Diff-seq on a 1:4 mix of 2.7 kb (pol gene) HIV clones
The DNA was derived from amplification of HIV clones. The strategy of the library prep employed enriches for polymorphisms present in the sample. The DNA sample is first denatured and reannealed, then subjected to mismatch endonuclease cleavage, followed by ligation of sequencing adaptors and amplification.