GSM3038964: Bisul-Col-0; Arabidopsis thaliana; Bisulfite-Seq
Total DNA from 10-day-old seedlings were extracted by CTAB, RNase treated and purified with phenol:chloroform followed by Ethanol precipitation. Approximately 1 ug of genomic DNA were used to generate libraries using premethylated adapters (NEXTFlex Bisulfite-Seq Adapters, Bioo Scientific #511911).The adaptor-ligated fragments were purified by QIAQuick column (QIAGEN) and bisulfite converted using the EpiTect Kit (Qiagen) following the manufacturer's instructions. The converted DNA was later enriched by PCR using the Pfu Turbo Cx Polymerase (Agilent), using the specific primers provided by Bios Scientific for enrichment.
GEO Accession
GSM3038964