GSM3177490: Lung_DM3; Mus musculus; RNA-Seq
Tissues were harvested following perfusion and submersed whole in Invitrogen RNAlater Solutions (Invitrogen). Samples were flash frozen in -80o C until further processing. mRNA from all tissues was purified using RNeasy mini kit (Qiagen) according to manufacturer's guidelines. RNA quality was assessed by TapeStation (Agilent). Libraries were prepared using a derivation of MARS-seq as described previously 85, optimized for bulk RNA-seq. Briefly, 50 ng of RNA from each sample were reverse transcribed into cDNA, pooled and linearly amplified by in vitro transcription. Following fragmentation and addition of Illumina sequences, successful final libraries were sequenced using the Illumina NextSeq-500 platform. 3' end mRNA libraries were prepared for sequencing using a method developed at Dr. Ido Amit's lab, Weizmann Institute of Science
GEO Accession
GSM3177490