GSM4224316: HeLaS3 AGO2KO; Homo sapiens; RNA-Seq
Fixed cells were prepared for the Drop-seq run by centrifugation (1000 g, 5 min) and resuspension in 1 ml of PBS-BSA 0.01% + RiboLock (ThermoFisher) (0.8 U/µl), followed by another centrifugation (1000 g, 5 min) and resuspension in 0.5 ml of PBS-BSA 0.01% + RiboLock (ThermoFisher) (0.8 U/µl). Cells were then passed through a cell strainer (35 µm), counted, diluted with PBS-BSA 0.01% to a final concentration of 100 cells/µl and transferred into a syringe to be loaded on the Drop-seq apparatus. Alles et al BMC Biol 2017 doi: 10.1186/s12915-017-0383-5. single-cell RNAseq