GSM2175914
GSM2175914: WST_2nd_6w; Rattus norvegicus; RNA-Seq
For RNA extraction, we used Trizol kit by following manufacturer's instructions Trizol-isolated RNA was enriched for Poly(A+) RNA with biotinylated oligo(dT), converted to cDNA with Superscript III (Invitrogen). Next, a terminal transferase (NEB, cat.no.M0315S) was used to add a ddNTP to the 3’-end of the cDNA. After the second-strand cDNA synthesis, 23 cycles of PCR were performed to amplify the cDNA. The length of 200-400nt PCR product was subjected to deep sequencing.
GEO
GSM2175914
GEO Accession
GSM2175914