GSM2235686: VCaP_AR_R1881_30min_rep1; Homo sapiens; ChIP-Seq
Lysates were derived from sonicated (Bioruptor, Diagenode) nuclei and AR-, CTCF- and H3K9me3-DNA complexes were isolated with 1 microgram of antibody and protein-A magnetic beads. DNA fragments were purified and libraries were prepared for sequencing using standard Illumina protocol. Samples were deep-sequenced with HiSeq2000 at the GeneCore in EMBL (Heidelberg, Germany).