replicate2
Lungs  were dissected from CD1 mouse embryos collected at E11.5 in cold PBS.  Isolated left lobes were grouped by stage, placed in dispase, and  mechanically dissociated with tungsten needles. After 10 minutes in dispase  at room temperature, DMEM without HEPES and supplemented with 5% fetal bovine  serum (FBS, Atlanta Biologicals) was added, and cell suspensions were passed  through a filter with 40-m-diameter pores. The resultant cell suspensions  were then processed by the Princeton Genomics Core Facility. The two sample  groups were processed separately. Cells were loaded and processed using the  Chromium Single Cell 3 Library and Gel Bead Kit v2 on the Chromium  Controller (10X Genomics) following manufacturer protocols. Individual cells  were encapsulated in droplets with single gel beads carrying unique barcoded  primers and then lysed. Then cDNA fragments were synthesized, barcoded, and  amplified by PCR.
rep2