Following sorting, cells were spun and resuspended in 100 uL RLT buffer from the RNAeasy micro kit (Qiagen). RNA in RLT and RNA extracted via RNAeasy kit. Libraries were constructed and sequenced by Amaryllis (Emryville, CA) via single-end sequencing to generate 30M reads per sample. Single-end sequencing was used to generate 30M reads per sample.