GSM3757272: Heatin_2d_Control_1_M_TOPHAT.norm.txt; Arabidopsis thaliana; RNA-Seq
Two day-old or 7d day-old seedlings were harvested at the moment of dawn (lights on), and snap frozen in liquid Nitrogen. RNA was isolated using the Sigma Spectrum Plant Total RNA isolation kit and gDNA was removed by on-column DNAse treatment (Sigma-Aldrich). RNA integrity and concentration was checked using RNA 6000 Nano Chips on a Bioanalyzer (Agilent-2100). For RNA-seq library preparation in total 3 samples were prepared for each genotype, treatment and time-point, by combining isolated RNA of 3 individually harvested batches per sample. Illumina TruSeq RNA Library preparation and Illumina HiSeq2500 (high-throughput) single-end 50 bp sequencing was outsourced to Macrogen, Korea.
GEO Accession
GSM3757272