GSM3174151: B_5: Bone; Mus musculus; RNA-Seq
Tibiae, radii and ulnae were flushed with a needle to remove the marrow. The bones were washed thoroughly with PBS for at least 5 times. Then the bones were minced and digested with type II collagenase at 37°C. Digested tissue was successively filtered through 70-μm and 40-μm cell strainers (BD Biosciences) and then centrifuged with 500g for 5min. The cell pellet was resuspended with PBS. Tomato+ cells were sorted by Bio-Rad S3e and then single cells were picked with fluorescence microscope. Libraries were prepared according to Nextera® XT DNA Library Prep Kit for Illumina  (Cat# FC-131-1096) RNA-seq (SMART-Seq v4 3' DE Kit)
GEO Accession
GSM3174151