PacBio subheads
Around 20 micrograms of high quality genomic DNA was sheared using Hydroshear. The Bluepippin was used to select 20Kb double-stranded DNA fragments. Then, DNA fragments were end repaired and ligated with universal hairpin adapters. Subsequent steps were followed as per the manufacture’s instruction to prepare SMRTbell library.  The library was sequenced in PacBio RS SMRT instrument.