RNA_pulse_4sU C 2h Total
MCF-7 cells were seeded 48 hrs prior to the experiment at a cell density of 0.3 x 105 cells/ cm2. Cells were labeled with 4-thiouridine (4-SU) (Sigma-Aldrich) at a final concentration of 200 M for 0, 2, 4 or 8 hrs.