Illumina HiSeq 2000 paired end sequencing; Illumina HiSeq next -generation sequencing of telomere fusion amplicons purified from crisis-stage MCR5HPVE6E7 and TALEN-transfected HCT116 cell lines at 48 and 120 hours post-transfection.
Illumina HiSeq next -generation sequencing of telomere fusion amplicons purified from crisis-stage MCR5HPVE6E7 and TALEN-transfected HCT116 cell lines at 48 and 120 hours post-transfection.
'Cell lines were cultured at 37◦C in 5% CO2 in McCoy''s 5A medium supplemented with 10% (v/v) foetal calf serum, 1x10^5 IU l^-1 penicillin, 100 mg l^-1 streptomycin and 2mM glutamine, as well as antibiotic selection agents as required (2.5μg/ml G418 for LIG3-/- and 2.5μg/ml G418 and 2.5μg/ml puromycin for LIG3-/-:NC3).' Illumina HiSeq 2000 paired-end sequencing of the MCR5HPVE6E7 fusion amplicons was performed by BGI using standard library preparations.