Illumina HiSeq 2500 paired end sequencing; RNA-seq of Zrsr1-2WT and Zrsr1-2 mutant embryos
RNA-seq of Zrsr1-2WT and Zrsr1-2 mutant embryos
Recovery of embryos at 1 dpc Total RNA was extracted with Arcturus Pico Pure RNA Isolation Kit (Molecular Devices, USA) prior to the preparation of the libraries for sequencing.  RNAseq libraries were prepared from pools of 100 2cell-embryos (3mu / 2 WT) according to the manufacturer's protocol.