GSM2500582: S3 rep3; Brassica rapa subsp. pekinensis; RNA-Seq
Total RNA was isolated from fertile and sterile flower buds using TRIzol reagent according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA, USA). RNA quality and DNA contamination was monitored by electrophoresis on 1% agarose gels. RNA concentration was measured using the Qubit RNA Assay Kit and a Qubit 2.0 Flurometer (Life Technologies, Carlsbad, CA, USA). Finally, RNA integrity was assessed using the RNA Nano 6000 Assay Kit on the Bioanalyzer 2100 system. RNA libraries were prepared for sequencing using standard Illumina protocols.