GSM1981896: pfbloodrnaS4; Plasmodium falciparum; RNA-Seq
Illumina libraries were prepared and sequenced at the HudsonAlpha Institute for Biotechnology, using an Illumina HiSeq2000 sequencer. Standard directional RNA-seq library construction, 50 bp paired end reads with ribosomal reduction (RiboMinus™ Eukaryote Kit, Ambion®). Total RNA was extracted from fresh mosquito tissues (~50 midguts and ~ 50 salivary glands) using the mirVana™ RNA Isolation Kit (Ambion®) according to the manufacturer’s protocol.  Nuclei extraction was performed on formaldehid fixed tissues (~50 midguts and ~ 50 salivary glands) followed by chromatin immunoprecipitation according to previously published protocols.
GEO Accession
GSM1981896