E-MTAB-2393:NS107
Illumina HiSeq 2000 paired end sequencing; chick intermediate neural explants
chick intermediate neural explants
107_control_12h
Intermediate neural plate explants were isolated from HH stage 9 embryos and were cultured in collagen drops (SIGMA) with DMEM for indicated time periods. Chick intermediate neural explants were incubated in the F-12 medium (Gibco) for 0 hour (sample 106) or for 12 hours (samples 107 and 110) in the absence (sample 107) or in the presence of 5nM of bFGF (Fibroblast Growth Factor basic) (sample 110). picopure RNA extraction kit Library was constructed by TruSeq RNA sample preparation kit.
MEDICAL RESEARCH COUNCIL, NATIONAL INSTITUTE FOR MEDICAL RESEARCH
E-MTAB-2393:NS107
Medical Research Council, National Institute for Medical Research
E-MTAB-2393:NS107
Experimental Factor: compound
none
Experimental Factor: time
12 hour