RNA-seq of Boreogadus saida: muscle
Total RNA was extracted from sample using a MasterPure™ RNA Purification Kit (Epicentre, Madison, WI, USA) following manufacturer’s protocol and quantified using a Quant-iT RNA Assay Kit (Invitrogen, Carlsbad, CA USA). Libraries were generated from high-quality RNA using the TruSeq® stranded mRNA library preparation kit following the low sample protocol (Illumina, San Diego, CA USA).  Concentrations of the  final library were quantified using Quant-iT RNA Assay Kit and sequenced on  Illumina MiSeq Desktop Sequencer [2 × 150 bp (base pair) read-length configuration] on a run consisting of 12 total libraries.
BOSA_A6-20_1Muscle