Mac23
Environmental aerosol (onshore) sample taken at USA: Flushing Bay, Queens, New York City on 28-Apr-2014
The 16S universal Eubacterial primers .A single-step 30 cycle PCR using HotStarTaq Plus Master Mix Kit (Qiagen, Valencia, CA) were used under the following conditions: 94oC for 3 minutes, followed by 28 cycles of 94oC for 30 seconds;  53oC for 40 seconds and 72oC for 1 minute; after which a final elongation step at 72oC for 5 minutes was performed.  Following PCR, all amplicon products from different samples were mixed in equal concentrations and purified using Agencourt Ampure beads (Agencourt Bioscience Corporation, MA, USA).   Samples were sequenced utilizing Roche 454 FLX titanium instruments and reagents and following manufacturers guidelines
Mac23
SUB4624995
Mac23