J25 small RNA library, replicate 2
2ug of RNA were  incubated with 5 RNA polyphosphatase (Epicentre) at a final concentration of  1U/l at 37C for 1h in a total volume of 20ul, to convert 5PPP 22G-RNAs into  clonable 5P 22G-RNAs. RNA was phenol-chloroform extracted and precipitated  overnight with 1l glycogen, 1/10 volume 3M AcNA and 3 volumes 100% ethanol.  Small RNA libraries were generated using the Illumina TruSeq Small RNA  library preparation kit as per manufacturers instructions, and gel-purified  to size select 21-23nt inserts