GSM1440773: PolyA Transcripts replicate 1 (Nitrogen starvation 8 hr); Chlamydomonas reinhardtii; RNA-Seq
Cells were first lysed and total RNA extracted using Trizol and cleaned up using RNeasy column (Qiagen). PolyA+ RNA was isolated from 10ug of total RNA. RNA was fragmented using RNA Fragmentation Reagents (Ambion). Strand-specific RNA-seq library (Parkhomchuk et al., 2009) was constructed using Illumina DNA Sample Preparation Kit (Illumina) with 10 cycles PCR amplification.   * Parkhomchuk, D., Borodina, T., Amstislavskiy, V., Banaru, M., Hallen, L., Krobitsch, S., Lehrach, H., and Soldatov, A. (2009). Transcriptome analysis by strand-specific sequencing of complementary DNA. Nucleic acids research 37, e123.
GEO Accession
GSM1440773