Illumina HiSeq 2000 paired end sequencing of SAMD00184515
The library for ddRAD-Seq (Peterson et al. 2012) was created with slight modifications. The protocol used in the present study is exactly the same as that reported in Sakaguchi et al. (2015). In brief, we used the restriction enzymes EcoRI and BglII (New England Biolabs, Ipswich, MA, USA) for restriction digestion during the library preparation. After the PCR amplification of the DNA, the sequencing libraries were purified with AMPure?XP (Beckman Coulter, CA, USA) and size-selected around 380bp under gel electrophoresis using E-Gel? SizeSelectTM (Life Technologies, Carlsbad, CA, USA). After quality measurement using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), the library was sequenced with 100+100bp paird-end reads in a total of three lanes of an Illumina HiSeq2000 (Illumina, San Diego, CA, USA) by Macrogen (Seoul, South Korea).