Sequencing of RNAs associated with AtRAP
Rosette leaves of 4-week-old atrap-1/pAtRAP::AtRAP-GFP transgenic plants were applied to RNA-immunoprecipitation. RNAs associated with AtRAP were extracted by TRIzol reagent (Invitrogen) and then treated with DNase (Roche) and subjected to RNA-seq following "NEBNext Ultra Directional RNA Library Prep Kit for Illumina" (NEB).