GSM3446101
GSM3446101: PVV3.7: JEG-3 control 3; Homo sapiens; RNA-Seq
RNA from each sample was isolated using RNeasy Mini Kit (Qiagen N. V., Netherlands) following manufacturer´s recommendations and eluted in 30 µl nuclease free water. RNA was purified using RNase-Free DNase Set (Qiagen N. V., Netherlands). RNA libraries were prepared for sequencing using the kits NEBNext® Poly(A) mRNA Magnetic Isolation Module (Cat# E7490) and NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (Cat#E7770), following manufacturer´s instructions and 8-11 cycles of amplification.
GEO
GSM3446101
GEO Accession
GSM3446101