PacBioRSII Raw-Reads of Galdieria sulphuraria Azora
To obtain axenic and monoclonal genetic material for sequencing, single colonies of each strain were grown at a temperature of 37C in the dark on plates containing glucose as the sole carbon source (1% Gelrite mixed 1:1 with 2x Allen medium, 50 M Glucose). The purity of single colonies was assessed using microscopy (Zeiss Axio Imager 2, 1000x) and molecular markers (18S, rbcL). Long read DNA was extracted using a genomic-tip 20/G column following the steps of the "YEAST". DNA extraction protocol (QIAGEN N.V., Hilden, Germany). The size and quality of DNA were assessed via gel electrophoresis and the Nanodrop instrument (Thermo Fisher Scientific Inc, Waltham, MA). DNA was sequenced in 2016/2017 using the PacBio RS2 (Pacific Biosciences Inc., Menlo Park, CA) technology.