Illumina HiSeq 2500 sequencing; RNA-seq of Arabidopsis Col-0, snrk2.4, double snrk2.4/2.10 and quintuple snrk2.1/2.4/2.5/2.9/2.10 mutants from control (0mM) and salt stress (150mM NaCl for 1hour) treatment.
RNA-seq of Arabidopsis Col-0, snrk2.4, double snrk2.4/2.10 and quintuple snrk2.1/2.4/2.5/2.9/2.10 mutants from control (0mM) and salt stress (150mM NaCl for 1hour) treatment.
Arabidopsis thaliana seeds were surface sterilized with 20 ml thin bleach and 600 μL 37,5% HCl for 3 hours and then placed for 1.5 hour in laminar flow to evaporate chlorine gas. Seeds were stratified for 72 hours at 4°C and grown under long day conditions (21°C, 70% humidity, 16/8 light/dark cycle) in 100 ml liquid media containing 0.5xMurashi-Skoog basal salt, 0.5% sucrose, 1% M.E.S. monohydrate, pH 5.8 (KOH) with shaking (120 rpm). Ten days-old seedlings were treated with 150 mM NaCl in 0.1xMS media (salt stress) or 0.1xMS media (control) for time indicated separately per experiment. Seedlings were dried with paper towel and snap frozen in liquid nitrogen.  100 mg of tissue was ground and total RNA was extracted using Plant RNA extraction kit (Qiagen) according to manufacturer's instructions RNA quality determination, library preparation and sequencing with Illumina HiSeq 2500 was performed by Eurofins Genomics (Germany).
Experimental Factor: compound
sodium chloride
Experimental Factor: dose
150
Experimental Factor: genotype
snrk2.4/2.10 double mutant