GSM2151148: CysNO treatment: Control_2; Arabidopsis thaliana; RNA-Seq
RNA was extracted from triplicate leaf samples of treated and control plants using RNeasy® Plant Mini Kit (Qiagen) according to standard protocol provided by the manufacturer. RNA was purified using miRNeasy Kit (Qiagen), following the manufacturer’s instructions. Quality of RNA was analyzed by Agilent 2100 Bioanalyzer QC and samples with RIN(RNA Integrity Number) 7 ≥ and 28s/18s ratio 1.0 ≥ were used for c DNA synthesis. Library was prepared using TruSeq RNA library prep kit (Illumina) for 100bp paired-end sequencing.  Briefly, poly A tailed transcript RNA was isolated using oligo(dT) selection from 2µg total RNA and ramdomly fragmented by Mg 2+  ion treatment. The fragmented mRNA was subjected to cDNA synthesis through random hexamer priming. End-repair, A-tailing and Adapter ligation was conducted for Blunt-end producing and Adapter genernation. After that c DNA was amplified by PCRcDNA libraries were quantified by KAPA library quantification kit (Illumina). Samples were sequenced using Hiseq-2500 sequencer (Illumina)
GEO Accession
GSM2151148