GSM4676822: MPP_MTP1; Mus musculus; RNA-Seq
Total RNA was isolated using a Direct-zol RNA Kit (Zymo Research) with DNase treatment. RNA with RIN greater than 7 underwent polyA selection. Insect carrier RNA Was added to allow low-input RNA library prep. Furthermore, samples were reverse transcribed to cDNA. cDNA libraries were prepared using 50-100ng of total RNA using the Nextflex Rapid Direction RNA-Seq kit (Perkin-Elmer).