GSM3881036: S. pneumoniae, SpSL1, 90 Minutes; Streptococcus pneumoniae; RNA-Seq
10 ml of pneumococcal cells were added to phenol (5%)/ethanol (95%) and pelleted at 4000 rpm at 4 degrees. For RNA extraction pellets were resuspended in 50 μl TE (pH8) with 3 mg/ml lysozyme and incubated at 37°C for 20 mins to lyse cells. The Maxwell 16 LEV simplyRNA Cells kit (Promega) was then used along with a Maxwell 16 LEV instrument (Promega) for RNA extraction. RNA samples were processed with the ScriptSeq Complete Kit for bacteria (CamBio) RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM3881036