GSM4091086: DSS miR-494 KO; Mus musculus; RNA-Seq
Following euthanasia of DSS-treated mice, the entire colon was removed, everted, and rinsed 5 times with cold PBS (supplemented with 2% (vol/vol) penicillin and streptomycin). The rinsed colon was immersed into chilled (0-4 ºC) cell recovery solution buffer (354253, Corning, NY, USA) for 30-40 min; the resulting cell suspension was filtered through 70-μm strainers and rinsed with basic cell culture medium (DMEM/F12 (GibcoTM, Waltham, MA) supplemented with 1% (vol/vol) penicillin and streptomycin (GibcoTM), 1% glutamax (Gibco GibcoTM), and 1% HEPES (GibcoTM)) until the supernatant remained clear after centrifugation at 200xg for 2-3 min. RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM4091086