GSM1310903: Not treated with 4-NQO [SAMPLE 9]; Mus musculus; RNA-Seq
RNA was extracted with QIAGEN RNeasy mini kit and cleaned up wiith DNase I treatment in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser. RNA sequencing was performed using Illumina TruSeqRNA technology, following the manufacturer’s protocol. Briefly, mRNA is purified using poly-T oligo-attached magnetic beads and subsequently fragmented. The cleaved RNA fragments are reverse transcribed into first strand cDNA using random primers. Next, second strand cDNA is synthesized using DNA Polymerase I and RNase H. The second strand cDNA fragments are subject to an end repair, the addition of a single ‘A’ base, and ligation of the adapters. The products are then purified and enriched with PCR to create the final cDNA library.
GEO Accession
GSM1310903