GSM4495071: MUT_IL6_2h_1; Homo sapiens; RNA-Seq
Following in vitro cytokine stimulation, cells were harvested by lysing the pellet in Qiagen Buffer RLT with 10μL β-mercaptoethanol/1 mL RLT. RNA was then immediately extracted from cell lysates using Qiagen RNeasy Columns according to the manufacturer's protocol. Note that the optional on-column DNase digestion was performed. RNA integrity was subsequently analyzed using the Bioanalyzer 2100 Eukaryote Total RNA Nano chip. All samples had RIN = 10. Samples were normalized to 1ug total RNA and the mRNA fraction was isolated by polyA selection. Libraries were prepared using the TruSeq Stranded mRNA Kit (Illumina).
GEO
GSM4495071
GEO Accession
GSM4495071