GSM2587606: SAB-24 (RNA-seq); Homo sapiens; RNA-Seq
Total RNA was extracted with Trizol and quantified using Nanodrop. RNA quality was checked using bioanalyzer (Agilent). Total RNAs were depleted from Ribosomal RNAs using RiboMinus™ technology. Libraries were prepared according to the Illumina standard protocol using TruSeq Stranded RNA Kits. Single stranded sequencing of 50bp reads was performed using Hiseq2500 (Illumina)