16S rRNA sequencing. Control sterile seawater 72h Replicate 1
DNA was extracted using the DNeasy PowerSoil Kit. Bacterial communities were characterized by sequencing of amplicons for the 16S rRNA gene. Fragments of approximately 500 base pairs (bp) were amplified by PCR using the primer pair 341Fw/785Rv (V3-V4 hypervariable region). 341F: 5-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG-3, 785R: 5-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC-3. The primers contained the following MiSeq adapters: 5-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-3, 5-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACA-3. PCR products were sequenced using Illumina MiSeq technology (2 x 300 bp) at the Ramaciotti Centre for Genomics (UNSW, Sydney, Australia).