metatranscriptomics of human vagina: vaginal swab sample from pregnant woman: Sample EP580086_K40_MV1R
Whole shotgun metatranscriptomic sequencing: The rRNA depleted messenger RNA was prepared for sequencing by constructing cDNA libraries using the KAPA Biosystems KAPA RNA HyperPrep Kit. Indexed cDNA libraries were pooled in equimolar amounts and sequenced on the Illumina HiSeq 4000 instrument running 4 multiplexed samples per lane.