MACE of Ascochyta rabiei in PDB replicate1
Total RNA was isolated from mycelium of A. rabiei isolate P4 growing for one week in PDB shaking media containing ampicillin and kanamycin (50mg/ml each) in darkness at 20ą2 şC. Two independent replicates were performed.RNA was extracted using TRISure.  Potential residual genomic DNA was removed using RQ1 RNase-Free. RNA was further purified using the RNeasy Plant Mini Kit. The libraries were constructed from ?5 ?g of total RNA using the MACE-Kit (GenXPro GmbH, Frankfurt am Main, Germany) according to the suppliers manual. To avoid any possible contaminant or artifacts, the sequences were mapped against the A. rabiei genome and only those that mapped were retained