GSM2498486: BEZ-235 & SAHA 1; Homo sapiens; RNA-Seq
Total RNA was harvested with the miRNeasy micro kit (Qiagen) with Dnase treatment on-column. Library prep was performed with the TruSeq Stranded mRNA Library Prep Kit LT (Illumina), following manufacturer’s instructions. To assess the quality of the library, BioAnalyzer (Agilent) was used and concentrations were checked by performing qPCR with the Kapa Library Quantification Kit (Kapa Biosystems).