GSM1155148
GSM1155148: T47D-overexpressing ELF5-input DNA-48hour; Homo sapiens; ChIP-Seq
Cells were washed 2 times with cold PBS and scraped into 600 μl PBS with protease inhibitors (P8340, Sigma). Fixed cells were spun for 2 minutes at 6,000xg, washed as before and snap frozen in liquid nitrogen. ChIP-Seq as previously described (doi:10.1016/j.ymeth.2009.03.001) using a 50%–50% mixture of V5-specific antibody and the Santa Cruz N20 anti ELF5 antibody, or no antibody for the input files. DNA was processed for Illumina sequencing using 36-bp reads on a GAIIx.
GEO
GSM1155148
GEO Accession
GSM1155148