GSM4512353
GSM4512353: 5 Gy anti-NRP1 3; Mus musculus; RNA-Seq
total RNA was isolated using the Qiagen microRNeasy kit Libraries for RNA-Seq were constructed with Clontech Kit to generate strand-specific RNA-seq libraries.  The workflow consists of poly(A) RNA selection, RNA fragmentation and double-stranded cDNA generation using a mixture of random and oligo(dT) priming, followed by end repair to generate blunt ends, adaptor ligation, strand selection, and PCR amplification to produce the final libraries Amplified libraries were quantified by Qubit dsDNA HS (High Sensitivity) Assay Kit, and quality-checked by the Agilent 4200 TapeStation System. Different index adaptors were used for multiplexing samples in one sequencing lane. Sequencing was performed with NextSeq500 High Output sequencer to produce 75 base-pair single-end reads (1 x 75 bp).
GEO
GSM4512353
GEO Accession
GSM4512353