ena-EXPERIMENT-CNAG-20-07-2017-12:22:20:815-5
Illumina HiSeq 2000 paired end sequencing
324Q
500 ng of  total  RNA  were  used  as  the  input  material  and  enriched  for  the  m RNA  fraction  using  oligo-dT  magnetic  beads.  The  mRNA  was  fragmented  in  the  presence  of  divalent  metal   cations  and  at  high  temperature  (resulting  RNA  fragment  size  was  80- 250   nt,  with  the  major  peak  at  130nt).  After  first  and  second  strand  cDNA  synthesis, the  double  stranded  cDNA  was  end- repaired,  3 ́adenylated,  and  thereafter  ligated  to  the  Illumina  barcoded  adapters. After ligation,  the  product  was enriched  by  15  cycles of PCR.
CNAG
ena-EXPERIMENT-CNAG-20-07-2017-12:22:20:815-5
Centro Nacional de Análisis Genómico
ena-EXPERIMENT-CNAG-20-07-2017-12:22:20:815-5