Mobilized Peripheral Blood CD34+ Cells (AllCells) were grown in StemLine II media containing Glutamine (Sigma) with 20% BIT (StemCell Technologies) and 1% Penicillin/Streptomycin (Gibco). Cells were supplemented with growth factors IL-3 at 20ng/mL (Invitrogen), SCF at 50ng/mL (Invitrogen), and Erythropoietin (Epo) at 3U/mL (Calbiochem). Cells were isolated on Day 8 of in vitro erythroid differentiation. The cells were rinsed twice in cold PBS, then RNA was extracted using the miRVana miRNA Isolation kit (Life Technologies). Total RNA-seq libraries were prepared with 1ug purified total RNA input using the TruSeq Stranded Total RNA Sample Prep Kit with Ribo‐Zero Globin (Illumina). Samples were muliplexed, libraries pooled, and run on two flowcells with the Illumina HiSeq 2000 sequencing system. Barcode IDs are as follows: RBC_1-CGATGT, RBC_2-TGACCA, RBC_3-ACAGTG, PBMC_1-GCCAAT, PBMC_2-CAGATC, PBMC_3-CTTGTA, D8_1-AGTCAA, D8_3-GTCCGC.
GEO Accession
GSM1830788