ITS1 and ITS2 of ITS from bulk soil at base of Juglans nigra
DNA was extracted from bulk soils collected from base of Juglans nigra. Extracted DNA was amplified for the Extracted DNA was amplified for the ITS1 and ITS2 regions using a pool of six ITS3 and two ITS4 PCR primers. Sequencing was performed using the Illumina Miseq platform (2x250) at Oak Ridge National Laboratory (Oak Ridge, TN).