Acetic acid preculture after subsequent acetonitrile enrichment (replicate #1)
A soil community enriched in M9 medium containing 25 mM acetic acid and ammonium as sole carbon and nitrogen source was used as inoculum for baffled flasks containing 50 ml M9 medium containing 25 mM acetonitrile as sole carbon and nitrogen source. Incubation was done at 25 C and 180 rpm. Cells were harvested at an OD600 of approx. 1.0 by centrifugation at 10000 g for 1 min. RNA isolation was done with the Rneasy Mini Kit (Qiagen).