RNA-Seq of mdx mus musculus with mild peripheral nerve injury: young male, isolated muscle stem cell
Briefly, total RNA was isolated from sorted cells using Trizol (Thermo)  and the miRNeasy Micro Kit (Qiagen) as per the manufacturers instructions and  RNA concentration and integrity were measured with a Nanodrop  spectrophotometer (Nanodrop 2000c) and Bioanalyzer (Agilent 2100).  Approximately 10ng of isolated total RNA was used to produce cDNA libraries  using the SmartSeq4 protocol (Clontech), as per the manufacturers  instructions. Individual libraries were pooled and sequenced using 76-bp  paired-end reads on an Illumina NextSeq to an average depth of 50M reads per  library.