GSM2584756: FG WGBS; Homo sapiens; Bisulfite-Seq
Genomic DNA was isolated using Qiagen DNeasy Blood and Tissue kit. Purified genomic DNA was sonicated into ~300 bp using Covaris focused ultrasonicators following manufacture’s instruction. Sheared DNA was ligated with methylated adaptors using a TruSeq DNA library preparation kit (Illumina) followed by sodium bisulfite treatment (Zymo Research, EZ DNA methylation-lightning kit). Whole genome-wide bisulfite sequencing (WGBS) were performed by following previous publication (18). DNA libraries with methylated adapters were amplified using KAPA HiFi Uracil+ (Kapa Biosystems) polymerase   with   4   PCR   cycles. Amplified DNA fragments were purified by AmpuXP beads and sequenced using illumina NextSeq instrument (150 cycle, pair-ended).
GEO Accession
GSM2584756