GSM3178258: RNA-Seq WT 1; Mus musculus; RNA-Seq
RNA-Seq: Hippocampi were isolated from the brains of mice at 13 to 14 months. Total RNA was extracted using TRIzol reagent followed by DNase treatment to eliminate genomic DNA contamination. ChIP-Seq: Hippocampi from wild-type C57BL/6J mice were isolated for HDAC1 ChIP-Seq. RNA-Seq: cDNA library was constructed using High throughput 3' digital gene expression (HT-3'DEG) method (Soumillon et al., 2014). ChIP-Seq: Libraries were prepared from ~1-5 ng ChIP (or input) DNA as described previously (Gjoneska et al., 2015)