MD2017.16.30.AulPal
16S V4V5 amplicon sequencing of Aulacomnium palustre: Site 16 (near Anchorage, AK, USA)
The V4-V5 region of the 16S ribosomal RNA gene was amplified using 515f/806r primers (Caporaso et al. 2011). Each primer combination had a unique barcode corresponding to a unique sample. Barcodeded amplicons were normalized and pooled into a single library which was sequenced on a 2x150 Illumina MiSeq run. Sequences were were subsequently demultiplexed by barcode into individual files corresponding to each sample.
MD2017.16.30.AulPal
SUB7236662
MD2017.16.30.AulPal