skn1_skbr3_rna_014
single cell dna seq of a mixture of 2 cell lines
For the single-cell RNA experiments, 1 ml of aqueous phase 2 contains 180 l of 40% AA/bis-acrylamide solution, 129 l of 40% Acrylamide solution,  80 l of 500 M Acrydite-polydT primer (/5ACryd//iSp18/AAGCAGTGGTATCAACGCAGAGTNNWNNNSTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT, Integrated DNA Technologies), 50 l of Tris-HCl (PH7.5), 50 l of 0.5M EDTA, 10 l of 20% Sarkosyl (Sigma-Aldrich, L7414), 150 l of SUPERase In RNase Inhibitor (Thermo Fisher Scientific, AM2696), 100 l of 10% NP-40 (Thermo Fisher Scientific, 28324), 30 l of 0.1M DTT, 60 l of freshly-made 10% APS and 161 l of H2O.
skn1_skbr3_rna_014
SUB6325769
skn1_skbr3_rna_014