We used the Invitrogen PureLink Genomic DNA Mini kit following the manufacturer's instructions for isolation of gDNA from gram-negative bacteria. To examine adenine and cytosine methylation across the 5 Mb E. coli genome strain K12 substrain MG1655, SMRTbell library using C2 chemistry for use in a 120-minute movie protocol was conducted
GEO Accession
GSM3264688