RNA was extracted with Trizol (Invitrogen) and further purified on RNeasy columns (Qiagen) with on column DNase digestion. Ribosomal RNA was depleted using the RiboMinus kit (Invitrogen).  1 µg rRNA-depleted RNA was used for the construction of sequencing libraries Illumina RNA-seq libraries were prepared from rRNA-depleted samples using the standard Illumina protocol. SOLiD RNA-seq libraries were prepared from a pool of all of 15 ICM samples and a pool of all of 15 Ctrl samples using the standard SOLiD strand-specific library protocol.
GEO Accession
GSM1171563