80-95 wing imaginal discs were dissected at room temperature in PBS and quickly transferred immediately to ice before snap freezing in liquid nitrogen. Discs were ground in RLT buffer and purified with RNeasy (Qiagen). RNA was then DNased and re-purified with RNeasy. 2 µg of total RNA was depleted of ribosomal RNA with the Ribo Zero kit (Epicentre #MRZG12324) and libraries were made with the TruSeq RNA sample Prep Kit (Illumina #RS-122-2001).
GEO Accession
GSM1449084