GSM4083317: lrgE122: Large RNA EVs ATG12-/- Biorep #2; Homo sapiens; RNA-Seq
Total RNA from wild-type, ATG7-/- and ATG12-/- HEK293T EVs and cells was isolated using the Macherey-Nagel NucleoSpin kit (# 740971) according to the manufacturer's protocol. Large RNA libraries were prepared using the SMARTer Stranded Total RNA Sample Prep Kit - HI (Takara # 634875), purified using SPRI AMPure Beads (Beckman Coulter, #A63880), and amplified for 22 cycles. RNA-seq: Single-end 50 bp read length on a HiSeq4000