GSM1982090: Super_DAL8.rep3; Cucumis sativus; RNA-Seq
As described above, 11 kinds of corolla samples were collected for RNA-seq analysis. For each sample, 5 individual corollas were ground into powder and mixed in liquid nitrogen (three replicates). Total RNAs were isolated using the RNA extraction kit (Huayueyang, China). RNA was checked on 1% agarose gels to avoid possible degradation and contamination, and was then examined by a Nano Photometer spectrophotometer (IMPLEN, CA, USA) for RNA purity. Qubit RNA Assay Kit in Qubit 2.0 Flurometer (Life Technologies, CA, USA) was used to measure RNA concentration, and RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA) was used to evaluate RNA integrity. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM1982090