GSM2759427: WT_a; Mus musculus; RNA-Seq
Retinas were dissected and immediately frozen using dry ice for storage at -80C. Retinas were processed for RNA using the PerfectPure RNA tissue kit (5Prime). Quality was assessed using the Agilent Bioanalyzer, and samples with minimum 8.0 RIN score submitted for library preparation. mRNA was extracted using the DynaI mRNA Direct Kit (Life Technologies). mRNA was fragmented and reverse transcribed to double stranded cDNA using random primers before the additon of Illumina adaptors. rRNA was depleted using Ribo-Zero (Epicentre).
GEO Accession
GSM2759427