RNA-seq study of vitis vinifera under treatment and control samples, in different time-points
Inflorescences of Vitis Vinifera L. (Thompson Seedless cultivar) were collected from three different experimental conditions: vines sprayed with gibberellic acid (10ppm+12.5ppm+12.5ppm at 20%, 50% and 100% bloom), shaded vines (90%of PAR reduction since 50% bloom) and control (untreated vines). Inflorescences were collected at 3, 5 and 7 days after 100% bloom,deprived from the rachis and frozen in liquid nitrogen. RNA from the powdered samples was extracted and purified using the RNeasy Plant RNA Extraction Kit and RNase-Free DNase Set (Quiagen) following manufacturer’s instructions with one modification, the extraction buffer used. The samples