Whole-genome sequening of the Caenorhabditis tropicalis strain QX2345: Nearly isogenic line carrying qqIR46 (III:10.42-10.46 Mb; NIC203 > EG6180)
DNA was extracted using the Masterpure Complete DNA and RNA Purification Kit, and WGS libraries were generated using a library prep protocol similar to the Illumina Nextera Library Prep Kit, but uses a homebrew version of the TN5 transposase carrying mutations described in the following reference: Kia, A., Gloeckner, C., Osothprarop, T. et al. Improved genome sequencing using an engineered transposase. BMC Biotechnol 17, 6 (2017).