GSM4676595: male_Atf3-WT_Naive_0_rep1; Mus musculus; RNA-Seq
Mouse dorsal root ganglia were dissociated into a single nuclei suspension as described in Renthal et al. Nat. Neurosci 2018 and Renthal et al. 2020. Briefly, tissue was dissociated by dounce homogenization and clarified by ultracentrifugation through a Optiprep gradient based on a protocol (Mo et al. Neuron 2015) and modified in Renthal et al. 2020. Nuclei were encapsulated using the inDrops platform, into droplets on ice and lysed in the 3 nL microfluidic droplets using a final concentration of 0.4% w/v Igepal-CA630. Single-nuclei lysates were subject to reverse transcription at 50°C without purification of RNA (as previously described in Klein et al., Cell 2015 and Zilionis et al., Nat. Protoc. 2017). Libraries were prepared as in Zilionis et al., Nat. Protoc. 2017, with modifications summarized in the associated manuscript (Renthal et al. 2020) inDrops libraries were constructued as described in Zilionis et al., Nat. Protoc. 2017; single-nucleus RNA-seq