16s amplicon sequencing of aphids from pepper crops
The amplification of the 16S rRNA gene was carried out using the universal primers Bakt_341F (CCTACGGGNGGCWGCAG) and Bakt_805R (GACTACHVGGGTATCTAATCC), which amplify the V3 and V4 hypervariable regions. Library was donde using the Herculase II Fusion DNA Plymerase Nextera XT Index Kit V2. The sequencing was obtained on a MiSeq Illumina platform generating 300-bp paired reads. The entire amplification and sequencing process was performed by Macrogen Corp.