GSM2159740: A3; Bos taurus; miRNA-Seq
Total RNA were isolated from mouse tissue biopsies or from the samples obtained after AGO2 co-immunoprecipitation using the RNA NOW kit (Ozyme), with overnight precipitation so as to guarantee a maximum yield of small RNA. Bovine total RNA were prepared from about 50 mg mammary tissue using the Nucleospin® miRNA isolation kit (Macherey-Nagel Inc.) according to the manufacturer’s instructions. Both mouse and bovine samples were precipitated using sodium acetate 3M, 96% ethanol and 5 mg/mL glycogen. Libraries were prepared using the Illumina small RNA kit (Illumina) from GATC Biotech Company, according to the manufacturer’s instructions.