metagenome+stool: fed with human milk
The V3 and V4 regions of the 16S rRNA gene were amplified by PCR. The first PCR amplified the V3-to-V4 region of the 16S rRNA gene using the 341F and 806R primers: 5-CCTACGGGNGGCWGCAG-3 (forward primer) and 5- GGACTACHVGGGTATCTAAT -8 (reverse primer), where the barcode is an eight-base sequence unique to each sample.