RNA-Seq of Folsomia candida: 4 Days Bravo500 Treatment - Replicate2
Springtails were exposed to the EC50 (calculated through the reproduction test, following guideline 11267 (ISO 1999) ), using Bravo500 (plus control) and sampled at different time-points (2, 4, 7, and 10 days). Four biological replicates per treatment (Biosample) were used for RNA extraction, each consisting of approximately 75 organisms. Total RNA was extracted following the TRIZOL Protocol and, after purification steps, was used to prepare Illumina TruSeq RNA sequencing libraries according to manufacturers instructions. These files represent the raw reads prior to the trimming procedures described in the associated manuscript with the corresponding bioproject.