GSM2760529: GHSROS-LNCaP rep 1; Homo sapiens; RNA-Seq
At end point, the primary tumour was resected, divided in half; snap frozen and stored at -80°C. Total RNA was extracted from cell pellets using an RNeasy Plus Mini Kit (QIAGEN, Melbourne, Australia) with a genomic DNA (gDNA) Eliminator spin column, according to the manufacturer’s instructions. RNA purity was analysed using the Agilent 2100 Bioanalyzer, and RNA with an RNA integrity number above 7 used for RNA-seq. RNA libraries were prepared for sequencing using standard Illumina protocols (TruSeq stranded mRNA library)
GEO Accession
GSM2760529