GSM1446064: ChIP-seq, Liver_GR_GRdim_pred_6am; Mus musculus; ChIP-Seq
Liver tissue isolated from sacrificed mice was immediately frozen in liquid nitrogen. To process for ChIP, the tissue was thawed, minced and crosslinked in 1 % formaldehyde/PBS. Cells were disrupted and nuclei purified by dounce homogenization in a hypotonic buffer (20 mM HEPES/NaOH pH 7.5, 0.25 M sucrose, 3 mM MgCl2, 0.2% NP-40, 3 mM 2-mercaptoethanol, Complete protease inhibitor cocktail). To prepare ChIP extracts, nuclei were suspended SDS lysis buffer (50 mM HEPES/NaOH pH 7.5, 1% SDS, 10 mM EDTA, Complete protease inhibitor), incubated at 4 C for 10 min, and subjected to microtip probe sonication under conditions optimized for IP efficiency. ChIP-Seq libraries were produced and sequenced according to Illumina protocols. ChIP-exo libraries were produced for Illumina sequencing. Libraries were multiplexed for sequencing.
GEO Accession
GSM1446064