ChIP-seq of IP_pGNL::GNL:HA gnc gnl_Arabidopsis
Seedlings of pGNL::GNL:HA gnc gnl and gnc gnl were growing under long day conditions (16 hours light - 8 hours dark) on 22 °C for 10 days on GM agar plates. At the tenth day seedlings were collected and fixed in 1% Formaldehyde solution. It followed a standard ChIP protocol for plant material and after positive controls verification by qRT-PCR, the ChIPed-DNA for each sample sent for next generation sequencing. Antibody for the ChIP-IPs was the Anti-HA tag ChIP-grade from Abcam (ab9110). Additionally, the subsequent ChIP-seq analysis conducted by the use of the SOAPv1 (Li, Li, Kristiansen, & Wang, 2008) for the mapping and CSAR (Muiño, Kaufmann, van Ham, Angenent, & Krajewski, 2011) for the peaks detection.