GSM4188558: RNA-seq, Mirg(Delta/+) ESC Replicate A; Mus musculus; RNA-Seq
Total RNA was extracted using TRIzol. rRNA-depleted RNA-seq libraries were prepared using KAPA RNA HyperPrep Kit with RiboErase (HMR) (Kapa Biosystems). rRNA-depleted RNA-sequencing libraries were prepared from 400 ng DNase-treated RNA using KAPA RNA HyperPrep Kit with RiboErase (HMR) (Kapa Biosystems) using 9 cycles of PCR amplification. Quality control on final libraries was assessed with a fragment analyzer and qPCR for colony forming units prior to pooling and loading on an Illumina FlowCell (NextSeq 500, 75 bp PE reads).