Genome sequence of bovine-adapted Staphylococcus aureus
Genomic DNA (2.5 ug) was sonicated in 55 uL of buffer EB (Qiagen, Germany) using a bioruptor (Diagenode) for 30 seconds on, 90 seconds off for a total sonication time of 10 minutes and a target fragment size of 550 bp. Fragments were end-repaired, size selected, A-tailed and Illumina adapters ligated according to the Illumina TruSeq DNA PCR-free sample preparation kit instructions. The libraries were sequenced using an Illumina MiSeq v2 500-cycle kit.
MOK98_S18_L001