GSM2584476: R540Q PFV (codon-optimized, 4-component) vector in HepG2 cells; Homo sapiens; OTHER
Cellular genomic DNA isolated 5 days post-infection was digested with BfaI/BanII/CviQI and ligated to a linker made by annealing oligonucleotides 5´- TACCGGTCAGCATAGTG (with 3´ end blocked by an amino group modifier, and 5´-phosphorylated) and 5´-GACTTGAACCGTAGCATCTAAGCACTATGCTGACCGG. PFV integration sites were amplified using primers 5´- CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCTGACTTGAACCGTAGCATCTAAG and 5´-GTGTGAA CTACACTTATCTTAAATGATG followed by a nested PCR with primers 5´- CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCTGACTTGAACCGTAGCATCTAAG and 5´- AATGATACGGCGACCACCGA GATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTXXXXXCTTAAATGATGTAACTCCTTAGGATAATCAATATAC (the string of underlined nucleotides marked with “X” denotes the position of a 5-base index, which was unique for each integration site library analyzed). 
GEO Accession
GSM2584476