GSM3015456: A2_RNA_Seq; Homo sapiens; RNA-Seq
Total RNA were immediately extracted using the RNeasy Universal Mini Kit (Qiagen) including DNase digestion. Resulting RNA quantity was assessed by Nanodrop 8000 (Thermo Scientific) and quality was assessed with the Agilent RNA 6000 Nano Kit on a 2100 Bioanalyzer (Agilent Technologies, Inc.) Libraries were prepared by the Van Andel Genomics Core from 500 ng of total RNA using the KAPA RNA HyperPrep Kit with RiboseErase (v1.16) (Kapa Biosystems, Wilmington, MA USA). RNA was sheared to 300-400 bp. Prior to PCR amplification, cDNA fragments were ligated to Bioo Scientific NEXTflex Adapters (Bioo Scientific, Austin, TX, USA). Quality and quantity of the finished libraries were assessed using a combination of Agilent DNA High Sensitivity chip (Agilent Technologies, Inc.), QuantiFluor® dsDNA System (Promega Corp., Madison, WI, USA), and Kapa Illumina Library Quantification qPCR assays (Kapa Biosystems).
GEO Accession
GSM3015456