Tn-Seq sample from Bacillus subtilis 168 harvested at onset of starvation (T0)
Tn-seq sample of Bacillus subtilis at the onset of starvation (T0). A magellan6 transposon library was grown in Difco Sporulation Medium to the onset of starvation (T0). A sample was harvested and genomic DNA extracted.  The library was constructed by MmeI digestion of genomic DNA and adapter ligation, followed by a 16 cycle PCR amplification to select for transposon insertion sites. This sample was sequenced on the Illumina HiSeq 2500 platform.
Harvard University
T0