Phytophthora capsici germinating cysts with germ tubes on a cellophane membrane placed on a Nicotiana benthamiana
We used a cellophane membrane placed on a host leaf as a surface for the induction of cyst germination in order to carry out molecular investigation of the early infection events by P. capsici but without the involvement of plant biomass into later processing. RNA sample from Phytophthora capsici germinating cysts were deeply sequenced using Illumina RNA-Seq. About 12 million (M) reads with ~94 % Q20 bases (those with a base quality greater than 20) were selected as high quality reads for the library.