Metagenome of Great Bay Estuary sediment: off Nannie Island, NH, USA
Sample was concentrated using the Microcon YM3 column and quantitated using the Qubit Spectrofluorometer. 500 ng of gDNA was fragmented using the S2 Covaris AFA system and the metagenome library was synthesized using 500 ng of the purified gDNA using the TruSeq DNA kit. The resulting library was quantitated using the KAPA qPCR quantitation system and 11 pM of the library was loaded onto a HiSeq version 3 paired end flow cell. The sample was run on its own flow cell lane. Data was transferred to Casava software pipeline for conversion to FASTq format.
Submitted by
Loren A. Launen, Sinéad M.Ní Chadhain, Jarett L. Miller and Stephen H. Jones