GSM3402074: Old Blood 1; Mus musculus; RNA-Seq
Blood samples were drawn from four young and four old mice. Samples from two mice within each age group were pooled, resulting in two pooled young and two pooled old samples. Subsequently, the pooled samples were diluted 1:1 with PBS+2% FBS and loaded into SepMate-15™ tubes with 4.5 mL of LymphoprepTM with a density of 1.077g/mL (StemCell Technologies). Cells were centrifuged at 1200x g for 20min at 4℃. The top layer was collected and centrifuged at 300xg for 8 min. Supernatant was removed and cells were resuspended in PBS + 2% FBS and this step was repeated. Subsequently, red blood cell lysis was performed using the Red Blood Cell Lysis Solution (Miltenyi Biotec) following the manufacturer's protocol that includes the washing step. Single-cell RNA-seq was performed using 10x Genomics 3' expression protocol
GEO Accession
GSM3402074