Illumina MiSeq sequencing; qiita_ptid_50:1713.McG.L2N1020
Within the larger 52-ha plot, three sub-plots (20 m X 20 m) were delineated on each of the four soil types for a total of 12 plots. Soil samples were collected from three sampling depths (0-2 cm, 2-10 cm, 10-20 cm) in each plot and fungal DNA has already been sequenced with Illumina (ITS region). Five soil cores from each sampling depth were composited from each plot for a total of 36 samples. All samples were extracted with the EMP protocol.
EMP V4 515f,806rbc protocol
barcode
CGCGTCAAACTA
center_name
CCME
center_project_name
mcguire_malaysialambir_soil
illumina_technology
MiSeq
linker
GT
pcr_primers
FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
primer
GTGTGCCAGCMGCCGCGGTAA
run_center
CCME
run_date
2013
run_prefix
McGuire_S0_L001
samp_size
.1,g
sample_center
McGuire Lab, New York NY
sequencing_meth
sequencing by synthesis
target_gene
16S rRNA
target_subfragment
V4