We generated RNH1 knock out K562 cells using the CRISPR/Cas9 method. Total RNA was isolated from wild type and RNH1 deficient K562 cells. Illumina TruSeq Stranded mRNA protocol Illumina TruSeq Stranded mRNA protocol. We prepared Illumina TruSeq stranded mRNA libraries. The libraries were subjected to a preparative size selection on a PippinHT instrument to exclude library molecules with insert sizes of less than 300 bp.
Experimental Factor: wild-type
genotype