GSM4454174: mH3K9me3_WT_new; Mus musculus; ChIP-Seq
ChIP assays were performed using a ChIP assay kit (Upstate Biotechnology, Cat No: 17-295) per the manufacturer's instructions. Briefly, cells were double crosslinked with 10 mM DTBP for 10 min at room temperature, followed by incubation in 1% formaldehyde for 15 min in a 37 °C water bath. Nuclei were extracted and sonication was perfromed to generate 200–500 bp genomic DNA fragments. Chromatin complexes were immunoprecipitated with the related antibodies and Dynabeads Protein A/G. The precipitated DNA was purified using DNA clean and concentrator (Zymo Research). Libraries were prepared using KAPA Hyper Prep Kit (Kapa Biosystems, Cat No:  KK8502)
GEO Accession
GSM4454174