GSM4073418: human_302OE2; Gallus gallus; miRNA-Seq
Total RNA was extracted from sorted cells using the Qiagen miRNeasy Mini Kit protocol as stated except that Trizol-LS was used instead of Qiazol and RNA was eluted off the column in 11ul. half of the toal RNA obtained was used as input for the NEXTflex® Small RNA-Seq Kit v3 from Bioo Scientific. The protocol was followed as stated and gel extraction was used for size selection