GSM2231338: 491; Mus musculus; RNA-Seq
Gastrocnemius muscles were carefully removed, visibly cleared of any adipose or connective tissue and immediately flash frozen in liquid nitrogen. RNA was extracted using TRIzol reagent and column purified using a PureLink RNA mini kit. Sample quality was determined using an Agilent bioanalyzer. Sequencing libraries were prepared from 1 ug of the purified RNA extract. 500ng of total RNA was enriched for Poly (A) RNA and then barcoded libraries for sequencing were prepared using the Lexogen mRNA Sense kit for Ion Torrent according to manufacturer’s standard protocol. Libraries were amplified 13 cycles and the quality of each library was checked on an Agilent Bioanalyzer DNA High Sensitivity chip. A 1ul aliquot of each sample was pooled and run on the Ion Torrent PGM 314 chip. The libraries were then pooled based on read counts from the PGM 314 chip, purified on a Pippin Prep gel, quantified by the Agilent Bioanalyzer and sequenced on an ABI 5500 Wildfire sequencer.
GEO Accession
GSM2231338