Whole embryo Hi-C
3cm (nose to tail) d/d embryos were kept under starving conditions for 3 days to avoid genomic DNA contamination from food. After starving, intestines and guts were removed, the remaining parts were dissected from 10 embryos and washed with 80% diluted PBS (APBS) under the anesthetic conditions. The dissected pieces were treated with Liberase TM (1:100 dilution Unit) for 30 min with rotation. Cells were spun down, resuspended into 10%FCS/AMEM. 1x 106 cells were resuspended into 36ml of 10%FCS/AMEM. Restriction enzyme for the Hi-C library prep was DpnII