GSM1908184: Control_small RNA-Seq #2; Homo sapiens; miRNA-Seq
For total RNA purification, Huh7.5.1 cells were lysed using TRI-reagent (MRC) and RNA purified in combination with the Direct-zol RNA MiniPrep (Zymo Research). small RNA libraries were prepared using Illumina’s protocol (Truseq small RNA, # 15004197 Rev. F). 1.2ug of total RNA was used to construct small RNA libraries.