GSM1154517
GSM1154517: Rrp5FLinvivo; Saccharomyces cerevisiae BY4741; RNA-Seq
Lysates were clarified and the histidine-tagged proteins were purified on nickel columns (Qiagen Ni-NTA agarose). A two-step IP was performed with one in denaturing condition (6M guanidinium). RNA-Rrp5 complexes were separated by SDS-PAGE, transferred to a nitrocelluose membrane and extracted. Protein was removed by Proteinase K digestion and RNAs purified by phenol:chloroform extraction. Extracted RNAs were converted to cDNA and PCR amplified. PCR products between ~150 and 250 bp were gel extracted and submitted for Solexa sequencing.
GEO
GSM1154517
GEO Accession
GSM1154517