GSM4509059
GSM4509059: gRNA library2 HT29 in vivo D30 replicate1; Homo sapiens; OTHER
Genomic DNA of screening cell samples were extracted with DNA EXTRACTION Kit (Tiangen)according to the manufacturer's protocol. For gRNA library analysis, genomic DNAs from HT29, 293FT and HeLa cells for screens were extracted. gRNA library was enriched by PCR amplification. Deep sequencing of amplified PCR products containing gRNA sequences was conducted by Illumina NextSeq X ten at CAS-MPG Partner Institute for Computational Biology Omics Core, Shanghai, China.
GEO
GSM4509059
GEO Accession
GSM4509059