"DNA-Seq of Agave tequila: Mate-pair, 15kb"
"Nuclear DNA for library preparations was extracted from young leaf as described (Carrier et al. 2011. Am J Bot 98: e13-15) with minor modifications. The mate-pair library with an average insert size of  15 kb was made using the Nextera Mate Pair Sample Prep Kit (Epicentre, WI, USA ) and sequenced on a Miseq instrument (Illumina, CA, USA) using MiSeq Reagent Kit v2 (500-cycle). The reads were trimmed and filtered using Trimmomatic (Bolger et al. 2014. Bioinformatics. doi: 10.1093/bioinformatics/btu1170) on the following criteria: (1) a Q20 quality cutoff; and (2) minimum read length of 40 bp."
Leaf_MP_15kb