WA7733_38SMRT_PB
High molecular weight (HMW) genomic DNA was isolated for PacBio and Illumina sequencing. HMW gDNA was isolated using a modified nuclei preparation followed by phenol chloroform purification to remove residual contaminants. PacBio libraries size selected for 25kb fragments on the BluePippen system (Sage Science) and purified using AMPure XP beads (Beckman Coulter). The 25kb PacBio libraries were sequenced on a PacBio RSII system with P6C4 chemistry