Small RNA deep sequencing of fenpropathrin-resistant strain of Tetranychus cinnabarinus
Qualified total RNA was used to construct the sRNA libraries with TruSeq small RNA Sample Pre Kit (Illumina). Briefly, total RNA was ligated to 5’ and 3’ adaptors, then synthesizing cDNA by reverse transcription. After PCR amplification of the cDNAs, the amplified PCR products within 130–160 bp were separated and purified by a 6% polyacrylamide gel. The Agilent Bioanalyzer 2100 system was used to assess the library quality using DNA High Sensitivity Chips. The qualified libraries were sequenced on a HiSeq2000 sequencer (Illumina).