Cas9 plasmid transfection of CLTA, EMX, and VEGF genomic sites from 5/13/14
Sequencing from Zuris et al. Nature Biotechnology 2014.  Three new target sites (sites CLTA, EMX, and VEGF) were chosen in the human genome that each had potential genomic off-target sites containing one identical or nearly identical half-site.  The three on-target sites and their highly similar off-target sites were amplified and sequenced from Hek293 cells treated with a mock transfection of a GFP expression plasmid or Cas9 and sgRNA expression plasmids or purified Cas9 protein and sgRNA complxed with liposomes.  All genomic target sites (for CLTA, EMX and VEGF) were combined together for each type of treatment.