GSM3557510: Passage9 (bulk RNA-seq); Homo sapiens; RNA-Seq
Total RNA extractions were performed on samples from passages 2, 3, 4, 6, 8, 9, and 10 using RNeasy Mini Kit (Qiagen) according to the manufacturer's protocol. RNA was quantified using Nanodrop 8000 (Thermo Scientific, Wilmington, DE). Total RNA quality was evaluated following isolation using a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).  Samples with an RNA Integrity Number (RIN) value ≥ 8 were used for bulk cell RNA-Seq and MicroArray for gene expression analysis. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM3557510