GSM4634028: D2_latent_resting; Homo sapiens; miRNA-Seq
Total RNA was extracted using the RNeasy Plus Mini Kit (cat. #74134), according to manufacturer's instructions, with addition of extra ethanol to the flow through from the gDNA columns (1.5 volumes of 100% Ethanol) to recover small RNAs. On-column DNase treatment was included as part of the protocol to remove genomic DNA. Library construction and sequencing were conducted at Expression Analysis, Inc. Briefly, the Illumina TruSeq Small RNA Library Prep kit was used to prepare libraries, and the BluePippin system was used for size selection. Libraries were sequenced to a read depth of 5 million reads using the Illumina HiSeq2000 to generate 50 base pair single end reads.