Zea mays ssp. mays L. B73 immature tassel RNA-Seq
Immature tassels before emerging were harvested approximately at 60 days after planting (depending on the maturity rate of each line) Samples from three plants of each inbred/line were pooled for homogenization in liquid nitrogen and RNA extraction.  All RNA extractions were conducted using the Qiagen RNeasy kit cat# 74904 (50), according to Qiagen's protocol. Total RNA was eluted twice with 30 ul RNase-free water. The indexed RNA-Seq libraries were prepared using the Illumina protocol outlined in "TruSeq RNA Sample Preparation Guide" (Part# 15008136 Rev. A November 2010). The indexed libraries from one inbred/line were combined and seeded onto one lane of the flowcell. The libraries were sequenced using 110 cycles of chemistry and imaging, resulting in paired-end (PE) sequencing reads with length of 2 x 101 bp.