Brazilian strain genomes of Anaplasma marginale
For library synthesis, the DNA was cleaved with transposase (Nextera DNA sample preparation kit - illumina®, San Diego, CA, USA) and purified with a commercial kit (Qiaquick PCR Purification Kit - Qiagen, Hilden, Germany). After that, samples were indexed and ligated to Illumina specific adapters, which, respectively, allow the post-sequencing sample identification and linkage to the flow cell (Nextera Index kit - illumina®, San Diego, CA, USA). The libraries were amplified by Polymerase Chain Reaction (PCR), purified (AMPure XP - Beckman Coulter Inc, Brea, CA, USA), and quantified by Real-Time PCR (RT-PCR) using SYBR Green and PhiX as control (PhiX control kit v3 - illumina®, San Diego, CA, USA).
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