GSM1906817: DS_T2_PEG; Brassica rapa; RNA-Seq
Total RNA from shoot samples was extracted using the RNeasy Mini Kit and treated with DNase. The quantity and quality of all RNA samples were checked using the Fragment AnalyzerTM Automated Capillary Electrophoresis system (Advanced Analytical) before being pooled for cDNA synthesis. cDNA libraries were prepared with the TruSeq Stranded Total RNA with Ribo-ZeroTM Plant kit from Illumina using the manufacturer’s protocol. The libraries were quantified using the KAPA library quantification kit (KAPA Biosystems, Wilmington, MA, USA). The libraries were randomized and mixed into three independent multiplexes from different sampling time-points.Each multiplex was freshly prepared and diluted before sequencing on the MiSeq Illumina sequencing platform with paired-end sequencing of 2 x 150 bp reads.