shotgun of 295C
Shotgun sequencing was performed on the Illumina NextSeq Sequencer platform (150 bp read length, paired-end protocol). Samples were split between 2 runs with minimal batch effect (data not shown). DNA libraries were generated following the Nextera XT Illumina protocol using the Nextera XT Library Prep Kit (#FC-131- 1024, Illumina). The protocol was initiated with 0.2ng/L purified gDNA. Multiplexing was performed using the Nextera XT Index Kit (#FC-131-1096, Illumina). Libraries were sequenced using a 2x150 bp paired-end cycle with the NextSeq High Output Reagent Kit (Illumina) on a NextSeq Sequencer according to the manufacturers instructions.