Total RNA was extracted using the TRIzol reagent (Life Technologies) and purified from residual DNA contaminant using the TURBO DNA-freeTM Kit (Ambion, Austin, TX) according to the manufacturer’s instructions. RNA quality was checked on the Agilent 2100 Bioanalyzer and quantity was measured using the Qubit instrument (Life Technologies). Library construction were performed according to Illumina's instructions.
GEO Accession
GSM2523085