GSM2224443: CBFB-MYH11_-Dox_Runx1-rep2; Mus musculus; ChIP-Seq
Lysates were clarified from sonicated nuclei following crosslinking with 1% formaldehyde, and histone-DNA complexes were isolated with antibody. Sequencing libraries were prepared using the TruSeq Kit (Illumina) for high throughput sequencing on an Illumina HiSeq 2000, according to manufacturer’s instructions, with size selection for fragments of 150-400 bp.