GSM2599106: col0_fb2.mRNAseq; Arabidopsis thaliana; RNA-Seq
TRIzol reagent (Thermo Fisher Scientific) For Samples 1 - 6, sRNA spike-in mix was added to total RNA followed by size-selection for 18-75 nt RNAs. The NEBnext small RNA library prep kit for Illumina (NEB) was then used to construct sRNA-Seq libraries. For Samples 7 - 12, ERCC spike-in mixes (LifeTech) were added to total RNA and 10 ng of total RNA/ERCC mix was used to generate mRNA-Seq libraries as described by Picelli et al. (2013) Nature Methods. All samples were sequenced on an Illumina Hi-Seq 2500 sequencing machine in either single read 50 base (sRNA-Seq) or paired-end read 50 base (mRNA-Seq) modes.
GEO Accession
GSM2599106