GSM2898134: Alum+KLH; Mus musculus; RNA-Seq
Dorsal root ganglion (DRG)  were harvested from the thoracic and lumbar regions of mice immediately following euthanasia, as described previously (Malin et al 2007, Nat. Protoc. 2: 152–160). To enrich for DRG neurons, DRGs were digested with 1µg/mL collagenase/dispase (Roche) in neurobasal medium (Gibco, Thermo Fisher Scientific, Waltham MA) for 1 hour at 37°C on a rotator-shaker.  Following digestion, DRGs were washed with HBSS (Gibco) and filtered through a 70µM strainer.  The cell suspension was layered on a 15% BSA in HBSS, and centrifuged at 800rpm for 20 minutes without brake.  The cell pellet was re-suspended in neurobasal medium. B lymphocytes were depleted from cell preparations using DynabeadsTM mouse pan B purification kit (Invitrogen, USA). RNA libraries were prepared for sequencing using the Truseq Stranded mRNA prep kit following standard Illumina protocols
GEO Accession
GSM2898134