RNA-Seq of mus musculus: lymph node macrophages with free pIC - mouse 1
10ng of Macrophage RNA was used as input to a Clontech Smart Seq v4 kit (Clontech labs, Mountain View, CA) to generate double stranded cDNA per the manufacturers instructions. The cDNA was quantified using the Qubit HS DNA kit (Thermo Fisher Scientific) and 1 ng was used to prepare libraries using a Nextera XT DNA library preparation kit (Illumina, San Diego, CA) per the manufacturers instructions. The libraries were quantified using a Qubit Fluorimeter (Thermo Fisher Scientific) and multiplexed samples were run on a HiSeq 2500 instrument (Illumina). Each library was sequenced for 2×40 million of 100-nucleotide reads.
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