GSM3098292: NTC_23; Mus musculus; RNA-Seq
The in vivo embryos were produced by superovulating C57BL/6 female mice and mating with DBA2 male mouse. The NTC embryos were manufactured by cumulus cell nuclear transfer and sequential embryos in an in vitro culture. The NTM embryos were manufactured by MEF cell nuclear transfer and sequential embryos in an in vitro culture. Each type of embryo was collected at 6 pronuclear stages, i.e., the 2-cell, 4-cell, 8-cell, morula and blastocyst stages. Each embryo was transferred to lysate buffer in a PCR tube and immediately stored in a -80℃ refrigerator. Embryos were collected from each stage in at least three independent experiments RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM3098292