GSM1440669: TAIL-seq exp #2 TUT7(ZCCHC6) KO; Homo sapiens; OTHER
Total RNAs were extracted from HeLa cells by TRIzol reagent (Invitrogen, 15596-018) and purified by RNeasy MinElute column (Qiagen, 74204) according to manufacturer’s instruction. TAIL-seq was carried out as described previously (Chang et al., 2014b). Briefly, 25-50 ug of total RNAs (>200 nt) were extracted by TRIzol (Invitrogen), purified by RNeasy MinElute column (Qiagen), and rRNA-depleted by using Ribo-zero kit (Epicentre). The RNAs were ligated to the biotinylated 3′ adaptor, and partially digested by RNase T1. The fragmented RNAs were pull-downed with streptavidin beads, phosphorylated at the 5′ end, and gel purified (500-1000 nt). The purified RNAs were ligated to the 5′ adaptor, reverse-transcribed, and amplified by PCR.
GEO Accession
GSM1440669