re-seq of B73
Total genomic DNA (~2g) was fragmented into 200500bp pieces by sonication in a Bioruptor. The fragmented DNA was end repaired, Subsequently Proceeding 3-end adenylation, and adapter ligation. ~0.5g adapter-ligated DNA (200500 bp) was took out to be amplified by 6 cycles of PCR using Q5 HiFi HotStart and subject to purification using VAHTS DNA Clean Beads (Vazyme). Library quality was checked using NGS3K to ensure that the libraries size is in the right range (200-700bp with a peak around 300bp). For each genotype, all libraries passed quality control and sequenced across multiple lanes on a NovaSeq 6000 machine.