NGS of influenza virus A/WSN/33 PA-K289A-P1 genomic RNA
Reverse-transcription and amplification of the 8 viral genomic segments were performed as described10.  The amplification products were purified using a Gel and PCR Cleanup kit (Macherey Nagel). NGS was performed by the PIBnet platform at Institut Pasteur, using the Nextera XT DNA Library Preparation kit (Illumina).