GSM2150334: KasumiSCR_ChIPSeq; Homo sapiens; ChIP-Seq
Chromatin was fixed in 1% formaldehyde for 10 minutes, follwed by shearing them to 200-700bp using a Bioruptor (Diagenode). Samples were immunoprecipitated using AML1-ETO (Diagenode, Cat. C15310197; Lot A706-001) antibody. Sequencing samples were prepared according to the manufacturer's protocol (Illumina). End repair was performed using the precipitated DNA using Klenow and T4 PNK. A 3’ protruding A base was generated using Taq polymerase and adapters were ligated. The DNA was loaded on gel and a band corresponding to ~300 bp (ChIP fragment + adapters) was excised. The DNA was isolated, amplified by PCR and used for cluster generation. ChIP-seq libraries were further processed according to Illumina's standard protocol.
GEO Accession
GSM2150334