GSM4088948: DN_3; Mus musculus; RNA-Seq
Aortas and carotid arteries from male and female Apoe-/- Cx3cr1GFP Cd11cYFP fed WD for 5 months were enzymatically digested. Single cell suspensions were stained with CD45 and 7AAD. Live, CD45+ cells were flow sorted into GFP+ YFP- (GFP), GFP+YFP+ (DP), GFP-YFP+(YFP), and GFP-YFP- (DN) groups using a FACSAria II directly into Trizol LS. RNA was extracted from the sorted GFP, DP, YFP, and DN cells using an RNeasy Mini Kit (Qiagen) following the standard protocol. Total RNA was processed using SMART-seq v4 Ultra Low Input RNA Kit (Clonetech) following manufacturer's instructions. cDNA was amplified using 11 cycles and eluted in 12 μL. 5 μL of resulting cDNA was processed using a NexteraXT kit (Illumina) following manufacturer's instructions.
GEO Accession
GSM4088948