GSM1638735: RZB0003 Female; Danio rerio; RNA-Seq
Brain from Male and female fish were extracted for RNA extraction. Brain tissues from 5 fish were collected and pooled. RNA-Seq library preparation protocol: Total RNA was extracted using TRIzol® Reagent (Invitrogen, USA). RNA concentrations were determined using NanoDrop 2000 (Thermo Scientific). The integrity of RNA samples was determined using an Agilent RNA 6000 Nano chip on an Agilent 2100 Bioanalyzer. Samples with RNA Integrity Number (RIN) value of 8-9 was used for RNA seq library preparation. Messenger RNA sequencing library was prepared using TruSeq total RNA sample preparation kit (Illumina; Total RNA sample preparation guide) as per the manufacturers instructions with 3 microgram input RNA per library. Quality of RNA seq library was checked following company recommended protocols. RNA sequencing of male and female samples were performed on the Illumina HiSeq2000 (Illumina, USA) (one sample per lane) machine with single ended 51 bp read length.
GEO Accession
GSM1638735