454 16S rRNA amplicon sequence of North Atlantic archaea: Sample AFH70
Ten liters of seawater were filtered onto 0.2-µm poresize filters (47 mm diameter), flash frozen in liquid nitrogen and stored at -80ºC. DNA was extracted using the UltraClean Soil DNA Isolation Kit (MoBio).The amplification of the V1–V3 region of the 16S rRNA gene was performed by Research and Testing Lab- oratory (Lubbock, TX) with bacterial primers ARC349F (GYGCASCAGKCGMGAAW) and ARC806R  (GGACTACVSGGGTATCTAAT), followed by pyrosequencing by using a Roche 454 GS-FLX system with titanium chemistry.