GSM4633914: Macaque_Placenta_ChIP-Seq_H3K27ac_rep2; Macaca mulatta; ChIP-Seq
Placenta samples for Rhesus monkey were collected following previous study (Dunn-Fletcher, et al. 2018). Two placenta samples were collected by cesarean section, and frozen at -80 degree for use. ChIP-Seq for placenta samples was performed using the MAGnifyTM Chromatin Immunoprecipitation System (ThermoFisher, 492024). Chromatin fragmentation was performed using Diagenode Bioruptor Plus Sonicator. The antibodies used include H3K4me1 (Abcam, ab8895), H3K4me3 (Abcam, ab8580), H3K27ac (Abcam, ab4729). ChIP-Seq libraries were constructed using Takara SMARTer ThruPLEX DNA-Seq Kit (Takara, R400674), and then sequenced as 50 bp single-end reads with HiSeq2500 (Illumina).