GSM1524330: 3 Wildtype sibling 3dpf; Danio rerio; RNA-Seq
Embryos for RNA isolation were homogenized using Bullet Blender Homogenizer (Next Advance, Averill Park, NY); subsequently RNA was extracted using the RNeasy Micro Kit (Qiagen) according to the manufacturer’s instructions, and stored at -80°C. The integrity of the RNA was confirmed by Lab-on-chip analysis using the 2100 Bioanalyzer (Agilent Technologies). RNA libraries were prepared for sequencing using standard Illumina protocols