7d_seedling RIP_8pf
Ribosome-associated transcriptome sequencing from 7d old seedlings
For each in-house (7-day seedlings) RNA sample, cDNA libraries were constructed by first isolating poly-adenylated RNA (paRNA) from three *g of total or RIP RNA.  Libraries for RNA-Seq were prepared using the Illumina mRNA-Seq Sample Prep Kit. Briefly, the paRNA was fragmented and reverse transcribed using random primers. Adapters were ligated to double stranded cDNA, and fragments from 175-225 bp were gel-purified. After PCR amplification, the cDNA libraries were sequenced on an Illumina Genome Analyzer.
RIP_8pf
Michigan State University
7d_seedling RIP_8pf