GSM1624192: HE6; Homo sapiens; MBD-Seq
DNA was extracted from formalin-fixed paraffin embedded samples. First, paraffin was removed by treatments with xylene and ethanol. Second, protein was digested using Proteinase K treatment, RNA was removed by RNase A treatment, and protein was removed by 6M NaCl precipitation. DNA was then precipitated by ethanol and re-suspended in water. The library was constructed by enriching for methylated DNA fragments using MiGS (MBD-isolated Genome Sequencing, Serre 2010). After enrichment, library was prepared using the Illumina ChIP-seq preparation protocol (2007).