GSM3403494: epi42; Arabidopsis thaliana; OTHER
Approximately 10 seedlings were harvested per epiRIL for ~100mg tissue from which DNA was extracted using the Qiagen DNeasy Plant Mini Kit, following the manufacturer's instructions (Qiagen N.V., Hilden, Germany). Before library preparation, DNA was fragmented to an average insert size of 350bp using 24 cycles of 30 seconds each on a Bioruptor® Diagenode sonication device. The genomic DNA sequencing libraries for the Arabidopsis epiRILs were prepared using the TruSeq DNA PCR-Free LT Library Prep Kit following the manufacturer's instructions (Illumina, San Diego, CA), starting from  1.1µg of sonicated DNA. Libraries were validated using the High Sensitivity D1000 screentape on the 2200 Tapestation instrument (Agilent technologies, Santa Clara, CA) and the LightCycler® 480 Instrument II using the LightCycler® 480 SYBR Green I Master mix (Roche, Basel, Switzerland).
GEO Accession
GSM3403494