GSM4200744
GSM4200744: S_10X01_1_het; Homo sapiens; RNA-Seq
1. 3D beta-like cell organoids were washed with PBS once.  2. Incubated with TrypLE for 1 min to dissociate the cells.  3. Diluted 5x with PBS.  4. Centrifuged at 250 rcf for 5 mins.  5. Supernatant discarded.   6. Cells resuspended in 0.04% BSA in PBS, and centrifuged at 150 rcf for 3 mins. Removed supernatant. This was repeated two more times.  7. Resuspended cells in 0.04% BSA in PBS and passed cells through a 40 μm strainer.  8. Counted cells. Ensured at least 50,000 live cells available, with at least 70% cell viability.  9. Resuspended cells at a density of 700 cells/ul. No dead cell removal was carried out. Single cells were lysed in droplets using the 10X Chromium instrument  Reverse transcription was performed in droplet and library construction was performed according to the 10X Chromium Single Cell 3' Reagent Kit User Guide
GEO
GSM4200744
GEO Accession
GSM4200744