Illumina Genome Analyzer IIx sequencing; Cavendish banana fruits 90 days after flowering (DAF) untreated, 5 days after harvest
Banana fruits (cv. Cavendish, AAA triploid) were harvested at three developmental stages, namely 40, 60 and 90 DAF. After harvest, fruits were kept at 20C for 24 h and at least 10 fruits per development stage were subjected to treatment with 10,000 ppm of acetylene (an ethylene analogue) for 1 day, followed by 4 days storage in air. The control fruits were kept for 5 days in air at 20C.
cDNA libraries were constructed using the SMARTer PCR cDNA Synthesis Kit (Clontech, Saint-Quentin-en-Yvelines, France) according to the manufacturer's instructions, using 1 ug of DNase-treated total RNA. The cDNAs were sonicated to a 150- to 600-bp size range using the S2 Covaris instrument (Covaris, Inc., USA). Single end libraries were prepared using standard Illumina protocols (Illumina DNA sample kit). Each library was sequenced using the 76-base length read chemistry in a single flow cell on the Illumina GA IIx (Illumina, USA).