P.astreoides SML bacteria
The average size of the amplicon was 528 bp measured using a Bioanalyzer 2100. The amplicon pool was purified following the Roche protocol: Pool was Ampure purified three times then diluted to 10^6 copies of DNA/ul. This diluted library was then used as the template for 32 individual Library-A emulsion PCRs. Following amplification of the templates, the emulsions were broken using isopropanol then recovered on a 0.45 um filter. Beads from the emulsion that were DNA positive were selected via biotin binding and the negative DNA beads removed. For this run, we used two copies of DNA per bead (on average) which produced 13.4% positive (ideally this is between 10-15 and no higher than 20 FYI). DNA positive beads were counted on a Beckman Coulter counter and then loaded on an 8 region Ti picotiter plate. Your samples were loaded in regions 3 and 4.