GSM2936700
GSM2936700: Input of ChIpseq for H3K9me3 in wt mES cells, replicate 3; Mus musculus; ChIP-Seq
10^7 mES cells were crosslinked, chromatin was extracted and fragmented with micrococcal nuclease to mostly mono nuclesomes. 1% of chromatin was set aside as Input control. The chromatin was incubated with antibody over night, Protein A coupled Dynabeads were added for 2 hours and thereafter immunoprecipitated DAN was washed and de-crosslinked. Purified DNA was subsequently used for library preparation. For a detailed protocol, see Ostapcuk et al, 2017. Libraries were prepared using the NEB Next DNA library prep kit according to manufacturers instructions. For barcodes, see sample characteristics.
GEO
GSM2936700
GEO Accession
GSM2936700