Whole cell extracts from villi fixed with 1% formaldehyde were sonicated and incubated overnight with Dyna beads (Thermo Fisher Scientific) conjugated with 5 ug MED1 antibody (Bethyl Laboratories, A300-793A) for immunoprecipitation. ThruPLEX DNA-seq Kit (Rubicon Genomics) was used to prepare libraries, and 75 bp single-end reads were sequenced on an Illumina NextSeq 500 instrument.
GEO Accession
GSM2610639