GSM4671488: NTTSA-2E_ChIP; Mus musculus; ChIP-Seq
The zona pellucidae of the embryos were removed with 0.5% pronase E (Sigma) and the embryos were then incubated in Ca2+-free CZB medium for 10 -20 min. Polar bodies were removed by gentle pipetting using a fire-polished glass needle.  The zygote, 2-cell early, 2-cell late and morula stage wild- type embryos were collected at 13-14, 20-21, 33-34, 67-68 h.p.f. respectively. The 1-cell, 2-cell early, 2-cell late and morula stage SCNT and TSA treated SCNT embryos were collected at 13-14 h, 20-21 h, 33-34h, 67-68 h after activation, respectively. The zygote, 2-cell early, 2-cell late and morula stage wild- type embryos were collected at 13-14, 20-21, 33-34, 67-68 h.p.f. respectively. The 1-cellzygote, 2-cell early, 2-cell late and morula stage SCNT and TSA treated SCNT embryos were collected at 13-14 h, 20-21 h, 33-3 4h, 67-68 h after activation, respectively.
GEO
GSM4671488
GEO Accession
GSM4671488