i4C on WT KB cells, viewpoint CXCL2 enhancer
Preparation of i4C templates (~5 million cells), using ApoI as the primary restriction enzyme and the promoter/enhancer regions of IL8 and CXCL2 as viewpoints (Brant et al. (2016)). We performed PCR-based generation of i4C libraries (HiSeq2500 platform (Illumina), Stadhouders et al., 2013)) and sequencing to 8 million reads.