RNA-Seq of selected sequences at round 0
Library preparation was performed by 2-step PCR approach. Briefly, PCR1 was performed with aptamer specific primers with partial adapters. A second PCRwas performed to complete the full sequencing adapter. Library quality was assessed by gel electrophoresis, Agilent bioanalyzer and qPCR. Sequencing wasperformed on the Illumina platform using 2 x 75bp paired end sequencing configuration.