GSM1979166: MOI20 Ad-AldoA, one miR-122 6mer site, rep 2; Mus musculus; RNA-Seq
RNA was extracted using Trizol according to the manufacturer's instructions and the RNA treated with DNase prior to library preparation. For RNA-seq, total RNA was treated with the DNA-free Kit (Life Technologies), cleaned up with an RNAeasy MinElute Cleanup Kit (Qiagen) and the quality controlled with an Agilent 2100 Bioanalyzer. Library preparation and sequencing were performed by Fasteris SA (Switzerland) or by the Functional Genomics Centre Zürich (FGCZ), using an Illumina HiSeq 2500 machine. For small RNA-seq total RNA was size fractionated (18–35 nt), followed by ligation of 3’ preadenylated adapter (AppNNNNAGATCGGAAGAGCACACGTCTddC) and 5’ adapter (Truseq small RNA 5’ adapter RA5 + 8Ns, GUUCAGAGUUCUACAGUCCGACGAUCNNNNNNNN), reverse transcription, PCR amplification, and Solexa sequencing. The 8 and 4 random nucleotides (Ns) on the 5' and 3' ends, respectively, were added to minimize the effect of ligation bias during the quantification step.
GEO Accession
GSM1979166