miRNA seq of Centella asiatica CA308: Leaf (Rep.1)
Purified small RNA was ligated to 3' adapter and RT reaction was carried out to create single stranded cDNA. The cDNA is then PCR amplified. After libraries were constructed, they were sequenced on HiSeq 2500 with a 1x50bp reads to obtain around 15 million reads data. After the sequencing run, Illumina smallRNA-Seq data were processed to generate FASTQ files.