Illumina HiSeq 2500 sequencing; RNA-seq analysis of PROX1 overexpression and knockdown in glioblastoma cell lines
RNA-seq analysis of PROX1 overexpression and knockdown in glioblastoma cell lines
Glioblastoma cell lines cultured in vitro PROX1 transduction in U-343 MG was performed using commercial lentiviral particles. Cells were selected using Puromycin.  PROX1 stable knockdown in cell line U-343-MGa was performed using lentiviral particles produced in house. Lentiviral vectors were transduced in HEK-293T cells using calcium-phosphate co-transfection. 24 hours after transfection supernantants were collected two times within 24-hour interval, filtered and then used to infect U-343 MGa cells. Cell were further selected with Puromycin antibiotic. Total RNA was purified using the PureLink® kit following the manufacturer's instructions (Thermo Fisher Scientific) Illumina TruSeq Stranded mRNA with Poly-A selection
Experimental Factor: phenotype
wild type phenotype
Experimental Factor: cell line
U-343_MGa