GSM2412772: Sox4cKO_CD44plus_replicate 2; Mus musculus; RNA-Seq
50,000 cells per sample were sorted into media then pelleted and lysed in Lysis Buffer (Ambion). Total RNA was recovered with RNAqueous Micro Kit (Ambion), following manufacturer instructions. Bioanalyzer (Agilent) and Qubit (Life Tech) were used to determine RNA quality and concentration. SMARTer Stranded Total RNA-seq: Pico Input (Clontech)