GSM4009035: wildtype - Hyli IP; Hydra vulgaris; ncRNA-Seq
Trizol (Invitrogen) NEXTflex Small RNA-Seq Kit v3 (PerkinElmer Cat# NOVA-5132-05), The manufacturer's protocol was followed, with modifications to 3' and 5' adapter ligations: 3' adapter ligation was performed at 16°C overnight and 5' adapter ligation was performed at 20°C for two hours. Following adapter ligation, libraries were amplified for 18 cycles and were selected for a size of 108-180 base pairs using BluePippin (Sage Science).