GSM4202007: 3-200 (total RNA); Homo sapiens; RNA-Seq
Cells were lysed in TRIzol, then RNA was extrated using Direct-Zol RNA MiniPrep kit (Zymo Research) NEBNext® rRNA Depletion Kit (New England BioLabs, Ipswich MA USA, E6310) sequencing libraries were prepared from 500ng RNA using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina. 150 paired-end base-pair reads were sequenced using the Illumina NEXTseq 500 (Illumina, San Diego CA USA).   NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina
GEO Accession
GSM4202007