Noncoding RNA Project
Strand-specific RNA sequencing of Rsc mutant budding yeast- tet-sth1, replicate1
Strand-specific RNAseq libraries created using the dUTP method. Strain was grown in YC(-His) with doxycycline added to 20ug/ml at 37 degrees C until OD600 ~0.4-0.7.  Total RNA was isolated using acid phenol extraction.  rRNA was depleted and libraries were creating using TruSeq.  MATa his3-1 leu2-0 met15-0 ura3-0 tetR(HIS3)-STH1-FLAG
Fred Hutchinson Cancer Research Center
Noncoding RNA Project