GSM2691104: Pali1_sKO_EB_D4_rep2; Mus musculus; RNA-Seq
RNA was extracted using Rneasy Kit (Qiagen) according to manufacturers instructions. Libraries were prepared using the Truseq Stranded Total RNA library prep kit Ribo-Zero Human/ Mouse/ Rat (Illumina) according to manufacturer’s instructions. Sequencing was performed on an Illumina HiSeq 2500 sequencer using HiSeq v4 chemistry following the manufacturers protocols.