small RNAs MA line 59
Approximately 200 mg of finely ground tissue from single rosette plants containing 10 leaves was used for small RNA isolation following the instructions provided within the Ambion mirVana miRNA Isolation Kit (Ambion, Austin, TX). Small RNAs isolated from this kit were EtOH precipitated and loaded into a 15% TBE-UREA gel (Life Technologies, Carlsbad, CA). smRNAs were excised from the gel from the range of ~15-50 nucleotides in length. These smRNAs were used for library construction following the protocol provided in the TruSeq Small RNA Sample Preparation Kit (Illumina).