GSM4498301: NURF301BAP170_RNAseq_Rep2; Drosophila melanogaster; RNA-Seq
For 3'RNA-seq, we counted and aliquoted 10e6 cells and added a fixed amount of ERCC polyadenylated spike-in RNA. We then isolated total RNA using TRIzol, followed by ethanol precipitation. RNA was removed using RNase-free DNase I. Libraries were constructed from 325 ng total RNA using the QuantSeq 3' mRNA-seq Library Prep Kit (Lexogen) with the UMI add-on kit per manufacturer instructions.
GEO Accession
GSM4498301