GSM4005540: A. terreus_48h; Aspergillus terreus; RNA-Seq
Fungal mycelia were homogenize and then RNA was extracted and centrifuged to pellet the RNA. Barcoded RNA libraries were prepared using Lexogen's Quant-Seq 3' mRNA seq kit for Ion Torrent. This approach generated the libraries of the sequences close to the 3' end of the polyadenylated RNAs and only one fragment was produced per transcript. The RNA input was quantified by the spectrophotometry or the fluorometry. Approximately 300-500 ng of the RNA input was required for the library generation. The modified Quant-Seq protocol was performed as suggested for the low input, partially degraded RNA. The External RNA Controls Consortium (ERCC) obtained from Life Technologies were used to spike into each of the prepared library reactions at the manufacturer's recommended concentration. The library quantification and quality control were performed using high sensitivity DNA kit and Agilent 2100 Bioanalyzer.
GEO Accession
GSM4005540