GSM1524908: Col4a3 HET rep1; Mus musculus; RNA-Seq
Total RNA from tissue was extracted as per manufacturer’s instructions (miREASY kit, Qiagen). RNA quality was assessed using BioAnalyzer (Agilent). Illumina’s TruSeq RNA Sample Preparation v2 kit was used to construct libraries. Briefly, 150ng of total RNA input was used. The libraries were quantified using KAPA library quantification kit RNA libraries were prepared for sequencing using standard Illumina protocols