GSM1399618: wt_yesser_total; Escherichia coli BW25113; RNA-Seq
Rapid filtration followed by flash freezing of intact cells, cell lysis by glass bead beating, Micrococcal nuclease digestion of polysomes, purification of monosomes/disomes by sucrose-gradient centrifugation. Total mRNA was purified from the cell lysate by phenol-chloroform method, rRNA-subtracted (MicrobeExpress, Ambion) and heat fragmented for use in  sequencing library preparation. A modified small RNA protocol (Subramaniam et al., in preparation) was used for both the construction of both ribosome-protected and total mRNA sequencing libraries.
GEO
GSM1399618
GEO Accession
GSM1399618