ITS METAGENOMIC SEQUENCING OF TOMATO PHYLLOPLANE
After obtaining the Qubit concentration for the library and the mean peak size from Bioanalyser profile, library was loaded onto Illumina platform at appropriate concentration (10-20pM) for cluster generation and sequencing. Paired-End sequencing allows the template fragments to be sequenced in both the forward and reverse directions on Illumina platform. The kit reagents were used in binding of samples to complementary adapter oligos on paired-end flow cell. The adapters were designed to allow selective cleavage of the forward strands after re-synthesis of the reverse strand during sequencing. The copied reverse strand was then used to sequence from the opposite end of the fragment.