GSM2572936: R2232.DG.OG; Rattus norvegicus; RNA-Seq
Rats were anesthetized with isoflurane, decapitated and the brain was rapidly removed. The hippocampus was isolated, and the CA1, CA3 and dentate gyrus (DG) regions were dissected. The collected tissue was immediately frozen in liquid nitrogen and stored in -80ºC. Total RNA was isolated using the miRNeasy Mini kit (Qiagen, cat #: 217004). For the Ion Proton library construction, 250ng of total RNA was used for polyA selection with the Dynabeads mRNA DIRECT Micro kit (Thermo Fisher, cat #: 61021). Transcriptome libraries were made with the Ion Total RNA-seq Kit v2 (Thermo Fisher, cat#: 4475936) with the addition of the Ion Xpress barcodes for multiplex sequencing (Thermo Fisher, cat#: 4475485). For Illumina HiSeq2500 library construction, 250ng of total RNA was used as the input material for the Illumina's TruSeq RNA Sample Preparation Kit v2 (Illumina Inc.) following the manufacturer's protocol where mRNA was selected using poly-T oligo magnetic beads. All libraries were quantified in an Agilent 220 Tapestation system.