GSM2671842: ID-seq cell-dilution; Homo sapiens; OTHER
DNA-tags were released from immuno-stained cell populations using 1.5 hour DTT treatment. Released DNA-tags were used in an overhang PCR reaction that adds sample-specific barcode sequence. Then a pool of these barcoded DNA-tags was used for a second PCR to add Illumina adaptor and index sequence to the DNA fragments.