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RNA-Seq of C. albicans adherent cells
Overnight cultured C. albicans TDH3-GFP-CAI4 cells were adjusted to 106 cells/ml using synthetic medium plus dextrose medium and supplemented with 80 M farnesol. 200 l of aliquot was dispended into wells of 96-well plates (NEST). After 36 h incubation at 37 C with 100 rpm rotation, supernatants were discarded and wells were thoroughly washed three times with PBS to remove the non-adherent cells. The adherent cells in wells were scraped and collected for RNA extraction following with RNA sequencing.
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SUB4143687
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