Clinical Isolate of Pseudomonas aeruginosa WGS
The genomic DNA from 1e6 - 2e6 Pseudomonas aeruginosa cells was extracted with the Qiagen Blood and Tissue kit. 24 nanoliters of Nextera (Tn5) enzymes were used to process 50 picograms of genomic DNA into whole genome shotgun libraries in our microfluidic library construction device. The tagmented libraries were then collected and barcoded through enrichment PCR in a tube.