GSM4194771: IgG_RIPseq_rep1; Mus musculus; RIP-Seq
Collect the IP product and extract the RNA, and then digesting the RNA with DNase I for 30 min in 37℃ (Thermo, EN0523). Extract the RNA again and reverse transcript RNA using PrimeScript RT Master Mix (Takara RR036A). Ribosomal RNA depleted and strand-specific libraries were constructed with the Ribo-zero gold (Epicentre) and TruSeq Stranded Total RNA Sample Prep kit (Illumina), and the libraries were sequenced using the HighSeq system (lllumina).
GEO Accession
GSM4194771