GSM3097017: A549_2; Homo sapiens; RNA-Seq
Total RNA was extracted using RNeasy mini kit (Qiagen). To obtain the final sequencing library, 14 cycles of PCR were performed using Phusion Hot Start High-Fidelity DNA Polymerase (Finnzymes, Espoo, Finland). Three biological replicates were used and for each sample replicate we obtained ~50 million paired 50-mer reads (using Illumina HiSeq 2500 platform).
GEO Accession
GSM3097017