18S amplicon sequencing of marine metagenome: Bergen, Norway mesocosm
Libraries were prepared and sequenced by the Georgia Genomics and Bioinformatics Core at the University of Georgia. Libraries were constructed to capture either the V4-V5 region of the 16S rRNA using primers 515F (5-GTGYCAGCMGCCGCGGTAA-3) and 926R (5-CCGYCAATTYMTTTRAGTTT-3) or the 18S rRNA gene using primers Euk1391F (5-GTACACACCGCCCGTC-3) and EukBr (5-TGATCCTTCTGCAGGTTCACCTAC-3).  Amplicon libraries were prepared using an input of 25 ng of DNA and sequenced using the Illumina MiSeq platform to produce 300+300 nt paired reads.
SUB5003626
D10_203_1