GSM2155520: Dynamics experiment 3: 72h; Danio rerio; OTHER
TRIzol extraction GFP sequences were amplified by PCR with primers complementary to GFP, including an 8 bp barcode sequence and adapter sequences for Illumina sequencing. Samples were then pooled and subjected to magnetic bead cleanup (AMPure XP beads – Beckman Coulter). Finally, sequenceable libraries were generated by a second round of PCR with indexed primers from Illumina’s TruSeq Small RNA Sample Prep Kit.
GEO Accession
GSM2155520