miRNA-Seq of Linum usitatissimum: symplastically growing cortical parenchyma
Symplastically growing cortical parenchyma (cPAR) isolated by laser microdissection (1st cm and 3rd cm from apex) Total RNA from the cPAR sample was isolated using Trizol combined with a mirVana miRNA Isolation Kit (Thermo Fisher Scientific, Lithuania) and library of small RNA were prepared with a NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs, UK) Quality control of the library was performed using an Agilent 2100 Bioanalyzer (Agilent Technologies, USA). Further size selection was performed using Agencourt AMPure XP (Beckman Coulter, USA) to select for 143149 bp fragments. Raw reads from run were filtered to remove adapter sequences, contamination, low-quality reads and any reads shorter than 15nt and longer than 30nt.
SUB5185332
Lus_small_cPAR_2