GSM4064947: D4lo RNA-seq, replicate 2; Homo sapiens; RNA-Seq
Cells were stained with surface markers and CaspGLOW kit (eBioscience) and sorted at day 4 as D4lo cells and at day 5 as P2/CD23+ (VPDlo/CD38lo/CD23+), P2/CD23- VPDlo/CD38lo/CD23) and P1 (VPDlo/CD38hi) cells using a FACsARIA cell sorter (BD Biosciences). RNA extractions were performed with the NucleoSpin® RNA XS (Macherey-Nagel). QC, library preparation and sequencing were performed by Helixio Company (Clermont-Ferrand, France). After QC by BioAnalyzer, Nanodrop and Qubit, libraries were prepared with the TruSeq Stranded mRNA Library Prep Kit (Illumina).  Librairies were sequenced on an Illumina NextSeq 500 using 75-bp single-end reads (NextSeq 500 High Output v2, Illumina).
GEO
GSM4064947
GEO Accession
GSM4064947