Approximately 10^7 cells were lysed directly on plate with 1 ml TRIzol (Invitrogen). RNA extraction was performed following manufacturer protocol. For RNA-seq library preparation, approximately 1 μg of TRIzol (Invitrogen) isolated total RNA from mESC cells was subjected to ribosomal RNAs depletion using Ribo-Zero Gold kit (Epicentre). rRNAs-depleted RNA was used as input for RNA-seq library preparation using the TruSeq RNA Sample Prep Kit (Illumina), following manufacturer instructions
GEO Accession
GSM1464628