transcriptome profiling of bowhead whale (Balaena mysticetus) liver 1
Total RNA was isolated from frozen tissues using a RNAqueous kit (Life Technologies, Carlsland, CA, USA). Short-insert ‘paired-end’ libraries were prepared using the Illumina TruSeq Sample Preparation Kit v2 and Illumina HiSeq 2000 next-generation sequencing was performed following the manufacturer’s instructions (Illumina, San Diego, CA, USA). Libraries were sequenced bi-directionally (101 bp in each direction) on the Illumina HiSeq 2000 Genome Analyzer platform.