Illumina HiSeq 2000 sequencing; RNA-seq of blood samples from bovines immunized with a multicomponent recombinant vaccine against cattle tick.
RNA-seq of blood samples from bovines immunized with a multicomponent recombinant vaccine against cattle tick.
The whole blood collection from jugular vein of calves was performed using BD Vacutainer and PAXgene Blood RNA tube (PreAnalytix, BD), according to the manufacturer's instructions. The samples were storage at -70°C until ready for use. The samples of whole peripheral blood stabilized in the PAXgene Blood RNA tubes were isolated and purified using PAXgene Blood RNA kit (PreAnalytix, BD), according to the manufacturer's instructions.  The libraries were prepared using a Illumina TruSeq RNA Sample Prep kit according to the manufacturer's instructions.
Federal University of Sao Carlos
E-MTAB-8022:626_AA_s
Experimental Factor: stimulus
none
Experimental Factor: time
49
Experimental Factor: infect
none