Development of microsatellite markers in Schistura sikmaiensis using NGS technology
Genomic DNA was isolated from a single Schistura sikmaiensis individual collected from Khonglah, Meghalaya, India (25.14 N 92.00 E). DNA was prepared and sequenced (250 bp PE) on an Illumina Miseq by SciGenom Labs Pvt. Ltd.. A total of 402,942,305 reads were obtained after trimming and assembly. Following assembly, SSR markers were identified using MISA (MIcroSAtellite identification tool).