GSM4672447: CTCF knockout biological replicate 1 ATAC-seq; Mus musculus; ATAC-seq
DNA from whole heart tissue was manually disassociated, isolated nuclei were quantified using a hemocytometer, and 100,000 nuclei were tagmented as described in Buenrostro et al. (PMID: 24097267) Libraries were prepared according to Illumina's instructions (Nextera Library Prep Kit). Tagmented DNA was then purified using the MinElute PCR purification kit (Qiagen), amplified with 10 cycles of PCR, and purified using Agencourt AMPure SPRI beads (Beckman Coulter).  Resulting material was quantified using the KAPA Library Quantification Kit for Illumina platforms (KAPA Biosystems).
GEO
GSM4672447
GEO Accession
GSM4672447