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16S amplicon sequencing of Mus Musculus C57BL/6: Caecal content
Library preparation was carried out by the Ramaciotti Centre, The University of New South Wales, Sydney, NSW, Australia. PCR amplification was performed with Illumina-barcoded 515F and 806R primers, following the Earth Microbiome Project workflow (Caporaso et al. 2011, Proc Natl Acad Sci USA 108:4516-22). PCR products were checked on a gel before normalisation with SequalPrep normalisation plates (Thermo Fisher Scientific, Waltham, MA, USA). Equivolumes of each sample were pooled into a library. The library was cleaned using the AxyPrep Mag PCR Clean-up kit (Axygen, Corning, NY, USA).
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SUB5390967
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