GSM2492035: wt_Ler_chipseq_rep1; Arabidopsis thaliana; ChIP-Seq
ChIP was perfored as described in Schiessl et al. 2014, PNAS 111, 2830-2835, except that tissues were fixed with 2% formaldehyde in PBS 1x supplemented with 1 micromolar Di(N-succinimidyl) glutarate (DSG; Synchem) under vacuum for 20 min. Illumina TruSeq ChIP-Seq libraries were constructed as described in Kaufmann et al, 2009, PLoS Biology 7, e90. ChIP-seq: 50 bp single-end reads sequenced with Illumina HiSeq 2500 (Rapid-Run mode) as described by the manufacturer.