Deep sequencing of small RNA of Giardia Trophozoite
sRNAs ranging from 18 to 30 nt were gel-purified and ligated to the 3' adaptor (5'-pUCGUAUGCCGUCUUCUGCUUGidT-3'; p, phosphate; idT, inverted deoxythymidine) and 5’ adaptor (5’-GUUCAGAGUUCUACAGUCCGACGAUC-3’). Ligation products were gel-purified, reverse transcribed, and amplified using Illumina’s sRNA primer set (5’-CAAGCAGAAGACG GCATACGA-3’; 5’-AATGATACGGCGACCACCGA-3’).