GSM2409723: SATB1_input_rep2; Mus musculus; ChIP-Seq
Cells were subjected to crosslinking and nuclei were lysed. Nuclei sample was sonicated using a model XL2000 ultrasonic cell disruptor (MICROSON). Sonicated chromatin was incubated overnight at 4 °C with anti-SATB1 antibody (Abcam, ab70001) that was preconjugated with magnetic beads (Dynabeads). Immunoprecipitated DNA was then purified. Libraries were prepared according to NEB's instructions accompanying the NEBNext ChIP-seq Library prep Kit (E6240). The purified library was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols.
GEO Accession
GSM2409723