GSM2175002
GSM2175002: O5; Trametes versicolor; RNA-Seq
Total RNA was isolated separately from T. versicolor with RNA extraction Kit RNA samples were first treated with RNAse-free DNase I to remove contaminatinged DNA. Then, the mRNA wais enriched by using the oligo(dT) magnetic beads. After mixed with theadding fragmentation buffer, the mRNA was fragmented into short fragments (about 200 bp). NextThen, the first strand of cDNA was synthesized by using random hexamer-primers. Buffer, dNTPs, RNase H, and DNA polymerase I were added to synthesize the second cDNA strand. The double- stranded cDNA was then purified usingwith magnetic beads. End reparation and 3’-end single nucleotide A (adenine) addition was then performed. Finally, sequencing adaptors were ligated to the fragments. The fragments were enriched by PCR amplification. During the QC step, an Agilent 2100 Bioanaylzer (Agilent Technologies, Germany) and ABI StepOnePlus Real-Time PCR System (Applied Biosystems, USA) were used to perform qualitfy-control steps  and to quantify the sample library. The library products wasere then ready for sequencing using anvia Illumina HiSeqTM 2000 (GATC Biotech, Germany), or another sequencer when if necessary
GEO
GSM2175002
GEO Accession
GSM2175002