Excess dead cells were depleted from samples using bead-based Dead Cell Removal Kit (#130-090-101, MACS miltenyi Biotech) Cells were combined with reverse transcriptase Master Mix and partitioned into Gel Bead-In EMulsions (GEMs) using 10X GemCode Technology, where the poly-A transcripts are barcoded with an Illumina R1 sequence, a 16 bp 10X barcode and a 10 bp Unique Molecular Identifier (UMI). 11-12 cycles of PCR was used to amplify the cDNA.
GEO Accession
GSM4455929