GSM2570525: Jurkat shARID5B RNA-seq #2; Homo sapiens; RNA-Seq
RNA was extracted using an miRNeasy kit (Qiagen) with some modifications.  The cell numbers for each sample were counted before cell pellet was collected. ERCC RNA Spike-in mix (1:10 dilution) wasthen  added to each sample according to the cell numbers (Example: 1ul was added for 1 million cells)  after the addition of Qiazol.  After the addition of chloroform, the samples were transferred to a Maxtract high-density column (Qiagen) to be spun down.  The aqueous layer was then retained for downstream RNA extraction following the manufacturer’s instructions.  The extracted RNA was subjected to DNase treatment (Turbo DNA-freeTM kit, Ambion) according to the manufacturer’s instructions to eliminate any genomic DNA present in the samples and then cleaned up using an RNeasy kit (Qiagen). Strand-specific library construction and sequencing of paired-end, 100-bp-long reads by the Illumina Hiseq4000 were performed at the BGI Biotech Solutions (Hong Kong) Co Ltd (Hong Kong).