GSM1704224: K498A Input; Homo sapiens; RIP-Seq
Two confluent 10cm dishes of cells were lysed in isotonic lysis buffer (per sample). Extracts were immunoprecipated with M2 FLAG magnetic beads (Sigma).  Anti-FLAG immunoprecipitated RNA was extracted with Trizol (Invitrogen) and made into a library using the Illumina TruSeq stranded total RNA library prep kit with Ribo-Zero Gold
GEO Accession
GSM1704224