GSM1636034: Osr2RFP/+; Mus musculus; RNA-Seq
Palatal shelves were microdissected,digested with trypsin-EDTA solution at 37°C for 4 minutes. After inactivation of trypsin with DMEM containing 10% FBS, cells were dissociated by pipetting. The dissociated palatal cells were resuspended in PBS with 2% FBS and 10 mM EDTA, and filtered through a 40 μm nylon cell strainer, RFP+ cells were isolated using BD FACSAria II. RNA was extracted by Qiagen RNase Micro kit. Sequencing libraries were generated by using Illumina Nextera DNA Sample Prep kit. Sequencing libraries were generated by using Illumina Nextera DNA Sample Prep kit.
GEO Accession
GSM1636034