RNA-Seq fom meiocytes of the barley cultivar Golden Promise
Around 30-50 anthers of 0.5  0.9 mm size were collected. The tips of the anthers were cut with insulin needles and were tapped gently to release the meiocytes bag. Using RNase-free 10 l tips, released meiocytes were transferred into a new 0.5 ml Eppendorf tube containing 10 l of citrate buffer and anther tissue was discarded. RNA was extracted using TRIzol PlusRNA Purification Kit (Thermofisher Scientific) following the manufacturers instructions). RNA-seq libraries were constructed using TruSeq mRNA Sample Preparation kit (Illumina) as recommended by the manufacturer. Libraries were quality checked using a Bioanalyzer 2100 (Agilent) and quantified by both qPCR (KAPA Library Quantification Kit, KAPA Biosystems) and using a Qubit fluorometer.