GSM4237389: 5i8d1: BFRTV8d miRNAseq; Capra hircus; miRNA-Seq
Extract total RNA from each sample,then total RNA was separated by 15% agarose gels to extract the small RNA (18-30 nt). After ethanol precipitation and small RNA sample enrichment by centrifugation, the libraries were prepared according to the method and process of the Small RNA Sample Preparation Kit (Illumina, RS-200-0048).The main contents are as follows: First,connecting the 3' adaptor and 5' adaptor to the separated small RNA.Then RT-PCR.Finally,recycling strips of 145-160bp (22-30nt RNA).