GSM4145697: ZAP-L 98F CLIP Reads Mapped to HIV-1 CG High; Homo sapiens; OTHER
UV-crosslinked cells were lysed, ZAP-RNA complexes were immunoprecipitatd and RNA was extracted for library construction. Libraries were prepared with sequential ligation of the following primers: 3' adapter: 5'adenylated/TCG TAT GCC GTC TTC TGC TTG-3'dideoxyC / 5' adapters containing the barcodes are as follows: rGUU CAG AGU UCU ACA GUC CGA CGA UC AGUGC rGUU CAG AGU UCU ACA GUC CGA CGA UC GAUGC rGUU CAG AGU UCU ACA GUC CGA CGA UC GUGGC rGUU CAG AGU UCU ACA GUC CGA CGA UC ACGGC rGUU CAG AGU UCU ACA GUC CGA CGA UC UAGGC rGUU CAG AGU UCU ACA GUC CGA CGA UC AUCGC Once the extracted RNA had the appropriate adaptors ligated, a gene specific primer was used to create and cDNA library. The DNA library was further amplified by PCR and purified on a TBE-Urea gel.
GEO Accession
GSM4145697