Genomic DNA was extracted with the Nucleon™ BACC Genomic DNA Extraction Kit (GE Healthcare, Life Sciences). Reduced Representation Bisulfite Sequencing libraries were constructed as previously described (Boyle et al., 2012, Gel-free multiplexed reduced representation bisulfite sequencing for large-scale DNA methylation profiling. Genome biology 13, R92). Briefly, 200 ng of genomic DNA was digested with 40 U of MspI enzymes (New England Biolabs) and ligated to TruSeq (Illumina) sequencing adaptors. Bisulfite conversion was conducted once with the EpiTecht bisulfite kit (Qiagen) in accordance with the manufacturer’s instructions, and the converted DNA was amplified by PCR and sequenced on a HiSeq Illumina instrument.
GEO Accession
GSM2299456