Octopus Spring virome - sampled 2007
Thermal water (between 200 and 630 liters) was filtered using a 100 kDa molecular weight cut-off (mwco) tangential flow filter (A/G Technology, Amersham Biosciences, GE Healthcare) and viruses and cells were concentrated to about 2 liters. The resulting concentrates were filtered through a 0.2 m tangential flow filter to remove microbial cells. The viral fractions were further concentrated to about 100 mL using a 100 kDa tangential flow filter and 40 mL of viruses were further concentrated to 400 L and transferred to SM buffer by filtration in a 30 kDa mwco spin filter (Centricon, Millipore). Metagenomic DNA was amplified with an Illustra GenomiPhi V2 DNA amplification kit.