16S amplicon seq of S. briareus stomach and gut microbiome
Amplification of the V4 region of the 16S rRNA gene was performed using the Earth Microbiome Project universal primers 515f–806r. In this study, there are 191 samples. To demultiplex, use the barcodes.fasta file, which associates each sequence with a barcode corresponding to one of the 191 samples. Demultiplex the samples using the corresponding barcodes are as follows (sample_barcode, host, tissue, location, experiment day): AATTTAGGTAGG, Gracilaria, Gracilaria, Hamblin Pond, Day_0; GACTCAACCAGT, Gracilaria, Gracilaria, Hamblin Pond, Day_0; GCCTCTACGTCG, Gracilaria, Gracilaria, Hamblin Pond, Day_0; TGTCAAAGTGAC, Sclerodactyla briareus, Intestine, Jehu Pond, Day_0; ACTCCCGTGTGA, Sclerodactyla briareus, Intestine, Jehu Pond, Day_0; GACTCTGCTCAG, Sclerodactyla briareus, Intestine, Jehu Pond, Day_0; TACCGAAGGTAT, Sclerodactyla briareus, Intestine, Jehu Pond, Day_0; AATGGTTCAGCA, Sclerodactyla briareus, Intestine, Jehu Pond, Day_0; CGCACCCATACA, Sclerodactyla briareus, Intestine, Jehu Pond, Day_0; ACTCTTACTTAG, Sclerodactyla briareus, Intestine, Jehu Pond, Day_0; CAAGTGAAGGGA, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_0; TAATCGGTGCCA, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_0; CGGGACACCCGA, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_0; TATGCCAGAGAT, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_0; AAGAACTCATGA, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_0; CGGTGACCTACT, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_0; CTTGATTCTTGA, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_0; GAGATACAGTTC, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_0; ACCTTACACCTT, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_0; TTGGAACGGCTT, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_0; TACGGATTATGG, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_0; CAGTAGCGATAT, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_0; AGGATCAGGGAA, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_0; TATTGCAGCAGC, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_0; GTAGTAGACCAT, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_0; CTCGCCCTCGCC, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_0; ACATACTGAGCA, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_0; CCACTCTCTCTA, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_0; ACTTGGTGTAAG, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_0; CACGCAGTCTAC, Sclerodactyla briareus, Intestine, Jehu Pond, Day_0; CCGGACAAGAAG, Sclerodactyla briareus, Intestine, Jehu Pond, Day_0; TGGACTCAGCTA, Sclerodactyla briareus, Intestine, Jehu Pond, Day_0; CAAACGCACTAA, Sclerodactyla briareus, Intestine, Jehu Pond, Day_0; TGAGTGGTCTGT, Sclerodactyla briareus, Intestine, Jehu Pond, Day_0; ACGACGCATTTG, Sclerodactyla briareus, Intestine, Jehu Pond, Day_0; AGAGCCAAGAGC, Sclerodactyla briareus, Intestine, Jehu Pond, Day_0; TAAATATACCCT, Sclerodactyla briareus, Intestine, Jehu Pond, Day_0; GTCGTCCAAATG, Sclerodactyla briareus, Intestine, Jehu Pond, Day_0; TTACTGTGGCCG, Sclerodactyla briareus, Intestine, Jehu Pond, Day_0; TAACAATAATTC, Sclerodactyla briareus, Intestine, Jehu Pond, Day_0; GCTCGAAGATTC, Sclerodactyla briareus, Intestine, Jehu Pond, Day_0; ACTACTGAGGAT, Seawater_pond, Seawater_pond, Jehu Pond, Day_0; AATTCACCTCCT, Seawater_pond, Seawater_pond, Jehu Pond, Day_0; CGTATAAATGCG, Seawater_pond, Seawater_pond, Jehu Pond, Day_0; AGCCTTCGTCGC, Seawater_pond, Seawater_pond, Hamblin Pond, Day_0; TCCATACCGGAA, Seawater_pond, Seawater_pond, Hamblin Pond, Day_0; AGCCCTGCTACA, Seawater_pond, Seawater_pond, Hamblin Pond, Day_0; ATGCTGCAACAC, Sediment_pond, Sediment_pond, Jehu Pond, Day_0; ACTCGCTCGCTG, Sediment_pond, Sediment_pond, Jehu Pond, Day_0; TTCCTTAGTAGT, Sediment_pond, Sediment_pond, Jehu Pond, Day_0; CGTCCGTATGAA, Sediment_pond, Sediment_pond, Jehu Pond, Day_0; CCTAACGGTCCA, Sediment_pond, Sediment_pond, Hamblin Pond, Day_0; CGCGCCTTAAAC, Sediment_pond, Sediment_pond, Hamblin Pond, Day_0; TATGGTACCCAG, Sediment_pond, Sediment_pond, Hamblin Pond, Day_0; TACAATATCTGT, Sediment_pond, Sediment_pond, Hamblin Pond, Day_0; TATTTGATTGGT, Sclerodactyla briareus, Stomach, Jehu Pond, Day_0; CTATGTATTAGT, Sclerodactyla briareus, Stomach, Jehu Pond, Day_0; CGGTATAGCAAT, Sclerodactyla briareus, Stomach, Jehu Pond, Day_0; TAGGAGAGACAG, Sclerodactyla briareus, Stomach, Jehu Pond, Day_0; TGAGTATGAGTA, Sclerodactyla briareus, Stomach, Jehu Pond, Day_0; GAACCAGTACTC, Sclerodactyla briareus, Stomach, Jehu Pond, Day_0; GTGCCATAATCG, Sclerodactyla briareus, Stomach, Jehu Pond, Day_0; GCCTATGAGATC, Sclerodactyla briareus, Stomach, Hamblin Pond, Day_0; CACGTTTATTCC, Sclerodactyla briareus, Stomach, Hamblin Pond, Day_0; TGACTAATGGCC, Sclerodactyla briareus, Stomach, Hamblin Pond, Day_0; CTGTCTATACTA, Sclerodactyla briareus, Stomach, Hamblin Pond, Day_0; CGTTTGGAATGA, Sclerodactyla briareus, Stomach, Hamblin Pond, Day_0; TGATATCGTCTT, Sclerodactyla briareus, Stomach, Hamblin Pond, Day_0; AATGCGCGTATA, Sclerodactyla briareus, Stomach, Hamblin Pond, Day_0; GAAATCTTGAAG, Sclerodactyla briareus, Stomach, Hamblin Pond, Day_0; GTGGAGTCTCAT, Sclerodactyla briareus, Stomach, Hamblin Pond, Day_0; TAATCTCGCCGG, Sclerodactyla briareus, Stomach, Hamblin Pond, Day_0; ACGCTTAACGAC, Sclerodactyla briareus, Stomach, Hamblin Pond, Day_0; ATACATGCAAGA, Sclerodactyla briareus, Stomach, Hamblin Pond, Day_0; AATCTTGCGCCG, Sclerodactyla briareus, Stomach, Hamblin Pond, Day_0; AATAACTAGGGT, Sclerodactyla briareus, Stomach, Hamblin Pond, Day_0; TGATGTGCTAAG, Sclerodactyla briareus, Stomach, Hamblin Pond, Day_0; AGTAAAGATCGT, Sclerodactyla briareus, Stomach, Hamblin Pond, Day_0; GCGATGGCGATG, Sclerodactyla briareus, Stomach, Hamblin Pond, Day_0; GTTGATACGATG, Sclerodactyla briareus, Stomach, Hamblin Pond, Day_0; TGAGACCCTACA, Sclerodactyla briareus, Stomach, Hamblin Pond, Day_0; ATTACGTATCAT, Sclerodactyla briareus, Stomach, Jehu Pond, Day_0; TGTGCACGCCAT, Sclerodactyla briareus, Stomach, Jehu Pond, Day_0; TTGCTGGACGCT, Sclerodactyla briareus, Stomach, Jehu Pond, Day_0; GCGATCACACCT, Sclerodactyla briareus, Stomach, Jehu Pond, Day_0; GAAGAGGGTTGA, Sclerodactyla briareus, Stomach, Jehu Pond, Day_0; TTACACAAAGGC, Sclerodactyla briareus, Stomach, Jehu Pond, Day_0; TATCCAAGCGCA, Sclerodactyla briareus, Stomach, Jehu Pond, Day_0; GGTGAGCAAGCA, Sclerodactyla briareus, Stomach, Jehu Pond, Day_0; TTGCGGACCCTA, Sclerodactyla briareus, Stomach, Jehu Pond, Day_0; TGCACAGTCGCT, Sclerodactyla briareus, Stomach, Jehu Pond, Day_0; GGTTCATGAACA, Sclerodactyla briareus, Stomach, Jehu Pond, Day_0; CTTATTAAACGT, Sclerodactyla briareus, Stomach, Jehu Pond, Day_0; ACGTGAGGAACG, Zostera_marina, Zostera_marina, Jehu Pond, Day_0; GGTTGCCCTGTA, Zostera_marina, Zostera_marina, Jehu Pond, Day_0; CATATAGCCCGA, Zostera_marina, Zostera_marina, Jehu Pond, Day_0; TAATCCATAATC, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_13; CCTCCGTCATGG, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_13; TTCGATGCCGCA, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_13; AGAGGGTGATCG, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_13; AGCTCTAGAAAC, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_13; CTGACACGAATA, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_13; GCTGCCCACCTA, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_13; GCGTTTGCTAGC, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_13; AGATCGTGCCTA, Sclerodactyla briareus, Intestine, Jehu Pond, Day_13; AATTAATATGTA, Sclerodactyla briareus, Intestine, Jehu Pond, Day_13; CATTTCGCACTT, Sclerodactyla briareus, Intestine, Jehu Pond, Day_13; CATGATTAAGAG, Sclerodactyla briareus, Intestine, Jehu Pond, Day_13; GCAACGAACGAG, Sclerodactyla briareus, Intestine, Jehu Pond, Day_13; GTGCCGGCCGAC, Seawater_tank, Seawater_tank, Tank, Day_13; CCTTGACCGATG, Seawater_tank, Seawater_tank, Tank, Day_13; CAAACTGCGTTG, Seawater_tank, Seawater_tank, Tank, Day_13; TACCTGTGTCTT, Sediment_tank, Sediment_tank, Tank, Day_13; CTTGGAGGCTTA, Sediment_tank, Sediment_tank, Tank, Day_13; TATCATATTACG, Sediment_tank, Sediment_tank, Tank, Day_13; TCGTTATTCAGT, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_17; AATGTTCAACTT, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_17; AGCAGTGCGGTG, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_17; CCTCACTAGCGA, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_17; CTACTCCACGAG, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_17; AGCGCGGCGAAT, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_17; TCGAGAGTTTGC, Seawater_tank, Seawater_tank, Tank, Day_17; CGACACGGAGAA, Seawater_tank, Seawater_tank, Tank, Day_17; TCCACAGGGTTC, Seawater_tank, Seawater_tank, Tank, Day_17; CTATATTATCCG, Sediment_inside_cuke, Sediment_inside_cuke, Tank, Day_17; ACCGAACAATCC, Sediment_inside_cuke, Sediment_inside_cuke, Tank, Day_17; ACGGTACCCTAC, Sediment_tank, Sediment_tank, Tank, Day_17; TGAGTCATTGAG, Sediment_tank, Sediment_tank, Tank, Day_17; ACCTACTTGTCT, Sediment_tank, Sediment_tank, Tank, Day_17; AGATGTCCGTCA, Sclerodactyla briareus, Stomach, Hamblin Pond, Day_17; GGATACTCGCAT, Sclerodactyla briareus, Stomach, Hamblin Pond, Day_17; GCATATGCACTG, Sclerodactyla briareus, Stomach, Hamblin Pond, Day_17; CCGGCGACAGAA, Sclerodactyla briareus, Stomach, Hamblin Pond, Day_17; CTAATCAGAGTG, Sclerodactyla briareus, Stomach, Hamblin Pond, Day_17; ACCTATGGTGAA, Sclerodactyla briareus, Stomach, Hamblin Pond, Day_17; AATCTAACAATT, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_20; CCACGAGCAGGC, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_20; CAAAGGAGCCCG, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_20; CGCTGTGGATTA, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_20; ACGACAACGGGC, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_20; TAGTCTAAGGGT, Sclerodactyla briareus, Intestine, Hamblin Pond, Day_20; GTTTGAAACACG, Sclerodactyla briareus, Intestine, Jehu Pond, Day_20; CCAACCCAGATC, Sclerodactyla briareus, Intestine, Jehu Pond, Day_20; AGCCGCGGGTCC, Sclerodactyla briareus, Intestine, Jehu Pond, Day_20; CGATGAATATCG, Sclerodactyla briareus, Intestine, Jehu Pond, Day_20; ATGACTAAGATG, Sclerodactyla briareus, Intestine, Jehu Pond, Day_20; TGACAGAATCCA, Sclerodactyla briareus, Intestine, Jehu Pond, Day_20; GGAGAACGACAC, Seawater_tank, Seawater_tank, Tank, Day_20; CCTACCATTGTT, Seawater_tank, Seawater_tank, Tank, Day_20; TCCGGCGGGCAA, Seawater_tank, Seawater_tank, Tank, Day_20; AACAGTAAACAA, Sediment_inside_cuke, Sediment_inside_cuke, Tank, Day_20; GTTCATTAAACT, Sediment_inside_cuke, Sediment_inside_cuke, Tank, Day_20; ACTGTGACGTCC, Sediment_tank, Sediment_tank, Tank, Day_20; CTCTGAGGTAAC, Sediment_tank, Sediment_tank, Tank, Day_20; CATGTCTTCCAT, Sediment_tank, Sediment_tank, Tank, Day_20; TCAGACCAACTG, Sclerodactyla briareus, Stomach, Hamblin Pond, Day_20; GCGTGCCCGGCC, Sclerodactyla briareus, Stomach, Hamblin Pond, Day_20; TGCGGCGTCAGG, Sclerodactyla briareus, Stomach, Hamblin Pond, Day_20; CTTGCTCATAAT, Sclerodactyla briareus, Stomach, Hamblin Pond, Day_20; CTAGCGTGCGTT, Sclerodactyla briareus, Stomach, Hamblin Pond, Day_20; ACCTCAGTCAAG, Sclerodactyla briareus, Stomach, Jehu Pond, Day_20; TCATTAGCGTGG, Sclerodactyla briareus, Stomach, Jehu Pond, Day_20; TAAACCTGGACA, Sclerodactyla briareus, Stomach, Jehu Pond, Day_20; TTAAGTTAAGTT, Sclerodactyla briareus, Stomach, Jehu Pond, Day_20; GGTAGTTCATAG, Sclerodactyla briareus, Stomach, Jehu Pond, Day_20; GTTCTAAGGTGA, Sclerodactyla briareus, Stomach, Jehu Pond, Day_20; TACAGCGCATAC, Sclerodactyla briareus, Stomach, Jehu Pond, Day_20; ACTCTGTAATTA, Sclerodactyla briareus, Intestine, Hamblin Pond, Tank_control; CAATCATAGGTG, Sclerodactyla briareus, Intestine, Hamblin Pond, Tank_control; GTCACTCCGAAC, Sclerodactyla briareus, Intestine, Hamblin Pond, Tank_control; TAGTTCGGTGAC, Sclerodactyla briareus, Intestine, Hamblin Pond, Tank_control; TCAAGTCCGCAC, Sclerodactyla briareus, Intestine, Hamblin Pond, Tank_control; GTCAGGTGCGGC, Sclerodactyla briareus, Intestine, Hamblin Pond, Tank_control; ATATGTTCTCAA, Sclerodactyla briareus, Intestine, Jehu Pond, Tank_control; CCGATAAAGGTT, Sclerodactyla briareus, Intestine, Jehu Pond, Tank_control; GCATAAACGACT, Sclerodactyla briareus, Intestine, Jehu Pond, Tank_control; ACTAAAGCAAAC, Sclerodactyla briareus, Intestine, Jehu Pond, Tank_control; GTCCGTCCTGGT, Sclerodactyla briareus, Intestine, Jehu Pond, Tank_control; TTCCAATACTCA, Sclerodactyla briareus, Intestine, Hamblin Pond, Tank_control; TAGCAGTTGCGT, Sclerodactyla briareus, Stomach, Hamblin Pond, Tank_control; TCATGGCCTCCG, Sclerodactyla briareus, Stomach, Hamblin Pond, Tank_control; GTTGGACGAAGG, Sclerodactyla briareus, Stomach, Hamblin Pond, Tank_control; CGTTCTGGTGGT, Sclerodactyla briareus, Stomach, Hamblin Pond, Tank_control; TTAATGGATCGG, Sclerodactyla briareus, Stomach, Hamblin Pond, Tank_control; CACACAAAGTCA, Sclerodactyla briareus, Stomach, Hamblin Pond, Tank_control; TTGAACAAGCCA, Sclerodactyla briareus, Stomach, Jehu Pond, Tank_control; ATGTGCTGCTCG, Sclerodactyla briareus, Stomach, Jehu Pond, Tank_control; CAGGAACCAGGA, Sclerodactyla briareus, Stomach, Jehu Pond, Tank_control; ATCGTAGTGGTC, Sclerodactyla briareus, Stomach, Jehu Pond, Tank_control; TAGGAACTCACC, Sclerodactyla briareus, Stomach, Jehu Pond, Tank_control; CGAGGCGAGTCA, Sclerodactyla briareus, Stomach, Hamblin Pond, Tank_control