GSM2064534: S1511_chronic_VCV_067; Rattus norvegicus; RNA-Seq
Lung tissue was removed, flash frozen in liquid nitrogen and RNA was harvested using Qiagen RNeasy kit. mRNA was isolated using a bead-based poly-dT selection technique  (Sera Mag Oligo(dT) beads, Thermo Scientific). Library preparation was performed using NEBnext reagents (New England Biolabs). Total RNA was isolated and quality controlled using the Bioanalyzer (Agilent). mRNA from 1 ug total RNA was isolated using bead based poly-dT selection followed by chemical fragmentation and further processing for Illumina short read sequencing. For strand specific sequencing the dUTP method according to Parkhomchuk et al. (Parkhomchuk D, Borodina T, Amstislavskiy V, Banaru M, Hallen L, Krobitsch S, Lehrach H, Soldatov A. Transcriptome analysis by strand-specific sequencing of complementary DNA. Nucleic Acids Res. 2009 Oct;37(18):e123) was applied using the NEBnext chemistries from NEB. All samples were barcoded and sequenced as a pool using the Hiseq 2000 platform from Illumina
GEO Accession
GSM2064534