed for sequencing DNA library using Illumina Nextera kit and sequenced in a multiplexed sample on a single flow cell of Miseq platform for 250 bp paried-end reads.