RNA-Seq of  Aspergillus fumigatus A1163: GPCRs involved in cell wall damage
Total RNA was  extracted using Trizol (Invitrogen), treated with RQ1 RNase-free DNase I  (Promega) and purified using a RNAeasy Kit (Qiagen) according to  manufacturer's instructions. RNA from each treatment was quantified using a  Qubit fluorometer and analyzed using an Agilent 2100 Bioanalyzer system to  assess the integrity of the RNA.  Illumina TruSeq Stranded mRNA Sample Preparation kit was used to  construct cDNA libraries following manufacturer's instructions.