GSM3995472: si-FL12; Homo sapiens; RNA-Seq
RNA was isolated using Dynabeads mRNA Direct Kit (Thermo Fisher Scientific). NGS libraries were prepared using a modified version of Transeq, as described (Jaitin et al., 2014) We used a derivation of the MARS-seq as described (Jaitin et al. Science 2014), developed for single cell MARS-seq to produce 3' exprssion libraries with a minimum of 3 replicates per sample. 3' RNA-seq for digital gene expression quantitation (single end protocol R2 used for 7N barcode + 8N UMI in fastq header)