GSM3039264: Wild-type replicate 2; Mus musculus; RNA-Seq
Renal cortex was collected from one harvested kidney and minced with a scalpel then pushed through a 70 micron sieve followed by digestion with collagenase IV for 30 mins at 37 C. Cells were pelleted by centrifugation for 3 mins at 300g, followed by two washes with PBS. Cell suspensions underwent drop-seq according to the protocol of Macosko et al (PMID: 26000488). Cell barcode is the first 12 nt of read 1. UMI is position 13 to 20 of read 1.
GEO Accession
GSM3039264