GSM2492174: Rpo21-HTP_K1246R8; Saccharomyces cerevisiae; OTHER
Extracts from UV-crosslinked yeast cells were used to isolate RNAs cross-linked to a tagged protein in a 2-step purification protocol under denaturing conditions. When applicable, additional purification steps are indicated in the SAMPLES section. RNAs were partially digested. Sequencing 3' adapter and 5' adapter were ligated while protein-RNA complexes were bound to Ni-NTA agarose. RNA library was reverse transcribed and PCR amplified.