GSM2905811: dCas9-BFP-DNMT3A (500 ng) + uPA gRNA (500 ng) -3; Homo sapiens; Bisulfite-Seq
Genomic DNA was extracted using the DNeasy Blood & Tissue Kit (Qiagen, 69506) according to the manufacturer's instructions, and subject to WGBS SOP analysis in BGI tech. For Bisulfite-Seq, genomic DNA was fragmented by sonication to a mean size of 250bp using a Bioruptor (Diagenode, Belgium). Bisulphite conversion was conducted with the EZ DNA Methylation-Gold kit (ZYMO). The libraries were sequenced on Illumina HiSeq X Ten. The 48502 bp lambda DNA was used as an extra reference for calculating the bisulphite conversion rate. Nearly complete (>99%) bisulfite conversion was documented in all libraries.
GEO
GSM2905811
GEO Accession
GSM2905811