RNA was prepared using RNAeasy Mini kit (Qiagen) as per kit protocol and with DNase treatment (Qiagen). Quality control was performed at the Dana Farber Cancer Institute’s Molecular Biology core. Technical duplicates with a minimum RIN score of 7 were processed. cDNA was synthesized using Clontech SmartSeq v4 reagents from 1 ng of RNA and fragmented to a mean size of 150bp with a Covaris M220 ultrasonicator. Illumina standard mRNA libraries were prepared from cDNA using Rubicon Genomics Thruplex DNaseq reagents according to manufacturer’s protocol
GEO Accession
GSM2602100