Illumina HiSeq 2000 sequencing; eIFA3 - iCLIP
eIFA3 - iCLIP
HeLa cells were cross-linked with 254nm UV. Following the iCLIP protocol the retrieval of protein-bound RNAs and construction of Illumina compatible libraries was done as described in Huppertz et al, 2014. The 3 prime adapter sequence is AGATCGGAAG.
Experimental Factor: immunoprecipitate
antibody eIF4A3
LIBRARY_STRAND
not applicable