GSM1783229: DAY2; Acropora millepora; RNA-Seq
Total RNA was extracted from the samples using Trizol Reagent (Invitrogen) and dissolved in RNAse free water. RNA quantity and quality were assessed using a NanoDrop ND-1000 spectrometer and denaturing gel electrophoresis. Before being shipped on dry ice to the Macrogen sequencing facilities in Seoul, South Korea, each RNA sample was precipitated in ethanol and sodium-acetate (2x and 0.1x sample volume respectively), and stored at -80°C. In accordance with Macrogen procedure and Illumina Hiseq 2000 requirements