GSM3701388: katE_101373; Bacillus subtilis; RNA-Seq
Cells were mixed 1:1 with 50% Methanol and incubated on ice for 5 minutes before spin down for 10 minutes at 4°C. Methanol was removed, cells were lysed with lysozyme and RNA was purified using RNEasy kit as per manufacturers instruction (Qiagen) RNA was fragmented (ThermoFischer #AM8740), dephosphorylated, polyadenlyated, and reverse transcribed using Superscript III and custom oligo-dT reverse transcription primers. Resulting cDNA was ligated with Illumina sequencing adapters and PCR amplified. Single end (50 bp) sequencing was performed on an Illumina HiSeq 2000.
GEO Accession
GSM3701388