Male
16s rRNA v3-v4 region was amplified using primers 319F (CTCCTACGGGAGGCAGCAGT) and 806R (GGACTACHVGGGTWTCTAAT) following the method by Caporaso et al. 2012.  The library was sequenced on MiSeq for 2X300bp.  The library prep and sequencing were performed by the JHMI Deep Sequencing and Microarray Core.