miRNA-Seq of sugarcane drought treated: leaf
Total RNA was extracted from leaves using Trizol reagent (Invitrogen, CA, USA) following the manufacturers procedure. The sRNA librariy was constructed using TruSeq Small RNA Sample Prep Kits (Illumina, San Diego, USA), and then were sequenced with Illumina HiSeq 2500 platform, resulting 50 bp single-end reads.