Illumina HiSeq 2500 paired end sequencing; Isoform-specific depletion of U2AF1
Isoform-specific depletion of U2AF1
Cell lines were grown under standard conditions in DMEM supplemented with 10% (v/v) bovine calf serum (Gibco BRL) Transfections of small interfering RNAs (siRNAs) were carried out in 6- or 12- well plates using jetPRIME (Polyplus) according to manufacturer's recommendations. The cells received treatment with siRNA twice - 24h post seeding, and 48h post seeding when splitting into a new plate. PolyA library selection was performed using NEBNext poly(A) mRNA magnetic isolation module (E7490L) according to the manufacturer's specifications.
Experimental Factor: library selection
PolyA+
Experimental Factor: transfection
U2AF1 ab siRNA