Cas9 editing of AAVS1 off-target sites
SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage
1738_H7_hg38_chr6:33770090-33770112_HEK293_edited_rep_1