GSM4232921: ATAC_Ep_LD-rep1; Arabidopsis thaliana; ATAC-seq
ATAC-seq: Prepare 50,000 fresh isolated beads conjured nuclei for the consequent ATAC-Seq tagmentation. Convert the buffer from NPBt into tagmentation reaction (Vazyme TD501) using magnet rack. Incubate the beads conjured nuclei 37℃ for 30 min. Extract DNA by a CTAB-chloroform based method and finally store the DNA water solution at -20℃. Follow the Vazyme TD501 manual to set up ATAC-Seq library. 12 cycles are applied to the PCR step. All these steps above, from material collection until DNA extraction must be executive continuously to ensure the chromatin fresh. RNA-seq: Total nuclei RNA was extracted from about 100,000 isolated nuclei using the RNeasy Micro Kit (Qiagen, 74004). cDNA was amplified using the Single Cell Full Length mRNA Amplication Kit (Vazyme, N712) according to the user manual. The RNA-seq library of each sample was prepared using TruePrep DNA Library Prep Kit V2 for Illumina® (Vazyme, TD501). The library was purified by the VAHTS DNA Clean Beads (Vazyme, N411) and quantified using Qubit and Agilent 2100 before sequencing by the Illumina HiSeq XTen.
GEO Accession
GSM4232921