GSM3502977
GSM3502977: CL46; Homo sapiens; RNA-Seq
Total RNA was extracted from each sample using the RNeasy Mini Kit according to manufacturer's protocol (Qiagen, Inc.). The RNA concentration was measured using the Epoch microplate spectrophotometer (BioTek). Prior to library generation for RNA-sequencing, the quality of the RNA was determined on a 2200 TapeStation analyzer using RNA screen tapes (Agilent Technologies). The mRNA libraries were generated by the Biopolymers Facility at Harvard Medical School (http://genome.med.harvard.edu, Boston, MA) and included poly-A enrichment and the Directional RNASeq Wafergen services.
GEO
GSM3502977
GEO Accession
GSM3502977