Cassava K-family
Cassava (Manihot esculenta) F1 intercross cultivars TMS30572 x CM2177-2
A full sib F1 segregating population of 132 individuals derived from single seeds of a cross between cultivars TMS30572 and CIAT’s elite cultivar CM2177-2. Total genomic DNA was extracted from young leaf tissue of 132 individuals of the F1 population and their parents TMS30572 and CM2177-2, using the commercial kit QIAGEN DNeasy Plant Mini Kit (Hilden, Germany), following the manufacturer’s protocol and adjusting the final concentration to 100 ng/μL. To assess the quality of DNA and absence of enzymatic inhibitors, a restriction digestion was performed using HindIII and visualized on a 1% agarose gel. GBS libraries were prepared and analyzed at the Institute for Genomic Diversity (IGD, Cornell University, USA), according to Elshire et al. (2011). The partial methylation sensitive ApeKI restriction enzyme that recognizes a five base pair sequence (GCWGC) was used for digestion and a library was generated with 134 unique barcodes for progeny and parents. Two lanes of Illumina Hi-seq (Illumina, Inc.) were used for the all samples.
International Center for Tropical Agriculture (CIA
Cassava K-family