GSM2677608: Total-Pol-II_ChIPseq_NOAA; Homo sapiens; ChIP-Seq
Cells were crosslinked with 1% formaldehyde. Cell lysates were cleared from sonicated nuclei and protein-DNA complexes were isolated with indicated antibodies. DNA obtained from ChIP experiments was quantified using Qubit assays (Invitrogen #Q32854). 2 ng DNA from each ChIP and corresponding input samples were used to prepare libraries using the NuGEN Ovation Ultralow Library System (NuGEN Technologies #0331) or the KAPA LTP kit (KAPA Biosystems #8230) and sequenced with Illumina Hi-seq platforms to obtain 50 bp-long reads.
GEO Accession
GSM2677608