GSM4566102: C42-B_ENZ_2; Homo sapiens; RNA-Seq
Total RNA was extracted from C42-B parental and C42-B-ENZU (resistance) cells treated with enzalutamide (10uM), using RNA RNeasy kit (Qiagen). 1 μg of total RNA was subjected to library synthesis using the TruSeq V2 RNA-Seq kit. The quality of total RNA integrity (RIN) was assessed using an RNA 6000 nanochip (Agilent technologies, Lexington, MA) on a Bioanalyzer 2100 (Agilent Technologies). Libraries were prepared using the Illumina TruSeq Stranded Total RNA sample preparation kit according to the manufacturer's protocol. 50 bp single-end sequencing was performed on pooled libraries using an Illumina HiSeq 2500 platform. Sequencing reads generated were assessed for quality using FastQC. The library preps and sequencing were performed in the Genomics Core Facility at Case Western Reserve University. Differential expression was determined using Cufflinks v2.2.1. Differentially expressed genes (DEGs) were identified using a multiple testing corrected q-value < 0.05.
GEO Accession
GSM4566102