GSM1401414: 293 RISC-Associated Small RNA (Abcam AB57113 Immunoprecipitation); Homo sapiens; RIP-Seq
Immunoprecipitation of RISC with followed by mirVana miRNA isolation as previously described (Flores et al, 2013 [PMID 23536669]) Illumina TruSeq Small RNA Sample Preparation Kit was used to ligate adapters with small RNA purified with the mirVana miRNA Isolation Kit.  Reverse transcription was performed with Invitrogen SuperScript III and PCR amplification using Promega's GoTaq Green Master Mix
GEO
GSM1401414
GEO Accession
GSM1401414