GSM1621337: S. pombe_5M_20min; Schizosaccharomyces pombe; OTHER
5 M Cells were washed twice in Sorbitol Buffer  (1.4 M Sorbitol, 40 mM HEPES-KOH pH 7.5, 0.5 mM MgCl2)  + 10 mm BME, incubated in Sorbitol Buffer + 2mm BME for 30 minutes, and the incubated in Sorbitol buffer with 0.25 mg/mL 100T Zymolyase for 23 minutes, and then washed twice in Sorbitol Buffer before incubated with 2.5 μl of Nextera Transposase in 47.5 μl of 1x TD buffer at 37 degrees for 30 minutes. Sequencing libraries were constructed using a modified version of the Illumina Nextera DNA Sample prep kit, as per Buenrostro et al. (2013).
GEO Accession
GSM1621337