GSM3391421: RNAleaf_rep2; Camellia sinensis var. assamica; RNA-Seq
Total RNA was extracted from young leaf of tea tree following the cetyltrimethylammonium bromide (CTAB) protocol (Doyl and Doyl 1990). Genomic DNA was removed by TURBO DNA-free™ Kit (Ambion) and RNA quality and quantity was assessed, respectively, by Agilent 2100 BioAnalyzer (Agilent Technologies Inc. Santa Clara, CA) and NanoDrop2000c (Thermo Scientific). One microgram of total RNA per sample was utilized for library preparation using the Illumina TruSeq sample preparation kit (v2) with polyA mRNA selection as per manufacturer's instructions (Illumina, San Diego, CA). three libraries were pooled and sequenced using an Illumina HiSeqX-ten with paired end reads of 150 bp at Biomarker technologies company.