Illumina HiSeq 2000 sequencing; LMNB1_A_IN_CAWDWACXX_lane7
Protocols :  Extraction Protocol: XRNAX extracts were fragmented through ultrasonication (Covaris) and used for immunoprecipitation (IP) or size-matched input control (SMI-control). For the SMI-control 5 % of the sample were end-repaired with FastAP and PNK treatment. For the IP, protein-RNA complexes were immunoprecipitated and captured on magnetic beads before end-repair occurred on-bead. Both IP and SMI-control were resolved by SDS-PAGE, blotted on nitrocellulose, areas corresponding to the molecular weight of the protein-of-interest+75kDA excised an digested with proteinase K. RNA was purified with PCI extraction and isopropanol precipitation. For a detailed version of this protocol and technical advice visit www.xrnax.com/applications. Library Protocol: Small RNA sequencing library preparation with randomized adapters (UMIs). For XRNAX-CLIP-seq gel-based size selection for fragments between 30-50 nt was performed as described by the manufacturer's instructions. Multiplexing occurred so that 12 samples could be combined in one lane.
Factor Value[Antibody]
none
Factor Value[Treatment Compound]
na
Factor Value[Treatment Time]
na