GSM2492414: siPNPase_J2_IP (rep1); Homo sapiens; RNA-Seq
HeLa cell lysate was prepared with NP-40 lysis buffer and cellular dsRNA were pulled down with J2 antibody (anti-dsRNA mAb J2, Prod. Nr. 10010500 from Scicons). RNA was extracted using Trizol reagent for library preparation. Libraries were prepared with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina, v1.0 (cat. no. E7420) according to the manufacturer's guidelines.