RNA-seq of Ambrosia artemisiifolia female flower
2.0 ?g of total RNA from 1F and 2F samples was used as starting material for cDNA synthesis and library construction. Enrichment of mRNA, cDNA synthesis and library preparation for Illumina NextSeq paired-end sequencing were carried out using TruSeq™ RNA sample preparation kit (Low-Throughput protocol) according to manufacturer's instructions.