GSM4006686: Corpus luteum, P1; Bos taurus; RNA-Seq
For next generation sequencing (NGS), total RNA was isolated from all 8 samples using the mirVana RNA isolation kit (ThermoFisher). RNA quantity and quality were assessed using a BioRad Experion and all samples had an RNA quality index (RQI) > 9. Strand-specific sequencing was performed according to standard protocols at BGI. The library was constructed using 200 bp inserts of cDNA and library quality control was performed by qPCR, using an Agilent 2100 machine.
GEO Accession
GSM4006686