RNA-seq of human iPSC differentiation towards keratinocytes: Sample ctr1_PSC_d4_RNAseq_ReadCountperGene_2
The NuGEN Ovation RNA-Seq (version 2, NuGEN) protocol was carried out on 100 ng of initial RNA. Ribosomal RNA (rRNA) was removed using targeted depletion with the NuGEN Ovation RNA-Seq platform (NuGEN) using the InDA-C Technology.RNA-seq libraries were amplified using 10 cycles. The purified PCR products were then quantified using an Agilent bioanalyzer (DNA-1000 kit). We selected 300bp fragments using E-Gel™ SizeSelect™ II Agarose Gels, 2% (E-Gel, GIBCO). 
alignment_software
hisat2