Vibrio cholerae wt; stationary phase sample; used for normalizations.
This sample was intended for normalization in Marker Frequency Analysis (MFA). Cells were grown overnight in LB (+ glucose) at 30°C under agitation. Genomic DNA was prepared from stationary phase cells. Sequencing libraries were prepared for sequencing by automat, targeting an insert size of 200 base pairs bp. Libraries were sequenced using an Ion Proton sequencer PI chip. MFA was performed essentially as described in Skovgaard et al. (2011) PMID: 21555365; doi: 10.1101/gr.117416.110.