GSM2666933: 12_wt; Arabidopsis thaliana; RNA-Seq
Total RNA was extracted using Quick RNATM Mini Prep kit (Zymo Research, Irvine, California, USA). PolyA mRNA sequencing was performed using TrueSeq RNA Library Preparation Kit v2 (Illumina, San Diego, California, USA) according to the manufacturer’s instructions. Briefly, RNA quality and quantity measurements were performed using RNA ScreenTape and Reagents on TapeStation (all from Agilent, Santa Clara, California, USA) and Qubit (Thermo Fisher); only high quality (RIN >8.0) total RNA samples were processed. Next, RNA was DNaseI (Thermo Fisher) treated and the mRNA was purified and fragmented. First strand cDNA synthesis was performed using SuperScript II (Thermo Fisher) followed by second strand cDNA synthesis, end repair, 3’-end adenylation, adapter ligation and PCR amplification. All the purification steps were performed using AmPureXP Beads (Backman Coulter, Brea, California, USA). Final libraries were quality checked using D1000 ScreenTape and Reagents on TapeStation (all from Agilent)
GEO
GSM2666933
GEO Accession
GSM2666933