RNA-seq of Hycleus cichorii
Comparative analysis of female and male in Hycleus cichorii
Total RNA (~10 μg) was extracted from single insect using the TRIzol Reagent (Invitrogen). RNA samples were subjected to DNase I digestion to digest any remaining DNA. The Poly-A RNA was extracted using poly-T oligo-coated magnetic beads (Invitrogen). After purification, the first-strand cDNA was synthesized using Superscript II reverse transcriptase (Invitrogen) and random hexamer primers. The cDNA was further converted into double stranded DNA. The library constructed using TruSeq®RNA sample prep kit (Illumina). After quality control of the librarie, pair-end sequencing analysis was carried out using Illumina HiSeq™ 4000 at the Beijing Genomics Institute in Shenzhen.
single beetle
Guizhou Medical University
RNA-seq of Hycleus cichorii