GSM4210459: Lymphoma, Bptf.WT.Early (PTB312); Mus musculus; RNA-Seq
Lymphoma samples were removed and flash frozen. Total RNA from lymphoma samples was isolated using guanidine thiocyanate, followed by acid phenol–chloroform extraction. PolyA+ RNA was extracted and randomly fragmented, converted to double stranded cDNA and processed through subsequent enzymatic treatments of end-repair, dA-tailing and ligation to adapters according to Illumina's 'TruSeq RNA Sample Preparation Guide'. The adaptor-ligated library was completed by limited-cycle PCR with Illumina PE primers (10 cycles, or 8 cycles for samples using the v.2 protocol). The resulting purified cDNA library was applied to an Illumina flow cell for cluster generation (TruSeq cluster generation kit v.5) and sequenced on the Genome Analyzer IIx with SBS TruSeq v.5 reagents, according to the manufacturer's protocols.
GEO Accession
GSM4210459