GSM4518376: 4T1-HA repl2; Mus musculus; RNA-Seq
Trizol protocol 1000 ng per sample was processed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, E7760L) and included polyA-enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, E7490L), fragmentation for 15 minutes at 94°C prior to first strand synthesis, and 8 cycles of amplification after adapter ligation.
GEO Accession
GSM4518376