Honeybee worker larvae bisulfite sequencing
DNAs were sheared using a Bioruptor, modified by a pair of universal adapters, treated with sodium bisulfite, and then PCR amplified. The PCR products were then digested by DpnI to remove the universal adapters. The resulting DNA was used to generate single-end Illumina sequencing libraries following Illumina's library generation protocols. The libraries were sequenced on a high-throughput sequencing machine, Illumina Genome Analyzer IIx (GAIIx), following the manufacturer's instructions.