V3-leaf-0h-rep3-sRNA
Small RNA-Seq of Maize: Seedlings at V3 stage
Total RNA was extracted from the maize tissues using TRIzol reagent (MRC, Molecular Research Center, Inc.) according to the manufacturers instructions. A Bioanalyzer 2100 was used to check the quality of the extracted RNA, and only RNA of high integrity was used for the analysis. 20 g total RNA for each sample was run in a 15% polyacrylamide/urea gel after denaturation, and gel slices corresponding to 15- to 40-nt RNA fragments were separated. The RNA was precipitated by sodium acetate and ethanol and used for sRNA library construction with the NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB, E7300S). The pooled sRNA libraries were sequenced on the Illumina HiSeq 2500 platform at Berry Genomics (Beijing, China) to produce 50-bp single-end reads.
V3-leaf-0h-rep3-sRNA
SUB5123425
V3-leaf-0h-rep3-sRNA