GSM1903777: CKO1; Mus musculus; RNA-Seq
Fully-grown GV oocytes were obtained from the ovaries of 4-6-week-old female mice 48 hours after intraperitoneal injection of 5 IU of pregnant mare’s serum gonadotrophin. GV oocytes were released by puncturing antral follicles with a fine needle on the stage of a dissecting microscope. Each sample, which contained 100 oocytes, was lysed directly in 1 μl of prelude direct lysis buffer (Nugen). RNA was subject to amplification using the ovation RNA-seq system v2 (Nugen). Amplified cDNA was fragmented using Covaris and paired-end libraries were then constructed using the TruSeq RNA Sample Preparation Kit v2 (Illumina) starting from end repair.
GEO Accession
GSM1903777