Tn-Seq sample from Bacillus subtilis 168 harvested 5 hours post-starvation (T5)
Tn-seq sample of Bacillus subtilis 5 hours post-starvation (T5). A magellan6 transposon library was grown in Difco Sporulation Medium to the onset of starvation, then allowed to sporulate for 5 hours. A sample was harvested, heated at 80C for 20min and germinated on LB agar. The germinated colonies were pooled and genomic DNA extracted.  The library was constructed by MmeI digestion of genomic DNA and adapter ligation, followed by a 16 cycle PCR amplification to select for transposon insertion sites. This sample was sequenced on the Illumina HiSeq 2500 platform.
Harvard University
T5