GSM2344465: RNA UT_2; Mus musculus; RNA-Seq
Nascent 4-thio-uridine (4sU) labelled RNA was obtained adding 4sU to the cell culture medium at a final concentration of 300 mM for 45 min before harvesting the cells. The 4sU-labeled RNA was isoleted ad described (Rabani M. et al., 2011).Chromatin associated RNA was prepared as previously described (Bhatt et al., Cell 2012). Libraries were prepared from 40 ng of  RNA using the TruSeq RNA sample preparation kit (Illumina), skipping the step of either ribosomal depletion and polyA-selection.