GSM4628288: rab7d gene-silenced strain rep 3; Entamoeba histolytica; RNA-Seq
Logarithmic growth phase of E. histolytica trophozoites were harvested then total RNA was extracted using Trizol reagent. RNA-seq libraries were generated by using TruSeq stranded mRNA kit. The protocol included polyA plus RNA purification, RNA fragmentation, random hexamer primed cDNA synthesis, linker ligation, PCR amplification, and gel purification. The libraries were then subjected to 100 nt paired-end sequencing using an Illumina HiSeq2500.
GEO Accession
GSM4628288