Full-length transcriptome sequencing of zebrafish (Danio rerio) : adult female unfertilized eggs.
RNA sample Z1-M was reverse transcribed into cDNA. Four fragment ranges (0.5-1K, 1-2K, 2-3K, and >3K) were prepared according to the PacBio Iso-Seq protocol. PacBio RS II sequencing reactions of 16 SMRT (single-molecule real-time) cells (3 cells of 0.5-1K, 5 cells of 1-2K, 5 cells of 2-3K, 3 cells of >3K) were performed in DNA Sequencing Reagent 4.0 (Clontech, Mountain View, California, USA).