GSM2483424: MCF7_H3K4me3_Rep1_TCLSeq; Homo sapiens; ChIP-Seq
For ChIP samples,unfixed cells were clarified after enzymmatically digesting and lysing cells, then histone-DNA complexes were isolated with antibody. For TCL samples, cells were permeablized and treated with restriction enzymes to fragment chromatin, then diluted with lysis buffer.  Antibody-adapter complexes were added to chromatin fragment solution, then a ligation mix was added to facilitate proximity ligation of the antibody bound adapter.  DNA was puried and ligated DNA was amplified prior to library construction. Libraries were prepared with Illumina's NEXTERA DNA prep kit (FC-121-1031)
GEO Accession
GSM2483424