GSM4572070: Cd_mac_2; Mus musculus; Candida dubliniensis; RNA-Seq
Candida-only cultures were scraped to detach fungal cells and pelleted by centrifugation, followed by resuspension in ice cold water and centrifugation again. For Candida-macrophage co-cultures, medium was aspirated and ice cold water was added to lyse macrophages. Wells were then scraped and samples were pelleted by centrifugation, resuspended again in ice-cold water followed by further centrifugation. Fungal cell walls were digested with zymolyase at 37 °C, followed by RNA extraction using the SV Total RNA Isolation System (Promega). Libraries were constructed using the TruSeq Stranded RNA library preparation protocol. Sequencing was carried out using the HiSeq2000 platform (Illumina) to obtain 15-58 million 2 x 100 paired-end reads. All library preparation and sequencing was performed by Axeq Technologies.