HpGf4
RNA-Seq of Heligmosomoides polygyrus: germ-free mice, replicate 4
For transcriptome sequencing on an Illumina platform a TruSeq RNA library generation was utilized. The library was generated by using the TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego, CA, USA) following the manufacturers instructions. The library was quantified by using the KAPA Library Quantification Kit for Illumina (Kapa Biosystems, Wilmington, MA, USA). The library size was determined by using the High Sensitivity DNA Analysis Kit for the 2100 Bioanalyzer Instrument (Agilent Technologies, Waldbronn, Germany). Libraries were adjusted to a concentration of 12 pM and sequenced on a HiSeq 1500 instrument (Illumina, San Diego, CA, USA) in rapid mode. For cluster generation the TruSeq Rapid PE Cluster Kit v2 was used. Cluster generation was performed on board. For sequencing the HiSeq Rapid SBS Kit v2] was used to sequence 100 + 100 bases.
HpGf4
SUB4401819
HpGf4