18S Illumina Miseq kotaomo
The library construction of the 16S rRNA gene was performed according to Caporaso et al. (2012), using universal prokaryotic primers 515F and 806R with Illumina adapter and Golay barcode sequences. and for 18S rRNA gene library construction was performed according to Caporaso et al. (2012)using universal eukaryotic primers Illumina_Euk_1391F and Illumina_EukBr with appended Illumina adapter and Golay barcode sequences, and mammal blocking primer.