GSM3938738: 293_pCW57_PNUTSW401A_Dox_Spt5_Rep2; Homo sapiens; ChIP-Seq
Cross-linked chromatin was sonicated with a Bioruptor or Covaris Ultrasonicator 1-2 mg of cross-linked extract was immunoprecipitated, and prepared for illumina library construction by repair with Klenow, T4 DNA polymerase and T4 polynucleotide kinase, A-tailing with Klenow exo-, ligation to branched adaptors, SPRI bead purification of ~200bp fragments, and amplification (15-18 cycles) with Illumina Tru-seq primers using Phusion DNA polymerase.
GEO Accession
GSM3938738