Aag2 50-75pM Experiment 2
Bound libraries were sequenced on the PacBio RSII using P6/C4 chemistry (PacBio DNA Sequencing Reagent Kit 4.0 v2), magnetic bead loading (PacBio MagBead kit v2) and 6 hr collection times. 84 SMRTcells (v3) of the > 15 kb library were loaded at concentrations of 75-100 pM on-plate. 32 SMRTcells of the > 17 kb library was prepared separately and loaded at on-plate concentrations of 40 pM and 60 pM. These 116 SMRTcells generated 92.7 GB of sequencing data, which resulted in approximately 76X coverage of the Aag2 genome. Average polymerase read lengths, and average subread lengths were 15.5KB and 13.2 kb, respectively.