GSM3446020
GSM3446020: WT bone replicate 3; Mus musculus; RNA-Seq
Laser Capture Microdissection was used to isolate tissues from Meckels cartilage, mandibular bone and mandibular condylar cartilage, respectively. RNA was isolated with Arcturus Picopure RNA Isolation Kit as described by the manufacturer and eluted in a final volume of approximately 16 µl of elution buffer. RNA quality and concentration was assessed using the Agilent RNA Pico kit and Agilent Bioanalyzer 2100 as described by the manufacturer. Libraries were prepared with NuGEN Ovation RNA-Seq System v2 (amplification) and Illumina Nextera XT Library Prep kit. Fragments were sequenced using paired end reads (2×100 bp) on the Illumina HiSeq platform.
GEO
GSM3446020
GEO Accession
GSM3446020