GSM4227628: Genomic Input-ChipSeq BK3-4 input; Mus musculus; ChIP-Seq
For H3K27me3 and H3K36me3 ChIP-Seq, 40 million of cells were cross-linked with 1% formaldehyde for 10 minutes, followed by addition of glycine to stop crosslinking. After washing, cell lysis and sonication, the chromatin samples were incubated with antibody-conjugated Dynabeads (Invitrogen) at 4 degrees Celsius. Beads bound with chromatin were then subject to extensive washing and elution. Eluted chromatin was de-crosslinked overnight at 65 degrees, followed by protein digestion with proteinase K and DNA purification with Qiagen PCR purification kit. The obtained ChIP DNA samples were submitted to the UNC-Chapel Hill High-Throughput Sequencing Facility (HTSF) for preparation of multiplexed libraries and deep sequencing with an Illumina High-Seq platform according to the manufacturer's instructions.
GEO Accession
GSM4227628