GSM1620055: CeM projector, experiment-2: CeM_546; Mus musculus; RNA-Seq
Total RNA was isolated using Arcturus PicoPure RNA isolation kit (Life Technologies). Double stranded cDNA was generated from total RNA using a DNA-RNA chimeric primer mix and reverse transcriptase, and cDNA was amplified by the SPIA amplification process developed by NuGEN (Ovation RNA-Seq System V2 kit (Neugen)). The amplified double stranded cDNA was fragmented to an average size of 200 bases using Covaris S-S series sonication system. The fragmented cDNA was end-repaired, and adaptors and barcodes were ligated before PCR-based library preparation using Ovation Ultralow DRMultiplex Systems (Nugen).
GEO Accession
GSM1620055