RNA-seq of Leptinotarsa decemlineata: larval midgut PVY-reared
Total RNA was isolated using Trizol,  rRNA was depleted using RiboMinus kit and Dnase I treated. RNAseq library was prepared according to Applied Biosystems protocol: fragmentation by RNAse III (AB), adaptor hybridisation and ligation, reverse transcription using ArrayScript RT (AB), cDNA purfication using QIAGEN PCR Purification Kit, size-selection on 6% TBE-Urea gel (150-250 nt excised), cDNA amplification by PCR (15 cycles) and purification (PureLink Micro Kit), 6 full scale ePCR reaction (AB ePCR kit).