The Effect of C9ORF72 Levels on Induced Motor Neuron Transcription
Human induced motor neurons were generated from iPSCs using the forced expression of Ascl1, Brn2, Myt1l, Ngn2, Isl1, Lhx3, and Neurod1. Motor neurons were identified using a lentiviral Hb9::RFP reporter and FACS sorted. Total RNA was isolated. Libraries were prepared from total RNA using Clontech SMARTer Stranded RNA-Seq kit, with Clonetech RiboGone ribodepletion performed ahead of cDNA generation. Amounts of input RNA were estimated using the Bioanalyzer and libraries produced according to Clontech’s protocol. Library generation and sequencing were performed at the Norris Cancer Center Sequencing Core at USC.