GSM1982402: S42/M1LAC+.8; Mus musculus; OTHER
Enterocytes were isolated from every small intestine segment (approx. 100 mg) using a chelating method (Weiser MM (1973). J Biol Chem, 248(7):2536-41). DNA was extracted from enterocytes and the small intestinal segment lacking enterocytes using standard phenol-chloroform DNA extraction methods in combination with Phase-Lock tubes (5 Prime) after an overnight treatment in Proteinase K. DNA quantity was measured by NanoDrop ND-1000, and DNA quality was verified on a 1% agarose gel. DNA modification state was examined with single nucleotide resolution in the Lct and Mcm6 loci of mice using the bisulfite padlock probe technique (Diep D et al (2012). Nat Methods 9(3):270-2). 314 padlock probes with 6 base pairs of degenerate sequences were targeted to unique (non-repetitive) genomic sequences (GRCm38/mm10). Probes covered 90kb of the region of interest on for both forward and reverse DNA strands. Target DNA regions were designed for bisulfite converted DNA using ppDesigner software.
GEO Accession
GSM1982402