GSM4629796: Infection_1_ATAC_seq_midguts; Anopheles gambiae; ATAC-seq
We performed dissection of midguts on adults at 7 days post-infection and of salivary glands at 14 days post-blood meal. We performed the ATAC-seq protocol using fresh tissues from ~20 individual mosquitoes. Mosquito tissues were resuspended in lysis buffer to permeabilize membranes. The nuclei pellet was resuspended in the transposition reaction mix (25 μl of 2× TD Buffer, 1.25 μl of Tn5 Transposase and 23.75 μl of nuclease free water), and incubated for 30 min at 37°C. All samples were purified using the Qiagen MiniElute Kit. Library amplification was carried out with 2X KAPA HiFi mix and 1.25 μM of Nextera primers. The optimal cycle number was determined by qPCR. ATAC-seq libraries were sequenced at BGI (China) with an Illumina HiSeq4000 sequencer to obtain 25-37 M of 50 bp paired-end reads.
GEO Accession
GSM4629796