GSM2566898: Exp 2 Treated rep 1; Mus musculus; RNA-Seq
RNA was extracted from the dissected mouse tissues using TRI-reagent (Sigma), following the manufacturer’s instructions, which included a chloroform phase separation and ethanol precipitation. RNA was resuspended in 70 µL of RNase-free water. RNA samples were assessed for quantity and quality using a NanoDrop UV spectrophotometer (Thermo Fisher Scientific Inc), conventional RNA electrophoresis and using a Bioanalyser (Agilent Technology Inc). All RNA integrity numbers (RIN) for the analysed samples ranged from 9 to 9.5 mRNA libraries were prepared at the Ramaciotti Centre for Genomics (UNSW University of New South Wales, Australia). The Illumina TruSeq Stranded Total RNA Prep Kit was used for Experiments 2 .The six RNA-Seq libraries in Experiments 2 were sequenced using the Illumina NextSeq 500. R1.fastq and R2.fastq files were produced for each sample.
GEO
GSM2566898