Taf4b-deficient mouse oocyte RNA-seq - Mouse #2
We collected oocytes from Taf4b-deficient and wild-type oviducts after ovulation, mechanically and enzymatically stripped of all granulosa cells, and processed the cells for cDNA synthesis and amplification for sequencing using the WTA kit from Sigma-Aldrich. We used a custom sequencing primer as previously described (Reich, A., Klatsky, P., Carson, S., & Wessel, G. (2011) JBC, 286(47), 40743-40749.) and after sequencing, removed the first 9 bases from each 50 bp read.