GSM3553095: ChIP-Seq_HCC1806_H3K27ac; Homo sapiens; ChIP-Seq
HCC1806 and HCC1937 cells were cross-linked with 1 % formaldehyde (for H3K27Ac), or cross-linked with disuccinimidyl glutarate, followed by double cross-linked with 1 % formaldehyde (for BRD4). The libraries were constructed following NEB's Chip-Seq Sample prep kit. Briefly, Chip DNA was end-blunted and added with an 'A' base so the adaptors from Illumina with a 'T' can ligate on the ends. Then 200–400 bp fragments are gel-isolated and purified. The library was amplified by 18 cycles of PCR.
GEO Accession
GSM3553095