GSM4191352: POLR3A input in MDA-LM2 rep 2; Homo sapiens; ChIP-Seq
400 uL Lysis buffer (1% SDS, 50 mM Tris Hcl pH 8.0 20 mM EDTA, protease inhibitors (Roche)) and incubated on ice for 10 minutes. Lysates were then sonicated to produce DNA fragments between 200 – 1000 bp with settings Amplitude 70, 10 sec on 30 sec rest for three repetitions on Sonicator S-4000 (Branson) with Microtip and Ultrasonic Liquid Processor (Misonix) Samples were PCR amplified with Illumina barcodes to construct a multiplexed library. 50 SR HiSeq at the Rockefeller Genomics Resource Center
GEO Accession
GSM4191352