RNA-seq of TAIL7: IL7 24h stimulated
RNA was purified by TRIzol reagent according to the manufacturer's protocol. RNA was double purified using the Poly(A)Purist MAG Kit (Ambion) and mRNA-ONLY Eukariotic mRNA Isolation Kit (Illumina). Libraries were constructed with SOLiD Total RNA-seq Kit (Applied Byosystems).