Reverse transcription of viral RNA, followed by demi-hemi PCR of 4 amplicons with serotype-specific primers. Pooling of PCR products and creation of 454 libraries.
analysis_type
RNA Viral 454 Alignment and Assembly
gssr_id
39723.0
lsid
broadinstitute.org:bsp.prod.sample:WAFP
material_type
DNA:ss Viral cDNA
primary_disease
Dengue
project
V3232
root_sample_id
SM-OHA3
sample_id
SM-WAFP
work_request
26154