GSM1159717: Mbnl1-/-, reps 1-3; Mus musculus; OTHER
Quadriceps muscle was dissected from WT and Mbnl1-/- mice (15-16 weeks of age), snap frozen and powdered in liquid N2 and crosslinked with UV-light using a Stratalinker 1800 (Stratagene). Cell lysates were prepared and protein-RNA complexes were isolated by immunoprecipitation. Immunoprecipitation was performed using Mbnl2-specific antibody, mAb 3B4 and RNA tags were generated using RNase A concentrations of 38.6 U/ml and 0.0386 U/ml for high and low RNase, respectively. RNA tags bound to Mbnl2 were separated on a SDS gel, isolated, purified, precipitated and converted to cDNA libraries. These libraries were tagged with linkers and Illumina-specific sequencing adapters during the preparation and submitted for sequencing on the Illumina Genome Analyzer IIx.
GEO Accession
GSM1159717