GSM1524129: M; Bursaphelenchus xylophilus; miRNA-Seq
Nematode RNAs were collected using the mirVana miRNA Isolation kit (Applied Biosystems, Austin, TX, USA) and the total RNA enrichment procedure was followed. Agilent Technologies 2100 Bio-analyzer (Agilent Technologies, Inc) was used to assess RNA quality, only utilizing samples with RIN (RNA integrity number) greater than eight in downstream experiments. Four small RNA libraries were constructed according to the standard Illumina Sample Preparation Protocol (Illumina, San Diego, CA, USA).