GSM4633332: Untreated fibroblasts cultured on low elastic modulus plates; Homo sapiens; RNA-Seq
Cells were washed with phosphate-buffered saline before detachment using trypsin-EDTA (Sigma-Aldrich) and collection in fresh growth medium. Following centrifugation at 1500 rpm for 5 minutes, the resulting cell pellet was suspended in cell suspension buffer (90% ddH20, 9% Optiprep (Sigma), 1% PBS with 0.1% BSA) Libraries were prepared for sequencing as per the Drop-seq and standard Illumina protocols
GEO Accession
GSM4633332