chicken  manure digastate
DNA was extracted  using the cetyltrimethyl ammonium bromide (CTAB) / sodium dodecyl sulfate  (SDS) method and purified by 1% agarose gel. DNA was diluted to 10 ng/L and  used in PCR for amplification of archaeal  16S rRNA genes ,the Primers are  524F (5-TGY CAG CCG CCG CGG TAA -3) and958R (5-YCC GGC GTT GAV TCC AAT T -3).
B_Minhe1_2