Amplicon-seq of mus musculus brain-passaged chikungunya live attenuated vaccine candidates
Virus was serially passaged in suckling mouse brain and total RNA was extracted and depleted of rRNA using NEB Human/Mouse/Rat rRNA depletion kit. 10^6 genomes as measured by qRT-PCR were used as input for PrimalSeq multiplexed amplicon-based Illumina library generation pipeline using custom primers designed with PrimalScheme.
SUB7609250
CHIKV-181/25-5-1.2