GSM2760046: Person 3 hypothyroid; Homo sapiens; RNA-Seq
Whole blood samples were collected in PAXgene tubes(PreAnalytiX, Hombrechtikon, Switzerland,catalog number 762165 ). RNA was isolated using PAXgene blood miRNA kits (PreAnalytiX, Hombrechtikon, Switzerland, catalog number 763134 ). For RNAseq analysis ribosomal RNA and globin mRNAs were first removed from an aliquot of the RNA samples using the Globin-Zero Gold rRNA Removal Kit (Illumina, San Diego, USA). Kapa Stranded RNA library was prepared (Kapa Stranded RNA kit, Kapabiosystems), followed by sequencing on a HiSeq2500 system, for single end reads, 50 bp in length (Illumina, USA).