GSM2601333: Sox9CreErt2:TdTomato collection 2 TdTomato-; Mus musculus; RNA-Seq
Femoral diaphyses were digested in a liberase solution (100ug/mL in PBS) for 2 hours in a fast shaking rocker at 37 oC, 100 rpm to obtain diaphyseal periosteum cells. Digested cells were filtered and single cell suspensions were subjected to FACS sorting to collect Td-Tomato positive and negative cells. About 10,000 Td-Tomato positive cells and 10,000 Td-Tomato negative control cells were isolated after two collections from 18 femora (9 mice). Total RNA was extracted from each cell population separately with Qiagen RNAasy micro kit with DNaseI digestion according to the manufacturer’s instructions. Duplicated experimental RNA sequencing libraries were made with a SMARTer Universal Low Input RNA Kit
GEO Accession
GSM2601333