Whole genome sequencing of Cedrela odorata
Fresh leaf tissue was collected from Cedrela odorata L. at NYBG (Table 1; NYBG Accession 683/89; Bronx, NY, USA; Oregon State University Herbarium voucher OSC-V-258305; referred to as CEOD-NYBG), and frozen on dry ice for total RNA and DNA extraction. To construct the DNA library, 500 ng of total genomic DNA was sheared to a modal size of 130 bp using sonication (30 cycles; 30 sec on + 30 sec off; Diagenode BioRuptor, Denville, NJ, USA), and adapted an Illumina genomic library using the NEBNext Ultra II Kit (New England Biolabs Inc., Ipswich, MA, USA). The mRNA and DNA libraries from CEOD-NYBG were uniquely indexed, pooled to equal mass representation, and sequenced using one lane of 100 bp paired-end sequencing on an Illumina HiSeq 3000 (Center for Genome Research and Biocomputing, Oregon State University, Corvallis, OR, USA).