Mesembryanthemum crystallinum genomic DNA
The common ice plant (Mesembryanthemum crystallinum_L., Azoaceae, Caryophyllales) is an important halophytic and facultative crassulacean acid metabolism (CAM) model in which CAM can be induced by salinity or water-deficit stress. To improve our understanding of the molecular genetic basis of CAM, water-use efficiency, and salinity tolerance in plants, a high-quality, annotated genome assembly was generated for ice plant using two different genome sequencing platform, Illumina and PacBio, resulting in a depth of coverage of 254X and 90X, respectively. We assembled the PacBio reads with SMRTanalysis. Illumina mate-pair reads were used for scaffolding with the resulting assemblies. To improve the accuracy and completeness of the genome assembly, Illumina paired-end reads were used to polish the resulting scaffold. The polished assembly was then used for BioNano Genomics Irys_ single-molecule next-generation mapping (NGM) to improve the quality of the genome assembly. This high-quality draft genome assembly will enable the identification of the vast majority of genes required for the operation of CAM and for the adaptation of this halophytic species to salinity and drought stress conditions, comparative CAM genomics efforts, and CAM biodesign projects aimed at introducing the improved WUE of CAM into C3 or C4 photosynthesis crop plants.
SUB2119952
Illumina_mate_pair_10kbp_gDNA_22