GSM3168983: YN3; Mus musculus; RNA-Seq
Fifty HSCs were sorted by FACS into wells of a 96-well polymerase chain reaction plate containing lysis buffer, and amplify the cell sample directly with the Smart-Seq2 method. (1) amplified products cDNA was used as input for the library construction of single cell transcriptome. (2) Fragment the sample cDNA into 300bp by Bioruptor®Sonication System. (3) Perform terminal repair, addition of A, and ligation of sequencing adapters to the fragmented cDNA. Amplify the sequencing adapter products by PCR and distinguish each sample by adding different index tags. (4) Perform electrophoresis with the PCR amplification products in 2% agarose gels and extract the 350~450bp DNA from the gel through CWBIO Gel Extraction Kit. Dissolve the DNA for the final library.
GEO Accession
GSM3168983