GSM2422404: TotRNA rep3; Solanum tuberosum; RNA-Seq
Mitochondrial and total RNA were extracted using Tri Reagent® (Molecular Research Center) according to the manufacter’s instructions. For total RNA, the protocol was slightly modified to eliminate starch. RNA were then DNase-treated with DNase RQ1 (Promega). Cytosolic mRNAs were purified from RNA extracts using oligo-dT magnetic beads. The use of oligo-dT allowed the elimination of many mitochondrial mRNAs, because stable mitochondrial mRNAs lack polyA tails. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM2422404