GSM4629884: EA 947__32 VitD; Homo sapiens; RNA-Seq
Around 100–200 ng of total RNA was used to prepare RNA-Seq libraries using the TruSeq RNA Sample Prep Kit (Illumina, CA, USA), following the protocol described by the manufacturer. High-throughput sequencing (HTS) was performed using an Illumina HiSeq2500 with each sample sequenced to a minimum depth of ∼ 50 million reads. To prepare RNA-Seq libraries using the SMART-Seq® v4 RNA-seq kit (Clontech Laboratories, Mountain View, CA), 10 pg–10 ng of total RNA was used following the protocol described by the manufacturer. mRNA libraries were prepared using Illumina's TruSeq™ RNA Sample Prep Kit v2 as described by the manufacturer
GEO Accession
GSM4629884