GSM4678177: Naïve - Rep1 (IP); Mus musculus; RIP-Seq
Mice were euthanized 1 hour post-restraint, brains were removed, and the lateral septi were dissected on ice and pooled into 3 replicates per group of 4-6 mice each. Tissue was homogenized and clarified by centrifugation. Ribosomes were immunoprecipitated by using 4 ug of polyclonal antibodies against pS6 (Invitrogen) previously conjugated to Protein A-coated magnetic beads (Thermofisher). A small amount of tissue RNA was saved before the immunoprecipitation (Input) and both input and immunoprecipitated RNA (IP) were then purified using RNAeasy Mini kit (QIAGEN). RNA quality was checked using a RNA PicoChip on a bioanalyzer. RIN values > 8 were used. cDNA was amplified using SMARTer Ultralow Input RNA for Illumina Sequencing Kit and sequenced on an Illumina HiSeq2500 platform.
GEO Accession
GSM4678177