CK
Following the manufacturers instructions, 0.5 g fresh soil was used for soil DNA extraction with a FastDNA SPIN Kit (MP Biomedicals, Santa Ana CA, USA).The amplification of 16S rRNA for 454 sequencing was performed according to the method described by Shen et al. (2013). Briefly, F515 (50-GTGCCAGCMGCCGCGG-30) and R907 (50- CCGTCAATTCMTTTRAGTTT-30) were used as primers to amplify V4-V5 regions of bacterial 16S rRNA genes (Sun et al. 2013).
SUB6257400
G1bR1