GSM2690472: SH duodenum mirna; Ovis aries; miRNA-Seq
The total RNA was extracted using Trizol reagent and the RNA of different fragment size was separated using PAGE. Illumina TruSeq samll RNA Sample Prep Kit (Cat#RS-200-0012) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols