Salmonella enterica subsp. enterica serovar Berta ATCC 8392 Nalidixic acid-resistant mutant
Isolates were cultured from single colonies in brain heart infusion broth (Oxoid) for 3-4 hrs at 37°C, and genomic DNA was extracted from broth cultures using the Maxwell® 16 Cell SEV DNA Purification kit (Promega, Madison, WI). DNA was quantified using the Quant-iT High-Sensitivity DNA Assay Kit (Life Technologies Inc., Burlington, ON). Sequencing libraries were constructed from 1 ng of gDNA using the Nextera XT DNA sample preparation kit (Illumina, Inc., San Diego, CA) and the Nextera XT index kit (Illumina, Inc.).