GSM2150390: Input wt2; Schizosaccharomyces pombe; ChIP-Seq
cells were harvested and lysed with a FastPrep cell disrupter (FP120, Bio101 Thermo Savant). DNA was sheared to a range of 250-400bp with a Bioruptor Standard (Diagenode). For Ser5PolII ChiP-seq we used αPolIIser5 monoclonal antibody (Abcam/ab5131) combined with Dynabeads Protein A (Life Technologies).  After reversing the crosslinks DNA was purified using phenol-chloroform and precipitated with LiCl and isopropanol Illumina TruSeq RNA Sample Prep Kit, 48 SetA Ref: 15013135 Lot: 6103158. Although this is Chip-DNA, the “TruSeq RNA Sample Prep Kit” has been used for Library Preparation
GEO Accession
GSM2150390