T98G_2
Total RNA including miRNA was extracted as described previously. Small-RNA sequencing libraries were constructed using the NEBNext Multiplex Small RNA Library Prep Set for Illumina sequencing kit (New England Biolabs). Samples were barcoded and size restricted to ~150bp an acrylamide gel and purified.  The final purified and barcoded products are then pooled in equimolar amounts for single-end 50 bp sequencing on an Illumina HiSeq2500 instrument.
SUB1183476
T98G_2