GSM1754761: lag_e_chip; Caenorhabditis elegans; ChIP-Seq
Immunocomplexes, from each sample, were collected using 10 µg of M2 Flag antibody (F1804 Sigma) or 10 µg of 9E10 Myc antibody (Sigma M4439) overnight at 4 °C.  Then each sample was incubated for 1-2hr at 4 °C with 75 µL Dynabeads-Protein G (Novex 10003D).  Lysate samples and immunoprecipated protein samples were analyzed by immunoblot analysis using standard protocols. ChIP-Seq DNA libraries were prepared using standard protocols and assessed for integrity using the Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA).  DNA sequencing was performed on four lanes of an Illumina HiSeq2000 at the University of Wisconsin-Madison Biotechnology Center (Madison, WI).