GSM4514127
GSM4514127: 007-9(WT-L.m infection 5h-3); Mus musculus; RNA-Seq
The cells were stored in liquid nitrogen immediately after mature and then transferred to the -80℃ freezer. Total RNA was extracted from the frozen cells using the TIANamp DNA/RNA Isolation Kit (TIANGEN), including additional treatment with RNase-free DNase I (Ambion) for 30 minutes at 37℃ to remove contaminating DNA.18-30nt RNA segments were separated and recovered by PAGE gel. The steps which followed were the same as for generating the mRNA sequencing library from the addition of poly-A. The miRNA DNBs were loaded into the patterned nanoarrays and single-end reads of 50 bp were read through on the BGISEQ-500 platform at Beijing Genomics Institute (BGI; Shenzhen, China). RNA libraries were prepared for sequencing using standard Illumina protocols
GEO
GSM4514127
GEO Accession
GSM4514127