GSM3109274: WildType_DRIP-seq_IP; Saccharomyces cerevisiae; OTHER
DRIP protocol was performed as described in García-Pichardo et al. 2017. Briefly, harvested cells were treated to obtain spheroplasts, genomic DNA was extracted and spooled to purify it. Fragmentation was obtained with a cocktail of restriction enzymes. A fraction of the sample was separated as input control and the rest was immunoprecipitated using S9.6 monoclonal antibody. Both immunoprecipitated (IP) and input (INP) samples were subjected to a round of whole genome amplification using a GenomePlex® Complete Whole Genome Amplification Kit from Merck, following manufacturers specifications. Library was prepared to obtain a fragment size of 200bp according to manufacturer's protocol for the Ion Torrent PGM (Publication Part Number 4473623 Revision Date 14 November 2011 (Rev. A)). Samples were analyzed for size, integrity and contamination with Bionalyzer and High Sensitivity DNA analysis Kit at the Genomics service in CABIMER. Run was performed in Ion 316™ Chip v2. Barcodes were added in the library preparation step in order to pool them before loading the chip.
GEO Accession
GSM3109274