European D. pulex genome
Genomic DNA was purified using Plant Genomic DNA Purification Mini Kit (Thermo Scientific, protocol A) following manufacturer’s instructions. DNA elution was performed twice, both times using 70 µl and 50 µl 70 °C elution buffer. DNA from parthenogenetic D. pulex adults was used. 100 ng of extracted DNA was fragmented using MuSeek Library Preparation Kit, Illumina compatible (Thermo Scientific) and purified using GeneJET NGS Cleanup Kit (Thermo Scientific). The DNA was PCR amplified for 14 cycles and gel size selected for next generation sequencing.  The sequencing was performed using Illumina MiSeq platform with MiSeq reagent kit v3 600-cycle.