GSM2178307: pair fed control with lipopolysaccharide injection 4hr prior to sacrifice, replicate 1; Mus musculus; RNA-Seq
At the time of sacrifice, kidneys were collected and cortex sections were separated and snap frozen.  Tissues were stored at -80° C and then incubated in RNAlater-ICE (Life Technologies, Carlsbad, CA) at -20° C for 72 hours prior to RNA extraction.  RNA was isolated using the mirVana kit (Life Technologies, Carlsbad, CA) according to manufacturer’s instructions for total RNA isolation.  RNA quality was evaluated by Nanodrop ND-1000 (Thermo Fisher Scientific, Grand Island, NY) and Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA) analysis. Libraries were prepared using the TruSeq Stranded Total RNA LT Sample Prep Kit-Set A (Cat # RS-122-2301) with Ribo-Zero Gold.
GEO Accession
GSM2178307