DNA-Seq of Sphenodon punctatus: adult male blood: 2x250 PE 400 insert
Total genomic DNA was extracted using proteinase K digestion and Phenol-Chloroform extraction from blood. Sequencing was undertaken using the Illumina MiSeq sequencing platform (Illumina, San Diego, CA, USA). Sequencing libraries consisted of paired end (PE) libraries with estimated insert sizes of 400 bp. The paired end libraries were prepared using the Illumina Kappa DNA library kits as per manufacturers instructions. These libraries were normalised and pooled across 4 lanes of Illumina MiSeq using 2 x 250 bp paired end sequencing at New Zealand Genomics Ltd., Dunedin.