RNA-seq TRIP culture: phenanthrene
The TRIP culture was grown with phenanthrene as substrate and sulfate as electron acceptor. Cells were harvested in the mid-exponential phase by centrifugation (20 min, 6000 x g, 4 C). Total RNA was immediately extracted using the TrizolTM reagent (Invitrogen). Samples were treated with the DNA-freeTM kit (Invitrogen) to remove DNA. After rRNA depletion, the mRNA was used for strand-specific library construction and sequenced on the Hiseq 4000 platform (PE100).