GSM3336940: mrna_3; Homo sapiens; RNA-Seq
Total RNA was extracted from NPC specimens and normal nasopharyngeal mucosal specimens using TRIzol reagent (Invitrogen; Carlsbad, CA, USA). The RNA concentration was measured with a NanoDrop2000 Spectrophotometer (NanoDrop Technologies; Wilmington, DE, USA), and the integrity of purified RNA was determined using Agilent 2100 Bioanalyzer (Agilent Technologies; Palo Alto, CA USA). Then RNA was treated with Ribo-ZeroTM Magnetic Kit(Human) (Epicentre) to deplete rRNA. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM3336940