Illumina HiSeq 2000 paired end sequencing; Transcriptome profiling of human pancreatic cell lineage specification reveals functional signaling pathways and lncRNAs for cell fate determination
Transcriptome profiling of human pancreatic cell lineage specification reveals functional signaling pathways and lncRNAs for cell fate determination
Total RNA was purified from 1,000 sorted cells using ZR RNA microprep kit (Zymo Research, UAS). The cDNA synthesis and amplification was performed with the SMARTer ultra-low input RNA kit (Clontech, US).