RNA-Seq of  Aspergillus fumigatus A1163: GPCRs involved in cell wall damage
Total RNA was extracted using Trizol  (Invitrogen), treated with RQ1 RNase-free DNase I (Promega) and purified  using a RNAeasy Kit (Qiagen) according to manufacturer's instructions. RNA  from each treatment was quantified using a Qubit fluorometer and analyzed  using an Agilent 2100 Bioanalyzer system to assess the integrity of the  RNA. Illumina TruSeq Stranded mRNA  Sample Preparation kit was used to construct cDNA libraries following  manufacturer's instructions.