GSM3543638: 13_1550_3-3_35CR-ENZR_SPT_D5; Homo sapiens; RNA-Seq
RNA extraction was performed using RNA STAT 60 (Tel-Test, Friendswood, TX) and RNeasy Mini kit, followed by DNase digestion in solution prior to purification (Qiagen, Germantown, MD) for RNA-seq and quantitative PCR analyses. RNA integrity number was determined using the Agilent Bioanalyzer (Agilent, Santa Clara, CA). RNA-seq libraries were constructed from 1 ug total RNA using the Illumina TruSeq mRNA LT Sample Prep Kit according to the manufacturer's protocol. Barcoded libraries were pooled and sequenced on the Illumina HiSeq 2500 generating 50 bp paired end reads.
GEO Accession
GSM3543638