GSM2157514: UT-R2; Mus musculus; RNA-Seq
Cancer cells were lysed on culture dishes and RNA was extracted using TOTLA RNA I etraction kit (Omega-Biotek). Total RNA was treated with DNase (ThermoFisher RapidOut) prior to library preparation. Directional RNA-seq libraries were prepared from 500ng total RNA using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs).