GSM4230240: DMSO 6h_PER403R1; Homo sapiens; RNA-Seq
RNA was extracted using Qiagen RNeasy Mini plus kit. Genomic DNA was removed through rigorous DNase treatment with the Invitrogen TURBO DNA-free kit (Fisher Scientific; AM1907) ERCC ExFold RNA spike-in controls (Invitrogen; 4456739) were added according to manufacturer's instructions to 1 µg of purified RNA from each sample to enable fold change quantification in treated samples relative to vehicle condition.  Ribosomal rRNA (rRNA) was depleted using the NEBNext rRNA Depletion Kit (NEB; E6350L) according to manufacturer's instructions. Sequencing libraries were generated using the NEBNext Ultra II Directional RNA library kit (NEB; E7760S). Libraries were single-end 75 bp sequenced on an Illumina Nextseq 500
GEO Accession
GSM4230240