Ab10 progenitor developing ear methylC, plant 2
DNA was extracted from 50 mg developing ear tissue (4-6 cm stage) using a DNeasy Plant Mini Kit (Qiagen; 69104). 2 μg of DNA was used for preparing each MethylC-seq library according to the method of Urich et al (2015) Nature Protocols (except that Mag-Bind® RxnPure Plus was used instead of AMPure XP beads for purification steps). Two independent PCRs were used to amplify the libraries, 5 cycles for SRR2649648 and 6 cycles for SRR5579273.
56L_methylC