GSM4675922: WT sonication input rep. 1; Saccharomyces cerevisiae; ChIP-Seq
Cells were lysed by mechanical disruption in a bead beater. Chromatin was fragmented using either sonication (all H3K79me3 samples) or MNase (all Sir4-myc and Sir3-EBD samples except for a subset fragmented by sonication, noted in the sample title). DNA was purified using Qiagen spin column kit NEB Ultra II library prep kit