RNA-Seq of VBNC cells
The mRNA enriched by removing ribosomal RNA was fragmented into short fragments using fragmentation buffer, and then the mRNA was reverse transcribed to cDNA with random primers. Second-strand cDNA were synthesized by Superscript Double-Stranded cDNA Synthesis kit (Invitrogen Inc., USA). The cDNA was purified by agarose gel electrophoresis and then amplified with PCR, and the sequence library was constructed.