GSM3267438: Mouse LGN Fn14 WT rep 2; Mus musculus; RNA-Seq
Mice were euthanized at P27. The LGNs of 4 WT and 4 KO mice were included in the analysis, and each mouse was processed as an independent bioreplicate.  After isoflurane anesthetization, mice were decapitated and the brain was isolated. Mouse brains were dissected and 300 um coronal sections were made on a Leica VT1000S vibratome. The dorsal LGNs were then microdissected in ice cold PBS after visual identification using a Nikon SMZ-10A brightfield dissection microscope, and flash frozen. Total RNA was trizol-extracted and purified using the Qiagen RNeasy Micro kit. After RNA extraction, total RNA was depleted of ribosomal RNA, heat-fragmented to ~350 bp using the NEBNext kit, and processed with adapters to allow nonspecific sequencing on an Illumina NextSeq 500 System.
GEO Accession
GSM3267438