GSM3552971: wt_spleen_2; Mus musculus; RNA-Seq
Tissues were harvested immediately after euthanasia, snap frozen in liquid nitrogen and stored at -80˚C. Tissues were subsequently pulverized in liquid nitrogen and homogenized in TRIzol. Embryonic Mouse Fibroblasts (MEF, passage 2) and mouse embryonic stem cells (ESC) were cultured in 10cm dishes in normal conditions (37˚C, 5% CO2) and homogenized directly in Trizol. Standard illumina protocols were used to make libraries (TruSeq RNA Sample Preparation Kit v2)
GEO Accession
GSM3552971