GSM3320028: 471 1st generationp53 Mut MSC TL (3); Mus musculus; RNA-Seq
RNA extraction by The Direct-zol™ RNA Kit from ZYMO Total RNA was fragmented followed by reverse transcription and second strand cDNA synthesis. The double strand cDNA was subjected to end repair, A base addition, adapter ligation and PCR amplification to create libraries. Libraries were evaluated by Qubit and TapeStation. Sequencing libraries were constructed with barcodes to allow multiplexing of 22 samples on a 2 lane of Illumina HiSeq 2500 V4 instrument, resulting in ~18 million single-end 60-bp reads per sample.