GSM4186360: FiT-seq_H3K4me2; Mus musculus; ChIP-Seq
FFPE block was histologically examined and sectioned. Samples were deparaffinized, rehydrated and homogenized by proteinase K treatment and sonication in 1% SDS detergent buffer. Solubilized chromatin was immunoprecipitated following a low-input protocol. Libraries were prepared with Accel-NGS 2S Plus DNA Library Kit (Swift Biosciences) or SMARTer® ThruPLEX® DNA-Seq Kit (Takara Bio USA Inc).