Leaf Drought Stress biological replicate 1 from E. globulus
The cDNA library construction and sequencing were made by ArrayXpress Inc. (http://www.arrayxpress.com). Total RNA was isolated from all samples acording to the CTAB RNA isolation procedure. The RNA was then passed through a Qiagn RNeasy MinElute Cleanup to remove an residual impurities. RNA concentration was determined by Qubit RNA HS assay. RNA integrity, quality and quality were assessed using an Agilent 2100 BioAnalyzer and the Eukaryote Total RNA Nano Assay. The 18 RNA-Seq libraries were constructed from 1 ug total RNA using the NEBNext Ultra Directional RNA library prep kit for Illumina and indexed with Indexing Oligos Kit Set 2. The quality and size distribution of the final sequences libraries were assessed using an Agilent 2100 BioAnalyzer and the DNA High Sensitivity Kit Assay. Libraries were sequenced for one full flow cell of Illumina single read HiSeq next generation sequencing (2 lanes per pooled library).