Whole genome sequencing of Pongamia pinnata
Genomic DNA was fragmented using ultrasonicator (Covaris M220), ends were repaired using SureSelect XT2 Library Prep Kit and cleaned up using Agencount Ampure XP SPRI beads. DNA fragments was adenylated at 3' end. The indexed library was amplified, cleaned up and quantification was performed by Qubit fluorometer using DNA-HS kit. The libraries were loaded on different sequencing platforms including HiSeq, MiSeq and NextSeq.