16S rRNA Illumina MiSeq  results from DNA extracted from produced water, Niobrara shale,  Denver-Julesburg Basin
DNA  extractions, amplifications, and Illumina MiSeq 16S rRNA gene sequencing were  completed by Argonne National Laboratory in Lemont, IL. The MoBio PowerSoil  htp-96 well Isolation Kit (MoBio Laboratories, Carlsbad, CA) was used for DNA  extractions following the manufacturers given protocol. Following  extraction, the V4 region of the 16S rRNA gene was amplified using the 515F  (5  GTGTGYCAGCMGCCGCGGTAA  3) and 806R primer pair, and PCR was performed  using the Promega PCR Master Mix (Promega catalog #: M5133, Madison WI)  according to the manufacturers protocol. The amplicons were sequenced on a  151 bp x 12 bp x 151 bp llumina MiSeq (San Diego, CA) run.