GSM4200573
GSM4200573: 14d Input DNA biological repeat 1; Saccharomyces cerevisiae; ChIP-Seq
Cells were lysed with 300 ml acid washed glass beads (425-600 mm) by vigorous agitation for 30 s in a BeadRuptor2 followed by 30 s on ice, repeated 7 times. Sufficient lysis (90%) was confirmed by light microscopy. The lysate was subsequently sonicated at 4°C to obtain an average DNA fragment length of 300 bp. Samples were centrifuged and the supernatant, containing sheared chromatin, recovered. Libraries were prepared for sequencing using standard Illumina protocols
GEO
GSM4200573
GEO Accession
GSM4200573