GSM2935040
GSM2935040: TRMT2A_HAP1_iCLIP_rep_2; Homo sapiens; RIP-Seq
iCLIP was performed as previously described using a UV-crosslinking based method (Konig et al. 2012). The TRMT2A antibody used for immunoprecipitation was as for FICC-Seq. Libraries containing in-line barcodes were sequenced on the Illumina HiSeq4000 platform. Cells were harvested by washing in Phosphate Buffered Saline (PBS) and then incubating with Trypsin-EDTA followed by further washing of detached cells in PBS.
GEO
GSM2935040
GEO Accession
GSM2935040