GSM3445726
GSM3445726: del_TRF1_H3K27me3; Mus musculus; ChIP-Seq
Reverse crosslinking was achieved by addition of 20 ml of 5M NaCl and incubation at 65ºC for 8 hours. DNA was recovered by RNase and proteinase K treatment, phenol/chloroform extraction and ethanol precipitation. 10 to 15ng of DNA per sample were used for the ChIP-seq experiment. Samples were processed through subsequent enzymatic treatments of end-repair, dA-tailing, and ligation to adapters with "NEBNext Ultra II DNA Library Prep Kit for Illumina" from New England BioLabs (catalog # E7645). Adapter-ligated libraries were completed by limited-cycle PCR and extracted with a [single] double-sided SPRI size selection. Resulting average fragment size is 490 bp from which 120 bp correspond to adaptor sequences. Libraries were applied to an Illumina flow cell for cluster generation and sequenced on an Illumina NextSeq 500 sequencer, by following the manufacturer's protocols.
GEO
GSM3445726
GEO Accession
GSM3445726