Human, TG003 treatment, replicate 1
Total RNA was extracted from hSkMC and C2C12 cells with Sepasol  (Nacalai Tesque). 5 µg of total RNA was poly-A purified by ambion Dynabeads mRNA DIRECT Micro Kit (Life Technologies), and 45 ng of poly-A purified RNA were used for RNA-seq library preparation using Ion Total RNA-seq Kit v2 (Life Technologies). Single-end sequencing was performed on Ion Proton System.