RNA-Seq of mus musculus: pup calvaria
Primary microRNA and mRNAs were captured by Dynabeads Oligo (dT)25 magnetic beads (Invitrogen) and fragmented by using the NEBNext Magnesium RNA Fragmentation Module (New England Biolabs). Double-stranded cDNAs were synthesized with SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen) and were used as the library template. Libraries were generated with TruSeq RNA Library Preparation Kit (Illumina). Sequencing was performed on an Illumina HiSeq 2000 instrument as 100 bp pair-end reads conducted at the Human Genome Sequencing Center at Baylor College of Medicine