Multiplexed libraries were prepared using Illumina Genomic DNA Sample preparation protocol with NEXTflexTM DNA Sequencing Kit (Bioo Scientific) and NEXTflex-96ť DNA Barcodes (Bioo Scientific). Size selection and PCR clean-up were performed with Agencourt AMPure XP Beads (Beckman Coulter). After PCR enrichment, libraries were validated with Fragment Analyzerť Automated CE System (Advanced Analytical) and pooled in equimolar concentration for 96X-multiplex.  Libraries were sequenced on Illumina HiSeqť 2500 Analyzers using manufacturer's standard cluster generation and sequencing protocols in 125 bp PE mode.