GSM2176805: NC-rMSC; Rattus norvegicus; RNA-Seq
RNA was extracted with trizol-based techniques. The quantity of RNA was determined with an ultraviolet spectrometer and electrophoresis in a denaturing formaldehyde agarose gel. Subsequently we used oligo(dT) beads to isolate mRNA, and added the fragmentation buffer to disrupt the mRNA. The first-strand cDNA were synthesized using the random hexamer primer. The second-strand cDNA were synthesized using the first-strand cDNA as the template. The templates were amplified with PCR, purified with the QiaQuick PCR extraction kit after the addition of a poly(A) tail connected with sequencing adapters. The sizes of the fragments were confirmed with an agarose gel electrophoresis and the sequences were determined with 1G Illumina GA platform.
GEO Accession
GSM2176805