GSM4021913: SCC1CTX_replicate1; Homo sapiens; ATAC-seq
For nuclei isolation, cells were scraped, washed in PBS and lysed (lysis buffer: 10mM Tris-Cl, pH7.4; 10mM NaCl; 3mM MgCl2; 0.05% IGEPAL). Nuclei tagmentation and adapter ligation by Tn5 was performed using the Nextera DNA Sample Preparation kit (Illumina) and followed by purification with MinElute PCR Purification kit (QIagen), according to manufacturers' instructions. Transposed DNA fragments were amplified for 5 cycles using the NEBNext Q5 HotStart HiFi PCR Master Mix with regular forward and reverse barcoded primers. Additional number of amplification cycles were determined by quantitative-PCR using the NEBNext HiFi Master Mix, SYBR Green I (Applied Biosystems) and Custom Nextera Primers. The final product was purified with MinElute PCR Purification kit (QIagen) and quality checked on 2100 Bioanalyzer (Agilent).
GEO Accession
GSM4021913