Brugia malayi Female 120 dpi
Parasites were obtained from FR3 where they were isolated and separated by sex from infected gerbils. Worms were homogenized in Trizol (ThermoFisher). Ribosomal RNA (rRNA) depletion was performed using Terminator (Epicentre). Libraries were prepared using the NEBNext¨Ultra RNA Library Prep Kit for Illumina¨Ę(New England Biolabs). Library quality was assessed using a D1000 ScreenTape Assay (Aligent) prior to sequencing. Library concentrations were assessed using the qPCR library quantification protocol (KAPA biosystems).Ę Libraries were sequenced on the Illumina HiSeq platform with 150bp paired-end reads.