GSM2838913: B14_r2; Mus musculus; RNA-Seq
Macrophages were isolated from inert conduits 5, 14 and 28 days after sciatic transection by flow cytometry (Lin-F4/80+CD14+CD11b+CD16/32+). Total RNA was isolated with the Quick-RNA MicroPrep kit (Zymo Research). RNA-seq libraries were prepared from 25 ng total RNA using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs), with initial polyA+ isolation.
GEO Accession
GSM2838913