GSM1637665: Plus_NusA_5; Bacillus subtilis; RNA-Seq
RNA was isolated from frozen pellets using the RNeasy kit (Qiagen), residual DNA was removed by DNase treatment. Total RNA was dephosphorylated (CIP) and treated with Ribo-Zero™ kit for rRNA removal. A small RNA-oligo was ligated to 3’ end of the RNA and illumina libraries were generated using TruSeq® stranded mRNA library kit.
GEO Accession
GSM1637665