SLC6A15 targeted pooled resequencing, 100 individuals, major depressive disorder
Pooled DNA of 100 patients with major depressive disorder, 500ng DNA for library construction;  Barcoded standard fragment libraries were constructed using the Applied Biosystems protocol: SOLID Fragment Library Barcoding Kit Module 1-16; Libraries  were quantified by Qubit High Sensitivity dsDNA kit on Qubit 1.0 fluorometer; Libraries were pooled equimolar and used for bead preparation; Bead preparation: EZ bead system; SOLiD FWD SR sequencing chemistry  for single F3 tag; Exact Call Chemistry (ECC) was used and basespace reads were outputted by sequencer;
Cases_pool1