GSM3399676
GSM3399676: RNApolII_chiapet_rep2; Mus musculus; ChIA-PET
Cells were lysed using SDS-based lysis buffer and sonicated, protein-DNA complexes were then isolated with antibody. Immunoprecipitated protein-DNA complex were ligated with linkers. The proximity ligated chromatin complex was eluted with 1% SDS and de-crosslinked using proteinase K and purified. The purified fragments were tagmented using the Nextera DNA Sample Preparation Kit (Illumina). The linker ligated ChIA-PET constructs were selected using streptavidin magnetic beads. The magnetic beads were blocked with yeast tRNA prior to the selection. The streptavidin selected constructs were amplified with PCR and purified.
GEO
GSM3399676
GEO Accession
GSM3399676