miRNA-Seq of wheat seeds
After total RNA wasextracted by TRIzol,the RNA molecules in a size range of 1830nt wereenrichedby polyacrylamide gel electrophoresis(PAGE). Then the 3adapters were added and the 36-44nt RNAs were enriched.The 5adapters were then ligated to the RNAs as well.The ligation products were reverse transcriptedby PCR amplification and the 140-160bp size PCR products were enriched to generate a cDNA library and sequenced using Illumina HiSeqTM2503 by Gene DenovoBiotechnologyCo. (Guangzhou, China).