31224_GTCCGC
ChIP-Seq of H4K16ac: adult female (Input), CD3+ cells Donor 3
For high-throughput sequencing of H4K16ac-enriched genomic regions (ChIP-seq), CD3+ T cells and neutrophils isolated from human peripheral blood were freshly processed using the SimpleChIP Enzymatic Chromatin IP Kit (Magnetic Beads) (Cell Signaling, 9003). The amount of Microccocal Nuclease required to digest DNA to the optimal length was determined as 2.5 l per 2 x 107 cells. Library preparation and sequencing were performed at Centre de Regulaci Genmica (CRG) in Barcelona on an Illumina HiSeq 2000 system in 50-single end mode.
31224_GTCCGC
SUB5214343
31224_GTCCGC