GSM3391601: 7-day ADX rep2; Rattus norvegicus; RNA-Seq
Immediately after decapitation, hypothalamic tissue containing the PVH was dissected out on ice, frozen on dry ice, and RNA was harvested using TrizolTM followed by purification with RNeasy mini kit TM reagents and column DNase digestion. Illumina TruSeq RNA Sample Prep Kits, version 2, was used with 1 microgram of total RNA for the construction of sequencing libraries. mRNA libraries were constructed from 1,000 ng high-quality total RNA using the Illumina TruSeq RNA Sample Prep Kits, version 2. The resulting cDNA was fragmented using a Covaris E210 (Covaris, Inc., Woburn, MA). Library amplification was performed using 10 cycles to minimize the risk of over-amplification.
GEO Accession
GSM3391601