GSM1446138: KPC tumour, GS_treated3; Mus musculus; RNA-Seq
Whole tumours were frozen in RNAlater and RNA was extracted using Qiagen RNeasy mini kit. The RNA library was prepared using TruSeq Stranded Total RNA library preparation with Ribozero Gold and quality measured on Agilent BioAnalyzer. The NGS was performed on HiSeq 2000 TM using V3 flow cells on one lane at 100bp paired-end. whole RNA was extracted from pellets of sorted myeloid cells using Qiagen RNeasy mini kit and stored in -80 until analysis. Libraries were made by using either IlluminaTrue Seq kits or Nugen Library Construction kits. Samples were multiplexed in 4 lanes and sequenced via the Illumina HiSeq2000 platform. 100 base-pair, paired-end data sequence data were then generated and subsequently analysed. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM1446138