GSM3701971: Crypts_VEMD4; Mus musculus; RNA-Seq
Crypts were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. RNA libraries(WT, VEDM2, VEMD3, VEMD4, VEMD6, VEMD8) were prepared for sequencing using standard BGI-500 protocols. Besides this, purified total RNA from ISC cells isolated from Lgr5–EGFP–ires–CreERT2-mice was quantified by Qubit(invitrogen) and analyzed by an Agilent Bioanalyzer to evaluate RNA quality. rRNA-depleted was used to generate the cDNA library. cDNA library construction and sequencing were prepared for using illumina Hiseq X-TEN protocol.