Illumina HiSeq 1500 sequencing; RNA-seq of chicken spleens after LPS injection
RNA-seq of chicken spleens after LPS injection
Chickens were sacrificed, spleens were collected in ice cold PBS, and kept on ice until immediate RNA extraction. RNA was extracted after homogenization of spleen tissue in TriFast reagent (PeqLab) with Precellys ceramic beads (VWR). Homogenization was carried out using two steps of 20 seconds at 6500rpm with a five minute cooling period in between and afterwards. Extraction of RNA from homogenized sample was performed according to the manufacturer's instruction for use of TriFast Reagent. A cDNA library was prepared from 1 ng total RNA using the Sense mRNA Seq Library Kit V2 (Lexogen) according to the manufacturer's instructions.
Experimental Factor: compound
lipopolysaccharide
Experimental Factor: dose
10