Total RNA from mESCs was extracted using the QIAGEN RNeasy Mini kit with on-column DNase treatment following the manufacturer's protocol. Libraries were prepared using the Illumina TruSeq Stranded mRNA kit according to the manufacturer's protocol starting with 2 µg total RNA. Briefly, purified mRNAs (mRNA kit) were fragmented and converted to cDNA with reverse transcriptase. The resulting cDNAs were converted to double stranded cDNAs and subjected to end-repair, A-tailing, and adapter ligation. The constructed libraries were amplified using 8 cycles of PCR.
GEO Accession
GSM3774141