GSM3936852: Sample1_Ctrl_Slam_3; Mus musculus; RNA-Seq
Cells were sorted in pools of 500 cells into 25uL Qiagen RLT + 1%beta-mercaptoethanol (v/v) and frozen at -80C Lysates were converted to cDNA following capture with Agencourt RNA Clean beads using the SmartSeq2 protocol as previously described (Trombetta et al, Curr Protoc Mol Biol, 2014)