GSM1811405: RCSsham replicate 1 (R2S); Rattus norvegicus; RNA-Seq
Neural retinas were isolated from enuclated eyes, and oriented so that only the portion of the retina with hNPCs was used (approximately half of the retina). In animals with sham surgeries, a corresponding half was used. RNA was extracted with the PureLink RNA Mini Kit (Life Technologies). Libraries were constructed using Ion Total RNA-seq Kit v2 (Life Technologies) per standard protocols.