infant gut metagenome by Illumina (Hiseq) sequencing
The shotgun genomic libraries were prepared with Illumina's NexteraXT™ DNA Sample Preparation Kit. Each library was quantitated by qPCR, pooled and the pool sequenced on 4 lanes for 151 cycles from each end of the fragments on a HiSeq2500  using a TruSeq SBS sequencing kit and analyzed with Casava1.8.2.
National University of Singapore
Hiseq_165