GSM3329543: T07_12h_miRNA_rep1; Euplotes vannus; miRNA-Seq
About 5 × 10^4 cells were collected on each treatment and homogenized in TRIzol Reagent, and RNA was extracted according to the manufacturer's protocol. Ethanol precipitation instead of RNA binding column was used in this isolation, which enables us to analyze both mRNA and microRNA from one sample. Small RNA libraries were generated using NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB, USA) from 1.5 μg total RNA.
GEO Accession
GSM3329543