GSM2828321: 14_H_FN5; Nasonia vitripennis; RNA-Seq
RNA was isolated from 12 pooled heads using the TRIzol Plus RNA Purification Kit in conjunction with the PureLink RNA Mini Kit (Life Technologies, Paisley, UK) according to the manufacturer's instructions. Additional steps for “On-Column PureLink DNase Treatment During RNA Purification” were followed. mRNA library preparation for paired-end sequencing was carried out using the Illumina TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA) following the Illumina TruSeq Sample Preparation v2 (Low Sample) protocol. Briefly, mRNA was purified from total RNA samples using oligo-dT attached magnetic beads and fragmented using divalent cations at 94 °C. First-strand cDNA synthesis was carried out using reverse transcriptase and random hexamer primers. Second-strand synthesis was carried out using RNA polymerase I and RNase H. Overhangs resulting from fragmentation were converted to blunt ends and 3' ends were subsequently adenylated. Sanger indexing adapters were ligated to the fragments that were then purified and PCR-amplified to create the final cDNA libraries for sequencing.
GEO
GSM2828321
GEO Accession
GSM2828321