Cas9 editing of AAVS1 off-target sites
SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage
1736_E9_hg38_chr8:17626798-17626820_HEK293_control_rep_1