iFA model: In-vitro cultures of PF and cells in the iFA model were washed once with PBS and total RNA from the samples were extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. Libraries for RNA-Seq were prepared with the Nugen human FFPE Kit (LSC). The workflow consisted of cDNA generation, end repair to generate blunt ends, adaptor ligation, adaptor cleavage and PCR amplification. Different adaptors were used for multiplexing samples sequenced in the same lane. The libraries of aggregate iSDC cultures treated with AA5 or DMSO were sequenced with 75-bp single-end reads on the Illumina Nextseq500 system.  The libraries of aggregate iSDC cultures at day 4 and day 13 were run with 50-bp paired-end reads on the Illumina system (model HiSeq 2000).
GEO Accession
GSM2611449