GSM2411052: CDH1-/THY1+ RP7; Mus musculus; RNA-Seq
RNAs were isolated using RNeasy-plus micro kit (74034, Qiagen). Libraries for each sample were constructed using the Illumina TruSeq Stranded Total RNA Library Prep Kit / Ribo-Zero Gold kit as per manufacturer’s instructions. Libraries were quantified using an Agilent 2200 TapeStation, pooled equimolar and sequenced on an Illumina NextSeq500 instrument using a NextSeq150-v2 Mid Output reagent kit and flowcells.