GSM4224252: WT AMPA; Mus musculus; RNA-Seq
Single-cell suspensions were converted to barcoded scRNA-seq libraries by using the Chromium Single Cell 3' Library, Gel Bead & Multiplex Kit and Chip Kit (10x Genomics), aiming for an estimated 2.000 cells per library and following the manufacturer's instructions. Samples were processed using kits pertaining to V2 barcoding chemistry of 10X Genomics. Libraries were sequenced on an Illumina NextSeq500, and mapped to the mouse genome (build mm10) using CellRanger (10X Genomics). Gene positions were annotated as per Ensembl build 84.