GSM2575709: H3RIP_caf1Δdcr1Δ 2; Schizosaccharomyces pombe; RIP-Seq
RNA libraries were prepared with NEBnext Ultra Directional RNA Library Prep Kit for Illumina (NEB); DNA library construction with NEBNext Ultra II DNA Library Prep Kit for Illumina kit (NEB). siRNA library: ligation of 3' adapter (5'-App CTG TAG GCA CCA TCA AT/ddC/-3') and 5' adapter (5'-GUU CAG AGU UCU ACA GUC CGA CGA UC-3'), reverse transcription, PCR of cDNA with Illumina P5 5' primer and barcoded llumina P7 3' primer. Immunoprecipitation of crosslinked (1% formaldehyde, 15 min) cells, decrosslinking, protein degradation and DNA depletion.