GSM4681515: ATAC-P17-WT-3; Mus musculus; ATAC-seq
Flash-frozen quadriceps muscle samples from post-natal day 17 mice were added 1 mL of homogenization buffer (5mM CaCl2, 3mM Mg Acetate, 10mM Tris pH 7.8, 320mM Sucrose, 200uM EDTA, 0.1% NP-40, 0.05% BME, with Complete protease inhibitor cocktail (Sigma, 11697498001)) and disrupted with beads in Precellys Evolution (3x20s at 6800rpm). Lysate was sequentially filtered through 70um and 40um cell strainers, laid on top of sucrose buffer (1M sucrose, 3mM Mg acetate, 10mM Tris pH 7.8) and centrifuged at 1000g for 10min. Nuclei in the pellet were permeabilized in 0.5mL of 0.3% Triton in PBS for 30 minutes and washed twice with resuspension buffer (10mM NaCl, 3mM Mg Acetate, 10mM Tris pH 7.8). Omni ATAC-seq PMID: 28846090 and Illumina Nextera DNA Library Prep Kit (#15028212)
GEO Accession
GSM4681515