GSM1973935: xylulose evo 8a; Saccharomyces cerevisiae; RNA-Seq
Log phase cells were harvested and frozen in liquid Nitrogen. Total RNA was extracted using a NucleoSpin® 96 RNA kit (Macherey-Nagel), substituting β-mercaptoethanol with DTT RNA extracts were fragmented and polyadenylation was selected using PolydT beads. cDNA was prepared using Smartscribe and UMI-containing barcoded primers, and subjected to RNnase H treatment. A second adaptor was subsequently ligated.