GSM1419176: Infected cecum repeat 2; Gallus gallus; miRNA-Seq
Cecum was removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent The smaller RNA fraction (<100KDa) was further fractioned on a 15% tris-borate-EDTA (TBE) urea polyacrylamide gel and the 18-38 nt fraction was obtained. Subsequently, 5′ and 3′ RNA adapters were ligated to the small RNA by T4 RNA ligase (Promega), followed by reverse transcription and PCR product purification. Purified products were quantified on the Qubit® 2.0 fluorometer (Invitrogen) using QubitTM dsDNA HS  array kit(Invitrogen), then were diluted to 2 nM to finish cluster generation
GEO Accession
GSM1419176