GSM1542422: C_albicans_R2_with_iron; Candida albicans; MNase-Seq
Briefly, in vivo chromatin structure was fixed through treatment with formaldehyde, isolated using mechanical digestion via bead-beating, and mono-nucleosomal DNA was isolated for sequencing using a combination of MNase digestion followed by phenol:chloroform-based DNA precipitation. MNase digestions were closely matched to enrich for a near 100% mono-nucleosomal DNA population to limit the influence that DNA fragment size-selection has on downstream signal comparisons between distinct MNase-seq datasets MNase-seq libraries were generated using standard Illumina ChIP-seq procedures