Soil fungi from Bolivian Altiplano fallow fields
The fungal Internal Transcribed Spacer region (ITS) was PCR-amplified using the forward primer (ITS1-F) and  the reverse primer (ITS4). These primer constructs combined 454-sequencing primer (A-primer) and the reverse primer (ITS4) with a five base pair (bp) DNA tag for post-sequencing sample identification in between, or the DNA capture bead anneal primer (B-primer) for the emulsion PCR (emPCR) and the forward primer (ITS1-F).
Kansas State University
Bolivia ITS