Raw RNA-seq paired-end reads from Tissue Culture tissue, Nicotiana benthamiana
RNA-seq libraries for each tissue were prepared from total RNA using the Illumina RNAseq sample prep multiplex protocol (not strand specific) according to manufacturers instructions. Briefly, Poly-A mRNA were purified using oligo (dT) primed magnetic beads and chemically fragmented into smaller pieces. Cleaved fragments were converted to double-stranded cDNA using random hexamer primers. After purification and end-repair, the cDNA fragments were ligated to Illumina paired-end sequencing adapters which contain sample specific indexes to allow for sequencing of multiple libraries on a single lane of an Illumina flowcell. Following this, fragments were purified, size selected on a gel, and then amplified by PCR to obtain the final library. The libraries representing 9 tissues were then pooled in equal quantities into a single sample for sequencing on the Illumina HiSeq-2000 platform.
University of Sydney
Nicotiana benthamiana RNA-seq library, raw Tissue Culture read 1