GSM3932617: 10x Genomics Fresh GMP; Mus musculus; RNA-Seq
RNA was extracted with 10x Genomics Single Cell Chip Single cell suspension in RT Master Mix is loaded on the Single Cell a Chip and partition with a pool of about 750,000 barcoded gel beads to form nanoliter-scale Gel Beads-In-Emulsions (GEMs). Upon dissolution of the Gel Beads in a GEM, the primers with the unique cell barcodes are released and mixed with cell lysate and Master Mix. Incubation of the GEMs then produces barcoded, full-length cDNA from poly-adenylated mRNA. Full-length, barcoded cDNA is then amplified by PCR to generate sufficient mass for library construction. 10x Genomics