GSM2070065: WT2; Mus musculus; OTHER
Cells were lysed and ZFP36L1-RNA complexes were pulled down with Brf1/2 antibody using protein A Dynabeads.  RNA-protein complexes were run on an SDS-PAGE gel, transfered to nitrocellulose and visualised by radiolabelling a proportion of the immunoprecipitated material.  Correctly sized complexes were cut out of the membrane and used to generate libraries. cDNA libraries from four independent experiments were prepared using the RCLIP primers indicated and sequenced using Illumina’s HiSeq2000 (100 bp single end sequencing).  RCLIP primers used for RNA reverse transcription included a barcode at the 5’end with three known bases and four random nucleotides