GSM2667988: WT-mock; Human immunodeficiency virus 1; RIP-Seq
Virions collected from cells were pelleted on sucrose cushions, lysed in detergent containing buffers and IN-RNA complexes were immunoprecipitated from these lysates. Libraries were constructed based on previously published methodologies as explained before (Kutluay et al,m 2014, Cell). The 3' adapter is common to all samples and has the sequence: TCG TAT GCC GTC TTC TGC TTG. The 5' adapters are:Sample 1 (rGUU CAG AGU UCU ACA GUC CGA CGA UC GAU NNN UC); Sample 2 ( rGUU CAG AGU UCU ACA GUC CGA CGA UC GUG NNN UC); Sample 3 ( rGUU CAG AGU UCU ACA GUC CGA CGA UC UAG NNN UC); Sample 4 (rGUU CAG AGU UCU ACA GUC CGA CGA UC AUC NNN UC).
GEO Accession
GSM2667988