GSM2412140: O1; mixed sample; RNA-Seq
One million non-irradiated, P or X irradiated CAL33 cells (CR-MI group) were injected subcutaneously into the flank of 6-week-old NMRI-Foxn1nu/Foxn1nu female mice (Janvier Labs, Le Genest-Saint-Isle, France, n=10/group). The tumor volume (v = L x l2 x 0.52) was determined following measurement with a caliper. When the tumors reached one cm3, the mice were sacrificed and the tumors collected. Total RNA was extracted from tumors with the AllPrep® DNA/RNA/Protein Mini Kit (Qiagen, Hilden, Germany). Libraries were generated from 1µg of total RNA using Truseq Stranded mRNA kit (Illumina). Libraries were then quantified with KAPA library quantification kit (Kapa biosystems) and pooled. 4nM of this pool were loaded on a Nextseq 500 high output flowcell and sequenced on a NextSeq 500 platform (Illumina) with 2 × 75bp paired-end chemistry