GSM4432201: IL33_MHCII+_3_ATAC; Mus musculus; ATAC-seq
ATAC-seq libraries were generated as previously described with minor modifications (Buenrostro et al., 2013). In brief, approximately 50,000 sorted cells were washed and lysed. Then, nuclei were tagmented by incubation with Tn5 for 30 min at 37°C. Tagmented genomic DNA was then amplified by PCR for 15 cycles. The libraries were size-selected (100–600 bp) by Ampure XP beads (Beckman Coulter). The concentrations of the final libraries were quantified by Qubit (Thermo Fisher), and fragment lengths were quantified by Fragment Analyzer (Advanced Analytical). The pooled libraries were paired-end sequenced on a NextSeq 500.