GSM3263203: DND41_WO_RNAseq_rep2; Homo sapiens; RNA-Seq
Cells from GSI and washout conditions were washed with 1 x PBS (Corning, cat# 21031CV) and lysed with 350 μl RLT Plus buffer (Qiagen) supplemented with 10% 2-mercaptoethanol (Sigma, cat# M6250), vortexed briefly, snap-frozen on dry ice, and stored at -80°C. Subsequently, total RNA was isolated using the RNeasy Plus Micro Kit (Qiagen, cat# 74034). RNA integrity numbers were determined using TapeStation 2200 (Agilent), and all samples used for RNA-seq library preparation had RIN numbers greater than 9.5. 800 ng of total RNA was used and libraries were prepared using the SMARTer Standard Total RNA Sample Prep Kit-HI Mammalian (Clontech, cat# 634873). Libraries were single-end sequenced (75 bp) on a NextSeq 550.
GEO Accession
GSM3263203