GSM2892286: G4_3_leaf_RNA-seq; Oryza sativa; RNA-Seq
Total RNA was extracted from the twelve Oryza sativa L. spp. indica cultivars TU100 (hybrid rice) leaf samples using TRIzol (Invitrogen). RNase-free DNase I (TaKaRa) was used to remove genomic DNA from the RNA samples. Those RNA samples, with no smear seen on agarose gels, a 260/280 ratio above 2.0, and a RNA integrity number greater than 8.0 were used. Twelve cDNA libraries were sequenced by Illumina Hiseq 2500 (Illumina, San Diego, CA, USA) with 125 bp paired-end reads according to the manufacturer's instructions.