GSM3938229: GEN1C DDX6_OE_TGFBi_rep1; Homo sapiens; RNA-Seq
RNA isolation was performed using the miRNeasy mini kit (Qiagen). RNA was eluted from the columns using RNase-free water and quantified using a Nanodrop ND-1000. RNA sequencing libraries were prepared using the NEBNext Ultra™ Directional RNA Library Prep Kit for Illumina (Cat#: E7420).   The RNA input for library construction was 50 ng total.  Libraries were amplified for 14 cycles.  Post library constructions, the samples were validated using 2200 Tapestation System and High Sensitivity D1000 ScreenTape kit.  Libraries were quantified using the Kapa Biosystems Library Quantification kit (Catalog Number KK4828) and the BioRad CFX96 instrument.
GEO Accession
GSM3938229