GSM4175842: RNAseq from sorted GFP-pos subpopulation of ESC with Zscan4c promoter reporter driving expression of GFP; Mus musculus; RNA-Seq
Total RNA was extracted with TRIZOL and mRNA was purified using Oligo-dT Dynabeads mRNA purification kit (Invitrogen). mRNA was fragmented using 10X Fragmentation reagent (Thermo Fisher Scientific) and subjected to first strand synthesis with random hexamers and Superscript II (Invitrogen), and second strand synthesis with RNaseH (Invitrogen) and E. coli DNA polymerase I (NEB). Sequencing libraries were prepared starting from ~30ng polyA enriched cDNA with NEBNext Multiplex Oligos for Illumina kit.
GEO
GSM4175842
GEO Accession
GSM4175842