Effect of CamSA on the hamster and mouse models of Clostridium difficile infection
Amplification and sequencing of the V4 region of 16S rRNA genes of Bacteria and Archaea was performed essentially as described in Kozich et al. (2013) with the following modifications: to be more inclusive of several archaeal lineages, forward primer 515F was modified to contain a C or T at the 4th position from the 5' end (5' GTGYCAGCMGCCGCGGTAA), and a corresponding modification was made to the read 1 sequencing primer; 5 Prime HotMasterMix DNA polymerase was used (#2200410, 5 Prime Inc., Gaithersburg, MD, USA); 33 cycles were used in PCR; sequencing on the Illumina MiSeq platform was performed at Micro-Seq Enterprises (Las Vegas, NV, USA).
MMCamE14