Evolution of Avena spp. as revealed by maternal lineages
Up to 15 g of 48 hour dark incubated 10 day old seedling leaf tissue was frozen in liquid nitrogen and ground in a mortar to a fine powder. Chloroplasts were released into an isotonic buffer, filtered through Miracloth, and washed in isotonic buffers through several selective centrifugation steps. Total cpDNA was isolated using Qiagen DNEasy Plant Mini kit and the library was prepared using Illumina's Nextera XT Library Preparation Kit.
CN22002_S4