GSM1703648: AND CD4TCell H3K4me2 0.1uM PCC 24h Five Conditions; Mus musculus; ChIP-Seq
Quoted from GSE62826: "Sequencing libraries were prepared from collected DNA by blunting, A-tailing, adapter ligation as previously described (Heinz, S. et al. Molecular Cell 38, 576-589 (2010)) using barcoded adapters (NextFlex, Bioo Scientific). Libraries were PCR-amplified for 12-15 cycles, size selected by gel extraction and sequenced on a Hi-Seq 2000 (Illumina) for 51 cycles." CD4+ cells were re-isolated from the culture using the Miltenyi MACS system.
GEO Accession
GSM1703648