GSM3503208
GSM3503208: D55_PMN_H041_T1_20180201; Neisseria gonorrhoeae; Homo sapiens; ssRNA-seq
At indicated times, samples were placed on ice. Bacteria alone were collected into microcentrifuge tubes, pelleted, and stored in RNAprotect at -80C. Supernatants were discarded from neutrophil containing samples, RNA protect was added, and samples were stored at -80C. Samples were exposed to EDTA pre-extraction and during the lysis step. Extractions were performed using Qiagen's RNeasy extraction kit as per manufacturer's protocol. An additional Turbo DNase was performed after the final step of RNeasy protocol. Quality and quantity were determined using the Agilent 4200 TapeStation System. Strand specific RNA libraries were prepared for sequencing using standard Illumina protocols
GEO
GSM3503208
GEO Accession
GSM3503208