GSM2062275: input DNA; Mus musculus; ChIP-Seq
Two biological replicates of pooled E18.5 lungs (n=3) were crosslinked with 37% formaldehyde to a final concentration of 1%. ChIP was performed using the SimpleChIP Enzymatic Chromatin IP Kit (Cell Signaling, 9005S). Micrococcal nuclease-digested chromatin was further sonicated using Diagenode Bioruptor with 20 pulses of 15s on and 15s off at high power to yield sheared chromatin. Ten micrograms of chromatin was used per immunoprecipitation with 7 µg of FOXF1 antibody. The Rubicon ThruPlex DNA-Seq library preparation system was used to prepare ChIP-Seq libraries for sequencing on the Illumina HiSeq sequencing system.
GEO Accession
GSM2062275