GSM4502419: Neuroblastoma cell line (SH-SY5Y) with SWT rep2; Homo sapiens; RNA-Seq
Total RNA was isolated using  RNeasy Mini Kit 50 (Qiagen) according to the manufacturer's protocol. Library preparation was performed with the TruSeq® Stranded mRNA HT technology, according to the manufacturer´s protocol . Quality was checked by DNA 1000 LabChip kits on the 2100 Bioanalyzer (Agilent Technologies) and quantified using the highly sensitive fluorescent dye-based Qubit® ds DNA HS Assay Kit (Invitrogen)