RNA-Seq_ZB_larvae
RNA was prepared for library builds using the TruSeq RNA Sample Preparation Kit v2 (lllumina, San Diego, CA). When the library build was completed, sample quality and average fragment size were assessed with the Advanced Analytics Fragment Analyzer using the High Sensitivity NGS Fragment Kit (Agilent Technologies). Quantity was assessed with a Kapa Library Quantification kit for Illumina NGS (Kapa Biosystems, Boston, MA). After final library QC was completed, equimolar sample was pooled and clustered for sequencing on the HiSeq2500 system (Illumina). The sequencing run was performed using Illumina Rapid-Run SBS chemistry (TruSeq Rapid SBS Kit, Illumina) in paired-end mode with a read length of 100 bp paired-end reads (2x100PE) bp.