GSM1162041: Setdb1_WT_5hmC_capture-seq; Mus musculus; OTHER
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Genomic DNA was fragmented by sonication of harvested mESCs and size selected (~300bp). The purified DNA was treated with β-glucosyltransferase (β-GT), which transfers an azide-glucose to 5-hmC. The purified products are then biotinylated by click chemistry. This allows the labelled 5hmC containing DNA fragments to be captured by using streptavidin beads. The DNA is purified and sequenced following standard protocols from Illumina.
GEO Accession
GSM1162041