RNA-seq using brains of fruit fly for investigating lncRNAs in fruit fly- RiboZero
Total RNA was purified from four-day post-eclosion wild-type male brains by NucleoSpin® RNA II purification kit. RNA-seq was performed by using strand-specific library with Ribo-Zero™ Gold Kit to generate paired-end 90-bp reads on the Illumina Hi-seq 2000 platform.