HEK293_RBPMS_baseline_replicate_2
HEK293 inducible FLAG-HA-tagged RBPMS cells were cultured for 72 hours in the absence of doxycyline and represent baseline RBPMS expresion. The experiment was conducted as a biological replicate (this being replicate 2). Total RNA was extracted using TRIZOL (Invitrogen) according to the manufacturer’s instructions. Poly(A) RNAseq libraries were constructed per manufacturer instructions by the Rockefeller University Genome Center and sequenced using the Illumina HiSeq 2500 platform.