Ion Torrent Proton sequencing
The RNA-Sequencing was performed by sequencing of short reads of RNA on the Ion ProtonTM (Thermo Scientific, Wilmington, USA) next-generation sequencer
After RNA isolation, single-stranded complementary DNA was synthesized using Reverse Transcription System kit (Maxima H Minus Double-Stranded cDNA Synthesis Kit K2561, Thermo Scientific, Wilmington, USA).