16S V4 of adult central oropharyngeal microbiome
16S V4 amplicon sequencing using Illumina MiSeq (250bp PE) was performed as described in Development of a dual-index sequencing strategy and curation pipeline for analyzing amplicon sequence data on the MiSeq Illumina sequencing platform by Kozich et al. at MSU RTSF Genomics Core (rtsf.natsci.msu.edu)