Metagenomic-Gut: 27T1
For library preparation using the MiSeq Illumina platform (Illumina, San Diego, CA), the V3-V4 region of the 16S rRNA gene was first amplified using the 341F-806R primers, appended with overhang adapters and barcodes (TruSeq structure adapter). A subsequent amplification step was performed to add multiplexing indices. PCR reactions were performed in triplicate using the Platinum Taq enzyme (Invitrogen, USA).