GSM4338094: D4_Nor_10X; Homo sapiens; RNA-Seq
The single cell suspension was washed twice with 1x PBS + 0.04% BSA.   The cell number and concentration was confirmed with TC20™ Automated Cell Counter  Approximately Number (5000, etc.) cells were subjected immediately onto the 10XGenomics Chromium Controller machine for Gel Beads-in-Emulsion (GEM) generation.  mRNA was prepared using 10x Genomics Chromium Single Cell 3' reagent kit (V2 chemistry).  During this step, cells were partitioned into the GEMs along with Gel Beads coated with oligos. These oligos provide poly-dT sequences to capture mRNAs released after cell lysis inside the droplets, as well as cell-specific and transcript-specific barcodes (16 bp 10X Barcode and 10 bp Unique Molecular Identifier (UMI), respectively).   After RT-PCR, cDNA was recovered, purified and amplified to generate sufficient quantities for library preparation. RNA libraries were prepared for sequencing using standard 10x Genomics protocols.Library quality and concentration were assessed using Agilent Bioanalyzer 2100.  Libraries were run on the Hiseq X for Illumina PE150 sequencing.
GEO Accession
GSM4338094