GSM4082543: 231_H3K27me3_E111D_boy_ICSI-ET; Homo sapiens; ChIP-Seq
Genomic DNA (gDNA) was extracted from stored whole blood samples using the QIAmp DNA Blood Mini Kit (Qiagen, cat.: 51106), according to manufacturer's instruction and stored in -80 ℃ if not used immediately. Briefly, isolated nuclei were sonicated into fragments of an average of 200–500 bp using an M220 Focused-ultrasonicator (Covaris) with the following parameters: burst, 200; cycle, 20%; and intensity, 8. After centrifugation (13000 rpm for 10 min at 4°C), sheared chromatin in supernatant was precleared by incubating the samples with protein A Dynabeads (Invitrogen, cat.: 10002D) at 4°C for 1 h with rotation.