GSM1630522: Mel202 DD-wt-SF3B1 plus Shld (2 days); Homo sapiens; RNA-Seq
The gel-free protocol was employed for the TruSeq RNA Sample Prep Kit per manufacturer’s specifications, and performed on the Beckman Coulter Biomek FXp robotics platform. The standard RNA fragmentation profile was used as recommended by Illumina (94 degrees Celsius for 8 minutes). The PCR-amplified RNA-seq library products were then quantified using the Advanced Analytical Fragment Analyzer Standard Sensitivity NGS Fragment Analysis Kit (catalog number DNF-479). The samples were diluted to 10 nanomolar in Qiagen Elution Buffer (Qiagen material number 1014609), denatured, and loaded at 2.75 picomolar on an Illumina HiSeq2500 in Rapid Run mode using TruSeq Rapid PE Cluster Kit  – HS (catalog number PE-402-4001) and TruSeq Rapid SBS Kit – HS (200 cycle) reagents (catalog number FC-402-4001). The RNA-seq libraries were sequenced at 100 base-pair paired-end with a 7-base-pair index using the standard Illumina primers. The sequence intensity files were generated on the instrument using the Illumina Real Time Analysis software. The intensity files were demultiplexed and fastq files created using the CASAVA 1.8.2 software suite.
GEO Accession
GSM1630522