MeDIP-Seq of Chrysemys picta - hatchling gonads - male replicate 1
We extracted DNA from the gonads of 3 month-old C. picta hatchlings (10 males and 10 females, and pooled in groups of 5 hatchlings for replication) using the Gentra Puregene DNA extraction kit (Gentra) following the manufacturerÍs instructions. DNA was processed by BGI Americas following the MeDIP protocol where DNA was fragmented, denatured , and the methylated DNA was immunoprecipitated using the MagMEDI kit (Diagenode). A positive control (methylated DNA) and a negative control (unmethylated DNA) were used at the MeDIP step to ensure that the MeDIP worked properly. The methylated DNA was size selected (100-300bp) and sequenced using the Illumina HiSeq paired-end protocol.
SUB2559666
M1