E-MTAB-6958:LK2_BCL11A-ContSh_Input_p
Illumina HiSeq 2500 paired end sequencing; ChIP-Seq of BCL11A and SOX2 in lung squamous cell carcinoma (LUSC) LK-2 cell line with or without BCL11A knockdown
ChIP-Seq of BCL11A and SOX2 in lung squamous cell carcinoma (LUSC) LK-2 cell line with or without BCL11A knockdown
LK2_BCL11A-ContSh_Input_p
LK-2 cells were cultured in RPMI (gibco) supplemented with 10% fetal bovine serum and 1% Penicillin/Streptomycin.    ChIP-Seq was done exactly as described in (Schmidt, D., et al. ChIP-seq: Using high-throughput sequencing to discover protein–DNA interactions. Methods 48, 240-248 (2009)). Briefly 2 x 15cm plates per cell line were formaldehyde crosslinked, nuclear fraction was isolated and chromatin sonicated using Bioruptor Pico (Diagenode). Libraries were produced at the Wellcome Trust Sanger Institute sequencing facility. Each library was split into two and run on separate lanes
UNIVERSITY OF CAMBRIDGE
E-MTAB-6958:LK2_BCL11A-ContSh_Input_p
Experimental Factor: RNA interference
scrambled shRNA
Experimental Factor: immunoprecipitate
input DNA