Altered Gut Microbiome in Patients with Phenylketonuria
Library was constructed using primers fusion. Microbial DNA was isolated from 200–300 mg fecal samples using the QIAamp Fast DNA Stool Mini Kit (Qiagen, Valencia, CA) following the manufacture instructions. Gut microbial community was determined based on partial 16S rRNA gene (V4 region) sequences directly amplified using bacterial/archaeal primer 515F/806R. Barcodes of 12 bases were added to the 5’ -end of the reverse primers. Ion OneTouch™ 2 System and Ion PGM™ Template OT2 400 Kit were used for library preparation and the sequencing was performed using Ion PGM™ Sequencing 400 kit on Ion PGM™ System using Ion 314™ Chip v2.    The seqs1.fastq file contain the following barcodes with their respective host:  #SampleID BarcodeSequence Description  c1 ATCACCAGGTGT Control  c2 ACCAGTGACTCA Control  c3 ATCGCACAGTAA Control  c4 GTCGTGTAGCCT Control  pku1 TCCCTTGTCTCC PKU  pku2 ACGAGACTGATT PKU  pku3 TGCATACACTGG PKU    The seqs2_cat.fastq file contain the following barcodes with their respective host:  #SampleID BarcodeSequence Description  c12 AGTCGAACGAGG Control  c13 ACCAGTGACTCA Control  c14 GAATACCAAGTC Control  c15 GTAGATCGTGTA Control  c5 GTCGTGTAGCCT Control  c6 AGCGGAGGTTAG Control  c7 ATCCTTTGGTTC Control  c8 TACAGCGCATAC Control  c9 ACCGGTATGTAC Control  cf1 TAACGTGTGTGC Control  cf9 CATTATGGCGTG Control  p4 TCCCTTGTCTCC PKU  p5 ACGAGACTGATT PKU  p6 GCTGTACGGATT PKU  p7 ATCACCAGGTGT PKU  p8 TGGTCAACGATA PKU  p9 ATCGCACAGTAA PKU
Federal University of Pampa
PKU