GSM4089176: hrpk1_tm5522_L4_rep1; Caenorhabditis elegans; miRNA-Seq
RNA from embryos and L4 animals was prepared as previously described ( Zinovyeva et al 2014, PLOS Genetics https://doi.org/10.1371/journal.pgen.1004286) with the following modifications. Mixed stages animals resuspended in 250μL water were mixed with 1ml of Trizol (Fisher Scientific) and RNAse free Rhino beads (MIDSCI) and homogenized using a Bullet blender (MIDSCI) at the highest setting for 4 minutes. Homogenate was then moved into a fresh tube, mixed with 212μL of chloroform, and spun down to separate the phases. All remaining steps of the RNA purification were performed as previously described Small RNA libraries were prepared using the Bioo Scientific NEXTflex Small RNA-Seq Kit v3.
GEO Accession
GSM4089176