GSM2671196: Dextrose exponential phase, biological replicate 2; Mycobacterium tuberculosis H37Rv; RNA-Seq
Cell pellets were resuspended in the lysis buffer (6 M guanidinium chloride, 0.1% Tween 80, 1 mM 2-mercaptoethanol, 10 mM EDTA) and were lysed mechanically in a FastPrep cell disrupting equipment with 150 μm - to 200 μm glass beads by performing three lysis cycles of 45 s, at 4.0 m/s followed by two lysis cycles of 15 s at 6.5 m/s. Nucleic acids were extracted with phenol-chloroform-isoamyl alcohol (25:24:1) and RNA was precipitated with 0.4 volume of absolute ethanol. Total RNA was purified three times with Trizol reagent. RNA was dissolved in DEPC-treated water and stored at -70°C. Library preparation was performed using the 'NEBNextUltra Directional RNA Library Prep Kit for Illumina' (New England Biolabs), according to manufacturer's protocol.
GEO Accession
GSM2671196