GSM1528644: STT5858_run_1; Homo sapiens; RNA-Seq
Multiple 2mm-diameter cores were taken from diagnostic FFPE material for RNA isolation. Then, total RNA was extracted by using of RecoverAll Total Nucleic Acid Isolation Kit (Ambion). After ribo-depletion, RNA-SEQ libraries were constructed with a modified TruSeq Sample Preparation Kit using random primer (TruSeq™ RNA Sample Prep Kit v2 -Set A (48rxn) , RS-122-2001, Illumina, San Diego, CA, USA). Then the libraries were sent to Stanford Center for Genomics and Personalized Medicine to be sequenced on an Illumina HiSeq2000 platform with a paired-end 2X101-base protocol.
GEO Accession
GSM1528644