GSM3105260: fast_iWAT_ATACseq_rep1; Mus musculus; ATAC-seq
The fat tissue was minced with blades, dounced in homogenization buffer (20mM Tricine pH7.8, 25mM D-Sucrose, 15mM NaCl, 60mM KCl, 2mM MgCl2, 0.5mM Spermidine), filtered with EMD Millipore Nylon-Net Steriflip™ Vacuum Filter Unit (100um), and centrifuged at 18000g for 5min at 4C. The resulting nuclei pellet was washed once with Tagmentation DNA buffer (10mM Tris pH7.6: acetic acid, 5mM MgCl2, 10% dimethylformamdie) followed by centrifugation at 500g for 5min at 4C. After resuspension in the same Tagmentation DNA buffer and counting using Trypan Blue, an aliquot contacting 50K nuclei was performed transposition reaction using Nextera DNA Library Preparation Kit (illumina) and processed as descripted.
GEO Accession
GSM3105260