aneuploid tumor 5
DNA was extracted from a representative area of a 50 ?m section using proteinase K digestion. Targeted next generation sequencing was performed with a capture assay, termed OncoVar, which was designed to sequence coding exons of 563 cancer related genes (Boikos SA et al. JAMA Oncol 2016). The resulting paired-end libraries were sequenced on Illumina NextSeq 500 sequencers.