Illumina Genome Analyzer II paired end sequencing; Whole transcriptome profiling of the vernalization process in Lilium longiflorum bulbs
Whole transcriptome profiling of the vernalization process in Lilium longiflorum bulbs
L. longiflorum bulbs (cultivar White Heaven) were obtained from a nursery at the end of the growing season (August). Bulbs were sanitized and stored in humid standard pot medium at 25C (control, 0W) or at 4C for 2, 5, 7 or 9 weeks (2W, 5W, 7W, 9W, respectively). At each of these time points, shoot apical meristems were excised from the bulbs, immediately frozen in liquid nitrogen and then stored at -80C until RNA extraction. RNA was isolated using the AurumTM Total RNA mini kit (Biorad) following manufacturers instructions. The procedure was followed by an additional DNase treatment with TURBO DNase (Life technologies) to remove all genomic DNA still present after the isolation. The RNA integrity was confirmed using the 2100 Bioanalyzer (Agilent Technologies). The samples were prepared for transcriptome sequencing using the Illumina kit (TruSeq RNA Sample Preparation Kit v2) following the manufacturers recommendations.
Experimental Factor: growth condition
Vernalized for 5 weeks at 4C