GSM3504406: DT003_S3; Mus musculus; RNA-Seq
Females (4 diphtheria toxin treated and 4 PBS1X) were scarified at day E8 of pregnancy and the entire uterus (with fetal tissues) was collected and samples were incubated overnight in RNAlater (Qiagen) at 4°C and then transferred to -80°C for storage. Samples were then lysed and homogenized using the tissue lyser (Qiagen) and total RNA was purified using Trizol (Invitrogen) according to the manufacturer's instructions. RNA yield was assessed using a NanoDrop 1000 spectrophotometer (NanoDrop Products, Thermo Fisher Scientific). RNA integrity was assessed using an Agilent Bioanalyzer showing a quality RNA integrity number of 8–10 (Agilent Technologies). The RNA was processed using the Illumina TotalPrep RNA Amplification Kit Protocol according to the manufacturer's protocol. High quality libraries were prepared using the Kapa mRNA hyperprep kit (ROCHE). The quality control was done in Tapestation and Quantifluor. Illumina sequencing of the 8 samples in 100 million reads/sample was done with the NextSeq 500 High Output Kit v2 (300 cycles) sequencer (Illumina Inc) generating paired end reads of approximately 2 x 75 bp in length.
GEO Accession
GSM3504406