GSM3990116: 5.LE-1We-m2-S5-all-R1-001; Bos taurus; RNA-Seq
Total RNA from the fat tissue of three cows per group was extracted using a RNeasy Midi kit (Qiagen®, Courtaboeuf, France) and purified using a DNAfree kit (Invitrogen by Life Technologies), according to the manufacturer's recommendations. Directional RNA-Seq libraries were constructed using the TruSeq Stranded Total RNA library prep kit (Illumina), following the manufacturer's instructions, using 750 ng of total RNA.