GSM3037959: RASMC_RNA_PDGF_JQ1_2H_H; Rattus norvegicus; RNA-Seq
RNA was isolated using the High Pure RNA Isolation Kit (Roche 11828665001). RNA samples were spiked with ERCC RNA Spike-In Mix (Ambion, Life Technologies) as per the manufacturer's instructions. The dilution of spike-ins was proportional to the amount of RNA from 500k cells per group. RNA was sent to WIGTC for NGS library preparation (Illumina TruSeq) and sequencing (HiSeq 2500). TruSeq Stranded mRNA Library Prep Kit (Cat. No. RS-122-2101, RS-122-2102, and RS-122-2103) was used according to manufacturer's instructions polyA RNA-seq with 40bp single end reads
GEO Accession
GSM3037959