GSM3940110: H3K4me_Klf5_H3K27ac_input+KrasG12D_ChIP; Mus musculus; ChIP-Seq
Briefly, cells were crosslinked at room temperature for 10 min with 1% formaldehyde,  washed twice with PBS and sonicated in lysis buffer (50 mM HEPES/KOH pH 7.5, 140  mM NaCl, 0.1% Na-Deoxycholate, 1% Triton X-100, 1 mM EDTA, complete protease  inhibitor cocktail). Samples were immunoprecipitated at 4 ºC overnight and washed six  times with ChIP wash buffer (20 mM Tris, pH 7.9, 500 mM NaCl, 2 mM EDTA, 1% Triton  X-100, 0.1% SDS) and once with TE buffer. Bound DNA was eluted in 1% SDS buffer  and reverse-crosslinked for 6 h at 65 ºC DNA samples were treated sequentially with  RNase A and Protease K and then purified with ChIP DNA Clean & Concentrator-5  (Zymo Research, D4014)
GEO Accession
GSM3940110