molecular inversion probe sequencing of mus musculus: lung metastasis from mouse mTSG-N8R injected with TBG-mTSG, treated with anti-CTLA4
MIPs were designed according to previously published protocols. Briefly, the 70 bp flanking the predicted cut site of each sgRNA of all 278 unique sgRNA were chosen as targeting regions, and the bed file with these coordinates was used as an input. Since Trp53 sg4 targets a similar region as the p53 sgRNA within the base vector, the same MIP was used to sequence both of these loci.These coordinates contained overlapping regions which were subsequently merged into 173 unique regions.