Whole genome sequencing of influenza experimental H9 trachaeal swab in quail
RNA extractions were performed directly from swab material using the Roche MagNA Pure LC instrument and RNA HP Cells kit. Influenza genome was amplified by MS-RTPCR using an optimized, universal primer set. The libraries were prepared using NexteraXT (Illumina) and loaded at a concentration of 12-14pM on the MiSeq v2,300 cycle kit for a 150x2 paired end sequencing run.