PO-MiSeq
Illumina MiSeq of P. lutheri culture with oil
DNA extraction was done using PowerSoil DNA isolation kit (MO BIO) according to manufacturers instructions.  The DNA samples from each replicate experimental set were pooled to prepare the one DNA sample per each variant. An aliquot of each DNA samples was checked for quality and quantity by gel electrophoresis on the 0.8% agarose gel and by measuring the concentration of DNA by Qubit dsDNA HS kit (Life Technology) with Qubit Fluorometer. These DNA samples were used as a template for barcoded-amplicon sequencing. Extracted DNA samples were used to amplify the V4 region of 16S rRNA gene using dual-barcoded primers (Fadrosh et al. 2014). Reactions were performed in triplicate in a final volume of 25 l. PCR conditions were as follows: 94C for 45 s; 50C for 1 min; 72C for 30 s for a total of 30 cycles, followed by a final incubation at 72C for 5 min. The obtained amplicons were pooled and purified using the QIAEX II DNA Extraction kit (Qiagen).
PO-MiSeq
SUB6615396
PO-MiSeq