RNA-Seq of human chordoma of the skull base
RNA extracted from FFPE skull base chordoma slides was used in the library kit Illumina TrueSeq RNA Gold, depleting ribosomal RNA with the Ribo-Zero method and enabeling strand selection. cDNA library sequencing was performed by EA-Quintiles on the Illumina HiSeq 2000 platform with 50bp paired end reads with cluster reads varying from 50 to 68 million.