GSM3109107: Gate 2 (green-red) sorted cells of small intestinal crypts isolated from Neurog3Chrono animals, replicate 4; Mus musculus; RNA-Seq
Cells were sorted into Trizol (Life Technologies) and total RNA was isolated according to the manufacturer's instructions, with the following alterations. RNA was precipitated overnight at -20 °C, with 2.5 ug GlycoBlue (Life Technologies). RNA was processed using the previously described CEL-seq technique, with the indicated modifications. Libraries were sequenced on an Illumina NextSeq500 using 75bp paired end sequencing (Samples Neurog3ChronoBulk01-06) or on an Illumina Hiseq4000 using 150bp paired end sequencing (Samples Neurog3ChronoBulk09-16)
GEO Accession
GSM3109107