Illumina HiSeq 1500 sequencing; RNA-seq of D. melanogaster S2 cells upon depletion of dCoREST and its interacting partners compared to control
RNA-seq of D. melanogaster S2 cells upon depletion of dCoREST and its interacting partners compared to control
S2 cells were collected after 6 days of knock down by double stranded RNAs targeting dCoREST and its interactors. D. melanogaster S2 cell were maintained in Schneider's medium (Gibco) supplemented with 10% (v/v) fetal calf serum (BiochromAG) and 1% (v/v) Penicillin-Streptomycin (Gibco) under standard conditions (26 °C). S2 cells were treated with double stranded RNAs targeting EGFP (control), dCoREST, dCoREST-L, dLSD1, dL(3)mbt, dLint-1 and dG9a. Total RNA from Drosophila S2 cells was isolated using the peqGOLD Total RNA Kit (S-Line, Peqlab) according to manufacturer's instruction. RNA-seq libraries were prepared using a TruSeq Stranded mRNA Library Prep kit (Illumina).
Experimental Factor: RNA interference
dLint-1 knockdown by siRNA