Total RNA was isolated using the miRNeasy kit (QIAGEN) according to the manufacturer's instructions. Ribosomal RNA was depleted from total RNA using the Ribo-Zero Gold Kit (Epicentre, Madison, WI). Directional cDNA libraries were prepared using a KAPA Biosystems Stranded RNA-seq Library Preparation kit, pooled, and sequenced on an Illumina HiSeq 2500 platform across multiple lanes.
GEO Accession
GSM4146878