GSM1895358: bsd-VEC#2; Homo sapiens; RNA-Seq
Qiagen Rneasy minicolumn Total RNA was prepared using Qiagen RNeasy mini kit, processed using TruSeq RNA sample preparation kit (Illumina) to generate libraries of sequencing molecules. samples were prepared from four biological replicates of viral infections, indexed, pooled, and sequenced. using Illumina HiSeq2500 instrument. Both vector and WWTR1 were prepared with four biological replicates by lentivirus infection (bsd-, puro-, #1 and #2). Twelve indexed samples (including four from other projects) were pooled and sequenced in two HiSeq channels. Raw data were demultiplexed and pooled from two channels for differential expression analysis.