Sequences of pooled flow sorted rye A chromosomes (1R-7R)
Individual rye chromosomes were isolated by flow sorting and shotgun sequenced by 454 Titanium pyrosequencing
Intact mitotic chromosomes were isolated by                          flow cytometric sorting and the purity of the obtained chromosome                         suspension was determined by FISH essentially as described                          previously (Suchankova et al., 2006). The DNA of sorted                          chromosomes was purified and amplified by MDA as described                          by Simkova et al. (2008).