GSM2152799: ZH11D-OsbZIP23; Oryza sativa Japonica Group; ChIP-Seq
Seedlings were immediately cross-linking after harvest by 1% formaldehyde under vacuum. 3g of seedlings for each sample at the four-leaf stage were used for chromatin isolation. Isolated chromatin was sheared to approximately 100-500 bp by Diagenode® Bioruptor. For ChIP-Seq, the DNA was immunoprecipitated by anti-OsbZIP23 antibody, and the precipitated DNA was purified and dissolved in distilled water. The eluted ChIP DNA was used to generate Illumina sequencing libraries following the manufacturer's protocol. The eluted DNA from ChIP assay was used to generate Illumina sequencing libraries following the manufacturer's protocol.
GEO Accession
GSM2152799