Tegillarca granosa haemocytes microRNA transcriptome in response to Cd
Two small RNA libraries pooled from control group (C) and heavy metal treatment group (E) were constructed. Total RNA was extracted using Trizol (Invitrogen) according to the manufacturer’s protocol, and the quantity of RNA was examined by using an Agilent 2100 Bioanalyzer. After collecting RNA with small size ranging from 20nt to 30nt, a pair of Illumina proprietary adaptors were ligated to their 5’ and 3’ ends, followed by reverse transcription. The two generated small cDNA libraries were amplified by PCR with primers complementary to the adaptor sequences. Subsequently, the libraries were deep sequenced by Illumina Hiseq2000 according to the manufacturer’s instructions.
Tegillarca granosa haemocytes microRNA