GSM3701207: Meclizine replicate 2; Mus musculus; RNA-Seq
RNA was extracted from compound- or vehicle-treated cells in triplicate using Zymo Research Quick-RNA™ MiniPrep (Plus) kit (Zymo Research R1058) and RIN values were tested using Advanced Analytical. All samples had RIN values greater than 0.85. Libraries were prepared using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (New England Biolabs E7420L) and NEBNext® Poly(A) mRNA Magnetic Isolation Module kit (New England Biolabs E7490L). Libraries were multiplexed and sequenced on an Illumina Hi-Seq 2000 for single-end 50bp reads.
GEO Accession
GSM3701207