GSM2349429: RNA-seq Cbx7 rep 1; Mus musculus; RNA-Seq
Total RNA was Trizol-extracted from cells used for dCLIP experiments. Extracted RNA was depleted of ribosomal RNA using Ribominus kit (Life technologies). Strand-specific cDNA libraries were constructed using Superscript III reverse-transcriptase for first-strand synthesis, NEBNext mRNA Second Strand Synthesis Module supplemented with dUTP (NEB) for second-strand synthesis, and NEBNext ChIP-Seq Library Prep Master Mix Set for library preparation.Multiplexed library, Ilumina barcode#6, GCCAAT. Single-end 50bp reads