GSM3755272: DD003_A217; Mus musculus; RNA-Seq
Liquid medium was removed from the organoid-containing Matrigel mounds. 500ul of Trizol was added to the wells and triturated to dissolve the organoids and Matrigel. Trizol was stored at -80C before RNA isolation was performed using the RNAeasy PLUS kit according the the manufacturer's instructions. RNA samples with a RIN value of 9.8 - 10 were used. Performed by the JP Sulzberger Columbia Genome Center: protocol 133, Ribo-zero 60M PE100. Ribosomal RNA was depleted using the Ribo-zero kit and libraries prepared using the Illumina TruSeq RNA prep kit.
GEO Accession
GSM3755272