GSM4633111: H3K27ac_RevFloxed_Control; Mus musculus; OTHER
H3K27ac (Cell Signaling Technology #8173) or Rabbit IgG (Cell Signaling Technology #3900) at 1:100 was incubated with cardiomyocytes which were attached to Concanavalin A coated magnetics beads (Bangs Laboratories #BP531) overnight at 4 degrees. Next day, 2.5ul of pA-MNase from 1:10 dilution of the original stock provided by the Henikoff lab was applied to cell-beads mixture and incubated at room temperature for 10min. Pull-down DNA was extracted in phase-lock tubes using phenol-chloroform method. DNA library was prepared using Hyper-prep kit (KAPA Biosystems KK8502).
GEO Accession
GSM4633111