RNA-Seq of Mus musculus adult female MEP
MEP (CD34- FcgR- cKit+ Sca1- Lineage-) cells were isolated from adult female miR-22KO mice. Total RNA was isolated using Qiagen miRNeasy Mini Kit. Reverse transcription and cDNA amplification were performed with the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech). Librarieswere constructed using the Illumina Nextera XT kit. Sequencing performed on Illumina HiSeq 2500, 2x150bp.