Illumina MiSeq sequencing; qiita_ptid_6079:12189.DIVSTATS6ITS
An incubation study using thermally altered pine needles in a pine plantation soil; soils were harvested at 43 days after exposure.
Target aplicon: ITS. Image analysis was performed in real time by the MiSeq Control Software (MCS) v2.6.2.1and Real Time Analysis (RTA) v1.18.54, running on the instrument computer. RTAperforms real-time base calling on the MiSeq instrument computer. Then the Illuminabcl2fastq 2.20.0.422 pipeline was used to generate the sequence data. The data generatedhere meet the AGRF quality standards.
barcode
CGAGGCTGTCGACTAG
center_name
AGRF
flowcell_id
V1-V3
lane
1
orig_name
DIVSTATS6-ITS
pcr_primers
FWD: CTTGGTCATTTAGAGGAAGTAA; Rev: GCTGCGTTCTTCATCGATGC
primer
CTTGGTCATTTAGAGGAAGTAA
primer_forward
CTTGGTCATTTAGAGGAAGTAA
primer_reverse
GCTGCGTTCTTCATCGATGC
run_prefix
DIVSTATS6ITS
sequencing_meth
sequencing by synthesis
target_gene
ITS
target_subfragment
ITS1/2