HSV1 Strain KOShysyn
"Barcoded sequencing libraries for each virus were prepared following the manufacturer's protocol for sequencing of genomic DNA (Illumina TruSeq DNA Sample Prep Kit). Viral nucleocapsid DNA (1-5 _g) was used as the input for library preparation. Five to six libraries were multiplexed per flowcell lane; the DNA fragment size selected for each library was centered at 550 bp. Libraries of HSV-1 KOS and F sub-clones were sequenced at the Sequencing Core Facility of the Lewis-Sigler Institute for Integrative Genomics (Princeton University), using paired-end, 100 bp sequencing protocols on an Illumina HiSeq2000 with version 2 chemistry. Illumina Real-Time Analysis (RTA 1.12.4.2) software provided image analysis and base calling under default settings."
HSB1 Strain KOShysyn library 1