GSM2410404: HepG2_shIGF2BP3_3h_rep1; Homo sapiens; RNA-Seq
The total RNA was extracted by miRNeasy Kit (Qiagen). For RNA sequencing, equal amount of ERCC RNA spike-in control (Thermo Fisher Scientific) was added to the total RNA samples as internal controls before library construction. Sequencing libraries were prepared using NEBNext Ultra Directional RNA Library Prep Kit.