Bing_transciptome
RNA samples were quantified and qualified on the Agilent Technologies 2100Bioanalyzer (Santa Clara Ca. USA). cDNA from RNA was sequenced on the Illumina HiSeq4000 platform (San Diego Ca. USA). Raw read files were checked for read and base quality and content with the Qiagen CLC Genomics Workbench (Waltham Ma. USA) – hereafter referred to as CLC. Raw reads were then trimmed merged and assembled in CLC. Assembled contigs were annotated using Blast2GO software from BioBam Bioinformatics (Valencia Spain). Reads per kilobase per million reads (RPKM) were calculated according to (Mortazavi et al.2008).