GSM3553651: enoxacin_cel (miRNA-seq); Caenorhabditis elegans; miRNA-Seq
Total RNA was extracted using TRIzol reagent following the manufacturer's instructions (Life Technologies) RNA quality was determined by the 2100 Bioanalyzer (Agilent), and all samples had RNA integrity number (RIN) above 9. For each group, 10 µg of total RNA were enriched for small RNA using PureLink miRNA Isolation Kit (Invitrogen), followed by miRNA library preparation using SOLiD Total RNA-Seq Kit, according to manufacturer's recommendations (Life Technologies).