GSM3763297: SUM159_H1_P5_DMSO; Homo sapiens; OTHER
For the pooled genome-wide CRISPR screen, 100 million SUM149 and SUM159 Parental and resistant cells were infected with the pooled lentiviral H1 and H2 libraries essentially as described (Jeselsohn et al., 2018) at a multiplicity of infection of 0.3. After 3 d of puromycin selection, half of the surviving cells were stored as 0-d control samples, and the rest of cells were cultured for an additional 5 passages with or without different concentrations of JQ1 treatment. PCR was performed on genomic DNA to construct the sequencing library.