GSM4188570: CLIP-seq, Input, HA-Ago2 Replicate B; Mus musculus; RIP-Seq
Cells were washed in 1X PBS and then UV crosslinked at 400 mJ/cm2. Immunoprecipitation was performed with anti-HA magnetic beads (Pierce) overnight at 4°C. seCLIP-seq libraries were prepared according to (Van Nostrand et al., 2017) with TruSeq LT adapters. Input and IP samples were amplified with 9 and 18 cycles of PCR, respectively. Quality control on final libraries was assessed with a fragment analyzer and qPCR for colony forming units prior to pooling and loading on an Illumina FlowCell (HiSeq2000, 40 bp SE reads).