Bacterial population of 7 day Kombucha tea biofilm
Primers needed for amplification of the 16S V3 region were designed and synthesized and then build a library and sequenced in Miseq.  The primers used for PCR amplification had tailed Illumina adaptors. Where appropriate the PCR strategy was adapted to generate the library. The community DNA acted as template for amplification. Libraries from each sample were tracked using a sequence barcode: CGATGT which was incorporated in the libraries generated.