GSM4628262: PFC_CTRL_rep1; Mus musculus; RNA-Seq
For each sample, the brain was extracted after rapid anesthesia induction with 5% isoflurane. The vmPFC was manually excised and the cells dissociated according to a published protocol (Saxena et al., 2012, Biotechniques). Cell viability was measured with Trypan blue and resuspended in ice-cold PBS to desired concentrations for 10X Genomics single cell capture. Sequencing libraries were prepared using the 10X Genomics 3' single cell gene expression V2 kit following the manufacturer's protocol
GEO Accession
GSM4628262