GSM3759417: RNAseq 12M NP WT; Mus musculus; RNA-Seq
We purified the pancreatic islets (Kuo et al, JBC 2016) and FAC-sorted the beta cells. RNA was isolated with Nucleospin RNA kit (Macherey-Nagel) with DNase I treatment. Directional poly-A RNAseq libraries were prepared and sequenced as PE100 (100bp paired-end reads) on Illumina Hiseq 2500 in high output mode.
GEO Accession
GSM3759417