Tn-Seq sample from Bacillus subtilis 168 harvested 24hrs post-starvation (T24)
Tn-seq sample of Bacillus subtilis 24 hours post-starvation (T24). A magellan6 transposon library was grown in Difco Sporulation Medium to the onset of starvation, then allowed to sporulate for 24 hours. A sample was harvested, heated at 80C for 20min and germinated on LB agar. The germinated colonies were pooled and genomic DNA extracted.  The library was constructed by MmeI digestion of genomic DNA and adapter ligation, followed by a 16 cycle PCR amplification to select for transposon insertion sites. This sample was sequenced on the Illumina HiSeq 2500 platform.
Harvard University
T24