unspecified sequencing; qiime_experiment_2105:1258247:905
study of eukaryotic diversity in Central Park, NYC
This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to ?1,500 samples per lane, and both PCR primers contain sequencer adapter regions.
experiment_title
centralpark18S
target_subfragment
V9
sample_center
CCME
sequencing_meth
sequencing by synthesis
run_date
4/8/14
experiment_center
CCME
run_center
CCME
num_sequences
106826
platform
Illumina
library_construction_protocol
This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to ?1,500 samples per lane, and both PCR primers contain sequencer adapter regions.
experiment_design_description
study of eukaryotic diversity in Central Park, NYC
row_number
905
target_gene
18S rRNA
study_center
CCME