Identification of functional genes related to quality and resistance in tobacco
To isolate functional genes related to quality and resistance in tobacco, a high yield and quality tobacco cultivar (Nicotiana tabacum cv Cuibiyihao) was used for gene discovery. Samples from treatments of biotic and abiotic stresses including low temperature and drought, hormone treatments (ethylene, abscisic acid, salicylic acid and paclobutracol), and Rolstonia solanacearum inoculation carried out in tobacco, and their corresponding controls were taken for RNA extraction, respectively. Then equally mixed stressed RNAs and mixed control RNAs were used to prepare two cDNA libraries, a stressed cDNA and a control cDNA pools. Then, cDNA libraries were subjected to 454 GS FLX Titanium Pyrosequencing, respectively.