GSM1537842: RNA-seq Luc Control replicate 1; Drosophila melanogaster; RNA-Seq
5x106 cells  were treated with 40µg/ml dsRNA corresponding to luciferase gene with boosts of dsRNA each day. On the third day the cells were harvested and total RNA was purified using TriReagent. 30ug of total RNA was enriched for mRNA using Poly(A)Purist MAG kit (Ambion) according to manufacturer’s specifications. Library was performed as described in (Kielpinski et al. 2013, Deep Sequencing Data Analysis). The 3' ligation adapter contains a 7'mer random barcode. 20 cycles of PCR was used to create the final library. See individual experiment
GEO Accession
GSM1537842