GSM4565532: ESC_ATRXWT_GFP; Mus musculus; ChIP-Seq
Crosslink ChIP was performed as described (Law et al. 2010; Truch et al. 2018). Briefly, cell were harvested and crosslinked in 2mM EGS for 45min and 1% PFA for 10min. Chromatin was then sheared to 150-500 bp. Chromatin was isolated and used for ChIP-seq according to standard procedures. Libraries were constructed according to the Illumina Tru-seq protocol.