GSM2498527: Pdgfra-cre-GFP positive cells 17; Mus musculus; RNA-Seq
Forebrain and spinal cord were excised and dissociated with the Papain Neural dissociation kit from Miltenyi, following the manufacturer’s instructions. Variable, see sample Illumina’s TruSeq Stranded Total RNA Library Prep Kit was used according to manufacturer’s instructions. Three pooled biological replicates were sequenced for each developmental stage and brain region, and a total of 38 - 66 million 150 bp strand-specific paired-end reads were generated for each replicate, comprising a total of 620.5 million reads across all datasets