GSM2417775: E11.5 Mouse NT PRDM13-Ab1 (ZF) ChIP; Mus musculus; ChIP-Seq
For full ChIP protocol see Meredith 2013 MCB 33:3166-3179. For extraction, nuclei were liberated from neural tube tissue by dounce homogenization in PBS and then fixed in 1% formaldehyde for 10 minutes at room temperature. Fixation was terminated by adding glycine to a final concentration of 0.125M. Chromatin was sheared using a Diagenode Bioruptor for 30 minutes on high power with 30s:30s on:off cycles. 100 μg chromatin was immunoprecipitated with 5 μg rabbit anti-PRDM13 antibody (Johnson Lab generated,  PA6659, rabbit anti-mouse PRDM13) followed by anti-mouse Dyna beads (Invitrogen). Libraries were made according to Illumina's ChIP-seq DNA sample prep protocol
GEO Accession
GSM2417775