SSR-Seq of Vitis vinifera
Two PCR amplification rounds were performed according to the protocols of Gohl et al. 2016 and Vartia et al. 2016. The modified protocol consisted of amplification by locus-specific primers (forward and reverse) that were adapted to contain universal primer sequence. Later two barcoded universal primers were included to incorporate two barcodes into each of the resulting amplicons.
SUB7773924
VitisBulk