RNA-Seq isolated from skin fibroblast cells treated with Novartis compound       NVS_SM1
Total RNA was isolated using the Qiagen RNeasy Mini isolation kit. RNA-Seq libraries were prepared using the Illumina TruSeq RNA Sample Prep kit v2 and sequenced using the Illumina HiSeq2500 platform. Each sample was sequenced on four different lanes belonging to the same flow cells to a length of 2x76 base-pairs (bp). The quality of the generated reads was assessed by running FastQC (version 0.10.1) on the FASTQ files. The average quality per base in Phred score was computed for each sample. Reads were of high quality (mean Phred score > 28 for all base positions). FASTQ files from same sample loaded on different lanes were concatenated into a single FASTQ file/sample.
Novartis Institute for Biomedical Research
NVS_SM1_3