Trichoderma harzianum P49P11 Genome sequence
Fungal spores were inoculated in complete growth media for 48h. Micelia were collected by filtration in vacuum and immediately frozen in liquid nitrogen, and macerated with mortar and pestle. DNA extraction was carried out with the Phenol:Chloroform method. NGS library was prepared with the paired-end Nextera chemistry and sequenced on a HiSeq2500