Infantile heart 1: RNA-seq human cardiac myocyte nuclei
Nuclear RNA was extracted from sorted cardiac myocyte nuclei using either the RNeasy Micro Kit or the AllPrep DNA/RNA Micro Kit (Qiagen) including on-column DNAse digestion. RNA was reverse transcribed and amplified with the Nugen Ovation RNA-seq System V2 (Nugen). The resulting cDNA was fragmented (Bioruptor, Diagenode). Sequencing libraries were constructed from 100 ng fragmented cDNA using the NEBNext Ultra DNA Library Prep Kit (NEB). The necessary PCR cycles were determined by qPCR after addition of Eva Green (Biotium).
SUB3380142
I1_nuclear_RNA_seq