GSM4705610
GSM4705610: DGM-13434_sm (Demographics_Drop-seq); Homo sapiens; RNA-Seq
Single cell capture, RNA extraction and cDNA preparation were performed on the Drop-seq system following the manufacturer's protocol. In all cases, >80% of the cells were single cells, with 70 to 80% cell viability. Cell suspensions were filtered through a 35 µm nylon mesh cell strainer snap cap and loaded on an Influx™ cell sorter.  The single viable cells were sorted, resuspended in PBS/0.01% BSA and the sorted single cell suspension counted using Countess™ Automated Cell Counter adjusted to the final concentration of 100 to 120 cells/µl. The single cell suspension, barcoded beads, and droplet generation oil were running through a microfluidics device to form droplets.  Droplets containing both a single cell and barcoded bead were immediately lysed and the released mRNA hybridized to the primers on the surface of the beads. The single cell libraries were pooled and sequenced with paired end clustering and 101x2 cycles sequencing on the Illumina Hiseq 2500
GEO
GSM4705610
GEO Accession
GSM4705610