A. argentata Total Silk Gland RNAseq
An adult female Argiope argentata was collected in San Diego County, CA, USA. The total set of silk glands was dissected. Total RNA was extracted from tissue homogenized in TRIzol (Invitrogen, Carlsbad, CA, USA) and purified with an RNeasy Mini kit (Qiagen, Valencia, CA, USA). Carryover genomic DNA was removed with Turbo DNase (Life Technologies, Grand Island, NY, USA). Each RNA extraction was quantified with a Nanodrop ND-1000 (ThermoFisher Scientific, Waltham, MA, USA) and examined for integrity with a Bioanalyzer (Agilent 2100, Santa Clara, CA, USA). Samples were stored at -80°C.   The total RNA extracted was made into an RNAseq library using the TruSeq RNA Sample Prep Kit v1 (Illumina, San Diego, CA, USA). Total RNA from the cephalothorax tissue replicate with the higher RNA yield (Cep_R2) and the two tubuliform silk gland replicates (Tub_R1 and _R2) were made into three RNAseq libraries with the TruSeq RNA Sample Prep Kit v2 (Illumina) by the Johns Hopkins University School of Medicine Deep Sequencing and Microarray Core.   Library was sequenced on a HiSeq2500 platform (Illumina) at the University of California, Riverside Institute for Integrative Genome Biology. The library was paired-end sequenced for 100 cycles.
A. argentata Total Silk Gland RNAseq