RNA-seq of E. piscicida CK108
Ribosomal RNA from the purified RNA (RIN > 8) was removed by the Anydeplete probe (Nugen) and the remaining RNA was used to construct sequencing library by Truseq Stranded Total RNA H/M/R Prep kit (Illumina) according to the manufacturers recommendations. The quality of the amplified libraries was verified by capillary electrophoresis (Bioanalyzer). NovaSeq 6000 (Illumina) platform was used for the sequencing and 100-bp of pair-end reads were generated (20-30 million reads per sample).