GSM2759559: GM12878 Aiolos Knockdown Replicate 2; Homo sapiens; RNA-Seq
Total RNA was purified with the RNeasy Mini Kit (QIAGEN). Quality was validated with the Agilent 2100 Bioanalyzer (Agilent RNA 6000 Pico Kit) and quantified with the Qubit RNA HS Kit. rRNA was depleted with the Ribo-Zero Gold rRNA Removal Kit from Illumina and depleted RNA purified using Agencourt RNAClean XP beads. Libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina. Sizing and analysis of fragments was assessed on the Agilent 2100 Bioanalyzer and Agilent High Sensitivity DNA Kit. Libraries were sequenced on the Illumina HiSeq 2500 platform with paired-end 120bp reads (Illumina).
GEO Accession
GSM2759559