Rabbit ear dermal wound 6 hours post infection biological repeat A raw RNAseq reads
Total RNA was submitted to Otogenetics Corporation (Norcross, GA USA) for RNA-Sequencing. Briefly, the integrity and purity of total RNA were assessed by OD260/280 using the Agilent Bioanalyzer. Total RNA (5 ?g) was depleted of rRNA using the RiboZero Magnetic Gold Kit Epidemiology kit (Epicentre). The resulting RNA was converted to cDNA using the TruSeq Stranded Total RNA Sample Preparation kit (Illumina). The resulting cDNA was purified, fragmented by sonication (BioRuptor, Diagenode, Inc.), and profiled using the Agilent Bioanalyzer. Illumina libraries were made from the qualified, fragmented cDNA using the SPRIworks HT Reagent Kit (Beckman Coulter, Inc.) on the Biomek FXp. The quality, quantity, and size distribution of the Illumina libraries were determined using an Agilent Bioanalyzer 2100 or Tapestation. The libraries were then submitted for Illumina HiSeq2500 sequencing. Paired-end 106 or 126 nucleotide reads were generated and checked for data quality using FASTQC (Babraham Institute, Cambridge, UK).
6HI-A