RNA-Seq of human chordoma of the spine
RNA extracted from FFPE spinal chordoma slides was used in the library kit Illumina TrueSeq RNA Gold, depleting ribosomal RNA with the Ribo-Zero method and enabeling strand selection. cDNA library sequencing was performed by EA-Quintiles on the Illumina HiSeq 2000 platform with 50bp paired end reads with cluster reads varying from 50 to 68 million.