16S rRNA amplicon library: Peat porewater
Porewater for 16S rRNA gene sequencing was collected from piezometers in June 2016 and filtered onto polycarbonate membrane filters (47 mm, 0.2 m pore size, Whatman) within 1 hour of sampling. The samples were frozen at -20C immediately, and then shipped on dry ice to Georgia Institute of Technology, where they were stored at -80C until further processing. DNA was extracted from whole filters using the DNeasy PowerSoil extraction kit (Qiagen). Amplification and sequencing of the 16S rRNA gene was performed using the universal primer pair 515F and 806R.