RNA shotgun metagenomics pooled Aedes mosquitoes
All samples were pooled for library construction. The NuGEN Universal RNA protocol was run with and without depletions. Depletions included rRNA removal from 200 ng of total RNA using the RiboZeroTM Magnetic Gold kit (Illumina, San Diego, California, USA) following the manufacturer instructions. Depletion of the highly abundant A, aegypti transcripts followed, using custom AnyDeplete probes (Tecan Group Ltd)