GSM3934782: disease rep20; Homo sapiens; MBD-Seq
Endometrial tissue was lysed using Qiagen ATL buffer supplemented with Proteinase K at 55C overnight. DNA was extracted using the Qiagen DNAeasy Blood and Tissue kit To produce methyl enriched DNA fragments, sonicated DNA was subjected to the Methylated-CpG Island Recovery Assay (MethylCollector Ultra – Active Motif). Enriched methyl DNA was then subjected to library construction using the NEBNext DNA library prep kit for Illumina (New England Biolabs)