GSM4090749: pifu-Y; Rattus norvegicus; RNA-Seq
The organs and tissues were isolated from young, old, and calorie-restricted (CR) SD rats. Pooled samples of 2 to 3 rats were quickly minced into ∼1 mm3 pieces on ice with scissors in cold 1× PBS (Sigma-Aldrich) and transferred into 15-ml centrifuge tubes, rinsed twice with cold 1× PBS and then incubate with the same volume of dissociation buffer with the addition of trypsin-EDTA (0.125%) at 37°C for 1 hr (Han et al., 2018a, b). scRNA-seq libraries were constructed as per the 10x Genomics protocol using GemCode Single-Cell 3′ Gel Bead and Library V2 Kit.
GEO Accession
GSM4090749