GSM2674867: Teratoma (B-03-113-T-R-1); Homo sapiens; RNA-Seq
RNA was exacted using PicoPure RNA isolation kit (Arcturus Bioscience) and QCed using a 2100 Bioanalyzer (Agilent Technologies) RNA libraries were prepared for sequencing using  RNA sample preparation kit v2 (Illumina) according to the manufacturers standard protocol.  polyadenylated RNA was first purified from total RNA was first purified through oligo-dT attached magnetic beads using two rounds of purification. Poly(A) RNA was subsequently fragmented and primed with random hexamers for cDNA synthesis. First strand cDNA synthesis was for 50 min at 42oC using SuperScript II reverse transcriptase. After second strand cDNA synthesis, multiple indexing adapters were ligated, and libraries QCed with 2100 Bioanalyzer (Agilent Technologies), normalized and pooled prior to sequencing.   Libraries were subjected to 101 base pair-end multiplex sequencing on an Illumina HiSeq 2000 in high output mode.  Samples were multiplexed (7 to 8 samples per lane) resulting in an average depth of 58 million reads per sample
GEO Accession
GSM2674867