GSM4502376: BMMERT-act-unstim1; Mus musculus; RNA-Seq
For RNAseq analysis 0.5x10e6 BMM were stimulated with 2 µg/ml plate-coated TDM or evaporated isopropanol as solvent control for 24 hours. Total RNA was then isolated using the PeqGold RNA Micro Kit (Peqlab Biotechnology GmbH, Erlangen, Germany) according to the manufacturer's guidelines. RNAseq library preparation was done with pooled technical replicates using the TruSeq stranded mRNA Library Prep Kit (Illumina, Inc.)