GSM2947802: maize fertile anthers, 0.5/0.6 mm, infected with U. maydis SG200, rep #4; Zea mays; RNA-Seq
Total RNA for RNA-seq libraries was isolated using the PureLink Plant RNA Reagent (ThermoFisher) following the manufacturer's instructions. Total RNA quality and quantity were accessed by RNA 6000 Pico Kit (Agilent) and by the Qubit RNA BR Assay Kit (ThermoFisher), respectively. An aliquot of 500 ng of total RNA was treated with DNase I (New England BioLabs, Ipswich, MA) and then cleaned with RNA Clean and Concentrator-5 (Zymo Research Corp., Irvine, CA). An aliquot containing 200 ng of treated RNA was used for library construction. The TruSeq Stranded Total RNA with RiboZero-Plant Kit (Illumina) was used for library construction following the manufacturer's instructions. Libraries were sequenced in an Illumina HiSeq 2500 instrument with single-end mode with 101 bp reads. All of the sequencing runs were performed in the University of Delaware Sequencing and Genotyping Center in Newark, DE.
GEO Accession
GSM2947802