GSM2573313: E2M0c; Mus musculus; miRNA-Seq
Total RNA from unstimulated BMDMs was extracted using and miRNA isolation Kit (Qiagen) followed by DNase treatment to remove genomic contamination using RNA MinElute Cleanup (Qiagen). The purity and integrity of total RNA sample was verified using the Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA). rRNA was depleted from RNA samples using Ribo-Zero rRNA Removal Kit (Illumina). Small RNA libraries from WT BMDMs were performed TrueSeq Small RNA Library preparation (Illumina) and were sequenced on Illumina HiSeq 2000 platform (2 x 50bp read length).