RNA-Seq of Glycine max: root
Illumina sequencing was performed at Otogenetics. RNA was extracted from the pellets using Qiagen RNeasy Plant Mini Kit (50) Cat No.: 74904. The integrity and purity of total RNA were assessed using Agilent Bioanalyzer and OD260/280 using Nanodrop. cDNA was generated using Clontech SMARTer cDNA kit (Clontech Laboratories, Inc., Mountain View, CA USA, catalog# 634925) from total RNA. cDNA was fragmented using Bioruptor (Diagenode, Inc., Denville, NJ USA), profiled using Agilent Bioanalyzer, and subjected to Illumina library preparation using SPRIworks HT Reagent Kit (Beckman Coulter, Inc. Indianapolis, IN USA, catalog# B06938). The quality and quantity and the size distribution of the Illumina libraries were determined using an Agilent Bioanalyzer 2100. The samples were then sequenced on an Illumina HiSeq2500 which generated paired-end reads of 106 nucleotides (nt).
Index40_OtB0534