Human immunodeficiency virus type 1-pol gene
The Illumina Nextera XT library preparation protocol and kit were used to make DNA libraries. For the tagmentation process the concentration of DNA was 0.2 ng/l. Subsequently, libraries were quantified implementing the Quant-iTTMPicoGreen DNA Assay Kit (Invitrogen) according the manufacturer's instructions. The concentration values were normalized into 4nM for the clusters generation in the lane of the sequencing cell.NGS procedure. Illumina MiSeq instrument with MiSeq Reagent Kit v2 Nano were used to sequence the DNA libraries. We performed 32 paired-end (2x150) sequencing runs. Overall, 1.39 Gbases were obtained. Median number of reads per amplicon was 91994 (range 67185 to 156919).