Cell-free DNA sequencing of a Papio anubis: CWAF
Plasma was extracted from whole-blood samples from the non-human primates and stored at -80C. Samples were later thawed and cell-free DNA was extracted using the QIAmp Circulating Nucleic Acid Kit (Qiagen). Libraries were prepared using an automated microfluidics based platform (Mondrian ST; Ovation SP Ultralow Library Systems). Libraries were quantified on an Agilent 2100 Bioanalyzer (High Sensitivity DNA Kit) and sequenced on an Illumina NextSeq 500, in 2x75bp high output mode (~45 million reads/sample, 8 samples/library).