Immune repertoire-seq of canis familiaris: right popliteal lymph node with TZL & BCL - tech rep 1
Genomic DNA was extracted from scraped cytology slides of the patient's lymph node; the lymphocyte antigen receptor loci TRB, TRG and IGH were amplified by multiplex PCR using primers targeting the V/J genes; this is technical replicate 1/2; library preparation was done using the NEB Ultra II kit as per manufacturer's instructions.
17-063145.1-1