GSM2150394: Input wt1; Schizosaccharomyces pombe; ChIP-Seq
Cells were harvested and lysed with a FastPrep cell disrupter (FP120, Bio101 Thermo Savant). DNA was sheared to a range of 250-400bp with a Bioruptor Standard (Diagenode). Monoclonal anti-HA tag antibody [12CA5] (abcam16918) was used to retrieve HA-tagged Abp1 combined with Dynabeads Protein A (Life Technologies). After reversing the crosslinks DNA was purified using phenol-chloroform and precipitated with LiCl and isopropanol Libraries were prepared using Illumina’s ChIP-Seq Sample Prep Kit
GEO Accession
GSM2150394