iPSC RNA: Sample 9dbe1e08-cc7f-4269-83b3-7d77d886b14c
Total RNA was extracted from 222 iPSC lines using AllPrep DNA/RNA Mini Kit (QIAGEN) following the manufacturer's protocol. RNA quality was assessed based on RNA integrity number (RIN) using an Agilent Bioanalyzer. Any samples with RIN less than 7.5 were re-isolated. Libraries were prepared using the Illumina TruSeq stranded mRNA kit and sequenced using an Illumina HiSeq2500 (∼11 samples per lane). Samples were sequenced to an average of ∼22 million read pairs. Biological replicates were sequenced for some lines.
alignment_software
STAR