GSM2411732: LY reactivated SCGs + IFNb, replicate 2; Rattus norvegicus; RNA-Seq
total RNA was extracted using Trizol reagent, precipitated with ethanol, DNase-treated, phenol-chloroform extracted, precipitated once more with ethanol and yields determined by absorption spectroscopy using a NanoDrop (NanoDrop Products). RNA-seq libraries were generated following Illumina TruSeq protocols to remove rRNA using Ribo-Zero, fragmentation, and adaptor ligation.