GSM2570998: Day 7-Mock 1; Aedes aegypti; miRNA-Seq
Total RNA was extracted from infected and non-infected mosquito samples using mirVANA following the manufacturer's protocol (Life Technologies). The small RNA libraries were from pools at days 2, 7 and 14 post infection generated from both populations using the  New England Biolabs small RNA library protocol (New England Biolabs) at the UTMB's Next Generation Sequencing (NGS) Core (Galveston, TX). The purified cDNA libraries were sequenced using the rapid run platform and single end 50 base sequencing by synthesis on an Illumina Hi-Seq 1500 using the TruSeq SBS kit v3.