Illumina HiSeq 4000 sequencing; Single-cell RNA-seq of of bone marrow derived mononuclear phagocytes derived from mouse; rat; rabbit or pig
Single-cell RNA-seq of of bone marrow derived mononuclear phagocytes derived from mouse; rat; rabbit or pig
Cells were washed with PBS and then detached using a non-enzymatic solution.  Cells were stimulated with either 100ng/mL lipopolysaccharide (LPS) (Invivogen, Cat. Code: tlrl-smlps) or with 1ug/mL of  High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic), transfected with 2uL/mL Lipofectamin 2,000, for 2/4/6 hours before collection. Cells were directly processed in a 10X Chromium machine (10X Genomics) according to the manufacturer's protocol (version V2). The Single Cell Gel Bead kit (#120217), Single cell chip kit (#120219) and Single cell library kit (#120218) were used along with 10x GemCode Single Cell Instrument as per manufacturer specifications and manuals (Document # CG00011; Revision B).
Experimental Factor: organism
Rattus norvegicus
Experimental Factor: stimulus
lipopolysaccharide; 100 nanogram per milliliter
Experimental Factor: time
4