GSM3318743: MIM2109_2; Medicago truncatula; ncRNA-Seq
Materials were collected and flash frozen in liquid nitrogen. Total RNA was extracted by Plant RNA reagent (Ambion, Life Technologies, USA) according to manufacturer's protocol. Small RNA libraries were constructed from 1 microgram of  total RNA using Illumina Truseq small RNA sample preparation kit (RS-200-0012). RNA-seq libraries were constructed using the Illumina TruSeq RNA sample preparation kit (RS-122-2001). PARE libraries were constructed according to the protocols by German et al., 2009 and Zhai et al., 2014.