RNA-Seq of hy5
Total RNA was extracted from roots of 15-d-old seedling of WT-illuminated, hy5-illuminated, hy5-covered, and WT-covered under the root-covered system, respectively. After purification, RNA integrity was verified using the RNA 6000 Nano Labchip Kit on an Agilent 2100 Bioanalyzer (Agilent Technologies) following the manufacturers protocol. Enrichment of mRNA from the total RNA, cDNA synthesis, and construction of the library were performed by the Beijing Genome Institute (BGI, China). The Illumina HiSeqTM 2000 sequencing system was used to sequence the libraries.