GSM3861162: HPP_15_523_11_AB_YS; Rattus norvegicus; RNA-Seq
Embryos or visceral yolk sacs were placed in 500 ul RNAlater (Ambion) for total RNA extraction using standard methods. The RNA was prepared for sequencing using the Tru Seq™ Sample Preparation Kit (Illumina, Part #15026486) according to the manufacturer's protocols. For each sample, 500 ng of total RNA was used to prepare the cDNA libraries. RNA Seq libraries were sequenced for 50‐bp pair-end reads on an Illumina HiSeq 2000 using the TruSeq SBS Kit (Illumina, Version #3) by IQVIA Institute for Human Data Science, formerly Expression Analysis, Inc. (RTP, NC, USA).