GSM2424386: cond_2_rep2; Schizosaccharomyces pombe; RNA-Seq
RNA was extracted from the cells by bead beating with Trizol (Ambion), followed by isopropyl alcohol precipitation and ethanol wash. To remove ribosomal RNA, we input 1ug of total RNA into the Ribo-Zero™ Magnetic Gold Kit (Yeast) Illumina cat #MRZY1324) followed by a cleanup using Agencourt RNAClean XP beads (Beckman Coulter, cat #A63987) and elution with 19.5 uL of Elute, Prime, Fragment mix from the TruSeq RNA Sample Preparation Kit (Illumina, cat #RS-122-2101). We performed library construction per the vendor’s instructions, starting with the “Incubate RFP” step. We pooled the resulting cDNA libraries and subjected them to paired-end sequencing on an Illumina miSeq --151  base pairs per read.
GEO Accession
GSM2424386