NextSeq 500 sequencing; RNA-Seq of RAW 264.7 cells stimulated with the AS03 adjuvant after 4 and 8 hours
RNA-Seq of RAW 264.7 cells stimulated with the AS03 adjuvant after 4 and 8 hours
10e6 RAW 264.7 cells were cultured in medium (DMEM containing 10% of FCS, L-glutamine, pyruvate, non-essential amino acids, and penicillin/streptomycin/gentamycin) in 12 well plates at 37°C with 5% of CO2 overnight.  Cells were then stimulated with AS03 (1/30) for 4 or 8 hours in independent triplicates. total RNA was extracted with an RNAeasy Plus Mini Kit (QIAGEN) following manufacturer's instructions. The RNA concentration and purity were measured with a NanoDrop spectrophotometer. RNA libraries were prepared for sequencing using Illumina Truseq Stranded mRNA library prep kit
Experimental Factor: compound
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Experimental Factor: time
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