GSM4083997: hESC_Dox5 scRNA-Seq; Homo sapiens; RNA-Seq
Non-adherent progenitors at day 22 of differentiation (untreated and 24-hour Dox treatment) were sorted for CD45+ CD34+ and RUNX1C+. Cells were re-suspended in 80 μl at a concentration of 100-1200 cells/μl for evaluation of cell viability prior to loading of 4000 single cells on a Chromium Single Cell Instrument (10X Genomics). Library generation for scRNA-seq was performed by the Genomics Birmingham Sequencing Facility using the Chromium Single Cell 3′ Library and Gel Bead Kit v2 (10X Genomics). Libraries were paired-end sequenced on an Illumina NextSeq machine using the cycle parameters recommended by 10X Genomics.