1994D
Total RNA was extracted from a single soybean embryonic axis. mRNA was enriched by hybridization to oligo(dT) beads and cDNA was synthesized with a strand-switching protocol using a poly(T) anchored primer and a strand-switching primer. cDNA was amplified and a unique barcode was added. Five libraries were pooled before sequencing on a MinION R9.4 flow cell.