GSM4629864: KARPAS_D0_A; Homo sapiens; OTHER
Genomic DNA was extracted using Phenol/Chloroform protocol. Two round of PCR reaction was used for library constrution. For the first PCR, primers that amplify the lentiCRISPR sgRNA were used for 18 cycles and for each sample, 13 separate 100ul reactions with 10ug genomic DNA was used. The amplicons were combined and second round PCR was performed to attach Illumina adaptors for 18 cycles. The reaction was performed with 5ul of first PCR in 100ul reaction volume. The resulting amplicons were gel extracted and quantified and mixed and sequenced using one end - pairing  Illumina Next Generation Sequencing following the manufacturer's protocols. Next Generation 500 Illumina Sequencing
GEO Accession
GSM4629864