GSM2480407: Over-2; Homo sapiens; ChIP-Seq
Empty vector control and HA-tagged ZNF322A expressing H460 cells (1 × 10^7 cells) were cross-linked with 1% formaldehyde, followed by preparation of nuclear lysates using Magna ChIP protein G Kit (Millipore, Billerica, MA, USA) according to the protocols provided by the manufacturer. Nuclear lysates were sonicated for shearing crosslinked DNA to around 200~300 bps using Covaris-S2 machine (Covaris Inc., Woburn, MA, USA). Chromatin was immunoprecipitated with anti-HA antibody (GeneTex, GTX29110). Purified chromatin-immunoprecipitated DNA was subjected to preparation of fragment libraries using 5500 SOLiD Fragment Library Core Kit (Applied Biosystems, Foster City, CA, USA) according to the protocols provided by the manufacturer.
GEO
GSM2480407
GEO Accession
GSM2480407