RNA-Seq of Caerostris darwini: adult female major ampullate gland
Total RNA was extracted from the major ampullate glands of a single adult female by homogenization in TriZol and cleanup using Qiagens RNeasy kit. DNA was removed from RNA with a TURBO DNase kit (Ambion). The total RNA was submitted to the University of Massachusetts Medical School Deep Sequencing Core Laboratory, where cDNA was synthesized using the Clontech SMARTer cDNA synthesis kit (Takara Bio USA). The cDNA was fragmented, end repaired, and prepared for sequencing by ligating on standard full length Illumina P5 and P7 adapters onto the cDNA inserts and following standard TruSeq Illumina library preparation procedures.