GSM1314108: c587-gal4>UASt-HA::Lsd1_input; Drosophila melanogaster; ChIP-Seq
Lysates were clarified from sonicated samples and the Lsd1-DNA complexes were isolated using an anti-HA antibody Libraries were prepared according to SOLiD 5500 instructions accompanying the ChIP-seq kit with barcoding beta protocol. AMPure® XP beads were used in this protocol. Briefly, DNA was end-repaired using a combination of 5X reaction buffer, dNTP mix (10mM), end polishing enzyme 1 and 2, and nuclease free water included in the kit. Binding and washing of end-paired DNA was performed using AMPure® XP beads and 70% ethanol respectively. Then, the end-paired DNA was eluted with elution buffer included in the kit. Then,  the 5500 barcoded adaptors were liagted to the DNA and purified using 70% ethanol. Purified adaptor ligated DNA was PCR amplified for 10-15cycles. Amplified DNA was purified and ~1μL of purified amplified product was run on a 2% E-Gel® EX Gel to confirm the removal of any primer dimers.This product was quantified using qPCR. Following qPCR quantitation, the SOLiD™ ChIP-Seq fragment library was subjected to templated bead preparation.
GEO Accession
GSM1314108