GSM2088231: Stau_rep3; Caenorhabditis elegans; miRNA-Seq
Animal were collected and flash frozen in liquid nitrogen. Total RNA was extracted using Trizol reagent (Invitrogen). 20 mg of total RNA for each sample was used to isolate small RNA populations. RNA samples were run on a 15% PAGE/urea gel and small RNA populations were isolated from the gel with sizes ranging from 18–26 nucleotides (nt) Sterling, C. H., I. Veksler-Lublinsky, and V. Ambros, 2015. An efficient and sensitive method for preparing cDNA libraries from scarce biological samples. Nucleic Acids Res. 43: e1.
GEO Accession
GSM2088231