GSM3400701
GSM3400701: WT-Tg HFD [WAT-TgWt_m2_s14]; Mus musculus; RNA-Seq
Animals were euthanized under Schedule 1 of the Animals (Scientific Procedures) Act 1986. Subcutaneous white adipose was retrieved from the inguinal region near the flank, the lymph node removed and the tissue flash-frozen in liquid nitrogen. RNA extraction was performed using Trizol Reagent and isolated using Qiagen RNeasy Mini Kits as per manufacturers instructions. Total RNA quantification was performed using a NanoDrop spectrophotometer. RNA integrity was assessed, with a minimum RIN of 7 permitted for sequencing (Maryam Clausen, contributer). Illumina TruSeq stranded mRNA-seq protocol
GEO
GSM3400701
GEO Accession
GSM3400701