GSM3576619
GSM3576619: Setlow_2018_08_27_S3; Bacillus subtilis; RNA-Seq
RNA was extracted and purified from spores using Ambion RiboPure-Bacteria Kit (product # AM1925). RNA quality was analyzed on the Agilent TapeStation 4200 (Agilent Technologies, Santa Clara, CA, USA) using the RNA High Sensitivity assay.  Only samples with Ribosomal Integrity Numbers (RINe) values above 7.0 were considered for library preparation RNA libraries were prepared for sequencing using Illumina TruSeq Stranded mRNA Sample Preparation kit. Libraries were validated for length and adapter dimer removal using the Agilent TapeStation 4200 D1000 High Sensitivity assay, and then quantified and normalized using the dsDNA High Sensitivity Assay for Qubit 3.0 (Life Technologies, Carlsbad, CA, USA). Illumina MiSeq v3 chemistry (paired end 2 x 75bp read length).  Sequencing read depth was targeted at 10-15 million total paired end reads/sample.
GEO
GSM3576619
GEO Accession
GSM3576619