GSM4083328: smEWT1: Small RNA EVs wild-type Biorep #1; Homo sapiens; ncRNA-Seq
Total RNA from wild-type, ATG7-/- and ATG12-/- HEK293T EVs and cells was isolated using the Macherey-Nagel NucleoSpin kit (# 740971) according to the manufacturer's protocol. Small RNA libraries were prepared using the SMARTer smRNA-Seq Kit for Illumina (Takara #635030), libraries were amplified for 12 cycles and subject to size selection using SPRI AMPure Beads (Beckman Coulter, #A63880). RNA-seq: Single-end 50 bp read length on a HiSeq4000