GSM3934451: Forebrain E15.5; Mus musculus; RNA-Seq
Mouse embryonic and postnatal brain structures were dissected from the gestational timepoints E12.5 and E15.5 and from postnatal timepoints P0, P3 and P6. Dissections were performed under a Leica stereoscope with a pair of Moria ultra fine forceps (Fine Science Tools), in a PBS solution. The tissue was transferred into ice cold Leibovitz's medium, followed by single cell dissociation with the Papain System Dissociation. The concentration of the single cell suspension was assessed with a Trypan blue count. Approximately 10,000 cells per sample were loaded on the Chromium Single Cell 3' (10X Genomics) system V2 protocol. GEM-RT, DynaBeads cleanup, PCR amplification and SPRIselect beads cleanup were performed using Chromium Single Cell 3' Gel Bead kit. Indexed single cell libraries were generated using the Chromium Single Cell 3' Library kit and the Chromium i7 Multiplex kit