GSM2346471: E12.5_Heart_ATAC_rep2; Mus musculus; OTHER
Isolated cardiomyocytes from mouse E12.5 ventricles were immediately centrifuged at 500 x g, 4 degrees, 10 minutes with nonionic detergent. Nuclei pellets were resuspend in a Tn5 transposase reaction mix (Nextera) and incubated at 37 degrees for 30 minutes. Tn5-cleaved euchromatin was purified using a Qiagen MinElute PCR Purification kit. Amplification of transposed DNA fragments was performed using Illumina adapters and NEBNext High-Fidelity 2x PCR Master Mix (NEB). Appropriate cycle number was determined after five initial PCR cycles via qRT-PCR using SYBER Green I (Life Technologies). Libraries were purified using a Qiagen MinElute PCR Purification kit.