GSM1960528: GAB2_WT_C; Mus musculus; RNA-Seq
RNA (150 ng) from each sample was reverse transcribed into cDNA by using the Illumina TruSeq Stranded mRNA kit. cDNA libraries were size-indexed and validated using an Agilent Bioanalyzer, and their concentrations confirmed by qPCR. Six different libraries (3 WT-Gab2+/+ and 3 Gab2-/-) were normalized to 10 nM and pooled. Pooled libraries (8 pmol each) were loaded onto Illumina cBot for cluster generation, and the flow cell was subjected to 100 cycles of paired-end sequencing on an Illumina HiSeq 2000.
GEO Accession
GSM1960528