Illumina HiSeq 2500 sequencing; Timecourse single-cell RNAseq of whole mouse embryos harvested between days 6.5 and 8.5 of development.
Timecourse single-cell RNAseq of whole mouse embryos harvested between days 6.5 and 8.5 of development.
Embryos from the same stage were pooled, and single-cell suspensions were prepared by incubating the embryos with TrypLE Express dissociation reagent (Life Technologies) at 37 °C for 7 min and quenching with heat inactivated serum.  Steps were performed according to manufacturers' instructions (10X Genomics, v1 chemistry). Steps were performed according to manufacturers' instructions (10X Genomics, v1 chemistry). Specific kits used were: Chromium Single Cell 3' Library Kit P/N 120230, Chromium Single Cell 3' Gel Bead Kit P/N 120231, Chromium Single Cell 3' Chip Kit P/N 120232.
Experimental Factor: developmental stage
mixed