Axial post-eruptive plume 16S metagenome
The v4-v5 hypervariable regions of the bacterial and archaeal SSU rRNA gene were amplified using revised degenerate primers 515F and 806R. PCR products were checked in 2% gel agarose, then pooled in equal proportions, then purified and calibrated using AM Pure XP beads. PCR products were sequenced on Illumina MiSeq following manufacturer's guidelines.
SUB3268694
T1501.9