GSM2507612: HDF85_quiescent_day7; Homo sapiens; miRNA-Seq
Total RNA was isolated with QIAzol Reagent (Qiagen) and RNA was automatically extracted by QIAcube technology based on miRNeasy Mini kit (Qiagen, 217004). RNA concentration and quality was analysed with a Bioanalyzer 2100 using the RNA 6000 Nano Kit (Agilent Technologies). Intracellular small RNA cDNA library for Illumina Sequencing was synthesized according to the manual provided by NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible) (NEB, E7330S). From initially 1 µg of total RNA, small RNA fragments of approximately 18 – 36 nucleotides were purified on a 10% TBE gel, quantified by 2100 Bioanalyzer  (Agilent) and equimolar amounts were pooled and sent to Exiqon (Denmark) for Illumina RNA-Seq.