Illumina HiSeq 2000 sequencing; Transcriptional profiling of esg+-midgut progenitor cells expressing esgRNAi or UAS-esg AND of whole midgut tissue from flies overexpressing UAS-esg in enterocyte (EC) cells
Transcriptional profiling of esg+-midgut progenitor cells expressing esgRNAi or UAS-esg AND of whole midgut tissue from flies overexpressing UAS-esg in enterocyte (EC) cells
MyoIAts was used to drive transgene expression for 24 hours at 29 degrees C. 25 adult female intestines per genotype were dissected and midguts were homogenized in Trizol (Life Technologies). RNA was isolated by Phenol/Chloroform extraction, followed by purification of the RNA containing fractions using RNAeasy columns supplied with the RNAeasy Mini Kit (Qiagen). RNA was Poly-A-selected. RNA integrity was determined with an Agilent 2100 Bioanalyzer before library generation. cDNA libraries were generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB), and NEBNext Multiplex Oligos for Illumina (NEB) were used for multiplexing.
Experimental Factor: genotype
UAS-esg overexpression
LIBRARY_STRAND
not applicable