Vicia amurensis transcriptome
Total RNA was extracted from V. amurensis leaves using an RNeasy Plant Mini kit (Qiagen, Valencia, CA, USA) following the manufacturer’s instructions. mRNA was purified using the PolyATract mRNA Isolation System IV (Promega, Madison, WI, USA), and the purified products were used to synthesize full-length cDNAs using a ZAP-cDNA Synthesis kit (Stratagene, Santa Clara, CA, USA). Approximately 1 µg of cDNA was used to generate a DNA library for use with the Genome Sequencer (GS)-FLX Titanium System (Roche, Mannheim, Germany).
Vicia amurensis transcriptome