GSM1903807: Treated root1; Avicennia officinalis; RNA-Seq
Total RNA was extracted from roots of control and treated (500mM NaCl for 24h) greenhouse-grown mangrove plants using Qiagen RNeasy kit (QIAGEN) and DNase treated (RNase-free DNase set, QIAGEN) according to the manufacturer’s instructions. The quality of RNA samples was determined using a 2100 Bioanalyzer (Agilent Technologies). For each sample, at least 20 µg of total RNA was sent to Beijing Genomics Institute for Illumina sequencing (commercial service). From each condition, two biological replicates were used for RNA sequencing. Each biological replicate was obtained by isolating RNA from the roots of three plants. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM1903807