Brains (whole brains without the olfactory lobes) and spinal cords (1cm surrounding the lesion), were  harvested from uninjured lampreys, and at 10 time points from 6 hours to 12 weeks post injury. Tissue was stored in RNALater and then homogenized, pooled by time point (n=6 animals per time point), and total RNA (RIN >9) extracted using Trizol. RNA-Seq libraries (~250bp) were created using TruSeq RNA Sample Prep Kit v2 (Illumina Inc.; San Diego, CA), + Ribo-Zero Gold (Epicentre).
GEO Accession
GSM2521655