Archeal amoA taken from Cape Fear sediment at Site 10 Transplanted to Site 14 Feb-2011
"DNA extraction was performed on estuary sediment samples using PowerSoil DNA isolation kit (MoBio Laboratories, Carlsbad, CA) according to manufacturer instructions. Amplicon pyrosequencing performed at Molecular Research DNA labs (www.mrdnalab.com, MRDNA, Shallowater, TX,USA) using the Archeal amoA primers: Arch-amoAF STAATGGTCTGGCTTAGACG and Arch-amoAR GCGGCCATCCATCTGTATGT with the forward primer being used in the 454 run."