Cell pellets were snap frozen and shipped on dry ice to Active Motif where subsequent steps were performed according to previously published pipeline (de Almeida Nagata DE et al. Cell Rep 2019;27(1):269-81) Illumina sequencing libraries were prepared from the ChIP and Input DNAs by the standard consecutive enzymatic steps of end-polishing, dA-addition, and adaptor ligation. Chip-Seq: After a final PCR amplification step, the resulting DNA libraries were quantified and sequenced on Illumina's NextSeq 500 (75 nt reads, single end).
GEO Accession
GSM4456465