Illumina HiSeq 4000 sequencing; Genome-wide Functional Annotation of Regulatory Elements in Livestock Species
Single-end ChIP-seq experiment targeting H3K4me3 in pig spleen on an Illumina HiSeq 4000
ftp://ftp.faang.ebi.ac.uk/ftp/protocols/assays/UCD_SOP_ChIP-Seq_Animal_tissue_20161101.pdf
UC DAVIS
ucd_spl_p348_h3k4me3
assay type
ChIP-seq
sample storage
frozen, -70 freezer
sample storage processing
cryopreservation in liquid nitrogen (dead tissue)
sampling to preparation interval
33
experimental protocol
ftp://ftp.faang.ebi.ac.uk/ftp/protocols/assays/UCD_SOP_ChIP-Seq_Animal_tissue_20161101.pdf
extraction protocol
ftp://ftp.faang.ebi.ac.uk/ftp/protocols/assays/UCD_SOP_ChIP-Seq_Animal_tissue_20161101.pdf
library preparation location
UC Davis, Meyer Hall
library preparation location longitude
-121.7405
library preparation location latitude
38.5449
library preparation date
2017-12-11
sequencing location
UC Davis Sequencing Center
sequencing location longitude
-121.7405
sequencing location latitude
38.5449
sequencing date
2017-12-11
experiment target
H3K4_trimethylation
chip protocol
ftp://ftp.faang.ebi.ac.uk/ftp/protocols/assays/UCD_SOP_ChIP-Seq_Animal_tissue_20161101.pdf
chip antibody provider
Diagenode
chip antibody catalog
C15410003
chip antibody lot
A1051D
library generation max fragment size range
200
library generation min fragment size range
200