GSM4183369: Peritoneal macrophage, KO-lps-; Mus musculus; RNA-Seq
Total RNA from nonactivated peritoneal macrophages was quantified using a NanoDrop ND-1000 instrument, and 1 to 2 µg of total RNA was used to prepare the sequencing library with the following steps: (1) Total RNA was enriched by oligo (dT) magnetic beads (rRNA removed), and (2) the RNA-Seq library was prepared using the KAPA Stranded RNA-Seq Library Prep Kit (Illumina), which incorporates dUTP into the second cDNA strand and renders the RNA-Seq library strand-specific. RNA libraries were prepared for sequencing using standard Illumina protocols