RNA-Seq of crotalus viridis: adult male testis
Total RNA was extracted from snap-frozen tissue using Trizol Reagent followed by RNA precipitation from the aqueous phase using isopropanol. Individual Illumina mRNA-seq libraries were constructed using either a NEB NextRNAseq kit with poly-A selection, RNA fragmentation, cDNA synthesis, and indexed Illumina adapter ligation. RNAseq libraries were quantified using a BioAnalyzer (Agilent), pooled in equal molar ratios in various multiplex arrangements, and sequenced on an Illumina HiSeq 2500
SUB6988141
CVV_2016