GSM3258487: 14G-BaP rep 2; Oreochromis niloticus; RNA-Seq
Total RNA was isolated from ~0.5 mg of liver or testis tissues using the Trizol reagent method according to the manufacturer's procedure (Invitrogen™, Carlsbad CA,USA). The RNA pellet was resuspended in 20–50 μl of RNAsecureTM Reagent (Ambion Austin, TX, USA) and DNAse was-treated with Turbo DNA-free (Ambion Austin, TX, USA). RNA cleanup was performed using Phenol-chloroform (1:1) extraction, followed by sodium acetate (3M) and ethanol (100 %) precipitation (Chomczynski et al., 1987). RNA libraries were prepared for sequencing using standard Illumina protocols. Samples were processed following the manufacturer's protocol for NEBNext Ultra™ RNA Library Prep Kit for Illumina (New England, Biolabs (NEB), lpswich, MA, USA) in conjunction with the NEBNext Poly(A) mRNA Magnetic Isolation Module and the NEBNext Multiplex Oligos for Illumina (Index Primers Set 1)
GEO Accession
GSM3258487