Escherichia coli KLF2604 (pLEPA) (complemented) replicate 3 ribosome footprints
Lysates of mid-log phase cells were treated with micrococcal nuclease in order to digest the polysomes. RNA was extracted from the resulting monosomes and the ribosome protected footprints were gel purified. These footprint fragments were converted into cDNA libraries and deep sequenced.