GSM2491731: HTS-2 [miRNA]; Oreochromis niloticus; miRNA-Seq
The samples were used for further processing. About 1 ug total RNA of each sample was employed for constructing small RNA library based on protocol of TruSeq Small RNA Sample Prep Kits (Illumina, San Diego, USA). Subsequently, we used the Illumina HiSeq 2500 and single-end sequencing (50 bp) to analyze small RNA library according to standard methods at the LC-BIO (Hangzhou, China). Following the manufacturer's procedure, total RNA of liver samples was extracted by using of Trizol reagent (Invitrogen, CA, USA).