Neurospora crassa conidiation time course mycelium at time 3
RNA was isolated from mycelium grown in Vogel's minimal medium culture grown at 25 degrees C was harvested on filter paper using a Buchner funnel.  The mycelial pad on filter paper was placed on Vogel's minimal agar medium for 3 hours. At that time, young aerial hyphae were visible.  This mycelium was removed with forceps and frozen immediately in liquid nitrogen for RNA extractoin.  RNA was purified and cDNA constructed using the Illumina mRNA seq prep kit and sequenced on a GA II sequencer.