GSM3446045
GSM3446045: wild-type retina_control AAV viral vector (AAV2/8.CMV.miR204MUT), biol rep1 (animal n. 1, Right Eye OD); Mus musculus; RNA-Seq
Extraction of total RNA was performed according to the manufacturer's instructions (miRNeasy kit (Qiagen, Inc., Hilden, Germany). Libraries were prepared according manufacturer's instructions (TruSeq RNA Sample Preparation kit, Illumina) starting from 4 μg of total RNA. Quality control of library templates was performed using a High Sensitivity DNA Assay kit (Agilent Technologies) on a Bioanalyzer (Agilent Technologies). The Qubit quantification platform (Qubit 2.0 Fluorometer, Life Technologies) was used to normalize samples for the library preparation. The amplified fragmented cDNA of ~200 bp in size were sequenced in paired-end mode using the HiSeq 1000 (Illumina) with a read length of 2×100 bp
GEO
GSM3446045
GEO Accession
GSM3446045