RNA-Seq of homo sapiens: adult female adipocytes differentiated in vitro from adipose mesenchymal stem cells on patterned islands under fiber networks
RNA was extracted from ~ 175,000 cells per sample using a RNeasy Lipid Tissue Mini Kit (Qiagen, 74804). Extracted RNA was run on a BioAnalyzer RNA Pico chip (Agilent, 5067-1513) and all samples sequenced had RNA Integrity Numbers above 8. Isolation of mRNA and library preparation were performed using a KAPA mRNA HyperPrep kit (Roche, 08098115702) and libraries were sequenced on an Illumina NextSeq 500 sequencer.