Application of Ion Torrent sequencing to the assessment of the effect of alkali ballast water treatment on microbial community diversity Sample 28P
NaOH treated ballast water samples and control ballast water samples were collected in a Great Lakes ship. DNA were extracted from the water samples, and 16S rRNA gene was amplified using 515F and 926R primers. PCR amplicon was sequenced using Ion Torrent PGM with a 400 base chemistry. Raw reads were processed using RDP pipeline version 10 to sort the sequence data by barcodes, to trim  barcode and forward primer sequences, and to filter out low quality sequences (qual below 20) and short reads (length below 250bp).
Wayne State University
Ballast Project 5