GSM2795870: H2BV replicate 1; Toxoplasma gondii RH; ChIP-Seq
Parasites were lysed in lysis buffer to release nuclei. Nuclei were digested with micrococcal nuclease and centrifuged to remove cell debris and harvest digested DNA. Histone-DNA complexes were immunoprecipitated with protein A-conjugated magnetic beads and digested with proteinase K before DNA purification. Adapters were ligated to DNA using a TruSeq ChIP-seq kit (Illumina). Libraries were sequenced using a Hiseq2500 sequencing machine (Illumina). This involves a first step of cluster generation in situ on the flow cell followed by massively-parallel sequencing using fluorescently-labeled reversible terminator nucleotides. Clustered templates were sequenced base-by- base during each read. Images generated by the Hiseq2500 were processed by Hiseq Sequence Control Software (HCS V2 or latest with RTA v1.17 or latest and CASAVA 1.8 or latest software packages). These software components perform the functions of image analysis (Firecrest) and base-calling (Bustard).
GEO Accession
GSM2795870