GSM4432420: Org_8wk Rep1 RNA-seq; Homo sapiens; RNA-Seq
Cultured samples were directly lysis into RLT plus buffer and total RNA were extracted using RNeasy plus Micro Kit (Qiagen) Organoids were flash frozen on dry ice, and RNA was harvested using the RNAeasy Micro Kit from Qiagen. Whole transcriptome Illumina TruSeq Stranded Total RNA Library Prep Kit Gold (Illumina) was used for the constuction of libraries Cultured samples were directly lysis into RLT plus buffer and total RNA were extracted using RNeasy plus Micro Kit (Qiagen) TruSeq Stranded Total RNA kit Gold (Illumina) or Whole transcriptome Ovation Solo RNA seq Library Preparation Kit (TECAN, NuGEN) was used for the library preparation of all samples