GSM3703673: H3K9|14ac ChipSEQ PBS; Mus musculus; ChIP-Seq
For ChIP-SEQ  ~1 x 10^7 primary myoblasts were cross-linked in 1% formadehyde for 15 min, with constant agitation, and quenched with 150mM glycine for 5 min. Micrococcal nuclease (New England Biolabs, Inc.) was used to fragment chromatin to a range of 200-400 bp. Chromatin was incubated with H3ac antibody (Millipore, 06-599) overnight at 4°C with constant agitation. Protein A Dynabeads was incubated with antibody-protein complexes for 2 hr at 4°C with constant agitation. After de-crosslinking, DNA was isolated using a MinElute column (Qiagen). Cells were trypsinized and then washed in PBS before downstream processing steps Sequencing libraries were constructed according to Illumina protocols.