Cells were collected and the pellets lysed in Trizol solution.  Extraction was performed using a Qiagen RNA preparation kit per standard protocol Libraries were constructed using purified total RNA as above.  Polyadenylated mRNA was purified, and the library for RNAseq was constructed using the Truseq v2 kit (Illumina).  Quality control was assessed by BioA analysis (Agilent Technologies) prior to library construction and again prior to sequencing
GEO Accession
GSM1446796