sample1
Soilsample
The amplicon libraries were prepared according to Illumina protocol “16S Metagenomic Sequencing Library Preparation, Part # 15044223 Rev. B” (Illumina) using Phusion polymerase (Thermo) instead of KAPA HiFi. Amplicon primers are 5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG and 5’-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC according to 38. For indexing Nextera XT Index kit (FC‐131‐1001) was used. Samples were quantified using Quant-It DNA HS kit (Invitrogen), pooled in equal amounts and quality controlled using a Bioanalyser (Agilent). Paired end sequencing was performed in an Illumina MiSeq sequencer with MiSeq Reagent Kit v3 (2 x 300pb).
34
AIT Austrian Institute of Technology GmbH
sample1