GSM3868654: IP Clone b, 4OHT treated for 2hrs  [EP300]; Mus musculus; ChIP-Seq
Cells we fixed in 1% formaldehyde at RT for 10 minutes and subsequently 2mM disuccinimidyl glutarate , nuclei extracted and chromatin sonicated using a bioruptor (10 cycles, 30s on 30s off). Cleared chromatin was incubated with anti-TBP antibody (Abcam,ab14984). Input DNA serves as negative control for antibody enrichment. Libraries were prepared using the NEB Next Ultra 2 kit.