SMRT sequencing of full-length transcriptome of wild silkworm
samples of different development stages were collected.the samples were divided into two groups to prepared, the first mixed pool includes the eggs, ant worm, individuals of larval and wandering-stage, represent as F072. The second mixed pool includes the individuals of molting and moth stage, represent as F073. F072 and F073 were sequenced separately. Total RNA was extracted from each sample using the RNeasy Plus Mini Kit (Qiagen, Valencia, CA, USA) following the manufacturers protocal. SMRT sequencing was performed using the Pacific Biosciences real-time sequencer using C2 sequencing reagents.
SUB4498809
Silkworm_F073-02-T06