GSM2935025
GSM2935025: CTL_24_lineB_batch4; Homo sapiens; RNA-Seq
Total RNA was isolated using the RNeasy plus isolation kit (Qiagen, Valencia, CA) according to the manufacturer's protocol.  An Agilent Bioanalzyer (Agilent Technologies, Santa Clara, CA) was used to assess RNA quality and quantity. Polyadenylated RNA was purified and used to prepare complementary DNA libraries for high throughput sequencing using the Illumina mRNA-Seq kit according to the manufacturer's protocol (Illumina, San Diego, CA). Sequencing of fragmented complementary cDNA was performed using the Illumina Genome Analyzer Iix
GEO
GSM2935025
GEO Accession
GSM2935025