RNA-Seq of Cannabis sativa:20d developing seed
1)mRNA enrichment and purification: rRNA depletion or Oligo dT Selection to enrich the mRNA // 2)RNA fragment and reverse transcription // 3)End repair, add A and adaptor ligation // 4)RCR amplification // 5)Single strand separation and cyclization // 6)DNA nanoball synthesis // 7)Sequencing on BGISEQ platform