Full genome sequencing of exposed Escherichia coli
Libraries were prepared with TruSeq Nano HT (Illumina, San Diego, USA) and fragmented to an average size of 350-400 bp with CovarisE220. Average library size after adapter ligation was 500-550 bp, determined by Fragment Analyzer and Standard Sensitivity NGS kit. Concentrations of the final libraries were measured by Qubit, dsDNA broad range assay. Libraries were used for paired-end sequencing of genomes using the Illumina sequencing technology on a NextSeq v2 Mid Output 300 cycles (2x150).