GFP-IP
RNA from Sindbis-virus-infected Aag2 cells was isolated from V5 immunoprecipitates (V5 was tagged to the indicated protein). This RNA fraction was used as input for deep sequencing library preparation using the Truseq small RNA sample preparation kit (Illumina). The library was sequenced on an Illumina HiSeq2500 by Baseclear (Leiden, The Netherlands).
GFP-IP