GSM3768316
GSM3768316: S02-LLC-NTC-GFP-3; Mus musculus; RNA-Seq
Total RNA was extracted from cells using RNeasy Plus Mini Kit (Qiagen), which includes a step to eliminate genomic DNA. RNA libraries were constructed using an Illumina TruSEQ stranded mRNA Sample Prep Kit (Cat# RS-122-2101). Total RNA was combined with RNA purification beads to bind PolyA RNA to oligodT magnetic beads. mRNA was eluted and converted to double stranded DNA. A-tailing, adapter ligation, and PCR amplification using 15 cycles was performed to complete the library construction.
GEO
GSM3768316
GEO Accession
GSM3768316