GSM1971438: IP12_miR22_Esp2; Mus musculus; RIP-Seq
After cell lysis, two lysate aliquotes were taken to analyze total RNAs and proteins and the remaining was immunoprecipitated with anti-Ago2 antibody using the RNA-binding protein immunoprecipitation Magna RIP Kit (Merck Millipore, USA) following the manufacturer’s instructions. Ago2 bound RNA was extracted using TRIzol reagent (Invitrogen, Thermo Scientific, USA). Amplification of cDNA from Ago-2 co-immunoprecipitated RNA was performed using the Ovation RNA-Seq system V2 (Nugen), then cDNA was fragmented and ligated into a sequencing library using Ovation Ultralow library system (Nugen)