GSM3930373: BJAB cells, Infected with EBV, biological rep 1, m6A-RIP; Homo sapiens; RIP-Seq
Intact mRNA was isolated from total RNA samples using Arraystar Seq-StarTM poly(A) mRNA Isolation Kit according to the manufacturer's protocol. Isolated mRNA was chemically fragmented into 100-nucleotide-long fragments by incubation in fragmentation buffer. m6A methylated mRNAs were immunoprecipitated with anti-N6-methyadenosine (m6A) antibody, and also a part of the fragmented mRNAs was kept as input. The major procedures contained immunoprecipitation, wash and elution. Both m6A enriched mRNAs and input mRNAs were concentrated for RNA-seq library construction. RNA-seq libraries of m6A antibody enriched mRNAs and input mRNAs were prepared using KAPA Stranded mRNA-seq Kit (Illumina).
GEO Accession
GSM3930373