GSM3557087: 103133_NCAR_3_IN; Mus musculus; RNA-Seq
Input and IP samples underwent DNA depletion and RNA purification with the RNeasy Micro Plus kit (Qiagen). Integrity of the eluted RNA was analyzed by a 2100 Bioanalyzer (Agilent). Sequencing libraries were prepared using Illumina TruSeq RNA sample prep kit following manufacturer's protocol.  After library preparation, amplified double-stranded cDNA was fragmented into 125 bp (Covaris-S2, Woburn, MA) DNA fragments, which were (200 ng) end-repaired to generate blunt ends with 5'- phosphates and 3'- hydroxyls and adapters ligated.
GEO Accession
GSM3557087