GSM2691576: Wild-type grown with urate_WU_3; Burkholderia thailandensis; RNA-Seq
Cells were collected by centrifugation followed by twice washing with chilled DEPC-treated water. Total RNA was isolated from three replicates of each condition using illustra RNAspin Mini Isolation kit (GE Healthcare). All samples were rRNA depleted using Ribo-Zero rRNA removal kit (Bacterial (Illumina)). The strand specific libraries were prepared using TruSeq stranded RNA sample kit (Illumina).