GSM4065354: RPE, mm_16h_882R; Mus musculus; RNA-Seq
10 to 13-week-old male mice were randomly distributed over 6 time points (ZT0, 2, 4, 9, 14, 19). Each time, one mouse was sacrificed using cervical dislocation and eyes were collected via enucleation (n=5 mice per time point). Anterior segments (cornea, iris and lens) were removed to expose the posterior eyecup. The retina was carefully peeled off, and the eyecup containing the RPE, sclera and choroid were stored in RNAlater solution.   For RNA isolation, the RPE was removed gently from the choroidal layer using fine forceps. The brown clumps of RPE cells were dissolved in RLT Buffer from RNeasy Micro Kit after which RNA was extracted using the RNeasy Micro Kit. Illumina Next Seq 500 sequencer was used for 1 x 75 bp single-read sequencing, sequencing an average of 20 million reads per sample. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM4065354