GSM4008314: Control_Ascl1_ChIPseq; Mus musculus; ChIP-Seq
P0 retinas or cultured, post-natal day 12, Müller glia (+/- Ascl1 overexpression, rtTA germline:tetO-Ascl1-ires-GFP mice ± doxycycline) were digested with papain/DNase to single cells and fixed with 0.75 % formaldehyde for 10 minutes at room temperature.  Sonication was performed with a probe sonicator (Fisher Scientific): 12 pulses, 100 J/pulse, Amplitude: 45, 45 seconds cooling at 4 ºC between pulses.  Immunoprecipitation performed with 40 mL anti-mouse IgG magnetic beads (Invitrogen Cat: 110.31) and 4 mg mouse anti-MASH1 antibody (BD Pharmingen Cat: 556604) against chromatin from 5 million cells per condition according to Diagenode LowCell Number Kit using IP and Wash buffers as described in28. Libraries were prepared with standard Illumina adaptors and sequenced to an approximate depth of 36 million reads each.  Sequence reads (36 bp) were mapped to the mouse mm9 genome using bwa (v 0.7.12-r1039).