GSM2942712
GSM2942712: siI1_rep1; Homo sapiens; ncRNA-Seq
RNA extraction was performed using TRIZOL according to manufacturer's protocol. 500 ng of total RNA was used in the small RNA protocol with the TruSeq™ Small RNA sample prepkit v2 (Illumina) according to the instructions of the manufacturer. The barcoded libraries were size restricted between 140 and 165bp, purified and quantified using the Library Quantification Kit - Illumina/Universal (KAPA Biosystems) according to the instructions of the manufacturer. A pool of up to 10 libraries was used for cluster generation at a concentration of 10nM using an Illumina cBot. Sequencing of 51 bp was performed with an IlluminaHighScan-SQ sequencer at the sequencing core facility of the IZKF Leipzig (Faculty of Medicine, University Leipzig)  using version 3 chemistry and flowcell according to the instructions of the manufacturer.
GEO
GSM2942712
GEO Accession
GSM2942712