GSM3167408: HeLa, MeV-CKO(GFP)-infected (MOI=3, 24 h); Homo sapiens; RNA-Seq
Trizol RNA extraction, isopropanol precipitation, rRNA depletion w/ Ribominus Kit (Life Technologies) RNA libraries were prepared using 100 ng of total RNA according to the manufacturer's instructions for the TruSeq Stranded Total RNA Sample Prep Kit (Illumina, San Diego, CA, Cat# RS-122-2201).  The concentration and size distribution of the completed libraries was determined using an Agilent Bioanalyzer DNA 1000 chip (Santa Clara, CA) and Qubit fluorometry (Invitrogen, Carlsbad, CA). Libraries were sequenced at 60-110 million reads per sample following Illumina's standard protocol using the Illumina cBot and HiSeq 3000/4000 PE Cluster Kit (Illumina, Cat# PE-410-1001). The flow cells were sequenced as 100 X 2 paired end reads on an Illumina HiSeq 4000 using HiSeq 3000/4000 sequencing kit (Illumina, Cat# FC-410-1002 and FC-410-1001) and HCS v 3.3.52 collection software. Base-calling is performed using Illumina's RTA version 2.7.3.
GEO Accession
GSM3167408