GSM1973979: hNEC-nucleosome; Homo sapiens; MNase-Seq
~2*10^7 cells were cross-linked,then were digested with 2.5μl MNase(NEB# M0247S; 2,000 gel units/μl) at 37℃ for 20 min to generate a majority of mononucleosomal fragments (>=80%) with some smaller and di-nucleosome-sized fragments.Only mononucleosomal DNA fragments (~150 bp) were gel purified, pooled and prepared for sequencing. Libraries were prepared for sequencing using standard Illumina protocols.