Metagenome of the Ca. Chloroheliales L227-5C enrichment culture: initial enrichment
Genomic DNA was randomly sheared by Covaris sonication and was prepared into sequencing libraries using the Nextera DNA Flex Library Prep Kit (Illumina). DNA was sequenced on an Illumina HiSeq 2500 in High Output Run Mode with 2x125 bp paired-end reads. Preparation and sequencing was performed by the The Centre for Applied Genomics (TCAG; The Hospital for Sick Children, Toronto, Canada).