16S rRNA gene-based analysis of bacterial communities in soil of sample 53
Metagenomic DNA was extracted from paddy soil treated with methane and nitrate (control), replicate D. Subsequently, the V3-V4 region of 16S rRNA genes was amplified. All samples were amplified in triplicate, purified and pooled in equal amounts. Sequences were determined using an Illumina MiSeq sequencer.