Genome sequencing and assembly of Klebsiella pneumoniae
Genomic DNA was isolated from cultures grown in Lysogeny Broth overnight. DNA was extracted in 96-well format from 100 uL of sample using the MagAttract PowerMicrobiome DNA/RNA Kit (Qiagen, Hilden, Germany) automated on a Hamilton Microlab STAR robotic platform.  Bead disruption was conducted on a TissueLyser II (20 Hz for 20 min) instrument in a 96 deep well plate in the presence of 200 uL phenol/chloroform.  Genomic DNA was eluted in 90 ul water after magnetic bead cleanup. The resulting genomic DNA was quantified by Pico Green.