GSM4672923: Yap_active_RNAseq4; Mus musculus; RNA-Seq
RNA and Chromatin was isolated from sorted EPCAM+ lung epithelial cells as follows: AT2 cells were isolated using MACs magnetic bead (Miltenyi Biotec) selection of CD45 (BIOLEGEND), CD16/32 (Fisher), Ter119 (BIOLEGEND), CD90.2 (BIOLEGEND), CD271 (Miltenyi Biotec), and CD31 (BIOLEGEND) negative cells followed by positive selection of CD326 (Miltenyi Biotec) positive cells. Samples were split with 50,000 cells being used for ATACseq library  prep, while remaining cells were used for RNA isolation via Qiagen RNAeasy minikit with on-column DNAse digestion. RNAseq: Libraries were prepared with Illumina RNA PolyA selection, ATAC libraries were prepared using Illumina Nextera XT library kit RNAseq: 20-30 million reads ; Illumina HiSeq 2x150 bp sequencing, ATACseq: 50 million reads ; Illumina HiSeq 2x150 bp sequencing
GEO
GSM4672923
GEO Accession
GSM4672923