Raw reads in Nanopore
Genomic DNA was extracted and sequenced following the Ligation Sequencing Kit (Nanopore, UK). The DNA was purified, and its quality was assessed by the Qubit 2.0 Fluorometer (Thermo Fisher, USA). The DNA was randomly interrupted and the fragments of 20 kb were enriched and purified. Damaged DNA and ends were enzymatically repaired by NEBNext End Repair/dA-tailed (NEB, UK). Then, a 20-kb library was constructed and sequenced by the Nanopore PromethION platform, according to the manufacturers protocols.