GSM4680368: TG_5-transgene-am-WGBS_TAG; Mus musculus; Bisulfite-Seq
DNA and RNA were isolated using TRIzol reagent (Sigma Aldrich, Darmstadt, Germany) and further AllPrep DNA/RNA Mini Kit (Qiagen, Hilden, Germany) purification, following the manufacturer's instructions. For RNA isolation (RNase-free DNAse set, Qiagen) DNAse I digestion was performed on the column. Proteinase K digestion was applied for RNA and DNA isolation (Puregene Proteinase K, Qiagen). Prior to library preparation, DNA and RNA quality was checked by Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) and quantified by Qubit fluorimetry (Thermo Scientific, Wilmington, USA). All RNA samples reached an RNA integrity number (RIN) > 8.5. Tagmentation based WGBS followed the protocol described in (Peat & Smallwood, 2018) with 20 ng genomic DNA as input. From each tagmentation reaction, four sequencing libraries with different barcodes were generated, and further pooled in equimolar amounts with a final concentration of 10 nM. Library pools were sequenced at the DKFZ Genomics and Proteomics Core Facility using paired-end, 125 bp, on one lane of a HiSeq2000 v4 sequencer (Illumina).
GEO Accession
GSM4680368