RNA_seq of Euphorbia lathyris latex
Total RNAs were extracted from 300 microL of fresh latex collected from 3-month-old greenhouse grown Euphorbia lathyris plants. Plant stems were sectioned in apical, middle, and bottom parts in order to favour the outflow of the latex. After an initial post-sectioning bleeding of several drops of latex, the remaining fluid was blocked inside the tissues due to capillary forces. Such a sampling was very efficient to generate pure latex. In brief, purity of total RNAs was achieved by phenol/chloroform phase extraction followed by a LiCl precipitation step according to standard procedures. The final cleanup of samples was performed with the RNeasy Plant Mini Kit (Qiagen). Quality control of the latex RNAs was done with the Agilent 2100 Bioanalyzer. RNA samples for transcriptome analysis consisted of 2.5 microg of RNAs from apical parts latex and 2.5 microg of middle parts latex. Those 5-microg samples were used to prepare libraries for paired-end Illumina Genome Analyzer II (Solexa) sequencing according to the manufacturer instructions.