GSM1138116: Orange-F; Citrus sinensis; RNA-Seq
All samples were collected and frozen in liquid nitrogen immediately after collection and kept at -80℃ until use.Total RNA was extracted using Trizol reagent. Size fractionated small RNAs (16-30 bp) from total RNA extracts was ligated to 5' and 3' adapters, and reverse transcribed. After PCR amplification the sample was subjected to Solexa sequencing.