GSM2666928: C1_1A_L1; Pseudomonas aeruginosa; RNA-Seq
The total RNA extraction was performed at 13 hours incubation, that is 2 hours after nanoparticle exposure. The bacteria suspension of 6 mL from each serum bottle was collected and centrifuged at 14,000 rpm for 5 minutes at 4 oC, the supernatant was discarded and the pellets were immediately frozen in the liquid nitrogen before storing at -80ºC for later RNA extraction. Total RNA extraction was performed using the QIAGEN miRNeasy Mini Kit (Catalog number: 217004) according to the manufacturer’s instructions except adding an extra beat-beading step to ensure the complete lysis of the bacterial cells. Strand-specific cDNA library construction
GEO
GSM2666928
GEO Accession
GSM2666928