Initial extraction and polyA enrichment were performed in the NCATS laboratories. Total RNA samples were prepared from cultured cells using Qiaquick kit (Qiagen, Germantown, MD) and selected for polyA+ transcripts using the Qiagen Oligotex midi kit. The resulting samples were then depleted of ribosomal sequences using two rounds of RiboZero Gold™, Illumina Inc. TruSeq Protocol by the NHLBI-NIH sequencing core (NHLBI-NIH DNA Sequencing and Genomics Core, Bethesda, MD, https://www.nhlbi.nih.gov/science/dna-sequencing-and-genomics-core). RNA libraries were prepared for sequencing using standard Illumina protocols (TruSeq) RNA-Seq library production (TruSeq) for Hiseq 3000
GEO Accession
GSM4520610