454 sequencing of community genomic DNA from Richmond Mine C75 location biofilm June 2006
Libraries were prepared at the University of Illinois, Urbana-Champaign according to the Keck Center's standard protocols, involving shearing of DNA to fragments of 700-1100bp in length, ligation of adaptors for downstream amplification. Barcodes (MIDs) were added during library prep, and the MID-tagged samples were pooled for simultaneous amplification and sequencing.