NextSeq 500 sequencing; ChIP-seq of ZMYM2-null and WT human ESCs
ChIP-seq of ZMYM2-null and WT human ESCs
washed and trypsinized phenol-chlorophorm extraction Chromatin Immunoprecipitation assay was performed as previously described (Hezroni et al. 2011; Alajem et al. 2015) with minor modifications: samples were sonicated 10 times for 30 seconds with a 30 second break between each sonication (20 minutes total). For immunoprecipitation antibodies against histone-3 pan acetylation (Millipore 06-599) and histone-3 Lysin-4 monomethylation (Millipore 07-436) were used. subsequent library preparation for sequencing was performed as previously described(Blecher-Gonen et al. 2013; Schlesinger et al. 2017)�
Experimental Factor: genotype
wild type genotype