GSM4065392: CS15_yolk_sac_10x; Homo sapiens; RNA-Seq
The human yolk sacs were carefully dissected under the microscope. After washing in PBS 3 times to remove any residual blood contamination, the yolk sacs were transferred to pre-warmed digestion medium containing 0.1 g/mL Collagenase I (Sigma, C2674, preheated to 37 °C) in RPMI 1640 medium. The samples were enzymatically digested at 37 °C in a humidified incubator for 20-30 minutes, with the samples being shaken periodically every five minutes until digested to single cell suspension. The cells were then filtered through a 70 mm cell strainer after neutralizing enzymes. The following antibodies were used for staining of the embryonic cells: CD235a (APC-Cy7, 349116 Biolegend) and 7-amino-actinomycin D (7-AAD) (PerCP-Cy5.5, 00699350 eBioscience).Cells were sorted with the Aria 2 Flow Cytometer (BD Bioscience). Data were analyzed with BD FACSDIVA V8.0.1. 10x Genomics Chromium Single Cell 3' Reagent Kit (v2 chemistry)
GEO Accession
GSM4065392