GSM3328094: Pig 5; Sus scrofa; miRNA-Seq
EV were isolated from milk samples as described in Zonneveld et al, J. Extracell Vesicles 2014, and van Herwijnen et al., Mol. Cell. Proteomics, 2016. RNA was isolated from the EV using the miRNeasy micro kit (Qiagen) NebNext with following adaptations (for human): overnight 3' adapter ligation, amplification with 2x Kapa HiFi PCR mastermix (15 cycles). Illumina Truseq (for pig) with 17 PCR amplification cycles, followed by TBE gel cleanup
GEO Accession
GSM3328094