LAZ_SEA_Bv6- SEA_0176_20121008_Bv6
"PCR amplification of the SSU rRNA gene v6 region using the forward                 primers (967F) CTAACCGANGAACCTYACC, CNACGCGAAGAACCTTANC, ?CAACGCGMARAACCTTACC,                 ATACGCGARGAACCTTACC, and Reverse Primer (1064R)                 ?CGACRRCCATGCANCACCT" 
nucl_acid_ext
Gentra Puregene Yeast/Bac Kit
lib_reads_seqd
860659
lib_const_meth
Fusion 2-step amplification
target_gene
16S rRNA
target_subfragment
v6
pcr_primers
Forward Primers (967F) CTAACCGANGAACCTYACC CNACGCGAAGAACCTTANC                     ?CAACGCGMARAACCTTACC ATACGCGARGAACCTTACC Reverse Primer (1064R)                     ?CGACRRCCATGCANCACCT
pcr_cond
1st step: 95°C for 3 min, 20x(95°C for 30 sec, 60°C for 45 sec and 72°C for                     45 sec), 72°C for 5 min. 2nd step: 95°C for 3 min, 10x(95°C for 30 sec, 60°C for                     45 sec and 72°C for 45 sec) 72°C for 5 min.
seq_quality_check
PMID: 23799126
url
http://vamps.mbl.edu/project_pages/ppindex.php?project_code=LAZ_SEA