GSM2418343: ATAC DN2 ThymoDp(A)/p(A) 2; Mus musculus; ATAC-seq
Harvested cells were stained with lineage marker, CD25, CD117, ghost dye red 780. Lineage negative DN2 cells were sorted by FACS AriaII and washed with PBS. 50000 cells were used for library preparation. Washed cells were resuspended in lysis buffer. After washing, cells were treated with transposition mix for 30 min at 37 degrees. Cycle of PCR amplification was determined by q-PCR. 100-800 bps of Libraries were selected from 2% agarose gel.
GEO Accession
GSM2418343