DNA Pool-seq of adult female flies: F18 generation of recombination between Drosophila americana females and D. novamexicana males
A total of 157 females (F18 generation) were divided and pooled into four quartiles based on eye size (corrected for body size). The gDNA of the fourth quartile (40 flies showing the largest eye size) was extracted using phenol/chloroform. Libraries were prepared using the kit TruSeq Nano DNA Library Prep from Illumina.