IsoSeq analysis of Murraya koenigii
Iso-seq libraries were prepared as per PacBio recommendations. 1st strand cDNA was synthesised from 1ug of total RNA followed by second strand synthesis. The double stranded cDNA was divided into 3 size fractions (1-3 Kb, 3-6 kb and 5-10kb). The 1-3kb and 3-6 kb fractions were pooled in equimolar concentrations into a single fraction. SMRT bell libraries were prepared using SMRTbell template prep kit 1.0 SPv3 for each of the two size fractions. DNA polymerase binding was carried out using Sequel Binding and Internal Cntrl Kit 2.1 and libraries were sequenced using Sequel Sequencing Kit 2.1 v2 on PacBio sequel platform with a movie time of 10 hrs.