GSM4227629: LN2.1; Mus musculus; RNA-Seq
RNA extraction and purification from tissue snap frozen in liquid nitrogen was done via homogenization in Trizol and the Qiagen miRNeasy Mini Kit (skeletal muscle). Five microliters of 2 nM pooled libraries per lane will then be denatured, neutralized and applied to the cBot for flow cell deposition and cluster amplification, before loading to HiSeq 4000 for 75b paired-end sequencing (Illumina, Inc.). Approximately 30M reads per library is generated
GEO Accession
GSM4227629