GSM2935820
GSM2935820: MRA5 mRNA-seq; Homo sapiens; RNA-Seq
Total RNA was isolated using miRNeasy kit from Qiagen. RNA quality was verified using a 1% TBE gel as well as a Bioanalyzer. RNA concentration was estimated using the Bioanalyzer. Total RNA was processed as per manufacturer's instructions using the Illumina TruSeq Stranded mRNA kit for RNA-seq and TruSeq Small Library kit for miRNA-seq and sequenced using Illumina's HiSeq 2500 platform. Raw reads were analyzed for quality using FASTQC and mapped to known transcripts in human genome version hg19 using Salmon. The Salmon index for mapping was built using a k value of 25 and the UCSC genome browser CDS data hg19.RNA libraries were prepared for sequencing using standard Illumina protocols
GEO
GSM2935820
GEO Accession
GSM2935820