ATM sequences from U2OS cells treated with canonical TALENs #2
Sequencing #2 from Hubbard et al, 2015.  U2OS cells were nucleofected with a plasmid expressing heterodimeric FokI fusions to the canonical WT ATM-L TALE together with a plasmid encoding the canonical ATM-R TALE–FokI fusion protein. After 48 h, genomic DNA was harvested and the ATM target site and its corresponding known off-target sites OffA1 and OffA23 were amplified from this genomic DNA.  The amplified sites werethen  high-throughput sequenced.  All genomic target sites (ATM on-target, OffA1 and OffA23) were combined together for this one type of TALEN treatment.
Harvard University
U2OS Canonical TALEN #2