GSM4368464
GSM4368464: TC71 scRNA-Seq; Homo sapiens; RNA-Seq
When the cells were around 70-80% confluent, the cells were washed twice with HBSS (Corning), recollected using 0.25% Trypsin, 2.21mM EDTA, 1X sodium bicarbonate solution (Corning) and centrifuged for 5 minutes at 300 rcf at 4 °C. Cell pellets were then resuspended in cold DPBS (containing no calcium and no magnesium) (Corning) supplemented with 0.04% Bovine Serum Albumin (BSA) to minimize cell sticking. Viable cells were consequently counted using an automated cell counter (Nexcelom), centrifuged as above described and resuspended at the desired final concentration in cold DPBS (containing no calcium and no magnesium) - 0.04% BSA. Finally, single cell suspensions were strain through a 70 um cell sieve and processed for sequencing. Immediatly prior to processing on the 10X single cell controller, cell density and viability were checked one last time by BioRad TC20 Automated Cell Counter by mixing 10ul cells and 10ul 0.4% trypan blue stian and also reviewed under microscope 10X Genomics Chromium Single Cell 3' V3.1 kit
GEO
GSM4368464
GEO Accession
GSM4368464