GSM4454163: 4.2_DMSO_d6_H3K4me1; Homo sapiens; OTHER
CUT&RUN was performed according to the high-calcium/low-salt digestion protocol described previously (Meers et al. 2019). The antibodies used in our CUT&RUN studies are anti-rabbit IgG isotype (ABIN101961, Antibodies online, 1:50), anti-H3K4me1 (C15410194, Diagenode, 1:50), anti-H3K27ac (ab4729, Abcam, 1:50) and anti-H3K27me3 (9733, CST, 1:50). CUT&RUN libraries were prepared with the NEBNext® Ultra II DNA Library Prep Kit.
GEO Accession
GSM4454163