454 RNA-SEQ sequencing library for whole ovary,placenta, and associated embryo sample from Poeciliopsis prolifica
RNA was isolated from tissue sample contain entire ovary, including all placental tissues, embryos spanning numerous developmental stages and fertilized eggs using the Qiagen RNeasy kit. RNA was then subjected to Poly-A selection and sequencing library was constructed from the resulting Poly-A selected RNA using standard 454 cDNA library construction protocols.