NextSeq 500 sequencing; Single-cell RNA seq of mouse proximal small intestine at E16.5
Single-cell RNA seq of mouse proximal small intestine at E16.5
Cells were isolated from a pool of six mouse proximal small intestines (1cm of length each) at E16.5 using collagenase treatment and cell sorting as described in the previous section. 20000 DAPInegCD31negCD45negEpCAMpos cells were processed using 10x Genomics Chromium protocols.  RNA was converted into cDNA and amplified as part of the 10x v2 chemistry. Libraries were constructed using the 10x v2 chemistry.
Experimental Factor: individual
mixed pool of 6 fetuses