pooled forest soil sample-S216
"40 pooled samples (50 mm diam to 5 cm depth) from 2500-m2 plot; 2 g DNA extracted using MoBio Soil Maxi kit; PCR targeting ITS region of fungi using MID-tagged primers (fwd:CTAGACTCGTNRNCGATGAAGARCGNNG,rev:MID-TCCTSCGCTTATTGATATGC); Exo-SAP purification, SequalPrep Normalisation; adapter ligation; emPCR"