~100,000 hiPSC-derived NPCs were plated per well for bulk RNA sequencing samples. Prior to sample preparation, all equipment and surfaces were cleaned with RNaseZap (Sigma-Aldrich). Replicates of hiPSC-derived neurons were harvested at three different timepoints of differentiation (days 1, 3, and 7) with 200 µl Trizol (Invitrogen) per sample and stored at -80 °C until sequencing. CEL-seq2 protocol, followed by Single Cell Discoveries's adapted protocol for bulk samples barcoding all samples during RT with CEL-seq barcodes
GEO Accession
GSM4697469