bacterial 16 rRNA gene survey: dog feces
DNA extracted from the faecal samples of dogs were used for the amplification and subsequent sequencing of the V1V3 regions of the 16S rRNA gene, as described by Lehtimki et al., 2017 (doi:10.1038/srep45651). The dog population inculded allergic and healthy mothers as well as their puppies of two breeds, Labrador Retriever and Finnish Lapphund, from the rural and urban areas with different lifestyle and diets.