GSM3109215: MSCATV-Exo-2: exosomes derived from ATV pretreated MSCs; Mus musculus; ncRNA-Seq
A total of 200 ml of conditioned media of each sample was mixed with Ribo™ Exosome Isolation Reagent and exosomes isolation was performed according to the manufacturer's instructions (RiboBio, Guangzhou, China).Total RNA was quantified by the NanoDrop ND-2000 (Thermo Scientific)and the RNA integrity was assessed using Agilent Bioanalyzer 2100 (Agilent Technologies).Total RNA from exosomes was used for library preparation and sequencing. Library preparation and sequencing were performed at RiboBio.Briefly, RNA was fragmented to approximately 200 bp. Subsequently,the collected RNAs were subjected to first strand and second strand cDNA synthesis followed by adaptor ligation and enrichment with a low-cycle according to the instructions provided with the NEBNext® Ultra™ RNA Library Prep Kit for Illumina (NEB, USA). The purified library products were evaluated using the Agilent 2200 TapeStation (Agilent Technologies) and Qubit®2.0 (Life Technologies, USA)and then sequencing using a HiSeq30000.
GEO Accession
GSM3109215