GSM2839029: ChIPseq_H3K4me3Q5ser_Day_0_3; Rattus norvegicus; ChIP-Seq
RN46A-B14 cell pellets (10 x 106/n) and DRN tissues were crosslinked and quenched. Samples were washed thoroughly before being subjected to lysis and sonication. Samples were then incubated with specific antibodies (7.5 μg/sample) bound to M-280 Dynabeads on a rotator at 4°C overnight. The following day, immunoprecipitates were washed, eluted and reverse crosslinked. Following RNA and protein digestion, DNA fragments were purified using a Qiagen PCR purification kit. RNA and ChIP libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM2839029