GSM3640263: RS006; Homo sapiens; RNA-Seq
The mononuclear cell (MNC) fraction from BM and/or PB was obtained by density gradient centrifugation using lymphoprep (PAA, Cölbe, Germany) according to standard procedures. For separation of different cell fractions, following thawing of viably frozen MNCs, cells were washed and stained with a panel of antibodies and sorted for purification of different cell fractions. Blast fraction was defined as CD34 positive. Sorting purity was defined as ≥95% and confirmed by reanalysis. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM3640263