DNA seq soil microbiome57bacteriasoil_compost 1fertilizednot inoculatedno trichoyes plantyes soil3
The 16S rRNA gene V4 variable region (for bacteria communities) and ITS1 gene (for fungal communities) were analyzed. Polymerase chain reaction (PCR) primers 515/806 and ITS1F-Bt1/ITS2R, for bacterial and fungal communities respectively, were used in a single-step 30 cycle PCR using the HotStarTaq Plus Master Mix Kit (Qiagen, USA). The PCR conditions were: 94C for 3 minutes, followed by 30 cycles (5 cycle used on PCR products) of 94C for 30 seconds, 53C for 40 seconds and 72C for 1 minute, after which a final elongation step at 72C for 5 minutes was performed. Sequencing was performed at MR DNA (www.mrdnalab.com, Shallowater, TX, USA) on an Ion Torrent (PGM) following the manufacturers guidelines.