GSM4083854: 106_6_S10; Rattus norvegicus; RNA-Seq
Total RNA from was extracted, DNase-treated, and purified (RNeasy, Qiagen). 1µg of total RNA underwent quality control (Bioanalyzer; all RIN values > 7.0) and library construction for polyA+ RNA sequencing. Directional polyA+ RNA llibraries were constructed at the UAB Helfin Genomics Core. Libraries were quantified and underwent sequencing (~25M total 75bp paired-end reads) on an Illumina sequencing platform (NextSeq 500).