GSM1314049: GM02456; Homo sapiens; Bisulfite-Seq
Genomic DNA was extracted from cell pellets using the DNeasy Blood and Tissue Kit (Qiagen) according to manufacturer’s protocol. We constructed genomic DNA libraries using the TruSeq Sample Preparation kit (Illumina Inc.) following the Illumina standard protocol: the ends of the fragments were repaired, an adenine was added at the 3′ extremities of the fragments and Illumina TruSeq adapters were ligated at each extremity.  After adaptor ligation, we treated DNA with sodium bisulfite using the EZ DNA Methylation Kit (Zymo Research, Irvine, CA, USA) according to manufacturer’s protocol.
GEO Accession
GSM1314049