Texanus_reseq_1.HA-S67-KT_BS_R1
Genotyping by sequencing of pooled domestic sunflower selected by broomrape resistance
The aim of the project was to map resistance of sunflower to a                 parasitic plant O. cumana in four inbred lines (HA-267, LIV-10, LIV-17 and AB-VL-8).                 To achieve this, we sequenced 8 bulk DNA samples originating from four different F2                 populations that were developed as follows: HA-267 was crossed with OD-DI-82, LIV-10                 and LIV-17 were crossed with HA-26-PR, and AB-VL-8 was crossed with L-OS-1. All of                 these lines are proprietary inbred lines developed at Insitute of Field and                 Vegetable Crops, Serbia. Segregating F2 populations with 150, 106, 89 and 96                 individuals, respectively, were obtained by self-pollination of individual F1. The                 F2 plants were advanced to produce F3 families which were phenotyped to broomrape                 resistance. After phenotyping of the F3 families, we used the selective bulked                 segregant analyses coupled with genotyping-by-sequencing to genotype the four                 mapping populations. Within each mapping population, F2 plants that produced F3                 families with no segregation for resistance or susceptibility were selected and                 contrasting DNA bulks were prepared. At least 10 plants were included in each bulk.                 The GBS libraries were prepared following the two enzymes protocol. Fragments were                 ligated to adaptors including the Illumina sequencing adaptor and size selection was                 performed to retain amplified fragments of length between 300-500bp. To reduce the                 representation of repetitive sequences in the libraries, we performed a depletion                 step by treating the enriched libraries with Duplex-Specific Nuclease. Pair-end                 sequencing 125bp was performed on an Illumina HiSeq v4. This data is currently                 unpublished. These data aim to map resistance loci involved in sunflower resistance                 parasitic plan O.cumana.
Texanus_reseq_1.HA-S67-KT_BS_R1
SUB4401855
Texanus_reseq_1.HA-S67-KT_BS_R1