WGS of Heliothis virescens single pupa (PacBio1)
Genomic DNA from 4 pupae, one of which was also used for Illumina PE sequencing, were prepared into libraries for PacBio sequencing. For each library, the SMRTbell Template Preparation Kit version 1.0 (Pacific Biosciences, Menlo Park, CA, U.S.A.) was used for gDNA preparation, but shearing and size-selection steps differed. For the this library, shearing was minimal and no size selection was performed. This produced sufficient prepared library material for 17 SMRTcells. Prior to sequencing, the library concentration and fragment length profiles were checked on a Qubit 2.0 and an Agilent Tapestation 2200 (Agilent Technologies, USA) with a high molecular weight tape. Sequenced at the University of North Carolina Chapel Hill Sequencing facility on a PacBio RS II.