GSM2573162: S8; Homo sapiens; RNA-Seq
RNA from frozen skin and oral biopsies or cells was extracted using RNeasy kit (Qiagen) according to the manufacturer’s instructions. For biopsies, four samples of each day were processed for RNA extraction, with matching skin and oral wounds from the same subject for that day. cDNA library preparation for RNA-seq analysis was performed on samples collected from skin and oral sampels Day 1, Day 3 and Day 6,  (n=4 for each day) using the Mondrian SP kit (Illumina, San Diego, CA). Sequencing was performed using the Illumina HiSeq 2000.