GSM2583766: E93-44hAPF-FAIRE-Rep1; Drosophila melanogaster; FAIRE-seq
A minimum of 40 wings were dissected from staged female flies in 1x PBS and transferred to ice for subsequent processing. 6 rounds of bead beating (1 minute beating, 2 minutes resting) were performed on dissected tissue. Lysate was sonicated with a Branson sonifier to a fragment size range of 500 bp – 2kb. Soluble chromatin fraction was isolated by centrifugation at 15,000 RCF x 5’ at 4C. Soluble chromatin fraction was phenol-chloroform extracted. Isolated FAIRE-enriched DNA was used for library prep. Rubicon Thruplex DNA-seq kit
GEO Accession
GSM2583766