Swine fecal phage metagenome from nonmedicated animals 17 days after start of the study
Phages were isolated from pooled feces from 6 pigs using a cesium-chloride gradient followed by DNase treatment, phage head lysis,and DNA isolation.  Individual preparations of phage DNA were quantified using Quant-iT PicoGreen (Invitrogen, Carlsbad, CA) and used to deliver 500 ng of DNA into the Rapid Library Preparation method (454 Life Sciences, Branford,CT).  Libraries were created with Roche multiplex identifier (MID) labeled adaptors.  Individual libraries were pooled into one of two groups in an equimolar fashion.  Pooled preparations were used to prepare DNA beads for sequencing using a two-region picotiter plate on a Roche GS-FLX instrument using Titanium chemistry (454 Life Sciences).  MID forward ACGCGTCTAGT  MID reverse complement ACTAGAGGCGT  