GSM3259301: RNA-seq_Trachea Cells, before transplantation, bulk; Rattus norvegicus; RNA-Seq
Total RNA was prepared using the RNeasy Mini Kit (QIAGEN Inc.) and the RNase-Free DNase Set (Qiagen Inc). RNA-seq libraries were prepared using 400 ng of total RNA and the Illumina TruSeq Stranded mRNA reagents (Illumina; San Diego, California, USA) on a Caliper Sciclone liquid handling robot (PerkinElmer; Waltham, Massachusetts, USA) using a Caliper-developed automated script.