Bisulfite Sequencing of the Sox1 promoter in Mus musculus Neueal progenitor cells: cells with targeted Sox1 activation and Dnmt inhibition, unsorted; replicate 2
Total DNA was isolated using the  DNeasy Blood and Tissue kit (Quiagen, 69504). DNA was bisulfite converted using the EZ DNA Methylation-Gold Kit (Zymo, D5005), and the Sox1 promoter region was amplified via PCR. Sequencing adapters were added with the Nextera DNA Library Preparation Kit (Illumina, FC-121-1012).
SUB4501255
VB_BiS18