GSM1642336: Pch2; Phaeomoniella chlamydospora; RNA-Seq
RNA was extracted using Qiagen RNeasy Plant Mini Kit and RNase-Free DNase Set for complete removal of genomic DNA. RNA was reverse transcribed using Biorad iScript™ cDNA Synthesis Kit and specific transcripts were verified using qPCR (iQ™ SYBR® Green Supermix, Biorad ). Total RNA samples were further processed by VetCORE, VetMed University Vienna for high throughput sequencing. RNA fragmentation and Reverse Transcription were realized according to TruSeq RNA prep Kit v2 manual (Illumina, San Diego, US) and 50bp single end sequencing was performed using HiSeq V4 Illumina sequencer.