GSM2691449: LOUCY_IBET151_Exp2_Rep2; Homo sapiens; RNA-Seq
RNA was extracted using the RNeasy Mini Kit (Qiagen). Libraries were constructed using the Smart-seq2 method (Picelli et al., Full-length RNA-seq from single cells using Smart-seq2. Nat Protocols 2014). cDNA was generated using SuperScript II (Invitrogen) and oligo-dT30VN primers (Biomers.net), pre-amplified using KAPA HiFi HotStart ReadyMix (KAPA Biosystems) and purified using Agencourt AMPure XP (Beckman Coulter). Tagmentation and library preparation was performed using the Nextera XT DNA Library Preparation Kit (Illumina).