The dissection and microglia isolation steps were carried out on ice or at +4°C. Cortex and hippocampus were dissected and finely chopped, and transferred to a tuve containing ice-cold Hibernate A medium without phenol red. Single cell suspension prepared using the Adult Brain Dissociation kit. Single live microglia cells (CD11b+/DAPI-) were sorted using BD FACSAria III Single cell retrotranscription and preparation of sequencing libraries was performed using a modified SmartSeq2 protocol (Picelli et al., 2013, 2014; Trombetta et al., 2014). We used 5'-biotinylated primers throughout the protocol
GEO Accession
GSM3644764