Liming plot 1 - rainy season
The 16S rRNA gene library preparation for sequencing was performed by Macrogen. PCR reactions were carried out to target V4 hypervariable region of the 16S rRNA gene using primers 515F (GTGYCAGCMGCCGCGGTAA) and 806R (GGACTACNVGGGTWTCTAAT) (Apprill et al. 2015; Parada et al. 2016). The protocols for PCR reactions followed the indications of the Earth Microbiome Project (http://www.earthmicrobiome.org/protocols-and-standards/). The amplicon sequencing was performed in a 2  300 bp paired-end Illumina MiSeq Next-Generation Sequencer, using Nextera XT Index Kit V2 and Herculase II Fusion DNA Plymerase enzyme.