GSM3104154: KI542_ATAC_BJ5ta; Homo sapiens; ATAC-seq
One hundred thousand trypsinized cells were incubated with ATAC hypotonic buffer (10 mM Tris pH 7.5, 10 mM NaCl, 3 mM MgCl2) at 4ºC for 10 min during 500 g centrifugation. Cells were incubated in Tagmentation mix (Tagmentation DNA buffer Illumina 15027866; Tagmentation DNA enzyme Illumina 15027865) at 37ºC for 30 min. Purified DNA was used to construct high-throughput sequencing libraries using NEBNext High-Fidelity 2x PCR Master Mix (New England Biolabs M0541).