GSM3861382: Gm15441-LSL liver_Control diet_Pool1_G167_M4; Mus musculus; RNA-Seq
RNeasy Plus Mini Kit (Qiagen, Valencia, CA, USA) was used to extract total RNA from livers from four different treatment and control groups: wild-type mice and Gm15441-LSL mice fed either control diet or fed a diet containing 0.1% WY-14643 for 24 hours and euthanized between 1 and 3 PM. RNAs were extracted from individual livers, followed by quality assessment of RNA by TapeStation 4200 (Agilent, Santa Clara, CA, USA), to identify high quality RNA samples (RIN>9.0) for pooling. Stranded RNA-seq libraries were prepared from polyA-selected total liver RNA using an Illumina stranded TruSeq mRNA Prep Kit (Illumina, San Diego, CA, USA). Three independent RNA pools were prepared for each of the 4 treatment groups, with each pool comprised of n = 2-3 individual liver RNA samples. RNA-seq libraries were subjected to 150 cycle paired-end sequencing using an Illumina HiSeq 2500 instrument (Illumina) at a depth of 13-21 million read pairs for each of the 12 RNA-seq libraries.