GSM1524372: T29_MethylC-seq_rep1; Homo sapiens; Bisulfite-Seq
Genomic DNA were extracted from T29 cell lines by Qiagen DNeasy Blood & Tissue Kit (Qiagen, Germany) and 20 mg/mL RNase (Qiagen, Germany) was used to degrade contaminated RNA in DNA samples. DNA integrity was verified by agarose gel electrophoresis. Quantity of DNA was measured using Quant-iT PicoGreen dsDNA Kits on Qubit 2.0 (Invitrogen, USA) Final libraries were quantified by Quant-iT PicoGreen dsDNA Kits (Invitrogen, USA) and Library Quantification Kit-Illumina/Universal (Kapa Biosystems, Inc. Woburn, MA) and sequenced on Illumina HiSeq 2000