isoseq data of Luffa Acutangula
Total RNA was extracted from leaf and root tissues using the Concert Plant RNA Reagent (Invitrogen, Carlsbad, USA). Poly(A) mRNAs were enriched from total RNA samples using the Absolutely mRNA Purification Kit (Agilent Technologies, Santa Clara, USA). The RNA integrity was assessed with a Bioanalyzer (Agilent Technologies, Santa Clara, USA) prior to the con- struction of the Iso-Seq library. We prepared the Iso-Seq library according to Pacific Biosciences Iso-Seq protocol using the SMARTer PCR cDNA Synthesis Kit (Clontech, Mountain View, USA) and the BluePippin Size Selection System (Sage Science, Beverly, USA). cDNA libraries were selected for the following bins: 12 kb, 23 kb, and 36 kb and sequenced on a PacBio RS II instrument using P4-C6 polymerase and chemistry with 360 min movie times.