GSM2572319: control zebrafish embryos m6A miCLIP rep1; Danio rerio; OTHER
For miCLIP-seq, mRNA was isolate from the trunck region of zebrafish embryos at 28hpf and the RNA integrity was checked using an Agilent Bioanalyzer 2100. 10 ug of mRNA was first fragmented and immunoprecipitated with m6A antibody and thus obtained m6A containing RNA was subjected to cDNA library construction according to previously reported method [Linder et al. 2015; PMID: 26121403]. The libraries were sequenced using HiSeq2000 or HiSeq3000 (Illumina) in single-read or paired-read mode, creating reads with a length of 101 bp