RNAseq in Homo sapiens: adult CN3 MT
Total RNA was isolated using the miRNA Tissue kit on an automated Maxwell RSC extractor (Promega), following the manufacturer's instructions. RNA integrity was assessed on a TapeStation (Agilent). Subsequently, 2 g of RNA for each sample were used to generate single-end 75-bp sequencing libraries with the TruSeq Stranded mRNA Library Prep Kit (Illumina), according to the manufacturer's protocol. Sequencing was performed on a NextSeq500 (Illumina)