GSM4061958: HCT116_LSH_KO_RRBS; Homo sapiens; Bisulfite-Seq
To prepare genomic DNA for RRBS analysis, cells were resuspended with cell lysis buffer (10mM Tris pH 7.5, 10 mM EDTA, 10 mM NaCl, 0.5% sarcosyl, 0.1 mg/mL RNase (CWBIO)) and incubated at 37°C overnight. Then, protease K was added to a final concentration of 0.2 mg/mL and incubated at 65°C for 24 hr. Genomic DNA was extracted by phenol/chloroform and ethanol precipitated. The DNA were dissolve in ddH2O.Total RNA for RNA-seq analysis was extracted from cells using RNAiso Plus Reagent (Takara) according to the manufacturer's instructions.
GEO
GSM4061958
GEO Accession
GSM4061958