1 million cells were pelleted and washed once with sterile PBS, then resuspended in 350 ul RNALater (Qiagen). RNA was isolated using a Qiagen RNeasy Plus mini kit and eluted in 30 microliters RNA-free water. NEBNext_dual rRNA-depleted stranded (HMR) libraries were prepared for sequencing with standard Illumina protocols using the services of Genome Quebec
GEO Accession
GSM3888537