Padlock Capture on coding regions of RNA transcripts of X-linked genes in 6-day differentiated F-M3-KO cells #1
The cells were differentiated in vitro for six days. Total RNA was isolated and cDNA was generated by reverse transcription using oligo (dT) primers. The cDNA sample was subjected to padlock SNP capture using a padlock probe library targeting SNPs selected from the coding regions of X-linked genes along the mouse X chromosome.