GSM4569826: eRGMc2_2iL_day12_unsortedControl; Mus musculus; OTHER
Genomic DNA was isolated from isolated populations using the Quick-DNA microprep plus kit (Zymo Research, #D3020) or a DNeasy blood and tissue kit (Qiagen, #69504) gRNAs from each population were amplified from genomic DNA using custom primers with the P7 flow cell overhangs: 5'-CAAGCAGAAGACGGCATACGAGATNNNNNNNNGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTTCTACTATTCTTTCCCCTGCACTGT-3' (8bp Barcode) and P5 overhang: 5'-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTTTGTGGAAAGGACGAAACACCG-3' using Q5 Hot Start High-Fidelity polymerase (NEB, #M0494S) for 21-24 cycles. sgRNA amplicons were purified with SPRI beads (Beckman Coulter, #B23318) PCR with Illumina primers