GSM3243758: Hsp90GFP rep1; Drosophila melanogaster; RNA-Seq
RNA was isolated from 5 whole wandering third instar larvae using Trizol (Invitrogen, USA) reagent as per manufacturer's protocol. 1µg of the isolated RNA was subjected to DNase treatment using 2U of TurboTM DNase (Ambion, Applied Biosystem) enzyme for 30 min at 37°C. The reaction was stopped using 15mM EDTA followed by incubation at 65°C for 5-7 min and purification on RNeasy column (Qiagen). The purified RNA samples were processed for preparations of cDNA libraries using the TruSeq Stranded Total RNA Ribo-Zero H/M/R (Illumina) and sequenced on HiSeq-2500 platform (Illumina) using 50bp pair-end reads, 8 samples per lane and each sample run across 2 lanes. RNA libraries were prepared for sequencing using standard Illumina protocols.