GSM4144608: PF-2.5uM-2; Mus musculus; RNA-Seq
Total RNA was collected from cultured cells using QIAshredder (QIAGEN) and PureLink RNA mini kit (InvitrogenTM) following the manufacture's instructions. Lysis buffer with 2-mercaptoethanol was added to cold PBS washed cells and collected into QIAshredder, then centrifuged at 15,000g for 3 minutes. The flow-through was collected into a new tube and RNA isolation was carried out with PureLink RNA mini kit following the manufacturer's instructions.
GEO Accession
GSM4144608