RNA-Seq of Mus musculus: mouse alveolar macrophage 11 days post influenza 1
Ribo-depleted RNA was isolated from total RNA of purified murine alveolar macrophages collected on post-influenza day 9 and 11. Automated library preparation was done using the Apollo 324TM System (WaferGen Biosystems, Inc., Fremont, CA, USA) to generate a library of 100-base-pair single-end reads for RNA sequencing. DNA fragments were purified with PCRClean DX Kit from Aline Biosciences (Woburn, MA, USA). Samples were pooled in equal molar amounts, and sequenced with the Illumina HiSeq 2500 System (Illumina, Inc., San Diego, CA, USA).
SUB1338070
D11-3