GSM3262275: 4C-seq E11.5 forelimb bud Twist1 4C-seq; Mus musculus; OTHER
Limb bud and branchial arch tissues of mouse E11.5 embryos were micro-dissected and dissociated into single cells that were fixed with 2% formaldehyde. Nuclear DNA was digested using DpnII and BfaI (4-bp cutters) as primary and secondary restriction enzymes, respectively, followed by ligation using T4 DNA ligase. Ligated fragments were amplified by inverse PCR for each viewpoint. PCR products were sequenced with a 50-bp single-end read on Illumina HiSeq machine.
GEO Accession
GSM3262275