GSM4008856: CJ3_22_10_H_co_rep_1; Mus musculus; RNA-Seq
Isolated tumour cells were pelleted and lysed in QIAgen RLT buffer. RNA was extracted using a Qiagen RNeasy plus kit. Indexed PolyA libraries were prepared using 200ng of total RNA and 14 cycles of amplification with the Agilent SureSelect Strand Specific RNA Library Prep Kit for Illumina Sequencing. Paired-end 76 base-pair sequencing was carried out by clustering 2pM of the pooled libraries on a NextSeq 500 sequencer