GSM4502379: WT CML_1; Mus musculus; RNA-Seq
LT CML stem cells isolated from Gdpd3+/+ tet-CML-affected mice and Gdpd3-/- tet-CML-affected mice were directly sorted by FACS Aria III cell sorter (BD Biosciences) into 500μl Isogene solution (Nippon Gene, Co. Ltd., Toyama, Japan). RNA extraction and sequencing were performed by DNAform (Yokohama, Japan). The RNA quality was confirmed using an Agilent 2100 Bioanalyzer (Agilent Technologies). All RNA samples had an RNA integrity number of >7.5 and exceeded the quality threshold for RNA sequencing. Libraries were constructed from total RNA using the SMART-Seq® v4 Ultra Low Input RNA Kit for Sequencing (Takara Clontech, Kusatsu, Shiga, Japan). Full-length double-stranded cDNA libraries were fragmented by transposase using the Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA, USA). Paired-end reads of 150 bases were generated using HiSeq (Illumina).