GSM4707951: Sample 2 untreated; Homo sapiens; RNA-Seq
RNA was purified using the Qiagen RNeasy kit as per manufacturer's protocol Barcoded Ion Ampliseq Transcriptome Gene Expression sequencing libraries were constructed. Libraries were sequenced on an Ion Torrent Proton sequencer to generate quantitative gene expression data for >20,000 RefSeq gene transcripts. Sequencing reads were mapped to the AmpliSeq Transcriptome Gene Expression target regions and quantified with the ampliSeqRNA plugin for Ion Proton System. We removed duplicated sequences from the FASTA file. We used the uniquely mapping reads option of the plugin. We modified the bed file locations for 166 amplicons which were inconsistent with the primer position of these amplicons in the corresponding FASTA sequence. RNA AmpliSeq
GEO Accession
GSM4707951