D. discoideum total tRNA, rep 1
Total RNA of D. discoideum cells were isolated using TRIzol. A 3'-adapter was fused to the small RNA preparation by truncated T4 RNA ligase 2. After cDNA synthesis, a second adapter was ligated to the cDNA 3'-ends, the reaction products were amplified by PCR. High-throughput analysis of the libraries was done as single end run (150 nt) on a Illumina MiSeq System with the custom primer (5'-CACTGTCGGTACCGAGCTTGCATGGAGTCCTA-3').