GSM1617474: wt_ax_5; Mus musculus; RNA-Seq
Total RNA was extracted from the somatodendritic and the axonal compartment using the Arcturus PicoPure RNA Isolation Kit (Applied Biosystems) with 10 μl elution volume. cDNAs were first amplified using a double-random priming strategy followed by library construction with the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB). Briefly, total RNAs was reverse-transcribed with an oligonucleotide used previously for whole genome amplification [multiple annealing and looping-based amplification cycles (MALBAC)] containing a 3' random octamer and a 5' adapter sequence. The same oligonucleotide was used for one round of second strand synthesis generating cDNA fragments that harbor the adapter sequence in a reverse-complementary manner at both ends. These fragments are then amplified by PCR using adapter oligonucleotides to produce sufficient cDNA amounts for generating high-throughput sequencing libraries.
GEO
GSM1617474
GEO Accession
GSM1617474