GSM2175743
GSM2175743: M81 5 min Harr treated 01; Homo sapiens; OTHER
Lysates were clarified by centrifugation and ribosome covered RNA was isolated by sucrose gradient ultracentrifugation and subsequent fractionation. Isolated fractions were RNAse I treated for 15 minutes at room temperature. Following nuclease treatment ribosome protected fragments were gel purified. Libraries for highthroughput sequencing were generated according to the published protocol from Ingolia et al. 2012 published in Nature Protocols.
GEO
GSM2175743
GEO Accession
GSM2175743