GSM4278373: salmIR IFM 72h APF rep 1; Drosophila melanogaster; RNA-Seq
Total RNA isolated in Tri-Pure reagent (phenol-chloroform), mRNA selected via 3 passes over Invitrogen oligo-dT magnetic beads, mRNA was fragmented using heat and fragmentation buffer, RT was performed with Invitrogen SSIII first strand synthesis system and second-strand labeled with dUTP. RNA libraries were prepared for sequencing by the IMP sequencing core facility (Vienna, Austria) using standard Illumina protocols.