T. cacao Matina 1-6 leaf
Total RNA was quantified by fluorimetry.  800 ng was used to prepare a poly dT-primed library optimized for Roche/454 Titanium sequencing that included partial normalization with double-stranded nuclease and PCR suppression of misligated products (Carter et al., in press; prepublication details are available by request to K. Mockaitis, Indiana University).  Reads were cleaned of adaptor sequences using http://sourceforge.net/projects/estclean.