GSM2349431: ChIP-seq Control; Mus musculus; ChIP-Seq
Cells were crosslinked with 1.1% formaldeyde for 20 min, then nuclei were isolated and lysed. Chromatin was sheared by sonication to a size of 100-500bp. Cleared lysates were incubated overnight with anti-HA tag antibody, with 10% saved as input, then captured with Dynabeads Protein G. After stringent washes, ChIP DNA was eluted from beads, and together with input chromatin was reverse-crosslinked on 650C and treated with proteinase K. DNA was purified by phenol/chloroform extraction and ethanol precipitation. ChIP-seq libraries were constructed using the NEBNext ChIP-Seq Library Prep Master Mix Set (NEB). Multiplexed library, Ilumina barcode#1, ATCACG. Paired-end 50bp reads