92.1_shMITF_c6_DOX_24hr_R1_RNA
RNAseq of 92-1 human uveal melanoma cell line with shRNA knockdown of MITF at 24 hours
200 ng RNA was used as input to the Illumina TruSeq Stranded mRNA Library Prep Kit, High Throughput (Cat# RS-122-2103), and the sample libraries were generated per manufacturers specifications on the Hamilton STAR robotics platform. The PCR amplified RNA-Seq library products were then quantified using the Advanced Analytical Fragment Analyzer Standard Sensitivity NGS Fragment Analysis Kit (Cat# DNF-473). The samples were diluted to 10 nM in Qiagen Elution Buffer (Qiagen material # 1014609), denatured, and loaded at a range of 2.5 to 4.0 picomolar on an Illumina cBOT using the HiSeq 4000 PE Cluster Kit (Cat# PE-410-1001). The RNA-Seq libraries were sequenced on a HiSeq 4000 at 75 base pair paired end with 8 base pair dual indexes using the HiSeq 4000 SBS Kit, 150 cycles (Cat# FC-410-1002). The sequence intensity files were generated on instrument using the Illumina Real Time Analysis software. The resulting intensity files were demultiplexed with the bcl2fastq2 software and aligned to the human transcriptome using PISCES version 2018.04.01.
92.1_shMITF_c6_DOX_24hr_R1_RNA
SUB7224968
92.1_shMITF_c6_DOX_24hr_R1_RNA