GSM2358420: Pollen_bioreplicate_3; Arabidopsis thaliana; ncRNA-Seq
For the Inflorescence libraries, total RNA was extracted using Trizol reagent and enriched using a the MirVana microRNA isolation kit. For the pollen libraries sRNA enrichment was performed through gel purification and elution of the fraction containing the 20-26 nt range as determined by a sRNA size marker (miRNA Ladder,New England Biolabs). For the IP libraries, small RNAs were extracted from proteins using Trizol LS. Cloning of small RNAs was performed using the Illumina TruSeq RNA Sample Preparation Kit.
GEO Accession
GSM2358420