GSM2584457: rat_Tail tip; Rattus norvegicus; Bisulfite-Seq
Genomic DNA was isolated from the tissues and cells with Universal Genomic DNA Extraction Kit (TaKaRa). Reduced representation bisulfite sequencing (RRBS) libraries were constructed as described previously with some modifications.Briefly, about 1 ug genomic DNA was digested with the MspI enzyme. Digested DNA was then purified, end-repaired, and adenylated, followed by ligation to Illumina TruSeq barcode adapters. Fragments of 150-300 bp in size were selected, bisulfite-treated, and sequenced using an Illumina Hiseq 2500.
GEO Accession
GSM2584457