GSM2830912: PBMC 1 day after treatment, 2nd series; Homo sapiens; FAIRE-seq
PBMCs were isolated within one hour after blood draw from 8 ml of peripheral blood in a Vacutainer CPT Cell Preparation Tube with sodium citrate (Becton Dickinson, Franklin Lakes, NJ, USA) according to the manufacturer's instructions. FAIRE analysis was performed according to the protocol published by Giresi et al with some modifications (crosslinking with formaldehyde, cell lysis, phenol-chloroform extraction, reverse-crosslinking, purification). FAIRE-seq library was prepared from 10 ng ChIP DNA with the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) per manufacturer's protocol
GEO Accession
GSM2830912