WGS of p31KO hepatocytes; 2months old male: Sample hepatocytes_WGS
Amplified DNA (1 µg) was sonicated using Covaris S2 ultrasonicator and libraries then prepared with the KAPA High throughput Library Preparation Kit (KK8234, KAPA Biosystmes). Samples are end repaired, adenylated at the 3’ ends, and barcoded with multiplex Illumina adapters. PCR amplified libraries (6 cycles) are purified with AmpureXP beads, and then validated on the Agilient 2100 Bioanalyzer. 