rs4988235 sequecnces of Medieval Homo Sapiens individual
52 bp fragment comprising LCT-13910 was amplified using LCTF (GAGAGTTCCTTTGAGGCCAG) and LCTR (GCGCTGGCAATACAGATAAG) primers fused with barcodes and Ion Torrent adapters. For each DNA extract,  2-3 separate PCR reactions were set up using AmpliTaq Gold 360 Master Mix (Life Technologies), 0.2  M each primer, and 3  l of DNA template. PCR conditions were as follows: 2 min at 94oC, 40 cycles of 1 min at 94oC, 30s at 60oC, 1 min at 72oC and final extension at 72oC for 5 min.Pooled PCR products were separated by 2% agarose gel electrophoresis and purified with QIAquick  Gel Extraction Kit (Qiagen). Fragment length distribution and concentration of libraries were measured with the use of 2200 TapeStation system (Agilent). Sequencing was conducted on high-throughput PGM Ion Torrent system using Ion 314  Chip Kit v2 and the Ion PGM Hi-Q sequencing kit v2 following the manufacturer s instructions.
Sanok034_rs4988235