sRNA seq of Beauveria bassiana mycelia collected from SDAY plates after 4 days of growth
Beauveria bassiana mycelia growthed on the SDAY plates for 4 days were collected to extract total RNA. Total RNA was used to construct sRNA library as the following step. (1) Filter sRNA: separate 18~30nt RNA segment by PAGE gel. (2) 3' adaptor ligation. (3) Reverse primer annealing. (4) 5' adaptor ligation. (5) Strand cDNA synthesis. (6) PCR amplification. (7) Library fragment selection. (8) Library quantitative and pooling cyclization.