Corkwing PacBio
An individual was sequenced using PacBio SMRT sequencing and four replicate Illumina HiSeq 2500 runs. Library was prepared using Pacific Biosciences 20 kb library preparation protocol. Size selection of the final library was performed using BluePippin using 7 kb cut-off. The library was sequenced on Pacific Biosciences RS II instrument using P6-C4 chemistry with 360 minutes movie time. In total, 24 SMRT cells were used for sequencing.