GSM1523083: ERa-E2-siCTL_repeat2_ChIPSeq (v.s. ERa-E2-siNCAPD3/H2); Homo sapiens; ChIP-Seq
Lysates were crosslinked with 1% of formaldehyde or double-crosslinked with DSG first followed by 1% of formaldehyde, clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. The extracted ChIP DNA was ligated to specific adaptors of Truseq system from Illumina, according to the manufacturer’s instructions.