GSM2350348: Blood BDCA-1+DCs Rep3; Homo sapiens; RNA-Seq
RNA was extracted and column-purified using the Arcturus PicoPure RNA Isolation kit (Applied Biosystems). Genomic DNA was removed by on-column digest with DNase I (Qiagen), according to the PicoPure RNA Isolation kit manual RNA libraries were prepared using the SMARTer Ultra Low Input RNA for Sequencing kit (Clontech Laboratories) followed with Nextera XT DNA Library Prep kit (Illumina).  Libraries were sequenced by 75-bp single-end reading on a NextSeq 500 sequencer (Illumina)