GSM2696142
GSM2696142: cd41_GFPlow.P3.D05; Danio rerio; RNA-Seq
Zebrafish cells are isolated from the kidney marrow or thymus and resuspended in single cell suspension. Individual cells are sorted by fluorescence-activated cell sorting into 96-well plates containing 5 ul TCL buffer (QIAGEN) + 1% BME (Bio-Rad). Plates were snap frozen and stored in -80°C before reverse transcription and library construction. Whole-transcriptome amplification and library construction were performed on single cells using a modified Smart-seq2 protocol adapted by Venteicher et al. (PMID 28360267). Libraries of uniquely barcoded cells were combined and sequenced with a Nextseq 500 High Output V2 kit (75 cycles) (Illumina) on a NextSeq 500 platform (Illumina).
GEO
GSM2696142
GEO Accession
GSM2696142