GSM2068337: Wildtype RNA pol II ChIP rep1; Neurospora crassa; ChIP-Seq
Cells were treated with 1% formaldahyde and resuspended in ChIP lysis buffer. Cells were disrupted by sonication and immunoprecipitation was performed with the indicated antibody. Libraries were constructed using the Illuina TruSeq ChIP-seq libarary according to manufacturer instructions except that only 4 cycles of PRC were performed to limit bias against A:T sequences