Bacterial 16S rRNA genes from Alyssum malacitanum rhizosphere in Sierra Bermeja S Spain, replicate 2
Total community DNA (TC-DNA) was extracted. purified TC-DNA was used as target to amplify the V3-V4 region of 16S rRNA genes with primers 338F/802R fused to Roche-454 A and B Titanium sequencing adapters and an eight-base barcode sequence in adaptor A. Library preparation and sequencing were done on a 454 Genome Sequencer FLX Titanium platform according to standard 454 protoccols(Roche 454 Life Science, Branford, CT) by BIOCANT, Genoinseq unit(Cantanhede, Portugal).