GSM1533403: input DNA; Mus musculus; ChIP-Seq
Isolated chromatin was sonicated and pre-cleared with isotype-matched rabbit non-specific IgG. Chromatin immunprecipitation was carried out with a GR antibody (Santa Cruz, sc-8992) or a PPARalpha antibody (Santa Cruz, sc-9000). 107 cells were used per IP condition. The formaldehyde crosslinks were reversed by heating to 65-degrees for 4 hours. RNA and protein were removed by RNAse A and Proteinase K treatment, and the DNA was isolated using phenol-chloroform extraction. Libraries were prepared according to Illumina's standard protocol, with instructions accompanying the DNA Sample Kit. Immunoprecipitated DNA, along with input control, was prepared for sequencing with the Illumina TruSeq DNA Sample Prep kit according to the standard protocol with the following revisions. A 50-fold dilution of the Adapter mix was used in the ligation reaction. Additionally, the final libraries were size selected for fragments between 200 and 400 bp with a Pippin Prep system prior to sequencing.
GEO Accession
GSM1533403