Lane 1 GBS MAGIC population
GBS data of a MAGIC population in common bean. Lane C30YAACXX_7
A common bean Multi Parental Advanced Generation Inter Cross population (MAGIC) was developed from 8 parental CIAT breeding lines (SXB 412, INB 827, ALB 213, SEN 56, SRC 2, MIB 778, SCR 9, INB 841), selected to combine desirable characteristics such as tolerance to heat stress, yield under drought and growth under unfavorable soil conditions such as high Aluminum or low Phosphorous stress. Genotypes were combined by pairwise crosses and subsequently crosses of F1 plants. After the F2 population lines were advanced by single seed decent to F5, when DNA was extracted from young leaves. Total genomic DNA was extracted from 25-days-old seedlings plants. 1 gr of leaf tissue was frozen using liquid nitrogen and ground to a fine powder with metal balls in a paint shaker for three minutes.  The DNA was extracted with the Urea based DNA extraction midi prep protocol of Chen et al. (1992). The quantity of the DNA was measured with NanoDrop 1000 and the DNA integrity was visualized trough EcoRI, PstI and RsaI restriction enzyme digestion in a 1\% agarose gel electrophoresis. DNA was sent to the Cornell sequencing facility, where GBS libraries were generated based on the Cornell protocol using ApeKI as restriction enzyme.
International Center for Tropical Agriculture (CIA
Lane 1 GBS MAGIC population