9918_595431_G12_042
Sanger sequencing of HPV70 and BCL11B Homo sapiens: adult female cervix carcinoma
Total RNA (2 g) isolated from six, 10 m, tumor sections with an RNA integrity number of 9.3 (Agilent 1000) and subjected to oligoT magnetic bead enrichment.  Libraries were prepared following the NEB standard protocol as follows: cDNA was synthesized using random hexamer primers and M-MuLV RT (RNaseH-) followed by second strand synthesis with DNA polymerase I and RNaseH.  The double stranded cDNA was purified using AMPure XP beads (Beckman Coulter), end tail repaired, adaptor ligated, size-selected, and PCR amplified before Illumina sequencing. 150 bp insert cDNA libraries were sequenced to a depth of 60 million reads on one Illumina HiSeq 2500 lane (Illumina, Inc., San Diego, CA) using the paired end 150 bp mode.
9918_595431_G12_042
SUB5405682
9918_595431_G12_042