GSM1908882: WW_unc_12_3; Schmidtea mediterranea; RNA-Seq
To isolate stem cells, we used a well-established method to isolate planarian stem cells by Hoechst blue staining and flow cytometry. Sorted cells were homogenized in Trizol reagent and RNA was isolated per the manufacturer-supplied protocol. Isolated RNA from whole worm tissue was treated with RNase-free DNase on QIagen RNeasy columns and eluted in nuclease-free water. 500ng-1μg RNA per whole worm sample or 100ng of sorted cell RNA was used for generation of RNAseq libraries using the Illumina TruSeq kit.