GSM3175517: TQ325-IgG; Homo sapiens; ChIP-Seq
HCASMC were crosslinked in the dish, 2 million cells, and harvested. ChIP was performed using the SMAD3 antibody Abcam, ab28379, and library was prepared using standard procedures. Briefly, DNA was prepared for end repair (Lucigen Endi-it, ER0720) and “A” tailing (NEB Klenow, M0212S), adaptor ligation (Promega, M180A), and library amplification (NEB, M0531S). ChIP-seq libraries were sequenced on HiSeq X10 for 150-bp paired-end sequencing.
GEO Accession
GSM3175517