Experiment1
Bacterial pool 1
Bacteria were grown until exponential phase in LB broth. DNA was isolated from the bacteria with the DNeasy kit (QIAGEN) following the instructions of the manufacturer. Yield of double-stranded DNA was quantified with the Qubit spectrofluorimeter (Invitrogen). DNA from eight bacteria were mixed with human background DNA in the following proportions: Staphylococcus epidermidis 2.44 ng, Proteus mirabilis 24.39 ng, Bacillus subtilis 24.39 ng, Klebsiella pneumoniae 50 ng, Aeromonas hydrophila 24.39 ng, Pseudomonas putida 24.39 ng, Pandoraea apista 50 ng, and Burkholderia cepacia 50 ng.
Experiment1
SUB1545693
Experiment1