GSM2598457: d15_LPS_Sham_2_merged_*.fastq; Mus musculus; RNA-Seq
Total RNA was isolated using the miRNeasy kit (Qiagen) according to the manufacturer’s instructions from 8 individual mouse cervices per timepoint (day 15 gestation, day 15 sham, day 15 LPS, and day 18 gestation) for 2 biological replicate libraries (4 cervices each) at each timepoint. The total RNA was then enriched for polyA+ RNA using Dynabeads Oligo(dT)25 (Invitrogen).  The polyA+ RNA was then used to generate strand-specific RNA-seq libraries as described previously (Zhong et al., 2011).  The RNA-seq libraries were subjected to QC analyses (i.e., number of PCR cycles required to amplify each library, the final library yield, and the size distribution of final library DNA fragments) and sequenced using an Illumina HiSeq 2000
GEO Accession
GSM2598457