GSM4088914: 4T1 Cell Line Brca1 KO bulk RNA-seq Rep 5; Mus musculus; RNA-Seq
For cells lines , Qiagen RNAeasy kit was used for isolation RNA libraries were prepared for sequencing using standard Illumina protocols For mouse single cell analysis, orthotopic implanted tumors in the mammary fat pad were isolated at day 12 after implantation. Single cell suspensions were generated from 2 pooled tumors using the Mouse Tumor Dissociation Kit according to manufacturer instructions (Miltenyi Biotech). Singe cell suspensions were then stained with DAPI and antibodies to CD45 and live lymphoctyes (DAPI-CD45+) cells were sorted on a BD Aria cell sorter (gating strategy below). Single cell library preparation was performed according to manufacturer instructions using the mouse single cell RNAseq kit (10X genomics) with 10,000 cells input per sample. Three replicates of each condition (4T1 Parental, 4T1 Brca2null , and  4T1 Brca1null tumors) were processed although 1 Brca1null  sample failed initially quality control and was excluded.
GEO Accession
GSM4088914