Methanotrophic community composition of permaforst thaw ponds in subarctic Quebec: Sample S1ASS_2013_pmoA
RNA was extracted using the AllPrep DNA/RNA Mini Kit (Qiagen). mRNA of the pmoA converted to cDNA were PCR amplified using the dual-indexed forward 169 (5'-GGNGACTGGGACTTCTGG-3') and reverse primers 661 (5'-CCGGMGCAACGTCYTTACC-3'). The resulting 40 Biosamples were sequenced using illumina MiSeq technology.