GSM3243792: Tbk1+/-;Ripk1D138N/D138N rep3; Mus musculus; RNA-Seq
Cells were lysated, total RNA was harvested using Quick-RNA MiniPrep Plus kit (ZYMO) Each library was prepared using the Smart-seq2 protocol with a tagged oligo (dT) primer to make first strand cDNA from 1 ng total RNA  input. Double-stranded cDNA was generated for library construction with 12 cycles of PCR and 0.3 ng of cDNA was used as input to the NexteraXT kit (Illumina). Libraries were sequenced with a NextSeq (Illumina) to generate 50 base paired end reads. Reads were aligned to the mm10 UCSC mouse transcriptome and expression values were determined using RSEM.