GSM2948095: Oral; mouse metagenome; OTHER
Extraction of bacterial DNA was performed according to the manufacturer's instructions (NucleoSpin® Tissue Kit (Takara)). The V4 region of 16S rRNA genes was PCR amplified (25 cycles, primer pair F515/R806), followed by barcoding (8 cycles) using a Nextera XT Index Kit v2 (Illumina) in triplicate. Barcoded amplicons were sequenced (150-bp paired-end sequencing) on an Illumina MiSeq (Illumina).
GEO Accession
GSM2948095