GSM4565569: Untreated_OVCAR8_Day9; Homo sapiens; OTHER
Genomic DNA was isolated using a QiAmp DNA blood extraction kit. Libraries were prepared as in: Wang T, Lander ES, & Sabatini DM (2016) Viral Packaging and Cell Culture for CRISPR-based Screens. Cold Spring Harb Protoc 2016(3): pdb.prot090811 PMID: 26933250, with the following exceptions: Forward PCR primer: AATGATACGGCGACCACCGAGATCTACACGAATACTGCCATTTGTCTCAAGATCTA  DNA Polymerase: ExTaq (Takara)  Genomic DNA/50 μL PCR reaction: 6 μg  Amplification cycles: 28
GEO Accession
GSM4565569