GSM1622604: mock-challenged plants; CK, T1; Boehmeria nivea; RNA-Seq
To prepare samples for Illumina sequencing, total RNA of each sample was extracted using an EASYspin plus Total RNA kit (Aidlab, Beijing, China) following the protocol. Quality and yield of RNA were determined by agarose gel electrophoresis and Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA), respectively. RNA of CK (equally mixed RNA of 4 time points) and CH (equally mixed RNA of 4 time points) samples were used to construct the cDNA library, respectively, with a fragment length range of 200 bp (±25 bp). Then, the paired-end sequencing was performed by using the Illumina sequencing platform (HiSeqTM 2000) according to the manufacturer’s instructions (Illumina, San Diego, CA).
GEO Accession
GSM1622604