GSM1517812: WT_IC_RC12h.2011_11_30; Aspergillus fumigatus; ChIP-Seq
After reverse crosslinking, samples were treated with 2.5 mg of RNase A and incubated for 30 minutes at room temperature.  DNA was extracted either by using PCR purification kit (Qiagen) following the high pH protocol, or EtOH precipitation, with 50 mL as final volume.  5mL of sample was assayed on Qubit using high sensitivity dsDNA kit (Invitrogen).  To check sonication, 10μL of IC was run on E-gel EX 2% agarose gel (Invitrogen) and fragment size ranged from 1kb to 100bp fragmented ends were repaired, an adenine molecule was added to the repaired end, PAGE-purified adapters were added to the overhang, and the mixture amplified with primer 1.0 and primer 2.0 with an individual index tag to allow for multiplex of samples in a single lane. Samples were gel purified to obtain a size range between 200-400bp.  Libraries were validated by real time PCR, concentration was determined with Qubit (Invitrogen) and integrity was checked with an Agilent Bioanalyzer. 76 bp paired end sequencing and four ChIP samples were indexed per lane on the Illumina HiSeq 2000.
GEO
GSM1517812
GEO Accession
GSM1517812