S. beccarii testes 1
Testes pairs were dissected from mature individual males of S. beccarii and immediately transferred to a tube of 10ul of RNAlater (Ambion). 20 pairs of testes were pooled and RNA was extracted using the mirVana RNA Isolation kit (Ambion) according to the manufacturer’s protocol and measured using the NanoDrop ND-8000. Samples were sent to Cofactor Genomics (St. Louis, MO) for Illumina GA sequencing of 60 bp pair-end reads.
SB_s_2