IL1564-SU070-Tcell
Majeti 2012 - T cell AML Exome
Library was made as follows: 1) Isolation of gDNA from FACS-purified cells using QIAgen QIAamp DNA mini kit. 2) Shearing of gDNA using Covaris S2 (10% duty cycle, Intensity = 5200 cycles per burst, 180 seconds/sample, Acoustic power between 17 and 19, temp between 4 and 6 degrees C) 3) Illumina paired-end library prep using NEBNext DNA Sample Prep Master Mix Set 1 Kit (cat # E6040L) 4) Exome capture using Nimblegen SeqCap EX Exome Library SR Kit v2
SU070-Tcell
Stanford University
IL1564-SU070-Tcell