ITS1 and ITS2 of ITS region from Juglans nigra stem tissues
DNA was extracted from canker-bordering stem tissues. Extracted DNA was amplified for the ITS1 and ITS2 regions using a pool of six ITS3 and two ITS4 PCR primers. Sequencing was performed using the Illumina Miseq platform(2x250) at  UT Genomics Core (University of TN, Knoxville, TN)