GSM4567698: B-231NC-2; Homo sapiens; RNA-Seq
Total RNA from MDA-MB-231 shNC/shBry was prepared and kept at 80°C. The RNA quality was determined using a Bioanalyzer 2200 (Agilent, SantaClara, CA, USA). RNA with RIN (RNA integrity number)>8.0 was considered acceptable for cDNA library construction. Sequencing and bioinformatic analysis were performed byShanghai Novelbio. Genes were considered to be significantly differentially expressed between groups when the P-value was<0.05 and the fold change of expression was more than 1.5 RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM4567698