GSM2478367: p53 wt rep1; Mus musculus; RNA-Seq
Pancreas was resected, dissociated and labeled. Living CD133+ ductal epithelial cells that make up the PanINs were sorted and RNA was harvested using PicoPure RNA Isolation Kit. Illumina TruSeq Stranded RNA LT Kit (Cat#15032612) was used and 1 µg of total RNA was entered for the construction of sequencing libraries. To achieve a minimum of 1 µg of total RNA for each library, RNA samples from 2 mice of the same genotype were combined The libraries were prepared using the manufacturer's standard protocol
GEO Accession
GSM2478367