GSM2768984: 293T-124-2; Homo sapiens; miRNA-Seq
RNA was harvested using TRIzol-chloroform. Sequencing libraries were constructed by BGI following the BGI-500 kit, using 1 ug of total RNA subjected to isolation of small RNAs less than 30 nt by gel separation (15% Urea-PAGE). Small RNA fragments were ligated to adenylated 3’ adapters annealed to unique barcodes, followed by the ligation of 5’ adapters and reverse transcription (RT). Samples were pooled to make a single strand DNA circle (ssDNA circle) to yield the final small RNA library, after a second size selection by gel separation. DNA nanoballs (DNBs) were generated with the ssDNA circle by rolling circle replication (RCR) to enlarge fluorescent signals at the sequencing process. The DNBs were loaded into the patterned nanoarrays and single-end reads of 50bp were read on the BGISEQ-500 platform.
GEO Accession
GSM2768984