Illumina HiSeq 2500 paired end sequencing; Pericyte induced reprogramming of the bone marrow microenvironment drives stem and cancer cell quiescence
Pericyte induced reprogramming of the bone marrow microenvironment drives stem and cancer cell quiescence
This dataset was generated by RNA sequencing of whole tibiae of C57Bl/6 mice. For young mice; tibiae from 6-weeks-old mice were used and for aged mice tibiae from 70-weeks-old mice were used. The control, radiation, and chemotherapy samples were generated using 14-weeks-old mice. For radiation treatment; mice were exposed to sublethal irradiation while for chemotherapy samples mice were treated with carboplatin and tibiae were collected. Adult C57BL/6J wild-type mice were whole-body irradiated with a single dose of 900 rads (Gammacell irradiator) and euthanized 5 days later. For chemotherapy, adult C57BL/6J mice were treated with carboplatin or cisplatin as indicated. Both carboplatin and cisplatin were prepared fresh in saline. Carboplatin was injected in traperitoneally 30 mg/kg/mouse thrice each week for six doses. Qiagen RNeasy Plus Micro Kit Illumina TruSeq RNA Library Preparation Kit v2 with RiboZero
Experimental Factor: age
6
Experimental Factor: compound
none
Experimental Factor: irradiate
none