RNA was extracted by using the RNeasy Mini Kit, followed by treatment with RNase-free, DNase to remove contaminating genomic DNA. RNA (200 ng) RNA was reverse transcribed by using the Illumina TruSeq Stranded Total mRNA kit. Libraries were sized on an Agilent Bioanalyzer, and their concentrations were validated by qPCR.
GEO Accession
GSM4697665