GSM3554954: Chromium10x_Tet2KO_2_4wk; Mus musculus; RNA-Seq
Bone marrow (BM) cells were isolated into cold phosphate-buffered saline (PBS), without Ca2+ and Mg2+, and supplemented with 2% bovine serum albumin (BSA) to generate single cell suspensions. Red blood cells (RBCs) were removed using ammonium chloride-potassium bicarbonate (ACK) lysis buffer, resuspended in PBS/2% BSA, and filtered through a 40μm cell strainer. Cells were sorted and inmediately underwent Chromium 10X protocol. For Chromium samples, libraries were prepared according to the Chromium Single Cell 3' Reagent Kits v2 User Guide.
GEO Accession
GSM3554954