RNA-Seq of Crassostrea gigas Larvae : post Bacteria Challenge at different times
The cleaved RNA fragments were transcribed into first strand cDNA using a random hexamer primer and M-MuLV Reverse (RNase H). Second strand cDNA was subsequently synthesized by DNA polymerase I and RNase H.  NEBNext Adaptor was added to ligate to the end-repaired, dA-tailed DNA to prepare for hybridization, then adaptor-ligated fragments were purified with the AMPure XP system (Beckman Coulter, Beverly, USA). After PCR amplification, DNA fragments in the range of 200 to 250 bp were obtained for the following procedures. Finally, the libraries were sequenced on an Illumina HiSeq 2500 platform and 127bp paired-end reads were generated.