Oocytes were exposed to solution with 80IU hyaluronidase (Sigma, USA) and manipulated with pipettes to remove cumulus cells. Then oocyte were rinsed in Ca2+/Mg2+ free PBS solution and individually placed in 0.2 ml PCR tubes containing 10μl Invitrogen TRIzol. The samples was stored at -80℃ pending further processing until RNA was isolated with the E.Z.N.A. ®Total RNA kit ‖ ( (OMEGA, USA). RNA from single oocyte was amplified by Smart-seq2
GEO Accession
GSM4696895