GSM1531547: Input_DNA_Control; Homo sapiens; ChIP-Seq
3 million cells crosslinked in 1% formaldehyde were lysed with Farnham and RIPA cold lysis buffers. Following collection, the crude nuclear preparation was processed in a Bioruptor at the high setting for a total of 15 minutes, in cycles of 30 seconds on/30 seconds off. The chromatin was collected by centrifugation and incubated with 5 µg of RNAPII-Ser5(P) antibody overnight The immunoprecipitated samples were end-filled using a combination of  T4 DNA polymerase and T4  polynucleotide kinase , 3’ -terminal-A extended with Klenow 5'-3' exo minus, and ligated to pre-annealed TruSeq indexed Illumina adapters. Libraries were amplified using Illumina primers and gel extracted for size selection and primer-dimer removal.  Before sequencing, libraries were tested using the BioAnalyzer to test quality in terms of size and primer-dimer depletion.
GEO Accession
GSM1531547