GSM4194752: ChIPseq-LEVER_KD-Input; Homo sapiens; ChIP-Seq
1) RIP-seq: Cells were fixed with 1% formaldehyde. RNA was sonicated to around 3000 and protein-RNA complexes were pulled down by antibodies. 2) RNA-seq: Fractionated RNA was harvested by Trizol followed by DNaseI treatment. 3) NANOPRE-seq: Fractionated RNA was harvested by Trizol followed by DNaseI treatment. Superscript IV was used for nuclear cDNA preparation. 4) ChIP-seq: Cells were fixed with 1% formaldehyde and insoluble chromatin fraction was isolated. Chromatin was sonicated to 100 to 500 base pair and protein-DNA complexes were pulled down by antibodies. 5) 4C-seq: Cells were fixed with 2% formaldehyde. gDNA was digested first with DpnII and ligated. The ligated chromatin was digested again with NlaIII, MseI or MluCI and ligated. 1) ChIP-seq: Libraries were prepared using ThruPLEX DNA-seq kit (R400428) according to manufacturer's instruction. Constructed libraries were PAGE purified and only libraries with 250 to 500bp were used for sequencing. 2) RIP-seq and RNA-seq: Libraries were prepared using Scriptseq V2 RNA-seq Library preparation kit (SSV21124) according to manufacturer's instruction.  Constructed libraries were PAGE purified and only libraries with 250 to 500bp were used for sequencing. 3) NANOPORE-seq:  Library for nuclear cDNA was constructed with 1D cDNA library kit (SQK-LSK108) according to manufacturer's instruction. RNA library for total polyA+ RNA was constructed with direct-RNA sequencing kit (SQK-RNA001) according to manufacturer's instruction. 4) 4C-seq: 4C library was constructed with customized primers with Illumina adaptor and barcodes and KAPA HiFi HotStart ReadyMix (KK2602).
GEO Accession
GSM4194752