GSM3703342: K562_1; Homo sapiens; ChIP-Seq
Chromatin was sheared using Bioruptor® Pico sonication device (Diagenode Cat# B01060001) to an average of 150-200 bp fragments as assessed by High Sensitivity NGS Fragment Analysis Kit (DNF-474) on a Fragment Analyzer™ (Advanced Analytical Technologies, Inc.). ChIP was performed manually with the iDeal ChIP-seq kit for Histones (Diagenode Cat# C01010059) Libraries were prepared using IP-Star® Compact Automated System (Diagenode Cat# B03000002) from input and ChIP'd DNA (starting amount 1ng) using MicroPlex Library Preparation Kit v2 (12 indices) (Diagenode Cat# C05010013). Library amplification is assessed using High Sensitivity NGS Fragment Analysis Kit (DNF-474) on a Fragment Analyzer™ (Advanced Analytical Technologies, Inc.). Libraries were then double size-selected and purified using Agencourt® AMPure® XP (Beckman Coulter) and quantified using Qubit™ dsDNA HS Assay Kit (Thermo Fisher Scientific, Q32854). Finally their fragment size was analyzed by High Sensitivity DNA Analysis Kit on a 2100 Bioanalyzer system (Agilent).