GSM2771065: BM277; Homo sapiens; RNA-Seq
We extracted total RNA from pre-treatment bone marrow mononuclear cells using TRIzol (Thermo Fisher Scientific, Inc., Wilmington, Delaware, USA).  RNA samples were purified by using Qiagen RNeasy Mini kit and DNase I (Qiagen, Valencia, CA, USA).  The mRNA contents were enriched by using oligo (dT)-labeled magnetic beads, followed by fragmentation, conversion into cDNA, ligation of sequencing adaptors, and amplification.  All procedures were performed according to manufacturer's instructions. Library was constructed according to Illumina's instructions. Quality-checked library products were subjected to 75-bp paired-end sequencing by an Illumina NextSeq 500 sequencer (Illumina Inc., San Diego, CA, USA) with a throughput of approximately 20 million reads per sample.
GEO Accession
GSM2771065