GSM4061831: NX_Lung; Mus musculus; RNA-Seq
Sorted lung myeloid cells were suspended in PBS containing 0.04% molecular biology grade non-acetylated BSA at a concentration of 1,000cells/ µL. The purity of the sorted cells was verified and the sorted cell number was verified by manual counting. Using the 10x GemCode technology single cells were captured with barcoded beads in a droplet emulsion for the RT reaction. After obtaining 10x barcoded cDNA, libraries were prepared following the standard protocol of 10X Single Cell 3' v1. The libraries were sequenced with Hi-Seq 4000 (Illumina). scRNA-Seq generated using 10x genomics Single Cell 3' Reagent Kits V1
GEO
GSM4061831
GEO Accession
GSM4061831