Pelleted cells were lysed with Proteinase K and 20% SDS. Nucleic acids were extracted using phenol:chloroform:isoamyl alcohol and phase separated using phase lock tubes. Samples were then digested using restriction enzyme cocktails then treated with sodium bisulfite in a non-denaturing way. SMRTbell libraries were constructed using Pacific Bioscience's amplicon template preparation protocol.
GEO Accession
GSM3772632