GSM3109189: Sham_sample_2; Mus musculus; RNA-Seq
GFP+ bead-bound nuclei were resuspended in buffer RLT for RNA extraction using the RNeasy Micro Kit, along with the recommended on-column DNAse digestion (Qiagen 74004).  RNA quality and concentrations were measured using an Agilent Bioanalyzer (Agilent RNA 6000 Pico, 5067-1513) and samples having RIN Scores higher than 7 were used. Total RNA was converted to cDNA and amplified using the Ovation RNA- Seq System V2 (Nugen 7102). All samples underwent SPIA Amplification (single primer isothermal amplification) (Ovation RNA-Seq System V2, NuGen, 7102-08) prior to library preparation (NEBNext Ultra DNA Library Prep Kit for Illumina, NEB, 7370S) Macrogen, Illumina HiSeq2500, Rapid run mode, 2 x 50 bp
GEO Accession
GSM3109189