Impact of antibiotics pretreatment on bioelectrochemical CH4 production
Total genomic DNA was extracted. Bacterial and archaeal 16S rRNA gene fragments were amplified by PCR with the bacterial specific primer set 8F/519R and the archaeal specific primer set 344F/915R. Gene fragments were then purified and ligated into the TOPO TA cloning vector. One hundred clones were selected and colony PCR was done with M13 forward and reverse primers to amplify 16S rRNA genes inserted into the vector. All plasmid inserts were sequenced at the UMass Biotechnology Center¡¯s sequencing facility, and all obtained sequences were compared to the NCBI nucleotide database with the BLASTn algorithm.