16S rRNA gene amplicon sequencing of groundwater
Groundwater was filtered on 0.2m filter. Residue was resuspended and DNA extracted using the Epicentre MasterPure DNA purification kit. Partial 16S rRNA gene amplicons were generated with the F515/R806 primers. Libraries were prepared using Ilumina Nextera XT v2 barcodes and sequenced on an Illumina MiSeq.
SUB7771892
V11-2