Environmental partial nifH sequences from sample: ESP_Stn2_20m_S48811
Partial nifH sequences were amplified from environmental DNA using nested PCR with the universal primers of Zehr and McReynolds (1989) and Zani (2000). Samples were multiplexed using a one-step PCR with gene-specific sites, forward and reverse barcodes, Illumina linkers, and sequencing primer binding regions, similar to the method of Kozich et al. (2013). Though this was a paired-end run and both forward and reverse index reads were used for demultiplexing, only forward reads could be used for phylogenetic analyses due to the poor quality of reverse reads.
ESP_Stn2_20m_S48811_library