GSM4630095: pooled_input_6h; Mus musculus; ChIP-Seq
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. Chromatin was purified by phenol-chloroform extraction and ethanol precipitation. Illumina sequencing libraries were prepared from the ChIP and input DNA by the standard consecutive enzymatic steps of end-polishing, dA-addition, and adaptor ligation. After a final PCR amplification step, the resulting DNA libraries were quantified and sequenced on Illumina's NextSeq 500 (75 nt reads, single end).
GEO Accession
GSM4630095