GSM2159954: RNA 4h glucose B; Rattus norvegicus; RNA-Seq
Following Isol-RNA lysis Reagent® (5-Prime) extraction and EconoSpin (Epoc Life) column purification of total RNA, contaminant genomic DNA was removed from purified total RNA by TURBO® DNase digestion (Life Technologies), and ribosomal RNAs were removed using the Ribo-Zero® Human/Mouse/Rat kit (Epicentre). RNA fragmentation and cDNA synthesis was performed according to the manufacturers (Truseq 2® , Illumina) instructions. RNA-, DNase-, and ChIP-seq libraries were constructed using PentAdapters (Pentabase) essentially as previously described in (Nielsen R, Mandrup S, 2014, Methods in Enzymology 2014, Vol. 537, pp. 261-279).