GSM2564463: Croatan_dual_screen_A375_T0_R3; synthetic construct; OTHER
Genomic DNA was extracted 2 days after infection (T0) and after 12 doublings (T6) using the QIAamp Blood DNA Midi Kit (Qiagen). For each sample, sgRNA sequences were extracted from genomic DNA in 8 separate 30-cycle PCR reactions where 2 ug of input DNA was included in each reaction. Illumina adapters where included on the PCR primers for this PCR. Samples were sequenced on the Illumina MiSeq platform.
GEO
GSM2564463
GEO Accession
GSM2564463