GSM1642985: NL4_MIGS; Homo sapiens; MBD-Seq
Genomic DNA was purified using the Qiagen DNEasy kit. Genome-wide DNA methylation was mapped using MBD-isolated genome sequencing (MiGS) (Serre, 2010), with the following differences. DNA (10 ug) was sheared on a Covaris S220 to an average fragment size of 120 bp and methylated fragments were purified on PrepEase DNA columns (USB). Methylated DNA sequences were captured with the methyl binding transcriptional repressor protein MBD2 MethylMiner Methylated DNA Enrichment kit #ME10025 (Invitrogen). A sequencing library for each sample was prepared from the immunoprecipitated DNA using Ilumina’s ChIP-seq DNA Sample Prep Kit and the quality of the DNA was assessed on an Agilent Bioanalyser.