GSM2486367: MPC_S2_SPOPwt; Mus musculus; RNA-Seq
RNA extraction of cell pellets as well as frozen cores from fresh frozen tissue was done as described by manufacture using the automated Maxwell®16 system (Promega, AS1280 and AS1270). cDNA synthesis was transcribed by using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, #4374966). Quantitative PCR was done on the Roche Light Cycler 480. Organoids (5 pairs from model A and one pair from model B of SPOP mutant and controls) were prepared for RNA sequencing using TruSeq RNA Library Preparation Kit v2. RNA integrity was verified using the Agilent Bioanalyzer 2100 (Agilent Technologies). Each sample was then sequenced with the HiSeq 2500 to generate 2×51-bp paired-end reads.
GEO Accession
GSM2486367