Development of microsatellite markers for a Chinese endemic plant, Torreya yunnanensis (Taxaceae)
Firstly, DNA was fragmented into about 200 to 300bp pieces using Biorupter sonicator. After fixing the cohesive ends, add the phosphate group to 5 'and add A to the 3' end. Add the linker sequence with index to DNA fragments at both ends through "TA". Finally, PCR amplification to obtain a DNA library.
SUB3351180
Ty1