GSM4010559: 2cell_late_total_RNA_Con_rep1; Mus musculus; RNA-Seq
8-week-old female mice were intraperitoneally injected with PMSG and hCG. Embryos were harvested in M2 medium 23 h after hCG Total RNA-seq libraries were prepared as previously described (Brind'Amour, J., et al. 2018). Total RNA was isolated from about 100 mouse embryos using TRIzol (Thermo, 10296028), and ribosomal RNA was depleted using the NEB Next rRNA Depletion Kit (NEB, E6310S) according to the manufacturer's instruction. Double-stranded cDNA was synthesized using NEB Next first-strand (NEB, E7771S) and second-strand synthesis modules (NEB, E7550S). Library preparation was performed using NEBNext UltraTM II DNA Library Prep Kit (NEB, E7645S) according to the manufacturer's instruction. All libraries were sequenced by Illumina Hi-Seq Xten platform according to manufacturer's instruction.