GSM3318870: IL4_5; Mus musculus; RNA-Seq
Qiagen RNeasy Plus Micro Kit was used to isolate total RNA according to manufacturer's instructions. Quality control of RNA samples was performed using RNA 6000 Nano Kit on an 2100 Bioanalyzer (Agilent). Sequencing libraries were prepared at the Core facility Genomics, Medical University of Vienna using the NEBNext Ultra™ Directional RNA Library Prep Kit for Illumina according to manufacturer's protocols (New England Biolabs). Libraries were QC-checked on a Bioanalyzer 2100 (Agilent) using a High Sensitivity DNA Kit for correct insert size and quantitated using Qubit dsDNA HS Assay (Invitrogen). Pooled libraries had an average length of 330-360bp and were sequenced on a NextSeq500 instrument (Illumina) in 1 x 75 bp sequencing mode.