GSM4201901: MiRNA-Seq liver TSC1 mutant, replicate 5; Mus musculus; miRNA-Seq
Total RNA was isolated from 50mg of mouse liver and was homogenized in 1ml TRIzol (Sigma) using lysing matrix D tubes (Q-Biogene) and a bead beater. Subsequent steps were performed as per TRIzol manufacturer's instructions. 1ug of total RNA was used to generate small RNA libraries for sequencing as described in R. Gumienny et al.,Nucleic Acids Res 45, 2341-2353 (2017). Libraries were sequenced on an Illumina HiSeq-2500 deep sequencer.
GEO Accession
GSM4201901