GSM4221529: ATACseq, D0, Replicate 2; Mus musculus; ATAC-seq
For ATAC-Seq, 50 000 cells were lysed and treated with Tn Transposase, then purified using MinElute PCR purification kit (Qiagen). For ChIP-Seq, histone-DNA complexes were isolated from sonicated nuclei using bead-immobilized antibody. DNA was isolated by phenol/chloroform extraction. For RNAseq, cells were lysed in Trizol, and total RNA was isolated using Qiagen columns. RNA was treated with DNAse once on-column and once in-tube using Turbo DNAse kit (Thermo FIsher). ATAC-Seq libraries were prepared using Nextera PCR primers (Illumina). ChIP-Seq libraries were prepared using TruSeq ChIP Libary Preparation Kit (Illumina). RNAseq libraries were prepared using KAPA Stranded RNA-Seq with RiboErase Kit (Roche/KAPA Biosystems)
GEO
GSM4221529
GEO Accession
GSM4221529