Genomic DNA sequencing of Ixodes scapularis clone ISE6 Cell Line
(1) Library Sizing by Pippin Prep.(2) NextGen Paired End Barcoded Genomic Library Construction.(3) NextGen Library quantification and normalization by qPCR.  Genomic DNA was isolated from the cell line using a Qiagen genomic DNA isolation kit.  Bioanalyzer analysis confirmed high molecular DNA was recovered.  Sample was sequenced on Next Generation Sequencing platforms.  Reads were demultiplexed which removed barcodes and sequencing adapters.