16S amplicons from soils samples: sugarcane monoculture for 100 years: Sample SC1007
In May 2011, at the end of the rainy season, agricultural and forest soils samples were collected from various sites across the central-northern region of the Argentinean Southern Andean Yungas, covering areas dedicated to sugarcane and soybean crops. Agricultural sites were formerly pedemontane forest lands of the Yungas then deforested in different moments of a 100-year period. Primary forest remains adjacent to agricultural plots. Bulk soil samples were collected from all these sites to conform three land-use categories: long-term agriculture (30-100 years), short-term agriculture (2-5 years) and pristine forest (undisturbed). Total DNA was extracted from 0.25 g of each soil sample using the MoBio Power Soil DNA isolation kit according to manufacturer’s instructions. The concentration and quality (A260/A280) of the extracted DNA were determined using a GeneQuant RNA/DNA calculator spectrophotometer (Pharmacia Biotech). Amplicons for barcoded pyrosequencing were generated by PCR using the primer set 515F (GTGCCAGCMGCCGCGGTAA) and 806R (GGACTACVSGGGTATCTAAT), targeting the V4 region of the bacterial 16S rRNA gene. Unidirectional sequencing of the amplicon libraries was performed from the forward primer at Macrogen Inc. (Seoul, South Korea) using the 454-GS-FLX Titanium system. Sequence processing and filtering and data analyses were performed with mothur 1.31.2. 