RNA-Seq of Temnothorax rugatulus: brain of nurse from polygyne colony with microgyne queen
At the end of the experiment for each treatment the head of six queens was decapitated and flash frozen using liquid nitrogen. These same was done for one nurse and one forager for the polygynous colonies. Brains were dissected in less than 5 minutes on ice in 1% PBS and immediately crushed into 50 l of Trizol before storage at -80C until extraction. RNA was extracted using the RNAeasy mini extraction kit (Qiagen) and sent to BGI Hongkong for sequencing.
SUB6536676
825-MicRef--Wi-Br