Illumina HiSeq 2000 paired end sequencing; Human Developmental Biology Resource (HDBR) expression resource-RNAseq
Human Developmental Biology Resource (HDBR) expression resource-RNAseq
HDBR584
Human embryonic and fetal tissues were provided by the Human Developmental Biology Resource (www.HDBR.org).  HDBR is a UK tissue bank which is regulated by the UK Human Tissue Authority (HTA; www.hta.gov.uk) and operates in accordance with the relevant HTA Codes of Practice. Tissue samples are collected with appropriate maternal written consent and approval from research ethics committees. Embryonic samples were staged following the staging guidelines outlined in Bullen, P and Willson, D (1997). The Carnegie staging system of human embryos: a practical guide in Molecular Genetics of Early Human Development. T. Strachan, S. Lindsay and D.I. Willson. Fetal samples were staged using foot and knee-heel lengths (Hern WM [1984] Correlation of fetal age and measurements between 10 and 26 weeks of gestations. Obstet. Gynecol.63: 26-32) All nucleic acid preparation was performed by AROS following standardised procedures (AROS – arosab.com). Applied Biotechnology (www.arosab.com). RNA was extracted on the QIAsymphony SP using manufacturer's protocol miRNA v05. Library preparation followed Illumina's guidelines for the TruSeq stranded mRNA stranded LT sample prep kit. (http://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf)