GSM4089211: 24D_Endurance_Post; Homo sapiens; RNA-Seq
RNA was isolated according to Life Technologies instructions accompanying the mirVana PARIS Kit (Part #AM1556) Libraries were prepared according to Life Technologies instructions accompanying the Ion AmpliSeq Transcriptome Human Gene Expression Kit (Part #A26325). Briefly, 50 ng of total RNA was reverse-transcribed to generate first strand cDNA using SuperScript™ VILO™ (Invitrogen) reverse transcriptase and random primers. Following reverse transcription, target genes were amplified using Ion AmpliSeq™ Transcriptome Human Gene Expression Core Panel. After target amplification, primer sequences were partially digested and amplicons were phosphorylated. The amplicons were then ligated to unique Ion Xpress™ barcodes (Life Technologies) and the resulting constructs were purified using Agencourt™ AMPure™ XP Magnetic Beads (Beckman Coulter). Libraries were quantified by qPCR against a standard curve of E. Coli DH10B, and normalized to 100 pM. Six libraries were pooled equally and 25 μL of the pooled mixture was used on one Ion 540™ Chip (Life Technologies) for template preparation using the Ion Chef™ Instrument (Life Technologies). Ion semiconductor sequencing was performed on the Ion S5 XL system (Life Technologies).
GEO Accession
GSM4089211