GSM3927374: H3K36me3 60mins after rapamycin IP; Saccharomyces cerevisiae BY4741; ChIP-Seq
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. 10 uL of anti-H3K4me3 (Millipore, 05-745R) or anti-H3K36me3 (abcam, ab9050) were used for precipitation 500 ng of RNA was used for library preparation with the Lexogen 3' end RNA-seq library prep kit.
GEO
GSM3927374