Stenamma  megamanni 1 UCE
Randomly sheared DNA to a target size of approximately 650 bp (range 400-800 bp) by sonication (Qsonica Inc. Q800 or Diagenode BioRuptor), and we input 50-500 ng sheared DNA (418 ng mean) into a modified genomic DNA library preparation protocol (Kapa Biosystems) that incorporated "with-bead" cleanup steps (Fisher et al. 2011) using a generic SPRI substitute ((Rohland & Reich 2012); hereafter SPRI). This protocol is similar to the Kapa Biosystems protocol that uses commercial SPRI chemistry for cleanup and includes end-repair, adenylation, and T/A ligation steps, except that the Fisher modification does not remove and replace SPRI beads between each step. We also substituted custom-designed sequence-tagged adapters to the ligation reaction (Faircloth & Glenn 2012).
University of California
SAMN02803933
index
BFIDT-162:GCAATTACCG