Whole genome sequencing of urinary cell-free DNA from kidney transplant patient: Sample S1
Short cell-free DNA molecules were extracted from urine supernatants and ligated to a biotinylated adapter. Molecules were then bound to magnetic beads and end repair and ligation to a double stranded sequencing primer were performed. DNA libraries were released from bead via denaturation. Libraries were then amplified and indexed using PCR.
S1