GSM2907047: Nitrate replicate 3.2; Haloferax mediterranei; miRNA-Seq
microRNAs were isolated with mirVanaTM miRNA isolation kit (Ambion) following product specifications. Afterwards, the RNA samples were treated with Turbo DNase (Ambion). Size-selected RNA concentration was quantitated using the NanoDrop ND-100 Spectrophotometer (NanoDrop Technologies, Wilmington, DE) and size distribution visualized using Agilent 2200 Tapestation (Agilent Technologies, Palo Alto, CA, USA). microRNAs libraries were constructed using TruSeq Small RNA Library Prep Kit and the TruSeq Small RNA Library Prep Guide, Part #15004197 Rev. G protocol.
GEO
GSM2907047
GEO Accession
GSM2907047