GSM4073662: DC_A_9; Homo sapiens; RNA-Seq
RNA was extracted from 4 hrs stimulated cells. Total RNA concentration was measured, checked for quality and used for cDNA preparation by reverse transcription of 5.6 μL of purified RNA. cDNA was amplified using Smart-seq2 protocol (Picelli et al., Natue protocols 2014), purified, quantified, and checked for quality and library size. Nextera XT DNA library preparation kit  (Illumina, FC-131-1096) was used with 1 ng purified cDNA for the construction of swquencing libraries. cDNA libraries having insert sizes of 500-800 bp were used for paired-end sequencing on the Illumina NovaSeq 6000 sequencing platform (Novogene Corporation, China).