GSM3932578: LH4 [RNA-Seq]; Sus scrofa; RNA-Seq
Total RNA was isolated individually from the ovaries samples using TRIzol Reagent (Invitrogen), purified with RNeasy Mini Kit (Qiagen). Total RNA of each sample was quantified and qualified by Agilent 2100 Bio-analyzer (Agilent Technologies, Palo Alto, CA, USA), NanoDrop (Thermo Fisher Scientific Inc.) and 1% agrose gel. Each sample with RNA Integrity Number (RIN) value > 7.5 was used for following library preparation. Approximately 3μg RNA of each RNA-seq sample was used to remove ribosomal RNA (rRNA) using Epicentre Ribo-Zero™ rRNA removal Kit (Epicentre, USA). Each sample was amplified by PCR to enrich cDNA libraries.