GSM3611555: ATAC_sp7ne0dpa_rep1; Danio rerio; ATAC-seq
70,000 cells were washed with 50 µL of cold PBS buffer, lysed in 50 µL of cold lysis buffer (10 mM Tris, pH 7.4, 10 mM NaCl, 3 mM MgCl2 and 0.1% IGEPAL CA-630), and incubated with TDE1 enzyme (from Nextera DNA Sample Preparation Kit, Illumina) for 30 minutes at 37 ºC for transposition. Transposed DNA fragments were immediately purified by using MinElute PCR Purification Kit (Qiagen) ATAC-seq libraries were amplified by using eleven cycles of PCR amplification with the initial 5-minute extension at 72 ºC, and purified by using the AMPure XP Beads (Beckrman Coulter). The purified libraries were eluted with 20 µL of nuclease-free water.
GEO Accession
GSM3611555