GSM4435003: KD mH2A1.2 rep2; Mus musculus; RNA-Seq
The transcriptomic of myotubes at day 4 was analysed. RNA was extracted (as described in the section "RNA isolation and cDNA synthesis") using the PureLinkTM RNA Mini Kit (Ambion, Life Technologies), including on-Column PureLink DNase treatment. RNA concentration was checked using Nanodrop (ThermoScientific). RNA was sent for library preparation to the CRG Genomics Unit (CRG Core Facilities). RNA quality control showed RNA integrity number (RIN) was between 9.9 and 10. RNA was amplified using mRNAseq which selects polyA. From 1ug of total RNA, the sequencing was performed on single read (SR), with an average length of reads of 50 bp, on an Illumina HiSeq2500 sequencer. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM4435003