GSM2068331: Untreated_195; Mus musculus; RNA-Seq
Once the tumors reached a size of 1 cm3 and 24 hours after their last injection the mice were sacrificed. Tumors and other tissues were isolated. ¼ of the tumor was stored in RNALater at 4 degrees Celsius for RNA isolation. A miRNA isolation kit was used to isolate and purify small and large RNA molecules in one fraction The Ion Total RNA-Seq kit was used to process the samples. Samples were Poly-A selected prior to library preparation. This library preparation included the cDNA synthesis and purification steps with the Ion Total RNA-Seq kit v2 (Life Technologies, UK) according to manufacturer’s instructions. The Ion PI Template OT2 200 Kit v3 and Ion Sequencing 200 kit v3 (both, Life Technologies, UK) were used according to manufactures instructions for sequencing libraries on the PI chip