GSM4632833: UC-35 FBs; Homo sapiens; RNA-Seq
For both fibroblasts and induced organoids  nuclei isolation, we used 2 ml of ice-cold EZ PREP buffer to homogenize the cell pellets with a glass dounce tissue grinder, x20 for each loose and tight pestle  and incubated on ice for 5 min, with an additional 2 ml of ice-cold EZ PREP. Next, nuclei were centrifuged at 500 × g for 5 min at 4 °C and washed with ice-cold EZ PREP and filtered through a 40μm cell strainer. After centrifugation, the nuclei were washed in 4 ml of nuclei Suspension Buffer (NSB; consisting of 1× PBS, 0.01% BSA and 0.1% RNase inhibitor) and filtered through a 20μm cell strainer and proceeded with the 10x genomic pipeline for sequencing. Followed the 10X genomics pipeline
GEO Accession
GSM4632833