GSM2666628: PreS4 [RRBS]; Homo sapiens; Bisulfite-Seq
Allprep® DNA/RNA/miRNA Universal Kit (Qiagen) DNA samples were incubated with MpsI enzyme (NEB #R0106L) to fragment DNA at CCGG position to enrich for CpG regions. Bisulfite conversion was performed using the EZ DNA methylation Kit (Zymo Research) according to the manufacturer’s instructions. The DNA was then PCR amplified and ligated to TruSeq (Illumina) sequencing adapters.
GEO
GSM2666628
GEO Accession
GSM2666628