GSM2769629: Fix_Control_3; Mus musculus; RNA-Seq
Rneasy FFPE kit 1ng RNA was reverse-transcribed with the SMARTseq v4 Ultra Low Input RNA kit for Sequencing (Clontech), which uses oligo(dT) priming and sheared with a Covaris S220. End repair, multiplexed adaptor ligation, and library amplification (13-15 cycles) were performed using the Ovation Ultralow Library System V2 1-96 (NuGEN). Libraries were sequenced (101 bp, paired-end) at the Stanford Functional Genomics Facility with a HiSeq 4000 (Illumina).
GEO Accession
GSM2769629