Psz214
Identyfication of SNP markers using SBG (sequence-based genotyping) method
Wheat genomic DNA were digested with two restriction enzymes: TaqI and PstI and then ligated with compatible adapters. The adapter sequences were based on P5 and P7 Illumina Nextera XT oligonucleotides sequences. After ligation, the samples were PCR amplified using primers complementary to P5-TaqI and P7-PstI adapters. The PCR reactions were purified and then used for the index PCR reactions. Prior to sequencing, the proubes were pooled into 32 pools and subjected to size selection with recovery of 200-400bp fragments.
Psz214
SUB5082891
Psz214