D_sechellia_R1
Please see Garrigan et al. 2012. Genomic DNA (gDNA) was extracted from 15 female flies. gDNA was also extracted from 15 female flies of the D. sechellia reference strain (14021-0248.25). gDNA was extracted using a modified Puregene DNA Extraction Protocol. The 15 frozen female flies for each library were lysed by 300 L cold Cell Lysis Solution (Qiagen, 158906) with pestle homogenization and incubation at room temperature for 15 min, and treated with 4.5 L RNase A at 37C for 15 min. Proteins were precipitated out with 100 L Protein Precipitation Solution (Qiagen, 158910) and centrifugation at 16,000g for 4 min. gDNA in supernatant was purified by DNA Clean and Concentrator 25 Kit (Zymo, D4005) with five volumes of binding buffer to sample. For the library preparations of  D. sechellia, 3 g gDNA was sheared to 300 bp with Covaris, followed with end-repairing, A-tailing, ligating to adaptors, selecting size between 300 and 450 bp with 2% agarose gel, and PCR amplifying for 15 cycles. The final product size was estimated to be between 400 and 500 bp on a 2% agarose gel.