GSM3692402: HEK293_dsGFPc_PACT knock-out_APP_Rep2_Experiment1; Homo sapiens; miRNA-Seq
Ago-pulldown: Ago Affinity Purification by Peptides was performed in accordance with an earlier described protocols (Hauptmann et al., 2015). Ago-pulldown quality was validated by miR16/21 RT-qPCR. RNA extraction: 1.5-3*10^6 cells were trypsinized and washed twice with PBS before proceeding with RNA extraction. RNA was extarcted using TRI reagent (Sigma Aaldrich) according to manufacturer's recmmendations. Total RNA integrity and quality was validated on the Nanodrop 2000 (Thermo Scientific) and on an 1% agarose gel. Libraries were prepared with total RNA and Ago-purified RNA, from approximately 2-3*10^6 cells, with the Lexogen small RNA-Seq Library Prep kit (Lexogen) according to the manufacturer's instructions.
GEO Accession
GSM3692402