GSM1701105: testes2; Homo sapiens; RNA-Seq
commerically available Human Testes Total RNA  - Ambion AM7972 Sperm RNA was isolated and purified using a modified RNeasy (Qiagen) protocol and finally the RNA quality assessed for DNA contamination as described in paper A total of 2 ng of unfractionated input RNA was reverse transcribed and the resulting cDNA amplified using SeqPlex RNA Amplification (Sigma-Aldrich Co.) allowing for the use of samples with low quantity of spermatozoa Amplified primer dimers were removed prior to library construction using Agencourt AMPure XP (Beckman Coulter) and libraries were constructed with 50 ng of amplified cDNA using Encore NGS Library System I (Nugen, Inc.). 4bp inline Illumina index primers allowed for multiplexing of samples Paired-end sequencing (2x51  + 6Index) was performed  using an Illumina HiSeq 2500 sequencer
GEO
GSM1701105
GEO Accession
GSM1701105