GSM4191888: Experimental cDNA replicate 3 ("13") for MPRA1; Pan troglodytes; Homo sapiens; OTHER
Inert and competent libraries were sequenced from plasmid PCR amplicons. Experimental libraries were extracted from NSCs using Qiagen Allprep and MinElute PCR Purification kits (pDNA, 5% of each sample) and Qiagen RNeasy Mini kit (cDNA, 95%). cDNA was synthesized using Invitrogen SuperScript III reverse transcription kit and using a mixture between a custom, library-specific primer and oligo-dT primers (MPRA1) or only custom primer (MPRA2). We amplfied the library amplicon using qRT-PCR to stop amplification in the exponential phase. pDNA and cDNA libraries were then paired-end sequenced for 150 cycles on an Illumina HiSeq 4000s (MPRA1) or NovaSeq 6000s (MPRA2), including 5% phi-X spike-in.
GEO Accession
GSM4191888