WGS of E. coli O26: Escherichia coli O26 isolate 1 collected from composite calf bedding material (woodchip) on 2-Sep-2016
High quality genomic DNA was extracted from each selected isolate using the QIAamp DNA Mini Kit (QIAGEN, Hilden, Germany) following the manufacturer guidelines. DNA paired-end libraries were prepared from a volume of 80 to 100 L of each eluted DNA (155 ng/L) using the Illumina Nextera XT DNA Library Preparation Kit, version 2 (Illumina, Inc.). The resulting libraries with 683 bp inset sizes were sequenced on the Illumina MiSeq Next Generation Sequencing platform (Illumina, Inc.) using version 3 chemistry