GSM2573855: E10.5_DPR_Pdx1-low_6_18; Mus musculus; RNA-Seq
Bulk-cell RNA-seq (Sample 001-016) For E10.5, E14.5 and E17.5 dorsal and ventral pancreases, a total of 5-7 x 10e4 sorted cells were used to isolate total RNA using the Rneasy Micro Kit (Qiagen, 74004). Poly(A) RNA was further purified with the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490). Libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB #E7420) according to the manufacturer's instructions. Single-cell RNA-seq (Sample 023-541, 568-586, 607-631, 671-674) Libraries were prepared according the Smart-seq2 method as previously reported (Picelli et al., 2014). 2 ng cDNA were subjected to prepare the libraries using the TruePrep DNA Library Prep Kit (TD502, Vazyme).