Ty1 Integration Data
Independent Ura+ transformants that supported a robust induction of Ty1HIS3-SCUF transposition were identified by growing patches of each isolate on SC-Ura 2% galactose 2% raffinose agar at 20°C followed by replicating patches to 5-FOA-His plates. Selected pGTy1his3AI-SCUF transformants were grown overnight in SC-Ura 2% glucose broth at 30°C. A 10µl aliquot of each culture was transferred to 1 ml SC-Ura 2% galactose, 2% raffinose 2% sucrose broth, and cultures were grown at 20°C for 2 days. Cells were pelleted, resuspended in 0.2 ml ddH20, transferred to YEPD agar and incubated at 30°C for 16-18 hr. Cells were replicated to 5-FOA-HIS 2% glucose plates and incubated at 30°C for two days. A 0.75 ml aliquot of ddH20 was added to each plate, and cells were scraped from the agar into suspension using a sterile plastic scraper. The cell suspension was collected, and the agar surface was washed with an additional 0.75 ml aliquot of ddH20. The cell suspensions were combined, and cells were pelleted; approximately 0.1 ml of cell pellet was obtained from each plate. Genomic DNA was prepared from individual pellets. Genomic DNA samples from 10 to 14 independently prepared cell pellets in each strain background were used for PCR.   PCR amplification of the integration sites was based upon the linker mediated PCR protocol found in (Baller et al. 2011). Each sample was split, one fraction digested with AciI and the other with TaqI. Linkers were annealed and sequences with an adjacent Ty1 insertion were amplified by PCR.  Barcoded primers were used in the PCR step to mark the source of the sequences