long paired-end sequencing of the Saccharomyces cerevisiae S288C
A Fosmid library was constructed. Pooled Fosmid DNA was sheared into 13-18 kb fragments and separated by pulse field gel electrophoresis (PFGE). Size selected DNA fragments were recovered by electroelution, end-repaired and ligated to the Ampicillin resistance gene label. Colonies transformed with the paired-end fragments containing the vector and the Amp tag were screened by chloramphenicol and ampicillin. Then, the vector was removed by I-SceI, and the paired-end fragments containing Amp were recovered and sequenced on the PacBio Sequel platform.