transcriptome analysis of B. sudeticus prior to and after nitrogen starvation
Cells prior to nitrogen starvation were subjected to total RNA extraction using TRIzol* Plus RNA Purification System (Invitrogen-Life Technologies Co., Carlsbad, CA, USA) according to manufacturer’s protocol.  Appr. 4ug of the resulting total RNA was used for synthesis of cDNA using the TruSeq RNA Sample Prep Kit (Invitrogen-Life Technologies Co.) according to manufacturer’s instruction including synthesis of first and second strands cDNA, end repair, 3’-end adenylation, adapter ligation, fragment enrichment (e.g., ~215bps in length), and library validation, quantification, and quality assessment with a bioanalyzer (Agilent Technologies; Santa Clara, CA, USA).  The libraries are sequenced using the Illumina HiSeq 2000 Sequencer (BGI, Shenzhen, China).