NNS15 template for inverse toeprinting
The mRNA template library used for inverse toeprinting was reverse transcribed using a specialized Illumina sequencing adapter, which encodes a 18nt stretch complementary to the 3  end of the mRNA. The cDNA obtained from this step served as template for PCR amplification using a specialized sequencing adapter with an 18nt stretch complementary to the 3  end of the cDNA. PCR products were then purified using a PCR purification kit, checked for quality and quantity with BioAnalyzer 2100 using the DNA1000 kit.
NNS15 library