GSM2486592: Fra2_4; Mus musculus; RNA-Seq
Total RNA was extracted from lysates using RNeasy Plus Micro kits (Qiagen) according to the manufacturer’s instructions. mRNA was isolated from 50 ng total RNA using Dynabeads mRNA Purification Kit (Ambion). Ovation® RNA-Seq System V2 (NuGEN Technologies, San Carlos, CA) was used for cDNA amplification from selected mRNA cDNA libraries were prepared using Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA).