Mouse Isolate of Klebsiella pneumoniae  1808200021 blood
Bacterial pellets obtained from overnight growth in LB medium were used for genomic DNA purification by the Roche High Pure PCR Product Purification kit.  Barcoded libraries were constructed using the QIAseq FX DNA Library Kit and sequenced by Illumina MiSeq 2x300 reads.  Raw sequence reads were decontaminated of adapter sequences and quality trimmed to a Phred quality score (Q)10 using BBDuk from the BBMap package version 38.34 for de novo assembly into contigs with SPAdes version 3.10.0 and genome annotation by RAST, both hosted by PATRIC (Pathosystems Resource Integration Center)