GSM2574365: DRIP02; Homo sapiens; ChIP-Seq
Libraries constructed using the NEBNext Ultra II kit following the manufacturer protocol. 1ng of DRIP sample was used. 8 PCR cycles were used and an additional 0.85X volume clean-up using the supplied sample purification beads. Small RNA and 4sU-incorporated RNA extracted from cells using Trizol. DNA for DRIP-seq obtained by extracting the nuclear fraction and treating with protease, fragmented by in vitro AsiSI digestion plus sonication, then DNA:RNA fragments isolated by S9.6 antibody