GSM4084081: HYNUNtx_mms7712+liver; Hynobius unisacculus; RNA-Seq
The extraction of total RNA was done by lysing the embryos in Trizol reagent (Invitrogen), and purified with Direct-Zol RNA purification kit (Zymo) RNA sequencing library was constructed according to manufacturer's instructions (Illumina, San Diego, CA, USA) after polyA enrichment.