GSM4568910: scRNA-seq_gata2b het 1; Danio rerio; RNA-Seq
After euthanasia, the adult zebrafish were subjected to cardiac bleeding to remove most mature erythrocytes from the marrow. The zebrafish were then dissected at room temperature and the dissected marrow was placed into 500ul of 0.9x PBS with 2% FBS and 1 USP units/mL heparin, placed on ice. The marrow samples were then mechanically dissociated by repeated pipetting into single cell suspension and passed through a 40-μm nylon mesh. The samples were sorted into 1x PBS with 0.04% BSA while kept cold at 4C (both sample tube being sorted and collection tube). Samples were dissected and sorted in a staggered fashion, every 45-60min, were kept on ice after sorting and walked to the processing core every 45-60min for processing for inDrops single cell RNA sequencing. Library preparation with inDrops v3 scRNA sequencing was performed by the Harvard Medical School single-cell core.  https://github.com/indrops/indrops
GEO Accession
GSM4568910