Cryopreserved perhipheral blood mononuclear cells were thawed and CD34+ lineage negative cells isolated by FACS for single cell RNA sequencing using the 10x Genomics 3' single cell platform. Libraries were prepared as per the 10x Genomics Chromium Single Cell 3' Reagent kit user guide. Samples 1-16 were done with version 2 of 10x Genomics Chromium Single Cell 3' Reagent kit and Samples 17-21 were done with version 1 of 10x Genomics Chromium Single Cell 3' Reagent kit.
GEO Accession
GSM4296491