GSM4024116: ES_Dox_H3K27me3_ChIPSeq; Mus musculus; ChIP-Seq
For ChIP, crosslinked chromatin was prepared using standard conditions or the SimpleChIP® Plus Enzymatic Chromatin IP Kit. For WGBS, genomic DNA was isolated by alkali lysis using classical methods. For ChIP libraries, the NEBNext ChIPSeq library kit or the SimpleChIP® Plus Enzymatic Chromatin IP Kit was used as per instructions. For WGBS libraries, DNA was bisulfite treated using the Zymo EZ DNA Methylation Lightning Kit followed by library preparation using the TruSeq DNA Methylation Kit (Illumina).