GSM4087816: RNA-YBX1-null-175-1; Mus musculus; RNA-Seq
Total RNA was isolated from cultured cells and mouse neural tubes (after SOX2-positive FACS sorting from E13.5 embryos) using Ribozol (VWR) and purified using the Direct-zol RNA  Purification Kit (Zymo Research). DNA digestion with DNase I was performed as suggested and RNA concentration and purity was measured by NanoDrop (ThermoFisher). total RNA with the TruSeq Stranded Total RNA Library Prep Kit were used according to the manufacturer's directions (Illumina, San Diego, Ca.) Paired end 100 cycle sequencing was performed on HiSeq 2000 or HiSeq 4000 sequencers according to the manufacturer's directions (Illumina.)
GEO Accession
GSM4087816