GSM4677565: h3k27me3_emf1_2; Arabidopsis thaliana; ChIP-Seq
Chromatin was extracted from 10-day-old whole seedlings. Anti-H2Aub (Cell Signaling Technology, 8240S) and anti-H3K27me3 (Millipore, 07-449) antibodies were used for chromatin immunoprecipitation. Libraries were prepared with end repair, A-tailing and ligation of Illumina-compatible adapters using the Ovation® Ultralow Library Systems (NuGEN). The ligated product was amplified with 14 cycles of PCR. DNA of a size range between 200 and 600 bp was purified from an agarose gel. Amplification was confirmed by testing an aliquot of the library before and after amplification by qPCR.
GEO Accession
GSM4677565