GSM4626494: CAP+CM rep1; Cryptococcus neoformans; RNA-Seq
Cells were pelleted, flash frozen in liquid nitrogen, and lyophilized. Lyophilized cells were resuspended in  TRIzol (ThermoFisher Scientific catalog no. 15596026) and vortexed with a mixture of 1-mm and 0.5mm zirconium beads (BioSpec Products catalog no. 11079110z and 11079105z) in a Biospec Products mini bead beater to lyse. RNA was isolated from the lysate using phenol-chloroform extraction, followed by Qiagen RNeasy column-based cleanup. Libraries were constructed with the NEBNext Ultra II Directional RNA Library Prep with rRNA Depletion Kit (New England BioLabs catalog no. E7760)
GEO Accession
GSM4626494