GSM1906934: WT_in_vivo_Bisulfite; Vibrio cholerae; Bisulfite-Seq
Genomic DNA was extracted from two biological replicates of exponential phase, overnight stationary phase and frozen rabbit cecal fluid V. cholerae using the Wizard Genomic DNA purification kit (Promega). Bisulfite conversion of the DNA was carried using the EZ DNA Methylation Kit (Zymo) twice to ensure high conversion efficiency. The converted DNA was then amplified by PCR using the Kapa HiFi Uracil+ polymerase for 12-15 cycles (Kapa biosystems) and sequenced on the MiSeq platform (Illumina).