GSM1704123: N-54; Homo sapiens; miRNA-Seq
Total RNA was isolated from serum of patients after liver allograft transplantation and used for miRNA-sequencing and qPCR analysis. The small RNA libraries for the solid tissue samples were prepared using the protocol described in Hafner et al. (Methods, 2008; Methods, 2012) and Akat et al. (PNAS, 2014) with modifications: 1) The libraries were spiked  with 2 cocktails comprising each 10 synthetic 22-nt oligoribonucleotides; the amount of each oligoribonucleotide was 1 amol, 10 amol total for each cocktail, 20 amol total in each sample. 2) The synthetic P32-labeled 19-nt and 24-nt ssRNAs used for size selection were not spiked-into the samples. The sequences for the 20 calibrator oligoribonucleotides are in the supplementary information of this publication. Directional RNA-seq (size-fractionation for RNAs 19- to 24-nt)
GEO Accession
GSM1704123