Illumina HiSeq 2000 sequencing; Xanthomonas campestris pv. campestris (8004) far-red light irradiation and XccBphP overexpression transcriptional analysis
Xanthomonas campestris pv. campestris (8004) far-red light irradiation and XccBphP overexpression transcriptional analysis
28°C in PYM broth, 250 rpm, 16 h, harvest at logarithmic phase (0.7–0.8 OD600)  bacterial far-red irradiation / dark-adaptation MasterPureTM RNA Purification Kit (Epicentre, Illumina) rRNA removal with Ribo-Zero (Illumina) and TruSeq RNA-seq library preparation
Experimental Factor: far-red
stimulus
Experimental Factor: XccbphP::Spcr + pXccBphP
genotype