GSM1635750: C8d rep1; Phaeodactylum tricornutum; RNA-Seq
Total RNA was extracted from P. tricornutum cells using the Trizol method according to the manufacturer’s instructions (Invitrogen). Extracted RNA was treated with TurboDNase I (Life Technologies AM2238) according to product instructions and the treated RNA was purified using and RNeasy spin column (Qiagen) with 0.5 volumes of ethanol, washed and then eluted with 50 μL room temperature molecular grade water (Qiagen). The quality of the purified RNA was assessed using the Agilent 2100 Bionanalyzer. cDNA libraries for sequence-specific RNA Sequencing (ssRNA-seq) were prepared using the Illumina TruSeq Stranded mRNA Sample Preparation Kit following the Low Sample (LS) Protocol guidelines. The quality and size of the libraries from each sample was tested using an Agilent High Sensitivity D1K ScreenTape System. Samples were pooled into three wells (three to five samples total per well were used) and sequenced on an Illumina HiSeq 2500 with 100-cycle single reads per multiplexed sample. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM1635750