RNA-seq of Tasmanian Devil (Sarcophilus harrisii) : PBMCs from adult female with devil facial tumour disease untreated control
As per Patchett, A. et al 2015 Developmental & Comparative Immunology, "RNA from Tasmanian devil MNCs was re-suspended in nuclease-free water at 100 ng/_l. Samples were submitted to the Australian Genome Research Facility (AGRF) for integrity analysis and mRNA sequencing. RNA integrity was analysed using the 2100 BioAnalyser (Agilent Technologies, Santa Clara, USA). An RNA integrity number (RIN) of greater than 8 was required for mRNA sequencing. Nine single end mRNA samples were sequenced on the Illumina Hiseq-2000 platform (Illumina, San Diego, USA)."
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C2EVDACXX_CTTGTA_L003_R1