RNA-seq of Mus musculus: Th2 Early Gata3 KO Rep 1
Total RNA was extracted with RNAeasy Mini Kit (QIAGEN, 74104). 10 ng RNA was amplified with the Ovation RNA-Seq System V2 (7102-08, NuGEN) per manufacture’s instruction. 220 ng double stranded DNA was sonicated into 200–400 bp using Diagenode Bioruptor. cDNA was blunt-ended, ligated to Illumina adaptors, amplified, and sequenced with Illumina HiSeq system.