GSM2770712: CRISPR_screen_THP-1 Day 21- Biorep 3; Homo sapiens; OTHER
At each time-point, cells were spun down, washed with PBS, flash frozen on dry ice, and genomic DNA was harvested with Qiagen DNeasy Kits and sgRNAs were amplified by PCR. The ~221 bp sgRNA products were isolated. NGS libraries were prepared with NuGen Ovation low complexity kits, followed by PCR amplification for 6 cycles. 6pM of samples were sequenced on an Illumina MiSeq with reagent kit v3 for 150 cycles.
GEO Accession
GSM2770712