WGS Illumina PE 350 bp from Neoascochyta sp. (HWLR27 strain)
HWLR27 was isolated from a Salix fragilis (Crack willow) leaf infected with Melampsora epitea (the causal agent of willow leaf rust). A M. epitea rust pustule had a small white growth on it and this was isolated to be a HWLR27 (haploid). HWLR27 was grown on potato dextrose agar and lysed with mortar and pestle using liquid nitrogen. The DNA was isolated using the PowerSoil DNA isolation kit (Mo Bio) and a minimum amount of 13.5 ug at 122 ng/uL, 260/280=1.88, 260/230=2.17 was obtained. Library preparation was completed using the Illumina TruSeq PCR-free kit with 350 bp insert, HiSeq v4 chemistry and 2x 125 bp paired-end reads and the sequence data was produced on an Illumina HiSeq 2500.