Illumina HiSeq 2000 paired end sequencing; ChIP-seq analyses of CTCF binding in liver samples from CAST/EiJ and SPRET/EiJ (Mus spretus) mice, and of the cohesin subunit RAD21 in C57BL/6J mouse liver
ChIP-seq analyses of CTCF binding in liver samples from CAST/EiJ and SPRET/EiJ (Mus spretus) mice, and of the cohesin subunit RAD21 in C57BL/6J mouse liver
Liver samples were collected from three Mus m. castaneus and three M. spretus individuals Liver tissue from three male individuals from the Mus species was crosslinked, dounced and lysed as described in Schmidt et al. 2009 (PMID: 19275939). The chromatin was sonicated using a Misonix sonicator 3000 homogenizer with 12 cycles of 30s ON and 60s OFF at a power-output 30 Watt. CTCF was immunoprecipitated using the Merck-Millipore antibody (cat# 07-729). NGS libraries prepared according to Schmidt et al. 2009 (PMID: 19275939) ligating Illuminas low-plex TruSeq adapters.
Experimental Factor: immunoprecipitate
input DNA
Experimental Factor: organism
Mus musculus castaneus