GSM3444994
GSM3444994: TK6_DRB_DMSO_rep2; Homo sapiens; OTHER
Cells and nuclei were lysed with mild buffers. After blunting of DSBs and BLISS adapter ligation, DNA was extracted using DNA extraction buffer and proteinase K, followed by phenol/chloroform extraction and ethanol precipitation. After DNA sonication, genomic DSB ends were amplified with in vitro transcription, after which generated RNA was subjected to an Illumina small RNA-like library preparation protocol (similar to described for BLISS in Yan, Mirzazadeh et al, Nature Communications 2017).
GEO
GSM3444994
GEO Accession
GSM3444994