GSM1943680: SIN rep2; Caenorhabditis elegans; RNA-Seq
100 gravid adults were picked and washed in M9 for each sample. For OD1463 (WT) and OD1175 (SIN), worms homozygous for both transgenes and csr-1(tm892) were isolated from balanced heterozygous csr-1(tm892) mothers based on their differential motility. Total RNA was extracted using Trizol (Invitrogen, Carlsbad, CA, 15596-026) and was assessed for quality using an Agilent Tapestation; the RNA Integrity Number (RIN) for samples ranged from 7.9 to 8.9. RNA libraries were generated using Illumina’s TruSeq Stranded mRNA Sample Prep Kit using 500 ng of total RNA following manufacturer instructions. RNA libraries were multiplexed and sequenced with 50 basepair (bp) single end reads (SR50) to a depth of approximately 40 million reads per sample on an Illumina HiSeq2500 using V4 chemistry. All samples were run on the same lane.
GEO Accession
GSM1943680