GSM2438500: WT maltose #2; Neurospora crassa; RNA-Seq
Mycelia were harvested by filtration and flash frozen in liquid nitrogen. Total RNA was extracted by bead-beating in TRIZOL reagent and purified with RNeasy kit from Qiagen. Total RNA starting material was 1 µg per sample and 10 cycles of PCR was used for library amplification. The prepared libraries were then quantified using KAPA Biosystem’s next-generation sequencing library qPCR kit and run on a Roche LightCycler 480 real-time PCR instrument. The quantified libraries were then multiplexed into pools of 9 libraries, and the pool was then prepared for sequencing on the Illumina HiSeq2000 sequencer.
GEO Accession
GSM2438500