Dasypyrum villosum Chromosome 4V short arm
Aqueous suspensions of chromosome 4VS of D. villosa were prepared from synchronized meristem root tip cells. DNA of the sorted chromosome arms was purified and then amplified by multiple displacement amplification (MDA) . The MDA-amplified DNA was used to create the two shotgun DNA-seq libraries of 500-700bp and 1000-1400bp inserted-size. The libraries were sequenced run in a single lane ofat Illumina HiSeq 2000 platform.