GSM2425536: Drosophila melanogaster w1118 testis total RNA replicate 1; Drosophila melanogaster; RNA-Seq
Total RNAs were isolated from w1118, Tailor, Dis3L2, and Tailor/Dis3L2 mutant 5 day old fly testes using Trizol. Two independent dissections were made to generate biologically replicate RNA samples, whose quality was assessed by Bioanalyzer The Illumina Truseq Total RNA library Prep Kit LT was used to make RNAseq libraries from 650 ng of total RNA. Manufacturer’s protocol was followed except for using 8 cycles of PCR to amplify the final library instead of the recommended 15 cycles, to minimize artifacts caused by PCR amplification. All samples were pooled together, using the barcoded adapters provided by the manufacturer, over 2 flow cells of a HiSeq2500 and sequenced using pair-end PE50 at the New York Genome Center.
GEO Accession
GSM2425536