High throughput sequencing reads from cross linking and immunoprecipitation HITS-CLIP of Saccharomyces cerevisiae Puf5p
High throughput sequencing reads from cross linking and immunoprecipitation HITS-CLIP of Saccharomyces cerevisiae Puf5p. After UV irradiation and cell lysis, extracts were affinity purfied using calmodulin resin followed by IgG beads.  RNA-protein complexes were resolved on an SDS-PAGE gel, transferred to a nitrocellulose membrane and finally the correct molecular weight complexes were excised from the membrane.  Illumina adapters were ligated onto purified RNA and PCR amplified. Illumina sequencing/flow cell adapters were added during second round of PCR. Library was purified by agarose gel extraction.