GSM3763232: PLA35_LN_3; Homo sapiens; RNA-Seq
mRNA was isolated from sorted T helper cell populations (CD4+CD45RA- cells and markers CXCR5 and PD1 as described in the sample information using the SMART-SeqV4 Ultra-low input RNA kit (Takara-Clontech) and sorting 40-1000 cells directly into lysis buffer. cDNA libraries were subsequently generated using the Nextera XT DNA kit (Illumina).