Illumina HiSeq 2500 sequencing; 10x single cell RNA Sequencing
unspecified
subs.team-2
HCA_2019-03-23_sequencingExperiment_2e4c2abd-c2e2-4464-8722-d8d1d8106f00@USI-subs.team-2
Process - Process Core - Process Id
tech_rep_1
Library Preparation Protocol - Protocol Core - Protocol Id
10x_3'_library_preparation
Library Preparation Protocol - Protocol Core - Protocol Name
10x 3' single cell library preparation
Library Preparation Protocol - Protocol Core - Protocol Description
10x Chromium single cell 3' v2 library preparation
Library Preparation Protocol - Protocol Core - Document
CG00052_SingleCell3_ReagentKitv2UserGuide_RevE.pdf
Library Preparation Protocol - Nucleic Acid Source
single cell
Library Preparation Protocol - Input Nucleic Acid Molecule
polyA RNA
Library Preparation Protocol - Library Construction Approach
Chromium 3' Single Cell v2
Library Preparation Protocol - End Bias
3 prime tag
Library Preparation Protocol - Primer
poly-dT
Library Preparation Protocol - Strand
unstranded
Library Preparation Protocol - Cell Barcode - Barcode Read
Read 1
Library Preparation Protocol - Cell Barcode - Barcode Offset
0
Library Preparation Protocol - Cell Barcode - Barcode Length
16
Library Preparation Protocol - Umi Barcode - Barcode Read
Read 1
Library Preparation Protocol - Umi Barcode - Barcode Offset
15
Library Preparation Protocol - Umi Barcode - Barcode Length
10
Library Preparation Protocol - Library Construction Kit - Retail Name
10X Chromium Single Cell 3' Solution v2 Chemistry
Library Preparation Protocol - Library Construction Kit - Manufacturer
10X Genomics
Sequencing Protocol - Protocol Core - Protocol Id
10x_scRNASeq
Sequencing Protocol - Protocol Core - Document
CG00052_SingleCell3_ReagentKitv2UserGuide_RevE.pdf
Sequencing Protocol - Instrument Manufacturer Model
Illumina HiSeq 2500
Sequencing Protocol - Paired End
true
Sequencing Protocol - Sequencing Approach
tag based single cell RNA sequencing
Input Biomaterial - Biomaterial Core - Biomaterial Id
HPSI_organoids_pooled_1
Input Biomaterial - Biomaterial Core - Biomaterial Name
pooled cells from 4 dissociated organoids
Input Biomaterial - Biomaterial Core - Biomaterial Description
pooled cells from 4 dissociated organoids (wibj_2, kucg_2, hoik_1, sojd_3)
Input Biomaterial - Biomaterial Core - Ncbi Taxon Id - 0
9606
Input Biomaterial - Genus Species - 0
Homo sapiens
Input Biomaterial - Selected Cell Type - 0
neural cell
Input Biomaterial - Total Estimated Cells
6316
HCA Process UUID
2e4c2abd-c2e2-4464-8722-d8d1d8106f00
HCA Library Preparation Protocol UUID
65ce5281-b07e-4b21-be8d-66ac5dea4d69
HCA Sequencing Protocol UUID
1547584e-d9e4-4519-a72a-91db0d0fec3c
HCA Input Biomaterial UUID
f0286bc6-33a2-4cf1-be30-b85aec3262e8
nominal_length
0
nominal_sdev
0