Sampling for transcriptome analysis from chemostat cultures and total RNA extraction was performed as described previously (Hazelwood et a., Appl. Environ. Microbiol. 76:318-328). cDNA libraries for the Illumina platform were generated and sequenced at BaseClear BV (Leiden, The Netherlands). cDNA was used as input for library preparation using the Illumina TruSeq DNA library preparation kit (Illumina). Briefly, the DNA was fragmented by nebulization and subjected to end-repair, A-tailing, ligation of adaptors including sample-specific barcodes and size-selection to obtain a library with median insert-size around 200-400 bp. After PCR enrichment, the resultant library was checked on a Bioanalyzer (Agilent) and quantified. The libraries were multiplexed, clustered, and sequenced on an Illumina HiSeq 2500 with paired-end protocol library with a 350 insert size were prepared and sequenced with an Illumina Hiseq 2500 sequencer.
GEO Accession
GSM1446829