RNA-Seq of plasma from patient with prostate cancer before radical prostatectomy.
Total RNA was isolated from  plasma from patient with prostate cancer before radical prostatectomy. Ion Total RNA-Seq Kit v2 was used to construct transcriptomic library. Excessive rRNA was removed by incubation of the final library with Duplex-specific nuclease and additional round of amplification by PCR.
SUB2115070
PC348R