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Clinical E.coli Strain-EC555 obtained from urinary tract infection (UTI) patients- non-treated
Approx. 250 ng of genomic DNA was sheared using Covaris S2 sonicator to generate fragent of 200-400 bp. The fragment size distribution was checked on Agilent 2200 TapeStation with D1000 DNA screen tapes and reagents and purified using HighPrep magnetic beads. The fragment was adapters ligated and then amplified for 10 cycles of PCR. The final PCR product (sequencing library) was purified with HighPrep beads, followed by library quality control check.
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SUB7241968
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