GSM3959505: shCtrl-rep2-MeRIP; Homo sapiens; OTHER
120 ug of purified total RNA was determined with NanoDrop ND-1000. Intact mRNA was isolated from total RNA samples using Arraystar Seq-StarTM poly (A) mRNA Isolation Kit (Arrarystar, MD, USA) following the manufacturer's instructions, and was chemically fragmented into 100-nucleotide-long fragments by incubation in fragmentation buffer. Fragmented mRNA was immunoprecipitated with anti-N6-methyladenosine (m6A) antibody (Synaptic Systems, Goettingen, Germany), and also 1/10 of the fragmented mRNA was kept as input. RNA-seq libraries were prepared using KAPA Stranded mRNA-seq Kit (Illumina, CA, USA). The completed libraries, qualified by Agilent 2100 Bioanalyzer, were denatured and diluted to loading volume of a loading concentration. Clustered libraries were loaded onto reagent cartridge and forwarded to sequencing run on Illumina Hiseq 4000 system by Aksomics (Shanghai, China). MeRIP-seq
GEO Accession
GSM3959505