GSM2772581: Abca3 cko AT2 cells rep2; Mus musculus; RNA-Seq
AT2 cells were purified by cell depletion as described by Corti et al. "Isolation and primary culture of murine alveolar type II cells." Total RNA was isolated from adult AT2 cells with the RNeasy Plus Mini Kit (Qiagen, Valencia, CA). RNA amplification was performed utilizing the Ovation RNA-Seq System v2 (NuGEN). RNA libraries were prepared using Nextera XT DNA Sample Preparation kits (Illumina Technologies).