GSM2232115: G_122_48; Mus musculus; RNA-Seq
Groups of mice infected with strain 201 or EV76 were killed at 12, 48 and 72 hpi, and lungs, liver and spleen of infected mice were collected. Approximately 100 mg of tissue was immediately immersed into 1.5 ml RNAlater solution. Samples were then stored at 4 ºC for RNA isolation experiments. Pools of lungs, liver and spleen tissues containing at least 8 mice per duration of infection were homogenized in homogenizer. TTotal RNA were purified from the lungs, liver and spleen using a TRIzol reagent  and treated with RNAse free DNase I. Agilent 2100 Bioanalyzer and the Agilent RNA 6000 Nano kit were used to detect RNA integrity number, rRNA ratio (28S/18S), and the concentration of the total RNA samples. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM2232115