GSM3403189: Q2: Queen 2 (ChIP-seq); Apis mellifera; ChIP-Seq
Tissue was homogenized first using a 22 gauge hypodermic needle and syringe in PBS and then transferred to a dounce homogeniser for 20 strokes of the B pestle. The chromatin extraction, shearing and immunoprecipitation was performed using the Magnify ChIP Kit (Invitrogen). The chromatin was sheared using the Covaris AFA Technology. ChIP experiments were repeated in triplicate and pooled for sequencing (~1 ng/μl). Biological duplicates were created for queen ovary, active worker ovary and worker ovary. DNA for each ChIP reaction for each sample was combined and then air-dried in the speed vac and resuspended in Elution Buffer. The size and concentration of the ChIP DNA was determined with the Agilent 2100 Bioanalyser and the high sensitivity DNA kit. ChIP DNA was sequenced on an Illumina HiSeq 2000 (service provided by Beijing Genomics Institute). ChIP-seq libraries were prepared for sequencing using standard Illumina protocols by BGI
GEO Accession
GSM3403189