GSM4710808: miRNA EXOCTRL14; Homo sapiens; miRNA-Seq
venous blood (5 ml) was collected in sodium citrate tubes. Within 1 h it was centrifuged at 1000 ×g for 15 min to separate plasma, followed by an additional centrifugation at 1600 ×g for 20 min to remove platelets Small RNA libraries were constructed from the RNA samples using NEBNext® kit (New England Biolabs, USA). Individually-barcoded libraries were mixed. Pools were size selected on Novex 10% TBE gels (Life Technologies, USA) to enrich for miRNAs fraction. Sequencing (75 nts single-end) was performed on Illumina NextSeq500 (Illumina, USA). Long RNA libraries (mRNAs and lncRNAs) were prepared with the Illumina TruSeq Stranded RNA Library Prep (Illumina, USA). Sequencing (75 nts paired-end) was performed on Illumina NextSeq500 (Illumina, USA). Demultiplexing was done as described for miRNA sequences.
GEO Accession
GSM4710808