GSM4568660: RNA_seq_SingleCell_NP_dermis; Mus musculus; RNA-Seq
Subcutaneous fat was removed from the skin with a scalpel, and the whole skin was incubated for 30 min in 0.25% trypsin to separate into epidermis and dermis. Dermal cells were dissociated into single cells by pipetting and filtrated on a 40μm (Falcon) and 20μm (PLS). Single cell suspensions were resuspended in 1% BSA/PBS at 1000 cells/µL and barcoded with a 10× Chromium Controller (10× Genomics). RNA from the barcoded cells of each sample was subsequently reverse transcribed, and sequencing libraries were constructed with reagents from a Chromium Single Cell v2 reagent kit (10× Genomics), according to the manufacturer's instructions. Sequencing was performed with a NextSeq 500 (Illumina), according to the manufacturer's instructions.
GEO Accession
GSM4568660