RNA-Seq of mouse: adult colon
Total RNA was extracted from tissue samples using MagMAX kit (Life tech, Carlsbad, CA) following the suppliers provided protocol. Strand-specific RNA-seq libraries were prepared from 500 ng RNA using KAPA stranded mRNA-Seq Kit (KAPA Biosystems). The libraries were amplified by twelve-cycle PCR. Sequencing was performed on Illumina HiSeq2000 (Illumina) by multiplexed single-read run with 33 cycles.