MiSeq of sulphate reducing bacterial: acid mine drainage wastewater
Data was trimmed and only >q20 (i.e high quality) reads were used. Every read was BLASTED and the result file savedThe top hit for every BLAST result (i.e genus and species name) was counted and a record was kept of how many times each species appeared as a hit. If in the event that no BLAST result was found for a particular read, we recorded that read count under 'No Hits'Taxa information for every BLAST hit was recorded