GSM4498613: T-cell_RNA_seq_rep1; Homo sapiens; RNA-Seq
RNA extraction was performed using the RNeasy Mini Kit (Qiagen). RNA quality was checked by Agilent High Sensitivity RNA ScreenTape, and the two samples with the highest RNA Integrity Number equivalent were chosen (RINe = 9.9). RNA libraries were prepared using Illumina TruSeq Stranded Total RNA Library Prep, including ribosomal RNA removal using Ribo-Zero. Libraries were sequenced with 75-bp paired-end reads using Illumina NovaSeq 6000.