Illumina HiSeq 2000 sequencing; Transcriptional Profiling of human pluripotent stem cells and and derived tissues
Transcriptional Profiling of human pluripotent stem cells and and derived tissues
H9 embryonic stem cells were differentiated into definitive endoderm and then treated with FGF4/Chiron (a Wnt agonist) in order to generate intestinal organoids as previously described by  Spence, et al. Nature 470: 105-109 (2010) and McCracken, et al. Nature Protocols 6:1920-1928 (2011). RNA was extracted from samples and then prepared for RNAseq using the Illumina TruSeq mRNA method
Experimental Factor: cell type
human intestinal organoids derived from H9 stem cells
LIBRARY_STRAND
not applicable