GSM3702310: mCh+Rep1; Mus musculus; RNA-Seq
Tumors from PyMT; Dll1-mCherry and PyMT; Dll1-GFP mice were digested with collagenase/hyaluronidase using method obtained from Shackleton et al, 2006. Single cells were sorted for epithelial cell enriched GFP/mCherry+  and GFP/mCherry- tumor cells. Epithelial cell enriched population was obtained by sorting out and removing stromal cells with CD45, CD31 and ter119 antibodies. RNA was harvested using Purelink RNA Mini Kit cat#12183018A with 500 ug of total RNA for the construction of sequencing libraries. Illumina TruSeq Stranded Library Prep Kit