GSM4367932
GSM4367932: MouseTMS2; Mus musculus; RNA-Seq
Tissues were dissected in Ames' medium, digested in papain, and manually triturated into single cell suspensions in ovomucoid solution. The cells were resuspended in 0.04% non-acetylated BSA in Ames to solutions of ~1000 cells/uL and loaded into Chromium Single Cell Chips for a targeted recovery of 6,000 cells. Single cell libraries were made with Chromium 3' v3 platform (10X Genomics, Pleasanton, CA) following the manufacturer's protocol. Briefly, single cells were partitioned into Gel bead in EMulsion (GEMs) in the GemCode instrument with cell lysis and barcoded reverse transcription of RNA, followed by amplification, shearing and 5' adaptor and sample index attachment. On average, approximately 10,000 single cells were loaded on each channel and approximately 6,000 cells were recovered. Libraries were sequenced on Illumina HiSeq 2500.
GEO
GSM4367932
GEO Accession
GSM4367932