GSM2416605: IGF_34b1; Homo sapiens; RNA-Seq
Small-needle paired renal biopsies immediately after collection were placed in RNA®later (Ambion, USA), processed and stored for further analysis.  Total RNA was isolated using TRI® reagent, on column DNase treated, further purified (Zymo Research)  and  stored at -80°C.  RNA samples with RIN>6 were used for downstream analyses. 400 ng of total RNA and TruSeq Stranded Total RNA Ribo-Zero H/M/R Gold kit (Illumina) was used to create libraries for downstream NGS. QA and QC was performed for each library.
GEO Accession
GSM2416605