GSM4332254: myeloid cells, P2FCKO_1; Mus musculus; RNA-Seq
Total RNA was extracted with Trizol and miRNeasy Mini Kit (Qiagen #217004). RNA library for sequencing was constructed from 4 μg of each DNase-treated RNA sample by using the TruSeq Stranded Total RNA LT Sample Prep Kit from Illumina (Illumina #RS-122). RNA was purified and fragmented before strand-specific cDNA synthesis. cDNA was then A-tailed, ligated with indexed adapters, PCR amplified and purified with Ampure XP beads. Library quality was validated on the Agilent 2100 Bioanalyzer.
GEO
GSM4332254
GEO Accession
GSM4332254