miRNA-seq of Human T cell IL2
Total RNA from human CD4 (+) cells which were cultured in the presence of IL2 was extracted with chloroform and phenol. The miRNA-seq libraries were prepared using the NEBNext Multiplex Small RNA Library Prep for Illumina protocol (New England BioLabs, Ipswich, MA, USA) and sequenced on the Hiseq 2500 using v4 chemistry for single end sequencing (San Diego, CA, USA).