Illumina of bacteria and fungi: grass sample
the V3-V4 region of the 16S rRNA gene and ITS1 region of the 5.8S gene  were amplified for bacteria and yeasts respectively.  In order to conduct multiplexing of samples within a single sequencing run and to assign sequences to samples in bioinformatics analysis, a specific tag was added to both forward and reverse primers. PCR products were purified through Agencourt AMPure XP beads (Beckman Coulter, Indianapolis, USA) according to manufacturers instructions and combined in equal molar concentration to generate a library pool. Both 16S and 5.8S rRNA gene libraries were sequenced at CIBIO (Center of Integrative Biology)  University of Trento (Trento, Italy).
SUB6534454
G45