Illumina MiSeq paired end sequencing of SAMD00089856
The V4 region of the 16S rRNA gene was amplified by PCR with the primer set with the appropriate Illumina adaptor, an 8-nt index sequence, a 10-nt pad sequence, a 2-nt linker, and the gene-specific primer. The PCR products were electrophoresed and purified using a QIAquick PCR Purification Kit (Qiagen, Valencia, CA, USA) to remove unreacted primers. Amplified PCR products were sent to the Next Generation Sequence service using Illumina platform performed by FASMAC, Japan.