GSM2577605: ChIP-seq-Mammary-WT-L1-H3K27me3-rep1; Mus musculus; ChIP-Seq
All tissues were snap frozen and stored at -80°C. Tissues were pulverized in liquid nitrogen followed by chromatin fixation with 1% formaldehyde final concentration. Washed chromatin (PBS) was homogenized using a dounce tissue grinder in Farnham's lysis buffer and followed by chromatin fragmentation through sonication. Fragmentation efficiency in the range of 200 bp - 500 bp was controlled by gel electrophoresis and ChIP was performed using Dynabeads Protein A (Invitrogen) and the following antibodies: CTCF (Abcam ab70303 / Millipore 07-729) and H3K27ac (Abcam ab4729). Indexed libraries were produced using the NEBNext Ultra II DNA Library Prep Kit and NEBNext Index Primer Set for Illumina according to the manufacturer's protocol. Fragments between 200 bp and 500 bp were selected by gel electrophoresis and 50 bp single-end sequencing was performed on an Illumina HiSeq 2500. Libraries for sequencing were prepared using standard Illumina protocols.
GEO Accession
GSM2577605