Illumina HiSeq 2500 paired end sequencing; Multi-tissue transcriptome associated with feed efficiency in Nellore cattle
100 bp paired-end RNAseq of poliA selected RNA on an Illumina HiSeq2500
TruSeq Stranded mRNA LT Sample Prep
assay type
RNA-seq of coding RNA
sample storage
frozen, -70 freezer
sample storage processing
cryopreservation in liquid nitrogen (dead tissue)
sampling to preparation interval
39
experimental protocol
https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf
extraction protocol
https://www.qiagen.com/se/resources/resourcedetail?id=14e7cf6e-521a-4cf7-8cbc-bf9f6fa33e24
library preparation location
Centro de Genomica Funcional ESALQ-USP Piracicaba Brazil
library preparation location longitude
-22.71
library preparation location latitude
-47.63
library preparation date
2016-12-14
sequencing location
Functional Genomic applied to Agriculture ESALQ-USP Piracicaba Brazil
sequencing location longitude
-22.71
sequencing location latitude
-47.63
sequencing date
2016-12-21
experiment target
polyA RNA
rna preparation 3' adapter ligation protocol
https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf
rna preparation 5' adapter ligation protocol
https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf
library generation pcr product isolation protocol
https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf
preparation reverse transcription protocol
https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf
library generation protocol
https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf
read strand
mate 1 sense
rna purity - 260:280 ratio
2.1
rna purity - 260:230 ratio
not collected
rna integrity number
8.9