Sample_19 BB_Low Strongylocentrotus droebachiensis mRNAseq
Illumina TruSeq v1 kit- Larvae exposed to control or acidified         seawater. Larvae were grown in either control seawater or seawater acidified with bubbled CO2 gas to lower the pH by 0.4 units. After 5-9 days, approximately 3000 larvae were collected, lysed, and preserved in RLT Buffer (Qiagen). Total RNA was extracted with the Qiagen RNeasy kit. Libraries were prepared using the Illumina TruSeq v1 kit with poly-A selection by the Duke IGSP Genome Sequencing & Analysis Core and sequenced 6 samples / lane in 7 lanes.
Duke University
Sample_19