GSM4200659
GSM4200659: 160WTNAc; Mus musculus; RNA-Seq
Brains were rapidly extracted and frozen in a dry ice bath containing isopentame. 300 micron thick sections containing the basolateral amygdala (BLA) and Nucleus Accumbens (NAc) was cut on the cryostat and brain regions were punched using a micropunch. RNA was extracted using the Magmax-96 kit from Ambion. DNA contamination was removed by DNAse treatment. RNAseq libraries were prepared for sequencing using standard illumina protocols using the NextSeq 500/550 High Output kit, Illumina Inc., San Diego, CA polyA enriched RNAs (MicroPolyA purification kit, Life Technologies, Carlsbad, CA) were used for library construction
GEO
GSM4200659
GEO Accession
GSM4200659