GSM2357626
GSM2357626: CLIP_AGO2_LET7_3; Mus musculus; RIP-Seq
RNA was recovered from the nitrocellulose using Proteinase K treatment followed by phenol–chloroform extraction and ethanol precipitation. Isolated RNA were subjected to small RNA libraries preparation (New England Biolabs), following the manufacturer’s instructions. cDNA products were then subjected to PCR amplification for 20–24 cycles and size-fractionated on columns. PCR products representing cDNA inserts of 20–50 nts were recovered and subject to sequencing on a Ion Proton™ System for Next-Generation Sequencing
GEO
GSM2357626
GEO Accession
GSM2357626