GSM4569609: MV4-11-11_RNA seq; Homo sapiens; RNA-Seq
Total RNA was extracted from cells with the RNeasy Mini Kit Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA). The library fragments were purified with QIAQuick PCR kits (QIAGEN).The clustering of the index-coded samples was performed on a cBot cluster generation system using HiSeq PE Cluster Kit v4-cBot-HS (Illumina). After cluster generation, the libraries were sequenced on an Illumina platform and 150 bp paired-end reads were generated.