GSM2345419: SMAD3_pQ_01_ChIPSeq; Mus musculus; ChIP-Seq
Striatal tissue was homogenized in glass dounce and fixed with formaldehyde for 10 min at room temperature.  Chromatin was fragmented using MNase digestion followed by sonication. SMAD3- or RNA Pol2-DNA complexes were isolated using antibody. Modified ChIP-seq (see PMID 22025700)