GSM4225059: OW8_CT_HFHC_3_8_weeks; Danio rerio; RNA-Seq
RNA was extracted in TRIzol (Life Technologies) from whole pooled (15 individuals/sample) 13 dpf larvae or single livers isolated from WT (AB) fish and purified using the Qiagen RNeasy Mini Kit. RNA quality was verified on the Aligent Bioanalyzer and DNA contamination was removed with the TURBO DNA-free (Invitrogen) kit. Single-end NextSeq Series High-Output RNA sequencing was performed on poly-A selected coding mRNAs at the Dana Farber Center for Cancer Computational Biology.