GSM4232538: WTA rep4; Mus musculus; RNA-Seq
Total RNA was prepared following standard protocols. Briefly, each frozen sample was placed into a tube containing a 5-mm stainless steel bead. Working on ice, 1 mL Trizol reagent (Invitrogen, Groningen, The Netherlands) was added, and homogenization was performed twice in a TissueLyser (Qiagen) at 30 Hz for 3 minutes. Samples for RNA-sequencing analysis were then processed using the RNeasy Mini Kit (Qiagen, 74104) according to manufacturer instructions. Libraries were generated from 500 ng of total RNA using TruSeq Stranded mRNA Sample Preparation kit according to Illumina user guide (PN 15031047) with the following modifications: 2 minutes mRNA fragmentation and 12 cycles library PCR amplification.
GEO Accession
GSM4232538