GSM3855868: Salmonella_exposed_ind2; Homo sapiens; RNA-Seq
4 hours after infection, the cells were washed with PBS, resuspended in 120ul of 5mM EDTA and incubated 5min in RT to detach cells that might have attached the dish. The EDTA was then washed and the cells were counted with trypan blue, suspended with 0.04% BSA in PBS and directly used for single-cell sequencing by the Chromium Single Cell 3' Reagent version 2 kit and Chromium Controller (10X Genomics, CA, USA) as previously described at Zheng, G. X. Y. et al Nature Communications 2017. Libraries were prepared by the Chromium Single Cell 3' Reagent version 2 kit and Chromium Controller (10X Genomics, CA, USA) as previously described at Zheng, G. X. Y. et al Nature communications 2017.