Bacterial 16S rRNA gene amplicon sequencing of Spartina anglica rhizosphere: control
Genomic DNA from soil samples was extracted using a MoBio PowerSoil DNA extraction kit. Amplicons were prepared from total DNA by PCR targeting the bacterial 16S rRNA gene (forward: 515F: GTGCCAGCMGCCGCGGTAA and reverse: 806R: GGACTACHVGGGTWTCTAAT) (Caporaso et al., 2012 ).  PCR amplicons were quantified using PicoGreen, normalized at 1 ng l-1, pooled together and sent for sequencing on an Illumina MiSeq (paired-end 2x250bp) at the McGill University and Genome Quebec Innovation Center (Montral, Canada).
SUB5194387
Bacteria.21.San.C3