GSM3186795
GSM3186795: RW1 cultured with succinate replicate 1; Sphingomonas wittichii; RNA-Seq
Total RNA was isolated from early-to-mid log phase cultures (CFU of 1.0x109 to 1.5x109 cells/mL) using PureLink Mini Kit (Life Technologies, Carlsbad, CA) with lysis step modified as per the RiboPure Kit instructions (Life Technologies, Carlsbad, CA). To ensure complete removal of DNA, RNA samples underwent a two-step DNAse I treatment, first with DNAse I (amplification grade, Life Technologies) followed by TURBO DNAse I (Life Technologies). RNA concentration was measured using Qubit (Life Technologies) and its quality was validated using BioAnalyzer (Agilent Technologies, Santa Clara, CA). RNA libraries were prepared for sequencing using standard Illumina protocols
GEO
GSM3186795
GEO Accession
GSM3186795