GSM4567273: WT_rep1 [miRNA-seq]; Mus musculus; miRNA-Seq
The rostral-most region of the telencephalic primordium was dissected out from DicerF/F and RxCre-DicerF/F E11.5 embryos, and tissue pieces were immediately frozen. Tissue was then dissociated with Papain (Miltenyi Biotech, 130-092-628) and RNA was extracted using Quick RNA Mini Prep (Zymo Research, R1055). Total RNA (1ug per sample) was used for library preparation and sequencing. Libraries were prepared with NEB Next Small RNA Library Prep Kit (for RNA-Seq) and sequenced on Illumina HISeq 2500 with 75bp single end read.
GEO Accession
GSM4567273