16S rDNA Amplicon Sequencing of Anaerobic bioreactors degrading oleic acid- Day 21 Samples
DNA samples were collected from triplicate bioreactors fed oleic acid on day 21 of operation. Samples were processed with nested two-step PCR on the bacterial 16S rRNA gene. PCR products were purified and submitted for deep-sequencing on Illumina MiSeq platform.