GSM2575301: mix-week5-v2_ATAC-seq; Homo sapiens; ATAC-seq
We performed ATAC sequencing on WM989-A6 melanoma cells according to Buenrostro et al. Briefly, we lysed the cells and set up the transposition reaction with the Tn5 Transposes (Illumina Catalog #FC121-1030) at 37℃ for 30 minutes. We cleaned the reaction with a Qiagen MinElute Kit and then amplified the libraries using custom Nextera PCR primers.