Illumina HiSeq 2500 sequencing; enok_rnaseq
enok_rnaseq
Drosophila melanogaster was bred at Stowers Institute.  The enok1 and enok2 mutant alleles originated from Bloomington Stock Center.  Flies were grown on standard media at 25C unless stated otherwise. Larvae were heat shocked twice at 37C for 40 min, with 30 min break at 25C in between, for two consecutive days starting from the second day after hatching.  Females were conditioned with wet yeast for 2-3 days before ovary dissection. Total RNA was isolated by Trizol, followed by DNase treatment. Libraries were poly-A selected and generated using the default directional Illumina protocol, TruSeq Adapters.
Experimental Factor: genetic modification
none
Experimental Factor: cell type
wild type
Experimental Factor: genotype
wild type
LIBRARY_STRAND
first strand