GSM1974457: rnaseq replicate 2; Saccharomyces cerevisiae; RNA-Seq
Total RNA was extracted from clarified lysate and depleted of ribosomal RNAs using RiboZero.  Remaining RNAs were fragments and size selected. Collected cells were resuspended in lysis buffer and lysed by vortexing with glass beads. RNAs were ligated to a preadenylated DNA linker and reverse transcribed.  cDNAs were gel purified, circularized, and PCR amplified.