GSM3876334
GSM3876334: MC57G cells, Xrn1 siRNA, mock infection Rep 1 [input DNA]; Mus musculus; ChIP-Seq
Lysates were clarified from sonicated nuclei and PolII-DNA complexes were isolated with antibody. Libraries were prepared using the KAPA Hyper prep Kit (KAPA KK8504). Briefly, 20ng of ChIP'd or input DNA for each sample was subjected to end repair and A-tailing. Illumina adapters were ligated and then the samples were PCR amplified with Illumina primers for 15 cycles. The purified DNA was sequenced on a Hi Seq 4000
GEO
GSM3876334
GEO Accession
GSM3876334