LX22 RNA-Seq
LX22 Small cell lung cancer primary xenograft line. RNA-Seq experiment.
Total RNA was checked for quality and yield by automated microfluidic electrophoresis (Bioanalyzer 2100, Agilent Technologies, Santa Clara, CA, USA) and spectrophotometer (NanoDrop, Thermo Scientific, Wilmington, DE, USA). Non-directional RNA-Seq libraries were created according to the manufacturers protocol (Truseq RNA-Seq Library Prep Kit v2, Illumina, San Diego, CA, USA). Briefly this method involved sequential steps of mRNA enrichment from 3ug total RNA, RNA fragmentation by heating in the presence of using divalent cations, a randomly primed reverse transcription and second-strand cDNA synthesis followed by preparation of DNA fragment libraries using Illumina compatible adapters and PCR amplification as previously described for DNA libraries.
LX22
Monash Institute of Medical Research - Monash Univ
LX22 RNA-Seq