Illumina paired whole exome sequencing of 3-Methylcrotonyl-CoA Carboxylase Deficiency study sample SAA10
Illumina TruSeq paired-end sequencing. Genomic DNA was DNA (2.5 ??g) was fragmented to ~175 bps using sonification (Covaris), fragment ends repaired and adaptors including sample index barcodes ligated, the resulting DNA libraries were amplified by 6 cycles of PCR, 500 ng enriched using SureSelect Human All v4 kit