sRNA-seq of oryza sativa japonica group: immature panicle membrane_bound polysome
After membrane-bound polysome isolation by ultracentrifugation, total RNAs were extracted from the membrane-bound polysomes of rice (Oryza sative japonica group, cv. Nipponbare) immature panicles with TRI Reagent (Molecular Research Center, USA) and used for sRNA library construction according to the manufacturers instructions of NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB, USA).