GSM2155012: HCT116_Hyp_2hr_rep3; Homo sapiens; RNA-Seq
RNA was extracted using the Qiagen QIAshredder kit (79654) and the Qiagen RNeasy Mini Kit (74104) as per the manufacturer's instructions. The RNA was DNase treated following the protocol in the RNeasy Mini Kit with Qiagen RNase-free DNase I (79254). Indexed PolyA libraries were prepared using 1ug of Total RNA and 13 cycles of amplification in the NEB Next Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs Inc., Cat No: E7420S). Libraries were quantified by qPCR using a Kapa Library Quantification Kit for Illumina sequencing platforms (Kapa Biosystems Inc., Cat No: KK4835). Pooled libraries were clustered at 15pM on the cBot and 2 x 100bp sequencing was carried out using the High Throughput mode of a HiSeq 2500 using TruSeq SBS Kit v3 chemistry (Illumina Inc.)
GEO Accession
GSM2155012