GSM2695931
GSM2695931: NBM_67M_SAC_CD11b_HI; Homo sapiens; RNA-Seq
FACS with  CD45+, CD163b+, CD11b+, Side-scatter low, and DAPI- directly into Qiagen RLT PLUS buffer.  RNA extraction was performed with Qiagen Rneasy PLUS Micro kit following standard operating procedures.  RNA quality was assessed using Agilent Bioanalyzer 2100. 1 nanogram of high quality (RIN>8.0) total RNA from each sample was reverse transcribed into cDNA using the Clontech SMART-Seq® v4 Ultra® Low Input RNA Kit.  Final Illumina libraries were constructed using 150pg of cDNA with the illumina Nextera XT DNA Library Preparation Kit. Low input RNA-Seq using SMARTer (SMART-seq) chemistry.
GEO
GSM2695931
GEO Accession
GSM2695931