Single neuron CNV analysis in mouse brain
MALBAC whole genome amplification was performed on a single FACS-sorted (NeuN+) neuronal nucleus as in Burbulis, et al. (PMID 30055482) Bioo library prep was used to add Illumina barcodes. Library fragments were electrophoresed and size-selected to 300-700 bp. Following gel extraction and purification, the library was pooled with other barcoded libraries for sequencing.