GSM3639109
GSM3639109: siMEF2C 3; Homo sapiens; ncRNA-Seq
Total RNA was extracted with TRIzol reagent (Invitrogen) from cells according to the manufacturer's protocol. Total RNA was purified by 12% PAGE, and a fraction of small RNAs of 10-30 nt in length was excised. Small RNA libraries were generated using a TruSeq Small RNA kit (Illumina) according to the manufacturer's instructions. Number of biological replicas per group: 3. To equalize the read depth, the libraries were multiplexed with four libraries sequenced per lane of Illumina HiSeq 2000.
GEO
GSM3639109
GEO Accession
GSM3639109