GSM2576068: RNA-seq replicate 1 from dox- 24h induced SA-tTA-MyoD ESCs; Mus musculus; RNA-Seq
RNA purification was performed from 10cm plates of ~80% confluent murine ES cells from the cell lines described above. Zymo R1054 RNA purification kit was used to purify DNase treated RNA. Sample collection was performed by briefly washing plates in phosphate buffered saline prior to the addition of RNA lysis buffer. Sterile cell lifters were used to disrupt cells, and lysates transferred to microfuge tubes for cold storage prior to purification according to manufacturer-supplied protocol. Purified samples were evaluated by Agilent Bioanalyzer and prepared for RNA sequencing libraries using Illumina reagents. Library preparation was performed according to Illumina guidelines for sequencing on HiSeq 2500 line.