NextSeq 500 sequencing; Jinfukang enhances the pro-apoptotic activity of cisplatin via activation of AIFM2 in human lung cancer cells
Jinfukang enhances the pro-apoptotic activity of cisplatin via activation of AIFM2 in human lung cancer cells
Cells were cultured in RPMI 1640 Medium (Gibco, USA). Cultures were supplemented with 10% fetal bovine serum (FBS) (Gibco, USA) and 100 ug/ml penicillin/streptomycin (Life Technologies, Inc. USA). A549 cells were exposed to JFK (30 μg/ml) and DDP (6 μg/ml) for 48 h, alone or together. Total RNA of each sample was extracted by Trizol (Life Technologies, Inc. USA) according to manufacturer’s protocol. mRNA was isolated via Oligotex mRNA Mini Kit (Qiagen, Germany), and then treated with DNase for removing residual genomic DNA. 100 nanogram (ng) mRNA of each sample was fragmented, end-repaired, and ligated adapters using NEBNext Ultra Directional RNA Library Prep Kit (NEB, USA). Each sample was amplified 12 cycles in thermal cycler.