GSM3928266: TKO-LPS-3h; Mus musculus; RNA-Seq
RNA was isolated using total  RNA purification kit (GeneMark) and total RNA was polyA enriched. 1.mRNA was isolated, purified and fragmented and cDNA was synthesis 2. The synthesized cDNA was subjected to end-repair and then was 3' adenylated. Adapters were ligated to the ends of these 3' adenylated cDNA fragments 3. cDNA fragments were amplified by PCR with adapters from previous step. 4.The double stranded PCR products were heat denatured and circularized by the splint oligo sequence. The single strand circle DNA (ssCir DNA) were formatted as the final library.
GEO
GSM3928266
GEO Accession
GSM3928266