GSM4501360: siB/siM-3; Homo sapiens; RNA-Seq
RNA was isolated using the Rneasy Plus Mini Kit (Qiagen) and processed as per manufacturer's instructions. Illumina TruSeq Stranded Total RNA kit with Ribo Zero Gold for rRNA removal was used. Illumina TruSeq libraries were tagged with unique adapter-indexes. Final libraries were validated on the Agilent 2100 Bioanalyzer, quantified via qPCR (KAPA Biosystems Illumina Library Quantification Kit) and pooled at equimolar ratios