GSM2676189: 1010.MT; Homo sapiens; RNA-Seq
Tissue (~10 mg) was immersed in 2 mL of hypotonic buffer A [10 mM HEPES (pH 7.9), 2 mM MgCl2, and 2 mM KCl] supplemented with protease and phosphatase inhibitors (Thermo, 78441) and 40 U/mL ribonuclease inhibitor (Invitrogen, 10777-019). RNA was extracted with standard Ion Torrent protocol. The RNA concentration was measured using a Nanodrop instrument. RNA-sequencing library was prepared from 200 ng of total RNA using Ion Total RNA-seq Kit v2 (standard Ion Torrent protocol)
GEO Accession
GSM2676189