Zea mays B73xEDMX-2233 palomero seedling leaf RNA-Seq (Schnable Lab Ac4578; Row #: 11B-872-3)
All RNA extractions were conducted using the Qiagen RNeasy kit cat# 74904 (50), according to Qiagen's protocol. Total RNA was eluted twice with 30 ul RNase-free water. The indexed RNA-Seq libraries were prepared using the Illumina protocol outlined in "TruSeq RNA Sample Preparation Guide" (Part# 15008136 Rev. A November 2010). The indexed libraries from one inbred/line were combined and seeded onto one lane of the flowcell. The libraries were sequenced using 110 cycles of chemistry and imaging, resulting in paired-end (PE) sequencing reads with length of 2 x 101 bp.