Cells were harvested with cold PSB and pellets were kept frozen at -80 deg. Total RNA was extracted using the Norgen Biotek total RNA purification plus kit (Cat #48300). RNA purity was assessed using Qubit RNA assay and BioAnalyzer RNA Nanochip tracings. RNA libraries were prepared for sequencing using the New England Biolabs NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with the protocol for use with NEBNext Poly(A) mRNA magnetic isolation module.  We used 200ng of total RNA for the construction of sequencing libraries.
GEO Accession
GSM2957679