Pc51265
Total RNA was extracted from the infection tissues using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer's protocol, then treated with DNase I (RNase free, TaKaRa, Japan) to remove genomic DNA contaminations. The quality, purity and concentration of each RNA sample were checked by using the 1% agarose gels, NanoDrop 2000c spectrophotometer (Thermo Scientific, Waltham, MA, USA) and Agilent 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Library preparation was performed from 1 µg of total RNA. Sequencing were conducted on Illumina HiSeq 2000 platform to produce100 bp paired-end reads (LC Company, Hangzhou, China)