GSM3692902: Sample_97L_P_AC_2; Homo sapiens; RNA-Seq
Take an appropriate amount of cell sample, and RNA was harvested using mirVana™ miRNA ISOlation Kit and Ambion-1561. Illumina TruSeq Stranded mRNA LTSample Prep Kit was used with  total RNA for the construction of sequencing libraries. After extracting total RNA from the sample and digesting the DNA with DNase, enrich the eukaryotic mRNA with magnetic beads with Oligo (dT) (if the prokaryote, remove the rRNA by the kit to enrich the mRNA); add the interrupting reagent The mRNA was broken into short fragments, and the broken mRNA was used as a template. A six-base random primer was used to synthesize a strand cDNA, and then a two-stranded reaction system was synthesized to synthesize a double-stranded cDNA, and the double-stranded cDNA was purified using a kit; The double-stranded cDNA was further ligated to the end, the A tail was added and the sequencing linker was ligated, and then the fragment size was selected, and finally PCR amplification was performed. The constructed library was tested with the Agilent 2100 Bioanalyzer and the Illumina HiSeqTM 2500 or Illumina HiSeq X Ten was used. The sequencer is sequenced to generate double-ended data of 125 bp or 150 bp. After passing the quality test, sequencing was performed using an Illumina sequencer.
GEO Accession
GSM3692902