GSM2407366: PF Amt WT, biological replicate 1; Trypanosoma brucei; MNase-Seq
MNase digestion of permeabilised cells: 5 x 107 cells/sample were harvested, permeabilised with digitonin and digested with MNase.  Mono-nucleosomal DNA (~150 bp) was gel-purified as described previously (Cole et al. 2012, PMID: 25348398). Mono-nucleosomal DNA was repaired using the NEB PreCR Repair Mix. Following Illumina protocol: T4 polynucleotide kinase was used to add a 5'-phosphate to the DNA and then Klenow enzyme was used to add a 3'dA. The Illumina paired-end adaptor was ligated to the nucleosomal DNA. Indices were added to the DNA during PCR according to Illumina kit instructions.
GEO Accession
GSM2407366