GSM4278818: RNAKD2: D7 ES Jpx KD RNA-seq, rep2; Mus musculus; RNA-Seq
Total RNA was extracted using TRIzol, depleted of ribosomal RNA and then treated with DNase. The rRNA-depleted RNA was selected by size greater than 200nt, fragmented to a mediam size of about 200nt and then subjected to RT reaction. The subsequent first-strand cDNA was subjected to second strand cDNA synthesis using NEBNext Ultra Directional RNA Second Strand Synthesis Module (NEB, #E7550S).  Double-stranded DNA was subjected to library construction using  NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina.
GEO Accession
GSM4278818