ATAC-seq maize A63 v04 leaf
ATAC-Seq libraries were generated following nuclei extraction from frozen and grinded whole leaf tissue. Approximately 40,000 nuclei per sample were tagmented in TD buffer with the Nextera TDE1 enzyme (Illumina) and the resulting DNA fragments were amplified using a number of cycles optimized via qPCR assays. Resulting libraries were sequenced on an Illumina instrument (2x50bps).