16S rRNA gene sequencing of peanut spermosphere
High quality DNA samples were used for bacterial 16S rRNA gene amplification by Beijing BIOMARKER (Beijing, China). The specific primers 338F (forward primer, 5'- ACTCCTACGGGAGGCAGCA-3') and 806R (reverse primer, 5'- GGACTACHVGGGTWTCTAAT-3') with barcode were used for bacterial 16S rRNA tags (V3 and V4 region) amplification.