GSM2178500: WT rep3 T0; Aspergillus fumigatus; RNA-Seq
For RNAseq analysis, we used 2 to 5 μg of total RNA to purify polyadenylated mRNAs and construct the sequencing libraries using the TruSeq RNA Sample Prep Kit v2 Non-directional libraries were prepared using the TruSeq RNA Sample preparation kit v2 following the manufacturer’s instructions (Illumina). The nondirectional libraries were controlled on Bioanalyzer DNA1000 Chips (Agilent Technologies). 50-bp Single Read sequences were generated on the Hiseq2000 sequencer according to manufacturer’s instructions (Illumina). Sequences were demultiplexed using the Illumina pipeline (Gerald, included in CASAVA version 1.7) giving FASTQ formatted reads.
GEO Accession
GSM2178500