GSM1701096: NoDice(4-25)ΔPKR cells transfected with vector control and pcDNA-3476; Homo sapiens; ncRNA-Seq
Total small RNA was directly harvested and isolated with the mirVana miRNA isolation kit (Ambion) All small RNA input was processed with the TruSeq Small RNA Sample Preparation Kit (Illumina). Adapter-ligated RNA was reverse transcribed using SuperScript III (Life Technologies) and amplified using GoTaq green PCR master mix (Promega) with TruSeq 3′ indices.  DNA bands corresponding to ~145 bp were isolated by gel electrophoresis on 10% polyacrylamide gels.
GEO
GSM1701096
GEO Accession
GSM1701096