GSM2564724: MC2_H3K27ac; Mus musculus; ChIP-Seq
Cells were cross-linked with 1% formaldehyde, lysed, and sonicated. Sonicated DNA fragments that bound to H3K4me1 or H3K27ac were immunoprecipitated with anti-H3K4me1 antibody or anti-H3K27ac antibody, respectively. The immunoprecipitated DNA was repaired, ligated to adapters, and size selected with the Ion Plus Fragment Library Kit (#4471252, Thermo Fisher Scientific, Waltham, MA) according to the Ion ChIP-Seq Library Preparation protocol. Libraries were analyzed for size and quantity using the High Sensitivity Bioanalyzer Kit (#5067-4626, Agilent Technologies, Santa Clara, CA). The ChIP library was sequenced on an Ion Torrent Proton Semiconductor Sequencer.
GEO
GSM2564724
GEO Accession
GSM2564724