GSM2176386: 4Cm TALE silenced cells rep3; Homo sapiens; MeDIP-Seq
DNA was extracted with Qiagen Kit (Cat.13343) according to manifacturer's instructions. 500 ng of genomic DNA was sonicated with E220 COVARIS ultrasonicator (Covaris) and sequencing libraries were prepared using NextFlex Methylseq kit 1 (Bioo Scientific). After adapter ligation step, samples were pooled and immunoprecipitated using a monoclonal antibody directed against 5-methylcytosine (MagMeDIP kit, Diagenode). Enriched and control libraries (not immumoprecipitated) were purified using the IPure kit (Diagenoide). Enrichment efficiency was evaluated by qPCR using provided internal controls (spiked-in DNA from A. thaliana). Libraries were amplified following NextFlex Methylseq kit 1 protocol
GEO Accession
GSM2176386