GSM2065260: Stage-16_rep3; Ambystoma mexicanum; RNA-Seq
We then extracted total RNA from homogenized samples using the RNeasy Mini Kit (Qiagen). Samples for Paired-end sequencing  were prepared via the Illumina TruSeq RNA protocol (PolyA selection). Samples for single-end sequencing were prepared via the Illumina ScriptSeq protocol (Ribo-zero).  Paired-end samples are pooled (six per lane) and sequenced 2 x 101bp paired end reads on the Illumina HiSeq 2000 with TruSeq v3 chemistry and Single-end samples are pooled and sequenced at the length of 65bp.
GEO Accession
GSM2065260