MethylC-seq of Brachypodum: BdTR13c_rep2_spring
gDNA isolated from Invisorb plate-based plant kits was processed through Zymo EZ DNA methylation gold 96 Mag-Prep bisulfite conversion under recommended conditions. Libraries created using Swift AccelNGS MethylC-seq kit modified for third reaction volumes and no Covaris fragmentation step. Each sample was given one of 96 possible dual-index barcode combinations and pooled (max 96 sampels) in a HiSeq 2500 run (Single-End 100bp).
SUB2124954
S60029