medaka d-rR blastula RNA-seq
RNA was isolated from medaka blastula using ISOGEN (Nippon Gene) and RNeasy kit (QIAGEN). Purified RNA were treated with Ribominus eukaryote kit for RNA-seq (Life Technologies). RNA-seq library was prepared using TruSeq RNA-seq sample prep kit (Illumina). The PCR products were purified and size fractionated using a bead-mediated method (AMPure, Ambion). Sequencing was conducted on Genome Analyzer IIx platform (Illumina).
University of Tokyo
medaka d-rR blastula RNA-seq