GSM2148039: L51Lib48; Saccharomyces cerevisiae; RNA-Seq
The RNA Mini Kit (Qiagen, Madrid) was used following manufacturer’s instructions. RNA quality was checked using the Bioanalyser (Agilent Technologies Spain, Madrid) and samples with good quality RNA, showing 5 as minimum of RNA Integrity Number (RIN) were sequenced Illumina RNA amplification kit (Thermo-Fisher) was used in order to amplify the low amount of RNA extracted from cells separated by cytometry. Sequencing was performed with the 5500 SOLiD system (Life Technologies, Madrid). Libraries were performed using standard SOLID protocol.
GEO Accession
GSM2148039