GSM3995536: KO-F-FA-3; Mus musculus; ncRNA-Seq
AM cells were isoalated from Broncho alveolar lavage, Total RNA extracted Small RNA-seq libraries were generated by NEXTflex Small RNA Library Prep Kit v3 for Illumina (BioO Scientific, Austin, TX), followed by deep sequencing on an Illumina HiSeq 2500 as per the manufacturer's instructions.  Briefly, 1-2 ng of total RNA was ligated with chemically modified 3'- and 5'- adapters that can specifically bind to mature micro RNAs, followed by reverse transcription and PCR amplification.  Unique index sequence tags were introduced during PCR to enable multiplexed sequencing.  Each library was assessed for the presence of desired micro RNA population and approximate library quantity by Bioanalyzer High Sensitivity DNA Kit (Agilent Technologies).  Pooled libraries were denatured and loaded onto a TruSeq Rapid flow cell on an Illumina HiSeq 2500 and run for 50 cycles using a single-read recipe according to the manufacturer's instructions.