Whole genome DNA sequencing: fetal Micro36 isolate
The 24-h cultures of Micro36 were obtained in medium cetyltrimethylammonium bromide-based protocol. The gDNA was fragmented and Illumina adaptors were ligated using a Nextera XT (Illumina) kit following manufacturers instructions. The gDNA library quality was verified by a gel electrophoresis Bioanalyzer (Agilent) and was sequenced on an Illumina MiSeq using a MiSeq Reagent kit v.3 (Illumina) with 300 _ 300-bp paired-end reads. Reads were removed from adaptors and quality filtered using TrimGalore. When possible, paired-end reads were assembled using FLASh for use as a single- ended library for assembly using SPAdes genome assembler.