GSM3391749: mut3A.RNA; Saccharomyces cerevisiae; RNA-Seq
RNA: yeast were harvested and flash frozen. RNA was extracted using MasterPure RNA extraction kit (MPY03100). DNA: yeast cultures were collected and fixed in formaldehyde, quenched in glycine and flash frozen. Cells were lysed by beat-beating and the lysate sonicated. Lysates were incubated with the appropriate antibody for 2 hours. DNA was eluted and the crosslinks reversed. DNA was treated with RNaseA, proteinase K and purified. RNA: 1 µg of RNA was used with the NEB Ultra Directional kit (E7420) using standard protocols. ChIP-seq: 0.5 ng of DNA was used with the NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645)
GEO Accession
GSM3391749