GSM4210941: B_breve_C_2A; Bifidobacterium breve DSM 20213 = JCM 1192; RNA-Seq
acidic-phenol/guanidine-isothiocyanate protocol 1)mRNA enrichment: Oligo dT Selection or rRNA depletion;2)RNA fragment and reverse transcription:  Fragment the RNA and reverse transcription to double-strand cDNA (dscDNA) by N6 random  primer;3)End repair, add A tailing and adaptor ligation:The synthesized cDNA was subjected to end-repair  and then was 3' adenylated. Adaptors were ligated to the ends of these 3' adenylated cDNA  fragments;4)PCR amplification:The ligation products were purified and many rounds of PCR amplification  were performed to enrich the purified cDNA template using PCR primer;5)Denature and  cyclization:Denature the PCR product by heat and the single strand DNA is cyclized by splint oligo and  DNA ligase;6) Sequencing on BGISEQ-500 platform.
GEO Accession
GSM4210941