GSM4503635: Delta-cell_enriched_2 [WS6_S6]; Mus musculus; RNA-Seq
Islets were dispersed, incubated with antibodies directed against surface markers, sorted by FACS as different subsets into lysis buffer and stored at -80°C until NGS For library construction 20 ng of high-quality (RIN >7) total RNA was processed using Ovation Solo RNA-seq kit (NuGEN, Leek, The Netherlands) per the manufacturer's instructions. Briefly, total RNA was treated with DNaseI and reverse-transcribed using random primers. Chemical treatment during second strand synthesis permits to achieve strand specificity. After end-repair, adaptors ligation and library amplification, depletion of rRNA is realized using AnyDeplete technology (NuGEN). Libraries were then quantified with Qubit HS DNA assay (ThermoFisher) and library profiles assessed using the DNA High Sensitivity LabChip kit on an Agilent 2100 Bioanalyzer (Agilent Technologies). Libraries were sequenced on an Illumina Nextseq 500 instrument using 75 base-lengths read V2 chemistry in a paired-end mode.
GEO Accession
GSM4503635