GSM2666463: PDlC-D3 mRNA; Homo sapiens; RNA-Seq
mRNA: Total RNA was extracted from cell or animal tissue by Trizol reagent (Invitrogen) separately. The RNA quality was checked by Bioanalyzer 2200 (Agilent) and kept at -80℃. The RNA with RIN >8.0 is right for cDNA library construction. miRNA: Total RNA was extracted from cell or animal tissue by Trizol reagent (Invitrogen) separately. The RNA quality was checked by Bioanalyzer 2200 (Agilent) and kept at -80℃. The RNA with RIN >8.0 is right for miRNA purification. miRNA was purified by miRNeasy Mini Kit (Qiagen) and the purification result was validated by gel electrophoresis. RNA-Seq: RNA libraries were prepared for sequencing using standard Illumina protocols. miRNA-Seq: RNA libraries were prepared for sequencing using standard Ion Proton protocols.
GEO
GSM2666463
GEO Accession
GSM2666463