Replacement of endogenous ethanol metabolism in Saccharomyces cerevisiae by an ATP-independent acetylating acetaldehyde dehydrogenase pathway
Illumina genome sequencing Genomic DNA libraries for the Illumina platform were generated and sequenced at BaseClear BV (Leiden, The Netherlands). High-molecular weight genomic DNA was used as input for library preparation using the Illumina TruSeq DNA library preparation kit (Illumina). Briefly, the gDNA was fragmented by nebulization and subjected to end-repair, A-tailing, ligation of adaptors including sample-specific barcodes and size-selection to obtain a library with median insert-size around 250-300 bp. After PCR enrichment, the resultant library was checked on a Bioanalyzer (Agilent) and quantified. The libraries were multiplexed, clustered, and sequenced on an Illumina HiSeq 2000 with paired-end protocol. The sequence fastq file contained interleafed paired end data. Submitted by: Delft University of Biotechnology