GSM3108742: AD-Phe MX04 Neg 1; Mus musculus; RNA-Seq
Sorted microglia were collected in 500 μl RLT Buffer (Qiagen) and total RNA was isolated using the RNeasy Mini Plus Kit (Qiagen). 1 ng of total RNA was preamplified with the SMARter Ultra Low Input kit V2 (Clontech, Mountain View, CA). Libraries were constructed using the NexteraXT library kit (Illumina, San Diego, CA) and sequenced on the Illumina HiSeq2500 to generate approximately 20 million 100-bp single end-reads per sample.
GEO Accession
GSM3108742