Crocker Reef Benthic Water Metagenomes
The source of this metagenome is a sample of seawater collected approximately 20 cm above the bottom at a coral rubble area in Crocker Reef. Approximately ten liters of water were collected in duplicate by a peristaltic pump. The action of the pump pushed the water through two inline filters: a 0.45 um prefilter and a 0.22 um Sterivex filter. The sample was divided among two Sterivex filters in order to avoid clogging the filters. After the water was filtered, the Sterivex filters were capped, sealed in plastic bags, and stored in liquid nitrogen until the ship returned to shore and the filters were transported to the Environmental Microbiology Laboratory in St. Petersburg, FL. There the filters were stored in a freezer at -80 degrees Celsius until further processing. Deoxyribonucleic acid (DNA) was extracted from the Sterivex filters using the MO BIO PowerWater Sterivex DNA Isolation Kit following the manufacturer's instructions except for Steps 8 and 9, which were omitted. Steps 8 and 9 call for the heating and subsequent cooling of the filter. Extracted DNA from the two Sterivex filters was pooled in order to obtain one extraction for the sample. The shotgun metagenome (untargeted sequencing of all genomes present in a sample) dataset was produced by construction of a library with a TruSeq Nano DNA Library Prep Kit and sequencing of that library. The library was sequenced on an Illumina MiSeq sequencing system with v3 chemistry by the Center for Genome Research and Biocomputing at Oregon State University.