GSM4567532: Patient 24 week0; Homo sapiens; RNA-Seq
Whole blood samples were obtained at the start of the therapy with abatacept and at week 12. Blood was collected using RNA-stabilizing PaxGene tubes (PreAnalytiX, Switzerland), which preserve total RNA from the time of venipuncture. Total RNA was extracted using the PaxGene blood isolation kit (Qiagen). All samples had a RIN index > 7 and were included for RNA-seq analysis RNA-seq libraries were performed using with the KAPA RNA HyperPrep Kit, with RiboErase (HMR) Globin (globin and rRNA depletion protocol) for Illumina sequencing platforms and with the addition of Unique Molecule Identifiers (UMIs). Sequencing was performed using the NovaSeq platform (Illumina) at average of 250 paired-end reads
GEO Accession
GSM4567532