GSM4453788
GSM4453788: SCABER_ATACSeq_2; Homo sapiens; ATAC-seq
Cell nuclei were isolated from 30-50K cells in lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2 and 0.1% IGEPAL CA-630). Nuclei were then incubated nuclei with Tn5 transpose (Illumina, FC-121-1030) at 37oC for 30 mins. DNA library was then purified, PCR amplified and subsequently verified for nucleosome pattern size distribution and concentration using BioAnalyzer High Sensitivity DNA Kit (Agilent). Pair-end 2x50/2x100/2x150 bp high-throughput sequencing was performed using HiSeq/NovaSeq platform (Illumina).
GEO
GSM4453788
GEO Accession
GSM4453788