rummelc_2020
DNA was extracted using the Nucleo Spin Soil kit by Macherey. 16S rRNA gene V4 region was PCR amplified using the Illumina primer pair F515/R806 with the forward 5-GTGCCAGCMGCCGCGGT-3 and reverse 5-ACTACHVGGGTWTCTAAT-3 sequence (Caporaso et al., 2011) followed by Illumina MiSeq sequencing according to the Illumina  library preparation workflow described in the 16S Metagenomic Sequencing Library Preparation 184 library preparation workflow described in the 16S Metagenomic Sequencing Library Preparation 185 protocol
SUB7547016
aPET3