GSM4563978: Time_0_rep3; Laetiporus sulphureus; RNA-Seq
The mycelium was ground with liquid nitrogen, and total RNA extraction was performed with mirVana™ Total Isolation Kit (Thermo Fisher), according to the manufacturer's instructions. The resulting solution was treated with DNAse (DNA-Free RNA Kit, Zymo Research), purified with RNeasy Kit (Qiagen) and quality was verified using RNAnano Bioanalyzer 2100 chip (Agilent). Libraries were prepared using Illumina TruSeq mRNA kit according to the manufacturer's instructions and sequenced on the Illumina HiSeq 2500 platform.