L. pneumophila lab strain used at the start of the selection
We sequenced genomic DNA from the L. pneumophila isolates and a matched wild type strain as follows. DNA was prepared from each strain using a Purelink genomic DNA mini kit (Invitrogen, #K1820). DNA concentrations were quantified using Qubit and normalized to 0.5 ng/uL. Barcoded libraries were prepared using tagmentation according to Baym et al. 2015, analyzed with QuantIT DNA quantification, pooled at equimolar ratios, and sequenced with 50 bp paired-end reads on an Illumina HiSeq 2500 machine