single-cell CITE-seq HTO of homo sapiens: 7 adult peripheral lymphocyte populations: Sample single_cell_experiment
Cell types from each donor were each stained with human Hashtag antibody 1 (https://www.biolegend.com/en-us/products/totalseq-a0251-anti-human-hashtag-1-16080) and ran as described for mRNA through 10X machine. HTO (CITE-seq protocol Stoeckius et al) libraries were prepared. We used the following barcodes for each cell type/donor. CD4:  A0251 GTCAACTCTTTAGCG; CD8: A0252 TGATGGCCTATTGGG; MAIT: A0253 TTCCGCCTCTCTTTG; NKT: A0254 AGTAAGTTCAGCGTA; Vd1: A0255 AAGTATCGTTTCGCA; Vd2: A0256 GGTTGCCAGATGTCA; NK: A0257 TGTCTTTCCTGCCAG; Donor 1:  A0258 CTCCTCTGCAATTAC; Donor2: A0259 CAGTAGTCACGGTCA. Libraries were sequenced together with mRNA libraries and demultiplexed.