CCMP2561_NRKO: KO_N-free_B
RNA was extracted using Trizol Reagent (Thermo Fisher Scientific), genomic DNA was removed with DNase I (TURBO DNA-free Kit, ThermoFisher Scientific), followed by RNA clean up with the Agencourt RNAClean XP beads (Beckman Coulter). RNA samples were enriched for mRNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Libraries were constructed using ScriptSeq v2 RNA-Seq kit (Illumina), with library quality verified on the Agilent 2200 TapeStation System. Sequencing was performed using Illumina HiSeq SR50.