DNA metabarcoding of Corynorhinus rafinesquii: guano pellet
DNA was extracted from bat guano utilizing a CTAB procedure. Following extraction the sample was subjected to a two step PCR protocol; first universal primers were used to individually amplify markers before preforming a barcoding PCR. After which samples subject to bead clean up and pooled in equimolar concentration. Sequencing was conducted on an Illumina Miseq.
Run5-86