GSM2492575: PB naïve CD8SP R1 beta; Homo sapiens; RNA-Seq
Cells were lysed in RLT buffer and RNA was extracted using Qiagen RNEasy Micro kit per manufacturer's protocol A focused TCRαβ cDNA library was prepared using the Clontech SMARTer PCR cDNA synthesis kit according to a gene-specific modification of the manufacturer's protocols. cDNA was reamplified with a semi-nested strategy to introduce sample barcodes and 8 barcoded sample libraries (4 alpha, 4 beta) were pooled prior to Illumina adaptor ligation.