GSM4564599: K904-3; Serratia marcescens; RNA-Seq
RNA was isolated using the RNeasy Mini Kit (Qiagen), treated twice with DNase I, and concentrated with a spin concentrator (Monarch, New England Biolabs). Libraries were made by GeneWiz, Steps include: ribosomal RNA depletion, RNA fragmentation and random priming, first and second strand cDNA synthesis, end repair, 5' phosphorylation and dA-tailing, adaptor ligation, PCR enrichment, and sequencing.