RNA-seq of diploid Arabidopsis: central cell
All transgenic plants were grown under the long-day condition (light/dark cycle of 16/8 hours) at 22C. Flowers at stage 12 were tagged and emasculated, and the emasculated plants were grown for another day. The ovules were dissected from pistils and soaked in protoplast enzyme solution for 15 min. The egg cell, central cell, and synergid cell labeled by nucleus-localized GFP were carefully dissected and harvested using a microcapillary pipette that is installed in a micromanipulator under an inverted dissecting microscope. Single-cell RNA-seq libraries were constructed using a modified protocol as previously described (Islam, 2014, Nature Methods)
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