GSM4066621: NFsi_rep3; Homo sapiens; RNA-Seq
Samples were isolated using the TRIzol™ Reagent (ThermoFisher Scientific, 15596026). Fragmented RNA were subjected to first strand and second strand cDNA synthesis following by adaptor ligation and enrichment with a low-cycle according to instructions of NEBNext® Ultra™ RNA Library Prep Kit for Illumina®.The purified library products were evaluated using the Agilent 2200 TapeStation and Qubit®2.0(Life Technologies, USA) Libraries were sequenced on an Illumina HiSeq™ 3000 platform with 6G base PE150 reads.
GEO Accession
GSM4066621