Illumina Genome Analyzer IIx sequencing; Meloidogyne incognita Male Transcriptome
Preparasitic males were collected as previously described (Snyder et al., 2006).
RNA isolation using TRIzol Reagents (Invitrogen, Carlsbad, CA, USA) was done according to protocol available from Invitrogen and resuspended in 10 ul RNase-free water. Purity and concentration of the RNA was determined on a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) and cDNA was only produced from high RNA quality (RIN superior to 7). Reverse transcription was carried out using the Ovation pico WTA System (NuGEN Technologies, Inc, San Carlos, CA, USA).