GSM4121503: RNA-Seq: DOT1L_KO_Naïve B cells (CD43-) Replicate 2; Mus musculus; RNA-Seq
MACS sorted CD19+ cells were resuspended in Trizol (Ambion Life Technologies) and total RNA was extracted according to the manufacturer's protocol. Library preparation was performed on RNA samples with a measured RNA Integrity Number (RIN) between 8.0 and 10.0. Strand-specific libraries were generated using the TruSeq Stranded mRNA sample preparation kit (Illumina Inc.) according to manufacturer's instructions (Illumina, Part # 15031047 Rev. E). RNA libraries were prepared for sequencing using standard Illumina protocols.
GEO
GSM4121503
GEO Accession
GSM4121503