GSM1531258: oblong_polyA_RNA_Seq; Danio rerio; RNA-Seq
Mouse samples Total RNA was extracted with the miRNeasy kit's procedure (Qiagen). For preparation of RNA-Sequencing samples, we used 10 μg total RNA and removed rRNA with the RiboZero kit (Epicenter). After taking an aliquot of 100 ng ribosomal depleted total RNA, we followed with 4sU purification for the remainder of the sample as desceibed in Rabani et. al., Nat. Biotech., 2011. Zebrafish samples Total RNA was isolated using the standard Trizol (Invitrogen) protocol. Two rounds of PolyA+-RNA purification were performed for each sample, using the PolyA(Purist)TM-MAG kit (Ambion). The quality of the RNA and lack of contaminating ribosomal RNA were confirmed using the Agilent 2100 Bioanalyzer. We prepared the RNA-Seq libraries using the dUTP second strand (strand specific) protocol as described in Levin et. al, Nat. Methods, 2010.
GEO Accession
GSM1531258