amplicon: CRISPR5 array, Pfu WT JFW02 strain with plasmid pYS3-Pslp
DNA was isolated from cultured Pyrococcus furiosus that had been transformed with plasmid pYS3-Pslp.  A region of the CRISPR5 array corresponding to newly acquired spacers was amplified from total DNA by PCR.  Illumina adapters were added through additional rounds of PCR.