sort C mouse immunoglobulin-bound gut microbiome V4 16S sequence reads
Mouse serum, cecal sample, and fluorescent anti-immunoglobulin antibodies combined and flow-sorted. All combinations of immunoglobulin highs/lows sorted into tubes. Tubes sent to Baylor College of Medicine for DNA extraction V4 16S amplicon amplification and PE 250bp Illumina MiSeq sequencing. Reads have been demultiplexed, quality-filtered, and merged (using SeqPrep). Target amplicon size was ~250bp, so each sequence has ~2x coverage.