GSM2232096: L095; Homo sapiens; RNA-Seq
Total RNA was prepared from surgical resected samples using Qiagen RNeasy Mini kit after homogenizing with Mixer Mill MM400 (Qiagen). The quality of RNA was assessed with RNA integrity number (RIN) using Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA), for the selection of available samples for NGS analysis (RIN>7.0). mRNA were captured using Dynabeads® mRNA DIRECT™ Micro Purification Kit (Thermo Fisher Scientific, Waltham, MA) and used to generate sequencing libraries of barcoded fragment using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific) following the manufacture’s instructions. Libraries were sequenced on the Ion Proton™ System by four libraries per Ion PI™ Chip v2, using Ion PI™ Template OT2 200 kit v3 and Ion PI™ Sequencing 200 kit v3 (Thermo Fisher Scientific).
GEO Accession
GSM2232096