GSM2407526: CD45RA- CD25hi eTreg Donor1; Homo sapiens; RNA-Seq
Total RNA was extracted from 1×106 cells using TRIzol reagent (15596-018, Ambion Life Technologies) according to the manufacturer’s protocol. The total RNA pellet was air-dried for 8 minutes and dissolved in an appropriate volume of nuclease free water (AM9937, Ambion Life Technologies) followed by a total RNA quantification using the Nanodrop UV-VIS Spectrophotometer (Thermo Scientific). The total RNA was further purified using the MinElute Cleanup Kit (74204, Qiagen) according to the manufacturer’s instructions. Quality and quantity of the total RNA was assessed by the 2100 Bioanalyzer using a Nano chip (Agilent, Santa Clara, CA). Total RNA samples having RNA Integrity Number (RIN)>8 were subjected to library generation RNA libraries were prepared for sequencing using standard Illumina protocols for stranded RNA sequencing on a hiseq2500 machine
GEO Accession
GSM2407526