Miseq of Teleogryllus oceanicus gut bacteria: lab female fed artificial diet sample 4
16S rRNA amplicons were generated in a single amplification step using  primers 515F (5- GTGCCAGCMGCCGCGGTAA -3) and R806 (5- GGACTACHVGGGTWTCTAAT -3). The amplicon library was prepared by pooling PCR products into equimolar ratios based on qPCR and quantification using a Labchip GX Touch HT (Perkin Elmer). Amplicons in the library were size-selected using a Pippin Prep (Sage Science) and purified using the Qiaquick PCR Purification Kit (Qiagen). The volume of purified library added for sequencing was determined using a Labchip GX Touch HT (Perkin Elmer), and sequenced (uni-directionally) using a 300 cycle MiSeq v2 Reagent Kit and standard flow cell on an Illumina MiSeq platform.