GSM3615444: PCDF1_veh_AR_repl1; Homo sapiens; ChIP-Seq
Chromatin immunoprecipitations were performed as described previously with minor changes (Stelloo et al, 2018, Singh et al 2019). Samples were crosslinked with 2 mM DSG (CovaChem) for 25 min at room temperature followed by 1% formaldehyde for 10 minutes at room temperature and subsequently quenched with glycine. Samples were lysed as described (Schmidt et al., 2009) and sonicated for at least 10 cycles of 30s on, 30s off using a Diagenode Bioruptor Pico. For each ChIP, 5ug of antibody was conjugated with 50ul Protein A magnetic beads. Antibodies used were AR (sc-816, Santa Cruz), H3K27ac (39133, Active Motif). Immunoprecipitated DNA was processed for library preparation (Part# 0801-0303, KAPA biosystems kit). Immunoprecipitated DNA was processed for library preparation (Part# 0801-0303, KAPA biosystems kit).