GSM2667717: at-endosperm-BS_replicate1_ler_wt_x_col_wt; Arabidopsis thaliana; Bisulfite-Seq
Genomic DNA of hand-dissected endosperm  from 18-28 siliques was purified using MagJET Plant Genomic DNA kit (Thermo Fisher Scientific) and sonicated in Bioruptor (Diagenode) for 30 cycles (30 sec ON, 30 sec OFF at high power) using a Bioruptor (Diagenode). The bisulfite conversion was done using the EpiTect Fast DNA Bisulfite Kit (Qiagen) during the library preparation. Bisulfite libraries were generated using Ovation Ultralow Methyl-Seq Library Systems (NuGEN) following the manufacturer's protocol using 20 ng of starting DNA.
GEO
GSM2667717
GEO Accession
GSM2667717