GSM3932035: CONV; Mus musculus; RNA-Seq
Lungs were resected and single cell suspensions were prepared by enzymatic dissociation and sequential filtering (100 and 40 µm) before red blood cell lysis and cell counting. Single-cell 3'-RNA-Seq samples were prepared using single cell V2 reagent kit and Chromium controller (10x Genomics, PN-120237). cDNA quality control was assessed by capillary electrophoresis (Bioanalyzer, Agilent). Single-cell RNA-Seq libraries were prepared using Chromium Single Cell 3' v2 Reagent Kit (10X Genomics) according to manufacturer's protocol. Read 1 (26 cycles) corresponds to cell barcode and UMI, Read 2 (98 cycles) corresponds to 3'-end of RNA molecule. Index 1 (8 cycles) is single cell library barcode.