GSM2359897: C2; Ovis aries; RNA-Seq
MSC were pelletted from 250 ml of fresh milk at 1100g for 10 min at 4°C. The cell pellet was washed twice in PBS, pH 7.2 with 0.5 mM EDTA and resuspended in 3 ml of Trizol Reagent (Life Technologies). RNA extraction continued following the Trizol protocol, performing all centrifugation steps at 4°C and isopropanol precipitation at -80°C for 30 min. A DNase I (Roche) treatment was performed according to the manufacturer’s instructions, to completely remove genomic DNA contamination. Finally RNA was purified by phenol/chloroform extraction and precipitated following standard procedures. RNA-Seq library was generated using the TruSeq RNA-Seq Sample Prep kit, according to the manufacturer protocol (Illumina Inc., San Diego, CA, USA)
GEO Accession
GSM2359897