single cell RNA-seq of human cell lines
Cellular suspensions were loaded on a 10x Genomics Chromium Single Cell instrument to generate single cell GEMs. Single cell RNA-Seq libraries were prepared using GemCode Single Cell 3 Gel Bead and Library Kit. Sequencing libraries were loaded at 10pM on an Illumina HiSeq2500 with 2  50 paired-end kits using the following read length: 26 cycles Read1, 8 cycles i7 Index and 98 cycles Read2.