Illumina HiSeq 2000 paired end sequencing; RNA-seq comparison: before/after MARIS protocol and library construction technique test
RNA-seq comparison: before/after MARIS protocol and library construction technique test
hESCs were differentiated to Stage 6 cells, INS+ cells comprised approximately 4% of all Stage 6 cells. A large proportion of INS+ cells also co-expressed the pancreatic hormone somatostatin, consistent with previous reports. Also see Hrvatin et al. 10.1073/pnas.1400709111 SI Experimental Procedures. Directed Differentiation. NA The aim of this experiment was to test how well 4 different RNA-seq library construction techniques perform on partially degraded RNA.
Experimental Factor: processing status
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Experimental Factor: library construction
Truseq