GSM3883428: LCP_D10_rep1; Mus musculus; RNA-Seq
Cochlear sensory epithelia were dissected out and incubated with Cell Recovery Solution (Corning) to separate hair cells and supporting cells from mesenchyme and neurons. RNA was extracted using RNeasy Micro Kit (Qiagen) from hair cells and supporting cells alone. Every sample is from 2 ears of 1 pup. cDNA was synthesized from 2.5 ng of RNA using SMARTer V4 Kit (Clontech). Fragmentation was done using M220 Focused-Ultrasonicator (Covaris) and 2 ng of sheared cDNA were taken for library preparation using ThruPLEX DNA-seq kit (Rubicon Genomics) NextSeq500 Single-End 75bp (SE75) Sequencing (Illumina)