Illumina HiSeq 4000 paired end sequencing; RNA-seq to investigate effect miRNA 210 knockdown of airway respiratory muscle cells treated with BEAS-2B cell condition media
RNA-seq to investigate effect miRNA 210 knockdown of airway respiratory muscle cells treated with BEAS-2B cell condition media
Airway smooth muscle cells were rinsed with PBS containing Mg and Ca Chloride. Cells were lysed according to manufacturer's protocol (Qiagen RNeasy) Airway smooth muscle cells were cultured in DMEM containing 10% FBS and PSA.  Cells were stimulated with conditioned medium from BEAS-2B cells. Qiagen RNeasy RNA extraction kit was used according to manufacturer's protocol. total RNA from RNeasy extraction kit
Experimental Factor: RNA interference
miR120 knockdown by siRNA