microbiota of obese and no obese horse feces
Genomic DNA was extracted using QIAamp Fast DNA Stool Mini Kit DNA was suspended in tris-EDTA buffer and stored at -80C. Amplification and sequencing of the V4 variable region 16S rRNA gene was performed by a commercial laboratory. A DNA library was prepared according to Illumina TruSeq DNA library preparation protocol. Sequencing was performed on an Illumina MiSeq.