GSM4125119: Zglp1KO_cloneE5_c0_n1; Mus musculus; RNA-Seq
For transcriptome analysis, total RNAs of ESCs, EpiLCs, day 4 (c0) PGCLCs (BV-sorted), c3, c4, c5, c7, and c9 cultured PGCLCs (SC-sorted) were extracted and purified using an RNeasy Micro Kit or NucleoSpin RNA XS Kit.  For each sample, 3' ends of mRNAs were enriched/amplified from 1 ng of total RNAs as the starting material as previously reported ( Nakamura, 2015, GSE63266). The cDNA library preparation for Nextseq500 (Illumina) was performed as previously reported (Nakamura, 2015, GSE63266, Ishikura, 2016, GSM2328360).
GEO
GSM4125119
GEO Accession
GSM4125119