3 hour foot regenerating head set 2
"Hydra magnipapillata RNA was isolated from polyps regenerating head from foot.RNA was isolated from ~100 hydra regenerating head from foot region at time 3hours using Trizol as per manufacturer's instructions. Five micrograms of total RNA was used to generate small RNA libraries using TruSeq small RNA kit from Illumina as per manufacturer's protocol. Briefly specific 5' and 3' adapters were ligated to RNA; ligated products were reverse-transcribed and amplified by polymerase chain reaction (PCR). PCR products were separated using polyacrylamide gel electrophoresis, and products corresponding to adapter-ligated 18-35-nt-long RNAs (140-160 bp) were eluted from the gel."