metatranscriptome_AnnaTempExp_2012-RNAseq-10degC-day78
RNA was extracted from 10ml pelleted culture, treated with DNase, and rRNA was depleted from purified RNA (3-5 μg) using the Ribo-Zero rRNA removal kit (Bacteria, Epicentre, Madison, WI, USA). A cDNA library was prepared with the Ion Total RNA-Seq Kit v2 (Life Technologies) following the protocol for whole transcriptome library preparation. The library were sequenced with a Personal Genome Machine (PGM), with chemistry for 200 bp libraries.
2012-RNA-10-1