GSM4227451: RDS_SO-03-46_Lu; Rattus norvegicus; RNA-Seq
Total RNA, including miRNA and other small RNA molecules, was isolated from a piece of the lung tissue using the miRNeasy Mini Kit (Qiagen, Inc.; Valencia, CA) following the procedure provided by the manufacturer, including optional DNase treatment with RNase-Free DNase (Qiagen, Inc.; Valencia, CA). One microgram total RNA/sample was used to create sequencing libraries using the Illumina TruSeq® Stranded Total RNA Library Prep with Ribo-Zero Gold  (Illumina, Inc., San Diego, CA) following the  manufacturer's protocol with the exception of only 12 PCR cycles used to enrich the DNA fragments.
GEO Accession
GSM4227451