NT22.2 Archaea-August 2009-2
NT22.2 Archaea-August 2009-2
The 16S rRNA V6 hypervariable region of archaeal DNA was PCR amplified using barcoded primers. The forward primer sequence was 5’CCATCTCATCCCTGCGTGTCTCCGACTCAG-X-AATTGGANTCAACGCCGG-3’, where the first segment comprises the 454 adaptor, X denotes a unique 10-nucleotide barcode sequence for each sample, followed by the 16S archaea F16SA primer sequence. The sequences of two reverse primers (major and minor) were 5’-CCTATCCCCTGTGTGCCTTGGCAGTCTCAG-CGRCGGCCATGCACCWC-3’ and 5’-CCTATCCCCTGTGTGCCTTGGCAGTCTCAG-CGRCRGCCATGYACCWC-3’ , respectively, where the first segment comprises the 454 adaptor and the second segment comprises the 16S archaea 4B-R16SMajor or 4B-R16SMinor primer sequence. Barcoded samples were purified and mixed. Purified PCR products were sequenced at the Australian Genome Research Facility, Brisbane, Australia. Sequence data were separated by barcode into individual samples.
NT22.2 Archaea-August 2009-2
Charles Darwin University
NT22.2 Archaea-August 2009-2