RNASeq of prunus salicina Sweet Miriam stage S4 Biological Rep 3
Three biological replicates from Japanese plum cultivar  SM and from  developmental stage S4  were used to extract high quality RNA using the CTAB/NaCl method (Chang et al., 1993) with some modifications Kim et al. (2015b). Library analysis, quantification and the paired-end RNA-sequencing was carried out according to (Kim et al., 2015b): Barcoded Illumina RNA-Seq libraries were created using the TruSeq RNA Sample Preparation Kit V2. Libraries were analyzed with a Bioanalyzer 2100 instrument , quantified by Qubit fluorometer, and pooled in equimolar ratios according to the fluorometric measurements. The pooled library was quantified by qPCR with a Kapa Library Quant kit and sequenced with paired-end 100 bp reads on two lanes of an Illumina HighSeq 2500 instrument.