GSM1138345: degradome-orange-L; Citrus sinensis; RNA-Seq
All samples were collected and frozen in liquid nitrogen immediately after collection and kept at -80℃ until use.Total RNA was extracted using Trizol reagent. The 3’ fragment generated by RISC (RNA-induced silencing complex), which contains a 5’-monophosphate, was ligated to a 5’ RACE adaptor. Reverse transcription is then performed using oligo(dT) as a 3’ adaptor and the cDNAs were formed by second strand synthesis.