GSM2066265: wt_DNA_mnase.bam; Mus musculus; MNase-Seq
Genomic DNA was isolated from sperm by proteinase K digestion and recovered by phenol-chloroform extraction and subsequently precipitated with ethanol. Purified DNA was digested with micrococcal nuclease. DNAs were used to construct multiplexed DNA sequencing libraries with the NEBNext ChIP-Seq kit (New England BioLabs). All libraries were subjected to 50 cycles of paired-end sequencing on the Illumina HiSeq 2500 platform.
GEO Accession
GSM2066265