HTGS of Rhopilema esculentum: ectoderm tissue
Genomic DNA was extracted from the ectoderm tissue using TIANamp Marine Animal DNA Kits (Tiangen, Beijing, China) and used for sequencing. For PacBio library construction, genomic DNA of R. esculentum was sheared to ~20 kb, and the short fragments below the size of 7 kb were filtered using BluePipin (Sage Science, MA, USA). Filtered DNA was then converted into the proprietary SMRTbell library using the PacBio DNA Template Preparation Kit.