GSM3163824: MPRA_rep2; Homo sapiens; OTHER
Cell nuclei were isolated as described previously (Buenrostro et al., 2013), lysed in RLT plus buffer (Qiagen) and stored at -80C. Genomic DNA and RNA were extracted using AllPrep DNA/RNA mini kit (Qiagen). 24ug RNA and reverse-transcribed using SuperscriptII. The cDNA and 8ug genomic DNA were used for PCR. PCR products were size-selected with an agarose gel, purified and pooled.
GEO Accession
GSM3163824