Illumina HiSeq 2000 paired end sequencing; Analysis of abomasal lymph node transcriptome for the identification of genes and lncRNAs involved in nematode resistance gastrointestinal tract in adult sheep.
RNA-Seq transcriptomic paired end experiment using Illumina HiSeq 2000 sequencer
KAPA Stranded mRNA-Seq Illumina(r) Platforms Kit
assay type
RNA-seq of total RNA
sample storage
RNAlater, frozen
sample storage processing
cryopreservation in liquid nitrogen (dead tissue)
sampling to preparation interval
12
experimental protocol
https://doi.org/10.1186/s13567-018-0534-x
extraction protocol
https://doi.org/10.1186/s13567-018-0534-x
library preparation location
Barcelona, Spain
library preparation location longitude
41.390205
library preparation location latitude
2.154007
library preparation date
2017-01-16
sequencing location
Spain
sequencing location longitude
41.390205
sequencing location latitude
2.154007
sequencing date
2017-02-10
experiment target
total RNA
rna preparation 3' adapter ligation protocol
http://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf
rna preparation 5' adapter ligation protocol
http://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf
library generation pcr product isolation protocol
http://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf
preparation reverse transcription protocol
http://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf
library generation protocol
http://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf
read strand
mate 2 sense
rna purity - 260:280 ratio
1.975
rna purity - 260:230 ratio
0.7
rna integrity number
7.8