GSM4514044
GSM4514044: ATAC-seq_Fli1KO_rep2; Mus musculus; ATAC-seq
Sorted cells (2.5 x 104) were washed  twice in cold PBS and resuspended in 50μl of cold lysis buffer (10nM Tris-HCl, pH 7.4, 10mM NaCl, 3mM  MgCl2, 0.1% Tween). Lysates were centrifuge (750xg, 10min, 4oC) and nuclei were resuspended in 50μl of  transposition reaction mix (TD buffer [25μl], Tn5 Transposase [2.5μl], nuclease-free water [22.5μl];  (Illumina)) and incubated for 30min at 37oC. Transposed DNA fragments were purified using a Qiagen  Reaction MiniElute Kit, barcoded with NEXTERA dual indexes (Illumina) and amplified by PCR for 11  cycles using NEBNext High Fidelity 2x PCR Master Mix (New England Biolabs).
GEO
GSM4514044
GEO Accession
GSM4514044