GSM2671097: T47D-sh-Hectd1-1 (n=1); Homo sapiens; RNA-Seq
Total RNAs form confluent T47D cell lines infected with shNT, HectD1-shRNAs (#1 and #2) or transfected with HA-ACF7 for 72 hrs, were extracted using the RNeasy kit (Qiagen). RNA integrity was assessed on a Bioanalyzer RNA pico chip. The RNA-seq libraries were generated with mRNA enrich Kapa stranded kit and sequencing PE50 was performed on a HiSeq 2500. Reads were aligned to the GRCh37 genome with Star v2.5.0, and the raw alignment counts were calculated with featureCounts v1.5.0 .
GEO Accession
GSM2671097