Illumina NovaSeq 6000 paired end sequencing; RNA-seq to investigate the adult mouse Muller glia transcriptome
RNA-seq to investigate the adult mouse Muller glia transcriptome
Retinas were dissected from adult mice and dissociated using the Papain Dissociation Kit (Worthington). Retinas were dissociated into single cell suspensions and sorted into two fractions: a Muller glia-enriched fraction on the basis of Muller glia-specific tdTomato expression and a second fraction containing the remaining retinal cells that were tdTomato-negative. Both fractions were sequenced. Total RNA was isolated using TRIzol reagent (Invitrogen). RNA molecules were captured using poly-A selection. Libraries were prepared using the Clontech SMARTer low input RNA library kit (Takara).
Experimental Factor: phenotype
tdTomato positive