B2
Targeted sequencing of homo sapiens: prostate cancer B2
Regions of tissue were excised from formalin-fixed paraffin-embedded tissues using laser capture microdissection and DNA was extracted using the QIAamp DNA FFPE Tissue Kit. 1-10ng of DNA were constructed into targeted sequencing libraries using 3 successive rounds of PCR to append Illumina Nextera adaptors to the 5' and 3' ends of the amplicon. Amplicon libraries were sequenced on a MiSeq with the v2 Reagent Kit with 195 cycles paired-end. Pass-filter FASTQ files were trimmed using Trimmomatic and aligned with BWA-MEM.
B2
NCI-phs001938
B2
alignment_software
BWA-MEM 0.7.17