Illumina HiSeq 2500 sequencing; Genome-wide characterisation of DNA methylation in an invasive Lepidopteran pest, the cotton bollworm Helicoverpa armigera
Genome-wide characterisation of DNA methylation in an invasive Lepidopteran pest, the cotton bollworm Helicoverpa armigera
Samples snap frozen in liquid nitrogen and stored at -80C Insects reared at 25-27 degrees celsius and 14h:10h light:dark cycle in controlled environment 100ng DNA bisulfite converted using the EZ DNA Methylation-Lightning Kit DNA extracted using the DNeasy Blood & Tissue kit (QIAGEN) Bisulfite-converted DNA was amplified with a primer that contained part of an adaptor sequence plus four random nucleotides followed by two additional amplifications to add on the remaining adaptor sequence and to barcode the fragments. PCR products were purified using the DNA Clean & Concentrator-5â„¢ (Zymo Research).
Experimental Factor: organism
Helicoverpa armigera