Rattus norvegicus Transcriptome or Gene expression D42
20 μg of total RNA equivalently pooling from 5 SD rats of at day 42 (D42) was isolated by polyacrylamide gel electrophoresis (PAGE). Small RNA molecules (less than 30 nt) were purified from the gel and ligated with Solexa adapters to their 5' and 3' termini, and then they were used as the templates for cDNA synthesis. cDNA was amplified by using the adaptor primers with 8 PCR cycles to produce sequencing libraries and fragments of approximate 92 bp were isolated by agarose gel. The purified DNA was used directly for cluster generation and sequence analysis by using the Illumina Genome Analyzer (Illumina, San Diego, CA, USA). The image files generated by the sequencer were then processed to produce digital-quality data. After adaptor sequences were masked and contaminated reads were removed, clean reads were processed for computational analysis.