GSM3760157: DSSd10_2; Mus musculus; RNA-Seq
Colon samples were stored in RNAlater (Ambion) at -80˚C until further use. Colonic samples were homogenized using bead-beating system (Precellys) for total RNA purification using RNAeasy kit (Qiagen) following manufacturers recommendations. RNA purity and quantity was measured by NanoDrop spectrophotometer (ThermoFisher). All samples were screened for RNA integrity check and presented RIN values above 8 on 2100 Bioanalyzer instrument (Agilent). Samples were submitted to Novogene for library preparation using TruSeq Stranded mRNA Library Prep Kit (poly-A selection) and sequencing using Illumina HISEQ 2500 platform (Illumina). Samples were sequenced using single-end 50bp sequencing, aiming a minimum coverage of 20M reads.
GEO Accession
GSM3760157