GSM3178236: IRF9KO 2h IFNb rep1; Mus musculus; RNA-Seq
Cells were washed twice with cold PBS. For total RNA isolation, 7 ml Qiazol Lysis Reagent (Qiagen) were added per 15 cm dish. 1ml of the ysate was used for RNA isolation. For DNase treatment and clean up the RNase-free DNase Set (Qiagen, #79254) and RNeasy Mini Kit (Qiagen, #74104) were used. For library preparation, the NEBNext® Poly(A) mRNA Magnetic Isolation Module together with the NEBNext Ultra II RNA Library Prep Kit from NEB #E7770L was used according to the manufacturer's protocol. The RNA quality was assessed using Agilent RNA 6000 Nano Assays that were analyzed on an Agilent 2100 Bioanalyzer and all samples displayed a RIN>9.