qiime_ppdid_136:10058.000007850
stool samples from American gut Project
This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.
barcodesequence
GCATATGCACTG
center_name
CCME
center_project_name
American gut 15
emp_status
EMP
experiment_center
CCME
experiment_design_description
stool samples from American gut Project
experiment_title
American Gut 15
extraction_robot
unknown
extractionkit_lot
unknown
file_path
/projects/qiita_data/working_dir/vpPo9i/10058.000007850.fastq.gz
file_type
fastq
illumina_technology
MiSeq
library_construction_protocol
This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.
linker
GT
linkerprimersequence
GTGCCAGCMGCCGCGGTAA
mastermix_lot
unknown
orig_name
7850
pcr_primers
FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
platform
Illumina
plating
unknown
primer_date
unknown
primer_plate
2
processing_robot
unknown
project_name
AGP15
run_center
CCME
run_date
8/21/14
run_prefix
Undetermined_S0_L001
samp_size
.1,g
sample_center
CCME
sample_plate
AG_41
sequencing_meth
sequencing by synthesis
study_center
CCME
target_gene
16S
target_subfragment
V4
tm1000_8_tool
unknown
tm300_8_tool
unknown
tm50_8_tool
unknown
water_lot
unknown
well_id
C9