Resequenced Genome Reads for Bostrychus sinensis sample WZ26
Two hundred mg of muscle tissue was cut into pieces with a knife and transferred to a preheated (56) 1.5 ml tube with 0.6 ml lysis buffer containing 2 mg proteinase K.  The tube was incubated at 56 for 1-2 hours, then was centrifuged at 12,000 rpm at room temperature for 10 minutes.  The supernatant was extracted vigorously with one volume of phenol/ chloroform/isopentanol (25:24:1) followed by centrifugation at 18,213 g at room temperature for 10 minutes in a new tube.  The supernatant was transferred into another new tube with a 2/3 volume of isopropanol and 100 l sodium acetate trihydrate followed by gently mixing.  The mix was put at -20 for more than two hours and centrifuged at 12,000 rpm for 20 minutes at room temperature.  The pellet was then washed in 1 ml of 75% ethanol.  The ethanol was removed by centrifuging and air drying the pellet for several minutes.  The pellet was dissolved and stored in 30-200 l TE for further study. After DNA extraction, 1 g of genomic DNA was randomly fragmented by the Adaptive Focused Acoustics method (Covaris, Beijing, China) followed by purification with the Axygen AxyPrep Mag PCR Clean-up Kit (Thermo Fisher Scientific, Shanghai, China).  The fragments were end repaired with the Fast DNA End Repair Kit and purified.  The repaired DNAs were combined with A-Tailing Mix, then the Illumina adapters were ligated to the adenylate 3ends followed by product purification.  The products were selected based on insert size.  Several rounds of PCR amplification with the PCR Primer Cocktail and PCR Master Mix were performed to enrich the adapter-ligated DNA fragments.  After purification, the library was qualified with the Agilent Technologies 2100 Bioanalyzer and ABI StepOnePlus Real-Time PCR System.  The qualified libraries were sequenced using the Illumina Hiseq X Ten System with PE 150-bp reads.