Small RNA sequencing from control MCF10A cells
Total RNA was purified using Trizol reagent (Invitrogen) according to the manufacturer’s instructions. Small RNA libraries were created using the TruSeq Small RNA preparation kit (Illumina, RS-200-0012) with variable index primers to allow for multiplexing of samples. The protocol provided by the manufacturer was used to create libraries using 0.5 µg total RNA.