MeDIP-seq on gDNA from HT1080 cells expressing Mhy1 and Mhy2
DNA was fragmented using a Misonix S-4000 sonicator (Qsonica), equipped with a cup horn. “A” bases were added to the 3’ end following the addition of ligating adapters to repair the fragmented DNA samples according to the Illumina genomic DNA preparation protocol. 200-250 bp DNA fragments were purified from a low-range Ultra Agarose gel. Immunoprecipitation using a Methylated-DNA IP kit (Zymo Research) was used to separate the methylated DNA from the non-methylated DNA. Both resulting methylated and non-methylated samples were subjected to the PCRl enrichment using specific primers (Illumina). The final methylated and non-methylated DNA libraries were sequenced using a Illumina GA IIx DNA sequencer.