CHM1_Illumina_WGS_UW
CHM1 Illumina WGS (UW)
CHM1htert gDNA was sheared using Covaris S2 with cycling conditions of 10% Duty cycle, Intensity 4, Cycles/Burst 200, and Time 100s. The sheared DNA was then end-repaired using NEBNext End Repair Module (P/N: E6050L). Repaired sheared DNA was then A-tailed and Y-adapters were ligated. The library was size-selected at a range of 450-550 bp then sequenced using Illumina HiSeq PE-101 to generate ~41-fold sequence coverage.
Eichler-CHM1htert.1_500
UWGS-EL
CHM1_Illumina_WGS_UW