GSM2753935: Fbc-2; Homo sapiens; RNA-Seq
Total RNA of fibrocytes and CAFs was isolated using the mirVana miRNA Isolation Kit. Extracted total RNA were treated with the DNA-free Kit. Large 18S and 28S ribosomal RNAs (rRNAs) were removed from total RNA with the RiboMinus Transcriptome Isolation Kit. The rRNA-depleted RNA was precipitated with Pellet Paint (Novagen Inc, Madison, WI) and checked on the Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA). The rRNA depleted RNA was fragmented by incubation for 5 minutes at 94°C in 5× Array Fragmentation Buffer. RNA libraries were prepared for sequencing using standard Illumina protocols