5' OH RNA seq of M. tuberculosis: 7 days, uninduced control
To select for 5' OH RNA, total RNA was digested with 1 U of Terminator 5-Phosphate-Dependent Exonuclease and phosphorylated with 3 u of T4 PNK. Adaptors were ligated with T4 RNA ligase 1, followed by Reverse transcription with Superscript IV, and PCR with adaptor-specific primers. See Schifano et al. (2014) Nat. Comm. PMID 24709835.
SUB4499757
VJ07