GSM1701091: NoDice(4-25) cells expressing N-terminally truncated human Dicer F1; Homo sapiens; ncRNA-Seq
Total RNA was isolated using TRIzol and small RNAs (<200 nt) purified using the mirVana Kit (Ambion) All small RNA input was processed with the TruSeq Small RNA Sample Preparation Kit (Illumina). Adapter-ligated RNA was reverse transcribed using SuperScript III (Life Technologies) and amplified using GoTaq green PCR master mix (Promega) with TruSeq 3′ indices.  DNA bands corresponding to ~145 bp were isolated by gel electrophoresis on 10% polyacrylamide gels.
GEO
GSM1701091
GEO Accession
GSM1701091