Draft genome sequence of Williamsia sp. BULT 1.1 isolated from semi-arid grassland soil from South Africa
The initial concentration of DNA was evaluated (8.76 ng/µL) using the Qubit® dsDNA HS Assay Kit (Life Technologies). The library was prepared using Nextera DNA Sample preparation kit (Illumina) following the manufacturer's user guide. 50 ng DNA was used to prepare the library. The sample underwent the simultaneous fragmentation and addition of adapter sequences. These adapters are utilized during a limited-cycle (5 cycles) PCR in which unique indices were added to the sample. Following the library preparation, the final concentration of the library (4.06 ng/µL) was measured using the Qubit® dsDNA HS Assay Kit (Life Technologies), and the average library size was determined using the Agilent 2100 Bioanalyzer (Agilent Technologies).  The library was diluted (to 10.0pM) and sequenced paired end for 500 cycles using the HiSeq system (Illumina).