GSM3770655: PDLSC-oD+Lev_Rep2; Homo sapiens; RNA-Seq
For isolation of stem cells from the periodontal ligament, which was left over after tooth (third molar) extraction, above all within the center and partially apical third of the root surface, was taken with sterile forceps and scalpels. The tissue was mechanically disrupted and enzymatically digested using 0.4 mg/ml collagenase and 4 mg/ml dispase in sterile PBS (1 h, 37 °C). Supernatant was discarded, and cells were transferred into a 25 cm2 culture flask with standard culture medium including 2.5 µg/ml Amphotericin B (Sigma Aldrich, now Merck KGaA, Darmstadt, Germany). RNA libraries were prepared for sequencing using standard Illumina protocols