Whole pancreas tissue was stored in RNAlater (Qiagen) and then frozen at -80C. RNA was extracted using the Qiagen Rneasy Mini kit. The TempO-Seq® Assay User Guide was used to build the TempO-Seq libraries for the mouse whole transcription panel. Following amplification, libraries were pooled and purified using the Macherey-Nagel NucleoSpin Gel and PCR Cleanup Kit with modifications outlined in the TempO-Seq® User Guide. Pooled, purified libraires were quantified using the KAPA Biosystemd qPCR library quantification kit and diluted to 4 nM for denaturation and dilution for sequencing. Human TempO-Seq® mouse whole transcription panel and libraries were sequenced using an Illumina NextSeq500.
GEO Accession
GSM4296488