GSM1522206: epiRIL_260; Arabidopsis thaliana; Bisulfite-Seq
Qiagen DNeasy Fragment genome DNA to 100-300bp by Sonication, DNA-end repair, 3’-dA overhang and ligation of methylated sequencing adaptors, Bisulfite treatment by ZYMO EZ DNA Methylation-Gold kit, Desalting, size selection, PCR amplification and size selection again.