Fungal ITS sequences from Lonar Lake sediment metagenome
Isolated metagenome are subjected for fungal tag-encoded FLX amplicon pyrosequence (fTEFAP). It is performed using ITS specific fungal primers. ITS1-F 5’CTTGGTCATTTAGAGGAAGTAA and  ITS4R 5’ TCCTCCGCTTATTGATATGC were employed to amplify 600-bp fragments of the fungal internal transcribed spacer (ITS) region. Tag encoded FLX amplicon pyrosequencing analysesis done using Roche 454 FLX instrument with Titanium reagents.  out put fna file was converted by standalone phred33 conversion tools
VNSG University
Lonar Lake Sediment fungal metagenome