Reprogramming day 6 (Phase II-iNSC)
scRNA-seq libraries were generated using the Chromium Single Cell 3 Reagent Kit v2 (PN-120267, 10X Genomics), Chromium Single Cell A Chip Kit (PN-120236, 10X Genomics) and Chromium Single Cell i7 Multiplex Kit (PN-120262, 10X Genomics). The cell viability was estimated to be over 90% by trypan blue staining under the microscope. Cells were diluted to 2105 ~ 2106 cells/mL with 0.04% BSA in DPBS and loaded on the Chromium microfluidic platform, aiming to capture 3,000 cells per each channel. Subsequent library preparation was performed according to the manufacturers instructions provided. Libraries were sequenced on an Illumina HiSeq 4000 platform (2100bp).