GSM1522564: Input DNA GBM1A; Homo sapiens; ChIP-Seq
Cells were subjected to ChIP using MAGnify ChIP system (Invitrogen) following manufacturer’s protocol. KLF9 bound DNA was immunoprecipitated using mouse anti-FLAG M2 antibody (Sigma-Aldrich) and Dynabeads Magnetic Beads (Life Technologies). ChIP-enriched DNA was used for PCR and deep sequencing. ChIP-enriched DNA or input DNA (10ng each) was subjected to library preparation using ChIP-Seq DNA Sample Prep Kit (Illumina) following manufacturer’s protocol.