RNAseq of stamens and carpels from  Mimulus guttatus IM62, M. nasutus SF5, and sterile (STE) and fertile (FER)  SF5-IM62 introgression hybrids
For this study, we generated 24 RNAseq libraries representing three  biological replicates each of two tissue types (stamens and carpels) from  four genotypes that vary at hms1 and hms2 (M. guttatus IM62, M. nasutus SF5,  and fertile (FER) and sterile (STE) SF5-IM62 introgression progeny. To  collect sufficient tissue for each biological replicate, we dissected eight  to 24 pre-anthesis floral buds and transferred carpel and stamen tissue into  separate 1.5 mL microcentrifuge tubes partially submerged in liquid nitrogen.  We extracted RNA using a QuickRNA Miniprep Kit (Zymo Research, Irvine, CA,  USA) then measured RNA concentration using a Qubit RNA BR (Broad-Range) Assay  Kit and a Qubit 2.0 Fluorometer (Thermo Fisher Scientific Inc., Waltham, MA,  USA). We shipped RNA samples overnight on dry ice to the Duke Center for  Genomic and Computational Biology (Durham, NC, USA), where the Sequencing and  Genomic Technologies core checked RNA quality using an Bioanalyzer 2100  (Agilent Technologies, Santa Clara, CA, USA), constructed sequencing  libraries using a KAPA Stranded mRNA-Seq Kit (F. Hoffmann-L Roche, Basel,  Switzerland), and sequenced all 24 libraries on a single lane of HiSeq 4000  (Illumina, Inc. San Diego, CA, USA), producing 50bp single-end reads.