GSM2501417: RCASA3; Gallus gallus; RNA-Seq
Basilar papillas were removed in HBSS and placed in QIAzol lysis reagent. Total RNA was extracted using the QIAGEN miRNeasy kit with an on-column DNase digest. The quality and quantity of the RNA was checked using an Agilent Bioanalyzer. An Illumina TruSeq Stranded kit was used to construct a cDNA library