PAEC pellets were treated with hypotonic buffer for 10 min on ice and then were homogenized using a glass homogenizer.  Nuclear pellets were re-suspended in RIPA buffer (Millipore) and incubated for 30 min on ice. Chromatin was sheared with SFX250 Sonifier (Branson, Dunbury, CT). The ChIP-DNA samples were end-repaired and A-tailed.  Illumina TruSeq adapters (Illumina, San Diego, CA) were ligated and size-selected from the gel before performing PCR amplification.
GEO Accession
GSM4147856