GSM4498089: MCF-10A Onco M + HGF + IFN-Y (RNA seq); Homo sapiens; RNA-Seq
Total RNA was extracted with Trizol (Invitrogen) according to the manufacturer's instructions. RNA obtained was further purified using QIAGEN RNeasy columns with DNase I treatment according to the manufacturer's instructions. Libraries construction and amplified libraries were purified with SizeSelector-I beads (Aline Biosciences) as were prepared as described (Jin et al., 2015). Purified barcoded libraries were then examined and quantified via Bioanalyzer (Agilent). RNA-seq libraries were sequenced by Harvard Bauer Core Facility using Hiseq 2000. Sequence analysis was performed as described (Yi J, et al. 2015). RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM4498089