Cas9 editing of AAVS1 off-target sites
SITE-Seq target sites (i.e., biochemical off-targets) recovered with an sgRNP directed to the AAVS1 locus were directly targeted with sgRNPs to investigate whether these sites were accessible to Cas9. These data are shown in Fig 4 in the corresponding manuscript Mapping the genomic landscape of CRISPR Cas9 cleavage
1735_C10_hg38_chr21:43981667-43981689_HEK293_control_rep_1