GSM4630129: stage15a fetal skin scRNAseq; Felis catus; RNA-Seq
Embryos for single cell RNA sequencing were treated with dispase and the epidermis was removed with gentle scraping using a 15-blade scalpel. Epidermal sheets were dissociated with trypsin and gentle agitation. Red blood cells were removed with a red blood cell lysis solution. Single cell suspensions were incubated with a rat anti-CD49f antibody and enriched using a column-magnetic separation procedure. Libraries were constructed using 10x Genomics protocol for version2 (stage16a) or version3 (stage15a/b) chemistry
GEO Accession
GSM4630129