GSM2412288: Wildtype C; Arabidopsis thaliana; miRNA-Seq
Total RNA of both wildtype and mutant leaf tissue was extracted using TriReagent (Ambion) following the manufacturer's protocol. Small RNA (sRNA) of each sample was further enriched using the mirVana miRNA isolation kitTM (Ambion) according to the manufacturer's instructions. The sRNA libraries were constructed using 1 μg of enriched sRNA fraction based on the previously published protocol by Xu et al., 2015.