454_eukarya_soil_biofumigation_experiment
0.5 g of soil were used for DNA extraction through the bead-beating method using FastDNA SPIN Kit. The amplification of the eukaryotic 18S rRNA gene was carried out using the universal primers FF390 and FR1. Amplicons were purified using the Pure Link TM Quick PCR purification kit (Invitrogen). Forward 515F primer was equipped with the Roche 454-A pyrosequencing adapter and a TCAG linker, while 806R primer had the Roche 454-B sequencing adapter and a TCAG linker. Each forward primer contained a 12-bp barcode.