GSM3955921
GSM3955921: FECM_Ependymoma_1.5mo_FBS; Homo sapiens; RNA-Seq
Samples were flash frozen in liquid nitrogen and stored in -80 oC in individual RNase free eppendorf tubes until RNA extraction was performed. All samples were placed on dry ice during extraction, sequentially disrupted using a liquid nitrogen chilled bio-pulverizer. Between each sample, the pulverizer was wiped with 70% ethanol to remove remnants of the previous sample, and between each sample set (different conditions), all the tools were cleaned with RNAzap. Lysis buffer was immediately added to the powdered frozen sample and placed on ice. Once all the samples were in lysis buffer on ice, a 22-gauge needle and syringe were used for sample homogenization one by one using a fresh needle and syringe every time. All the samples were spun down to remove undigested material (mainly silk scaffold) and the supernatant was transferred to clean RNase free eppendorfs. Following this, the SurePrep All Prep kit (Fischer Scientific) protocol was followed until RNA was eluted from the columns. KAPA mRNA stranded library.
GEO
GSM3955921
GEO Accession
GSM3955921