F1_cross_Nvitripennis_to_Ngiraluti
RNA-Seq of the venom gland and reservoir of a F1 cross of N. vitripennis female to N. giraluti male
Venom transcriptomes were generated for each species by pooling 50 venom apparatus (venom gland + reservoir). Following dissections, samples were immediately collected into 40ul of 4oC TRIzol Reagent¨ (Ambion) in 1.5ul microcentrifuge tubes and stored at -80oC. Total RNA extractions were performed as per manufacturerŐs protocol, followed by quantification and quality checking using Agilent 2100 Bioanalyzer. TruSeq mRNA (Illumina) library construction and 100bp paired-end sequencing on Illumina HiSeq 2500 platform were performed by University of Rochester Genomics Research Center (URGRC). cDNA for each species was indexed with a unique adapter. Each library was normalized by equimolar multiplexing before sequencing at ~ 1 library/10th of a lane.
F1_cross_Nvitripennis_to_Ngiraluti
SUB2027496
F1_cross_Nvitripennis_to_Ngiraluti