Illumina MiSeq sequencing; qiita_ptid_4452:11653.YDST0977
Fecal microbiota of rhesus macaques that received three different vaccination schedules was analyzed. Fecal samples were collected at two time points: soon after birth and when they were juvenile.
Sequences were amplified using F515/R806 primer pair to target V4 region of 16S rRNA gene Both forward and reverse primers were modified to contain an illumina adapter region for sequencing on the MiSeq platform.
barcode
AAGGTAGC
center_name
UC Davis Slupsky lab
center_project_name
monkey.vaccination.project
linker
GT
linker_primer_sequence
GTGTGCCAGCMGCCGCGGTAA
primer
GTGCCAGCMGCCGCGGTAA
run_center
UC Davis Genome Center
run_date
10/6/16
target_gene
16S rRNA
target_subfragment
V4