In vitro bile inoculation 24 hr replicate 2
Total RNA isolation was performed using the miRNeasy Mini Kit (QIAGEN) , A total of 2.5 µg of confirmed DNA-free total RNA was treated with the Ribo-Zero Magnetic Gold rRNA Removal Kit (Epidemiology) according to the manufacturerÕs instructions (Illumina).Library prep was made with TruSeq stranded mRNA HT library preparation kit (Illumina) with some modifications