GSM1808724: RRBS_4_Mouse_ProB_TET2KO; Mus musculus; Bisulfite-Seq
DNA was isolated from isolated B-cells and incubated in lysis buffer (25  mM Tris-HCl (pH  8), 2  mM EDTA, 0.2% SDS, 200  mM NaCl) supplemented with 300 μg/ml proteinase K (Roche) followed by phenol:chloroform extraction and ethanol precipitation. RRBS libraries were prepared as described (Boyle, P. et al. 2012)