GSM2754191: ATACseq_SCC3_Tgfbr2KO_A; Mus musculus; ATAC-seq
50,000  TPCs were isolated by FACS and washed once with cold 1x PBS. Cells were pelleted and lysed in 50 μl and spun at 500g for 6min at 4C to collect nuclei. Pelleted nuclei were incubated for 30min at 37 C with Tn5 transposase (Illumina Cat #FC-121-1030) before transposed DNA fragments were purified with a Qiagen MinElute Kit for ATAC-seq library generation.
GEO Accession
GSM2754191