GSM2845258: scRNA_seq_FoxD3_Citrine_65; Danio rerio; RNA-Seq
FACS sorted cells were washed with PBS and stored at -80C in lysis buffer. RNA was extracted using Ambion RNAqueous Micro Total RNA isolation kit (AM1931), checked on Bioanalyser, samples with RIN >7 were used to prepare cDNA using Takara Clontech SmartSeq2 V4 kit (634889). Sequencing libraries were prepared using Illumina Nextera XT library preparation kit (FC-131-1024). For scRNA.seq, individual cells were collected by FACS, cDNA was obtained and sequencing libraries as previously described (Picelli et al., 2014). Libraries were sequenced using 50 bp single end reads for 96 cells. 4x10E7 dilution of ERCC spike in control was used.