Bacteria in paddy soil chronosequence: Sample S50
The total soil DNA was isolated and purified using a FastDNA spin kit (MP Biomedicals, Santa Ana, CA, USA), and then the V4-V5 region of the bacterial 16S rRNA gene was amplified using the primer pair 515F-907R. The prepared library was loaded on a MiSeq Reagent Kit V2, and sequencing of paired-end 250 bp was run on an Illumina MiSeq apparatus after prepared according to the MiSeq Reagent Kit preparation guide (Illumina, San Diego, CA, USA).