Illumina HiSeq 2500 paired end sequencing; RNA-seq of whole blood samples from patients with visceral leishmaniasis.
RNA-seq of whole blood samples from patients with visceral leishmaniasis.
blood samples from healthy donors and patients were collected using BD Vacutainer and PAXgene Blood RNA tubes. Samples tagged with \"D180\" were collected from the same patients diagnosed with visceral leishmaniasis, but 180 days later after being treated and are therefore considered cured at that time. Total RNA samples were purified with the PAXgene Blood RNA Kit and GLOBINclear-Human kit. Libraries were prepared using TruSeq™ Stranded RNA Sample Preparation kit with Poli(A)+ selection.
Experimental Factor: disease
normal
Experimental Factor: clinical history
healthy, no previous infection