GSM1419603: 35S-SUC2-Input-rep2; Arabidopsis thaliana; ChIP-Seq
The ChIP-seq was performed as previously described (Wierzbicki et al., 2008). In brief, 3 g of 14-day-old seedlings was collected, placed in 35 ml of 1% formaldehyde PBS buffer, and then fixed by vacuum for 2 min followed by another 8 min. After glycine was added, samples were subjected to a vacuum for 5 min to stop the fixation. Samples were washed three times with sterile water and then ground into powder with liquid nitrogen. Nuclei were extracted with Honda buffer. The nuclei solution was sonicated to break genomic DNA into ~500-bp fragments. After centrifugation, supernatants were removed and used for immuno-precipitation. Dynabeads (Invitrogen, cat# 10003D) were used for pre-clearing and antibody binding. The antibodies were anti-MYC(Millipore, cat#05-724). The libraries were prepared using the NuGen Ovation Ultralow Library Systems and were sequenced in the Genomics Core Facilities of the Shanghai Center for Plant Stress Biology, SIBS, CAS (Shanghai, China) with Illumina HiSeq2500.
GEO Accession
GSM1419603