GSM4233386: RNA_mOocy_b57; Mus musculus; RNA-Seq
The ovary tissues were cut into pieces and then treated with 1ml M2 medium (Sigma-Aldrich) with 50 μL collagenase IV at 37oC for 20 minutes to remove interstitial tissues, rocked samples for 30 minutes. The tissues were resuspended in 1ml M2 medium. Next, needles were used to isolate single oocyte or somatic cells under stereomicroscope. First, hand-picked single cells from mice ovaries were placed in single-cell lysis buffer prepared in advance, and single-cell cDNA was synthesized. Then, the obtained cDNA was purified using XP magnetic beads and DNA Clean and Concentrator Kit-5 (Zymo) and quantified. After that, the cDNA was interrupted into 200bp short fragments after being ligated to the biotin index sequences. Finally, RNA-seq libraries for the purified biotin-labled DNA fragments constructed following the NEB DNA Library Prep kit instructions were sequenced on the Illumina HiSeq X-Ten platform.
GEO Accession
GSM4233386