GSM4202339: 3'mRNA-seq of Satellite Stem Cells treated with lnc-023  GAPMERS rep4; Mus musculus; RNA-Seq
Total RNA from myoblasts/myotubes and SCs was extracted with TriReagent (Sigma). 0.5–1ug of RNA were treated with RNase-free DNase I enzyme (Thermo scientific). Libraries were prepared from 100 ng of total RNA using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen GmbH). Quality of libraries was assessed by using screen tape High sentisivity DNA D1000 (Agilent Technologies). 3'mRNA-seq
GEO Accession
GSM4202339