GSM2839431: small RNA ISO rep3; Rattus norvegicus; ncRNA-Seq
Total RNAs are extracted by Trizol (Invitrogen). 1-2 μg total RNAs were separated by denatured 15% PAGE with 7M urea. The gel was stained with SYBR Green I solution (Invitrogen). Small RNAs sized at 15-40 nt were excised from the gel were uesd to construct small RNA-sequencing library and sequenced on an Illumina Hi-Seq 2000 apparatus. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM2839431