454 sequencing of AMF: subtropical forest plant roots: Sample D_12
"454 sequencing of AMF in plant roots in subtropical forest. For direct 454 unidirectional sequencing of the fungal 18S gene amplicons, forward 454 primer was constructed to combine a 454 sequencing adaptor (A adaptor), a 10-base tag and NS31 primer (Simon et al., 1992); backward 454 primer was constructed to combine a DNA capture bead anneal adaptor (B adaptor, for emulsion PCR) and AML2 (Lee et al., 2008). Amplifications were performed in a Gene Amplification PCR System. The PCR product was sent to company for 454 sequencing using FLX Titanium"