GSM2500252: H3K27ac_ChIPSeq_2; Homo sapiens; ChIP-Seq
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with H3K27ac antibody. Libraries were prepared using the ThruPlex DNA-seq kit from Rubicon Genomics using 20ng of ChIP DNA. Two unique tags were used. Two final pools were made. Each library was QC'ed using Qbit HS dsDNA kit for concentration and Agilent Bioanalyzer DNA HS chip for quality and fragment size. DNA was pooled based on qubit concentration, and qPCR was performed using Kappa Biosystems Illumina LIbrary Quant kit on the pool for nM concentration. DNA was then loaded onto an Illumina HiSeq 2500 50bp rapid run v2 flowcell.