Cells were grown for 48 hours in 60 mm dishes, in their respective medium, at subconfluent density. Total RNA was extracted using TRIzol reagent (15596-018, Ambion life technologies) according to the manufactures protocol. Illumina TruSeq mRNA libraries were generated using the TruSeq RNA Library Preparation Kit v2 sample preparation kit (Illumina Inc., San Diego, Cat.No RS-122-2001/2) according to the manufacturer's instruction (Part # 15026495 Rev. B)
Experimental Factor: cell line
HCC1395