Immune repertoire-seq of canis familiaris: left popliteal lymph node with TZL - tech rep 2
Genomic DNA was extracted from scraped cytology slides of the patient's lymph node; the lymphocyte antigen receptor loci TRB, TRG and IGH were amplified by multiplex PCR using primers targeting the V/J genes; this is technical replicate 2/2; library preparation was done using the NEB Ultra II kit as per manufacturer's instructions.
16-051749.1-2