GSM4185026: F1 FeCl3 rep1 GW3; Paracentrotus lividus; RNA-Seq
Embryos (about 6000) were harvested 24h p.f., briefly centrifuged and the pellet was suspended in 200 µl Thioglycerol/homogeneization solution. RNA was extracted with Maxwell 16 LEV simply RNA kit (Promega) in Maxwell 16 instrument. Purified total RNA were collected in 50 µL RNase-free water and kept afterwards at -80°C. RNA integrity (RIN>9) was estimated with a Bioanalyzer. RNA libraries were prepared for sequencing by Genewiz LLC (www.genewiz.com) using standard Illumina protocols and via poly A selection