GSM2411667: MPP subset 4; Mus musculus; RNA-Seq
Cells were encapsulated using the inDrops platform, into droplets on ice and lysed in the 4nL microfluidic droplets using a final concentration of 0.4% NP-40. Single cell lysates were subject to reverse transcription at 50°C without purification of RNA (as previously described in Klein et al., Cell 2015). Libraries were prepared as in Zilionis et al., Nature Protocols (in press), with the exception of the first round of libraries, for which the final PCR step was carried out as in Klein et al., Cell 2015.