Okazaki fragments were enriched by sequential elutions from ion exchange columns as previously described (Smith, D. J., and Whitehouse, I. (2012). Intrinsic coupling of lagging-strand synthesis to chromatin assembly. Nature 483, 434-438). Libraries were constructed as previously described (Smith, D. J., and Whitehouse, I. (2012). Intrinsic coupling of lagging-strand synthesis to chromatin assembly. Nature 483, 434-438) with the following changes: after adaptor ligation, samples were cleaned using a PCR purification kit (GeneJET, ThermoFisher) instead of S-300 columns, and second-strand synthesis was carried out by Taq polymerase (NEB) at 50C. Libraries were purified using magnetic beads (HighPrep PCR Clean-up, MagBio) before and after amplification.
GEO Accession
GSM4147889