GSM2332412: asyncC; Homo sapiens; RNA-Seq
The media was removed and the cells were collected with TRIzol Reagent (Ambion). Total RNA was isolated using miRNeasy (Qiagen). Biotin-azide was then tethered to EU with the Click-iT Nascent RNA Capture Kit (Invitrogen). Biotin-EU-RNAs were pulled down from total RNA using streptavidin-coated magnetic beads (Invitrogen). The streptavidin bead-tethered biotin-EU-RNA was used for stranded, ribo-depleted cDNA library generation (Ovation Human FFPE RNA-seq Multiplex System).