Full-length RNA-Seq of bermudagrass
The library construction and PacBio sequencing was performed according to the official protocol as described by Pacific Biosciences. Briefly, 1  g total RNA extracted from each 5 tissues were equally pooled together and were used as input for cDNA synthesis using SMARTer PCR cDNA Synthesis kit. A total of 23 PCR cycles of amplification was performed using KAPA HiFi PCR Kits. Amplification was followed by size selection using the BluePippin Size Selection System with two bins: 0.5-2 and 2-6 kb. After size selection, another amplification was performed using 12 PCR cycles. The cDNA products were then subjected to construction of SMRTbell template libraries using SMRTbell Template Prep Kit. Finally, the SMRT cells were sequenced on PacBio RS II instrument using P6-C4 polymerase and chemistry with 6 h movie times.
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