Illumina HiSeq 2000 paired end sequencing; Recurrent activating STAT5B N642H mutations in myeloid neoplasms with eosinophilia
Recurrent activating STAT5B N642H mutations in myeloid neoplasms with eosinophilia
Our study included samples from individuals referred for routine diagnostic analysis of persistent unexplained eosinophilia and/or patients diagnosed with MPN or MDS/MPN with eosinophilia according to standard morphological, hematologic and laboratory criteria. Cases that tested positive for FIP1L1-PDGFRA or other recognised TK fusion genes were excluded. The study design adhered to the tenets of the Declaration of Helsinki and was approved by the National Research Ethics Service (UK). Fourteen patients with MPN-eo or idiopathic hypereosinophilic syndrome were recruited. RNA was extracted from total peripheral blood or bone marrow leukocytes using standard procedures. RNA-Seq libraries were prepared from total RNA using poly(A) selection.
Experimental Factor: individual
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