GSM4633903: sljmj7_rep3; Solanum lycopersicum; Bisulfite-Seq
5 g tomato sample were used to extract DNA. The qualified DNA was fragmented with an ultrasonic disruptor (Bioruptor), with an average size of about 300-500 bp. Fragmented DNA were end-repaired and ligated to a fully methylated adapter by using NEXTflex™ Bisulfite-Seq Barcodes-6 Kit (51191, Bioo Scientific). Using EZ DNA Methylation-Gold ™ kit (Zymo Research Corp., catalog number D5005) for bisulfite conversion of adapter-linked DNA. After amplification by PCR, the high-quality library has a DNA fragment distribution of about 300 bp