GSM3504591: 12A T8 r3; Homo sapiens; RNA-Seq
RNA was isolated at 8 time points: T1: 0 mins (confluent proliferating myoblasts); T2: 440 mins (54-6 alignment), T3: 530 mins (54-12 alignment), T4: 1355 mins (54-6 initiation of fusion), T5: 1505 mins (54-12 initiation of fusion), T6: 1860 mins (54-6 myotube formation), T7: 2165 mins (54-12 myotube formation) and T8: 5040 mins (myotube maturation) using miRNeasy kit (Qiagen, Manchester, UK) including DNase digestion from each cell line in triplicate. RNA quality/concentration were checked by LabChip Bioanalyzer (RIN>8.0) and Nanodrop. RNA-seq libraries were prepared using the Agilent sureselect stranded RNAseq protocol, which allows polyA selection but was modified to work with ribodepletion. Libraries were sequenced on an Illumina HiSeq2500.
GEO Accession
GSM3504591