Reference Transcriptome of P. adspersus
A paired-end library (T0) were generated from RNA samples obtained from muscle of fine flounder, which were subjected to 2 weeks of acclimatization under normal conditions. The library construction was performed using the Illumina kit: TruSeq RNA Sample Prep Kit and following the protocol and suggestions specified by Illumina (v2 TruSeq®RNA Sample Preparation Guide: http://support.illumina.com/sequencing /sequencing_instruments/miseq.html), for the use of its MiSeq sequencing platform. Finally the sequencing was performed using MiSeq 1.8+ of Illumina.