GSM4676615: IL-6_IL-21_rep3; Mus musculus; RNA-Seq
RNA from in vitro Th cultures was harvested by placing 200,000-1,000,000 Th cells in Trizol reagent. RNA was isolated from these preparations via phenol-cholorofrm extraction. Illumina-sequenced reads from these cells were further quality filtered, aligned to the mouse genome (mm10), postprocessed, quantified and analyzed by Otogenetics.