GSM3391552: RPE-Cas9-p53KD_KO2-d4; Homo sapiens; OTHER
genomic DNA was collected by lysing in SDS and treating with proteinase K and RNase A. gDNA was purified with Ethanol precipitation. CRISPR sequencing libraries were created using primers to amplify the sgRNA sequences. The primers encoded adaptors P5 and P7 for use in Illumina sequencing machines.
GEO Accession
GSM3391552