GSM2492432: 3hitNSCs_replicate1 (4C-Seq); Homo sapiens; OTHER
4C-seq libraries prepared according to (Rocha et al., 2016) To generate a 4C library for sequencing, we modified a recent protocol (Rocha et al., 2016) as follows: To capture interactions from the SOX2 promoter, the following primer pairs were used: 5’-TCCACTCTCTTGTTGGGATC-3’, and 5’-CTTCTAGTCGGGACTGTGAG-3’ with DpnII and Csp6I for restriction digests. For the generation of 4C libraries, 10e7 vector NSCs and 3-hit NSCs in duplicates were collected, immediately fixed, and processed accordingly. Illumina adapters were added to the primers before generation of 4C libraries for multiplexed sequencing. 4C libraries were subjected to Agilent Tapestation quality control and quantified with qPCR. Libraries were then sequenced on Illumina HiSeq 2500 sequencers with 50-nucleotide single-end reads.