GSM4675517: DOX35; Rattus norvegicus; RNA-Seq
RNA was extracted with RNeasy® Fibrous Tissue Mini kit (Qiagen, Manchester, UK) using ~30 mg of snap-frozen heart tissue from the saline and DOX high group of cohort 1 Input material was normalised to equal input of 100 ng prior to library preparation. Polyadenylated transcript enrichment and strand specific library preparation was completed using NEBNext Ultra II mRNA kit (NEB) following manufacturer's instructions.