Shotgun:Marine sediment:Tasmania
Refrigerated sediment, EDTA  incubation, 2uL library input, blunt-end repair, adapter ligation, Bst  fill-in reaction, 1st amplificiation (IS7/IS8) with 13 cycles, 2nd  amplification (IS4/GaII-Index) with 13 cycles, cleanup with beads,  Tapestation quantification