Illumina HiSeq 1500 sequencing; A genomewide map of PPAR_/_ target genes in human macrophages identifies a unique agonist-induced activation state - RNAseq
A genomewide map of PPAR_/_ target genes in human macrophages identifies a unique agonist-induced activation state - RNAseq
Cells were isolated on June 17th 2014 from healthy human donor blood of unknown gender via LSM1077 gradient centrifugation. Adherent cells were positivly selected on primaria 6 well dishes (3x10^6 monocytes per well in 2 ml PBS (PAA)) followed by 6 washing steps with PBS. Finally the monocytes were cultured for 7d in humidified incubator at 37 oC, 5 % CO2 in 2ml X-VIVO 10 (Biozym scientific, 881022),  medium. Cells were treated with control (DMSO), L165 1 uM final concentration or ST247 300 nM final concentration for 1d. Cells were lysed in Peqgold (TRIzol-like solution based on phenol and chloroform). RNA was digested with DNase (MN) and purified on a Qiagen Minelute column. Illumina-compatible libraries were made using the Epicentre Scriptseq human/mouse/rat low input Gold kit.
Experimental Factor: growth condition
XVIVO
Experimental Factor: compound
PTS264
Experimental Factor: dose
1 micromolar
LIBRARY_STRAND
first strand