Genome sequence of Mitsuaria sp. TWR114
Genomic DNA was extracted using NucleoSpin Microbial DNA Kit and NucleoSpin Bead Type B (Macherey-Nagel, Dren, Germany) and ultrasonicated by a Bioruptor (Cosmo Bio Co., Ltd., Tokyo, Japan). Barcoded libraries were prepared using the Ion Xpress Plus gDNA Fragment Library Preparation kit (Thermo Fisher Scientific, Waltham, MA, USA). Library size selection was performed by using the E-Gel system and Size Select 2% Agarose Gels (Thermo Fisher). After amplification and purification of the libraries, DNA concentration was determined by the Qubit 2 Fluorometer system (Thermo Fisher). Diluted libraries were amplified through emulsion PCR on the Ion OneTouch 2 instrument (Thermo Fisher) with the Ion PGM Hi-Q View OT2 kit- 400 (Thermo Fisher). The PCR products were enriched on the Ion OneTouch Enrichment System (Thermo Fisher). Sequencing was performed on the Ion PGM Sequencer with the Ion PGM Sequencing Hi-Q View Sequencing Kit (Thermo Fisher) and the Ion 318 Chip v2 (Thermo Fisher).