Sus scrofa in vitro and in vivo matured oocyte transcriptomes
Total RNA was extracted from three pools of 10 oocytes for both treatments with an AllPrep DNA/RNA micro isolation kit (Qiagen). cDNA was synthesized using oligo (dT′) primed reverse transcriptase with Superscript III (Invitrogen, Grand Island, NY). Second strand cDNA was synthesized using DNA polymerase I and each sample was sequenced twice using an Illumina Genome Analyzer II.
IVV-2