Whole forebrains were dissected, embedded in OCT, frozen at -80°C, cryo-sectioned at 16mm in the coronal plane onto PEN Membrane Glass Slides (Applied Biosystems, USA). Laser capture microdissection was performed using Leica DM LMD-6000 Laser Microdissection system to capture tissue containing the hippocampal CA1 ventricular lining. Total RNA was isolated from LCM-enriched samples pooled across ~6 slides per genotype using the Qiagen RNeasy Micro Kit and RNA quality was assessed using the Agilent Bioanalyzer 6000 Pico Kit [RNA Integrity Number (RIN) = 7.68±0.26 (mean±s.d.)]. SMART-Seq v4
GEO Accession
GSM3656140