GSM2481124: Normoxic; Chlamydia pneumoniae; RNA-Seq
total RNA of normoxic and hypoxic chlamydial infected HEp-2 cells was isolated by NucleoSpin RNA kit (Macherey Nagel). Human rRNA was depleted by RiboZero rRNA removal kit (Epicentre) in order to enrich bacterial RNA. The tagged cDNA libraries, in the size range of 250 –400 bp, were pooled and single-read sequencing (read length 50 bp) was performed on Illumina HiSeq 2000 by BGI-Hong Kong RNA libraries were prepared for sequencing using standard Illumina protocols
GEO
GSM2481124
GEO Accession
GSM2481124