GSM4629078: ATAC-seq_Pulmonary artery endothelial cells - caMEK5_RepA; Homo sapiens; ATAC-seq
Cells were trypsinized, washed with ice-cold PBS, and lysed to isolate the nuclei. Isolated nuclei were incubated at 37°C for 30 min in the presence of 5 ul of TDE1 (Nextera Tn5 transposase, Illumina), followed by PCR amplification and gel purification as previously described (Buenrostro et al. Nat Methods 2013)
GEO Accession
GSM4629078