DNAseq of berry bacteria
the V3-V4 region of the 16S rRNA gene was amplified for bacteria.  In order to conduct multiplexing of samples within a single sequencing run and to assign sequences to samples in bioinformatics analysis, a specific tag was added to both forward and reverse primers. PCR products were purified through Agencourt AMPure XP beads (Beckman Coulter, Indianapolis, USA) according to manufacturers instructions and combined in equal molar concentration to generate a library pool. 16S rRNA gene libraries were sequenced at CIBIO (Center of Integrative Biology)  University of Trento (Trento, Italy).