Meta-Seq of Manis javanica: adult female A-2
Qulified DNA samples were sheared into smaller fragments by nebulization firstly. Then the overhangs resulting from fragmentation are converted into blunt ends by using T4 DNA polymerase, Klenow Fragment and T4 Polynucleotide Kinase. After adding an A(adenine) base to the 3' end of the blunt phosphorylated DNA fragments, adapters are ligated to the ends of the DNA fragments. Then short fragments are removed with Ampure beads. Agilent 2100 Bioanaylzer and ABI StepOnePlus Real-Time PCR System were used to qualify and quantify the sample libraries. The qualified libraries were then sequenced via Illumina HiSeq platform.