Head and body tissues were separated and the digestive tract discarded. Samples were immediately stored in RNAlater Solution (Ambion) at 4°C for 24 hours. Immediately prior to extraction, pronotum, wings and legs were removed from the bodies; larvae were then dissected from the abdomen. Total RNA was extracted from remaining body tissue using RNeasy Plus kits with gDNA eliminator spin columns (Qiagen) according to the manufacturer's directions. Sequencing libraries enriched for mRNA were prepared using the TruSeq RNA Library Preparation Kit v2 (Illumina) at the University of Minnesota Genomics Center (UMGC; Minneapolis, MN).
GEO Accession
GSM4584956