GSM3097070: CD4+CCR7-CD38- (Non Activated) From Volunteer T4 (Beta); Homo sapiens; OTHER
Upon FACS sorting, RNA was extracted using RNAqueous-Micro Total RNA Isolation Kit (Ambion), following the manufacturer's instructions. RNA was reversed transcribed using a template-switch oligo with a 6bp unique molecular identifier and primers designed for the constant regions of Trac and Trbc genes. TCR amplification was achieved by performing two rounds of nested PCR using primers specific for the constant region of the TCRs. A final PCR was performed to add the Illumina adaptors. Libraries were sequenced on Illumina MiSeq. The R1 reads contain the unique molecular identifier (UMI) tag (atcaacgcagagtacatggNNNNNNgg).
GEO Accession
GSM3097070