Intrinsic RNA-Binding Preferences of Eukaryotic Translation Initiation Factor eIF4G Contribute to Competitive Discrimination of Different mRNAs
T7-transcribed pool contains RT primer binding site RT was performed with SSIII cDNA was circularized with circ ligase PCR performed with Phusion RNA polymerase  The following multiplexing barcodes were added during PCR: AGGTTT 0 nM eIF4G1 CCTGAG 10 nM GAACCC 20 nM  TTCAGA 40 nM TGCTGT 80 nM ACGGTG 160 nM CATCAC 320 nM GTAACA 640 nM GGATCT 1280 nM TCCGGG input  [0, 10, 20, 40, 80, 160, 320, 640, 1280, 0]