GSM3107841: BI-2; Homo sapiens; RNA-Seq
RNA extraction from organoids from NC and CeD donors was carried out in TRIzol reagent (#15596018, ThermoFischer Scientific, MA) accordingly to manufacturer's directions and flash frozen at -80oC for further RNA purification. Total RNA was purified using Zymo-Spin column (#R2050, Zymo Research, Tustin, CA), accordingly to manufacturer's protocol. Total RNA was subjected to polyA selection, followed by library construction using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, MA)
GEO Accession
GSM3107841