GSM1940289: H3K4ME3_FW_03; Mus musculus; ChIP-Seq
Chip-Seq: DNA samples were end repaired, poly-A tailed and Illumina single end adapters were ligated following the standard Illumina protocol with minor adjustments. Agencourt AMPure XP beads at 0.8x ratio were used to size select out adapter dimers after adapter ligation.  The Illumina kit Phusion enzyme was replaced by Kapa HiFi HotStart ready mix. Post PCR, AMPure XP beads were used at a 1:1 ratio to maintain size integrity and to allow size select on 2%Agarose gel.  Samples were finally purified with QAIquick gel extraction kit and quality controlled on the DNA 1000 BioAnalyser 2100 chip. Samples have been multiplexed and run on Hi-seq 2000/1000 (6 samples per multiplexed lane).
GEO
GSM1940289
GEO Accession
GSM1940289