GSM3935841: LK2DG3: LKR10_2D_shGFP_Rep3; Mus musculus; RNA-Seq
TRIzol extraction according to manufacturers protocol, followed by cleanup and Dnase treatment using the RNeasy kit columns with DNase treatment, also according to manufacturers protocol. RNA was enriched using oligo(dT) beads. The mRNA was then fragmented randomly by adding fragmentation buffer, then the cDNA synthesized by using the mRNA template and random hexamers as primers, after which a custom second-strand synthesis buffer (Illumina), dNTPs, RNase H and DNA polymerase I are added to initiate the second-strand synthesis. After a series of terminal repair, ligation and sequencing adaptor ligation, the double-stranded cDNA library was size selected and PCR enriched.
GEO Accession
GSM3935841