GSM3856159: LH3; Sus scrofa; miRNA-Seq
Total RNA was isolated individually from the ovaries samples using TRIzol Reagent (Invitrogen), purified with RNeasy Mini Kit (Qiagen). Total RNA of each sample was quantified and qualified by Agilent 2100 Bio-analyzer (Agilent Technologies, Palo Alto, CA, USA), NanoDrop (Thermo Fisher Scientific Inc.) and 1% agrose gel. Illumina small RNA-Seq (RNA sequencing) libraries were constructed using NEBNext® Multiplex Small RNA library Prep Set for Illumina® (NEB, USA) following manufacturer's recommendations.
GEO Accession
GSM3856159