GSM3929868: ctrl_rep5; Mus musculus; RNA-Seq
Full-length cDNA of four pooled oocytes was generated using the SMART Seq v4 Ultra Low Input RNA kit for Sequencing (Takara Bio, Mountain View, CA, USA) according to the manufacturer's instructions. The template switching activity of reverse transcriptases to enrich full-length cDNA as well as the addition of defined PCR adapters directly to the ends of the first strand cDNA define the SMART technology. Amplified cDNA was purified using Agencourt AMPure XP Beads (Beckman Coulter). The obtained full-length cDNA was validated using the Qubit™ fluorimeter (Thermo Fisher Scientific). The RNA-Sequencing libraries were generated using the tagmentation technology of the NexteraXT Kit (Illumina) following the manufacturer's instructions. Libraries were purified using Agencourt AMPure XP Beads (Beckman Coulter) and were quantified on the Bioanalyzer.
GEO Accession
GSM3929868