Miseq of Rhizobium leguminosarum sv. trifolii: rpoB, recA, nodA and nodD amplicons from Store Heddinge T. repens nodules.
DNA was isolated from T. repens nodules collected from Store Heddinge, Denmark. Individual PCRs using Phusion (proofreading) polymerase for rpoB, recA, nodA and nodD Rlt genes were pooled for each sample.