Unstimulated thymus & bone marrow, & mitogen-stimulated spleen, whole blood and lymph node transcriptome
RNA extraction was carried out as previously described using the RNeasy mini kit (Qiagen) with removal of genomic DNA with DNaseI digestion. Total RNA from the thymus of a juvenile male bat was used for Illumina sequencing separately from all other samples. Total RNA obtained from the stimulated and unstimulated cells from the two adult bats was pooled as follows: 22% thymus total RNA (11% from each bat) and 78% pooled total RNA from the rest of the mitogen stimulated & unstimulated cells/tissues (~3.45% for each sample; total of 22 samples).