GSM2753320: E12.5 WT MALE GUT REP1; Mus musculus; RNA-Seq
Guts were dissected out in cold leibovitz medium and RNA was extracted and cleaned using a Trizol Rneasy column hybrid method cDNA  was prepared  by oligo dT beads to select mRNA from the total RNA sample followed by heat fragmentation and cDNA synthesis from the RNA template as part of the Illumina Tru Seq™ RNA Sample Preparation protocol. The resultant cDNA then was used for library preparation (end repair, base ‘A’ addition, adapter ligation, and enrichment) using standard Illumina protocols.