GSM4566105: Wt1CreERT2:Rosa26mTmG_3; Mus musculus; RNA-Seq
Whole heart was digested with Collagenase II through  the Langendorff perfusion system. The isolated non-cardiac myocytes fraction was subjected to the Fluorescence Activated Cell Sorting system to generate the EGFP positive epicardial derived cells. Single vialbe EGFP positive cell was loaded onto the 10X Genomics Chromium Single cell Gene expression Solution at the density recommended by the manufacturer and barcoded according to the standard protocol. Libraries were sequenced on an Illumina platform using 75-bp pair-end sequencing on a NovaSeq6000 instrument
GEO Accession
GSM4566105