Illumina HiSeq
Six pooled males and females captured at 8pm and 2am were extracted using the Quiagen universal RNA mini kit. This RNA was sent to the University of Rochester (Rochester, NY) for sequencing and library preparation. The samples were prepped with a TruSeq kit and then sequenced on 1 lane of an Illumina HiSeq 2500 as a High Output v4 125 bp PE project. Data came from the sequencing facility post trimming. They had used Trimmomatic (v.0.32) to eliminate adapter sequences (2:30:10) and removed low quality scores using a sliding window (4:20), removing both trailing and leading sequences (13) and leaving only sequences with a minimum length of 25 for downstream use.