GSM4175075: PTL sample83; Homo sapiens; RNA-Seq
Human placentae were obtained within one hour of delivery with signed informed consent under the protocols approved by the University of Iowa Institutional Review Board (201411731). Placenta decidual tissue samples were macroscopically isolated from the maternal-facing surface of the placenta. Samples were cut into small pieces and placed in RNAlater® solution (Applied Biosystems, Foster City, CA).  Biopsies were obtained after written informed consent, according to the protocol approved by the University of Iowa and in accordance with the Department of Health and Human Services regulations at 45 CFR 46. Total RNA was extracted from each tissue using the TRIzol reagent (Invitrogen, Carlsbad, CA) according to the manufacturer's instruction and stored at -80˚C until used RNA-seq libraries were prepared using TruSeq RNA Sample Prep Kit v2 (Illumina) and sequenced on an Illumina HiSeq 2000 to produce 27-49 million 2x100nt pair-end reads per sample.
GEO
GSM4175075
GEO Accession
GSM4175075