GSM4434564: KP tumor d35 Ams #3; Mus musculus; RNA-Seq
Sorted TRMs in the lung of naïve and KP-tumor bearing mice at 2 different time points (d15 and d35 post-tumor transplantation). For library preparation: RNA isolation from 20000 FACS-sorted TRM was done with the Qiagen RNeasy micro kit. 1ng of RNA was synthesized into cDNA using Smart-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Bio). Sequencing libraries were prepared using the Low Input Library Prep Kit (Takara Bio). Libraries were sequenced on an Illumina NextSeq 550 System.
GEO Accession
GSM4434564