16S water-column July,2015: replicate 2
Water was collected from near the surface (0-1m depth) from North Shore Channel, and passed on-site in succession through ~1.6 um pore size glass fiber filter for pre-filtrationand and captured on a 0.22 um pore size polycarbonate membrane filter for collecting free-living microbial component. DNA was extracted using phenol:chloroform based extraction, and used for PCR amplification of 16S rRNA gene with primers 27F/534R. PCR products were submitting for sequencing in paired-end format (300bp each). Water samples were collected in duplicates, and this library represents one of the biological replicates.
J2015-16S-B