GSM3703431: Prog_treated_with_Luciferase: Lineage-CD34+CD38+ progenitors treated with Luciferase for 14 days; Homo sapiens; RNA-Seq
Single cells were sorted in a BD Influx cytometer and were pelleted by centrifugation followed by resuspension in DMEM at approximately 500cells/µl. Cell viability and concentration was verified by Countess cell counter. Each sample was loaded into one well of a Chromium chip (10X Genomics, #120236), following manufacturer's instructions, and aiming for a recovery of 5,000 cells per sample. Library construction was carried out according to the manufacturer's instructions and were sequenced on Illumina Hiseq 2000.