RNA-Seq of mus musculus: adult male liver
DNase I and RNase H were used to remove DNA and ribosomal RNA (rRNA), respectively. The purified rRNA-depleted RNA was fragmented into small pieces by incubating in fragmentation buffer at 94 C for 5 min. The first and second strands of cDNA were generated using N6 random primer by PCR and the product was purified by magnetic beads and heat denatured. The final samples were sequenced at the BGISEQ-508 platform (BGI, Shenzhen, China). The raw reads were filtered to discard the unknown nucleotides and low-quality reads, and then the clean reads were mapped onto known C. hongkongensis transcriptome datasets. The FPKM (fragments per kilo base of transcript per million mapped reads) was used to estimate the abundance of unigenes.