GSM2349276: wt_eye_1; Mus musculus; RNA-Seq
Tissues were harvested immediately after euthanasia, snap frozen in liquid nitrogen and stored at -80˚C. Tissues were subsequently pulverized in liquid nitrogen and homogenized in TRIzol. Embryonic Mouse Fibroblasts (MEF, passage 2) were cultured in 10cm dishes in normal conditions (37˚C, 5% CO2) and homogenized directly in Trizol. Standard illumina protocols were used to make libraries (TruSeq RNA Sample Preparation Kit v2)