GSM3560338: 0 h_Rep 3_sigJ deletion; Nostoc punctiforme PCC 73102; RNA-Seq
RNA extractions were performed as previously described with a bead beater and phenol (Campbell et al. 2007. Global gene expression patterns of Nostoc punctiforme in steady-state dinitrogen-grown heterocyst containing cultures and at single time points during the differentiation of akinetes and hormogonia. J. Bacteriol 189:5247-5256) Total RNA was ribodepleted using the Ribo-Zero rRNA Removal Kit (Bacteria) (Illumina, Inc.). cDNA was synthesized from the rRNA depleted samples, sheared to a library size of 200 bp, and appended with adapters following standard protocols at the University of California Berkeley QB3 Vincent J. Coates Genomics Sequencing Laboratory .
GEO Accession
GSM3560338