GSM1542481: sample_F_head_3; Tribolium castaneum; RNA-Seq
Total RNA was extracted using ZR Tissue & Insect RNA MicroPrep™ (Zymo, #R2030) according to the manufacturers protocol. Library preparation for RNA-Seq was performed using the TruSeq RNA Sample Preparation Kit (Illumina, Cat. N°RS-122-2002) starting from 400 ng of total RNA . Accurate quantitation of cDNA libraries was performed by using the QuantiFluor™ dsDNA System (Promega). The size range of final cDNA libraries was determined applying the DNA 1000 chip on the Bioanalyzer 2100 from Agilent (280 bp). cDNA libraries were amplified and sequenced by using the cBot and HiSeq2000 from Illumina (PairedRead; 2x100 bp).