NA Poly A; Whole transcriptome library preparation was performed using 5-10 ng of fragmented enriched polyA RNA according to the manufacturer’s protocol (Ion Total RNA-Seq Kit V2 protocol #4476286B Life Technologies).  Size selection of a 315 bp product was performed using a standard Pippin prep protocol (Sage Science) followed by purification with AMPure beads (Beckman-Coulter Genomics).  The synthetic ERCC 1 and ERCC 2 control RNA library construction was performed directly from 30 ng of the non-polyA enriched sample.
GEO Accession
GSM1166058