GSM2694179
GSM2694179: Hi-C GM12878; Homo sapiens; Hi-C
For Hi-C, about 5M cells were crosslinked with 1% formaldehyde for 10 min at room temperature. Formaldehyde was quenched  by 0.2M glycine. Nuclear lysates were incubated with MboI. Fragment ends were filled with biotinylated nucleotides, then ligated with T4. Ligated fragments were reverse crosslinked, purified, sonicated and size selected before Illumina libraries generation. Sequencing libraries were generated using NEBNext Ultra DNA Library Prep Kit for Illumina (E7645, NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.
GEO
GSM2694179
GEO Accession
GSM2694179