GSM1541530: FLAG::hrde-1_input; Caenorhabditis elegans; ncRNA-Seq
Total RNA from cell lysates or FLAG::HRDE-1-bound RNA was isolated by Trizol extraction followed by two rounds of chloroform extractions and precipitated using isopropanol. Small RNAs were size selected, tobacco acid pyrophosphatase (TAP) or RNA 5' polyphophatase treated to remove 5’ di- and triphosphate groups, ligated to 5’ and 3’ adapters, subjected to reverse transcription, amplified by PCR, and gel purified in preparation for sequencing.