94T778FRAP1shRNA7
Briefly, for each clonal population, PCR-free Libraries were prepared using Kapa Hyper Library Prep kit (Cat. # KK8505, Roche) according to the vendor's user guide.All 30 clones were pooled and to avoid batch effects, repeatedly sequenced across five NovaSeqTM 6000 S4 flow cells (Cat.#20012850,Illumina, CA,USA), according to Illumina's user guide. The loading concentration was 200pM.