Sanger sequencing of bacterial pure cultures isolated from healthy corals in the GBR
Coral fragments were obtained from healthy coral colonies (Acropora millepora and Pocillopora damicornis) collected on the Great Barrier Reef and brought to the Australian Institute of Marine Science. After washing in filtered seawater (FSW), coral tissue slurries were produced by airbrushing each coral fragment into 5 mL of ASW and a dilution series was  inoculated onto modified minimal marine agar. After 2 days of incubation at 28C, single bacterial colonies were transferred into Difco Marine Broth and grown overnight in a shaking incubator at 28C. Liquid cultures were re-streaked onto minimal marine agar; the procedure was repeated until pure cultures were obtained. The 16S ribosomal RNA gene bas amplified with the primers 27F/1492R and sent to Macrogen Korea for Sanger sequencing.