GSM3934410: Gbm2_Etoposide_0.3uM; Homo sapiens; RNA-Seq
Total RNA was isolated from GBM2 cells using the RNeasy Kit (Qiagen), 0.25 ug total RNA was used to isolate mRNAs and for library preparation. PolyA RNA was isolated using the NEBNext® Poly(A) mRNA Magnetic Isolation Module. Barcoded libraries were made using the NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich MA). Libraries were pooled and single-end sequenced (1X75) on the Illumina NextSeq 500 using the High-Output V2 kit (Illumina).