GSM2350015: AIDTET_KO2 RNA-Seq; Mus musculus; RNA-Seq
Bone marrow was extracted from femurs, tibiae and iliac crests. After red blood cell lysis, cells were FACS-sorted to isolate Lin- Sca1+ cKit+ (LSK) cells. Total RNA was extracted using AllPrep Kit (Qiagen) Libraries were constructed using the TruSeq Stranded mRNA Library Prep Kit (Illumina) per manufacturer's recommendations