GSM2071737: dmsoRep2; Homo sapiens; RNA-Seq
Cells were washed in cold PBS, then scraped in TRIzol RNA purification reagent, which was added directly to each plate according to the manufacterers' instructions. Subsequently, RNA was further purified with a Qiagen Rneasy kit using an on-column DNase I digestion. 500ng per sample total RNA was used (measured with a NanoDrop 1000 and Agilent 2100 Bioanalyzer) to construct libraries following the Illumina TruSeq Stranded RNA Sample Preparation Guide, polyA seq (mRNA)
GEO Accession
GSM2071737