Dynamic Evolution of Clonal Epialleles Revealed by Methclone
Acute myeloid leukemia (AML) de-identified patient samples at two time points (diagnosis and relapse stages), enriched for myeloblast cells, were used in the experiments, and these samples were also compared to five normal bone marrow samples.  Institutional review board approval was obtained at Weill Cornell Medical College, and at the Royal Adelaide Hospital, and this study was performed in accordance with the Helsinki protocols.  DNA was extracted using standard techniques and ERRBS library preparations were performed as previously described(Akalin, Garrett-Bakelman et al. 2012).  Libraries were sequenced on a HiSeq2500 Illumina machine using 75 bp single-end reads to an average depth of 79X per covered CpG.