DNA seq of Stenotrophomonas maltophilia D457 fosfomycin resistant mutant FOS1
Chromosomal DNA from a Stenotrophomonas maltophilia fosfomycin resistant mutant (FOS1) was obtained from an overnight culture. DNA was mechanically fragmented and libraries prepared using the TruSeq DNA Sample 7 Preparation v.2 Kit. Briefly, the DNA was made blunt ended, phosphorylated, and an adenine added in the 5-end of the fragments so that Illumina adapters could be ligated. Libraries including adapters were gel-purified and PCR-amplified using common primers so a sufficiently large library could be subjected to sequencing. Libraries were subjected to single-end sequencing with a read-length of 1x150 and a coverage between 26 and 41X in a MiSeq Illumina instrument.