Peru_hCov
First-strand cDNA was synthesized using K-8N primer and SuperScript III Reverse Transcriptase, then convert the first-strand cDNA into double-stranded cDNA using Klenow polymerase, then sequence-independent PCR amplification was conducted using primer K and Platinum Taq DNA Polymerase High Fidelity. The cDNA obtained was subjected to next-generation sequencing (NGS) using the Nextera XT kit and the MiSeq Illumina sequencer.