GSM3177083: MR_4; Danio rerio; RNA-Seq
RNA was extracted with PicoPure™ RNA Isolation Kit Index-tagged cDNA libraries were constructed, in two batches, from total RNA (1 ng) with the TruSeq RNA sample preparation v2 kit (Illumina), after amplification with the NuGen OvationSC RNA-Seq System. Quality, quantity, and size distribution of the Illumina libraries were determined using the DNA-1000 kit (Agilent Bioanalyzer). RNASeq. Libraries were sequenced (single-end mode and length of 60-61 bp) on the Illumina HiSeq 2500 system using the standard RNA sequencing protocol in the TruSeq SBS kit v5.
GEO Accession
GSM3177083