GSM2831037: HFD_2_muscle; Mus musculus; RNA-Seq
RNA was isolated from mice skeletal muscle (3 wt, 3 HFD and 3 HFD + AA6) using the miRNeasy micro Kit (Qiagen) combined with on-column DNase digestion (DNase-Free DNase Set, Qiagen) to avoid contamination by genomic DNA. For next generation sequencing (NGS) RNA was isolated from mice skeletal muscle (3 control, 3 HFD and 3 HFD + AA6) using the miRNeasy micro Kit (Qiagen) combined with on-column DNase digestion (DNase-Free DNase Set, Qiagen) to avoid contamination by genomic DNA. RNA and libraries integrity were verified with a BioAnalyzer 2100 (Agilent) or LabChip Gx Touch 24 (Perkin Elmer). 1 µg total RNA was used as input for Truseq Stranded mRNA Library preparation following the low sample protocol (Illumina). Sequencing was performed on the NextSeq500 instrument (Illumina) using v2 chemistry.
GEO Accession
GSM2831037