GSM4506233: Mono-D3-DMSO-2; Mus musculus; RNA-Seq
Scraped out the monolayer using 200ul pipet tip and incubated in 5mM DTT and EDTA–PBS on ice for 30 min, shaking every 10 min. Centrifuged at 800g for 4min. Resuspended the cell with 400ul TrypLE and pipetting 7 times, incubated it for 3min at 37°C. Resuspended the single cells in MACS buffer and then passed through a 40-μm filter.  Count the number of cells after removing dead cells using EasySep Dead cell removal Kit (Stemcell Tech). 10X genomics 3' V2 library construction 10X genomics 3' V2 library construction
GEO Accession
GSM4506233