LEQUIA06
Sequences were modified by addition of Fluidigm CS1 oligo to the 5' end of the forward and CS2 oligo to the 5' end of the reverse primer. Secondary PCR targeting the CS1 and CS2 sequences was used to add dual indexed, Illumina compatible adapters. Final PCR products were normalized using an Invitrogen SequalPrep DNA Normalization plate and recovered samples were pooled. The pooled samples were given a final clean up with AmpureXP beads.