Paired-end reads of Warea amplexifolia
An Illumina paired-end shotgun library was prepared by shearing 1 ug of DNA (Illumina TruSeq DNA Library Kit, San Diego, CA) and using a multiplex identifier adapter index. DNA was sheared using a Covaris S220 (Woburn, MA). Using a Hi Seq 2000 (Illumina), sequencing with 100-bp paired-end reads was performed. PAL_FINDER_v0.02.03 (Castoe et al., 2012) was used to examine five million reads and to extract reads containing microsatellite repeats.