GSM1519715: The ribonucletide incorpration due to DNA synthesis by Pol-delta; Schizosaccharomyces pombe; OTHER
Genomic DNA was alkaline treated and fragments of 300-500 bp of the ssDNA were isolated from agarose gel. ssDNA was converted to dsDNA using the BioPrime DNA Labelling system (invitrogen) with dTTP substituted by dUTP. dsDNA was used for Illumina library preparation using NEBNext® Ultra™ DNA Library Prep Kit with the following modified protocol: end-cleaning of DNA fragments and adaptor-ligation was performed without USER treatment followed by size selection of insert (250 - 600 bp) using AMpure XP beads. Purified DNA was treated with USER enzyme and subsequent PCR (13 cycle) using multiplexing index-primers generated Illumina libraries. DNA was prepared using the QIAGEN 100/G Genomic-tip"
GEO Accession
GSM1519715