Immunomagnetic Enrichment and Fluorescence Activated Cell Sorting (IE/FACS) cells were spiked into phosphate buffered saline (PBS) and peripheral blood (PB) from healthy female donors. RNA extracted from cells was polyA+ labelled and used to generate Helicos DGE or Illumina libraries   following the manufacturer’s protocols.  The modified cDNA molecules were subjected to sequencing by the Helicos Heliscope or Illumina Hiseq 2000 system
GEO Accession
GSM1829608