GSM3169135: PH5CH8 control  IFN Lambda treated at 24hr rep 2; Homo sapiens; RNA-Seq
Total RNA was extracted using RNeasy mini kit (QIAGEN) with on-column DNase treatment. KAPA stranded RNA-seq kit with Ribo Erase Library quality was evaluated using the Qubit® 3.0 Fluorometer and the Agilent 2100 Bioanalyzer instrument. Constructed libraries were sequenced on an NextSeq 500 Illumina platform, producing 2x75nt stranded paired end reads (52 Gb). Image analysis, base calling, and error estimation were performed using Illumina Analysis Pipeline (version 2.8).
GEO Accession
GSM3169135