bovine metagenome
The following two amplification steps were performed: an initial PCR amplification using 16S locus specific PCR primers (16S-341F 5-CCTACGGGNGGCWGCAG-3 and 16S-805R 5-GACTACHVGGGTATCTAATCC-3) and a subsequent amplification integrating relevant flow-cell binding domains and unique indices. Libraries were sequenced in a MiSeq (Illumina, CA) in paired end with 300-bp read length.