GSM3861601: LS05_relapse_AML; Homo sapiens; RNA-Seq
Total RNA was extracted with AllPrep DNA/RNA Mini Kit (Qiagen), innuPREP DNA/RNA Mini Kit (Analytik Jena), or TRIzol (Thermo Fisher Scientific) followed by RNeasy Mini Kit (Qiagen) from either bone marrow or peripheral blood, according to manufacturers' instructions. Messenger RNA was captured using the NEBNext poly(A) mRNA Magnetic Isolation Module. Libraries were prepared using the NEBNext Ultra Directional RNA Kit according to manufacturer's instruction, and submitted for paired-end 100, 150 or 75 bp sequencing on Illumina HiSeq 2000, 4000 and NextSeq 500 instruments, respectively.