GSM4083364: MS CP 5; Homo sapiens; RNA-Seq
Up to 30 mg of tissue was disrupted from the whole lateral CP by cutting slices approximately 10 µm thick using a cryotome at -20oC. Tissue was homogenized with a POLYTRON PT 1200 (Kinematica). Total RNA from the CP was extracted with the RNeasy mini kit (Qiagen, 74104), following the manufacturer's instructions (using 600 µL of lysis buffer). RNA libraries were prepared according to the manufacturer's instructions using TruSeq stranded mRNA Library Prep Kit including an rRNA depletion step (RiboMinus, ThermoFisher).