GSM2176475: WT-2; Arabidopsis thaliana; RNA-Seq
Total RNA was isolated using the E.Z.N.A. Plant RNA Kit from (Omega Biotek, Cat#R6827).  To ensure low levels of contaminating ribosomal RNA, two rounds of poly-A mRNA purification were performed using the µMACS mRNA Isolation Kit (Miltenyi Biotech, Cat#130-090-276) Indexed RNASeq libraries were prepared from 100 ng of poly-A RNA starting material using the NEXTflex Illumina qRNA-Seq Library Prep Kit (Bioo Scientific, Cat#5130).
GEO Accession
GSM2176475