P10T
DNA samples were first sheared with the Covaris M220 system (Covaris) using the manufacturers recommended settings. Subsequent end-repair, adenylation, paired-end adaptor ligation and PCR amplification of the sheared DNA samples were performed according to the Agilent SureSelect XT Target Enrichment protocol, using reagents from the SureSelect XT Library Prep Kit. Exonic regions of the DNA were enriched using the SureSelectXT Human All Exon v6 (60 Mb) kit (Agilent Technologies) according to manufacturers protocol. Amplification of the captured libraries were then performed and the quantity and quality of the enriched DNA libraries determined on the Bioanalyzer 2100 (Agilent Technologies). Finally, the exome-enriched libraries were sequenced on Illuminas HiSeq sequencing platform to generate 150-bp paired-end reads.
P10T