GSM4201207: Jeko-1-Sen [ChIP-seq]; Homo sapiens; ChIP-Seq
ChIP-seq was performed according to established protocols, with minor modifications. 50 million cells were used for ChIP-seq of H3K27ac. DNA was isolated with phenol chloroform extraction and ethanol precipitation Libraries for Illumina sequencing were prepared using ThruPLEX DNA-seq Kit (Rubicon) using 50 ng of DNA or less and amplifying according to manufacturer instructions. Amplified libraries were size-selected first using AMPure beads (Agencourt AMPure XP) and subsequently, using a 2% gel cassette in the Pippin Prep (SAGE Sciences) to capture fragments of 200-700 bp. Libraries were quantified by qPCR using the KAPA Biosystems library quantification kit, multiplexed with equimolar DNA content, and sequenced on an Illumina NextSeq 500 (single end 75 bp reads).
GEO Accession
GSM4201207