GSM3168492: H3K27ac ChIP-seq EB AC; Mus musculus; ChIP-Seq
Cells were cross-linked with 1% formaldehyde at 37 °C for 10 min and quenched with 0.125 M glycine for 5 min at room temperature. Then cells were scraped and ChIP was performed and DNA were extracted using a ChIP assay kit (Millipore) as described. For library construction and sequencing, ChIP-Seq DNA samples were quantified and quality assessed by Qubit 2.0 and Agilent 2100. DNA fragments range from 200-600bp were selected constructed for ChIP-Seq library with TruSeq ChIP Sample Prep Kit (Illumina) according to manufacturer's instructions. After scraped from plate, embryonic stem cells were washed with PBS and incubated in lysis buffer.
GEO Accession
GSM3168492