np_UCD646So_gDNA_pacbio
Around 15 micrograms of high quality genomic DNA were sheared using a Megaruptor (Diagenode). After shearing, fragmented DNA was end-repaired and ligated to universal hairpin adapters to prepare SMRTbell template using SMRTbell Template Prep Kit 1.0 (Pacific Biosciences) following the manufacturerŐs instructions. SMRTbell template was loaded into the Blue Pippin (Sage Science) to select 12-50 kb double-stranded DNA fragments. Size-selected SMRTbell library was submitted to a final repair DNA damage treatment (SMRTbell Damage Repair Kit, Pacific Biosciences). The library was sequenced using a PacBio RS II platform (P6-C4 chemistry) at the DNA Technologies Core Facility at UC Davis.Ę