GSM4632675: hepatocyte_H3K27AC; Mus musculus; ChIP-Seq
Briefly, hepatocytes were extracted from the livers of 8-week old C57BL/6 mice using the protocol described in Foretz et al.,24. 1 million hepatocytes were cross-linked and sonicated for 6 cycles in order to shear chromatin in ~200-400bp length fragments. Mouse liver H3K27ac ChIP-seq was performed for this study using the ChIPmentation protocol described at http://www.medical-epigenomics.org/papers/schmidl2015/ ChIPmentation was performed according to protocol and post amplification libraries were cleaned using AMPure bead size selection yielding a library of DNA fragments between 200-500bp. Library was sequenced on an Illumina Next-Seq 500 to generate raw reads. 75bp single end reads
GEO Accession
GSM4632675