GSM3037383: 2B3H; Mus musculus; RNA-Seq
Lungs were perfused with PBS and dissociated into a single cell solution in 10mM HEPES RPMI containing 0.2% collagenase, 0.1mg/mL Dispase II, and 2000U/mL DNaseI. Cells were passed through a 70um strainer and stained at 4 °C for 30 minutes with following antibodies (1:100), SiglecF-APC, MHCII-APC-Cy7, CD11c-PE. Cells were sorted with BD FACSAria2 or SONY SH800 FACS. mRNA is isolated from total RNA using Dynabead mRNA purification kit. cDNA was prepared using the NuGen Ovation RNAseq system V2 kit, Sequencing libraries were generated using Nextera XT library preparation kit.
GEO Accession
GSM3037383