Illumina HiSeq 1500 sequencing; RNA-seq of Drosophila melanogaster testes upon knock-down of dCoREST, dLSD1 and controls
RNA-seq of Drosophila melanogaster testes upon knock-down of dCoREST, dLSD1 and controls
2-3 days old fly testes were dissected from flies Total RNA from dissected Drosophila testes was isolated using the TRIzol (Invitrogen) reagent according to the manufacturer's protocol. Following chloroform extraction, ethanol precipitation, and DNase digestion, RNAs were purified using an RNeasy Mini Kit (Qiagen). RNA-seq libraries were prepared using a TruSeq Stranded mRNA Library Prep kit (Illumina). Libraries were quantified on a Bioanalyzer (Agilent Technologies).
Experimental Factor: genotype
bam-Gal4/w1118;Sp/P{KK102965}VIE-260B;bam-Gal4:VP16/+
Experimental Factor: phenotype
dLSD1 knockdown by siRNA