RNA-Seq of Homo sapiens: HUVEC treated with FK506/Tacrolimus
HUVECs were isolated from anonymous umbilical veins and subcultured in 5% fetal bovine serum (FBS)-containing EC growth medium (Sciencell). HUVECs were rinsed with PBS and processed for RNA extraction using the RNeasy mini kit (Qiagen) according to the manufacturer’s instructions. Total RNA quality was verified using Thermo Scientific NanoDrop and Agilent Bioanalyzer. RNA was processed for RNA-Seq at the Genomics Resources Core Facility, Weill Cornell Medical College, New York, NY. Briefly, cDNA conversion and library preparation were performed using the TrueSeq v2 Illumina library preparation kit, following manufacturers’ recommended protocols. Samples were multiplexed 6 per lane and sequenced on an Illumina HiSeq4000 instrument.
SUB2559287
PM2-13-HUVEC-FK506-Batch2