GSM2905291: BMPi-2; Homo sapiens; RNA-Seq
Organoids were minced into small pieces and then digested in 0.25% trypsin, 10 g/ml Dnase, 100 nM TTX, 10 μM DNQX, 50 μM DL-AP5 in HBSS at 37 °C for 20 min and stopped digestion with 100 μg/ml trypsin inhibitor. Single cell suspension was genetrated by pipetting for ~ 25 cycles and filtered through a 45 μm cell strainer. Cells were washed with PBS before counting and sorting. Library construction follows the manufactural standard protocol
GEO
GSM2905291
GEO Accession
GSM2905291