GSM3616072: DEK-Treated Lineage Depleted Bone Marrow, Replicate 3; Mus musculus; RNA-Seq
Mouse BM was isolated from the femur of the indicated mouse strain immediately prior to use by flushing either in PBS (for transplantation studies; Lonza; Walkersville, MD, USA) or in Iscove's Modified Dulbecco's Medium (IMDM; Lonza) with hepes, L-Glutamine, and 10% fetal bovine serum (FBS; Fisher Scientific; Waltham, MA, USA) for all culture experiments. Lineage depletion of mouse BM was performed using a mouse lineage cell depletion kit following the manufacturer's protocol (Miltenyi Biotec; Aurburn, CA, USA) using two sequential columns. The All Prep DNA/RNA Mini Kit (Qiagen) was utilized to isolate RNA from cell lysates. RNase-Free DNase (Qiagen) was used to eliminate contaminating genomic DNA. Strand-specific libraries were prepared using the TruSeq Stranded mRNA kit (Illumina) according to the manufacturer's protocol.
GEO Accession
GSM3616072