In vitro selection for Microprocessor cleavage: partial-randomization in apical regions
Cleavage products (pre-miRNA segments) were gel purified, reverse transcribed and amplified by for sequencing.  Libraries for four miRNA selections were mixed together before sequencing.  For each pri-miRNA, a reference pool and 1-2 selected pools (barcoded) are included.  Unique sequences that distinguish the pri-miRNAs and the individual pools are supplied below.  Indices are 0-based.    hsa-mir-125a selection:       Reference pool: TCCCTGAGACCCTTTAA, position 0; TGAGGTTCT, position 43       Selected pools: TCCCTGAGACCCTTTAA, position 2; TGAGGTT, position 45            Selected pool 1: GA, position 0            Selected pool 2: CT, position 0    hsa-mir-16-1 selection:       Reference pool: TAGCAGCACGTAAATATTG, position 0; GTATTAA, position 45       Selected pools: TAGCAGCACGTAAATATTG, position 2; GTATT, position 47            Selected pool 1: GA, position 0            Selected pool 2: CT, position 0    hsa-mir-30a selection:       Reference pool: TGTAAACATCCTCGACTGG, position 0; TCAGTCGG, position 44       Selected pools: TGTAAACATCCTCGACTGG, position 2; GTATT, position 46            Selected pool 1: GA, position 0            Selected pool 2: CT, position 0    hsa-mir-223 selection:       Reference pool: CGTGTATTTGACAAGCTGAGT, position 0; AGTTTG, position 45       Selected pools: CGTGTATTTGACAAGCTGAGT, position 2; AGTT, position 48            Selected pool 1: GA, position 0            Selected pool 2: CT, position 0