GSM1961088: ba_d2_k4m1; Mus musculus; ChIP-Seq
Cells were crosslinked with 1% formaldehyde for 10 min at 37◦C; then, crude nuclei were purified. Chromatin was fragmented by sonication with a Bioruptor UCD-300 (Diagenode) to obtain fragments 200–800 bp in length. For each ChIP assay, 2–5 ug of antibodi_x0000__x001A__x001B__x0003__x0000__x0019__x001B__x0005__x0000__x001A__x0006__x0005_￸_x000B__x0000__x0000__x0000__x0000__x001B__x0003__x0000__x0017__x001B__x0005__x0000__x0013__x001B__x0005__x0000__x001A_'_x0003__x0000__x001A__x001B__x0003__x0000__x0019__x0000__x0000__x0000__x0000__x001B__x0005__x0000__x001A__x001B__x0005__x0000__x001A__x001B__x0003__x0000__x0017_'_x0005__x0000_  _x001B__x0003__x0000__x0019_%_x0005__x0000_  _x0000__x0000__x0000__x0000__x001B__x0000__x0000_&_x001B__x0003__x0000__x0019__x001B__x0005__x0000__x0013__x001B__x0003__x0000__x0019__x0000__x0000__x0000__x0000__x001B__x0005_￶_x001C__x001B__x0005__x0000__x001A__x001B__x0005__x0000__x0013_%_x0005__x0000_  '_x0002__x0000__x0015_%_x0005__x0000_  _x001B__x0003__x0000__x0019_'_x0003__x0000__x001A__x0006__x0005__x0000__x000B__x0000__x0000__x0000__x0000_%_x0003_ ChIP-seq libraries were generated following the procedue described in "TELP, a sensitive and versatile library construction method for next-generation sequencing" by Peng X, Wu J, Brunmeir R, Kim SY, Zhang Q, Ding C, Han W, Xie W, Xu F., published in Nucleic Acids Res. 2015 Mar 31;43(6):e35. doi: 10.1093/nar/gku818. Epub 2014 Sep 15.
GEO Accession
GSM1961088