GSM3389278: KO NT2; Mus musculus; RNA-Seq
Total RNA was extracted using the Trizol reagent (Invitrogen). Contaminating DNA was removed by DNaseI (Ambion) treatment, and the RNA was further purified using QIAGEN RNeasy Kit. Cellular RNA was extracted using the RNA extraction protocol mentioned in previous section. The quality of extracted RNA samples was accessed by qRT-PCR. mRNA libraries were prepared using the TruSeq Stranded mRNA Library Prep Kit (Illumina, RS-122-2101), following the manufacturer's protocol.
GEO Accession
GSM3389278