PROK RNA based amplicon 16S rRNA gene of a control SUP sample at day 157
cDNA sample obtained from RNA extract (MoBio PowerSoil RNA, High Capacity cDNA Reverse Transcription kit). Amplification of Hypervariable V4 region of prokaryotic 16SrRNA gene. 515F-806R primer set.  Dual-indexed Illumina compatible library in a single PCR step. Primary PCR with fused CS primersat their 5' ends. PCR products were normalized using Invitrogen SequalPrep DNA normalization plates.