GSM4279844: scRNA_MEF_batch7; Mus musculus; RNA-Seq
Individual cell was manually picked after digestion, lysed and subjected to cDNA synthesis. Single-cell cDNA was then amplified and fragmented as published steps. The sequencing library was constructed and sequenced with paired-end 150-bp reads on an Illumina HiSeq X-Ten platform. Raw reads were firstly separated by cell barcodes, then trimmed, and aligned to the mm9 mouse transcriptome and de-duplicated by UMIs information as described previously.
GEO Accession
GSM4279844