GSM4009017: Pat9_WM_2_6; Homo sapiens; RNA-Seq
Fresh tissue was dissected by removing the meninges. Single-cell suspesions were generated with a dounce potter followed by passing through a cell strainer. Myelin was removed using percoll gradient centrifugation. As described in mCEL-Seq2 protocol (Hashimshony et al. 2016 and Herman et al. 2018) Adapted from TruSeq Small RNA Library Preparation Protocol