GSM4189160: CC035_lung_atac; Mus musculus; ATAC-seq
Mice were euthanized by exsanguination following deep nembutal (100 mg/kg intraperitoneal injection) anesthesia. Lungs, livers, and kidneys were excised, snap-frozen in liquid nitrogen, and stored at –80°C. Flash frozen tissue samples were pulverized in liquid nitrogen using the BioPulverizer (Biospec) to break open cells and allow even exposure of intact chromatin to Tn5 transposase. Pulverized material was thawed in glycerol containing nuclear isolation buffer to stabilize nuclear structure and then filtered through Miracloth (Calbiochem) to remove large tissue debris. Nuclei were washed and directly used for treatment with Tn5 transposase TruSeq libraries were constructed as per manufacturer's recommendations
GEO Accession
GSM4189160