GSM3701150: WT mouse 3; Mus musculus; RNA-Seq
Total RNA was extracted using Quiagen micro RNA purification kit The RNA was pre-amplified and converted to cDNA using Nugen Ovation RNA-seq System V2. Amplified cDNA was sonicated 10 cycles on a BioRupture (30sec on, 30sec off, High setting) and RNA-seq libraries were generated. Quantity and quality of the libraries was performed using Qubit HS assay, KAPA Biosystems library quantification kit for Illumina platforms and Agilent High Sensitivity DNA Assay. Next generation sequencing of the libraries was performed on a Nextseq 500 (Illumina). Reads where mapped using RNA STAR16 (Galaxy Version 2.4.0d-2) mapped reads where counted using htseq-count17 (Galaxy Version 0.6.1galaxy1). Differentially expressed genes were identified using DESeq218 (Galaxy Version 2.1.8.3).
GEO Accession
GSM3701150