GSM2753790: RNA seq PDGFRbCre;WT1_+_GFP;Ai14 control, GFP low #1268/1802; Mus musculus; RNA-Seq
Hepatic stellate cells were isolated from livers by density centrifugation and subpopulations sorted by flow cytometry into RLT with beta-mercaptoethanol. Total RNA was isolated using Qiagen RNeasy columns following the manufacturer's instructions. RNA libraries were prepared for sequencing using standard Illumina protocols