mRNA was extracted from SCN tissue using a Dynabead mRNA DIRECT Micro Kit (Ambion) according to the manufacturer's instructions. All mRNA samples were examined for quantity and quality by NanoDrop and Bioanalyzer (Agilent). Samples were randomized for library preparation and sequencing. Libraries were constructed following Truseq protocols (Illumina) and sequencing was performed at the McDermott Sequencing Core at UTSW.
GEO Accession
GSM4458739