Illumina HiSeq 2000 sequencing; NOXlink_4SU_Rep1_C7F20ACXX_lane8
Protocols :  Extraction Protocol: Up to 10 million cells were lysed in 1 ml TRI reagent by pipetting up and down. For phase-separation 200 microl chloroform was added and samples mixed by turning tubes upside down several times. After 5 min incubation at room temperature samples were spun down with 12000 g for 10 min at 4C. Approx. 400 microl of the aqueous phase was transferred to a fresh tube, NaCl was added to a final concentration of 300 mM along with 1 microl GlycoBlue. Samples were combined with 500 microl isopropanol, mixed by inversion and RNA precipitated by centrifugation with 18000 g for 15 min at -10 C. The supernatant was removed and the RNA pellet washed with 1 ml of 70 % ethanol before resuspension in desired volume of nuclease-free water.  Library Protocol: Untranded RNA-seq library preparation. RRNA was NOT depleted using RiboZero (Illumina).
Factor Value[Growth Media]
DMEM+4SU
Factor Value[Treatment]
no UV