GSM4142362
GSM4142362: SAC2; Staphylococcus aureus; RNA-Seq
Extracting RNA from cultured bacteria under different conditions (presence and absence of BITC). A total amount of 3 μg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumia) according to the manufacturer's instructions. After cluster generation, the library preparations were sequenced on an Illumina Hiseq platform and paired-end reads were generated. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO
GSM4142362
GEO Accession
GSM4142362