RNA-seq of P. melleum plasmodia
Plasmodia were cultured on oatmeal agar and incubated in controlled growth chambers at 23C. Total RNA was extracted using TRIzon Reagent and the integrity of RNA was checked with Agilent Bioanalyser 2100. All samples were sent to Realbio Genomics Institute (Shanghai, China) for library construction and Illumina sequencing. Sequencing was performed on an Illumina Hiseq 2500 platform to generate 150 bp paired-end (PE) reads