GSM4185161: Aortic VSMCs, APOE1; Mus musculus; RNA-Seq
Aortas were lysated before subsequent selection with mouse CD31 and CD90.1 microbeads, with cell sorting taking place in an autoMACS Pro Separator (Miltenyi Biotec). Total RNA was extracted using RNeasy spin columns (Qiagen). Library was constructed by using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® according to manufacture's protocol.