GSM4708372: HydroL10_PlusATC_Rep3; synthetic construct; OTHER
Before and after selection procedures, plasmid DNA was extracted using enzymatic yeast cell wall degradation in combination with standard extraction methods. The gRNA barcodes amplified from the one-copy-per-cell CEN-plasmids. Illumina sequencing was performed in paired-end and 75-100 base pairs read length on NextSeq500 machines with 15% PhiX spike-in. DNA barcode libraries were generated by introducing standard Illumina P5 and P7 adapters and inline sample barcodes by PCR. Library preparation is otherwise performed according to standard Illumina protocols
GEO Accession
GSM4708372