GSM2231447: E13.5 Ngn3-high #2; Mus musculus; RNA-Seq
Total RNA were isolated from about 4-6 X 10e4 sorted cells by RNeasy Micro Kit (Qiagen, 74004). mRNA was purified from ~200 ng of total RNA by “NEBNext Poly(A) mRNA Magnetic Isolation Module” (NEB #E7490). Libraries were prepared using” NEBNext Ultr Directional RNA Library Prep Kit for Illumina” (NEB #E7420) following the manufacturer's instructions.
GEO Accession
GSM2231447