Exome sequencing of human neutrophils and CD4+ T cells
Peripheral leukapheresis blood was collected from a healthy Caucasian male donor, obtained from AllCells, LLC, Emeryville, CA. Red blood cells were depleted by sedimentation using the HetaSep solution. Neutrophils were isolated by using the EasySep human Neutrophil Enrichment Kit. Mononuclear cells were isolated by using Ficoll solution and CD4+ helper T cells were isolated from the mononuclear cells using the StemSep human Naive CD4+ T Cell Enrichment Kit. DNA was extracted from the purified cells by using the FlexiGene DNA kit. Exome DNA was extracted from DNA of neutrophils and CD4+ T cells using Illumina TruSeq exome enrichment kit. Paired-end exome sequencing (2x100) was performed at 200x exome coverage per sample using an Illumina HiSeq 2000 sequencer.