tsICP4 HSV-1 Productive Infection
MRC5 cells were infected with temperature  sensitive ICP4 HSV-1 (tsKos) at an MOI of 10 PFU/cell for six hours. Samples  were grown at permissive (34), nonpermissive (39.6) or shifted up at 4 hpi  from permissive to nonpermissive. ChIP-Seq for Pol II was performed. Illumina  libraries were constructed for the IP and input material. Illumina sequencing  was carried out at Tufts University Core Facility using HiSeq 2500 to  generate 50 bp single-end reads.