GSM2907101: Quad2; Homo sapiens; ssRNA-seq
Cells were lightly trypsinzed and centrifuged to remove media. Total RNA was purified using the RNeasy mini kit (Qiagen). The quality of total RNA was validated by Bioanalyzer (Agilent Technologies). Approximately 1 µg of RNA per sample was used to construct the cDNA sequencing library using TruSeq stranded mRNA library prep kit (Illumina), followed by quantitation using KAPA library quantification kit for Illumina platforms (Kapa Biosystems).
GEO Accession
GSM2907101