Nipponbare RNAseq replicate3. Comparative transcriptome analysis among closely related rice strains
Seeding shoot of two weeks rice plants were harvested to extract total RNA using RNAeasy Mini RNA kit (Qiagen). Total RNA was digested with DNase in room temperature and purified using RNAeasy Mini RNA kit (Qiagen). The purified RNA was fragmented and cDNA was synthesized using Superscript II with random primers (Life technologies). Adaptors were linked to cDNA template and fragments of 200 bp were selected by agarose gel electrophoresis. Three biological replicates were made for each of NB, HEG4 and EG4. Libraries were sequenced for 51 bp single-end on Hiseq 2000 (Illumina).