GSM4332411: VadR, rep1; Vibrio cholerae C6706; RNA-Seq
RNA was isolated using Tri-reagent according to standard protocols. Prepared RNA was DNase digested and cleaned up using standard nucleic acid precipitation procedures. Depletion of ribosomal RNA was performed using Ribo-Zero kits (Epicentre) for Gram-negative bacteria. Integrity of the prepared RNA was tested using a Bioanalyzer. Directional cDNA libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB E7760) according to the manufacturer's instructions. Quality of the prepared libraries was tested using a Bioanalyzer.
GEO
GSM4332411
GEO Accession
GSM4332411