GSM1541791: 38-Per_rep2 [BS-Seq]; Homo sapiens; Bisulfite-Seq
DNA was extracted with MasterPure kits Whole genome bisulfite sequencing libraries were generated using TruSeq DNA Sample preparation protocol (Illumina) with modifications. Bisulfate conversion was done after gel size selection using EZ DNA Methylation-Gold Kit (Zymo Research). To amplify bisulfate converted library Kapa Hifi Uracil+ (Kapa Biosystems) polymerase was used.