GSM4672136: P8_NT_12hrs; Homo sapiens; RNA-Seq
Each explant was transferred to a 1.5 mL microcentrifuge tube, lysed in QIAzol reagent and homogenized in a TissueLyser LT (Qiagen) for 5 minutes at 50 Hz, using one 3.2 mm stainless steel bead per tube. mRNA was extracted using the miRNeasy Mini Kit (Qiagen) following the manufacturer's recommended protocol. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO
GSM4672136
GEO Accession
GSM4672136