GSM4010354: s3: pdgfb_ret_wt_cortex_rep2; Mus musculus; RNA-Seq
Brain calcifications are autofluorescent (Keller et al., 2013) and we exploited this characteristic to identify calcifications under a fluorescent stereomicroscope. Calcifications were surgically isolated from the brains of Pdgfb ret/ret animals. Brain tissue from the same anatomical regions was isolated from control animals. In addition, we also isolated tissue from non-calcification prone brain region (cortex) from both Pdgfb ret/ret and control animals. RNA was isolated using RNeasy micro kit (Qiagen) according to the manufacturer's instructions. RNA samples were polyA-enriched and libraries were prepared using the Illumina TruSeq® Stranded RNA kit.