Ab10-II-Tel mRNA, sample 3
Frozen anthers were hand ground with a plastic pestle in Buffer RF, the stabilizing extraction buffer from Qiagen RNeasy Plant Mini kit (Cat# 74903). The protocol from the kit was then followed as specified to isolate RNA. mRNA sequencing libraries were prepared using a stranded Kapa Biosystems mRNA kit, Cat# KK8420 with dual indexing.
University of Georgia
K2_6