Pseudomonas aeruginosa. Population 12. Control. Evolved
Genomic DNA was extracted at the end of the evolution assays from all 12 populations using the Gnome DNA kit (MP Biomedicals). Whole-genome sequencing was performed by Sistemas Genmicos S.L. Libraries were obtained without amplification following Illumina protocols and recommendations. The quality of the extracted material was analyzed via a 4200 Tapestation High Sensitivity assay, and the DNA concentration determined by real-time PCR using a LightCycler 480 device (Roche).