GSM3984860: Control rep1; Homo sapiens; ATAC-seq
We conducted ATAC-seq as described in Buenrostro et al. (Curr. Protoc. Mol. Biol. 2015) using a home-made Tn5 that we synthesized as described in Picelli et al. (Genome Res. 2014). For each replicate we incubated 250,000 cells with 12.5 uL of 1:1 mix of Tn5 that carry 5-methylC-MEDS-A oligos and MEDS-B oligos at 37℃ for 30 minutes in a 50 uL reaction. We column-purified the tagmented DNA using the Zymo DNA Clean & Concentrator-5 kit (Zymo Research, Irvine, CA). We constructed Illumina sequencing library using the Kitzman lab custom index primers. We PCR-amplified a total of 11 cycles (5 initial + 6 additional) until amplification curve reached its mid-log phase (⅓ to ½ of max signal), and then purified the products using SPRI beads prepared as in (Rohland and Reich, Genome Res. 2012) and eluted in 22 uL of TTE8 buffer.
GEO
GSM3984860
GEO Accession
GSM3984860