16S high throughput sequencing of archaea in marine sediments
The hypervariable V4+V5 region of the 16S rRNA gene for archaea was amplified using the universal primers (Cheng et al., 2009). The PCR amplification was performed as described by Zhou et al. (2017). Sequencing was performed on an Illumina Hiseq Miseq platform using a paired-end 250-bp sequencing read run with a Miseq Reagent Kit v3 at the Personal Biotechnology Company (Novogene, Tianjin, China).