16s Rag mouse R1.6 day 3
16S rRNA gene amplification and sequencing were performed at the genomics facility at Instituto Gulbenkian de Ciencia (Portugal). Briefly, DNA was extracted from fecal samples with a QIAamp DNA Stool Mini Kit (QIAGEN). Samples were amplified using primers specific to the V3-V4 region of the 16s rRNA gene and pair-end sequenced on an Illumina MiSeq Benchtop Sequencer.