GSM1312742: HGDP01029; Homo sapiens; RNA-Seq
Total RNA was extracted from lymphoblastoid using an RNeasy Mini Kit (Qiagen). mRNAs were purified using magnetic oligo-dT beads and randomly fragmented to 300-400 nucleotides in length. First-strand cDNA synthesis was performed using random hexamers and reverse transcriptase. This was followed second-strand cDNA synthesis with dUTP via the dUTP strand-marking protocol. Illumina TruSeq adaptors were ligated to the ends of the double-stranded cDNA fragments followed by digestion with uracil N-glycosylase (UNG) to remove second strand cDNA. A 300-400 bp size-selection of the final product was performed by gel-excision, following the Illumina-recommended protocol. Libraries were paired-end sequenced with an average insert size of ~200bp
GEO Accession
GSM1312742