GSM4072532: RNAseq of Bordetella pertussis BPSM DBvgA 1; Bordetella pertussis; RNA-Seq
Bacteria were pelleted, and total RNA was extracted using TriReagent (Invitrogen) following the manufacturer's instructions. Genomic DNA was removed by DNase I treatment (Sigma Aldrich). For each RNAseq sample, DNA-depleted total RNA was treated with the Ribo-Zero rRNA Removal Kit (Illumina) following the manufacturer's recommendations. The rRNA-depleted RNA was then used to build the Illumina library using the TruSeq RNA Library Preparation Kit, followed by sequencing on an Illumina NextSeq 500 benchtop sequencer on SR150 high output run mode.