GSM1974112: iPS_Rep2_RNA_seq_library; Mus musculus; RNA-Seq
0.9 million cells were lysed with Trizol (Life Technologies 15596-026) and snap frozen. Total RNA was then extracted and purified using the miRvana miRNA Isolation Kit (Ambion AM 1561). RNA integrity was assessed using the Agilent BioAnalyer and samples with RIN scores >9 were carried forward for sequencing. 350 ng total RNA was prepared for sequencing using the Illumina TruSeq Stranded Total RNA Library Prep kit with RiboZero (Illumina RS-122-2202).  Paired-end sequencing was performed on the NextSeq 500 with 75 cycles per end.