GSM2934751
GSM2934751: 3C: replicate B, 1 minute; Synechococcus sp. PCC 7002; RNA-Seq
RNA spike-ins were added to samples immediately before extraction with hot phenol and DNase-treatment as in RNA was extracted using the hot phenol method outlined in Khodursky A, Bernstein J, Peter B, Rhodius V, Wendisch V, Zimmer D. 2003. Escherichia coli spotted double-strand DNA microarrays: RNA extraction, labeling, hybridization, quality control, and data management. Methods Mol Biol 224:61–78. RNA was submitted to the University of Wisconsin Biotechnology Gene Expression Center for RNA-sequencing. rRNA was removed with a Ribo-Zero Magnetic Kit and the library was created using a TruSeq Stranded Total RNA Library Kit. Samples were run on an Illumina HiSeq 2500.
GEO
GSM2934751
GEO Accession
GSM2934751