GSM4454801: Sample 7: CD200_AIA; Mus musculus; RNA-Seq
Using Smartseq v4 Ultra® Low Input RNA Kit for Sequencing (TakaRa), RNA was reversely transcribed to first strand cDNA which was amplified. ERCC RNA Spike-In Mix (Invitrogen) was added to lysed cell samples, so that final ERCC dilution in RT reaction was 1:65.000 and final dilution in PCR mix was 1:100.000. Amplicons were purified using Agencourt AMPure XP Kit (Beckman Coulter). Quality of cDNA amplicons was assessed using High sensitivity cDNA chip (Agilent) and Bioanalyzer instrument (Agilent). 1 ng of each cDNA amplicon was used for library preparation using Nextera XT DNA Library Preparation Kit (Illumina).
GEO Accession
GSM4454801