Nanopore reads for WGS of Cutibacterium acnes strain SZ1
Colonies were incubated with GAM liquid medium; and cultured for 2 days at 37C under anaerobic conditions prior to the isolation of genomic DNA using Genomic-tip 20/G (Qiagen), following the manufactures protocol. Long read sequencing libraries were prepared using the rapid barcoding sequencing protocol (SQK-RBK004) and sequenced on a GridION device with a FLO-MIN 106 flow cell (Oxford Nanopore Technologies).
SUB7020151
nanopore