CTCF binding evolution in mammals
CTCF binding evolution in mammals
The protocol describing the mouse strain TC1 can be found at http://dx.doi.org/10.1126/science.1114535. For the TC1 analysis the obtained reads were aligned to the entire mouse genome + an additional human chromsome 21 and regions of known deletions and insertions in this chromosome were removed. Hepatocytes were prepared by direct perfusion of the liver with buffered salt solution followed by percent formaldehyde. After 10 minutes the tissue was removed and diced in 500 mM glycine buffer to neutralize the formaldehyde. After homogenization the hepatocytes were rinsed with PBS.
Experimental Factor: IMMUNOPRECIPITATE
input_DNA
Experimental Factor: ORGANISM
TC1 mouse