GSM3260251
GSM3260251: Microdissected Rat Renal cTAL, Lithium_72h, RNA-Seq, Transcriptome 05; Rattus norvegicus; RNA-Seq
After microdissecting cortical collecting duct and cortical collecting duct,  Total RNA were extracted using the Direct-zol™ RNA Kits (Zymo Research) and cDNA was synthesized by SMARTer V4 Ultra Low RNA kit (Clontech). The cDNA was then amplified for 15 PCR cycles, purified by AmPure XP magnetic beads (Beckman Coulter Genomics), and eluted with 15 μL Elution Buffer. The purified cDNA was further quantified by Qubit. Library size distribution was determined using the Agilent 2100 bioanalyzer using High-Sensitive DNA kit. The Nextera XT DNA Library Preparation Kits (Illumina) was used to generate the libraries with input of 1 ng. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO
GSM3260251
GEO Accession
GSM3260251