GSM2677415: CN_1; Mus musculus; RIP-Seq
Due to the small quantity of tissue, both optic nerves from two mice within each group (EAE or control) were pooled to create each sample. Optic nerves were dissected out and rapidly snap frozen in liquid nitrogen. After RNA co-immunoprecipitation process, RNA was isolated using Direct-zol RNA microPrep (Zymo Research) . Double-stranded cDNA was generated using a mixture of random hexamers and oligo dT with the Nugen Ovation RNA-Seq System V2 kit and the sequencing library was made using the Kapa LTP library kit.
GEO Accession
GSM2677415