GSM4453090
GSM4453090: Control rep2; Mus musculus; RNA-Seq
CD34-Flt3-Lin-Sca1+cKit+ long-term hematopoietic stem cells (LT-HSCs) were sorted directly into TRIzol Reagent (Thermo Fisher), and RNA was extracted. RNA quality and concentration were assessed using an RNA 6000 bioanalyzer pico kit (Agilent Technologies), and samples with an RNA integrity number more than 8 were used. Complementary DNA was amplied by the SMARTer Ultra Low RNA Kit (Clontech), and sequencing was performed on an Illumina HiSeq with 150 bp paired-end reads
GEO
GSM4453090
GEO Accession
GSM4453090