Illumina MiSeq sequencing; The genome-scale DNA-binding of FadR transcription factor in Sulfolobus acidocaldarius
The genome-scale DNA-binding of FadR transcription factor in Sulfolobus acidocaldarius
cells were crosslinked at OD600 0.2, fragmented by sonication. Then the cleared lysate were mixed with Saci_1107 specific antibody, pre-immune serum, respectively.  Immunoprecipitated DNA is extracted by IPure kit (Diagenode) according to the manufacturer's instructions Sequencing libraries were prepared using the ThruPLEX®-FD Prep Kit (Rubicon Genomics) under instructions from the manufacturer.
Experimental Factor: immunoprecipitate
mock