WXS of Human: 76 y.o. carotid paraganglioma male patient
The sections from paraffin-embedded tissues were prepared on glass slides, and then stained with hematoxylin-eosin (H&E). Tumor areas were chosen in unstained sections according to the reference H&E slide. DNA was isolated using the High Pure FFPET DNA Isolation Kit (Roche, Switzerland). Library preparation for exome sequencing was carried out using the Nextera Rapid Capture Exome Kit (Illumina, USA). Sequencing was performed using the NextSeq 500 System (Illumina, USA). At least 60 million paired-end reads of 75 nucleotides in length were obtained for each sample with 300x minimal coverage.