Splinkerette-seq: PBS
Sleeping beauty transposon integration was detected by splinkerette PCR. Briefly, Mouse OT-I;Cas9 CD8+ T cells transduced with AAV-SB-CRISPR were collected for genomic DNA extraction using QIAamp Fast DNA Tissue Kit. A total of 1 g genomic DNA was digested with Sau3AI, adaptors were generated and used for ligation. A nested-PCR reaction was used to amplify transposon arm and its junction genomic DNA sequence. Gel purified PCR products were prepared using a Nextera kit (Illumina) before sequencing. Paired-end FASTQ reads were pre-processed as described in manuscript; trimmed and filtered reads provided here as single-end files.