WGS of Duddingtonia flagrans
D. flagrans was grown on CM-agar for 7 days. Spores were  collected in 100 ml CM liquid medium and incubated at 28C for 24 h at 180 rpm. Protoplasts were generated as described above and genomic DNA was extracted using Genomic DNA Buffer Set (Qiagen) and Genomic-tip 20/G (Qiagen) according to the manufacturers instruction.