GSM1534563: PER2 siRNA 3; Homo sapiens; RNA-Seq
RNA was purified using RNA STAT-60 (AMS Bio) according to manufacturer's instructions and treated using Amplification Grade DNase I (Invitrogen) followed by ethanol precipitation and clean-up.  RNA quality was examined using the Agilent Bioanalyser 2100 and all samples had a RIN ≥8.9 Library preparation was performed using Illumina TruSeq Stranded mRNA sample prep kit according to manufacturer's instructions.  The libraries were validated on the Agilent BioAnalyser 2100 to check the size distribution of the libraries and on the Qubit High Sensitivity to check the concentration of the samples.  Samples were pooled and pools were loaded at a concentration of 8pM onto 2 lanes of an Illumina HiSeq2000 flow cell v3.
GEO Accession
GSM1534563