GSM4681845: TgBAC(dbx1b:GFP) positive cells 1; Danio rerio; RNA-Seq
14hpf zebrafish embryos were dissected to remove the brain and tail region. The trunk region is dissociated by mechanical pipetting in FACSMax solution. The dissociated cells are resuspended in PBS, FACS sorted for GFP positive cells. The sorted cells are spun down and the pellet was flash frozen in Trizol for RNA extraction. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM4681845