P493-6 cell line, dox-treated, environmental stimuli
Cell pellets (Mix of P493-6 cells and Drosophila melanogaster spike-in cells) were lysed and total RNA extracted using the NucleoSpin RNA Isolation Kit (Machery-Nagel) according to the manufacturer's protocol. Total RNA was spiked with a 1:100 dilution of ERCC spike-in Mix 1 (Life Technologies). Library preparation: TruSeq RNA Sample Preparation Kit v2 (Illumina).