Illumina HiSeq 2000 paired end sequencing; Ageing liver RNAseq of C57BL/6 male mice in dietary restricted and an ad libitum diets
Ageing liver RNAseq of C57BL/6 male mice in dietary restricted and an ad libitum diets
Mice were fed constantly (Ad libitum; AL) while a second group of mice were fed 30% less from 3 months of age (Dietary restricted; DR). Liver tissues were collected from mice of all groups at 3 and 15 months of age. A group of mice from AL and DR at 12 months were moved to the opposite diet and sequenced at 15 months. Strand specific paired end libraries for RNA Seq were generated from DNase treated total RNA using Ribozero and ScriptSeq systems (Epicentre/Illumina)
Experimental Factor: dietary restricted for 9 months then ad libitum
diet
Experimental Factor: 15
age