Illumina HiSeq 2000 paired end sequencing; PRR14L mutations are associated with chromosome 22 acquired uniparental disomy, age related clonal hematopoiesis and myeloid neoplasia
PRR14L mutations are associated with chromosome 22 acquired uniparental disomy, age related clonal hematopoiesis and myeloid neoplasia
Five samples were selected with acquired uniparental disomy of chromosome 22 and various hematological conditions, either CMML, MDS/MPN or age related clonal hematopoiesis. For samples E4051 and E6523, both tumor (peripheral blood leukocyte) and constitutional (cultured T-cell) DNA was extracted. T-cells were isolated from anticoagulated peripheral blood using CD3 MicroBeads and cultured using a T-cell Activation/Expansion kit (Miltenyi Biotec, Bisley, UK). For samples ULSAM1182, ULSAM1242 and ULSAM1356 DNA was extracted from the tumour only (peripheral blood leukocyte). Samples E4051 and E6523 were prepared for exome sequencing using the Agilent SureSelect kit (Agilent Technologies, Palo Alto, CA, USA) (Human All Exon 50 Mb). Whole exome sequencing of samples ULSAM1182, ULSAM1242 and ULSAM1356 was performed separately in Stockholm, Sweden.
Experimental Factor: cell type
T cell
Experimental Factor: disease
myeloid neoplasm with eosinophilia