GSM3174203: F_1: Inguinal adipose tissue; Mus musculus; RNA-Seq
Adipose tissues were washed with PBS, minced, and digested with type II collagenase (2 mg/ml) in KRBB containing 10 mM HEPES (pH 7.4), and 3% BSA for 1 hr at 37°C.Digested tissue was successively filtered through 70-μm and 40-μm cell strainers (BD Biosciences) and then centrifuged with 500g for 5min. The cell pellet was resuspended with PBS. Tomato+ cells were sorted by Bio-Rad S3e and then single cells were picked with fluorescence microscope. Libraries were prepared according to Nextera® XT DNA Library Prep Kit for Illumina  (Cat# FC-131-1096) RNA-seq (SMART-Seq v4 3' DE Kit)
GEO Accession
GSM3174203