Illumina Genome Analyzer II sequencing; RBM20 orchestrates cardiac pre-mRNA
RBM20 orchestrates cardiac pre-mRNA
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Human embryonic kidney (HEK) 293 cells with tetracycline-inducible expression of FLAG/HA-tagged RBM20 have been described previously (5). For PAR-CLIP, we essentially followed the protocol as described (13). The libraries were sequenced on an Illumina Genome Analyzer GAII using the 50 bp single read protocol. 4 ug of total RNA was used for library preparation with the TruSeq RNA Sample Preparation Kit (Illumina).