GSM2677313: PASS4 in L-Asp, repl 1; Pseudomonas aeruginosa; RNA-Seq
RNA was extracted from these cultures using the miRNEasy RNA extraction kit (Qiagen) according to the manufacturer’s protocol. To remove any residual genomic DNA, the samples were treated with DNase using the TurboDNase kit (Invitrogen). To remo.ve highly abundant ribosomal RNA from the RNA extracts before sequencing, the samples were treated using RiboZero GN Magnetic rRNA depletion kit (Epicentre); the rRNA depleted samples were purified using RNeasy MinELute Cleanup kit (Qiagen) RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM2677313