GSM1959697: Sample_KP28; Danio rerio; RNA-Seq
Ventricles were collected from adult tcf21:nucEGFP fish at 6 months of age. Ventricles were collected on ice and washed several times to remove blood cells.  Ventricles were digested in an Eppendorf tube with 0.5 ml HBSS plus 0.13 U/ml Liberase DH (Roche) and 1% sheep serum at 37°C, while stirring gently with a Spinbar® magnetic stirring bar (Bel-Art Products).  Supernatants were collected every 5 min and neutralized with sheep serum.  Dissociated cells were spun down and re-suspended in DMEM plus 10% fetal bovine serum (FBS) medium with 1.5 μg/ml propidium iodide (PI), and sorted using a BD FACSVantage SE sorter for EGFP-positive and PI-negative cells.  Single cells were captured using Fluidigm C1 platform. RNA extraction was done following the Fluidigm protocol. Libraries were constructed on the C1 platform following the Fluidigm protocol.
GEO Accession
GSM1959697