GSM2830933: Antler tip 1; Cervus nippon; miRNA-Seq
Total RNA was extracted using TRIzol (Invitrogen) according to the manufacturer's instructions. RNA integrity was assessed using the 2100 Bioanalyzer (Agilent Technologies) with RNA integrated number (RIN) values greater than or equal to 8 MiRNA-sequencing was performed by Nanjing Geneseeq Inc., Nanjing, China. Sequencing libraries were generated using NEBNext Multiplex Small RNA Library Prep Set for Illumina(NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample
GEO Accession
GSM2830933