Fish was sampled at freshwater stage after initial feeding and anesthesized by MS-222 buffered with same amount of biocarbonate. Fish was immediately dissected under dissecting microscrope, tissues were then put into RNAlater. The tissues were stored under 4 degree for 24h before moved to -80 for longterm storage.   Fish was given either vegetable oil diet low in EPA and DHA, or fish oil diet high in DHA and EPA, or a phospholipid diets high in EPA and DHA in phospholipids.   Total RNA was extracted by using QiaGen RNeasy Plus Universal Kits. RNA quality was checked by using Agilent Bioanalyzer 2100.   Library preperation was done by using TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA).
Experimental Factor: strain
wild
Experimental Factor: age
94
Experimental Factor: organism part
liver
Experimental Factor: diet
phospholipid feed