GSM3690320: DMSO untreated control rep. 3; Homo sapiens; RNA-Seq
RNA isolation and cDNA conversion were conducted using RNeasy Mini (Qiagen) and High Capacity cDNA RT (Applied Biosystems) kits, respectively, following the manufacturers' protocols. RNA was used for cDNA library construction at Novogene using an NEBNext Ultra mRNA Library Prep Kit for Illumina according to the manufacturer's protocol. Briefly, mRNA was enriched using oligo(dT) beads followed by first strand cDNA synthesis using random hexamer primers. Next, double-stranded cDNA library was constructed through a series of terminal repair, poly-adenylation, and sequencing adaptor ligations and finally size selection and PCR enrichment. The resulting 250-350 bp insert libraries were quantified using a Qubit 2.0 fluorometer and quantitative PCR. Size distribution was analyzed using an Agilent 2100 Bioanalyzer, and qualified libraries were sequenced on an Illumina HiSeq 4000 Platform using a paired-end 150 run (2×150 bases). 30 million raw reads were generated from each library.