ddRADseq of Phyllaplysia taylori: adult tail tissue
A modified protocol from Peterson et al. 2012 was followed, using the restriction enzymes SphI-HF and MluCl. Genomic DNA was isolated from frozen tail tissue using the E.Z.N.A. Mollusc DNA extraction kit from Omega Biotek. Samples were indexed and combined into twelve libraries, which were also labeled with two sets of indexes. These libraries were size-selected using a Pippin Prep to a 100bp section between 500bp and 600bp. Libraries were pooled and sequenced in two 100SR lanes on the Illumina HiSeq 4000.