RNA-seq of nestling white-throated sparrow whole blood
The stranded RNAseq libraries were prepared with Illumina's 'TruSeq HT Stranded RNA Sample Prep kit'.  The libraries were pooled into four pools equimolar concentration and each pool was quantitated by qPCR and sequenced on one lane for 101 cycles on a HiSeq2500 using a TruSeq SBS sequencing kit version 4. Fastq files were generated and demultiplexed with with the bcl2fastq v1.8.4 Conversion Software (Illumina).