GSM1162578: Pitx2-flag input; Mus musculus; ChIP-Seq
Chromatin immunoprecipitation was performed as described previously using EZ ChIPTM kit (Millipore) and Anti-FLAG M2 Affinity Gel (Sigma) For the construction of library for sequencing, 10-50 ng of purified ChIP DNA was end-repaired using Ion Plus Fragment Library Kit (Part no. 4471252, Life Technologies) and purified with two rounds of AMPure®  XP beads (Beckman Coulter, Brea, CA) capture to size select fragments approximately 100–250 bp in length. The end-repaired DNA was ligated to Ion Torrent compatible adapters. Followed by 15 cycles of PCR amplification before downstream template preparation. DNA fragments of the completed library ranged from approximately 170–220 bp in length.
GEO Accession
GSM1162578