Pyrosequencing of 16S rRNA gene amplicon obtained from the seawater sample used for HTC
A surface seawater sample (2 L) from the East Sea was filtered using 0.2-um pore size membrane filter. Total DNA was extracted from the filter and used for amplification of 16S rRNA gene (V1-V3 region; 27f and 519r primers). Amplified DNA was sequenced using 454 GS FLX Titanium platform.
ESS_HTC_Seawater