Z_Stress3
RNA-Seq of yeast
Eukaryotic mRNA was enriched by magnetic beads with Oligo (dT) by binding A-T to the ployA tail of the mRNA. Subsequently, break the mRNA into short fragments. The mRNA was used as a template to synthesize a strand of cDNA with six random bases and then the buffer, dNTPs and DNA polymerase I were used to synthesize the double-stranded cDNA. AMPure XP beads were then added to purify double-stranded cDNA. PCR-enrichment was performed to obtain the final cDNA library.
Z_Stress3
SUB3765315
Z_Stress3