See http://www.nature.com/nprot/journal/v7/n12/full/nprot.2012.137.html
EXPERIMENT_TYPE
DNA Methylation
EXTRACTION_PROTOCOL
See http://www.nature.com/nprot/journal/v7/n12/full/nprot.2012.137.html
EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR
Covaris
EXTRACTION_PROTOCOL_SONICATION_CYCLES
Sonicate the genomic DNA in 120 µl of EB buffer to the desired size range. We typically sonicate for 55 s using a Covaris system, with a 10% duty cycle, intensity set at 4 and cycle per burst set at 200 in order to achieve the size range of 200-500bp. 
DNA_PREPARATION_INITIAL_DNA_QNTY
5ug
DNA_PREPARATION_FRAGMENT_SIZE_RANGE
200-500bp
DNA_PREPARATION_ADAPTOR_SEQUENCE
Standard Illumina adaptor sequence
DNA_PREPARATION_ADAPTOR_LIGATION_PROTOCOL
TruSeq
DNA_PREPARATION_POST-LIGATION_FRAGMENT_SIZE_SELECTION
250-600
BISULFITE_CONVERSION_PROTOCOL
Convert 450 ng of adapter -ligated DNA as per MethylCode Kit instructions (InvitrogenMECOV-50)
BISULFITE_CONVERSION_PERCENT
99.5873
LIBRARY_GENERATION_PCR_TEMPLATE_CONC
not measured
LIBRARY_GENERATION_PCR_POLYMERASE_TYPE
PfuTurbo Cx Hotstart DNA Polymerase, catalog #600410
LIBRARY_GENERATION_PCR_THERMOCYCLING_PROGRAM
2min 95C, 30s 98C, 10x (15s 98c, 30s 60C, 4min 72C), 10min 72C, hold 4C
LIBRARY_GENERATION_PCR_NUMBER_CYCLES
80
LIBRARY_GENERATION_PCR_F_PRIMER_SEQUENCE
standard Illumina TruSeq
LIBRARY_GENERATION_PCR_R_PRIMER_SEQUENCE
standard Illumina TruSeq
LIBRARY_GENERATION_PCR_PRIMER_CONC
undiluted Illumina TruSeq kit PCR primers
LIBRARY_GENERATION_PCR_PRODUCT_ISOLATION_PROTOCOL
2% high-resolution (APEX) agarose gel purification. Excise 250-600bp fragment, followed by MinElute-QG purification.
GEO Accession
GSM1463115