Histone  ChIP-Seq of WT (KOS) and ICP4 null (n12) HSV-1 Productive Infection in MRC5  cells
MRC5 cells were mock-infected or  infected with WT (KOS) or ICP4-null (n12) HSV-1 at an MOI of 10 PFU/cell for  two hours and ChIP-Seq for ICP4, Pol II, H3, H3K4me3, H3K27ac, H3K9me3, and  H3K27me3 was performed. Illumina libraries were constructed for the IP and  input material. Illumina sequencing was carried out at Tufts University Core  Facility using HiSeq 2500 to generate 50 bp single-end reads.