R2_T1
Time point 1, repeat 2
E. coli cells carrying the EcoWG1 library on plasmid psgRNA were grown on LB agar plates with aTc to induce the expression of dCas9. The plasmid was then extracted, the library amplified by PCR and sequenced. Time point 1 (3H) of the 2nd biological replicate
R2_T1
SUB6549633
R2_T1