GSM4454569: double deletion CBS/CSE(2) [MBE2_2]; Drosophila melanogaster; RNA-Seq
RNA was isolated from 5 days male and female flies: control line-58492, CBS lines-CBS5 and CBS8, CSE1, MST15, with double deletion CBS5 /CSE1, and CBS8 /CSE1 using RNAzol RT according to the manufacturer's protocol. RNA quality was assessed using an Agilent 2100 Bioanalyzer instrument (Agilent Technologies, USA) using the Agilent RNA 6000 nano Kit (Agilent Technologies). The concentration of RNA samples was evaluated on a Qubit 2.0 Thermo fluorimeter (Fisher Scientific) Libraries were prepared from 1000 ng of total RNA using the NEBNext® Ultra ™ II Directional RNA Library Prep Kit for Illumina (NEB, UK) according to the manufacturer's protocol.
GEO Accession
GSM4454569