Total RNA from embryoand larval samples was isolated using BioRad aurum kit.  Brain samples ware isolated following the method of Peña-Llopis and Brugarolas (2013). Library construction was done using Illumina TruSeq with Ribozero and 50 bp single end strand-specific sequencing was done at Tufts Molecular core facility.
GEO Accession
GSM2611128