Metagenomics of GOM dead zone: site E2A
Genomic DNA was quantified using the Invitrogen qubit and sheared using the Covaris Sonicator to the desired size range. Metagenomic shotgun libraries were then generated using WaferGenŐs Apollo324 automated library system and Illumina compatible PrepX ILMN DNA library kits following the manufacturerŐs instructions. Resulting libraries were then carefully size-selected to a 180bp insert using the Sage BluePippin. Shotgun libraries were then sequenced on 2x100bp lanes of the Illumina HiSeq2000.