GSM1519616: RNA-seq of the corpus callosum #5308; Homo sapiens; RNA-Seq
Tissue blocks were provided from NICHD brain bank. Total RNA was extracted from flash frozen tissue samples using Trizol reagent. Then the total RNA was treated with RNase-Free DNase (Qiagen) followed by purification with RNeasy MinElute Cleanup Kit (Qiagen) following the manufacturer’s instructions. 2 µg of total RNA each sample was subject to RNA-seq library preparation with ScriptSeq™ Complete Gold Kit from Epicentre (Cat. # SCL24EP, Madison, WI) following the manufacturer’s instructions. In brief, ribosomal RNA was depleted from total RNA using Ribo-Zero magnetic beads, then the ribosomal RNA depleted RNA was purified and fragmented. Random primer tailed with Illumina adaptor was used to perform reverse transcription to get cDNA library. Adaptor sequence was added to the other end of cDNA library with a Terminal-Tagging step. cDNA library was amplified with Illumina primers provided with this kit. The product was size selected (350~500 bp) from 2% agarose E-gels (Invitrogen) and sequenced in 1 lane per sample on Illumina’s HiSeq 2000 platform.
GEO Accession
GSM1519616