RNA-seq of Aedes Aegypti: sRNA: sRNA construct related :4dpi: Midgut: 3
Mosquitoes (4-7 days old) were treated overnight with a feeding solution containing either HT27/OX (empty plasmid) or HT27/OXE (ptac-ds-eGFP). Midguts and carcasses were dissected in phosphate-buffered saline and pooled (n=10) at 1, 4, and 7 days-post-treatment (dpt). Total RNA was extracted using the MirVana miRNA Isolation kit (Thermofisher, AM1560), using linear polyacrylamide as a carrier (Thermofisher, AM9520).