DNA-Seq of female Rattus norvegicus: fecal sample
DNA isolation was performed using physical disruption followed by DNA purification with a series of commercial spin-columns (Zymo Research, D6010), according to manufacturer specification.  Bead beating was performed for 5 minutes in total using 5x 1 minute intervals (MP FastPrep-24 5G).  Total DNA was then profiled by shotgun metagenomic sequencing under commercial contract (Zymo Research).  Sequencing libraries were prepared with the KAPA HyperPlus kit, using 100 ng of input DNA per sample.  Libraries were barcoded using TruSeq LT adapters, checked for quality, and then evenly pooled; sequencing was performed using the Illumina HiSeq platform.
SUB2119632
zr1444_2