GSM4232684: embryo (8-cell stage), MUT.RME805.P226; Mus musculus; RNA-Seq
Embryos were individually snap frozen in 4 ul lysis buffer containing dNTP mix, oligo-dT primer and SUPERase-In RNase inhibitor (Invitrogen). RNA-seq library preparation was performed according to the Smart-seq2 protocol with slight modifications. Embryos were isolated and processed in two separate batches. To pre-screen cDNA samples for downstream library preparation, mutant (Prdm10Δ/Δ) and control (Prdm10+/+, Prdm10Δ/+) embryos were identified by qRT-PCR detection of Prdm10 exon 5. 16 cycles of PCR pre-amplification were carried out for batch 1 (P200) and 13 cycles for batch 2 (P226). 1 ng of cDNA was used for tagmentation with the Nextera XT DNA Library Prep Kit (Illumina). Final libraries were amplified and pooled for single-end Illumina sequencing.
GEO Accession
GSM4232684