GSM3504375
GSM3504375: Control CM nucRNA-seq rep2; Mus musculus; RNA-Seq
RNA from Bead-bound PCM1+ nuclei was collected using the RNEasy Plus Micro kit (Qiagen). Nuclear RNA-seq libraries were constructed with the Stranded RNA-seq Kit with Ribo Erase (Kapa Biosystems) with custom Y-shaped adapters. Paired-end 2x75 bp sequencing was performed for RNA-seq libraries on an Illumina Nextseq instrument (DNA Link). Reads were first mapped to the mouse genome (mm10) using STAR. Differential expression analysis was then carried out with DESeq2.
GEO
GSM3504375
GEO Accession
GSM3504375