GSM2773993: DB7938357_Etv4_Prom_B3_dCas9_mES_situ_rep2; Mus musculus; OTHER
Cells were lysed, digested with DpnII, religated, digested for a second time with either CviQI or BafII, and religated again. This template was purified and amplified with specific viewpoint primers to generate the libraries. Cell lysis and sequential digest followed by ethanol precipitation