GSM4192443: 6C2 cells, cell sample s2, Total RNAseq; Gallus gallus; RNA-Seq
RNA from 6C2 cells were isolated using RNeasy plus mini kit (Qiagen) following manufacturer's instruction. DNase digestion was performed (Promega) to remove any genomic DNA in the purified RNA. SOLiD Total RNA-Seq kit was used to prepare whole transcriptome libraries. RNA quality and integrity was assessed using the RNA pico Kit and 2000 Bioanalyzer  (Agilent technologies). 4-6 ug of Total RNA was subjected to rRibosomal depletion (RiboMinus eukaryote system v2, Life technologies) followed by RNAse III fragmentation, adaptor hybridization and reverse transcription. Reversed transcribed DNA was size selected and PCR amplified. Libraries were sequenced in a multiplex manner, pooling two libraries per lane.
GEO Accession
GSM4192443