GSM4399740
GSM4399740: N2_REP2; Caenorhabditis elegans; RNA-Seq
Total RNA was extracted using TRIzol Reagent. RNA quality (RIN) and quantity were assessed on the TapeStation 2200 (Agilent). Two rounds of mRNA purification were performed on 1ug total RNA using the Dynabeads mRNA DIRECT Kit (Invitrogen, 61011). First-strand cDNA was generated using the Superscript III First-Strand Synthesis System (Invitrogen, 18080051), followed by second-strand synthesis using DNA polymerase I (Invitrogen, 11917010). cDNA libraries were prepared using the Nextera XT DNA Library Preparation Kit (Illumina, FC-131-1024).
GEO
GSM4399740
GEO Accession
GSM4399740