GSM3262248: WT-17; Mus musculus; RNA-Seq
RNA was isolated using the NucleoSpin kit (Macherey Nagel) or RNeasy Mini kit (Qiagene) RNA was isolated from Lats1-CKO and Lats2-CKO PyMT tumors. For each genotype, the corresponding wt littermate controls were used. For RNA-seq analysis, 500ng of total RNA was processed using the TruSeq RNA Sample Preparation Kit v2 protocol (Illumina) (Part # 15026495). Libraries were evaluated by Qubit and TapeStation. Sequencing libraries were constructed with barcodes to allow multiplexing of 7 samples per lane (3 wt tumors and 4 Lats-CKO tumors).
GEO Accession
GSM3262248