PAR-CLIP of hnRNPC in Homo sapiens HEK293
PAR-CLIP libraries were prepared according to a modified version of the         protocol as described in: Martin G et al. 2012. Genome-wide analysis of pre-mRNA 3’ end         processing reveals a decisive role of human cleavage factor I in the regulation of 3' UTR         length. Cell Rep 1: 753–63. Compared to the original protocol, preblocking of         Dynabeads-protein-A (Life Technologies) was included as an additional step resulting in         reduced background and higher efficiency of library generation.