10X Chromium sequencing on Coho salmon: Library 1
A 10X chromium library was prepared from whole-body cross section from a single female Doubled-haploid individual from Dr. Robert Devlin at the Fisheries and Oceans Canada Pacific Science Enterprise Centre (West Vancouver, BC). DNA was extracted by liquid nitrogen grinding, phenol/chloroform/isoamyl alcohol (25:24:1, 3X), and finally purified by ethanol precipitation with Shephard's crook. DNA was sent to McGill University and Genome Quebec Innovation Centre for 10X library construction (multiplex SI-GA-D1) and sequencing on an Illumina HiSeq X instrument with PE 150bp reads.