GSM3759478: Zn plus shoot-rep2 miRNA-seq; Oryza sativa Japonica Group; miRNA-Seq
Rice shoots and roots were collected after Zn-deficiency and Zn-resupply treatments. Eighteen samples (Zn-plus shoot, Zn-minus shoot, Zn-resupply shoot, Zn-plus root, Zn-minus root, Zn-resupply root, each with three biological replicates) were harvested for small RNA library construction and sequencing. Roots and shoots from three rice seedlings were collected and mixed together as one sample. Total RNAs were extracted from samples using Trizol reagent (Invitrogen, Carlsbad, CA). RNA abundances and purity were tested following the manufacture of RNA 6000 Nano LabChip Kit (Agilent, CA, USA). Approximately 1.0 μg of total RNA was used to prepare a small RNA library according to the protocol of TruSeq Small RNA Sample Prep Kits (Illumina, San Diego, USA). Single-end sequencing with a length of about 50 bp was conducted on an Illumina Hiseq2500 at the LC-BIO (Hangzhou, China).
GEO Accession
GSM3759478