GSM4194886: 2279; Mus musculus; RNA-Seq
RNA was isolated from 25 mg of liver using the RNEasy Mini Kit  according to the manufacturer's protocol (Qiagen, Valencia, CA). RNA purity and quality were assessed using the 2100 Bioanalyzer (Agilent, Santa Clara, CA) and RNA concentration was quantified using a Cytation 3 spectophotomerter (Biotek, Winooski, VT). Samples with an RNA Integrity Number (RIN) < 8 or 260/280 < 1.8 were excluded from further processing. Extracted total RNA was used to prepare RNA-seq libraries using the TruSeq RNA Library Preparation kit v2 according to the manufacturer's protocol and then sequenced using the Illumina HiSeq 2000
GEO Accession
GSM4194886