GSM3861984: miR290 SE untargeted; Mus musculus; Bisulfite-Seq
For miR290 SE Nanog+RGM+ DKO and miR290-SE Nanog+RGM+, cells were preplated and sorted based on Nanog-eGFP and RGM-tdTomato signals. For miR290 SE untargeted, cells were preplated. PCR amplicons were sonicated using Covarius into 150-200bp range. NEBNext® Ultra™ DNA Library Prep Kit for Illumina and NEBNext® Multiplex Oligos for Illumina® were used to construct libraries according to manufacturer's protocol. Single barcoded library was prepared from sonicated bisulfite PCR amplicon fragments of the miR290 SE wildtype-allele using NEBNext® Ultra™ DNA Library Prep Kit for Illumina (NEB #E7370S) and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1, NEB #E7335S).
GEO Accession
GSM3861984