Amplicon-seq of CRISPR cut site, unmodified control, rep1
Total DNA was extracted from infected cells with Qiagen DNeasy kit.     A 470 bp PCR product surrounding the UL23 cut site was amplified using Phusion     high-fidelity polymerase (NEB) and column purified (Macherey-Nagel). Primers     contained a 5-overhang compatible with Illumina NGS library preparation. Amplicons     were pooled and sequenced on an Illumina Miseq (2x300 paired-end). Library     preparation and sequencing was performed by SeqMatic (Fremont, CA,     USA).