WGS of Campylobacter jejuni strain A14a
Libraries were prepared using the KAPA Low-Throughput Library Preparation Kit with Standard PCR Amplification Module (KAPA Biosystems) according to the manufacturers instructions except with the following slight modifications: 750 ng of DNA was sheared at 30 psi for 40 s and size selected to 700-770 bp following Illumina MiSeq protocols. PCR reaction was run with 3-5 cycles, using TruSeq LT and PCR primers (standard desalted, Integrated DNA Technologies; IDT) at final concentrations of 0.375 and 0.5 mM, respectively. Libraries were quantified using the KAPA Library Quantification Kit (KAPA Biosystems) and again with digital droplet PCR (ddPCR) on a QX200 system (Bio-Rad) using the Illumina TruSeq ddPCR Library Quantification Kit both using the manufacturers instructions except the ddPCR reaction had an additional 2 min annealing/extension time.
Cj_str_A14a