GSM4142996
GSM4142996: siBTF3_1; Homo sapiens; RNA-Seq
RNAs were isolated from PC-3 cells using the TRIzol (Invitrogen) reagent by following the company manual. RNA degradation and contamination was monitored on 1% agarose gels.  RNA purity was checked using the NanoPhotometer® spectrophotometer  (IMPLEN, CA, USA) .  RNA concentration was measured using Qubit® RNA Assay Kit in Qubit®2.0  Flurometer (Life Technologies, CA, USA).  RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer  2100 system (Agilent Technologies, CA, USA). Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations.
GEO
GSM4142996
GEO Accession
GSM4142996