NE2C1 RNAseq
RNA extraction was performed using the InviTrap Spin Plant RNA Mini Kit (Stratek Molecular) following the manufacture's protocol. We used the provided RP buffer for lysis. RNA concentrations and purity were tested by Nanodropmeasurement (ND-1000, peQLab). RNA integrity was checked with an automated electrophoresis station Experion (Bio-RAD), using StdRNA chips and reagents (Bio-RAD). RQI values were >7.2.  Library preparation proceeded with DNase 1 digestion of total RNA, mRNA isolation by use of oligo(dT) beads, mRNA fragmentation, first and second strand cDNA synthesis, end-repair, A-tailing, bar-coded adapter ligation and PCR amplification. Sequencing libraries were checked using an Agilent 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany) before sequencing.  Single-end (1 x 100 bp) RNA Sequencing (RNASeq) data were generated using standard Illumina protocols and kits (TruSeq SBS KIT-HS v3, FC-401-3001; TruSeq SR Cluster Kit v3-cBot-HS, GD-401-3001) and all sequencing was performed using the HiSeq 2000 platforms (University of Groningen Genome Analysis Facility).