Lysates were clarified from sonicated cells and histone-DNA complexes were enriched using PRDM15 antibody.  Antibody is an in-house produced rabbit polyclonal antibody against PRDM15. Directional RNA library was prepared according to Illumina's standard protocol. Detailed experimental procedures and bioinformatics analysis can be found in the supplemental method section of the paper.
GEO Accession
GSM3263559