Novel detection of xenobiotic resistance related P450 genes and comparative transcriptome analysis of Myzus persicae
The transcriptomes of M. persicae sensu stricto (green morph), M. persicae sensu stricto (red morph) and M. persicae nicotianae (green morph).   Apterous adult aphids three strains were gently collected into microcentrifuge tubes and rapidly  frozen in liquid nitrogen before storage at -80 °C.  Total RNA was extracted from aphids using TRIzol (Invitrogen, USA) according to the manufacturer's instructions and treated with RNase-free DNase I (Takara, China). Poly(A) mRNAwas isolated using oligo-dT beads and treated with a fragmentation buffer. Cleaved RNA fragments were transcribed into first-trand cDNA followed by second-strand cDNA synthesis using DNA polymerase I and treatment with RNaseH. Double-tranded cDNA was further subjected to end-repair and adaptor ligation. Adaptor-ligated fragments.were selected according to size, and the desired range of cDNA fragments were excised from the gel. PCR was performed to selectively enrich and  amplify selected fragments. The cDNA libraries were sequenced using the Illumina Solexa platform.