FAIRE-seq of the human megakaryocytic cell line CHRF-288-11
FAIRE-seq of the human megakaryocytic cell line CHRF-288-11
CHRF 288-11 cells were cultured in RPMI-1640 (Sigma-Aldrich, Gillingham, UK, R0883), 10 % FBS (Invitrogen, Paisley, UK, 10100-147) and 200 uM L-glutamine, 100 U penicillin and 100 ug/ml streptomycin (Sigma-Aldrich, G1146). We performed FAIRE in the human megakaryocytic cell line CHRF-288-11. Cells were maintained in RPMI-1640 medium (Sigma-Aldrich, R0883), 20 % horse serum (heat- inactivated, Invitrogen, 26050-088), and 1 % L-glutamine-penicillin-streptomycin solution (Sigma-Aldrich, G1146), and grown at 37C, 5 % CO2, and 100% humidified atmosphere. FAIRE experiments were carried out as previously described. Twenty million cells were cross-linked with 1 % formaldehyde for 12 min and subjected to 12 sonication cycles (30 sec of high pulse, 30 sec of rest) using the Bioruptor UCD-200. A DNA amplification step was neglected. The sample was cleaned-up using the QIAGEN MinElute PCR Purification Kit (Qiagen, 28004). FAIRE DNA was processed following the Illumina paired-end library generation protocol.
Experimental Factor: CELL_LINE
CHRF-288-11