GSM2358127: Treated Timepoint 1; Homo sapiens; RNA-Seq
For RNA-Seq, total RNA was extracted using the Qiagen RNeasy kit (Qiagen) and treated with RNAse-free DNase (Qiagen). RNA from ~2 million cells was extracted, with concentration measured on a Qubit (Life Technologies). Additionally, RNA integrity was checked on a Bioanalyzer (Agilent, Santa Clara CA) and only RNA with an RNA integrity number (RIN) of > 8.5 was used for subsequent library construction. Libraries were constructed using Illumina's TruSeq kits with Ribo-Zero following the manufacturer's protocol. cDNA was quantitated on a Qubit (Life Technologies) and additionally the library quality was evaluated on a Bioanalyzer (Agilent)
GEO Accession
GSM2358127