Cells were rinsed in PBS and harvested using the Trizol reagent. RNA was then separated via the introduction of chloroform, and purified on Qiagen RNeasy Mini Kit columns (Qiagen #74106). RNA-seq libraries were created using the SMARTer Stranded Total RNA Sample Prep Kit (Takara Bio #634875) from 500ng initial RNA. Samples were sequenced on the NextSeq 500 (Illumina) to a minimum depth of 25 million reads per sample.
GEO Accession
GSM4697641