RNA-Seq of Stropharia rugosoannulata
There are two main ways to obtain mRNA: one is to use the structural features of most eukaryotic mRNAs with polyA tails, and to enrich the mRNAs with polyA tails through Oligo (dT) magnetic beads. The second is to remove ribosomal RNA from total RNA to obtain mRNA. Subsequently, the obtained mRNA is randomly interrupted with divalent cations in the NEB Fragmentation Buffer, and the library is constructed according to the NEB normal library building method or the chain-specific library building method.