GSM3639185
GSM3639185: WT control replicate 3 20190223; Arabidopsis thaliana; RNA-Seq
Total RNA was extracted using Qiagen RNeasy Plant Mini Kit according to the manufacturer's protocol.  RNA was quantified using a NanoDrop spectrophotometer and quality was monitored with an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA) and run on 2% agarose gel with post-run staining. TruSeq Stranded Total RNA Library Prep Kit; input 3 ug total RNA, poly-A RNA were purified by oligo-dT beads; USER digest treatment was done to the adaptor ligation product; final library was amplified by 10 cycles of PCR using KAPA HiFi Mastermix. Library concentrations were normalized using KAPA Library Quantification Kit for Illumina. Stranded-specific RNA-seq with oligo-dT purification.
GEO
GSM3639185
GEO Accession
GSM3639185