GSM1423130: SIM 64; Homo sapiens; RNA-Seq
RNA was extracted using the Qiagen RNA minElute kit (Qiagen, Limburg, Netherlands) with slight modification of the manufacturer’s protocol utilising TRIzol reagent. mRNA purification, cDNA synthesis, Adapter ligation, DNA enrichment, library validation and normalisation were performed with the TruSeq RNA kit (Illumina inc. San Diego, CA) as per the manufacturer’s instructions (sample prep v. 2).