GSM1529475: WT2 CT10; Mus musculus; RNA-Seq
Extract hippocampal total RNA  extraction (GenElute Mammalian Total RNA Miniprep Kit, Sigma). For transcriptome analysis using RNA-Seq, the quality of the isolated RNA was determined with a Qubit® (1.0) Fluorometer (Life Technologies, California, USA) and a Bioanalyzer 2100 (Agilent, Waldbronn, Germany). RNAs were then processed using The TruSeq Stranded mRNA Sample Prep Kit (Illumina, Inc, California, USA) according to the manufacturer recommendations. The TruSeq SR Cluster Kit v3-cBot-HS or TruSeq PE Cluster Kit v3-cBot-HS (Illumina, Inc, California, USA) was used for cluster generation using 8 pM of pooled normalized libraries on the cBOT. Sequencing was performed on a Illumina HiSeq 2000 system using the TruSeq SBS Kit v3-HS (Illumina, Inc, California, USA) with paired end 2 X100 reads or single end 1X100 reads.
GEO Accession
GSM1529475