GSM2067513: Celf2 nuc CLIPseq; Mus musculus; OTHER
Nuclear fraction was incubated with NanoLink streptavidin magnetic beads for overnight at 4 °C followed by washes Samples were treated with micrococcal nuclease followed by phosphatase treatment, linker addition and 5’-labeling with 32P-γ-ATP. Complexes were eluted and separated with gel electrophoresis. A band corresponding to UNC-75/RNA complexes was cut out of the membrane and proteins degraded by Proteinase K. Associated RNAs were reverse-transcribed and amplified by PCR for deep sequencing.
GEO Accession
GSM2067513