Next Generation RNA Sequencing reveals defects in nuclear mRNA maturation of Arabidopsis thaliana lefko2 mutant
Total RNA from lefko2 mutant cotyledons was extracted using the NucleoSpin Plant RNA kit (Macherey-Nagel, Düren, Germany) with an on-column DNase treatment according to the manufacturer's instructions. The quantity and integrity of the RNA were assessed using a NanoDrop 1000 spectrophotometer and agarose gel electrophoresis. RNA-seq libraries were generated using the TruSeq Low Input kit according to the manufacturer's instructions (Illumina). RNA seq on an Illumina HiSeq 2500 instrument at BGI (Beijing Genomics Institute).