H1 1.5kb mate-pair
H1 1.5kb mate-pair
DNA was used to create A “long mate-pair” DNA library as described in the “Applied Biosystems SOLiDTM 4 System Library Preparation Guide” (Applied Biosystems, Foster City, CA). One mate-pair library spanned ~1.5 kbp inserts and was made from 15 ug of DNA. The 1.5 kbp insert library was PCR-amplified 9 cycles prior to use in SOLiDTM 4 templated bead preparation. Two full slides of 2 x 50 bp sequencing was performed.
H1_gDNA_1_5kb_mp
Salk-E
H1 1.5kb mate-pair