RNA-Seq of vehicle-treated mus musculus brain that is compressed/released
Quality of RNA samples was confirmed using a Fragment Analyzer (Advanced Analytical Technologies Inc.) before Illumina libraries were prepared and indexed from ~250ng of total RNA using the Kapa Hyperprep kit following manufacturer's recommendations (Roche). Libraries were confirmed using the Fragment Analyzer and quantified by qPCR prior to pooling and sequencing on an Illumina NextSeq500 using 40+40 paired end reads. Samples were demultiplexed using custom scripts allowing single mismatch to the index sequencing.