Illumina HiSeq 2500 sequencing; Selaginella expression atlas
Selaginella expression atlas
The sampled organs were flash-frozen in liquid nitrogen and ground with mortar and pestle. The plants used for the tissue-expression atlas and temperature stresses were grown at 24°C under 16 hour light (90 μmol photons m-2 s-1) and 8 hour darkness photoperiod on soil, while the plants used for high light and darkness stresses were grown on modified Hoagland solution (0.063 mM FeEDTA; 0.5 mM KH2PO4; 2.5 mM KNO3; 2 mM Ca(NO3)2 × 4H2O; 1 mM MgSO4 × 7H2O; 50.14 µM H3BO3; 9.25 µM MnCl2 × 4H2O; 1 µM ZnCl2; 1 µM CuCl2; 0.5 µM Na2MoO4 × 2H2O, pH 5.8, 0.8% agar) at 24°C under 12 hour light (70 μmol photons m-2 s-1) and 12 hour darkness before being subjected to the prolonged darkness (48 hours) and high light (150 μmol photons m-2 s-1) treatments.  The total RNA was extracted using Plant RNeasy Plant kit (Qiagen) according to the manufacturer's instructions. The libraries were prepared from total RNA using polyA enrichment. The cDNA libraries were constructed using RS-122-2001/RS-122-2002,  TruSeq RNA Library Prep Kit v2 according to the manufacturer's instructions.
Experimental Factor: organism part
vascular leaf
Experimental Factor: sampling site
not applicable
Experimental Factor: temperature
24
Experimental Factor: stimulus
none
Experimental Factor: time
noon