GSM1537955: Angptl2-Notch ChIPseq; Homo sapiens; ChIP-Seq
Lysates were clarified from sonicated nuclei and Notch antibody bound genomic DNA complexes Libraries were prepared as follows: DNA was end-repaired using End-It DNA Repair kit (Epicenter ER0720). The blunt, phosphorylated ends were treated with Klenow and dATP for ligation of Illumina's adapters. After adapter ligation DNA was PCR amplified with Illumina primers for 18 cycles and library fragments of ~250 bp were gel extracted and purified. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Illumina Hi-seq
GEO Accession
GSM1537955