GSM2177790: WT_NP; Mus musculus; RNA-Seq
Total RNA was extracted from J1 and Dnmt3 double KO ESCs. 5 μg of RNA was subjected to rRNA depletion, fragmentation, and cDNA synthesis following the TruSeq RNA Sample Preparation guide (Illumina). End repair, addition of a single dA, ligation with indexed adaptors, and 15 cycles of PCR with Illumina primers were performed also following the TruSeq RNA Sample Preparation guide. RNA-seq library was purified using AMPure XP beads (Agencort) and quantified its size distribution on a TapeStation Instrument (Agilent Technologies). Single-end sequencing was performed on Illumina HiSeq 2000 with 50 cycles.
GEO Accession
GSM2177790