Illumina HiSeq 1500 sequencing of SAMD00089617
R. irregularis DAOM181602 was incubated with Daucus carota hairy root on M medium at 28????C for 42 days. 10 runner hyphae (ca. 1-2 mm for each) were dissected from branched absorbing structures and sampled. RNA was extracted by using NucleoSpin RNA XS kit (MACGEREY-NAGEL) with RNase-Free DNase Set (Qiagen). RNA was reverse-transcribed and amplified by using SMART-Seq v4 kit. cDNA quality and quantity were checked by using Agilent 2100 Bioanalyzer. cDNA was sheared by using Coveris S2 system and transformed to illumina libraries by using Low imput library prep kit (Chlontec). After the quality and quantity were confirmed by using Agilent 2100 Bioanalyzer and KAPA Library quantification kit, the libraries were sequenced by HiSeq 1500.
NIBB
DRX093145