GSM4560305: XBP1s_ChIP_CT12; Mus musculus; ChIP-Seq
The comprehensive CT0 and CT8 DNA libraries were prepared at the MD Anderson Cancer Center, which generated ~40 million 75 bp paired-end reads per samples. The CT0 to CT36 time point DNA libraries were prepared at the University of Houston Sequencing Core and generated ~30 million 75 bp single-end reads per sample. DNA libraries generated from 1ng input DNA were prepared with KAPA Hyper Prep Kit (KAPABIOSYSTEMS) for the comprehensive CT0 and CT8 DNA libraries and with Ovation® Ultralow V2 DNA-Seq library preparation kit (NuGen) for the CT0 to CT36 time point DNA libraries. The size selection was performed using SPRIselect beads (Beckman Coulter), followed by quality control analyzed using the High Sensitivity DNA chip on Bioanalyzer 2100 (Agilent). The DNA libraries were pooled and sequenced using NextSeq 500 (Illumina).
GEO Accession
GSM4560305