GSM1545838: cow_oocyte_1; Bos taurus; Bisulfite-Seq
MII oocytes were produced by in vitro maturation of GV oocytes collected from slaughter house derived ovaries according to standard protocols.  Oocyte maturation was confirmed by presence of a polar body. Then, zona pellucida and first polar body were removed by incubation in 0.5% pronase solution for 2 minutes and vigorous pipetting. Zona-free oocytes were snap frozen in liquid nitrogen and stored at -80°C until DNA extraction.  DNA from 100 cells was purified using Zymo's Quick-gDNA MicroPrep kit and the MethylC-seq libraries were prepared using Zymo's Pico Methyl-seq Library Prep Kit according to the manufacturer's instructions.