16S rRNA V4 microbiota of channel catfish: Gut_USDA503_5
The entire intestinal tract and its contents were removed using sterile procedures, then homgenized using a sterile chilled mortar/pestle, and a PowerFecal DNA isolation kit was used to isolate DNA. The Fluidigm Access Array PCR platform was used to generate 16S rRNA V4 amplicon library using 515f and 806r PCR primers, indexing barcodes, and Illumina sequencing primers. Library was sequenced on an Illumina MiSeq using v3 chemistry.