GSM1641312: SW480/pRTR +DOX  miRNA-Seq; Homo sapiens; miRNA-Seq
Total RNA was isolated using the Trizol reagent (Life Technologies) following the manufacturer’s protocol. The small RNA fraction  (representing lengths of about 18 to 25 nt) was gel-purified. In short, an adenylated adapter was ligated to the 3’ end of the small RNA by a truncated T4 RNA Ligase 2, followed by the ligation of the 5’ adapter by T4 RNA Ligase 1 (NEB). After reverse-transcription with a specific primer, the cDNA was amplified by PCR. The correct PCR product was gel-purified, eluted in elution buffer, precipitated and solved in water. The quality of the libraries was assessed by qPCR and Bioanalyzer measurements.