Lysates were clarified from sonicated fixed nuclei (for embryos) and from sonicated fixed hand-dissected CNS and imaginal disc tissues (for 3rd instar larvae). Histone-DNA complexes were isolated with antibody. D. pseudoobscura chromatin was spiked in at a 1:1 ratio of dm / dp chromatin before the addition of the antibody. Libraries were prepared according to Illumina's instructions accompanying the TruSeq kit.
GEO Accession
GSM4458800