GSM1826002
GSM1826002: 43F; Oryza rufipogon; RNA-Seq
Total RNA from each sample was isolated with TRIzol (Invitrogen) following the manufacturer's instructions and then treated with RNase-free DNase I for 30 min at 37°C (New England BioLabs) to remove residual DNA. For Illumina's digital gene expression (DGE) system, the mRNAs were separately isolated with oligo(dT) ligated beads and reverse transcribed into double-stranded cDNA. The cDNAs were digested by the restriction enzymes NlaIII and MmeI. After ligation with sequencing adapters, the molecules were amplified by PCR.  For RNA-seq technology, beads with oligo(dT) were used to isolate poly(A) mRNA. First-strand cDNA was synthesized using random hexamer primers and reverse transcriptase (Invitrogen). The second-strand cDNA was synthesized using RNaseH (Invitrogen) and DNA polymerase I (New England BioLabs). The paired-end cDNA libraries with insert sizes of approximately 200 bp were then prepared according to Illumina's protocols.
GEO
GSM1826002
GEO Accession
GSM1826002