pigs_jiangsu_3
The procedure of library preparation for GGRS approach was improved based on RAD-seq and GBS to adapt genotyping for outbred populations.Briefly, high molecular weight genomic DNA samples were extracted from ear tissue, digested with AvaII and then ligated with a unique adapter barcode. Next, the samples were pooled and enriched to construct a sequencing library. Finally, the sequence libraries (fragments ranging from 300 to 400 bp, including the adapter barcode sequence) were sequenced on an Illumina Hiseq2500 instrument.