16S rRNA gene sequences of thermophilic anaerobic digester: before increase of organic loading rate from 0.75 to 1.13 gVS/(L*day) (day 178 of experiment)
Two PCR reactions performed in triplicate. First step amplified the 16S rRNA genes and the second one attached Illumina-compatible barcodes and adapters. This was performed with universal primer sets 515'F/805R.