Fecal877
Culture vs. Amplicon sequencing: a large-scale comparative study between 3,538 samples from healthy children
DNA was extracted from 200 l sample, using the PowerMag Soil DNA Isolation Kit optimized for epMotion and the epMotion robotic platform model. Two-step PCR of the v4 region was performed, First 30 cycles with broad range primers (515F and 806R), then 15 cycles to add sequencing primers and adaptors. 250 bp Paired-end sequencing, of up to 192 samples, were performed on the Illumina MiSeq System using MiSeq Reagent Kits v2
Fecal877
SUB5591902
Fecal877