GSM4431987: WT Hi 3; Mus musculus; RNA-Seq
FACS sorted monocytes were collected on ice, washed in PBS and RNA was isolated using RNeasy micro kit (Qiagen). Libraries were constructed from total RNA with the 'SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian' (Takara/Clontech) according to manufacturer's recommendations, barcoded by dual indexing approach and amplified with 11 cycles of PCR. Fragment length distribution was monitored using 'Bioanalyzer High Sensitivity DNA Assay' (Agilent Technologies) and quantified by 'Qubit® dsDNA HS Assay Kit' (ThermoFisher Scientific).