GSM2072093: Sample 17; Mus musculus; RNA-Seq
Individual neurons were lysed in 5µL of TCL buffer (qiagen) and total RNA was purified using RNA-SPRI beads. The Smart-seq2 protocol (Picelli et al., 2014) was used to prepare libraries. cDNA was subject to transposon-based fragmentation that used dual-indexing to barcode each fragment of each converted transcript with a combination of barcodes specific to each sample.
GEO Accession
GSM2072093