GSM3703227: 5125 WT, Nicotine (F); Mus musculus; RNA-Seq
Offspring lungs were collected at PND2, snap frozen in liquid nitrogen and stored at -80˚C. RNA was isolated and purified using Macherey-Nagel purification kit (Germany). Six offspring lung were used for the saccharin group and five offspring lung were used for nicotine group. cDNA libraries were constructed by the Genomic platform of the University of Geneva using the Illumina TruSeq RNA Sample Preparation Kit (CA, USA) according to the manufacturer's protocol. RNA libraries were prepared for sequencing using standard Illumina protocols