Total mRNA was extracted from cells using an RNA extraction kit (Qiagen K.K., Tokyo, Japan). RNA integrity was evaluated using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The samples with RNA.Integrity Number (RIN) ≥ 7 were subjected to the subsequent analysis. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM3656265