GSM3446420: CD11c saline wafergen [133]; Mus musculus; RNA-Seq
Islets isolated from the pancreases of NOD mice by digest with 0.8% Collagenase P for 11-14 minutes at 37 degrees Celcius, seperated with density gradient, and hand picked. Islets were digested with4 Wunsch units of Collagenase D (Roche) with 250 μg/ml DNAse in HBSS with 10% FBS followed by Cell Dissociation Buffer (Sigma)at 37 degrees Celcius for 30 minutes each. Sample RNA was isolated using the Quick-RNA Microprep kit (Zymo Research) according to the manufacturer's protocol. RNA AmpliSeq libraries were constructed and barcoded with the Ion AmpliSeq Transcriptome Mouse Gene Expression Kit and methods. Amplicon RNA-seq, Unique mapping option
GEO Accession
GSM3446420