 whole genome sequence data for SardiNIA individual 23925 
DNA is quantitated using pico green assay.  500 ng DNA is sheared to 400 bp using Covaris sonication and loaded onto an Apollo IntegenX robot.  The DNA is end repaired, bead purified, poly-A tailed, bead purified again and adapters are ligated using IntegenX reagents.  The libraries are then bead size selected to 450 bp on the robot and amplified with 10 cycles of PCR.  Libraries are QC'd using the Agilent Bioanalyzer.  Equimolar pools are made and quantified using qPCR (Kapa Biosystems kit).  Flow cells are clustered using the Illumina cBot and sequenced on Illumina HiSeq 2000.