Targeted DNA seq of homo sapiens: FFPE Lung adeno with EGFR translocation
DNA was fragmented using sonication (Covaris Inc., Woburn, MA) to 250 bp and further purified using Agencourt AMPure XP beads. A total of 50 ng of size-selected DNA was then ligated to specific adaptors during library preparation (Illumina TruSeq, Illumina Inc., San Diego, CA). Each library was made with sample-specific barcodes quantified using quantitative PCR (Kapa Biosystems, Inc., Woburn, MA), and libraries were pooled to a total of 500 ng for capture enrichment using the Agilent SureSelect hybrid capture kit. 24 libraries were pooled and sequenced on two flow cell lanes (Illumina Inc., San Diego, CA) for an equivalent of 12 samples per lane.
Dana-Farber Cancer Institute
sample22_replicate14
alignment_software
bwa 0.5.9