16S-Seq of cherry metagenome: RR
Bacterial 16S rRNA gene V3-V4 hypervariable regions (forward primer ACTCCTACGGGAGGCAGCA; reverse primer GGACTACHVGGGTWTCTAAT) were PCR-amplified. Amplicon sequencing libraries were constructed using a TruSeq Nano DNA LT Library Prep Kit for Illumina (NEB, USA) according to the manufacturer's instructions, while index codes were added.