GSM4502849: TWP3; Mus musculus; RNA-Seq
Total RNA was isolated from snap frozen PyMT tumors using the peqGOLD Total RNA Kit (VWR International, Darmstadt, Germany). RNA samples were analyzed on a 2100 Bioanalyzer using Agilent RNA 6000 Nano chip (both from Agilent Technologies, Santa Clara, USA). Library preparation was performed using the SMARTer® Stranded Total RNA Sample Prep Kit –HI (Takara Bio Europe, Saint-Germain-en-Laye, France). Quantity and quality of the cDNA libraries were determined by Qubit™ dsDNA HS Assay Kit (Thermo Fisher Scientific, Dreieich, Germany) and Agilent High Sensitivity DNA chip (Agilent Technologies).