GSM4189439: ESC1-3; Mus musculus; MNase-Seq
Harvested cells or pronuclei were transferred into lysis buffer and MNase master mix were added to digest the chromatin. The reaction was stopped by EDTA and protease was added to digest proteins. Then fragmented and naked genomic DNA were released. The released genomic DNA were directly subjected to library construction using KAPA Hyper Prep Kit for the Illumina platform, following the manufacturer's instructions.