GSM2586533: Mll3/4_ChIP-seq_WT_rep2; Mus musculus; ChIP-Seq
Nuclei were extracted and chromatin sonicated to 250-500bp with a Covaris sonicator and IP was performed with antibody as indicated overnight. ChIP DNA, input DNA and cDNA were subject to end repair, A-tailing, adaptor ligation and cleavage with USER enzyme, followed by size selection of 250-500 bp and amplification with NEBNext sequencing primers
GEO Accession
GSM2586533