GSM3614729: enriched_U-DNA_2; Homo sapiens; OTHER
Genomic DNA was purified by Quick-DNA Miniprep Plus Kit (Zymo Research) and sonicated with a BioRuptor (Diagenode). Immunoprecipitation was carried out by FLAG-tagged sensor protein binding to uracil residues in fragmented genomic DNA and was followed by purification with anti-FLAG beads. DNA libraries were prepared for sequencing using standard Illumina protocols.