GSM2231268: Detroit562_cellular; Homo sapiens; miRNA-Seq
Total RNA was extracted from each exosome pellet using the miRNeasy Micro kit (Qiagen, Valencia, CA) according to the manufacturer’s suggested protocol; total RNA was also extracted from the pooled cells for each respective cell-line the miRNeasy Micro Kit (Qiagen), with 7.5 x105 cells in each reaction. The NEBNext small RNA sample library preparation kit (New England BioLabs, Ipswich, MA) was used with 20 ng to 1 µg of total RNA in a 6-µL solution as input, following the manufacturer’s protocol with modification of library size selection
GEO Accession
GSM2231268