GSM1641610: HU124.13; Vitis vinifera; RNA-Seq
Leaves from each plant were collected at two time points (1 day and 5 days after inoculation) and immediately frozen in liquid nitrogen. RNA was extracted using a CTAB extraction protocol followed by a cleanup step with the Qiagen RNeasy Plant Mini Kit. High quality RNA samples were obtained from all accessions, time points (1 and 5 days post inoculation), and treatments (healthy and PM infected). cDNA libraries were sequenced in single-end mode with 50 cycles at the DNA Technology core at the UC Davis Genome Center using an Illumina HiSeq2500 sequencer. A total of 8 HiSeq2500 lanes were sequenced with each individual lane loaded with a 12 libraries comprising all replicates (3 biological replicates) of each infection time point (1 and 5 dpi) and treatment (powdery mildew infected and healthy controls) of a specific genotype