GSM2579252: PET111GFP MZ 6DO rep 1; Arabidopsis thaliana; RNA-Seq
Roots were cut and protoplasted. GFP negative cells were collected using a MoFlo cell sorter in RLT buffer and beta mercaptoethanol. RNA was extracted from sorted cells using the Qiagen RNeasy Micro Kit cDNA synthesis and amplification was performed using the SMARTer Low Input RNA Kit for sequencing. Fragmentation was done using Covaris ultrasonicator. Libraries were prepared using the Low Input Library Prep Kit.
GEO Accession
GSM2579252