GSM4089040: NHLF_siNC; Homo sapiens; RNA-Seq
Total RNA was extracted from NHLFs 48 h after siRNA transfection using the RNeasy Mini Kit (Qiagen). The mRNA from eukaryotic organisms was enriched using oligo(dT) beads. First, the mRNA was fragmented randomly by adding fragmentation buffer, then the cDNA was synthesized by using mRNA template and random hexamers primer, after which a custom second-strand synthesis buffer (Illumina) , dNTPs, RNase H and DNA polymerase I were added to initiate the second-strand synthesis. Second, after a series of terminal repair, A ligation and sequencing adaptor ligation, the double-stranded cDNA library was completed through size selection and PCR enrichment.
GEO Accession
GSM4089040