Olfactory neuroepithelia were collected from three different WT and KO mice respectively. Total RNA was extracted with TRIzol Reagent (Life Technologies, Grand Island, NY). The quality of the RNA was determined with an Agilent 2100 BioAnalyzer. Equal amounts of total RNA (13 µg) were used for subsequent isolation of mRNA using Dynabeads mRNA purification kit (Invitrogen). Construction of RNA-seq libraries was done using NEXTflex Directional RNA-Seq kit (Bioo Scientific). cDNA library sequencing was performed with Illumina Hi-Seq 2500 at QB3 Vincent J. Coates Genomics Sequencing Laboratory in the UC Berkeley. library construction protocol
GEO Accession
GSM1827611