GSM1102665: Colo829_MNase-seq; Homo sapiens; MNase-Seq
Cells are lysed in 1% SDS lysis buffer and sonicated for ChIP analysis. RNA extraction and amplification from preimplantation embryos and ICM were carried out as described before (Xie et al., 2010). For ChIP-seq, the libraries were prepared with Illumina's 'TruSeq DNAseq Sample Prep kit'. The libraries were then pooled and quantitated by qPCR. Each pool each was sequenced on one lane for 101 cycles on a HiSeq2000 using a TruSeq SBS sequencing kit version 3. For RNA-seq of ICM and blastocyst, there are two-round of RNA Amplification. The amplified RNAs were subjected to sequencing using Illumina’s small sample RNA-seq protocol.
GEO Accession
GSM1102665