GSM1826438
GSM1826438: p65_ChIPSeq_IL1_1h_rep1; Homo sapiens; ChIP-Seq
For ChIP-seq: Cells were crosslinked with 2 mM disuccinimidyl glutarate (DSG) for 45 min and then 1% formaldehyde for 15 min at room temperature. Following cell lysis, chromatin was sheared using a Misonix 3000 probe sonicator (30 x 30 sec, 30 sec off). p65-bound chromatin was then immunoprecipitated using 8 micrograms anti-p65 (sc-372/C-20X) antibody. Following washing and reverse crosslinking, chromatin was purified using phenol-chloroform extraction. For RNA-seq: total mRNA was isolated using RNeasy Kit (Qiagen). DNA and mRNA libraries were created following standard Illumina protocols with PolyA capture for mRNA. ChIP-seq and RNA-seq; 2-lane flow cell (single-end 50 bp sequencing)
GEO
GSM1826438
GEO Accession
GSM1826438