GSM1905388: P7_Id4; Mus musculus; RNA-Seq
Cells were isolated from C57Bl/6 mice at postnatal day 0,7,12,14 (labeled P0 to P14 respectively). These cells were further selected for specific markers (Oct4, Thy1, Id4 or Kit). Cell populations labeled with GFP were selected for GFP via FACS. FACS analysis involved isolations from P0 Vasa-GFP+ (JAX Lab, 006954), P0 and P7 Oct4-GFP+ (JAX Lab, 008214); and P7Id4-GFP+ (J. Oatley). Cells were sorted using FACSCalibur (BD Biosciences, SanJose, CA). The percentage of live cells was >95%, by exclusion of propidium iodide. Isolation of either the THY1+ or c-KIT+ stem cell subtypes from C57Bl/6 mice was carried out with a magnetic cell-sorting (MACS) separator (MiltenyiBiotec, Bergisch-Gladbach, Germany) using anti-CD117 (KIT) antibody or anti-CD90.2 (THY1) (MiltenyiBiotec). Total RNA was extracted using  Qiagen AllPrep DNA/RNA/Protein mini kit (Cat # 80004), ribosomal RNA was depleted using RiboZero kit. Directional RNA library was prepared according to Illumina's standard protocol. Detailed experimental procedures and bioinformatics analysis can be found in the supplemental method section of the paper.
GEO Accession
GSM1905388