GSM4368198
GSM4368198: Rosettes, Mannitol_Heat_3; Arabidopsis thaliana; RNA-Seq
Rosettes were harvested and immediately frozen in liquid nitrogen. Tissue was ground into fine powder using liquid nitrogen, and then kept at -80°C until use. Total RNA was extracted using the Promega Kit (SV Total RNA Isolation System, spin protocol). The integrity of the extracted RNA was anaylsed by gel electrophoresis and its concentration was measured using a NanoDrop (ND-1000) spectrophotometer. Libraries were prepared using the TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego, CA, United States) and quantified with a Qubit 2.0 (Invitrogen, Waltham, MA, United States). Samples were multiplexed with 12 libraries per lane and sequenced in paired-end mode (Rapid Run, 100 bp read length) on an Illumina HiSeq 2500 platform.
GEO
GSM4368198
GEO Accession
GSM4368198