GSM2828150: zmddm1a/zmddm1b 18 DAP embryo BS-seq,rep2; Zea mays; Bisulfite-Seq
Genomic DNA was extracted using CTAB method. 10 ug of genomic DNA was sheared to fragments between 300 and 500 bp. The fragments were subject to end repair, dA tailing, and ligating to methylated adapters, followed by bisulfate conversion.The bisulfate converted library were then PCR amplified (13 cycle) and purified, and sequenced on Illumina Hiseq 2500 platform.
GEO
GSM2828150
GEO Accession
GSM2828150