GSM1155711
GSM1155711: 517A ILW backfat, miRNA; Sus scrofa; RNA-Seq
Total RNA containing the small RNA fraction was extracted with Trizol (Invitrogen) according to the manufacturer's instruction. Results of the extraction were quantified using a Nanodrop ND-1000 spectrophotometer (Nanodrop Technologies) and the quality of the extracted RNA was assayed using an Agilent 2100 BioAnalyzer (Agilent Technologies). The miRNA library was prepared using the TruSeq Small RNA kit (Illumina) and version 3 of the reagents, following the manufacturer's suggested protocol. The library was sequenced on an Illumina HiSeq 2000 as 50 bp long single-reads using half lane per sample.
GEO
GSM1155711
GEO Accession
GSM1155711