Dactylopius transcriptome
Half a milligram of frozen adult female Dactylopius coccus, obtained from Lanzarote Spain, were ground into a fine powder with a mortar and pestle under liquid nitrogen. Total RNA was subsequently extracted using the RNeasy mini kit (Qiagen) according to manufacturers instructions. Polyadenylated RNA was converted into cDNA with an oligo-dT primer and a reverse transcriptase (RT˛ Easy First Strand Kit, Qiagen). The cDNA samples were sequenced with a total yield of 5GB/sample (corresponding to 55 million 90-bp reads) by BGI-Shenzhen, China using 90-bp paired-end Illumina sequencing technology