Spinal cord microglia were isolated via a Percoll gradient Sequencing was performed by the High Throughput Genomics Group at the Wellcome Trust Centre for Human Genetics (Oxford University). RNA libraries were prepared using a SMARTer Ultra Low Input HV kit (634820, Clontech). Custom adaptor and barcode tags were used (5'- P-GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG, 5’-ACACTCTTTCCCTACACGACGCTCTTCCGATCT). Samples were amplified (13 cycles for RNA) and multiplexed in replicate flow cells on an Illumina HiSeq2500 platform to yield 50bp paired-end reads at a depth of at least 20M. All libraries were prepared together to avoid batch effects.
GEO Accession
GSM1827985