ITS amplicons of ectomycorrizal fungal root tips: Spatial heterogeneity experiment,Inoculum soil
25ml of soil was collected and sieved. DNA was extracted following the manufacturer's instructions for Powersoil DNA tubes  . Samples were PCRed for the ITS1 region with ITS1F and ITS2 primer pair. PCR products were cleaned with AMpure beads, quantified with qubit, and pooled to equimolar concentration before shipping to be sequenced at the Genomics Sequencing Laboratory, University of California, Berkeley, CA, USA.