GSM3702815: Control replicate 1; Mus musculus; RNA-Seq
RNA was isolated with TRIzol (Invitrogen, CA, USA). RNA purity was checked using the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA) and gel electotrophoresis. The concentration was measured using Qubit ® RNA Assay Kit in Qubit ® 2.0 Flurometer (Life Technologies, CA, USA). Then, RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). RNA libraries were prepared for sequencing using standard Illumina protocols