GSM4191795: Mcph1 KO2; Mus musculus; RNA-Seq
Total RNA was extracted from WTNPCs and WTNPCs. Sample quality and quantification were assessed on Experion chips (Bio-Rad). Twin samples were pooled according to genotype and matching amounts. Library preparation and Illumina sequencing were performed on wild type and Mcph1 KO samples, two of each, at the Genomic Platform of the Ecole Normale Superieure (Paris, France). Ribosomal RNA depletion was performed with the Ribo-Zero kit (Epicentre), using 1.5 μg of total RNA. Libraries were prepared using the strand specific RNA-Seq library preparation ScriptSeq V2 kit (Epicentre). Libraries were multiplexed by 4 on 1 flow cell lane. A 75bp read sequencing was performed on a HiSeq 1500 device (Illumina). A mean of 39.3 ± 7 million passing Illumina quality filter reads was obtained for each of the 4 samples.
GEO Accession
GSM4191795