WGS of P. aeruginosa ipcd479
Genomic DNA (250-750 ng) was mechanically fragmented for 40 seconds using a Covaris M220 (Covaris, Woburn MA, USA) with default settings. Fragmented DNA was transferred to a PCR tube and library synthesis was performed with the NEBNext Ultra II DNA library prep kit for Illumina (New England Biolabs, Ipswich MA, USA) according to manufacturers instructions. TruSeq HT adapters (Illumina, SanDiego CA, USA) were used to barcode the libraries, which were each sequenced in 1/48 of an Illumina MiSeq 300 bp paired-end run at the Plateforme dAnalyses Gnomiques of the Institut de Biologie Intgrative et des Systmes (IBIS, Universit Laval, Quebec, Canada)