whole genome sequencing
DNA was fragmented with the Covaris E-210 ultrasonicator. DNA fragments were set to be concentrated in 800-bp peaks for the relevant libraries. These fragments were purified, and we blunted the ends, added A tails and ligated them with adaptors. After size selection in a gel, 10 to 12 cycles of PCR were performed. The concentrations of the libraries were quantified by Bioanalyzer (Agilent Technologies) and quantitative PCR methods, using the ABI StepOne Plus Real-Time PCR system (Life Technologies). Paired-end 100-bp read length sequencing was performed on the HiSeq 2000 sequencer according to the manufacturer's instructions (Illumina).