GSM2230758: human pancreatic islets, sample 2; Homo sapiens; RNA-Seq
Cells were encapsulated using the inDrop platform, into droplets on ice and lysed  in the 4nL microfluidic droplets using a final concentration of 0.4% NP-40. Single cell lysates were subject to reverse transcription at 50°C without purification of RNA (as previously described in Klein et al., Cell 2015). Cells were barcoded using the inDrop platform (Klein et al., Cell 2015), which makes use of the CEL-Seq protocol for library construction Hashimshony et al., Cell Reports 2012)
GEO Accession
GSM2230758