GSM1155653
GSM1155653: wildtype rep2; Drosophila melanogaster; RNA-Seq
Small RNA libraries from the cn bw control line, and from cuffwm25 homozygous ovaries were produced with the Small RNA Library Prep Kit (Illumina). Briefly, 20 μg total RNA extracted from the different genotypes was separated on a 15% denaturing polyacrylamide/urea gel electrophoresis (Invitrogen) for 1 h at 200 V. A gel slice corresponding to 18–30-nt-long RNAs was isolated and small RNAs were eluted and processed with the Small RNA Library Prep Kit as per the manufacturer's instructions. Small RNA libraries were subsequently sequenced on the Solexa Genome Analyzer II platform.
GEO
GSM1155653
GEO Accession
GSM1155653