Illumina HiSeq 2000 sequencing; Comparative transcriptomics of Rhizobium mesoamericanum STM3625, Cupriavidus taiwanensis LMG19424 and Burkholderia phymatum STM815 when induced by root exudates of Mimosa pudica
Comparative transcriptomics of Rhizobium mesoamericanum STM3625, Cupriavidus taiwanensis LMG19424 and Burkholderia phymatum STM815 when induced by root exudates of Mimosa pudica
BP-Induced3
Bacteria were grown in VSG medium (Vincent-Succinate-Glutamate, Vincent, 1970) supplemented with root exudates of Mimosa pudica (induced condition) or not (control condition) Total RNA were extracted at mid log pahse of growth (4,5 for BP and CT, 6h for RM) using the RiboPure kit (Ambion), and rRNA were subtracted by two steps of MicrobeExpress kit (Ambion) extracted following  Libraries were built by GATC company using the Trueseq Illumina kit