GSM3611609: REU4502A5 (RN070); Rattus norvegicus; RNA-Seq
RNA was extracted from hippocampus tissue using TRI reagent as per manufacturer's instructions. The resulting RNA pellets were dissolved in 50 μl of nuclease-free water (Ambion). Purified RNA was treated with DNase I (Qiagen, Germany) and incubated for 10 min at room temperature. DNase was inactivated by the addition of EDTA (Final conc. 5mM) for 10 min at 70°C and RNA was purified over MinElute spin column as per manufacturer's instructions (Qiagen, Germany). Total RNA was depleted of ribosomal RNA using Ribo-Zero rRNA Removal Kit (Epicentre/Illumina, Human/Mouse/Rat). Library preparation was completed using TruSeq Stranded Total RNA kit (Illumina).
GEO Accession
GSM3611609