ATAC-Seq of Sus scrofa : muscle tissue of Large White at age 2 weeks
The pulverized tissue was suspended in 1mL ice-cold PBS, after removed supernatant, the pellet was suspended in 1 mL lysis buffer Isolation of 50,000 nuclei followed by one previous study with minor modifications (Buenrostro et al., 2015). The library was quantified and sequenced on an Illumina Hiseq Xten PE150 platform. The clean data was obtained after removing reads with adapter contamination or low quality.