DNA amplicon survey peatland microbial communities
Sequenced at the U.S. Department of Energy Joint Genome Institute (JGI, Walnut Creek, California, USA; now in Berkeley, California) using the protocol of Caporaso et al. (2012), with small modifications (see Tremblay et al., 2015; doi: 10.3389 /fmicb.2015.00771., and  Coleman-Derr et al., 2016; doi: 10.1111/nph.13697). PCR amplification utilized the primers fITS9 (Ihrmark et al., 2012; DOI: 10.1111/j.1574-6941.2012.01437.x) and ITS4 (White et al., 1990; DOI:: 10.1016/b978-0-12-372180-8.50042-1.) targeting the fungal ITS2 region. Primers were fitted with Illumina sequencing adaptors and the reverse primer contained an 11bp barcode index. Sequencing used 2 x 300 bp chemistry.