GSM4502420: RNASeq_Ly49H_KO_D0_01; Mus musculus; RNA-Seq
Spleens were removed 2 days post MCMV infection, and Ly49H+ NK cells (TCRb- CD3e- NK1.1+ DX5+ Ly49H+) were sorted. Each sample represents an individual mouse. RNA was isolated using PicoPure RNA Isolation Kit (Cat# KIT0204 - Thermo Fisher) according to manufacturer's protocol. After RiboGreen quantification and quality control by Agilent BioAnalyzer, 2ng total RNA with RNA integrity numbers ranging from 9.4 to 10 underwent amplification using SMART-seq V4 Ultra Low Input RNA kit (Clonetech Cat# 63488), with 12 cycles of PCR. Subsequently, 10 ng of amplified cDNA was used to prepare Illumina HiSeq libraries using KAPA Hyper Prep DNA library preparation kit (KAPA Biosystems KK8504) using 8 cycles of PCR.