genome sequence of an H3N2 avian influenza
Extracted RNA was treated with DNase using Turbo DNA-free Kit (Ambion, Life Technologies) followed by rRNA depletion (Low Input Ribominus Kit, Life Technologies). The library was built using Ion Total RNA-Seq Kit (Life Technologies) before sequencing with the Ion Proton Sequencer and an Ion PI Chip v2 (Ion Torrent, Life Technologies).