GSM2343026: BY4741_2; Saccharomyces cerevisiae; OTHER
Extracts from UV-crosslinked yeast cells were used to isolate RNAs cross-linked to a tagged protein in a 2-step purification protocol under denaturing conditions. RNAs were partially digested. Sequencing 3' adapter and 5' adapter were ligated while protein-RNA complexes were bound to Ni-NTA agarose. RNA library was reverse transcribed and PCR amplified.