GSM2667199: Segment_02_rep1and2_PCR2; Saccharomyces cerevisiae; OTHER
To extract plasmid DNA, 5 billion cells from each sample were incubated in 300 μL of zymolyase buffer (1 M sorbitol, 0.1 M sodium acetate, 60mM EDTA (pH 7.0), 2 mg/ml zymolyase, 1% 2-Mercaptoethanol) at 37°C for 3 h. The plasmid DNA was purified from the obtained spheroplasts using QIAprep Spin Miniprep Kit (QIAGEN, 27104) according to the manufacturer's protocol. NGS libraries were prepared from 100 ng of the purified DNA amplicons using Ovation Rapid DR System (Nugen, 0319-32) according to manufacturer's instructions.
GEO
GSM2667199
GEO Accession
GSM2667199