amplicon from tapirus fecal sample
Total DNA was extracted from fecal samples using the DNeasy Blood & Tissue kit (Qiagen) DNA samples were PCR amplified using the V4 hypervariable region of the 16S rDNA gene with universal bacterial/archaeal primers F515 (5-GTGCCAGCMGCCGCGGTAA-3) and R806 (5-GGACTACHVGGGTWTCTAAT3) Caporaso et al (2011). PCR products were pooled and purified with Ampliclean carboxyl-coated magnetic beads (NimaGen, NDL). 20 ng/l sample were used to construct the library which was sequenced on an Illumina MiSeq 2 x 300 platform (Yale Center for Genome Analysis, CT, USA).