GSM1704058: GEK12; Glycine max; ncRNA-Seq
Collected samples were immediately powdered in Trizol (Invitrogen, CA, USA) and stored until the RNA extraction. Total RNA was isolated using Trizol (Invitrogen, CA, USA), and following the manufacturer’s instructions. The RNA quality was evaluated by electrophoresis in 1.0% agarose gel and the amount of the RNA was checked using a Quibit fluorometer and Quant-iT RNA assay kit according to the manufacturer’s instructions (Invitrogen, CA, USA). Approximately 10 µg of total RNA was sent to Fasteris Life Sciences SA (Plan-les-Ouates, Switzerland) for processing and sequencing using Solexa technology on the Illumina Genome Analyzer GAII. Briefly, the processing by Illumina consisted of the following successive steps: acrylamide gel purification of RNA bands corresponding to the size range 20–30 nucleotides (nt), ligation of 3′ and 5′ adapters to the RNA in two separate subsequent steps each followed by acrylamide gel purification, cDNA synthesis and a final step of PCR amplification to generate DNA colonies template library for Illumina sequencing.
GEO Accession
GSM1704058