DaCBF4 overexpressor, normal control, biological replicate #2
Total RNAs were extracted from at least 5 plants of each genotype and each treatment using Qiagen Plant RNeasy extraction kit (Qiagen) and RNase free DNase I (Qiagen, Germany). Three different biological replicates were prepared. The integrity and concentration of RNA was determined using Bioanalyzer (RIN>6) and Qubit   RNA BR assay kit (Life Technologies, USA). To construct the sequencing library, 1.5   g of total RNA of each sample was used as input for the TruSeq RNA sample prep kit v2 (Illumina, USA).