GSM2420095: B cell RNA-Seq, rep1; Mus musculus; RNA-Seq
Spleen was dissected from 8-10 week-old EGFP-BLIMP1 mice. To remove red blood cells, the cell suspension was subjected to ammonium chloride lysis buffer (0.15M ammonium chloride, 1.0mM potassium bicarbonate and 0.1mM EDTA), washed with PBS supplemented with 0.1% BSA, and filtered with cell strainer.  The cell suspension was then incubated with rat anti-CD45R/B220 antibody conjugated with PE (BD Biosciences). CD45R/B220 positive cells were purified with FACS. Total RNAs from collected cells were extracted with RNeasy Micro Kit (QIAGEN, 74004) according to the manufacture's instruction. RNA-seq libraries were prepared according to Nakamura et al., 2015, GEO:  GSE63266.
GEO Accession
GSM2420095