Pea aphid clone Lotus corniculatus clone 101 reared on Vicia faba
Typically the RNA of 20 heads from wingless adult aphids per clone/test plant combination was extracted and pooled for RNA-seq analysis. RNA extractions were performed using the Macherey Nagel NucleoSpin RNA II kit, following the manufacturer’s instructions. Barcoded RNAseq library was prepared using Illumina TruSeq RNA Sample Prep Kit v2 using 1 μg of total RNA input and 10 PCR cycles, as per the manufacturer's recommendations. Final library were quantified by qPCR and combined into 6-plex pools prior to sequencing
101V