16S DNA-Seq of Amblyomma americanum: nymph bacterial community-Plate2C_TTGGAGG_2010Aamer105
Barcoded eubacterial 16S primers 357F and 926R were used to amplify variable regions 5-3 from whole tick DNA extract. Invitrogen AccuPrime Taq DNA Polymerase High Fidelity was used for primary PCR. Amplicons were sequenced on the Roche 454 FLX Titanium system. Library ID is formatted as follows: PlateID_SampleMID_SampleName.