GSM1400870: YYJ_input; Homo sapiens; MeDIP-Seq
DNA fragment were sonicated, and isolated by anti-5mC antibody Genomic DNA was fragmented to 100–500 bp by sonication. Adaptors were ligated to both ends of the fragments. End repair, base addition, and adaptor ligation steps were performed by using Illumina’s Paired-End DNA Sample Prep kit following the manufacturer’s instructions. Adaptor-ligated DNA was immunoprecipitated with anti-5mC antibody, and the MeDIP products were validated by qPCR using SYBR green mastermix. To obtain genomic DNA libraries that were suitable for the Illumina Genome Analyser II, the DNA was amplified, purified, and size selected (200–300 bp, including the adaptor sequence). The sequences of each individual were generated as 44-bp paired-end reads.
GEO
GSM1400870
GEO Accession
GSM1400870