GSM2574088: 54402_Nras_1; Mus musculus; RNA-Seq
SLAM-HSCs were FACS-sorted into TRIzol (Ambion). Total RNA was isolated using the AllPrep DNA/RNA kit from Qiagen (Valencia, CA) according to manufacturer’s instructions. Ribosomal RNA (rRNA) and mitochondrial RNA (mtRNA) was removed using the RiboGone-Mammalian kit (Takara 634847) according to manufacturer's instructions. RNA-seq libraries were prepared using the SMARTer Stranded RNA-seq Kits (Takara 634837) according to the manufacturer's instructions.