DNA-seq of homo sapiens:  female breast cancer
A custom panel covering the coding DNA sequence of the 106 genes that are known to be altered in at least 1% of the HRPBC cases was designed with SureSelect technology, and an Illumina HiSeq2000 device was used. The depth was set to a minimum of 500X to enable studying very low minor allele fractions (MAFs) and their changes.