RNA-Seq of Chlorella sorokiniana ACETATE ADDED TO MEDIUM
RNA extraction was performed by modifying TRIzol SIGMA-ALDRICH protocol. Cells were disrupt by glass beads (Micro-organism lysing VKmix, Bertin Technologies) and the TRIzol reagent step was repeated two times. To clean samples and treat them with DNase SIGMA Spectrum Plant Total RNA kit was used. Each sample was cultivated and extracted in three indipendent biological replicates. RNA quality and quantity were determined using a Nanodrop 2000 spectrophotometer (Thermo Scientific, Wilmington, DE) and a Bioanalyzer Chip RNA 7500 series II (Agilent, Santa Clara, CA). Directional RNA-seq library preparation was performed starting from 1 ug total RNA using the TruSeq RNA Sample Prep Kit v2 (Illumina Inc., San Diego, CA, USA) after capturing poly-adenylated transcripts.. Library quality was assessed with a High Sensitivity DNA Kit on a 2200 Tape Staion (Agilent, Wokingham, UK). Libraries were sequenced with an Illumina HiSeq 1000 sequencer