RNA-seq of maize ear primordia in response to drought
Approximately 50ng of mRNA was used as input for the NEBNext Ultra RNA Library Prep Kit.  This kit allowed the addition of barcoded adapters to enable multiplexed sequencing of 7-8 samples in a single lane of an Illumina HiSeq2000.  Prior to sequencing, the average size distribution of the libraries was verified on a high sensitivity bioanalyzer chip.  The libraries were diluted to 10nM and concentration was verified by qPCR using standards (KAPA Biosystems).
SUB2763561
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