GSM2233118: sir2Δ-KO2 rep1; Kluyveromyces lactis; RNA-Seq
Hot phenol treatment was used to harvest total RNA. Turbo Dnase (Life technologies) was used to remove DNA. Samples were concentrated using a Qaigen RNA column. RNA samples were processed for sequencing using the TruSeq non-stranded RNA Library Prep Kit (Illumina), and 50 bp single end sequencing was performed on an Illumina HiSeq 2000 machine at the Duke IGSP sequencing facility.