GSM1417153: vegetative somatic cells; Volvox carteri; ncRNA-Seq
Colonies were pelleted and sheared to separate the two different cell types. After size-selection by sieving, the cell preparations were pure. RNA was prepared by using Trizol according to manufacturer's instructions. Library was constructed according to Illumina's small RNA cloning kit v1.5 using the following adapters:  5′adapter 5′-GUUCAGAGUUCUACAGUCCGACGAUC-3′; 3′adapter 5′-Phospho-T-4nt-Barcode-CGTATGCCGTCTTCTGCTTG-(C7amino)-3′. Used barcodes: Sample 1=ACTA
GEO Accession
GSM1417153