Agave Yeast Candida apicola genome
Stranded the mRNA TruSeq Library Preparation Kit (Illumina, RS-122-2101) was used following the protocol of Low Throughput (LT) to prepare RNA libraries Seq. RNA extraction was done using Qiagen RNAeasy kit. The spheroplasts (cells without cell wall) to extract the RNA was done with the help of Glass beads, acid washed Sigma (G8722-100G) with a diameter of 425-600 um