GSM3955342
GSM3955342: WT_27C; Arabidopsis thaliana; RNA-Seq
For RNA extraction, plants were grown on MS plates under long days conditions (16h light and 8h dark), at specified temperatures. All samples were collected at ZT16 and RNA was extracted using Plant RNA Purification Reagent (Invitrogen) following manufacturer's protocol. Library construction was carried out for RNA sequencing using Illumina TruSeq Stranded Total RNA Sample Prep kit according to manufacturer's manual.   After library construction and normalization, sequencing was conducted as paired-end reads on an Illumina HiSeq2000 sequencer. We sequenced mRNA of WT, upf1-5, upf3-1 and their double mutant seedlings grown at 16C and 27°C, day 8 after germination, ZT 16
GEO
GSM3955342
GEO Accession
GSM3955342