Illumina HiSeq 2500 sequencing; RNAseq of murine myoblast used as cell type control
RNAseq of murine myoblast used as cell type control
Cells isolated from tibialis anterior muscle of 10-16 weeks old male mice, and purified to Integrin7+; VCAM-1; Sca-1- and Lin- progenitors, and placed onto culture dishes for 2-3 days before harvest for total RNA isolation. Total RNA isolated with Trizol and sample produced with Qiagen RNeasy isolation kit Illumina TruSeq Stranded Total RNA Library Prep Human/Mouse/Rat Protocol
Experimental Factor: individual
mouse 3