GSM1531214: RNA110714RH_21; Mus musculus; RNA-Seq
Brain striatum region was hand-dissected and frozen. Total RNA was harvested by using manual homogenization using Trizol reagent (Invitrogen), mortar and pestle. It was then precipitated using sodium acetate after isolation. RNA libraries were prepared for sequencing using TruSeq PolyA Illumina protocol: PolyA(+) RNA was recovered from 1 ug of total RNA using oligo-dT attached to magnetic beads. PolyA(+) RNA was chemically fragmented. Double stranded DNA was made from the fragmented RNA using random hexamers. All ends were repaired.  A single A was added to each of the 3’ ends of each fragment. Illumina adaptors were ligated to the fragments.  Concentration was determined by real time PCR. Samples were mixed for multiplexing. Samples were diluted to target 180 million templates per lane on the flow cell
GEO Accession
GSM1531214