GSM4515960
GSM4515960: Input; Homo sapiens; ChIP-Seq
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody conjugated with Gammabind G sepharose beads. Libraries from ChIPed DNA were prepared using KAPA Hyper Library Preparation Kit. Samples were end repaired, 3' ends adenylated and barcoded with multiplex adapters, followed by size selection with Ampure XP beads and PCR amplification.  Samples were next quantified by Qubit and validated on the Agilent Tapestation 4200, normalized and pooled, and then run on the Illumina NextSeq 500 using 75 cycle SBS v2.5 reagents
GEO
GSM4515960
GEO Accession
GSM4515960