GSM4508638: Cal27_KO1_RNA; Homo sapiens; RNA-Seq
Total RNA was extracted from cell pellets of approximatively 1 million cells, washed with PBS, spun down and preserved at -80, using the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen) according to the manufacturer's instructions including DNAse treatment option. Library preparation was performed with ribosomal RNA depletion according to the manufacturer's instructions (NEB) to achieve greater coverage of mRNA and other long non-coding transcripts.
GEO Accession
GSM4508638