GSM4502467: Prm2-WT2; Mus musculus; RNA-Seq
Total RNA was extracted from testicular tissue after removal of the tunica albuginea using TRIzol™ reagent according to the manufacturer's protocol (Thermo Fisher Scientific). RNA sample quality control and library preparation were performed by the University of Bonn Core facility for Next Generation Sequencing (NGS), using the QuantSeq 3´-mRNA Library Prep (Lexogen, Greenland, NH, USA). RNAseq was performed on the Illumina HiSeq 2500 V4 platform, producing >10 million, 50bp 3'-end reads per sample.