E-MTAB-8726:Sample 9_p
Illumina HiSeq 2500 paired end sequencing; RNA-seq of human breast cancer cell line MCF-7 treated for 24h with rapamycin or DMSO
RNA-seq of human breast cancer cell line MCF-7 treated for 24h with rapamycin or DMSO
Sample 9_p
Samples were collected after 24h treatment with vehicle or rapamycin. Total RNAs were isolated using TRIzol reagent (Thermo Fisher Scientific) according to the manufacturer's recommendations.  Libraries were made using 1 μg of total RNA (three replicates per condition) and the TruSeq Stranded mRNA Library Preparation Kit (Illumina, Paris, France) according to manufacturer's recommendations
Universite Paris-Saclay, Institut Gustave Roussy, Inserm, Biomarqueurs predictifs et nouvelles strategies therapeutiques en oncologie
E-MTAB-8726:Sample 9_p
Experimental Factor: genotype
pLX-TRIB3
Experimental Factor: compound
dimethyl sulfoxide