RNA-seq Huvec cells-TNF 10
Total RNA was isolated from 0.5x106HUVECs stimulated with TNFa for 0, 10 or 30 min using TRIzol (Invitrogen), treated with RQ1 DNase (1 unit/µg RNA; 37°C; 45 min; Promega), depleted of rRNA (RiboMinus; Epicentre), chemically fragmented to ~350 nt, and cDNA generated using random hexamers as primers (according to the True-seq protocol; Illumina). Adapters were then ligated to cDNA molecules, and libraries sequenced (Illumina HiSeq2000 platform; 100-bp paired-end reads; ~120x106 read-pairs per sample).