Genome sequencing of Pseudomonas sp. strain ICMP 22404
Total DNA from strain ICMP 22404 was extracted from a liquid culture grown overnight using a DNeasy Blood and Tissue DNA purification kit (Qiagen, USA) according to the manufacturers instructions. One microgram of DNA was randomly fragmented by sonication and a library was constructed using a NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs, UK) following the manufacturers instructions. The resulting library was sequenced on an Illumina HiSeq platform using a 2 x 150 bp paired-end configuration, generating a total of 22 million raw reads.