GSM2440914: T32_miRNA seq; Mauremys reevesii; miRNA-Seq
Eggs were incubated until they reached Greenbaum’s embryonic stage 16, at which point the embryonic gonads become responsive to ambient temperature. The AKG complexes, with three replicates at each incubation temperature, were collected using a stereoscopic microscope, and stored in liquid nitrogen for subsequent RNA extraction Total RNA was isolated from the frozen AKG complexes samples using Trizol reagent (TaKaRa, Dalian, China). The quantity and quality of the RNA were determined using a NanoPhotometer spectrometer (Implen Inc., Westlake Village, CA, USA). Then the RNAs of the three biological samples from the same incubation temperature were pooled together prior to constructing Illumina-indexed small RNA libraries.
GEO Accession
GSM2440914