GSM3854524: Br_H3K27me3; Brassica rapa; ChIP-Seq
Two grams of young leaves per accession was cross-linked with 1% formaldehyde in a vacuum for 15 min. The formaldehyde was quenched by adding 2.5 mL of 2 M glycine, and vacuum infiltration was continued for 5 min.The extracted chromatin of these crosslinked tissue samples was sonicated to 100-300 bp. With protein G beads, the proteins of interest were pulled down by adding a specific antibody The ChIP libraries were constructed following the protocol of the NEXTflex® Rapid DNA-Seq Kit and sequenced on Illumina HiSeq X-Ten with the PE 150 method.
GEO Accession
GSM3854524