GSM3986317: Sample 6 with SDC-4 siRNA silencing and no TGF-β stimulation [SD6_S69]; Homo sapiens; RNA-Seq
Invaded 3D spheroids were lysed using TRIzol reagent (Ambion® Thermo Fisher Scientific, Waltham, USA) and subsequent RNA extraction was performed using Ethanol precipitation method. cDNA library was constructed using well established Smart-seq2 protocol (Picelli et al. 2013, Picelli et al. 2014), tagmentation step was accomplished using Nextera XT kit (Illumina) and sequencing was done in Nextseq 550® at the bioinformatics expression analysis facility at Karolinska University hospital, Sweden.
GEO
GSM3986317