GSM2492349: 8F3; Trichodesmium erythraeum IMS101; RNA-Seq
RNA was extracted using the Ambion MirVana miRNA Isolation Kit (Thermo Fisher Scientific) in an RNAse free environment according to the manufacturer's instructions followed by Ambion's Turbo DNA-free kit to degrade trace amounts of DNA. Extracted RNA was sent to the UC San Diego IGM Genomics Center for library construction and Illumina sequencing. Briefly, rRNA was removed from total RNA using the Ribo-Zero rRNA Removal Kit (Illumina), and libraries were constructed with the TruSeq Stranded mRNA Library Prep Kit (Illumina) followed by 50 base pair, single end sequencing with the Illumina HiSeq4000.