16S Amplicon Sequencing of Swine Vaginal Microbial Communities
genomic DNA from each sample was amplified using Ampligold Taq polymerase (Applied Biosystems, Foster City, CA), with one replicate per sample using universal 16S rRNA gene bacterial primers  [515F (5-GTGYCAGCMGCCGCGGTAA-3; (Parada et al., 2016)) and (806R (5-GGACTACNVGGGTWTCTAAT-3; (Apprill et al., 2015))] for the variable region V4, as previously described (Kozich et al., 2013).