Rickettsia felis str. LSU-Lb infecting Liposcelis bostrychophila
Total RNA from Rickettsia felis infecting Ctenocephalides felis was isolated using Trizol reagent.  RNA was treated with Ribo-Zero Magnetic Gold Kit, Ribo-Zero Magnetic Kit-Gram Negative Bacteria, and the NEBNext Poly(A) mRNA Magnetic Isolation Module.  Sequencing Library was constructed using the Ion Total RNAseq Kit v2.  Sequencing was performed on a 316 size chip on the Ion Torrent Personal Genome Machine. Sequenced reads were assessed for quality and aligned to the URRXWCal2 genome using the Ion Torrent pipeline v2.2.1.