GSM1543975: THP1_OG86_replicate2; Homo sapiens; RNA-Seq
Total RNA was extracted from vehicle or OG86-treated THP1 AML cells using QIAshredder spin columns and an RNeasy Plus Micro Kit (Qiagen, Manchester, UK). PolyA selection using 15µg total RNA was carried out by performing three rounds of selection using a MicroPoly(A)Purist Kit. Barcoded polyA libraries for pooling and sequencing were prepared using 55ng of the polyA selected RNA with a SOLiD Total RNA-seq Kit. Following quantitation of the libraries by Q-PCR using a SOLiD Library TaqMan Quantitation Kit, emulsion PCR was performed using the SOLiD EZBead System prior to sequencing of single-ended strand-specific 50mers using a SOLiD 5500 System (all from Life Technologies, Paisley, UK).