GSM3958062: 40B: SJSA-1_H3K29Ac_C; Homo sapiens; ChIP-Seq
U2-OS and SJSA-1 cells were cultured at 37 °C in 5% CO2, and were cross-linked with 1% formaldehyde at room temperature for 10 min followed by neutralization with glycine. Cells were resuspended, lysed in lysis buffer, and sonicated on ice to shear most DNA to 200-750 bp. Libraries were generated for ChIP samples following the NEBNext® Ultra™DNA Library Prep Kit for Illumina protocol.
GEO Accession
GSM3958062