GSM4226915: Nic-free_rep2; Mus musculus; RNA-Seq
RNA isolation was performed using TRIzol reagent (Thermo Fisher Scientific) in accordance with the manufacturer's instruction, and RNA purity and concentration was determined via NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific). Only samples with a 260/280 wavelength ratio between 1.8 and 2.0 was used in subsequent sequencing steps. RNA-seq libraries were prepared with single-end Illumina Hi-Seq2000 80 nt strand-specific
GEO Accession
GSM4226915