GSM4681847: AtHB25-A; Arabidopsis thaliana; ChIP-Seq
Transgenic proUBQ10-AtHB25-3xHA siliques in maturation stage (ranging from 7 days-after pollination (DAP) to 14 DAP) were collected and gently ground. They were filtered using liquid nitrogen to obtain high seed enrichment (5 grams approximately). The ChIP protocol was performed as described by Gendrel et al. (2005). Mouse monoclonal antibody HA.11 (clone 16B12) COVANCE and Dynabeads® Protein G were used for the immunoprecipitation. An equivalent sample with no antibody was used as a negative control. The presence of HB25:HA after the immunoprecipitation with antiHA:HPR antibody was confirmed by immunodetection. Two ChIP replicas and the sample without antibody were sequenced with the 1x50 bp HiSeq2500 by the Genomic Unit of CRG (Barcelona, Spain). NEBNext® Ultra™ DNA Library Prep Kit for Illumina®
GEO Accession
GSM4681847