Brasicca napus 14-20 DAF
Total RNA from frozen tissue of B. napus was extracted using TRIzol Reagent and mRNA was purified using oligo dT purification kit. cDNA synthesis was with Creator SMART cDNA Library Construction Kit (Clontech), using LD PCR. First strand cDNA was synthesized from 2.0 *g mRNA using SUPERSCRIPT II reverse transcriptase (Invitrogen), and CDS III /3’ primer (5’tagaggccgaggcggccgacatgttttgtttttttttcttttttttttvn3’).  Sequencing with 454 Titanium.
MSU-RTSF
b_napus_14_20_exp