GSM1303979: PDAT_11-12_C; Arabidopsis thaliana; RNA-Seq
Developing siliques of 7-8, 9-10, or 11-12 DAF were collected from a single shoot of ~50 plants of each plant line, frozen in liquid nitrogen and developing seeds removed by (Bates, P., Jewell, J., and Browse, J. (2013) Plant Methods 9, 9). Approximately 100 mg of frozen seed samples (three biological replicates for each plant line/ developmental stage) were ground to a fine power,  RNA extracted Suzuki, Y., Kawazu, T., and Koyama, H. (2004) BioTechniques 37, 542-544 , and DNA removed (DNA-Free RNA Kit, Zymo Research). Libraries were constructed using the Illumina TruSeq mRNA Library Preparation Kit v2
GEO Accession
GSM1303979