Illumina HiSeq 2000 sequencing; RNA-seq study of sexual reproduction in the diatom Skeletonema marinoi induced by a change in salinity
RNA-seq study of sexual reproduction in the diatom Skeletonema marinoi induced by a change in salinity
Cultures were collected by filtration onto 1.2 µm pore-size membrane filters (RAWP04700 Millipore) RNA was extracted with Trizol™ (Invitrogen) according to manufacturer's instructions Single-end (SE) libraries were prepared using a Beckman Biomek FX and the Illumina® TruSeq® Stranded Total RNA Sample Preparation kit, with poly-A selection and starting with 500 ng total RNA.
Stazione Zoologica Anton Dohrn of Naples
E-MTAB-8091:CS12_T1_s
Experimental Factor: cell type
sexual small cell
Experimental Factor: environmental stress
salinity shift
Experimental Factor: time
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