GSM2845453: Bulk Hi-C Library RUES2 CP Rep 2; Homo sapiens; Hi-C
Cells are lysed using 10mM Tris-HCl, pH 8.0, 10mM NaCl, 0.2% Igepal CA-630, 1x Protease Inhibitor in ddH2O Library prep was carried out using a similar protocol to the insitu DNase Hi-C protocols (V. Ramani et al. Nat Protoc. 2016). Samples were barcoded during final library PCR and samples were demultiplexed prior to downstream analysis.