wspF_rep1
The RNA was fragmented to 200 - 300 bp fragments using divalent cations under elevated temperature. First and second strand cDNA were then synthesized and treated by end repair and adapter ligation. After the 12-cycle PCR enrichment, the quality of the libraries was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies). The libraries were sequenced using the Illumina HiSeq2000 platform with paired-end protocol and read lengths of 100 nt.
SUB2558174
wspF_rep1