GSM2578992: RNASeq_Htz1_4KR_caffeine-120-min_rep2; Saccharomyces cerevisiae; RNA-Seq
Cultures were crosslinked with 1% (v/v) formaldehyde, quenched with glycine, and cells were lysed with beads in a FastPrep-24 (MP Biomedicals) cell homogeniser. Sonicated, clarified extracts were immunoprecipitated with anti-Htz1 antibodies before washing and DNA extraction, or were used directly to prepare input DNA. Illumina TruSeq ChIP Sample Prep Kit were used for ChIP-seq library preparation. RNA library was prepared using the Illumina TruSeq stranded mRNA sample preparation kit.
GEO Accession
GSM2578992