GSM3258535: Tubma-FB3; Tuber magnatum; RNA-Seq
Total RNA from T. magnatum free-living mycelium and fruiting bodies was extracted  using the Spectrum Plant Total RNA kit (Sigma-Aldrich), following the protocol A and the  on-column DNase Digestion procedure. Total RNA was extracted from ectomycorrhizal root  tips of T. magnatum/Q.robur using the RNeasy Plant Mini Kit of Qiagen with DNase step and  addition of 20 mg/ml polyethylene glycol to the RLC extraction buffer. cDNA libraries were prepared for sequencing using standard Illumina protocols by GeT (Génopole Toulouse Midi-Pyrénées, Auzeville, France)