GSM2940002
GSM2940002: 14-1L 12minRep2; Pseudomonas aeruginosa; RNA-Seq
At time points after the addition of phage chosen to reflect each phase of infection, cells were immediately halted with stop solution (1%, 9% ethanol, 90% sample Blasdel (2018), pelleted, and re-suspended in TRIzol to lyse cells as well as inactivate RNAses and start an organic extraction. The resulting nucleic acid was digested with TURBO DNAse to remove DNA, rRNA was removed with the Ribo-Zero kit. Stranded cDNA was prepared with the TruSeq Stranded mRNA Library Prep Kit ahead of sequencing according to manufacturers instructions
GEO
GSM2940002
GEO Accession
GSM2940002