Casp7_Lib3_sorted
DMS of human Caspase-7, Library 3, sorted
A CASP gene library was grown in a liquid culture and active enzyme variants were isolated via microfluidic screening. The plasmids for the active CASP variants were extracted, the CASP gene insert was digested out of the expression vector, and isolated via gel extraction. The CASP gene insert was then randomly fragmented using the Nextera XT kit and sequenced using a 2x300 MiSeq v3 kit.
Casp7_Lib3_sorted
SUB7240728
Casp7_Lib3_sorted