Illumina HiSeq 2500 paired end sequencing
gDNA-Seq libraries to generate 125 bp read pairs were constructed
Nextera XT
One nanogram of DNA per sample was used for the construction of the libraries. First, the DNA is simultaneously fragmented and tagged with adaptater sequences by a transposome (“tagmentation step). Then, the tagmented DNA is enriched with 12 cycles of PCR using indexed PCR primers. After clean-up using Ampure XP beads (Beckman Coulter Genomics, ref.A63881), the final libraries were validated with a DNA High Sensitivity Labchip on a Bioanalyzer (Agilent) and quantified with microfluorimetry (Qubit).