Root Sample 1
At 2 weeks of growth 600 mM NaCl solution (treatment) or water (control) was applied to the soil, daily, for 7 days. Three biological replicates for each treatment and species were harvested at 3 hours, 8 hours, 24 hours, and 7 days. Total RNA was extracted from the roots and leaves using the Qiagen protocol and treated with DNase. For each tissue, total RNA from each time point was pooled and underwent Illumina HiSeq 2500 sequencing.