miRNA_Sample 4
OA-1
Small RNAs libraries were constructed from 1 g of total RNA from each sample using the Illumina TruSeq small RNA Sample Prep Kit (Illumina Inc., San Diego, CA, USA) according to the manufacturers protocol. PCR amplification was performed for 15 cycles. Library quality was determined using the High Sensitivity DNA Chip and an Agilent 2100 Bioanalyzer (Agilent Technologies) and quantified with qPCR with the KAPA Library Quantification kit (KAPA Biosystems, Foster City, CA, USA). The individual libraries were adjusted to 20 pM concentrations; sequencing was performed using a Miseq Reagent Kit v3 for 150 cycles in an Illumina Miseq Sequencing System (Illumina Inc., San Diego, CA, USA). This kit allows the generation of 25 million sequences reads per flow cell.
miRNA_Sample 4
SUB5080063
miRNA_Sample 4