GSM1436098
GSM1436098: Del Baf60c rep2 D10; Mus musculus; RNA-Seq
Total RNA was isolated from the samples using miRNeasy micro kit (Qiagen, cat no. 217084) following manufacture protocol with on-column DNase I digestion. Total RNA was qunatified using nanodrop and quality checked using bioanalyser. RNA-seq libraries were prepared with ovation RNA-seq system v2 kit (NuGEN). The double-stranded DNA was then amplified using single primer isothermal amplification (SPIA). Random hexamers were used to amplify the second-strand cDNA linearly.  Finally, libraries from the SPIA amplified cDNA were made using the Ultralow DR library kit (NuGEN). The RNA-seq libraries were analyzed by Bioanalyzer and quantified by qPCR (KAPA). All 12 indexed samples were sequenced in each of three lanes on an Illumina HiSeq 2500.
GEO
GSM1436098
GEO Accession
GSM1436098