GSM3324047: CD34_expt1_rep1; Homo sapiens; RNA-Seq
Cell pellets were treated with QIAzol lysis reagent (Qiagen) and total RNA was isolated using the miRNAeasy kit (Quiagen) combined with on column DNAse (Qiagen) treatment according to manufacaturer's instructions. RNA-seq libraries were prepared from 1 ng of total RNA using the SMART-Seq HT Kit (Takara) combined with Nextera XT kit (Illumina), according to manufacturers' instructions. Briefly, a one-step cDNA synthesis and ds cDNA amplification was conducted with 3' SMART-Seq CDS Primer II A for priming, and SMART-Seq HT oligonucleotide for template switching at the 5' end of the transcript. Then, cDNA was purified with the Agencourt AMPure XP kit, tagmented, and PCR amplified with appropriate index primers.
GEO Accession
GSM3324047