GSM4225024: Control D7 replicate 1; Mus musculus; RNA-Seq
For each sample total RNA from pools of 12 embryoids were extracted with Qiagen Rneasy micro kit ( Qiagen#74004) Libraries were constructed using NEB DNA Ultra library construction kit, with standard TruSeq-type adapters. Libraries size and concentration were assessed using Agilent 2,100 Bioanalyzer. Libraries were multiplexed and 75bp single-end were sequenced on illumina Nextseq 500