GSM4224820: SI_Day30C 3; Mus musculus; RNA-Seq
Small and large intestinal tissue was prepared by PBS washes, chopping, DTT digestion (IEL), collagenase/Dnase digestion (LPL). IEL and LPL were pooled, and then CD45+ live (PI-) cells were FACSsorted into RLT. RNA was isolated using a single cell RNA purification kit (Norgen Biotech Corp.) according to manufacturer's instructions. SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech) was used per manufacturer's instructions for amplification of RNA and subsequent cDNA synthesis. All samples proceeded through NexteraXT DNA Library Preparation (Illumina) using NexteraXT Index Kit V1 or V2 Set A (Illumina) following manufacturer's instructions.