GSM4666066: MUC2953; Homo sapiens; RNA-Seq
RNA from skin biopsies was isolated using the QIAzol Lysis Reagent (Qiagen) and miRNeasy Mini Kit (Qiagen) according to manufacturer's protocol RNASeq libraries were generated using the TruSeq Stranded Total RNA Kit (Illumina) with Ribo-Zero Depletion (rRNA Removal Mix) according to manufacturer's high sample protocol. 200 ng of RNA was used as input. DNA was PCR amplified with Illumina primers for 13 cycles. Libraries were sequenced on the Illumina HiSeq 4000 as paired-end with a read length of 2x 150 bp and an average output of 40 Mio reads per sample and end following the manufacturer's protocols.
GEO Accession
GSM4666066