GSM4434551: S2; Rattus norvegicus; miRNA-Seq
Rat plasma was centrifuged at 10,000 g for 70 min to isolate exosomes. The total RNA sample was extracted and subjected to agarose electrophoresis and Nanodrop quality inspection and quantification, and then a library was constructed. The quality of the library was determined using an Agilent 2100 Bioanalyzer. The sequencing libraries of different samples were mixed, and single-stranded DNA was generated by denaturing with 0.1M NaOH. Vendor instructions Perform 51-cycle sequencing on the Illumina NextSeq 500 sequencer. miRNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM4434551