GSM3387475: CFPAC1.FOXA2-KD.Rep2; Homo sapiens; RNA-Seq
10^6 cells are lysed and processed according to SMART-Seq2 protocol. Briefly, 10 ng of poly-A containing mRNA molecules are copied into first strand cDNA by reverse transcription and template-switching using oligo(dt) primers and LNA-containing template-switching oligo (TSO). cDNA is pre-amplified using limited number of cycles, purified and tagmented with Tn5 transposase. cDNA fragments generated after tagmentation are gap-repaired, enriched by PCR and purified to create the final cDNA library for HiSeq 2000 Illumina sequencing.
GEO Accession
GSM3387475