Illumina HiSeq 2500 sequencing; The effect of Dis3 knockdown on miRNA expression in Drosophila wing imaginal discs.
The effect of Dis3 knockdown on miRNA expression in Drosophila wing imaginal discs.
60 wing imaginal discs per sample were dissected from wandering 3rd instar larvae. Nubbin-Gal4 homozygous females were crossed to homozygous UAS-Dis3RNAi males at 25 degrees C.All offspring were of knockdown (nubbin-Gal4/UAS-Dis3RNAi) genotype. RNA was extracted from each sample of 60 wing discs using a miRNeasy kit. RNA was size selected (18-22nt) then libraries were constructed using illumina TruSeq Small RNA sample preparation protocol.
BRIGHTON AND SUSSEX MEDICAL SCHOOL
E-MTAB-3569:UAS-Dis3RNAi 1
Brighton and Sussex Medical School
E-MTAB-3569:UAS-Dis3RNAi 1
Experimental Factor: phenotype
Wild type
Experimental Factor: RNA interference
Control construct -  v35090 from Vienna Drosophila RNAi centrer