GSM2753293: 3-weeks-1; Mus musculus; RNA-Seq
Total RNA was isolated from liver samples using Trizol reagent (Life Technologies, Paisley, UK) and purified using MaXtract High Density (QIAGEN, Redwood City, CA). RNA yield and quantity was determined using a Nanodrop spectrophotometer ND-1000 (Thermo scientific, Wilmington, DE). The RNA quality was tested using a ND-1000 V3.7.1, according to the manufacturer’s instructions and assigned an RNA integrity number (RIN). Only samples with a RNA integrity number (RIN) of 8 or greater were employed. Each sample represents equal amount of total RNA from three mice that were pooled prior to library construction. RNAseq libraries were constructed in the center for Genomic Technologies using Trueseq RNA Library preparation kit according to Illumina protocol and sequenced with the Illumina Nextseq 500 System to an average depth of around 30 million reads.