DNA-Seq of dairy cattle: adult female rumen fluid
The V4 region of the bacterial and archaea 16S rRNA gene was amplified using the universal primers 515F (5 -GTGCCAGCMGCCGCGGTAA-3 ) and 806R (5 - GGACTACHVGGGTWTCTAAT-20 )  with forward primers tagged with unique barcode sequences for each sample.