RNA was isolated using the HotPhenol method according to standard protocols. Prepared RNA was digested with DNaseI and cleaned up by phenol-chloroform extraction. Ribosomal RNA was depleted with the Ribo-Zero kit (Epicentre) for Gram-negative bacteria. Integrity of the prepared RNA was tested using a Bioanalyzer. Directional cDNA libraries were prepared using the NEBNext Ultra II Directional RNA Libary Prep Kit for Illumina (NEB E7760) according to the manufacturer's instructions. Quality of the prepared libraries was tested using a Bioanalyzer.
GEO Accession
GSM4456084