S12_16S_14
Sequencing at Molecular and Cellular Imaging Center (MCIC) at Ohio State University following the protocols of Caporaso et al. (2012). PCR amplification of the V4 -V5 region of 16S rRNA made use of the forward primer 515F (5- GTGCCAGCMGCCGCGGTAA-3) and the reverse 806R (5-GGACTACHVGGGTWTCTAAT-3). PCR amplicons were quantified by PicoGreen (Invitrogen, Carlsbad, CA) using a plate reader, and amplicons were pooled in equal concentrations into a single tube. Five replicate PCRs were pooled and purified using Agencourt AMPure XP magnetic beads (Agencourt Bioscience Corporation, MA, USA). The pooled samples were sequenced on the Illumina MiSeq platform (Illumina, San Diego, CA) according to the sequencing procedures described in Caporaso et al. (Caporaso et al., 2012).
SUB7608150
S12.16S