GSM4513767
GSM4513767: Saline 1.1; Rattus norvegicus; RNA-Seq
RNA was extracted using the Qiagen AllPrep kit. Briefly, 17-75mg of tissue were homogenized in 600-2,400ul RLT buffer, and then processed through the AllPrep kit following manufacturer's instructions. RNA samples were then DNase treated using the TurboDNA-free kit (Thermo Fisher Scientific) and cleaned with the Zymo RNA Clean and Concentrator kit. 250ng of RNA underwent cDNA library preparation using the Illumina TruSeq Stranded mRNA kit, followed by cluster generation and sequencing (2x100bp paired end read) on the Illumina HiSeq 2500.
GEO
GSM4513767
GEO Accession
GSM4513767