Profiling the endogenous function of USP7 in AR transcriptional regulation in a global scale
After transfection with USP7 siRNA for 72 h, prostate cancer cells were LNCaP exposed to 10 nM androgen, dihydrotestosterone (DHT) for 16 h and subjected to RAN extraction. Total RNA was isolated using Trizol (Invitrogen) and Nucleospin RNA II (Macherey-Nagel). One microgram of mRNA was used for library preparation using Illumina TruSeq RNA sample Preparation v2 kit (TruSeq).