16s seq of BST_BAG
Paired-ends reads were assembled by aligning the forward and reverse reads using PEAR (version 0.9.5). Primers were identified and trimmed. Trimmed sequences were processed using Quantitative Insights into Microbial Ecology (QIIME 1.8) USEARCH (version 8.0.1623) and UPARSE software.Using usearch tools sequences were quality filtered, full length duplicate sequences were removed and sorted by abundance. Singletons or unique reads in the data set were discarded. Sequences were clustered followed by chimera filtered using “rdp_gold” database as reference. To obtain number of reads in each OTU, reads were mapped back to OTUs with a minimum identity of 97%. Using Qiime taxonomy was assigned using Greengenes database (Version 13_8, Aug 2013)