Illumina MiSeq paired end sequencing
To use shotgun metagenomics to capture BK polyomavirus, as well as all other taxa within the urine sample.
Libraries were prepared using the Nextera XT library preparation kit (Illumina). Libraries were sized by Bioanalyser (Agilent) and quantified using the KAPA Illumina library quantification kit (KAPA Biosystems), and then sequenced by paired end sequencing on the Illumina MiSeq instrument using the V3 150-cycle reagent kit (Illumina).