16S rRNA sequences of Amazonian floodplain forest sediments
Genomic prokaryotic DNA of the V4 hypervariable region of the 16S rRNA gene was amplified using the barcoded primers 519f/915r (Klindworth et al., 2013; Stahl & Amman, 1991), specific for Archaea. Paired-end sequencing, with 2x250 bp reads, was performed in Illumina Hiseq 2500 platform, at Novogene Bioinformatics Technology (Beijing, China), using standard procedures.