GSM2572314: K4H, UV, 4h/30 mJ/cm2 (time/dose)_ChIP-seq; Homo sapiens; ChIP-Seq
Cells were fixed with 1% (final concentration) freshly prepared formaldehyde at 37°C for 15 min. The fixation was stopped by incubation in 125 mM (final concentration) glycine solution for 5 min at RT. Cells were washed with PBS containing proteinase inhibitor cocktail (1x final concentration), scraped and collected as cell pellets in Eppendorf tubes. ChIP assays and sequencing were performed by Active Motif using the H3K27ac HistonePath™ Kit following standard protocols (Active Motif, Carlsbad, CA)