GSM4200327
GSM4200327: FOS4; Stenotrophomonas maltophilia; RNA-Seq
Twenty ml of each bacterial culture were spun down at                     6,000 xg for 3 min at 4 ºC and immediately frozen on dry ice and stored at -80                     ºC. RNA was isolated from cell pellets using the RNAeasy minikit (QIAGEN)                     following manufacturer's instructions. To remove remaining genomic DNA, total                     RNA samples were treated with DNAase I [RNase-Free DNAse Set (QIAGEN)], and a                     second digestion was then performed following the Turbo DNA-free (AMBION)                     protocol. The RNA was purified and concentrated using RNAeasy minikit columns.                     Only RNAs containing no DNA contamination were used for further studies. To                     analyze the transcriptome of S. maltophilia D457 and mutant strains, RNA was                     obtained from three independent cultures of each strain, which were then pooled                     to reduce biological variability. RNA libraries were prepared using 5 µg of RNA,                     rRNA was depleted using RiboZero kit and cDNA was                     synthesized.
GEO
GSM4200327