GSM2425370: Control mTS; Mus musculus; RNA-Seq
Total RNA from the whole cell-extract was isolated using RNeasy Mini Kit (#74104) from Qiagen per the manufacturer’s protocol with on column DNase digestion.  RNA concentrations were quantified using a NanoDrop Spectrophotometer at a  wavelength of 260nm. Integrity of the total RNA samples was evaluated using an Agilent 2100 Bioanalyzer (Agilent Technologies Inc., Santa Clara, CA) and the samples with RNA integrity values above 7.0 were used for the sequencing experiments. Total RNA was used to construct RNA-seq libraries using the Illumina TruSeq RNA Sample Preparation Kit v3 according to manufacturer's instructions
GEO Accession
GSM2425370