GSM2576823: rna_rct1_1; Schizosaccharomyces pombe; RNA-Seq
ScriptSeq V2 kit (Epicentre) was used to prepare barcoded RNA-Seq libraries. 50 ng ribosomal RNA (rRNA)-depleted RNA samples were used as starting material following manufacturer's protocol. Ribo-Zero Gold kit (Epicentre) was used to remove rRNA from total RNA (DNA free) samples. Barcoded libraries were pooled and sequenced on Illumina HiSeq platform.