GSM3854155: ADAR1 E861A ADAR2 KO Adult Brain  - Whole. Replicate 3 (546) DKOr3; Mus musculus; RNA-Seq
[75bp] Post ribosome depleted RNA was purified and subjected to indexing and library preparation using the Kapa Stranded RNA-seq Library Preparation Kit for Illumina Platforms (Kapa Biosystems). Library amplification was performed for 9 cycles of PCR. The resultant libraries were pooled and sequenced on a single run using the Illumina NextSeq500 with 75bp paired end reads at the Ramaciotti Centre for Genomics (UNSW, Australia). [150bp] Total RNA was isolated from the whole brain from 12-week-old males Adar1E861A/+Ifih1-/-Adar2+/-Gria2R/R (dHet), Adar1+/+Ifih1-/-Adar2-/-Gria2R/R (Adar2-/-) and Adar1E861A/E861AIfih1-/- Adar2-/-Gria2R/R (Quad) mice (n= 3/genotype). Whole brain was lysed in Trisure and purified using DirectZol columns. Post ribosome-depleted RNA was purified and subjected to indexing and library preparation using the Kapa Stranded RNA-seq Library Preparation Kit (Kapa Biosystems) and sequenced at PE150bp on the Illumina platform by the Novogene (Hong Kong).
GEO Accession
GSM3854155