GSM3445701
GSM3445701: in vivo MII oocyte 3; Bos taurus; Bisulfite-Seq
Individual gamtes and embryos were lysis in a single tubes to obtain DNA using protease. We conducted DNA extraction, MspI digestion, end-repair, dA-tailing, adaptor ligation, bisulfite conversion, and PCR amplification libraries in single tubes to minimize DNA loss. Multiplex libraries using whole genome bisulfite sequencing approach.
GEO
GSM3445701
GEO Accession
GSM3445701