MiRNA sequence from Nicotiana benthamiana flower sample
The small RNA cDNA libraries was constructed using the Small RNA Sample Prep Kit (Illumina, CA, US), according to the manufacturer’s instructions. Briefly, 1 µg of total RNA from wild-type Nicotiana benthamiana samples, including, seedling, root, leaf, stem and flower were ligated to Solexa adaptors and then converted to cDNA by RT-PCR. The resulting cDNAs were then amplified by PCR, gel-purified and submitted for Illumina/Solexa sequencing.
mirna_flower