50 mL of Chromera cultures were pelleted by spinning the cultures at 3,000 x g for 5 min. Pellets were suspended in 1 ml 0.2 μm sterile FSW and centrifuged at 3,000 x g for 5 min. Pellets were snap frozen in liquid nitrogen and stored at -80°C until further treatment. Total RNA was isolated from ~80 mg of the frozen Chromera pellets using the RNAqueous® Total RNA Isolation Kit (Ambion). RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM4146917