20 ul of 0-4h embryos were used as starting material. Abcam anti-H3K27me3 antibodies (6002) were used for chromatin immunoprecipitation. Chromatin was sheared using BioRuptor instrument (Diagenode). DNA from precipitated material and input were used for library preparation using Illumina Nextera protocol.
GEO Accession
GSM2300785