GSM1437638
GSM1437638: GFP-only control sample 1; Mus musculus; RNA-Seq
RNA from dissociated FACS-purified GFP-expressing embryonic cortical pyramidal cells was extracted using TRIzol RNA Isolation Reagents (Life Technologies). Quality of RNA was ensured before amplification by analyzing 20–50 pg of each sample using the RNA 6000 pico kit and a bioAnalyzer (Agilent). 2.1 to 7.8 ng of high quality (RIN>8) total RNA was subsequently amplified using the SMARTer® Universal Low Input RNA Kit for Sequencing (Clonetech Laboratory) according to instructions provided by the manufacturer. The sequencing library was prepared using Kapa HTP library preparation kit (Kapa Biosystems), adaptors were diluted 1/30 fold, and amplification was set for 10 cycles. Barcoded samples were then run on a Hiseq 2500 in a 50bp/50bp paired end run, using the TruSeq SBS Kit v3 (Illumina).
GEO
GSM1437638
GEO Accession
GSM1437638