1C+aAM
alpha-Amanitin treated 1-cell (1C) embryos
One-cell embryos were transferred to 20 ul drop of M16 medium containing 10 ug/ml alpha-amanitin (Sigma A2263) three hours after insemination. Total RNA was prepared from about 2300 first mitosis-arrested embryos using ISOGEN II (NIPPON GENE). For RNA-seq library construction, we performed TruSeq Standard Total RNA Sample Prep Kit (Illumine, San Diego, CA #RS-122-2201 and 2202) and prepared 600 ng RNAs.