GSM1524261: PolII_Chia-PET_rep2; Mus musculus; ChIA-PET
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. DNA was end polished with T4 DNA polymerase (NEB). To assess the degree of intermolecular proximity ligation end polished DNA was divided into 2 aliquots and each ligated to linkers (A or B). The two samples were then joined together for proximity ligation under dilute conditions. Following ligation samples were treated with Mme1 to release paired end tag (PET) constructs. PET constructs were amplified and submitted to sequencing on Illumina Genome Analyzer II.