GSM4230123: siLhx4 2; Rattus norvegicus; RNA-Seq
RNA was extracted using TriZOL (Life Technologies) and purified with a Qiagen RNeasyR Micro Kit including DNase I treatment (Qiagen). RNA quality was determined on a Bioanalyzer 2100 (Agilent) with RIN‐values ranging from 7.7 to 8.9. cDNA library preparation with ribosomal RNA depletion was performed with the SMARTer Stranded Total RNASeq Pico Input Mammalian Kit (Takara Bio) using 3.4 ng total RNA. Nine bar coded libraries were pooled and sequenced on one Flow Cell of a HiSeq2500 (Illumina) in Rapid Run Mode.
GEO Accession
GSM4230123