GSM3931858: H2O.RHYTHM.8.1; Mus musculus; RNA-Seq
RNA was isolated from 10-20mg of liver tissue using trizol reagent and purified on the Zymo Direct-Zol RNA miniprep kit. RNA quality was assessed by Bioanalyzer (Agilent). RNAs with a RNA Integrity Number (RIN) greater than 8 were used for library prep. Libraries were constructed from 250 ng of RNA using the NEBNext RNA Ultra Directional library prep kit from NEB. Average library size and concentration were determined by Bioanalyzer and qPCR (NEBNext Library Quant kit), respectively, prior to pooling.