GSM3855536: Graft sft//wt-rep2; Solanum lycopersicum; RNA-Seq
Five, seemingly homogeneous plants were selected from a larger population to represent the natural variation among greenhouse tomato plants. Independent stem samples consisted of 5 mid-segments of internodes harvested in duplicates 2 h after dawn. Total RNA was extracted using the RNeasy Mini Kit (Qiagen), and treated with DNase I using the RNase-Free DNase set (Qiagen) RNA samples were processed for sequencing, quality control, and differential expression analysis at the Technion Genome Center. Libraries of single 50 bp reads were prepared using the Ilumina TruSeq RNA library preparation kit v2. The barcoded libraries were sequenced on Ilumina Hiseq 2500, six samples per lane (~30,000,000 reads). If an experiment included more than 6 samples, each sample was divided equally, in all the lanes.
GEO Accession
GSM3855536