GSM2235678: CCLG14-464; Homo sapiens; OTHER
RNA was extracted from tumor using an RNeasy Fibrous Tissue kit (Qiagen). Genomic DNA was extracted from tumor using a DNeasy Blood & Tissue kit (Qiagen). Genomic DNA from blood was obtained from the Children’s Cancer and Leukaemia Group Tissue Bank, Newcastle, UK. RNA libraries were prepared for sequencing using standard Illumina protocols For whole-exome sequencing, targets were captured using SureSelect (Agilent). Samples were prepared and enriched according to the manufacturer's instructions. The concentration of each library was determined using a QPCR NGS Library Quantification Kit (G4880A, Agilent).