GSM4330938: Empty pIC Rep2; Mus musculus; RNA-Seq
The cells were thawed by placing the plates/tubes on ice. Total RNA was isolated using RNeasy plus kit (Qiagen) according to vendor recommended protocol. RNA concentration was estimated by nanodrop (Thermo) 2 ug of total RNA was used to isolate mRNA through magnetic beads using mRNA isolation kit (PolyA mRNA isolation Module, NEB) followed by RNA-seq library preparation using mRNA library preparation kit (NEB) strictly following the vendor recommended protoco.l
GEO Accession
GSM4330938