Samples collected from rhizosphere of G11 apple rootstock planted in fumigated soil: Sample "G11 fumigated- 16s, Rep 10"
"Bacterial DNA was amplified using a forward primer comprised of a 25-bp 454 A Adapter, an 8-bp barcode and modified universal bacterial primer16S-27F (5 ?-AGR GTT TGA TCM TGG CTC AG-3 ?) with a reverse primer comprised of a 25-bp 454 B Adapter and modified universal bacterial primer 16S-519R (5?- GTN TTA CNG CGG CKG CTG-3?). Fungal DNA was amplified using the 25-bp 454 A Adapter and an 8-bp barcode followed by the forward primer ITS1F (19) and the reverse primer consisting of the universal primer ITS4 (64) with the 25-bp 454 B Adapter.  "