GSM3712194
GSM3712194: WT1BL; Mus musculus; RNA-Seq
Blood was extracted by cardiac suction and stored in  RNAprotect Animal Blood Tubes (Qiagen - ID: 76544). RNA qas extracted and purified using the Rneasy Protect Animal Blood Kit (Qiagen - ID: 73224). Globin mRNA was depleted using the Mouse/rat GLOBINclear Kit (Ambion - AM1981). RNA integrity and concentration were measured using a Bioanalyzer; only samples with a RIN > 7 were used. Libraries were prepared using a NEBNext Ultra Directonal RNA Library Prep Kit for Illumina (New England Biolabs - #E7760), along with the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs - #E7490S) and the NEBNext Multiplex Oligos for illumina (New England Biolabs - #E7335S).
GEO
GSM3712194
GEO Accession
GSM3712194