Cells were harvested by trypsinization ESCs were cultured in N2B27 +2i/LIF Column purification For RRBS, genomic DNA was isolated using the QIAamp DNA Mini Kit (QIAGEN), after an overnight lysis and proteinase K treatment.  RRBS library preparation was performed as described previously (Boyle et al. 2012; doi.org/10.1186/gb-2012-13-10-r92), with the following differences: bisulfite treatment was performed using the EZ DNA Methylation-Goldâ„¢ Kit (Zymo Research Corporation) according to the manufacturer's protocol.
Experimental Factor: genotype
DPPA3 KO