RNA-Seq of Macaca mulatta: adult male peripheral blood mononuclear cells
Total RNA was isolated from PBMCs using the mRNeasy kit (Qiagen, Valencia, CA). One microgram of poly(A)-enriched mRNA was fragmented followed by cDNA synthesis with random hexamers (New England Biolabs, Ipswich, MA). This product underwent end-repair, adapter ligation, and size selection using AMPure XP beads (Beckman Coulter Inc., Brea, CA) to isolate cDNA templates of 320 nucleotides that were amplified by PCR. Each library was prepared with unique index primers for multiplexing and subjected to single-end 100 bp sequencing on the HiSeq2500 platform (Illumina, San Diego, CA)