Reverse transcription of viral RNA, followed by demi-hemi PCR of 4 amplicons with serotype-specific primers. Pooling of PCR products and creation of 454 libraries.
analysis_type
RNA Viral 454 Alignment and Assembly
gssr_id
34706.2
lsid
BROAD:SEQUENCING_SAMPLE:34706.2
material_type
Viral cDNA
project
V3149
work_request
22076