G. polyphemus SD103 - immunome sequencing
G. polyphemus SD103 - immunome sequencing
We created Illumina adaptor-ligated libraries using Agilent SureSelect XT2 Reagent Kits for the Illumina MiSeq (Agilent Technologies, Santa Clara, CA, USA), pooled 16 prepared libraries, and used MYcroarray (Ann Arbor, MI, USA) hybridization and capture reagents and protocols for sequence capture. We then sequenced post-capture amplification libraries on two Illumina MiSeq sequencer flow cells (i.e., all individuals sequenced twice) using 75-bp paired-end reads.
SD103
Louisiana State University
G. polyphemus SD103 - immunome sequencing