GSM2064274: Parental cells (set 2); Homo sapiens; miRNA-Seq
10 µg of total RNA from parental and knockout cells was extracted with TRIzol reagent (Life Technologies). TruSeq Small RNA Sample Prep Kit (Illumina) was used for the preparation of the small RNA sequencing library. Total RNA was size-separated on a 15% urea-polyacrylamide gel. The region of the gel containing RNA with the size from 17 to 30 nucleotides was excised and eluted for adaptor ligation at both 5' and 3' ends. The ligated RNAs were reverse-transcribed with SuperScript III (Life Technologies) reverse transcriptase and amplified using Phusion High-Fidelity DNA polymerase (Thermo Scientific). The final products were sequenced by MiSeq system (Illumina).
GEO Accession
GSM2064274