GSM2771685: Ebi3-Treg (CD4+CD25+Thy1.1+Blimp1-) E3; Mus musculus; RNA-Seq
Lymphocytes were isolated from the LN of 35EbiT-Blimp1-GFP mice and enriched for CD4+ T cells using magnetic beads. Then, CD25+CD62LlowThy1.1+Blimp1- and CD25+CD62LlowThy1.1-Blimp1+ Tregs were sorted to a typical purity of >90% using Thy1.1, CD25, CD62L and CD4 Abs.  SMART-SeqTM v4 UltraTM Low Input RNA Kit for Illumina was used with 10 pg-10 ng of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM2771685