The total RNA was isolated from cells using TRIzol Reagent (ThermoFisher, MA, USA) and purified using SV Total RNA Isolation System (Promega, WI, USA) according to the manufacturer's instructions. RNA samples were quantified using an ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE) and the quality was confirmed with a Tapestation (Agilent, CA, USA). The sequencing libraries were prepared from 1 ug of total RNA with NEBNext Ultra II Directional RNA Library Prep kit (New England Biolab, MA, USA) according to the manufacturer's instructions.
GEO Accession
GSM3964092