Day 3 surface survival, biological replicate 1
Day 3 surface survival of A. baumannii. Transposon mutants were collected by scraping cells off a Q-tray containing tryptic soy agar. Cells were frozen at -80°C for Tn-seq library preparation. Genomic DNA was extracted using a DNeasy Blood and Tissue Kit (Qiagen). Tn-seq libraries were prepared using the terminal deoxynucleotidyl transferase (TdT) method as previously described (1, 2). See reference 2 for primer sequences used in this study. Sequencing was carried out on an Illumina Miseq. 1. Klein BA, Tenorio EL, Lazinski DW, Camilli A, Duncan MJ, Hu LT. 2012. Identification of essential genes of the periodontal pathogen Porphyromonas gingivalis. BMC Genomics 13:578. 2. Gallagher LA, Ramage E, Weiss EJ, Radey M, Hayden HS, Held KG, Huse HK, Zurawski DV, Brittnacher MJ, Manoil C. 2015. Resources for Genetic and Genomic Analysis of Emerging Pathogen Acinetobacter baumannii. Journal of bacteriology 197:2027-2035.
University of Washington
Day 3, biological replicate 1