GSM2412128: WT-NS; Mus musculus; RNA-Seq
CD43- splenic B-cells from wt, Em-deficient and 3'RR-deficient mice were cultured in conventional growth medium alone or with 5mg/ml of LPS for 2 days. Cells from two different mice were pooled. Cellular RNA was then trisol-extracted. Libraries were generated from 1µg of total RNA using Truseq Stranded Total RNA kit (Illumina). Libraries were then quantified with KAPA library quantification kit (Kapa biosystems) and pooled. 4nM of this pool were loaded on a Nextseq 500 high output flowcell and sequenced on a NextSeq 500 platform (Illumina) with 2 × 150bp paired-end chemistry.