Total RNA extracted using TRIzol  from 100 mg of root tissue dsRNA sequencing (dsRNA-seq) libraries were prepared as previously described  (Zheng et al., 2010 PLos Biol)  . Briefly, total RNA was subjected to two rounds of rRNA depletion (Ribominus, Invitrogen (Carlsbad, CA)), and then treated with a single-strand specific ribonuclease (RNase One, Promega (Madison, WI)) per manufacturer’s instructions. The RNA samples were then used as the substrate for sequencing library construction using the Small RNA Sample Prep v1.5 kit (Illumina, San Diego, CA) as per manufacturer’s instructions.
GEO Accession
GSM2601713