Escherichia fergusonii GTA-1504 5 RBA P2
Isolates were cultured on nutrient agar (Difco, Be cton, Dickinson & Co) overnight (14-16 hrs) at 37°C , and genomic DNA was extracted from single colonies using the Maxwell® 16 Cell LEV DNA Purification kit (Promega, Madison, WI). DNA was quantified usin g the Quant-iT High-Sensitivity DNA Assay Kit (Life Technologies Inc., Burlington, ON). Sequencing libr aries were constructed from 1 ng of gDNA using the Nextera XT DNA sample preparation kit (Illumina, In c., San Diego, CA) and the Nextera XT index kit (Illumina, Inc.).