GSM1178898: H2AZ_WT26_ZT18; Mus musculus; ChIP-Seq
Adult mouse liver were collected at 6 different time of the day (mouse held under LD12:12, time points every 4hrs starting at ZT2) Mouse liver were crosslinked with 1% formaldehyde for 10min, and nuclei were purified using standard sucrose cushion protocol. Nuclei were resuspended, flash frozen in liquid nitrogen and stored at -80C. Nuclei were thawed on ice, pelleted down and chromatin was digested with micrococcal nuclease. Mnase digestion was performed for 15min using 30,000 gel unit per reaction Following chromatin digestion with Mnase, H2AZ-containing nucleosome were imunoprecipitated using an anti-H2AZ antibody. Immunoprecipitated chromatin (mononucleosome fraction) was purified and used for generating illumina libraries Illumina libraries were generated using standard protocol: end repair, add A, adapter ligation, amplification, gel purification.
GEO Accession
GSM1178898