GSM3878594: p38_KO-flu_rep2; Caenorhabditis elegans; RNA-Seq
Total RNA was extracted using the Direct Zol RNA mini perp kit (Zymo R2070) and was treated with DNase, according to the manufacturer's instructions. The concentration of the mRNA was quantified by both Nanodrop and Qubit (Thermo Fisher Scientific), and RNA was used for library construction and the RNA sequencing procedure. RNA libraries were prepared using the TruSeq RNA Library Prep Kit v2 (Illumina), according to the manufacturer's protocol. The libraries were loaded on one lane of an Illumina HiSeq 2500 instrument and were ran by 50 bp single read using V4 reagents at the Technion Genome Center, Technion¬Israel Institute of Technology.
GEO Accession
GSM3878594