Illumina MiSeq sequencing; qiita_ptid_4493:2248.BLANK14
The purpose of the present study is to gain initial insight into the role of alterations to the gut microbiome in the development of age-related physiological dysfunction in a setting of lifelong Western diet consumption, and to determine whether healthy lifestyle or dietary interventions that improve physiological function may be effective in part due to preservation or improvement in the composition of the gut microbiome.
Illumina EMP protocol 515f, 806bcr amplification of 16S rRNA V4
barcode
AAGACAGCTATC
center_name
CU Boulder
center_project_name
seals aging microbiome mice
experiment_center
CCME
illumina_technology
MiSeq
linker
GT
miseq_runid
140701_M00517_0195_000000000-A7H2B
pcr_primers
FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
physical_specimen_remaining
false
primer
GT
run_center
CCME
run_date
7/1/14
runid
140701_M00517_0195_000000000-A7H2B
samp_size
.1,g
sample_center
CCME
sample_type
XXQIITAXX
sequencing_meth
Sequencing by synthesis
study_center
CCME
target_gene
16S rRNA
target_subfragment
V4