16S rRNA amplicons MBBR Reactor 2 influent day 226
Biomass from a 1mL sample was collected by centrifugation andd removal of supernatant. DNA was extracted using the MP FastDNA spin kit (MP Biomedicals, USA) according to the manufacturer's protocol. Samples were amplified with 16S bacterial primers PRK341F and PRK806R targeting the V3 and V4 region. Pcrproducts were purified using AMPure beads (Beckman-Coulter) prior to index PCR and sequencing by Illumina MiSeq.