GSM2157061: Westar_24hpi_1; Brassica napus; RNA-Seq
RNA was isolated using Invitrogen Plant RNA Purification Reagent, and subsequently treated with the Ambion Turbo DNA-free DNase Kit according to the manufacturer’s protocol. Quantity and purity were assessed spectrophotometrically and quality of RNA samples was verified with electropherogram profiles and RNA Integrity Numbers (RIN) using an Agilent 2100 Bioanalyzer and RNA Nano Chip RNA-sequencing libraries were prepared according to alternative HTR protocol (C2) established by R. Kumar et al. (2012) with the exception of library PCR enrichment that was carried out using 11 PCR cycles. RNA sequencing libraries were validated using the Agilent Bioanalyzer DNA chips (Agilent Technologies) and quantified using the Quant-iT dsDNA Assay kit (ThermoFisher Scientific).
GEO Accession
GSM2157061