PacBio ISO-Seq Denovo of Cinnamomum parthenoxylon
The study constructed 2 ISO-Seq libraries for 1 sample and sequenced 2 cells in total with PacBio Sequel platform.Before sequencing, the library should be built according to the length of different inserted fragments. In this way, the long and low abundance transcriptome segments can be sequenced sufficiently. The strategy of fragment selection is to build a mixed library with a fragment free Library and a >4K fragment length Library,and then the hybrid library was sequenced.
SUB6566766
HZ