Total RNA was extracted using TRIzol Reagent (Invitrogen, Carlsbad, CA) and purified by column NucleoSpin® RNA clean-up kit（Macherey-Nagel,Germany）RNA quality and concentration was determined by analysis with an Agilent 2100 bioanalyzer. To isolate bacteroids RNA, total nodules RNA passed though MICROBEnrich™ kit (Ambion) according to the manufacturer's instruction. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM1168509