Genomic DNA of phage OY1
When constructing the DNA library to be tested, sequencing primer binding sites are added to the adapters at both ends. After the first round of sequencing is completed, the template chain of the first round of sequencing is removed, and the paired-read module is used to guide the complement The strand is regenerated and amplified in place to achieve the amount of template used in the second round of sequencing, and the second round of synthetic sequencing of the complementary strand is performed