GSM4194478: ChIP-seq of GFP-Rix1 in wild-type cells; Schizosaccharomyces pombe; ChIP-Seq
Fixed cells were lysed with glass-beads, DNA sheared by sonication to 400-600bp fragments and immunoprecipitated with GFP antibody (Abcam, ab290). Immunoprecipitated DNA was recovered by incubation with protein A/G slurry and reversed-crosslinked at 65˚C. NEBNext Ultra II DNA Library Prep kit (NEB, E7645S) was used according to manufacturer's recommendation. Libraries were analyzed using an Agilent 2100 BioAnalyzer (Agilent) and sequenced on the Illumina MiSeq platform.
GEO Accession
GSM4194478