GSM4024100: RNA-Seq BEAS-2B FH3.3 WT rep1; Homo sapiens; RNA-Seq
For RNA-seq samples, total RNA was extracted using TRIzol and subsequently continue to prepare RNA-Seq library. For ChIP-Seq samples, mono-nucleosomes were isolated by MNase digestion and sucrose gradient purification from stable cell lines. ChIP was performed using anti-FLAG M2 Affinity-gel. DNA fragments were purified by phenol/chloroform extraction and then continue for ChIP-Seq library preparation. RNA-Seq and ChIP-Seq library preparation were followed by Illumina TruSeq RNA Library Prep Kit or Illumina TruSeq ChIP Library Preparation Kit.
GEO Accession
GSM4024100