In all experiments, total RNA was isolated from the samples using the SV Total RNA Isolation System (Promega, USA) and the quality assessment was performed using LabChip (PerkinElmer, USA). The rRNA was removed using Ribo-ZeroTM rRNA Removal Kit for Gram-negative Bacteria (Epicentre, USA). The sequencing library was prepared using the ScriptSeq-v2 RNA-Seq Library Preparation Kit (Epicentre, USA). Paired-end sequencing was performed on MiSeq (Illumina, USA) with the read length of 150 nucleotides.
GEO Accession
GSM4377785