16S sequencing of human gut metagenome:BIS_405
DNA extracted by Qiagen PowerSoil Cat#12888-100.V4 region targeted by primers 515F and 806R.PCR design: primary 94C/3min, (94C/45s, 55C/60s, 72C/90s)x20, 72C/10min;secondary as per primary except 25 cycles after 1:10 dilution.Samples barcoded per 8-base Illumina Nextera design.Sequencing via paired end 600-cycle (2x311) kit on MiSeq.
SUB6423662
BIS_405