GSM3936208: UT_4; Homo sapiens; OTHER
Genomic DNA was isolated from frozen tissues by TIANamp Genomic DNA Kit(TIANGEN). The AI-labeled ssDNA was used directly for library preparation with the TELP protocol except PCR amplification. Click chemistry was performed by adding the DBCO-S-S-PEG3-Biotin (Click Chemistry Tools, Cat. No. A112-10) to a final concentration of 400 mM, and incubated in the thermomixer (Eppendorf) for 1 h at 37°C (800 rpm). Purification steps were performed with DNA Clean & Concentrator 5 (Vistech) with the help of carrier RNA (Thermo Fisher Scientific).
GEO Accession
GSM3936208