16S rRNA sequencing of dital colonic content of mus musculus: Cohabitated with Donor 1 ex-germ-free male Control #72
The V3 region of the 16S rRNA was amplified from DNA extracted from distal colonic content. The primers used for the amplification were modified for adaptation to the Illumina platform and included the addition of 6-base pair, unique barcodes to the forward primer, allowing for multiplex amplification. The PCR  products were then sequenced using the Illumina MiSeq platform.