Multiplexed Bar-Seq Reads from Yeast Knockout Collection
Libraries for next-generation sequencing were prepared by PCR-amplification of genomic DNA (LightCycler 480 Instrument II, Roche), purification (Zymo Research). Primers used for indexing (culture, timepoint) and sequencing adapters were added via PCR. NextSeq sequencing (Harvard Biopolymers Facility) was used to sequence the culture index, the timepoint index, and a 55 bp single end read of the barcode construct. PhiX was added at 50% to increase sequence diversity.