GSM3613378: HSCs_PBS_rep1; Mus musculus; RNA-Seq
Total RNA was isolated from HSC using a Direct-zol RNA Kit (Zymo Research) kit with DNase treatment. RNA amplification and cDNA generation was preformed using Ovation RNA-Seq System V2 (NuGEN). Libraries were constructed using Ovation Ultralow V2 DNA-Seq Library Preparation Kit following manufacturer's instructions (NuGEN). Samples were pooled and diluted to 10 nM and sequenced on an Illumina HiSeq 4000 instrument (Illumina) to a depth of ± 20 million single-ended 50 bp reads.