DWTP-6-2
Filter material (15 mL each) was collected from the top of each filter using a 1% hypochlorite-wiped stainless steel grab sampler. Filter material was immediately placed into cryotubes, then immersed in liquid nitrogen, and stored at -80 ¡C for further analysis. Extraction of DNA from 0.5 g of sand material from each sample was carried out using the MP FastDNA Spin Kit. DNA was amplified using amoA Nitrospira primers Ntsp-amoA 162F and Ntsp-amoA 359R. Library preparation and Illumina MiSeq sequencing was carried out by DMAC (DTU Multi-Assay Core Facility, Kgs Lyngby, DK).