GSM3757258: AL (Apis laboriosa) transcriptome; Apis laboriosa; RNA-Seq
For each species, total RNA was isolated from each of the three samples, respectively, using the TRIzol reagent (Invitrogen, CA, USA). After quantifying the concentration of obtained RNA by Qubit® 2.0 Flurometer (Life Technologies, CA, USA), a single pooled RNA sample was prepared for each species by mixing equal amount of the RNA (1 μg) extracted from each of the three samples. The pooled 3 μg of RNA sample was used as input material for the species to construct sequencing libraries, which were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations.
GEO Accession
GSM3757258