RNA-seq of Macaca mulatta: adult male ileum
Total RNA was isolated from duodenal, jejunal, ileal, and colonic biopsies using the Qiagen mRNeasy kit (Qiagen, Valencia, CA). Ribosomal RNA (rRNA) was depleted using the Epicentre Ribo-Zero rRNA Removal kit (Illumina, Madison, WI). Libraries were constructed using the Bioo Scientific NEXTflex Rapid Directional RNA-seq kit (Bioo Scientific, Austin, TX).