GSM4200384
GSM4200384: input.expon.chip-seq.r2; Saccharolobus solfataricus; ChIP-Seq
Cells were lysed in lysis buffer (50 mM HEPES/NaOH pH 7.5, 140 mM NaCl, 1 mM EDTA, 0.1% Na-deoxycholate, 1% Triton X-100) and DNA was sheared by sonication. Lysates were cleared by centrifugation. Chromatin-immunoprecipitation was carried out with protein-A-purified polyclonal antibodies. Immunoprecipitated DNA was recovered by QIAquick PCR purification NEBnext Ultra II DNA library preparation
GEO
GSM4200384
GEO Accession
GSM4200384