GSM2572245: cortex TAB-seq rep1; Mus musculus; Bisulfite-Seq
The tissues for the technical replicates are from independent mouse, and at least two replicates are included in different assay as indicated. Genomic DNA (Qiagen, Cat#: 51306) were extracted according to the manufacturers’ instructions. The sequencing libraries were constructed as described with minor modification. Briefly, genomic DNA with spike-in controls was glycosylated and oxidized using the kit from Wisegene (Cat#: K001). Then, the DNA was further directed to bisulfite conversion using the EZ DNA methylation Gold kit (Zymo Research) according to the instruction manual. The library was sequenced using Illumina HiSeq X Ten. Paired reads were mapped uniquely to the reference genome (mm10, UCSC) by Bismark. Efficient conversion of unmodified cytosine to uracil and efficient conversion of 5mC to 5caU/U were calculated by spiked M.SssI-treated lambda DNA.