HGADFN164.late_passage
The library preparation was performed as per the manufacturer's protocol (NimbleGen SeqCap EZ). Sheared DNA samples were subjected to three enzymatic steps: end repair, A-tailing, and ligation to Solexa paired-end indexed adapters. The DNA libraries were indexed,  were PCR-amplified, pooled, and in-solution hybridized to a custom NimbleGen SeqCap EZ Choice Library (Roche, Inc.) of complementary oligonucleotide DNA baits. Following washing, the captured libraries were amplified by PCR and were subjected to sequencing. 