Illumina sequencing of pectoral fin RNA from Protopterus dolloi
Right pectoral and pelvic fins of lungfish (Protopterus dolloi) were used for the total RNA extraction separately. The RNAs were extracted with TRIzol reagent (Life Technologies, Inc.) according to the manufacturer's procedure. After the DNase I treatment, magnetic beads with Oligo (dT) were used to isolate mRNA. The mRNA samples were separately used for the cDNA synthesis and construction of paired-end library with ~200 bp inserts. The cDNA libraries were used for sequencing using Illumina HiSeq 2000 at BGI (Shenzhen, China).