Noncoding RNAs repressed by Ino80
Strand-specific RNA sequencing of Ino80 mutant budding yeast- tet-ino80, replicate 1
Strand-specific RNAseq libraries created using the dUTP method. Strain was grown in YC(-His) with doxycycline added to 20ug/ml at 37 degrees C until OD600 ~0.4-0.7.  Total RNA was isolated using acid phenol extraction.  rRNA was depleted and libraries were creating using TruSeq.  MATa his3-1 leu2-0 met15-0 ura3-0 tetR(HIS3)-INO80-FLAG
tet-ino80
Fred Hutchinson Cancer Research Center
Noncoding RNAs repressed by Ino80