GSM3639560: Drop-Seq_wing.disc_rep1_Lib3; Drosophila melanogaster; RNA-Seq
Larvae were dissected in Schneider's medium in batches of 5 animals (to prevent hypoxia) and transferred into a tube containing Schneider's medium on ice for a maximum time of 30 minutes. To isolate single cells TrypLE (10x) was added and the tissue incubated for 15 minutes in a water bath at 37°C , with gentle mixing every 5 minutes. Schneider's medium was then added to the loosened tissue pellets, followed by gentle mechanical dissociation using a P1000 pipette. The cell suspension was then passed through a 10 μM cell strainer to remove undigested tissue and cell clumps. Drop-Seq libraries were prepared according to Macosko et al., 2015 (PMID 26000488)  following the detailed online protocol (Drop-seq-Protocol-v1.0-May-2015)
GEO Accession
GSM3639560