AmB-Persister
RNA-Seq of high dose of AmB-treated C. albicans adherent cells
Overnight cultured C. albicans TDH3-GFP-CAI4 cells were adjusted to 106 cells/ml using synthetic medium plus dextrose medium and supplemented with 80 M farnesol. 200 l of aliquot was dispended into wells of 96-well plates (NEST). After 36 h incubation at 37 C with 100 rpm rotation, supernatants were discarded and wells were thoroughly washed three times with PBS to remove the non-adherent cells prior to antifungal challenge. The culture media containing 20 g/ml of amphotericin B was added to wells and the plates were incubated at 37 C with 100 rpm rotation for additional 24 h. Then supernatants were aspirated and the wells were washed twice. The adherent cells were scraped and collected for RNA extraction following with RNA sequencing.
AmB-Persister
SUB4143687
AmB-Persister