mouse inner ear surface epithilium miRNA - Cochlea
miRNA libraries for MPS were created using the TruSeq SmallRNA SamplePrep Kit (Illumina), with one modification. Instead of running the samples on acrylamide gel in order to isolate the miRNA band, the samples were loaded on a 4% agarose E-Gel and the purification was performed with the Qiagen Kit. The libraries were multiplexed and 2.5 pM of each library was sequenced at SR x 36 bp using the Illumina Genome Analyzer IIx in the same lane at the Functional Genomics Laboratory at Tel Aviv University.