Material collection: ZT8. ChIP protocol using anti-GFP Ab (Abcam #ab290) Libraries were prepared according to manufacture's instructions of the ChIP-seq sample prep kit from Illumina. Briefly, DNA was end-repaired using a combination of T4 DNA polymerase, E. coli DNA Pol I large fragment (Klenow polymerase) and T4 polynucleotide kinase. The blunt, phosphorylated ends were treated with Klenow fragment (3’ to 5’ exo minus) and dATP to yield a protruding 3- 'A' base for ligation of Illumina's adapters which have a single 'T' base overhang at the 3’ end. After adapter ligation, DNA from 150-400 bp was band isolated from an agarose gel. The purified DNA was PCR amplified with Illumina primers for 18 cycles and captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Genome Analyzer II following the manufacturer's protocols.
GEO Accession
GSM1167546