GSM4633892: Control1_Paminus; Homo sapiens; RNA-Seq
Transcripts remaining after an initial selection for polyadenylated RNA were ribo-depleted, and both poly(A)+ and poly(A)- fractions then sequenced Libraries  were  prepared  from 100ng  of  3 total-RNA  samplesusing  the NEBNEXT  Ultra  II Directional RNA Library Prep kit(New England Biolabs#E7760) according to a protocol provided by the manufacturer. Libraries were barcoded using NEBNext Multiplex Oligos for Illumina Set 4(NEB, #E7730S)