Genomic DNA was extracted using DNeasy Blood &                     Tissue kit (Qiagen, 69504) for each iPS cell lines. Then DNA was processed using                     the SureSelectXT Mouse Methyl-Seq Reagent kit (Agilent, 931052). After capture,                     the bisulfite conversion of the eluted DNA was performed by EZ DNA                     Methylation-Gold kit (Zymo Research, D5005). Then the converted DNA was                     amplified using the PCR reagents from SureSelectXT Mouse Methyl-Seq Reagent kit                     (Agilent, 931052) according to the manufacturer's                     recommendations.