GSM4678196: A549-CRISPR cells_PA14_rep2; Homo sapiens; OTHER
After three rounds of infection, the surviving populations were expanded to obtain 20.106 cells. Uninfected A549-CRISPR cells were expanded similarly. Each cell population was subjected to genomic DNA (gDNA) extraction with a QIAamp DNA Blood Maxi kit (Qiagen). A one-step PCR was carried out to enrich and amplify gRNAs from the gDNA of selected cell populations with Illumina TruSeq adapters and a unique barcode of two 8-bp index required for Illumina sequencing. The resulting amplicons were gel extracted, quantified and sequenced using a NextSeq 500 (Illumina).
GEO Accession
GSM4678196