GSM2564749: 129_OPC_48hrPostT3; Mus musculus; RNA-Seq
Cells were lysed directly in 1 ml of TRIzol (Invitrogen) and stored at -80°C. Once all samples were collected, samples were thawed on ice and RNA was separated with chloroform using Phase Lock Gel tubes (5prime). RNA was isolated using the miRNeasy Mini Kit (Qiagen) according to the manufacture's protocol. One microgram of each sample was then subject to ribosome depletion, fragmented, and library prepared using the TruSeq Stranded Total RNA Kit with Ribo Zero Gold (Illumina) according to the manufacturer's protocol and indexed using TruSeq adapters. One hundred base pair paired-end reads were generated for each sample on the Illumina HISeq 2500 (Case Western Reserve University Sequencing Core; Cleveland, OH).
GEO
GSM2564749
GEO Accession
GSM2564749