Total RNA was extracted using the RNeasy extraction kit (Qiagen, cat# 75142) following manufactures instructions that included an in-column DNAse digestion.  Poly A RNA sselection.  Quality of the RNA was then confirmed using Agilent 2100 Bioanalyzer. RNA library preparation and high-throughput sequencing were performed on an Illumina HiSeq 2000 sequencing system following the protocol recommended by Illumina for sequencing total RNA samples.   Strand-specific library prep.  Sequencing was done for each biological replicates at 1 x 75bp strand specific by the Yale Center for Genome Analysis
GEO Accession
GSM1164131