Genome Sequence of Staphylococcus  Strain TGG-5 Isolated from goat suffering from clinical Mastitis
Library preparation was performed at Genotypic Technology's Genomics facility following NEXTFlexDNA library protocol outlined in “NEXTFlex DNA sample preparation guide (Cat # 5140-02).In brief, genomic DNA was sheared to generate fragments of approximately 200-500bp in a CovarismicroTube with the E220 system (Covaris, Inc., Woburn, MA, USA). The fragment size distributionwas checked using Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA) with High SensitivityDNA Kit (Agilent Technologies) according to the manufacturer's instructions. The resulting fragmentedDNA was cleaned up using HighPrep beads (MagBio Genomics, Inc, Gaithersburg, Maryland). Thesefragments were subjected to end-repair, A-tailing, and ligation of the Illumina multiplexing adaptorsusing the NEXTFlex DNA Sequencing kit as per the manufacturer's instruction. The resulting ligatedDNA was cleaned up using HighPrep beads (MagBio Genomics, Inc, Gaithersburg, Maryland) andsize selected (300–600bp) on 2% low melting agarose gel and cleaned using MinElute column(QIAGEN, India). These adapter ligated fragments were subjected to 10 rounds of PCR (denaturationat 98 °C for 2 min, cycling (98 °C for 30S, 65 °C for 30S and 72 °C for 1 min) and final extension at 72 °Cfor 5 min) using primers provided in the NEXTFlex DNA Sequencing kit. The PCR products were purified using HighPrep beads. Quantification and size distribution of the prepared libraries wasdetermined using Qubit flourometer and the Agilent High Sensitivity DNA Kit (AgilentTechnologies) according to the manufacturer's instructions