GSM3756581: dpn OE-rep1; Drosophila melanogaster; RNA-Seq
Drosophila midgut were removed and digested using Elastase (Sigma, cat. no. E0258). About 10000 ISCs labeled with green fluorescent protein (GFP) were sorted using FACSAria II cell sorter (Becton Dickinson) and RNA was harvested using the  Arcturus PicoPure RNA isolation kit (Applied Biosystems). The RNA was then amplified using Arcturus RiboAmp HS PLUS RNA ampliﬁcation kit (Applied Biosystems). RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM3756581