Bacterial 16S rRNA genes of Macaca mulatta: adult male colon
Total DNA was extracted from duodenal, jejunal, ileal, and colonic biopsies using Mo Bio Laboratories PowerSoil DNA Isolation kit (Mo Bio Laboratories, Inc., Carlsbad, CA) with a 2-minute bead-beating step. PCR and custom primers were used to amplify the V4-V5 region of the 16S rRNA gene.