GSM1543540: Meth_Clone_4T1-T_Round_2; Mus musculus; Bisulfite-Seq
Genomic DNA was purified using the QIAamp DNA Blood Mini Kit (Qiagen) and was fragmented using the Covaris LE220 sonicator according to the manufacturer’s instruction to yield a target fragment size of 300 bp. The fragmented DNA was end repaired, A-tailored and ligated to methylated Illumina adapters. Ligated fragments were then bisulfite converted using the EZ-DNA Methylation-Gold Kit (Zymo). Following PCR enrichment, each sample was sequenced on the Illumina HiSeq sequencer generating 76 nt paired-end (PE) reads.