C57BL/6 EAE stool 16s 3
16S V4 amplicons were generated according to 16S Metagenomic Sequencing Library Preparation Guide provided by Illumina Technologies. Next, dual indices and Illumina sequencing adapters were added to amplicons using the Nextera XT Index Kit. Libraries were pooled, denatured and spiked with PhiX internal control prior to being run on the Illumina MiSeq system using MiSeq Reagent Kit v3 (Illumina).