GSM4183164: WT3; Mus musculus; RNA-Seq
Spleens were preserved in RLT Buffer at -80°C until analysis. The samples were processed and analysed by GenXPro GmbH (Goethe University, Frankfurt, Germany). RNA of the Treg was extracted using Zymo IC columns (Zymo), as described by the manufacturer. DNAse digestion was performed using DNAseI (NEB) and the RNA was purified using the “Zymo RNA Clean & Concentrator Kit” (Zymo). SmallRNA libraries were prepared using the “TrueQuant SmallRNA-Seq” kit (GenXPro) according to the manufacturer's instructions.
GEO Accession
GSM4183164