GSM2064470: Control RNAi batch 6; Homo sapiens; RNA-Seq
Total RNA from HeLaK cells subjected to PWWP2A siRNAs and control siRNAs was isolated utilizing the RNeasy mini kit (Qiagen) according to manufacturer’s instructions. The quality of the RNA (amount and integrity) was assessed using the BioAnalyzer and a RNA 6000 pico kit (Agilent). mRNA isolation, fragmentation, first and second strand cDNA synthesis and end repair were carried out using the NEBNext Ultra RNA library prep kit for Illumina (NEB) following the manufacturer’s instructions cDNA sequencing libraries were established with the MicroPlex Library Preparation Kit (Diagenode) as described above
GEO Accession
GSM2064470