GSM3856224: G5.GAL76.SD12hr.56537; Mus musculus; RNA-Seq
After cervical dislocation the brains were quickly removed and 3mm coronal section containing VLPO region was cut and post-fixed in ice-cold 4% paraformaldehyde (pH 7.4) for 5 minutes. 10 μm frozen sections were generated with cryostat and GFP+ cells were isolated using LCM. Total RNA was extracted using RNAqueous-Micro kit (Invitrogen). Total RNA from each sample was prepared for sequencing using theTakara Bio, SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian. 1 ng of RNA was used to prepare the RNA libraries. The libraries were sequenced on Illumina HiSeq 4000. Depths of 23-36 million paired-end 150bp reads were generated for each sample.
GEO Accession
GSM3856224