Illumina HiSeq 2500 paired end sequencing; Transcriptome analysis of Beijerinckiaceae RH AL1 under methanol oxidation conditions
Transcriptome analysis of Beijerinckiaceae RH AL1 under methanol oxidation conditions
Biomass was collected by centrifugation (10000 x g, 10 min, at room temperature). Pelleted biomass was flashfrozen and kept at -80 °C before being processed. Beijerinckiaceae bacterium RHAL1 was grown to exponential phase in minimal medium containing methanol as carbon source (1 % [v/v]) and lanthanum as growth supplement. Total RNA was extracted following a protocol modified after Wegner et al., 2013 (doi: 10.1016/j.margen.2012.12.001). Total RNA was enriched for mRNA by rRNA depletion using the MICROBExpress   bacterial mRNA enrichment kit (Thermo Fisher Scientific, Schwerte, Germany).  RNA-Seq libraries were prepared using the NEBNext Ultra II directional RNA library prep kit for Illumina (New England Biolabs, Frankfurt, Germany).
Experimental Factor: replicate
1