RNA-Deep Seq of ZIKV-PRVABC59: viral RNA extracted from inoculum stock passsaged 3x on Vero cells,  2x on C6/36
Viral RNA was isolated from 1 ml of cell culture supernatant and ~1x10^6 viral RNA templates were converted into cDNA with random hexamers as primers. The cDNA was then split into two multi-plex PCR reactions using the 35 PCR primers described in Quick et. al ({Quick, J et al., 2017.,}). PCR products were then tagged with the Illumina TruSeq ChIP kit and sequenced with a 2 x 300 kit on an Illumina MiSeq.
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