GSM4083492: 25: Metastases1; Danio rerio; Homo sapiens; RNA-Seq
Metastasis samples in zebrafish embryos and uninjected control were isolated by cutting the whole metastatic area (80 fish per group randomly chosen from >100 engrafted fish) at 6dpi (8dpf) with a micro dissection scissor (WPI). The samples were immediately washed with cold PBS and stored in TRIzol (Sigma) at -80 ˚C. The whole process was finished within 30 min.  1000000 Cells in culture were lysized with TRIzol and stored at  -80 ˚C. RNA isolation was further performed using RNeasy mini kit (qiagen) then Illumina RNAseq libraries were prepared using the Illumina TruSeq RNA Sample Prep Kit v2 according to the manufacturer's instructions (Illumina, San Diego CA, USA).