Illumina HiSeq 2000 sequencing; ChIP-seq data of Jun target genes and the control in zebrafish embryos
ChIP-seq data of Jun target genes and the control in zebrafish embryos
The ChIP assay was carried out within the tgf-β mutant and sibling embryos at 32 hpf in zebrafish.  The sonicated DNA was precipitated by Jun or Normal Rabbit IgG antibodies. Afterward, the precipitated DNA was collected by QIAquick PCR Purification Kit. The gene binding profiles were detected by deep-sequencing.  DNA samples were end-repaired, A tailed, and adaptor ligated using TruSeq Nano DNA Sample Prep Kit (#FC-121-4002, Illumina), following the manufacturer’s instructions. 200 – 500 bp fragments were selected at the gel size fractionation step. The final size of the library was confirmed by gel electrophoresis.
Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
E-MTAB-5699:Si_input_1_s
Experimental Factor: genotype
wild type
Experimental Factor: immunoprecipitate
IgG