DNAseq of Paramecium bursaria HK1 :Whole cell
The aposymbiotic strain of HK1 in the early stationary phase was starved for 2 days and collected by water pipe equipped with 11m pore size nylon membrane (NY1102500, Millipore). The cells were washed with modified Dryls solution twice and lysed by equal volume of 0.25 M TCMS buffer (10 mM Tris-HCl pH 8.0, 2 mM CaCl2, 8 mM MgCl2, 0.25 M sucrose) with 0.3% (w/v) NP-40. The DNA was extracted by the QIAGEN Genomic-tip 20/G (Cat No.10223, QIAGEN).