GSM2584649: HGPS5.calc.short.3; Homo sapiens; RNA-Seq
NF and HGPS fibroblasts supplemented with 10-7 M 1,25D (1α,25-dihydroxyvitamin D3; Sigma-Aldrich) or FBS for 4 to 90 days were used to extract total RNA using Aurum Total RNA Mini Kit (Bio-Rad) with DNaseI treatment following the manufacturer’s protocol. IonXpressRNA barcoded sequencing libraries were constructed using the Eukaryotic Ribominus and Ion Total RNA-seq v2 kits (Life Technologies) according to the manufacturer's directions.
GEO Accession
GSM2584649