NextSeq 500 paired end sequencing; RNA-seq analysis of memory phenotype and naive CD4 T cells in the steady state
RNA-seq analysis of memory phenotype and naive CD4 T cells in the steady state
CD4+CD25-CD44lowCD62L+ cells were isolated from Egr2/3 DKO or Egr2 Kin mice by FACS RNA was isolated and purified using TRIzol reagent (Life Technologies) according to the manufacturer's instructions RNA from T cells was processed for directional mRNA-Seq library construction using the KAPA mRNA HyperPrep Kit (Kapa Biosystems) according to the manufacturer's protocol
Experimental Factor: genotype
Egr2_Kin
Experimental Factor: cell type
CD62L