De novo sequencing and analysis of anemone coronaria transcriptome
High quality RNA samples from five healthy plants and from five diseased plants were pooled to create: healthy (1S) and infected (2I) libraries, respectively. About 10 μg of RNA of each library were sent to Eurofins MWG-Operon (Ebersberg, Germany – http://www.eurofinsdna.com/home.html) for c-DNA preparation of two 3’-fragment cDNA libraries and pyrosequencing.