GSM4088877: MOLM13-Cas9_CUTRUN_MLL1_sgNull_d6_REP2_190710_CGATGT_S2; Homo sapiens; OTHER
The cells were collected and washed twice in ice-cold PBS. The cells were immobilized on Concavaline-A magnetic beads; followed by permealization pf cells with Triton-X100. Immobilized cells were incubated with anti-MLL1n antibody for 2 hours, washed, and incubated with ProteinA-MNase conjugate for 4 hours. After two washes MNase was activated by addition of 2mM CaCl2 for 30 min. Released DNA fragments were collected in supernatant and purified using AmpureXP beads 1-10 ng of ChIP'ed DNA was used for Illumina compatibl library generation using NEB Ultra II kit with 12 cycles of amplification with single end barcodes Cut&Run-seq using NEB Ultra II protocol
GEO Accession
GSM4088877