RNA-seq of SV40-EGFP-HEK293T: Nanoluc_R2
~1 million cells were washed once with PBS and total RNA extracted using RNeasy purification kit (QIAGEN). DNase treatment was performed directly on column as instructed. NEBNext Ultra II Directional RNA-Seq with Poly-A selection was used to prepare the libraries. All samples were pooled and sequenced on a 75-cycle high output Illumina NextSeq flowcell.