Whole-genome sequencing of Mycobacterium tuberculosis: human sputum samples
Colonies from LJ media were used for DNA extraction. Genomic libraries were constructed using Nextera XT DNA Library Prep kit following the manufacturer's instructions. Individual libraries were indexed with Nextera XT Index Kit. Paired end reads from all isolates were obtained using a whole-genome shotgun strategy with an Illumina MiSeq at the Instituto Malbrn, Buenos Aires, Argentina.