Aluca39_5
We used three adult animals (two males and one female) and 22 larvae (from the cross of two of those three adults) for this study. The adult animals were collected from Bimini, Bahamas and maintained in the laboratory. A moonlight regime (Fishbowl Innovations, Spokane, WA) was used to induce the spawning. The DNA of this pair with 22 of their offspring was used to make the pooled library (approximately 300 bp insert size and individually barcoded). The genomic DNA of all samples was extracted by the DNAEasy kit (Qiagen Inc., Valencia CA, USA) and sequencing libraries were prepared by the Nextera kit (Illumina Inc, San Diego, CA, USA), according to the manufacturers’ protocols. Illumina paired-end sequencing was performed on the Illumina HiSeq2000 platform at BGI (Shenzhen, Guangdong, China).
Rice University
Aluca39_5