GSM1420383: siGFP-PA-rep2; Drosophila melanogaster; RNA-Seq
mRNA from siRNA transfected OSCs was selected with Dynabeads Oligo(dT) (Invitrogen) from total RNA, fragmented and reverse transcribed with random hexamers. Strand-specific libraries were prepared using the UDG-digestion-based strategy and cloned with NEBNext® Ultra™ DNA Library Prep Kit for Illumina (NEB) total RNA was isolated with Trizol see in the SAMPLES section
GEO Accession
GSM1420383