daf-2(e1370); daf-16(mu86); Punc-119::gfp--sorted neurons rep 1
RNA-seq was performed on daf-2;dfa-16 neurons isolated from Day 1 adult C. elegans. Total RNA from FACS-isolated, daf-2;daf-16;Punc-119::gfp labeled neuron samples was isolated and amplified using the NuGen Ovation RNAseq v2 kit. Amplified cDNA was sheared to 200bp fragments. Library was prepared using the Illumina TruSeq DNA Sample Prep kit.
Princeton University
daf-2(e1370);daf-16(mu86) neurons replicate 1