Illumina MiSeq sequencing; qiita_ptid_4749:10719.K22C
bacterial kinetics in early life (first weeks)
10719.K22C
DNA from total intestinal tissue was isolated with the QIAamp DNA Stool Mini Kit (QIAGEN) as instructed by the manufacturer with additional bead beating steps. For in-depth microbiota profiling, 16S rRNA gene V4 region amplicons from fecal DNA isolates were amplified by the 515F/806R primers and sequenced on an Illumina MiSeq platform using 2x300 bp according to established protocols (1).