GSM2422812: Sample_7752_Sham_filtered_air_exposure; Mus musculus; RNA-Seq
RNA was extracted from frozen heart tissue using the Qiagen miRNeasy kit, following the manufacturer's recommendations. Quality and quantity were assessed using an Agilent 2100 Bioanalyzer, and NanoDrop 8000 spectrophotometer, respectively. RNA samples were depleted of ribosomal RNA using the Low Input RiboMinus Eukaryotic System v2 kit (Thermo Fisher Scientific). The cDNA library was then created with the ribosomal depleted RNA using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific). This included fragmentation via RNase III, hybridizing and ligating the RNA, performing reverse transcription, and amplification.  Samples were labeled with the Ion Xpress RNA-Seq Barcode 1-16 Kit (Thermo Fisher Scientific) to enable multiplexing 4 samples per run.  Templating, loading and sequencing were carried out using the Ion PI Template OT2 200 Kit v2, the Ion PI Chip Kit v2, and the Ion PI Sequencing 200 Kit v2 respectively (all from Thermo Fisher Scientific).
GEO Accession
GSM2422812