1036_SCRN
The fungal ITS-1 region was amplified by PCR and sequenced using a 2x300 bp paired-end protocol on the MiSeq platform (Illumina). All libraries were subjected to quality control using qPCR, DNA 1000 Bioanalyzer (Agilent), and Qubit (Life Technologies) to validate and quantitate library construction prior to preparing a Paired End flow cell.