GSM3038520: MNP1; Mus musculus; miRNA-Seq
Nephron progenitors were isolated from intact whole embryonic kidney samples through limited digestion, followed by magnetic-activated cell sorting negative depletion of CD140a, CD105, Epcam and Ter119 in accordance to a published protocol (PMID: 26190145). Total RNA was extracted and purified using the Qiagen miRNeasy Micro Kit. Libraries were barcoded and prepared using the NEBNext Multiplex Small RNA Library Prep Kit according to the manufacturer's protocol.
GEO Accession
GSM3038520