GSM2587327: iExo [ncRNA-Seq]; Homo sapiens; ncRNA-Seq
Exosme was extracted from each culture medium of the ultrasound treated HDF cell by miRCURYTM Exosome isolation kit. Total RNA of exosome was extracted by miRCURYTM RNA isolation kit (Exiqon. Inc.). The isolated RNA from exosome and cells were quantificated by Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA). The exosomal RNAs were performed to library using NEBNext Multiplex Small RNA library prep kit (New England BioLabs, MA, USA)