H5N1 within-host diversity in humans and poultry in Cambodia
samples were sequenced directly from original material, without passage. Viral RNA was extracted with QiAmp viral RNA mini kit, amplicons were produced by reverse transcription and PCR. Illumina libraries were generated with the Nextera XT DNA Prep kit, and all libraries were sequenced on an Illumina MiSeq v2 kit (2 x 250).