GSM4189617: P4_2; Mus musculus; RNA-Seq
Mouse E14.5 brain was dissociated and either mCEL-Seq2 protocol for scRNA-seq or population-based FACS-sorting and bulk RNA-seq was performed. scRNA-seq libraries were prepared according to mCEL-Seq2 protocol. Libraries were sequenced on an Illumina HiSeq 3000 sequencing system (pair-end multiplexing run, high output mode) at a depth of ~200,000 reads per cell. Population-based FACS-sorting and bulk RNA-seq  from neural cells was isolated using the Qiagen mini kit (#74104), while RNA from mural cells, endothelial cells and microglia was extracted using the Qiagen miRNeasy Micro kit (#1071023). Libraries for RNA-seq of the neural population were prepared using the Illumina TruSeq library preparation kit. For mural cells, endothelial cells and microglia, cDNA was prepared from isolated RNA using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech 634891). Libraries were prepared using the Nextera XT DNA library preparation kit (Illumina FC-131-1096) and sequenced on the HiSeq 2500 instrument. scRNA-seq