GSM4292940
GSM4292940: reprogrammed2; Drosophila melanogaster; RNA-Seq
The central nervous systems of larvae were dissected out and underwent RNA purification with Quick-RNA miniprep plus kit (Zymo). Integrity of eluted RNA was analyzed by NanoDrop (Thermo Scientific) and 2100 Agilent Bioanalyzer (Agilent) prior to subsequent experiments. Approximately 1 μg total RNA for each genotype was used. Oligo (dT) magnetic beads were used to select mRNA, then cDNA was synthesized from mRNA fragments by random N6 primers, followed by a second strand cDNA synthesis. After end-repair and adaptor ligation, the double stranded DNA was denatured and circularized on single strand to form the final library.
GEO
GSM4292940
GEO Accession
GSM4292940