GSM2644930: VIS-EPA-9; Mus musculus; miRNA-Seq
At the end of 11 week feeding period, the mice were fasted for 4 hours and then euthanized by CO2 inhalation. Epidedimal (VAT), inguinal (SAT) and interscapular (BAT) adipose tissues were harvested, and snap frozen immediately in liquid N2, followed by storage at -80°C. Frozen adipose tissue samples (SAT, BAT, and VAT- 3 samples per group) were homogenized in QIAzol® (QIAGEN, Redwood City, CA).  Total RNA was isolated according to manufacturer's protocol using RNeasy Mini Kit. Small RNA libraries were prepared using the Illumina small RNA protocol (Illumina, Inc., San Diego, CA)
GEO Accession
GSM2644930