GSM4200463
GSM4200463: Input DNA; Mus musculus; ChIP-Seq
For ChIP-seq, the crosslinked protein-DNA complex was extracted from the sonicated nuclei and isolated with antibody. Enriched DNA for ChIP-seq, sequencing was end polished with T4 DNA polymerase and kinase. An 'A' base was added to the polished DNA fragments, followed by the Qiaquick column clean up. Sequencing adaptors were ligated to the DNA fragments and enriched by 15 cycles of PCR amplification. 200-300 bp size fractions were selectively isolated from the 1% agarose gel and eluted by the Qiagen gel extraction kit. The extracted DNA was quantified by Qubit 2.0 and subjected to HiSeq 2500 sequencing according to the manufacturer's instructions.
GEO
GSM4200463
GEO Accession
GSM4200463