RNAseq of VLP Vpx treated - HIV exposed, GFPpos - resting CD4 T cells - healthy donor3
Total RNA extraction using GE Healthcare Illustra RNA mini kit was performed. RNA quality was analyzed on a Agilent Bioanalyzer 2100 with a RNA NanoChip. Standard TruSeq RNA Sample Prep, barcoded, Illumina was used to prepare the library. Sample were multiplexed (by 2) on Illumina HiSeq2000 slides which provides 75-100 million single-end reads of 100 nucleotides per sample.