RNA-Seq of ZY458 and its gene knockout mutants
Total bacterial RNA was extracted using TRIzol® reagent (Invitrogen, Carlsbad, CA), according to the manufacturer’s instructions. The integrity and purity of RNA were assessed using agarose gel electrophoresis and spectrophotometer (Eppendorf, Hamburg, Germany). Library construction and sequencing were executed by the Beijing Institute of Genomics (BGI, Shenzhen, China).