GSM1961198: Total RNA pol II ChIP-seq S194 5'SP R2; Mus musculus; ChIP-Seq
cross-linked Chromatin was sonicated with a Bioruptor IPs from 1-2 mg of cross-linked extract were immunoprecipitated, and prepared for illumina library construction by repair with Klenow, T4 DNA polymerase and T4 polynucleotide kinase, A-tailing with Klenow exo-, ligation to branched adaptors, gel purification of ~200bp fragments, and amplification (18cycles) with Illumina Tru-seq primers using Phusion DNA polymerase. ChIP-seq 1X 50 base reads
GEO Accession
GSM1961198