Whole genome sequencing of Saccharomyces cerevisiae SY46-1
Yeast strain SY46 expressing APOBEC3B were subcultured (from a single cell to a colony) on YPD (containing hygromycin) plates for ten generations. A single colony from the tenth generation was purified on a normal YPD plate. A purified colony was incubated on a normal YPD plate to obtain enough cells for genomic DNA extraction and whole genome sequencing by Illumina technology.