GSM1518042: Ribo-Seq Mettl3-KO ESCs; Mus musculus; RNA-Seq
Cells were lysed with 0.5% Triton, and the lysate was cleared by centrifugation, treated with RNase I for 45min and then loaded on a sucrose cushion. RNA was then isolated from cells using the acid:phenol extraction method. Small RNA fragments were size selected via gel purification, dephosphorylated, ligated, and reverse transcribed. The resulting cDNA was circularized and PCR amplified.
GEO
GSM1518042
GEO Accession
GSM1518042