GSM2424869: Rex1_16h_Rep1; Mus musculus; Bisulfite-Seq
Genomic DNA was isolated using Gentra Puregene Cell Kit (Qiagen) Genomic DNA was fragmented by sonication (Covaris) and adaptor ligated using Illumina supplied methylated adaptors and NEBnext library preparation kit. Subsequently DNA was bisulfite-treated using the Sigma Imprint kit according to the manufacturer’s instructions (one step protocol). Final library amplification (11 cycles) was done using KAPA Uracil+ (KAPA Biosystems), after which the libraries were purified using Ampure beads (x1).
GEO Accession
GSM2424869