GSM2231423: DNA_KO; Mus musculus; Bisulfite-Seq
Genomic DNA samples were purified from subconfluent MEFs by phenol extraction and ethanol precipitation. Bisulfite-converted genomic DNA libraries were prepared according to the previously described methods. Briefly, genomic DNA was digested with MspI (New England Biolabs), followed by end-repair and addition of 3′ A overhangs. Methylated adaptors (Illumina) with a 3′ T overhang were ligated to the A tailed DNA fragments. For reduced representation, 40 to 220 bp (pre-adaptor-ligation size) fragments were excised from 2% TAE agarose gels and bisulfite-converted with EZ DNA methylation Gold kit (Zymo Research). Bisulfite converted libraries were amplified by PCR.
GEO Accession
GSM2231423