GSM2572813: Mili-ip; Mus musculus; ncRNA-Seq
Total cellular RNA was extracted using TRIzol reagent (Takara). 4 μg total RNAs with 2 X 10-6 pmol spike-ins mixture from wild type SSC and mhen1 knock-out SSC were treated by 2 μl freshly dissolved NaIO4 (200 mM), kept on ice for 1 hr in the dark. Then the RNAs were precipitated and dissolved in DEPC-treated water for small RNA library construction. 0.5 μg of RNA with or without NaIO4 treatment was used to construct a small RNA library according to the Illumina protocol. High-throughput RNA sequencing was performed using Hi-Seq2500 with 50 running circles.