Amplicon of I. fasciculata microbiome: Cabrera 2014
Amplification of V3-V4 hyper-variable region of the 16S rRNA gene was done in one-step PCR using primers 341F (Muyzer et al. 1993)- 806R (Caporaso et al. 2011). PCR products were normalised and cleaned with the "SequalPrep Normalization Plate Kit" (Invitrogen). Sequening was preformed at the Institute of Clinical Molecular Biology (IKMB), University Kiel, Germany.