GSM4189316: CC040_lung_rna; Mus musculus; RNA-Seq
Mice were euthanized by exsanguination following deep nembutal (100 mg/kg intraperitoneal injection) anesthesia. Lungs, livers, and kidneys were excised, snap-frozen in liquid nitrogen, and stored at –80°C. Total RNA was isolated from flash-frozen tissue samples using a Qiagen miRNeasy Kit (Valencia, CA) according to the manufacturer's protocol RNA purity and integrity were evaluated using a Thermo Scientific Nanodrop 2000 (Waltham, MA) and an Agilent 2100 Bioanalyzer (Santa Clara, CA), respectively. A minimum RNA integrity value of 7.0 was required for RNA samples to be used for library preparation and sequencing. Libraries for samples with a sufficient RNA integrity value were prepared using the Illumina TruSeq Total RNA Sample Prep Kit (Illumina, Inc., San Diego, USA) with ribosomal depletion
GEO Accession
GSM4189316