GSM4201942: 8-DplusP; Drosophila melanogaster; Homo sapiens; ChIP-Seq
Cells were crossed-linked with formaldehylde solution. Then nuclear extracts were isolated, and sonicated. Chromatin bound to phospho Ser2 Pol II was isolated using RNA polymerase II (phospho CTD Ser-2), clone 3E10, rat monoclonal, and chromatin bound to total Pol II was isolated uisng RNA polymerase II, clone CTD4H8, mouse monoclonal. ChIP-Seq libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, E7645). Fragments between 200 and 500 base pairs were selected using a Pippin Prep 1.5% agarose cassette (Sage Science, Beverly MA US), pooled and 75 single-end base pair reads were sequenced using the Illumina NEXTseq 500.   NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, E7645)
GEO Accession
GSM4201942