Pooled sample consisting of samples: Cyst 941 S1|Cyst 902 S1|Cyst 926 S1|Cyst 919 S1|Cyst 912 S1|Cyst 920 S1|Cyst 928 S1|Cyst 913 S1|Cyst 930 S1|Cyst 938 S1|Cyst 923 S1|Cyst 916 S1|Cyst 932 S1
Primers were used to amplify DNA in 25-uL multiplex polymerase chain reactions (PCRs) as described previously 15.  For each sample, three multiplex PCRs were performed, with each multiplex PCR containing 22 to 50 primer pairs.  Reactions were purified with AMPure XP beads (Beckman Coulter) and eluted in 100 uL of Buffer EB (Qiagen, cat. no. 19086).  0.25% of the purified PCR products were then amplified in a second round of PCR, as described in Springer et al.15.  The PCR products were purified with AMPure and used for sequencing on a MiSeq or HiSeq instrument