GSM3617727: syd-5_RNA rep2; Arabidopsis thaliana; RNA-Seq
Lysates were clarified from sonicated nuclei and the associated complexes were isolated with antibodies. 14-day-old seedlings were  frozen with liquid nitrogen. Total RNA was isolated from   ̴50 mg of in vitro-grown seedlings using the RNeasy Plant Mini kit (Qiagen) according to supplier's instructions. Total RNA was treated with RNase-free DNaseI kit (Life Techologies). 5 ng of ChIP DNAs were used for library construction per sample. Blunting end, adapter ligation and amplification were carried out using Illumina Genomic DNA Sample Prep Kits according to the manufacturer's protocol.
GEO Accession
GSM3617727