Bulk segregant analysis of Saccharomyces cerevisiae progeny from cross 322134S x YPS128, initial pool
Genomic DNA was extracted from pools of yeast cells using a Qiagen Pure Yeast Gene kit. Sequencing libraries were prepared by fragmentation using a Covaris and NEBNext reagents using TruSeq adapters and TruSeq SBS Kit v3 for sequencing. This sample represents the pooled progeny before passaging.