GSM4502484: Chicken_heart_spatial_RNAseq_D10; Gallus gallus; RNA-Seq
Whole hearts were isolated using aseptic technique and placed in ice cold sterile Hank's Balanced Salt Solution and then blood was carefully removed by perfusing the hearts through the apex. Fresh tissues were immediately embedded in Optimal Cutting Compound (OCT) media and frozen in liquid-nitrogen-cooled isopentane bath, cut into 10µm sections using Thermo Scientific™ CryoStar NX50 cryostat, mounted on 10x Visium slides (pre-cooled to -20°C), and used for library construction. 10x Genomics Visium Spatial Gene Expression 3' Library Construction V1 Kit