GSM1444236: 16LV; Homo sapiens; OTHER
Genomic DNA was extracted from tumors after proteinase K digestion (Qiagen) overnight, using phenol-chloroform/ethanol precipitation. LAM-PCR was used for lentiviral and transposon genomic flank analysis. Nested PCR reactions were performed using primers Illumina-1 and Illumina-2. The PCR products were purified using a ChargeSwitch-Pro PCR Clean-up Kit, and all samples were combined at a 1:1 mass ratio. Internal vector products were further suppressed by implementing a second NarI/BamHI digest on the library, followed by amplification using a small quantity of the digest reaction as a template and the primers Illumina-1 and Illumina-2. The product from reaction was subsequently purified and submitted for next generation sequencing.
GEO Accession
GSM1444236