GSM1639588: E11.0_T1_2610; Mus musculus; RNA-Seq
E11 AGM region (41-45 sp): ECs: CD31+VE-cadherin+CD41-CD43-CD45-Ter119-, T1 pre-HSCs: CD31+CD45-CD41lowc-Kit+CD201high, T1 CD201-: CD31+CD45-CD41lowc-Kit+CD201low/-, T2 pre-HSCs: CD31+CD45+c-Kit+CD201high, T2 CD41low: CD31+CD45+CD41low; fetal liver: E12 HSCs: Lin-Sca-1+Mac-1lowCD201+, E14 HSCs: CD45+CD150+CD48-CD201+; bonemarrow: Adult HSC: ESLAM(CD45+ EPCR+ CD48- CD150+). FACS enriched cells were kept on ice until lysed and reverse transcribed. Morphologically deformed cells were discarded. Single cells were rinsed in PBS-BSA, and manually transferred into cell lysis buffer with a mouth pipette. 0.05 μl of 1:200,000 dilution of ERCC RNA spike-in Mix1 (Ambion) were added to lysis buffer per reaction. The cDNA libraries from single cells or 10-cell pools were generated as described previously (Nature methods, 2009). 50-200 ng amplified single-cell or 10-cell-pool cDNA were sonicated to ~250 bp fragments by Covaris S2 system, and libraries were generated using NEB DNA Library Preparation Kit following the manufacture’s protocol.
GEO Accession
GSM1639588