16S rRNA Sequences (V4 region) from Cathode-Oxidizing Communities Enriched from Marine Sediment Raw sequence reads
Illumina MiSeq paired-end (2 x 250 bp) sequencing was performed on the V4 hypervariable region of the 16S rRNA gene of extracted DNA. In summary, ribosomal sequences were amplified with a 30-cycle PCR reaction using the HotStarTaq Plus Master Mix Kit (Qiagen, Carlsbad, CA) with a 53ÁC annealing temp using barcoded 515F (5Í-GTG CCA GCM GCC GCG GTA A) primer paired with 806R (5Í-GGA CTA CHV GGG TWT CTA AT) primer. Amplicons were pooled and purified with calibrated Agencourt Ampure XP beads (Agencourt Bioscience Corporation, Beverly, MA). Purified PCR product was used to prepare DNA libraries by following the Illumina TruSeq DNA library preparation protocol and sequenced on an Illumina MiSeq following manufacturerÍs guidelines (Illumina Inc., San Diego, CA).
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