transcriptome
For mRNA library construction, mRNA in the sample was enriched, fragmented. The RNA fragments were served as a template for first strand cDNA synthesis using random hexamer-primers. The second strand cDNA was synthesized using buffer, dNTPs, RNase H and DNA polymerase I. The double strand cDNA were purified and resolved with EB buffer for end reparation and poly(A) addition. The fragments were then ligated with sequencing adapters. After agarose gel electrophoresis, cDNA fragments with a desired length were obtained and amplified by PCR to construct the final cDNA library for paired-end sequencing.