GSM1138175: Rep2 6h; Caenorhabditis elegans; RNA-Seq
Worms were harvested at each sampling time point during the next 8 hours, washed three times with M9 medium, resuspended in TRIzol reagent (Life Technologies) and frozen in liquid nitrogen. The worms were broken open by five repeats of freeze and thaw using liquid nitrogen and a 42 C heating block, before RNA was extracted and purified according to the supplier's protocol with the modification that RNA was incubated with 50% 2-propanol at -80 C overnight for efficient precipitation of small RNA. Small RNA libraries were prepared from extracted total RNA using TruSeq Small RNA Sample Prep Kit (Illumina) according to the supplier's protocol.