GSM2753027: WT Nicotine; Mus musculus; RNA-Seq
RNA was isolated from the peritoneal macrophages using the RNeasy Micro Kit. Poly(A)-containing mRNA molecules were isolated from total RNA. Illumina TruSeq RNA Sample Prep Kit v2 was used with 500 ng of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols