Genome Seq of Coereba flaveola: adult female genomic DNA from blood
Bananaquit DNA from the two specimens was barcoded for individual identification and paired-end and mate-pair libraries were constructed with Illumina’s PCR-free prep kits.  The mate-pair libraries were gel-purified and the 2-4 kb segment was isolated.  The samples were sequenced in two lanes on an Illumina HiSeq2500, with one lane used for paired-end libraries (2×100 bp read lengths) and one lane for mate-pair libraries.  Subsequently, Trimmomatic (Bolger et al. 2014) was used to remove the Illumina adapters and clip low-quality bases (Phred < 20) from both the 5’ and 3’ ends of the reads.
SUB2094540
SK009_MatePair_R1