GSM1537830: iCLIP replicate 2; Drosophila melanogaster; RIP-Seq
5 x 107 S2 cells were crosslinked  250mJ/cm2 at 254nm. 0,005U/µl  ribonuclease T1 was used to trim RNA prior to IP with anti-Imp antibody.  The RNA purification and library generation was performed as described in (Konig et al. 2011, Journal of visualized experiments :JoVE). 21 cycles of PCR was used to create the library. See individual experiment
GEO Accession
GSM1537830