unspecified sequencing; qiime_experiment_2192:1207288:449
study of families in their homes including microbial sampling of surfaces and human samapling (skin, gut).
This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to ??1,500 samples per lane, and both PCR primers contain sequencer adapter regions.
experiment_title
Gilbert_house_forensics_BE
target_subfragment
V4
sample_center
ANL
sequencing_meth
sequencing by synthesis
experiment_center
ANL
pcr_primers
FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
run_center
ANL
num_sequences
68951
platform
Illumina
library_construction_protocol
This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to ??1,500 samples per lane, and both PCR primers contain sequencer adapter regions.
experiment_design_description
study of families in their homes including microbial sampling of surfaces and human samapling (skin, gut).
row_number
449
target_gene
16S rRNA
study_center
ANL