Rad21 ChIP rep2
Rad21 ChIP replicate 2
Acetylated and total cohesin were immunoprecipitated from HeLa cells with an AcSmc3 monoclonal antibody generated for our study and a commercial Rad21 antibody, respectively. HeLa cell lines expressing FLAG-tagged versions of Esco1 or Esco2 were used for ChIP of cohesin acetyltransferases with FLAG antibody. Whole cell extract DNA was also purified for input samples. Sequencing was performed on an ABI SOLiD5500 platform and reads were aligned to hg19 using SOLiD Bioscope.
Memorial Sloan Kettering Cancer Center
Rad21 ChIP         rep2
Memorial Sloan Kettering Cancer Center
Rad21 ChIP rep2