MLV integration site junctions in human CD34+ cells
Human CD34+ cells were infected with MLV vector for 4 days.  Genomic DNA was purified and sheared to 300-500bp.  And then end repaired and A tailed.  A partially double stranded linker with a T overhang was ligated to the fragmented genomic DNA.  MLV integration junctions were amplified by PCR using one primer specific for the MLV-LTR and one primer specific for the linker.  Illumina sequencing primers and adaptors were attached with nested PCR.  The PCR products were sequenced on Illumina HiSeq with 2x105bp PE kit.