GSM2945818
GSM2945818: sc-RNA-seq_JNKi DE single cell_1; Homo sapiens; RNA-Seq
Definitive endoderm cells were washed with PBS once and treated with TrypLE for 5 minutes. E8 media was added to stop the digestion. Dissociated single cells were resuspended in PBS with 0.01% BSA, passed through a 40 micron filter and counted.  Cell concentration was adjusted to 100-120 cells/μl with >90% viability (in 1 ml volume). Cell suspension were kept on and transferred to the Weill Cornell Genomics and Epigenomics Core Facility for drop-seq. Drop-seq experiment procedure follows the protocol published by the Steve McCarroll's lab version 3.1 (12/18/15) http://mccarrolllab.com/download/905/.
GEO
GSM2945818
GEO Accession
GSM2945818