RNA-seq of Apalone ferox: subadult male blood
Libraries from total RNA of two males and two females of A. ferox were constructed by GeneCore (EMBL, Heidelberg, Germany) (mRNA-seq). The barcoded stranded mRNA-sequencing libraries were prepared using the Illumina TruSeq mRNA v2 sample preparation kit (Illumina, San Diego, CA, USA) with poly-A mRNA enrichment, implemented in the liquid handling robot Beckman FXP2. 84 bp fragments were sequenced unidirectionally in the pooled libraries using the Illumina NextSeq 500 platform.
SUB7019881
APFE_M2_RNA-Seq