Illumina HiSeq 2500 sequencing; Control and Bmi1-overexpressing adult murine neural stem cells
Control and Bmi1-overexpressing adult murine neural stem cells
Isolated neural stem cells from E18.5 or adult Swiss Webster mice were grown under non-adherent conditions for three weeks/two passages (eNSCs) or four weeks/three passages (aNSCs) prior to RNA isolation Embryonic NSCs were acutely transduced with empty vector (EV) or Bmi1-overexpressing lentiviral vector; adult NSCs were allowed to recover overnight prior to transduction Total RNA was isolated using RNAeasy Plus Micro kit (Qiagen) 150-250 ng RNA was used for library construction.  Ribosomal RNA was removed using the Ribo-Zero Magnetic Kit (Epicentre), and libraries prepared using the ScripSeq V2 RNA-seq Kit (Epicentre), using the low input protocol.
Experimental Factor: genetic modification
Bmi1-overexpressing lentiviral vector
Experimental Factor: developmental stage
embryonic day 18.5
LIBRARY_STRAND
not applicable