GSM3761283: 3080_wt_1; Mycobacterium tuberculosis; RNA-Seq
RNA was prepared from 30mL of log-phase M. tuberculosis using Trizol reagent after bead beating to lyse cells. RNA was DnaseI treated using the TURBO DNA-free kit from Thermo-Fisher. Ribo-zero was used to deplete ribosomal RNA. Then the RNA library was prepared using standard Illumina protocols.