RNA-seq of Arabidopsis thaliana leaves infiltrated with Pseudomonas syringae DC3000 D28E carrying the effector HopAM1 or an empty vector
Leaves from five-week-old plants were infiltrated with thePseudomonas syringae strain DC3000 D28E carrying the effector HopAM1 or an empty vector. Samples were harvested immediatly before (T = 0h) and 2, 4, 6, 8, 10 and 12h after infiltration. Illumina mRNA-seq libraries were prepared from 1000ng total RNA and sequenced on a HiSeq2500 instrument at the High-Throughput Sequencing Facility at the University of North Carolina at Chapel Hill.