"Depth- and Nutrient-Dependent Variation in Sediment Microbial Community Structure in Loch Leven, Scotland, sample 1S_Arch"
"Sediment samples were collected on 17th September, 2008 from six sample sites within Loch Leven in Scotland (latitude 56o10'N, longitude 3o30'W), selected to represent a gradient of depth (2, 2.5, 3.5, 5.5, 10, 22 m overlying water depth) and other environmental parameters. Sediment cores were taken from a boat using a Jenkin's sediment sampler, and sediment sub-samples were then taken from the cores using sterile 50 ml Falcon tubes. Sediments were homogenized by vortexing. Community DNA was then extracted from 1 g sediment using an UltraClean Soil DNA Isolation Kit (MoBio, Carlsbad, CA, USA). The V3 regions of bacterial and archaeal 16S rRNA genes, and the V8 region of eukaryal 18S rRNA genes, were amplified using universal primers in a two-round nested PCR procedure. PCR amplicons were pooled prior to sequencing on a Roche 454 (Titanium) sequencer. Data is de-noised with the primers trimmed."