Whole genome sequencing of GaHV-2 (MDV) derived from a hyperdiverse population established by polymerase mutant Y567F in cell culture passage 16: Clone 11
Viruses were obtained from infected animal tissues and propagated for 2-3 passages in Chicken Embryonic Cell culture. Libraries created from single virus genomes, clonally selected as BACs in E.coli, standard library prep (NEB Next Ultra II) from 1 ug BAC DNA, 3 PCR cycles to complete adapters/indices.