Genotype-by-sequencing of F2 interspecific hybrid between L. pruna and  L. kohalensis
We extracted DNA from whole adult crickets using the DNeasy Blood & Tissue Kits (Qiagen, Valencia, CA, USA). Genotype-by-Sequencing library preparation and sequencing were done in 2014 at the Genomic Diversity Facility at Cornell University following Elshire et al. (2011). The Pst I restriction enzyme was used for sequence digestion and DNA was sequenced on the Illumina HiSeq 2000 platform (Illumina Inc., USA) with 100 bp single end reads
PruKoh_GBS_align