Extraction of total RNA from primary mouse lung endothelial cells was performed using the RNeasy Mini Kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions.  DNA was eliminated by on-column RNase-free DNase treatment.  RNA quality was verified (RNA integrity number >9) using a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). Messenger RNA was isolated, converted to cDNA, and amplified using the mRNA Purification Kit (Illumina, San-Diego, CA) according to the manufacturer’s instructions. An Illumina library was built and used to generate clusters on the Illumina flow cell according to the Illumina protocol and sequenced using a HiSeq 2000 (Illumina).
GEO Accession
GSM1828657