RNA-Seq of Rhinella arenarum: larvae: whole organism: MAZ 24h treated
Total RNA was extracted using Thermo Scientific GeneJET RNA Purification Kit according to the manufacturers instructions. The RNA quantity and quality were evaluated using Agilent RNA 6000 pico kit in a 2100 Bioanlayzer (Agilent Technologies) (Perfiles en material complementario?). All samples presented excellent condition with an RNA Integrity Number above 8 (RIN>8). The cDNA library for transcriptome analysis was prepared using TruSeq RNA v2 kit according to the manufacturers protocol (Illumina, San Diego, CA, USA). Briefly, mRNA enrichment was carried out using oligo dT magnetic beads, chemical fragmentation of the purified mRNA into small pieces, double-stranded cDNA synthesis, end repair and adenylation processes. Finally, adaptor ligation and enrichment by PCR to create the final library with an average size of 260bp were done.