GSM2151218: parg1; Arabidopsis thaliana; miRNA-Seq
RNA was extracted with TRIzol reagent (Life Technologies, USA, 15596018) Small RNA fragments (~15–30 nucleotides) were selected and recovered from 10 µg of total RNAs by 15% urea-polyacrylamide gel electrophoresis. Libraries were made using the NEBNext Small RNA Library Prep kit (NEB, USA) according to manufacturer’s instructions. Briefly, 3' and 5' adapters were ligated sequentially to the small RNAs. Ligated small RNAs were converted to cDNA by reverse transcription followed by PCR amplification for 15 cycles. The barcoded libraries were sequenced in one lane on the Illumina Hiseq2000.
GEO Accession
GSM2151218