H. sapiens TruSeq rep1
Human embryonic kidney cells (HEK293T) were cultured and used for total RNA isolation. Total RNA library preparation was carried out according to the Illumina TruSeq small RNA library preparation protocol. A pre-adenylated 3'-adapter was incubated with total RNA in the presence of T4 RNA ligase 2, while the 5'-ligation was carried out with T4 RNA ligase 1 and a 5'-RNA adapter. Reverse transcription and cDNA amplification was carried out according to the manufacturers protocol (Illumina). High-throughput analysis of the libraries was done as single end run (150 nt) on a Illumina MiSeq System with the custom primer (5'-CACTGTCGGTACCGAGCTTGCATGGAGTCCTA-3').