NextSeq 500 sequencing
MuSCs were isolated from muscles of WT animals as described by Guenther et. al (Cell Stem Cell. 2013 Nov 7;13(5):590-601). MuSCs were transduced with Lentivirus encoding V5-tagged Duxbl for 12 hours and subsequently grown in proliferation medium (DMEM,  20%FCS; Pen/Strep; 2.5 ng/ml bFGF). Emerging SC Duxbl clones were then extracted and further processed.
For RNA-seq, RNA was isolated from SC Duxbl clones using the miRNeasy Micro Kit   (Qiagen). Total RNA and library integrity were verified on LabChip Gx Touch 24 (Perkin Elmer). 200ng of total RNA was used as input for SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Clontech). Sequencing was performed on the NextSeq500 instrument (Illumina) using v2 chemistry, resulting in average of 25-30M reads per library with 1x75bp single end setup.