GSM4182602: Apices, H3K4me3_WT_bio1; Arabidopsis thaliana; ChIP-Seq
Nuclei isolation and chromatin immunoprecipitation was performed according to Kaufmann et al 2010 Nature Protocols. After the preclearing step, the sample was split in three aliquots, the first aliquot was incubated with anti-H3K27me3 antibody (Active Motif, Cat. 39155, Lot. 25812014), the second aliquot was incubated with anti-H3K4me3 antibody (Millipore, Cat.17–614, Lot.1973237) and the third aliquot with anti-H3 antibody (ab1791, abcam) ChIP Libraries were prepared with the Ovation Ultralow V2 DNA-Seq library preparation kit from Tecan Genomics
GEO Accession
GSM4182602