GSM2439649: MEF_G2; Mus musculus; Bisulfite-Seq
Around 3x10^6 cells were sorted by FACS. DNA was extracted according to the instructions of Wizard® Genomic DNA Purification Kit (Promega A1125). Purity of DNA was determined by K5500 spectrophotometer. DNA quantification was performed using Qubit® 3.0 Fluorometer. Bisulfite-Seq DNA libraries were prepared for sequencing using standard Illumina protocols. Briefly, DNA was fragmented to ~300bp by sonication and followed by end-repair. The blunt, phosphorylated ends were tailed by dA of the 3' end, and the single A overhangs were ligated by the methylated adaptors. Library fragments of ~350 bp were band isolated from 2% agarose gel, converted by bisulfite treatment, and amplified by PCR. The WGBS library was sequenced on an Illumina HiSeq2500 sequencers as paired-end 125-bp reads.
GEO Accession
GSM2439649