GSM3576748
GSM3576748: S3_N_S61_normal_miRNA; Homo sapiens; miRNA-Seq
Total RNA was extracted using Trizol reagent (15596026, Invitrogen). All libraries were prepared using the Illumina TruSeq Small RNA protocol with minor modification following the manufacturer's instructions with 12 cycles of PCR amplification after ligation of the 3' and 5' adapters (5' TCTGGAATTCTCGGGTGCCAAGGAACTCC). Individual libraries were prepared using a unique index primer in order to allow for pooling of multiple samples prior to sequencing. The library was quantified using qPCR. Sequencing was performed on a Hiseq2500 (Illumina Inc., San Diego, CA) and image processing and base calling were conducted using Illumina's pipeline.
GEO
GSM3576748
GEO Accession
GSM3576748