replicate 3 RNAseq of anterior fragment of Nasonia embryo
Embryos were bisected and total RNA collected from the two halves. Nasonia RNA was spiked into RNA collected from Melittobia digitata at a 1:10 ratio.   using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB #E7420) in conjunction with NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) Libraries were validated and quantified using a Agilent 2200 TapeStation system before being pooled and sequenced on an Illumina HiSeq 2500 sequencer with a paired-end protocol.