GSM1520703: 982_BM9; Bos taurus; OTHER
Zymo Quick-gDNA MiniPrep kit; Zymo Genomic DNA Clean and Concentrator Roche Nimblegen Developer Library Kit Hybridisation; Illumina TruSeq v2 kit Our strategy for hybridisation and sequencing was to make one single equimolar pool of all 16 gDNA libraries, each having different adapter barcodes to allow us to de-multiplex sequence data into the correct sample for analysis. We then split the pool in four parts and performed hybridisation captures on each part using 2x capture reagents, resulting in 1x capture for every 2 samples. All captures were then re-pooled and then split into three parts for sequencing on three HiSeq lanes. We used pooling strategies to attempt to reduce variation in hybridisation, and because we were ultimately pooling the captured libraries to sequence over three lanes.