Bicyclus anynana RNA-Seq
Standard lab stock females, reared at 19 or 27C (dry and wet season), were sampled 1 day after eclosion by snap-freezing in liquid nitrogen. RNA was isolated from abdomen or thorax tissue using TRIzol followed by Qiagen Rneasy (incl. DNase digestion). Library construction and sequencing was performed at BGI (People’s Republic of China).