Genomic SMRT sequencing of X. oryzae pv. leersiae NJ611
Long read, single molecule real time sequencing (SMRT, PacBio, Menlo Park, CA) data was generated for five Xol strains and for the Xo strain X11-5A, to be used as an outgroup. DNA for SMRT sequencing was isolated by culturing strains on nutrient agar for 48 h then using the Genomic DNA buffer set and Genomic-tips according manufacturer instructions (Qiagen, Valencia, CA). SMRT sequence was assembled using HGAP v4 (PacBio, Menlo Park, CA). Genomes were circularized using circulator (Hunt et al., 2015).