BD02T6
Gulf of Mexico seawater enriched with Macondo oil and dispersant for 6 days
Natural seawater was collected on July 27, 2013, at the depth of 1200 - 1300 m, approximately between 1 to 5 km to the Deepwater Horizon wellhead.  Macondo oil and Corexit 9500 A (Nalco, Sugar land, TX) were used to generate oil droplet of 10 μm  in median diameter. Oil droplet were mixed with seawater, incubated in a rotation carousel system at 4 oC, in the dark, for 6, 18, 64 days. Genomic DNA was extracted from filters. DNA (200ng) was sheared using a Covaris instrument. The library was constructed using Illumina TruSeq nano DNA kit
BD02T6
Lawrence Berkeley National Lab
BD02T6