GSM4278489: 256Cell_Cyt_CAGE; Danio rerio; OTHER
RNA was extracted using the Rneasy mini kit The conventional method used for 5'-cap analysis, also called cap trapping method (Carninci et al., 1996), targets all capped mRNAs by chemical modification of the 5' end (cap) followed by its biotinylation and then a pulldown using streptavidin-coated beads. We used a modified version of cap trapping called Low Quantity CAGE or LQ-CAGE for our CAGE library preparation. This method allowed use of limited quantities of RNAs as is generally obtained in early stages of development.