GSM4009078: The effect of ZBTB11 overexpression on iPS derived cardiomyocytes; Homo sapiens; RNA-Seq
Total RNA was extracted from cells 7 days after transduction using standard TriZol extraction. Samples were barcoded with CEL-seq primers during a reverse transcription and pooled after second strand synthesis. The resulting cDNA was amplified with an overnight In vitro transcription reaction. From this amplified RNA, sequencing libraries were prepared with Illumina Truseq small RNA primers.