LAO-A01 2010-10-04 Metagenomic Sequence Data
DNA paired-end library was constructed with the AMPure XP/Size Select Buffer Protocol as previously described (1), modified to allow for size-selection of fragments using the double solid phase reversible immobilisation procedure described (2). Size selection yielded metagenomic library fragments with a mean of 450bp. All enzymatic steps in the protocol were performed using the Kapa Library Preparation Kit (Kapa Biosystems) with the addition of 1M PCR-grade betaine in the PCR reaction to aid in amplification of high %G C content templates.  1. Kozarewa, I. et al. Amplification-free Illumina sequencing-library preparation facilitates improved mapping and assembly of GC-biased genomes. Nat. Methods 6, 291–295 (2009) 2. Rodrigue, S. et al. Unlocking short read sequencing for metagenomics. PloS One 5, e11840 (2010)