GSM2772446: JK CD63Flow 1; Homo sapiens; ncRNA-Seq
Trizol (Invitrogen) and RNaseazy (Qiagen) kits were used to extract total RNA according to the manufacturer's instructions. Small RNA sequencing libraries were size selected (<150bp) using the Agencourt AMPure beads. KAPA qPCR Library Quantification Kit was applied prior to pooling for the final libraries validation and normalization. The sequencing was done with 12.5 million reads per sample with 75 bp reads. (Multiplexing 32 samples on a 400 million read flow cell).