GSM2491565: Wild Type Cell 15; Mus musculus; RNA-Seq
single-cell isolation protocol: Individual cells were isolated for mRNA sequencing using the C1 Single-Cell Auto Prep System (Fluidigm). imageing protocol:  Each cell isolation chamber was imaged at 20X (Leica DMI 6000) with bright-field illumination and fluorescent channels. The Ter119 antibody was labeled with PCP-Cy5.5 and imaged using a 488/705 nm (excitation/emission) fluorescence filter cube. Cells were lysed, their mRNA reversely transcribed into cDNA, and the cDNA amplifed using the SMART-Seq whole-transcriptome amplification method on the C1 System according to the manufacturer's instructions. The cDNA libraries were collected from the C1 and prepared for sequencing using a Nextera XT DNA Sample preparation kit (Illumina).