GSM3175495: ATAC-seq mouse E16.5 Cdx2-KO rep1; Mus musculus; ATAC-seq
midguts were dissected and treated in 0.25% Trypsin for 7-10 mins on a vortex station, neutralized with FBS; Epcam+ cells were enriched using magnetic antibody. Whole nuclei were used for chromatin transposition using Nextera Tn5 Transposase (Illumina) The transposed DNA was amplified using NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs)
GEO Accession
GSM3175495