mWGS of stool
Libraries were created using Nextera XT DNA Library Prep Kit with 200pg of DNA and 1/4 tagmentation and PCR reagent volumes. Tagmentation and PCR reactions were carried out according to the manufacturer's instructions. The reaction mixtures were then adjusted to 50L by adding dH2O, and the AMPure (Beckman Coulter) Cleanup was carried out as per the manufacturers instructions.