GSM2492608: Jurkat A Dam control; Homo sapiens; OTHER
Genomic DNA was extracted using Qiagen DNA-Easy kit, following manufacturer's instructions. DamID enrichment protocol: Dam-methylated genomic DNA was enriched using the standard protocol as described by Vogel et al. (2007) Nature Protocols 2:1467-1478. It involves digesting gDNA with DpnI enzyme, which cuts at non-Adenine-methylated GATC motifs. Libraries were prepared by Beijing Genomics Institute (BGI) to Illumina's specifications