Illumina HiSeq 1000 paired end sequencing; Promoter capture GM12878 CD34
Promoter capture GM12878 CD34
GM12878 cells were obtained from the Coriell Cell Repositories and grown in suspension in RPMI 1640 medium, supplemented with 2 mM glutamine, 15% FBS and 1% penicillin/streptomycin under standard culture conditions. The white blood cell population was col Hi-C was performed following (Lieberman-Aiden et al., Science, 2009, DOI: 10.1126/science.1181369 ) with modifications detailed in (Mifsud et al.). For CHiC experiments promoter interactions were enriched using a biotynilated RNA bait library as described
Experimental Factor: cell type
B cell