GSM2586571: dCD_mESC_RNA-seq_rep3; Mus musculus; RNA-Seq
Cells were lysed in Trizol, RNA extracted, mRNAs purified with oligo(dT) beads and reverse transcribed with SuperScriptII. ChIP DNA, input DNA and cDNA were subject to end repair, A-tailing, adaptor ligation and cleavage with USER enzyme, followed by size selection of 250-500 bp and amplification with NEBNext sequencing primers
GEO Accession
GSM2586571