GSM1896840: Co-culture replicate 3, 4 hours after heterotroph addition; Alteromonas macleodii; Prochlorococcus marinus str. NATL2A; RNA-Seq
Culture samples added to cold RNAlater at each timepoint, cells collected onto a 0.2µm filter, and total RNA extracted with the mirVana kit (Ambion) Strand-specific RNAseq library construction starting with 100ng of total RNA using the dUTP second-strand approach; duplex-specific normalization was performed to reduce ribosomal RNA content in each sample