DNAseq of Paramecium bursaria Km2 :Whole cell
The aposymbiotic strain of Km2 in the early stationary phase was starved for 2 days and collected by water pipe equipped with 11m pore size nylon membrane (NY1102500, Millipore). The cells were washed with modified Dryls solution twice and lysed by equal volume of 0.25 M TCMS buffer (10 mM Tris-HCl pH 8.0, 2 mM CaCl2, 8 mM MgCl2, 0.25 M sucrose) with 0.3% (w/v) NP-40. The DNA was extracted by the QIAGEN Genomic-tip 20/G (Cat No.10223, QIAGEN).