GSM3401046: PP006_L6; Homo sapiens; MeDIP-Seq
Samples of villous parenchyma were taken from four quadrants between the chorionic and basal plate.  Samples were placed immediately into RNAlaterTm (Ambion, Inc., #AM7021) and stored at -80°C.  Genomic DNA was extracted using the Qiagen DNeasy Blood and Tissue kit (Qiagen, # 69506) and quantified on a NanoDrop 1000. 5ug of DNA was digested to fragment size 200-300 base pairs using dsDNA Fragmentase enzyme at 370C for 30 minutes (New England Biolabs, #MO348L). Fragments were end-repaired, 3'-ends were adenylated, and appropriate adapter indexes were ligated using the Truseq protocol (Illumina). Between each reaction, fragments were cleaned using Agencourt AmPure magnetic beads (Beckman Coulter, # A63881). Fragments were then amplified by PCR at 980C/30 sec; 10 cycles of 980C/10sec, 600C/30sec, 720C/30sec; and 720C 5min with a hold at 100C. Enriched fragments were then cleaned using Agencourt AmPure magnetic beads and quantified before methylation-dependent immunoprecipitation. Methylated-DNA immunoprecipitation was performed using the Methylated-DNA IP kit
GEO Accession
GSM3401046