16S profiling of sample A04, Islet I, replicate "a"
Detailed description of sample preparation and data handling for 16S profiling is provided in Liu, et al., 2013 (mBio 4, e00076). Briefly, barcoded V3-V6 amplicons were generated using broad-coverage fusion PCR primers on 10 μl of DNA extracts in 50 μl PCR reactions. The barcoded amplicons were pooled and sequenced. Resulting pyrosequences were chimera-checked, and demultiplexed.