DNA-Seq of diesel exhaust-adapted E. coli T56-1
Genomic DNA was extracted from M9-glucose E. coli T56-1 culture. The genomic libraries were generated with NEB Next Ultra DNA Library Prep Kit for Illumina (NEB, MA). After ligation of the adapters, DNA fragments ranging from 300 to 400bp were recovered by beads purification. Sequencing was done with an Illumina HiSeq 4000 (Illumina, CA) with a paired-end protocol and read lengths of 150 nucleotides. The reference genome for the analysis was Escherichia coli BW25113 (NCBI reference sequence NZ_CP009273.1).