GSM3703650: Shenai3; Homo sapiens; miRNA-Seq
RNA of cells and exosomes was isolated using Trizol Plus RNA purification kit (Life Technologies, 12183555) according to manufacturer's protocol. The quality of the total RNA was assessed by Agilent 2100 Bioanalyzer using an RNA 6000 Pico Kit (Agilent Technologies). After verifying the quality of RNA, we proceeded to the second part of the protocol of small RNA enrichment from total RNA by increasing ethanol content of the sample and isolation over glass-fiber filter and elution. Later, quality of the small RNA samples was analyzed by Agilent 2100 Bioanalyzer using a Small RNA kit (Agilent Technologies). RNA total concentration was measured by Nanodrop, and miRNA concentration was measured with the Quant-iT RiboGreen Kit (Thermo-Scientific). RNA libraries were prepared for sequencing using standard Illumina protocols.