Avocado_M223-01-T07_good
Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. The RNA-seq was performed on three biological replicates for each sample used. Fragmentation was proceeded through divalent captions under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5X). First-strand cDNA was synthesized using a series of random hexamer primer and reverse transcriptase, and second-strand cDNA was subsequently produced using DNA Polymerase I and RNase H. The cDNA library was constructed through ligating cDNA fragments to sequencing adapters and carried out PCR amplification. The library preparations were sequenced on an Illumina HiSeq 2000 platform (Nanxin Bioinformatics Technology Co., Ltd., Guangzhou, China).