GSM1446250: FTO1C1_me6A; Homo sapiens; RIP-Seq
The large and small RNA fractions from FTO knockdown was prepared using RNeasy Plus Universal mini kit (Qiagen, Hilden, Germany) following the instructions of the manufacturer. The small RNA fraction was eluted with the RNeasy MinElute Cleanup Kit (Qiagen, Hilden, Germany). Illumina TruSeq Small RNA Sample Preparation Guide was used for cDNA synthesis and library preparation. The protocol was strictly followed. Briefly, 3’ and 5’ adapters were ligated to the samples, followed by reverse transcription and amplification with primers containing the unique indices for different samples. Amplified cDNA constructs were purified using Ampure XP beads. DNA was loaded into two lanes at 5 pM and 10 pM concentrations and subjected to Illumina sequencing. Demultiplexing was done using Illumina CASAVA 1.8.2.
GEO Accession
GSM1446250