GSM3692012: control_infected_300cfu_rep2; Danio rerio; RNA-Seq
Pools of embryos for RNA isolation were snap frozen in liquid nitrogen and subsequently stored at -80°C. Embryos were homogenized in 0,7 ml of QIAzol® Reagent (Qiagen) and subsequently total RNA was extracted and on-column DNase digestion was performed with a RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. The integrity of the RNA was confirmed by Lab-on-chip analysis using the 2100 Bioanalyzer (Agilent Technologies). A total of 0.5 μg of RNA was used to make RNAseq libraries using the Illumina TruSeq Stranded mRNA Library Prep Kit (Illumina Inc., San Diego, USA). The resulting mRNA-Seq library was sequenced using an Illumina HiSeq2500 instrument according to the manufacturer's description with a read length of 1 x 50 nucleotides.
GEO Accession
GSM3692012