GSM4188550: Small RNA-seq, Mirg(fl/+) iN Replicate B; Mus musculus; miRNA-Seq
Total RNA was extracted using TRIzol. Small RNA-seq libraries were prepared using QIAseq miRNA Library Kit (Qiagen). Small RNA-seq libraries were prepared from 100 ng DNase-treated RNA using QIAseq miRNA library kit (Qiagen) with 16 cycles of PCR amplification. Quality control of final libraries was assessed with a fragment analyzer and qPCR for colony forming units prior to pooling and loading on an Illumina FlowCell (HiSeq2000, 40 bp SE reads).