GSM3400510
GSM3400510: Chrom-associated RNAs from ovaries of CCR4/+ rep2; Drosophila melanogaster; OTHER
small RNAs: Total RNA was isolated by Trizol (Invitrogene) and size-fractioned by PAGE (19-29 nt); chromatin bound RNA: Nuclear RNA was extractred as descirbed in (Chen et al 2016, PMID: 27292797). small RNAs: Libraries of small RNAs 19-29 nt were constructed as previously described in (Muerdter et al. 2012); chromatin bound RNAs: cDNA was synthesized using SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen). Reaction of tagmentation was performed in according to previously published protocol (Picelli et al. 2014) using in-house produced Tn5 charged with oligonucleotides compatible with the Illumina system. Reaction products were cleaned using Clean Mini (Evrogen), PCR amplified and sequenced on the Illumina HiSeq 2500.
GEO
GSM3400510
GEO Accession
GSM3400510