HEG4 individual 1, 3 kb library
Genomic DNA was extracted using the CTAB method (HUANG et al. 2000). DNA quantity was checked with a NanoDrop spectrophotometer (NanoDrop products, Wilmington, DE, USA). Sequencing libraries were constructed using the Illumina TruSeq DNA Kit version C (Illumina Inc., San Diego, CA, USA) and quantified by Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). Libraries were multiplexed to 10nM and were sequenced on a HiSeq2000 pair-end 100bp run in the UCR IIGB Genomics Core Facility (http://illumina.ucr.edu). The Illumina CASAVA 1.7.0 pipeline was used for base calling and de-multiplexing.