GSM2339909: mESC_DKOzero_WGBS; Mus musculus; Bisulfite-Seq
100-200 ng of genomic DNA was fragmented using a Covaris S2 for 6 min according to the following program: duty cycle 5%; intensity 10; cycle per burst 200. The sheared DNA was purified using the DNA Clean & Concentrator kit from Zymo Research. Bisulfite conversion of DNA was then conducted using the EZ DNA Methylation-Gold kit (Zymo Research), eluting in 15 µl low TE buffer. To minimize loss during storage, bisulfite-converted DNA was immediately processed for generating WGBS libraries using the Accel-NGS Methyl-Seq DNA library kit (Swift Biosciences). The libraries were sequenced for 100-bp paired-end reads on an Illumina HiSeq 2500 sequencer. genomic DNA was purified using Phenol/Chloroform extraction
GEO Accession
GSM2339909