GSM4023954: sc-N2_32_pooling_after; Homo sapiens; RNA-Seq
Total RNA was extracted and processed following the SMART-seq protocol from Takada and NEXTERA from Illumina, following in both cases the Takada's protocol that allows full-length coverage of gene expression at the level of single cell. Samples were processed using the SMART-seq protocol from Takada and NEXTERA from Illumina, following in both cases the Takada's protocol that allows full-length coverage of gene expression at the level of single cell.RNA quality. DNA quality and size was examined for every individual cells using Bioanalyzer (Agilent Technologies). Only samples with detectable DNA and size in accord with not prior RNA degradation were selected for sequencing. Libraries were pooled before cleaning up and final DNA concentration measuring (these samples represent "pooling_before," or [a] files; while the same libraries were pooled after. cleaning and final DNA concentration measuring (these samples represent "pooling_after," or [b] files; in short, therefore, each single cell  library was sequenced twice,  in onec case the exact concentration of each library in the pool was not adjusted individually, while in the other case it was adjusted before pooling; these two options are suggested in the Takada's protocol and we aimed to test both).
GEO Accession
GSM4023954