Illumina HiSeq 1000 paired end sequencing; De-novo assembly of transcriptome from the eggs and early embrios of Astropecten Aranciacus
De-novo assembly of transcriptome from the eggs and early embrios of Astropecten Aranciacus
Starfish (Astropecten aranciacus) were captured in the Gulf of Naples, Italy, and were kept at 16˚C.  Oocytes at the germinal vesicle (GV) stage were isolated . To obtain mature eggs, the oocytes were exposed to 1-MA (10 µM) for 70 min in filtered  seawater. The eggs were then fertilized with sperm and allowed to undergo cleavage for 4 h and  20 min. Paired-end sequencing (100 bp × 2 non stranded) of the libraries was performed on  Illumina platform. cDNA libraries were constructed according to the Illumina Paired End mRNA library protocol
Experimental Factor: developmental stage
mature egg