16S of V6: soil
Targets the V6 region of the bacterial 16S ribosome. Each sample in a group of 66 uniquely indexed for individual identification. After amplification, PCR amplicons from each of the 66 samples were purified and quantitated and pooled at equimolar proportions into a single pool for sequencing. Each pool was loaded onto a single lane of a flow cell for cluster generation. The samples were run on a 125bp paired end sequencing run on the Illumina HiSeq2500.