Illumina HiSeq 2500 sequencing; RNA Seq of the ventricular region from young and aged killifish
RNA Seq of the ventricular region from young and aged killifish
Cardiac venticles were dissected from young (6 weeks) and aged (12 weeks) killifish on cold PBS. Tissues samples were immediately transfered to cold TRIzol for downstream processing.   Tissues were harvested immediately in cold TRIzol for the RNA isolation. RNA isolation was performed using the standard TRIzol based protocol. Quantity and the purity of the isolated RNA was accessed by NanoDrop™.  Poly-A enriched RNA-seq libraries (2 x 75nt; stranded) were prepared from ventricles of three 6 weeks and three 12 week old animals.
Post Doctoral Fellow
E-MTAB-7582:K002000083_53157_2.fq.gz_s
Experimental Factor: developmental stage
young