Illumina HiSeq 2500 paired end sequencing; Isoform-specific depletion of U2AF1
Isoform-specific depletion of U2AF1
Cell lines were grown under standard conditions in DMEM supplemented with 10% (v/v) bovine calf serum (Gibco BRL) PolyA library selection was performed using NEBNext poly(A) mRNA magnetic isolation module (E7490L) according to the manufacturer's specifications.
Experimental Factor: library selection
PolyA+
Experimental Factor: transfection
control