RNA Seq of mus musculus:adult female spleen treated with TL1A-Ig/IL-2
RNA was extracted using TRIzol method with chloroform phase separation and isopropanol precipitation then applied to the QIAGEN miRNeasy mini (QIAGEN, Cat. #217004) column for RNA purification. All RNAs were checked on a NANODRP1000 and Agilent bioanalyzer 2100 PICO chip for RNA purity and integrity. The KAPA mRNA HyperPrep Kits (KK8580) with the  IDT for Illumina Dual Index Adapter kit (Caat. #20021454) per the manufacturers protocol. Briefly, mRNA was captured using magnetic oligo-dT beads. Fragmentation was performed using heat and magnesium. 1st strand cDNA synthesis was completed using random priming. Combined 2nd strand synthesis and A-tailing, adapter ligation, library amplification, and KAPA Pure Beads clean-ups were performed for library preparation. The strand marked with dUTP is not amplified, allowing strand-specific sequencing. All final libraries were checked on Agilents bioanalyzer 2100 High Sensitivity DNA Chip. An equal amount of cDNA library from each sample was pooled for sequencing on the Illumina HiSeq 4000 platform.