Vehicle treated juvenile Macaca mulatta testis transcriptome
Total RNA was isolated from the entire right testes of each monkey  using Trizol reagent (Invitrogen) and RNAeasy columns (Qiagen). RNA sequencing was performed by the Genomics Research Core at the University of Pittsburgh as follows. Prior to library preparation, rRNA was depleted using the GeneRead rRNA Depletion Kit (Qiagen). Library preparation was performed using the Ion Total RNA-Seq kit v2 for Whole Transcriptomes (Life Technologies). Yield and size distribution were assessed by florometric assay and Bioanalyzer High Sensitivity DNA Chip. Barcoded libraries were pooled and diluted for emulsion amplification on Ion Sphere™ Particles (ISPs) using the Ion One Touch 2 System (Life Technologies). Templated ISPs were loaded onto the P1 semiconductor sequencing chip. Sequencing reaction and data collection were performed on the Ion Proton instrument.
Library preparation was performed using the Ion Total RNA-Seq kit v2 for Whole Transcriptomes (Life Technologies).