GSM2770952: R1-0006 (FSIS-FY14-9) control [F9C29_C]; Salmonella enterica subsp. enterica serovar Heidelberg; RNA-Seq
RNA was extracted using Trizol (Ambion, Foster City, CA) with an additional phenol:chloroform extraction  in triplicate for each isolate and condition. RiboZero (Illumina, San Diego, CA) was used to remove rRNA; TURBO DNase (Thermo Fisher, Waltham, MA) was used to remove DNA. DNA removal was assessed using qRT-PCR for rpoD and gyrB (Table 3.2) (25, 34, 61, 80). Primers and probes were purchased from Integrated DNA Technologies (IDT, Coralville, IA); qRT-PCR was performed using IDT’s 2x master mix. If negative control samples (RNA samples with no reverse transcriptase added) did not amplify across the CT threshold prior to cycle 36, the sample was deemed free of DNA contamination. Illumina's ScriptSeq v. 2 bacterial kit with Ribozero gold; constructed with low-concentration protocol Enzymatic fragmentation, random-hexamer priming for amplification
GEO Accession
GSM2770952