GSM4454147: LLC_CON_1; Mus musculus; RNA-Seq
Total RNA was extracted from mice tissues with TRIzol (Invitrogen). RNA library preparation was performed as described in the QIAseq Stranded RNA Library Kits (QIAGEN). The mRNA was fragmented to an average insert size of 200–400 bp. The cleaved RNA fragments were copied into first-strand cDNA using reverse transcriptase (Thermo Fisher Scientific) and random primers. The first-strand cDNA was converted into double-stranded DNA in the presence of dUTP. These cDNA fragments were subjected to the addition of a single 'A' base and subsequent ligation of the adapter. The products were purified and enriched via PCR to generate the final library. After testing quality using Qubit2.0 Fluorometer and Agilent 2100 Bioanalyzer (Agilent Technologies), the libraries were sequenced on the Illumina HiSeq platform (Illumina Inc., San Diego, CA, USA).
GEO Accession
GSM4454147