Paired end reads used to assemble Asterina pectinifera de novo testis transcriptome
Whole testis was dissected from gravid males and put in Trizol. RNA was extracted and then cleaned with a Qiagen RNeasy Micro column with on-column DNA digestion. The sequencing libraries were prepared with Illumina reagents, (TruSeq Sample Prep Kit) with the maximum recommended RNA input. The protocol was followed exactly with the addition of a gel selection step, (Caliper LabChip XT) prior to PCR amplification.