GSM4629088: Pulmonary artery endothelial cells - Input DNA_caMEK5_RepA; Homo sapiens; ChIP-Seq
Cells were cross-linked with 2 mM of DSG (Pierce) for 45 min at RT, washed with PBS followed by cross-linking using 1% formaldehyde for 10 min, quenched with 2 M glycine, washed with PBS after which pellets were snap-frozen and stored at -80°C. Libraries were prepared using standard protocols from the Genomics of Gene Regulation (GGR) project, NHGRI, as performed in the Snyder Lab. Briefly, purified ChIP-DNA samples were end repaired using End-It DNA End Repair Kit (Lucigen) and A-tailed using Klenow Fragment and dATP (New England Biolabs). Illumina TruSeq adapters (Illumina) were ligated using LigaFast (#M8221, Promega) and size-selected by gel extraction before PCR amplification.
GEO Accession
GSM4629088