GSM3262746: Mi-R3-4: Host-Race-3 (PR-3, MTS-R3, BA-R3), Cotton, Pool of geographical locations; Meloidogyne incognita; OTHER
We extracted and purified the genomic DNA from eggs of each nematode isolate with the supplement protocol for nematodes of the QIAGEN Gentra® Puregene® Tissue Kit with the following modifications: incubation at 65 ° C in the cell lysis buffer for 30 min and incubation at 55 ° C with proteinase K for 4h. Libraries were pooled at 4nM, loaded on Nextseq 500 High Output Flowcells and sequenced on a NextSeq 500 platform (Illumina) with 2 × 150bp paired-end chemistry.
GEO Accession
GSM3262746