GSM3502871
GSM3502871: Gad_rpl22_B1; Mus musculus; RNA-Seq
Centrifuge the brain tissue sample to prepare supernatant and mix with Qiazol aliquot. This is total RNA sample usied for immunoprecipitation. Ribosome-associated mRNA libraries were prepared using TruSeq® Stranded Total RNA Library Prep (Illumina # RS-122-2201) following the manufacturer protocol. In brief, 0.5ug of total RNA was treated with Ribo-zero rRNA removal and cleaned with RNACleanXP. Both cDNA strands were synthesized, Adenylation of 3' end was done and indexing adapters were ligated. Libraries were amplified and the final product was purified using Agencourt AMPure XP (Beckman Coulter, #A63881). Libraries were validated using High sensitivity DNA bioanalyzer (Agilent).
GEO
GSM3502871
GEO Accession
GSM3502871