16S barcoded sequences
50 samples total were pooled into two parts, separated as 'runs' in this entry.  Each run was separated into different lanes of a 454 plate for convenience in sequencing, and thus the files in each 'run' can be extracted and then combined prior to demultiplexing.  This will produce two separate runs.  The first 24 samples are in the 'run 1' and samples 25-50 are in 'run 2'.  The same bar-codes were used for separate samples between the two runs, so they must be demultiplexed as two separate runs.  Following demultiplexing, the final sequence files can be combined and thus will comprise 50 individual samples.