ZMB80739 Hyperolius chlorosteus voucher ZMB80739
Genomic DNA was extracted using a high-salt extraction method and 1700 ng total DNA was diluted in 110 lL of ultrapure H2O. A Bioruptor UCD-200 (Diagenode) was used to sonicate the samples on a low setting for 15 min, using 30 s on/30 s off cycling (100500 bp, average 200300 bp). Individual genomic libraries were prepared following Meyer & Kircher (2010). We used seven cycles of postadapter ligation PCR to enrich the libraries and incorporate a 7 bp P7 index, producing an average yield of 1750 ng total library DNA. Samples were pooled for capture reactions (56 genomic libraries) and contained 1500 ng of total starting DNA. MYbaits capture reactions were performed following the v2.3.1 manual with some modifications. The combined postcapture libraries were grouped into three sets (totalling 74, 91 and 92 libraries), pooled in equimolar amounts, and sequenced on three lanes of an Illumina HiSeq2500 with 100 bp paired-end reads.