GSM1636934: MCF7_Pol2_N20_ChIP_B; Homo sapiens; ChIP-Seq
Cells were cross-linked with formaldehyde. Chromatin was sheared using Covaris S220 sonicator and immunoprecipitated on magnetic beads containing the antibody. Crosslinks were reversed by heating at 56'C overnight. The precipitated DNA was purified from protein by proteinase K and phenol/chloroform treatments, and used for preparation of a library using Illumina ChIP-seq library preparation kit All material was extracted from untreated MCF-7 cells Illumina TruSeq small RNA or ChIP-seq library preparation kits were used to prepare libraries
GEO Accession
GSM1636934