GSM3544380: Sample_105_R19L_RNA; Mus musculus; RNA-Seq
L4 and L5 DRGs were diaassociated by enzymatic treatment, and single sensory neurons were manually isolated. Libraries were constructed using scMT-seq following our previous published protocol. scMT-seq consists of three steps: isolation of nucleus cytoplasma, single cell RNA-seq using cytoplasma and single cell whole genome bisulfate sequencing using the nucleus. Briefly, cell membrane specific lysis buffer were used to lyse membrane. Cell nucleus were picked out and put into nucleus lysis buffer. The remaining cell plasma were used for transcriptomic profiling by SMART-seq2. For the single cell whole genome bisulfate sequencing, the library was constructed by performing the single cell whole genome bisulfate sequencing protocol on the isolated nucleus. Both RNA-seq and WGBS libraries were sequenced on a Hiseq 4000 machine in pair-end, 150bp mode. scMT-seq, low-input RNA-seq and low input whole genome bisulfate seqeuncing.
GEO Accession
GSM3544380