Single molecule sequencing of cell-free DNA for highly sensitive detection of trisomy 21
Extracted cell-free DNA from T21 patient and normal person, and mixed in different proportions. The library preparation protocol consists of a 3’ polyA tailing step, followed by a 3’ dideoxy-blocking step. Then sequencing with the Helicos Genetic Analysis System and data analysis.
direcgenomics
Directgenomics experiment CF11