WGS of Aspergillus fumigatus: isolate D1 16-6
For each isolate, genomic DNA was extracted using the Qiagen DNeasy Plant Mini Kit as previously described. DNA concentrations were quantified using both the Qubit Fluorometer and Nanodrop spectrophotometer using the manufacturers protocols. Whole genome libraries were prepared and sequenced at the University of Tennessee Health Science Center Molecular Resource Center. Bioinformatics services were provided by code4DNA (www. code4DNA.com). Sequence reads for each sample were aligned using the STAR sequence alignment tool (v2.5.0b) to the A. fumigatus reference sequence (version A_fumigatus_Af293_version_s03-m05-r05) which was downloaded from http://aspgd.org/.