Improved Yield of High Molecular Weight DNA Coincides with Increased Microbial Diversity Access from Iron Oxide Cemented Sub-Surface Clay Environments
Libraries were constructed by quantification of the bacterial 16S rRNA genes using the V1/V3 region for pyrosequencing with the Titanium™ 454 chemistry . The 16S rRNA gene specific region of the PCR primers FLXA_27F 5’ – cgt atc gcc tcc ctc gcg cca tca gAG AGT TTG ATC CTG GCT CAG – 3’ and FLX_B534R 5’ – cta tgc gcc ttg cca gcc cgc tca gcg caa cTY ACC GCG GCT GCT GG -3’ is shown in uppercase with the sequence tag and barcode in small case.