Amplicon sequencing of Activated Sludge community
DNA extraction, sample preparation including amplification of V1-3 region of 16S rRNA gene using the 27F (AGAGTTTGATCCTGGCTCAG) (Lane, 1991) and 534R 194 (ATTACCGCGGCTGCTGG) (Muyzer et al., 1993) primers, and amplicon sequencing were conducted as described by Stokholm-Bjerregaard et al., (2367)