1
soil
In this analysis, V3 region of the 16S rRNA gene was amplified using primer pair (341F-5CCTACGGGAGGCAGCAG3; 518R- 5ATTACCGCGGCTGCTGG3). Amplicons were purified and paired-end sequenced on an Illumina Hi-Seq platform. Singletons were removed as it might be a consequence of sequencing errors and can result in spurious OTUs. All the pre-processed reads were used to identify the OTUs using QIIME software package for constructing a representative sequence for each OTU.
1
SUB7240039
1