GSM3538863: VilCreER APCfl/fl [DVR18151217_155_S2]; Mus musculus; RNA-Seq
1cm of proximal intestine was collected and dissociated using Precyllis homogeniser in b-ME in RLT buffer.  RNA was extracted using Qiagen RNA extreatcion kit following manufacturers instructions. Libraries for cluster generation and DNA sequencing were prepared following a previously described method (Fisher et al. 2011) using TruSeq RNA sample prep kit v2 (Illumina) The libraries were run on Illumina Next Seq using the High Output 75 cycles kit (2x36 cycles, paired end reads, single index)
GEO
GSM3538863
GEO Accession
GSM3538863