GSM3855867: Ross Rep 2; Gallus gallus; RNA-Seq
The extraction of total RNA from each muscle sample was performed using Trizol reagent (Invitrogen, USA), following the manufacturers protocol. The purity and concentration of RNA were detected by NanoDrop (Thermo Scientific™ NanoDrop 2000) and gel electrophoresis, respectively. The 28S/18S ratio for RNA samples ranged from 1.8 to 2. cDNA libraries were constructed using the TruSeq® RNA Sample Preparation Kit v2 (Illumina®, USA) and deep sequenced by the BGI Company (China, Shenzhen). Three RNA samples from the same breed were pooled (by mixing together equal quantities of RNA) to generate a total of 4 pooled RNA samples (two samples in each breed). An Illumina® HiSeq™ 2000 sequencer was used to sequence the libraries, according to the manufacturer's instructions, so as to generate paired-end reads of 150 base pair.
GEO Accession
GSM3855867