Spontaneous rough colony mitotic derivative of JAY270 (paired)
JAY270 and independent rough colony isolates were grown overnight at 30C in eight ml YPD broth. Full length chromosomal DNA embedded in agarose was prepared for pulse field electrophoresis (PFGE). DNA from the PFGE agarose blocks was sheared by sonication  and purified using a Fermentas gel extraction column kit. Sheared DNA was size-selected on agarose electrophoresis gels. DNA fragments of approximately 400 bp in size were excised from the gel  purified with a Fermentas gel extraction column kit  and used as template for sequencing library preparation. Whole genome sequencing on Illumina platform was performed at University of North Carolina High Throughput Sequencing Facility.