Illumina HiSeq 2000 sequencing; qiita_ptid_83:804.H04.072805.R0325
We are now requesting sequencing of additional samples collected from carbonate chimneys at the Lost City hydrothermal field, located near the Mid-Atlantic Ridge at a depth of ~800 m. We have previously shown that extreme (anoxic, pH 9-11, high H2) Lost City chimneys host bacterial communities with strong taxonomic and functional similarities to those of the Newfoundland and Liguria springs mentioned above (Brazelton et al., 2012), but an exhaustive census of these Lost City chimneys has yet to be performed.
EMP V4 515f,806rbc protocol
barcode
ACTAGTTGGACC
center_name
CCME
center_project_name
Brazelton_LostCity_chimney_biofilm
emp_status
EMP
illumina_technology
HiSeq
linker
GT
pcr_primers
FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
primer
GTGTGCCAGCMGCCGCGGTAA
run_center
CCME
run_date
3/1/14
run_prefix
Brazelton_LostCity_chimney_boifilm_lane4_NoIndex_L004
samp_size
0.1, g
sample_center
East Carolina University
sequencing_meth
Sequencing by synthesis
study_center
CCME
target_gene
16S rRNA
target_subfragment
V4