11 shVSIG1
C11 shVSIG1
Single  cell RNA capture and library preparation using 10x Genomics solution 3 kit  V2.        3500-4000 Cells were loaded into the Chromium Cell A Chip and GEM was  generated using the chromium Controller. GEM-RT was performed on thermocycler  (Biometra T3000) and stored overnight.    Post RT cleanup was performed using Silane DynaBeads and Size selection was  performed using SPRIslect. PCR amplification was performed on Biometra TOne  and the number of cycles were    were adapted according to the protocol.  the number of cycles were 13 for cDNA amplification and/ 8 cycles for  Library index.    1st QC (post cDNA amplification) was performed on 2100 bioanalyser using a  High Sensitivity DNA Kit, we quantified the fragment from 200bp to  9000bp.    2nd QC (post library construction) was performed on 4200 Tape Station using  a High Sensitivity D1000 ScreenTape, we quantified the fragment from 300bp to  900bp.
11 shVSIG1
SUB7077948
11 shVSIG1