Total RNA was isolated using a RNeasy Mini kit and treated with DNase (Qiagen). Chromatin was isolated, sonicated to 200-300 bp in length, and subjected to immunoprecipitation overnight with respective antibodies. The ChIP DNA was eluted, decrosslinked, and purified. mRNA-seq libraries were prepared with a KAPA mRNA Hyper Prep kit. ChIP-seq libraries were prepared with a ThruPlex DNA-seq kit.
GEO Accession
GSM4147897