GSM4229104: T69a; Mus musculus; RNA-Seq
Media was removed from Matrigel domes, and 4-6 wells of organoids were harvested in 1 mL total of Trizol (15596-018; Thermo Fisher Scientific). RNA was extracted using the PureLink RNA Mini Kit (12183018A; Thermo Fisher Scientific)  per manufacturer's instructions. RNA quality was assessed on a 2100 Bioanalyzer Instrument (Agilent) using the RNA 6000 Nano Kit (5067-1511; Agilent). Only RNAs with RIN values >9 were used for RNA-sequencing. Sequencing libraries were generated from 1 ug of total RNA using a TruSeq Stranded Total RNA Kit with Ribo-zero human/mouse/rat (RS-122-2201 and RS-122-2202; Illumina) per manufacturer's instructions. Libraries were quality checked and quantified using a Bioanalyzer 2100 (Agilent) with a DNA 1000 Kit (5067-4626; Agilent).
GEO Accession
GSM4229104