GSM2150578: RNAseq_shoot_3; Arabidopsis thaliana; RNA-Seq
About 0.1 g of pulvernized root or shoot tissue was resuspended in 300 or 400 µl of polysome extraction buffer. The lysate was either treated with RNase to obtain ribosome footprints or used to isolate total RNA using Zymo RNA Clean and Concentrator kit. Ribosomes were isolated with size exclusion columns (illustra MicroSpin S-400 HR Columns, GE Healthcare, Cat. # 27-5140-01). Purified ribosome footprints and total RNA were then subjected to rRNA depletion. Libraries were constructed according to ARTseq/TruSeq Ribo Profile Library Preparation Kit manual
GEO Accession
GSM2150578