Salmonella enterica subsp. enterica serovar Rissen Genome with antibiotic-multinresistance profile including mcr-1 mediated resistance to Colistin sequencing and assembly
For whole-genome sequencing, the strain was grown overnight at 37 C in 3 ml of Luria-Bertani broth (Mast Diagnostica GmbH, Reinfeld, Germany). DNA sequencing libraries were constructed using the Nextera FLEX DNA preparation kit in accordance with the manufacturers instructions. Paired-end sequencing was performed on the Illumina MiSeq platform (Illumina Inc., San Diego, CA, USA) using a 300-cycle MiSeq reagent kit. In total, 482,366 reads were generated. A quality check was performed with FastQC v0.11.7 (available at http://www.bioinformatics.babraham.ac.uk/projects/fastqc/)