GSM4191603: HF-Sugar_M3; Mus musculus; RNA-Seq
Ileum was removed and snap frozen in liquid nitrogen. Total RNA from each mouse ileum tissue was isolated individually using RNA queouse-4PCR Kit (Ambion). Quality and concentration of the extracted total RNA using Agilent 2100 Bioanalyzer (Agilent Technologies). Out of the samples with RNA integrity number (RIN) greater than 8, a total of 12 samples (3 samples per group) were randomly selected to be sequenced. Libraries were prepared for sequencing using the Ion Total RNA-Seq Kit v2 protocol (Life Technologies) using an equal total RNA input of 5µg from every sample.
GEO Accession
GSM4191603