Whole genome sequencing of a cultivated aus individual putatively ancestral to BHA weeds
Samples were prepared using an Illumina protocol similar to what is outlined in  “TruSeq DNA Sample Preparation Guide” (Ambry Genetics Part# 15005180 Rev. A, Nov. 2010).  DNA was fragmented and DNA fragment ends were repaired and phosphorylated using Klenow, T4 DNA Polymerase and T4 Polynucleotide Kinase.  Next, an ‘A’ base was added to the 3’ end of the blunted fragments, followed by ligation of Illumina Adapters via T-A mediated ligation.  The ligated products were size selected by gel purification and then PCR amplified using Illumina Paired-End primers.  The library size and concentration were determined using an Agilent Bioanalyzer.