sa79
Metschnikowia fructicola 277 wild type
Total RNA from frozen yeast pellet was extracted using MasterPureTM Yeast RNA Purification Kit (Epicentre® Biotechnologies, Madison, WI, USA) according to the manufacturer`s instructions.  Quality and concentration of total RNA was analyzed by gel electrophoresis and a spectrophotometer ND-1000, respectively (NanoDrop, Wilmington, DE, USA). First-strand cDNA was synthesized with Verso cDNA Kit (Thermo Fisher Scientific, Epson, UK), from 1 µg of total RNA that had been pretreated with 1.5 units of RNase I (Epicentre® Biotechnologies, Madison, WI, USA).
sa79
ARO
sa79