GSM1312897: Set B mut-14(pk738) rep2; Caenorhabditis elegans; RNA-Seq
Total RNA was isolated from stage-matched animals by Trizol extraction followed by two rounds of chloroform extractions and precipitated using isopropanol. Small RNAs were size selected, tobacco acid pyrophosphatase (TAP) treated to remove 5’ di- and triphosphate groups, ligated to 5’ and 3’ adapters, subjected to reverse transcription, amplified by PCR, and gel purified in preparation for sequencing.
GEO Accession
GSM1312897