Metagenome sequencing of Massospora platypediae conidial plug from infected cicada (Platypedia putnami)
Fungal genomic DNA was extracted from harvested condial plugs from infected cicada. These plugs were processed aseptically, with half of the visible fungal plug removed with a sterile scalpel andtransferred to a 1.5 ml microcentrifuge tube for DNA extraction. DNA was extracted using Wizard kit (Promega, Madison, WI, USA) and constructed into Truseq gDNA Illumina libraries following manufacturer directions and sequenced on Illumina HiSeq 4000 in 2x150 format.