GSM2072050: hct116_gegfp_a02_dmso_r2; Homo sapiens; RNA-Seq
Total RNA was harvested using a  RNeasyMini kit (Qiagen 74104) First strand cDNA was generated from 1.5 μg of total RNA using Oligo(dT)12-19 Primer (Invitrogen) and AfifinityScript Multiple Temperature Reverse Transcriptase (Aglient). Second strand cDNA was synthesized using an mRNA Second Strand Synthesis Module (#6111L). Libraries were prepared by tagmentation (Nextera XT DNA Sample Preparation Kit, Illumina) according to the manufacturer’s protocol, using index primers (Nextera XT Index kit, Illumina) to facilitate multiplexing. The concentration of each cDNA library was quantified with the KAPA Illumina ABI Quantification Kit (Kapa Biosystems). Libraries were normalized to 5 nM and pooled for sequencing
GEO Accession
GSM2072050