T6R3-q5.IonXpress
metabarcoding of complex plant food product: tea: Sample plant_food_product_T6
"nrITS1 target fragments were amplified using Q5 Hot Start High-Fidelity 2X Master Mix (NEB, USA) with optimized primers that selectively anneal to 18S and 5.8S sequences of plants, excluding fungi. The amplified products were purified with Agencourt AMPure XP magnetic beads (Beckman Coulter, USA), then 50 ng of products were used for PCR-free library preparation. Ion Plus Fragment Library Kit and Ion Xpress Barcode Adapters Kits were used for adapter ligation. Procedures included DNA end blunting and ligation of adapters to blunt ends with no cycles of library amplification. Sequencing was carried out on the Ion S5 platform using Ion 520/530 Kit Chef reagent sample preparation kits with Ion Chef instrument and Ion 530 chips (Thermo Fisher Scientific, USA)."
T6R3-q5.IonXpress
SUB4674243
T6R3-q5.IonXpress