High throughput amplicon sequencing fungal ITS2 rRNA region: Havana soil read 2
Amplification of fungal ITS2 rRNA region using a slightly modified two-step amplification of Lunberg et al. (2013) and fungal ITS2 rRNA primers from White et al. (1990), Tedersoo et al. (2014) and unpublished primers (Dr. Schadt Lab, ORNL) and standard amplicon sequencing library prep and sequencing on Illumina MiSeq platform.