GSM3541167: 11.6; Homo sapiens; RNA-Seq
RNA was extracted using miRNeasy mini kit (Qiagen) from each core. according the manufacturer's protocol. RNA concentration and integrity were measured by using NanoDrop ND-2000 and 2200 Tape Station 20 ng RNA input was used for library preparation by AmpliSeq™ Transcriptome Human Gene Expression kit according the manufacturer's protocol . The cDNA was then amplified and barcoded using the Ion Xpress™ RNA-Seq Barcode 1-16 Kit (Life Technologies 4475485).  The Ion PI™ Chip Kit v2 BC (Life Technologies) 4484270) was then loaded using the Ion Chef™ System (Life Technologies 4484177) with the Ion PI™ IC 200 Kit (Life Technologies 4488377). Afterwards, the chips were run on the Ion Proton™ System for Next-Generation Sequencing (Life Technologies 4476610) using the Ion PI™ IC 200 Kit.
GEO Accession
GSM3541167