GSM2771251: T124R1; Plutella xylostella; miRNA-Seq
Total RNA was extracted from larvae using Triazol® following the manufacturer’s protocol (Life Technologies). RNA concentrations were measured using a spectrophotometer and integrity was ensured through analysis of RNA on a 1% (w/v) agarose gel. The small RNA libraries were generated from all samples with three biological replicates using the Illumina Truseq small RNA preparation kit at the Australian Genome Research Facility (AGRF-Melbourne, Australia). The purified cDNA libraries were sequenced on Illumina HiSeq and raw sequencing reads (50 nt) were obtained using Illumina’s Sequencing Control Studio software.
GEO Accession
GSM2771251