GSM1627941: SE_8-TEX; Bacillus amyloliquefaciens subsp. plantarum str. FZB42; RNA-Seq
The bacterial cells were quick-frozen in liquid nitrogen before total RNA preparation. RNAs were isolated with Trizol® agent and glass beading on FastPrep®-24 system. Libraries for Illumina sequencing (HiSeq) of cDNA were constructed by vertis Biotechnology AG, Germany (http://www.vertis-biotech.com/), as described previously for eukaryotic microRNA (Berezikov et al., 2006) but omitting the RNA size-fractionation step prior to cDNA synthesis.
GEO Accession
GSM1627941