GSM2768188: RNA-Seq single cell_CK_1w_m3_G7; Mus musculus; RNA-Seq
Hippocampal tissue was enzymatically digested using the Neural Tissue Dissociation Kit (P) (Miltenyi Biotec, catalogue number 130-092-628) according to the manufacturer's protocol. FACS sorted single cell lysis in 96-well plates were subject to Smart-seq2 RNA library preparation. A QC step was performed to check amplification of the libraries before multiplexing for sequencing. RNA from single cells was first purified with Agencourt RNAClean XP beads (Beckman Coulter, catalogue number A63987) before oligo-dT primed reverse transcription with Maxima H Minus Reverse Transcriptase (Thermo Fisher, catalogue number EP0753), which was followed by 21 cycle PCR amplification using KAPA HiFi HotStart ReadyMix (KAPA Biosystems, catalogue number KK2602). Libraries were tagmented using the Nextera XT DNA Library Preparation Kit (Illumina, catalogue number FC-131-1096) and the Nextera XT Index Kit v2 Sets A,B,C, and D according to the manufacturer's instructions with minor modifications.
GEO Accession
GSM2768188