GSM1824028
GSM1824028: 12 days post-fertilization (dpf) embryo sample (MH_0015); Fundulus heteroclitus; miRNA-Seq
Total RNA from the different stages of embryos was isolated using STAT60 method (Tel-Test, Inc., Friendswood, Texas) and quantified using Nanodrop spectrophotometer. Quality of the total RNA was checked by running the samples on a Agilent Small RNA kit using 2100 Bioanalyzer. All the samples had RNA integrity number of >9.8. Small RNA library construction and microRNA sequencing were performed by the Genomic Services Lab at the HudsonAlpha Institute for Biotechnology in Huntsville, AL, USA. Small RNA libraries were prepared using the small RNA expression kit (SREK) protocol (Applied Biosystems, Foster City, CA). Sequencing was done on a SOLiD V3 system (Applied Biosystems).
GEO
GSM1824028
GEO Accession
GSM1824028