GSM3262142: 4A: MAVER-IB-6H; Homo sapiens; RNA-Seq
Total RNA was isolated using RNA mini kit (QIAGEN, Maryland, US) following manufacturer's instructions. All samples were quantified with QubitTM RNA assay (Life technology, Carlsbad, CA) and quality was assessed using Agilent Bioanalyzer (Santa Clara, CA). Library preparations were performed using TruSeq Stranded RNA Sample Preparation kit (Illumina, San Diego, CA). The quality of the library was assessed using D1K ScreenTapes on Agilent TapeStation (Santa Clara, CA) and quantity was assessed by Taqman® qPCR assay directed to the adaptors. Each library was single-end sequenced with read length of 50 bp on Illumina Hi-Seq 2500 at a depth of 30 million reads.  QC metrics were calculated using FastQC (http://www.bioinformatics.babraham.ac.uk/projects/fastqc). Reads were aligned to the transcriptome using STAR and transcript abundance was estimated using Cufflinks. Differentially expressed genes between DMSO and drug-treated conditions were identified using DESeq2.
GEO Accession
GSM3262142