Villus chromatin digested with MNase was incubated overnight with 5 ul H3K4me2 antibody (EMD Millipore, 07-030) for immunoprecipitation. ThruPLEX DNA-seq Kit (Rubicon Genomics) was used to prepare libraries, and 75 bp single-end reads were sequenced on an Illumina NextSeq 500 instrument.
GEO Accession
GSM2610647