16S H2O Cranberry Creek
Samples stored in DNA storage buffer on 0.2m polycarbonate filters, followed by DNA extraction with EZNA Kit (See Github for exact protocols). DNA was amplified via PCR for V6-V8 region of 16S rRNA gene (primers: B969F and BA1406R). Samples were cleaned with Agencourt AMPure XP PCR purification kit. Illumina kit used was 2x300bp v3 chemistry with 10% PhiX spike.
SUB5358937
H2O_045_518_719