GSM2576091: WT_3AT, replicate 1; Neurospora crassa; RNA-Seq
Ribosome-protected mRNA fragments were prepared for sequencing essentially as described in Ingolia et al (2012),Nature Protocols, except that 50 A260 units of lysate was used, nucleic acid pellets recovered from each step were washed with 80% ethanol, and the rRNA depletion step was omitted.