RNA-Sequencing of Bos taurus
Total RNA extraction and DNase I treatment was done using  miRNeasy Micro Kit (217084, Qiagen). RNA libraries were prepared by using 1  ng of total RNA per sample following the low input workflow of SMART-Seq  Stranded Kit (Takara Bio, Inc.). followed by deep sequencing using the  Illumina NextSeq 500 platform. The workflow used in this kit takes advantage  of an innovative technology allowing removal of ribosomal cDNA (cDNA  fragments originating from rRNA molecules) after cDNA synthesis using probes  specific to mammalian rRNA. The rRNA depletion method used in this kit makes  it especially well-suited for working with very small quantities of total RNA  or cells, including intact single cells. SMART-Seq Stranded Kit (Takara Bio,  Inc.) provides additional benefits,  including strand-of-origin information and a fuller representation of the  transcriptome. Indeed, initiation of reverse transcription using random  priming instead of the oligo(dT) priming for sequencing leads to the capture  of the full transcriptome instead of only the polyadenylated fraction.