GSM3389539: input RNA, parental cells; Homo sapiens; OTHER
RNA was extracted with Trizol (Invitrogen) and subsequently purified using RNeasy Midi Kit (QIAGEN). Purified total RNA was fragmented in freshly prepared RNA fragmentation buffer (10 mM Tris-HCl, pH 7.0, 10 mM ZnCl2) Library preparation and next generation sequencing were performed by the Genomics Facility at the Wistar Institute using ScriptSeq complete Gold kit (Epicentre) following the manufacturer's instructions.