GSM1891972: MI63_treated_MCF7; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNeasy mini kit (Qiagen, CA) and assayed using a 2100 Bioanalyzer (Agilent, CA). ~ 0.5-1.0 µg cDNA from each was first sheared to a size ranging between 200-300 bp using the Covaris-S2 sonicator (Covaris, Woburn, MA) according to the manufacturer’s recommended protocols, and the fragmented cDNA samples were then prepared for whole transcriptome library generation using the TruSeq v1 Multiplex Sample Preparation kit (Illumina, San Diego, CA). Briefly, cDNA fragments were end-repaired, dA-tailed and ligated to multiplex adapters according to manufacturer's instructions. After ligation, DNA fragments smaller than 150 bp were removed with AmPure XP beads (Beckman Coulter Genomics, Danvers, MA). The purified adapter ligated products were enriched using polymerase chain reaction (14 cycles). The final amplified libraries were resolved on 2.0% agarose gel and manually size-selected in the range of 350–380 bp. The final RNA-Seq libraries were quantitated using the Agilent 2100 Bioanalyzer and pooled together in equal concentration for sequencing.
GEO Accession
GSM1891972