GSM2773029: LSK cells from K9M mice-replicate 3; Mus musculus; RNA-Seq
Bone marrow was harvested from mice and subjected to red blood cell lysis in ACK buffer.  The sample was then filtered and stained for lineage markers (Mac1, Gr1, B220, CD3e, Ter119), Sca1, and cKit.  Cells were sorted via FACS for lineage marker negative, Sca1 positive, cKit positive cells.  Resulting cells were processed using a Qiagen RNeasy Plus Micro kit. cDNA was generated using Truseq total RNA kit (Illumina Inc.) according to the manufacturer's protocol. End repair, A-tailing, adapter ligation  and PCR amplification was carried out according to manufacturer's instructions with modifications described elsewhere (Bowman et al., BMC Genomics 2013).