Transcriptome of Nephrotoma ferruginea
Messenger RNA (mRNA) was isolated using the Dynabeads mRNA Purification Kit (Illumina TruSeq RNA Sample Preparation Kit v2, San Diego, CA, USA) and then sheared using divalent cations at 72°C. These cleaved RNA fragments were transcribed into first-strand cDNA using SuperScript™ II Reverse Transcriptase (Invitrogen, Carlsbad, CA, USA) and first strand mix (Illumina TruSeq RNA Sample Preparation Kit v2, San Diego, CA, USA). The second-strand cDNA was subsequently synthesized using RNase H (Invitrogen, Carlsbad, CA, USA) and DNA polymerase I (Invitrogen, Shanghai, China). The double-stranded cDNA then underwent end-repair, a single ‘A’ base addition, adapter ligation, purified by magnetic beads. At last, the product was indexed and PCR amplified to finalize the library preparation for the paired-end cDNA.
ZMB
ANIsrmTAAYRAAPEI-201