Illumina HiSeq 2000 sequencing; Glucocorticoid receptor profiling in IMR90, K562 and U2OS (ChIP-exo)
Glucocorticoid receptor profiling in IMR90, K562 and U2OS (ChIP-exo)
For ChIP-exo experiments, approximately 15 million cells were treated with dexamethasone and chromatin was sheared essentially as described for ChIP-seq experiment.  The resulting sheared and cross-linked chromatin along with GR-antibody (N499) was sent to Peconic (PA, USA) for further processing.
Experimental Factor: cell line
K562
LIBRARY_STRAND
not applicable