GSM1543484: Clone 4T1-J_B; Mus musculus; RNA-Seq
RNA was purified and DNase treated using the Qiagen RNeasy Mini Kit The NuGEN Ovation RNA-Seq V2 protocol was carried out on 100 ng of total RNA. cDNA was fragmented using the Covaris LE220 sonicator according to the manufacturer’s instruction to yield a target fragment size of 200 bp. The fragmented cDNA was subsequently processed using the NuGEN Ovation Ultralow DR Multiplex System. Libraries were analyzed on an Illumina Hiseq-2.0 using 76bp reads.