GSM1545317: Brwd1_replicate1; Mus musculus; ChIP-Seq
The DNA for ChIP-Seq libraries was purified by Qiagen PCR Purification Kit and the ATAC-Seq libraries were purified using a Qiagen PCR cleanup kit. For ChIP-Seq DNA libraries were prepared from the sheared chromatin (200–600 bp) by University of Chicago sore facility at Institute for Genomics and Systems Biology, Department of Human Genetics. For ATAC-Seq, following purification of transposed DNA, we amplified library fragments using Nextera PCR Primers (Illumina Nextera Index kit) and NEBnext PCR master mix (New England lab, 0541) for a total of 10-12 cycles. The libraries were then purified using a Qiagen PCR cleanup kit.