whole genome sequencing of Adelie penguin modern sample: Sample CB4.T525
"Genomic libraries of modern Adelie penguin samples were prepared by first extracting DNA from Seutin-preserved blood samples using a modified Qiagen DNEasy Blood and Tissue protocol (Qiagen, Valencia, CA, USA). Extracts were then sheared with a Covaris sonicator (Covaris, Woburn, MA, USA) to an average size of 300-600bp, and adapter-ligated using the standard NEBNext protocol and NEBNext multiplex Illumina adaptors (NEB, Ipswich, MA, USA). Samples were submitted for 101 base pair (bp) paired-end (PE) sequencing on an Illumina HiSeq2000 at BGI-Hong Kong."