GSM1908223: Hhex+/fl tumor rep 2; Mus musculus; RNA-Seq
RNA was extracted from sorted LSKs, GFP+ LICs and GFP+ leukemia cells using the RNeasy Kit (Qiagen, Valencia CA). mRNA reverse transcription and cDNA libraries were prepared using the TruSeq RNA Sample preparation kit (Illumina) following the manufacturer’s instructions.