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16S amplicon of poultry litter
The 16S rRNA gene V1V3 region was amplified using previously published primers fused to barcodes and adaptor sequences for the MiSeq instrument.  Briefly, PCRs contained the following: 17 l AccuPrime Pfx SuperMix (Life Technologies, Grand Island, NY), 5.0 M each of the primers i5+V3 and i7+V1 , and 25 ng of DNA. The following PCR conditions were used: 2 min at 95 C, 22 cycles of [20 s at 95 C, 15 s at 55 C, 5 min 72 C], 72 C for 10 min.
S420
SUB6474497
S420