Expression analysis of RNA from light-pulsed of Neurospora crassa
RNA was harvested from Neurospora crassa cultures grown in the light for 24 hours, dark for 24 hours, given a light pulse for one hour and then harvested. RNA was extracted using Qiagen's plant RNA extraction kit.  Library was prepared using the SOLiD Total RNA-seq kit. Two rounds of rRNA depletion was performed before the library prep using Invitrogen's (now Life Technologies) Ribominus kit. CH03 and CH09 are biological replicates.