GSM3855053: MO DN rep2; Danio rerio; RNA-Seq
whole embryos were prepaired for FACsorting. A min. of 3000 cells were FACsorted directly into RLT buffer and RNA was isolated with the RNeasy Plus Micro Kit (QIAGEN, Cat No. 74034). High quality RNA (RIN > 8.0) was sent for RNAseq to the Wellcome Trust Centre for Human Genetics (WTCHG). 2.2 ng of total RNA was used for to generate SMARTer libraries for low-input RNA.
GEO Accession
GSM3855053