PCR Free Whole Genome of COLO829 an immortal cell line derived from a metastasis from a cutaneous melanoma male patient: Sample COLO829_Ill
Library preparation and sequencing. 500ng of each DNA was prepared using the TruSeq DNA PCR-Free Sample Preparation Kit (Illumina®, cat# FC-121-3001) following the manufacturer’s protocol with modified Covaris DNA shearing settings: Duty cycle 10%, Cycles/Burst 200, Time 45 sec. The resulting libraries were sequenced on a HiSeq2500 in rapid mode with read length 101x101. Libraries were clustered on HiSeq flowcells and paired end sequenced on HiSeq2000/2500 instruments for paired 101 reads.