GSM4088809
GSM4088809: lib1_pos_d15; Homo sapiens; RNA-Seq
Cells were captured in the Harvard Medical School (HMS) Single Cell Core for scRNA-seq using inDrops technology, as follows. First cells were assessed for cell number and viability and resuspended in 1000 µL of 15% OptiPrep™ to allow for a homogenous resuspension and reduced clumping. Cell capture and library preparation were performed using a modified version of inDrops protocols (Klein et al., 2015; Plasschaert et al., 2018) involving encapsulation of cells into 3-nl droplets with hydrogel beads carrying barcoding reverse transcription primers. Following the within-droplet reverse transcription step, emulsions were split into batches of approximately 2,000 cells, frozen at -80C, and subsequently processed as individual RNA-seq libraries (see Table S10). Approximately 4,000 cells for each time point were encapsulated for scRNA-seq
GEO
GSM4088809
GEO Accession
GSM4088809