Sp. rotten
The genomic DNA in three replicates was extracted from 10 to 30 mg of sponge tissue or primmorphs. Each experiment involved 3 primmorphs in 3 replicates using TRIzol LS reagent (Invitrogen, Ambion, USA) according to the manufacturers instructions. The universal bacterial primers 518F and 1064R (Klindworth et al. 2013) were used to amplify the V4V6 hypervariable region of the bacterial 16S rRNA gene by PCR using the following program: 3 min for at 96 C; 30 cycles of 94 C for 20 s, 55 C for 20 sec., and 72 C for 1 min, with a final 10 min incubation at 55 C