WGS influenza A virus: bronchoalveolar fluid immunized SCID mice
each RT-PCR sample was sheared with an M220 focused-ultrasonicator (Covaris) set to obtain peak fragment lengths of 300?400 bp. Illumina MiSeq-compatible barcode adapters were added to 100 ng of repaired end fragmented DNA. 2250 bp Illumina MiSeq paired-end sequencing was performed.
SUB6512898
EXP2_MAb37_mouse 2_dpi 39