DNA-Seq of Sphenodon punctatus: adult male blood: 2x100 MP 5000 insert
Total genomic DNA was extracted using proteinase K digestion and Phenol-Chloroform extraction from blood. Sequencing was undertaken using the Illumina HiSeq 2500 sequencing platform (Illumina, San Diego, CA, USA). Sequencing libraries consisted of mate pair (MP) libraries with estimated insert size of 5 kbp. The paired end libraries were prepared using the Illumina TruSeq PCR-Free DNA library kit as per manufacturers instructions. These libraries were normalised and pooled across 3 lanes of Illumina HiSeq 2000 or 2500 using 2 x 100 bp paired end sequencing at New Zealand Genomics Ltd., Dunedin.