pH8.2a
Sequencing libraries were prepared using the Genomics in house protocol for mRNA-seq. The polyA fraction (mRNA) was purified from 500 ng of total RNA following by fragmentation and the generation of double-stranded cDNA. Then, end repair, A base addition, adapter ligation and PCR amplification steps were performed. 150 bp PE reads were sequenced on an Illumina HiSeq High Output.