GSM1313979: Twin pair A Case MeDIP-seq; Homo sapiens; MeDIP-Seq
DNA was extracted from whole blood using a salt extraction method MeDIP-Sequencing was performed by BGI at its Shenzhan laboratory. Extracted DNA was fragmented using a Covaris sonication system and sequencing libraries were prepared from 5µg fragmented gDNA. End repair, <A> base addition and adaptor ligation steps were performed using Illumina's Single-End DNA Sample Prep kit. Adaptor-ligated DNA was immunoprecipitated by anti-5mC using a commercial antibody (Diagenode). MeDIP DNA was purified with ZYMO DNA Clean & Concentrator-5 columns, and amplified using adaptor-mediated PCR. DNA fragments between 220 and 320 bp in size were gel-excised.
GEO Accession
GSM1313979