Diff-seq on a PCR-amplified synthetic molecule (consensus sequence for SAMN07550324)
The DNA was derived from amplification of a DNA molecule synthesized by ThermoFisher. The strategy of the library prep employed enriches for polymorphisms present in the sample. The DNA sample is first denatured and reannealed, then subjected to mismatch endonuclease cleavage, followed by ligation of sequencing adaptors and amplification.