total liver cells were isolated by collagenase digestion, follwed by FACS purification for living UV autoflorescent hepatic stellate cells, which were either used as controls or subjected to in vitro differentiation activated hepatic stellate cells were also isolated by collagenase digestion and FACS purified as living CD45- and CD31- cells in both cases 50.000 cells were then resuspended in cold PBS + 0.1 % BSA and subjected to 10x single cell library generation according to manufacturers protocol cDNA