Human Triple-negative breast cancer sequencing
Massive parallel sequencing was performed using a custom panel of 100 genes. DNA was extracted using High Pure FFPET DNA isolation kit (Roche Diagnostics). Processing of samples for sequencing was done using Sure Select XT Target Enrichment System for Illumina Paired-end Multiplexed Sequencing Library (Agilent Technologies). Libraries were amplified by PCR and quality and quantity analysis were done using Bioanalyzer and DNA 1000 Assay (Agilent Technologies). gDNA libraries were hybridized with a target-specific Capture Library. The targeted molecules were captured with streptavidin beads (Dynabeads MyOne Streptavidin T1, Life Technologies). Samples were sequenced by a HiSeq 2.500 powerful high-throughput sequencing system
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