E-MTAB-5954:GMR/+ Pupa 3_p
Illumina HiSeq 2500 paired end sequencing; RNA-seq analysis of gene expression associated to altered gene dosage of DeIF6 during eye development in Drosophila melanogaster
RNA-seq analysis of gene expression associated to altered gene dosage of DeIF6 during eye development in Drosophila melanogaster
GMR/+ Pupa 3_p
Fly strains were maintained on standard cornmeal food at 18°C. Flies were investigated at the third instar larva stage and in pupa 40 hours after puparium formation.  RNA was extracted with mirVana isolation kit according to the manufacturer protocols (mirVana ThermoFisher #AM 1560). Libraries for Illumina sequencing were constructed from 100 ng of total RNA. Libraries for pupal retinae were prepared with random primed cDNA protocol according to "GATC biotech" company protocols.
INGM, National Institute of Molecular Genetics, “Romeo ed Enrica Invernizzi”, Milan, Italy
E-MTAB-5954:GMR/+ Pupa 3_p
Experimental Factor: developmental stage
pupa
Experimental Factor: genotype
GMR/+
Experimental Factor: organism part
retina