GSM4508594: Old fly brain - rep 1; Drosophila melanogaster; RNA-Seq
After 5 days (young) and 70 days (old), brains were dissected and RNA was purified with TRIzol. PolyA+ RNA was purified using Dynabeads Oligo(dT)25 (Thermo Fisher) beads and constructed into strand-specific libraries using the dUTP method . UTP-marked cDNA was end-repaired using end-repair mix (Enzymatics, MA), tailed with deoxyadenine using Klenow exo- (Enzymatics), and ligated to custom dual-indexed adapters with T4 DNA ligase (Enzymatics). Libraries were size-selected with SPRIselect beads (Beckman Coulter, CA) and quantified by qPCR before and after amplification. Sequencing was performed in paired-end mode on a NextSeq 500 (Illumina, CA).
GEO Accession
GSM4508594