20%_Shaker_1
RNA was extracted using RNeasy plus (Qiagen) followed by quantification using Qubit RNA High Sensitivity assay and quality assessed using a Fragment Analyzer RNA Kit. Four groups were studied, 20% O2 static (n=3), 20% O2 shaking (n=2), 1% O2 static (n=2), 1% O2 shaking (n=2). RNA was sent to Novogene for library prep and sequencing. In short, PolyA capture RNA libraries were generated and sequenced for paired end 150 cycle reads.