Illumina Genome Analyzer IIx sequencing of SAMD00024374
MDA-MB231 cells were cultured in Leibovitz's L15 (Wako, Osaka, Japan) medium supplemented with 10% FCS, under 100% air without CO2, and transfected with LacZ (control) vectors using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's protocol. The cells were lysed in Trizol reagent (Invitrogen) and total RNA was extracted according to the manufacturer's protocol. Sequencing sample libraries were prepared using a TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA, USA).  