GSM2842620: GFPctrl_KSL_HSC; Mus musculus; ChIP-Seq
Lysates were clarified from sonicated nuclei following crosslinking with 1% formaldehyde, and histone-DNA complexes were isolated with antibody. For detailed protocol see Wilson et al., Cell Stem Cell. 2010 Oct 8;7(4):532-44, modified in that the sonication was 4 cycles (30 secs on, 30 secs off). According to Illumina TruSeq DNA Sample Prep Kit
GEO Accession
GSM2842620