GSM2599673: SK-UT-1-FASN input; Homo sapiens; ChIP-Seq
Chromatin immunoprecipitation (ChIP) were performed on SK-UT-1-FASN and SK-UT-1-EV cells using the Magna ChIP A/G kit from (Millipore, Temecula, CA) following the manufacturer’s protocol. Anti-H3K9me3 antibody was used to immunoprecipitate cross-linked and fragmented chromatin DNA. This was followed by elution of protein/DNA complexes, reversal of the cross-links, and DNA purification. Library preparation and massively parallel DNA sequencing (ChIP-seq) was performed with eluted DNA using an Illumina Hiseq 2500 sequencer by the City of Hope Integrative Genomics Core (Duarte, CA).
GEO Accession
GSM2599673