GSM4333065: Pool10; Homo sapiens; RNA-Seq
Saliva was kept at -20°C before extraction. Saliva was centrifuged at 4°C for 15 min at 2600 g. RNA was extracted from the supernatant using the NucleoSpin RNA XS Kit (Macherey-Nagel) according to the manufacturer's instructions, with elution in 20 µl. 10 RNA samples were pooled and ethanol precipitation was applied. Library preparation was done using KAPA stranded mRNA-seq library kit (Roche KK-KK8421) with a modification to the protocol - the RNA library preparation was initiated from the fragmentation step, after which the regular protocol was followed. Library quality control was done using Qubit 4 (Invitrogen) and TapeStation 2200 (Agilent). Library was diluted to 4 nM and sequenced on NextSeq 500, using the NextSeq high output kit v2, 75 cycles (Illumina).
GEO
GSM4333065
GEO Accession
GSM4333065