GSM2838118: oep_p0_S77; Danio rerio; RNA-Seq
50% epiboly embryos were manually deyolked and mechanically dissociated. Single cells were manually picked under a dissecting microscope and flash frozen on dry ice in 4uL lysis buffer. Libraries were prepared using a SMART-seq2 protocol (Picelli et al., 2014) with custom TSO oligos and indexing adapters (Satija et al., 2015). Reaction volumn for tagmentation steps were reduced to 15% of the volumn indicated in the protocol. 88 MZoep libraries were built with customized indices for 5'-enriched transcripts with unique molecular identifiers (UMIs). The remaining libraries were built without UMIs, and contained fragments along full-length transcripts.