GSM4183026: CRISPRi Library H3 low peak; Homo sapiens; OTHER
Genomic DNA was isolated from FACS-sorter populations using QIAamp DNA Blood Maxi kit. Genomic DNA was PCR amplified with Q5 Hotstart high-fidelity 2X mastermix and barcoding primers for 27 cycles. PCR reactions were purified on QIAquick PCR purification columns and loaded onto a 20% polyacrylamide/TBE gels. A product band ~270 bp was excised and DNA was extracted, quantified and sequenced on an Illumina HiSeq-4000 using custom primers
GEO Accession
GSM4183026