16S amplicon-Seq of Trichodesmium bloom biomass 6h after incubation, bottle100
amplified with conserved bacterial primers 27F and 1100R (Dowd et al., 2008) using a high fidelity polymerase (Phusion DNA polymerase, Thermo Scientific), second PCR with fusion primers containing individual tags per sample; AMPure magnetic purification; fragment size for sequencing ~340 nt
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