GSM3387232: at-endosperm-RNAseq_replicate1_Landsberg_wt_x_Columbia_wt; Arabidopsis thaliana; RNA-Seq
For RNA sequencing, endosperm nuclei from seeds derived from indicated crosses were isolated using the INTACT protocol using replicates as indicated. RNA was extracted following a mirVana kit (Ambion). mRNA extraction was performed using NEBNext Poly(A) mRNA Magnetic Isolation kit. Samples were sequenced at the National Genomic Infrastructure (NGI) from SciLife Laboratory (Uppsala, Sweden) on an Illumina HiSeq2500 in paired-end 125bp read length.
GEO Accession
GSM3387232