GSM3168525: 35S:SUC2_H3K23Ac_ChIP_rep2; Arabidopsis thaliana; ChIP-Seq
Five grams of seedlings were cross-linked with 1% formaldehyde in PBS buffer and ground in liquid nitrogen. Sonicated chromatin was incubated overnight with anti-H2A.Z3, anti-H3K18ac (ab1191, Abcam), anti-H3K23ac (07-355, Millipore), anti-MYC (05-724, Millipore), anti-FLAG (F1804, Sigma). Immunoprecipitated DNA was treated with RNase A (Qiagen) and purified with the QIAquick purification kit (Qiagen). For individual locus detection, ChIP products were diluted with 80 μL of TE buffer and inputs were diluted with TE buffer at the ratio of 1:100
GEO Accession
GSM3168525