GSM4145174: ∆Ms_1939_2; Mycolicibacterium smegmatis MC2 155; RNA-Seq
Total RNA was isolated using RNeasy mini kit (Qiagen, Germany). Paired-end libraries were synthesized by using the TruSeq® RNA Sample Preparation Kit (Illumina, USA) following TruSeq® RNA Sample Preparation Guide. The cDNA fragments afterwards through an end repair process, the addition of a single 'A' base, and then ligation of the adapters. The products are then purified and enriched with PCR to create the final cDNA library.
GEO Accession
GSM4145174