GSM4279204: Sample 1: HEK293 EV control cell Input; Homo sapiens; ChIP-Seq
Fixed cell pellets were sonicated in Bioruptor for 45 cycles at high setting to enrich the sonicated DNA to be ~300bp as described in our method. Then these sonicated samples samples were incubated with indicated antibodies with either protein G or A beads overnight. Then, the beads were washed 4 times with distinct buffers (see our method) and eluted with elution buffer at 65 degree for 30 min. This eluted DNA were reverse crosslinked and treated with RNAse and protease K, then the final Chipped DNA are purified with PCR cleanup kit(Qiagen). The CHIP DNA library was prepared using NEBNext Ultra II kit (NEB, #E7645L) following the instructions of the manufacture's product manual. CHIP-seq libraries were pooled together and sequenced on Nextseq 550 system using Nextseq 550 High Output Kit v2.5 or sent to UNC High-Throughput Sequencing Facility (HTSF) for sequencing.
GEO Accession
GSM4279204