GSM2482933: Col x Cvi rep 1_Endosperm; Arabidopsis thaliana; ncRNA-Seq
Seeds from crosses between multiple individuals were hand-dissected and total embryo or endosperm RNA isolated using the Ambion RNAqueous Micro Kit. The small RNA pool was separated from the larger RNA pool by sequential ethanol addition and filtering. The larger RNA pool was collected on filters after the addition of ethanol equivalent to 60% of the sample volume, and the small RNA pool was collected on filters after the addition of ethanol equivalent to 50% of the flowthrough volume from the larger RNA filter binding step.  Libraries for Illumina sequencing were constructed using the NEXTflex Small RNA-Seq Kit v2 as directed (Bioo Scientific Corporation, Austin, Texas). 40 base single-end sequencing of sRNA libraries was performed on an Illumina HiSeq 2000 machine.
GEO Accession
GSM2482933