Folsomia candida, suppression treatment – replicate 3
Springtails were sampled from a population kept in standardized culturing conditions for several years. Four replicate samples were collected, each consisting of ten animals, and used for DNA isolation and targeted PCR. Each sample was amplified using two different approaches, “Suppression” and “Control”. The Suppression treatment consisted of a nested PCR approach to suppress amplification of Wolbachia DNA: first, a PCR was performed with primers 8f and 1525R, and the resulting PCR products were amplified with primers 357F and 518R, with appropriate barcodes and Illumina linkers.