GSM2498563: Balilla_S1_rep2; Oryza sativa; RNA-Seq
RNA was extracted using TRIzol reagent (Invitrogen) according to the manual. RNA yields and quality were measured using Nanodrop 2000 (Thermo Scientific), and further quantified using Qubit2.0 Fluorometer (Invitrogen). RNA integrity was evaluated using Experion RNA Analysis Kits (Bio-rad) and Experion Automated Electrophoresis Station (Bio-rad) Each library was constructed using 3 μg total RNA using the TruSeq RNA Library Preparation Kit v2 (Illumina).