10X genomics sequencing of menidia beryllina
Genomic DNA was adjusted to a concentration of 1.0 ng/ul and 1.25 ng of template gDNA was loaded on a Chromium Genome Chip. Libraries were prepared using Chromium Genome Library & Gel Beak Kit v.2, Chromium Genome Chip Kit v.2, Chromium i7 Multiplex Kit and Chromium controller according to manufacturere's instructures with one modification. Briefly, gDNA was combined with Master Mix, a library of Genome Gel Beads, and partitioning oil to create Gel Bead-in-Emulsions on a Chromium genome Chip. The GEMs were isothermally amplified with primers containing an Illumina Read 1 sequencing primer, a unique 16-bp 10X bar-code and a 6-bp random primer sequence, and bar-coded DNA fragments were recovered for Illumina library construction.