GSM3009763: IFNAR_A_Macr; Mus musculus; RNA-Seq
RNA was prepared according to manufacture's instructions with 1 ml of Trizol being used per 5 million of cells or 50 µg of tissue Library was prepared using Truseq pair-end Illumina kit. Briefly, 10-100 ng of total RNA was reconstituted in water, mixed with Ribosomal removal Buffer containing RNAClean XP beads, and depleted from rRNA. First strand was synthesized using RT, followed by a second strand synthesis, 3' ends were adenylated and ligated to the adaptors, followed by ligateion of the 5' adaptors; the library was purified and measured on DNA-concentration
GEO
GSM3009763
GEO Accession
GSM3009763