GSM2154809: NAP_3; Mus musculus; RNA-Seq
Kidneys were harvested from mice and immediately frozen using liquid nitrogen and stored in (-80) until all samples were collected and processed together to extract RNA Total RNA was isolated using the miRNeasy Kit (Qiagen) according to the manufacturer’s protocol. An additional DNase1 digestion step was performed to ensure that the samples were not contaminated with genomic DNA. RNA purity was assessed using the Agilent 2100 Bioanalyzer.  Briefly, total RNA was converted to cDNA using oligodT, which was then used for Illumina sequencing library preparation. Single end 100 bp RNA-seq was carried on an Illumina HiSeq2500.
GEO Accession
GSM2154809