GSM2157791: Wild-type 2 (2); Arabidopsis thaliana; RNA-Seq
Nine-day-old wild-type  seedlings grown on MS plates were harvested and total RNA were extracted using the RNeasy Plus Mini kit (QIAGEN) according to the manufacturer’s instructions. Ribosomal RNA was removed by Ribo-zeroTM Rrna Removal Kit (Epicentre), NEBNext® UltraTM Directional RNA Library Prep Kit for illumina® (NEB) was used to construct the library and sequencing was done on Illumina Hiseq 2500 platform.