Targeted Illumina Paired Sequencing of eMERGEseq Variant Gene Panel
Genomic DNA from samples was sheared and processed using hybrid selection and targeted capture with Illumina Rapid Capture probe kits. Libraries were sequenced using Illumina HiSeq 2500 sequencers using 2x76bp paired end sequencing. Sequencing reads were processed through bwa alignment to hg19 (build 37), Picard and GATK pipelines at the Broad Institute.
UW-BIME
SM-B3MIA