GSM2406788: input_UNT_2; Homo sapiens; ChIP-Seq
U2OS cells were cross-linked in medium containing 1% formaldehyde for 10 min at room temperature, neutralized with 0.125 M glycine, scraped off and rinsed twice with PBS. Pellets were resuspended in 10 ml of buffer L1 (50 mM Hepes KOH, pH 7.5, 140 mM NaCl, 1 mM EDTA pH 8.0, 10% glycerol, 5% NP-40, 0.25% Triton X-100) and incubated for 10 min at 4°C. Cells were spun down, resuspended in 10 ml of buffer L2 (200 mM NaCl, 1 mM EDTA pH 8.0, 0.5 mM EGTA pH 8.0, 10 mM Tris pH 8.0) and incubated for 10 min at room temperature followed by centrifugation and resuspension in 900 μl buffer L3 (1 mM EDTA pH 8.0, 0.5 mM EGTA pH 8.0, 10 mM Tris pH 8.0, 100 mM NaCl, 0.1% Na-deoxycholate, 0.17 mM N-lauroyl sarcosine, supplemented with protease inhibitors). Library was prepared with Ovation Ultralow Library Systems v2  (NuGEN) according to manufacturer's instructions.
GEO Accession
GSM2406788