BS-Seq of human: Skin/Dermal fibroblast.: Sample bs4.Aff1.K190M.2hr_stress.1
"Human dermal fibroblasts lacking NSUN2 were infected with p-Babe expressing empty vector, wt-NSUN2 or NSUN2-K190M. They were either untreated or treated with sodium arsenite for 2 or 4 hours. Total RNA was extracted using Trizol, treated with TURBO DNase and rRNA depleted using the RiboZero kit. RNA samples were then bisulfite converted using reagents from the Qiagen Epitect kit. Samples were treated with T4 PNK for library preparation using the Illumina TruSeq Small RNA kit."
bs4.Aff1.K190M.2hr_stress.1