Skin was removed, flash frozen in liquid nitrogen, and homogenized into a powder by mortar and pestle. RNA was extracted using Trizol reagent and the GE Healthcare Illustra RNAspin mini kit. RNA libraries were prepared for sequencing using standard Illumina protocols. Sequencing was performed by EA | Q² Solutions with Illumina HiSeq 2500 instruments.
GEO Accession
GSM4456009