Targeted-Capture of Rubus subgenus Rubus:Low brix :Oregon:Leaf
Genes associated with fruit  sugar production in blackberry were identified using a family-level candidate  gene approach. Rubus transcripts were identified from the Rubus reference  transcriptome (https://www.rosaceae.org/analysis/227) using sugar candidate  genes from apple (Malus domestica), peach (Prunus persica), and alpine  strawberry (Fragaria vesca). These genes were used to design Hyb-Seq baits at  a 2X tiling density. The baits were used in conjunction with PacBio  sequencing to genotype 40 cultivars with high and low soluble solids content  (SSC) from the University of Arkansas and USDA blackberry breeding programs.  Sequencing and filtering of the captured genomic reads for the 40 samples  produced circular consensus (CCS) reads.