GSM4676855: KO4; Mycobacterium tuberculosis; RNA-Seq
Isolation of RNA was performed using TRIzol reagent (Ambion). Cells equivalent to 10 O.D. of Rv, Rv∆embR, and Rv∆embR::embRstrains were harvested and resuspended in TRIzol reagent. The cells were lysed using 0.1 mm zirconium beads, following which RNA was extracted through chloroform, precipitated, and washed using 70% Ethanol (prepared in DEPC-water). The RNA was resuspended in the nuclease-free water and further purified using the RNeasy mini kit (Qiagen). The samples were treated with DNase I to remove any leftover DNA traces. TruSeq RNA Library Prep Kit v2