TW_DX_001
DNA extraction was performed using the Qiagen MagAttract PowerSoil DNA kit, in accordance with the Earth Microbiome Project (EMP) standard protocol. After extraction, DNA was quantified using the Quant-iT PicoGreen dsDNA assay (Thermo Fisher). DNA sequencing libraries were prepared using one-quarter-scale NexteraXT reactions (Illumina). The resulting DNA libraries were denatured with NaOH, diluted to 8 pM in Illuminas HT1 buffer, and spiked with 1% PhiX, was used to sequence samples. Shotgun DNA sequencing was performed on the Illumina miSeq platform._