Illumina HiSeq 2000 paired end sequencing; RNA-sequencing of murine T-cell acute lymphoblastic leukemia (T-ALL) cells to investigate expression profiling of lymphocyte deficient for Tet2 and mutated for DNMT3A
RNA-sequencing of murine T-cell acute lymphoblastic leukemia (T-ALL) cells to investigate expression profiling of lymphocyte deficient for Tet2 and mutated for DNMT3A
Cells were sorted using flow cytometry based on the expression of GFP and CD4/CD8 markers Allprep Kit (QIAGEN) according to manufacturer's protocol SureSelect Automated Strand Specific RNA library Preparation according to manufacturer's protocol