GSM4005332: 04wCHAC0; Equus caballus; RNA-Seq
Samples were collected immediately following euthanasia and were flash frozen in liquid nitrogen or placed in RNAlater (Qiagen, Valencia, CA) at 4°C for 24-48 hours prior to storage at -80°C. Frozen samples were pulverized and placed in TRIzol reagent (Invitrogen, Carlsbad, CA) prior to mechanical homogenization. RNA extraction was performed on spin columns using the RNeasy Micro Kit (Qiagen, Valencia, CA) per manufacturer instructions. Standard library preparation and sequencing (100 base-pair, paired-end) on an Illumina Hi-Seq sequencer (90 samples on a Hi-Seq 2500, 22 samples on a Hi-Seq 4000)
GEO Accession
GSM4005332