GSM1528810: Mouse_Adult_testes_Asb1_ao36(KO)_sRNA; Mus musculus; ncRNA-Seq
Mouse, rat and rabbit testes were homogenized as described in Lau et al, Science. 2006 Jul 21;313(5785):363-7., and this extract was used only for the Mili (Piwil2) IP experiments.  For total RNA, testes were pulverized into powder while frozen and thawed into Tri-reagent solution to extract RNA according to the manufacterur (MRC) instructions Small RNA libraries were constructed using the custom protocol described in Matts et al. Methods Mol Biol. 2014;1093:123-36.  Messenger RNA libraries were constructed with the Epicentre ScriptSeq v2 kits.
GEO Accession
GSM1528810