GSM2754267: Morc2bWT_4; Mus musculus; RNA-Seq
RNA extraction by TRIzol reagents Equal amounts (1 mg) of total RNA from each sample were used to generate RNA-seq libraries using TruSeq® Stranded mRNA Library Preparation Kit Set A (Cat. No. RS-122-2101, Illumina) according to the manufacturer’s instruction. The concentration of DNA library templates was determined using a Qubit 3.0 Fluorometer (Thermo Fisher Scientific). The quality of libraries was evaluated using the Agilent 4200 TapeStation (Agilent Technologies). Eight individual libraries (4 wild type and 4 Morc2b-/-) were pooled in equal amounts for sequencing using the Illumina NextSeq 500/550 High Output v2 kit (Illumina, 75 cycles, FC-404-2005) and the NextSeq 500 system (Illumina).
GEO Accession
GSM2754267