GSM4337749: hstp4_4_5; Mus musculus; RNA-Seq
Whole brains were isolated, cut into 8-10 pieces, and immersed overnight in RNALater at 4°C, at which point they were transferred to -80°C for storage until being processed. Frozen brain tissues were homogenized in TRIzol (ThermoFisher Scientific) using a gentleMACS dissociator (Miltenyi Biotec Inc). Total RNA was isolated by the Genome Technologies core facility at The Jackson Laboratory using the TRIzol Plus RNA Purification Kit (ThermoFisher Scientific), according to manufacturers' protocols, including an optional DNase digest step. Sample concentration and quality were assessed using the Nanodrop 2000 spectrophotometer (Thermo Scientific) and the RNA 6000 Nano LabChip assay (Agilent Technologies). Poly(A) RNA-seq libraries were constructed by the Genome Technologies core facility at The Jackson Laboratory using the TruSeq RNA Library Prep Kit v2 (Illumina). Libraries were checked for quality and concentration using the DNA 1000 LabChip assay (Agilent Technologies) and quantitative PCR (KAPA Biosystems), according to the manufacturers' instructions. Libraries were pooled and sequenced 100 bp paired-end on the HiSeq 2500 (Illumina) using TruSeq SBS Kit v3-HS (Illumina).
GEO Accession
GSM4337749