RNA-Seq of equus caballus: healthy adult female bone marrow: Sample healthy control horse C4
RNA was isolated from snap-frozen bone marrow samples following homogenization by pestle and QIAshredder (Qiagen, Valencia, CA) as directed by the RNeasy kit (Qiagen) including on-column DNase digestion. RNA was quantified with a Nanodrop (Thermo Fisher Scientific, Inc., Waltham, MA). RNA purity and integrity was assessed using a BioAnalyzer 2100 (Agilent, Santa Clara, CA), and all RNA samples had RIN values = 8.0. RNA-Seq library preparations were performed according to Illumina’s standard mRNA-seq kit protocol, and sequences of 88 bases in length were obtained on an Illumina HiSeq2000 by the Cornell University Institute of Biotechnology Genomics Facility, Ithaca, NY.
alignment_software
TopHat version 2.0.8