16S rDNA sequencing of human metagenome: necrotizing soft tissue infection site tissue biopsy
Tissue from necrotizing soft tissue infection site biopsies were obtained intraoperatively and stabilized in RNAlater (Thermo Fisher Scientific). Tissue biopsies were transferred into TRIzol (Invitrogen) reagent containing vanadyl ribonucleoside complex. Tissues were homogenized and cells were lysed with a beat beater. DNA extraction was based on the TRIzol method. Amplicon libraries of the V1-V2 region of the 16S rRNA gene were generated.