DNA-seq of Chrysomya rufifacies: male
Pooled genomic DNA was extracted from the heads of 5 male-producing females (arrhenogenic), 5 female-producing females (thelygenic ) and 5 male flies originating from a lab colony of C.rufifacies using the DNeasy Blood and Tissue DNA Extraction kit following manufacturers instructions (Qiagen Inc., Valencia, CA, USA). A total of 1 g of genomic DNA was sent to a facility for library preparation. Libraries (N = 3) were constructed following TruSeq DNA Sample Preparation Guide by Illumina (Catalog #PE-940-2001. Part # 15005180 Rev. A, November 2010). Sequencing was performed on the 3 paired-end libraries using the Illumina HiSeq2000 sequencing platform (Illumina Inc, SanDiego, CA, USA), read length of 2 x 100 bp. Both of the library preparation and sequencing was completed by the Purdue University Genomics Core Facility (West Lafayette, IN, USA).