GSM3325655: tum7-mut; Mus musculus; RNA-Seq
Tissue was taken from -80 C and placed onto dry ice, prior to lysis by submersion in RLT buffer (Qiagen), directly followed by homogenisation in a microcentrifuge tube using a pestle at room temperature. Total RNA was isolated using the RNeasy Mini Kit (Qiagen). mRNA libraries were prepared with the KAPA mRNA Hyper Prep kit (Roche Applied Science) and sequenced as 9-plex pools on the HiSeq 4000 (Roche Applied Science) and sequenced as 9-plex pools on the HiSeq 4000 (Illumina).
GEO Accession
GSM3325655