Illumina HiSeq 2500 paired end sequencing
Exome capture
LIB15395
Genomic DNA was extracted from leaves of individual M2 adult plants. DNA was extracted using MagAttract DNA Blood M96 Kit (Qiagen) following the instructions provided by the supplier. The freeze-dried material was lysed with ammonium acetate and precipitated on to Agencourt Genfind v2 (Beckman Coulter_ A41497) magnetic beads and washed several times. The purified DNA was eluted into low-EDTA TE using the Beckman FXp robotic system and the samples were normalised to 20 ng/�L on a Beckman NX platform. Purified DNA samples were sheared on a Covaris E220 instrument. Genomic DNA libraries were constructed with a Sciclone G3 robotics (PerkinElmer) using the High-Throughput Library Preparation kits from KAPA Biosystems, Inc. (Wilmington, MA, USA, catalog number KK8234) following the Maestro KAPA HTP protocol with dual-SPRI bead size selection. The libraries were barcoded using Roche adapters � set �A� (1-12) (Roche, catalogue number 07141530001). During the library preparation step, samples were amplified by PCR, using six amplification cycles. The products were purified with Agencourt AMPure beads (Beckman Coulter, A63881) on the Sciclone G3 platform and were eluted in ultrapure DNase/RNAse free distilled water.