Inowroclaw_Fall_1
100 mg of frozen plant tissue of roots and shoots were ground in liquid nitrogen.  Total RNA was extracted using TriPure reagent (Roche) and following the modified single-step RNA isolation method developed by Chomczynski and Sacchi (1987). DNase treatment was carried out using DNase I (Thermo Scientific). cDNA was synthesized from 1  g of RNA using the TruSeq RNA Sample Preparation v2 HS workflow.