GSM3928305: MAN; Bacillus methanolicus; RNA-Seq
B. methanolicus cells were harvested in the middle of the exponential growth phase followed by total RNA isolation individually for each cultivation condition using the NucleoSpin RNA isolation Kit (Machery-Nagel, Düren, Germany) and the RNase-free DNase set (Qiagen, Hilden, Germany). Isolated RNA samples (triplicate samples per condition) were pooled in equal parts and the total RNA was subsequently used for the cDNA library preparation. The library was prepared and sequenced on a single flow cell of a MiSeq Desktop Sequencer system (Illumina, San Diego, CA, USA) in paired-end mode following a protocol that allowed for the analysis of the whole transcriptome  (Mentz et al., 2013).
GEO
GSM3928305
GEO Accession
GSM3928305