RNAseq of U-2 OS tetracycline NRF2 doxycycline treated replicate 2
Total RNA isolated using Trizol and a column-based RNeasy kit (Qiagen). Ribosomal RNA was depleted using Ribo-Zero Gold rRNA Removal kit (Illumina), and prepared for sequencing using the NEB Ultra Directional RNA library prep kit for Illumina (NEB). Samples were run on HiSeq3000 Illumina Sequencing Platform as 50 bp single-end read runs.