Whole genome sequencing of Spiroplasma citri strain LB 319
Using LD8 medium, S. citri strain LB319 was isolated from citrus showing stubborn symptoms in Tulare County, CA and maintained in culture. For sequencing, S. citri strain LB319 was triple-cloned and cultured in LD8 medium to obtain enough DNA for sequencing. Total genomic DNA was extracted using CTAB method and library was prepared for sequencing using PacBio SMRTbell Express Template Prep Kit. Whole-genome sequencing was performed on the PacBio RS II platform. Reads were filtered and assembled using SMRT Analysis software from PacBio with default settings.