Illumina MiSeq sequencing; qiita_ptid_6045:12189.DIVSTATS116S
An incubation study using thermally altered pine needles in a pine plantation soil; soils were harvested at 43 days after exposure.
Target amplicon: 16S. Image analysis was performed in real time by the MiSeq Control Software (MCS) v2.6.2.1and Real Time Analysis (RTA) v1.18.54, running on the instrument computer. RTAperforms real-time base calling on the MiSeq instrument computer. Then the Illuminabcl2fastq 2.20.0.422 pipeline was used to generate the sequence data. The data generatedhere meet the AGRF quality standards.
barcode
CCTAAGACCCTAGAGT
center_name
AGRF
flowcell_id
V1V3
lane
1
orig_name
sample1
pcr_primers
(FWD:AGAGTTTGATCMTGGCTCAG; REV:GWATTACCGCGGCKGCTG)
primer
AGAGTTTGATCMTGGCTCAG
primer_forward
AGAGTTTGATCMTGGCTCAG
primer_reverse
GWATTACCGCGGCKGCTG
run_prefix
DIVSTATS1216S
sequencing_meth
Sequencing by synthesis
target_gene
16S rRNA
target_subfragment
V13