Whole-genome resequencing of pig, ear
Genomic DNA was extracted from the ear samples using a standard phenolchloroform method and was stored at 4 C to avoid freeze-thawing and tested for concentration (as ng/l) using a Nanodrop. The DNA was fragmented and treated following the Illumina DNA sample preparation protocol, with a process of end repaired, A-tailed, ligated to paired-end adaptors and PCR amplification with 350-bp inserts. The constructed libraries were sequenced on the Illumina HiSeq X Ten platform (Illumina, San Diego, CA) for 150-bp paired-end reads at Novogene (Beijing, China).