Single-cell analysis reveals different age-related somatic mutation profiles between stem and differentiated cells in human liver: Sample L4-H4
"Single hepatocytes from each individual subject were subjected to whole genome amplification (WGA) using our modified procedure of low temperature cell lysis and DNA denaturation followed by multiple displacement amplification (MDA) as described in Dong X et al. Nature Methods. The libraries for Illumina next-generation WGS were generated from single cell MDA DNA human samples using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs). The libraries were sequenced with 2 x 150 bp paired-end reads on an Illumina HiSeq X Ten sequencing platform by Novogene, Inc."
AECM-phs001956
Lib_L4-H4