SNP genotyping of maize by RAD seq
The RAD libraries of the segregating populations were prepared for single-end sequencing according to Baird et al. with the following modifications: barcodes were 5 bp long, and were at least two mutational steps apart from each other with regard to the first four bases, followed by the fifth checksum base. Libraries were 96-fold barcoded, each genotype at two barcodes, resulting in 7 libraries.