miRNA-seq of Homo sapiens: PMBC
Total RNA was extracted by Trizol reagent (Invitrogen) and determined by Bioanalyzer 4200 (Agilent, Santa Clara, CA, USA). Then, the next-generation libraries of mRNA were prepared using VAHTS mRNA-seq v2 Library Prep Kit for Illumina (Vazyme, Nanjing, China). The Library quality was determined by Bioanalyzer 4200 (Agilent, Santa Clara, CA, USA). Then the miRNA-seq libraries was run on an Illumina HiSeq 2500 next generation sequencer using Single-read 50-bp sequencing.
SUB6699142
P1