GSM1904118: WGBSeq EpiLC 1; Mus musculus; Bisulfite-Seq
EpiLCs were induced for 48h from 2i/LIF ESCs as previously described (Hayashi et al., 2011). 160 ng of sonicated DNA was used for library preparation using Ovation Ultralow Methyl-Seq Library System (Nugen). This entailed a 3.5 h bisulfite conversion of adapter-ligated DNA using EZ DNA Methylation-Direct (Zymo Research). The DNA was amplified using 7 cycles. Once libraries were prepared they were sequenced using HiSeq2000 with paired-end 100nt read length.
GEO Accession
GSM1904118