RNA-seq of rice nd
Message RNA (mRNA) was enriched from 4 ug total RNA by using magnetic beads linked with oligo dT. The obtained mRNA was fragmented into short fragments and used to the first-strand cDNA synthesis and second strand cDNA synthesise. The resulting double-strands cDNA was purified by AMPure XP beads. Purified double-strands cDNA were then used to end repair, 3' end adenosine tailing, sequencing adaptor connection, fragment selection and finally PCR amplification to generate sequencing library. The library quality was evaluated by using the Agilent Bioanalyzer 2100 system (Agilent, Santa Clara, CA 95051, USA) and ABI Step-One-Plus real-time PCR system.