GSM4011045: rancRNA_18_polysome-associated mRNA_rep1; Saccharomyces cerevisiae; RNA-Seq
Yeast spheroplasts were electroporated with or without RNA, recovered and harvested by centrifugation. Pellets were opened using a FastPrep. Lysates were cleared by centrifugation and loaded on a density gradient for Ultracentrifugation. Fractions containing polysomes were pooled and RNA was extracted via hot acid phenol extraction. Poly-A-Enrichment via oligo-dT-beads. PolyA enriched mRNAs were transformed into a cDNA library using the Illumina TruSeq stranded mRNA Kit. Library size was determined using Qbit.