21r_5WEEK_roots_400PPM_CO2_16S
Total DNA was extracted from 0.4 g roots or 0.3 g soil, using the Exgene Soil DNA mini isolation kit (GeneAll) according to the manufacturer's instructions. Total bacterial community structure was characterized using high-throughput sequencing of the V4 variable region of microbial small subunit ribosomal RNA (16S rRNA) amplicon.