GSM1963080: RNA_WTMEF_TPA_replicate1; Mus musculus; RNA-Seq
RNA-seq libraries were prepared using directional mRNA-Seq Library Prep v1.0 Protocol from Illumina with minor adjustments. The samples were collected using GenElute Mammalian Total RNA Miniprep Kit and DNA contaminants were removed by treating the samples with DNaseI (see section above). To reduce the ribosomal rRNA in the libraries, samples were processed using the Ribo-zero rRNA removal kit (Epicentre). The Kapa HiFi HotStart ready mix, which reduced the overall volume of the PCR, was substituted to the Illumina kit Phusion enzyme and the ratio for the Agencourt AMPure XP beads was adjusted accordingly. The standard PCR cycle suggested by the Illumina protocol was changed to match the quantity of the total RNA measured initially with the Bioanalyser.
GEO Accession
GSM1963080