GSM3033406
GSM3033406: 2h_Mock_D2_281104; Homo sapiens; RNA-Seq
5'000 live cells were sorted in eppendorf containig 10uL of PBS. RNA was extracted with Qiagen RNeasy Micro kit and 1 uL of RNA was added to 4uL of lysis buffer (0.4% Triton, 2U/uL RNase inhibitor (Clonotech, Takara), 7.5uM oligo dT (Biomers) and 10mM dNTP [supplemented with ERCC spike-in 1:10,000,000] (Invitrogen)) and processed as for single cells. Nextera XT DNA sample preparation kit with the dual index strategy
GEO
GSM3033406
GEO Accession
GSM3033406