Swollen leaf bud 1 EXP
Swollen leaf bud #1 - EXP
Preparation of small RNA libraries was performed with the TruSeq Small RNA Sample Prep Kit (Illumina, San Diego, CA, USA) following manufacturer instructions. Briefly, 1 ?g of total RNA was ligated with adapters at 3’ and 5’ ends, without any size fractionation. Adapter-ligated RNA was reverse transcribed with SuperScript II Reverse Transcriptase (Invitrogen, Carlsbad, CA, USA), then PCR-amplified (15 cycles). Samples were barcoded using 15 variants of the reverse primer provided with the kit. Libraries were pooled together and then purified on a 6% TBE PAGE gel after electrophoresis. Libraries quality and concentration were evaluated with the Agilent 2100 Bioanalyzer DNA1000 assay. The obtained cDNAs were sequenced using the Illumina Genome Analyzer IIx.
Swollen leaf bud 1 LIB
CRA-GPG
Swollen leaf bud 1 EXP