sRNA-seq of zea mays: seedling shoot total polysomes
After total polysome isolation by ultracentrifugation, total RNAs were extracted from the total polysomes of maize (Zea mays, inbred line B73) seedling shoots with TRI Reagent (Molecular Research Center, USA) and used for sRNA library construction according to the manufacturers instructions of NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB, USA).