Total RNAs from cultured cells (0.5 x 10^6 - 1 x 10^7) were extracted with Trizol (Invitrogen) according to the manufacturer's instruction, followed by DNase I treatment at 37 °C for 30 mins (Ambion, DNA-freeTM Kit). The polyadenylated RNA population was purified by oligo(dT) magnetic beads (Invitrogen) from 5 μg of total RNAs. Library was prepared by using Illumina TruSeq Stranded Total RNA HT Sample Prep Kit (P/N 15031048) and Illumina TruSeq Small Sample Prep Kit (P/N 15004197).
GEO Accession
GSM1464571