Targeted CRISPR assay for MHC - Run1 (more barcodes), second alignment with phasing
MHC CRISPR assay to enrich 4Mb target region using SAGE-HLS instrument; two separate gRNA pools (Set 1 and offset Set 2); 10X Chromium WGS library preparation using 90 ul fraction of 120 ul total droplets to barcode short-read library; phased again with a downsampling