Metagenome of oxygen disturbed sulfate reducing bioreactor - Oxy-2
DNA was extracted from a 10 ml sample of all six cultures (Zhou et al., 1996). For metagenome shotgun sequencing, 1.5 μg of the extracted DNA per sample were mechanically fragmentend using Nebulizers (Roche) with 32 psi applied for 3 min, in 500μl nebulization buffer (Roche). The fragmented DNA was purified using MinElute PCR purification columns (Qiagen) and eluted in 50μl low TE (Life Technologies). The entire eluate was used for the preparation of barcoded PGM sequencing libraries with the Ion XpressTM Plus gDNA Fragment Library Preparation kit (manual Pub. No 4471989, Rev. M, May 2013; Life Technologies). Library insert size was between 350 and 400 bp. Libraries were sequenced with the Personal Genome Sequencer (PGM) on 318 Chips (pooled with other samples), using the chemistry for 400 bp libraries. Base calling was performed with the Torrent Suite v4.0.2 software, with default settings.
CeBiTec Bielefeld
Zainab_BioreactorOxy_DNA-2