GSM2585965: CPL4RNAi_rep3; Arabidopsis thaliana; RNA-Seq
Seedlings were flash frozen immediately after harvesting and subjected to total RNA extraction using RNeasy Plant Mini kit (QIAGEN). The total RNA was submitted to Otogenetics Corporation (Norcross, GA) for RNA-seq analysis (paired-end, 100-bp). After validating the integrity and purity of total RNA using Agilent Bioanalyzer and OD260/280, 2 µg of total RNA was used for rRNA depletion using Ribo-Zero™ Plant Leaf RZPL1224 and Ribo-Zero™ Magnetic Plant Leaf MRZPL1224/MRZPL116t (EpiCentre). Recovered total RNA from rRNA depletion was subjected for cDNA synthesis using SMARTer PCR cDNA Synthesis kit (Clontech Laboratories, Inc., Mountain View, CA USA, catalog# 634925).
GEO Accession
GSM2585965