GSM3933507: Control IP Elution Rep1; Homo sapiens; RIP-Seq
After cell harvesting by scraping, membranes were isolated by hypotonic lysis followed by low speed centrifugation. For samples SAA1-6, membranes were then digitonin solubilized, followed by Flag IP and ultracentrifugation to isolate TMCO1-attached ribosomes. RNA libraries were prepared for sequencing using standard Illumina protocols, using RiboZero to deplete rRNA in samples SAA1-6 and poly-dT in samples RKAA1-10.