Tn-seq of GBS after blood outgrowth: subject 2 rep 2
We performed GBS mutant library outgrowth for six hours in five samples of fresh whole blood from three healthy adult volunteers and one control condition of selective media. Then bacterial genomic DNA was purified from each sample, digested with MmeI, ligated to barcoded adapters, and used as template for PCR. The resultant amplicons were purified and sequenced by NGS.