GSM4432426: CXCR4+ / ITGβ1+_CB_CD34+ RNA-seq; Homo sapiens; RNA-Seq
Cultured samples were directly lysis into RLT plus buffer and total RNA were extracted using RNeasy plus Micro Kit (Qiagen) CXCR4+ / ITGβ1+/ DAPI- isolated through FACS sorting from 16 weeks organoids into RLT buffet supplemented with 10uM b-mercaptoethanol and RNA was harvested using the RNAeasy Micro Kit from Qiagen. Whole transcriptome Ovation Solo RNA seq Library Preparation Kit (TECAN, NuGEN) was used for the library preparation. Cultured samples were directly lysis into RLT plus buffer and total RNA were extracted using RNeasy plus Micro Kit (Qiagen) TruSeq Stranded Total RNA kit Gold (Illumina) or Whole transcriptome Ovation Solo RNA seq Library Preparation Kit (TECAN, NuGEN) was used for the library preparation of all samples