GSM1971054: K4me3_Std_10ng; Mus musculus; ChIP-Seq
Cells were crosslinked with 1% formaldehyde for 10 min at 37◦C; then, crude nuclei were purified. Chromatin was fragmented by sonication with a Bioruptor UCD-300 (Diagenode) to obtain fragments 200–800 bp in length. For each ChIP assay, 2–5 ug of antibodies were added and incubated at 4◦C overnight. ChIP DNA was quantified with a Qubit fluorometer using the Quant-iT dsDNA HS assay kit ChIP-seq libraries were generated following the the manufactorer's instructions (Illumina ChIP-SEQ. DNA Sample prep. Kit, #1003473, lot#6330828)