SIRV Validation Experiment NGS1.61, RC0 sample, STAR pipeline
The  experiments were carried out in technical triplicates. From 1 microgram  sample RC-0 NGS libraries  were prepared by Fasteris SA  (Plan-les-Ouates, CH), using the TruSeq stranded mRNA library  preparation kit with polyA selection (Illumina). All primary library  templates were amplified with 14 PCR cycles to reach reproducible  concentrations, 155+/-45 nM, and insert sizes, 165+/-10 bp.  One  equimolar lane-mix was sequenced at the CSF Campus Science Support  Facilities GmbH (CSF, Vienna, AUT) in PE125 mode on in one lane of a  HiSeq5A flowcell using V4 chemistry. The run yielded 305 M raw reads,  293 M after demultiplexing and trimming which corresponds to a read  depth of 32.6+/-2.2 M reads per library.