Illumina HiSeq 2000 sequencing; Transcriptional regulation of endocrine cell differentiation by Insm1
Transcriptional regulation of endocrine cell differentiation by Insm1
E15.5 Insm1^GFP/+ library 2
Total RNA was isolated using TRIzol LS (Invitrogen) and column purification (Zymo Research), according to manufacture's instructions. RNA was DNase-treated (Ambion) and integrity was assessed on the Bioanalyzer (Agilent). Linear amplification The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008).