GSM2338032: MDA-MB-231 nuclear matrix biological replicate 1; Homo sapiens; OTHER
DNA was isolated by extraction using phenol:chloroform:isoamyl alcohol (25:24:1) followed by chloroform:isoamyl alcohol (24:1) and RNA was digested by incubating with 200 μg/mL RNase A for 2h at 37°C. RNase was removed with a second phenol:chloroform:isoamyl alcohol/ chloroform:isoamyl alcohol extraction series and DNA was treated with 200 μg/mL Proteinase K for 2h at 55°C. Samples were then extracted a third time with phenol:chloroform:isoamyl alcohol, followed by chloroform:isoamyl alcohol, and precipitated overnight at –20°C in 100% ethanol MAR-Seq DNA was quantified, fragmented by sonication, and converted into libraries for deep sequencing on the Illumina Hi-Seq 2000 platform, following the Illumina TruSeq DNA Sample Preparation v2 Guide.
GEO Accession
GSM2338032