GSM3328868: RC17_before_11_H7; Homo sapiens; RNA-Seq
Single cells have been extracted from tissue using Papain Dissociation System (Worthington). cDNA was generated from FACS sorted single cells (ventral midbrain human embryonic stem cells before grafting, fetal cells before and after grafting were sorted for size, ventral midbrain human embryonic stem cells after grafting were sorted for GFP expression) using Smart-seq2 protocol (Picelli et al. Nature Methods 2013).  ERCC spike-in RNA was added to the lysis buffer. Nextera XT DNA sample preparation index kit, 96 indices (Illumina)
GEO Accession
GSM3328868