GSM3402034: T113_GARD_BATCH_2; Homo sapiens; RNA-Seq
Total RNA was extracted from the non-stimulated and stimulated cells using the  miRNeasy kit (Qiagen). RNA quantity was evaluated spectrophotometrically, and the  quality was assessed with the Agilent 2100 Bioanalyzer (Agilent Technologies). Only samples with no evidence for RNA degradation (RNA integrity number > 8) were kept for further experiments. Indexed cDNA libraries were pooled (6 libraries per pool) in  equimolar amounts and sequenced with single-end 100bp reads on an Illumina  HiSeq2500.
GEO Accession
GSM3402034