Evolution of Borrelia burgdorferi sensu lato: Sample PUl
"DNA samples were prepared according to Nextera DNA sample preparation guide (Qiagen). The samples were diluted to 50ng/ul concentration and ""tagmented"" by simultaneously fragmenting DNA using transposomes and adding adapters. After tagmentation samples having adapters on both ends underwent 5 PCR cycles to amplify the product. The sequences were indexed and the resulting libraries were validated using Agilent 2100 Bioanalyser."