S10
Bar-coded small RNA sequencing libraries were generated using the Ion Total RNA-Seq Kit v2 and the Ion Xpress RNA-Seq bar coding kit (Thermo Fisher Scientific). Size distribution and yield of the resulting barcoded libraries were assessed using the 2200 TapeStation System with Agilent D1K ScreenTapes (Agilent Technologies). Libraries were prepared for sequencing on the Ion Chef System (Thermo Fisher Scientific). Sequencing was performed on an Ion Proton System using Ion PI v3 chips (Thermo Fisher Scientific).