Total RNA was prepared from the neutrophil solutions using the RNeasy Mini Kit as per manufacturer instructions (Qiagen, Cat. no. 74104). The RNA quantity and quality were determined using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Samples with an RNA Integrity Number (RIN) < 7 or a yield < 10 μg were excluded from the experiment. Sequencing libraries were prepared using the Illumina TruSeq RNA Sample Preparation kit according to the manufacturer protocol (Illumina Inc., USA).
GEO Accession
GSM4456513