RNA-Seq of Human respovirus 3: cultured virus
RNA was extracted using the Zymo Quick-Viral RNA kit and treated with Turbo Dnase. Double-stranded cDNA was constructed with random hexames and Superscript IV, followed by second strand synthesis with Sequenase polymerase V2.0. The resulting cDNA was cleaned with the Zymo Clean & Concentrator kit. Libraries were constructed with the Nextera XT kit and sequenced on a 1x192bp Miseq run.