T2C for 30 minutes (ApoI) replicate 1
T2C was performed in duplicates as described ( Kolovos et al., 2014). In brief, ~10 million HUVEC nuclei were isolated at 0, 30 min post-stimulation, following cross-linking in 1% paraformaldehyde (10 min, 20°C; Electron Microscopy Science), chromatin was cut using ApoI or HindIII (New England Biolabs), and ligated in dilution under conditions that allowed most nuclei to remain intact.
Erasmus MC
T2C for 30 minutes (ApoI) replicate 1