GSM2407774: 2489_4STARCH_Vogels2; Neurospora crassa; RNA-Seq
After 4 hrs in the new carbon source, the mycelia were harvested over a filter, flash frozen in liquid nitrogen and stored at −80°C. Total RNA was extracted using Phenol Chlorophorm, ethanol precipitated, and DNased and cleaned on a Qiagen column. Single end libraries were prepared for RNA sequencing (RNA-seq) using an Illumina kit (RS-100-0801) following standard protocols as described in Coradetti et al. (Coradetti et al, 2012). The cDNA libraries were sequenced on the Illumina HiSeq 2000 platform at the Vincent J. Coates Genomic Sequencing Laboratory, University of California, Berkeley.
GEO Accession
GSM2407774