GSM4633181: WT_IgG_CnR_rep1; Homo sapiens; OTHER
We performed anti-TFAP2A and anti-MITF Cleavage Under Targets and Release Using Nuclease (CUT&RUN) sequencing in wild-type, MITF mutant and TFAP2A; TFAP2C double mutant SkMel28 cell lines essentially as described (Skene and Henikoff 2017). CUT&RUN libraries were prepared using the KATA Hyper Prep Kit (Roche). Quality control post-library amplification was conducted using the 2100 Bioanalyzer for fragment analysis. Libraries were pooled to equimolar concentrations and sequenced with paired-end 150 bp reads on an Illumina NovaSeq 6000 instrument.
GEO Accession
GSM4633181