GSM3336866: wt Col - cytoplasm - rep1 [mRNA-seq]; Arabidopsis thaliana; RNA-Seq
Total, Nuclear, and Cytoplasmic cell extracts were obtained as in the Cellular Fractionation protocol from Park et al., PMID: 15738428, with the following modification: Miracloth was substituted for using a 100 µm cell strainer. RNA was extracted using the Trizol LS protocol (Invitrogen) and enriched for poly(A)+ mRNAs using the NEBNext Poly(A) mRNA Magnetic Isolation Module. Libraries were prepared using the NEBNext Ultra II Directional RNA library prep kit and dual-indexed. Multiplexed libraries were sequenced on a HiSeq4000.
GEO Accession
GSM3336866