GSM2425413: PBMYC1; Mus musculus; RNA-Seq
Mouse dorsal-lateral prostates were freshly dissected, dissociated using Collagenase into single-cell suspension, and Gli1-GFP positive cells were isolated using fluorescence activated cell sorting (FACS). RNA was extracted using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. SMART-seq V4 (Clonetech) ultra low input RNA kit was used for library preparation. 10 ng of amplified cDNA was used to prepare Illumina HiSeq libraries with the Kapa DNA library preparation chemistry (Kapa Biosystems) using 8 cycles of PCR.
GEO Accession
GSM2425413