GSM3104195: 0_DAT_Control_4 [degradome]; Triticum aestivum; OTHER
Leaf tissue was collected from all heat-stressed and control individual plants immediately at the end of the stress period, i.e., time point zero day after treatment (0 DAT), and at one and four days after treatment (1 and 4 DAT). Total RNA was isolated from all 432 leaf tissue samples using TRI-reagent (Ambion, Naugatuck, CT) as previously described (Ragupathy et al., 2016; Scientific Reports, 6, 39373). Degradome libraries were constructed from 75 µg of total RNA as described in Zhai et al. (2014) in Methods, 67, 84-90.  Twelve random libraries were pooled in equimolar amounts and two HiSeq2000 single end 50-bp reads were obtained by the McGill University and Genome Quebec Innovation Centre. Degradome (also known as Parallel Ananlysis of RNA Ends) sequencing