GSM3756993: NP_8; Bos taurus; RNA-Seq
Total RNA from each sample was isolated using the RNeasy Micro Kit (Qiagen, Germantown, MD, USA) according to the manufacture's cell protocol with the following adjustments for samples maintained in RNAlater; 250 µl of 100% ethanol was used instead of the recommended 350 µL of 70% (v/v) ethanol and isolated RNA was eluted in 12 µl RNase-free water instead of the recommended 22 µl RNase-free water. Barcoded fragment libraries were constructed using the Bovine Custom Any Deplete Ovation SoLo RNA-Seq System from NuGEN (San Carlos, CA, USA) following the manufacturer's protocol.