Amplicon-Seq of xoxF4: Elba methane SIP T2 sediment replicate A DNA
The xoxF4 gene encoding a methanol dehydrogenase was amplified using primer pair xoxF4f/r as described in Taubert et al. 2015, EM, 17(10):3937-48. Triplicate PCR products were combined and purified using 10 g of linear polyacrylamide and 270 l of polyethylene glycol-NaCl 6000 solution. Purified amplicons were subjected to 454 pyrosequencing (GS FLX Titanium system, MR DNA, Shallowater, TX, USA).