MossDim2016.2.25.HylSpl.R
16S V4V5 amplicon sequencing of Hylocomium splendens: Site 2 (near Fairbanks, AK, USA)
The V4-V5 region of the 16S ribosomal RNA gene was amplified using 515f/806r primers (Caporaso et al. 2011). Each primer combination had a unique barcode corresponding to a unique sample. Barcodeded amplicons were normalized and pooled into a single library which was sequenced on a 2x150 Illumina MiSeq run. Sequences were were subsequently demultiplexed by barcode into individual files corresponding to each sample.
MossDim2016.2.25.HylSpl.R
SUB7236662
MossDim2016.2.25.HylSpl.R