wPip_Istanbul_RsR
Illumina sequencing of wPipIV_Istanbul Wolbachia strain endosymbiont of Culex pipiens
Wolbachia being intracellular uncultivable bacteria, a protocol inspired from Ellegaard et al was developed to obtain satisfying quantity of Wolbachia DNA. To do so, Wolbachia enrichment was performed on egg-rafts right after oviposition to maximize the ratio between Wolbachia genomes and Culex genomes. After severals filtrations of a crushed egg-rafts solution to concentrate Wolbachia and removed cell debris, nuclear and mitochondrial genome, a Multiple-displacement amplification (MDA) was carried out directly using the Wolbachia pellet as a matrix, with help of a Repli-g- mini kit (Qiagen)
wPip_Istanbul_RsR
SUB2844735
wPip_Istanbul_RsR