Total RNA was isolated from sorted cells using the RNeasy Micro Kit (Qiagen 74004). cDNA was synthesized from RNA samples with the Ovation RNA-Seq System V2 kit (NuGEN) according to manufacturer's instructions. RNA-seq libraries were analyzed by Bioanalyzer (Agilent) and quantified by qPCR (KAPA). Sequencing was carried out on an Illumina HiSeq 4000 using 100-bp paired end reads. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM4709469