GSM1701995: H3K4me3_Hypoxia; Homo sapiens; ChIP-Seq
The cells were lysed by radioimmunoprecipitation assay (RIPA) buffer and the cell lysates were collected by centrifugation. Immunoprecipitated DNA was isolated with selected antibodies. Libraries were prepared according to Illumina's instructions. Briefly, 10 ng of DNA was end-repaired using a combination of Klenow polymerase and T4 polynucleotide kinase to yield a protruding 3-d'A' overhang. After adapter ligation, DNA was PCR amplified with Illumina primers for 18 cycles and library fragments of 100~500 bp were band isolated from an agarose gel. The purified DNA was captured on agilent bioanalyzer. Libraries were sequencedHiSeq 2000 platform following the manufacturer's protocols.
GEO Accession
GSM1701995