GSM3768320
GSM3768320: S08-LLC-SH21-GFP-1; Mus musculus; RNA-Seq
Total RNA was extracted from cells using RNeasy Plus Mini Kit (Qiagen), which includes a step to eliminate genomic DNA. RNA libraries were constructed using an Illumina TruSEQ stranded mRNA Sample Prep Kit (Cat# RS-122-2101). Total RNA was combined with RNA purification beads to bind PolyA RNA to oligodT magnetic beads. mRNA was eluted and converted to double stranded DNA. A-tailing, adapter ligation, and PCR amplification using 15 cycles was performed to complete the library construction.
GEO
GSM3768320
GEO Accession
GSM3768320