GSM1520415: TS_EGFP; Mus musculus; RNA-Seq
Total RNA was prepared using TRI reagent (Sigma) followed by DNase treatment using the TURBO-DNA-free kit (Life Technologies), according to manufacturers' instructions. mRNA was isolated from total DNA-free RNA (150-240 ng) using the Dynabeads mRNA purification kit (Life Technologies 61006) and prepared into an indexed, strand-specific library using the ScriptSeq v2 RNA-Seq Library Preparation Kit (Epicentre SSV21106) according to manufacturers‘ instructions.  Libraries were quantified/assessed using both the KAPA Library Quantification Kit (KAPA Biosystems KK4824) and Bioanalyzer 2100 system (Agilent). Indexed libraries were pooled and sequenced with a 100bp single-end protocol.
GEO Accession
GSM1520415