GSM4084552: MiV_HET_FBN1; Mus musculus; RNA-Seq
Mitral valve tissue was harvested and pooled from 8 hearts of each genotype in separate WT and FBN1Het samples.  RNA was isolated using NucleoSpin RNA/Protein extraction kit (Machery-Nagel) and amplified using the Ovation RNAseq system v2 (NuGEN). cDNA was generated using the Nextera XT DNA sample preparation kit. RNA libraries were prepared for sequencing using Illumina HiSeq 2500 system