GSM1446330: CTCF_Patski_ChIPSeq; Mus musculus x Mus spretus; ChIP-Seq
ChIP was performed using fixed chromatin from mouse brain or cell lines. DNA from ChIP pull-down chromatin fractions were de-crosslinked and purified using QIAquick PCR purification kit. Genomic DNA was prepared from an aliquot of the same batch of nuclei from F1 brain Sequencing libraries were prepared each from four pooled independent PolIIS5p or CTCF ChIP samples from Patski cells or from Xist mutant F1 brain, using the Illumina seq sample prep kit. Size selection of the library was done by gel excision and purification of DNA in the 300-600bp range. Control sequencing library was prepared from genomic DNA prepared from F1 brain.
GEO Accession
GSM1446330