GSM4368914
GSM4368914: RNA-WT-1_H5YLWDSXX_L1_1.fq.gz; Homo sapiens; RNA-Seq
For DamID-seq, genomic DNA were isolated using DNeasy Blood & Tissue Kit (Qiagen). For ChIP-seq, DNA was isolated sing phenol-chloroform-isoamylalcohol extraction and ethanol precipitation. For RNA-seq, total RNA was extracted in TRIzol (Thermo Fisher Scientific) and genomic DNA was removed using a DNA-free kit (Thermo Fisher Scientific) For ATAC-seq, library amplified and purified using TruePrep DNA Library Prep Kit V2 for Illumina(Vazyme Biotech). For DamID-seq, the DNA library was constructed using aNEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations. For ChIP-seq, the enriched fragments were constructed into libraries without the incorporation of spike-in controls via KAPA Hyper Prep Kits with PCR Library Ampli?cation/Illumina series (KK8504) following the manufacturer's instructions. For RNA-seq, sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations. DamID-seq, ATAC-seq, ChIP-seq, and RNA-seq
GEO
GSM4368914
GEO Accession
GSM4368914