GSM3381519: SD8-7; Homo sapiens; RNA-Seq
Total RNA was extracted from cells using TRIzol reagent (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions. Libraries were prepared according to illumina's instruction accompanying the Truseq stranded mRNA prep kit. Briefly, The polyA containing mRNA molecules were purified using poly-T oligo attached magnetic beads and then fragmented into small pieces. The cleaved RNA fragments were primed into random hexamers into first strand cDNA using reverse transcriptase and random primers. The RNA template removed and a replacement strand synthesized, incorporating dUTP in place of dTTP to generate ds cDNA. These cDNA fragments then had the addition of a single 'A' base and subsequent ligation of the adapter. The products were then purified and enriched with PCR to create the final cDNA library. Paired-end strand-specific RNA-seq
GEO
GSM3381519
GEO Accession
GSM3381519