GSM1543764: BMDM miRNA seq WT1; Mus musculus; miRNA-Seq
Total RNA and small RNAs were isolated as detailed in the manufacturer’s protocols (Clonetech NucleoSpin miRNA) and RNA quality was checked by an Agilent 2100 bioanalyzer (Agilent technologies) RNA libraries were prepared for sequencing using standard Illumina protocols, PAR-CLIP sample libraries were prepared with PAR-CLIP + TruSeq Small RNA prep.