archaea of oxidation ditch mm summer
PCR amplification was performed using primers targeting the V1 to V3 regions of the 16S rRNA gene, using the extracted DNA as a template. For bacterial amplification, the barcoded primers 27F5-CCTATCCCCTGTGTGCCTTGGCAGTC-TCAG-AC-GAGTTTGATCMTGGCTCAG-3, (where middleline sequence indicates the target region) and 518R5-CCATCTCATCCCTGCGTGTCTCCGAC-TCAG-X-AC-WTTACCGCGGCTGCTGG-3 (where X indicates the unique barcode for each subject)