GSM2157793: SMRT_6mA; Arabidopsis thaliana; OTHER
Nine-day-old wild-type seedlings grown on MS plates were harvested and genomic DNA was extracted using the DNeasy Plant Mini Kit (Qiagen) according to the manufacturer’s instructions. The DNA libraries have been prepared following the PacBio instructions and sequenced on 13 SMRT cells using Pacific Biosciences RSII sequencing technology (Pacific Biosciences). The SMRT bell was produced using the DNA Template Prep Kit 1.0 (Pacific Biosciences). SMRTbell templates were bound to polymerases by using the DNA/Polymerase Binding Kit P6 v2 (Pacific Biosciences). 10 μg of Arabidopsis genomic DNA were mechanically sheared to an average size distribution of 10 kb.  Polymerase-template complexes were bound to magbeads by using Magbead Binding Kit (Pacific Biosciences), and sequencing was carried out on the PacBio RSII by using the DNA Sequencing Kit 4.0 (Pacific Biosciences). Movie lengths were 240 min for each of 13 SMRT cells. Subread filtering was performed by using Pacific Biosciences’ SMRT analysis software (v2.3.1). SMRT sequencing