DNA-PIM001P-reg3
Whole exome sequencing of PDX tumor engrafted in mammary fat pad treated with vehicle: Sample DNA-PIM001P-reg3
"Libraries were prepared from 200ng of Biorupter ultrasonicator -sheared gDNA using the Agilent SureSelectXT Reagent Kit. Libraries were prepared for capture with ten cycles of PCR amplification, then assessed for size distribution on Fragment Analyzer using the High Sensitivity NGS Fragment Analysis Kit (Advanced Analyticals). Exon target capture was performed using the Agilent SureSelectXT Human All Exon V4 kit. Following capture, index tags were added to the exon enriched libraries using six cycles of PCR. Libraries were sequenced either one library per lane (5 samples) or equal molar concentrations of 3 libraries were pooled and sequenced in two lanes of the HiSeq4000 sequencer, using the 76nt paired end format."
DNA-PIM001P-reg3
TMC-phs001742
DNA-PIM001P-reg3
alignment_software
bwa 0.7.12-r1039