iPSC-CP RNA: Sample fb1dafe4-ddc4-48d8-bb83-07780477e758
DNA and total RNA was extracted from frozen RLT plus pellets of 1x106 cells using the Qiagen AllPrep DNA/RNA Mini kit (Qiagen Cat# 80204) and eluted in molecular grade H2O. RNA concentration was measured by Nanodrop and RNA integrity was determined using the Agilent 2200 TapeStation System. Illumina Truseq Stranded mRNA libraries were prepared and sequenced by HiSeq2500, to an average of 20 M 100bp read-pairs.
alignment_software
STAR