Saccharomyces cerevisiae IMX774
DNA was isolated from yeast cells harvested from shake-flask cultures of strain IMX774 on synthetic medium (20 g L-1 glucose) using a Qiagen Blood & Cell Culture DNA kit (Qiagen, Germantown, MD), following manufacturer's specifications. Paired-end sequencing (22 mln reads) was performed  on a 350-bp PCR-free insert library using an Illumina HiSeq PE150 sequencer (Novogene Company Limited, Hong Kong) with a sample size of 3.3 Gb, accounting for a total coverage of 275x. Sequence data was mapped to the CEN.PK113-7D genome, to which the sequences of the pDAN1-prk-tPGK1, pTDH3-cbbm-tCYC1, pTEF1-groEL-tACT1, and pTPI1-groES-tPGI1 cassettes were manually added. Data processing and chromosome copy number analysis were carried out as described previously in:  Bracher JM, de Hulster E, Koster CC, van den Broek M, Daran J-MG, van Maris AJA, Pronk JT. Laboratory evolution of a biotin-requiring Saccharomyces cerevisiae strain for full biotin prototrophy and identification of causal mutations. Appl Environ Microbiol. 2017.  Verhoeven MD, Lee M, Kamoen L, van den Broek M, Janssen DB, Daran J-MG, van Maris AJA, Pronk JT. Mutations in PMR1 stimulate xylose isomerase activity and anaerobic growth on xylose of engineered Saccharomyces cerevisiae by influencing manganese homeostasis. Sci Rep. 2017;7:46155.
Delft University of Technology
IMX774