GSM3639334
GSM3639334: 5c-KO_H3K4me3_input; Mus musculus; ChIP-Seq
Mouse amygdala tissue was dissected from adult males. Nuclei were isolated using Nuclei EZ prep Kit (Sigma, NUC-101), and counted. 20,000 nuclei were used as input. ChIP-Seq library preparation was performed as described in PMID 25607992 with two exceptions. 1) We used a kit to generate sequencing library in one-tube reactions (NEB, E7103S). 2) We spiked in the panel of synthetic nucleosomes carrying major histone methylations (EpiCypher, SKU: 19-1001). We used the rabbit monoclonal H3K4me3 antibody (Thermo, clone #RM340).
GEO
GSM3639334
GEO Accession
GSM3639334