GSM3333427
GSM3333427: IgG_ChIP_rep1; Arabidopsis thaliana; ChIP-Seq
Chloroplasts were isolated from 10-d-old Arabidopsis Col-0 seedlings, followed by  cross-linking with 1% (v/v) formaldehyde. Cross-linked chloroplasts pellets were suspended with IP buffer [20 mM Tris-HCl (pH 7.6), 150 mM NaCl, 1 mM EDTA, 1% (v/v) Triton X-100, 0.05% (w/v) SDS]. Then, the supernatant of chloroplast extract were sonicated. The supernatant were subsequently incubated HA antibody (abcam, ab9110) or IgG antibody (abmart, B30011)  which both conjugated to ChIP-Grade protein A/G agarose (Thermo scientific, 26161) for  4h. Finally, DNA was isolated from washed agarose beads and "Input" supernatant of chloroplast extract. DNA libraries were prepared with MGIEasy ChIP Library Prep Kit following the manufacturer's instruction.
GEO
GSM3333427
GEO Accession
GSM3333427