GSM4278053: BT549_SOX4-gRNA2-UT-2; Homo sapiens; RNA-Seq
Total RNA was extracted from control, SOX4 or ITGAV edited human BT549 and murine 4T1 cells cultured in complete RPMI media in biological triplicates.  RNA extraction was performed using the RNeasy Plus Mini Kit (Qiagen # 74134) following the manufacturer's protocol. Total RNA was quality-checked using an Agilent BioAnalyzer 2000 instrument.  RNA with an integrity number of greater than 9.5 was used for subsequent analyses. Libraries were prepared with TruSeq RNA Sample Prep Kit v2 (Illumina). Library concentrations were quantified by Qubit (Invitrogen) and mixed equally for single-end 75bp sequencing using an Illumina NextSeq 500 instrument.
GEO
GSM4278053
GEO Accession
GSM4278053