Switchgrass cultivar root and soil microbiome
Bacterial (16S primers 515f/806r) and fungal (ITS1 primers ITS1-F/ITS2) reads were assembled, and quality filtered (discarded reads with quality filter maxEE <1.0 and base pairs < 250) using Usearch (version 10.0.240). Sequences were dereplicated, clustered, chimera checked, filtered de novo, and clustered into unique operational taxonomic unites (OTUs) based on 97% identity using the default settings with Usearch UPARSE function. Representative sequences were aligned and classified using the Silva (version 123) and Unite (7.2) reference databases for bacterial and fungal sequences, respectfully (Quast et al. 2012; Nilsson et al. 2018).
SUB6413861
TMCSGendoJ5