3'-Seq on human failing heart: Sample DCM2
RNA was heat-fragmented to 100 nt fragments and polyA RNA was selected. An P7 adapter was ligated to the 5'end and RNA fragments were converted to cDNA using an achored oligo(d)T stem-loop primer (containing barcoded Illumina adapter). cDNA was amplified with primers P7 and Illunima_Truseq for 12 cycles before being sequenced on Illumina HiSeq2000 system
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