HG002
A Hi-C library was generated on NA24385 by Arima Genomics using an Arima-HiC protocol that deploys 4 restriction enzymes during chromatin digestion to enable more uniform per base coverage of the genome while maintaining the highest long-range contiguity signal, thereby benefiting analyses such as variant discovery, base polishing, scaffolding, and phasing. After chromatin digestion, digested ends were labelled, proximally ligated, and then proximally-ligated DNA was purified. After the modified Arima-HiC protocol, Illumina-compatible sequencing libraries were prepared by first shearing purified Arima-HiC ligation products and then size-selecting DNA fragments using SPRI beads. The size-selected fragments containing ligation junctions were enriched using Enrichment Beads provided in the Arima-HiC kit, and converted into Illumina-compatible sequencing libraries using the Swift Accel-NGS 2S Plus kit (P/N: 21024) reagents. After adapter ligation, DNA was PCR amplified and purified using SPRI beads. The purified DNA underwent standard QC (qPCR and Bioanalyzer) and sequenced on the HiSeq X following manufacturer's protocols.