GSM3174278: S-14: Skin; Mus musculus; RNA-Seq
Back skin was unhaired and digested with elastase(0.2mg/ml) in DMEM medium for 20min at 37°C, then the tissue was minced and digested with type Ⅳ collagenase (4 mg/ml) in DMEM medium for 1.5h at 37°C. Digested tissue was successively filtered through 70-μm and 40-μm cell strainers (BD Biosciences) and then centrifuged with 500g for 5min. The cell pellet was resuspended with PBS. Tomato+ cells were sorted by Bio-Rad S3e and then single cells were picked with fluorescence microscope. Libraries were prepared according to Nextera® XT DNA Library Prep Kit for Illumina  (Cat# FC-131-1096) RNA-seq (SMART-Seq v4 3' DE Kit)
GEO Accession
GSM3174278