RNA-Seq of TCCSUPPi replicate 1
Total RNA was extracted using Qiagen RNeasy Micro Kit (Qiagen, Germany) following the manufacturers instructions.  Genomic DNA was removed from the RNA samples by DNase Max treatment (Cat no.: 15200-50, MO Bio Inc.). RNA quality and RNA integrity number (RIN) were assessed by  spectrophotometer(NanoDrop 2000) and Bioanalyzer 21000 system (Agilent Technologies, Santa Clara, CA, USA). RNA quantification was performed using Qubit RNA Assay Kit and Qubit 2.0 Fluorometer (Life Technologies, San Diego, CA, USA).  Transcriptome library synthesis was provided by Malaysia Genome Institute (MGI), Bangi, Malaysia, based on TruSeq Stranded mRNA library preparation kit v2 (Illumina, San Diego, CA) starting at the fragmentation step using a total of 2 g RNA per sample.