GSM1163081: RNA_CP_Rep_2_JW-H6B; Mus musculus; RNA-Seq
RNA was extracted using TRIzol reagent according to the manufacturer's instructions. 10 ug RNA was used for library construction according to Illumina RNA-seq library kit with minor modifications.  Briefly, mRNA was isolated using Dynabeads mRNA Purification Kit (Invitrogen) followed by fragmentation (Ambion) and ethanol precipitation. First and second strand synthesis were performed followed by end repair, A-tailing, paired end adaptor ligation and size selection on a Beckman Coulter SPRI TE nucleic acid extractor. 200-400 bp dsDNA was enriched by 15 cycles of PCR with Phusion High-Fidelity DNA Polymerase (NEB) followed by gel purification of 250 bp fragments from the amplified material. Amplified libraries were sequenced on an Illumina GAIIx sequencer.
GEO Accession
GSM1163081