Targeted Bacterial 16S sequences of Magnolia grandiflora phyllosphere microbiome
Microbial community removed from plant leaf by brushing in sterile 1 mM sodium bicarbonate buffer and centrifuged. DNA extracted with MoBio Powersoil kit from pellet. Amplification of bacterial 16S rRNA gene with 8-bit indexed primers on 96-well plates (see Kozich et al. 2013. Appl Environ Microbiol 79(17): 5112-5120.). The PCR product was normalized to 25 nanograms/L per sample with the SequalPrep Normalization Plate Kit (Life Technologies).
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