RNAseq of cotton: B2mm2 flower bud
The cDNA libraries (from smaller than 2mm, 2mm and 6mm flower buds) were prepared according to the TruSeq Stranded mRNA LT Sample Preparation Kit (Illumina, San Diego, CA, USA), using 1 ?g of total RNA. The size distribution of the libraries was evaluated using the Agilent 2100 BioanalyzerŽ and the High Sensitivity DNA Kit (Agilent, Santa Clara, CA, USA). The accurate quantification of the libraries was obtained using the 7500 Real Time PCR (Applied Biosystems, Foster City, CA, USA) and the KAPA Library Quantification Kit (Kapa Biosystems, Wilmington, MA, USA). Paired-end sequencing (2 × 300 bp) was performed on a MiSeq (Illumina, San Diego, CA, USA) at Microbiology Laboratory/UFRJ.