Bacterial Communities and Possible Human Pathogens in Domestic Refrigerator Revealed by a Next Generation Sequencing
Inner of 4 degree and -20 degree in refrigerator was swabbed by two sterile cotton pledget. The head of each swab was aseptically cut from the handle, put into the PowerBead Tubes. DNA extraction was done follow the instruction. V4-V5 of 16s rDNA was amplified with primer 515F and 907R.