GSM3639269
GSM3639269: OE-Dppa2_rep1; Mus musculus; RNA-Seq
For RNA-seq, cells were treated with TRIzol and followed with phase seperation, RNA precipitation and RNA wash. Finally, RNA were dissolved in DEPC water. For RNA-seq, total RNA was subjected to two rounds of purification using poly-T oligo-attached magnetic beads before the synthesis of double-stranded (ds) cDNA. The ds cDNA was ligated to adaptors and sequenced with illumina Hiseq2000 platform.
GEO
GSM3639269
GEO Accession
GSM3639269