RNA-Seq of mus-musculus: adult male muscle, Duchenne muscular dystrophy model
Only samples with an RNA integrity number (RIN) >7 were considered for downstream analyses.Messenger RNA (poly(A)-containing mRNA) was purified from the 1-8 g of total RNA by magnetic beads coated with oligo (dT) using a Dynabeads mRNA DIRECT Micro Kit (Thermo Fisher Scientific). To estimate the quality and quantity of mRNA samples we analyzed samples with the RNA 6000 Pico Kit with the 2100 Bioanalyzer (Agilent). The sequencing library of each RNA sample was prepared by using Ion Total RNA-Seq Kit v2 according to the manufacturers protocol (Thermo Fisher Scientific).