iPSC ATAC: Sample 26c6cb3d-7ca1-4051-9bb9-98ffb18f6d46
Frozen nuclear pellets of 5 x 104 cells each were thawed on ice, suspended in 50 uL transposition reaction mix (2.5 uL Tn5 transposase in 1x TD buffer, Illumina Cat# FC-121-1030), and incubated for 30 min at 37C. Reactions were purified using Qiagen MinElute kit, eluted in 10 uL water and amplified using the KAPA real-time library amplification kit (KAPA Biosystems) with barcoded adaptors. PCR reactions were terminated after 10 to 13 cycles and purified using AmPure XP beads (Beckman Coulter). Samples were size selected using SPRIselect beads (Beckman Coulter) to a size range of 150 to 850 kbp and sequenced on an Illumina HiSeq2500 or HiSeq4000 to an average depth of 30 M 100 bp paired end reads.
alignment_software
STAR