GSM3938631: Qb0.3: WTCHG_337410_244142; Mus musculus; ssRNA-seq
Total RNA was extracted from sorted splenic DCs from immunised mice following the manufacturer's protocol (RNeasy Midi Kit, Qiagen), using an on-column DNase-I digest step TruSeq Stranded Total RNA kit (Illumina) Libraries were amplified on a Tetrad (Bio-Rad) using in-house unique dual indexing primers [13]. Individual libraries were normalised using Qubit, and the size profile was analysed on the 2200 or 4200 TapeStation. Individual libraries were normalised and pooled together accordingly
GEO Accession
GSM3938631