Illumina WGS of Cafeteria roenbergensis: strain RCC970-E3
Genomic DNA was isolated using a Qiagen Blood & Cell Culture DNA Midi Kit from cells of clonal C. roenbergensis strains. Libraries were constructed with NEBNext High-Fidelity 2 PCR Master Mix chemistry and a reduced number of enrichment PCR cycles (six) to reduce AT-bias. Paired-end reads (2x300bp)  were sequenced on an Illumina MiSeq platform using MiSeq reagent kit version 3.