GSM4564167: BIN67_Control_ATACseq_Rep1; Homo sapiens; ATAC-seq
Four biological replicates were processed for ATAC-seq following standard protocol (Buenrostro, J. D., et al, (2013) Nature Methods ) with modifications:  increasing the cell number per sample to 100,000 cells, the use of Zymo ChiP DNA Clean & Concentrator Kit (Zymo Cat #D5205) in place of Qiagen MinElute, and lastly size selected with AMPure XP Beads (1.8x volume; Agencourt #A63880). For each biological replicate, samples were done in technical triplicate and run on 6% TBE gel to assay for nucleosome banding. The technical sample with the most pronounced nucleosome banding within each biological sample was selected for future studies and were then analyzed by Agilent Tapestation for molarity content. Samples were equimolar pooled paired end sequenced across two lanes on a Illuminia HiSeq2500 (Paired end x 50 bp). Libraries were prepared following standard protocol (Buenrostro, J. D., et al, (2013) Nature Methods)