low input RNA-seq of homo sapiens: adult peripheral MAIT cells: Sample MAIT_IGTB187_72
We sorted 7 lymphocyte populations for 6 healthy donors, in duplicate, with 1000 cells per sample. These samples were randomized in a 96 well plate and we performed Smart-seq2 library preparation, where RNA is polyA selected. Libraries were then paired-end sequenced with each read being 25bp long.