Small RNA seq of human breast cancer cell line: MCF-7
Samples of two breast cancer cell lines (MCF-7 and MDA-MB-231) suspended in TRIzol (Invitrogen, Carlsbad, CA) were obtained from colleagues at the Mitchell Cancer Institute and sent to a next-generation sequencing service provider (Otogenetics, Norcross, GA). Sequencing was accomplished using a high throughput Illumina HiSeq2000 machine with a paired-end protocol that generated 100bp reads. Two sequencing runs for each sample were requested: total RNA-seq and small RNA-seq.