Exome seq of human: adult male B-Lymphocyte
Samples were sheared to ~200bp using the Covaris ME220 Focused-ultrasonicator, followed by end-repair. The size selected fragments will then have adenines added to the 3 ends of the DNA fragment, Illumina sequence adaptors ligated onto the fragments, followed by library amplification, clean-up, and final library QC.  A majority of the steps in this process will be carried out following Agilent SureSelect XT protocol.  Sequencing will be performed on the NextSeq 500 instrument.