RNAseq of Nicotiana benthamiana: mock infiltrated leaf, 10 dpi
The mRNA fraction was selected from the total RNA provided before conversion to cDNA using oligo-dT beads. Second strand cDNA synthesis incorporated dUTP. The cDNA was end-repaired, A-tailed and adapter-ligated. Prior to amplification, samples underwent uridine digestion. The prepared libraries were size selected, multiplexed and QC’ed before paired end sequencing over three lanes of a flow cell.