WGS of Escherichia coli: pathogenic isolate: Sample WU43
"We sheared 500ng of total DNA to  approxiamately 300 bp fragments fragments in nine rounds of shearing of ten minutes each on the BioRupter XL. We the DNA concentrated using the Qiagen MinElute PCR purification kit per the manufacturer's protocol, followed by End Repair of the sheared DNA fragments using NEB T4 polymerase. Barcoded adapters were then added to the solution before purification using the Qiagen MinElute PCR purification kit. The DNA fragments were then size selected to 250-300 bps on a 2 percent agarose gel. The purified DNA was enriched by PCR before pooling and sequencing."