GSM2149076: RNASeq WT 1; Schizosaccharomyces pombe; ncRNA-Seq
total RNA extraction by acid phenol protocol total RNA was quantified by Qubit fluorometer using the Quant-iT RNA Assay Kit (Life Technologies). Using Illumina’s small RNA Sample Prep Kit v1.5, 3’ and 5’ adapters were ligated to 5 µg of total RNA, followed by reverse transcription and 12 cycles of amplification. Amplification products of 125 to 250 nucleotides size selected from a 4% agarose gel and purified. Libraries were quantified by Qubit fluorometer using the Quant-iT DNA HS Assay Kit (Life Technologies). Using Illumina’s Cluster Generation Kit, libraries were subject to cluster generation at 8 pM concentration as one sample per lane. Sequencing-by-synthesis was performed for 38 cycles. Strand-specific RNA-Seq
GEO Accession
GSM2149076