GSM3553511: MKO_4wk_repB; Mus musculus; RNA-Seq
Total RNA was isolated using PureZolTM RNA Isolation Reagent (BioRad Laboratories) and treated with DNase according to the manufacture's protocol. Total RNA quality was evaluated using the Agilent Bioanalyzer and the RNA 6000 Nano kit. Only samples with a RIN score ≥ 8 were processed further. RNA-seq libraries were generated from 1 µg of RNA using the TruSeq Stranded Total RNA Sample Prep Kit with Ribo-Zero ribosomal RNA reduction chemistry (Illumina). Electrophoresis of the libraries on a Bioanalyzer 2100 instrument showed highest peaks at 230-270 bp. Paired-end multiplexed sequencing of libraries (four per flow cell lane) to generate reads of 100 bp was performed on a HiSeq 1000 instrument with TruSeq SBS and PE Cluster v3 Kits (Illumina). 70-100 million reads were obtained for all samples. Paired end sequence reads (100-bp in length) were generated on an Illumina HiSeq 1000 (Sample 1 to 8)