GSM4200324
GSM4200324: Bulk_LG_Normal_120hAEL; Drosophila melanogaster; RNA-Seq
Intact LGs were collected from larvae at corredponing time points. Instead of Papain solution, Trizol (MRC, TR118) is added to LGs and performed RNA extraction. More than 1ug of total RNA were prepared for each experiment. cDNA library was constructed by 5' and 3' adapter ligation and loaded into a flow cell where fragments are captured into library adapters. Each fragment is then amplified through bridge amplification. After cluster generation finished, templates are sequenced by Illumina Trueseq.
GEO
GSM4200324
GEO Accession
GSM4200324