GSM3177875: input DNA_cort; Mus musculus; OTHER
Cells were formaldehyde fixed and chromatin lysates were prepared by sonication (4x10s in Bioruptor) and benzonase-treatment (16U, 15 mins, 25oC). ChIP-nexus was performed as in published protocol (He et al., 2015). Briefly, samples were end-repaired, dA-tailed, adapters were ligated, 5' overhangs were filled in, 5' ends trimmed, lambda-exonuclease treated, RecJ exonuclese digested, DNA was eluted, DNA was circularized, oligos annealed, BamHI digested and enriched by PCR-amplification for 18 cycles. PCR-amplification introduced bar-coded using NEBNext primers for Illumina  indexes 1 to 18. Input samples were prepared with NEBNext Ultra II DNA Library Prep with NEBNext Multiplex Oligos for Illumina (set 1 and 2, indexes 1 to 18). Sequencing was performed with NextSeq 500 Hi-Output Kit v2 (75 Cycles).