GSM3768368
GSM3768368: WT_TG_5_10x; Mus musculus; ncRNA-Seq
Trigeminal ganglia were harvested and incubated in RNAlater (ThermoFisher) at 4oC overnight.  Tissues were then transferred and stored at -80oC until use.  Tissues were homogenized using a glass dounce homogenizer on ice.  Neuronal nuclei were isolated using rabbit anti-NeuN antibody (Abcam) followed by anti-rabbit IgG microbeads (Miltenyi Biotec) and LS column (Miltenyi Biotec). Libraries construction were performed either with the Drop-seq protocol from the McCarroll lab (for Drop-seq runs) or with method from 10x Genomics for 10x Chromium runs.
GEO
GSM3768368