GSM3638646
GSM3638646: NSUN2 KO Ovary rep2; Drosophila melanogaster; Bisulfite-Seq
500-1,000 embryos, ~50 larvae, ~50 pupa, ~50 ovaries, and ~50 adult flies were used for RNA isolation in each stage.  Total RNA was isolated with TRIzol reagent and Direct-zol RNA MiniPrep kit. Polyadenylated RNA was separated from total RNA using Oligo dT Magnetic Beads (Vazyme). 100ng-1μg of polyadenylated RNA was converted using the EZ RNA methylation kit (Zymo Research) with modified high-stringency conversion conditions. The quantity of the converted RNA was determined by Qubit. 20ng converted RNA was fragmented into 150~200 nt fragments by incubation at 94 °C for 8min in fragmentation buffer (NEBNext Ultra II Directional RNA Library Prep Kit, NEB). The fragmented RNA was then used for library construction, following the manufacturer's protocol (NEBNext Ultra II Directional RNA Library Prep Kit, NEB).
GEO
GSM3638646
GEO Accession
GSM3638646