RNA-Seq fom anthers of the barley cultivar Golden Promise
At least 120 anthers of 0.3 - 0.4mm size were collected and RNA was extracted using TRIzol PlusRNA Purification Kit (Thermofisher Scientific) following the manufacturers instructions). RNA-seq libraries were constructed using TruSeq mRNA Sample Preparation kit (Illumina) as recommended by the manufacturer. Libraries were quality checked using a Bioanalyzer 2100 (Agilent) and quantified by both qPCR (KAPA Library Quantification Kit, KAPA Biosystems) and using a Qubit fluorometer.