GSM2344503: Dgcr8+/- NSC_1; Mus musculus; RNA-Seq
Total RNA was from NSCs the DirectZol RNA Kit (Zymo Research). RNA integrity (RIN) was verified on an Agilent 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Paired-end sequencing libraries were prepared by the Genomic Services Lab at HudsonAlpha Institute for Biotechnology (Huntsville, AL) followed by quality control, cluster generation and sequencing on the Illumina HiSeq 2000 platform. The reads were de-multiplexed and converted to FASTQ format using CASAVA software from Illumina (by the Mayo Clinic core).