GSM3175136: MC1-CD44+/CD24-; Homo sapiens; RNA-Seq
FACS sorted cells were pelleted and flash frozen in 50ul PBS.  Total RNA from the frozen cell pellets were extracted using Norgen Single Cell RNA Purification Kit (catalog number 51800) as per manufacturer protocol. Quality of RNA was determined using Agilent BioAnalyzer with the Eukaryote total RNA Pico assay kit.  RNA libraries were prepared with 10ng total RNA as input using the Nugen Ovation RNASeq V2 library kit (cat# 7102-08) along with Kapa LTP library kit cat# KK8230). RNA libraries were prepared as per standard manufacturer protocol
GEO Accession
GSM3175136