DNA seq of irradiated Phomopsis sp.
Sequencing on the Genome Analyzer II consists of three stages:. incorporation of fluorescent reversible terminators by DNA polymerase(Figure 2.2n);. imaging of the fluorescently labeled clusters (Figure 2.2o); and cleavage andunblocking of the incorporated nucleotide before the next addition cycle(Figure 2.2p).The single-base extension cycle is repeated for 50 cycles (Figure 2.2q) to generatethe DNA sequence of the >6  107 clusters simultaneously.