GSM4563935: H889 hSCLC NKX2-1 ChIP; Homo sapiens; ChIP-Seq
Cells (~7 x107 cells) in the exponential growth phase were fixed and nuclear extract were prepared. Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with 10 μg antibody. Libraries were constructed by using the NEBNext® ChIP-Seq Library Prep Master Mix (NEB,E6240) and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1, E7335S, Index Primers Set 2, E7500S) with Agencourt AMPure XP magnetic beads (Beckman coulter, A63881) exactly as per manufacturer's instructions.