Illumina HiSeq 2500 paired end sequencing; RNA-seq comparison of fadR deletion mutant and the wild type strain
RNA-seq comparison of fadR deletion mutant and the wild type strain
cells were pelleted from WT and KO cultures in early exponential growth phase at an OD600 between 0.2 and 0.3.  Total RNA samples were extracted by miRNeasy Mini Kit (Qiagen) sequencing libraries were prepared with TruSeq Stranded mRNA Library Prep Kit (Illumina) according to the manufacturer's protocol (#15031047,rev E) with the exception that no polyA selection was performed.
Experimental Factor: genotype
fadR deletion mutant