GSM4434536: WT_small RNA; Zea mays; miRNA-Seq
For small RNA-seq, total RNA was extracted using TRIzol reagent (Invitrogen) followed by treatment with DNase I (Promega). For bulk RNA-seq, RNA was extracted with the PicoPure RNA isolation kit (Arcturus), treated with DNaseI (Qiagen) and linearly amplified with the TargetAmp 2-Round aRNA Amplification kit 2.0 (Epicentre Biotechnologies). sRNA-Seq libries were prepared from 1.2 𝝁g total RNA using TruSeq small RNA sample preparation kit (Illumina). Libraries were quantified with the KAPA Illumina library Quantification Kit (KAPABIOSYSTEMS) and sequenced on the Illumina HiSeq2000 platform; RNA-Seq Libraries were constructed from linearly amplified RNA using the TruSeq RNA Library Kit from Illumina following the recommended protocols.
GEO Accession
GSM4434536