GSM2501449: mock_week_6; Homo sapiens; RNA-Seq
Cell samples were stored in RNALater at the time of collection. Once all samples, from week 0 to week 10, were collected, total RNA extraction was performed using Illustra RNAspin mini isolation kit (GE Healthcare) and further processed for mRNA-Seq library preparation (TruSeq RNA sample prep kit, Illumina – that starts with capture of polyA-containing transcripts), followed by cluster generation (TruSeq single-end cluster generation kit, Illumina) and high-throughput sequencing on Illumina HiSeq2000 at the Genomics Technology Facility, University of Lausanne. Addition of RNA spike-ins were also added according to manufacturer's instructions (ERCC Exfold RNA Spike-in Mixes, Life Technologies and Loven et al.) total RNA extraction was performed using Illustra RNAspin mini isolation kit (GE Healthcare) and further processed for mRNA-Seq library preparation (TruSeq RNA sample prep kit, Illumina – that starts with capture of polyA-containing transcripts), followed by cluster generation (TruSeq single-end cluster generation kit, Illumina) and high-throughput sequencing on Illumina HiSeq2000 at the Genomics Technology Facility, University of Lausanne. Addition of RNA spike-ins were also added according to manufacturer's instructions (ERCC Exfold RNA Spike-in Mixes, Life Technologies and Loven et al.)