GSM2829722: SKF5; Carassius auratus; RNA-Seq
Total RNA was extracted using the RNeasy Micro Kit (Qiagen) including an on-column DNase treatment to remove genomic DNA. The concentration of total RNA was determined using the Qubit RNA Assay Kit (Life Technologies). In order to evaluate the quality of the total RNA, RNA integrity number (RIN) was assessed using Agilent RNA 600 Nano Reagents and RNA Nano Chips in Agilent 2100 Bioanalyzer (Agilent Technologies). Sequencing of 10 RGC cultures was performed by MR DNA (www.mrdnalab.com) following the manufacturer's guidelines (Illumina HiSeq) for paired end sequencing (151 bp).
GEO
GSM2829722
GEO Accession
GSM2829722