GSM1633579: wt4ko; Mus musculus; MNase-Seq
Tissue samples were washed in cold PBS and re-suspended in RSB buffer (10 mM Tris-HCl, 10 mM NaCl, 3 mM MgCl2, 0.2% NP-40, protease inhibitors). Nuclei pellets were digested with Micrococcal Nuclease in the presence of 1 mM CaCl2. Reactions were terminated by adding 10 mM EDTA. The chromatin preparations were then purified using a QIAGEN PCR Purification Kit. Mononucleosome was used for downstream library preparation. Libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM1633579