GSM2360493: SAM24295048 mRPTEC 6hrs TWEAK; Mus musculus; RNA-Seq
RNA was prepared using RNEasy micro (Qiagen) Total RNA (RNA integrity number >5; 250 ng) was put into the Illumina TruSeq RNA Sample Preparation Kit v2 protocol and run in conjunction with Biomek liquid handling platforms (Beckman Coulter Life Sciences, Brea, CA). RNA libraries were evaluated using the Agilent 2200 TapeStation HighSensitivity D1K Tape and quantitative polymerase chain reaction (qPCR) with the KAPA Library Quantification Kit for Illumina sequencing. Then 2 nM of library (12 samples per lane) was loaded for cluster generation and sequenced on the Illumina HiSeq 2000 Sequencing System at 250 base pairs read length plus index read. Reads that passed quality filters were deter- mined, and fastq files were generated by Illumina CASAVA v1.8
GEO Accession
GSM2360493