RNA-Seq of Brassica oleracea infected by whitefly versus control
Leaf samples were ground in liquid nitrogen and total RNA was extracted using the RNeasy Plant Mini kit (Qiagen, Hilden, Germany) following the manufacturers protocol. Total RNA was quantified by Nanodrop 1000 and analyzed with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) to confirm its integrity before sequencing. RNA sequencing (RNA-seq) library construction and sequencing was done at the Utrecht Sequencing Facility (Utrecht, the Netherlands). Libraries were constructed using the TruSeq Stranded mRNA polyA Sample Preparation kit (Illumina, San Diego, USA) and thereafter sequenced on an Illumina NextSeq500 platform using a paired-end 150-base pair high output run.
SUB4502644
SW3