GSM1545160: PiDcl1_24; Phytophthora infestans; ncRNA-Seq
Material from P. infestans mycelium and infected potato leaves were harvested and flash frozen in liquid nitrogen. Total RNA extraction was done with the mirVana™ miRNA Isolation Kit (Ambion), according to the manufacturer’s protocol. RNA library preparation was done according to the Illumina small RNA sample preparation kit. RNA sequencing was done using the Illumina HiSeq 2500 platform.