GSM2579642: HCT116.Control.WGBS_run1; Homo sapiens; Bisulfite-Seq
Genomic DNA for WGBS was extracted using QIAamp gDNA Purification Kit (Qiagen). For WGBS, 1 μg of genomic DNA from control and 5 µM decitabine treated cells was fragmented by sonication, end-repaired and dA-tailed according to manufacturer’s conditions (New England Biolabs). Methylated NEB Illumina loop adaptor was ligated to DNA (E7370S, New England Biolabs). Ligated library was digested with USER and bisulfite converted using the EZ DNA Methylation Kit (Zymo Research). Libraries were enriched by PCR using EpiMark Hot Start Taq DNA Polymerase (New England Biolabs).
GEO Accession
GSM2579642