GSM2070585: TCam-2 Romidepsin 16h; Homo sapiens; ChIP-Seq
For chromatin-immunoprecipitation-followed-by-sequencing (ChIP-seq) analysis, cells of interest were fixed by addition of 1/10 Formaldehyde solution (11 % Formaldehyde, 0.1 M NaCl, 1 mM EDTA (pH 8), 50 mM HEPES (pH7.9)) to the culture media followed by 15 minutes of incubation at room temperature. Fixation was stoped by addition of 1/20 volume Glycine solution (2.5 M) and incubation for 5 minutes at room temperature. Cells were scraped and washed once in PBS-Igepal (0.5%) solution and once more in PBS-Igepal-PMSF (1mM) solution. Cells were pelleted by centrifugation and snap frozen on dry ice and stored at -80°C.