Total RNA was harvested from 100zebrafish embryos and >20 million mouse NPCs for each replicate using Trizol reagent. One microgram of total RNA was used for the construction of both RNA and small RNA sequencing libraries. RNA libraries were prepared for sequencing using standard manufacturers' protocols (Illumina TruSeq kit for RNA-Seq libraries and NEBNext Multiplex small RNA library kit for small RNA libraries).
GEO Accession
GSM2610413