S36h GPQ-52 L007
1µg RNA was processed for library preparation using TruSeq RNA Sample Prep kit (Illumina). Transcripts were purified and fragmented by zinc breaking using the mRNA-SEQ kit (Illumina). RT yielding double-stranded cDNA fragments was performed with random primers and RNA was removed using RNaseH. After ends-reparation and purification, fragments were ligated with Illumina adapters from the kit. The first cDNA strand was then removed and libraries were amplified by PCR for 15 cycles. Fragments were purified on agarose gel to recover fragments with inserts in the range of 160-240 base pairs. All libraries were sequenced in 2 independent sequencing runs on the Illumina HiSeq2000 using TruSeq SBS Kit v3.