Thlaspi arvense transcriptome
total RNA was collected from plant tissue and purified using the Qiagen RNeasy Plant Mini Kit. Samples were quantified using fluorimetry (RiboGreen assay) and RNA integrity was analyzed using capillary electrophoresis (Agilent BioAnalyzer 2100). Polyadenylated RNA was selected using oligo dT purification and reverse transcribed to create cDNA. cDNA was fragmented, blunt ended, and ligated to the Illumina TruSeq Adaptor Index 3 (TTAGGC). The library was then size selected  and quantified using fluorimetry (PicoGreen assay).
University of Minnesota
Thlaspi arvense de novo transcriptome