GSM3937669: Yersinia_ctrl; Yersinia pestis; RNA-Seq
Total RNA was treated with RQ1 DNase (Promega) to remove DNA. The quality and quantity of the purified RNA were determined by measuring the absorbance at 260nm/280nm (A260/A280) using SmartSpec Plus (BioRad). RNA integrity was further verified by 1.5% agarose gel electrophoresis. A total of 2.5μg of total RNA was used for cDNA library preparation with the adaptors accordance to illumina protocol. Briefly, RNAs were ligated to 3' and 5' adaptor sequentially and reverse transcribed to cDNA and then PCR amplified (RNA 3' Adapter: /5rApp/ATCTCGTATGCCGTCTTCTGCTTG/3'NH2/, RNA 5' Adapter 5'GUUCAGAGUUCUACAGUCCGACGAUCNNN3'). Whole library was applied to 10% native PAGE gel electrophoresis and bands corresponding to 80-380 bp RNA insertion were cut and eluted.
GEO Accession
GSM3937669