GSM1468513: (II) N2A__rRNA_dep__Sfpq_KD; Mus musculus; RNA-Seq
Libraries were prepared according to Life Techonology's instructions accompanying the Ion AB Library Builder™ System and Library Builder. Briefly, total RNA was treated with RiboMinus™ Eukaryote System v2 (Life technologies, Carlsbad, CA, USA) to ribosomal RNA-depleted RNA. RNA libraries were then prepared using the Life Techonology Ion Total RNA-Seq Kit for the AB Library Builder™ System and Library Builder. High-throughput sequencing was performed using the Ion Proton System. Sfpq mRNA level was down-regulated less than 10% in Neuro-2a treated with Sfpq-siRNA comparing those of Luc-siRNA. Total RNA from Neuro-2a cells were extracted using RneasyMidi (Qiagen) according to the manufacturer's instructions.
GEO Accession
GSM1468513