Cascade Hops PacBio CLR RSII
Cascade clones grown in greenhouse under controlled conditions. ~100 ug young leaves harvested and placed immediately on ice. DNA extracted with modified Qiagen DNAeasy mini-prep kit. Chemical precipitation and glass hooks substituted for spin columns to prevent shearing. SMRTbell libraries constructed from unsheared DNA with 10kb size selection (BluePippin). Quality assessed with pulse field gels. Sequenced on RSII with P6C4 chemistry.
SUB6234494
CascadeHops_RSII_62