GSM3263099: 06-Baloh-7438_S10; Mus musculus; RNA-Seq
The lumbar regions of the spinal cords were dissected away from the rest of the spinal cord and flash frozen on dry ice.  RNA was harvested using standard Trizol protocol.  Total RNA was isolated using the PureLink RNA Mini Kit (Catalog # 12183018A), and RNA integrity was determined using the Agilent 2100 Bioanalyzer.  2 ug of total RNA was used for the construction of sequencing libraries. RNA libraries were constructed and indexed in parallel using the Illumina TruSeq Stranded mRNA Library Prep Kit v2 (standard protocol).  Library integrities were determined using the Agilent 2100 Bioanalyzer, quantified using Qubit fluorometric quantitation, and pooled for sequencing.
GEO Accession
GSM3263099