GSM1904117: WGBSeq EpiSC 6; Mus musculus; Bisulfite-Seq
EpiSCs were grown in feeder-free conditions in fibronectin (Millipore) coated dishes. Culture media contained: N2B27 medium supplemented with bFGF (12 ng/ml) and ActivinA (20 ng/ml). 160 ng of sonicated DNA was used for library preparation using Ovation Ultralow Methyl-Seq Library System (Nugen). This entailed a 3.5 h bisulfite conversion of adapter-ligated DNA using EZ DNA Methylation-Direct (Zymo Research). The DNA was amplified using 7 cycles. Once libraries were prepared they were sequenced using HiSeq2000 with paired-end 100nt read length.
GEO Accession
GSM1904117