Single-molecule real-time sequencing of Fragaria iinumae
An SMRTbell DNA library was prepared and sequenced according to the manufacturers protocols (Pacific Biosciences, CA, USA), and a 20-kb SMRTbell library was generated using a BluePippin DNA size selection instrument (Sage Science, MA, USA) with a lower size limit of 10 kb. Single-molecule real-time sequencing of long reads was conducted on a PacBio Sequel platform with 9 SMRT cells.