GSM2337229: Brn3bKOE15SN1; Mus musculus; RNA-Seq
Total RNA was extracted by RNeasy (Qiagen). Libraries were constructed using a stranded modification of the Illumina TruSeq mRNA (Brooks, et al. Meth Mol Biol 2012). Each RGC library was single-end sequenced in an independent lane of a GAIIx at a length of 76 bases, whereas brain samples were sequenced with paired-end 125 bases on HiSeq2500.  Fastq files were generated from reads passing chastity filter. Stranded mRNA-seq (dUTP method)
GEO Accession
GSM2337229