SKNEP1-LEE011Resistant
WES of human Ewing sarcoma cell line SKNEP1 derived to be resistant to LEE011
Picogreen dsDNA quantitation was performed on each sample to determine total yield. Next, 100 ng of gDNA from each sample was taken to fragmentation and library construction. Prior to library construction, DNA was fragmented (Covaris sonication) to 250 bp and further purified using Agentcourt AMPure XP beads. Size-selected DNA was ligated to sequencing adaptors with sample-specific barcodes using the Kapa HTP library construction kit (Kapa Biosystems).  Sample-specific barcodes were selected according to Illumina low-plexing guidelines. All samples were of good quality. Libraries were pooled and sequenced on an Illumina MiSeq to estimate the concentration based on the number of index reads per sample. Library construction is considered to be successful if the yield is > 250 ng. All samples yielded sufficient library. Libraries were pooled in equal mass to 750 ng (3-plex) and then hybrid capture was performed using SureSelect Human All Exon V5 and the Agilent SureSelect system. The capture was sequenced on one lane of the HiSeq2500 in Rapid Run mode.
SKNEP1-LEE011Resistant
SUB4674100
SKNEP1-LEE011Resistant