Total RNA was extracted from sorted cells using Quick-RNA miniprep kit from Zymo Research (Cat# 11-328) Total RNA samples about 1-2 µg were used for sequencing library preparation according to Illumina TruSeq Total RNA Sample Preparation Guide (Illumina Cat. #: RS-122-2201). Each library was bar-coded and then pooled for cluster generation and sequencing run with 50bp single-end (SE) sequencing protocol.
GEO Accession
GSM3039938