GSM4184927: P5 Sox9Cre; Vangl2f/f rep1; Mus musculus; RNA-Seq
The left lung lobe from control and mutant mice were homogenized in 1 ml TRIzol(Life Technologies) and 200 μl chloroform was then added. After centrifugation, the upper aqueous layer was removed and mixed with an equal volume of 70% ethanol. RNA was extracted with the RNeasy Mini Kit (Qiagen) following the manufacturer's instructions. RNA quality was evaluated using the Agilent 2100 Bioanalyzer RNA libraries were prepared for sequencing using standard Illumina protocols