WGS of bioreactor sludge metagenome: one-phase partitioning airlift bioreactor for VOC removal
Genomic DNA was extracted using E.Z.N.A.? Soil DNA Kit (Omega Bio-tek, Inc., USA) according to the manufacturer. The DNA was quantified by Nanodrop spectrophotometer (NanoDrop Technologies, USA). The PCR products were pooled and purified using the QIAquick Gel Extraction Kit (Qiagen, Germany). DNA library was conducted on the Illumina HiSeq2000 platform by applying the 101bp paired-end strategy.