Total RNA was isolated from cells by RNAiso Plus (Takara, Dalian, China). Libraries were prepared from 100-1000ng total RNA using the VAHTSTM mRNA-seq v2 Library Prep Kit for Illumina® (Vazyme, Nanjing, China) according to the manufacturer’s protocol. Libraries were validated by the 2100 BioAnalyzer (Agilent, Beijing, China), normalized, and pooled for sequencing.
GEO Accession
GSM2610270