ChIP-Seq analysis of H3K27me3 in human hN (ES H9 derived Neurons) cells
Single read - Illumina
BI
Lib:RI:20101012:16--ChIP:MA:20100927:16:hN (ES H9 derived Neurons):H3K27Me3
CHIP_PROTOCOL_BEAD_TYPE
Protein A-Sepharose 4B
CHIP_ANTIBODY
H3K27Me3
CHIP_PROTOCOL_CHROMATIN_AMOUNT
10 million cells
EXTRACTION_PROTOCOL
SDS lysis
CHIP_PROTOCOL_ANTIBODY_AMOUNT
2-15 ug
CHIP_PROTOCOL_BEAD_AMOUNT
70 ul bed volume
CHIP_PROTOCOL
Bernstein_BROAD_ENCODE_protocol
EXTRACTION_PROTOCOL_SONICATION_CYCLES
1
CHIP_ANTIBODY_CATALOG
CS200603
CHIP_ANTIBODY_PROVIDER
Millipore
CHIP_ANTIBODY_LOT
DAM1499359
EXPERIMENT_TYPE
Histone H3K27me3
EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR
Bioruptor
GEO Accession
GSM772787