GSM1810474: R 0h R2; Arabidopsis thaliana; RNA-Seq
Total RNA was isolated using the EURx GeneMATRIX Universal RNA Purification kit with on-column Dnase digest according to the manufacturer's instructions. Libraries were prepared using the Illumina TruSeqTM sample prep kit v2, Box A, following the protocol according to the guide version September 2012, starting from 4 µg total RNA. The PCR enrichment was done in 34 µL using only half the template available, and followed by an agarose gel purification step and extraction from the gel using the Qiagen MinElute gel extraction kit. Adapters used for multiplexing were 012 and 006 for duplexing, and 012, 006,  019, and 005 for quadruplexing