GSM2439380: Scrambled 2 rep 3; Mus musculus; RNA-Seq
Mice were euthanized with pentobarbital for P6 or with isoflurane for P14. Thoracic cavity was opened and lungs were perfused through the heart with PBS. Lung were placed in RNAlater (Qiagen) overnight in 4°C and then transferred to -80°C. Lungs were homogenized with Precellys 24 homogenizer. Total RNA was extracted with peqGOLD Total RNA kit following the manufacturer’s instructions. Library preparation was performed with the TruSeq Stranded mRNA HT technology High Sample (HS) according to the manufacturer´s protocol
GEO Accession
GSM2439380