GSM1908010: ChIP-seq_Mouse_Input; Mus musculus; ChIP-Seq
Fixation: 10 min 1% formaldehyde. Nuclear extracts sonication: Bioruptor 14 time 30s on / 30s off and cleared by centrifugation. Decrosslinking 65°C overnight. DNA purification: minielute (Qiagen) . E2F1 ChIP, samples were precleared with protein A agarose / salmon sperm DNA and chromatin was immunoprecipitated with anti–E2F1 c-20 antibody (santa cruz sc-193). Immunoprecipitated chromatin was washed (low salt, high salt, LiCl and TE buffer), reverse-crosslinked (65°C overnight) and purified with mini elute columns (Qiagen). Qubit fluorometric quantification (Life Technology) was used to quantify DNA. Illumina TruSeq ChIP Sample Prep reagents, according to the provider's protocol