H3K9me3 ChIP-Seq analysis of cortex neural stem and progenitor cells from HuFNSC01 (A03271)
H3K9me3 ChIP-Seq analysis of cortex neural stem and progenitor cells from HuFNSC01 (A03271) using Illumina HiSeq 2000
Refer to document 'Chromatin Immunoprecipitation (ChIP)' from BC at the Roadmap Epigenomics Project site, Experimental Protocols page (URL: http://www.roadmapepigenomics.org/protocols/type/experimental/)
EXPERIMENT_TYPE
Histone H3K9me3
EXTRACTION_PROTOCOL
Refer to document 'Chromatin Immunoprecipitation (ChIP)' from BC at the Roadmap Epigenomics Project site, Experimental Protocols page (URL: http://www.roadmapepigenomics.org/protocols/type/experimental/)
EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR
Fisher 550 Sonic Dismembrator
EXTRACTION_PROTOCOL_SONICATION_CYCLES
10 min
CHIP_PROTOCOL
Refer to document 'Chromatin Immunoprecipitation (ChIP)' from BC at the Roadmap Epigenomics Project site, Experimental Protocols page (URL: http://www.roadmapepigenomics.org/protocols/type/experimental/)
CHIP_PROTOCOL_CHROMATIN_AMOUNT
5 ug
SIZE_FRACTION
74-314 bp
CHIP_PROTOCOL_BEAD_TYPE
Sepharose A/G Bead Mix
CHIP_PROTOCOL_BEAD_AMOUNT
20 ul
CHIP_PROTOCOL_ANTIBODY_AMOUNT
5 ug
CHIP_ANTIBODY
H3K9me3
CHIP_ANTIBODY_PROVIDER
Diagenode
CHIP_ANTIBODY_CATALOG
pAb-056-050
CHIP_ANTIBODY_LOT
A93-0042