Illumina HiSeq 2000 sequencing; RNA-seq analysis of C. elegans mina-1 mutants
RNA-seq analysis of C. elegans mina-1 mutants
All C. elegans strains were maintained and raised at 20°C on NGM agar seeded with Escherichia coli OP50 (Brenner, 1974). RNA extraction was done using TRIzol reagent (Life Technologies) in accordance with the manufacturer's protocol and additionally purified by a solution of acidic phenol:chloroform (5:1 v/v) pH 4.5 (Ambion) followed by a second chloroform extraction to obtain high-grade RNA for sequencing. Finally, DNA traces were degraded by DNAse I (Ambion). Random primed cDNA library was generated from each sample by GATC Biotech.
University Children's Hospital Basel, University of Basel, Spitalstrasse 33, 4056 Basel, Switzerland
E-MTAB-7389:mina-1_YA_2_s
Experimental Factor: genotype
mina-1/C41G7.3(ok1521) I
Experimental Factor: strain
VC1054