GTCCGC_CACGAT
V4 region of eukaryotic rRNA gene
300bp paired-end sequencing of  the library was performed with the Illumina MiSeq 2500 platform with NEXTflex  DNA sequencing kits and an identifying NEXTflex DNA barcode with 8-base  indices .Two different PCR from the same cDNA: alpha and beta. Run 2.
GTCCGC_CACGAT
SUB5228492
GTCCGC_CACGAT