GSM2598509: WT_3; Mus musculus; RNA-Seq
Reverse transcription was carried out directly on the whole oocyte lysate using the SMARTer Ultra Low Input RNA for Illumina Sequencing - HV kits (Takara Bio Inc., Kusatsu, Japan) according to the manufacturer’s instructions. The resulting first strand cDNA was purified and then amplified for 17 cycles by PCR. After amplification, the cDNA was purified and subjected to library construction for deep sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM2598509