GSM2667845: at-endosperm-BS_replicate1_col_wt_x_col_wt; Arabidopsis thaliana; Bisulfite-Seq
Genomic DNA of INTACT-purified endosperm nuclei from 150-300 mg siliques was purified using the MagJET Plant Genomic DNA kit (Thermo Fisher Scientific), eluted in 80 μl of elution buffer, and sonicated for 30 cycles (30s on, 30s off at high power) using a Bioruptor (Diagenode). The bisulfite conversion was performed using the EpiTect Fast DNA Bisulfite Kit (Qiagen) during the library preparation. Bisulfite libraries were generated with the Ovation Ultralow Methyl‐Seq Library Systems (NuGEN) following the manufacturer's protocol using 10 ng of starting DNA.
GEO
GSM2667845
GEO Accession
GSM2667845