Pseudomonas aeruginosa. Population 7. Challenged with tigecycline. Evolved
Genomic DNA was extracted at the end of the evolution assays from all 12 populations using the Gnome DNA kit (MP Biomedicals). Whole-genome sequencing was performed by Sistemas Genmicos S.L. Libraries were obtained without amplification following Illumina protocols and recommendations. The quality of the extracted material was analyzed via a 4200 Tapestation High Sensitivity assay, and the DNA concentration determined by real-time PCR using a LightCycler 480 device (Roche).