GSM1446037: Infected small intestine repeat 1; Gallus gallus; miRNA-Seq
Small intestine was removed, flash frozen by dry ice, and RNA was isolated using Trizol reagent The total RNA was ligated  5′ and 3′ RNA adapters  by T4 RNA ligase, followed by reverse transcription and PCR product purification.The 145-160bp fraction was obtained through PAGE electrophoresis to quantified on the Qubit (Invitrogen), then were diluted to 10 nM to finish cluster generation
GEO Accession
GSM1446037