Iso-Seq of chicken embryo
RNA samples extracted from E1, E3, E5, and E7 embryos were used to prepare the PacBio library. For each developmental stage, equal amounts of total RNA from three biological replicates were pooled together. High-quality RNA samples were used to construct the libraries. Full-length cDNA was produced using the SMARTer PCR cDNA Synthesis Kit. The cDNA was then amplified by 16 cycles of PCR and then size selected using BluePippin (Sage Science, Beverly, MA, USA) into the following bins for each sample: 12kb, 23kb, and 36 kb. These three libraries for each sample were generated using SMRTbell Template Prep Kit 1.0 (Pacific Biosciences, Menlo Park, CA, USA). The 12 libraries were sequenced on Pacific Bioscience RS II platform with 13 SMRT cells.
SUB4472686
E1_1_2K