GSM1706624: DAT1; Brassica napus; RNA-Seq
Based on cytological observation, we knew that the key stage respond to gametocide amidosulfron was young floral buds before uninucleate microspore. Therefor, young floral buds from amidosulfuron-treated (DAT) and control plants (F) were dissected, put into RNAlater and stored at −80°C for later use. Total RNAs were extracted using the Trizol® Reagent (Invitrogen, USA) and purified using TRK1001 Kit (LC Science, Houston, TX). The total RNA quantity and purity were analyed by using of Bioanalyzer 2100 with RNA 6000 Nano LabChip Kit (Agilent, CA, USA). We used 10 µg total RNA from each sample to create normalized cDNAs. Three independent biological replicates were used for the herbicide or control treatment. RNA libraries were prepared for sequencing using Illumina’s Digital Gene Expression Tag Profiling Kit according to the manufacturer’s protocol
GEO Accession
GSM1706624