GSM3177224: 36-T52-TTR-CII; Homo sapiens; RNA-Seq
Tissue RNA was isolated by RNeasy Kit (Qiagen, Spain) according to the manufacturer's instructions. RNA were quantified by NanoDrop spectrophotometer (NanoDrop Technologies, USA). The purity and integrity of the total RNA were assessed via 2100 Bioanalyzer (Agilent, Germany). All RNA samples analyzed had a RNA Integrity Number ≥ 7. Barcoded RNA sequencing libraries were prepared from 100 ng total RNA using Illumina's TruSeq stranded mRNA kit according to the manufacturer's instructions. Libraries were subjected to paired-end sequencing (101 bp) on a HiSeq-4000 platform (Illumina, San Diego, CA).
GEO Accession
GSM3177224