RNASeq of human HNSCC cell line: UMSCC22B: Sample UM-SCC-22B
We used SOLiD™ Total RNA-Seq Kit (Life technologies) by manufacture's protocol 4452437 Rev B. 10ug of total RNA were rRNA depleted using Illumina's Epicentre Ribo-Zero Magnetic Kit (Cat#: MRZH11124) and fragmented to the median size of 125-200bp; 100ng fragmented rRNA-depleted RNA were ligated with Solid Adaptor; reverse transcription were done with Solid RT primer; size selection with 2 runs of bead capture using Agencourt AMPure XP Reagent to remove <150bp cDNA; the cDNA were amplified and barcoded by PCR. The cDNA libraries were multiplexed together, further amplified and sequenced on the SOLiD™ 4 system following the manufacturer's protocols.
alignment_software
LifeScope v.2.5 Genomic Analysis Software