RNA-seq of Lake Winnipeg adult Sander vitreus: Gill tissue
Gill tissue from each fish was homogenized using a TissueLyser (Qiagen) in Buffer RLT Plus (RNeasy Plus Mini Kit; Qiagen) and 350 ?l of the homogenate was used for the RNA extraction following manufacturers protocols. RNA quality was assessed using a Bioanalyzer (Agilent). Total RNA was used to prepare 48 separate cDNA libraries using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) barcoded with a NEBNext dual adaptors (New England Biolabs) prior to sequencing. All libraries were pooled and sequenced on an Illumina NovaSeq 6000 over one lane. Sequencing was conducted to produce 100 base pair paired-end reads for each individual.