GSM3258248: 160217_Prmt5_ff: Prmt5-fl/fl_Replicate 2; Mus musculus; RNA-Seq
Trizol reagent (Invitrogen) was used with the following modifications: phase separation was repeated to minimize gDNA carryover, ethachinmate (Wako) was used to facilitate RNA precipitation, and RNA wash was repeated to minimize carryover of salts/organic contaminants. SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Bio) was used for cDNA synthesis and amplification of mRNA. Library preparation was performed using Nextera® XT DNA Library Preparation Kit (Illumina).
GEO Accession
GSM3258248