TD-seq T.vaginalis
Transposon Display sequencing (TD-seq) libraries, a sequencing method that selectively amplifies TE families and their adjacent regions in order to characterize transposable elements (TE) insertion variation in different isolates were prepared using NlaIII digested DNA. A sequencing adapter with the standard Illumina multiplexing read index with barcodes was used to track individual isolates. All libraries were selectively amplified using a specific Illumina adapter primer and hybrid of adapter sequence and Maverick TE specific primer 5 GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT GGTTTYGTAAAGTGTCAAAT 3, where Y represents C or T nucleotide.
SD2_MV_Library