CantonS embryos, 6-8h afer egg laying, biological replicate #1
Small RNA in the 19-28nt range was isolated from total RNA by gel purification. Illumina TruSeq Small RNA Sample Prep Kit RA3 (3' adaptor) and RA5 (5' adaptor)  were sequentially ligated with gel purification after each ligation step.  cDNA was synthesized using the RTP primer and Superscript III and libraries were amplified using RP1 and RPI4.