GSM2338067: small RNA-seq_ade6-INT wt; Schizosaccharomyces pombe; miRNA-Seq
Total RNA was isolated from exponentially growing cells using hot phenol. The RNA was fractionated using RNeasy Midi columns (Qiagen). The flow-through fraction was precipitated and aliquots of 25 ug were separated by 17.5% PAGE to isolate the 18–28-nucleotide fraction. Libraries were prepared using the Illumina TruSeq small RNA kit according to manufacturers instructions. For barcodes, see sample characteristics.
GEO Accession
GSM2338067