RNA-Seq of Crassostrea virginica: mantle tissue
Total RNA from mantle tissue was extracted using TRI Reagent (MilliporeSigma, Burlington MA, U.S.A) and purified using DNA-free DNase treatment (Invitrogen, Waltham MA, U.S.A) following the manufacturers' protocols. Tagged libraries were constructed according to the Illumina TruSeq Stranded mRNA (Illumina, San Diego CA, U.S.A.) protocol, pooled, and paired-end sequenced on an Illumina HiSeq 2500 instrument with the standard v4 2 x 125 kit.
SUB6546212
DP57