small RNA-seq of O. rufipogon: seedlings
Small RNA were gel purified from total RNAs,  and then the 5 and 3 adapters were ligated to the small RNA molecules. The  ligated RNA were proceeded to be reversely transcribed and amplified by PCR.  Finally, six small RNA libraries were constructed and sequenced on Hiseq2500  analyzer at BerryGenomics in Beijing, China (http:// berrygenomics.com).