Luminal samples from the ileum and cecum of 3-day old infant rabbits infected with V. cholerae were snap-frozen in liquid nitrogen for subsequent phenol:chloroform RNA isolation. Contaminating DNA was removed using the DNA-free kit (Applied Biosystems) and RNA was stored at -80°C. The Ovation Complete Prokaryotic RNA-Seq Library Systems (NuGEN) was used for cDNA synthesis and library preparation of 200 bp (mean size) fragments. cDNA library quantification was performed using the Agilent 2200 TapeStation.
GEO Accession
GSM3884920