GSM4502656: BST_12h_mRNAseq_rep2; Mus musculus; RNA-Seq
Organs (OB, BRN, CBL, BST, STM, LIV, and iBAT) were homogenized in Lysis RLT Buffer (Qiagen) supplemented with 1% β-mercaptoethanol (Sigma-Aldrich) using the OMNI tissue homogenizer (TH) (OMNI International). Homogenized organ lysates were then loaded onto the QIAshredder homogenizer spin columns (Qiagen) for further homogenization and elimination of insoluble debris. Total RNA was extracted using RNeasy Mini Kit (Qiagen), according to the manufacturer's protocol. White-adipose tissues (pgWAT and psWAT) were homogenized in Qiazol (Qiagen) and RNA extracted with the Lipid RNeasy Lipid Tissue Mini Kit (Qiagen). mRNA was prepared for sequencing using the TruSeq stranded mRNA sample preparation kit (Illumina), with a selected insert size of 120–210 bp mRNA was prepared for sequencing using the TruSeq stranded mRNA sample preparation kit (Illumina), with a selected insert size of 120–210 bp