RNA-Seq of Mus musculus brain
The RNeasy Lipid Tissue Mini Kit (Qiagen) was used to extract total RNAs from the brainstem of five animals in each experimental group. The RNAs were precipitated and their quality and quantity assessed using an Agilent Bioanalyzer 2100 and an RNA 6000 chip (Agilent Technologies). The cDNA libraries were then constructed using the TruSeq RNA Sample Prep Kit v2 (Illumina, Inc.) and their quality checked using an Agilent Bioanalyzer 2100 and a DNA 1000 chip (Agilent Technologies). The libraries were Paired End sequenced in a HiSeq 4000 system (Illumina, Inc.). We aimed for 45 Giga Bases outcome per sample.