Targeted-Capture  of Rubus subgenus Rubus:High brix  :Oregon:Leaf
Genes  associated with fruit sugar production in blackberry were identified using a  family-level candidate gene approach. Rubus transcripts were identified from  the Rubus reference transcriptome (https://www.rosaceae.org/analysis/227)  using sugar candidate genes from apple (Malus domestica), peach (Prunus  persica), and alpine strawberry (Fragaria vesca). These genes were used to  design Hyb-Seq baits at a 2X tiling density. The baits were used in  conjunction with PacBio sequencing to genotype 40 cultivars with high and low  soluble solids content (SSC) from the University of Arkansas and USDA  blackberry breeding programs. Sequencing and filtering of the captured  genomic reads for the 40 samples produced circular consensus (CCS) reads.