Illumina HiSeq 2000 sequencing; Whole transcriptome sequencing of splenic influenza-specific CD8+ T cells in wt and Notch1/2ko mice
Whole transcriptome sequencing of splenic influenza-specific CD8+ T cells in wt and Notch1/2ko mice
Total RNA was extracted with TRIzol reagent (Invitrogen) according to the manufacturers protocol. Total RNA was further purified by nucleospin RNAII columns (Macherey-Nagel). RNA was amplified using the Superscript RNA amplification system (Invitrogen) and labeled with the ULS system (Kraetech), using either Cy3 or Cy5 dyes (Amersham).
Experimental Factor: cell type
KLRG1+ CD127- H-2 Db-NP366-374+ CD8+ T cell
LIBRARY_STRAND
not applicable