Illumina MiSeq paired end sequencing; Time-lapse Deep-seq of E. coli Metabolite Production Pathway
Time-lapse Deep-seq of E. coli Metabolite Production Pathway
Upon each new seed, a time-series sample (2 mL culture) was collected and stored at -20 deg. C. according to detailed specifications in manuscript Growth in 2xYT medium according to manuscript specifications. As described in the manuscript, in EVO2 five parallel lineages (c6-c10) of E coli equipped with pMevT were cultured by serial passing. Each new seed (2 % volume) was conducted following 8 hours of cultivation to avoid stationary-phase culture. For EVO10, different versions of the pathway were also studied (lineages c2-c4 and c6-c8) according to detailed description in the manuscript, but following the same overall growth setup Nucleic acids extracted by standard plasmid extraction kit (Macharey Nagel) Nextera XT v2 with two extra limited PCR cycles
Experimental Factor: seed (8-hour time point)
6
Experimental Factor: individual
EVO2c8