GSM3391410: ZNFWT_LIF_1; Mus musculus; RNA-Seq
RNA was purified using Trizol reagent (Life Technologies) according to the manufacturer´s instructions. For RNA-sequencing analysis, trizol-purified RNA was further cleaned with the RNeasy mini kit (Qiagen) according to the manufacturer´s instructions. Libraries were prepared from 1ug of purified total RNA using the TruSeq stranded mRNA sample preparation kit (Illumina) and quantified with a Qubit fluorometer (Thermo). Uniquely indexed libraries were pooled in equimolar ratios and sequenced on an Illumina NextSeq500 with single-end 75 bp. Library preparation and sequencing were performed by the Deep Sequencing Facility of the Max-Planck Institute of Immunobiology and Epigenetics.