GSM4564595: Input_ev; Caenorhabditis elegans; ChIP-Seq
Worms were harvested and washed with M9 buffer for three times. Worms were then fixed with 1% formaldehyde in PBS for 30 min, and quenched by glycine. Immunoprecipitaion were carried by using the antibodies against H3K18Ac (Cat. 07-354, Merck) or H3K27Ac (Ab4729, abcam). Libraries were prepared for sequencing using standard BGISeq500 protocol