BTA-DEDJTR-BBM409015438-Leg1_smallRNA-Seq
Illumina HiSeq 3000 paired end sequencing; Bovine small and micro RNA expression atlas
Small RNA sequencing of 18 tissues using HiSeq3000
BTA-DEDJTR-BBM409015438-Leg1-smRNA
NEBNext Multiplex Small RNA Library Prep kit (New England Bioloabs Inc)
DEPARTMENT OF ECONOMIC DEVELOPMENT, JOBS, TRANSPORT AND RESOURCES
BTA-DEDJTR-BBM409015438-Leg1_smallRNA-Seq
assay type
RNA-seq of non coding RNA
sample storage
frozen, -70 freezer
sample storage processing
cryopreservation in liquid nitrogen (dead tissue)
sampling to preparation interval
6
extraction protocol
https://assets.thermofisher.com/TFS-Assets/LSG/manuals/fm_1560.pdf
library preparation location
Department of Economic Development, Jobs, Transport and Resources
library preparation location longitude
144.96332
library preparation location latitude
-37.814
library preparation date
2017-01
sequencing location
Department of Economic Development, Jobs, Transport and Resources
sequencing location longitude
144.96332
sequencing location latitude
-37.814
sequencing date
2017-02-07
experiment target
CHEBI_33697
rna preparation 3' adapter ligation protocol
https://www.neb.com/-/media/catalog/datacards-or-manuals/manuale7300.pdf
rna preparation 5' adapter ligation protocol
https://www.neb.com/-/media/catalog/datacards-or-manuals/manuale7300.pdf
library generation pcr product isolation protocol
https://www.neb.com/-/media/catalog/datacards-or-manuals/manuale7300.pdf
preparation reverse transcription protocol
https://www.neb.com/-/media/catalog/datacards-or-manuals/manuale7300.pdf
library generation protocol
https://www.neb.com/-/media/catalog/datacards-or-manuals/manuale7300.pdf
read strand
mate 2 sense
rna purity - 260:280 ratio
2.02
rna purity - 260:230 ratio
0.49
rna integrity number
not applicable