GSM4337082: Nhe1 Astro-KO Contralateral [300_CL]; Mus musculus; RNA-Seq
Single cell suspension of astrocytes from the ipsilateral and contralateral hemispheres from wild-type and Nhe1 Astro-KO brains after 24 h tMCAO (transient middle cerebral artery occlusion) were prepared using Adult Brain Dissociation Kit (Miltenyi Biotec, Germany) following manufacturers protocol. RNA from ACSA-2+ astrocytes was extracted on the QIAsymphony SP (Qiagen Corporation, Germany) using the QIAsymphony RNA Kit RNA libraries were prepared for sequencing using Illumina HiSeq 2500
GEO Accession
GSM4337082