SL30332
RNA sample from human male participant M006 - CD4+ T-cells
"Peripheral blood mononuclear cells were fractionated by positive selection on antibody-coated magnetic beads into CD4+ T cell, CD8+ T cell, and CD19+ B cell populations. Purities of the resulting populations (>90%) were confirmed by flow cytometry. Sample preparation metadata, including live cell counts, cell purity, and time before freezing were recorded. RNA was purified, libraries prepared and sequencing (50 million reads/sample) performed using the Illumina HiSeq 2000 platform (HudsonAlpha Genome Services Laboratory, Huntsville, AL, USA). Sufficient RNA could not be obtained for one subject, who was excluded from subsequent analyses. Samples were prepared and sequenced in three pools with paired-end 50bp reads "
SL30332
UFL-phs001426
SL30332