GSM4618361: ChIC-seq CBFB-SMMHC knock-in Runx1 knock-out Runx1 rep1; Mus musculus; OTHER
AMP cells were fixed with 1% PFA and subjected to ChIC-seq as described.7 Specifically, antibody+PA-MNase complex with 50,000 cells was used for each sample. RUNX1 (Abcam, AB_2184205), CBFβ (Diagenode, C15310002), MYH11 (Diagenode, C15310254), H3K27Ac (Abcam, AB_2118291) and H3K27me3 (Millipore Sigma, AB_310624) antibodies were used to identify the binding sites of the indicated proteins. All libraries were combined in equimolar proportion into one pool for sequencing with a HiSeq 3000 sequencer
GEO
GSM4618361
GEO Accession
GSM4618361