Bulk mRNA-seq: OT-I;Cas9 CD8 T cell transduced with AAV-sgPdia3 virus, replicate 3
Activated OT-I;Cas9 CD8+ T cells were transduced with AAV-sgPdia3 or AAV-vector virus. mRNA library preparation was performed at day 5 after virus transduction using a NEBNext Ultra RNA Library Prep Kit for Illumina. Samples were multiplexed using barcoded primers provided by NEBNext Multiplex Oligos for Illumina (Index Primers Set 1).