GSM4125311: Gills, HSFchip; Crassostrea gigas; ChIP-Seq
Lysates were clarified from sonicated nuclei and HSF1-DNA complexes were isolated with antibody. The sample concentration was measured using the Qubit Fluorometer, and the fragment size and distribution were detected using the Agilent 2000. The 3'–5' exonuclease and polymerase were used to repair DNA fragments with overhangs. The 3' end of each DNA fragment was ligated to the single base A to ensure the complementary pairing of the DNA fragment with the linker. The tagged linker and DNA fragment were linked with a ligase. The linker was removed and the DNA fragment was evaluated by gel purification. A DNA fragment with a linker at both ends was selectively enriched by PCR, and a DNA library was generated. To evaluate library quality, Pico green was used for quantification and Agilent 2000 was used to detect the fragment size and distribution of the sample DNA. The library was diluted to 4–5 pmol/L and then sequenced using the Illumina HiSeq 2500 platform.
GEO
GSM4125311
GEO Accession
GSM4125311