GSM4618965: ID7_VisitA_Input_Control; Homo sapiens; MeDIP-Seq
Plasma was separated from whole blood and cfDNA was extracted using the Qiagen circulating nucleic acids kit. Libraries were prepared using a published protocol (PMID: 30429608). Briefly, end-repair, A-tailing and Illumina adapter ligation was performed using the KAPA hyper prep kit. Methylated cfDNA was immunoprecipated and purified using the Diagenode MagMeDIP and IPure kits. Prior to MeDIP, 10% of the adapter-ligated cfDNA sample was set aside as an input control. Both MeDIP and input control samples were then amplified using KAPA hyper prep kit protocols, followed by size selection.
GEO
GSM4618965
GEO Accession
GSM4618965