GSM2409884: EWS; Homo sapiens; OTHER
The 3' linker labelled by gama-32P-ATP was ligated to RNA-protein complex before SDS-PAGE. After nitrocellulose transfer, the band above EWS was excised and treated with Proteinase K, and the 5' linker was ligated. To isolated RNA, NaOAc, glycogen, 1:1 EtOH:isopropanol was added and precipitated overnight at -20°. Linker-ligated RNAs were reverse transcribed with Superscript III (Life Technology) and PCR-amplified with Phusion polymerase (ThermoFisher).  The product was fractionated on 4% agarose gel and recovered DNA was quantified for deep sequencing CLIP-seq
GEO Accession
GSM2409884