Tetranychus cinnabarinus
The poly-(A) mRNA was enriched and isolated from the total RNA samples using Magnetic Oligo(dT) Beads, followed by fragmentation of mRNA with the RNA fragmentation kit (AMbion, Austin, TX ). Reverse transcription into cDNA was performed using random hexamers and reverse transcriptase (Promega, Madison, USA), and then second strand cDNA was synthesized using DNA polymerase I  (Invitrogen, Beijing, China) and RNaseH (New England Biolabs, Ipswich, MA). After purified by QIAquick PCR Purification Kit, cDNA fragments were end-repaired, and were then ligated to solexa adaptor after adding a single “A” at their 3’ end. The desired size of adaptor ligated fragments was selected by agarose gel electrophoresis. PCR was performed to selectively enrich and amplify the selected cDNA fragments to construct the cDNA libraries for paired-end sequencing using the illumine Hiseq 2000 (Biomarker Technologies Co., Ltd, Beijing, China).