O.latipes Adult_2 RNA-seq
RNA was isolated from FACS sorted gonadotropes from Gfp-transgenic medaka. The Ovation RNA-seq System V2 (NuGEN) was utilized and the cDNA was fragmented (~300 bp) before employing 10 cycles of PCR using Phusion polymerase. Samples were subjected to paired end sequencing with a read length of 100 nucleotides on an Illumina HiSeq2000.