GSM4201911: 2-WT-D2; Homo sapiens; RNA-Seq
RNA was extracted using Trizol/Chloroform extraction, DTT was added to protect 4sU to be converted. RNA pellet was treated with IAA to convert the 4sU, then cleaned up using ethanol. 3' RNA-sequencing, sequencing libraries were prepared from 500ng RNA using the Lexogen QuantSeq 3' mRNA Seq Library Prep Kit FWD for Illumina. 75 single-end base-pair reads were sequenced using the Illumina NEXTseq 500.  QuantSeq 3' mRNA Seq Library Prep Kit FWD for Illumina
GEO Accession
GSM4201911