GSM4089672: BS-16-3wk-Tadpole-Control-1; Xenopus laevis; RNA-Seq
Eyes for optic nerve crush studies were rapidly dissected and then homogenized immediately, pooling six eyes for each biological replicate. Because dissections of hindbrain required more time, each hindbrain was placed in 1 mL of RNAlater at room temperature over-night. RNAlater was then aspirated and five pooled hindbrains homogenized for each biological replicate. Total RNA was isolated using the RNA/DNA/Protein Purification kit from Norgen Biotek Corp. with DNase I treatment, following manufactuer's protocols. Total RNA was enriched for mRNA by ribosomal RNA depletion, which was then used to prepare cDNA libraries using Illumina's TruSeq Stranded Total RNA LT Sample Prep Kit (A&B), with Ribo-Zero Gold.
GEO Accession
GSM4089672