GSM4083110: Tet1Control_SC_1; Mus musculus; RNA-Seq
Total RNAs were obtained from oligodendroglial-enriched cell suspensions, isolated from P60 spinal cords tissues, either from control or Tet1 mutant mice, either from unlesioned or lesioned tissues. We used AllPrep RNAeasy Mini Kit (Qiagen), with on-column DNase treatment during the RNA isolation, in accordance with the manufacturer's protocol, to isolate RNA. RNA purity was assessed by measuring the A260/A280 ratio using a NanoDrop, and RNA quality checked using an Agilent 2100 Bioanalyzer (Agilent Technologies). RNA libraries were prepared for sequencing using standard Illumina protocols at the Cornell Genomic core.