GSM1545834: rhesus_trophoblasts; Macaca mulatta; Bisulfite-Seq
Trophoblast cells were isolated from Rhesus monkey (Macaca mulatta) placental tissue (Gestation Day: 40–65 days) using procedures we have previously described (Douglas and King 1990; Soghomonians et al. 2002; Kumar et al. 2012).  These cells are 95% cytokeratin 7-positive and 5% vimentin-positive, consistent with a predominantly trophoblast population.  DNA was purified using Qiagen's Puregene kit.  MethylC-seq libraries were made using the Epicentre EpiGnome Methyl-Seq kit according to the manufacturer's recommendations except that 14 cycles of amplification were performed.