GSM2770694: wild type (parental) HAP1 cells biological replicate 3; Homo sapiens; RNA-Seq
RNA was isolated from HAP1 cells using the TRIzol reagent (Invitrogen) according to manufacturer’s instructions. RNA yields were determined using a NanoDrop spectrophotometer (Wilmington, Delaware, USA) and RNA quality was assessed by Agilent Bioanalyzer RNA 600 Nano Kit. Only samples with RIN values above 9 were accepted. Library generation including random fragmentation, cDNA synthesis and sequencing was performed at the VIB Nucleomics Core (www.nucleomics.be) using the TruSeq stranded total RNA sample preparation kit (Illumina, San Diego, California, USA) and including a Ribo-Zero (Illumina) depletion step as to remove ribosomal RNA from total RNA by the use of biotinylated Ribo-Zero oligos. Libraries were subjected to sequencing on a NextSeq 500 instrument (Illumina) to yield 75 bp single-end reads, resulting in approximately 23-28 million 75 bp single end reads per sample.
GEO Accession
GSM2770694