Small RNAs-derived cDNA library of liver of single male rat (216 days) born to HF-fed dam and fed HFdiet at 4.5 months
Total RNAs were purified using Qiazol lysis reagent (Qiagen). RNAs were size-fractionated on a 17% Tris-Borate-EDTA urea (8M) polyacrylamide  gel. Small RNAs (18-36nt) were recovered from gel slices and used to  build a cDNA library following an Illumina-like protocol. CDNA Library was constructed with the 3' RA3 Truseq adaptor and the tagging index I21.