GSM1625680: RRMS, 2961-TA-40; Homo sapiens; RNA-Seq
Peripheral whole blood was collected into PaxGene tubes. RNA was isolated per the manufacturer's supplied protocol. Total RNA isolated from PaxGene tubes was purified with the RNeasy MinElute Cleanup kit (Qiagen) using an on-column DNase treatment to ensure absence of genomic DNA contamination according to the manufacturer’s supplied protocol. 2343 and 2961 libraries were constructed using the Illumina TruSeq Stranded mRNA kit (Cat. No. RS-122-2101) following the manufacturer's supplied protocol.