HiSeq X Ten sequencing; Amplicon sequencing of genomically-encoded guide RNA sequences for the Brunello CRISPR library in eHap cells
Amplicon sequencing of genomically-encoded guide RNA sequences for the Brunello CRISPR library in eHap cells
eHap cells collected and either genomic DNA is immediately extracted or the cells are fixed, permeabilized, and then subjected to immunostaining flow cytometry for sorting. QIAmp DNA mini kit. PCR to attached adapters and illumina barcodes.
Experimental Factor: phenotype
RPS6p stained