GSM4498839: CS2; Mus musculus; RNA-Seq
Total RNA from mouse RPE/choroid using triplicate biological replicates for air or smoking were isolated using RNeasy Mini Kit (Qiagen, Valencia, CA), with on-column DNA digestion by RNase-free DNase, following the manufacturer's instructions. RNA integrity was verified using Agilent 4200 TapeStation (Agilent, Santa Clara, CA). Stranded RNA-seq library construction was carried out using 100 ng of RNA with the TruSeq Stranded mRNA Sample Preparation Kit (Illumina, San Diego, CA). Libraries were paired-end sequenced to 126 bases using a HiSeq 2500 Sequencing System (Illumina, San Diego, CA).