GSM1538095: MC32; Homo sapiens; RNA-Seq
RNA was extracted from fresh frozen tissue using the Roche High Pure FFPE kit (Roche Diagnostics). After Ribogreen quantification and quality control via Agilent BioAnalyzer, 0.5 to 1 ug of total RNA underwent stranded TruSeq library preparation according to the manufacturer's instructions (Illumina), with 6 cycles of PCR. Samples were barcoded and run on a HiSeq 2000 in a 50bp/50bp paired-end run, using the TruSeq SBS Kit v3 (Illumina). An average of 69 million paired reads were generated per sample. Ribosomal reads represented at most 0.83% of total reads, while mRNA bases accounted for an average of 61% of reads.
GEO Accession
GSM1538095