GSM3107885: AB3298; Mus musculus; RNA-Seq
Each mouse was euthanized and bone marrow harvested by crushing of the femur, tibia and ilia. Cell suspensions were stained with FACS antibodies and single cells sorted into capture plates (Jaitin et al, Science 2014) Antibodies used: Lin = Ter119 (TER-119), CD11b (itgam), Gr-1 (RB8/8C5), CD3 (17A2), CD4 (GK1.5), CD8a (53-6.7), B220 (RA3/6B2); and cKit (2B8), Sca1 (D7), CD34 (RAM34), Flt3 (A2F10), FcgR (93), CD150 (TC15-12F12.2), CD24 (M1/69), CD11c (N418), I-A/I-E (M5/114), CD19 (1D3), NK1.1 (PK136), CD71 (R17217), SiglecH(551), CD22 (OX-97) 3' end mRNA libraries were prepared for sequencing using CRISP-seq (Jaitin et al, Cell 2016) DNA sequencing of guide RNA targeted loci for several CRISPR pools. Libraries were generated using primer flanking the target region for bulk population of cells, and sequenced.
GEO Accession
GSM3107885