GSM4628209: Non-epithelial mammary cells - high-fat diet_rep1; Mus musculus; RNA-Seq
Non-epithelial mammary cells isolated from mammary glands were enriched by removing epithelial cells using the anti-EpCAM antibody and magnetic dynabeads. To isolate viable cells, enriched non-epithelial cells were stained with 7-AAD dye (0.5 µg/ml) and 7-AAD-negative viable cells were sorted using the FACS Aria II cell sorter (BD Biosciences, Franklin Lakes, NJ, USA). FACS-sorted viable single cells were processed by using the 10X Genomics Chromium device to prepare scRNA-seq libraries (Single Cell 3' v2) according to the manufacturer's protocol. Single-cell RNA-seq (scRNA-seq)
GEO Accession
GSM4628209