GSM4091270: WT3; Triticum aestivum; RNA-Seq
Total RNA was extracted, purified, quantified and sequenced as described previously (Buhrow et al., 2016). In total, 35 samples were collected; total RNA, containing both the plant and  pathogen RNA present in the samples, was extracted using the TriReagent (Molecular Research Center Inc.) followed by a treatment with DNase I (RNase-Free DNase set, Qiagen) onto columns (RNeasy Mini kit, Quiagen) according to instructions provided by the manufacturer.  RNA was processed for deep paired-end RNA sequencing using Illumina HiSeq 2500 by the  National Research Council Canada sequencing service Libraries were prepared according to Illumina's instructions, using the TruSeq RNA v2 LT kit and sequenced on the Genome Analyzer following the manufacturer's protocols (https://www.illumina.com/documents/products/datasheets/datasheet_hiseq2500.pdf).
GEO Accession
GSM4091270