GSM4634815: Brain_KO1; Mus musculus; RNA-Seq
Brains were removed and RNA was harvested using Trizol reagent. Oligo(dT)-attached magnetic beads were used to purified mRNA. Purified mRNA was fragmented into small pieces and then cDNA was generated using random hexamer-primed reverse transcription and amplified by PCR for construction of sequencing libraries.