GSM4502457: Vehicle rep 2; Mus musculus; RNA-Seq
Nuclei were isolated, washed, and lysed with a 10% NP40 buffer and centrifuged. The pellet was washed 2x with TRIzol™ reagent (Invitrogen) to remove unbound RNA. The chromatin pellet was then dissolved in Trizol at 65°C and RNA was isolated using standard protocols  for Qiashredder and RNeasy column purification). Sequencing libraries were constructed using using the TruSeq stranded total RNA kit (Illumina). Sequencing libraries were constructed using standard Illumina TruSeq Stranded mRNA library prep protocol