Metagenomic analysis of fungal biodiversity from rhizospheric soil amended with non-sterilized vermicompost from olive-mill waste by Illumina-based 18S rRNA gene sequencing
The analysis corresponds to 18S rRNA amplicons sequenced with Illumina technology (MiSeq run PE 2x300), using primers 563F and 1132r. DNA template was DNA sample extracted from the rhizosphere of the amended soil by the bead-beating method, following the manufacturer’s instructions for the MoBio UltraClean Soil DNA Isolation kit (MoBio laboratories, Solana Beach, CA, USA).