GSM2344944: Sample 29_IC_saline; Rattus norvegicus; RNA-Seq
The RNA was extracted by homogenizing in RNA STAT-60 (Teltest, Friendsood, TX). RNA was separated from DNA and protein using chloroform, and the total RNA was precipitated with isopropanol. cDNA libraries were created by reverse transcribing the RNA and creating the second strand. Blunt ends were phosphorylated and “a-tailed” so that adapters could be ligated to both ends. Adapters were individually “bar coded” and thus samples were not pooled despite having 4 samples per flow cell, yielding n=7-8 for each condition (total N=30).