Nuclei from 50,000 cells were used in transposition reactions using the Nextera DNA Library Prep Kit (Buenrostro et al, 2013). DNA was purified using the Qiagen MinElute kit. Libraries were amplified with indexed primers using NEBNext High-Fidelity PCR master mix and quantified by qPCR using KAPA Illumina Standards.
GEO Accession
GSM4709973