GSM4223681: STAG3_SMC1B_p3: 2x transgene population 3; Homo sapiens; RNA-Seq
trizol, DNase I, oligo-dT beads enrichment, fragmentation with average size 200nt cDNA libraries were prepared for sequencing using standard protocols, including: first strand synthesis using random hexamer primers, purification of dsDNA with magnetic beads, end repai, addition of single adenine to the 3' termini, sequencing adaptor ligation, and PCR amplification.