GSM3324234: Human placenta 1 Capture-seq; Homo sapiens; RNA-Seq
Total RNA was purified from term placentas using a combination of TRIzol and Qiagen columns. RNA-seq libraries were prepared using the SureSelect Strand-Specific RNA Library Prep for Illumina Multiplexed Sequencing kit (Agilent). For capture-sequencing, pre-amplified RNA-seq libraries were enriched for THE1B containing transcripts using a custom panel of 120 nt THE1B RNA oligos and the SureSelectXT RNA Target Enrichment kit (Agilent). Libraries were sequenced as 75 bp paired-end reads on an Illumina HiSeq machine.
GEO Accession
GSM3324234