Transcriptome
Sequencing libraries were prepared from poly(A)+ RNA as described previously (He and Jiao, 2014). RNAs from seedlings and inflorescences were mixed with a 1:1 ratio. Total RNAs and polysomal RNAs were submitted to Poly(A)+ RNAs purification by two rounds of hybridization to oligo (dT)-coated Dynal magnetic beads (Life Technology) separately. Sequencing libraries prepared from poly (A)+ RNA were constructed using mRNA Library Prep Master Mix Set (NEB) according to the manufacturer’s instructions. Libraries were sequenced as paired-end 100-mers using HiSeq 2000 (Illumina) with standard settings.