GSM2334959: tbx5_2neg; Danio rerio; RNA-Seq
RNA was extracted using Arcturus Pico Pure (Thermofisher) following manufacturer instructions. 0.6 ng of total RNA was used to generate barcoded RNA-seq libraries using the Ovation Single Cell RNA-Seq System (NuGEN) with two rounds of library amplification.  The size of the libraries was calculated using the Agilent 2100 Bioanalyzer. Library concentration was determined using the Qubit® fluorometer (ThermoFisher Scientific). Libraries were sequenced on a HiSeq2500 (Illumina) to generate 60 bases single reads.  4 biological replicates consisting of 5 pooled hearts were used per sample.
GEO Accession
GSM2334959