16S rRNA sequencing of zebrafish: adult intestine
DNA was extracted from intestinal contents of adult zebrafish in the control group (0.01% DMSO). Then the DNA concentration and purity were quantified. The bacterial 16S rRNA gene was amplified targeting the V3-V4 region using universal primer 338F/806R. The PCR products were normalized and then the libraries were sequenced either on 300 PE MiSeq runs.