RNA-seq of pea: dorsal petal
For Illumina sequencing, after poly (A) mRNA was purified and fragmented into small pieces, we used random hexamer primers and reverse transcriptase (Invitrogen) to carry out first-strand cDNA synthesis. Second-strand cDNA synthesis was performed with RNase H (Invitrogen) and DNA polymerase I (New England BioLabs).
TereseDp