rice blast fungus Whole genome seq.
genomic DNA was randomly sheared into short fragments of about 350 bp. The obtained fragments were subjected to library construction using the Illumina TruSeq Library Construction Kit, with strictly following the instructions. Briefly, as followed by end repairing, dA-tailing and further ligation with Illumina adapter, the required fragments (in 300-500 bp size) with both P5 and P7 sequences were PCR selected and amplified. After gel electrophoresis and sub sequent purification,the required fragments were obtained fo r library construction.