Diff-seq of 1 kb fragments from vector pET17b and derivative pET17b_1
The DNA molecules were derived from amplification of a region derived from vector pET17b or point mutant derivatives. The strategy of the library prep employed enriches for polymorphisms present in the sample. The DNA sample is first denatured and reannealed, then subjected to mismatch endonuclease cleavage, followed by ligation of sequencing adaptors and amplification.