mesocosm from grassland soil, heavy (13C labelled) DNA
Libraries of 16S rDNA amplicons were prepared using universal primers 27F (5' – AGA GTT TGA TCC TGG CTC AG - 3') and 533R (5' – TTA CCG CGG CTG CTG GCA C - 3'), extended at the 5’ end by 10 bp multiplex identifiers (MIDs, Roche). As the two primers used for sequencing are tagged by a same MID, it was expected that approximately half of the sequences obtained were sequenced from each primer.