S9-1_Q5
metabarcoding of complex plant food product: spice
The two-step PCR method was used for library preparation, in a way similar to protocol in work of [Speranskaya et al., 2018], but with new optimized primers that selectively anneal to 18S and 5.8S sequences of plants, excluding fungi. Q5 Hot Start High-Fidelity 2X Master Mix (NEB, USA) was used for nrITS1 amplification. Illumina libraries were sequenced on the MiSeq with MiSeq Reagent Kit v2 for 500 cycles with 251 + 251 cycles setting.
S9-1_Q5
SUB4674243
S9-1_Q5