GSM1162047: methyl-C-seq_DKO_rep2; Mus musculus; Bisulfite-Seq
Genomic DNA was extracted from mESCs and was spiked in with unmethylated lambda DNA (Promega). The DNA was fragmented by sonication. The DNA was fragmented by sonication. Purified DNA fragments were end-repaired. After A-tailing reaction, fragments were ligated to paired-end cytosine-methylated adapters provided by Illumina. Size-selected adaptor-ligated DNA was treated with sodium bisulfite using the EZ DNA methylation-Gold Kit (Zymo Research). The resulting DNA molecules were enriched by PCR, purified, and sequenced following standard protocols from Illumina.
GEO Accession
GSM1162047