GSM4568476: A549_siControl replicate1; Homo sapiens; RNA-Seq
Total RNA was isolated using a NucleoSpin RNA kit (Macherey Nagel, Germany). The polyA fraction was purified from 500ng of total RNA, followed by fragmentation and generation of double-stranded cDNA, Agencourt Ampure XP beads cleanup (Beckman Coulter, USA), end repair, A base addition, adapter ligation and PCR amplification. Libraries were quantified by Qubit (Thermo fisher scientific, USA) and TapeStation (Agilent, USA).
GEO Accession
GSM4568476