Genomic characterization of colistin heteroresistance in Klebsiella pneumoniae during a nosocomial outbreak
Genomic DNA of K. pneumoniae isolates were isolated from overnight cultures, grown in Luria Broth at 37 ℃ with shaking at 250 rpm, using the Wizard Genomic DNA purification kit (Promega, Madison WI) according to the manufacturer’s instructions. Sequence libraries were prepared using the Nextera XT kit (Illumina, San Diego CA), according to the manufacturer’s instructions. Libraries were sequenced on Illumina’s MiSeq system, using the 500 cycles (2x250 bp) MiSeq Reagent kit v2.