Amplicon-seq of unpassaged chikungunya live attenuated vaccine candidates
Total RNA was extracted from stock cDNA clone-derived virus and depleted of rRNA using NEB Human/Mouse/Rat rRNA depletion kit. 10^6 genomes as measured by qRT-PCR were used as input for PrimalSeq multiplexed amplicon-based Illumina library generation pipeline using custom primers designed with PrimalScheme.
SUB7609250
CHIKV-181/25-P2.P4.E3/E1-0.1