GSM3178723: KOPTK1_dox_3; Homo sapiens; RNA-Seq
Purified RNA was reverse-transcribed with the Superscipt III RT kit (Invitrogen). Quantitative real-time PCR was performed with the Applied Biosystems ViiATM 7 using gene-specific primers and SYBR Green PCR Master Mix (Roche). mRNA was extracted using the RNeasy Mini Kit (Qiagen).