GSM1701100: NoDice(4-25) expressing N-terminally trunctated human Dicer N1 infected with Polio Virus Sabin 1; Homo sapiens; Human poliovirus 1 strain Sabin; ncRNA-Seq
Total RNA was isolated using TRIzol and small RNAs (<200 nt) purified using the mirVana Kit (Ambion) All small RNA input was processed with the TruSeq Small RNA Sample Preparation Kit (Illumina). Adapter-ligated RNA was reverse transcribed using SuperScript III (Life Technologies) and amplified using GoTaq green PCR master mix (Promega) with TruSeq 3′ indices.  DNA bands corresponding to ~145 bp were isolated by gel electrophoresis on 10% polyacrylamide gels.
GEO
GSM1701100
GEO Accession
GSM1701100