Molluscum Contagiosum Virus Transcriptome in Abortively Infected Cultured Cells and Human Skin Lesion: Sample 12hpi+CHX
Trizol Reagent (Ambion 15596-018) was used for RNA purification following the manufacturer’s protocol. RNA was extracted with phenol:chloroform:isoamyl alcoho (1:1:25), ethanol precipitated and suspended in water. Polyadenylated RNA was captured on solid-phase magnetic beads (Life technologies 61006) and RNA libraries were prepared using ScriptSeq v2 kit (Epicentre SSV21106). For RNA extracted from cells infected at 31o 150 C, a ribosomal RNA removal step was included following the manufacturer’s protocol (Epicentre MRZH116) before poly(A) selection.