GSM4201862: TFB.expon.permanganate.ChIP-seq.r1; Saccharolobus solfataricus; ChIP-Seq
Cells were lysed in lysis buffer (50 mM HEPES/NaOH pH 7.5, 140 mM NaCl, 1 mM EDTA, 0.1% Na-deoxycholate, 1% Triton X-100) and DNA was sheared by sonication. Lysates were cleared by centrifugation. Chromatin-immunoprecipitation was carried out with protein-A-purified polyclonal antibodies. Immunoprecipitated complexes were treated ChIP-exo protocol using the ChIP-exo kit from Active Motif based on the protocol published by Serandour et al. (Genome Biology 14:R147 (2013) with the following modifications to adapt the protocol for permanganate ChIP-seq: lambda and RecJF exonuclease digestion steps were ommitted. Purified DNA was instead treated with 10% piperidine at 90 ºC for 30 min before 1-butanol and chloroform extractions. Second strand synthesis and all following steps were carried out according to the chip-exo protocol. Permanganate ChIP-seq
GEO Accession
GSM4201862