GSM3258351: day 3 rMEFs_RNA-seq; Mus musculus; RNA-Seq
Total RNA was isolated from the cells at day 0, day 3 and day 6 post-OSKM induction and the fully reprogrammed iPSCs using Trizol RNA extraction reagent (ThermoFisher), following the manufacturer's protocol. The concentration and purity of total RNA were assessed on Nanodrop 2000 (ThermoFisher Scientific, MA, USA), and the integrity of total RNA was evaluated with 2 % agarose gel electrophoresis and RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). The cDNA libraries of long chain RNAs (mRNA/lncRNA/circRNA) and small RNAs were generated from 10 μg and 2 μg of total RNA, respectively. rRNA was removed using Epicentre Ribo-zero rRNA Kit (Epicentre, USA). Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (#E7530L, NEB, USA) and NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (#E7300L, NEB, USA), respectively.
GEO Accession
GSM3258351