Sample_2
The Nextera XT DNA Sample Preparation Kit was used according to the Illumina (San Diego, CA) instructions except that 16 (instead of 12) PCR enrichment cycles were used. For samples with less than 3 ng total DNA in 50 µL of non-purified extracts, the volume of Nextera tagmentase was reduced from 5 to 3 µL. The libraries were sequenced in Rapid Run mode for 2x250+8 cycles on an Illumina HiSeq 2500 instrument (with a HiSeq Rapid Flow Cell v2)