GSM3262436: CRIST Cas9 IgG control; Mus musculus; OTHER
Cells were treated with formaldehyde to fix the RNA-chromatin structure. RNAs were reverse transcribed in situ using biotin-dCTP. The cDNA-promoter chromatin complexes were immunoprecipitated with an anti Cas9-FLAG antibody and an anti IgG control antibody. cDNAs were then isolated with streptavidin beads. The purified lncDNAs were eluted for Illumina library sequencing. As the control, a Cas9 random gRNA was used in the CRIST assay in parallel with Oct4 and Sox2 promoter specific Cas9 gRNAs. In addition, an IgG control library was also constructed and sequenced as the assay control. Sox2 and Oct4 promoter-interacting lncRNA libraries were prepared using Illumines Kit (Part# 0801-0303).
GEO Accession
GSM3262436