16S rRNA sequence: human dental biofilm
gDNA extrated using commercial kit (Master Pure DNA Purification Kit, Epicentre, Madison, WI, USA) and stored in -80C. Library preparation protocol consisted of two main amplification steps, one to amplify and tag 16S rRNA variable region V4-V5 (primers Bakt_341F and Bakt_805R), and a second to add final Illumina adapters.