GSM2753129: mm_chip_spreb_irf4_brwd1ko-lowdna.2; Mus musculus; ChIP-Seq
Chromatin from flow sorted small pre-B cells (20×10^6 or 2×10^6 for lowdna samples) was used for each ChIP experiment with anti-IRF4 (M-17, Santa Cruz Biotechnogy, sc-6059X, lot#J2015) antibody. DNA libraries were prepared from the sheared chromatin (200–600 bp). Libraries were sequenced on the Illumina Hiseq2500.