GSM2756221: LI_Adult1_T2-V-2; Homo sapiens; RNA-Seq
After pick single cells using mouth pipette, they were transferred to cold scRNA-seq lysis buffer immediately. Subsequently, the lysis buffer were shocked for 3min at 72℃ to make sure the release of RNA. We use a modified STRT-Seq protocol to construct our library. In brief, we use single cell-specific barcodes to ensure the protocol was applicable to a large number of cells. After cDNA amplification, the single cells labeled with different barcodes as many as 44-88 cells can be pooled together for subsequent library construction and sequencing. What's more, we add biotin in our indexed primers which can pair with 3' cDNA primers, so after additional amplifying of pooled cDNAs following shearing to about 300bp , we use Dynabeads® MyOne™ Streptavidin C1 beads to enrich for the 3'
GEO Accession
GSM2756221