GSM1154268
GSM1154268: wild-type (FGSC4200); Neurospora crassa; RNA-Seq
total RNAs of each sample were extracted and small RNAs were enriched with 5% polyethylene glycol (MW8000) and 500 mM NaCl illumina standard TruSeq small RNA preparation. For each sample, small RNAs of 18-30 nt were purified from PAGE gel. The 3' and 5' RNA adaptors were then ligated and RT-PCR were performed to amplify the library (18 cycles). Libraries were analysed with Illumina HiSeq 2000. Please see instruction of illumina Truseq small RNA preparation guide and BGI website for detailed information
GEO
GSM1154268
GEO Accession
GSM1154268