GSM2501424: Cardiomyocytes-B3; Homo sapiens; RNA-Seq
Total RNA was extracted using a RNeasy plus Micro RNA kit (Qiagen) followed by RT-PCT to produce cDNA Libraries were prepared using the SMART-seq2 protocol modified to use Maxima H Minus enzyme for reverse transcription (Thermo Scientific) as follows: population controls were generated by extracting total RNA using a RNeasy plus Micro RNA kit (Qiagen) according to manufacturer’s recommendations, and 1 uL of RNA in water was added to 3 uL of elution mix.  Amplified cDNA products were purified using AMPure XP SPRI beads (Beckman Coulter) and eluted in TE buffer (Teknova). Cleaned-up amplified cDNA was used for library construction using the Nextera XT DNA Sample Preparation Kit and Nextera XT Index Kit (Illumina). Libraries were then pooled and cleaned up using AMPure XP SPRI beads (Beckman Coulter Genomics). Final library quality and quantity were assessed using a DNA High Sensitivity chip (Agilent).