GSM1908218: ChIP-seq H3K4Me3 D Hhex F; Mus musculus; ChIP-Seq
DNA was fragmented in a Covaris Sonicator (30 minutes, 4°C) then protein-DNA complexes immunoprecipitated from clarified chromatin fractions using H3K27Me3 (2μg; Millipore: 07-449) or H3K4Me3 (2μg; Millipore: 07-473) antibodies and 30μl of Protein A-sepharose. For Hhex-Flag ChIP, 30μl of anti-Flag (M2)-agarose beads (Sigma) was added directly to clarified chromatin fractions. Following reversal of crosslinks and RNaseI and Proteinase K treatments, extracted DNA was purified using the QIAquick purification kit. ChIP-Seq DNA libraries were constructed using TruSeq Nano DNA sample preparation according to the manufacturer’s instructions.