coliBOQ1_Medusa B.fasta genome
The reads quality was analyzed with FastQC (3) and Quality-based trimming was performed with a DynamicTrim (SolexaQA++) (4) perl script with a minimum Phred score of 20. The Illumina reads were assembled de novo using the SPAdes program (version 3.9.0) with k-mer odd values from 21 to 71 that generated 74 contigs. Contigs with length less than 500 bp were discarded and the remaining ones used for a multi-draft-based analysis using three Escherichia coli genomes (strains ATCC8739, BL21 DE3 and MG1655) through MeDuSa scaffolder (cita). The final version of the genome embeds 12 scaffolds, containing 4,601,531 bp with a general GC content of 50.89 %, where two of them are the plasmids pBOQ-IncQ (Scaffold_10_pBOQ-IncQ; 4,640 pb and 59% of GC content) and pBOQ-95LK (Scaffold_12_pBOQ-95LK; 95,980 pb and 47.8% of GC content).