Whole Genome Sequencing analysis of derived from human mid frontal cortex cells
Genomic DNA was fragmented using a Covaris-E210 for 0.75 min. Purified DNA fragments were end-repaired. After A-tailing, the DNA fragments were ligated with paired-end adapters. Adapter-attached DNA fragments of an average 545 bp were gel-purified. The purified DNA fragments were utilized as templates for PCR enrichment (5-6 cycles). The PCR amplified DNA fragments were purified and the enriched library was quantified using a Qubit fluorometer and Quant-iT dsDNA HS Assay Kit (Invitrogen). Library sequencing was performed using 101 BP PE Illumina