GSM2752872: Control 2; Homo sapiens; Bisulfite-Seq
Extraction of DNA performed with Blood and Tissue extraction kit (Qiagen) according to the manufacturer's instructions. Libraries were prepared from 200-500 ng of genomic DNA digested with 60 units of TaqαI and 30 units of MspI (NEB) sequentially and then extracted with Zymo Research (ZR) DNA Clean  & Concentrator™-5 kit. Fragments were ligated to pre-annealed adapters containing 5’-methyl-cytosine instead of cytosine according to Illumina’s specified guidelines (www.illumina.com). Adaptor-ligated fragments of 150–250 bp and 250–350 bp in size were recovered from a 2.5% NuSieve 1:1 agarose gel (Zymoclean™ Gel DNA Recovery Kit, ZR). The fragments were then bisulfite-treated using the EZ DNA Methylation-Lightning™ Kit (ZR). Preparative-scale PCR was performed and the resulting products were purified (DNA Clean & Concentrator™ - ZR) for sequencing on an Illumina HiSeq.
GEO Accession
GSM2752872