GSM3703384: HEK_Lib2_Pro_rep1; Homo sapiens; OTHER
Total RNA was isolated using Qiagen RNeasy kit, and mRNA was subsequently isolated using PolyA purist MAG kit. cDNA was then synthesized using Superscript III From input DNA library (before transfections) or cDNA output, short sequences encompassing 10bp unique barcodes were amplified using Q5 high-fidelity polymerase and primers introducing Illumina TruSeq adapter sequences