GSM4183315: U2OS/MTX300 control shRNA cells; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIZOL Reagent (Cat#15596-018，Life technologies, Carlsbad, CA, US) following the manufacturer's instructions and checked for a RIN number to inspect RNA integration by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US).Qualified total RNA was further purified by RNeasy mini kit (Cat#74106, QIAGEN, GmBH, Germany) and RNase-Free DNase Set  (Cat#79254, QIAGEN, GmBH, Germany).. RNA was quantified using a NanoDrop ND-100 UV-VIS spectrophotometer RNA libraries were prepared for sequencing using standard Illumina protocols