GSM4189589: 2-cell-siEtv5-2; Mus musculus; RNA-Seq
Harvested blastomeres were placed into lysis buffer, and Superscript II-triggered reverse transcription followed by cDNA amplification were performed. The amplified cDNA was then fragmented using a Covaris sonicator. The fragmented DNA were subjected to library construction using KAPA Hyper Prep Kit for the Illumina platform, following the manufacturer's instructions.