GSM3553529: IC:PBS6_2; Mus musculus; RNA-Seq
For cell culture experiments, at the indicated times after stimulation, media was removed from the wells and cells were washed twice with PBS. RLT (Qiagen) was added to lyse cells and RNA extraction was performed using Qiagen RNAeasy kits as per manufacturer's instructions. For RNA Sequencing library preparation, on-column DNAse digestion was performed as per manufacturer's instructions. Sequencing libraries were constructed following Illumina Tru-seq stranded mRNA protocol.  Paired-end sequencing performed with Next-seq 500 with 76 bp reads from each end.