the USP7 knockdown effect on transcriptome of LNCaP prostate cancer cells
LNCaP prostate cancer cells were exposed to vehicle for 16 h and subjected to RAN extraction.Total RNA was isolated using Trizol (Invitrogen) and Nucleospin RNA II (Macherey-Nagel). One microgram of mRNA was used for library preparation using Illumina TruSeq RNA sample Preparation v2 kit (TruSeq).