GSM1526863: Intact 3; Mus musculus; RNA-Seq
RNA was extracted from intact, unsorted and MACS-sorted samples using the RNeasy micro kit (Qiagen). Each sample (each n) comprised material from 1, 2 or 8 mice in the intact, unsorted and MACS-sorted groups, respectively. RNA integrity was assessed on an Agilent2100 Bioanalyzer (Agilent, Santa Clara, CA) and the RNA integrity number of each sample was >8. PolyA-selected RNA was used for complimentary DNA library preparation using the NEBNext Ultra direction RNA Library Prep Kit according to manufacturer’s instructions (NEB, Ipswich, MA).  100 bp reads were sequenced on the Illumina HiSeq2500 platform in replicate flow cells.
GEO Accession
GSM1526863