GSM4072660: PN251_passage_5; Homo sapiens; RNA-Seq
RNA was extracted from cells using the Rneasy Plus Mini Kit (Qiagen) according to manufacturers instructions. Ribosomal RNA depletion was performed using Ribo-Zero Kit (New England Biolabs). RNA-seq libraries were prepared using the NEB Next Ultra Directional RNA Library Kit (New England Biolabs).