single-end sequencing raw reads from regenerating Dlac trunk wounds at time point 48h post amputation
Total RNA from 8 Dlac trunk fragments at time point 48h post amputation was isolated using the TRIzol (Invitrogen) standard protocol, quality controlled on a Bioanalyzer (Agilent), poly-A selected using Sera-Mag Oligo(dT) beads (Thermo Scientific), library prepared using NEBNext mRNA Library Prep Reagent Set for Illumina (NEB), and processed for Illumina Sequencing (HiSeq 2000).