GSM1528472: LH5-CJ48-Subst-Ind-50-CAATATC_S33_L001_R2_001.; Mus musculus; OTHER
DNA extraction was done as described in the following paper. Blondal, T., Thorisdottir, A., Unnsteinsdottir, U., Hjorleifsdottir, S., Aevarsson, A.,  Ernstsson, S., Fridjonsson, O.H., Skirnisdottir, S., Wheat, J.O., Hermannsdottir,  A.G., et al. (2005). Isolation and characterization of a thermostable RNA ligase 1  from a Thermus scotoductus bacteriophage TS2126 with good single-stranded  DNA ligation properties. Nucleic Acids Res 33, 135-142. Sequencing adaptors for Illumina MiSeq Sequencing was done by PCR as described for the HCoDES procedure in the reference above. Amplified PCR products were then pooled in apporxomately equimolar concentrations for Illumiina MiSeq using 40% Phi-X.
GEO Accession
GSM1528472