GSM2830580: Gibeaux201611_XenopusHybrid_TELSs42; Xenopus tropicalis x Xenopus laevis; RNA-Seq
Embryos were homogenize mechanically in TRIzol (Thermo Fisher Scientific, Waltham, MA) using up to a 30-gauge needle and processed according to supplier instructions. After resuspension in nuclease-free H2O, RNAs were cleaned up using RNeasy kit (Qiagen Inc.) with on-column DNA digestion, following supplier protocol.  mRNA enrichment was performed on total RNA using polyA selection with the Invitrogen Dynabeads mRNA Direct kit. The library preparation was done on the Apollo324™ with PrepX™ RNAseq Library Prep Kits (WaferGen Biosystems, Fremont, CA), and 13 cycles of PCR amplification was used for index addition and library fragment enrichment.
GEO Accession
GSM2830580