pnp1 replicate 1
Total RNA was extracted using the RNeasy Plant Mini Kit (Qiagen, http://www.qiagen.com) from two WT and two pnp1-1 samples. Ten µg total RNA were depleted of rRNAs using the plant RiboMinus-Kit (Invitrogen, http://www.invitrogen.com). Strand-specific RNA-Seq libraries were prepared following the 'Directional mRNA-Seq Library Prep Pre-Release' protocol by Illumina.