16s rDNA sequencing of environmental samples: enriched DAMO culture
DNAs were used for PCR with the modified sequencing primers 341b4_F/806_R. The PCR products were purified with the SanPrep Column DNA Gel Extraction Kit (Sangon, China) and quantified with a Qubit 2.0 fluorometer (Invitrogen, USA). The amplicons from different samples were mixed in equimolar amounts and sequenced on the Illumina MiSeq platform with the MiSeq Reagent Kit v3 (Illumina, USA).
SUB6247053
16S14461AH