mM5
mop1-1 developing ear mRNA-seq, rep3
Total RNA was extracted using a mirVana miRNA Isolation Kit (Life Technologies #AM1560) with Plant RNA Isolation Aid (Life Technologies #AM9690). 5 μg  of total RNA was treated with DNase using a DNA-Free RNA Kit (Zymo Research #R1013) according to the small RNA elimination procedure. Sequencing libraries were prepared using a dUTP stranded procedure according to the Illumina guidelines (TruSeq® Stranded Total RNA Sample Preparation Guide, Part #15031048 Rev. C)
mM5
University of Georgia
mM5