GSM1960244: dat-Medial-1 midbrain dopamine neurons; Mus musculus; RNA-Seq
First strand cDNA was initially amplified by long-distance PCR (LD-PCR) using hot-start amplification. 2-7 ng cDNA was sonicated (Covaris S2; Covaris Inc., Woburn, MA, USA) and  ~200 bp fragments end-repaired following adapter ligation, and enriched with 10 cycles of PCR for HiSeq 2500 technology (Ilumina Inc., San Diego, CA) using TruSeq DNA Sample kit (FC-121-2001, Illumina) following manufacturer`s instructions, and sequenced as 100 bp single reads at the RU Genomics Resource Center. The quality and quantity of the libraries were validated using Agilent’s High Sensitivity DNA Chip (Agilent) for the Agilent 2100 Bioanalyzer
GEO Accession
GSM1960244