Illumina HiSeq 2500 paired end sequencing; Liver transcriptome profile associated with feed efficiency in Nellore Cattle (Bos indicus)
Liver transcriptome profile associated with feed efficiency in Nellore Cattle (Bos indicus)
liver samples were collected by biopsy and, immediately frozen in liquid nitrogen  and kept in -80°C freezer until RNA extraction RNeasy mini kit (QIAGEN, Crawley, West Sussex, UK) was used in accordance with guidelines supplied by manufacturer to extract total RNA from around 30 mg liver biopsy samples Preparation of libraries were carried out using TruSeq™ RNA Sample Prep Kit in accordance with TruSeq® RNA Sample Preparation v2 Guide (Illumina, USA, 2012, Part # 15026495 Rev. D)
Experimental Factor: diet
high feed efficiency
LIBRARY_STRAND
not applicable