Total RNA was extracted using Trizol and 1-bromo-3-chloropropane and precipitated with isopropanol. RNA extracts were then treated with DNaseI to remove potential DNA contamination. mRNA was enriched using NEBNext Ply(A) mRNA Magnetic Isolation Module, mRNA was fragmented at 94 °C for 15 minutes and used as input for library preparation. Library was prepared using NEBNext® Ultra™ II RNA Library Prep Kit for Illumina as per manufacturer's protocol.
GEO Accession
GSM4709430