GSM4568376: HiChIP, My-La CD8+ T lymphocyte cells; Homo sapiens; OTHER
Cells were thawed on ice and re-suspended in 50ml ice-cold lysis buffer (10mM Tris-HCl pH 8, 10mM NaCl, 0.2% Igepal CA-630, one protease inhibitor cocktail tablet). Cells were lysed for 30 minutes at 4C with rotation. The nuclei were permeabilised and the chromatin was digested with MboI. Ends were marked with biotin followed by in-nucleus ligation, shearing by sonication and immunoprecipitation with an antibody for H3K27ac (Abcam ab4729). De-crosslinking, biotin pulldown and tagmentation was conducted according to the previously established HiChIP protocol (Mumbach et al., 2016) Sequencing library was generated with 10 cycles of PCR using Nextera dual index primers and Phusion HF 2X (NEB)
GEO Accession
GSM4568376