FOXK1 ChIP-seq in ASXL1/2/3 KO
ChIP assays were performed  according to the standard protocols. Chromatin were crosslinked in 1%  formaldehyde (Sigma Aldrich) directly in the dish, and DNA was sonicated to  200 to 400 bp fragments by sonication (Bioruptor, Diagenode). 50 to 1000 ug  of chromatin of mESCs (with spike-in Drosophila chromatin) were incubated  with the various antibodies. ChIP-seq libraries were prepared according to  the standard protocols (NEB and Illumina)