RNAseq of Homo sapiens: Colorectal cancer myeloid subsets
One thousand I-MDSCs, M-MDSCs, PMN-MDSCs and monocytic APCs cells were sorted with high purity from PBMC of CRC patients. The cDNA libraries were prepared from sorted cells using QIAseq FX Single Cell RNA Library Kit (Qiagen, Hilden, Germany). The quality passed libraries were subjected to sequencing using illumina HiSeq 4000 as previously described.
CRC 09 GMC