GSM4279706: KIT-purified dSPG Rep4; Homo sapiens; RNA-Seq
For bulk RNA-Seq, total RNAs were extracted using the RNeasy Plus Micro Kit (Qiagen) according to the manufacturer's protocol.   For single cell RNA-Seq, cells were incubated in StemPro accutase solution for 5 min. Single cell suspension were diluted for loading on Chromium chip for 10X Genomics library preparation and subsequent sequencing. For bulk RNA-Seq, 10 ng of total RNA was subjected to prep the library using SMARTer® stranded total RNA-Seq kit v2 - pico input system (Takara Bio USA) per manufacturer's instructions.   For single cell RNA-Seq, Viable cells were washed once in PBS and resuspended in 0.04% BSA in PBS for loading on the 10x Chromium chip. Cell capturing, and library preparation was carried as per kit instructions (Chromium Single Cell 3′ Library Construction Kit v3).
GEO Accession
GSM4279706