GSM3100807: OreR_ATAC-seq rep3; Drosophila melanogaster; ATAC-seq
Leg cells were lysed in 50 µl Lysis Buffer (10 mM Tris-HCl, pH = 7.5; 10 mM NaCl; 3 mM MgCl2; 0.1 % IGEPAL). Nuclei were collected by centrifugation at 500 g for 5 min. Approximately 60,000 nuclei were suspended in 50 µl Tagmentation Mix [25 µl Buffer (20 mM Tris-CH3COO-, pH = 7.6; 10 mM MgCl2; 20 % Dimethylformamide); 2.5 µl Tn5 Transposase; 22.5 µl H2O] and incubated at 37 °C for 30 min. After addition of 3 µl 2 M NaAC, pH = 5.2 DNA was purified using a QIAGEN MinElute Kit. PCR amplification for library preparation was done for 15 cycles with NEBNext High Fidelity Kit; primers were used according to Buenrostro et al. (2013) DOI:10.1038/NMETH.2688
GEO Accession
GSM3100807