Metagenome sequencing of P03 control enriched Time 1C near bottom water
The libraries were prepared using Nextera DNA Sample preparation kit (Illumina) following the manufacturer's user guide. The initial concentration of DNA was evaluated using the Qubit dsDNA HS Assay Kit (Life Technologies). 50 ng DNA was used to prepare the libraries. The samples underwent the simultaneous fragmentation and addition of adapter sequences. These adapters are utilized during a limited-cycle (5 cycles) PCR in which unique indices were added to the samples. Following the library preparation, the final concentration of the library was measured using the Qubit dsDNA HS Assay Kit (Life Technologies), and the average library size was determined using the Agilent 2100 Bioanalyzer (Agilent Technologies).  The libraries were pooled and diluted (to 10.0pM) and sequenced paired end for 300 cycles using the HiSeq system (Illumina).