Sequencing of Mycobacterium tuberculosis: Human sputum
To perform a whole-genome sequence, we re-culture the isolates on Lwenstein-Jensen slant. The DNA was extracted according to the standard protocol for mycobacteria. Genomic libraries were constructed using Nextera XT DNA Sample Preparation Kit (Illumina) following the manufacturer's instructions. Individual libraries were indexed with Nextera XT Index Kit. Paired-end reads from all isolates were obtained using Illumina MiSeq platform at the National Institute of Infectious Diseases Dr. Carlos G. Malbran, Buenos Aires, Argentina.