GSM4498290: NURF301BAP170_ATACseq_Rep1; Drosophila melanogaster; ATAC-seq
For ATAC-seq, we counted and aliquoted 10e5 cells and washed them with ice-cold PBS. Cells were then lysed on ice for 3 min in ATAC-lysis buffer (10 mM Tris-Cl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% NP-40, and 1x Pierce Protease Inhibitors). Nuclei were transposed with Illumina Nextera adapter sequences with 1.5 µL lab-made Tn5 transposase in TD buffer (10 mM Tris-Cl pH 7.4, 10% DMF, and 5 mM MgCl2) for 30 min at 37 °C, and transposed gDNA was recovered by phenol:chloroform extraction followed by ethanol precipitation. Libraries were amplified for 11 PCR cycles using Illumina Nextera indexed primers.
GEO Accession
GSM4498290