GSM3763089: TG_E15; Mus musculus; ChIP-Seq
Samples were sheared in 1×Hank's balanced salt solution (HBSS) and fixed in 1% formaldehyde to link proteins to DNA. Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Specific antibody of H3K9me2 was used in immunoprecipitation and genomic DNA was purified. All procedures followed the guidelines provided by Illumina Paired-End DNA Sample Prep Kit. Briefly, after purified from ChIP samples, DNA was end repaired and then added with A and adapter for amplification.