GSM3855434: LHX4::GFP-; Mus musculus; RNA-Seq
Live cells were collected after dissociation and flow sorting for GFP signal. 3 Samples were extracted from 2 litters of E14.5 LHX4::GFP mice, pooled into one sorting solution during dissociation. For each sample (GFP+ and GFP-), approx. 4000 cells were individually lysed, and their RNA transcribed and sequenced by the Columbia University Single Cell Analysis Core. They were prepared using the 10X Chromium Single Cell 3' Reagent Kits v2 according to manufacturer's instructions (cDNA amplification cycles = 12, library amplification cycles = 12).
GEO Accession
GSM3855434