Rad-seq of S. lautus: Dune F8 survivors, replicate A
We sampled plants growing in the alpine meadows. We extracted DNAs from each plant individually using a CTAB protocol. DNAs were quantified with the Pico-green reagent and mixed at equimolar ratios according to their replicate. Pooled DNAs were cleaned using Promega's WizardŹ DNA Clean-Up System and processed into RAD libraries at Floragenex following Baird et al. (2008), except that the methylation-sensitive restriction nuclease PstI was employed. Genomic libraries were sequenced with the Illumina HiSeq 2000 following Illumina/Solexa protocols for single read (1x100 bp) sequencing chemistry.