L octovalvis, shutgon seq
50 ng genomic DNA was fragmented using a in-house at the University of Missouri-Columbia (Columbia, MO, USA) Q800R2 Sonicator (Qsonica Newtown, CT, USA). Libraries were prepared and indexed using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) and the NEBNext Multiplex Oligos for Illumina (paired end) (New England Biolabs, Ipswich, MA, USA) following the manufacturer specification. Indexed DNA was sequenced on an Illumina Miseq using 2 x 150 bp paired-end reads.