GSM1531423: Aebp2-WT-Thymus-ChIP-seq; Mus musculus; ChIP-Seq
Genomic DNA was isolated from thymus using standard phenol-chloroform extraction protocol, precipitated using standard ethanol precipitation protocol and resuspended in 1X TE buffer. Thymus tissues of one-month-old C57BL/6N mice (Taconic) were used for ChIP experiments as previously described [Kim et al. 2009. Nucleic Acids Res.].  This particular protocol has one additional step compared to other existing ChIP protocols. The two four base pair (bp) cutter enzymes, AluI and Tsp509, were used to shorten the average length of ChIP DNA right before the elution step from the AEBP2 antibody.  The eluted DNA was further processed for the construction of a library according to the manufacturer’s protocol (NEBNext Fast DNA Library Prep Set for Ion Torrent, NEB cat. No. E6270S). The constructed library was sequenced using a NGS sequencer (Ion Torrent PGM, Life Technologies).
GEO Accession
GSM1531423