2C short fragment library 170bp
Genomic DNA extracted with CTAB method and fragmented using Coveris S3 instrument. Library was prepared following Illumina TruSeq protocol starting from 3ng of DNA. Fragments were selected on agarose gel prior PCR enrichment step to target an average fragment size of 170bp and obtain overlapping pairs (selection at 300bp). PCR enrichment was carried out with 5 cycles. This library was constructed with the aim to obtain high coverage sequencing (>40x) for this sample to satisfy requirements of Allpaths-LG software.