injection of dsVIH
The Oligo(dT) beads were used to isolate poly(A) mRNA, which were then interrupted into short sections with the fragmentation buffer. The short mRNA fragments were used as templates, with random hexamer primers, to synthesize first-strand cDNA. Then, the second-strand cDNA was synthesized with dNTPs, Rnase H, DNA polymerase I, and response buffer. The double-stranded cDNAs were purified using the QiaQuick PCR kit (Qiagen, Germany) and eluted with EB buffer for end reparation and tailing A. After that, the short fragments were connected with sequencing adapters and the suitable 200-300 bp fragments were selected to perform PCR amplification for library construction. At last, the cDNA libraries were sequenced on the Illumina HiSeq2500 platform and 150 bp paired-end raw reads were generated.