IgH repertoire of naive, germinal centre, and memory B cells from SLC-knockout mice
Spleen cells were collected from pools of six SLC-/- mice, by sorting GC (B220+GL7+CD95+) B cells, naïve (B220+CD93-CD43-CD80-PD-L2-CD73-) and memory (B220+CD93-CD43-CD80+PD-L2+CD73+) B cells. RNA was isolated using RNeasy kit (QIAGEN) and cDNA synthesis was performed according to the SuperScript II RT protocol (Invitrogen). Igμ and Igγ transcripts were amplified independently.