GSM4022277: WM164 parent Input-2; Homo sapiens; ChIP-Seq
Chromatin immune precipitation was performed using the Active Motif ChIP-IT® Express Chromatin Immunoprecipitation Kits (53009). WM164 parent, IDTC colonies and dabrafenib resistant cells were fixed using 4% paraformaldehyde for 10 minutes followed by chromatin preparation according to the manufacturer's protocol. 15 microgram DNA containing chromatin was used as input per reaction. The libraries were prepared using ThruPLEX DNA-seq kit (Takara Bio) according to the manufacturer's protocol. All libraries were uniquely indexed and pooled for single-read sequencing across 8 lanes of HiSeq 2500 High Output, V4 chemistry (Illumina, Inc.), generating 50bp reads.
GEO Accession
GSM4022277