GSM1634469: Hira-KO5_RNA-Seq; Mus musculus; RNA-Seq
cDNA synthesis and amplification was performed directly on single Hiraf/f and Hiraf/f Gdf9-Cre+ MII oocytes with the SMARTer Ultra Low Input RNA kit (Clontech). 0.5 uL of ERCC RNA spike-in mix 1 (Life Technologies), diluted 1:105, was added to each reaction prior to cDNA synthesis. The amplified cDNA was fragmented by Covaris S2 sonicator (Covaris) and converted to sequencing libraries following the NEBNext DNA Library Prep (NEB) using the NEBNext Multiplex Oligos for Illumina (NEB). Bar-coded libraries were pooled and sequenced on two lanes of the Illumina HiSeq 2500 instrument.
GEO Accession
GSM1634469