Testes were dissociated by enzymatic digestion and vigorous agitation. GFP-expressing somatic cells were purified by FACS using a Sony SY3200 highly automated parallel sorting (HAPS) cell sorter into 750 µL TRIzol LS (Thermo Fisher). RNA was isolated and precipitated according to manufacturer's instructions. RNA extracts were cleaned using RNeasy Mini Kit (QIAGEN) according to manufacturer's instructions. RNA quality and quantity were determined using the Agilent 1200 Bioanalyzer. The RNA sequencing was performed by the Genome Technology Center at the NYU Langone Medical Center using Illumina HiSeq2500 Paired-End 50 Cycle Flow Cell.
GEO Accession
GSM4456439