GSM4431925: WT_Rep1; Homo sapiens; RNA-Seq
Total RNA was extracted and purified using the RNA Clean & Concentrator kit (Zymo Research, R1013) according the manufacturer's protocol. On-column DNAse I treatment was performed to eliminate genomic DNA contamination. RNA-seq libraries were prepared using the TruSeq Stranded mRNA library prep kit (Illumina, RS-122-2101) and analyzed by paired-end poly(A) RNA sequencing with an illumina HiSeq system.