GSM2775134: HCT116_(-MET)_H3K4me3_ChIPseq_Rep2; Homo sapiens; ChIP-Seq
Cross-linked chromatin was collected and flash frozen before sonication with the Covaris M2. Spike-in Drosophila chromatin and spike-in antibody for Drosophila H2Av (Active Motif cat#61686) was mixed with chromatin from the HCT116 cells before the chromatin IP step with a fixed ratio (2:1, Drosophila:HCT116 chromatin) as a reference. Lysates were clarified and protein-DNA complexes were immunoprecipitated with H3K4me3 antibody. Libraries were prepared according to standard Illumina protocols.