GSM3188209
GSM3188209: SCR_EBd3_1 [PRO-Seq]; Mus musculus; OTHER
Around  1 million cells were permeabilised and incubated with biotin-labeled NTPs. The RNA was then hydrolyzed with NaOH and biotin containing fragments were enriched by affinity purification using streptavidin-coated magnetic beads. A 3′ sequencing adaptor was ligated to the hydroxyl (OH) group at the 3′ end of nascent RNA, the 5'cap was removed, then a 5'OH was generated by base hydrolysis and converted to 5′ phosphate by treatment with T4 polynucleotide kinase. Then, a 5′ sequencing adaptor was ligated to the nascent RNA. RNA was purified to enrich for fragments with adaptor at both ends and reverse transcribed.
GEO
GSM3188209
GEO Accession
GSM3188209