GSM1894192: Splenic adult; Mus musculus; miRNA-Seq
Naive samples were isolated from spleens. Thymic samples were sorted if they were CD4-CD8+. Cells were isolated into Trizol, stored at -80, then RNA was extracted according to the standard Trizol protocol. 450 ng of total RNA was used with the Illumina TruSeq Small RNA Sample Preparation kit. kit. Libraries were sequenced on a Hi-Seq 2000, generating 50 bp reads.