GSM1621350: Osmotic Stress Time 60 F rep2; Saccharomyces cerevisiae; OTHER
5 million cells were washed once in Sorbitol buffer (1.4 M Sorbitol, 40 mM HEPES-KOH pH 7.5, 0.5 mM MgCl2) + 10 mm DTT + 0.6 M NaCl before being incubated for 5 min at 30 degrees and shaking at 300 rpm with 0.5 mg/mL 100T zymolyase.  The cells were then washed once in Sorbitol Buffer + 0.6 M NaCl before being incubated with 2.5 ml of Nextera Transposase in 47.5 ml of 1x TD buffer at 37 degrees shaking at 300 rpm for 15 minutes. Sequencing libraries were constructed using a modified version of the Illumina Nextera DNA Sample prep kit, as per Buenrostro et al. (2013).
GEO Accession
GSM1621350