GSM3618291: Viable_paternal_excess_1 [Bisulfite-seq]; Arabidopsis thaliana; Bisulfite-Seq
Endosperm was hand dissected from seeds at 7 DAP. DNA was extracted from dissected endosperm using the QiaAMP DNA microkit. Breifly, dissected tissue was incubated in ATL buffer and Proteinase K overnight. 80-100 ng of DNA obtained for each sample was subjected to bisulfite treatment using Methylcode Bisulfite conversion kit (Invitrogen). Purified, bisulfite converted DNA was used as input for library construction using Illumina Truseq DNA methylation kit.
GEO Accession
GSM3618291