Structural and Biochemical Bases for Mannan Utilization by Caldanaerobius polysaccharolyticus- growth on mannose, rep 2
RNA isolated from two biological replicates were used for each growth condition. Bacterial ribosomal RNAs were removed from 10 µg of total RNA with the MicrobExpress kit (LifeTechnologies). The enriched mRNA fraction was converted to RNA-Seq libraries using the TruSeq Stranded RNA Sample Prep kit from Illumina. The barcoded libraries were pooled in equimolar concentration and the pool was analyzed by quantitative PCR and sequenced on one lane for 101 cycles on a HiSeq2000 using a TruSeq SBS sequencing kit version3. Fastq files were generated and demultiplexed with the bcl2fastq v1.8.4 Conversion Software (Illumina, Inc.)