WGS of Escherichia coli EH41
The library was prepared using the Nanopore 1D Ligation sequencing kit SQK-LSK108 (R9 version) with the Native barcoding kit EXP-NBD103 according to the manufacturers instructions, and sequenced on a MinION Mk1B with a SpotON flow cell FLO-MIN107 (R9). Briefly, genomic DNA was fragmented, end-repaired, A-tailed, and ligated with the adaptors. Then, the libraries were normalized and pooled for sequencing.