Metagenomic sample from denitrifying Microbial fuel cell: Autotrophic denitrifying Microbes
The amplicon libraries were prepared using Nextera XT Index Kit (Illumina inc.) as per the 16S Metagenomic Sequencing Library preparation protocol (Part # 15044223 Rev. B). Primers for the amplification of the V3-V4 hyper-variable region (Table-1) of 16S rDNA gene of bacteria and Archaea were designed in Xcelris NGS Bioinformatics Lab. These primers were synthesized in Xcelris PrimeX facility. The libraries were prepared from both the samples after amplifying the V3-V4 region of the 16S segment. Size of libraries were 642 bp and 629 bp for Sample A and Sample B respectively. The libraries were sequenced using the illumina sequencing chemistry to generate ~150 Mb of data per sample.