Illumina HiSeq 2000 paired end sequencing; Meloidogyne incognita Preparasitic J2.1 Transcriptome
Preparasitic J2 were collected as previously described (Rosso et al., 1999).
RNA isolation using TRIzol Reagents (Invitrogen, Carlsbad, CA, USA) was done according to protocol available from Invitrogen and resuspended in 10 ul RNase-free water. Purity and concentration of the RNA was determined on a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) and cDNA was only produced from high RNA quality (RIN superior to 7). Reverse transcription was carried out using the Ovation pico WTA System (NuGEN Technologies, Inc, San Carlos, CA, USA).