DNA of each collected tissue was extracted using DNeasy Blood & Tissue Kit (Qiagen). Genomic DNA was digested by restriction enzyme MspI. Subsequently, Ilumina paired-end protocol, consisted with end repair, A-tailing and methylated-adaptor ligation, was performed to construct libraries. 40-220bp fragment was selected and purified, then treatment with bisulfite by ZYMO EZ DNA Methylation-Gold kit. All bisulfite converted products were amplified by PCR for library preparation and only qualified library was sequenced with IlluminaGAII.
GEO Accession
GSM2522182