GSM2358909: Genistein_Rat_3; Rattus norvegicus; MBD-Seq
The fourth abdominal mammary glands were rapidly dissected from live ketamine/xylazine anesthetized animals (to minimize proteolysis), snap-frozen in liquid nitrogen, and stored at -80 ºC for later analysis. Methylated DNA was obtained by the MethylMiner Methylated DNA Enrichment Kit (Invitrogen). Eluted DNA was used to generate libraries following the standard protocols from Illumina. Next, MBDCap-seq libraries were sequenced using the Illumina Genome Analyzer II as per manufacturer's instructions. Image analysis and base calling were performed with the standard Illumina pipeline.
GEO Accession
GSM2358909