Whole genome sequencing of Histoplasma capsulatum SG1 yeast-locked mutant strain
The SG1 mutant and the control strain were grown up from frozen glycerol stocks onto HMM agarose plates supplemented with uracil. Liquid cultures were started for each strain and passaged twice at 1:25 dilution in HMM+uracil. Two days after the second passage the strains were collected via centrifugation. Genomic DNA of the strains was isolated using the Gentra Puregene Yeast Kit (Qiagen). Libraries were made using the Nextera XT DNA Library Prep Kit (Illumina).