Total RNA was extracted from shoot tips using the Qiagen RNeasy Midi RNA isolation kit according to manufacture protocol and as described by Mathiason et al 54, DNAased and stored at -80°C. DNA was removed by incubation with 1 unit per microgram (μg) RNase-free DNase (Promega, Madison WI) at 37°C for 30 min. RNA was purified using RNeasy plant mini columns (Qiagen, Valencia CA). RNA quality and quantity were verified with an Agilent (Santa Clara, CA) 243 Bioanalyzer RNA 6000 nano chip. RNA seq libraries for LD and SD shoot transcriptome at 7 and 21 days were prepared and sequenced by Illumina HiScanSQ (43bp, single strand) at the Cornell University, Institute of Biotechnology Genome Facility (Ithaca, NY, USA).
GEO Accession
GSM2522400