TnSeq 10 of haploid C. albicans
1g of genomic DNA from each insertion library was randomly sheared using a Covaris S2 device (Covaris Inc., Woburn, MA, USA). Single-end Illumina libraries were constructed by ligation of Illumina adapters to sheared DNA. Enrichment of transposon/chromosomal junction regions was performed by PCR amplification with a 5 biotinylated transposon enrichment primer, BP664, and adapter-specific PCR enrichment primer R_Tnseq_index. All the following transposon sequencing steps were performed as described [1, 2]. The resulting transposon libraries were quantified on an Agilent 2200 TapeStation system using HS D1000 tape and sequenced using Illumina NextSeq500 (Illumina, US) high output v2 kit.1. van Opijnen, T., K.L. Bodi, and A. Camilli, Tn-seq: high-throughput parallel sequencing for fitness and genetic interaction studies in microorganisms. Nat Methods, 2009. 6(10): p. 767-72.2. van Opijnen, T. and A. Camilli, Transposon insertion sequencing: a new tool for systems-level analysis of microorganisms. Nat Rev Microbiol, 2013. 11(7): p. 435-42.