RNA-Seq of Mus musculus: splenic OT-I CD8 T cells educated 3d with antigen-pulsed mature dendritic cells
OT-I cells were selected by negative magnetic selection of CD8 cells from whole spleen of an OT-I mouse. They were then co-cultured with bone marrow dendritic cells, which had been pre-pulsed and matured with LPS ovalbumin and washed prior to addition of OT-I cells at a ~10:1 T:DC ratio. The non-adherent OT-I cells were isolated and treated with Qiagen RNeasy Plus mini kit to isolate mRNA, which was then submitted to EPFL Gene Expression Core Facility for library preparation and sequencing.