Whole-exome sequencing of TET1-deficient tumors
Genomic DNA underwent enrichment for the mouse exome through the use of a SeqCap EZ design mouse exome capture kit (Roche NimbleGen). The coding sequence selected for the mouse exome probe pool design includes 203,225 exonic regions, including microRNAs, and collectively comprises over 54.3 Mb of target sequence. Sequencing libraries were generated from captured exomes by standard Illumina protocols and were sequenced on the Illumina HiSeq 2500 using 100 bp paired-end reads.