Sample from the liquid phase of an  Anaerobic digester treating municipal solid waste
PCR amplification of the 16S rRNA gene  from the DNA extracted from the biofilm and media samples was accomplished using modified  universal bacterial primer pairs F-(5- CCTACGGGNGGCWG- CAG -3) and  R-(5-GACTACHVGGGTATCTAATCC-3) based on  Klindworth et al. (2013), and an Archaea specific primer pair F-  (5-CCCTAYGGGGYGC-ASCAG-3) and R- (5-GGCCATGCACYWCYTCTC- 3) as described  by Gantner et al. (2011)