Colorectal mucosal microbiota on pCLE imaged sites
The biopsied mucosal samples was transferred to Majorbio (Shanghai, China) where total DNA was extracted, amplified and sequenced according to their standardized protocol. he V3->V1 region of 16S ribosomal subunit gene was amplified using 27F/533R barcoded primers and sequenced using Roche Genome Sequencer FLX+).The barcode and primer sequence was NOT removed from the sff file.
Shandong University, Qilu Hospital
sop1.DLW0114N_CTACGATATG.TTACCGCGGCTGCTGGCAC.sff