IgG-seq of Plasma B-cells from GPI-immunized mice Sort3
Single cells were DNA-barcoded from hydrogel beads and IgG transcripts captured using igG heavy chain or light chain gene specific primers creating cDNA. cDNA was PCR amplified using primers specific to particular V-gene families. After paired-end sequencing, reads were quality trimmed, and merged into a single read. Barcode sequences were extracted from the end of the the read and identified. The barcode identifier is added to the FASTQ identifier, but the barcode sequence itself was removed from the merged sequence.