Clitoria ternatea root transcriptome
Total RNA was extracted from fresh C. ternatea root using Trizol reagent (Life Technologies). The RNA quality was determined by Agilent 2100 Bioanalyzer. The sequencing and analysis were performed by BGI-Senzhen (China). Briefly, poly(A) mRNA was isolated from total RNA using magnetic beads with oligo(dT) and fragmented in fragmentation buffer. First-strand cDNA was synthesized using random hexamer primers, which is followed by second-strand cDNA synthesis. Short fragments are purified and resolved with EB buffer for end reparation and single nucleotide A (adenine) addition. Suitable fragments were selected for PCR amplification. Finally, the library was sequenced using Illumina HiSeq™ 2000.