GSM4506543: FACS_SNI_3D_2c; Mus musculus; RNA-Seq
1 and 3dpi L4 DRG were dissected, dissociated with papain and collagenase, and passed through a 70 um filter. Cells were FACS sorted for YFP-positive cells into Clontech lysis buffer with 5% RiboLock Rnase Inhibitor (Thermo Fisher). Library prep was performed with ~100 cells collected by flow cytometry in 10 ul of Clontech's reaction buffer using the SMARTer Ultra Low RNA kit for Illumina Sequencing (Clontech) per manufacturer's protocol. Three technical replicates of 100 cells each (A-C) were obtained for each biological replicate.
GEO Accession
GSM4506543