RNA-seq of HEK 293T:  fluc_3
RNA extraction: Zymo Research Direct-zol RNA MiniPrep; library preparation: Illumina TruSeq Stranded Total RNA with Ribozero kit with two modifications: 1. RNA fragmentation at 94C for 2 minutes, and 2. following ligation, Beckman Coulter AMPure XP bead volumes reduced to 60% of suggested amount.