GSM3692994: SKOV3_pLVX_2_RNA-Seq; Homo sapiens; RNA-Seq
Total RNA was extracted from three biological replicates from each cell line using TRIzol™ Reagent (Invitrogen, Catalog No. 15596026) second to recommendations from manufacturer. For small RNA cDNA library, we followed the protocol from Farazi et al (Farazi et al., 2012) after RNA extraction. AGO 4SU-PAR-CLIP method was performed as described previously (Benhalevy et al., 2016; Hafner et al., 2010) All the samples were sequenced on an Illumina HiSeq 3000 machine using the 50 cycle single read protocol.
GEO Accession
GSM3692994