Ribo-seq of HEK293: 200 µM AS 2repl
Approximately 5 million HEK 293-TIA1 cells stressed with 200 µM AS for 30 min were used to isolate mRNA-bound ribosome complexes, followed by extraction of RNase I digestion-derived ribosome-protected fragments as described  {Ingolia, 2009} Cells were collected by flash-freezing without preincubation with antibiotics and the cDNA libraries from RPFs were prepared with direct ligation of the adapters and sequenced