GSM4679353: Vg1 RNP; Xenopus laevis; RNA-Seq
Briefly, oocytes were manually breaked down to get the extract and the Biotin-Vg1RNP was pulled down by incubating with streptavidin-conjugated beads. RNA was extracted by phenol and precipitated by ethanol. Library was prepared using the NEBNext Ultra II Directional RNA library prep kit with RiboZero depletion module H/M/R.
GEO Accession
GSM4679353