GSM1544041: WT_H3K9me2_2; Danio rerio; ChIP-Seq
Sonication was performed using on a Bioruptor instrument (Diagenode) using 7 cycles of 30s “ON”, 30s “OFF” on the “High” setting. ChIP was performed using the Millipore EZ-ChIP kit according to the manufacturer protocol. ChIP DNA was sequenced by Illumina Hi-Seq sequencer with multiplex. An anti-H3K9me2 (AbCam) and an isotype control (AbCam) antibodies were used at 2μg per sample.