GSM2333046: 9YD; Mus musculus; miRNA-Seq
Ovaries were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. RNA was extracted according to Matkovich & Dorn (2015; Gary R. Skuse and Maureen C. Ferran (eds.), Cardiomyocytes: Methods and Protocols, Methods in Molecular Biology, vol. 1299, DOI 10.1007/978-1-4939-2572-8_3) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols