The malignant transformation roadmap of human neural stem cells toward high grade glioma
Genomic DNA was extracted from brain tissues by Blood & Tissue genomic DNA extraction Kit (Tiangen). Sequencing libraries were generated using Agilent SureSelect Human All Exon kit V6 (Agilent Technologies, CA, USA) following manufacturers recommendations. Briefly, fragmentation was carried out by hydrodynamic shearing system (Covaris, Massachusetts, USA) to generate 180-280 bp fragments. The sequencing libraries were constructed on a cBot Cluster Generation System using Hiseq PE Cluster Kit (Illumina) according to the manufacturers instructions. After cluster generation, the DNA libraries were sequenced on Illumina Hiseq platform and 150 bp paired-end reads were generated (Novogene Co, Ltd, Beijing, China).
SUB7020539
TN-End-2-SVZ