GSM3986340: HUVEC STIFF rep5; Homo sapiens; RNA-Seq
Cells residing for 24 h on these gels were then harvested and lyzed using the QIAshredder Kit (Qiagen; 79656). mRNA was harvested using the RNeasy Plus Micro Kit (Qiagen; 74004) and eluted in 30 μL RNAase free water. RNA concentrations were measured using the nanodrop machine and were comparable between conditions. RNA quality and quantity were confirmed via bioanalyzer analysis performed by the Stanford Protein and Nucleic Acid (PAN) Facility. RNA libraries were prepared by the Stanford Functional Genomics Facility using the KAPA stranded RNA-seq kit with RiboErase (Kit code KK8483, Roche Cat. # 07962282001), for a fragment length of 200-300 bp. Sequencing was run on the Illumina NextSeq 500 System using the High-Output Kit with 2x75 read length. We had an average of 30 million reads per sample. We used 2 lanes with 12 samples each ensuring that from each condition 3 replicates go to lane A and the other 3 to lane B (total number of replicates N = 6).
GEO
GSM3986340