GSM3318871: ngn3 islets 1; Mus musculus; RNA-Seq
Mouse islets were isolated and endocrine cells were enriched by flow sorting for Ngn3eGFP low and high (please refer to paper). Cells were > 95% viable so did not enrich with MACs. Cells were encapsulated using the inDrop platform (1CellBio), and RNA was extracted according to the protocol of Klein et al., 2015. CEL-Seq linear amplification of RNA/cDNA followed by index primer-based amplification