Illumina HiSeq 2000 sequencing; RNA-seq of blood and adipose tissue from mice infected with Trypanosoma brucei
RNA-seq of blood and adipose tissue from mice infected with Trypanosoma brucei
Mice infected for four days were sacrificed and blood collected by heart punctures. Trypanosoma brucei parasites were purified over a DEAE column. Total RNA was extracted using TRIzol LS reagent according to the manufacturers instructions (Life Technologies).  Ribosomal RNA was depleted with RiboMinus kit (Invitrogen). Total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturers instructions (Invitrogen) and cDNA was synthesized using random hexamers according to Complete RNA-seq Library System kit (NuGEN) instructions.
Experimental Factor: blood
organism part