16S rRNA gene sequencing of  bulk soil in field: waste rock at Westwood site with vegetation
Libraries for sequencing were  prepared according to Illuminas 16S Metagenomic Sequencing Library  Preparation guide, with the exception of using Qiagen HotStar Taq DNA  polymerase for the first PCR (amplicon PCR). The template specific primers  were (without the overhang adapter sequence): 515F-Y  (5-GTGYCAGCMGCCGCGGTAA-3) and 926R (5- CCGYCAATTYMTTTRAGTTT-3). The first  PCR (amplicon PCR) was carried out for 35 cycles with annealing temperature  of 50C. Diluted pooled samples were loaded on an Illumina MiSeq and  sequenced using a 500-cycles MiSeq Reagent Kit v2.