Subject M1 cortex
Immunoglobulin (Ig) variable region heavy and light chain libraries were constructed from an autopsy sample of a human subject with clinically defined multiple sclerosis. Separate libraries were generated for two non-consecutive sections of the cortex. RNA was reverse transcribed into cDNA and amplified by PCR as previously described (Willis et al, 2009. J Immunol 182(5):3310–3317). A proxy Phred quality score of Q=30 was been assigned to all positions.