E113
Experiment 113
We extracted DNA with a Bacteremia DNA isolation kit (MoBio Laboratories, Carlsbad, CA) following the manufacturer's instructions. The DNA extracts and 9 negative controls were subjected to V1-V3 region 16S rRNA PCR, following Hawlena et al. (2013), and 454 pyrosequencing was performed on a Roche/454 GS-FLX Titanium system.
V1-V3 region 16S rRNA PCR
University of North Texas
E113