Amplicon-seq of weissella confusa strains
genomic DNA was extracted using DNeasy blood & tissue (QIAGEN). 16SrRNA gene was amplified with primers forward 27F: 5'-AGA GTT TGA TCM TGG CTC AG-3, reverse 1492R: 5'-CGG TTA CCT TGT TAC GAC TT-3, forward 500F: 5'-GCG TAC TAT CTA AGG CTG TTA A-3) and reverse 800R: 5'-TAC CAG GGT ATC TAA TCC-3. PCR amplification was done with the Phusion Hot Start II DNA Polymerase (2 U/L) kit (Thermo Fisher) PCR products were then purified from their agarose gel using an E.Z.N.A gel extraction kit (Omega BioTek). DNA sequencing was done by AUSTRAL-omics, a unit from the Austral University of Chile (Valdivia, Chile).
9F