Mangrove soil bacterial communities from nutrient addition field experiments
A paired-end dual-index sequencing approach was used. The V4 region of the 16S rRNA gene was amplified using a one-step PCR protocol with 515F-806R primers (Caporaso et al. 2011) containing Illumina adapter sequences and 8 bp index sequences (Kozich et al. 2013; Griffiths et al. 2018).