GSM4174023: TGFb12h, biological rep1 [ATAC-seq]; Mus musculus; ATAC-seq
The ATAC experiments were performed as described (Buenrostro et al., 2013). NMuMG cells were either vehicle or TGFβ-treated for 2 or 12h, and then collected and treated with transposase Tn5 (Nextera DNA Library Preparation Kit, Illumina). DNA was purified using MinElute PCR Purification Kit (Qiagen). All samples were then amplified by PCR using NEBNextHigh-Fidelity 2× PCR Master Mix (New Englands Labs) with primers containing a barcode to generate libraries. DNA was again purified using MinElute PCR Purification kit and samples were sequenced using Illumina NextSeq 500 system (Illumina) with 75 bp paired-end reads
GEO
GSM4174023
GEO Accession
GSM4174023