GSM4278331: Non-wheezer sample 23; Homo sapiens; RNA-Seq
AMs were bead sorted and immediately lysed in TRIZol reagent. Lysates were mixed 5:1 with chloroform and centrifuged at 10 000 g for 10 minutes at 4oC to obtain RNA in top aqueous layers. Aqueous layers were then mixed with an equal volume of 70 % ethanol and RNA extracted using the RNeasy Mini Kit from QIAGEN with on-column DNase digestion, as per manufacturer's instructions. 200 ng RNA per sample was polyA-purified and converted to cDNA sequencing libraries using the Illumina TruSeq Stranded mRNA v2 kit, with unique indexes per sample to allow multiplexing.
GEO
GSM4278331
GEO Accession
GSM4278331