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Genome-wide identification and characterization of cucumber microRNAs in response to Pseudoperonospora cubensis infection- Control
Cucumber was grown in a greenhouse at 16 h day/8 h night, 22?-28?. At the age of 6 weeks, plants were inoculated by an inoculation solution containing P. cubensis conidia (2x 106 spores ml–1), 5 mM glucose, and 2.5 mM KH2PO4. The inoculation solution was applied on both leaf surfaces using a soft brush. The 7 dpi leaves of P. cubensis infection (Ps7d) and control (CK) were sent to Meiji (Shanghai, China) for small RNA deep sequencing.
Zhejiang Sci-Tech University
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