Paired End Illumina MiSeq of PF T. brucei brucei mitochondrial mRNA; RPS12 in MRB7260 RNAi uninduced
Cells were grown for three days either induced (plus_TET) or uninduced (minus_TET) and whole RNA was extracted.  CDNA was generated using the 3' primer specific to each transcript and libraries were generated for each transcript, maintaining amplification within the linear range of the PCR machine.  Whole populations of pre, fully and partially edited mRNAs were sequenced using paired-end Illumina MiSeq.
SUB2752470
PhyH-KD-RPS12-2-minus