GSM1974454: global riboseq replicate 1; Saccharomyces cerevisiae; OTHER
Clarified lysate was digested with RNase I and separated on a sucrose gradient.  Monosome fractions were collected and the RNA was extracted and size selected for ribosome footprints. Collected cells were resuspended in lysis buffer and lysed by vortexing with glass beads. RNAs were ligated to a preadenylated DNA linker and reverse transcribed.  cDNAs were gel purified, circularized, and PCR amplified.