GSM4191565: 12B; Homo sapiens; RNA-Seq
total RNA  was extracted with TRIzol® Reagent (Life technologies) RNA sequencing libraries were prepared from 2μg of total RNA using the KAPA RNA HyperPrep Kit with RiboErase (HMR) for Illumina®（Kapa Biosystems, Inc., Woburn, MA）. Instead of purifying poly-A RNA using poly-dT primer beads, we removed ribosomal RNA using the Ribo-Zero rRNA Removal Kit (Illumina, Inc.). Linear RNA was removed using RNase R (Epicentre, Inc). Next, the  RNA was fragmented, and then first-strand and directional second-strand syntheses were performed. Subsequently, a tailing/adapter ligation approach was performed with the purified cDNA. Finally, the purified, adapter ligated DNA was amplified. Each library was diluted to 10 nM and pooled equimolar prior to clustering.All of the steps were performed according to the manufacturer's protocols.
GEO Accession
GSM4191565