E-MTAB-8391:Q334_A2_s
NextSeq 500 sequencing; Comparison of fibroblasts from ARH3-mutated patients and controls by transcription profiling.
Comparison of fibroblasts from ARH3-mutated patients and controls by transcription profiling.
Q334_A2_s
Samples were obtained from fibroblasts cell cultures originating from patient's skin. Total RNA was isolated from 5x10^5 harvested cells by RNeasy Micro Kit (Qiagen), according to the procedure for animal cells. Poly(A) RNA Selection Kit (Lexogen) was used for mRNA extraction. SENSE Total RNA-Seq Library Prep Kit (Lexogen).
Service Laboratory of Functional Genomics and Bioinformatics Institute of Molecular Genetics of the ASCR, v. v. i.
E-MTAB-8391:Q334_A2_s
Experimental Factor: genotype
ARH3 mutation