RNA-seq of Cercospora sojina: Wild-type in vitro
Tissue was collected, frozen in liquid nitrogen, and ground in a pre-chilled mortar and pestle containing sterile glass beads (0.5mm) (Research Products International). Total RNA was isolated with a Direct-zol RNA MiniPrep Kit (Zymo Research, Irvine, CA, USA) following the manufacturers recommendations. DNA quality and quantity were determined by agarose gel electrophoresis and a NanoDrop ND-1000 Spectrophotometer (Thermo Fisher Scientific). The library was sequenced with a 316 Chip Kit v2 (Thermo Fisher Scientific)