GSM1544020: ABA5_miRNA; Fragaria x ananassa; RNA-Seq
Transcriptome: Total RNA was isolated using Trizol Reagent (Invitrogen Life Technologies, USA) according to the manufacturer’s instructions. RNA samples were treated with RNase free DNase I (Takara, Japan) to avoid genomic DNA contamination. For each group, samples from 10 individual fruits were randomly selected and RNA was extracted separately. Before equal amount pooling, the total RNA quantity and concentration were tested on Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RNA integrity number (RIN) higher than 8.0. Transcriptome: Paired-end Illumina mRNA libraries were generated in accordance with the manufacturer’s instructions for mRNA-Seq Sample Preparation (Illumina Inc., San Diego, CA, USA). The 7 libraries were subjected to sequencing on an Illumina Hiseq 2000 platform and 100 bp paired-end reads were generated. miRNA: Seven small RNA libraries of samples as mentioned above were prepared for sRNA analysis according to protocol of TruSeq Small RNA Sample Prep Kits (Illumina, San Diego, USA). miRNA: Single end sequencing (36 bp) was performed on an Illumina Hiseq 2500 at the LC-BIO (Hangzhou, China) following the vendor’s recommended protocol. degradome:7 degradome cDNA libraries were constructed following the procedures previously described by German et al. (2008).