Argentina Pampean soils bacterial 16S rRNA pyrotags
DNA was isolated from 10 g of soil using the PowerMaxSoil DNA Isolation Kit following the manufacturer’s instructions (MO BIO Laboratories, Inc, CA, USA). Technical replicates were defined by performing up to 4 independent extractions per sample. A total of 42 DNA samples were used. The V4 hyper variable region of the 16s rRNA gene was amplified in duplicate PCR reactions using 520F and 802R bacterial universal primers. Amplicons were pooled and equimolar concentrations pyrosequenced at INDEAR (Argentina). on a Roche FLX 454 pyrosequencer running the Titanium chemistry.    All sequences have been submitted together. Here is a copy of QIIME mapping file used to demultiplex the samples so users can split the fasta and trim by quality into the 42 samples to perform local analysis:      #SampleID BarcodeSequence LinkerPrimerSequence ReversePrimer Sitio Manejo_1 Manejo_2 Description  leA2j10A ATACGACGTA CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC leA2 Agro SD leA2_jun_10_A  leA2j10B TCACGTACTA CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC leA2 Agro SD leA2_jun_10_B  leA2j10C CGTCTAGTAC CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC leA2 Agro SD leA2_jun_10_C  leA2j10D TCTACGTAGC CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC leA2 Agro SD leA2_jun_10_D  leNj10A ACTGTACAGT CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC leN No_Agro No_Agro leN_jun_10_A  leNj10B AGACTATACT CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC leN No_Agro No_Agro leN_jun_10_B  leNj10C AGCGTCGTCT CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC leN No_Agro No_Agro leN_jun_10_C  leNj10D AGTACGCTAT CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC leN No_Agro No_Agro leN_jun_10_D  lnAj10A CAGTAGACGT CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC lnA Agro SD lnA_jun_10_A  lnAj10B CGACGTGACT CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC lnA Agro SD lnA_jun_10_B  lnAj10C TACACACACT CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC lnA Agro SD lnA_jun_10_C  lnAj10D TACACGTGAT CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC lnA Agro SD lnA_jun_10_D  lnNj10A TACAGATCGT CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC lnN No_Agro No_Agro lnN_jun_10_A  lnNj10B TACGCTGTCT CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC lnN No_Agro No_Agro lnN_jun_10_B  lnNj10C TAGTGTAGAT CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC lnN No_Agro No_Agro lnN_jun_10_C  lnNj10D TCGATCACGT CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC lnN No_Agro No_Agro lnN_jun_10_D  ckAj10A TCGCACTAGT CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC ckA Agro SD ckA_jun_10_A  ckAj10B TCTAGCGACT CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC ckA Agro SD ckA_jun_10_B  ckAj10C TCTATACTAT CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC ckA Agro SD ckA_jun_10_C  ckAj10D TGACGTATGT CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC ckA Agro SD ckA_jun_10_D  ckNj10A TGTGAGTAGT CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC ckN No_Agro No_Agro ckN_jun_10_A  ckNj10B ACAGTATATA CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC ckN No_Agro No_Agro ckN_jun_10_B  ckNj10C ACGCGATCGA CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC ckN No_Agro No_Agro ckN_jun_10_C  ckNj10D ACTAGCAGTA CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC ckN No_Agro No_Agro ckN_jun_10_D  baSD1A AGCTCACGTA CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC baSD1 Agro SD baSD1_ago_10_A  baSD1B AGTATACATA CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC baSD1 Agro SD baSD1_ago_10_B  baSD2A AGTCGAGAGA CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC baSD2 Agro SD baSD2_ago_10_A  baSD2B AGTGCTACGA CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC baSD2 Agro SD baSD2_ago_10_B  baSD3A CGATCGTATA CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC baSD3 Agro SD baSD3_ago_10_A  baSD3B CGCAGTACGA CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC baSD3 Agro SD baSD3_ago_10_B  baLC1A CGCGTATACA CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC baLC1 Agro LC baLC1_ago_10_A  baLC1B CGTACAGTCA CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC baLC1 Agro LC baLC1_ago_10_B  baLC2A CGTACTCAGA CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC baLC2 Agro LC baLC2_ago_10_A  baLC2B CTACGCTCTA CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC baLC2 Agro LC baLC2_ago_10_B  baLC3A CTATAGCGTA CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC baLC3 Agro LC baLC3_ago_10_A  baLC3B TACGTCATCA CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC baLC3 Agro LC baLC3_ago_10_B  baNA1A TAGTCGCATA CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC baNA1 No_Agro No_Agro baNA1_ago_10_A  baNA1B TATATATACA CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC baNA1 No_Agro No_Agro baNA1_ago_10_B  baNA2A TATGCTAGTA CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC baNA2 No_Agro No_Agro baNA2_ago_10_A  baNA2B TCACGCGAGA CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC baNA2 No_Agro No_Agro baNA2_ago_10_B  baNA3A TCGATAGTGA CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC baNA3 No_Agro No_Agro baNA3_ago_10_A  baNA3B TCGCTGCGTA CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG CAGGAAACAGCTATGACC baNA3 No_Agro No_Agro baNA3_ago_10_B