GSM3758236: UACC-DPA-B; Homo sapiens; OTHER
Genomic DNA from cell pellets was purified using the QIAamp DNA Blood Maxi/Midi/Mini kits (Qiagen) according to the manufacturer's protocols and quantified using a Nanodrop 2000 (Thermo Fisher Scientific). sgRNA cassettes were PCR-amplified and barcoded with sequencing adaptors utilizing ExTaq DNA Polymerase (Clontech). PCR products were purified with Agencourt AMPure XP SPRI beads (Beckman Coulter A63880) according to the manufacturer's instructions, quantified using a Nanodrop 2000, pooled into a master sequencing pool, and sequenced (Illumina) with 300nt single-end reads, loaded at 60% with a 5% spike-in of PhiX DNA.
GEO Accession
GSM3758236