GSM3693030: HERMES; Homo sapiens; RNA-Seq
Cells were grown to 80% confluence and harvested at room-temperature using the RNeasy mini kit (QIAGEN). Cells were scraped in PBS and lysed in Buffer RLT supplemented with 143 mM β-mercaptoethanol. The lysate was homogenised by being passed 10 times through a 0.9 mm needle and RNA purified on RNeasy spin columns, on-column DNase digestion was performed at room temperature for 15 minutes before being eluted in nuclease free water. Library preparation and paired-end sequencing (RNA-Seq) was performed byHigh Throughput Genomics, Wellcome Trust Centre for Human Genetics, University of Oxford. A minimum depth of 40M mapped reads were generated per sample
GEO Accession
GSM3693030