GSM3502264
GSM3502264: Nelfb_KO_L30; Mus musculus; OTHER
Nuclei of cells from each condition were isolated and subjected to Run-on experiment with biotin-CTP and -UTP supplement to label nascent RNA. RNA was isolated and hydrolyzed. Then two biotin enrichment processes were preformed with interval of adding -OH group in 3' end and removing 5' cap of enriched RNA fragments. Enriched biotin-labeled RNA were used for further library construction. RNA libraries were prepared by using NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB, cat.no.E7300). After quality control, libraries were subjected to sequencing with standard Illumina protocols. PRO-Seq
GEO
GSM3502264
GEO Accession
GSM3502264