WGS of Paenibacillus thiaminolyticus Mbale
A PCR-free short read sequence library for the clinical isolate was prepared from 1 g of E220 Focused Ultrasonicator (Covaris, USA) fragmented DNA, average insert size 400 bp, using Hyper Prep Kit (KAPA Biosystems, USA). The final library was diluted to 10 pM and sequenced to 20 million reads with the MiSeq v3 600 cycle reagents (Illumina, CA, USA)