DMX5
Replicate 3 RNA-seq for mus musculus:  adult male DBA/2J quadricep muscle
cDNA library for RNA-Seq was  generated from 100ng-400ng total RNA using TruSeq RNA Sample Preparation Kit  (Illumina, San Diego, CA, USA) according to the manufacturers protocol.  Briefly poly-A tailed RNA molecules were pulled down with poly-T oligo attached  magnetic beads. Following purification, mRNA was fragmented with divalent  cations at 85C, and then cDNA was generated by random primers and SuperScript  II enzyme (Life Technologies). Second strand synthesis was performed followed  by end repair, single `A` base addition and ligation of barcode indexed  adaptors to the DNA fragments. Adapter specific PCRs were performed to  generate sequencing libraries. Libraries were size selected with E-Gel EX 2%  agarose gels (Life Technologies) and purified by QIAquick Gel Extraction Kit  (Qiagen). Libraries were sequenced on HiSeq 2500 instrument.
DMX5
SUB5992975
DMX5