RNA was extracted using an RNeasy (Qiagen, UK) according to manufacturer's instractions.  Libraries were perepared according to standard Illumina protocols.  Four independent biological libraries were prepared for each sample to facilitate the expression detection and variance estimation. Multiplexing was achieved using in-house designed barcoding adapters Four biological replicates of either siHOXC11 or scrHOXC11 sample were sequenced on a flow cell. Two replicates, one from each sample, were multiplexed and loaded on a single lane.
GEO Accession
GSM1828395