GSM2424748: WT mice transplanted with WT bone marrow 4; Mus musculus; RNA-Seq
Total RNA concentrations were determined by a spectrophotometer (NanoDrop 2000, Thermo Scientific, Wilmington, DE), and the quality of the total RNA samples was assessed by an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA).  RNA integrity number (RIN) was ranging from 7.8 to 8.2 in the 8 samples.  mRNA was extracted using a Dynabeads mRNA Purfication Kit (Thermo,Waltham, MA), and cDNA was generated from 500 ng of mRNA using a PrimeScript Double Strand cDNA Synthesis Kit (Takara Bio, Otsu, Japan).  Fifty ng of each cDNA sample was sheared in 200-500 bp using the Covaris S220 (Covaris, Woburn, MA) and 75 bp single-end reads were prepared using KAPA Library Preparation Kits (Kapa Biosystems, Wilmington, MA).  The cDNA libraries were indexed for multiplexing, and were sequenced on the Illumina HiSeq 2500 platform. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM2424748