16S amplicon sequencing of an anaerobic biogas reactor digesting Scenedesmus spp. microalgae: thermophilic Continuous Stirred Tank Reactor (CSTR) day 126
DNA was extracted from 1 mL of sample using the MagMidi kit from LGC Genomics with bead beating in a MagNaLyser machine at 6500 rpm for 40 s. DNA was amplified with the primers Pro341F (5’-CCTACGGGNBGCASCAG-3’) and Pro805R (5’-GACTACNVGGGTATCTAATCC-3’) which amplify both bacteria and archaea. The Nextera XT DNA Library preparation kit was used to prepare the libraries which were sequencing on an Illumina MiSeq with readgent kit v3 (600-cycle) according to the manufacturer's protocol.