RNA-Seq of Sus scrofa:  infant descending colon exfoliome
Using 100 ng of total RNA in NuGen's Ovation 3' DGE Sytem following manufacturer's instructions.  The cDNA from this step was fragmented using the Covaris then 800 - 1000 ng of this fragmented cDNA was used in the Encore Rapid Library System kit from NuGen.  The final library was prepped following the manufacturer's instructions.