GSM2165997: Nosema/BQCV2; Apis mellifera; RNA-Seq
Bees were flash frozen in liquid nitrogen and brain dissected in dry ice. RNA was extracted from pool of 4 brains using Trizol reagent and the Qiagen RNeasy Mini Kit (QIagen).  Illumina TruSeq Stranded mRNA Sample Preparation kit was used with 1 ug of total RNA for the construction of sequencing libraries. RNA-Seq libraries were constructed with the Truseq stranded mRNA sample preparation (Low throughput protocol) kit from Illumina.