GSM1621055: CB-9_L007; Mus musculus; RNA-Seq
Control (C57BL/6J) and B6.D2 congenic mice at about 50 days of age were decaptitated for brain retrieval. As mentioned before, 3mm strialtal punches (left and right hemispheres) were pooled per mouse, and collected tissue was immediately places into RNAlater (QIAGEN) for 24 hours. After incubation, RNA was extracted via miRNeasy Mini Kit and quality was assessed by Bioanalyzer analysis. RNA that was deemed high quality was send to the University of Chicago Genomics Core Facility for cDNA library preparation. Libraries were prepared according to Illumina's detailed instructions accompanying the TruSeq® Stranded mRNA LT Kit  (oligo-dT; 50bp single-end reads; Part# RS-122-2101).  The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the HiSeq2500 following the manufacturer's protocols
GEO Accession
GSM1621055