16S_V4 amplicons from Cebus capucinus imitator feces
We extracted DNA from 86 fecal samples in a UV sterilized biosafety cabinet using a standard phenol-chloroform extraction with bead beating. The V4 region of the 16S rRNA gene from each of the 86 samples was amplified in triplicate with dual-indexed barcodes and standard primers (515F, 806R). Samples were pooled, tagged with dual indexes, and sequenced on an Illumina MiSeq with V2 chemistry