RNA-seq of HEK293FT cells 12h after let-7a transfection
A total amount of 5 μg RNA per sample was used as input material for the RNA sample preparations. Briefly, Poly(A)+ RNA selected on oligo-dT25 DynaBeads and fragmented. After 3' and 5' adaptor ligation, cDNA was synthesized using RT primer and SuperScritp III Reverse Transcriptase. Prepared cDNAs were amplified by PCR with Phusion High-Fidelity DNA polymerase, purified, and sequenced on Illumina Hiseq platform and single-end reads were generated.