Comprehensive Long Span Paired-End-Tag Mapping Reveals Recurrent Fusion Genes in Gastric Cancer Genomes
100 ng of genomic DNA from the tumor and paired normal samples were fragmented to 300 bp with a Covaris S-2 instrument (Covaris, Woburn, MA, USA). The exome captured libraries were prepared with NimbleGen SeqCap EZ v3.0 (Roche NimbleGen Inc.) by following the manufacturers’ protocols. Sequencing was performed on Illumina HiSeq 2000 by 2 x 101 bases.
Genome Institute of Singapore
CHH7966_Normal