GSM3262154: RNA-seq_βTC3_r1; Mus musculus; RNA-Seq
Total RNA was prepared using the Qiagen RNeasy mini kit (Qiagen) with an on-column DNase I digestion using the Qiagen RNase-Free DNase set as the manufactures guidelines Ribosomal RNA was depleted using the RiboMinus Eukaryote Kit for RNA-Seq (Life Technology) and RiboMinus Concentration module as manufactures' guidelines. Sequencing libraries where prepared using NEBNext mRNA Library Prep Master Mix Set (NEB), following the manufactures protocol, with all size selection performed using Agencourt AMPure XP beads (Beckman Cutler). Samples where multiplexed using the NEBNext Multiplex Oligos for Illumina kit to barcode each sample and amplified for 11 cycles. Sequnced using an Illumina Hi-Seq 2000 single end 50bp reads
GEO Accession
GSM3262154