NGS of fecal sample of F344 rat: 74w.Female.HFD.3
V3-V4 region of 16S rRNA was amplified with PCR. The primers were 341F and 848R. PCR products were confirmed with electrophoresis and purified using QIAquick PCR purification kit (Qiagen, Valencia, CA, USA). The purified PCR products were tagged with Illu