Amplicon of SB insertion sites in Ptch+/-/Math1-SB11/T2Onc mice : Post-treatment Frontal Lobe Right, Reverse
Genomic DNA was isolated and purified from mouse tissues with a PureLink genomic DNA extraction kit (Invitrogen). Libraries for illumina Hi-Seq sequencing were prepared as described previously (Brett et al., 2011) with minor modifications. Briefly, 2_g of gDNA were digested and ligated to the adapters, after a BamHI digest to eliminate the untransposed copies of the concatamer, an enrichment PCR followed by a barcoding PCR were performed (Brett et al., 2011). The barcode PCR was modified to incorporate a paired-end (PE) sequencing adapter for paired end sequencing, the sequence of the PE adapter was: CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCTTAGGGCTCCGCTTAAGGGAC. Libraries were purified and pooled as previously described (Brett et al., 2011) and sequenced on an Illumina Hi-Seq 2000.