GSM3639564: Bulk_wing_disc; Drosophila melanogaster; RNA-Seq
Larvae were dissected in Schneider's medium in batches of 5 animals (to prevent hypoxia) and transferred into a tube containing Schneider's medium on ice for a maximum time of 30 minutes. To isolate single cells TrypLE (10x) was added and the tissue incubated for 15 minutes in a water bath at 37°C , with gentle mixing every 5 minutes. Schneider's medium was then added to the loosened tissue pellets, followed by gentle mechanical dissociation using a P1000 pipette. The cell suspension was then passed through a 10 μM cell strainer to remove undigested tissue and cell clumps. Libraries were prepared according to Illumina's instructions accompanying the TruSeq Stranded mRNA Sample Preparation Kit.
GEO Accession
GSM3639564