sRNA-seq of zea mays: immature ear membrane_bound polysome
After membrane-bound polysome isolation by ultracentrifugation, total RNAs were extracted from the membrane-bound polysomes of maize (Zea mays, inbred line B73) immature ears with TRI Reagent (Molecular Research Center, USA) and used for sRNA library construction according to the manufacturers instructions of NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB, USA).