GSM2752754: CTRL3; Macaca mulatta; RNA-Seq
Cells were detached with 0.25% trypsin-EDTA (Gibco). They were then centrifuged at 3000 x g for 3 min, supernatant removed, and the cell pellet resuspended in 100 µL Picopure lysis buffer (Applied Biosystems).  Lysates were stored at -80°C until shipment. 100 ng of each RNA sample were processed first using a mixture of random and oligo(dT) primers and reverse transcription to generate double stranded cDNA using the Ovation Universal RNA-Seq System (NuGen, San Carlos, CA). This was followed by cDNA fragmentation to an average of 300 bp using a Covaris-2 sonicator, and then a brief S1 nuclease digestion. After purification, the cDNA was processed further through the Ovation Universal RNA-Seq System (NuGen) for end repair, barcoding, InDA-C mediated ribosomal RNA depletion, and final library production with the addition of unique nucleotide barcodes to each library.
GEO Accession
GSM2752754