GSM2766523: L1 rep2; Arabidopsis thaliana; ncRNA-Seq
Small RNAs were extracted from whole plants by Trizol reagent (Invitrogen), resolved on a 15% denaturing PAGE gel, and visualized by SYBR-Gold (Invitrogen) staining. Gel slices within the range of 18-30 nt were excised, and the RNAs were eluted and purified for small RNA library construction. For whole-genome bisulfite sequencing, genomic DNA was isolated using a DNeasy Plant kit (Qiagen) according to the manufacturer’s instructions. For small RNA deep sequencing, small RNAs were extracted by Trizol reagent (Invitrogen), resolved on a 15% denaturing PAGE gel, and visualized by SYBR-Gold (Invitrogen) staining. Gel slices within the range of 18-30 nt were excised, and the RNAs were eluted and purified for small RNA library construction. For whole-genome bisulfite sequencing, the libraries were constructed using an Accel-NGS® Methyl-Seq DNA Library Kit (Swift Biosciences). Small RNA libraries were constructed as described(Mi et al., 2008)
GEO Accession
GSM2766523