Total RNA will be isolated from liver tissues using TRIzol® reagent (Invitrogen). Total RNA (15 μg) for each sample will be used for purifying the poly(A)-containing mRNA molecules, RNA ampliﬁcation, and synthesis of double stranded cDNAs that will be ligated to adapters, following the Illumina (San Diego, California, USA) TruSeq RNA Sample Prep guidelines. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM2300548