RNA was purified using QIAGEN Micro RNEasy columns, and RNA quality assessed using Agilent Bioanalyzer 2 ng of total RNA was used as input for mRNA amplification using 5' template-switch PCR with the Clontech SMART-Seq v4 Ultra Low Input RNA kit, according to manufacturer's instructions. Amplified mRNA was fragmented and appended with dual indexed bar codes using Illumina NexteraXT DNA Library Prep kits
GEO Accession
GSM3265755