Soap_assembky
Genomic DNA was extracted using Wizard Genomic DNA Purification Kit and sent to the Georgia Genomics Facility (University of Georgia, Athens, USA) for sequencing. DNA was sheared using Covaris S2, Illumina adapters were ligated on, the libraries were constructed using methods derived from Fisher et al. (2011), using adapters equivalent to Illumina TruSeq, except that the indexes were 10 nt (Faircloth & Glenn, 2012). These were pooled and run on the Illumina HiSeq (paired-end 100 reads, Illumin, San diego, CA) at Giorgia Genomics Facility (University of Georgia). Reads were assembled using Newbler,v 2.9 and CLC-Genomics Workbench 6.5, using the complete mitogenome of Paralithodes camtschaticus as reference (GeneBank accession number  JX944381). Gene annotation was realized using DOGMA (Wyman et al., 2004) and MITOS (Bernt et al., 2013) software. However these program annotated 16 tRNA, which were checked with tRNAscan-SE (Lowe & Eddy, 1997) and RFam (Griffiths-Jones et al., 2003) programs.