GSM4628142: ATAC-Seq_TS543_shH2AFV#2_rep2; Homo sapiens; ATAC-seq
Cells were harvested and frozen in culture media containing 5% DMSO. Frozen cells were sent to Active Motif to perform the ATAC-seq assay. The cells were then thawed in a 37oC water bath, pelleted, washed with cold PBS, and tagmented as previously described (Buenrostro et al, Nature Methods 2013), with some modifications based on Corces et al., Nature methods, 2017. Briefly, cell pellets were resuspended in lysis buffer, pelleted, and tagmented using the enzyme and buffer provided in the Nextera Library Prep Kit (Illumina). Tagmented DNA was then purified using the MinElute PCR purification kit (Qiagen), amplified with 10 cycles of PCR, and purified. Extracted material was quantified using the KAPA Library Quantification Kit for Illumina platforms (KAPA Biosystems), and sequenced with PE42 sequencing on the NextSeq 500 sequencer (Illumina).
GEO Accession
GSM4628142