GSM3929167: WT_rep1; Triticum aestivum; RNA-Seq
Total RNA were extracted 180 mg of shoots of 12-day-old seedlings with the NucleoSpin RNA kit (Macherey-Nagel), according to the manufacturer's instructions. RNA-seq libraries were prepared using 2 μg of total RNA with the NEBNext® Poly(A) mRNA Magnetic Isolation Module and NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (New England Biolabs), according to manufacturer's recommendations. The quality of the libraries was assessed with Agilent 2100 Bioanalyzer (Agilent).
GEO
GSM3929167