The control and cigarette smoke exposed mice were first anesthetized with isoflurane and subsequently euthanized through cervical dislocation, and the lenses were extracted from mouse eyes and fiber cells were separated from the epithelium under a microscope as described previously. The extracted tissue was maintained in four distinct pools, each consisting of fiber cells from four mice eyes to serve as biological replicate for the control (CT-FC) and CS-exposed (CS-FC) mice. Upon extraction, lenses were dissolved immediately in Trizol reagent and total RNA was extracted for RNA-Seq library prepration. NEB Next Ultra RNA Library Prep Kit was used to prepare RNA-Seq libraries using total RNA isolated from control and cigarette smoke exposed mice.
GEO Accession
GSM4297024