Needles_Ex_Bacteria
N_Ex_Bacteria
DNA samples were subject to 454 pyrotag sequencing as in (S E Dowd et al. 2008).  Pyrotag sequencing employed bacterial 16S primers, Gray28F 5‘-GAGTTTGATCNTGGCTCAG (modified from Frias-lopez et al., 2002) and Gray519R 5‘-GTNTTACNGCGGCKGCTG (modified from (Manefield et al., 2002).    All samples were sequenced by a Roche 454 FLX instrument with titanium reagents following manufactures protocols (Roche Inc.). Initial generation of sequence library consisted of a one-step 25 cycle PCR with Hot Start plus Taq polymerase (Qiagen, Inc., Valencia, CA).
Needles_Ex_Bacteria
Harvard University
Needles_Ex_Bacteria