16S rRNA gene amplicons of sus scrofa: Ear
16S rRNA gene amplicons were prepared using primers 16SForw 5'-AGAGTTTGATCCTGGCTCAG-3' and 16SRev 5'-CTGCTGCCTYCCGTA-3'. The PCR program included 94°C for 6 min and 30 cycles of 94°C for 45 s, 57°C for 45 s, 72°C for 90 s, and 72°C for 10 min (final extension).