GSM2941955
GSM2941955: WT cellular RNA rep 1; Human immunodeficiency virus 1; OTHER
Trizol extraction of RNA RNA was reverse transcribed into cDNA and a specific region of the RNA was PCRed into dsDNA. This dsDNA was then randomly fragmented before library construction for sequencing on the Illumina HiSeq2000 in 100 paired end read mode. Libraries were prepared by ligating preanneled Illumina multiplex adaptors P-GATCGGAAGAGCACACGTCT and ACACTCTTTCCCTACACGACGCTCTTCCGATCT to the blunted and A-tailed dsDNA. This was then amplified using primer PCR1.0 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT and an index containing primer CAAGCAGAAGACGGCATACGAGATNNNNNNGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT where NNNNNN is replaced with one of the Illumina TruSeq indexes
GEO
GSM2941955
GEO Accession
GSM2941955