GSM3996907: 223mut_27hpf_EC1; Danio rerio; RNA-Seq
Trunk tissue was dissected from 27 hpf zebrafish embryos under  0.1% tricaine anesthesia and then disassociated into a single cell suspension using 0.25 mg/ml liberase for 45 minutes. Tg(kdrl:hras-mCherry)s896 endothelial cells were isolated from the single cell suspension via FACS into 0.04% BSA in PBS and applied to the 10X genomics single cell RNA sequencing platform. 10 X genomics Chromium Next GEM Single Cell 3' Library Construction Kit v2.1