Sample 2 from anaerobic digestion of pig manure
Sample "Biograss_Pig_manure_PM2" was collected from steady state reactors digesting swine manure. Plant material was removed from samples of pig manure by filtering with a 40-μm nylon cell strainer filter and by centrifugation (500 x g for 15 min). Supernatant was centrifuged for 20 min, 10000 g at 4°C and resultant cell pellet was processed to extract genomic DNA  The genomic DNA was processed according to PowerSoil® DNA Isolation Kit protocol. DNA libraries were prepared with Nextera XT kit (Illumina, San Diego, CA) and samples were sequenced using NextSeq 500 sequencing technology producing 150 bp paired end reads.