GSM2176425: mESC_LG_rep2; Mus musculus; RNA-Seq
RNA was extracted using Trizol reagent and purified with RNAeasy columns (Qiagen). RNA was reverse transcribed with the high capacity cDNA kit (Applied Biosystems). NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, NEB) was used to isolate mRNA derived from 2 µg total RNA. Sequencing libraries were prepared using the NEBNext® mRNA Library Prep Reagent Set for Illumina® (NEB). Libraries were quantified on MultiNA capillary electrophoresis system (Shimadzu Biotech) and sequenced at a concentration of 12 pM on the Genome Analyzer IIx (Illumina Inc,).
GEO Accession
GSM2176425