GSM3537529: 1005_p28_R; Mus musculus; RNA-Seq
Directly after sacrifice, mice bones were embedded in OCT and snap-frozen in a hexane bath pre-cooled with dry ice. Sections of 10 microns were prepared on slides and stored at -80C. The sections were stained for visualization. LCM laser capture was used to select cells from the resting, the hypertrophic and the proliferative zones. Cells are directly picked after dissociation and deposited into lysis buffer. No RNA extraction is conducted. The cell lysis is proceed directly to library preparation. Libraries for RNA-seq were prepared by using SMART-seq2 combined with Tn5 tagmentation
GEO
GSM3537529
GEO Accession
GSM3537529