PBMCs were isolated from these samples via a standard Ficoll-hypaque density gradient. After PBMCs were obtained, RNA was isolated utilizing the guanidinium thiocyanate phenolchloroform  extraction method. Transcriptomic libraries were prepared with the Illumina TruSeq Stranded mRNA Sample Preparation kit with a minimum input of 0.1ug of total RNA as directed by the manufacturer in accordance with the low sample protocol.
GEO Accession
GSM4709425