Illumina HiSeq 3000 sequencing; ChIP-sequencing of 3xFLAG-AntA from Streptomyces albus S4
ChIP-sequencing of 3xFLAG-AntA from Streptomyces albus S4
Streptomyces albus S4 strains were grown in Lennox Broth (Kieser et al. Practical Streptomyces Genetics. The John Innes Foundation. Norwich, United Kingdom) whilst shaking for 48 hours at 28 degrees Celsius. Next, a final concentration of 1% formaldehyde was added to 38.94 ml of mycelium and was mixed on a rotary platform for 30 degrees Celsius for 30 minutes. Formaldehyde cross-linking was quenched by adding 2.7 ml of 2M glycine, followed by incubation at room temperature for 5 minutes. Mycelia was collected by centrifugation and washed twice with 40 ml ice cold PBS prior to lysis. P-MTAB-51958 P-MTAB-51959 P-MTAB-51956
Experimental Factor: genotype
antA/attB C31::pAU3-45-3xFLAG-antA-1