GSM4497052: G523_ScrB; Homo sapiens; ATAC-seq
Cells were harvested using Accutase and PBS 50,000 cells were harvested fresh from culture from two biological replicates, lysed on ice, spun at 4 C, and treated with Tn5 transposase (Illumina) for 30 minutes at 37 C. Libraries were amplified using the NebNext HiFi polymerase mastermix (New England Biolabs) and standard Illumina Nextera primers, and DNA was purified using the MinElute kit (Qiagen). Sequencing was performed on a NextSeq 500 using 150 cycles of paired end 75 bp sequencing
GEO
GSM4497052
GEO Accession
GSM4497052