GSM2758642: RNA-Seq_total_nuclear_r2; Homo sapiens; RNA-Seq
Nuclei were isolated from crosslinked cells by scraping, resuspending, washing once with cold PBS and resuspending in 9 mL of cold PBS, 27 mL of cold ddH2O and 9 mL of 5x Nuclear isolation buffer (1.28 M sucrose, 40 mM Tris pH 7.5 at 4 °C, 20 mM MgCl2, 4% Triton X100, 1 µg/mL each for Leupeptin, Pepstatin A, Aprotinin). After 15 minutes rotating at 4 °C nuclei were pelleted at 1800 rcf for 10 minutes at 4°C. Nuclei were washed once in the same buffer then resusended in Trizol. RiboZero Gold, TruSeq Stranded TotalRNA
GEO Accession
GSM2758642