Illumina HiSeq 1500 paired end sequencing of SAMD00089606
To compare the amplification accuracy between TruSeq library prep kit (Illumina) and SMART-Seq v4 kit (Clontech), RNA-seq libraries were constructed from the same RNA. The spores of R. irregularis DAOM181602 were incubated in liquid M medium for 1 day at 28????C. Total RNA was extracted by using RNeasy Plant Mini kit (Qiagen). RNA quality and quantity were checked by using Agilent 2100 Bioanalyzer and Qubit (Thermo Fisher Scientific). Libraries were constructed from 100 ng of RNA by using TruSeq library prep kit. After the quality and quantity were confirmed by using Agilent 2100 Bioanalyzer and KAPA Library quantification kit, the libraries were sequenced by HiSeq 1500.
NIBB
DRX093134