16S rRNA gene amplicon sequencing (V3-V4 region): oral microbiome
DNA was extracted from saliva using the Oragene-DNA kit.  Single amplicons from V3-V4 16sRNA regions were obtained by PCR and used to prepare the amplicon library with the Nextera XT Index kit. Finally the library obtained was sequenced on the MiSeq system using the MiSeq reagent kit v3 600 cycle (2x300 bp).