Nosema ceranae  spores
Total RNA of N. ceranae spores was isolated using TRizol reagent (Thermo Fisher, China) on dry ice followed by reverse transcription with Maxima H Minus Reverse Transcriptase, and then purified and concentrated on a AMPure XP beads. cDNA ibraries were constructed from 50 ng total RNA with a 14 cycles PCR using the cDNA-PCR Sequencing Kit (SQK-PCS109) and PCR Barcoding Kit (SQK-PBK004) according to the manufacturer's protocol (Oxford Nanopore Technologies Ltd, UK), followed by sequencing by Biomarker Technologies (China) using PromethION platform (Oxford Nanopore Technologies Ltd, UK)
SUB7762613
NC