GSM4561670: AAT_exposed_epiCS; Homo sapiens; RNA-Seq
The total RNA from epiCS was isolated, and quality was assessed using 1% agarose gels as well as by Agilent 2100 Bioanalyzer using Agilent RNA 6000 Nano Kit Libraries were prepared in duplicates by using 200 ng RNA of each insert. TruSeq Stranded mRNA Kit (Illumina Inc., USA) was used for library preparation according to the manufacturer protocol as described in our previous work.  The products were purified with Ampure XP Beads (Beckman Coulter, CA, USA) and enriched with 15 cycles of PCR to create the cDNA library. Pair-ended RNA-Seq
GEO Accession
GSM4561670