Rehmannia transcriptome
The rehmannia cDNA library was constructed using a modified SMART cDNA synthesis protocol:a modified poly(T) primer with a BsgI site(5'-AAGCAGTGGTATCAACGCAGAGTACT(20)VN-3') was used in this study instead of the normal poly(T) primer. After the double-strand cDNAs were synthesized by LD-PCR, it was treated with BsgI (NEB, MA, USA) to remove the poly(A/T)tail.