GSM3712532
GSM3712532: 14513X1 KO input; Mus musculus; ChIP-Seq
Lysates were clarified from sonicated nuclei and TCF7L2-DNA complexes were isolated with antibody. Libraries were prepared according to DNA SMART ChIP-Seq Kit (Takara #634865)  Briefly,  a priming site is first added to the 3' end of the DNA template using the Terminal Deoxynucleotidyl Transferase. This is followed by annealing of a proprietary DNA SMART Poly(dA) Primer, which anneals to the T-tail added by the Terminal Deoxynucleotidyl Transferase. This primer is then used by the SMARTScribeTM Reverse Transcriptase (RT) to copy the DNA strand. When the SMARTScribe RT reaches the 5' end of the DNA template, the enzyme's terminal transferase activity adds a few additional nucleotides to the 3' end of the newly synthesized DNA. The carefully designed DNA SMART Oligonucleotide base-pairs with these additional non-template nucleotides and creates an extended template, enabling the SMARTScribe RT to continue replicating to the end of the oligonucleotide. Sequencing libraries are then generated by PCR-mediated addition of Illumina adapters using primers compatible with regions on the DNA SMART Poly(dA) Primer and the DNA SMART Oligonucleotide
GEO
GSM3712532
GEO Accession
GSM3712532