GSM2154988: ChIP-Seq GR wild type plus ICI; Homo sapiens; ChIP-Seq
For ChIP-seq, some samples were fixed for 10 minutes with 1% formaldehyde. Fixation was stopped by adding Glycine (0.125M). Chromatin DNA was sheared to 200–500 bp average in size by sonication. Soluble sheared chromatin was immunoprecipitated with specific antibodies or streptavidin beads overnight at 4°C. After washing and elution, the protein–DNA complex was decross-linked by heating at 65°C overnight. Immunoprecipitated DNA was purified by using iPure Diagenode kit. the libraries were constructed following Illumina's Chip-Seq Sample prep kit.
GEO Accession
GSM2154988