GSM4433070: Wild type oocyte rep3; Mus musculus; Bisulfite-Seq
The oocytes were lysed in buffer containing 0.5 µl proteinase K for 1 h at 37°C. DNA was converted by the EZ DNA Methylation-Gold kit (Zymo Reserch) according to the instructions. Then the converted DNA was subjected to library construction. The purified libraries were assessed for quality using High-Sensitivity DNA chips on the Agilent Bioanalyzer 2100 and StepOnePlus RealTime PCR System. The libraries were sequenced on one lane of an Illumina HiSeq 2500 for 140-bp paired-end sequencing.
GEO Accession
GSM4433070