GSM2487734: day 7 wheat straw rep3; Trametes coccinea; RNA-Seq
Total RNA was extracted from mycelia  harvested by filtering through Miracloth, squeeze dried, snap frozen in liquid nitrogen and stored at -80°C until use. Frozen fungal pellets were ground using the SamplePrep 6770 FreezerMill (Spex). RNA extraction was done from one hundred milligram of ground mycelium in 1 mL TRIZOL (Ambion). RNAs were precipitated with isopropanol, treated with DNAse I (QIAGEN) and resuspended in 25 µL RNAse Free water. RNA purity and integrity were analyzed on NanoDrop Spectrophotometer and Agilent 2100 BioAnalyzer. cDNA libraries were prepared using the TruSeq RNA-Seq Sample Prep Kit V2 (Illumina Inc., San Diego, CA), and submitted to sequencing using Illumina 2x75 bp technology (Beckman Coulter Genomics).