Illumina HiSeq 4000 paired end sequencing
Filters were stored at –80 °C until extraction. DNA was extracted from the membranes according to Boström et al. This included overnight lysis, phenol chloroform extraction, and coprecipitation with GlycoBlue™ (ThermoFisher). DNA concentration was quantified by Qubit™ (ThermoFisher).
Libraries were prepared using a Nextera XT DNA Library kit (Illumina)
J CRAIG VENTER INSTITUTE
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