GSM4512394
GSM4512394: p1035898-1 [ngs-78k68xt4d9_S7]; Homo sapiens; RNA-Seq
Peripheral blood mononuclear cells (PBMCs) and neutrophils (PMNs) were collected using LSM (Gibco) and Polymorphprep™ (Alere Technologies, Oslo, Norway). Fresh PBMCs (target 4,000/patient) and PMNs (target 1,000/patient) were combined and loaded at a concentration of 1000 cells/µL into a 10x Genomics Single Cell A v2 chip into the Chromium controller. Following single cell emulsion generation, uniquely identifiable first strand template cDNA was generated by emulsion PCR and after emulsion was broken, the second cDNA strand was generated and Illumina compatible adapters were ligated.
GEO
GSM4512394
GEO Accession
GSM4512394