GSM2837349: KO_IL18_3; Mus musculus; RNA-Seq
Splenic NK cells were MACS enriched according to manufacturer's instructions (Miltenyi Biotec). Purity of NK cells was about 90% as determined by FACS NK cells were stained (CD45-BV650, NK1.1-PE, CD3e-APC, CD11b-BV421, CD27-FITC) and sorted on a FACSAria cell sorter (BD Bioscience). Purity of was ≥ 99%. Splenic NK cells from 6 mice per genotype were pooled in pairs and stimulated with IL-18 (MBL) (20 ng/ml for 4 h). RNA was prepared using standard Qiagen protocol. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM2837349