De Novo Mutation Cell 23
De Novo Germline Mutation by Whole-Genome Single Sperm Sequencing
Single sperm whole-genome amplification was performed using a microfluidic system. Amplified DNA was sheared by Covaris S2 into 200 bp, followed by NuGEN Encore NGS Multiplex System I library construction. A 4-bp barcode is added at the beginning of Read 1 for multiplex sequencing.
P0 Sperm Multi #5
Stanford University
De Novo Mutation Cell 23