GSM4504851: primary cortical neurons, SYNCRIP - rep1; Rattus norvegicus; RIP-Seq
Cell pellet was resuspended in lysis buffer (10 mM Tris-HCl (pH=7.5), 100 mM NaCl, 0.25 % Igepal CA630, 1 mM EDTA, 1x Complete™ Protease Inhibitor Cocktail), incubated on ice for 10 min and homogenized with 20 stokes in a teflon-coated Dounce-Potter. iCLIP libraries were constructed based on Sutandy et al.,  2016
GEO Accession
GSM4504851