GSM3262696: X3D_B; Mus musculus; RNA-Seq
RNA was harvested using Trizol reagent and extracted with phenol:chloroform:isoamyl alcohol. The first step in the workflow involves purifying the poly-A containing mRNA molecules using poly-T oligo-attached magnetic beads. Following purification, the mRNA is fragmented into small pieces using divalent cations under elevated temperature.We then quantified the PCR yield by Qubit and pooled samples together to make a single strand DNA circle (ssDNA circle), which gave the final library.
GEO Accession
GSM3262696