Illumina MiSeq sequencing; qiita_ptid_51:1714.McG.LA1020
For the current study, we collected soils from a primary forest at Pasoh Forest Reserve, regenerating forest that had been logged 50 years ago, and an oil palm agricultural site that has been in cultivation for 25 years. In each habitat type, three plots were delineated (20 m X 20 m) and five soil cores were composited as a representative sample for each plot. The ITS region of fungal DNA has already been sequenced using the Illumina platform. All samples were extracted with the EMP protocol.
EMP V4 515f,806rbc protocol
barcode
GGTAAGTTTGAC
center_name
ANL
center_project_name
mcguire_malaysiapasohlanduse_soil
illumina_technology
MiSeq
linker
GT
pcr_primers
FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
primer
GTGTGCCAGCMGCCGCGGTAA
run_center
CCME
run_date
2/14/12
run_prefix
McGuire_S0_L001
samp_size
.1,g
sample_center
McGuire Lab, New York NY
sequencing_meth
sequencing by synthesis
target_gene
16S rRNA
target_subfragment
V4