GSM4230272: SPDH_transfected_chicken; Gallus gallus; ChIP-Seq
Nuclei were isolated with three different lysis buffers as in Lee et al., 2006 and sonicated in the lysis buffer 3 until the chromatin was disrupted to the size between 200 and 300bp. Antibody: FLAG (Sigma Mouse monoclonal M2; F3165) Libraries were prepared using NEBNext ChIP-seq Library Prep Master Mix for Illumina (New England Biolabs), fragments between 300-450bp were selected and sequenced on a Illumina HiSeq 1500 using Illumina TruSeq v3 chemistry.
GEO Accession
GSM4230272