GSM1316731: Rosa chinensis 'Pallida'  RNA-seq; Rosa chinensis; RNA-Seq
Total RNAs were extracted from Flower bud, open flower and senescent flower with CTAB reagent method (Invitrogen). Equal volumes of RNA from flower buds, open flowers and senescent flowers were pooled. RNA quality and quantity were verified using a NanoDrop 1000 spectrophotometer and an Agilent 2100 Bioanalyzer prior to further processing. Total RNA was treated with DNase I prior to library construction, and poly-(A) mRNA was purified with Magnetic Oligo (dT) Beads. Double-stranded cDNA was further subjected to end-repair using T4 DNA polymerase, the Klenow fragment, and T4 polynucleotide kinase followed by a single A dNTP base addition using Klenow 3’ to 5’ exo-polymerase, then ligated with an adapter or index adapter using T4 DNA ligase.