GSM2759697: tps1_gal_fru_15m; Saccharomyces cerevisiae; RNA-Seq
total RNA was extracted using a standard yeast RNA extraction protocol (using hot, acidic phenol); briefly, cells are resuspended in a lysis buffer and acidic phenol, then incubated at 65C for 30 minutes to dissolve the proteins/membranes and release the RNA.  Next RNA is purified using alcohol precipitation. libraries were prepared using Illumina's Tru-seq stranded mRNA prep kit (poly-dT selection of polyA transcripts)(using Invitrogen's SuperScriptIV); fragments ranging from 200-600 bp were selected using a Pippin-HT
GEO Accession
GSM2759697