ChIP experiment was performed with EpiTect ChIP OneDay Kit (Qiagen) according to the manufacturer’s protocol and H3K27me3 antibody (07-449, Millipore) Libraries were prepared using Illumina TruSeq ChIP Sample Prep Kit (Illumina) with immune-precipitated DNA for each sample. The sizes of the libraries were validated utilizing the DNA 1000 chip on the Agilent 2100 Bioanalyzer. The quantification of the libraries was done using the KAPA Library Quantification Kit.
GEO Accession
GSM2091491