Pulverized frozen root tissue (2.5 mL) was thawed in 5 mL of PEB and a S16 fraction was obtained as detailed by Mustroph et al. 2009 PNAS.  RNA was extracted from 500µl of the S16 fraction using Trizol Poly(A)+ RNA-seq libraries were prepared from 15 µg of DNase I (Thermo Scientific) treated RNA extracted from the S16 fraction as described by Wang et al., PlosOne 2011
GEO Accession
GSM2601710