thb14
Artificial selection of the Ral-GTPases enhancing Actin-based Mortality in Racing Horse
Genomic DNA quality check (DNA QC) was carried out with agarose gel   fluorescence-based quantification check with standard run electrophoresis of 0.6% agarose gel and Pulse Field gel Loading 200ng. Briefly, pair library construction (500bp fragment) followed the manufacturer’s manuscript; Purified genomic DNA, fragments of less than 800bp, blunt-ended fragments with 5’-phosphorylated ends, 3’-dA overhang, adaptor-modified ends, purified ligation product, genomic DNA library. Then, sequence data were generated using Illumina’s HiSeq.
thoroughbred
Seoul National University
thb14