16S of mus musculus: statins and gut microbiota
PCR-amplification of the 16S rRNA gene (forward primer: 341F 5Õ-NNNNNNNNNNTCCTACGGGNGGCWGCAG and reverse primer: 785R 5Õ- NNNNNNNNNNTGACTACHVGGGTATCTAAKCC) were carried out for 30 cycles using the following parameters: 2 min 96¡C predenaturation; 96¡C for 15 s, 50¡C for 30 s, 70¡C for 90 s. About 20 ng amplicon DNA of each sample carrying different barcodes were pooled and purified with one volume of AMPure XP beads (Agencourt) to remove primer dimer and other small mispriming products, followed by an additional purification on MinElute columns (Qiagen). Illumina libraries were constructed using the Ovation Rapid DR Multiplex System 1-96 (NuGEN).