Illumina HiSeq 2000 sequencing; PTBP1 - Modified iCLIP
PTBP1 - Modified iCLIP
HeLa cells were crosslinked with 0.15 mJ/cm 2 254nm UV light. Following the iCLIP protocol the retrieval of protein-bound RNAs and construction of Illumina compatible libraries was done as described in Huppertz et al, 2014. The 3 prime adapter sequence is AGATCGGAAG
Experimental Factor: replicate 1
individual
Experimental Factor: antibody PTBP1
immunoprecipitate