E16.5 midguts were digested in 0.25% Trypsin EDTA at 37°C for 30 minutes followed by neutralization with FBS. Cells were snap-frozen in liquid nitrogen and used for subsequent ChIP analysis. Whole cell lysates were sonicated and incubated with H3K4me1 antibody overnight (C15410194, Diagenode) for immunoprecipitation. ThruPLEX DNA-seq Kit (Rubicon Genomics) was used to prepare libraries, and 75 bp single-end reads were sequenced on an Illumina NextSeq 500 instrument.
GEO Accession
GSM3040279