pLAS-3
To study cell type-specific expression in the leaf boundary region, we employed the Translating Ribosome Affinity Purification (TRAP) approach together with RNA-seq. From 7 DAG seedlings, we have obtained translatomes in the LAS-expression organ boundary cells and AS1-expressing leaf primordia and cotyledon cells. Total RNA, and subsequent poly(A)+ was isolated from each replicate, and was subject to RNA-seq library preparation as described before. Libraries were sequences as 50-mers using HiSeq2000 (Illumnia, San Diego, CA) with standard settings. Three independent biological replicates were included for each cell type.
Institute of Genetics and Developmental Biology, C
pLAS-3