NextSeq 500 sequencing; RNA-seq analysis of frs7;frs12 (frsD) double loss-of function lines at different times of the day ZT8 (2pm) and ZT16 (10pm).
RNA-seq analysis of frs7;frs12 (frsD) double loss-of function lines at different times of the day ZT8 (2pm) and ZT16 (10pm).
Whole seedlings were harvested and frozen in liquid nitrogen.  Wild type Col-0 and frs7-1;frs12-1 mutants were sown in MS-agar plates and stratified for 3 days at 4°C. After stratification, seedlings were grown in SD (8 h light: 16 hours dark) conditions for 10 days. SD (8 h light: 16 hours dark) conditions for 10 days. Seedlings were harvested at ZT8 (2 pm) and ZT16 (10 pm). RNA was isolated using the RNeasy Plant Mini Kit (Qiagen) and DNase I treated (Promega). The library of cDNA template molecules of known strand origin was obtained using the reagents provided in the Illumina TruSeq Stranded mRNA Sample Preparation Kits.
Experimental Factor: genotype
frs7-1;frs12-1
Experimental Factor: time
ZT16