RNASeq of Desulfurivibrio alkaliphilus: Sulfide oxidation - Replicate 2
Cultures of Desulfurivibrio alkaliphilus were grown under sulfide oxidizing conditions. Total RNA was extracted from three biological replicates using the MO BIO PowerSoil RNA Isolation kit followed by purification and DNA digestion  using the ZymoResearch RNA concentrator kit 5. Ribosomal RNA was depleted using the Ribo-zero rRNA depletion kit (illumina). The sequencing library was constructed as an Illumina TruSeq strand-specific mRNA library. Libraries were sequenced as SR50 reads on an Illumina HiSeq 2500 sequencing platform.