GSM2599586: D218_C2_70; Homo sapiens; RNA-Seq
All procedures of cell loading, capture, and library preparations were performed following the Fluidigm user manual and as previously described (Chu et al., 2016). Full-length, single-cell cDNA libraries were harvested from the C1 chip and diluted to 0.1–0.3 ng/μl, fragmented, and amplified using the Nextera XT DNA Sample Preparation and Index Kits (Illumina). Libraries were multiplexed at 24-48 libraries per lane and single-end reads were sequenced (Illumina HiSeq 2500 system).
GEO Accession
GSM2599586