Mixed Microbial Reference Sample
1. Quantify Samples using Qubit. 2. Prepare 0.2 ng/uL dilutions of each 3. Follow Nextera XT protocol a. Tagmentation reaction following recommended procedure b. PCR indexing (N701 and N502, N503) c. PCR cleanup i. Two washes of Buffer PE ii. Two elutions of Buffer EB (20 uL each) d. Quantify using Qubit 4. Library Normalization a. Follow recommendations b. Qubit 5. Library pooling a. Use 12 uL of each normalized sample (approximately 13.4 pmol/L) b. Assume 500 bases as average size (did not measure) 6. Sequence using MiSeq