Illumina HiSeq 2500 sequencing; RNA-seq of Arabidopsis thaliana wild-type and a putative chloroplast ribosome biogenesis mutant AtRsgA homozygous knockout
RNA-seq of Arabidopsis thaliana wild-type and a putative chloroplast ribosome biogenesis mutant AtRsgA homozygous knockout
14 day old seedlings were harvested from the Murashige and Skoog medium plate.  No applicable. Seeds were surface sterilized and plated on Murashige and Skoog agar medium containing 1 % Suc (Murashige and Skoog, 1962) and grown in growth chamber under 16-h day (140 μmol m-2 s-1) / 8-h night regime.  No applicable. No applicable. RNA from seedlings was harvested using Spectrum Plant Total RNA Kit (Sigma-Aldrich).  TruSeq library protocol was used.
Experimental Factor: genotype
wild type genotype