Chinampa soil 16S rRNA bacteria and archaea amplicons obtained by 454 technology
Metagenomic DNA was obtained from soil collected from chinampa soil, in Mexico City. The V1-V3 hyper variable regions of the bacteria 16S rRNA gene and V3-V5 of Archaea 16S rRNA were amplified using primers containing a MID sequence of 10 nt, 454 pyrosequencing adaptators Lib-L, and universal custom primers. We obtained 6 libraries of Domain Bacteria and 2 of Domain Archaea.
Escuela Nacional de Ciencias Biológicas
16S rRNA Bacteria and Archaea