GSM2501250: IGR39_t0_R3; Homo sapiens; RNA-Seq
ChIP-seq: 3x-15cm dishes of 501mel cells were fixed in 0.4% PFA then cheared with a Covaris S220 for 15 minutes (160 W peak incident power, 5% duty cycle and 200 cycles per burst). The sonicated chromatin were precleared and ChIP was performed using 80ug of the indicated antivodies. Purification of the ChIP-DNA was done using QIAGEN PCR clean-up column RNA-seq: RNA-samples were extracted using QIAGEN RNeasy kit ChIP-seq: Libraries were prepared using recommended Illumina protocols RNA-seq: Library prep using QuantSeq Forward kit (LEXOGEN #015.96), using 500 ng starting material