GSM2065869: HET10 +CMC; Mus musculus; OTHER
Total spleens of C57BL/6J mice were harvested, and total RNA was isolated using the RNeasy Mini Kit following manufacturer’s protocol (Qiagen) Pseduo-seq libraries were prepared as described (Carlile et al. Nature 2014) Briefly, total RNA was fragmented, and was subsequently derivatized with CMC followed by reversal of CMC adducts. Fragments were size selected, followed by 3′ adapter ligation, and reverse transcription. Truncated RT fragents were size selected, circularized, and PCR amplified. PCR products were gel purified, and sequenced on an Illumina HiSeq 2000. OTHER: Pseudo-seq
GEO Accession
GSM2065869