GSM1907992: Sample_FP2; Oryza sativa Indica Group; ncRNA-Seq
Total RNA was extracted from rice young panicle at the pollen mother cell formation stages and meiosis stage using the Trizol reagent (TaKaRa, Dalian, China) according to the manufacturer’s instructions. Small RNAs ranging 18-30nt were size fractionated from 15% polyacrylamide gel electrophoresis (PAGE), isolated from the gel and ligated with the 5’ and 3’ RNA adaptors using T4 ligase. The resulting products were reverse transcribed and amplified using 15 PCR cycles to produce sequencing libraries.