GSM4706315: ALUBIA SC15 #1; Phaseolus vulgaris; RNA-Seq
RNA was extracted with Trizol following manufacture´s recommendations (Thermo Fisher). RNA quality was evaluated by capillary gel electrophoresis on an Agilent 2100 Bioanalyzer using the AgilentRNA6000 Pico kit. Libraries were prepared using 3 µg of RNA and the Illumina TruSeq RNA Sample Preparation kit v2 following provider's instructions. Superscript II (Invitrogen) reverse transcriptase was used for cDNA synthesis. PCR fragments were purified using AMPure XP beads (Beckman Coulter Genomics). Size of the synthesized fragments was verified in an Agilent 2100 Bioanalyzer using the DNA-1000 kit (Agilent). Libraries were multiplexed following Illumina recommendations for barcode compatibility.
GEO Accession
GSM4706315