lncRNA-Seq of Homo sapiens: adult cardiac mitochondria
NA, isolated from human atrial appendages and mouse whole heart (as described above), was sequenced through the West Virginia University Genomics Core Facility. Briefly, RNA was selected for long non-coding RNA analysis using a Ribo-Zero rRNA Removal Kit (Illumina, San Diego, CA). Samples were run on the HiSeq (Illumina) in 51bp paired end reads achieving ~30 million clustered reads per sample. Generated Fastq files were processed through HISAT2 under standard parameters, with the exception of quality scoring control specified under --phred33. The reference genome used in generating BAM files included fasta formatted DNA from Ensemble release 95 for human (GRCh38) and mouse (GRCm38).