ATACseq was performed as in (Buenrostro et al, Nature methods 10, 1213-1218 (2013)) with the following modifications. 100.000 cells were washed once with 100µl PBS and resuspended in 50µl lysis buffer (10mM Tris-HCl pH 7.4, 10mM NaCl, 3mM MgCl2, 0.2% IGEPAL CA-630). The nuclei suspension was then centrifuged for 10min at 500g at 4C. The 50µl transposition reaction (25µl TD buffer, 2.5µl Tn5 Transposase and 22.5µl Nuclease Free H2O) was added to the isolated nuclei and incubated at 37C for 45min. DNA was isolated using Qiagen MinElute Kit following the manufacturer instructions. Library amplification was done by two sequential PCR reactions (8 and 5 cycles, respectively). After the first PCR reaction the library was size selected (for fragments below 700bp) with AmpureXP beads followed by a second PCR reaction. Libraries were purified with Qiaquick PCR purification and integrity checked on a Bionalyzer before sequencing.
GEO Accession
GSM1830115