RNA-Seq of sus scrofa PRRSV infected SIGLEC Knock-out macrophages
Deep sequencing was only performed on the SIGLEC1-/- (4 biological replicates) and SIGLEC1+/+ (4 biological replicates).  Total RNA (2.5 g) was transported to the University of Missouri DNA Core Facility.  Libraries were prepared for transcriptome sequencing on the Illumina HiSeq 2500 as described previously (Whitworth et al., 2015, Spate et al., 2014).  Briefly, cDNA was fragmented by using a Biorupter UCD-200TM (Diagenode, Liege, Belgium).  One microgram of the fragmented DNA was used to generate the sequencing libraries by using Illuminas TruSeq DNA sample prep Kit (Illumina, San Diego, CA).  Eight samples to a lane were barcoded, multiplexed and loaded at an 8 pM concentration.  A single read collecting 100 bases, and 6 bases of an index read, were performed with Illumina TruSeq SBS version 3 chemistry.  Demultiplexing and fastq files were generated by using CASAVA 1.8.2 (Illumina).   Transcriptome comparisons between SIGLEC1-/- and SIGLEC1+/+ PAMs.  SIGLEC+/- were not evaluated, but raw sequence is being made publicly available.