GSM3038533: 24hrs MPLA 2; Mus musculus; RNA-Seq
Total RNA was isolated under an RNase-free environment, using the RNeasy Mini Kit (Qiagen). RNA concentration and quality were verified with a Thermo Scientific NanoDrop 2000 spectrophotometer. Total RNA quality was then re-assessed using the 2100 Bioanalyzer (Agilent Technologies). At least 200ng of DNase-treated total RNA with a RNA integrity number greater than 6 was used to generate polyA (mRNA) enriched libraries using TruSeq Stranded mRNA sample kits with indexed adaptors (Illumina). Library quality was assessed using the 2100 Bioanalyzer (Agilent Technologies) and libraries were quantitated using KAPA Library Quantification Kits (KAPA Biosystems).
GEO Accession
GSM3038533