GSM2837423: Yng_28_H3; Homo sapiens; ChIP-Seq
Micro-ChIPseq was performed using the True MicroChIP (Diagenode, #C01010130) kit and 0.5 ug H3 (Abcam ab10799, lot #GR275925-1) per immunoprecipitation ChIP-seq libraries were prepared using the V1 MicroPlex Library Preparation kit (Diagenode, #C05010011). For the PCR amplification, a total of 16 amplification cycles was used. Libraries were purified using a 1:1 Ampure bead cleanup and eluted in 16 uL of Tris-HCl ph 8.0. Libraries were multiplexed and sequenced using a NextSeq 500 with 75bp single end sequencing.
GEO Accession
GSM2837423