GSM3103009: Mouse Hyaloid P5; Mus musculus; RNA-Seq
Total RNA was prepared from lysate of two hyaloid tissues at P5 in Tri Reagent (ThermoFischer Scientific) and purified by the RNeasy Micro column (Qiagen). RNA quality was verified by Agilent Bioanalyzer (Agilent Technologies). The Ovation RNA-Seq System v2 (NuGEN) was used for RNA amplification and double-stranded cDNA construction. DNA concentration was measured by Qubit (ThermoFisher Scientific), while the DNA length was determined by the DNA 1000 Chip (Agilent Technologies). DNA library templates were then created by the Nextera XT DNA Sample Preparation Kit (Illumina) and sequenced for the paired-end sequencing with 25-30 million reads by Illumina HiSeq2500 (Illumina).