Ascosphaera apis mycelium
Total RNA of Ascosphaera apis mycelium sample isolated using TRizol reagent (Thermo Fisher, Shanghai, China) on dry ice and thenreversely transcribed using Maxima H Minus Reverse Transcriptase, followed by purification and concentration on a AMPure XP beads. Secondly, cDNA librarieswere constructed from 50 ng total RNA with a 14 cycles PCR using the cDNA-PCR Sequencing Kit (SQK-PCS109) and PCR Barcoding Kit (SQK-PBK004) followingthe manufacturer's protocol (Oxford Nanopore Technologies Ltd, Oxford, UK). Thirdly, the aforementioned cDNA libraries were sequenced by BiomarkerTechnologies (Beijing, China) utilizing PromethION system (Oxford Nanopore Technologies Ltd, Oxford, UK).
SUB7774160
AaM