Skud-RNA-rep2-TSS
Total RNA was prepared by bead-beating cells with acid phenol / chloroform and precipitation. PolyA RNA was selected using oligo-dT beads. RNA was fragmented and a 5' phosphates were removed with CIP, then 5' caps removed with TAP. 5' and 3' adapters were ligated to remaining RNA and library was PCR-amplified. For more see Arribere et al., Genome Research 2013).
SUB2779122
Sk-TSS-rep2