GSM2491691: WT-25dpa_BS; Solanum lycopersicum; Bisulfite-Seq
Tomato genomic DNA was extracted using a modified cetyltrimethylammonium bromide-based method (Clarke, 2009) or by using the QIAGEN DNeasy Plant Maxi Kit following the prodcut manual. Totol RNA were extracted using Trizol reagent (Ambion, 15596) Genomic DNA was extracted from the tomato fruit and sent to Genomics Core Facility of Shanghai Center for Plant Stress Biology for bisulfite treatment, library preparation, and sequencing. Bisulfite sequencing libraries were prepared using standard Illumina protocols. Total RNA were extracted from the tomato fruit using methods described above. Library construction and deep sequencing were performed by the Core Facility of Genomics in Shanghai Plant Stress Biology Center, China.