targeted DNAseq human lung tumor adult: Sample 3199-PM-29
Standard genomic DNA was sonicated using a protocol designed for a 200 bp target insert size on the Covaris LE220.  Each library was prepared using 1 μg of gDNA following the manufacturer's protocol.  Each library was amplified by ligation-mediated PCR (eight cycles) and hybridized to custom probes.  The final amplification was completed of the post-captured library by ligation-mediated PCR (14 cycles), according to the manufacturer's protocol following the SeqCap EZ library preparation guide (NimbleGen, Madison, WI).  The libraries were sequenced using the HiSeq 4000 with 150 bp paired end reads targeting 40M reads per sample.  RTA (version 2.4.11; Illumina) was used for base calling and analysis was completed using MultiQC v1.7
NCI-phs001811
3199-PM-29