GSM2675488: Sperm_CTRL_6; Mus musculus; Bisulfite-Seq
WGBS libraries were prepared by sonicating genomic DNA using a Covaris Sonicator, followed by end-repair, A-tailing and methylated adapter (Illumina) ligation using NEB-Next reagents. Subsequently, libraries were bisulfite treated using EZ DNA Methylation-Direct Kit (Zymo), followed by library amplification with indexed primers using KAPA HiFi Uracil HotStart DNA Polymerase (KAPA Biosystems).
GEO Accession
GSM2675488