Metagenomic sequencing of seawater DNA: Sample BP_381109-3
"Each DNA library was prepared for sequencing from 50-100 ng of DNA using the Ion Xpress Plus Fragment Library Kit (Life Technologies) with the Ion Xpress Barcode Adapters 1-16 (Life Technologies), using the Ion Shear Plus Reagents and a Pippin Prep instrument (SAGE Science, Beverly, MA) for size-selection. Barcoded libraries were pooled in an equimolar ratio three by three. A total of 3.50 x 107 molecules were used in an emulsion PCR using the Ion OneTouch 200 Template Kit (Life Technologies) and the OneTouch instrument (Life Technologies). The sequencing of the pooled libraries was performed using the Personal Genome Machine (PGM) system with the Ion Sequencing 200 kit and 316 chips (Life Technologies). "
BP_381109-3