Transcriptome Sequencing profilings of Fagopyrum tataricum Leaf in response to Pb stress
cDNA libraries were constructed following the Illumina manufacturer's instructions supplied with the Truseq? RNA sample prep Kit (Illumina,San Diego, USA). Briefly, mRNAs were purified from the total RNA using Dynabeads Oligo (dT)25 (Life Technologies). The poly (A) mRNA was purified from 25 ¦Ìg of pooled total RNA using Oligo (dT) magnetic beads, and cut into short fragments of about 200 bp in fragmentation buffer. The derived mRNAs were fragmented and reverse transcribed into first-strand cDNAs with random hexamer primers and then the second-strand cDNAs were further synthesized. The double-stranded cDNAs were purified and ligated to adaptors for Illumina paired-end sequencing. After agarose gel electrophoresis, suitable fragments were chosen for PCR amplification to create final cDNA libraries.