GSM4202589: FO.Cr.24h.neg.2; Mus musculus; RNA-Seq
Spleens were harvested at time of saccrifice and lypmhocytes were stained and FACS sorted directly into TRIZOL reagent as follows:  follicular B cell: live, singlet, CD19+, CD93-, CD23+, CD21/35int.  marginal zone B cell: live, singlet, CD19+, CD93-, CD23-, CD21/35high.  In-vitro cultured were FACS sorted by live, singlet, CTV dilution and CD138 expression. RNA was extracted by TRIZOL protocol and quantified by Qubit.  cDNA was prepared using Takara Clonetech smarter v4 kit by protocol with Illumina Nextera XT reagents for library construction and quantified and equal molar pooled by tape station and qubit analysis.
GEO Accession
GSM4202589