low input RNA-seq of homo sapiens: adult peripheral CD8 T cells: Sample CD8_IGTB430_57
We sorted 7 lymphocyte populations for 6 healthy donors, in duplicate, with 1000 cells per sample. These samples were randomized in a 96 well plate and we performed Smart-seq2 library preparation, where RNA is polyA selected. Libraries were then paired-end sequenced with each read being 25bp long.