Fungi communities in painting surface
Samples were prepared for Illumina Sequencing targeting the Internal Transcribed Spacer 2 region. The DNA was amplified for the hyper variable ITS2 region with specific primers and further reamplified in a limited-cycle PCR reaction to add sequencing adapters and dual indexes. First PCR reactions were performed for each sample using KAPA HiFiHotStart PCR Kit according to manufacturer suggestions, a pool of forward primers: ITS3NGS1_F 5-CATCGATGAAGAACGCAG-3, ITS3NGS2_F 5-CAACGATGAAGAACGCAG-3, ITS3NGS3_F 5-CACCGATGAAGAACGCAG-3, ITS3NGS4_F 5-CATCGATGAAGAACGTAG-3, ITS3NGS5_F 5-CATCGATGAAGAACGTGG-3, and ITS3NGS10_F 5-CATCGATGAAGAACGCTG-3, and reverse primer ITS4NGS001_R 5-TCCTSCGCTTATTGATATGC-3