GSM2677362: 06 SH-SY5Y; Homo sapiens; RNA-Seq
Total RNA was extracted using Illumina´s TruSeq RNA Sample Preparation Kit. Barcoded mRNA-seq cDNA libraries were prepared from 600 ng of total RNA. mRNA was isolated using Oligo(d)T magnetic beads. Isolated mRNA was fragmented using divalent cations and heat and converted into cDNA using random primers and SuperScriptII (Invitrogen), followed by second strand synthesis. cDNA was end repaired, 3’ adenylated and single T-overhang Illumina multiplex specific adapters were ligated to the cDNA fragments, followed by an enrichment PCR. All cleanups were done using Agencourt AMPure XP magnetic beads.
GEO Accession
GSM2677362