Bacterial diversity of unexplored co-occurring marine samples of Palk Bay by Illumina sequencing
Amplicons were constructed with the starting amount of 20ng/µl and 20 cycles of PCR was performed to amplify the region of interest using specific primers. The amplified region was gel eluted using Qiagen minelute columns. Adapter and index sequences were incorporated in the second round of PCR. 6 cycles of PCR was performed and the product was cleaned up using Agencourt Ampure XP SPRI beads (Beckman Coulter). The prepared library was quantified using Nanodrop and validated for quality by running an aliquot on High Sensitivity Bioanalyzer Chip (Agilent). PCR's were done with 2X KAPA PCR Hotstart Ready mix for amplification.
Alagappa University
SW