Whole genome resequencing and analysis of the S. eubayanus CBS12357
Genome sequencing was performed using Illumina HiSeq2000 (Illumina, San Diego, CA) at Baseclear (Leiden, The Netherlands). The genomic DNA of S. eubayanus CBS12357 strain was used to obtain a 50-cycle mate pair library with an 8 kb insert size. For a second library, fragments of ~180-200 bp were sequenced paired-end with a read length of 100 bp and afterwards the overlapping read pairs were merged into single longer (pseudo)reads. Yielding for both libraries more then 2200 Million base pair total sequence resulting in ~185x coverage.