GSM4005295: Mir_rep1; Mus musculus; RNA-Seq
RNA was extracted using Arcturus PicoPureTM RNA isolation kit (Thermo Fischer Scientific, Cat # KIT0202) according to the manufacturer´s protocol. RNA integrity was analysed using Bioanalyzer (Agilent 2100) and samples with RIN values above 9.5 were selected for RNA sequencing. For each biological replica, cells from two embryos were pooled together, and three independent replicas per condition were sequenced. Libraries were prepared with the SMART-seq v4 Library Prep Kit and sequenced on Illumina HISeq 2500 sequencer using 50bp single reads.
GEO Accession
GSM4005295