GSM4066037: CD4 T-cell H3K27me3 lowArg Input; Homo sapiens; ChIP-Seq
Cells were washed with PBS and fixed in either 1% FA for 10 minutes (histones) or 2mM DSG for 30 minutes and 1% FA for 30 minutes (transcription factors). Cells were sonicated to give fragments of 100-300bp followed by IP. DNA libraries were made using the NEBnext ultra DNA library preparation kit for Illumina (Cat no. E7370).
GEO Accession
GSM4066037