GSM1537834: RNA-seq replicate 2 (Control for iCLIP rep2); Drosophila melanogaster; RNA-Seq
Total RNA was isolated from 5x107 S2 cells using the standard TRI Reagent protocol (Sigma Aldrich). Poly(A) RNA was enriched from 100ug total RNA using Poly(A)Purist MAG kit (Ambion) according to manufacturer’s specifications. Poly(A) RNA was fragmented using MgCl2. The rest of the library preparation was performed as described in (Konig et al, 2011) with PCR amplification using 17 cycles. See individual experiment
GEO Accession
GSM1537834