16S rRNA gene-targeted sequence of microbial community DNA within the gut of a Nigerian woman
The primers 341F and 805R (with added Illumina adaptor sequences) were used for the targeted amplification of the V3-V4 region of the 16S rRNA gene as described previously (Candela et al. 2016). The magnetic bead-based clean-up system (Agencourt AMPure XP; Beckman Coulter, Brea, CA, USA) was used to purify the amplicons. The Nextera technology was used to prepare indexed libraries by limited-cycle PCR.