BB5
DNA was extracted using the PowerSoil DNA Isolation kit (MO BIO Laboratories, Carlsbad, CA, USA) with the HMP modifications to the manufacturers protocol. DNA quality and yield were evaluated via agarose gel and Qubit fluorometer (Life Technologies Corporation, Carlsbad, CA, USA). The 16S rRNA gene libraries were generated by the Center for Metagenomics and Microbiome Research at Baylor College of Medicine, using the V3-V5 (357F/926R) primer in accordance with standard HMP protocols.  The 16S rRNA libraries were sequenced by the Human Genome Sequencing Center (HGSC) at Baylor College of Medicine using a Roche 454 GS FLX+ instrument (Roche, Indianapolis, IN) operated with titanium chemistry.