GSM2948497: Bdellovibrio with HEPES 3; Bdellovibrio bacteriovorus HD100; RNA-Seq
Overnight cultures of B. bacteriovorus grown on E. coli MG1655 were filtered (0.45 µM) to remove any residual prey or bdelloplasts. These cultures were mixed with either sterile HEPES or the S. aureus biofilm cultures. After 4 h at 30ºC, the B. bacteriovorus cells were filtered (0.45 µM) to remove any S. aureus, collected by centrifugation (16,000xg, 10 min) and the total cellular RNA extracted using ChargeSwitch Total RNA Cell Kits (Invitrogen) and thier manual After qualification of RNA samples, library was constructed via Hiseq 2500 (Illumina).   Libraries for sequencing were made using the Truseq Standard mRNA sample preparation kit (Illumina, USA). The sequencing was done using single-end 50 bp sequencing on the Illumina Hiseq 2500 platform.
GEO Accession
GSM2948497