GSM2844582: DES-exposed myometrial progenitor cells; Rattus norvegicus; RNA-Seq
RNeasy RNA isolation kit (Qiagen, Valencia, CA) was used to isolate RNA for RNA-seq analysis. RNA samples were treated with DNase I and the RNA purity and quality was checked by Bioanalyzer prior to cDNA synthesis. cDNA libraries were constructed using SPRI-works Fragment Library System I (Beckman Coulter, Brea, CA), which were then PCR-enriched and purified. Ten pM DNA was loaded into the paired end flow cell on cBOT for cluster generation and thereafter loaded on the HiSeq2500 platform (Illumina Inc.,San Diego, CA) to generate single 36 bp sequence reads.
GEO Accession
GSM2844582