WGS of Saccharomyces cerevisiae: iSeq F0 parental BC library strain
Genomic DNA was isolated from an overnight culture started from a single colony using the YeaStar Genomic DNA Kit (Zymo Research). Sequencing libraries were generated using a modified Nextera library prep protocol in 96-well format (PMID: 24970088). Raw fastq files were processed by trimming for adaptor sequence and quality using cutadapt 1.7.1 and mapping to the S288C reference genome (R64-2-1) using BWA version 0.7.10-r789. Resulting SAM files were sorted, duplicates were marked and removed, reads were realigned around indels using Picard and GATK. Variants were called using GATK's Unified Genotyper v.3.3.0 and CNV-Seq, and confirmed via visual inspection using samtools.