4C-Seq of mus musculus: adult female spleen
Resting B cells from lncCSR KO mouse spleen were isolated by negative selection using CD43 magnetic beads (Miltenyi Biotec) and cultured in RPMI1640 containing 15% FBS with 20 g/ml LPS (Sigma-Aldrich), 20ng/ml IL4 (PeproTech) and 1ng/ml TGF (R&D) for 24 hours. First restriction enzyme: HindIII; Second restriction enzyme: CviQI. Primer 1: GCATGTCTAGCAATAGGGTAAGCTT; Primer 2:TGACAGCCTCCTCCCAGAGTAC