GSM1316406: Kasumi1_siRE_ATGTCA_rep3; Homo sapiens; RNA-Seq
Kasumi-1 cells were transfected with 200 nM siRNA using a Fischer EPI 3500 electroporator (Fischer, Heidelberg, Germany). RNA samples from three experimental replicates per condition were processed using the Tru-seq RNA Sample Prep Kit v2 from Illumina according to manufacturer’s protocol. Libraries were run in 4x multiplex on an Illumina Hiseq 2000 sequencer generating ~90 million paired-end reads per sample