GSM4633778: progenitors (multilineage organoid); Homo sapiens; RNA-Seq
Mesendoderm progenitor cells were dissociated at differentiation day 5 with Accutase and cell pellet lysed with Trizol (Ambion) and RNA was extracted using the Direct-zol Miniprep kit (ZymoResearch). RNA-seq libraries were created using the SMARTer Stranded Total RNA Sample Prep Kit (Takara Bio). Libraries were sequenced using NextSeq500/550 High Output v2.5 kit (spiked with 10% PhiX; Illumina) to a minimum depth of 29 million reads per sample.