Illumina seq of Pinus patula
The internal transcribed spacer 1 (ITS1) nuclear encoded ribosomal RNA of the endophytic community of the needles was amplified using ITS 1F and ITS 2. The DNA library was submitted to Inqaba Biotech (Pretoria, Gauteng, South Africa, www.inqababiotec.co.za) where Illumina MiSeq sequencing of the amplicons was performed.