GSM2585967: im-nc_Col_rep2; Arabidopsis thaliana; ncRNA-Seq
Seedling roots were flash frozen immediately after harvesting and subjected to total RNA extraction using miRNeasy Plant Mini kit (QIAGEN). The total RNA was submitted to Texas A&M AgriLife Genomics & Bioinformatics Service for RNA-seq analysis (paired-end, 125-bp). After validating the integrity and purity of total RNA using Nanodrop, sequencing libraries were prepared using TruSeq Stranded Total RNA with Ribo-Zero Plant kit.  To enrich intermediate non-coding RNA (im-ncRNA), fragmentation step was omitted, and library cDNA with 100-500nt were purified using Pippin (Sage Science) prior to sequencing by Illumina HiSeq (125 nt, paired-end).
GEO Accession
GSM2585967