MERS CoV Transcriptome - Homo Sapiens Epidemic
Goals: To define known and unknown virus-specific, virus-encoded transcripts present during infection with various coronaviruses. Setup and Design: Vero cells were infected with MERS-CoV for 24 hours. Intracellular RNA was then harvested in TRIzol. RNA purified from TRIzol preps was subjected to RT-PCR and poly-A purification before generation of first - and second-strand- cDNA and Illumina HiSeq analysis