GSM2598434: tDNA2334_rep1; Saccharomyces cerevisiae; OTHER
DNA was purified by PCI extraction and ethanol precipitation, treated with RNase A, and ~250-350 bp DNA was gel-purified following 3% agarose gel electrophoresis. Purified DNA was treated with End-it, subject to A-tailing with Exo- Klenow, and ligated to Illumina adaptors. Adaptor-ligated DNA was then purified with streptavidin beads to isolate ligated Micro-C products away from undigested dinucleosomal DNA. Streptavidin beads were then subject to ~10-12 cycles of PCR using Illumina paired-end primers. Amplified library was purified and subject to Illumina Nextseq 500 paired end sequencing with 42 x 42 bp reads.
GEO Accession
GSM2598434