Targeted sequencing of Populus deltoides: Sample UF0571
"Genomic DNA was extracted from leaves of 579 P. deltoides individuals, sheared to an average size of 250 bp and barcoded libraries were prepared for sequencing with the Illumina HiSeq 2000 platform. Before sequencing, 12 samples were combined in a pool and approx. 6% of their genome was captured using 204,108 probes to target 18,153 genes (mainly exons) and 23,835 probes to target intergenic regions in 15 Kbp windows across the genome. After capture and sequencing, reads were split by barcode, filtered by quality and aligned to a hybrid P. trichocarpa/P. deltoides reference genome.  "
University of Florida
pool_23_UF0571
alignment_software
Mosaik v2.1.32