RNA-Seq of mus musculus: adult male lung
Ribosome RNAs were removed from total RNA.RNA samples were fragmented to approximately 200 bp. Purified RNAs were reversely transcribed to the first cDNA strands. the second cDNA strands were synthesized The purified library productswere evaluated and diluted to 10 pM for the generation of DNA clusters. The paired-end flow cells were sequenced on HiSeq. 2500.