The Next Generation of Target Capture Technologies - Large DNA Fragment Enrichment and Sequencing Determines Regional Genomic Variation of High Complexity
Five micrograms of enriched, amplified material were used as input for preparation of the sequencing library. The library was prepared for sequencing using the Illumina Paired-End DNA Sample Prep Kit (Cat. # PE-102-1001), according to the manufacturer’s protocol. Sequencing was performed using an Illumina Genome Analyzer II, 2x 125 base paired-end chemistry.