GSM1444490: in vitro rep3; Burkholderia glumae BGR1; RNA-Seq
The infected rice stem segments were centrifuged directly at 4°C and 2,000 × g for 5 min in 15-ml conical tubes to obtain bacteria pellets. Each pellet contained 2.5 × 108 to 3.3 × 108 CFU/ml, which was primarily composed of B. glumae BGR1 (data not shown). For later RNA extraction, the collected pellets were suspended in 2 ml of RNAprotectTM Bacteria Reagent (Qiagen, Valencia, CA). RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM1444490