GSM4498955: Os9: Control R1; Oryza sativa Indica Group; RNA-Seq
Collected leaf tissues were immediately frozen in liquid nitrogen. Total RNA was extracted using Trizol (Invitrogen Inc., Carlsbad, CA, USA) and treated with DNase I (Invitrogen Inc., Carlsbad, CA, USA). Illumina TruSeq RNA Sample Preparation® V2 (IlluminaTM) kit was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols