D134_RAPCR
mRNA-Seq for Associative Transcriptomics in the polyploid crop species Brassica napus
D134_RAPCR
The Illumina mRNA-Seq kit (RS-100-0801, Illumina Inc.) was used according to the manufacturer's protocol to convert total RNA into a library of template molecules suitable for high throughput DNA sequencing for subsequent cluster generation. Libraries were prepared using 5 micrograms of total RNA, quantification and quality assessment were carried out by running 1 microlitre of library on an Agilent DNA 1000 LabChip (Agilent Technology 2100 Bioanalyzer).
TGAC
D134_RAPCR