GSM4434565: control liver ECs; Mus musculus; RNA-Seq
Liver cell suspensions were obtained by collagenase perfusion and were spun down  to remove hepatocytes.  The supernatants were pelleted  and resuspended.  Isolated non-parenchymal cells (NPCs) were stained with SYTOX® Red to label dead cells and sent to FACS sorting.  Only live and GFP-positive cells were sorted with BD FACSAriaTM IIu . Samples were prepared according to the instruction of 10x Genomics Single Cell 3, Reagent Kits v3. Single cell suspensions in RT Master Mix  were  loaded onto the 10x Genomics Single Cell A Chip to convert poly-adenylated mRNA into barcoded cDNA.  Barcoded cDNA was amplified by PCR to generate a sufficient mass for library construction.
GEO Accession
GSM4434565