GSM1908011: ChIP-seq_Mouse_E2F1; Mus musculus; ChIP-Seq
Fixation: 10 min 1% formaldehyde. Nuclear extracts sonication: Bioruptor 14 time 30s on / 30s off and cleared by centrifugation. Precleared: protein A agarose / salmon sperm DNA. Immunoprecipitation: anti–E2F1 c-20 antibody (santa cruz sc-193). Washed: low salt, high salt, LiCl and TE buffer. Elution: NaHCO3 1% SDS buffer. Decrosslinking 65°C overnight. DNA purification: minielute (Qiagen). DNA quantification: Qubit  (Life Technology). Illumina TruSeq ChIP Sample Prep reagents, according to the provider's protocol