"Methane enriched sediment, high O2, replicate 1, week 4"
"16S rRNA gene universal primers 27F and 519r were utilized in a single-step PCR using HotStarTaq Plus Master Mix Kit (Qiagen, Valencia, CA). The reaction conditions were: 3 min at 94 C, followed by 28 cycles of 94 C for 30 sec, 53 C for 40 sec, and 72 C for 60 sec, followed by the final step of 5 min at 72 C."
IO2