Trophozoite_H3K9me3
H3K9me3 ChIP-Seq of Plasmodium falciparum: trophozoite
Synchronized parasites were extracted in 0.15% saponin and crosslinked for 10 min with 1% formaldehyde at 37C. Chromatin was sheared on a Covaris S220 for 6 min (5% duty cycle, 140 intensity peak incident power, 200 cycles per burst) and diluted 1:1 in ChIP buffer. ChIP was performed O/N using 2 ug of antibody (or no antibody for control).
Trophozoite_H3K9me3
SUB2027979
Trophozoite_H3K9me3