Microcosm 1U61 T70
Impact of an alkaline microbiome on the mobility of uranium under conditions analogous to nuclear waste disposal
Sequencing of PCR amplicons of 16S rRNA was conducted with the Illumina MiSeq platform (Illumina, San Diego, CA, USA) targeting the V4 hyper variable regions (forward primer, 515F, 5'-GTGYCAGCMGCCGCGGTAA-3'; reverse primer, 806R, 5'-GGACTACHVGGGTWTCTAAT-3') for 2150-bp paired-end sequencing. PCR amplification was performed using Roche FastStart High Fidelity PCR System in 50ul reactions under the following conditions: initial denaturation at 95C for 2min, followed by 36 cycles of 95C for 30s, 55C for 30s, 72C for 1 min, and a final extension step of 5min at 72C. The PCR products were purified and normalised to 20ng each using the SequalPrep
Microcosm 1U61 T70
SUB5382039
Microcosm 1U61 T70