Zymoseptoria brevis YMS RNA sequencing
Total RNA was extracted from fungal cells grown in YMS (4 g yeast extract, 4 g malt extract, 4 g sucrose, 20 g bacto agar, 1 l H2O) agar in a shaking incubator at 200rpm at 18°C using the TRIZOL reagent (Invitrogen, Darmstadt, Germany) following the manufacturers protocol. Illumina RNA-seq libraries for two axenic culture replicates per species were prepared from an input of 10 µg total purified polyA RNA. Libraries were quantified by fluorometry, immobilized, and processed onto a flow cell with a cBot (Illumina) followed by sequencing-by-synthesis with TruSeq v3 chemistry on a HiSeq2000 at the Max Planck Genome Center (Cologne, Germany)