43_S43
Metagenomics analysis of crude oil contaminated soil samples
DNA from the oil contaminated soil samples were puriedusing MoBiokit. The QC passed samples are amplied using 16S rRNA gene universal primers (Forward Primer TCCGTAGGTGAACCTGCGG, Reverse Primer TCCTCCGCTTATTGATATGC) with standard illumina barcodes and adapters. The Amplicons are further puried using AmpureXPbeads. The barcoded libraries were validated by Agilent DNA HS Bioanalyser, and quantied using Qubit DNA BR reagent assay. The quantied libraries are pooled and sequenced in MiSeq with a read length of 251x2 bp.
43_S43
SUB6899571
43_S43