Total RNA was isolated using Tri-Reagent (Sigma) and subsequently digested with DNase I using TurboDNA (ambion), following manufacturer’s specifications. mRNA was enriched with oligoT, fragmented to an average size of 200 nt and retrotranscribed using random hexamers. cDNA was then amplified and sequenced. DNaseI-digested total RNA was processed and sequenced by BGI-Hong Kong. RNAseq samples were  sequenced using the Illumina HiSeq 2000 instrument.
GEO Accession
GSM1167587