Ssc_Shrt_NRML_KKK_2013
Sequence libraries were constructed were performed following the Applied Biosystems/Life Technologies protocols. The short RNA fragments were size fractionated from total RNA following ligation ligation of adaptor primers and 60-80 nt fragments were isolated. With the adaptors, these fragments equated to 10-40 nt short RNA fragments. The adaptor sequence used was '330201030313112312' (sequence is in color space). Emulsion PCR and sequencing was performed on the SOLiD platform following life technologies protocols.