GSM3401783: Control-3; Mus musculus; RNA-Seq
About 50 to 100 mg of tumor samples were homogenized in 1 ml of TRIZOL reagent supplied with 200l of stainless steel beads and 0.2 ml of chloroform was added to the samples. Samples were vortexed vigorously for 15 seconds and then incubated at room temperature for 2 to 3 minutes. After centrifugation at 12,000 x g for 15 minutes at 4°C, upper aqueous phase of the samples was carefully removed to a new tube and the total RNA was purified by RNeasy Mini Kit (Qiagen) according to the manufacturer's instruction. cDNA libraries were constructed using the Ion AmpliSeq Kit for proton sequencer(Thermo Fisher) with Ion AmpliSeq Custom Panel that was designed by the manufacturer (Thermo Fisher) using Ion AmpliSeq Designer for targeted amplification of 4,604 mouse genes Ion AmpliSeq Custom Panel
GEO Accession
GSM3401783