Genomic DNA was isolated using Qiagen DNA mini kit. 3 μg of genomic DNA were sonicated in a Covaris S sonicator. DNA fragments which were about 150  bp in size were then subjected to enrichment with 60 μg MBD2-Fc protein in the presence of a salt gradient. The DNA fractions highly enriched for methylated DNA (eluted with 1 M NaCl) were subjected to next-generation sequencing. NEBNext ChIP-seq Library Prep Reagent Set for Illumina
GEO Accession
GSM1464820