GSM1904280: rat1-1_ORC_ChIP_37C; Saccharomyces cerevisiae; ChIP-Seq
Cells were croslinked with 1% formaldehyde, lysed and sonicated to obtain a DNA shear size of 100-800 bp. Immunoprecipitation was carried out with polyclonal antibodies against ORC or MCM (Gifts from Steve Bell, MIT *). Fragments were sequenced on an Illumina platform and reads were mapped using Bowtie and Bedtools. * Antibodies raised by Steve Bell group: UM185 (polyclonal anti-Mcm2-7) and 1108 (polyclonal anti-Orc1-6) Library was constructed by the sequencing core
GEO Accession
GSM1904280