Pseudocohnilembus persalinus genome sequencing mate-pair library
Illumina adapters were ligated onto the genomic DNA, then DNA fragments (2kb) were selected using agarose gel electrophoresis. Libraries for Illumina were PCR amplified using Phusion polymerase, denatured with sodium hydroxide and diluted in hybridization buffer. The prepared libraries were loaded onto the flowcell and perform sequencing.