ITS sequencing of decomposing leaf litter  samples: longterm Global Change Experiment
A longterm global change field experiment  site was used to collect leaf litter samples. The samples were collected from  ambient or reduced water plots and from ambient or added nitrogen plots. Leaf  litter DNA was isolated and fungal communities were targeted with the Looby  et.al. primers. The PCR products were  sequenced on the Illumina miseq platform using the v3 PE300.