GSM1939694: ca1d_1 (Hipposeq); Mus musculus; RNA-Seq
Manual sorting to purify for fluorescent neurons from microdissected slices was performed according to previous methods (Hempel et al., 2007). Total RNA was isolated from each sample using PicoPure RNA Isolation kit (Life Technologies, Frederick, MD) including the on-column RNAseq-free DNAseI treatment (Qiagen, Hilden, Germany) following the manufacturers' recommendations.  Eluted RNA (11 ul) was dried in a speed vac to approximately 2-4 ul.  ERCC control RNAs (Life Technologies) were added using 1 ul of 1:100,000 dilution for every 50 cells.  cDNA was amplified from this input material using Ovation RNA-seq v2 kit (NuGEN, San Carlos, CA).  Approximately half of the resulting cDNA was used to make the sequencing libraries using the Ovation Rapid DR Multiplexing kit (NuGEN).
GEO
GSM1939694
GEO Accession
GSM1939694