RNA-Seq of Limnoria tripunctata: hepatopancreas
Samples were prepared from hepatopancreases by dissecting 50 animals, which were collected in TRIzol Reagent (Thermo Fisher Scientific) on ice, ground with a micropestle and total RNA extracted according to the manufacturers protocol. Total RNA was treated with RQ1 DNase (Promega) to remove genomic DNA, cleaned with RNA Clean & Concentrator-5 (Zymo Research), then quantified spectrophotometrically (NanoDrop 2000, Thermo Scientific) and its integrity evaluated using the Bioanalyzer2100 (Agilent). The transcriptomic library was constructed after poly-A selection using the TruSeq Stranded mRNA Sample Preparation Kit (Illumina) according to the library protocol.