GSM4512965
GSM4512965: WT, 5 weeks of age uterus, replicate 4; Mus musculus; RNA-Seq
Tissue slices were homogenized using a BioMasher®II (Wako Pure Chemical, Osaka, Japan) or TRIzol Reagent (Invitrogen) prior to purification of total RNA using an RNeasy® Mini Kit (QIAGEN, Hilden, Germany) or SV Total RNA Isolation System (Promega) according to the manufacturer's protocol. cDNA libraries were generated using a NEBNext Ultra RNA Library Prep Kit (New England BioLabs, Beverly, MA, USA). Sequencing was performed using HiSeq1500 (Illumina) with a single-read sequencing length of 60bp. Cluster amplification and 150 bp paired-end sequencing was performed according to the manufacturer's protocol (Illumina) for NovaSeq 6000 (Illumina).
GEO
GSM4512965
GEO Accession
GSM4512965