Spleen samples of uninfected chickens and chickens suspected of having ALV-J infection were collected independently, washed in RNase-free water, transferred to tissue-cryopreservation tubes, immediately submerged in liquid nitrogen. Total RNA was harvested using Illumina TruSeq Stranded Total RNA with Ribo-Zero Gold kit. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM3346396