iPSC RNA: Sample 142e5ecb-d354-4b16-9d4e-ee0110fd07d8
Total RNA was isolated using the Qiagen RNAeasy Mini Kit from frozen RTL plus pellets, including on-column DNAse treatment step. RNA was eluted in 60 µl RNAse-free water and run on a Bioanalyzer (Agilent) to determine integrity. Concentration was measured by Nanodrop. Illumina Truseq Stranded mRNA libraries were prepared and sequenced on HiSeq2500 or HiSeq4000, to an average of 40 M 100 bp paired-end reads per sample. RNA-Seq reads were aligned using STAR60 with a splice junction database built from the Gencode v19 gene annotation.
alignment_software
STAR