Miseq of paired guide screen: dCas9KRAB
60 ug of genomic DNA divided over 60 reactions were amplified using KAPA2G Robust PCR kit (KAPA Biosystems). The products of all first-round PCR reactions from the same sample were pooled. 1 ul of the pooled product was used for a sample indexing in preparation for next generation sequencing using Illumina Truseq indexing primers.