Illumina HiSeq 2000 paired end sequencing; Establishment of Porcine and Human Expanded Potential Stem Cells (bulk RNA-seq)
Establishment of Porcine and Human Expanded Potential Stem Cells (bulk RNA-seq)
hEPSC_TSC_3_rep2_p
Total RNA was purified by RNeasy Minikit (Qiagen) according to the manufacturer's manual. RNA concentration was determined by Nanodrop, and 500 ng of total RNA was used for library construction using KAPA Stranded mRNA-Seq Kits. Sequencing was performed on Illumina HiSeq 2000 machines with 75 bp pair-end mode. RNeasy Minikit (Qiagen)  KAPA Stranded mRNA-Seq Kits