GSM1521876: Parent_Micro-Tom; Solanum lycopersicum; miRNA-Seq
Total RNAs of young leaves of Micro-Tom, WVa700, Micro-Tom×WVa700 and WVa700×Micro-Tom were extracted using the Trizol reagent (Invitrogen Inc.) according to the manufacturer’s protocol. The RNAs were sent to the Beijing Genomics Institute (BGI) for sequencing. Small RNAs were enriched and ligated to a 5’ adaptor and a 3’ adaptor sequentially, then converted to cDNA by RT-PCR, and used for Hiseq high-throuput sequencing directly.