GSM4632935: D14_ATACseq; Homo sapiens; ATAC-seq
Briefly, 50,000 cells were detached from each induction plate at the previously defined time-points and washed with cold PBS. Next 50 μl of cold lysis buffer was added to the cell pellet. The Tn5-mediated tagmentation and adapter incorporation were performed with the Nextera DNA Library Prep Kit (Illumina) following manufacturer's protocol at 37ºC for 30 min. The transposed DNA was purified using Qiagen MinElute PCR Purification Kit (Qiagen). To amplify DNA fragments, we used NEBNext High-Fidelity PCR Master Mix (New England Biolabs).
GEO Accession
GSM4632935