E-MTAB-2590:wee1-1-0mMHU-3a
Illumina HiSeq 2000 sequencing; Arabidopsis thaliana RNase H2 deficiency counteracts the needs for the WEE1 checkpoint kinase but triggers genome instability
Arabidopsis thaliana RNase H2 deficiency counteracts the needs for the WEE1 checkpoint kinase but triggers genome instability
wee1-1 - 0mM HU - 3
The WEE1 knockout (wee1-1) and the RNH2A/WEE1 double knockout (rnh2a/wee1-1) seeds were germinated on control medium on a nylon mesh. Transferred 5 d after germination to control medium or medium supplemented with 2 mM HU 24h after the transfer 200 root tips (<2 to 3 mm) were collected and frozen in liquid nitrogen. RNA was extracted from root tissue with the RNeasy plant kit (Qiagen). Illumina True-Seq libraries were generated from cDNA according to the manufacture’s protocol and sequenced on a HiSeq2000.
EBI
E-MTAB-2590:wee1-1-0mMHU-3a
European Bioinformatics Institute
E-MTAB-2590:wee1-1-0mMHU-3a
Experimental Factor: genotype
wee1-1
Experimental Factor: compound
none