GSM4498760: 11_S25_IPS_macrophages_m1_GMCSF_INFG; Homo sapiens; RNA-Seq
After the polarization period, the cells were lyzed and RNA was extracted using RNeasy Mini Kit from Qiagen. RNA content and purity were determined using Nanodrop. RNA integrity was assessed using Agilent Bioanalyzer. RNA sequencing was performed using the HiSeq4000 sequencing system. mRNA sequencing libraries were prepared using the TruSeq Stranded mRNA library prep kit (Illumina) according to manufacturer's instructions.