ITS-1 region fecal microbiome cattle fescue toxicosis
Library preparation and amplicon sequencing was performed using Illumina MiSeq sequencing platform as with the 16S analysis. The ITS1 region was amplified using ITS1f-ITS2 primers designed to amplify fungal microbial eukaryotic lineages designed by the Earth Microbiome Project. This generated paired-end reads of 251bp.