GSM4560964: J2_gGII.4_24h; Homo sapiens; RNA-Seq
RNA were extracted from 5-day differentiated, mock-inoculated monolayers at 3 hpi, and from GII.4- or gGII.4-inoculated monolayers at 6, 10 and 24 hpi using Qiagen RNeasy mini Kit (Qiagen). The RNA integrity was assessed on a 2100 Bioanalyzer (Agilent Santa Clara, CA). Paired-end sequencing (100 bp) was performed on an IlluminaHiSeq 2500. Sequencing was performed with ribosomal RNA depletion, 100-bp paired reads (2X100) with 30 million paired end reads for a total of 60 million reads per RNA sample.
GEO Accession
GSM4560964