GSM3213837: K562 Endpoint, Replicate 2; Homo sapiens; OTHER
Genomic DNA was harvested from all samples using the Machery-Nagel Blood XL kit. DNA was digested with Mfe1 overnight at 37C, then run on 0.8% agarose big gels to enrich for the lentiviral fragment expected to run at 2158bp. The Machery-Nagel gel extraction kit was to isolate DNA from gel for library construction. The sgRNA- and barcode-encoding regions were then amplified by PCR using custom primers (see Key Resources table in the associated manuscript). Amplified sequences were sequenced on an Illumina HiSeq 2500 or 4000 using custom sequencing primer sets (see Key Resources table in the associated manuscript).
GEO Accession
GSM3213837