GSM1524876: BM2; Homo sapiens; RNA-Seq
RNA of freshly sorted positive cells was extracted and purified using RNAeasy minikit (Qiagen, Maryland, USA). The RNA yield and integrity were determined using BioAnalyzer RNA 6000 Nano (Agilent Technologies, CA, USA). The RNA integrity (RIN) scores were at least 9 in all samples. Libraries were processed with TruSeq RNA Sample Preparation v2 reagents (Illumina, CA, USA) and were quality assessed using electrophoreis (DNA 1K Screen Tape on the TapeStation system, Agilent). Sequencing was performed in a single lane on an Illumina HiSeq-2000 platform with TruSeq Single Reads Cluster Kit v3 and TruSeq Sequencing By Synthesis v3 reagents (by the Australian Genome Research Facility (AGRF), Victoria, Australia).