GSM3860380: NB73.3: 9hr NB73; MCF-7 cells; Homo sapiens; RNA-Seq
RNA was extracted using Trizol protocol, followed by DNase treatment.  RNA was then re-purified with an aqueous column-based protocol followed by gel analysis to analyze purity and quality of the RNA. The RNAseq libraries were prepared with Illumina's 'TruSeq Stranded mRNAseq Sample Prep kit' (Illumina). Read 1 aligns to the ANTISENSE strand and Read 2 aligns to the SENSE strand. The libraries were quantitated by qPCR and sequenced on one lane for 101 cycles from one end of the fragments on a HiSeq 4000 using a HiSeq 4000 sequencing kit version 1. Fastq files were generated and demultiplexed with with the bcl2fastq v2.17.1.14 Conversion Software (Illumina).
GEO Accession
GSM3860380