GSM3860403: MCF7_dEnh_RNA_2; Homo sapiens; OTHER
Total RNA was extracted using Qiagen Rneasy mini kit and treated with Dneasy prior to library construction ATAC-seq: Nuclei were isolated from tissues using gradient centrifugation as per the Omni-ATAC and were exposed to the transposase, Tn5, prior to llibrary construction. RNA libraries were prepared using standard Illumina TruSeq protocols ATAC-seq libraries were prepared for sequencing using standard Illumina Novaseq 6000 riboRNA-depleted TruSeq stranded library was sequenced using Illumina HiSeq 2500 with 125bp paired-ended sequencing. !Sample_extract_protocol_ch1 = ATAC-seq library was sequeced using Illumina Novaseq 6000 with 50bp paired-ended sequencing.