ESRP overexpression (MDA-MB-231 celline)
10ug total RNA was used to prepare polyA RNA for fragmentation followed by cDNA synthesis with random hexamers and ligation to Ilumina adaptor sequences. The samples were quantified using an Agilent 2100 Bioanalyzer, loaded onto flow-cells for cluster generation, and sequenced on an Illumina GenomeAnalyzer IIx using single-read protocol to generate 76bp reads (Illumina).