GSM2947687: WT RNA-Seq replicate 1; Mus musculus; RNA-Seq
RNA was extracted with RNeasy Plus Mini Kit (#74134 , Qiagen) for RNA-Seq. RNA libraries were make with Illumina Truseq stranded mRNA sample preparation kit following the manufacturer's recommended procedure. Quality control of the libraries were performed using Fragment Analyzer. The resulting libraries were pooled together. The pooled library concentration is further quantified using KAPA library quantification qPCR kit and sequenced on Illumina NextSeq 500 using a Highoutput 75 cycle sequencing kit targeting 20-30 million single read 75 bp reads.
GEO Accession
GSM2947687