RNAseq of liver to identify MUPs in house mice and wild relatives
NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #E7530). NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) was used for RNA Isolation. Sequences were indexed using the NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1, NEB #E7600). We sequenced paired end libraries at the Institute for Biotechnology at Cornell University. Some libraries were sequenced on a MiSeq (PE 300) while others were sequenced on a NextSeq (PE 150).