GSM3939021: E-DiTD11; Homo sapiens; RNA-Seq
Total cellular RNA was extracted from 15-20 randomly collected intact and re-aggregated organoids, and from a 9 cm2 well for the monolayer culture at TD 2, TD 11 and TD 31 and purified using PicoPure RNA isolation kit (Applied Biosciences). Poly(A)+ RNA was used as template for the preparation of cDNA libraries, which were then subject to single end RNA sequencing experiments. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM3939021