RNA-Seq of Temnothorax americanus: Larva, Colony_Y_695
RNA extraction of all samples was conducted in November 2016 using the RNAeasy mini extraction Kit (Qiagen, Inc.). For each species, we analyzed a medium-sized larva, an unpigmented worker pupa, a male, an adult worker, and a fertile queen from three different colonies each (N=30). We extracted RNA from whole bodies, as we had no prior assumptions as to which tissue differed most strongly in gene expression between our groups. To determine the sex of the larvae, we tested heterozygosity. For T. longispinosus the test shows clearly that all larvae are female. For T. americanus the samples show a heterogeneous pattern of heterozygosity, e.g., lowest heterozygosity in two of the queens, thus we cant clarify whether the larvae in this species are male or female. Library construction and sequencing was conducted at BGI Hong Kong on an Illumina HiSeq 4000. For rRNA depletion a poly-A selection was conducted. With a read length of 100 bp the libraries resulted in a total of 40 million paired-end reads (4 GB) for each sample.
SUB6933222
Temnothorax_16