GSM1982223: TCRab+ILTC1_1; Mus musculus; RNA-Seq
Cells were isolated from pooled tumors of 20- to 24-week-old PyMT mice. Tumors were prepared by mechanical disruption using a razor blade followed by treatment with 280 U/ml Collagenase Type 3 (Worthington Biochemical) and 4 μg/ml DNase I (Sigma) at 37°C for 1 h with periodic short vortexing. Digested tissues were mashed through 70-μm filters, and collected by centrifugation. Cell pellet was resuspended in 44% Percoll, layered on top of 66% Percoll (Sigma), and centrifuged at 1,900 g for 30 min without brake. Cells at the Percoll interface were collected, stained with antibodies and analyzed by flow cytometry. Cells were sorted using FACS Aria-II machines directly in Tri-Reagent (Sigma). RNA was prepared and complementary DNA (cDNA) libraries were amplified using the SMARTer RACE Amplification Kit (Clontech)
GEO Accession
GSM1982223