GSM4710396: scATAC-seq E14.5 somatosensory cortex rep2; Mus musculus; ATAC-seq
The somatosensory cortex of E14.5 mouse embryos was isolated and meninges removed. Single cell suspension was prepared using the papain-based neural dissociation kit (Miltenyi Biotec, Cat. N: 130-092-628) according to the manufacturer protocol with reduced dissociation times (5 min. instead of 15 min. at 37˚C). Cell number as well as the viability was assessed using the Countess™ II Automated Cell Counter (Invitrogen). Directly after tissue dissociation scATAC-seq (10x Genomics, Cat. N: PN-1000110, PN-1000086, PN-1000084) libraries were generated according to the manual instructions with a targeted recovery of 6000 cells/nuclei per sample. Before sequencing, libraries were quantified by qPCR using the NEBNext® Library Quant Kit (New England Biolabs, Cat. N: E7630S). The size distribution of the obtained libraries was assessed using Agilent 2100 Bioanalyzer.
GEO Accession
GSM4710396