GSM3737057: AMB only rep 3; Cryptococcus neoformans var. grubii H99; RNA-Seq
For transcriptomic analysis, RNA was extracted from the following treatment conditions: i) AMB only, ii) growth control of (i), iii) AMB+LF and iv) growth control for (iii). Growth controls were extracted at the same time as its corresponding treatment and LF only treatment was excluded from transcriptomic analysis. RNA extraction was performed from freeze dried cells via bead beating and the Qiagen RNeasy mini kit as detailed in Pang et. al. (Scientific Reports, 2017. 7: 40232). RNA was sequenced at the Ramaciotti Centre for Genomics using the Illumina HiSeq 2000 to generate 100 bp paired-end reads. For the AMB only treatment, the AMB+LF treatment and their corresponding matching controls, the TruSeq RNA Library prep kit v2 (Illumina) with a poly-A pulldown was used according to the manufacturer's instructions, with 2 μg of total RNA for each of the three biological triplicate samples as input. The libraries were enriched using 15 cycles of PCR and the insert size ranged from 80 to 330 base pair (bp). The libraries were randomized and sequenced in two separate lanes using HiSeq 2000 with the TruSeq v3 SBS reagents to generate 100 bp paired-end reads. The data was demultiplexed using Casava 1.8.2.
GEO Accession
GSM3737057