16S amplicon seq of sand incubated for 22 days, replicate B
Polymerase chain reaction was performed using primer pair Bact_341F/Bact_805R (Herlemann et al., 2011) and HotStarTaq Mastermix (Qiagen, Hilden, Germany) as described previously (Kumar et al., 2018). Amplicons were purified using NucleoSpin Gel & PCR Clean-Up Kit (Macherey-Nagel, Dren, Germany). The NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs, Frankfurt, Germany) was used to prepare libraries for amplicon sequencing, following the manufacturers instructions. Amplicons were purified using AMPure XP Beads (Beckman Coulter, Krefeld, Germany), and amplicon sequencing was then carried out in-house on a MiSeq Illumina platform (Illumina, Eindhoven, The Netherlands) with v3 chemistry.