GSM2424639: crp.ee.chipseq; Escherichia coli str. K-12 substr. MG1655; ChIP-Seq
Cells were fixed in the required growth phase in formaldehyde, quenched, washed and lysed. Fragmented DNA - protein complex were pulled using the monoclonal flag tag antibody. The complex was then washed, reverse cross-linked and obtained DNA purified using Qiagen min elute PCR purification kit. Truseq ChIP kit was used for the library preparation. DNA was end repaired and adapters were ligated. After ligation, purify ligation products and then amplify.
GEO Accession
GSM2424639