Cells were lysed in mammalian polysome buffer, digested with RNase I, loaded on a MicroSpin S-400 HR colum (GE Healthcare) and RNA was extracted using Trizol LS as described in Calviello et al. 2016 (doi:10.1038/nmeth.3688) libraries were constructed as described in Calviello et al. 2016 (doi:10.1038/nmeth.3688)
GEO Accession
GSM3966952