GSM3259240: mass_M1 miRNA seq; Coryphaena hippurus; miRNA-Seq
miRNAs of pooled samples were isolated using the miRNeasy mini kit from Qiagen (Valencia, CA).  The quality and concentration of miRNA were determined using the Agilent 2100 small RNA chip (Santa Clara, CA). miRNA libraries were made using the New England Biolabs NEBNext Multiplex Small RNA Sample Prep kit (Ipswich, MA) following the manufacturer's protocol. The size distribution and concentration of the libraries were determined using the Agilent High Sensitivity DNA Assay Chip (Santa Clara, CA).