NextSeq 550 paired end sequencing
Analysed via Jovian version 0.9.7.1 (https://github.com/DennisSchmitz/Jovian). Reads aligning against the human genome (GRCh38) were removed. Quality filtering -m relaxed.
Centrifugation. Filtration. Saponine treatment. Endonuclease treatment. Lysis. MagNa Pure 96 extraction. Random primed cDNA synthesis. Purification. Nextera XT lib prep.