GSM3712258
GSM3712258: PakiT03 - IFN - IRF1 - 9h; Pteropus alecto; RNA-Seq
Cells were lysed into TRK lysis buffer supplemented with β-ME, freeze-thawed, homogenised and extracted as per manufacturers protocol (OMEGA EZNA total RNA Kit I). Total RNA was checked using the RNA 6000 LabChip Kit on the Agilent Bioanalyzer (Agilent Technologies, Palo Alto, CA). RNAseq libraries were prepared using Illumina Tru-Seq Stranded Total RNA with Ribo-Zero Gold kit following the manufacturer's instructions (Illumina, San Diego, California, USA). Libraries were validated with an Agilent Bioanalyzer (Agilent Technologies, Palo Alto, CA), diluted and applied to an Illumina flow cell using the Illumina cBOT system. Sequencing was performed on an Illumina HiSeq 3000 sequencer at the Duke-NUS Genome Biology Facility with the paired-end 150-bp read option.
GEO
GSM3712258
GEO Accession
GSM3712258