GSM3854445: M3-761; Homo sapiens; RNA-Seq
Tissues were grinded in liquid nitrogen, total RNA was extracted using All Prep DNA/RNA/Protein Mini Kit (QIAGEN, Germany). Qubit TM RNA HS Assay Kit (Invitrogen, USA) was used for the quantitation of the total RNA. After erasing rRNA from the total RNA by KAPA Stranded RNA-Seq Kit with RiboErase (KAPA, USA), strand specific lncRNA library were constructed. The quality and quantity of the library were detected by Qubit TM dsDNA HS Assay Kit (Invitrogen, USA), the inserts of the library were detected by High Sensitivity DNA Chip using Agilent 2100 Bioanalyzer system. The molarity of the library was detected by Step One Plus™ Real-Time PCR System.
GEO Accession
GSM3854445