18S V4 sequences of Thalassiosira community
A 420-bp 18S V4 gene fragment was PCR amplified from BioSamples' genomic DNA using a pair of 18S primers modified by adding illumina adaptors to the 5' ends. PCR amplicons were processed for sequencing DNA library using Illumina Nextera kit and sequenced in a multiplexed sample on a single flow cell of Miseq platform for 250 bp paried-end reads.
SF18