GSM2573126: 2181; Rattus norvegicus; Bisulfite-Seq
Rats were anesthetized with isoflurane, decapitated and the brain was rapidly removed. The prelimbic and infralimbic mPFC was collected. The collected tissue was immediately frozen in liquid nitrogen and stored in -80ºC. Genomic DNA was isolated with the DNeasy Blood & Tissue kit (Qiagen, catalog number: 69504). Bisulfite conversion was performed with the EZ DNA Methylation-Direct kit (Zymo Research, catalog number: D5020). Whole-genome bisulfite sequencing (WGBS) libraries were constructed with the Illumina TruSeq DNA Methylation kit (Illumina, catalog number: EGMK91324). Following size selection and amplification, the concentration of each library was quantified using the Qubit dsDNA HS Assay and size distribution was evaluated with the High Sensitivity D1000 Screen Tape in the Tapestation system (Agilent Technologies).