RNA-Seq of homo sapiens: adult female plasma
cfRNA was extracted from 0.75 to 3 mL of plasma using Plasma/Serum Circulating RNA and Exosomal Purification kit (Norgen, cat 42800). The residue of DNA was digested using Baseline-ZERO DNase (Epicentre) and then cleaned up using RNA Clean and Concentrator-5 kit (Zymo). RNA was eluted in 12   elution buffer. cfRNA sequencing libraries were prepared by SMARTer Stranded Total RNAseq - Pico Input Mammalian kit (Clontech) from 6   of eluted cfRNA according to the manufacturer  instructions. Short read sequencing were performed on Illumina NextSeq 500  (2x75 bp) platform to the depth of more than 10 million reads per samples.
SMARTer_Stranded_Total_RNAseq_Pico_Input_Mammalian_kit_4