GSM4563421: Rhes KO 1; Mus musculus; RNA-Seq
Total RNA extracted from 1 male and 1 female pooled striatum per sample. Qiagen miRNAeasy kit (#217004) was used for total RNA extraction and Dnase-treatment done on-column. Libraries were generated using the Illumina TruSeq stranded total RNA kit following manufacturer's protocol. 500ng of total RNA was rRNA-depleted, cDNA generated, end repaired, adapter-ligated and PCR amplified with 9 PCR cycles.