GSM3384416: iOL_Seh1_ChIP-Seq; Rattus norvegicus; ChIP-Seq
Briefly, for ChIP-Seq, approximately 5 x10^6 cells were cross-linked with 1% formaldehyde for 10 min at room temperature and then were quenched with 125 mM glycine for 5min. Sonicated chromatin was used for immunoprecipitation by incubation with appropriate antibodies (4 g) overnight at 4°C. Immunoprecipitated complexes were collected using protein 40l A/G plus agarose beads (Millipore). SubSequently, beads were washed Sequentially with low-salt buffer, high-salt buffer, LiCl buffer and twice with TE and elution in 500 l of elution buffer (1% SDS, 0.1M NaHCO3). The elutes were heated at 42°C for 2h and treatment with proteinaseK followed by 65°C 10 h to reverse the cross-linking and were treated  with RNaseA for 30 min before DNA was extracted and purified. The ChIP libraries were prepared using KAPA HyperPrep Kits (Roche, 7962347001)
GEO
GSM3384416
GEO Accession
GSM3384416