E-MTAB-7395:Sample 9_p
Illumina HiSeq 4000 paired end sequencing; Experimental overactivity or blockade of transforming growth factor-B each generate a specific ureter malformation
Experimental overactivity or blockade of transforming growth factor-B each generate a specific ureter malformation
Sample 9_p
Mouse ureters were extracted from embryonic stage 15 mice and then cultured in serum-free defined media according to an established protocol (PMID: 24260555 and PMID: 18776146). Mouse experiments were approved by the University of Manchester ethics committee and the UK Home Office (licence PAFFCI44F). Mouse ureters were extracted from embryonic stage 15 mice and then cultured in serum-free defined media according to an established protocol (PMID: 24260555 and PMID: 18776146). There were 4 treatment conditions. Controls were grown in basal media or in the presence of 5 nanogram per milliliter recombinant human transforming growth factor-beta (TGFB1) for 1 day or 6 days. Each n=1 in the RNA-sequencing analyses comprised RNA extracted from the a set of six to eight ureters from the same litter grown in one condition for one or six days. Comparisons (n=3 for each experimental set) were made with paired ureter sets derived from the same embryonic litters. Mouse experiments were approved by the University of Manchester ethics committee and the UK Home Office (licence PAFFCI44F). Organs were collected in RNA protect (Thermo Fisher Scientific, Runcorn, UK) and RNA extracted using the RNeasy Plus Mini Kit (Qiagen).  Truseq stranded mRNA Assay
UNIVERSITY OF MANCHESTER
E-MTAB-7395:Sample 9_p
Experimental Factor: stimulus
none
Experimental Factor: time
1