Illumina HiSeq 2000 paired end sequencing; RNAseq of Mercurialis annua control and selected lines after four generations of selection for increased male allocation in females
RNAseq of Mercurialis annua control and selected lines after four generations of selection for increased male allocation in females
Collect of mature leafs from ten individuals randomly taken from a particular experimental line (diploids) or natural population (tetraploids).  Plant growth in soil with 2 g of slow-release fertilizer per litre of soil in controlled greenhouse conditions Control lines were let to evolve during four generations in populations with 1:1 sex ratio. Selected lines are one females let to evolve in male-deprived population, reproducing only through inconstancy of sex expression Pools of tissues from 10 individuals. Total RNA extraction using  Maxwell 16  Instrument IVD processor Nucleic acid and protein purification (Promega) following manufacturer recommandations Illumina stranded TruSeq RNA library incl. poly(A) enrichment