Illumina MiSeq data of sapovirus: intra-host temporal evolution
Viral RNA was extracted from 10% stool suspension using QIAamp viral RNA kit and the QIAcube (Qiagen, Hilden, Germany) and cDNA was synthesized using the SuperScript III First-Strand Synthesis System with random primers, according to the manufacturers protocol (Invitrogen, Carlsbad, California).  Sets of overlapping primers spanning the complete genome of closely related sapovirus GI.1 and GI.2 strains were designed and multiplex amplicons  were generated following the method of Quick et al, Nature protocols, 2017. Library was prepared using Nextera XT DNA library preparation kit and data was analyzed using iVar