GSM2440672: sir4[delta]-KO rep2; Kluyveromyces lactis; RNA-Seq
Hot phenol treatment was used to harvest total RNA. Turbo Dnase (Life technologies) was used to remove DNA. Samples were concentrated using a Qaigen RNA column. RNA samples were processed for sequencing using the TruSeq non-stranded RNA Library Prep Kit (Illumina), and 50 bp single end sequencing was performed on an Illumina HiSeq 2500 machine at SUNY Buffalo gemonics core facility.
GEO Accession
GSM2440672