Neural folds were dissected from HH9 embryos (n=120 per replicate) in Ringers solution and dissociated in Accumax (Innovative Cell Technologies, #AM105). Whole HH9 embryos were prepared in parallel as control samples (n=20 per replicate). After dissociation cells were crosslinked, washed in cold PBS and submited to the HiChIP protocol as published by (Mumbach, et al. 2016). In situ contact libraries were generated according to the HiChIP protocol published by (Mumbach, et al. 2016). DNA digestion was performed with MboI and H3K27ac antibody was used for fragment pulldown. DNA fragments were tagmented and PCR amplified. After size selection, libraries were quantified and sequenced with paired-end 75bp reads on an Illumina NextSeq500 instrument.
GEO Accession
GSM4520630