GSM2419769: WTTreated2; Mus musculus; RNA-Seq
mirVana miRNA Isolation Kit (Ambion) was used for total RNA extraction from mTEC 3.10 cell line (WT or Aire knockout before or after co-culture with thymocytes) according to the manufacturer's instructions. The RNA samples were eluted in RNAse free water and stored in a -80 C freezer. The yield was determined by spectrophotometry (A260) and analyzed for phenol or protein contamination, (A260/A230) and (A260/A280), respectively. The integrity of RNA samples was evaluated by microfluidic electrophoresis using Agilent RNA Nano 6000 chips and an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). (Parameters: Extracted product = total_RNA, Amplification = none) RNA libraries were prepared for sequencing using standard Illumina protocol to the TruSeq Stranded mRNA Library Preparation kit (RS-122-2101).
GEO Accession
GSM2419769