Microbial diversity associated with copepods in the North Atlantic subtropical gyre
909F-1492R PCR preformed in triplicate, pooled, additional 5 cycles barcoded and Lib-L adapter added as in Berry et al., 2011. Run in triplicate and pooled again. Amplicons purified with AmPure magnetic purification system. Individual samples quantified with picogreen and added in equal concentrations to one tube for sequencing.
University of Massachusetts, Dartmouth
Sargasso Sea bacteria on copepods