GSM3780546: Mock infected cells; Homo sapiens; RNA-Seq
Mock and infected COs were dissociated as described above with the exception washes and collection were done in 1X PBS with 1% bovine serum albumin. Cells were counted and diluted to 106/mL prior to loading onto the 10X Genomics Chromium Controller instrument to be individually partitioned into nanoliter scale droplets within an oil emulsion. RNA sequencing libraries were constructed using the Chromium Single cell 3? Reagent kit V2 (10X Genomics PN-120237) and assayed using a Bioanalyzer 2100 (Agilent) according to the respective manufacturer?s instructions.
GEO Accession
GSM3780546