GSM2939181
GSM2939181: Library 6026; Mus musculus; RNA-Seq
Cells were lysed in RLT buffer containing 1% BME. RNA extraction was performed using Qiagen RNeasy kits. Total RNA was used as template for the initial reverse transcription, which included 0.27 uM of barcoded primer, containing a T7 promoter sequence. 48-96 cDNA samples were pooled, followed by a 8 h T7 amplification. 50 ng of the resulting aRNA was used as input for library generation using the Quantseq aRNA kit (Lexogen, Cat #043.24.V0.1).
GEO
GSM2939181
GEO Accession
GSM2939181