GSM4618899: SC340; Homo sapiens; RNA-Seq
Whole lung tissue was mechanically and enzymatically dissociated and filtered to create a single cell suspension.  Each patient sample was then cryopreserved as a single cell suspension.  Once selected, ten individual lung suspensions were thawed, incubated with a unique TotalSeq-C hashtag antibody and viability was assessed.  From each individual cell suspension, 3500 TotalSeq-C antibody hashtag-labeled cells were combined into one 10X Genomics 5Prime Immune Profiling reaction. The gene expression (GEX) and cell hashing/feature barcode (HTOFB) libraries were constructed according to the manufacturer's protocol, specifically the Chromium Single Cell V(D)J Reagent Kits User Guide (v1 Chemistry) with Feature Barcoding technology for Cell Surface Protein
GEO
GSM4618899
GEO Accession
GSM4618899