High-throughput sequencing of pit mud
Use the Magen HiPure Soil DNA Kit to refer to the step-by-step instructions to extract DNA from the pit mud sample, and use the Qubit dsDNA HS Assay Kit to detect the DNA concentration. The upstream primer 5'-CCTACGGRRBGCASCAGKVRVGAAT-3' and downstream primer 5'-GGACTACNVGGGTWTCTAATCC-3' were used to amplify the V3 and V4 regions of the 16S rRNA gene of prokaryotes.  Entrust qualified PCR products to Jinweizhi Biotechnology Co., Ltd. (Suzhou, China) for Illumina MiSeq sequencing library construction and double-end sequencing
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