GSM3107830: Wildtype_RNAseq_Rep1; Saccharomyces cerevisiae; RNA-Seq
Cells were rapidly sedimented and snap-frozen on dry ice. Whole cell extracts were generated by breaking the cells with glass beads. Detailed information available in the Methods section of the article. cDNA libraries were prepared using Illumina ScriptSeq Complete Gold Kit for yeast (BGY1306) and ScriptSeq Index PCR Primers set 1 (RSBC10948) according to the manufacturers protocol with the following adjustments, in order to avoid depletion of short RNAs including snoRNAs. Purification of the rRNA-depleted samples was performed by ethanol precipitation instead of AMPure bead purification. cDNA was purified by the MineElute PCR Purification kit (Qiagen) in place of the AgencourtAMPure XP system
GEO Accession
GSM3107830