RNA-seq N. benthamiana leaf GFP agroinfiltrated Replicate 2
Indexed libraries were prepared from 1 g/ea purified RNA with TruSeq Stranded mRNA Sample Prep Kit (Illumina) according to the manufacturers instructions. Libraries were quantified using the TapeStation 4200 and pooled such that each index-tagged sample was present in equimolar amounts, with final concentration of the pooled samples of 2 nM. The pooled samples were subject to cluster generation and sequencing using a NextSeq 500 System (Illumina) in a 2x75 paired end format at a final concentration of 1.8 pmol.