PFLA_10X
DNA was extracted using a phenol/chloroform protocol and gDNA quality and purity was assessed using spectrophotometry, fluorometry and capillary electrophoresis. 10X Chromium Library was prepared according to 10X Genomics protocols from 10ng of gDNA using the Genome Reagent Kits v2.  Library quality was assessed using capillary electrophoresis and quantified by QPCR using the Kapa Library Quantification Kit. Sequencing was performed on an Illumina HiSeq3000 using a 2x150 pb paired-end read length.