YNOV106
Yeast DNA was prepared using MasterPureTM Yeast DNA Purification Kit (Epicentre, MPY80200) and quantified by fluorimetric assay using the Quant-iTTM PicoGreendsDNA Kit (Invitrogen, Carlsbad, CA, USA). Libraries were prepared using the Nextera XT library preparation workflow (Illumina, San Diego, CA, USA), in order to obtain DNA fragments ranging in size from 600 to 1400 bp approximately. Sequencing was carried out on the Illumina MiSeq platform generating about 8 million 250x2 paired-end reads for each sample