GSM1446598: Bud after burst replicate 1; Vitis vinifera; miRNA-Seq
Small RNA from green tissues such as leaf, stem, flower, tendril, anther, carpel, was extracted with mirPremierTM microRNA Isolation kit from Sigma, following manufacture’s protocol. Small RNA from bud, berries and rachis, was extracted using the Plant RNA Isolation Reagent (PRIR) method from Invitrogen, followed by the mirPremierTM microRNA isolation kit. A total of 100 mg of ground tissue was used as input material for small RNA extraction in both methods. Small RNA libraries of Corvina's samples were constructed as previously described (Mica et al., 2010) following the Truseq small RNA sample preparation guide (Illumina, USA). Briefly, 50 ng of small RNA was used as input material and ligated first with the 3' RNA adaptor and then with 5' RNA adapter, followed by reverse transcription and 15 cycles of PCR amplification with unique barcoded indexes. Small RNA libraries from the clone PN40024 were constructed following the Small RNA Sample Prep kit (Illumina, USA), starting from 2 µg of small RNAs. The amplified libraries were run on a 6% PAGE gel and purified.
GEO Accession
GSM1446598