M2729
RNAseq of Paramecium bursaria Dd1:Green cell
Around 1E+05 of symbiotic Dd1 in the early stationary phase were collected by water pipe equipped with 11m pore size nylon membrane (NY1102500, Millipore). The cells were washed with modified Dryls solution twice. The total RNAs were extracted by TRI Reagent (T9424, Sigma-Aldrich) and RNeasy Mini kit (Cat No. 74106, QIAGEN) following instructions provided by the manufacturer. The library is prepared with Illumina TruSeq Stranded mRNA LT Sample Prep Kit by Genomics core facility (IMB, Academia Sinica, Taiwan) and sequenced by Illumina Nextseq platform.
M2729
SUB6042154
M2729