GSM4454713: NH564; Mus musculus; RNA-Seq
RNA was extracted in TRIzol-Chloroform and purified using the RNeasy micro kit. RNA quality and quantity were assessed using RNA Screen Tape 6000 Pico LabChips with the Tape Station (Agilent Technologies). All RINs were higher than 9.0 The library was established using the Nugen Universal Plus mRNA-Seq kit, using 20 ng of total RNAs as recommended by the manufacturer. The oriented cDNAs produced from the poly-A+ fraction were PCR amplified (15-18 cycles). An equimolar pool of the final indexed RNA-Seq libraries was sequenced on an Illumina HiSeq2500, with paired-end reads of 130 bases and a mean sequencing depth of 58 millions per sample
GEO Accession
GSM4454713