GSM4087727
GSM4087727: HA-MITF-K206R_100ng_Dox_Replicate1; Homo sapiens; ChIP-Seq
30x-15cm dishes of 501mel cells were fixed in 0.4% PFA then cheared with a Covaris S220 for 15 minutes (160 W peak incident power, 5% duty cycle and 200 cycles per burst). The sonicated chromatin were precleared and ChIP was performed using 120ug of the indicated antibodies. Purification of the ChIP-DNA was done using QIAGEN PCR clean-up column Libraries were prepared using recommended Illumina protocols
GEO
GSM4087727
GEO Accession
GSM4087727