Metagenomics of sea water surrounding sponge communities from Algarve, Portugal
Genomic DNA was extracted with PowerSoil DNA Isolation Kit (MoBio, Carlsbad, CA, USA) according to the manufacturer's instructions. Amplicon from V4 region of 16s rRNA gene was amplified using degenerated primers Forward 5’ TACNVRRGTHTCTAATYC 3’ and Reverse 5’ AYTGGGYDTAAAGNG 3’. The primer includes a barcode sequence of eight nucleotides on 5’ end which was unique for each sample.
SUB2375796
Alg.SW.1