We isolated mouse glomeruli with Dana-beads/magnetic concentration and dissociated them into single cells using enzymes. The cell suspension was then loaded onto a Fluidigm C1 Single-Cell Auto Prep System, which separated the single cells and lysed them for RNA extraction in individual wells. Then, we performed qPCR analyses of glomerular cell markers to determine the identity of each sample. Fifteen mesangial cells and 15 endothelial cells' cDNA samples were mixed and then divided into 3 aliquots, followed by sequencing library construction. cDNA libraries were made automatically in the Fluidigm C1 platform.
GEO Accession
GSM2363334