GSM4676072: 21dpi untreated rep2; Mycobacterium tuberculosis H37Rv; Tn-Seq
Spleens were isolated and homogenized. Approximately one million CFU per mouse were plated on 7H10 agar with kanamycin (10µg/ml). Genomic DNA was extracted as  described in Long JE, DeJesus M, Ward D, Baker RE, Ioerger T, Sassetti CM. 2015. Identifying essential genes in Mycobacterium tuberculosis by global phenotypic profiling. Methods Mol Biol 1279:79-95. Libraries were prepared for Illumina sequencing using custom primers as described in Long JE, DeJesus M, Ward D, Baker RE, Ioerger T, Sassetti CM. 2015. Identifying essential genes in Mycobacterium tuberculosis by global phenotypic profiling. Methods Mol Biol 1279:79-95.