Raw genomic sequence data for a deletion mutant from Mycoplasma mycoides subsp. capri strain GM12, YCpMmyc1.1-deltaglf
High molecular weight DNA was sheared in a Covaris g-TUBE (Covaris, Woburn, MA, USA). DNA size distribution was checked on a Fragment Analyzer (Advanced Analytical Technologies, Ames, IA, USA). 6 µg of the sheared DNA was used to prepare a SMRTbell library with the PacBio SMRTbell Template Prep Kit 1 (Pacific Biosciences, Menlo Park, CA, USA) according to the manufacturer's recommendations. 1.6 µg of the resulting library was size selected on a BluePippin system (Sage Science, Inc. Beverly, MA, USA) for molecules larger than 10 kb.
International Livestock Research Institute
YCpMmyc1.1-deltaglf