GSM4187173: Tim-3 deficient Treg-2; Mus musculus; RNA-Seq
Treg cells were sorted from tumors directly into lysis buffer containing 96-well plate libraries were generated using the Nextera XT DNA Library Preparation kit (Illumina).Libraries were normalized, diluted and heat-denatured and then loaded for sequencing. Cluster generation and 75bp paired-end dual-indexed sequencing was performed using the NextSeq® 500/550 High Output Kit v2 on the NextSeq 500 Sequencing System (Illumina)