Illumina HiSeq 1500 paired end sequencing; Whole Transcriptome sequencing TSCC (tongue squamous cell carcinoma)
Whole Transcriptome sequencing TSCC (tongue squamous cell carcinoma)
Stored in RNA later in liqN2 Total RNA was extracted from tongue primary paired normal-tumor samples and cell lines using RNeasy RNA isolation kit (Qiagen) and Trizol reagent (Invitrogen) based methods and later resolved on 1.2% Agarose gel to confirm the RNA integrity. Transcriptome libraries for sequencing were constructed according to the TruSeq RNA library protocol (Illumina). Briefly, mRNA was purified from 4 μg of intact total RNA using oligodT beads and library preparation was done as per manufacturer's instructions (TruSeq RNA Sample Preparation Kit, Illumina).
Experimental Factor: normal
disease
Experimental Factor: AD0731_29N_tra
individual