For RNA-seq, HSCs (Lin-, cKit+, Sca1+, CD150+, CD48-) and MPPs (Lin-, cKit+, Sca1+, CD150-, CD48-) were sorted from a pooled sample of 5 male and 5 female 8-week old mice. HSCs and MPPs were purified from three biological replicates (composed of pooled WBM from three male and three female mice) of either control or tests cohorts.  Total RNA was isolated from the samples using the NucleoSpin RNA XS kit. The SMARTer Ultra Low RNA kit (Clontech) was used to prepare the libraries from 3-5ng of total RNA.
GEO Accession
GSM3405439