Deep terrestrial biosphere observatory: Shield brines, microbial mats, and Banded Iron Formations at the Soudan Iron Mine- Borehole 932W2
Water samples were collected and filtered with a 0.2 micron polycarbonate filter from six boreholes that were actively discharging water.  DNA was prepped and sequencing at the MBL sequencing facility using previously described methods. Primers amplified the V4-6 region of the 16S rRNA gene. See vamps.mbl.edu for furhter information of sequencing and preprocessing.
University of Michigan
CoDL Borehole Sequencing