phoretic_foreleg_varroa_destructor_rep3
Total RNA was extracted as described before (a pool of approximately 50 individuals, in three replicates, in total 150 mites per group) (Singh et al., 2016).  Legs were dipped in liquid nitrogen, ground and extracted for total RNA using Geneall Kit (Seoul, Korea) following the manufacturers protocol. The quality and quantity of the RNA were measured using a NanoDrop 2000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA), and Bioanalyzer (Agilent 2100).