Illumina HiSeq 2500 paired end sequencing; RNAseq of the small intestine of mice 6 days after they were transplanted with either wildtype allogeneic T cells or glucocorticoid-resistant allogeneic T cells (mouse model of acute graft-versus-host disease
RNAseq of the small intestine of mice 6 days after they were transplanted with either wildtype allogeneic T cells or glucocorticoid-resistant allogeneic T cells (mouse model of acute graft-versus-host disease
RNA was isolated from the the small intestine of mice 6 days after they were transplanted with either wildtype allogeneic T cells or glucocorticoid-resistant allogeneic T cells (mouse model of acute graft-versus-host disease. Mice were sacrificed by carbon dioxide inhalation and snap frozen in liquid nitrogen. RNA was isolated from the the small intestine of 8-12 week-old mice 6 days after they were transplanted with either wildtype allogeneic T cells or glucocorticoid-resistant allogeneic T cells (mouse model of acute graft-versus-host disease. Mice were sacrificed by carbon dioxide inhalation and snap frozen in liquid nitrogen. mice were subjected to a total body irradiation with a dose of 8.5 Gy using an X-Ray source operated at 200 kV, 15 mA and 0.5-mm Cu filtration. The following day, 1x107 BM cells and 2x106 T cells (either wild-type or glucocorticoid resistant) were mixed and injected into the irradiated mice via the tail vein to induce acute graft-versus-host disease Total RNA was isolated using the RNeasy mini kit (qiagen) and  poly adenylated RNA was isolated using oligo d(T) beads prior to reverse transcription Sequencing libraries were prepared using the TruSeq RNA library Prep Kit (Illumina)
MPIMG
E-MTAB-7765:Lck-48_p
Max Planck Institute for Molecular Genetics
E-MTAB-7765:Lck-48_p
Experimental Factor: treatment
transplant with 1x107 bone marrow and 2x106 T cells from c57BL/6 GRlck mice (glucocorticoid resistant)