GSM1961604: mRNA_En1_V1; Mus musculus; RNA-Seq
Isolated cells were collected directly into RNA lysis buffer (miRvana kit) and the total RNA isolation protocol was followed. mRNA sequencing libraries were prepared using the TruSeq RNA Library Preparation Kit (v2) according to the manufacturer’s instructions (Illumina). Briefly, RNA with polyA+ tails was selected using oligo-dT beads. mRNA was then fragmented and reverse-transcribed into cDNA. cDNA was end-repaired, index adapter-ligated and PCR amplified. AMPure XP beads (Beckman Coulter) were used to purify nucleic acids after each step.
GEO Accession
GSM1961604