GSM2753799: RNA seq E10.5 WT1Cre #3153 unlabelled; Mus musculus; RNA-Seq
Hepatic stellate cells were isolated from livers by density centrifugation and subpopulations sorted by flow cytometry into RLT with beta-mercaptoethanol. Total RNA was isolated using Qiagen RNeasy columns following the manufacturer's instructions. RNA libraries were prepared for sequencing using standard Illumina protocols