GSM4192134: H9.KO3.3.1_RNA-seq; Homo sapiens; RNA-Seq
Total RNA extraction by non ionic detergent-based cell lysis was performed for both H9 and HEK293 cells. Libraries for H9 WT and NSun6 KO cells (at least 4 replicates each) were performed as described before (Bornelov et al, 2019, Blanco et al. 2016). For the RNA seq samples, we used the NEB ultra-directional library prep kit (NEB). Ribosomal profiling samples were made into libraries using the Illumina Truseq small RNA kit (Illumina). HEK293 WT and NSun6 KO libraries (4 replicates each) were prepared using the Illumina TruSeq Ribo Profile kit; the Total RNA libraries were depleted of ribosomal RNAs using the Illumina Ribo-Zero rRNA removal kit.
GEO Accession
GSM4192134