GSM4683100: Naïve B cell; Mus musculus; RNA-Seq
Cells were partitioned into nanoliter-scale Gel Bead-In-EMulsions (GEMs) to achieve single cell resolution for a maximum of 10,000 individual cells per sample. Utilizing the Chromium Single Cell 5' Library & Gel Bead Kit (10x genomics, 1000006), poly-adenylated mRNA from an individual cell was tagged with a unique 16 base pair 10x barcode and 10 base pair Unique Molecular Identifier. The cDNA amplicon size for the library (~700 bp) was optimized using enzymatic fragmentation and size selection and the concentration of the 10x single cell library was determined (Agilent High Sensitivity DNA Kit (Agilent). The single cell 5' libraries were sequenced on the Illumina HiSeq-4000 sequencer (The NUSeq Core facility, Northwestern University) at 26 x 98 bp, paired end to target more than 50,000 reads per cell. We adopted Cell Ranger 3.0.2 (https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/what-is-cell-ranger) for raw sequencing process
GEO Accession
GSM4683100