GSM3876316
GSM3876316: Btheta_colon_1; Mus musculus; RNA-Seq
Colons were were digested with collagenase/dispase to single cell suspensions. T cells were sorted by flow cytometry as CD45+ TCRb+ CD4+ cells. Single cells were then encapsulated in nanodroplets according to the InDrops protocol. mRNA was reverse transcribed using adapters containing oligo dT in each droplet. The emulsion was then broken and linear amplification was performed by in vitro transcription. Illumina adapters were added by reverse transcription and PCR. Library were sequenced using a NextSeq500.
GEO
GSM3876316