ATAC-Seq of WT (KOS) and ICP4  null (n12) HSV-1 Productive Infection in MRC5 cells
MRC5 cells were mock-infected or  infected with WT (KOS) or ICP4-null (n12) HSV-1 at an MOI of 10 PFU/cell. WT  samples were harvested after two hours. Mock and n12 infected cells were  harvested after four hours. ATAC-Seq was performed on 1E+6 cells which were  isolated and tagmented using the Nextera DNA library prep kit. Illumina  sequencing was carried out at Tufts University Core Facility using HiSeq 2500  to generate 50 bp single-end reads.