Illumina HiSeq 1500 sequencing; ATAC-seq experiment in two versions of Raji cells with inducible BZLF1 variants prior to and after induction of EBV's lytic cycle by doxycycline.
ATAC-seq experiment in two versions of Raji cells with inducible BZLF1 variants prior to and after induction of EBV's lytic cycle by doxycycline.
Replicate 3 Raji iBZLF1 full-length high (15 h)_s
Cells were cultivated and harvested as described in Schaeffner et al. 2019, (https://www.biorxiv.org/content/10.1101/317354v1). Modification of nuclear DNA was done after permeabilization and transposase processing as described in detail (https://www.biorxiv.org/content/10.1101/317354v1). Cells were either left untreated or were induced with Doxycycline of a final concentration of 100 ng/ml for 15 h. DNAs were isolated as described in Schaeffner et al. 2019 (https://www.biorxiv.org/content/10.1101/317354v1). Libraries were constructed as described in Schaeffner et al. 2019 (https://www.biorxiv.org/content/10.1101/317354v1).