GSM3176560: ChIP-seq of HA in JMJ16-HA; Arabidopsis thaliana; ChIP-Seq
Total RNA was extracted from 5-6 rosette leaves with TRIZOL (RNAiso Plus TaKaRa). 1 g of leaves 5 and 6 of indicated developmental stages of Col-0, jmj16-1 and transgenic jmj16-1 plants expressing JMJ16 full length driven by its own promoter were harvested and ground in liquid nitrogen and then cross-linked with formaldehyde. Anti-HA (Sigma H6908) and anti-H3K4me3 (Millipore 07-473)  were used for immunoprecipitation. RNA libraries were prepared for sequencing using standard Illumina protocols using 1 mg RNA. A ChIP-seq library was constructed using the NEBNext® DNA Library Prep Master Mix Set for Illumina® (NEB, E6040S).
GEO Accession
GSM3176560