Complete genome sequences of Dickeya fangzhongdai infecting Phalaenopsis orchid (Phalaenopsis spp.) in China
For long sequencing with PacBio RS, SMRTbell DNA libraries of fragment sizes of >10 Kb, was prepared with fragmented genomic DNAs after the treatments of G-tubes (Covaris) and end-reparation. SMRT sequencing was performed on the Pacific Biosciences RSII sequencer (PacBio) according to standard protocols using the P4-C2 chemistry