GSM4564440: DMSO rep2; Arabidopsis thaliana; RNA-Seq
Seedlings of 3 biological replicates for each treatment were harvested in liquid nitrogen and total RNA was isolated with a RNeasy mini kit (Qiagen) as per manufacturer instructions, quantified with a nanodrop (ND-1000) and treated with TURBO DNA-free kit (Ambion) to remove contaminating genomic DNA RNA quality was determined prior to library construction using an Agilent Bioanalzyer and RIN scores of 9.5 -10 was obtained for all samples. cDNA libraries were prepared using the Illumina's TruSeq RNA sample preparation kit (Illumina) according to manufacturer's instructions.