amplicon: CRISPR1 array, Pfu CasOE JFW02 strain with plasmid pYS3-Pgdh
DNA was isolated from cultured Pyrococcus furiosus that had been transformed with plasmid pYS3-Pgdh.  A region of the CRISPR1 array corresponding to newly acquired spacers was amplified from total DNA by PCR.  Illumina adapters were added through additional rounds of PCR.