GSM2340127: Mutant rep 1; Sus scrofa; RNA-Seq
Embryos were harvested from the uterus and stored in liquid nitrogen for RNA extraction. The extra-embryonic tissues of each embryo were collected for DNA extraction and genotyping of the VRTN QTNs. Total RNA was isolated from three wild-type (qq) and three mutant (QQ) pig embryos using Trizol reagent, which was then used to construct cDNA libraries using the TruSeq RNA Sample Preparation kit (Illumina). RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM2340127