RNA sequencing of Passer montanus
Passer montanus (adult workers) were live-trapped using sweep nets. Total RNA was isolated using TRIzol Reagent (Invitrogen, USA) and cDNA was synthesized using the PrimeScript RT reagent kit with gDNA Eraser (TaKaRa, Japan) and a 500bp library constructed. The paired-end library was sequenced (Illumina HiSeq4000) with both directions of 152bp reads.