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RNA-Seq of androgenic gland of mantis shrimp
High-quality RNA samples from O. oratoria were sent to Biomarker Technologies Corporation (Beijing, China) for cDNA libraries construction and sequencing. mRNA was purified by the interaction of the poly (A) tails and magnetic oligo (dT) beads. RNA sequencing libraries were generated using the TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA) with multiplexing primers, according to the manufacturerĄŻs protocol. The cDNA library was constructed with average inserts of 250 bp (150~250 bp), with non-stranded library preparation. The cDNA was purified using a QiaQuick PCR extraction kit (Qiagen, Inc., Hilden, Germany). The short cDNA fragments were subjected to end repair, adapter ligation, and agarose gel electrophoresis filtration. Then, the suitable fragments were selected as templates for PCR amplification.
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SUB2839409
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