GSM4230282: NHP_62_GVHD; Macaca mulatta; RNA-Seq
Four colon samples from the allo-HCT cohort and four colon samples from the untransplanted healthy control cohort were aseptically thawed, labeled with the viability dye LIVE/DEAD Violet (Invitrogen), then stained with αCD45 antibodies, conjugated with PerCP-Cy5.5 (BD Biosciences), and PE-conjugated αMAMU-A001 antibodies (NHP Reagent Resource). Viable LIVE/DEAD Violet-negative CD45-positive cells were sorted from two out of four GVHD-derived samples into host and donor fractions, based on MAMU-A001 expression. Sorted samples were also CD45-AF488 (IVas)-negative. Donor- and host-derived cells were defined computationally from two additional GVHD samples (see the corresponding Methods section, below). Single cell libraries from sorted samples were generated using a UMI-based droplet-partitioning platform with the Chromium single cell 3' library and gel bead kit v2 and v3 (10X Genomics) and then sequenced using a NovaSeq S2 (Illumina).
GEO Accession
GSM4230282