GSM2586721: C57Bl/6 Wild-Type Female Popliteal Lymph Nodes; Mus musculus; RNA-Seq
Whole tissues were homogenized and RNA was extracted by Trizol-Chloroform phase separation. RNA was then purified and concentrated using Qiagen Rneasy Mini columns The same amount of total RNA for each sample was used to prepare libraries using the Kapa Biosystems Stranded library preparation kit with Riboerase. Libraries were labeled with barcodes from a New England Biosciences kit. After library preparation, samples were assessed for quantity and quality by bioanalyzer. Equal amounts of each library were multiplexed and submitted to the facility at University of California, San Francisco, Parnassas, CA for sequencing by Rapid paired end 2x50 on the Hiseq2500. Sequencing was per Illumina (San Diego, CA) protocol in rapid run mode.
GEO Accession
GSM2586721