GSM2938761
GSM2938761: H460-Sensitive; Homo sapiens; Bisulfite-Seq
DNA was extracted using conventional techniques of digestion with proteinase K followed by phenol/chloroform extraction. Genomic DNA (2 μg) was spiked with unmethylated λ DNA (Promega) at a concentration of 5 ng of λ DNA per 1 μg of genomic DNA. The short-insert paired-end library was prepared using the TruSeq™DNA Sample Preparation Kit v2 (Illumina Inc.) following manufacturer's guidelines with minor modifications. In brief: The DNA was sheared with a Covaris E220 (Covaris) to 50–500 bp and size-selected to 150-300 bp fragments using AMPure XP beads (Agencourt Bioscience Corp.). DNA fragments were end-repaired, adenylated and ligated to Illumina specific indexed paired-end adaptors. After adaptor ligation, the DNA was treated with sodium bisulfite using the EpiTect Bisulfite kit (Qiagen) following the manufacturer's instructions for formalin-fixed and paraffin-embedded (FFPE) tissue samples with two rounds of conversions. After bisulfite conversion the adaptor-ligated DNA was amplified with 7 cycles of PCR using the PfuTurboCx Hotstart DNA polymerase (Stratagene). The library was quality controlled using BioAnalyzer 7500 assay (Agilent).
GEO
GSM2938761
GEO Accession
GSM2938761