Total RNA was extracted using the RNeasy kit (Qiagen) according to the manufacturer's instructions. The RNA integrity number (RIN) was quantify on the Agilent 2100 bioanalyzer. cDNA libraries were prepared with TruSeq stranded mRNA library prep Kit (# RS-122-2101, Illumina) using 500ng of total RNA.
SUB2555757
PC3_B_pCMV_empty_sirna_scramble_EtOH