Aquila chrysaetos canadensis genome sequencing- unpaired
2cc of blood was collected via venipuncture of the brachial vein and stored in lysis buffer at ambient temperature until shipped to the laboratory at Purdue University.  Three drops of blood were preserved in 1 ml of lysis buffer (100 mM Tris-HCl, 100 mM EDTA, 10 mM NaCl, 2% SDS).  Genomic DNA was subsequently extracted using a standard phenol chloroform protocol and a standard PCR assay was used to confirm sex genetically.  In February and March 2013, we conducted one lane of paired-end sequencing and one lane of mate-paired sequencing using an Illumina HiSeq 2000 that produced read lengths of 100 bp.  The paired-end genomic library was composed of sequences with a mean insert size of 366 bp, whereas the mate-paired genomic library contained inserts with a mean size of 1.4 Kb.