Iris fulva floral/leaf transcriptome
Total RNA from each sample was quantified by fluorimetry and pooled for equimolar representation.  One microgram of the pool was used to prepare a library optimized for Roche/454 Titanium sequencing that included partial normalization with double-stranded nuclease and PCR suppression of misligated products (Carter et al., in press; prepublication details are available by request to K. Mockaitis, Indiana University).  Reads were cleaned of adaptor sequences using http://sourceforge.net/projects/estclean.