GSM1436142
GSM1436142: Dnmt3a knockout MBD-seq; Mus musculus; MBD-Seq
Cells were lysed with genome buffer (50mM Tris-HCl pH8.0, 100 mM NaCl, 20 mM EDTA, 1% SDS, 140 μg/mL protenase K) and treated with RNase. Subsequently genomic DNA was purified with Phenol-Chloroform DNA extraction. Genomic DNA was sonicated to 200-500 bp fragment with Covaris (Covaris). Methylated DNA was enriched with EpiXplore Methylated DNA Enrichment Kit (Takara) according to manufacturer’s protocol. Multiplexed MBD-seq libraries were prepared from 10 ng of methylated DNA fragment using TruSeq ChIP Sample Prep Kit (Illumina). For sequencing using Hiseq2000, cluster generation was performed using TruSeq PE Cluster Kit v3 (Illumina). Sequencing was performed by a single-read run mode with total 200 cycles.
GEO
GSM1436142
GEO Accession
GSM1436142