GSM2940694
GSM2940694: C3H-ISO-D21-Rep2; Mus musculus; RNA-Seq
Thy1+HE- cells were isolated from saline and ISO-treated hearts 14 or 21 days after pump implantation (n=2 per strain/condition), and then sorted directly into TRIzol® LS Reagent (Ambion). Total RNA was extracted using the RNeasy miniElute Cleanup Kit (Qiagen), according to manufacturer's instructions. The complementary DNA (cDNA) library was generated using reagents provided in the KAPA mRNA Hyperprep kit. The amplified cDNA library was sequenced on an Illumina HiSeq 3000 according to manufacturer's instructions. Libraries were sequenced using 50 single-end read protocol
GEO
GSM2940694
GEO Accession
GSM2940694