GSM1520902: Mitoplast RNA; Mus musculus; RNA-Seq
Mitoplasts were extracted using Trizol Libraries were prepared at the HudsonAlpha Genomic Services Lab. RNA was first amplified using an Ovation RNA-seq kit (NuGEN, San Carlos CA) to amplify the RNA and produce a cDNA library. cDNA was electrophoretically separated and gel excision allowed selection of fragments roughly 300 nucleotides in length. The cDNA library was then sequenced.