RNA-Seq of Linum usitatissimum: intrusively growing phloem fibers
Intrusively growing phloem fibers (iFIB) were isolated by laser microdissection from longitudinal sections of frozen stem (1st cm from the apex) obtained by cryosectioning. Total RNA from the iFIB sample (about 500 microdissections obtained from 30 plants) was isolated using RNAqueous-Micro Kit RNA Isolation from Micro-Scale and LCM Samples (Ambion) and processed with Ultra II Directional RNA Library Prep Kit for Illumina + plant rRNA depletion. Raw reads from run were filtered to remove adapter sequences, contamination and low-quality reads.
SUB5185332
Lus_iFIBa_1