GSM1703748: Control_9_UNS; Homo sapiens; RNA-Seq
CD4+ T-cells were then divided into 3 separate groups and stimulated with either 10ul anti-CD3 antibody(CD3 clone HIT3a, 1mg/ml) and 1ul anti-CD28 antibody (CD28 clone CD28.2 ,1mg/ml) for 24 hours at 37°C, 1ug (1mg/ml) Phorbal 12-myristate 13-acetate (PMA) for 4 hours at 37°C or left unstimulated (UNS) to represent basal conditions and incubated for 24 hours at 37°C. RNA libraries were prepared for sequencing using standard Illumina protocols for sequencing on an Illumina HiSeq™ 2000 using 50 base pair single end reads (GATC Biotech, Konstanz, Germany).
GEO Accession
GSM1703748