GSM4210260: PP1_WingDisc_G1; Drosophila melanogaster; OTHER
Tissue was disrupted by douncing and filtered over a 30 mM filter to collect follicle cell nuclei. DAPI-stained nuclei were sorted by 5-Laser FACS Aria III and G1 and S-phase populations were collected. Genomic DNA was fragmented by sonication and extracted by using a standard Phenol-Chloroform extraction protocol and precipitated with sodium acetate, glycogen, and isopropanol. Libraries were prepared according to the protocol accompanying Rubicon Thruplex DNA-seq kit (R400427).
GEO Accession
GSM4210260