16S amplicon sequencing of Knapholm non-amended BioGAC rector rep2
Total DNA was extracted using a PowerLyzer UltraClean Microbial DNA Isolation kit (Mo Bio Laboratories, Inc.). The V3-V4 region was PCR amplified with 341F (CCTAYGGGRBGCASCAG) and 806R (GGACTACNNGGGTATCTAAT). Illumina indices were added using a further PCR reaction with primers containing appropriately indexed Illumina adapters with overlaps to the V3-V4 primers.