GSM3318878: mESC 0.5h chase Rep 2; Mus musculus; RNA-Seq
total RNA extraction and IAA-derivatization Targeted mRNA 3' end cDNA libraries were prepared using a protocol described in the Quantseq Flex kit. For details see associated manuscript. Gene-specific forward primers were as follows (sequence in 5  -3  ): ActB,ENSMUSG00000029580, CACGACGCTCTTCCGATCTNNNNNNCCCACTCCTAAGAGGAGGATG; GusB, ENSMUSG00000025534, CACGACGCTCTTCCGATCTNNNNNNGTGGTCTTTGGGAAGGTGAA; Hspa1b, ENSMUSG00000090877, CACGACGCTCTTCCGATCTNNNNNNGGACTGGATGTTCCTCTCCA; Nat10, ENSMUSG00000027185, CACGACGCTCTTCCGATCTNNNNNNTGAAGGGTGGTGTTTTCTCA; Sox2, ENSMUSG00000074637, CACGACGCTCTTCCGATCTNNNNNNATGGCCAACTTTGGCAGTAG; Tnrc6b, ENSMUSG00000047888, CACGACGCTCTTCCGATCTNNNNNNTTTTAGGGGCATCACTGGAG;