GSM1318160: OMA1_RIPSeq; Caenorhabditis elegans; RNA-Seq
Lysates were clarified from sonicated animals. OMA-1-RNA complexes were isolated with antibody and eluted from the immunopurification matrix with AcTEV protease.  RNA was isolated with Trizol Reagent and purified with RNAqueous-Micro columns. A TruSeq RNA library was prepared with no poly-A mRNA purification, using standard Illumina protocols. 5 microliters purified RNA from an OMA-1 immunopurification was sequenced.