GSM2425099: ESC_ATAC_seq_rep2; Homo sapiens; ATAC-seq
Collected cells were lysed in cold lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2 and 0.1% IGEPAL CA-630) and the nuclei were pelleted and resuspended in Transposase buffer. The Tn5 enzyme was made in house and the transposition reaction was carried out for 30 minutes at 37°C. Following purification, the Nextera libraries were amplified for 12 cycles using the NEBnext PCR master mix and purified using the Qiagen PCR cleanup kit. All libraries were sequenced on a 125-cycle paired-end run on an Illumina HiSeq 2500 instrument.
GEO Accession
GSM2425099