RNA-Seq analysis of ALL sample TARGET-10-PANSFD-09A-01R
Total RNA from 1 tumor sample was extracted using Trizol and DNAse treated. mRNA was purified and fragmented into 150bp size fractions using the Illumina TruSeq Sample Preparation Kit. First and second strand cDNA was synthesized, followed by A-tailing and ligation to RNA Adapter Index 5. The cDNA was PCR amplified, resuspended in 30ul Resuspension Buffer and fraction size assessed by Agilent analysis. Quantification was performed with the Agilent qPCR NGS Library Quantification kit.