GSM1536403: SCC_Acla_Low_spike-in_(20140617_6); Saccharomyces cerevisiae; Mus musculus; OTHER
Aclarubicin-treated mouse SCC cells and untreated yeast cells were cross-linked at room temperature with 2% formaldehyde and incubated with 125 mM glycine. The cells were then incubated with lysis butter and nuclear break buffer for 45min at 37 degrees C for nuclei isolation. The nuclei were quickly digested by proteinase K (100 microgram/ml) at 37 degrees C for 4 min, and then washed twice with NEB buffer 2 and once with quick blunting buffer followed by end blunting using quick blunting kit (New Englan Biolabs). The nuclei where then washed and subject to ligation with biotin labeled proximal linker at 16 degrees C for 18 hours. After ligation, genomic DNA was isolated and digested with HaeIII, followed by streptavidin beads capture, ligation of distal linker, digestion of I-Scel, PCR amplification, and XhoI digestion before Illumina sequencing.
GEO Accession
GSM1536403