WGS of Colestrella sp. MACC-549
Nextera Mate Pair Sample Preparation Kit (Illumina, U.S.A) was used to generate mate- paired libraries according to the manufacturer's protocol for gel-plus version with slight modifications. 13 l of Mate-Paired Tagment Enzyme was used to produce a robust smear within the 7-11 kbp region. The 7-11 kbp DNA fraction was excised from the gel using the Zymoclean Large Fragment DNA Recovery kit (Zymo Research) and the circularized DNA was sheared using Covaris S2. All quality measurements were performed on a TapeStation 2200 instrument (Agilent, U.S.A). Final libraries were quantified using Qubit (ThermoFisher) and sequenced on an Illumina MiSeq instrument using MiSeq Reagent Kit v2 (500 cycles) sequencing chemistry.
APETH