Comparative sequencing of lab-evolved ethanol-tolerant and parental strains of MG1655
Samples were prepared following the Illumina Genomic DNA sequencing protocol (Rev. B), using a NEBNext genomic DNA sample preparation kit (New England Biozymes). Genomic DNA was isolated using a DNeasy mini kit (Qiagen). Nebulizers were obtained from Invitrogen. Primers and adapters were obtained from IDT. Adapter sequences included three base pair barcodes to allow multiplexing.
Columbia University
Ethanol tolerant MG1655