GSM2232186: A748; Homo sapiens; RNA-Seq
Total RNA was extracted from fresh-frozen bulk liver biopsy tissue using Trizol reagent (Invitrogen) and subsequently subjected to DNase treatment using DNA-free™ DNA Removal Kit (Ambion) according to manufacturer’s instructions. RNA concentration was determined using NanoDrop 2000 spectrophotometer (Thermo Scientific) and RNA quality/integrity was assessed in an Agilent 2100 bioanalyzer using RNA 6000 Nano Kit (Agilent Technologies). All 46 RNA samples included in this study had RNA integrity number (RIN) values of >7 (7.1-9.4; median 8.9), with 42 out of 46 samples having RIN values ≥8. RNA sequencing libraries were constructed with the TruSeq Stranded mRNA protocol, with polyA selection.