GSM4567419: spleen, anti-IL6R_3; Mus musculus; RNA-Seq
Total RNA extracted using RNEasy mini kit (Qiagen) according to manufacturer instructions 0.1 μg of total RNA was used as an input material for library preparation using TruSeq Stranded Total RNA Library Prep Kit (Illumina, San Diego, CA). Size of the libraries was confirmed using 4200 TapeStation and High Sensitivity D1K screen tape (Agilent Technologies, Santa Clare, CA) and their concentration was determined by a quantitative PCR-based method using Library quantification kit (KAPA Biosystems, Wilmington, MA). The libraries were multiplexed and sequenced on Illumina HiSeq4000 (Illumina, San Diego, CA) to generate 30 million single-end 50 base pair reads.
GEO Accession
GSM4567419