GSM4455326: CVID 1 21pos; Homo sapiens; RNA-Seq
Total RNA was purified from sorted B cell subpopulations using the Qiagen Rneasy Microkit according to the manufacturer's instructions (Qiagen) Samples were processed using the NEBNext Directional Ultra RNA 2 (NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina, cat # E7760L) protocol from NEB, with 10 ng of input total RNA and with 15 PCR cycles. Samples were sequenced 84-bases long on 2 lanes of NextSeq® 550 with high output flowcell (NextSeq® 500/550 High Output Kit v2).
GEO Accession
GSM4455326