GSM3038719: SIC_InfectedMO_liveCA_t120_S71; Candida albicans; Mus musculus; RNA-Seq
Subpopulation samples were harvested, collected via FACs, flash frozen on dry ice, and RNA was extracted after bead mill lysis in tubes containg RLT and BME and the Qiagen Rneasy mini kit. Single, C. albicans infected libraries were sorted into wells of a 96 well plate containining RLT and BME, and frozen at -80 utnil cDNA synthesis. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols Smart-seq2 was used to create cDNA, Nextera XT kits were used to make libraries;subpopulation and single infect cells were sequenced on Illumina's Nextseq. Candida only samples were sequenced on Illumina's Miseq
GEO Accession
GSM3038719