GSM1904301: LPS/Nec-1 (Treated); Mus musculus; RNA-Seq
bone marrows were collected from the femurs by flushing them with PBS followed by centrifugation at 430 g then resuspended and blocked with 1 μM EDTA and 2% BSA in PBS for 45 min at 40C, then stained using anti-CD11b+-PE antibody (Biolegend, clone M1/70, 1:500) for 60 min at 40C and isolated by FACS sorting using MoFlo sorter (Beckman-Coulter). Input RNA was first amplified and converted to cDNA using NuGen Ovation V2 per the manufcaturer instruction. After cDNA synthesis, S1 nuclease was used per instruction protocol (Promega).  The treated cDNA was then sheared and use as input for library preparation using Illumina TruSeq DNA Sample Preparation Kit (Sample:Index C2-1:GGCTAC, C3-1:GTGGCC, T2-1:GAGTGG, T3-1:ACTGAT, V2-1:GTTTCG V3-1:CGTACG)
GEO Accession
GSM1904301