NextSeq 550 paired end sequencing; T cell receptors repertoire sequencing of the human CD8+ memory subsets (TSCM, TMEX and TEM)
T cell receptors repertoire sequencing of the human CD8+ memory subsets (TSCM, TMEX and TEM)
T cells were FACS-sorted from total PBMCs of six healthy donors in duplicate (150,000 cells/subset for each replicate) in 1.2 mL RLT buffer (Qiagen; final dilution < 20%) for cell lysis and stored at -80°C Total RNA was extracted using RNeasy Mini Kit (Qiagen)  5′ RACE TCRβ sequencing libraries with Unique Molecular Identifiers (UMIs) were prepared using a Human TCR Profiling Kit (MiLaboratory) according to the recommended procedure.
Experimental Factor: cell type
TSCM