16S rDNA-Seq, microbial diversity in Chinese paddy soil
Three DNA extractions per sample were conducted using a FastDNA Spin Kit for Soil (MP Bio, Santa Ana, CA, USA) following the manufacturers procedures. The primer of 16S rDNA sequence is 338F (ACTCCTACGGGAGGCAGCA) and 806R (GGACTACHVGGGTWTCTAAT). The samples were run on Illumina MiSeq following the manufacturers instructions.