Metagenomic sequencing of the upper limit of the Arabian Sea oxygene minimum zone
Genomic DNA was sheared for 7 minutes using the Ion Xpress™ Plus Fragment LibraryKit following the manufacturer’s instructions. Further library preparation was performed using the Ion Plus Fragment Library Kit following manufacturer’s instructions. Size selection of the library was performed using an E-gel 2% agarose gel, resulting in a median fragment size of approximately 330 bp. Emulsion PCR was performed using the OneTouch 200 bp kit and sequencing was performed on an Ion Torrent PGM using the Ion PGM 200 bp sequencing kit and an Ion 318 chip, resulting in 4.9 million reads for station PA2