Illumina HiSeq 2000 sequencing; RNA-Seq of murine total lung RNA samples after AAV6.2-mediated Il13 overexpression
RNA-Seq of murine total lung RNA samples after AAV6.2-mediated Il13 overexpression
0.9x10^11 vg of AAV6.2 vectors containing the murine Il13 cDNA were administered intratracheally to Balb/c mice (9-12 wk). As a control, AAV6.2 vectors containing non-coding “stuffer DNA” were used. To assess vector-mediated effects following AAV6.2 administration, expression in AAV-stuffer treated animals was further compared to mice treated with PBS. cDNA libraries were prepared using the TruSeqTM RNA Sample Preparation Kit (# RS-122-2002, Illumina), low throughput protocol according to Illumina’s instructions
BOEHRINGER INGELHEIM PHARMA GMBH & CO. KG, DRUG METABOLISM AND PHARMACOKINETICS
E-MTAB-2598:203_15_PBS
Boehringer Ingelheim Pharma GmbH & Co. KG, Drug Metabolism and Pharmacokinetics
E-MTAB-2598:203_15_PBS
Experimental Factor: compound
phosphate buffered saline