GSM4188538: DNT non-transgenic seeds; Nicotiana tabacum; RNA-Seq
Total RNA samples were prepared by the LiCl method from 3-week old seedlings (in the 35:A9 experiment), or from seeds (in the DSA10:A9 and DS10:A4a experiments) Library preparation and sequencing on an Illumina HiSeq 4000 sequencer using 100-bp paired-end reads protocol were performed by BGI Co., Ltd (https://en.genomics.cn/). Agilent 2100 Bioanaylzer and ABI StepOnePlus Real-Time PCR were used in quantification and in qualification of the sample libraries.