RAD-seq of Bombus lapidarius
Restriction-site associated (RAD) protocol. High quality DNA extraction using the DTAB protocol. Digestion of all DNA samples with restriction enzyme PstI.Ligation of barcoded forward amplification primer. Pooling of barcoded samples and shearing of RAD sample pools to fragment size of about 300-500.Ligation of reverse amplification primer (adaptor set with bar-codes).Selectively amplification of fragments that harbour correctly both adaptors.Sequencing of amplified samples on HiSeq 2500 with chemistry v4.
SUB2375318
LGERDU2