ZXGN-2016
Transcriptome sequencing of Hynobius chinensis: adult male liver
Total RNA was immediately extracted (Omega Bio-Tek, USA) from tissue sample with TRIzol Regent (Invitrogen, CA, USA). The purity, concentration and integrity of RNA were assessed by Nanodrop, Qubit 2.0 and Agilent 2100 bioanalyzer respectively. And, using 1g of total RNA as input, a non-directional library for Illumina sequencing was prepared following the NEBNext Ultra RNA protocol. And, the library preparation was sequenced on an Illumina HiSeq 2500 platform (paired-end reads, 125 bp).
ZXGN-2016
SUB4145557
ZXGN-2016