GSM2774975: ATAC_NIS_Rep1; Homo sapiens; ATAC-seq
ATAC-seq performed using 100,00 cells and 13 cycles of PCR amplification. Otherwise, as previously described (Buenrostro et al., 2015). Libraries prepared using 13 cycles of PCR following nuclei transposition using the Nextera DNA library preperation kit (Illumina, FC-121-1030). Samples were size selected between 170 and 400bp (in order to isolate ‘nucleosome free’ and ‘mono-nucleosome’ fragments) using SPRIselect beads (B23319, Beckman Coulter)