GSM3937190: Bone marrow cells, P210XB7; Mus musculus; RNA-Seq
Fresh bone marrow cells were isolated by crushing mouse bones in media supplemented with FCS and the supernatant was collected. Cells were stained with fluorescently labelled antibodies (CD45, Ter119, CD41, CD71, CD51, CD61, CD200, VCAM1) . A vibrant dye was used to exclude debris. Single cells were sorted into 96-well-plates in Smart-seq2 lysis buffer. Full-length cDNA from individual cells were produced following the SmartSeq2 protocol. Tagmentation using Homemade Tn5 (Hennig et al., G3 2018)