RNA-seq of  homo sapiens: Lung Adenocarcinoma Cell Line
Total RNA was isolated by TRIzol. RNA quality was determined byAgilent 2100 Bioanalyzer (RIN > 8), and quantity was measured by Qubit fluorometer. 1 g RNA was then prepared with the TruSeq Stranded Total RNA LT Sample Prep Kit from Illumina. Poly-A RNA (mRNAseq) is purified and fragmented before strand specific cDNA synthesis.cDNA are then a-tailed and indexed adapters are ligated. After adapter ligation, samples are PCR amplified and purified with Ampure XP beads, then run on the Illumina NextSeq 500/550 system (Kits V2.5) with 75 bp single end reads to product about 25 Million reads per sample.