GSM1633736: Starvation day20 rep1; Mycobacterium bovis BCG str. Pasteur 1173P2; RNA-Seq
Cell pellets were lysed by bead-beating in Trizol reagent (Invitrogen) and RNA purified with on-column DNaseI digest and PureLink miRNA spin columns (Ambion). The second PureLink miRNA spin column depletes tRNA. rRNA is depleted using the Ribo-ZeroTM Magnetic Kit for Bacteria (Epicentre, Illumina) as per manufacturer’s instructions. RNA libraries were prepared for sequencing using standard Illumina protocols using TruSeq® RNA Sample Preparation v2 kit (Illumina).
GEO Accession
GSM1633736