Lachancea waltii ARS Library
L. waltii genomic DNA obtained from logarithmically growing cultures was randomly sheared by sonication.  Sheared DNA was end-repaired and run through a gel to size select 350 and 1,000 base pair fragments.  Fragments were cloned into a yeast centromeric plasmid that otherwise lacks an origin of replication.  The library of plasmids was transformed into L. waltii using selection for the plasmids.  Plasmids were recovered from yeast colonies able to grow on the selective medium.  Plasmid inserts were amplified from the plasmid by PCR using primers containing Illumina sequencing adapters.