GSM3098182: CD8_w0__R103_K5705: CD8 before treatment 5; Homo sapiens; RNA-Seq
Total RNA from were extracted using RNeasy Micro kit (Qiagen). 0.2 ng of total RNA from each dose and biological replicate were reverse transcribed to cDNA with SuperScript® VILO™ cDNA Synthesis Kit. Then cDNA is amplified using Ion AmpliSeq™ technology. After primer digestion, adapters and molecular barcodes were ligated to the amplicons followed by magnetic bead purification. Libraries were then diluted to 75 pM and pooled equally, with eighteen individual samples per pool.
GEO Accession
GSM3098182