GSM2344907: miRNA.CFPAC1; Homo sapiens; miRNA-Seq
Total RNA was extracted using Trizol reagent. Illumina TruSeq Small RNA Sample Prep Kit was used with 1 ug of total RNA to produce microRNA sequencing libraries. RNA adapters specifically modified to target microRNAs and other small RNAs that have 3’-hydroxyl and 5’-phosphate group resulting from enzymatic cleavage by RNA processing enzymes are ligated to each end of the RNA molecules. After synthesis of the first strand cDNA using reverse transcriptase and random primers, the cDNA is PCR amplified with indexed Illumina oligos. cDNA libraries generated by mature microRNA are separated by size on acrylamide gels and gel purified before proceeding to HiSeq 2000 Illumina sequencing.