HeLa-attached Escherichia coli O157 transcriptomes
HeLa-attached O157, logarithmic E. coli O157:H7 EDL933 was co-cultured with HeLa cells for 3 h, non-adherent bacteria were removed through extensive washing with pre-warmed PBS 6 times. The eukaryotic cells were lysed using lysis buffer (95% ethanol, 5% phenol, and 0.1% SDS), and the adherent bacteria were collected as the experimental samples.