RNA-Seq of octopus vulgaris:paralarva 10 dph
Double-stranded cDNA libraries were prepared using the Kapa Stranded mRNA-seq kit (KK8420) following the manufacturer instructions with a mean library insert size of 201300 bp. Briefly, RNA samples were first purified with two oligo-dT selection (poly(A) enrichment using oligodT beds), and then fragmented and reverse transcribed into double-stranded complementary cDNA. A total of 12 libraries were paired-ended sequenced (2  150 bp) in an Illumina HiSeq 4000 platform from individual samples.