RNA-Seq of Ocimum basilicum: 6Day Water Control
Frozen leaves were individually pulverized with a mortar and pestle using liquid nitrogen.  Powdered tissue was transferred to a 1.5 ml centrifuge tube containing 1 ml of Trizol (Invitrogen, Carlsbad, CA).  RNA was extracted using the Trizol Plus RNA Purification Kit (Invitrogen).  Samples were treated with DNAse (Qiagen, Hilden, Germany) and purified using the GeneJet RNA Cleanup Kit (Thermo Fisher Scientific, Waltham, MA).  RNA quality and quantity was assessed using a Nanodrop 2000 (Thermo Fisher Scientific) and a Tapestation 4200 (Agilent Technologies, Santa Clara, CA).  All RNA samples had a RIN value > 6.0. All RNA samples were converted to cDNA, purified for mRNA, and each was sequenced on an Illumina HiSeq or NovaSeq instrument using a paired end 2 x150 bp format (GENEWIZ, Plainfield, NJ).