Scenedesmus bijugus
Whole genome sequencing (WGS) library was prepared with Illumina-compatible NEXTflex Rapid DNA sequencing kit (BIOO Scientific, Austin, Texas, U.S.A.) at Genotypic Technology Pvt. Ltd., Bangalore, India. Briefly, DNA was sheared using Covaris S2 sonicator (Covaris, Woburn, Massachusetts, USA) to generate approx. fragment size distribution from 300 bp to 500 bp. The fragment size distribution was checked on Agilent TapeStation and subsequently purified using Highprep magnetic beads (Magbio). Purified fragments were end-repaired, adenylated and ligated to Illumina multiplex barcode adaptors as per NEXTFlex Rapid DNA sequencing kit protocol. Adapter-ligated DNA was purified using Highprep beads. Resultant fragments were amplified for 4 cycles of PCR using Illumina-compatible primers provided in the NEXTFlex Rapid DNA sequencing kit. Final PCR product (sequencing library) was purified with Highprep beads, followed by library quality control check. Illumina-compatible sequencing library was quantified by Qubit fluorometer (Thermo Fisher Scientific, MA, USA) and its fragment size distribution was analyzed on Agilent 2200 Tapestation.
Green_Algae_Ext1_WGS