rDNA of Symbiodinaceae of Diploria labyrinthiformis: symbiotic algae
High-throughput sequencing of ITS2 rDNA. ITS2 region amplified using ITS-D and ITS2rev2 with Illumina overhang adapters. Amplified DNA was cleaned using SPRI beads and used as template for an index PCR in which unique combinations of Nextera XT index primers were used for each sample. Eight cycles of PCR were performed, libraries were cleaned, normalized, and pooled for sequencing.