GSM2422362: C_TH_16; Mus musculus; RNA-Seq
The medial prefrontal cortex was dissected, minced, and resuspended in HBSS. 1% of the suspension was taken as the total homogenate, spun at 1000 x g and flash frozen in liquid nitrogren. RNA was harvested using Qiagen Rneasy Micro Kit (Cat No. 74004). Samples were Poly(A) selected using the Poly(A) Purist Kit (Thermo Fisher Scientific Inc.) and prepared using NEBNext® Ultra™ Directional RNA Library Preparation Kit (New England BioLabs, Ipswich, MA). Samples went through 15 cycles of PCR. Samples were sequenced on the NextSeq 500 (Illumina, San Diego, CA) using (75 bp; paired-end reads) to a minimum target depth of 20 million reads per sample.
GEO Accession
GSM2422362