16S DNA-Seq of Amblyomma americanum: adult male bacterial community-GPZ3F3403_CGGTAT_TRM374
Barcoded eubacterial 16S primers 357F and 926R were used to amplify variable regions 5-3 from whole tick DNA extract. Invitrogen Platinum PCR SuperMix High Fidelity was used for primary PCR. Libraries from plates GPZ3F3402 and GPZ3F3403 were produced from the same PCR products. Amplicons were sequenced on the Roche 454 FLX Titanium system. Library ID is formatted as follows: PlateID_SampleMID_SampleName.