GSM1411620: Input; Homo sapiens; ChIP-Seq
After cell lysis, DNA was sheared to fragments of ~200bp and lysate was cleared by centrifugation. 25% of the supernatant was used as input and the remaiining part was used to precipitate coilin with Dynabeads Protein G coupled to goat anti-EGFP antibody. After stringent washing, the DNA was eluted and de-crosslinked by Proteinase K treatment. DNA fragments were purified by phenol-chlorophorm extraction. Chipped DNA was end repaired with End Repair Module and A-Tailed with dA-Tailing Module (NEB). Universal Illumina adaptors were ligated to DNA ends and amplified using PCR Enrich Adaptor Ligated cDNA Library module (NEB). Products were purified and size selected on 2% Agarose gel.
GEO Accession
GSM1411620