CTRL6
Total RNA (500 ng) was converted to cDNA libraries using TruSeq Stranded Total RNA with Ribo-Zero kit (Illumina) according to manufacturer's protocol. Libraries were evaluated qualitatively on Bioanalyzer 2100 using High Sensitivity DNA Kit and quantitatively on CFX96 Real-Time PCR system (Bio-Rad) using KAPA Library Quantification Kit (Kapa Biosystems), and then sequenced 2x76bp on HiSeq2500 Illumina platform. At least 25 M reads per sample were obtained with mean quality score (>=Q30)>94%.