16s amplicon of environmental soil: rhizosphere: Sample sample_bact23
"DNA extracts were amplified in double with 515F and 806R 16S rRNA bacterial primers comprising the V3 and V4 hypervariable regions. PCR products were pooled, purified with PureLink PCR Purification Kit and quantified with Qubit 2.0 Fluorometer. Libraries were prepared using the Nextera XT kit, purified using AMPure XP beads, normalized, pooled and sequenced using the Illumina MiSeq 2x250bp paired-end chemistry."
library_bact23