A5-77-2
WXS of homo sapiens: post-treatment prostate cancer R3
"Regions of tissue were excised from formalin-fixed paraffin-embedded tissues using laser capture microdissection and DNA was extracted using the QIAamp DNA FFPE Tissue Kit. 10-50ng of DNA were sheared and assembled into whole exome sequencing libraries using the SureSelect Human All Exon V7 Low Input Exome kit (Agilent). Samples were sequenced on an Illumina HiSeq 4000 with 150 cycles paired end, 8 indexing cycles and 10 molecular barcoding cycles. Pass-filter FASTQ files were trimmed using Agilent SureCall Trimmer, aligned with BWA-MEM, duplicate-marked and quality-score recalibrated using GATK 4.0.5.2."
A5-77-2
NCI-phs001938
A5-77-2
alignment_software
BWA-MEM 0.7.17