The Tsk2/+ mouse fibrotic phenotype is due to a gain-of-function mutation in the PIIINP segment of the Col3a1 gene
Total RNA was prepared from three WT and four Tsk2/+ mice skin biopsies using Qiagen RNeasy Fibrous Tissue Mini Kit. The RNA integrity (RIN) was determined by Agilent Bioanalyzer Nano chip. All samples used for this study had RIN scores of 7 or greater. RNA-seq sequencing libraries were prepared for the seven samples using NuGEN Ovation RNA-Seq System (NuGen, San Carlos, CA). Libraries were multiplexed and sequenced on an Illumina HiSeq 2000 platform to obtain 16.7-50.9 million 50 bp paired-end reads per sample. The raw reads were aligned to the reference mouse genome (MM9 assembly) using Tophat software with default parameters.
Geisel School of Medicine at Dartmouth
2214Tsk2