GSM4681682: TYR_BR3; Salmonella enterica subsp. enterica serovar Enteritidis; RNA-Seq
Each bacterial culture (5 ml) was centrifuged at 5,000 rpm at 4°C for 10 min. The bacterial pellets were resuspended in RNA later (Invitrogen, USA) and stored at -80°C until further processing. The samples containing RNA later were thawed and resuspended in 750 µl of phosphate buffered saline (PBS) to decrease the viscosity and to facilitate the re-centrifugation of the bacterial cells. The supernatant was discarded, and the bacterial pellets were dissolved in 1.5 ml of TRIzol (Invitrogen, USA) and processed following the total-RNA extraction protocol as recommended by the manufacturer. The isolated mRNA was purified, fragmented and primed for cDNA library synthesis using TruSeq RNA sample prep kit (Illumina, USA) following manufacturers recommendations.
GEO Accession
GSM4681682