GSM1698438: P3_F5; Homo sapiens; RNA-Seq
Total RNA, including the small RNA fraction, was obtained by organic extraction followed by miRNeasy purification (Qiagen). The 3'-DGE libraries were prepared from 20ng of total RNA according to the Single Cell RNA Barcoding and Sequencing method originally developed for single cell RNA-seq (SCRB-seq (Soumillon et al., 2014) and adapted to extracted total RNA. Briefly, Poly(A)+ mRNA from extracted total RNA are converted to cDNA decorated with universal adapters, sample-specific barcodes and unique molecular identifiers (UMIs) using a template-switching reverse transcriptase. Decorated cDNA from multiple samples are then pooled, amplified and prepared for multiplexed sequencing using a modified transposon-based fragmentation approach that enriches for 3' ends and preserves strand information.
GEO
GSM1698438
GEO Accession
GSM1698438