150 mg of ground pelleted cells was resuspended in ChIP lysis Buffer. Sonication with Bioruptor (Diogenode) with 50 cycles of pulses of 30s ON/OFF to obtain 100-300 bp fragments. Clarification by centrifugation. Immunoprecipitation with Red Fluorescent Protein (RFP)-Trap Magnetic Agarose (MA) beads (Chromotek) to the IP samples and with MA beads (Chromotek) to the binding control. Washing and de-crosslinking. RNase A and Proteinase K treatment. DNA purification by phenol:chloroform TruSeq ChIP Library Prep Kit
GEO Accession
GSM3886386