Metatranscriptome of deep biosphere: modern marine aquifer
Samples were collected with RNA sampling device with an in-built fixation system. RNA was extracted from all filters using the MO BIO PowerWater RNA isolation kit. Library preparation was carried out using the Illumina HiSeq TruSeq Nano DNA Library Prep Kit for NeoPrep. Clustering was done by 'cBot'. Sequencing was performed using 'HiSeq SBS Kit v4' chemistry. The Bcl to FastQ conversion was performed using bcl2fastq-1.8.4 from the CASAVA software suite.