With the purpose of gaining some insights on coffee resistance process, a RNA Illumina sequencing approach was used to characterize the transcriptional profile of two coffee genotypes, respectively resistant (Catimor 88) and susceptible (Caturra) to C. kahawae, during the early stages of the infection process.
Total RNA was isolated from hypocotyls of all samples with SpectrumTM Plant Total RNA Kit (Sigma-Aldrich, USA), according to the manufacturer’s instructions. Total RNA purity and concentration was measured at 260/280 nm and 260/230 nm using a spectrophotometer (NanoDrop- 1000, Thermo Scientific), while RNA integrity was verified by gel electrophoresis.