GSM2948570: WT => St2-/- biological replicate 3; Mus musculus; RNA-Seq
Total RNA was extracted from snap-frozen tumors collected from the colon of  bone marrow chimeras using Trizol®Reagent (Life Technologies). Extracted RNA was then further purified using RNeasy Plus Mini Kit (Qiagen) according to the manufacturer's instructions. Libraries were generated using the Illumina Truseq RNA sample preparation kit according to the manufacturer's instructions. Three biological replicates were generated and sequenced for each group of BM chimeras by subjecting 1μg of RNA to single-end (50 pb) sequencing on an Illumina HiSeq 2500 instrument at the Australian Genome Research Facility.
GEO Accession
GSM2948570