GSM2424300: run1692_lane12_read1_indexN711-S504=Jul25_Cell_60; Mus musculus; RNA-Seq
B6 X CAST small pre-B cells were FACs sorted for CD19+B220+IgM-CD43low, and used for bulk or single cell sorting and sequencing. Bulk sorted CD19+B220+IgM-CD43low small pre-B cells were single cell sorted on pre-primed C1 Fluidic Chips. Cells were lysed, RT performed (Clontech SMARTer) and cDNAs transferred to 96 well plates. Libraries were prepared using Nextera XT (Illumina) then tagmented. Pooled libraries were purified with AMPure magnetic separation assayed for quality with Qubit® dsDNA HS (Life Tecnologies) and sequenced (75bp paired).
GEO Accession
GSM2424300