GSM1894134: IgG_ChIPseq_I; Mus musculus; ChIP-Seq
ChIP experiments using anti-Mef2c (Santa Cruz; sc-13266X; Lot#C2309) or anti-IgG (Santa Cruz; sc-2028; Lot#J2114) antibodies were performed using the EZ-ChIP Kit (Upstate/Millipore) according to the manufacturer’s protocol. Purified DNA from chromatin immunoprecipitations (10-50 ng) was adapter-ligated using the Illumina-compatible NEXTflexTM ChIP Seq-Kit (Bioo Scientific) for DNA inserts ≥70 bps.DNA fragments between 350-550 bp were isolated using PippinPrep (Sage Science) and quantified and sized on a microfluidics-based Bioanalyzer 2100 using a High Sensitivity DNA Kit (Agilent).