GSM2501180: K27M pHGG 1; Homo sapiens; RNA-Seq
Total RNA was extracted from human tumors using the AllPrepDNA/RNA/miRNA Universal Kit (Qiagen) according to instructions from the manufacturer. Library preparation was performed with ribosomal RNA (rRNA) depletion methods according to instruction from the manufacturer (Epicentre) to achieve greater coverage of mRNA and other long non-coding transcripts. Paired-end sequencing was performed on the Illumina HiSeq 2000 platform.