DSBCapture of DNA breaks in nuclei of NPCs with control shRNA
Detection of DNA breaks was performed as previously described (DOI: 10.1038/nmeth.3960). Briefly, fixed nuclei were subjected to blunting/A-tailing reactions and Illumina adaptor P5 ligation to DNA broken ends. Genomic DNA will be purified and fragmented by sonication, subsequently ligated to Illumina adaptor P7, and the libraries were amplified by PCR for 15 cycles. Prepared libraries were then be subjected to whole-genome bp paired-end sequencing.