RNA-sequencing DE Spd (humerus)
RNA-sequencing DE Spd (humerus)
Heterozygous matings of Splotch-delayed mice were harvested at E14.5 (TS23) Splotch-delayed homozogous mutant and control (homozygous wild-type and heterozygote) embryos were dissected and Humerus forelimb tissue was dissected at embronic day 14.5 (Theiler Stage 23) RNA was extracted following manufacturers guidlines ( SV. Total RNA Isolation system, Promega, UK) The DNase-treated RNA (3ug) was used to prepare RNA-Seq libraries with the TruSeq RNA Sample Prep kit. A total of six cDNA libraries were constructed, representing triplicate biological replicates for each group.
Experimental Factor: genotype
Splotch-delayed (Pax3Spd/Spd)