GSM3389511: Mouse hypothalamus WT2 (Drop-Seq); Mus musculus; RNA-Seq
Single-cell suspensions were processed through Drop-Seq to generate single-cell cDNA libraries attached to microbeads. Microbeads were counted, and amplified by PCR, and the 3' end of the cDNA prepared for sequencing using a modified Nextera XT protocol. Mouse hypothalamus was dissected and dissociated into single-cell suspension, followed by drop-seq library preparation. Libraries were prepared following the latest drop-seq protcol.