High-throughput sequencing of Escherichia coli
The libraries were prepared using the Nextera XT DNA Library Prep kit (Illumina). The fragment size distribution and concentration of the libraries was checked in the Agilent 2100 Bioanalyzer using the Agilent DNA 1000 Kit.The libraries were quantified with the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific) and pooled in equimolar amounts. The libraries were sequenced in a fraction of an Illumina HiSeq X (PE150) lane.