GSM2425116: ChIPSeq_WT_p16_STAT5A_lactation day 1; Mus musculus; ChIP-Seq
Frozen-stored mammary tissues from p14, p16, and L1 were broken into powder with mortar and pestle. Nuclei were extracted in Farnham lysis buffer (5mM PIPES pH8.0, 85mM KCl, 0.5% NP-40 and protease inhibitors). Antibodies against H3K27ac (Abcam, Ab4729), STAT5A (Santa Cruz, sc-1081), CTCF (Millipore, 07-729) were used for ChIP. Libraries were prepared for sequencing using standard Illumina protocols.
GEO Accession
GSM2425116