Illumina MiniSeq paired end sequencing; DNA-seq or genomic RNA region of HIV-1 reverse transcriptase, protease and integrase gene
DNA-seq or genomic RNA region of HIV-1 reverse transcriptase, protease and integrase gene
All samples are collected at University Hospital for Infectious Diseases in Zagreb as a part of the study of molecular epidemiology of HIV infection. For this purpose clinical plasma samples were collected from HIV-1 patients. HIV-1 RNA was extracted from 500 μl of patient's plasma sample by using the QIAmp Viral RNA Mini Kit (Qiagen, Hilden, Germany). HIV-1 RNA was extracted with standard procedure and reversly transcribed with SuperScript® III First-Strand Synthesis System for RT-PCR (Invitrogen, Carlsbad, CA) and HIV-1 specific primer.
University of Zagreb, School of Medicine
E-MTAB-8153:HIV-1_NGS37_p
Experimental Factor: clinical information
HIV isolated from blood plasma of a 37-year-old patient