GSM3703288: SSRP1dsRNA_a_RNAseq_Rep2; Drosophila melanogaster; RNA-Seq
At the end of the 4-day knockdown, Drosophila S2 cells were harvested. Human HEK293T cells were added to the harvested S2 cells (0.8x10e6 human cells / 15x10e6 S2 cells) as spike-in control.  Each Pool of S2 and HEK293T cells was washed twice using 1 X PBS. Total RNA was extracted using the QIAGEN RNeasy mini kit. Libraries were prepared using the Illumina TruSeq Standard mRNA LT sample prep kit and sequenced on the Illumina HiSeq 2500 platform. RNA-seq libraries were generated from 1ug of high quality total RNA, as assessed using the Bioanalyzer (Agilent). Libraries were made according to the manufacturer's directions using the TruSeq Stranded mRNA LT Sample Prep Kit – set A (Illumina, Cat. No. RS-122-2101) kit. The resulting short fragment libraries were checked for quality and quantity using the Bioanalyzer (Agilent) and Qubit Fluorometer (Life Technologies). Libraries were pooled, re-quantified and sequenced as 50 bp single reads on the Illumina HiSeq 2500 instrument.