GSM4082890: Mound stage replicate 2; Dictyostelium discoideum; ATAC-seq
Frozen pellets of D discoideum cells from the various stages were washed twice with cold PBS and lysed with 10 mM Tris-HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl2, 0.1% Igepal-630 Nuclei were transposed using the Nextera DNA Library Prep Kit.  Transposed libraries were amplified for 11 cycles and size selected for fragments ranging 200-1000 bp. ATAC-seq experiments were sequenced to a minimum depth of 10 million reads/sample.