36ppt_3day
3 day post-hach larvae incubated at 36 ppt
Fertilized eggs of Senegalese sole were collected from spontaneous spawn at “El Toruño” facilities (El Puerto de Santa María, Cadiz). Temperature in the broodstock during the spawning was approximately 18.5ºC and salinity 34 ppt. Eggs were transferred to a 1,000-mL measuring cylinder to separate buoyant (viable) from non-buoyant (non-viable) eggs and the number in each fraction was estimated using volumetric methods (1,100 eggs mL-1). After estimating the number of fertilized eggs, we incubated embryos (at the gastrula stage) in 15-L cylinder tanks at an initial density of 2,000 embryos L-1. After seeding, two salinities (10 and 36 ppt) were established using a recirculation system that kept constant temperature (20ºC±0.5) and target salinity. Water turnover was maintained at one total renewal per hour during the experiment. Trial was done in duplicate tanks for each salinity. After hatching, larvae were sampled at day 1 and 3 after hatch using a 350 µm-mesh net. Three pools of larvae were collected from each tube (~100 larvae/pool), washed with DEPC water, frozen directly in liquid nitrogen and stored at -80 °C until analysis.
36ppt_3day
Universidad de Málaga
36ppt_3day