microbiome of Aphelenchus avenae
Aphelenchus avenae was cultured on MEA agar plates that were pre-seeded with Botrytis cinerea as a food. After two weeks, nematodes were isolated by Baermann funneling under sterile conditions. Nematodes were ground in liquid nitrogen with pellet pestle grinder in 1.5 ml sterile eppendorf tubes. Thereafter, the total (nematode+bacteria) genomic DNA was isolated with E.Z.N.A. Mullusc DNA Kit  based on manufacturers instruction. V4 region of the bacterial 16S rRNA gene was amplified and sequenced with Illumina Miseq platform.