GSM3927619: H25_GO; Ovis aries; RNA-Seq
RNA extraction was performed using Trizol® reagent, and quality of the obtained RNA was determined using a nanodrop spectrophotometer (OD260/OD280) to determine purity, as well as an agarose gel electrophoresis to observe potential RNA degradation and contamination. The RNA integrity was assessed using an Agilent Bioanalyzer, with RNA integrity number (RIN) values between 8 and 10. All the RNA-sequencing procedures were performed by Novogene Bioinformatics Technology Co., Ltd (Beijing, China). Sequencing libraries were prepared using a Poly-A tail capture method and sequenced with Illumina's HiSeq 3000.
GEO
GSM3927619
GEO Accession
GSM3927619