RNA-seq Microlepia sp.
Isolation of RNA was carried out as previously described . Briefly, total RNA was isolated using the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany), followed by DNase treatment (Fermentas, St Leon-Rot, Germany), according to the manufacturers´ protocols. Quality of RNA was determined utilizing Nanodrop 1000 (Peqlab, Germany) and Bioanalyzer 2100 (Agilent). Only samples which passed common quality criteria(OD 260/280 ratio around 2.0; no degradation) were further processed.