BGISEQ-500 paired end sequencing; RNA-Seq of enteroids from wild-type and Tnfaip8 knockout mice.
RNA-Seq of enteroids from wild-type and Tnfaip8 knockout mice.
RNAs were collected from MDSC using RNAeasy kit. rRNA depletion or Oligo dT Selection to enrich the mRNA RNA fragment and reverse transcription, End repair, add A and adaptor ligation, RCR amplification, Single strand separation and cyclization, DNA nanoball synthesis.
Experimental Factor: genotype
wild type genotype