GSM3319587: rdr2-RPS; Physcomitrella patens; Bisulfite-Seq
Genomic DNA was isolated from protonema, fragmented by sonication, end repaired, and ligated to custom synthesized methylated adaptors. Adaptor-ligated libraries were subjected to bisulfite conversion treatment using the Methylation-Gold kit (ZYMO) as outlined in the manufacturer's instructions. The bisulfite-converted libraries were then amplified by PCR and gel purified (~350-400 bp size range) prior to quantification with a Bioanalyzer (Agilent). DNA was extracted from approximately 500 mg tissue using NucleoSpin® Plant II Midi kit for Genomic DNA extraction from plant and fungi (Macherey-Nagel), according to recommended protocol