GSM2500268: Cerebral Cortex from WT mice; Mus musculus; RNA-Seq
Brains were removed, cerebral cortexes (Cx) and midbrain/diencephalon/hippocampus (M) areas were isolated, placed overnight at 4°C in RNA later Tissue Collection - RNA Stabilization Solution (Thermo Fisher Scientific) and stored at -80°C until RNA extraction. Total RNA was extracted using TRIzol Reagent (Thermo Fisher Scientific)  and quantified through NanoDrop 1000 Spectrometer (Thermo Fisher Scientific). Total RNA integrity was assessed through Agilent Bioanalyzer 2100 and RNA 6000 Nano Kit (Agilent Biotechnologies). mRNA was purified by using the Dynabeads mRNA Purification Kit (Thermo Fisher Scientific) according to the supplied protocol; mRNA enrichment was assessed by using the 2100 Bioanalyzer and RNA 6000 Pico kit (Agilent Biotechnologies). RNA libraries were prepared for sequencing using standard SOLiD Whole Transcriptome Analysis kit