GSM4008774: LCA3 D4; Homo sapiens; RNA-Seq
Epithelial tissue from the gut MPSs and hepatocytes with Kupffer cells from the liver MPSs, as  well as Treg/Th17 cells were collected and mRNA was extracted using the PureLink RNA mini kit  (Thermo Fisher, cat. no. 12183018A). Total RNA was analyzed and quantified using the Fragment  Analyzer (Advanced Analytical), and Illumina libraries were prepared with Kapa RNA HyperPrep  with RiboErase (Kapa Biosystems, cat. no. 08098107702). After cDNA fragmentation (Covaris  S2), cDNA was end-repaired and adaptor-ligated using the SPRI-works Fragment Library System  I (Beckman Coulter Genomics). Adaptor-ligated cDNA was then indexed during PCR  amplification, and the resulting libraries were quantified using the Fragment Analyzer and qPCR  before being sequenced on the Illumina HiSeq 2000 (Illumina) in High Output mode. 4 lanes 40-50 nt single-end read  with an average depth of 15-20 million or 5 million reads per sample were sequenced.