27 SMRT cells of Drosophila santomea STO CAGO 1482 Gen 14 PacBio RS II reads
High Molecular Weight DNA extraction with QIAgen Blood and Tissue kit (Genomic-tip 100/G), sheared with 24 gauge blunt-end needle (Chakraborty et al. 2015 BiorXiv), verified length distribution by FIGE, submitted 140 ug across 5 library preparations using BluePippin 15-80kb size selection, standard PacBio 20kb library preparation without Covaris G-tube step, followed by final BluePippin 15-80kb size selection of SMRTbell templates. Sequenced 27 SMRT cells, totalling ~15.2 Gb. The library prep resulted in 4 runs of the PacBio RS II sequencer: a Titration run (R153) of 2 SMRT cells (to determine optimal loading concentration), a Main run (R154) of 7 SMRT cells at the optimal loading concentration determined by the titration run, a second Titration run (R159) of 2 SMRT cells to confirm the previous optimum, and a second Main run (R162) of 16 SMRT cells. R153-E01.1 and R159-B02.1 were loaded at 400 pM, and the rest at 300 pM.