GSM4498279: Mut rep1; Mus musculus; RNA-Seq
Embryos used for Drop-seq profiling were harvested at E8.25 in 1x ice cold PBS, pooled with their littermates, dissociated with TrypLE (Fisher) reagent for 10 minutes at 37C while shaking at 1,400 rpm in a Fisher Thermomixer, followed by inactivation by addition of 10% FBS in 1x PBS. Cells were spun down at 800 x g for 5 minutes, re-suspended in 10% FBS with a Near-IR dead dye (Life Technologies) and strained through a 40µm mesh prior to FACS. Mesp1Cre-marked cells were isolated by FACS and collected in cold 1x PBS; 0.01%BSA solution. Drop-seq protocol was performed according to Macosko et al. with the modification that a custom-built 90 μm Drop-seq microfluidic device was used for droplet generation, creating droplets smaller than the standard Drop-seq protocol
GEO Accession
GSM4498279