Seasonal variation of picocyanobacterial diversity in the ECS and ES
To elucidate picocyanobacterial ITS sequence diversity in the East China Sea and the East Sea, barcoded amplicon pyrosequencing with GS-FLX Titanium (454 Life Sciences, USA) was conducted. To amplify partial picocyanobacterial ITS sequences, barcoded primers were used. The barcoded primers consisted of adapter A (forward) or B (reverse)   Key (TCAG)   barcode (10-mers, forward only)   picocyanobacteria-specific primers. The ITS-af (5’-GGA TCA CCT CCT AAC AGG GAG-3’) and ITS-ar (5’-GGA CCT CAC CCT TAT CAG GG-3’) primers were used as specific primers.
Location of station H6
33.00N, 127.5E
Location of station I
32.115N, 125.179E
Location of station D5
37.08N, 131.73E