Hypoxia polysome-bound RNA - replicate 1
For the genome-wide translatomic analysis, RNAs isolated from the fractions 8-11 were pooled to form the polysome-bound samples. The pooled polysome samples from normoxic and hypoxic conditions were used for the preparation of fragment libraries, followed by high-throughput sequencing using a SOLiDTM 5500xl sequencer (Applied Biosystems, Foster City, CA, USA).