circRNA-Seq of mus musculus: adult female brain
The total RNA was treated with Ribo-ZeroTM rRNA Removal Kit (Illumina, San Diego, CA, USA) and RNase R to remove rRNA and linear RNA. The remaining RNA was fragmented and then reversely transcribed with random hexamer primers. The cDNA library containing 300~400 bp fragments was constructed by the TruSeq RNA Sample Prep Kit (Illumina) and sequenced on the Illumina HiSeq4000 sequencer (Illumina) in 150bp paired-end reads