Illumina HiSeq 2000 paired end sequencing; YH-fosmid_pool_2138_insertsize_316
We pooled ~30 fosmid clones and sequenced using Hiseq 2000 with different insertion sizes. We estimated that, on average, each pool contained only 0.02% of the diploid genome, therefore the DNA in each pool represented small portions of different regions on the genome and the probability that any two fosmids came from the same/overlapping genomic region was near zero.
YH-fosmid_pool_2138_insertsize_316_lib