RNA-Seq of Homo sapiens: MCF-7 cell line
Extract protocol - Total MCF-7 RNA was ordered and received from Ambion/Life Technologies AM7846 as starting material. Library construction protocol - Illumina TruSeq RNA Library Prep Kit after ribosomal depletion using Epicentre RiboZero kit and ~600bp shearing. The 2x100bp data was collected on the HiSeq 2500 using standard v2 reagents.