GSM2088377: ATACseq_replicate2; Mus musculus; OTHER
For ATAC-seq, the purified sperm pellet was resuspended in the transposase reaction mix and incubated for 30 min at 37°C. Following incubation, sperm were lysed, incubated with Proteinase K at 55°C for 2 hr, and gDNA was isolated by phenol:chloroform:isoamyl alcohol and EtOH precipitation. Library amplification was done with 2x KAPA HiFi mix and 1.25 µM indexed primers using the following PCR conditions: 72°C for 5 min; 98°C for 30 sec; and 10-11 cycles at 98°C for 10 sec, 63°C for 30 sec, and 72°C for 1 min.
GEO Accession
GSM2088377