GSM2600318: M1_A01; Mus musculus; RNA-Seq
On day 3 post transduction, cells were collected by trypsinization, stained with 7AAD live/dead dye (Thermo Fisher Scientific), and FACS-sorted for live cells (negative for 7AAD). The sorted single cell suspension was loaded on a medium-sized (10-17 µm) microfluidic RNA-seq chip and single cells were captured with the C1 system. Bright field images were taken for each well of captured cells for later assessment of cell number and quality in each well. Next, RNA spike-in was added and cDNAs were prepared on chip using the SMARTer Ultra Low RNA kit from Illumina (Clontech).  Judged by cell number and morphology in the microscopic images, only cDNA libraries from single healthy cells were selected for Illumina library preparation. Illumina libraries were prepared for sequencing using standard Illumina protocols (the Nextera XT Kit).
GEO Accession
GSM2600318