GSM1960344: GFP rep3; Homo sapiens; RNA-Seq
Cell lysates were obtained by adding RLT solution (Qiagen) to monolayer cells and RNA was isolated using RNeasy columns followed by DNase I digestion. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA-seq libraries were prepared using the TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat Sample Preparation kit (Illumina) and sequenced using the 100 bp paired-end protocol on an Illumina HiSeq 2000.
GEO Accession
GSM1960344