GSM2068353: Wildtype Mnase-Seq single-end; Neurospora crassa; MNase-Seq
Cells were treated with 1% formaldahyde and resuspended in NPS buffer. Chromatin was release by treatment with Micrococcal nuclease. DNA was then run on a gel and band corresponding to mononuclesomes were extracted and used to make libraries. Libraries were constructed using the Illuina TruSeq ChIP-seq libarary according to manufacturer instructions except that only 4 cycles of PRC were performed to limit bias against A:T sequences