Pyrosequencing of 16S rRNA gene amplicon obtained from the pooled HTC cultures showing cell densities of 10^4 to 10^5 cells per mL
A surface seawater sample collected from the East Sea was used for high-throughput dilution-to-extinction culturing of marine bacteria. After incubation, culture wells (of multiwell plates) were grouped into 3 sets according to cell densities. This sample was generated by pooling the cultures that showed cell densities of 10^4 to 10^5 cells per mL. The pooled cultures were filtered using 0.2-um pore size membrane filter. Total DNA was extracted from the filter and used for amplification of 16S rRNA gene (V1-V3 region; 27f and 519r primers). Amplified DNA was sequenced using 454 GS FLX Titanium platform.
ESS_HTC_4_to_5