GSM1624407: H3K27me3 ChIP in Male Setdb1+/+ X/Y MEFs replicate 3; Mus musculus; ChIP-Seq
For ChIP-seq, nuclei were isolated from formaldehyde crosslinked NSCs and chromatin was fragmented by sonication. Chromatin immunoprecipitation was performed with corresponding antibodies. DNA was extracted from the immunoprecipitated fraction following reverse-crosslinking. Sequencing libraries was generated with Illumina's TruSeq DNA Sample Preparation Kit according to manufacturer's instruction.