Poly-A Selected metatranscriptomic sequencing from 502 wild-caught adult Drosophila obscura
Our objective was to identify virus-like sequences associated with Drosophila obscura in the wild.  502 Adult male flies were collected (280 flies collected July 2011 Edinburgh 55.928N, 3.170W; 52 flies October 2011 Edinburgh 55.928N, 3.170W; 115 flies July 2011 Sussex 51.100N, 0.164E; 55 flies August 2011 Perthshire 56.316N, 3.790W). RNA was extracted using Trizol, and submitted (dry, in RNAstable) to BGI for poly-A selection and paired-end RNA sequencing.