GSM3175497: ChIP-seq control library mouse; Mus musculus; ChIP-Seq
midguts were dissected and treated with 3mM EDTA for 20minuites. Floating Epithelial tissue was collected and treated with 0.05% Trypsin, neutralized with FBS, and Epcam+ cells were enriched using magnetic antibody and fixed for subsequent chromatin pull down. Whole cell lysates were sonicated and incubated without any antibody overnight for determining background chromatin pulldown. The ThruPLEX DNA-seq Kit (Rubicon Genomics) was used to prepare libraries
GEO Accession
GSM3175497