Archeal 16s gene for sample 1E
"Approximately 10 L of aquifer water were passed through duplicate 0.2 micro meter ""sterivex"" cartridge filters (Millipore, Darmstadt, Germany) that were immediately flash-frozen in liquid nitrogen and then stored at -80C until DNA extraction was performed using PowerWater DNA isolation kit (MoBio Laboratories, Carlsbad, CA) according to manufacturer instructions. Amplicon pyrosequencing performed at Molecular Research DNA labs (www.mrdnalab.com, MRDNA, Shallowater, TX,USA) using both the 16s eubacterial primer 27F and archaeal primer 349F"