RNAseq of Aedes aegypti: wild-type female replicates 2
Two to four day post-emergence adult siblings from the N1 transgenic line were snap frozen in liquid nitrogen and preserved at -80C until the time of extraction. RNA was extracted using the Quick-RNA Miniprep kit (Zymo Research, Irvine, CA). Triplicate RNASeq libraries were prepared for each of the four genotypes using the NEBNext Ultra RNA Library Prep Kit for Illumina with the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs; Ipswich, MA) and multiplexed into 1 lane of a HiSeq 2500