GSM4338055: IEC_C2: IEC high carb 2; Mus musculus; RNA-Seq
RNA was isolated from sorted T-cells using RNEasy Plus Micro Kit (Qiagen). RNA was isolated from epithelial cells using DirectZol RNA Miniprep Plus Kit with on-column DNAse digestion according to the manufacturer's instructions (Zymo). Library preparation for sorted T-cells performed using SMART-seq2 protocol. Sequencing libraries for epithelial cells were constructed using Illumina TruSeq Library Prep Kit and sequenced on Illumina NextSeq 500 (38 x 38 bp paired-end run).
GEO Accession
GSM4338055