GSM2406694: ATACseq doxcycline rep1; Homo sapiens; ATAC-seq
50,000 cells were lysed for nuclei and was incubated with transposase reaction mix for 30 min at 37 degrees. Libraries were prepared according to Nextera DNA Sample Prep kit. Briefly, library fragments were amplified using 1x NEBnext PCR master mix. Libraries were purified using Qiagen PCR cleanip kit. Libraries were amplified for a total of 13 cycles. Libraries were selected for 240-360bp size fragments.
GEO Accession
GSM2406694