GSM2579424: Mouse wild type erythroid cells H3K27me3 ChIP biological replicate 1; Mus musculus; ChIP-Seq
A single cell suspension was made by gently dissociating the spleen of a phenyhydrazine treated mouse and passing it through a 70um filter. Ter119 selection was performed by staining the cells with phycoerythrin conjugated anti-ter119 antibodies (BD biosciences). The cells were subsequently washed and then incubated with MACS anti-phycoerythrin beads. Cells were then separated using an auto-MACS (Miltenyi Biotec). NEBnext ultra library prep kit
GEO Accession
GSM2579424