GSM3168449: human neuroblastoma cell line SHSY5Y, replicate 2; Homo sapiens; RNA-Seq
Total RNA was isolated from three, separate T25 flasks of adherent SHSY5Y cells at approximately 60% confluency using Sigma's GenElute mammalian Total RNA miniprep kit (Millipore Sigma, RTN70) per the manufacturer's recommendations. Total RNA was quantified on an Agilent Bioanalyzer 2100 using a Eukaryote Total RNA Nano chip (Agilent, 5067-1511) as well as a Nanodrop spectrophotometer (ThermoFisher Scientific, ND-1000). Libraries were made from 500ng of total RNA according to the manufacturer's directions for the TruSeq Stranded mRNA LT– set A and B (Illumina, Cat. No. RS-122-2101 and RS-122-2102) kit. Resulting short fragment libraries were checked for quality and quantity using the Bioanalyzer High Sensitivity DNA assay  (Agilent; 5067-4626) and Qubit Fluorometer (Life Technologies).
GEO Accession
GSM3168449