GSM3615239: miR-Ctrl-transfected mouse articular chondrocytes, rep 4; Mus musculus; RNA-Seq
Total RNA was extracted using the TRI reagent (Molecular Research enter, Inc.). One microgram of total RNA (RIN > 9.5) was used to obtain cDNA libraries using Truseq Stranded mRNA LT Sample Prep kit (Illumina). Final cDNA libraries were analyzed for size distribution, quantified by qRT-PCR (Kapa Biosystems) using Agilent 2100 Bioanalyzer, and normalized to 2 nM for sequencing.