GSM2160130: H3K27Ac_ChIP; Mus musculus; ChIP-Seq
FACS sorted BM pre-B cells (7-10 moths old) were washed and crosslinked 10 minutes at room temperature with 1% formaldehyde in PBS plus protease inhibitor (PI). Glycine was added at a final concentration of 125 mM and tubes were further mixed for 5 min at room temperature. ChIP reactions were performed with the Diagenode True MicroChIP X 16 kit as the manufactur's instruction. ChIP-Seq library preparation was carried out with the NuGen Ovation Ultralow System V2 1-16. Library amplification was performed for 15 cycles. All library preparation steps were carried out as stated in the user manual.