GSM3389600: A673_WT_input_A350C18; Homo sapiens; ChIP-Seq
DNA-protein cross-linking was done in presence of 1% of paraformaldehyde on 12 × 10^6 cells for each condition for 10 minutes. Cell lysis, chromatin shearing, immunoprecipitation and DNA purification was performed with reagents from iDeal ChIP-seq kit for Transcription Factors (Diagenode, ref: C01010054). Chromatin shearing was carried out in a Bioruptor (Diagenode) using 20 cycles of sonication (30sec high, 30sec off) in TPX tubes (Diagenode, ref: 50001). For immunoprecipitation of MYBL2, 2 µg of a monoclonal ChIP-grade rabbit anti-MYBL2 antibody (Abcam, ref: 76009) were used. IgG and CTCF ChIP was included as negative and positive control, respectively. MYBL2 ChIP and input were sequenced on a HiSeq2500 instrument (Illumina) using 100bp single-end sequencing.