RNA-seq of S.cerevisiae: _msh2 ancestral in Glycerol stress (YPG) replica 1
Total RNA was depleted of ribosomal RNA using Ribo-Zero Gold rRNA removal yeast kit (Illumina, WI). Indexed libraries RNAseq libraries were constructed using strand-specific cDNA synthesis (TruSeq stranded mRNA preparation kit, Illumina) from oligo-dT captured mRNAs, pooled in equimolar concentration and subjected to single-end 75bp Illumina sequencing in an Illumina NextSeq500 platform using High Output 75 cycles kit v2.0 (illumina). All performed at the Genomic core facility at SCSIE from University of Valencia (Valencia, Spain)