50-500ng DNase-treated total RNA was used to construct libraries using Illumina TruSeq Stranded mRNA HT Sample  Prep Kit (Cat #RS-122-2103) with barcodes for sample multiplexing. The products were purified and enriched with PCR for 13 cycles to create final cDNA library. Libraries were quantified using HT DNA reagent kit (Part Number 760435, Caliper Life Sciences, Hopkinton, MA) using a LabChip GX (Perkin Elmer,Waltham,MA). Multiplexed libraries were equimolarly pooled, diluted to 2nM pool for final analysis on Agilent High sensitivity DNA kit to run on one rapid flowcell per lane and sequenced using 2x50 ntd paired end runs of the Illumina HiSeq 2500 platform.
GEO Accession
GSM2443049