GSM2580021: Midbrain_pAstros; Homo sapiens; RNA-Seq
Cells were plated at 1,000,000 cells per well of a 6-well plate. Within 24-48 hrs, astrocyte cultures were washed with PBS and lysed with RLT buffer (Qiagen, # 74106) or Qiazol reagent (Qiagen). The total RNA was extracted from 30-day differentiated astrocytes with easy mini kit (Qiagen, # 74106) with on-column DNase Idigestion (Qiagen, #79254). RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM2580021