16S of Pyropia interalgal microorganisms:  enviromental seawater of Pyropia wich rehabilitative from yellow spot disease  after treatment
Total genome DNA of the samples was extracted by CTAB method (Doyle 1987). The V3V4 regions of 16S rRNA gene were amplified with the primer pair: 341F (5-CCTAYGGGRBGCASCAG-3) and 806R (5-GGACTACNNGGGTATCTAAT-3). PCR reactions were carried out in 30L reactions with 15L of Phusion High-Fidelity PCR Master Mix (New England Biolabs). The samples with bright main strip between 400-450bp were chosen for sequencing.