Lungs were lavaged with 2x 1mL of saline and then removed. The left lung was placed into RNAlater (Qiagen) and stored at 4 degrees Celcius for 24 hours after which time it was flash frozen in liquid nitrogen and stored at -80 degrees celcius. cDNA libraries were constructed for sequencing using a KAPA Stranded Total RNA Kit (Kapa Biosystems)
GEO Accession
GSM3265421