GSM2424951: SSEA4+_SingleCells_SSCs_53; Homo sapiens; RNA-Seq
Isolated SSEA4+ or Kit+ germline stem cells were diluted into 15,000-20,000 cells/ml and loaded into 5-10 μm integrated fluidic circuits (IFCs) using Fluidigm C1 instrument. Single cells captured in IFCs were scored under microscope. Only cells with normal morphology were scored high for sequencing. Single cells in IFCs were lysed and total RNA was harvested for polyadenylation selection, reverse transcription and PCR amplification. Library constructions were performed according to Fluidigm Library preparation with Nextera XT protocol and sequenced on a 50-cycle single end run on an Illumina HiSeq 2500 instrument.
GEO Accession
GSM2424951