For ChIP-seq, the nuclei were sonicated in Sonication Buffer (20 mM Tris Cl pH 8.0, 2 mM EDTA, 150 mM NaCl, 1% NP40, 0.1% SDS, 1× PIC) using a Q800R3 Sonicator (Qsonica) with 65% amplitude for 40 minutes. Sheared DNA was incubated with 10 µg primary antibody (anti-HA, Cat#3724; anti-Flag, Cat#F1804; anti-H3K27ac, Cat#ab4729) at 4 °C overnight and then incubated with BSA-blocked Dynabeads Protein G for one hour. KAPA HyperPrep ChIP-seq library preparation kits (Roche, cat# 07962347001) were used for library construction.
GEO Accession
GSM4709601