ChIP-seq and input libraries were prepared following Illumina's protocol with some modifications. The precipitated DNA was blunted, phosphorylated, and ligated to single-end adapter dimers, and then amplified by PCR [30 sec at 98°C; (10 sec at 98°C, 30 sec at 65°C, 30 sec at 72°C) x 14 cycles; 5 min at 72°C]. Excess PCR primers and adapter dimers were removed using AMPure beads (Agencourt Biosciences Corporation). DNA library size selection was performed by excising the 250-350 bp region from a 2% agarose gel followed by purification using a QIAquick Gel Extraction Kit (Qiagen).
GEO Accession
GSM1446969