ChIP-Seq analysis of H3K9me3 in human hN (ES H9 derived Neurons) cells
Single read - Illumina
BI
Lib EpiLabSPL Row 1690
CHIP_PROTOCOL_BEAD_TYPE
Protein A-Sepharose 4B
CHIP_ANTIBODY
H3K9Me3
CHIP_PROTOCOL_CHROMATIN_AMOUNT
10 million cells
EXTRACTION_PROTOCOL
SDS lysis
CHIP_PROTOCOL_ANTIBODY_AMOUNT
2-15 ug
CHIP_PROTOCOL_BEAD_AMOUNT
70 ul bed volume
CHIP_PROTOCOL
Bernstein_BROAD_ENCODE_protocol
EXTRACTION_PROTOCOL_SONICATION_CYCLES
1
CHIP_ANTIBODY_CATALOG
ab8898
CHIP_ANTIBODY_PROVIDER
Abcam
CHIP_ANTIBODY_LOT
484088
EXPERIMENT_TYPE
Histone H3K9me3
EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR
Bioruptor
GEO Accession
GSM772738