Low-risk gastro-intestinal stromal tumor whole-genome sequencing
Tissues were homogenized and subsequently mixed with lysis buffer  (700 microlitre/gram tissue)  containing 2.1% (v/v) Proteinase K  (10mg/ml) and 0.7% SDS in TE (pH 8.0).  Samples were incubated o/n at 37°C. Phenol extraction followed by butanol treatment and ethanol precipitation was performed  as described by Sambrook and Russell.  A DNA library generated from 3 microgram of DNA  was sequenced using 7 lanes on an Illumina HiSeq 2000 machine.