GSM2756552: NeuHC104; Homo sapiens; RNA-Seq
Neutrophils from whole blood leukocytes were sorted based on positive expression of CD16 and negative expression of CD14 (monocytes) and CD41 (platelets attached to neutrophils); sorting was performed using FACSAriaIII (BD). Neutrophils were lysed in Trizol LS (Thermo Fisher) directly after sorting. Sequencing libraries were prepared using the Rapid Directional RNA-Seq Kit (NEXTflex)
GEO Accession
GSM2756552