RNA-Seq of Solanum lycopersicum: odorless-2 leaflet
Samples were prepared with the Illumina TruSeq Stranded mRNA Library Preparation Kit. Libraries were validated and quantified using a combination of Qubit dsDNA assay, Caliper LabChipGX and Kapa Illumina Library Quantification qPCR kit. Library concentrations were normalized and pooled in equimolar amounts. The pool was loaded on one lane of an Illumina HiSeq 2500 High Output flow cell (v4) and sequencing was carried out in a 2x125bp paired end format using HiSeq SBS reagents. Base calling was done by Illumina Real Time Analysis (RTA) v1.18.64 and output of RTA was demultiplexed and converted to FastQ format with Illumina Bcl2fastq v1.8.4