GSM4502432: WT_02; Homo sapiens; RNA-Seq
TRIzol was used to isolate total RNA. The RNA seq libraries were prepared with TruSeq RNA Library Prep kit v2 as per protocol instructions. Resulting libraries concentration, size distribution and quality were assessed on a Qubit fluorometer with a dsDNA high sensitivity kit and on an Agilent 2100 bioanalyzer using a DNA 7500 kit. Then libraries were normalized, pooled and quantified with a KAPA Library quantification kit for Illumina platforms on a ABI StepOnePlus qPCR machine, then loaded on a high output flow cell and paired-end sequenced (2x75 bp) on an Illumina NextSeq 550 next generation sequencer (performed at the NYUAD Sequencing Center).