rice1
Briefly, mRNA was isolated from total RNA using magnetic beads with Oligo (dT). Fragmentation was carried out using Fragment Mix. After poly (A) addition, the short fragments were connected with Illumina RNA adapter to prepare for hybridization. The suitable fragments (200 bp) were selected for the PCR amplification. After that, the library preparations were sequenced on an Illumina Hiseq 2500 platform.