GSM3188539
GSM3188539: Untreated rep2; Homo sapiens; RNA-Seq
Total RNA was extracted from the cells using TriPure Isolation Reagent (Roche Diagnostics GmbH, Germany) according to the kit's protocol. To avoid genomic DNA contamination, the RNA samples were treated with DNase for one hour. Following RNA isolation, creation of an RNA-seq library is the next step in transcriptome sequencing. The preparation of intended cDNA libraries and paired-end sequencing were performed by the Beijing Genome Institute (BGI), China. In total, four cDNA paired-end libraries were generated for transcriptome sequencing on Illumina HiSeq 2000 platform. Briefly, rRNA depletion methods were used for library construction. Based on the protocol, after rRNA depletion all other RNAs with or without the polyA sequence were subjected to adaptor ligation. The adapter-ligated templates were purified by the Agencourt AMPure SPRI beads and fragments with insert size about 200 bp were excised and used to synthesize the first strand cDNA.
GEO
GSM3188539
GEO Accession
GSM3188539