GSM2413579: Mouse_Hepatocyte_RNA_DMHCA-2_EDM_15-07-30; Mus musculus; RNA-Seq
RNA was isolated using Trizol and Direct-zol columns (Zymo) PolyA mRNA was selected from total RNA prior to fragmentation, first strand and second strand synthesis incorporating deoxy-UTP. Following dA-tailing and barcode adapter ligation the product was PCR amplified and subsequently size-selected (223-375 bp) using TBE gel electrophoresis.  The excised library was purified prior to sequencing.
GEO Accession
GSM2413579