Marburgvirus Strain Angola Deep Sequencing Human Thp1 Cells 6hpi
Deep RNA sequencing of Human Thp1 infected cells with Marburg virus                 Strain Angola at multiplicity of infection of 3. RNA was extracted with TriZol,                 poly-A RNA purified with Invitrogen Dynal oligo dT magnetic beads and then RNA-Seq                 libraries created using NEB Next mRNA-Seq kit using 18 cycles of PCR. Samples                 sequenced on Illumina HiSeq 2500, using 100nt single read                 settings..