GSM4061945: Human atrial tissue sample in age group SR60, replication 2 [mRNA]; Homo sapiens; RNA-Seq
The total RNA was extracted from the frozen human right atrial appendages using TRIZOL reagent following the manufacturer's protocol. The RNA yield and purity were determined using Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, USA) and NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific, Waltham, USA). For transcriptomic profiling, twelve human right atrial tissues were subjected to mRNA-seq according to the BIOPIC recommended workflow for mRNA-seq. A proportion (~2.5 μg) of twelve isolated total RNA samples was used to generate cDNA libraries using Illumina TruSeq RNA Sample Preparation v2 Kit (Lot # RS-122-2001, RS-122-2002). Each library was sequenced in one or two lanes (see below)
GEO
GSM4061945
GEO Accession
GSM4061945