Tel
Whole genome sequence of Tel, a telomere elongation mutant of Drosophila melanogaster
Genome sequencing was done on an Illumina GA IIx sequencer. Five micrograms of genomic DNA was sheared to ~400 bp using a Covaris E210 with settings: duty cycle 20%, intensity 4, cycles 200.  The DNA was size-selected for the range 300-600 bp on a 2% agarose gel and extracted using a Gel Extraction Kit (Qiagen). The library was constructed using NEB Next DNA Library Prep Master Mix Set for Illumina (New England Biolabs) and Illumina’s Paired-End DNA Sample Prep Oligo Only Kit.  The resulting library was selected on a 2% agarose gel for 440-560 bp and extracted using a Gel Extraction Kit (Qiagen).  To insure that the library was not over-amplified a test amplification was performed in which aliquots of a PCR reaction were removed every 2 cycles from 4-16.  These aliquots were evaluated on a 2% agarose gel and an optimal cycle number was selected for subsequent large scale amplification in which unique barcodes were added to each library. Eight cycles were selected for these libraries.  The amplification reaction was cleaned using two rounds of AMPure XP Beads (Agencourt).  Each library was run in one lane of a GAIIx to generate paired-end, 101 base reads.  Data was processed using RTA1.6.32.0 and GERALD 1.15.  For Tel 66,654,840 reads were obtained and for y w 76,757,736 reads, representing genome coverage of 48X and 55X, respectively.
Tel
NIEHS
Tel