GSM4192599: Lip_Control_14; Homo sapiens; RNA-Seq
For library preparation and sequencing, total RNA was extracted from n=15 lipomas and the matched normal SAT using RNeasy® Lipid Tissue Kit (Qiagen). The quality and quantity of the purified RNA was determined by measuring the absorbance at 260/280 nm and 260/230 nm using a NanoDrop spectrophotometer and by gel electrophoresis to visualize the 28S and 18S rRNA bands as indicators of total RNA integrity Indexed cDNA libraries were constructed with an average insert size of 300 bp using the TruSeq RNA sample preparation kits v2 (Illumina, San Diego, CA). Libraries were sequenced on an Illumina HiSeq platform as 101 bp paired-end reads to an average of 46.9 million reads per library.
GEO Accession
GSM4192599