GSM1155709
GSM1155709: RBPJ in mitosis rep2; Mus musculus; ChIP-Seq
After wash steps, cells were resuspended in lysis buffer and proceeded to the sonication with 40% power amplitude, 30sec, 12 times total. Cell debris was removed by 10 min centrifuge, 15,000 rpm at 4℃. Extracted chromatin supernatant was used for the following chromatin immunoprecipitation or frozen in -80℃ for storage. To generate the library from ChIP experiemtn, ChIP-Seq Library Protocol with multiplexing was used. This protocol was developed before Illumina released its multiplex truSeq ChIP-seq kit, and the details can be found on http://ngsc.med.upenn.edu/. According to the protocol, 10 ng of ChIP DNA product was used to start the library construction.
GEO
GSM1155709
GEO Accession
GSM1155709