GSM3244481: CA12_3MC_2 (chip-seq); Homo sapiens; ChIP-Seq
Cells were pelleted at 300g for 5 minutes at 4C, resuspended in ice cold phosphate buffered saline and pelleted again.  RNA was extracted using the miRNeasy RNA extraction kit. ChIP DNAs were extracted as described previously (PMID: 29268714). ChIP-seq libraries were prepared according to manufacturer's instructions in the ThruPLEX® DNA-seq Kit V2 (Rubicon Genomics, Ann Arbor, MI). The libraries were sequenced to 51 base pairs from both ends on an Illumina HiSeq 4000 following the manufacturer's protocols.
GEO Accession
GSM3244481