Oryctes borbonicus transcriptome
Beetle legs were frozen in liquid nitrogen and ground to a fine powder using mortar and pestle. The powder was directly transferred into Tri Reagent® (Sigma-Aldrich, Munich, Germany) and RNA was extracted with the Direct-zolTM RNA MiniPrep (Zymo, Freiburg, Germany) following the manufacturer’s protocol. RNA quality was determined with a NanoDrop ND 1000 spectrometer (PeqLab, Erlangen, Germany) and RNA integrity was checked with the Agilent RNA Nano Chip Assay (Agilent, Santa Clara, USA). Only RNA samples of high quality (OD 260/280 > 2 and OD 260/230 > 1.8) and high integrity were used for library preparation.  High quality RNA was used to prepare two libraries following Illumina’s true seqRNA-sequencing protocol (Illumina, Eindhoven, Netherlands).