Illumina MiSeq sequencing; qiita_ptid_5458:12033.LAC39.w2.C15
Infant monkeys (from birth to age of 16 weeks) consumed either exclusive breastmilk or infant formula (standard or reduce protein, with or without additional of free amino acids)
Sequences were amplified using F515/R806 primer pair to target V4 region of 16S rRNA gene Both forward and reverse primers were modified to contain an illumina adapter region for sequencing on the illumina GaIIx platform and on the forward primer site, an 8 bp Hamming error-correcting barcode was used to enable sample multiplexing.
barcode
ACAGCTCA
center_name
UC Davis Slupsky Lab
center_project_name
mead_johnson_Infant_monkey_project
linker
GT
linkerprimersequence
GTGTGCCAGCMGCCGCGGTAA
pcr_cond
94degC_3min; denaturation:94degC_45sec; annealing:50degC_1min; elongation: 72degC_90sec; final elongation:72degC_10min; 40total cycles
pcr_primers
FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
primer
GTGCCAGCMGCCGCGGTAA
reverseprimer
GGACTACHVGGTWTCTAAT
run_center
UC Davis Genome Center
run_date
9/1/13
sequencing_meth
Sequencing by synthesis
target_gene
16S rRNA
target_subfragment
V4