RNA-seq of PA14 small RNA
For small RNA-seq, around 300 nanograms of  small RNA from each sample was first treated with RNA 5' Pyrophosphohydrolase  (New England Biolabs, MA) at 37oC for 30 minutes, then converted to Illumina  sequencing libraries using the PrepX RNA-seq library preparation protocol on  the automated Apollo 324TM NGS Library Prep System (Takara Bio, CA).