GSM1633725: 13902-KO-413_Bcell_nostim; Mus musculus; RNA-Seq
B cells were purified from miR-212/132-/- and WT mice and subsequently either left unstimulated, or stimulated for 8 hours or 20 hours with anti-IgM (5µg/mL). RNA was then harvested from these cells using the miRNAeasy RNA prep kit (Qiagen). To isolate B cells, RBC depleted bone marrow or spleen samples were sorted on MACS columns (Miltenyi). Briefly, bone marrow or spleen cells were incubated first with biotin-conjugated CD19 (positive-selection) or CD43 (negative selection) antibodies (BioLegend) in MACS sorting buffer (Miltenyi), followed by incubation with streptavidin-conjugated magnetic beads (Miltenyi) as per manufacturers protocol, before being separated on the columns. TruSeq RNA Sample Preparation Kit
GEO Accession
GSM1633725