GSM4333119: Zebrafish Wt brain, 5 days post fertilization; Danio rerio; RNA-Seq
Single cell RNA seq using Dropseq - 3' bead based enrichment technique, using SMART synthesis (Macosko et al, 2015 doi: 10.1016/j.cell.2015.05.002) Libraries were prepared according to Drop-seq methods (Macosko et al, 2015 doi: 10.1016/j.cell.2015.05.002). After cDNA tagmenation, PCR amplification was performed using Illumina primers (N701,N702, A501). The purified cDNA fragments of ~600 bp were captured on an Illumina flow cell for cluster generation. Libraries were sequenced on Illumina NextSeq 500 following the manufacturer's protocols.
GEO
GSM4333119
GEO Accession
GSM4333119