GSM1627026: AG3_C2; Rhizoctonia solani AG-3 Rhs1AP; RNA-Seq
Total fungal RNA was extracted 72 hours post-inoculation using the RNeasy Plant Mini Kit (Qiagen). For treatments where the fungus was challenged with the bacteria, RNA was extracted from the zone of interaction (1cm). The peripheral fungal zone was used for RNA isolation in the controls containing only the fungus. RNA integrity was analysed after DNase I treatment (Fermentas, St. Leon-Rot, Germany) by electrophoresis on an Agilent Bioanalyzer using the RNA 6000 Nano kit (Agilent Technologies, Santa Clara. CA)  and finally 5000ng – 1ug of total RNA were sent to SciLife Lab, Uppsala and subjected to Illumina® TruSeq sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM1627026