RNA-Seq Belonocnema treatae Sexual Dark Pupa 1
Whole body Belonocnema treatae samples were collected from several life stages. For each sample RNA was extracted from a single individual using the Sigma-Aldrich Spectrum Plant total RNA kit and stored at -80C. Quality was assessed using an Agilent 2100 Bioanalyzer. TruSeq mRNA (Illumina) library construction and 150 bp paired-end sequencing on Illumina NextSeq500 platform were performed by University of Georgia Genomics and Bioinformatics Core. Each library was normalized by equimolar multiplexing before sequencing on a single NextSeq High output flow cell.