p2bc04
"Test sediment sample: run number 1, pool number 2 (Two step PCR 2), barcode number 4."
"The starting DNA template material from the sediment sample was amplified using non-barcoded PCR primers for 20 cycles (in duplicate and subsequently pooled) followed by a 100 times dilution of the resulting PCR product. Next, in triplicate reproduction, and for every of the fifty barcode pairs, 1 µl of the diluted PCR product was used for 10 additional cycles of amplification with the respective barcoded primers. This batch is referred to as the 'two-step PCR'. This is the second such pool of 50 replicates."
p2bc04
Uppsala University
p2bc04