GSM3538767: 04T144; Homo sapiens; miRNA-Seq
Tissues were fresh frozen in OCT. RNA was harvested using Trizol reagent. 2µg of total RNA samples were used and  were processed with kit "NEXTflex Illumina Small RNA Sequencing Kit v3" (Bioo Scientific catalog# 5132) by following manufacturer instructions. Briefly, samples were sequentially ligated to randomized 3' and 5' adapters, with a truncated form of T4 RNA Ligase 2 and the T4 RNA ligase 1, respectively. Reverse transcription with a MMuLV Reverse Transcriptase yielded cDNA adapter-ligated libraries that were amplified by 14 cycles of PCR. Amplified products were either purified by double-sided SPRI selection of by PAGE extraction, according to their BioAnalyzer size profile. Pooled purified fractions were applied to an Illumina flow cell for cluster generation, and sequenced on an Illumina HiSeq2000 by following manufacturer's protocols. Sequencing read ends contain a variable length of the 3' adapter, whose sequence is nnnnTGGAATTCTCGGGTGCCAAGG. As a consequence of the use of adapters with random tetramers, both the first four bases of the read and the four bases at the beginning of the 3' adapter are noninformative. Following 3' adapter trimming, those first and last four bases of the read were trimmed too.
GEO
GSM3538767
GEO Accession
GSM3538767