GSM4065191: Anthers, SAND_CONTROL_rep3; Oryza sativa; RNA-Seq
Spikelets from the middle part of the panicles were collected, dissected to separate the anthers and collected in tubes suspended in liquid nitrogen. Total RNA was extracted from pooled anthers by using TRIzol® (Invitrogen). Three biological replicates were collected for each treatment (Cold-stress and Control). The quality and quantity of RNA samples were assessed by gel electrophoresis and Nanodrop quantification. RNA libraries were prepared for sequencing by using Truseq mRNA kit at IGA Technology Services (Udine, Italy).
GEO Accession
GSM4065191