HiSeq X Ten paired end sequencing of SAMD00198545
The library for ddRAD-Seq (Peterson et al. 2012) was created with slight modifications. The protocol used in the present study is exactly the same as that reported in Sakaguchi et al. (2015). In brief, we used the restriction enzymes EcoRI and BglII (New England Biolabs, Ipswich, MA, USA) for restriction digestion during the library preparation. After the PCR amplification of the DNA, the sequencing libraries were purified with AMPure_XP (Beckman Coulter, CA, USA) and size-selected around 380bp under gel electrophoresis using E-Gel_ SizeSelectTM (Life Technologies, Carlsbad, CA, USA). After quality measurement using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), the library was sequenced with 150+150bp paird-end reads in a total of three lanes of an Illumina HiSeqX (Illumina, San Diego, CA, USA) by Macrogen (Seoul, South Korea).