Reclamation_24_Years_July_2017
The Glomeromycotan ribosomal small sub unit (SSU) was amplified from genomic DNA extracted from post-coal reclamation soils. The PCR approach was nested. The first PCR was performed using primer sets AML1 and AML2. Thereater a second PCR was performed using the template from the first PCR using primer sets AMV4.5NF and AMDGR. The partial SSU amplicon library was then processed as by following the Illumina MiSeq protocol for paired-end sequencing. Briefly, the protocol included a first stage amplicon PCR and a second index PCR using. Sequencing was done paired-end (2 X300bp) using the Illumina v3 chemistry. Following sequencing, sequences were demultiplexed and depleted of Illumina barcodes and primers by using MiSeq reporter and Trimmomatic software