WGS of Escherichia coli O157:H7 str. EDL933: soil microcosm
DNA was extracted using a QIAamp DNA minikit (Qiagen, Hilden, Germany) with addition of an RNase A (100 mg/ml; Qiagen). A Nextera XT DNA sample preparation kit (Illumina, Inc., San Diego, CA) was used to prepare the library, and 2x75 bp paired-end reads were obtained by sequencing the library on the Illumina NextSeq platform.