GSM3559627: RNA-seq_WT_rep4; Mus musculus; RNA-Seq
Total RNA from four embryos (e13.5, 2 males and 2 females) of either WT or Dlx1/2-/- was purified using RNeasy Mini Kit (Qiagen). The DNAse treated RNA was checked on Bioanalyzer and concentration was measured. RNA sequencing libraries were generated using TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Mouse, according to the manufacturer's protocol (Illumina, San Diego, CA, USA). Fragment size distribution was assessed using the Bioanalyzer 2100 and the DNA high-sensitivity chip (Agilent). Concentration of the libraries was measured using the Kapa library quantification kit (Kapa Biosystems, Woburn , MA, USA). Libraries were multiplexed, sequenced on the HiSeq 4000 and generated, roughly 814 million single-end 50bp reads.
GEO Accession
GSM3559627