Zea mays L. subsp. mays NC310 (Ames 27151; Schnable Lab Ac4733) shoot apex RNA-Seq
"14-day-old shoot apices were collected individually and ground in liquid nitrogen. All RNA extractions were conducted using the Qiagen RNeasy kit cat# 74904 (50), according to Qiagen's protocol. Total RNA was eluted twice with 30 ul RNase-free water. The indexed RNA-Seq libraries were prepared using the Illumina protocol outlined in ""TruSeq RNA Sample Preparation Guide v2"" (Part# 15026495 Rev. D September 2012). The indexed libraries from six inbred/line were combined and seeded onto one lane of the flowcell on Illumina HiSeq 2000 sequencer. The libraries were sequenced using 110 cycles of chemistry and imaging, resulting in paired-end (PE) sequencing reads with length of 2 x 101 bp"