NextSeq 500 paired end sequencing; RNA of Small Cell Lung Cancer Circulating Tumour Cells Derived Explants
RNA of Small Cell Lung Cancer Circulating Tumour Cells Derived Explants
Circulating tumour cells were collected from patient blood using RosetteSep (Stemcell Technologies) and injected into the flank of non-obese diabetic severe combined immunodeficient interleukin-2 receptor γ–deficient mice.  Tumours were excised from terminally sacrificed mice, and tumour fragments were immediately transferred to 10 volumes of RNAlater (Qiagen) and stored overnight at 4°C.  Tumour fragments were then stored at -80°C until further processing. Three independent tumours from each model were homogenized in RLT buffer (Qiagen) using the TissueLyser LT (Qiagen) and FastPrep Lysing Matrix A tubes (MP Biomedicals).  RNA was extracted with RNeasy mini kits (Qiagen) and quantified using Qubit (Thermo Fisher). RNA-seq data were aligned to Human GRCh38 and Mouse GRCm38 assembly using Mapsplice (version 2.1.6). Mouse reads were subsequently filtered from the data.
Experimental Factor: response to treatment
progressive disease
Experimental Factor: clinical information
baseline