GSM2221341: CHiC E12.5 Dup-S; Mus musculus; OTHER
Limbs were re-suspended in Trypsin and incubated for 10 minutes at 37°C. Tissues were disrupted by pipetting and single cells were retrieved using cell strainer and fixed for 10 minutes at room temperature in 10ml (2%PFA, 10%FCS, 1XPBS). Final 140mM glycine was added to stop crosslinking. Cells were then lysed for 10' at 4°C in  lysis buffer (50mM TRIS pH7.5, 150mM NaCl, 5mM EDTA, 0.5%NP-40, 1.15% TX-100 and 1X Proteinase Inhibitor). Sheared DNA was then ligated to adaptors and 4 amplification cycles were performed prior to SureSelect hybridization. 700ng of adaptor-ligated DNA was hybridized to the custom probes according to Agilent instructions. Hybridized fragments were then amplified (12 cycles) and products were cleaned using AMPure XP beads. Each sample was sequence on an individual Illumina HiSeq lane following a 100bp paired-end mode.
GEO Accession
GSM2221341