GSM3955014
GSM3955014: RMB392; Mus musculus; RNA-Seq
bones were removed, bone marrow was flushed out, red blood cells were lysed prior antibody staining, progenitor cells were enriched using magnetic beads prior FACS sorting, IFC capture, on-chip lysis & rt-pcr Nextera_XT Kit (Illumina, PN FC-131-1096) with dual indices (Illumina, PN FC-131-1002) was used to prepare single-cell multiplexed libraries Cells were loaded onto C1 integrated fluidic circuits IFC (5- to 10-μm chip) for cell lysis, reverse transcription with oligo (dT) primers and amplification of cDNA on a C1 Single Cell Auto Prep System according to the mRNA-seq protocol of the manufacturer (Fluidigm). Only cells with high-quality cDNA were processed for subsequent library preparation. A Nextera_XT Kit (PN FC-131-1096; Illumina) with dual indices (PN FC-131-1002; Illumina) was used for the preparation of single-cell multiplexed libraries.
GEO
GSM3955014
GEO Accession
GSM3955014