Illumina HiSeq 2000 paired end sequencing
Exome sequencing
NA
DNA randomly fragmented with peak of 150 to 200bp. Aadapters were ligated to both ends. Purified by the Agencourt AMPure SPRI beads and fragments with insert size about 200bp were excised. Amplified by LM-PCR, purified, and hybridized to the SureSelect Biotinylated RNA Library (BAITS) for enrichment. Hybridized fragments were bound to the strepavidin beads whereas non-hybridized fragments were washed out after 24h. Captured LM-PCR products were subjected to Agilent 2100 Bioanalyzer to estimate the magnitude of enrichment.