GSM4676410: THP1 + LF82 6h in growth medium replicat 1; Homo sapiens; Escherichia coli LF82; RNA-Seq
Total RNA were extracted from THP1 macrophages infected by WT LF82 at 1 h and 6h P.I. Control experiments were performed with macrophages alone and bacteria alone cultivated for 1 h and 6 h in DMEM medium at 37°C and 5% CO2 without agitation. RNA extraction was performed as described before (Demarre et al, 2019). Experiments were performed from biological duplicate. Human rRNAs were depleted using the Ribo-Zero kit, then strand-specific libraries were constructed using the TruSeq Stranded Total RNA kit (Illumina). Paired-end sequencing (2 x 100 nt) was performed on a HiSeq 2500 sequencer.