Illumina sequencing of Treponema pallidum tprK amplicon
The region of interest was amplified using a HiFi polymerase (Takara). The resulting PCR product was purified with 0.6x volumes of AMPure XP Beads (Beckman Coulter) and diluted to 1ng/ul. The library was prepared using the Nextera kit, cleaned with 0.6x volumes Ampure XP beads and sequenced on a 1x192bp MiSeq run.