GSM1943653: Wor4-GFP Opaque Cell 2 (1304B); Candida albicans; ChIP-Seq
Cells were lysed with glass bead vortexing for 2 hours, sonicated for 2 15-minute cycles (30s on, 60s off), and immunoprecipitated at 4˚C overnight (5µL of Living Colors Full-Length GFP Polyclonal Antibody (Clontech 632592), Protein A-Sepharose 4B Fast Flow (Sigma P9424)). Libraries were prepared using the NEBNext ChIP-Seq library prep kit for Illumina (New England Biolabs)
GEO Accession
GSM1943653