GSM3954982
GSM3954982: SUM149-EMP2 knockdown # 2; Homo sapiens; RNA-Seq
RNA was extracted using Qiagen total RNA purification as per the manufacturer's instructions. Total RNA was quantified and 260/280 ratios determined using Nanodrop (all samples with a 260/280>1.97). 1μg of total RNA was used as starting material for each sample. RNA sequencing and library preparation was performed by the UCLA Technology Center for Genomics and Bioinformatics with Kapa hyper Prep Kits with RiboErase. The workflow consists of rRNA depletion, cDNA generation, and end repair to generate blunt ends, A-tailing, adaptor ligation and PCR amplification. Different adaptors were used for multiplexing samples in one lane. Sequencing was performed on Illumina Hiseq for a single read 50 run.
GEO
GSM3954982
GEO Accession
GSM3954982