Genome resequencing of Dular rice
Genomic DNA (gDNA) was extracted from leaf tissue using Qiagen DNeasy kit (Qiagen USA) as per Manufacturerís instructions. Integrity of gDNA was assessed by Bioanalyzer 2100 (Agilent Technologies, Singapore). gDNA sample preparation for sequencing was done using Illumina TruSeq DNA sample preparation kit (Illumina, USA). Briefly, one µg high quality gDNA was subjected to fragmentation by Covaris shearing to obtain a final library of 300 to 400 bp average insert size.  Sheared DNA was then subjected to end repairing, adenylation of 3í ends followed by ligation of indexed paired end adapters. Ligated products falling in size range of 400-500 bp were purified using Qiagen MinElute gel extraction kit and selectively enriched by PCR. The libraries so generated were validated for right fragment size, concentration and pooled as per manufacturesí instructions. Finally, libraries were sequenced by Illumina HiSeq 2000 NGS platform