GSM1545271: 1144N0003; Mus musculus; RNA-Seq
We compared RNA from dorsal and ventral MEC of 4 groups of mice. To minimize the effects of inter-animal variability and variability in the dissection, whilst maintaining sufficient power to detect dorsoventral differences, samples were pooled with 3 or 4 mice in each group. RNA was extracted using RNeasy Lipid Tissue Mini Kit (Qiagen Cat:74804).  RNA integrity was assessed using a Agilent 2100 Bioanalyzer. All sample RINs were between 7.1 and 8.5. cDNA was synthesized and amplified using the Ovation RNA-Seq System V2 (NuGEN Cat:7102) using 120 ng of starting material for each sample. The samples were fragmented and sequenced by the Ark-Genomics facility using Illumina HiSeq with multiplexed paired-end analysis on two lanes.