DNA-Seq of multidrug-resistant Klebsiella pneumoniae strains isolated from hospitalized patients in Brazil
DNeasy 96 Blood & Tissue Kit (QIAGEN Silicon Valley, Redwood City, USA) was used to extract Genomic DNA. DNA quality was estimated with a Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, USA). Qubit (Thermo Fisher Scientific Inc., Waltham, USA) was used for DNA quantification. Libraries with an average fragment size of 550 bp were prepared for sequencing using a Illumina TruSeq DNA PCR-free LT Kit (Illumina Inc., San Diego, USA)
KpA2