erh1_mmi1_LZ
mRNA was isolated from total RNA using the Dynabeads mRNA Purification Kit,fragmented by 5x first strand buffer, and reverse-transcribed using the SuperScript IIReverse Transcriptase. First-strand DNA/RNA hybrid was precipitated with ammoniaacetate and glycogen to remove free dNTPs. Second-strand cDNA was synthesized inthe second strand buffer using dNTPs with dTTP replaced by dUTP, RNase H, DTT,and DNA Polymerase I, and purified using the Agencourt AMPure XP beads.Sequencing libraries were prepared using NEBNext DNA Library Prep Master MixSet for Illumina following its instruction with an additional step of USER enzymetreatment after adaptor ligation. These libraries were then analyzed with IlluminaHiSeq 2500 in the single read mode.