GSM4174099: METTL3_Control_MeRIP_r1 [HEC-1-A: MeRIP]; Homo sapiens; OTHER
Total RNA was isolated from chromatin-associated fraction of HEC-1-A cells. Non-ribosome RNA was further enriched from total RNA by using RiboMinus transcriptome isolation kit (Invitrogen). 1 μL 1:1000 diluted m6A spike-in from EpiMark N6-Methyladenosine Enrichment Kit (NEB #E1610S) was added to 1 μg non-ribosome RNA, followed by fragmentation according to previously published protocols. m6A-IP was performed using EpiMark N6-Methyladenosine Enrichment Kit following the manufacturer's protocols. Library preparation was performed by using SMARTer Stranded Total RNA-Seq Kit v2 (Takara) according to the manufacturer's protocols.
GEO
GSM4174099
GEO Accession
GSM4174099