skin tissue of A. davidianus
skin tissue of A. davidianus sequencing
After RNA quality control, small RNAs of 16-30 nt in length were isolated from total RNA by size fractionation with 15% TBE urea polyacrylamide gel and then adaptors were ligated to the 5′ and 3′ terminals of these small RNAs. Afterwards, reverse transcription followed by RT-PCR was used to create cDNA constructs, which were then purified on 6% Novex TBE PAGE gel and sequenced at the Beijing Biomarker Technologies Co., Ltd. using Illumina HiSeq 2500 platform. Sequences were extracted from image files using the Illumina pipeline.
College of Animal Science and Technology, Henan University of Science and Technology
Henan University of Science and Technology
skin tissue of A. davidianus