GSM4191906: EK02009: HT29CaSR-GFP + DMSO; Homo sapiens; RNA-Seq
Cells were harvested and lysed in TRIZOL and RNA was column purified using RNAmini prep (QIAGEN). Sequencing libraries were prepared using the NEBNext Poly(A) mRNA Magnetic Isolation Module, the NEBNext UltraTM II Directional RNA Library Prep Kit for Illumina and the NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1) according to manufacturer's protocols (New England Biolabs). Single-end stranded mRNA-Seq. Pooled libraries were sequenced on a NextSeq500 instrument (Illumina) in 1x75bp single-end sequencing mode.
GEO Accession
GSM4191906