GSM3389571: RNA-seq WT mid-log phase rep 2; Salmonella enterica subsp. enterica serovar Typhimurium str. LT2; RNA-Seq
Three milliliters of the cells cultured up to the exponential phase and 6 ml of RNAprotect Bacteria Reagent (Qiagen) were mixed immediately by vortexing for 5 sec, followed by 5 min incubation at room temperature and, after, centrifuged at 5000x g for 10 min. The supernatant was extracted, but for full supernatant removal the tube was inverted once onto a paper towel. Isolation of total RNA samples was conducted with RNeasy Plus Mini kit (Qiagen). The quantity was determined by Nanodrop 1000 spectrophotometer (Thermo Scientific). The quality was checked using RNA 6000 Pico Kit using Agilent 2100 Bioanalyzer (Agilent). Paired-end, strand-specific RNA-seq library was built with the help of KAPA RNA Hyper Prep kit (Kapa Biosystems) The RNA libraries were prepared for sequencing based on Illumina standard protocols