GSM3933552: microglia, TREM2 -/-, young IL10_M; Mus musculus; RNA-Seq
Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and astrocytes (ACSA-2+) were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina with the unique-molecular identifier add-on kit (Lexogen), following the 'low-input' procedure defined by the manufacturer.