Illumina HiSeq 3000 paired end sequencing; Pig transcriptome profiling in cd8 cells by the FAANG pilot project FR-AgENCODE
Paired-end RNA-Seq of polyA+ transcripts from cd8 cells on an Illumina HiSeq 3000
TruSeq Stranded mRNA Sample Preparation kit V2 Guide (with a reduction of the fragmentation time from 8 to 2 minutes)
assay type
transcription profiling by high throughput sequencing
sample storage
frozen, -70 freezer
sample storage processing
cryopreservation, other
sampling to preparation interval
21
experimental protocol
ftp://ftp.faang.ebi.ac.uk/ftp/protocols/assays/INRA_SOP_mRNA-seq_long_protocol_20160908.pdf
extraction protocol
ftp://ftp.faang.ebi.ac.uk/ftp/protocols/assays/INRA_SOP_RNA_extraction_20160504.pdf
library preparation location
Plateforme Genomique de Toulouse, INRA Castanet-Tolosan, France
library preparation location longitude
1.499654
library preparation location latitude
43.527571
library preparation date
2016-08-12T00:00:00
sequencing location
Plateforme Genomique de Toulouse, INRA Castanet-Tolosan, France
sequencing location longitude
1.499654
sequencing location latitude
43.527571
sequencing date
2016-08-12T00:00:00
experiment target
polyA RNA
rna preparation 3' adapter ligation protocol
ftp://ftp.faang.ebi.ac.uk/ftp/protocols/assays/INRA_SOP_mRNA-seq_long_protocol_20160908.pdf
rna preparation 5' adapter ligation protocol
ftp://ftp.faang.ebi.ac.uk/ftp/protocols/assays/INRA_SOP_mRNA-seq_long_protocol_20160908.pdf
library generation pcr product isolation protocol
ftp://ftp.faang.ebi.ac.uk/ftp/protocols/assays/INRA_SOP_mRNA-seq_long_protocol_20160908.pdf
preparation reverse transcription protocol
ftp://ftp.faang.ebi.ac.uk/ftp/protocols/assays/INRA_SOP_mRNA-seq_long_protocol_20160908.pdf
library generation protocol
ftp://ftp.faang.ebi.ac.uk/ftp/protocols/assays/INRA_SOP_mRNA-seq_long_protocol_20160908.pdf
read strand
mate 2 sense
rna integrity number
7.7