GSM2188074: 2015-423; Trichoderma reesei; RNA-Seq
Frozen mycelia were disrupted and homogenized in Lysis Matrix C (Fast RNA Pro Red Kit, MP Bio) in the presence of 700 µL of Lysis buffer RLT (RNeasy Mini Kit, Qiagen) complemented with 7 µL of β-mercaptoethanol on a rotor-stator homogenizer (FastPrep, MP Bio, Santa Ana, CA, USA). After centrifugation, the lysate was transferred on a Qiashredder spin column (Qiagen) and centrifuged for 2 min to eliminate all cell debris. The flow-through was then mixed with 0.5 volumes of pure ethanol and transferred to an RNeasy spin column. All further steps, including an on-column DNase digestion, were realized following the manufacturer’s instructions. The quantity and quality of the total RNA were determined using a Nanodrop spectrophotometer (Nanodrop tech- nologies, Wilmington, DE) and the Bioanalyzer 2100 system (Agilent, Santa Clara, CA) TruSeq Stranded mRNA Illumina
GEO Accession
GSM2188074