GSM3618024: H2228_H1975_A549_H838_HCC827_Mixture_CelSeq3; Homo sapiens; RNA-Seq
The cells were counted using Chamber Slide and rounghly 2 million cells from each cell line were mixed and processed by 10X. Three CEL-seq2 plates were sorted for the same sample. For CEL-seq2, Single cells were flow sorted into chilled 384-well PCR plates containing 1.2ul of primer/ERCC mix using a BD FACSAria III flow cytometer. Sorted plates were sealed and immediately frozen upside down at -80C. Then these plates, together with the mRNA mixture plates, were taken from -80C and processed using an adapted Cel-Seq2 protocol with the following variations. We pooled the samples after the first strand synthesis and the second strand synthesis was performed using NEBNext Second Strand Synthesis module. NucleoMag NGS Clean-up and Size select magnetic beads were used for all DNA purification and size selection steps.
GEO Accession
GSM3618024