GSM2418428: Nonstressed female 2 (NSF2) miRNA; Mus musculus; miRNA-Seq
Small RNA-Seq libraries were generated from enriched small RNA fractions (<200 bp) according to the instructions of the Scriptminer Small RNA-Seq Library Preparation Kit (Epicentre, Madison, WI) with modifications as described in Dias et al. (Nature, 2014). 14-gauge ventral striatum punches were collected from each animal and flash frozen on dry ice. RNA was isolated using homogenization in Qiazol (Qiagen, Hilden, Germany) followed by chloroform layer separation. Bilateral NAc punches from 5 animals (10 NAc punches) were pooled for each library at the point of homogenization. The aqueous RNA layer was further processed via miRNeasy mini kit (Qiagen, Hilden, Germany) to yield separate fractions enriched for small RNA (<200 bp; for small RNA sequencing) and large RNA (>200 bp; for mRNA sequencing).
GEO Accession
GSM2418428