"Genotyping-by-sequencing data from sorghum BREAD project, Flowcell_Lane = 70MUKAAXX_2"
This sample is a multiplexed barcoded genotyping-by-sequencing library with pooled samples. It was prepared using the method of Elshire et al. (DOI:10.1371/journal.pone.0019379) with ApeKI restriction enzyme, as described in Morris et al. (DOI:10.1073/pnas.1215985110). The key for mapping barcodes to samples (n = 96) is: TCTGTGA,Blank; TTCCTGGA,Blank; AACCGAGA,IS10897; ACTA,IS10971; GTATT,IS10978; GAGATA,IS11374; ACGACTAC,IS12110; TCAC,IS13827; ATATGT,IS14206; ATTAATT,IS14216; ACAGGGAA,IS14259; CAGA,IS14276; CTGTA,IS14298; ATGCCT,IS14446; GGTGT,IS14449; AGGAT,IS14478; ATCGTA,IS14535; ATTGGAT,IS14556; ACGTGGTA,IS14571; AACT,IS14735; ACCGT,IS14830; TATTTTT,IS14963; TAGCATGC,IS15428; ATTGA,IS15466; CATCGT,IS15478; CATAAGT,IS15526; CCATGGGT,IS17593; GCGT,IS18551; GTAA,IS18698; CTTGCTT,IS18800; AGTGGA,IS18813; CATCT,IS18821; CGCGGT,IS18829; CGCTGAT,IS18835; CGCGGAGA,IS18868; TGCGA,IS18874; GGTTGT,IS18876; ATGAAAC,IS18879; TAGGCCAT,IS18919; CCTAC,IS18922; CTATTA,IS19016; CGGTAGA,IS19041; CGTGTGGT,IS19053; CGAT,IS19418; CCAGCT,IS19847; AAAAGTT,IS20205; TGCAAGGA,IS20665; GAGGA,IS20681; GCCAGT,IS20697; CTACGGA,IS20700; GCTGTGGA,IS20709; CGCTT,IS20710; TTCAGA,IS20713; GAATTCA,IS20724; TGGTACGT,IS20743; GGAAC,IS20749; GGAAGA,IS20762; GCGGAAT,IS20763; GGATTGGT,IS20782; TCACC,IS20792; TAGGAA,IS20842; GAACTTC,IS21126; TCTCAGTC,IS21401; GTCAA,IS21425; GTACTT,IS21691; TAGCGGA,IS21854; GTGAGGGT,IS21991; CTAGC,IS22074; GCTCTA,IS22239; GGACCTA,IS22291; CCGGATAT,IS22325; TAATA,IS22506; GTTGAA,IS22609; TCGAAGA,IS22632; TATCGGGA,IS22909; ACAAA,IS22986; CCACAA,IS22996; GTCGATT,IS23048; CGCCTTAT,IS23053; TACAT,IS23574; TAACGA,IS23601; CTCC,IS6973; TTCTC,IS7125; GCTTA,IS7277; AACGCCT,IS7314; AGGC,IS7722; TCGTT,IS8218; TGGCTA,IS8283; TGCTGGA,IS8347; TGCA,IS8348; AGCCC,IS9168; CTTCCA,IS9586; AATATGC,IS9597; GATC,IS9713; ACCTAA,IS9883; ACGTGTT,IS9911
Flowcell_Lane = 70MUKAAXX_2