DF-05-25-2016
DNA sequencing of wastewater: collected on 5-25-2016, filtered through 0.4 um filter and 0.22 um filter
From the Urbana & Champaign Sanitary District Northeast Plant (Urbana, IL), 1 L of unprocessed wastewater was collected at each of three time points: May 25, 2016, June 23, 2016, and October 3, 2016. Wastewater samples were transported on ice, immediately centrifuged at 2,500  g for 10 min at 4C and filtered through a 0.4 m polyethersulfone filter to remove large particulate and cellular matter. The sample was split into three aliquots and processed three ways. One aliquot was not processed further (F). Another aliquot was filtered a second time through a 0.22 m polyethersulfone filter (DF). The last aliquot was washed three times with an equal volume of chloroform (FC). All aliquots were concentrated 100-fold and virome DNA was isolated from each as described for genome sequencing of phages. DNA libraries of virome DNA were prepared using the same methods as described for genome sequencing and were sequenced on an Illumina HiSeq 2500 sequencer with a HiSeq v4 SBS sequencing kit (Illumina, San Diego, CA) producing 2160-bp paired-end reads. Fastq files of demultiplexed reads were generated with the bcl2fastq v2.17.1.14 Conversion Sotfware (Illumina, San Diego, CA). Reads were trimmed and quality filtered using Trimmomatic 0.38 (93).
DF-05-25-2016
SUB7209759
DF-05-25-2016