GSM2232704: 35450-BM-ASXL2; Homo sapiens; RNA-Seq
BM cells were harvested, Lineage-ckit+ScaI+ fractions were sorted by flow cytometry, and RNA was harvested using RNAeasy micro kit. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols