Tentacle transcriptome of the Pacific sea nettle, Chrysaora fuscescens
The fishing tentacles of one C. fuscescens specimen were manually excised, cut into pieces (5 cm long) and flash-frozen in dry ice. Tentacle samples were shipped to LC Sciences (Texas, United States) for RNA extractions and Next Generation Sequencing. Total RNA was extracted using the RNeasy Mini Kit (Qiagen) as specified by the manufacturer and the integrity was assessed using an Agilent RNA 6000 Nano chip and the Agilent Bioanalyzer 2100 system (Agilent Technologies). High-quality total RNA was then used to construct a cDNA library using the TruSeq stranded mRNA Sample Prep Kit (Illumina) and sequenced using massively parallel synthesis in one lane on an Illumina HiSeq 2000 system.