GSM2439554: cow-milk-fed_pig_rep3; Sus scrofa; miRNA-Seq
The total RNA were extracted using the Qiagen miRNeasy® Mini Kit (Qiagen, Hilden, Germany) Extracted total serum RNA from the two different animal models was concentrated in a speedvac to a final volume of 10 µl. From this 5µl was used as input in the libraries, which were prepared accordingly with TruSeq Small RNA Sample Preparation kit (Illumina). There was made one modification to the Illumina protocol, since we increased the number of PCR cycles from 11 to 16 for total RNA with a 1 µg as starting material. Additionally, we applied TruSeq SR Cluster Kit v3 for cluster generation and furthermore for sequencing TruSeq SBS Kit v3 was applied.
GEO Accession
GSM2439554