GSM2770739: stranded_RNA-seq_SETDB1 sgRNA 9 Day 4 Rep-1; Homo sapiens; RNA-Seq
At each time-point, cells were spun down, washed with PBS, flash frozen on dry ice, and RNA was isolated with Qiagen RNeasy micro kits. 100 ng of total RNA, for each sample, was used for library preparation with a TruSeq Stranded Total RNA Library Prep Kit (Illumina). Size of the libraries was confirmed using a 2200 TapeStation and High Sensitivity D1K screen tape (Agilent Technologies) and their concentration was determined via qPCR (Library quantification kit, KAPA
GEO Accession
GSM2770739