GSM2064615: 16_LS; Homo sapiens; RNA-Seq
TissueLyser LT homogenizer (Qiagen, USA) was used to homogenize biopsy specimens. Total RNA was extracted with ExtractRNA reagent (Evrogen, Russia) according to the manufacturer’s protocol. Isolated RNA was dissolved in RNase free water, rRNA was depleted using RiboMinus™ Eukaryote Kit for RNA-Seq (Life Technologies, USA), and the samples were stored at -80C°. The quality of total RNA was evaluated with RNA 6000 Pico Chip Kit and Agilent 2100 Bioanalyzer (Agilent Technologies Inc., USA) and with Quant-iT™ RNA Assay Kit and Qubit Fluorometer (Life Technologies, USA). The average RNA integrity number (RIN) of samples was ≥7. Library preparation and sequencing were performed using SOLiD 4 System platform and sequencing chemistry according to the manufacturer’s instructions (Life Technologies, USA).
GEO Accession
GSM2064615