GSM1642945: RNAseq_TTX_scramble_rep1; Mus musculus; RNA-Seq
Total RNA was poly-A tail selected and then heat fragmented. The fragmented RNA was reverse transcribed and the second strand was synthesized to make double stranded DNA. After end repair and 3’ adenylation, adapters for multiplexing were ligated to the end of double stranded DNA fragments. The ligation products were amplified and purified to generate illumina compatible libraries. Illumina Truseq RNA sample prep