GSM3692947: plain water [CRO232A1]; Mus musculus; RNA-Seq
Preparation of CD45 negative cells was achieved by MACS magnetic separation columns according to manufacturer specifications. CD45 negative cells were collected, pelleted by centrifugation, and resuspended in the appropriate amount of RLT buffer according to manufacturer instructions (Qiagen). RNA was isolated using the Qiagen RNeasy Micro Kit. Libraries were constructed from cDNA using the Ovation Ultralow System V2 (NuGEN Technologies).
GEO Accession
GSM3692947