destabilization_day_50
Fungal internal transcribed spacer (ITS) was amplified using ITS5F (5?-GGAAGTAAAAGTCGTAACAAGG-3?) and ITS4R (5?-TCCTCCGCTTATTGATATGC -3?) (White et al. 1990) containing the Illumina-specific MID barcodes. Amplification was done with the PCR Thermal Cycler Multigene Optimax (Labnet, Edison, NJ, USA) using the following steps: (1) initial denaturation at 94  C for 10 min; (2) 25 cycles of further denaturation at 94  C for 45 s, annealing at 53  C for 45 s, and extension at 72  C for 1 min; (3) concluding extension at 72  C for 10 min. The PCR mixture contained 1.25 ?L DMSO.
SUB3398590
T20.3