Pristina leidyi regeneration/fission transcriptome sequencing
cDNA was synthesized with a modified oligo-dT primer using a MINT full-length reverse-transcription kit. The cDNA sample was split into two fractions, one of which was normalized and the other of which was left un-normalized, which were then pooled. The cDNA to be normalized was subjected to normalization using a duplex-specific nuclease (DSN) and subsequenctly PCR amplified. Unamplified, non-normalized material was combined with the normalized material in a 1:2 ratio. This pooled cDNA library was used for 454 pyrosequencing.