GSM1543978: H3K4Me2_DMSO; Homo sapiens; ChIP-Seq
ChIP was performed using a HighCell# ChIP kit (Diagenode, Liege, Belgium) according to the manufacturer’s instructions. Prior to ChIPseq, DNA was purified with an iPure kit (Diagenode), according to manufacturer’s instructions. To prepare samples for sequencing on the Illumina HiSeq 2500 (Illumina, San Diego, CA), a Microplex Library Preparation Kit (Diagenode) was used to generate libraries from 1ng ChIP DNA. Libraries were then size selected (200-800 base pairs) by adding 0.55x volume of AMPure beads (Beckman Coulter, Pasadena, CA) to the sample, followed by 0.3x volume of AMPure beads to the supernatant. The supernatant was then discarded and the beads washed with 70% ethanol before drying and elution of the size selected library. Library quantitation was performed by Q-PCR using a Kapa Library Quantification Kit (Kapa Biosystems, Woburn, MA). Next, 15pM of the library was used for on board cluster generation in the Rapid Mode of a HiSeq 2500 (Illumina) and then paired end 75 or 101 base pair sequencing was performed using a TruSeq Rapid SBS Kit (Illumina).