Total RNA from liver tissue was extracted by using a mirVana miRNA extraction Kit (Ambion, Austin, TX, USA) according to the manufacturer's instruction. 1) Filter Small RNA: Separate RNA segment of different size by PAGE gel,select 18-30nt stripe and recycle. 2) Adaptor  ligation: Prepare connection system, blend and centrifuge, connect warmly for a period of time. 3) RT-PCR: Prepare  reverse transcription system, connect warmly for a period on PCR on PCR instrument, then PCR amplification. 4) Purify  PCR products: Recover the Purified PCR construct by PAGE gel, dissolve the recylced products in EB solution. Finally,  label to finish the library construction. 5) Library quality testing: Use Agilent 2100 Bioanalyzer and ABI StepOnePlus Real-  TimePCR System to test quality and production of the prepared library.
GEO Accession
GSM4647598