Genome sequencing of Elysia chlorotica: Illumina 10000bp insert
Genomic DNA was extracted from a pool of ~1000 larvae (whole organisms). Five mate-paired libraries with long-insert sizes (2.5 kb x 2, 5 kb x 2 , 10 kb x 1) were constructed according to the standard protocol provided by Illumina (San Diego, CA, USA). Paired-end sequencing was performed for the five libraries on the HiSeq 2000 platform according to the manufacturers instructions.