GSM2069561: ASK10M475R 2; Saccharomyces cerevisiae; RNA-Seq
Total RNA was extracted using UNIQ-10 Trizol RNA Purification Kit (Sangon Biotech, China) mRNA was isolated and fragmented and cDNA was synthesized using the mRNA fragments as templates. The short fragments are connected with adapters, and suitable fragments are selected for the PCR amplification