Amplicon sequencing of NRC4 KO
DNeasy Plant DNA Extraction Kit (Qiagen) was used to extract the genomic DNA of NRCs knockout N. benthamiana lines. Primers aligning to each NRC gene were used to amplify the region targeted by the sgRNAs in multiplex PCR reactions. The amplified DNA fragments at 150bp were then sequenced.
SUB6429431
NRC4