ITS METAGENOME SEQUENCING
The amplicon with the Illumina adaptors were amplified by using i5 and i7 primers that add multiplexing index sequences as well as common adapters required for cluster generation (P5 and P7) as per the standard Illumina protocol. The amplicon libraries were purified by 1X AMpureXP beads, checked on Agilent DNA1000 chip on Bioanalyzer2100 and quantified by Qubit Fluorometer 2.0 using Qubit dsDNA HS Assay kit (Life Technologies).