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Pseudomonas reads
A whole genome shotgun library was prepared, starting from 1ng of genomic DNA, using the Illumina Nextera XT kit. Then the library was sequenced, generating a total of 0.6M reads. All sequence reads were quality checked and assembled using SPAdes 3.6 (6). The assembly consisted of 74 contigs (N50: 242 kbp), 5607 predicted protein-coding sequences (CDSs), 65 tRNA, 1tmRNA. Compared with the reference P.koreensis D26, 71% of the assembly bases are aligned (4.5 Mbp) with OrthoANI (7) with a value of 99.94%. Genes coding for rRNA and tRNA were identified using RNammer 1.2 (8). Gene prediction were performed using Glimmer 3.2 (9). Predicted genes were annotated by automated pipeline PROKKA 1.1 (10) and against the database of Uniprot (11).
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SUB6031193
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