GSM2070660: Het (Q140) 6 months Female Cerebellum mRNA rep3 398812_63521_1_L4.D709; Mus musculus; RNA-Seq
Tissue specimens were removed, flash frozen on dry ice, and total RNA was harvested using the Qiagen miRNeasy kit. RNA quality and integrity were monitored via Agilent Bioanalyzer. A minimum of 3.75 ug of total RNA were used to collect enriched RNA subfractions for library construction. RNA libraries were prepared for sequencing using standard Illumina protocols.