GSM3685140: DNA_2N+_23_S71; Mus musculus; OTHER
Adherent cells were trypsinzed, counted and resuspended in NST-DAPI buffer. Cells were sorted on flow cytometer based on the DNA content to obtain single nuclei for scWGS Single cells isolated into 10x Lysis and Fragmentation Buffer were processed for genomic DNA amplification using SeqXE kit from Sigma Aldrich. Manufacturer's instructions very followed for gDNA amplification. Library preparation was done by end repair, adenylation, adapter ligation and PCR steps.
GEO
GSM3685140
GEO Accession
GSM3685140