454 RNA-SEQ sequencing library for ovarian/placental tissues from Poeciliopsis prolifica
RNA was isolated from the ovarian/placental tissue of 13 pregnant Poeciliopsis prolifica individuals using Qiagen RNeasy kit. All fertilized eggs and developing embryos with belly-sacs were dissected out of tissues before RNA isolation. RNA was then subjected to Poly-A selection and sequencing library construction was done using resulting Poly-A selected RNA and standard 454 cDNA library construction protocols