RNA-seq of Cycas hainanensis: mature tree
Total RNA of each sample was isolated using TRIzol Reagent (Invitrogen) according to the manufacture’s instruction. RNA was DNase-treated using RNase-free (TaKaRa) to avoid genomic DNA contamination. The quality and quantity of total RNA were analyzed using an Agient 2100 Bioanalyzer (Agilent Technologies) and a Nanodrop ND1000 (NanoDrop Technologies)  respectively. The cDNA library was prepared for transcriptome sequencing using Illumina’s kit (Illumina). Then the cDNA libraries were sequenced on an Illumina HiSequation 2500 V4 kit platform to generate short sequencing reads of paired end (PE).