GSM3874692
GSM3874692: C4_SpenDegron_Auxin28h_rep1; Mus musculus; RNA-Seq
RNA extraction was performed using the RNeasy kit and on-column DNAse digestion (Qiagen). Reverse transcription was performed on 1ug total RNA using Super Script III (Life Technologies). To quantify allelic skewing, cDNA was amplified using biotinylated primers and subsequently sequenced using Q24 Pyromark (Qiagen). Only samples showing a RIN score above 9 were used to prepare RNAseq libraries (TruSeq). Library were prepared using TruSeq 2 kit.
GEO
GSM3874692
GEO Accession
GSM3874692