Ctrl-siRNA PolyA - Bisulfite Treated
Tryzol extraction of total RNA. Isolation of mRNAs using polyA Spinª mRNA Isolation Kit followed by additional RNA fragmentation. Samples are treated with sodium bisulfite mix for 16 hours at 50C. Directional (strand-specific) sequencing-library preparation protocol (Illumina) for RNA samples for RNASeq.