Illumina HiSeq 2500 paired end sequencing; AtPRMT5 regulates shoot regeneration through modulating multiple genes expression analyzed by RNA-seq
AtPRMT5 regulates shoot regeneration through modulating multiple genes expression analyzed by RNA-seq
Three biological repeats of wild type (wild type-A, wild type-B and wild type-C) and the mutant atprmt5-1 (atprmt5-A, atprmt5-B and atprmt5-C) cultured on shoot-induction medium for 16 days were used for RNA sequencing. Total RNAs were isolated from the calli of wild type Col and the mutant atprmt5-1 with TRI reagent (Sigma, catalog number T9424). The RNA-seq llibraries were constructed with TruSeq Stranded mRNA Library Prep Kit (Illumina, catalog number RS-122-2101).