Raw data of sham group-1
mRNA was isolated using magnetic beads conju-gated with oligo (dT) capture probes. First-strand cDNAwas synthesized from mRNA fragments by reverse tran-scriptase using random hexamers (Invitrogen, San Diego,CA, USA) as primers, and second-strand cDNA was thensynthesized using reaction buffer, dNTPs, RNase H andDNA polymerase I (Amersham Biosciences, Piscataway,NJ, USA). The resulting cDNA fragments were puri-fied using a QIAquick PCR Purification Kit (Qiagen) andwere then treated with T4 DNA polymerase (Illumina, SanDiego, CA, USA), T4 PNK (Illumina), and Klenow DNApolymerase (Illumina) to convert the overhangs into bluntends. The cDNAs were ligated to sequencing adapters(Illumina) and separated by agarose gel electrophoresis toselect suitable fragments for PCR amplification.
SUB5891446
B1