GSM4295047: AM324; Mus musculus; RNA-Seq
The hippocampus tissue samples of all female mice were collected into microcentrifuge tubes and weighed. Samples were homogenized in 250 µl of phosphate-buffered saline (DPBS, Lonza, Walkersville, MD, USA) using a stirrer. Fractions of homogenates were taken to RNA isolation (50 µl of homogenate and 500 µl Tri Reagent (Sigma-Aldrich, St. Louis, MO, USA) Library preparation and sequencing was conducted in Finnish Functional Genomics Centre (FFGC), Turku Centre for Biotechnology, University of Turku and Åbo Akademi University. The quality of the total RNA samples as well as prepared libraries was ensured with Advanced Analytical Fragment Analyzer. Sample and library concentration was measured with Qubit® Fluorometric Quantitation, Life Technologies. Library preparation was done according to Illumina TruSeq® Stranded mRNA Sample Preparation Guide (part # 15031047). The 45 libraries with good quality were pooled in one pool and run in 2 lanes. The samples sequenced using Illumina HiSeq 3000 instrument. Single-read sequencing with 1 x 50 bp read length was used, followed by 8 + 8 bp dual index run. 
GEO Accession
GSM4295047