GSM2908871: 7-22 M6S T1; Mus musculus; OTHER
The genomic DNA was extracted using QIAGEN AllPrep DNA/RNA Kit (Cat. 80204). Integrity of genomic DNA was assessed by electrophoresis on 1% agarose gels, and concentration was determined by nanodrop. gDNA was sonicated into 500bp fragments and purified using Genomic DNA Clean & Concentrator Kit (ZYMO RESEARCH). The DNA library was prepared using Ovation Target Enrichment System (NuGEN) following the manufacturer's instructions. Target genes were selected from human HCC and mouse sequencing studies (Bard-Chapeau et al., 2014; Keng et al., 2009; Shen et al., 2016) and some well-known cancer related genes. The target probes were synthesized by NuGEN. More than 99.5% of the probes had >90% coverage. The sequencing was performed using a 150 bp single-end protocol on Illumina NextSeq 500 platform.
GEO Accession
GSM2908871