DNA_seq from B.megaterium RmBm31
Glycerol stock of Bacillus megaterium RmBm31 strain was streaked on LB medium and grown 24 hours at 28C. One colony forming unit (CFU) was used to inoculate LB liquid medium Total DNA was isolated and purified using NucleoSpin Microbial DNA kit (MACHEREY-NAGEL). RNAse treatment was performed according to manufacturer instructions. DNA integrity was checked by agarose gel electrophoresis and quantification was performed on a Qubit fluorometer using a Qubit DNA HS Assay Kit (Thermo Fischer Scientific). The absence of RNA contamination was checked using a Qubit RNA Assay Kit (Thermo Fischer Scientific).
B_meg_seq