GSM2844209: M17_7 [BS-seq]; Zea mays; Bisulfite-Seq
Genomic DNA was sheared by DNA Shearing Instrument (30W%,3sON,9sOFF，20min) . The sheared DNAs size were between 400-500 bp and verified by 1.5% agrose gel electrophoresis. The quantity of the sheared DNAs was measured by Qubit 2.0(Invitrogen). For each sample, 4μg of genomic DNA was used for bisulfite-seq library preparation. Sheared DNAs were denaturated at 98℃ followed by bisulfite conversion. Then DNA was synthesized with DNA synthesis primer harboring tagging sequence. The DNA was purified and amplified and PCR products corresponding to 300-500 bps were purified, quantified and stored at -80 ℃ before sequencing. For high-throughput sequencing, the libraries were prepared following the manufacturer's instructions and applied to Illumina Hiseq 2000 system for 101 nt pair-end sequencing by Majorbio. Inc (Shanghai, China).