HKU3 Coronavirus Transcriptome - Rhinolophys Sinicus attenuated
Goals: To define known and unknown virus-specific, virus-encoded transcripts present during infection with various coronaviruses. Setup and Design: Vero cells were infected with HKU3 coronavirus for 24 hours. Intracellular RNA was then harvested in TRIzol. RNA purified from TRIzol prep was subjected to RT-PCR and poly-A purification before generation of first - and second-strand cDNA and Illumina HiSeq analysis.