GSM4630064: CtrlB-P22-R2-0430-d200-1; Homo sapiens; RNA-Seq
Organoids were snap frozen on dry ice at the indicated day of differentiation. Frozen samples were thawed on ice and RNA extracted using Qiagen Rneasy Mini Kit according to the manufacturer's manual. RNA quality was determined using Agilent Bioanalyzer, all samples had RIN >7. RNA was isolated from 3 frozen organoids per sample using QIAGEN RNeasy Kit according to manufacturer's manual. RNA quality was assessed using Bioanalyzer (Agilent Technologies). Samples with RIN >7 were subsequently used for library generation using TruSeq Library Prepartation Kit (Illumina Inc.).
GEO Accession
GSM4630064