GSM3261883: patient5-B; Homo sapiens; RNA-Seq
Total RNA was isolated from fresh frozen GC tissues using Trizol reagent (Invitrogen) according to the manufacture's protocol. The quality of RNA was assessed using Agilent 2200 TapeStation (Agilent technologies, US) to ensure high quality (RNA integrity number >6). Then, 1-2μg of total RNA was used for mRNA capture using NEBNext PolyA mRNA Magnetic Isolation Module and stranded RNA-seq libraries were constructed using a NEBNext Ultra Directional RNA Library Prep Kit.
GEO Accession
GSM3261883