GSM3389727: BSC_1711: Control_3; Homo sapiens; Bisulfite-Seq
genomic DNA extracted and purified using the Zymo Duet Kit DNA was extracted from BA9 cortex from RTT and Control samples using the Zymo Duet Kit (Zymo). 200ng of total DNA was bisulfite converting using the Zymo Lighting kit (Zymo) and 50ng of converted DNA was used as input for library construction using the Illumina TruSeq DNA Methylation Kit (Illumina).  Each sample was given a unique barcode and 14 cycles of PCR amplification.  Final libraries were size selected with two rounds of KAPA Pure Beads: 0.7X Left and 0.65X Left/0.55X Right for a final library size centered around 370-470 base pairs. Final libraries were assessed with Bioanalyzer (Agilent), quantified, pooled and run for 150 bp paired end sequencing on two lanes of the HiSeqX (Illumina). Bisulfite-Seq 150 bp paired end on Illumina HiSeqX