cow B D7 Cellulose #1
Sample collected from Cellulose culture #1 (cellulose supplementation) at day 7 time point. Cultures consisted of rumen fluid (2.2L collected from a cannulated beef cow on a forage diet) maintained as batch cultures in bioreactors under anaerobic conditions, at a temperature of 38.3 C and constant agitation (150 rpm).  Samples kept at -20C until microbial genomic DNA extractions. Microbial genomic DNA was extracted using the repeated bead beating + column protocol. PCR amplification of bacterial V1-V3 regions of 16S rRNA genes using 27F-519R primers. Sequencing using MiSeq2X300.
SUB3150570
cow B D7 Cellulose #1