GSM4678021: sw620-vector; Homo sapiens; Bisulfite-Seq
Cultured cells were washed on ice with ice-cold PBS, detached using cell scrapers and collected by centrifugation. Nucleic acids were subsequently extracted using the Promega Wizard Genomic DNA Purification kit according to instructions and stored at -80°C until further processing. DNA libraries were prepared using methylated adapters and the NEBNext DNA library prep master mix set following manufacturer recommendations. Libraries were bisulfite-converted using the Imprint DNA modification kit , and PCR amplified for 12 cycles using barcoded primers according to manufacturers instructions.
GEO Accession
GSM4678021