Carcinus maenas transcriptome - hepatopancreas
RNA was extracted using Qiagen’s miRNeasy mini kit as per manufacturer’s instructions, with on column DNase digestion. RNA quality and integrity was checked, and quantity measured using                     Agilent Bioanalyser. cDNA libraries for each tissue were constructed using equal amounts of RNA from four individuals that were pooled together. ERCC Spike-In control mixes were added to  control for technical variation during sample preparation and sequencing.  mRNA purification was performed via poly(A) enrichment using Tru-Seq low throughput protocol and reagents  (Illumina) and finally cDNA libraries   constructed using Epicentre’s ScriptSeq v2 RNA-seq library preparation kit.