maize Mo17 embryo biological rep 3
QC: Samples are quantified using fluorimetry (RiboGreen assay) and RNA integrity assessed using capillary electrophoresis (e.g., Agilent BioAnalyzer 2100), generating an RNA Integrity Number (RIN). For a sample to pass QC, mass must be = 1 ug, with a RIN > 8.    Library Creation: Steps include oligo-dT purification of polyadenylated RNA, and reverse trascription to create cDNA. The cDNA is fragmented, blunt-ended, and ligated to indexed (barcoded) adaptors. The library is size selected (in the case of paired-end RNA-Seq), size distribution validated using capillary electrophoresis, and quantified using fluorimetry (PicoGreen) and via Q-PCR.    Sequencing: Indexed libraries are then normalized, pooled (depending on number of reads requested), clustered on a flow cell, and loaded onto the instrument for sequencing.