GSM4633805: day 100 gut organoid; Homo sapiens; RNA-Seq
Multilineage and gut organoids at day 100 of culture were washed in PBS and dissociated by successive 20 min incubations in 0.25% trypsin at 37oC (maximum of 5 incubations) until complete dissociation to single cells. 10,000 cells in PBS supplemented with 0.08% FBS were prepared for sequencing by droplet encapsulation using the Chromium Controller and library preparation with the 10x Genomics Single Cell 3' v2 Library and Gel Bead Kit (10x Genomics). Chromium Controller and library preparation with the 10x Genomics Single Cell 3' v2 Library and Gel Bead Kit (10x Genomics). Libraries were sequenced using the NovaSeq S4 (Illumina) to a minimum depth of 50,000 mean reads per cell.