GSM3109192: Pex11BirA_2minCHX_2minBiotin_input; Saccharomyces cerevisiae; OTHER
Yeast cells were harvested by filtration, flash frozen, and cryogenically pulverized in a Retsch mixer-mill. Footprinting was performed with RNase I, monosomes purified on 10-50% sucrose gradients and biotinylated ribosomes purified on streptavidin dynabeads. RNA was extracted with Trizol and protected fragments gel purified. Ends were repaired with T4 PNK and ligated to a pre-adenylated linker with T4 RNA Ligase 2. Unligated linkers were enzymatically removed and ligated fragments were gel purified, reverse transcribed and gel purified. The cDNA was circularized then PCR amplified.
GEO Accession
GSM3109192