GSM2491722: CO-2 [mRNA]; Oreochromis niloticus; RNA-Seq
About 10 ug total RNA of each liver sample was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). Six cDNA libraries were created by reverse-transcription (RT) based on the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA). Finally, we performed the paired-end sequencing (300 ± 50 bp) on an Illumina HiSeq 2500 at the (LC Sciences, USA) according to standard methods. We aligned reads of CO-1, CO-2, CO-3, HTS-1, HTS-2 and HTS-3 groups to O. niloticus (http://www.ncbi.nlm.nih.gov/genome/?term=Oreochromis%20niloticus) reference genome by using of Tophat package. The methods of alignments and annotation were based on. Following the manufacturer's procedure, total RNA of liver samples was extracted by using of Trizol reagent (Invitrogen, CA, USA).