Heliothis virescens YDK strain midgut Illumina HiSeq2000 sequences
Total RNA extracted from midguts dissected from fourth instar larvae Heliothis virescens were used to isolated mRNA using biotin labeled Oligo d(T) and magnetic beads. The first strans cDNA was synthesized from mRNA using random primers followed by RNaseH digestion and the second strand synthesis. Double-stranded cDNA from three replicated was sheared, end repaired, and dA tailed prior to ligating Illumina TruSeq indexed adapters (Indices GGCTAC, CGATGT, and TGACCA). Fifty cycles of sequencing was performed on an Illumina HiSeq2000 instrument.