Plantlets were collected, frozen in liquid nitrogen and grind. RNA was extracted using Qiagen RNeasy® Plant kit according to the supplier's instructions (QIAGEN S.A, France) including the DNAse treatment. RNA libraries were prepared for sequencing using standard Illumina protocols. TruSeq Stranded mRNA SamplePrep Guide 15031047_D protocol (Illumina®, California, U.S.A.). The RNA-seq samples have been sequenced in paired-end (PE) with a sizing of 260 bp and a read length of 100.
GEO Accession
GSM3264305