GSM2358603: miR-155; Homo sapiens; ChIP-Seq
The cell lysates were incubated with 5 μg of anti-DIG antibody (Roche). Precipitated DNA was purified and subjected to next generation sequencing (NGS) on an Illumina instrument. The experiment was repeated using biotin-labeled miRNAs for transfection into Akata cells and PureProteomeä Streptavidin Magnetic Beads (Millipore) for immuno-precipitation. Immuno-precipitated DNA was purified and subjected to NGS. The TruSeq® ChIP Library Preparation Kit (Illumina) was used for library construction. The procedure was following the standard protocol provided by Illumina.
GEO Accession
GSM2358603