GSM3245101: 90_mpw_A; Xenopus laevis; RNA-Seq
RNA was isolated using TRIreagent (Sigma) following the manufacturer's protocol. Diluted RNA was treated with DNAse I solution (Sigma), followed by an overnight reprecipitation during incubation at -20 °C using a 4 M final concentration of  LiCl solution . Libraries were prepared from 200 ng of total RNA using the SureSelect Strand-Specific RNA Library Prep for Illumina Multiplexed Sequencing (Agilent, G9691) according to the manufacturer's protocol, which includes a polyA selection of RNA. Sequencing was performed on the NextSeq 550 in 2x75bp HighOutput mode.
GEO Accession
GSM3245101