GSM1439610: Prdm16-overexpressing Neural Stem Cells; Mus musculus; Bisulfite-Seq
Genomic DNA was isolated with QIAamp DNA Mini Kit (QIAGEN) a. Genome DNA were cut by Restriction Enzyme; b. DNA-end repair, 3'-dA overhang; c. Select the 40-220bp fragment; d. Bisulfite treatment by ZYMO EZ DNA Methylation-Gold kit; e. PCR amplification; f. Qualified library for sequencing.
GEO Accession
GSM1439610