GSM3555680: B. natans dark 3; Bigelowiella natans; ncRNA-Seq
Cultures were harvested by centrifugation and flash-frozen in liquid nitrogen. Low molecular weight RNA was extracted using the mirVana miRNA isolation kit, using the protocol for enrichment of <200 nt RNAs provided by the manufacturer (Thermo Fisher Scientific). For G. theta sRNA, an aliquot of one of the replicates was treated with Terminator Exonuclease, using the standard protocol (Epicentre). The SMARTer smRNA-seq kit (TaKaRa) was used to prepare barcoded sRNA-seq libraries (TruSeq HT indexes) in preparation for Illumina sequencing. Inserts were enriched for amplicons <150 bp using Agencourt AMPure XP beads (Beckman Coulter). The G. theta and B. natans libraries were sequenced separately on a MiSeq instrument.
GEO Accession
GSM3555680