GSM4145705: WT 2; Mus musculus; RNA-Seq
BM cells were extracted by grinding long bones, pelvis, and spine and ST-HSC were sorted on Influx instrument (BD Biosciences, San Jose, CA, USA) into Tri Reagent RT (Molecular Research Center, Cincinnati, OH, USA. RNA was extracted using RNeasy MinElute Cleanup Kit (Qiagen, Venlo, Netherlands). cDNA was synthesized from 20 ng of extracted RNA using SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio Inc., Mountain View, CA, USA). Sequencing libraries were constructed using Nextera XT DNA Library Prep Kit (Illumina, San Diego, CA, USA) following manufacturer's instructions.
GEO Accession
GSM4145705