GSM4200599
GSM4200599: Macrophages exposed to cartilage fragments 3; Mus musculus; RNA-Seq
Differentiated macrophages cultured with or without cartilage fragments were lysed with TRIzol Reagent and harvested for RNA purification. RNA was purified using RNeasy Mini kit columns according to the manufacturer's instructions, and integrity of samples was assessed by determining 28S/18S ribosomal RNA bands with an Agilent 2100 bioanalyzer. High-quality DNA-free RNA with integrity score > 9.0 was used to generate libraries using the TruSeq Stranded mRNA Sample Preparation Kit(Illumina, San Diego, USA) and their quality was examined by Bioanalyzer High sensitivity DNA kit (Agilent Technologies). The paired-end reads (100 bp) were further obtained by Illumina HiSeq 2500 (Illumina)
GEO
GSM4200599
GEO Accession
GSM4200599