GSM1623054: Osam1 mutant RNA-seq; Oryza sativa Indica Group; RNA-Seq
Total RNA from young panicles in meiosis was prepared from both wild-type and Osam1 mutant plants using Trizol according to the manufacture’s protocol (Invitrogen, http://www.invitrogen.com). An OligoTex mRNA mini kit (Qiagen) was used to isolate poly (A) mRNA and to prepare a nondirectional Illumina RNA-Seq library with an mRNA-Seq 8 Sample Prep Kit (Illumina). The gel extraction step was modified by dissolving excised gel slices at room temperature to avoid underrepresentation of AT-rich sequences. Purified DNA libraries were amplified by 18 cycles of PCR. The products were loaded onto an Illumina HiSeq2000 instrument and subjected to 100 cycles of paired-end (2×100 bp) sequencing.
GEO Accession
GSM1623054