RNA-seq of Aplysia california: 7 months BSC
total RNA extracted with Qiagen RNEasy Micro-kit from homogenized sensory neuron clusters. Libraries generated with Illumina TruSeq mRNA Stranded HT kit. Total RNA was poly-A selected with oligo-dT beads. First strand synthesis was random primed. Libraries were multiplexed and sequenced across 8 lanes on an Illumina HiSeq 2500 as paired end 100 base pair reads.