GSM2769711: Day8 WT Tfh,  Ezh2 ChIP-Seq; Mus musculus; ChIP-Seq
CD4+ follicular helper T cells were sort-purified on day 8 post-infection. Tcf1- or Ezh2-deficient naive CD4+ T cells were sorted from uninfected naive mice. The cells were then fixed and sonicated to generate chromatin fragments, followed by immunoprecipitation with antibodies against Tcf1 or Ezh2, which were then properly washed and immunoprecipitated DNA extracted. The DNA segments from ChIP were end-repaired and ligated to indexed Illumina adaptors, followed by amplification by PCR with a low number of cells (18-21 cycles). The resulting libraries were seqeunced with Illumina Hiseq-2000 platform.
GEO Accession
GSM2769711