The effect of estrogen and progesterone and their antagonists in Ishikawa cell line compared to MCF7 and T47D cells
The effect of estrogen and progesterone and their antagonists in Ishikawa cell line compared to MCF7 and T47D cells
Ishikawa and MCF7 ells were grown in DMEM medium, supplemented with 10% FBS and 1% penicillin/streptomycin; T47D cells were grown in RPMI 1640 medium, supplemented with 10% FBS and 1% penicillin/streptomycin. 10 -8M estrogen and progesterone; 10 -6M Tam, ICI, RU Total RNA was extracted in addition to untreated cells at four different time points - 3h, 6h, 12h and 24h using an RNeasy Mini Kit (Qiagen).
Experimental Factor: COMPOUND
tamoxifen
Experimental Factor: DOSE
10 ^ -6 M
Experimental Factor: TIME
3 hours
Experimental Factor: CELL_LINE
Ishikawa