ddRAD sequencing of Odontesthes bonariensis: YC13-240
We followed the double-digest RADseq (ddRAD) protocol developed by Peterson et al. (2012) modified to use the enzymes MseI and PstI and a 350-550 bp size selection. Genomic libraries for 164 Odontesthes samples and five Basilichthys microlepidotus were prepared at the University of Puerto Rico Sequencing and Genomics Facility. Pooled samples were sequenced on a half-lane of a HiSeq 4000 at the University of Chicago Genomics Facility. Sequences were demultiplexed using ipyrad v. 0.7.2 (Eaton 2014).