BSL2013.125_2013-08-10_BLE-LTER_KA2_< 3 m surface SW_1 m
Eukaryote-specific PCR primers were used for amplification of the V9 region of 18S rRNA genes with forward primer 1391f and reverse primer EukBr according to Earth Microbiome Project Protocol. The forward primer was linked to Illumina adapter as follows: 5' Illumina adapter - Forward primer pad - 2bp linker - Forward primer, 1391F (1391F: AATGATACGGCGACCACCGAGATCTACAC TATCGCCGTT CG GTACACACCGCCCGTC). The reverse primer additionally included a unique 12 bp barcode as follows: Reverse complement of 3' Illumina adapter - 12 bp Golay barcode - Reverse primer pad - Reverse primer linker - Reverse primer, EukBr (EukBr: CAAGCAGAAGACGGCATACGAGAT XXXXXXXXXXXX AGTCAGTCAG CA TGATCCTTCTGCAGGTTCACCTAC)
BSL2013.125.OSUCGRB43