GSM1982298: iNKT 1; Homo sapiens; RNA-Seq
mRNA extraction was performed using RNeasy micro kit (QIAGEN) according to standard instructions. Sample1-13: cDNA synthesis was performed following to Ovation RNA-seq2 (NuGEN), after which the Illumina library was prepared using Encore NGS Multiplex System I (NuGEN). The libraries were sequenced using a HiSeq 2500 in 101 cycle Single-Read mode. Sample14-17: cDNA was synthesized using SMARTer Ultra Low Input RNA for Illumina Sequencing-HV kit (Clontech), after which the Illumina library was prepared using a Low Input Library Prep kit (Clontech). The libraries were sequenced using a HiSeq 2500 in 101 cycle Single-Read mode.
GEO Accession
GSM1982298