GSM1639862: Syn_ESC_H3S10p_ChIPseq_2; Mus musculus; ChIP-Seq
For each sample, 1 x 10^6 cells were cross-linked with 1% PFA and cell nuclei were prepared using a swelling buffer (25 mM Hepes, pH 7.8, 1 mM MgCl2, 10 mM KCl, 0.1% NP-40, 1 mM DTT). For histone modifiacations,  chromatin was sheared to mononucleosomal fragments. For RNA-seq RNA was extracted and treated with RiboZero followed by DNaseI digestion. Libraries were prepared according to Illumina standard protocols with internal barcodes.
GEO Accession
GSM1639862