GSM4633881: Untreated_P260_BiolRep_4; Mus musculus; RNA-Seq
Total RNA was prepared by homogenizing liver pieces in 10 volumes of Nucleozol reagent followed by the addition of 2 volumes of chlorophorm and centrifugation at 12.000 g for 15 minutes. The aqueous phases were precipitated with isopropanol and subjected to a second extraction as above, but with Trizol reagent. The RNA samples were digested with 10 units of DNase I for 10 min at 37 0C, followed by purification with phenol/chlorophorm extraction and ethanol precipitation. RNA-seq libraries were generated using QuantSeq 3' mRNA-Seq FWD kit from Lexogen and sequenced in an Illumina NextSeq 500 system. RNA-seq, 3'Quant-seq