GSM4676191: WT; Aspergillus nidulans; RNA-Seq
Total RNA from conidia was extracted using Trizol Reagent (Invitrogen, USA), according to the manufacturer's instructions with modifications. To remove DNA contamination from the RNA samples, DNase I (Promega, USA) was added and then RNA was purified using an RNeasy Mini kit (Qiagen, USA). RNA samples were submitted to the University of Wisconsin Gene Expression Center (Madison, WI, USA) for library preparation and sequencing. A strand-specific library was prepared using an Illumina TruSeq strand-specific RNA sample preparation system.