Mesofilic Digestion System C2 timepoint 1 (day -203)
Microbial community DNA was extracted from the anaerobic digester sample using a MoBio soil extraction kit, and then 16S amplicons were generated with the Takahashi et al. Primers Pro341F (5'-CCTACGGGNBGCASCAG-3') and Pro805R (5' -GACTACNVGGGTATCTAATCC-3'). Nextflex indexes were incorporated onto the amplicons, and sequencing was performed using a MiSeq reagent Kit v3 (600 cycle).
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