RNA was extracted with Trizol reagent. RNA quality and quantity were first determined with the Agilent Bioanalyzer 2100, accepting RNA integrity numbers (RIN) of >7 and quantities of 100 nanograms or more per sample.  Samples were enriched for mRNA using oligo-dT columns. RNA libraries were prepared for sequencing using standard Illumina TruSeq protocols
GEO Accession
GSM3042001