qiime_experiment_2192:1207551:570
unspecified sequencing; qiime_experiment_2192:1207551:570
study of families in their homes including microbial sampling of surfaces and human samapling (skin, gut).
H07a.Kitchen.Counter.3625:570
This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to ??1,500 samples per lane, and both PCR primers contain sequencer adapter regions.
CCME-COLORADO
qiime_experiment_2192:1207551:570
Colorado Center for Microbial Ecology, University of Colorado at Boulder
qiime_experiment_2192:1207551:570
experiment_title
Gilbert_house_forensics_BE
target_subfragment
V4
sample_center
ANL
sequencing_meth
sequencing by synthesis
experiment_center
ANL
pcr_primers
FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
run_center
ANL
num_sequences
66975
platform
Illumina
library_construction_protocol
This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to ??1,500 samples per lane, and both PCR primers contain sequencer adapter regions.
experiment_design_description
study of families in their homes including microbial sampling of surfaces and human samapling (skin, gut).
row_number
570
target_gene
16S rRNA
study_center
ANL