GSM3504520: TG43; Mus musculus; RNA-Seq
Microglia were isolated using CD11b MicroBeads methods. Total RNA were isolated and sent to Cofactor Genomics (St. Louis, USA) for RNA deep sequencing. RNA samples were first examined using Agilent Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA) for purity and quality. RNAs were reverse transcribed to cDNA using Ovation RNA-Seq System Version 2 (NuGEN, San Carlos, CA) according to the manufacturer's protocol. The resulting cDNAs were then sheared using a focused ultrasonicator (Covaris Inc., Woburn, MA, USA) and the libraries were prepared using the Kapa LTP Library Preparation Kit (Illumina, San Diego, CA, USA)
GEO Accession
GSM3504520