GSM4061849: OE-3_rep1; Oryza sativa; RNA-Seq
For gene expression profiling, we took the leaf samples at the early heading stage. 3 replications of the top second leaves for WT, the OE lines (OE-2, OE-3, OE-4) and the CRISPR lines (C-13, C-14, C-16) were harvested in the field at about 9:00 a.m. Leaf samples were frozen in liquid nitrogen immediately and then stored in -80℃ fridge before RNA extraction. For RNA extraction, we used the PureLink RNA Mini Kit (Life Technologies Corporation). We firstly grinded the leaf sample with liquid nitrogen, then followed the protocol of PureLink RNA Mini Kit. RNA libraries were prepared for sequencing based on standard Illumina protocols.
GEO
GSM4061849
GEO Accession
GSM4061849