Illumina Genome Analyzer IIx sequencing; Acetyl-H4 chIP-seq of control, NaBu-treated, and random behavioral clusters of zebrafish 8 dpf larvae
Acetyl-H4 chIP-seq of control, NaBu-treated, and random behavioral clusters of zebrafish 8 dpf larvae
Zebrafish were isolated during pharyngula stage, and maintained until 8 dpf with daily changes of food and water 2 mM NaBu for 24 hours in specific samples ChIP protocol was performed as described in Roman et al. (Biorxiv 2017, doi: https://doi.org/10.1101/100230) using an anti-acetylH4 antibody from Millipore (#06-866). The chIP-seq libraries were built using the Truseq ChIP library preparation kit by Illumina (IP-202-1012) following manufacturer recommendations for Illumina GA II by the Genomics Unit of the Madrid Scientific Park.
Experimental Factor: immunoprecipitate
anti-acetylH4 (Millipore #06-866)