XmnI digested monomers from Cercopithecus solatus
XmnI was used to digest Cercopithecus solatus DNA in vitro. 10 µg of Cercopithecus solatus genomic DNA were digested for 4 h 30 min at 37°C with 60 units of XmnI activity (New England Biolabs) in a total volume of 34 µL. The enzyme was inactivated for 20 min at 65°C. The sample was loaded on a 1% agarose gel after addition of 6.8 µL loading buffer (50% glycerol) and electrophoresis was performed in 0.5X Tris-borate-EDTA buffer, at room temperature for 2 h 45 min at 100 V. The gel was briefly stained with ethidium bromide and then imaged by UV transillumination. Band corresponding to alpha satellite monomers (~170 bp) was cut and DNA was extracted from the gel with the Omega Biotek Gel extraction kit and resuspended in 100 µl of elution buffer. About 220 ng were obtained.