GSM1982258: A549_m6A_IP2; Homo sapiens; OTHER
mRNA was first purified from total RNA using PolyATtract® mRNA Isolation Systems (Promega). Then 5 g mRNA was fragmented and immunoprecipated with anti-m6A antibody (Synaptic Systems, 202003), the immunoprecipated RNA was washed and eluted by competition with N6-Methyladenosine (Sigma-Aldrich, M2780). The purified RNA fragments from m6A meRIP were used for library construction using TruSeq Stranded mRNA Sample Prep Kits (Illumina RS-122-2101) Library was constructed using TruSeq Stranded mRNA Sample Prep Kits (Illumina RS-122-2101) according to the manufacturer’s instructions.
GEO Accession
GSM1982258