GSM4494720: ATAC-seq FSHD iPSC control #2; Homo sapiens; ATAC-seq
cells were processed immediately for ATACseq Cells were lysed in cold lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, and 0.1% IGEPAL CA-630), and the nuclei were pelleted and resus¬pended in transposase buffer. The Tn5 (Illumina FC-121-1030), and the transposition reaction was carried out for 30 min at 37 °C. After purifica¬tion, the Nextera libraries were amplified for 12 cycles with NEBnext PCR master mix and purified with a Qiagen PCR cleanup kit.
GEO
GSM4494720
GEO Accession
GSM4494720