GSM2328764: rtt105d_BrdU-IP_S-phase-45-HU_rep2; Saccharomyces cerevisiae; ChIP-Seq
DNA prepared above were immobilized on MyOne C1 beads (Life Technology). Primers were added by PCR and adaptors by ligation. Then DNA were eluted as ssDNA at 95°C. The ssDNA were amplified by PCR reaction, and the amount of each sample was verified by qPCR. Verified sample DNA were sequenced by Illumina HiSeq generating about 50bp pair-ended reads.