GSM2413350: Agrp-IRES-Cre::L10-GFP+ neuron #10; Mus musculus; RNA-Seq
fluorescently-labeled and -unlabeled cells were manually isolated from a cell suspension made from triturating freshly-dissected, papain-digested PVH and ARC Single cells were frozen at -80degC and then processed into RNA-seq libraries using a published protocol (Tang, F. et al. RNA-Seq analysis to capture the transcriptome landscape of a single cell. Nat. Protoc. 5, 516–535 (2010)) and NuGen's Rapid Library DR kit according to manufacturer's instructions