muscle under normoxia for 24h
RNA-seq transcriptome libraries were prepared following TruSeqTM RNA sample preparation Kit from Illumina (San Diego, CA), using 5μg of total RNA. Shortly, messenger RNA was isolated with polyA selection by oligo (dT) beads and and fragmented using fragmentation buffer. cDNA synthesis, end repair, A-base addition and ligation of the Illumina-indexed adaptors were performed according to Illumina’s protocol.