GSM4338200: Nil-95_Control_Panicle-6; Oryza sativa; RNA-Seq
Panicle tissue under control and drought-stress conditions were removed, flash frozen on dry liquid nitrogen, and total RNA was extracted using TRIzol reagent and isolated using the Qiagen RNeasy Plant Mini Kit (Qiagen, Limburg, Netherlands). The total RNA concentration was quantified using Nanodrop spectrophotometer (ND-1000; Nanodrop Technologies, Wilmington, DE), with absorbance at 260 nm. RNA purity and integrity were examined using an Agilent 2100 BioAnalyzer RNA 6000 Kit (Agilent Technologies). 2 ug of total RNA was submitted and Illumina library preparation and sequencing were completed following the standard protocols of Macrogen INC (Seoul, Korea). RNA libraries were prepared for sequencing using standard Illumina protocols as per Macrogen Korea specifications.
GEO Accession
GSM4338200