GSM4188957: Ncstn KO 1; Mus musculus; RNA-Seq
Dissected urothelia seperated from muscularis propria were frozen in liquid nitrogen, pulverized with a mortar and pestle and total RNA was isolated ising TRI-Reagent (Merck). Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 0.5 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols