Control Diet + Vehicle - 4 metatranscriptomics
Total RNA was first treated with rDNase I. Bacterial rRNAs were depleted using the MICROBExpress kit and host rRNAs were depleted with the NEBNext rRNA Depletion Kit for Human/Mouse/Rat. Metatranscriptomic libraries were prepared from the depleted RNA using the NEBNext Ultra II Directional RNA Sequencing Prep Kit to produce libraries that averaged between 200-450 bp. Libraries were then sequenced on an Illumina HiSeq X Ten
SUB6256653
JW28