Propionibacterium acidipropionici F3E8_illumina
DNA-sequencing and de novo assembly of the different Propionibacterium strains, first DNA was extracted using PureLink Ggenomic DNA Mmini kit (Invitrogen Cat. No. K1820-01) and quantified using Nanodrop 1000 (Thermo Scientific) and Qubit dsDNA BR assay kit (Life Technologies Cat. No. Q32850).  The quality of the DNA was determined by running a 1% agarose gel with DNA gel stain SYBR safe (Life Technologies Cat. No. S33102).  The gel was visualized in using a ChemiDoc MP system (Bio-Rad).  The Illumina platform was used to sequence the genome.  Sequencing was performed using TrueSeq Illumina 300 PE. Libraries were prepared using the Illumina TrueSeq DNA HT sample preparation kit (Illumina Cat. No. FC-121-2003). The assembly of the reads was performed using the SPAdes genome assembly algorithm.
PACF3E8