GSM3389516: NfkB2-/- Input; Mus musculus; ChIP-Seq
Cell nuclei were isolated and lysed. The crosslinked chromatin was subjected to fragmentation by sonication (Branson Sonicator). Chromatin immunoprecipitation (ChIP) was performed using anti-RelA antibody (Santa Cruz, sc-372) or anti-RelB antibody (sc-226) bound to 50μl Protein A Dynabeads (Invitrogen). ChIP seq library were prepared using Nugen Ovation Ultra-low library preparation kit version 2 according to the manufacturer's protocol (Nugen, San Carlo, CA).  The libraries were sequenced on Illumina NextSeq 500 for single end reads