DNA sequencing of Nephromyces-enriched renal sac of Molgula occidentalis
Total DNA was isolated from the inside contents of a dissected renal sac of a single individual of M. occidentalis using CTAB/chloroform extraction. A sequencing library was made using the PrepX DNA Library Kit (Takara Bio, USA). The DNA library was sequenced (2x250 bp) in an Illumina MiSeq instrument (University of Rhode Island).