Exploring bacteriophage genome to understand pathogen resistance
Library preparation was performed at Genotypic Technology’s Genomics facility following Sure SelectQXT Library Prep protocol outlined in Sure SelectQXT Whole genome Library Prep for Illumina Multiplexed sequencing Protocol (Cat # 5500-0120). 25ng of Qubit quantified genomic DNA was taken for Library preparation. Genomic DNA was fragmented and adapter-tag was added using Sure Select QXT Enzyme. Fragmented DNA was cleaned using HighPrep beads. Cleaned adapter tagged fragments were amplified and indexed according to the protocol. The resulting products were done using High Prep beads. The prepared libraries were quantified using Qubit flourometer and validated for quality by running an aliquot on High Sensitivity Bioanalyzer Chip (Agilent). (Refer Figure 1 for Bioanalyzer profiles of amplified product ePCR1)