D2.2-2.21
Following the manufacturers recommendations and index codes, sequencing libraries were generated using TruSeq DNA PCR-Free Sample Preparation Kit (Illumina, USA). The library quality was assessed by Qubit@2.0 Fluorometer (Thermo Scientific) and Agilent Bioanalyzer 2100 system. At last, the library was sequenced on IlluminaHiseq 2500 platform, and 250 bp paired-end reads were generated.fined