GSM1895471: EU34_NI2 (miRNA); Homo sapiens; miRNA-Seq
Total RNA was extracted from the non-infected and infected macrophages using the miRNeasykit (Qiagen). RNA quantity was evaluated spectrophotometrically, and the quality was assessedwith the Agilent 2100 Bioanalyzer (Agilent Technologies).   Only samples with no evidence for RNA degradation (RNA integrity number>8) were kept for further experiments. miRNA libraries were prepared using the Illumina TruSeq Small RNA library protocol. We multiplexed 36 libraries per lane and sequenced the pool in two lanes with single-end 100bp reads