GSM1826512: Bulk cells_MMTV-Myc cells; Mus musculus; RNA-Seq
Total RNA was extracted with the QIAGEN RNeasy Mini Kit RNA samples were quality checked (RIN score of 8 or higher) using the RNA 6000 Nano chip on a 2100 Bioanalyzer (Agilent). mRNA was then isolated using Sera-Mag oligo-dT beads (Thermo Fisher Scientific), and cDNA library was synthesized by SuperScript II (Invitrogen) with random primers. The cDNA library was quality checked. Pooled libraries were loaded on high throughput HiSeq 2500 flow cells, using v3 reagents, and sequenced 100nt, single-end.
GEO Accession
GSM1826512