16S rRNA gene-based analysis of bacterial communities in soil of sample bag7
Metagenomic DNA was extracted from paddy soil without anaerobic incubation (biochar fertilizer), replicate G. Subsequently, the V3-V4 region of 16S rRNA genes was amplified. All samples were amplified in triplicate, purified and pooled in equal amounts. Sequences were determined using an Illumina MiSeq sequencer.