16s rRNA amplicon sequencing of a spring phytoplankton enrichment. Culturing temperature: fluctuating 16-24 C
Surface seawater from the San Pedro Ocean Time-series was enriched for phytoplankton and grown for two to three weeks under present-day or future temperatures that were either constant or fluctuated every 24 hours. DNA was extracted with Qiagens DNeasy Power Soil Kit and sequenced at MR DNA (Molecular Research LP) in Shallowater, Texas on an Illumina MiSeq with 2x300 reads. Targeted amplification of the 16s rRNA V4-V5 region was performed with primers from Parada et al. 2016: 515F-Y and 926R.