RNA-Seq of Littorina saxatilis: adult female mantle
RNA was extracted from the fresh tissues using Tri reagent followed by a treatment with DNAse Turbo and a clean-up with the Qiagen RNEasy Mini clean-up kit according to the manufacturers protocol. The RNA samples were frozen at -80 C in liquid nitrogen and shipped to BGI (Shenzhen, China) for cDNA library preparation and paired-end sequencing was done on an Illumina HiSeq 2000 instrument.