GSM3319617: Help 3 secondary effectors; Mus musculus; RNA-Seq
CD8 T cells were FACS-sorted from the spleens of vaccinated mice based on tetramer+ CD8+ phenotype. RNA was isolated using RLT reagent. Strand-specific libraries were generated using the TruSeq Stranded mRNA sample preparation kit (Illumina) according to the manufacturer's protocol. RNA libraries were prepared for sequencing using standard Illumina protocols