Illumina HiSeq 2000 sequencing; Transcriptome analysis of miRNA expression in skeletal muscle of Yorkshire pigs with  differential residual feed intake
Transcriptome analysis of miRNA expression in skeletal muscle of Yorkshire pigs with  differential residual feed intake
The castrated purebred Yorkshire boars from Hubei Jinlin Pedigree Seed Husbandry Ltd. were individually fed a commercial feedlot ration using ACEMA 64 automated individual feeding systems in Agricultural Ministry Breeding Swine Quality Supervision Inspecting and Testing Center (Wuhan). The recording of daily feed intake (DFI) were began when the pigs body weight reached 35 Kg and ended when the body weight reached 90 Kg. Every time, pigs were weighed after 24 hours of fasting. The average feed intake (ADG) were got by the total weight gain and the number of days on feed. Total RNA was extracted with Trizol reagent (Invitrogen, USA), according to the product manufacturer's instructions. RNA sequencing libraries were prepared for each RNA-seq sample using TruSeq Stranded Total RNA Sample Preparation kit (Illumina) and all of the procedures and standards were performed according to the manual supplied with this kit.
KEY LAB OF AGRICULTURAL ANIMAL GENETICS AND BREEDING, MINISTRY OF EDUCATION, COLLEGE OF ANIMAL SCIENCE AND VETERINARY MEDICINE, HUAZHONG AGRICULTURAL UNIVERSITY,
E-MTAB-2949:RFI_L
Key Lab of Agricultural Animal Genetics and Breeding, Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University,
E-MTAB-2949:RFI_L
Experimental Factor: behavior
low residual feed intake
LIBRARY_STRAND
first strand