ChIP-Seq of Ciona intestinalis, Whole Embryo, e. gastrula stage, WT, rep2, input chromatin
For each sample, embryos batches were snapfrozen in liquid nitrogen at the early gastrula stage and kept at -80C. After crosslinking with 16% formaldehyde for 10 minutes at room temperature, chromatin was sonicated with a Bioruptor to an average size of 200bp. An aliquot of the sonicated chromatin was reverse-crosslinked and a library constructed with an Illumina kit was constructed. Sequencing (50SE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China).
Ciinte-WE-eGast-WT-R2-Input