ChIP-Seq adults HR H3K9Ac
For ChIPseq library construction the ChIP sample preparation kit (Illumina Inc., USA) was used according to the manufacturer's protocol except for the indexed adapters. Those adapters came from the Truseq DNA sample preparation kit (Illumina Inc., USA). Briefly, 30 ng of DNA per condition was blunt ended and adenylated on its 3' ends. Illumina's indexed adapters were ligated to both ends of the adenylated DNA. Ligated DNA was size separated by electrophoresis through a 2 % agarose gel and a size selection was performed at 400 bp. Once extracted from the agarose, size selected DNA was enriched by 18 cycles of PCR for 10s at 98°C, 30s at 60°C and 30s at 72°C using Illumina's proprietary primers and Phusion DNA polymerase. Each library was verified using a DNA 1000 Chip on a Bioanalyzer 2100, quantified by real time PCR with the KAPA Library Quantification Kit for Illumina Sequencing Platforms (Kapa Biosystems Ltd, SA) and then diluted to 10 nM.