ITS region of deadwood, sample W1b__2012
Amplicons were generated using the primers for ITS1F and ITS4 (Gardes and Bruns, 1993) to characterize fungi (5-CTTGGTCATTTAGAGGAAGTAA-3, 5-TCCTCCGCTTATTGATATGC-3)  GoTaq Flexi DNA polymerase and buffer (Promega; Madison, WI, USA; M8297 and M890), hereafter GoTaq, was used on samples collected in 2012.
W1b__2012ITS