Whole transcriptome paired-end sequencing of sample 3_CMP
Total RNA from sorted CMP was isolated by Trizol (Ambion) and profiled on 2100 Bioanalyzer (Agilent). About 1 ng total RNA was amplified using the Ovation RNA-Seq System V2 kit (Nugen), and libraries constructed using Ovation Ultralow Library Systems and Ovation Ultralow DR Multiplex System 1–8 (Nugen) according to the manufacturer’s instructions. Transcriptomic sequencing (RNA-Seq) was performed on the Illumina HiSeq platform using the standard paired-end protocol. In total 60-160 million 76 base pair (bp) reads were generated per sample.