R1 WXS
WXS of CT26 tumor responder to GAd and anti-PD1 treatment
For exome sequencing on mouse tissue, 25-30 mg of tumors were cut into small pieces, and placed in a 2ml microcentrifuge tube containing ATL buffer (Qiagen) and Proteinase K (20 l, Qiagen). Tissues were homogenized in the TissueLyser LT (speed 30 Hz, 3 min with 1 bead) and then placed into thermomixer (at 56C, shaking speed: 900rpm, Eppendorf) for 2 hours. After homogenization, 4 l RNase A (Qiagen) were added and samples were incubated for 2 min at room temperature before DNA extraction using the DNAse blood and tissue kit (QIAGEN). Genomic DNA was fragmented and used for Illumina library construction. Exonic regions were captured in solution using the Agilent mouse SureSelect All Exon kit 50Mb. Paired-end sequencing, resulting in 100bp from each end of fragments, was performed at target coverage of 120X.
R1 WXS
SUB5604304
R1 WXS