GSM3097765: FSHD2B_MB; Homo sapiens; ncRNA-Seq
Total RNA from myoblasts (MB) and myotubes (MT) from two control and two FSHD2 individuals was isolated using TRIZOL (Invitrogen) according to the manufacturer's instructions. The isolated RNA was treated with DNase twice to remove contaminating DNA prior cDNA generation.  First, isolated RNA was treated with RNase-free DNaseI (Sigma-Aldrich, #04716728001 ROCHE) followed by acidic phenol/chloroform extraction, and then treated with TURBO RNase-free DNase (Ambion) followed by inactivation reagent treatment. The DNaseI-treated RNA was tested for genomic DNA contamination by PCR and RNA quality by RNA gel. small RNA libraries were prepared using Illumina TruSeq small RNA protocol
GEO Accession
GSM3097765