GSM1527074: vp64_myod.rep1; Homo sapiens; RNA-Seq
The total cellular RNA from a confluent 15cm plate of human dermal fibroblasts was isolated using the RNeasy Plus RNA isolation kit (Qiagen). PolyA+ RNA was subsequently purified using oligo-dT beads (Invitrogen). First strand cDNA was generated using SuperScript Vilo cDNA synthesis master mix (Invitrogen), and second strand cDNA was synthesized using E. coli DNA Polymerase I (New England Biolabs) with random hexamer primers. Double stranded cDNA was purified using 1.8X Agentcourt SPRI beads (Beckman Coulter). Double stranded cDNA was fragmented and sequencing adaptors added using Nextera transposase (Illumina) and indexed for sequencing by PCR as described previously. Samples were pooled in equimolar ratios and sequenced on an Illumina HiSeq 2000.
GEO Accession
GSM1527074