GSM3768822
GSM3768822: KO2; Mus musculus; RNA-Seq
Embryonic lenses were dissected in ice-cold PBS. The tissues were stabillized for RNA degradation by overnight incubation ay 4°C in RNAlater solution (Qiagen). Next day the solution was removed and the tissues were stored at -80°C. mRNA was extracted using RNeasy Micro Kit (Qiagen) and the quality and quantity of the RNA were analyzed by Bioanalyzer and Qubit. NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina was used for sequencing library preparation of 500ng of mRNA. RNA libraries were prepared using standard Illumina protocols for polyA selected and stranded libraries
GEO
GSM3768822
GEO Accession
GSM3768822