metatranscriptome analysis plant polymer breakdown paddy soil
For ribo-tag analyses, total RNA was extracted from collected soil and rice straw samples according to the method introduced by Mettel et al. (2010). RNA extracts were purified using the RNA clean and concentrator kit (Zymo Research). Purified RNA extracts were reverse transcribed into cDNA and barcoded applying the NEBNext mRNA Sample Prep Master Mix Set 2 for 454 kit (New England Biolabs) following the manufacturers instructions. The fragment size range of individual libraries was assessed using the FlashGel system (Lonza) equipped with 1.2% DNA FlashGel cassettes. Libraries were quantified by fluorometry.   Used adaptor for barcoding: ==========================  RL11 : paddy soil 14 days  RL12 : rice straw 14 days