5'RACE of C. elegans  unc-54(srf0788), unc-54(srf0788) nonu-1(srf0791), unc-54(srf0788)  nonu-1(srf0802)
5'OH end capture with RtcB  ligase and unique adaptor for each sample, pooled samples, reverse  transcription, and nested PCR to prep libraries