GSM1893033: hat1u2_15109; Candida albicans; RNA-Seq
RNA was isolated using Trizol and glass bead lysis after which RNA was precipitated and DNase treated followed by a column purification step. 5μg total RNA was depleted of rRNAs using the RiboMinus Eukaryote System v2 (Invitrogen), rRNA-depleted RNA was fragmented using the NEBNext Magnesium RNA Fragmentation Module (NEB). First strand cDNA was synthesized with the SuperScript III Reverse Transcriptase (Invitrogen) and second strand synthesis was done using the NEBNext mRNA Second Strand Synthesis Module (NEB) according to the manufacturer’s instructions. Library preparation was done using the KAPA DNA Library Preparation Kit (Kapa Biosystems) according to the manufacturer’s instructions.