Genome wide miRNA sequencing of SH-SY5Y cells overexpressing wild type MIR204 (clone 5)
SH-SY5Y cells stably overexpressing wild type MIR204 (generated via lentiviral transduction). The paternal transduced cell line was split and clones were cultured separately to give biological replicates. Sequenced library: MIR204WT cells, clone 5. Libraries were prepared using the TruSeq small RNA library kit (Illumina). PCR products were separated on a Pippin Prep system (Sage Science) to recover the 147 nt and 157 nt fractions containing mature miRNAs. Small RNA libraries were sequenced on a NextSeq 500 (Illumina) using single-end sequencing with read length of 75 bp. Library preparation and sequencing was performed at Biogazelle N.V. (Belgium).