Bacteria in soiled sands experiment (OS-73b)
These are amplicon sequence libraries of sequences from 250-500                 bases, single direction read. Pyrotag sequencing of bacterial small subunit                 ribosomal RNAs (SSU rRNAs) and SSU rRNA genes was performed using multiplexed,                 bar-coded primers targeting the V1-V3 region of the bacterial SSU                 rRNA.
Genomic DNA was extracted from Pensacola sands using                     the MoBio Powersoil DNA extraction kit (Mo Bio Laboratories, Carlsbad, CA). RNA                     was extracted from sand samples according to the method described by Chin et al.                     2008 (Microbial Ecology 55:489-499), and reverse transcribed using a general                     bacterial primer targeting the SSU rRNA. gDNA and cDNA were amplified with                     primers 28F (GAG TTT GAT CNT GGC TCA G) and 519R (GTN TTA CNG CGG CKG CTG) by                     the Research and Testing Laboratory (Lubbock, TX, USA). Amplicons were then                     pooled for Roche 454 pyrotag sequencing using Titanium technology, resulting in                     single-direction reads on the order of 250-500                     bases.