Cross-Sectional Study of Diet and Stool Microbiome (COMBO)
Each amplicon library was constructed by amplifying the16S rRNA gene using the BSF8 and and the BSR357 primer pair. Primers contained DNA barcode sequences such as those described by Hamady et al. 2008, and the recommended 454 adapter sequences. Amplification conditions are described in McKenna et al 2007, with exception that the polymerase used was AccuPrime (Invitrogen, Carlsbad, CA, USA).
UPENNBL
HMP-phs252rrna_GVJ07HB01
library_strategy
targeted-locus
gene
16S rRNA V1-V2 region