GSM2579124: MRC5 Control Replicate 1 [miRNA-seq]; Homo sapiens; miRNA-Seq
Total RNAs were extracted from MRC5 cells using TRIzol reagent (Invitrogen). 500ng of total RNA were ligated, reverse transcribed and amplified (18 cycles) with the reagents from the NextFlex small RNAseq kit V3 (Bioo Scientific). Amplified libraries were quantified with the Bioanalyzer High Sensitivity DNA Kit (Agilent), pooled and size-selected from 140 nt to 170 nt with the LabChip XT DNA 300 Assay Kit (Caliper Lifesciences). Libraries were then sequenced on an Illumina Nextseq 500 Mid Flowcell with 75bp reads.
GEO Accession
GSM2579124