GSM3381785: sRNA-seq-CK3; Camellia sinensis; miRNA-Seq
Total RNA was isolated from each frozen sample using Trizol reagent (Invitrogen, CA, USA) according to the manufacturer's protocol. The quantity and quality of the total RNA were determined using a Bioanalyzer 2100 (Agilent, CA, USA) and the RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0. Equal quantities of total RNA from C. gloeosporioides- and mock-inoculated leaves collected at four days post infection (4dpi) were used to prepare the sRNA libraries using the TruSeq Small RNA Sample Prep Kit (Illumina, San Diego, CA, USA). We performed single-end sequencing (36 bp) on an Illumina Hiseq 2500 at the LC-BIO (Hangzhou, China) using the vendor's recommended protocol.
GEO
GSM3381785
GEO Accession
GSM3381785