PacBio metabarcoding of Glomeromycotina: switchgrass rhizosphere
Extracted DNA from 250 mg soil with Qiagen DNeasy PowerSoil Kit, amplified rDNA using the AMF-specific wobble primers wSSUmCf and wLSUmBr with Phusion High-Fidelity PCR Kit from New England Biolabs, purified amplicons using AMPure XP beads, ligated amplicons with universal hairpin adapters for circular sequencing, barcoded with sample-specific primers, pooled DNA based on molarity, and sequenced on one PacBio Sequel SMRT Cell with Sequel Sequencing Kit 3.0 chemistry
SUB6570384
ccs_lbc31