The Metabolic Regulation of Adult Reproductive Diapause in Drosophila suzukii (Diptera: Drosophilidae) Females by Transcriptomic
A field population of D. suzukii was collected from cherry orchards in Shandong Province. The strain was reared in a continuous laboratory culture on artificial medium. The insects were maintained in the laboratory at 25¡À1 ¡ãC under a 16L: 8D daylight cycle. Female flies were collected within 12 h after eclosion and reared on an artificial at 10¡À1 ¡ãC for 15 days, and a photoperiod of long-day length (16L: 8D) to generate non-diapause females and short-day length (8L: 16D) to generate diapause females. The principle criterion for reproductive diapause in the fruit fly is the developmental status of the ovaries. Two biological replicate samples were stored at -80 ¡ãC until use, and each sample was used for whole-genome transcriptomics analyses.
SWD-Diapause-1