Total RNA was prepared using the QIAGEN RNeasy Plus Mini Kit Libraries were generated using the CLONTECH SMARTer HV kit, barcoding and sequencing primers were added using NexteraXT DNA kit.  Libraries were validated by microelectrophoresis, quantified, pooled and clustered on Illumina TruSeq v3 flowcell.  Clustered flowcell was sequenced on an Illumina HiSeq 2500 in 100-base single-read reactions.
GEO Accession
GSM3887812