PacBio Sequel Genomic sequencing on Coho salmon
DNA was extracted from whole-body cross-sections, originally corrected by freezing immediately on Dry ice and placing in the -80C freezer for long term storage. DNA was extracted using standard Phenol and Chloroform method, with ethanol precipiatation using a shephard's crook to maximize fragment length. PacBio library prep followed standard sheared large-insert preparation guidelines, with both library prep and sequencing performed by the McGill University and Genome Quebec Innovation Centre in Montreal, Qc, Canada.