GSM2904899: xPap_Mex67AA_ip_15_1; Saccharomyces cerevisiae; RNA-Seq
RNA was isolated using hot acid phenol method. For 4tU IP, 4tU residues in total RNA were biotinylated using MTSEA-biotinXX; free MTSEA-biotinXX removed and biotin-labeled RNA then purified on streptavidin beads, eluted from the column using beta-mercaptoethanol and RNA purified. An aliquot of purified RNA samples was 3' polyadenylated using E. coli poly(A) polymerase and ribo-depleted. Libraries were prepared using Lexogen QuantSeq REV kit (Lexogen GmbH, Vienna, Austria).
GEO
GSM2904899
GEO Accession
GSM2904899