GSM4508858
GSM4508858: DOS-105; Canis lupus familiaris; Bisulfite-Seq
Tumor tissues were removed from -86℃ and sectioned to avoid areas of necrosis. Thirty mg of tissue were placed in 80 µL of phosphate buffered saline (PBS) solution and pulverized in a tissue homogenizer. Isolation of genomic DNA was done according to the manufacturer's protocol using the QIAamp DNA Mini Kit from Qiagen.Illumina library preparation, bisulfited converted and bead capture was performed as specified in the Roch SeqCap Epi protocol outlined in Li et al (doi: 10.1093/nar/gkv244)
GEO
GSM4508858
GEO Accession
GSM4508858