GSM3102857: 48hr BFT2 rep 2 [RNA-seq]; Homo sapiens; RNA-Seq
Cells were washed once with PBS, collected using a cell scraper, and RNA was extracted from cells using the Qiagen RNeasy mini kit. After RNA extraction, the RNA pellet was flash frozen using 100% ethanol and dry ice. Samples were then stored at -80ºC. For library preparation, samples were removed from storage, and mRNA was enriched using the NEBNext Poly(A) mRNA Magnetic Isolation Module. Afterwards, a non-directional RNA-seq library was constructed using the NEBnext Ultra RNA Library Prep kit from Illumina. The 2nM pooled RNA-seq library was sequenced using the Illumina HiSeq.