GSM3928801: sRNA control shoot; Oryza sativa; miRNA-Seq
Total RNA was extracted from the individual samples using TRIzol reagent (Thermo Fischer Scientific) following manufacturer's instructions. Only good quality RNA with RIN value above 7.0 was used for downstream processing. Total RNA (1 µg) from each sample was subjected to electrophoresis on denaturing polyacrylamide gel to separate out the sRNA fragments Small RNAs recovered were ligated with 3'- and 5'- adaptors. The ligated sRNA products from each sample were reverse transcribed and PCR amplified to generate the sRNA libraries. The individual libraries were normalized to 2 nM with Tris-HCl as per TruSeq Small RNA Prep kit manual (Illumina). The adapter libraries prepared were processed for single-end sequencing on HiSeq2000. The sequencing results were generated as FASTQ data using Illumina pipeline CASAVA 1.8 package. Therefore, the current study was planned to see the expression profile of miRNAs in a salt-tolerant rice (Oryza sativa L. ssp indica) cultivar (cv.) Pokkali in presence and absence of NaCl and to check the response pattern of a few important salt-responsive miRNAs in a salt-sensitive rice cultivar Badami in order to identify the miRNAs and their targets that could be involved in the salt tolerance process
GEO
GSM3928801
GEO Accession
GSM3928801