GSM4455471: S5_DD2_LSK2; Mus musculus; RNA-Seq
BM LSK were FACS sorted from parabiotic mice using Lineage, Sca-1 and C-kit and DAPI labelling, with typical purities > 95%%. RNA was extracted using the Pico Pure RNA Isolation Kit cDNA from LSK cells using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech-Takara). 1 ng of amplified cDNA was used to generate barcoded libraries using the Nextera XT DNA library preparation kit (Illumina).
GEO Accession
GSM4455471