V3/V4 ribosomal16S microbiome sequencing: soil
Targeting of the bacterial V3/V4 16S ribosomal region for PCR and sequencing. Procedures were according to the Illumina 16S Metagenomic Sequencing Library Preparation (Part # 15044223 Rev. B) (https://support.illumina.com/documents/documentation/chemistry_documentation/16s/16s-metagenomic-library-prep-guide-15044223-b.pdf), targeting V3/V4 regions with the sequences S-D-Bact-0341- b-S-17 and S-D-Bact-0785-a-A-21 described by Klindworth et al. 2013.