GSM4676153: VosA-WT; Aspergillus nidulans; ChIP-Seq
DNA samples from each strain were extracted using a MAGnify Chromatin Immuno-precipitation System (Invitrogen, USA) according to the manufacturer's protocol with a modification. Two-day-old conidia from the WT strain, strains containing VosA-FLAG, or VelB-FLAG were cross-linked, washed, homogenized with a Mini-Beadbeater 16 (Biospec, USA), sonicated, and separated with centrifugation. The chromatin extracts were incubated with an anti-FLAG antibody-Dynabead complex. Then, samples were eluted from the beads at 55°C using Proteinase K. Enriched DNA was purified using DNA Purification Magnetic Beads. Libraries were prepared using a TruSeq ChIP library preparation kit (Illumina, CA). The libraries of all the replicates were sequenced using an Illumina HiSeq 2500 system.