RNA-seq of Homo sapiens: adenoid cystic carcinoma tumor: Sample case67
RNA-SEQ using RNA isolated from FFPE tissue slices. RNA was extracted using the Qiagen RNAeasy kit, after DNase I treatment and rRNA depletion (Clontech RiboGone Mammalian kit), cDNA was made using the Clontech SMARTer Universal Low Input RNA kit and the library was prepared using the Ion Plus Fragment Library kit from Life Technologies. All cleanup steps were performed using Agencourt Ampure XP beads. Emulsion and enrichment steps were done on the Ion Chef, and the samples were sequenced on an Ion Torrent Proton, using P1v2 chips, in the ATG facility at the University of New Mexico. Libraries were multiplexed (generally 4 samples).