GSM3702151: S1; Arabidopsis thaliana; RNA-Seq
RNA was extracted from 1-cm stem segments including the stem base. Three stem segments were pooled into one biological replicate. Tree biological replicates were collected per genotype. RNA was extracted by phenol/chlorophorm as described (Mallory and Vaucheret 2010, PlantCell) with the modifcation of two additional concluding 70% EtOH washes. 2 µg of total RNA were treated by DNase (Fermantas) and sumbitted for library preparation to the Deep Sequencing Core Facility provided by the Exzellenzkluster CellNetworks at the University of Heidelberg (http://www.cellnetworks.uni-hd.de/483065/Deep_Sequencing_Core_Facility). RNA libraries were generated using the NEBNext Ultra II Directional RNA Library Prep kit with NEBnext Multiplex Oligos for Illumina. For Poly A selection the NEBNext Poly A selection module was used. RNA fragmentation time was 15 minutes, and 12 cycles of PCR were used for amplification. All library preparation steps were executed by the Deep Sequencing Core Facility provided by the Exzellenzkluster CellNetworks at the University of Heidelberg (http://www.cellnetworks.uni-hd.de/483065/Deep_Sequencing_Core_Facility)