GSM3557794: Macrophage HDM rep1; Mus musculus; RNA-Seq
Cells were treated with 3 mg/ml collagenase solution for 45-60 min to extract them from the gels and subsequently RNA was isolated using RNeasy Mini purification kit (Qiagen) RNA quality was assessed using the Agilent 2100 Bioanalyzer. Total RNA (1000 ng) was prepared for sequencing using polydT-mediated cDNA synthesis according to the manufacturer´s (Illumina) instructions. Subsequent library preparation was performed using the NEBNext RNA library preparation kit for Illumina. Library quality was assessed using the Fragment Analyzer (AATI) followed by library quantification (Illumina library quantification kit). Sequencing was carried out on a HiSeq1500 platform (Illumina) with a read length of 50 bp.
GEO Accession
GSM3557794