Transcriptome analysis of differentially expressed genes associated with reproduction in golden mahseer (Tor putitora)
The paired end cDNA sequencing libraries from brain RNA of female golden mahseer were prepared using illumina TruSeq mRNA Library preparation kit, as per its described protocol. The amplified libraries were analyzed in Bioanalyser 2100 using high sensitivity DNA chip. Library was loaded into Illumina platform for cluster generation and sequencing.The library was sequenced using 2*150 PE chemistry to generate around 5GB data. De novo transcriptome assembly was carried out using Bridger (r2014-12-01) on default parameters.CDS was predicted from Unigenes using TransDecoder software.