GSM3930359: H226 CONTROL 2; Homo sapiens; RNA-Seq
Total RNA isolated from frozen cell pellets was extracted using Invitrogen PureLink RNA Mini Kit Spin Column 250 (cat #12-183-025). RNA was quantified and assesed for integrity using the  Agilent RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA). Libraries were constructed using Illumina TruSeq stranded RNA kit with Ribo-Zero Globin kit following the manufacturer's instructions (Illumina, San Diego CA)