2bRAD of Prairie Voles
Genome-wide 2bRAD-seq is based on the use of type IIB restriction enzymes that target a small fraction of the genome. Type IIB enzymes excise 36bp-fragments alongside the recognition site to allow detection of genetic variants. Vole DNA was treated with the enzyme BcgI (NEB) to produce sufficient genome fragmentation; fragments were ligated to Illumina adaptors, amplified for 10 cycles, and then purified according to the protocol designed by Wang and collaborators (2012).