GSM2224573: Sox2 KO stomach 1 [RNA-seq]; Mus musculus; RNA-Seq
Stomachs were laid out flat, cleaned, and pylorus was carefully excised. The pylorus was then placed in 5 mL of Corning Cell Recovery solution for 1 hour at 4 degrees. After this incubation, the muscle layer was gently separated from the epithelium/mesenchyme using fine tweezers. RNA was extracted from the epithelium/mesenchyme layer using the RNeasy kit from Qiagen according to their protocol. 1 ug of total RNA was used for the construction of the sequencing libraries RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM2224573