GSM2151919: RNA Pol2-siCtrl; Homo sapiens; ChIP-Seq
Chromatin from 5 milions fixed cells was fragmented to a size range of 100–300 bases with a Diagenode Bioruptor. Solubilized chromatin was immunoprecipitated with antibody against H3K27ac, H3K4m3e, H3K27me3 and  Polimerase-II. Antibody–chromatin complexes were pulled-down using magnetic beads, washed and then eluted. After cross-link reversal and proteinase K treatment, immunoprecipitated DNA was extracted with phenol-chloroform, ethanol precipitated, and treated with RNase. ChIPed DNA was quantified by fluorometry on the Qubit system (Invitrogen). A total of 10 ng DNA were used for library preparation using NEBNext® ChIP-Seq Library Prep Reagent Set for Illumina, as recommended by the manufacturer. NEBNext ChIP-seq Library kit (New England Biolabs)
GEO Accession
GSM2151919