GSM2358571: 28 WT; Mus musculus; RNA-Seq
spleen was taken from sacrificed mice and smashed to obtain a cell suspensions. B cells were isolated using magnetic separation and total RNA was prepared using QIAGEN RNeasy kit (#74106) Total RNA samples were processed according to Dynabeads® mRNA DIRECTTM Purification Kit (Ambion) to isolate mRNA. The template library was prepared using the Ion Total RNA-Seq Kit v2 (Life Technologies). Quantity and size distribution of the library were analyzed using the Agilent Bioanalyzer 2100 DNA HS chip. Emulsion PCR using 10microl of 100pM library was performed using an OneTouch 2 instrument (Life Technologies) with an Ion PI Template OT2 200 kit following the manufacturer’s instructions. The enrichment of template library was achieved using the Ion OneTouch ES enrichment system (Life Technologies). Ion Proton sequencer and IPv2 chip were prepared according to the manufacturer’s recommendations.
GEO Accession
GSM2358571