MiSeq of Rhizobium leguminosarum bv. trifolii: rpoB, recA, nodA and nodD amplicons form synthetic mixtures of two strains.
DNA from two Rlt strains (SM3 and SM170C) were mixed in various ratios. Individual PCRs using Platinum taq for rpoB, recA, nodA and nodD  Rlt genes were pooled for each sample.