GSM2359752: wt 1h (sRNA-seq); Schizosaccharomyces pombe; ncRNA-Seq
Total RNA was prepared as described above from which sRNAs (less than 200bp long) were size-selected using the RNAeasy Mini kit (Qiagen). Purified sRNA (20-30nt) samples were obtained by gel extraction.  Gel slices were resuspended in 0.4 M sodium acetate (pH 5.2) overnight at 4oC, and sRNA were collected by ethanol precipitation. Sequencing libraries were prepared using the Illumina TruSeq Small RNA Library Preparation kit using 25ng of sRNA.
GEO Accession
GSM2359752