GSM2507446: EHCont_RNAseq_rep1; Mus musculus; RNA-Seq
Cells were sorted and total RNA was isolated from them using the RNeasy-Micro Kit (Qiagen). The total RNA was then processed for deep sequencing using SMARTer Ultra Low Input RNA kit  for Sequencing-v3 (Clontech) following the manufacturer’s protocol. Liibraies were prepared fromt the sonicated DNA using Low Input Library Prep Kit (Clontech) following the manufacturer’s protocol. cDNA libraries were sequenced with a HiSeq 2500 sequencer (Illumina).