GSM4572135: 120hpf_sibling control_2; Danio rerio; ATAC-seq
10 sibling control or uhrf1 hi272 whole embryos at 5 dpf (120 hpf) zebrafish embryos were dissociated and from each embryo, 50,000 live cells were used per library preparation. ATAC-seq: Protocol as described in Buenrostro et al (2015): doi:10.1002/0471142727.mb2129s109, with the excpetion of dissociating the livers to get cell suspension was obtained using trypsin at 37 degree for 30 minutes. Strain ued: ABNYU ATAC-seq: Protocol as described in Buenrostro et al (2015): doi:10.1002/0471142727.mb2129s109, using illumina barcoded primers for sequencing. Illumina NextSeq platform, 300 cycle, 2 X150 paired-end reads