454 Reference for Carlton variety (basal plate)
RNA was extracted using a modified CTAB method developed for pine trees (Chang et al, 1993) followed by RNA clean up from Qiagen RNeasy plant mini kit, rRNA was removed using the Ribominus kit from invitrogen. cDNA library was produced using the cDNA Rapid Library Preparation method (Roche) and labelled with MID6 adaptor (Roche).