GSM4627279: N2_Day12_Rep2; Caenorhabditis elegans; OTHER
Following lysis, samples were digested with RNase I. Ribosomal pellets were isolated by ultracentrifugation using a sucrose gradient. RNA was extracted using hot phenol/chloroform. RNA fragments in the range of ~20-35 nucleotides were gel purified. After dephosphorylation, a DNA linker (CTGTAGGCACCATCAAT) was ligated to the 3' end of the RNA footprints. Samples were then depleted of rRNA using Ribo-Zero kits (Illumina), followed by reverse transcription into cDNA, and circularization. Libraries were barcoded during PCR amplification and sent for sequencing.
GEO Accession
GSM4627279