GSM1536394: Sample R; Neisseria gonorrhoeae; miRNA-Seq
Neisseria gonorrhoeae strain MS11 and the MS11 ΔrppH strain were grown on solid medium for 12 hrs.  Cells were harvested and small RNA was extracted utilizing the MirVana Mira kit; AM 1560 (Life Technologies, Inc) according to the manufacturer's specifications. Transcripts larger than 250-300 bp were excluded from further analysis by size fractionation. Two small RNA libraries (wild type and  ΔrppH) were constructed using the "Small RNA v1.5 Sample Prep Kit" (Illumina).
GEO Accession
GSM1536394