GSM2417261: LW_Liver_90d_miRNA-seq; Sus scrofa; miRNA-Seq
Total RNA was extracted from the three liver samples using TRIzol (Invitrogen). RNase-free DNase I (TaKaRa) was used to remove genomic DNA from the RNA samples. The integrity of total RNA was determined by the Agilent Technologies of Bioanalyzer 2100 and RNA 6000 Nano Labchip Kit (Agilent, CA, USA) with RIN number >6.0. According to the Illumina EAS Small RNA Sample Prep Protocol. In general, approximately 15 ug of small RNA-enriched total RNA was prepared for High-throughput sequencing. In brief, High-throughput sequencing was performed as following successive step: For each library, the small RNA ranged from 14 to 40 nt were purified by polyacrylamide gel electrophoresis (PAGE) and ligated with proprietary adaptors (Illumina). Then the modified small RNA was reverse transcribed and amplified by RT-PCR. Finally, the enriched cDNA were sequenced on the Illumina Hiseq 2500/2000 platform and 50 bp single-end reads were generated.
GEO Accession
GSM2417261