GSM3758201: Zn minus root-rep1; Oryza sativa; RNA-Seq
Rice shoots and roots were collected after Zn-deficiency and Zn-resupply treatments.  Eighteen samples (Zn-plus shoot, Zn-minus shoot, Zn-resupply shoot, Zn-plus root, Zn-minus root, Zn-resupply root, each with three biological replicates) were harvested for small RNA library construction and sequencing. Roots and shoots from three rice seedlings were collected and mixed together as one sample. Total RNAs were extracted from samples using Trizol reagent (Invitrogen, Carlsbad, CA). Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA), and the quantity and the purity were analyzed by Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA). The RIN value (RNA integrity number) of all RNA samples were more than 8.0. Ten µg of total RNA of each sample was subjected to isolate Poly (A) mRNA with poly-T oligoattached magnetic beads (Invitrogen, Carlsbad, CA). After purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature. The cleaved RNA fragments were then reverse-transcribed to create cDNA libraries according to the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA). The average insert size for the paired-end libraries was 300 bp (±50 bp). The paired-end sequencing was performed on an Illumina Hiseq4000 at the LC-BIO (Hangzhou, China).
GEO Accession
GSM3758201