GSM2757446: Capsella orientalis B (Co B) x Capsella orientalis A (Co A)  Run 1 1; Capsella orientalis; RNA-Seq
For DNA-seq, 300 mg young fresh leaves were used as DNA source. For RNA-seq, dissected endosperm of 300-500 seeds per replicate was used. Endosperm were harvested at 6 DAP and stored at -20oC in RNAlater® (Sigma-Aldrich, St Louis, USA). RNA extraction was done using RNAqueous® Kit with Plant RNA Isolation Aid (Life Technologies, Carlsbad, USA). For methylation analysis, bisulfite coversion was performed on leaves DNA using Epitect Bisulfite Kit (Qiagen) following the manufacturer's instructions. The DNA-sequencing libraries were prepared using TruSeq® DNA Sample Preparation Kit (Illumina, San Diego, USA). The RNA-sequencing libraries were prepared using TruSeq® RNA Sample Preparation Kit v2 (Illumina). The Epitect Bisulfite Converted DNA libraries were prepared using TruSeq® DNA Sample Preparation Kit. All process were done according to the manufacturer's instructions.
GEO Accession
GSM2757446