Illumina HiSeq 2500 paired end sequencing; RNA-Seq of liver tissue samples from northern pike (Esox lucius), coho salmon (Oncorhynchus kisutch) and Arctic charr (Salvelinus alpinus)
RNA-Seq of liver tissue samples from northern pike (Esox lucius), coho salmon (Oncorhynchus kisutch) and Arctic charr (Salvelinus alpinus)
Okis_1_Liver_RNA_p
Samples were sacrificed according to protocols at each of the facilities from where samples were obtained. For Charr and northern pike samples, fresh liver tissues were immediately removed and stored in RNAlater (Invitrogen). Samples were stored during shipping at ambient temperature (3-10 days) then frozen at -80C long term prior to extraction. For coho, samples were immediately flash frozen, shipped on dry ice and stored longterm at -80C. The liver tissues were disrupted by mixer-mill (Retsch) with homogenization by 3mm tungsten carbide beads (Qiagen) in Trizol (Invitrogen), followed by column clean-up using the RNeasy kit (Qiagen) with on-column DNAseI digestion (Qiagen). Quality control was performed on samples by Experion RNA StdSns assay (BioRad) with RQI >= 8, and quantified by Qubit RNA BR (ThermoFisher). Illumina TruSeq Stranded mRNA libraries were built by the McGill University and Genome Quebec Innovation Centre (Montreal, Canada) following manufacturer recommended protocol.