GSM4518521: CD69negative_G23; Homo sapiens; OTHER
The plasmids were extracted using QIAGEN Plasmid Plus Midi Kit (QIAGEN, 12945) according to the manufacturer's instructions. The gDNA was extracted from cells using the QIAamp DNA Blood Mini kit (Qiagen, 51106). For each of the NGS library, multiple 50 uL PCR reactions were performed. In each PCR reaction, we used template DNA, 0.25 uM forward primer, 0.25 uM reverse primer, 25 uL NEBNext Ultra II Q5 Master Mix (NEB, M0544S), and nuclease-free H2O up to 50 uL.
GEO Accession
GSM4518521