RM0021M
Total RNA was extracted using the PerfectPure RNA Fibrous Tissue Kit (5 PRIME, Hamburg, Germany). We added 4 ul of a 1:100 dilution of either mix 1 or mix 2 of the External RNA Controls Consortium (ERCC) spike-in control RNA (Life Technologies, USA) see associated publication. Total RNA samples were prepared for sequencing using the Illumina TruSeq RNA (Illumina Inc. USA) protocol with the size selection step selecting for ~300 bp fragments and sequenced using the Illumina HiSeq-2000, conducted commercially at Macrogen, South Korea.