GSM3005273: liver_zonation_5_KO_no_infusion; Mus musculus; RNA-Seq
Liver were removed, flash frozen liquid nitrogen, and RNA was harvested using Trizol reagent. Total RNA was purified from all samples using MagMAX™-96 for Microarrays Total RNA Isolation Kit (Ambion by Life Technologies) according to manufacturer's specifications. Genomic DNA was removed using MagMAX™Turbo™DNase Buffer and TURBO DNase from the MagMAX kit listed above (Ambion by Life Technologies). mRNA was purified from total RNA using Dynabeads mRNA Purification Kit (Invitrogen). Strand-specific RNA-seq libraries were prepared using KAPA mRNA-Seq Library Preparation Kit (Kapa Biosystems).
GEO
GSM3005273
GEO Accession
GSM3005273