RNA-Seq of Rat supraoptic nucleus (SON) under normosmotic and salt-loading condition
Amplified cDNA libraries were prepared from the isolated RNA samples shown in Table 1 and sequenced by the NIH Intramural Sequencing Center (NISC), Bethesda, MD under the direction of Baishali Maskeri and Alice Young.  Briefly, mRNA libraries were constructed from 50-750 ng mRNA using the Illumina TruSeq RNA Sample Prep Kits, version 2.  The resulting cDNA was fragmented using a Covaris E210.  Library amplification was performed using 12-15 cycles to minimize the risk of over-amplification.  Unique barcode adapters were applied to each library.  Equal volumes of individual libraries were pooled and run on a MiSeq.  The libraries were then repooled based on the MiSeq demultiplexing results and sequenced on a HiSeq 2000 with ver 3 flow cells and sequencing reagents.  At least 4x40 million 100-base read pairs were generated for each individual library.  Data was processed using RTA 1.13.48 and CASAVA 1.8.2, providing for four sets of paired .fastq files per library.