GSM3103900: D39STP_2; Streptococcus pneumoniae; RNA-Seq
Total RNA was extracted using  Norgen RNA isolation kit (canada). The high quality total RNA samples were obtained by DNase treatment. The samples were then sent to BGI Americas. rRNA was removed from the total RNA samples.cDNA was then made using mRNA as template. Short fragments were made by heat treatment and the they  were purified and resolved  with EB buffer for endrepairation and single nucleotide A ( adenine) addition. Short fragments were then connected to adapters. Subsequently, following agarose gel electrophoresis, the suitable fragments were selected for the PCR amplification. All quality control (QC) steps i.e. for quatification and quality of sample library ABI bioanalyzer and ABI StepOnePlus realtime PCR system were used. Library were sequenced using IlluminaHiseq 2000.