GSM2486186: cocktail rep2; Mus musculus; RNA-Seq
Brain slice hemispheres that underwent incubation protocol in cocktail or control aCSF, were placed under a dissection microscope and auditory cortices dissected while submerged in bubbled aCSF. Total RNA from dissected auditory cortices was isolated by Trizol (800µl / auditory cortex; Life technologies, Grand Island, NY, USA). One microgram of total RNA was used for poly-A selection with a Dynabeads mRNA purification kit (Invitrogen, CA, USA) followed by cDNA generation (NEB 1st strand and NEB 2nd strand ultra directional Kits; New England BioLabs, Frankfurt am Main, Germany). Libraries were prepared using a NEBNext Ultra DNA kit (New England BioLabs, Frankfurt am Main, Germany) and quantified with a Library Quantification kit for Illumina platforms (Kapa Biosystems, Inc., Wilmington, MA, USA).
GEO Accession
GSM2486186