GSM2574641: RHG1433_hg19_accepted_hits; Homo sapiens; RNA-Seq
Single cells were processed for RNA-seq using Nextera XT kits (Illumina). Cell lysis, reverse transcription (SMARTer Ultra Low RNA kit, Clontech) and PCR amplification (Advantage® 2 kit, Clontech) were performed on the C1™ Auto-prep module. Approximately 0.15 ng of cDNA from each cell was processed for RNA-seq using Nextera XT DNA library preparation kits (Illumina).