RNA-seq of Syngnathus scovelli EE2 exposed adult male muscle/skin
Total RNA was sent to the Michigan State University RTSF Genomics Core where libraries were prepared using the TruSeq mRNA Library Prep Kit v2. Libraries were tested for quality control using Caliper GX and qPCR methods. All 30 individuals were barcoded, allowing for each individuals sequence data to be recovered. The 30 libraries were sequenced using two lanes of an Illumina HiSeq 2500 Rapid Run flow cell v1. Base calling was done by Illumina Real Time Analysis (RTA) v1.17.21.3 and output of RTA was demultiplexed and converted to FastQ with Illumina Bcl2fastq v1.8.4