GSM1537831: PAR-iCLIP replicate 1; Drosophila melanogaster; RIP-Seq
5 x 107 S2 cells were treated to 100uM 4-thiouridine 16hours prior to UV-crosslink with 250 mJ at 365nm. 0,05U/µl  ribonuclease T1 was used to trim RNA prior to IP with anti-Imp. The RNA purification and library generation was performed as described in (Konig et al. 2011, Journal of visualized experiments :JoVE). 34 cycles of PCR was used to create the library. See individual experiment
GEO Accession
GSM1537831