GSM2418883: delset2_CHD1-MYC; Saccharomyces cerevisiae; ChIP-Seq
Cross-linked chromatins were sheared using a sonicator, and immunoprecipitated using the following beads or anti-bodies: anti-Myc conjugated agarose bead (Sigma Aldrich, cat.# E6654) to pull-down Chd1 in the Chd1-13XMyc strains (WT and ten elongation factor mutants), and H3K4me3 antibody (EMD Millipore, cat.# 07-473) and H3K36me3 antibody (Abcam, cat.# ab9050) to pull-down H3K4me3/H3K36me3, respectively, in WT and CHD1 deletion mutant. The NEBNext ChIP-Seq Library Preparation Master Mix Set for Illumina (New England BioLabs, cat.# E6240L) was used to sequence the samples using Illumina HiSeq 2500.
GEO Accession
GSM2418883