plasmids of Sinorhizobium meliloti isolated strains
Genomic DNA of alfalfa rhizobia was isolated from overnight cultures using the Wizard Genomic DNA Purification Kit (#A1120, Promega, USA). Genome sequencing was performed at Unidad de Genmica, Instituto de Agrobiotecnologa y Biologa Molecular, CICVyA, INTA (https://inta.gob.ar). A total of 12.87 million (M) paired-end reads of 32- or 101-bp read length were obtained from Illumina Hiseq1500 technology, resulting in an average of 34-fold genome coverage. De novo assembly of psyn plasmids were performed using Geneious assembler platform (www.geneious.com).