Miseq 16S rRNA Sequencing of Porifera Tissue Samples
Genomic DNA was isolated from collected sponge tissues using MOBIO Powerlyzer protocol, RNA samples were converted to cDNA as reverse transcriptase, samples were amplified using PCR using 506 forward and bar-coded 806 reverse primers (targeting the V4 region of 16S rRNA gene), samples were purified and run on an Illumina MiSeq.