RNA-Seq of Meriones unguiculatus: adult male liver
Total RNA was extracted from 50  100 mg liver tissue using TRIzol Reagent (ThermoFisher #15596026) and total RNA quality was assessed on non-denaturing agarose gel (1% agarose, 1x TBE buffer). mRNA was isolated from purified RNA samples using the polyA Spin mRNA Isolation Kit (NEB #S1560) and treated with the TURBO DNA-free kit (Invitrogen #AM1907) to further remove any contaminating DNA. cDNA libraries were prepared following Nanopore protocol Direct cDNA Sequencing (SQK-DCS108) and sequenced using flow cell FLO-MIN106 on a MinION.