GSM4200159
GSM4200159: Hmga2-/- Ctrl; Mus musculus; RNA-Seq
Total RNA was isolated using Trizol (Invitrogen). RNA was treated with DNase (DNase-Free DNase Set, Qiagen) and repurified using the miRNeasy micro plus Kit (Qiagen). Total RNA was quantified using a Nanodrop Spectrophotometer (ThermoFisher Scientific, Germany). Total RNA and library integrity were verified on LabChip Gx Touch 24 (Perkin Elmer). 1µg of total RNA was used as input for SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Clontech). Sequencing was performed on the NextSeq500 instrument (Illumina) using v2 chemistry with 1x75bp single end setup.
GEO
GSM4200159
GEO Accession
GSM4200159