GSM1444163: BSW007i5; Caenorhabditis elegans; RNA-Seq
RNA was extracted using a the protocol described in Baugh, Hunter Development 2002 except that 10 µL of a 20 mg/mL glycogen solution was used as a carrier. Libraries were prepared from 10 µg of total RNA. PolyA RNA was purified using the Dynabeads mRNA purification kit (Ambion) and fragmented using Fragmentation Reagent (Ambion). First strand cDNA was synthesized from polyA RNA using the SuperScript III Reverse Transcriptase Kit with random primers (Invitrogen). Second strand cDNA synthesis was performed using Second Strand Synthesis buffer, DNA Pol I, and RNAse H (Invitrogen). cDNA libraries were prepared for sequencing using the mRNA TruSeq protocol (Illumina).
GEO Accession
GSM1444163