GSM2830205: Control Morphant TCDD_biorep_1; Danio rerio; RNA-Seq
Total RNA was extracted from 48 hpf whole embryos using RNAzol (Molecular Research Center, Inc; Cincinnati, OH) and a bullet blender with 0.5 mM zirconium oxide beads (Next Advance, Averill Park, New York) as recommended by the manufacturer. The RNA was purified using the Direct-zol MiniPrep kit (Zymo Research; Irvine, CA) and included an in-column DNase 1 digestion.  RNA quality and quantity was assessed using a SynergyMix microplate reader with the Gen5 Take3 module. RNA integrity was confirmed (RIN score > 8) with an Agilent Bioanalyzer 2100. Total RNA samples were sent to Oregon State University Center for Genome Research and Biocomputing Core facilities for library preparation and sequencing. mRNA was poly-A selected, and libraries were prepared with the PrepXTM mRNA and Illumina* sequencing workflow (Wafergen Biosystems, Fremont, CA). Paired-end sequencing (150 bp) was conducted with an Illumina HiSeq 3000 sequencer.
GEO Accession
GSM2830205