ABI SOLiD analysis of the B. subtilis transcriptome under different growth conditions: DMSO
The Applied Biosystems SOLiDTM Total RNA-Seq kit (catalog number 4445374) was used to generate the cDNA template library. The SOLiDTM EZ Bead system was used to perform emulsion clonal bead amplification to generate bead templates for SOLiDTM platform sequencing. Samples were sequenced on either the SOLiDTM 5500xl platform or the SOLiDTM 4 platform. The samples sequenced on the SOLiDTM 5500xl platform consisted of the two biological replicates (cultures supplemented independently).