GSM2585781: Nac_wt_1_Total; Mus musculus; RNA-Seq
Nac and DStr were removed and homogenized in polysome lysis buffer. Fifty microliters of the initial lysate were used for RNA extraction using Trizol protocol according to manufacturer's instructions (here referred as 'Total') and the rest of the lysate was then centrifuged in 10-50% sucrose density gradients. Ribosome profiling was then performed, samples were fractionated and RNA was isolated from heavy polysomal fractions (>4 ribosomes) using Trizol protocol (here referred as 'Heavy'). RNA-Seq libraries were prepared with the 'TruSeq Stranded RNA sample preparation Kit' (Illumina) according to the manufacturer's instructions.
GEO Accession
GSM2585781