U2AF35 siRNA, U2AF35 wt rescue, replicate 1
Total RNAs from cont siRNA and U2AF35 siRNA transfected cells as well as Q157R, Q157Rdel and Q157P rescue cell lines were purified using RNA-Tri (Bio&SELL) and further purified using the RNeasy mini kit (Qiagen) in combination with a DNase I (Qiagen) treatment to prevent genomic DNA contamination. RNA sequencing libraries were prepared by using the TruSeq mRNA Library Preparation kit (Illumina). 125-bp paired-end reads were generated by using a HiSeq 2500 sequencer (Illumina) with V4 sequencing chemistry. Biological duplicates were sequenced for all conditions.
siU35-wt_1