GSM2771031: mac7-1_2_sRNA; Arabidopsis thaliana; ncRNA-Seq
To isolate small RNAs from total RNA (Total RNA was extracted with TRIzol reagent, Life Technologies, USA, 15596018), 50 µg of total RNA from each sample was resolved on 15% urea-PAGE gel, and the 18–30-nt region was excised from the gel. Small RNAs were recovered by soaking the smashed gel in 0.3M NaCl overnight, followed by ethanol precipitation. Small RNA libraries were constructed following instructions from the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (E7300).
GEO Accession
GSM2771031