16S amplicon seq of Delia radicum pupae
40 pupa were collected by flooding eggbox 30-12-2017 with tap water, and running top liquid through a sieve. Prior to DNA extraction, all pupa were washed in 5 mL sterile MQ water, then in 5 mL 70% EtOH, then in 5.5-7.5% active chlorine bleach, then rised in 5 mL MQ again. Subsequently, all whole insects were homogenized by mortar and pestile in liquid nitrogen.  The dust was used in sample PUPA 5