RNA-Seq of varicose vein: adult male
The main procedure for library construction included mRNA concentration, fragmentation, cDNA synthesis, end repairing, add A-tailing and adapter, fragments selection and library purification. The reagent used included NEBNext super speed RNA Library Prep Kit for Illumina; Beckman AM Pure XP beads, Beckman; 80% Ethanol; Promega magnetic frame PCR instrument, ABI 9700.