GSM1525105: p-KDM3A_HS(+); Homo sapiens; ChIP-Seq
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with KDM3A or p-KDM3A antibody.The sonicated DNA fragments were purified as Input control. The DNA fragments were end-repaired, adenylated,ligated to adaptors and PCR-amplified for 18 cycles. The PCR products corresponding to bp 250-450 were gel-purified, quantified and stored at -80°C until use for sequencing