RNA sequencing of Nephromyces-enriched renal sac of Molgula occidentalis
Total RNA was isolated from the inside contents of a dissected renal sac of a single individual of M. occidentalis (which has been combined with RNAlater reagent; ThermoFisher Scientific), using the TRIzol reagent (ThermoFisher Scientific). A cDNA sequencing library was made using the TruSeq RNA Sample Prep Kit v2 (Illumina). The cDNA library was sequenced (2x300 bp) in a local Illumina MiSeq instrument (Centre for Comparative Genomic and Evolutionary Bioinformatics, Dalhousie University).