Sequencing of atrap total RNAs
Rosette leaves of 4-week-old the atrap-1 mutant plants were used for RNA extraction by TRIzol reagent (Invitrogen). The RNA was then treated with DNase (Roche) and Ribo-Zero rRNA Removal Kit of Plant (Illumina) and subjected to RNA-seq following "NEBNext Ultra Directional RNA Library Prep Kit for Illumina" (NEB).