GSM2070208: CD5-VM_rep2; Mus musculus; RNA-Seq
RNA was extracted using the Qiagen RNeasy plus kit (Cat# 74134) in accordance with the supplied manual. A modified Kapa Biosystems (Wilmington, MA, USA) KAPA Stranded mRNA-Seq kit for whole transcriptome libraries was used to primarily target all polyA RNA. Briefly, library construction proceeded from isolation of total RNA species, followed by mRNA (poly-A) isolation, 1st and 2nd strand cDNA synthesis, Life Technologies Ion adaptor ligation, amplification, and bead templating.