GSM1314621: Human Skin Control 1; Homo sapiens; RNA-Seq
Skin: total RNA was extracted using a combination of the phenol extraction method and column purification. Briefly, samples were homogenised in Trizol (Invitrogen), mixed with chloroform and spun so that the aqueous liquid phase containing the nucleic acids could be removed. This solution was then placed on Qiagen RNeasy columns and subjected to numerous wash steps to purify the RNA sample. DRG: , total RNA from DRG samples was extracted using the miRNeasy kit (Qiagen). All samples were subjected to on-column DNase digestion (Qiagen) to prevent genomic contamination and subsequently analysed using an RNA 6000 nano chip (Agilent Technologies) to confirm sufficient integrity. cDNA libraries were prepared using the TruSeqTM RNA Sample Preparation Kit (Illumina), low throughput protocol. 200 ng of total RNA were subjected to poly(A) enrichment using poly(T)-attached magnetic beads. Poly(A)-enriched RNA was subsequently used for reverse transcription and library preparation according to Illumina’s instructions
GEO Accession
GSM1314621