GSM1437522
GSM1437522: MG-1655_Urine_rep3; Escherichia coli str. K-12 substr. MG1655; RNA-Seq
To stop bacterial growth, ice was added to the MOPS and urine-grown samples and the cultures were harvested by centrifugation (10 min, 8,000g, 4°C). The supernatant was discarded and 500µl of saline (0.9% NaCl) was added to each sample. Samples were then treated with RNA Protect Bacterial Reagent (QIAGEN) to stabilize RNA according to the manufacturer’s instructions RNA-seq libraries were prepared using the TruSeq RNA Sample Prep Kits (Illumina). High RNA quality was confirmed prior to library construction using Agillent’s TapeStation 2200. 50 bp-long single end reads were then sequenced on an Illumina Hi-Seq 2500
GEO
GSM1437522
GEO Accession
GSM1437522