GSM2441446: AGO1IP-Mock-br2; Arabidopsis thaliana; ncRNA-Seq
RNA was extracted with TRIzol reagent (Life Technologies, USA, 15596018) Libraries were made using the NEBNext Small RNA Library Prep kit (NEB, USA) according to manufacturer’s instructions. Briefly, 3' and 5' adapters were ligated sequentially to the small RNAs. Ligated small RNAs were converted to cDNA by reverse transcription followed by PCR amplification for 15 cycles. The barcoded libraries were sequenced on the Illumina NextSeq500.
GEO Accession
GSM2441446