GSM4191507: mDRG-2Mp; Mus musculus; RNA-Seq
Human and mouse DRG tissue/cultured cells were stored in RNAlater and frozen in -80 °C until use. Samples obtained at the Washington University at St Louis were shipped to UT Dallas on dry ice for uniform library preparation. All RNA isolation and sequencing was done in the Price Lab. On the day of use, the frozen tubes were thawed to room temperature. To obtain RNA from tissue samples, the tissue was extracted from RNAlater with ethanol cleaned tweezers and put in 1 mL of QIAzol (QIAGEN Inc.) inside 2 mL tissue homogenizing CKMix tubes (Bertin Instruments). To obtain RNA from cell cultures, cells were spun down to the bottom of the tube by centrifuge at 5000 x g for 10 min. RNAlater was then removed from the tube, and cells were resuspended with 1 mL of QIAzol and transferred to the homogenizing tube. For both tissues and cell cultures, homogenization was performed for 3 x 1 min with Minilys personal homogenizer (Bertin Instruments) at 4 °C. This time course was used to avoid heating during homogenization. RNA extraction was performed with RNeasy Plus Universal Mini Kit (QIAGEN Inc.) with the manufacturer provided protocol. RNA was eluted with 30 µL of RNase free water. Stranded single end Illumina Truseq total RNA library Multiplexed libraries were sequenced in a single sequencer run
GEO Accession
GSM4191507