GSM3039928: wild-type_1; Caenorhabditis elegans; ncRNA-Seq
RNA was extracted using Trizol (Ambion)  followed by isopropanol precipitation. RNA samples were treated with 20U RNA 5' polyphosphatase (Lucigen, RP8092H) for 30 minutes at 37°C to remove 5' triphosphate groups. Library preparation and sequencing was performed at the UNC School of Medicine High-Throughput Sequencing Facility (HTSF). RNA-seq libraries were prepared using the NEXTflex Small RNA Library Prep Kit for Illumina (Bioo Scientific). Four nucleotide barcodes were added to the 5' and 3' ends of inserts during library preparation. Libraries were sequenced on an Illumina HiSeq 2500 to produce approximately twenty million 50 bp single-end reads per library.
GEO Accession
GSM3039928