GSM3326078
GSM3326078: RBMX_RIP_IP_Rep3; Homo sapiens; RIP-Seq
Cell extracts were resuspended in NT2 buffer [50mM Tris HCl pH=7.5, 150mM NaCl, 1mM MgCl2, 0.05% NP40, 1 U/ml Ribolock (Fermentas, Glen Burnie, MD, USA), 2mM DTT, 30mM EDTA supplemented with a protease inhibitor cocktail (P8340, Sigma, RNA libraries were prepared for sequencing using standard Illumina protocols: the Illumina TruSeq Targeted RNA Custom Panel Kit was used with 500 ng of total or polysomal RNA.
GEO
GSM3326078
GEO Accession
GSM3326078