Illumina HiSeq 2000 sequencing; 4_WT_D4_C8M0LACXX_lane5
Protocols :  Extraction Protocol: RNAs were extracted from 1 Mio. cells using RNeasy Kit (Qiagen), followed by DNase digestion using TURBO DNase (Thermo Fisher). RNA was quantified using Nanodrop and RIN score quality assessment was determine using Bioanalyzer. PolyA-mRNA was isolated from 1 microg of total RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) before sequencing library preparation. Library Protocol: mRNAs were isolated from 1 microg of total RNA using a PolyA selection kit (NEB) and sequencing libraries were prepared following instructions from NEBs Ultra Library Preparation Kit for Illumina. All samples were barcoded, amplified in 12x PCR cycles, pooled and sequenced on a HiSeq2500 Sequencer (Illumina) using a 50 bp single-end run.
Factor Value[Cell Type]
Embryoid Bodies
Factor Value[Dev. Stage]
Day four
Factor Value[Genetic Modification Type]
none
Factor Value[Genotype]
Wild Type