Reverse transcription of viral RNA, followed by demi-hemi PCR of 4 amplicons with serotype-specific primers. Pooling of PCR products and creation of 454 libraries.
analysis_type
RNA Viral 454 Alignment and Assembly
gssr_id
42616.3
lsid
BROAD:SEQUENCING_SAMPLE:42616.3
material_type
Viral cDNA
project
V4642
work_request
22076