Miseq analysis of soil samples
Genomic DNA were amplified, normalized using the SequalPrep Normalization Kit (Life Technologies, Carlsbad, CA, USA) and pooled prior to sequencing on the MiSeq platform. The amplicon pools were prepared for sequencing with AMPure XT beads (Beckman Coulter Genomics, Danvers, MA, USA) and the size and quantity of the amplicon library were assessed on the LabChip GX (Perkin Elmer, Waltham, MA, USA) and with the Library Quantification Kit for Illumina (Kapa Biosciences, Woburn, MA, USA), respectively.ć