GSM2837084: JAR CK-3; Homo sapiens; RNA-Seq
Total RNA was extracted with the RNeasy Mini Kit (Qiagen, Hilden, Germany).RNA integrity was analyzed by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US). Qualified total RNA was further purified by RNAClean XP Kit (Beckman Coulter, Kraemer Boulevard Brea, CA, USA) and RNase-Free DNase Set (Qiagen). Libraries were prepared according to the Whole Transcriptome Sequencing Protocol (ShanghaiBio Corporation, Shanghai, China). Steps briefly included the followings: mRNA purification with RNA Purification Beads, mRNA fragmentation, synthesis of the first strand cDNA, synthesis of the second strand cDNA, end repair, 3' end adenylation, adapters ligation, PCR amplification,library quality control.Libraries were sequenced on the HiSeq X Ten (Illumina, San Diego, CA, USA) following the manufacturer's protocols.
GEO Accession
GSM2837084