Sorted satellite cells were directly processed for ATAC-seq library preparation without in vitro culture. ATAC-seq was performed according to a published protocol (Buenrostro et al., 2013) with minor modification. 2000-5000 cells were processed for each sample and treated with the Tn5 transposase (Illumina) for 30 min at 37°C and PCR-purified (Qiagen) to generate library fragments. ATAC seq data were generated on Illumina HiSeq 2500 instruments according to the manufacturer's instructions.
GEO Accession
GSM4709355