Total RNA was extracted using Trizol (Invitrogen) reagent from 10-day-old seedlings. sRNAs of 19-24 nt were gel-purified and ligated to a pair of adapters at the 5' and 3' ends by using T4 RNA ligase (NEB). sRNAs with adapters were transcribed into cDNA and amplified by Illumina sequencing-compatible primers.
GEO Accession
GSM3040118