miRNA-Seq of Linum usitatissimum: phloem fibers at the stage of tertiary cell wall deposition
Phloem fibers at the stage of tertiary cell wall deposition (tFIB) collected from a 5 cm long stem portion starting 1 cm below snap point. Total RNA from the tFIB sample was isolated using Trizol combined with a mirVana miRNA Isolation Kit (Thermo Fisher Scientific, Lithuania) and library of small RNA were prepared with a NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs, UK) Quality control of the library was performed using an Agilent 2100 Bioanalyzer (Agilent Technologies, USA). Further size selection was performed using Agencourt AMPure XP (Beckman Coulter, USA) to select for 143149 bp fragments. Raw reads from run were filtered to remove adapter sequences, contamination, low-quality reads and any reads shorter than 15nt and longer than 30nt.
SUB5185332
Lus_small_tFIB_1