Tc517_MiSeq_RNA
Mycelium for RNA extraction was grown and harvested on M19 agar (Trione 1969) before being ground in liquid nitrogen. RNA extraction utilized the Zymo Research Fungal/Bacterial RNA Miniprep kit (Zymo Research Corporation, Irvine, CA) per the manufacturer’s instructions. Library preparation for RNA-seq was performed with the Illumina TruSeq RNA v2 kit (Illumina, Inc. San Diego, CA) and sequencing was carried out on an Illumina MiSeq (Illumina, Inc. San Diego, CA) per the manufacturer’s instructions.