ENDIA_QC  BCM_330-3 16S
16S rDNA sequencing methods were adapted from the methods developed for the NIH-Human Microbiome Project (1,2). Briefly, bacterial genomic DNA was extracted using MO BIO PowerSoil DNA Isolation Kit (MO BIO Laboratories) following the manufacturerÕs instructions. The 16S rDNA V4 region was amplified by PCR and sequenced in the MiSeq platform (Illumina) using the 2x250 bp paired-end protocol. The primers used for amplification contain adapters for MiSeq sequencing and single-end barcodes allowing pooling and direct sequencing of PCR products (3).
BCM_330-3