RNA-Seq profiling of the murine heart transcriptome
Index-tagged cDNA libraries were generated from total RNA using the TruSeq RNA Sample Preparation v2 Kit (Illumina, San Diego, CA). The quality, quantity and size distribution of the Illumina libraries were determined using the DNA-1000 Kit (Agilent Bioanalyzer). Libraries were sequenced on the Illumina Genome Analyzer IIx following the standard RNA sequencing protocol with the TruSeq SBS Kit v5. FASTQ files containing the reads for each library were extracted and demultiplexed using Casava v1.8.2 pipeline.