Solid-state acetic acid fermentation Days-7 genome V4 region of 16S rRNA sequencing
PCR was carried out using genomic DNA as template targeting the V4 region of 16S/18S rRNA genes, PCR products were purified with GeneJET Gel Extraction Kit (Thermo Scientific). Sequencing libraries were generated using NEB Next® UltraTM DNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations and index codes were added. The library quality was assessed on the Qubit@ 2.0 Fluorometer (Thermo Scientific) and Agilent Bioanalyzer 2100 system. Finally, the library was sequenced on an Illumina MiSeq platform using paired-end sequencing.