GSM2616805: HeLa_G3BP1-METTL3KD-0h_RNA-seq to determine mRNA half-lives; Homo sapiens; RNA-Seq
RNA was extracted using Qiagen RNA miniprep kit. For RNA-seq experiment,  RNA was purified and ERCC spike-in was added. rRNA was depleted using RiboZero kit. Library was prepared using in-house protocols. For PAR-CLIP experiment, RNA was purified and the purified bound RNA was dissolved in 12 µl RNase-free water, of which 6 µl was subjected to small RNA library preparation with NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (E7300S, NEB).
GEO Accession
GSM2616805