GSM2827757: rel- relAE335Q replicate 1_dark_15 min; Synechococcus elongatus PCC 7942; RNA-Seq
Frozen cells were resuspended in the AE buffer with external RNA standards and lysed at 65° C with phenol/SDS by vortexing. Total RNA was extracted with phenol/chloroform followed by isopropanol precipitation. Then, total RNA was subjected to DNase I (Promega) treatment followed by phenol/chloroform extraction and ethanol precipitation. To assess integrity total RNA was analyzed on an agarose gel. Ribosomal RNA was depleted from total RNA using the Ribo-Zero rRNA removal Gram-negative bacteria kit (Illumina), following the protocol provided by the manufacturer. RNA-sequencing libraries were prepared using the TruSeq Stranded mRNA Sample Prep Kit (Illumina) from rRNA-depleted RNA. Samples were multiplexed and sequenced on an Illumina HiSeq 2000 platform by the Bauer Core Facility at the Harvard FAS Center for Systems Biology.
GEO
GSM2827757
GEO Accession
GSM2827757