GSM4010441: 15_tumor; Canis lupus familiaris; RNA-Seq
RNA was extracted using the optimised Covaris-FFPE RNA isolation procedure described in: Amini P, Ettlin J, Opitz L, Clementi E, Malbon A, and Markkanen, E (2017) An optimised protocol for isolation of RNA from small sections of laser-capture microdissected FFPE tissue amenable for next-generation sequencing. BMC Mol Biol 18:22. doi: 10.1186/s12867-017-0099-7 SMARTer Stranded Total RNA-seq Kit—Pico Input Mammalian from Clontech/ Takara Bio USA, using 4 ng total RNA per sample; details described in: Amini P, Ettlin J, Opitz L, Clementi E, Malbon A, and Markkanen, E (2017) An optimised protocol for isolation of RNA from small sections of laser-capture microdissected FFPE tissue amenable for next-generation sequencing. BMC Mol Biol 18:22. doi: 10.1186/s12867-017-0099-7