Illumina HiSeq 2000 paired end sequencing; CIITA
CIITA
Blood from Leukocyte Cone was processed using a Ficoll separation method [Neron S, Thibault L, Dussault N, Cote G, Ducas E, Pineault N, Roy A. 2007. Characterization of mononuclear cells remaining in the leukoreduction system chambers of apheresis instruments after routine platelet collection: a new source of viable human blood cells. Transfusion 47(6): 1042-1049 ]. Buffy coat was isolated and washed in HBSS buffer. Buffy coat was subjected to purification via positive selection with CD19-coated beads (Miltenyi AUTOMACS) following manufacturers procedures. 20-40 million cells were crosslinked and immunoprecipitated as per procedure described within "manuscript in preparation". Following manufacturers recommended procedures