LL1_Core_2.5
A pair of universal primers: 515F (5 GTG CCA GCM GCC GCG GTA A 3) and 806R (5 GGA CTA CHV GGG TWT CTA AT 3) (Caporaso et al. 2012, Itoh et al.2014, Pylro et al. 2014, Wu et al. 2015) was used to sequence the variable region V4 of the prokaryotic 16S rRNA gene for the detection of bacteria and archaea. Similarly, an another pair of universal primers: Euk7F (5 AAC CTG GTT GAT CCT GCC AGT 3) (Medlin et al. 1988, Auld et al. 2016) and Euk570R (5 GCT ATT GGA GCT GGA ATT AC 3) (Auld et al. 2016, Weekers et al. 1994) was used to sequence the variable region V1-V3 of the eukaryotic 18S rRNA gene to explore the eukaryotic microbial communities in the samples. The targeted variable regions of the 16S and 18S genes were sequenced on Illumina MiSeq platform (Caporaso et al. 2012, Garcia-Mazcorro et al. 2016)