RNA-Seq of Alfalfa under heat stress
Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) following manufacturers recommendations and index codes were added to attribute sequences to each sample. First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase?RNase H-. Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H.