Bulk RNA sequencing for classically activated macrophages (CAMs) from Ninj1+/+ and Ninj1-/- mice
BM cells were isolated from the femurs and tibias of Ninj1+/+ and Ninj1-/- mice at 6 weeks of age and cultured in DMEM containing 20% FBS and 20 M -mercaptoethanol for 16 hours. The suspended cells were plated and treated with 20 ng/ml recombinant human/mouse macrophage colony-stimulating factor (M-CSF, 30ng/ml) for 7 days to differentiate macrophages. For activation in vitro, mouse differentiated macrophages were stimulated with lipopolysaccharide (LPS, 500 ng/ml) and IFN (20 ng/ml) to M(LPS/IFN)s, which were classically activated macrophages (CAMs).