16S rDNA-Seq: grassland soil: Sample 1N15
"The total community DNA was extracted from soils by use of a MoBio Power Soil DNA extraction kit. The V1-V3 region of the 16S rRNA gene was amplified in triplicate using the barcoded 27F and 533R primer set. PCR products were quantified, purified and sequenced by Illumina Miseq platform for assessment of bacterial diversity and community structure. "