GSM2831543: WT129_RNASeq; Mus musculus; RNA-Seq
RNA was extracted from 5 million cells with TRI Reagent (Sigma, 93289) according to manufacturer's instructions. Sample quality was assessed using a Bioanalyzer and quantified by Qubit. All samples were processed starting with 500 ng of total RNA according to the Illumina Stranded Total RNA protocol (15031048 Rev C, Sept 2012) except that 12 cycles of amplification was used to minimise amplification artefacts. Libraries were quantitated by Qubit and qPCR, and an equimolar pool were made based upon qPCR results. Following denaturation, 200 pM of the library pool was clustered in one lane of a HiSeq 3000 (Illumina Protocol 15006165 v02 Jan 2016) and 50 bp SR sequencing was carried out (Illumina Protocol 15066493 Rev A, February 2015).
GEO Accession
GSM2831543