GSM4073580: Shiraz_3; Mus musculus; RNA-Seq
25 days post-infection, mature spermatozoa from control and infected mice was collected using the swim up method. Briefly, left and right cauda epididymis were dissected, placed into 20ul sterile PBS, chopped using a sterile scalpel and left for 5 min at room temperature for the sperm to be released. Fresh sperm samples were homogenised using the QIAzol lysis reagent and immediately processed for total RNA extraction using the miRNeasy Mini Kit (Qiagen, # 217004). 1000 ng of total RNA was used for library prep using NEB's NEBNextÒ Multiplex Small RNA Library Prep Set (New England BioLabs Inc.)