Microbiomes of I. campana, I. sp, and surrounding bacterioplankton
Samples were stored in ethanol, and DNA was extracted using the Qiagen DNeasy Blood and Tissue kit.  Sequenced at Molecular Research LP (Shallowater, Texas)on a MiSeq® sequencer (Illumina).  V4 region of the 16S rRNA gene was amplified using the bacterial and archaeal primers 515F and 806R   Metadata below (formatted in for a tab delimited text file)  #SampleID BarcodeSequence LinkerPrimerSequence BarcodeName Repeats ProjectName Description Sample Source Collection Site Long &n Lat Date Collected Depth  WW1 GAGAGTGT GTGCCAGCMGCCGCGGTAA 515Fbar1  121514CM515Fillumina WW1 seawater "Wilmington, North Carolina" "34° 7.926 N, 77° 21.636 W" 1-Jul-14 10-20m  WW2 GAGATCAG GTGCCAGCMGCCGCGGTAA 515Fbar2  121514CM515Fillumina WW2 seawater "Wilmington, North Carolina" "34° 7.926 N, 77° 21.636 W" 1-Jul-14 10-20m  WW3 GAGATCTC GTGCCAGCMGCCGCGGTAA 515Fbar3  121514CM515Fillumina WW3 seawater "Wilmington, North Carolina" "34° 7.926 N, 77° 21.636 W" 1-Jul-14 10-20m  GW1 GAGATGAC GTGCCAGCMGCCGCGGTAA 515Fbar4  121514CM515Fillumina GW1 seawater "Gray's Reef, Georgia" "31° 23.790 N, 80°53.418 W" 1-Aug-14 10-20m  GW2 GAGATGTG GTGCCAGCMGCCGCGGTAA 515Fbar5  121514CM515Fillumina GW2 seawater "Gray's Reef, Georgia" "31° 23.790 N, 80°53.418 W" 1-Aug-14 10-20m  GW3 GAGTACAG GTGCCAGCMGCCGCGGTAA 515Fbar6  121514CM515Fillumina GW3 seawater "Gray's Reef, Georgia" "31° 23.790 N, 80°53.418 W" 1-Aug-14 10-20m  PW1 GAGTACTC GTGCCAGCMGCCGCGGTAA 515Fbar7  121514CM515Fillumina PW1 seawater "Bocas del Toro, Panama" "9° 21.639 N, 82° 16.680 W" 2-Aug-14 10-20m  PW2 GAGTAGAC GTGCCAGCMGCCGCGGTAA 515Fbar8  121514CM515Fillumina PW2 seawater "Bocas del Toro, Panama" "9° 21.639 N, 82° 16.680 W" 2-Aug-14 10-20m  LW1 GAGTAGTG GTGCCAGCMGCCGCGGTAA 515Fbar9  121514CM515Fillumina LW1 seawater "Key Largo, Florida" "24° 56.657 N, 80° 29.768 W" 1-Jun-14 10-20m  LW2 GAGTCACT GTGCCAGCMGCCGCGGTAA 515Fbar10  121514CM515Fillumina LW2 seawater "Key Largo, Florida" "24° 56.657 N, 80° 29.768 W" 1-Jun-14 10-20m  LW3 GAGTCAGA GTGCCAGCMGCCGCGGTAA 515Fbar11  121514CM515Fillumina LW3 seawater "Key Largo, Florida" "24° 56.657 N, 80° 29.768 W" 1-Jun-14 10-20m  BS1 GAGTCTCA GTGCCAGCMGCCGCGGTAA 515Fbar12  121514CM515Fillumina BS1 unkown Ircinia sp "Wilmington, North Carolina" "34° 7.926 N, 77° 21.636 W" 1-Jul-14 10-20m  BS2 GAGTCTGT GTGCCAGCMGCCGCGGTAA 515Fbar13  121514CM515Fillumina BS2 unkown Ircinia sp "Wilmington, North Carolina" "34° 7.926 N, 77° 21.636 W" 1-Jul-14 10-20m  BS3 GAGTGACA GTGCCAGCMGCCGCGGTAA 515Fbar14  121514CM515Fillumina BS3 unkown Ircinia sp "Wilmington, North Carolina" "34° 7.926 N, 77° 21.636 W" 1-Jul-14 10-20m  PS1 GAGTGAGT GTGCCAGCMGCCGCGGTAA 515Fbar15  121514CM515Fillumina PS1 Ircinia campana "Bocas del Toro, Panama" "9° 21.639 N, 82° 16.680 W" 2-Aug-14 10-20m  PS9 GAGTGTCT GTGCCAGCMGCCGCGGTAA 515Fbar16  121514CM515Fillumina PS9 Ircinia campana "Bocas del Toro, Panama" "9° 21.639 N, 82° 16.680 W" 2-Aug-14 10-20m  PS3 GAGTGTGA GTGCCAGCMGCCGCGGTAA 515Fbar17  121514CM515Fillumina PS3 Ircinia campana "Bocas del Toro, Panama" "9° 21.639 N, 82° 16.680 W" 2-Aug-14 10-20m  PS4 GAGTTCAC GTGCCAGCMGCCGCGGTAA 515Fbar18  121514CM515Fillumina PS4 Ircinia campana "Bocas del Toro, Panama" "9° 21.639 N, 82° 16.680 W" 2-Aug-14 10-20m  WS3 GAGTTCTG GTGCCAGCMGCCGCGGTAA 515Fbar19  121514CM515Fillumina WS3 Ircinia campana "Wilmington, North Carolina" "34° 7.926 N, 77° 21.636 W" 1-Jul-14 10-20m  WS8 GAGTTGAG GTGCCAGCMGCCGCGGTAA 515Fbar20  121514CM515Fillumina WS8 Ircinia campana "Wilmington, North Carolina" "34° 7.926 N, 77° 21.636 W" 1-Jul-14 10-20m  WS2 GAGTTGTC GTGCCAGCMGCCGCGGTAA 515Fbar21  121514CM515Fillumina WS2 Ircinia campana "Wilmington, North Carolina" "34° 7.926 N, 77° 21.636 W" 1-Jul-14 10-20m  WS9 GATCACCA GTGCCAGCMGCCGCGGTAA 515Fbar22  121514CM515Fillumina WS9 Ircinia campana "Wilmington, North Carolina" "34° 7.926 N, 77° 21.636 W" 1-Jul-14 10-20m  GS10 GATCACGT GTGCCAGCMGCCGCGGTAA 515Fbar23  121514CM515Fillumina GS10 Ircinia campana "Gray's Reef, Georgia" "31° 23.790 N, 80°53.418 W" 1-Aug-14 10-20m  GS1 GATCAGCT GTGCCAGCMGCCGCGGTAA 515Fbar24  121514CM515Fillumina GS1 Ircinia campana "Gray's Reef, Georgia" "31° 23.790 N, 80°53.418 W" 1-Aug-14 10-20m  GS5 GATCAGGA GTGCCAGCMGCCGCGGTAA 515Fbar25  121514CM515Fillumina GS5 Ircinia campana "Gray's Reef, Georgia" "31° 23.790 N, 80°53.418 W" 1-Aug-14 10-20m  GS7 GATCCAAC GTGCCAGCMGCCGCGGTAA 515Fbar26  121514CM515Fillumina GS7 Ircinia campana "Gray's Reef, Georgia" "31° 23.790 N, 80°53.418 W" 1-Aug-14 10-20m  LS8 GATCCATG GTGCCAGCMGCCGCGGTAA 515Fbar27  121514CM515Fillumina LS8 Ircinia campana "Key Largo, Florida" "24° 56.657 N, 80° 29.768 W" 1-Jun-14 10-20m  LS4 GATCCTAG GTGCCAGCMGCCGCGGTAA 515Fbar28  121514CM515Fillumina LS4 Ircinia campana "Key Largo, Florida" "24° 56.657 N, 80° 29.768 W" 1-Jun-14 10-20m  LS1 GATCCTTC GTGCCAGCMGCCGCGGTAA 515Fbar29  121514CM515Fillumina LS1 Ircinia campana "Key Largo, Florida" "24° 56.657 N, 80° 29.768 W" 1-Jun-14 10-20m  LS10 GATCGAAG GTGCCAGCMGCCGCGGTAA 515Fbar30  121514CM515Fillumina LS10 Ircinia campana "Key Largo, Florida" "24° 56.657 N, 80° 29.768 W" 1-Jun-14 10-20m  CS8 GATCGATC GTGCCAGCMGCCGCGGTAA 515Fbar31  121514CM515Fillumina CS8 Ircinia campana "Carrie Bow Cay, Belize" "16° 48.173 N, 88°4.928 W" 1-Sep-14 10-20m  CS9 GATCGTAC GTGCCAGCMGCCGCGGTAA 515Fbar32  121514CM515Fillumina CS9 Ircinia campana "Carrie Bow Cay, Belize" "16° 48.173 N, 88°4.928 W" 1-Sep-14 10-20m  CS4 GATCGTTG GTGCCAGCMGCCGCGGTAA 515Fbar33  121514CM515Fillumina CS4 Ircinia campana "Carrie Bow Cay, Belize" "16° 48.173 N, 88°4.928 W" 1-Sep-14 10-20m  CS10 GATCTCCT GTGCCAGCMGCCGCGGTAA 515Fbar34  121514CM515Fillumina CS10 Ircinia campana "Carrie Bow Cay, Belize" "16° 48.173 N, 88°4.928 W" 1-Sep-14 10-20m
UNCW
Ircinia campana project