GSM4454031: enp1-1 + empty vector 90 minutes replicate 2; Saccharomyces cerevisiae; RNA-Seq
RNA extractions were performed using 5 OD yeast cell pellets. RNA was extracted using the Quick-RNA Fungal/Bacterial prep kit (Zymo Research). Library preparation and sequencing was carried out by UC Davis Genome Center following the 3´-Tag Sequencing method (QuantSeq 3´ mRNA-Seq kits). Sequencing used an Illumina HiSeq 4000 with single end 90 bp reads. The adapters, polyA tails, and the first 12 bases of each read were trimmed according to manufacturer instructions (https://www.lexogen.com/quantseq-data-analysis/).
GEO Accession
GSM4454031