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circRNA-seq of Homo sapiens: adult male liver
Total RNA was isolated, followed by RNase-free DNase treatment to remove traces of genomic DNA. rRNA was removed from the total RNA. The rRNA-depleted RNAs were subsequently incubated with RNase R. The purified RNAs were used as templates for the construction of cDNA libraries in accordance with the protocol for the mRNA-Seq sample preparation kit. The clustering of samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumina, San Diego, USA). And the paired-end sequencing was performed on the Illumina Hiseq2500 platform (USA) following the manufacturer  s instructions.
library_06
SUB3531893
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