np_UCD646So_gDNA_illumina
DNA libraries were prepared as described in Jones et al. (2014). DNA was fragmented with a Bioruptor NGS (Diagenode). Libraries were prepared using the KAPA KK8201 library preparation kit (KAPA Biosystems) using 1 ľg of DNA as input and NEXTflex primers as barcodes (BIOO Scientific). Size selection was carried out using the E-Gel system (Life Technologies). Paired-end reads of 150 bp in length were sequenced on an Illumina HiSeq2500 machine at the DNA Technologies Core at UC Davis.