GSM4121429: Pto_col_48h_1; Pseudomonas syringae pv. tomato str. DC3000; RNA-Seq
For RNA extraction, the TriFast solution was mixed with 200 μl of chloroform and the aqueous phase was isolated by centrifugation (typically 400 μl). The aqueous phase was mixed with 200 μl (half volume) of ethanol and then applied to the column of RNAqueous kit (Ambion). RNA was eluted in 30 μl of RNase free water following the manufacture's protocol and treated with 2 U of TURBO DNase (Ambion) for 30 min at 37 ºC. Plant rRNAs were removed using Ribo-Zero plant kit (Epicentre) following the manufacture's protocol. cDNA libraries were generated with Ovation Complete Prokaryotic RNA-Seq kit 1-8 (NuGEN)
GEO
GSM4121429
GEO Accession
GSM4121429