RNA isolation was performed with the miRNeasy mini kit (Qiagen) following manufacturers instruction. To remove the feeders, cells were pre-plated for 30 minutes before RNA processing. RNA was eluted from the columns using RNase-free water and quantified by Nanodrop. cDNA was produced with the High Capacity RNA-to-cDNA kit (Applied Biosystem). Libraries were prepared using the SMARTer Universal Low Input RNA Kit (Clontech Laboratories) according to the manufacturer's protocol.
GEO Accession
GSM1830141