GSM4634585: NC25_NS; Homo sapiens; RNA-Seq
RNA was extracted from PBMCs using the RNeasy Mini Kit (Qiagen) and RNA integrity was assessed on an Agilent 2100 Bioanalyzer (Agilent, Palo Alto, CA). Sequencing libraries were constructed from total RNA using TruSeq RNA Sample Preparation Kits v2 (Illumina, San Diego, CA). Libraries were clustered onto a flowcell using a cBOT amplification system with a HiSeq SR v3 Cluster Kit (Illumina). Single-read sequencing was carried out on an Illumina HiScanSQ sequencer using a HiSeq SBS v3 Kit to generate 100-base single-end reads with a target of approximately 10 million reads per sample.