Illumina HiSeq 2500 sequencing; OPLAH prevents accumulation of cardiotoxic 5-oxoproline after ischemic injury and improves cardiac function
OPLAH prevents accumulation of cardiotoxic 5-oxoproline after ischemic injury and improves cardiac function
EB12_3_s
A total of four stages of murine cardiac development (embryonic day 12, embryonic day 18, post-partum day 2, 4 weeks) were utilized in this study. Female mice at day 12 and 18 of pregnancy were sacrificed followed by the excision of the embryos. For embryonic day 12 the whole heart was removed for each embryo. At embryonic day 18, the left ventricle was excised from the embryos. The left ventricle of post-partum day 2 pups were removed following decapitation. 4 week old mice were sacrificed after which the left ventricle was isolated. We also included 20 week old mice which had undergone ischemia/reperfusion injury or sham treatment.   Total RNA was isolated by the Trizol RNA isolation protocol. Libraries were generated strictly NEXTflex™ Illumina RNA-Seq Library Prep v2 kit recommendations (Bioo Life Science).