MinION sequencing
A sequencing library was constructed using the Ligation Sequencing Kit 1D, SQK-LSK108 (Oxford Nanopore Technologies) starting with 2ug of restricted integrity gDNA without a fragmentation step. The DNA was repaired using the NEBNext FFPE Repair Mix (New England Biolabs), end-repaired and adenylated with the NEBNext Ultra II End Repair and A-Tailing Module (New England Biolabs) and MinION AMX adapters (Oxford Nanopore Technologies) were ligated using the NEB Blunt/TA Ligase Master Mix (New England Biolabs). Each step was followed by purification with AMPure XP Beads. The DNA/beads ratio was 1 (V/V) after the end-repair and adenylation step. After the repair and the final purification/size selection steps the DNA/beads ratio was 0.4 (V/V) in order to eliminate all fragments below 2kb.
CNAG
ena-EXPERIMENT-CNAG-25-10-2019-13:14:10:114-1
Centro Nacional de Análisis Genómico
ena-EXPERIMENT-CNAG-25-10-2019-13:14:10:114-1