GSM4681391: CBA_26M_SV_C_L002; Mus musculus; RNA-Seq
Cells were separately suspended in RNALater (~100 µL in total volume) and total RNA, including small RNAs (> ~18 nucleotides), were extracted and purified using the Qiagen RNeasy mini plus Kit (Qiagen, Germantown, MD). On-column DNase digestion was performed to eliminate DNA contamination in the collected RNA. Libraries were prepared using the SMARTer Low Input RNA kit (Clontech Laboratories, Mountain View, CA) to generate cDNA in combination with the Nextera Library preparation kit (Illumina, San Diego, CA). Library size and concentration were assessed using an Agilent 2100 Bioanalyzer and a Quibit fluorometer (Invitrogen, Thermo Fisher Scientific) to ensure the inserts were the appropriate size and determine concentration prior to sequencing. Transcriptome libraries were sequenced using the HiSeq 2500 Sequencing System (Illumina).
GEO Accession
GSM4681391