DNA sequencing of Busseola fusca adult leg tissue
Six legs from a single adult individual were used for DNA extraction. We extracted genomic DNA using Qiagen DNeasy Blood & Tissue Kit (cat. no. 69504). We prepared two libraries for sequencing from the sample taken from a single individual using a Nextera XT DNA Library Prep Kit (Illumina). We performed next-generation sequencing using the Illumina MiSeq platform at the Biosciences eastern and central Africa (BecA) genomics platform at the International Livestock Research Institute (ILRI) in Nairobi, Kenya.