GSM3399757
GSM3399757: Sample_81791_FBTreg; Mus musculus; RNA-Seq
Cell lysates of FACS sorted cells were homogenized using QIAshredder, then total RNA samples were isolated using RNeasy Plus Micro Kit (Qiagen), including an on-column DNase treatment using RNase-Free DNase Set (Qiagen) according to manufacturer's instruction. RNA concentration and quality was determined and samples with RNA Integrity Numbers (RINs) of 8 or greater were prepped for strand mRNA libraries by the University of Michigan Sequencing Core. Libraries were multiplexed (6 per lane) and sequenced using paired end 50 cycle reads on a HiSeq 4000 (Illumina).
GEO
GSM3399757
GEO Accession
GSM3399757