GSM2441027: Nr1d1_ko_CT12_rep2; Mus musculus; RNA-Seq
Livers of all mice were quickly dissected and snap-frozen in liquid nitrogen and stored at −80 °C. Total RNA was extracted from each liver sample using Trizol reagent. For genomic DNA extraction, cells were first lysed in cold lysis buffer (10mMTris HCl, 10mMNaCl, 0.2% NP-40, 1×protease inhibitor) for 15 min on ice. After being washed twice, cell nuclei were re-suspended in Buffer 2.1 (New England Biolabs) including 0.1% SDS and were incubated for 10 min at 65°C. 1% (final concentration) of Triton X-100 was added to quench SDS and centri- fuged to remove SDS and Triton. The RNA samples  were converted into Illumina sequencing libraries by using Illumina TruSeq RNA Sample Prep Kit v2 as per manufacturer's protocol.
GEO Accession
GSM2441027