GSM1622422: Input_TN; Mus musculus; ChIP-Seq
E 16.5 embryonic brains were isolated and downstream processing was done in cold HBSS. Cortical region of brains were micro dissected and processed for single cell dissociation using Neural Tissue Dissociation Kit (P) (130-092-628) following the manufacturer's instructions. GFP positive live cells were FACS sorted directly into RLT lysis buffer and RNA was isolated using RNeasy Micro Kit (Qiagen, 74004) following the manufacturer's instructions. For RNA-seq samples, PolyA library type was used.
GEO Accession
GSM1622422