Environmental DNA (eDNA) water sample collected from central Australia targeting vertebrates
Appropriate dilutions of DNA extracts were qPCR (quantitative PCR) amplified in duplicate targeting a 90-110 bp region of the 12S rRNA mitochondrial gene. Pre- and post-PCR work was spatially separated to ensure no/minimal contamination from previously amplified DNA into samples pre-PCR. Unique MID tag combinations were used for all samples. Full details provided in "Identifying error and accurately interpreting eDNA metabarcoding results: A case study to detect vertebrates at arid zone waterholes" published in Molecular Ecology Resources