GSM2671931: GSCs-U87MG MRS1220 hypoxia rep2 (condition D); Homo sapiens; RNA-Seq
Total RNA was isolated using a commercial kit, Nucleospin RNA ii mn (Macherey-Nagel) following Library preparation for RNA-seq was performed using the TruSeq RNA Sample Preparation Kit (Illumina, Cat. RS-122-2102) using at least 0.5 ug of total RNA and following the manufacturer’s instructions. Library quantitation was performed by qPCR using the Library Quant Kit Illumina GA (KAPA, Cat KK4824). The size range of the libraries was determined using the High Sensitivity NGS Kit on the Fragment Analyzer (Advanced Analytical Technologies, Cat DNF-474).