WGS of mus musculus: adult female spleen CRISPR-treated
Whole genome sequencing (WGS) libraries were prepared using the Truseq DNA PCR-free Library Preparation Kit in accordance with the manufacturerŐs instructions. Briefly, 1ug of DNA was sheared using a Covaris LE220 sonicator (adaptive focused acoustics). DNA fragments underwent bead-based size selection and were subsequently end-repaired, adenylated, and ligated to Illumina sequencing adapters. Final libraries were evaluated using fluorescent-based assays including qPCR with the Universal KAPA Library Quantification Kit and Fragment Analyzer (Advanced Analytics) or BioAnalyzer (Agilent2100). Libraries were sequenced on an Illumina HiSeq 2500 sequencer (v4 chemistry) using 2 x 125bp cycles to a mean coverage of 50x for CRISPR-treated animals and 30x for the control animal