GSM4432416: Organoids scRNA-seq; Homo sapiens; RNA-Seq
Organoid 12 weeks post differentiation dissociated into single cells upon incubation for 1hr with solution containing papain and EDTA. Upon dissociation, we estimated the cell number and viability; then cells resuspended on solution containing 0.5% BSA in PBS to reach concentration of 390 cells per ul. The cDNA  library was prepared using the Chromium Single Cell 3′ Reagent Kits (v3): Single Cell 3′ Library & Gel Bead Kit v3 (PN-1000075), Single Cell B Chip Kit (PN-1000073) and i7 Multiplex Kit (PN-120262) (10x Genomics). RNA libraries were prepared for sequencing according to the manufacturers' instructions (10X Genomics).