SA_isoseq1_s3
Ae. arabicum total RNA was extracted using the CTAB protocol (Graeber et al., 2011).RNA quality was analyzed using the Plant RNA Nano assay of a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA).Library preparation and size selection was done as described by Cartolano et al., 2016.The large-scale amplified cDNAs were pooled and column purified (Qiagen PCR Purification Kit, Qiagen, The Netherlands),assessed on Agilent Bioanalyser DNA12000 chip and then three separate size ranges were fractionated on SAGEelf (SAGE Bioscience).
SUB7616835
2083.F3.E02