RNA-seq of Phalaenopsis aphrodite 4 DAI protocorm treated with 1 % sucrose
Mature seeds were collected from the capsules of Phalaenopsis aphrodite Rchb.f, and were further grown in medium containing 1/2 MS plus 1% (w/v) sucrose for 4 days. RNA were extracted from sample collected as described above. Both 2 g RNA samples were treated with DNase I, and then sequenced by using Solexa/Illumina RNA-seq (Illumina Hiseq 2000 platform, BGI Tech Solutions).