GSM3863058: RNA-Seq-Col-Heat-Rep1; Arabidopsis thaliana; RNA-Seq
Total RNA was extracted from seedlings by using RNeasy Plant Mini Kit (Qiagen) with RNase-Free DNase (Qiagen) Poly-A RNA-Seq library preparation and high-throughput sequencing were performed by Beijing Novogene Co. Ltd. NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) was used to generate sequencing libraries following the manufacturer's recommendations. The libraries were further sequenced by Illumina Hiseq 4000 platform and paired-end 150bp reads were generated.