cells were harvested, washed in 1X PBS and then RNA was harvested using Trizol reagent. RNA was DNase treated (NEB) and then cleaned with Qiagen MinElute Cleanup kit. 200 nanograms of RNA per sample were used to prepared dual-indexed strand-specific cDNA library using KAPA mRNA Hyperprep Kit (Roche)
GEO Accession
GSM4696741