GSM3258334: jazD_rep1; Arabidopsis thaliana; RNA-Seq
For each replicate sample, approximately 20 plants (whole rosette, excluding roots) per genotype were pooled and immediately frozen in liquid nitrogen and stored at -80 ºC until processing. Wild-type Col-0 and mutant plants were grown in the same tray to minimize tray-to-tray variation. Three independent samples (biological replicates) were sequenced per genotype. Total RNA was isolated using a Qiagen RNeasy kit with Qiagen on-column DNase treatment to remove genomic DNA. RNA integrity was assessed with a 2100 Bioanalyzer (Agilent Bioanalyzer) and all samples had an integrity score of at least 7.0. Barcoded sequencing libraries were prepared using the Illumina RNAseq kit according to the manufacturer's instructions. Normalized libraries were sequenced on the Ilumina HiSeq 2000 platform with multiplexing of nine libraries per lane.
GEO Accession
GSM3258334