GSM1892394: H3K9Ac_ChIPSeq naïve 1; Drosophila melanogaster; ChIP-Seq
Heads were collected and homogenized in crosslinking buffer. The nuclei were dissolved in extraction buffer, sonicated to dissociate the individual nuclei, and precipitated with ANTI-FLAG M2 Affinity Gel. The precipitates were rinsed 4 times in extraction buffer, with 5 min nutation at 4℃ between washes, and subjected to ChIP analysis. The purified mushroom body nuclei were sonicated, resulting in fragmentation of DNA ranging from 100 bp to 500 bp. The extracts were centrifuged to remove insoluble materials including the M2 Affinity Gel, and the supernatants were used for immunoprecipitation with specific antibodies. The ChIP DNA samples prepared were used to prepare a library for SOLiD5500, according to the manufacturer's instructions.
GEO Accession
GSM1892394