GSM1631708: CD34_DMSO; Homo sapiens; RNA-Seq
Treated CD34+ and U937 cells were washed with PBS, then total RNA was extracted with the RNeasy mini plus kit from Qiagen. Total RNA was depleted of ribosomal RNA with the RiboZero gold kit (Epicentre). Enriched mRNA was then applied to library preparation according to manufacturer’s protocol (NEBNext Ultra). After repeated quality control for average DNA input size of 300 bp, the samples were sequenced on a HiSeq Illumina sequencer with 2x100bp paired-end reads.
GEO Accession
GSM1631708