GSM4219776: OPC_rep1 (RNA-seq); Mus musculus; RNA-Seq
10^6 cells were pelleted, resuspended in TRIzol, and frozen at -80 degrees Celsius. Total RNA was isolated using the Qiagen Rneasy Mini Kit (ThermoFisher #15596026), beginning with chloroform extraction and separation by PhaseLock gel tubes (5 Prime #2302830). 1ug of each sample was rRNA depleted (Ribo-Zero Gold, Illumina #MRZG12324), fragmented, and indexed using the TruSeq Stranded mRNA Library Preparation Kit (Illumina #RS-122-2103) per the manufacturer's protocol.
GEO
GSM4219776
GEO Accession
GSM4219776