GSM4144939: C4_1_1; Mus musculus; RNA-Seq
mRNA from T cells is isolated by using Trizol reagent. All samples need to pass through the following three steps before library construction: (1) Nanodrop: for RNA purity (OD260/OD280). (2) Agarose gel electrophoresis: for RNA integrity and potential contamination. (3) Agilent 2100: check RNA integrity again. 1 µg of RNA was used for cDNA library constuction at Novogene using an NEBNext@ Utra RNA library Prep Kit for Illumina@ (cat# E7420S, New England Biolabs, lpswich, MA, USA) according to the manufacturer's protocol.
GEO Accession
GSM4144939