16S rRNA sequences of Amazonian upland forest soils
Genomic prokaryotic DNA of the V4 hypervariable region of the 16S rRNA gene was amplified using the barcoded primers 515f/806r (Caporaso et al., 2011), specific for Bacteria. Paired-end sequencing, with 2x250 bp reads, was performed in Illumina Hiseq 2500 platform, at Novogene Bioinformatics Technology (Beijing, China), using standard procedures.