16S amplicon of P. kaempferi; bacteriomes  of male
Sequencing libraries were generated using NEBNext Ultra DNA Library Prep Kit following manufacturers recommendations and index codes were added. The library quality was assessed on the Qubit 2.0 Fluorometer (Thermo Scientific) and Agilent Bioanalyzer 2100 System. At last, the libraries were sequenced using 2  250 bp paired-end sequencing on the HiSeq PE250 platform.