ITS amplicons of ectomycorrizal fungal root tips: Spatial heterogeneity experiment, control
Ectomycorrhizal root tips were harvested under dissecting scope from pine seedlings grown under each treatment combination. DNA was extracted using a modified Qiagen DNAeasy Bloog and Tissue Kit. Samples were PCRed for the ITS1 region with ITS1F and ITS2 primer pair. PCR products were cleaned with AMpure beads, quantified with qubit, and pooled to equimolar concentration before shipping to be sequenced at theGenomics Sequencing Laboratory, University of California, Berkeley, CA, US