GSM4565287: KO_Dam_control; Mus musculus; OTHER
DamID protocol: Lentivirus encoding the control Dam, or LaminB1-Dam fusion, were used to transduce hepatocytes from WT of Tm7sf2/NET47 KO mice, and genomic DNA extracted and processed for DamID as described in Gatticchi et al. (2019) Methods 157:88-99 (PMID 30445179) Genomic DNA was extracted using Qiagen DNA-Easy kit, following manufacturer's instructions. DamID enrichment protocol: Dam-methylated genomic DNA was enriched using the standard protocol as described by Vogel et al. (2007) Nature Protocols 2:1467-1478. It involves digesting gDNA with DpnI enzyme, which cuts at non-Adenine-methylated GATC motifs. Libraries were prepared by Beijing Genomics Institute (BGI) to Illumina's specifications