NextSeq 500 sequencing; Vulnerability of drug-resistant EML4-ALK rearranged lung cancer to transcriptional inhibition
Vulnerability of drug-resistant EML4-ALK rearranged lung cancer to transcriptional inhibition
H3122 cells were purchased from ATCC. CrizR1 cells were a kind gift of Dr. Christine Lovly, Vanderbilt University RNA was isolated using TRIzol Reagent (Ambion), according to the manufacturer's protocol For small-RNA sequencing 1ug of total RNA from each sample was converted to a cDNA library with a NEBNext Kit (New England Biolabs) and size selected.
Cancer Research UK  Manchester Institute
E-MTAB-8563:CR1_r2_s
Experimental Factor: response to treatment
crizotinib resistant