Global Transcriptome analysis of the Klebsiella pneumoniae strain cifa_HP1 heat shocked at 50 degree celsius
Bacterial culture of Klebsiella pnemoniae was grown on TSB media and RNA was isolated from cells heat shocked at 50 degree celsius for 15 minutes. Ribodepleted total RNA(mRNA) was isolated and then fragmented using RNase III enzyme. Fragmented mRNA was hybridized with adapters followed by ligation. Reverse transcription was carried out to obtain cDNA and then size selected libraries were amplified. Finally transcriptome library was quantified and qualified using bioanalyzer