GSM3259885: Placenta from AVP-infused pregnant mouse replicate 4; Mus musculus; RNA-Seq
RNA from placentas of pregnant GD12.5 mice were isolated using mirVanaTM RNA isolation kit (ThemoFisher Scientific: AM1560) per manufacturer's instructions. 500 ng of RNA was fragmented, converted to cDNA and ligated to sequencing adaptors containing indexes using the Illumina TruSeq stranded mRNA sample preparation kit (Cat. #RS-122-2101, Illumina, Inc., San Diego, CA).  The molar concentrations of the indexed libraries were measured using the 2100 Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA) and combined equally into pools for sequencing.  The concentration of the pools were measured using the Illumina Library Quantification Kit (KAPA Biosystems, Wilmington, MA)