Transcriptome RNA-seq of non-cultured samples mimics mixing H1N1 RNA and human A549 cell line RNA by next-generation sequencing to evaluate the effects of pre-treatments on influenza genome recovery
Extraction of all RNA in the mixture sample. RT-PCR was performed and double-strand cDNA was prepared multiplex NGS libraries by using Nextera XT DNA Sample Preparation Kit (Illumina),and NGS was performed with an Illumina MiSeq platform.