Illumina HiSeq 2000 paired end sequencing; Finding new Chimeric RNAs in RNA-seq data, a test study in Acute Myeloid Leukemia patient cells
Finding new Chimeric RNAs in RNA-seq data, a test study in Acute Myeloid Leukemia patient cells
om110223_p
Three AML samples (AML-Normal-Karyotype, AML-t(15;17) and AML-inv16) taken from patients OM100011, OM110223 and OS110089, respectively from the Biological Resources Center, CHU-Nîmes, France, were selected for RNA-seq experiments. Peripheral Blood Nononucleated Cells (PBMC) from total blood (OS110089) or Bone marrow cells (OM100011, OM110223) were isolated by density gradient centrifugation on Ficoll. Total RNA was isolated using mini RNeasy kit (Qiagen) according to the manufacturer instructions. The quantity and quality of extracted RNA was evaluated using the NanoDrop ND-1000 spectrophotometer and the Agilent 2100 BioAnalyzer. A poly-adenylated stranded cDNA library was created for each sample from 4 µg of total RNA (performed under contract by GATC biotech using proprietary protocol).