Total RNAs from breast cancer cells were extracted with TRIzol The amount of 2 μl of 14 μl eluted RNA was reverse transcribed with High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific, USA). Once successfully immunoprecipitated methylated RNA was confirmed, further transcriptome-wide interrogation was pursued by deep sequencing using SMARTer Stranded Total RNA-Seq Kit version 2 (Pico Input Mammalian; 634413; Takara/Clontech, Japan) according to the manufacturer's protocol. A purified library was quantified using a Qubit Fluorometer (Thermo Fisher Scientific, USA), and the size distribution was checked using the Bioanalyzer 2100 system (Agilent Technologies, USA). The library preparations were sequenced on an Illumina Novaseq 6000 platform and 150 bp paired-end reads were generated.
GEO Accession
GSM4709652