Amplified 16S rRNA sequences from bacteria naturally present on basil leaves (Ocimum basilicum): Sample sample_B15d14
"Extraction total microbial DNA (NucliSENS easyMAG, Biomerieux); Amplification of the V1, V2 and V3 region of the 16S rRNA gene with primers 27F and 533R (5'-AGAGTTTGATCCTGGCTCAG-3' and 5'-TTACCGCGGCTGCTGGCAC-3') with barcodes for multiplexing; Purification of PCR products with PureLink PCR Purification Kit (Life Technologies); Pyrosequencing with GS FLX Titanium 454 (Roche) at Beckman Coulter Genomics USA."
"Basil V, d14 15 degrees Celsius "