Ion Torrent Proton paired end sequencing; Lactate sensing drives fungal immune evasion
Lactate sensing drives fungal immune evasion
Cells were incubated overnight in 5 ml volumes in the indicated conditions and transferred to fresh medium in 50 mL culture flasks for 4 hours (30C, 200 rpm) to an OD600 = 0.6. RNA was obtained via Qiazol/chloroform extraction (QIAgen) according to the manufacture’s instructions and assessed for quality by nanodrop. RNA was DNase treated (TURBO DNase, Ambion) according to the manufacturer’s instructions and assessed for quality by Agilent Bioanalyser (RIN>8). Ion Total RNAseq v2
Experimental Factor: SC5314
strain
Experimental Factor: YNB Lactate
growth condition