Transconjugant TCS-0.02 ppb
The sample was sequenced following the 1D genomic DNA selecting for long reads using SQKLSK108 protocol, which was similar to the 2D protocol, but with the use of Ampure XP beads instead of MyOne Streptavidin C1 beads after the ligation of the adaptors. The protocols were followed according to the manufacturers instructions except for the following steps: incubation time of 20 minutes in each endrepair and ligation steps, 0.7x AMPure XP beads for purification, and warm up elution buffer at 50C.