GSM2769733: Tbp ZT10; Mus musculus; ChIP-Seq
Livers were removed and flash frozen in liquid nitrogen. They were later homogenized in 1% PFA and the nuclei were isolated via sucrose density centrifugation. They were resuspended in SDS buffer and sonicated. The sonicated chromatin (major DNA fragment size ~150-400 bp) was aliquoted and used for ChIP. For Pol II ChIP, rabbit IgG was used as the control. For Nelf-A ChIP, goat IgG was used as the control. ChIP-seq SE50 libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM2769733