ex1ex2_wt_24h singlet oxygen replicate3
Total RNA of the leaves inoculated by PDA plugs of A. alternata was extracted using the RNeasy Plant Kit (Qiagen), following the manufacturers instruction. The purity of RNA was veried by a NanoDrop 2000 spectrophotometer. The integrity of RNA was evaluated by RNA 6000 Nano Kit of Agilent 2100 Bioanalyzer. The RNA concentration was determined by Qubit RNA Assay Kit in Qubit 3.0 Flurometer (Life Technologies). RNA-seq libraries were sequenced on an Illumina NextSeq 550AR plantform with standard Illumina protocols at Annoroad Gene Technology Co., Ltd (Beijing, China). Three independent biological replicates were used per treatment.