GSM3187970
GSM3187970: Early embryo MNase-seq rep2; Caenorhabditis elegans; MNase-Seq
DNA was extracted using phenol-chloroform. The degree of MNase digestion was assessed using agarose gel electrophoresis and an Agilent 2100 Bioanalyzer. DNA extracted from the mononucleosome-enriched digestions that were comparable between sperm and early embryos was used for library contruction. Illumina sequencing libraries were constructed, using NEBNext Ultra DNA Library Prep Kit (NEB, E7370). During library construction, size selection was performed on adaptor-ligated DNA prior to amplification to remove fragments larger than 250 bp (40 μL of AMPure beads were used for 1st Bead Addition based on the manufacturers' instructions), and PCR-amplified DNA was purified using 1:1 AMPure XP beads, which removed fragments less than 100 bp.
GEO
GSM3187970
GEO Accession
GSM3187970