GSM4188529: TAM_AP rep2; Mus musculus; RNA-Seq
The anterior and posterior portions of the palatal shelves were dissected out from E13.5 TAM-treated mice and controls (n = 3 for each group), respectively, and subjected to total RNA extraction (RNeasy Micro Kit, cat. 74004; Qiagen). The quality and quantity of RNAs were determined by using a Qubit 2.0 Fluorometric Quantitation system (Life Technologies). Finally, the RNAs were subjected to RNA-Seq analysis. Gene Ontology and pathway categories were grouped according to functional theme and proportion of cases that were affected by individual pathway alterations was plotted per subgroup, and across series. RNA libraries were prepared for sequencing using standard Illumina protocols