GSM3930364: rpfs (GC7-treated); Mus musculus; OTHER
Harvested cells were lysed and spiked with S. cerevisiae lysate at the ratio of 13 million B cells: 270 million yeast cells (which equals to ~ 20:4 µg miRNA). Lysate was digested with RnaseI and monosomes were extracted using SEC. RNA was extracted rRNA was depleted using RiboZero and small RNAs were isolated using TBU-PAGE gel extraction. Libraries were constructed according to Ingolia et al., 2012.