RNA-Seq of human: adult female cervical
After LCM, the mRNAs were enriched using Oligo(dT) beads, fragmented, and reverse transcribed into cDNA with random primers. The second-strand cDNAs were synthesized by DNA polymerase I. The cDNAs were purified with the QiaQuick PCR extraction kit, end-repaired, poly(A)-added, and ligated to Illumina sequencing adapters. The ligation products were size-selected by agarose gel electrophoresis, PCR-amplified, and sequenced using an Illumina HiSeq 2500 by Gene Denovo Biotechnology Co. (Guangzhou, China).