GSM2575465: H1-hESC; Homo sapiens; RNA-Seq
Total RNA was extracted using RNeasy Plus Mini Kit (QIAGEN). Agilent RNA 6000 Pico Kit (Agilent) was used to assess the RNA quality, and Qubit RNA BR Assay Kit (ThermoFisher Scientific) was used to quantify the extracted RNA. For Illumina sequencing, TruSeq Stranded mRNA Library Prep Kit was used to prepare library. For PacBio SMRT seqeuncing, library was prepared following Iso-Seq protocol (PacBio Inc.). For size selection of PacBio SMRT seqeuncing, the full-length cDNA was fractioned into four contiguous size ranges (0–1kb, 1–2kb, 2–3kb, >3kb) on a Sage ELF (Sage Science) before constructing SMRTbell templates.