GSM3762575: D11_LEC_AD2_2h; Homo sapiens; RNA-Seq
Original cell extraction had been done by the commercial vendor (Promocell). Treated cells were stored in RNAprotect (Qiagen) before RNA was extracted with the NucleoSpin RNA kit (Macherey Nagel). Library preparation was done according to Illumina TruSeq Stranded mRNA Sample Preparation Guide. For data extraction, the base calling was performed using Illumina's standard bcl2fastq2 software (automatic adapter trimming). Stranded mRNA Sequencing. 1 x 50 bp read length was used, followed by 8 + 8 bp dual index run. The raw read per sample was 13-15M.