Illumina HiSeq 3000 paired end sequencing; RNA-Seq of chicken breast samples treated with chronic heat stress
RNA-Seq of chicken breast samples treated with chronic heat stress
Samples were collected 3-hour, 2-weeks, and 4-week post heat from both control (unheated) and treatment (heated) birds.  Samples were immediately placed in RNAlater post collection. Daily heat cycles consisting of 7 hours of elevated temperature of 35°C and 17 hours of ambient temperature of 30°C for treatment birds. Control birds remained at 23°C throughout the entire experiment. RNAqueous Total RNA Isolation Kit following manufacturer's standard protocol and treated with Ambion DNA-free DNase Treatment and Removal Reagents following manufacturer's standard protocol. Illumina TruSeq Stranded mRNA Library Prep Kit following the manufacturer's standard protocol.
Experimental Factor: environmental stress
none
Experimental Factor: time
3