GSM2150749: Gly RNA-seq; Mus musculus; RNA-Seq
Brain hemispheres harvested from mice were stored in RNALater (Ambion) overnight at 4C and subsequently stored at -20C. Total RNA was isolated from brain hemispheres of 3 mice per treatment group using Trizol/chloroform extraction, followed by spin column purifications (Qiagen RNeasy midi kit) that included DNase (Qiagen) digestions. RNA samples from biological replicates (n=2 or 3) within each treatment were pooled to obtain one sample per treatment group Standard Illumina protocols were used to prepare RNA libraries for sequencing
GEO Accession
GSM2150749