GSM4518441: Moffat_59_Renca_WT_TNFTx_replB; Mus musculus; RNA-Seq
RNA was extracted from cultured cells using the RNeasy Plus Mini Kit (QIAGEN) according to manufacturer's protocol. Briefly, cells were lysed on ice in RTL plus buffer (QIAGEN) and passed through QIAshredder columns. Supernatant was then transferred to RNeasy spin columns and an on-column DNase treatment was performed using the RNase-free DNase set (QIAGEN). 1000 ng per sample was processed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, E7760L) and included polyA-enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, E7490L), fragmentation for 15 minutes at 94°C prior to first strand synthesis, and 8 cycles of amplification after adapter ligation.
GEO Accession
GSM4518441