GSM2829924: mir845 2n rep1; Arabidopsis thaliana; ncRNA-Seq
Flowers were harvested in a beaker, covered with 9% sucrose solution and shaken vigorously for 5min to release pollen grains into the solution. The subsequent centrifugation- and filtering-steps were carried out in 9% sucrose solution. Total RNA was extracted using Trizol reagent (Ambion/ Life Technologies, USA) and glass beads (1.25-1.55 mm; Carl Roth). 10 μg of total RNA were run on a 14% TBE UREA Polyacrylamide gel for size selection. Gel slices containing RNA in the range of 17- to 25-nt were purified following the Illumina TrueSeq small RNA protocol for gel extraction. Libraries were constructed using the Illumina TruSeq Small RNA library preparation kit according to the manufacturer's instructions.
GEO
GSM2829924
GEO Accession
GSM2829924