GSM2155071: ChIP-seq of Pol II in YA animals at 34°C, replicate 2; Caenorhabditis elegans; ChIP-Seq
Worms were collected and washed in cold M9 buffer, crosslinked with 2% formaldehyde at room temperature for 15 min., re-suspended in FA buffer, lysed by douncing in a Kontes 2 ml glass dounce and sonicated in Bioruptor to yield 200 bp–800 bp size DNA fragments. Extracts corresponding to 200 μg of protein from L2 Larvae or 400 μg of protein from young adults was used for each pull-down. For ChIP-seq analysis of HSF-1 and Pol II, 5 μl of antibody against GFP (clontech, polyclonal) or 2.5 μl of antibody against Pol II (Novus) was used in each immunoprecipitation. DNA was pooled from 6 immunoprecipitations for each ChIP-seq experiment. Libraries were prepared following PrepX DNA library protocol with Appollo 324 system.
GEO Accession
GSM2155071