RNA-seq study of vitis vinifera under treatment and control samples, in different time-points
Inflorescences of Vitis Vinifera L. (Thompson Seedless cultivar) were collected from three different experimental conditions: vines sprayed with gibberellic acid (10ppm+12.5ppm+12.5ppm at 20%, 50% and 100% bloom), shaded vines (90%of PAR reduction since 50% bloom) and control (untreated vines). Inflorescences were collected at 3, 5 and 7 days after 100% bloom,deprived from the rachis and frozen in liquid nitrogen. RNA from the powdered samples was extracted and purified using the RNeasy Plant RNA Extraction Kit and RNase-Free DNase Set (Quiagen) following manufacturer’s instructions with one modification, the extraction buffer used. The samples were sequenced in an illumina HiSeq 2000, Sequencing of 21 samples in three 100bp PE channels.
CIFC-Instituto de Investigacao CientificaC
RNA vitis C1T3