Genome Sequence Reads of KS720(SAMN02928179)
Individual V. cholerae strains from a human cholera patient stool sample were colony purified, and then each was grown up in broth culture for genomic DNA (gDNA) purification using the Qiagen DNeasy Blood & Tissue Kit. The gDNA was then prepared for single-end, 50 base-pair sequencing on the Illumina HiSeq2500 using the HTML-PCR method (Biotechniques 54:25-34, 2013).