GSM1303999: H3-K36Me3 (Replicate 2); Schizosaccharomyces pombe; ChIP-Seq
Essentially as in: Keogh et al (2004) Methods Mol Biol 257:1-16. Input or ChIP samples were converted to barcoded libraries by adaptor addition as previously (Quail et al (2008) Nat Methods 5:1005). In brief each sample was quantified, end-repaired (End-It kit, Epicenter Biotechnologies), an A-overhang added, and ligated to one of a set of 12 barcode-specific adaptor primers. The resulting libraries were size selected (600 ± 50 bp), amplified by 18 cycles of PCR, purified with SPRI beads (Agencourt AMPure XP; Fisher Scientific), quantified on a high-sensitivity DNA chip by 2100 bio-analyzer (Agilent Technologies, Inc.), mixed at equivalent concentrations, and multiplexed at 12 samples per Illumina HiSeq 2000 lane.
GEO Accession
GSM1303999