GSM4062026: DFN3_LALA_N1; Macaca mulatta; RNA-Seq
Total RNA was extracted from sorted NK cells and enriched for polyadenylated species by two sequential rounds of binding to oligo-dT dynabeads (Life Technologies), chemically fragmented in the presence of Mg2+, and reverse transcribed using Superscript III reverse transcriptase (Life Technologies). Second strand cDNA synthesis, end repair, A-tailing, and sequencing adaptor ligation were performed using NEBNext enzyme modules (New England Biolabs). Libraries were amplified using universal and indexed primers from the NEBNext system with Kapa 2x Hot Start Readymix (Kapa Biosystems). Amplified libraries were size-selected using Beckman-Coulter Ampure XP beads, quantified by qPCR using the Kapa Library Quantification Kit for Illumina (Kapa Biosystems), and checked for sizing by electrophoresis on a BioAnalyzer (Agilent). Completed libraries were loaded on Illumina Truseq Paired-End v2 Cluster Kits and sequenced in 2 x 150 base paired-end runs on an Illumina HiSeq 4000 sequencer with an average of 20 million reads per sample.
GEO
GSM4062026
GEO Accession
GSM4062026