RNA-Seq
Cold stress was performed on these samples by transferring 28-d-old seedlings (three-leaf stage) to a growth chamber set to 4 oC. Crown tissues (about 1 cm in length) were taken at 0 h (control), 3 h, 24 h, and 5 d after cold treatments. The sequencing library construction and sequencing were performed in the Beijing Genomics Institute (BGI)-Shenzhen, Shenzhen, China (http://www.genomics.cn/index.php) with Illumina HiSeq 4000 (Illumina Inc., USA). In total, 48 libraries with a 100 bp single-end reads were constructed and sequencing of the purified libraries were carried out on a Hiseq 4000.