vanilla suppressive soil  to vanilla Fusarium wilt disease
Fungi-specific primers ITS1F (CTTGGTCATTTAGAGGAAGTAA) (Gardes and Bruns, 1993) and ITS2 (GCTGCGTTCTTCATCGATGC) (White et al., 1990) were used to target the ITS1 region (average length approximately 260 bp). Bacterium-biased primers 520F (AYTGGGYDTAAAGNG) and 802R (TACNVGGGTATCTAATCC) (Claesson et al., 2009) were used to amplify ~280-bp fragments of V4 hypervariable regions of the bacterial 16S rRNA gene.