Identification of Genes Relevant to Pesticides and Biology from Global Transcriptome Data of Monochamus alternatus Hope (Coleoptera: Cerambycidae) Larvae
Total RNA was extracted from whole larvae using Invitrogen. The purity, concentration and integrity of the RNA samples were detected using Nanodrop, Qubit 2.0 and Agilent 2100 methods. Messenger RNA was extracted from total RNA. Eukaryotic mRNA was isolated. The mRNA was fragmented randomly. Using the mRNA as a template, the first cDNA chain was synthesized. And a second cDNA chain was synthesized. Finally, the cDNA library was created by means of PCR enrichment.