GSM4368685
GSM4368685: RPF (30-40 nts) control; Escherichia coli; OTHER
Lysates were then loaded onto 10-55% sucrose gradients (gradient buffer: 100 mM NH4Cl, 10 mM MgCl2, 20 mM Tris pH 8.0 and 100 µg/ml choramphenicol) and centrifuged at 35,000 rpm for 2.5 hr at 4˚ C. Monosomes fractions were collected and ribosome-protected mRNAs were isolated by acid phenol extraction and isopropanol precipitation. Following dephosphorylation, RNAs (20 µg each) were fractionated on 15% TBE-urea gels and the appropriate size ranges, 30-40 nt or 45-150 nt, were excised. RNAs were eluted from the gel slices.
GEO
GSM4368685
GEO Accession
GSM4368685