RNA-Seq of Neovison vison: feces
The nucleic acid samples were depleted for DNA using DNase I (Thermofischer) and the enzyme kit Superscript III (Invitrogen) was used for generating single-stranded cDNA with random hexamer primers. Second strand cDNA was generated using the NEBNext mRNA second strand synthesis kit (New England Biolabs). Sequencing libraries were made using the Nextera-XT DNA library Preparation kit and sequenced on a MiSeq machine with Reagent Kit v3 600 bp
SUB6430450
D28-3