GSM2947856: CD31posCD144pos day 10 no CK replicate 2; Homo sapiens; RNA-Seq
RNA was extracted using the Qiagen RNAeasy micro kit. Indexed total RNA libraries were prepared using 10ng of Total RNA, a 4 minute fragmentation time, and 12 cycles of amplification in the Clontech SMARTer Stranded Total RNA-Seq Kit-Pico input ( Cat No: 635009). Libraries were quantified by qPCR using a Kapa Library Quantification Kit for Illumina sequencing platforms (Kapa Biosystems Inc. Cat No: KK4835). Single end 75bp sequencing was carried out by clustering 1.8 pM of the pooled libraries on a NextSeq 500 sequencer (Illumina inc.)
GEO Accession
GSM2947856