Sequencing project for R. flavipes brain tissues
The concentrated RNA was subjected to two rounds of RiboMinus Eukaryote kit for RNA-seq (Invitrogen, Grand Island, NY, USA) to minimize ribosomal RNA before cDNA library construction. Reverse transcription was performed using the cDNA Synthesis System Kit (Roche Applied Science, Indianapolis, IN, USA) following the manufacturer’s protocol. Library construction was performed including MID tagging using the GS FLX Titanium Rapid Library Preparation Kit from Roche according to the manufacturer’s manual. Emulsion PCR was done with GS FLX Titanium SV emPCR Lib-L Kit (Roche Applied Science, Indianapolis, IN, USA) following the manufacturer’s instruction.
University of Kentucky
R. flavipes brain transcriptome