Structural genome of the C. posadasii Silveira strain
The sequencing library was constructed from 6ug ofDNA following the Pacbio (Pacific Biosciences, USA) protocol for use with theSMRTbellExpress Template Prep Kit. The ligated library templates were size selected on Sage Blue Pippin instrument (Sage Science, USA) for selection of fragments of 17kb and larger. The final purified sequencing library yield was quantified to 980ng with a final mode size of 38.3kb as determined by Fragment Analyzer (Agilent Technologies, USA).The final library was bound with Pacbio polymerase and sequencing primer v3 using manufacturers methods.One 1M v2 SMRT cell was loaded with bound library at a loading concentration of 7pmol/cell followed by a 10-hoursequencing run on the Pacbio Sequel Instrument at the University of Arizona, Tucson AZ
SUB5914207
Sil_PacBio