GSM2410979: Complete-media; Cercospora zeina; RNA-Seq
Cellophane sheets with Cercospora zeina growth were removed from the respective growth media plates and flash frozen in liquid nitrogen. For the liquid growth media, the C. zeina cultures were filtered through Whatmann’s nr 1 filter paper and the mycelia flash frozen. Mycelia was ground in liquid nitrogen and 3g used for total RNA isolation using QIAzol Lysis Reagent (Qiagen) as per the manufacturer’s specifications. RNA purification and DNA digestion of 100µg of total RNA was performed using the RNeasy Mini Kit and the RNase-free DNase set for on-column DNA digestion (Qiagen) as per the manufacturer’s specifications. Libraries were constructed according to the Illumina manufacturer's protocol by the sequencing service laboratory (BGI).