Glycine max raw sequence reads
In total, 500 mg endosperm tissues from fresh 35-DAF seeds were ground with liquid nitrogen, and total RNA was extracted with TRIzol reagent (GibcoBRL, USA) as described (Shih et al., 2004), and then was enriched by Plant RNeasy mini kit (Qiagen, USA). The library prepared using mRNA-Seq sample prep kit (Illumina Inc. USA) according to the manufacturer’s protocol. All samples were purified and multiplexed using eight of the three-nucleotide barcoded adapters. The library was sequenced using either Illumina’s GAII sequencing system or the HiSeq 2000 (Illumina Inc., USA).