GSM4083235: 34_168KOd04; Homo sapiens; RNA-Seq
Total RNA was isolated using TRIZOL reagent, followed by DNase digestion and ethanol precipitation of RNA. ERCC Spike-in was added normalizing to cell number. Total RNA H9 WT and TSC2-/- NCC sample libraries were prepared with ribosome depletion (Illumina # 20020598), all other sample libraries were prepared using the TruSeq Stranded mRNA kit (Illumina #RS-122-2101).