454 GS Junior sequencing
Analysing the BR repertoire in HD of different ages
cDNA from the total PBMC�s was amplified using forward VH1-6 FR1 (BIOMED-2) primers and either the CgCH1 or the IGHA reverse primer. Samples were purified by gel extraction and AMPure XP beads and sequenced using 454 GS junior sequencing with the Lib-A V2 kit. Data was processed using the amplicon 1 pipeline from Roche. Samples were demultiplexed and filtered using IGGalaxy and divided in Cg and Ca transcripts as described in IJspeert et al Front. Immunol. 7:410. doi: 10.3389/fimmu.2016.00410 are deposited.. The deposited data are untrimmed. In the analysis of IJspeert et al samples were 40nt trimmed before analysis