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PCR of 16S using general bacterial primers 515F and 806R from Caporaso et al. (2010), PNAS.   Samples were amplified in triplicate using the same conditions and chemistry described in the above paper. Samples were gel cut and purified with the Qiagen Qiaquick gel extraction kit and then quantified with the Qubit. Pooled samples were sequenced on an Illumina MiSeq using 151-bp paired-end reads using V2 chemistry,