RNAseq of Atlanta ariejansseni: juveniles whole body
RNA was extracted from samples of 810 individuals randomly pooled from each treatment using the RNeasy Plus Micro Kit (QIAGEN) and checked for quality and quantity. Libraries were generated with the NEBNext Ultra II Directional Library Prep Kit for Illumina (New England BioLabs) using the manufacturers protocol for Poly(A) mRNA magnetic isolation from 1 g total RNA. Libraries were checked for quantity and quality on Bioanalyzer 2100 using an Agilent DNA High Sensitivity Chip. Average library sizes of 420 up to 450 bps (~300 bp insert +128 bp sequencing adapters) were accessed using the Agilent TapeStation. All libraries were sequenced producing circa 6 giga base pairs (Gbp) per library.
SUB6536457
A03 replicate 1