GSM2583646: J-37 2i/L to S/L p23; Mus musculus; RNA-Seq
Undifferentiated ESCs were sorted for SSEA1+ to control for the homogeneity of cells before RNA extraction using the phenol/chloroform protocol. One microgram of total RNA from each sample was used as input for Illumina Ribo-Zero Kit, followed by NEBNext Ultra Directional RNA Library Prep Kit for Illumina. Each paired-end library was prepared with an adaptor with unique index sequence. The size profiles of resulting libraries were than determined on the BioAnalyzer 2100 with Agilent High Sensitivity DNA kit, followed by quantification by qPCR on the Perkin Elmer 7500Fast with Kapa library quantification kit KK4824. These libraries were then pooled together at equal molar concentration and sequenced on an Illumina HiSeq 2000.
GEO Accession
GSM2583646