The bacteria were cultured in 5ml brain heart infusion (BHI) broth (Becton Dickinson and Company, le Pont de Claix, France) containing 5% fetal calf serum (FCS; Biochrom KG, Berlin, Germany) overnight until OD600nm = 0.4 Ribosomal RNA was depleted using Ribominus (Invitrogen) and RNA purification was carried out with RNA Clean & Concentration™-5 kit (Zymo Research) following the manufacturers' instructions and purified RNA was eluted in 6 μl TruSeq® Stranded mRNA (Illumina) was used to prepare libraries
Experimental Factor: stimulus
none
Experimental Factor: genotype
wild type genotype