BT224
Whole Exome Sequencing of Human Glioblastomas: Sample BT224
Prior to library preparation DNA was fragmented (Covaris ultrasonication) to 150 bp and further purified using Agentcourt AMPure XP beads. A total of 50 ng of size-selected DNA was then ligated to specific adaptors during library preparation (Illumina TruSeq). Each library was made with sample-specific barcodes, quantified using qCPR, and 2 libraries were pooled in equimolar concentrations to a total of 500 ng for whole exome enrichment using the Agilent Sure-Select all exon v2.0 hybrid capture kit. After hybrid selection, 4 to 5 capture pools were again pooled and sequenced over 4 or 5 HiSeq2000 lanes.
BT224
NCI-phs001793
BT224
alignment_software
bwa-0.7.1