GSM3960853: Neutrophils, Control 6; Homo sapiens; RNA-Seq
Neutrophils were isolated by percoll gradient and 1-3 x 106 freshly purified neutrophils were suspended in RNAlater (RLT) lysis buffer. From this lysate RNA was purified using the QIAamp RNA Blood Mini Kit (Qiagen, Hilden, Germany). cDNA was synthesized with SuperScript™ VILO™ cDNA Synthesis Kit (Thermofisher Scientific, Waltham, Massachusetts, USA) in an Ampliseq plate from the Ion AmpliSeq™ Kit for Chef DL8 (Thermofisher Scientific). The Ion AmpliSeq™ Transcriptome Human Gene Expression Panel, Chef Ready Kit (Thermofisher Scientific) was used to produce amplicons from 20,000 RefSeq genesLibrary preparation was done on the Ion Chef sytem (Thermofisher Scientific) . Libraries were sequenced on the Genestudio S5 (Thermofisher Scientific). Targeted RNA-seq
GEO Accession
GSM3960853