GSM1895369: HS 2; Homo sapiens; RNA-Seq
Total RNA was purified using the RNA MicroPrep Kit (Zymo Research). Complementary DNA (cDNA) was generated using SuperScript II Reverse Transcriptase (Invitrogen) according to the manufacturer’s instructions. mRNA was reverse transcribed with random hexamers. Library preparations were performed with the TrueSeq RNA Sample Preparation Kit (Illumina) and sequencing was performed on the Illumina HighSeq 2500 with 50bp paired end sequencings.