GSM2152263: MRC-5; Homo sapiens; RNA-Seq
RNA was isolated from siCTRL and siNSUN2 HeLa cells and 3T3-L1 cells, mutiple human other cell lines, mouse other cell lines and tissues using the TRIzol (Invitrogen) reagent by following the company manual. For all samples the RNA integrity was checked using an Agilent Bioanalyzer 2100. Approximately 2.5 µg of total RNA was then used for library preparation using a TruSeq™ RNA Sample Prep Kit v2 (Illumina, San Diego, CA, USA) according to the manufacturer’s protocol.The libraries were sequenced using HiSeq2000 or HiSeq3000 (Illumina) in paired-read mode, creating reads with a length of 101 bp.
GEO Accession
GSM2152263