gut microbita (16S V3-V4) seq of infant B20
Stool microbial genomic DNA was extracted from cattle fecal sample, V3-V4 region of bacteria 16S rRNA was amplified from the isolated microbial genomic DNA. The nucleotide sequence of the primer was 341F: 5'-CCT AYG GGR BGC ASA CG-3', 806R: 5'-GGA CTA CNN GGG TAT CTA AT-3'. PCR fragments with desired size (approximately 465bp) was purified, DNA quality and concentration was checked. Each sample had 3 replications. The purified fragments of each sample were normalized and pooled. Pyrosequencing of the samples was sequenced using Ion Torrent S5 XL.