GSM4633908: SPS2; Rattus norvegicus; RNA-Seq
We extracted total RNA from the hippocampi of rats using TRIzol reagent. Ribo-Zero rRNA Removal Kits were used to remove the rRNA from total RNA. RNA libraries were constructed by using rRNA-depleted RNAs with TruSeq Stranded Total RNA Library Prep Kit. Quality and quantity of the libraries were controlled by using the BioAnalyzer 2100 system. We denatured 10 pM libraries as single-stranded DNA molecules, captured on Illumina flow cells, amplified in situ as clusters, and finally sequenced for 150 cycles on an Illumina HiSeq Sequencer. RNA libraries were prepared for sequencing using standard Illumina protocols