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RNA-Seq of Serranus cabrilla
Total RNA was extracted according to the manufacturers instructions using the total RNA isolation kit Nucleospin RNA II (Macherey-Nagel GmbH & Co. KG, Duren, Germany) and converted into first- and second-strand cDNA using respectively SuperScript III Reverse Transcriptase (Invitrogen) and Second strand cDNA synthesis (NEB) following the manufacturers instructions. Sequencing libraries were obtained using Nextera DNA XT Sample preparation kit (Illumina) and processed with MiSeq reagent kit V2 (2  250-bp paired-end [PE] mode; Illumina, San Diego, CA, USA)
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SUB5916784
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