GSM4502385: AB2033 VSV Lymph node; Mus musculus; RNA-Seq
Mice were perfused with 5 ml PBS via the left ventricle and spinal cord samples were harvested from individual mice. CNS tissues were cut into small pieces and homogenized through a 100mm mesh filter without tissue digestion. After washing, the cell pellet was resuspended in 40% Percoll and the myelin fraction was separated from mononuclear cells by density centrifugation (2200rpm, 20 min at 14ºC with low acceleration and no brake). Samples were flow sorted either using AriaII, AriaIII or Aria-Fusion (BD Biosciences, BD Diva Software) cell sorter. 3' end mRNA libraries were prepared for sequencing using MARS-seq (Jaitin et al, Science 2014)