GSM4191200: Control_Rep3; Mus musculus; RNA-Seq
We used CRISPR/Cas9 system to generate female Adra1a-deficient hAGT transgenic mice with a frameshift mutation that leads to a knockout of the Adra1a gene. Then, we generated PAH/KO mice by mating female Adra1a-deficient hAGT transgenic mice with male hRN transgenic mice. Whole hearts were frozen in liquid nitrogen and were crushed into powder with a Multi-beads Shocker (Yasui Kikai Co.). Total RNA was extracted using ISOGEN II reagent (Nippon Gene). 500 ng of total RNA were ribosomal RNA-depleted using NEBNext rRNA Depletion Kit (New England Biolabs), and then converted to Illumina sequencing library using NEBNext Ultra Directional RNA Library Prep Kit (New England Biolabs). Reads were mapped on GRCm38 (mm10) mouse reference genome and quantified using CLC Genomics Workbench version 12.0 (QIAGEN). RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM4191200