Illumina Genome Analyzer II sequencing; microRNA-Seq of Ras induced Epithelial to Mesenchymal Transition (EMT) in Madin-Darby Canine Kidney (MDCK) cells
microRNA-Seq of Ras induced Epithelial to Mesenchymal Transition (EMT) in Madin-Darby Canine Kidney (MDCK) cells
MDCK and MDCK-Ras cells were cultivated in DMEM/F12 +10%FCS. Confluent cultures were used for the experiment. Total RNA was isolated from 4 biological replicates from each cell type (MDCK and MDCK-Ras) using peqGOLD TriFast (Peqlab) according to the manufacturer’s instructions. Quality and quantity of total RNA was checked on Agilent Bioanalyzer according to manufacturer’s instructions. Libraries for sequencing were prepared using Illumina Small RNA Sample Prep kit v1.5 (Illumina, San Diago, CA USA) as described in the corresponding protocol. Amplified libraries were size fractionated on a 2 % low-melt agarose gel and fragments with a length between 90 to 110 nucleotides were excised. Eluated template libraries were quantified and quality checked using Qubit dsDNA HS Assay Kit (Invitrogen) and on an Agilent Bioanalyzer (Agilent).
INSTITUTE OF ANIMAL BREEDING & GENETICS, UNIVERSITY OF VETERINARY MEDICINE VIENNA
E-MTAB-3302:Mueller_Lab_Feb2015_MDCK_miRNA_biorep4
Institute of Animal Breeding & Genetics, University of Veterinary Medicine Vienna
E-MTAB-3302:Mueller_Lab_Feb2015_MDCK_miRNA_biorep4
Experimental Factor: phenotype
epithelial