E-MTAB-5191:THP1 BR1 PMA_RNA_p
Illumina HiSeq 2500 paired end sequencing; RNA-seq analysis of cell-line models for monocyte to macrophage transformation: Project 3 of Open Targets - Epigenomes of Cell Lines
RNA-seq analysis of cell-line models for monocyte to macrophage transformation: Project 3 of Open Targets - Epigenomes of Cell Lines
THP1 BR1 PMA_RNA_p
2 nM PMA at 0 hrs, control medium at 24 hrs and harvesting of cells at 42 hrs. Qiagen RNeasy plus mini kit Total RNA from each sample was used as input for the Illumina TruSeq Stranded messenger RNA LT Sample Prep Kit (Illumina) and sequencing libraries were created according to the manufacturer’s protocol. Briefly, poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads. Following purification, the mRNA was fragmented and copied into first strand complementary DNA using random primers and reverse transcriptase. Second strand cDNA synthesis was then done using DNA polymerase I, RNase H and substituting dUTP for dTTP. The cDNA was ligated to adapters and enriched with PCR to create the final cDNA library. The library was pooled and sequenced
Target Sciences group, Glaxo Smithkline
E-MTAB-5191:THP1 BR1 PMA_RNA_p
Experimental Factor: phorbol myristate acetate only
growth condition