GSM1533522: Col0 replicate 2 mRNA; Arabidopsis thaliana; RNA-Seq
Total RNA was isolated with TRIzol (Ambion) from 0.1 g of tissue following the product manual. 2 µg of total RNA were treated with DNase I, amplification grade (Invitrogen) for 15 min at room temperature. DNase I was heat inactivated with 2.5 mM EDTA at 65 ºC for 10 min. Individual libraries for each biological replicate were generated from 2 µg total RNA according to the manufacturer’s instructions (Illumina).
GEO Accession
GSM1533522