Targeted Illumina Paired Sequencing of eMERGEseq Variant Gene Panel
Genomic DNA was randomly fragmented by sonication and ligated to Illumina multiplexing PE adapters. The adapter-ligated DNA is then PCR amplified using primers with sequencing barcodes (indexes). For targeted capture, the pre-capture library is enriched by hybridizing to biotin labeled in-solution probes (eMERGE-Seq).  The eMERGE-Seq custom capture design contains 109 genes and 1,551 SNVs. Baylor alignment to hg19 (build 37) pipeline includes bwa and GATK software.
UW-BIME
1000073850