GSM2576186: T55E; Homo sapiens; RNA-Seq
RNA was extracted using Trizol reagent. NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) was used to purify mRNA from 1μg total RNA. NEBNext® mRNA Library Prep Reagent Set for Illumina (NEB #E6100) was used with 250ng mRNA for construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols.