NextSeq 500 sequencing; 3' mRNA-seq of neurites and soma isolated from mESC-derived neurons
3' mRNA-seq of neurites and soma isolated from mESC-derived neurons
Mouse embryonic stem cells with doxycycline-inducible ASCL1 cassette (ASCL1-mESC) were differentiated into neurons and separated on neurites and soma as previously described (Zappulo et al.RNA localisation is a key determinant of neurite-enriched proteome. Nature Comm. 2017). RNA was extracted with TRIFast reagent (Peqlab). 3' mRNA-seq was performed with QuantSeq 3' mRNA-Seq kit (Lexogen 015) according to the manufacturer's recommendations.
Experimental Factor: fraction
soma