RNA-seq of Arabidopsis thaliana: pollen wild-type repeat 2
The mature pollen grains were collected using a vacuum cleaner, as described previously (Johnson-Brousseau and McCormick 2004). Frozen dry pollen was used for RNA-seq library preparation. The library was prepared using the BrAD-seq method, as described previously (Townsley et al. 2015; Ichihashi et al. 2018). The library was sequenced by the NextSeq500 sequencer (Illumina, San Diego, CA, USA).