Simultaneous Emergence of Multidrug-Resistant Candida auris on 3 Continents Confirmed by Whole-Genome Sequencing and Epidemiological Analyses
Genomic DNAs were sheared using focused ultrasonication (Covaris, Inc.,         Woburn, MA) and size selected using Ampure magnetic beads (Beckman Coulter, Indianapolis,         IN). Indexed adapters were ligated to DNA fragments and libraries were purified and PCR         amplified. Finished libraries were analyzed for size distribution using a Fragment Analyzer         capillary electrophoresis system (Advanced Analytical, Ankeny, IA) and concentrations         measured by Picogreen assay (Molecular Probes Eugene, OR). After denaturing, the paired-end         libraries were diluted and loaded onto Illumina (San Diego, CA) flowcells for cluster         formation. Clusters were then sequenced on an Illumina HiSeq 2500 using TruSeq SBS chemistry         producing paired-end reads of 100 basepairs. Post-run processing and de-multiplexing was         performed using CASAVA.