In vitro selection for Microprocessor binding: partial-randomization in basal regions
Bound pri-miRNA variants were recovered by nitrocellulose filtration, reverse transcribed, and amplified for sequencing.  Libraries for a reference pool and three rounds of selection on hsa-mir-125a were combined into a single sequencing run.  Unique sequences that distinguish the hsa-mir-125a sequence and the individual pools are supplied below.  Indices are 0-based.      hsa-mir-125a selection: GAGTCAG, position 5 (end 1)       Reference pool: CAT, position 0 (end 2)       Selected, Round 1: ATG, position 0 (end 2)       Selected, Round 2: TGA, position 0 (end 2)       Selected, Round 3: TAG, position 0 (end 2)