One hundred and fifty nanograms from (number of samples) RNA samples was used to generate libraries following Illumina TruSeq Stranded mRNA kit as per protocol. cDNA libraries generated from these samples were size indexed and validated using Agilent Bioanalyzer and concentration validated by qPCR.  4 different libraries were normalized to 10nM and pooled together. 8pM of pooled libraries were loaded onto Illumina cBot for cluster generation. Clustered flow cell was then sequenced Pair-end 100 cycles using Illumina HighSeq 2000.
GEO Accession
GSM2522990