small RNAs from EBV-positive Jijoye cell line
"RNA was isolated using Trizol according to manufacturer's instructions (Life Technologies). Integrity of RNA was verified by Agilent 2100 bioanalyzer (RIN score was 10.0). Sequencing of small RNAs was performed by BGI (Hong Kong, China). A sequencing library of small RNA was generated as follows: Illumina adapter sequences were ligated to the 3' and 5' ends of size-selected RNA molecules (~18-30 nt length ). Reverse transcription was performed using Superscript II (Invitrogen), followed by PCR amplification and gel purification. Clusters were generated using a cBot amplification system (with TruSeq SE Cluster Kit v3-cBot-HS reagents). Single-end sequencing by synthesis was performed on the HiSeq 2000 system using the TruSeq SBS Kit-HS V3"
University Medical Center Utrecht
small RNA_Jijoye