GSM2339847: 16_1_Basal; Homo sapiens; RNA-Seq
Total RNAs were purified from skeletal muscle biopsies with the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen) following manufacturer’s instructions. The quality of recovered RNA was assessed by using the Agilent RNA 6000 Nano Kit (Agilent Technologies). RNA concentration was determined by spectrophotometry with a NanoDrop 2000. After purification, RNA samples were stored at -80°C until further analysis Libraries were prepared after ribosomal RNA depletion with the TruSeq Stranded Total Sample Prep Kit with Ribo-Zero Gold (Illumina). Sequencing was done on a HiSeq 2500
GEO Accession
GSM2339847