[Illumina] of [Rhopilema esculentum]:[natural population]
A total of 3 ug total RNA from each sample was prepared for transcriptome sequencing. Sequencing libraries were constructed using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) and barcode index was added to distinguish the sequences from each sample. Three microliter of USER Enzyme (NEB, USA) was used for size selection. The daptor-ligated cDNAs were held at 37 oC for 15 min and at 95 oC for 5 min respectively before amplification. The PCR products were purified before sequencing, and quality control of the library was conducted on an AMPure XP system coupled to an Agilent Bioanalyzer 2100 system.