GSM4237285: spinal motor neurons, WT-4; Mus musculus; RNA-Seq
The spinal cord  were rapidly dissected from the embryo day 12.5. The isolated tissue was dissociated using Neural Tissue Dissociation Kit (Miltenyi Biotec). Motor neurons were sorted using FACS AriaTM Fusion Flow Cytometer (Becton Dickinson). The cells were directly collected in RLT lysis Buffer (Qiagen). Total RNA extracted using the RNeasy Mini Kit (Qiagen) including DNase treatment. cDNA libraries were prepared using NeuGen Ovation® SoLo kit.