454 pyrosequencing of oil sands composite tailings: 14-16m depth
DNA was extracted from ~5g of composite tailings using a PowerSoils DNA Issolation kit with low biomass modifications.  Samples were submitted for 454 pyrosequencing to MR DNA Next Generation Sequencing and Bioinformatics Services (Shallowater, TX, USA).  PCR was performed using the universal Eubacterial primers 27F and 530R. Samples were subjected to the following conditions: 28 cycles of 94°C (30 s), 53°C (40 s) and 72°C (60 s) with a final elongation step at 72°C (300 s).
SUB1185645
CT_14-16m