GSM2844574: INS-1 S487D 3; Rattus norvegicus; RNA-Seq
RNA was harvested using Trizol reagent.The purity was assessed using the ND-1000 Nanodrop. Each RNA sample had an A260:A280 ratio above 1.8 and A260:A230 ratio above 2.0. RNA integrity was evaluated using the Agilent 2200 TapeStation (Agilent Technologies, USA) and each sample had the RINe above 7.0. mRNAs were isolated from Total RNA and fragmented to approximately 200bp. Subsequently, the collected mRNAs were subjected to first strand and second strand cDNA synthesis following by adaptor ligation and enrichment with a low-cycle according to instructions of TruSeq® RNA LT/HT Sample Prep Kit (Illumina, USA). RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM2844574