GSM3386089: mRNA_WT2; Drosophila melanogaster; RNA-Seq
Mutant and wildtype ovary samples were dissected in ice cold Grace Medium for RNA isolation. The GFP-positive GSCs and GFP-negative SCs were purified by sorting dissociated single cells at 20 psi with 100 um tip (BD, InFlux) immediately into TRIzol LS Reagent (Thermo Fisher Scientific, 10296028) for total RNA isolation. Standard Illumina TruSeq Stranded mRNA-seq or smallRNA-seq.  For FACS sorted cells, prior to RNA isolation, spike-in RNA standards from ExiSEQ NGS spike-in kit (ExiSEQ, 800100) were added to the samples.
GEO Accession
GSM3386089