Illumina MiSeq paired amplicon sequencing studying rearranged T-cell receptor beta (TCRB) genes from cDNA in sampleDonor9_CD4M_1_bc111
To amplify rearranged T-cell receptor beta (TCRB) genes from cDNA, 39 forward primers, each specific to one or two functional TCRBV segments, and one reverse primer specific to the CB segments were designed based on the repertoire of TCR germline segments present in the ImMunoGeneTics (IMGT) database (www.imgt.org) (Table S3). A 12-bp nucleotide unique barcode sequence was inserted in the reverse primer between the CB sequence and Illumina universal reverse sequence.