Illumina HiSeq 2500 paired end sequencing; Single-cell RNA-seq of dermal fibroblasts derived from healthy human donors
Single-cell RNA-seq of dermal fibroblasts derived from healthy human donors
Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122). All cells were derived from healthy donors and were nontransgenic. Reverse transcription and cDNA amplification was performed according to the SmartSeq2 protocol (Picelli et al., 2014) Library preparation was performed using an Illumina Nextera kit. We used ERCC spike ins from Life Technologies, catalogue number 4456740, at a concentration of 1:64Million. All uploaded cells have passed our QC (we have not included failed cells), we have outlined the QC process/thresholds in the accompanying publication.
EMBL-EBI & Wellcome Sanger Institute
E-MTAB-71671537448367:22259_1_55_p
Experimental Factor: individual
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