GSM2087545: HSC 42 rep3 w/ spike; Mus musculus; RNA-Seq
Total RNA was isolated by RNeasy mini kit (Qiagen) and Pan et al. 2013 protocol for bulk RNA-seq ;  C1 Fluidigm for mRNA seq protocol for single-cell RNA-seq. For single cell RNA-seq, we also used ArrayControl RNA spikes (Thermo Fisher Cat# AM1780) and prepared RNA spike mixture  following the instruction. Bulk RNA-seq library preparation was conducted using standard Tru-Seq Illumina kits. Single cell RNA-seq library is constructed by using Nextera XT DNA Library Preparation Kit (96 samples).
GEO Accession
GSM2087545