RNA-Seq of enriched human leukemia or lymphoma cells from spleens of patient-derived xenograft models.
mRNA was extracted from PDX leukemia or lymphoma cells using magnetic microbeads (mMACS mRNA Isolation Kit; Mitenyi Biotec). Non-stranded RNA-seq libraries were generated using the True-SeqRNA Sample Prep kit (Illumina) on a Sciclone platform (Perkin Elmer). mRNA underwent fragmentation, cDNA synthesis, and next-generation library synthesis via end repair, adenylation, adapter ligation, and PCR amplification. Libraries were sequenced on a Next-Seq instrument (Illumina) using a paired-end protocol.
SUB1899655
BCAT-83146-V2