Illumina HiSeq 2000 sequencing; Posttranscriptional mRNA expression changes in stress
Posttranscriptional mRNA expression changes in stress
nuc_nucRNA-Aniso-RII
Cells were seeded 48 hours prior to stress treatment. Cells were subsequently treated with 5 ??g/ml Anisomycin for 3 hours and harvested in PBS. Total RNA was extracted using Trizol LS. Cells were fractionated and nuclear RNA was extracted using the Paris Kit (Life technologies) according to the manufacturer's instructions. mRNA-Seq libraries (from polyA+ cytoplasmic RNA) were created according to the standard Illumina TruSeq protocol.