Small RNA-Seq of Leptinotarsa decemlineata: adult whole control insect
Small RNA fractions (n = 4) quality was assessed on Agilent TapeStation using standard RNA ScreenTape assay. miRNA library was prepared from 250 ng of RNA using RNA-Seq v2 kit (Life Technologies). Amplifed samples were purified and size selected to enrich for small RNA templates using Nucleic Acid Binding Beads (Life Technologies).Quality of the library was analyzed using Taqman library quantification assay (Life Technologies) (Ion Library TaqMan Quantitation Kit, Simi?). Barcoded libraries were equally pooled and amplified onto Ion Sphere Particles (ISPs) supplied by Ion PI Hi-Q OT2 kit (Life Technologies). ISPs enriched with template library were loaded onto Ion PI chip v3 and sequenced