Arachis hypogaea transcriptional gene finding- Pchu
To isolate genome-wide expression genes from peanut, a high yield and quality peanut cultivar (Arachis hypogaea cv Minhua6) was used for gene discovery. Eight tissues of root, stem, leaf, flower, peg, pod perecarp, testa and embryo of different developing stages and yong seedlings were used for RNA extraction and the equally mixed RNA was used to prepare a whole tissues cDNA library. Additionally, samples from treatments of biotic and abiotic stresses including low temperature and drought, low Ca2+ stresses, hormone treatments (ethylene, abscisic acid, salicylic acid and paclobutracol), and Aspergillus flavus, Rolstonia solanacearum inoculation carried out in peanut, and their corresponding controls were taken for RNA extraction, respectively. Then equally mixed stressed RNAs and mixed control RNAs were used to prepare two cDNA libraries, a stressed cDNA and a control cDNA pools. Then, cDNA libraries were subjected to 454 GS FLX Titanium Pyrosequencing, respectively.
Peanut whole tissue library