GSM3543476: AGRP RiboTag Fasted ZT14 Collection 2; Mus musculus; RNA-Seq
Cell suspensions were dissociated from Vibratome slices of the ARH nucleus from NPY-GFP mice and FACS Sorted for GFP expression. RiboTag samples were immunoprecipitated using an established protocol (Sanz et al., 2009) and purified using Qiagen Rneasy Micro Kit. All samples were amplified using Takara Clontech SMART-seq v4 Ultra Low Input RNA Kit for sequencing. Amplified samples were constructed into sequenicng libraries using Illumina Nextera XT prep kit with manual normalization. Samples were quantified using BioAnalyzer and Qubit.
GEO Accession
GSM3543476