Illumina HiSeq 2000 paired end sequencing; Illumina Sequencing of NBS-domains in 96 Potato Cultivars
For each cultivar, total genomic DNA was extracted, digested with 5 restrictases. Resistance gene fragments were isolated and amplified using 16 PCR primers targeting the nucleotide binding site (NBS) domain. After library preparation, indexed libraries were joined into four sequencing pools each consisting of 24 libraries, and run on the sequencer optimized for 100-bp paired-end sequencing. Each library has been uploaded as a bam file.
For library preparation, the PCR products were fragmented, ligated to library-specific indices including Illumina primers and adapters and then gel purified to obtain fragments in a range of 200 to 480 bp.