454 GS Junior sequencing of SAMD00068682
DNA was fragmented by digesting with dsDNA Fragmentase (New England Biolabs). The fragmented DNAs were purified using the MinElute PCR Purification kit (Qiagen). Two shotgun library were prepared using the NEBNext Quick DNA Library Prep Master Mix Set for 454 (New England Biolabs). Each fragmented DNA was end repaired, A-tailed, and ligated to one of the 12 multiplex identifier (MID) oligonucleotide adaptors (Roche) for multiplexed sequencing. For each shotgun library, small DNA fragments were subsequently removed using the AMPure purification system (Agencourt Bioscience).For one shotgun library, we performed target capture of microsatellite-containing DNA fragments by using the SeqCap EZ hybridization and wash kit (Roche), according to the general guidelines provided in the NimbleGen SeqCap EZ Library LR User??????s Guide ver.1.0. (Roche) We mixed two types of library (genomic and micro satellite enrichment).