GSM4186374: FFPE_input; Mus musculus; ChIP-Seq
FFPE block was histologically examined and sectioned. Sample was deparaffinized, rehydrated and homogenized  by heating at 65C in 1% SDS detergent buffer overnight. Next sample was sonicated for 5 minutes and an aliquot of solubilized chromatin (2.5% of the sample) was used to prepare input DNA by reverse cross-link overnight at 65C with proteinase K. Libraries were prepared with Accel-NGS 2S Plus DNA Library Kit (Swift Biosciences) or SMARTer® ThruPLEX® DNA-Seq Kit (Takara Bio USA Inc).