16s rRNA genes of human gut microbiota
The extracted fecal DNA was used as the template for amplicon sequencing with barcoded primers (341F: 5-CCTAYGGGRBGCASCAG-3) and 806R: 5-GGACTACNNGGGTATCTAAT-3) flanking the V3 - V4 hypervariable region of the bacterial 16S rRNA gene. Approximately 200 ng DNA/sample was used for library construction.