RNA-seq of Alligator mississippiensis: adrenal tissue of embryo incubated at MPT for 3 days
We incubated eggs at female-producing temperature (FPT, 30.0 deg C) until developmental stage 19. Then, we increased the incubation temperature of half of the eggs to male-producing temperature (MPT, 33.5 deg C) and incubated at both temperatures. We harvested eggs at either stage 19 or after 3 or 30 days of incubation after stage 19. We subdissected the gonad, adrenal, and mesonephros tissues and performed RNA-seq on each.