GSM2753982: mouse_10_2; Mus musculus; RNA-Seq
Total RNA concentration and quality from each sample was assessed using Qubit 2.0 fluorometer (ThermoFisher Scientific) and Agilent TapeStation 2200 (Agilent, Santa Clara, CA) Total RNA libraries were generated using Illumina TruSeq Stranded Total RNA Sample Preparation Guide Rev. E (Illumina, San Diego, CA). In brief, this process depletes nuclear ribosomal RNA (rRNA) and then fragments the remaining RNA, which was converted into first strand cDNA using reverse transcriptase and random primers. The second strand cDNA is generated using DNA polymerase I and RNase H. The cDNA fragments were further processed for adapter ligation, and then enriched with PCR to create the final cDNA library.
GEO Accession
GSM2753982