GSM1940052: cJUN IP; Mus musculus; ChIP-Seq
ChIP was performed following a previously described protocol (Vokes et. Al, 2007). Intestines wer snap-frozen in liquid and ground in a mortar and pestle to dissociate the tissue to single cells. DNA and protein were then crosslinked and sonicated to an average 500 bp fragment size. cJUN bound regions were immunoprecipitated overnight using anti cJUN Polyclonal Rabbit antibody (Santa Cruz sc-44) bound to Protein G Dyanabeads). Input control consisted of sonicated DNA. ChIP libraries were prepared for sequencing using standard Illumina protocols
GEO
GSM1940052
GEO Accession
GSM1940052