paired-end sequencing raw reads from pooled intact and regenerating Dlac
Total RNA from pooled intact and day 1- 6 regenerating Dlac was isolated using the TRIzol (Invitrogen) standard protocol, quality controlled on a Bioanalyzer (Agilent), poly-A selected using Sera-Mag Oligo(dT) beads (Thermo Scientific), library prepared using NEBNext mRNA Library Prep Reagent Set for Illumina (NEB), and processed for Illumina Sequencing (HiSeq 2000).