WGS Spontanouts RBT Mutant
Sequencing libraries for the selected mutants and parent strain were prepared at the USC Molecular Genomics Core. Libraries were simultaneously prepared from extracted genomic DNA using the Illumina Nextera XT library prep kit according to the manufacturers protocol (Cat#FC-131-1024, Illumina). Prepared libraries were sequenced on the Illumina Miseq V2 at 2x150 cycles. Assembled reads were aligned to the previously published A. baumannii HUMC1 sequence (NCBI Reference Sequence: NZ_LQRQ01000007.1)