RNA seq of liver
Three LG samples and three SG samples were used sequencing after three large fish and three small fish were pooled into one sequencing sample in each paddy filed, respectively. Three tissues (B: brain, L: liver and M: muscle) were used to sequence in each sample groups. Total RNA was extracted using the total RNA extraction reagent RNAiso Plus (Takara Bio Inc, Japan) according to the manufacturers protocol. After confirming the quality of RNA with agarose gel electrophoresis and Nanodrop 2000 (Thermo Scientific, USA), RNA that integrity number (RIN) greater than 8 and OD260/280 > 1.88 was used for mRNA library construction.