RNAseq of Arabidopsis thaliana dormancy cyling seed August_rep3
RNA was isolated from c. 150 seeds using the Hot Borate protocol (Wan & Wilkins 1994). RNA quality and quantity was assessed with the Trinean Xpose machine. Preparation of the libraries and Illumina-sequencing was performed by the in-house sequencing facility. 500-1000 ng RNA was used for poly-A library preparation. Sequencing length was 50 nt, the read library was single-end.