GSM3176055: WTCHG_319032_708503; Homo sapiens; RNA-Seq
RNA was extracted with trizol (Invitrogen) using a linear acrylamide carrier. RNA was then treated with DNase I (Life technologies) and purified using Agencourt RNAClean XP beads (Beckman Coulter). RNA quality and concentration were assessed by using an RNA 6000 bioanalyzer pico kit (Agilent). Samples with concentrations less than 30 pg/µl and/or an RNA integrity number (RIN) less than 6 were excluded from further analysis. A cDNA library was produced using the Smart-Seq 2 protocol. Sequencing was performed on an Illumina HiSeq4000 with 75 bp paired-end reads with the aim to achieve coverage of 25 million reads per sample.
GEO Accession
GSM3176055