GSM4452823
GSM4452823: s082826; Mus musculus; RNA-Seq
Cells were randomly selected into precooled lysate. Then, the lysate were shocked at 72℃ for 3min to release RNA and Initial denaturation. We used an improved Smart seq2 method. In brief, single-cell suspension is firstly prepared, and then single cells are selected into a PCR tube containing oligo(dT) primers and lysates through a suction tube.After cDNA amplification, the single cells labeled with different barcodes can be pooled together for subsequent library construction and sequencing. We add biotin in our indexed primers which can pair with 3' cDNA primers, so after additional amplifying of pooled cDNAs following shearing to about 300bp , we use Dynabeads® MyOne™ Streptavidin C1 beads to enrich for the 3' terminal of cDNAs. Then the library was constructed by using Kapa Hyper Prep Kit(Thermo Fither) and run on illumina Hiseq X Ten for 150bp pair-end reads.
GEO
GSM4452823
GEO Accession
GSM4452823