RNA-seq of Breast cancer patient: Tumor: Sample b27211b5-7ee1-4e3a-af73-e227b1_D7
Indexed cDNA sequencing libraries were prepared from the RNA samples using the TruSeq Stranded RNA Sample Preparation Kit and bar-coded with individual tags. Library preparation was performed similarly to the WXS. Quality control was performed at every step, and the libraries were quantified using the Agilent 2100 Bioanalyzer. Indexed libraries were prepared as equimolar pools and run on HiSeq4000 (2x150 paired end runs) to generate a minimum of 30 million paired-end reads per sample library. The raw Illumina sequence data were demultiplexed and converted to. fastq files, and adapter and low-quality sequences were trimmed. mRNA sequencing reads were mapped to the human genome hg19 by STAR (version 2.5.3a) using the 1-pass model. The resulting bam was uploaded to short read archive.
NCI-phs000892
296_R
alignment_software
MapSplice version 2.1.8