GSM3755585: CD3_100_rep1_SMART_Nxt; Homo sapiens; RNA-Seq
Total RNA from the cells was extracted using Qiagen's RNeasy Micro Kit The SMART-Seq v4 Ultra-low Input RNA Kit for Sequencing (Takara #634888) was used to generate high-quality full-length cDNA. For SMART_CC, The cDNA was sheared using the Covaris M220 focused-ultra sonicator (SonoLab version 7.1) with run settings for desired target size between 200-500bp. Library preparation was then performed following manufacturer's instructions for Low Input Library Prep Kit v2 (Clontech # 634899). For SMART_Nxt, The cDNA was normalized to the modified recommended input amount. The samples were then tagmented according to Nextera XT DNA Library Prep Protocol (Illumina # FC-131-1024). For Ampliseq, 3.5ul of RNA was used for input into the Ion Ampliseq Transcriptome Human Gene Expression Kit (Thermo Fisher # A26325), and libraries were prepared following manufacturer's instructions
GEO Accession
GSM3755585