GSM2842801: NRAS metastatic melanoma ChIP-FOSL2 JQ1 (-) [SKmel147]; Homo sapiens; ChIP-Seq
For FOSL2 and TEAD4, 10x10^6 cells, were crosslinked with 0.25M DSG for 45min, followed by 1% PFA crosslinking for 10 min at RT. ChIP was performed essentially as previously described (Tian et al., Two-step Crosslinking for Analysis of Protein-chroamtin Interactions. Methods Mol Biol, 2012. 809:105-120. Roe, J.S., et al., BET Bromodomain Inhibition Suppresses the Function of Hematopoietic Transcription Factors in Acute Myeloid Leukemia. Mol Cell, 2015. 58(6): p. 1028-39). Libraries for ChIP-seq were done as previously described (Hasson D., et al., The octamer is the major form of CENP-A nucleosomes at human centromeres. Nat Struct Mol Biol. 2013 Jun;20(6):687-95).
GEO Accession
GSM2842801