Calanus finmarchicus Transcriptome-adult females (CVI)-7415
Double-stranded cDNA libraries were prepared from total RNA extracted using the TruSeq RNA sample preparation kit (Illumina catalog # RS-122- 2001) following manufacturer’s instructions. Briefly, RNA samples were first purified with two oligo-dT selection (poly(A) enrichment using oligodT beds), and then fragmented and reverse transcribed into double-stranded complementary cDNA. The resulting six cDNA libraries were prepared with a 350 bp insert and primed using random hexamers. Each sample was tagged with an indexed adapter.