Illumina HiSeq 2000 paired end sequencing; Changes in the coding/non-coding transcriptome and DNA methylome that define the Schwann cell repair phenotype after nerve injury
Changes in the coding/non-coding transcriptome and DNA methylome that define the Schwann cell repair phenotype after nerve injury
No injury was performed on control samples. Sciatic nerves of adult mice were harvested at the sinotic notch. DNA was isolated from samples using phenol chloroform method.  Libraries were prepared without pooling to generate methylation profiles on the HiSeq 2000 platform to generate 100bp pair end reads as described in (Johnson et al 2012: DOI: 10.1002/0471142727.mb2123s99)
Experimental Factor: methylation profiling by high throughput sequencing
protocol
Experimental Factor: none
injury