GSM4561502: B73_seedling_leaf_rep_6; Zea mays; Bisulfite-Seq
DNA was extracted from leaves of two week old V2 glasshouse grown maize B73 plants using the DNeasy Plant Mini kit (Qiagen). 1ug of DNA in 50ug of water was sheared using an Ultrasonicator (Covaris) to approximately 200-350 bp fragments. 20ul of sheared DNA was then bisulfite converted using the EZ DNA Methylation-Lightning Kit (Zymo Research) as per the manufacturer's instructions and eluted in a final volume of 15ul. Then 7.5ul of the fragmented, bisulfite-converted sample was used as input for library preparation using the ACCEL-NGS Methyl-Seq DNA Library Kit (SWIFT Biosciences). Library preparation was performed as per the manufacturer's instructions except each reaction was scaled by a half. The indexing PCR was performed for 5 cycles. Libraries were then pooled and sequenced on a HiSeq 2500 in high output mode 125bp paired end reads over multiple lanes at the University of Minnesota Genomics Centre.
GEO Accession
GSM4561502