GSM2947784: Dnmt1 KO Jejunum; Mus musculus; Bisulfite-Seq
AllPrep DNA Spin Column Methyl-MidiSeq® libraries were prepared from 300 ng of genomic DNA digested with 40 units of BfaI, 40 units of MseI, and 80 units of MspI and the fragments produced were ligated to pre-annealed adapters containing 5'-methyl-cytosine instead of cytosine. Adapter-ligated fragments were filled in and 3'-terminal-A extended, then purified using the Zymo Research (ZR) DNA Clean & ConcentratorTM – 5 kit (Cat#: D4003). Bisulfite treatment of the fragments was done using the EZ DNA Methylation – Lightning kit (ZR, Cat#: D5030). PCR was performed and the size and concentration of the fragments were confirmed on the Agilent 2200 TapeStation, then sequenced on the Illumina Hiseq with 50 bp PE parameters.
GEO Accession
GSM2947784