GSM4073042: U2OS cells, B9.G0.R1; Homo sapiens; RNA-Seq
Total RNA was extracted as described above using the NucleoSpin RNA kit (Machery Nagel, Cat. No. 740955) About 500 pg of RNA per sample was reverse-transcribed and amplified using a modified SMARTseq2 protocol (Rambow et al. 2018).  Prior to generating sequencing libraries using the NexteraXT kit (Illumina, Cat. No. FC-131-10)