GSM2577582: CgPHO4 pho2Δ replicate 2; Saccharomyces cerevisiae; RNA-Seq
20 mL of OD600=0.3-0.5 cell cultures were added directly into 30 mL cold methanol (-50 °C), mixed, and incubated in an ethanol-dry ice bath (-50 °C) for at least 20 minutes. Cells were collected by centrifugation and quickly washed with ice-cold water to remove alcohol, and resuspended in RNAlater solution (Ambion). For each sample, 5×107 cells were used to isolate total RNA with the RNase Mini kit (Qiangen), RNA libraries were prepared using the Illumina TruSeq stranded mRNA library preparation kit (part #RS-122-2101 and #RS-122-2102) following the manufacturer's protocol
GEO Accession
GSM2577582