GSM4186895: Dnmt1_null_2; Mus musculus; RNA-Seq
Total RNA was extracted and traces of contaminating genomic DNA were eliminated by two successive treatments with DNAse (Turbo DNase, Ambion). The integrity of the RNA was veri-fied using the Bioanalyzer RNA 2100 Nano Assay (Agilent Technologies). RNA-seq libraries were prepared with the TruSeq RNA Sample Prep Kit v2 (Illumina).