DYNA_08Novembre_F93Disque
Nucleic acids were extracted from two 3-m pore size filters and two 0.22-m Sterivex units. A first PCR step performed to amplify the targeted DNA region (bacterial and archaeal 16S rRNA gene and eucaryotic 18S rRNA gene). Illumina MiSeq adaptors and barcodes were added during the second PCR step (dual indexed with multiple identifiers).