Rhodococcus erythropolis ACN1 with acetic acid (replicate #3)
Rhodococcus erythropolis ACN1 was grown in a baffled flask containing 50 ml M9 medium with 25 mM acetic acid and ammonia as sole carbon and nitrogen source at 25 C and 180 rpm. Cells were harvested at an OD600 of approx. 1.0 by centrifugation at 10000 g for 1 min. RNA isolation was done with the Rneasy Mini Kit (Qiagen).