GSM2090082: PFL_E12_WT_RNAseq_rep1; Mus musculus; RNA-Seq
Micro-dissected proximal and distal segments of E12.5 limbs either from wild type or Hoxa13d13-dKO mutant embryos were used for RNA extraction. Samples were stored in RNAlater at -80°C. The distal or proximal portion of the forelimbs from individual embryos was processed using the RNAeasy micro kit (Qiagen) according to manufacturer instructions. On-column DNase I treatment was performed during 20min at room temperature to ensure pure, DNA-free RNA extraction. A total of 100 ng of pure total RNA was amplified following standard Illumina procedure for polyA-selected RNA. RNA sequencing (RNA-seq) libraries were prepared with the Illumina TruSeq Stranded mRNA protocol and sequenced on a HiSeq 2500 machine, as single-end, 100 base pairs (bp) reads. The preparation of libraries and sequencing were performed by the genomic platform of the University of Geneva.
GEO Accession
GSM2090082