SOLiD RNA-SEQ library for a single late-stage Poeciliopsis prolifica embryo
RNA was isolated from a single late-stage embryo from Poeciliopsis prolifica female #6 (from SRA run SRR1640209) using the Qiagen RNeasy kit. Tissue sample included offspring's belly sac, the embryonic portion of the organisms placenta. Ribosomal RNA was removed from RNA samples using Epicentre Ribo-Zero rRNA Removal kit. Sequencing libraries from resulting RNA products were constructed using standard SOLiD cDNA library construction protocols. All barcodes were removed post-sequencing using the program Cutadapt.