Sequencing and de novo transcriptome assembly of the Chinese giant salamander (Andrias davidianus)
The A. davidianus were pooled from 8 tissues (spleen, liver, muscle, kidney, skin, testis, gut and heart) and used for total RNAs library construction. RNA-Seq libraries was generated using the TruSeq RNA-Seq Sample Prep kit according to the manufacturer's protocol (Illumina Inc., San Diego, CA). Poly-A RNAs were isolated from total RNA and chemically fragmented. First and second strand cDNA synthesis were followed by end-repair and adenosines were added to the 3'ends. Adapters were ligated to the cDNA and 200 ± 25 bp fragments were gel puri?ed and enriched by PCR. The libraries were quanti?ed using Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA) and run on the Illumina HiSeq2500 platform (Illumina Inc.). Paired-read sequences,125 nt in length, were collected.