Transcriptome sequencing and annotation of the predatory mite Metaseiulus occidentalis (Acari: Phytoseiidae): a cautionary tale about possible contamination by prey sequences
Total RNA was transcribed to cDNA with an oligo dT primer, with second strand then generated. The cDNA is digested with a restriction enzyme then adaptors ligated onto the double-stranded (ds) DNA.  The adaptor ligated cDNA fragments were the amplified by PCR. This product was purified and processed for emPCR and 454 sequencing according to the manufacturer's recommended methods.