GSM1524922: MEF_GRO-Seq; Mus musculus; OTHER
GRO-Seq: BrU labelled nascent transcripts were extracted with Tripure reagent and affinity purified with anti-BrU antibody conjugated agarose beads. GRO-Seq: BrU labelled nascent RNA transcripts were fragmented with NaOH, and RNA was decapped with Tobacco Acid Pyrophosphatase (TAP) and end-repaired with T4 polynucleotide kinase. Illumina Adapters were ligated to the RNA with T4 RNA ligase and cDNA was generated with Reverse Transcriptase. The libraries were PCR amplified with Illumina primers and subjected to Illumina Hiseq2000 or HiSeq2500.