Illumina MiSeq sequencing; qiita_ptid_2602:10863.243.5
This project uses the novel WinCF model to test the effect of an oxygen gradient and antibiotic treatment on the structure and function of the cystic fibrosis lung microbiome
Illumina EMP protocol 515fbc, 806r amplification of 16SrRNA V4
barcode
AGCTTCGACAGT
center_name
UCSDMI
extraction_robot
HOWE_KF3
extractionkit_lot
154050095
linker
GT
mastermix_lot
396036
orig_name
243.5
pcr_primers
FWD:GTGYCAGCMGCCGCGGTAA; REV:GGACTACNVGGGTWTCTAAT
plating
LDG
primer
GTGTGYCAGCMGCCGCGGTAA
primer_date
1/19/17
primer_plate
6
processing_robot
RIKE
project_name
CF_Sputum_RobQ
run_center
UCSDMI
run_date
3/13/17
run_prefix
RQCF_Sputum_S2_L002
sample_plate
CF_Sputum_RobQ_5
sequencing_meth
Sequencing by synthesis
target_gene
16S rRNA
target_subfragment
V4
tm1000_8_tool
108379Z
tm300_8_tool
311318B
tm50_8_tool
311426B
water_lot
RNBF7110
well_description
CF_Sputum_RobQ_5_243.5_A5
well_id
A5