GSM3703838: DH2F: 39mCHAVI009; Homo sapiens; RNA-Seq
Placental biopsy samples in RNAlater from HIV-infected Malawian women were thawed on ice. Fifty mg of tissue was homogenized in Trizol (Invitrogen), and total RNA was purified with miRNeasy extraction kit (Qiagen) according to the manufacturer guidelines. Total RNA libraries were prepared from 500 ng purified RNA using TruSeq Stranded Total RNA with Ribo-Zero Gold Kit (Illumina).