GSM4201650: RNA-seq from Basal2/BMP o.e CAF; Homo sapiens; RNA-Seq
We extracted total RNA from 1.5 × 106 tumor cells using TRIzol reagent (Thermo Fisher Scientific), following the manufacturer's instructions, and treated DNaseI. We constructed RNA-seq libraries using the NEXTflex™ Rapid Directional RNA-Seq Kit (Perkin Elmer), following the manufacturer's instructions. In brief, we selected and fragmented poly-A-tailed mRNA ligated double-stranded cDNA fragments with adapters and amplified them for 9 cycles of PCR. We then performed RNA-seq using the Illumina HiSeq 2500 platform.
GEO Accession
GSM4201650