GSM2667794: npc_rna_rep2; Homo sapiens; OTHER
Libraries were prepared using a slightly modified version of the ribosome profiling protocol (Ingolia 2012). Protected footprints were generated by RNase digestion; fragmented RNA was generated using limited alkaline hydrolysis. Polysomal RNA was extracted from sucrose gradient fractions by ethanol precipitation. RNA was then DNase treated, subjected to acid phenol:chloroform extraction, and ethanol precipitated. Cytoplasmic and nuclear RNA was extracted using TRIzol extraction (Life Technologies) and ethanol precipitation. Total RNA integrity was verified using a BioAnalyzer (Agilent).
GEO
GSM2667794
GEO Accession
GSM2667794