Epididymal ducts were dissected from E18.5 control and TCre-cKO embryos. Total RNA was extracted using Qiagen RNeasy Plus Mini Kit (Valencia, CA) according to manufacturer's instructions. Quality control of RNA was conducted using TapeStation and qPCR methods at the Genewiz (South Plainfield, NJ). cDNA synthesis and library construction were performed using Illumina Truseq RNASeq (Illumina Inc. San Diego, CA). Four control and four knockout sample replicates were analyzed on an Illumina HiSeq 2000 instrument at Coulter Genewiz (South Plainfield, NJ).
GEO Accession
GSM2610556