GSM1897287: CAMA1; Homo sapiens; RNA-Seq
extract protocol RNA (1 ug) from each sample was reverse transcribed into cDNA by using the Illumina TruSeq Stranded mRNA kit. Libraries were sized on an Agilent Bioanalyzer, and their concentrations were validated by qPCR. Six different libraries were normalized to 10nM and pooled, 13pM of pooled libraries were loaded onto an Illumina cBot for cluster generation, and the flow cell was subjected to 50-cycles of paired-end sequencing on an Illumina HighSeq 2000.