GSM4455358: LCL1-HDAC2 rep2; Homo sapiens; ChIP-Seq
30 million LCL1 cells were crosslinked with 1% formaldehyde and harvested for sonication. Then cell lysates including sheared genomic DNA were immunoprecipitated with the indicated antibody (anti-E2F1, anti-E2F6, anti-Rb, anti-HDAC1, and anti-HDAC2) or normal IgG to collect the DNA fragments that can bind with the interest proteins. The immune complexes were incubated with the salmon sperm DNA/protein A agarose slurry, and the collected DNAs were purified for further processes. The DNA library was prepared with TruSeq ChIP Library Preparation Kit (IP-202-1012; Illumina, San Diego, CA). Then the purified DNAs were sequenced using the Illumina HiSeq platform by the Genome Technology Access Center (GTAC) at the Washington University in St. Louis.
GEO Accession
GSM4455358