GSM3612745: RNA-seq_WT pre-B rep2; Mus musculus; RNA-Seq
Bone marrow B220+ cells were purified using anti-CD45R coated MACS beads (Miltenyi Biotec) following manufacturer's instruction. Pre-pro B cell (B220+AA4.1+IgM-CD43+CD24-) and pre-B cell (B220+AA4.1+IgM-CD43-CD24+) were isolated by flow cytometry. Total RNA was extracted by TRIzol reagent (Thermo Fisher Scientific), followed by DNA digestion by RNA Clean & Concentrator-5 (ZYMO RESEARCH), and mRNA was obtained by poly (A) selection with mRNA DIRECT kit (Thermo Fisher Scientific). RNA-seq libraries were prepared using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) following manufacturer's protocol. Libraries were prepared using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs)
GEO Accession
GSM3612745