Total RNA was isolated from MCF10A cells using Trizol (Invitrogen). Ribosomal RNA was removed using the Ribominus kit (Invitrogen). cDNA library was generated and sequenced using an Illumina Hiseq 2500 (1 × 100bp runs), as instructed by Illumina’s RNA-seq protocols.
GEO Accession
GSM1829628