Whole-exome sequencing of 1 member of a pedigree with lumbar disc degeneration
Genomic DNA was isolated and further fragmented using Covaris S220. The fragmented DNA underwent end repair and A-tailing and adapter ligation. Subsequently, pre-capture LM-PCR was used for enrichment and Roche NimbleGen EZ library capture was performed. Following post-capture LM-PCR, the library was constructed.
Department of Orthopaedics, Xijing Hospital, Fourt
WES_UE_41