single_mismatch_run3_emx1.3
Barcode sequence Illumina barcode sequence Primer sequence Target sequence Target orientation Sample  TCGGAATG CATGATCG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-1 BR1 TCGGAATG AGGATCTA GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-2 BR1 TCGGAATG GACAGTAA GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-3 BR1 TCGGAATG CCTATGCC GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-4 BR1 TCGGAATG TCGCCTTG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-5 BR1 TCGGAATG ATAGCGTC GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-6 BR1 TCGGAATG GAAGAAGT GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-7 BR1 TCGGAATG ATTCTAGG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-8 BR1 TCGGAATG CGTTACCA GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-9 BR1 TCGGAATG GTCTGATG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-10 BR1 TCGGAATG TTACGCAC GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-11 BR1 TCGGAATG TTGAATAG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-12 BR1 AACGCATT CATGATCG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-13 BR1 AACGCATT AGGATCTA GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-14 BR1 AACGCATT GACAGTAA GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-15 BR1 AACGCATT CCTATGCC GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-16 BR1 AACGCATT TCGCCTTG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-17 BR1 AACGCATT ATAGCGTC GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-18 BR1 AACGCATT GAAGAAGT GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-19 BR1 AACGCATT ATTCTAGG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-20 BR1 AACGCATT CGTTACCA GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-21 BR1 AACGCATT GTCTGATG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-22 BR1 AACGCATT TTACGCAC GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-23 BR1 AACGCATT TTGAATAG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-24 BR1 CGCGCGGT CATGATCG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-25 BR1 CGCGCGGT AGGATCTA GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-26 BR1 CGCGCGGT GACAGTAA GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-27 BR1 CGCGCGGT CCTATGCC GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-28 BR1 CGCGCGGT TCGCCTTG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-29 BR1 CGCGCGGT ATAGCGTC GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-30 BR1 CGCGCGGT GAAGAAGT GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-31 BR1 CGCGCGGT ATTCTAGG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-32 BR1 CGCGCGGT CGTTACCA GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-33 BR1 CGCGCGGT GTCTGATG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-34 BR1 CGCGCGGT TTACGCAC GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-35 BR1 CGCGCGGT TTGAATAG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-36 BR1 TCTGGCGA CATGATCG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-37 BR1 TCTGGCGA AGGATCTA GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-38 BR1 TCTGGCGA GACAGTAA GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-39 BR1 TCTGGCGA CCTATGCC GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-40 BR1 TCTGGCGA TCGCCTTG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-41 BR1 TCTGGCGA ATAGCGTC GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-42 BR1 TCTGGCGA GAAGAAGT GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-43 BR1 TCTGGCGA ATTCTAGG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-44 BR1 TCTGGCGA CGTTACCA GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-45 BR1 TCTGGCGA GTCTGATG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-46 BR1 TCTGGCGA TTACGCAC GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-47 BR1 TCTGGCGA TTGAATAG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-48 BR1 CATAGCGA CATGATCG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-49 BR1 CATAGCGA AGGATCTA GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-50 BR1 CATAGCGA GACAGTAA GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-51 BR1 CATAGCGA CCTATGCC GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-52 BR1 CATAGCGA TCGCCTTG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-53 BR1 CATAGCGA ATAGCGTC GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-54 BR1 CATAGCGA GAAGAAGT GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-55 BR1 CATAGCGA ATTCTAGG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-56 BR1 CATAGCGA CGTTACCA GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-57 BR1 CATAGCGA GTCTGATG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-no mutations BR1 CATAGCGA TTACGCAC GGAGGACAAAGTACAAACGGC  + Cas9 BR1 CATAGCGA TTGAATAG GGAGGACAAAGTACAAACGGC  + (-) BR1 CAGGAGCC CATGATCG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-1 BR2 CAGGAGCC AGGATCTA GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-2 BR2 CAGGAGCC GACAGTAA GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-3 BR2 CAGGAGCC CCTATGCC GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-4 BR2 CAGGAGCC TCGCCTTG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-5 BR2 CAGGAGCC ATAGCGTC GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-6 BR2 CAGGAGCC GAAGAAGT GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-7 BR2 CAGGAGCC ATTCTAGG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-8 BR2 CAGGAGCC CGTTACCA GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-9 BR2 CAGGAGCC GTCTGATG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-10 BR2 CAGGAGCC TTACGCAC GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-11 BR2 CAGGAGCC TTGAATAG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-12 BR2 TGTCGGAT CATGATCG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-13 BR2 TGTCGGAT AGGATCTA GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-14 BR2 TGTCGGAT GACAGTAA GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-15 BR2 TGTCGGAT CCTATGCC GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-16 BR2 TGTCGGAT TCGCCTTG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-17 BR2 TGTCGGAT ATAGCGTC GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-18 BR2 TGTCGGAT GAAGAAGT GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-19 BR2 TGTCGGAT ATTCTAGG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-20 BR2 TGTCGGAT CGTTACCA GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-21 BR2 TGTCGGAT GTCTGATG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-22 BR2 TGTCGGAT TTACGCAC GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-23 BR2 TGTCGGAT TTGAATAG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-24 BR2 ATTATGTT CATGATCG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-25 BR2 ATTATGTT AGGATCTA GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-26 BR2 ATTATGTT GACAGTAA GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-27 BR2 ATTATGTT CCTATGCC GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-28 BR2 ATTATGTT TCGCCTTG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-29 BR2 ATTATGTT ATAGCGTC GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-30 BR2 ATTATGTT GAAGAAGT GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-31 BR2 ATTATGTT ATTCTAGG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-32 BR2 ATTATGTT CGTTACCA GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-33 BR2 ATTATGTT GTCTGATG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-34 BR2 ATTATGTT TTACGCAC GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-35 BR2 ATTATGTT TTGAATAG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-36 BR2 CCTACCAT CATGATCG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-37 BR2 CCTACCAT AGGATCTA GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-38 BR2 CCTACCAT GACAGTAA GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-39 BR2 CCTACCAT CCTATGCC GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-40 BR2 CCTACCAT TCGCCTTG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-41 BR2 CCTACCAT ATAGCGTC GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-42 BR2 CCTACCAT GAAGAAGT GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-43 BR2 CCTACCAT ATTCTAGG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-44 BR2 CCTACCAT CGTTACCA GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-45 BR2 CCTACCAT GTCTGATG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-46 BR2 CCTACCAT TTACGCAC GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-47 BR2 CCTACCAT TTGAATAG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-48 BR2 TACTTAGC CATGATCG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-49 BR2 TACTTAGC AGGATCTA GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-50 BR2 TACTTAGC GACAGTAA GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-51 BR2 TACTTAGC CCTATGCC GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-52 BR2 TACTTAGC TCGCCTTG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-53 BR2 TACTTAGC ATAGCGTC GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-54 BR2 TACTTAGC GAAGAAGT GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-55 BR2 TACTTAGC ATTCTAGG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-56 BR2 TACTTAGC CGTTACCA GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-57 BR2 TACTTAGC GTCTGATG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA + EMX1.3-no mutations BR2 TACTTAGC TTACGCAC GGAGGACAAAGTACAAACGGC  + Cas9 BR2 TACTTAGC TTGAATAG GGAGGACAAAGTACAAACGGC  + (-) BR2 