GSM3187526
GSM3187526: Control_37; Oncorhynchus mykiss; RNA-Seq
Gill samples placed in 1.5 mL tubes containing Lysis Solution and 2-mercaptoethanol were homogenized by TissueLyser II (Qiagen, USA). Total RNA was extracted using RTN350 (Sigma-Aldrich), according to the manufacturer's instruction and subsequently, DNase treated with DNase I (Thermo Scientific, USA) Quality and integrity of the total RNA were checked on an Agilent Bioanalyzer 2100 total RNA Nano series II chip (Agilent, Amstelveen, Netherlands). Illumina RNAseq libraries were prepared from 500 ng total RNA using the Illumina TruSeqTM Stranded mRNA LT Sample Prep Kit according to the manufacturer's instructions (Illumina Inc. San Diego, CA, USA).
GEO
GSM3187526
GEO Accession
GSM3187526