GSM4174996: HR_2; Rattus norvegicus; RNA-Seq
The rats were sacrificed in adulthood (bHR/bLR=P160-P167, bIR=P126-134) by rapid decapitation and the whole hippocampus was extracted on ice, rapidly frozen, and stored at -80 degrees C.  Nucleotides were extracted using Qiagen AllPrep DNA RNA miRNA Universal Kit 50. Extracted RNA was evaluated for total concentration and quality using a Nanodrop spectrophotometer (concentration range 285-432 ng/ul, 260/280 ratio range 1.61-1.80). At the sequencing core, the RNA was re-assessed for quality using the TapeStation automated sample processing system (Agilent, Santa Clara, CA) and only samples with RNA integrity numbers (RINs) of  >8 were included in the analysis. The cDNA library was constructed using 0.1-3ug of total RNA and the Illumina TruSeq Stranded mRNA Library Preparation kit (Catalog #s RS-122-2101, RS-122-2102) (Illumina, San Diego, CA).   The final cDNA libraries were checked for quality once again by TapeStation (Agilent) as well as qPCR through the use of Kapa's library quantification kit for Illumina Sequencing platforms (catalog # KK4835, Kapa Biosystems,Wilmington MA). The samples were clustered on a cBot automated cluster generation system (Illumina) for clonal amplification. The samples were then hybridized to the slide (“flow cell”) of a HiSeq 2000 (Illumina) with 6.66 samples per lane and underwent a 100 cycle paired end run in High Output mode using version 3 reagents.
GEO
GSM4174996
GEO Accession
GSM4174996