GSM1544255: sdr1-1; Arabidopsis thaliana; Bisulfite-Seq
Genomic DNA was isolated with DNeasy Plant Mini Kit (QIAGEN) from mature leaves Briefly, sonicated DNA was end repaired with NEBnext End Repair module (New England Biolabs), adenylated, and ligated to TruSeq barcoded adaptors (illumina) with T4 DNA ligase. Following purification, samples were bisulfite converted with MethylCode Kit (Invitrogen) and then PCR amplified using Pfu Cx Turbo (Agilent) using the following PCR conditions (2 min at 95°C, 4 cycles of 15sec at 98°C, 30sec at 60°C, 4 min at 72°C and 10min at 72°C).