GSM1523662: ESC RNA-Seq; Mus musculus; RNA-Seq
Library was created using SOLID Whole Transcriptome Analysis Kit (Life Technologies). Briefly, poly(A) selected RNA was treated with RNAse III. Fragmented RNA were hybridized and ligated with adaptors. This is followed by a reverse transcription. cDNA was purified and subjected to a size selection on a PAGE gel. Fraction 175-250bp was excised and purified. Amplification was performed using PCR primers adapted for Illumina sequencing for 15 cycles. Trizol RNA extraction