GSM2178494: 10T3M; Homo sapiens; RNA-Seq
Cells lysed in 10 mM Tris-Cl, pH 7.4, 5 mM MgCl2, 100 mM KCl, 1% (v/v) Triton X-100, 0.5% (w/v) deoxycholate, 1000 U/ml RNasin, 2mM DTT and 100 µg/ml Cycloheximide.  Total RNA and RNA from polysomal fractions were isolated with Trizol LS as per manufacturer's instructions. Poly(A) RNA was isolated using Dynabeads mRNA DIRECT Micro Kit (Life Technologies) according to manufacturer’s instructions. Ion Total RNA-Seq Kit v2 (Life Technologies) was used according to manufacturer’s recommendations to prepare libraries.  the Ion PI Template OT2 200 Kit v2 (Life Technologies) was used as per manufacturer’s instructions to generate template Ion PI Ion Sphere particles using the Ion OneTouch 2 System. Ion PI Chip preparation was done using the Ion PI Sequencing 200 Kit v2 (Life Technologies) and sequenced on the Ion Proton Sequencer platform. Single-read RNA-seq
GEO Accession
GSM2178494