RNA-seq of  MC38 wild type tumor
RNA was  extracted using RNeasy mini kit (Qiagen Cat. No. 74104). Quality of samples  was checked by Bioanalyzer 2100 (Agilent) and RNA was quantified by Nanodrop.  After, 5 g of RNA was enriched by NextFlex Rapid Directional mRNA-Seq  (Bioo-Scientific) kit. Quality of samples were checked again by Bioanalyzer  2100 (Agilent) and quantified by qPCR. "Samples were put on Flow Cell  High Output. Amplification and sequencing were performed with Illumina  NextSeq500: run Paired-end (2*75nt) for all MB49 tumor and run Single Read 75  bp for MC38."