bacteria:soil
The soil prokaryotic community composition was assessed by Illumina MiSeq sequencing of combinatorial sequence-tagged PCR products using the universal barcoded (Parameswaran et al. 2007) primers 515F and 926R targeting the 16S rRNA gene region (Parada et al. 2016). Sample amplification was performed in triplicate in an Eppendorf Mastercycler PCR machine (Eppendorf, Hamburg, Germany). After pooling the replicate PCR products, the concentration was measured with the TapeStation 2200 using D1000 ScreenTapes (Agilent Technologies, Santa Clara, USA) and samples combined in equal proportions. The final library was then purified and concentrated using the NucleoSpin Extract II kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany). The paired-end DNA library was prepared as described previously (Herbold et al., 2015) and sequenced on an Illumina MiSeq system (Illumina Inc., San Diego, USA) at Microsynth AG (Balgach, Switzerland).
CH9