RNA-seq of Mentha x piperita: salicylic acid treatment at 2 mM, biological replicate 3
Mentha piperita plants of 45 days post-germination were treated or not with salycilic acid (SA) 2 mM by foliar applications. These plants were used in the differential gene expression evaluated. Plants of one week after foliar SA applications were used to extract total RNA from young leaves using TRIzol ® reagent (Invitrogen Carlsbad, CA, USA) and purified using RNeasy kit (Qiagen). The contaminating genomic DNA was removed using Dnase I, and RNA was quantified using a Nanodrop 2000 (Thermo Fisher scientific). Three replicates of cDNA libraries were prepared, clustered and sequenced usingthe technology HiSeq 2000 (Illumina) was carried out at LANGEBIO (Irapuato,Mexico).
SA_R3