GSM2332450: SEM_2umEPZ5676_Rep2; Homo sapiens; RNA-Seq
Nascent RNA-seq: 108 4sU-treated cells were lysed in 10ml Trizol. RNA was extracted using chloroform and precipitated with EtOH. 4sU RNA was biotinylated with 1mg/ml Biotin-HPDP and nascent RNA was enriched for using streptavidin-coated beads. Libraries were prepared according to Illumina's protocol accompanying the Ultra RNA Library Prep Master Mix Set (Part# E7530).