GSM3179300: H3K27Ac_HiChip; Homo sapiens; OTHER
DNA from ten million cells were crosslinked with 1% formaldehyde for 10 min at room temperature, digested with 375 units of MboI, labeled by biotinylated nucleotides and proximity-ligated. After sheared by Covaris, chromatin immunoprecipitated (ChIP) with H3K27Ac antibody (Active Motif AM, cat# 39133, lot# 8), the ChIP DNA was de-crosslinked and purified by Zymo Genomic GNA Clean & Concentrator kit. Finally, after streptavidin beads pull-down and Illumina library construction by Kapa Hyper Prep Kit, HiChIP library was sequenced paired-end 75 cycles on Illumina NextSeq 500.