GSM3611332: Md-tra-RNAi-treated genotypic female abdomen replicate 1 (sex-reversed males); Musca domestica; RNA-Seq
Abdomen and head was dissected from individaul flies and frozen in liquid nitrogen. RNA was extracted from the tissue samples from the individual flies with the NucleoSpin RNAII kit following the manufacturer's protocol including DNA digestion steps. The Illumina TruSeq Stranded mRNA Sample Preparation Kit was used with 1.5ug (1.2ug for GFP-female-H2) of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM3611332