Hyalella azteca Experimental Run
The samples were analyzed using genomics:PicoGreen, purified using AmPure beads, bound to library immobilization beads, and the unbound strand was melted off to produce sstDNA, all of which is described in the GSFLX Library Construction manual. The sstDNA was analyzed with an Agilent mRNA Pico chip. Each library was titrated seperately using titration emPCR, after which an LR70 2 Region datarun was done for each library.