GSM3258613: RNA-seq_Act.5 h + GFP_rep2; Homo sapiens; RNA-Seq
Total RNA was isolated with the Aurum™ Total RNA Mini Kit (BIO-RAD, cat#7326820) including on-column DNaseI digest. PolyA selection and fragmentation of RNA were performed according to the TruSeqTM Stranded polyA RNA Sample Preparation Guide from Illumina. The fragmented RNA was subjected to first-strand cDNA synthesis. For second-strand cDNA synthesis and adapter ligation, the IP-Star® Compact automation system was used. After that, library construction was performed according to the Illumina protocol.