Schistosoma mansoni ChIP-Seq Male Adults Unbound replicate 2
ChIP library constructions and sequencing were performed at the sequencing facilities of Montpellier GenomiX (MGX, France). The TruSeq® ChIP sample preparation kit (Illumina Inc., USA) was used according to the manufacturer's recommendations on 30 ng of DNA per condition. Briefly, DNAs were blunt ended and adenylated on their 3' ends. Illumina's indexed adapters were ligated to both ends. Ligated DNA were enriched by PCR and size separated by electrophoresis. Size selection was performed at 400 base pairs (bp). The quantitative analysis of the DNA library was carried on Agilent High Sensitivity chip and qPCR (Applied Biosystems 7500, SYBR Green). The sequencing was performed on a HiSeq2500.