GSM2157041: 1266EV2S (Mex67 IP 42°C); Saccharomyces cerevisiae; RNA-Seq
Eluates were purified via trizol-chloroform (Ambion® RNA by Life technologies™) extraction. Removal of contaminating DNA was performed using the TURBO DNA-free kit (Ambion® RNA by Life technologies™). 500 ng of total RNA were used as start material for library preparation. Quality and quantity of RIP RNA was determined by using the Fragment Analyzer (Advanced Analytical). We started directly with the fragmentation of the RIP samples (200 bp) and cDNA synthesis. mRNA enrichment or depletion of rRNA was avoided. The samples were prepared with the "TruSeq RNA Sample Prep Kit v2" protocol from Illumina. Libraries were validated by using the Fragment Analyzer for sizing and the QuantiFlur Kit from Promega for library quantitation.
GEO Accession
GSM2157041