E-MTAB-6592:Sample 21_p
Illumina HiSeq 2500 paired end sequencing; RNA-sequencing of human embryonic and fetal urinary bladder and genital tissues
RNA-sequencing of human embryonic and fetal urinary bladder and genital tissues
Sample 21_p
Embryonic and fetal bladders and genital tissue were obtained by surgeons from terminated pregnancies after informed consent and with ethics approval. Samples were taken from weeks 7 (n=2), 7,5 (n=1), 8 (n=3), 9 (n=4) for the bladder tissues and from weeks 7 (n=3), 8 (n=3), 9 (n=3) as well as 10 (n=4) for the genital tissues, respectively.   Fresh tissues were dissected and immediately submerged in 5 volumes of RNAlater Stabilization Solution (Cat No. AM7020, Thermoisher Scientific, Waltham, MA), incubated at 4°C overnight and then transferred to -20°C. RNA was extracted by using TissueLyser LT (Cat No. 85600, QIAGEN, Hilden, Germany) and RNeasy Fibrous Tissue Mini Kit (cat. no. 74704, QIAGEN, Hilden, Germany). The RNA was measured both quantitatively and qualitatively using NanoDrop (Thermofisher Scientific, Waltham, MA) and Bioanalyzer 2100 (Agilent, Santa Clara, CA) instruments.   Paired-end libraries of 2x126 bp with poly-A selection according to Illumina TruSeq Stranded mRNA protocol.
Karolinska Institutet, Department of Women's and Children's Health, Center for Molecular Medicine
E-MTAB-6592:Sample 21_p
Experimental Factor: age
10
Experimental Factor: organism part
reproductive organ