GSM3757238: WT_Rpb2_F; Mus musculus; ChIP-Seq
ChIP-Seq sequencing libraries were prepared from recovered DNA by blunting, A-tailing, and adapter ligation using barcoded adapters (NextFlex, Bioo Scientific) as previously described (Heinz et al., 2010; Gosselin et al., 2014). Libraries were PCR-amplified for 12-15 cycles and size selected for fragments (225-400 bp) by gel extraction (10% TBE gels, Life Technologies EC62752BOX). Libraries were single-end sequenced for 51 cycles with an average sequence depth >20 million on an Illumina HiSeq 4000 or NextSeq 500 platform (Illumina, San Diego, CA) according to manufacturer's instructions.
GEO Accession
GSM3757238