GSM3399976: Ananascomosus_I378-J03_good; Ananas comosus; Bisulfite-Seq
DNA was extracted from 'D' leaf of pineapple (Ananas comosus var. comosus cultivar MD-2), and BS-seq libraries were prepared using the TruSeq Nano DNA LT kit (Illumina), as described previously. For each tissue type, two libraries corresponding to two biological replicates were sequenced on a HiSeq X Ten system (Illumina) to obtain paired-end 150-bp reads per the manufacturer's instructions. Total RNA was extracted from the same tissue used for the BS-seq libraries, and RNA-seq libraries were prepared using the TruSeq Preparation Kit with polyA mRNA selection, per the manufacturer's instructions (Illumina). Two libraries were pooled and sequenced to obtain paired-end 150-bp reads on the Illumina HiSeq X Ten system.
GEO Accession
GSM3399976