GSM2359395: 3023-Naïve-CD8; Homo sapiens; RNA-Seq
Cells were lysed in Trizol reagent (Ambion) and total RNA isolate  d using DirectZol kit   (Zymo) with on-column DNA digestion according to manufacturers instr  uctions. RNASeq libraries were prepared   using the TruSeq sample prep kit (Illiumina) RNAseq for 50bp paired end runs on HiSeq platform. To minimize variation, libraries were multiplexed, and run as a batch on two flow cells (noted as 781 and 782, respectively). Results from the two flow cells were combined for later analysis.
GEO Accession
GSM2359395