LIB107643
PacBio HiFi WGS of Vespa mandarinia: worker female thorax
High molecular weight (HMW) DNA extraction was performed from the thorax using the Qiagen MagAttract HMW DNA Kit. DNA shearing was performed using the Diagenode Megaruptor 2 using a 20 kb fragment protocol. The sheared DNA was prepared for PacBio sequencing using the SMRTBell Express Template kit 2.0. The final library was fractioned on a Sage Science SageELF device, and fractions were combined from 10 kb  18kb to generate the final library for sequencing. Sequencing was performed on a Sequel II System using Bind Kit 2.0, Sequencing kit v2.0, and SMRT Cell 8M.  To target HiFi reads, the library was loaded at 100pM concentration and run using 30 hour movie times on two cells.  Raw subreads were converted to HiFi data by processing with CCS to call a single high quality consensus sequence for each molecule, using a 99.5% consensus accuracy cutoff.
LIB107643
SUB7853537
LIB107643