16S-seq of April Barrow seawater incubation: control at T=48h
Experiment assessing the effect of terrestrial organic matter amendments to arctic seawater microbial communities. Seawater or seawater incubation samples were filtered onto 0.2 um filters, frozen, and DNA was subsequently extracted and the 16S rRNA gene was amplified using the 515F-806R primer set. Sample amplicon libraries were subsequently quantified using picogreen and pooled in equimolar concentrations and sequenced on an Illumina MiSeq at Argonne National Lab