GSM3188282
GSM3188282: MB-24h-AR_3; Drosophila melanogaster; RNA-Seq
Fly heads were obtained from samples of 50-60 flies through flash freezing with liquid nitrogen, followed by vortexing and separation through a series of a sieves. Fly heads were then suspended in homogenization buffer and nuclei released into solution through chemical and physical agitation of the cell membrane. A whole head fraction (WH) was then taken from this homogenate as a representation of the whole fly head, containing both MB-specific GFP nuclei untagged non-MB nuclei. Anti-GFP bound beads were then used to isolate GFP-positive nuclei from the whole head homogenate to represent the mushroom body-specific fraction (MB). RNA was then isolated from both WH and MB-fractions using a PicoPure RNA Isolation Kit (Invitrogen:KIT0204) according to the manufacturer instructions. RNA libraries were generated using the Nugen Ovation Drosophila RNA-Seq System 1-16 (Nugen: NU035032) according to manufacturer instructions.
GEO
GSM3188282
GEO Accession
GSM3188282