GSM2411617: wt2_wk24; Mus musculus; RNA-Seq
Total RNA was isolated from cells using miRNAeasy Kit (Qiagen) according to the manufacturer’s protocol. An additional DNaseI digestion step was performed to ensure that samples were not contaminated with genomic DNA. Reverse transcribed PCR (RT-PCR) was performed using a OneStep RT-PCR Kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions. Libraries were prepared using an Illumina TrueSeq RNA prep kit.