GSM2579276: MEF_RNA-seq_rep2; Mus musculus; RNA-Seq
For RNA-seq, the cells were lysed using RLT buffer supplemented with β-mercaptoethanol and processed using the RNeasy mini kit (Cat. 74104).For ChIP-seq, the cells were cross-linked with 37% formaldehyde in serum-free medium at room temperature for 10 min in room temperature (RT). Glycine was added to stop the cross-linking at final concentration of 0.125 M and the cells were rinsed in PBS. Then the cells were re-suspended in lysis buffer containing protease inhibitors and chromatin was sonicated to 100-400 bp with Covaris M220 system. RNA-seq: KAPA Stranded mRNA-Seq Kit (KK8420) ChIP-seq: KAPA HyperPlus Library Preparation kit (KAPA KK8510)
GEO Accession
GSM2579276