GSM1524801: glucose 48 h B replicate; Aspergillus niger; RNA-Seq
Total RNA was extracted from mycelia using the Plant/Fungi total RNA Purification Kit (Norgen Biotek, Canada) including the on-column DNAase treatment step. 10 ug of Total RNA was depleted of ribosomal RNA using the Ribominus Eukaryotic kit (Invitrogen). The KAPA library quantification kit for Applied Biosystems SOLiD platform was used to quantify the libraries by qPCR. SOLiD whole transcriptome libraries were made as outlined in the SOLiD Whole transcriptome kit protocol (Applied Biosystems). Emulsion PCR and bead-based enrichment was carried out using the SOLiD EZ bead system. Using a SOLiD 5500xl ABi sequencer, 50bp/35bp paired-end reads were generated in colour space.