GSM4293849
GSM4293849: WT_PD1_Hi_LTi_rep1; Mus musculus; RNA-Seq
Single cell suspension were obtained from the colon lamina propria of C57BL/6 mice. For sorting huPD-1high LTi and huPD-1- LTi cells, cells were stained with relative antibodies followed by sorting on a BD Aria III cell sorter for  "live Lineage-CD127+KLRG-1-c-Kit+CCR6+huPD-1high" and "live Lineage-CD127+KLRG-1-c-Kit+CCR6+huPD-1-" cells. The sorted LTi were collected in lysis buffer of the SMART-Seq HT Kit (Takara) followed with cDNA synthesis and amplification according to the manufacturer's protocols. Sequencing libraries were generated using NEB Next Ultra DNA Prep Kit (New England Biolabs), according to the manufacturer's instruction. Libraries were assessed using the Agilent 2100 Bioanalyzer system and sequenced by paired-end 150 base pair reads using the Illumina NovaSeq platform.
GEO
GSM4293849
GEO Accession
GSM4293849