High-Throughput Analysis of Genomic Variability of Congenital Human Cytomegalovirus Involved in Neurological Symptoms
Amplicons were purified using magnetic beads (Agencourt AMPure XP) and fragmented using the Ion Xpress Plus DNA Fragment Library Preparation kit (Life Technologies). Barcodes adapters were ligated to fragment ends and 250 bp fragments were collected. The library was PCR amplified, then sequenced on the Ion Proton with the Ion Sequencing kit (Life Technologies). Base calling was performed with Torrent Suite Software version 5.0.2.