GSM1974450: monosome riboseq replicate 1; Saccharomyces cerevisiae; OTHER
Clarified lysate was separated on a sucrose gradient.  Monosome fractions were pooled, digested with RNaseI, and separated on a 2nd sucrose gradient.  Monosome fractions were collected and the RNA was extracted and size selected for ribosome footprints. Collected cells were resuspended in lysis buffer and lysed by vortexing with glass beads. RNAs were ligated to a preadenylated DNA linker and reverse transcribed.  cDNAs were gel purified, circularized, and PCR amplified.