Nalm6_Iso-Seq
A 4.5-10kb size fraction was eluted, re-amplified and cleaned with AMPure PB beads. A SMRTBell library was constructed using 2.3ug of size-selected cDNA. A total of 2 SMRT Cells (one 6 hour and one 10 hour movie) were sequenced on the PacBio Sequel platform using 2.0 chemistry.