GSM2415913: Input.Hippocampus; Mus musculus; ChIP-Seq
For the in vivo ChIP, hippocampal tissue pooled from two animals was finely minced and cross-linked with formaldehyde (1% final concentration) for 15 minutes at room temperature, followed by glycine quenching for an additional 10 minutes at 4°C. To create a single cell suspension, samples were washed once with ice cold PBS and homogenized by passing through a 22G needle 10 times. Immunoprecipitation was performed using protein A Dynabeads (Life Technologies). Sequencing libraries were prepared using NEBNext Ultra library preparation procedure, and then assessed for quality and quantity by BioAnalyzer (Agilent) and qPCR (Kapa Biosystems). Sequencing was performed on the Illumina NextSeq 500 platform.
GEO Accession
GSM2415913