S. oleracea TruSeq rep2
Leaves of S. oleracea were disrupted and mixed with Trizol. Total RNA library preparation was carried out according to the Illumina TruSeq small RNA library preparation protocol. A pre-adenylated 3'-adapter was incubated with total RNA in the presence of T4 RNA ligase 2, while the 5'-ligation was carried out with T4 RNA ligase 1 and a 5'-RNA adapter. Reverse transcription and cDNA amplification was carried out according to the manufacturers protocol (Illumina). High-throughput analysis of the libraries was done as single end run (150 nt) on a Illumina MiSeq System with the custom primer (5'-CACTGTCGGTACCGAGCTTGCATGGAGTCCTA-3'). High-throughput analysis of the libraries was done as single end run (150 nt) on a Illumina MiSeq System with the custom primer (5'-CACTGTCGGTACCGAGCTTGCATGGAGTCCTA-3').