P1_d0a_large_DNA
Microbes from  incubations were then filtered through a 0.2 m filter and these filters were  inserted into the Qiagen DNease Blood and Tissue DNA Isolation Kit. The  extracted bacterial DNA underwent amplicon library preparation and Illumina  sequencing of the 16S-V4 rRNA hypervariable region using 16S-V4 515f and 806r  primers on a MiSeq system.