small RNA sequencing of Chlamydomonas reinhardtii CC503
The C. reinhardtii cells were grown in  TAP medium at 22-26Ąć under a 12:12h light/dark cycle. Four-day-old cells were cellected and total RNA was extracted with Trizol reagent (Invitrogen). 1ug RNA was used fro the standard small RNA library construction according to the manufacturers' protocols using NEBNext Small RNA Library Prep Set for SOLiDTM ( NEW ENGLAND BioLabs).
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