RNA were extracted by using Trizol agent (Invitrogen, Carlsbad, CA). RNA integrity was validated by using 2% agarose gel electrophoresis with Agilent 2100 Bioanalyzer, and RNA purity were verified based on absorbance (Abs) at 260 and 280 nm (Abs260/Abs280 > 1.8). cDNA library was constructed according to the standard protocol of NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (Invitrogen, Carlsbad, CA), and then, cDNAs were amplified by using polymerase chain reaction for 12 cycles. The amplified cDNA library was sequenced by utilizing Illumina HiSeq™ 2500 sequencer (Illumina, San Diego, CA, USA).
GEO Accession
GSM1830134