GSM4002581: 16.7 mM glucose mIAPP replicate 3 [RNA-14-3]; Mus musculus; RNA-Seq
Islets were disrupted by homogenization in PBS and resuspended in 1 ml of TRIzol solution. RNA was extracted using the Directzol RNA mini-prep kit according to the manufacturer's protocol, with DNase I treatment in the column to remove genomic DNA contamination (Zymo Research, Irvine, CA, USA). RNA was eluted in 30 μl TE buffer, and the quantity and quality of RNA examined on a MultiNA bioanalyzer (Shimadzu, Tokyo, Japan). A NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Ipswich, MA) was used to enrich mRNA from 1 μg of total RNA. The NEBNext® UltraTM Directional RNA Library Prep Kit for Illumina® was then used to generate barcoded libraries according to the manufacturer's protocol, and these libraries were then quantified on the MultiNA bioanalyzer and pooled to equimolar ratios for sequencing.
GEO Accession
GSM4002581