Griffinia hyacinthina MiSeq Feb2014
Sequencing libraries were constructed using half reactions of the NEBNext® DNA Library Prep Master Mix Set for Illumina® kit (New England Biolabs, Ipswich, Massachusetts) and following the manufacturer’s protocol. Sequence capture was performed with MYbaits kit following the manufacturer’s protocol (MYcroarray) and the first additional step suggested by Mandel et al. (2014). Sequencing of 20-plexed target-enriched library was conducted at the Biotechnology Center, University of Wisconsin-Madison (UW-Madison).