GSM3444348
GSM3444348: DNA AO rep 3; Homo sapiens; RNA-Seq
RNA was extracted using the MagMAX-96 Total RNA Isolation Kit, including DNase treatment (Life Technologies), according to the manufacturer's instructions. Samples were sent to the Australian Genome Research Facility (AGRF, Perth and Melbourne, Australia) for whole transcriptome library preparation using the TruSeq Stranded Total RNA Library Prep Kit (Illumina, CA, USA) and ribosomal RNA depletion with the Ribo-Zero-Gold kit (Illumina, CA, USA) Sequencing as performed using an Illumina HiSeq 2500 (Illumina, CA, USA) to generate 100 base pair single end reads, resulting in an average 25 million reads per sample. Raw sequencing files were quality checked using FastQC (0.11.7), with all files passing
GEO
GSM3444348
GEO Accession
GSM3444348