GSM2753756: FHSC04_RG6016_5 (in vivo); Homo sapiens; RNA-Seq
Tumors were surgically excised from immunocompromised mouse host, with pieces of tumor frozen on dry ice and later used for total RNA extraction using TRIzol reagent. Tissue was minced with a scissors and then homogenized using a syringe/needle in 1mL Trizol reagent, with RNA isolated following manufacturers instructions. RNA libraries were prepared using theNEBNext Ultra RNA Library Prep Kit for Illumina (catalog E7530L). Briefly, 500 ng total RNA was used to isolate mRNA with the NEB Next Oligo dT Magnetic Isolation Module (catalog E7490L). The isolated mRNA undergoes fragmentation, priming, cDNA synthesis and end repair/dA-tail. Individual index primers for multiplexing were added during PCR library enrichment after adaptor ligation.