CFIAFB20200089_L. monocytogenes
Brain  Heart Infusion broth was inoculated with a single colony of bacteria and  incubated for 14 to 16 h at 35C. Genomic DNA was isolated from 400 L of  culture using the Maxwell 16 Cell SEV DNA Purification kit (Promega,  Madison, WI). DNA was then quantified using the Quant-iT High-Sensitivity  DNA Assay Kit (Life Technologies Inc., Burlington, ON). Sequencing libraries  were constructed from 1 ng of gDNA using Nextera XT DNA Sample Preparation  and Index Kits (Illumina, Inc., San Diego, CA) according to manufacturers  instructions. Genomic sequencing was performed on the Illumina MiSeq Platform  (Illumina, Inc.) using a 600-cycle MiSeq Reagent kit v3 (Illumina, Inc.).