GSM3938916: RNA_Bcl6KO ST0_3; Mus musculus; RNA-Seq
Single-cell thymocyte suspensions were acquired with mechanical dissociation and cells were stained with appropriate antibodies and tetramers. Samples were flow-sorted and cells were frozen in RLT buffer, for RNA extraction. RNA was extracted with QIAGEN microRNAeasy kit (Cat No: 74004). NuGEN Ovation Ultralow Library systems (NuGEN Technologies)