GSM4065877: cc003_14_non; Homo sapiens; RNA-Seq
For RNA-seq, the invasive and noninvasive cells were harvested separately by trypsin digestion. scRNA-seq libraries were prepared per the Single Cell 30Reagent Kit UserGuide v2 (10x Genomics Chromium Single cell 3' RNA v2). Cellular suspensions were loaded on a ChromiumController instrument (10x Genomics) to generate single-cell gel bead-in-emulsions (GEMs). GEM-reverse transcriptions (GEM-RTs) were performedin a Veriti 96-well thermal cycler (Thermo Fisher Scientific). After RT, GEMs were harvested and the cDNAs were amplified and cleaned up with theSPRIselect Reagent Kit (Beckman Coulter). Indexed sequencing librarieswere constructed using the Chromium Single-Cell 30Library Kit (10x Geno-mics) for enzymatic fragmentation, end-repair, A-tailing, adaptor ligation, liga-tion cleanup, sample index PCR, and PCR cleanup. The barcoded sequencinglibraries were quantified by quantitative PCR using the KAPA LibraryQuantification Kit (KAPA Biosystems).
GEO Accession
GSM4065877