RNA sequence of house cricket flight muscle
mRNA was enriched from cricket flight muscle total RNA using oligo(dT) beads then fragmented and subjected to reverse transciptase.  After first-strand synthesis, a custom second-strand synthesis buffer (Illumina) was added, with dNTPs, RNase H and Escherichia coli polymerase I to generate the second strand by nick-translation and AMPure XP beads was used to purify the cDNA. Final cDNA library preparation included purification, terminal repair, Atailing, ligation of sequencing adapters, size selection and PCR enrichment (Novogene Corporation, USA).