GSM4008817: Direct-zol Kit isolated 20min heat Rep2; Saccharomyces cerevisiae; RNA-Seq
Each technical replicate had RNA extracted by one of three methods: 1) hot phenol from Gasch, Methods in Enzymology, 2002, followed by cleanup with a Qiagen RNeasy column including optional on-column DNase digestion, 2) Qiagen RNeasy kit including optional on-column DNase digestion; 3) Zymo research Direct-zol kit RNA-seq libraries were prepared from polyA-enriched RNA using the KAPA Biosystems mRNA HyperPrep Kit (KK8581) and KAPA Single-Indexed Adapter Set A+B (KK8700). All libraries were constructed in a single batch through an automated Eppendorf epMotion 5075 liquid handling robot