polyethylene glycol 6000, mixed sample of 0,6,12,18,24hrs
Tall fescue seedlings were grown (from seed) with 16/8 h day/night cycles at 25/20 °C (65% relative humidity) in a plant incubator. After 50 d, healthy seedlings (without a tiller) were selected and placed in 20% (W/V; -0.53408 MPa) polyethylene glycol 6000 (PEG 6000) solution and subsequently removed after 0, 6, 12, 18, or 24 h, respectively.