GSM2845461: RNA Library MES Rep 2; Homo sapiens; RNA-Seq
Samples for RNA were collected in RLT and lysed with a Qiashredder (Qiagen) and then RNA was purified with a Qiagen RNeasy kit. For RNA from whole hearts, samples were extracted with Qiagen RNeasy Fibrous Tissue Kit. RNA-seq libraries were prepared from total RNA using the TruSeq Stranded Total RNA Ribo-Zero H/M/R kit (Illumina RS-122-2201). Samples were barcoded together for sequencing. RNA-seq libraries were sequenced on the NEXTSEQ.