GSM4183296: ATAC Zld mut rep2; Drosophila melanogaster; ATAC-seq
Embryos produced from either wild-type (D. melanogaster.ZH-86Fb) or mutant sh opa and sh zld were collected on yeasted apple juice agar plates. Individual embryos were selected from plates , and nuclear morphology was observed under a compount microscope at 20x magnification. Temperatures for sample collection were maintained between 22°C and 24°C to minimize variation in cell cycle timing. Under these conditions, cell cycle times were indistinguishable. Cell cycles were timed from the onset of anaphase of the previous cell cycle. The staging of samples at 3-min intervals relies on the synchrony of nuclear divisions  in the early Drosophila embryos. Each embryo was hand-selected and hand-dechorionated for the analysis. For ChIP-seq, anti-Opa antibodies were raised in two rabbits (E990 and E991) against a truncated recombinant protein spanning from amino acids 125-507 of Opa. Fragmentation and amplification of ATAC-seq libraries were performed essentially as described previously (Buenrostro et al., 2015). Prepared libraries were subject to single-end sequencing using an Illumina HiSeq2500.