GSM4564569: Mbp1_1-561_Replicate_1; Saccharomyces cerevisiae; MNase-Seq
cells were crosslinked with 1% formaldehyde for 20 min, spheroplasted, digested with micrococcal nuclease, quenched, treated with RNase for 40 minutes, crosslinks reversed for 1 hour at 65 degrees with proteinase K treatment, purified by MinElute (PCR clean-up) column, CIP-treated, and mononucleosome band was gel-extracted CIP-treated nucleosome-sized DNA footprints were converted to libraries with NuGEN Ovation Ultralow kit