GSM4681461: Lib_1B_CD127p; Homo sapiens; RNA-Seq
Cells were sorted in 10 µL PBS 1X (Sigma-Aldrich), spun down immediately and stored frozen at -80°C in a maximum volume of 7 µL PBS 1X (Sigma-Aldrich). Library preparation was performed by starting directly from   ̴500 cells per sample using the SMART-Seq Stranded Kit (Clontech-Takara) and following the manufacturer's protocol.  Libraries were qualitatively assessed by using TapeStation 4200, quantified by Qubit Fluorimeter, then multiplexed in equimolar pools and sequenced on a NextSeq-550 Illumina Platform, generating on average 32.9 million 75bp-PE reads per sample .
GEO Accession
GSM4681461