PM1-BR1
RNA-seq of Talaromyces marneffei PM1, SDA, 37C°C->25°C for 6hrs, replicate 1
At the beginning of the experiment, conidia of strain PM1 were inoculated in SDA plates and cultured at 37°C for a week. The germinated cells were transferred into new SDA plates every week for 2 weeks to establish stable colonies in yeast growth form. One week before the extraction of total RNA, in order to obtain fresh cells, the homogenous cells were cultured in new SDA plates. Yeast growth plates were transferred to 25°C for 6 hours before extraction. The total RNAs were extracted using the E.Z.N.A. fungal RNA kit (Omega Bio-Tek), following the manufacturer’s instructions with DNase I digestion to eliminate genomic DNA.
BR1
Texas A&M University
PM1-BR1