GSM4188500: EX18_17_10_DILI-T24H-01_39; Homo sapiens; miRNA-Seq
microRNA was extracted using a miRNeasy Serum/Plasma kit [Qiagen, Venlo,Netherlands] following the manufacturer's instructions. Total RNA was extracted from 50 μL of serum diluted in 150 μL nuclease free water. Briefly, RNA was extracted from the serum by lysis reagent [1000 μL] and chloroform [200 μL]. After centrifugation at12,000 x g for 15 min at 4°C up to 600 μL of the aqueous phase was transferred to a newtube with 900 μL absolute ethanol. RNA was purified on an RNeasy minElute spincolumn and eluted in 15 μL RNase-free water and stored at -80°C. Extraction efficiency was monitored by adding 5.6 x 108 copies of synthetic C.elegans miR-39 spike-in controlafter the addition of lysis reagent before the addition of chloroform and phaseseparation. Standard quality control steps were included to determine total RNA quantity and quality using Agilent 2100 Bioanalyzer with Eukaryote Total RNA Pico Kit and High Sensitivity DNA Assay (AgilentTechnologies, Santa Clara, CA). Libraries were prepared using the TruSeq StrandedmRNA Library preparation Kit (Illumina Inc., San Diego, USA).