GSM3577873: GBM4; Homo sapiens; RNA-Seq
Brain tumor-derived neurospheres were mechanically dissociated into single cells using a pipette and then loaded to a microwell-based chip (sc-FTDseq) for mRNA capture by uniquely barcoded beads. The cells were lysed using three cycles of freeze-thaw . The single cell mRNA libraries were prepared using the DropSeq protocol (GSE63473), tagged with Illumina nextera single indexes (N701, N702, N703, N704, N705, N706). We used the same protocol as in scFTD-seq(GSE109535), where each mRNA captured was end-paired with an unique molecular index(UMI) and a cell barcode. We prepared 2 to 4 biological repeats of each patient sample for library construction and sequenced 26,027 single cells in 26 batches.
GEO Accession
GSM3577873