WGS of Pseudomonas savastanoi pv. retacarpa CECT4861
Genomic DNA from Pseudomonas savastanoi pv. retacarpa CECT4861 was extracted from bacterial cells grown overnight in LB medium at 28ĄC. DNA was extracted using Genomic DNA Purification JETFLEX kit (Genomed GmbH, Lhne, Germany) following the manufacturerŐs instructions. The DNA sample was further purified by a first extraction with phenol:chloroform:isoamyl alcohol (25:24:1), followed by a second extraction with chloroform:isoamyl alcohol (24:1). DNA was precipitated with 1/10 volume of 3M sodium acetate and two volumes of 100% ethanol and resuspended in MilliQ water. NanoDrop measurements gave a concentration of 473,0 ng/ľL (in total 9,5 ľg of DNA) with A260/A280 of 2.00 and A260/230 of 2,43. The draft genome of this strain was sequenced using Illumina Genome Analyzer IIx at "Centro de Investigacin Biomdica de La RiojaÓ (CIBIR), Logroo, Spain.