Illumina HiSeq 2000 sequencing; ChIP-sequencing of murine T-cell acute lymphoblastic leukemia (T-ALL) cell lines to investigate histone methylation profiling of lymphocyte deficient for Tet2 and mutated for DNMT3A
ChIP-sequencing of murine T-cell acute lymphoblastic leukemia (T-ALL) cell lines to investigate histone methylation profiling of lymphocyte deficient for Tet2 and mutated for DNMT3A
Cell lines were established after culture on MS5 feeder in MEMa medium supplemented with 10% FBS and 10ng/mL of murine IL7, FLT3L and SCF. MO467 cells were then grown in RPMI 1640 supplemented with 10% FBS and R152 with RPMI 1640 supplemented with 10% FBS and 10 ng/ml of murine IL7 SPRIworks (Beckman Coulter)