Blood: 200μL of PB was placed in 600μL of RNAlater then later RNA was extracted with the QIAamp RNA Blood Mini Kit (QIAGEN, Hilden, Germany), CTC were sorted into Prelude Direct Lysis Module (NuGEN, San Carlos, CA). Tumors: RNeasy FFPE kit (QIAGEN) Blood: NuGEN Ovation RNA System V2, CTC: the Ovation Single Cell RNA-Seq System (NuGEN); Tumors: NuGEN Ultra Low Library System V2. RNA-Seq with poly-A isolation
GEO Accession
GSM3040947