Total RNA was isolated using the Rneasy kit, with on-column DNase treatment. cDNA was generated and amplified using the NuGen Ovation RNAseq kit. Libraries were generated with Illumina adapters, and barcoded to allow all 6 samples for a single experiment (3 biological replicates for each of two cell populations) to be run on a single lane.
GEO Accession
GSM1447423