GSM3619660: MMA-3D-2; Homo sapiens; RNA-Seq
Total RNA was isolated using the PureLink RNA isolation kit (Life Technologies) and contaminant DNA was digested with DNAse I (Amplification grade, Sigma-Aldrich) "Active Motif" processed 1 µg of total RNA (RIN values between 8.7-10.0) to obtain polyA RNA. The TruSeq RNA Sample Preparation v2 Guide protocol was followed (Illumina, Part # 15026495 Rev. F) as per the manufacturer indications. Libraries were sequenced on Illumina NextSeq 500 as paired-end 42-nt reads, to a depth of 40.2 – 54.7 M read pairs.
GEO Accession
GSM3619660