GSM1812099: MGH36_P1_B04; Homo sapiens; RNA-Seq
RNA from single cells was first purified with Agencourt  RNAClean XP beads (Beckman Coulter) Libraries from isolated single cells were generated based on the Smart-seq2 protocol (Picelli  2014) with the following modifications.oligo-dT were used to prime reverse transcription with  Maxima reverse transcriptase and locked TSO oligonucleotide, which was followed by 20 cycle  PCR amplification using KAPA HiFi HotStart ReadyMix (KAPA Biosystems) with subsequent  Agencourt AMPure XP bead purification as described. Libraries were tagmented using the  Nextera XT Library Prep kit (Illumina) with custom barcode adapters (sequences available upon  request). Libraries from 384 cells with unique barcodes were combined and sequenced using a  NextSeq 500 sequencer (Illumina).