GSM3335828
GSM3335828: Sample 11_Human neutrophils, donor 11; Homo sapiens; RNA-Seq
RNA isolation RNeasey Mini Kit (Quiagen) according to vendor protocol. Samples 1-8 were not treated with extra DNAse, Sample 9-24 were treated with DNAse. Magnetic oligo-dT beads (NEB) were used for mRNA enrichment starting with 1 to 10 ng total RNA with RIN values above 9. The NEBNext Ultra II directional RNA Library prep Kit (NEB) was used to prepare RNA-Seq libraries according to the manufacturer's protocol. Paired-end sequencing was performed with 2x 75 cycles on an Illumina NextSeq 500
GEO
GSM3335828
GEO Accession
GSM3335828