GSM3318288: Danio_rerio_ChIPseq_intestine_5dpf_H3K27me3_wt1; Danio rerio; ChIP-Seq
Intestines were crosslinked, homogenized for chromatin extraction, and sonicated to get ~300 bp chromatin fragments. Input DNA was set aside and chromatin fragments were incubated with anti-H3K4me3 (Millipore 04-745, 2 µl) or anti-H3K27me3 (Millipore 07-449, 2 µl) on protein G beads. After elution, standard Illumina (KAPA-HYPERprep) library preparations were made.