GSM4188936: PolyQ on NF (PQ_NF); Drosophila melanogaster; RNA-Seq
RNA was isolated from 30 pairs of eye discs dissected out from wandering third instar larvae in PBS  using Trizol (Invitrogen, USA) reagent as per manufacture's protocol. 1µg of the isolated RNA was subjected to DNAase treatment using 2U of TurboTM DNase (Ambion, Applied Biosystem) enzyme for 30 min at 37°C. The reaction was stopped using 15mM EDTA followed by incubation at 65°C for 5-7 min and purification on RNAeasy column (Qiagen). The purified RNA samples were processed for preparations of cDNA libraries using the TruSeq Stranded Total RNA Ribo-Zero H/M/R (Illumina) and sequenced on HiSeq-2500 platform (Illumina) using 50bp pair-end reads. RNA libraries were prepared for sequencing using standard Illumina protocols