GSM2402717: Repo/TDP43:S23_small RNA; Drosophila melanogaster; ncRNA-Seq
All small RNA libraries were constructed from 15-20 ug of TRIzol isolated total RNA. 18-29 nucleotide long small RNAs were size selected on 15% PAGE-UREA gel. After 3’- and 5’-adapter ligation, subsequent gel purification steps and reverse transcription cDNAs were PCR amplified with barcoded primers. PCR products were size selected on 6% PAGE gel, and quantified on Bioanalyzer. Loading concentrations were determined using the NEBNext Library Quant kit for Illumina (NEB). The libraries were sequenced on illumina NextSeq 500 platform.
GEO
GSM2402717
GEO Accession
GSM2402717