GSM3402522: Hypoxia_rep2_RNA-seq; Homo sapiens; RNA-Seq
Cells were cllected on ice and RNA was extracted from each sample using TRIzol reagent (Invitrogen, 15596-026, USA) according to the manufacturer's instruction. The concentration and quality of each sample was measured by Agilent Technologies 2200 TapeStation (Life Technologies, USA). NEBNext ultra directional RNA Library Prep kit, NEBNext Poly (A) mRNA magnetic isolation module and NEBNext multiplex oligos (NEB, USA) was used with 1 ug of total RNA for the construction of sequencing libraries.
GEO Accession
GSM3402522