siRNA knockdown of CWC22 - biological replicate 1
Total RNA was extracted with Isol-RNA lysis reagent (5 Prime) and 1 μg of RNA was used as input material for library preparation. Ribosomal depletion and strand specific library preparation was carried out with the TruSeq® Stranded Total RNA LT - (with Ribo-ZeroTM Human/Mouse/Rat) according to the manufacturer’s instructions.