Illumina HiSeq 2000 sequencing; BPA impacts on HEK 293 cells
BPA impacts on HEK 293 cells
The human embryonic kidney 293 cells (HEK 293) were cultured at 37C in 5% CO2 as adherent monolayer in Dulbecco modified Eagle medim(DMEM) (Hiclone) supplemented with L-glutamine and 10% fetal bovine serum (FBS) (Hiclone). BPA powder was dissolved in the dimethyl sulfoxide (DMSO) and added to culture medium. The final concentration of BPA and DMSO is 10-6 M and 10-3 M, respectively. For BPA treatment, cells were treated with 10-6 M BPA for 48h. Meanwhile, cells cultured in BPA-free medium were used as the control. Total RNA was extracted from each sample using Trizol reagent (Invitrogen) according to the manufacturers instructions. mRNA enrichment and library preparation were performed at BGI-Shenzhen (sequencing service provider).