cDNA library of hemiscorpius lepturus venom gland
The mRNA isolated from venom gland of Iranian dangerous scorpion. The mRNA libraries were prepared in accordance to the Illumina TruSeq RNA sample preparation guide for Illumina Paired-End Multiplexed Sequencing.  According to the Illumina mRNA libraries preparation protocol, poly-A mRNA in the tRNA samples were first purified using   Illumina poly-T oligo-attached magnetic beads and two rounds of purification. During the second elution of the poly-A RNA,   the mRNA were also fragmented and primed with random hexamers for cDNA synthesis.  Cleaved  mRNAs  were  reverse  transcribed  into  first  strand  cDNA  using  reverse  transcriptase  and  random  primers.  The  RNA   template was then removed and a replacement strand synthesized to generate double-stranded cDNA.    ollowing the standard protocol, after the first and second strands cDNA synthesis, ends were repaired, dA base added, and   Illumina  Indexing  adapters  were  ligated.  Finally,  cDNA  fragments  that  have  adapter  molecules  on  both  ends  underwent  15 cycles  of  PCR  to  amplify  the  amount  of  prepared  material.  The  following  Adapters  sequences  were  used  for  the  libraries   preparation and amplification.
Pasteur Institute of Iran
hemiscorpius lepturus
authors
Fatemeh Kazemi-Lomedasht, Kamran Pooshang Bagheri, Mahdi Behdani, Delavar Shahbazzadeh