GSM2667725: at-seed-sRNA_replicate1_ler_wt_x_col_wt; Arabidopsis thaliana; ncRNA-Seq
For each genotype 1000 seeds at 6-7 DAP were collected in duplicates in RNA later (Sigma–Aldrich) and homogenized (Silamat S5) using glass beads. Total RNAs of seeds were extracted using mirVana miRNA isolation kit (Ambion/Life Technologies) and sRNAs were isolated by FDF-PAGE. SRNA libraries were generated using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina and the libraries were sequenced with an Illumina HiSeq2500 in 50-bp single-end fashion.
GEO
GSM2667725
GEO Accession
GSM2667725