FOXK2 ChIP-seq in FOXK1/2 KO mESCs
ChIP assays were performed according to the standard protocols. Chromatin were crosslinked in 1% formaldehyde (Sigma Aldrich) directly in the dish, and DNA was sonicated to 200 to 400 bp fragments by sonication (Bioruptor, Diagenode). 50 to 1000 ug of chromatin of mESCs were incubated with the various antibodies. ChIP-seq libraries were prepared according to the standard protocols (NEB and Illumina)