Amplicon sequencing of 16S, 18S and rbcL DNA from nutrient enriched sediments in two New Zealand estuaries
Surface sediment from the plots in the nutrient enrichment experiment was collected using a small sampling vial (1.5 cm diam. x 1.5 cm deep). The sample was stored on ice until returning to the lab and freezing at -20C. 0.25g of this sediment was extracted using the DNeasy Powersoil Kit. Seperate PCRs were undertaken using 16S, 18S and rbcL DNA primers. Sequencing was undertaken on an Illumina Miseq