Bragg leaf nodC-
The first trifoliate leaves were harvested from 3 soybean plants after 7 days inoculating with Bradyrhizobium diazoefficiens USDA111?wild-type or nodC- mutant strain. RNA was extracted by the Invitrogen PureLink RNA Mini Kit followed with mRNA isolation using magnetic oligo(dT) beads. The libraries were generated by Illumina TrueSeq kit according to manufacturer procedures.