Fecal metagenome of a rabbit with gastroenteritis
Randomly primed cDNA was prepared in reverse transcription reactions containing 1x reaction buffer, 5 mM DTT, 1.25 mM dNTPs, 20 pmoles random hexamer primer, 100 U Superscript III (Invitrogen), and 100 ng RNA template. Second strand DNA was synthesized as above using Sequenase DNA polymerase. cDNA was adapted for Ion Torrent sequencing using the Ion Xpress Library Kit (catalog # 4468987) according to the manufacturer’s protocols.