Chesson_Ao  10X single-cell RNAseq
Chesson_Ao  10X single-cell RNAseq
One mL of fresh venous blood was obtained from each monkey and centrifuged at 2,500 rpm for three minutes to separate and remove the serum. Blood cells were transferred to 50 mL tubes containing RPMI 1640 to a final 0.5  1.0% hematocrit solution. Parasitized cells were enriched using MACS LS separation columns. We determined the number and viability of the purified cells from each sample using a BioRad TC20 Automated Cell Counter and loaded, from each sample, an estimated ~3,000 cells on a 10X Genomics Chromium controller to prepare a barcoded 3-end scRNA-seq library according to the manufacturers instructions. For each of the 10 libraries, we minimized the amount of processing time so that less than three hours elapsed between the blood collection and the cell isolation on the Chromium controller. The ten scRNA-seq libraries were sequenced with an Illumina HiSeq 4000 to generate paired-end reads of 75 bp. (One library was sequenced using 101 bp paired-end reads but was trimmed, before alignment, to 75 bp for consistency).
Chesson_Ao  10X single-cell RNAseq
SUB6912263
Chesson_Ao  10X single-cell RNAseq