Arabidopsis wild-type and oxt6 mutant poly(A) tags
These tags were prepared as described in Wu et al. (Wu X, Liu M, Downie B, Liang C, Ji G, Li QQ, Hunt AG. Genome-wide landscape of polyadenylation in Arabidopsis provides evidence for extensive alternative polyadenylation. Proc Natl Acad Sci U S A. 2011 Jul 26;108(30):12533-8.). Briefly, cDNA was prepared using an anchored oligo-dT primer flanked by a sequence compatible with Ilumina sequencing.  Double-stranded cDNA was digested with one of two restriction enzymes that recognize a four-base sequence, Illumina-compatible adapters were ligated to the digested cDNA, and the resulting tags amplified and submitted for sequencing.      This sample contains two sets of bar-coded tags .  For wild-type tags, the 5'-end (AHQL04_1) tags possessed either a CATG or ACGT sequence at their ends, followed immediately by the cDNA sequence.  The 3'-end (AHQL04_2) tags possess a code consisting of NNNCC, followed by an oligo-dT tract of up to 18 nts.  For oxt6 tags, the 5'-end (AHQL04_1) tags possessed either a CTCATG or CTACGT sequence at their ends, followed immediately by the cDNA sequence.  The 3'-end (AHQL04_2) tags possess a code consisting of NNNAC, followed by an oligo-dT tract of up to 18 nts.