RNA-Seq of Melampsora lini  germinated urediniospores - replicate 1
Total RNA was isolated from up  to 200 mg Linum usitatissium leaves inoculated by Melampsora lini at 8 days  post inoculation using the RNeasy midi kit (Qiagen), according to the  manufacturers instructions. Libraries  were made and 50 bp single reads were generated in one lane with samples of  Linum usitatissium leaves inoculated by Melampsora lini at 6 days post  inoculation according to standard Illumina HiSeq protocols. The sequence reads were analysed according  to Australian Genomic Research Facility quality control measures. Sequence quality was >95% bases above  Q30. The reads were also screened for  the presence of any Illumina adaptor/overrepresented sequences and  cross-species contamination. The  primary sequence data was generated using the Illumina consensus assessment  of sequence and variation (CASAVA) pipeline (version 1.8.2).
SUB5910978
MlgsA