NCI Pediatric Malignancy: Sample Sample_MSKCC5002tumor1_T
After ribogreen quantification and quality control of Agilent BioAnalyzer, 1μg of total RNA (RIN between 5 and 7) underwent polyA selection and Truseq library preparation according to instructions provided by Illumina (TruSeqT RNA Sample Prep Kit v2), with 6 cycles of PCR.