GSM3109049: RNA-seq_WT_rep3; Arabidopsis thaliana; RNA-Seq
RNA was isolated using modified TRIzol method (Chomczynski and Sacchi 1987). Ribosomal RNA was removed using RiboMinus Plant Kit (Thermo Fisher) and ERCC RNA Spike-In Mix 1 (Thermo Fisher) was added. Libraries were prepaired with Ion Total RNA-Seq Kit v2 and Ion Xpress RNA-Seq Barcode 1-16 Kit according to user guide. Sequencing template was generated with Ion PI™ Template OT2 200 Kit v3 on Ion OneTouch™ 2 System. Sequencing was performed on Ion PI™ chip v2 and Ion Proton™ sequencer using Ion PI™ Sequencing 200 Kit v2 (all Ion Torrent kits and software are trademarks of Thermo Fisher).
GEO Accession
GSM3109049