GSM2159807: MCF-7 cell line pre-miR-29b-1 transfected replicate 1; Homo sapiens; RNA-Seq
RNA was isolated using the Exiqon miRCURYTM RNA Isolation kit and its concentration and quality assessed using a NanoDrop spectrophotometer. Quantitative real-time PCR confirmed overexpression of miR-29b-1, miR-29a, or knockdown of miR-29b-1 and miR-29a in respective samples. The Truseq Stranded mRNA kit was used to prepare mRNA libraries from 2ug total RNA.  Libraries were confirmed on the Agilent 2100 Bioanalyzer and quantitated using the Illumina Library Quantification Kit, ABI Prism qPCR Mix from Kapa Biosystems and the ABI7900HT real-time PCR instrument.  75-76 cycle single read sequencing was performed with the 500 High-output v2 (75 cycle) sequencing kit on the Illumina NextSeq500 instrument.