 whole genome sequence data for SardiNIA individual 13155 
DNA is quantitated using pico green assay.  3-5 ug DNA is sheared to 400 bp using Covaris sonication.  Libraries are generated using sample prep kits from Illumina and New England BioLabs.  The DNA is end repaired, purified, poly-A tailed, purified again and indexed adapters are ligated using T4 DNA Ligase reagent.  The libraries are then size selected to 450 bp by gel electrophoresis and amplified with 10 cycles of PCR.  Libraries are QC'd using the Agilent Bioanalyzer.  Equimolar pools are made and quantified using qPCR (Kapa Biosystems kit).  Flow cells are clustered using the Illumina cBot and sequenced on Illumina HiSeq 2000.