GSM4672505: dG-2; Bacillus subtilis; RNA-Seq
Total RNA was extracted from collected cell paste via RNeasy mini-kit (Qiagen, cat # 74104) total RNA was dephosphorylated using CIP (NEB, discontinued), rRNA was depleted via ribo-zero Gram-positive rRNA depletion kits (Illumina, discontinued), remaining RNA was ligated to custom unique 2', 3' dideoxy RNA oligonucleotide (IDT), Illumina sequencing libraries were generated from ligation products using TruSeq Stranded mRNA kit (Illumina, cat # 20020594) Term-seq
GEO
GSM4672505
GEO Accession
GSM4672505