GSM4512017
GSM4512017: WT_Ly6C-Mo_rep1; Mus musculus; RNA-Seq
Total RNA was prepared using an RNeasy Micro Kit (QIAGEN) from approximately 3,000 to 10,000 cells according to the manufactures' instructions. cDNA was synthesized and amplified using a SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech). Amplified cDNA was purified using Ampure XP beads (Beckman Coulter) to remove primer dimers. RNA-seq libraries were prepared using a Nextera XT Kit (Illumina). The prepared libraries were sequenced on a NextSeq 500 (Illumina) to generate single-end 75 nucleotide reads.
GEO
GSM4512017
GEO Accession
GSM4512017