GSM3771321: MOLM16_SD36_8h_2; Homo sapiens; RNA-Seq
Cells were collected at the end of treatment and total RNA was purified using RNeasy Mini Kit from Qiagen. RNA was assessed for quality using the TapeStation (Agilent). Samples with RINs (RNA Integrity Numbers) of 8 or greater were ribosomal RNA depleted using Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat) (Illumina). RNA libraries were prepared from the rRNA-depleted total RNA using TruSeq RNA Library Prep v2 kit (Illumina) following Illumina's standard protocol. Where the entire fraction of 0.1-3ug of rRNA depleted total RNA was fragmented and copied into first strand cDNA using reverse transcriptase and random primers. The 3 prime ends of the cDNA were then adenylated and adapters were ligated. One of the adapters that was ligated had a 6 nucleotide barcode that was unique for each sample which allowed us to sequence more than one sample in each lane of a HiSeq flow cell (Illumina). The products are purified and enriched by PCR to create the final cDNA library. Final libraries were checked for quality and quantity by TapeStation (Agilent) and qPCR using Kapa's library quantification kit (Kapa Biosystems) for Illumina Sequencing platforms.
GEO Accession
GSM3771321