GSM4634808: mESCs_WT_N2B27_D6; Mus musculus; RNA-Seq
ESCs were collected and RNA was harvested using Trizol reagent. Oligo(dT)-attached magnetic beads were used to purified mRNA. Purified mRNA was fragmented into small pieces and then cDNA was generated using random hexamer-primed reverse transcription and amplified by PCR for construction of sequencing libraries.