GSM3929395: Midbrain organoids LRRK2-WT differentiated for 35 days; Homo sapiens; RNA-Seq
Single cell RNA seq using Drop-seq - 3' bead based enrichment technique, using SMART synthesis (Macosko et al., 2015 doi: 10.1016/j.cell.2015.05.002). Libraries were prepared according to Drop-seq methods (Macosko et al., 2015 doi: 10.1016/j.cell.2015.05.002). After cDNA tagmentation, PCR amplification was performed using Illumina primers (N701, N702, A501). The purified cDNA fragments of ~600 bp were captured on an Illumina flow cell for cluster generation. Libraries were sequenced on Illumina NextSeq 500 following the manufacturer's protocols.
GEO
GSM3929395