GSM3770748: Daoy_ PELP1 shRNA_2; Homo sapiens; RNA-Seq
Daoy cells transduced with lentiviral particles expressing either control shRNA or PELP1 shRNA vector and total RNA was isolated using RNeasy mini kit using manufacturer's protocol (Qiagen, Valencia, CA). Total RNA samples about 1-2 micro grams were used for sequencing library preparation according to Illumina TruSeq Total RNA Sample Preparation Guide (Illumina Cat. #: RS-122-2201). Each library was bar-coded and then pooled for cluster generation and sequencing run with 50bp single-end (SE) sequencing protocol.
GEO Accession
GSM3770748