All isolated cells (Hepa1-6_Tu and Py8119_Tu, G-MDSCs) were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. The DNA of G-MDSCs were extracted using a DNA extraction kit (56504, QIAamp DNA Investigator Kit). Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used to construct the libraries of mRNA-seq. TrueMethyl Seq protocol recommended by Cambridge Epigenetix (CEGX, Cambridge, UK) was used to prepare the libraries for oxidative bisulfite sequencing (OxBS-seq).
GEO Accession
GSM4296350