GSM3537391: 5C_B38_LPi1_LinxP-inv_mut1; Mus musculus; OTHER
For Chromosome Conformation Capture (5C and Capture-C) cells were fixed in culture plates or flasks and scraped off. For RNA-seq cells were washed once with PBS and lysed in Trizol in the culture plate. For ATAC-seq, cells were washed once with PBS and directly incubated in the transposition reaction. 5C libraries were generated based on a custom Illumina-compatible protocol (Nora et al 2017); Capture-C libraries were generated using KAPA Hyper Prep Kit (KK8500, Kapa Biosystems); RNA-seq libraries were generated using the TruSeq Stranded Total RNA kit (Illumina); ATAC-seq libraries were generated based on a Nextseq-compatible protocol (Buenrostro et al 2013).
GEO
GSM3537391
GEO Accession
GSM3537391