Total RNA was isolated using Trizol and Direct-zol RNA Miniprep kit (Zymo Reserach) according to the manufacturer's instructions. Total RNA samples about 1-2 mg were used for sequencing library preparation according to Illumina TruSeq stranded mRNA-seq Sample Preparation Guide. Each library was bar-coded and then pooled for cluster generation and sequencing run with 50bp single-end (SR) sequencing protocol.
GEO Accession
GSM3963324