Transcriptome dynamics of the floral buds from male and female plants of Coccinia grandis
The paired-end cDNA sequencing library from the QC passed sample was prepared using illumina TruSeq stranded mRNA Library Preparation Kit and as per its described protocol. Briefly, mRNA was enriched from total RNA followed by fragmentation. The fragmented mRNA was converted into first-strand cDNA, followed by second-strand generation, A-tailing, adapter ligation and finally ended by limited number of PCR amplification of the adaptor-ligated libraries. Library quantification and qualification was performed using DNA High Sensitivity Assay Kit. The sample was loaded on to a Illumina NextSeq 500 platform for sequencing