GSM2571019: HEK293T_untreated; Homo sapiens; RNA-Seq
Cells from a confluent 100mm dish were harvested in 1ml QIAzol Lysis Reagent (Qiagen, 79306). Total RNA was extracted using Qiagen RNeasy Mini Kit (Qiagen, 74106). Messenger RNA was purified from 5µg total RNA using NEB magnetic mRNA Isolation Kit (NEB, S1550S) and eluted into a final volume of 25µl. Libraries were prepared using 9µl of isolated mRNA and ScriptSeq RNA-Seq Library Preparation Kit (Epicentre, SSV21124).