iPSC-CVPC RNA: Sample eee96072-e740-4967-ba17-c9178796fde1
We isolated total RNA from total-cell lysates using the Quick-RNA™ MiniPrep Kit (Zymo Research) from frozen total-cell lysate material pellets, including on-column DNAse treatment steps and eluted in 60 µl RNAse-free water. RNA elutions were run on a Bioanalyzer (Agilent) to determine integrity and all samples had RNA integrity number (RIN) values greater than 9. Illumina Truseq Stranded mRNA libraries were prepared and sequenced on HiSeq4000, to an average of 28 M 125 bp paired-end reads per sample. 
alignment_software
STAR