GSM4232480: mRNA seq from HepG2 negative conrol; Homo sapiens; RNA-Seq
Cell culture medium was removed, cells were washed with DPBS, TRIzol (Life Technologies) was added and cells were processed according to manufacturer's guidelines. Around 500 ng total RNA was used as an input for QuantSeq 3' mRNA-Seq Library Prep (Lexogen). The double stranded cDNA library is made by first strand synthesis with a oligo-dT primer containing the Illumina-specific Read 2 linker and second strand synthesis with random primer containing the Illumina-specific Read 1 linker sequence. Library amplification introduces a unique i7 index for each sample and sequences required for cluster generation.
GEO Accession
GSM4232480