GSM1895155: BAP1 KO GMP 2; Mus musculus; RNA-Seq
Bone marrow was extracted and red cell lysed, cells were stained for GMP markers (Lineage-c-Kit+Sca1-CD34+Fcϒ), and sorted using a FACS Aria. RNA was harvested using Trizol reagent. Smart Clontech RNA Kit (Cat# 635001) was used with 20 ng RNA for library construction. 10ng of high quality total RNA was amplified using the SMARTer Universal Low Input RNA Kit for Sequencing (Clontech Laboratory, cat# 634940) according to manufacturer instructions. Amplified material underwent whole transcriptome library preparation according to the Ion Total RNA-Seq Kit v2 protocol (Life Technologies), with 16 cycles of PCR.