RNA-seq of mus musculus: adult female alveolar macrophages
Alveolar macrophages were enriched from total BAL cells using adherence on tissue culture dishes. AMs from 2-3 mice were pooled for each sequenced sample, and total RNA was isolated using the Qiagen RNeasy Plus Micro Kit. Library preparation and rRNA depletion were performed using the Takara SMARTr Ultra low-input RNA-seq library prep kit.
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