GSM2753004: IAA-7h; Mus musculus; MNase-Seq
Nucleosomal DNA was prepared using EZ nucleosomal DNA prep kit (Zymo research). Nucleosome digestion was performed at varying doses and a maximal amount of 0.5 U Micrococcal Nuclease for 5 min at 25°C. Libraries of nucleosome fragments were prepared and adapted for deep sequencing using the standard Illumina protocol and sequenced them on NextSeq using NextSeq High V2 chemistry and set up as 1x76bp single end run