de novo  Ocimum sanctum Genome sequencing
Library for sequencing was constructed according to the Roche Rapid Library Preparation Method Manual (GS FLX+ Series – XL+, May 2011). Briefly, ~1 microgram of genomic DNA was made up to 100 ul with TE buffer and fragmented using a nebulizer. The resulting fragmented DNA was cleaned up using QIAGEN Minelute PCR purification kit. Subsequently, DNA was subjected to a series of enzymatic reactions that repair frayed ends, and ligates adaptors. After ligation, small fragments were removed using Agencourt Ampure SPRI beads. The prepared library was validated for quality by running an aliquot on High Sensitivity Bioanalyzer Chip (Agilent). (Refer Figure 3 for Bioanalyzer profiles of amplified product ePCR1)The library shows a peak at the range of 1.4 – 1.8 kb. The library is suitable for sequencing on 454 platform.
CIMAP_454_GS_FLX_SIngle_ended