GSM4010730: GPD_miseq_BK724; synthetic construct; OTHER
PCR amplicons from random oligonucleotide pools, joined by Golden Gate assembly at constant regions encoding transcription factor binding sites based on the PGPD promoter. After FACS-sorting and overnight regrowth in the same media used for growth, sorted fractions were miniprepped. PCR was used to verify that at least 10 plasmids were extracted per sorted cell; minipreps were PCR-amplified, further amplified with the addition of bin-specific barcodes, pooled, and amplified briefly again.