NT22.2 Archaea-July 2008-2
NT22.2 Archaea-July 2008-2
The 16S rRNA V6 hypervariable region of archaeal DNA was PCR amplified using barcoded primers. The forward primer sequence was 5’-GCCTTGCCAGCCCGCTCAG-X-AATTGGANTCAACGCCGG-3’, where the first segment comprises the 454 primer A adaptor, X denotes a unique 10-nucleotide barcode sequence for each sample, followed by the 16S archaea 958arcF primer sequence. The reverse primer sequences were 5’- GCCTTGCCAGCCCGCTCAG-CGRCGGCCATGCACCWC-3’ (4B-R16SAMAJOR) and 5’- GCCTTGCCAGCCCGCTCAG-CGRCRGCCATGYACCWC-3’ (4B-R16SAMINOR), where the first segment comprises the 454 adaptor and the second segment comprises each archaeal reverse primer sequence.  Barcoded samples were purified and mixed. Purified PCR products were sequenced at the Australian Genome Research Facility, Brisbane, Australia.  Sequence data were separated by barcode into individual samples.
NT22.2 Archaea-July 2008-2
Charles Darwin University
NT22.2 Archaea-July 2008-2