GSM3612672: SC_GM12878_6UnitDevice5_U1; Homo sapiens; RNA-Seq
MEF cells were harvested at 80% confluence. They were detached by incubating with 0.25% trypsin with 0.1% EDTA (Thermo Fisher 25200056) for 1 min. Cells were centrifuged at 120 × g for 5 min. Then the supernatant was discarded and cells were resuspended in PBS and diluted to a concentration of 3.2 × 10^5 cells/ml. GM12878 cells were harvested in their exponential phase, centrifuged under the same set of conditions and resuspended in PBS and diluted to the same concentration.This solution was then promptly used for single cell capture on the MID-RNA-Seq device. Library preparation was performed using the Nextera XT library preparation kit (Illumina FC-131-1024) using 5 μl of purified RNA following manufacturer's instructions. Bulk RNA-seq samples from a 1000 cells were prepared with Takara Bio SMART-Seq V4 Kit (634894). Libraries were sequenced on Illumina HiSeq 4000/HiSeq X with single-end 50 bp read or paired-end 150 bp reads.
GEO Accession
GSM3612672