OSA tumor and non-tumor tissue was obtained from a total four non-malignant bone and three osteosarcoma specimens via veterinary surgeons following amputation treatment or immediately following euthanasia. Confirmation of OSA was confirmed by pathologists via histopathological examination. A 1cm3 piece of tumor was extracted along with a 1cm3 piece of adjacent non-tumor affected bone tissue. These samples were placed directly into RNAlater® and shipped at room temperature and stored at -20°C. OSA tumor and matched adjacent non-tumor bone tissue samples were homogenized. and RNA extracted using Qiagen RNeasy Mini Kit (Qiagen, Germany) according to manufacturer's instructions. The extracted RNA was stored at -80°C. RNA was quantified using both a NanoDrop 8000 (Thermo Fisher Scientific, USA), and an Agilent 2100 Bioanalyzer (Agilent, USA). RNAseq was completed at Edinburgh Genomics using an Illumina HiSeq platform. In all cases >20 million reads were obtained from all samples analysed. Illumina TruSeq stranded library prepartion for paired end sequencing.
GEO Accession
GSM4709435