Fungal microbiome profile of deadwood and underlying soil
The microbial community of decaying deadwood and its underlying soil was analysed in a microcosm experiment. Microcosms were set up in the Italian alps on a north- and south-exposed slope at 1900 m above sea level. In a destructive sampling design, decaying deadwood blocks (P. abies) and their underlying soil were sampled at time points 0, 12, 25, 52 and 104 weeks of decay. Total DNA was extracted and the fungal ITS2 region and the bacterial/archaeal V4 region of the 16S DNA were sequenced for microbiome profiling.