EY-2-40 Sample 2
RNA quality and concentration were determined using Agilent RNA 6000 Nano Kits (Agilent Technologies, Santa Clara, CA) and libraries were generated using Clontech SMARTer Standards Total-RNA Pico Kits (Clontech Laboratories, Inc., Mountain View, CA). Libraries contained inserts of approximately 200 base pairs and averaged quality scores over Q30. Individual TruSeq libraries were pooled into 2 and sequenced across 3 lanes.