E. crypticus transcriptome sequencing using 454 GS FLX titanium
The transcriptomic library was constructed by isolating total RNA from animals from different life stages and exposed to a number of chemical and physical stressors. Total RNA from the different isolates was pooled, transcribed to cDNA and normalized to compensate for high abundant transcripts. The normalized cDNA pool was sequenced using 454 GS FLX titanium sequencing in a full 2 gasket microtiterplate.