multiplex1
DNA extraction was performed with the JETFLEX Genomic DNA purification kit (GENOMED, L hne, Germany) following manufacturer s protocol. After quality assessment and quantification, DNA samples were submitted to Cornell University Genomic Diversity Facility at the Institute of Biotechnology (IGD, Ithaca, NY, US), where libraries were constructed and sequenced. GBS libraries were constructed and sequenced on an Illumina HiSeq 2000/2500 (100 bp, single-end) according to Elshire et al. (2011), using the restriction enzyme ApeKI (GC[A-T]GC) for digestion. A library with unique barcodes for each clone, plus blank samples, was created.BarcodeSampleAGGCSAL2AGCATTSAL19AATCGTTHYB9TCAAGTTPET4ACGTGTAPET24CAAGAAGTCAM2AACGTAGAATA16AGCTTGGTHMT13GATTSAL5CTCCGASAL20CTATGGAHYB10GATCATAPET9GATGCATHYR1GTCATGGTCAM4CTCACGGAATA17ACAGATGAHMT14ACCGTSAL7TTGGCAHYC1TACGGTAHYB11GCATTGAPET12CTAATGTHYR2AACAGTGACAM7TAGCGTGTATA18CAGTTGGTHMT15CGTCASAL8CCACGTHYC3ACTATGTHYB12CAGGTATPET14GTCGATAHYR3GTGCAAGACAM8ACGTAAGAATA19TGCAAGAAHMT16TCGCASAL9GATGTCHYC5CGTGAATHYB13TGCAATAPET15TATACGTHYR4CAATAGGACAM9CGTATGGTATA20ACTCGAGAHTU6CGCATSAL10TGTTACHYC6TTGCAGAHYB14ATATCGTPET16GCGTAATHYR5TGCAGTGTCAM13GTACGTGTATA21GGAGCTGTHTU7TCATAGTSAL12CAGTTAHYC7AACTTGTHYB15AGTCTATPET17AGCGTTAHYR7AGGCTAGAATA7TTCGAAGAATA26CTGAGTGTHTU8TTACGATSAL13GCCTATHYC8TGACGTAHYB17GTCTGAAPET18ATCCGGAHYR8CTAGTGGTATA11AATACGGABlankGATCAGAAHTU10GGCTAGASAL14AGTGGCHYC9GCTATAAHYB18ATCAGTTPET19TCAGTATHYR9GCTAGTGTATA12TGACTGGTHMT4TGCATAGAHTU11ACAATGGASAL16TGACCTHYC10ATCGTATHYB19CAGTTGAPET20CAATGTTHYR10AGTTGGCAATA13GCGGATGTHMT7GAACGAATHTU12ACAAGAGTSAL17TTGCACHYB6TACTGATHYB20TGTGCAAPET21GTTACGAHYR11TCGCAAGTATA14CATTGAGAHMT10TTGGCGGAHTU13GAACATGASAL18CTAGCTHYB7CTTGAGAPET3CGACAGTPET23TGCATATCAM1CGATGTGTATA15GTAACAGAHMT11CGCCGCATHTU15