GSM3609619: Control rep2: MVBG233C; Mus musculus; RNA-Seq
Testes were removed and decapsulated. They were then digested with collagenase B (2mg/mL) for 10 minutes at 37°C. After rinsing in PBS, another digestion with 0.25% trypsin-EDTA for 5 minutes at 37°C was performed. The resulting cell suspension was washed twice, resuspended in sterile phosphate buffer solution (PBS) with DNase (50μg/mL) and filtered (40μm). Spermatogonia were isolated by fluorescence-activated cell sorting of Oct4-GFP positive germ cells with a FACSAria III laser sorter. Total RNAs from control and Kdm1a-cKO isolated PND 6 spermatogonia (n = 4 for each group) were extracted using the PicoPure RNA isolation kit (Arcturus, Burlington, ON, Canada). A total of 250 ng of each sample was used to prepare libraries using the TruSeq Stranded mRNA Sample Prep Kit (Illumina, San Diego, CA, USA) following the manufacturer's instructions.
GEO
GSM3609619
GEO Accession
GSM3609619