Bacterial amoA taken from Cape Fear sediment at Site 4 Jun-2010
"DNA extraction was performed on estuary sediment samples using PowerSoil DNA isolation kit (MoBio Laboratories, Carlsbad, CA) according to manufacturer instructions. Amplicon pyrosequencing performed at Molecular Research DNA labs (www.mrdnalab.com, MRDNA, Shallowater, TX,USA) using the Bacterial amoA primers: amoA1F and amoA2R GGGGTTTCTACTGGTGGT and CCCCTCKGSAAAGCCTTCTTC with the forward primer being used in the 454 run."