Charles River C57BL/6N Mouse Cecum Metatranscriptome 5
Whole cecum was removed, flash-frozen in liquid nitrogen, and stored at -80 degrees C. Total RNA was extracted using the MOBIO PowerMicrobiome RNA extraction kit. Concentration and quality were evaluated using the NanoDrop ND-1000. DNA contamination was evaluated using a 16s rRNA PCR amplification and removed with the MOBIO RTS DNase kit. Samples were sent to the HudsonAlpha Genomic Services Laboratory for cDNA synthesis and paired-end sequencing on the illumina HiSeq 2000.