GSM4367970
GSM4367970: Skin-Y-23; Homo sapiens; RNA-Seq
After a cold PBS wash, samples were minced with scissors into ∼1 mm3 pieces in PBS on ice and transferred into 15-ml centrifuge tubes, rinsed twice with cold PBS and incubated at 37°C for 1 hr in digestion solution (PBS supplemented with 2 mg/ml collagenase I, 2 mg/ml collagenase IV, 2 mg/ml dispase and 0.125% trypsin-EDTA). Tissue samples were then broken into single cell suspension by pipetting, and the suspension was passed through a 40-μm strainer (BD Falcon). The digestion was stopped by adding DMEM (Gibco) containing 10% fetal bovine serum (FBS, Gibco). Dissociated cells were collected by centrifugation at 300 g for 5 min at 4°C and resuspended in 5 ml cold PBS. The cells were washed twice as above and resuspended in cold PBS supplemented with 10% FBS. sc RNA-seq libraries were constructed as per the 10x Genomics protocol using GemCode Single-Cell 3′ Gel Bead and Library V2 Kit.
GEO
GSM4367970
GEO Accession
GSM4367970