RNA-Seq of drosophila melanogaster: adult female ovary
Total RNA from testes, ovaries, and salivary glands were separately isolated with TRIzol. Sample quality was verified on an Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA, USA), and RNA was quantitated on an ABI StepOnePlus Real-Time PCR System (ABI, Foster City, CA, USA). RNA was sequenced on an Illumina HiSeq 2000 at the BGI