WGS for Phalangium opilio, 4th generation inbred male; adult
Single Molecule Real Time (SMRT) bell libraries were prepared according to the "20 kb Template Preparation Using BluePippin Size-Selection System" as recommended by Pacific Biosciences (https://www.pacb.com/wp-content/uploads/2015/09/Procedure-Checklist-20-kb-Template-Preparation-Using-BluePippin-Size-Selection.pdf, Palo Alto, U.S.A). After damage-repair the libraries were size-selected on a BluePippin system (0.75% (w/v) agarose gel cassette, dye-free, S1 marker, high pass 20kb protocol) to remove library fragments smaller than 10kb. Then libraries were recovered by PB AMPure beads, quantified by the high sensitivity fluorometric assay (Qubit, Thermo Fisher Scientific, Waltham, U.S.A.) and quality assessed using the genomic assay on the TapeStation (Agilent, Waldbronn, Germany). The prepared library was run on the PacBio Sequel platform (Pacific Biosciences) using version 2.1 chemistry. The Single Molecule Real-Time (SMRT) Cells was sequenced on (16) SMRT cells with 360min movie lengths.