GSM3557974: BCSCs_MCF7; Homo sapiens; RNA-Seq
Cellular suspensions were loaded on a Single-cell Instrument (10x Genomics, Pleasanton, CA) to generate single cell GEMs. Single cell was reverse-transcribed using the Discover-scTM WTA Kit V2 (Vazyme, N711) and incorporated into the cDNA library using TruePrep DNA Library Prep Kit V2 for Illumina® (Vazyme, TD503). Sequencing libraries were loaded on an Illumina Hiseq PE150 a 150 bp paired-end module. The raw reads were filtered by removing reads containing adapter, ploy-N and low-quality reads for subsequent analysis. The Cell Ranger Single Cell Software Suite 1.3 was used to perform sample demultiplexing, barcode processing and UMI counting. All barcodes whose total UMI counts exceed m/10 are called as cells.
GEO Accession
GSM3557974