RNA-Seq of mutant fish
Total RNA was isolated from the 15 dpf (days post-fertilization) bmpr2b mutant larval fishes using RNAiso Plus Reagent (TaKaRa, China) according to the manufacturers protocol. After RNA quality and quantity control, the transcriptome equencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) following manufacturers recommendations and index codes were added to attribute sequences to each sample.