GSM4232499: Huh-7 treated with cinobufagin for 24 h-2; Homo sapiens; RNA-Seq
Add 0.2ml chloroform to 1ml TRIzol, vibrate violently for 15 seconds, leave at room temperature for 3 minutes, and centrifuge at 10000×g for 15 minutes. The aqueous phase is transferred to the new tube, and the organic phase can be retained if DNA and protein are separated. The RNA in the aqueous phase was precipitated with isopropanol. Add 0.5ml isopropanol to 1ml TRIzol and leave at room temperature for 10 minutes. After centrifugation at 2 -- 8℃10000×g for 10 minutes, no RNA precipitation was observed before centrifugation, and gelatinous precipitation appeared on the side and bottom of the tube after centrifugation. Remove the supernatant. The RNA precipitate was washed with 75% ethanol. Add at least 1ml 75% ethanol to 1ml TRIzol. Centrifuge at 2-8℃ for 5 min at no more than 7500×g, discard supernatant. Leave to dry or vacuum dry the RNA precipitate at room temperature for about 5-10 minutes. Do not vacuum centrifuge dry, too dry will lead to the dissolution of RNA greatly reduced. Add 25-200 l of RNase free water, smack it several times with the nozzle, and place it at 55-60 ° c for 10 minutes to dissolve the RNA. RNA libraries were prepared for sequencing using  oligo -dT beads
GEO Accession
GSM4232499