GSM4567199: Induced D6  gonad; Mus musculus; RNA-Seq
cells were dissociated enzymatically by papain for 30 mins and then harvested by centrifugation. Single-cell RNA sequencing libraries were prepared from the freshly isolated female mouse gonad tissues and ES-derived gonad cells by using Chromium Single Cell 3ʹ v2 Reagent Kits (10X Genomics) as previously described (Hashimoto et. al. PNAS, 2019). Briefly, the dissociated cells were mixed with the Single cell Master Mix and generated gel bead-in-emulsions (GEMs). The RNAs in each GEM were revers-transcribed and the resulting cDNAs were amplified by PCR. Illumina sequencer adapted sequencing libraries were constricted from the amplified cDNAs. Single-cell RNA sequencing libraries were sequenced with paired-end reads on the Illumina HiSeq X Ten platform.
GEO Accession
GSM4567199