Vegetative cells were harvested, freed from bacteria and incubated for 24 h on non-nutrient agar until fruiting bodies had formed. Dictyostelium lacteum amoebas were co-cultured with Klebsiella aerogenes on lactose-peptone agar. RNeasy kit (QIAGEN) was used for extraction. Libraries were prepared with Illumina Truseq stranded mRNA kit.
Experimental Factor: cell type
Vegetative cell
Experimental Factor: time
24