RNA-Seq of Phrynus marginemaculatus: embryonic transcriptome
300ng total RNA input (96-well plate); Poly-A selection; Fragment, Prime, Finish using FPF mix (Frag 5 minutes); First strand cDNA Synthesis; Second strand cDNA Synthesis; Agencourt AMPure XP beads bead purification (lot 16572300); Adenylate 3 Ends Ligation- Dual CD Index (CD plate lot# 20105027); Agencourt AMPure XP beads bead purification (lot 16572300) x2 PCR (15 cycles); Plate removed from thermal cycler followed by quick spin; Agencourt AMPure XP 0.8X bead purification (lot# 16572300);Added 32.5ul RSB & recovered 30ul of purified library; Qubit Quantification- 1:100 dilution (2ul) of library; Qubit HS DNA Rgt Lot# 1941055
SUB7775355
Pmar transcriptome