Labyrinthulomycetes 18S library from PICO time series: 2013-12-04
The DNA was extracted through physical lysis by bead beating, phenol-chloroform extraction coupled with RNase treatment, and isopropanol precipitation followed by PCR inhibitor removal. Partial Labyrinthulomycetes 18S rRNA genes were amplified using dual index primers LABY-A (5'-GGGATCGAAGATGATTAG-3') and LABY-Y (5'-CWCRAACTTCCTTCCGGT-3').