ChIPexo of U-2 OS tetracycline NRF2 doxycycline treated
Upon ~95% cell confluence, DNA-protein complexes were cross-linked with formaldehyde. Cells were washed with PBS and suspended in SDS lysis buffer. Samples were sonicated. The solubilized chromatin was diluted with ChIP dilution buffer (1:9 ratio) for ChIP assays.  The chromatin was pre-cleared and then incubated with anti-FLAG antibody,or mouse normal IgG overnight at 4 C with rotation. Cross-linked immunoprecipitates and total chromatin input were reverse cross-linked respectively and the DNA was isolated. The library was created using adapter ligation methods, and each library was sequenced along with an input control library on HiSeq3000 Illumina Sequencing Platform as 50 bp single-end read runs.