GSM2829766: RNAseq_SCer_12_Async_23C_rep1; Schizosaccharomyces pombe; Saccharomyces cerevisiae; RNA-Seq
RNA-seq: Cells were counted and 10% S. pombe cells were added. Total RNA was extracated with Qiagen Rneasy columns. mRNA was selected with oligo-dT bead. cDNA was prepared based on the incorporation of dUTPs using reverse transcription. DNA was ligated to Illumina or home-made multiplexed adapters and amplified by PCR. For 3C-seq library DNA between 400-1Kb was gel purified. For ChIP Library DNA between 250-500 bp in size was gel purified.
GEO
GSM2829766
GEO Accession
GSM2829766