Whole Genome Sequencing of untreated pup 1
The Illumina Nextera kit was used for whole genome sequencing. Three untreated related pups were reimplanted to surrogate moms and the number of changes found from the mm10 mouse reference genome were noted. Parents were also sequenced. Variants present in the parents were then eliminated. This was to see if the number of changes from a reference genome when accounting for variations present in the parents were similar to treated pups.