NextSeq of Pseudomonas fluroescens SBW25: serCGA gene deletion compensated by experimental evolution, deletion mutant 2, evolution Line M3, isolate 1 (M3-L)
The isolate was purified from an agar plate of the Day 13 population by streaking to purification on fresh KB agar. A single colony was grown up in KB culture and stored as a glycerol stock at -80C. A scraping of the glycerol stock was used to start an overnight culture in liquid KB (28C, shaking, ~16 hours). 1 ml of culture was used to isolate genomic DNA with the Qiagen Blood and Tissue extraction kit (following manufacturer's instructions). DNA quality was checked on an agarose gel and Nanodrop. The library was prepared for Illumina paired end NextSeq sequencing (2x150 bp).