GSM2579644: HCT116.ATAC-seq; Homo sapiens; ATAC-seq
For NicE-seq and ATAC-seq, genomic DNA was extracted using phenol chloroform method. For ATAC-seq, 25000 HCT116 cells were processed according to previously published protocol (Buenrostro et. al, doi:10.1038/nmeth.2688) with the following modifications. After addition of lysis buffer the cell suspension was passed through a 27 G ½ needle six times to facilitate cell lysis. ATAC-seq library was size-selected for 250-500 bp and 75 bp paired-end sequenced on NextSeq 500 Illumina platform.
GEO Accession
GSM2579644