WGS of Candida albicans pmr1::FRT/pmr1::FRT cell wall mutants
A single colony was picked from a YPD-agar plate and grown overnight in 10 ml of YPD at 37 degrees Celsius under orbital shaking at 150 rpm. 1 ml of overnight culture was harvested by centrifugation at 3500 rpm for 5 minutes. Genomic DNA was extracted using the phenol-chloroform-isoamylalcohol method as described in G. Rancati et al., Cell 2008 [Pubmed ID:    19041751]. Libraries were prepared using Illumina TruSeq® DNA sample preparation kit version 2 (Low-Throughput protocol) according to manufacturer's protocol except that size selection were done using E-Gel® SizeSelect™ 2% pre-cast agarose gels.