GSM2492182: K1246R18_2_ribominus; Saccharomyces cerevisiae; RNA-Seq
RNA was extracted as previously described (Tollervey, 1987). The RNA samples were either enriched for mRNA using NEBNext Oligo d(T)25 (New England Biolabs) or depleted of rRNA using RiboMinus (Life Technologies) according to the manufacturer’s instructions, producing poly(A)+ or total RNA libraries, respectively High throughput sequencing libraries were prepared using NEBNext Ultra Directional RNA Library Prep Kit for Illumina according to the manufacturer’s instructions.