GSM4368714
GSM4368714: Dam_CD_4days 3; Drosophila melanogaster; OTHER
Three biological replicates were used for each condition, and each replicate consisted of 300 heads. For tissue collections, briefly heads were collected using pre-chilled sieves in liquid nitrogen on dry ice. Frozen heads were then lysed. DNA was extracted from frozen heads following kit instructions. For identification of methylated regions briefly, purified DNA was digested by DpnI followed by PCR purification of digested sequences. TaDa adaptors were ligated by T4 DNA ligase . Adapter ligated DNA was PCR amplified according to protocol, and subsequently purified. Purified DNA was digested with DpnII followed by sonication to yield fragments averaging 200-300bp. TaDa adaptors were removed from sonicated DNA by digestion. Libraries were generated using the Takara ThruPlex kit (cat #022818) using 3ng input and 10 cycles of PCR. All libraries were sequenced on the Illumina NextSeq platform (High-output kit v2 75 cycles).
GEO
GSM4368714
GEO Accession
GSM4368714