E-MTAB-8144:LA2_31414_CCGTCC_p
Illumina HiSeq 2500 paired end sequencing; Comparison of tRNA expression between HEK293 and HeLa cells
Comparison of tRNA expression between HEK293 and HeLa cells
LA2_31414_CCGTCC_p
Cells were maintained in 60mm dishes at 37 °C in a humidified atmosphere at 5% CO2 in DMEM 4.5g/L Glucose with UltraGlutamine media supplemented with 10% of Tet-free FBS (Clontech) and 1% penicillin/streptomycin. Cells were washed twice with PBS and added 700µL of Trizol and continue with the extraction protocol.  Total RNA from HEK293 and HeLa was extracted using the miRNeasy Mini kit. For each sample, we obtain a yield of 20 µg of total RNA. 20 μg of 60-100 nt-sized total RNA were hydrolyzed during 10 min. was treated following the protocol of hydro-tRNAseq (Gogakos et al. 2017)   cDNA library preparation protocol as described previously (Garzia et al. 2016).
Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Dr. Aiguader 88, Barcelona 08003, Spain
E-MTAB-8144:LA2_31414_CCGTCC_p
Experimental Factor: cell line
HeLa