Cellulolytic Streptomyces RNAseq
Differential Expression of Plant Biomass Degrading Genes in Streptomyces species
RNA analysis. RNA was purified from Streptomyces strains using a modified phenol-chloroform extraction protocol, DNase treatment, and ribosomal RNA removal (Chen and Weimer 2001, Takasuka, Book et al. 2013, Book, Lewin et al. 2014). cDNA library construction and single-end 100 bp Illumina HiSeq2000 sequencing was performed at the University of Wisconsin Biotechnology Center (Madison, WI). Data were demultiplexed using the CASAVA software package, and analysis was perfomed using a previously published pipeline (Book, Lewin et al. 2014)).    Book, A. J., G. R. Lewin, B. R. McDonald, T. E. Takasuka, D. T. Doering, A. S. Adams, J. A. Blodgett, J. Clardy, K. F. Raffa, B. G. Fox and C. R. Currie (2014). "Cellulolytic Streptomyces strains associated with herbivorous insects share a phylogenetically linked capacity to degrade lignocellulose." Appl. Environ. Microbiol. 80(15): 4692-4701.  Chen, J. Q. and P. J. Weimer (2001). "Competition among three predominant ruminal cellulolytic bacteria in the absence or presence of non-cellulolytic bacteria." Microbiol. U.K. 147: 21-30.  Takasuka, T. E., A. J. Book, G. R. Lewin, C. R. Currie and B. G. Fox (2013). "Aerobic deconstruction of cellulosic biomass by an insect-associated Streptomyces." Sci. Rep. 3.
Streptomyces sp. SirexAA-E RNA
University of Wisconsin - Madison
Cellulolytic Streptomyces RNAseq