GSM3108826: shCSL4 HDF rep2; Homo sapiens; RNA-Seq
Total RNA was extracted 7 days after shRNA infection using the directZol RNA miniprep kit (Zymo Research) with on-column DNase treatment. RNA quality was verified on the Bioanalyzer (Agilent Technologies) with RNA integrity number (RIN) > 8.  For shUPF1 experiments, 500 ng of total RNA were depleted of Ribosomal RNA using Ribo-zero RNA removal kit (Illumina) and were used for library preparation using a Truseq kit (Illumina).  For shCSL experiments, 1 μg of total RNA was used for library preparation using a NEBNext Ultra DNA Library Preparation kit (New England Biolabs). A single read was done on the IlluminaHiSeq 2000 sequencer at the Genomic Technologies Facility (Lausanne University).
GEO Accession
GSM3108826