C023t
RNA were extracted using Qiagen AllPrep kit.RNA purity was checked using the NanoPhotometer? spectrophotometer (IMPLEN, CA, USA). RNA concentration was measured using Qubit? RNA Assay Kit in Qubit?2.0 Flurometer (Life Technologies, CA, USA). RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Sequencing libraries were generated using NEBNext? UltraTM RNA Library Prep Kit for Illumina? (NEB, USA) following manufacturers recommendations and index codes were added to attribute sequences to each sample. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumia) according to the manufacturers instructions. After cluster generation, the library preparations were sequenced on an Illumina Hiseq X Ten platform and 150 bp paired-end reads were generated.