Illumina HiSeq 2000 paired end sequencing; Computational assignment of cell-cycle stage from single-cell transcriptome data
Computational assignment of cell-cycle stage from single-cell transcriptome data
2i+LIF conditions (1M PD0325901 (Stemgent), 3M CHIR99021 (Stemgent), 100 U/ml recombinant human leukemia inhibitory factor (Millipore)). Asynchronous mES cells were stained with DNA dye (Hoechst) and sorted for cell cycle stage based on DNA content (2n-G1 stage, 2n:4n-S stage and 4n-G2M stage) Bulk cells were lysed as per Wellcome Trust Sample preparation guidelines Bulk mRNA sequencing libraries were generated using Illumina TruSeq Stranded RNA Sample preparation kit as per Wellcome Trust Sample preparation guidelines
Experimental Factor: S
cell cycle