Illumina HiSeq 2000 sequencing; Transcriptional Profiling of human pluripotent stem cells and and derived tissues
Transcriptional Profiling of human pluripotent stem cells and and derived tissues
Definitive endoderm was generated by treating H9 embryonic stem cells with Activin A for 3 days as previously described by Spence, et al. Nature 470: 105-109 (2010) and McCracken, et al. Nature Protocols 6:1920-1928 (2011). RNA was extracted from samples and then prepared for RNAseq using the Illumina TruSeq mRNA method
Experimental Factor: cell type
definitive endoderm differentiated from H9 stem cells
LIBRARY_STRAND
not applicable