GSM4084070: HYNLE+HC3tx_mms7661+liver; Hynobius leechii; RNA-Seq
The extraction of total RNA was done by lysing the embryos in Trizol reagent (Invitrogen), and purified with Direct-Zol RNA purification kit (Zymo) RNA sequencing library was constructed according to manufacturer's instructions (Illumina, San Diego, CA, USA) after polyA enrichment.