GSM2174877
GSM2174877: BJATAClib3; Homo sapiens; OTHER
Simultaneously lysed and transposed with 0.01% w/v digitonin in ATAC-seq transposition reaction. Libraries were constructed by transposition of permeabilized cells with Illumina Nextera Tn5 transposase that inserts short sequencing adapters.  The transposed DNA was purified using a Qiagen MinElute PCR cleanup kit and overhangs were extended by 5 minute incubation at 72˚C, followed by PCR with barcoding primers to introduce the remaining adapter sequence. i7 barcodes were used for multiplexing (TAAGGCGA, CGTACTAG, AGGCAGAA, TCCTGAGC, GGACTCCT, TAGGCATG, CGAGGCTG, AAGAGGCA).
GEO
GSM2174877
GEO Accession
GSM2174877