H_T2_2
The coral fragment was snap-frozen with liquid nitrogen, and stored at -80 C. Total RNA was extracted using TRIzol reagent, PureLink RNA kit and PureLink DNase Invitrogen, Ca, United States) according to manufacturers instructions. RNA-seq libraries were prepared using an in-house protocol at the Weizmann Institute of Science. Briefly, the polyA fraction (mRNA) was purified from 500 ng of total RNA per sample following by fragmentation and generation of double-stranded cDNA. Then, end repair, A base addition, adapter ligation and PCR amplification steps were performed. Libraries were evaluated by Qubit (Thermo Fisher) and TapeStation (Agilent). Sequencing libraries were constructed with barcodes to allow multiplexing of 2 samples in 2 lanes.
SUB7769909
H_T2_2