GSM2940104
GSM2940104: Hi-C_monkey_RS_rep1; Macaca mulatta; Hi-C
STA-PUT velocity sedimentation cell separator (Chamber Cat#56700-012, ProScience INC, Canada) was applied to generate separation of suspensions of living spematogenic cells on the basis of differences in cell sedimentation rate through the gravity, followed the methods developed for mouse testis cells (Bryant et al., 2013) and the manual of STA-PUT (2010 edition, ProScience INC) . For adult monkeys（5Y）, 2-5×107cell/ml testis cell suspension in 1600ml was used with 3hr sedimentation. For neonate monkeys (0.5Y), 600 ml cell suspension was used with 1.5hr sedimentation. According to the visible sedimentation bands, cell suspension was gradually collected through an automatic collector (Cat#DBS-160F, Jinke, China) after velocity sedimentation. All steps for separation were performed at 4°C.And the DNA extraction follows Hi-C protocol. Hi-C libraries were prepared using Hi-C protocal developed by Rao et al. with slightly modification TruSeq
GEO
GSM2940104
GEO Accession
GSM2940104