VSV_ko_RNA-seq_rep1
Total RNA was extracted with TRIzol reagent (Invitrogen, 15596-018) from RAW264.7 cell treated with VSV or PBS control for 12 hours, mRNA was purified from total RNAs using Dynabeads mRNA purification kit (Ambion, 61006). RNA-seq libraries were directly generated using the KAPA Stranded Mrna-Seq Kit (KAPA, KK8401) following the manufacturers instructions, and then sequenced using the Illumina HiSeq X Ten platform.