GSM3933297: AS216P1; Homo sapiens; RNA-Seq
Total RNA was isolated from samples using RNeasy mini kit (Qiagen). Genomic DNA contaminations were removed from RNA samples with the Ambion® DNA-free kit (Thermo-Fisher Scientific). DNA-free RNA concentration and quality were estimated with NanoVuePlus Spectrophotometer (GE Healthcare) and Agilent 2100 Bioanalyzer (Agilent Technologies), respectively. Libraries for RNA sequencing were generated using TruSeq RNA stranded sample preparation kit v2 (Illumina Inc.) following manufacturer's instructions, using 1 ug of total RNA as input material. Libraries were pooled and sequenced with NextSeq 500 sequencer (Illumina Inc.) generating 75-bp paired-end sequences.