GSM1525455: WT wild type (miRNA); Oryza sativa; miRNA-Seq
Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. The quantity and purity of total RNAs were quantified by Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number > 7.0. Small RNA libraries were generated using the Illumina Trueseq Small RNA Sample Preparation kits (Illumina, San Diego, USA) according to the manufacturer’s guide. Two degradome libraries were constructed using leaves of WT and phyB following the methods described recently [Ma Z, Coruh C, Axtell MJ: Arabidopsis lyrata small RNAs: transient MIRNA and small interfering RNA loci within the Arabidopsis genus. Plant Cell 2010, 22(4):1090-1103.]