GSM1423184: WT_24h_1; Mus musculus; RNA-Seq
Trizol extraction of total RNA was performed according to the manufacturer's instructions. poly-A pull-down were used to enrich mRNAs from total RNA samples (200ng-1ug per sample, RIN>8 required). For library preparation, the Illumina TruSeq RNA prep kit was used. Libraries were sequenced on the Illumina HiSeq2000 at the Columbia University Genome Center. Samples were multiplexed in each lane, which yields the targeted number of single-end/paired-end 100bp reads for each sample, as a fraction of 180 million reads for the whole lane.