PM1-PacBio
Genome sequencing of Talaromyces marneffei PM1 by PacBio RS
The P. marneffei strain PM1 was cultured in Sabouraud’s Dextrose Agar (SDA) at 25°C for 7 days. Sporulating colonies were covered by 1 ml of sterile phosphate buffed saline (PBS) containing 0.05% (v/v) TWEEN-20. The resulting mixture of conidia and hyphal fragments is withdrawn and filtered by Miracloth. The density of conidia in filtrate was adjusted to 1×10^8 conidia/ml. 100 µl of adjusted conidial suspension was inoculated into 50 ml of Sabouraud’s Dextrose Broth (SDB). After cultured at 37°C for 7 days, 5 ml of culture was transferred into another 45 ml of SDB and cultured at 37°C for 16 hours. Cells were collected by centrifuged at 3000×g for 5 min. Genomic DNA was extracted using E.Z.N.A. Fungal DNA Kit (Omega Bio-Tek Inc.), following the manufacturer’s instructions with a slight modification that RNA was digested by RNase I instead of RNase A.
PM1Pac
Texas A&M University
PM1-PacBio