GSM2752339: untreated CTLL2 cells 4 hr rep 2; Mus musculus; RNA-Seq
Total RNA was isolated from CTLL2 cells by TRIzol reagent extraction (Thermo Fisher Scientific). Prior to RNA-Seq library preparation, RNA quality and integrity was determined using the Agillent Bioanalyzer 2100. Only RNA with RIN values >8.0 were used for the experiment. RNA-Seq libraries were built with Illumina's TruSeq RNA Kit V2 (RS-122-2001) as per manufacturers protocol. All libraries were indexed in such a way as to ensure only one index/lane on the HiSeq. Finished libraries were quality checked with a High Sensitivity DNA chip on the Bioanalyzer to determine average size and quantified on a Qubit with high sensitivity DNA reagents. The libraries were then sent to the Marshall Genomics Core Facility where they were pooled in equimolar ratios and run on three lanes of an Illumina HiSeq1500 in a 2 x 50 base paired end design yielding a minimum of 49.1 million reads per sample.
GEO Accession
GSM2752339