GSM1531828: rdr3_1_smRNA; Colletotrichum higginsianum IMI 349063; RNA-Seq
Total RNA was isolated from 4 day old mycelia using Trizol reagent. Small RNAs were isolated from 40 µg of total RNA, and size fractionated following the protocol described in Gilbert et al., (2014, Preparation of Multiplexed Small RNA Libraries from Plants. Bio-protocol 4(20): e1275) Libraries were prepared following the detailed protocol described in Gilbert et al., (2014, Preparation of Multiplexed Small RNA Libraries from Plants. Bio-protocol 4(20): e1275); Modifications: 5 µl of size fractionated small RNAs were used as input material; 3' ligation was performed for 2 hr.
GEO Accession
GSM1531828