GSM1417149: Spontaneously hypertensive rat (SHR); Rattus norvegicus; RNA-Seq
Trizol extraction of total RNA was performed according to the manufacturer's instructions. The rRNA depleted total RNA was reverse transcribed, fragmented, and 3’ end repaired, after adding a “dA” base to the 3’ end, the Illumina adapter was ligated to the double strand cDNA. The adpter ligated library was then amplified using Illumina’s proprietary amplification primers. Final product was purified by PAGE gel. ~200-300 bp fragments were selected at the gel size fractionation step. The final size of the library was confirmed by Agilent 2100 Bioanalyzer using DNA 1000 Kit.
GEO Accession
GSM1417149