GSM3553664: DRAL 26m Liver Rep3; Mus musculus; RNA-Seq
We isolated RNA from liver and epididymal white adipose tissue (WAT) of AL, DR, ALDR and DRAL mice at old age, as well as AL and ALDR mice at young age (three to five biological replicates each). RNA was isolated using Trizol Reagent (Thermo Fisher Scientific, Germany) according to the manufacturer's protocol before treating samples with DNAse using the TURBO DNA-free Kit (Thermo Fisher Scientific, Germany). RNA quality was measured using the Agilent TapeStation System (Agilent Technologies, Germany). RNA-seq library preparation and sequencing was performed by the Max Planck-Genome-Centre Cologne, Germany (http://mpgc.mpipz.mpg.de/home/). According to the facility's procedure, stranded TruSeq RNA-seq libraries were prepared as described in (Sultan et al., 2012), using 3 μg of rRNA-depleted RNA as input. Multiplexed libraries were sequenced with 2x40 mio, 100 bp paired-end reads on an Illumina HiSeq2500 (Illumina, San Diego, California, USA). Liver RNA-seq data for young ALDR, young AL, old DR and old AL fed mice were previously published (Hahn et al., 2017) and is publically available under the Gene expression omnibus ID GSE92486. Stranded, paired-end, total RNA-seq