GSM3261859: WT5_LPS_24; Homo sapiens; RNA-Seq
RNA was extracted using RNeasy Plus Mini kit (Qiagen). RNA integrity was assessed by Bioananalyzer 2100 (Agilent) and RIN>9,2 were obtained for all samples. RNA was diluted to 20 ng/ul and used as input to create mRNA libraries using TruSeq Stranded mRNA kit (Illumina, CA, USA) with dual indexing following standard instructions. Libraries were validated on the Fragment Analyzer platform (AATI, IA, USA) using standard sensitivity NGS fragment analysis kit and the concentration was determined using Quant-iT dsDNA High Sensitivity assay kit on the Qubit fluorometer (Thermo Fisher, MA, USA). Sample libraries were pooled in equimolar concentrations and diluted and denatured according to Illumina guidelines. Sequencing was performed using a High Output 2 x 76 bp kit on an Illumina NextSeq500.
GEO Accession
GSM3261859