GSM4565567: HepG2-p53-mRNA-seq rep 1; Homo sapiens; RNA-Seq
Total RNA was extracted from the cultured cells using Trizol Reagent (Invitrogen) according to the manufacturer's instructions.mRNA was purified from total RNA using poly-T oligo-attached magnetic beads, and double stranded cDNA synthesized using random hexamers  cDNA was synthesized using random hexamers, M-MuLV Reverse Transcriptase (RNase H), and DNA Polymerase I.The library fragments are purified with AMPure XP system RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM4565567