GSM2915991: REP1-TEAD1-ChIPSeq-WT-S; Homo sapiens; ChIP-Seq
Lysates were clarified from sonicated and enzyme digestion nuclei and histone-DNA complexes were isolated with antibody. Vazyme ND604 kit was used for ChIPSeq  library construction. Brifely, about 10-100ng dsDNA was used as initiation for end repair and prepared for adaptor ligation. After adaptor ligation, PCR amplification with 8-13 cycles for each library and gel selection was used for library size selection and purification.