GSM3933629: Meis_IP_HL; Mus musculus; ChIP-Seq
Around 500 E10.5 (36so) wild-type FLs and 500 E10.5 wild-type HLs were fixed according to standard procedures for ChIP experiments. For the IP, we used ChIP-IT High Sensitivity Columns (Active Motif). Two anti-Meis antibodies were used simultaneously, one recognizing Meis1a and Meis2a,c and the other recognizing all Meis1 isoforms. ChIP-seq libraries were generated using the NEBNext Ultra DNA Library Prep Kit for Illumina and sequenced (single-end mode and length of 50 bp) on a HiSeq 2500 (Illumina)