GSM4570156: STR_YAYC_4; Mus musculus; RNA-Seq
Total RNAs were prepared from the sorted cells by RNeasy Micro Kit (Qiagen), and analyzed by Agilent Bionalyzer. Their RNA integrity numbers (RINs) were 6.7~9.0 (average is 8.2) and obvious RNA degradation was not observed. Because the RNA concentrations were low (~2 ng / μL), we used Clontech SMARTer Ultra Low Input RNA Kit for Sequencing (v3) for efficient amplification of cDNA after reverse transcription according to the manufacture's protocol. Libraries for sequencing were prepared by Nextera XT Sample Prep Kit, and sequenced by an Illumina HiSeq 2500 system (single read; 50 cycles; rapid run mode).