GSM1754857: Control_rep2; Mus musculus; RNA-Seq
Interscapular brown adipose tissues were isolated, and snap frozen in liquid nitrogen. Total RNA was harvested using Isol-RNA lysis reagent (5 PRIME) and the integrity of the RNA was confirmed using a Bioanalyzer 2100 (Agilent Technologies). 4 ug of total RNA from iBAT was polyA-selected, and subsequently subjected to fragmentation and cDNA-synthesis using the TruSeq RNA Sample Prep kit v2 (Illumina). cDNA-seq libraries were constructed according to the manufacturer's instructions (Illumina) as described in (Nielsen R, Mandrup S, Methods in Enzymology 2014, Vol. 537, pp. 261-279).