Periplaneta americana (Linnaeus) whole body isolate for RNA-Seq
Adult American cockroaches were obtained from Kosin University, Busan, South Korea. For immunization, each cockroach was injected by log phase E. coli (2 × 106 colony forming units: CFU) suspended in 10 μL of autoclaved 10 mM sodium phosphate buffer (pH 7.4). Cockroaches were reared at 25 ± 1°C for 18 h before total RNA isolation.