GSM3168445: F01D4.5(baf20) rep3; Caenorhabditis elegans; RNA-Seq
1000-2000 worms per sample were collected for RNA extraction. Extraction was performed by adding 1 mL QIAzol Lysis Reagent to the worm pellet and freezing. After two freeze/thaw cycles, total RNA was extracted and purified utilizing the QIAGEN miRNeasy Mini Kit according to manufacturer's protocol. Total RNA quality was checked with an Advanced Analytical Fragment Analyzer, and RQNs greater than 7 wer accepted. Approximately 500 ng total RNA was used for library preparation by folloiwng the Illumina TruSeq Stranded mRNA Sample Preparation Guide.
GEO Accession
GSM3168445