GSM3401802: RNA pooled from 5 larvae fed on Asclepias curassavica replicate 1 [sAC-1]; Danaus plexippus; miRNA-Seq
Larvae were collected after 24 hours, frozen immediately in liquid nitrogen and stored at -80C until the total RNA extractions were performed. Triplicates of five whole larvae for each treatment were homogenized using a Tissue Lyzer. Total RNA was extracted using the Direct-zol kit (Zymo), using the same total RNA to prepare mRNA and sRNA libraries. The mRNA libraries were created using TruSeq RNA kit. The small RNA libraries were created using the Illumina TruSeq small RNA kit.
GEO Accession
GSM3401802