GSM4563206: H3K27ac_1153_input_rep1; Homo sapiens; ChIP-Seq
The tissue homogenate was filtered with a 70 µm cell strainer and fixed in 1% formaldehyde at room temperature for 10 min. The tissue pellet was collected through centrifugation, washed twice with cold PBS and then incubated with lysis buffer on ice for 20 min. The tissue lysate was sonicated, and DNA was sheared to an average size of 100-300 bp. Approximately 10% of the total tissue lysate was collected as input, while the other portion was first precleared with uncoupled Protein-A Dynabeads (Novex, 10002D) and incubated with H3K27ac-coupled Protein-A Dynabeads for 6 hours in a cold room. Then, the Dynabeads were collected using a magnet track, and chromatin was eluted. The DNA was then processed for NGS library construction and sequenced.