GSM4010457: Placebo-arm, Good outcome, Baseline [A_0_T0_DT0817]; Homo sapiens; RNA-Seq
Swabs from the tarsal conjunctiva were collected at baseline and at one, six and twelve months post-surgery. A sterile polyester swab (Puritan) was passed across the conjunctival surface four times, with a quarter-turn on each pass. Swabs were stored in RNAlater (Thermo Fisher Scientific) at 4°C overnight and then long-term at -80°C. RNA and DNA were extracted from swabs using Norgen RNA/DNA purification kit (Norgen Biotek) following manufacturer's instructions. Library preparation was performed using QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen GmbH, Austria), following the manufacturer's instructions. QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina