GSM3177397: Lazy_TG02; Danio rerio; RNA-Seq
Flash frozen skeletal muscle was lysed in Qiazol with the FastPrep®-24 tissue homogenizer (MP-Biomedials). Total RNA was extracted using the QIAcube plateform and mRNAeasy kit (Qiagen). Sequencing libraries were prepared from 250 ng RNA using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina) following the manufacturer's protocol, except for the PCR amplification step. The latter was run for 15 cycles with the KAPA HiFi HotStart ReadyMix (Kapa BioSystems). Libraries were quantified with Picogreen (Life Technologies). The size pattern was controlled with the DNA High Sensitivity Reagent kit on a LabChip GX (Perkin Elmer). Libraries were then pooled and the pool was clustered at a concentration of 9 pmol on 2*8 lanes of paired-end sequencing high output flow cell (Illumina). Sequencing was performed for 2 x 125 cycles on a HiSeq 2500 with v4 SBS chemistry strictly following Illumina's recommendations.
GEO Accession
GSM3177397