GSM3860823: RAW26; Mus musculus; miRNA-Seq
Total RNA extraction was performed based on TRIZOL according to the Manufacturer`s standard protocol. Libraries were prepared according to Illumina's instructions accompanying the TruSeq Small RNA Prep kit v2. Size restriction (140-165bp), purification, and quantification of barcoded libraries were performed using the Library quantification kit - Illumina/Universal. For cluster generation up to 10 libraries per were factored using an Illumina cBot.