2b-RAD
2b-RAD reads of Ussuri catfish
Genomic DNA of each individual was firstly digested using the restriction enzyme BcgI, then digested DNA was ligated with adapters and  amplified using Phusion high-fidelity DNA polymerase. Amplicons were separated by electrophoresis in 8% polyacrylamide gels and purified, and then a final library was made by mixing equal amount of products from all libraries and sequenced on a lane at the Illumina HiSeq2500 platform.
2b-RAD
SUB5096468
2b-RAD