GSM1820919
GSM1820919: Input_293T_B4N_N; Homo sapiens; ChIP-Seq
1.5 × 109 cells grown as monolayer cultures in 150mm dishes (100 plates total) were used for BioTAP-XL purification. Tetracycline was added (1 μg/mL) to 60-70 % confluent culture to induce transcription of the N- or C-BioTAP tagged BRD4-NUT cDNA clones for 16h. The main steps of BioTAP-XL procedure: harvesting cells, formaldehyde cross-linking, chromatin preparation, affinity purification, input and IP DNA recovery, and ChIP-seq library preparation, were performed as described (Alekseyenko et al. 2014a; Alekseyenko et al. 2015) ChIP-seq library preparation, were performed as described (Alekseyenko et al. 2014a; Alekseyenko et al. 2015)
GEO
GSM1820919
GEO Accession
GSM1820919