GSM3168374: lysate dilute no_drug_NO_DMS, DMSMaP-seq; Saccharomyces cerevisiae; RNA-Seq
RNA was extracted using SDS/hot phenol/chloroform. Fragments ranging from 60-80 nt were gel purified. Upon dephosphorylation of sample RNA, linker was ligated and this product was once again gel purified. This was followed by reverse transcription as well as circularization of cDNAs. PCR amplification of this library was then sent for sequencing.
GEO Accession
GSM3168374