RNA-seq of Holothuria scabra:  body wall
RNA was extracted from tissue using TRIzol Reagent, as per the manufacturers protocol. Following extraction, RNA was assessed for quality by visualisation on a 1.2% agarose gel, and quantified using a Nanodrop spectrophotometer. Total RNA samples were sent to Novogene for library construction and sequenced (paired-end) using an Illumina HiSeq 2500 sequencing platform.