WGS of Heliothis virescens single pupa (PacBio2)
Genomic DNA from 4 pupae, one of which was also used for Illumina PE sequencing, were prepared into libraries for PacBio sequencing. For each library, the SMRTbell Template Preparation Kit version 1.0 (Pacific Biosciences, Menlo Park, CA, U.S.A.) was used for gDNA preparation, but shearing and size-selection steps differed. For this library, shearing prior to DNA concentration was avoided to maximize gDNA fragment length, and a BluePippin (Sage Science Inc., Beverly, MA,U.S.A.) was used to select fragments that were at least 7kb long. This produced sufficient prepared library material for 5 SMRTcells. Prior to sequencing, the library concentration and fragment length profiles were checked on a Qubit 2.0 and an Agilent Tapestation 2200 (Agilent Technologies, USA) with a high molecular weight tape. Sequenced at the University of North Carolina Chapel Hill Sequencing facility on a PacBio RS II.