1. step:initial denaturation:98degC_30s;25times;denaturation:98degC_10s;annealing:55degC_30s;extension:72degC_60s;final extension:72degC_10min;storage:10degC_infinity; 2. step:initial denaturation:98degC_30s;10times;denaturation:98degC_9s;annealing:55degC_30s;extension:72degC_60s;final extension:72degC_10min;storage:10degC_infinity