GSM2411135: ChIP-Seq analysis of HSF1 in P12 cells (rep1); Homo sapiens; ChIP-Seq
Twenty million cells were used for the ChIP and precipitated using 5 micrograms of antibody (cell signaling, 4356) against human HSF1. Twenty million cells were used for the ChIP and precipitated using 5 micrograms of antibody (santa cruz biotechnology, N-262) against human MYC Libraries were generated as described before (Ntziachristos et al., Nat Med, 2012), including end-repair, A-tailing, adapter (Illumina Truseq system) ligation and PCR amplification.