Transcriptional changes of Rice in RBSDV-infected (diseased) sample (D2-1)
To monitor the response of the rice transcriptome to RBSDV infection, a RBSDV-susceptible rice cultivar, Kangtiaowuyujing 3 (KTWYJ3) (Oryza sativa L. ssp. Japonica) was used. Rice seeds were planted in a plot and left under field infection pressure (from indigenous viruliferous SBPHs) without protective net in order to obtain RBSDV-infected plants. Thirty days later, the seedlings were transplanted at the same field and covered with insect-proof protective net. RNA was extracted from diseased rice plant (D2-1) five weeks after transplanting.