Illumina HiSeq 2000 sequencing; Marine diatoms sense and respond to microscale turbulence in non-limiting nutrient conditions
Marine diatoms sense and respond to microscale turbulence in non-limiting nutrient conditions
Diatoms were subject to a turbulent condition using a system of cyllinders called Turbogen. Two different experiments were carried out. One lasted after three days and the second after four. RNA was extracted using TriPure (Sigma) reagent following manufacturer’s instructions, gDNA was removed by DNase treatment (Sigma, DNase I recombinant, RNase-free). RNA was then cleaned-up by Qiagen RNeasy Mini Kit. The quality, integrity and quantity were estimated using an Agilent 2100 Bioanalyzer platform (Agilent Technologies). Libraries were prepared using a Beckman Biomek FX laboratory automation workstation and the Illumina® TruSeq® Stranded Total RNA Sample Preparation kit, following the standard procedure for poly A selection and starting with 500 ng total RNA.
Experimental Factor: 3 days water acceleration
stimulus