RNA-Seq of Rainbow trout low temperature stressor tank 18 run 1
Four unrelated families from the NCCCWA even year broodstock population under selective breeding for growth were used for each of the five stressors and control treatments. The fish were tagged and two fish from each family were placed in each of four 193 liter tanks on flow-through water. For each stressor the four tanks included three replicates and one control; a total of 160 fish were used. Total RNA was extracted from the gills of each individual fish. Equal amount of RNA from each fish was pooled by tank to prepare RNA-seq libraries. 