genome wide association of aggression in gentle Africanized honey bee
Libraries of 250bp insert were constructed and sequenced for each sample using the BGI-Seq 500 platform, resulting in approximately 5Gbp data for each sample.  The protocol repaired the DNA fragment ends by T4 DNA Polymerase and T4 Polynucleotide Kinase, ligated adapters via T4 DNA ligase, and filled-in of adapters with Bst Warmstart Polymerase. Following these steps libraries were purified via the QiaQuick purification kit (QIAGEN, Shanghai, China ) in silica columns. Purified DNA libraries were amplificated through AmpliTaq Gold polymerase (Applied Biosystems, Foster City, CA) quantified by Qubit fluorometer.