GSM4633078: WT.Cont.rep2; Mus musculus; RNA-Seq
Total RNA was extracted using Direct-zol RNA MiniPrep kit (Zymo Research, R2052) according to manufacturer's instructions. TruSeq Stranded mRNA Library Kit (Cat# 20020595, Illumina, San Diego, CA, USA) and the TruSeq RNA UD Indexes (Cat# 20022371, Illumina, San Diego, CA, USA) were used for library preparation Libraries were sequenced Paired-End 51 bases (in addition: 8 bases for index 1 and 8 bases for index 2) using the NovaSeq 6000 instrument (Illumina) and the SP Flow-Cell loaded at a final concentration in Flow-Lane of 2pM and including 1% PhiX.
GEO Accession
GSM4633078