Total RNA was isolated using the TRIZOL reagent (Ambion, #15596018) according to manufacturer’s instructions. 10~100 ng total RNA from each embryo was processed for RNA-seq library preparation using the Ovation Mouse RNA-seq kit (NuGEN, #0348-32) followed the manufactory protocol, then massively parallel sequencing was performed with the Illumina HiSeq 4000 Sequencing System. 100-bp single-end reads were obtained per sample.
GEO Accession
GSM2442963