Illumina HiSeq 2500 sequencing; Human BAT/WAT under Cold Exposure/Thermoneutrality
Human BAT/WAT under Cold Exposure/Thermoneutrality
Adipose tissue samples (100-150 mg) from the supraclavicular and abdominal areas were obtained using a suction adapted BergstrAsm needle, as previously described (Chondronikola et al., 2015).  Approximately 100 mg of adipose tissue was used for extraction of RNA using a pure link RNA isolation mini kit (Life Technologies, Carlsbad). RNA quality (RIN: RNA Integrity Number) was determined by Bioanalyzer (Agilent Technologies) and samples with at least RIN of 5 were subjected to RNA-seq. Libraries were constructed as previously described (Nature Medicine 21, 389a394 (2015))
Experimental Factor: Cold exposure
stimulus