GSM1982469: ESCs D0 rep1; Mus musculus; miRNA-Seq
Small RNAs (smRNAs) were extracted from ES cells and EBs D8 using mirVana™ miRNA Isolation Kit (Ambion, Life Technologies). The concentrations of smRNAs were determined using a NanoDrop ND-1000 Spectrophotometer and the size and purity were assesed using the Bioanalyzer 2100 (Agilent Technologies). Approximately 100ng of miRNA was used for library constructions following the protocol for the Ion Torrent Total RNA-Seq Kit. Briefly, the smRNAs were hybridized and ligated with Ion Adaptor mix, a reverse transcription was performed and the cDNAs were purified using the Nucleic Acids Binding Beads. The amplification of cDNAs was performed by PCR using the Ion 5’ PCR primer and the Ion 3’ PCR primer and the amplified DNA was purified using the Nucleic Acids Binding Beads. Final libraries were used for sequencing analysis on the Ion Torrent PGM instrument using 316 chips.
GEO Accession
GSM1982469