GSM2576761: iPSC1-derived microglia rep.2; Homo sapiens; RNA-Seq
RNA extraction was performed using the RNeasy Plus Mini Kit (Qiagen) with QIAshredder (Qiagen). Cells were enzymatically detached after treatment with Accutase for 5 min and using a cell lifter. After centrifuging, cells were washed with PBS and resuspended in lysis buffer. Samples were then stored at -80oC until processed further according to manufacturer's instructions. RNA was eluted in 30μl RNase free ddH2O. RNA libraries were prepared for sequencing using standard Illumina protocols