GSM4679515: BNeutsBld27; Homo sapiens; RNA-Seq
Neutrophil subpopulations were FACS sorted from human fresh bone marrow and melanoma blood Cells will be sorted directly in TriZOL LS and frozen at -80 immediately. Sorted bone marrow cells and melanome blood in TRIzol LS Reagent were thawed on ice. RNA isolation was performed using the Direct-zolTMRNA MiniPrep (Zymo Research) according to manufacturer's instructions. RNA concentration was measured on a Agilent 2200 TapeStation using the Agilent High Sensitivity RNA ScreenTape System (Agilent Technologies). Library preparation and sequencing will be performed according to the Smart-Seq2 protocol in the La Jolla Institute for Immunology sequencing core.
GEO Accession
GSM4679515