GSM1971045: RNA_Std_d7; Mus musculus; RNA-Seq
Total RNA was extracted using Trizol reagent following the manufactorer's instructions and treated with DnaseI RNA-seq libraries were generated by BGI following their standard procedure: mRNAs is enriched by using the oligo (dT) magnetic beads. By using the fragmentation buffer, the mRNA is fragmented into short fragments (about 200 bp), then the first strand cDNA is synthesized by random hexamer-primer using the mRNA fragments as templates. Buffer, dNTPs, RNase H and DNA polymerase I are added to synthesize the second strand. The double strand cDNA is purified with QiaQuick PCR extraction kit and washed with EB buffer for end repair and poly (A) addition. Finally, sequencing adapters are ligated to the fragments. The fragments are purified by Agarose gel electrophoresis and enriched by PCR amplification.