RNA-Seq of turbot: Enteromyxum-infected thymus (24dpi)
RNA extraction was performed using the RNeasy mini kit (Qiagen) with DNase treatment following the manufacturer's instructions. RNA quality and quantity were evaluated in a Bioanalyzer (Bonsai Technologies) and in a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies Inc.), respectively. Samples were barcoded and prepared for sequencing by i) the Wellcome Trust Centre for Human Genetics (Oxford, UK).