GSM4224708: H2009 RUVBL1 PCRmutant +dox rep3; Homo sapiens; OTHER
Cells harvested via trypsinization and then genomic DNA was isolated using Qiagen Blood & Cell Culture DNA Mini Kit RUVBL1 or RUVBL2 cDNAs were amplified via nested-PCR with primers containing a unique barcode for each sample. Adapators and sequencing barcodes were added using the KAPA Hyper Prep Kit DNAsequencing using MiSeq