GSM4671805: SK-BR-3 parental cell; Homo sapiens; RNA-Seq
Randomly fragmented the purified RNA for sequencing with a short read and reverse-transcribed the cleaved RNA fragment into cDNA. 6 µg of total RNA were primed with 2 µl of DNA primer at 70°C for 10 min, then reversed transcribed at 42°C for 1 h in the presence of RTase (Invitrogen), and dNTP. Attached different adapters to both ends of the prepared cDNA fragment and ligated them. cDNA fragments were amplified with PCR. Seuencing service was provided by Macrogen Inc. Hybrid service was also provided by Macrogen.
GEO
GSM4671805
GEO Accession
GSM4671805