Substrate Independent Benthic Sampler (SIBS) for hard & mixed-bottom habitats: a proof of concept study.
DNA extracts from both sediment and floc samples were PCR-amplified for a segment of the V3-V4 region of the 16S ribosomal RNA (16S rRNA) gene (approximately 450 base pairs [bp]) using the forward S-D-Bact-0341-b-S-17: 5-CCT ACG GGN GGC WGC AG-3 and reverse S-D-Bact-0785-a-A-21: 5- GAC TAC HVG GGT ATC TAA TCC-3 primers from Klindworth et al. (2013), modified to include IlluminaTM overhang adaptors