GSM4223755: RplusGminus3 miRNA-seq; Mus musculus; miRNA-Seq
Brain of E14.5 embryos were dissected to remove meningies, ganglionic eminences and olofactory bulb. The isolated lateral cortex was then dissociated using the Neural Tissue Dissociation Kit with papain (Miltenyi Biotech, cat. no. 130-092-628) according to the manufacturer's protocol and cell suspension was then sorted with a BD FACS Aria III using the reporter endogenous fluoresence. Sorted cells were kept on ice and total RNA was then extracted using the Quick RNA Mini Prep (Zymo Research, cat. no. R1054) Libraries were prepared using the NEBNext Small RNA Library Prep Kit for Illumina (NEB, cat. no. E7330L)