GSM2695688
GSM2695688: R4_7dpi; Danio rerio; RNA-Seq
Hearts were dissected, placed in sterile PBS to remove the atrium or bulbus arteriosus and the remaining ventricle was subsequently flash frozen in liquid nitrogen and stored at -80ºC. Total RNA from three or four ventricles was isolated and homogenized in 150 µl of QIAzolLysis Reagent. Pooled samples were processed with the miRNeasy MicroKit (Qiagen). The miRNeasy Micro Kit DNase treatment was included for RNA-seq analysis to remove genomic DNA, following the manufacturer's specifications. RNA libraries were prepared for sequencing using standard Illumina protocols (NebNext Ultra) RNA-Seq (poly-A capture)
GEO
GSM2695688
GEO Accession
GSM2695688