Adult non-failing heart: 5hmC human cardiac myocyte
DNA was extracted from sorted cardiac myocyte nuclei using the AllPrep DNA/RNA Kit (Qiagen). DNA from biological replicates was pooled and sheared in a volume of 100  l at low intensity for 30 cycles (30 sec on/ 90 sec off) using a Bioruptor (Diagenode). Sheared DNA was processed using the hydroxymethyl Collector Seq Kit (Active Motif) according to manufacturer's instructions. The resulting DNA was used for library generation using the NEBNext Ultra DNA Library Prep Kit (NEB).
SUB3380142
NF_5hmC_seq