p65 ChIP-seq 10min TNF
Approximately 10 million HUVECs were cross-linked (10 min; 20°C) 10 after TNFα stimulation, chromatin was fragmented by sonication (Bioruptor; Diagenode), immunoprecipitated using a a rabbit polyclonal against the full-length p65 subunit of NF-κB (39369, Active motif) on aliquots of ~25 μg chromatin. Immunoprecipitated complexes were then washed and eluted using the ChIP-It-Express kit (Active motif).