GSM4278522: Tregs, Thrombus_3; Mus musculus; RNA-Seq
FACS-sorted CD45+CD4+EGFP[Foxp3]+CD69+ Treg of IVC-ligated DEREG mice were directly collected in RNA extraction buffer (RLT-buffer supplemented with beta-Mercaptoethanol) Quantity of total RNA was assessed by Qubit 2.0 and quality was checked using a RNA 6000 Pico chip on Agilent's bioanalyzer. Full-length cDNA was prepared from 2 ng total RNA (RIN>8) using Takarabio's SmartSeq v4 Kit with a final cDNA amplification of 12 cycles. Barcoded sequencing libraries were prepared from 1ng full-length cDNA using Illumina's Nextera XT kit according to the manual. Quantity of final libaries was assessed by Qubit 2.0 and average size was determined on a Bioanalyzer 8Agilent) using a HS DNA chip.