GSM1625699: Ak2; Mus musculus; RNA-Seq
RNA was isolated from fresh heart tissue using mirVana kit. RNA libraries were prepared for sequencing using TruSeq RNA sample prepration kit from Illumina. 1μg of RNA was used to construct the library. QC was done by bioanalyzer and quantified by Qubit. 6PM strating quantity was used for further sequencing. 100 base single read run after o-botclustering was performed on Illumina HiSeq 2500.