GSM4626587: O RNA-Seq replicate 3; Mus musculus; RNA-Seq
FACS-isolated MuSCs were snap-frozen. ERCC RNA (Invitrogen 4456740) was spiked in proportional to cell number: 10 mcL of 1:10,000 diluted ERCC RNA per 122,000 cells. RNA was extracted using the Nucleospin RNA XS kit (Machery-Nagel). RNA (10 ng) was reverse-transcribed using oligo(dT) priming with the SMARTer Ultra Low Input system (Takara). The cDNA then sheared with a Covaris S2 ultrasonicator. End repair, multiplexed adapter ligation, and 6-9 cycles of library amplification were performed using the Ovation Ultralow Multiplex system (NuGEN). Libraries underwent paired-end 101-bp sequencing at the Stanford Genome Sequencing Service Center with an Illumina HiSeq 2000 to a depth of 26-37 million reads.
GEO Accession
GSM4626587