GSM4673397: C5: Human Normal Fibroblast 17517; Homo sapiens; RNA-Seq
Total RNA from cultures C1,C2, P1 to P16 was extracted with RNeasy Mini and Micro kits (Qiagen) and total RNA from cultures C3 to C8 was extracted with NucleoSpin RNA plus kit (Macherey – Nagel), followed by quality control using capillary electrophoresis (RNA 6000 Nano chips, Agilent). Libraries were prepared according to Illumina's instructions with theTruSeq RNA Library Prep Kit v2. Briefly, total RNA (1µg) was purified and fragmented before first strand and second strand cDNA synthesis. Then ends are repaired and 3'ends adenylated before adapters ligation and PCR amplification. Libraries were then validated, normalized, and sequenced on Illumina HiSeq 1000 as short-insert paired-end libraries with read lengths 100 bp.
GEO Accession
GSM4673397