WGS of Saccharomyces cerevisiae BY0012_subclone2
Genomic DNA was isolated from the lineages after 24 h growth of the cultures following inoculation in the form of a streak on YPD agar from stocks stored at 70 C. DNA isolation followed Hanna and Xiao (2006). Library preparation was performed using tagmentation with the Nextera DNA Flex Library Prep kit (Illumina, San Diego, CA, USA) according to the manufacturers protocol, sequencing was performed using 150 bp paired-end reads on an Illumina NextSeq 500 system, with approximately 50 coverage of the nuclear genome.