Folsomia candida, laboratory population – replicate 2
Springtails were sampled from a population kept in standardized culturing conditions for several years. Four replicate samples were collected, each consisting of ten animals, and used for DNA isolation and targeted PCR. A nested approach was used to suppress amplification of Wolbachia DNA: first, a PCR was performed with primers 8f and 1525R, and the resulting PCR products were amplified with primers 357F and 518R, with appropriate barcodes and Illumina linkers.
VU University Amsterdam
Lab 2