454 GS Junior sequencing
Analysing the BR repertoire in HD of different ages
cDNA from the total PBMC�s was amplified using forward VH1-6 FR1 (BIOMED-2) primers and either the CgCH1 or the IGHA reverse primer. Samples were purified by gel extraction and AMPure XP beads and sequenced using 454 GS junior sequencing with the Lib-A V2 kit. Data was processed using the amplicon 1 pipeline from Roche.