ITS-Seq of cherry metagenome: RU
Fungal ITS1 regions (forward primer GGAAGTAAAAGTCGTAACAAGG; reverse primer GCTGCGTTCTTCATCGATGC) were PCR-amplified. Amplicon sequencing libraries were constructed using a TruSeq Nano DNA LT Library Prep Kit for Illumina (NEB, USA) according to the manufacturer's instructions, while index codes were added.