218-2
sample 218-2 at 0.05m depth from 2010-07-30 MiSeq run 2
The nifH gene was PCR-amplified from DNA extracts of sands from an oil-contaminated beach. For PCR amplification we used the nifH primers IGK3 (5'-GCIWTHTAYGGIAARGGIGGIATHGGIAA-3') and DVV (5'-ATIGCRAAICCICCRCAIACIACRTC-3') with Fluidigm common sequences on the 5' ends. Fluidigm indexes were incorporated and sequencing performed on the Illumina MiSeq with the addition of the custom Fluidigm sequencing primers to the reagent cartridge.
218-2
SUB4560456
218-2