mRNA-Seq for homoeologous transcript quantitation in polyploid Brassica napus
The Illumina mRNA-Seq kit (RS-100-0801, Illumina Inc.) was used according to the manufacturers protocol to convert total RNA into a library of template molecules suitable for high throughput DNA sequencing for subsequent cluster generation. Libraries were prepared using 5 micrograms of total RNA, quantification and quality assessment were carried out by running 1 microlitre of library on an Agilent DNA 1000 LabChip (Agilent Technology 2100 Bioanalyzer).