GSM2500308: BAZIA_KO_WT_d11.Control SAM24323247; Homo sapiens; RNA-Seq
HeLa cells (CL132529) were subjected to genome editing using the Cas9 technology to abrogate expression of the chromatin regulator BAZ1A. Lentiviral transduction was then used to re-express either BAZ1A wild-type or the BAZ1A* mutant in BAZ1A knockout cells. All genome edited cell lines have been cloned and passed in-house QC (mycoplasma-free and cell line authenticated).Cells were grown in standard conditions to 50% confluence. Cells were either unchallenged or subjected to UVC irradiation (200 J/m2) to cause DNA damage. One hour after irradiation, total RNA was extracted from 1×106 cells using the RNeasy Mini Kit (QIAGEN) including the on-column DNase digestion.  RNA extractions was performed in triplicates.