GSM3929500: Islets Donor 21; Homo sapiens; RNA-Seq
Aliquots of cultured human islets and their respective pseudoislets were used for RNA extraction using NucleoSpin® RNA XS (Macherey-Nagel, Düren, Germany). RNA integrity (RIN≥9) was measured with Bioanalyzer 2100 (Agilent Technologies) mRNA was isolated from 120 ng RNA by poly-dT enrichment using the NEBNext Poly(A) mRNA Magnetic Isolation Module. After fragmentation, the samples were subjected to the workflow for strand-specific RNA-Seq library preparation (Ultra Directional RNA Library Prep II, NEB) and 75bp single read sequencing was performed on Illumina NextSeq500.