GSM4174981: P14L01HC; Rattus norvegicus; RNA-Seq
Rats were sacrificed through rapid decapitation, followed by immediate brain extraction. The whole hippocampus was rapidly dissected on ice, fast-frozen at –40°C, and stored at –80°C before processing. TRIzol reagent (Invitrogen, Calsbad, CA) was used to extract total RNA, followed by purification using RNeasy RNA purification columns (Qiagen, Valencia, CA). The quality and concentration of the RNA was determined using an Agilent bioanalyzer (Palo Alto, CA) and wavelength absorbance (260/280 nm ratio) by Nanodrop. Purified RNA was shipped to the Genomic Services Laboratory at HudsonAlpha (https://gsl.hudsonalpha.org/index) for short-read (50 bp length) single-end sequencing. RNA Qubit was used to assess concentration (range: 720-1120 ng/uL). The dilutions were denatured through heat application at 70˚C for two minutes and then checked for quality with a RNA Nano bioanalysis chip. Indexed non-stranded cDNA libraries were then prepared using standard Illumina procedures and a TruSeq library preparation kit (Illumina) after which they were pooled for sequencing with four samples per pool. The pooled libraries were clustered on a HiSeq flowcell (Illumina) and sequenced using a first generation HiSeq 2000 sequencer.
GEO
GSM4174981
GEO Accession
GSM4174981