rna-seq of mus musculus:CT26_MZCTRL_L2_Rep1
High-throughput sequencing was performed according previous described methods. Berifly, total RNA were extracted from cells using mirVana miRNA Isolation Kit (Invitrogen Cat AM1560). Library creation and sequencing were performed by the Mayo Clinic Genome Analysis Core. For RNA-Seq, 1 ug of total RNA was used to create each sequencing library using Truseq RNA Sample Preparation Kit (Illumina, RS-122-2001). Libraries were then sequenced across 2 lanes on an HiSeq 4000 (Illumina) instrument using 100 base pair paired end mode.
SUB7321935
CT26_MZCTRL_L2_Rep1