Endophytes in urban trees
The 16S rRNA amplicons were initially generated by carrying out PCR reactions using 16S universal Eubacterial primers (27F and 1492R). However in addition to the primers, plastid and mitochondrial oligonucleotide blockers were added to the PCR reaction at a final concentration of 0.5 μM, to minimize amplification of plastid and mitochondrial sequences. The resultant amplicons were size selected (for ~ 1500bp) and used to prepare the libraries for sequencing on Roche 454 FLX titanium instrument.