GSM1700696: isw2_rsc8_chd1_B; Saccharomyces cerevisiae; MNase-Seq
In MNase-seq experiments, nuclei were prepared from unfixed yeast cells and then digested with Mnase. Mono-nucleosomal DNA was gel-purified (~150 bp) as described previously (Cole et al. 2012, PMID: 25348398; Ganguli et al. 2014, PMID: 25015381). In ChIP-seq experiments, cells were formaldehyde cross-linked before harvasting. The IP was performed with anti-Rpb3 antibody from Neoclone (cat: WP012, lot: A5081456) The DNA was repaired using the DNA repair kit from New England Biolabs. The repaired DNA was processed for paired-end sequencing according to the Illumina protocol.
GEO Accession
GSM1700696