GSM4625129: granulosa cells, T2; Mus musculus; RNA-Seq
Total RNA was extracted from fresh frozen whole ovary samples using Qiagen RNeasy Plus Universal mini kit. The samples were sequenced using a 2x150bp Paired End (PE) configuration. The total number of reads/sample were 45-50 million. Image analysis and base calling were conducted by the HiSeq Control Software (HCS). Raw sequence data (.bcl files) generated from Illumina HiSeq were converted into fastq files and de-multiplexed using Illumina''s bcl2fastq 2.17 software. configuration. The total number of reads/sample were 45-50 million. Image analysis and base calling were conducted by the HiSeq Control Software (HCS). Raw sequence data (.bcl files) generated from Illumina HiSeq wereas converted into fastq files and de-multiplexed using Illumina''s bcl2fastq 2.17 software. RNA sequencing libraries were prepared using 500 ng RNA, and the NEB Next Ultra RNA Library Prep Kit for Illumina using the manufacturer's instructions (NEB, Ipswich, MA, USA)