GSM4626855: Decato-2018-Mouse_4T1K; Mus musculus musculus; Bisulfite-Seq
The parental mouse mammary tumor cell line 4T1 (ATCC) and the derived clonal cell lines were cultured as described in \cite{wagenblast2015model}. Genomic DNA was extracted using the QIAmp DNA Blood Mini Kit (Qiagen). Bisulfite sequencing libraries were generated as previously described \cite{hodges2011primaryBloodMeth} In short, genomic DNA was fragmented using the Covaris LE220 sonicator to a target fragment size of 200bp. DNA fragments were repaired and ends blunted and phosphorylated. Then, adenylated fragments were ligated to Illumina-compatible paired-end adapters. Subsequently, DNA fragments were purified using the MinElute PCR Purification Kit (Qiagen). Fragments were then denatured and treated with sodium bisulfite using the EZ DNA Methylation-Gold Kit (Zymo Research). Then, the sample was desulfonated and PCR amplified with High Fidelity Expand Plus (Roche) using paired-end adapter-compatible primers. Illumnia sequencing was performed, generating 76-nucleotide paired-end reads.
GEO Accession
GSM4626855