GSM3177373: DIPG29-normal_cortex; Homo sapiens; RNA-Seq
RNA-seq samples were pelleted and lysed in Trizol reagent then subsequently precipitated. After extraction from Trizol, RNA-seq samples were linearly amplified using the Ovation RNAseq v2 kit (NuGEN) and fragmented with a Covaris sonicator. Libraries were end-repaired using T4 DNA Polymerase, E. coli Klenow fragment, and T4 polynucleotide kinase. DNA was then A-tailed using exo(-) Klenow fragment and ligated to adaptors from NEBNext MultiPlex Oligos using New England BioLabs Quick Ligase. Libraries were sequenced at 1x75 on an Illumina NextSeq 500
GEO Accession
GSM3177373