GSM3178304: scr_si_rep3; Homo sapiens; RNA-Seq
After RNA isolation using RNeasy Mini Kit (Qiagen #74104), followed by 30 min DNase treatment (Ambion AM2238) at 37°C. Poly(A)+ RNA transcript was isolated (NEBNext Poly(A) mRNA Magnetic Isolation Module - NEB #E7490) from 1 microgram of total RNA for RNA library preparation and sequencing using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB # E7420S) according to the manufacture's instruction.