GSM2585902: WT_LivTotRNA_ZT12; Mus musculus; RNA-Seq
One portion of the liver was snap frozen to isolate total RNA, the rest of the tissue was homogenized to isolate liver nuclei and extract nuclear RNA. mRNA from either total liver tissue or liver nuclei was purified using Trizol reagent (Invitrogen) Libraries were prepared using Illumina’s TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold according to manufacturer’s instructions
GEO Accession
GSM2585902