GSM1980036: JU1421 rep3; Caenorhabditis nigoni; RNA-Seq
300 young adult males were picked for mRNA extraction for each sample for C. briggsae (AF16), C. nigoni (JU1421), ZZY10307 and ZZY10330 with three replicates each. For collection of ZZY10307 and ZZY10330 young adult males, fertile females that carry the introgression were mated with JU1421 males. The male progeny that carry the introgression as judged by the expression of cbr-myo-2::GFP were picked under a fluorescence microscope for mRNA extraction. Total RNA was extracted using TRIzol (Invitrogen) following the manufacturer’s instructions. Illumina’s TruSeq Stranded mRNA Library Preparation Kit was used with 300 ng of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using Illumina’s TruSeq Stranded mRNA Library Preparation Kit based on the Kit’s manual
GEO Accession
GSM1980036