RNA-seq of wild-type and 35S::TabZIP60s-MYC transgenic plants before and after heat stress
The total RNA of 1-week old seedlings for both WT and transgenic lines were extracted using TRIzol reagent (Invitrogen) according to the manufacturer’s instructions, which were then sent to the Berry Genomics Co., Ltd (http://www.berrygenomics.com/) for mRNA library construction and deep sequencing using the Illumina HiSeq2500 platform. Before library construction, the quality of RNA samples was examined using an Agilent 2100 Bioanalyzer.
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WT-CK, repicate 1