GSM2149895: riboseq_shoot_bufferB; Arabidopsis thaliana; OTHER
About 0.1 g of pulvernized root or shoot tissue was resuspended in 600 or 800 µl of polysome extraction buffer. The lysate was treated with RNase to obtain ribosome footprints. Ribosomes were isolated with size exclusion columns (illustra MicroSpin S-400 HR Columns, GE Healthcare, Cat. # 27-5140-01). Purified ribosome footprints were then subjected to rRNA depletion. Libraries were constructed according to ARTseq/TruSeq Ribo Profile Library Preparation Kit manual
GEO Accession
GSM2149895