GSM3881041: sperm, Famine F0_rep1; Mus musculus; MeDIP-Seq
Sperm were removed, incubated with lysis buffer at 55℃ for 3h under constant rotation, then extracted using TIANamp Genomic DNA Kit according to the manufacture's instructions. DNA was sheared by sonication and fragmented to a size of 100-500 bp, then immunoprecipitated with antibody against 5-mc, then purify immunoprecipitated DNA. Libraries were constructed with TruSeq DNA library preparation kit (Illumina)
GEO Accession
GSM3881041