GSM3258529: mla6 + bln1_pool; Hordeum vulgare; Blumeria graminis f. sp. hordei; OTHER
Total RNA was isolated from 0.5 g of each seedling pool using a hot (60°C) phenol / guanidine thiocyanate method (Caldo et al., 2004. Plant Cell 16: 2514-2528. DOI: 10.1105/tpc.104.023382). PARE DNA-sequencing (PARE-seq) libraries were prepared by the Meyers group at the Danforth Center (St. Louis, MO, USA) using the protocol described in [Zhai et al., 2014. Methods 67(1): 84-90. DOI:10.1016/j.ymeth.2013.06.025] and were subjected to single-end sequencing (50-bp reads) using the Illumina Hi-Seq2500 Sequencing System. The five libraries were barcoded separately and run on a single flow cell lane.