NextSeq 500 paired end sequencing; FloChIP's decreasing cell number data obtained in multiplexing mode
FloChIP's decreasing cell number data obtained in multiplexing mode
FloChIP, custom (FloChIP technique is a novel microfluidic immunoprecipitation system, the details of which will be published in due course) PCR purification and ampure XP bead size selection Nextera with custom reagents and Tn5
Experimental Factor: immunoprecipitate
anti-H3k27ac (Abcam)
Experimental Factor: cell number
50 000