GSM3169354: Gcn5AA_Rapa_lab_RNA; Saccharomyces cerevisiae; RNA-Seq
Before extraction, previously 4-tU labeled S.pombe cells were mixed to S.cerevisiae cells to a ratio of 1:6. RNA was extracted with the standard hot phenol method and treated with DNAse I for 15' at room temperature. 4-tU labeled RNA was purified as described in (Baptista, T. 2017; PMID: 28918903) TruSeq stranded mRNA kit (Illumina)
GEO Accession
GSM3169354