RNA-Seq of zebrafish larvae: wild-type
The total RNA was extracted from 5 dpf larvae using TRI Reagent (Invitrogen, Cat. No. AM9738). Thirty larvae were pulled together to obtain one RNA sample. The RNA quality was verified by measuring the absorbance at 260, 280, and 230 nm. RNA samples were digested with DNase I, and purified with the RNA Clean and Concentrator Kit (ZYMO Research, Cat. No. R1013). The sequencing procedure was performed using Illumina methodology. The preparation of the cDNA libraries and sequencing by Next-Generation Sequencing (NGS NextSeq 500) were performed in cooperation with the Core Facility at the International Institute of Molecular and Cell Biology. The sequencing resulted in approx. 4070 million reads per sample with a 76 bp length. The reads were extracted in FASTQ format and used for the subsequent analysis. The reads were then aligned to the zebrafish RefSeq genome assembly annotated genes using the Ensembl annotation.
SUB7313630
wt-51051