TCR-seq of Homo sapiens:GO
RNA was isolated using Trizol reagent (Invitrogen, USA) according to manufacturer protocol. During the first round of PCR, amplification of TCR CDR20 using the HTBI primers (Huntsville, Alabama, America) was carried out by Qiagen OneStep RT-PCR. The firstround PCR product was used as template for a second step of amplification to construct the libraries, and multiplex PCR Kit (Hilden, Nordrhein-Westfalen, Germany) were use