GSM3762890: Elmsan1KO1_E2E8_Input_DCL_rep2; Mus musculus; ChIP-Seq
ChIP-seq for non-histone proteins (Dnttip1) was carried out with 5 x 10e7 cells and for histones with 2-2.5 x 10e7 mESCs, using a modified version of Lee et al., Nature Protocols, 2006. ChIP-seq Library Preparation and Sequencing:DNA was quantified using the Quant-iT PicoGreen dsDNA Assay (Thermo Fisher Scientific, P11496). Libraries were prepared with the KAPA HyperPrep Library Kit (Roche, 07962363001) and analyzed for insert size distribution with the High Sensitivity DNA Kit (Agilent, 5067-4626) on a 2100 Bioanalyzer or the High Sensitivity D1000 ScreenTape Assay (Agilent, 5067-5584, 5067-5585, 5067-5587, 5067-5603) on a 4200 TapeStation. Libraries were quantified using the Quant-iT PicoGreen dsDNA Assay. Single end 50 cycle sequencing was performed on a HiSeq 2500, HiSeq 4000, or NovaSeq 6000 System (all from Illumina).