Illumina MiSeq sequencing; qiita_ptid_1656:10485.A1419
DNA extracted from bacteria pelleted from milk sample
Illumina MiSeq 515fbc, 806r amplification of 16SrRNA V4
barcode
CATCTGGA
center_name
UC Davis Genome Center
center_project_name
Raw_and_Partially_Proccesed_California_Bovine_Milk
linker
GT
pcr_cond
initial denaturation:94degC_3min; denaturation:94degC_45sec; annealing:54degC_1min; elongation: 72degC_30sec; final elongation:72degC_10min; 35total cycles
pcr_primers
FWD:GTGTGCCAGCMGCCGCGGTAA REV:GGACTACHVGGGTWTCTAAT
primer
GTGTGCCAGCMGCCGCGGTAA
reverseprimer
GGACTACHVGGGTWTCTAAT
run_center
UC Davis Genome Center
run_date
2/21/15
run_prefix
4_reads
samp_store_dur_renamed
12 - 24 hour
samp_store_temp
4 degree Celsius
samp_vol_we_dna_ext
30 mL
sequencing_meth
Sequencing by synthesis
target_gene
16S rRNA
target_subfragment
V4
temp_renamed
41