captured sequencing of ~100,000 SNPs (meMADSEQ probes) of  fibroblast from normal and affected side of hemihyperlasia patient : Sample S1
We designed the capture probes from Roche NimbleGen: 160407_HG19_MadSeq_EZ_HX1 (Catalog No. 06740260001). Libraries were prepared according to Roche-NimbleGen's instructions. 1ug of DNA was fragmented, end-repaired, dA-tailed, and ligated Illumina's TruSeq adapters. After adapter ligation, DNA was PCR amplified with Illumina primers. Then, the purified libraries were captured with SeqCap probes following the Roche-NimbleGen's instructions. The captured products were further amplified and sequenced on the Illumina HiSeq 2500 system following the manufacturer's protocols.
N0_meMADSEQ