Aedes aegypti salivary gland transcriptome from male and female adult organisms
The salivary gland library was constructed using the TruSeq RNA sample prep kit, v. 2 (Illumina Inc., San Diego, CA) following the manufacturer recommendations. The resulting cDNA was fragmented using a Covaris E210 system (Covaris, Woburn, MA). Library amplification was performed using eight cycles to minimize the risk of over-amplification. Sequencing was performed on a HiSeq 2000 (Illumina) with v. 3 flow cells and sequencing reagents. The two prepared Illumina cDNA libraries were pooled to have three times as more female than male molar amounts and the resulted, final pooled cDNA library was run on a single lane of Illumina. The read length obtained was of 101 nucleotides (nt).