GSM4173795: LS180_IgG_rep2; Homo sapiens; ChIP-Seq
Chromatin immunoprecipitation (ChIP) was performed using the Active Motif ChIP kit (Active Motif, Carlsbad, CA, USA) According the instruction by the manufacturer. Briefly, 5 × 107 LS180 cells grown in 15-cm plates were cross-linked with 1% formaldehyde, and cells were lysed and sonicated. Protein–DNA complexes were immunoprecipitated with control IgG (Santa cruz, Cat#2028) or anti-FOXA1 (Santa Cruz, Cat# sc-6553) antibody. The IP material was washed and heated at 65°C overnight to reverse crosslinks.
GEO
GSM4173795
GEO Accession
GSM4173795