GSM4010837: HiC_Retina_E14.5-Rep1; Mus musculus; Hi-C
In situ Hi-C protocol was performed using retinal cells, with minor modification to the original protocol (Rao et al., 2014).  Two to five million cells were crosslinked with 1% formaldehyde for 10 min at room temperature, then digested with 125 units of MboI overnight followed by labeling with biotinylated nucleotides and proximity ligation. After reverse crosslinking, ligated DNA was purified and sheared to 300-500 bp using a Covaris LE220. The DNA fragments containing ligation junctions were pulled down with streptavidin beads followed by Illumina compatible library construction and paired end sequencing.