GSM4062104: SacPom_plus_plus_2; Schizosaccharomyces pombe; OTHER
For HEK 293T cells, total RNA is extracted using phenol:chloroform method. For yeast, total RNA is extracted using the hot-acid-phenol method. Ribosome-protected footprints were purified from monosome fractions by size selection and gel extraction. Following 3' dephosphorylation these fragments were subjected to adaptor ligation, RT, circularization and PCR amplification.
GEO
GSM4062104
GEO Accession
GSM4062104