Faculty of Animal Science and Technology, Yunnan A : Zhenyuan rumpless chicken
For Illumina pair-end sequencing of each sample, at least 3μg genomic DNA was used for sequencing library construction. Paired-end libraries with insert sizes of ~300bp were prepared following Illumina’s standard genomic DNA library preparation procedure. Purified genomic DNA is sheared into smaller fragments with a desired size by Covaris, and blunt ends are generated by using T4 DNA polymerase. After adding an ‘A’ base to the 3' end of the blunt phosphorylated DNA fragments, adapters are ligated to the ends of the DNA fragments. The desired fragments can be purified through gel-electr ophoresis, then selectively enriched and amplified by PCR. The index tag could be introduced into the adapter at the PCR stage as appropriate and we did a library quality test. At last, the qualified Illumina pair-end library would be used for Illumina Hiseq2500 sequencing (125*2).
Faculty of Animal Science and Technology, Yunnan A
Zhenyuan rumpless chicken