16S rRNA gene sequences of thermophilic anaerobic digester: before increase of organic loading rate from 1.13 to 1.5 gVS/(L*day) (day 214 of experiment)
Two PCR reactions performed in triplicate. First step amplified the 16S rRNA genes and the second one attached Illumina-compatible barcodes and adapters. This was performed with primer sets 516F/915R for Archaea.