GSM2413631: IP2_RIP_mouse; Mus musculus; RIP-Seq
Flag-Samd4 expressing Samd4PB/PB osteoblasts were resuspended in IP buffer (50 mM Tris-HCl Ph 7.5, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate and 1mM phenylmethanesulfonyl fluoride (PMSF)), subjected to gentle sonication, and centrifuged to obtain cell extracts. Libraries were prepared according to Illumina's instructions accompanying the RNA Sample Kit. The purified RNA was captured on an Illumina flow cell for cluster generation.
GEO Accession
GSM2413631