GSM3759280: myc_3; Drosophila melanogaster; RNA-Seq
The cells were dissociated and the GFP-positive cells were sorted by the Cytometry and Cell Sorting Core at NYU Langone Medical Center using a Sony SY3200 cell sorter per the protocol described in (de la Cruz and Edgar 2008). The sorted cells represent GFP-positive control cells from the dpp domain (referred to as “GFP” samples), GFP-positive cells from the dpp domain that had ectopic JAK/STAT signaling as a result of mis-expressing Hop (referred to as “Hop” samples) or GFP-positive cells from the dpp domain that had elevated Myc levels as a result of mis-expressing Myc (referred to as “Myc” samples). RNA was isolated from the sorted cells using TRIzol reagent (Ambion) and then purified using RNeasy Mini Kit (Qiagen) per the manufacturer's instructions. The RNA sequencing was performed by the Genome Technology Center at the NYU Langone Medical Center using Illumina HiSeq2500 Paired-End 50 Cycle Flow Cell.
GEO Accession
GSM3759280