GSM3712563
GSM3712563: shYTHDF2-MeRIP-rep2; Homo sapiens; RIP-Seq
The intact mRNA isolated from total RNA samples was chemically fragmented into 100-nucleotide-long fragments by incubation in fragmentation buffer, and then was enriched with m6A antibody RNA-seq libraries of m6A antibody enriched mRNAs and input mRNAs were prepared using KAPA Stranded mRNA-seq Kit (Illumina) as the following steps: 1) first strand cDNA synthesis; 2) second strand cDNA synthesis; 3) A-tailing; 4) Adapter ligation; 5) PCR amplification; 6) Library purification. QC of the completed libraries was performed with Agilent 2100 Bioanalyzer.
GEO
GSM3712563
GEO Accession
GSM3712563