GSM4199206
GSM4199206: Wild Type Gravid Adults sRNA 20C, Rep 1; Caenorhabditis elegans; miRNA-Seq
sample collection: Whole worms were flash frozen in liquid nitrogen prior to Trizol/chloroform extraction Small RNAs (~16-30-nt) were purified from total RNA by size selection using 17% polyacrylamide gels and treated with RNA polyphosphatase to facilitate capture of 22G-RNAs. Libraries were then prepared according to the NEBNext® Multiplex Small RNA kit protocol. PCR products (~134-148 bp) were size selected using 10% polyacrylamide gels.
GEO
GSM4199206
GEO Accession
GSM4199206