WGS of Escherichia coli strain isolated from an OLP biopsy
To generate libraries, 400 ng gDNA was fragmented into approximately 550 bp using the M220 Focused-ultrasonicatorTM (Covaris, Brighton, UK), and the fragmented DNA was quantified using the DNA 7500 kit (Agilent, Palo Alto, USA) and the Bioanalyzer 2100 (Agilent). Libraries were then constructed using the TruSeq DNA Library LT kit (Illumina, San Diego, USA) according to the manufactureâs protocol. The whole-genome sequencing was performed on an Illumina MiSeq platform with 2 x 300 bp paired-end reads using the Illumina MiSeq Reagent Kit v3 600 cycles kit (Illumina).