GSM4065078: 1D_R2_IP; Apis mellifera; RIP-Seq
Total RNA from each sample was extracted using the RNA-extraction-system with TRIzol Reagent (15596018, Invitrogen). Polyadenylated RNA (polyA-RNA) were extracted using Dynabeads mRNA Purification Kit (61006, Invitrogen) Immunoprecipitated RNA fragments and comparable amounts of fragmented polyA-RNA (as input) were subjected to library construction with NEBNext Ultra RNA Library Prep Kit for Illumina (E7530S, New England Biolabs) according to the manufacturer's instructions.
GEO Accession
GSM4065078