GSM2178296: CTCF_2_ChIP_COLO829; Homo sapiens; ChIP-Seq
Early passage cells were cross-linked in 1% formaldehyde and nuclei were extracted. Nuclei were lysed and DNA was sonicated for 6 hours in a Diagenode Pico Bioruptor (30 seconds on/ 30 seconds off). Chromatin was divided across two samples and incubated with polyclonal rabbit anti-CTCF antibody (Millipore 07-729) and rabbit immunoglobulin G (IgG; Sigma I5006) respectively. DNA was then purified, size selected and sequenced. Library construction was performed as per Illumina protocol Rev A 11257047
GEO Accession
GSM2178296