E-MTAB-6841:Control sample #4_s
Illumina HiSeq 2000 sequencing; RNAseq of Human Umbilical Vein Endothelial Cells treated with Insulin in the presence or absence of TGF-beta kinase inhibitor SB431542 for 90 minute.
RNAseq of Human Umbilical Vein Endothelial Cells treated with Insulin in the presence or absence of TGF-beta kinase inhibitor SB431542 for 90 minute.
Control sample #4_s
HUVECs were bought from GIBCO company HUVECs were grown in Medium 200 (ThermoFisher Catalog # M200500), 10 % Fetal Bovine Serum from HyClone company, and addition of LSGS (ThermoFisher Catalog # S-003-10) HUVECs were starved 6 hours to overnight before start of the experiment.Cell were pretreated with 5 micromolar SB431542 for 40 minutes before addition of 100 nanomolar insulin for 90 minutes. RNA was extracted using RNeasy Kit Plus Encore Complete RNA-seq DR Multiplex (Nugen Catalog # 0333-32)
Departments of Cell and Tissue Biology, and Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research
E-MTAB-6841:Control sample #4_s
Experimental Factor: stimulus
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Experimental Factor: compound
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