GSM4185172: dentate gyrus: Tg1; Mus musculus; RNA-Seq
The DGs from WT and APP/PS1 mice were dissected in ice cold PBS with Pen/Strep and the cell dissociation was done using Neural Tissue Dissociation Kit (P) (Miltenyi Biotech) as described (Bhattarai et al., 2016). Cells were next FACS sorted using Propodium Iodide Cell viability dye. Single cell analyses were performed as described (Cosacak et al., 2019). Sequencing was performed by 10X Chromium according to the manufacturer's protocol (Zheng et al., 2016). 10X Chromium