GSM4682872: xenograft SH-SY5Y koGATA3 #1 rep2 H3K27ac, ChIP-seq; Homo sapiens; ChIP-Seq
Chromatin preparation and ChIP were performed with Ideal ChIP-seq kit for histones according to the supplier's protocol (Diagenode ) llumina sequencing libraries were prepared from ChIP and input DNAs using the TruSeq ChIP library preparation kit according to the manufacturer's protocol. Briefly, DNA were subjected to consecutive steps of end-repair, dA-tailing and ligation to TruSeq indexed Illumina adapters. Size-selection was performed for the H3K27ac ChIP (100 – 600 bp). After a final amplification step, the resulting DNA libraries were quantified using a qPCR method (KAPA library quantification kit) and sequenced on the Illumina NovaSeq6000 instrument (single reads 100 nts).
GEO Accession
GSM4682872