GSM4296111: Sample 3, ILW muscle, mRNA; Sus scrofa; RNA-Seq
Total RNA was extracted with Trizol (Invitrogen) according to the manufacturer's instruction. Results of the extraction were quantified using a Nanodrop ND-1000 spectrophotometer (Nanodrop Technologies) and the quality of the extracted RNA was assayed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Stranded total RNA libraries were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) following the manufacturer's suggested protocol. The libraries were tagged, and pairs of libraries were run on a single lane of an Illumina HiSeq2500 (Illumina). Paired-end reads of 100 bp were generated.
GEO Accession
GSM4296111