GSM4064445: KOWT93; Mus musculus; RNA-Seq
Mouse brain cortexes were harvested from Wild-type, Grin1-exon5 knockout and Grin1-exon5 constitutive genotypes and put into RNAlater solution (Ambion). The brain tissues were homogenized in Qiazol using the Tissue Shredder (Qiagen) and total RNA was isolated from using RNeasy Lipid Tissue Mini Kit (Qiagen). Purified RNAs were quantified with a Nanodrop 1000 (Thermo Scientific) and the quality of the RNA assessed using an Agilent Bioanalyzer (Agilent). All RNA used had an RNA Integrity Number > 8.0. RNAs from each mouse contains at least 300 ng of RNA. RNA libraries were prepared for sequencing using standard Illumina protocols.
GEO
GSM4064445
GEO Accession
GSM4064445