GSM3262105: hyphae_24 hrs_benzo(a)pyrene rep 2; Aspergillus nidulans FGSC A4; ssRNA-seq
Hyphae samples were collected, squeezed dried and flash frozen in liquid nitrogen. Total RNA was extracted using Trizol, silica/zirconia beads in a minibeadbeater, and precipitation with isopropanol. mRNA was extracted from total RNA using polyA selection, followed by RNA fragmentation Strand specific library was constructed by first-strand cDNA synthesis using random primers, followed by sample cleanup and the second-strand synthesis using DNA Polymerase I and RNase H. A single 'A' base was added to the cDNA fragments followed by ligation of the adapters. Final cDNA library was obtained by further purification and enrichment with PCR, and the quality was checked using Bioanalyzer 2100
GEO Accession
GSM3262105