Achromobacter spanius stain 6
Using SMARTer Apollo library prep system (Takara Bio, Mountain View, CA) with SMARTer PrepX DNA Library Prep kit, a ChIP-seq script was selected for sequencing library preparation. This script excludes less than ~100 bp inserts in library but retain larger ones for sequencing. In brief, fragmented DNA samples with overhangs were first converted to blunt ends in end repair procedure, and adenylated at 3' ends for TA ligation to sequencing adaptors. The ligated and indexed pre-PCR library was then enriched by 5 cycles of PCR. Using Apollo's PCR cleanup script combined with AMPure XP beads (Beckman Coulte, Brea CA), the amplified library was purified for QC analysis and sequencing.