GSM2424725: PCV2 uninfected Laiwu pig; Sus scrofa; miRNA-Seq
Total RNA was extracted through Trizol reagent Briefly, the 18–30-nt fraction of total RNA was excised and purified by 15% Tris-borate-EDTA (TBE) denaturing polyacrylamide gel electrophoresis (PAGE). Subsequently, 3′ and 5′ RNA adaptors were ligated to the purified fragments using T4 RNA ligase. The adaptor-ligated sRNAs were amplified by RT-PCR, and the cDNA was amplified further by running 15 PCR cycles. The PCR products (90-bp sRNAs+adaptors) were purified on 4% agarose gels and used for sequencing analysis on an Illumina 1G Genome Analyzer (Illumina) at the BGI.
GEO Accession
GSM2424725