GSM1707667: Small RNA short-time; Gossypium hirsutum; miRNA-Seq
Total RNA was isolated using a modified CTAB method and purified using Qiagen RNeasy columns (Qiagen, Hilden, Germany).Then,total RNA was separated by 15% denaturing polyacrylamide gel electrophoresis (PAGE) to recover the population of small RNAs. The extracted small RNAs then was dephosphorylated using alkaline phosphatase and ligated to 5'- and 3'-RNA adaptors by T4 RNA ligase. The adaptor-ligated sRNAs were subsequently transcribed to single-stranded cDNA using SuperScript II Reverse Transcriptase. After polymerase chain reaction (PCR) amplification, PCR products were purified and subjected to SBS sequencing with a Solaxa genome analyzer II at the BGI (Beijing Genomics Institute, Shenzhen, China).
GEO Accession
GSM1707667