Euprymna scolopes Hemocyte transcriptome
Hemocytes were extracted from 11 squid.  RNA was extracted from these cells using TRIzol.  rRNA was reduced using a Ribominus kit (Invitrogen).  500ng of remaining RNA was used to make a 454 cDNA sequencing library according to the standard sequencing protocol (Roche).  This library was then sequenced on one-half of a picotitre plate on a 454 GS-FLX sequencing machine using Titanium chemistry.