GSM2153542: C1-28-F9_GCTACGCT-ACTGCATA_L002_R1_all; Homo sapiens; RNA-Seq
Fluidigm C1 was used to capture single cell for mRNA sequencing.   Catalog # 100-5760, which is microfluidic IFCs designed to capture cells from 10 to 17 µm.  Since each Fluidigm C1 chip could maximum capture 96 cells,  we sequentially numbered the chips we used during the experiments.  So, the numbers in  the Characteristics "chip" field column indicate the cell was captured from which chip.  This information was provided because it could help the estimation of the systematic effects coming from the chips. RNA was extracted after single cell capture using C1 single cell auto prep system, with manufacturer protocol. RNA libraries were prepared for sequencing using with Illumina NEXTERA XT kit Single cell RNA-Seq
GEO Accession
GSM2153542