WGS of Guzerá:Guz1
One mate-paired library with 3-4 kb insert, with both forward and reverse tags, were sequenced to 50 bases for 2 x 50 bp. Mate-paired libraries were prepared according to the Applied Biosystems SOLiD 4 System Library Preparation Guide [Applied Biosystems, CA, USA]. In total, 60 ľg of genomic DNA were sheared using HydroShear systemTM [DigiLab Genomic Solutions Inc., Mass., USA] into random fragments. After shearing, the DNA fragments were end-repaired and linked to adapters. The linked DNA fragments were then fractionated by electrophoresis in 1% agarose gel and fragments of sizes between 275 and 350 bp were cut from the gel and purified. As a last step, the library was PCR amplified and purified using PureLink PCR Purification Kit and quantified with Qubit fluorometer 2.0 (Invitrogen) using the High Sensitivity Test dsDNA. Emulsion PCR reactions were performed using SOLiD EZ Bead Systems [Life Technologies Corporation, CA, USA] following the recommendations of the manufacturer.