GSM3690436: NBplusNog_510min_038964_0023686; Homo sapiens; RNA-Seq
Cells grown in 6-well or 12-well plates were washed with PBS and lysed with RLT Plus lysis buffer (Qiagen), stored in -20C for 24 hours and then -80C until later processing. Total RNA was purified from the RLT Plus lysates using the RNeasy Plus Mini Kits (Qiagen) according to manufacturer's instructions. RNA libraries were prepared for sequencing using standard the LM-seq protocol.