Trizol-stored blastomeres were thawed and re-frozen up to three cycles to lyse them. RNA extraction was performed using a Trizol/chloroform extraction followed by RNeasy Micro (Qiagen) preparation using On Column DNaseI Digestion (Qiagen). mRNA was amplified using in vitro transcription (TargetAmp aRNA Amplification Kit, Epicentre). Standard RNA-seq protocol  (Illumina mRNA Sequencing Sample Preparation Guide, September 2009) was used to sequence amplified aRNA. Magna beads were used for final purification in lieu of gel purification.
GEO Accession
GSM1462556