RNA-Seq. G. dorotocephala Postpharyngeal anterior Wound site / blastema. 12 hour post       injury. Rep #1.
"Libraries were prepared as previously described (Engreitz et al., 2014;         Schwartz et al., 2014). Briefly, RNA was extracted with TRIzol and following polyA selection         (Dynabeads) RNA was fragmented. Then, RNA oligo, corresponding to truncated Illumina primer,         was ligated to the 3' end of the fragmented RNA and reverse transcription was performed with         complementary sequence. Following RNA digestion, a second oligo was ligated to the cDNA.         Finally, libraries were enriched and barcoded by 9-12 cycles of PCR with complete Illumina         I7 and I5 oligos."