GSM4500391: IAA_rep2; Arabidopsis thaliana; RNA-Seq
Roots were collected in liquid nitrogen. Each replicate contained approximately 100 roots. Total RNA from the roots was extracted using TRIzol® Reagent (Ambion, Cat. Num. 15596-018) and DNaseI (Promega), and total RNA yields were measured using an Invitrogen® Qubit™ fluorometer and Agilent Bioanalyzer 2100 microfluidics (Agilent, Santa Clara, CA). Total RNA samples with an RNA integrity number (RIN) above 7.9 were sent to Beijing Genomics Institute (BGI), where they were ribodepleted, and RNA libraries were prepared according to Illumina TruSeq protocol. The libraries were sequenced for 100 bps in paired-end mode on the Illumina HiSeq2000 platform.