GSM4182496: H6-1mRNA; Homo sapiens; RNA-Seq
mRNA seq: Cytoplasmic fractions were collected using the nuclei isolation protocol.  The extracts were subjected to oligodT selection of polyadenylated mRNA using poly-A coated magnetic beads. PRO-seq: Nuclei isolation followed by Nuclear Run-on in the presence of biotinylated nucleotides (A, U, G, C).  Biotinylated transcripts were isolated using streptavadin coated magnetic beads mRNA seq: Libraries were constructed by Camille Abshire at Louisiana State University Health Science Center in Shreveport in the Genomics Core using the TruSeq Stranded mRNA LS kit. PRO-seq: Libraries were constructed following the protocol outlined by Mahat et al. 2016. "Base-pair-resolution genome-wide mapping of active RNA Polymerases using precision nuclear run-on (PRO-seq).  Nature Protocols. Aug 11(8):1455-76 doi:10.1038/nprot.2016.086
GEO Accession
GSM4182496