Pooled sample of Illumina 16S rRNA gene amplicon sequences from water and substrate from coal combustion (fly ash) contaminated and reference sites used in Hughey et al. AEM 2016
Illumina MiSeq sequencing of the V4 region of the 16S rRNA gene using primers 515F and barcoded 806R. DNA template was DNA sample extracted from water and substrate obtained from coal combustion (fly ash) contaminated and reference sites using the Qiagen Dneasy Kit.