iPSC-RPE RNA: Sample 23ee507d-32aa-4ae4-b426-8689ad77b7de
Total RNA was isolated using the Quick-RNA Mini Prep Kit (Zymo) from frozen RTL plus pellets, including on-column DNAse treatment step. RNA was eluted in 40 µl RNAse-free water and run on a Bioanalyzer (Agilent) to determine integrity. Concentration was measured by Qubit. Illumina Truseq Stranded mRNA libraries were prepared and sequenced on HiSeq4000, to an average of 40 M 150 bp paired-end reads per sample. 
alignment_software
STAR