GSM1420140: 4C roX2 S2; Drosophila melanogaster; OTHER
4C in S2 cells was carried out as described (Splinter et al., 2012) with minor modifications: 50 million S2 cells, fixed as described above were used. DpnII was used as the primary and Csp6I as the secondary restriction enzyme. 50 million S2 cells were collected and resuspended in 22.5ml of fresh medium. Clone8 cells were washed with PBS and 22.5ml of fresh medium added. 37% formaldehyde solution was added to obtain a final concetration of 1%, followed by incubation at room temperature for 10min. 1.25ml 2.5M glycine solution was added and incubated for another 5 min at RT followed by 10min on ice.
GEO Accession
GSM1420140