GSM3538985: clonal iPSC line with 109 CAG repeats_706-2; Homo sapiens; RNA-Seq
After medium removal, iPS cells were washed once with PBS and subsequently covered with 1 mL of RNAzol RT RNA Isolation Reagent (GeneCopoeia, Inc.), scraped and frozen in -80°C. Upon thaw, total RNA isolation was performed according to the manufacturer's protocol with 75% ethanol, isopropanol, and RNase-free water. Isolated total RNA was then treated with TURBO DNase (ambion) and purified with QIAquick® Nucleotide Removal Kit (QIAGEN). Each reaction was performed in PCR probes and contained 10 μg of RNA sample, 10 x TURBO Buffer, RNaseOUT (Invitrogen), DNase TURBO and RNase- RNA libraries were prepared for sequencing using standard Illumina protocols
GEO
GSM3538985
GEO Accession
GSM3538985