GSM4498895: WT+AAV9-GFP Replicate 3; Mus musculus; RNA-Seq
Lumbar DRGs were isolated from uninjected and either AAV9-GFP or AAAV9-SMN injected wild type mice at P300 and flash frozen in dry ice. Total RNA was isolated using RNAqueous-Micro Kit (Ambion) including DNaseI treatment. RNA quality and quantity were assessed using the 2100 Bioanalyzer (Agilent). We performed ribosomal depletion to remove rRNAs from total RNA samples, then proceeded with library construction using Illumina TruSeq chemistry. Libraries were sequenced using Illumina NovaSeq 6000. Libraries were multiplexed and sequenced on Illumina NovaSeq 6000 to generate paired-end 100bp reads for each sample.