GSM2424843: 12-23-15-No-Dox_S123.1; Mus musculus; RNA-Seq
For RNA-seq, total RNA was prepared using RNEasy micro kit (Qiagen) and analyzed on RNA pico bioanalyzer chips (Agilent) prior to library construction. Strand-specific RNA-seq libraries were generated for each sample from 100 ng of total RNA using the Kapa Stranded RNA-seq kit (Kapa Biosystems, Cape Town, South Africa) after poly-A enrichment according to the instructions of the manufacturer.  The consistent fragment lengths of the sequencing libraries were ascertained using a Bioanalyzer 2100 micro-capillary gel electrophoresis instrument (Agilent, Santa Clara, CA).  The barcoded libraries were quantified by fluorometry on a Qubit instrument (LifeTechnologies, Carlsbad, CA), and pooled in equimolar ratios. The pool was quantified by qPCR with a Kapa Library Quant kit (Kapa Biosystems) and sequenced on one lane of an Illumina HiSeq 4000 sequencer (Illumina, San Diego, CA) run with single-end 90 bp reads.
GEO Accession
GSM2424843