Illumina MiSeq sequencing; qiita_ptid_1935:10585.SBS3PT
We evaluated multiple conditions including Igf1r inhibition in proximal and distal segments after intestinal resection to evaluate the epithelial effects of the absence of mechanoluminal stimulation.SBS was created in adult male zebrafish by performing proximal stoma to distal intestinal ligation. These were compared to sham zebrafish.
V4 Illumina MiSeq, 151f/806rbc
barcode
ACGCACATACAA
center_name
CHLA
linker
GT
mastermix_id
14509333
pcr_primers
FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
primer
GTGTGCCAGCMGCCGCGGTAA
primer_date
22114
primer_plate
5
processing_robot
JERE
run_date
5/6/13
sample_center
CHLA
sample_plate
CHLA_Plate1
sequencing_meth
Sequencing by synthesis
target_gene
16S rRNA
target_subfragment
V4
tm300_8_tool
111569B
tm50_8_tool
311426B
water_lot
RNBC2791
well_description
CHLA_Plate1_AX_E6
well_id
E6