GSM4680699: WT_Fibro_2; Mus musculus; RNA-Seq
Small intestines from adult, male C57Bl/6 mice were harvested and digested with Liberase TL. Single, live, CD45neg, EpCAMneg, CD31neg, gp38+ cells were either sorted into 384-well plates (Smartseq2) or captured in an oil emulsion (10X Genomics). RNA was amplified, barcoded and sequenced. Smartseq2: 0.0025 μl of a 1:40,000 diluted ERCC spike-in concentration stock was used, and all cDNA was amplified with 22 PCR cycles before QC control with a Bioanalyzer (Agilent Biosystems). The libraries were sequenced on a HiSeq2500 (Illumina) with single 50-bp reads (dual indexing reads). 10X Genomics libraries were prepared according to manufacturer's protocols and libraries were sequenced on a HiSeq4000 (Illumina). scRNA-seq
GEO Accession
GSM4680699