GSM3103932: EnMc X LMEB3 replicate 2; Mus musculus; RNA-Seq
Total RNA was extracted from three independent biological replicates from each xenomitochondrial cybrid cell line using a miRNeasy RNA extraction kit (Qiagen, Cat. #217004) with an on-column DNase I (Qiagen) treatment to eliminate contaminating genomic DNA, according to manufacturer's instructions. Transcriptome-wide mRNA sequencing was performed by the Australian Genome Research Facility (Melbourne, Australia). RNA purity and integrity were confirmed by BioAnalyser (Agilent, CA). Libraries were created with TruSeq RNA v2 kit (Illumina), barcoded and sequenced on a HiSeq 2000 sequencer (Illumina), producing 100bp single-end reads, with a depth of 10 million reads per sample.