RNA-seq of embryo sac pericarp
Greenhouse-grown pre-fertilized maize B73 ears were fixed in Farmers fixative, cleared in butanol and embedded in paraplast. Serial microtome sections (10um thick) of ovules were transferred onto Leica PEN (polyethylene naphthalate) laser microdissection slides. Ovule cell type-specific sections were captured using a Leica LMD6500 laser microdissection microscope. Total RNA was isolated from tissue sections using the Arcturus Pico Pure RNA Isolation kit. The SMARTer cDNA Synthesis Total RNA-Seq Kit v2-Pico Input Mammalian was used starting with 10ng total RNA to generate Illumina-compatible indexed RNA-seq libraries which were sent to Novogene for sequencing.