GSM3333447
GSM3333447: R1 Pos H3K27ac Chipseq; Mus musculus; ChIP-Seq
5.10^5 (H3K27ac and H3K4me1) - 5.10^6 (H3K9ac and H3K4me3) cells per condition were cross-linked in 1% formaldehyde, lysed and sonicated as described (Tzelepis et al, 2016), using 33 cycles of 30'' ON/30''OFF. Chromatin was immunoprecipitated with anti-H3K9ac (Millipore 07-352), anti-H3K27ac (abcam -ab4729), anti-H3K4me1 (abcam ab8895) or anti-H3K4me3 (abcam ab8580) antibodies and captured with 1:1 protein A and protein G magnetic beads. After reverse cross-linking, immunoprecipitated and input DNA were purified using a Zymo DNA clean and concentrator5 kit. Standard Illumina ChIP library protocols with Nextera indices
GEO
GSM3333447
GEO Accession
GSM3333447