GSM3860782: Bulk RNA sequencing MPP1 WT 3; Mus musculus; RNA-Seq
3 replicates (from 3 different mice) of LK, HSC, MPP1, MPP2 and MPP4 cells were sorted from Mettl3 f/f and Mettl3 cKO mice base on cell surface marker. For bulk sequencing, RNA was extracted from cells using the SMARTer RNA extraction method for minimal cells RNA extraction. After amplification, cDNA was subjected to automated Illumina paired-end library construction. Libraries were sequenced on Illumina HiSeq2000 instruments with paired reads of 50 bp in length per sample.