GSM1403174: HA Vasa DQAD IP - 12-mer replicate #1; Bombyx mori; ncRNA-Seq
Nucleic acids were isolated from protein complexes immunoprecipitated with the indicated antibodies and resolved by 15% or 20% urea-PAGE. Bands corresponding to piRNAs or the indicated size were excised from the gel and extracted with 400 µl of 0.3M NaCl solution at 25°C overnight. All deep sequencing libraries (except for JX52, JX59 and JX86) were prepared using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Cat No:E7300) according to manufacturer instructions. JX52, JX59 and JX86 were prepared by ligating homemade RNA adaptors, followed by gel-purification and RT-PCR. Libraries were sequenced on Illumina HiSeq platform (EMBL Heidelberg Gene core facility) or Illumina MiSeq platform (EMBL Heidelberg Gene core facility) .
GEO Accession
GSM1403174