GSM3319254: WT-1; Arabidopsis thaliana; RNA-Seq
Guard cells were sorted directly into RNA extraction buffer. Total RNA was extracted with the Rneasy Micro Kit (Qiagen). First, cDNA was synthesized using the Ovation RNA-Seq System V2 (NuGEN) and then sequencing libraries were prepared with the Ovation Ultralow Input System V2 (NuGEN).