GSM2152749: ac2; Xenopus tropicalis; RNA-Seq
Total RNA was extracted using the acid guanidinium thiocyanate method of Chomczynski and Sacchi (1987: Analytical Biochemistry 162:156-159). Final RNA pellets were resuspended in DEPC-treated water and then precipitated overnight at 4oC in 2.5M LiCl to remove contaminating genomic DNA). Agilent Bioanalyzer analysis revealed all RIN scores to be above 9. The Illumina TruSeq kit was used to isolate polyA+ RNA and construct non-stranded libraries. All cleanup steps used Ampure XP beads. Library quality was assessed by Agilent Bioanalyzer and KAPA qPCR, they were multiplex sequenced and bar-codes on Illumina adaptors were used for de-multiplexing.
GEO Accession
GSM2152749