GSM4560367: AA rep2; Mus musculus; RNA-Seq
Mice were perfused with PBS, kidneys removed and stored in RNAlater. Mouse kidneys were sliced and tubule microdissection was performed in ice-cold dissection solution (135 mM NaCl, 1 mM Na2HPO4, 1.2 mM Na2SO4, 1.2 mM MgSO4, 5 mM KCl, 2 mM CaCl2, 5.5 mM glucose, 5 mM HEPES, 0.1% BSA; pH 7.4) using a Leica MZ12.5 stereomicroscope equipped with Leica MC170 HD microscope camera and ThermaZone cooling device. Five to ten S2/S3 PT tubules were pooled together from each kidney and washed with PBS in a separate petri dish. RNA was extracted using PicoPure RNA kit (Applied Biosystems). cDNA was prepared using TruSeq Stranded mRNA Sample Preparation kit (Illumina, San Diego, CA), according to the manufacturer's instructions.
GEO Accession
GSM4560367