GSM4278876: Ilc17_8; Caenorhabditis elegans; RNA-Seq
Worm pellets were washed twice in M9 and frozen in LN2, and 1ml Qiazol Lysis Regaent (Qiagen) and 300-400μl 0.7mm Zirconia beads (BioSpec) were added. Samples were disrupted with a TissueLyser (Qiagen), using 1min at maximum power followed by 1min on ice (repeated 4 times). RNA was extracted using the RNeasy Plus Universal Mini Kit (Qiagen), following the manufacturer's instructions with the exception that 1-Bromo-3-chloropropane (Sigma, B673) was used instead of chloroform. Libraries were prepared using the TruSeq Stranded mRNA kit (Illumina) with polyA capture for mRNA according to the manufacturer's instructions.
GEO Accession
GSM4278876