HEK293T cells transfected with the CBX7 expression vector
Total CBX7-binding RNAs in the HEK293T cells transfected with the CBX7 expression vector are immunoprecipitated and extracted using CBX7 antibody (Ab21873, Abcam, UK). cDNA is synthesized from the RIPed-RNAs using random primers. The cDNA is then used to construct a library for Next Generation Sequencing with the forward primer (1¡Á50; Illummina HiSeq 2500) by Shanghai Biotechnology Corporation. Raw reads are obtained and cleaned for human genome mapping and peak detection.