GSM1422513: D75; Notamacropus eugenii; miRNA-Seq
the milk samples were thawed on ice and centrifuged at 5000xg to isolate the fat and cells. Skim milk was centrifuged at 17,000Xg for 20 min to isolate any remaining cells and fat. miRNAs were isolated from skim milk by using commercially available miRNA isolation Kit (mirVana™ miRNA Isolation Kit, Ambion) and the isolation was performed as instructed in the manufacturer's manual. The miRNA was eluted with 100µl of elution solution provided in the kit. BGI small RNA-seq (40 nucleotide cut-off)