GSM3692304: No Injury; Mus musculus; RNA-Seq
B6.Cg-Pax7tm1(cre/ERT2)Gaka/J (Pax7CreERT2) was crossed with the B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J(Ai9) to yield the Pax7Tdt(Pax7CreERT2:R26Rtdtomato) mice, which were both obtained from Jackson Labratory. Kdrtm2.1Jrt/J(Flk1GFP) were obtained from Jannet Rossant(Ema et al. 2006). Cre recombination was induced using tamoxifen (Sigma-Aldrich, T5648) dosed as 75mg/kg body weight x 3 times over one week, followed by a 5 day chase period, in 8 week old Pax7CreERT2/ CreERT2:R26Rtdtomato/tdtomato Flk1GFP/+. To induce injury and regeneration, TA muscle were injected with 20 ul of 3 mM cardiotoxin (CTX; Sigma-Aldrich, 217503). Both TA muscles from each mice were dissected and a single cell was obtained following enzymatic digestion as previously described (Verma M. 2018. Cell Stem Cell). We loaded ~5,000 cells into 1 channel of the Chromium system for each of these samples and prepared libraries according to the manufacturer's protocol using version 2.0 chemistry (10x Genomics). Following capture and lysis, we synthesized cDNA and amplified for 12 cycles as per the manufacturer's protocol (10X Genomics). The amplified cDNA was used to construct Illumina sequencing libraries that were each sequenced on one lane of an Illumina HiSeq 2500 machine
GEO Accession
GSM3692304