GSM2677334: ViCHC198 treatment MQ_2_1; Homo sapiens; OTHER
Total RNA from each infected cell pool treated by an indicated drug was extracted by the AllPrep DNA/RNA Mini Kit (Qiagen). Total RNA was further proceeded to purify mRNA by the Oligotex mRNA Mini Kit (Qiagen) to perform RT-PCR . 10 µL mRNA was reverse transcribed (ThermoScript RT-PCR System; Invitrogen, 11145-024) with the primer annealing downstream of the T7 promoter incorporated in the genome of HIV. PCR (Phusion polymerase reaction mix; Thermo Fisher Scientific, F530S) was performed with the primers annealing on the Illumina PE1.0 sequence and the T7 promoter in order to measure individual HIV expression. RNA libraries were pooled in the final concentration of 4 nM and sequenced 50 bp single read by the HiSeq2000.
GEO Accession
GSM2677334