Total RNA containing the microRNA fraction was isolated using miRNeasy kit (Qiagen) according to the manufacturer's protocol. MiRNA sequencing libraries were prepared using QIAseq miRNA library kit (Qiagen) following the manufacturer's instructions and followed by sequencing on an Illumina NextSeq instrument. Using Qiagen's Geneglobe Software, sequencing reads were aligned to the human reference genome assembly (hg19) followed by transcript assembly and estimation of the relative abundances
GEO Accession
GSM3263610