RNA-Seq of homo sapiens: breast cancer SUM159PT cells
Total RNA was isolated from shControl and shADAM12 SUM159PT cells ± doxycycline (n=3 for each condition) using the Qiagen RNeasy purification kit and on-column DNase I digestion according to the manufacturerÕs protocols. An NGS library was generated for each RNA sample using the Illumina TruSeq Library Prep kit.