GSM3503260
GSM3503260: IU1781 WT+TEX-2; Streptococcus pneumoniae D39; ncRNA-Seq
RNA was isolated from cells via the Fastprep blue kit (MPBiomedicals) For samples 1-3, the library was generated by removing DNA by DNase treatment, depleting rRNA with Ribo-Zero rRNA removal kit for gram-positive bacteria, fragmenting RNA reagent (Ambion), removing 5' triphosphates via RNA 5' polyphosphatase, and subsequently utilizing the TruSeq Small RNA library kit.  For samples 4-6, the same protocol was used as for Samples 1-3, but prior to RNA 5' polyphosphatase treatment the RNA was treated with Terminator 5'-phosphate-dependent exoribonuclease.
GEO
GSM3503260
GEO Accession
GSM3503260