GSM3188109
GSM3188109: CN12_SMA_ko_p10_M258_LCM255; Mus musculus; RNA-Seq
Brains and spinal cords were quickly dissected and snap frozen in 2-Methylbutane on dry ice. Tissues were sectioned in a cryostat and mounted onto PEN membrane glass slides (Zeiss). Quick staining with Histogene (Arcturus/LifeTechnologies) followed by dehydration in rising EtOH concentrations was performed immediately prior to LCM. Individual cells were isolated using a Leica LMD7000 system. Approximately 150 cells were collected into one tube and lysis buffer was added (0.2% triton-X 100 with 2U/ul RNase inhibitor, Clontech) before snap freezing the samples on dry ice. (Nichterwitz et al. 2016) Smart-seq2 combined with Tn5 tagmentation
GEO
GSM3188109
GEO Accession
GSM3188109