Amauroascus mutatus cDNA paired-end reads
For total RNA isolation, isolates were grown on cellophane under a variety of conditions for 14 days and snap frozen in liquid nitrogen: (i) Sabouraud glucose agar (Greer 1999) at room temperature, (ii) Sabouraud glucose agar (Greer 1999) at room temperature with a 1 hour incubation at 50oC prior to sample collection, (iii) Hay-infusion agar (CBS-KNAW 2012) at room temperature, and (iv) Oatmeal-salts agar (Greer 1999) at room temperature. Total RNA was extracted and purified using a previously described protocol (Kasuga et al. 2005). Illumina paired-end libraries were prepared using the standard TruSeq PE RNA Sample Prep Kit (Illumina Inc., San Diego CA, USA) protocol using a starting amount of 2-4µg total RNA. Four conditions per isolate (see above) were prepared, indexed and combined into one sequencing lane per isolate; Illumina indexes 4 (i samples), 15 (ii samples), 6 (iii samples), and 7 (iv samples).
UC Berkeley
Amauroascus mutatus cDNA