RNAseq (Tn5Prime) of individual human B cells sorted for CD27+ and CD38+
The Tn5Prime library preparation method used to analyze these cells is a modification of the Smartseq2 method. It uses a template switch oligo that incorporates a Illumina adapter sequence and a cellular index into cDNA. The cDNA of many cells can then be pooled for PCR. The other Illumina adapter is added by the Tn5 enzyme  loaded only with that one adapter. In this way, Read 1 is anchored to the 5' end of transcripts while Read 2 is distributed randomly throughout the transcripts.
SUB3152867
SHC1_CD27_CD38_Plate1_A3_Cell7_Tn5Prime