GSM1642474: relAspoT 16hr Rep 2; Haemophilus ducreyi 35000HP; RNA-Seq
Bacteria was collected and RNA was harvested using Trizol reagent. The Ribo-Zero Magnetic kit was used to remove rRNA and the Illumina TruSeq Stranded mRNA sample preparation kit (Cat# RS-122-2101) was used with 10 to 100 ng of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols.