mouse newborn cardiac myocyte cells rep1: RNAseq SE
Single cell suspensions were derived from cardiac tissues by enzymatic digestion. Cardiomyocytes were sorted by FACS according to cell size. RNA was extracted using RNeasy Kit (Qiagen). Polyadenylated RNA was isolated from 500ng of total RNA with magnetic beads (NEBNext Poly(A) mRNA Magnetic Isolation module). Libraries were constructed (NEBNext* Ultra RNA Library Prep Kit for Illumina) according to manufacturerM-bM-^@M-^Ys instructions and size selection was performed with AMPure* (Beckman Coulter, Inc.) XP Beads. 13 PCR cycles were used for library amplification.