Illumina HiSeq 2500 paired end sequencing; Transcriptomic responses of two population strains of the kelp Saccharina latissima to temperature and salinity changes
Transcriptomic responses of two population strains of the kelp Saccharina latissima to temperature and salinity changes
Young sporophytes of S. latissima were raised from stock cultures of clonal male and female gametophytes at the Alfred-Wegener-Institute Helmholtz Centre for Polar and Marine Research (AWI, Bremerhaven, Germany; stock culture #3 2013 Roscoff; 3123 and 3124 Spitsbergen) according to the protocol of Heinrich et al. (2012b). Vegetative gametophytes were isolated from spores of one fertile sporophyte collected at Kongsfjorden (79°N, 11°E; Spitsbergen, Norway) and at Roscoff (48° 43′ 39″ N, 3° 59′ 13.2″ W; Brittany, France), respectively (Bartsch 2018) Bartsch I. (2018). Derivation of clonal stock cultures and hybridization of kelps. In Protocols for Macroalgae Research (pp. 61-78): CRC Press Taylor and Francis Group Heinrich S, Valentin K, Frickenhaus S, John U, Wiencke C (2012) Transcriptomic analysis of acclimation to temperature and light stress in Saccharina latissima (Phaeophyceae). PLOS ONE, 7, e44342. Total RNA extraction was conducted using the method described in Heinrich et al. 2012.  Heinrich S, Frickenhaus S, Glöckner G, Valentin K (2012a) A comprehensive cDNA library of light- and temperature-stressed Saccharina latissima (Phaeophyceae). European Journal of Phycology, 47, 83-94. https://www.tandfonline.com/doi/abs/10.1080/09670262.2012.660639 cDNA libraries were prepared with an Illumina TruSeq RNA Library Prep Kit according to the manufacturer protocol.