GSM2233352: RNA shKdm5ABC Day 0 exp.2; Mus musculus; RNA-Seq
Total RNA from 3T3-L1 cells was purified using TRIzol (Invitrogen) according to the manufacturer's instructions. RNA was treated and the integrity of the RNA was confirmed using a Bioanalyzer 2100 (Agilent Technologies).  4 ug of total RNA was polyA-selected, and subsequently subjected to fragmentation and cDNA-synthesis using the TruSeq RNA Sample Prep kit v2 (Illumina). cDNA-seq libraries were constructed according to the manufacturer's instructions (Illumina) as described in (Nielsen R, Mandrup S, Methods in Enzymology 2014, Vol. 537, pp. 261-279).