GSM4633018: H4T71Gc_mES_LG_ChIP-seq; Mus musculus; ChIP-Seq
ChIP-enriched DNA was obtaind by using ChIP-IT Express Enzymatic (Active Motif, 53009) and H4T71Gc specific antibody (20H3) according to the manufacturer's instructions.  For repairing the ends of DNA fragments isolated by ChIP (50 ng each), adding a single “A” base to the 3'-end of them, and ligating TruSeq DNA adapters (illumina) to them,KAPA hyper Prep kit (KAPA Biosystems) was used according to the manufacturer's instructions. Ligation products were purified on KAPA pure beads (KAPA Biosystems) to remove unligated adapters and subjected to eight cycles of PCR amplification. Completed libraries were quantified using Library Quantification Kit (Takara bio). In this study, all of data processing for ChIP-seq was performed by using Galaxy platform (https://usegalaxy.org/).   ChIP-seq reads were mapped to mm10 assembly of mouse genome or hg38 assembly of human genome using Bowtie2.  The peaks of all samples were detected using MACS. The following parameter was used: input-seq aligned reads as a control file, 25 bp tag size, 300 bp bandwidth, and 10e-03 (for mouse) or 10e-05 (for human) p-value cutoff for peak detection.
GEO Accession
GSM4633018