GSM1835557: GM4B; Homo sapiens; OTHER
Antibody conjugates bearing a cleavable covalently attached anchor oligonucleotides were generated by commercially available hydrazine-carbonyl conjugation chemistry.  Synthetic signature oligonucleotides comprised of all necessary Illumina adapter sequences and an extended barcode were hybridized to the universal anchor DNA sequence. Following cleavage and isolation of the oligonucleotide complexes, libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit.  Following preamplification, the final purified products are quantitated by qPCR before cluster generation and sequencing.
GEO Accession
GSM1835557