Mouse brains were flash frozen in isopentane, frontal cortex was isolated and homogenized, followed by total RNA isolation via Tri Reagent. RNA was sent to the Centre for Applied Genomics in Toronto, Canada for poly-A isolation, library generation, amplification, and sequencing. Protocol according to The Centre for Applied Genomics (TCAG, Toronto, ON, CAN)
GEO Accession
GSM2610724