DNA-Seq of Sphenodon punctatus: adult male blood: 2x250 PE 480 insert
Total genomic DNA was extracted using proteinase K digestion and Phenol-Chloroform extraction from blood. Sequencing was undertaken using the Illumina MiSeq sequencing platform (Illumina, San Diego, CA, USA). The sequencing library consisted of a paired end (PE) library with estimated insert sizes of 480 bp. The paired end library was prepared using the Illumina TruSeq DNA library kit as per manufacturers instructions. The library was run on one lane of Illumina MiSeq using 2 x 250 bp paired end sequencing at New Zealand Genomics Ltd., Dunedin.