GSM1435572
GSM1435572: plus-minus-3; Mus musculus; RNA-Seq
Total RNA was extracted using the RNeasy Mini Kit (Qiagen) including on-column DNase digestion. Poly-A RNA was isolated from total RNA using the NEBNext Poly(A)mRNA Magnetic Isolation Module (E7490). Libraries for the RNA-seq samples were constructed using the NEBNext Library Prep Kit (6100) or NEBNext Ultra RNA Library Prep Kit for Illumina (E7530) following the instruction manual. Briefly, poly-A RNA was fragmented to generate 200 nucleotides long fragments. First and second strand cDNA synthesis was performed and the resulting cDNA was end-repaired, ligated to NEBNext adaptors. The cDNA was size selected using agarose gels or Agencourt AMPure XP Beads (Beckman Coulter) and amplified by PCR. For the resulting RNA-seq libraries, amplicon sizes and quantities were determined using the Biorad Experion system. Libraries were sequenced on an Illumina Genome Analyzer IIx following the manufacture’s instructions.
GEO
GSM1435572
GEO Accession
GSM1435572