GSM2599726: ILB_9579_IonTorrent; Mus musculus; RNA-Seq
Mice were sacrificed and liver samples were dissected and snap-frozen in liquid nitrogen. RNA was extracted from the liver tissue using TRIzol and Qiagen Rneasy Mini Kit, according to manufacturers’ protocols. Ion Torrent: Total RNA was poly-A selected using Ambion’s (ThermoFisher) Dynabeads mRNA Direct Micro Purification kit. About 100 ng poly-A RNA was used to prepare strand-specific barcoded RNA library with the Ion Total RNA-Seq kit v2.0 following manufacturer’s protocol. The library quality was checked by running on the BioAnalyzer and the concentration was determined from the analysis profile. Ten barcoded libraries were pooled together on an equimolar basis and run using three PIv3 chips on an Ion Torrent Proton using HiQ chemistry.    Illumina: 200 ng total RNA from each liver sample was prepared for Illumina sequencing according to the manufacturer’s protocol using the TruSeq Stranded mRNA Sample Preparation Kit.  Following preparation, library quality was assessed using a Bioanalyzer. Libraries from all samples were pooled together and sequenced using an Illumina HiSeq 2500 (125 bp paired-end reads).
GEO Accession
GSM2599726