Lynch syndrome colon cancer tissue
Genomic DNA extracted from peripheral blood for each sample wasfragmented to an average size of 180~280bp and used to create a DNA library following establishedIllumina paired-end protocols. The Agilent SureSelect Human All ExonV6 Kit (AgilentTechnologies, Santa Clara, CA, USA ) was used for exome capture according to the manufacturersinstructions. The Illumina Novaseq 6000 platform (Illumina Inc., San Diego, CA, USA) was usedfor genomic DNA sequencing by Novogene Bioinformatics Technology Co., Ltd (Beijing, China) togenerate 150-bp paired-end reads with a minimum coverage of 10 for ~99% of the genome (meancoverage of 100).