RNAseq of Res259 low grade glioma lineage
For library construction, 300 ng of  high-quality RNA and the TruSeq Stranded Total RNA LT Sample Prep Kit  (Illumina Inc) were applied. Firstly, the RiboZero technology (Illumina Inc.)  was used to remove rRNA and preserve only poly(A) and other non-poly(A)  transcripts, which were then fragmented. RNA fragments sized between 200500  bp were utilized to generate the sequencing libraries. Clustering was  performed in an automated system cBot (Illumina Inc) and samples were  sequenced with TruSeq SBS kit v5, single-read 72 cycles.