PacBio sequencing of H. werneckii SMRT5
Genomic DNA for PacBio sequencing was extracted using the Gentra Puregene Yeast/Bact. kit (Qiagen) from two independent 1 ml overnight cultures of H. werneckii grown in YNB containing 1.7 M NaC  (equivalent to 10 % (w/v)) taking care to avoid manipulations (e.g. repeated pipetting) that could shear the genomic DNA. DNA samples were quantified, pooled, and sequenced using five SMRT cells with P4/C2 chemistry at the University of Washington PacBio Sequencing Facility.