GSM4008522: 04_3990Michigan_DIPG-007_HK2shRNA; Homo sapiens; ATAC-seq
For ATAC-Seq 100,000 cells were collected by centrifugation at 500g for 5min. Cell pellets were resuspended in lysis buffer, pelleted, and tagmented using the enzyme and buffer provided in the Nextera Library Prep Kit (Illumina). Tagmented DNA was then purified using the MinElute PCR purification kit (Qiagen), amplified with 10 cycles of PCR, and purified using Agencourt AMPure SPRI beads (Beckman Coulter).