GSM4062118: BB120-minus-IPTG-3; Vibrio campbellii; RNA-Seq
RNA was extracted using Trizol and chloroform Total RNA was first treated with RiboMinus Transcriptome Isolation Kit (Bacteria) (ThermoFisher Scientific, cat#K155004) to remove excess ribosomal RNA All libraries were prepared using ScriptSeq RNA-Seq Library Preparation kit (Illumina, cat#SSV21124) according to manufacturer's protocol For all samples, sequencing barcodes selected from TruSeq Dual Index Sequencing Primer Box, Paired-End (Illumina cat#PE-121-1003) were added to the libraries during construction for multiplexing. The barcoded libraries were cleaned with AMPure XP beads (Beckman Coulter, cat#A63882), verified using Qubit4 fluorometer (ThermoFisher Scientific) and 2200 TapeStation bioanalyzer (Agilent Technologies), then pooled Pooled libraries were sequenced on Illumina NextSeq 500 (Illumina) with NextSeq75 High Output v2 kit (Illumina cat#FC-404-2005) The Libraries were sequenced on paired-end 2x38 bp run
GEO
GSM4062118
GEO Accession
GSM4062118