GSM2598423: RPE1 cell siRNA Control 24h RNA-seq library; Homo sapiens; RNA-Seq
Total RNA was extracted from the cells transfected with either siRNA at 24h and 48h time points and were prepared with RNeasy Mini kit (Qiagen) and treated with ribo-Zero kit (illumina) to remove ribosomal RNA. A single replicate was performed per condition. RNA-seq libraries were prepared with a NEBNext® Ultra™ RNA Library Prep Kit for Illumina (NEB), according to manufacturer’s instructions and sample uniquely barcoded using NEBNext Multiplex oligo for Illumina (NEB). Samples were quantified using a BioAnalyzer 2100 with a High Sensitivity DNA Kit (Agilent). RNA-seq libraries were analysed by Edinburgh Genomics at the University of Edinburgh, UK, who performed quality control and sequencing using an Illumina HiSeq2500, 50 base single-end reads. Samples were indexed as follows: q1_RPE1_siRNA_con_24h NebNext illumina index2, q2_RPE1_siRNA_SAFA_24h NebNext illumina index4, q3_RPE1_siRNA_con_48h NebNext illumina index5 and q4_RPE1_siRNA_SAFA_48h NebNext illumina index6.
GEO Accession
GSM2598423