Single_Cell RNA-Seq of Mus Musculus: adult Tas1r3+ taste cells: Sample RM_Taste_SCRS1
"Individual Tas1r3 expressing taste cells were isolated from circumvallate taste papillae of Tas1r3-GFP transgenic mice. . Single cell mRNA was amplified using the aRNA method using the TargetAmp 2-Round aRNA Amplification Kit 2.0 (catalog no. TAU2R51224; Epicentre, Madison, WI). Unstranded sequencing libraries were prepared using the NEBNext mRNA LibraryPrep Master Mix Set for Illumina (catalog no. E6110; New England Biolabs, Ipswich, MA).Three libraries with indices 4, 6 and 12 were multiplexed in equmolar concentration and 100bp paired end sequencing was dome on a HiSeq 200 sequencer. Raw sequences in FastQ format were aligned to reference mouse genome (version GRCm38.p3) using the STAR aligner with default settings using Gencode M4 as the junction annotation file."
Tas_9