DNA-seq of Plinia trunciflora
Leaves were used to isolate chloroplasts by  the modified high salt method, followed by cpDNA extraction with the CTAB  method. DNA quality was evaluated by electrophoresis in a 1% agarose gel, and  DNA quantity was determined using a NanoDrop spectrophotometer (NanoDrop  Technologies, Wilmington, DE, USA). For each species, one genomic paired-end  library of 150 nt length was generated using an Illumina HiSeq2000 platform.