GSM2345452: CNOT7KD high temp 2; Mus musculus; RNA-Seq
Total RNA was extracted using Trizol, RNA was then incubated with poly(U) agarose to isolate polyadenylated mRNA. Total polyadenylated mRNA was extracted using 75˚C extraction buffer to remove all polyadenylated mRNAs from the agarose. To isolate only mRNAs with long poly(A) tails, agarose was washed with 50˚C wash buffer to remove all mRNAs with short poly(A) tails (<50) long poly(A) tails were then extracted with 75˚C extraction buffer (high-temp samples). Libraries were prepared using the Nextflex qRNA-Seq Kit v2