T2C in mouse fetal liver rep2
T2C in mouse fetal liver rep2
T2C was performed in duplicates as described (Kolovos et al., 2014, 2018). In brief, ~10 million cells were isolated, following cross-linking in 1% paraformaldehyde (10 min, 20C; Electron Microscopy Science), chromatin was cut using ApoI or HindIII (New England Biolabs), and ligated in dilution under conditions that allowed most nuclei to remain intact.
T2C in mouse fetal liver rep2
SUB4407446
T2C in mouse fetal liver rep2