GSM4237503: data1_H820_E14; Homo sapiens; RNA-Seq
Frozen cell pellets were thawed and genomic DNA was extracted with a Blood & Cell Culture Midi kit (Qiagen). The amplification of sgRNA target sequences for deep sequencing was performed as described (http://genome-engineering.org/gecko) with minor modifications. All PCR products obtained from each sample were run on an agarose gel, gel-extracted, and purified according to manufacturer's recommendations. sgRNA (RNA-seq)