GSM3755689: Short-term PD rep3; Homo sapiens; RNA-Seq
About 10,000 cells were loaded to each channel and then partitioned into Gel Beads in emulsion in the Chromium instrument, where cell lysis and barcoded reverse transcription of RNA occurred, followed by amplification, fragmentation and 5' adaptor and sample index attachment. 1μl of sample was diluted 1:10 and ran on the Agilent Bioanalyzer High Sensitivity chip for qualitative analysis. Libraries were sequenced on an Illumina HiSeq4000 (Illumina) using a custom paired-end sequencing mode (26 bp for read 1 and 98 bp for read 2). Single cells were processed through the Chromium Single Cell 3' Solution platform using the Chromium Single Cell 3' Gel Bead, Chip and Library Kits (10X Genomics) following the manufacturer's protocol.
GEO Accession
GSM3755689