Approximately 50 berries were randomly selected from a 1000-berry biological replicate for RNA extraction. Total RNAs for RNA-seq analysis and real-time polymerase chain reaction (PCR) experiments were extracted from frozen deseeded berries using a plant RNA isolation kit (Sigma RT-250, St. Louis, MO, USA), and further incubated with DNase I (Promega, USA) according to the manufacturer’s instructions. RNA integrity and quality were assessed by analysis with the Aglient 2100 Bioanalyzer. The Gene Expression Sample Prep Kit (IlluminaInc; San Diego, CA, USA) was used for sequence tag preparation according to the manufacturer's protocol.
GEO Accession
GSM1828485