GSM4146037: 22-T52-TTR-CII-M; Homo sapiens; OTHER
Biopsies were resuspended in 180 μl TET buffer and 20mg lysozyme. Samples were incubated for 1h at 37ºC and vortexed with 25 μl Proteinase K before incubating at 56ºC for 3h. Buffer B3  was added followed by a heat treatment. Two washing steps were performed before eluting DNA, concentrations and purity were checked using NanoDrop One (Thermo Fisher Scientific) V3-V4 regions of the 16S rRNA gene were amplified using standard protocols  with forward and reverse primers 341F-785R. Following the manufacter's protocol with a pooled samples in paired-end modus (PE275) Paired sequencing on a MiSeq system (Illumina, San Diego, CA)
GEO Accession
GSM4146037