epPCR mutated pooled ANO1 plasmid library
Library construction: epPCR was used to mutate the coding sequence of the synthetic human gene ANO1 (TMEM16A).  The sequence was cloned into an E. coli plasmid (pcDNA5-HA, Life Technologies), isolated, and pooled for sequencing.  Experimental design: pooled 6,000 epPCR mutated, synthetic ANO1 sequences.  Paired-end, Illlumina, sequencing on the pooled library