GSM2579082: RNA pol II ChIP-seq yRpb1  rpb1-N488D; Saccharomyces cerevisiae; ChIP-Seq
cross-linked Chromatin was sonicated with a Bioruptor 1-2 mg of cross-linked extract was immunoprecipitated, and prepared for illumina library construction by repair with Klenow, T4 DNA polymerase and T4 polynucleotide kinase, A-tailing with Klenow exo-, ligation to branched adaptors, gel purification of ~200bp fragments, and amplification (18cycles) with Illumina Tru-seq primers using Phusion DNA polymerase.
GEO Accession
GSM2579082