GSM3008517: SpleenTRNurr77Med_rep1_1122; Mus musculus; RNA-Seq
Spleen were mechanically dissociated to single cell suspensions. Tregs were sorted by flow cytometry as DAPI- TCRb+ CD4+ FoxP3-Thy1.1+ cells in 3 different bins of Nr4a1-GFP expression (low=0-20%, med=40-60%, high=80-100%). Single cells were then encapsulated in nanodroplets according to the InDrops protocol. mRNA was reverse transcribed using adapters containing oligo dT in each droplet. The emulsion was then broken and linear amplification was performed by in vitro transcription. Illumina adapters were added by reverse transcription and PCR. Library were sequenced using a NextSeq500.
GEO
GSM3008517
GEO Accession
GSM3008517