GSM3615046: SN_SubjectMD5534_f; Homo sapiens; RNA-Seq
Perfused frozen tissues were finely minced and dissociated in an extraction media a based on that of Carter et al. 2009 with 0.1% Triton for 10 minutes. Extracted nuclei were washed in 30 mLs of media based on that of Carter et al. 2009 and pelleted by bucket centrifuge. Nuclei were filtered through a 40 um FlowMi filter before FACS sorting for singlets using a DAPI signal. 10X Chromium Single Cell 3' (V2 or V3) (manufacturer's protocol).  Mouse: V3, human: V2.