GSM4512702
GSM4512702: csf1r_doubleknockout2_28hpf; Danio rerio; RNA-Seq
35 embryos (28 hpf wt, 50 hpf wt, 28 hpf csf1rdm) or 70 embryos (csf1rdm 50 hpf) were pooled per replicate sample and dissociated with trypsin on ice. mpeg-GFP+ cells were FAC-sorted directly into Trizol reagent. RNA was harvested using Trizol reagent. cDNA amplification: SMART-seq V4 ultra low input RNA kit for sequencing (Takara BIO USA, Inc.). Illumina Truseq Sample Preparation V.2 guide was used and the samples were paired end-sequenced (2x75 bp) on the Illumina HiSeq 2500. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO
GSM4512702
GEO Accession
GSM4512702