Transcriptomics throughout sex change in Amphiprion bicinctus
NEBNext Ultra Library Prep Kit for Illumina according to manufacturer’s instructions, using 12 cycles of PCR for enrichment. 64 samples in total (32 brain + 32 gonadal tissues), two pools of 20 samples plus two pools of 12 samples were ran in four lanes.  Two lanes of a MiSeq sequencer ( with 4 samples per pool) were used for resequencing of samples displaying a significantly lower number of reads in the previous run.