GSM4634776: N-term-mCherry-Pdr802 IP rep 3; Cryptococcus neoformans; ChIP-Seq
The remaining material was incubated with rabbit IgG anti-mCherry antibody (Abcam, ab213511) tethered to protein A sepharose ('IP') or sepharose alone ('Mock') overnight at 4°C. The beads were then washed, incubated at 65°C to reverse DNA-DNA and DNA-protein crosslinks and the DNA recovered by phenol/chloroform/isoamyl alcohol (25:24:1) extraction, ethanol precipitation, and resuspension in nuclease-free water. The samples were sonicated to obtain fragments of about 200bp. Libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina E7645L, following the manufactrer's instructions