GSM4143297
GSM4143297: Isotype_control; Toxoplasma gondii; OTHER
Parasite nuclei were isolated and bound to Concanalin A coated magnetic beads, and incubated with either anti-Ty (BB2) antibody or an isotype control. pAG-Mnase was then added and incubated to allow for antibody-targeted cleavage of DNA into  fragments. These fragments were allowed to diffuse out of the nucleus, and extracted by phenol-chloroform-isoamyl alcohol followed by ethanol precipitation. Following end-repair, phosphorylation, and polyA-tailing, fragments were ligated with TruSeq Illumina indexed adapters and further amplified by PCR.
GEO
GSM4143297
GEO Accession
GSM4143297