normoxia1
The cDNA was sonicated using the Covaris S2 ultrasonicator, and libraries were prepared with the KAPA High Throughput Library Preparation Kit.  Samples were end repaired, 3' ends adenylated and barcoded with multiplex adapters.  PCR amplified libraries were purified with AmpureXP beads,  and validated  on the Agilent 2100 Bioanalyzer.  Before being normalized and pooled, samples were quantified by Qubit (Invitrogen) and then run on a Illumina Hiseq 2500 using SBS v3 reagents to generate 75 bp single-end reads.  ?
normoxia1