GSM1541664: miRi39; Homo sapiens; miRNA-Seq
Small RNAs were isolated from laser capture microdissected tumor tissues using the Just-a-TubeTM RNA Isolation Kit. Adapters were hybridized and ligated to isolated small RNAs. After reverse transcription, a flowcell adapter PCR (with a universal forward primer and fourteen available reverse primers) was performed to amplify and to apply a unique barcode to each sample within a sample-pool. Subsequently, samples were pooled into 4 pools in equimolar volumes of 2nM.