Single cell suspensions were generated using accutase dissociation followed by single cell filtration of iPSC-derived cortical neurons. The single cell suspension was sorted into a 96-well PCR plate containing a lysis mix. Sorting gates were set to include only live (DAPI negative) single cells. Stream alignment and sort efficiency was checked using Accudrop beads (Becton Dickinson). Single iPSC derived cortical neurons were isolated by FACS onto 96-well plates in 2ul lysis buffer. Libraries were prepared following the Smart-Seq2 protocol. Libraries were pooled in 288- or 384-plexes and each pool sequenced on 1 lane of HiSeq4000 at 75bp paired end
GEO Accession
GSM3345121