GSM4568699: Q_2; Caenorhabditis elegans; RNA-Seq
mRNA extraction, barcoding, reverse transcription and in vitro transcription were performed according to the CEL-seq protocol (Hashimshony et al., 2012) using the MessageAmp II kit (Ambion). Illumina sequencing libraries were prepared according to the CEL-seq2 protocol (Hashimshony et al., 2016) using the SuperScript® II Double-Stranded cDNA Synthesis Kit (Thermofisher), Agencourt AMPure XP beads (Beckman Coulter), and randomhexRT for converting aRNA to cDNA using random priming.
GEO Accession
GSM4568699