Degradome of sugarcane control:leaf
Total RNA from sugarcane leaves was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturers procedure. Approximately 150 ng of poly(A)+ RNA was used as input RNA and annealing with Biotinylated Random Primers. Strapavidin capture of RNA fragments through Biotinylated Random Primers. 5 adaptor ligation to to only those RNAs containing 5-monophosphates. Reverse transcription and PCR . Librariy was sequenced using the 5adapter only, resulting in the sequencing of the first 36 nucleotides of the inserts that represented the 5ends of the original RNAs. And then we performed the single-end sequencing (50 bp) on an Illumina Hiseq2500.