Total RNA was extracted from mouse uterus ( three mice per genotype) and ishikawa cells (duplicate per phenotype) using the mirVana miRNA isolation kit. RNA quality control was performed on an Agilent 2100 bioanalyzer Poly-A RNA libraries were constructed using mRNA Stranded TruSeq protocol (Illumina, San Diego, CA). Small RNA library construction was performed using the TruSeq Small RNA kit (Illumina). Purified libraries were visualized and quantified using a TapeStation HSD1000 (Agilent, Santa Clara, CA).
GEO Accession
GSM4709385