GSM2486650: naïve library pool; Saccharomyces cerevisiae; OTHER
The plasmid DNA from yeast was extracted using the Zymoprep Yeast Plasmid Miniprep II (Zymo Research) reagents and Qiagen miniprep columns. The BH3 library was amplified with PCR using primers that encoded an MmeI restriction enzyme binding site at 5′ end and a universal Illumina sequencing region on the 3′ end. After purification with the Qiagen PCR purification kit, the PCR products were digested with MmeI for 1 h at 37 °C before being heat inactivated. Each digestion product was then ligated to double-stranded DNA fragments containing Illumina adapters, with the adapter containing a unique barcode.