GSM3960914: LN_WIV+CD8a ALN-1, replicate 1; Mus musculus; RNA-Seq
CD8+ T cells were sorted out (4°C) of the mixed cell populations and captured in 350 mL of RLT+ lysis buffer, supplemented with b-mercaptoethanol (4°C) (RNeasy Plus Micro Kit, 74034, Qiagen). The concentration, quality and integrity of RNA was addressed using an Agilent 2100 Bioanalyzer (Agilent) and only samples with an RNA integrity number (RIN) >=  8, 280/260- and 260/230-values > 1.8 were sent for sequencing. The VIB Nucleomics Core performed the library preparation and library pooling using standard Illumina protocols (TruSeq kit).