WGS Bacteroides pyogenes from cow
Bacteroides pyogenes were individually cultivated on Wilkins-Chalgren Anaerobe Agar with G. N. Spore Anaerobic Supplement for 48 hours at 37 C in anaerobic conditions and harvested for genomic DNA extraction. Genomic DNA extraction was carried out using the DNeasy blood and tissue kit (Qiagen), according to the manufacturers instructions. Nextera XT kit (Illumina, Inc.) was used to prepare the library according to the manufacturers instructions. The library was loaded into MiSeq Reagent Kit V2 and sequencing was performed using the Illumina MiSeq (Illumina, Inc.), with a 2  250-bp 500-cycle cartridge.