RNA-seq was performed in MCF-7 cells which were treated with PHF8 siRNA
MCF-7 cells were treated with PHF8 siRNA (ON-TARGETplus, L-004291-01-0005, Dharmacon) and harvest after 72 hours. Total RNA was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA). The quality of total RNA was checked using the NanoDrop Spectrometer (ND-1000 Spectrophotometer, Peqlab) and the Agilent 2100 Bioanalyzer (RNA Nano Chip, Agilent). High quality RNA samples (20 μg each) were sent to Beijing Genomics Institute (BGI, Shenzhen) for cDNA libraries construction and sequencing using Illumina HiSeqTM 2000.
Tianjin Medical University
RNA-seq result for knockdown PHF8