Illumina HiSeq 2500 sequencing; RNA and miRNA sequencing of extracellular vesicles derived from murine Th1 and Th2 differentiated cells
RNA and miRNA sequencing of extracellular vesicles derived from murine Th1 and Th2 differentiated cells
EV2_miRNA_exp2_s
& days differentiated Th1 and Th2 cell were re-stimulated over night with anti CD3 coated flasks. The cells were removed with centrifugation and extracellular vesicles were isolated using differential centrifugation.  RNA was isolated and purified using Norgen miRNA isolation kit. mirna library was constructed using diagenode small rna kit