LI-039
Liver transcriptome profile in pigs with extreme phenotypes of intramuscular fatty acid composition
From the 10 selected animals, total RNA was isolated from liver using the RiboPureTM Isolation of High Quality Total RNA (Ambion*, Austin, TX) following the manufacturer’s recommendations. RNA was quantified using the NanoDrop ND-1000 spectrophotometer (NanoDrop products, Wilmington, USA) and checked for purity and integrity in a Bioanalyzer-2100 (Agilent Technologies, Inc., Santa Clara CA, USA). Sequencing libraries were generated using Illumina mRNA-Seq following manufacturer’s instructions and ten index codes were added to attribute sequences to each animal. A total of two channels of an Illumina Hi-Seq 2000 instrument (Fasteris SA, Plan-les-Ouates, Switzerland) were used to sequence two pools of five samples (one pool with five samples per lane with barcoding).
GPF-4
Facultat de Veterin*ria, Universitat Aut*noma de B
LI-039