GSM4200339
GSM4200339: null_H3K27ac; Mus musculus; ChIP-Seq
pkd1 and null cells were cross-linked with 1% formaldehyde for 10 min at RT. The crosslink was stopped by adding 1/20 volume of 2.5 M glycine. Cells were washed with PBS and harvested using ChIP lysis buffer. Cells were then sonicated to obtain fragments (100-500 bp) with Bioruptor Sonicator. Immunoprecipitation was performed with H3K27ac antibody (Abcam cat# ab4729). After elution and reversal cross-linking, DNA was purified and sequenced on BGISEQ-500. Construction of ChIP-seq library was completed by BGI company (Shenzhen,China).
GEO
GSM4200339
GEO Accession
GSM4200339