WXS-Seq of canis lupus familiaris: adult male PDX
Sequencing libraries were prepared using the Roche Kapa HyperPrep Library prep Kit. 178 During adapter ligation, unique indexes were added to each sample. Resulting libraries were 179 cleaned using SPRI beads and quantified on the Qubit 2.0. Size distributions were checked on an 180 Agilent Bioanalyzer. Libraries were pooled into equimolar concentration (8 libraries per pool) 181 and library pools were finally enriched using the Roche SeqCap EZ Dog Exome panel (design 182 1000003560). Each pool of enriched libraries was sequenced on one lane of a HiSeq 4000 flow 183 cell at 150bp PE, generating about 41 Million clusters per sample or ~12Gb of data. Sequence 184 data was demultiplexed and Fastq files generated using Bcl2Fastq2 conversion software 185 provided by Illumina.