GSM1446110: MD total RNA; Drosophila melanogaster; ncRNA-Seq
ovaries dissected, for IP samples: immunoprecipitation of Argonaute protein, extraction of small RNA, cloning of library. For total RNA libraries: isolation of total RNA after ovary dissection and cloning of small RNA library custom made protocol for cloning of piRNAs: isolation of small RNAs on acrylamide Gel or through IP of Argonaute, followed by 3' ligration, 5' ligation, Reverse transcription and final PCR amplification
GEO Accession
GSM1446110