GSM3038958: WT_2; Mus musculus; RNA-Seq
Testes were dissected by removing the tunica and snap frozen in dry ice-ethanol. RNA was extracted using RNeasy Plus Micro kit and was further treated with TURBO DNase. RNA libraries were prepared from 500ng total RNA using the Illumina TruSeq polyA v2 kit following the manufacturer's protocol, with the exception that 13 cycles of PCR were performed. The libraries were normalized to 4nM, pooled in an equimolar fashion, distributed uniformly across 2 lanes on high output HiSeq 2500 flow cells using v4 reagents.
GEO Accession
GSM3038958