3-5 kb insert mate pair library
The mate-pair libraries were constructed with the Nextera Mate Pair library Sample Prep kit (Illumina, CA), followed by the TruSeq DNA Sample Prep kit.  The libraries were quantitated by qPCR and sequenced on one lane for 161 cycles from each end of the fragments (resulting in 150 bp reads) on a HiSeq 2500 using a HiSeq Rapid SBS sequencing kit version 2. Fastq files were generated and demultiplexed with with the bcl2fastq v2.17.1.14 Conversion Software (Illumina).