GSM2406917: A549_H3K9ac_Control_B; Homo sapiens; ChIP-Seq
The ChIP was performed with 15 min (for KLF10) and 10 min (for histone modifications) crosslinking in 1 % formaldehyde in PBS. Following chromatin shearing , 1µg antibody was used for immunoprecipitation overnight. The immunocomplexes were incubated 2 additional hours with protein A sepharose beads and subjected to washes. After reversing the cross-linking, the DNA was isolated by standard phenol-chloroform-isoamyl extraction procedure. ChIP-Seq libraries were prepared with MicroPlex Library Preparation Kit following the manufacturer's instructions.
GEO Accession
GSM2406917