GSM2332925: 2xlink IgG ChIP-seq rep 3, 3-19-14; Drosophila melanogaster; ChIP-Seq
Cells were sonicated and cleared of cellular debris.  Lysates were immunoprecipitated with the corresponding antibodies Briefly, DNA ends were repaired with T4 DNA polymerase, Klenow Large Fragment DNA polyermerase and T4 polynclease kinase.  DNA was purified with PEG bead slurry.  Illumina short sequencing adaptor was ligated to DNA overnight.  Library was purified with PEG beads