Panaque nigrolineatus hindgut metagenome
Total DNA was isolated from the macerated gut contents of Panaque nigrolineatus using a bead beating approach.  Bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP) was performed using eubacterial primers 939F and 1492R.  Tag-encoded FLX amplicon pyrosequencing analyses utilized Roche 454 FLX instrument with Titanium reagents.  Following sequencing, all failed sequence reads, low quality sequence ends and tags and primers were removed and sequences collections depleted of any non-bacterial ribosome sequences and chimeras using B2C2.