LL-1
Seedlings (stage with 56 leaves) of S. spontaneum were subjected to cold stress in an incubator. The growth conditions were as follows: temperature, 3C; light intensity, 250300 molm2s1; photoperiod, 12 h; and relative humidity 6070%. After three days of cold stress treatment, the leaves of the seedlings from the control and low temperature stress treatments were collected, and 3 replicates of sampling were used per treatment. The +1 leaves (top visible dewlap leaf) of the same size and phenotype was collected as the test material. Clone 12-23 under cold stress was labeled as LL, and the control was labeled as LC. Clone 15-28 under cold stress was labeled as HL, and the control was labeled as HC. Three biological replicates were used for each treatment.
SUB6549569
LL-1