GSM4237409: CD4+ Naive rep 1; Homo sapiens; RNA-Seq
Frozen PBMCs were thawed, sorted into effector CD4+CTLs (CD3+ CD4+ CD8- CCR7- CD45RA- SLAMF7+ CD28lo) and naive CD4+ Th cells (CD3+ CD4+ CD8- CCR7+ CD45RA+). Cells were lysed in lysis buffer and total RNA was extracted with the RNeasy Plus Micro Kit (Qiagen). Libraries were prepared according to the mcSCRB-seq V2 protocol adjusted for bulk RNA sequencing. https://www.protocols.io/view/mcscrb-seq-protocol-p9kdr4w