16S rRNA amplicon sequencing of human stool sample from vaginally delivered, antibiotic nave, exclusively breastfed infant at approx 1 month of age
The V1-V2 region of the 16S rRNA gene was amplified by using primers 8F, 338R, and BifidoF. 25 amplification cycles were employed. Sequencing was performed on the MiSeq platform (Illumina).