GSM1534024: SLK_polyA_mRNAseq_rep3 [mRNA]; Homo sapiens; RNA-Seq
Total RNA was extracted from cells using miRVana miRNA isolation kit (Ambion, Life Technologies) according to manufacturer instructions. RNA abundance and integrity were determined after isolation using a Nanodrop-ND-1000 spectrophotometer (Thermo Fisher Scientific) and an Agilent 2100 Bioanalyzer, respectively. For mRNA-Seq: Total RNA of samples used for small RNA-Sequencing was treated with Turbo DNA-free DNase I and Dynabeads to deplete samples from the residual DNA and to isolate the polyadenylated mRNA transcriptome, respectively. PolyA+ RNA libraries were then prepared with the ScriptSeq v2 RNA-Seq kit (Epicentre). The final concentration and size distribution of the RNA libraries were measured by using a Nanodrop-ND-1000 spectrophotometer, and by running a DNA 100 chip on an Agilent 2100 Bioanalyzer. Finally, a total of 6 polyadenylated mRNA libraries (3 SLK and 3 SLKK samples) were sequenced using the Illumina HiSeq platform.
GEO Accession
GSM1534024