GSM4020394: WT_totalRNA_M_rep1; Drosophila melanogaster; RNA-Seq
3rd instar stationary larvae were dissected in a magnetic chamber and all internal tissues were removed. The larval carcasses were put in in lysis buffer and frozen. Total RNA was extracted with the RNAeasy Mini Kit (Qiagen). 1ug of RNA was treated with 20 units of RNAseR (Epicentre) in a 25uL reaction at 37oC for 30 minutes. Mock samples were treated with the same volume of water.  All samples were then cleaned up with 2 volumes of RNA Clean XP beads(Agencourt) and eluted with 13uL of water. 10uL of mock and RNAseR treated samples were used to make RNA libraries using the TruSeq Stranded total RNAseq with Ribozero gold LT kit (Illumina). Libraries were pooled in equimolar proportions and sequenced on a 2x75bp High-output NextSEq500 cartridge