GSM2333121: lin-61(tm2649)_YA-rep1; Caenorhabditis elegans; RNA-Seq
Total RNA was extracted in TriPure Reagent (Roche), treated with baseline zero DNase (Epicentre), and purified with Zymo Research RNA Clean and Concentrator-5 (Cambridge Bioscience), all according to the manufacturers' instructions. Ribosomal RNA was depleted using the Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat) (Illumina). Ribosomal RNA-depleted RNA was used to make strand-specific libraries using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions.