16S rRNA gene amplicons of soil layer above basaltic rock aquifer
150 g  of soil and vegetation samples were homogenised and DNA extracted from 200g using the PureLink Microbiome DNA purification Kit (Invitrogen). Partial 16S rRNA gene amplicons were generated with the F515/R806 primers. Libraries were prepared using Ilumina Nextera XT v2 barcodes and sequenced on an Illumina MiSeq.
SUB7775769
GVS8