RNA-seq of Bordetella pertussis BPSMT733M
strain were cultivated in SS media to OD600 nm = 2. total RNA was extracted using TriReagent (Invitrogen). Genomic DNA was removed by two steps of DNase I treatment (Sigma Aldrich). Total RNA was treated with the Ribo-Zero rRNA Removal Kits (Illumina) following the manufacturer's recommendations. The rRNA-depleted RNA were then used to prepare the Illumina librarie using the TruSeq RNA Library Preparation Kit (Illumina) following the manufacturer's recommendations