GSM4512357
GSM4512357: SHR-AA; Rattus norvegicus; RNA-Seq
The whole mesenteric artery or aorta were cut into fine pieces and digested with 2 mg/ml collagenase I  and 2 mg/ml dispase II in Hank's balanced salt solution containing calcium and magnesium for a total 40 min. The remaining aortic media and endothelium were cut open longitudinally and digested with 1 mg/ml papain , 0.5 mg/ml 1,4-Dithioerythritol, 0.156 mg/ml Taurine; 0.25 mg/ml bovine serum albumin in PBS. Single nucleated live cells were sorted into PBS with 0.04% BSA using a flow cytometer and were then analyzed on a 10x Chromium instrument using Chromium Single Cell 3' Reagent Kit v2 chemistry (10X Genomics), following a standard protocol. Library was generated and sequenced on a NovaSeq platform (Illumina) with paired-end 150 bp (PE 150) sequencing strategy. Single-cell RNA-seq
GEO
GSM4512357
GEO Accession
GSM4512357