B73_Nextera_H3K9me2_ChipSeq
H3K9me2 profiling was performed on three replicates of B73 14-day-seedling using antibodies specific for H3K9me2 (#07-441) purchased from Millipore (Billerica, USA) according to manufacturer’s recommendations. TruSeq-methylated adapters were ligated to the B73 DNA fragments according to the NEBNext DNA Library Prep protocol. The sample was then sequenced to greater than 100 million reads on the HiSeq 2000 (Illumina), single end.