GSM4504167: OE2-IP; Homo sapiens; RIP-Seq
Total RNA was extracted with TRIzol reagent. mRNA was enriched from total RNA with Poly(A) mRNA Magnetic Isolation Module(NEB#E7490) before fragmented chemically to ~150 nts long size, 10% fragmented mRNA was saved as Input sample while the rest part was subjected to IP using anti-m6A antibodies(Synaptic system#202 003). After stringent washing, bound RNA was eluted by competition with N6-methyladenosine (Sigma-Aldrich#M2780) as IP samples. IP and input library construction was carried out simultaneously with VAHTS Total RNA-seq (H/M/R) Library Prep Kit for Illumina (Box2&3, Vazyme#NR603). Qualities of each library were validated using an Agilent 2100 Bioanalyzer, and successful libraries of sample pairs (input and IP) were sequenced on illumina Novaseq sequencer with PE150 strategy. MeRIP-seq
GEO Accession
GSM4504167