GSM3032543
GSM3032543: ChIP-exo, CEBPa, replicate 2 [mouse]; Mus musculus; OTHER
Cultured cells and liver tissue were crosslinked in 1 % formaldehyde for 15 minutes. A 5 minute quench was performed with 125mM glycine. To prepare ChIP extracts, nuclei were prepared by hypotonic lysis followed by Dounce homogenization. Nuclei were resuspended in SDS lysis buffer (50 mM HEPES/NaOH pH 7.5, 1% SDS, 10 mM EDTA, Complete protease inhibitor), incubated at 4 C for 10 min, and subjected to microtip probe sonication under conditions optimized for IP efficiency. ChIP-Seq libraries were produced and sequenced according to Illumina protocols. Libraries were multiplexed for sequencing.
GEO
GSM3032543
GEO Accession
GSM3032543