RNA-Seq from human NK cells
mRNA was captured using poly-dT oligos and reverse-transcribed into full-length cDNA using the described template-switching oligo.33,34 cDNA was amplified by PCR, purified using AMPure XP magnetic beads (Beckman Coulter). 1ng of cDNA was used to prepare a standard NextEra XT sequencing library (NextEra XT DNA library prep kit and index kits; Illumina). Barcoded Illumina sequencing libraries (Nextera; Illumina) were generated utilizing an automated platform (Biomek FXP, Beckman Coulter).
004_PBC_16_CD49a_neg