Eriocheir sinensis_male_liver
Total RNA was extracted, the concentration and quality of extracted RNA were checked on Agilent Bioanalyzer 2100 with RNA 6000 Nano Labchips, and genomic DNA contaminated was removed. The mRNA was isolated with magnetic beads and then broken into fragments and reverse transcribed into cDNA and added with adapters. Finally, cDNA libraries were constructed and sequenced on the Illumina HiSeqTM 2500 platform.