GSM3259827: b2-DHFR - 30 min - Replicate 2; Saccharomyces cerevisiae; RNA-Seq
Total RNA was purified from clarified cell lysates using phenol-chloroform extraction. DNA was removed with the Turbo DNA free kit (Ambion). Barcoded stranded mRNA-seq libraries were prepared from total RNA (300 ng) using the Illumina TruSeq RNA Sample Preparation v2 Kit (Illumina, San Diego, CA, USA) implemented on the liquid handling robot Beckman FXP2.