GSM3990101: ChIPseq of Pir2; Schizosaccharomyces pombe; ChIP-Seq
Cells were crosslinked with 1% formaldehyde at room temperature for 20 minutes. Cells were lysed and sonicated using Bioruptor. Immunoprecipitations were performed using GFP antibodies (ab290). Crosslink was reversed overnight at 65˚C, and treated with with RNase A and proteinase K. QIAquick PCR purification kit (cat. no. 28014) was used to purify the DNA. NEBNext Ultra II DNA Library Prep kit (NEB, E7645S) was used according to manufacturer's recommendation. Libraries were analyzedanalyzed using an Agilent 4200 TapeStation system (Agilent) and sequenced on the Illumina NextSeq 500 platform.