GSM1444700: mEpiSCbulk 2; Mus musculus; ChIP-Seq
use microfludic chip to do ChIP The reagents for library preparation were provided by Illumina and were used according to the manufacturer’s protocol with appropriate modifications as described below. We purified DNA from the ChIP assay by AMPure XP beads and eluted the DNA by 20µl Resuspension Buffer. The DNA suspension was concentrated from 20µl to 5µl by evaporation. Then, 4µl End Repair Mix and 1µl Resuspension Buffer were added to the 5µl cleaned up DNA suspension before incubating at 30°C for 30 minutes and inactivating at 75°C for 15 minutes for end repairing. Adenylation  of  3’  ends  is  performed  by  adding  8.4µl  thawed  A-Tailing  Mix  and  1.6µl Resuspension Buffer, incubating at 37°C for 30 minutes. We ligated adapters by adding 1.7µl of DNA ligase Mix, 3.1µl Resuspension Buffer and 0.2µl Adapter Index. 30°C for 10 minutes is the duration for adapter ligation. We added 1µl DNA which contains 100 ng of 5kb sized plasmid DNA as carrier, and then purified DNA twice by AMPure XP Beads.
GEO Accession
GSM1444700