GSM3401456: Dia c1 rep2a; Mus musculus; RNA-Seq
Mice were anesthetized and peripheral blood was flushed by cardiac perfusion using saline solution. Dura mater was removed, digested, and dural macrophages were sorted using BD FACS Aria II flow cytometer. RNA was harvested from bulk sorted dural macrophages using Qiagen RNeasy Plus Micro Kit (Cat# 74034). 1 µg of total RNA was used for the construction of sequencing libraries. RNA libraries were prepared using standard NEBNext® Ultra™ II RNA library prep kit protocols. All samples were sequenced with reads of 75 nucleotides, using a single-end library preparation (unstranded sequencing kit).
GEO Accession
GSM3401456