H17_gc_tfh
Bulk T cell receptor beta chain sequencing within HIV infected human lymph node: Sample S8
TCR beta chain mRNA were reverse transcribed using gene-specific primers ligated to a 12N molecular identifier and a sequencing adaptor. 1st PCR was carried out using the adaptor sequence and forward primers against the variable regions. 2nd PCR was performed to add the full illumina sequencing adaptors. 2nd PCR products were gel purified and quantified via Agilent Tapestation before pooling for sequencing.
H17_gc_tfh
UT-phs001548
H17_gc_tfh