GSM4453939: 2.Th1 cells differentiated from CD4+ T cells of WT mice for day 3.5; Mus musculus; RNA-Seq
Cells were directly collected in RLT-Lysis buffer (Qiagen) supplemented with beta-Mercaptoethanol according to the manual. Isolation of RNA was done using Qiagen's RNeasy plus mini kit. Quantity of total RNA was assessed with the Qubit 2.0 and quality was checked using a RNA 6000 nano chip on Agilent's bioanalyzer. Barcoded mRNA seq libraries were prepared from 700 ng total RNA using a NEBNext® Poly(A) mRNA Magnetic Isolation Module and NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® according to the manual. All quality controls were done using Invitrogen's Qubit HS assay and fragment size was determined on Agilent's 2100 Bioanalyzer HS DNA assay.
GEO Accession
GSM4453939