GSM2087959: Thermus at MARS5 24 hours, biological rep 2; Thermus scotoductus SA-01; RNA-Seq
Total RNA was extracted with the RNeasy Mini Kit followed the manufacturer’s protocol with the following modification.  180 µL of lysozyme (15 mg/mL) re-suspended in TE Buffer (30 mM Tris-HCl, 1 mM EDTA, pH 8) plus 20 µL Proteinase K (Qiagen) was added to each cell pellet and samples incubated with agitation (125 rpm) for 25 min at room temperature.  The optional on-column DNase digestion was extended to 1 hr. Ribosomal RNA were depleted from RNA samples using Ribo-Zero rRNA Removal Kit for Bacteria (Illumina, San Diego, CA) according to manufacture recommend protocol prior to RNA library preparation.  RNA library preparation was carried out using NEBNext® Ultra™ RNA Library Prep Kit for Illumina (New England Biolabs, Inc., Ipswich, MA) according to manufacture recommend protocol.
GEO Accession
GSM2087959