A.1
DWTR-suspended with aeration
The bacterial 16S rRNA V4-V5 fragments were amplified using forward primer 515F (5-GTGCCAGCMGCCGCGGTAA-3) and reverse primer 907R (5-CCGTCAATTCCTTTGAGTTT-3). PCR reaction was performed in the 50 L mixture that contained: 0.2 M of primer 515F, 0.2 M of primer 807R, 60 ng DNA, 25 L of 2  Premix Taq, and 20 L DNA-free water. PCR reactions were conducted with an initial denaturation at 94 C for 5 min, followed by 30 cycles of 30 s at 94 C, 30 s at 52 C, 30 s at 72 C, and a final extension at 72 C for 5 min.
A.1
SUB5972385
A.1