small RNA-seq of Arabidopsis thaliana: bulked AMIR-2 over-expressing seedlings
RNA were purified with the  phenol-chloroform method, 20 g of the samples were loaded onto  poly-acrylamide gel, the small RNA fraction was isolated and libraries were  prepared using the Truseq Small RNA Library Preparation Kit (Illumina, San  Diego, CA, USA) and the modified protocol described earlier (Czotter et al.,  2018).