ChIP Elf5 in Mus musculus: f TSC
DNA from 4 IPs was pooled for each library generated with the NEB Next DNA Library Prep Master Mix (NEB E6040) according to manufacturer’s instructions. Libraries were amplified using 18 PCR cycles, purified using Agencourt AMPure XP SPRI beads (Beckman Coulter A63881) and size-selected on an agarose gel. DNA was extracted using the QiaQuick gel extraction kit (Qiagen) and its concentration determined using the KAPA Illumina SYBR Universal Lid Q Kit (KAPA Biosystems KK4824) and Bioanalyzer 2100 system (Agilent).
SUB1183113
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