GSM3324706: 9E; Mus musculus; RNA-Seq
Total RNA was isolated from frozen dissected PFC tissue using QIAzol lysis reagent and purified using the miRNAeasy mini kit (Qiagen). For RNA being utilized for RNA-seq, RNA integrity (RIN) was assayed using an Aligent 2100 Bioanalyzer (Aligent, Santa Clara CA). Libraries were prepared using the TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego CA). Briefly, mRNA was polyA selected from the total RNA pool. mRNA was then fragmented and converted to cDNA with reverse transcriptase followed by cDNA size selection and purification with AMPure XP beads (Beckman Coulter, Brea CA). To identify eachsample, strand-specific adapters were ligated to adenylated 3' ends and an additional size selection step was performed. The cDNA library was then amplified using polymerase chain reaction. During this step, barcodes of six base pairs were added to the adaptors. Library quality and concentration was measured by the Bioanalyzer before sequencing.
GEO Accession
GSM3324706