GSM4194551: BKO_input; Mus musculus; RIP-Seq
Total RNAs were extracted using TriPure Isolation Reagent (Roche, Mannheim, Germany) from iBAT of Mettl3flox/flox and BKO mice . Poly(A)+ RNA was purified using Dynabeads™ mRNA Purification Kit (Invitrogen) following the manufacturer's instructions. Chemically fragmented poly(A)+ RNA was incubated with m6A antibody (Synaptic System, 202003) for immunoprecipitation following the standard protocol of Magna MeRIPTM m6A Kit (MERK, 17-10499). Enrichment of m6A mRNA was then analyzed by high-throughput sequencing using Illumina Hiseq X platform.
GEO Accession
GSM4194551