Mixed oligochaetes sample 4
The total genomic DNA was extracted from a mixed samples of oligochaete fragments using the Qiagen DNeasy Tissue Kit (Qiagen, Hilden, Germany). The fragment of COI gene was PCR amplified. The extract was PCR amplified. The PCR products were purified individually using High Pure PCR Purification Kit (Roche Diagnostics) and appended with Illumina PE adapter sequences. This was performed using the TruSeq DNA Nano kit (Illumina) and following the kit instructions to include a unique index as a label for each library. The library was sequenced along with the other libraries of the BioProject on one MiSeq paired-end sequencing run of 2*251 cycles using a MiSeq Reagent Nano Kit v2 (illumina).