Deep Sequencing of Off Target Site 2 in treated blastocyst 3
Sites of partial homology to the PNA binding site were identified. Blastocysts then underwent whole genome amplification. Amplicons were then generated and nucleotide changes from the reference genome were analyzed. The primers used for off target site 2 were (forward first): (5-NNN NNN CCCAATCCTGAATCCTGGCT-3 and 5-NNN NNN CATACTGATGTCTGTGGCTTGA-3); Each N is a random base.