GSM2066575: ago4-1;PAGO4-FM-AGO3; Arabidopsis thaliana; Bisulfite-Seq
The plants were collected in liquid nitrogen, ground with morthat and pestle, and DNA extracted in CTAB, Rnase treated and purified with phenol:chloroform followed by Ethanol precipitation. Bisulfite conversion with EpiTect Kit (Qiagen); Approximately 1 ug of genomic DNA were used to generate libraries using premethylated adapters (NEXTFlex Bisulfite-Seq Adapters, Bioo Scientific #511911). The adaptor-ligated fragments were purified by QIAQuick column (QIAGEN) and bisulfite converted using the EpiTect Kit (Qiagen) following the manufacturer’s instructions. The converted DNA was later enriched by 15 cycles of PCR using the Pfu Turbo Cx Polymerase (Agilent), using the specific primers provided by Bios Scientific for enrichment. The library was finally purified with Agencourt AMPure XP beads (Beckman Coulter) according to manufacturer’s instruction.
GEO Accession
GSM2066575