Transcriptome sequences of mixed tissue samples from Ranunculus brotherusii
Total RNA was obtained using RNAisoTM Plus (Takara, Qingdao, China) and then treated with RNase-free DNase I (Takara, Qingdao, China) for 45 min according to the manufacturer’s protocols. The quality of total RNA was checked using 2% agarose gel electrophoresis. The RNA samples were then delivered to Beijing Genomics Institute (BGI, Shenzhen, China), and concentrations were checked by Agilent Technologies 2100 Bioanalyzer instrument (Agilent Technologies, Santa Clara CA, USA). The cDNA preparation and Illumina sequencing were performed at BGI. The entire process followed a standardized procedure monitored by BGI’s Quality Control System. The mRNA was isolated from total RNA using oligo (dT) magnetic beads using the manufacturer’s instructions for cDNA library construction. Double stranded cDNA was sequenced using the Illumina HiSeq™ 2000 sequencer (90 bp paired-end)