GSM1982235: wt_TCC_st10.5_rep2; Xenopus laevis; OTHER
Embryos were fixed in 1% paraformaldehyde in PBS for 30 minutes at 22 degrees and fix was quenched with 0.125M glycine. TCC was performed as described (PMID: 22198700) with the following modifications: Tissue was thawed, rinsed in PBS, and lysed directly in 250 ul ice-cold wash buffer with SDS added (50 mM Tris.HCl pH=8.0, 50 mM NaCl, 1 mM EDTA, 0.56% SDS) rather than using lysis buffer. Following sonication of ligated fragments, we polished and adenylated ends (NEB End-It and A-tailing modules) and ligated Truseq v2 adapters (Illumina).
GEO Accession
GSM1982235