Illumina HiSeq 2500 sequencing; RNA-seq of human hepatocellular carcinoma cell line Huh7 and immortalized hepatocyte cell line THLE5B treated with experimental compound AD80, Sorafenib, and vehicle for 24 hours
RNA-seq of human hepatocellular carcinoma cell line Huh7 and immortalized hepatocyte cell line THLE5B treated with experimental compound AD80, Sorafenib, and vehicle for 24 hours
HUH7 and THLE5B cells were plated in 6 well plates, treated for 24 hrs with 0.1% DMSO, 5 µM sorafenib, and 50 nM AD80. Cells were collected in “RNAlater”, a RNA storage buffer RNA was extracted using the Qiagen RNeasy mini kit. mRNA TruSeq Stranded, 175bp. Standard protocol
Experimental Factor: cell line
HuH-7
Experimental Factor: compound
AD80
Experimental Factor: dose
0.05