GSM3100452: GIST_T1_ERCC_DMSO_1; Homo sapiens; RNA-Seq
Total RNA was isolated using an RNeasy Plus Kit (Qiagen). RNA was normalized to cell count, then ERCC RNA Spike-In Control (Ambion) was added to allow for cell count normalization of gene expression changes. Libraries for Illumina NextSeq 500 sequencing were prepared using TruSeq Stranded mRNA Library Prep Kit (Illumina) and equimolar multiplexed libraries were sequenced with single-end 75 bp reads.
GEO Accession
GSM3100452