GSM4233283: scRNA Mouse Spleen Untreated; Mus musculus; RNA-Seq
Spleens were isolated and smashed through a 70uM sterile filter to obtain single cell suspensions. Red blood cells (RBCs) were lysed using ACK lysing buffer. Cells were resuspended in RPMI supplemented with 10% FBS and 1% Pen/Strep. For human samples, cells were isolated from whole blood of healthy volunteers using ErythroClear RBC depletion kit (STEMCELL). Cells were resuspended in RPMI supplemented with 10% FBS and 1% Pen/Strep. Cells were treated with recombinant interferon for 4 hours as indicated. Isolated cells were encapsulated using microfluidics technology, and barcoded using a unique molecular identifier (UMI). scRNAseq data were generated on Illumina HiSeq 3000 instruments according to the manufacturer's instructions.
GEO Accession
GSM4233283