GSM4672195: WT_IgG; Mus musculus; ChIP-Seq
Cells pellets were suspended in buffer (0.1% SDS, 1% Triton, 1 mM EDTA, 16.7 mM Tris pH 8.1, 167 mM NaCl) and were sonicated for 14 cycles  (30s on/off) using Diagenode Bioruptor Pico. 1 ug of chromatin samples were used in each IP with 1.5 ug of antibody against Myb or rabbit normal IgG, and 20 ul of Protein A beads (NEB, S1425S). The washed beads were tagged with 2ul of TDE1 from Illumina (FC-121-1030) in a 30 ul reaction. The beads were reversed cross-linked overnight at 65 degree. The DNA was amplified for 12 cycles with Illumina primers. Libraries were sequenced single-end in an Illumina Nextseq 500
GEO
GSM4672195
GEO Accession
GSM4672195