Variations on a theme: Genomics of sex determination in the cichlid fish Astatotilapia burtoni
DNA of each individual was extracted from caudal fin tissue with a Qiagen QIAamp DNA mini kit and quantified using a Qubit Fluorometer. RAD-sequencing library preparation was done at the University of Oregon, Eugene, following the method described in Amores et al 2011 Genetics 188(4)799-808. DNA of each individual  (500 ng in 50 μl) was digested with SbfI, labeled with a 5-nucleotide barcode adapter ligated to each restriction site during library preparation. Libraries were sequenced on an Illumina HiSeq 2500 using the 100bp-single-end mode