GSM2759022: HeLa_U1_AMO_8hr_PolII_ChIP; Homo sapiens; ChIP-Seq
Cells were harvested and then crosslinked with 1% Formaldehyde. Nuclei were chemically isolated and then lysed, then sheared using a sonicator. The resulting sample was cleared using dynabeads, and polII-DNA complexes were isolated with the antibody. Libraries were prepared according to instructions accompanying the TruSeq ChIP sample preparation kit from Illumina (# IP-202-1012). Briefly, the overhanging DNA ends were blunted using exonuclease, and then the 3' end was adenylated to prevent improper ligation. The adapters were then ligated, and the library was amplified.
GEO Accession
GSM2759022