Illumina HiSeq 1000 sequencing; Lotus japonicus response to Nod factor: wild type and nfre mutants
Lotus japonicus response to Nod factor: wild type and nfre mutants
3 days seedlings were moved to agar plates supplemented with 1/4 B&D media and susceptible zone of 14 days-old plants was harvested after specific treatment with water(Mock treated) or M.loti Nod factor 10-8M.  The total RNA was isolated from the susceptible zone (15 mm root pieces) using Nucleo spin RNA plant (Macherey-Nagel).  Total RNA (> 0.8 µg) from two biological replicas per sample was used by GATC Biotech (Germany) to prepare random primed cDNA library.
Experimental Factor: genotype
wild type genotype
Experimental Factor: stimulus
water (mock treated)