GSM2935652
GSM2935652: 521 small RNA-Seq; Homo sapiens; ncRNA-Seq
Total RNA was obtained using TriPure Reagent (Roche Applied Science, Indianapolis, IN, USA), following manufacturer's instructions. Total RNA samples were used and sample RNA Integrity Numbers were in the range 7.3 to 8.45 (average 7.8) when assayed on an Agilent 2100 Bioanalyzer. Sample amounts were 1µg of total RNA. They were processed with kit "TruSeq Small RNA Sample Prep Kit" (Illumina part # RS-930-1012) by following manufacturer instructions (Truseq SmallRNA SamplePrep Guide 15004197-E). Briefly, samples were sequentially ligated to 3' and 5' adapters, with the truncated form of T4 RNA Ligase 2 and the T4 RNA ligase 1, respectively. Reverse transcription with SuperScript II Reverse Transcriptase followed, yielding cDNA adapter-ligated and indexed libraries that were amplified by PCR with Illumina RNA PCR primers (13 cycles). Amplified barcoded cDNA from different samples was pooled, and a polyacrylamide gel electrophoresis fraction of 145 to 150 bases was extracted. The purified fraction was applied to an Illumina flow cell for cluster generation, and sequenced on an Illumina Genome Analyzer IIx (GAIIx), by following manufacturer's protocols. Sequencing read ends are expected to contain a variable length of the 3' adaptor, whose sequence is TGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC.
GEO
GSM2935652
GEO Accession
GSM2935652