GSM2570002: MCF7_Mock_ChIP_H3K27ac; Homo sapiens; ChIP-Seq
ChIP DNA was prepared as described previously by our group (O’Hagan et al. 2011).  Briefly, following drug treatment, cells were crosslinked with 37% formaldehyde and nuclei were subsequently extracted.  Nuclear lysate was sonicated on Diagenode Bioruptor until majority of chromatin fragments were in the 100-300 bp range.  Antibodies were incubated with sonicated chromatin overnight at 4°C, followed by capturing primary antibodies by adding ProteinA/G magnetic beads (DynaBeads) and incubating at room temperature for 3 hrs.  Captured antibody complexes were then washed with low and hi-salt buffers.  Chromatin was eluted off of the beads in elution buffer and cross-linking was reversed with an overnight incubation at 65°C.  Chromatin fragments were then incubated with Rnase A for 1 hr at 37°C, and then Proteinase-K for 2 hrs at 55°C. Libraries were constructed according to the protocol provided by Illumina’s TruSeq DNA Sample Preparation v2 Kit
GEO
GSM2570002
GEO Accession
GSM2570002