K1_d110_large_DNA
Microbes from incubations were then  filtered through a 0.2 m filter and these filters were inserted into the  Qiagen DNease Blood and Tissue DNA Isolation Kit. The extracted bacterial DNA  underwent amplicon library preparation and Illumina sequencing of the 16S-V4  rRNA hypervariable region using 16S-V4 515f and 806r primers on a MiSeq  system.