98AG31
Host: P. x interamericana 'Beaupré' location: 49.7500 N 3.3500 E: Sample 98AG31
"Genomic DNA libraries were prepared using TruSeq DNA sample preparation kit (v3) followed by paired-end 100bases massively parallel sequencing on Illumina HiSEQ2000 by Integragen (Evry, France). Briefly, 3µg of each genomic DNA were fragmented by sonication and purified to yield fragments of 400-500 bp. Paired-end adapter oligonucleotides from Illumina were ligated on repaired A-tailed DNA fragments, then purified and enriched by PCR cycles. Each library was quantified by qPCR and sequenced on Illumina HiSeq2000 platform as paired-end 100b reads. Image analysis and base calling were performed using Illumina Real Time Analysis (RTA 1.13.48.0) pipeline with default parameters"
98AG31
INRA
98AG31
alignment_software
CLC workbench genomics