GSM2842967: human_brain_MNase-seq; Homo sapiens; MNase-Seq
Pellets were washed with MNase digestion buffer (10mM Tris pH7.4, 15mM NaCl, 10mM KCl, 1.5mM CaCl2), after which were collected by 350g centrifugation for 5 min at 4°C. Pellets were further suspended with digestion buffer containing MNase (NEB, M0247S) and incubated at 37°C, then stopped by 2× stopping buffer (0.6% SDS, 40mM EDTA, 40mM EGTA). E7370 kit was used for library construction. And the samples were indexed using Illumina Truseq indexes. The cycle number of the amplification was 4 or 5.
GEO Accession
GSM2842967