16 SMRT cells of Drosophila yakuba Ta18E2 PacBio RS II reads
High Molecular Weight DNA extraction with QIAgen Blood and Tissue kit (Genomic-tip 100/G), sheared with 24 gauge blunt-end needle (Chakraborty et al. 2015 BiorXiv), verified length distribution by FIGE, submitted 20 ug for 1 library preparations using BluePippin 15-80kb size selection, standard PacBio 20kb library preparation without Covaris G-tube step, followed by final BluePippin 15-80kb size selection of SMRTbell templates. Sequenced 16 SMRT cells, totalling ~11.8 Gb. The library prep resulted in 2 runs of the PacBio RS II sequencer: a Titration run (R148) of 2 SMRT cells (to determine optimal loading concentration), and a Main run (R149) of 14 SMRT cells at the optimal loading concentration determined by the titration run. R148-F02.1 was loaded at 300 pM, the rest were loaded at 400 pM.