AB 3730 Genetic Analyzer sequencing; Simple sequence repeat identification for RBOHB gene in multiple varieties of Oryza sativa
Simple sequence repeat identification for RBOHB gene in multiple varieties of Oryza sativa
A leaf punch of approximately 1 centimeter in diameter was collected from plants DNA Leaf tissue from each rice variety was extracted using a Qiagen DNeasy kit per manufacturer's protocols. DNA from each rice variety was added to Promega PCR Master Mix as per manufacturer's protocol for a 50 microliter reaction.   Purification of PCR product was obtained by precipitating with a 9:1 ratio of 3M sodium acetate and 1:3 ratio of 100% ethanol. After 20 minute incubation at -20 Celsius, samples were centrifuged for 30 minutes. Ethanol was removed from the sample and the pellet containing purified PCR product was dried. PCR product was resuspended in double distilled water.
Experimental Factor: VB229
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