Cells were freed from bacteria and incubated at 2.5 x 10^6 cells/ml in 250 mM sorbitol in 20 mM K-phosphate to induce encystation. Total RNA was extracted directly after harvest (t=0 h) and after 8, 16 and 24 h of incubation at 22C. Polysphondylium pallidum PN500 was co-cultured with K. aerogenes on LP agar. RNeasy kit (QIAGEN) was used for extraction. Libraries were prepared with Illumina Truseq kit with PhiX reference spike-ins.
Experimental Factor: cell type
Cyst
Experimental Factor: time
0