Mus musculus Sleeping beauty forward genetic screen Transcriptome OSX380
RNA from 14 Sleeping Beauty mutagenized osteosarcoma (OS) samples was extracted using an RNA Midi extraction kit (Qiagen) and then quality tested using a Bioanalyzer 2100 (Agilent Technologies).  The RNA was converted to sequence libraries using the Truseq RNA Sample Preparation Kit (Illumnia Inc.,).   Lastly, each library was size selected, and the selection process was validated and quantified by capillary electrophoresis and qPCR, respectively.  Sequencing was accomplished on the HiSeq 2000 (Illumina Inc.), with the goal of generating a minimum of 70 million paired-end 50 bp reads. Sequences were mapped to a modified mm9 genome where En9 gene is masked and the sleeping beauty transposon is added as chrSB.
University of Minnesota Masonic Cancer Center
OSX380 RNASeq