GSM1440510: Floret LN_rep1; Zea mays; RNA-Seq
The cob (the central white tissue of the ear) and florets (the peripheral light yellow tissue of the ear) of maize ear were harvested separately, flash frozen and stored at -80℃. Total RNA was extracted from cob and florets with TRIZOL reagent (Invitrogen). 6 µg of total RNA from each sample was used in library construction according to the standard DGE protocol. Briefly, mRNA was purified by oligo(dT) magnetic beads adsorption (Invitrogen) and reverse transcribed into cDNAs. The cDNAs were digested with an anchoring restriction enzyme (NlaIII or DpnII) and ligated to an Illumina-specific adapter, Adapter 1, containing a recognition site for the type IIS restriction enzyme MmeI. Following MmeI digestion, cDNAs were purified and a second Illumina adapter, Adapter 2, was ligated to the 3' ends. After 15 cycles of linear PCR amplification, 95bp fragments were purified by 6% TBE PAGE Gel electrophoresis and then sequenced by an Illumina HiSeq 2000 sequencer for 49 cycles.
GEO Accession
GSM1440510