GSM2942296
GSM2942296: SA2_MCF10A_ChIP-seq_siSA2_Rep_2; Homo sapiens; ChIP-Seq
IP step of the experiment was performed adding 20% of mouse chromatin to human chromatin (600ug)- 720ug in total per replicate.  72Ug of antibody was used per replicate. Samples went through subsequent enzymatic treatments of end-repair, dA-tailing, and ligation to adapters with "NEBNext Ultra II DNA Library Prep Kit for Illumina" from New England BioLabs (catalog # E7645). Adapter-ligated library was completed by limited-cycle PCR and extracted with a [single] double-sided SPRI size selection. Median fragment size is 360 bp from which 120 bp correspond to adaptor sequences. Library was applied to an Illumina flow cell for cluster generation and sequenced on an Illumina instrument (see below) by following manufacturer's protocols.
GEO
GSM2942296
GEO Accession
GSM2942296