GSM2587542: MEF GTMS RNA-seq_rep2; Mus musculus; RNA-Seq
RNA-seq: RNA extraction was done using the RNesay kit (Qiagen) according to the manufacturer’s instructions. Six RNA libraries were prepared: MEFs, and MEFS at three days of reprogramming by OSKM, GETM, GTMS or GETMS. ESC and TSC samples were re-analyzed from GSE64684. RNA-seq: Library preparation was done with QuantSeq 3′ mRNA-Seq Library Prep Kit by Lexogen. Libraries were subjected to single-end sequencing using Illumina Next-Seq 500 platform.