GSM3325485: gDNA_acf_rescue; Drosophila melanogaster; MNase-Seq
1.5 mM f.c. CaCl2 and 1.4 x 10-3 U MNase were added to digest the chromatin at 30°C for 15 min. After quenching the reaction with 10 mM f.c. EDTA, samples were treated with 20 RNase mg A for 30 min. Proteins were digested overnight after adding 16 ml 2% SDS and 100 mg Proteinase K and incubating at 37°C. The DNA was purified with the GenElute PCR clean-up kit (Sigma-Aldrich). Libraries were prepared using the MicroPlex Library Preparation kit (Diagenode). PCR amplification was monitored by quantifying amplified libraries.
GEO Accession
GSM3325485