GSM1135018: C-Input-1; Homo sapiens; OTHER
At 48-h post-transfection, total RNA was isolated from transfected cells with TRIZOL reagent. Poly-adenylated RNA was enriched by using FastTrack MAG Maxi mRNA isolation kit (Invitrogen). RNA fragmentation, m6A-seq, and library preparation were performed as in Dominissini et al 2013 Nature Protocols. TruSeq RNA Sample Preparation Kit (Illumina) refer to specific columns refer to specific columns