GSM4200727
GSM4200727: B2_E50_6d_treatment.wig; Saccharomyces cerevisiae; ChIP-Seq
Cells were lysed with 300 ml acid washed glass beads (425-600 mm) by vigorous agitation for 30 s in a BeadRuptor2 followed by 30 s on ice, repeated 7 times. Sufficient lysis (90%) was confirmed by light microscopy. The lysate was subsequently sonicated at 4°C to obtain an average DNA fragment length of 300 bp. Samples were centrifuged and the supernatant, containing sheared chromatin, recovered. Sequencing library was prepared with a NEXTflex DNA Sequencing kit (PerkinElmer) according to the manufacturer's protocol.
GEO
GSM4200727
GEO Accession
GSM4200727