miRNAseq of  root
Small RNA libraries were prepared using isolated total RNA according to the protocol of Illumina TrueSeq Small RNA Library Preparation Kit (https://support.illumina.com/). The protocol includes adapter ligation, reverse transcription, PCR amplification, and pooled gel purification. The gel size selected and purified libraries were analyzed in Agilent 4200 Tape station system (Agilent Technologies) using high sensitivity D1000 Screen tape as per manufacturers instruction. After obtaining the Qubit concentration for the libraries and the mean peak size from the Agilent tape station profile, the SE Illumina Libraries were loaded onto NextSeq 500 for cluster generation and sequencing.