GSM2425458: a2i_ESC_18_XX_WGBS; Mus musculus; Bisulfite-Seq
PureLink Genomic DNA mini kit (invitrogen) for genomic DNA For whole genome bisulfite sequencing library, 500ng of DNA was sheared by covaris and ligated with methylated adapters supplied by TruSeqTM DNA, Sample Prep Kit-v2 (Illumina). Subsequently, DNA was bisulfite-treated using EZ DNA Methylation-Gold KitTM according to the supplier’s instruction. Final library amplification was performed using Pfu Turbo Cx (Agilent Technologies). The libraries were then sequenced on HiSeq 2500 (2 X 101 bp paired-end reads, Illumina).
GEO Accession
GSM2425458