GSM3613338: Hi-Myc, GDC002 treatment Rep 2; Mus musculus; RNA-Seq
RNAs were extracted from dorsolateral prostates (DLPs) of Genetically Engineered Mouse Models (GEMMs, 5 months of age) by using the the pureLink RNA Kit (Invitrogen). Ribosomal RNA removal was carried out using the RiboMinus Human/Mouse Transcriptome Isolation Kit (Invitrogen) according to the manufacturer's protocol. RNAs derived from GEMM DLPs were subjected to next-generation sequencing (NGS) to generate deep coverage RNA-Seq data. RNA-seq libraries were prepared using Illumina TruSeq RNA Sample Prep Kit V2 and subjected to deep sequencing with Illumina HiSeq 4000 at the Molecular Biology Core Facilities at Dana Farber Cancer Institute. To achieve comprehensive coverage for each sample, we generated ~32-39 million single end reads.
GEO Accession
GSM3613338