GSM4199198
GSM4199198: Wild Type Dissected Gonads sRNA 20C, Rep 2; Caenorhabditis elegans; miRNA-Seq
sample collection: Dissected gonads were collected in Trizol on ice over the course of 8-10 hours, then stored at -80 C. The Trizol containing the gonad tissue underwent 2 freeze/thaw cycles using liquid nitrogen prior to Trizol/chloroform extraction. Small RNAs (~16-30-nt) were purified from total RNA by size selection using 17% polyacrylamide gels and treated with RNA polyphosphatase to facilitate capture of 22G-RNAs. Libraries were then prepared according to the NEBNext® Multiplex Small RNA kit protocol. PCR products (~134-148 bp) were size selected using 10% polyacrylamide gels.
GEO
GSM4199198
GEO Accession
GSM4199198