GSM3391820: T2D9 IP (MeRIP-seq); Homo sapiens; RIP-Seq
Total RNA was extracted from the tissue by TRIzol. mRNA was enriched by polyT beads followed by fragmentation with Bioruptor ultrasonicator. m6A-immunoprecipitation were performed using EpiMark N6-Methyladenosine enrichment kit (NEB cat. E1610S). Kapa RNA hyper kit for Illumina was used to construct library from  mRNA for input RNA and eluted RNA from the  m6A-IP. The libraries were sequenced by the Hiseq4000 platform at SE50 mode. Kapa RNA hyperPrep kit for Illumina
GEO Accession
GSM3391820