Metatranscriptome of sulfate reducing bioreactor 3 - Nit-1 before nitrate disturbance
RNA was extracted from 2 ml of culture. The extracted RNA was treated with DNase (Promega) in the presence of RNasin (Promega) and purified using RNeasy MinElute spin columns (Qiagen). Prior to library preparation, rRNA was depleted from approx. 5 µg total purified RNA using the Ribo-ZeroTM Removal Kit (Bacteria) (epicentre). The rRNA-depleted sample was then used for library preparation with the Ion total RNA-Seq Kit v2 (Life Technologies) following the protocol for whole transcriptome library preparation. The generated cDNA library were sequenced with the Personal Genome Sequencer (PGM) on a 318 Chip, using the chemistry for 200 bp libraries. Base calling was performed with the Torrent Suite v3.6 software, with default settings.
CeBiTec Bielefeld
Zainab_BioreactorNitr_RNA-3