GSM4568618: 15hr_DKO_vitc_hmedip; Mus musculus; OTHER
Frozen pellets were treated with 1% 2-mercaptoethanol in Qiagen Buffer RLT Plus to lyse the cells. DNA was separated using the Qiagen AllPrep DNA spin columns, and the flow-through was used for RNA purification. DNA extraction was completed using the Qiagen DNeasy kit. RNA was purified as per the mirVana miRNA isolation kit (ThermoFisher AM1560). We constructed RNA-seq, meDIP-seq, and hmeDIP-seq libraries as described previously (Mingay et al., 2017).  Illumina sequencing libraries were generated by end repair, 3' A-addition, and Illumina sequencing adaptor ligation.
GEO Accession
GSM4568618