GSM2235549: PeakMicroglia_RNAseq; Mus musculus; RNA-Seq
Illumina TruSeq Stranded Total RNA with Ribo-zero Gold Brains and spinal cords were removed and homogenized. Mononuclear cells were separated with a 30% / 70% Percoll (GE Healthcare, NJ, USA) gradient as previously reported (Pino and Cardona, 2011). Single cell suspensions from CNS were stained with anti-F4/80-APC (BM8; eBioscience, CA, USA) and anti-CD45-PerCP (30-F11; BioLegend). Cells were sorted on a BD FACSAria II (BD Biosciences). Data were analyzed with FlowJo software (TreesStar, OR, USA).  Genomic DNA and RNA were extracted from the same samples using the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen).