GSM4518541: DU16-02S0003164_S12; Homo sapiens; RNA-Seq
Total RNA was extracted from human blood using the Qiagen PAXgene Blood RNA Kit according to the manufacturer's protocol. In the first batch, stranded mRNA sequencing libraries were prepared using the GLOBINclear Human Kit (Invitrogen) with a TruSeq Stranded mRNA Kit library Prep. In the second batch, stranded RNA-seq libraries were prepared using the commercially available Nugen Universal Plus mRNA-seq kit. Nugen's AnyDeplete mediated transcript depletion was used to eliminate globin mRNA transcripts from the final libraries. In the first batch libraries were sequenced at 50 bp paired-end on an Illumina HiSeq instrument. In the second batch libraries were sequenced at 50 bp paired-end on an Illumina NovaSeq instrument.
GEO Accession
GSM4518541