V6 and V1V2 16S rDNA PCR separatly run on template from the 2                     different negative DNA extraction controls, the 2 amplicons were pooled together                     and purified, measuring amount of amplicon DNA prior to                     sequencing.
V1V2 primers
A+V1_For:GCCTCCCTCGCGCCATCAGAGAGTTTGATCMTGGCTCAG ;                          B+V2_Rev:GCCTTGCCAGCCCGCTCAGCYNACTGCTGCCTCCCGTAG
V6 primers
A+1061_Rev:GCCTCCCTCGCGCCATCAGCRRCACGAGCTGACGAC ;                          B+784_For:GCCTTGCCAGCCCGCTCAGAGGATTAGATACCCTGGTA