GSM2440233: Y128 WT Rep B; Saccharomyces cerevisiae; RNA-Seq
RNA was extracted with hot phenol protocol. DNA was digested with treatment of TurboDNase for 30 min at 37°C. rRNA was removed prior to library construction using Ribo-Zero Gold (yeast) kit (Epicentre). Library was constructed using EpiCentre Strand Specific ScriptSeq Kit using standard protocols. Epicentre ScriptSeq Index PCR Primers were used for indexing and multiplexing.
GEO Accession
GSM2440233