GSM2575153: High plane of nutrition-C59; Bos taurus; RNA-Seq
Total RNA was extracted from frozen arcuate, anterior pituitary and testes  tissue by homogenisation in Trizol. RNA was extracted using RNeasy Universal plus Kit spin columns. RNA was purified using the RNA Clean & Concentrator. Single-end mRNA-seq libraries were constructed using the Illumina Truseq sample prep kit.