RNA was extracted using QIAGEN RNeasy micro kit (catalog number: 74004) according to manufacturer's instructions RNA-Seq  cKIT sorted rhesus PGCs, rhesus liver cells, and rhesus iPS cells were lysed in 350ul RLT buffer (Qiagen) and RNA was extracted using the RNeasy Micro Kit (Qiagen) according to manufacturer’s instructions. RNA was amplified and converted to cDNA using the Ovation RNA-Seq System V2 (Nugen). cDNA was sonicated to DNA fragments within the 200bp range using a Covaris S2 sonicator according to manufacturers instructions (Duty cycle 10%, Intensity 5, Cycles per burst 200, and time 180 sec). Subsequently libraries were generated using the Encore Rapid Library System according to manufacturers instructions. Library concentration was estimated using a Kapa library quantification kit (Cat# 4827) according to manufacturers instructions.  Rhesus Macaque PGC and embryonic liver libraries were run using 100bp paired-end reads on the HiSeq 2000 System (Illumina). The rhesus iPSC libraries were run using 50bp single end read.  ERCC RNA Spike-In Mix (Cat# 4456740, Invitrogen) was used according to manufacturer’s instructions in the liver cells and iPSC samples.
GEO Accession
GSM2522966