Nextera: Pdia3-Vector-2
PCR amplicons were used for Nextera library preparation following the manufacturers protocols (Illumina). Briefly, purified PCR product was fragmented and tagged using the Nextera Amplicon Tagment Mix, followed by limited-cycle PCR with indexing primers and Illumina adaptors. After this amplification, DNA bands were purified with a gel extraction kit (Qiagen).