GSM1406464: IR; Brassica napus; miRNA-Seq
Roots were cleaned and freezed by liquid N2 and total RNA were isolated by Trizol reagent (Invitrogen).Total RNA from 6dpi-old roots was mixed for sequencing. In total, two samples, untreated root (UR) and V. longisporum infected root (IR) were used to construct small RNA libraries. small RNA libraries were prepared for sequencing using standard Illumina protocols