Total RNA was isolated from male adult flies about 1-2 days old using TRIzol. Poly(A) RNA was purified and fragmented, then incubated with Dynabeads coupled with m6A-specific antibody (202003, Synaptic Systems). The IP RNA fragments and untreated input control fragments are converted to final cDNA library in accordance with a strand-specific library preparation by dUTP method.
GEO Accession
GSM4709820