Eucalyptus  transcriptome under nutrient stress
RNA was  extracted using PureLink Plant RNA Reagent (Invitrogen) as manufacturer  instructions. To remove any trace of contaminating genomic DNA, RNA elutes  were subjected to a DNase treatment. RNA quantity was measured by  spectrophotometer (Thermo Scientific NanoDrop 1000), and its integrity was  verified by capillary electrophoresis (Agilent Bioanalyzer 2100) using RNA  6000 Nano kit (Agilent Technologies). Only high quality mRNA were converted  into cDNA libraries using Illumina kit (Illumina, Inc.) for a single lane run  in HiSeq 2500 (single-end 51 bp).
SUB5912840
X1C1