GSM2752191: bisulfite sequencing by TEPBAT sperm; Mus musculus; Bisulfite-Seq
High molecular weight genomic DNA was prepared from tail and sperm samples of the same adult mouse by a standard procedure. The DNAs were treated with 10M bisulfite, and their comprelemtary strands were synthesized by Klenow fragment with tagged-random primer (5'-GCAGTGAACTGACTACAGGNNNN-3'). IAP-containing genomic regions were amplified by PCR using KOD-plus neo, tag primer (5'-CAGTGAAC- TGACTACAGG-3'), and either of IAP-BS1 (5'-GGGGAAGGTAGAGTATAWG-3') or IAP-BS2 (5'-GGTTTTTGAAGATGTAAGTAATAAAGTTTT-3') primer. The PCR products of 350 to 450 bp were purified from a 2% agarose gel, and the Illumina sequencing adaptors were added to their ends using TruSeq DNA Sample Prep kit (Illumina). BS-seq (target enrichment after PBAT)
GEO Accession
GSM2752191