GSM1520028: shControl-3; Mus musculus; RNA-Seq
rAAV-shRNA infected regions of hippocampal tissue were identified by mCherry fluorescence, dissected under a fluorescence microscope, and stored in RNAlater (Ambion). Total RNA was isolated using a QIAGEN RNeasy kit with additional on-column DNase I digestion according to the manufacturer's instructions. Non-strand-specific RNA-seq libraries were prepared from 1.5 µg of each total RNA sample with the NEBNext RNA Library Prep Kit for Illumina, polyA mRNA was selected for the library construction.
GEO Accession
GSM1520028