GSM4007297: 2018-424/5; Mus musculus; RNA-Seq
Total RNA was extracted and treated BioRobot® EZ1 and RNA Tissue Mini Kit (Qiagen, Hilden, Germany). RNA quality and integrity were validated using a NanoDrop ND-1000 UV–vis Spectrophotometer (NanoDrop Technologies, Wilmington, USA) and Agilent 2100 Bioanalyzer and RNA 6000 Nano LabChip kit (Agilent Technologies, Palo Alto, USA) respectively. DNA libraries were prepared as using 90 ng total RNA input to the TruSeq Stranded mRNA Library Prep Kit (Illumina) according to the manufacturers instrucitons. For multiplexing, standard Illumina adaptors were used. One library was made for each individual RNA sample. The libraries were sequenced using the NextSeq Illumina platform according to manufacturer instructions, generating single end 75bp read libraries with an average library size of 25 ± 6 million reads.