GSM2844608: Clostridium perfringens infected rep2; Mus musculus; RNA-Seq
Total RNA from mouse muscle tissue was harvested using Trizol reagent.  The total RNA was further purified through the Qiagen RNeasy mini columns with the additional on column DNase step RNA libraries were prepared according to the Life Technologies SOLiD Total RNA-Seq Kit.  Briefly 5ug of total RNA was depleted of both eukayotic and bacterial rRNA, using an 8:2 ratio of eukaote:bacteria probe mix.  Following enzymatic shearing, 100ng of ribo-depleted  RNA was used to make whole transcriptome libraires according to the SOLiD Total RNA-Seq protocol; Pub 4452437 RevB
GEO Accession
GSM2844608