The genome sequence of the popular hexose transport deficient Saccharomyces cerevisiae strain EBY.VW4000
Genome sequencing was performed using Illumina HiSeq2000 (Illumina, CA, USA) at Baseclear (Leiden, The Netherlands). Genomic DNA of the strain EBY.VW4000 was isolated using the Qiagen 100/G kit (Qiagen, Hilden, Germany) following manufacturer’s recommendations and it was used to obtain a 50-cycle mate pair library with an 8 kb insert size. For a second library, fragments of approximately 180-200 bp were sequenced paired-end with a read length of 100 bp. The overlapping read pairs were merged into single longer (pseudo)reads. This generated, for both libraries, more than 16 million reads with a total sequence of 2200 million base pair resulting in approximately 170-fold coverage. Genome assembly was performed on the (pseudo)reads library using the GSAssembler 2.6, also known as Newbler (454 Life Sciences/Roche), using default settings