GSM1811352: kp_RNA-seq; Drosophila melanogaster; RNA-Seq
RNA was extracted from groups of five adult flies (1-3 days old) using TRIZOL (Invitrogen) and cleaned up using DNA-free (Ambion) cDNA libraries were prepared using an Illumina TruSeq RNA sample prep kit using three micrograms of total RNA. Briefly, poly-A containing mRNAs were using poly-T primers, RNA fragmented, first and second strand cDNA syntheses, end repair, adaptor ligation, and PCR amplification performed. The average size of the libraries cDNAs was 150 bp (excluding the adapters). Prior to sequencing the integrity and quality of cDNA libraries were assessed using an Agilent 2100 Bioanalyzer and an ABI StepOnePlus real-time PCR system