SOLiD RNA-SEQ basespace sequencing library from placental/ovarian tissue of individual Poeciliopsis prolifica female
RNA was isolated from ovarian/placental tissue of a single Poeciliopsis prolifica female using the Qiagen RNeasy kit. All developing embryos, their belly sacs, and fertilized embryos were dissected out of tissues before RNA isolation. Ribosomal RNA was removed from RNA samples using Epicentre Ribo-Zero rRNA Removal kit for individuals #6 and #7. The Invitrogen Ribo-Minus kit was used for individual #3 only as it was judged to be less effective than Epicentre kit. Sequencing libraries from resulting RNA products were constructed using standard SOLiD cDNA library construction protocols. All barcodes were removed post-sequencing using the program Cutadapt.