GSM2062997: B. pseudomallei K96243 bprS mutant replicate 1; Burkholderia pseudomallei K96243; RNA-Seq
Strains were subcultured 1/50 into fresh antibiotic-free medium and grown at 37 °C with shaking (200 rpm) to an OD600 of 0.5. The cells were harvested by centrifugation, and RNA purified using Trizol reagent (Gibco/BRL) as specified by the manufacturer. RNA was DNase treated using the TURBO DNA-free kit (Ambion), followed by processing with a QIAGEN RNeasy kit clean-up with on-column DNase digestion as per the manufacturer’s instructions. RNA samples were quantified and analysed for DNA contamination using the Qubit kit (Invitrogen) and visualised by gel electrophoresis. The construction of the cDNA library from purified RNA samples was performed using the Truseq RNA Preparation Kit (Illumina). Ribosomal RNA (rRNA) was removed from the samples using the Ribozero kit (Epicentre).
GEO Accession
GSM2062997