GSM4174674: KO-696; Mus musculus; RNA-Seq
Epididymides were harvested and teased apart in cold phosphate-buffered saline (PBS) and  rinsed in two changes of PBS to remove sperm. Samples were  homogenised using a mortar and pestle in TRIzol Reagent (Invitrogen, Carlsbad, CA, USA), according to manufacturer instructions. Total RNA was treated with DNase using  TURBO DNA-free kit (Invitrogen) and  purified using  Zymo RNA Clean & Concentrator-25 kit (Zymo Research Corporation, Irvine, CA, USA), according to manufacturer instructions. Libraries were constructed using 2ug purified RNA and the Illumina TruSeq Stranded mRNA kit (Illumina, San Diego, CA, USA) as per manufacturer instructions. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO
GSM4174674
GEO Accession
GSM4174674