GSM4194463: 360 min-Hi-C-r1; Homo sapiens; Hi-C
Hi-C was performed as previously described (Lieberman-Aiden et al. 2009). Briefly, U2OS cells (2 × 106) were cross-linked with formaldehyde. Chromatin was digested with a restriction enzyme Hind III (NEB), biotinylated with biotin-ATP (Life Technologies), and then ligated with T4 DNA ligase (NEB). DNA was purified and sheared with Covaris LE220 instrument (Covaris). Biotinylated DNA interactions were pulled down with Dynabeads MyOne Streptavin T1 Beads (Life Technologies) and ligated with indexed Illumina Truseq adapters. Amplified libraries were sequenced with Illumina Novoseq platform using the S4 flow cell.
GEO Accession
GSM4194463