Adineta vaga WT small RNA- Replicate 1
Protein lysates were extracted from homogenized Adineta vaga biomass. Small RNA-protein complexes protein lysates were eluted on small-scale ion-exchange Q columns. Expected piRNA fraction was ligated to adaptors: the 3' ligation was done with T4 RNA ligase 2 truncated tominimize the effects of 2'-O-methyl modification of small RNA populations  and the 5' adapter ligation was done with T4 RNA ligase 1. Final cDNA libraries were constructed with custom oligonucleotides, using the proprietary sequences for small RNA library construction provided by Illumina.