GSM2155049: ALL#8 CNS; Homo sapiens; RNA-Seq
For samples of ALL#6 and #7, Total RNA was isolated by the RNeasy Kit (Qiagen, Valencia, CA), and cDNA libraries were generated with Ovation RNA-seq System V2 (NuGEN) according to the manufacturer’s instructions. For the sample of ALL#8, RNA extraction and cDNA synthesis of samples were perfomed with SMARTer Ultra Low Input RNA Kit for Sequencing (Clontech Laboratories, Inc. Mountain View, CA, USA) according to the manufacturer’s instructions. Sequencing libraries were constructed using the Nextera XT DNA Sample Prep kit (Illumina) and Nextera XT Index kit (Illumina). For samples of ALL#6 and #7, the libraries were sequenced with the TruSeq SBS kit v3 and TruSeq PE Cluster kit v3 (Illumina) in 100-cycle paired-end mode of HiSeq 2500 (Illumina).   For the sample of ALL#8, The libraries were sequenced with the TruSeq SBS kit v3 and TruSeq SR Cluster kit v3 (Illumina) in 100-cycle Single-Read mode of HiSeq 2500 (Illumina).
GEO Accession
GSM2155049