GSM3445731
GSM3445731: ChIRTseq_TERRAwt; Mus musculus; OTHER
Reverse crosslinking was achieved by addition of 20 ml of 5M NaCl and incubation at 65ºC for 8 hours. DNA was recovered by RNase and proteinase K treatment, phenol/chloroform extraction and ethanol precipitation. 0.5ng of DNA per sample were used for the CHIRT-seq experiment (5ng for the INPUT). Samples were processed through subsequent enzymatic treatments for fragmentation (5 min), end-repair, dA-tailing, and ligation to adapters with "NEBNext Ultra II FS DNA Library Prep Kit for Illumina" from New England BioLabs (catalog # E7805). Adapter-ligated libraries were completed by limited-cycle PCR and extracted with a [single] double-sided SPRI size selection. Resulting average fragment size is 400 bp from which 120 bp correspond to adaptor sequences. Libraries were applied to an Illumina flow cell for cluster generation and sequenced on an Illumina NextSeq 500 sequencer, by following manufacturer's protocols.
GEO
GSM3445731
GEO Accession
GSM3445731