GSM1631164: Myla_Azacitidine; Homo sapiens; Bisulfite-Seq
DNA was isolated using TRIzol, according to manufacturer’s instructions Total of 3ug of DNA was used as input amount in the Agilent SureSelectXTMethyl-Seq Target Enrichment System for Illumina Multiplexed Sequencing protocol. Quality of the isolated DNA was assessed by an Agilent 2200 Tapestation. DNA was sheared using a S220 Focused-ultrasonicator (Covaris). Bisulfite conversion was done using EZ DNA Methylation Kit from Zymo Research according to manufacturer’s instructions.
GEO Accession
GSM1631164