cBKV27
Genetic material was centrifuged from 250 ml of water in bait or pond fish retailer tanks and DNA extracted with Qiagen DNeasy kits. Extractions were subjected to a two step library prep protocol. The first step utilized targe primers with spacer inserts of varying lengths (7-14 bp) and a portion of the Illumina MiSeq sequencing primer. The second step used Illumina Nextera indices, added to amplicons via PCR. These libraries were sequenced on an Illumina MiSeq with 2X300 chemistry.