GSM2571844: Mino_DLL1-IgG-gsi-gsi_RNA-Seq_r3; Homo sapiens; RNA-Seq
RNA-Seq: Cells were pelleted and flash-frozen in liquid nitrogen. RNA was extracted from pellets via Qiagen QIAshredder and RNeasy columns. RNA-Seq: Strand-specific RNA-Seq libraries were generated as follows: Oligo dT beads were used to isolate the poly-adenylated fraction of total RNA, followed by zinc-mediated fragmentation and DNase treatment. A 3' single-stranded RNA adaptor was ligated to RNA fragments, and an adaptor-seated primer used for first-strand cDNA synthesis. RNA was then hydrolyzed, and a 3' DNA adaptor was ligated to ssDNA fragments, which were then PCR enriched with barcoded, adaptor-seated Illumina PCR primers. Final libraries were isolated by SPRI purification (forward SPRI x2), and paired-end sequenced on Illumina NextSeq (38 bp x2).