GSM2088007: Subject L14, dysplasia; Homo sapiens; RNA-Seq
Bronchial airway brushings were obtained during autofluorescence bronchoscopy. Autofluorescence bronchoscopy and endobronchial biopsy were used to identify and sample PMLs (if present). The pathological grade of the lesions with the worst histology present was recorded. Total RNA was extracted from bronchial brushings using miRNeasy Mini kit (Qiagen) according to manufacturer's protocol. Sequencing libraries were prepared from total RNA samples using Illumina® TruSeq® RNA Sample Preparation Kit v2. The libraries from individual samples were pooled in groups of four for cluster generation on the Illumina® cBot® using Illumina® TruSeq® Paired-End Cluster Kit. Each sample was sequenced on the Illumina® HiSeq® 2500 to generate paired-end 100 nucleotide reads
GEO Accession
GSM2088007