GSM3990106: Small RNA sequencing in WT cells; Schizosaccharomyces pombe; ncRNA-Seq
Small RNAs between 15 and 30 nucleotides were extracted from 17% Urea-PAGE gels and eluted using Corning Costar Spin-X columns (Sigma Aldrich) followed by ethanol precipitation. NEBnext Small RNA Library for Illumina (NEB-E7330) was used according to the manufacturer's protocol Libraries were analyzed using an Agilent 4200 TapeStation system (Agilent) and sequenced on the Illumina MiSeq platform.
GEO Accession
GSM3990106