RNA-Seq of Magnetospirillum sp. ME-1: aerobic growth replicate 2
The total RNA was extracted according to the TRIzol protocol, the quality and concentration were identified by NanoDrop 2000c spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). The rRNA was removed by Ribo-Zero? Magnetic Kit (Epicentre, USA) followed by purified after total RNA treated with RNase-free DNase I. Then 100 ng rRNA-depleted RNA were reverse-transcribed into cDNA and library construction was performed using NEB Next? Ultra? Directional RNA Library Prep Kit for Illumina (NEB, USA)
A2