Total RNA was extracted with the Trizol reagent (Invitrogen, Beijing, China) based on the supplier’s instructions The ribosomal RNA was removed from total RNA (~2 µg) with RiboZeroTM Magnetic Kit (EpiCentre, Cleveland, OH). The processed RNA samples were used to prepare double-stranded complementary DNA (cDNA) libraries with the M-MuLV Reverse Transcriptase and NEBNext® mRNA Second Strand Synthesis Module. The cDNA molecules were fragmented by sonication, and further processed for end repair, adaptor attachment, and sequence amplification.
GEO Accession
GSM2522412