GSM1621277: WNT6_Control_hg19; Homo sapiens; OTHER
4C-seq libraries were generated from microdissected tissues or cells as described previously (van de Werken et al., 2012). BglII or HindIII (6-bp cutters) were used as primary restriction enzymes. Csp6I or DpnII were used as secondary restriction enzymes. For each viewpoint, a total of 1.6 mg of each library was amplified in 8 or 16 PCR reactions, depending on PCR efficiencies. Samples were multiplexed and sequenced with Ilumina Hi-Seq technology. The following primers were used: WNT6_promoter_forward: CTACACGACGCTCTTCCGATCTGCAGCACTCACTCTAGCC, WNT6_promoter_reverse: CAGACGTGTGCTCTTCCGATCTGTTCGGAACCCAGACAGT, IHH_promoter_forward: CTACACGACGCTCTTCCGATCTAGAGTTGTTGAAAGGGTTAAAA, IHH_promoter_reverse: CAGACGTGTGCTCTTCCGATCTCTTGTCTGGTTGTGGCTTAC, PAX3_promoter_forward: CTACACGACGCTCTTCCGATCTGATGAATGTTCAAACCCAGAT, PAX3_promoter_reverse: CAGACGTGTGCTCTTCCGATCTGCGTGGAGTCAGAAGGAGT Samples were multiplexed and sequenced with Illumina Hi-Seq technology according to the manufacturer´s protocol.
GEO Accession
GSM1621277