GSM4681637: media1A1: microglia medium control_rep2; Mus musculus; RNA-Seq
Microglia were isolated under ice cold conditions. Pure populations of microglia were obtained by cell sorting for live CD11b positive, CD45 intermediate cells. Sorted cells were collected directly into ice cold lysis buffer and RNA was isolated using the Qiagen RNeasy Micro RNA Kit. Flash frozen brain tissue samples were homogenized and RNA was isolated using the Qiagen RNeasy Mini RNA kit. Takara SMART-Seq v4 Ultra Low Input RNA Kit + Illumina Nextera XT Library Prep Kit
GEO Accession
GSM4681637