GSM2143383: TERAT_KI_2 (II); Mus musculus; RNA-Seq
miRVana RNA isolation Kit form Thermo Fisher Scientific 0.8 µg of total RNA sample (0.5 µg for sample iPS_KI_C1) was extracted from the cells. RNA Integrity Numbers were in the range 7.-10.0 when assayed on an Agilent 2100 Bioanalyzer. PolyA+ fraction was purified and randomly fragmented, converted to double stranded cDNA and processed through subsequent enzymatic treatments of end-repair, dA-tailing, and ligation to adapters as in Illumina's "TruSeq Stranded mRNA Sample Preparation Part # 15031047 Rev. D" kit (this kit incorporates dUTP during 2nd strand cDNA synthesis, which implies that only the cDNA strand generated during 1st strand synthesis is eventually sequenced). Adapter-ligated library was completed by PCR with Illumina PE primers (8-11 cycles). The resulting purified cDNA library was applied to an Illumina flow cell for cluster generation and sequenced on an Illumina HiSeq2000 sequencer by following manufacturer's protocols. Strand-specific RNA-Seq (dUTP)
GEO Accession
GSM2143383