GSM4279137: ChIP_p50_BMDM_KLA; Mus musculus; ChIP-Seq
Bone marrow was isolated from C57BL/6J mice and differentiated for 7 days using media containing M-CSF to bone marrow-derived macrophages (BMDMs) as described previously (Link et al., 2018). BMDMs were then treated with 10 ng/mL KLA (Avanti Polar Lipids) for 1 hour. ChIP-Seq sequencing libraries were prepared from recovered DNA by blunting, A-tailing, and adapter ligation using barcoded adapters (NextFlex, Bioo Scientific) as previously described (Heinz et al., 2010; Gosselin et al., 2014). Libraries were PCR-amplified for 12-15 cycles and size selected for fragments (225-400 bp) by gel extraction (10% TBE gels, Life Technologies EC62752BOX). Libraries were single-end sequenced for 51 cycles with an average sequence depth >20 million on an Illumina HiSeq 4000 platform (Illumina, San Diego, CA) according to manufacturer's instructions.
GEO Accession
GSM4279137