Illumina HiSeq 2000 sequencing; RNAseq profiling of a lpp0148 mutant compared to its isogenic parent Legionella pneumophila strain Paris
RNAseq profiling of a lpp0148 mutant compared to its isogenic parent Legionella pneumophila strain Paris
Bacterial cultures were centrifuged at 5000g for 5 minutes. Pellets were stored at -80C Legionella pneumophila strains in this analysis were grown in AYE medium until they reach an optical density of 0.8 to 0.9 RNA samples were treated with DNase I (Zymo Research) and further purified on silica-based column (Zymo Research) RNA were extracted from frozen pellet with 1 mL of Trizol (Invitrogen) warmed at 65C. RNA extraction was performed as recommended by the manufacturer Libraries for sequencing were constructed with the ScriptSeq v2 kit (Epicentre)
Experimental Factor: wild type genotype
genotype