GSM4708464: Splenic EAE B cell; Mus musculus; RNA-Seq
Spleen was removed and placed in ice-cold RPMI 1640 medium, ground and filtered with 40 μm nylon mesh strainers.The splenic cells were incubated in RPMI-1640 (2×106cells/mL) with rMOG (10ng/mL) in a humidified incubator at 37°C with 5 % CO2 for 24h.After the B-cell sorting,the splenic cell suspension was generated to single-cell gel beads in the emulsion with the Chromium single-cell controller. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM4708464