GSM4337178: CFPAC1.HNF1B-WT #01; Homo sapiens; RNA-Seq
10^6 cells are lysed and processed according to SMART-Seq2 protocol. Briefly, 5 ng of poly-A containing mRNA molecules are copied into first strand cDNA by reverse transcription and template-switching using oligo(dt) primers and LNA-containing template-switching oligo (TSO). cDNA is pre-amplified using limited number of cycles, purified and tagmented with Tn5 transposase. cDNA fragments generated after tagmentation are gap-repaired, enriched by PCR and purified to create the final cDNA library for Illumina sequencing.
GEO Accession
GSM4337178