Synthetic metagenomic sample, input
Library preparation and sequencing for this BioProject.  The Nextera DNA Sample Preparation Kit (Illumina, San Diego, CA) was used to prepare libraries from input, unbound, and bound/eluted fractions from DpnI pulldown assays. Manufacturer’s instructions were followed for the library preparation except for recommended number of PCR cycles, which were varied according to the amount of DNA. For the synthetic mixture, they were as follows: Input – 7 cycles, DpnI bound – 10 cycles, DpnI unbound – 7 cycles. Libraries were sequenced following the manufacturer’s instructions for the HiSeq 2500 Rapid Run mode to obtain 50 nucleotide readlengths.
mixture
Homo sapiens 96.77%, Oryza sativa 1%, Aspergillus fumigatus 1%, Escherichia coli 1%, Bacillus anthracis 0.1%, Salmonella enterica 0.1%, Streptococcus pneumoniae 0.01%, Shigella flexneri 0.01%, Staphylococcus aureus 0.001%, Yersinia pestis 0.001%, Enterococcus, faecalis 0.0001%, Vibrio cholera 0.0001%