Enrichment of VH81X heavy chain in the germinal centre B cells, but not in the plasma B cells
spleen germinal center (GC) B and plasma B cells and bone marrow plasma B cells were sorted from a pool of five SLC-/- mice. Spleen GC B and plasma B cells were obtained by: first enrichment sort for CD19+CD93- and CD19low/+CD93hi cells, followed by purity sort for GC B (CD19+CD95+GL7+) and plasma (CD19low/+CD93hi) cells. Bone marrow plasma B cells were obtained by sorting B220-CD138hi cells.RNA was isolated using RNeasy kit (QIAGEN) and cDNA synthesis was performed according to the SuperScript II RT protocol (Invitrogen). Ig γ transcripts were amplified.