Ion Torrent PGM sequencing of SAMD00060066
"The 16S rRNA genes from each sample were amplified using the following primers: 8F (AGA GTT TGA TCM TGG CCA G) with Ion Torrent adaptor A and sample-specific 8-base tag sequence and 338R (TGC TGC CTC CCG TAG GAG T) with the Ion Torrent trP1 adaptor sequence. PCR amplification was performed using KOD DNA polymerase. Reads were excluded from the analysis if they were shorter than 200 bases, had an average quality score <25 or did not include the correct forward primer sequence or did not include the correct reverse primer sequence (one mismatch was allowed) or had a homopolymer run >6 nt. The remaining reads were assigned to each sample based on the tag sequence."