GSM4230482: H3K27Ac ChIP RPM2 (PB503); Mus musculus; ChIP-Seq
Individual lung tumors from mice were micro-dissected, flash frozen and stored at -80°C until ChIP. Tumors were prepared for ChIP by pulverizing frozen tumors into a fine powder. Samples were resuspended in PBS and DNA was crosslinked in 1% formaldehyde. Fixation was terminated by adding glycine. Chromatin was sheared using a Diagenode Bioruptor and mouse tumor chromatin was immunoprecipitated with anti-MYC or anti-H3K27Ac (Active-Motif, 39133), followed by either anti-rabbit Dyna beads or Protein G Dyna beads. The immunoprecipitated chromatin was purified with the Zymo ChIP DNA Clean and Concentrator kit. Libraries were prerpared using a NEBBext ChIP-Seq Library kit and sequenced on an Illumina HiSeq 2500 (single-end 50 bp reads with a min of 35 million reads per sample).
GEO Accession
GSM4230482