smallRNA-Seq of Mus musculus: Pooled (n = 5) cardiac PNPase CLIP
RNA isolated from human atrial appendages and mouse whole heart (as described above) was sequenced through the West Virginia University Genomics Core Facility. RNA was selected for using the NEXTFLEX Small RNA-Seq Kit v3 (Bioo Scientific). Samples were run on the MiSeq in 36bp single end reads achieving ~1 million clustered reads per sample. Adapter trimming through cutadapt and sequence alignment was performed as indicated above. The reference genome used in generating BAM files included fasta formatted DNA from Ensemble release 96 for human (GRCh38) and mouse (GRCm38).