GSM2340061: improved_group_time_1_subject_4; Homo sapiens; RNA-Seq
Blood samples were collected into PAXgene blood RNA tubes and processed with PAXgene blood miRNA kit (Qiagen, Valencia, CA) for miRNA extraction. Quality and quantity of extracted RNA was assessed using the NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Wilmington, DE) and the Agilent Bioanalyzer 2100 Small RNA assay (Agilent Technologies, Inc., Santa Clara, CA). All samples were enriched for miRNA using standard recommendations for preparation of small RNA libraries protocol. Barcoded libraries were prepared with an input of 1-5ng of enriched small RNA using the Ion Total RNA-Seq Kit v2 (Life Technologies, Carlsbad, CA). Template-positive Ion PGM™ Ion Sphere™ Particles were prepared for sequencing using Ion PGM™ Template OT2 200 Template Kit v2 with pooled libraries optimized to 14pM.
GEO Accession
GSM2340061