MiSeq of Cladocopium ITS2 type C15b.2:Goniastrea retiformis coral host
A 350bp segment of the internal transcribed spacer region 2 (ITS2) rDNA region was amplified using Symbiodiniaceae specific primers, ITS-Dino-forward and its2rev2-reverse. Each primer also contained a universal linker, for downstream incorporation of Illumina adapters and barcodes. Following barcoding PCR, samples were pooled and sequenced on an Illumina MiSeq (250bp paired-end Reagent Nano Kit v2).