RNA-seq of Meiacanthus atrodorsalis: venom gland tissue pooled from ten specimens
RNA extracted using the TRIzol Plus RNA Purification Kit (Life Technologies). RNA quality was assessed using a Bioanalyser (Agilent) and ribosomal RNA removedusing the Ribo-Zero rRNA Removal Kit for Human/Mouse/Rat (epicentre). The RNA-Seq library was prepared from 50Ęng of the enriched RNA using the ScriptSeq v2 RNA-Seq Library Preparation Kit (epicentre), following 12Ęcycles of amplification. The sequencing library was then purified using AMPure XP beads (Agencourt), quantified using the Qubit dsDNA HS Assay Kit (Life Technologies) and the size distribution assessed using a Bioanalyser (Agilent). The library was multiplexed and sequenced on a single lane of an Illumina MiSeq machine housed at the Centre for Genomic Research (Liverpool, UK).