SCS_S9_800m
The bacterial hypervariable regions V1-V3 of the 16S rRNA genes were amplified using primers 27F (5?-AGAGTTTGATCCTGGCTCAG-3?) and 534R (5?-ATTACCGCGGCTGCTGG-3?) with a unique 10 bp error-correcting Golay barcode at the 5? end. Equimolar amounts of the PCR amplicons from different samples were mixed and purified.