RNA-seq of  Calu1 Control cells, replicate 2
A549 and Calu1 cells were transfected with synthesized 5-IleAAT-8-1-L20 mimics or siRNA using 6-well plates with GeneMuteTM reagent.Total RNA of Control  and treated cells were extracted using TRIzol regent (Ambio) according to the Ambion standard RNA isolation procedure, and resuspend in RNase free water. Libraries were constructed using the NEBNext Ultra Directional RNA Library Prep Kit (NEB, Cat# E7420) for Illumina according to the manufacturers instruction.