GSM3032681
GSM3032681: an007_KO; Mus musculus; RNA-Seq
SVZs were microdissected, dissociated to single cells, debris and dead cells removed. The single-cell suspension was subjected to Drop-seq. Drop-seq and single-cell library generation was performed as described in Alles et al., 2017, BMC Biol 15:44 (based on Macosko et al., 2015, Cell 161, 1202–1214). Drop-seq libraries were sequenced in paired-end mode on Illumina Nextseq 500  sequencers with 1% PhiX spike-in for run quality control. We used Illumina Nextseq500/550 High  Output v2 kits (75 cycles). We sequenced in paired-end mode: Read 1: 20 bp (bases 1-12 cell barcode, bases 13-20 UMI); Read 2: 64 bp; Index read: 8 bp.
GEO
GSM3032681
GEO Accession
GSM3032681