vNHI-VP0614-14
Total RNA was extracted from cell culture supernatant using TRIzolTM LS Reagent (Life Technologies) following manufacturer's instructions. Double stranded complementary DNA (ds-cDNA) libraries were prepared with the Ion Total RNA-Seq Kit V2 (Life Technologies). cDNA libraries were quality-checked with Agilent 2100 Bioanalyzer (Agilent High Sensitivity DNA kit, Agilent Technologies), quantified by qPCR  and sequenced on the Ion ProtonTM Sequencer using an Ion PITM Chip Kit v2 (Life Technologies).