GSM4072788: Input HMR+LHR induced rep2; Drosophila melanogaster; ChIP-Seq
ChiP is prefromed exactly as described in Gerland et al, 2017. Shortly, the cells were crosslinked in 1% FA for 5 min at room temperature and frozen. Chromatin from 50 million cells was sheared in TE/0.1%SDS, the buffer was adjusted to RIPA and after preclearing the sheared extract and taking 1/10 as an input, IP was done overnight. Beads were washed, after which RNA/protein digestion and decrosslinking was performed. DNA was purified using AMPure XP beads. Libraries were prepared from 1 ng DNA with Microplex Diagenode kit, according to manufacture's instructions, without size selection.