GSM2674904: Rps3-TAP; Saccharomyces cerevisiae; RNA-Seq
Cells were washed, collected in ice-cold lysis buffer, flash frozen in liquid nitrogen and then pulverized in a motor and pestle. Lysate was then incubated with IgG beads for 2h and then unbound flowthrough was collected and run over a 10%-50% sucrose gradients. Samples containing bound ribosomes, eluted by TEV cleavage, and unbound ribosomes, collected from the sucrose gradients, were extracted by phenol-chloroform. For Rps3-TAP cells, only the TEV elution was used for this analysis Samples were DNase-treated, depleted of ribosomal RNA and prepared using the TruSeq Stranded Total RNA sample prep kit using standard Illumina protocols
GEO Accession
GSM2674904