GSM2179739: SNS_F5; Leishmania major strain Friedlin; OTHER
Nuclei were lysed and DNA was purified by phenol/cloroform extraction. DNA was denaturated (boiling) and size fractionated through a sucrose gradient centrifugation and fractions containing the sizes of interest (F4 and F5) were digested three times with lambda-exonucleasa. Finally, ssDNA was converted into dsDNA prior library preparation Each protocol is detailed in the SAMPLES section DNA libraries were prepared for sequencing using Illumina standard protocols
GEO Accession
GSM2179739