RNAseq_marine_metatranscriptome_0.4-20um_summer_surface
RNA extraction, Dnase treatment, rRNA depletion (RiboZero - bacteria kit),  purification, tRNA removal (Agencourt RNAClean XP kit - Beckman Coulter genomics), sonication, purification, first strand cDNA synthesis (N6 randomized primers), adapter ligation at both ends (3' adapter included barcodes), PCR amplification (17 cycles with high fidelity polimerase),  cDNA purification, equimolar pooling of barcoded samples, size selection (300-600bp) using preparative gel