GSM3318633: DU145-CTL_2; Homo sapiens; RNA-Seq
Total RNA was extracted by using the mirVana RNA Isolation Kit (Ambion) according to the manufacturer's protocol. Three independently repeated experiments were performed for each cell line condition. The quantity and quality of the RNA samples were assessed by Nanodrop 2000 as well as Agilent 2100 Bioanalyzer before sent for AmpliSeq Transcriptome Sequencing. cDNA was synthesized from 100 ng of total RNA using the SuperScript® VILOTM cDNA Synthesis kit and amplified with Ion AmpliSeq™ technology. Barcoded cDNA libraries were diluted to 100pM, equally pooled, and amplified on Ion Torren OneTouch2 instrument using emulsion PCR. Then, templated libraries were subjected for sequencing of > 20,000 RefSeq transcripts using the Ion Torrent Proton™ sequencing system.