GSM1821427
GSM1821427: E13 GFP-; Mus musculus; RNA-Seq
Total RNA was extracted with Rneasy Plus Mini Kit (QIAGEN) according to manufactureer's protocol. Optical density values of extracted RNA were measured using NanoDrop (Thermo Scientific) to confirm an A260:A280 ratio above 1.9. RIN was confirmed as greater 7 for each sample using Bioanalyzer RNA 6000 Nano Kit (Agilent Technologies). The cDNA libraries were prepared using the NEXTflex™ Rapid RNA Sequencing Kit and NEXTflex™ RNA-Seq Barcodes – 24 Kit (BioO Scientific) as per the manufacturer’s instructions. The final product was assessed for its size distribution using Bioanalyzer DNA High Sensitivity Kit (Agilent Technologies) and for its concentration using Kapa library quantification kit (Kapa Biosystems). Six libraries were pooled per HiSeq lane, followed by on-board cluster generation on a Rapid Run single-end flow cell and subsequent 50 cycles sequencing (v3 sequencing kit) according to the manufacturer's instructions (HiSeq 2500, Illumina).
GEO
GSM1821427
GEO Accession
GSM1821427