Amplicon of Oncorhyncus mykiss: gills-RTGI2
"Whole tissue samples were collected from rainbow trout and homogenized with tungsten beads. Whole genomic DNA was extracted, containing both bacterial and host DNA. 16S primers A17F and 519R were used to amplify the V1-V3 area of the 16S rRNA region of the DNA, creating an amplicon about 500 bp in size. Amplicons were gel extracted, then 454 pyrosequenced."
University of New Mexico
Gills2