GSM4295270: ERCC3WT/WT irofulven_biological replicate 3; Homo sapiens; RNA-Seq
Fresh frozen tumor tissues were subsequently preserved in RNAlater (Invitrogen) and RNA was extracted using the RNeasy Midi Kit (Qiagen). After RiboGreen quantification and quality control by Agilent BioAnalyzer, 0.99-1ug of total RNA with an RNA integrity number varying from 3.8-10 underwent ribosomal depletion. Library preparation was performed using the TruSeq Stranded Total RNA LT Kit (Illumina catalog # RS-122-1202) according to instructions provided by the manufacturer with 8 cycles of PCR. Samples were barcoded and run on a HiSeq 4000 in a 100bp/100bp paired end run, using the HiSeq 3000/4000 SBS Kit (Illumina).
GEO Accession
GSM4295270