Genotyping-by-sequencing of Laupala NIL1D.2-F2: adult whole body
We extracted whole genome DNA from whole body using Dneasy Blood & Tossue Kit (Qiagen, Valencia, CA). Genotyping by sequencing library was built by the Genomic Diversity Facility at Cornell University following Elshire et al (2011). DNA was digested with PstI restriction enzyme. Samples were multiplexed and sequenced on Illumina HiSeq 2000 with 100 bp single end reads.
1D_GBS_lib