Epithelium from proximal 1/3 of mouse intestine was isolated by incubation with 5mM EDTA in PBS for 45 minutes at 4°C. Cells were snap-frozen in liquid nitrogen and used for subsequent ChIP analysis. Whole cell lysates were sonicated and incubated with 1:1.5 combination of Foxa1 (Abcam 23738) and Foxa1 (Abcam 5089) overnight for immunoprecipitation. ThruPLEX DNA-seq Kit (Rubicon Genomics) was used to prepare libraries, and 75 bp single-end reads were sequenced on an Illumina NextSeq 500 instrument.
GEO Accession
GSM3040280