16s honey bee worker: mouthparts, ileum & rectum
The V6-V8 variable region of the 16S rRNA gene was amplified using PCR primers 799F (acCMGGATTAGATACCCKG + barcode) and bac1193R (CRTCCMCACCTTCCTC). Amplification was performed using the HotStarTaq Plus Master Mix Kit (Qiagen, USA) under the following conditions: 94°C for 3 minutes, followed by 28 cycles of 94°C for 30 seconds, 53°C for 40 seconds and 72°C for 1 minute, with a final elongation step at 72°C for 5 minutes.  PCR products were confirmed using a 2% agarose gel. PCR products were used to prepare DNA libraries following Illumina TruSeq DNA library preparation protocol