GSM2829305: eWAT_pop4_HFD2; Mus musculus; RNA-Seq
blood is collected by cardiac puncture on an EDTA coated syringe,  and lysed twice. Adipose tissue is collected in PBS, and incubated in 4mg/mL collagenase II,  supplemented with 2mmol/L flavopiridol and 0.25% BSA and 5mM Cacl2 for 20 min at  room temperature under agitation, before mechanical disruption with a 10mL pipette. Cell suspension were  centrifuged for 10min at 500g and the samples are resuspended in 70μL of Facs buffer  containing anti CD16/32 (FcRIII/II). 200 cells from each sample were directly sorted in a  96 wells plate (biorad) in 4μL H2O containing 0.2% Triton X-100 (sigma) and 0.8U/mL  RNase inhibitor (clontech). Illumina hiseq libraries were prepared with 10ng amplified cDNA, using 8 cycles of PCR with Kapa library preparation chemistry kit (Kapa Biosystems).
GEO
GSM2829305
GEO Accession
GSM2829305