GSM4010826: Validation_miseq_induced_9; synthetic construct; OTHER
Model-derived artificial promoters ordered from Twist Bioscience as an oligo pool; to join two Twist oligos to form each designed sequence, subpools were selectively amplified with designed primers, and sequences were specifically joined using complementary Golden Gate assembly sites. After FACS-sorting and overnight regrowth in the same media used for growth, sorted fractions were miniprepped. PCR was used to verify that at least 10 plasmids were extracted per sorted cell; minipreps were PCR-amplified, further amplified with the addition of bin-specific barcodes, pooled, and amplified briefly again.