Sequencing of the sample 14069 and alignment to H37Rv reference
Mycobacterium tuberculosis isolate was cultured once Middlebrook 7H10 agar. Genomic DNA was extracted using CTAB/NaCl method. The amount of genomic DNA was checked by Quant-iT™ PicoGreen® dsDNA Assay Kit (Life Technologies). Genomic DNA was fragmented by sonication using a TruSeq gDNA Library automate (Illumina). Genomic DNA quality was estimated by High Throughput Quality Check for Massively Parallel Sequencing Library and fragments were multiplexed by 24 for Paired-ends 250 bp sequencing using MiSeq 250 System (Illumina).