Eukaryotic community of Zostera marina leaf, Kiel benthocosm tank
Extracted DNA was amplified with primer pairs targeting the V7 region of the 18S rRNA (F-1183mod: 5’-AATTTGACTCAACRCGGG-3’, R-1443mod: 5’- GRGCATCACAGACCTG-3’ (Ray et al., 2016) coupled to custom adaptor-barcode constructs. PCR amplification and Illumina MiSeq library preparation and sequencing (V3 chemistry) was carried out by LGC Genomics in Berlin.
SUB2589022
1183F-1443R-K11D