GWD2 RNA reads
RNA was extracted from the 0.2 mm filters using the Invitrogen TRIzol® reagent, followed by genomic DNA removal and cleaning using the Qiagen RNase-Free DNase Set kit and the Qiagen Mini RNeasyTM kit. An Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA) was used to assess the integrity of the RNA samples. The Applied Biosystems SOLiD(TM) Total RNA-Seq kit was used to generate the cDNA template library. The SOLiD(TM) EZ Bead system (Life Technologies, Grand Island, NY) was used to perform emulsion clonal bead amplification to generate bead templates for SOLiD(TM) platform sequencing. Samples were sequenced at Pacific Northwest National Laboratory on the 5500XL SOLiD(TM) platform. The 50 bp single reads were trimmed using Sickle with default parameters.