GSM4675274: Tumor T61; Homo sapiens; RNA-Seq
Cells were lysed and RNA was extracted and converted to cDNA and tagged with barcodes following the 10x Genomics Single Cell 3' Protocol utilizing the ChromiumTM Single Cell 3' v2 Library & Gel Bead Kit and ChromiumTM Single Cell A  Chip Kit (10x Genomics, Pleasanton, CA). Barcoded cDNA was sequenced using an Illumina HiSeq 2500 flow cell with Rapid chemistry and 100 bp paired end sequencing, producing an average of 235 million reads per patient sample with an average of 11,502 cells/sample and 23,000 reads/cell