GSM3703359: Mixture 12: 20% HEK, 80% Jurkat; Homo sapiens; RNA-Seq
mRNA was extracted using oligo(dT) beads (Invitrogen) RNA sequencing libraries were prepared and quantified as described previously in Vincent et al 2015. 1 uL of a 1:25 dilution of ERCC spike-in control (Invitrogen) was added to the mRNA prior to generating barcoded cDNA. At least n=2 technical replicates were performed for each condition.