GSM1536149: Pi9.ZH10.8.1-RNAseq; Oryza sativa; RNA-Seq
Total RNAs were isolated using TRIzol reagent (Life Technologies) and treated with RNase-free DNase I (Takara) to remove any contaminating genomic DNA. mRNA extraction was performed using Dynabeads oligo(dT) (Dynal; Invitrogen Corp.). Double-stranded cDNAs were synthesized using reverse transcriptase (Superscript II; Invitrogen Corp.) and random hexamer primers. The cDNAs were then fragmented by nebulization, and the standard Illumina protocol was followed thereafter to create the mRNA-seq libraries.
GEO Accession
GSM1536149