GSM4186902: a2B1; Homo sapiens; RNA-Seq
RNA was isolated from 100,000 unexpanded, CPI203 (150nM) or vehicle (0.1% DMSO) expanded cord blood CD133 cells using RNeasy Plus Mini Kit (QIAGEN) according to the manufacturer's protocol. mRNA capture and cDNA synthesis was using KAPA mRNA Hyper Kit for Illumina platform (KAPA) according to the manufacturer's protocol. After quality check using Qubit High-sensitivity kit (Life Technologies), 16 cycles of PCR was performed for amplifying each library. Each experiment starting from cell expansion was performed duplicately using cells from three different biological donors.