GSM1904085: sperm wt rep1; Mus musculus; ncRNA-Seq
The total RNA was isolated using the mirVana miRNA Isolation Kit (Life Technologies) following the manufacturer’s instructions with modifications at the lysis stage. In brief, after addition of lysis buffer, the frozen sperm pellets were homogenized at low settings for 90 seconds, followed by a five minute incubation at 65 °C.  Complete lysis of sperm heads was verified by microscopic examination.  Once a total lysis of sperm heads was achieved, the samples were then placed on ice, and the default protocol was resumed.  Samples were then placed on ice, and the default protocol was resumed. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM1904085