S.cerevisiae strains used were BY4716 and RM11-1A. Cells were grown to exponential phase in synthetic medium with 2% glucose (SDall) at 30°C ChIP was performed using anti-H3K14ac antibody as described in Nagarajan et al. PLoS Genetics 2010 6(4):e1000913. Libraries were built following the procedure of the BioScientific NEXTflex™ ChIP-Seq Kit and NEXTflex™ ChIP-seq Barcodes.
Experimental Factor: strain
RM11-1a
Experimental Factor: protocol type
ChIP-Seq