GSM1419770: S108; Mus musculus; RNA-Seq
Total RNA from area CA1 of the hippocampus was extracted, DNase-treated, and purified (RNeasy, Qiagen). 1µg of total RNA underwent quality control (Bioanalyzer; all RIN values > 7.9) and library construction for polyA+ RNA sequencing. Directional poly-A+ RNA llibraries were contructed at HudsonAlpha Genomic Services Lab using NEBNext reagents (New England Biolabs) according to manufacturer's recommendations with minor modifications (including the use of custom library adapters and indexes). RNA libraries were quantified (Kapa Library Quant Kit, Kapa Biosystems), and underwent sequencing (25M total 50bp paired-end reads) on an Illumina sequencing platform (HiSeq2000).
GEO Accession
GSM1419770