GSM3937284: IgG ChIP-seq in NSCs without Rnase; Mus musculus; ChIP-Seq
2X107 NSCs were harvested, washed and cross-linked with 4% PFA for sonication. Immunoprecipitation was performed with 4 μg rabbit or goat anti-Ars2 antibody. Normal rabbit or goat IgG was used as control. For ChIP with RNase treatment, RNase A (Roche 11579681001) was added to cell pellet and/or chromatin extract to 100 μg/ml. DNA libraries were made using the Illumina ChIP-seq library preparation kit and sequenced as individual lanes on the Illumina GAII.
GEO Accession
GSM3937284