GSM3769182: NB4_24h_dmso_2; Homo sapiens; RNA-Seq
Cells were harvested at the indicated timepoints and RNA was extracted using RNEasy Mini Kit (Qiagen) with on-column DNAse digestion. The RNA was further concentrated and purified with RNEasy Micro kit (Qiagen). RNAseq libraries were prepared from 33 NB4 APL cell line samples using the Illumina Tru-Seq Stranded Total RNA kit protocol, according to the manufacturer's instructions (Illumina, San Diego, California, USA). Library fragment size was determined using the DNA 1000 Kit on the Agilent Bioanalyzer (Agilent Technologies). Libraries were quantified by qPCR using the KAPA Library Quantification Kit (KAPA Biosystems).  Libraries were pooled in equimolar and cluster generation was performed on the Illumina cBOT system (Illumina). Sequencing (150bp pair-end) was performed on the Illumina HiSeq 3000 system at the Duke-NUS Genome Biology Facility (Singapore), according to manufacturer's protocol (Illumina).
GEO Accession
GSM3769182