Swine respiratory tract mycoplasmas transcriptome
Mycoplasma hyopneumoniae 7448 transcriptome
cDNA library preparation and pyrosequencing was performed using GS-FLX Titanium series reagents essentially following the manufacturer’s instructions (Roche Diagnostics, Germany). An amount of 200 ng of mRNA was used for synthesing the first and second strand of cDNA according to the cDNA Rapid Library Preparation Method Manual GS FLX Titanium Series (Roche Diagnostics, Germany). After library construction, the samples were quantified using a QubitTM system (Invitrogen, USA), and average fragment sizes were determined by Agilent 2100 Bioanalyzer (Agilent, USA). Three cDNA libraries were generated and sequenced in a Roche/454 GS-FLX system.
Mycoplasma hyopneumoniae 7448
National Laboratory of Scientific Computation
Swine respiratory tract mycoplasmas transcriptome