DNA amplicon survey peatland microbial communities
Sequenced at the U.S. Department of Energy Joint Genome Institute (JGI, Walnut Creek, California, USA; now in Berkeley, California) using the protocol of Caporaso et al. (2012), with small modifications (see Tremblay et al., 2015; doi: 10.3389 /fmicb.2015.00771., and  Coleman-Derr et al., 2016; doi: 10.1111/nph.13697). PCR amplification utilized the primers 515F and 806R (Caporaso et al., 2012; doi:10.1038/nmeth.f.303) targeting the prokaryote 16S V4 region. Primers were fitted with Illumina sequencing adaptors and the reverse primer contained an 11bp barcode index. Sequencing used with 2 x 250 bp chemistry.