GSM4633210: Control plasmid_1st; Homo sapiens; RNA-Seq
Total RNA was extracted with Trizol according to the manufacturer's instructions, the RNA was further purified with two phenol-chloroform treatments and then treated with RQ1 DNase to remove DNA. To assess the quantity and quality of RNA, absorbance at 260 nm/280 nm was detected by Agilent Bioanalyzer 2100. Kit (Vazyme). Polyadenylated mRNAs were purified and fragmented, and then converted into double strand cDNA. After the step of end repair and A tailing, the DNAs were ligated to VAHTS RNA Adapters (Vazyme). Purified ligation products corresponding to 200-500 bps were digested with heat-labile UDG, and the single strand cDNA was amplified, purified, quantified and stored at -80℃ before sequencing. For high-throughput sequencing, the libraries were prepared following the manufacturer's instructions and applied to Illumina HiSeq X Ten system for 150 nt paired-end sequencing.
GEO Accession
GSM4633210