GSM2360575: 082312_B6Cre_DL1_d12_FLDN2b_1; Mus musculus; RNA-Seq
Total RNA was extracted from 2.5∼20 million cells using Trizol (Invitrogen). The total RNA is subjected to two rounds of selection using Oligo-dT coupled magnetic beads (Dynabeads) according to the manufacturer's protocol.  cDNA library building was performed as described (Mortazavi et al., 2008). Briefly, mRNA was fragmented to an average length of 200 bp by Mg2+-catalyzed hydrolysis and then converted into cDNA by random priming. cDNA was then subjected to end repairing, adaptor ligation (using Illumina ChIP-seq sample preparation kit #IP-102-1001), size selection and one round of PCR amplification.
GEO Accession
GSM2360575