Amplicon Sequencing of Marchantia polymorpha fungal endophytes
Libraries were prepared in three separate PCR stages. First, all samples were amplified using standard primer pairs for 15 cycles to enrich for the target region. Second, sequencing adaptors were added in 10 cycles of PCR. Adaptor primers in this step were mixes of six frameshifted primers to increase sequence diversity. Third, unique barcodes and adaptors were added to each sample in a final 10 cycle PCR.