GSM2335051: Replicate1,chrRNA-seq WildType; Homo sapiens; OTHER
Lysates were clarified from sonicated nuclei and RNA:DNA hybrids were isolated with antibody for RNA-DIP-seq For the RNA DIP-seq, the RNA component of the R-loop was used to prepare the library using NEBNext Ultra Directional RNA Library Prep Kit for Illumina, v1.0 (E7420) according to manufacturer's guidelines. For the ChhrRNA-seq, chromatin associated RNA was used for library prepration as for RNA DIP-seq
GEO Accession
GSM2335051