Lysates were clarified from sonicated nuclei andNFAT1-v5-DNA complexes were isolated with antibody. Illumina TruSeq DNA version 2 kit according to the manufacturer's protocol, with the following modifications; 30X adaptor dilution, 18 PCR cycles of amplification. Batches of 5-8 samples were sequenced as a multiplex in individual lanes of an Illumina HiSeq 2000 apparatus using a single-end run (1X51) together with the SBS kit v3. Reads were mapped to the mouse genome (version mm9) with bowtie 1 (Langmead et al., 2009) using default parameters.
GEO Accession
GSM1463320