GSM4561515: EPC_niPSC_PXGL_MEF_rep1; Homo sapiens; RNA-Seq
Total RNA was extracted from two biological replicate cultures of each cell line and time point using TRIzol/chloroform (Thermo Fisher Scientific, 15596018), and RNA integrity assessed by Qubit measurement and RNA.Ribosomal RNA was depleted from 1 µg of total RNA using Ribozero (Illumina kit). nanochip Bioanalyzer. Sequencing libraries were prepared using the TruSeq RNA Sample Prep Kit (RS-122-2001, Illumina). Sequencing was performed on the Novaseq S1 or S2 platform (Illumina), according to the manufacturer's instructions.
GEO Accession
GSM4561515