Oryctes borbonicus 3kb mate pair library
Two Oryctes borbonicus beetles were dissected under sterile conditions. The thoracal flight muscles were prepared for DNA extraction with the Qiagen genomic DNA extraction kit and Qiagen genomic tip columns (Qiagen, Hamburg, Germany) following the manufacturer’s instructions. DNA quality and integrity was determined with a NanoDrop ND 1000 spectrometer (PeqLab, Erlangen, Germany) and by gel electrophoresis. The high molecular weight DNA was used to generate mate-pair libraries. Paired-end libraries were prepared with DNA extracted from beetle legs with the epicenter DNA extraction kit (Epicentre, Madison, USA) following the manufacturer’s instructions.