WT4P4
Polycomb protein SCML2 associates with USP7 and counteracts histone H2A ubiquitination in the XY chromatin during male meiosis
Total RNAs were extracted from 4-month-old wild type and Scml2-/Y testes using TRIzol reagent. Polyadenylated RNAs were isolated from 5 μg of total RNAs with the NEBNext poly(A) mRNA magnetic isolation kit (New England Biolabs, E7490S). RNA-seq libraries were prepared with NEBNext Ultra RNA Library Prep kit (New England Biolabs, E7530S) and NEBNext Multiplex oligos for Illumina (Index Primers Set 1) (New England Biolabs, E7335S). A total of four libraries (two mice per genotype) were constructed, each with a unique index primer (Table S1). Sequencing of the multiplex libraries was done in a 100 bp single-end sequencing format on Illumina HiSeq 2000.
WT4P4
University of Pennsylvania
WT4P4