GSM4083986: Runx1cPos_0Dox_Rep3 RNA-Seq; Homo sapiens; RNA-Seq
Total RNA was extracted using the Bioline Isolate II RNA Mini Kit according to the manufacturers' instructions. cDNA was reverse-transcribed using random hexamer priming and Tetro cDNA synthesis kit (Bioline) or using Oligo (dT)18 priming and SuperScript™ II Reverse Transcriptase (Thermo Fisher Scientific) according to the manufacturers' instructions. RNA-seq libraries were prepared using a TruSeq Stranded mRNA Library Prep (Illumina cat# 20020594) following the Low Sample (LS) workflow according to manufacturer's instructions.