1,4-dioxane degradation: soil F control 2
Libraries were prepared (attempted) using the Takara SMARTer ThruPLEX DNA Library Preparation Kit following manufacturer's recommendations. Completed libraries were QCd and quantified using a combination of Qubit dsDNA HS and Agilent 4200 TapeStation HS DNA1000 assays. It was decided that it would be good to proceed with sequencing for just the 18 good libraries; these were pooled in equimolar amounts for multiplexed sequencing. The pool was quantified using the Kapa Biosystems Illumina Library Quantification qPCR kit and loaded onto one (1) lane of an Illumina HiSeq 4000 flow cell. Sequencing was performed in a 2x150bp paired end format using HiSeq 4000 SBS reagents. Base calling was done by Illumina Real Time Analysis (RTA) v2.7.7 and output of RTA was demultiplexed and converted to FastQ format with Illumina Bcl2fastq v2.19.1.