GSM3245015: 182G_HF_LF: GDM Low fat 3; Rattus norvegicus; RNA-Seq
Total RNA from the islets was extracted using RNAeasy Micro kit (Qiagen, Valencia CA, USA) as per manufacturer instructions. Subsequently, ribosomal RNA was removed from two micrograms total RNA by using Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat) (Illumina).  Total RNA and mRNA purity and integrity was assessed using Bioanalyzer 2000 Total RNA Pico and mRNA Pico, respectively (Agilent Technologies, Santa Clara CA, USA). The library was prepared according to NEBNext Ultra II Directional RNA Library Prep for Illumina (NEB, Ipswich, MA, USA).  Ribosomal RNA depleted RNA (10-100 ng) was fragmented and random primed, converted to cDNA and ligated to sequencing adaptors.  PCR enrichment of adaptor-ligated libraries was performed and libraries were analyzed and measured using Bioanalyzer 2000 2100 Expert DNA 1000 chip (Agilent Technologies, Santa Clara CA, USA).
GEO Accession
GSM3245015