Illumina HiSeq 2000 paired end sequencing; Changes in the coding/non-coding transcriptome and DNA methylome that define the Schwann cell repair phenotype after nerve injury
Changes in the coding/non-coding transcriptome and DNA methylome that define the Schwann cell repair phenotype after nerve injury
Sciatic nerves of adult mice were cut or crushed at the sciatic notch. DNA was isolated from samples using phenol chloroform method.  Libraries were prepared without pooling to generate methylation profiles on the HiSeq 2000 platform to generate 100bp pair end reads as described in (Johnson et al 2012: DOI: 10.1002/0471142727.mb2123s99)
Experimental Factor: methylation profiling by high throughput sequencing
protocol
Experimental Factor: cut nerve
injury
Experimental Factor: 7
time