microRNA and piRNA profiles in normal human testis
microRNA and piRNA profiles in normal human testis
All the collections and usages of human tissues were approved by the Institutional Review Board at First Affiliated Hospital of Anhui Medical University. Testicular tissues were from three cadaver donors (aged 28 to 35 years) from the First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, People's Republic of China. Histological examination showed normal spermatogenesis in all of the three donators. Each patient has at least one healthy child. Total RNAs were extracted from the human testis using TRIzol (Invitrogen). These samples were then subjected to 15% (w/v) denaturing PAGE (polyacrylamide gel electrophoresis) and the small RNA fragments of 18-28 nt were isolated. The small RNA molecules were ligated to 5' and 3' adaptors sequentially and then converted to cDNA by reverse transcription followed by PCR amplifications
Experimental Factor: individual
pool of three men