GSM1543604: RNA-Seq-of-WT-ESC-RA-treated.rep1; Mus musculus; RNA-Seq
Chromatin Immunoprecipitation were performed according to Diagenode IDeal ChIP-seq Kit with minor modifications. Briefly, 2 million cells were harvested in PBS and crosslinked in 1% formaldehyde for 2 minutes. After sonication, chromatin from 1 million cells was subjected to ChIP using IDeal ChIP-seq Kit. For RNA-Seq, RNA samples were extracted using Trizol reagent, and genomic DNA contaminations were eliminated with TurboTM DNase (Life technologies). ChIP-seq libraries were constructed with KAPA Library Preparation Kits and single-end sequenced with Hiseq 2000. For RNA-Seq, total RNA were extracted and the subsequent library preparation steps were carried out according to standard Illumina procedures at the NIBS sequencing center and subjected to single-end sequencing.