GSM1808910: 2012-07-26_Bud; Danio rerio; RNA-Seq
Total RNA was isolated using the standard TRIzol (Invitrogen) protocol. Genomic DNA was removed by DNase treatment. Two rounds of PolyA+-RNA purification were performed for each sample. Strand-specific libraries for 76-bp paired-end sequencing were prepared according to a modified UTP-method (Parkhomchuk et al. 2009), as detailed by Levin et al. (2010). Libraries were sequenced on the Illumina HiSeq 2000