GSM3400984: hem1; Procambarus virginalis; ATAC-seq
Approximately 500 µL of hemolymph was collected from three independent animals. After washing the cell pellet twice with sterile and cold PBS 1X, 50.000 hemocytes were immediately used for the ATAC library preparation. The transposase reaction was optimized and a 20 min reaction was used to avoid DNA “over transposition”. The subsequent steps were as described in the original protocol (Buenrostro 2015). ATAC-Seq on Illumina HiSeq X platform.
GEO Accession
GSM3400984