GSM4566746: DU145 control rep 3; Homo sapiens; RNA-Seq
Total RNA was purified from LNCaP and DU-145 cells with miRVana Isolation Kit (Thermo Fisher Scientific, Waltham, MA, USA) according to manufacturer's instruction. Briefly, samples were lysed in a denaturing lysis solution, and RNA was extracted using Acid-Phenol:Chloroform and fibre-glass filter cartridges. Total RNA was eluted with a low ionic-strength solution provided with the kit. RNA concentration, purity, and integrity were assessed by Qubit (Invitrogen, USA) and Tape Station 2200 (Agilent Technologies, USA). RNA-seq libraries were constructed from 1 µg of total RNA with RNA integrity number (RIN) > 8, and using the Illumina TruSeq® Stranded Total RNA Library Prep Kit (#20020598, Illumina, USA). Indexed libraries were pooled, clustered with use the cBot and sequenced on the Illumina HiSeq 4000 platform generating 150 bp paired end reads (2 x 75 bp).
GEO Accession
GSM4566746