GSM3558041: ctrl_f_2; Homo sapiens; miRNA-Seq
Peripheral blood mononuclear cells (PBMC) were isolated using Ficoll-Hypaque density gradient method within 3 h of sampling. RNA was isolated using miRNeasy Mini Kit. Small RNA libraries were constructed using TruSeq® Small RNA Library Prep Kit, following manufacturer instructions. Individual libraries were constructed using 1µg of total RNA isolated from each sample. Samples were individually barcoded and sequenced in multiplexed pools each containing 4 samples using the 36 bp fragment protocol on Illumina MiSeq platform
GEO Accession
GSM3558041