GSM3109291: PfOO-1; Plasmodium falciparum 3D7; RNA-Seq
Cells were lysed and RNA purified using a Qiagen Rneasy Kit with additional DNaseI on-column digestions. A barcoded library was made from each sample by using the Illumina TruSeq Stranded mRNA Library Prep Kit (Illumina, Cat# RS-122-2101) according to the manufacturer's protocol. qPCR was performed to determine the concentration of each library and an equimolar pool was made of all libraries. The library pool was sequenced on an Illumina HiSeq 2500 in Rapid Run mode according to the manufacturer's protocol. 100 nt single read RNA-sequencing
GEO Accession
GSM3109291