GSM1942176: WT_pSHF_3; Mus musculus; RNA-Seq
Posterior second heart field tissue was microdissected from E9.5 timed matings. Pools of tissue were mechanically homogenized in TRIzol Reagent (Invitrogen) and then RNA was isolated in accordance to the manufacturer’s instructions. Libraries were prepared from this RNA starting with 1μg per sample and using the mRNA-seq Sample Prep Kit (Illumina) as per recommended instructions. Barcoded Libraries were prepared according to Illumina's instructions (2013) accompanying the TruSeq RNA Sample prep kit v2  (Part# RS-122-2001). Libraries were quantitated using the Agilent Bio-analyzer (model 2100) and pooled in equimolar amounts. The pooled libraries were sequenced on the HiSeq2500 in Rapid Run Mode following the manufacturer's protocols (2013).
GEO
GSM1942176
GEO Accession
GSM1942176