GSM3034757
GSM3034757: Naphthalene-injured single-cell RNA-seq; Mus musculus; RNA-Seq
Surface epithelium was isolated by incubation with Dispase at room temperature for 30 min, followed by manually scraping surface epithelial sheet and incubated with trypsin-EDTA at 37 degree for 30 min. Remaining tissue immediately after removing surface epithelial sheet was dissociated with Collagenase II and Hyauluronidase at 37 degree for 30 min, followed by incubation with trypsin-EDTA at 37 degree for 30 min. Cells were passed through 40-um cell strainer, collected and processed according to 10X genomics single cell protocol. Libraries were prepared using 10X genomics Chromium single cell 3' standard protocol.
GEO
GSM3034757
GEO Accession
GSM3034757