GSM4633521: Pachon_H3K4me3_1ug_ip_1889; Astyanax mexicanus; ChIP-Seq
Adult livers were dissected and fixed in 1% formaldehyde for 15 min. Chromatin was prepared using MAGnify Low Cell ChIP-Seq Kit (Thermo Fischer) and ChIP was done for H3K4me3 (Millipore, # 07-473), H3K27ac (Abcam, # ab4729), H3K27me3 (Abcam, # ab195477). 200,000 cells and 1ug of antibody was used for each sample. Libraries were prepared using the KAPA HTP Library Prep Kit for Illumina and Bioo Scientific NEXTflex DNA barcodes. The resulting libraries were purified using the Agencourt AMPure XP system (Beckman Coulter) then quantified using a Bioanalyzer (Agilent Technologies) and a Qubit fluorometer (Life Technologies).  Post amplification size selection was performed on all libraries using a Pippin Prep (Sage Science). Libraries were re-quantified, normalized, pooled and sequenced on Illumina Illumina HiSeq 2500 instrument as 50-bp single read.