GSM1703608: RNA PolII_ChIPseq; Homo sapiens; ChIP-Seq
Lysates were clarified from sonicated nuclei and DNA complexes were isolated with antibody. ChIP and Input DNAs were prepared for amplification by converting overhangs into phosphorylated blunt ends and adding an adenine to the  3 -ends. Illumina genomic adapters were ligated and the sample was  size-fractionated (200-300 bp) on a 2% agarose gel. After a final PCR  amplification step (18 cycles), the resulting DNA libraries were  quantified and sequenced on HiSeq 2000.
GEO Accession
GSM1703608