Tn-seq of A. actinomycetemcomitans: growth in rich media under anoxic conditions (biological replicate 2)
TdT/2-PCR Tn-seq method: Purified DNA was sheared, poly-C-tailed with the TdT enzyme, amplified for 10 cycles with primers targeting the Tn10 transpson and C-tail (this primer builds part of the Illumina 3' adapter), amplified for 15 cycles with primers targeting the Tn10 transposon (downstream of the first primer; this primers builds the Illumina 5' adapter) and the 3' Illumina adapter (this primers builds the rest of the Illumina 3' adapter)
SUB1333650
Anox-2