GSM4194669: U2OS H3K4me1-anaphase/telophase-ChIP-seq-r1; Homo sapiens; ChIP-Seq
Cells were cross-linked with 1% formaldehyde at room temperature for 10 min and neutralized with 0.125 M glycine for 5 min . After sonication, 25 ug of chromatin was incubated with 5 μg of anti-H3K4me3, anti-H3K4me1, anti-H3K27ac, or anti-CTCF antibodies at 4°C overnight. For spike-in normalization, 50 ng of Spike-in chromatin (53083, Active Motif) with 2 μg Spike-in antibody were added. Immunoprecipitated complexes were collected using Dynabeads M280 sheep-anti-rabbit IgG (Invitrogen). Subsequently, immuno-complexes were washed, decorsslinked and DNA was purified by QIAquick Spin columns (Qiagen). Cells (5 × 106) were cross-linked with 1% formaldehyde for 10 min, and ChIP-seq was performed as previously described (Toyama et al. 2018). Spike-in was carried out according to vendor protocols (Active Motif). Briefly,  DNA libraries were generated using the Kapa Hyper Prep Kit for Illumina Platforms (Kapa biosystems). Libraries were sequenced in a NextSeq 500 system (Illumina). ChIP-seq libraries were generated using the Kapa Hyper Prep Kit for Illumina Platforms (Kapa biosystems).
GEO Accession
GSM4194669