GSM2498512: Pdgfra-GFP positive cells E13.5 Brain 2; Mus musculus; RNA-Seq
Forebrain and spinal cord were excised and tissue was mechanical dissociated in HBSS with ions using 3 different Pasteur pipettes of decreasing diameter Variable, see sample Illumina’s TruSeq Stranded Total RNA Library Prep Kit was used according to manufacturer’s instructions. Three pooled biological replicates were sequenced for each developmental stage and brain region, and a total of 38 - 66 million 150 bp strand-specific paired-end reads were generated for each replicate, comprising a total of 620.5 million reads across all datasets