the BLISS protocol described in Yan et al, 2017 was followed. Paraformaldehyde fixed cells on coverslips were permeabilised and DNA free ends were blunted prior to ligation in situ of oligonucleotides adapters. Genomic DNA is isolated from cells by phenol:chloroform purification protocol and sonicated to achieve a mean fragment size of 300-500bp. Genomic DNA purified with AMPure beads is used as template in In Vitro Transcription reaction. RNA is isolated from IVT reaction using agencourt RNAClean XP beads after DNAseI treatment. libraries were prepared from RNA with TruSeq smallRNA Library Prep Kit (Illumina)
GEO Accession
GSM4520696