GSM3770596: CDK13_R860Q_oe_R160; Danio rerio; RNA-Seq
Library prepped with random priming (NEBNext Ultra RNA Library Prep Kit for Illumina, E7530), fragmentation time of 15min, 12 PCR cycles, and sequenced on an Illumina HiSeq 2000 (100bp paired end reads). Melanomas were homogenized in RLT buffer, subjected to QIAshredder columns (Qiagen, 79656), and then RNA was isolated with a column based method with genomic DNA column removal (Qiagen, 74134).  RNA was ribodepleted (Illumina Ribozero Gold, MRZG12324).  Ribodepletion was confirmed with Agilent 4200 Tapestation.
GEO Accession
GSM3770596