GSM4681484: 15 min rep 1 [RNA-Seq]; Medicago truncatula; RNA-Seq
For each RNA extraction, roots from 7 plants were pooled and ground while keeping the sample frozen. RNA extraction was performed as described previously (DOI: 10.1007/BF02670468) Libraries were prepared using 1 μg of RNA in the NEBNext® Ultra™ Directional RNA Library Prep Kit following the supplier's instructions (New England Biolabs, Ipswich, MA, USA). Sequencing was carried out with an Illumina HiSeq3000 (2×100 cycles) at the Interdisciplinary Center for Biotechnology Research at the University of Florida (Gainesville, FL, USA).
GEO Accession
GSM4681484