GSM4230077: Un-Wounded_2_scRNA-Seq; Mus musculus; RNA-Seq
Normal un-wounded mouse skin and mouse wound were isolated, made into a single cell suspension, sorted for live cells, and then processed as per 10X Genomics 3' scRNA-Seq v1 protocol. Sorted cells were washed in PBS with 0.04% BSA and reseupended at a concentration of approximately 1000 cells/µl. Library generation for 10x Genomics v1 chemistry was performed following the Chromium Single Cell 3' Reagents Kits User Guide: CG00026 Rev B. Quantification of cDNA libraries was performed using Qubit dsDNA HS Assay Kit (Life Technologies Q32851) and high-sensitivity DNA chips (Agilent. 5067-4626). Quantification of library construction was performed using KAPA qPCR (Kapa Biosystems KK4824)
GEO Accession
GSM4230077