GSM1939190: CON-04W-ACC-NON-MC-2; Mus musculus; MeDIP-Seq
The tissue were fixed and the nuclei were isolated and stained with NeuN antibody to be FACS sorted into neuronal and non-neuronal populations. Genomic DNA was extracted and MeDIP-seq was performed. After DNA shearing, Illumina-compatible adapters were ligated (NEB E6240) and an IP using 5mC antibody (Eurogentech BI-MECY-0100) was conducted. The IPed DNA was amplified by PCR and sequenced on Illumina Hiseq 2000.
GEO
GSM1939190
GEO Accession
GSM1939190