GSM1526565: at-seed-ler_wt_x_col_wt_1; Arabidopsis thaliana; RNA-Seq
For RNA sequencing, seeds from 20 siliques of Ler x Col, Ler x osd1, Ler x adm osd1, Ler x suvh7 osd1, and Ler x peg2 osd1 were harvested at 6 DAP into RNA later (Sigma-Aldrich) in duplicates and homogenized (Silamat S5; IvoclarVivadent) using glass beads (1.25-1.55 mm; Carl Roth). RNA was extracted following a modified protocol for the RNAqueous kit (Ambion, Life Technologies). RNA was purified by Qiagen RNeasy Plant Mini Kit (Qiagen) after residual DNA removed by 2 uL DNaseI (Thermo-Scientific). The library was prepared using the Truseq RNA Sample Preparation Kit (Illumina)
GEO Accession
GSM1526565