Illumina HiSeq 2000 sequencing; hMeDIP-seq of mouse intestinal stem and differentiated cells
hMeDIP-seq of mouse intestinal stem and differentiated cells
Lgr5-EGFP-IRES-CreERt2 mice used to isolate Lgr5+ intestinal stem cell (GFP+ cell sorted by FACS), Villus differentiated cells were collected by gentle scraping Using NEBNext® DNA Library Prep Master Mix Set for Illumina®
Experimental Factor: Lgr5 positive stem cell
cell type
Experimental Factor: input DNA
immunoprecipitate