GSM4090249: pMV261_stat_rep2; Mycobacterium tuberculosis; RNA-Seq
Bacterial cultures were grown up to the stationary phase,  rapidly cooled on ice, centrifuged, and total RNA was isolated by phenol-chloroform extraction after cell disruption with Bead Beater. After isolation, RNA was treated with Turbo DNase (Life Technologies, Carlsbad, CA, USA) to remove traces of genomic DNA, and purified with the RNeasy mini kit (Qiagen, Venlo, Netherlands). RNA samples were depleted of 16S and 23S rRNA using RiboMinus Transcriptome Isolation Kit, Bacteria (ThermoFisher). Sequencing libraries were generated using the resulting ribosomal transcript-depleted RNA and NEBNext Ultra II Directional RNA Library Prep Kit (NEB) according to the manufacturers' protocol.
GEO Accession
GSM4090249