Altilis A41 DNaseq 600bp selection
Genomic DNA extracted with CTAB method and fragmented using Coveris S3 instrument. Library was prepared following Illumina TruSeq protocol starting from 3ng of DNA. Fragments were selected on agarose gel prior PCR enrichment step to target an average fragment size of 600bp. PCR enrichment was carried out with 5 cycles. This library was constructed with the aim to obtain low coverage sequencing (>5x) for this sample, being parent of a mapping population (F1). Tha main goal was to collect some long range paired reads in order to improve efficiency in haplotype assembly.