GSM4368368
GSM4368368: S_502_0; Mus musculus; RNA-Seq
Hipocampus was dissected out of the murine brains. For RNA extraction, Qiagen's miRNEasy Mini kit was used according to manufactors protocol RNA-seq libraries were prepared with the TruSeq Stranded mRNA LT sample preparation kit (Illumina) using Sciclone and Zephyr liquid handling robotics (PerkinElmer). Library amounts were quantified using the Qubit 2.0 Fluorometric Quantitation system (Life Technologies) and the size distribution was assessed using the Experion Automated Electrophoresis System (Bio-Rad). For sequencing, libraries were pooled, diluted and sequenced on an Illumina HiSeq 3000 instrument using 50 bp single-read chemistry.
GEO
GSM4368368
GEO Accession
GSM4368368