GSM2579107: AP14.5NSC2; Mus musculus; RNA-Seq
Embryos were dissected and heads rapidly frozen in OCT. Frozen sections were cut onto Leica PEN membrane slides. Location-matched slides were stained and dehydrated for laser capture microdissection with a Leica LMD6500. RNA was prepared using an Arcturus Picopure RNA Isolation Kit with on-column DNase digestion as described by the manufacturer. Library preparation was performed with an initial amplification step from 15 ng of RNA from each of the six samples with the NuGEN Ovation RNA-Seq System v2 to create double stranded cDNA. The Nextera XT DNA Sample Preparation Kit (Illumina) was used to create DNA library templates from the double stranded cDNA as described by the manufacturer. DNA sequencing was performed on an Illumina HiSeq 2500 instrument using standard protocols for paired end 100 bp sequencing.
GEO Accession
GSM2579107