GSM2358959: p53NULL_shPFKFB4_rep2; Homo sapiens; RNA-Seq
Total RNA from 3 tumours in each treatment group was extracted using RNeasy mini columns (Qiagen) with on column DNase I digestion. PolyA+ RNA was extracted using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, Ipswich, MA, USA). For library preparation, the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA) was used. Quantification and quality control was performed using the Experion Automated Electrophoresis System (BioRad, Hercules, CA, USA). Sequencing was performed using an Illumina NextSeq500 System (Illumina, San Diego, CA, USA).
GEO Accession
GSM2358959