GSM4680299: DMSO+Sham 3; Mus musculus; RNA-Seq
Left ventricular muscles of mouse hearts were extracted, flash frozen on dry ice, and RNA was harvested using Trizol reagent and a phenol-chloroform extraction was performed to isolate RNA. Then, total RNA was qualified and quantified using a Nano Drop and Aligent 2100 bioanalyzer(Thermo Fisher Scientific, MA, USA). Oligo(dt)-attached magnetic beads were used to purified mRNA. Then, double strained products were purified, heated denatured and circularized by the splint oligo sequence to get the final library. The single strand circle DNA was formatted as the final library. The final library was amplified with phi29 to make DNA nanoball(DNB). DNBs were loaded into the patterned nanoarray and single end 50 bases reads were generated in BGIseq500 platform.
GEO Accession
GSM4680299