GSM2577305: Owacja_D10_2; Solanum tuberosum; RNA-Seq
During the drought experiment, leaves-samples were taken everyday from three different plants for each variety at given time point, and immediately frozen in liquid nitrogen for storage and further use. Third, fourth and fifth whirl from the top of the plant complex leaves were taken from each plant. Total RNA was extracted from plant tissue using TRIzol and chloroform reagents followed by ethanol precipitation. Quality of RNA samples was tested with the use of Agilent RNA 6000 Nano Kit Guide (Agilent Technologies), only samples with: 28S:18S > 1.0, RIN≥ 6.5, OD 260/280 ≥1.8 and OD 260/230 ≥ 1.8 were used. Strand-specific library constructions and RNA-seq (PE100) using Illumina HiSeq 2500 were conducted by BGI Tech Solutions Co. Ltd. (Hong Kong).
GEO Accession
GSM2577305