GSM3178251: R1_DRHM_EpiLC_H3K27ac; Mus musculus; ChIP-Seq
Cells were crosslinked on plates with 1% formaldehyde in media for 15 minutes at room temperature. 50 million cells were used per ChIP reaction. Whole cell extracts were sonicated on a QSonica Q700 tip sonicator to solubilize the chromatin. The chromatin extracts containing DNA fragments with an average size of 500 bp were immunoprecipitated using different antibodies. Library construction was performed by the Whitehead Institute Genome Technology Core using the Swift Biosciences Accel-NGS 2S Plus DNA Library Kit according to manufacturer's protocol.