GSM2844109: STM ProQ +CL rep1; Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344; RIP-Seq
Bacteria were lysed and the FLAG-tagged proteins was immunoprecipitated using a monoclonal anti-FLAG antibody. The samples were treated with benzonase nuclease, calf intestine phophorylase, and polynucleotide kinase in the presence of radioactive gamma-ATP. Samples were separated with SDS-PAGE and followed by transfer to nitrocellulose membranes. Radioactively labelled RNA-protein complexes were eluted from membranes and treated with Proteinase K. RNA was purified with phenol:chloroform extraction and ethanol precipitation. Purified RNA was used as input for library preparation using the NEB Next Small RNA Library kit according to the manufacturer's instructions.
GEO Accession
GSM2844109