16S rRNA sequencing of maize seminal and crown root defective mutant rtcs rhizosphere: zone2 of primary root
Different maize genotypes were sown in the phytochamber with low nitrogen natural soil for 4 weeks and then rhizosphere soil from the primary root were dissected into three zones corresponding to root developmental zones. The genomic DNA was isolated from those rhizosphere soil and subjected to 16S rRNA gene V4 sequencing.