Germline WGS of Homo sapiens: colorectal cancer case-control: Sample 72258
Library construction was performed using the Rubicon Genomics Thruplex-FD kit and 500ng of genomic DNA.  Samples were sheared using a Covaris focused ultrasonicator targeting a mean insert size of 300bp.  Shearing was followed by a SPRI bead cleanup and enzymatic steps, which performed template prep and added adapters. Libraries were amplified by PCR using custom indexed primers and then subjected to a final SPRI bead cleanup. Prior to sequencing library quality was confirmed by fluorescent quantification and by Agilent 2100 Bioanalyzer profile. All libraries were sequenced on the Illumina HiSeq 2500 platform with PE100 reads following the manufacturer's protocols.
27-22251_G:12: 72258