GSM2838958: T7-25_CENPA; Schizosaccharomyces pombe; ChIP-Seq
Lysates were prepared using standard bead beating procedure, sonicated to achieve fragmements of 200-400bp andclarified by centrifugation at 17000g. Libraries were prepared using standard Illumina pipeline. Briefly, DNA was end-repaired using NEB Quick blunting kit (E1201L).  The blunt, phosphorylated ends were treated with Klenow fragment (3' to 5' exo minus) and dATP to yield a protruding 3- 'A' base for ligation of NEXTflex adapters (Bioo Scientific) which have a single 'T' base overhang at the 3' end. After adapter ligation DNA was PCR amplified with Illumina primers for 13-15 cycles and library fragments of ~300 bp (insert plus adaptor and PCR primer sequences) were selected using Ampure XP beads. The purified DNA was captured on an Illumina flow cell for cluster generation.
GEO Accession
GSM2838958