RNAseq of Trypanosoma congolense: tsetse mouthparts
RNA from metacyclic parasites was extracted using the RNeasy Kit (Qiagen, UK), yielding a total RNA output of between 48 and 246ng. RNASeq libraries were prepared at Centre of Genomic Research (Liverpool, UK) using the NEBNext® Ultra™ II Directional RNA Library Prep Kit with polyA selection (Poly(A) mRNA Magnetic Isolation Module) (New England Biolabs, UK) as per standard procedure. RNASeq libraries were sequenced on the HiSeq2500 platform (Illumina Inc, USA) as 150 paired ends, producing 280 million mappable reads.