Day  0_F8_Hemophilic arthropathy_Acute_3h_Injured_1 (Right Joint-1)
Small  RNA profiling of joint tissue in acute hemophilic arthropathy murine model-3
Small RNA sequencing libraries were prepared with NEBNext Multiplex small RNA library preparation kit. 100 ng of total RNA was used as starting material. 3' SR adaptors were ligated to the specific 3-OH group of micro RNAs followed by 5' SR adaptor ligation. Ligated products were reverse transcribed, enriched and barcoded by PCR (15 cycles). Fragments were selected using 4% Agarose E-gels. Finally, the sequencing library was quantified by quantitative PCR using Qubit High Sensitivity Assay (Invitrogen). The obtained libraries were pooled, cluster amplified on Illumina Flow cell and sequenced on an Illumina HiSeq 2500 instrument to generate 20 M, 50bp single-end reads.
Day  0_F8_Hemophilic arthropathy_Acute_3h_Injured_1 (Right Joint-1)
SUB6040366
Day  0_F8_Hemophilic arthropathy_Acute_3h_Injured_1 (Right Joint-1)