Illumina HiSeq 4000 sequencing; 3prime RNA-seq of murine SCLC cell line modified with CRISPR-activation of Myc, Mycl or Mycn
3prime RNA-seq of murine SCLC cell line modified with CRISPR-activation of Myc, Mycl or Mycn
Cells pellets were harvested under steady state growth conditions. Total RNA was extracted using the Qiagen RNeasy Mini kit. Sequencing libraries were prepared using the QuantSeq 3' mRNA-Seq library kit (Lexogen, Austria)
Experimental Factor: genotype
lentiSAMv; Mycn sgRNA