GSM2574350: srna02_hr0_rep1; Homo sapiens; ncRNA-Seq
Adapted small RNA-seq protocol as in Xu P et al., Methods in Next Generation Sequencing, 2015. Importantly, 3' and 5' adapters have 4 nucleotide degeneracy at the ligating ends to reduce ligation biases. Small RNA and 4sU-incorporated RNA extracted from cells using Trizol. DNA for DRIP-seq obtained by extracting the nuclear fraction and treating with protease, fragmented by in vitro AsiSI digestion plus sonication, then DNA:RNA fragments isolated by S9.6 antibody