Illumina HiSeq 2500 paired end sequencing; ChIP-Seq (H3K4me3, H3K27me3) of steady-state splenic classical dendritic cells from wild type, germ-free and IFNAR knockout mice
ChIP-Seq (H3K4me3, H3K27me3) of steady-state splenic classical dendritic cells from wild type, germ-free and IFNAR knockout mice
Splenocytes were isolated and sorted for CD3 CD19 CD45 CD11c MHC-II to high purity. Fixed chromatin from isolated cells was sheared and precipitated for H3K4me3 and H3K27me3 Libraries were prepared by the Deep Sequencing Unit at the MPI in Freiburg, Germany
Experimental Factor: C57BL/6
strain
Experimental Factor: wild type genotype
genotype
Experimental Factor: H3K4me3
immunoprecipitate