GSM2340350: ctrlRNAi_M-GEN_S93; Onthophagus taurus; RNA-Seq
After dissection, all tissue samples were immediately placed in ice-cold Trizol and stored at -80ºC. After thawing to 4ºC, tissues in Trizol were homogenized with disposable polypropylene RNase-free pellet pestles, and total RNA was extracted using a standard phenol-chloroform protocol followed by RNeasy Mini spin column purification including on-column DNAse I digestion. Total RNA was quality checked using RNA ScreenTape TapeStation System and quantified with a Quant-iT RiboGreen Assay Kit. A total of 96 (2 treatments x 2 sexes x 4 tissues x 6 biological replicates) RNA Stranded RNA sequencing libraries were constructed using the TruSeq Stranded mRNA Sample Preparation Kit (Illumina) according to manufacturer's instructions. Libraries were quantified using a Quant-iT DNA Assay Kit (Thermo Fisher), pooled in equal molar amounts, and sequenced as single-end reads using a 75 cycles, high output kit on the NextSeq500 platform (Illumina).
GEO Accession
GSM2340350