GSM4568902: ATAC-seq_gata2b het 1; Danio rerio; ATAC-seq
After euthanasia, the adult zebrafish were subjected to cardiac bleeding to remove most mature erythrocytes from the marrow. The zebrafish were then dissected at room temperature and the dissected marrow was placed into 500ul of 0.9x PBS with 2% FBS and 1 USP units/mL heparin, placed on ice. The marrow samples were then mechanically dissociated by repeated pipetting into single cell suspension and passed through a 40-μm nylon mesh. All 8 samples were washed by pelleting the cells at 350 x g for 5 min at 4C and resuspended in 300ul of 1x DPBS with 0.04% BSA (Gemini Bio-Products). Sci-ATAC samples were fixed and protocol for sample preparation was followed. 10x Genomics samples were subjected to nuclear isolation with lysis of 7 minutes, followed by 10x Genomics single-cell ATAC sequencing protocol as per manufacterer's recommendations. Single-cell ATAC sequencing, sci-ATAC (LaFave et al. Cancer Cell 2020, in press) and 10x genomics. We followed the 10x genomics sample preparation protocol and the 10x genomics single cell ATAC sequencing V1 library preparation
GEO Accession
GSM4568902