Extraction blank of molecular-grade sterile water: Qiagen MagAttract PowerMicrobiome DNA/RNA KF Kit
Genomic DNA extraction was performed using the Qiagen MagAttract PowerMicrobiome DNA/RNA KF Kit, following the manufacturer's recommendations. Extracted DNA was subject to PCR amplification of the V4 hypervariable region of the 16S rRNA gene. Amplicons were subjected to paired-end sequencing (2x300 bp) on the Illumina MiSeq platform using the MiSeq Reagent Kit v3 chemistry.