GSM4681810: HV_5; Homo sapiens; RNA-Seq
Total RNA was extracted by Trizol reagent. RNA quality and concentration were measured by using an Agilent 2100 Bioanalyzer (Agilent Technologies, USA). Samples with sufficient amount of high-quality RNA (No contaminants, RNA integrity number > 5) were used for transcriptome library preparation. mRNA was isolated using oligo (dT) beads (Thermo Fisher Scientific, USA) and fragmented using NEBNext Ultra RNA Library PrepKit for Illumina (New England Biolabs, USA) according to the manufacturer's instructions.
GEO Accession
GSM4681810