GSM4023618: Replicate2_1; Mus musculus; RNA-Seq
See 10X Genomics V2 Single Cell 3′ Solution kit user guide For 10X libraries, see 10X Genomics V2 Single Cell 3′ Solution kit user guide. Amplicon sgRNA libraries were performed on full length 10X cDNA samples as previously described (Hill et al. 2018); briefly, 10 ng of full length 10X cDNA were used as starting material and each round of PCR amplification with the primers described in (Hill et al. 2018) was monitored by KAPA SYBR to avoid overcycling. Each 10X library was sequenced on an Illumina HiSeq4000 lane with 26 cycles for read 1, 98 cycles for read 2 and 8 cycles i7 sample index. The 24 amplicon libraries were multiplexed and sequenced across two lanes of an Illumina Hiseq2500 Rapid Run, with 27 cycles for read 1, 267 cycles for read 2 and 8 cycles for i7 sample index.
GEO Accession
GSM4023618