RNA-Seq of Dip2c Knockout mus musculus: adult male brain
Sequencing libraries were generated using NEB Next Ultra TM RNA Library Prep Kit from Illumina (NEB, USA) following manufacturer's recommendations. Briefly, mRNA was purified from total RNA; mRNA was enriched via magnetic oligo beads and then fragmented and broken into short fragments. Double stranded cDNA was synthesized by using random hexamer primer. After that, NEBNext sequence adapter containing index sequences were hybridize to the ends of cDNA and the desired fragments were separated using AMPure XP beads ((Beckman Coulter, Brea, CA, USA) . Finally, the four cDNA Libraries were sequenced using BGISEQ-500 platform and 100 bp paired-end reads were generated.