Prymnesium parvum single cell transcriptome (cell sorted and captured using Fluidigm C1)
Prymnesium parvum cultures were grown under phosphorus limitation condition. Single cells were manually picked from the culture or sorted and captured using Fluidigm C1. cDNA was amplified from the single cells using SMARTer ultra-low input RNA kit. Libraries were made using Nextera kit. The same was done for RNA extracted from 10mL of the same culture for comparison.
University of Southern California
Prymnesium_single_cell_Fluidigm