GSM1533409: MCF7_E2_80min_Pol_II; Homo sapiens; ChIP-Seq
For DNA-Seq: lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. For RNA-Seq: total RNA was isolated using TRIzol according to the manufacturer's recommendations, followed by DNaseI treatment. 100–250 ng total RNA was subjected rRNA depletion with the Ribo-Zero Magnetic Gold Kit and processed further for strand specific RNAseq. DNA and RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM1533409