GSM4503859: Non-infected Rep2; Homo sapiens; RNA-Seq
Total RNA from each sample was extracted with miRNeasy Mini Kit (QIAGEN). CAGE libraries were constructed using total RNAs based on the no-amplification non-tagging CAGE libraries for Illumina next-generation sequencers (nAnT-iCAGE) protocol. Briefly, first strand cDNAs were synthesized through reverse transcription using random primers. Subsequently, 5' caps of RNAs were oxidized and biotinylated. Samples were then treated with RNase I to remove single-stranded RNAs. Biotinylated RNA/cDNA molecules were purified using streptavidin magnetic beads. Single-stranded cDNAs were obtained by treating with RNase H and RNase I. After ligation of adapters and second strand synthesis of cDNAs, CAGE libraries were finalized.
GEO Accession
GSM4503859