GSM4497128: Infection_1; Homo sapiens; RNA-Seq
Blood samples were collected from SFTS-suspected patients between 2014 and 2019. All work with human subjects was approved by the Chungbuk National University Hospital (IRB approval number: 2017-05-002-001) and Asan Hospital (IRB approval number: 2016-0046). PBMCs were extracted using Ficoll-Paque PLUS (GE Healthcare, Uppsala Sweden), and stored at - 80 ºC. PBMC from each individual were processed separately through the ChromiumTM Single Cell Platform with the 10X Genomics ChromiumTM Single Cell 3' Library and Gel Bead Kit v2 and the Chromium Single Cell A Chip Kit (PN-120267) using the standard manufacturer's protocol. The constructed libraries were sequenced on Hiseq 4000 (Illumina, San Diego, CA) system in a 2 x 150 paired-end with average of 50,000 reads per cell. single-cell RNA-seq
GEO Accession
GSM4497128