Separation of gDNA and mRNA was performed as described previously. Briefly, samples were incubated at 72 °C for 3 min, the Biotin-oligo-dT primer was hybridized to poly (A)+ tail mRNA. Dynabeads in 4 μL (65001, Life technologies) were added to capture poly (A)+ tail mRNA, collected by a magnet, and the gDNA supernatant transferred to a fresh PCR tube. Single-cell cDNA was synthesized in the tubes containing mRNA according to Smart-seq2 protocol. The libraries were prepared by using TruePrep DNA Library Prep Kit V2 for Illumina® (TD503-02, Vazyme Biotech) according to the instruction manual.
GEO Accession
GSM2607608