Effects of patulin on Sporobolomyces sp. cells
Sporobolomyces sp. IAM 13481 cells were incubated for 3 hours in the presence of 5 µg/ml of patulin. Total RNA was extracted from frozen Sporobolomyces sp. cells with the Trizol reagent according to the manufacturer's instruction (Invitrogen, Grand Island, NY). Quality and concentration of total RNA was checked by gel electrophoresis and a spectrophotometer, respectively. Libraries construction and Illumina sequencing were performed at the DNA Core Facility of the University of Missouri - Columbia. TruSeq strand-specific libraries were prepared since this method allows a more accurate generation of a reference transcriptome. 100 bp single end reads were sequenced using an Illumina HiSeq2000 instrument.