Whole genome re-sequencing of wild barley genotypes from Tabigha microsite
Genomic DNA was extracted from third fully expended leaves of plant using QIAamp Plant DNA Mini Kit (Qiagen, Germantown, MD, USA). DNA concentration and quality was analyzed using Qubit dsDNA HS Assay Kit (Life Technologies, Carlsbad, CA, USA) and agarose gels. DNA library was constructed using Illumina Nextera DNA Library Prep Kit (Illumina, San Diego, CA, USA). DNA paired-end libraries were subjected to 2×150 bp sequencing using Illumina Hiseq X Ten sequencing platform (Illumina, San Diego, CA, USA). Clean reads of each sample were aligned against the reference genome of barley, cv. Zangqing320 (Dai et al., 2017) using BWA v0.7.5a-r405. Uniquely mapped reads were used for the SNVs and InDels calling with the Genome Analysis ToolKit (GATK) v3.6 (McKenna et al., 2010).