GSM2668654: Worms treated with cav-1 RNAi, rep 2; Caenorhabditis elegans; RNA-Seq
The worms were grown from hatching until day one of adulthood, collected with M9 buffer and washed in RNase-free water. The worm pellet was resuspended in 2 volumes of QIAzol (QIAgen, Hilden, Germany; #79306) and frozen overnight. Chloroform was used to separate RNA from protein and other materials. The RNeasy Lipid Tissue Mini Kit (QIAgen; #74804) was used according to the manufacturer instructions to extract RNA. Kapa stranded mRNA-Seq kit with mRNA capture Beads (kapabiosystems, KK8421, https://www.kapabiosystems.com/) was used. Briefly, 1ug was used for the library construction; library was eluted in 20ul of elution buffer. Libraries were adjusted to 10mM, then 10ul (50%) from each sample was collected and pooled in one tube.
GEO Accession
GSM2668654