Lake Baikal neuston, August 2013
Subjects of investigation were bacterioneuston communities of Lake Baikal. Samples from the surface microlayer were collected in August 2013. Sampling was performed using the method of metal mesh screen. Samples of water (50 mL) were filtered through polycarbonate membrane filters (pore size 0.22 ?m) (Millipore, United States). Total DNA was extracted using DNA-sorb B kit (Central Research Institute of Epidemiology, Russia). Fragments of the 16S rRNA gene containing three variable regions V1V3 were amplified using eubacterial primers V1-9F (5'-X-AC-GAGTTTGATCMTGGCTCAG-3') and V3-541R (5'-X-AC-WTTACCGCGGCTGCTGG-3'), where X is an 8 nucleotide long barcode uniquely designed for each sample followed by a common linker AC (Chun et al., 2010). Metagenomic sequencing of the 16S rRNA gene amplicons was performed using the 454 Genome Sequencer FLX Titanium (Roche) in Limnological Institute SB RAS, Russia. MID1 - B_P13; MID2 - B_17AM; MID3 - B_17BM; MID4 - B_15AM; MID5 - B_8AM; MID6 - B_4AM0; MID7 - B_7AM; MID8 - B_7BM; MID9 - B_1AM; MID10 - B_11AM; MID11 - B_4AM1