Illumina MiSeq sequencing; qiita_ptid_53:1716.McG.PAPrS20
From each plot, 25 soil cores (0-20cm) were randomly collected and composited as a representative sample for each plot. DNA from all soil samples was extracted with the EMP protocol. Soils have been thoroughly characterized in terms of physicochemical parameters. Additionally, fungal communities have been sequenced with Illumina (ITS region) and are positively and significantly correlated with increasing precipitation across the Isthmus.
EMP V4 515f,806rbc protocol
barcode
GAAAGGTGAGAA
center_name
CCME
center_project_name
mcguire_panamaprecipitationgradient_soil
illumina_technology
MiSeq
linker
GT
pcr_primers
FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
primer
GTGTGCCAGCMGCCGCGGTAA
run_center
CCME
run_date
2/14/12
run_prefix
McGuire_S0_L001
samp_size
.1,g
sample_center
McGuire Lab, New York NY
sequencing_meth
sequencing by synthesis
target_gene
16S rRNA
target_subfragment
V4