DRS, ESRP overexpression (MDA-MB-231 celline)
RNA oligoribonucleotide templates were ordered from IDT. The sequences were Oligo 1, 5'-AGAGUCCCAUCCUCACCAUCAUCACACUGGAAGACUGCAG-3'; Oligo 2, 5'-CUGGUGCAGCACUCUCGACGGCACCUAUCUGCCAUCGUAG-3'; Oligo 3, 5'-CGAUCGUCACUAUCUGCAUCAGUAGCUCUAGCAUACUGAG-3'; Oligo 4, 5'-UCUUUCGUCAGGGCAGAGGAUGGAUGCAAGGAUAAGUGGA-3'. Polyadenylation was performed by using a poly#A# tailing kit #Ambion#. 3'deoxyATP # cordycepin triphosphate, Jena Biosciences# was introduced 10min after the initiation of the polyadenylation reaction for 3'-end blocking and tail length limitation. Reaction products were cleaned by phenol-chloroform extraction and ethanol precipitation. Samples were analysed with microcapillary electrophoresis #Agilent Technologies#