GSM2582323: Glc/Xyl Generalist_xyl; Escherichia coli str. K-12 substr. MG1655; RNA-Seq
Cells were harvested using the Qiagen RNA-protect bacteria reagent according to the manufacturer’s specifications. Prior to RNA extraction, pelleted cells were stored at -80°C, then thawed and incubated with lysozyme, SuperaseIn, protease K, and 20% sodium dodecyl sulfate for 20 min at 37°C. Total RNA was isolated and purified using Qiagen’s RNeasy mini kit column according to the manufacturer’s specifications. Ribosomal RNA (rRNA) was removed using Epicentre’s Ribo-Zero rRNA removal kit for Gram-negative bacteria. A KAPA Stranded RNA-Seq Kit was used to generate paired-end, strand-specific RNA sequencing libraries.
GEO Accession
GSM2582323