GSM2334686: CMK9_Naive-like_MS091_038; Macaca fascicularis; RNA-Seq
Total RNA was extracted from Cm somatic cells, prime state Cm PSCs, Naive-like state Cm PSCs, and differentiated Cm PSCs by using an RNeasy Mini Kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions. Synthesis and amplification of cDNAs using 1 ng of purified total RNA, and construction of cDNA libraries for RNA sequencing (ABI SOLiD 5500XL system; Life Technologies) were performed as described in (Nakamura et al., 2015). cDNA synthesis and amplification from isolated RNAs/single cells were perfomed essentially as reported in [Kurimoto, K.et.al., Nucleic Acids Research, 34(5), e42.]. Library constructions for Nextseq500 were performed as reported in [Ishikura et al. submitted].
GEO Accession
GSM2334686