RNAseq of Trypanosoma congolense: tsetse mouthparts
Total RNA from dissected hypopharinxes were extracted with the AllPrep™ RNA/Protein Kit (Qiagen, UK) according to the manufacturer’s protocol, yielding RNA outputs of 48 to 213 ng per sample. RNASeq libraries were prepared at Centre of Genomic Research (Liverpool, UK) using the NEBNext® Ultra™ II Directional RNA Library Prep Kit with polyA selection (Poly(A) mRNA Magnetic Isolation Module) (New England Biolabs, UK) as per standard procedure. RNASeq libraries were sequenced on the HiSeq2500 platform (Illumina Inc, USA) as 150 paired ends, producing 280 million mappable reads.