GSM2936584
GSM2936584: WT_259; Mus musculus; RNA-Seq
Small intestines were isolated using EDTA, frozen on dry ice, and RNA was harvested using Trizol reagent and purified using Omnigen RNA extraction kit. PolyA RNA was isolated using the NEBNext® Poly(A) mRNA Magnetic Isolation Module and barcoded libraries were made using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®(NEB, Ipswich MA).  Libraries were pooled and single end sequenced (1X75) on the Illumina NextSeq 500 using the High output V2 kit (Illumina Inc., San Diego CA). RNA libraries were prepared for sequencing using standard Illumina protocols
GEO
GSM2936584
GEO Accession
GSM2936584