Mice were bred and allowed to age until a humane endpoint was reached. Primary tumors and lung metastases were isolated and snap frozen in cryo vials using liquid nitrogen. Tissue was stored at -80 and gDNA was later isolated using the gDNA purification using ZR-Duet DNA/RNA MiniPrep kit from Zymo Research.  (cat.no. D7001) Whole genome sequencing library preparation was performed using the TruSeq DNA Sample Prep kit FC-121-1001.  Samples were barcoded, pooled and sequenced on an Illumina HiSeq4000 to a depth of ~10x per sample. For RNA sequencing, the preparation of mRNA libraries and mRNA sequencing was performed by the Sequencing Facility using the HiSeq2500 instrument with Illumina TruSeq v4 chemistry.
GEO Accession
GSM4296440