RNA was isolated from 0.5-1.0×10^6 cells either from subconfluent NPCs and GSCs (matched for density and media changes) or after 1 week removal of growth factors using TRIzol reagent (Thermo Fisher). G0/G1 cells were sorted on an FACSAria II (BD Biosciences) for the cells positive only for mCherry-hCDT1(aa30/120). Total RNA was prepared using Direct-zol™ RNA MiniPrep Plus Kit (ZymoResearch). RNA concentration and quality (RIN) were measured using an Agilent 4200 Tapestation. RNA libraries were prepared for sequencing using KAPA Stranded mRNA-Seq Kit (KAPA biosystems) according to manufacturer's guidelines.
GEO Accession
GSM3265735