GSM2845422: TamCre DN3-2; Mus musculus; RNA-Seq
Total RNA was extracted from 100K-400K DN3 cells using the RNeasy Plus Micro kit (Qiagen) according to the manufacturer's protocol. DeltaTamCre and TamCre sequencing libraries were prepped from 100 ng of total RNA using the Illumina TruSeq mRNA Sample Prep v2 kit (RS-122-2001, RS-122-2002) accrording to manufacturer's protocol by the University of Michigan Sequencing Core. DeltaMxCre, MxCre, Anti-NRR antibody, and Control IgG antibody sequencing libraries were prepped from 50 ng of total RNA using the Clontech SMARTer Ultra low input RNA kit (6348493) accrording to manufacturer's protocol by the University of Michigan Sequencing Core.