GSM4492130: P-5874; Homo sapiens; RNA-Seq
Fresh GCT tumours collected after surgery were dissociated in Collagenase-Dispase (Sigma-Aldrich). Red blood cells were lysed by ammonium chloride treatment for 5 min on ice. Cell viability was assessed with Trypan Blue. For samples with low viability (<60%), dissociated cells were enriched for live cells using the Dead Cell Removal kit (Miltenyi Biotech). 10,000 dissociated cells per sample were loaded on the 10X Genomics Chromium controller. The Chromium Single Cell 3′ (10X Genomics, Version 3) protocol was strictly followed to prepare libraries. The 10X libraries were sequenced on the Illumina HiSeq 4000 sequencing platform.
GEO
GSM4492130
GEO Accession
GSM4492130