Whole genome sequencing of the  Arcobacter acticola type strain KCTC 52212
Illumina  libraries were prepared for all strains using the KAPA Low-Throughput Library  Preparation Kit with Standard PCR Amplification Module (Kapa Biosystems,  Wilmington, MA), following manufacturer's instructions except for the  following changes: 750 ng DNA was sheared at 30 psi for 40 s and size  selected to 700-770 bp following Illumina protocols. Standard desalting  TruSeq LT and PCR Primers were ordered from Integrated DNA Technologies  (Coralville, IA) and used at 0.375 and 0.5 M final concentrations,  respectively. PCR was reduced to 3-5 cycles. Libraries were quantified using  the KAPA Library Quantification Kit (Kapa), except with 10 l volume and 90-s  annealing/extension PCR, then pooled and normalized to 4 nM. Pooled libraries  were requantified by ddPCR on a QX200 system (Bio-Rad), using the Illumina  TruSeq ddPCR Library Quantification Kit and following manufacturer's  protocols, except with an extended 2-min annealing/extension time. The  libraries were sequenced 2  250 bp paired end v2 on a MiSeq instrument  (Illumina) at 13.5 pM, following manufacturer's protocols.