RNA-Seq of Gossypium hirsutum: cultivar CS-B25
The RNA-Seq libraries were prepared using Illumina TruSeq Stranded mRNA Sample Prep Kit. The polyA mRNA was purified from 2 µg of total RNAs with oligo dT magnetic beads and subsequently random fragmented. The double-stranded cDNA was generated from the fragmented mRNA via random- priming reverse transcription and second strand cDNA synthesis procedure. After adenylating 3’-end of cDNA product, the double-stranded cDNA product was ligated with selected barcoded TruSeq RNA adapter. The final library product was prepared after 15 cycles of PCR enrichment procedure from the original adapter-ligated double-stranded cDNA template. Each final library product was validated with Qubit fluorometric quantification, Bioanalyzer analysis, and qPCR quantification before pooling for Sequencing.
RL11