Small RNAs were purified using the mirVana™ miRNA isolation kit (Ambion) 21-30nt small RNAs were size-selected on a 17.5% polyacrylamide/7M urea gel and ligated to a 3' adapter. Ligated species were size-selected on a 17.5% polyacrylamide/7M urea gel and ligated to a 5' adapter. RNA was reverse transcripted into cDNA and PCR-amplified in a two-step process. Amplified cDNA was gel-purified.
GEO Accession
GSM3041243