GSM4433476: Primary CLL sample2 TLR9lo; Homo sapiens; RNA-Seq
Primary CLL cells were cell sorted and RNA was extracted using an RNeasy mini-prep kit (Qiagen). Total RNA quality and quantity was assessed using an Agilent 2100 Bioanalyser and an RNA Nano 6000 kit (Agilent Technologies). Approximately 100-900ng of Total RNA with an RNA integrity number (RIN) >8 was depleted of ribosomal RNA and used for RNA-seq. RNA libraries were prepared for sequencing using Illumina TruSeq Stranded mRNA Library Prep Kit Following validation, the libraries were normalised to 4nM, pooled together and clustered on the cBot™2 following the manufacturer's recommendations
GEO Accession
GSM4433476