GSM3938970: PC-3 siELL2-1: siRNA knockdown of ELL2 gene; Homo sapiens; RNA-Seq
Total RNA was isolated with Trizol reagent (15596018, Invitrogen) according to the instruction. cDNA was synthesized with PrimeScript™ RT Master Mix  (RR036A, Takara Bio). Quantitative RT-PCR was conducted to amplify ELL2, STAT1, IRF1 and GAPDH with Real-time qPCR Master Mix (639676, Takara Bio) on ABI Step-One Plus (Applied Biosystems, Foster City, CA). RNA Seq libraries were prepared using Illumina TruSEQ Stranded mRNA kit