GSM3391182: S_R64_C51; Mus musculus; RNA-Seq
Cells were dispensed into printed chip (430-000233, Wafergene) using MultiSample NanoDispenser (MSND).  The chip loaded with cells was imaged using an Olympus BX43 fluorescent microscope to locate wells with single cells using CellSelect software (Takara Bio USA). To break cells for downstream analysis, the chip was frozen at -80°C for more than one hour before thawed. We employed the iCell8 single cell system from Wafergen BioSystems for single cell capture to achieve an intermediate in-depth expression profiling of single prostate stromal cells.  In-chip RT-PCR amplification and single cell RNA-seq library construction were performed following the iCELL8 single cell polyA transcriptome amplification hands-on workflow checklist and preparation guide.