GSM3259785: Norm  Input seq; Rattus norvegicus; ChIP-Seq
Genomic DNA was isolated using phenol–chloroform, precipitated with ethanol. YY1-interacting DNA was isolated with ChIP grade YY1 antibody (ab38422)  . Chromatin Immunoprecipitation was performed according to Wamstad et al. (2012) (See Supplementary Method below for detail). YY1-interacting DNA was isolated with ChIP grade YY1 antibody (ab38422). The yield of ChIPed DNA was determined via Quant IT fluorescence assay (Life Technologies) and enrichment efficiencies of ChIP reactions were evaluated by qPCR. Illumina sequencing libraries were generated with NEBNext® Ultra™ DNA Library Prep Kit (New England Biolabs) by following the manufacturer's manual. The library quality was determined by using Agilent 2100 Bioanalyzer (Agilent), and then, subjected to high-throughput 150 base paired-end sequencing on Illumina Hiseq sequencer according to the manufacturer's recommended protocol.