GSM4193360: 12week_WT_RNAseq; Mus musculus; RNA-Seq
RNA was extracted from intestinal cells using TRIzol followed by column purification (Zymo RNA clean and concentrator column) following the manufacturers' instructions. Total RNA was depleted of rRNA using a Ribo-Zero Gold kit and first strand cDNA was synthesized. Subsequently, second strand cDNA was synthesized, purified, and fragmented. Following purification over a Zymo RNA clean and concentrator column, first strand cDNA was synthesized. Subsequently, second strand cDNA was synthesized, purified, and fragmented. RNA-seq libraries were prepared using Illumina technology. Briefly, end repair, A-tailing, and barcoded adapter ligation followed by PCR amplification and size selection. The integrity of the libraries was confirmed by quBit quantification, fragment analyzer size distribution assessment, and Sanger sequencing of ~10 fragments from each library. Libraries were sequenced using paired-end Illumina sequencing.
GEO Accession
GSM4193360