GSM3323415: gDNA_control_Rep1; Drosophila melanogaster; ATAC-seq
Fragmentation and amplification of the ATAC-seq library was performed according to the standard protocol (Buenrostro et al., Current Protocols in Molecular Biology, 2015). 12 ng of genomic DNA was combined with 2.5 μl of Nextera Tn5 transposase in Nextera Tagment DNA Buffer (Illumina) to the final volume of 50 μl. After 12 PCR amplification cycles, the library was purified with Agencourt AMPure XP beads (Beckman Coulter: A63881), using double size selection (left ratio: 2.0x, right ratio: 0.5x).
GEO Accession
GSM3323415