GSM4337068: T_2_RNA-seq; Mus musculus; RNA-Seq
Subcutaneous tumors were extracted from euthenized mice and mechanically dissociated, washed with PBS then strained through a 70 um filter. Immune cells were enriched using Percoll gradient centrifugation. Cells were stained and isolated using a FACS Aria machine. Cells were then processed for RNA isolation using the Norgen BioTek Total RNA Purification Plus Micro Kit. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM4337068