sRNA seq of Beauveria bassiana mycelia recovered from silkworm cadavers
Beauveria bassiana mycelia were harvested from the silkworm cadavers to extract total RNA. Total RNA was used to construct sRNA library as the following step. (1) Filter sRNA: separate 18~30nt RNA segment by PAGE gel. (2) 3' adaptor ligation. (3) Reverse primer annealing. (4) 5' adaptor ligation. (5) Strand cDNA synthesis. (6) PCR amplification. (7) Library fragment selection. (8) Library quantitative and pooling cyclization.