GSM3576392
GSM3576392: RNA.P14.hep.Wt.sexF.2; Mus musculus; RNA-Seq
Hepatocytes were resuspended in TRIzol (Thermo Fischer 15596026) and stored at -20°C until the day of library preparation, when RNA was isolated from TRIzol per the manufacturer's protocol and brought through at least cDNA synthesis all on the same day. RNA isolated from TRIzol was resuspended in TE. Next, total RNA was polyA-selected using 50 μL Invitrogen Dynabeads (dT)25-61002 per the manufacturer's protocol.  Libraries were prepped from polyA+ RNA using the NEBNext mRNA librarpy prep master mix set for Illumina (NEB E6110) and sequenced with a  NEBNext 500/550 High Output Reagent Cartridge V2 75 cycles (Ref 15057934) and loaded onto the NextSeq 500 for 75bp single-end sequencing. NEBNext mRNA Library Prep Master Mix Set for Illumina E6110
GEO
GSM3576392
GEO Accession
GSM3576392