wellbore biofilm mid-down
Once the DNA sample(s) was(were) received, a qulity test has been done first, then all the qualified DNA is used to construct a library(libraries). For PCR product, the jagged ends of DNA fragment would be converted into blunt ends by using T4 DNA polymerase, Klenow Fragment and T4 Polynucleotide Kinase. Then add an 'A' base to each 3' end to make it easier to add adapters. After all that, fragments too short would be removed by Ampure beads. For genomics DNA, we use fusion primer with dual index and adapters for PCR, fragments too short would beremoved by Ampure beads too. In both cases, only the qualified library can be used for sequencing. The bioinformatics analysis will be carried on with sequencing data.