GSM3768131
GSM3768131: SADay4(-)TGFb1_mRNA; Mus musculus; RNA-Seq
Colonies were recovered from agarose with Cel Biolab's matrix solubilization solution, RNA was extracted with Trizol, and aqueous phase was purified with Qiagen RNeasy Kit (Qiagen, Redwood City, CA, USA). Total RNA concentration and purity were assessed by the NanodropeOneC Microvolume UV-Vis Spectrophotometer (ThermoFisher Scientific, Waltham, MA, USA) with both A260/A230 and A260/A280 ratios. Library preparation was completed by Mayo Clinic's Genome Analysis Core (Rochester, MN, USA), using 100 ng -1 μg of total RNA with TruSeq RNA Sample Prep Kit v2 (Illumina Inc., San Diego, CA, USA).
GEO
GSM3768131
GEO Accession
GSM3768131