HS250G8_SINGLE_DNA-Genomic DNA sequence of the single colony isolated from the  population of del hns-stpA evolved for 250 hour under laboratory condition
We generated the double mutant of hns and stpA (?hns-stpA) in                 Escherichia coli K12-MG1655 using ?-red recombinase method (Datsenko and Wanner).                 The double mutant was evolved under laboratory condition at 37°C in LB . Evolved                 population collected at different time points such as ~ 100 hours and ~250 hours                 were collected and frozen. Single colonies isolated from these population (16                 colonies from evolved population of 100 hour , 4 colonies from the population                 evolved for 250 hour and 4 colonies from the ?hns-stpA parent population ) were                 picked and genomic DNA was extracted using Sigma GenElute™ Bacterial Genomic DNA Kit                 (Cat.No. NA2120) using manufacturers instruction. Genomic DNA samples were prepared                 for sequencing by using the Illumina ® TruSeqTM DNA Sample Preparation Kit. Evolved                 genomes were sequenced for 100 cycles from both end in Illumina Hiseq1000                 platform