Illumina HiSeq 2500 sequencing; Rabbit RNA-seq time-series of the development of seven major organs.
Rabbit RNA-seq time-series of the development of seven major organs.
The organs were dissected from 15 developmental stages (starting at e12 until P548). Until e14 the dissected 'brain' samples correspond to the whole brain, and from e15.5 onwards they correspond to the cerebral hemispheres (without the olfactory bulbs). Early 'cerebellum' samples correspond to the prepontine hindbrain-enriched brain region (until ~ e19.5) and from then onwards only to the cerebellum. RNA was extracted using the RNeasy protocol from QIAGEN. RNeasy Micro columns were used to extract RNA from small (< 5 mg) or fibrous samples and RNeasy Mini columns were used to extract RNA from larger samples. The tissues were homogenized in RLT buffer supplemented with 40 mM dithiothreitol (DTT) or QIAzol. The RNA-seq libraries were created using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina).
Experimental Factor: organism part
heart
Experimental Factor: developmental stage
embryonic day 16.5
Experimental Factor: sex
female