Embryonic transcriptome of a mix of embryos of different developmental stages
Librarypreparation was conducted according to the Illumina standardRNAseq protocol. Half of one Illumina HiSeq2000 lane resulted in221,218,272 paired-end 101 bp reads. Low quality bases and PolyA/Tstretches longer than ten bps were trimmed using custom perlscripts. The initial dataset was split randomly into two sub-datasets.The first dataset was assembled using Velvet(version 1.2.08) and Oases ( version 0.2.08) with ak-mer size of 27. Assembled transcripts were then used as input(-long flag) for the second assembly. This resulted in 421,361assembled sequences (470,016,133 bp). The N50 of the assembly is2275 bp.