GSM1402770: LWT1; Mus musculus; RNA-Seq
Twelve 10-week-old males per genotype were fed with MCD diet for 3 weeks.  Liver tissue was dissected and immediately frozen in liquid nitrogen. Total RNA was extracted with QIAzol Lysis Reagent and then purified on RNeasy spin columns (Qiagen). RNA integrity (RNA Integrity Score ≥ 7) and quantity were determined on the Agilent 2100 Bioanalyzer. RNA samples from different animals were mixed in equal proportion at two samples per pool, and four pools per genotype were analyzed in the Illumina Genome Analyzer IIx. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM1402770