Fallow cucumber in which none cover crops-1
Amplicons were extracted from 2% agarose gels and purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, U.S.) according to the manufacturers instructions and quantified using QuantiFluor -ST (Promega, U.S.). Purified amplicons were pooled in equimolar and paired-end sequenced (2  250) on an Illumina platform according to the standard protocols.