GSM2663103: 10X38_2_GTCACCTGGCGTAT; Mus musculus; RNA-Seq
Mice were sacrificed by an overdose of Isoflurane and Ketamine/Xylazine, followed by   transcardial  perfusion  through  the  left  ventricle  with  artificial  cerebrospinal  fluid  (aCSF)   equilibrated in 95%O2 5%CO2 before use. The brain was removed and CA1 was microdissected   from  300μm  vibratome  sections.  Single‐cell  suspensions  were  prepared  using  Papain   (Worthington)  with  30min  enzymatic  digestion,  followed  by  manual  trituration  with  fire‐  polished Pasteur pipettes. The albumin density gradient was only performed for the p63 samples.   On  a  BD  FACSAria  II,  tdTomato‐positive  cells  were  sorted  into  oxygenated  aCSF  at  4°C,   concentrated, inspected for viability, and counted. Sorted  suspensions  were  added  to  10X  Chromium  RT  mix  aiming  at  2500  cells  recovered  per   experiment. Downstream cDNA synthesis (14 PCR cycles) and library preparation were carried   out  as  instructed  by  the  manufacturer  (10X  Genomics  Chromium  Single  Cell  Kit  Version  1).   Libraries were sequenced on the Illumina HiSeq2500 to an average depth 112 000 reads per cell   (raw),  yielding  on  average  3600  distinct  molecules  and  1700  genes  per  cell.
GEO
GSM2663103
GEO Accession
GSM2663103