This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to ??1,500 samples per lane, and both PCR primers contain sequencer adapter regions.
experiment_title
AGP_freezethaw
target_subfragment
V4
sample_center
CCME
sequencing_meth
sequencing by synthesis
experiment_center
CCME
run_date
7/3/13
pcr_primers
FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
run_center
CCME
num_sequences
48691
platform
Illumina
library_construction_protocol
This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers F515 and R806 were developed against the V4 region of the 16S rRNA, both bacteria and archaea, which we determined would yield optimal community clustering with reads of this length The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to ??1,500 samples per lane, and both PCR primers contain sequencer adapter regions.
experiment_design_description
samples lost
study_center
CCME
target_gene
16S rRNA
row_number
11