GSM3108125: McSC_TyrTom; Mus musculus; RNA-Seq
1) Back skin of Tyr-CreER; R26R-Tomato mice were incubated with 0.25% Trypsin at 37 degree for 2 hrs and then stirred in DMEM+10% FBS for 30min to get single cells, which were FACSed to isolated Tomato+ single melanocyte stem cells.  2) Melanoma tumors were chopped and incubated in 0.35% collagenase I for 1hr at 37 degree (100rpm shaking) to get single cells, which were FACSed to get single Tomato+ tumor cells. Single-cell RNA-Seq libraries were prepared using the following Single Cell 3' Reagent Kits v2: Chromium™ Single Cell 3' Library & Gel Bead Kit v2, PN-120237; Single Cell 3' Chip Kit v2 PN-120236 and i7 Multiplex Kit PN-120262" (10x Genomics) as described in https://www.ncbi.nlm.nih.gov/pubmed/28091601, and following the Single Cell 3' Reagent Kits v2 User Guide (Manual Part # CG00052 Rev A).
GEO Accession
GSM3108125