GSM2064545: MDA MB-231 cell line control replicate 2; Homo sapiens; RNA-Seq
After treatment medium is removed and a total RNA isolation kit from Exiqon was used to extract both mRNA and miRNA. The kit itseld includes the binding of RNA (mRNA and miRNA) to a spin column, followed by a series of washes for purification and finally elution. RNA libraries were prepared for sequencing using standard Illumina protocols.  The Truseq Stranded mRNA kit was used to prepare mRNA libraries from 1ug total RNA.  Libraries were confirmed on the Agilent 2100 Bioanalyzer and quantitated using the Illumina Library Quantification Kit, ABI Prism qPCR Mix from Kapa Biosystems and the ABI7900HT real-time PCR instrument.  76 cycle single read sequencing was performed with the 500 High-output v2 (75cycle) sequencing kit on the Illumina NextSeq500 instrument.
GEO Accession
GSM2064545