GSM2842852: input-HEK293-rep2; Homo sapiens; MeDIP-Seq
Total RNA of cells was isolated using RNeasy Mini Kit (Qiagen), and cDNA were sythesised using PrimeScript first strand cDNA synthesis kit (Takara) with oligo-dT. MeDIP-seq were performed as previously described (Xiong et al, Molecular Cell 64, 913-925). Mouse monoclonal anti-5-methylcytidine (BI-MECY-000) for MeDIP was purchased from Eurogentec. The MeDIP-seq libraries were constructed using NEBNext DNA Sample Prep Master Mix (NEB).DNA libraries were subjected to size-selection using AMPure XP beads (Beckman Coulter) and PCR amplification (18-20 cycles for ChIP-seq DNA libraries). The RNA-seq libraries were constructed by BGI, Shenzhen, as the platform BGISEQ-500 instruction described.
GEO Accession
GSM2842852