RNA-seq of OsDT11
Oligo (dT) magnetic beads are used to select mRNA with polyA tail, Then obtain the target RNA after purification.  Fragment the target RNA and reverse transcription to double-strand cDNA (dscDNA) by N6 random primer. End repair the dscDNA with phosphate at 5' end and stickiness 'A' at 3' end, then ligate and adaptor with stickiness 'T' at 3' end to the dscDNA. Two specific primers are used to amplify the ligation product.