RAW-Ipr1(+H37Ra)-miRNA
The RAW-Control and RAW-Ipr1 cells were infected with H37Ra (MOI = 5) for 24 h (named RAW-Control (+H37Ra) and RAW-Ipr1 (+H37Ra) respectively), and uninfected RAW-Control and RAW-Ipr1 cells were used as controls. Three independent experiments were performed to prepare each sample. The total RNA was isolated using Trizol reagent (Invitrogen). RNA integrity was checked by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA). For each sample, equal amounts of total RNA from three independent experiments were pooled. For RNA-seq, the total RNA samples were treated with DNase I to degrade any possible DNA contamination. For small RNA sequencing, the sRNA (18 to 30 nt) was gel purified and ligated to the 3' and 5' adaptor. The ligated products were reverse transcribed, followed by acrylamide gel purification and PCR amplification to generate sRNA libraries. The library was loaded on an Agilent Technologies 2100 Bioanalyzer to check size, purity, and concentration.