10 microgram of 11026103 per millilitre of culture medium
From log-phase cultures, RNA was isolated with RNAqueous Total RNA Isolation Kit (Invitrogen) and trace DNA was removed with the TURBO DNA-free Kit (Invitrogen). Sequencing libraries were prepared using the ScriptSeq Complete Kit (Bacteria) (Illumina) and sequenced using the MiSeq Reagent Kit v2 (300 cycles) (Illumina).