GSM2332907: N3_CD4; Homo sapiens; RNA-Seq
T cells were FACS-sorted with an Aria III instrument into CD4 and CD8 subsets. RNA was extracted with Qiagen RNeasy mini kit. cDNA was amplified using the Ion Ampliseq Transcriptome Human Gene Expression Core Panel (targeting 20812 RefSeq genes). 16 libraries were generated and barcoded using Ion Xpress Barcode Adapters 1-16 kit, combined together to generate polls of 8 samples, loaded on the Ion Chef instrument for template preparation and loading on Ion P1 v3 chips.