GSM4502444: shFSCN1 1; Homo sapiens; RNA-Seq
The RNA was treated with RQ1 DNase (Promega, Madison, WI, USA) to remove DNA. The quality and quantity of the purified RNA were assessed by measuring the absorbance at 260 nm/280 nm (A260/A280) using Smartspec Plus (BioRad, USA). The integrity of each RNA sample was further verified by 1.5% agarose gel electrophoresis.electrophoresis. A total 1μg RNA for each sample was used for RNA-seq library preparation. PolyA mRNAs were purified and concentrated with oligo(dT)-conjugated magnetic beads (Invitrogen, Carlsbad, CA, USA) before directional RNA-seq library preparation. The frag¬mented RNA wereend repaired and 5' adaptor ligation. Then, reverse transcription was performed with RT primer harboring 3' adaptor sequence and randomized hexamer. The cDNAs were purified, amplified, and stored at -80°C until they were used for sequencing.