Embryo pools underwent RNA extraction and amplification using RNaqueous Micro (Life Technologies) and subsequently amplified using the MessageAmp II aRNA amplification kit (Life Technologies). Libraries of amplified RNA for each pool were prepared following the Illumina mRNA-Seq protocol. Sequencing libraries were created from 50 ng samples and sequenced with Illumina’s HiSeq 2000 at the University of Wisconsin-Madison Biotechnology Center.
GEO Accession
GSM1169953