N1
454 16S rRNA amplicon sequencing
A universal primer set targeting the hypervariable V6-V8 16SrRNA gene region was used to amplify sample DNA using PCR. PCR products were purified using GeneJet Purification Kit (Fermentas) and unidirectionally sequenced using 454 Roche GS FLX titanium technology.
N1
SUB4142136
N1