GSM1301656: Virus-free; Malus domestica; RNA-Seq
Two RNA pools were obtained, separately, from 10 ASGV-infected plantlets and 10 virus-free plantlets cultured under the same growth conditions. Total RNA was extracted from mixed samples according to the method described by Gambino et al., Phytochemical Analysis, 2008. After treatment with DNase I and extraction with phenol/chloroform and precipitation, RNA pellets were dissolved in 30 μL of RNase-free water and visualized in a 1.0% agarose gel and quantified using a NanoDrop ND-2000 Spectrophotometer (NanoDrop Technologies, Wilmington, Delaware, USA). RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM1301656