RAD sequencing willow warbler - isolate 01L/19: Sample ww_01L19
"We followed the RAD protocol in Baird et al. 2008 and Etter et al. 2011 with the following modifications. No RNase A treatment, DNA fragments were sheared with a Bioruptor Standard UCD-200 Diagenode and selected to a size range comprising 150-750 bp length excluding adaptors, PCR reactions were set-up at 50microliter with 16 microliter RAD library template running 16 cycles. We used the restriction enzyme Sbfl and multiplexed 16 samples per library."