Transcriptomic response of Elodea nuttallii to heavy metals
Transcriptomic response of Elodea nuttallii to heavy metals
Elodea nuttallii were grown in 25-L aquariums supplemented with a layer of sediment in a 16/8 h light/dark cycle at 20C. 5 shoots per treatment were cut and exposed in 1-L PE beakers to 0 (control), 200 ng/L HgCl2, 80 ug/L HgCl2, 1 mg/L HgCl2, 500 ug/L CdCl2 or 5 mg/L CdCl2 for 24 h. The control treatment was run in biological duplicate. Shoots were ground in liquid nitrogen and RNA was extracted using Trizol reagent.
Experimental Factor: COMPOUND
Hg
Experimental Factor: DOSE
0 ng_per_L