GSM4454675: LIB12D; Mus musculus; RNA-Seq
Marrow plug were flushed from femurs and tibias. These long bones were crushed and incubated with 337.5U/mL collagenase (Worthington Biochemical Corp., Lakewood, NJ, USA) and 40 U/mL DNase I (Sigma-Aldrich, NSW, Australia) at 37 °C for 60 min in a shaking water bath. The digested marrow cells and the cells from marrow plug were pooled together. Mononucleated cells were treated with red blood cell (RBC) lysis buffer. Cell suspensions were counted using Countess II FL Automated Cell Counter (Thermo Fisher Scientific). BGI Genomics prepared single cells for 10x Genomics single-cell sequencing 10x Genomics Single-cell RNA sequencing 3' v2
GEO Accession
GSM4454675