16S rRNA gene amplicon sequencing of chicken metagenome
Genomic DNA was isolated from checken eggshells, fecal swabs, and intestinal lumen and mucosa samples using the MagAttract PowerMicrobiome 96-well DNA/RNA kit following the manufactures instructions. The V4 hypervariable region of the bacterial 16S rRNA gene was PCR amplified and sequenced using the paired-end method on the MiSeq platform