Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood using a standard Ficoll-Paque density gradient centrifugation method.  Total RNA was isolated using the RNeasy Miniprep Kit (QIAGEN) per the manufacturer's instructions. Total RNA from each sample was converted to sequencing libraries using the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Clontech), following the manufacturer's protocol.
GEO Accession
GSM3405492