GSM4502477: Chicken_heart_scRNAseq_D7_RV; Gallus gallus; RNA-Seq
Embryos and hearts were isolated aseptically and placed in ice cold Hank's Balanced Salt Solution. To extract ventricular chicken cells, day 10 and day 14 stage samples were digested for four cycles of 10 minutes in 300U/mL collagenase type II at 37 degrees Celsius while Day 4 and day 7 stage samples were digested for one cycle of 20 minutes and once cycle of 10 minutes in 1.5mg/mL collagenase type II/dispase at 37 degrees Celsius. Samples were then passed through a 40 um cell filter, centrifuged, resuspended in ammonium chloride potassium red blood cell lysis buffer, centrifuged again, resuspended in phosphate buffered saline with 0.04% bovine serum albumin at 1 x10^6 cells/mL, and used for library constrction. 10x Genomics Chromium Next GEM Single Cell 3' RNA-seq Library Construction V3 Kit