Root metagenome sample
Root metagenome sample
Library preparation was performed at Genotypic Technology’s Genomics facility. 25 ng of Nanodrop quantified DNA for sample was used for amplifying ITS region of 18S with specific primers which also have a ‘tag’ sequence that are complementary to Illumina sequence adapter and index primers from the Nextera XT Index kit V2. This round of PCR generates single Amplicon of ~280-300 bp. In the next round of PCR (indexing PCR) Illumina sequencing adapters and dual Indexing barcodes are added using limited cycle PCR to give a final product of ~350-400bp. The library was cleaned using HighPrep PCR (Magbio, Cat # AC-60050) magnetic beads and was Qubit quantified (Refer Table 1.) and validated for quality by running an aliquot on High Sensitivity Bioanalyzer Chip (Agilent).
CSIR Central institute of medicinal and aromatic p
Root metagenome sample