cellulose and lignin decomposition in red soil
Soil DNA was extracted with FastDNA Spin kit for Soil DNA extraction kit. The V3–V4 region of the 16S rRNA gene was amplified in triplicate using the barcoded 515fF and 806R primer set and ITS2 with ITS3 and ITS4. PCR products were quantified, purified and sequenced by Ion Torrent platform for assessment of bacterial diversity and community structure. We designed the experiment to explore decomposition of cellulose and lignin in a red soil receiving long-term organic and chemical fertilizer.