Whole genome sequencing of C. beijerinckii DSM 6423 AA mutant
Genomic DNA samples was fragmented using a COVARIS S2 sonicator, to a mean size of 700 bp. DNA Libraries were constructed from 1 g of this fragmented DNA, according to the Illumina DNA sample prep protocol (End-repair, A-tailing, ligation, PCR enrichment), on a Beckman SPRI-TE automate and using reagents from the SPRIworks Fragment library system I kit (Beckman Coulter). The final product were gel purified to achieve a library mean size of 700 bp. Library quality was assessed on an agilent Bioanalyzer instrument (Agilent) and sequenced on a Illumina MiSeq instrument, using a Paired-end 2250 bp protocol, with a MiSeq Reagent Kit v2 500 cycle (MS-102-2003, Illumina), according to the constructor recommendations.