tsICP4 HSV-1 Productive Infection
MRC5 cells were infected with temperature sensitive ICP4 HSV-1 (tsKos) at  an MOI of 10 PFU/cell for four or six hours. Samples were grown at permissive  (34), nonpermissive (39.6) or shifted up at 4 hpi from permissive to  nonpermissive. ChIP-Seq for Pol II was performed. Illumina libraries were  constructed for the IP and input material. Illumina sequencing was carried  out at Tufts University Core Facility using HiSeq 2500 to generate 50 bp  single-end reads.