RNA-seq P.aeruginosa PAO1 +glu 60 min
Ribosomal RNA was depleted from 1 g of total RNA with the RiboZero Gram positive kit (Illumina) according to the manufacturers instructions. Strand specific RNA-Seq libraries were prepared with the ScriptSeqTM v2 RNAseq library preparation kit (Illumina) from 50 ng of rRNA-depleted RNA. The libraries were sequenced on a MiSeq Illumina sequencer; 75 bp reads were produced