RNA-Seq of Coturnix japonica 15 months male Kidney. Quail inbred line Cons DD (INRA), individual 7356, used to generate a reference genome assembly.
Libraries were generated using the Illumina TruSeq stranded mRNA kit. mRNA were selected using poly-T beads, then they were fragmented to generate double stranded cDNA and adaptators were ligated to be sequenced. 10 cycles of PCR were applied to amplify libraries.