GSM3759520: 10dag_PORI_2; Arabidopsis thaliana; ncRNA-Seq
Total RNAs were extracted using TRIzol reagent (Invitrogen) and purified with the Quick-RNA Miniprep Kit (Zymo Research). The RNA integrity number (RIN) of each sample was measured using the Agilent Technologies 2100 BioAnalyzer, and RINs for all the samples were larger than 8. After gel electrophoresis of total RNA by TBE-urea PAGE, small RNAs were purified from the excised gel slices. Small RNA samples were converted to DNA libraries using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs, MA, USA), following the manufacturer's protocol.
GEO Accession
GSM3759520