Illumina sequencing of Pinus halepensis transcriptome
RNA-Seq libraries were generated using the TruSeq RNA-Seq Sample Prep kit according to the manufacturer's protocol (Illumina Inc., San Diego, CA). Briefly, poly-A RNA was isolated from total RNA and chemically fragmented. First and second strand synthesis were followed by end repair, and adenosines were added to the 3' ends. Adapters were ligated to the cDNA and 200 * 25 bp fragments were gel purified and enriched by PCR. The library was quantified using Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA) and sequenced on the Illumina HiSeq2000 (Illumina Inc., San Diego, CA) as paired-end reads of length 101 bp.