Lachancea waltii Genomic Library
L. waltii genomic DNA obtained from logarithmically growing cultures was randomly sheared by sonication.  Sheared DNA was end-repaired and run through a gel to size select 350 and 1,000 base pair fragments.  Fragments were cloned into a yeast centromeric plasmid that otherwise lacks an origin of replication.  Plasmid inserts were amplified from the plasmid by PCR using primers containing Illumina sequencing adapters.