GSM3692299: pCre-mTm-R3; Mus musculus; RNA-Seq
Total RNA was isolated with Trizol, with an extra chloroform extraction to remove residual phenol and addition of glyco-blue as a carrier to promote RNA precipitation. Diretional RNA-seq libraries were prepared from 25ng total RNA using the NEBNext Directional Ultra II RNA Library Prep Kit for Illumina (New England Biolabs), with initial polyA+ isolation.
GEO Accession
GSM3692299