GSM3557548: rna_TIF1_a; Saccharomyces cerevisiae; OTHER
Cells were rapidly vacuum filtered and flash frozen. Whole cell extracts were prepared by breaking cells in a freezer mill.  SSU RNaseI footprints and total RNA fractions were extracted from each yeast culture essentially as described in Archer SK et al (2016),Nature 535, 570-574. Sequencing libraries were generated (size selection, preadenylated linker ligation, reverse transcription, cDNA circularization, PCR amplification) as described in Ingolia NT et al 2009.  Science 324(5924):218-223
GEO Accession
GSM3557548