Metagenomics_of_soil_sample_2_from_Fulani_community
Total genomic DNA was isolated from previously frozen samples using a combination of mechanical and enzymatic lysis followed by bacterial DNA extraction with the MagnaPure LC DNA Isolation Kit III (Bacteria, Fungi; Roche, Mannheim, Germany) according to previously described methods (Klymiuk et al., 2016; Kump et al., 2018) on a MagNA Pure LC 2.0 instrument (Roche Diagnostics, Mannheim, Germany). Hypervariable region V4-specific PCR amplification was performed as described by Klymiuk et al. (2016) using the primer set 515F-5'-GTGCCAGCMGCCGCGGTAA and 806R-5'-GGACTACHVGGGTWTCTAAT synthesized at Eurofins (MWG, Ebersberg, Germany). PCR reaction for each sample was performed in triplicates. Amplicons were purified as previously described (Klymiuk et al., 2016), quantified, pooled, and finally sequenced on a MiSeqII desktop sequencer (Illumina, Eindhoven, Netherlands) using V3 chemistry (MiSeq Reagent Kits v6, Illumina, Eindhoven, Netherlands) and a paired-end protocol according to manufacturers instructions.