Gastric microbiota and PPIs
Experimental design: one-way reads amplicon sequencing. We amplified regions V1 to V3 of the 16S rDNA gene. Multiplex Identifiers (MIDs) were used during library preparation to barcode samples.  Each sample was PCR-amplified individually, then purified and quantitated. Equimolar aliquotes of each sample were pooled together, purified twice and quantitated again. The pooled libraries were emPCR-amplified using the Lib-L kit and unidirectionally sequenced using Titanium chemistry.