Genomic DNA sequence of the single colony isolated from the  del hns-stpA double deletion mutant generated by lambda red recombinase system
We generated the double mutant of hns and stpA  (?hns-stpA) in Escherichia coli  K12-MG1655 using  ?-red recombinase method (Datsenko and Wanner). The double mutant was evolved under laboratory condition at 37°C in LB .  Evolved population collected at  different time points such as ~ 100 hours and ~250 hours were collected and frozen. Single colonies isolated from these population (16 colonies from evolved population of 100 hour , 4 colonies from the population evolved for 250 hour and 4 colonies from the ?hns-stpA parent population ) were picked and genomic DNA was extracted using Sigma GenElute™ Bacterial Genomic DNA Kit  (Cat.No. NA2120) using manufacturers instruction. Genomic DNA samples  were prepared for sequencing by using the  Illumina ® TruSeqTM DNA Sample Preparation Kit. Evolved genomes were sequenced for 100 cycles from both end in Illumina Hiseq1000 platform.