GSM3100615: ST2734-2; Streptococcus pneumoniae; RNA-Seq
Bacteria were harvested by centrifugation and were disrupted using a beadbeater (BIoSpec). RNA was prepared using a trizol-based protocol and treated with DNase using Rnase-free Dnase Set (Qiagen). Libraries were constructed using ScriptSeq complete kit (Bacteria)(Epicentre) following the manufacturer's mannual. rRNA was removed using Ribo-Zero magnetic kit (Epicentre). Library was generated using ScriptSeq Complet Kit (Bacteria) (Epicentre) following the manufacturer's mannuals. Samples 1 to 6 were amplified with index PCR primers 1 to 6, respectively, from Script Seq Index PCR primers (Epicentre).
GEO Accession
GSM3100615