RNA-Seq of Fall armyworm midgut with Cry1Ab diet replicate 02
For the unexposed control treatment, the strain susceptible to the population Cry1A.105 + Cry2Ab2, here called SUS, was created under laboratory conditions. In the second treatment, FAW larvae collected in the city of Lucas do Rio Verde in corn fields in the state of Mato Grosso in Brazil were used. The population resistant to the FAW's Cry1A.105 + Cry2Ab2 (next generation) in common characteristics of Bt maize, called Cry R, were raised on an artificial diet adapted from Greene et al., 1976 and kept under controlled conditions (temperature 25C  1C , humidity 70  10% and light: 14:10 h in the dark). When the larvae reached the stage of fourth-instar, the samples were separated for each treatment (resistant and non-resistant) and used for extraction of the midgut intestine. Then the midguts were sampled using liquid nitrogen and stored in -80  C for RNA extraction.