Lake Erie Metatranscriptome Sample 973 July B
RNA was extracted using the MoBio DNA isolation kit for Sterivex modified for RNA. To optimize the protocol, sterivex were vortexed for 5 minutes longer than recommended each time and all wash buffers were allowed to sit for one minute before being pulled through the binding column using a vacuum manifold. DNase treatment was performed as recommended in the protocol using the MoBio On-spin Column DNase kit. This protocol was optimized by allowing the DNase solution to sit for an extra 15 minutes than recommended. RNA was checked for DNA contamination using universal 16S primers (27F and 1522R). Any additional DNase treatments needed were performed using Ambion's Turbo DNase kit. RNA was stored at -80şC until sent to Hudson Alpha for sequencing  .