GSM3755370: H3.1K36M#2_1_RNA; Homo sapiens; RNA-Seq
Total RNA was extracted using RNeasy plus kit (Qiagen) according to the manufacturer's instructions. The poly-A containing mRNA was purified by poly-T oligo-attached magnetic beads, and then fragmented by divalent cations under elevated temperature. The cleaved RNA was reverse transcript into cDNA with random primers, followed by second strand cDNA synthesis with DNA Polymerase I and RNase H. These single 'A' base added cDNAs were ligated to adapter, amplified and then purified as single strand DNA circle, which was used to generated DNA nanoballs (DNBs) by rolling circle replication PCR. Then DNBs were sequenced with read of 100bp on the BGISEQ-500 platform.
GEO Accession
GSM3755370