GSM4190177: K562 [PacBio]; Homo sapiens; OTHER
RNA was extracted with Trizol and Direct-zol RNA kit from Zymo Research. PacBio: Selected cDNA were PCR amplified using gene specific primers with containing following common sequences: GTGACTGGAGTTCAGACGTGTNNNNNNNN and ACACTCTTTCCCTACACGAC. PCR products were purified and pooled. Pooled PCR products were used for library generation using SMRTbell™ Template Prep Kit 1.0‐SPv3 and sequenced on PacBio Sequel system.
GEO Accession
GSM4190177