GSM2564959: O11-WT_ChIPSeq; Zea mays subsp. mays; ChIP-Seq
Endosperm lysates were clarified from sonicated nuclei. O11-DNA complexes were isolated with O11-antibody. o11 mutant seeds were used as negtive control. Libraries were prepared according to Illumina's instructions accompanying the TruSeq ChIP Sample Prep Kit (Catalog# IP-202-1012). ChIP DNA samples were subjected to end repair and A-base addition, followed by ligation with adapters. Barcode sequences of adapters correspond to the following: CTTGTA, 685-wt-1_combined; TTAGGC, 685-mu-1_combined. After adapter ligation DNA was PCR amplified with Illumina primers for 15 cycles and library fragments of ~500 bp (insert plus adaptor and PCR primer sequences) were band isolated from an agarose gel. Illumina Genome Analyzer (GA) sequencing was performed by the OSUCCC Nucleic Acid Shared Resource-Illumina GA II Core.
GEO
GSM2564959
GEO Accession
GSM2564959