A.3
DWTR-suspended with aeration
The bacterial 16S rRNA V4-V5 fragments were amplified using forward primer 515F (5-GTGCCAGCMGCCGCGGTAA-3) and reverse primer 907R (5-CCGTCAATTCCTTTGAGTTT-3). PCR reaction was performed in the 50 L mixture that contained: 0.2 M of primer 515F, 0.2 MTimes New Roman
A.3
SUB5972385
A.3