GSM3176653: +TIR +auxin +DTT 2hr fp; Saccharomyces cerevisiae; OTHER
For ribosome profiling, cells were flash frozen and mixermilled at -80 together with ribosome profiling lysis buffer, extract was clarified with a low and high speed spin, all as described in Ingolia et. al, Science, 2009. For mRNA, cells were flash frozen and lysed using hot acid phenol and SDS as described in Ingolia et al., Science, 2009 As described in Ingolia et al, Science 2009
GEO Accession
GSM3176653