GSM1544094: LNCaP EZH2; Homo sapiens; RIP-Seq
Whole cell lysates were incubated with EZH2 antibody and EZH2-RNA complexes were isolated with antibody. Double-stranded cDNAs were generated from the reverse transcription of RNAs immunoprecipitated by EZH2 antibody and then fragmented by sonication. DNA libraries were prepared for sequencing using standard Illumina protocols.