GSM1528610: compound C3 replicate 2; Homo sapiens; RNA-Seq
RNA was purified with the RNeasy Mini Kit (Qiagen) Template DNA molecules suitable for cluster generation were prepared from 1 ug of total RNA samples using the TruSeq RNA Sample Preparation Kit v2. Libraries were quantified using the KAPA Library Quantification Kit (KK4824, Kapa Biosystems, Boston, MA). The libraries were pooled at equimolar concentrations and diluted prior to loading onto the flow cell of the HiSeq 2500 (Illumina Inc., San Diego, CA) for both clustering and sequencing. The libraries were extended and bridge-amplified to create single sequence clusters using the TruSeq Rapid PE Cluster Kit - HS (Illumina Inc., San Diego, CA). Amplified clusters in the flow cell were then sequenced with 50-bp paired-end reads using the TruSeq Rapid SBS Kit - HS (Illumina Inc., San Diego, CA) in rapid run mode.
GEO Accession
GSM1528610