GSM2579375: Mouse D3839 mutant erythroid cells ATAC-seq biological replicate 1; Mus musculus; ATAC-seq
A single cell suspension was made by gently dissociating the spleen of a phenyhydrazine treated mouse and passing it through a 70um filter. Ter119 selection was performed by staining the cells with phycoerythrin conjugated anti-ter119 antibodies (BD biosciences). The cells were subsequently washed and then incubated with MACS anti-phycoerythrin beads. Cells were then separated using an auto-MACS (Miltenyi Biotec). Illumina Nextera DNA library kit, subjected to ATAC-seq (Assay for Transposase-Accessible Chromatin)
GEO Accession
GSM2579375