GSM4368931
GSM4368931: Mr lc-2575; Metarhizium robertsii ARSEF 2575; Bisulfite-Seq
CTAB DNA extraction The genomic DNA was randomly interrupted to 200-400 bp using Covaris M220; End-repairing the DNA fragment after interrupted, adding A tail, and ligating the sequencing linker in which all cytosines are methylated; followed by Bisulfite treatment (using EZ DNA Methylation Gold Kit, Zymo Research), after treatment, C which has not undergone methylation becomes U (changes to T after PCR amplification), and methylated C remains unchanged, and finally PCR amplification is performed to obtain a final WGBS library
GEO
GSM4368931
GEO Accession
GSM4368931