GSM2585888: RIP anti-NONO fasted (ZT10); Mus musculus; RNA-Seq
Liver was homogenized and ultracentrifuged on a sucrose cushion to isolate liver nuclei. Nuclear lysates were incubated with anti-NONO antibody-coated agarose beads and NONO-bound RNAs were extracted with Trizol reagent and sequenced. Libraries were prepared using Illumina’s TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold according to manufacturer’s instructions
GEO Accession
GSM2585888