GSM3854450: F2: Female rep2; Rattus norvegicus; RNA-Seq
Anterior pituitaries were quickly collected from postpubertal rats and dispersed using a trypsin/EDTA based method. Four pituitaries per sex were collected, pooled, dispersed and counted, then two samples per sex were loaded into a total of four lanes of the Chromium Single Cell Controller (10X Genomics) Single-cell cDNAs and barcoded sequencing libraries were constructed from the output of the Chromium Single Cell Controller using a Chromium Single Cell 3' Reagent Kit v2 according to the manufacturer's protocol (10X Genomics) Each library received a different sample index; these were multiplexed and sequenced together. Barcodes = cell barcodes
GEO Accession
GSM3854450