GSM3576734
GSM3576734: ESC timecourse_0-72hours; Mus musculus; RNA-Seq
RNA was purified and proccessed for indrop according to the protocol from Klein et al (Cell, 2015, doi:10.1016/j.cell.2015.04.044) RNA libraries were prepared for sequencing by the indrop core facility at Harvard Medical School. We used library version v3 where R1 is the biological read. R2 carries the first half of the gel barcode, R3 carries the library index and R4 the second half of the gel barcode, the UMI and a fraction of the polyA tail. Library index for LIB1 (ESC 0hr) is:ATAGAGAG , LIB2 (ESC 24hr) is: AGAGGATA, LIB3 (ESC 48hr) is:CTCCTTAC and LIB4 (ESC 72hr) is TATGCAGT.
GEO
GSM3576734
GEO Accession
GSM3576734