GSM3391901: Parthenogenetic ESC-7 (Rep.3); Sus scrofa; RNA-Seq
Before RNA extraction, SSEA1-positive undifferentiated pig ESCs were sorted using a MACS Cell Separation System (Miltenyi Biotec, Germany) to remove differentiated cells and feeder cells. Total RNA was extracted from pig fetal fibroblasts and sorted SSEA1-positive ESCs using TRIzol reagent (Invitrogen). The quality of RNA samples was examined using the Agilent 2100 BioAnalyzer (Agilent, Santa Clara, CA, USA) (Table S5). The extracted RNA samples were used for construction of cDNA libraries. The cDNA library preparation and RNA-Seq analysis were performed by Theragen Etex (Korea). The libraries were prepared for 100 bp paired-end sequencing using the TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA, USA). The quality of the cDNA libraries was evaluated using the Agilent 2100 BioAnalyzer (Agilent). The cDNA libraries were quantified using the KAPA Library Quantification Kit (Kapa Biosystems, Wilmington, MA, USA) according to the manufacturer's instructions. Fibroblasts were isolated from fetus at dpc 30, and RNAs were extracted from fibroblasts at passage 6 in the same manner as the pig ESCs.
GEO Accession
GSM3391901