deep seawater SSU rRNA (16S) gene survey
We collected deep subseafloor crustal fluids from borehole observatories         located on the Juan de Fuca Ridge flank. Fluids were filtered to collect the microbial         size-fraction and preserved at -80 C until transport to the laboratory. DNA was extracted         from the samples and the SSU rRNA genes were amplified from the environmental genomic DNA         using a barcoded primer (515F/806R) approach to perform sequencing of multiplexed samples         (Caporaso et al., 2011; PNAS).
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