Dropout CRISPRa screen with secondary pool using dCas9-VP64 in MelJuSo, Replicate B
MelJuSo cells expressing dCas9-VP64 were infected with the CRISPRa secondary pool(from ORF and Calabrese screens), passaged in standard growth conditions, Day21 time point was collected, genomic DNA was PCR amplified and sequenced