GSM2756149: SQCC; Homo sapiens; ncRNA-Seq
Blood was collected from non-small cell lung cancer patients or healthy controls, and dispensed into an EDTA anticoagulation tube. Plasma was separated by centrifugation at 5 000 rpm for 5 minutes at 4℃. The clear supernatant was transferred to a labeled tube, and stored at -80℃. Exosomes from plasma were isolated using Ribo Exosome Isolation Reagent (for serum or plasma) (Ribobio) according to the manufacturer’s protocol. Then the total RNA in plasma exosomes was extracted and small RNA fractions were separated by electrophoresis. The small RNA fractions (<60nt) from plasma exosomes were ligated to 5’-adapters and 3’-adapters, and then reverse-transcribed and PCR amplified, with indexes included in the PCR reaction. PCR products were run on a 6% polyacrylamide gel and size-selected to include RNAs with an insert size < 51 nt. The DNA-containing index was eluted from the gel, precipitated, and dissolved in nuclease-free water. Small RNA inserts were sequenced using a HiSeq 2500 high-throughput sequencing system (Illumina).
GEO Accession
GSM2756149