RNA-Seq of Scaphiopus couchii: tapole
An individual tadpole was eviscerated and snap-frozen in liquid nitrogen. Subsequently, the whole body was homogenised in Trizol reagent and Total RNA was extracted following the manufacturer's protocol (Invitrogen). The RNASeq library was prepared from total RNA using the TruSeqtranded mRNA LT Sample Prep Kit (Illumina Inc., Rev.E, October 2013) and the library was sequenced using TruSeq SBS Kit v3-HS, in paired end mode with the read length 2x76bp.
SUB4501191
Scaphiopus_couchii_24h_L_10