ISU-USDA-FAANG-AlvMac-ChIPseq-C8LPS2H-4me1
Illumina HiSeq 3000 paired end sequencing; Pig histone modification atlas
ChIP-Seq 100 cycle paired end experiment on an Illumina HiSeq 3000
4M1-P8LPS2H
NEBNext Ultra II DNA Library prep kit for Illumina
IOWA STATE UNIVERSIT
ISU-USDA-FAANG-AlvMac-ChIPseq-C8LPS2H-4me1
Iowa State Universit
ISU-USDA-FAANG-AlvMac-ChIPseq-C8LPS2H-4me1
project
FAANG
assay type
ChIP-seq
sample storage
frozen, -70 freezer
sample storage processing
cryopreservation, other
sampling to preparation interval
1
experimental protocol
https://www.neb.com/-/media/catalog/datacards-or-manuals/manuale7645.pdf
extraction protocol
http://ftp.faang.ebi.ac.uk/ftp/protocols/assays/ISU_SOP_ChIP_swine_immune_cells_20190124.pdf
library preparation location
DNA facility, Iowa State University
library preparation date
2019-05-08
sequencing location
DNA facility, Iowa State University
sequencing date
2019-05-10
experiment target
histone_modification
chip protocol
http://ftp.faang.ebi.ac.uk/ftp/protocols/assays/ISU_SOP_ChIP_swine_immune_cells_20190124.pdf
control experiment
ISU-USDA-FAANG-AlvMac-ChIPseq-C8LPS2H-input
chip target
H3K4Me1
chip antibody provider
Diagenode
chip antibody catalog
H3K4me1 (Diagenode, catalog: C15410194) H3K4me3 (Diagenode, catalog: C15410003)
chip antibody lot
A1862D
library generation max fragment size range
600
library generation min fragment size range
50