GSM4145053: mE12.5-mMZ-RNA1; Mus musculus; RNA-Seq
The embryonic hypothalamus areas were microdissected from coronal slices that were vibratome-sectioned  in ice-chilled ACSF to isolate VZ, medial MZ and lateral MZ regions and total RNA was harvested using RNeasy Micro Kit (QIAGEN, 74004). Then the total RNA was used for mRNA library construction using NEBNext® Ultra™ RNA Library Prep Kit for Illumina according to the Manuals. The mRNA libraries were sequenced on IlluminaHiseq 2500 platform with 100bp pair-end reads. The mRNA libraries were sequenced on IlluminaHiseq 2500 platform
GEO Accession
GSM4145053