GSM4227505: AH3; Pseudomonas aeruginosa; RNA-Seq
Cell pellets were recovered (5000 g, 15 min) and lysed in QIAzol reagent (Qiagen). Total RNA was isolated using the RNeasy mini kit (Qiagen) following manufacturer instructions and DNA removed with DNase I (TURBO DNA-free kit; Invitrogen) following manufacturer instructions. Ribosomal RNA was depleted using the Ribo-Zer rRNA Removal kit (Illumina) following manufacturer instructions. RNA libraries were prepared for sequencing using standard Illumina protocols and the TruSeq stranded mRNA kit (Illumina) and barcoding was then undertaken on RNA for HiSeq analysis. Samples were run on the Illumina HiSeq4000 platform (1X100 base pair paired-end reads).
GEO Accession
GSM4227505