DRGOs were treated with Trypsine-EDTA 0.25% (Gibco) and DNaseI 200ug/ml (Sigma) for 30 minutes at 37°C. Then they were gentle triturated pipetting up and down, washed with PBS and counted with Trypan Blue (Invitrogen). Libraries were sequenced with a depth of 50K reads/cell. Single cell RNA sequencing was performed through the Chromium platform (10X Genomics). Cells were separated into droplet emulsion using the Chromium Single Cell 3' Solution (V3.0) and Single-cell RNA-seq libraries were prepared according to the Single Cell 3' Reagent Kits User Guide (V3.0).
GEO Accession
GSM4456095