Illumina HiSeq 3000 sequencing; RNA-seq of age-associated transcriptome changes in brown adipose tissue
RNA-seq of age-associated transcriptome changes in brown adipose tissue
Brown adipose tissue was collected from young C57Bl6 mice (10 weeks old) and old C57Bl6 mice (76 weeks old) treated with or without β3 adrenergic receptor agonist (CL-316, 243) at 1 mg kg-1 under thermoneutrality for 5 days. Young C57Bl6 mice (10 weeks old) and old C57Bl6 mice (76 weeks old) were acclimatized under thermoneutrality at 30 degrees for 7 days and treated with β3 adrenergic receptor agonist (CL-316, 243) or saline control at 1 mg kg-1 for 5 days. Total RNA was extracted from brown adipose tissue of young C57Bl6 mice (10 weeks old) and old C57Bl6 mice (76 weeks old) treated with or without β3 adrenergic receptor agonist (CL-316, 243) using the RNeasy Mini Kit (Qiagen).RNA quality (RIN: RNA Integrity Number) was determined by Bioanalyzer (Agilent Technologies) and samples with RIN of >8.0 were subjected to RNA-seq.  RNA Sequencing libraries were constructed from total RNA using Universal Plus mRNA-Seq (NuGEN).
Experimental Factor: age
76
Experimental Factor: stimulus
none