M1712
DNAseq of Paramecium bursaria Dd1:Whole cell
The aposymbiotic strain of Dd1 in the early stationary phase was starved for 2 days and collected by water pipe equipped with 11m pore size nylon membrane (NY1102500, Millipore). The cells were washed with modified Dryls solution twice and lysed by equal volume of 0.25 M TCMS buffer (10 mM Tris-HCl pH 8.0, 2 mM CaCl2, 8 mM MgCl2, 0.25 M sucrose) with 0.3% (w/v) NP-40. The DNA was extracted by the QIAGEN Genomic-tip 20/G (Cat No.10223, QIAGEN).
M1712
SUB6042154
M1712