GSM4082089: fis HH; Salmonella enterica subsp. enterica serovar Typhimurium; RNA-Seq
RNA was extracted using a QIAGen RNeasy Mini kit. In addition to the included DNase treatment in the kit, DNA was further eliminated by treatment by turbo DNase (Ambion). RNA was finally re-purified using a QIAGen RNeasy Mini kit. rRNA was depleted using a RiboZero kit (Illumina). RNA libraries were prepared for sequencing using standard Illumina protocols