GSM1419279: CD4_H3K4me3_JMJD3-KO_Rep2_GA2537; Mus musculus; ChIP-Seq
Mouse splenocytes were isolated using a negative selection kit (StemCell) and stained for CD4, CD25, CD44, and CD62L. Naïve CD4+ (CD4+ CD25- CD44low CD62L+) cells were sorted by BD FACSAria II to >95% purity. Library construction was as described in Barski et al. Cell 129(4): 823-837 (2007).
GEO Accession
GSM1419279