GSM2769520: MBO22 (C) rep3; Streptococcus pneumoniae; RNA-Seq
RNA was extracted from bacteria pellets with lysozyme, followed by clean-up and DNase I treatment with NucleoSpinRNA II kit kit per manufacturer's recommendations. The ribosomal RNA was removed from the total bacterial RNA using ribosome reduction for Gram positive bacteria (ArrayStar, Rockville MD).  The purified RNA was then processed using the SureSelect Strand Specific RNA-Seq library prep kit from Agilent Technologies (San Diego, CA) following the manufacturer's protocol. The resulting libraries were quantitated with quantitative PCR (KapaBiosystems, Woburn MA) and the size distribution of the inserts were checked on the Agilent 2100 BioAnalyzer on a High Sensitivity DNA Chip. Library concentration was normalized to 2nM
GEO Accession
GSM2769520