JAXcoCR.D22.rep4
16S amplicon sequencing of feces from C57BL/6J mice cohoused with C57BL/6NCrl mice for 22 days that became resistant to Salmonella infection
Primers 515F and 806R were used to amplify the V4 domain of the 16S rRNA. Each sample was barcoded with a unique forward and reverse barcode combination. PCR contained 1 Unit Kapa2G Robust Hot Start Polymerase, 1.5mM MgCl2, 10 mol of each primer, 10mM dNTP s, and 1ul of DNA. PCR conditions were: an initial incubation at 95 C for 2 min, followed by 30 cycles of 95 C for 20 s, 50 C for 20 s, 72 C for 20 s and a final extension of 72 C for 3 min. The final product was quantified on the Qubit instrument using the Qubit High Sensitivity DNA kit and individual amplicon libraries were pooled.
JAXcoCR.D22.rep4
SUB4405115
JAXcoCR.D22.rep4