C57BL/6 female mice (8 weeks old) were irradiated and transplanted with mouse HSPC transduced with a mTie2-IFN-mirT LV. At 20 weeks of age mice were sacrificed. Spleen tissue was dissociated and cells subjected to FACS sorting. Cd11b+ cells were recovered. C57BL/6 female mice (8 weeks old) were irradiated and transplanted with mouse HSPC transduced with a control mTie2-GFP-mirT LV. At 16 weeks of age mice were injected intravenously with 10-100 thousand B-ALL cells. At 20 weeks of age mice were sacrificed. Spleen tissue was dissociated and cells subjected to FACS sorting. Cd11b+ cells were recovered. C57BL/6 female mice (8 weeks old) were irradiated and transplanted with mouse HSPC transduced with a mTie2-IFN-mirT LV. At 16 weeks of age mice were injected intravenously with 10-100 thousand B-ALL cells. At 20 weeks of age mice were sacrificed. Spleen tissue was dissociated and cells subjected to FACS sorting. Cd11b+ cells were recovered. Droplet-based digital 3′ end scRNA-Seq was performed on a Chromium Single-Cell  Controller (10X Genomics, Pleasanton, CA) using the Chromium Single Cell 3′ Reagent Kit  v2 according to the manufacturer's instructions. Briefly, suspended single cells were  partitioned in Gel Beads in Emulsion (GEMs) and lysed, followed by RNA barcoding, reverse  transcription and PCR amplification (12-14 cycles). Sequencing-ready scRNA-Seq libraries were  prepared according to the manufacturer's instructions, checked and quantified on 2100  Bioanalyzer (Agilent Genomics, Santa Clara, CA) and Qubit 3.0 (Invitrogen, Carlsbad, CA)  instruments.
Experimental Factor: genotype
lentiviral expression of mTie2-GFP-mirT
Experimental Factor: stimulus
none
Experimental Factor: response to treatment
not applicable