Skin samples were collected directly into RNA Later reagent (Ambion) and frozen at -80°C until further use. Tissue disruption and homogenisation was carried out in RTL buffer (Qiagen) using TissueRuptor (Qiagen) on ice. RNA was extracted with RNeasy Fibrous Tissue Mini Kit (Qiagen) according to the manufacturer's instructions. Next Generation Sequencing was carried out with Illumina reagents (TruSeq Targeted RNA Index Kit and TruSeq Targeted RNA Custom Panel Kit) at the Deep Seq Facility (The University of Nottingham). Targeted RNAseq
GEO Accession
GSM4147160