GSM4513665
GSM4513665: Knockout 2; Mus musculus; RNA-Seq
Mice were perfused with HBSS containing 2.5 mg/ml iron oxide and 0.1% BSA. At the end of perfusion, kidneys were removed, decapsulated, minced into 1-mm3 pieces, and digested in HBSS containing 1 mg/ml collagenase A and 100 U/ml deoxy- ribonuclease I. Digested tissue was then passed through a 100-mm cell strainer and collected by centrifugation. The pel- let was resuspended in 2 ml of HBSS, and glomeruli were collected using a magnet. The purity of glomerular was veri- fied under microscopy. Total RNA was isolated from kidney glomeruli of mice using the RNAeasy kit (Qiagen) RNA libraries were prepared for sequencing using standard Illumina protocols
GEO
GSM4513665
GEO Accession
GSM4513665