GSM2393238: A. prostrata leaf mature, primary branch, middle, rep 3; Atriplex prostrata; RNA-Seq
Leaves, stem, and roots were harvested and immediately frozen in liquid nitrogen. RNA was isolated from ground tissue using the Qiagen RNeasy plant mini kit. Residues of DNA were removed with DNase (New England Biolabs). RNA integrity, sequencing library and fragment size were analyzed on a 2100 Bioanalyzer (Agilent). Libraries were prepared using the TruSeq RNA Sample Prep Kit v2 (Illumina), and quantified with a Qubit 2.0 (Invitrogen). Libraries were prepared using the TruSeq RNA Sample Prep Kit v2 (Illumina). Samples were multiplexed with 12 libraries per lane and sequenced in single-end (100 bp read length) or paired-end mode (100 bp or 150 bp read length) on an Illumina HiSeq platform.