GSM3854178: HACAT_treated_rep2; Homo sapiens; RNA-Seq
RNA was isolated using the Direct-zol RNA mini Prep kit (Zymo Research). Therefore a Trizol-Lysat (Invitrogen) of 2 mio harvested cells of each condition was used as input. Isolated RNA was quantified and quality controlled by using a NanoDrop and Bioanalyzer respectivly. Before sequencing library generation, 1 µg of isolated RNA was additionally purified using Agencourt RNAClean XP beads. Following quantification by NanoDrop and quality control by Bioanalyzer, 500 ng high quality RNA (RIN > 7,5) were used to generate sequencing libraries using the SENSE mRNA-Seq Lib Prep Kit V2 (Lexogen). Libraries consist only of polyadenylated transcripts.
GEO Accession
GSM3854178