EcE
Euphilomedes carcharodonta embryos 454 transcriptome
mRNA was extracted from tissue using Trizol. cDNA was prepared using the SMARTer cDNA synthesis kit from Clontech. Amplified cDNA was purified using phenol:chloroform:isoamyl (25:24:1 v/v/v).
University of South Carolina
EcE