Illumina HiSeq 2000 paired end sequencing
This experiment was intended to identify the role of intestinal microbiota of in the pathogenesis of Non-Alchoholic Fatty Liver Disease (NAFLD)
DNA was extracted from fecal samples using the QIAamp DNA mini stool kit (Qiagen, Courtaboeuf, France), according to the manufacturers protocol with the addition of a bead-beating step (<106�m diameter beads, 6500 rpm, 3x30 seconds).The library was prepared from 3 �g of extracted DNA using the Bioscientific PCR free library kit according to the manufacturers instructions. Following sequencing, host contamination was removed from the library as required by patient consenting agreements by alignment against the Homo sapiens genome (GRCh38) using bwa 0.7.12 and discarding any aligned reads.