GSM4224843: Control, sample 7 male; Mus musculus; RNA-Seq
RNA was isolated from the left hippocampus using the AllPrepâ DNA/RNA/Protein Mini Kit (QIAGEN, Cat# Cat# 80004) per manufacturer's instructions and stored at -80°C. Total RNA samples were quantified and checked for quality (all RINs>9.0) using the 2100 Bioanalyzer on a RNA 6000 Nano Assay (Agilent Technologies, Inc). Samples concentrations were normalized to 500ng total RNA and libraries were constructed using the KAPA mRNA HyperPrep Kit for Illumina Platforms (KAPA Biosystems) according to the manufacture's protocol using 9 PCR cycles for library amplification. cDNA libraries were validated using the Bioanalyzer 2100 on a High Sensitivity DNA assay. Quantification was done using the KAPA Library Quantification Kit for Illumina Platforms on a Roche Lightcycler 480.  Sequencing was done on an Illumina NovaSeq 6000 using the S4 XP workflow 2x100 with each library using 1.25% of the S4 lane.