Transcriptome of the hepatopancreas of the precocious juvenile crabs
Precocious and normal juvenile crabs were randomly sampled in a pond in March 2014. The normal mature crabs were randomly sampled in the same pond in December 2014. All samples from each group were anaesthetized on ice and dissected separately to collect the hepatopancreas (6 crabs for each group).Total RNA were extracted from the hepatopancreas tissues of each crab in each group with TRIzol Reagent (Invitrogen, Shanghai, China).Equal amounts of RNA from every crab within each group were pooled together for the de novo sequencing to generate a reference transcriptome.