muscle of Paramisgurnus dabryanus Transcriptome sequencing
RNA-seq transcriptome libraries were prepared following TruSeqTM RNA sample preparation Kit from Illumina (San Diego, CA), using 5µg of total RNA. Shortly, messenger RNA was isolated with polyA selection by oligo (dT) beads and and fragmented using fragmentation buffer. cDNA synthesis, end repair, A-base addition and ligation of the Illumina-indexed adaptors were performed according to Illumina’s protocol. Libraries were then size selected for cDNA target fragments of 300–500 bp on 2% Low Range Ultra Agarose followed by PCR amplified using Phusion DNA polymerase (NEB) for 15 PCR cycles. After quantified by TBS380, Paired-end libraries were sequenced with the Illumina HiSeq 2000 (2 × 100bp read length).
AB2012M-2