RNA-Seq of muscle: S.parvisquamis
Total RNA of seven Sillago species were extracted using a standard Trizol Reagent Kit following the manufacturers protocol, respectively. The quantitative evaluation of total RNA by using the Agilent 2100 Bioanalyzer. We then purified mRNA by depleted rRNA from total RNA and the remaining mRNA was cleaned three times. Then, we disintegrated the mRNA into appropriately sized fragments and fragmented mRNA was used to construct a cDNA library. Afterwards, added A-tailing and adapter ligation to the double stranded cDNA, respectively. Then, the cDNA libraries were diluted to 10 pM and quantified using Agilent 2100 Bioanalyzer. Finally, the cDNA libraries were sequenced on the Illumina HiSeq 2000 across one lane with paired-end 150 bp.