GSM3178817: siCTL-1; Homo sapiens; RNA-Seq
Total RNA extraction was performed using TRI-Reagent (Sigma-Aldrich) and QIAGEN RNAeasy kit. Isolation of RNA from cultured cells or human samples was performed as described previously (15,16). The RNA concentration was measured by NanoDrop 2000 (Thermo Fisher Scientific), the purity (A260/A280) of RNA was around 2.0 and the yield was in a range of 200–500 ng/ l. Then, mRNA were analyzed on a Bioanalyzer (Agilent) and only samples with RIN > 9 were used for further experiments. Paired-end 2x75b reads