16S rRNA gene sequencing of environmental sample: oilfield produced water
16S rRNA gene fragments from produced water (25 mL) were amplified using universal PCR primers 926F and 1392R. Nextera XT i7 and i5 adapters were added to purified amplicons using a second round of PCR. The final purified and normalized amplicons were sequenced using a MiSeq Reagent Kit v3. Raw forward and reverse sequence reads were merged in QIIME.