16S rDNA of eukaryotic community: drinking water treatment plant
18S rDNA gene fragments were amplified using forward primer 1391f5'-GTACACACCGCCCGTC-3' and reverse primer EukBr5'-TGATCCTTCTGCAGGTTCACCTAC-3'. The PCR profile was as follows:94  for 3 min; 35 cycles of 94  for 45 s, 57  for 60 s, and 72  for 90 s; a final extension at 72  for 10 min. And all of the procedures were conducted at Majorbio Bio-Pharm Technology Co., Ltd., Shanghai, China.