GSM4455708: RNAseq_GM02164_rep1; Homo sapiens; RNA-Seq
Total RNA from 5 million cells was extracted using the NucleoSpin RNA kit (Macherey-Nagel) and its concentration was measured by Nanodrop (NanoPhotometer NP80, Implen). RNA quality was assessed on a Fragment Analyzer (Agilent Technologies) and all of the RNA samples had a RQN between 8.7 and 10. RNA-seq libraries were prepared using 500 ng of total RNA and the Illumina TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold (Illumina) according to the manufacturer's protocol. Multiplexed samples were pooled in equimolar amounts and 2 x 100 bp paired-end sequencing was performed on Illumina HiSeq 2500.
GEO Accession
GSM4455708