RNA-seq of P. lunatus domesticated accession G27455. Leave tissue
RNA samples were quantified with Nanodrop 2000 and quality was assessed with Agilent Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA) and agarose gel electrophoresis. Samples showing a 260/280 ratio of absorbance between 1.9 - 2.0 and RIN (RNA Integrity Number) values above seven were selected. mRNA samples were enriched using oligo(dT) beads and randomly fragmented, then cDNA was synthesized using mRNA templates. RNA-seq library preparation was completed through size selection and PCR enrichment. Sequencing of the library was accomplished using a paired-end 150 run (2150 bases) on an Illumina HiSeq Platform (Illumina, San Diego, CA , USA).