RNA-Seq of Citrus sinensis leaves
The resulting mRNA was then fragmented into short fragments (200 nt) and used to the first-strand cDNA synthesis with random hexamer-primers. Subsequently, second strand cDNA was synthesized by using DNA polymerase I and purified by AMPure XP beads. Purified second-strand cDNA were proceeded to end repair, 3' end adenosine tailing, sequencing adaptor connection, fragment selection by AMPure XP beads and finally PCR amplification. The library quality was assessed using the Agilent Bioanalyzer 2100 system. After cluster generation, the library amplicons were sequenced on the Illumina HiSeq 4000 platform (Illumina Inc., San Diego, CA, USA) using the paired-end technology in a single run. Illumina GA Pipeline  was used to perform the original image process to sequences, base calling and quality value calculation, in which 125 bp/150 bp paired-end reads were generated.