Genomic DNA was extracted from 3 dnmt1+/+ and 3 dnmt1-/- zebrafish at 18 dpf using the DNeasy blood and tissue kit (Qiagen). 1mg and 0.5mg of DNA was used for bisulfite reactions and library construction using the TruSeq DNA PCR-free library preparation kit (Illumina) and the EpiGnome Methyl-Seq kit (Epicentre), respectively. The fragments were sequenced in paired-end 100bp mode on 1 lane of Illumina HiSeq 2500 instrument.
GEO Accession
GSM2607714