GSM3955374
GSM3955374: Pax7Lo; Mus musculus; RNA-Seq
Cellular suspensions of Pax7Hi, Pax7Mi and Pax7Lo were loaded on a Chromium Controller instrument (10x Genomics) to generate single-cell gel bead-in emulsions (GEMs), respectively. GEM-reverse transcriptions (GEM-RTs) were performed in a Veriti 96-well thermal cycler (Thermo Fisher Scientific). After RT, GEMs were harvested and the cDNAs were amplified and cleaned up with the SPRIselect Reagent Kit (Beckman Coulter) Indexed sequencing libraries were constructed using the Chromium Single-Cell 30 Library Kit (10x Genomics) for enzymatic fragmentation, end-repair, A-tailing, adaptor ligation, ligation cleanup, sample index PCR, and PCR cleanup.
GEO
GSM3955374
GEO Accession
GSM3955374