454 16S rRNA amplicon sequence of North Atlantic bacteria: Sample AFH58
Ten liters of seawater were filtered onto 0.2-µm poresize filters (47 mm diameter), flash frozen in liquid nitrogen and stored at -80ºC. DNA was extracted using the UltraClean Soil DNA Isolation Kit (MoBio).The amplification of the V1–V3 region of the 16S rRNA gene was performed by Research and Testing Lab- oratory (Lubbock, TX) with bacterial primers BAC28F (GAGTTTGATCNTGGCTCAG) and BAC519R  (GTNTTACNGCGGCKGCTG), followed by pyrosequencing by using a Roche 454 GS-FLX system with titanium chemistry.