GSM1406367: pre-osteoblasts + IL-17A replicate 1; Mus musculus; RNA-Seq
RNA was extracted from the wells after 7 days with Trizol. 1 µg of total RNA samples was used as provided by the user. RNA Integrity Numbers were in the range 9.7 to 10 when assayed on an Agilent 2100 Bioanalyzer. PolyA+ fractions were randomly fragmented, converted to double stranded cDNA and processed through subsequent enzymatic treatments of end-repair, dA-tailing, and ligation to adapters as in Illumina's "TruSeq RNA Sample Preparation V2  Part # 15026495 Rev. B February 2012). Adapter-ligated library was completed by 8 cycles of PCR with Illumina PE primers. The resulting purified cDNA library was applied to an Illumina flow cell for cluster generation (TruSeq cluster generation kit v5) and sequenced on the Genome Analyzer IIx with SBS TruSeq v5 reagents by following manufacturer's protocols.