GSM3168904: 223 2018: RIP2 KO3 Input; Mus musculus; RIP-Seq
Mammary glands were incubated with collagenase/hyaluronidase solution (STEMCELL Technologies), then red blood cells were lysed and cells were further dissociated with trypsin (Sigma), Dispase II (Sigma) and DNAse I (Sigma). Then, epithelial enriched fraction was obtained by EasySep (STEMCELL Technologies). Poly A purification ( New England Biolabs, catalog # E7490) was only performance on Input samples in only one step of purification. Library generation was done with the kit NEBNext Ultra II RNA Library Prep Kit (New England Biolabs, catalog # E7770), following manufacturer´s instructions and 10-13 cycles of amplification.
GEO Accession
GSM3168904