GSM3930391: KO rep5; Mus musculus; RNA-Seq
Total RNA was harvested using Rneasy Plus Mini Kit (74136, Qiagen). PolyA-tail specific mRNA libraries were prepared from 1 ug of total RNA with the Illumina TruSeq stranded mRNA library preparation protocol, followed by single-end 1x75bp sequencing with the Illumina NextSeq.