RNA-seq of Schistocerca nitens: isolated-reared L6 nymph thorax tissue, biological replicate 4
Library preparation was performed using Illuminas TruSeq Stranded Total RNA Library Prep Kit, followed by paired-end sequencing (150 bp) using 8.5 lanes on an Illumina HiSeq4000. Cluster identification, quality prefiltering, base calling and uncertainty assessment happened in real time with Illumina's software (HCS 2.2.68; RTA 1.18.66.3; default parameter settings). Demultiplexing base call files and formatting them into FASTQ files was carried out using Illuminas bcl2fastq script (version 2.17.1.14). All steps were performed at Texas A&Ms AgriLife Research Genomics and Bioinformatics Service.