GSM3703559: C29_2_1; Homo sapiens; RNA-Seq
Cells were thawed and RNA was isolated from 300,000 CD27+CD70- and CD27-CD70+ Treg cells. Each experiment was performed in triplicate. mRNA capture and cDNA synthesis were performed using KAPA mRNA Hyper Kit for Illumina platform (Roche) according to the manufacturer's protocol. After quality check using Qubit High-sensitivity kit (Life Technologies), 16 cycles of PCR were carried out for amplifying each library. Amplified cDNA libraries were sequenced on an Illumina HiSeq4000 machine using paired end reads (2 x 150 bp) at Novogene, China.