GSM1826493: OVCAR8_BRD4_rep1; Homo sapiens; RNA-Seq
RNA were extracted from cells using TRIzol Plus RNA Purification Kit (Life Technologies) according to manufacturer’s protocol and treated with PureLink on-column DNase kit (Life Technologies). RNA quality and concentration was determined using NanoDrop Spectrophotometer. RNA samples were reverse-transcribed using SuperScript III Reverse Transcriptase (Life Technologies), Random Primer Mix (New England Biolabs) and RNAse OUT (Invitrogen). Sequencing libraries were prepared using the Illumina Library Prep Kit, starting with an input amount of 1 µg total RNA and following the manufacturer’s recommendations. Strand-specific libraries were prepared using the Illumina Library Prep Kit according to the manufacturer's instructions. After PCR amplification, the libraries were size-selected to 300 +/- 25 bp on a 2% agarose gel (E-Gel EX, Invitrogen).
GEO Accession
GSM1826493