Illumina HiSeq 2500 paired end sequencing; human SU-DHL-5 and TMD-8 B-lymphome cell line subclones
human SU-DHL-5 and TMD-8 B-lymphome cell line subclones
Cell lines were FACS-sorted and single cell clones were picked and phenotyped. Growth of sorted cell clones was observed over a four-week period by microscopy. Fragmentation of 100 ng purified genomic DNA (gDNA) in 55 l Tris-EDTA buffer was done on Covaris S2. Fragmentation of 100 ng purified genomic DNA (gDNA) in 55 l Tris-EDTA buffer was done on Covaris S2. After library preparation from 100 ng of fragmented gDNA using Agilent SureSelectXT v5+UTR (75 Mb), libraries were purified, size validated and prepared for sequencing according to the manufacturer's protocols. Libraries were sequenced on Illumina HiSeq2500 using TruSeq SBS Kit v3-HS (2x101, paired-end run).