Eucalyptus transcriptome under  nutrient stress
RNA was extracted using  PureLink Plant RNA Reagent (Invitrogen) as manufacturer instructions. To  remove any trace of contaminating genomic DNA, RNA elutes were subjected to a  DNase treatment. RNA quantity was measured by spectrophotometer (Thermo  Scientific NanoDrop 1000), and its integrity was verified by capillary  electrophoresis (Agilent Bioanalyzer 2100) using RNA 6000 Nano kit (Agilent  Technologies). Only high quality mRNA were converted into cDNA libraries  using Illumina kit (Illumina, Inc.) for a single lane run in HiSeq 2500  (single-end 51 bp).
SUB5912840
L3P2