GSM3762825: HUDEP2_D3_H3K27ac_HiChIP; Homo sapiens; OTHER
HiChIP samples on 10 million cells each sample using H3K27ac antibody were prepared according to the published protocol (Fang et al., 2016; Mumbach et al., 2016) with the following modification. After proximity ligation, the nuclear lysates were sonicated using a Branson 250 Sonifier (microtip, 100% duty, 20%outpt, 12x10s intervals). 8ug of H3K27ac antibodies were used for each sample. The reverse cross-linked ChIP DNA were purified using DNA clean & concentrator-5 (Zymo Research) and quantified using Qubit (Life Technologies). The samples were end repaired, dATP labeled and adaptor ligated using NEBNext ultraII DNA library prep kit, followed by streptavidin pull-down and PCR amplification of library. 300-1000bp of PCR products were size selected using E-gel