GSM3768998: SH002_1; Homo sapiens; RNA-Seq
RNeasy Plus Micro kit, (#74034) Qiagen, mRNA was fragmented randomly in fragmentation buffer, followed by cDNA synthesis using random hexamers and reverse transcriptase. After first-strand synthesis, a custom second-strand synthesis buffer (Illumina) was added with dNTPs, RNase H and Escherichia coli polymerase I to generate the second strand by nick-translation library preparation was performed using the NEB Next® Ultra™ RNA Library Prep Kit and enriched using oligo(dT) beads Illumina HiSeq 2500 (mRNA, 2x150bp, paired end, poly-A selected)
GEO Accession
GSM3768998