16S amplicon sequencing of rock powder amended soil: control amended plot, sample 20
The V4 region of the 16S rRNA gene was amplified from soil metagenomic DNA template with the primers 515f (5-GTGYCAGCMGCCGCGGTAA-3)and 806rB (5-GGACTACNVGGGTWTCTAAT-3) according to the Earth Microbiome project protocol (http://www.earthmicrobiome.org/emp-standard-protocols/16s/). The resultant amplicons were sequenced on an Illumina MiSeq instrument using a 600 cycle kit version 3. The sequence reads were demultiplexed at the time of sequencing using the Illumina software.