RNA-Seq isolated from whole liver from Lgr4 and Lgr5 double KO mice at 48h after partial hepatectomy (PH).
RNA sequencing libraries were prepared from mouse liver total RNA using the Illumina TruSeq Stranded Total RNA sample preparation protocol following the manufacturer instructions (Illumina, Part #15031048 Rev. E, October 2013). As part of this procedure, both cytoplasmic and mitochondrial rRNA were removed with the Ribo-Zero Gold Kit. Each library was sequenced in paired end mode, 2x76bp, using the HiSeq2500 sequencer following the manufacturer’s protocol. Images from the instrument were processed using the manufacturer’s software to generate FASTQ sequence files. Read quality was assessed by running FastQC (version 0.10) on the FASTQ files. Sequencing reads showed excellent quality, with a mean Phred score higher than 30 for all base positions.