GSM1625826: Seed Coat (25-50 mg) control mRNA-Seq biorep 1; Glycine max; RNA-Seq
Ribosome protected fragments were purified by the Sephacryl S400 spin column (GE Healthcare Life Sciences) chromatography according to the ribosome profiling kit (Epicentre) manuals and total RNA was extracted from tissues using phenol:chloroform and a lithium chloride precipitation using a modified procedure from McCarty DR (1986). Sequencing was performed by the Keck Center (University of Illinois) using an Illumina HiSeq 2000. Ribosome profiling and RNA sequencing libraries were prepared for sequencing using standard Illumina protocols