GSM3929382: F11307: Unspecific immunity line 2; Tribolium castaneum; RNA-Seq
Pooled larvae were immediately shock frozen in liquid nitrogen and stored at -80oC until further use. RNA was extracted using the mirVana miRNA Isolation Kit (Ambion) according to the protocol of the manufacturer. The libraries for Illumina sequencing were prepared using the TruSeq RNA sample V2 Kit (Illumina) following the manufacturer's protocol. The libraries were then clustered on a cBot with the TruSeq PE Cluster Kit V4 (Illumina) with a final loading concentration of 10 pM according to the instructions of the manufacturer.
GEO
GSM3929382
GEO Accession
GSM3929382