F757662
Stool collected in 100% ethanol (Lloyd-Price et al., 2019), DNA extracted with combination of QIAamp 96 PowerFecal Qiacube HT Kit, the Allprep DNA/RNA 96 Kit, and IRS solutio kit used with a custom protocol as previously described (Lavoie et al., 2019). Purified DNA was stored at -20 degree celsius. Quantification via Quanti-iT PicoGreen dsDNA Assy, normalized to 50 pg/uL. Libraries preped using Nextera XT DNA Library Preparation kit. Libraries were pooled using equal volumes and sequenced on HiSeq 2x101.
SUB7604342
F757662