The nodule transcriptome of Astragalus canadensis
Total RNA was depleted of ribosomal RNAs (rRNAs) with the combined use of the Ribo-Zero Magnetic Kits (Plant seed/root and Gram-negative bacteria) of Epicentre. Sequencing libraries were created with help of the NEBNext Ultra Directional RNA Library Prep Kit for Illumina from New England Biolabs.