Illumina HiSeq 2000 paired end sequencing
The short-insert paired-end libraries were prepared with NO-PCR protocol. TruSeq™DNA Sample Preparation Kit v2 (Illumina Inc.) and the KAPA Library Preparation kit (Kapa Biosystems) were used. In short, 2.0 micrograms of sheared genomic DNA was end-repaired, adenylated and ligated to Illumina specific indexed paired-end adaptors. The DNA was size selected with AMPure XP beads (Agencourt, Beckman Coulter) in order to reach the fragment size of 220-550bp.
CNAG
ena-EXPERIMENT-CNAG-16-07-2019-14:04:17:239-2
Centro Nacional de Análisis Genómico
ena-EXPERIMENT-CNAG-16-07-2019-14:04:17:239-2