GSM4072643: Biozzi Con 1 [MBC1]; Mus musculus; RNA-Seq
Spinal cord tissue was extracted and dissociated to single cell suspension using the Neural Tissue Dissociation Kit (Milteny). Myelin was removed using Percoll (GE Healthcare) followed by cell isolation using CD11b microbeads (Milteny) and MS columns (Milteny) according to manufacturer instructions. RNA was isolated using RNeasy Plus Mini Kit (Qiagen). Total RNA samples were enriched for mRNAs by pull-down of poly(A)+ RNA using PolyA Kit (Truseq). RNA-seq libraries were prepared from 100ng RNA using the SENSE Kit (lexogen).