Whole-exome sequencing of 7 human intrahepatic cholangiocarcinoma
In this study, we first performed whole-exome sequencing on 7 pairs of ICC tumors and matched normal tissues to detect somatic alterations. These well-characterized patients received no prior anti-cancer treatments and were confirmed to have no background liver or biliary diseases. DNA libraries were prepared according to the Illumina library generation protocol version 2.3. Each sample was tagged with a custom-designed unique 4-base-long index within the Illumina adaptor, pooled, captured by TruSeq Exome Enrichment Kit and sequenced using Illumina Hiseq2000 system.