GSM2574361: 4su01; Homo sapiens; ncRNA-Seq
Illumina TruSeq stranded kit omitting polyA selection step on 4sU-containing RNAs enriched from total RNA. Small RNA and 4sU-incorporated RNA extracted from cells using Trizol. DNA for DRIP-seq obtained by extracting the nuclear fraction and treating with protease, fragmented by in vitro AsiSI digestion plus sonication, then DNA:RNA fragments isolated by S9.6 antibody