Fungal ITS region amplicon sequencing of Spartina alterniflora rhizosphere: control
Genomic DNA from soil samples was extracted using a MoBio PowerSoil DNA extraction kit. Amplicons were prepared from total DNA by PCR targeting the fungal ITS region (forward: ITS1F: CTTGGTCATTTAGAGGAAGTAA and reverse: 58A2R: CTGCGTTCTTCATCGAT) (Martin & Rygiewicz, 2005).  PCR amplicons were quantified using PicoGreen, normalized at 1 ng l-1, pooled together and sent for sequencing on an Illumina MiSeq (paired-end 2x250bp) at the McGill University and Genome Quebec Innovation Center (Montral, Canada).
SUB5194387
Fungi.3.Sal.C3