GSM4431903: Human infant foreskin epidermis - Donor 3; Homo sapiens; RNA-Seq
Human neonatal foreskin epidermis (n=5). Samples were surgically excised and placed in HBSS + 10% FBS at 4C until retrieved from the hospital that collected the tissue samples. The tissue was placed in dispase at 37C for several hours until the epidermis could be peeled apart from the dermis. The epidermis was then trypsinized for 30 min, and the dermis was discarded. Epidermal cells were incubated with DNAse for 5 min, filtered over 40um cell strainer, and flow sorted for live cells for library preparation. Single cells were captured using the Chromium Platform (10X Genomics) and libraries were generated using Single Cell 3' v2 chemistry. Library metrics were as follow: 550.19 pg/µl with an avg. size ~454 bps. Libraries were sequenced on an Illumina Next-Seq4000 platform (Illumina). Cell counting, capture, library preparation, quality control, and sequencing was performed at the Genomics High Throughput Sequencing facility at the University of California, Irvine. Single Cell 3' v2 chemistry - 10X Genomics