GSM3930435: GYY+LPS_3; Homo sapiens; RNA-Seq
Total RNA extraction was performed from 1.5-2х106 cells (THP1 line) with Extract RNA reagent (Evrogen, Russia). The concentration of isolated total RNA was measured with a Qubit Fluorometer (Invitrogen, USA). The quality of RNA was determined with an Agilent BioAnalyzer 2100 using an RNA 600 nano kit (Agilent, USA) Libraries for RNA-seq were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, USA) according to the manufacturer's guidelines. Seventy-five bp single-end sequencing was conducted on an Illumina NextSeq 500 platform