GSM3577536: Nfe2 KO CD41pos cells from adult bone marrow; Mus musculus; RNA-Seq
A 10X Genomics Chromium machine was used for 3,900-6,900 single cell capture and cDNA was prepared using the Single Cell 3' Protocol recommended by the manufacturer. The 10X Genomics Chromium machine partitions cells into Gel Bead-In-EMulsions (GEMs), where all generated cDNA share a common 10x Genomics barcode but uses a pool of  about 750,000 barcodes to separately index each cell's transcriptome. The P7 and R2 primers were added during the GEM incubation and the P5, and R1 during library construction via end repair, A-tailing, adaptor ligation and PCR. The final libraries contained the P5 and P7 primers used in Illumina bridge amplification. The sequencing was carried out on an Illumina Nextseq 500 to achieve paired-end 75 bp reads.
GEO Accession
GSM3577536