GSM4065244: AC16_28d24h37d2h_C2; Homo sapiens; RNA-Seq
Subcellular fractionation into nuclear and cytoplasmic fractions followed by RNA isolation using Triazol reagent (Neve et al., 2015). For spiked in samples, cells were spiked with a known ratio of D. melanogaster S2 cells prior to subcellular fractionation. The 3' ends of extracted RNA were generated into libraries using the QuantSeq 3'mRNA-Seq library kit (Lexogen). Full length RNA libraries were generated using the using the Ion Total RNA-seq Kit v2 (ThermoFisher). For this full-length RNA-seq polyadenylated RNAs were first purified from total RNA from each fraction using the NEBNext Poly(A) mRNA Isolation Module.
GEO Accession
GSM4065244