RAL-375 in stress conditions for ATAC-seq replicate 2
The protocol used for ATAC-seq was adapted from Buenrostro and colleagues (PMC4374986) for working with Drosophila rather than human cells. Thirty midguts from 4 to 6 days old females were isolated and immediately placed in 200 l ice cold PBS. They were lysed and homogenized to isolate between 50,000 and 100,000 cells using a Neubauer camera. Then, the remanining ATAC-seq protocol was performed following the aforementioned protocol. Sequencing was done in paired-end and 50nts long obtaining a minimum of 25 million pair reads per sample.