2018_nanopore_nmosc
Ion torrent sequencing of Nitrospira moscoviensis: bioreactor  sample
For Nanopore sequencing DNA Library  construction the Ligation Sequencing Kit 1D (SQK-LSK108) in combination with  the Native barcoding Expansion Kit (EXP-NBD103) was used following the  manufacturers protocol using 3 g of gDNA as input. Shearing of the DNA was  performed using the Covaris g-TUBE (Covaris Inc., Woburn, MA, USA) at 5,000  rpm for 60 s according to manufacturers protocol. After shearing the  fragments a DNA repair treatment was performed using the NEBNext FFPE DNA  Repair Mix (New England Biolabs, Ipswich, MA, USA) followed by a purification  step. End repair and dA-tailing was done using the NEBNext Ultra II End  Repair/dA-Tailing Module (New England Biolabs), followed by a purification  step. Selected barcodes for each sample were ligated using the Blunt/TA  Ligase Master Mix (New England Biolabs) and again followed by a purification  step. Thereafter adapters were ligated using the NEBNext Quick Ligation  Module (New England Biolabs). The final library was purified and quantified  and then sequenced on the MinION sequencing device (Oxford Nanopore  Technologies, Oxford, UK) according to the manufacturers protocol.
2018_nanopore_nmosc
SUB4878554
2018_nanopore_nmosc