Tc517_454
500ng of genomic DNA was used to generate a 454 sequencing library using the Roche 454 Rapid Library kit. Sequencing beads were made in four large volume emulsion PCR reactions targeting no more that 15% of the beads amplifying to reduce losses at polyclonal filtering and sequenced on 1.5 titanium picotiter plates, which generated 801Mb of data with an average read-length of 329bp. This sequence was assembled into 5751 large contigs with an N50 contig size of 12.6kb and a sum of 31Kb by Newbler version 2.5.3.