GSM3553428: Tead4KD_F1_B2; Mus musculus; RNA-Seq
8 cell embryos were first treated with acidic Tyrode solution to remove the zona pellucida and then transferred into M2 medium. After washed in PBS without Ca2+ and Mg2+, 10 embryos were manually picked into cell lysis buffer with a mouse pipette. Embryo poly-A mRNA were reverse transcribed by SuperScript II and pre-amplified using Smart-seq2 protocol (Picelli et al., 2014), followed by library generation using TruePrep DNA Library Prep Kit V2 for illumina Kit (Vazyme, TD501-503).