DNA-Seq of Sphenodon punctatus: adult male blood: 2x100 MP 2500 insert
Total genomic DNA was extracted using proteinase K digestion and Phenol-Chloroform extraction from blood. Sequencing was undertaken using the Illumina HiSeq 2000 sequencing platform (Illumina, San Diego, CA, USA). The sequencing library consisted of a mate paired (MP) library with estimated insert sizes of 2.5 kbp. The mate pair library was prepared using the Illumina TruSeq DNA library kit as per manufacturers instructions. This library was run on 1 lane of Illumina HiSeq 2000 using 2 x 100 bp paired end sequencing at New Zealand Genomics Ltd., Dunedin.