GSM3861277: SZC RNA Seq  Replicate1; Bos taurus; RNA-Seq
Newly isolated bovine superficial zone chondrocytes and deep zone chondrocytes were cultured (in DMEM/F12 plus 10% FBS) for three days prior to performing RNA-Seq analysis. RNA from superficial zone chondrocytes and deep zone chondrocytes were purified using Trizol reagent (Life Technologies). Total RNA was extracted with TRIzol reagent (Life Technologies) followed by DNase treatment with the TURBO DNA-free™ Kit (Thermo Fisher Scientific, Cat#: AM1907) SMART-Seq v4 Ultra Low Input RNA Kit (Clontech) was used according to the manufacturer's instructions and 75 bp single-end reads were sequenced on an Illumina NextSeq 500 instrument.