RNA-seq of Lialis burtonis: adult female blood
Illumina sequencing libraries were constructed by GeneCore (EMBL, Heidelberg, Germany). The barcoded stranded mRNA-sequencing libraries were prepared using the Illumina TruSeq mRNA v2 sample preparation kit (Illumina, San Diego, CA) with poly-A mRNA enrichment, implemented on the liquid handling robot Beckman FXP2. The Illumina libraries were pooled together and loaded on the Illumina NextSeq 500 sequencer and 84 bp were sequenced bi-directionally.