Cystic_fibrosis_Epithelial_cells_vehicle_1_CF225
Bronchial brushings were obtained from the fourth- to sixth-generation airways from patients with cystic fibrosis during clinical exacerbation and placed directly immediately into Qiagen RLT plus buffer during the bronchoscopy to ensure minimal RNA degradation. Samples were then stored at ?80°C until RNA isolation using Qiagen RNeasy isolation kit. RNA quantity and integrity were determined using Agilent TapeStation 2200 and Total RNA was used as starting material for deep sequencing using the Illumina TrueSeq RNA Sample Preparation v2 library preparation kit. Cluster generation and 75 bp single-end dual-indexed sequencing was performed on Illumina NextSeq 500