GSM2072731: TET2.FLT3_azaC_2 RRBS; Mus musculus; Bisulfite-Seq
DNA was extracted using the Allprep DNA/RNA purification Kit (Qiagen) and analyzed using a Bioanalyzer (Agilent Technologies). ERRBS libraries were prepared by the Weill Cornell Medicine Epigenomics Core according to a previously published protocol (Akalin et al., PLoSGenetics, 2012; Garrett-Bakelman, F.E. and Sheridan, C., JoVE, 2015). Briefly, high molecular weight DNA was disgested with MspI followed by end repair and adapter ligation. Size selection was perfomed for 150-400bp fragments ad bisulfite conversion was performed prior to PCR amplification.
GEO Accession
GSM2072731