GSM4065450: in_R5x: Input RNA Air pollution; Homo sapiens; RIP-Seq
Following exposure, each insert membrane was treated with 1 ml of TRIzol in the apical side and gently mixed to ensure thorough lysis. The whole lysate was collected and frozen until the day of the extraction. TRIzol RNA extraction was conducted following TRIzol's manufacturer instructions. To prevent artificial oxidation of RNA by dissolved oxygen in solutions, ethanol (200 Proof, OmniPur, EMD Millipore, Burlington, MA), isopropanol (molecular biology grade, IBI Scientific, Dubuque, IA) and nuclease-free water (Ambion, Austin, TX) used in the downstream steps after TRIzol were purged with ultra-high purity N2 for 30 min. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM4065450