GSM1631190: mESC_RNAseq_shTex10-rep1; Mus musculus; RNA-Seq
RNA was extracted from ~2x10^6 cells with TRIzol (Invitrogen), separated using Phase Lock Gel tubes (5 Prime), and purified using the RNAeasy Plus kit (Qiagen) according to the manufacturer’s protocol. PolyA+ RNA was prepared for sequencing using the Illumina TruSeq RNA Sample Preparation Kit according to the manufacturer's protocol. We prepared 20 µl of 100 ng/µl total RNA for each sample to do massively parallel sequencing with the Illumina HiSeq 2500, followed by the manufactory instructions, with a poly-A selection protocol. 100-bp single end reads were generated for each sample.
GEO Accession
GSM1631190