miRNA-seq of I. ricinus
Tick saliva was produced as already described in (Horka H, et al. Tick saliva affects both proliferation and distribution of Borrelia burgdorferi spirochetes in mouse organs and increases transmission of spirochetes to ticks. Int J Med Microbiol. 2009 ). The samples were prepared and sequenced using a 36 bp single-end standard sequencing protocol on a Genome Analyzer II x (Illumina) per the instructions of the manufacturer. The sequencing libraries were prepared according the manufacturerŐs instructions ŇTrueSeq Small RNA Sample Prep GuideÓ. Cluster generation was performed using the Illumina cluster station. The fluorescent images were processed to sequences using the Illumina Genome Analyzer Pipeline Analysis software 1.8