HiSeq2000 mRNA sequencing for pool of 6 reverted nurse brains (3 of 6)
RNA samples for the 6 pools of 6 brains of reverted nurse and foragers were derived from the same lysate that was used to create the reverted nurse and forager WGBS libraries. RNAseq libraries were created using the Illumina TruSeq RNA sample prep kit with no modifications to the standard protocol. This kit enriches for mRNA by using beads bound with a poly-T oligo to bind to the poly-A tails of mRNA.