Genome Sequencing of Pythium irregulare CBS 494.86
P. irregulare strain CBS 494.86 was acquired from the CBS-KNAW Fungal Biodiversity Centre, and it was and cultivated on potato dextrose agar (PDA) for 3 days and then inoculated in YPDO (g/L: yeast extract 1,25, peptone 25, glucose 3, oatmeal 2) medium for 5 days at 29 °C. The DNA of the biomass was extracted following the Graaff et al. (1988) protocol. Genomic DNA libraries for Next Generation Sequencing were produced using the Nextera DNA library preparation kit. Sequencing was carried out on a Illumina HiSeq 2500 instrument, using paired-end chemistry (2x100bp) at the NGS core facility of the Brazilian Bioethanol Science and Technology Laboratory (CTBE)
Laboratorio Nacional de Ciencia e Tecnologia do Bi
CTBEPIRR