tuf gene amplicons of positive control
tuf gene amplicons were prepared using primers TufFw: 5’-GGC CGT GTT GAA CGT GGT CAA ATC A-3’ and TufRv: 5’-TIA CCA TTT CAG TAC CTT CTG GTA A-3’ The PCR program included 94°C for 5 min and 30 cycles of 94°C for 30 s, 58°C for 45 s, 72°C for 90 s, and 72°C for 10 min (final extension).