Illumina Sequencing of Klebsiella pneumoniae DHQP1701672
DNA bacterial extractions were prepared using the Promega Wizard Genomic DNA Purification Kit (Madison, WI). High quality gDNA was fragmented using the Covaris ME220 Focused-ultrasonicator (Woburn, MA), and sample libraries were prepared using the NuGEN Ovation Ultralow System V2 assay Kit (San Carlos, CA) and the PerkinElmer Zephyr G3 NGS Workstation (Waltham, MA). Whole genome sequencing was performed using an Illumina MiSeq System (San Diego, CA) producing 2 by 250 paired-end reads.