GSM2071830: C89; single cell; Mus musculus; RNA-Seq
Embryos at e13.5 were dissected in cold PBS, the pancreas was isolated and then disociated into single cells using trypsin. Single cells suspension at a concentration of 200cell/ul was loaded on a microfluidic device. cDNA was prepared from each cell using the SMARTer Kit (Takara-Clontech, CA) Single cell RNA libraries were prepared for sequencing using the low throughput Nextera XT SNA library prep kit (Illumina, CA), according to the manufacturers protocol
GEO Accession
GSM2071830