The transcriptome of Polyrhabdina sp. WS-2016
Total RNA was extracted by RNAqueous Micro Kit according to manufacturers instructions but without the DNase I digest step, then reverse-transcribed and amplified by using SMART-Seq v4 Ultra Low Input RNA Kit (20-30 amplification cycles; optimization was done as in SMARTer Pico PCR cDNA Synthesis Kit). Indexed TruSeq libraries were sequenced as paired-end 150 bp reads in two multiplexed lanes on the Illumina HiSeq 4000 machine (i.e., two runs were done of each sample).