Quiescent RPE-1 cells, pre-treated  with 10 µM ATM inhibitor (KU55933 ) and  irradiated with 20 J/cm^2 UV-C.
Human retina pigmented epithelium cells (RPE1) were grown to confluence and serum deprived for 72 hours to ensure quiescence. Cells were treated for 1hour with 0.01mM ATM inhibitor KU55933 and subsequently UV irradiated with 20J/m^2 UV-C (245nm). RNA was isolated 6hours post irradiation with the RNeasy kit (Qiagen) and PolyA RNA was isolated using the Dynabeads mRNA purification kit (Invitrogen). Sample integrity was verified with the Agilent 2100 Bioanalyzer. For each sample, cDNA library was prepared and validated using the Illumina TruSeq RNA sample preparation kit v2. Equal amounts of polyA RNA were chemically fragmented to approximately 350 nt. RNA-seq was performed according to the Illumina TruSeq v3 protocol on the HiSeq 2500 platform resulting in 100bp paired end reads.