ena-EXPERIMENT-CNAG-19-07-2017-14:17:20:811-1
Illumina HiSeq 2500 paired end sequencing
lib400
4.0  μ g  of  genomic  DNA  was  sheared  on  a  Covaris ™   E210  in  order  to  reach the fragment  sizes  of  ~400-800bp.  To receive a library of precise insert size (500bp - 600bp - 80 0bp) with a small size deviation the DNA  was size selected on agarose gel where the bands of 380bp, 500bp and  680bp were excised. The size  selected  DNA  was  purified  with  Qiagen  QIAquick®  Gel  Extraction  Ki t,  end-repaired,  adenylated  and  ligated to Illumina specific indexed paired-end adaptors.
CNAG
ena-EXPERIMENT-CNAG-19-07-2017-14:17:20:811-1
Centro Nacional de Análisis Genómico
ena-EXPERIMENT-CNAG-19-07-2017-14:17:20:811-1