GSM2178343: L_japonica_Green_floral_bud; Lonicera japonica; RNA-Seq
The frozen tissues from L. japonica were powdered using a Multi beads shocker (Yasui Kikai, Japan), and were used for subsequent extraction of total RNA using RNeasy Plant Mini Kit (Qiagen, USA) according to manufacturer’s instructions. RNA quality was assessed using Agilent Bioanalyzer 2100 (Agilent Technology, USA), and RNA samples with RNA integrity number (RIN) above 8 were used for cDNA library preparation. mRNA for each samples were isolated from the total RNA by using beads with oligo (dT), and were added with fragmentation buffer to shear mRNA into short fragments, which were then used as template for the synthesis of first strand of cDNA using random hexamer primers. cDNA library for Illumina sequencing was prepared using SureSelect Strand specific RNA library kit (Agilent Technology, USA) according to manufacturer’s instructions.
GEO Accession
GSM2178343