Helix aspersa CNS transcriptome (TaxId: 6535)
The CNS (n = 20 ), encompassing the cerebral ganglion, visceral, pedal and pleural ganglion were surgically removed and stored in RNAlater (Invitrogen) at -80°C until required. RNA was isolated from CNS (~100 mg/species) using the TRIzol (Invitrogen) isolation procedure and concentration and quality was assessed using a Nanodrop spectrophotometer. RNA was provided to BGI (Shenzhen) for sequencing (~5 Gigabases, normalised) using the Illumina HiSeq 2000 sequencing platform.
SUB1902118
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