GSM1524385: T29_MB-seq_rep1; Homo sapiens; OTHER
Genomic DNA were extracted from T29 and T29H cell lines by Qiagen DNeasy Blood & Tissue Kit (Qiagen, Germany) and 20 mg/mL RNase (Qiagen, Germany) was used to degrade contaminated RNA in DNA samples. DNA integrity was verified by agarose gel electrophoresis. Quantity of DNA was measured using Quant-iT PicoGreen dsDNA Kits on Qubit 2.0 (Invitrogen, USA) Genomic DNA from T29 cell line with the input amount of 1 μg, 500 ng, 200 ng, 50 ng, respectively, was fragmented to ~100-500 bp using Covaris E210 sonicator (Covaris Inc. USA). Fragmented DNA was end-repaired, A-tailed, and ligated to C-hydroxylmethylated Illumina Multiplexing adapters following the standard Illumina protocol.MB-seq library construction for genomic DNA from T29H exactly followed the protocol mentioned above.