Targeted sequencing of Plasma from patient BMBC1 using the Breast Cancer targeted sequencing panel
Targeted sequencing was performed using a customized breast cancer panel, targeting all exons of 254 genes recurrently mutated in breast cancer and related to DNA repair. 40-450ng of DNA was used to prepare barcoded sequence libraries (New England Biolabs, Kapa Biosystems), which were pooled at equimolar concentrations for hybridization exon capture (Nimblegen SeqCap).
BMBC1_plasma_BreastCancerPanel