sRNAs of Araucaria engustifolia
Total RNA was extracted from Brazilian pine fresh leaves. RNA sequencing was performed using the Illumina HiSeq2000 platform, one sRNA library was constructed and sequenced, comprising 28,376,092 single-end reads with a length of 50 bases. The sRNA library building follows a series of standard steps, briefly described as follows: gel purification of the RNA corresponding to fragments ranging from 20-30 nt, ligation of the 3p and 5p adapters separately and followed by gel purification each, cDNA synthesis and cDNA gel purification, and, finally, PCR amplification to generate a cDNA colony template library for deep sequencing.