GSM3990099: LS-166_BMS; Homo sapiens; RNA-Seq
Total RNA from human cell cultures was obtained using RNeasy kit (QIAGEN) and quality checked by QIAxpert spectrophotometer (QIAGEN) prior to cDNA synthesis with 200 ng RNA input using a high-capacity cDNA reverse transcription kit (Applied Biosystems). Sample cDNA was analyzed by Lightcycler480 (Roche) quantitative real-time PCR using SYBR Green dye (Roche) and target primers (Table S3). Relative mRNA expression was determined by standard curves and normalized to reference genes (Table S3) as indicated. qPCR was performed with duplicate technical reactions per biological sample and averaged for further analyses. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM3990099