Oxaliplatin-resistant HCT116 transfected with siRNA targeting circCCDC66
Total RNA from oxaliplatin-resistant HCT116 cells transfected with siRNA control oligonucleotides or ones against backsplice junction of circCCDC66 for 24 hours were isolated using TRI reagent (Sigma-Aldrich) according to the manufacturers instructions and quantified by using Agilent 2100 Bioanalyzer (Agilent Technologies). The ribosomal RNA was depleted by using Ribo-zero rRNA Removal Kit (Epicentre), and subsequent library was constructed by using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB). The RNA-seq was performed on the Novaseq 6000 platform (paired-end 2 x 150 bp).