MinIon 16S rRNA gene amplicon sequencing of 6 months incubated sample 2
PCR amplification of the 16S rRNA gene using the 16S Barcoding Kit (SQK-RAB204) and the LongAmp Taq 2x Master Mix. The PCR products were cleaned using magnetic beads and pooled. The library was loaded in the SpotON port of a R9.4.1 flow cell and run using a MinION MK1B device and MinKNOW 2.0.
Min-2-6