ChIP-Seq of mouse TH2 cells
Nave CD4+ T cells were activated and polarized in TH2 conditions. On day 1 and 2, T cells were transduced with a retroviral vector expressing TY1-tagged Pparg. On day 4, transduced T cells were harvested for ChIP utilizing a TY1 antibody (Sigma, SAB4800032). ChIP-sequencing libraries were constructed and sequenced (50 bp single end reads) as per standard Illumina protocols.