Fecal microbiota of Holstein cattle in various sampling methods
Genomic DNA was extracted from 200 mg of each fecal sample using an Accuprep Stool DNA Extraction Kit (Bioneer, Daejeon, Republic of Korea) according to the manufacturers protocol and was stored at -20 C until further analysis. The V4 region of the bacterial 16S rRNA gene was amplified from the total extracted genomic DNA. DNA library was constructed using Illumina TruSeq DNA Sample prep Kit.