GSM3336962: scPool1: pus7Δ scS100 +CMC; Saccharomyces cerevisiae; OTHER
Oligo pools (yeast, Twist Biosciences; human Custom Array) were PCR amplified, and in vitro transcribed into RNA, which was then gel purified, folded, and pseudouridylated with a pseudouridine synthase activity source. RNA was phenol:chloroform extracted from pseuoduridylation reactions, treated with 0.4 M CMC, followed by reversal of CMC modification in bicarbonate buffer. RT was performed, and truncated cDNAs were size selected, followed by circularization & PCR amplification.
GEO Accession
GSM3336962