GSM1416779: P60_wt cones, replicate 2 [RNA-seq]; Mus musculus; RNA-Seq
Cones were isolated by FACS from wild type and C-DGCR8 KO mice at different time points (P30, P40, P50, P60, and P90). RNA extraction was performed with Trizol or Arcturus PicoPure RNA Isolation Kit (Applied Biosystems) according to the manufacturer's instructions. Quantitative and qualitative analysis was performed using 2100 Bioanalyzer. Total RNA from isolated cones (150 ng) was processed using the ScriptSeq Complete (Human/Mouse/Rat) Low Input kit according to the manufacturer's instructions (Epicentre/Illumina). Libraries were pooled equimolarly in batches of 4, and 12 pM of each pool were sequenced on one lane of the HiSeq 2000 instrument using RTA 1.13.48.
GEO Accession
GSM1416779