GSM3388391: Chipseq_Input_P14_Cl13_D7_TerminallyExhaused_1; Mus musculus; ChIP-Seq
cell isolation protocol: Cells from spleen were obtained by mechanical disruption. Isolated cells were further sorted based on their surface markers. Lymphocytic choriomeningitis virus infection protocol: For acute viral infections, mice were intravenously (i.v.) injected with 2×10^5 plaque-forming unit (PFU) LCMV Armstrong. For chronic viral infections, mice were intravenously (i.v.) injected with 2×10^6 PFU LCMV clone 13. One million FACS sorted cells were used for ChIP. DNA-protein crosslinking, nuclei isolation, and chromatin sonication were performed using truChIP Chromatin Shearing Kit (Covaris) according to manufacturer's instructions. ChIPmentation was performed according to the published protocol (Schmidl, C., Rendeiro, A. F., Sheffield, N. C., & Bock, C. (2015). ChIPmentation: fast, robust, low-input ChIP-seq for histones and transcription factors. Nature Methods, 12(10), 963–965. http://doi.org/10.1038/nmeth.3542). The purified libraries were multiplexed and captured on an Illumina flow cell for cluster generation. Libraries were sequenced for 50 single read cycles on HiSeq 3000  according to the manufacturer's protocols.
GEO Accession
GSM3388391