C6h
Tissue from three individual maize plants was combined into one experimental replicate, and four replicates were collected for each time point. The purified total RNA (23 g) was used for the preparation of strand-specific RNAseq libraries (Zhong et al., 2011; Chen et al., 2012) and amplified for 16 cycles. The purified RNAseq libraries were quantified, and 20 ng of each was used for next-generation sequencing using an Illumina HiSeq2000 instrument (Illumina, San Diego, CA, USA) at Weill Cornell Medical School (New York, NY, USA) with a 101 bp single-end read length. Libraries were multiplexed and sequenced in one lane.