18S V7-9 amplicons Ross Sea : North Pennell Bank with no iron addition, replicate 2
Samples were prepared for Circular Consensus Sequencing using the standard Pacific Biosciences 500 bp template preparation and sequencing protocol (available at: http://www.smrtcommunity.com/SamplePrep). Sequencing was conducted using 2 × 45 min movies on a Pacific Biosciences RSII Single-Molecule Sequencer (Pacific Biosciences, Menlo Park, CA) using a C2/C2 DNA Polymerase binding kit and DNA sequencing kit 2.0 (Pacific Biosciences). Files represent raw reads directly converted from HDF5 files and demultiplexed. No quality control performed. Only 100% tolerance to barcode allowed. Filtered CCS reads.