GSM1520434: wildtype H37Rv experiment 1 replicate 1 5' end mapping library with triphosphate conversion; Mycobacterium tuberculosis; RNA-Seq
Cultures were inactivated with RNAlater, disrupted with bead-beating in the presence of Trizol, and total RNA was extracted according to the manufacturer's instructions. RNA was then treated with Dnase and re-purified with RNeasy. Expression libraries were constructed using either the dUTP method or a ligation-based method to maintain strand specificity. 5' end mapping libraries were constructed by direct ligation of adapters to RNA 5' ends after differential treatment with or without 5' polyphosphatase.
GEO Accession
GSM1520434