GSM3503224
GSM3503224: WT_4H_37C; Shigella flexneri; RNA-Seq
Immediately following incubation, 400 µL of culture was transferred into 800 µL of RNAprotect Bacterial Reagent (Qiagen, #76506), and treated as indicated by the supplier. The total RNA was then extracted using the RNeasy Mini Kit as indicated by the supplier (Qiagen, #74104). Libraries were generated using the KAPA Stranded RNA-Seq Library Preparation Kit (Kapa Biosystems), as per the manufacturer's recommendations. TruSeq adapters and PCR primers were purchased from IDT. Libraries were quantified using the Quant-iT™ PicoGreen® dsDNA Assay Kit (Life Technologies) and the Kapa Illumina GA with Revised Primers-SYBR Fast Universal kit (Kapa Biosystems). Average size fragment was determined using a LabChip GX (PerkinElmer) instrument.
GEO
GSM3503224
GEO Accession
GSM3503224