TK421 raw PacBio reads
Libraries were prepared by shearing genomic DNA with g-TUBES targeting an average fragment size of 20 kb. SMRTbell template preparation kit v1.0 was used to generate libraries. Size selection was performed using BluePippin with 12kb cutoff. Sequencing was performed using P6-C4 chemistry and RS II SMRT Cells 8Pac v3 with 240-min movies on the RS II instrument.