GSM1706504: Tip60_ChIPseq; Mus musculus; ChIP-Seq
mESCs were cross-linked with 1% formaldehyde for 10 minutes at room temperature, lysded and shared mechanically using the Covaris E210 to obtain a chromatin fragment size of 200-500bp. For the IP histone-DNA complexes 6 ug of the anti-Tip60 antibody mixture (RLPV, CLGT and CLHF polyclonal sera) was used, which was a gift from B. Amati. DNA was purified with Qiaquick (Qiagen) columns. Ten ng of precipitated DNA was used for Solexa sequencing. To create a genomic library we followed the instructions of the NEBNext protocol (Biolabs, E6240). Librarys were validated with the Agilent Bioanalyzer.
GEO Accession
GSM1706504