WXS of human nonadjacent lung tissue: Sample 1238_Lung
Exon targets isolated using the Agilent All Human Exon v6 and Agilent SureSelectXT Reagent Kit. Genomic DNA (200 ng) was fragmented using a Covaris LE220 focused-ultrasonicator, and libraries prepared and captured on a Sciclone NGSx Workstation. Library size distributions were validated using an Agilent 2200 TapeStation. Additional library QC, blending of pooled indexed libraries, and cluster optimization was performed using Life Technologies' Invitrogen Qubit 2.0 Fluorometer. The resulting libraries were sequenced on an Illumina HiSeq 2500 using a paired-end 100 bp (PE100) strategy.
NCI-phs001805
1238_Lung