GSM4435481: HEK Rot treated Bio 1; Homo sapiens; RNA-Seq
Total RNAs from exposed and control groups  were isolated and quality controlled with protocols from Illumina. A total amount of 2μg RNA per sample was used as input material for library construction. A total of 2ug per sample was used for library construction using the TruSeq Sample Preparation kit from Ilumina (San Diego, CA). Poly-A containing mRNA molecules were isolated from total RNA using oligo-dT attached magnetic beads. Isolated mRNA was then fragmented and synthesized into double stranded cDNA according to kit instructions. Ligation of unique Ilumina adapter indices was completed for each sample before bead purification.
GEO Accession
GSM4435481