RNA-Seq of Oncorhynchus kisutch : pooled nontransgenic parr liver
12 different non-transgenic large-sized fish were pooled, technical replicate #1. Two technical replicates were submitted to BGI for each sample group. Polyadenylated mRNA was enriched with with oligo0DT magnetic bedas, fragmented to 200 bp length and reverse transcribed with first-strand cDNA synthesis. Double stranded cDNA library was then size-selected and amplified by PCR, and subjected to 2X 50bp single-end sequencing on the Illumina HiSeq2000 platform.