GSM2476821: Y537S-E2-rep1-S2-5-MP2867; Homo sapiens; ChIP-Seq
Cell lines before ChIP were then either introduced to White Medium DMEM with or without supplemental E2 or Doxycycline, Cells were sonicated, then cell lysate was incubated with antibody to extract protein-DNA complex of interest, Library Preparation is performed using the Rubicon 48S kit. All of the ChIP-seq performed was done using a combination of Estrogen Receptor ab-10/Thermo Scientific/# MS315PABX and Estrogen Receptor HC-20/Santa Cruz Biotechnolofy/sc-543.
GEO Accession
GSM2476821