GSM2850010: 12+6h BPDL_1; Chlamydia trachomatis; RNA-Seq
RNA was collected after 0h treatment (samples A-E), 3h treatment (samples F-N), or 6h treatment (samples O-T) in Trizol and processed according to the Ribopure bacteria kit (Ambion). DNased RNA was applied to the MegaClear column (Ambion) to remove small RNAs, followed by two rounds of eukaryotic mRNA and rRNA depletion with the MicrobEnrich kit (Ambion), and 1-2 rounds of prokaryotic rRNA depletion with MicrobExpresss kit (Ambion). RNA was ethanol precipitated, and tested for integrity by RNA fragment analysis. RNA was fragmented using RNAse III and cDNA was prepared as per the Ion Total RNA-seq Kit V2, and sequenced on an Ion Proton chip