GSM3937462: MG-treated + PD0325901 5h - replicate 3; Mus musculus; RNA-Seq
Micrografts (MG) were obtained from skin murine biopsies (1 cm2) using a sterile tissue destroyer (Rigeneracons®). Tissues were re-suspended in 1 mL PBS, disrupted, and filtered through 70 m filter membranes. Library preparation was performed with the Illumina TruSeq Stranded mRNA Sample Preparation Kit, according to the manufacturers protocol (48 samples, Cat No. 20020594). Denaturation of RNA was performed at 65°C in a thermocycler and cooled down to 4°C. Samples were indexed to allow for multiplexing. Sequencing libraries were quantified using the Qubit fluorometer (Thermo Fisher Scientific, Massachusetts, USA). Library quality and size range was assessed using the Bioanalyzer (Agilent Technologies) with the DNA 1000 kit (Agilent Technologies, California, USA) according to the manufacturer's instructions. Libraries were diluted to a final concentration of 2nM and sequenced on Illumina HiSeq4000 sequencing system, according to the manufacturer's recommendations.
GEO Accession
GSM3937462