GSM3336273: EHMT2_ChIPSeq; Homo sapiens; ChIP-Seq
Cells were lysed with Farnham lysis buffer followed by RIPA buffer, Chromatin sheared and DNA was extracted as per manufacturer's protocol ChIP kit (#17-295, Mercmillipore, Bedford, MA, USA). 30ng ChIPed DNA and corresponding input were used for ChIP-seq library construction (New England Biolabs). Library fragment size was determined using the DNA 1000 Kit on the Agilent Bioanalyzer (Agilent Technologies). Libraries were pooled in equimolar and cluster generation was performed on the Illumina cBOT system. Sequencing (150bp pair-end) was performed on the Illumina MiSeq according to manufacturer's protocol at the Duke-NUS Genome Biology Facility, Singapore. Sequencing reads were mapped against hg19 and G9a bound regions were identified by MACS2.
GEO Accession
GSM3336273