GSM1620018: ego-1; Caenorhabditis elegans; miRNA-Seq
Total RNA extracted using Trisure (BioLine) or Trizol (Invitrogen) according to manufacturer's instructions and followed by an overnight precipitation at -20C using isopropanol and glycogen as a carrier.  For RIP, custom Anti HRDE-1 polyclonal antibody was generated by Genomic Antibody Technology(GAT) by SDIX (Newark USA) against amino acids 12-161 of HRDE-1. Before library preparation, 1-5ug of total RNA was treated with 1ul of RNA polyphosphatase (Epicentre) for 30 minutes at 37C.  RNA was then extracted using Phenol/chloroform and precipitated overnight at -20C using Ethanol and Sodium Acetate.  Following this we followed the Illumina Truseq small RNA protocol according to the manufacturer's instructions
GEO Accession
GSM1620018