GSM1538840: 1001N; Homo sapiens; miRNA-Seq
Total RNA was extracted with TRIzol solution (Invitrogen),and the integrity of RNA was assessed using Agilent BioAnalyzer2100 (Agilent). Small RNA fraction (with length not exceeding 50 nt) was subjected to hybridization and ligation with Adaptor Mix (NewEngland Biolabs). Subsequently, RNAs were reverse transcribed and sequenced using whole-transcriptome sequencing on the IlluminaHiSeq2000 platform.
GEO Accession
GSM1538840