Illumina HiSeq 2500 sequencing; Cold induces a β-adrenergic–independent thermogenic gene program in subcutaneous adipose tissue.
Cold induces a β-adrenergic–independent thermogenic gene program in subcutaneous adipose tissue.
inguinal white adipose tissue The mice were kept under 4C for 2.5 days. The cDNA libraries were amplified by single primer isothermal amplification using an Ultralow DR library kit (NuGEN) according to the manufacturer's instructions. The qualities of the libraries were determined by Bioanalyzer (Agilent Technologies).
Experimental Factor: wild type genotype
genotype
Experimental Factor: Cold (4 degree)
stimulus