"H3K4me3 PLAC-seq for patient HMG061, NS002, isolated Neuron": Sample NS002
"Neuron nuclei were isolated from brain tissue crosslinked with paraformaldehyde by sorting using NEUN antibody and snap-frozen in liquid nitrogen. Nuclei were thawed, lysed, and chromatin was digested with MboI at 37oC for 2 hours. Fragmented ends were labled with biotin and then ligated. Samples were chromatin immunoprecipitated using Dynabeads coated with H3K4me3 antibody.  Samples were treated with RNase A and Proteinase K and reverse cross-linked at 65oC. Libraies were prepared using QIAseq Ultralow Input Library Kit. Libraries were PCR-amplified, size-selected with 0.5X and 1X SPRI Cleanup and paired-end sequenced."