ddRAD of Conus sanguinolentus and C. lividus
We utilized approximately 200 ng of DNA for ddRAD library preparation following established protocols. We digested genomic DNA samples with restriction enzymes EcoRI-HF and MseI at recommended conditions. We targeted 294-394 basepair (bp) fragments from the digested DNA with a Pippen Prep device  following recommended protocols. We then submitted size-selected libraries to the University of Michigan DNA Sequencing Core facilities (now known as the Advanced Genomics Core). The samples were indexed and run together on a single lane with other samples on the Illumina HiSeq 4000 platform with 150 bp paired-end sequencing.