GSM4680403: WT_18-med-am-RNA; Mus musculus; RNA-Seq
I thought we ended up using FACS sorted AM For the paired-end baseline as well as LPS- and medium-treated replicates of wt- and tg-AM, sequencing libraries were prepared by the DKFZ Genomics and Proteomics Core Facility from total RNA using the SMART-Seq v4 Ultra Low Input RNA Kit according to the manufacturer's instructions. For sequencing, samples were sequenced on a HiSeq 2000 v4, paired-end, 125 bp platform (Illumina). Single-end baseline replicates were prepared by the EMBL Genomics Core Facility from total RNA using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina.  Sequencing was performed on a Next Seq 500, single-end, 75 bp platform.
GEO Accession
GSM4680403