GSM4506506: DRG_1d_SCI_3; Mus musculus; RNA-Seq
L4 DRG were dissected and homogenized in 300μl of lysis buffer on ice and RNA purified using the PureLink RNA Mini kit. RNA quality was assessed using an Agilent Bioanalyzer (RIN > 8). Samples were subjected to DNase treatment. rRNA depletion was achieved with the Ribo-Zero rRNA removal kit. Library preparation was performed from purified RNA using the SMARTer kit for Illumina sequencing (Clontech) per manufacturer's instructions.
GEO Accession
GSM4506506