small RNA-seq of Chinese flowering cabbage: YQ roots for 0 cadmium treated hours
Total RNA was extracted by TRI Reagent (Molecular Research Center, Inc.) according to manufacturer's protocol. Small RNA libraries were constructed from 20 microgram of total RNA using NEB Next Multiplex Small RNA Library PrepSet for Illumina(E7300). RNA-seq libraries were prepared and sequenced by Beijing Novogene Co., Ltd.. PARE libraries were constructed according to the protocols of Zhai et al., 2014.