GSM1617325: WT T0 MNase-seq_1; Saccharomyces cerevisiae; MNase-Seq
For MNase-seq, Cells were cross-linked by 0.1% Para formaldehyde for 15 min and then treated with 0.125 M glycine. The pellets were subjected to MNase digestion as previously reported (Kaplan et al. 2008). The DNA with the size of mononucleosomes was used to construct libraries. For RNA-seq, Total RNAs were extracted as previously described with phenol/freeze method (Schmitt et al. 1990). RNA qualities were checked with the Fragment Analyzer (AATI). The DNA from Mmase and ChIP was used to construct libraries according to manufacture’s guide (Illumina). The library was sequenced using the Illumina 1G Genome Analyzer and Highseq-2000. For RNA-seq, The extracted RNAs were further processed to RNA fragmentation and construct library for Illumina sequencing with the TruSeq Stranded mRNA Sample Prep Kit - Illumina.
GEO
GSM1617325
GEO Accession
GSM1617325