GSM3938639: T47D cells, siCT T6H replicate 1; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol (Thermo Scientific) and 3 biological replicates for each condition were performed. cDNA libraries were prepared using the SENSE mRNA-Seq Library Prep Kit V2 (Lexogen, Vienna Austria). Quality of the cDNA was assessed and RT-qPCR was performed for selected PR target genes as quality control. Standard illumina protocols were used to construct the library for sequencing.