GSM3995466: WtPlankGlycerol/259_PG#; Yersinia pestis; RNA-Seq
Bacteria were resuspended in RNA protect and extracted using RNA-whiz and precipitated in isoproponol. RNA was treated with Turbo Dnase Total RNA was checked for quality and quantity on Agilent Bioanalyzer 2100 and Qubit fluorometer. The Illumina TruSeq Stranded Total RNA Library Preparation Kit was used to prepare sequencing libraries from 500 ng of total RNA samples according to manufacturer's instructions without modifications. This procedure includes rRNA depletion with Ribo-Zero rRNA Removal Kit (Bacteria), remaining RNA purification and fragmentation, cDNA synthesis, 3'-end adenylation, Illumina adapter ligation, library PCR amplification and validation. Illumina NextSeq 500 Sequencer was used to sequence the libraries with the production of single-end, 75 bp reads.