GSM3325809: snR4_Mutant_Glucose_1; Saccharomyces cerevisiae; RNA-Seq
From total RNA samples (10mkg total RNA), poly(A)-tailed RNAs were selected using Dynabeads™ mRNA Purification Kit (Thermo Fisher Scientific) according to manufacturer's protocol. RNA-seq cDNA libraries were prepared with NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina according to the instruction manual provided (https://international.neb.com/-/media/catalog/datacards-or-manuals/manuale7420.pdf) using 50-100ng Poly(A)-selected RNA samples as starting material. NEBNext Multiplex Oligos (NEB) adaptors and primers were used to multiplex cDNA Illumina libraries.
GEO Accession
GSM3325809