Bisulfite-Seq of potato tissue: immature small tuber (transection diameter < 1 cm), the third replicate.
Genomic DNA was extracted using the CTAB method and fragmented by sonication to 200~300 bp prior to library construction. The barcoded DNA was treated twice with bisulfite using EZ DNA Methylation-GoldTM Kit (Zymo Research) according to manufacturer's instructions. The libraries were sequenced on a Novaseq platform.