GSM2574180: 1496JT; Homo sapiens; miRNA-Seq
Peripheral blood samples from  male and female (44-92 years) were collected into EDTA Tubes- Plasma (Cat# 367863) were inverted five times, stored on ice and processed within 30 minutes of blood draw. The blood samples were centrifuged at 1600 x g for 15 minutes at 4°C and the plasma was isolated. Exosomal RNA was isolated using exoRNeasy Serum/Plasma Maxi kit (Cat# 77064, Qiagen) according to the manufacturer’s instructions with the final elution volume of 12 ul. Sequencing libraries were constructed using ~2ng of total exosomal RNA with the library preparation kit Ion Total RNA-Seq kit v2 (Thermo Fisher, Cat# 4475936)