GSM3384108: HighPD_Ctrl; Homo sapiens; RNA-Seq
Cells and barcoded beads were encapsulated into droplets of about 1nl in size. Cells were lysed and RNA were captured by barcoded beads in droplets. Droplets were then breaked to collect beads. Beads with captured RNA were reverse transcribed and exonuclease-treated as described (Online-Dropseq-Protocol-v.-3.1, http://mccarrolllab.com/dropseq/). cDNA libraries were amplified by aliquoting ~2000 beads into each PCR tubes, purified by using 0.6X AMpure Beads and quantified using Qubit. Equal amount of cDNA from each tube were pooled together, and sequencing libraries were prepared with Nextera XT tagmentation reactions using P5_TSO and Nextera_N7xx primer. Sequencing libraries were purified twice with 0.6x AMPure XP beads and once with 1x AMPure XP beads, quantified and sequenced (PE150) on Illumina HiSeq X10 sequencer using the custom primer Read1CustomSeqB.
GEO
GSM3384108
GEO Accession
GSM3384108