GSM2945799
GSM2945799: Wild Type; Mus musculus; RNA-Seq
Mouse mammary glands were harvested from virgin WT (n=3) and Per2ldc (n=4) mice followed by a primary mammary epithelial cell isolation.  Glands were homogenized on a 10cm plate using scalpels and digested in DMEM/F12 containing 10% FBS and 2mg/ml collagenase A for 30 min at 37°C.  Samples were then treated with DNAseI and pulse spins performed to remove red blood cells. RNA was isolated from MECs using the High Pure RNA Isolation Kit (Roche, Mannheim, Germany). Illumina true seq
GEO
GSM2945799
GEO Accession
GSM2945799