GSM3177657: in 1x3hr in vivo +TNFα rep2; Mus musculus; RNA-Seq
RNA was extracted using RNeasy Plus Micro Kit (Qiagen). RNA integrity number (RIN) was determined by Bioanalyzer (Agilent Technologies) and RNA samples with RIN > 8.0 were subjected to further processing. The double-stranded cDNA was generated using Ovation RNA-Seq System V2 (Nugen), and the sequencing libraries were prepared using LTP Library Preparation Kit (Kapa Biosystems). Different adaptors were used for multiplexing samples in one lane.
GEO Accession
GSM3177657