GSM3613400: Muscle__SFPQ_KO__polyA.rep1; Mus musculus; RNA-Seq
Skeletal muscles were isolated and frozen in liquid nitrogen. Frozen muscle tissues were powdered in liquid nitrogen, lysed by Buffer RLT (QIAGEN), and digested by proteinase K. Total RNA was extracted using an RNeasy mini kit  (QIAGEN) according to the manufacturer's instructions. Ion Total RNA-Seq Kit (Life Technology)