Skud-RNA-rep1-pA
Total RNA was prepared by bead-beating cells with acid phenol / chloroform and precipitation. PolyA RNA was selected using oligo-dT beads. RNA was fragmented and reverse-transcribed with a primer containing illumina library priming sites and ending with oligodTV. The resulting cDNA was circularized and PCR amplified to generate barcoded Illumina libraries. For more see Yoon et al., RNA 2010 and Spealman et al., Meth Mol. Biol 2016).
SUB2779122
Sk-pA-rep1