GSM2584481: WT SFVmac vector in HT1080 cells; Homo sapiens; OTHER
Cellular genomic DNA isolated 5 days post-infection was digested with BfaI/BanII/CviQI and ligated to a linker made by annealing oligonucleotides 5´- TACTGAGACGTCGATGC (with 3´ end blocked by an amino group modifier) and 5´-GATCATGCGAGATACATCTCAGGCATCGACGTCTCAG. SFVmac integration sites were amplified using primers 5´- CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCTGATCATGCGAGATACATCTCAG and 5´- GTGGGTACTACACTTATCATAAGAGG followed by a nested PCR with primers 5´- CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCTGATCATGCGAGATACATCTCAG and 5´- AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTXXXXXXGTTCCTAAGGATAATCAATACACAATATTC.
GEO Accession
GSM2584481