GSM2598231: ∆spxA2 H2O2 1; Streptococcus mutans UA159; RNA-Seq
Total RNA was isolated from homogenized S. mutans cell lysates by hot acid-phenol:chloroform extractions.  RNA was precipitated with ice-cold isopropanol and 3 M sodium acetate (pH 5) at 4°C before RNA pellets were dissolved in nuclease-free H2O and treated with DNase I.  For each total RNA extract, 10 μg RNA aliquots were subjected to a second DNase I treatment using the DNA-free kit (Ambion). The TruSeq RNA Sample Preparation Kit V2 (Illumina, San Diego, CA) was used for next generation sequencing library construction following the instructions from the manufacturer. Single end reads of 100nt were generated for each sample and aligned to the organism specific reference genome.
GEO Accession
GSM2598231