GSM2492514: BD015; Homo sapiens; RNA-Seq
CD3+CD4+ and CD3+CD8+ TEM (CD45RA-CCR7-) cells were sorted into CD69+ and CD69- subsets based on the gating strategy in Fig. S1, from spleen and lung tissue of three individual donors (D226, D233, D250, see Table S4), and CD4+ and CD8+ TEM cells were sorted from peripheral blood using the BD-influx sorter. RNA was isolated from cell pellets using the RNeasy Mini Kit (Qiagen), and RNA concentration and quality was assessed using an Agilent 2100 Bioanalyzer instrument (Agilent Technologies). For the majority of samples, >400ng of total RNA was submitted for sequencing standard Truseq with poly-A pulldown