High throughput sequencing of phage display library to profile active site specificity of protease ADAMTS13
This project involves high throughput sequencing of phage display libraries to profile active site specificity of proteases ADAMTS13 and Thrombin. Sequencing library was constructed as previously described: Kretz, C.A., et al., Massively parallel enzyme kinetics reveals the substrate recognition landscape of the metalloprotease ADAMTS13. Proc Natl Acad Sci U S A, 2015. 112(30): p. 9328-33