Genome sequencing Botrylloides leachii
DNA was sheared to 300-400 bp using a Covaris S220 sonicator before the addition of the 120 bp Illumina adapter sequence, and agarose size selection of 400-500 bp fragments. The average fragment  size used( for( was 450bp. The library was made using the Illumina TruSeq DNA v2 library preparation kit.