16S rRNA sequencing of mouse cecal microbiota
Genomic DNA was  isolated from mouse cecal sample. The DNA concentration was estimated using  Qubit Fluorimeter (V.3.0). The V3-V4 region of 16S rRNA was amplified  using specific V3 Forward primer GCCTACGGGNGGCWGCAG and V4 Reverse primer  ACTACHVGGGTATCTAATCC.The amplified product was checked on 2% agarose gel and  gel purification was done to removed on-specific amplifications. 5ng of  amplified product was used for library preparation using Nextera XT Index   library preparation kit. The library quantification and quality estimation  were done in Agilent 4200TapeStation. The prepared library was sequenced in  Illumina MiSeq with 2*300 cycles chemistry