msh2_1_P30
Illumina sequencing library was constructed by sonicating 1.5 *g of DNA until fragment sizes < 2,000 bp were obtained using a bioruptor (Diagenode). Fragments were end-repaired A-tailed and Illumina adaptors were subsequently ligated to the fragment ends.  Samples were run on a 2% low melting temperature agarose gel and DNA in the 175-225 bp range excised and eluted. Fragments were enriched by PCR amplification and final sequencing libraries were generated at a concentration of 10nM.
msh2_1_P30