GSM2907057: ChIP replicate 2; Homo sapiens; ChIP-Seq
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. The libraries were prepared by the sequencing platform of the 'Centre National de Génotypage' (CEA, Evry, France) according to manufacturer recommendation using the NextFlexTM ChIP-sequencing kit (5143-01, BiooScientific, US) and adaptors NextFlexTM ChIP-seq barcodes (514120, BiooScientific, US). Twenty nanograms of ChIP and Input DNA were used as starting material, DNA extremities repaired and the resulting DNA PCR amplified (11-12 cycles). DNA materials were then size-selected and purified using agencourts AMPure XP magnetic beads (A63881, Beckman Coulter, US) prior to analysis on a Illumina Hi-seq 2000.
GEO
GSM2907057
GEO Accession
GSM2907057