180301_NB551164_0024_AHJFGTBGX5
GBS data of walleye: fin clip
100 ng of DNA was digested with 10 units BamHI and NsiI (New England Biolabs; NEB) enzyme combination and incubated at 37 C for 2 h. Following digestion, samples were then ligated with 200 units of T4 ligase (NEB) and phased adaptors at 22 C for 2 h to inactivate the T4 ligase. The ligated samples were purified with SPRI beads and then amplified for 18 cycles with 2 NEB Taq Master Mix along with sample-specific barcodes. Libraries of walleye samples were purified, quantified, pooled and size selected for 300 to 744bp fragments and diluted to 1.7 pm for sequencing. The pooled libraries were loaded across four lanes of a 150bp singleread sequencing on an Illumina NextSeq 550.
180301_NB551164_0024_AHJFGTBGX5
SUB6035740
180301_NB551164_0024_AHJFGTBGX5