GSM3104179: 1_DAT_Control_4 [RNA-seq]; Triticum aestivum; RNA-Seq
Leaf tissue was collected from all heat-stressed and control individual plants immediately at the end of the stress period, i.e., time point zero day after treatment (0 DAT), and at one and four days after treatment (1 and 4 DAT). Total RNA was isolated from all 432 leaf tissue samples using TRI-reagent (Ambion, Naugatuck, CT) as previously described (Ragupathy et al., 2016; Scientific Reports, 6, 39373). mRNA enrichment, library construction and sequencing were performed by the McGill University and Genome Quebec Innovation Centre (Montreal, QC, Canada). The clustering was done on an Illumina cBot and the flowcell was run on a HiSeq 4000 for 2x100 cycles (paired-end mode) following the manufacturer's instructions.