Small RNA sequencing of schistosoma japonicum 22 dpi female worm
22 dpi female S. japonicum total RNA was isolated using QiagenmiRNeasy Mini Kit (Valencia, CA) according to the manufacturers instruction.  10 g total RNA was separated by electrophoresis on a Novex 15% TBE-Urea gel (Invitrogen Co. Ltd) and RNA in length of 18-30 nt was purified and ligated to 3 and 5 adapters. Then for reverse transcription, we used a RT-PCR kit (Invitrogen Co. Ltd) to produce complementary DNA (cDNA). Finally, the cDNA was amplified using adapter primers, the products were purified using a 6% TBE PAGE gel (Invitrogen Co. Ltd) and solexa sequencing.