Illumina HiSeq 2000 sequencing; qiita_ptid_73:632.Temperate.deciduous.forest
In an effort to enable the sharing of genetic material from environmental samples for the benefit of the scientific community, we are establishing the Canadian MetaMicroBiome Library. This repository will house composite soil samples that are characterized by extensive 16S rRNA gene and whole-genome sequencing, generating taxonomic profiles and metabolic pathway reconstructions for each sample. The sequence data will be submitted to existing public databases and linked with detailed physical, chemical and geographic characterization of the samples according to established metagenomic standards. Sample DNA will be used to construct high quality libraries in cosmid vectors, allowing for phenotypic screening in a broad range of microbial surrogate hosts. Phenotypic screening of metagenomic libraries provides access to truly novel functions that would otherwise be missed by sequence-based surveys of bulk community DNA or metagenomic libraries. MetaMicroBiome is being initiated with soil samples collected from across Canada, and initial libraries are being screened for novel glycoside hydrolase activities in multiple surrogate hosts. Once MetaMicroBiome is established, contributions of samples from other environments will be accepted for inclusion. The resulting resource will provide the international scientific community with access to a common collection of thoroughly characterized community-contributed genetic material in clone library format for custom phenotypic screen
EMP V4 515f,806rbc protocol
barcode
CGTAAGATGCCT
center_name
U Waterloo
center_project_name
Canadian MetaMicroBiome Initiative
emp_status
EMP
illumina_technology
HiSeq
linker
GT
pcr_primers
FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
primer
GTGTGCCAGCMGCCGCGGTAA
run_center
CCME
run_date
9/1/11
run_prefix
s_3_1_sequences
samp_size
.1,g
sample_center
LBNL
sequencing_meth
sequencing by synthesis
study_center
CCME
target_gene
16S rRNA
target_subfragment
V4