GSM1617490: 10pg_2; Mus musculus; RNA-Seq
Total RNA was extracted from mouse embryonic day 14 spinal cords using the RNeasy Mini Kit (Qiagen). DNA was removed with the TURBO DNA-free kit (Ambion) cDNAs were first amplified using a double-random priming strategy followed by library construction with the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB). Briefly, total RNAs was reverse-transcribed with an oligonucleotide used previously for whole genome amplification [multiple annealing and looping-based amplification cycles (MALBAC)] containing a 3' random octamer and a 5' adapter sequence. The same oligonucleotide was used for one round of second strand synthesis generating cDNA fragments that harbor the adapter sequence in a reverse-complementary manner at both ends. These fragments are then amplified by PCR using adapter oligonucleotides to produce sufficient cDNA amounts for generating high-throughput sequencing libraries.
GEO
GSM1617490
GEO Accession
GSM1617490