GSM1974098: v65_2i_Rep2_5C_library; Mus musculus; OTHER
3C templates were generated   using HindIII as previously described (Dekker et al., 2002; van Berkum and Dekker, 2009). 5C libraries were generated from 3C templates using an alternating primer design across 1-2 Mb regions around Oct4, Nanog, Sox2, Nestin, Olig1-Olig2, Klf4, and a gene desert negative control  (described previously Dostie and Dekker, 2007; Dostie et al., 2006; van Berkum and Dekker, 2009).