rna-seq_sce_n180a
Briefly, we sampled 0.8 mL of cultures at 1.5 OD, pelleted and flash-froze them in liquid nitrogen. RNA extraction was performed with MasterPure» Yeast RNA Purification Kitkit (Epicentre). Library preparation was used with TruSeq Stranded mRNA Library Prep kit from Illumina. Paired-end sequencing of libraries was performed on the Illumina Nextseq 500 platform using a mid-output 150 cycle v2 flow cell.