WGS of R. communis: leaf
Libraries were generated using the sample preparation kit of Illumina as per the manufacturers protocol. Fragmentation of genomic DNA (1 g) was carried out using Covaris S2 System followed by adapter ligation. Enrichment of adapter-modified DNA fragments by PCR was performed. Paired-End cluster generation kit (Illumina Inc., USA) was used for cluster generation on paired-end flow cells. Cluster generation was carried out by hybridization of WGS libraries with a concentration ranging from 9-55 nmol/L, which were further diluted to make a stock solution of 4 nmol molecules onto the oligonucleotide-coated surface of the flow cell. Isothermal amplification of the libraries was carried out to generate clonal DNA clusters.