SP1
Streptococcus pneumoniae
Bacteria were grown until exponential phase in LB broth. DNA was isolated from the bacteria with the DNeasy kit (QIAGEN) following the instructions of the manufacturer. Yield of double-stranded DNA was quantified with the Qubit spectrofluorimeter (Invitrogen). Aliquots of five ng of each bacterial DNA preparation were added to 315 ng human DNA in a total volume of 130 µl low TE-buffer (Life Technologies).
SP1
SUB1545693
SP1