GSM2178967: S oligo 2; Rattus norvegicus; RNA-Seq
GH4C1 cell were fixed with 1% paraformaldehyde in PBS. Then nuclei were purified, lysed and sonicated. RNA pull-down was performed using two antisense DNA biotinylated oligonucleotide probes that target the lncRNA Neat1. Streptavidin-magnetic beads were added to hybridization reaction and complexes were captured by magnets. RNA was isolated using NucleoSpin®RNA XS (Macherey-Nagel). Libraries were prepared according to instructions accompanying the Illumina TruSeq Stranded mRNA Sample Preparation kit.
GEO Accession
GSM2178967