GSM1440707: H3K27ac ChIP-Seq replicate 1 (0 hr before starvationr; baseline); Chlamydomonas reinhardtii; ChIP-Seq
ChIP-seq was performed as previously described (Chen et al., 2008). Briefly, cross-linked cells were lysed with Lysis Buffer (50 mM Tris-HCl pH8, 5 mM EDTA, 1% SDS) and chromatin was then sheared. 5-50 ug of chromatin was used in each immuno-precipitation.  Chromatin was first pre-cleared and then mixed with beads pre-bound with antibody. Beads were washed and eluted at 37ºC. The eluate was then de-crosslinked and DNA was purified by phenol/ chloroform extraction, followed by ethanol precipitation.   * Chen et al. 2008. Integration of external signaling pathways with the core transcriptional network in embryonic stem cells. Cell 133, 1106-1117. 5-10 ng of purified DNA was used in ChIP-seq library preparation using Truseq DNA Sample Preparation Kit (Illumina)
GEO Accession
GSM1440707