GSM3333344
GSM3333344: Gall 2 - Biorep 3; Vitis riparia; RNA-Seq
Samples were dissected on ice, and flash frozen in liquid nitrogen. RNA was extracted and DNase1 treated, on column, using the Spectrum Plant Total RNA Kit (Sigma #STRN50-1KT; Sigma-Aldrich, St. Louis, MO, USA; protocol A and Appendix). The resulting RNA was further purified and concentrated with the RNeasy MinElute Cleanup Kit (Qiagen #74204; Qiagen, Hilden, Germany) and eluted with pure water. The quality of the resulting RNA was assessed using the Agilent 2100 BioAnalyzer (www.genomics.agilent.com; Agilent, Santa Clara, CA, USA), and all RNA integrity number values were found to be above 8. The Illumina libraries were constructed using the RNA TruSeq Kit (Illumina, Inc., San Diego, CA, USA), barcoded (TACT ungalled; GTAT galled), and sequenced single-end with 100bp reads on the Illumina HiSeq-2000 platform at the University of Missouri DNA core (http://dnacore.missouri.edu ).
GEO
GSM3333344
GEO Accession
GSM3333344