GSM3261516: TLE3 ChIP-seq, BAT adipocytes; Mus musculus; ChIP-Seq
For preparation of material for TLE3 ChIP-seq, eWAT-, iWAT-, and BAT-derived adipocytes were treated with 2 mM disuccinimidyl glutarate (DSG) (Proteochem) for 15 min followed by cross-linking with 1% formaldehyde for 30 min. Crosslinking was stopped by addition of glycine. Nuclei isolation, chromatin preparation and chromatin IP was performed as described previously (Siersbæk et al., 2012, MCB, 32: 3452-3463)). ChIP-seq libraries were constructed according to the manufacturer's instructions (Illumina) as described in (Nielsen R, Mandrup S, 2014 Genome-Wide Profiling of Transcription Factor Binding and Epigenetic Marks in Adipocytes by ChIP-seq. Methods in Enzymology 2014, Vol. 537, pp. 261-279).
GEO Accession
GSM3261516