Leaves were cut off and directly frozen in liquid nitrogen Arabidopsis thaliana plants were grown from seeds on solid MS medium. Plants were kept under axenic conditions and grown under 12 h of light (80-100 µE) at 23 °C, 12 hours of dark at 20 °C, 70-80% relative humidity for 9 days Roots of 100 9-day-old A. thaliana plants were inoculated with cellophane strips containing mycelium of L. bicolor. Ten control plants were mock-treated with cellophane strips without without the fungi. Two days post treatment, leaves which were not in contact with the fungi were harvested and shock frozen in liquid nitrogen RNA was extracted using RNAeasy extraction kit (Qiagen) according to the manufacturer's protocol Libraries were constructed using the TrueSeq RNA Library Prep Kit (Illumina) according to the manufacturer's protocol
Experimental Factor: infect
Laccaria bicolor