GSM3034489
GSM3034489: RNA-Muscle-Innerv-dKO-rep2; Mus musculus; RNA-Seq
To extract total RNA, 500 µl of TRI Reagent (Sigma) was added to frozen tibialis anterior and homogenized with PreCellys (Bertin Technologies) (6500 RPM, 3 × 10 s) in tube containing ceramics beads (Lysing Matrix D – MP biomedicals). After centrifugation, TRI Reagent was removed and beads were washed one time with 500 µl of TRI reagent. Total RNA was then extracted following provider's instruction. Libraries of template molecules suitable for strand-specific high-throughput DNA sequencing were created using a TruSeq Stranded Total RNA with Ribo-Zero Gold Prep Kit (RS-122-2301; Illumina)
GEO
GSM3034489
GEO Accession
GSM3034489