GSM4676419: DIE_KO_8.5h 8; Homo sapiens; RNA-Seq
Trizol samples were collected of DIE cells that had either an intact DUX4 transgene, or a CRISPR/Cas9 edited DUX4 transgene (DIE_KO), and which were either not induced (0h), 4.5h induced, or 8.5h induced by doxycline. Total RNA samples were subsequently extracted by the addition of chloroform, and isopropanol precipitation The library prep was performed using CEL-seq1 primers, and were processed using CEL-seq2 protocol.