Indian muga silkworm
WGS library was prepared  with  Illumina-compatible NEXTflex Rapid DNA  sequencing  kit. DNA was sheared using Covaris S2 sonicator to generate distribution 150 bp to 250bp for short insert and 200 bp to 350 bp. Purified  fragments  were  end-repaired,  adenylated  and  ligated to Illumina multiplex barcode adaptorsas per NEXTFlex Rapid DNA sequencing kit. Adapter ligated DNA was purified and amplified for 6 cycles of PCR. Final PCR product was purified with Highprep beads and QC.
SUB5891520
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