GSM4125337: B73 Damage Replicate 3; Zea mays; RNA-Seq
Total RNA was isolated with the NucleoSpin® RNA Plant Kit (Takara Bio USA) according to the manufacturer's protocol. RNA quality was assessed based on RNA integrity number (RIN) using an Agilent Bioanalyzer. 3' RNA-seq library construction and sequencing were performed at Cornell University's Genomics Facility at the Institute of Biotechnology (Ithaca, NY, USA; http://www.biotech.cornell.edu/brc/genomics-facility/services). Approximate 500 ng of total RNA was used to construct the 3′ RNA-Seq libraries using the QuantSeq 3′ mRNA-Seq Library Prep Kit FWD (Lexogen, USA) according to the manufacturer's instructions. All libraries, each with their own unique adapter sequences, were pooled together and sequenced on one lane of an Illumina NextSeq 500 to generate about 90 bp single-end reads.
GEO
GSM4125337
GEO Accession
GSM4125337