Single-cell DNA-seq of homo sapiens: adult male spermatozoa: Sample 20180202.NC17a
"Libraries were generated from sperm cells with our method Sperm-seq: we decondensed sperm nuclei using reagents that mimic the molecules with which the egg unpacks the sperm pronucleus. These decondensed sperm nuclei are then encapsulated with barcoded beads in microfluidic droplets in which the sperm genomes are individually barcoded and amplified using modifications to 10X Genomics GemCode technology, which was developed to perform linked-read sequencing from naked DNA. Libraries were sequenced on an Illumina NovaSeq. Each forward sequence read consists of random primer plus captured genome; we use the first 10 bp of this read as the molecular barcode (unique molecular identifier; UMI). The reverse read is the 14 bp cell barcode reporting the bead with which the read was associated. (Cell and molecular barcodes can be converted to read tags using Drop-seq tools software during data processing.) All data is included in these fastqs, including reads coming from cell barcodes associated with ambient DNA rather than confirmed single sperm cells."
20180202.NC17a