12 rats were collected from rats population and split randomly into control and exercise two equal sized groups randomly.  Sample Rats in treatment group underwent a 13-week downhill treadmill running exercise programme. RNA was extracted using Trizol and RNA was  purified using Qiagen RNAEasy kit. RNA samples were ribosomally depleted using Illumina's Ribo-Zero kit (human, mouse, rat. Normal input). They were then analysed using Qubit for quantity and on Bioanalyser for quality. The samples were then put through the Illumina Script Seq 2 protocol to fragment, tag and amplify the samples. They were then analysed using Qubit for quantity and on Bioanalyser for quality. Amplified libraries were multiplexed and the quality and quantity of the pool were assessed using Qubit and the Agilent 2100 Bioanalyser and subsequently by qPCR using the Illumina Library Quantification Kit from Kapa on a Roche Light Cycler LC480II according to manufacturer's instructions.
Experimental Factor: stimulus
control