Whole Exome Sequencing of squamous cell carcinoma samples
For sequencing, approximately 1.0 micrograms of genomic DNA from tumor and normal tissue was sheared by sonication to a target length of 200 bp.  About 40 megabases of coding sequence were targeted using oligonucleotide-based hybrid capture using Agilent SureSelect Exome Capture kits.  Sequencing-by-synthesis using the Illumina GAIIx system resulted in more than 85% of targeted regions receiving 14x fold coverage at >90% of bases.