GSM4183287: Gar1513_17005L; Homo sapiens; RNA-Seq
Total RNA was extracted from breast cancer cell lines and tissue samples using RNeasy Mini Kit (Qiagen) following the manufacturer's instructions. For the tumour tissue, snap frozen sections were first disrupted in lysis buffer in a TissueLyser II (QIAGEN). The next generation sequencing data was processed on the Illumina HiSeq 2500 v4.0 System. Sequences were trimmed using Trim Galore (version 0.4.4) and genome-guided alignment to a human reference (HG38) was performed using the STAR software (version 2.5). Feature counts were computed in R (version 3.4.3) using the package Rsubread.
GEO Accession
GSM4183287