GSM2350308: mouse.JNK.epithelium.susp.rep2.0; Mus musculus; RNA-Seq
RNA was extracted from cultured kidney epithelial cells using the RNeasy Mini Kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions.  On-column DNA RNAse-free DNase digestion was performed to eliminate genomic DNA.  RNA quality was verified (RNA integrity number >9) using a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). Messenger RNA was isolated, converted to cDNA, and amplified using the mRNA Purification Kit (Illumina, San-Diego, CA) according to the manufacturer’s instructions. An Illumina library was constructed and run on a HiSeq 2000 (Illumina).