Ion Torrent S5 sequencing
Targeted resequencing of exons from a panel of 751 genes considered to be important for neurological disease.
Genomic DNA was extracted from peripheral whole blood using Nucleo Spin Blood XL kits (Macherey-Nagel Duren, Germany), and subjected to Ion Ampliseq (Thermo-Fisher, Waltham, MA) targeted amplification of exons per the manufacturers� protocols for sequencing on the Ion S5 instrument.