GSM1622679: hCASMC_WT1; Homo sapiens; Bisulfite-Seq
genomic DNA was extracted using the Qiagen AllPrep DNA/RNA Micro kit, and was then bisulfite converted with the Qiagen Epitect Bisulfite Kit. Libraries were prepared following manufacturer's instructions using the Ovation® SP+ Ultralow DR Multiplex System (NuGEN Technologies Inc.) and were sequenced on an Illumina MiSeq with 150 base pair paired-end reads.  Data was analyzed by the Next Generation Sequencing Core at the University of Pennsylvania (Philadelphia, PA) DNA sequencing libraries were prepared from PCR-amplified bisulfite converted genomic DNA