GSM2402812: NT_r2; Homo sapiens; RNA-Seq
Total RNA was collected using the Rneasy Mini Kit (Qiagen) according to the manufacture's instructions. Indexed PolyA libraries were prepared using  200ng of Total RNA and 14 cycles of amplification in the Agilent Sure Select Strand Specific RNA Library Prep Kit for Illumina Sequencing  (Agilent  Cat No: G9691A). Libraries were quantified by qPCR using a Kapa Library Quantification Kit for Illumina sequencing platforms  (Kapa Biosystems Inc. Cat No: KK4835). Paired-end  75bp sequencing was carried out by clustering  2pM of the equimolar pooled libraries on a NextSeq 500 sequencer  (Illumina inc.)
GEO
GSM2402812
GEO Accession
GSM2402812