Ion Torrent PGM sequencing; Evaluating bias-reducing protocols for RNA sequencing library preparation
Evaluating bias-reducing protocols for RNA sequencing library preparation
The RNA fragment pool 5' - GCAGUUGCCANNNNNNNNNN  - 3' was synthesised (ThermoScientific Molecular Biology). Fragments contained both 5'phosphate and 3'hydroxyl moieties necessary for ligation. Ligation of 10 ng of RNA pool was performed with the Ion Total RNASeq kit v2 (Life Technologies), following the manufacturer's instructions.
Experimental Factor: protocol type
standard