RNA-seq of Pseudocrossidium replicatum protonema: 10 d old Control, 10 min of fast dehydration at 23% RH
Total RNA was extracted with Trizol from 10 days old monosporic protonemal tissue, which was dehydrated by exposure to 23% RH (-198 MPa) for 10 minutes (T1). Equal amounts of total RNA obtained from three independent biological replicates were pooled. Library was prepared following the instructions of the TruSeq RNA Sample Preparation Kit.