GSM4565369: CtNecOt2; Mus musculus; RNA-Seq
At day 3 of osteoclast culture, RNA was harvested using peqGOLD TRIfast (Peqlab) according to the standard protocol. NEBNext® UltraTM RNALibrary Prep Kit for Illumina® (NEB, USA) was used with 1 µg of total RNA for the construction of sequencing libraries. Clustering of the index-coded samples was completed on a cBot Cluster Generation System using PE Cluster Kit cBot-HS (Illumina), according to the manufacturer's instructions. The library preparations were sequenced on an Illumina HiSeq platform and paired-end reads were generated.