1.5 million shControl, shChd1, shMap3k7 or shDouble WFU3 cells were plated onto 10cm dishes in complete growth medium. Twenty-four hours later RNA was isolated using 5prime Perfect RNA Cell kit (Fischer Scientific) according to the manufacturer's protocol. After quality control, RNA samples were enriched for mRNA using oligo(dT) beads, fragmented for a 250-300 bp target range, then cDNA synthesis using random hexamer primers, second strand synthesis is performed using dNTPs, RNAas H and DNA polymerase I in Illumina synthesis buffer, Illumina sequencing adapters, then the double-stranded cDNA library is size selected and PCR enriched.
GEO Accession
GSM3885208