Bar-Seq of Homozygous deletion collection from Saccharyomyces cerevisae: N2141 treated
The uptag barcode was amplified from genomic DNAprepared from DMSO-treated and MEHP-treated yeast cells using a commonreverse oligo Rc and a variable forward oligo Fv containing a sample-specific TruSeqindex. Sequence of Rc is5AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT GTCCACGAGGTCTCT 3. Sequence of Fv is 5 CAAGCAGAAGACGGCATACGAGATNNNNNNGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTGTCGACCTGCAGCGTACG 3. Hexanucleotide NNNNNN in Fv is the sample-specific TruSeq index.PCR products were pooled and sequenced on an Illumina HISEQ with 1x51 single-end reads. Sequencing data was analyzed as described previously
SUB5195181
BYS068