GSM4200393
GSM4200393: Rpo47.H2O2.chip-exo.r3; Saccharolobus solfataricus; ChIP-Seq
Cells were lysed in lysis buffer (50 mM HEPES/NaOH pH 7.5, 140 mM NaCl, 1 mM EDTA, 0.1% Na-deoxycholate, 1% Triton X-100) and DNA was sheared by sonication. Lysates were cleared by centrifugation. Chromatin-immunoprecipitation was carried out with protein-A-purified polyclonal antibodies. Immunoprecipitated complexes were treated ChIP-exo protocol using the ChIP-exo kit from Active Motif based on the protocol published by Serandour et al. (Genome Biology 14:R147 (2013)
GEO
GSM4200393
GEO Accession
GSM4200393