GSM2844173: Tuner_2A; Homo sapiens; RNA-Seq
RNA was extracted using the Trizol reagent and a QC was conducted using the Agilent Bioanalyzer. Total RNA was treated with Dnase I and mRNA was enriched using the oligo (dT) magnetic beads. mRNA was fragmented into short fragments and cDNA was synthesized using random hexamer primer. The double strand cDNA was purified with magentic beads. End reparation and 3'-end single nucleotide adenine addition was performed. Finally, sequencing adaptors were ligated to the fragments. The fragments were enriched by PCR amplification. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM2844173