GSM1523061: NCAPG-E2_repeat2 (v.s. E2-ICI)_ChIPSeq; Homo sapiens; ChIP-Seq
Lysates were crosslinked with 1% of formaldehyde or double-crosslinked with DSG first followed by 1% of formaldehyde, clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. The extracted ChIP DNA was ligated to specific adaptors of Truseq system from Illumina, according to the manufacturer’s instructions.