50,000 cells from each sample were washed in cold PBS, lysed, and transposed for 40 minutes at 37°C using Nextera Tn5 transposase enzyme (Illumina). Transposed DNA fragments were amplified 10-15 cycles and barcoded using Illumina compatible indexed primers purchased from IDTdna.com. Library purification was performed with the MinElute PCR Purification Kit (Qiagen) and size selection was using AMPureXP beads (Beckman Coulter). Libraries were quantified and size distribution was assessed using the Bioanalyzer High Sensitivity DNA Kit (Agilent).
GEO Accession
GSM4697392