GSM3500537
GSM3500537: KPC adipose 5; Mus musculus; RNA-Seq
RNA extraction and purification from tissue snap frozen in liquid nitrogen was done via homogenization in Trizol and the Qiagen miRNeasy Mini Kit (skeletal muscle) and the Qiagen RNeasy Lipid Tissue Mini Kit (adipose tissue). Adipose: The concentration and quality of total RNA samples was first assessed using Agilent 2100 Bioanalyzer. A RIN (RNA Integrity Number) of five or higher was required to pass the quality control. Then 100 nanograms of RNA (muscle) or 500 nanograms of RNA (adipose) per sample were used to prepared dual-indexed strand-specific cDNA library using TruSeq RNA Access Library Prep Kit (Illumina). The resulting libraries were assessed for its quantity and size distribution using Qubit and Agilent 2100 Bioanalyzer. Two hundred pico molar pooled libraries were utilized per flowcell for clustering amplification on cBot using HiSeq 3000/4000 PE Cluster Kit and sequenced with 2×75bp paired-end configuration on HiSeq4000 (Illumina) using HiSeq 3000/4000 PE SBS Kit. A Phred quality score (Q score) was used to measure the quality of sequencing. Greater than 95% (muscle) or >90% (adipose) of the sequencing reads reached Q30 (99.9% base call accuracy).
GEO
GSM3500537
GEO Accession
GSM3500537