For M5C-seq, total RNA were extracted with Qiagen RNeasy Midi Kit and RNA were isolated with antibody. For RIP-seq, tissues were lysated with 1X polysome lysis buffer and RNA-protrein complexes were isolated with antibody. Libraries were prepared according to TAKARA SMARTer RNA-Seq Pico Input kit M5C-seq/RIP-seq
GEO Accession
GSM4147089