GSM4072713: 15-1420.Tumour; Homo sapiens; Hi-C
Patient tissue sample was pulverized by repeated hammering while frozen in liquid nitrogen to produce tissue powder. For in-situ Hi-C, tissue was crosslinked, lysed and disgested with MboI. The fragments were the labelled with biotin, proximally ligated, sonicated, and purified with AMPure XP beads. Ligated DNA was pulled down with streptavidin T1 beads, followed by manual library preparation. In situ Hi-C library was sequenced with Illumina Hiseq4000 and Novaseq S4 platforms.