GSM3929880: K3; Mus musculus; RNA-Seq
Total RNA was extracted from purified sperm using the miRNeasy Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. The RNA samples were concentrated and purified by an RNA-bead cleanup with Agencourt® RNAClean® XP Beads (Beckman Coulter, Krefeld, Germany). 2 ng of isolated RNA were treated with DNaseI (Thermo Scientific). Afterwards, the RNA was used to generate double-stranded cDNA using the Ovation RNA Seq V2 Kit (NuGEN) according to the manufacturer's instructions. Sequencing libraries were generated using the Ovation Rapid DR Multiplex System (NuGEN) according to the manufacturer's instructions. 250 ng of fragmented and purified cDNA were used to prepare the libraries.
GEO Accession
GSM3929880