Danio Rerio 7 dpf GLDC+/+
Library preparation was performed using the Truseq RNA (Illumina). 13 PCR cycles were required to amplify cDNA libraries. Libraries were quantified by Nanodrop and BioAnalyzer. All libraries were diluted to 10 nM and normalized with the Miseq SR50 v2. Libraries were pooled to equimolar concentration and multiplexed by 6 samples per lane.