Metagenomics of Human skin sample above panniculus: Sample Boom10o
"Cotton swabs moistened with 0.15 M NaCl + 0.1% Tween 20 were used to collect samples, one from the anterior surface of the panniculus (3-4 cm inferior to the umbilicus) and from the intertriginous area under the panniculus. These swab samples were immediately stored at -80C. Samples were thawed, and microbial genomic DNA was isolated using the Fecal DNA isolation kit from Zymo Research (catalog #D6010).Once the sample DNA was prepared, PCR was used with unique bar coded primers to prepare the 16S rDNA V4 region amplicon library. PCR products were purified by gel electrophoresis, quantified, and sequenced using an Illumina MiSeq. Illumina sequencing generated paired-end reads of 251 bases."
Boom10o