RNA-Seq of hybrid poplar:mature DX
Total RNA was isolated using the cetyl trimethylammonium bromide (CTAB) method with slight modification (Logemann et al. 1987). In brief, fine powder of plant tissues was mixed with CTAB buffer followed by phenol: chloroform: isoamyl alchol (25: 24: 1) extraction. Isoprophanol was added to the mixture to isolate RNA. The quality of the RNA was estimated using an Agilent 2100 Bioanalyzer (Agilent, Santa Clare, CA). One microgram of total RNA from each samples was used for the RNA sequencing library construction following the Illumina TruSeq RNA library type (Illumina, San Diego, CA). Libraries were paired-end sequenced on an Illumina HiSeq2000 platform (Illumina, San Diego, CA)