GSM4007701: m1A Normoxia input - rep1; Homo sapiens; OTHER
Total RNA was isolated from normoxia or hypoxia HTR8 cell lines. Polyadenylated RNA was further enriched from total RNA using a Dynabeads mRNA purification kit (Invitrogen). RNA fragmentation, m1A-IP and library preparation were carried out according to previously published protocols(Dominissini, D. et al. The dynamic N(1)-methyladenosine methylome in eukaryotic messenger RNA. Nature 530, 441-446 (2016).)