GSM1981225: Md_ED15.0; Mus musculus; RNA-Seq
Total RNA was prepared using the RNeasy micro kit from QIAGEN following lysis with a Precellys homogenizer. RNA integrity was controlled on a Bioanalyzer (Agilent Technologies, a RIN of 10 was reached for all samples used in this study). PolyA+ libraries of the large-scale dataset were prepared with the Truseq V2 kit (Illumina), starting with 150ng total mRNA and reducing the amplification step to only 12 cycles.  Sequencing was done on an Illumina Hi-seq2000sequencer (100 bp paired-end sequences)
GEO Accession
GSM1981225