RNA-Seq of Mus musculus: adult female tumor associated macrophages isolated from CT26-Sg-Con tumors: Rep 2
For RNA sequencing, libraries were prepared using an MGIEasy mRNA kit and sequenced by a BGISEQ-500 instrument. Upon sequencing, raw FASTQ files were aligned using a HISAT aligner with default parameters. Aligned fragments were then counted and annotated using the Bowtie2 transcript database. Normalized RSEM were obtained.
Xinqiao hospitao, Chongqing,China
TAM_1-2