17128C
whole genome amplified miracidium
Miracidia were place on Whatman FTA indicating cards for long-term storage. Individual miracidia were extracted from Whatman cards using a Whatman Harris 2mm micro-core punch and washed with 3x with FTA buffer and 2x with TE buffer. Whole genome amplification was performed using Illustra Ready-To-Go GenomiPhi V3 DNA Amplification kits. 200ng of WGA DNA was used to construct libraries using the KAPA HyperPlus kit. Samples were given unique indexes using IDT for Illumina TruSeq UD Indexes, multiplexed into a pooled library, and sequenced on a single Illumina NovaSeq lane using 150bp paired-end sequencing.
17128C
SUB7849750
17128C