C3xAlt_POOL4
Pooled multiplex fragment libraries of 23 individuals from a segregating F1 population generated from the cross of globe artichoke "C3" genotype and cultivated cardoon "Altilis 41" genotype. DNA samples were fragmented to lengths of ~300 bp by sonication using a Covaris E220 as per the manufacturer’s instructions (Covaris, Inc.). Genomic paired-end libraries were then prepared using standard procedures and NEB reagents for end-repair, A-tailing, and adapter ligation. Fragment size was controlled using Ampure beads purification procedure. Libraries were PCR-enriched using the high-fidelity Phusion polymerase with 5 cycles of amplification. These libraries were prepared with the aim to perform shallow coverage sequencing for genotype imputation purposes.  Individuals are identified with the following barcodes in the header: Art_178:ACTGAT; Art_179:ATGAGC; Art_180:ATTCCT; Art_181:CAAAAG; Art_182:CAACTA; Art_183:CACCGG; Art_184:CACGAT; Art_185:CACTCA; Art_186:CAGGCG; Art_187:CATGGC; Art_188:CATTTT; Art_189:CCAACA; Art_190:CGGAAT; Art_191:CTAGCT; Art_192:CTATAC; Art_193:CTCAGA; Art_194:GCGCTA; Art_195:TAATCG; Art_196:TACAGC; Art_197:TATAAT; Art_198:TCATTC; Art_199:TCCCGA; Art_200:TCGAAG