RNA-Seq fom anthers of the barley cultivar Golden Promise
At least 120 anthers of 1.3  1.4 mm size were collected and RNA was extracted using TRIzol PlusRNA Purification Kit (Thermofisher Scientific) following the manufacturers instructions). RNA-seq libraries were constructed using TruSeq mRNA Sample Preparation kit (Illumina) as recommended by the manufacturer. Libraries were quality checked using a Bioanalyzer 2100 (Agilent) and quantified by both qPCR (KAPA Library Quantification Kit, KAPA Biosystems) and using a Qubit fluorometer.