Illumina HiSeq 2000 paired end sequencing; RNA-seq of mouse splenocytes following infection with Listeria monocytogenes
RNA-seq of mouse splenocytes following infection with Listeria monocytogenes
Sample24h
C57BL/6 mice (Jackson Laboratory, Bar Harbor, ME) were maintained in specific pathogen-free conditions and used in accordance with the Institutional Animal Care and Use Committee. Listeria monocytogenes (clinical isolate 10403s) was from V. Boyartchuk (NTNU, Trondheim, Norway) and infected as described in PMID  24477914 for 24 hours before harvesting the spleen. Single cell suspensions from spleens were used to make total RNA. 4 μg of total RNA was used to generate libraries for RNA-sequencing (Illumina unstranded mRNA kits). Samples were sized, quantified and validated on a Bioanalyzer.