Viral cDNA & MLV mutants
HEK293mCAT cells were infected through a transient transfection of plasmids bearing a replication competent MLV virus and other such plasmids bearing mutations in the integrase gene. The purpose of the project was to detect if there was any appreciable changes in the preference MLV has for local DNA sequences at the site of integration. 
Cells were infected with MLV or mutant variants, genomic DNA harvested and integration site distributions analyzed via 454.