GSM4082101: Hum_serum_small; Homo sapiens; miRNA-Seq
Total liver RNA and exosome RNA were isolated with Norgen total RNA purification kit according to kit's protocols for each type of samples (Norgen Biotek Corp., Canada), small RNAs were enriched using Ion Total RNA-Seq Kit v2 (Life Technologies). 2 µg total RNA from the pool of 3 sampes per each condition was depleted for ribosomal RNA with RiboMinus Eukaryote System v2 (cat # A15026, Ambion, ThermoFisher Scientific), transcriptome RNA and small RNA  libraries were prepared with Ion Total RNA-Seq Kit v2 according to the kit manual