GSM2175162
GSM2175162: PMM rep2 [MeDIP-seq]; Sus scrofa; MeDIP-Seq
Genomic DNA extracted using the DNeasy Blood & Tissue Kit (Qiagen) according as the manufacturer’s instructions. Genomic DNA sonicated to approximately 100-500 bp fragments with a Bioruptor sonicator (Diagenode, NJ, USA). Then, libraries were constructed using a Paired-End DNA Sample Prep kit (Illumina, CA, USA) according as the manufacturer’s instructions. The fragments were ligated with adaptor and immunoprecipitated by monoclonal anti-methylcytidine antibody (Diagenode, NJ, USA). The enriched fragments with methylation and 10% input DNA were purified (DNA Clean & Concentrator-5 columns) and amplified by adaptor-mediated PCR. Then the MeDIP libraries were subjected to paired-end sequencing using Illumina HiSeq 2000 and a 50 bp read length.
GEO
GSM2175162
GEO Accession
GSM2175162