E-MTAB-1654:D6s4B5_1N+r1
OMalley_et_al2013
OMalley_et_al2013
D6s4B5 1N+r1
MEFs and ES Cells were cultured in GMEM + 100Units LIF. Intermediate reprogramming samples were cultured in the presence of dox, VitC and Alk5i for 10 or 15 days. 20,000 to 200,000 cells from each sample were sorted by flow cytometry. Pelleted cells were resuspended in 500uL TRI Reagent (Invitrogen). RNA was extracted in the usual manner via phenol-chloroform extraction. RNA quality was verified using a Agilent Bioanalyzer. RNA was diluted to a concentration of 10ng/uL in 10mM TRIS ph7.6, 0.05% Tween-20 buffer. Library was constructed as published in Islam et al. Genome Research, 21, 1160-1167, doi:10.1101/gr.110882.110 (2011).
University of Edinburgh
E-MTAB-1654:D6s4B5_1N+r1
Experimental Factor: individual
D6s4B5 1N+
Experimental Factor: cell type
intermediate reprogramming population