GSM2694724
GSM2694724: YCCZ5; Cordyceps militaris; RNA-Seq
Total RNA of mycelium was extracted using RNeasy Plant Mini kit and the residual genomic DNA was digested using RNase-free DNase I according to the manufacturer’s protocol. Poly(A) mRNA was enriched by beads with oligo (dT) and interrupted by fragmentation buffer into suitableshort fragment.100 ng purified and enriched mRNA per sample were used to construct the respective cDNA libraries using NEBNext® UltraTM RNA Library Prep Kit for Illumina (NEB,USA). cDNA fragments of 200 bps (±25 bps) were selected and purified by gel-electrophoresis as the templatesfor amplification with PCRfor end reparation andadding poly (A). Six cDNA library would be accomplished after been connectedwith sequencing adaptors
GEO
GSM2694724
GEO Accession
GSM2694724