GSM1707598: Primed_RNAseq_technicalReplicate2; Homo sapiens; RNA-Seq
6 million naive or primed hESCs were used for each RNA extraction. Total RNA was purified using the mirVana™ miRNA Isolation Kit (Life Technologies, AM1560) following the manufacturer’s instructions. 1 μg of total RNA was used for the RNA-seq library construction. A technical replicate was performed for both naïve and primed hESCs. Polyadenylated RNA-seq libraries were prepared using the TruSeq Stranded mRNA Library Prep Kit (Illumina, RS-122-2101). The RNA-seq libraries were sequenced on the Illumina HiSeq 2000.
GEO Accession
GSM1707598