whole exome sequencing of LKB1 mutant lung tumors
Total genomic DNA were extracted using DNeasy Blood & Tissue Kit (Qiagen) according to manufacture manual. Mouse exome libraries were prepared using Roche SeqCap EZ mouse exome kit (54 Mb, catalog number L2RD02), starting with 250 ng of genomic DNA, following the manufacturers protocol (Nimblegen SeqCap EZ Library SR, version 5.1).