RNA-Seq of Genome Replication Defective  (C1216) HSV-11
MRC5 cells were infected with wild type  (KOS) and genome replication defective (UL30 C1216) HSV-1 at an MOI of 10  PFU/cell. Samples were harvested at 2,  4, 6, 8, 12, and 24 hpi. RNA was isolated and a poly(A) mRNA selection was  performed. Illumina sequencing was carried out at Tufts University Core  Facility using HiSeq 2500 to generate 50 bp single-end reads.