Evaluation of sgRNA library generated by EATING method from E.coli (XL1-Blue) genome - replicate 2 (lane 2)
sgRNA library was generated by EATING method from XL1-Blue E.coli genomic DNA. Replicate 2 was converted to Illumina sequencing library using PrepX Complete ILM DNA Library Preparation Kit (WAFERGEN Biosystems, cat. no. 400075). For amplification and barcode (ACTTGA) addition, 9 cycles of PCR were performed. Sequencing library synthesis was performed by QB3 Functional Genomics Laboratory (FGL) at UC Berkeley. Sequencing was performed by QB3 QB3 Vincent J. Coates Genomics Sequencing Laboratory (VC GSL) at UC Berkeley (ADL_2_index8_ACTTGA_L002_R1_002.fastq and ADL_2_index8_ACTTGA_L002_R1_001.fastq).