GSM3939097: 2.TP53R273H; Homo sapiens; miRNA-Seq
RNa and miRNA were extracted in vitro or ex vivo using qiagen kits per manufacturer's instruction mRNA: Illumina Compatible low input RNA libraries were prepared using the Smart-Seq V4 Ultra Low Input RNA (Clontech) and KAPA HyperPlus Library Preparation kits. The libraries were then sequenced on the Illumina HiSeq4000 sequencer using the 76bp paired end format. miRNA: Illumina compatible miRNA libraries were prepared using the QIAseq miRNA Library Kit (Qiagen). The libraries were then sequenced on the Illumina NextSeq500 Sequencer using the 76nt SR High-Output flow cell.