De novo assembly of a tadpole shrimp (Triops newberryi) transcriptome and preliminary differential gene expression analysis
Libraries for a total of six samples (three replicates per water condition) were made from approximately 2 µg RNA using Illumina’s TruSeq RNA Sample Preparation Kit from Illumina. The average insert size of each library was approximately 160 base pairs long. Libraries were sequenced on the Illumina HiSeq 2000 to obtain 2 x 50 base pair (bp) paired-end reads. This experiment represents the third replicate from the non-native condition.