GSM2570294: AtGNC_IP_Rep1; Arabidopsis thaliana; ChIP-Seq
ChIPed DNA was prepared from the leaves of 3-week-old myc-tagged overexpression plants using the protocols described in (Gendrel et al., 2005). Briefly, freshly harvested leaves were cross-linked in 1% formaldehyde under vacuum. The chromatin was extracted, sonicated (10% sonicated chromatin was saved as INPUT sample without incubating with anti-myc antibody) and the DNA was immunoprecipitated by anti-myc antibody (Millipore) as the IP sample. Both IP and INPUT DNA were purified and over 15 ng of each DNA from two biological replicates of each genotype were used for library construction Library construction was performed at Clinical Genomics Center, Mount Sinai Hospital, Toronto, Canada.