GSM2579164: DOC1-CHD4_ChIP-2; Homo sapiens; ChIP-Seq
Elution buffer (1% SDS, 100 mM NaHCO3) followed by PCR purification kit/columns. Samples were prepared for ChIP sequencing according to the NEXTflex ChIP-Seq kit (Bioo Scientific). Briefly, 10 ng of chromatin was end repaired and enriched for the 300-400 bp fraction using Ampure XP beads (Agencourt). The repaired DNA library was 3’ end adenylated and barcoded Illumina adaptors were ligated followed by bead purification. The adaptor modified library was enriched by PCR for 18 cycles. The ChIP libraries were sequenced according to the Illumina TruSeq Rapid v2 protocol on the HiSeq2500 for a single read 50bp in length and a 7bp index read.
GEO Accession
GSM2579164