GSM4064426: CK5-trace luminal [MM009]; Mus musculus; RNA-Seq
For RNA sequencing of lineage-marked cells, YFP-positive cells were directly sorted into lysis buffer. cDNA was synthesized using SMART-Seq v4 Ultra Low Input RNA Kit (Clontech) with 12-16 cycles of PCR amplification, depending on the number of sorted cells. Amplified cDNA was verified using the Agilent 2100 Bioanalyzer. For RNA isolation from bulk wild-type prostates, 2-3 prostates from three-week males (n=6 pools) were collected using the MagMax96 for Microarrays Total RNA Isolation kit (Invitrogen). Libraries were prepared by the Columbia Genome Center. Poly-A pull-down was used to enrich mRNAs from total RNA samples (200ng-1ug per sample, RIN>8 required). Libraries were prepared using the Illumina TruSeq RNA prep kit.
GEO
GSM4064426
GEO Accession
GSM4064426