GSM3938814: Sample 12; Homo sapiens; miRNA-Seq
Venous blood samples (5ml) were collected in non-anticoagulant acquisition tubes one day before prostate biopsy for serum harvest (1ml) after coagulation.The blood was centrifuged at 2000 g for 10 min at 4 °C to extract the serum. The serum was stored at -80°C till processed.. Illumina TruSeq RNA Sample Prep Kit was used with 1 ug of total RNA for the construction of sequencing libraries.One milliliter serum was used to isolate EV for each sample. After unfrozen in a 25 °C water bath, the serum was centrifuged at 2000 g for 30 min at room temperature to eliminate residual cell fragments. The serum EV was extracted using Total Exosome Isolation Kit (from serum, Thermo fisher scientific) according to the manufacturer's protocol. After extraction, Exosome Purification and RNA Isolation Kit (Norgen Biotek) was employed to purify EV and extract total RNA in EV consequentially.Small RNA libraries were set up with 200 ng EV-delivered total RNAs for each sample. Illumina HiSeqTM 2500 platform was used for deep sequencing assay. miRNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM3938814