Estrogen primed, progesterone treated cells immunoprecipitated with PR antibody
12,000,000 ZR-75-1 cells were plated in phenol red free RPMI1640 supplemented with 10% hormone stripped FBS and 10nM 17beta-estradiol. After 72h, cells were treated with 10nM progesterone for 4 hours before fixation of the cells and immunoprecipitation with PR H190 antibody. Four indpendently performed ChIP reactions were performed, validated with quantitative RT-PCR and then pooled for a single library prep using standard Illumina protocols.