ChIP-Seq of Ciona intestinalis, Whole Embryo, e. gastrula stage, WT, rep1, H3K4Me3
For each sample, embryos batches were snapfrozen in liquid nitrogen at the early gastrula stage and kept at -80C. After crosslinking with 16% formaldehyde for 10 minutes at room temperature, chromatin was sonicated with a Bioruptor to an average size of 200bp. Sonicated chromatin was immunoprecipitated with Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (Abcam, Ref : ab8580). ChIP-seq library was constructed with an Illumina kit was constructed. Sequencing (50SE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China).
Ciinte-WE-eGast-WT-R1-H3K4me3