GSM3402449: Chondrocytess from RCS cells ko for the FGF receptors FGFR3 and FGFR4, treated with FGF18, biological rep2; Rattus norvegicus; RNA-Seq
Extraction of total RNA was performed according to the manufacturer's instructions (RNeasy Mini Kit Cat No./ID: 74106 (250), Quiagen). RNA extract was precisely quantified and mixed at 50ng/5 µL Total RNA (100 ng) from each sample was prepared using QuantSeq 3'mRNA-Seq Library prep kit  (Lexogen, Vienna, Austria) according to manufacturer's instructions The amplified fragmented cDNA of 300 bp in size were sequenced in single-end mode using the NextSeq500 (Illumina) with a read length of 75 bp
GEO Accession
GSM3402449