GSM3324362: Ging 3: TCRgd cells from the gingiva; Mus musculus; RNA-Seq
RNA was isolated from cells using the Norgen Biotek Corp Single Cell RNA Purification Kit (Cat. 51800) Total RNA was sent to LC Sciences for sequencing service. The quantity and purity of total RNAs were assessed using a NanoDrop ND-1000 spectrophotometer (NanoDrop Inc., Wilmington, DE, USA). The integrity of total RNAs was analyzed using an Agilent 2100 Bioanalyzer system and RNA Pico Chip (Agilent Tech, Santa Clara, CA, USA). The cDNAs were constructed from total RNA (0.7 ng of each sample) using the SMART-Seq v4 Ultra Low Input RNA Kit from Clontech/Takara according to the manufacturer's protocol. The amplified and purified cDNAs were validated using the Agilent 2100 Bioanalyzer (High Sensitivity DNA Kit).  The libraries for sequencing were prepared using Nextera XT DNA Sample Preparation kit from Illumina and following the manufacturer's protocol (input cDNA: 1ng).   Before sequencing, libraries were qualified on the Bioanalyzer High Sensitivity DNA Chip. Libraries were subsequently pooled and sequenced on the HiSeq X Ten platform in the 150 bp PE configuration.
GEO Accession
GSM3324362