GIAB SOLiD 5500W: HG002_NA24385_son: Sample HG002
Design: Duplicate libraries were constructed from each of the first and last vials of the NIST Reference Material 8391, Human DNA for Whole-Genome Variant Assessment, components HG-002 and HG-005. Libraries W2F4 and W2L4 are from HG-002 and represent the first and last (10,728) vials, respectively, of the initial lot of material.  Libraries W5A1 and W5F1 (10,329) are from HG-005 and represent the first and last vials, respectively, of the initial lot of material.  Libraries were prepared manually for HG-002 with the Life Technologies 5500 SOLiD Fragment Library Core Kit (PN 4464412). For HG-005 libraries were prepared using the automated AB Library Builder System with the Life Technologies Library Builder Fragment Core Kit for 5500 Genetic Analysis Systems (PN 4463763).  Library conversion for measurement on the Life Technologies 5500W Genetic Analyzer was completed using the Life Technologies 5500W Conversion Primers Kit (PN 4478020). Individual libraries were sequenced on individual lanes of a flowchip on a 5500W Genetic Analyzer. Sequencing the 5500W libraries was completed with 75 base single-end reads using the 5500W Forward SR 75 Reagent (PN 4475685). 24 lanes (4 Flowchips) per genome were sequenced.   5500W generated .xsq files were mapped to the hg19 assembly using Life Technologies LifeScope Software (version 2.5).  Mapped data resulted in total coverage of 78.7X for HG-002 and 62.2X for HG-005.  Mean mapped read lengths were 57 bases and 55 bases for HG-002 and HG-005, respectively. All .bam files for the individual genomes were merged.  Vial and run information in maintained in the Read Groups.