GSM4008841: SHPC34 DUHi mX 196; Mus musculus; RNA-Seq
Following dissection in culture medium, tooth germs were stored in RNA later at -20°C. RNA was extracted with RNAeasy micro kit (Qiagen), and controlled with Q-bit (Invitrogen) and Tapestation (Agilent technologies). RNA-Seq samples were prepared following the TruSeq® RNA Sample Preparation v2 Guide, starting from 100ng of total RNA of top quality (RINe>9.5).