GSM3854752: sRNA-Seq of stool sample from the colorectal adenoma patient A27; Homo sapiens; ncRNA-Seq
RNA was extracted using the Stool Total RNA Purification Kit (Norgen Biotek Corp). The RNA quality and quantity were verified according to the MIQE guidelines (http://miqe.gene-quantification.info/). The RNA concentration was quantified by Qubit with Qubit microRNA Assay Kit (Invitrogen). Library preparation was performed with the NEBNext Multiplex Small RNA Library Prep Set for Illumina (Protocol E7330, New England BioLabs Inc., USA). For each sample, 250 ng of RNA was used as starting material to prepare libraries. Multiplex adapter ligations, reverse transcription primer hybridization, reverse transcription reaction and the PCR amplification were processed with regard to the protocol for library preparation. After PCR pre-amplification, the cDNA constructs were purified with the QIAQuick PCR Purification Kit (Qiagen, Germany) following the modifications suggested by the NEBNext Multiplex Small RNA Library Prep Protocol. Further quality control check and size selection were performed following the NEBNext Multiplex Small RNA Library Prep Protocol (Protocol E7330, New England BioLabs Inc., USA). A concluding Bioanalyzer 2100 run with the High Sensitivity DNA Kit (Agilent Technologies, Germany) permitted to check DNA libraries final size, purity, and concentration.
GEO Accession
GSM3854752