Cells were scraped and collected and RNA harvested using TRIzol standard protocol. RNA was further purified using QIAGEN RNeasy kit. Intact poly(A) RNA was purified from total RNA samples (100-500 ng) with oligo(dT) magnetic beads and stranded mRNA sequencing libraries were prepared as described using the Illumina TruSeq Stranded mRNA Library Preparation Kit (RS-122-2101, RS-122-2102).
GEO Accession
GSM3263475