GSM2231274: H4K16ac N2 L3 rep3; Caenorhabditis elegans; ChIP-Seq
The pellet was resuspended in one volume of FA buffer (50 mM HEPES/KOH pH 7.5, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate; 150 mM NaCl) plus protease and phosphatase inhibitors. To obtain chromatin fragments lysate was sonicated on high, for 30 seconds on 30 seconds off, two times for 10 minutes and one time for 15 minutes. 50 ul of the lystate was used to make IP input library. 3 ug of anti-H4K16ac (Millipore 07-329) was added to 400 ul of lysate (containing approximately 50 ug DNA) for ChIP. ChIP DNA were ligated to Illumina adaptors and amplified by PCR. Library DNA between 200-550 bp in size.
GEO Accession
GSM2231274