Pat7-27_S5500
SOLID5500xl data of Pat7-27
20 ng of total RNA were amplified using the Ovation RNA-Seq System V2 (Nugen), resulting in a final concentration of 2.5 µg double stranded SPIA cDNA. Following purification of cDNA using the MinElute Reaction Cleanup Kit (Qiagen), the cDNA was fragmented by ultrasonic shearing to the appropriate size (150-300 bp) using the Covaris S2 System. 1 µg of fragmented cDNA was subsequently used for the automated library construction on a Beckman Coulter SPRIworks platform.
Pat7-27
Universitaetsklinikum Erlangen
Pat7-27_S5500