RNAseq of Guar
Purification and fragmentation of mRNA from 1ug of total RNA followed by first stand and second strand cDNA synthesis. Next, end repairing of cDNA was performed followed by 3’end adenylation and adaptor ligation. The cDNA libraries were then enriched by PCR amplification. Validation of the libraries was performed on using Agilent DNA 1000 kit on the Agilent 2100 bioanalyzer. Normalization and pooling of libraries and finally libraries were sequenced on Illumina MiSeq.