GSM2586812: WT-derived culture; Mus musculus; RNA-Seq
Total RNA was extracted from cells using TRIzol reagent (Life Technologies) and the quality was assessed with Agilent 2100 Bioanalyzer picochips. mRNA enrichment with Oligo dT, mRNA fragmentation, reverse transcription, adaptor ligation and PCR were performed using the Illumina TruseqTM RNA Sample Prep Kit. RNA libraries were prepared for sequencing using standard Illumina protocols with Illumina TruseqTM RNA sample prep Kit.
GEO Accession
GSM2586812