GSM4146613: HIOEC day 3 rep3; Homo sapiens; ChIP-Seq
Cell were washed with ice-cold PBS (Hyclone) and fixed with 1% paraformaldehyde (PFA) for 10min at room temperature. PFA was then quenched in 134M Glycine (Sigma) for 5 min at room temperature, and cells were collected with a cell scraper in ice-cold PBS. After centrifuge for 10 mins at 500g, the cell pellets were resuspended with 5mM PIPES pH8.5, 85mM KCl, 1% (v/v) IGEPAL CA-630, 50mM NaF, 1mM PMSF, 1mM phenylarsine oxide, 5mM Sodium Orthovanadate and protease inhibitor cocktail (Roche, Germany). After sonication, chromatin immunoprecipitation was performed using 4ug of anti-Histone H3, Acetylated Lysine 27 (H3K27Ac) (Abcam, Cambridge, UK, ab4729)  per 500,000 cells. ChIP-seq library were indexed with kit (ND606-01, Vanzyme, China)
GEO Accession
GSM4146613