GSM2408695: CH-S6; Mus musculus; RNA-Seq
Eight week C57BL6 c-KitCreERT2/mTeG mice were injected for 14 consecutive days with tamoxifen (0.5 mg/day by i.p) to induce Cre expression and to label c-Kit(+) cells with eGFP. Mice underwent MI and two days later were treated with vehicle or Sfrp2. C-Kit(+) cells were isolated one day prior to MI, one day post-MI and 12 days post-MI (vehicle and Sfrp2). Cardiac biopsies were isolated for c-Kit(+) cells. Total RNA from the purified c-Kit(+) population was isolated using a PicoPure Arcturus kit (Invitrogen). Complimentary cDNA was generated with an Ovation Pico WTA System V2 kit (NuGEN).
GEO Accession
GSM2408695