GSM2341987: Collapsed Lungs; Bos taurus; miRNA-Seq
Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer.s procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0. Approximately 1 ug of total RNA were used to prepare small RNA library according to protocol of TruSeq Small RNA Sample Prep Kits(Illumina, San Diego, USA). RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM2341987