Genome wide miRNA sequencing of SH-SY5Y cells overexpressing mutant MIR618 (clone 2)
SH-SY5Y cells stably overexpressing MIR618 with rs2682818 genotype CC (generated via lentiviral transduction). The paternal transduced cell line was split and clones were cultured separately to give biological replicates. Sequenced library: MIR618SNP cells, clone 2. Libraries were prepared using the TruSeq small RNA library kit (Illumina). PCR products were separated on a Pippin Prep system (Sage Science) to recover the 147 nt and 157 nt fractions containing mature miRNAs. Small RNA libraries were sequenced on a NextSeq 500 (Illumina) using single-end sequencing with read length of 75 bp. Library preparation and sequencing was performed at Biogazelle N.V. (Belgium).