NextSeq 500 paired end sequencing; Immunologic pathways in protective versus maladaptive host responses to attenuated and pathogenic strains of Mycoplasma gallisepticum
Immunologic pathways in protective versus maladaptive host responses to attenuated and pathogenic strains of Mycoplasma gallisepticum
Trizol reagent was pipetted over the tracheal lumen epithelium four times  Trachea from euthanized chickens were isolated ex vivo  Chickens were exposed to bacteria or medium control via 200 microliter intra-tracheal inoculation Illumina RTA v2 was used for base-calling Total RNA was collected in TRIzol, extracted using the Zymo direct-zol mini prep, and enriched using Illumina PolyA selection per product instructions RNA libraries were prepared for sequencing using standard Illumina protocols (TruSeq Stranded mRNA library prep kit HT)