mRNA-seq of wild-type protonema, dark-grown control, biological replicate 1
Libraries for RNA-seq were prepared by following Illumina TruSeq stranded mRNA sample preparation kit according to the manufacturer’s protocol. In brief, 4 ug of total RNA were used for library construction. PolyA RNA was captured by oligo dT beads and fragmented after elution from the beads. The cDNA was synthesized by reverse transcription and followed by ligation of the barcoded Truseq adapters. The products were purified and enriched with 10 cycles of PCR to create the final library. The libraries were analyzed by using DNA analyzer to estimate the quantity and size distribution, then pooled for pair-end sequencing on Illumina HiSeq 2500.
SUB2753293
WT_D_rep1