GSM4633620: miRNA HaCaT cells-unexposed sodium arsenite maintained for 7 weeks-replicate 2; Homo sapiens; miRNA-Seq
Total RNA was purified from the cells using the mirVana™ miRNA Isolation Kit. The Truseq Stranded mRNA kit was used to prepare mRNA libraries from 1 μg total RNA. Libraries were validated on the Agilent 2100 Bioanalyzer and quantitated using the Illumina Library Quantification Kit, ABI Prism qPCR Mix from Kapa Biosystems and the ABI7900HT real-time PCR instrument. All samples were pooled and run simultaneously on 4 flow cells, using 2x150 paired end sequencing with the 500 High-output v2 (300 cycles) sequencing kit on the Illumina NextSeq500 instrument.