Amplicon-seq of tprKTreponema pallidum
Purified amplicons were used to prepare the DNA libraries for next-generation sequencing by using Next Ultra DNA Library Prep Kit (NEB, Ipswich, MA, USA). Briefly, the quality and concentration of DNA was determined by the Qubit Fluorometer (ThermoFischer, Carlsbad, CA, USA.). And then each DNA sample was subjected to end repair and adaptor ligation, subsequently, the library was amplified by PCR.4L of constructed DNA library was subjected to 2% agarose gel electrophoresis for size selection. Additional quality control measure like size distribution was determined by using Agilent 2100 Bioanalyzer with the DNA1000 Kit (Agilent, Santa Clara, CA, USA).
SUB5004527
S-8