GSM4008788: Infected_4.5hpi; Emiliania huxleyi; Coccolithovirus; RNA-Seq
RNA capture: Plates were forzen at -80C and thawed. Lysis solution (ddH2O, 0.1%triton, RNase inhibitors) was evaporated and reverse transcription was performed directly in the wells. MARSseq library prepartion was performed as described in Keren-Shaul et al. 2019 (https://doi.org/10.1038/s41596-019-0164-4). Briefly, cDNA from different cells (barcoded in the reverse transcripiton step) was pooled and amplified by in vitro transcription. Amplified RNA was then fragmented, reverse transcribed, and Illumina adaptors were added by PCR.