GSM1945848: midlife-chm 1; Drosophila melanogaster; RNA-Seq
Total RNA was prepared from 25-30 fly heads using 0.5 ml Qiazol (Qiagen) according to manufacturer’s instructions. 2.5 µg of RNA were used for polyA selection and subsequent RNA purification was achieved with RNeasy mini columns (Qiagen). Libraries were prepared using New England Biolabs reagents according to protocols (E7490, E6150, E7525, E6111, E7442, E7335). The ligation was done at 16°C overnight with regular ligase (M0202) and High Fidelity PCR mix (M0541) was used for amplification. Quantifications and quality controls were done with Qubit RNA HS detection kit (Life Technologies) and the RNA 6000 pico kit and DNA 2000 kits (Agilent). 50 bp single reads were obtained on an Illumina Hiseq sequencer.
GEO Accession
GSM1945848