Illumina HiSeq 2000 paired end sequencing; RNA-seq of mouse splenocytes following infection with Listeria monocytogenes
RNA-seq of mouse splenocytes following infection with Listeria monocytogenes
SamplePBS
C57BL/6 mice (Jackson Laboratory, Bar Harbor, ME) were maintained in specific pathogen-free conditions and used in accordance with the Institutional Animal Care and Use Committee.  PBS was used as a control for infections as described in PMID 24477914 Single cell suspensions from spleens were used to make total RNA. 4 μg of total RNA was used to generate libraries for RNA-sequencing (Illumina unstranded mRNA kits). Samples were sized, quantified and validated on a Bioanalyzer.