Illumina HiSeq 4000 paired end sequencing
After poly-A based mRNA enrichment with oligo-dT magnetic beads and 500ng of total RNA as the input material, the mRNA was fragmented. The strand specificity was achieved during the second strand synthesis performed in the presence of dUTP instead of dTTP. The blunt-ended double stranded cDNA was 3´adenylated and Illumina platform compatible adaptors with unique dual indexes and unique molecular identifiers (Integrated DNA Technologies) were ligated. The ligation product was enriched with 15 PCR cycles and the final library was validated on an Agilent 2100 Bioanalyzer with the DNA 7500 assay.
CNAG
ena-EXPERIMENT-CNAG-15-11-2019-11:15:33:439-1
Centro Nacional de Análisis Genómico
ena-EXPERIMENT-CNAG-15-11-2019-11:15:33:439-1