GSM3703297: C23_Empty_dox100_rep3; Homo sapiens; RNA-Seq
Total RNA was isolated using a miRNeasy Mini kit (217004, Qiagen). To reduce melanin carryover, cell lysate in QIAzol reagent was heated at 65°C for 5 minutes before the addition of chloroform and phase separation. Sequencing library was constructed following Illumina TruSeq Standard mRNA Library protocol for 150bp paired-end sequencing