GSM1160359: s_1_1_human; Homo sapiens; MNase-Seq
10 million sperm cells were diluted in PBS and incubated with 0.1% Lysolecithin. After 10mM DTT treatment, freed chromatin was digested with 20U micrococcal nuclease and centrifuged at 10000 rpm to separate nucleosomal (supernatant) from protamine fraction (pellet). Total DNA from nucleosomal fraction was isolated and separated on 2% agarose gel. 146 bp mono-nucleosomal DNA fragment was purified from the gel (NucleoSpin-Gel-clean-up-Kit, Macherey-Nagel). 100 ng of mono-nucleosomal DNA were applied for preparation of the DNA library. DNA samples underwent end repair, dA-tailing and adaptor ligation according to the provider's protocol (Library preparation master mix kit, New England Biolabs).
GEO Accession
GSM1160359