Whole-genome bisulfite sequencing of cashmere goat: fetal E65 skin 2
After the DNA sample was qualified, a certain proportion of positive control (lambda) was added. First, Covaris S220 was used to randomly interrupt the genomic DNA to 200-300bp.Repair the ends of broken DNA fragments, add A tail, and connect the sequencing connector.Then, Bisulfite treatment (EZ DNA methylation GOLD,Zymo Research) was performed, and the unmethylated cytosine was transformed into uracil (that is, C was converted to U, and then T after PCR amplification), while the methylated cytosine C remained unchanged, then PCR amplification was conducted to obtain the final sequencing library.After the constructed library was qualified by Agilent 2100 Bioanalyzer, Illumina HiSeqTM 2500 sequencer was used for sequencing. The sequencing read length was 125bp of Paired-End sequencing.