S.cerevisiae strains used were BY4716 and RM11-1A. Cells were grown to exponential phase in synthetic medium with 2% glucose (SDall) at 30°C ChIP was performed at single-nucleosome resolution using formaldehyde fixation followed by micrococcal nuclease digestion and isolation of 150 bp fragments. Three BY and RM independent cultures were processed for nucleosome mapping  (MNase-seq samples). Libraries were built following the procedure of the BioScientific NEXTflex™ ChIP-Seq Kit and NEXTflex™ ChIP-seq Barcodes.
Experimental Factor: strain
RM11-1a
Experimental Factor: protocol type
MNase-Seq