SCNT_CNT1
Briefly, mRNAs were first segmented in a fragmentation buffer, followed by first-strand cDNA synthesis. After second-strand synthesis, double-stranded cDNAs were end repaired by T4 DNA polymerase, followed by A-tailing with Klenow DNA polymerases [poly(A)-tail at the 3-end]. AMPure XP beads were used to clean up the libraries, and PCR was performed to obtain the final sequencing library.