Illumina HiSeq 1500 sequencing; RNA-seq of effect of Lysophosphatidic acid in ovarian cancer cell line OVCAR8 and macrophage-like cell line THP-1
RNA-seq of effect of Lysophosphatidic acid in ovarian cancer cell line OVCAR8 and macrophage-like cell line THP-1
OVCAR8 were obtained from NIH. THP-1 were obtained from ATCC. OVCAR-8 (2 x 10^5/12Well); cultured in RPMI1640 10 % FCS, 1% P/S over night; starvation in RPMI 1% BSA for 48 h; treatment Pretreatment: LPA-Mix  LPA 16:0 (1 µM) LPA 18:0 (0,25 µM) LPA 18:1 (0,5 µM) LPA 18:2 (1,625 µM) LPA 20:4 (1,625 µM) (5 µM) for the denoted period of time    NucleoSpin RNA Kit (Macherey-Nagel, 740955.250); DNA-free™ DNA Removal Kit (ThermoFisher/Ambion, AM1906); according to manufacturer's protocol Libraries were created using the Illumina Truseq mRNA kit v2 used according to the manufacturer's instructions.
Experimental Factor: compound
none
Experimental Factor: time
5
Experimental Factor: cell line
THP-1