GSM3933476: H3K36me3 ChIP-seq of SH881; Oryza sativa; ChIP-Seq
Fresh leaf blades of 40 d seedlings of early-maturing NIL D248 (BC5F11) and its recurrent parent SH881 (Shuhui881) were harvested for fixation. Chromatin was isolated and sonicated to generate DNA fragment with an average size of about 500 bp. The solubilized chromatin was immunoprecipitated by Protein A beads (Millipore) with anti-H3K36me3 antibody (Abcam, ab9050), and the co-immunoprecipitated DNA was recovered and analyzed by ChIP-seq. More than 10 ng ChIP DNA each sample was used for Illumina library generation following the manufacturer's instructions.