GSM2907053: KO_young_LSKs_WGBS; Mus musculus; Bisulfite-Seq
BM LSKs were sorted by BD FACSAria™ III. Total RNA of young and old WT and KO LSKs were harvested using Trizol reagent. Genomic DNA of young WT and KO LSKs were harvested using QIAamp DNA Mini Kit (Cat:51304, Qiagen). VAHTS Total RNA-Seq (H/M/R) Library PrepKit (Cat:NR603-02, Vazyme) was used with 100ng of total RNA for the construction of sequencing libraries. Accel-NGS® Methyl-Seq DNA Library Kit was used with 100ng of genomic DNA for sequencing libraries.
GEO
GSM2907053
GEO Accession
GSM2907053