GSM4561642: Javiana rep 1; Salmonella enterica subsp. enterica serovar Javiana; RNA-Seq
RNA protect was added to samples, followed by a 5 minute incubation at room temperature and cell collection. Collected cells were treated with lysozyme and proteinase K, followed by phase extraction with TRI Reagent Total RNA was DNA depleted using Turbo DNase, followed by rRNA depletion using Illumina RiboZero Bacterial Kit. 100 ng rRNA-depleted RNA was used as input for library prep with the NEBNext Ultra II Directional RNA Library Prep Kit.
GEO Accession
GSM4561642