RNA-Seq of mus musculus: adult female lymphocytes
We use ribosomal depletion to remove rRNAs from total RNAsamples, then proceed with library construction using Illumina TruSeqchemistry. Libraries are then sequenced using Illumina NovaSeq 6000 at Columbia GenomeCenter. We multiplex samples in each lane, which yields targeted number ofpaired-end 100bp reads for each sample. We use RTA (Illumina) for base calling and bcl2fastq2 (version 2.20) for converting BCL to fastq format, coupled with adaptor trimming.