GSM4681864: IL22; Homo sapiens; RNA-Seq
Total RNA from differentiated Caco-2 BBe1 cells was prepared using RNeasy Mini Kit (Qiagen, Hilden, Germany) and contaminating DNA was removed using DNA-free DNA removal kit (ThermoFisher Scientific). mRNA libraries were prepared using the Illumina TruSeq protocol and sequenced using Illumina NextSeq 500 with read configuration as 76bp, single end. Each sample got approximately 42 million reads. Fastq files were checked for quality using fastqc (v0.11.2) and fastq_screen (v0.4.4). Fastq files were mapped to hg19 (UCSC version from igenomes) using STAR(v2.5.3a). Read counts were generated using featureCounts and the differential expression analysis was performed using edgeR. Genes with reads of log2 counts per million (CPM) > 0 and p-values < 0.05 were considered differentially expressed. Volcano plot was made using EnhancedVolcano.
GEO Accession
GSM4681864