Genomic DNA was isolated from bulk tumour-bearing lung tissue from each mouse. Twelve benchmark control cell lines (3 cell lines of 500,000 cells each, 3 cell lines of 50,000 cells, 3 cell lines of 5,000 cells, and 3 cell lines of 500 cells) were added to each mouse lung sample prior to lysis to enable the calculation of the absolute number of neoplastic cells in each tumour from the number of sgID-BC reads. Q5 High-Fidelity 2x Master Mix (New England Biolabs, M0494X) was used to amplify the sgID-BC region from 32 µg of genomic DNA using unique dual-indexed primers.
GEO Accession
GSM4377000