GSM4221645: 10 µM DD, 3 h, rep 2; Phaeodactylum tricornutum; RNA-Seq
Cultures were harvested by vacuum filtration (Whatman GF/FNuclepore 0.4 µM filters). Cells on filters were frozen by liquid nitrogen and stored in - 80˚C. Cells were resuspended in PureLinkTM Plant RNA Reagent (12322012 Thermo Fisher) according to the manufacturer's protocol. Samples were mechanically disrupted in QIAGEN Tissue LyserLT (2 X 2 min, 1 X 1 min, 30 sec pauses, 50 oscillations/sec) with Zirconia-silica beads (0.5 mm, Biospec). Total RNA was purified according to the PureLinkTM Plant RNA Reagent protocol. Poly (A) enrichment using Poly (A) Purist Magnetic Kit (Thermo Fisher) and stranded RNA-Seq library was prepared using NEBNext reagents (New England Biolabs)
GEO
GSM4221645
GEO Accession
GSM4221645