GSM2152829: Dox_6hr_rep1; Mus musculus; RNA-Seq
RNA was isolated using an Rneasy mini kit according to the manufacturers instructions. libraries were prepared using 200ng of Total RNA and 14 cycles of amplification in the  Agilent Sure Select Automated Strand Specific RNA library prep for Illumina Multiplexed sequencing  (G9691-90030) on an Agilent Bravo-BenchCel-MiniHub  Automated Liquid Handling Platform. Libraries were sized on an Agilent Bioanalyzer (G2940CA) using a High Sensitivity DNA Kit (5067-4626) and quantified by qPCR using a Kapa Library Quantification Kit for Illumina sequencing platforms (KK4835). Pooled libraries were sequenced at 1 x 75bp on an Illumina NextSeq500 system (SY-415-1001).
GEO Accession
GSM2152829