GSM4560461: Choroid Plexus Org D27; Homo sapiens; RNA-Seq
Single cell dissociation was performed with Accumax _Dnase and Actinomycin D for 15-20 min at 37C with periodic flicking and then trituration every 5 min. Cells were filtered through a 50um filter and dead cells removed using Miltenyi Dead Cell Removal kit before running approximately 16,000 cells per sample lane through the 10X Genomics Chromium Single Cell 3' Library & Gel Bead Kit (10X Genomics, 120237) workflow. The reverse transcriptase reaction and subsequent amplification was carried out on a Thermal Cycler according to 10X Genomics manufacturer instructions. Libraries were quality tested before sequencing using a 2100 Bioanalyzer Instrument (Agilent). Each sample was loaded onto separate wells of the 10X Chromium system and then pooled for subsequent sequencing on two lanes of an S1 flowcell on the Illumina Novaseq 6000.
GEO Accession
GSM4560461