SINV in Culex pipiens
Cx. pipiens mosquitoes were infected with Sindbis virus by intra-thoracic injection. Two days post infection, RNA was extracted from the mosquitoes using Isol-RNA lysis reagent. Small RNAs were isolated by gel-electrophoresis and deep-sequencing libraries were prepared using Illumina’s Truseq small RNA preparation kit. Small RNA libraries were then sequenced on a Illumina Hiseq2500 system.