MACE of Ascochyta rabiei infecting chickpea 96hai replicate 1
Total RNA was isolated from  A. rabiei infecting chickpea leaves 96 hai. Three independent replicates were performed.RNA was extracted using TRISure.  Potential residual genomic DNA was removed using RQ1 RNase-Free. RNA was further purified using the RNeasy Plant Mini Kit. The libraries were constructed from ?5 ?g of total RNA using the MACE-Kit (GenXPro GmbH, Frankfurt am Main, Germany) according to the suppliers manual. To distinguish A. rabiei reads from those originating from chickpea, reads were mapped against our own preliminary A. rabiei genome assembly and against the chickpea transcriptome reported by Hiremath et al (2001) using the TopHat2.0.3 software. Only reads mapping to the A. rabiei genome but not to the chickpea transcriptome were retained