GSM4681743: RNAseq_Blister_replicate_1; Mus musculus; RNA-Seq
Suction-blistered and control samples were collected at P2 and treated with 0.25% trypsin EDTA overnight at 4°C. The epidermis was minced with a scalpel and suspended in 10% FCS DMEM. The cell suspension was filtered through a 70 µm filter, and cell pellets were collected. Library preparation was performed using an Illumina TruSeq RNA prep kit by following the manufacturer's instructions. Briefly, following TRIzol extraction and chemical fragmentation, mRNA was purified with oligo-dT-attached magnetic beads and reverse transcribed into cDNA. Following a second strand synthesis step with DNA polymerase I and RNAse H, the resulting cDNA was subjected to end repair, A-tailing, and Illumina compatible adaptor ligation. Following purification and PCR-mediated enrichment, libraries were purified with AMPure XP beads.
GEO Accession
GSM4681743