Sample 2 from solid part of grass and pig manure co-digestion
Sample "Biograss_Solid_Fraction_FG2" was collected from steady state reactors digesting swine manure and pretreated ensiled meadow grass. Approximately 3 g of wet grass and fibers with firmly adherent bacteria were separated from the liquid phase using a sterile net and washed intensively with cold sterile BA medium to remove unattached and loosely adherent microbes. The genomic DNA was processed according to PowerSoil® DNA Isolation Kit protocol. DNA libraries were prepared with Nextera XT kit (Illumina, San Diego, CA) and samples were sequenced using NextSeq 500 sequencing technology producing 150 bp paired end reads.