GSM1973416: HCT116 H3K27me3, rep2; Homo sapiens; ChIP-Seq
Cells were crosslinked with 1% formaldehyde for 10 min at RT and excess amount of formaldehyde was captured by 0.125 M Glycine. DNA-protein complexes were immunopurified using antibodies to H3K27me3 (Diagenode, CS-069-100), and Dynabeads Protein G (21004D, Invitrogen) by conventional method with slight modification with biological replicate. The immune-complex was reverse crosslinked and associated DNA was purified with ChIP DNA Clean and Concentrator (Zymo research, D5205). Sequencing library construction was performed using TruSeq ChIP sample prep kit (Illumina, IP-202-1012) by following the manufacturer's instruction with 6-8 ng of ChIPed DNA. To assess the quality of the library, amplified DNA was subjected on DNA 1000 chip (Agilent, 5017-1504).