GSM3991536: Thoracic Aorta, Tsc1−/− (iii); Mus musculus; RNA-Seq
Thoracic aortas were rapidly excised, flash frozen in liquid nitrogen, crushed tissue was immersed in RLT lysis buffer (Qiagen) and vigorously vortexed, total RNA was isolated using a RNeasy Mini Kit and DNase Digestion Set (Qiagen) according to the manufacturer's protocol, and 500 ng of intact total RNA (RIN>7) was used for polyA selection and construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM3991536