16S Illumina MiSeq sequencing of sponge-associated microorganisms in Vietnam
Genome DNA was extracted using the ZymoBead™ Genomic DNA Kit (Zymo, USA), and then the V4 region of 16S rRNA genes were amplified by PCR protocol as previously described (Caporaso et al. 2012) using the bacterial primers 515F/860R as part of the Earth Microbiome Project (EMP) (Gilbert et al. 2014). Samples were mixed and pooled in equal concentrations to ensure equal representation of each sample, and were sequenced by Genome Sequencer Illumina HiSeq2500 at the GATC Biothech, Germany
SUB1971474
Dat_VN_sponges_AXT3