GSM2360153: MCF 10A Hygro v. #1; Homo sapiens; RNA-Seq
RNA was isolated by EasyBlue (Intron Biotechnology, Korea) For library construction, we used the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA). Briefly, the steps of strand-specific protocol are: first strand cDNA synthesis; second strand synthesized using dUTPs instead of dTTPs; end reapair, A-tailing and adaptor ligation; PCR amplification. Then, each library was diluted to 8 pM for 76 cycles of paired-read sequencing (2 X 75bp) on the Illumina NextSeq 500 per the manufacturer’s recommended protocol.
GEO Accession
GSM2360153