WGS of Mus musculus: fallopian tube
Organoid DNA was extracted using the DNeasy Blood & Tissue Kit (Qiagen), according to the manufacturers instructions. Libraries were prepared using the Nextera DNA Flex Library Kit (Illumina). The manufacturers protocol was miniaturized by reducing reactions to one fourth of the recommended volumes. Following PCR amplification (5 cycles total), water (38 ul) was added to the amplified material (12.5 ul) to increase the volume to 50ul for the final 1x Ampure XP bead cleanup (Beckman Coulter).