GSM3703258: xrn1 biological replicate 2 (total RNA-seq); Naumovozyma castellii; ssRNA-seq
Total RNA was extracted using standard hot-phenol procedure. For each sample, 1 µg of total RNA was mixed with 2 µl of 1:100 diluted ERCC RNA spike-in (Life Technologies), then ribosomal (r)RNAs were depleted using the RiboMinus Eukaryote v2 Kit (Life Technologies). Total RNA-Seq libraries were constructed from 50 ng of rRNA-depleted RNA using the TruSeq Stranded mRNA Sample Preparation Kit (Illumina). Paired-end sequencing (2 x 50 nt) was performed on a HiSeq2500 system (Illumina).