GSM2948466: mESC - E2F4KO (J1-23); Mus musculus; RNA-Seq
Cells were grown at low density for four days. RNA extraction was performed using the RNeasy micro kit (Qiagen 74004). Libraries were prepared using the TruSeq Stranded mRNA Library Preparation Kit (Illumina RS-122-2101). Four wild-type and four E2F4KO mESC lines were sequenced on a HiSeq4000, generating on average 30 million paired-end (2x76) mapped reads per line. Two of the wild-type lines (J3.3 and J3.14) were expanded from clones that had retained E2F4 protein expression following transfection with a gRNA against E2F4, and the other two wild-type lines (C1 and C2) were obtained following transfection of an empty pX330 vector. Similarly, four TKO and four QKO lines were sequenced on a separate run of a HiSeq4000, also generating on average 30 million paired-end (2x76) reads per line.
GEO Accession
GSM2948466