GSM3386161: Liver rep2; Pimephales promelas; RNA-Seq
Tissue extractions followed procedures previously described (Garcia-Reyero et al., 2009; Bisesi et al., 2017).  Briefly, tissues were homogenized in RNA Stat-60 (TelTest) using a handheld rotary homogenizer followed by organic separation with chloroform. RNA was then subjected to a second round of RNA Stat-60/Chloroform extraction, followed by precipitation in isopropanol overnight at -20°C.  RNA was washed twice with 75% ethanol, dried, and reconstituted in RNAsecure (ThermoFisher Scientific). Reconstituted RNA was DNase-treated to remove possible genomic DNA contamination using Turbo DNase (ThermoFisher Scientific). The quality of the RNA was assessed using an Agilent Bioanalyzer 2100.  Only samples with RNA integrity numbers (RINs) exceeding 8 were used for sequencing.  Samples were then quantified using a ThermoFisher Scientific Qubit 3.0 fluorimeter.
GEO Accession
GSM3386161