GSM1521277: N-67; Homo sapiens; miRNA-Seq
Fresh-frozen lung adenocarcinoma (LUAC) tumor and patient matched non-malignant lung parenchymal tissues were collected from treatment naive patients; Tissue microdissection was guided by a lung pathologist to ensure >80% tumor cell or >80% non-malignant cell content. Total RNA was extracted using Trizol reagent Full description of library construction, sequencing, read pre-processing, alignment and annotation are previously described [TCGA: Comprehensive molecular portraits of human breast tumours. Nature 2012, 490(7418):61-70]