GSM4009037: colchicine - degradome; Hydra vulgaris; RNA-Seq
Trizol (Invitrogen) NEXTflex Small RNA-Seq Kit v3 (PerkinElmer Cat# NOVA-5132-05). Starting with 2 g of total RNA, poly(A) enrichment was performed to capture mRNAs, which was followed by 5' adapter ligation. Ligation of the 5' adaptor was done without first removing the 5' m7G cap so that only cleaved mRNAs were available for ligation. The RNA was then chemically fragmented and 3' adapters were ligated. For ligations, 25% of the standard adapter mass was used.