GSM2174867
GSM2174867: ura4+::priRNA1 ago1∆ + OE Flag-Ago1 WT (spy3473 + pDM817) - sRNA sense; Schizosaccharomyces pombe; ncRNA-Seq
Total RNA was isolated using the mirVana kit (according to the manufacturer protocol) Small RNA libraries were constructed as previously described (Halic and  Moazed, 2010). Briefly, 21-24nt RNA was size-selected on a 17.5% polyacrylamide/7M  urea gel and ligated to a 3' adapter. The ligated species were size-selected on a 17.5%  polyacrylamide/7M urea gel and ligated to a 5' adapter. RNA was then reverse  transcribed into cDNA and PCR-amplified in a two-step process. Amplified cDNA was  gel-purified and sequenced on an Illumina High-Seq platform.
GEO
GSM2174867
GEO Accession
GSM2174867