Illumina whole genome shotgun sequencing of Alpine soil
The DNA was sheared using Covaris E210 (Covaris, USA) with parameters recommended by Illumina (Illumina Inc, USA). The fragments were end repaired using NEBNext Ultra DNA Library Prep Kit for Illumina (New England BioLabs Ltd., UK) according to manufacturers instructions. The adaptor ligation, including the incorporation of sample index barcodes, was performed using the TruSeq DNA HT Sample Prep Kit (Illumina) according to Illumina instructions. The final products were amplified in 8 PCR cycles. The average size of the libraries was 310 bp. The libraries were normalized to 4 nM solutions and pooled prior to sequencing. Paired-end sequencing (2  250 bp) was performed on an Illumina MiSeq sequencer using the MiSeq Reagent kit v2 (Illumina) and following the standard run protocols