Illumina HiSeq 2500 paired end sequencing; RNA-seq of C17.2 neuronal progenitor cells exposed to cerium oxide nanoparticles during cell differentiation
RNA-seq of C17.2 neuronal progenitor cells exposed to cerium oxide nanoparticles during cell differentiation
For differentiation experiments the cells were seeded at a density of 2000 cells/cm2 in suitable cell culture plates in DMEM complete cell medium. After 24 h the cell medium was replaced with differentiation medium consisting of DMEM:F12 medium (Gibco) supplemented with 2 mM glutamine (Gibco), 100 U penicillin/mL, 100 U streptomycin/mL (Gibco) and N2 supplement (1:100) (ThermoFischer Scientific). Half of this differentiation medium was changed every 3 days during differentiation. For RNA extraction, the cells were washed after exposure and total RNA was extracted using the RNeasy Mini Columns (Qiagen) according to the manufacturers instructions, including the purification step with DNase I treatment.  Stranded mRNA-seq (Illumina TruSeq RNA HT with polyA-selection
Experimental Factor: samarium doped  cerium oxide nanoparticles
compound
Experimental Factor: 25
dose
Experimental Factor: 4 day differentiated
developmental stage