GSM2575608: Bcell.H3K27me3.ChIPseq.1; Mus musculus; ChIP-Seq
Chromatin immunoprecipitation (ChIP) assay was performed as previously described (Scharer et al. Cancer Research, 69:709-717). Briefly, 10^7 B cells or 1^6 Plasmablasts were fixed in 1% formaldehyde for 10 minutes, nuclei isolated, and sonicated to an average chromatin fragment size of 200-600 bp. Chromatin was immunoprecipitated with 1 ug anti-H3K27me3 antibody over night at 4 degrees. Antibody-chromatin complexes were captured with Protein A magnetic beads (Invitrogen), cross-links reversed, and DNA purified. ChIP-seq libraries were constructed from total IP DNA using the KAPA HyperPrep Kit