GSM3690828: KO brown fat 1; Mus musculus; RNA-Seq
Mature brown fat is isolated from minced adipose tissues which were digested with collagenase A. The mature adipocytes was obtaibed in the upper lipids layer after centrifugation at 700 g for 5 min. RNA were extracted using QIazol reagent and RNAeasy kit (Qiagen.) mRNA library was prepared using KAPA Hyper mRNA kit