GSM1545625: Run1 No Sepsis T0 n34; Homo sapiens; RNA-Seq
Blood was collected in EDTA tubes at the time of initial blood culture, and immediately placed on ice. Plasma and buffy coat were separated and two 1-ml aliquots transferred into bar-coded cryovials at -20oC until they were transferred to a secure, alarmed -80oC freezer. cDNA libraries were prepared from total RNA according to the TruSeq Stranded Total RNA Sample Prep Kit with Ribo-Zero sample preparation guide (Illumina). Unique adapter indexes (Illumina) were attached during sample prep and samples were run pooled and loaded into a single flow cell lane to reduce technical variability.