DNA sequences of Helianthus annuus cultivars: SAM227
For each of the 80 resequenced lines, total DNA was extracted from a bulked tissue sample (four individuals each) using the CTAB extraction protocol (Doyle 1987). Total DNA was quantified using Picogreen (Applied Biosystems/Life Technologies, Carlsbad CA, USA), and the quality was inspected usng a ND-1000 spectrophotometer (Nanodrop, Wilmington, DE, USA). Individually indexed sequencing libraries for the Illumina platform (Illumina, San Diego CA, USA) were constructed using the Illumina TruSeq DNA LT Sample Prep Kit using 3ug DNA starting material. Illumina sequencing was carried out as one library per lane on the Illumina HiSeq 2000 at MPS Sequencing Services (Montreal QC, Canada) at the ARC Biotechnology Platform (Pretoria, South Africa) as paired-ends (1*100bp). The TruSeq DNA LT library preps were done by Genome Quebec in 2011-2012 when used barcodes each one with the 6bp TruSeq Illumina barcodes 1-12.
SUB6546989
SAM227_L1