GSM4189612: 0628cL; Mus musculus; RNA-Seq
The freshly dissected embryos were immersed into OCT and snapped frozen with liquid nitrogen. Before sectioning, the frozen tissue block was warmed to the temperature of cryotome cryostat (-20°C). Tissue block was then sectioned into thickness of <7 µm and placed in the center of a poly-L-lysine coated glass slide (CatLog no. 63478-AS, electron microscopy sciences). The slide was kept at -80 °C until use. The sequencing library was built with a Nextera XT kit (Illumina) and sequenced using a HiSeq 4000 sequencer using a pair-end 100x100 mode.