"ONT sequecing of yeast W303, sample  DAVE_titration_5ng_081414_R7"
"R7.0 DNA was sheared following Covaris instructions 10kb, 2ug DNA input then split into two equal aliquots prior to ligation, adapter mix allowed to incubate for 5 minutes with ligase prior to adding HP adapter, 30min motor incubation, 5ng DNA estimated added to flow cell, otherwsie standard ONT R7.0 prtocol  "