whole exome-seq of homo sapiens: adult female blood
-TruSeq DNA Sample Prep: Fragmented 1 ug of g DNA is ligated to 'A' at the 3' end. Illumina adapters are then ligated to the fragments, and smaple is selected by size for 350-400 base pair. The selected samples are amplified by PCR. -Hybridization (twice): The pooled DNA libraries are mixed with capture probed of targeted regions. -PCR Amplification: PCR is used in order to amplify the enriched DNA library for sequencing. Sequencing: A flow cell containing millions of unique clusters is loaded into the HiSeq 2000 for automated cycles of extension and imaging.