Arabidopsis thaliana ecotype Col-0 mutant jah2 DNA sequencing
All Arabidopsis thaliana plants were of the Col-0 ecotype. The jah2 mutant originated from a population of ethyl methanesulfonate (EMS) mutagenized M2 seeds purchased from Lehle Seeds (Round Rock, Texas). This mutant was recessive, based on analysis of F1 and F2 generations from a cross to WT and all experiments were done using jah2 seed obtained after 3 backcrosses to Col-0. Seedling growth on MS agar medium was as previously described (Staswick and Tiryaki, 2004) in an incubator at 22C, 12 h day/night cycles, 150-200 µM s-1 m-2. Fertilization was done weekly using Miracle grow complete fertilizer in water, as recommended. Tissue for thiol determination was from rosettes of soil-grown plants (5 weeks, not bolting) frozen in liquid nitrogen and then ground to a fine powder.  DNA extraction and library prep via manufacturer's protocol (Illumina).