RNA-Seq of Botryococcus braunii: Bb768_10-28
Cells were harvested from Bb768 Race L grown under continuous light 50 ¦Ìmol photons m-2 s -1 173 and 2% CO2 bubling for two days. Total RNA was extracted using Trizol reagent 174 (Life Technology) according to manufacturer's instructions, Rnase-free DNaseI (Life 175 Technology) treated and finally column cleaned (RNeasy mini kit, Qiagen). cDNA library 176 creation for Hi-Seq illumina sequencing PE90 was performed in douplicate or triplicate by AIT 177 Biotech (Singapore).
Bb768_10-28