Draft genome of Aeromonas hydrophila strain F2S2-1
Library preparation was performed at Genotypic Technology’s Genomics facility following NEXTFlex DNA library protocol outlined in “NEXTFlexDNA sample preparation guide (Cat # 5140-02).In brief, genomic DNA was sheared to generate fragments of approximately 200-500bp in a Covaris microTube with the S220 system (Covaris, Inc., Woburn, MA, USA). The fragment size distribution was checked using AgilentD1000Tape station(Agilent Technologies) according to the manufacturer’s instructions. The resulting fragmented DNA was cleaned up using HighPrep beads (MagBio Genomics, Inc, Gaithersburg, Maryland). These fragments were subjected to end-repair, A-tailing, and ligation of the Illumina multiplexing adaptors using the NEXTFlex DNA Sequencing kit as per the manufacturer’s instruction. The resulting ligated DNA was cleaned up using HighPrep beads (MagBio Genomics, Inc, Gaithersburg, Maryland. These adapter ligated fragments were subjected to 10 rounds of PCR (denaturation at 98˚C for 2 min, cycling (98˚C for 30sec, 65˚C for 30sec and 72˚C for 1 min) and final extension at 72˚C for 5 min) using primers provided in the NEXTFlex DNA Sequencing kit. The PCR products were purified using HighPrep beads. Quantification and size distribution of the prepared libraries were determined using Qubit flourometer (Table 1) andthe Agilent D1000 Tape Station DNA Kit (Agilent Technologies) respectively according to the manufacturer’s instructions.
Syngene International Ltd
Aeromonas hydrophila strain F2S2-1