GSM4221498: bovine in treat group 2; Bos taurus; miRNA-Seq
Total Exosome Isolation kit (from other body fluids) (Invitrogen, Catalog Number: 4484453) was used for the isolation of milk EVs. The miRNeasy Mini Kit (QIAGEN, Catalog Number: 217004) was used to extract EV-miRNAs. TruSeq Small RNA Sample Prep Kits (no. RS-200-0012, Illumina), Qubit dsDNA Assay Kit (no. Q328520, Life Technologies), Qubit RNA Assay Kit (no. Q32852, Life Technologies), SuperScript II Reverse Transcriptase (no.18064-014, Invitrogen) and DNA loading dye (no.LC6678, Invitrogen) were utilized in this step, following the manufacturer's instructions. Briefly, 3' adapter and 5' adapter were ligated to the end of the RNA molecules. Then the libraries were reverse transcribed and PCR amplified subsequently. The cDNA constructs were purified and recovered, and the libraries was checked and normalized eventually.
GEO
GSM4221498
GEO Accession
GSM4221498