GSM4504493: ChIRP-Seq data for Lnc-CCTT in hela cells replicate1; Homo sapiens; OTHER
Each sample of beads were resuspended in 150 μL of DNA Elution Buffer (50 mM NaHCO3, 1% SDS) with RNases (1 0μL RNase A (10 mg/mL) and 10 μL RNase H (10 U/μl) per ml of DNA Elution Buffer) and DNA INPUT was resuspended in 140 μL. Samples were Incubated at 37 °C for 30min with shaking. Beads and supernatant were separated on DynaMag-2 magnetic strip. Repeat the previous step. All supernatant (should be ~300 μL) was collected and added 15 μL PK. Samples were incubated at 50 °C for 45 min with shaking. DNA samples were transfered to phase-lock gel tubes and followed protocols from Qiagen. Strictly follow protocols from NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®.
GEO Accession
GSM4504493