Corky tomato rep1
PCR reactions were conducted using total genomic DNA and Kappa polymerase (Kappa HiFi Hotstart Ready Mix, cat. No. KK2602, Kappa Biosystems). PCR reactions were cleaned up using magnetic beads (BeckmanCoulter Agencourt Ampure XP, cat. No. A63881) and resuspended in 10 mM Tris pH 8.5 or Resuspension buffer (RSB, Illumina Inc.). Libraries were constructed using Nextera XT adapters (Illumina Inc.) using the same polymerase and cleaned again using magnetic beads according to specifications. Libraries were quantified by fluorescence using a Qubit DNA HS kit, library quality was analyzed using a Tapestation 2200 or Bioanalyzer 2100 and pooled to an equimolar ratio to be sequenced in a 2X300 bp MiSeq Run.