C3xAlt_POOL2
Pooled multiplex fragment libraries of 46 individuals from a segregating F1 population generated from the cross of globe artichoke "C3" genotype and cultivated cardoon "Altilis 41" genotype. DNA samples were fragmented to lengths of ~300 bp by sonication using a Covaris E220 as per the manufacturer’s instructions (Covaris, Inc.). Genomic paired-end libraries were then prepared using standard procedures and NEB reagents for end-repair, A-tailing, and adapter ligation. Fragment size was controlled using Ampure beads purification procedure. Libraries were PCR-enriched using the high-fidelity Phusion polymerase with 5 cycles of amplification. These libraries were prepared with the aim to perform shallow coverage sequencing for genotype imputation purposes.  Individuals are identified with the following barcodes in the header: Art_7:CGATGT; Art_19:TGACCA; Art_36:ACAGTG; Art_64:GCCAAT; Art_82:CAGATC; Art_105:CTTGTA; Art_127:ATCACG; Art_148:TTAGGC; Art_8:ACTTGA; Art_20:GATCAG; Art_39:TAGCTT; Art_65:GGCTAC; Art_83:AGTCAA; Art_106:AGTTCC; Art_129:ATGTCA; Art_9:GTAGAG; Art_21:GTCCGC; Art_45:GTGAAA; Art_66:GTGGCC; Art_84:GTTTCG; Art_107:CGTACG; Art_132:GAGTGG; Art_10:ACTGAT; Art_22:ATGAGC; Art_46:ATTCCT; Art_67:CAAAAG; Art_88:CAACTA; Art_109:CACCGG; Art_134:CACGAT; Art_38:CACTCA; Art_26:CAGGCG; Art_23:CATGGC; Art_48:CATTTT; Art_68:CCAACA; Art_89:CGGAAT; Art_110:CTAGCT; Art_135:CTATAC; Art_41:CTCAGA; Art_12:GCGCTA; Art_24:TAATCG; Art_52:TACAGC; Art_72:TATAAT; Art_90:TCATTC; Art_111:TCCCGA; Art_137:TCGAAG; Art_62:TCGGCA