GSM3261540: Mouse_1cell_embryo_KO_br3; Mus musculus; RNA-Seq
Total RNA was extracted from triplicates of 25 wild type or knock-out GV oocytes, MII eggs or 1-cell zygotes   using a PicoPure RNA Isolation Kit with on-column genomic DNA digestion according to the manufacturer's instructions (Thermo Fisher Scientific, Philadelphia, PA). Each sample was spiked in with 0.2 pg of synthesized Renilla luciferase mRNA before extraction as a normalization control. Non-stranded RNAseq libraries were constructed using the Ovation RNA-seq system V2 (NuGEN, San Carlos, CA) followed by Ovation Ultralow Library system (DR Multiplex System, NuGEN, San Carlos, CA). RNAseq libraries were pooled and sequenced by 125 bp paired-end reading using the Illumina HiSeq at the High Throughput Genomics Core Facility at University of Utah, Salt Lake City, UT.
GEO Accession
GSM3261540