GSM3993089: E11.5 TrkCcre;Tom; Mus musculus; RNA-Seq
Brachial DRGs were dissected and collected in Leibovitz L-15 medium (Life technologies) on ice. Then the DRGs were incubated in 0.05% trypsin-EDTA (1x) (Life technologies) for 5, 7, and 10 mins for DRGs from E9.5, E10.5 and E11.5, respectively, at 37 °C, shaking at 700 RPM. After spinning down the samples at 100 RCF for 5 mins, the supernatant was removed and replaced by Leibovitz L-15 medium (Life technologies). DRGs were physically triturated using 2 different sizes of pipettes previously coated with 0.2% bovine serum albumin until the solution homogenized. The cell suspension was then filtered through 70 ?m cell strainer (BD Biosciences) to remove clusters of cells. RNA libraries were prepared according to Smart-seq2 protocol.
GEO Accession
GSM3993089