RNA-seq of Aedes aegypti: abdomen of an A. aegypti female mosquito
The RNA was extracted from abdomen of an Aedes aegypti female mosquito, which was initially macerated and subsequently subjected to extraction using the QIAamp viral RNA mini kit (Qiagen, Valencia, CA, USA), purified (with DNase I) and concentrated using the RNA Clean & Concentrator TM-5 kit (Zymo Research, Irvine, CA, USA). The RNA was submitted to next-generation genomic sequencing. Briefly, the paired-end RNA libraries were constructed using the TruSeq Stranded Total RNA HT sample prep kit, and the sequencing was done using the Illumina NextSeq platform.