RRBS Gasterosteus aculeatus: muscle tissue, adult, warm acclimated
genomic DNA isolated from threespine stickleback muscle tissue was digested with the methylation sensitive restriction enzyme MspI. MspI digested gDNA served as the input material for the Bio-O NEXTflex Bisulfite-Seq Kit, with the following steps: End Repair, Adenylation, Adapter Ligation (NEXTflex Bisulfite-Seq Barcodes), Gel-Free Size Selection (Agencourt AMPure XP Beads). The purified adapter ligated DNA was then subjected to Bisulfite Conversion (Zymo Research EZ DNA Methylation Gold Kit)