RNA from vesicles of A. laidlawii PG8R10
Acholeplasma laidlawii was treated ciprofloxacin (0.5x MIC). The isolation of the  A. laidlawii PG8R10 extracellular vesicles was performed on 3 biological  replicates. The vesicles were treated by DNAse and RNAse. RNA was extracted  with the RNeasy Micro kit (Qiagen). To construct library using the NEBNext  Small RNA library prep kit (Illumina). Libraries were sequenced using the  HiSeq 2500 system (Illumina).