GSM3259362: P50_WT_Dorsal_Epidermis_RNAseq; Mus musculus; RNA-Seq
Total RNA was isolated from FACS-purified cells lysed with Trizol (Life Techologies) followed by extraction using a direct-Zol RNA mini prep kit (Zymo research). For cDNA synthesis, equal amounts of RNA were added to re¬verse-transcriptase reaction mix (Vazyme, R222-01). RNA libraries were prepared for sequencing using standard Illumina protocols.