Bgl3_Lib2_unsorted
High-temp DMS of Bgl3 from Streptomyces,  Library2, unsorted
A GH1 gene library was generated via  error-prone PCR, cloned into an expression vector, and transformed into BL21  (DE3) cells. This transformed library was then grown in a liquid culture, the  plasmid DNA was extracted, the GH1 gene insert was digested out of the  expression vector, and isolated via gel extraction. The GH1 gene insert was  then randomly fragmented using the Nextera XT kit and sequenced using a 2x300  MiSeq v3 kit.
Bgl3_Lib2_unsorted
SUB7240601
Bgl3_Lib2_unsorted