GSM1697492: WT, HisOH; Homo sapiens; RNA-Seq
Total RNA was extracted from cells with RNAzol RT (SIGMA, R4533). For ATF4 KO samples, total RNA was pooled from 3 independent ATF4 KO cell lines. RNA-Seq libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit (New England BioLabs), including poly(A) selection using the NEBNext Poly(A) mRNA Magnetic Isolation module. Final libraries were analyzed with a Qubit fluorometer (Life Technologies) and Bioanalyzer 2100 (Agilent Technologies) to assess quantity and quality. Libraries were clustered at a density of 6.5 pM with a cluster station. Paired-end sequencing was performed using a GAIIx Genome Analyzer (Illumina).
GEO
GSM1697492
GEO Accession
GSM1697492