GSM3931666: HepG2 IgG for EZH2 2nd [CLIP-seq]; Homo sapiens; OTHER
For immunoprecipitation, the supernatant was incubated overnight at 4 °C with 15ug antibody or control IgG antibody. Carry the recovered RNA through a standard cDNA library preparation protocol originally described for cloning of small regulatory RNAs. For high-throughput sequencing, the libraries were prepared following the manufacturer's instructions and applied to Illumina NextSeq 500 system for 100 nt pair-end sequencing by ABlife, Inc (Wuhan, China).