Sample_8
Seedlings of the model plant Arabidopsis thaliana (ecotype Col-0) were grown on 0,5 x MS (0,5 % MES, no sucrose) under a long-day photoperiod (16h of light), with a light intensity of 120-140 E, and at a temperature of 22 C.          5 day after seed sowing, Arabidopsis thaliana seedlings were inoculated with 10L of Pseudomonas simiae WCS417r at 2.106 CFU.mL-1 (in 10 mM MgSO4 solution) in physical contact with their roots, or mock-treated (10 mM MgSO4 solution). The plates were sealed with two layers of parafilm and transferred back to the plant growth chamber. Seven day post inoculation, the root and shoot tissues were harvested for total extraction and genome-wide gene expression analysis by RNA-sequencing. This experiment was performed in three biological replicates (pool of 30-40 seedlings), each from an independant experiment.         Total RNA was extracted using a Phenol-chloroform-LiCl extraction procedure adapted from Box et al. (2011). RNA samples were resuspended in RNAse-free water, quantified using a Qubit 2.0 fluorometer and their integrity were checked by using the MultiNA microchip electrophoresis system.         RNA libraries were prepared with polyA selection using the NEBNext Ultra RNA Library Prep kit for sequencing on an Illumina HiSeq4000 platform by the company Genewiz.