16S Round Goby Gut
Samples stored in DNA storage buffer, followed by DNA extraction with EZNA Kit. DNA was amplified via PCR for V6-V8 region of 16S rRNA gene (primers: B969F and BA1406R). Samples were cleaned with Agencourt AMPure XP PCR purification kit. Illumina kit used was 2x300bp v3 chemistry with 10% PhiX spike.
SUB5357488
RG_027_505/707