ITS region of deadwood, sample U2b__2014
Amplicons were generated using the primers for ITS1F and ITS4 (Gardes and Bruns, 1993) to characterize fungi (5-CTTGGTCATTTAGAGGAAGTAA-3, 5-TCCTCCGCTTATTGATATGC-3)  Q5 High-Fidelity DNA Polymerase and buffer (New England Biolabs; M0491), hereafter Q5, were used on samples collected in 2014.
U2b__2014ITS