GSM4568574: WT before treatment with H2O2 1st duplicate; Bacillus anthracis str. Vollum; RNA-Seq
Total RNA was extracted using the RNeasy kit (Qiagen), residual DNA was digested using RNase-free DNase (Qiagen). Libraries were generated using TruSeq RNA Library Prep Kit v2 (Illumina), assessed for correct sizing using a high sensitivity Bioanalyzer DNA chip (Agilent), quantified by qPCR and normalized to 2 nM. Pooling and clustering of libraries were  performed using the Illumina cBot system; 150-bp paired-end sequencing was performed on the Illumina Genome Analyzer IIx system with TruSeq SBS kit v2 reagents.
GEO Accession
GSM4568574