GSM4630810: Kidney 11 IRI anti-C5 mAb; Mus musculus; RNA-Seq
Kidneys were perfused with saline and cut longitudinally upon harvest, then immediately submerged in RNAlater (QIAGEN). Samples were stored at 4°C for 24 hrs and then at −80°C. Before RNA preparation, kidneys were dissociated in RLT Plus (QIAGEN) with 1% β-mercaptoethanol in ceramic bead vials (Geneworks Pty Ltd) using a Cryolys cooling device and a Precellys 24 homogeniser (Bertin Technologies). RNA was then prepared using an RNeasy Plus Mini kit (QIAGEN). RNA integrity was confirmed on the 2100 Bioanalyser (Agilent Technologies). cDNA libraries were prepared for sequencing using standard strand specific kits. 100 bp single-end reads were generated using Illumina HiSeq 3000 using standard protocols at the Australian Genome Research Facility. Single-end
GEO Accession
GSM4630810