Illumina HiSeq 2500 paired end sequencing; RNA-Seq of steady-state and LPS stimulated splenic classical dendritic cells from wild type, germ-free and IFNAR knockout mice
RNA-Seq of steady-state and LPS stimulated splenic classical dendritic cells from wild type, germ-free and IFNAR knockout mice
Spenocytes were stained for CD3- CD19- CD11c+ MHC-II+ CD45+ and sorted to high purity by flow cytometry Isolated cells were plated in D10+ medium, stimulated with either PBS or LPS for 2h. The cells were collected. Total RNA was isolated by Trizol extraction Libraries were prepared by the Deep Sequencing Unit at the MPI in Freiburg, Germany. The library preparation included a polyA enrichment.
Experimental Factor: C57BL/6
strain
Experimental Factor: wild type genotype
genotype
Experimental Factor: lipopolysaccharide
stimulus