RNA was extracted with the GeneMatrix universal RNA purification kit (Roboklon). In vitro transcripts of rabbit b-globin, Firefly luciferase and Renilla luciferase were added, but later not used for normalization, because half-lives obtained by normalization to circRNAs matched those determined by metabolic labelling with 4-thiouridine much better. After depletion of rRNA, sequencing libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit by the Cell Networks Deep Sequencing Core Facility of Heidelberg University.
GEO Accession
GSM4697825