Shotgun sequencing of Monachus monachus: female skin
Double-stranded DNA Illumina libraries were built from the extracts. A blunt-end library was constructed on 21.25 L of the DNA extract using NEBNext DNA Sample Prep Master Mix Set 2 (New England Biolabs, E6070). The protocols outlined in the kit manual with some modifications (Raghavan et al., 2014; Rey-Iglesia et al., 2020).