Illumina RNA Sequencing of Sample PN12 for study "Low mutation burden and frequent loss of CDKN2A/B and SMARCA2, but not PRC2 in type 1-associated atypical neurofibromas"
Illumina 101 base paired-end sequencing of sample PN12 on HiSeq2500 using version 4 flow cells and reagents.  One microgram of total RNA was depleted of rRNAs using RiboZero GOLD. Double-stranded cDNA was sheared to ~175 base fragments using a Covaris E210 and libraries were created using TruSeq RNA Kit ver.2.