GSM4143934
GSM4143934: Mouse healthy colonic ICC: Lepob/+;KitcopGFP/+; Mus musculus; miRNA-Seq
Whole colonic or jejunal GI tissue was extracted and dispersed for cellular dissociation and prepared for cell sorting. Utilizing Fluoresence Activated Cell Sorting (FACS), cells positive for GFP signal were isolated as these cells represent Kit+ in GI tissue, which are ICC. These isolated KitcopGFP/+ cells were then placed into Lysis/Binding Buffer from the mirVana miRNA isolation kit (Thermo-Fisher) and extracted under manufacturer protocol. Samples from same genotypes (15 each) were pooled together to create the sample ready for processing and sequencing. miRNA libraries were prepared for sequencing using standard Illumina protocols
GEO
GSM4143934
GEO Accession
GSM4143934