16S sequencing data of CKD rat model: Sample c9-7
To enable amplification of the V4 region of the 16S rRNA gene and add barcode sequences, unique fusion primers were designed based on the universal primer set, 515F (5- GTGYCAGCMGCCGCGGTAA-3) and 806R (5-GGACTACNVGGGTWTCTAAT-3), along with barcode sequences. Run amplicons from each sample on an agarose gel. Expected band size for 515f-806r is ~300-350 bp. Quantify amplicons with Qubit dsDNA HS Assay Kit (ThermoFisher/Invitrogen cat. no. Q32854; follow manufacturers instructions).