MG10
ITS Fungal Diversity of  Sorghum Seed
Six commercial cultivars of sorghum were obtained from the seed company Pannar (South Africa). Seed samples, which were pre-treated with fungicides. Twenty seeds of each of the six cultivars were randomly collected from the bag, and surface sterilized. The seeds were freeze dried in 50 ml Falcon tubes, ground, bashed with 2-mm-diameter metal beads in a Qiagen TissueLyser II cell disrupter (Whitehead Scientific, Cape Town, South Africa) and the powder transferred to 2 ml Eppendorf tubes for DNA extraction. DNA was extracted using the Nucleospin Plant II mini Kit (Macherey Nagel, Germany). The ITS2 region of the ribosomal operon was amplified using the primers ITS3F  and ITS4R , with overhang Illumina adapters. The amplicons were sent for sequencing library construction and sequencing at the Next Generation Sequencing Facility at the University of the Free State, South Africa on an Illumina MiSeq platform.
MG10
SUB4400667
MG10