HEK293_RBPMS_overexpression_replicate_1
HEK293 inducible FLAG-HA-tagged RBPMS cells were cultured for 72 hours in the presence of doxycyline (0.01 micrograms per ml) and represent RBPMS over-expresion. The experiment was conducted as a biological replicate (this being replicate 1). Total RNA was extracted using TRIZOL (Invitrogen) according to the manufacturer’s instructions. Poly(A) RNAseq libraries were constructed per manufacturer instructions by the Rockefeller University Genome Center and sequenced using the Illumina HiSeq 2500 platform.