GSM4010561: ICM_mRNA_rep1; Mus musculus; RNA-Seq
8-week-old female mice were intraperitoneally injected with PMSG and hCG. Embryos were harvested in M2 medium 98 h after hCG The Smart-seq2 libraries of ICM were prepared as previously described (Picelli et al., 2014). ICM were lysed in lysis buffer containing RNase inhibitor according to the user manual. The library was quantified using Qubit and Agilent 2100 before being subjected to sequencing.