Illumina HiSeq 3000 paired end sequencing; Illumina HiSeq 3000 paired end sequencing; Pig alveolar macrophages gene expression after stimulation             with LPS and Poly (I:C) at 2 and 6 h
RNA-Seq transcriptomic paired end experiment on an Illumina HiSeq 3000
TruSeq Stranded Total RNA kit for Illumina
assay type
transcription profiling by high throughput sequencing
sample storage
frozen, -70 freezer
sample storage processing
cryopreservation, other
sampling to preparation interval
1
experimental protocol
https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseq-stranded-total-rna-workflow/truseq-stranded-total-rna-workflow-reference-1000000040499-00.pdf
extraction protocol
http://www.bea.ki.se/documents/EN-RNeasy%20handbook.pdf
library preparation location
DNA facility, Iowa State University
library preparation location longitude
not provided
library preparation location latitude
not provided
library preparation date
not provided
sequencing location
DNA facility, Iowa State University
sequencing location longitude
not provided
sequencing location latitude
not provided
sequencing date
not provided
experiment target
total RNA
rna preparation 3' adapter ligation protocol
https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseq-stranded-total-rna-workflow/truseq-stranded-total-rna-workflow-reference-1000000040499-00.pdf
rna preparation 5' adapter ligation protocol
https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseq-stranded-total-rna-workflow/truseq-stranded-total-rna-workflow-reference-1000000040499-00.pdf
library generation pcr product isolation protocol
https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseq-stranded-total-rna-workflow/truseq-stranded-total-rna-workflow-reference-1000000040499-00.pdf
preparation reverse transcription protocol
https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseq-stranded-total-rna-workflow/truseq-stranded-total-rna-workflow-reference-1000000040499-00.pdf
library generation protocol
https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseq-stranded-total-rna-workflow/truseq-stranded-total-rna-workflow-reference-1000000040499-00.pdf
read strand
mate 2 sense
rna integrity number
7.9