GSM1826282
GSM1826282: Total RNA extracted from AlHV-1-infected bovine lymphoblastoid cells (LCL718); Bos taurus; ncRNA-Seq
RNAs were extracted from AlHV-1-infected LCLs (10e7) of a calf developing MCF following infection with AlHV-1 infected BT cells injected intravenously. total RNA was extracted using TRI reagent (Ambion) following the manufacturer's instructions. Small RNA cloning was conducted with 30 ug of total RNA as detailed in Pfeffer et al. (2005). Nature methods 2, 269-276 and then directly sent for large-scale sequencing. Small RNA sequencing was performed at the Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Illkirch, France, using an Illumina HiSeq 2000 instrument with a read length of 36 bp. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO
GSM1826282
GEO Accession
GSM1826282