GSM3612802: Parental Replicate 2; Homo sapiens; RNA-Seq
RNA was extracted from BRCA2 wild-type or heterozygous mutant clones using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions.  RNA samples were then treated with Ambion Turbo DNAse (Thermo Fisher Scientific, Waltham, MA), and DNA contamination was confirmed to be <10% for all samples.  RNA quantity was determined using the Qubit RNA Assay Kit (Thermo Fisher Scientific) and RNA quality was determined on an Agilent Bioanalyzer using the RNA Pico Kit (Agilent, Santa Clara, CA).  Using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA), 50-100 ng of total RNA was converted into a DNA library following the manufacturer's protocol. Following library construction, DNA libraries were assessed for quality control.  Library quantity was determined using the Qubit High Sensitivity DNA Kit (Thermo Fisher Scientific) and library size was determined using the Bioanalyzer High Sensitivity Chip Kit (Agilent).
GEO Accession
GSM3612802