GSM3035175
GSM3035175: Adipocytes (ATCC) guadecitabine 1; Homo sapiens; MBD-Seq
On day 4, DNA was harvested from the adipocytes using the AllPrep kit (Qiagen) DNA from adipocytes, sonicated (180-200bp), and used as input for methylated DNA capture kit (Diagenode) according to manufacturer's protocol. This kit uses methyl-binding domain protein (MBD) to pull-down with biotin-streptavidin magnetic beads. DNA is eluted with salt buffer.  Libraries were verified using Qubit and Bioanalyzer, prior to sequencing on Illumina NextSeq. Mecap-seq libraries were prepared with NEXTflex Methyl-Seq1 kit (Bioo Scientific) according to manufacturer's protocol.
GEO
GSM3035175
GEO Accession
GSM3035175