GSM4493873: Sample_14 (WTSH); Mus musculus; RNA-Seq
Hippocampi were extracted and then rapidly homogenized in Trizol. Total RNA was precipitated using isopropanol following the manufacturer's protocol and treated with DNaseI. RNA quality was checked using Nanodrop and a Qubit 4 fluorimeter (Invitrogen). 150bp paired-end mRNA libraries were prepared using the TruSeqv2 (Illumina, #RS-122-2001) LS protocol. Briefly, poly-A mRNA was pulled down using poly-T magnetic beads, fragmented, and primed with random hexamers before first-strand synthesis. Following second-strand synthesis, blunt end repair was performed with a 3' to 5' exonuclease, and 3' ends adenylated. Adaptors were then ligated to the cDNA. All 24 library samples were quality checked on a Agilent 2100 bioanalyser and assayed on a Qubit 4 fluorimeter.
GEO
GSM4493873
GEO Accession
GSM4493873