To isolate spores, 24 h fruiting bodies were collected, dissociated and sieved through 50 µm nylon mesh. The flow-through containing spores was passed next through 35 µm nylon mesh, and incubated with 0.1% Triton X-100 for 5 minutes to remove amoeboid cells. Dictyostelium lacteum amoebas were co-cultured with Klebsiella aerogenes on lactose-peptone agar. RNeasy kit (QIAGEN) was used for extraction. Libraries were prepared with Illumina Truseq stranded mRNA kit.
Experimental Factor: cell type
Spore
Experimental Factor: time
24