V3-V4 16S-Seq of bacteria
Sample was taken from rainbow trout with clinical signs of Aeromonas infection. The sample was subjected to microbiological and molecular procedures for the isolation of Aeromonas. The DNA from collected microorganisms was isolated using the GeneMATRIX DNA Purification Kit (EurX). PCR amplification was carried out using Q5 Hot Start High-Fidelity 2X Master Mix with region-specific (341F and 785R) primers. Sequencing took place on an Illumina MiSeq sequencer using the MiSeq Reagent Kit.