GSM2409654: L 03/12 biological replica 3 (light); Acinetobacter baumannii; RNA-Seq
Total RNA was extracted as follows: Cells were pelleted and immediately mixed with 1 ml lysis buffer (0.1 M sodium acetate, 10 mM EDTA, 1% SDS) in a boiling-water bath. Cell lysates were extracted twice at 60 °C with one volume of phenol, which was adjusted to pH 4.0 with 50 mM sodium acetate, and then once with chloroform at room temperature. The RNA precipitated overnight at -20 °C with 2.5 volumes ethanol was collected by centrifugation, washed with 70% ethanol, and dissolved in DEPC-treated deionized water. Any possible residual gDNA was removed and the RNA was cleaned using Quick-RNA Miniprep according the manufacturer’s recommendation (Zymo Research). RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM2409654