SD mtx3 male TIDC
Total RNA from DC from tumors, was extracted with RNeasy Micro Kits (Qiagen, Hilden,Germany), following the manufacturers instructions. The concentration and quality of RNAsamples were determined by NanoDrop 2000 spectrophotometer (NanoDrop technologies, USA) and a RNA LabChip 6000 Nano kit (Agilent Technologies, USA). DC samples with atotal RNA concentration above 50ng were used for RNAseq