GSM1825699
GSM1825699: MCF7_PMA_FAIRE_rep1; Homo sapiens; FAIRE-seq
5x10^6 cells were cross-linked with 1% formaldehyde for 10 minutes, the reaction is then quenched by adding 2.5M glycine (final concentration of 125mM). Fixed cells were lysed in 500ul SDS lysis buffer and chromatin bound DNA was then sheared by sonication. Nucleosome free DNA (FAIRE DNA) was extracted by using phenol-chloroform and reverse-crosslinked at 65oC. The Zymo-SpinTM I kit (Zymo Research) was then used to purify FAIRE DNA. FAIRE DNA (20ng), and the corresponding total input, were used to prepare the FAIRE-seq DNA library using the NEBNext® Ultra DNA Library Prep Kit for Illumina® (New England BioLabs)
GEO
GSM1825699
GEO Accession
GSM1825699