Salsola soda gene expression of developmental stages
Total RNA of five different developmental stages of Salsola Soda, 1) young seedling, 2) cotyledons, 3) first leaf pair, 4) second leaf pair, 5) old leaf pair, was isolated with RNeasy Plant Mini Kit (Qiagen) following Standard protocol (January 2011) and including DNase Digestion with RNase-Free DNase Set (Qiagen). 500 ng were used for cDNA library generation conducted with TruSeq RNA Sample Preperation Kit (Illumina Inc.)  following Low Sample Protocol (TruSeq RNA Sample Preparation v2 Guide, Illumina Proprietary, Part # 15026495 Rev. C, May 2012). Sequencing of single reads was performed on an Illumina HiSeq2000 platform.
Ssoda_yS_2