GSM2597683: HiC_IMR90_EMPTY_GM_Rep2; Homo sapiens; Hi-C
For RNASeq: cells were lyzed with Trizol and RNA was extracted following manufacturer's instruction. For Hi-C, DNA crossliked, digested and ligated, ligation products were isolated with streptavidine beads, Hi-C experiment were conducted in biological replicates using DpnIII, according to the protocol published by Rao et al, 2014 with slight modifications RNASeq libraries were prepared using TruSeq Stranded mRNA Library Prep Kit set A RS-122-2101 following Illumina's recomandations. Hi-C experiment were conducted in biological replicates using DpnIII, according to the protocol published by Rao et al, 2014 with slight modifications
GEO Accession
GSM2597683