GSM4186919: ActinomycinD-treated mTEChi 0h; Mus musculus; RNA-Seq
Total RNA was extracted by Trizol NNon-directionnal TruSeq V3 RNA Sample Prep Kit on PolyA+ mRNA was used for WT and Aire-KO mTEChi, as well as for mTEChi used as the reference timepoint (ActinoD 0h), or for mTEChi and lo from the same mice. Smarter Ultra Low Input RNA kit (Clontech) combined to the Nextera library preparation kit (Illumina) was used for ActinoD-treated mTEChi