GSM3938986: U2OS.+HHV6A.+TSA.A; Homo sapiens; miRNA-Seq
Cells were washed once with PBS and total cellular RNA was extracted using Qiagen RNeasy mini kit. RNA-seq libraries were prepared with the TruSeq Stranded mRNA LT sample preparation kit (Illumina) using both, Sciclone and Zephyr liquid handling robotics (PerkinElmer). Library concentrations were quantified with the Qubit 2.0 Fluorometric Quantitation system (Life Technologies) and the size distribution was assessed using the Experion Automated Electrophoresis System (Bio-Rad). For sequencing, samples were diluted and pooled into NGS libraries in equimolar amounts and were sequenced using NextSeq 500.