GSM4066600: Line 2, Flight rep2 [FB5]; Homo sapiens; RNA-Seq
RNALater (Thermo Fisher) was used to preserve flight samples designated for downstream RNA-sequencing analysis. Samples preserved in RNALater were stored at 4°C until return to our laboratory, when RNA was isolated from preserved wells in the BioCells using RNeasy kit (Qiagen) following the manufacturer's protocol. DNase treatment was performed using RNase-free DNase kit (Qiagen). All RNA samples had RNA integrity number ≥ 8.9 and concentration ≥ 48 ng/µL (Agilent 2100 Bioanalyzer). Library prep and sequencing were performed by BGI with 30-40 million paired-end 100bp reads per sample on the Illumina HiSeq platform.
GEO Accession
GSM4066600