Bacterial and archaeal 16S gene amplicon sequencing of durum wheat (Triticum durum) bulk soil: 7 months after the inorganic N fertilization
Metagenomic DNA (mDNA) from soil samples was extracted using  the Mo Bio PowerSoil DNA Isolation kit. Amplicons were prepared from total mDNA by PCR targeting the V4 hypervariable region of the bacterial and archaeal 16S rRNA gene using the modified primers 515f: 5'-GTGYCAGCMGCCGCGGTAA-3' and 806r: 5'-GGACTACNVGGGTWTCTAAT-3' (Parada et al., 2015; Apprill et al., 2015). PCR amplicons were quantified using PicoGreen, normalized at 1.5 ng l-1, pooled together and sent for sequencing on an Illumina MiSeq (paired-end 2x250) at the McGill University and Genome Qubec Innovation center (Montreal, Canada).