GSM1782948: wt L C T0 R1; Zea mays; ncRNA-Seq
Leaf and shoot apical meristematic area samples of four-five plants for each combination of genotype-treatment-sampling time point were pooled together, flash-frozen in liquid nitrogen and stored at -80°C. Total RNA was extracted from frozen tissue using the Spectrum Plant Total RNA Kit (SIGMA), using "Protocol A" with 750µL of Binding Solution, to recover more of the small-sized RNA, and subjected to On-Column DNase Digestion (SIGMA). The sRNA libraries were produced using the TruSeQ® small RNA Sample Preparation Kit (Illumina) and sequenced on an Illumina Hiseq2000 platform by the Istituto di Genomica Applicata (Udine, Italy).