GSM4633420: BioGRO_xrn1delta; Saccharomyces cerevisiae; RNA-Seq
RNA extraction: After run-on labelling, cells were kept on ice for 5 min and harvested by centrifugation. RNA extraction was done using the 'MasterPure Yeast RNA Purification Kit' (Epicentre) following the manufacturer's instructions. Once extracted, genomic DNA was removed by digesting with 2 uL of RNase-free DNase I (Roche) for 30 min at 37 ºC. Purified RNA was resuspended in 32 uL of water and was spectrophotometrically quantified. Biotinilated RNA purification: Labelled RNA was subjected to 1 round of affinity purification with streptavidin paramagnetic beads Dynabeads M-280 Streptavidin (Invitrogen). Purified labelled RNA was end-repaired with PNK prior to library preparation with the NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible)
GEO Accession
GSM4633420