GSM1621086: S2 repB light digest 4 h; Drosophila melanogaster; MNase-Seq
MNase-digested DNA samples were resolved on 2% agarose gel. DNA band containing mononucleosomal DNA (<=200bps) was excised and purified by the freeze-squeeze method. A mononucleosomal DNA library was prepared using NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (NEB #E7370S/L) according to manufacturer’s instructions. Each reaction used 30ng of mononucleosomal DNA. After end prep, adaptor ligation and indexing (NEB #E7335S/L, NEB #E7500S/L), the samples were cleaned with AMPure XP beads (Beckman Coulter, Inc. #A63881) without size selection. The library was amplified by 10 cycles of PCR, and again cleaned with AMPure XP beads. The quantity and quality of the library were checked with Qubit 2.0 Fluorometer (Life Sciences) and Agilent 2100 Bioanalyzer (Agilent Technologies). Paired-end 50bps sequencing was performed using cBOT cluster generation in Illumina HiSeq 2500.
GEO Accession
GSM1621086