GSM2417034: hiPSC-RPE 3 (Control 3) NAM; Homo sapiens; RNA-Seq
To extract RNA, hiPSC-RPE cells were scraped from the culture wells and collected in RNA protect. Total RNA was purified using the RNeasy plus micro kit (Qiagen) according to manufacturer's instructions. 10ng total RNA was used to generate libararies using the  SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian (Clontech) as per standard protocol. Final libraries are pooled at 1.6pM final concentration for sequencing (1x75 cycle) on an Illumina Nextseq500 instrument
GEO Accession
GSM2417034