RNA was extracted using chloroform extraction from Trizol, after which RNA was further purified using the DirectZol RNA miniprep kit (Zymo Research) mRNA was isolated from 100 ng total RNA by polyA selection, fragmented and reverse-transcribed into cDNA. Adapters were ligated to individual samples to allow multiplexing samples (8-9/lane).
GEO Accession
GSM2365477