GSM3611185: Cortical thymic epitethial cell, VEGF-Trap #3; Mus musculus; RNA-Seq
Cells were lyzed in QIAzol lysis agent (Qiagen cat# 79306) and RNA was extracted using the miRNeasy microkit (Qiagen cat# 217084 Hilgen, Germany) as per the manufacturer's instructions. Total RNA was quantified using the Agilent 6000 pico kit (cat#5067-1513) in the Agilent 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA). RNA sequencing and library preparation was performed by the UCLA Technology Center for Genomics and Bioinformatics. cDNA generation and amplification was made using the Ovation RNA-Seq System V2 (cat# 7102-32, NuGEN Technologies, San Carlos, CA). Library preparation was done using the KAPA LTP kit (# KK8232, Kapa Biosystems/Roche, Wilmington, MA), according to the manufacturer's instructions. The work flow consisted of mRNA enrichment, cDNA generation, end repair to generate blunt ends, A-tailing, adaptor ligation and PCR amplification. Different adaptors were used for multiplexing samples in one lane. Sequencing was performed on the HiSeq3000 System for a paired-ended 150bp run (Illumina).
GEO Accession
GSM3611185