DNA-seq of decomposition metagenome: OPEFB bacteria
Hypervariable region of bacterial (V3) and fungal  genes were amplified using  primer specific to each domain. After the PCR, agarose gel electrophoresis were run to test PCR products and the DNA is recover by Sangon agarose recover kit. the primer for PCR was fused with universal primer of Miseq sequencing platform.