DNA-Seq of Homo Sapien: Duodenum
For TCRG (TRG), the LymphoTrack kit (Invivoscribe) was used as per the manufacturers instructions, with 200ng of duodenal biopsy DNA as a template. Primers in the LymphoTrack assays are designed with Illumina adapters. Subsequently, each amplicon was purified by AMPure XP beads (Beckman Coulter, Brea, CA), followed by quantification using a 2100 Bioanalyzer (Agilent). Samples were pooled in an equimolar fashion and sequenced on an Ion PGM (Thermo Fisher Scientific).