BGISEQ-500 sequencing
UHRR is a reference RNA sample, which has qpcr database. So the sample can be used to estimate the accuracy of high-throughput sequencing platform for RNA quantification
The RNA was fragmented after mRNA enrichment, Then the RNA fragments were reverse transcript to double-strand cDNA (dscDNA) by N6 random primer. The dscDNA were performed end repair/A-tailing, adapter ligation and PCR amplification. Finally, the PCR product were separated to single strand, followed by cyclizing to a circle library.