GSM3038974: C9orf72_C9-ES-1; Homo sapiens; Bisulfite-Seq
DNA extracted under native conditions First PCR products were purified using SPRI beads (X1), and then a second PCR was performed to add Illumina adapters with the indices. PCR products were cleaned again using SPRI beads (X1) and eluted in 25 ul elution buffer. To confirm the length and purity of the library; 1 µl was loaded onto an Agilent TapeStation 2200 using the D1000 ScreenTape assay (Agilent Technologies, Santa Clara, USA). Library concentration was measured using the Qubit® dsDNA HS (High Sensitivity) Assay Kit. The libraries were normalized to 4 nM and loaded on a Miseq (Illumina, USA) using the MiSeq Reagent Kit v2 (500 cycles) with the following conditions: 250x2 in final concentration of 8pM and 20% phiX spike-in.
GEO Accession
GSM3038974