Lysates were clarified from sonicatd nuclei and GFP- or histone-DNA complexes were isolated with antibody ChIP-seq libraries were constructed using KAPA Hyper Prep Kit (KAPA Biosystems) according to the manufacturer's instructions. ChIP-seq libraries were quantified by Tapestation (Agilent) and sequenced on an Illumina NEXTseq (Illumina, San Diego, CA, USA) with 75-base single-end mode.
GEO Accession
GSM4585003