GSM4006651: SH-SY5Y cells, DCC-IP-3; Homo sapiens; RIP-Seq
The lysate of SH-SY5Y cells was incubated with magnetic Dynabeads pre-coupled with antibodies (mouse-anti-DCC (BD Biosciences, 554223); rabbit-anti-Nrp1 (Abcam, ab81321)) for 1.5 hours at 4°C. Beads were then washed 3 times and processed for RNA isolation. Immunopurified RNAs are reverse-transcribed, and then amplified by a PCR-based method (Tang et al., Nat Methods 6, 2009).