GSM4224103: dcl2dcl3dcl4 replicate3; Arabidopsis thaliana; RNA-Seq
Buffer containing 0.1 M NaCl, 2% SDS, 50 mMTris–HCl (pH 9), 10 mMEDTA (pH 8) and 20 mM ß- mercaptoethanol was added to the frozen powder andRNA was extracted twice with phenol and recovered by ethanol precipitation. For small RNA analysis, total RNA was sep- arated on a 15% denaturing polyacrylamide gel and stained with ethidium bromide. Library construction has been realized by GATC Biotech All libraries were pooled together and sequenced on HiSeq 2000 platform