GSM2420389: hyp2-1_rep3; Saccharomyces cerevisiae; OTHER
Cells were harvested and pellets were frozen in liquid nitrogen and kept at -80°C until further processing. Total RNA was extracted from yeast cells using phenol:chloroform extraction. 5PSeq methods was performed as previously described (PMID: 26820793) with minor modifications. 1µg of total RNA was used as input. After single-stranded RNA ligation, partial rRNA depletion was performed using mammalian RiboCop rRNA depletion kit (Lexogen). 5PSeq libraries were PRC amplified (18 cycles), pooled and size selected (300-500nt). Samples were sequenced using a NextSeq500 producing single-end 75 reads.
GEO Accession
GSM2420389