GSM3936264: 4.5µM NAA rep3; Arabidopsis thaliana; RNA-Seq
Rosettes were carefully separated from roots, immediately flash frozen in liquid nitrogen and stored at -80°C until further analysis. RNA was harvested using Spectrum™ Plant Total RNA kit (STRN250,Sigma, NSW, Australia) following manufacturer's guidelines. Illumina TruSeq stranded mRNA library kit (Catalog # RS-122-9004) was used for construction of sequencing libraries. Libraries were prepared using standard protocol by TruSeq stranded mRNA library kit (Illumina, Catalog # RS-122-9004)