scRNA-seq of mus musculus: neonatal mouse stomach explants
Cell suspensions were loaded on a GemCode Single Cell Instrument to generate single cell Gel bead in Emulsions (GEMs). Upon encapsulation, cells lysis benign and gel beads automatically dissolve to release their oligonucleotides for reverse transcription of poly-adneylated RNAs. Each cDNA molecule contains a UMI and shared barcode per GEM after RT. Subsequently, the emulsions were broken and barcoded cDNAs were pooled for PCR amplification. Amplified cDNAs were shared to ~ 200bp using a Covaris E210 system. After end repair and A-tailing, adapters were ligated to product, followed by PCR to incorporate sample indices.