GSM3333101
GSM3333101: Ser3; Pseudomonas putida KT2440; RNA-Seq
Total RNA extraction and DNase I treatment for DNA removal were performed as described elsewhere (ref. 26 of the text). The integrity of total RNA, and DNA contamination were assessed with a RNA 6000 Nano chip on the Agilent 2100 Bioanalyzer (Agilent Technologies). Total RNA was extracted from three biological replicate cultures for each condition. Sequencing libraries were constructed using the Illumina® TruSeq® Stranded mRNA Sample Preparation kit. The libraries were sequenced twice on the Illumina HiSeq2000 platform (Beckman Coulter Genomics), 100 bp, single-end.
GEO
GSM3333101
GEO Accession
GSM3333101