Mice were euthanized and CD4 dendritic cells (CD11c+I-Ab+CD4+CD8-) were sorted from spleens on a FACSAriaIII (purity >99%). Equal number of DCs (5.0x105) in SRBC or PBS immunized group were then snap-frozen immediately for RNA isolation. Barcoded sequencing libraries were generated using 100ng of RNA with the Ovation RNA-seq System V2 and Encore Rapid Library System. RNA-seq for Illumina HiSeq 2500
GEO Accession
GSM1828773