GSM2232663: RHBDF1-P_CAPseq; Homo sapiens; Mus musculus; RNA-Seq
Total RNA was extracted using TRI Reagent (Sigma-Aldrich, T9424) and then fragmented using the NEBNext Magnesium RNA Fragmentation Module. Fragmented RNA was cleaned up using RiboMinus Concentration Module (Life Technologies) and the 3’ adapters were ligated using a modified version of the NEBNext Small RNA Library Prep Set for Illumina (Multiplex Compatible). Samples were subjected to a second round of clean up using RiboMinus Concentration Module and then subsequently treated with Calf Intestinal Phosphatase (CIP) and Tobacco Acid Phosphatase (TAP). The reaction was cleaned up using the RiboMinus Concentration Module (Life Technologies) as before, and annealing of the SR RT (reverse transcription) primer and ligation of the 5’ adapter was performed. Reverse transcription was carried out using the Superscript III First Strand Synthesis system, followed by library amplification and cleaned up using a QIAquick PCR Purification kit (Qiagen, 28104).
GEO Accession
GSM2232663