P40_72_pairedend
RNA sequencing libraries were prepared for each sample in triplicates (3 biological replicates per sample) at 0hrs, 24hrs and 72hrs post infestation with mealybugs. Total RNA was purified by enriching for mRNA using a TruSeq Stranded mRNA Sample Preparation Kit (Illumina, San Diego, CA, USA). The rRNA species were reduced using Ribo-zero magnetic kit (www.epicentre.com) to produce enriched mRNA. First strand cDNA was synthesized using reverse transcriptase and random hexamers followed by the second strand synthesis. The cDNA fragments were 3′ adenylated followed by adapter ligation with RNA adapter indexes. PCR amplification was performed to enrich purified cDNA template and the library was validated.
ARC-OVI
P40_72