GSM3938697: 293FlpIN_pcDNA5_Xrn2_WT_Dox_Trip_5_PolII_Rep2; Homo sapiens; ChIP-Seq
Cross-linked chromatin was sonicated with a Bioruptor or Covaris Ultrasonicator 1-2 mg of cross-linked extract was immunoprecipitated, and prepared for illumina library construction by repair with Klenow, T4 DNA polymerase and T4 polynucleotide kinase, A-tailing with Klenow exo-, ligation to branched adaptors, SPRI bead purification of ~200bp fragments, and amplification (15-18 cycles) with Illumina Tru-seq primers using Phusion DNA polymerase.
GEO Accession
GSM3938697