Charmshahr Tannery Wastewater
Water sample was filtered through 0.22 m membrane filters, stored at -20C,  DNA was extracted from membrane filters using a FastDNA Spin Kit,  quality of the extracted metagenomic DNA was checked on 0.8% agarose gel electrophoresis,  its quantity and purity were assessed using Nanodrop spectrophotometer (Thermo Scientific, Wilmington, DE, USA)