GSM3038261: Latency_model_SAHA_cell_95; Homo sapiens; RNA-Seq
Cells were washed and collected for bulk or single-cell RNA-Seq. On one hand, cells (1 mio) were resuspended in 400 μl lysis buffer of the ZR-Duet DNA/RNA miniprep (Zymo Research #D7001) and processed for RNA extraction according to manufacturer's instructions, and used for bulk RNA-Seq (Illumina HiSeq Ribo-Zero TruSeq str RNA-Seq). On the other hand, cells were resuspended at 0.5-1x106 cells/ml in R-10 (0.45 μm filtered) and processed for single cell isolation and cDNA synthesis on a small 5-10 μm fluidigm plate (fluidigm C1 Single Cell AutoPrep System, Clontech SMARTer Ultra Low RNA kit for Illumina sequencing), followed by single-cell RNA-Seq (Illumina Nextera XT DNA Sample Preparation). High-throughput sequencing (100 cycles, single end) was performed on Illumina HiSeq2500 (University of Lausanne, Genomic Technology Facility). Illumina HiSeq2500: 100 NT, single end (bulk and scRNA-Seq)
GEO Accession
GSM3038261