GSM3611762: NW3; Mus musculus; MeDIP-Seq
DNA samples were fragmented to a size range of ∼200-800bp with a Diagenode Bioruptor. About 1 μg of fragmented DNA was prepared for Illumina HiSeq 4000 sequencing as the following steps: 1) End repair of DNA samples; 2) A single 'A' base was added to the 3' ends; 3) Ligation of Illumina's genomic adapters to DNA fragments; 4) hMeDIP to enrich Hydroxymethylated DNA by anti-5-Hydroxymethylcytosine antibody; 5) PCR amplification to enrich precipitated fragments; 6) Size selection of ∼300-900bp DNA fragments using AMPure XP beads. The completed libraries were quantified by Agilent 2100 Bioanalyzer.
GEO Accession
GSM3611762