GSM4279851: 1014; Mus musculus; RNA-Seq
The left ventricle was immediately isolated after extraction, flash frozen in liquid nitrogen, and pulverized cold. Total RNA was isolaged from pulverized tissue using miRNeasy minikit (Qiagen), according to manufacturer's instructions. cDNA libraries were generated from the isolated mRNA using an NEBNext Ultra RNA library prep kit for Illumina (New England Biolabs) and a unique 6 nucleotide index primer (NEBNext multiplex oligos for Illumina) was incorporated into each sample.  The library construction was done according to NEB instructions, modified for use with a Beckman Biomek 4000 at the Florida State University Biological Sciences core lab.  The unique index (barcode) was added to each library to multiplex the sixteen libraries into two lanes of the sequencing run. The multiplexed sample was quantified with qPCR (Kapa Biosystems) specific for Illumina sequencing primers and the average fragment size was determined with a Bioanalyzer high sensitivity DNA chip (Agilent Technologies).  12 pM of the pooled sample was sequenced, with single end, 100 base reads on an Illumina HiSeq 2500 located in the Translational Science Laboratory at the College of Medicine, Florida State University.  The pooled data were demultiplexed into individual sample data.
GEO Accession
GSM4279851