GSM3689777: rat; Rattus norvegicus; RNA-Seq
CD45+ innate immune cells (T/B cell negative CD45+ cells) were sorted using a BD FACS AriaII. Live single cells were loaded into the 10x genomics machine. Full length cDNA libraries were prepared by incubation of Gel Bead-In-EMulsions (GEMs) in a thermocycler machine with Chromium v2. GEMs containing cDNAs were broken and all single cell cDNA libraries were pooled together, cleaned using DynaBeads MyOne™ Silane beads (Fisher PN 37002D), and pre-amplified by PCR to generate sufficient mass for sequencing library construction.
GEO Accession
GSM3689777