Illumina HiSeq 4000 sequencing; 10x_v2 sequencing
unspecified
Process - Process Core - Process Id
lib32
Library Preparation Protocol - Protocol Core - Protocol Id
library_preparation_protocol_1
Library Preparation Protocol - Protocol Core - Protocol Name
Library preparation for 10x_v2 sequencing
Library Preparation Protocol - Protocol Core - Protocol Description
ChromiumTM Single Cell 3' Library & Gel Bead Kit v2
Library Preparation Protocol - Protocol Core - Document
10x_sequencing_protocol.pdf
Library Preparation Protocol - Cell Barcode - Barcode Read
Read 1
Library Preparation Protocol - Cell Barcode - Barcode Offset
0
Library Preparation Protocol - Cell Barcode - Barcode Length
16
Library Preparation Protocol - Input Nucleic Acid Molecule
polyA RNA
Library Preparation Protocol - Library Construction Method
10X 3' v2 sequencing
Library Preparation Protocol - Nucleic Acid Source
single cell
Library Preparation Protocol - End Bias
3 prime tag
Library Preparation Protocol - Primer
poly-dT
Library Preparation Protocol - Strand
first
Library Preparation Protocol - Umi Barcode - Barcode Read
Read 1
Library Preparation Protocol - Umi Barcode - Barcode Offset
16
Library Preparation Protocol - Umi Barcode - Barcode Length
10
Sequencing Protocol - Protocol Core - Protocol Id
sequencing_protocol_1
Sequencing Protocol - Protocol Core - Document
10x_sequencing_protocol.pdf
Sequencing Protocol - Method
tag based single cell RNA sequencing
Sequencing Protocol - Instrument Manufacturer Model
Illumina HiSeq 4000
Sequencing Protocol - Paired End
false
Sequencing Protocol - 10X - Fastq Method
samtools fastq
Sequencing Protocol - 10X - Fastq Method Version
1.9-48-g2d4907c
Sequencing Protocol - 10X - Pooled Channels
4.0
Sequencing Protocol - 10X - Drop Uniformity
true
Input Biomaterial - Biomaterial Core - Biomaterial Id
A21-Spl-1-SC-1_cells
Input Biomaterial - Biomaterial Core - Biomaterial Name
Fresh spleen cells
Input Biomaterial - Biomaterial Core - Biomaterial Description
Fresh spleen cells
Input Biomaterial - Biomaterial Core - Ncbi Taxon Id - 0
9606
Input Biomaterial - Selected Cell Types - 0
spleen immune cells
Input Biomaterial - Genus Species - 0
Homo sapiens
Input Biomaterial - Estimated Cell Count
3092
Input Biomaterial - Cell Morphology - Percent Cell Viability
94.0
Input Biomaterial - Cell Morphology - Cell Viability Method
Trypan blue; manual haemacytometer (C-chip)
HCA Process UUID
278141e1-8e7e-4eed-94fd-c5c774378d92
HCA Library Preparation Protocol UUID
6f399c41-797f-4f69-8719-cbd468478e68
HCA Sequencing Protocol UUID
09b0afde-d355-4ebf-b9ba-8eee36d1a5e3
HCA Input Biomaterial UUID
897d7c7e-dd8d-424f-a421-49ab03e6dba7