GSM1812032: cFD + spt cMO_replicate 5; Danio rerio; RNA-Seq
After collection of 5 biological replicates, total RNA was obtained from each sample using the RNAqueous-Micro Total RNA Isolation Kit (Ambion) and RNA integrity was assessed by bioanalyzer analysis (RIN 8.1 - 9.9) Libraries were constructed according to the CEL-seq protocol (Hashimshony, T et al.  Cell Reports. 2012).  Barcode information on read_1 of paired-end reads: cntrl_1, CATCACGC; cntrl_2, GTCGTTCC; cntrl_3, GTCGTGAA; cntrl_4, TCACACGC; cntrl_5, TCACAGAG; cMO_1, TGATGCGC; cMO_2, ACGACTCC; cMO_3, ACGACGAA; cMO_4, ATGTGCGC; cMO_5, CGTGTGAG.