GSM4083814: Alveolar macrophages, WTCS_IIa5; Mus musculus; RNA-Seq
RNA extraction of FACS sorted alveolar macrophages was performed with the miRNeasy Mini Kit (Qiagen). libraries were prepared using the TruSeq mRNA library prep kit (Illumina, San Diego, CA, USA) according to the manufacturer's instructions. A total of 25 ng of RNA was used as input for polyA selection, followed by fragmentation, reverse transcription and PCR amplification