Subject 1 - LCL
1g genomic DNA was randomly fragmented by Covaris. Fragmented DNA was tested by gel electrophoresis and purified by AxyPrep Mag PCR clean up kit. Fragmented DNA was combined with End Repair Mix, incubated at 20 for 30 min, and end-repaired DNA was purified again using AxyPrep Mag PCR clean up kit. Repaired DNA was combined with A-Tailing Mix and incubated at 37 for 30 min. Illumina adapters were ligated to the adenylated 3' ends, and incubated at 16 for 16h. Adapter-ligated DNA was purified with AxyPrep Mag PCR clean up kit and fragments selected based on size. Just enough rounds of PCR amplification were used to obtain sufficient DNA using adapter primers and PCR master mix. Products were again purified with AxyPrep Mag PCR clean up kit. Library was validated by the Agilent Technologies 2100 bioanalyzer and ABI StepOnePlus RealTime PCR System and qualified libraries were pair-end sequenced using the HiSeq Xten Illumina System.