GSM1446259: S2A; Schizosaccharomyces pombe; MNase-Seq
Chromatin was prepared from formaldehyde crosslinked log phase cells and digested with micrococcal nuclease (Fermentas #ENO181) to a final concentration of 180 units/ml (WT) and 300 units/ml (S2A mutant) for 40 minutes at 37 ºC to yield about 90% mononucleosomes. After reverting crosslink DNA of mononucleosomal size was purified from agarose gels Mononucleosomal DNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM1446259