GSM3172971: SOX2_PAR-CLIP_rep2; Homo sapiens; RIP-Seq
Samples were treated with proteinase K (Merck), and RNA was isolated using phenol/chloroform extraction. cDNAs were synthesized with SuperScript III (#18080093) and amplified with a 18 cycles PCR. The PCR products were selected based on size using from PAGE gels according to instructions of TruSeq® Small RNA Sample Prep Kit (Illumina, Cat#15019892). The purified library products were evaluated using the Agilent 2200 TapeStation and diluted to 10 pM for cluster generation and high-throughput sequencing (50 bp single end) on HiSeq 2500 (Illumina).
GEO Accession
GSM3172971