GSM4634819: mESCs_WT; Mus musculus; RIP-Seq
Total RNA was harvested by phenol chloroform method. Total RNA was randomly fragmented to size around 200 nucleotides at an appropriate temperature and then subjected to immunoprecipitation (IP) with anti-m6A antibody (Sigma-Aldrich, cat. no ABE572), Dynabeads Protein A (Invitrogen™, cat. no 10002D) and Dynabeads Protein G (Invitrogen™, cat. no 10004D). The immunoprecipitation RNAs were recovered and library was synthesized by SMART principle into the first strand cDNA, amplified and enriched by PCR, and purified by DNA from magnetic bead library fragments to obtain ultrafine RNA methylation of m6A. m6A MeRIP-Seq