GSM3039618: ChowCR 98.3; Mus musculus; RNA-Seq
RNA was isolated from total hippocampus with the trizol method, quantified with Qubit RNA Assay Kit (Life Technologies) and quality controlled with RNA6000 Nano Kit and BioAnalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). Approximately 300 ng was used as input for the Illumina TruSeq Stranded mRNA Sample Prep Kit (Illumina, Inc., San Diego, CA, USA), and sequencing libraries were created according to the manufacturer's protocol. Briefly, poly-A containing mRNA molecules were purified using poly-dT magnetic beads. After purification, the mRNA was fragmented, and first-strand cDNA was produced using random primers and reverse transcriptase. Second-strand cDNA synthesis was then performed using DNA polymerase I and RNase H. The 3' end of cDNAs was adenylated then ligated to indexed Illumina adapters and enriched with PCR to create the cDNA library. The library was then sequenced on a HiSeq 2500 (Illumina, Inc.) instrument as per manufacturer's instructions with 100 cycles of single-end sequencing.
GEO Accession
GSM3039618