GSM2497829: PkcdRed-4; Mus musculus; RNA-Seq
Mice were decapitated, brains extracted on ice and 1mm thick brain section cut in ice-cold Hibernate A Low Fluorescence solution (BrainBits). The central amygdala was extracted using biopsy punchers (1mm diameter; Integra Miltex) and enzymatically dissociated (Papain Dissociation System, Worthington Biochem). Approximately 1000 td-Tomato+ cells were retrieved with subsequent FACS sorting. Libraries were prepared with the SMARTer® smRNA-Seq Kit for Illumina® (Clontech, 78100 Saint-Germain-en-Laye, France) and submitted to deep sequencing on a HiSeq 2500 system (Illumina, San Diego, USA).
GEO Accession
GSM2497829