Litoditis marina Pm1, food experiment, treatment E.coli, biological replica 1
Genomic DNA from a single nematode was obtained through a lysis protocol, PCR amplification for the 16S rRNA gene fragment was performed using the forward primer (containing the 5’ Illumina adaptor, forward primer pad and linker and the 515f primer) and the reverse primer (containing the reverse complement of the 3’ Illumina adapter, the reverse primer pad and linker, the 806r primer and a Golay barcode). PCR products were cleaned using the Clone-Well Agarose Gels, measured with Qubit and all 52 samples were equimolarly pooled into a single library, which  was checked on the bioanalyzer.
PM1E1