DNA-Seq of Galdieria sulphuraria RT22: Microevolution at 28C - 67 Generations after start
DNA was extracted using the Genomic-tip 20/G column (QIAGEN, Germany) following the "Yeast" DNA extraction protocol. DNA size and quality was assessed via gel electrophoresis and Nanodrop (Thermo Fisher, USA). TruSeq DNA PCR-Free libraries (insert size = 350 bp) were generated. The samples were quantified using the KAPA library quantification kit and quality controlled using the Bioanalyzer (Agilent). The samples were sequenced on a Illumna HiSeq 3000 in paired end mode (1x150bp) at the Genomics and Transcriptomics Laboratory of the Biologisch-Medizinisches Forschungszentrum in Dsseldorf, Germany.