For chromatin immunoprecipitation-sequencing (ChIP-seq), 100?300 bp sized genomic libraries were generated with the input and the polymerase III-immunoprecipitated chromatin fragments using the Illumina TruSeq ChIP Sample Prep Kit. In briefly, the input and immunoprecipitated DNA fragments were ligated to one pair of adaptors for sequencing, size-fractioned to obtain 200- to 300-bp fragments on a 2% agarose gel, and subjected to 18 cycles of PCR. Each library was diluted to 8 pM for 76 cycles of single-read sequencing on the Illumina NextSeq 500 following the manufacturerĄŻs recommended protocol.