GSM2581199: 40E_2_epithelium_LH8; Homo sapiens; RNA-Seq
Endometrial biopsies were collected with endometrial suction Pipelle catheter (Laboratoire CCD, France). Briefly the biopsied tissue samples were dissociated, endometrial stromal cells were stained with fluorocrome-conjugated mouse anti-human CD13 monoclonal antibody (clone TUK1, R-Phycoerythrin, Invitrogen, USA), epithelial cells were stained simultaneously with fluorocrome-conjugated mouse anti-human CD9 monoclonal antibody (clone MEM-61, FITC, Novus Biologicals, USA) and all dead cells were stained with DAPI (Invitrogen, USA).CD9 or CD13 positive and DAPI negative (alive) cells were sorted into a TRIzol Reagent and RNA were extracted immediately after the lysis of cells. Bulk-RNA full transcriptome analysis of FACS sorted endometrial cells was performed by the RNA-seq method, following the single-cell tagged reverse transcription (STRT) protocol with modifications (Krjutškov et al., 2016). 10 ng of high-quality input of cell-type specific RNA was converted to cDNA and amplified to form an Illumina-compatible library. STRT (detailed protocol in PubMed ID 26874359), RNA-seq
GEO Accession
GSM2581199