RNA-Seq of mus musculus spinal cord
sample was snap frozen within 1-2 min post-mortem. Sample was pre-incubated at -70C in RNAlater-ICE (Ambion) for 24hrs before total RNA was extracted with Qiagen RNeasy Plus micro kit. Libraries were created using TruSeq stranded mRNA library kits and sequenced on an Illumina HiSeq 4000 to create 2x75bp paired end RNA-seq samples.