GSM4227117: Primary neurons, D14; Mus musculus; RNA-Seq
Primary hippocampal neurons were treated with WT PFFs or PBS buffer for 7, 14, and 21 days. Total RNA was isolated using the RNeasy Mini Kit (Qiagen, Switzerland) according to the manufacturer's protocol. The concentration of each sample was measured using a NanoDrop (NanoDrop Technologies, Wilmington, DE, USA), and the purity was confirmed using the ratios at (260/280) nm and (260/230) nm. Libraries for mRNA-seq were prepared according to manufacturer's instructions with the TruSeq stranded mRNA kit (Illumina) starting from 300 ng of good-quality total RNAs (RNA quality scores >8.9 on the TapeStation 4200). Libraries were subsequently loaded at 1.44 pM on two High Output flow cells (Illumina) and sequenced in a NextSeq 500 instrument (Illumina) according to manufacturer instructions, yielding 75 nt paired-end reads.
GEO Accession
GSM4227117