RNA from vesicles of A. laidlawii PG8R10
Acholeplasma laidlawii PG8R10 was treated with ciprofloxacin (2x MIC). The isolation of the A. laidlawii PG8R10 extracellular vesicles was performed on 3 biological replicates. The vesicles were treated by DNAse and RNAse. RNA was extracted with the RNeasy Micro kit (Qiagen). To construct library using the NEBNext Small RNA library prep kit (Illumina). Libraries were sequenced using the HiSeq 2500 system (Illumina).