GSM2068581: 200_14; Mus musculus; OTHER
Ligation-Free Ribosome Profiling All tissues were homogenized using a glass dounce homogenizer. Lysates were clarified and one half used for poly(A)+ RNA extraction and the other to generate and isolate ribosome-protected RNA fragments. For RNA-Seq libraries: Standard RNA-Seq library preparation. For ribosome profiling libraries: Ribosome footpring libraries were prepared by ligation-free methods utilizing template switching. Poly-A tailing and PCR. RNA-Seq and Ligation-free Ribosome profiling