Gm12878-Illumina
Short-read-sequencing ~100 ng of Poly-A-RNA was fragmented with 10 x fragmentation buffer (Lifetechnologies, #AM8740) for 2 min at 70 °C. cDNA was synthesized using Life Technologies’ Superscript double-stranded cDNA synthesis kit (catalogue #11917-010) and reverse transcription (RT) was primed with random hexamers. The cDNA was end-repaired, A-tailed and Illumina Paired-end adapters were added. After size-selection on an agarose gel and PCR amplification samples were sequenced on the Illumina Hi-Seq 2000. For each line 2 x 101bp paired end reads were generated.