GSM2088405: Sperm-HiC_replicate1_biological; Mus musculus; OTHER
For Hi-C, cells were crosslinked with 1% formaldehyde for 10 min at room temperature. Crosslinking was quenched with glycine and nuclear lysates were incubated with HindIII. Fragment ends were filled with biotinylated nucleotides, ligated. After reverssion of crosslinks, DNA was purified, sonicated and used to make Illumina libraries. Libraries for Illumina sequencing were constructed using the following standard protocol. The fragment end were repaired using the NEBNext End Repair Module, and adenosine was added at the 3’ ends of fragment using Klenow fragment (3’ to 5’ exo minus, New England Biolabs). Precipitated DNA and input DNA were incubated with adaptors at room temperature for 1 hr with T4 DNA ligase (New England Biolabs) and amplified with Illumina primers.
GEO Accession
GSM2088405