Targeted Sequencing of  Control population and  individuals with Melanoma: Sample C127040
A targeted, multiplexed PCR primer panel was designed using the Ion AmpliSeq Designer v4.4.4 (Life Technologies, Carlsbad, CA, USA).  Average amplicon size of the panel was 244 bp.  Sample DNA (30ng) was amplified using this custom AmpliSeq primer panel, and sequencing libraries were prepared following the manufacturer Ion AmpliSeq Library Preparation protocol (Life Technologies), using Ion Xpress Barcode Adapters.  Individual sample libraries were pooled, then templated and sequenced on the Ion Torrent PGM Sequencer using Ion PGM Hi-Q Chef chemistry.