GSM4512076
GSM4512076: HepG2 fSHAPE vivo rep2; Homo sapiens; OTHER
RNA in fSHAPE samples was Trizol extracted and column purified. eCLIP samples were lysed (eCLIP lysis buffer), immunoprecipitated (IP samples only), Dnase treated, and column purified to select for RNA. fSHAPE libraries were contsructed from polyA selected RNA treated by NAI-N3 either in vivo or in vitro. SHAPE-eCLIP libraries were constructured from RNA immunoprecipiated by SLBP anitbody, treated with DMS/NAI in vivo or left untreated. fSHAPE-eCLIP libraries were constructed from RNA immunoprecipiated by SLBP antibody, treated with NAI in vivo or in vitro.
GEO
GSM4512076
GEO Accession
GSM4512076