metagenome sequencing of scabies mite: adult females
The metagenomes of washed scabies adult mites were prepared using Nextera DNA Sample Preparation Kit (Illumina, San Diego, CA, USA). gDNA was fragmented and end-tagged with an adaptor sequence. Tagged fragments were purified and amplified in a limited cycle PCR (5 cycles). Libraries were pooled, clustered using the cBot (Illumina) and sequenced paired end for 300 cycles by HiSeq2500 system (Illumina).