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RNA-Seq of ALV-J infected roosters
The designed DNA probe is hybridized with the RNA samples to remove the rRNA from the total RNA; RNA is fragmented by incubating at a high temperature in the presence of magnesium before carrying out 1st strand cDNA synthesis, and the second strand cDNA is synthesized by the chain-specific method with the dUTP, at the same time, the end repair is completed. After the steps of adding the A-tail, the ligation link, the ligation product purification, the fragment size sorting, and the library amplification, RNA-Seq library was obtained. The quality and size distribution of the amplified libraries were confirmed by electrophoresis on Agilent 2100 Bioanalyzer microfluidic chips. Libraries were quantified using the KAPA Library Quantification Kit (KK4824, Kapa Biosystems). The libraries were pooled at equimolar concentrations and loaded onto the HiSeq X10 PE150 sequencing system (Illumina).
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SUB7028024
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