Illumina HiSeq 2000 sequencing; ChIP-seq of Tbx20 tagged with GFP in E11.5 mouse hearts
ChIP-seq of Tbx20 tagged with GFP in E11.5 mouse hearts
Embryonic Tbx20-GFP mouse hearts 11.5dpf were dissected and cross-linked in1.8% of formaldehyde for 10 minutes at room temperature. Samples were quenched by incubation with 2.5 M glycine for 5 minutes. Cells were  washed and resuspended in Lysis buffer (1% SDS, 10 mM EDTA, 50 mM  Tris, pH 8.1). The cross-linked chromatin was sonicated using a Misonix  4000 (amplitude 90 and 30 seconds on/30 seconds off) to an average size of  200 bp. Precleared chromatin extract was incubated overnight at 4AC with  goat anti-GFP and immunoprecipitated with protein AaSepharose beads.  10 ng of DNA was used to generate a standard Illumina sequencing library.  Enrichment of selected target regions was confirmed by qRT-PCR.  Illumina's standard protocol was used
Experimental Factor: none
immunoprecipitate