GSM3037258: Type3b OC 2; Mus musculus; Bisulfite-Seq
Harvested oocytes were washed three times in 0.5% BSA-PBS solution and transferred into the lysis buffer. After lysed for 3h at 50°C, naked genomic DNA were relesed. The released naked DNA was digested by MspI(Fermentas,ER0541),The digested DNA was then end-repaired and tailed with an extra A to the 3'blunted ends.Illumina standard premethylated indexed adaptors were ligated with the dA-tailed DNA fragments. Bisulfite conversion was then performed using the MethyCode bisulfite conversion kit (Invitrogen,MECOV-50) followed by two rounds of PCR enrichment.After PCR enrichment, DNA fragments between 200 and 700bp were size-selected by a 12% native polyacrylamide TBE gel.
GEO Accession
GSM3037258