hypersaline microbial mat from ESSA Area 1
Total metagenomic DNA was extracted with the commercial kit Power Biofilm DNA Isolation Kit; Mo Bio Laboratories, Carlsbad, CA, USA. Oligonucleotide primers 926F and 1392R were used to generate amplicons spanning the hypervariable regions of the 16S rRNA gene from V6 to V9. PCR products were purified using QIAquick PCR purification columns (Qiagen Inc., Valencia, CA) and pooled in equimolar concentrations. The amplicon library was sequenced on a Genome Sequencer FLX Titanium pyrosequencer (Roche, Brandford, CT) in the University of Arizona Genetic Core.
SUB3071795
ESSA-A1