Equine encephalosis virus 7 strain 7/Labstr/ZAF/2000/St. Lucia E21/20 Viral Amplicon Sequencing
The sequencing library was prepared using the Pacific Biosciences (PacBio) protocol for preparing multiplexed microbial SMRTbell libraries, whole-genome sequencing was achieved in a 8-plex library using a long-read PacBio single-molecule real-time (SMRT) sequencing platform, according to the protocol Preparing Multiplexed Microbial Libraries Using SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences, Menlo Park, CA, USA) but with the exception of DNA shearing. The library was sequenced on a PacBio Sequel instrument with on-plate concentration of 4 pM and a 10-hour movie time using v3.0 chemistry, Sequel Polymerase v3.0 and SMRT cells v3.  Circular-consensus sequencing (CCS) analysis was performed using PacBio SMRT link version 6.