Nanopore WGS of Sphingomonas paucimobilis
Genomic DNA was extracted using a Genomic-tip 500/G kit (Qiagen) and DNA was washed in high salt (1M NaCl / 2mM EDTA) and low ethanol (25%x66.7%), followed by standard ethanol precipitation.  Nanopore sequencing was done with a Rapid Barcoding Kit (SQK-RBK004) and R9.4.1 flow-cell (FLO-MIN106) on a GridION X5 system (Oxford Nanopore Technologies).
SUB7773832
nanopore