GSM3758146: 2A; Homo sapiens; RNA-Seq
TriZol extraction (TRI Reagent, Molecular Research Center, Cincinnati, OH) The SMARTer® Ultra® Low Input RNA kit (Takara, Mountain View, CA) was used to generate cDNA from 9 ng of total RNA. Using the ThruPLEX® DNA-seq kit (Takara), 1-2 ng of double-stranded cDNA was end-repaired and A-tailed to prepare for adaptor ligation. Indexed adaptors were ligated to sample DNA, and the adaptor-ligated DNA library quality was assessed by measuring nanomolar concentration and the fragment size in base pairs. Sequencing was performed on the NextSeq 500 unit (Illumina, San Diego, CA)