GSM3931503: Spl PB 1; Mus musculus; RNA-Seq
Single cell suspensions from spleen and bone marrow were depleted of erythrocytes using ACK lysis buffer and stained with a live/dead marker and the respective antibodies in PBS. Three replicates of each cell population (1,000 cells each) were sorted dire Un-stranded, non-rRNA, non-polyA+ selected libraries were prepared using the SMARTer Ultra Low Input RNA kit for Sequencing v3 (Clontech Laboratories). RNA sequencing: The libraries were sequenced on the Illumina HiSeq 2000 platform as 50 bp paired-end runs
GEO Accession
GSM3931503