GSM2838514: WT zebrafish embryo (60% epiboly, Rep 2); Danio rerio; RNA-Seq
Embryos were dechorionated and deyolked manually, mechanically dissociated, washed and resuspended in PBS + 0.1% BSA + 20% Optiprep, filtered through a cell sieve, and encapsulated using a standard Drop-seq apparatus. Lysis occurred in droplets in Drop-seq lysis buffer (). Libraries were prepared according to Drop-seq Protocol v3.1 (12/28/2015), with 12–13 cycles of PCR amplification and 50–100 STAMPs per PCR reaction.
GEO Accession
GSM2838514