454 deep sequencing of H5N1 influenza: Adaptive mutations - p10 lung
Roche high pure viral RNA extraction kit; Superscript RT III for cDNA sythesis with primers described in PMID 24725402;Amplitaq PCR with primers described in PMID 24725402; Qiagen PCR purification kit; fragment pool in equal concentrations before library prep.
Erasmus MC
p10 lung