GSM3325685: Flag-wt-FUS replicate 2; Homo sapiens; OTHER
Cell pellets were lysed in in NP40 lysis buffer (50 mM HEPES-KOH, 150 mM KCl, 2 mM EDTA, 1 mM NaF, 0.5% (v/v) NP40, 0.5 mM DTT and complete EDTA-free protease inhibitor cocktail) and protein-RNA complexes precipitated using FUS antibody (Santa Cruz, sc-47711) or flag M2 antibody (Sigma, F1804). PAR-CLIP was performed as described in Maatz et al. 2017 (doi:10.1002/cpmb.35), with the exception of the 3' adapter ligation, which was performed on beads after immunoprecipitation.
GEO Accession
GSM3325685