Genome sequencing of Desulfovibrio desulfuricans IC1
Desulfovibrio desulfuricans IC1 was received at the University of Missouri from DSMZ DSM No. 12129 in June 2018. The culture was grown at 30 degrees Celcius in MOYLS4 medium (De Leon KB et al, mBio 8(5): e01696-17, 2017) with the following modifications: potassium phosphate was replaced with 2 mM phosphate buffer (pH 7.2) composed of potassium phosphate (dibasic) and sodium phosphate (monobasic) and in the trace element solution nickel sulfate was replaced with 421 uM nickel chloride. The genomic DNA was extracted using the Wizard Genomic DNA Purification Kit (Promega Corp., Madison, WI). Library was prepared and sequenced at the University of Oklahoma Institute for Environmental Genomics.