DNA-Seq of Homo Sapien: Duodenum
Bulk amplification of T-cell receptor repertoires was undertaken with the Biomed-2 kit (Invivoscribe) for TCRD(TRD), using 150ng DNA input. An equal amount of all purified amplicons was pooled into a library of 4 nM, denatured, diluted, loaded on to the MiSeq, and subjected to MiSeq run (v3, 2  300 cycles; Illumina, San Diego, CA)