Needles_Bulk: Archaea
N_Bulk_Archaea
DNA samples were subject to 454 pyrotag sequencing as in (S E Dowd et al. 2008).  Pyrotag sequencing employed archaeal 16S primers Arch349F 5’-GYGCASCAGKCGMGAAW and Arch806R 5’- GGACTACVSGGGTATCTAAT (Takai & Horikoshi 2000). All samples were sequenced by a Roche 454 FLX instrument with titanium reagents following manufactures protocols (Roche Inc.). Initial generation of sequence library consisted of a one-step 25 cycle PCR with Hot Start plus Taq polymerase (Qiagen, Inc., Valencia, CA).
Needles_Bulk_Archaea
Harvard University
Needles_Bulk: Archaea