18S amplicon sequencing of human stool sample
The V4 region of the 18S rRNA gene was amplified from the DNA by using primers 574*F and 1132R targeting microeukayotes. 25 amplification cycles were employed. Sequencing with 2 x 300 nt was performed using the V3 MiSeq kit on a MiSeq platform (Illumina). Amplification and sequencing was performed by the Groupe Interdisciplinaire de Gťnoprotťomique Appliquťe (GIGA; LiŔge, Belgium).