piD1 GFP cell
monkey blastocysts after injection of dESCs were cultured on 8-well IbiTreat -plates (IB-80826; Ibidi GmbH) with in vitro culture media in 5% CO2 incubator. For the first 24 h, the GFP single cell were isolated.embryos were digested into single cells following treatment with 0.5% Trypsin. Cells were grouped with GFP negative or positive using inverted fluorescence microscope. GFP+ injected cells and GFP- host embryonic cells were collected for gene expression analysis, respectively. To exclude GFP cell marker expression used in PCR may come from recipient embryonic cells, single GFP+ cells were picked up under fluorescence microscopy, respectively. The contaminations of the host embryonic cells were excluded before performing RNA isolation. cDNAs synthesis and amplification were performed with Discover-sc WTA Kit V2 (Vazyme N711, China) according to the instruction manual. The qualities of the cDNAs were evaluated by Bioanalyzer 2100.