RNA-seq of Anatolikos japonicus: Hepatopancreas, gill and muscle
RNA from four tissues/organs (muscle, gill, hepatopancreas and gonad) were extracted separately using TRIzol reagent (Invitrogen) and purified with PureLink RNA Mini kit (Ambion/Life Technologies, Thermo Fisher Scientific, Waltham, Massachusetts, USA). These were then pooled equally for library preparation. cDNA libraries were prepared using the TruSeq RNA Sample Preparation Kit following the manufacturers instructions. Preparation of libraries, quality assessment and paired-end 2 x 150 bp Illumina sequencing were carried out by the commercial company Genomics BioSci & Tech Co. Ltd. (Taipei, Taiwan).