For single cell RNA sequencing, E16.5 cortex was dissociated into single cells at 37°C for 30 min with a Worthington Biochemical Papain Dissociation Kit (Cat# LK003150).  A Vi-CELL XR cell counter (Beckman Coulter) was used to determine the cell density and viability.  All samples had >90% viable cells.  Samples were processed using a Chromium Single Cell 3' Library and Gel bead kit v2 (10x Genomics) according to manufacturer instructions. cDNAs and libraries were prepared according to manufacturer instructions (10x Genomics).  Libraries were profiled by Bioanalyzer High Sensitivity DNA kit (Agilent Technologies) and quantified using a Kapa Library Quantification Kit (Kapa Biosystems).  Each library was sequenced in one lane of a HiSeq4000 (Illumina) per manufacturer recommendations (10x Genomics).
GEO Accession
GSM3037801