GSM1301734: OP50_N2_2; Caenorhabditis elegans; RNA-Seq
Purified total RNA were processed with MirVana kit (Invitrogen) for small RNA enrichment. Equal amount of small RNAs (30~40ug) were resolved on 15% TBE-Urea Acrylamide gels. Gels containing small RNAs of 20-26nt were cut out and purified with a small-RNA PAGE Recovery Kit (Zymo Research, R1070). Each small RNA sample was treated with TAP and processed with the ScriptMiner Small RNA-Seq Library Preparation Kit (Epicentre, SMMP1012). Libraries from different samples were indexed with RNA-Seq Barcode Primers (Epicentre, RSBC10948). Two biological samples of each condition (wild type +sel-1 RNAi, rde-12+sel-1 RNAi, wild type+OP50 and rde-12+OP50) were used for library preparation
GEO Accession
GSM1301734