GSM4089269: MDA-MB-231_rep3_smallRNAseq; Homo sapiens; ncRNA-Seq
Small RNA-seq: Total RNA was directly adaptor-ligated, reverse-transcribed and PCR-amplified for 12 cycles. Cells were grown in 60mm dishes for 48h. Total RNA from HeLa, HEK293, HCT116, MDA-MB-231 and fibroblast BJ/hTERT was extracted using the miRNeasy Mini kit. Independent replicates where grown and RNA was extracted on different days. Upon applying either the hydro-tRNAseq or small RNA-seq protocol, the resulting cDNA was purified using a QIAQuick PCR Purification Kit. The adaptor ligated to the 3' end of the RNA is the standard TruSeq adaptor: 5'-AGATCGGAAGAGCACACGTCT-3'. The adaptor ligated to the 5' end is the TruSeq smallRNA adaptor: 5'-GTTCAGAGTTCTACAGTCCGACGATC-3'.
GEO Accession
GSM4089269