ChIP-Seq of PAO1 QscR overexpression: PAO1 qscR- B
DNA was crosslinked to transcription factors with 1% formaldehyde and fragmented using a sonicator. The size of fragments was confirmed to be in the 300-1000 bp range by agarose gel electrophoresis. Fragmented DNA was immunoprecipitated using a 50% protein A Sepharose slurry with purified anti-QscR antibody. Cross-links between the protein and DNA were reversed by incubating the eluate at 65C overnight. DNA was purified using phenol-chloroform-isoamyalcohol extraction and ethanol precipitation, and then further purified using a cleanup kit. DNA was sequenced using an Illumina MiSeq.