GSM1979834: ht10801b_input_x1_(20151015_1); Homo sapiens; ChIP-Seq
the ht1080-1b fibrosarcoma cell  line was grown in dmem media  using standard protocols.  cells were fixed at a density equal to 2 million/ml using formaldehyde to a final concentration of 1%. cross-linking was quenched with 125mm glycine. chromatin was released from cells by incubating in lysis buffer (1% sds, 10mm edta and 50 mm tris-hcl (ph 8.1)) on ice for 10 min. the resulting chromatin solution was diluted 10 times with ip dilution buffer (1% triton x-100, 2mm edta, 150 mm nacl and 20mm tris-hcl (ph8.1)) and digested with micrococcal nuclease (mnase, sigma, cat # n3755) at 37°c for 45 min. reactions were stopped by addition of egta to a final concentration of 20 mm and edta to 10 mm. the mnase digested chromatin was sonicated as described before (pmid: 24737864). an aliquot containing 100th of the entire chromatine solution was de-cross-linked at 65 degrees c overnight. de-cross-linked chromatin was subjected to rnase a and proeinase k treatment followed by dna extraction.
GEO Accession
GSM1979834