Transcriptome sequencing of Hep2(Clone 2B) (HeLa) cells
Hep2 (clone 2B) cells were purchased from Sigma-Aldrich (catalog number: 85011412-1VL) on June 19, 2013 and cultured in Dulbecco's modified Eagle medium (DMEM; Invitrogen) supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (Pen-Strep; Life Technologies) at 37°C in a 5% (vol/vol) CO2 atmosphere. Upon confluence, cells contained in a T75 flask were harvested in a 15ml Falcon tube after trypsinization, washed in Earle's balanced salt solution (EBSS) and centrifuged at 1,100 g for 5 min. Total RNA was then extracted using the AllPrep DNA/RNA Mini Kit (Qiagen). A paired-end stranded RNA library was constructed with the RNA sample after rRNA depletion. The library was tagged and sequenced on an Illumina HiSeq2500 platform in a 2 x 125 bp configuration in High Output mode (V4 chemistry).
OD-phs001944
1_ATTACTCG-TAATCTTA_L005