Transcriptomic analysis of differentially expressed genes relevant to tepals color fading in Lycoris longituba
Twelve cDNA libraries which consisted of separate RNA samples from L. longituba Pink tepals of three different developmental stages (S1-P, S2-P and S3-P) and L. longituba White tepals (S3-W) were prepared using the TruSeq RNA Sample Preparation Kit (Illumina, USA) according to the manufacturers instructions. Firstly, the enriched mRNA were fragmented into short fragments and reverse transcripted into first-strand cDNA. Then after second-strand cDNA synthesize, end repair, adapter ligation, and PCR amplification, the cDNA library products were sequenced on Illumina HiSeqTM 4000 instrument using paired-end sequencing technology by staff at Gene Denovo Biotechnology Corporation (Guangzhou, China). After the the raw reads removing the adapter sequences, reads with more than 20% low-quality bases (quality value<20) and ambiguous nucleotides (denoted with anNin the sequence trace) of the raw reads, the high-quality clean reads were accomplished assembled using Trinity software. In order to avoid the interference of alternative splicing transcripts, only the longest transcript was taken as the unigene in this research.