Cow milk exosome miRNA
total RNA samples were fractionated and only small RNA ranging from 16 to 30 nt were gel purified and ligated to the 3' adaptor and 5' adaptor. Ligation products were gel purified, reverse transcribed and amplified. The purified cDNA library was used for cluster generation on Illuminas Cluster Station (San Diego, CA, USA) and subsequently sequenced on Illumina HiSeq 2500 platform (San Diego, CA, USA), following the manufacturers instruction on running the instrument.