Sample from Biofilm  of an Anaerobic digester treating municipal solid waste
PCR amplification of  the 16S rRNA gene from the DNA extracted from the biofilm and media samples was accomplished using modified  universal bacterial primer pairs F-(5- CCTACGGGNGGCWG- CAG -3) and  R-(5-GACTACHVGGGTATCTAATCC-3) based on  Klindworth et al. (2013), and an Archaea specific primer pair F-  (5-CCCTAYGGGGYGC-ASCAG-3) and R- (5-GGCCATGCACYWCYTCTC- 3) as described  by Gantner et al. (2011)