GSM2087089: two-weeks seedling plants_307T1_Bisulfite-seq; Oryza sativa; Bisulfite-Seq
For each sample, about 20 individual plants were pooled and total genomic DNA was extracted using CTAB method. DNA samples were treated with RNase A and DNA was purified from the supernatant using chloroform.  About 5 microgram of genomic DNA was sheared by sonication to fragments of 100-500 bp, and the adapters were ligated following the Illumina protocol. The library was then treated twice with sodium bisulfite (which converts unmethylated Cs to Us) using the Qiagen EpiTect kit, and amplified by 18 cycles of PCR using PfuTurboCx DNA polymerase (Stratagene), The PCR product was purified with AMpure XP beads to obtain the final library DNA. BS-seq libraries were sequenced using an Illumina HiSeq 2000. libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM2087089