GSM3098135: WT-NBAF rep1; Geobacter sulfurreducens PCA; RNA-Seq
For each biological sample, total RNA samples were extracted using the RNeasy mini kit (Qiagen), then they were examined with an Agilent 2100 Bioanalyzer and quantified using NanoDrop 200c (Thermo Scientific). Illumina sequencing was performed at the UUSMD (UNAM, Mexico). After removing residual DNA using DNase I (ThermoScientific) and ribosomal RNA with Terminator 5´-Phosphate-dependent exonuclease (Epicentre), the mRNA-enriched RNA was chemically fragmented to 150~200 bp. Based on these cleaved RNA fragments, cDNA was synthesized using a random hexamer primer and reverse transcriptase. After end reparation and ligation of adaptors, the products were amplified by PCR, further purified, and used to create the final cDNA library. Libraries were sequenced on an Illumina Genome Analyzer IIx.
GEO Accession
GSM3098135