Illumina HiSeq 2500 paired end sequencing; Pig transcriptome annotation and gene expression atlas
TruSeq Stranded mRNA Sample Preparation Guide Revision E
assay type
transcription profiling by high throughput sequencing
sample storage
RNAlater, frozen
sample storage processing
cryopreservation, other
sampling to preparation interval
3
experimental protocol
http://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf
extraction protocol
ftp://ftp.faang.ebi.ac.uk/ftp/protocols/samples/ROSLIN_SOP_Isolation_of_RNA_from_RNAlater_preserved_tissue_20161108.pdf
library preparation location
Edinburgh Genomics, The University of Edinburgh, EH9 3FL, Edinburgh, Scotland
library preparation location longitude
-3
library preparation location latitude
56
library preparation date
2013-10-15
sequencing location
Edinburgh Genomics, The University of Edinburgh, EH9 3FL, Edinburgh, Scotland
sequencing location longitude
-3
sequencing location latitude
56
sequencing date
not applicable
experiment target
total RNA
rna preparation 3' adapter ligation protocol
http://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf
rna preparation 5' adapter ligation protocol
http://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf
library generation pcr product isolation protocol
http://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf
preparation reverse transcription protocol
http://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf
library generation protocol
http://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-guide-15031048-e.pdf
read strand
mate 2 sense
rna purity - 260:280 ratio
1.96
rna purity - 260:230 ratio
1.32
rna integrity number
8.1