MP120N
Normal
Total RNA was extracted using Trizol Reagent (Invitrogen) according to manufactures instructions, from freshly frozen samples stored at -80C. The RNA was suspended in RNase-free water, and purified with an RNeasy MinEluteTM clean up kit (Qiagen). RNA concentration and integrity was assessed using a Nanodrop 1000 spectrophotometer (Thermo Scientific, Wilmington, Delaware) and Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, California), according to manufacturers protocol. Seventeen samples were submitted to the Van Andel Research Institute (VARI) Genomics Core for 2 x 75 bp RNA sequencing on an Illumina NextSeq 500. Libraries were prepared using a Kapa RNA HyperPrep Kit with ribosomal reduction, pooled, and sequenced across two flowcells to yield approximately 50-60 million reads/sample.
MP120N
SUB4681589
MP120N