GSM2571948: hybrid striped bass pooled liver biological rep1; Morone chrysops x Morone saxatilis; RNA-Seq
Tissue samples were excised, flash-frozen in liquid nitrogen and stored at -80°C until usage. Samples were processed using the Qiagen RNeasy Plus Mini Kit per the manufacturer's recommendation for total RNA isolation. Samples were treated with RNase free DNase I (Qiagen) to remove genomic DNA. Total RNA was standardized to 2 µg each sample. Sequencing libraries were then prepared using the Illumina TruSeq RNA Sample Preparation Kit as per the protocol. Samples were barcoded using Illumina TruSeq Indexed adapters.