GSM3929479: Whole seedlings, GFP-FLAG.CHIP.rep2; Arabidopsis thaliana; ChIP-Seq
Three gram of bZIP68-FLAG or GFP-FLAG seedling per sample was cross-linked with 1% formaldehyde for 10 min, and then incubated with 0.125 M glycine (final concentration) for an extra 5 min. Chromatin extraction and immunoprecipitation was carried out according to the protocol from Joseph Ecker lab (Song et al., 2016). DNA-Protein complexes were pulled down using FLAG antibody (Sigma Aldrich, F1804) which has been bound to Pierce™ ChIP-grade Protein A/G Magnetic Beads (ThermoFisher, 26162). The immune complexes were treated with NaCl, RNase A, proteinase K and 65 degree heating for de-crosslinking. Finally, DNAs were purified using ChIP DNA Clean & Concentrator™ kit (ZYMO research, D5205). Illumina sequencing libraries preparation was carried out according to the manufacturer's manual (CWBIO, CW2585S, NGS Fast DNA Library Prep Set for Illumina). The libraries were sent to Novogene company (Tianjin, China) and sequenced using the Illumina HiSEQ1500 platform.
GEO Accession
GSM3929479