Hepatopancreas Transcriptome of Grass Carp (Ctenopharyngodon idellus) fed with lard oil
All the hepatopancreas were immediately frozen in liquid nitrogen and stored at -80 ℃ until use. The equivalent amounts of hepatopancreas samples from fish fed LO diet were combined into one pool, named A1. Similarly, pool B1 was from fish fed FO diet. Total RNA was extracted from these pools using RNAiso Plus (TaKaRa, Dalian, P.R.China) according to the manufacturer’s instructions. The quality and quantity of total RNA were measured using agarose gel electrophoresis and spectrophotometer (NanoDrop 1000, Thermo Scientific, Wilmington, USA). The pools were treated with RNase-free DNase (TaKaRa, Dalian, P.R.China) to remove DNA contaminant. Only RNA samples with a 260/280 ratio from 1.9 to 2.1 and the concentrations greater than 400 ng/μL (gross ＞ 20 μg) were used for the next analysis.