Telomere sequencing of Schizosaccharomyces pombe: WT-2
Genomic DNA (gDNA) libraries were made using the Illumina® TruSeq® Nano DNALibrary Prep kits as follows: S.pombe genomic DNA was fragmented to a 400 bp insert size,blunt-ended, and size selected using sample purification beads. Indexedadapters were ligated to the ends of the DNA fragments and PCR-amplified toenrich for fragments having adapters on both ends. The final purifiedproduct was then quantitated by qPCR before cluster generation and subsequent paired-endsequencing.