GSM4024051: Disc-pSS5; Homo sapiens; RNA-Seq
Plasmacytoid dendritic cells were isolated from peripheral blood mononuclear cells with MACS using the BDCA-4+ isolation kit (Miltenyi) according to manufacturer's instruction. Cells were lysed in RLTplus buffer (Qiagen) supplemented with beta-mercaptoethanol (1%). Total RNA was purified using AllPrep Universal Kit (Qiagen), according to the manufacturer's instructions. RNA integrity was measured by capillary electrophoresis using a Bioanalyzer (Agilent), and the RNA 6000 Nano Kit (Agilent Technologies); all samples had RIN-score >7.0. RNA libraries were prepared for sequencing using standard Illumina protocols