Embryos were homogenized and fixed with 1% PFA. Cells were lysed and nuclei purified and snap-frozen. 2*10^7 nuclei (Blastula) or 2*10^6 nuclei (preMBT) were pooled and fully lysed. Chromatin was sheared and DNA-Protein complexes were isolated with the respective antibodies and purified. ChIP-library was generated using the Illumina "ChIP-Seq DNA Sample Prep Kit" (Cat. No. IP-102-1001) according to the manufacturers instructions.
GEO Accession
GSM1168898