GSM1808181: SH0012 30h; Escherichia coli; RNA-Seq
The bacterial cells from 5L fermentation were collected at 20h, 30h, 40h and centrifuged for 5min to pellet the cells. The cells then mixed with 500mL of RNA protectant bacterial reagents to stabilize RNA and stored at -80°C until use. Total RNAs were isolated using RNeasy mini kit and were treated with DNase to remove DNA. The RNA-Seq library was constructed with TruSeq RNA Preparation Kit.