Illumina HiSeq 2000 paired end sequencing; RNA-seq from knee and hip osteoarthritis cartilage
RNA-seq from knee and hip osteoarthritis cartilage
Preserved and lesioned cartilage samples from the same donor were obtained from the RAAK study consisting of OA patients that underwent a joint replacement surgery due to end-stage disease.collection (within 2 hours following surgery) tissue was washed extensively with phosphate buffered saline (PBS) to decrease the risk of contamination by blood. Cartilage was classified macroscopically and collected separately from OA affected and preserved regions around the weight-bearing area of the joint. Total RNA from articular cartilage was isolated using Qiagen RNeasy Mini Kit (Qiagen, GmbH, Hilden, Germany).  Paired-end 2×100 bp RNA library sequencing (Illumina TruSeq RNA Library Prep Kit) were constructed. Strand specific RNA-Seq libraries were generated and yielded a mean of 20 million reads per sample. Stranded TruSeq kit
Experimental Factor: sampling site
hip
Experimental Factor: disease staging
preserved osteoarthritis