UCE target enrichment of Colastomion sp.
Genomic DNA was extracted using a non-destructive technique  with the EZ-10 Spin Kit minipreps DNA Genomic Column Kit (BIOBasic, Toronto,  Ontario, Canada) following the manufacturers protocol. DNA fragmentation was  performed with the Qsonica Q800R sonicator (Qsonica 457 LLC, Newton, CT). The  DNA quantification was performed using a Qubit 2.0 fluorometer (Invitrogen,  Life Technologies, CA, USA). Genomic libraries were prepared following the  protocol described by Branstetter et al. (2017) using the Kapa Hyper Prep kit  (Kapa Biosystems Inc., Wilmington, MA), and the TruSeq-style dual-indexing  adapters (Glenn et al. 2016).