Sexually Active Male Gonad of Brevoortia tyrannus
The dUTP-strand specific cDNA library was made using chemical hydrolysis and the Illumina Ultra Directional RNA-seq kit (NEB). Libraries were barcoded with Tru-Seq adaptors and amplified with 12-15 cycles of PCR (Illumina). Completed RNAseq libraries were quantified with Qubit DNA HS, BioAnalyzer High Sensitivity DNA (Invitrogen, Agilent, KAPA). The libraries were sequenced using the NextSeq 500 High Output 300 cycles kit, reading 155 x 155 bp Paired End Sequencing.