GSM2774589: Young pod; Cicer arietinum; Bisulfite-Seq
Genomic DNA was isolated using Qiagen DNeasy Minikit. DNA samples were  fragmented via sonication to generate 100-300bp fragments. The fragments  were end repaired, followed by ligation with TruSeq-methylated adapters.  500ng adapter-ligated DNA fragments were used for bisulphite conversion using EZ DNA Methylation-GoldTM kit (Zymo Research Corporation, CA, USA)  according to the manufacturer's instructions. Library quality was analyzed after desalting, size selection and PCR amplification.