GSM1443916: control rep2; Caenorhabditis elegans; RNA-Seq
At least 100 adult worms per condition were collected in 0.1 M NaCl and mRNA was isolated using the Trizol reagent (Life technologies) according to the manufacturer’s protocol. Sample preparation was performed essentially as described previously (Mortazavi et al., 2008). First-strand cDNA synthesis was performed using SuperScript VILO cDNA synthesis kit (Invitrogen) according to the manufacturer’s protocol using 50 ng/µl random hexamers as primers. To preserve strand information, dUTP was used instead of dTTP for second strand synthesis. Libraries were prepared using standard Illumina protocols.
GEO Accession
GSM1443916