GSM4228180: hIslets_III_DMSO1_36hr; Homo sapiens; RNA-Seq
Islet dissociation was performed by incubating the islets in accutase for 20 min at room temperature. Reaction was stopped by adding CMRL media with 10% FBS. The dissociated islets were filtered through a 40 µm filter to obtain a single-cell suspension. Single-cell RNA-seq was performed using the Single Cell 3' Library & Gel Bead Kit v2 (10x Genomics) according to the manufacturer's protocol.
GEO Accession
GSM4228180