miRNAseq for human colon cancer
Indexed small RNA sequencing libraries were prepared from the RNA samples using the TruSeq Small Total RNA Sample Prep Kit, and bar-coded with individual tags. Library preparation was performed similarly to the WXS sequencing. Quality control was performed at every step, and the libraries were quantified using the Agilent 2100 Bioanalyzer. Indexed libraries were prepared as equimolar pools and loaded on the NextSeq500 (1x75 single read) run to generate at least 5,000,000 single reads per sample library. The raw Illumina sequence data were demultiplexed and converted to fastq files.