GSM3098443: FT1; Gallus gallus; RNA-Seq
Total RNA was isolated from abdominal fat tissue with the RNAsimple Total RNA kit (Tiangen Biotech Inc., Beijing, China) in accordance with the manufacturer's instructions. mRNA was enriched from total RNA samples using oligo (DT) magnetic beads (Invitrogen, USA). Purified mRNA was first fragmented by RNA fragmentation kit (Ambion, USA), then first-strand cDNA was synthesized. Then the second-strand cDNA were synthesized according to the manufacturer's instructions. The cDNA library was assessed with Agilent 2100 Bioanalyzer and ABI Step One Plus Real-Time PCR System.
GEO Accession
GSM3098443