J2001 amplicon sequencing
"RNA samples were obtained from whole blood of Ebola virus (EBOV) infected patients and were reverse transcribed to cDNA. EBOV-specific primer pairs with overlaps were designed, and singlex PCR amplification was performed. PCR products of each sample were pooled and prepared multiplex NGS library by using Nextera XT Sample Preparation Kit. Illumina MiSeq platform was employed to generate 2 x 150-bp pair-ended reads."
J2001