GSM3938121: spleen_0; Mus musculus; RNA-Seq
Single cells were extracted from suspensions of spleen from 3 female individual litter-mate mice that were pooled for spleen_0 sample and individual female litter-mate mice for spleen_1 and spleen_2 sample  (1 per sample) For Spleen_1 and Spleen_2 samples, mice were intravenously injected with 25μg of commercial (BioLegend, #149802) NICD-protector 15 minutes prior to organ harvest. Cells were sorted by flow cytometry (using a FACSAria III, BD Biosciences) and gated for dump-CD4+CD44+gp66+ expression. The sorted cell suspension from each sample was loaded into a single well of a 10× Genomics Chromium Single Cell Controller. Single-cell capture and cDNA and library preparation were performed with a Single Cell 3' v2 Reagent Kit (10× Genomics) according to manufacturer's instructions.