GSM3615067: control_ATACseq_rep2; Mus musculus; ATAC-seq
For ChIP-seq, tissue samples were fixed in 1% formaldehyde for 10 minutes, nuclei were extracted and lysed, and chromatin was sheared to ~300 bp average length. Immunoprecipitations were performed using antibodies to H3K27ac (Abcam AB4729) or H3K27me3 (Millipore 07-449), coupled to Dynal Protein A dynabeads (Invitrogen). For ATAC-seq, tagmentation was performed using the Nextera DNA Libarary Preparation kit (Illumina). 12.5 microlitres of Tn5 enzyme was incubated with the cells for 30 minutes at 37 degrees. Libraries were prepared according to the Illumina protocol. Illumina HiSeq 2500 (paired ends)