5' OH RNA seq: E. coli  expressing RNaseJ-mt (Rv2752c, +IPTG) 60 min
To select for 5' OH RNA, total RNA was digested with 1 U of Terminator 5-Phosphate-Dependent Exonuclease and phosphorylated with 3 u of T4 PNK. Adaptors were ligated with T4 RNA ligase 1, followed by Reverse transcription with Superscript IV, and PCR with adaptor-specific primers. See Schifano et al. (2014) Nat. Comm. PMID 24709835.
SUB4499757
VJ80