16S Amplicon:Balsam Fir Larva 4
"Briefly, DNA extracted from larvae midguts was amplified by PCR using 16S V6-V8 fusion primers, followed by amplicon purification. Then, for each of the three treatments, equimolar amounts of the 12 replicate amplicons were pooled and loaded onto 1/8th of a sequencing plate and sequenced on a Roche 454 GS-FLX + platform."
Universite Laval
Balsfir_MID4