RNA-seq of C. gattii
The complementary DNA (cDNA) library confection was performed with TruSeq  RNA Sample Preparation Kit v.2. The libraries were clustered with TruSeq  PE Cluster kit v.2, and quantified by real-time PCR using KAPA Library Quantification kit Illumina  platforms. The libraries were normalized to 4 nM and sequenced in a MiSeq sequencer, with MiSeq Reagent v3 (600 cycle)  in paired-end strategy (2x300).