AB SOLiD 3 Plus System sequencing
Quantify the miRNA expression
GCMIR3
Total small RNA was obtained from tissue samples using mirVana Isolation Kit (Ambion Inc., US). Concentration and quality were determined using a Nanodrop 1000 spectrophotometer, and purification and size selection were performed using 6% polyacrylamide gel electrophoresis. Using SOLiD small RNA Expression Kit (Ambion Inc., US), 200 ng of small RNA of 150-200 bp were used as template to obtain the miRNA library