GSM4185523: P7 unfractionated Rep2; Mus musculus; RNA-Seq
Testis tissue was dissociated with two step enzymatic digestion with collagenase-IV and trypsin-EDTA. Single cell suspension was direclty used or washed and enriched for ITGA6 expressing cells. Cells were diluted for loading on Chromium chip for 10X Genomics library preparation and subsequent sequencing. Chromium Single Cell Kit [v2 chemistry] 10x single cell RNA sequencing