GSM2174866
GSM2174866: 3xFlag-Ago1 WT (spy797); Schizosaccharomyces pombe; ncRNA-Seq
Nuclei were isolated and sRNA associated with Ago1 were immunoprecipitated with Flag antibody Small RNA libraries were constructed as previously described (Halic and  Moazed, 2010). Briefly, 21-24nt RNA was size-selected on a 17.5% polyacrylamide/7M  urea gel and ligated to a 3’ adapter. The ligated species were size-selected on a 17.5%  polyacrylamide/7M urea gel and ligated to a 5’ adapter. RNA was then reverse  transcribed into cDNA and PCR-amplified in a two-step process. Amplified cDNA was  gel-purified and sequenced on an Illumina High-Seq platform.
GEO
GSM2174866
GEO Accession
GSM2174866