Eyes of S.tileihornes
RNA-Seq of Sinocyclocheilus tileihornes : adult eyes
Total RNA was isolated using a TRIzol reagent (Invitrogen) from whole eye of adult cave species Sinocyclocheilus tileihornes. Two individuals were used. Oligo(dT) selection was performed by using MicroPoly(A) PuristTM (Ambion) according to the manufacturer's protocol. Superscript III First-Strand Synthesis System (Invitrogen) was used to create first-strand cDNA. The second-strand cDNA was synthesized using random primers and Klenow exo- polymerase (Epicentre). cDNA was sheared to short fragments with Biorupter Sonicator, and then the fragments were connected with sequencing adapters. After size selection, suitable fragments were used as templates for PCR amplification. Finally, four libraries (200  400 bp insert) were sequenced using Illumina sequencer HiSeq 2000 paired-ed 100-nt (Illumina Inc., San Diego, CA, USA).
Eyes of S.tileihornes
SUB4100493
Eyes of S.tileihornes