Metabarcoding of mock community amplified with emPCR
Genomic DNA was extracted from sixteen fungal isolates using three different methods (Qiagen Dneasy Plant Mini Kit, peqGOLD Tissue DNA Mini Kit, MasterPure Yeast DNA Purification kit). Two-step emulsion PCR (emPCR) of the mock community was conducted using the Micellula DNA Emulsion kit (Roboklon GmbH): a first amplification of 25 cycles, with 2 l of the cleaned template used in a second 25 cycle PCR.