GSM2151587: Th0 72h aIL4/4R H3K36Me3 ChIP-seq; Mus musculus; ChIP-Seq
RNA was harvested using BioRad total RNA extraction kit with DNaseI treatment. For ChIP cells were fixed with 0.8% formaldehyde for 8 min at RT and DNA was processed and sonicated according to the protocol from Covaris using Covaris S220 sonicator for 4-5 min. RNA  libraries for pair-end read sequencing were prepared by using standard Illumina protocols. ChIP libraries were prepared by using ThruPLEX DNA kit (Rubicon).
GEO Accession
GSM2151587