Longitudinal RNA-Seq analysis of acute and chronic neurogenic skeletal muscle atrophy
Skeletal muscle was homogenized in TRIzol using RNase-free stainless steel beads.  Homogenates were centrifuged at 10,000 rpm at 4C for 10 min to pellet debris, and RNA was purified from the TRIzol supernatant using a Direct-Zol RNA mini purification kit with on-column DNase digestion. RNA-sequencing was carried out using TrueSeq RiboZero gold (stranded) kit. Libraries were indexed and sequenced over 18 lanes using HiSeq4000 (Illumina) with 69-bp paired end reads.