Effects of ocean acidification on gene expression of juvenile rockfish (Sebastes spp.)
Juvenile rockfish (Sebastes caurinus and S. mystinus) were exposed to four pCO2 treatments (uatm) for 3-4 months (400-control, 700, 1200, 2000). 2-4 fish were sacrificed per treatment and samples prepared for Illumina RNAseq (HiSeq 2000). Fifteen S. caurinus samples were multiplexed into 2 lanes (7 samples into a paired end 100 bp, 8 samples into a single end 100bp). Twelve S. mystinus individuals were multiplexed into one paired end 100 bp lane.  SPECIFIC DETAILS: Muscle tissue was dissected and removed, flash frozen in liquid nitrogen, and stored at -80C.  Total RNA was extracted using the Qiagen RNeasy kit (Cat#74104). mRNA was extracted from 1 ug of total RNA using the NEBnext poly(A) mRNA magnetic isolation module Kit (Cat#E7490S) for the construction of sequencing libraries. Libraries were prepared according to the NEBnext Ultra Directional RNA library kit instructions accompanying the NEBnext Ultra Directional RNA library kit (Cat# E7420G).
California State University, Monterey Bay
Sample 6-pCO2 1200 uatm