GSM4295051: siPAPα/γ_Rep_1; Homo sapiens; RNA-Seq
RNA was harvested using TRI reagent (Molecular Research Center, Inc.) according to the manufacturer's protocol. Total RNA was purified using Chloroform-isopropanol extraction. RNA samples were quantified using Nanodrop (Thermofisher) and RNA integrity was checked with Agilent TapeStation (Agilent Technologies, Palo Alto, CA, USA). RNA sequencing libraries were prepared using the KAPA Biosystems Stranded mRNA-Seq kit as per manufacturer's protocol. Briefly, mRNAs were first enriched with Oligod(T) beads. Enriched mRNAs were fragmented for 6 minutes at 94 °C in the presence of magnesium. First strand and second strand cDNA were subsequently synthesized. cDNA fragments were end repaired and adenylated at 3'ends, and universal adapter was ligated to cDNA fragments, followed by index addition (NEBNext multiplex Oligos for Illumina) and library enrichment with limited cycle PCR. The sequencing library was validated on the Agilent TapeStation (Agilent Technologies, Palo Alto, CA, USA), and quantified by using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA).
GEO Accession
GSM4295051