GSM4064958: D4lo ATAC-seq; Homo sapiens; ATAC-seq
Cells were stained with surface markers and CaspGLOW kit (eBioscience) and sorted at day 4 as D4lo cells and at day 5 as P2/CD23+ (VPDlo/CD38lo/CD23+), P2/CD23- VPDlo/CD38lo/CD23) and P1 (VPDlo/CD38hi) cells using a FACsARIA cell sorter (BD Biosciences). 50,000 live cells were manually counted immediately after sorting and ATAC-seq libraries were constructed according to the original ATAC-seq protocol (Buenrostro et al., 2013) with no further modification. No size selection was performed to keep a complete view the the open chromatin landscape. Quantity and quality of libraires were assessed by BioAnalyzer (Agilent) and qPCR (Kapa) analyses. Libraries were sequenced as a single equimolar pool on one lane of a paired end 2x100 bp run on the Illumina HiSeq4000 sequencer by an external provider (Integragen, Evry, France).
GEO Accession
GSM4064958