RNA-seq of Homo sapiens: Mixed phenotype acute leukemia : Sample TARGET-15-SJMPAL003414-04A.1-01D
Total RNA quality and quantity were assessed on Agilent RNA6000 chips and Qubit. RNA-seq libraries were prepared from 500 ng of total RNA for each sample following Illumina RNA-seq protocols, including DNase treatment and phenol purification, cDNA conversion, fragmentation by Covaris Ultrasonicator, end repair, deoxyadenosine tailing, adaptor ligation and PCR amplification (ten cycles). Libraries with a 10 pM concentration were clustered on an Illumina cBot, and each flow cell was loaded onto a HiSeq instrument for sequencing using the Illumina 2 x 100 bp sequencing kit.
SJMPAL003414_D2