GSM2907198: POLR2A_plasmid Oxfored Nanopore sequencing barcode07_T6; Homo sapiens; OTHER
The plasmids of wild-type and a series of Alu deletion constructs for the    POLR2A transcription segments (exons 9 and 10) cloned into pZW1 were    directly obtained from Prof. Ling-Ling Chen (Chinese Academy of Sciences,    China). We examine whether deletions of inverted *Alu* repeats (IR*Alu*s)    can simultaneously affect *POLR2A* tsRNA and *POLR2A* circRNA. The EV    plasmid was wild-type, and T1-T7 were seven different IR*Alu* deletion    constructs (please see our article Figure 4F). Library for Nanopore sequencing was constructed using Ligation sequencing Kit 1D (SKQ-LSK108) and Native barcoding kit (EXP-NBD103) following Oxford Nanopore Technologies (ONT) protocol.                                 barcode1: AAGAAAGTTGTCGGTGTCTTTGTG;   barcode2: TCGATTCCGTTTGTAGTCGTCTGT;  barcode3: GAGTCTTGTGTCCCAGTTACCAGG;  barcode4: TTCGGATTCTATCGTGTTTCCCTA;  barcode5: CTTGTCCAGGGTTTGTGTAACCTT;  barcode6: TTCTCGCAAAGGCAGAAAGTAGTC;  barcode 7: GTGTTACCGTGGGAATGAATCCTT; barcode 8: TTCAGGGAACAAACCAAGTTACGT Oxford Nanopore amplicon
GEO Accession
GSM2907198