GSM4064126: Treg_Veh [S3]; Mus musculus; RNA-Seq
RNA was isolated using the RNeasy Micro kit (Qiagen cat#74004). All subsequent sample quality assessment was performed on a Fragment Analyzer electrophoresis system (Agilent). Total RNA was normalized prior to oligo-dT capture and cDNA synthesis with SMART-Seq v4 (Takara cat#634892). The resulting cDNA was quantified using a Qubit 3.0 fluorometer (Life Technologies). Libraries were generated using the Nextera XT DNA Library Prep kit (Illumina cat#FC-131-1096). flow cell using a 100 base pairs, Paired End run. Medium depth sequencing (25 million reads per sample) was performed with a HiSeq 2500 (Illumina) on a Rapid Run.
GEO
GSM4064126
GEO Accession
GSM4064126