s170956B-sh2
Fresh cells were harvested, and resuspended in 1 x phosphate buffer saline (PBS) with 0.04% bovine serum albumin. Then cells were lysed and subsequently RNA was reverse-transcribed and converted into cDNA libraries for RNA-Sequencing (RNA-Seq) analysis using a Chromium Controller and a Chromium Single Cell 3 v2 Reagent kit (10 x Genomics) following the manufacturers protocol. Finally, sequencing was performed on the Illumina Hiseq X Ten sequencer with a sequencing depth of at least 100,000 reads per cell and 150 bp paired-end reads (PE150). Alignment of reads to the genome and generation of gene counts per cell was performed by Cell Ranger 1.2 software (10 x Genomics).