RNA-Seq of Saccharomyces cerevisiae: Mid-log Phase cells
Fermentations on simulated grape juice were performed on 1-L asks with 800-mL volume in three parallel experiments. Samples were collected in mid-log phase of fermentation for RNA extraction. Total RNA was extracted based on Trizol reagent (Invitrogen, USA) . The agarose gel electrophoresis and Agilent 2100 were used to detect the purity and concentration of samples to satisfy the requirement of RNA-seq. The cDNA library construction and RNA sequencing were carried out at BGI (Shenzhen, China) utilizing standard protocols.