CbA
Caecidotea bicrenata cave head 454 transcriptome
mRNA was extracted from tissue using the Nucleospin RNA XS kit (Macherey-Nagel). cDNA was prepared using the SMARTer cDNA synthesis kit (Clontech). Amplified cDNA was purified using phenol:chloroform:isoamyl (25:24:1 v/v/v).
University of South Carolina
CbA