Paired End RNASeq for RWK-NIL pollen - Replicate 1
At least 200 mg of fresh pollen was collected between 9:00-9:30am from three biological replicates of RWK, RWK-NIL, NP2222, and NP2222-HI lines, snap frozen on liquid nitrogen, and shipped to Data2Bio for RNA-seq analysis. Pollen samples were ground in liquid nitrogen using an RNase-free mortar and pestle. The RNeasy Plant Mini Kit from Qiagen was used for RNA extraction. Lysis buffer was added to the ground pollen followed by additional grinding at RT. Once fully ground, the sample was processed according to the RNeasy extraction protocol. RNA was checked for quality using an Agilent RNA Nano kit. One RNA-Seq library was constructed for each sample