GSM2498230: adipocyte_KD_rep3; Homo sapiens; RNA-Seq
RNA samples were extracted using Trizol  (Invitrogen) according to the manufacturer's instructions. Extracted RNA samples underwent quality control (QC) assessment using the Agilent Bioanalyzer (Agilent, Santa Clara, CA) With a minimum of 1 µg input RNA, we generated first-strand cDNA using random hexamer-primed reverse transcription, followed by second-strand cDNA synthesis using RNase H and DNA polymerase, and ligation of sequencing adapters using the TruSeq Stranded RNA Library Prep Kit (Illumina, San Diego, CA). Fragments of ~350 bp were selected by gel electrophoresis, followed by 15 cycles of PCR amplification. The prepared libraries were then sequenced using Illumina’s HiSeq 2000.