GSM1704616: 48 h DENV2 infection Total cell lysate mRNA-seq (rep 2); Homo sapiens; RNA-Seq
Pre-treatment with 180 uM cycloheximide for 30s. PBS wash. For fractionated cells, release cytosol with digitonin. Extract remaining sample with dodecyl maltoside. NEB Directional Small RNA kit PNK end polishing, linker ligation, reverse transcription, PCR amplification
GEO Accession
GSM1704616