GSM3108342: G2_RNA-seq; Rattus norvegicus; RNA-Seq
The bone around the implant was dissected with a margin of 1 mm and the surrounding muscle tissues were thoroughly removed.Then the remaining bone specimens were grinded into powder while submerged in liquid nitrogen and total RNA was isolated with QIAGEN miRNeasy Mini Kit (QIAGEN, USA). Approximately 3 µg of total RNA were used to construct cDNA library. Briefly, the sample was first subjected to ribosomal RNA depletion through Ribo-Zero rRNA Removal Kit (Human/ Mouse/Rat) (Epicentre, USA) according to the manufacturer's protocol and then fragmented in Fragmentation Buffer (Thermo Fisher Scientific, USA). The resultant RNA fragments were reverse-transcribed to construct the cDNA library via the previously described dUTP method[23]. The amount of library DNA and the size distribution of the inserted fragments were determined by Qubit 2.0 RNA Broad Range Assay Kit (Invitrogen, USA) and on a 2100 Bioanalyzer (Agilent, USA), respectively. Library was subsequently sequenced on a HiSeq 4000 platform (Illumina, USA) that produced 150-bp paired-end reads.
GEO Accession
GSM3108342