GSM4082987: SN11; Mus musculus; RNA-Seq
Total RNA was extracted from biceps muscles using the ReliaPrep RNA Tissue Miniprep System (Promega), RNA quality and yield determined using Agilent BioAnalyzer and submitted for RNA-Seq analysis. RNA was amplified using the Ovation RNA-Seq system V2 (NuGEN), constructed into cDNA libraries using the Nextera XT DNA sample preparation kit (Illumina) and sequenced on the HiSeq 2500 system (Illumina, Paired-End 75bp Flow Cell) to a depth of at least 35-40 million reads