Animals were transcardially perfused with PBS and the liver was excised. Single-cell suspensions of liver cells were obtained by mechanically disrupting harvested livers and filtering through 70-μm mesh filters (Thermo Fisher Scientific). Ammonium-chloride potassium (ACK) lysis was performed to remove erythrocytes prior to cell counting. For fluorescence-activated cell sorting (FACS), samples were stained for CD45 and CD4 underwent FACS using BD Influx achieving 95% purity. FACS-purified samples were collected into PBS buffer containing 2% (v/v) BSA. Samples were tested for viability (all samples >82%) and 6000 cells were loade according to the 10X 5' user guide. 3 NextSeq steps were performed (NextSeq High Output 150-cycle v2.5) Droplet-based single cell RNA sequencing
GEO Accession
GSM4647712