DNA-seq of PCa cell line: LNCaP-abl
The whole genome-amplified DNA was sheared by enzymatic fragmentation and the fragmentation time has been optimized to 20 mins at 37°C to generate the mode fragment length about 200-300 bp. End repair, A-tailing, and adapter ligation were carried out, and dual-SPRI size selection was then used to ensure the library insert size uniform. Following 5 cycles of library amplification, final libraries were purified using SPRI beads and quantified with Qubit and Agilent 2100 Bioanalyzer.