GSM4563408: Transgenic ZT12-2 [TG12-2]; Mus musculus; RNA-Seq
Gastrocnemius muscle was removed, and total RNA was isolated using RNeasy mini kit (Invitrogen). Polyadenylated mRNA was separated from 100 ng of total RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB). Sequencing libraries were generated using NEBNext Ultra RNA LibraryPrep kit for Illumina (New England Biolabs) according to manufacturer's instructions. Library concentrations were assessed by both a Bioanalyzer (Agilent) and NEBNext Library Quant Kit for Illumina (New England Biolabs).