small RNA pool sequencing  from the RISC-bound fraction of Ad4 infected flag-Ago2HEK293 infected cells
small RNA pool sequencing  from the RISC-bound fraction of Ad4 infected flag-Ago2HEK293 infected cells. The cDNA library construction and RNA-seq data was generated by the service provided by the Scilifelab Uppsala (www.scilifelab.se). Enrichment of small RNAs in the size range of 10-40 nucleotides was done by using the PureLink miRNA Isolation Kit (Invitrogen) according to the manufacturer's protocol. RNA samples were treated with tobacco acid pyrophosphatase (TAP), which removes the γ and ß phosphates from the 5´-ends of RNA and thereby generates 5´-monophosphorylated RNAs. Small RNA libraries were constructed using the SOLiD Total RNA-Seq Kit (Rev B, Life Technologies). Strand specific adapters were hybridized to the RNA before reverse transcription. The cDNA was then size-selected on a 10% TBE-Urea gel (Life Technologies) and the libraries were constructed after amplification (15 cycles). Emulsion PCR was performed using the EZ Bead System (Life Technologies) and the small RNA libraries were then sequenced on the SOLiD 5500xl system (35 bp read length, Life Technologies).