Paired end Sequencing of Cryptococcus  gattii VGIIb WM 16.20
DNA for WGS was extracted using the Quick-DNA Fungal/Bacterial Miniprep kit (Zymo Research, Irvine, CA, USA) according to the manufacturers instructions. Genomic DNA then was fragmented using a Q800R2 sonicator (QSonica, Newtown, CT, USA) to approximately 500bp, and genome libraries were prepared for paired-end sequencing using the NEBNext Ultra II DNA Library Prep kit (New England BioLabs, Ipswich, MA, USA) and quantified using the SequalPrep Normalization Plate kit (Thermo Fisher Scientific, Waltham, MA, USA). Libraries were pooled and sequenced at 2150 bp on a NextSeq (Illumina, Inc., San Diego, CA, USA).