GSM3762915: Bt_larva_ATACseq; Bombus terrestris; ATAC-seq
Bombus terrestris workers at different developmental stages (egg, larva, pupa, adult) were collected and flash-freezing from several colonies to ensure the piled size of each sample set as one adult bee. The nuclei were extracted from homogenized frozen tissues and then used to react with Tn5 transposase. After incubation, the transposed DNA were eluted and purified to run PCR reaction. The detailed procedures of amplification and following purification were used based on previous ATAC-seq protocols (PMID: 24097267, PMID: 25559105).