RNA was extracted from jejunum enterocytes isolated from 18-week-old mice using Trizol (Sigma-Aldrich) followed by column purification using the RNeasy column purification kit (Qiagen), with on-column DNase (Qiagen) digestion for all samples. 1ug of total RNA was used for library preparation. Illumina TruSeq stranded mRNA.
GEO Accession
GSM4456005