CAGE of human: hepatoblastoma tumor: Sample 229T
Total RNA was used to synthesize cDNA with random primers. Full-length cDNAs were biotinylated and captured by streptavidin-coated magnetic beads. The cDNAs were released from cap-trapped RNAs, ligated to 5' linkers including barcode sequences, and digested with EcoP15I. The detailed protocol is described in Ref [PMID:22362160]
alignment_software
bwa v.0.5.9