Amplicons were generated using one-step RT-PCR with primer pairs containing Illumina sequence tags and designed to tile the genome two times. Amplicons were pooled and indexed, PE libraries were prepared by amplifying the pool with primers containing index sequence and the rest of the Illumina adapter sequence.
UMIGS-GRC Illumina Construction Protocol Details
UMIGS
IL100098205_M00708_20180129_L1_experiment