Bighorn sheep genome sequencin WhiskeyBasin_old
DNA library construction, 50 ul of 127 pooled DNA was sheared to an average insert length of 300-400 bp using a Covaris S220 128 (Woburn, MA, USA). Short insert libraries were prepared as described by the Illumina TruSeq 129 sample preparation guide v2 (Illumina Inc, San Diego, CA). Sequencing was performed on an 130 Illumina HiScan machine to generate 100 bp paired end reads.