GSM1978250: ATAC-seq Acinar3; Homo sapiens; OTHER
Nuclei were isolated from cells by lysing in 50 ul/50,000 cells of lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% NP-40), followed by centrifugation at 500 x g for 10 minutes at 4 degrees Celsius.  Transposition was performed using 50 ul/50,000 cells of transposition reaction mix (25 ul TD buffer, 2.5 ul Tn5 transposase, 22.5 ul nuclease-free water) from the Illumina-Compatible Nextera DNA Sample Prep Kit (Part# FC-121-1030) for 30 minutes at 37 degrees Celsius.  DNA was then purified using the Qiagen MinElute PCR Purification Kit (Part# 28004) and eluted in 10 ul of elution buffer. Libraries were prepared using Nextera barcoded primers and NEBNext High-Fidelity 2X PCR Master Mix (Part# M0541).
GEO Accession
GSM1978250