GSM2345441: 16-cell, unfractionated; Danio rerio; RNA-Seq
rRNA-depletion was performed using RiboZero Magnetic Gold Kit (Epicentre), unfractionated, polysome-associated and non associated RNA were subsequently isolated using Qiagen RNAeasy kit (Qiagen, USA). Sequencing library was constructed using Stranded RNA-seq libraries were generated using ScriptSeq v2 RNA Library Preparation Kit (Illumina, USA).