Whole genome sequence of Ceratocystis fimbriata isolated from Punica granatum L.
Whole genome sequencing libraries were prepared with Illumina-compatible NEXTflex DNA sequencing kit (BIOO Scientific, Austin, Texas, U.S.A.). Approximately 250 ng of genomic DNA was sheared using Covaris S2 sonicator (Covaris, Woburn, Massachusetts, USA) to generate approximate fragment size distribution from 200bp to 600 bp. The fragment size distribution was checked on Agilent 2200 TapeStation with D1000 DNA screen tapes and reagents (Agilent Technologies, Palo Alto, CA, USA)and subsequently purified using HighPrep magnetic beads (Magbio Genomics Inc, USA). The purified fragments were end-repaired, adenylated and ligated to Illumina multiplex barcode universal adaptors as per NEXTFlex DNA sequencing kit protocol. The adapter-ligated DNA was purified with HighPrep beads and then amplified for 10 cycles of PCR using Illumina-compatible primers provided in the NEXTFlex DNA sequencing kit. The final PCR product (sequencing library) was purified with HighPrep beads, followed by library quality control check. The Illumina-compatible sequencing library was initially quantified by Qubit fluorometer (Thermo Fisher Scientific, MA, USA) and its fragment size distribution was analyzed on Agilent TapeStation. Finally, the sequencing library was accurately quantified by quantitative PCR using Kapa Library Quantification Kit (Kapa Biosystems, Wilmington, MA, USA). TheqPCR quantified library was subjected to sequencing on an Illumina sequencer for 150 bp paired-endchemistry.