GSM1908735: DM_RNA-seq; Mus musculus; RNA-Seq
Chromatins from myoblasts and myotubes were isolated and sonicated to 100-300nt for immunoprecipitation with antibodies Purified RNA was fragmented and the cDNA was synthesized by reverse transcription. The resulting double-stranded DNA fragments were end-repaired and A-nucleotide overhangs were added. ChIP-seq libraries were constructed closely according to the standard illumina pair-end ChIP-seq procedure After the attachment of anchor sequences, fragments were PCR-amplified using Illumina primers.