HFD4 mice intestinal metagenome
This study was conducted to characterize the mice intestinal microbiota at different feeding conditions includes: the mice were fed standard diet (CTL), diet containing 45%Kcal fat diet (HF) and HF containing 1% laminarin (HFL) for four weeks. Total DNA was extracted from 150 mg of fecal materials using MOBIO Power Fecal DNA isolation kit (MO BIO Laboratories Inc., CA USA). The obtained DNAs were pooled by each treatment groups at week 4 (CTL4, HF4, and HFL4) and week 6 (HFL6) for metagenome shortgun sequencing. Library construction and sequencing were performed with Illumina HiSeq 2000 (100bp x 2) according to the manufacture’s instruction.
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PHRED_33