GSM3097743: SI-10 (A_1); Sus scrofa; miRNA-Seq
Spleens were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Three individuals' RNA samples, of the same group at each time point were equally mixed to generate an RNA pool. A total amount of 3 μg total RNA per pool was used as input material for the small RNA library. Sequencing libraries were generated using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB, USA.).
GEO Accession
GSM3097743