GSM4186355: FF_H3K27Ac; Mus musculus; ChIP-Seq
Frozen tissue was histologically examined and sectioned. Sample was crosslinked for 10 minutes at room temperature with 1% formaldehyde, lysed and sonicated. Solubilized chromatin was immunoprecipitated following a standard ChIP-seq protocol. Libraries were prepared with Accel-NGS 2S Plus DNA Library Kit (Swift Biosciences) or SMARTer® ThruPLEX® DNA-Seq Kit (Takara Bio USA Inc).