ChIP-Seq analysis of H3K27me3 in human CD25- CD45RA+ naive cells
Single read - Illumina
BI
DNA_Lib 724
CHIP_PROTOCOL_BEAD_TYPE
Protein A-Sepharose 4B
CHIP_ANTIBODY
H3K27me3
CHIP_PROTOCOL_CHROMATIN_AMOUNT
0.5 to 3 million cells
EXTRACTION_PROTOCOL
cell/nuclei lysis
CHIP_PROTOCOL_ANTIBODY_AMOUNT
~1 ug
CHIP_PROTOCOL_BEAD_AMOUNT
10 ul bed volume
CHIP_PROTOCOL
Bernstein_BROAD_ENCODE_protocol
EXTRACTION_PROTOCOL_SONICATION_CYCLES
1
CHIP_ANTIBODY_CATALOG
07-449
CHIP_ANTIBODY_PROVIDER
Millipore
CHIP_ANTIBODY_LOT
DAM1514011
EXPERIMENT_TYPE
Histone H3K27me3
EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR
Branson
GEO Accession
GSM772911