GSM3445043
GSM3445043: shRai1_TTX_rep3; Mus musculus; RNA-Seq
Cells were homogenized in TRI Reagent BD (Sigma) and RNA isolated by phenol-bromocholoropropane extraction. RNA was fragmented and adapters were ligated directly to 3' end of RNAs. Nascent RNA was isolated using bromouridine-antibody-conjugated beads (Santa Cruz), and library preparation was derived from an adaptation of the Illumina Tru-seq protocol.
GEO
GSM3445043
GEO Accession
GSM3445043