15q Duplication Syndrome iPS-derived neuron RNA-Seq
Total RNA was isolated using Trizol. Strand-specific RNA-seq libraries were prepared using prerelease Directional mRNA-seq Library Kits (Illumina). Briefly, poly(A)+ RNA was isolated by oligo-dT selection, fragmented, and treated with phosphatase and polynucleotide kinase to repair the ends. RNA adapters (3' and 5') were then sequentially ligated to the RNA fragments and reverse transcribed using a primer complementary to the 3' linker. The libraries were then PCR amplified and sequenced on either an Illumina GAIIx using paired-end 76 bp chemistry or a HiSeq2000 using paired-end 100 bp reads.