GSM2087110: CstF64 KD rep 1; Homo sapiens; RNA-Seq
HeLa cell lines were stably transfected with shRNA plasmids targeting CstF64 and maintained by culturing in the presence of 1 mg/ml puromycin. Total RNA was isolated from CstF64 KD and wild-type control cells using Trizol reagent. Total RNA isolated from two biological replicates was used to generate independent libraries using the Illumina TruSeq RNA sample preparation kit according to manufacturer’s protocols. Each library was diluted to approximately 10 pM prior to loading and sequencing on the Illumina GA IIx instrument.
GEO Accession
GSM2087110