GSM3186863
GSM3186863: No_21_pig_1623_IZ; Sus scrofa; RNA-Seq
Cardiac samples (50-80 mg/sample, n= 3 sham and 3 MI animals per condition) from anterior left ventricular wall of sham animals and of infarct zone and border zone of MI animal hearts were collected for RNA studies. Samples were cut into small pieces and immersed in RNAlater solution (Ambion, USA) for 48 hrs at 4°C. Then, tissue samples were homogenized and total RNA was isolated using RNAzol (Sigma-Aldrich, USA) and further purified by Purelink RNA kit (Ambion, USA). RNA-seq libraries were prepared as previously described (PMID 29160304). Briefly, RNA was quantified using a Qubit RNA High-Sensitivity Assay kit (Life Technologies, USA) and assessed for degradation on the basis of their RNA integrity number using the LabChip GX microfluidics platform and RNA assay reagent kit (Perkin Elmer, USA). TruSeq Stranded mRNA Library Prep kit (Illumina, USA) was used or library preparation and libraries quantified using KAPA library quantification kits (KAPA Biosystems, USA) according to the manufacturer's protocol. The quality and average fragment size of the final libraries were determined using a LabChip GX DNA High Sensitivity Reagent Kit (Perkin Elmer, USA). Libraries were sequenced on an Illumina NextSeq sequencer using 76bp Paired End chemistry.
GEO
GSM3186863
GEO Accession
GSM3186863