Lib_FUSCCTNBC158.TT_WES
Whole exome sequencing of Homo sapiens: triple negative breast cancer from FUSCCTNBC project
Qualified genomic DNA from tissues and matched white blood cell samples was prepared for WES. A total of 300 ng of each DNA sample based on Qubit quantification was fragmented on a Bioruptor Plus sonication system (Diagenode, Lige, Belgium). Sheared DNA was used to perform end repair, A-tailing and adapter ligation with an Agilent SureSelectXT Library Prep Kit (Agilent Technologies, Santa Clara, CA, USA) according to the manufacturers protocol. Then, 750 ng of prepared DNA in a volume of 3.4 l was captured using Agilent SureSelect Human All Exon V6 (Agilent Technologies) probes, followed by the amplification of the captured library with indexing primers
Lib_FUSCCTNBC158.TT_WES
SUB4403732
Lib_FUSCCTNBC158.TT_WES