Transcriptome of Pantophthalmus roseni
Messenger RNA (mRNA) was isolated using the Dynabeads mRNA Purification Kit (Invitrogen, Carlsbad, CA, USA) and then sheared using divalent cations at 72°C. These cleaved RNA fragments were transcribed into first-strand cDNA using SuperScript™ II Reverse Transcriptase (Invitrogen, Carlsbad, CA, USA) and N6 primer (IDT). The second-strand cDNA was subsequently synthesized using RNase H (Invitrogen, Carlsbad, CA, USA) and DNA polymerase I (Invitrogen, Shanghai, China). The double-stranded cDNA then underwent end-repair, a single ‘A’ base addition, adapter ligation, and size selection on anagarose gel (250 ± 20 bp). At last, the product was indexed and PCR amplified to finalize the library preparation for the paired-end cDNA. 
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