Cadmium-treated group, bioreplication 2
DNA library preparation is completed by terminal repair, addition of A tail, addition of sequencing linker, purification, PCR amplification using the NEBNext Ultra DNA Library Prep Kit for Illumina kit (New England BioLabs, UK). The constructed library was PE sequenced by the Illumina Hiseq 2500 platform (Illumina, San Diego, CA).