GSM3244931: K-Ras Mll4-/- _RNAseq_rep2; Mus musculus; RNA-Seq
Total RNA was prepared from CD45-depleted K-Ras and K-Ras Mll4-/- lung tumors using Trizol reagent (Life Technologies).Briefly, to isolate total RNA from lung tumor tissues, first tumor cells were enriched by depletion of CD-45 positive cells. The tumor tissues were dissected and cut into <1mm pieces. The tissues were then digested in Collagenase type 1 and DNAase1 for 45 min followed by 0.25% trypsin for 10 min. To remove RBC, the digested single cells suspension then treated with RBC lysis buffer for 2-3 min. CD45-positive cells were then depleted using MagniSort™ Mouse CD45 Depletion Kit (ThermoFisher Scientific). Total RNA was isolated using Trizol reagent (Life Technologies). RNA samples were sequenced using the Illumina HiSeq 2000. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM3244931