GSM3703774: female_sort_invivo4; Cryptosporidium parvum; RNA-Seq
For sorted cells RNA was isolated using the RNeasy Micro Kit (Qiagen) and processed as per manufacturer's instructions. For bulk infections, RNA was isolated using the RNeasy Mini Kit (Qiagen) and processed as per manufacturer's instructions. cDNA was generated using the SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio USA Inc) for the sorted cells, and all libraries were prepared using the Nextera XT DNA Library Preparation Kit (Illumina), according to the manufacturer's instructions.