GSM1895847: Volunteer 3 Summer; Staphylococcus aureus; RNA-Seq
RNA was extracted using Qiagen's Rneasy Mini Kit following manufacturer's instructions, the where the samples were treated by beat beating in a solution of RLT Buffer (Qiagen) and 1% of β-mercaptoethanol. Amplification Protocol: Enriched mRNA fractions were amplified using the MessageAMP II-Bacteria RNA amplification according to manufacturer's instructions. Amplified samples were converted to ds-cDNA using SuperScript Double-Stranded cDNA Synthesis Kit and purified using GeneClean Turbo for PCR Kit (MP Biomed). Paired-End DNA Sample Prep Kit (Illumina, San Diego, USA) according to manufacturer's instructions