GSM4001115: WT_rep1; Mus musculus; OTHER
Bone marrow was extracted from mice legs, B220+CD43+IgM- pro-B cells were isolated on a FACS Aria sorter (BD Biosciences) and genomic DNA was exctracted by using Quick-DNA Miniprep Plus Kit from ZYMO (Cat#D4068). 1ug of genomic DNA/sample was sonicated to 350bp fragments. Antibody immunoprecipitation: Resulting DNA was incubated and isolated with anti- 5hmC antibody and magnetic beads Libraries were constructed according to Illumina standard protocols and were preceded by end repair reaction and adaptor ligation. Amplification of the purified DNA with specific primers