Illumina HiSeq 2000 paired end sequencing; Rice perception of symbiotic arbuscular mychorrhizal fungi requires the karrikin receptor complex
Rice perception of symbiotic arbuscular mychorrhizal fungi requires the karrikin receptor complex
The hebiba mutant in the Oryza sativa ssp. japonica cv. Nihonmasari background is described in M. Riemann et al., Plant J. 74, 226 (2013) Seeds from wild type and hebibaAOC plants were sterilized with 0.3% Sodium Hypochlorite and pre-germinated on 0.9% bactoagar plates at 30 degrees C. Seven day old seedlings were transferred to sterilized sand and grown for two weeks without fertilizer. Plantlets were then transferred to liquid 12 Hoagland containing 25M phosphate for another week before treating them either with GSE, karrikin 2. Plants were treated with either with Germinated Spore Exudates, karrikin 2 (at a final concentration of 1microM, diluted from a stock of 10mM in 50% methanol) or non-supplemented 12 Hoagland (mock) RNA was extracted as described in C. Gutjahr et al., Plant Cell 20, 2989 (2008).
Experimental Factor: Mock
compound