GSM1530044: H3K27me3_ChIP-seq_WT_Col/C24_Rep1; Arabidopsis thaliana; ChIP-Seq
Chromatin immunoprecipitation experiment was performed following previously published procedure (Gendrel et al. 2005). Briefly, 1.5 g of shoots of 14-day-old plants were cross-linked by 1% formaldehyde in a vacuum, and then the samples were ground to fine powder in liquid nitrogen. Chromatin was immunoprecipitated with antibody against H3K27me3 (Millipore).Total RNA was extracted from shoots of 14-day-old Arabidopsis seedling using the Rneasy plant mini kit (Qiagen). mRNAs were extracted from the total RNAs by Dynabeads oligo(dT) (Invitrogen Dynal). First- and second-strand cDNAs were obtained using random hexamer primers and SuperScript II reverse transcriptase (Invitrogen). DNA and RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM1530044