GSM1897503: PRPL_6149; Mus musculus; RNA-Seq
4-week-old mice were sacrificed in accordance with IACUC approved protocol.  Uteri were harvested and digested in Hank’s Balanced Salt Solution (HBSS) containing 0.28 Wünsch units/ml Liberase TM Research Grade (Roche Life Science) and 30 μg/ml DNAase I (Roche), then incubated for 30 min at 37°C with intermittent trituration. Tissues were washed and then resuspended in FACS buffer (PBS, 1% FBS, 5 mM EDTA) and incubated at 37°C for an additional 15 min, after which they were filtered through 70 μm nylon mesh. Samples were then stained with fluorescent-conjugated antibodies to CD45 and CD326 for 20 min on ice.  Samples were washed in FACS buffer and then resuspended in FACS buffer containing 7-AAD. CD45-CD326+7-AAD- (live epithelial) cells were sorted on a BC MoFlo.  RNA was then isolated using a Qiagen RNeasy Mini Kit according to manufacturer's protocol. RNA libraries were prepared for sequencing using standard Illumina protocols