RNA-Seq of rattus norvegicus: primary cell  culture from hippocampus after treatment with miRNA
Cells were lysed in RLT puffer and total RNA was extracted using the RNeasy Mini Kit (Qiagen). 30 ng total RNA was converted to cDNA using the Ovation RNA-Seq System V2 (Nugen Technologies) according to manufacturers protocol. This cDNA was used to construct sequencing libraries using Ovation Rapid DR Multiplex System (Nugen Technologies) according to manufacturers protocol.