GSM4454834: m2: LFD_rep2; Mus musculus; RNA-Seq
Total RNA was isolated from pancreata tissue samples using the Trizol reagent (Invitrogen), following the manufacturer's instructions. RNA was quantified using the NanoDrop (Thermo ScientificTM NanoDrop 2000) and purity of samples was checked on 1% agarose gels for evaluating the 28S and 18S ribosomal RNA bands (28S/18S ratio). All samples with a ratio (28S/18S) of above 1.8 and an OD 260/280 ratio greater than 1.9 were sent to BGI Company in China for sequencing. RNA integrity number (RIN) was also measured on an Agilent Bio Analyzer 2100 system. Only RNA samples with a RIN > 7 were used for cDNA library construction. All cDNA libraries were sequenced using paired-end strategy (read length 150 bp) on an Illumina HiSeq 2000 platform.
GEO Accession
GSM4454834