Illumina HiSeq 2500 paired end sequencing; RNA-seq analysis of MES-MM 98 and REN mesothelioma cell lines modulated for miR-24-3p expression
RNA-seq analysis of MES-MM 98 and REN mesothelioma cell lines modulated for miR-24-3p expression
Stable modulation of miR-24 expression by Lentiviral transduction. REN cell line reference paper : Smythe WR, Kaiser LR, Hwang HC, Amin KM, Pilewski JM, Eck SJ, et al. Successful adenovirus-mediated gene transfer in an in vivo model of human malignant mesothelioma. The Annals of thoracic surgery 1994;57:1395-401. MM98 cell line reference paper: Orecchia S, Schillaci F, Salvio M, Libener R, Betta PG. Aberrant E-cadherin and gamma-catenin expression in malignant mesothelioma and its diagnostic and biological relevance. Lung Cancer 2004;45 Suppl 1:S37-43 RNA extraction by Trizol reagent Random primed cDNA libraries were constructed from 100 ng of total RNA. A paired-end (2×125) run was performed on a Genome Sequencer Illumina HiSeq 2500 (GATC Biotech, Germany) with 50 million reads per sample.
INGM, National Institute of Molecular Genetics, “Romeo ed Enrica Invernizzi”, Milan, Italy IGM - CNR, Pavia, Italy
E-MTAB-7079:REN miR-Zip24 3_p
Experimental Factor: cell line
REN
Experimental Factor: genotype
miR-24-3p lentiviral knockdown