GSM1398745: PAT081; Homo sapiens; MBD-Seq
Peripheral blood mononuclear cell (PBMC) genomic DNA was extracted using the Qiagen DNeasy blood and tissue kit according to the manufacturer's protocol. After sonication of DNA with Diagenode bioruptor, enrichment of methyl-CpG containing DNA was performed with the Methylminer DNA Enrichment Kit (Life Technologies) according to the manufacturer's protocol with an elution buffer salt concentration of 600 mM. Library preparation was undertaken using 5 ng of methyl-enriched DNA using the NEBNext DNA Library Preparation Kit for Illumina (New England Biolabs). Library DNA was quantified with MultiNA capillary electrophoresis instrument with DNA-500 kit. Cluster generation was done using library DNA at a concentration of 10 pM on Cluster Station (Illumina). Sequencing by synthesis was performed on Genome Analyzer IIx (Illumina) for 36 cycles using RTAv1.8 base calling software.
GEO
GSM1398745
GEO Accession
GSM1398745