Illumina HiSeq 2000 paired end sequencing; Genome-wide occupancy of Rif1FH in  mouse ESC
Genome-wide occupancy of Rif1FH in  mouse ESC
3 independent embryonic stem cells (ESCs) lines carrying an homozygous allele encoding for an N-terminal FLAG-HA2 Rif1 were used for ChIP seq to determine the genome-wide Ri1f occupancy. Littermate wild type cells were used as negative controls. ChIP-seq libraries were prepared with NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina (NEB#E6240S/L), except that bead purification was employed (Agencourt AMPure XP beads, Beckman Coulter A63881). Size selection was performed on 2% agarose gels (E-Gel Invitrogen G6610-02). Generally 13 PCR cycles for 10ng starting material were applied. Libraries were barcoded with the NEBNext Multiplex Oligos for Illumina (Index Primers Set 1, NEB#E7335S/L).
Experimental Factor: immunoprecipitate
IP
LIBRARY_STRAND
not applicable