GSM4633811: Hum d15 batch 1; Homo sapiens; RNA-Seq
Samples were flash-frozen in LN2 and stored at -80 °C. RNA from all 42 samples was purified in parallel directly from Trizol with the Direct-zol-96 RNA kit (Zymo Research). cDNA libraries were generated according to NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760). cDNA was amplified for 12 cycles and barcoded with NEBNext Multiplex Oligos for Illumina (NEB #E7600). Samples were pooled together to 15 nM concentration and sequenced with single-end 50 base mode on 3 lanes of an Illumina HiSeq 4000 instrument. bulk RNAseq