GSM4437255: uty_15; Mus musculus; RNA-Seq
Nuclei were extracted from frozen samples of left ventricles and labeled with an Alexa fluor 642-coupled antibody  raised against the pericentriolar 1 protein (PCM1) (SCBT, Dallas, TX), i.e. an antigen that accumulates specifically around nuclei from cardiomyocytes.  For each sample, 150,000 cardiomyocyte-specific nuclei were then obtained by cytofluorometry sorting.  Total RNA was obtained using RNeasy micro kits with in-column DNase treatments (Qiagen, Toronto, Canada). RNA-seq library preparation was performed using the SMARTer Stranded Total RNA-Seq Kit v2 - Pico (Takara Bio, Mountain View, CA)
GEO Accession
GSM4437255