GSM1893319: LA16P9; Pseudotsuga menziesii; RNA-Seq
Total RNA was extracted using a CTAB based extraction method modified after Chang, 1993. After precipitation, a DNase I digestion was performed using Qiagen's RNase-Free DNase Set (Cat. no. 79254). Afterwards, DNase was removed using silica columns (Qiagen RNeasy MinElute, Cat. no. 74204). RNA integrity was checked on a 2100 Bioanalyzer (Agilent, CA, USA)) using the RNA 6000 nano assay and the Plant total RNA protocol. Sequence library was prepared using the TruSeq RNA Sample Preparation Kit v2 starting with 4ug total RNA.