rin B-2
lncRNA in tomato fruit
Total RNA was extracted from the fruits of AC and rin mutant at breaker stage respectively (two biological replicates per genotype combined from 10 fruits each) using DeTRNa reagent (EarthOx, CA, USA) according to the manufacturer’s protocol. The RNA concentration and purity were measured using a NAS-99 spectrophotometer (ATCGene, NJ, USA). The RNA integrity was checked by agarose gel electrophoresis. Genomic DNA was removed from extracted total RNA by DNase treatment. Due to some lncRNAs lack ployA tail, total RNA only was treated to remove rRNA, which kept most lncRNA with ployA tail and not. The quality of the RNA and lack of contaminating ribosomal RNA were confirmed using the Agilent 2100 Bioanalyzer. Four strand-specific RNA libraries with insert size of approximately 250~500bp were prepared according to a UTP method, and submitted to BGI for 100-bp paired-end sequencing on the Illumina HiSeq 2000, at a depth of ~70 million reads per library
rin B-2
China Agricultural University
rin B-2