Whole genome sequencing of Saccharomyces cerevisiae SY43-6
Yeast strain SY43 expressing APOBEC3B were incubated on low-gal (0.005% galactose) plates (containing hygromycin) to form single colonies. One colony was purified on a high-gal (0.05% galactose) plate. The  purified colonies were grown on high-gal plates. The yeast cells were collected for genomic DNA extraction and whole genome sequencing by Illumina technology.