GSM3554479: SW_02; Rattus norvegicus; RNA-Seq
Animals were sacrificed at 11.5 weeks post-SCI or equivalent uninjured age with a ketamine overdose. The transection injury group was sacrificed  8.5 weeks post-SCI. Hearts were arrested in diastole with an injection of 3M KCl. Animals were perfused with PBS supplemented with 20% RNAlater (Ambion, Life Technologies, Carlsbad, CA). Liver tissue was collected and equal amounts of tissue (200 mg) were processed for RNA isolation using Trizol (Qiagen, Germantown, MD) using standard procedures. Total RNA from each sample was isolated with RNeasy Lipid Tissue Mini Kit (Qiagen). Libraries were prepared using the TruSeq Stranded mRNA LT Sample Prep Kit- Set A (Cat# RS-122-2101) and Set B (Cat# RS-122-2102) with poly-A enrichment.
GEO Accession
GSM3554479