RNA-seq of Cedrela odorata leaflets for transciptome assembly.
Fresh leaf tissue was collected from Cedrela odorata L. at NYBG (Table 1; NYBG Accession 683/89; Bronx, NY, USA; Oregon State University Herbarium voucher OSC-V-258305; referred to as CEOD-NYBG), and frozen on dry ice for total RNA and DNA extraction RNA quality and size distribution were assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). To construct a poly(A)-selected RNA-seq library, we used the WaferGen Bio-systems Apollo 324 NGS Library Prep System and 500 ng of total RNA (WaferGen Bio-systems, Fremont, CA, USA). The mRNA and DNA libraries from CEOD-NYBG were uniquely indexed, pooled to equal mass representation, and sequenced using one lane of 100 bp paired-end sequencing on an Illumina HiSeq 3000 (Center for Genome Research and Biocomputing, Oregon State University, Corvallis, OR, USA).