GSM2334779: Q25_UUS; Homo sapiens; RNA-Seq
Total RNA was extracted from frozen tissue specimens using the miRNeasy Mini Kit (Qiagen), including DNase treatment using RNase-free DNase Set (Qiagen). RNA quality was verified using Bio-Rad Experion system (Bio-Rad Laboratories). Samples were prepared with the Illumina TruSeq RNA Sample Preparation Kit (Illumina). The insert sizes of the libraries were assessed using Agilent Technologies 2100 Bioanalyzer. Validated DNA libraries were pooled, clustered on the cBot cluster generation station (Illumina), and sequenced on a HiSeq 2000 platform (Illumina). Prepared libraries were sequenced using HiSeq 2000 (Illumina) operated in paired-end 2x100 bp mode. Reads were quality-filtered using a standard Illumina pipeline.
GEO Accession
GSM2334779