rer2_sample_1
WGS of S. cerevisiae: delta rer2 mutant_mix_1
For each of the five genes, we select 30 largely independent clones which the focal essential gene has been deleted and their corresponding parental strain in the yTHC stock. So we sequenced the genomes of the 30 * 5 + 5= 155 clones. Three genomes were pooled together and we had 55(10×5+5) samples for the five essential genes. Fragments between 400bp and 600bp were collected for library construction and samples were sequenced on an Illumina Hi-seq 2000 platform. Approximately 8 million 125bp reads were generated for each sample, corresponding to an average sequencing depth of ~80. The gene_sample_0 represent the parental strain from yTHC stock.
Sun Yat-sen University
rer2_sample_1