Casp7_Lib1_unsorted
DMS of human Caspase-7, Library 1,  unsorted
A CASP gene library was generated via error-prone PCR, cloned into an expression vector, and transformed into C43(DE3) cells. This transformed library was then grown in a liquid culture, the plasmid DNA was extracted, the CASP gene insert was digested out of the expression vector, and isolated via gel extraction. The CASP gene insert was then randomly fragmented using the Nextera XT kit and sequenced using a 2x300 MiSeq v3 kit.
Casp7_Lib1_unsorted
SUB7240728
Casp7_Lib1_unsorted