GSM3103145: IMR_DMSO_3; Homo sapiens; RNA-Seq
RNA extraction was performed with TRIzol (Ambion) following the manufacturers' instructions. Total RNA was treated with DNase I (Invitrogen). Subsequently, the purified RNA was fragmented on a BioRuptor Next Gen (Diagenode) at high power for one cycle of 30''/30'' ON/OFF. Sequencing libraries were prepared with the RNA-seq library kit (TruSeq Stranded Total RNA RiboZero Gold, Illumina)