CRISPRa secondary pool screened with Selumetinib using dCas9-VP64 in MelJuSo, Replicate C
MelJuSo cells expressing dCas9-VP64 were infected with the CRISPRa secondary pool (from ORF and CRISPRa screens), Selumetinib was added on Day 7, Day21 time point was collected, genomic DNA was PCR amplified and sequenced