GSM1438662
GSM1438662: Mock Day7; Aedes aegypti; ncRNA-Seq
Total RNA was extracted from non-infected control and DENV-infected mosquitoes (1, 3, and 7 d after infection) by using Tri-Reagent following the manufacturer’s protocol (Molecular Research Center). RNA concentrations were measured by using a spectrophotometer, and integrity was ensured through analysis of RNA on a 1% (wt/vol) agarose gel and bioanalyzer. Small RNA libraries were generated from both samples by using the Illumina Truseq Small RNA Preparation kit at LC Sciences. The purified cDNA libraries were sequenced on Illumina GAIIx, and raw sequence reads (36 nt) were obtained by using Illumina’s Sequencing Control Studio software version 2.8 followed by real-time sequencing image analysis and base-calling by Illumina’s Real-Time Analysis version 1.8.70 (LC Sciences).
GEO
GSM1438662
GEO Accession
GSM1438662