Illumina HiSeq 2000 paired end sequencing
DNA was extracted using Qiagen Midi extraction kits (Qiagen, UK), and 500ng of DNA from each sample was sheared and prepared for sequencing using Illumina�s HiSeq2000 platform. Sequencing libraries were prepared in pools (read lengths of 93 to 101 bp; insert size of 400 bp) with the Kappa Illumina paired-end library preparation protocol.