Ion Torrent S5 sequencing
The body surface bacterial community of a stressed (non-lesioned, devoid of spines) individual was sampled by scraping the body surface with a sterile scalpel. DNA was extracted and various 16S rDNA hypervariable regions were PCR amplified.
Amplicons were end-repaired in preparation for blunt-end ligation to the Ion Xpress Barcode Adapters to enable sample identification. Following this, the barcoded libraries were purified using and quantified. Libraries were diluted to a concentration of 10 pM and combined in equimolar amounts for template preparation using the Ion 520 & Ion 530 Chef Kit.
STELLENBOSCH UNIVERSITY
ena-EXPERIMENT-STELLENBOSCH UNIVERSITY-23-01-2019-08:18:15:788-21