GSM2492146: tdT negative replicate 4; Mus musculus; RNA-Seq
Cells were lysed in TRI-reagent (Zymo Research) and RNA was subsequently isolated using the DirectZol RNA Microprep Kit (Zymo Research) according to manufacturers instructions. Library preparation was performed with 220ng total RNA using the TruSeq Stranded mRNA LT Sample Prep Kit (Cat# RS-122-2101, Illumina, San Diego, CA, USA). Libraries were quality-checked on the Fragment Analyzer (Advanced Analytical, Ames, IA, USA) using the Standard Sensitivity NGS Fragment Analysis Kit (Cat# DNF-473, Advanced Analytical). Samples were pooled to equal molarity. Each pool was quantified by PicoGreen Fluorometric measurement in order to be adjusted to 1.5pM and used for clustering on the NextSeq 500 instrument (Illumina).