GSM3319018: Sumai3(SU) Lemma treated with 3ADON at 3 dpi replicate2; Triticum aestivum; RNA-Seq
Total RNA from each sample was isolated using the Plant RNeasy Mini Kit (Qiagen, Mississauga, ON, Canada), according to the manufacturer's instructions. All samples were treated with DNase I on column using the Qiagen RNase-Free DNase Set. The yield and RNA purity were determined spectrophotometrically with a Nanodrop 1100 (Thermo scientific, USA), and the quality of RNA was monitored by determining the RNA Integrity Number (RIN) with an Agilent 2100 bioanalyzer and RNA LabChip (Agilent Technologies, Santa Clara, CA, USA). Samples with a RIN value >= 7 were used to prepare cDNA libraries by using the TruSeq RNA Sample Preparation Kit v2 (Illumina). Libraries were prepared according to Illumina's instructions, using the TruSeq RNA v2 LT kit and sequenced on the Genome Analyzer following the manufacturer's protocols (https://www.illumina.com/documents/products/datasheets/datasheet_hiseq2500.pdf). Paired-end sequencing was conducted on the Illumina HiSeq2500, generating 101-nucleotide reads.