GSM3614873: RNA.mock2.day2; Homo sapiens; RNA-Seq
Cells were lysed in TCL buffer (Qiagen, Hilden, Germany) supplemented with 1 % 2-mercaptoethanol. HCV RNA was co-amplified with cellular mRNA using a SMART-compatible primer (sequence: 5'-Biotin-AAG CAG TGG TAT CAA CGC AGA GTA CTC TGC GGA ACC GGT GAG TA-3')23. Cellular mRNA was isolated and analyzed according to the SmartSeq2 protocol.