rRNA depleted metatranscriptomic sequencing from 498 wild-caught adult Drosophila immigrans
Our objective was to identify virus-like sequences associated with Drosophila immigrans in the wild.  498 Adult flies were collected (63 flies, July 2011 Edinburgh 55.928N, 3.170W; 285 flies July 2011 Edinburgh N55.921, W3.193; 150 flies July 2011 Sussex 51.100N, 0.164E). RNA was extracted using Trizol, and submitted (Dry, in RNAstable) to BGI for rRNA depletion by Ribo-Zero and paired-end RNA sequencing.