DNA-Seq of Ustilaginoidea virens: rice grain false-smut disease causing fingi
The paired-end sequencing library was prepared using TruSeq Nano DNA Library Prep Kit(Illumina). Approximately 200 ng of DNA was fragmented by Covaris M220 to generate amean fragment distribution of 400 bp. The product was subjected to end repair followed byadopter ligation to the fragments.The size selected product was PCR amplified with index primers followingmanufacturers protocol.