Illumina HiSeq 4000 paired end sequencing; RNA-seq experiment to investigate the effect of inactivating the Apoptosis Antagonizing Transcription Factor (Aatf)
RNA-seq experiment to investigate the effect of inactivating the Apoptosis Antagonizing Transcription Factor (Aatf)
4 week old mice were sacrificed and DBA-positive renal tubules were isolated as previously described (Outeda, P.et al. Kidney international, 92(5), 1130-1144 (2017). & Dafinger, C. et al. Experimental & molecular medicine, 50(6), 75 (2018).). Total RNA was isolated from the DBA enriched fraction by using RNA using phenol-chloroform extraction. All the RNA samples were further treated with DNAse to eliminate genomic DNA contamination as per manufacture's instruction (Quick RNA kit, Zymoresearch). For RNAseq, libraries were prepared using TruSeq stranded mRNA kit (Illumina) according to the provider's protocol.
Experimental Factor: genotype
Aatf homozygous knockout