GSM3169046: MEF_undifferentiated_RNASeq_WT_rep3; Mus musculus; RNA-Seq
Total RNA was isolated using the RNeasy mini kit with on-column DNase digest (Qiagen) following the manufacturer's protocol, quantified with a Thermo NanoDrop and quality tested on Agilent Bioanalyzer. NGS libraries were prepared from 280ng RNA using the TruSeq RNA Sample Prep Kit v2 (Illumina) according to manufacturer's recommendations and amplified in 12 PCR cycles. Libraries were profiled in a High Sensitivity DNA chip on a 2100 Bioanalyzer and quantified using the Qubit dsDNA HS Assay Kit, in a Qubit 2.0 Fluorometer (Life technologies). The 12 libraries were pooled in equimolar ratio and sequenced in 7 lanes on a HiSeq 2000 Illumina sequencer, single read mode for 51 cycles plus 7 cycles for the index read.
GEO Accession
GSM3169046