RNA-seq of malaria liver hypnozoites
Total RNA samples extracted from FACS-sorted small parasite infected cells (e.g. hypnozoites) and FACS-sorted large parasite infected cells (e.g. liver schizonts) were amplified using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech). The obtained cDNA samples were then shared to 200-500 bp lengh using a Covaris E220 instrument (Covaris) and subsequently processed with the Low Input Library Prep Kit v2 (Clontech) to generate sequencing libraries. RNA-seq cDNA libraries were then sequenced in paired-end mode, 2x76 bp, using the Illumina HiSeq2500 platform.