RNA-seq of scoliotic zebrafish brains: wildtype sibling with straight spine
3 brains were pooled from 21 dpf fish and RNA extracted with an RNA extraction kit. RNA sample quality was assessed using an Agilent Bioanalyzer. Library prep and subsequent sequencing was performed by The Centre for Applied Genomics at The Hospital for Sick Children (SickKids) using the NEBNext stranded RNA.