RNA was extracted using Trizol reagent followed manufacture protocol (Invitrogen). 2ng of total RNA underwent cDNA pre-Amplification using CloneTech SMART-Seq v4 Ultra Low Input RNA Kit followed manufacture instruction with 12 cycles of PCR. Library preparation was followed by using KAPA Hyper prep kit according to instruction provided by KAPA Biosystems, cDNA input was normalized to 10 ng per sample as library input, with 8 cycles of PCR.
GEO Accession
GSM4146714