WXS of MCF7RN-TamR cell line
"Cells were maintained in RPMI/1640 medium, supplemented with 10% heat inactivated fetal bovine serum (FBS) and 1% penicillin/streptomycin/glutamine (PSG). The TamR cells were kept in phenol-red free medium supplemented with 10% heat-inactivated charcoal-stripped-FBS and 1% PSG, with the addition of 100 nM 4HT. Genomic DNA was isolated using a Wizard genomic DNA purification kit (Promega), using manufacturer's protocol. Exome libraries were generated using the Agilent SureSelect XT kit and Agilent Automation Systems NGS system per manufacturer's instructions."