P285_50
Candona sp. microbiome
Pro_V34 bacterial 16 rRNA gene fragments were amplified using universal primers.Phusion Hot Start II High-Fidelity DNA polymerase (Thermo Scientific #F-549S) with High-FidelityBuffer was used for the amplification.For this purpose, concentration of the PCR products was controlled via capillary electrophoresis on a Shimadzu Multi-NAinstrument (DNA-12000 reagent kit). Libraries for Illumina MiSeq analysis were prepared with NEBNextUltra II DNA Library Prep Kit (New England Biolabs). The libraries were analysed using the IlluminaMiSeq Standard Kit v. 2,5 (Illumina) at the Evrogen Joint Stock Company (Moscow)
P285_50
SUB6480529
P285_50