Inverse toeprints of ermAL1, ermBL, ermCL, ermDL (all in the presence of erythromycin), secM and tnaC (in the presence of tryptophan) wild-type sequences
Illumina sequencing adapter were added to the ds cDNA obtained after inverse toeprinting by PCR. PCR products were then purified using a PCR purification kit, checked for quality and quantity with BioAnalyzer 2100 using the DNA1000 kit.
Wild-types