GSM2691581: Cells deleted for MftR grown with urate_KU_2; Burkholderia thailandensis; RNA-Seq
Cells were collected by centrifugation followed by twice washing with chilled DEPC-treated water. Total RNA was isolated from three replicates of each condition using illustra RNAspin Mini Isolation kit (GE Healthcare). All samples were rRNA depleted using Ribo-Zero rRNA removal kit (Bacterial (Illumina)). The strand specific libraries were prepared using TruSeq stranded RNA sample kit (Illumina).