GSM2412258: D87ALI4; Homo sapiens; miRNA-Seq
Total RNA extraction using Trizol reagent 0.5 µg of total RNA0 were ligated, reverse transcribed and amplified (11 cycles) with the reagents from the NEBNext Small RNA Library Prep Set for SOLiD. Amplified libraries were size-selected from 105 nt to 130 nt with the LabChip XT DNA 300 Assay Kit (Caliper Lifesciences). Libraries were then subjected to 4 additional PCR rounds with the primers from the 5500 W Conversion Primers Kit (Life Technologies), converted with the enzyme kit from the 5500 W Conversion Primers Kit (Life Technologies) and quantified with the Bioanalyzer High Sensitivity DNA Kit (Agilent).