smMIPs of human colorectal cancer patient #5
Up to 500 ng genomic DNA was hybridized with the panel, exonuclease-treated, and PCR-amplified to generate sequencing libraries. Single libraries were prepared from all specimens in this study. Sequencing was performed using the 300 cycle Illumina NextSeq 500/550 High Output v2 kit (Illumina, San Diego, CA, USA).