GSM4066017: PRC1_GV_Ring1.Rnf2.DKO.Zp3_rep1; Mus musculus; RNA-Seq
For RNA isolation, 15 GV oocytes from one mouse were pooled and RNA was extracted using the RNAEasy Micro Kit (Qiagen,74004) according to the manufacturer's instructions. Total RNA was quantified and checked for quality on Agilent's BioAnalyzer using the RNA 6000 Pico Kit (Agilent, 5067-1513). RNA was amplified and converted to cDNA using the Ovation v2 Kit (NuGen, 7102). Sequencing libraries were prepared with the Truseq DNA LT kit (Illumina, FC-121-2001) and multiplexed, barcoded libraries were sequenced on an Illumina HiSeq instrument.
GEO Accession
GSM4066017