GSM4565551: ESC_WT_Input2h_rep1; Mus musculus; OTHER
Edu-seq was performed as described (Macheret and Halazonetis 2019). Briefly, cell were fixed in 90% cold methanol and permeabilized with 0.2% Triton X-100/PBS for 30min. Edu-labeling cells were incubated with Click-it reaction mix (ThermoFisher, C10269). DNA was then sheared to 150-500 bp. Edu-labeling DNA was isolated and used for Edu-seq according to reference. Libraries were constructed according to the Illumina Tru-seq protocol.
GEO Accession
GSM4565551