GSM2585892: RIP anti-NONO 10h re-fed (ZT22); Mus musculus; RNA-Seq
Liver was homogenized and ultracentrifuged on a sucrose cushion to isolate liver nuclei. Nuclear lysates were incubated with anti-NONO antibody-coated agarose beads and NONO-bound RNAs were extracted with Trizol reagent and sequenced. Libraries were prepared using Illumina’s TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold according to manufacturer’s instructions
GEO Accession
GSM2585892