MeRIP-seq of skin-FT
After the total RNA was extracted with Trizol reagent, the total RNA with Polya mRNA was enriched with oligo DT magnetic beads. The magnetic beads were enriched to obtain Polya mRNA. After that, the complete mRNA was segmented by adding fragment reagent, and the length of fragment was about 100nt. An immunomagnetic bead with pre mixed m6A antibody was added to enrich the mRNA fragment containing m6A methylation. After enrichment of m6A antibody immunomagnetic beads and recovery of the mRNA fragment with m6A, the conventional sequencing library was constructed according to the construction process of transcriptome.