GSM2232849: N. stephensi replicate 3; Neotoma stephensi; RNA-Seq
We collected a small section of the fornix ventricularis of the stomach (or the fundus of the stomach), which was immediately rinsed in ice-cold physiological saline buffer and placed in RNAlater solution. After 24 hours, the RNAlater solution was removed and tissues were frozen at -80°C. Total RNA from each animal was isolated from frozen tissue samples using Qiagen RNeasy kit according to manufacturer instructions (Valencia, CA). RNA libraries were prepared for sequencing using standard Illumina protocols.