GSM3391785: Control_Input; Mus musculus; ChIP-Seq
In vitro cultured GC B cells from STAT3 control and cKO mice were used for ChIP studies as described previously (Tripathi et al. 2017. Cell Reports). In brief, cells were subjected to sonication using a sonicator (AFA Focused ultrasonicator S220) to obtain chromatin fragements of 100-500 bp. Fragemented chromatin was incubated with STAT3 Ab (124H6, Cell Signaling Technology) or isotype control Ab and incubated for crosslinking with the beads (Dynabeads Protein G, Invitrogen). After crosslinking, crosslinks were reversed (65C for 12-16 h), and precipitated DNA was treated with Proteinase K and then purified (QIAquick PCR purification kit, Qiagen). DNA was DNA-end repaired, with a 3'-dA overhang and ligation of methylated sequencing adaptor.  Sequences were PCR amplified and size selected in the range of 100-500bp (including adapter sequence).  Selected sequences were then prepared using the BGISEQ-500 ChIP-Seq sample preparation workflow.
GEO Accession
GSM3391785