GSM1534600: gonad_1; Caenorhabditis elegans; RNA-Seq
Total RNA isolation was performed using the PicoPure extraction kit following the manufacturer’s instructions. All RNA samples were eluted from columns with 11 µl DEPC treated water. Then all samples were processed with the RIBO-ZERO magnetic gold kit (Epicentre) according to the manufacturer’s protocol to remove rRNA. The resultant rRNA-free supernatant was concentrated using the RNA clean and concentrator kit (Zymo research). Library preparation was performed using the Ovation® RNA-Seq System V2 kit (Nugen) according to the manufacturer's protocol
GEO Accession
GSM1534600