Tissue-specific transcriptomes from Avicennia marina (Flowers, Leaves, Roots, Seeds, Stems, and Pooled)
Total RNA was isolated from root, stem, leaf, flower, and seed tissues in addition to the pooled sample using TRIzol reagent (Invitrogen, USA) following the manufacturers instructions. The A. marina RNAseq libraries were prepared using TruSeq RNA sample prep kit (Illumina, Inc.) following the manufacturers instructions. Briefly, TruSeq RNA sample prep kit converts the poly-A containing mRNA in total RNA into a cDNA library using poly-T oligo-attached magnetic bead selection. Following mRNA purification, the RNA is chemically fragmented before reverse transcription and cDNA generation. The fragmentation step results in an RNAseq library that includes inserts that range in size from approximately 100-400 bp. Library quality control and quantification were performed with a Bioanalyzer Chip DNA 1000 series II (Agilent) and sequenced directly using the high-throughput Illumina HiSeq sequencing system (Illumina, Inc.).