GSM4176083: fli1a:GFP+ sox10:DsRed+ cells from 36 hpf wt embryos; Danio rerio; ATAC-seq
FACS-sorted cells were centrifuged at 700 x g, and the supernatant was carefully removed. Next, 20 μl lysis buffer (10 mM Tris-HCl pH 7.4, 5 mM MgCl2, 10% DMF, 0.2% NP40) was added to the cell pellet and mixed by pipetting up and down 5-10 times. A 30-μl aliquot of reaction buffer (10 mM Tris-HCl pH 7.4, 5 mM MgCl2, 10% DMF) containing homemade Tn5 transposase was then immediately added to the lysis, and the samples were incubated for 20 min at 37℃. DNA fragments were extracted with the Qiagen MinElute kit DNA fragments are amplified by PCR for 5 cycles using NEBnext master mix, with primers containing various indexes. Additional cycles are determined by qPCR on 1/10 of the amplified libraries. After amplification, the libraries are cleaned using AMPURE beads, to only keep the fragments larger than 100bp. The libraries are pooled before sequencing. 
GEO
GSM4176083
GEO Accession
GSM4176083