HuR_HeLa_RNA-Seq
HeLa cells were grown in RPMI1640 containing 10% FBS and 1% penicillin streptomycin. To conduct HuR knockdown, cells were transfected with  100nm Dharmacon Smartpool J-003773-08 using Lipofectamine 2000. RNA levels were quantified using Qiagen QuantiTect Primer Assay QT00037856 with RPLPO as the endogenous control. RNA was purified using Qiagen RNeasy Kit and quality accessed with Agilent Bioanalyzer. RNA (10ug) was processed for sequencing using Illumina mRNA-Seq per protocol. Clusters were generated on the Illumina cluster station. Samples were run on an Illumina GAIIx 2 x 36 paired end read and CASAVA 1.6 was used to analyze and generate the raw reads.
University of Southern California
HeLa_HuR