GSM4367433
GSM4367433: HfadR; Escherichia coli BL21(DE3); RNA-Seq
Total RNA was extracted using the RNAprep pure Cell/Bacteria Kit (Tiangen) following lysozyme treatment. RNA degradation and contamination were monitored on 1 % agarose gels. RNA concentration was measured by Qubit® RNA Assay Kit in Qubit® 2.0 Flurometer (Life Technologies, CA, USA) and RNA integrity was assessed on a 2100 Bioanalyzer (Agilent Technologies, CA, USA). rRNA is removed using a Ribo-zero kit that leaves the mRNA. A total of 3 μg RNA was used as an input per sample. Sequencing libraries were generated using a NEBNext®UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer's instructions, and index codes were added to attribute sequences to each sample. Clustering of the index-coded samples was performed on a cBot Cluster Generation System using the TruSeq PE Cluster Kit v3-cBot-HS (Illumina) according to the manufacturer's instructions.
GEO
GSM4367433
GEO Accession
GSM4367433