GSM2582391: ChIPseq for HP1g (Cbx3) in Adnp knock-out mES cells, rep3; Mus musculus; ChIP-Seq
10^7 mES cells were crosslinked, chromatin was extracted and sonicated to an average size of 300bp. 1% of chromatin was set aside as Input control. The chromatin was incubated with Streptavidin-coupled Dynabeads, washed and de-crosslinked. Purified DNA was subsequently used for library preparation. For a detailed protocol, see Ostapcuk et al, 2017. Libraries were prepared using the NEB Next DNA library prep kit according to manufacturer's instructions. For barcodes, see sample characteristics.
GEO Accession
GSM2582391