GSM3854515: JH32_AireKO_retina_scRNAseq_R2; Mus musculus; RNA-Seq
Dissociated single cells were processed through the GemCode Single Cell Platform using the GemCode Gel Bead, Chip, and Library Kits (10X Genomics, Pleasanton) as per the manufacturer's protocol.  In brief, single cells were sorted into 0.4% BSA–DPBS.  Approximately 14,000 single retinal cells were added to a channel.  The cells were then partitioned into Gel Beads in Emulsion in the 10x Genomics Chromium Controller, where cell lysis occurred Cell lysis was followed by barcoded oligo-DT priming and reverse transcription of poly-adenylated RNA, amplification, shearing and 5′ adaptor and sample index attachment.
GEO Accession
GSM3854515