Draft genome sequence of crotenoid producing Planococcus maritimus MKU009 strain
Library preparation was performed at Genotypic Technology’s Genomics facility following NEXTFlex  DNA library protocol outlined in “NEXTFlex DNA sample preparation guide (Cat # 5140-02).  In brief, genomic DNA was sheared to generate fragments of approximately 300-500bp in a Covaris  microTube with the S220 system (Covaris, Inc., Woburn, MA, USA). The fragment size distribution  was checked using Agilent Tapestation with D1000 DNA Kit (Agilent Technologies) according to the  manufacturer’s instructions. The resulting fragmented DNA was cleaned up using HighPrep beads  (MagBio Genomics, Inc, Gaithersburg, Maryland). These fragments were subjected to end-repair, A-  tailing, and ligation of the Illumina multiplexing adaptors using the NEXTFlex DNA Sequencing kit as  per the manufacturer’s instruction. The resulting ligated DNA was cleaned up using HighPrep beads  (MagBio Genomics, Inc, Gaithersburg, Maryland) and size selected (400–600bp) on 2% low melting  agarose gel and cleaned using MinElute column (QIAGEN, India). These adapter ligated fragments  were subjected to 10 rounds of PCR (denaturation at 98 ̊C for 2 min, cycling (98 ̊C for 30sec, 65 ̊C for  30sec and 72 ̊C for 1 min) and final extension at 72 ̊C for 5 min) using primers provided in the  NEXTFlex DNA Sequencing kit. The PCR products were purified using HighPrep beads. Quantification  and size distribution of the prepared library was determined using Qubit flourometer (Table 1) and the  Agilent High Sensitivity DNA Kit (Agilent Technologies) respectively according to the manufacturer’s  instructions