PT_CH
16S-seq of water in Portugal temp 70
1st The DNA of the water samples was extracted with the DNeasy PowerWater kit (Qiagen, GER). 2nd Amplification and preparation of amplicons libraries with the following primers (5'CCTACGGGNGGCWGCAG-3 '), (5'GACTACHVGGGTATCTAATCC-3'), to allow amplification of the V3-V4 regions of the 16S rRNA gene. 3rd sequencing, performed through the Illumina MiSeq platform using the "MiSeq Reagent Kit v3" Kit, in a 2 x 300 bp paired-end system.
PT_CH
SUB4875222
PT_CH