E-MTAB-6435:yWT1_s
Illumina HiSeq 2500 sequencing; Transcriptome profiling of liver samples of C/EBPß ∆uORF mice
Transcriptome profiling of liver samples of C/EBPß ∆uORF mice
yWT1_s
Liver tissue from young (5 months) and old (20 months) wt and C/EBPβΔuORF mice (from six individuals per group) was homogenized on ice with a motor driven pellet pestle (Kontes) in the presence of QIAzol reagent (Qiagen) and total RNA was isolated as described in Zidek et al., 2015. RNA was isolated from tissue samples using the RNeasy® Lipid Tissue Mini Kit (QIAGEN). After incubation for 30 min at 37°C with RQ1 RNase‐Free DNase (Promega), the RNA was further purified with the RNeasy® Plus Mini Kit (QIAGEN) according to the manufacture's protocol starting at step 4. Preparation of the sequencing libraries was performed using the TruSeq Sample Preparation V2 Kit (Illumina) according to the manufacturer's instructions.
European Research Institute for the Biology of Ageing, University of Groningen, UMC Groningen, The Netherlands
E-MTAB-6435:yWT1_s
Experimental Factor: genotype
wild type genotype
Experimental Factor: age
4