GSM4192664: U2OS_AAVS-LP_C11_800ms_1.5h; Homo sapiens; RNA-Seq
RNA was extracted from each sample using a Trizol/RNeasy Mini Kit (ThermoFisher 15596026, Qiagen 74104). RNA was subjected to SuperScript IV First-Strand Synthesis (Thermo Fisher 18091050) and NEBNext Ultra II Directional RNA Second Strand Synthesis (NEB E7550), according to the manufacturer's instructions. cDNA was then tagmented with Nextera Tn5 (Illumina FC-131-1024) and amplified/indexed by PCR with the NEBNext DNA Library Prep Kit (NEB E6040). Samples were sequenced using a NextSeq 500/550 75 cycle kit (Illumina TG-160-2005).
GEO Accession
GSM4192664