GSM3703200: mrna-seq G1; Takifugu fasciatus; RNA-Seq
Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer's protocol. The total RNA quantity and purity were analysed with a Bioanalyzer 2100 and an RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number > 7.0. For constructing six cDNA library, approximately 5 μg of total RNA per sample was used for the RNA sample preparations. The library for sequencing was generated using an Illumina TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA, USA). Transcriptome sequencing was carried out on an Illumina HiSeq 2500 platform that generated approximately 125-bp paired-end (PE) raw reads by LC Sciences (Houston, TX, USA). After removing adaptor sequences, ambiguous 'N' nucleotides (with the ratio of 'N' greater than 5%) and low quality sequences (with quality score less than 20), the high-quality trimmed sequences were used for further mapping to the T. fasciatus genome (unpublished data) with Hisat 2.0.4.