Bacterial V3V4 16S rRNA amplicon from silk sample obtained from crypt no 3 in the Chapel of the Passion of Christ (fabric from the bottom of the crypt)
3x total DNA isolation x 3x PCR with the use of 16S_V3-F and 16S_V4-R primers of bacterial V3V4 16S rRNA region mixed and sequenced with 2x300 chemistry