RNA-Seq of Melampsora lini germinated urediniospores -  replicate 3
Total RNA was isolated from up to 200 mg Linum usitatissium  leaves inoculated by Melampsora lini at 8 days post inoculation using the  RNeasy midi kit (Qiagen), according to the manufacturers instructions. Libraries were made and 50 bp single reads  were generated in one lane with samples of Linum usitatissium leaves  inoculated by Melampsora lini at 6 days post inoculation according to  standard Illumina HiSeq protocols. The  sequence reads were analysed according to Australian Genomic Research  Facility quality control measures.  Sequence quality was >95% bases above Q30. The reads were also screened for the  presence of any Illumina adaptor/overrepresented sequences and cross-species  contamination. The primary sequence  data was generated using the Illumina consensus assessment of sequence and  variation (CASAVA) pipeline (version 1.8.2).
SUB5910978
MlgsC