GSM2500953: NuGEN CA2 LCM QIAGEN; Mus musculus; RNA-Seq
RNA was extracted using either the QIAGEN RNeasy® Micro kit (cat #74004) or the Picopure® RNA isolation kit (Thermo Fisher Scientific, cat #KIT0204) according to the manufacturer's instructions. All samples were subjected to on-column DNase-treatment (Qiagen, cat#79254). Libraries were generating using either the NuGEN Ovation Universal RNA-Seq Systems 1-16 for Model Organisms (mouse, cat# 0348) or Clontech SMARTer Stranded RNA-Seq Kit (cat# 634837) with the Clontech RiboGone mammalian kit (cat# 634847) according to the manufacturer's instructions.