GSM4333174: PID115star_GMP; Homo sapiens; RNA-Seq
Human CD34+ hematopoietic stem/progenitor cells were enriched from mononuclear cells using immunomagnetic beads according to manufacturer instructions (CD34 MicroBead Kit; Miltenyi Biotec). Fluorescence-activated cell sorting and antibodies specific for CD2, CD3, CD4, CD7, CD8, CD14, CD19, CD56, CD10, CD11b, CD20, CD235a, CD34, CD38, CD123, CD45RA were used to isolate the various cell types (HSC, CMP/MEP, CMP, GMP, MEP). 10'000 cells of each subpopulation were sorted into RNeasy lysis buffer containing beta-mercaptoethanol. Total RNA was purified according to manufacturer instructions using the RNeasy Plus Micro Kit (Qiagen). RNA libraries were prepared for sequencing using SmartSeq2-Picelli protocol.
GEO
GSM4333174
GEO Accession
GSM4333174