Deep Sequencing of Off Target Site 4 in treated adult 4
Sites of partial homology to the PNA binding site were identified. gDNA was extracted from tissue. Amplicons were then generated and nucleotide changes from the reference genome were analyzed. The primers used for off target site 4 were (forward first): (5-NNN NNN CCCTCTGTGGACTGAGGAAG-3 and 5-NNN NNN TGATGAGCTACGGGTATGTGA-3). Each N is a random base.