RNA-Seq of  Solidago canadensis: leaf
The purified isolated RNA fragments  were transcribed into first-strand cDNA using reverse transcriptase and random hexamer-primers and subsequently second-strand cDNA synthesis was performed using DNA polymerase I and RNase H. The purificated and end-repaired fragments were ligated to sequencing adaptors and amplified by PCR.  The library preparations were sequenced on an Illumina HiSeq X-Ten platform.