GSM3615396: AHCY-KO-MEF-CT28-2; Mus musculus; RNA-Seq
Total RNA was isolatedfrom MEF cells at the indicated time points using TRIzol reagent. RNA was extracted from SCN punches using the RNeasy Micro Kit. From MEF cells libraries were prepared from three biological replicates according to the TruSeq RNA Library Prep Kit v2. From SCN samples, libraries were generated from four biological replicates using the SMARTer Stranded total RNA-seq kit v2, pico input mammalian according to manufacturer's instructions.