GSM2936265
GSM2936265: Ago2IP_1-miR146a; Mus musculus; RIP-Seq
Afte proteinase K treatment RNA was isolated using Phenol-chloroform isoamyl extraction Libraries were prepared using Clonetch SMARTer Stranded RNA-Seq Kit, starting with 8μl of the IPed RNA. The manufacturer's protocol was followed, using 4 minutes for RNA fragmentation, double SPRI bead clean-up after reverse transcription and 20 PCR cycles for amplification. The resulting libraries were sequenced using Illumina Hiseq2000 instrument with 50 base pair single read sequencing mode.
GEO
GSM2936265
GEO Accession
GSM2936265