Folsomia candida, field population – replicate 4
Springtails were sampled from a field location in Zaandam, the Netherlands, and transferred to standardized culturing conditions in the lab. After four months, DNA was isolated from four replicate samples, each consisting of ten animals, and used for targeted PCR. A nested approach was used to suppress amplification of Wolbachia DNA: first, a PCR was performed with primers 8f and 1525R, and the resulting PCR products were amplified with primers 357F and 518R, with appropriate barcodes and Illumina linkers.
VU University Amsterdam
Field 4