GSM1159895: siCTL_minusDHT_hs26l2_1; Homo sapiens; RNA-Seq
Nuclei were isolated from LNCaP cells with appropriate transfection and -/+ DHT treatment. GRO-seq libraries were prepared from 5 million cells based on previously published protocols (Wang et al., 2011). Libraries were sequenced on Illumina Hi-Seq 2000. After harvesting the cells, RNA polymerases were allowed to run-on for 5 minutes at 30C (~100bp) in the presence of sarkosyl and BrUTP. The RNA was then base hydrolyzed and purified. BrUTP-labeled run-on RNA was immunopurified with anti-BrdUTP-coated agar
GEO Accession
GSM1159895