GSM3098518: QHMM rep1 microRNA; Ovis aries; miRNA-Seq
Total RNA was extracted from muscle tissue samples by Trizol Reagent (Life technologies, USA). The RNA quality was checked by NanoDrop 2000(Thermo scientific, USA) and Agilent 2200(Agilent, USA) instruments. The cDNA libraries were prepared for single-end sequencing by using Ion Total RNA-Seq Kit v2.0 (Life technologies, USA) and had been size selected by PAGE Gel electrophoresis for miRNA sequencing. Samples were diluted and processed on a OneTouch 2 instrument(Life Technologies, USA) and enriched on a OneTouch 2 ES station(Life Technologies, USA) for preparing the template-positive Ion PI™ Ion Sphere™ Particles(Life Technologies, USA)according to Ion PI™ Template OT2 200 Kit v2.0(Life Technologies, USA).
GEO Accession
GSM3098518