3
At with 7 days 18C white light
Library prepared by Illumina-compatible NEBNext UltraTM Directional RNA Library Prep Kit.200 ng RNA used to generate first strand of mRNA in the presence of Actinomycin D. dCDNA was purified HighPrep magnetic beads. It was subjected to 15 cycles of PCR for 30 sec. PCR product was purified and sequencing library was quantified by Qubit fluorometer.
3
SUB5761791
3