GSM2830939: gsrN-KO_rep1; Caulobacter vibrioides; RNA-Seq
Cultures were pelled and RNA was harvested using Trizol reagent. RNA was then immobilized on an RNeasy Mini Kit column (Qiagen 74106) and treated with TURBO™ Dnase (ThermoFisher AM2238) Ribosomal RNA was reduced using 4 ug of total RNA according to Gram negative Ribo-Zero rRNA Removal Kit (Illumina, CA). 100 ng of rRNA reduced RNA was processed  to strand specific RNA-Seq library construction, following Ovation Complete Prokaryotic RNA-Seq DR Multiplex System (Nugen, CA)
GEO Accession
GSM2830939