GSM3758138: P19_1-3_BV; Mus musculus; RNA-Seq
Mouse skin blood endothelial cells and lymphatic endothelial cells were purified from CD1 wildtype mice at postnatal day 19. Each sample was pooled from 3 different mice FACS-isolated cells were directly lysed into TRIzol LS (Invitrogen). Total RNA was purified using the Direct-zol RNA MiniPrep kit (Zymo Research) per manufacturer's instructions. Quality of the RNA for sequencing was determined using an Agilent 2100 Bioanalyzer; all samples used had RNA integrity numbers >8. Library preparation using the Illumina TrueSeq mRNA sample preparation kit was performed at the Weill Cornell Medical College Genomic Core facility (New York), and RNAs were paired-end sequenced on Illumina HiSeq 4000 machines.
GEO Accession
GSM3758138