Antibiotic resistance genes and potential pathogens in streambed biofilms impacted by raw and treated wastewater: Does the pollution source matter?
High-throughput multiplexed 16S rRNA gene sequencing with the Illumina MiSeq System (2×250 PE) was carried out using primer pair 515f/806r targeting the V4 region of the 16S rRNA gene complemented with Illumina adapters and sample-specific barcodes at the genomics core facilities and methods of the Research Technology Support Facility Michigan State University, USA.