ECR11_Rep1 RNA-derived tag sequencing: aliquot 20
       Library of tagged enhancer haplotypes was introduced into mouse liver via hydrodynamic tail-vein injection. Total RNA was extracted after 24h, treated with DNase I and split into aliquots (~1ug of RNA per aliquot). Each aliquot was subjected to RT-PCR, seeking to amplify the unique tag associated with each enhancer haplotype. The primers used for the RT-PCR contained the necessary sequences for compatibility with the Illumina flow-cell. Thus, the cDNA library obtained at the end of this step was ready for sequencing, eliminating the need for a separate sequencing-library construction step.        RT-PCR and sequencing of tags from aliquots of RNA extracted from mouse liver.       