Clinical characteristics were refractory GD
mRNA was enriched and cleaved into 300-bp fragments using fragmentation buffer. Then, the fragments were used as templates for double-stranded cDNA (dscDNA) synthesis using the SuperScript dscDNA synthesis kit (Invitrogen, USA) according to the manufacturers instructions. The dscDNA with indexed adapters was purified and enriched by PCR for 15 cycles and then assessed using a TBS-380 fluorometer, a NanoDrop 2000 spectrophotometer, and an Agilent 2100 Bioanalyzer. The prepared libraries were sequenced using BGISEQ-532 sequencer.