GSM1636932: MCF7_scRNA_3; Homo sapiens; RNA-Seq
Total RNA was extracted from nuclei using Trizol reagent. Short RNA (25-70nt size range) was selected using 15% urea-TBE PAGE. Capped RNA was selected using sequential enzymatic treatments using 5'-polyphosphatase, 5'-terminator exonuclease, alkaline phosphatase, and tobacco acid pyrophosphatase. Library was subsequently constructed using Illumina TruSeq SmallRNA kit All material was extracted from untreated MCF-7 cells Illumina TruSeq small RNA or ChIP-seq library preparation kits were used to prepare libraries
GEO Accession
GSM1636932