Total RNA was extracted from a 20mg piece of the large lobe of six livers (3 per diet group) using the Qiagen miRNeasy Mini kit (cat# 217004, Qiagen) per the manufacturer's instructions. Libraries were prepared from the extracted RNA using two different kits, the QuantSeq 3'mRNA-Seq Library Prep Kit-FWD (cat #15, Lexogen, Vienna, Austria), and the KAPA Stranded mRNA-Seq Kit (cat #KK8421, Kapa Biosystems, Wilmington, MA),  per the manufacturers' instructions using 1 μg of RNA per library.
GEO Accession
GSM3265107