GSM4006838: HUVEC_H_T3d_HiC; Homo sapiens; OTHER
The HiC libraries generation followed instruction of Arima HiC kit. Briefly, ~5-10 million cells were crosslinked with 1% formaldehyde at room temperature (RT) for 10 min. Approximately 1 million crosslinked cells (2-5 ug of DNA) were lysed with Arima's Lysis Buffer and digested with Arima restriction enzyme cocktail. The 5'-overhangs were then filled in and labeled with biotinylated nucleotides. Then, spatially proximal digested ends of DNA were ligated. The ligated DNA was purified and sheared by Covaris ultra-sonicator to obtain average DNA size of 400 bp. Then, the biotinylated fragments were enriched with Arima Enrichment Beads. Afterwards, KAPA Hyper Prep reagents were used in a custom end-repair, dA-tailing and adaptor ligation protocol. The bead-bound Arima HiC libraries were PCR amplified, producing the sequence-ready Arima HiC libraries.
GEO Accession
GSM4006838