nts382 leaf USDA110
The first trifoliate leaves were harvested from 3 soybean plants after 7 days inoculating with Bradyrhizobium diazoefficiens USDA115?wild-type or nodC- mutant strain. RNA was extracted by the Invitrogen PureLink RNA Mini Kit followed with mRNA isolation using magnetic oligo(dT) beads. The libraries were generated by Illumina TrueSeq kit according to manufacturer procedures.