AL-1 cells, Mitotic
For arresting AL1 cells in mitosic phase, AL1 cells were treated with Thymidine-nocodazole culture conditions . Culture cells were treated with 1% Trypsin at room temperature for 3 minutes, then Trypsin was quenched by addition of 10%FCS/AMEM. Cells were spun down and counted. 1x 106 cells were resuspended into 36ml of 10%FCS/AMEM. Restriction enzyme was DpnII