GSM2341165: Slc7a5-KO replicate 2; Mus musculus; RNA-Seq
Mice were euthanized under anesthesia and the brains were quickly dissected on ice. The cerebellum was used for RNA extraction by using 700 μL Trizol (Invitrogen) for homogenization and 140 μL chloroform (Sigma), followed by centrifugation at 12000g for 15 min at 4°C. The upper aqueous phase was transferred to a new tube and 1.5 volumes of 100% ethanol were added. Total RNA was extracted by using the RNA Clean&Concentrator-5 prep Kit (Zymo Research). DNase I (Promega) treatment was also applied as described in the kit instructions manual. RNA sample quality was checked by using the NanoDrop spectrophotometer (Thermo Scientific) and the Bioanalyzer 2100 with the RNA 6000 Nano kit (Agilent). 1.5 μg total RNA were used to generate cDNA libraries by following the manufacturer’s instructions in the SENSE mRNA-Seq Library Prep Kit V2 (Lexogen). Quality check of the generated libraries was assessed by using the Bioanalyzer 2100 with the High Sensitivity DNA Analysis Kit (Agilent).
GEO Accession
GSM2341165