GSM2838604: Set1-3; Saccharomyces cerevisiae; OTHER
Cells were harvested, frozen in liquid nitrogen, and lysed cryogenically using a Retsch Oscillating Mill MM400. 3XFLAG tagged Dbp2 or Set1 and bound RNAs were immunoprecipitated using M2 anti-FLAG antibody. After immunoprecipitation, samples were ligated with a biotinylated 3' adaptor, size-selected by SDS-PAGE, digested with proteinase K, and RNAs  were extracted using acid phenol/chloroform. cDNAs were generated using TGIRT-III enzyme and circularized using CircLigase II (epicentre). After purified using MyOne Streptavidin C1 Dynabeads, cDNAs were amplified using SYBR master mix (Applied Biosystems) and gel-purified.
GEO Accession
GSM2838604