Bacterial community composition of composite soil sample from long-term maize monoculture field layer 'A' collected on June 2017
A part of the 16S rRNA gene was amplified with fusion primers containing the Bacteria-specific sequences Bakt_341F (5-CCT ACG GGN GGC WGC AG-3) and modified Bakt_805R (5-GAC TAC NVG GGT ATC TAA TCC-3) (Herlemann et al. 2011), respectively, and Fluidigm CS1 and CS2 universal sequences at their 5' ends.