GSM1782565: IFE_normal_single-end; Mus musculus; OTHER
Tumours were dissected, minced, digested and filtered to get single cell suspension. Specific cell populations were isolated by FACS using PE-conjugated anti-CD45, PE-conjugated anti CD31, PE-conjugated anti-CD140a and APC-Cy7-conjugated anti-Epcam. Sorted cells were collected into lysis buffer for RNA extraction and ATAC-Seq. Illumina Nextera DNA Library Prep Kit