GSM3178847: C (control, non stretched sample) rep 3; Rattus norvegicus; RNA-Seq
Cell pellets were killed in liquid nitrogen and RNA was extracted according to micro RNeasy Kit (Qiagen). Homogenization step was performed with QIAshredder columns (Qiagen). Quality check (QC) was performed (RIN=10). RNAseq was performed with the platform Illumina NextSeq500. RNA library was prepared by using polyA selection library (TruSeq stranded mRNA sample prep kit was used with 1 ug of total RNA for the construction of sequencing libraries) and sequencing mode: PE 2x75bp, 33M.