Soybean leaf fed with xylem sap from non-nodulating plants
mRNA was isolated from total RNA using Sera-Mag Oligo-dT beads and fragmented with divalent cations. Fragmented mRNA was reverse transcribed to cDNA using random primers and Superscript III (Invitrogen). End repair was performed to blunt end cDNA followed by ligation of 3’ adenine base overhangs. Oligonucleotide adapters with thymine overhangs were ligated to the cDNA. Ligated fragments of approximately 200 bp were selected and purified from an agarose gel. PCR enrichment of the libraries was performed before each library was sequenced with 75 bp single end reads on one lane of the Illumina GAIIx