16S of incubated soil: Arctic permafrost and active layer
DNA extractions were performed using MoBios PowerSoil-htp 96 Well Soil DNA Isolation Kit as per protocol, the V4 region of the 16S rRNA gene was amplified using PCR primers 515F/806R, DNA quantification was performed using PicoGreen, and 2x150bp paired-end sequencing was performed using an Illumina Mi-Seq instrument