454 Reference for Carlton variety (basal plate) second sample
RNA was extracted using a modified CTAB method developed for pine trees (Chang et al, 1993) followed by RNA clean up from Qiagen RNeasy plant mini kit, mRNA was selected using Dynabeads mRNA purification kit. cDNA library was produced using the cDNA Rapid Library Preparation method (Roche) and labelled with MID6 adaptor (Roche).