GSM2844557: C3; Drosophila melanogaster; RNA-Seq
The brains from third instar wandering larvae were extracted in ice cold Dulbecco's buffered saline solution (Sigma) and brain lobes were removed. Twenty five ventral nerve cords for each biological replicate were transferred into RNAlaterTM reagent (Qiagen) and stored at 4C. Total RNA was extracted using RNAzol RT(Sigma) following manufacturer protocol. Sample quality control was performed using the Agilent 2100 Bioanalyzer. Qualifying samples were then prepped following the standard protocol for the TruSeq stranded mRNA library kit (Illumina) on the Illumina Neoprep automated nanofluidic library prep instrument. Sequencing was performed on the Illumina NextSeq 500 with Paired End 42bp × 42bp reads.
GEO Accession
GSM2844557