GSM2773056: sgGFP, biological rep1; Mus musculus; RNA-Seq
RNA was isolated from cells using RNeasy Kit (Qiagen). Libraries were prepared using Illumina TruSeq Stranded mRNA sample preparation kits from 500ng of purified total RNA according to the manufacturer’s protocol. The finished dsDNA libraries were quantified by Qubit fluorometer, Agilent TapeStation 2200, and RT-qPCR using the Kapa Biosystems library quantification kit according to manufacturer’s protocol. Uniquely indexed libraries were pooled in equimolar ratios and sequenced on a single Illumina NextSeq500 run with single-end 75bp reads by the Dana-Farber Cancer Institute Molecular Biology Core Facilities.