GSM1424408: MNase-seq from WT yeast; Saccharomyces cerevisiae; MNase-Seq
After collection, the cells were cross-linked with formaldehyde, the cell wall was digested with Zymolyase, and the chromatin was digested with a titration of MNase. The resulting DNA was electrophoresed on an agarose gel, and an ideal digestion in which most of the genome (>80%) was digested into a mono-nucleosome was chosen (see Rando, 2010 in citation references). The mononucleosomal DNA was then isolated, end-repaired for library preparation. MNase- seq libraries were constructed with NEBNext® UltraTM DNA Library Prep Kit for Illumina®. The DNA was size selected and amplified by PCR using the adaptor primers. The amplified DNA was then purified and used for cluster generation and sequencing on the Illumina® HiSeq system.