GSM2233079: N2B27_6d_TKO_228; Mus musculus; RNA-Seq
RNeasy Mini Kit (Qiagen) NGS lbrary prep was performed using Illumina's TruSeq Stranded mRNA HT Sample Prep Kit with dual-indexing following Illumina’s standard protocol (Part # 15031047 Rev. D). Libraries were profiled with a DNA 1000 chip on an Agilent 2100 Bioanalyzer and quantified using the Qubit dsDNA HS Assay Kit on a Qubit 2.0 Fluorometer (Life Technologies). All 36 samples were pooled in equimolar ratio and sequenced on 8 HiSeq 2000 lanes for 35 cycles plus additional 16 cycles for the i7 and i5 index reads.