13VIR4541-9
A/chicken/Italy/13VIR4541-9/2013
Total RNA was purified from the 14 infected clinical samples using the Nucleospin RNA kit (Macherey–Nagel, Duren, Germany). Complete influenza A virus genomes were amplified with the SuperScript III One-Step RT-PCR system with Platinum Taq High Fidelity (Invitrogen, Carlsbad, CA) using one pair of primers complementary to the conserved elements of the influenza A virus promoter.  Sequencing libraries were obtained using Nextera DNA XT Sample preparation kit (Illumina) The indexed libraries were pooled in equimolar concentrations and sequenced in multiplex for 250 bp paired-end on Illumina MiSeq.
IZSVe
13VIR4541-9