16S oyster May 17, 2018: Sequim, on-bottom, low density, replicate 7
DNA was extracted using the Qiagen DNeasy PowerSoil kit and used for an initial PCR amplification of the 16S rRNA gene (V3-4) with primers 341F/806R and Fluidigm CS1/CS2 linkers. A second PCR was run to include barcodes and sequencing adaptors (Fluidigm Access Array Barcode Library for Illuimina Sequencers). Final PCR products were pair-end sequenced (2 x 300 bp) on an Illumina MiSeq.