Pacbio sequencing of Treponema pallidum tprK amplicon
The region of interest was amplified using a HiFi polymerase (Takara). The resulting PCR product was purified with 0.6x volumes of AMPure XP Beads (Beckman Coulter) and diluted to 1ng/ul. The library was prepared and sequenced on a Sequel I SMRT Cell 1M with a 10 hour movie by the University of Washington Pacbio Sequencing Services.