GSM2068806: myb-GFP_9h_rep_1; Xenopus laevis; ChIP-Seq
Embryos were fixed in 1% paraformaldehyde in PBS for 30 minutes at 22 degrees and fix was quenched with 0.125M glycine. ChIPseq was performed as described (PMID: 24934224) with the following antibodies: GFP (Invitrogen catalog no. A11122, lot no. 1296649), FLAG (Sigma, cat #F1804), H3K4me3 (Active Motif, cat #39159; lot #01609004), H3K27ac (Abcam, cat #ab4729, lot #GR71158-2), or rad21 (Abcam, cat #ab992). Following sonication and immunoprecipitation of genomic fragments, we polished and adenylated ends (NEB End-It and A-tailing modules) and ligated Truseq v2 adapters (Illumina).