GSM1630178: 4C-seq CorticalNeurons-FOS; Mus musculus; OTHER
Samples were fixed usinng 1% formaldehyde. Fixation was stopped by adding Glycine (0.125M). After lysis, soluble chromatin was subjected to restriction endonuclease digestion overnight. First digestion with HindII, second digestion with DpnII. Intra-molecular ligations were performed using T4 DNA ligase (NEB) for four hours at 16C. Decross-linked, purified chromatin was subkjected to RNase digestion and further purified. PCR reactions were performed  using a first primer designed on the viewpoint and a second outer primer designed beside the DpnII site. Both primers contained illumina sequencing adapters and Barcodes for multiplexing. Approximately 16 reactions were performed for each viewpoint to generate complex libraries.
GEO Accession
GSM1630178