GSM4278662: P7_CB_HET_mouse_1 [P7_C1_7_CB]; Mus musculus; RNA-Seq
Total RNA was extracted at three developmental time periods (Embryonic E17.5, postnatal day-7 (P7) and adult (P35) from different brain regions (cerebral cortex (CX), hippocampus (HIP), and cerebellum (CB)) of WT and Cul3+/- mice. The total RNA was extracted using the QIAGEN RNAeasy isolation kit (QIAGEN) following manufacturer's instructions. RNA sequencing was performed using the same input amount of total RNA for each sample. RNA samples were ribodepleted using Ribo-Zero rRNA Removal Kit (Illumina) and library preparation was performed using the TrueSeq Stranded Total RNA kit for Illumina Sequencing according to the manufacturer's instructions. Paired-end RNA sequencing (2x100bp) was performed on an Illumina HiSeq4000 to an average depth of 40M reads per sample. RNA-seq libraries were prepared using standard Illumina protocols
GEO Accession
GSM4278662