smallRNA-Seq of Homo sapiens: Cardiac mitochondrial ND and T2DM
RNA isolated from human atrial appendages and mouse whole heart (as described above) was sequenced through the West Virginia University Genomics Core Facility. Small RNA was selected using the NEXTFLEX Small RNA-Seq Kit v3 (Bioo Scientific, Austin, TX). Samples were run on the HiSeq in Rapid Run (Illumina) in 64bp single end reads on two lanes achieving ~12 million clustered reads per sample. Adapter trimming through cutadapt (5- TGGAATTCTCGGGTGCCAAGG -3) allowed for isolation of genomic reads. Generated Fastq files were processed through Bowtie using the parameters, -a -m 3 --best --strata --chunkmbs 250. The reference genome used in generating BAM files included fasta formatted DNA from Ensemble release 95 for human (GRCh38).