GSM3927335: wild-type H3K4me3; Saccharomyces cerevisiae BY4741; ChIP-Seq
Lysates were clarified from MNase-treated nuclei and histone-DNA complexes were isolated with antibody. 25% (vol. at OD 0.6) S.pombe cells were added as a spike-in control. 10 uL of anti-H3K4me3 (Millipore, 05-745R) were used for precipitation Libraries were prepared using the NEBNext Ultra II DNA Library Kit (NEB) and sequenced on an Illumina Nextseq 500 platform
GEO
GSM3927335