RNA-seq Brc9_5
Nine mouse embryos were collected from three independent pregant dams. Total RNA was extracted from mouse embryo brain vesicle tissues using the Trizol method. The cDNA library was constructed using a TruSeq RNA Sample Preparation Kit (RS-122-2101, Illumina) according to the manufacturer’s protocols. The sequencing data was obtained using Illumina HiSeqTM 2000.