16S-Illumina of bacterial profiles: maternal and infant faecal specimens
Nucleic acid was extracted from maternal and infant faecal specimens participating in the Drakenstein Child Health Study using the QIAsymphony DSP Virus/Pathogen Mini Kit. The V4 region of the 16S rRNA gene was amplified using published primers. Products were cleaned and pooled at 100ng. Gel extraction of the pooled products was performed, followed by purification. The library was quantified using the KAPA qPCR quantification kit and the Agilent DNA 1000 kit