GSM2065367: Control for FTO knockdown 2; Homo sapiens; RNA-Seq
For RNA-Seq, total RNA was collected using the TRIzol reagent, and was DNase-treated prior to library construction. For miCLIP, total RNA was collected using the TRIzol reagent and treated with DNase. Then, poly(A)+ RNA was selected using oligo(dT) magnetic beads prior to subjecting the samples to the miCLIP protocol. For RNA-Seq, libraries were prepared using the Illumina TruSeq Stranded mRNA Library Prep Kit. For miCLIP, libraries were constructed using the iCLIP cloning process, as previously described (see GSE63753).
GEO Accession
GSM2065367