H1 Illumina 500PE
500bp Paired end run on Illumina MiSeq
S. thermoautotrophicus strain H1 was isolated from soil taken from a charcoal pile in Hasselfelde, Germany.  Samples were added to mineral media and incubated in the presence of H2 and CO2 at 60C.  Pellicles emerged after several days, and were transferred and enriched on plates of mineral media plus 0.5% gellan gum and 0.075% MgCl2*6H2O in a defined atmosphere of 40% H2, 10% CO2, and 50% air, or on 45% CO, 5% CO2, and 50% air.  Genomic DNA was isolated with the DNeasy blood and tissue kit (Qiagen).  Quality was verified with a Bioanalyzer chip (Agilent).  DNA was fragmented by adaptive focused acoustics (Covaris) to 400bp or 3kb, and libraries prepared with the TruSeq or Mate Pair v2 kits (Illumina), respectively.  Libraries were sequenced with 250PE reagent kits on a MiSeq (Illumina) .  Reads were trimmed for quality with Trimmomatic26, and assembled with SPAdes 3.0
H1 Illumina 500PE
Harvard Medical School
H1 Illumina 500PE