Staphylococcus pseudintermedius strain 157588
The paired-end sequencing library was prepared using NEBNext Ultra DNA Library Prep Kit for Illumina. The library preparation process was initiated with 200ng DNA. The DNA sample was mechanically sheared into smaller fragments by Covaris followed by continuous step of end-repair where an A is added to the 3 ends making the DNA fragments ready for adapter ligation. Following this step, platform-specific adapters are ligated to both ends of the DNA fragments. These adapters contain sequences essential for binding dual-barcoded library to a flow cell for sequencing, allowing for PCR amplification of adapter-ligated fragments, and binding standard Illumina sequencing primers. To ensure maximum yields from limited amounts of starting material, a high-fidelity amplification step was performed using HiFi PCR Master Mix.