RNAseq of TN sample at End stage
Total RNA was purified using TRIzol reagent (Invitrogen). RNA purity was checked using the NanoPhotometer spectrophotometer (IMPLEN, CA, USA). RNA concentration was measured using Qubit 2.0 Flurometer (Life Technologied, CA, USA). RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Sequencing libraries were generated using NEBNext UltraTM RNA library Prep Kit for Illumina (NEB, USA) following manufacturers recommendations. The library fragments were purified with AMPure XP system (Beckman Counlter, Beverly, USA). The library preparations were sequenced on an Illumina Hiseq 2500 platfrom and 150 bp paired-end reads were generated(AnoroadBeijing).
SUB7020551
TN-End-5