GSM2500340: Male Rep6; Rattus norvegicus; RNA-Seq
Total RNA was isolated using the miRNeasy mini kit (Qiagen, Valencia, CA) according to the manufacturers protocol with the on-column DNase digestion. RNA concentration was determined using the Qubit RNA BR Assay (Invitrogen, Carlsbad, CA) and RNA integrity was analyzed with the Agilent Bioanalyzer (Agilent, Santa Clara, CA) with all of the RNA samples used for sequencing having a RNA integrity number (RIN) of at least 9. Ribo-zero gold depleted paired-end sequencing libraries were then constructed from 650ng total RNA using the TruSeq Stranded Total RNA Sample Kit (Illumina, San Diego, CA) according to the manufacturers protocol.