Illumina MiSeq paired end sequencing; Genome sequencing of Phallusia mammillata
Genome sequencing of Phallusia mammillata
Sperm was collected from a single adult Phallusia mammillata animal obtained from the Roscoff Marine Station (France). Sperm from a single P. mammillata individual was lysed in 20 ml spooling buffer (75 mM NaCl, 25 mM EDTA pH 8.0, 1% SDS, 0.2mg/ml Proteinase K) at 55 degrees overnight with gentle shaking. 5 ml of saturated NaCl solution were added and the solution was mixed by gentle shaking till homogeneous. 25 ml of isopropanol were added and the solution was mixed by gentle shaking till homogeneous. The stringy DNA precipitate was fished out and washed in 70% ethanol. After removing the 70% ethanol, the DNA pellet was air dried and resuspended in 10mM Tris pH 8 at 55 degrees with gentle shaking. A genomic DNA library was prepared using  the NEBNext Ultra II DNA library preparation kit starting with 50 ng of sheared material following the manufacturer's instructions.
Experimental Factor: organism
Phallusia mammillata