GSM3560346: cldn15la-GFP(+) f-Dex(+) LREs 1; Danio rerio; RNA-Seq
At 6 dpf (24 hrs post gavage), zebrafish larvae were dissociated using Trypsin/EDTA and collagenase. Then two different intestinal populations were collected using fluorescence-activated cell sorting (FACS). Total RNA was prepared from each population using RNeasy Micro Plus Kit (Qiagen). Quality control was evaluated by Qubit and 2100 Bioanalyzer. Clonetech ultra low input RNA libraries were prepared for sequencing using standard Illumina protocols.
GEO Accession
GSM3560346