U1-4
RNA-Seq of A cervicornis tissue: elevated temperature
Total RNA was extracted using TRI Reagent then purified using RNeasy columns. cDNA libraries were generated using a PrimeScript First Strand cDNA synthesis kit and an additional PCR reaction to synthesize the second strand and adding barcodes, following the methods for TagSeq library prepartion by Kenkel and Matz (2016). Each cDNA library was gel extracted using a MinElute Gel Extraction kit before pooling libraries together for sequencing. Libraries were single end sequenced with a read length of 50bp on an Illumina HiSeq 2500
U1-4
SUB6041165
U1-4