GSM4674751: E14_EB_D4_MTF2null; Mus musculus; RNA-Seq
Standard RNA isolation protocol Single-cell libraries were prepared according to the sort-seq/celseq2 method Hashimony et al.(https://doi.org/10.1186/s13059-016-0938-8). Bulk-RNA libraries were prepared according to the Kapa Hyper-prep Kit (Kapa Biosystems) using NEXTflex adapters (Bio Scientific) and amplified with ten cycles of PCR. Libraries were size-selected to obtain 300bp fragments using Kapa Pure Beads and sequenced on an Illumina NextSeq machine to obtain 75bp reads
GEO Accession
GSM4674751