Illumina Genome Analyzer IIx sequencing; Cavendish fruits 90 days after flowering (DAF), 4 days after a 24h acetylene treatment at 10,000 ppm
Banana fruits (cv. Cavendish, AAA triploid) were harvested at three developmental stages, namely 40, 60 and 90 DAF. After harvest, fruits were kept at 20C for 24 h and at least 10 fruits per development stage were subjected to treatment with 10,000 ppm of acetylene (an ethylene analogue) for 1 day, followed by 4 days storage in air. The control fruits were kept for 5 days in air at 20C.
cDNA libraries were constructed using the SMARTer PCR cDNA Synthesis Kit (Clontech, Saint-Quentin-en-Yvelines, France) according to the manufacturer's instructions, using 1 ug of DNase-treated total RNA. The cDNAs were sonicated to a 150- to 600-bp size range using the S2 Covaris instrument (Covaris, Inc., USA). Single end libraries were prepared using standard Illumina protocols (Illumina DNA sample kit). Each library was sequenced using the 76-base length read chemistry in a single flow cell on the Illumina GA IIx (Illumina, USA).