Amplicons of aquatic mycobacterial hsp65 metagenome: Site N-02 drinking water 2
Mycobacterial hsp65 genes were amplified by PCR using the Tb11/Tb12 primers (Telenti et al., 1993), purified using the QIAquick PCR Purification Kit, quantified by PicoGreen, pooled by equal mass into a single library for 2x250 bp Illumina MiSeq sequencing, and demultiplexed into forward (R1) and reverse (R2) reads by sample