NextSeq 500 paired end sequencing; ChIP-seq against BZLF1 in Raji cells (EBV, Human Herpesvirus 4, GenBank accession KF717093).
ChIP-seq against BZLF1 in Raji cells (EBV, Human Herpesvirus 4, GenBank accession KF717093).
Replicate 2 Raji iBZLF1 full-length input low_p
1x10^8 Raji iBZLF1 cells were spun down (400 g, 7 min, RT) and washed with PBS. 1 x 10^8 cells were adjusted to a concentration of 5 x 10^5 cells/ ml before chromatin preparation.  Detailed protocols can be found in the most recent version of the publicly available manuscript (https://www.biorxiv.org/content/10.1101/317354v1). The library construction was performed by the company Vertis Biotechnology AG (Freising, Germany) using TrueSeq sequencing adaptors and DNA fragments of 350 to 600 nt in length.