GSM4633142: HT29_LM_3; Homo sapiens; ATAC-seq
50,000 (viability >90%) cells were pelleted at 500 RCF at 4°C for 5 min. Cell pellet was resuspended in cold ATAC-Resuspension Buffer (RSB) (0.1% NP40, 0.1% Tween-20, and 0.01% Digitonin) and incubated on ice. RSB was washed out with cold ATAC-RSB (containing 0.1% Tween-20 but NO NP40 or digitonin). Nuclei were pelleted at 500 RCF for 10 min at 4°C. Cell pellet was resuspended in 50 ul of transposition mixture (25 ul 2x TD buffer, 2.5 ul transposase (100nM final), 0.5 ul 1% digitonin, 0.5 ul 10% Tween-20, 5 ul H2O). Reaction was incubated 37°C for 30 minutes in a thermomixer with 1000 RPM mixing. The tagmented nuclei was sequenced using an Illumina.
GEO Accession
GSM4633142