RNA-seq of homo sapiens: human aortic endothelial cells
). cDNA libraries were generated by using KAPA Stranded mRNA-Seq Kit and KAPA Stranded RNA-Seq Kit with RiboErase (HMR) (Kapa Biosystems, Inc). Amplified cDNA was validated and quantified on Agilent Bioanalyzer with the High Sensitivity DNA chip. The purified libraries were normalized, pooled together, denatured, and diluted at final concentration of 1.8pM. Furthermore, 1.3mL of diluted pool was used to perform cluster generation, followed by 1 x 75 bp sequencing on NextSeq500 (Illumina).