RNA-Seq of Cow dung
16S rRNA genes were amplified using the universal primer set 515F (5'-GTGCCAGCMGCCGCGG-3') and 907R (5'-CCGTCAATTCMTTTRAGTTT-3'). PCR reaction conditions as followspre-denaturation at 94 for 5 min; A production program consisting of 30 cycles of 94 for 30 s, 52 for 30 s, 72 for 45 s; final elongation at 72 for 10 min. The PCR products were mixed and concentrated, and the mixed product recovered using gel recovery kit, Create a database of recovered PCR products and sequenced on the Illumina Hiseq 2500 Sequencing Platform to analyze the microbial community composition and diversity of bacteria