16S rDNA of bacterial community: drinking water treatment plant
16S rDNA gene fragments were amplified using forward primer 341F:5¡¯-CCCTACACGACGCTCTTCCGATCTG-3¡¯and reverse primer 805R:5'- GACTGGAGTTCCTTGGCACCCGAGAATTCCA-3'. The PCR profile was as follows:94 ¡æ for 3 min; 5 cycles of 94 ¡æ for 30 s, 45 ¡æ for 20 s, and 65 ¡æ for 30 s; 20 cycles of 94 ¡æ for 20 s, 55 ¡æ for 20 s, and 72 ¡æ for 30 s with a final extension at 72 ¡æ for 5 min. And all of the procedures were conducted at Sangon (Shanghai Sangon Biotech Co., Ltd, China).