Illumina HiSeq 2500 sequencing; RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project.
RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project.
Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik ,  Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426
Experimental Factor: Sik1 knockout
genotype
Experimental Factor: adipose tissue
organism part
Experimental Factor: gonad
sampling site