GSM3702455: WT_non_irradiated_75_Ileum; Mus musculus; RNA-Seq
total RNA was extracted from 0.1-1*106 sorted cells with RNeasy Micro Kit (Qiagen). 100 ng of total RNA was processed using the TruSeq Stranded Total RNA HTSample Prep Kit (with Ribo-Zero Gold) of Illumina (RS-122-2303). Sequencing libraries were constructed with barcodes to allow multiplexing of 24 samples ran on 3 lanes. ~20 million single-end 50-bp reads were sequenced per sample on Illumina HiSeq 2500 high output mode instrument.