Snake Inclusion Body Disease experiment
Metagenomic analysis of snake inclusion body disease (IBD)
RNA from case and control tissues was extracted with Trizol and treated with DNase to remove DNA.  RNA was primed for RT with oligo-dT, then cDNA was converted into doubly end tagged dsDNA by primer extension with DNA polymerase.  These libraries were then amplified and the barcoded Illumina adapters added by PCR.  Barcoded libraries were normalized and pooled and size selected.  For a much more detailed protocol, please see the associated reference.
Snake Inclusion Body Disease Samples
UCSF
Snake Inclusion Body Disease experiment