GSM2665633: 2-SD-T50nM; Homo sapiens; RNA-Seq
Total RNA was extracted from treated and none treated cells. Sequencing libraries were prepared according to the Illumina TruSeq Stranded mRNA Sample Prep Kit (according to manufacturer’s protocol) (Illumina, San Diego, CA). A 2 × 101 bp pairedend sequencing was performed on the HiSeq2000 instrument, using half a lane per sample, to produce on average 80 million read pairs per sample (160 million sequences) with an average insert length of 130 bp. Trimmomatic,52 Tophat2,53 Picard suite (http://www.broadinstittute.github.io/picard), RNA-SeQC,54 and in-house metrics were used to evaluate data quality.
GEO
GSM2665633
GEO Accession
GSM2665633