GSM3928686: PLC-shUSP22-1; Homo sapiens; RNA-Seq
Samples were immediately frozen in liquid nitrogen and stored at  -80℃ until further use.RNA was harvested using Trizol reagent.  NEBNext® Ultra™ RNA Library Prep Kit for Illumina (NEB, USA) was used with 1 ug of total RNA for the construction of sequencing libraries. mRNAs were isolated from Total RNA and fragmented to approximately 200bp.Subsequently, the purified  RNAs were subjected to first strand and second strand cDNA synthesis following by adaptor ligation and enrichment with a low-cycle according to instructions of NEBNext® Ultra™ RNA Library Prep Kit for Illumina (NEB, USA).
GEO
GSM3928686
GEO Accession
GSM3928686