GSM3856445: RNAseq in POMC_ACreb3l2 Tg IL replicate 2; Mus musculus; RNA-Seq
NIL from WT or Tg mice were dissected and snapfreezed up to RNA extraction. Total RNA from pools of 5 NILs was extracted using the Qiagen RNeasy+ miniprep kit (including a genomic DNA removal step). Ribosomal depletion was performed on the extracted RNA. Libraries were prepared according to Illumina's instructions. Libraries were sequenced on the Illumina Hi-seq 2500 following the manufacturer's protocols to obtain 50bp paired end reads.
GEO Accession
GSM3856445