Aspergillus tamarii transcriptome on sugarcane bagasse
Following 36h incubation on Liquid Glucose, total RNA was extracted from each culture according to a standard phenol / chloroform method (Brasileiro & Carneiro, 2015). Mycelia from liquid media was collected by filtration with Whatman filter paper n 1, whilst from semi-solid cultures mycelia from the surface of each agar plate was collected manually using  a sterilized spatula. Extracted total RNA was quantified and integrity determined using an Agilent 2100 Bioanalyzer and RNA LabChip kit system (Agilent Technologies, Santa Clara, CA, USA). Isolation of mRNA, cDNA library construction and Illumina RNA-seq were conducted by Eurofins MWG Operon (Louisville, KY, USA). All treatments from the replicate bioassays were paired-end sequenced (2 x 100 bases) using TruSeq RNA Chemistry v3 on two flow cell channels of an Illumina Hiseq2000 system (Illumina Inc, San Diego CA, USA).
LG36rep2