Illumina HiSeq 1500 sequencing
Strand-specific RNAseq library
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Library preparation and Illumina sequencing were performed at the Ecole normale superieure Genomic Platform (Paris, France). Ribosomal RNA depletion was performed with the Ribo-Zero kit (Epicentre), using 1 microgram of total RNA. Libraries were prepared using the strand specific RNA-Seq library preparation ScriptSeq V2 kit (Epicentre). Libraries were multiplexed by 4 on flow cell lanes. A 50 bp read sequencing was performed on a HiSeq 1500 device (Illumina) . A mean of 47,5 +/- 11 millions passing Illumina quality filter reads was obtained for each of the 4 samples.