GSM4072408: UCN2; Homo sapiens; RNA-Seq
AD-MSCs and UC-MSCs were cultured for 2 weeks with medium changed twice weekly. Serum was obtained from mice who received DSS for 7 days. AD-MSCs and UC-MSCs were cultured with serum (final concentration: 10%) from DSS-induced colitis model mice and compared with AD-MSCs and UC-MSC treated with serum (final concentration, 10%) from normal mice. After 48 h, cells were harvested, and mRNA expression levels were analyzed. First strand cDNAs were transcribed to the 5' end of capped RNAs, attached to CAGE "bar code" tags.