16S rRNA amplicons of human faecal batch incubation: donor 1, pH 5.5, long-chain inulin
PCR amplification of V1-V3 region of 16S rRNA gene from human faecal DNA with primers containing Roche adaptors and reverse primer containing 12 nucleotide long unique barcode. Quadruplicate PCR amplicons were combined and gel-purified. 50 samples were pooled and sequenced on a GS FLX 454 platform.