HLGB_63_2007Bact
After centrifugation of the mat/sediment/water slurry sample at 3,900*g for 20 min, approximately 5 g was used for extraction via a protocol modified from Zhou et al. (1996; PMID 8593035) that has been described previously (De Le*n et al., 2012; PMID 22476815).    Bacterial small subunit (16S) rRNA gene amplification was performed using universal bacterial primers 530F (5′-GTGCCAGCMGCNGCGG-3′) and 1100R (5′-GGGTTNCGNTCGTTR-3′) under conditions described in Hwang et al. (2009; PMID 18769457).    All primers had 10-nt barcodes on the 5′ end for sample identification purposes.  Cycle numbers were optimized for each sample to minimize PCR-induced artifacts with 10-12 ng DNA per reaction  Pyrosequencing for the 2007 bacterial amplicons was done with GS-FLX pyrosequencing technology at the Medical Biofilm Research Institute (Lubbock, Texas, USA).  Quality scores are not available for these data.  For the purpose of fastq production, all nucleotides have been assigned an arbitrary quality score of 20.