GSM2221811: NC-Pre-miR RA Patient 2; Homo sapiens; RNA-Seq
total RNA was prepared using miRNeasy kit (Qiagen).Total RNA integrity was checked using an Agilent Technologies 2100 Bio analyzer 10 ng of high quality RNA was used to make cDNA for amplification with the Ion AmpliSeq Transcriptome Human Gene Expression kit (ThermoFisher Scientific).  The cDNA was subjected to 12 cycles of amplification with panel primers and barcoded with adapters as recommended. Resulting sequencing libraries were quantified by qPCR using SYBR FAST master mix from KapaBiosystems (Wilmington, MA).  Sets of eight libraries were balanced, pooled and sequencing beads produced on an Ion Chef.  Sequencing was performed on an Ion P1 semi-conductor sequencing chip using an Ion Proton™ System (ThermoFisher Scientific, Grand Island, NY).
GEO Accession
GSM2221811