NextSeq 500 paired end sequencing; Whole-islet RNA-sequencing analysis of human pancreas and type 2 diabetes.
Whole-islet RNA-sequencing analysis of human pancreas and type 2 diabetes.
Human primary islets were purchased from Prodo Laboratories Inc (Irvine, CA, USA)  Qiagen RNeasy microkit with on-column DNase digestion (Qiagen, Hombrechtikon, Swizerland) Illumina TruSeq Stranded mRNA Library prep kit following the manufacturer’s recommendations.
Department of Cell and Molecular Biology (CMB), Karolinska Institutet, Stockholm, Sweden.
E-MTAB-5060:HP1525301T2D_wholeislet_p
Experimental Factor: type 2 diabetes mellitus
disease