GSM3611433: imb_koenig_2018_12_sutandy_RNAseq_U2AF65mut_06_WT_03; Homo sapiens; RNA-Seq
Hela was lysed in 1ml lysis buffer followed by immunoprecipitation as described on previously published protocol (Huppertz et al., 2014; Sutandy et al., 2016) in vivo iCLIP libraries were prepared from HeLa cells under U2AF65 KD and wild-type conditions according to the previously published protocol (Huppertz et al., 2014; Sutandy et al., 2016). For immunoprecipitation, we used 7.5 µg monoclonal anti-U2AF65 antibody produced in mouse (Sigma cat. no. U4758) per sample. Each iCLIP library was done in triplicates. The libraries were sequenced as single-end reads on an Illumina HiSeq 2500 and NextSeq 500 sequencing system.