GSM2497652: H3K27ac ChIP-seq TAC operation (Week 1); Mus musculus; ChIP-Seq
Cardiomyocytes (1,000,000 cells) were isolated from two mice 1 week after the TAC operation with Langendorff perfusion, were crosslinked with 1% formaldehyde at room temperature for 10 min and were quenched with 0.125 M glycine. After sonication, soluble chromatin was added with 4 μl recombinant histone 2B (New England Biolabs) and 0.5 μl of mouse RNA (Qiagen) and was incubated with the antibody-bead complex (5 μg of anti-H3K27ac antibody (Abcam, ab4729) and 20 μl of Dynabeads Protein G (Thermo Fisher Scientific, 10003D)) at 4 °C overnight. Immunoprecipitates were washed, reverse-crosslinked and incubated with proteinase K and RNase A. DNA was purified using a MinElute PCR Purification Kit (QIAGEN). Sequencing libraries were prepared using the TruSeq Kit (Illumina) for high-throughput sequencing on an Illumina HiSeq 2500 according to the manufacturer's protocol.
GEO Accession
GSM2497652