Prokaryotic community in decaying Pieca abies logs
DNA was extracted from the wood samples as described in Rajala et al. (2012). Originally one DNA sample originated from one log. These individual DNA samples were sorted according to wood density within each study site. Subsequently ten DNA samples with similar wood densities were pooled.  Prokaryotic community composition was studied through sequencing of bacterial 16S amplicons amplified with primers Eub338 and Eub518 (Fierer, et al., 2005) and archaeal 16S amplicons amplified with Arch349F and Arch806R (Takai & Horikoshi, 2000). 454-sequencing adapters and barcodes of 10 nucleotides were ligated into purified qPCR products in secondary PCR reactions.