Whole genome of BR19001YR
For PacBio RS sequencing, 8ug of input genomic DNA was used for 20kb library preparation. Total library was prepared using PacBio DNA Template Prep Kit 1.0. SMRTbell templates were annealed using PacBio DNA/Polymerase Binding Kit P6. The PacBio DNA Sequencing Kit 4.0 and 8 SMRT cells were used for sequencing. SMRT cells (Pacific Biosciences) using C4 chemistry and 240 min movies were captured for each SMRT cell usin the PacBio RS II sequencing platform.