GSM2936608
GSM2936608: DKO-TUMOR2; Mus musculus; RNA-Seq
A piece of small intestines were collected, frozen on dry ice, and RNA was harvested using Trizol reagent and purified using Omnigen RNA extraction kit. PolyA RNA was isolated using the NEBNext® Poly(A) mRNA Magnetic Isolation Module and barcoded libraries were made using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®(NEB, Ipswich MA).  Libraries were pooled and single end sequenced (1X75) on the Illumina NextSeq 500 using the High output V2 kit (Illumina Inc., San Diego CA). RNA libraries were prepared for sequencing using standard Illumina protocols
GEO
GSM2936608
GEO Accession
GSM2936608