GSM4706025
GSM4706025: M2; Mus musculus; RNA-Seq
Neural retinas were dissected out, retinal cells enzymatically dissociated and sorted by FACS, eGFP+ cells (cones) were collected, lysed and stored in the lysis buffer of Nucleopsin RNA XS kit. 100 ng RNA per sample was used for RNA-seq library was constructed using NEBNext® Ultra™ Directional RNA Library Prep Kit for Ilumina with NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs) according to manufacturer's protocol. The libraries were quantified using Agilent 2100 Bioanalyzer (Agilent) and Quibit quantification. RNA-seq libraries were sequenced using NextSeq550 PE150 (Illumina).
GEO
GSM4706025
GEO Accession
GSM4706025