Illumina HiSeq 2500 sequencing; Akkermanisa muciniphila response to oxygen exposure
Akkermanisa muciniphila response to oxygen exposure
In fermentor on mucus medium Total RNA was isolated by a method combining the Trizol® Reagent and the RNeasy Mini kit (QIAGEN GmbH, Hilden, Germany) Library construction for whole-transcriptome sequencing (RNA-Seq) was done by ScriptSeq™ v2 RNA-Seq Library Preparation Kit in combination with ScriptSeq™ Index PCR primers (Epicentre, Madison, WI, USA) according to the manufacturer’s instructions