GSM3400801: HeLa_CRISPR-PTEN_RNAPII; Homo sapiens; ChIP-Seq
ChIP-seq: Lysates were clarified from sonicated nuclei and prtein-DNA complexes were isolated with antibody RNA-seq: RNA was extracted from cell pellets using Qiagen RNeasy Kit. ChIP-seq: 2-10 ng of DNA were subject to end-repair, A-tailing, and ligation with Illumina Truseq adapters using NEB enzymes (NEB), followed by size-selection of 300-400 bp and amplification for 10-15 cycles using the KAPA HiFi Library Amplification Kit (Kapa Biosystems). RNA-seq: Illumina TruSeq RNALibrary Prep Kit v2 (RS 122-2001) was used with 1 ug of total RNA for the construction of sequencing libraries
GEO Accession
GSM3400801