Acetonitrile preculture after subsequent acetic acid enrichment
100 g soil sample of the Experimental Botanical Garden Goettingen (Germany) was solved in 500 ml ddH2O and subsequently filtered with a 2.7 micrometer filter. 5 ml were added to 45 ml M9 with acetonitrile as sole carbon and nitrogen source and enriched for 160 h. The preculture was used to inoculate M9 with acetic acid and ammonia as sole carbon and nitrogen source (in triplicate). After reaching an OD600 of approx. 0.4, the cultures were harvested. DNA was isolated using the MasterPure complete DNA and RNA purification kit (Epicentre) and subsequently pooled. The sequencing library was generated with the Nextera XT DNA library prep kit (Illumina).