GSM3501441
GSM3501441: Zfp36fl/fl Rep2; Mus musculus; RNA-Seq
RNA was isolated from 2 cm of full thickness colonic tissue by pestle homogenization in TRIzol according to the manufacturer's instructions. RNA concentrations were measured on a NanoDrop (ThermoFisher) apparatus. The cDNA libraries were prepared using the NEXTflex™ Rapid RNA Sequencing Kit and NEXTflex™ RNA-Seq Barcodes – 24 Kit (BioO Scientific) as per the manufacturer's instructions. The final product was assessed for its size distribution using Bioanalyzer DNA High Sensitivity Kit (Agilent Technologies) and for its concentration using Kapa library quantification kit (Kapa Biosystems). The samples were sequenced using a HiSeq 2500 that was set to run for 50 cycles using a single-read recipe (TruSeq SBS Kit v3, FC-401-3002) according to the manufacturer's instructions.
GEO
GSM3501441
GEO Accession
GSM3501441