GSM1531288: SD rep 3; Tigriopus californicus; RNA-Seq
Total RNA was extracted using standard Tri-reagent protocol and then purified in QIAGEN RNeasy columns. The Illumina mRNA sequencing kit (RS-100-0801) and multiplexing kit (PE-400-1001) were used to isolate mRNA and prepare sequencing libraries. Matching barcodes were assigned to the SD control and heat stress sample (TGACCA), as well as the SC control and heat stress samples (GCCAAT), to allow for multiplexing on two lanes of an Illumina GA-II genome analyzer flow cellibrary construction protocol Groups of samples were sequenced on different runs but always on the same lane, with samples barcoded as needed.   Libraries were grouped and sequenced as follows: run 1: SD rep 1 + SCN rep 1 run 2: SD rep 2 run 3: SD rep 3 + SCN rep 2 run 4: SDf x SCNm rep 1 run 5: SDf x SCNm rep 2 + SCNf x SDm rep1 + SCNf x SDm rep2
GEO Accession
GSM1531288