GSM4518498: cxcr3.2wt_rep3; Danio rerio; RNA-Seq
3 independent groups of 150-200 5dpf Tg (mpeg1: mCherryF cxcr3.2-/- and cxcr3.2+/+) larvae were dissociated and red fluorescent macrophages were FACS sorted. RNA was extracted using the miRNeasy mini kit (Qiagen) according to the manufacturers' instructions. cDNA synthesis and amplification was done using the SMARTer® Universal Low Input RNA Kit for Sequencing (Clontech) following the manufacturer's guidelines. Approximately 10 Mreads were obtained, using Illimuna Single-Read runs. Reads were then aligned to GRCz10 Danio rerio genome.
GEO Accession
GSM4518498