GSM2771832: ino80-1; Saccharomyces cerevisiae; RNA-Seq
Pellets were collected, snap-frozen and then processed as per the RNA-seq protocol outlined. Two biological replicates were collected(-1,-2 or -3,-4 or -5,-6). RNA was prepared from samples (approximately 1.5 ODs) using the MasterPure™ Yeast RNA Purification Kit (Epicentre, MPY03100). The sequencing libraries were prepared from 0.8 µg of RNA/sample using the Illumina TruSeq Stranded mRNA kit (Illumina, 15031047). The quality of the pooled library was checked using the Agilent Bioanalyser 2100 HS DNA assay. The library was sequenced on an Illumina HiSeq 2000 platform.