Illumina ITS  amplicon sequence from sawdust
The internal transcribed spacer (ITS) region was amplified using the fungal specific primers ITS-1F and ITS2.  All amplified PCR samples were cleaned and normalized using the Invitrogen Sequalprep PCR Cleanup and Normalization Kit. DNA library was sequenced using an Illumina MiSeq Sequencer with the 2 300 bp paired end platform using the Illunima MiSeq Reagent Kit v3.