RNA-seq of P. lunatus domesticated accession G27455. Pod tissue. Start pod elongation. Biological replicate 2
RNA samples were quantified with Nanodrop 2000 and quality was assessed with Agilent Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA) and agarose gel electrophoresis. Samples showing a 260/280 ratio of absorbance between 1.9 - 2.0 and RIN (RNA Integrity Number) values above seven were selected. mRNA samples were enriched using oligo(dT) beads and randomly fragmented, then cDNA was synthesized using mRNA templates. RNA-seq library preparation was completed through size selection and PCR enrichment. Sequencing of the library was accomplished using a paired-end 150 run (2150 bases) on an Illumina HiSeq Platform (Illumina, San Diego, CA , USA).