GSM4629054: Kuramochi Input for CTCF; Homo sapiens; ChIP-Seq
CTCF ChIP-Seq was performed as previously described (Schmidt et al., Methods, 2009) using 25 μg chromatin sheared to 100-300 bp fragments using a Covaris E220 evolution Focused Ultrasonicator. ChIP libraries were constructed using the Kapa Hyper Library Preparation kit, quantified and sequenced on an Illumina NextSeq 500 sequencer. Whole cell extracts were sonicated to solubilize the chromatin. The chromatin extracts containing DNA fragments with an average size of 500 bp were immunoprecipitated using different antibodies.
GEO Accession
GSM4629054