NextSeq 500 paired end sequencing; RNA-seq on Hesx1+/+ and Hesx1-/- ES cells deprived of 2i/LIF for 24 hours
RNA-seq on Hesx1+/+ and Hesx1-/- ES cells deprived of 2i/LIF for 24 hours
3X Hesx1+/+ and Hesx1-/- ES cell clones were cultured in 2i/LIF conditions for 5 passages. Subsequently, culture medium was deprived of 2i/LIF and cell pellets were collected 24 hours after removal.  Cell pellets from Hesx1+/+ and Hesx1-/- ES cells were processed for total RNA extracion using Trizol reagent (Invitrogen) cDNA library preparation was performed using 250ng of total RNA using the Kapa mRNA library prep kit (Kapa Biosystems) with 12 cycles of PCR
Experimental Factor: genotype
wild type genotype