Villus chromatin digested with MNase was incubated overnight with 5 ul H3K27ac antibody (Active Motif, 39135) for immunoprecipitation. ThruPLEX DNA-seq Kit (Rubicon Genomics) was used to prepare libraries, and 75 bp single-end reads were sequenced on an Illumina NextSeq 500 instrument.
GEO Accession
GSM2610654