Blood samples were obtained from healthy adults via venipuncture into vacutainer tubes. A buffy coat was separated from whole blood using a Ficoll-Paque (GE Healthcare) centrifugation gradient, and remaining erythrocytes were lysed using a red cell lysis buffer (Qiagen). RNA was extracted using the miRVana miRNA Isolation kit (Life Technologies). Total RNA-seq libraries were prepared with 1ug purified total RNA input using the TruSeq Stranded Total RNA Sample Prep Kit with Ribo‐Zero Globin (Illumina). Samples were muliplexed, libraries pooled, and run on two flowcells with the Illumina HiSeq 2000 sequencing system. Barcode IDs are as follows: RBC_1-CGATGT, RBC_2-TGACCA, RBC_3-ACAGTG, PBMC_1-GCCAAT, PBMC_2-CAGATC, PBMC_3-CTTGTA, D8_1-AGTCAA, D8_3-GTCCGC.
GEO Accession
GSM1830787