GSM3856424: FSF_epi_1; Ambystoma mexicanum; RNA-Seq
Regenerating tissue was isolated from limbs at 5 dpa. Epidermis and stump tissues were separate dissociated, DAPI stained, and FACS sorted based on cell cycle content. RNA was extracted using the RecoverAll Total Nucleic Acid Isolation Kit for FFPE. More details can be found in Tsai et al. (2019). cDNA was synthesized with the NuGEN Ovation RNA-seq System V2, mechanically sheared to approximately 200 bp, and libraries were prepped using the Wafergen PrepX Complete ILMN DNA Library kit and the Apollo 324 NGS Library Prep System.
GEO Accession
GSM3856424