GSM3015259: fc_fetal_nuc64_3; Homo sapiens; RNA-Seq
Nuclear and cytosolic fractions were separated using protocol from Zaghlool A, Ameur A, Nyberg L, Halvardson J, Grabherr M, Cavelier L, Feuk L: Efficient cellular fractionation improves RNA sequencing analysis of mature and nascent transcripts from human tissues. BMC Biotechnol 2013, 13:99. Only cytoplasmic RNA was treated with the Ribo-Zero Magnetic Gold Kit for human/mouse/Rat (Epicentre) to remove ribosomal RNA, and purified using Agencourt RNAClean XP Kit (Beckman Coulter). The rRNA depleted cytoplasmic RNA and nuclear RNA. Sequencing libraries were prepared using the Ion Total RNA-Seq Kit for the AB Library Builder™ System and quantified using the Fragment analyzer (Advance Analytical) were then treated with RNaseIII according to Ion Total RNA-Seq protocol v2 and purified with Magnetic Bead Cleanup Module (Thermo Fisher).
GEO Accession
GSM3015259