GSM1436942
GSM1436942: N8_ESC_1; Mus musculus; RNA-Seq
Total RNA was extracted using TRIzol (Invitrogen) followed by on-column DNase treatment with RNase-free DNase and RNesay mini kit (Qiagen). 2μg total RNA was used to make one sequencing library. Two biological replicates were made for each condition: WT ESC, REST-/- ESC, WT NP, and REST-/- NP. Indexed libraries were prepared using the Illumina TruSeq RNA Sample Preparation Kit v2 (San Diego, CA). Four libraries of ESC or NP were mixed at equal concentration and sequenced by an Illumina HiSeq 2000 sequencer at the OHSU Massively Parallel Sequencing Shared Resource (MPSSR).
GEO
GSM1436942
GEO Accession
GSM1436942