GSM2843710: JS170745; Mus musculus; RNA-Seq
MEF RNA was harvested with Trizol reagent, followed by chloroform extraction and ethanol precipitation. K562 RNA was harvested with a modified RNeasy protocol as described in the methods section. Chemically treated RNAs were treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in the methods section. MTS-enriched RNAs for transient transcriptome studies were prepared using a modified protocol from Duffy et al. Mol Cell (2015), as described in the methods section. Libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian (Takara Bio USA, cat. 635005).
GEO Accession
GSM2843710