GSM2235642: AB-biological rep2; Streptococcus mutans; RNA-Seq
Collected cells were treated withRNAprotect reagent (Qiagen) Total RNA was isolated and purified by RNeasy Mini Kits (Qiagen) with on-column digestion by RNase-free DNase I mRNA was enriched by using the MICROBExpress™ Bacterial mRNA Enrichment Kit (Ambion) cDNA libraries were constructed from the enriched mRNA samples by using NEBNext Ultra directional RNA library prep kit for Illumina and NEBNext multiplex oligonucleotides for Illumina (New England BioLabs),