GSM2838590: DDX3X_mRG_3; Homo sapiens; RNA-Seq
RNA extracted using TRI® reagent (Sigma-Aldrich) as per manufacturer's instructions. iCLIP performed as in Huppertz et al., Methods, 2014 with changes allowing cytoplasmic RNP isolation (Herdy et al., Nat. Immunol, 2012). RNA-seq libraries were generated using the Illumina TruSeq Stranded total RNA kit (Illumina, cat #RS-122-2301) as per the manufacturer's instructions. iCLIP libraries prepared as in Huppertz et al., Methods, 2014 with RT buffer allowing efficient quadruplex reverse transcription (Kwok et al., Nat. Methods, 2016). Sequenced using an Illumina NextSeq 500.
GEO Accession
GSM2838590