GSM3611750: ID00016 Hi-C; Homo sapiens; Hi-C
Lymphocytes were isolated from whole blood samples by centrifugation after dilution with PBS and underlaying Sigma Histopaque-1077 to the bottom. Genomic DNA and total RNA were extracted simultaneously from lymphocytes using QIAGEN's AllPrep DNA/RNA Mini Kit. Five million cells were crosslinked with formaldehyde and lysed with protease inhibitors (Sigma, P8340). Chromatins were subsequently digested by MboI restriction enzyme (NEB, R0147). Restriction fragment overhangs were filled and the DNA ends were marked with biotin. Next, biotinylated DNA were sheared to a size of 300-500 bp, whose ends were repaired subsequently. dATP were attached and Illumina indexed adapter (TrueSeq nano) were ligated. The DNA fragments were then amplified and purified.
GEO Accession
GSM3611750