GSM1632808: 728_7_miRNA_Endo; Bos taurus; miRNA-Seq
Homogenization: A Pro Scientific homogeniser model PRO200 (PRO Scientific Inc., Oxford, CT, USA) was used to homogenise the biopsy in TRIzol® (Life Technologies™, Paisley, UK) with 3 sec pulses 10 times. Clean-up: Total RNA was then cleaned up using the Qiagen RNeasy® MinElute® Cleanup Kit (Qigen Ltd) he Illumina® TruSeq™ Small RNA sample preparation kit was used to prepare 20 cDNA sequencing libraries according to the manufacturer’s instructions.
GEO Accession
GSM1632808