GSM3931086: SS2_17_154_E11_R1; Mus musculus; RNA-Seq
Snap frozen tissue was dissociated with lysis buffer+0.32Msucrose in 1ml dounce homogenizers. Homogenate was added over a 1.8Msucrose layer and centrifuged 2 h 20 min at 30,000 × g (Beckman J-25 centrifuge with a J13.1 rotor).  cDNA was generated from FACS sorted single nuclei using Smart-seq2 protocol (Picelli et al. Nature Methods 2013).  ERCC spike-in RNA was added to the lysis buffer Nextera XT DNA sample preparation index kit, 384 indices (Illumina)
GEO Accession
GSM3931086