GSM2773066: control-1 [DuiA]; Xenopus laevis; RNA-Seq
Testes were removed, flash-frozen in liquid nitrogen,  stored at -80 C, and RNA was harvested using Trizol. The concentration and purity were measured using a NanoDrop® ND-2000 (Thermo, Waltham, MA, USA) spectrophotometer. The integrity of RNA was determined by denaturing gel electrophoresis  for the construction of sequencing libraries. RNA libraries were constructed by using rRNA-depleted RNAs with TruSeq Stranded Total RNA Library Prep Kit (Illumina, USA) according to the manufacturer’s instructions. Libraries were controlled for quality and quantified using  the BioAnalyzer 2100 system (Agilent Technologies, USA).