16S rRNA amplicon sequencing of human metagenome: adult male gut
The hypervariable region V4 of the 16S rRNA gene was amplified using forward primer (515f) and reverse primer (806r). Subsequently, 12 bp unique barcode sequences were attached to each sample for multiplexing. PCR amplification of the V4 region of the 16S rRNA gene was performed according to the Earth Microbiome Project protocol (http://press.igsb.anl.gov/earthmicrobiome/emp-standard-protocols/16s/)