Illumina HiSeq 2500 sequencing of algae: concentrated alage
Algae culture was centrifuged in 1.5 ml sterile centrifugal tubes at 800g for 5 min, and precipitation was subsequently collected. Total DNA was used as the template for PCR amplication of bacterial 16S rRNA gene, using an universal primer set 515F and 806R targeting the V4 region.