GSM1527863: PD10_TFC_mES-G45-DKO-6; Mus musculus; Bisulfite-Seq
The genomic DNA of mouse ES cells was extracted with the DNeasy Blood & Tissue Kit (Qiagen). 1 μg genomic DNA plus 0.1% of unmethylated lambda DNA spike-in was digested by 10 U MspI (Fermentas). Then the digested DNA was end repaired and dA tailed with Klenow Fragment. Next, the DNA fragments was ligated with the Illumina TruSeq adapters using T4 DNA ligase (Fermentas) overnight. Size selection of the 200-500 bp DNA fragments were performed on a 2% agarose gel. After that, bisulphite conversion of the library was performed using a MethylCode DNA Methylation Kit (Invitrogen). The bisulphite-converted DNA was amplified by 15 cycles of PCR using PfuTurbo Cx polymerase (Agilent Technologies) and then purified by AMPure XP beads (Beckman).Finally, the libraries were sequenced on an Illumina HiSeq 2000.
GEO Accession
GSM1527863