RNA-seq of mus musculus: adult female intestine DCs
After the total RNA quality detection and DNase I treatment, we used magnetic beads (Invitrogen, CA, USA) to isolate mRNA. After mixing with the buffer, the mRNA is divided into short fragments. Then we synthesized the cDNA using the mRNA fragment as a template. The short fragment was then purified and resolved with EB buffer and single nucleotide A (adenine) was added for end repair. In addition, we use specific adapters to connect short segments together. After agarose gel electrophoresis, the appropriate fragment was selected as a template for PCR amplification.