GSM3611793: 2019-1; Mycobacterium tuberculosis; RNA-Seq
M. tuberculosis culture was harvested after centrifugation. Following washing with pre-chilled PBS, the pellet was lysed by TRIzol and total RNA was extracted by addition of chloroform and RNeasy mini kit. Illumina Ribo-Zero rRNA Removal Kit (Bacteria) was used for the removal of ribosomal RNA according to the manufacturer's protocol. The Illumina compatible RNAseq library was prepared using NEBNext ultra RNAseq library preparation kit. The cDNA libraries were purified using AmpureXP beads and quantified on an Agilent 2100 Bioanalyzer as well as a Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA). An equimolar amount of barcoded libraries were pooled and sequenced on Illumina NextSeq 500 (Illumina, San Diego, CA) with 1x75 configuration
GEO Accession
GSM3611793