GSM4200801
GSM4200801: ATAC-Ramos.2; Homo sapiens; ATAC-seq
Cells were resuspended in 50 μl of ATAC-seq RSB containing 0.1% NP40, 0.1% Tween-20, and 0.01% digitonin by pipetting up and down three  times.  This  cell  lysis  reaction  was  incubated  on  ice  for 3 min. After lysis, 1 ml of ATAC-seq RSB containing 0.1% Tween-20 (without  NP40  or  digitonin)  was  added,  and  the  tubes  were inverted to mix. Nuclei were then centrifuged for 10 min at 500 r.c.f. in a pre-chilled (4 °C) fixed-angle centrifuge. Supernatant was removed with two pipetting steps, as described before, and nuclei were  resuspended  in  50 μl  of  transposition  mix  (25 μl  2×  TD buffer (recipe in Supplementary Protocol 1) 2.5 μl transposase (100  nM  final),  16.5 μl  PBS,  0.5 μl  1%  digitonin,  0.5 μl  10% Tween-20, and 5 μl water) by pipetting up and down six times. Transposition reactions were incubated at 37 °C for 30 min in a thermomixer with shaking at 1,000 r.p.m. Reactions were cleaned up with Zymo DNA Clean and Concentrator 5 columns. All libraries were amplified with a target concentration of 20 μl at 4 nM, which is equivalent to 80 femtomoles of product. Following amplification, libraries were purified with Ampure XP beads using two-sided size selection to remove primer dimers and fragments >1000 bp.
GEO
GSM4200801
GEO Accession
GSM4200801