GSM4232461: Human1.1; Homo sapiens; RNA-Seq
Briefly, cells, barcoded microparticle beads and lysis buffer were co-flown into a microfluidic device and captured in nanoliter sized droplets. After droplet collection and breakage, the beads were washed, and cDNA synthesis occurred on the bead. Excess oligos were removed by exonuclease I digestion. cDNA amplification was done from a pool of 2,000 bead. Individual PCRs were purified and pooled for library generation. A total of 600 pg of amplified cDNA was used for a Nextera XT library preparation (Illumina).
GEO Accession
GSM4232461