GSM3336638: EVC-pRK-1; Sinorhizobium meliloti; RNA-Seq
Total RNA  was extracted with 1 ml TRIzol (cell disruption was performed  in the cell suspension using glass beads in a shaking mill). Precipitated RNA was dissolved in cold ultrapure water and imediately subjected to additional hot-phenol purification. 10 µg RNA was treated with TURBO DNase and used for RNAseq.  RNA from three independent biological samples was pooled. Each pool was used for three independent cDNA libraries. The total RNA samples were first fragmented using ultrasound (4 pulses of 30 sec at 4°C). Each sample was used for three independent cDNA synthesis. First, an oligonucleotide adapter was ligated to the 3' end of the
GEO Accession
GSM3336638