16S Amplicon of Mouse Cecum Flora
We extracted genomic DNA from a small fraction of total cecal contents. We lysed samples in 100 킠 water with an equal volume phenol/chloroform and 100 킠 of 0.5mm glass beads. We subjected samples to five 1 minute vortexing cycles followed by1 minute on ice in between each cycle. We centrifuged samples for 15 minutes at 21,000rcf at 4캜, re-extracted, and ethanol precipitated them to yield purified genomic DNA.