RNA-seq of HeLa cells: H2A.Z.2 siRNA
The mRNA fraction was selceted from the total RNA provided before conversion to cDNA. Second strand cDNA synthesis incorporated dUTP. The cDNA was end-repaired, A-tailed and adapter-ligated. Prior to amplification, samples underwent uridine digestion. the prepared libraries were size selectd, multiplexed and QC'ed before paired en sequencing over one lane of a flow cell.