GSM2359947: hs63I3_1,KO13; Mus musculus; RNA-Seq
Total RNA from separate biological replicates of three control and two knockout pituitaries was isolated using Trizol (Life Technologies), chloroform extraction, and isopropanol precipitation. Ribosomal RNA was removed using a Ribo-Zero Magnetic Kit (Epicentre). Strand-specific libraries were constructed with ScriptSeq v2 RNA-Seq Library Preparation Kit (Epicentre). RNA-seq deep sequencing was carried out on an Illumina HiSeq 2000.
GEO Accession
GSM2359947