GSM1437645
GSM1437645: b9_3; Malus domestica; miRNA-Seq
The samples (approximately 0.5 g each) included the meristem, stem and the leaves but not including the first fully expanded leaf. Shoot tips were flash-frozen in liquid nitrogen and stored at -80°C for later RNA isolation.  Samples were ground with a mortar and pestle chilled with liquid nitrogen. The frozen powder was mixed with extraction buffer (2% Cetyltrimethylammonium bromide, 2% polyvinylpyrolidone, 100 mMTris pH 8.0, 25 mMethylenediaminetetraacetic acid, 2 M NaCl, 0.5 g L-1 (w/v) spermidine, 2% ß-mercaptoethanol) and total RNA was isolated using 0.8 M LiCl and isopropanol (Sigma).  Total RNA concentration and purity were determined using NanoDrop (Thermo Fischer Scientific). Total RNA quality was determined on BioAnalyzer (Agilent). Small RNA libraries were prepared using the Applied Biosystems (Life Technologies) Total RNA-Seq Kit according to the Small RNA Library Preparation protocol.
GEO
GSM1437645
GEO Accession
GSM1437645