sRNA-seq of oryza sativa japonica group: seedling shoot total polysomes
After total polysome isolation by ultracentrifugation, total RNAs were extracted from the total polysomes of rice (Oryza sative japonica group, cv. Nipponbare) seedling shoots with TRI Reagent (Molecular Research Center, USA) and used for sRNA library construction according to the manufacturers instructions of NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB, USA).