Genomic SMRT sequencing of X. oryzae pv. leersiae BB156-2
Long read, single molecule real time sequencing (SMRT, PacBio, Menlo Park, CA) data was generated for five Xol strains and for the Xo strain X11-5A, to be used as an outgroup. DNA for SMRT sequencing was isolated by culturing strains on nutrient agar for 48 h then using the Genomic DNA buffer set and Genomic-tips according manufacturer instructions (Qiagen, Valencia, CA). SMRT sequence was assembled using HGAP v4 (PacBio, Menlo Park, CA). Genomes were circularized using circulator (Hunt et al., 2015).