16S-rRNA Gene Seq: Duodenum
Sequencing libraries were created by the Mayo Clinic Genome Analysis Core (Rochester, MN). Briefly, the V3-V5 region of the 16S rRNA gene was amplified with multiplexing barcodes using PCR (V3-341F: TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGAGGCAGCAG; V5-926R: GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGCCGTCAATTCMTTTRAGT). The libraries were then pooled and size-selected between 700 and 730 bp using a LabChip XT (PerkinElmer, Waltham, MA). Sequencing was performed on a single lane of an MiSeq sequencer (Illumina) using paired-end mode.