Ion Torrent PGM sequencing; A comprehensive map of L1HS-Ta retrotransposons in a panel of human cells obtained by ATLAS-seq.
A comprehensive map of L1HS-Ta retrotransposons in a panel of human cells obtained by ATLAS-seq.
Cells were collected at exponential growth. untreated Genomic DNA was extracted and prepared using QiaAmp DNA Blood mini kit (Qiagen) The protocol is described in Philippe et al. eLife 2016 (PMID 27016617). Briefly, DNA was randomly sheared by sanitation, end-repaired, and A-tailed. After ligating a linker, a suppression PCR step allowed to amplify L1HS-Ta 3' junctions and to add Ion Torrent-specific sequencing primers, as well as sample-specific DNA barcodes for multiplexed sequencing.
Experimental Factor: HepG2
cell line