GSM2837065: KHYG-1; Homo sapiens; RNA-Seq
RNA was extracted using miRNeasy Mini Kit (Qiagen). Agilent Bioanalyzer RNApico chip (Agilent) was used to evaluate the integrity of RNA and Qubit RNA kit (Life Technologies) to quantitate RNA in samples. 1.5 µg of total RNA was used for ScriptSeq v2™ Complete kit for human/mouse/rat (Illumina) to ribodeplete rRNA and further for RNA-seq library preparation. SPRI beads (Agencourt AMPure XP, Beckman Coulter) were used for purification of RNAseq libraries. The library QC was evaluated on High Sensitivity chips by Agilent Bioanalyzer (Agilent). Sequencing libraries were sequenced using paired end 100 bp read format on an Illumina HiSeq 2000 instrument (Illumina). Ribo depletion RNA-Seq: Agilent Bioanalyzer RNApico chip (Agilent) was used to evaluate the integrity of RNA and Qubit RNA kit (Life Technologies) to quantitate RNA in samples. 1.5 µg of total RNA was used for ScriptSeq v2™ Complete kit for human/mouse/rat (Illumina) to ribodeplete rRNA and further for RNA-seq library preparation. SPRI beads (Agencourt AMPure XP, Beckman Coulter) were used for purification of RNAseq libraries. The library QC was evaluated on High Sensitivity chips by Agilent Bioanalyzer (Agilent). Sequencing libraries were sequenced using paired end 100 bp read format on an Illumina HiSeq 2000 instrument (Illumina).
GEO Accession
GSM2837065