Bgl3_Lib1_sort3
DMS of Bgl3 from Streptomyces, Library1,  sort3
A GH1 gene library was grown in a liquid  culture and active enzyme variants were isolated via microfluidic screening.  The plasmids for the active GH1 variants was extracted, the GH1 gene insert  was digested out of the expression vector, and isolated via gel extraction.  The GH1 gene insert was then randomly fragmented using the Nextera XT kit and  sequenced using a 2x300 MiSeq v3 kit.
Bgl3_Lib1_sort3
SUB7240601
Bgl3_Lib1_sort3