Cardiac ventricles were harvested at P1 and P14 (n=9 per group) from C57BL6/J mice as described above and were homogenised in PBS. Three individual hearts were pooled per replicate. RNA was isolated by TRIzol extraction (Ambion) followed by QiaQuick MinElute cleanup including on-column DNase digestion. RNA quality was verified with MultiNA bioanalyzer (Shimadzu, Kyoto). Purified mRNA was prepared using Dynabeads® Oligo (dT)25 (Ambion) enrichment. Libraries were generated using the NEBNext® mRNA Library Prep Reagent Set for Illumina (New England Biolabs). Libraries were validated on MultiNA bioanalyzer and sequenced at a concentration of 10 pM on Genome Analyzer IIx instrument for 36 cycles. Illumina base-calling was performed off-line with OLB v1.8.
GEO Accession
GSM1462886