GSM3258344: 7-sp-C4; Mus musculus; RNA-Seq
ARC tissues in the coronal brain sections were accurately cut with syringe needles under the microscope and homogenized immediately in the TRIZOL reagent (Invitrogen). Total RNA was separated using chloroform and precipitated with isopropanol. 1~2 μg total RNA was used to prepare the sequencing library in the following steps: 1) Total RNA is enriched by oligo(dT) magnetic beads (rRNA removed); 2) RNA-seq library preparation using KAPA Stranded RNA-Seq Library Prep Kit (Illumina), which incorporates dUTP into the second cDNA strand and renders the RNA-seq library strand-specific. The completed libraries were qualified with Agilent 2100 Bioanalyzer and quantified by absolute quantification qPCR method. To sequence the libraries on the Illumina HiSeq 4000 instrument, the barcoded libraries were mixed, denatured to single stranded DNA in NaOH, captured on Illumina flow cell, amplified in situ, and subsequently sequenced for 150 cycles for both ends on Illumina HiSeq instrument.
GEO Accession
GSM3258344