Whole genome sequencing of Plasmodium falciparum
Genomic DNA (gDNA) was generated from a cryovial of the P. falciparum NF135.C10 strains cell bank, with identifier SAN07-010410. Culture was expanded according to a standard protocol (Trager and Jensen 1976. Science). gDNA was extracted with Qiagen lood DNA Midi Kit. The Oxford Nanopore library was constructed with SQK-RAD004 and sequenced on a FLO-MIN106 R9 according to the manufacturers protocol except that sequencing beads were replaced with water.