GSM2482921: nrpd1a-4 (Ler) x nrpd1a-4 (Col) rep 1; Arabidopsis thaliana; RNA-Seq
Seeds from crosses between multiple individuals were hand-dissected and total embryo or endosperm RNA isolated using the Ambion RNAqueous Micro Kit. DNase I-treated RNA (Invitrogen, Life Technologies Corporation, Carlsbad, CA) was used to prepare mRNA-seq libraries using the Truseq Stranded mRNA Library Prep Kit as directed (Illumina Inc., San Diego, CA). Amplification was for 15 cycles. Libraries were sequenced with 100x100 base paired end reads on an Illumina HiSeq 2000 machine.
GEO Accession
GSM2482921