Total DNA was extracted and purified using DNA extraction kit according to the manufacturer's instructions. Genomic DNA was digested using MspI restriction enzyme, then repaired the end and ligated with methylated adapters. The ligation products were purified and size-selected by agarose gel electrophoresis. Purified DNA from gel fragment was bisulfite-treated followed by PCR amplification.
GEO Accession
GSM1464587