GSM4508695: 1436476_Foxp3_PSI_1; Mus musculus; ChIP-Seq
For Foxp3-PSI ChIP, cell fixation and intracellular staining was performed with Foxp3 staining kit (eBioscience). After Foxp3 PSI reaction, cells were fixed with 1% methanol-free formaldehyde at room temperature for 5 min and then sonicated for enrichment of biotinylated chromatins with streptavidin beads. Precipitated DNA was released by proteinase K digestion, phenol: chloroform extraction, and 2-propanol precipitation. Traditional Foxp3 ChIP was conducted according to standard protocols (PMID: 23021222). Libraries were constructed according to standard protocols for ChIP
GEO Accession
GSM4508695