GSM4010338: MUC3860_LS24_HP_RNA_Seq; Homo sapiens; RNA-Seq
700 ng of total RNA was isolated from liver tissue using NucleoSpin® RNA Kit (Macherey-Nagel, Düren, Germany) Libraries was prepared using the TruSeq Stranded mRNA Library Prep (Illumina, ‎San Diego, USA). RNA libraries were assessed for quality and quantity with the 2100 BioAnalyzer (Agilent technologies, Böblingen, Germany) and the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific, Waltham, USA). RNA libraries were sequenced as 100 bp paired-end runs on a HiSeq 4000 platform (Illumina, ‎San Diego, USA).