Illumina HiSeq 2000 sequencing; Chromosome conformation capture in mouse embryonic Rif1 null stem cells
Chromosome conformation capture in mouse embryonic Rif1 null stem cells
Chromosome conformation capture was performed starting from 1x107 cells, cross-linked in 2% formaldehyde. Inducible RsCre Rif1 ES cells have been cultured for two and four days in presence of tamoxifen Primary restriction digest was performed by incubation with HindIII, while for the secondary digest we have chosen the 4-bp cutter DpnII.
Experimental Factor: genotype
Rif1-/-
LIBRARY_STRAND
not applicable