RNA-seq of synovial tissue: Sample S033_L5Q1_I23
scRNA-seq was performed using the CEL-Seq2 protocol. Libraries were sequenced on a Hiseq 2500 (Illumina) in Rapid Run Mode to generate paired-end reads. Cell barcodes in the reverse reads were used to demultiplex the forward reads into a separate fastq file for each cell, with the six nucleotide UMI from the reverse read copied to the header of the forward read.
S033_L5Q1_I23