Bacterial endophytes and leaf mimicry in Boquila trifoliolata
Genomic DNA was extracted from dried leaves using a CTAB based method, and PCR was performed targeting 16S rRNA V4 region. The sequencing library was prepared by random fragmentation of the DNA or cDNA sample, followed by 5' and 3' adapter ligation. Alternatively, "tagmentation" combines the fragmentation and ligation reactions into a single step that greatly increases the efficiency of the library preparation process. Adapter-ligated fragments are then PCR amplified and gel purified.