GSM1622583: ANME-1 Mcr-producing M. acetivorans grown on methane, Replicate 2; Methanosarcina acetivorans; RNA-Seq
Rapid centrifugation in the presence of 50 µL RNAlater solution (Ambion, Austin, TX) per mL of culture, followed by flash-freezing of cell pellets. Total RNA was isolated using RNeasy Mini kit (Qiagen, Valencia, CA), and were further digested with terminator 5’-phosphate-dependent exonuclease (Epicentre, Madison, WI) to partially remove ribosomal RNA. Digested RNA were cleaned up using AgenCourt RNAClean XP beads (AgenCourt Bioscience, Beverly, MA). RNA libraries were prepared for sequencing using standard Illumina protocols (TruSeq Stranded mRNA Library kit).
GEO Accession
GSM1622583