GSM3177100: run183.SB-15perc-ETOH_L006; Gallus gallus; RNA-Seq
RNA was extracted using a RNAqueous-4PCR Kit (Ambion/Applied Biosystems, Carlsbad, CA) cDNA libraries were constructed using the Illumina RNA Sequencing Sample Preparation kit. mRNA was purified from approximately 8 µg total purified RNA with poly-T oligo-attached magnetic beads. Double-stranded cDNA was synthesized using SuperScript II (Invitrogen, Carlsbad, CA) with random hexamer priming, purified with QIAquick PCR purification columns (Qiagen, Valencia, CA), ligated to Illumina adapters, and size-selected using electrophoresis to approximately 300bp.
GEO Accession
GSM3177100