GSM4065466: ∆lsd1 N7983 bisulfite-seq; Neurospora crassa; Bisulfite-Seq
Cultures were grown in Vogel's medium and 1.5% sucrose (standard minimal medium), shaking for 18 hours at 32˚C. Genomic DNA was isolated using a protocol modified from (Pomraning et al, 2009). Genomic DNA (1µg) was treated with bisulfite and libraries were prepared for sequencing using the NEBNext Ultra DNA Library Prep Kit for Illumina (E7370; NEB) and EpiMark Bisulfite Conversion Kit (E3318; NEB). The library was purified with Agencourt AMPure XP beads (Beckman Coulter), quantified using a Qubit HS Assay (Life Technologies), visualized on the Fragment AnalyzerTM (Advanced Analytical), and sequenced on a Illumina HiSeq 4000
GEO Accession
GSM4065466