NextSeq 500 paired end sequencing; Meiotic cohesin, chromatin and expression profiling of Arabidopsis thaliana floral buds from wild type and non-CG DNA methylation/H3K9me2 pathway mutant plants (ChIP-seq)
Meiotic cohesin, chromatin and expression profiling of Arabidopsis thaliana floral buds from wild type and non-CG DNA methylation/H3K9me2 pathway mutant plants (ChIP-seq)
Unopened flower buds at floral stage 9 were collected in tubes, snap frozen in liquid nitrogen and stored at -80°C until further use. Plants were grown under long day conditions (16 hours light/8 hours dark) at 20°C. Two grams of unopened floral buds were ground in liquid nitrogen and used to perform ChIP-seq for histone modifications in wild type, cmt3 or kyp/suvh4 suvh5 suvh6, as described in Choi et al. (2018) Genome Res. 28(4): 532-546. 10 ng of ChIP-purified DNA was used to generate libraries using the TruSeq Library Preparation Kit v2 (Illumina), as described in Choi et al. (2018) Genome Res. 28(4): 532-546.
Experimental Factor: genotype
wild type genotype
Experimental Factor: immunoprecipitate
anti-H3K4me2 (Abcam)