Illumina HiSeq 2000 sequencing; Chromosome conformation capture in Rif1 null pMEFs
Chromosome conformation capture in Rif1 null pMEFs
Chromosome conformation capture was performed starting from 1x107 cells, cross-linked in 2% formaldehyde. Rif1F/F Rosa26Cre-ERT2 pMEF synchronized in G1 have been treated with tamoxifen to activate the Cre and induce the deletion (pMEF_Rif1 ko). Rif1+/+ Rosa26Cre-ERT2 pMEF were treated in parallel  (pMEF_Rif1wt) Primary restriction digest was performed by incubation with HindIII, while for the secondary digest we have chosen the 4-bp cutter DpnII.
Experimental Factor: genotype
Rif1+/+
Experimental Factor: viewpoint
LL
LIBRARY_STRAND
not applicable