UCE target enrichment of Pserratus_BDT148: tail tissue
DNA extracts containing between 0.5 _g and 19 _g (average: 6 _g) of DNA at a concentration between 16 ng/_L and 270 ng/_L (average: 100 ng/_L) were sent to RAPiD Genomics (Gainesville, Florida) for library preparation, sequence capture, and sequencing. Samples were barcoded using standard Illumina TruSeq adapters with a unique 8 bp index for each individual. Our probe set consisted of 2,064 probes targeting 1,745 UCE loci, a subset of the full tetrapods UCE probe set of 5,472 probes (see Faircloth et al. 2012 and http://ultraconserved.org for probe development).