48 h after transfection, total RNAs were extracted with RNAiso Plus (TaKaRa) reagent. cDNA was synthesized by PrimeScript TM RT reagent Kit with gDNA Eraser (TaKaRa) following standard protocol. KOD DNA polymerase was used to amplify the CDR1-3 encoding sequences . Library construction and high-throughput sequencing were conducted by Novogene (Tianjin, China). Sequencing libraries were generated using TruSeq® DNA PCR-Free Sample Preparation Kit (Illumina) following the manufacturer's recommendations, and the index codes were added. The library quality was assessed on the Qubit Fluorometer and Agilent Bioanalyzer 2100 system. At last, the library was sequenced on an Illumina NovaSeq 6000 system and 250-bp paired-end (PE) reads were generated.
GEO Accession
GSM4696410