GSM1162726: BSO19B_NuGEN; Homo sapiens; RNA-Seq
RNA was extracted from mid log phase cultures at low passage (P1 or P2) using Triazol kit (Invitrogen) For Illumina libraries, 5ug total RNA were processed according to the mRNA Seq Sample Preparation Kit protocol (part number 1004898 Rev A), with the 300bp gel fragment eluted and purified for sequencing. For NuGEN libraries, 500-800pg total RNA were amplified and converted to cDNA using NuGEN’s Ovation RNA-Seq kit. Following amplification, 4.6-5.0ug cDNA was fragmented to ~200bps using Covaris S2 and the fragmentation parameters described in the NuGEN  ENCORE NGS library preparation protocol. The remainder of the library preparation followed manufacturer’s protocol as described in NGS Library System I and Multiplex System, Part no. 300.
GEO Accession
GSM1162726