GSM2752518: SETMAR(wt)_ChIPseq; Homo sapiens; ChIP-Seq
Cross-linked chromatin DNA was sonicated to an average length of 150 bp. Protein SETMAR-DNA complexes were immunoprecipitated using anti-FLAG M2 affinity agarose beads. After reversing crosslinks and digesting protein, ChIP DNA samples were purified by using a PCR purification kit (QIAGEN). ChIP DNA samples from SETMAR(wt) and SETMAR(R371A) groups were sent to the Center for Medical Genomics at Indiana University School of Medicine. The DNA libraries were prepared following to Illumina's instructions and sequenced on Illumina NextSeq500.
GEO Accession
GSM2752518