De novo sequencing and analysis of the Panax ginseng transcriptome in the leaf-expansion period
Five year-old, field-grown ginseng (Panax ginseng C.A Meyer) the leaf-expansion period excavation from Fu song county, Ji Lin Province, China. Field-grown ginseng was cleaned, cut into small pieces and frozen in liquid nitrogen. The frozen samples were stored at -80 degrees C until use. RNA was isolated from the root of gingseng using TRIzol reagent according to the manufacturer's instructions. The RNA quality was tested using a 1% ethidium bromide-stained agarose gel electrophoresis. The 28s and 18s bands are clearly shown, with the ratio of 28s:18s band intensities greater than doubled.