Impact of ERK mediated Dicer phosphorylation on Small RNAs
TotalRNA was extracted using the mirVana kit (Qiagen Kit), and quality and concentration of RNA were determined by an Agilent 2100 Bioanalyzer. Small RNA libraries were prepared with a small RNA version 1.5 sample preparation kit (Illumina, San Diego, CA). 5' Mono phosphate ends were captured for preparing the libraries.  For each experiment, 2000 animals of the identified genotype were synchronized at mid-larval stage 4 of development and allowed to develop for indicated time points. Total RNA was then extracted from each of these samples. All libraries were sequenced to a 54-nucleotide (nt) read length on individual lanes of a Genome Analyzer  (Illumina, San Diego, CA).