RNA-seq of Ficus carica: control leaf after 24 days
Total RNA was isolated from leaves using Logemann et al 1987 protocol, followed by a DNAse I (Roche) digestion according to the manufacturers instructions, to remove genomic DNA contamination. Hence, RNA was purified by phenol/chloroform extraction and precipitated following standard procedures.RNA-Seq libraries were generated using the TruSeq RNASeq Sample Prep kit, according to the manufacturers protocol (Illumina Inc., San Diego, CA, USA). Then Poly-A RNAs were isolated from the total RNA and chemically fragmented. Libraries were run on the Illumina HiSeq2000 (Illumina Inc.) using version 3 reagents.