Transcriptomic Analysis of the Woodchuck Model of Chronic Hepatitis B
Total RNA from the pooled samples was purified to mRNA to reduce ribosomal RNA (rRNA) amounts using the PureBiotech MRRK1010 mRNA Purification Kit (PureBiotech LLC, Middlesex, NJ). 200 ng of mRNA was converted to Roche 454 Sequencing ready cDNA following the cDNA Rapid Library Preparation Method from Roche (USM 00064 GS FLX Titanium Series, cDNA Rapid Library Preparation Method Manual, Roche Applied Sciences, Indianapolis, IN). The cDNA libraries were sequenced using the Roche 454 Genome Sequencer FLX using Titanium Chemistry following the manufacturer’s recommendations (GS FLX Titanium Series Sequencing Method Manual, Roche Applied Sciences, Indianapolis, IN).