GSM4629198: Sox10+_48-50hpf; Danio rerio; RNA-Seq
Posterior tissue (Vagal and trunk domains) were harvested; Single cell suspension was prepared via digestion in 37°C 1X Accumax buffer (Sigma-Aldrich, A7089) for 30-45 minutes. Suspension was washed in Hank's Buffer (1x HBSS; 2.5 mg/mL BSA; 10μM pH8 HEPES), concentrated via centrifugation (200 rcf for 10 minutes at 4°C) in a small volume of Hank's buffer, and sorted for GFP+ cells via FACS. GFP+ cells were used as input for the 10X Genomics Chromium platform using the 10X Single Cell 3' V2 chemistry kit for 10,000 cells. RNA libraries were prepared for sequencing using standard Illumina protocols following the 10x Genomics pipeline
GEO Accession
GSM4629198