BB157
BLISS of mus musculus MEFs wild type
100 ng of sonicated template was used for T7-mediated IVT using the MEGAscript T7 Transcription Kit (ThermoFisher #AMB13345), according to the manufacturer's guidelines. After RA3 ligation, reverse transcription was performed with Reverse Transcription Primer (RTP). Libraries amplification was carried out with NEBNext Ultra II Q5 Master Mix (NEB #M0544), RP1 common primer, and a selected RPIX index primer. Libraries were amplified for 8 PCR cycles, purified with a 0.8x AMPure XP bead purification, and then amplified for 4 additional PCR cycles. Then, the amplified libraries were cleaned-up according to the two-sided AMPure XP bead purification protocol, aiming at retaining library sizes from ~300800bp. Final library profiles were assessed and quantified on a BioAnalyzer High Sensitivity DNA chip and using the Qubit dsDNA HS Assay kit (ThermoFisher #Q32851).
BB157
SUB6037131
BB157