GSM2691277: RNA E13.5 forebrain r2; Mus musculus; RNA-Seq
We first extracted total RNA using RNeasy Lipid tissue mini kit from Qiagen (cat no.#74804). We used Truseq Stranded mRNA LT kit (Illumina, RS-122-2101 and RS-122-2102) to constructed stranded RNA-seq libraries on 4ug of the extracted total RNA.