GSM1619141: CI1 spleen_16; Homo sapiens; RNA-Seq
After washing with 0.9% NaCl (Fresenius Kabi, France), the organs and tissues were snap-frozen in buffer RLT (Qiagen, Germany) and stored at -80°C until further use. The organs and tissues were homogenized using a pestle or a syringe needle followed by the QIAshredder homogenizer (Qiagen, Germany). Total RNA was isolated with the RNeasy Kit (Qiagen, Germany) including on-column DNase digestion. The DeepSAGE libraries were generated as previously described (Mastrokolias et al., 2012; GSE33701). Each library (8 pM) was loaded on a v3 flowcell and sequenced on an Illumina HiSeq2000 sequencer (Illumina, USA).
GEO
GSM1619141
GEO Accession
GSM1619141