GSM2569987: treatment3; Capra hircus; RNA-Seq
Take 25 ~ 50mg sample in liquid nitrogen and ground to a powder，transfer all powder to a 2 ml RNase-free tube with some small balls，and immediately add the appropriate amount of lysates. The centrifuge tube into the tissue sample crusher for further crushing to fully lyse the cells and release the RNA. A total amount of 3 μg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina®(NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample.
GEO
GSM2569987
GEO Accession
GSM2569987