GSM2844197: 14 GW cortical plate after 16 hours of FFT culture subjected to control; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNAeasy Mini kit (Qiagen) according to the manufacturer's instructions. Polyadenylated mRNA was enriched with the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs) according to the manufacturer's instructions. The cDNA and second strand synthesis as well as the library preparation (consisting of cDNA fragmentation, end repair, A-tailing, ligation of sequencing adapters, indexing by PCR amplification for 15 cycles) were performed using the NEBNext Ultra II Directional RNA Library Prep Kit Illumina (New England Biolabs). Libraries were purified using XP beads (Beckman Coulter), quantified by Fragment Analyzer (AATI) and subjected to Illumina 75-bp single end sequencing on the Illumina Nextseq500 platform providing a minimum of 21 Mio reads per sample.
GEO Accession
GSM2844197