RNA Sequencing of Vitis vinifera cv. Corinto Bianco, sample CB-2 (project: Analysis of the origin of parthenocarpy in Corinto Bianco grapes)
The library was constructed from flowers of a replicate of Vitis vinifera cv. Corinto Bianco. The library was prepared using the Illumina TruSeq Stranded mRNA Sample Prep Kit starting from 1 ug of total RNA. The adapter-ligated library was amplified with 15 PCR cycles, followed by purification with Ampure beads. Finally, the library was run on a Bioanalyser DNA1000 chip, and quantified using a real-time PCR kit (Kapa Biosciences). Sequencing was performed on the Illumina HiSeq 2000 using v3 chemistry (flowcells and sequencing reagents).
Instituto de Ciencias de la Vid y del Vino
RNA Sequencing of Corinto Bianco, sample CB-2