Total RNA was isolated using Trizol reagent (Invitrogen). mRNA was purified by using two rounds of purification with Dynabeads Oligo (dT)25 (Invitrogen) NEBNext Ultra directional Kit (NEB) was used omitting RNA fragmentation step for recovered m6A-RIP samples and following the manufacturer`s protocol for input samples.
GEO Accession
GSM2091064