het18C
RNA-Seq of nuclear and cytoplasmic RNA fractions from PRPF31+/-and wild-type hTERT-RPE1 cells
cDNA libraries were prepared using Ribo-Zero Magnetic Kit for rRNA depletion and NEBNext Ultra Directional RNA Library Prep Kit library prep kit by Novogene Inc. Library quality was assessed using a broad range DNA chip on the Agilent Bioanalyser 2100. Library concentration was assessed using Qubit and q-PCR. Libraries were pooled, and paired-end 150bp sequencing to a depth of 20M reads per fraction was performed on an Illumina HiSeq2500 by Novogene Inc.
het18C
SUB7234727
het18C