Pyrosequencing of nitrous oxide reductase gene nosZI from marine sediments prior to incubation
DNA was extracted from sediments using the Fast DNA Spin Kit for Soil (MP Biomedicals, Santa Ana, USA). A two-step PCR was performed to amplify the nitrous oxide reductase gene nosZI (primers: 1840F/2090R, Henry et al., 2006) and introduce the barcode, sequencing key and adapter. Pyrosequencing was performed by Genoscreen (Lille, France) on a Roche’s 454 FLX Genome Sequencer using Titanium chemistry. Sequence data was initially screened and de-multiplexed using QIIME (version 1.8.0, Caporaso et al., 2010) with the default parameters.
Swedish University of Agricultural Sciences
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