GSM3755266: Nascent-RNA, Clone #7 Ctl; Homo sapiens; RNA-Seq
Nuclei from two clones per condition were isolated by centrifugation through a sucrose cushion after NP-40 based cell lysis. Nuclei were extracted with 1X NUN (1% NP-40, 1M Urea and 300 mM NaCl). Chromatin was pelleted by centrifugation and washed twice with 1X NUN buffer. Nascent RNAs retained by elongating RNA polymerases were finally isolated by Trizol extraction of the pellets. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM3755266