GSM4202301: HCI-005_AR_Veh; Homo sapiens; ChIP-Seq
Tumors were cut into 30µm slices on a cryostat and fixed with 2mM disuccinimidyl glutarate for 25m followed by 1%formaldehyde for 20m prior to quenching. Chromatin was prepared and subsequently sheared by the Diagenode BioRuptor Pico for 5.5 cycles (30 sec on, 30 sec off) on ice. Sheared chromatin was immunoprecipitated with 5µg AR, 5µg ER, or 2µg H3K27Ac antibody overnight, after coupling to Protein A Dynabeads (10002D). DNA was purified using phenol:chloroform:isoamyl alcohol extraction. Libraries were prepared according to manufacturer's instructions accompanying the Qiagen Ultralow Input Library Kit (Part# 180495). ChIP-Seq; Illumina NextSeq500 (High Output v2) single-end 75 bp reads.
GEO Accession
GSM4202301