GSM2359424: myoblast_wt_3; Mus musculus; RNA-Seq
RNA was isolated from primary myoblast and 24-hour-differentiated myotube cells using Tripure (Roche) followed by the RNeasy Micro kit (Qiagen). Samples with RNA Integrity numbers >9 were used for sequencing. Ribosomal RNA was depleted from 1-5 μg total RNA using the RiboZero Gold kit (Illumina). Library preparation for the Illumina HiSeq 2000 was performed using the TruSeq RNA Sample Prep V2 kit (Illumina). Pooled barcoded libraries were sequenced to generate paired-end 101 bp reads. For the ABI SOLiD sequencing, libraries were prepared using the SOLiD Whole Transcriptome Analysis kit (Thermo Fisher Scientific). Pooled barcoded libraries were sequenced to generate paired-end 35 x 75 bp reads.
GEO Accession
GSM2359424