GSM3554071: Nintedanib_2uM_IPF; Homo sapiens; RNA-Seq
total RNAs from Ctrl_IPF fibroblasts, Nintedanib_2uM_IPF fibroblasts and Nintedanib_4uM_IPF fibroblasts were extracted by Trizol Reagent according to the manufacturer's instructions. All RNA Sample preparation procedures were carried out according to the official Illumina protocol.  Agilent's SureSelect Strand-Specific RNA Library Preparation Kitwere used for library construction followed by AMPure XP Beads size selection.  The sequence was directly determined using Illumina's sequencing-by-synthesis (SBS) technology. Sequencing data (FASTQ files) were generated by Welgene's pipeline based on Illumina's basecalling program bcl2fastq v2.2.0.
GEO Accession
GSM3554071