GSM3444809
GSM3444809: chip_input_eFTreg; Mus musculus; ChIP-Seq
Cells were chemically cross linked by 1% formaldehyde. Cell lysates were made by appropriate sonication and protein-DNA complexes were isolated with indicated antibodies (anti-H3K27Ac: AbCam, ab729, anti-Foxp3: AbCam, ab150743) Libraries were prepared according to Illumina's instructions. Briefly, DNA was end-repaired and the blunt-ended, then phosphorylated ends were treated with Taq polymerase and dATP to yield a protruding 3- 'A' base for ligation of NEBNext adapters which have a single 'T' base overhang at the 3' end. After adapter ligation, DNA was PCR amplified with NEBNext index primers for 15 cycles and library fragments of ~250 bp (insert plus adaptor and PCR primer sequences) were band isolated from an agarose gel, and quantitated by Qubit (Invitrogen).
GEO
GSM3444809
GEO Accession
GSM3444809