GSM1533529: Col0 replicate 3 small RNA; Arabidopsis thaliana; ncRNA-Seq
Total RNA was isolated with TRIzol (Ambion) from 0.1 g flowers. High-molecular-weight RNA was precipitated with 12.5% PEG8000 and 0.5 M NaCl. Low-molecular-weight RNA from the supernatant was ethanol-precipitated and 15-40-nt RNAs were excised from denaturing polyacrylamide/Urea gels and purified in final 6 µl water. Individual TruSeq small RNA libraries from three biological replicates each were generated according to the manufacturer’s instructions (Illumina), except that 13 cycles of PCR amplification were used.
GEO Accession
GSM1533529