E-MTAB-6174:Mus438_p
Illumina HiSeq 2500 paired end sequencing; RNA-seq of muscle from pigs divergent in feed efficiency and product quality
RNA-seq of muscle from pigs divergent in feed efficiency and product quality
Mus438_p
The slaughter of animals, fasted for 18 hours with an average final body weight of 99 kg (SD: 11.4kg), occurred on 2 slaughter days a week apart by electronic stunning followed by exsanguination. Samples of the LTL muscle were collected and snap frozen in liquid nitrogen within 10 minutes post-mortem (pm) followed by storage at -80°C until RNA isolation. Residual feed intake is one method of measuring feed efficiency. In this study, residual feed intake (RFI) is described as the difference between actual feed intake and predicted feed requirements on product quality of the porcine Longissimus thoracis et lumborum (LTL) muscle. Pigs were placed on test between day 42 and 91 post-weaning, whereby feed intake was recorded daily, and pig weight, back-fat depth and muscle depth was recorded weekly. Average daily feed intake (ADFI) and average daily gain (ADG) from day 42 to day 91 post-weaning were calculated for each pig weekly. RFI was calculated at the end of the test period as the residual from a least squares regression model of ADFI on ADG, metabolic live weight, gender and also all relevant two-way interactions, and the effects of back-fat and muscle depth using the PROC REG procedure in SAS (version 9.4; SAS Inst. Inc., Cary, NC, USA). Total RNA was isolated using Tri-Reagent (Sigma-Alrich, Taufkirchen, Germany), followed by DNase treatment and a column-based purification using the Nucleospin RNA II kit (Macherey-Nagel, Düren, Germany). RNA library preparation was carried out using the TruSeq Stranded mRNA Library Preparation Kit (Illumina).
Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology
E-MTAB-6174:Mus438_p
Experimental Factor: phenotype
low residual feed intake