Small RNA-seq of HEK293T: Phosphatase Treatment, Replicate 2
Small RNAs (<70 nt) were isolated by PAGE purification. The small RNAs were treated with or without RNA 5? polyphosphatase. RNA was then recovered via ammonium acetate precipitation in 75% EtOH. Small RNA libraries were prepared for Illumina small RNA-seq using the multiplex small RNA library preparation set (New England Biolabs).