GSM4432487: CD1 MNP 6; Mus musculus; RNA-Seq
Mice were euthanized according to established protocols. The embryos were harvested and washed extensively with ice cold Depc treated PBS. Maxillary prominence, Mandibular prominence, Frontonasal prominence, limb, dorsal brain and ventral brain were dissected from the embryos and samples from multiple embryos were pooled for each tissue. The isolated tissue samples were homogenized using a tissue homogenizer and total RNA was extracted using Trizol (Fisher Scientific) following manufacturer's instructions. After quality control validation, samples were submitted for NGS sequencing. Six independent biological replicates were used for each tissue Paired end sequencing was performed for each sample with a minimum of 20 million reads per library with a 75bp read length. All library preparation and sequencing were performed at the DNA sequencing core at CCHMC using standard illumina sequencing protocols.