GSM3108348: P4_miRNA-seq; Rattus norvegicus; miRNA-Seq
The bone around the implant was dissected with a margin of 1 mm and the surrounding muscle tissues were thoroughly removed.Then the remaining bone specimens were grinded into powder while submerged in liquid nitrogen and total RNA was isolated with QIAGEN miRNeasy Mini Kit (QIAGEN, USA). Small RNA sequencing libraries were prepared from the abovementioned total RNA using TruSeq Small RNA Sample Prep Kit (Illumina, USA). Briefly, the RNA fragments were ligated to 5'- and 3'-adapters using T4 RNA Ligase 1 and 2, respectively, before being reverse-transcribed with miScriptII RT-Kit (Qiagen, Germany) and then amplified. The resultant cDNA fragments were electrophoresized on a 4% agarose gel. Bands between 140 and 160 bp were excised and the DNA was extracted by MinElute Gel Extraction Kit (Qiagen, Germany). Library constructs were then sequenced on the HiSeq4000 Illumina platform (Illumina, USA) to generate 50-bp single-end reads.
GEO Accession
GSM3108348