GSM3175254: A3; Borreliella burgdorferi; OTHER
DNA was isolated from a pool of three independent, late log-phase cultures (5 ml per culture) for each B. burgdorferi strain by standard phenol-chloroform extraction, concentrated by precipitation with isopropanol, and cleaned using Agencourt AMPure XP beads (Beckman Coulter). Libraries were generated using a 20kb shear protocol, loaded onto one SMRT cell per sample, and sequenced on a PacBio RSII instrument using P6-C4 sequencing chemistry and a 360-minute data collection protocol.
GEO Accession
GSM3175254