Whole genome sequencing of Wolbachia strain wCer2 from DsimRC45
Wolbachia was extracted from the Drosophila simulans host. This involved the enrichment of Wolbachia cells from 15 to 30 dechorionated embryos through selective centrifugation and filtration, thus isolating Wolbachia cells from host chromosomes and tissue. DNA from Wolbachia cells was amplified by multiple displacement using the Qiagen Repli-g Midi kit, then cleaned-up using the Qiagen DNA Mini kit. The library  was prepared with TruSeq PCR-free (350bp insert) library kit (Illumina), using 1 g of input DNA, and the paired-end (2 x 125bp) library was sequenced on the Illumina HiSeq 2500 platform.