GSM3771333: A549_TGFB_nutin_p53cs_r2; Homo sapiens; ChIP-Seq
ChIPseq lysates were clarified from sonicated nuclei and IPed with DO-1 anti-p53  antibody ( DO-1, Santa Cruz Biotechnology, SC-126X, Lot:G0115).   RNA samples were extracted with Trizol, purified on Zymo columns, and 500ng polyA selected. DNA and mRNA libraries were prepared with standard illumina compatible protocols (NEBnext Kit)