GSM3536655: H1_plate1; Mus musculus; RNA-Seq
Cells were thawed on ice and each sample was lysed using 1uL of 1mg/mL Proteinase K (ThermoFisher Scientific) followed by incubation at 50oC for 15 min, then 95oC for 10 min uncovered to evaporate all liquid. Single Cell RNA Barcoding and Sequencing (SCRB-seq) was performed as described in Soumillon M., Cacchiarelli D., Semrau S., van Oudenaarden A. and Mikkelsen T.S. (2014). Characterization of directed differentiation by high-throughput single-cell RNA-Seq. bioRxiv. 10.1101/003236. Sequencing generates two files: read1 and read2.  Read1 contains 17 base reads (6 base barcode + 10 base UMI + an N in the 17th position).  Read2 contains the 46 bp cDNA sequence.
GEO
GSM3536655
GEO Accession
GSM3536655