Comparative transcriptome analysis of salicylic acid regulated high temperature stress in Ulva prolifera
U. prolifera was treated with 0.1 mmol/L salicylic acid and transferred to a high temperature 35 °C growth chamber with the same irradiance and photoperiod. In addition, U. prolifera were cultured at 35 °C without salicylic acid treatment as the control. The gametophyte thalli were harvested 1 and 3 h after transfer to the 35 °C growth chamber. The gametophyte thalli were snap-frozen with liquid nitrogen and maintained at -80 °C until RNA extraction.Total RNAs isolated from the gametophyte thalli, which were harvested 1 and 3 h were mixed with equal and used to extraction. These RNAs were used to construct cDNA library.