GSM1982477: UC06 Caput HEE cells NC siRNA Rep 1; Homo sapiens; RNA-Seq
Confluent adult Caput HEE cells transiently transfected with siRNA (targeting both HNF1alpha and HNF1beta or a non-targeting control) for 72h, were harvested for RNA extraction using TRIzol. RNA quality was confirmed by Nanodrop measurement of OD 260/280 and 260/230 ratios and stored at -80oC under ethanol. 1ug RNA was used for RNA-seq library preparation. RNA-seq libraries were prepared using the TruSeq RNA Sample Preparation Kit v2 (Low-Throughput protocol, Illumina).
GEO Accession
GSM1982477