GSM2358098: piPSC-T; Homo sapiens; Bisulfite-Seq
Genomic DNAs were extracted from reprogramming cells and iPSCs using DNeasy Blood & Tissue Kits (QIAGEN). Genomic DNAs was digested by MspI(Fermentas,ER0541),the digested DNA was then end-repaired and tailed with an extra A to the 3'blunted ends.Illumina standard premethylated indexed adaptors were ligated with the dA-tailed DNA fragments. Bisulfite conversion was then performed using the MethyCode bisulfite conversion kit (Invitrogen,MECOV-50).Libraries were prepared according to KAPA DNA Library Preparation Kits.
GEO Accession
GSM2358098