GSM2420251: mESC_DKOzero_passage2_RRBS; Mus musculus; Bisulfite-Seq
genomic DNA was purified using Phenol/Chloroform extraction Genomic DNA was quantified using a Qubit® 2.0 Fluorometer, and quality-assessed on an Agilient 2200 TapeStation D1000 ScreenTape.  RRBS was performed on 20ng of each sample using the NuGen Ovation® RRBS Methyl-Seq System.  Barcoded samples were pooled in groups of 8 immediately prior to Bisulfite Conversion with the Qiagen EpiTect® Fast Bisulfite Conversion kit.  Library pools were purified with a 1X Agencourt RNA XP bead clean-up and sequenced on an Illumina HiSeq® 2500 Sequencing System. All protocols were carried out according to manufacturer's recommendations except where specified.
GEO Accession
GSM2420251