PM1-RB2
RNA-seq of Talaromyces marneffei PM1, SDA, 25C°C->37°C for 6hrs, replicate 2
At the beginning of the experiment, conidia of strain PM1 were inoculated in SDA plates and cultured at 25°C for a week. The germinated cells were transferred into new SDA plates every week for 2 weeks to establish stable colonies in mycelia growth form. One week before the extraction of total RNA, in order to obtain fresh cells, the homogenous cells were cultured in new SDA plates. Mycelia growth plates were transferred to 37°C for 6 hours before extraction. The total RNAs were extracted using the E.Z.N.A. fungal RNA kit (Omega Bio-Tek), following the manufacturer’s instructions with DNase I digestion to eliminate genomic DNA.
RB2
Texas A&M University
PM1-RB2