GSM2667731: at-endosperm-RNAseq_replicate1_ler_wt_x_col_osd1; Arabidopsis thaliana; RNA-Seq
For RNA sequencing, endosperm from seeds derived from indicated crosses were dissected in biological duplicates. RNA was extracted following a modified protocol for the RNAqueous kit (Ambion, Life Technologies). RNA was purified by Qiagen RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) after residual DNA was removed by adding 2 uL DNaseI (Thermo-Scientific, Waltham, USA). Libraries were prepared using the TruSeq RNA Library Prep Kit v2 (Illumina, San Diego, USA) and sequenced in 50-bp single-end mode.
GEO
GSM2667731
GEO Accession
GSM2667731