GSM2356903
GSM2356903: S1-KCSTclone4; Homo sapiens; RNA-Seq
Spheres were harvested and washed with PBS twice and the cell pellets were snap frozen in liquid nitrogen. Total RNA was prepared with PicoPure RNA isolation kit (Thermo Fisher Scientific) according to the manufacturer’s protocol. RNA-Seq libraries were built using KAPA Stranded mRNA-Seq kit (KK8400, KAPA Biosystems, Wilmington, MA) according to the manufacturer’s recommendation. Barcoded libraries were multiplexed and sequenced as single-end 75bp reads on Illumina NextSeq 500 sequencer.
GEO
GSM2356903
GEO Accession
GSM2356903