medaka HNI blastula RNA-seq
RNA was isolated from medaka HNI blastula using ISOGEN (Nippon Gene) and RNeasy mini kit(QIAGEN). Purified RNA were treated with Ribominus eukaryote kit for RNA-seq (Life Technologies). RNA-seq library was prepared using TruSeq RNA-seq sample prep kit (Illumina). The PCR products were purified and size fractionated using a bead-mediated method (AMPure, Ambion). Sequencing was conducted on HiSeq 2500 platform (Illumina).
the University of Tokyo
medaka HNI blastula RNA-seq