GSM1944222: Total RNA 0 hour; Mus musculus; RNA-Seq
RNA was extracted using TRIzol reagent, depleted of DNA using TURBO-DNase, and extracted with phenol-chloroform followed by precipitation with ethanol. For dsRIP samples, dsRNA was then immunoprecipitated with the J2 dsRNA antibody from English and Scientific Consulting. RNAs were fragmented to an average size of 150-200 nts prior to library preparation. cDNA libraries were generated using the Illumina TruSeq Stranded Total RNA Library Prep Kit according to manufacturer's instructions, except for the inclusion of additional DMSO for the reverse transcription reaction.
GEO Accession
GSM1944222