3D culture hydrogel was dissociated and human neuronal cells were sorted from dissociated hydrogel-cell suspension into a buffer preserving RNA integrity, which was then centrifuged and then stored at -80 °C. Total RNA isolation was performed using a kit following the manufacturer's protocol. RNA-Seq libraries were prepared following the Smart-seq2 protocol. 1 ng of total RNA was used. cDNA was synthesized and then used in each NexteraXT (Illumina) reaction.
GEO Accession
GSM3040489