RNA-Seq of Rattus norvegicus: ISIAH strain, 3 months, hypothalamus
All samples were stored in RNA Later (Qiagen, Chatsworth, CA) at -70C until use. mRNA was extracted using Dynabeads mRNA Purification Kit (Ambion, USA). cDNA libraries were constructed using NEBNext mRNA Library Prep Reagent Set for Illumina (NEB, USA) following the manufacturer's protocol and were subjected to single-end sequencing on Illumina HiSeq1500 platform.