GSM3933484: Lnc GMR WT; Mus musculus; RNA-Seq
Total RNA was isolated using RNeasy mini kit (Qiagen, Germany). Total RNA was quantified using Bioanalyzer 4200 (Agilent, santa Clara, CA, USA), and RNA integrity was determined using an Agilent 2100 system and an RNA 6000 Nano kit (Agilent Technologies, Santa Clara, CA, USA). The next-generation libraries were prepared using VAHTS mRNA-Seq v2 Library Prep Kit for Illumina® (Vazyme). The Library quality was determined by Bioanalyzer 4200 (Agilent).Then the RNA-Seqlibraries was sequenced in HiSeq X10 system (Illumina) on a 150bp paired-end run.