GSM3034480
GSM3034480: PR8 infected, hDcr-N1 transfected NoDice cells; Homo sapiens; Influenza A virus (A/hvPR8/34(H1N1)); ncRNA-Seq
Small RNA were extracted with the miRvana microRNA isolation kit (Thermo Fisher) Small RNA processed with the TruSeq Small RNA Sample Preparation Kit (Illumina). Adapter-ligated RNA was reverse transcribed using SuperScript III (Life Technologies) and amplified using GoTaq green PCR master mix (Promega) with TruSeq 3′ indices.  DNA bands corresponding to ~145 bp were isolated by gel electrophoresis on 10% polyacrylamide gels.
GEO
GSM3034480
GEO Accession
GSM3034480