GSM4023334: Sheep whole blood - T24 - Ad5-FMDV [Ad5-FMDV_T24_rep4]; Ovis aries; RNA-Seq
RNA was extracted from the PAXgene Blood RNA tubes using the PAXgene Blood RNA kit and purified with the RNeasy MinElute Cleanup kit (QIAGEN). The quality of the total RNA was assessed on an Agilent Bioanalyzer 2100, using RNA 6000 pico kit (Agilent Technologies). Directional RNA-Seq Libraries were constructed from 1 µg of total RNA using the TruSeq mRNA Stranded library prep kit (Illumina), following the manufacturer's instructions. RNA libraries were prepared for sequencing using standard Illumina protocols.