Library2
Library2
The lymphoblastoid cell line, GM06990, was obtained from Coriell Institute for Medical Research and propagated in RPMI 1640 supplemented with 50ug/ml Gentamicin and 15% serum (Fetal Clone II, Hyclone).  Cells were maintained in culture for no longer than 3 weeks, passaged every 2-3 days and maintained at a cell density of 3-8X105 cells per ml.    Cells were harvested 36-48h after passage and at a density of 5-6X105 cells / ml.  Total RNA was isolated as outlined (Chomczynski and Sacchi (1987) Anal Biochem 162(1):156-9) followed by poly A+ RNA (Kingston (1992), Current protocols in Molecular Biology. Supplement 21).  Each RNA-seq library was prepared from poly A+ RNA, which was isolated from 108 asynchronously growing cells.
Library2
University of Virginia
Library2