Cold-stressed leaves 24h Olea europaea
the plants were incubated under the following cold stress conditions: 10ºC day / 4ºC night14-h photoperiod of fluorescent light at 65 µmoles.m2.s (10ºC day / 4ºC night) for 24 hours and constant 76-78% relative humidity. Time of cold exposure was considered from the moment at which the growth chamber reached the working temperature for the first time in daylight (i.e. 10ºC). RNA was extracted from leaves of three plants. Total RNA was extracted using a Spectrum Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO, USA) according to the manufacturer’s instructions. Any DNA contamination was removed by DNaseI treatment on column (Roche, Basel, Switzerland). The RNA-quality tests were performed with the Agilent 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) using an RNA 6000 Pico assay kit (Agilent Technologies, Santa Clara, CA, USA). We pooled RNA and made cDNA libraries for sequencing in Illumina HiSeq 1000 sequencer. Two replicates were sequenced on different lines. After trimming and cleaning, we obtained 69.681.545 paired-end reads of 101bp length and 35.2 Phred quality (14.07 Gb).