GSM2332433: Trpc3lox/lox-2; Mus musculus; RNA-Seq
Bone marrow-derived macrophages, in vitro differentiation to the M1 type and Total RNA was extracted using 5 PRIME PerfectPure™ Purification System (5prime). TruSeq Stranded mRNA Sample Prep Kit (Illumina, #RS-122-2103) was used with 100ng of total RNA for the construction of sequencing libraries RNA libraries were prepared for sequencing using standard Illumina protocols