GSM1974225: H3K27me3_stage11_amanitin_rep2; Xenopus tropicalis; ChIP-Seq
Protein-DNA complexes were isolated with antibody from whole embryo lysates that were fixed, sonicated and yolk depleted. Total RNA was subjected to depletion of ribosomal RNA (rRNA) using Ribozero Epicenter low input kit. Libraries were prepared with the Kapa Hyper Prep kit (Kapa Biosystems), and sequencing was done on the Illumina HiSeq2000 platform.