Illumina HiSeq 2500 paired end sequencing; Bulk RNA-Seq of whole testis sampled from juvenile mice
Bulk RNA-Seq of whole testis sampled from juvenile mice
Testes from prepubertal C57/BL6J mice ranging between postnatal day 6 and 35 were flash frozen or directly used for RNA extraction using Trizol (Thermo Fisher, 15596026) following manufacturer's instructions. Purified RNA was DNase-treated using the TURBO DNA-free Kit according to manufacturer's instructions (Thermo Fisher, AM1907) and RNA quality was assessed using the Agilent Tapestation RNA Screentape.  800ng of DNA-depleted RNA were used for RNA-Seq library preparation using the TruSeq Stranded Total RNA Library Kit with Ribo-Zero Gold for cytoplasmic and mitochondrial ribosomal RNA removal according to manufacturer's instructions (Illumina, RS-122-2303).
Experimental Factor: age
35