This step was done by following the 10x genomics singel cell preparation protocol This step was done by following the 10x genomics single cell library preparation protocol Only the 3' end of mRNA fragements were used to make library. One end of the fragements were added P5, read1 illumina sequencing primer, 16 base pairs of 10x barcode, and UMI sequences; The other end ligated with P7, sample index sequences, and illumina read2 sequencing primer sequences.
GEO Accession
GSM3885058