GSM1533188: LSD1_ChIPSeq; Rattus norvegicus; ChIP-Seq
Histone-DNA complexes of lysates from sonicated nuclei were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the Paired-End DNA Sample Prep kit. Briefly, DNA was end-repaired using a combination of T4 DNA polymerase and Klenow polymerase. A single ‘A’ nucleotide is added to the 3’ ends of the blunt fragments through being treated with Klenow fragment, and then the 3-'A' ended DNA was ligated to Illumina's adapters which have a single 3-'T' base using DNA ligase. After adapter ligation DNA was PCR amplified with primers and library fragments of 100-300 bp (insert plus adaptor and PCR primer sequences) were selected. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols.
GEO Accession
GSM1533188