RNA-Seq of egg duck: adult female egg shell gland
After total RNA was extracted by PEG8000 (Polyethylene glycol 8000), the RNA moleculesin a size range of 1830nt were enriched by polyacrylamide gel electrophoresis(PAGE). Thenthe 3 adapters were added and the 36-44nt RNAs were enriched. The 5 adapters were thenligated to the RNAs as well. The ligation products were reverse transcripted by PCRamplification and the 140-160bp size PCR products were enriched to generate a cDNA libraryand sequenced using Illumina HiSeqTM 2500 by Gene Denovo Biotechnology Co.
SUB4492304
WGC088075-1567-1569