Whole Genome Sequencing of Saccharomyces cerevisiae from New Zealand: Sample 8-T.52_3A
Genomic DNA was sheared into smaller fragments of a desired size by a Covaris E210. The overhangs resulting from fragmentation were converted into blunt ends by using T4 DNA polymerase, Klenow Fragment and T4 Polynucleotide Kinase. After adding an "A" base to the 3' end of the blunt phosphorylated DNA fragments, adapters were ligated to the ends of the DNA fragments. The desired fragments were purified though gel-electrophoresis, then selectively enriched and amplified by PCR. The index tag was added to the adapter at the PCR stage.
The University of Auckland
8-T.52_3A