Untreated Langerhans Cell-Deficient-2
A 148 bp fragment of Hras exon 2 was pcr amplified using barcoded primers (143 bp Hras   5 bp barcode).  PCR products were purified using Qiagen QIAquick PCR Purification Kit. Parcoded PCR products were pooled (100 ng each) and submitted to the Yale Center for Genomic Analysis (YCGS).  The pooled amplicons were conbined at a 1:1 ratio with an Illumina PhiX library prior to sequencing.