GSM2065764: Osr1 GCE/+ Rep 2; Mus musculus; RNA-Seq
Limbs of E13.5 Osr1 GCE/+ and Osr1 GCE/GCE embryos were extracted, minced and digested by using a collagenase solution. Cell suspensions were stained with propidium iodine. GFP-expressing live cells were sorted by using a FACS Aria II and collected directly into RLT buffer for RNA extraction (RNeasy mini kit, Qiagen). RNA libraries were prepared by using the TruSeq RNA Library Preparation Kit v2 according to Illumina's intructions and subjected to high-throughput sequencing by using the Illumina HiSeq 2500 technology. 65-75 million of 50-bp paired-end reads were generated with a mean insert size of 150 bp.
GEO Accession
GSM2065764