pH_effects
Direct pH effects on marine bacterial communities
Amplifications were conducted in two duplicate reactions of 50 *l, each containing 20 ng of template DNA, 5 *l Taq Buffer (10x), 10 *l TaqMaster PCR Enhancer (5x), 1.25 *l of each primer (20 *M), 5 *l dNTPs (2.5 mM each), and 2.5 U of Taq DNA polymerase (5 Prime). Cycling conditions were: 94 *C for 10 min, followed by 20 cycles of 94 *C for 1 min, 44 *C for 1.5 min and 68 *C for 2 min, with a final step at 68 *C for 15 min. The duplicate reactions were combined and purified with the QIAquick PCR purification kit (Qiagen, Valencia, CA, USA). Amplicons were pooled equimolarly and sequenced on 1/2 plate on a Roche 454 GS-FLX Titanium platform.
A1133_BIOACID_Amplicons
Alfred Wegener Institute for Polar and Marine Rese
pH_effects