GSM4295143: Foreskin fibroblasts, ATAC-seq deep senescence rep1; Homo sapiens; ATAC-seq
~25,000 cells were harvested and then lysed in cold lysis buffer (10 mM Tris-HCl, 10 mM NaCl, 3 mM MgCl2, 0.5% Igepal CA-630) for 10 min. Centrifuge at 500× g for 10 min at 4℃. Discard the supernatant and resuspend pellet in a 50 ul transposition mix (TruePrep™ DNA Library Prep Kit V2 for Illumina, TD501). Incubate the transposition system at 37℃ for 1 hour and purify transposed DNA using Zymo DNA Clean & Concentrator™-5 kit. Finally ATAC-seq libraries were PCR amplified for 10 cycles and purified using Zymo DNA Clean & Concentrator™-5 kit. Illumina HiSeq 2500
GEO Accession
GSM4295143