NextSeq 500 sequencing; Rat infection with Trypanosoma rhodesiense clinical isolates
Rat infection with Trypanosoma rhodesiense clinical isolates
Blood collected from infected or uninfected (control) rats into Paxgene tubes Rats were infected IP with Trypanosoma brucei rhodesiense Parasitaemias were counted in diluted blood using a haemocytometer just before blood collection. Standard Paxgene protocol on whole blood Ribo-Globin then by Illumina TruSeq Total Stranded RNA preparation kit (Illumina, RS-122-2301)
Experimental Factor: infect
Trypanosoma brucei rhodesiense
Experimental Factor: cell line
T. rhodesiense isolated from patient 42
Experimental Factor: time
12
Experimental Factor: trypanosomes measurement
78000000