Illumina HiSeq 2500 paired end sequencing; RNA-seq of the Ileo-Caecal Valve Lymph Node of Sheep with Paratuberculosis Compared to Uninfected Controls.
RNA-seq of the Ileo-Caecal Valve Lymph Node of Sheep with Paratuberculosis Compared to Uninfected Controls.
At post-mortem the ileo-caecal lymph node (ICLN) from either diseased sheep with Paratuberculosis or uninfected control sheep were removed. The ICLN was treated with RNAlater RNA Stabilization Solution (Ambion) and stored at -80°C prior to RNA extraction (according to manufacturer’s instructions). Total RNA was extracted from the homogenized samples using a TRIzol ® Plus RNA Purification Kit (Ambion) as described by the manufacturer including DNase I treatment. RNA libraries were prepared by Edinburgh Genomics as per their standard protocol
Experimental Factor: Multibacillary Paratuberculosis
disease