Elucidating the P. pachyrhizi secretome and potential effectors
To identify the P. pachyrhizi in planta secretome at 10 dpi, an Lazer Capturing Microdessection (LCM) approach was used to enrich for fungal mRNA transcripts relative to plant mRNA transcripts by dissecting fungal cells and adjacent mesophyll cells. RNA extracted from LCM samples was used as a template for cDNA synthesis, linear amplification, and Illumina sequencing.