GSM2674562: BN3Toxo; Rattus norvegicus; RNA-Seq
RNA was extracted with RNeazy mini kit (Qiagen) including DNase I treatment following the manufacturer’s instructions. Quality control was performed with Agilent 2100 Bioanalyzer (Agilent Technologies) RNA sequencing libraries were prepared with Illumina's 'TruSeq Stranded mRNAseq Sample Prep kit' (Illumina) following the manufacturer’s instructions. The libraries were quantitated by fluorometry (Qubit), run on a Bioanalyzer for fragment analysis and diluted to 10 nM. The libraries were quantitated by qPCR and sequenced on two lanes for 101 cycles from one end of the fragments on a HiSeq2500  using a HiSeq SBS sequencing kit version 4. Fastq files were generated and demultiplexed with with the bcl2fastq v2.17.1.14 Conversion.