GSM3171680: Etv6_ChIP1; Xenopus laevis; ChIP-Seq
Etv6 immunoprecipitated DNA was eluted from beads in a thermomixer at 65 degres for 15 min and the eluates and reserved inputs were reverse cross-linked overnight at 65C. The samples were then treated with RNaseA, proteinase K and phenol/chloroform purified, and quantified using Qubit Fluorometer Sequencing libraries were constructed with 1 ng of DNA using NEBNext Ultra DNA Library Prep Kit (NEB#E7370) according to manufacturer's protocol
GEO Accession
GSM3171680