GSM2843968: D3; Homo sapiens; miRNA-Seq
Total RNA was extracted using the miRNeasy Mini Kit (Qiagen, Venlo, The Netherlands) following the manufacturer's instructions. Micro Bio-SpinTM chromatography columns, supplied with Bio-Gel P-6 polyacrilyamide gel matrices, were applied to maximize purity of the RNA samples (Bio-Rad laboratories B.V. Veenendaal, The Netherlands). Small RNA libraries were generated from 2,000ng total RNA using TruSeq Small RNA Sample Preparation Kit and TruSeq small RNA indices (Illumina, San Diego, CA, USA).
GEO Accession
GSM2843968