GSM2478308: KO3; Mus musculus; RNA-Seq
After Hippocampus dissection, HA-Rpl22 were immunoprecipitated. Total RNA was extracted from ribosome-mRNA complexes using RNeasy Microkit (Qiagen) followed by in-column DNase treatment to remove genomic DNA contamination. Quality and quantity of RNA samples were both assessed using Agilent Bioanalyzer 2100 (Agilent Technologies). Six biological replicates, each one composed of a pool of 4 to 6 mice, were used for RNAseq analysis. RNA-Seq libraries were prepared with the 'TruSeq Stranded RNA sample preparation Kit' (Illumina) according to the manufacturer's instructions. Stranded RNA-seq
GEO Accession
GSM2478308