GSM2068268: 3h_10C; Solanum lycopersicum; RNA-Seq
Leaf tissue was sampled, inmediately frozen on liquid nitrogen and stored at -80C. For total RNA extraction, frozen tissue was homogenized to a fine powder and total RNA was extracted using TRIzol® Reagent (Life Technologies), treated with DNaseI (Roche) and further purified with RNeasy Plant Mini Kit (Quiagen). RNA libraries were prepared for sequencing using standard Illumina protocols