Virome Sequencing of Agelas_oroides : Individual_5 mesohyl
Virus like particles were purified via cesium chloride gradient separation. Virion-containing layers (CsCl density 1.2-1.5) were collected and viral DNA/RNA was extracted. Viral nucleotides were randomly amplified using a modified version of the Whole Transcriptome Amplification Kit 2 (WTA2, Sigma Aldrich). This allows the capture of single-stranded and double-stranded DNA and RNA viruses with little amplification bias (as sequence reads represent viral genome copies). NexteraXT libraries were prepared and sequenced on a Hiseq2500 run with 2x250 bp paired end reads
SUB5194334
agelas_m_5