16SrRNA-TRIP_phenanthrene
The TRIP culture was grown with phenanthrene as carbon source and sulfate as electron acceptor until exponential phase. Cells were harvested by centrifugation (20 min, 6000 x g, 4 C) and DNA was extracted using the DNeasyTM Blood and Tissue Kit (Qiagen). DNA was used to amplify the V3-V4 region of the 16S rRNA gene for library construction. PCR products were converted into blunt ends by using T4 DNA polymerase Klenow Fragment and T4 Polynucleotide Kinase previous to adaptor addition. After quality control, libraries were sequenced (PE 250 on HiSeq 2500).
TRIP_phenanthrene_2017