p2_r3_d0_bottom
DNA for 16S rRNA amplicon sequencing analysis was amplified using 12.5 L AccuStart II pCR ToughMix (QuantaBio, Beverly, MA), 1 L Golay barcode tagged 515 forward primer 22, 1 L 806 reverse primer, 9.5 L PCR water, and 1 L of template DNA. PCR conditions were: 94 C for 3 minutes, 35 cycles at 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; with a final extension of 10 min at 72 C. Amplicons were quantified using PicoGreen (Invitrogen) and pooled to equimolar amounts. A pooled final concentration of 6.75 pM was used with a 10% PhiX spike for sequencing on the Illumina MiSeq. Amplicons were sequenced on a 2x150 bp paired end MiSeq run
SUB5004546
Q.B.3.bottom.Day0