PacBio RS sequencing
10 micrograms of pure genomic DNA was fragmented to 20 kb using the Hydroshear DNA shearing device (Digilab, Marlborough, MA) and size-selected to 9–50 kb using a Blue Pippin according to the standard Pacific Biosciences SMRT bell construction protocol. The library was then sequenced on 64 PacBio RSII SMRT cells using the P6–C4 chemistry.
PacBio long read protocol as described in Pendleton et al (2015;PMID 26121404)