GSM2333459: PC-3 + rtTA2 + tdTom + Dox #1; Homo sapiens; RNA-Seq
RNA was extracted from cells using the Macherey-Nagel NucleoSpin RNA kit (Ref # 740955). RNA-Seq was performed using the NuGen Ovation Encore Complete kit.  Briefly, 100 ng of RNA was used to generate cDNA and a strand-specific library following the manufacturer’s protocol (NuGEN Technologies, Inc., San Carlos, CA).  ).  Quality control steps including analysis on the BioAnalyzer RNA chip and quantitative RT-PCR for library quantification were performed.  The libraries were then sequenced on the Illumina NextSeq 500 sequencer with a 75-base paired-end run in order to generate 40-50 million read pairs per sample.