GSM2759015: HeLa_Control_AMO_4hr_Labeled_rRNA_depletion; Homo sapiens; RNA-Seq
Total RNA extracted using trizol, total mRNA extracted using Ribozero kit, nascent RNA linked to Biotin and etracted on magnetic streptavidin beads Libraries were prepared according to instructions accompanying the KAPA kit (#KK8400). Briefly, RNA was fragmented using MgCl2+ and heat, then converted into cDNA using a strand-specific synthesis method with dUTP. cDNA was A-tailed, and adapters were ligated. The library was then amplified, and any adapter dimers were removed with SPRI beads.
GEO Accession
GSM2759015