Cells were lysed with RLT-Plus lysis buffer containing beta-mercaptoethanol, and lysated were processed according to the protocol of the QIAGEN's RNEasy Plus Mini kit. RNA with RIN of 9 or higher was used for library preparation. RNA library was created in accordance with the TruSeqTM RNA Sample Preparation v2 Guide (Illumina, San Diego, CA, USA), using the PerkinElmer Sciclone liquid handler, resulting in 330bp cDNA fragments.
GEO Accession
GSM1828768