Hi-C sequencing of opium poppy var. PS7
Chromatin conformation capture data of PS7 was generated using a Phase Genomics Proximo Hi-C Plant Kit. Following the manufacturer's instructions, intact leaf cells were crosslinked using a formaldehyde solution, digested using the Sau3AI restriction enzyme, and proximity ligated with biotinylated nucleotides to create chimeric molecules composed of fragments from different regions of the genome that were physically proximal in vivo, but not necessarily genomically proximal. Continuing with the manufacturer's protocol, molecules were pulled down with streptavidin beads and processed into an Illumina-compatible sequencing library.