DNA-seq of Arabidopsis thaliana :seedling
After the genomic DNA test of the sample is qualified, the DNA is fragmented by mechanical interruption (ultrasound), and then the fragmented DNA is subjected to fragment purification, end repair, A addition at the 5 end, sequencing adapter connection, and then agarose gel Electrophoresis is used to select the size of the fragments, and PCR amplification is performed to form a sequencing library. The library is constructed and then subjected to library quality inspection. Illumina is used to sequence the qualified library.
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