GSM2942626
GSM2942626: Nematostella_Larva_scRNAseq; Nematostella vectensis; RNA-Seq
1. RNA extraction: cells were lysed in a water-based hypotonic buffer, containing 0.2% Triton X-100 and RNAse inhibitors. 2. polyadenylated RNAs were Reverse Transcribed, with a polyT oligonucleotide, containing a Unique Moleculer Identifier and a cell barcode. 3. cDNA was subject to In Vitro Transcription amplification, prior to adaptor ligation (that included a batch barcode) and library preparation (through 16 cycles of PCR amplification). 4. Libraries were sequenced in a NextSeq 500 machine, using a v2 mid-ouput 75 cycles kit (Illumina).
GEO
GSM2942626
GEO Accession
GSM2942626