GSM2231370: IonXpress_RNA_046; Mus musculus; RNA-Seq
RNAs were extracted using PureLink RNA Mini kit by Life Tech 1ug of total RNA was enriched for Poly A RNA using Ambion Dynabeads mRNA Direct Micro kit and barcoded libraries for sequencing were prepared using the Life Technologies RNAseq V2 kit for Ion Torrent according to manufacturer’s standard protocol. Libraries were amplified 14 cycles and the quality of each library was checked on an Agilent Bioanalyzer DNA High Sensitivity chip. A 1ul aliquot of each sample was pooled and run on an Ion Torrent PGM 314 chip. The libraries were equally pooled based on PGM 314 read data, purified on a Pippin Prep gel, quantified by the Agilent Bioanalyzer and sequenced on an Ion Torrent Proton sequencer.
GEO Accession
GSM2231370