RNA-Seq of Quercus ilex: combined stressed (salt + ozone) leaves replicate 4
Total RNA was isolated from leaves according to a CTAB method. RNAs were then purified by phenol/chloroform extraction (1:1 v/v) and precipitated following standard procedures. Then poly-A RNA was isolated from total RNA and was chemically fragmented. First- and second-strand cDNA syntheses were followed by end repair, and adenosines were added to the 3 ends. Adapters were ligated to the cDNA, and 200  25 bp fragments were gel purified and enriched by PCR. Then cDNA was sequenced by Illumina HiSeq 2000.