Pl_Wa_H27_E_C.cornix_Pl_Wa_H27_tissue_Eye
"A total of 2o to 40 mg tissue was homogenized by mechanic disruption(TissueRuptor, Qiagen) in 1 ml TRIzol reagent(Invitrogen). Total RNA was then extracted using either the Qiagen RNeasy Mini Kit or RNeasy lipid tissue kit with the inclusion of an on-column DNA digestion step (RNase free DNase I, Qiagen)."