GSM2691839: TGF-beta1-treated Smad3-knockout bone marrow-derived macrophage [S3KO-MMT1]; Mus musculus; RNA-Seq
Total RNA from a single-cell suspension was extracted by RNeasy Plus mini kit (Qiagen), and then prepared the cDNA following the SMART-seq2 protocol according to manufacturer’s instruction. A total of 2 ng amplified cDNA products purified by 1 x Agencourt AMPure XP beads (Beckman Coulter) were subjected for library construction by TruePrepTM Mini DNA Sample Prep Kit (Vazyme Biotech).