GSM3938115: Rojo_Trat10d_5; Vanilla planifolia; RNA-Seq
For the total RNA extraction from the roots of Vanilla planifolia Jacks, a protocol was standardized based on what was described by Valderrama-Chairez et al., 2002; which included the extraction of total RNA from 200 mg of root tissue with the help of Trizol Reagent, a treatment with Phenol: chloroform: Isoamyl Alcohol 25: 24: 1, and the subsequent cleaning of the RNA obtained, using silica columns included in the SV Total RNA Isolation System extraction kit from Promega. TruSeq stranded mRNA sample preparation kit.