Illumina HiSeq 2000 sequencing; Targeted DamID-seq of the Drosophila carpet glia
Targeted DamID-seq of the Drosophila carpet glia
5000 eye imaginal discs were dissected at mid-3rd instar larvae for each sample followed the description in Marshall et al., 2016 doi:10.1038/nprot.2016.084 in brief, dissected eye imaginal discs were mechanically disrupted and genomic DNA was extracted with QIAamp DNA Micro Kit (qiagen# 56304), then digested with DpnI to obtain methylated DNA. methylated DNA after DpnI digestion was ligated with the designed adaptors for further PCR. Ligated DNA was first digested with DpnII and then PCR with designed primers. Designed adapter sequences and primer sequences are as follows:AdRt oligo_5' CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGA3'; AdRb oligo_5'TCCTCGGCCG3'; DamID_PCR oligo_5'GGTCGCGGCCGAGGATC3'
Department of Life Sciences, National Cheng Kung University
E-MTAB-7475:Sample 2_s
Experimental Factor: genotype
C135>UAS-Dam-POLII