miRNA-seq of Camellia sinensis: leaves infected by Lasiodiplodia theobromae
Samples from 15 plants at each treatment group were pooled together to avoid variations in individual plants due to pathogen inoculation. Leaf samples were ground into fine powder under the protection of liquid nitrogen. About 100 mg powder of each tea leaf was used for RNA extraction. The libraries of miRNA sequencing were prepared using TruSeq Small RNA Sample Prep Kits (Illumina, San Diego, USA) following the manufacturer's procedure. The libraries were sequenced on an Illumina Hiseq 2500 (LC Sceiences, USA) at the following the vendors recommended protocol.