GSM3388052: m_H1; Sciaenops ocellatus; RNA-Seq
Pooled larvae were homogenized with a Kontes Pellet Pestle Cordless Motor (Sigma-Aldrich, St. Louis, Missouri) in RNAzol (Molecular Research Center, Cincinnati, Ohio).Total RNA was isolated and purified with RNeasy Mini Kit (Qiagen, Valencia, California) and quantified by NanoDrop ND-1000 Spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA). 200 ng of total RNA was used to prepare RNA-Seq libraries using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina following the protocol described by the manufacturer (Ipswich, MA).
GEO Accession
GSM3388052