GSM4633613: ECCITE-seq Arrayed GDO LENTI: THP-1; Homo sapiens; OTHER
For the scRNA/CITE/ECCITE-seq experiment: a panel of antibodies (ADTs) was used to stain the cells according to the CITE/ECCITE-seq protocol. To identify cell doublets. and keep track of experimental conditions, we split cells into aliquots and stained each aliquot with a unique hashing (HTOs) antibody.  Samples were washed and recombined and loaded on a 10X genomics chip. 5prime 10X genomics was ran and the manufacturers protocols was followed for cDNA library generation. To recover HTOs, ADTs and GDOs we followed the CITE/ECCITE-seq protocol found here: https://cite-seq.com/protocol/.