GSM3168503: RNA-seq WT 2 SB2h; Mus musculus; RNA-Seq
Total RNA purified from d2.5 EBs with Trim33 WT ESCs in C57Bl/6J background (as described (Xi et al., 2008) was quantified by Ribogreen and quality assessed by Agilent BioAnalyzer 2000 The RNA-seq library preparation and sequencing was performed as descripted before (Wang et al.,2017) After scraped from plate, embryonic stem cells were washed with PBS and incubated in lysis buffer.
GEO Accession
GSM3168503