GSM2412748: High BC 1; Clostridium autoethanogenum; RNA-Seq
Frozen pellets were thawed and total RNA extracted using two cycles of cellular lysis in RNase-free zirconia beads (Ambion). RNA was purified using the RNeasy mini kit (Qiagen) with off-column DNase treatment followed by enrichment and purification using RNA Clean & ConcentratorTM (Zymo Research). Next, ribosomal RNA was removed using the Ribo-Zero rRNA removal kit (Illumina). mRNA libraries were prepared using standard TruSeq library preparation protocols (Illumina).