GSM2948178: SNSseq_10d_exp1_F3; Arabidopsis thaliana; OTHER
Nuclei were isolated and enriched by percoll gradients. After lysis, genomic DNA was extracted, denatured and fractionated in a sucrose gradient. Short nascent strands were purified by Lambda exonuclease digestion. ssDNA was converted to dsDNA using a slow annealing strategy. DNA libraries were prepared using Ovation Ultralow V1 library prep kits (NuGen). RNA libraries were prepared using TruSeq Stranded mRNA library prep kit and NeoPrep (Illumina)
GEO Accession
GSM2948178