SM2 - biological replicate 1
RNA was extracted with Qiagen’s RNeasy micro kit from 2-3 X104 FACS isolated cells as per instructions. For generation of sequencing libraries, 25ng of RNA (RIN value >9) were submitted to SPIA amplification (NuGen). Two biological replicates per condition were sequenced using the HiSeq 2000 sequencing platform (Illumina, San Diego, CA, USA). Each library was pair-end with a 100nt read length (350nt average insert size). The targeted number of sequencing reads per sample was 15 million.