RNA-seq of sus scrofa:PBMCs
Library preparation included RNA extraction, cDNA synthesis and PCR amplification.RNA was extracted from PBMCs and cDNA was synthesized. IgH libraries were amplified by multiplex PCR with a universal forward primer specific for IgH variable (V) gene segments and reverse primers specific for IgH constant (C) gene segments. Qualified libraries were index separated, ligated with sequencing adaptors, pooled together and sequenced by an Illumina Miseq pair-end platform.