16S rRNA sequencing wastewater community
Genomic Bacteria DNA of the V3, V4 hypervariable region of the 16S rRNA gene was amplified using the barcoded primers described by Klindworth et al., 2013. The PCR was performed with Q5 High-Fidelity 2X Master Mix (NEB) following the protocol provided by Illumina (Illumina, San Diego CA). The PCR products were then purified using the CleanNA magnetic bead PCR clean up kit and 16S rRNA amplicon sequencing was performed using the Illumina MiSeq System.