GSM3244102: Oga(Vav-Cre) 3; Mus musculus; RNA-Seq
Bone marrow cells collected from 3 OgaVav-Cre and 3 wildtype female mice were sorted for Lin-Sca+Kit+ cells using a BD FACSAria Fusion Flow cytometer and the following antibodies: Lineage Cocktail FITC (R&D Biosystems), Sca-1(D7)APC (ebiosciences), c-Kit (2B8)PE (ebiosciences), FVD (ebiosciences). At least 2x10^4 cells were collected for each sample. RNA was extracted using RNeasy plus micro kit (Qiagen) following manufacturers protocol RNA libraries were prepped using NEBNext Ultra RNA Library Prep Kit for Illumina and the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs).
GEO Accession
GSM3244102