GSM2416795: SYNAP_Control_9; Mus musculus; RIP-Seq
Hippocampai from 6 control and 6 status epilepticus mice were extracted, flash frozen on dry ice and stored at -80 degrees celcius until further processing. Control hippocampai and SE hippocampi were combined and synaptoneurosomes and cytoplasm were isolated from each pool. Upf1-bound mRNA was isolated from each sample using a Upf1 antibody (abcam ab109363). Co-precipitated RNA was isolated using Trizol. rRNA was removed from the co-precipitated RNA using the Ribo-Zero Magnetic Kit  (Illumina) RNA libraries were prepared for sequencing according the protocol provided in the Illumina ScriptSeqTM v2 RNA-Seq Library Preparation Kit.
GEO Accession
GSM2416795