Tumour cells were collected in FACS buffer, centrifuged for 8 minutes at 500g, and were lysed in RLT lysis buffer. Total RNA was extracted with the RNeasy Midi kit (Qiagen). The concentration and integrity of RNA samples was determined with the Bioanalyzer RNA 6000 Pico Kit (Agilent).
GEO Accession
GSM3265334