ITS amplicon of environmental soil:bulk: Sample sample_myco21
"DNA extracts were amplified in double with ITS3 and ITS4 ITS rRNA bacterial primers comprising the ITS2 hypervariable region. PCR products were pooled, purified with PureLink PCR Purification Kit and quantified with Qubit 2.0 Fluorometer. Libraries were prepared using the Nextera XT kit, purified using AMPure XP beads, normalized, pooled and sequenced using the Illumina MiSeq 2x250bp paired-end chemistry."
library_myco21