NextSeq 500 sequencing; Metatranscriptome of surface gravel plain soils from the hyperarid Central Namib Desert
Metatranscriptome of surface gravel plain soils from the hyperarid Central Namib Desert
Soil preservation in RNAlater solution Natural conditions No treatment CTAB lysis buffer (5% CTAB, 0.7 M NaCl, 240 mM KH2PO4, pH 8), TRIzol extraction ScriptSeq v2 Complete Gold Kit (Epidemiology)(Epicentre), ScriptSeq Index PCR primers (Set 1) (Epicentre)