RNA-seq of PA14 small RNA
For small RNA-seq, around 300 nanograms of small RNA from each sample was  first treated with RNA 5' Pyrophosphohydrolase (New England Biolabs, MA) at  37oC for 30 minutes, then converted to Illumina sequencing libraries using  the PrepX RNA-seq library preparation protocol on the automated Apollo 324TM  NGS Library Prep System (Takara Bio, CA).