GSM2584462: Original_Cross_ rep1; Mus musculus; ncRNA-Seq
Trizol was used to extract RNA from the sperm samples with slight modificatiosn retain small RNAs. Sperm from each mouse was processed separately and treated as a replicate. Two biological replicates per condition were produced. For each sample, 250-1500ng of RNA were used for size-selection on a polyacrylamide gel. After elution from the gel we proceeded to library preparation. The Truseq small RNA library preparation kit from Illumina was used for library production according to Manufacturer's instructions. Samples (4 in total) were barcoded (Sample 1 GATCAG, Sample 2 TAGCTT, Sample 3 GGCTAC, Sample4 CTTGTA) and run in the same sequencing lane.
GEO Accession
GSM2584462