qiita_ptid_3952:11402.MC082
Illumina MiSeq sequencing; qiita_ptid_3952:11402.MC082
A cross-sectional observational study of the gut microbiome in Peruvian infants at 6 or 12mo of age
11402.MC082
Sequences were amplifed using the F515/R806 primer pair, both of which were modified to contain an Illumina adapter region. An 8bp Hamming error-correcting barcode included on the 3' end of the forward primer to allow for sample multiplexing.
UCSDMI
qiita_ptid_3952:11402.MC082
University of California San Diego Microbiome Initiative
qiita_ptid_3952:11402.MC082
barcode
ACTGAGAG
center_name
UC Davis Slupsky Lab
center_project_name
Peruvian_Infant_Iron_Microbiome
linker
GT
linker_primer_sequence
GTGTGCCAGCMGCCGCGGTAA
pcr_cond
94degC_3min; denaturation:94degC_45sec; annealing:50degC_1min; elongation: 72degC_90sec; final elongation:72degC_10min; 40total cycles
pcr_primers
FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
primer
GTGCCAGCMGCCGCGGTAA
reverse_primer
GGACTACHVGGGTWTCTAAT
run_center
UC Davis Genome Center
run_date
3/3/2016
samp_vol_we_dna_ext
225.4
sequencing_meth
Sequencing by synthesis
target_gene
16S rRNA
target_subfragment
V4