SL242953_blood_wes
Blood whole exome sequencing: Sample SL242953
Whole-exome and RNA-sequencing on all tumor and matched normal samples were performed at the HudsonAlpha Institute for Biotechnology. DNA was extracted from germline samples and fresh frozen tissues using the Qiagen QIASymphony method. Total RNA from tumor tissues was extracted using Qiagen RNAeasy plus mini kit. DNA and RNA from all FFPE samples were extracted using the Qiagen All prep FFPE DNA/RNA kit. Each exome was captured using Roche SeqCap EZ Exome v3.0 kit and sequenced on an Illumina HiSeq 4000 platform to a depth a ~100x for germline and ~300x for tumor tissues. RNA libraries were prepared using the Illumina TruSeq RNA Exome protocol and kit reagents and further sequenced on an Illumina HiSeq 4000 with 100 million total reads per sample (50 million paired reads).
SL242953_blood_wes
NCI-phs001994
SL242953_blood_wes
alignment_software
BWA 0.7.7