GSM4174604: HEC-1-B_WT_rep2; Homo sapiens; RNA-Seq
Total RNA from culture cells was extracted using Trizol reagents (Life Technologies) following the manufacturer's instructions. Total mRNA was prepared from 1 mg total RNA using poly(A) mRNA magnetic isolation reagents (NEB) and fragmented at 94 °C for 15 min. RNA was then reverse-transcribed into cDNA with random primers. After end repairing and A-tailing, cDNA was ligated with adapters and amplified by PCR with Illumina sequencing primers. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO
GSM4174604
GEO Accession
GSM4174604