16S DNA-Seq of Amblyomma americanum: adult female bacterial community-GPZ3F3401_CCGGCCA_TRM1007
Barcoded eubacterial 16S primers 357F and 926R were used to amplify variable regions 5-3 from whole tick DNA extract. Invitrogen AccuPrime Taq DNA Polymerase High Fidelity was used for primary PCR. Amplicons were sequenced on the Roche 454 FLX Titanium system. Library ID is formatted as follows: PlateID_SampleMID_SampleName.