GSM2667727: at-pollen-sRNA_col_wt_1n; Arabidopsis thaliana; ncRNA-Seq
Mature pollen was harvested by manual collection of open flowers into a 18% sucrose collection buffer placed on ice. Tubes containing the extraction buffer and open flowers were vortexed for 5 minutes and centrifuged for 3 minutes at 3,000 rpm in a centrifuge equipped with a tilt rotor. Pollen RNA was extracted with TRIzol reagent according to manufacturer instructions. Small RNA was enriched by cutting the 17-29nt small RNA fraction from a polyacrylamide gel and subsequent elution and precipitation.  Cloning of small RNAs was performed using the Illumina TruSeq RNA Sample Preparation Kit For the library preparation the Illumina TrueSeq small RNA protocol was followed and the libraries were sequenced with an Illumina HiSeq2500 in 50-bp single-end fashion.
GEO
GSM2667727
GEO Accession
GSM2667727