GSM1640451: Sample_2172: C57BL/6J DEHP300mg/kg/day; Mus musculus; MBD-Seq
The mouse vas deferens and epididymis is dissected out, put onto a Petri dish with a droplet of Phosphate-Buffered Saline (PBS), and scored with a razor blade into small pieces to allow sperm to diffuse into the medium. The latter is then transferred into an Eppendorf tube and the fragments were allowed to sediment during 30 min at 37°C. The supernatant is carefully transferred to another tube and this procedure is repeated 3 times. The final supernatant is carefully removed and centrifuged at 6’000 x g for 3 minutes to pellet the sperm. DNA extraction from sperm cells is performed with the QIAamp DNA Micro kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol. DNA was fragmented into ~200bp with Covaris S2, subjected to MethylCap kit (Diagenode, Seraing, Belgium) before libraries amplification for multiplex sequencing following an adapted version of ChIP-seq protocol by NXT-DX.
GEO Accession
GSM1640451