Illumina HiSeq 2000 sequencing; qiita_ptid_65:2080.S30D215
In April-May 2012, we participated in a research cruise as part of the US CLIVAR/CO2 Repeat Hydrography program, onboard the UNOLS ships R/V Atlantis. The cruise was a repeat of World Ocean Circulation Experiment (WOCE) line A20 (longitude 52W), previously conducted in 2003. 10-L water samples were collected at a total of 83 stations at varying depth intervals, using a 36-Niskin bottle rosette. We took 0.5-1.0 L samples from the rosette roughly once per day at 16 stations throughout the cruise. At each station, water was taken from at least three distinct zones in the water column, based on data from the CTD: the middle of the mixed layer, the local oxygen minimum, and the middle of the bathypelagic zone. At 7 stations, samples were also drawn from the bottom-most bottle. Biomass was immediately collected on 0.2 um filters under vacuum filtration. SIP microcosms were set up at 13 stations using 13C sodium acetate, 13C urea, 13C sodium bicarbonate, 13C algal lipid/pigment extract, and 15N algal protein extract. The samples submitted to the Earth Microbiome Project represent T=0 time points for the SIP microcosms (before amendment) and extra samples taken for RNA/DNA analysis. Results from SIP are currently being processed.
EMP V4 515f,806rbc protocol
barcode
TGTATCTTCACC
center_name
ANL
center_project_name
Seyler_North_Atlantic_water_column
emp_status
EMP
illumina_technology
MiSeq
linker
GT
pcr_primers
FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
primer
GTGTGCCAGCMGCCGCGGTAA
run_center
ANL
run_date
2013
run_prefix
Undetermined_S0_L001_R1
samp_collect_device
niskin bottle
samp_mat_process
OBI:liquid filter function
samp_size
0.5L
sample_center
ANL
sequencing_meth
sequencing by synthesis
study_center
CCME
target_gene
16S rRNA
target_subfragment
V4