GSM1416932: BD60; Macaca mulatta; Bisulfite-Seq
DNA and RNA extraction was performed using AllPrep DNA/RNA mini kit (Qiagen). Genome-wide methylation data was generated for each monkey using reduced representation bisulfite sequencing (RRBS). Briefly, genomic DNA (1ug) was digested with MspI and the fragments were end-repaired and ligated to Illumina Truseq adapters (methylated) following the manufacturer’s protocol (Illumina). Adapter-ligated DNA fragments, ranging from 200 to 400 base pairs (bp), were purified by gel electrophoresis (2.5% NuSieve GEG agargose) and then treated with sodium bisulfite (ZymoResearch EZ DNA methylation gold kit), which converts unmethylated cytosines to uracil and leaves methylated cytosines unchanged. Converted libraries of DNA fragments were then amplified using TruSeq adapter primers (Illumina) and amplicons were purified (MiniElute PCR purification kit; Qiagen 28004) and sequenced on a Next-Generation sequencer (Illumina HiSeq2000).
GEO Accession
GSM1416932