GSM3931602: S011_Muscle; Homo sapiens; RNA-Seq
Muscle and adipose tissues were homogenized using TissueRuptor homogenizer. RNA was isolated from both the tissue types using AllPrep DNA-RNA-miRNA Universal Kit. For muscle, library preparation was done in accordance with AmpliSeq Transcriptome Human Gene Expression Kit (A26325, Thermo-Fisher Scientific, MAN00010742 Rev. A.0). 10 ng of RNA was used as the starting material. Libraries for all samples were prepared simultaneously.  Libraries were barcoded using Ion Xpress Barcode 1-16 Kit (4471250, Thermo-Fisher Scientific).  Libraries were quantified using the Ion TaqMan Quantitation Kit (4468802, Thermo-Fisher Scientific) in Fast Mode using the 7900HT (Thermo-Fisher Scientific).  Libraries were diluted to 75pM for sequencing. For adipose, automated library preparation was completed according to the protocol available with the Ion Chef (MAN0013432). 10 ng of RNA was used as the starting material. Libraries were barcoded using the plates available with the Chef Kit. Libraries were diluted to 50pM for sequencing. Template prep and sequencing were performed using the Ion PI Hi-Q Chef Kit with template quantitation using the IonSphere Quality Control Kit