Total RNA was extracted using standard hot-phenol procedure Ribosomal RNAs were depleted from total RNA using the RiboMinus Eukaryote v2 Kit (Life Technologies). Strand-specific total RNA-Seq libraries were prepared from 125 ng of rRNA-depleted RNA using the TruSeq Stranded Total RNA Sample Preparation Kit (Illumina). Paired-end sequencing (2 x 50 nt) of the libraries was performed on a HiSeq 2500 sequencer.
GEO Accession
GSM2300129