GSM3391945: E195_KO7-1; Mus musculus; RNA-Seq
Total RNA was extracted from tissues with TRIzol (Invitrogen). Following extraction, 1 μg of purified RNA was used for reverse transcription polymerase reaction each time (Invitrogen). For RNA-seq library construction, two rounds of PolyA+ tailed RNA purification were performed for each sample. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM3391945