C3xAlt_POOL3
Pooled multiplex fragment libraries of 23 individuals from a segregating F1 population generated from the cross of globe artichoke "C3" genotype and cultivated cardoon "Altilis 41" genotype. DNA samples were fragmented to lengths of ~300 bp by sonication using a Covaris E220 as per the manufacturer’s instructions (Covaris, Inc.). Genomic paired-end libraries were then prepared using standard procedures and NEB reagents for end-repair, A-tailing, and adapter ligation. Fragment size was controlled using Ampure beads purification procedure. Libraries were PCR-enriched using the high-fidelity Phusion polymerase with 5 cycles of amplification. These libraries were prepared with the aim to perform shallow coverage sequencing for genotype imputation purposes.  Individuals are identified with the following barcodes in the header: Art_155:ACTGAT; Art_156:ATGAGC; Art_157:ATTCCT; Art_158:CAAAAG; Art_159:CAACTA; Art_160:CACCGG; Art_161:CACGAT; Art_162:CACTCA; Art_163:CAGGCG; Art_164:CATGGC; Art_165:CATTTT; Art_166:CCAACA; Art_167:CGGAAT; Art_168:CTAGCT; Art_169:CTATAC; Art_170:CTCAGA; Art_171:GCGCTA; Art_172:TAATCG; Art_173:TACAGC; Art_174:TATAAT; Art_175:TCATTC; Art_176:TCCCGA; Art_177:TCGAAG