GSM3258308: TRIM-30-mpw-B; Xenopus laevis; RNA-Seq
RNA was isolated using TRIreagent (Sigma) following the manufacturer's protocol. Diluted RNA was treated with DNAse I solution (Sigma), followed by an overnight reprecipitation during incubation at -20 °C using a 4 M final concentration of  LiCl solution . RNA-Seq was performed using biological duplicates. Kit from NewEnglandBiolabs (NEB #E7490S) was used for poly-A selection of RNA using 500 ng of total RNA. Libraries were prepared by NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB #E7420S) according to manufacturer's protocol and sequenced on the NextSeq 550 in 2x75bp HighOutput mode.
GEO Accession
GSM3258308