GSM2176563: CC2; Brassica oleracea; RNA-Seq
Using TRIzol reagent (Invitrogen, Life Technologies) according the manufacturer’s protocol, total RNA was extracted from two biological replicates (three plants per replicate) for each genotype. NanoDrop ND 1000 (NanoDrop technologies) was used to initially calculate the quality and quantity of the extracted RNA, and then the RNA Integrity Number (RIN) value was assessed by Agilent Technologies 2100 Bioanalyzer (Agilent). Only when the value of RIN was higher than 8, the RNA was used to prepare the c-DNA library according to the TruSeq RNA Sample Prep v2 protocol
GEO Accession
GSM2176563