ELU0236 16s v3-v5: Sample T587-S-NI
Samples were collected from subjects in the three following categories: 1.) ileal CD, 2.) IBD colitis without ileitis or any small bowel involvement, 3.) without IBD. A minimum of 4 ex-vivo biopsies were taken from fresh pathological specimens using Radial Jaw4 large capacity biopsy forceps, immediately placed in RNA stabilization solution. Amplicons of the V3-V5 hypervariable regions of the bacterial 16S rRNA gene were sequenced using the 454 FLX Titanium Sequencing Platform and the same primers employed for characterizing the microbial communities in healthy individuals at different body sites including the gastrointestinal tract by the Human Microbiome Project. Library construction and sequencing were performed at the Genome Institute at Washington University-St. Louis, Frank laboratory (UC Denver) and the Centre for Applied Genomics at the Hospital for Sick Children in Toronto, Canada.
NIDDK-phs000255
ELU0236_T587-S-NI_v3v5