WGS of E. coli O26:  Composite soil from not recently grazed paddock on 1-Mar-2016
High quality genomic DNA was extracted from each selected isolate using the QIAamp DNA Mini Kit (QIAGEN, Hilden, Germany) following the manufacturer guidelines. DNA paired-end libraries were prepared from a volume of 80 to 100 L of each eluted DNA (155 ng/L) using the Illumina Nextera XT DNA Library Preparation Kit, version 2 (Illumina, Inc.). The resulting libraries with 683 bp inset sizes were sequenced on the Illumina MiSeq Next Generation Sequencing platform (Illumina, Inc.) using version 3 chemistry