GSM4278581: inflorescence meristems, GFP_MYB3R4_ChIP-seq_rep1; Arabidopsis thaliana; ChIP-Seq
ChIP was performed as described (Gendrel et al., 2002) with minor modifications. In brief, chromatin was extracted from approximately 1 to 2 g of crosslinked materials. Chromatin was fragmented by sonication using a bioruptor (Diagenode) in lysis buffer (10 mM Tris-HCl [pH 8], 150 mM NaCl, 1 mM EDTA [pH 8], 0.1% deoxycholate, and 1X protease inhibitor cocktail). All ChIPs were performed in a buffer containing 20mM Tris-HCl (pH8), 150mM NaCl, 2mM EDTA, 1% triton X-100 and 1X protease inhibitor cocktail. ChIP were performed using Anti-inflorescence meristems, GFP antibody - ChIP Grade (Abcam, ab290) coupled to a 1/1 mix of protein-A and protein-G Dynabeads (life technologies, 10001D and 10003D). All ChIPs were performed in a buffer containing 10 mM Tris-HCl [pH 8.0], 5 mM EDTA [pH 8.0], 150 mM NaCl, 1% Triton X-100, and 1× protease inhibitor cocktail. The elution was done with elution buffer (1% SDS, 0.1M NaHCO3). The efficiency of ChIP materials was assessed by qPCR in prior to in-house library preparation, using the TruSeq ChIP sample preparation kit (Illumina, IP-202-1012) following the manufacturer's instructions.  Library preparation was performed by Novogene using another method for some of the samples. The libraries were sequenced on Illumina sequencer by Novogene using paired-end 150bp length reads, and on NextSeq500 at the Sainsbury Laboratory Cambridge University using single-end 75 bp length reads .
GEO Accession
GSM4278581