IonTorrent  sequencing of Ceratocystis progeny
The isolates were sequenced with a low coverage whole genome sequencing approach, using Ion Torrent sequencing technology. Library building for each DNA sample was done with the Ion Xpress Plus kit using the Ion Xpress Plus gDNA fragment library preparation protocol (Thermo Fisher Scientific, Carlsbad, USA). Size selection was done using a 2% E-gel (Invitrogen). Ion Express Barcode adaptors 1-19 (Thermo Fisher Scientific, Carlsbad, USA) were used to uniquely label the DNA of different individuals, multiplexed on a single chip. Template amplification for the Ion Proton was done using the Ion OneTouch 2 System (OT2) Ion PI Hi-Q OT2 200 Kit (Thermo Fisher Scientific, Carlsbad, USA). DNA sequencing was performed on the Ion Proton apparatus