GSM4279940: PANX1 rep 2 [treatment_1]; Homo sapiens; RNA-Seq
Total RNA was extracted using RNeasy Mini Kit (Qiagen, Germantown, MD, USA). Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 500 ng of total RNA for the construction of sequencing libraries. RNA was purified first for Poly A tail containing mRNA molecules using poly-T oligo attached magnetic beads.The purified RNA was fragmented on thermocycler at 94 C for 8 min in fragmentation buffer. The cleaved RNA fragments were copied into first strand cDNA using reverse transcriptase and random primers. This was followed by second strand cDNA synthesis using RNase H and DNA Polymerase I. A single “A” base was added and adapter ligated. The cDNA was purified and then enriched by PCR to create cDNA libraries.
GEO Accession
GSM4279940