RNAseq of human RDEB skin keratinocytes
Total RNA from 80-90% confluent keratinocytes was isolated using the RNeasy Mini Kit. Libraries were constructed with the stranded TruSeq GOLD protocol (RS-122-2301) followed by rRNA depletion (Illumina Ribo-Zero rRNA removal kit human, mouse, rat). Sequencing was performed on a HiSeq2500 (50 bp, Illumina, San Diego, USA).