GSM3855641: Cysts, 4hr_3; Rhodospirillum centenum; RNA-Seq
Cells homogenized in Trizol immedietly after collection. Stored at -80C till extraction. Extracted using Zymo Directzol RNA kit Library prepared at Univerisity of Wisconsin, Madison - Biotechnology center. Briefly, ribosomal RNA from total RNA removed using Epicentre Ribo-Zero Magnetic (Bacteria) kit with input quantity of 1.5 microgram total RNA. TruSeq RNA sample prep kit (Illumina, San Diego, CA) was used to prepare cDNA mRNA-seq libraries. Standard illumina protocol used for sinle-end sequencing
GEO Accession
GSM3855641