GSM3335817: mid_siTRPS1_2_RepliSeq; Homo sapiens; ChIP-Seq
For ChIP-seq, the crosslinked protein-DNA complex was extracted from the sonicated nuclei and isolated with antibody. For Repli-seq, EdU incorporated nascent DNA was sonicated and enriched with streptavidin magnetic beads. For extrachromosomal DNA sequencing, extrachromosomal DNA was purified from the nuclei treated with ATP-dependent exonuclease to remove linear genomic DNA. Enriched DNA for ChIP-seq, Repli-seq or extrachromosomal DNA sequencing was end polished with T4 DNA polymerase and kinase. An 'A' base was added to the polished DNA fragments, followed by the Qiaquick column clean up. Sequencing adaptors were ligated to the DNA fragments and enriched by 15 cycles of PCR amplification. 200-300 bp size fractions were selectively isolated from the 1% agarose gel and eluted by the Qiagen gel extraction kit. The extracted DNA was quantified by Qubit 2.0 and subjected to HiSeq X Ten or HiSeq 2500 sequencing according to the manufacturer's instructions.
GEO Accession
GSM3335817