GSM1908008: PS1M_HYY_banana1_TGACCA_L001_R1_001; Musa acuminata AAA Group; miRNA-Seq
Total RNA was extracted and purified from plant samples using RNApure Plant Kit. Then, total RNA (amount >20 μg; concentration≥200 ng/μl) was sent to Illumina Sequencing Platform of Yikong Genomics for sequencing. Total RNA was size-fractionated and isolated to obtain 10-30 nt sRNAs. Subsequently, 5' and 3' primers were ligated to sRNAs to synthesize double-stranded nucleic acids by RT-PCR. PCR products were sequenced by sequencing-by-synthesis (SBS) technique.