16S rRNA metabarcoding survey (V3-V4 hypervariable regions) of the microbiome in spontaneous sputum samples from patients with bronchiectasis
V3-V4 hypervariable regions of the 16S rRNA gene were amplified from DNA extracts and sequenced using the 16S metagenomic sequencing library preparation protocol (Illumina, San Diego, CA, USA).
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105st2