GSM2774833: R01-043; Homo sapiens; RNA-Seq
Following surgical excision of tumor, necrotic debris and sampled cavitary lesions were removed to include a sufficient solid component.  Next, the surgeon cut a 3-5-mm-thick slice along the longest axis of the excised tissue, which was frozen within 30 minutes of excision and later retrieved for RNA extraction For library preparation, the TruSeq Total Stranded RNA with Ribo-Zero Reduction (Illumina) was used following manufacturer's instructions.