GSM4675962: R201Q_NoDox; Mus musculus; ATAC-seq
ATAC-seq was performed essentially as described (Buenrostro et al, 2015), briefly, 50,000 KIT+ CD41+ Tie2- Progs were transposed in 1x Tagment DNA buffer (Illumina), Tn5 transposase (Illumina) and 0.01% Digitonin (Promega) for 30 minutes at 37°C with agitation. DNA was amplified by PCR using Nextera primers and libraries were sequenced using a Next-Seq 500 high output kit v2 75 cycles (Illumina).