Bgl3_Lib3_hitemp_sort1
High-temp DMS of Bgl3 from Streptomyces,  Library3, sort1
A GH1 gene library was grown in a liquid  culture and active enzyme variants were isolated via microfluidic screening at  an elevated temperature (65 C). The plasmids for the active GH1 variants was  extracted, the GH1 gene insert was digested out of the expression vector, and  isolated via gel extraction. The GH1 gene insert was then randomly fragmented  using the Nextera XT kit and sequenced using a 2x300 MiSeq v3 kit.
Bgl3_Lib3_hitemp_sort1
SUB7240601
Bgl3_Lib3_hitemp_sort1