GSM4065824: MEhigh_TNFa2_S5: MES MGT1mV/PNGT2mCh TNFa mVenus high (7.5.18); Homo sapiens; RNA-Seq
The total RNA was extracted by lysing the cell pellets in AL buffer (Qiagen), supplemented with Proteinase K (Invitrogen) and DNAse I (Thermo Scientific), subsequently purified with AMPure beads and eluted in EB buffer (Qiagen). RNA-seq libraries where constructed from 1μg of RNA following the standard Illumina TruSeq Stranded Total RNA protocol
GEO Accession
GSM4065824