Gene expression of macroconidia from M. oryzae 70-15
For RNA isolation, vegetative hyphae were harvested from 2-day-old 5xYEG cultures as described 23.  Microconidia from 7-day-old PDB cultures and macroconidia from 10-day-old oatmeal plates were prepared as described above.  Total RNA was isolated with the Qiagen RNeasy Plant Mini kit.  Library construction with the TruSeq™ RNA Sample Preparation Kit (Illumina) and sequencing with an Illumina HiSeqTM 2000 sequencer were performed at BGI-Shenzhen.  For each sample, at least 7 Mb high-quality reads were obtained.  The cleared reads were mapped to the corresponding M. oryzae 70-15 genes with the Short Oligonucleotide Analysis Package (SOAP) v2.21 program (http://soap.genomics.org.cn).