Schistosoma mansoni Male Schistosomulum stage 2 RNA-Seq replicate 2
cDNA library constructions and sequencing were performed at the sequencing facilities of Montpellier GenomiX (MGX, France). PolyA cDNA single strand (positive strand, 50 nucleotides) libraries were constructed from 300ng of total RNA for each sample with TruSeq® Stranded mRNA Sample Preparation kit (Illumina Inc., USA). The quantitative and qualitative analysis of the library was carried on chip Agilent_DNA 1000 chip and qPCR (Applied Biosystems 7500, SYBR Green). The sequencing was performed on a HiSeq2000. Samples were multiplexed by three on the seven lanes of a same flow cell.