GSM1971608: Mouse chondrocyte pellet culture in the presence of pterosin B, sample 5; Mus musculus; RNA-Seq
RNAs was extracted from these cultured chondrocyte pellets using QIAzol Lysis Reagent (Qiagen) and the miRNeasy Mini Kit (Qiagen). RNAs were also extracted from various mouse tissues. One hundred (rib cartliage) or two hundreds (the others) ng of total RNA was subjected to library preparation using KAPA stranded mRNA-seq Kits (KAPA Biosystems, Woburn, MA), according to manufacturer’s instruction. The libraries were sequenced in 101 cycle Single-Read mode of HiSeq2500.