GSM3326039: Kwon201408_XenopusHybrid_LELS1; Xenopus laevis; RNA-Seq
To isolate RNA, embryos at stage 9 were homogenized mechanically in TRIzol® (Thermo Fisher Scientific, Waltham, MA) using up to a 30-gauge needle and processed according to manufacturer instructions. After resuspension in nuclease-free H2O, RNAs were cleaned using a RNeasy kit (Qiagen Inc.) according to manufacturer instructions. Libraries were prepared using manufacturer's non-standard specific RNA-seq library protocol with poly-A capturing mRNA enrichment method (Illumina, CA, USA)
GEO Accession
GSM3326039