CFIAFB20200081_L. monocytogenes
Brain Heart Infusion broth was inoculated with a single  colony of bacteria and incubated for 14 to 16 h at 35C. Genomic DNA was  isolated from 400 L of culture using the Maxwell 16 Cell SEV DNA  Purification kit (Promega, Madison, WI). DNA was then quantified using the  Quant-iT High-Sensitivity DNA Assay Kit (Life Technologies Inc., Burlington,  ON). Sequencing libraries were constructed from 1 ng of gDNA using Nextera XT  DNA Sample Preparation and Index Kits (Illumina, Inc., San Diego, CA)  according to manufacturers instructions. Genomic sequencing was performed on  the Illumina MiSeq Platform (Illumina, Inc.) using a 600-cycle MiSeq Reagent  kit v3 (Illumina, Inc.).