GSM3109355: mESC_ATAC_seq; Mus musculus; ATAC-seq
Cells were trypsinized and disassociated into single cell suspension. 100,000 were collected and incubated in 1ml cold NPB (0.2%NP-40, 5% BSA, 1mM DTT, 1x proteinase inhibitor in PBS) for 15min. NPB treated cells were resuspended in Tagmentation buffer and incubated with 0.5 μl Tagment DNA Enzyme 1 (FC-121-1030, Illumina) for 30min at 37 °C. Tagmented DNA were purified with QIAGEN MiniElute columns. Tagmented DNA were PCR (NEB Next 2x PCR master mix, catalog M0541S)  amplified for 8 cycles using Illumina Nextera adapters. PCR products were purified and size selected for 200~750bp with Agencourt AMPure XP beads.
GEO Accession
GSM3109355