16S amplicon seq of Delia radicum eggs
Eggs were collected by flooding the eggboxes with (nonsterilized) demiwater, and collecting the runoff in a thin-necked volumetric flask, in order to concentrate the floating eggs at the top of the flask. They were then collected with a Whatman filter, and placed in a filter holder. Another whatman filter was placed on top and the eggs were washed with 5 mL of the following solutions using a syringe: milliQ water, 6.5% bleach, 70% ethanol, and milliQ water. Subsequently, the eggs were homogenized (filter and all) in LN2, and the dust was used downstream in DNA extraction using the Powersoil kit.