Extraction was performed with Qiagen's miRNeasy micro kit The quality of purified RNA samples was determined using a Bioanalyzer 2100 (Agilent).. The average RNA Integrity Number (RIN) was 8.9 and the minimum was 7.5 PolyAAA pulldown, amplification, and labeling was performed with the TruSeq Stranded mRNA kit.
GEO Accession
GSM4709867