GSM3619563: RU3; Cynoglossus semilaevis; RNA-Seq
The fish were sampled before injection and 24 hpi, and the liver, spleen, kidney tissues were collected from three fish each group, immediately frozen in liquid nitrogen. mRNA was purified from the mixed 3 µg total RNA using poly-T oligo-attached magnetic beads cDNA libraries were constructed using NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer's recommendations.
GEO Accession
GSM3619563