GSM3684805: ARh2_Ref3; Thioalkalivibrio thiocyanoxidans ARh2; RNA-Seq
The frozen cell pellets were homogenized with a mortar and a pestle before being resuspended in QIAzol Lysis Reagent (Qiagen, Germany). Total RNA was extracted and purified with the RNeasy kit (Qiagen) following the manufacturer's instructions. The purification step comprised a DNase treatment using the RNase-free DNase kit (Qiagen). The concentration was quantified with the NanoDrop ND2000 (Thermo Fisher Scientiﬁc, USA) and the integrity of the RNA was checked on the 2200 TapeStation with Agilent RNA ScreenTapes (Agilent Technologies, The Netherlands). Ribosomal RNA (rRNA) was removed by the Illumina Ribo-Zero rRNA Removal Kit (Bacteria) (Illumina, USA). Bar-coded RNA libraries were prepared using the Ion Total RNA-Seq kit v2 and the Ion Xpress RNA-Seq barcoding kit according to the supplier's instructions (Thermo Fisher Scientiﬁc). Ion Proton platform with an Ion PI Chip v3 (Thermo Fisher Scientiﬁc)
GEO
GSM3684805
GEO Accession
GSM3684805