GSM2069047: Mouse: 4HF27; Strain: A/J; Diet: High Fat, High Sugar (HFHS); Time-point: D30; Mus musculus; RNA-Seq
At least 150 islets were selected and transferred to a 1.5mL DNA/RNA free tube. After centrifugation (1000 rpm, 10s) and disposal of the supernatant, islets were lysed in 350µL RLT Buffer (Qiagen, France). Samples were kept at -80°C before RNA extraction and RNA seq. The Sequencing library preparation was performed using 200 ng of total RNA input with the TrueSeq RNA Sample Prep Kit v2-Set B (RS-122-2002, Illumina Inc, San Diego, CA) producing a 275bp Fragment including adapters in average size.