GSM4002848: In vitro plant material, Ultrasonicated 24 hours; Solanum tuberosum; RNA-Seq
Total RNA was purified from the 10 potato samples as three biological replicates each that were pooled using the Direct-zol™ kit (Zymo Research, Irvine, CA, USA) with TRIzol reagent based on the manufacturer's protocol. Using the Illumina TruSeq Stranded mRNA kit, mRNA was first randomly fragmented by adding fragmentation buffer, then cDNA was synthesized with an mRNA template and a random hexamer primer, after which a custom second-strand synthesis buffer, dNTPs, RNase H and DNA polymerase I were added to initiate second-strand synthesis. The second step involved terminal repair, A ligation and sequencing adaptor ligation, after which the double-stranded cDNA library was completed through size selection and PCR enrichment.
GEO Accession
GSM4002848