RNA-seq of Dromaius novaehollandiae: female spleen
RNA extraction was performed using RNeasy Plus Mini kit (Qiagen). Poly-A selection was conducted on the total RNA using PrepX PolyA mRNA Isolation Kit (Takara). The mRNA was assessed using the RNA Pico kit (Agilent) and used to make transcriptome libraries using the PrepX RNA-Seq for Illumina Library Kit (Takara). The libraries were quantified by performing qPCR (KAPA library quantification kit) and then sequenced on an NextSeq instrument (High Output 150 kit, PE 75 bp reads)
emu-5F-spleen