Libraries were amplified by using overhang primers targeting the GGTAGTGGAGG and GG-GAGGTGGAGTAGG parts of the library and adding the Illumina TruSEQ adapter and universal or Index primers respectively. During amplification the extracted, TF-bound DNA was split in two separate pools and Illumina primer landing sites were added from both orientations respectively (Universal-for & Index-rev or Index-for and Universal-rev). Next, Illumina-specific barcodes were added in a 5 cycles PCR to allow for multiplexing of multiple samples. In order to obtain unidirectional reads starting from the barcode end of the library, reads need to be reverse-complemented and merged with the forward reads.
GEO Accession
GSM2607727