GSM3618080: N10-220317; Mus musculus; RNA-Seq
Total RNA was isolated with the miRNeasy micro kit (Qiagen) Double stranded cDNA for RNA-seq library preparation was generated using SMART-Seq v4 Ultra Low Input RNA reagents (Catalog Number 634888, Clontech, Mountain View, California, USA) according to the protocol provided with the reagents beginning with 4ng of total RNA and using 9 cycles of PCR. 300 pg of the resulting cDNA were used for library preparation with the Illumina Nextera XT DNA Library reagents (Catalog Number 15032354, Illumina, San Diego, California, USA) using the single cell RNA-seq library preparation protocol developed for the Fluidigm C1 (Fluidigm, South San Francisco, California, USA).
GEO Accession
GSM3618080