GSM4059084: WT CM [77329]; Homo sapiens; RNA-Seq
Cells from different genotypes/differentiations were harvested in Trizol (Life Technologies) and total RNA was isolated and DNase-treated using the Direct-zol RNA miniprep kit as per manufacturer's instructions (Zymo Research). In the case of cardiomyocytes, cells at D10-D11 of differentiation were used, while in the case of NCCs, cells were obtained by collecting migratory cells from dissected and re-plated rosettes. polyA-enriched RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM4059084