16S rRNA sequencing of trap-collected feces and potential sources of microbial contamination
"We conducted high throughput sequencing of the 16S rRNA gene from woodrat feces collected aseptically, and from trap contents after animals were in traps overnight. We also inventoried microbes from potential sources of microbial contamination (bait, trap surface, cotton bedding). DNA was extracted using a Qiagen Stool mini kit. PCR and sequencing was conducted by Argonne National Lab."