GSM1706799: H2217-CAT8.AFT2-GLU.2.XYL.5-4-3-03.20.14-1169_redo-5-59; Saccharomyces cerevisiae; RNA-Seq
Total mRNA was isolated using the yeast RiboPure kit (Life Technologies, Carlsbad CA). Libraries for RNA-Seq were prepared as in (Haynes, et al., 2011) except that the selection of poly(A) RNA from the total RNA isolated was accomplished using an mRNA Catcher Plus Kit (Life Technologies) and the resulting poly(A) RNA was subsequently sheared by incubating in TURBO DNA-free buffer at 75°C for 10 minutes and purified with a QIAquick PCR Purification Kit (Qiagen).
GEO Accession
GSM1706799