GSM3259556: C. jejuni PT14 spiral stationary phase replicate 1; Campylobacter jejuni; RNA-Seq
Total RNA was isolated from the cell morphotypes using the Trizol Max Bacterial Isolation kit (Invitrogen) and purified using an RNeasy Mini kit (Qiagen) and the integrity was measured using a Bioanalyser 2100 (Agilent Technologies). Ribosomal RNAs were depleted from the total RNAs using the Ribo-Zero™ Magnetic Kit for bacteria (Illumina) and used to prepare cDNA libraries using the NEBNext Ultra directional Prep Kit.