sRNA-seq of C. pentagona (cpe-2017) interface with A. thaliana
Total RNA was isolated using Tri-Reagent (Sigma) and used as input to a home-brewed clone of the NEBNext Small RNA Library Prep Set for Illumina. Bar coded Small RNA-seq libraries were made following the cloned manufacturer's instructions, size selected by gel extraction, quality-controlled, and pooled for single-end, 75nt sequencing on an Illumina NextSeq550 instrument using the high-output kit. Libraries were deconvoluted by barcodes.