GSM2411272: RNA sequencing of set25 mutant worms biological replicate 1; Caenorhabditis elegans; RNA-Seq
RNA was extracted using TRIzol® extraction protocol (Portman, D.S. (2006). Profiling C. elegans gene expression with DNA microarrays. WormBook : the online review of C elegans biology, 1-11.) Libraries for RNA-seq were prepared from 400 ng total RNA using the TruSeq Stranded Total RNA Library Prep Kit (ref. RS-122-2201, Illumina) using the included RiboZero reagents to deplete ribosomal RNAs according to manufacturer's instructions. The poly(A)-mRNA selection step was not carried out. Samples were sequenced with 2x50 bp paired-end reads using Illumina HiSeq v4 sequencing chemistry.