RNA-Seq of Sus scrofa: alveolar macrophage
Small RNA sample pre kit was used to construct the library. The special structure of 3' and 5' end of smallRNA (complete phosphate group at 5'end and hydroxyl group at 3' end) were used to construct the library. Total RNA was used as the starting sample, and the ends of smallRNA were spliced directly, and then the cDNA was synthesized by reverse transcription. After PCR amplification, the target DNA fragment was separated by PAGE gel electrophoresis, and the cDNA library was obtained by gel cutting.