16S rRNA gene sequencing of methanogenic enrichment culture: oilfield produced water
16S rRNA gene fragments from a 25 mL aliquot of produced water enriched with heavy oil (for 8 months) were amplified using universal PCR primers 926F and 1392R. Nextera XT i7 and i5 adapters were added to purified amplicons using a second round of PCR. The final purified and normalized amplicons were sequenced using a MiSeq Reagent Kit v3. Raw forward and reverse sequence reads were merged in QIIME.