16S rRNA  sequencing of mouse cecal microbiota
Genomic DNA was isolated from mouse cecal sample. The DNA concentrationwas estimated using Qubit Fluorimeter (V.3.0). The V3-V4 region of 16S rRNA was amplified usingspecific V3 Forward primer CCTACGGGNBGCASCAG and V4 Reverse primer GACTACNVGGGTATCTAATCC.The amplified product was checked on 2% agarose gel and gel purification was done to removenon-specific amplifications.5ng of amplified product was used for library preparation using NEBNext UltraDNA library preparation kit. The library quantification and quality estimation were done in Agilent 2200TapeStation. The prepared library was sequenced in Illumina HiSeq 2500 with 2*250 cycles chemistry
Vanco_balbc_2_day4