"DNA-Seq of Agave tequila: Single-end, PCR-free"
"Nuclear DNA for library preparations was extracted from young leaf as described (Carrier et al. 2011. Am J Bot 98: e13-15) with minor modifications. The TruSeq? DNA PCR-Free LT Sample Preparation Kit (Illumina, CA, USA) was used to make the library with an average insert size of 590 bp. The library was sequenced on a Miseq instrument (Illumina, CA, USA) using MiSeq Reagent Kit v3 (600-cycle). The reads were trimmed and filtered using Trimmomatic (Bolger et al. 2014. Bioinformatics. doi: 10.1093/bioinformatics/btu1170) on the following criteria: (1) a Q20 quality cutoff; and (2) minimum read length of 100 bp."
Agte_DNA_SE_PCRfree