GSM1541799: 38-Lg_SFM_rep2 [RNA-Seq]; Homo sapiens; RNA-Seq
For RNA-seq, RNA was extracted with Trizol reagent. RNA-Seq libraries were generated using TruSeq Stranded mRNA protacol (Illumina). 2ug of total RNA was used for library generation and 6 minutes of fragmentation. For enrichment of cDNA fragments 11 PCR cycles were used. 