Neurospora crassa conidiation time course mycelium at time 24
RNA was isolated from mycelium grown in Vogel's minimal medium culture grown at 25 degrees C was harvested on filter paper using a Buchner funnel.  The mycelial pad on filter paper was placed on Vogel's minimal agar medium for 24 hours. At that time, The aerial hyphae readily released free conidia. Few conidial chains were visible under the microscope due to disarticulation of the conidial chains during preparation for microscopy. This mycelium was removed with forceps and frozen immediately in liquid nitrogen for RNA extractoin.  RNA was purified and cDNA constructed using the Illumina mRNA seq prep kit and sequenced on a GA II sequencer.