GSM2491466: Tg-9101-Derm; Mus musculus; RNA-Seq
Skin wound tissues (2 days after scab detachment) of control and K14-CreERT2; LSL-Shh mice and embryonic skin (E14.5d) were incubated in 20 mM EDTA solution at 37 oC for 2 hrs. After separation of epidermis from dermis, total RNA were isolated from epidermis and dermis using RNeasy Plus Micro-Kit (Qiagen) as described by manufacturer. RNA libraries were prepared for sequencing using standard Illumina protocols