HSV-1 Productive Infection in  MRC5 cells
MRC5  cells were infected with HSV-1 (KOS) at an MOI of 10 PFU/cell for two, three,  four or six hours and ChIP-Seq for Sp1, TBP, TAF1, and Pol II was performed.  Illumina libraries were constructed for the IP and input material. Illumina  sequencing was carried out at Tufts University Core Facility using HiSeq 2500  to generate 50 bp single-end reads.