GSM1897047: G3-1: 1.0% green tea powder group Repeat1; chicken gut metagenome; OTHER
The ileum were thawed and the contents were gently scraped from the ileal walls. Genomic DNA was extracted from each of the 12 samples using the Bacterial Genomic DNA Extraction Kit (Tiangen Biotech, Co., LTD, China). The bacterial genomic DNA was amplified with the 343F (5‘-TACGGRAGGCAGCAG-3‘) and 798R (5‘-AGGGTATCTAATCCT-3‘) primers, which are specific for the V3-V4 hyper-variable regions of the 16S rRNA gene. Each primer incorporated FLX Titanium adapters and a sample barcode at the 5’ end of the forward primer to allow all samples to be mixed in the 1/4 454 FLX+ sequencing run