WGS of Candida albicans Wild Type cell wall mutants (new)
A single colony was picked from a YPD-agar plate and grown overnight in 10 ml of YPD at 37 degrees Celsius under orbital shaking at 150 rpm. 1 ml of overnight culture was harvested by centrifugation at 3500 rpm for 5 minutes. Genomic DNA was extracted using the phenol-chloroform-isoamylalcohol method as described in G. Rancati et al., Cell 2008 [Pubmed ID: 19041751]. Libraries were prepared using Illumina TruSeq® DNA PCR-Free library prep kit, enriching for DNA fragments of 550bp in length according to manufacturer’s instructions.
WYU007