GSM4002964: H3K27ac_HiChIP_NIH3T3_2; Mus musculus; OTHER
H3K27ac HiChIP was performed according to (Mumbach et al., 2017) with only minor modifications. Approximately 15 million NIH3T3 cells were used as input, and MboI digestion was carried out for 2 hours. We used anti-H3K27ac (Abcam, ab4729) . Cells were sonicated for 10 cycles (30 seconds on, and 30 seconds off) using a Bioruptor Pico instrument (Diagenode). For biotin pull down 150ng of chromatin was used as input, and tagementation was performed using 4 uL of Tn5 transposase. For post-PCR size selection we performed a double-sided size selection with Ampure XP beads (Beckmann-Coulter). All libraries were sequenced on a NextSeq 500 platform.