GSM3185650
GSM3185650: H3K27ac_Ctrl; Drosophila melanogaster; ChIP-Seq
We isolated 100 wing discs per sample (early control and regeneration). Discs were fixed, pooled in 700 μl of sonication buffer (10 mM Tris-HCl, pH 8.0, 2 mM EDTA and 1 mM EGTA) and processed as described (Pérez-Lluch et al. 2011). Immunoprecipitations were performed in RIPA buffer and using 1 μg of the corresponding antibody. Immunocomplexes were recovered by incubation with Invitrogen Protein A magnetic beads for 2 h. The beads were washed three times in RIPA, once in lithium chloride buffer and twice in TE buffer. After, an RNase treatment was done and samples were decrosslinked at 65⁰C overnight by adding Proteinase K. Samples were purified with Qiagen MinElute Kit and eluted in Gibco water. HiSeq2000
GEO
GSM3185650
GEO Accession
GSM3185650