GSM3763073: N14_4; Mus musculus; RNA-Seq
Frozen tissues were crushed with a pestle and the tissue lysates homogenized. Total RNA was isolated (RNeasy Mini Kit, QIAGEN) following the manufacturer's protocol, depleted of ribosomal RNA via a hybridization method (Ribo-Zero Gold rRNA Removal Kit, Illumina), and purified with DNase treatment (Ambion DNA-free DNA Removal Kit, Thermo Fisher Scientific). RNA libraries were prepared for sequencing using 1 µg of total RNA per sample (average RNA integrity number of 8) and standard Illumina protocols.