LncRNA-Seq of Jatropha curcasIntermediate seeds
RNA was fragmented. The RNA fragments then served as a template for first strand cDNA synthesis using random hexamers and reverse transcriptase. Second strand cDNA was synthesized using DNA polymerase I and RNaseH, and then purified using an AMPure XP beads (Agencourt). The cDNA was obtained by agarose gel electrophoresis and amplified by PCR to construct the final cDNA library for paired-end sequencing using Illumina HiSeq2500 platform