GSM3995463: F9 undifferentiated [methylRAD]; Mus musculus; MRE-Seq
Genomic DNA was isolated using a standard phenol:chloroform extraction, followed by ethanol precipitation. DNA from various samples was digested with FspEI overnight at 37°C and subjected to electrophoresis through a 2% agarose gel. 30 base pair fragments were cut out, purified, and adaptors were ligated at 4°C overnight (Wang et al., 2015b).