Microbial communities from Hydrothermal Plumes at ELSC
Waters were filtered through a 0.8 micron filter in situ and preserved on RNALater immediately upon reaching the deck of the research vessel. DNA was extracted using methods described in Dick et al. 2010, Environmental Microbiology. The 16S rRNA gene was amplified using near universal primers targeting the V4 region 515F/806R. See Anatharaman et al. 2013, PNAS, for more information on amplification details.