GSM3763194: R35-07; Homo sapiens; RNA-Seq
mRNA was isolated from sorted circulating Tfh cells (CD4+CD45RA-CXCR5+PD1+ICOS+CD38+ cells) using the SMART-SeqV4 Ultra-low input RNA kit (Takara-Clontech) and sorting 200 cells directly into lysis buffer. cDNA libraries were subsequently generated using the Nextera XT DNA kit (Illumina).