Standard Trizol (Invitrogen) RNA extraction, per manufacturer's instructions. Additionally, 10 μg of purified hAGO proteins were subjected to Trizol RNA extraction. Barcoded small RNA sequencing was performed essentially as described (Hafner et al., 2011 (PMID 21775473)). RNA inputs of 2 μg (from T. ni cells) or 20 ng (AGO-isolated RNAs) were used per sample.
GEO Accession
GSM1167475