10dpp.ox.rep2
Library construction and deep sequencing was performed as described in {Herv* Seitz, Megha Ghildiyal, and Phillip D Zamore, “Argonaute loading improves the 5’ precision of both MicroRNAs and their miRNA* strands in flies,” Current Biology: CB 18, no. 2 (January 22, 2008): 147-151 AND Megha Ghildiyal et al., “Endogenous siRNAs derived from transposons and mRNAs in Drosophila somatic cells,” Science (New York, N.Y.) 320, no. 5879 (May 23, 2008): 1077-1081}. Size selection ranged from 18 to 35nt and the 2S rRNA depletion step was omitted.