GSM1537837: RNA-seq replicate 3 (Control for RIP-seq rep1); Drosophila melanogaster; RNA-Seq
4 x 107 S2 cells were lyzed, and total RNA was purified using TriReagent. Poly(A) RNA was enriched from 40ug total RNA using Poly(A)Purist MAG kit (Ambion) according to manufacturer’s specifications. Library was performed as described in (Kielpinski et al. 2013, Deep Sequencing Data Analysis). 28 cycles of PCR was used to create the final library See individual experiment
GEO Accession
GSM1537837