GSM2498029: PDGF-driven glioblastoma mouse model single cell 78; Mus musculus; RNA-Seq
Cells were lysed and mRNA were first reverse transcribed into cDNA, and then pre-amplified full-length cDNA by PCR automatically using the Fluidigm C1 Autoprep instrument according to the manufacturer's instructions. Individual cDNA libraries were harvested from the microfluidic device and converted into indexed, Illumina sequencing libraries by in vitro transposition and PCR using the Nextera system (Illumina). The pooled libraries were sequenced on a single lane of an Illumina HiSeq 2000 with single-end 100-bp reads.