GSM4146175: Sh_D1N_Gn: non-D1R expressing, non-virus infected, AAV5-ZacF-U6-luczsGreen-shShank3; Mus musculus; RNA-Seq
Nucleus Accumbens were removed, cells were dissociated using papain incubation and FACsorted to obtain the 4 types of cells per experiment used in the analysis. To prepare cDNA libraries, collected frozen cells were processed using a Quiagen RNeasy kit (Qiagen, #74034) to extract RNA cDNA libraries were prepared for sequencing using standard Illumina protocols using SMARTseq v4 kit (Clontech, # 634888) and sequenced using HiSeq 2500 in 100 pairbase length fragments for a minimum of 1 million reads per sample.
GEO Accession
GSM4146175