GSM1908804: Bcat-02; Mus musculus; RNA-Seq
Sample 1-24: total RNA was extracted using Arcturus PicoPure RNA Isolation Kit (Applied Biosystems) and mRNA was selected by poly-A selection. Sample 25-29: total RNA was extracted using the Qiagen RNeasy Kit followed by DNaseI treatment. Sample 1-24: 3' end of poly A transcripts were amplified by PCR using NuGen Ovation 3'-DGE System Kit and libraries were prepared using NuGen Ovation SP+ Ultralow DR Multiplex System. Sample 25-29: cDNA libraries were generated using the Nugen Ovation RNA-Seq system V2 and libraries were generated using the NEBNext Ultra RNA Library prep kit for Illumina.