GSM2071055: DN111_IL12_D505_D702; Homo sapiens; RNA-Seq
Total RNA was purified using miRNAeasy Mini kit (Qiagen). Standard quality control steps were included to determine total RNA quality using Agilent Bioanalyzer (RNA integrity number (RIN) > 8.5; Agilent RNA 6000 Nano Kit, USA) and quantity using ananoliter spectrophotometer (Nanodrop, Thermofisher, USA). For every sample, 500ng of purified total RNA was prepared into mRNA libraries, according to manufacturer’s instructions, using the Truseq Stranded mRNA Library Prep Kit (Illumina, RS-122-2103).