KY07
Wing tissue samples were obtained from hibernating little brown myotis with no known exposure to Pseudogymnoascus destructans and bats exhibiting physical signs of white-nose syndrome (WNS). RNA was purified from 50 mg of wing tissue using a QIAGEN RNeasy Plus Universal Mini Kit. All samples used for RNA sequencing had RNA integrity values greater than 7.0 using an Agilent Bioanalyzer. The Genome Sequencing and Analysis Facility at the University of Texas at Austin performed all library preparation and quality control procedures. Directional RNA libraries were prepared with poly-A mRNA enrichment, dUTP/UDG strand-specific labeling, fragmentation, and 200 base pair size selection. RNA-Seq was performed in two lanes of an Illumina HiSeq 2500 with 101 base pair length reads obtained.