RADseq for Diatraea saccharalis: sample5
A Restriction site Associated DNA tag (RADtag) paired-end library was constructed for SNP and microsatellite discovery. RADseq paired-sequencing data were analysed using a local read aligenment pipeline without reference genome, for SNP discovery and genotyping. Filtered raw reads were de novo assembled for microsatellite discovery and annotation. The goal of this study was discovey molecular markers in a non-model species to study its ecological adaptation to host-plants and pest management.