GSM3109304: eWAT macrophage, Cd9+, rep2; Mus musculus; ATAC-seq
ATAC-seq was performed using a modified protocol based on the technique published by Buenrostro et al. Briefly, sort-purified cell populations were washed in PBS and lysed with lysis buffer (1 M Tris•Cl, pH 7.4, 5 M NaCl, 1 M MgCl2; 10% NP-40 in nuclease-free H2O) and gentle agitation prior to treatment with Tn5 transposase. DNA was purified (Qiagen MinElute Reaction Cleanup Kit), and amplified with NEBNext High-Fidelity 2x PCR Master Mix (New England Biolabs) using barcoded primers. DNA library was purified with AMPure XP beads, and library quality was assayed via Qubit and Agilent High Sensitivity DNA Bioanalysis chip. Libraries were sequenced via 40 bp paired-end sequencing using the Illumina NextSeq 500 platform.
GEO Accession
GSM3109304