WGS of human gut metagenome: Active Crohn's disease, post-FMT sample (4 weeks), non-responder
DNA extractions from stool were carried out using the QIAamp DNA Stool Mini Kit (QIAGEN, Inc., Valencia, CA, USA). Metagenomic DNA samples were quantified by Quant-iT PicoGreen dsDNA Assay (Life Technologies) and normalized to a concentration of 50 pg μL-1. Illumina sequencing libraries were prepared from 100-250 pg DNA using the Nextera XT DNA Library Preparation kit (Illumina) according to the manufacturer’s recommended protocol, with reaction volumes scaled accordingly. Insert sizes and concentrations for each pooled library were determined using an Agilent Bioanalyzer DNA 1000 kit (Agilent Technologies)