Illumina HiSeq 2000 sequencing; Capture sequencing analysis of Miwi2-asEGFP transgene
Capture sequencing analysis of Miwi2-asEGFP transgene
Mice were grown under the following condition: temperature 23.5 degree centigrade +/-2 degree , humidity  50 +/-10 % and 12 hour illumination (light period 8:00-20:00) The mouse tail was treated with Protease K and RNase A.  The equal amount of Phenol/Chloroform solution was added. After centrifugation, the sup was harvested and then the equal amount of isopropanol was addicted.  The DNA pellet was centrifugated and washed with 70% ethanol.  The genomic DNAs were dried up at 55 degree centigrade.  The DNAs were dilluted with TE. The extracted DNAs were fragmented to a length of 150 -200 nt by Covaris.  These DNAs were amplified and concentrated with SureSelect XT Target Enrichment System (Agilent technologies) according to the manufacture's protocol. The bait RNAs corresponding to Miwi2 Promoter sequence were synthesized by Hokkaido System Science.
Experimental Factor: genotype
Miwi2-asEGFP#6
LIBRARY_STRAND
not applicable