GSM2582392: ChIPseq for H3K9me3 in wt mES cells, replicate 1; Mus musculus; ChIP-Seq
10^7 mES cells were crosslinked, chromatin was extracted and fragmented with micrococcal nuclease to mostly mono nucleosomes. 1% of chromatin was set aside as Input control. The chromatin was incubated with antibody over night, Protein A coupled Dynabeads were added for 2 hours and thereafter immunoprecipitated DNA was washed and de-crosslinked. Purified DNA was subsequently used for library preparation. For a detailed protocol, see Ostapcuk et al, 2017. Libraries were prepared using the NEB Next DNA library prep kit according to manufacturer's instructions. For barcodes, see sample characteristics.
GEO Accession
GSM2582392