Illumina HiSeq 2500 sequencing; Comparing the transcriptome profiling of ob/ob mice treated with the GLP-1 receptor agonist liraglutide, a clinically used GLP-1 receptor agonist, versus baseline controls
Comparing the transcriptome profiling of ob/ob mice treated with the GLP-1 receptor agonist liraglutide, a clinically used GLP-1 receptor agonist, versus baseline controls
All animals had free access to food and water during the experiment. Vehicle or Liraglutide at a dose of 600 µg/kg/d were continuously infused (at a dose of 600 µg/kg/d) over a 14 days period. RNA was isolated from individual WAT samples, homogenized in 1ml trizol and 200µl chloroform. Aqueous phase of 500µl was obtained and mixed with 500µl of Isopropanol. After centrifugation, RNA pellets were washed trice in 80% Ethanol and finally stored in 30µl of DEPC-water. Total RNA was prepared with the Illumina RNA Sample Prep Kit to deliver a mRNA-focused library.
Experimental Factor: compound
vehicle