GSM1309661: wt_sRNA; Schizosaccharomyces pombe 972h-; RNA-Seq
Cells were disrupted and total RNA was isolated by grinding frozen cell pellet using mortar and pestle in liquid nitrogen in the presence of Trizol (Invitrogen). The RNA was extracted using Trizol according to manufacturer's directions. 10-40 nt fraction was enriched using flashPAGE (Ambion). The adapters (Small RNA Oligo Only, Illumina) were ligated sequentially using T4 RNA Ligase (Promega); each step was followed by purification by denaturing PAGE. The library was reverse-transcribed using Superscript II (Invitrogen) and amplified using Phusion Hot Start DNA Polymerase (Finnzymes).
GEO Accession
GSM1309661