GSM4089062: canine mammary tumour tissue's adjacent normal tissue [B2]; Canis lupus familiaris; RNA-Seq
Canine mammary tumour tissues and their adjacent normal tissues were immediately flash frozen with liquid nitrogen and the total RNA was extracted from the samples using Trizol reagent (TaKaRa, Japan), following the manufacturer's instructions. Total RNA was used for removing the rRNAs using Ribo-Zero rRNA Removal Kits (Illumina, USA) following the manufacturer's instructions. RNA libraries were constructed by using rRNA-depleted RNAs with TruSeq Stranded Total RNA Library Prep Kit (Illumina, USA) according to the manufacturer's instructions. Libraries were controlled for quality and quantified using  the BioAnalyzer 2100 system (Agilent Technologies, USA). 10 pM libraries were denatured as single-stranded DNA molecules, captured on Illumina flow cells, amplified in situ as clusters and finally sequenced for 150 cycles on Illumina HiSeq Sequencer according to the manufacturer's instructions.
GEO Accession
GSM4089062