GSM1893950: GRO-Seq analysis in mouse ESCs under flavopiridol treatment replicate 3; Mus musculus; OTHER
Trizol reagent extraction (Invitrogen) BrUTP labeled nascent RNA was used for library preparation. cDNA preparation and strand-specific library construction was performed using the dUTP method. Libraries were sequenced on the Illumina HiSeq 2000 using 50bp single-read method.