Altilis A41 DNaseq 300bp selection
Genomic DNA extracted with CTAB method and fragmented using Coveris S3 instrument. Library was prepared following Illumina TruSeq protocol starting from 3ng of DNA. Fragments were selected on agarose gel prior PCR enrichment step to target an average fragment size of 300bp. PCR enrichment was carried out with 5 cycles. This library was constructed with the aim to obtain high coverage sequencing (>25x) for this sample, being parent of a mapping population (F1). Tha main goal was to collect high confidence heterozygous SNP sites along the genome for further genotyping-by-sequencing exercise.