RNAs were extracted with Qiagen RNeasy Plus Mini kit (Qiagen, Valencia, CA, USA). RNA integrity was assessed using the Agilent Bioanalyzer and samples had a quality score >8.0. RNA libraries were prepared using established protocol with NEBNExt Ultra II Directional Library Prep Kit for Illumina, (NEB, Lynn, MA). Each library was made with one of the TruSeq barcode index sequences and samples were sequenced across three lanes. The pools were clustered at 6.5 pM on a pair end read flow cell and sequenced for 300 cycles on an Illumina Hiseq 4000.
GEO Accession
GSM3778179