DNA reads from mixed strains bacteria cultures
S. epidermidis strains NIHLM001 and NIHLM023 were grown in tryptic soy broth at 37C. After 4 hours of growth (OD600 ~ 0.2), both cultures were mixed and 125 g/ml of erythromycin was added (MICNIHLM001 = 4 mg/ml, MICNIHLM023 < 0.004 mg/ml). Growth continued for another 3 hours and we collected samples every hour post-antibiotics treatment for DNA library preparation and sequencing.
SUB4505556
erythromycin_time1