Illumina HiSeq 2500 sequencing of suspended substance in rearing water: mysis 2 stage
In order to collect suspended substance, rearing water was precipitated standing for 4-6 h, and the precipitation was then filtered with sterile filter paper. Total DNA was used as the template for PCR amplication of bacterial 16S rRNA gene, using an universal primer set 515F and 806R targeting the V4 region.