GSM4185430: PCF cells, 3d_4; Trypanosoma brucei; RNA-Seq
Total RNA was isolated from T. brucei at different stages (1 × 10e8 cells/replicate) using the miRNeasy Kit (Qiagen) according to the manufacturer's protocol. An additional DNase1 digestion step was performed to ensure that the samples were not contaminated with genomic DNA. NGS library prep was performed with TruSeq Strand-Specific mRNA Library Prep with PolyA Selection following Illumina standard protocol. Libraries were sequenced on an Illumina NextSeq 500. The RNA-Seq measurement yielded on average 11.1 M single reads of 75 nt per sample.