16s pro seq plant and soil
From the extracted DNA, primers 519F '5- CAGCMGCCGCGGTAA-3' and 786R 5'-TACNVGGGTATCTAATCC-3' were used to generate prokaryotic 16S amplicons through PCR. Paired-end sequence data were generated on an Illumina MiSeq instrument using v3 600 cycle kits, with dual 6 bp dual index sequences attached to each amplicon during indexing PCR as described in the Illumina 16S Metagenomic Sequencing Library Preparation protocol.