GSM2498437: Col-0 H3K27me3 7DAG rep3; Arabidopsis thaliana; ChIP-Seq
Chromatin was extracted from 7-day old whole seedlings (150 seedlings) as previously described by Reimer & Turck 2010 Methods Mol. Biol. Clifton NJ 631, 139–160 . Anti-H2Aub (Cell signaling technology, 8240S) and anti-H3K27me3 (Diagenode, C15410069) antibodies were used for chromatin immunoprecipitation. All libraries were prepared by the Ovation® Ultralow Library Systems (NuGEN) following manufacturer’s instruction using 80% of a typical ChIP as starting material. After amplification for 16 PCR cycles, DNA of a size range between (200-500bp) was purified from an agarose gel. Amplification was confirmed by testing an aliquot of the library before and after amplification by quantitative PCR.