GSM3318682: Group H, Cow 5, post; Bos taurus; RNA-Seq
Peripheral blood mononuclear cells (PBMC) were separated from veinous blood by Ficoll density gradient. Total RNA was isolated with Trizol, with an extra chloroform extraction to remove residual phenol and addition of glyco-blue as a carrier to promote RNA precipitation. Diretional RNA-seq libraries were prepared from 500ng total RNA using the NEBNext Directional Ultra II RNA Library Prep Kit for Illumina (New England Biolabs), with initial polyA+ isolation.