GSM4201003
GSM4201003: d4_3; Mus musculus; RNA-Seq
Lungs were harvested, chopped into small pieces and incubated in digestion buffer DMEM (Gibco 31966021); 0.25 mg/ml LiberaseTM TL (Research Grade 05401020001, Roche); 10mM Hepes (Gipco 1563049) for 45 mins at 37°C on Eppendorf rotator at 120rpm. Digestion was stopped with 20ml RPMI 1640 medium Thermo Fisher Scientific; 31870074) added with 50μM 2-mercaptoethonal (AppliChem, A1108). Cells were meshed through 70um cell strainer (Falcon 3522350) and washed and collected in FACS Buffer or 1× MojoSort buffer (BioLegend; 480017). Bone marrow cells from ilia, femora, and tibiae were isolated by crushing the bones in 1× MojoSort buffer. Bone marrow cells were filtered (CellTrics, 30 μm; Sysmex) and washed and collected in 1x MojoSort buffer. As described in CEL-Seq2 protocol (Hashimshony et al. 2016) Adapted from TruSeq Small RNA Library Preparation Protocol
GEO
GSM4201003
GEO Accession
GSM4201003