GSM4009114: Dendrobium officinale protocorm; Dendrobium officinale; miRNA-Seq
Total small RNA was extracted by QIAGEN miRNeasy Mini Kit (QIAGEN, USA) according to the manufacturer's instructions. RNA concentration and purity were measured on a NanoDrop ND-2000 UV-Vis Spectrophotometer (Thermo Fisher Scientific, USA). RNA integrity was confirmed by denaturing agarose gel electrophoresis Small RNA sequencing libraries were prepared using TruSeq Small RNA Sample Prep Kit (Illumina, USA). Briefly, the RNA fragments were firstly ligated to 5'- and 3'-adapters using T4 RNA Ligase, and reverse-transcribed with miScriptII RT-Kit (Qiagen, Germany). The resultant cDNA fragments were electrophoresed on a 4% agarose gel. Bands between 140 and 160 bp were excised and extracted by MinElute Gel Extraction Kit (Qiagen, Germany)