Whole genome sequencing of B. cellulosilyticus WH2 using the PacBio RSII platform
High molecular weight genomic DNA was extracted from an overnight culture using a hexadecyltrimethylammonium bromide (CTAB)/Chloroform-Isoamyl alcohol genomic DNA extraction protocol and then sheared to 10-20 kb fragments using Covaris g-TUBES. Sequencing libraries were prepared with 5 µg of the sheared DNA according to the “20 kb Template Preparation with 15kb Cutoff Using BluePippin™ Size-Selection” protocol (https://pacbio.secure.force.com/Share/Protocol?id=a1q70000004GlScAAK) and run on the Pacific Biosciences RSII sequencer with 180 minute movies using P4/C2 chemistry.