GSM2772525: IMR90 WGBS; Homo sapiens; Bisulfite-Seq
NA12878 genomic DNA was purchased from ATCC. IMR90 high molecular weight genomic DNA was extract from cultured cells using SDS+proteinase K lysis/phenol-chloroform treatment. Extracted DNA was treated DNase-free RNase A. 100 ng of genomic DNA was treated with Tn5 transposase for tagmentation, then tagged DNA was filled with methylated dCTP, dATP, dGTP, dTTP mixture. The filled DNA was bisulphite treated using EZ DNA methylation Gold kit (Zymo research). The bisulphite treated samples were amplified with custom and Illumina index adapters. The amplified products were size selected using AMPureXP beads.