GSM3401636: mOSN_RNAseq_rep5; Mus musculus; RNA-Seq
Crosslinked-ChIP: Chromatin from formaldehyde cross-linked cells was sheared on a Covaris S220 Focused-ultrasonicator to a size range of 200-500bp. Approximately 1-2ug of sheared chromatin was used for immunoprecipitation with antibodies, and precipitated DNA was column purified. RNA: Cells were lysed in Trizol-LS (ThermoFisher) then RNA was extracted and treated with Turbo-DNase (ThermoFisher). Crosslinked-ChIP: ChIP-seq Libraries were prepared using a Nugen Ultralow V2 kit. RNA: RNA-seq libraries were prepared using a TruSeq Stranded Total RNA with Ribo-Zero Gold Set B kit (Illumina RS-122­2302).
GEO Accession
GSM3401636