Pool of Globodera pallida Saint Malo population on the potato cultivar Désirée (replicate 2)
DNA was extracted directly from each pool using Qiagen DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions. Extracted genomic DNA was quantified using a Qubit® 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and quality was estimated using a Nanodrop® ND-2000 Spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA). The DNA samples conformed to the required purity criteria (A260/A230 and A260/A280 > 1.8) for gDNA library preparation for sequencing. Paired-end libraries were therefore constructed from the totality of DNA  contained in 100 µL AE buffer (Qiagen), using the TruSeq Nano DNA Sample Preparation Kits (Illumina, San Diego, CA, USA), according to the manufacturer’s instructions. After a double-purification, libraries were enriched by eight PCR cycles, followed by a final purification. Libraries were then quantified by qPCR on ABI7900HT (Applied Biosystems) in order to pool them in equimolar proportions. Sequencing was performed on an Illumina HiSeq™ 2500 platform. Libraries preparation and sequencing were performed at the GeT-PlaGe France Genomics sequencing platform (Toulouse, France).