16S rRNA amlicon microbial consortium of bioreactor without lime treatment
The V4-V5 fragment of the 16S rRNA gene was amplified with 515f-806r primers following the illumina manufacture instruction and as described by Caporaso JG  et al. (Caporaso JG, Lauber CL, Walters WA, Berg-Lyons D, Huntley J, Fierer N, Owens SM, Betley J, Fraser L, Bauer M, Gormley N, Gilbert JA, Smith G, Knight R. 2012. Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms. ISME J.).