GSM3244295: B2; Streptomyces natalensis; RNA-Seq
Total RNA was extracted using Total RNA Extractor（Trizol） (Cat# B511311, Sangon Biotech)following the manufacturer's instructions and checked for a RIN number to inspect RNA integrity by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US).Qualified total RNA was further purified by Qubit2.0 RNA Kit (Cat Q32855,Life). Library was prepared according to Illumina's instructions with VAHTS Total RNA-Seq（H/M/R) Library PrepKit (NR603-02, Vazyme）. Briefly, RNA was extracted and rRNA was depleted with Ribo-Zero rRNA Removal Reagents(MRZB12424, Epicentre). mRNA was fragmented with Fragment High Mix. First Strand cDNA was synthesized with First Strand Synthesis Act D Mix and Second Strand cDNA was synthesized with Second Strand Marking Master Mix. Adenylate 3' Ends and Ligate Adapters were treated. After adapter ligation DNA was PCR amplified with Illumina primers for 15 cycles and library fragments of 520 bp were checked with an Agilent 2100 Bioanalyzer. Libraries were sequenced on Illumina Hiseq 2000 System following the manufacturer's protocols.
GEO Accession
GSM3244295