GSM3186802
GSM3186802: AC2siRNA_2; Homo sapiens; RNA-Seq
Total RNA from three biological replicates of non-target siRNA and AC093323.3 siRNA treated H460 cells were extracted using the RNeasy mini kit (QIAGEN, 74104) and sent for PolyA sequencing. Indexed PolyA libraries were prepared using 200ng of total RNA and 14 cycles of amplification with the Agilent SureSelect Strand Specific RNA Library Prep Kit for Illumina Sequencing (Agilent, Cat No: G9691A). Libraries were quantified by qPCR using a Kapa Library Quantification Kit for Illumina sequencing platforms (Kapa Biosystems Inc., Cat No: KK4835). Paired-end 75bp sequencing was carried out by clustering 1.9 pM of the pooled libraries on a NextSeq 500 sequencer (Illumina Inc.).
GEO
GSM3186802
GEO Accession
GSM3186802