GSM3263136: DMF_GSH_batch2_R3; Mus musculus; RNA-Seq
RNA was purified from Trizol according to the manufacturer's specifications. RNA was quantified with a Qubit 2.0 fluorometer (Invitrogen) and the quality was assessed on a Bioanalyzer 2100 (Agilent) using a RNA 600 Nano chip (Agilent) Samples with an RNA integrity number of > 9 were used for library preparation Barcoded mRNA-seq cDNA libraries were prepared with 300 ng of total RNA using NEBNext Poly (A) mRNA Magnetic Isolation Module and NEBNext Ultra RNA Library Prep Kit for Illumina according to the manual. Quantity was assessed using Invitrogen's Qubit HS Assay Kit and library size was determined using Agilent's 2100 Bioanalyzer HS DNA assay. Barcoded RNA-Seq libraries were onboard clustered using HiSeq Rapid SR Cluster Kit v2 using 8 pM and 50 bps were sequenced on the Illumina HiSeq2500 using the HiSeq RapidSBS Kit v2 (50 cycle)
GEO Accession
GSM3263136