Cyclohexamide was added (100mg/ul) to the cells and they were incubated for 5 minutes at 37 °C and then pelleted at 400g for 5 minutes. Media was removed and cell pellets were immediately snap frozen. Libraries were prepared according to McGlincy and Ingolia, Methods, 2017. Libraries were prepared according to McGlincy and Ingolia, Methods, 2017.
GEO Accession
GSM4647807