GSM1971012: RNA-seq_Control_rep1; Homo sapiens; RNA-Seq
Total RNA was harvested using RNeasy Mini Kit (QIAGEN, Germany). Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. Total RNAs were extracted from H2O2-treated HCT116 and untreated HCT116 cells with RNeasy Mini Kit (QIAGEN, Germany). Quality control was performed using Bioanalyser 2100 (RNA nano kits, Agilent). mRNA-Seq libraries were generated from total RNA with polyA+ selection of mRNA using the TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego, CA), and then subjected to transcriptome sequencing on the Illumina Hiseq 2000