GSM4193081: RNA extracted from batch 1 of embryos transfected with epiB::ciHMX and epiB:YFP; Ciona intestinalis; RNA-Seq
Fluorescent transfected embryos were collected in 1.5ml tubes. Embryos were dissociated in TRIZOL, dissociating via gentling passing through a needle. RNA was subsequently extracted using phase separation with chlorofom, pricipitated with isopropanol, followed by ethanol washes and re-suspension in nuclease free water. The mRNA fraction was selected from the total RNA provided before conversion to cDNA. Second strand cDNA  synthesis incorporated dUTP. The cDNA was end-repaired, A-tailed and adapter-ligated. Prior to amplification,  samples underwent uridine digestion. The prepared libraries were size selected, multiplexed and QC'ed before paired  end sequencing was carried out over four lanes.
GEO Accession
GSM4193081