GSM3262228: KWZ15; Homo sapiens; RNA-Seq
RNA was isolated using the NucleoSpin kit (Macherey Nagel) or RNeasy Microkit (Qiagen). For RNA-seq analysis, 500ng of total RNA was fragmented, followed by reverse transcription and second strand cDNA synthesis. The double stranded cDNA was subjected to end repair, A base addition, adapter ligation and PCR amplification to create libraries. Libraries were evaluated by Qubit and TapeStation. Sequencing libraries were constructed with barcodes to allow multiplexing of multiple samples over 3 lanes.
GEO Accession
GSM3262228