GSM4492235: D1 RNA-seq; Homo sapiens; RNA-Seq
Cells were collected, and RNA was isolated and purified with the Trizol (Invitrogen).then RNA was isolated and purified with the Trizol (Invitrogen). RNA purity was assessed using the ND-1000 Nanodrop. Each RNA sample had an A260:A280 ratio above 1.8 and A260:A230 ratio above 2.0. RNA integrity was evaluated using the Agilent 2200 TapeStation (Agilent Technologies, USA) and each sample had the RIN above 7.0. Briefly, rRNAs were removed from Total RNA using EpicentreRibo-Zero rRNA Removal Kit (illumina, USA) and fragmented to approximately 200bp. Subsequently, the purified RNAs were subjected to first strand and second strand cDNA synthesis following by adaptor ligation and enrichment with a low-cycle according to instructions of NEBNext® Ultra™ RNA Library Prep Kit for Illumina (NEB, USA).(Vazyme, Nanjing, China) to enrich mRNA, and RNA library was performed by using VAHTS mRNA-seq v2 Library Prep Kit from Illumina. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO
GSM4492235
GEO Accession
GSM4492235