Illumina HiSeq 2000 sequencing; Transcriptome profiling of NGLY1 deficient K562 cells
Transcriptome profiling of NGLY1 deficient K562 cells
Cells were grown in DMEM (Gibco, 41965-039) with 10% FCS and, if necessary, supplemented with Puromycin (4 microg/microl) and Blasticidin (3.5 microg/microl) at a confluence between 20% and 80%. Full protocol details can be found in Mueller and Jakob et al, 2020.     total RNA was extracted using trizol acording to manufacturer's specifications. Full protocol details can be found in Mueller and Jakob et al, 2020.  A cDNA library was created using the Illumina TruSeq Kit according to manuacturer's specifications.  Full protocol details can be found in Mueller and Jakob et al, 2020.
Experimental Factor: Genotype
wild type