GSM1621292: Wnt6_InvF_mm9; Mus musculus; OTHER
4C-seq libraries were generated from microdissected tissues or cells as described previously (van de Werken et al., 2012). BglII or HindIII (6-bp cutters) were used as primary restriction enzymes. Csp6I or DpnII were used as secondary restriction enzymes. For each viewpoint, a total of 1.6 mg of each library was amplified in 8 or 16 PCR reactions, depending on PCR efficiencies. Samples were multiplexed and sequenced with Ilumina Hi-Seq technology. The following primers were used: Wnt6_promoter_forward: CTACACGACGCTCTTCCGATCTCCACAGATGCCTACATCAG, Wnt6_promoter_reverse: CAGACGTGTGCTCTTCCGATCTCAGTTGGACAGCCTTCTACC,  Ihh promoter_forward: CTACACGACGCTCTTCCGATCTCTTGCTCGGGATTCAGAT, Ihh_promoter_reverse: CAGACGTGTGCTCTTCCGATCTGCATGTCTAGCATGGTGGTA, mm1036_forward: CTACACGACGCTCTTCCGATCTACCTTATCTTGGCCTTCAA,  mm1036_reverse: CAGACGTGTGCTCTTCCGATCTCCATAACCCAGACTGAGATG, Epha4_promoter_forward: CTACACGACGCTCTTCCGATCTTGGCTTTTACCGAAAATAAA, Epha4_promoter_reverse: CAGACGTGTGCTCTTCCGATCTTCGTAGTCTAGGTGGAGGAA Pax3_promoter_forward: CTACACGACGCTCTTCCGATCTTTCTATCTCCCCATGACACC, Pax3_promoter_reverse: CAGACGTGTGCTCTTCCGATCTTCCTCCAAACGTGCTCTG Samples were multiplexed and sequenced with Ilumina Hi-Seq technology according to the manufacturer´s protocol.
GEO Accession
GSM1621292