GSM1638844: Myo1-GFP-HP-Replica2; Schizosaccharomyces pombe; ChIP-Seq
Chromatin was extracted by glass bead cell lysis. Sonicated cell lysates containing chromatin were clarified and used for the immunoprecipitation step. 40µl of GFP-Trap agarose beads (Chromotek) were incubated with 300µl of chromatin extract, overnight at 4˚C. Cross-link was reversed and DNA purified using QIAquick PCR purification column. ChIP DNA from each sample was used undiluted for library construction. Library preparation has been performed with NEBNext® Ultra™ DNA Library Prep Kit (New England Biolabs), with the following modifications: indexing was performed using the TruSeq system and for library amplification KAPA HiFi Polymerase (KAPA Biosystem) was used. Quality control was carried out by Bioanlyzer 2100 system (Agilent) and qPCR quantification by KAPA Library Quantification Kit (KAPA Biosystem). 50bp or 100bp single-end sequencing was performed on pooled libraries using respectively a MiSeq or HiSeq 2500 (Illumina) (see table).
GEO Accession
GSM1638844