Expression analysis of RNA from temperature-pulsed cultures of Neurospora crassa
RNA was harvested from Neurospora crassa cultures grown in the light for 24 hours, dark for 24 hours, temperature pulse (30C) for one hour and then harvested. RNA was extracted using Qiagen's plant RNA extraction kit.  Library was prepared using the SOLiD Total RNA-seq kit. Two rounds of rRNA depletion was performed before the library prep using Invitrogen's (now Life Technologies) Ribominus kit. CH02 and CH08 are biological replicates.