Pb204N1
Whole genome shotgun sequencing of Enterobacter xiangfangensis Pb204
The library was prepared using Nextera DNA Sample preparation kit (Illumina) following the manufacturer's user guide. The intial concentration of DNA was evaluated (Table 1) using the Qubit dsDNA HS Assay Kit (Life Technologies). 50 ng DNA was used to prepare the library. The sample underwent the simultaneous fragmentation and addition of adapter sequences. These adapters are utilized during a limited-cycle (5 cycles) PCR in which unique indices were added to the sample. Following the library preparation, the final concentration of the library (Table 1) was measured using the Qubit dsDNA HS Assay Kit (Life Technologies), and the average library size (Table 1) was determined using the Agilent 2100 Bioanalyzer (Agilent Technologies).  The library was diluted (to 10.0pM) and sequenced paired end for 300 cycles using the HiSeq system (Illumina).
Pb204N1
SUB4684019
Pb204N1