16S V4 seq of metagenomes: cattle fecal sample B-2-3
Stool microbial genomic DNA was extracted from cattle fecal sample, V4 region of bacteria 16S rRNA was amplified from the isolated microbial genomic DNA. The nucleotide sequence of the primer was F515: 5  -GTG CCA GCM GCC GCG GTA A-3  , R806: 5  -GGA CTA CVS GGG TAT CTA AT-3  . PCR fragments with desired size (approximately 290bp) was purified, DNA quality and concentration was checked. Each sample had 3 replications. The purified fragments of each sample were normalized and pooled. Pyrosequencing of the samples was carried out at Bioinformatics Research Center of Macrogen (Macrogen Inc., Shenzhen, China) using Illumina Miseq system.
SUB3395102
B-2-3