Complete genome sequence of chikungunya virus:Bangladesh 2017
Viral RNA was extracted and DNase treated to remove host and potentially contaminating microbial DNA (Heat & Run Kit, ArcticZymes, Scientifix, South Yarra, Australia). WGS (Illumina, San Diego, CA). cDNA libraries were prepared using the Nextera XT kit for cDNA library construction followed by paired-end (2  151 nt) sequencing using the V2 mid-output kit on a NextSeq 500 machine.