GSM1907934: TMRM Low; Mus musculus; RNA-Seq
Total RNA was used to synthesize cDNA. 220-400 bp fragments were isolated using 2% E-Gel (Invitrogen), ends were repaired, adaptor was added using T4 DNA ligase (New England Biolabs), and amplified for 17 cycles using PE 1.0 and PE 2.0 primers (Illumina) and Phusion High Fidelity PCR Master Mix (New England Biolabs). PCR products were barcoded (indexed) and sequenced on Illumina HiSeq 2000 platform