Amblyomma americanum  Illuminasequence reads
Ticks were stored in 70% ethanol at 4C until their DNA was extracted. Ticks were then surface disinfected with sequential washes of 10% bleach and 70% ethanol, rinsed 3 times in sterile distilled water, and frozen in a close-fitting Eppendorf 1.5 ml microcentrifuge tube by immersion of the lower portion of the tube in liquid nitrogen. Homogenization of single or bilaterally bisected adult ticks to powder was accomplished with a Kontes pestle prior to DNA extraction with the Promega (Madison, WI, USA) Wizard SV 96 Genomic DNA Purification System. Purified tick DNA in 200 ml AE buffer was stored at 4oC until library preparation. Purified tick DNA was quantified with the Qubit HS-DNA assay (Life Technologies).  The DNA of the single adult female was submitted to the Centers for Disease Control and Prevention Biotechnology Core Facility and a TruSeq Sample Preparation Kit (Illumina, Inc., San Diego, California) was used to prepare a 100 bp paired-end shotgun library with a target insert size of 300 bp. The library was sequenced on an Illumina HiSeq2500 using a HiSeq SBS kit.