RNA-seq of T. aotearoense SCUT27/ldh-G58: resistant to high sugar medium
The evolved SCUT/ldh-G58 was incubated with 120 g/L of carbon sources (glucose:xylose = 2:1, g:g). When the cell density of OD600 reached 0.8¨C1.0 (in exponential phase), 1.0 OD cell was harvested via centrifugation (12000 rpm ¡Á 2 min) at 4 ¡ãC. Total RNA was extracted using RNAprep pure kit (for cell/bacteria, TIANGEN, Beijing, China) according to the manufacturer¡¯s instructions.