GSM4511842
GSM4511842: WT_LT-HSC_rep2 [ATAC-Seq]; Mus musculus; ATAC-seq
5,000 to 7,200 cells were directly sorted into 1.5-mL tubes and pelleted by centrifugation for 10 minutes at 300g. The cells were then incubated with transposase solution (25 µL of 2 x Tagment DNA buffer and 2.5µL of Tagment DNA Enzyme [Illumina Nextera DNA Library Prep Kit], 0.5 µL of 1% digitonin [Promega], and 22 µL of distilled water) at 37˚C for 30 minutes with shaking at 300 rpm. Transposed DNA was then purified using a MinElute Reaction Cleanup Kit (QIAGEN). Purified transposed DNA was amplified using a NEBNext High Fidelity 2 x PCR Master Mix (New England Biolabs) with indexed primers. Libraries were purified using SPRIselect beads (Beckman Coulter) to remove primer dimers.
GEO
GSM4511842
GEO Accession
GSM4511842