Hybridization capture and targeted enrichment of Cedrela angustifolia
We designed hybridization capture probes to enrich diverse genes from species in the genus Cedrela. We first assembled a draft de novo transcriptome reference for C. odorata, and then identified putatively low-copy genes. Hybridization probes were designed from 10,001 putative low-copy gene models. These gene models were used to design 19,740 custom 100 bp biotinylated RNA hybridization probes (MYbaits, Arbor Biosciences, Ann Arbor, MI, USA), with two RNA probes per gene target tiled end-to-end beginning at the 5 end of the gene target. Captured libraries were multiplexed for sequencing of 96 individuals per lane.