GSM4280155: B4_plate 2B_E10.5_DP; Mus musculus; RNA-Seq
GFP+ ventral foregut (E8.5), pancreatic (E10.5, E14.5), and hepatic progenitors (E10.5) were dissected from Tg(Prox1-EGFP) transgenic mice and dissociated into single-cell suspensions. GFP+ cells were FACS sorted at 4°C using an Aria II or Aria III flow cytometer (BD) into 96-well plates containing cell lysis buffer. Plates containing FACS-sorted cells were then frozen and kept at -80°C until shipment to ETH Genomic Facility for library preparation and sequencing. Libraries were prepared for sequencing using Nextera XT DNA library preparation kit (Illumina) protocols.
GEO Accession
GSM4280155