GSM4706335: aorta adventitia Cd45+ at steady state; Mus musculus; RNA-Seq
After sorting CD45-positive immune cells from whole aortas, viable cells were proceeded for single cell capture using the Chromium Controller (10X Genomics). Barcoding and library preparation was done using Chromium Next GEM single cell 3' (v3.1, 10x Genomics). The resulting 3' Gene Expression libraries were sequenced on an Illumina HiSeq 1500 (Illumina, San Diego, USA) in paired-end mode with asymmetric read length of 28+91 bp and a single indexing read of 8bp, to reach approximately 50.000 reads per single-cell. Single cell RNA sequencing
GEO Accession
GSM4706335