Illumina HiSeq 2000 paired end sequencing; Genome-wide distribution of histones modifications in mouse ESC Rif1+/+ and Rif1-/-
Genome-wide distribution of histones modifications in mouse ESC Rif1+/+ and Rif1-/-
Inducible RsCre Rif1 ES cells have been cultured for two in presence of tamoxifen in order to delete Rif1. ChIP-seq libraries were prepared with NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina (NEB#E6240S/L), except that bead purification was employed (Agencourt AMPure XP beads, Beckman Coulter A63881). Size selection was performed on 2% agarose gels (E-Gel Invitrogen G6610-02). Generally 13 PCR cycles for 10ng starting material were applied. Libraries were barcoded with the NEBNext Multiplex Oligos for Illumina (Index Primers Set 1, NEB#E7335S/L).
Experimental Factor: Rif1 knockout
genotype
Experimental Factor: anti-H3K27-3Me
immunoprecipitate