genomic DNA (gDNA) was extracted and fragmented with Bioruptor (Connecticut, USA) into fragment sizes of 200–500 bp Sonicated gDNA was used for end-repair and adaptor ligation. The adaptor-ligated gDNA was denatured and incubated with an antibody (Epigentek, A-1014) conjugated on Protein A+G Magnetic beads (Millipore, 16-663). Immunoprecipitated DNA was amplified by PCR and subjected to Illumina sequencing.
GEO Accession
GSM3964208