Promoter Capture-C: HeLa rep 1.2
Proximity ligation 3C-seq library was prepared from 5 million HeLa cells. This library was enriched with ligation products of 861 pre-selected promoters by the means of in-solution hybridisation reaction with biotinylated dsDNA probes. Post-enrichment library was subjected to 150 bp paired-end sequencing on an Illumina HiSeq 4000 platform.