G.candidum 3c RNA-seq Ion PGM
Strain was cultivated for 7 days in medium containing: KH2PO4, 1 g/L; NaNO3, 1,5 g/L; (NH4)2SO4, 1,5 g/L; MgSO4  7H2O, 0,5 g/L; wheat bran, 40 g/L and filter paper 10 g/L. Culture samples from days 2, 3 and 7 were used for RNA extraction. We used Quick-RNA(TM) MicroPrep (Zymo Research, USA) for total RNA extraction and GenEluteTM mRNA Miniprep Kit (Sigma-Aldrich, USA) for mRNA purification. We used 1,8 ng of pooled mRNA for library preparation using Ion Total RNA-Seq Kit v2. Library was sequenced on IonTorrent PGM using Ion PGM  Sequencing 400 Kit and Ion 318  Chip v2 (Thermo Fisher Scientific, USA).