RADseq of subsocial Stegodyphus lineatus - RawData: Sample Slin2
"Genomic DNA was extracted using the DNeasy Blood & Tissue kit, QIAGEN (Germantown, MD, USA). DNA from each individual was normalized to 20 ng per microliter in 10  microliters and double-digested with Sbfl and MspI (New England Biolabs). Paired-end RAD tag libraries were created following the protocol in Poland et al. (2012) with the following modifications: 0.5  microliters of BSA was added to the Restriction Mastermix and a final AMPure Beads clean-up and size selection step was performed after PCR amplification of the libraries to obtain a size range of approximately 150-600 bp. Each individual sample was barcoded with a unique sequence ranging from 4 nt to 9 nt in length (Poland et al. 2012). To obtain paired end reads (100 bp reads), pools of 40 individuals were sequenced on a single Illumina sequencing lane. Individuals were then cleaned from adapters and demultiplexed.  Note that these individual files have not been processed following the pipeline described in Supplementary Figure 1. "