GSM2937066
GSM2937066: nasal control indiv 2; Homo sapiens; RNA-Seq
Nasal epithelial cells were collected from behind the inferior turbinate with a cytology brush using a nasal illuminator. Whole blood was collected using PAXgene RNA blood tubes. RNA was isolated using PAXgene RNA blood extraction kits. Poly(A) selected RNA-seq libraries (n=38) were constructed using 500 ng of blood and nasal airway epithelial total RNA from 9 atopic asthmatics and 10 non-atopic controls. Libraries were constructed and barcoded with the Illumina TruSeq RNA Sample Preparation v2 protocol. Barcoded nasal airway RNA-seq libraries from each of the 19 subjects were pooled and sequenced as 2 x 100bp paired-end reads across two flow cells of an Illumina HiSeq 2000. Barcoded blood RNA-seq libraries from each of the 19 subjects were pooled and sequenced as 2 x 100bp paired end reads across 4 lanes of an Illumina Hiseq 2000 flow cell.
GEO
GSM2937066
GEO Accession
GSM2937066