E-MTAB-5954:GMR>DeIF6 Larva 3_p
Illumina HiScanSQ paired end sequencing; RNA-seq analysis of gene expression associated to altered gene dosage of DeIF6 during eye development in Drosophila melanogaster
RNA-seq analysis of gene expression associated to altered gene dosage of DeIF6 during eye development in Drosophila melanogaster
GMR>DeIF6 Larva 3_p
Fly strains were maintained on standard cornmeal food at 18°C. Flies were investigated at the third instar larva stage and in pupa 40 hours after puparium formation.  RNA was extracted with mirVana isolation kit according to the manufacturer protocols (mirVana ThermoFisher #AM 1560). Libraries for Illumina sequencing were constructed from 100 ng of total RNA. Libraries for eye imaginal disc were prepared with the Illumina TruSeq RNA Sample Preparation Kit.
INGM, National Institute of Molecular Genetics, “Romeo ed Enrica Invernizzi”, Milan, Italy
E-MTAB-5954:GMR>DeIF6 Larva 3_p
Experimental Factor: developmental stage
third instar larva stage
Experimental Factor: genotype
GMR>DeIF6
Experimental Factor: organism part
eye disc