Transcriptome imformation of Nannochloropsis under phosphate deprivation
The mRNA was purified from 3~5ug total RNA, using poly-T oligo-attached magnetic beads to enrich them, then broken into short fragments randomly with the buffer to replicate the first-strand cDNA, after, second-strand cDNA. PCR was carried out with Phusion High Fidelity DNA polymerase using universal PCR primers and index primer. The library cDNA was then enriched by PCR. After cluster generation, the library preparations were sequenced on an Illumina platform and paired-end reads were generated by Biomarker Technology Corporation