GSM4368929
GSM4368929: dPPRrbcL rep2; Arabidopsis thaliana; RNA-Seq
Isolation of chloroplasts and stroma extracts as well as immunprecipitation protocol see: Jiang,J., Chai,X., Manavski,N., Williams-Carrier,R., He,B., Brachmann,A., Ji,D., Ouyang,M., Liu,Y., Barkan,A., et al. (2019) An RNA Chaperone-Like Protein Plays Critical Roles in Chloroplast mRNA Stability and Translation in Arabidopsis and Maize. Plant Cell, 31, 1308–1327. Recovered RNA was further purified with Monarch® RNA Cleanup Kit (NEB) and 50 ng of each RNA were used for libraries generation with the NEBNext® Ultra™ II RNA Library Prep Kit according to manufacturer's instructions.
GEO
GSM4368929
GEO Accession
GSM4368929