GSM3693263: H3K9me3_ChIPseq_Tex191ESC_KO3Input; Mus musculus; ChIP-Seq
Embryonic stem cells were detached from 70% confluent T75 flasks by scraping and resuspended in ice-cold PBS. Cross-linked ChIP was then performed essentially as previously described (Mortazavi et al., 2006, Genome Res. 16, 1208–1221) by fixing cells with formaldehyde, collecting nuclei from lysates, sonicating then isolating histone-DNA complexes with anti-histone H3K9me3 antibody (Abcam ab8898). Samples of input DNA were removed prior to the addition of anti-histone H3K9me3 antibodies. Libraries were prepared using an NEBNext Ultra ChIPseq kit and sequenced using an Illumina HiSeq 4000 75PE sequencing system.
GEO Accession
GSM3693263