GSM4182285: Imp::GFP RNAseq 2; Drosophila melanogaster; RNA-Seq
Brains were removed and flash frozen in liquid nitrogen before lysis. RNA was harvested using RNASpin Mini kit (GE Healthcare). polyA RNA was enriched using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) according to manufacturer's instructions. Poly(A) enriched RNA was then used for library production using the Ion Total RNA-Seq Kit v2 for Whole Transcriptome Libraries (Life Technologies). Libraries were prepared using Ion Total RNA-Seq Kit v2 for Whole Transcriptome Libraries (Life Technologies) according to manufacturer's instructions.
GEO Accession
GSM4182285