GSM4225132: Ent4; Homo sapiens; RNA-Seq
RNA was eluted using RNeasy Mini Kit (Qiagen, #74104) according to the manufacture protocol. PolyA fraction (mRNA) was purified from 500ng of total RNA following by fragmentation and the generation of double-stranded cDNA. Then, end repair, A base addition, adapter ligation and PCR amplification steps were performed