GSM3537562: Rosette leaf rep1; Arabidopsis thaliana; RNA-Seq
RNA extraction was conducted using TRIzol reagent (Invitrogen). Ethanol precipitation was performed overnight at -20ºC to maximize sRNA yield. RNA pretreatment was conducted using rtStar tRF&tiRNA pretreatment kit (Arraystar). Pretreated RNAs were purified by Phenol:Chloroform extraction and ethanol precipitation. Pretreated RNAs were subjected to small RNA-seq library construction using VAHTS Small RNA Library Prep Kit for Illumina (Vazyme). Purification of PCR amplified cDNAs were conducted using VAHTS DNA Clean Beads (Vazyme). tRNA-seq
GEO
GSM3537562
GEO Accession
GSM3537562