GSM3385670: HDM_allergy_Control_whole lung [MS0397]; Mus musculus; RNA-Seq
Tissues were collected in TRI-Reagent (Sigma-Aldrich) (for Toxoplasma gondii samples –tissues were collected and samples were stored in RNAlater (Ambion), and then transferred to TRI-Reagent). Total RNA was extracted using the RiboPure™ Kit (Ambion). Total RNA (200 ng) was used to prepare cDNA libraries using the TruSeq Stranded mRNA HT Library Preparation Kit (Illumina). Quality and integrity of the tagged libraries were initially assessed with the HT DNA HiSens Reagent kit (Perkin Elmer) using a LabChip GX bioanalyser (Caliper Life Sciences/Perkin Elmer). Tagged libraries were then sized and quantitated in duplicate (Agilent TapeStation system) using D1000 ScreenTape and reagents (Agilent). Libraries were normalised, pooled and then clustered using the HiSeq® 3000/4000 PE Cluster Kit (Illumina). The libraries were imaged and sequenced on an Illumina HiSeq 4000 sequencer using the HiSeq® 3000/4000 SBS kit (Illumina) at a minimum of 25 million paired-end reads (75 bp/100 bp) per sample.
GEO Accession
GSM3385670