GSM4454773: CITE-seq Aged_Ctrl v ABX mRNA/cDNA; Mus musculus; OTHER
Mice were perfused with PBS and brains extracted. Brains digested with papain-based multi-tissue dissocation kit (Miltenyi). Mononuclear cells enriched by 70%/37%/30% percoll gradient centrifugation. For RNA expression (cDNA), we followed 10x genomics single cell 3' v3 assay standard protocol. For ADT and HTO, we followed CITE-seq and Cell hashing protocol as provided here:https://citeseq.files.wordpress.com/2019/02/cite-seq_and_hashing_protocol_190213.pdf 10x genomics single cell 3' v3.1 assay
GEO Accession
GSM4454773