V1V2 16S rDNA PCR, amplicon purification, measuring amount of                     amplicon DNA, amplicon sequencing on 454 GS FLX
V1V2 primers
A+V1_For:GCCTCCCTCGCGCCATCAGAGAGTTTGATCMTGGCTCAG ;                          B+V2_Rev:GCCTTGCCAGCCCGCTCAGCYNACTGCTGCCTCCCGTAG