To generate single-cell suspensions, femoral arteries isolated from both male and female mice (8 weeks old) were further digested in collagenase I for 30-60 min as described. In detail, femoral arteries were cut into 1mm small pieces, and these small pieces were digested by 1.5 mg/ml collagenase (Gibco, 17018029) at 37°C, during this process, artery pieces was frequent stirred to ensure full digestion. After sufficient digestion, cells were filtered through a 40-μm cell strainer, and the collected cells were centrifuged 5 min at 600g speed. Subsequently, cell pellet was suspended in PBS or DEME for further experiments Single cell library was generated using Chromium™ Single Cell 3' Library Kit v2
GEO Accession
GSM4147025