GSM4511861
GSM4511861: d+56kb_LMPP_rep2_H3K27ac; Mus musculus; ChIP-Seq
Sorted cells were fixed with 1% formaldehyde for 10 min at room temperature and the reaction was quenched with 125 mM glycine. The lysates were sonicated using a Diagenode Bioruptor-Pico sonication device (12 cycles of 30 seconds on and 30 seconds off). An aliquot of sheared chromatin was immunoprecipitated with anti-H3K27ac antibody (MABI0309, Cosmo Bio) using Dynabeads M-280 sheep anti-mouse immunoglobulin G (IgG) (Thermo Fisher Scientific) or anti-IRF8 antibody (C-19, Santa Cruz) using Dynabeads protein G (Thermo Fisher Scientific) at 4˚C. Then, ChIP-seq libraries were prepared with a TruSeq ChIP sample prep Kit (Illumina).
GEO
GSM4511861
GEO Accession
GSM4511861