GSM4066490: S64912 [lib37847_CD2Y3ANXX]; Homo sapiens; RNA-Seq
Fresh blood was collected and granulocytes were extracted from the left over pellet after Ficoll purification by lysing RBCs with RBC lysis buffer. Granulocytes were then washed 3X with DNAse- and RNAse-free PBS and then lysed in RLT/bME buffer. After a 30min incubation at 4 degrees, RNA was isolated using the Qiagen RNA preparation kit. Reverse transcription was performed followed by PCR amplification to generate full length amplified cDNA. Sequencing libraries were constructed using the NexteraXT DNA sample preparation kit (Illumina) to generate Illumina-compatible barcoded libraries. Libraries were pooled and quantified using a Qubit® Fluorometer (Life Technologies).
GEO Accession
GSM4066490