GSM1419597: WT_35S_MethylC-Seq; Arabidopsis thaliana; Bisulfite-Seq
Genomic DNA was extracted from 14-day-old seedlings using the Plant DNeasy Maxi Kit from Qiagen and sent to BGI (Shenzhen, China) for bisulfite treatment, library preparation, and sequencing. Bisulfite sequencing libraries were prepared using standard Illumina protocols. The brief pipeline of BGI is as follows: 1. Fragment genome DNA to 100-300bp by Sonication. 2. DNA-end repair, 3’-dA overhang and ligation of methylated sequencing adaptors. 3. Bisulfite treatment by ZYMO EZ DNA Methylation-Gold kit. 4. Desalting, size selection, PCR amplification and size selection again. 5. Qualified library for sequencing.
GEO Accession
GSM1419597