C57BL/6 wild type and multiple system atrophy transgenic mice (Tg(Plp1-SNCA)1Haa strain backcrossed in C57BL/6 in over 20 generations) at an age of 2-3 months were used in this study.  The transgenic mice have a transgenic insertion of the human wild-type alpha synuclein driven by the  proteolipid protein promoter. Transgenic protein is detected in oligodendrocytes but in no other brain cell type. The mice were sacrificed by cervical dislocation, brains were quickly extracted and the substantia nigra and striatum were dissected on ice. Three pools of substantia nigra and striatum, respectively, from 5 mice each were prepared and frozen in liquid nitrogen.  Total RNA was isolated using Tri-Reagent (Sigma-Aldrich, St. Louis, Missouri, USA) according to the   manufacturer's instructions and dissolved in DEPC-water. RNA was then submitted to polyA-enrichment  (Dynabeads mRNA Purification Kit, Life technologies) using 6 g of total RNA from each pooled sample. With   the polyA-enriched RNA as input, libraries were generated with the Ion Total RNA-Seq Kit v2 (Ion torrent, Life   Technologies) and barcoded following the manufacturers instructions, except that the final bead-based size selection was adjusted to favor longer fragments (average read lengths >150 bp).
Experimental Factor: wild type
genotype