iCLIP experiments were performed following a published protocol (Konig et al, 2011), with minor modifications (Rodor et al, 2016). The immunoprecipitation (IP) step was carried out using an anti-RBM10 antibody (ab72423, Abcam) that recognizes the two isoforms of the murine RBM10 proteins. Four independent experiments of the RBM10 protocol were performed. The four RBM10 iCLIP libraries with different barcodes were pooled together and sequenced on a single lane by single end sequencing 50nt on an Illumina HiSeq 2000 system (BGI). Equivalent volume of the control libraries was sequenced on a different lane.
GEO Accession
GSM2363438