Bisulfite-Seq analysis of RRBS1242 derived from human Mobilized CD34 cells
Smith et al., Methods 48, 226-232
BI
RRBS1242
BISULFITE_CONVERSION_PROTOCOL
2x5hrs Epitect Kit
DNA_PREPARATION_INITIAL_DNA_QNTY
20 ng
EXTRACTION_PROTOCOL_SONICATION_CYCLES
NA
DNA_PREPARATION_ADAPTOR_LIGATION_PROTOCOL
T4 Ligase (2,000U/ul) 16C o/n
DNA_PREPARATION_ADAPTOR_SEQUENCE
5' P-GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG and 5' ACACTCTTTCCCTACACGACGCTCTTCCGATCT
LIBRARY_GENERATION_PCR_R_PRIMER_SEQUENCE
5' CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT
DNA_PREPARATION_POST-LIGATION_FRAGMENT_SIZE_SELECTION
120-370
BISULFITE_CONVERSION_PERCENT
>99%
EXTRACTION_PROTOCOL
Standard Protocol (Smith et al., Methods 48, 226-232)
LIBRARY_GENERATION_PCR_PRIMER_CONC
25uM
DNA_PREPARATION_FRAGMENT_SIZE_RANGE
30-280
EXPERIMENT_TYPE
DNA Methylation
LIBRARY_GENERATION_PCR_THERMOCYCLING_PROGRAM
Smith et al., Methods 48, 226-232
LIBRARY_GENERATION_PCR_PRODUCT_ISOLATION_PROTOCOL
Gel size selection
LIBRARY_GENERATION_PCR_F_PRIMER_SEQUENCE
5' AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
LIBRARY_GENERATION_PCR_TEMPLATE_CONC
NA
LIBRARY_GENERATION_PCR_POLYMERASE_TYPE
Pfu Turbo Cx hot start
LIBRARY_GENERATION_PCR_NUMBER_CYCLES
15
EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR
NA
GEO Accession
GSM772730