GSM2843889: RHT2476; Mus musculus; RNA-Seq
Adipose tissues were lysed in TRIzol (Thermo Fisher Scientific, Waltham, MA, USA) and RNA was isolated followed by a Qiagen RNeasy Micro clean-up procedure (Qiagen, Hilden, Germany) according to the manufacturer's protocol. cDNA libraries were prepared using 2ng of total RNA and 1ul of a 1:50,000 dilution of ERCC RNA Spike in Controls (Ambion® Thermo Fisher Scientific, Waltham, MA, USA) using SMARTSeq v2 protocol  except for the following modifications: 1. Use of 20µM TSO, 2. Use of 250pg of cDNA with 1/5 reaction of Illumina Nextera XT kit (Illumina, San Diego, CA, USA).
GEO Accession
GSM2843889