GSM4681765: Breast cancer cells - MCF7 CL; Homo sapiens; RNA-Seq
After treatment, cells were detached using phenol-red free TrypLE (Gibco) and collected in 1X DPBS (Gibco). After one wash in 1X DPBS, cells were resuspended in 2% BSA-DPBS at a concentration of 10000 cells/ml. Following incubation with TruStain Fcx blocking solution (BioLegend), Totalseq A Hashtag antibodies were added per treatment group. Cells were washed 3 times with 1 ml 2%BSA-DPBS and resuspended to a volume of ~10000 cells/ml. Equal volumes of each treatment group were pooled and cell pool was assessed for cell concentration and viability. Single-cell cDNA and HTO libraries were generated using Chromium Single Cell 3′ version 3 and 3.1 reagents (10x Genomics and Biolegend) per manufacturer's protocols. Single-end sequencing of libraries was performed per manufacturer's protocol on a Novaseq 6000 (Illumina) sequencer with HTO libraries constituting 5% of the sample.
GEO Accession
GSM4681765