Illumina HiSeq 3000 paired end sequencing; Illumina HiSeq 3000 paired end sequencing; Pig histone modification alias
ChIP-Seq paired end experiment on an Illumina HiSeq 3000
NEBNext Ultra II DNA Library prep kit for Illumina
assay type
ChIP-seq
sample storage
frozen, -70 freezer
sample storage processing
cryopreservation in liquid nitrogen (dead tissue)
sampling to preparation interval
1
experimental protocol
ftp://ftp.faang.ebi.ac.uk/ftp/protocols/assays/ISU_SOP_Fetalliver_20180915.pdf
extraction protocol
ftp://ftp.faang.ebi.ac.uk/ftp/protocols/assays/ISU_SOP_Fetalliver_20180915.pdf
library preparation location
DNA facility, Iowa State University
library preparation date
2018-11-14
sequencing location
DNA facility, Iowa State University
sequencing date
2018-12-04
experiment target
histone_modification
chip protocol
ftp://ftp.faang.ebi.ac.uk/ftp/protocols/assays/ISU_SOP_Fetalliver_20180915.pdf
control experiment
ERX3212555
target protein name
Histone
chip antibody provider
Diagenode
chip antibody catalog
H3K4me3 (Diagenode, catalog: C15410003)
chip antibody lot
A1052D
library generation max fragment size range
600
library generation min fragment size range
200