GSM1437487
GSM1437487: mmu_ezh2_smRNA_2; Mus musculus; miRNA-Seq
Total RNA was isolated from cells using Trizol reagent (Invitrogen) according to the manufacturer’s instructions. 10 µg of total RNA was size selected to 18-40 nucleotides on a denaturing polyacrylamide gel, eluted in 0.3M NaCl for overnight and then ethanol precipitated. Small RNAs (18-40 nt) precipitate were dissolved into water. Small RNA libraries were prepared according to manufacturer’s instructions (SOLiD small RNA library preparation kit, Life Technologies). All libraries were sequenced using SOLiD instrument (Life Technologies).
GEO
GSM1437487
GEO Accession
GSM1437487