Illumina HiSeq 3000 paired end sequencing; Illumina HiSeq 3000 paired end sequencing; Pig histone modification atlas
ChIP-Seq 100 cycle paired end experiment on an Illumina HiSeq 3000
NEBNext Ultra II DNA Library prep kit for Illumina
assay type
ChIP-seq
sample storage
frozen, -70 freezer
sample storage processing
cryopreservation, other
sampling to preparation interval
1
experimental protocol
https://www.neb.com/-/media/catalog/datacards-or-manuals/manuale7645.pdf
extraction protocol
http://ftp.faang.ebi.ac.uk/ftp/protocols/assays/ISU_SOP_ChIP_swine_immune_cells_20190124.pdf
library preparation location
DNA facility, Iowa State University
library preparation location longitude
not provided
library preparation location latitude
not provided
library preparation date
not provided
sequencing location
DNA facility, Iowa State University
sequencing location longitude
not provided
sequencing location latitude
not provided
sequencing date
not provided
experiment target
histone_modification
control experiment
ERX3212577
target protein name
Histone
chip protocol
http://ftp.faang.ebi.ac.uk/ftp/protocols/assays/ISU_SOP_ChIP_swine_immune_cells_20190124.pdf
chip antibody provider
Diagenode
chip antibody catalog
H3K4me3 (Diagenode, catalog: C15410003)
chip antibody lot
A1052D
library generation max fragment size range
600
library generation min fragment size range
50