GSM2584808: J-34 2i/L to S/L p23; Mus musculus; Bisulfite-Seq
RRBS libraries were prepared using a commercial kit (Ovation RRBS Methyl-Seq System, NuGen, San Carlos, CA) following the manufacturer's protocol except that we pooled 12 individually barcoded reactions after the final repair step and performed the bisulfite conversion and library amplification as a pool. Libraries were sequenced on an Illumina HiSeq2500 high-output flowcell without a PhiX spike-in using Nugen's custom sequencing primer and standard Illumina sequencing primers to read the sample barcodes
GEO Accession
GSM2584808