AB SOLiD 4 System sequencing; Transcription profiling by RNA-Seq mouse embryos
Transcription profiling by RNA-Seq mouse embryos
Palatal shelves from 5 embryos were dissected from E13.5 mouse embryos and pooled for RNA extraction. The same was done for E14.5 mouse embryos. All mice were housed with free access to food and water under specific pathogen free conditions at the University of Manchester. Temperature 20-22 °C, Humidity 40-60%, Specified mouse pathogen free conditions, 12 hours light/dark cycle. Nutrients Rat and Mouse Standard Diet no.1 expanded (Ban Kingman). All experiments were performed under regulations of The UK Home Office Scientific Procedures Act (1986). BALB/c mouse strain (Charles River Ltd, UK) were used for the experiment. Embryos were obtained after matings, the morning of the vaginal plug being considered embryonic day (E) 0.5. RNA was isolated mouse palatal shelves using the Qiagen RNeasy kit. SOLiDTM Total RNA-Seq Kit (PN 4445374)
Experimental Factor: developmental stage
embryonic day 13.5
LIBRARY_STRAND
first strand