Ls_1x10e9_O157H7_24HPI_BR2.2
Libraries were prepared using both Ribo-Zero rRNA Removal Kits, Bacteria and Plant (Illumina, CA, USA). Completed libraries were quality controlled and quantified using a combination of Qubit dsDNA HS and Advanced Analytical Fragment Analyzer High Sensitivity NGS DNA assays. Pools were quantified using the Kapa Biosystems Illumina Library Quantification qPCR kit. Sequencing was conducted using the Illumina HiSeq 4000 flow cell and performed in a 1x50 bp single read format using HiSeq 4000 SBS reagents. Base calling was done by Illumina Real Time Analysis (RTA) v2.7.7 and the RTA output was demultiplexed and converted to FastQ format with Illumina Bcl2fastq v2.19.1.