metagenome of subglacial sediment
0.3 g sediment was sampled for DNA extraction using FastDNA SPIN Kit for Soil (MP Biomedicals, USA). Introduction of this kit was modified for better extraction effect. 970 ul Sodium Phosphate Buffer, 130 ul MT Buffer was added to sediment for DNA extraction. In addition, 40 HZ for 30 s breaking step and followed 30 s interval step were performed 6 times for more effective cell breaking. Other steps was processed following the instruction. Extracted DNA was measured concentration using Quibit Fluorometer (Thermo fisher scientific, USA). Completeness of DNA was evaluated by agarose gel electrophoresis. Qualified DNA was then used for constructing library and later sequenced in Illumina Hiseq 2000 Platform.