GSM4021877: BM_LTHSC_RNA-Seq_Rep2; Mus musculus; RNA-Seq
5 stages of hematopoietic stem and progenitor cells were sorted from mouse embryos or adult bone marrow. Total RNA samples were isolated using Rneazy Micro Kit (Qiagen). Libraries were constructed using NEBNext Ultra RNA Library Prep Kit for Illumina according to manufacturer's instructions including the optional double size selection procedure using Agencourt AMPure XP beads. Prepared libraries were quantified with dsDNA HS Kit for Qubit and the size distribution was assessed using High Sensitivity DNA Kit for Bioanalyzer. Libraries were sequenced on Illumina HiSeq2500 in paired-end mode with the read length of 100 nt.
GEO Accession
GSM4021877