Transcriptome sequencing of a fungal plant pathogen, Macrophomina phaseolina causing stem rot in jute under controlled in vitro conditions
Total RNA extracted from the fungal hyphae of Macrophomina phaseolina, isolate CJMR17, from in vitro Hoagland culture. The high-quality RNA were subjected to ds cDNA conversion using SMARTer ultra-low input RNA for Illumina Sequencing followed by Covaris shearing and end-repair of overhangs resulting from the shearing. The paired-end cDNA sequencing libraries were prepared using the Illumina TruSeq stranded mRNA Library Preparation Kit. Library quantification and quality was estimated using DNA High Sensitivity Assay Kit in Bioanalyzer 2100. The library fragment size range was chosen between 650 to 670 bp. Finally, the libraries were sequenced using 2 X 150 Paired End (PE) chemistry to generate 5-6 GB data/sample.
MPC_TRA1