DNA-Seq of Toxoplasma gondii strain CZ clone H3
Genomic DNA from Toxoplasma gondii strain CZ clone H3 was extracted from tachyzoites using the QIAamp DNA Mini Kit (QIAGEN). DNA was sheared with the Covaris Adaptive Focused Acoustics and size selected with AMpure beads (Beckman Coulter), end-repaired and polyadenylated. Libraries were generated using the TruSeq HT DNA NanoSample Prep Kit v2 (Illumina Inc.), and cluster generation was performed using the Hiseq 4000 PE Cluster Kit (Illumina Inc.).