MiSeq 2x300bp library
MiSeq libraries were prepared following a standard Illumina DNA library preparation protocol, which involved shearing of the DNA by sonication (Covaris S2) to 550 bp fragments, XP bead purification (Beckman Coulter), end polishing, A-tailing and ligation of indexed Illumina adapters (Illumina TruSeq DNA PCR-Free kit), depletion of adapters by XP bead purification (Beckman Coulter), and library quantification by qPCR with the KAPA library quantification Kit (KAPA Biosystems)