GSM4502472: LK.zrsr2_ko.1; Mus musculus; RNA-Seq
RNA was extracted using RNeasy columns (Qiagen) per manufacturer's instructions. RNA was Poly(A)-selected, and unstranded Illumina libraries were prepared with the standard TruSeq protocol. To select for fragments <400 bp, 0.5× AMPure XP beads were added to the library followed by 1× AMPure XP beads to select for fragments >100 bp. These fragments were then amplified by PCR (15 cycles) and separated by gel electrophoresis (2% agarose).