GSM2176455: Oocyst sporozoite, alba4-null Replicate 2; Plasmodium yoelii; RNA-Seq
Following purification of the parasite samples, RNA from all sample types was isolated by the QIAgen RNeasy Kit using the manufacturer’s protocol with the additional on-column DNaseI digestion. RNA yields were quantified spectrophotometrically, and the quality of all samples was confirmed by measuring RNA Integrity Number (RIN) using the Agilent Bioanalzyer. A barcoded library was made from each sample by using the Illumina TruSeq Stranded mRNA Library Prep Kit according to the manufacturer's protocol. qPCR was performed to determine the concentration of each library and an equimolar pool was made of all libraries.
GEO Accession
GSM2176455