GSM3099429: NS_B; Homo sapiens; RNA-Seq
Cells were lysed in QIAzol Lysis Reagent (Qiagen), scraped off the wells and briefly vortexed. RNA was isolated using the RNeasy Mini Kit (Qiagen) following the manufacturer's instruction and RNA integrity was assessed (Bioananalyzer 2100, Agilent). RNA was diluted to 10 ng/μl and converted to mRNA libraries using the TruSeq Stranded mRNA kit (Illumina) with dual indexing following standard instructions but with 5 min fragmentation time. Libraries were validated on the Fragment Analyzer platform (AATI) using a standard sensitivity NGS fragment analysis kit, and concentrations were determined using the Quant-iT dsDNA High Sensitivity assay kit on the Qubit fluorometer (Thermo Fisher Scientific). Sample libraries were pooled in equimolar concentrations, diluted and denatured according to Illumina guidelines.
GEO Accession
GSM3099429