GSM4073091: Tg-3 EE; Mus musculus; RNA-Seq
The upper colon tissues section (2-3 cm piece) was taken from the WT and TG mice (SE and EE conditions). Colon was then prepared rapidly on ice, feces material was flushed with PBS and then colon tissue was rinsed again with PBS and rinsed tissue was briefly dried on tissue towel to remove excess PBS. The rinsed colon tissue was then collected in cryotubes and snap frozen by liquid N2 and stored  at -80 0C until use. Sequencing libraries have been prepared with Lexogen QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina following the manufacturer's handbook.