GSM3036733: cKO PRDM16 ChIP-seq replicate1; Mus musculus; ChIP-Seq
PRDM16 ChIP-seq was carried out using dual chromatin crosslinking with PFA and EGS, followed by sonication and antibody precipitation. H3K27ac and H3K4me ChIP-seq were carried out using native chromatin digestion with microccocal nuclease, followed by antibody precipitation of DNA-histone complexes. For library preparation, genomic DNA was purified, end repaired, ligated with barcoded adaptors, amplified for 11 PCR cycles and purified using the Ovation Ultralow System V2 (NuGEN). Library fragments in the range of 100-800 bp were size-selected using agarose gel electrophoresis followed by DNA gel extraction and purification.
GEO Accession
GSM3036733