GSM3109176: G3; Vibrio harveyi; RNA-Seq
Cell pellets were collected by centrifugation, total RNA was extracted with TRIzol reagent (Ambion) and PureLink RNA Mini Kit (Ambion) and treated with DNAseI (Invitrogen). The libraries were prepared from total RNA by using ScriptSeq Complete kit (Illumina) optimized for bacterial RNA. The kit included the Ribo-Zero™ (Bacteria) rRNA removal kit and the ScriptSeq™ v2 Kit with ScriptSeq Index PCR Primers Set. The preparation of libraries was carried out following the manufacturer's instructions.
GEO Accession
GSM3109176