RNA seq of fire ant queen brains
sample QC, ribodepletion, cDNA synthesis automated Illumina TruSEQ stranded RNA_SeqLibrary construction on the Biomek liquid handling platform, library QC via TapeStation and qPCR, detection of sequencingfragments using the Illumina HiSeq 2500 platform, basecalling, primary data QC, demultiplexing and FASTQ file generation