GSM3178829: S9; Temora longicornis; RNA-Seq
Total RNA was extracted from adult copepods using the MasterPureTM RNA Purification Kit (Epicentre®, tebu-bio NV), following the manufacturer's protocol. An Illumina mRNA sequencing library was made from one µg total RNA of each sample using the TruSeq Stranded mRNA Sample Prep Kit (Illumina, San Diego, USA). The size distribution, purity (absence of free adaptors) and quantity of the resulting libraries were measured using a High Sensitivity DNA chip (Agilent Technologies, Santa Clara, CA, US). The libraries were equimolarly pooled and sequenced in three lanes of an Illumina HiSeq 3000 flow cell, generating paired-end 2x150bp reads.