GSM4680579: anaerobically grown cells rep 3; Acidithiobacillus ferrooxidans; RNA-Seq
The total RNA was extracted with a TRI reagent (Sigma-Aldrich) and was treated with a TURBO DNA-free kit (Ambion) to remove the contaminating DNA. The cDNA libraries were constructed using the ScriptSeq™ Complete Kits (Bacteria) (Illumina), including Ribo-ZeroTM technology for ribosomal RNA removing and ScriptSeq v2 RNA-Seq Library Preparation Kit. Sequencing was performed on the Illumina MiSeq platform with MiSeq Reagent Kit v2 (500-cycles), which generated 250-bp paired-end reads.
GEO Accession
GSM4680579