GSM1623248: NEJmir; Fasciola hepatica; miRNA-Seq
Samples were homogenized using the TissuLyser II (QIAgen) and the RNA extraction was performed with the RNeasy Mini Kit (QIAgen) following the manufacturer's instructions. The amount and integrity of total RNA was determined using a 2100 BioAnalyzer (Agilent). 3 μg of total RNA was size-fractionated on a 15% TBE urea polyacrylamide gel and the 15-30 base-pair fraction was excised. The RNA was retrotranscribed into single-stranded cDNA using the Superscript II reverse transcriptase (Invitrogen) and the Illumina’s small RNA RT-Primer following the manufacturer’s instructions. The cDNA was PCR amplified in 15 cycles using Illumina’s small RNA primer set. The PCR products were quantified and subjected to single end Illumina sequencing for 75 cycles.
GEO Accession
GSM1623248