GSM4629665: cerebral organoids 90days old CTRL rep1; Homo sapiens; RNA-Seq
Organoid/neurons were dissociated for scRNAseq using Worthington papainesystem according to manufacturers instruction.RNA samples for total RNAseq were extracted with Trizol and ribodepleted using RNase H-based protocol. Total RNAseq libraries were generated according to Illumina sequencing protocol, using  ribo-depleted RNA samples as a starting material. We carried out single-cell RNAseq using Nadia instrument Dolomite bio (neurons) or Drop-seq instruments (organoids), libraries were prepared according to published protocol (Alles et al., 2017) For scRNAseq we have applied Nextseq, for totalRNAseq we have applied Multiplexed 2x76+8In, Truseq
GEO Accession
GSM4629665