GSM1907873: RISC-associated small RNA from NoDice(4-25)ΔPKR expressing N-terminally truncated Dicer N1 transfected with pCD-Rluc; Homo sapiens; RIP-Seq
RISC-bound miRNAs 48 transfection were isolated by immunoprecipitation using a monoclonal antibody that recognizes all human Argonaute proteins (Abcam: AB 57113), TRIzol, Illumina TruSeq kit All small RNA input was processed with the TruSeq Small RNA Sample Preparation Kit (Illumina). Adapter-ligated RNA was reverse transcribed using SuperScript III (Life Technologies) and amplified using GoTaq green PCR master mix (Promega) with TruSeq 3′ indices.  DNA bands corresponding to ~145 bp were isolated by gel electrophoresis on 10% polyacrylamide gels.