GSM1811346: wt_input; Drosophila melanogaster; ChIP-Seq
To identify KDM5 binding sites, ChIP-seq was carried out using HA-tagged KDM5 genomic rescue transgene flies with anti-HA (with or without paraquat treatment for 6 hours). Wildtype w1118 adults were used for  KDM5 and anti-H3K4me3 ChIP-seq. Libraries were prepared using Tru-Seq adaptors and then size-selected (150 bp-350 bp) and purified by agarose gel