GSM4454660: E14226_input; Mus musculus; ChIP-Seq
Chromatin extraction and ChIP were performed as described in Kloet, S. L. et al. (doi:10.1038/nsmb.3248 ). For spike-in ChIP 50ng of sonicated Drosophila chromatin was added to each sample prior to IP Five ng/sample of ChIP were prepared for sequencing with the Kapa Hyper-prep Kit (Kapa Biosystems) using NEXTflex adapters (Bio Scientific) and amplified with ten cycles of PCR. Libraries were size-selected to obtain 300bp fragments using E-gel (Invitrogen) and sequenced on an Illumina NextSeq machine to obtain 75bp reads
GEO Accession
GSM4454660