GSM4066914: WT_16h_RNAseq; Mus musculus; RNA-Seq
Cellular RNA from ESCs and cells 16h and 32h after 2i withdrawal was purified using RNAeasy (Qiagen). For Zfp281 KO and corresponding WT cells, total RNA was subjected to ribosomal RNA depletion using Ribozero removal kit (Illumina) followed by library construction using ScriptSeq V2 library preparation kit (Illumina). For Ehmt1, Zic2, Ehmt1/Zic2 KO and corresponding WT cells RNA-seq libraries were prepared using TruSeq mRNA Library preparation kit (Illumina).
GEO Accession
GSM4066914