GSM3015416: lean_2; Homo sapiens; RNA-Seq
Total RNA was extracted from samples of cohort 1 using the RNeasy Lipid Tissue Mini Kit (74804, Qiagen, Hilden, Germany) as per manufacturer's instructions. RNA concentration and purity was measured using a using Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific, Lafayette, USA). RNA libraries for sequencing were prepared using TruSeq RNA kits (Illumina, CA, USA) according to the manufacturer's instructions with the following changes. The protocols were automated using an MBS 1200 pipetting station (Nordiag AB, Sweden). All purification steps and gel-cuts were replaced by the magnetic bead clean-up methods as previously described (Borgstrom et al., 2011). The samples were sequenced on an Illumina HiSeq 2000 as paired-end reads to 100 bp.
GEO Accession
GSM3015416