GSM2667773: neu14_nuc_rep2; Homo sapiens; RNA-Seq
Libraries were prepared using the Illumina TruSeq Stranded Total RNA kit using 100ng RNA input and 13 PCR cycles. Polysomal RNA was extracted from sucrose gradient fractions by ethanol precipitation. RNA was then DNase treated, subjected to acid phenol:chloroform extraction, and ethanol precipitated. Cytoplasmic and nuclear RNA was extracted using TRIzol extraction (Life Technologies) and ethanol precipitation. Total RNA integrity was verified using a BioAnalyzer (Agilent).
GEO
GSM2667773
GEO Accession
GSM2667773