GSM4568667: Control #3; Mus musculus; RNA-Seq
Treated bone marrow-derived macrophages were lyzed in TRIzol (Thermo Fisher), and RNA was extracted according to the manufacturer's instructions, followed by RNA purification using a RNeasy Plus Mini Kit (Qiagen). DNA library was constructed with a TruSeq mRNA Stranded Kit (Illumina). The RNA from each step and library DNA quality were analyzed with RNA Nano assay chips, RNA Pico assay chips, and DNA Nano assay chips using a 2100 bioanalyer (Agilent). Library was subjected to next-generation sequencing analysis in a high-throughput sequencing center with a HiSeq2500 at the Children's Hospital of Philadelphia/Beijing Genomics Institute core facility (CHOP/BGI).
GEO Accession
GSM4568667