BLESS HeLa cell samples treated with Neocarzinostatin (NCS sample)
For BLESS Illumina library preparation, we used the TruSeq DNA sample preparation kit v2 (Illumina) without DNA fragmentation and library size selection. For gDNA sequencing, we sheared gDNA with Covaris S220 AFA (Covaris) according to the manufacturer’s instructions prior to Illumina library preparation. We assessed library quality and quantity on the 2100 Bioanalyzer (Agilent) using the High Sensitivity DNA Kit (Agilent), and by qPCR with the Kapa Library quantification Kit (KapaBiosystems). We generated clusters on the Illumina flow cell using the automatic cBot station and the TruSeq PE Cluster Kit v3-cBot-HS. We carried out sequencing by synthesis on Illumina HiSeq 2000 system using the TruSeq SBS Kit v3-HS chemistry.
UTMB at Galveston
Samples_N1-2