GSM1542236: CM_NtermGFP_hr02; Dictyostelium discoideum; ChIP-Seq
ChIP-seq: After cellular fixation, we isolated nuclei and prepared nuclear extract by sonication. We used precleared nulcear extract for ChIP experiment with anti-GFP antibodies (Roche, clones 7.1 and 13.1 mixture). After stringent wash, we performed reverse-crosslinking treatment and purified the ChIP DNA. We prepared Illumina multiplexed libraries. We prepared 15 (or 20) individual libraries separately (one from each sample) and added a unique barcode to each library at the final step of PCR amplification. We pooled equal amounts of DNA from each library and sequenced one pool per lane of a flow cell on Illumina Genome Analyzer II using the manufacturer's recommended pipeline (versions 1.2 and 1.3, read length = 50 bases)