GSM1641096: Wildtype_mRNAseq_2; Mus musculus; RNA-Seq
Testes were stripped of the tunica albuginea, placed in TRIzol (Invitrogen), homogenized, and stored at −20°C. Total RNAs were prepared according to the manufacturer's protocol. Total RNAs were then DNase-treated using DNA Free Turbo (Ambion) Libraries were prepared using the Illumina mRNA-Seq Sample Preparation Kit (San Diego, CA) according to the manufacturer's protocol. Libraries were validated with an Agilent Bioanalyzer (Santa Clara, CA). Libraries were diluted to 10 pM and applied to an Illumina flow cell using the Illumina Cluster Station.
GEO Accession
GSM1641096