GSM1701474: 10pg_HBRR_1; Homo sapiens; RNA-Seq
For samples 1-34 cDNAs were first amplified using a double-random priming strategy followed by library construction with the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB). Briefly, total RNAs was reverse-transcribed with an oligonucleotide used previously for whole genome amplification [multiple annealing and looping-based amplification cycles (MALBAC)] containing a 3' random octamer and a 5' adapter sequence. The same oligonucleotide was used for one round of second strand synthesis generating cDNA fragments that harbor the adapter sequence in a reverse-complementary manner at both ends. These fragments were then amplified by PCR using adapter oligonucleotides to produce sufficient cDNA amounts for generating high-throughput sequencing libraries. For samples 35-37 RNAseq libraries were generated with the NEBNext Ultra RNA Library Kit for Illumina (NEB) with omission of the mRNA isolation step.
GEO
GSM1701474
GEO Accession
GSM1701474