RNA-Seq of Quercus suber: leaf tissue
RNA-seq libraries preparation and sequencing was performed by Beijing Genomics Institute BGI (Hong-Kong, China). cDNA synthesis and paired-end library were prepared following the protocol of Illumina® TruSeq RNA Library Preparation kit for non-normalized libraries. This sample was used in the preparation of two independent RNA-seq libraries, each sequenced in two independent lanes using the Illumina HiSeqTM 4000 platform with a read length of 100nt, as part of a pool containing a total of four libraries, which also includes library IDs HL8_RNASeq_Innerbark, HL8_RNASeq_Phellem and HL8_RNASeq_Xylem. Raw reads were filtered by the barcode sequence corresponding to this sample, then adaptors were removed, as well as empty reads and low quality sequences.