GSM1537609: PPARy-siRNA3; Homo sapiens; RNA-Seq
RNA was isolated and purified using the protocol of Nucleospin RNA II-kit and NucleoSpin® RNA Clean up-kit (Macherey-Nagel, BioTop Oy, Turku, Finland). RNA samples were prepared for Illumina RNA sequencing using instructions provided by New England BioLabs® Inc (NEB BioLabs, Ipswitch, MA, USA). Total RNA of 1-5μg was used as starting material when isolating poly(A)+ RNA transcripts from total RNA for RNA library preparation and sequencing using instructions provided by New England BioLabs® Inc.
GEO Accession
GSM1537609