RNAseq adult mouse brain
Brain cells were sorted by FACS and cDNA synthesised following the Smart-seq2 method. Libraries were prepared from 0.25ng cDNA and 12 PCR cyclyes using the Nextera XT kit (Illumina). Adapter-trimmed paired fastq reads were aligned to the mouse genome (GRCm38) using Hisat and outputted to a bam file using samtools