sRNA-seq of Arabidopsis lyrata leaves
Total RNA was isolated from leaves of A. lyrata MN47 using the Qiagen miRNeasy Mini Kit (catalog #217004). A total of 3g of RNA was sent to LC sciences (Houston, TX, USA) were an Illumina TruSeq Small RNA library was constructed and sequenced, leading to the obtention of approximately 14 million 150 bp reads.