GSM3323787: SLE280.aN [RRBS-seq]; Homo sapiens; Bisulfite-Seq
DNA was extracted from FACS isolated cell populations using the AllPrep DNA/RNA Mini Kit (Qiagen). Two aliquots of DNA were separately digested with either TaqI or MspI restriction enzymes over night and subsequently pooled for all downstream processing.  Digested DNA was end-repaired, A-tailed, and adapters containing methylated cytosines ligated on using the KAPA HyperPrep kit according to the manufacturers instructions.  Adapter-ligated DNA was bisulfite converted over using the EpiTech Bisulfite kit (Qiagen, Inc).  Bisulfite treated libraries were PCR amplified using indexed primers and the HiFi HotStart Uracil+ polymerase (KAPA Biosystems).
GEO Accession
GSM3323787