GSM3936390: Farber_452; Mus musculus; RNA-Seq
Total RNA was extracted from the mineralized cultures using mirVana RNA isolation kit (ThermoFisher Scientific). RNA-Seq libraries were constructed from 200 ng of total RNA using Illumina TruSeq Stranded Total RNA with Ribo-Zero Gold sample prep kits (Illumina, Carlsbad, CA). Constructed libraries contained RNAs >200 nt (both unpolyadenylated and polyadenylated) and were depleted of cytoplasmic and mitochondrial rRNAs.