WGS of Stylosanthes macrocephala: adult cv. Campo Grande
Sequencing library was generated using NEBNextUltra II DNA Library Prep Kit for Illumina (New England Biolabs, England) and following manufactures recommendations. The genomic DNA was randomly fragmented to a size of 350bp by Bioruptor, then DNA fragments were narrowly size selected with sample purification beads. The selected fragments were then end polished, A-tailed, and ligated with the full-length adapter. After these treatments, these fragments were filtered with beads again. At last, the library was analyzed for size distribution by Agilent2100 Bioanalyzer and quantified using real-time PCR. Libraries were sequenced by Illumina high-throughput NovaSeq 6000 sequencer with paired-end (2150bp) sequencing strategy