Pooled sample consisting of samples: Cyst 910 S1|Cyst 974 S1|Cyst 903 S1|Cyst 911 S1|Cyst 927 S1|Cyst 936 S1|Cyst 992 S1|Cyst 905 S1|Cyst 929 S1|Cyst 937 S1|Cyst 922 S1|Cyst 915 S1|Cyst 931 S1|Cyst 939 S1|Cyst 987 S1
Primers were used to amplify DNA in 25-uL multiplex polymerase chain reactions (PCRs) as described previously 15.  For each sample, three multiplex PCRs were performed, with each multiplex PCR containing 22 to 50 primer pairs.  Reactions were purified with AMPure XP beads (Beckman Coulter) and eluted in 100 uL of Buffer EB (Qiagen, cat. no. 19086).  0.25% of the purified PCR products were then amplified in a second round of PCR, as described in Springer et al.15.  The PCR products were purified with AMPure and used for sequencing on a MiSeq or HiSeq instrument