GSM1470178: RTG3-myc_gcn4d_IP1; Saccharomyces cerevisiae; ChIP-Seq
Cells were lysed in a beadbeater and then sonicated.  The supernatant was collected and used for immunoprecipitation.  A sample was removed and labeled as the INPUT sample. Immunoprecipitations were performed using anti-c-myc resin. Samples were incubated overnight at 70°C to reverse cross-links.  Samples were RNAse treated and DNA was isolated by Phenol:Chlorofom extraction. ChIP DNA was blunt ended, had addition of “A” base to 3’ end, and had sequencing adapters ligated to the ends. The fragments were size selected to 200-600 base pairs, and underwent amplification for 15 cycles. The resulting libraries were sequenced using the Illumina HiSeq-2000 as single reads extending 42 bases.
GEO Accession
GSM1470178