GSM3937216: Lin-CD45-Cd71- Flushed, non-digested; Mus musculus; RNA-Seq
Fresh bone marrow cells were isolated by flushing of long bones. Cells were stained with fluorescently labelled antibodies against lineage markers (CD4, CD8, CD11b, B220, Gr1, Ter119), CD71 and CD45. A DNA dye was used to exclude debris. Single Lin-CD45-CD71-DNA+ cells were sorted into PBS-2%FCS. Full-length cDNA from individual cells were produced following the Chromium Single Cell 3' Reagent v2 protocol (10x genomics, Pleasanton, CA) according to manufacturer's recommendations. Default library production protocol, 10x genomics, Pleasanton, CA