GSM3386292: Ad-GFP Iso3 rep3; Mus musculus; RNA-Seq
RNA sequencing was performed in primary neurons isolated from Tbx3loxP/loxP mice and treated with Ad-Cre or Ad-GFP viruses. Sequencing was performed in three independent neuronal isolations. One μg of total RNA per sample was used for library preparation Library construction was performed as described in the Low Throughput protocol of the TruSeq RNA Sample Prep Guide (Illumina) in an automated manner, using the Bravo Automated Liquid Handling Platform (Agilent). cDNA libraries were multiplexed and sequenced as 100 bp paired-end runs on an Illumina HiSeq2500 platform.
GEO Accession
GSM3386292