RNA was isolated from 1,000,000 cells  using the miRNeasy Mini Kit (Qiagen). Quality control and measurement of RNA concentration was done with a NanoDrop Spectrophotometer (Thermo Scientific, Wilmington, USA) and the Agilent 2100 Bioanalyzer (Agilent Technologies). Multiplexed library preparation of total RNA and deep sequencing with IlluminaHiSeq 2000 technology (IlluminaInc., San Diego, USA) with a read length of 50 bases was performed at EMBL gene core facility (Heidelberg, Germany).
GEO Accession
GSM1462859