GSM4628317: ML3288 (OAY470 LEU2::-gal1/10-GapR) in glycerol and induced with galactose, replicate 2; Saccharomyces cerevisiae; ChIP-Seq
ChIP was performed as previously reported (Guo et al, 2018). ChIP antibody is Mouse monoclonal anti-FLAG from Sigma (Cat #: F1804). Libraries were constructed by end repairing DNA with T4 DNA polymerase (NEB), T4 PNK (NEB), and Klenow large fragment (NEB). 3' overhangs were added with Klenow (3'→5' exo-) (NEB). Y-shaped Illumina adapters were ligated onto the DNA, and libraries were amplified with Kapa HiFi Polymerase (Kapa Biosystems). Libraries were size selected to be 200-500 bp.
GEO Accession
GSM4628317