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16srDNA-seq of cecal content: control mice on AIN-93M diet
Total bacterial DNA was isolated from frozen cecal contents using a QIAamp DNA Stool Mini Kit (Qiagen, Valencia, CA). The region V3-V4 of the 16S rRNA gene was amplified and DNA libraries were constructed according to previously described . The paired-end sequencing with a read length of 2250 bp was performed on the Illumina MiSeq platform.
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SUB3925007
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