RNA-seq of Penicillium oxalicum HP7-1 in Starch culture media
mRNAs are isolated from total RNA with oligo(dT) method and then reverse transcribed to cDNA after fragmentation. cDNA fragments are purified and resolved with EB buffer for end reparation and single nucleotide A (adenine) addition. After that, the cDNA fragments are linked with adapters. Those cDNA fragments with suitable size are selected for the PCR amplification.
SUB4975811
RS_S2