GSM1821765
GSM1821765: bas1-2; Saccharomyces cerevisiae; RNA-Seq
15 OD units of cells were collected for each sample from meiosis 4 hr. Cells were resuspended in 500 μl Complete Buffer A (50 mM NaAc pH 5.2, 10 mM EDTA, 1% SDS). 750 μl equilibrated RNA phenol was added. Cells were disrupted by incubating at 65°C for 5 minutes with vortex for 10 seconds every minute and a half and placing on ice for 3 minutes. After spinning in a mini centrifuge at 13,000 rpm for 6 min, the bottom layer was removed. Phenol incubation and vortex were repeated, but the top layer was transfer to a new 1.5 ml tube this time. RNA was purified by two rounds of phenol/chloroform extraction, one round of chloroform extraction and ethanol precipitation. After ethanol precipitation, the pellets were resuspended in 100 μl TE (10 mM Tris-HCl pH 6.8, 1 mM EDTA). The RNA-seq library was prepared following Illumina standard protocol. We used Trueseq RNA sample prep kit.
GEO
GSM1821765
GEO Accession
GSM1821765