Bisulfite-Seq of maize: mop1 mutant immature ear (4-6 cm) replicate 2
The immature ears (4-6 cm) of the F1 individuals B73/Mo17;mop1/mop1 were collected in the greenhouse, flash-frozen in liquid nitrogen and preserved at -80. DNA was extracted from frozen tissue using the modified CTAB method, and high-throughput 150 bp paired end whole genome bisulfite sequencing was performed.