GSM2089289: HY.ARH_GABA_NPY-hrGFP rep6; Mus musculus; RNA-Seq
Acutely extracted brain was sliced at 300um thickness, digested with pronase for 1 hour at RT, triturated and GFP labeled cells were sorted manually. RNA was extracted using PicoPure kit (ThermoFisher, KIT0204). Extracted RNA was amplified using Ovation RNA-Seq System V2 (NuGEN, #7102). 1~1.5 ug of the amplified material was used for library construction using Encore NGS Multiplex System (NuGEN, #0314). adapters: truseq
GEO Accession
GSM2089289