Illumina HiSeq 2000 sequencing; RNA content of transcription factories
RNA content of transcription factories
Cell were treated with the pro-inflammatory cytokine TNFa for 0 or 30 min. Short and long RNA fractions were separated and DNase-treated after cell fractionation starting with about 1.5x10e7 cells using the miRNeasy mini kit (qiagen). RNA fraction were transformed into cDNA library with solexa adaptors using the standard Illumina protocol. Libraries were prepared using the NEB library prep kit for short RNAs.
GENOME ORGANIZATION AND FUNCTION GERMAN CANCER RESEARCH CENTER (DKFZ)
E-MTAB-3020:Total RNA 3 -  0 min
Genome organization and Function German Cancer Research Center (DKFZ)
E-MTAB-3020:Total RNA 3 -  0 min
Experimental Factor: stimulus
0 min TNF alpha
Experimental Factor: RNA
Total RNA
LIBRARY_STRAND
first strand