Illumina sequencing data of Cupriavidus metallidurans MSR33
MSR33 cells were grown overnight in MM284 liquid media and genomic DNA was extracted by using the Quiagen QIAamp DNA Mini Kit (ThermoFisher, Aalst, Belgium) following instructions of the manufacturer. DNA quantity and quality were measured using a DropSense (Trinean). Genome sequencing was performed on a HiSeq 2500 apparatus (Illumina, San Diego, USA) using 2 x 250 bp paired-end reads. The reads were trimmed using the Trimmomatic tool [Bolger et al., 2014] and their quality assessed using in-house Perl and shell scripts in combination with SAMtools [Li et al., 2009], BEDTools [Quinlan, 2014], and Burrows-Wheeler alligner with maximum exact matches (bwa-mem) [Li, 2013].