GSM3003470: h1D-h2D_Sen1-AID+Aux_Rad52ChIP-seq_2; Saccharomyces cerevisiae; ChIP-Seq
Cells were fixed with paraformaldehyde (final 1%) for 1h. Glycine was added to stop the reaction at 0.125M for 10'. After cell lysis, chromatin was sheared 20 times for 45 s each using a Covaris S2. The library was prepared using the Accel-NGS 1S Plus DNA Library Kit (Swift Bioscience) following the manufacturer protocol.
GEO Accession
GSM3003470