GSM4663395: Healthy Control 2; Homo sapiens; RNA-Seq
Total RNA of plasma was extracted using TRIzol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. mRNA was fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final DNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (±50 bp).