Paired end reads used to assemble Strongylocentrotus purpuratus de novo transcriptome
Embryos were collected and put in Trizol. RNA was extracted and then cleaned with a Qiagen RNeasy Micro column with on-column DNA digestion. The sequencing libraries were prepared with Illumina reagents, (mRNA-Seq Sample Prep Kit) with the maximum recommended RNA input. The protocol was followed exactly with the addition of a gel selection step, (agarose gel) prior to PCR amplification.