ChIP-seq H3K27, human maternal chr15 with a targeted deletion in the mouse F12 cell line
Four somatic cell hybrid cell lines, F12(15P), F12(15PΔIC)-1, F12(15M), and F12(15MΔIC)-1, were employed for the ChIP-seq analysis. The antibodies used were anti-H3K4me3, anti-H3K9me3, and anti-H3K27me3. Hence, in addition to input DNA, we analyzed 16 samples, S01 to S16, in total. Their libraries were prepared using the NEBNext ChIP-Seq Library Prep Master Mix Set and Multiplex Oligos. After the concentration of each library was determined using qPCR, the libraries were sequenced using the HiSeq 1500 sequencing system to generate 50-bp single-end read data.
S15