Rhizospheric rep 2
The libraries were prepared using Nextera DNA Flex library preparation kit (Illumina) following the manufacturer's user guide. The initial concentration DNA was evaluated using the Qubit dsDNA HS Assay Kit (Life Technologies). The DNA  was used to prepare the libraries.  The samples underwent the simultaneous fragmentation and addition of adapter sequences that were utilized during a limited-cycle PCR . The final concentration of the libraries were measured using the Qubit dsDNA HS Assay Kit (Life Technologies), and the average library size was determined using the Agilent 2100 Bioanalyzer (Agilent Technologies). The libraries were pooled and diluted (to 0.6nM) and sequenced paired end for 300 cycles using the NovaSeq system (Illumina).