16S rRNA V4 microbiota of channel catfish: Gut_Tank4_Diet3
Two channel catfish were collected from a single tank being fed "Diet3_SoybeanMeal" to access the effects of alternative dietary formulations on the gut microbiota. The entire intestinal tract and its contents were removed from both fish using sterile procedures and were pooled. Samples then homgenized using a sterile chilled mortar/pestle, and a PowerFecal DNA isolation kit was used to isolate DNA. The Fluidigm Access Array PCR platform was used to generate 16S rRNA V4 amplicon library using 515f and 806r PCR primers, indexing barcodes, and Illumina sequencing primers. Library was sequenced on an Illumina MiSeq using v3 chemistry.