Metagenome from soil of Coffea arabica
The V4 subregion of 16S was amplified using the primers 515F (5'-GTGYCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACNVGGGTWTCTAAT-3'). The ITS1 region was amplified using the ITS1F (5'-CTTGGTCATTTAGAGGAAGTAA-3') and ITS2 (5'-GCTGCGTTCTTCATCGATGC-3'). The PCR libraries were quantified using Qubit hs-DS-DNA kit (Invitrogen, Carlsbad CA) on a Tecan Infinite F200 Pro plate. The results were used to normalize all the libraries in a equimolar concentration of 2 nM, which were pooled for sequencing. The sequencing was performed using the platform 2x170pb.