GSM1938071: 1009Fru1; Streptococcus mutans; RNA-Seq
Total RNA was extracted from bacterial cells using the RNeasy Mini kit (Qiagen). To remove 16S and 23S rRNAs, 10 µg of total RNA was treated using the MICROBExpressTM Bacterial mRNA Enrichment Kit cDNA libraries were created from the enriched mRNA samples using the NEBNext Ultra Directional RNA Library Prep kit for Illumina and NEBNext multiplex Oligos for Illumina (New England Biolabs, Ipswich, MA) following instructions from the supplier. RNA-Seq: single-ended short reads were obtained from a set of bar-coded cDNA libraries
GEO
GSM1938071
GEO Accession
GSM1938071