Clostridium thermocellum LQRI genome sequencing
genomic DNA underwent Pacific Biosciences standard template preparation and sequencing protocols. Starting material was 5µg of gDNA in conjunction with the low input library preparation protocol. Library preparation selected > 10 kb fragments were selected for LQRI using Covaris g-Tubes and AMPure PB bead-based size selection. Version P6 sequencing polymerase was used. SMRTbell template libraries were sequenced on a Pacific Biosciences RSII sequencer with sequencing version C4 chemistry.