GSM3927350: spp1 knockout 1st repeat 0 minutes after rapamycin; Saccharomyces cerevisiae BY4741; RNA-Seq
Pelleted cells were resuspended in TES (10 mM Tris pH 7.5, 10 mM EDTA pH 8, 0.5 % SDS). RNA was then extracted with phenol:chloroform followed by ethanol precipitation. 500 ng of RNA was used for library preparation with the Lexogen 3' end RNA-seq library prep kit.
GEO
GSM3927350