454-sequencing data of metatranscriptome from kimchi microbiome (J7_sample)
Total genomic DNA from six kimchi pellets (J1, J7, J13, J18, J25, and J29) was extracted using a FastDNA SPIN Kit (MPbio) according to the manufacturer's instructions. Hypervariable regions (V1 to V3) of the bacterial 16S rRNA genes from the genomic DNA were PCR-amplified using barcoded primers. The PCR products were purified using a PCR purification kit (Solgent, Korea) and their concentrations were carefully assessed in an ELISA reader equipped with a Take3 multivolume plate. A composite sample was prepared by pooling equal amounts of PCR amplicons from each sample, and pyrosequencing was performed by Macrogen (Korea) using a 454 GS-FLX titanium system (Roche, Germany). The kimchi samples were designated as Jx, where “x” indicates the sampling day during kimchi fermentation.
16S rRNA gene data sample J1