GSM3446325: ubH2B CHIP-seq in USP22 wild type (shLuc), Thaps treated cells, replicate 1; Homo sapiens; ChIP-Seq
Cells were fixed in 1% formaldehyde for 10 minutes at room temperature, followed by quenching with 0.18 M Glycine for 5 minutes. Cells were harvested and chromatin was extracted 10 ng of DNA from Inputs and IPs were used to prepare libraries using Accel-NGS 2S Plus DNA Library Kit (Swift Biosciences, MI) following manufacturer's protocol with an additional wash at the end using SPRIselect beads (Beckman Coulter, IN) to remove any larger libraries that could interfere with clustering on the instrument
GEO Accession
GSM3446325