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WES homo sapiens: male PB
We will use the Agilent SureSelect XT Human All Exon [v.4 51Mb] kit for exome capture and Illumina HiSeq 2000 for sequencing. For each sample, we will construct the fragment libraries using 3 ug of genomic DNA as starting material following the Agilent standard library preparation protocol for Illumina TruSeq. We will hybridize a total of 500 ng of captured library with the baits for 48 hours, then wash and elute using the Agilent protocol. After quantification with Agilent Tape Station and Life Technology Qubit we will sequence the amplified captured libraries using the Illumina HiSeq 2000 instrument and standard protocols. We will follow standard Illumina TruSeq multiplexing (barcoding) protocol to sequence multiple samples in one lane. We aim to generate the targeted number of reads for each sample, and cover more than 85% of the targeted regions by 15 reads and 90% covered by 10 reads.
wp283
SUB5405579
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