GSM2497600: Csnk1e knockout mouse #3 : CB-4_L001; Mus musculus; RNA-Seq
Csnk1e KO and WT mice at about 50 days of age were live-decaptitated for brain dissections. As mentioned before, 2.5mm strialtal punches (left and right hemispheres) were pooled per mouse, and collected tissue was immediately places into RNAlater (QIAGEN) for 48 hours. After incubation, RNA was extracted via miRNeasy Mini Kit and quality was assessed by Bioanalyzer analysis. RNA that was deemed high quality was send to the University of Chicago Genomics Core Facility for cDNA library preparation. Libraries were prepared according to Illumina's detailed instructions accompanying the TruSeq® Stranded mRNA LT Kit  (oligo-dT; 50bp single-end reads; Part# RS-122-2101).  The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the HiSeq2500 following the manufacturer's protocols
GEO Accession
GSM2497600