Metagenomics study of endophytic bacteria from the root of Aloe vera plant.
Bacteria genomic DNA was extracted from the supernatants using GF-1 bacterial DNA extraction kit by Vivantis, sample normalized to concentration ≤ 10 ng/μL.PCR amplification was carried out to amplify V3-V4 conserved regions of 16S rRNA gene sequences. The PCR library preparation was carried out using KAPA HiFi HotStart ReadyMix PCR Kit (KAPA BIOSYSTEMS® U.S.A) and Nextera® XT index kit to add multiplexing indices (dual-index barcodes).And loaded onto MiSeq reagent cartridge (MiSeq Kit V2 300 cycles) for sequencing on the MiSeq system.
22-ALR