Sequencing of chromosome specific DNA pools (ASA)
We sequenced and analyzed chromosome-specific DNA pools derived by flow sorting chromosome suspensions of A. carolinensis and A. sagrei to study the genetic content of microchromosomes and to reconstruct possible events that could have led to the sex chromosomes of A. sagrei. Furthermore, by sequencing microchromosomes of A. carolinensis we assigned 189 Mbp to specific microchromosomes in the unfinished reference AnoCar2.0 assembly. Reamplification of DNA from flow sorted chromosomes was carried out by Degenerate oligonucleotide-primed PCR (DOP-PCR)