WGS of Escherichia coli 98NK2
A paired-end library was prepared using either the NxSeq AmpFREE Low DNA Library Kit (Lucigen) with 250-bp read length and sequenced with the MiSeq Reagent kit v2 500-cycle (Illumina), or the KAPA HyperPlus DNA kit (Roche) with 300-bp read length and sequenced with the MiSeq Reagent kit v3 600-cycle (Illumina), following the manufacturers guidelines. Briefly, genomic DNA was fragmented, end-repaired, A-tailed, and ligated with adaptors compatible with Illumina sequencing, followed by PCR amplification of the DNA inserts and library clean-up. Finally, the libraries were normalized and pooled for sequencing.