GSM3996902: HEK293-TKD3; Homo sapiens; RNA-Seq
RNA was harvested using Trizol reagent.NEBNext® rRNA Depletion Kit (New England Biolabs, USA) removed ribosomal RNA (rRNA) from 2 μg of total RNA. The purified rRNA-removed RNA was treated using the EZ RNA methylation Kit (Zymo Research). Then the TruSeq Stranded Total RNA Library Prep Kit (Illumina, USA) was used to construct the sequencing library.t was used with 4 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols