GSM4626732: MM360; Mus musculus; RNA-Seq
Total RNA was isolated from mouse hypothalami using Qiazol lysis reagent followed by purification with the RNeasy Mini kit (Qiagen) according to manufacturer protocol. Genomic DNA contamination was removed with the DNA-free kit (Ambion) and remaining DNA-free RNA was tested for purity using PCR for genomic locus. Total RNA was tested on the 2100 Bioanalyzer (Agilent Technologies) to ensure a RIN quality higher than 9, and quantified using Nanodrop. Ribosomal RNA was depleted using the RiboErasemodule (Roche)followed by library preparation using KAPA RNA HyperPrep Kit(Roche) The libraries were sequenced as 50bp pair-end readsusing Nova-seq Illumina platform.
GEO Accession
GSM4626732