GSM1904376: Site 12, 11/25/2011, water; riverine metagenome; OTHER
Total DNA was extracted from filtered (HT-200 polysulfone 0.2 µm filter membranes) samples using a FastDNA® Soil Spin Kit (MP Biomedicals). The V4 region of the 16S rRNA gene was amplified using 501-508 and 701-712 primers as previously described (Kozich et al., 2013, Appl Environ Microbiol 81:5112-5120). The library was quantitated by qPCR and sequenced on one MiSeq Nano flowcell for 251 cycles from each end of the fragments using a MiSeq sequencing kit version 2 and analyzed with Casava1.8 (pipeline 1.8). PhiX DNA is used as a spikein control for MiSeq runs.  Most PhiX reads are removed in normal Casava processing but a few residual reads may remain. PhiX genome is 3kb in length.
GEO Accession
GSM1904376