Full-length transcriptom
RNA samples of root, stem, young leaf, leaf, flower bud, and flower were mixed together to build Isoform Sequencing library by using Clontech SMARTer PCR cDNA Synthesis Kit and the BluePippin Size Selection System protocol as described by Pacific Biosciences (PN 100-092-800-03), and then sequenced on PacBio Sequel platform. Sequence data were processed using the SMRTlink 7.0 software. Error correction was using the Illumina RNA- seq data with the software LoRDEC, and any redundancy in corrected consensus reads was removed by CD-HIT to obtain final transcripts for the subsequent analysis.