C3xAlt_POOL5
Pooled multiplex fragment libraries of 23 individuals from a segregating F1 population generated from the cross of globe artichoke "C3" genotype and cultivated cardoon "Altilis 41" genotype. DNA samples were fragmented to lengths of ~300 bp by sonication using a Covaris E220 as per the manufacturer’s instructions (Covaris, Inc.). Genomic paired-end libraries were then prepared using standard procedures and NEB reagents for end-repair, A-tailing, and adapter ligation. Fragment size was controlled using Ampure beads purification procedure. Libraries were PCR-enriched using the high-fidelity Phusion polymerase with 5 cycles of amplification. These libraries were prepared with the aim to perform shallow coverage sequencing for genotype imputation purposes.  Individuals are identified with the following barcodes in the header: Art_201:ACTGAT; Art_202:ATGAGC; Art_203:ATTCCT; Art_204:CAAAAG; Art_205:CAACTA; Art_206:CACCGG; Art_207:CACGAT; Art_208:CACTCA; Art_209:CAGGCG; Art_210:CATGGC; Art_211:CATTTT; Art_212:CCAACA; Art_213:CGGAAT; Art_214:CTAGCT; Art_215:CTATAC; Art_216:CTCAGA; Art_217:GCGCTA; Art_218:TAATCG; Art_219:TACAGC; Art_220:TATAAT; Art_221:TCATTC; Art_222:TCCCGA; Art_223:TCGAAG