GSM4083915: FGF2 - pPPR explants Input; Gallus gallus; ChIP-Seq
Explants were dissociated in 0.5ml of Nuclei Extraction Buffer, homogenized and cross-linked using 1% formaldehyde for 9 min at room temprature, 0.125M glycine final concentration was used to stop fixation. Total of 100 tissues were pulled together and sonicated. Chromatin was amplified with a step of linear amplification (15 cycles) following the protocol described in (Adli and Bernstein, 2011). Libraries were prepared according to nano-ChIP-seq (Adli and Bernstein, 2011) with the exception that only 12 cycles were used in the PCR amplification, following Illumina's instructions.