Col0R4
Total RNA was isolated from 100 mg of Arabidopsis leaves with the RNeasy Plant Mini Kit 50 (Qiagen), followed by an additional DNase treatment with the TURBO DNA-free Kit (Invitrogen). Preparation of RNA-seq libraries and sequencing using a 150 bp paired-end mode was performed by Novogene Biotech (Beijing, China) using standard Illumina protocols. Quality control was performed using the FastQC software.