2-3kb_repeat3; Phyllostachys edulis; RNA-Seq
Three different libraries size were constructed and each library was sequenced on two SMRT cells. Total RNA from bamboo was used as starting material to synthesize first-strand cDNA.Size selection was done with the BluePippin™ cassette for 3 size fractions, 1–2 kb, 2 –3 kb and above 3kb in order to capture the broadest range of transcripts. Following size selection, a second round of PCR is performed to generate sufficient DNA for SMRTbell template preparation.