Pooled Campanulastrum americanum chloroplast sequencing
For Minnesota, Ohio, and Alabama populations field collected seed was used to germinate individuals. For Virginia field collected plants were transplanted to the greenhouse. Leaf tissue was collected from all individuals. Chloroplasts were isolated, then lysed, and DNA was obtained via a phenol-chloroform extraction and ethanol precipitation. A shotgun library was constructed with a multiplex identifier (MID) tag following standard protocols for sequencing on a Roche 454 GS-FLX platform with Titanium reagents. The MID-tagged library was sequenced as part of a larger pooled sample.