GSM2943979
GSM2943979: Blood Neu ZT5 Replicate 1; Mus musculus; RNA-Seq
Total RNA was extracted using the Arctus Picopure RNA isolation kit (Thermo Fisher Scientific) according to the manufacturer's protocol. cDNA libraries were prepared using 2ng of total RNA and 1µL of a 1:50000 dilution of ERCC RNA Spike in Controls (Ambion) using SMARTSeq v2 protocol (Picelli et al., 2014), except for the following modifications: (1) use of 20μM TSO; and (2) use of 250pg of cDNA with 1/5 reaction of Illumina Nextera XT kit. The length distribution of the cDNA libraries was monitored using DNA High Sensitivity Reagent kit on the Perkin Elmer Labchip
GEO
GSM2943979
GEO Accession
GSM2943979