Lungs were purfused, removed, and dissociated to make single suspension. After staining with proper antibody of surface markers, cells were sorted usign BD Aria II sorter. The RNA was harvested from the sorted cells using Arcturus PicoPure RNA isolation kit. Illumina TruSeq RNA Sample Prep Kit v2 (Cat#RS-122-2001) was used with 16-33ng of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols(Illumina TruSeq RNA Sample Prep Kit v2 (Cat#RS-122-2001)).
GEO Accession
GSM1447109