Interleukin-17 Promotes Neutrophil-Mediated Immunity by Activating Microvascular Pericytes and Not Endothelium
Human placental PCs were stimulated with 20ng/ml of IL-17 (R&D), TNF (Gibco), or both for 12 hours. Total RNA was purified using the RNeasy Mini kit (QIAGEN), in which an on-column DNase treatment was included. Purified RNA was submitted to the Yale Center for Genomic Analysis where it was subjected to mRNA isolation and library preparation. Libraries were pooled, 6 samples per lane, and sequenced on an Illumina HiSEQ 2500 (75bp paired end reads), and aligned using STAR to the GRCh37 (hg19) reference genome.
Sample_RL446-12_012