Leaf-transcriptome
Leaf transcriptome of Andrographis paniculata
Library  preparation  was  performed  at  Genotypic Technology’s  Genomics  facility  following  Illumina  TruSeq RNA library protocol outlined in “TruSeq RNA Sample Preparation Guide” (Part # 15008136;  Rev. A; Nov 2010). 1ug of Total RNA was subjected to Poly A purification of mRNA. Purified mRNA  was  fragmented for 4 minutes at elevated temperature (94oC) in the presence of divalent cations and  reverse  transcribed  with  Superscript  III  Reverse  transcriptase  by  priming  with  Random  Hexamers.  Second strand cDNA was synthesized in the presence of DNA Polymerase I and RnaseH. The cDNA  was cleaned up using Agencourt Ampure XP SPRI beads (Beckman Coulter). Illumina Adapters were  ligated to the  cDNA molecules after end repair and addition of A base. SPRI cleanup was performed  after  ligation.  The  library  was  amplified  using  8  cycles  of  PCR  for  enrichment  of  adapter  ligated  fragments.
LEAF
CSIR-Central Institute of Medicinal and Aromatic P
Leaf-transcriptome