GSM3856552: N2_24h_IFN_smallRNA; Myotis daubentonii; miRNA-Seq
The medium was removed and the cells washed once in PBS. Total RNA were extracted using the RNeasy kit (Qiagen) according to the manufacturer's instructions. The samples from each treatment were pooled and the amount of RNA determined using a Nanodrop. The quality and integrity of the respective RNA were deterimend using a Agilent 2100 Bioanalyzer. The samples were then stored at -80°C RNA libraries were prepared for sequencing using the Illumina TruSeq smallRNA Kit
GEO Accession
GSM3856552