NextSeq 500 paired end sequencing; RNA-seq of TGF-beta and knockdown of histone variant H2A.Z induced EMT in MDCK cells.
RNA-seq of TGF-beta and knockdown of histone variant H2A.Z induced EMT in MDCK cells.
MDCK cells were maintained in DMEM growth medium supplemented with 10% Foetal Bovine Serum (FBS) For TGF-beta treatment, wildtype MDCK were stimulated in culture by adding 5ng of TGF-beta to the medium. TGF-beta was added with every passage of cells, to maintain stimulus. Total RNA was extracted usign Qiagen RNAeasy kit following manufacturer's instructions. Libraries were constructed according to the Illumina TruSeq stranded mRNA protocol with poly-A enrichment.
Research Fellow Tremethick Lab Department of Genome Sciences The John Curtin School of Medical Research The Australian National University
E-MTAB-5628:MDCKTGFbD6_1_p
Experimental Factor: TGF-beta
stimulus
Experimental Factor: no konckdown
RNA interference