REPLIg WTA of mosquito microbiome sequenced on Illumina MiSeq
The REPLI-g WTA product from virus-positive sample 4.1 was prepared for Illumina sequencing using Illumina's Nextera XT library prep kit (FC-131-1024), following the manufacturer's protocol through the library clean-up step.  Manual normalization was performed following DNA quantitation of the CAN product using the Qubit fluorometer to ensure a sufficient quantity of library was generated.  The library was then diluted using a conversion factor of 2 to 2nM and pooled with other libraries.  The libraries were added to a cartridge at a final loading concentration of 12pM using a MiSeq Reagent Kit V2 (MS-102-2002).   A 2 x 151 paired-end run was performed on the Illumina MiSeq system (SY-410-1003) using the FASTQ only workflow.
SUB3153241
EVG-2016_CxCdci_4_1_RepliG_Illumina_1