OA6_D5_M4
Ocean acidification mesocosms 16s rRNA library
We extracted DNA using the Gentra Puregene Yeast/Bact. Kit (Qiagen) according to the manufacturers instructions supplemented with one round of bead beating (60 seconds).  Microbial communities were characterized using a dual index approach with the following portions of the primer targeting the V4/V5 region of the bacterial and archaeal 16S rRNA gene: 515F-Y (5-GTGYCAGCMGCCGCGG TAA) and 926R (5-CCGYCAATTYMTTTRAGTTT).
OA6_D5_M4
SUB5735519
OA6_D5_M4