rdr2
Custom libraries for ~15-80 nt RNAs were constructed and sequenced by Fasteris SA (http://www.fasteris.com/). In brief, 5 μg total RNA was subjected to 15% denaturing polyacrylamide gel electrophoresis. The desired RNA size-range was excised and eluted, and 3’ and 5’ adapters were added according to the ligation-based Illumina small RNA-seq protocol. Sequencing by synthesis (1x100) was performed on an Illumina HiSeq 2500 instrument following manufacturer’s recommendations (TruSeq SBS Kit v3). Basecalling was performed using the HiSeq Control Software (v2.0.10.0), RTA (v1.17.21.3) and CASAVA (v1.8.2).