16S of Pyropia interalgal microorganisms:  healthy Pyropia
Total genome DNA of the samples was extracted by CTAB method (Doyle 1987). The V3V4 regions of 16S rRNA gene were amplified with the primer pair: 341F (5-CCTAYGGGRBGCASCAG-3) and 806R (5-GGACTACNNGGGTATCTAAT-3). PCR reactions were carried out in 30L reactions with 15L of Phusion High-Fidelity PCR Master Mix (New England Biolabs). The samples with bright main strip between 400-450bp were chosen for sequencing.