GSM3643587: SUM159-soft-2; Homo sapiens; RNA-Seq
Lysates were processed for RNA extraction using Isolate II RNA kit (Bioline), and 1µg from each sample was used for polyA selection with [Oligo d(T) Magnetic Beads,New England BioLabs #S1419S] For RNA-seq, cDNA was synthesized using the SuperScript III system from Invitrogen, followed by second strand synthesis, ds End Repair and UDG treatment to recover strand-specific libraries. Libraries were barcoded with BioO Nextflex adapters and amplified prior to sequencing
GEO Accession
GSM3643587