RNA-seq for adult L.taibai
RNA extraction was performed using an RNeasy Mini Kit (Qiagen, Hilden, Germany) in accordance with the manufacturers instructions. Two cDNA libraries, having inserted sequence sizes of 150 kb, were constructed for sequencing on a Hiseq 4000 platform (Illumina, San Diego, CA, USA). Procedures for the construction of cDNA library were as follows. Briefly, approximately 3 g total RNA was used for each sample. mRNA purified from total RNA was enriched using poly-T oligo attached magnetic beads and then randomly fragmented. These fragments were used for cDNA synthesis using random hexamers. Following fusion of doublestranded cDNA, the products were purified using AMPure XP beads (Beckman Coulter, California, USA). cDNA libraries with reads of 150200 bp were filtered out using an AMPure XP system.