Monitor screen (Bed02)
Prior to DNA extraction, the cotton from the swab was removed, and DNA was extracted directly using the MasterPure Gram Positive DNA Purification Kit(Epicentre, Madison, WI, USA). Following extraction, DNA was quantified using a fluorometer (Qubit; Invitrogen, Carlsbad, CA). Barcoded PCR amplification was performed using universal primers flanking the highly variable V1-V2 region of the 16s rRNA gene sequence (Cai et al., 2013). PCR reactions were performed using 5X PCR Dye Master Mix II (GeneMark GMbiolab Co., Ltd., Taichung, Taiwan). All PCR cycles included an initial denaturation step at 94 C for 30 sec, an annealing step at 60 C for 20 sec and an extension step at 72 C for 30 sec. The amplification cycles were preceded by a one-time denaturation step at 94 C for 2 min prior to the first cycle and included a final step at 72 C for 10 min to ensure complete extension.    Individual barcoded PCR products were purified and then pooled with a total combined concentration of 1 mug (total volume: 50 mul). All barcoded PCR fragments were sequenced using an Illumina Miseq Desktop Sequencer at Yourgene Bioscience Co., Ltd. (Taipei, Taiwan).