NanoPARE of C. pentagona (cpe-2015) interface
Total RNA was isolated using Tri-Reagent (Sigma) and used as input to the NanoPARE method of library preparation (Schon et al. 2018 - Genome Res.), where exposed 5' ends of transcripts are sequenced (both capped and uncapped). NanoPARE libraries were prepared by amplifying full length transcripts using target-switching oligo (TSO) reverse transcription and tagmented using the Illumina Nextera DNAFlex kit. 5' ends were selected by amplifying from the TSO with barcoded primers, quality-controlled, and pooled for single-end, 75nt sequencing on an Illumina NextSeq550 instrument using the high-output kit. Libraries were deconvoluted by barcodes.