Sample 2 from liquid part of grass and pig manure co-digestion
Sample "Biograss_Liquid_Fraction_LG2" was collected from steady state reactors digesting swine manure and pretreated ensiled meadow grass. Approximately 3 g of wet grass and fibers with firmly adherent bacteria were separated from the liquid phase using a sterile net and washed intensively with cold sterile BA medium to remove unattached and loosely adherent microbes. Plant material was removed from samples of liquid phase samples (LG) by filtering with a 40-μm nylon cell strainer filter and by centrifugation (500 x g for 15 min). Supernatant was centrifuged for 20 min, 10000 g at 4°C and resultant cell pellet was processed to extract genomic DNA according to PowerSoil® DNA Isolation Kit protocol. DNA libraries were prepared with Nextera XT kit (Illumina, San Diego, CA) and samples were sequenced using NextSeq 500 sequencing technology producing 150 bp paired end reads.