Cells were sorted into IMDM/10-20% FBS. Using the Chromium controller (10x Genomics) and reagents from the Chromium Single Cell 3′ Reagent Kit v2 (10x Genomics), cells were partitioned into nanoliter-scale Gel Beads-in-emulsion (GEMs) where each cell's trascriptome was indexed with a specific 10x BarCode. cDNA were amplified and purified for library preparation. cDNA were used for library preparation using the Chromium Single Cell 3′ Reagent Kit v2 (10x Genomics) according to manufacturer's protocol (CG00052, Rev D).
GEO Accession
GSM4458972