GSM3639736: mpm control3 rep 2; Burkholderia thailandensis; RNA-Seq
Bacterial pelets were treated with1,000 U lysozyme for 15min at 37◦C prior to dissolving cells in TRIzol reagent, and total RNA isolated using the miRNeasy Mini kit (QIAGEN). Total RNA was depleted of ribosomal RNA using the Ribo-Zero Gram-Negative Bacteria kit (Illumina, Cat. #MRZGN126). cDNA libraries were generated with ScriptSeq v2 Library Preparation Kit (Illumina, Cat. #SSV21106), titrated with the Qubit dsDNA HS Assay (ThermoFisher Scientific, Cat. #Q32854), and  sequenced on the Illumina Mi-Seq platform, generating paired-end 151 bp reads
GEO Accession
GSM3639736