16S-Miseq sequencing from pooled guts of A.echinatior lab colony Ae153 reared on fungus garden -replicate 35C2
"16S rRNA gene libraries were constructed using different indexed primers to amplify the V34, V4, and V45 regions from genomic DNA. The purified amplicons were pooled in equimolar concentrations, the final concentration of the library was determined and was sent for sequencing."