GSM2586737: quadAvrRpt2.flg22.120min.rep1; Arabidopsis thaliana; RNA-Seq
Total RNA was extracted from the frozen pulverized leaf tissue using Trizol. Smart-3SEQ, which is a modified 3SEQ method, was used (Foley et al., manuscript in preparation). The first 5 bases of the sequence read is Unique Molecular Identifier (UMI), which helps determining PCR duplicates. RNA-tag-SEQ: The following "GGG" was used for reverse transcriptase template switching. Thus, total 8 bases from the beginning of the read need to be trimmed for an actual mRNA sequence.
GEO Accession
GSM2586737