RNA-Seq of wild type mus musculus with mild peripheral nerve injury: young male, isolated muscle stem cell
Briefly, total RNA was isolated from sorted  cells using Trizol (Thermo) and the miRNeasy Micro Kit (Qiagen) as per the  manufacturers instructions and RNA concentration and integrity were measured  with a Nanodrop spectrophotometer (Nanodrop 2000c) and Bioanalyzer (Agilent  2100). Approximately 10ng of isolated total RNA was used to produce cDNA  libraries using the SmartSeq4 protocol (Clontech), as per the manufacturers  instructions. Individual libraries were pooled and sequenced using 76-bp  paired-end reads on an Illumina NextSeq to an average depth of 50M reads per  library.