GSM4088792
GSM4088792: infected - library 2; Homo sapiens; RNA-Seq
Cell capture and library preparation were performed as described using a modified version of inDrops protocols(Klein et al., 2015; Plasschaert et al., 2018). Prior to library preparation, RNA fractions generated from each population were split in half, with one half being used for standard library prep and the other half for targeted lineage barcode enrichment. To enrich for lineage barcodes, library preparation was modified as previously described (Weinreb et al., 2019) by skipping RNA fragmentation, priming the RT reaction using a barcode-specific primer (TGAGCAAAGACCCCAACGAG), introducing an extra PCR step using a targeted primer (8 cycles using Phusion 2X master mix; Thermofisher; primer sequence = TCG TCG GCA GCG TCA GAT GTG TAT AAG AGA CAG NNN Ntaa ccg ttg cta gga gag acc atat), and 1.2X bead purification (Agencourt AMPure XP). All targeted and non-targeted final libraries were pooled at equimolar ratios and sequenced using Illumina NextSeq 500 Sequencing (75 Cycles, 75bp Single Read sequencing).
GEO
GSM4088792
GEO Accession
GSM4088792