GSM3105122
GSM3105122: s2_Vecadpos_GFPneg; Mus musculus; RNA-Seq
Cells from miR144/451+/GFP E11.5 YS were FACS sorted into tube strips with lysis buffer containing 0.2% Triton X-100, oligo-dT Primer and dNTP mix and after snap frozen on dry-ice. RT & template-switching, PCR preamplification (14 cycles of amplification) were performed as described in the Smart-seq2 protocol (Picelli S et al, 2014, PMID: 24385147 ) RNA libraries were prepared for sequencing using standard Illumina protocols
GEO
GSM3105122
GEO Accession
GSM3105122