ANTICANCER EFFECT
Amplicons were extracted from 2% agarose gels and purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences Union City CA  US) according to the manufacturer instructions and quantified using QuantiFluor? -ST-Purified amplicons were pooled in equimolar and paired-end sequenced on an Illumina MiSeq platform according to the standard protocols