SSU sequencing of deep biosphere: old saline aquifer
Samples were collected by filtration under pressure. Genomic DNA was extracted using the PowerWater DNA Isolation Kit (MO BIO). A bacterial 16S rDNA v3v4 amplicon library for sequencing was generated with primers 341F and 805R.Purified amplicons were sequenced using MiSeq (Illumina) using the p300bp aired-end setting.