GSM3168463: D17-188001: Basal 37C, Rep1; Homo sapiens; RNA-Seq
Cells were trypsinized and RNA was extracted using the Qiagen RNeasy Plus Mini Kit and QIAshredder homogenization columns. For RNAseq, RNA integrity and concentration were checked on a Fragment Analyzer (Advanced Analytical) and libraries were prepared using the Kapa HyperPrep RNAseq kit (Roche). Libraries were quantified using the Fragment Analyzer (Advanced Analytical) and qPCR before being loaded for single-end sequencing using the Illumina HiSeq 2500 for 40 nucleotides. polyA-based mRNA capture using Kapa Hyperprep strand-specific strategy.
GEO Accession
GSM3168463