Illumina HiSeq 2000 sequencing; Modified iCLIP sequencing of HEK293 cells with antibody PTBP1
Modified iCLIP sequencing of HEK293 cells with antibody PTBP1
HEK293 cells were crosslinked with 0.15mJ/cm2 254nm UV light. Following the iCLIP protocol the retrieval of protein-bound RNAs and construction of Illumina compatible libraries was done as described in Huppertz et al, 2014. The 3 prime adapter sequence is AGATCGGAAG
Experimental Factor: replicate 3
individual
Experimental Factor: antibody PTBP1
immunoprecipitate