GSM3553701: S15 ControlBMDM_LPSstimulated_rep1; Mus musculus; RNA-Seq
Cells were lysed and RNA isolated using mirVana mRNA/miRNA isolation kit (ThermoFisher Scientific, UK), according to manufacturer's instructions. RNA-seq libraries were prepared with the TruSeq Stranded mRNA LT sample preparation kit (Illumina, UK) using both Sciclone and Zephyr liquid handling robotics (PerkinElmer, UK). Library concentrations were quantified with the Qubit Fluoro-metric Quantitation system (Life Technologies, UK) and the size distribution was assessed using the Experion Automated Electrophoresis System (Bio-Rad, USA).