Illumina HiSeq 4000 paired end sequencing; Whole exome sequencing of Rb1 and Trp53 deficient (RP) SCLC mouse model
Whole exome sequencing of Rb1 and Trp53 deficient (RP) SCLC mouse model
Cell pellets collected under steady state growth conditions Genomic DNA was extracted with the Gentra Puregene Tissue Kit (Qiagen) according to the manufacturerâ€™s instructions Library preparation was performed using the SureSelectXT Library Prep Kit followed by Target Enrichment Kit using the Mouse All Exon Capture ab (Agilent, USA).
Experimental Factor: genotype
Trp53/Rb1-deficient SCLC model