plasmid sequencing of Bacillus thuringiensis
The DNA was normalized to 1000 ng in the volume of 130 l.The sample was subjected to mechanical shearing using microTUBE AFA Fiber Snap- Cap tubes on M220 Focused-ultrasonicator.The DNA was targeted for the fragments in the range of 250 bp to 300 bp using Ampure purification.The fragmented DNA molecules were taken for whole genome DNA library preparation using NEBNext UltraTM II DNA Library Prep kit (E7645L) by following the standard protocol is not present in your account, so use the correct sample_name or BioSample accession. If someone else in your group created the BioSample, then use the accession in this table. If the BioSample belongs to a different group, then you need to create a new BioSample
IVBT62pDNA_1