GSM2849298: SYK-KO EGF rep1; Homo sapiens; RNA-Seq
Isogenic OVISE cell lines with SYK knockout was treated with EGF (BD) at 50 ng/mL for 24 h. RNA was isolated and purified using the RNeasy Plus Mini Kit (Qiagen). The RNA quality of the total RNA was assessed using the Agilent 2100 Bioanalyzer RNA Nano Chip; RIN values ranged between 9.4 and 10.0. Paired-end index libraries were constructed using a standard protocol provided by Illumina.