GSM4187038: WT biorep2 techrep1; Mus musculus; RNA-Seq
RNA samples were extracted from GFP positive cells, sorted from fresh mouse prostate tissue, using TRIZOL (500 μL, Zymo Research) The library was prepared using SMARTer Ultra Low Input RNA Kit for Sequencing v4 (TaKaRa Clontech) and KAPA Hyper Prep Kiet (KAPA Biosystems) according to manufacturer protocols. The resulting double stranded cDNA was sheared using a Covaris LE220 Plus (Covaris) with a 200 bp peak in the 50μl volume setting. The fragmented cDNA underwent end repair, 3′ end adenylation and ligation with barcoded adapters.The libraries were validated using the Agilent Bioanalzyer DNA High Sensitivity kit (Agilent), and quantified using the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific)