GSM4629192: P1; Oryctolagus cuniculus; RNA-Seq
RNA was harvested using Trizol reagent A sequencing library was generated using the Illumina kit. The eukaryotic mRNA was enriched with magnetic beads with Oligo (dT); the mRNA was randomly interrupted by the addition of Fragmentation Buffer; the first cDNA strand was synthesized using random hexamers using mRNA as a template, and then The second cDNA strand was synthesized by adding buffer, dNTPs, RNase H and DNA polymerase I, and the cDNA was purified by AMPure XP beads; the purified double-stranded cDNA was subjected to end repair, A tail was added and the sequencing linker was ligated, and then AMPure XP beads were used. Fragment size selection; finally, a cDNA library was obtained by PCR enrichment. Initial quantification was performed using Qubit2.0, and the insert size of the library was detected using Agilent 2100. After passing the library test, different libraries are merged based on the amount of target data and sequenced using the Illumina HiSeq platform.
GEO Accession
GSM4629192