GSM1641222: Af_T08_2; Drosophila melanogaster; RNA-Seq
Total RNA extraction and DNase I digestion were performed using the MasterPure RNA Purification Kit (Epicentre) Poly-A mRNA was purified and fragmented by sonication. First strand single end cDNA was synthesized using random primers. Eight tagged libraries were generated, pooled and run on two lanes of a HiSeq 2000 sequencer (Illumina) to generate single reads of 50 bases
GEO Accession
GSM1641222