Whole genome sequencing of independently derived 5FC resistant colonies of Cryptococcus deuterogattii
Single colonies were expanded in 50 mL YPD liquid cultures with 5FC. Cultures were concentrated by centrifugation and lyophilized. DNA was then isolated using a CTAB DNA prep, and the DNA was treated with RNAase. DNA was re-extracted using phenol-chloroform. Paired end libraries were then prepared using a Kapa hyper prep and sequenced by the Duke University Sequencing Core using the NovaSeq platform.