RNA seq of grapevine : virus-symptomed leaves
Frozen leaf powder were transported to Huada Gene Co., Ltd., Shenzhen, China on dry ice for small RNA extraction, library construction and deep sequencing (BGISEQ-500 sequencing platform). The clean small RNA reads were obtained by removing low-quality, 5 ' and 3' adapters-linked , polyA-contained and less than 18nts tags. The quality of sequencing was evaluated through Q20 (the ratio of the number of nucleotides with a quality value greater than 20 to the total number of nucleotides in the sample) and the small RNA types were analyzed.