mirna-seq of mus musculus:MC38_WT_Rep3
High-throughput sequencing was performed according previous described methods. Berifly, total RNA were extracted from cells using mirVana miRNA Isolation Kit (Invitrogen Cat AM1560). Library creation and sequencing were performed by the Mayo Clinic Genome Analysis Core.  For miRNA-Seq, the sequencing libraries were prepared using 1 g of total RNA per the manufacturers instructions for the NEBNext multiplex small-RNA kit (New England Biolabs, Ipswich, MA). Sequencing was performed using one lane on an HiSeq 4000 (Illumina) instrument using 50 base pair paired end mode.
SUB7321935
MC38_WT_Rep3