GSM2341239: RAL_42-axenic; Drosophila melanogaster; RNA-Seq
On dry ice (-80°C), replicate vials were pooled and male flies sorted into chilled sterile microcentrifuge tubes containing 110 ml of 1.4 mm lysing matrix (MP Biomedicals), 500 ml buffer RLT (Qiagen), and 5 ml b-mercaptoethanol. Flies were immediately homogenised at 4.0 m/s for 30 s on a Fastprep-24 (MP Biomedicals) and frozen at -80°C. RNA was isolated using the RNeasy mini kit (Qiagen) TruSeq RNA Sample Prep Kit v2 – Set A (Illumina RS-122-2001). The samples were sequenced (50 bp single end reads) on the Illumina HiSeq 2500 platform in multiplexed pools of 6 samples per lane.
GEO Accession
GSM2341239