GSM3039849: ZmARF13 DAP-seq; Zea mays; OTHER
Genomic DNA was purified using a standard phenol:chloroform extraction protocol. GST-ARF fusion proteins were purified from Ecoli using GSH-sepharose. GST-HALO negative control protein was expressed using Promega's TNT wheatgerm expression system. DAP-seq is a transcription factor binding site discovery assay that couples affinity-purified transcription factors with next-generation sequencing of a genomic DNA library (O'Malley et al. Cell 2015, Bartlett et al. Nature methods 2017). Please let me know if you need further information. The O'Malley reference is the first publication of DAP-seq.
GEO Accession
GSM3039849