GSM2087627: NI_1_smallRNA-Seq; Mus musculus; miRNA-Seq
Small RNAS were purified from iBN cells using the miRNeasy mini kit (Qiagen), and eluted in a final volume of 30µL RNase-free water Total RNA was quantified using a NanoDrop Spectrophotometer ND-1000 and its integrity was assessed using a 2100 Bioanalyzer. Libraries were generated from 1000 ng of total RNA using the TruSeq® Small RNA Sample Prep Kit (Illumina), as per the manufacturer's recommendations. cDNA construct purification has been performed on a Pippin Prep instrument. Final libraries were quantified using the Quant-iT™ PicoGreen® dsDNA Assay Kit and the Kapa Illumina GA with Revised Primers-SYBR Fast Universal kit. Average size fragment was determined using a LaChip GX (PerkinElmer) instrument. Libraries were multiplexed and sequenced on an Illumina HiSeq 2000 SR50.
GEO Accession
GSM2087627