Illumina HiSeq 2000 paired end sequencing; Establishment of Porcine and Human Expanded Potential Stem Cells (Single-Cell RNA-seq)
Establishment of Porcine and Human Expanded Potential Stem Cells (Single-Cell RNA-seq)
pEPSC_plate1_B11_p
The C5 hiPSC was reprogrammed from the somatic cells derived from post-mortem human fetal neural tissue which was acquired at the time of surgical termination of pregnancy in the day surgery unit of Addenbrookes Hospital, Cambridge. Full informed consent was obtained from participating mothers. All generated lines are unlinked anonymous.. All ​related procedures and experiments were approved by the hospital and Sanger institute. M10 cells were derived from pig fetus fibroblast by reprogramming. Those pig fetus fibroblast cells were derived from a 28-day normal healthy male pig fetus skin. K3 cells were derived from 5-day preimplantation embryos, again, healthy male. All the related embryos and fetus experiments were approved by the Niedersaechsisches Landesamt fuer Verbraucherschutz und Lebensmittelsicherheit, LAVES, Oldenburg Germany. Abbreviations used: PFF means the pig fetus fibroblast. Emb means the origin is 5-day embryos. Parth means parthenogenetic embryos, PGCLC means the primordial germ cell-like cells differentiated from K3 cells.  Cells were sorted into 96 well plate with 0.4% Triton X-100, and cDNA was generated using standard SMART-seq2 protocol. No extraction for nuclei acid. ~0.3 ng cDNA was used to construct sequencing libraries using Illumina Nextera XT Library Preparation Kit according to the manufacturer's protocol.