GSM4082884: LPS-serum-injection; Mus musculus; RNA-Seq
Cell suspension mixture of two brains randomly picked from each treatment, was generated using MACS Adult Brain Dissociation Kit (Miltenyi Biotec, USA) following the manufacturer's instruction. Messenger RNA from approximately ∼7,000 cells, captured and lysed within nanoliter-sized gel beads in emulsion, was reverse transcribed and barcoded using polyA primers with unique molecular identifier sequences before being pooled, amplified, and used for library preparation. Cells were then processed through the Chromium Single-cell 3' v2 Library Kit (10× Genomics) by the Oebiotech (Shanghai, China). Briefly, 10,000 cells were loaded onto a single channel of the 10× Chromium Controller.