siRNA from the sweet potato leaf phytobiome found in three cultivars in Barbados
Total RNA was extracted from two infected leaf samples of the sweet potato cultivar CBS32 using TRIZOL and subjected to Next-Generation DNA Sequencing (NGS) using Ilumina technology which was carried out by Fasteris Inc. Genome processing consisted of separation of RNA by 3.5% agarose gel electrophoresis and isolation of RNA bands <30 nucleotides (nts), followed by ligation with single stranded 3’ and 5’ adapters. These ligated fragments were then reverse transcribed and amplified by PCR to generate a library of short inserts.