GSM2491737: ATB rep1; Homo sapiens; RNA-Seq
Pooled serum samples were conducted exosome isolation as previously described. Briefly, the cell debris were removed by differential centrifugation at 1000 g for 10min at 4℃, and 16,500 g for 30 min at 4℃, followed by ultrafiltration (0.22μm filter; Millipore, Billicera, MA, USA). Then the exosome pellet was obtained by ultracentrifugation at 120,000 g for 2 hrs) and washed with PBS.Isolated exosomes were immediately used for RNA extraction by phenol-chloroform method. RNA libraries were prepared for sequencing using standard Illumina protocols