GSM1412547: PAO1_Neg_Cntrl_1; Pseudomonas aeruginosa; RNA-Seq
Cells intended for RNA sequencing were rapidly suspended in 1/10th volumes of ice cold stop solution (10% phenol in ethanol) and chilled to inhibit RNA transcription and degradation at indicated time points.  Cells were then collected by centrifugation (6,000 x g) and Total RNA was extracted from the resulting cell pellet by vigorously resuspending it in TRIzol (Ambion) and performing a classical phenol chloroform extraction followed by ethanol precipitation.  Remaining DNA was removed using TURBO DNAse (Ambion), the efficiency of which was checked by the absence of reporter PCR products of both bacterial and phage genes. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM1412547