E-MTAB-6723:Sample 10_s
Illumina HiSeq 2500 sequencing; RNA Seq of colonic Lgr5+ epithelial stem cells in Il22+/+ and Il22-/- mice during homeostasis and 24h after irradiation with 8 Gy
RNA Seq of colonic Lgr5+ epithelial stem cells in Il22+/+ and Il22-/- mice during homeostasis and 24h after irradiation with 8 Gy
Sample 10_s
Colonic epithelial cells were dissociated in HBSS containing 5mM EDTA and 10mM HEPES. Cells were further digested using Dispase (5 units per ml; BD Biosciences ), CollagenaseD (0.5 mg/ml; Roche) and DNaseA (0.5 mg/ml; Sigma-Aldrich). Cells were highly purified as DAPI-EpCam+CD45-CD24MedLgr5+ For γ-irradiation of mice, animals were exposed to a Caesium137 source in a OB58/905-2 irradiator (Buchler, Germany). total RNA was isolated using Trizol according to the Immgen protocol (https://www.immgen.org/Protocols/Total%20RNA%20Extraction%20with%20Trizol.pdf) SMARTer Ultra Low Input RNA (Clontech) was used for library preparation
Institute of Microbiology and Infection Immunology, Charite - University Medicine Berlin, 12203 Berlin, Germany
E-MTAB-6723:Sample 10_s
Experimental Factor: genotype
Lgr5-Cre-ERT2; Il22-/-
Experimental Factor: irradiate
gamma radiation
Experimental Factor: dose
8