BTA-DEDJTR-6819-Lung2-RNA-Seq
Illumina HiSeq 3000 paired end sequencing; Bovine gene expression atlas
Paired end RNA sequencing of bovine tissues using polyA selection on an Illumina HiSeq3000
BTA-DEDJTR-6819-Lung2-RNA
SureSelect Strand Specific RNA Library Prep Kit
DEPARTMENT OF ECONOMIC DEVELOPMENT, JOBS, TRANSPORT AND RESOURCES
BTA-DEDJTR-6819-Lung2-RNA-Seq
assay type
RNA-seq of coding RNA
sample storage
frozen, -70 freezer
sample storage processing
cryopreservation in liquid nitrogen (dead tissue)
sampling to preparation interval
20
extraction protocol
https://assets.thermofisher.com/TFS-Assets/LSG/manuals/Trizol_Plus_man.pdf
library preparation location
Department of Economic Development, Jobs, Transport and Resources
library preparation location longitude
144.96332
library preparation location latitude
-37.814
library preparation date
2016-10
sequencing location
Department of Economic Development, Jobs, Transport and Resources
sequencing location longitude
144.96332
sequencing location latitude
-37.814
sequencing date
2016-11
experiment target
CHEBI_33697
rna preparation 3' adapter ligation protocol
https://www.agilent.com/cs/library/usermanuals/public/G9691-90010.pdf
rna preparation 5' adapter ligation protocol
https://www.agilent.com/cs/library/usermanuals/public/G9691-90010.pdf
library generation pcr product isolation protocol
https://www.agilent.com/cs/library/usermanuals/public/G9691-90010.pdf
preparation reverse transcription protocol
https://www.agilent.com/cs/library/usermanuals/public/G9691-90010.pdf
library generation protocol
https://www.agilent.com/cs/library/usermanuals/public/G9691-90010.pdf
read strand
mate 1 sense
rna purity - 260:280 ratio
2.12
rna purity - 260:230 ratio
2.11
rna integrity number
7.1