GSM3185945
GSM3185945: CD49f+ HSC Subset2 SLX.15682.i719_i522; Homo sapiens; RNA-Seq
CD49f+ HSCs were purified by FACS from CD34+ cord blood samples obtained as in Notta et al., Science 2011. Novel HSC subsets were defined as follows:  CD49f+ Subset2 cells (CD19- CD38- CD45RA- CD90+ CD49f+ CD34hi CLEC9Alo),  HSC/MPP Subset2 (CD19- CD38- CD45RA- CD34hi CLEC9Alo). Myelo-lymphoid restricted progenitors were defined as follows: LMPP (CD19- CD38- CD34+ CD45RA+ CD10-), MLP (CD19- CD38- CD34+ CD45RA+ CD10+). Single cells were directly sorted into lysis buffer according to the Smart-seq2 protocol published by Picelli et al., Nat. Protocol 2014. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO
GSM3185945
GEO Accession
GSM3185945