GSM2585735: homo1 (cryaa-R49C Homo1); Mus musculus; RNA-Seq
Library preparation was performed using a Clontech poly-A kit according to the manufacturer’s protocol. The resulting cDNA was then blunt ended, an A base was added to the 3’ ends, and Illumina sequencing adapters were ligated to the ends. The ligated fragments were then amplified for 12 cycles using primers that incorporated unique index tags and the resulting fragments were sequenced on an Illumina HiSeq 2500 sequencing system using single reads extending 50 bases and targeting 30M reads per sample.
GEO Accession
GSM2585735