Samples taken from the bioreactor (1 mL) were immediately harvested by centrifugation and the pellets flash frozen in liquid nitrogen Yeast cells were propagated using micro-bubbles for 10 h in a micro-bubble bioreactor. After 10 h, 10% of the culture used as inocula for regular bubble and micro-bubble oxygenated fermentations. During the fermentations, a gas mixture containing 1% O2 and 99% N2 was sparged to supply small amount of O2. Samples were taken for transcriptome analysis at the indicated times RNA isolation was performed by GENEWIZ PolyA selection and transcript library preparations were performed by GENEWIZ
Experimental Factor: growth condition
fermentation aerated with micro bubbles
Experimental Factor: time
4