GSM1945261: T4.Control_11; Mus musculus; RNA-Seq
RLT lysis, SPRI purification, Turbo DNase, MaximaRT, Random hexamer 2nd strand cDNA 50% of total first strand cDNA, 1ng of ds_cDNA per 96-well plate, 12 minutes and run for 16 cycles PCR according to Nextera manufactures protocol Modified SCRB-Seq, Soumillon, M., Cacchiarelli, D., Semrau, S., Oudenaarden, A. van & Mikkelsen, T. S. Characterization of directed differentiation by high-throughput single-cell RNA-Seq. bioRxiv 003236 (2014). doi:10.1101/003236; Modified Nextera protocol to PCR enrich only  the 3' end of mRNA transcripts and create digital gene expression libraries for Illumina sequencing.
GEO Accession
GSM1945261