ChIP-Seq of A. thaliana: stage 6/7 inflorescence from AP1:AP1-GR ap1 cal
Arabidopsis thaliana AP1:AP1-GR ap1 cal and AP1:AP1-GR ap1 cal ANT:ANT-VENUS ant-4 plants treated by pipetting a dex (10mM dexamethasone +0.015% Silwet) solution onto inflorescences. Samples are inflorescences from stage 6/7 plants. Samples for Input DNA were removed prior to addition of GFP (Invitrogen A6455) coated Dynabeads to immunoprecipitate ChIP DNA. Sequencing libraries were prepared from two biological replicates of Input and ChIP DNA for both AP1:AP1-GR ap1 cal and AP1:AP1-GR ap1 cal ANT:ANT-VENUS ant-4 using Accel-NGS 2S DNA library kit (Swift Biosciences). Libraries were sequenced on an Illumina HiSeq 2500 producing 150 base paired-end reads.