GSM2413190: EE_1; Mus musculus; RNA-Seq
RNA was isolated using Ambion RNAqueous Kit. RNA was amplified using Illumina TruSeq Stranded RNA Kit w/ Ribo-Zero Gold, and sequencing was performed on Illumina HiSeq using 50 single-cycle end read. negative binomial distribution (Love et al., 2014). All analyses were quality controlled and coordinated via USeq, an open-source suite of sequencing tools developed by the University of Utah’s Bioinformatics Shared Resource (http://useq.sourceforge.net; (Nix et al., 2008)).