DNA-seq of Canis lupus familiaris: adult female blood
Whole-genome sequencing was performed by Omega Bioservices (Norcross, GA, USA). DNA concentration was measured using the QuantiFluor dsDNA System on a Quantus Fluorometer (Promega, Madison, WI, USA). A Kapa Biosystems HyperPrep kit (Kapa Biosystems, Wilmington, MA, USA) was used for whole-genome library construction. Briefly, 1 ug of genomic DNA was fragmented using a Bioruptor sonicator (Diagenode, Denville, NJ, USA). DNA fragment ends were repaired, 3 adenylated, and ligated to adapters. The resulting adapter-ligated libraries were PCR-amplified, Illumina indexes added, and pooled for multiplexed sequencing on an Illumina HiSeqX10 sequencer (Illumina, San Diego, CA, USA) using the pair-end 150bp run format.