GSM3104045: KI436_mRNA_HG16; Homo sapiens; RNA-Seq
For non-FUCCI cells, total RNAs were purified by Trizol (Invitrogen 15596026). After DNase treatment, mRNAs were isolated using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs E7490) and fragmented by Fragmentation Buffer (Ambion AM8740). cDNAs were synthesized using SuperScript II (Invitrogen 18064014) and used to prepare non-directional high-throughput sequencing libraries using NEBNext Ultra DNA Library Prep Kit (New England Biolabs, E7370). For non-FUCCI cells, total RNAs were purified by Trizol LS (Invitrogen 10296028). After DNase treatment, mRNAs were isolated using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs E7490). Fragmentation, cDNA synthesis, and directional library construction were performed using NEBNext Ultra II Directional RNA Library Prep kit (New England Biolabs E7760). For non-FUCCI cells, non-directional high-throughput sequencing libraries were prepared using NEBNext Ultra DNA Library Prep Kit (New England Biolabs, E7370). For FUCCI cells, directional libraries were prepared using NEBNext Ultra II Directional RNA Library Prep kit (New England Biolabs E7760).