GSM4295082: AI_wt1 WT, replicate1; Bacillus subtilis; RNA-Seq
The samples were snap-frozen in liquid nitrogen and stored at -80°C until extraction. Frozen pellets were lysed by incubation with lysozyme (20 mg/ml) for 10 min at 37°C, and RNA was extracted using TRI Reagent (Sigma-Aldrich, T9424) according to the manufacturer's instructions. RNA levels and integrity were determined by Qubit RNA BR Assay Kit (Life Technologies, Q10210) and Tapestation, respectively. A total of 5 ug RNA was treated with RiboMinus™ Transcriptome Isolation Kit  (Invitrogen, K155004), according to the manufacturers' protocols. RNA quantity and quality post-depletion was assessed as described above. RNA-seq libraries were contracted with TruSeq RNA Library Preparation Kit v2 (Illumina RS-122-2001) according to the manufacturer's instructions. Libraries concentrations and sizes were evaluated as above, and were sequenced as multiplex indexes in one lane using the Illumina NovaSeq1500 platform. RNA-seq, paired-end
GEO Accession
GSM4295082