16S rRNA amplicon sequencing of human feces from healthy and IBS cohorts for microbial community profiling
Genomic DNA was amplified using primers 341F and 805R. Nextera barcodes were attached to forward reads and purity was verified via agarose gel. Products were then purified with a Qiaquick PCR purification kit. Short fragments were removed and the remaining  samples were pooled and then sequenced.