RNA-Seq of Alouatta caraya: adult female skin
Total RNA extraction from skin transcriptome was achieved using TRIzol Reagent (Life Technologies, Cat. 15596-026) according to the manufacturers protocol. RNA samples were further purified using RNeasy Mini Kit (Quiagen, Cat. 74104) and treated with DNaseI (Quiagen, Cat. 74254), according to the manufacturers protocol. RNA (0.5 g) was reverse transcribed using the SureSelect Strand-Specific RNA Library Prep for Illumina Multiplexed Sequencing (Agilent), In order to establish a skin transcriptome reference, a HiSeq Illumina run was obtained from skin library with 150 bp paired-end reads