Cascade Hops PacBio CLR Sequel V2.0
Cascade clones grown in greenhouse under controlled conditions. ~100 ug young leaves harvested and placed immediately on ice. DNA extracted with modified Qiagen DNAeasy mini-prep kit. Chemical precipitation and glass hooks substituted for spin columns to prevent shearing. SMRTbell libraries constructed from unsheared DNA with 10kb size selection (BluePippin). Quality assessed with pulse field gels. Sequenced on Sequel with V2.0 chemistry.
SUB6234494
CascadeHops_Sequel_V2.0_30