DNA-Seq of Sphenodon punctatus: adult male blood: 2x100 PE 180 insert
Total genomic DNA was extracted using proteinase K digestion and Phenol-Chloroform extraction from blood. Sequencing was undertaken using the Illumina HiSeq 2000 sequencing platform (Illumina, San Diego, CA, USA). Sequencing libraries consisted of paired end (PE) libraries with estimated insert size of 180 bp. The paired end libraries were prepared using the Illumina TruSeq PCR-Free DNA library kit as per manufacturers instructions. These libraries were normalised and pooled across 11 lanes of Illumina HiSeq 2000 using 2 x 100 bp paired end sequencing at New Zealand Genomics Ltd., Dunedin.