GSM3400981: RNAseq_In_vitro_AntimycinA_1; Mus musculus; RNA-Seq
NEB kit Cells were lysed with RLT Buffer from the Qiagen RNeasy Plus Mini kit (Qiagen). Cell lysates were stored at -80°C until RNA was extracted. RNA isolations were performed using Qiagen RNeasy Plus Micro/Mini kit (Qiagen) (Extended Data Fig. 5) or the AllPrep DNA/RNA Micro Kit (Qiagen) (Figure 3 and 4) following the manufacturer's protocol with an additional on-column DNase treatment using the RNase-Free DNase Set (Qiagen). RNA quality and quantity were measured using Agilent 4200 Tapestation using the high-sensitivity RNA ScreenTape System.  (Agilent Technologies). mRNA libraries were prepared using the SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio USA, Inc) +Nextera XT DNA sample preparation kit (Illumina Inc) (Extended Data Fig. 5) or the NEBNext Ultra I RNA Library Prep Kit for Illumina with poly (A) mRNA selection (NEB Inc) (Figure 3,4).
GEO Accession
GSM3400981