GSM1536175: BRD4 ChIP; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde. After wash with PBS, the cells were lyzed in ChIP lysis buffer. Lysates were sonicated and cleared by centrifugation. After pre-clear with protein A beads, antibody (Bethyl Labs A301-985A lot A301-985A-1) was incubated with the lysate overnight at 4°C. Protein A was used to capture the protein DNA complex. The eluted protein DNA complexes were reverse crosslinked and DNA was purified by a Qiagen PCR purification column. Libraries were prepared using ChIP-Seq Library Prep Reagent Set for Illumina from NEB. Briefly, DNA was end-repaired. 3' A base was added and ligated to adaptors. After PCR amplification, DNA was purified and analyzed with Illumina HiSeq.
GEO Accession
GSM1536175