GSM2424664: Sample 6_DGCR8_48h_4oht_rep1; Mus musculus; RNA-Seq
Samples 1-35: total RNA was extracted from mESCs with the Absolutely RNA Microprep Kit (Stratagene). RNA was subjected to ribosomal RNA depletion using Ribozero removal kit (Illumina) followed by library construction using ScriptSeq V2 library preparation kit (Illumina) Samples 36-59 Genomic DNA was extracted using Phenol/Chloroform followed by  EtOH precipitation in the presence of sodium acetate and molecular biology grade glycogen Samples 1-35: ScriptSeq v2 RNA-Seq Library Preparation Kit. Samples were treated with a ribosomal depletion kit Samples 36-41:Illumina ChIP-Seq prep kit starting from 5ng purified ChIP DNA or respective input. Samples 42-59 :ChIP-seq library preparation kit from NEB. Starting with 10ng purified ChIP DNA or resepective input.
GEO Accession
GSM2424664