GSM4073102: Pea3_Rep3; Mus musculus; RNA-Seq
After tissue harvest, RNA was extracted using Qiagen kit. Conversion to cDNA as well as amplification was performed by Clontech SMART-seq v4 Ultra low input RNA kit, as well as cDNA library construction was performed using Nextera XT DNA library preparation kit by core facility at Columbia university prior to RNA sequencing. After preparation of cDNA library, each sample was sequenced using Illumina platform.