amplicon sequencing of Fungal DNA: Sarracenia purpurea carnivorous pitcher water
Water was collected aseptically from Sarracenia purpurea carnivorous pitchers. DNA was extracted using a chloroform-phenol protocol, and fungal DNA was amplified using the primers ITS1F and ITS4, with ITS1F concatenated with the 454 B primer and ITS4 concatenated with the 454 A primer and a multiplex tag. Samples were purified, combined into a single library, and run on a 454 Titanium machine.