GSM2439360: porcine epiblast day 10-11 embryos rep1; Sus scrofa; RNA-Seq
iPSC RNA was isolated with RNAeasy micro kits from Qiagen and embryonic RNA was isolated using TriZol and the PureLink™ RNA Micro Kit (Invitrogen) iPSC: After the total RNA extraction and DNase I treatment, magnetic beads with Oligo (dT) are used to isolate mRNA(for eukaryotes) or by removing rRNAs from the total RNA (for prokaryotes).  Mixed with the fragmentation buffer, the mRNA is fragmented into short fragments. Then cDNA is synthesized using the mRNA fragments as templates. Short fragments are purified and resolved with EB buffer for end reparation and single nucleotide A (adenine) addition. After that, the short fragments are connected with adapters. The suitable fragments are selected for the PCR amplification as templates. embryonic material: RiboZero kit (EpiBio, Medison USA) was used to deplete for ribosomal RNA, and hexamers for priming the reverse transcription, followed by regular sample preparation for sequencing.
GEO Accession
GSM2439360