EUfall14_Sarracenia_purpurea_rosea_5
Collections of pitcher plant fluid were made from an outdoor common garden from 15 species across a two year period sampling both in the spring and fall. DNA extractions followed the Lodhi et al. (1994) protocol with two separate chloroform:iso-Amyl alcohol cleanings. Each sample was treated with RNAase A. The V9 hypervariable region on the 18S rRNA gene was targeted using a eukaryotic-specific primer pair [Euk1391F: 5'-GTACACACCGCCCGTC-3' (Stoeck et al., 2010) and EukB: 5'-TGATCCTTCTGCAGGTTCACCTAC-3' (Medlin et al., 1988).