GSM1894009: ChIP-Seq analysis of Paf1 in mouse ESCs under shControl knockdown treatment replicate 3; Mus musculus; ChIP-Seq
Ten million cells were used for the ChIP and precipitated using 2 micrograms of serum-purified antibody against Paf1. Original reference: Zhu B. et al. (2005) "The human PAF complex coordinates transcription with events downstream of RNA synthesis" Libraries were generated as described before, including end-repair, A-tailing, adapter (Illumina Truseq system) ligation and PCR amplification.