GSM2574886: pool12; Arabidopsis thaliana; RNA-Seq
RNA was isolated using the TRIzol extraction protocol (Life Technologies Copr. Carlsbad, USA). DNA traces were removed with the Ambion DNA-free kit (Life Technologies Copr. Carlsbad, USA) and purified RNA was stored in TE buffer at -80°C. Single-read libraries were prepared with 1mg of total RNA per sample using the Illumina TruSeq RNA Sample Preparation Kit v2 (Illumina Inc. San Diego, USA) based on poly-A RNA purification.