RNA-Seq of Pyropia haitanensis: Parthenosporphyte formation stage
mRNAs are isolated from total RNA with oligo(dT) method. Then the mRNAs are fragmented under certain conditions. Then first strand cDNA and second strand cDNA are synthesized. cDNA fragments are purified and resolved with EB buffer for end reparation and single nucleotide A (adenine) addition. After that, the cDNA fragments are linked with adapters. Those cDNA fragments with suitable size are selected for the PCR amplification.
PFS2