GSM4497100: 40L; Gallus gallus; RNA-Seq
Total RNA was extracted from each individual otocysts using the RNAqueous™-Micro Total RNA Isolation Kit (Ambion) according to the manufacturers protocol. The quality of isolated RNA was tested using Agilent 2200 Tapestation and only samples with RIN of at least 9 were used for library Libraries were made using SMART-Seq v4 Ultra Low Input RNA Kit (Clontech): cDNA was generated using the SMART (Switching Mechanism at 5' End of RNA Template) technology during 10 cycles of PCR. cDNA integrity and quantity was checked on the Agilent Bioanalyser using the High Sensitivity DNA kit, and 200pg of cDNA was then converted to sequencing library using the Nextera XT DNA protocol (Illumina Samples were sequenced 43bp paired-end read and high output - 400 million reads per run on NextSeq 500 instrument (Illumina)
GEO Accession
GSM4497100