Genome sequencing of Nannochloropsis oceanica: isolate KB1
1 ug of gDNA was used for library preparation, which were constructed using the Ligation Sequencing Kit (LSK-109, Oxford Nanopore Technologies), in a reaction modified to use half volumes of LSK-109 reagents. Recommended concentration were used again, following library pooling. Barcodes BC01 and BC02 were used for Nannochloropsis oceanica BR2 and KB1, respectively. 13 cycles of PCR were used, following ONT specifications with a 12 minute extension step. The resultant pooled and barcoded library was sequenced using an ONT MinION sequencer on a R9.4 flow-cell and basecalled using Guppy v3.1.5 with default settings.