Illumina HiSeq 2000 sequencing; RNA-Seq of YM155 treated KMB7 wild-type and SLC35F2 knock-out cells
RNA-Seq of YM155 treated KMB7 wild-type and SLC35F2 knock-out cells
SLC35F2_GT_1_DMSO
Cells were grown in IMDM media supplemented with 10% fetal bovine serum. Cells were seeded at a density of 0.5x10e6 cells/ml Cells have been treated with 1 uM YM155 for six hours. Control treatment has been conducted with DMSO as vehicle control with a final DMSO concentration of 0.01% DMSO in each condition. RNA has been isolated from 5x10e5 cells each using RNAeasy kit (Qiagen) and library preparation has been performed with TrueSeq RNA v2 kit (Illumina) RNA has been isolated from 5x10e5 cells each using RNAeasy kit (Qiagen) and library preparation has been performed with TrueSeq RNA v2 kit (Illumina)