GSM3012700: SpVATCtrl_rep2a301; Mus musculus; RNA-Seq
Spleen were mechanically dissociated and visceral adipose tissue were digested with collagenase/dispase to single cell suspensions. Tregs cells were sorted by flow cytometry as DAPI- TCRb+ CD4+ GFP+ cells. Transgenic cells were sorted as TRAV2+ TRBV4+ cells. Single cells were then encapsulated in nanodroplets according to the InDrops protocol. mRNA was reverse transcribed using adapters containing oligo dT in each droplet. The emulsion was then broken and linear amplification was performed by in vitro transcription. Illumina adapters were added by reverse transcription and PCR. Library were sequenced using a NextSeq500.
GEO
GSM3012700
GEO Accession
GSM3012700