GSM3883383: TwoBound; Mus musculus; MeDIP-Seq
Genomic DNA was phenol-chlorofom extracted. Illumina libraries were prepared before the pull-down using 1-3 micrograms of sonicated genomic DNA.  Libraries were prepared using a 'with-bead' procedure (Fisher et al. 2011) or with the TruSeq DNA sample preparation kit (Illumina) following manufacturer's instructions. Adaptor modified genomic DNA was then immunoprecipitated following (Weber et al. 2007).  Input and pull-down material was whole genome amplified as previously described (Schmidt et al. 2009) except that samples were amplified with 10 PCR cycles, ran on 2% EX agarose gels (LifeTechnologies), size selection of 300 to 500bp fragments with the MinElute gel extraction kit (Qiagen), further amplified with 7 PCR cycles and purified with AMPURE XP beads.  This procedure was also done for amplification with TruSeq reagents. Libraries were qualified and quantified by Bioanalyzer and submitted for sequencing by the CI Genomics core facility. hmeDIP-seq
GEO Accession
GSM3883383