RNA-Seq of Limnoria tripunctata: whole animals
25 whole animals were collected in seawater on ice, liquid removed and flash frozen in liquid nitrogen before homogenization with a micropestle and total RNA extraction with the TRIzol Reagent (Thermo Fisher Scientific). Total RNA was treated with RQ1 DNase (Promega) to remove genomic DNA, cleaned with RNA Clean & Concentrator-5 (Zymo Research), then quantified spectrophotometrically (NanoDrop 2000, Thermo Scientific) and its integrity evaluated using the Bioanalyzer2100 (Agilent). The transcriptomic library was constructed after poly-A selection using the TruSeq Stranded mRNA Sample Preparation Kit (Illumina) according to the library protocol.