GSM3502730
GSM3502730: Tick_saliva_1A; Haemaphysalis longicornis; miRNA-Seq
The collected saliva was put in trizol and total RNA was extracted according to the manufacturer's protocol. Use Agilent 2100 Bioanalyzer（Agilent RNA 6000 Nano Kit）to test sample integrity and concentration, and NanoDrop to Inorganic ions or polycarbonate contamination. Small RNA library was constructed according to the TruSeq Small RNA Sample Pre Kit (Illumina). Use the 200ng-1μg of RNA sample, then separate RNA segment of different size by PAGE gel,select 18-30nt（14-30 ssRNA Ladder Marker，TAKARA) stripe and recycle；
GEO
GSM3502730
GEO Accession
GSM3502730