GSM4677760: sc_m1_5; Mus musculus; RNA-Seq
Isolation of clumps was performed incubated 20min the Jejunum in a 10mM EDTA solution. Afterwards, the tissue was cut in 1cm pieces , moved in a pre-warmed 10 mM EDTA solution for 5 min and shacked vigorously at the end of the incubation time. – To obtain single cell suspension, differently from the clumps protocol, the tissue was incubated for 10 min on ice in a 10mM EDTA solution, before to be cut in small pieces and moved for other 10 min in a pre-warmed 10 mM EDTA solution. The tissue was shacked vigorously every 2 min. for scRNAseq, 3' end mRNA libraries were prepared for sequencing using MARS-seq 2.0 (Keren-Shaul et al, Nature Protocol 2019).
GEO Accession
GSM4677760