ldt10_week3
HBV genomic DNA was extracted using the QIAamp UltraSens Virus Kit according to the manufacturers protocol. A nested PCR was performed to amplify the HBV RT regions37, and the PCR products were purified using the QIAquick PCR Purification Kit (Qiagen, Hilden, Germany).  We used the DNA fragmentation strategy to construct shotgun fragment libraries for Ion Torrent PGM sequencing to produce shotgun reads with a target fragment size range of 200400 bp38. Library preparation was conducted using the Ion Xpress Plus Fragment Library Kit (Cat. no. 4471269, Pub. no. MAN0009847, Rev. C) with 100 ng of HBV DNA. Adapter ligation, size selection, nick repair and amplification were performed according to the manufacturers protocol.