GSM4292954
GSM4292954: LYS14_ALL_Kang2020; Saccharomyces cerevisiae; OTHER
Genomic DNA was extracted from all cells using standard glass bead-beating, phenol chloroform isoamyl alcohol purification, and NH4Ac ethanol protocol. Genomc DNA was digested in three separate restriction enzyme digests (HinPI1, HpaII, and Taq-alphaI), self-ligated with T4 DNA ligase, and an inverse PCR reaction was performed to amplify genomic DNA downstream of each inserted Ty5 retrotransposon. "Calling Card" Assay to identify in vivo genomic targets of DNA-binding factors
GEO
GSM4292954
GEO Accession
GSM4292954