GSM2579112: MRC5 TGF-β Replicate 1 [RNA-seq]; Homo sapiens; RNA-Seq
Total RNAs were extracted from MRC5 cells using TRIzol reagent (Invitrogen). Libraries were generated from 500ng of total RNAs using TruSeq Stranded Total RNA kit (Illumina). Libraries were then quantified with KAPA library quantification kit (Kapa biosystems) and pooled. 4nM of this pool were loaded on a high output flowcell and sequenced on a NextSeq 500 platform (Illumina) with 2x75bp paired-end chemistry.
GEO Accession
GSM2579112