Nanopore shotgun DNA sequencing of whole Letharia columbiana thallus
DNA was extracted from a single Letharia lupina thallus using the Qiagen DNeasy Plant Pro kit. Ten extractions from the same thallus were pooled and size selected with magnetic beads, then prepared for sequencing using the Nanopore library kit LSK109. The library was then sequenced on a single MinION flow cell (r9.4.1). Reads from the "pass" folder were then basecalled with guppy version 3.6.0 (note, they are therefore slightly more accurate than those used in the publication)