De novo assembly transcriptome in chili pepper (Capsicum frutescens) to identify genes involved in the biosynthesis of capsaicinoids
Total RNA from mixed sample was extracted from different tissues using TRIzol reagents (Invitrogen, USA) according to the manufacturer instruction. Each RNA sample was subjected to DNase digestion (TaKaRa, Dalian, China) to remove any remaining DNA, and equivalence pooled them. At least 20ug of total RNA was sent to the Beijing Genomics Institute (Shenzhen, China) for Illumina sequencing (commercial service). In where, a standard cDNA library was constructed and sequencing analysis via Illumina HiSeq™ 2000 was performed.