RNA-Seq analysis of Arabidopsis thaliana 3-week seedlings (shoot) by 0uM MeJA treatment in jaz7 bio rep 1
Total RNAs were extracted from shoots or roots using the Qiagen RNeasy Plant Mini kit, and the quality and quantity of the extracted RNAs were determined by the Agilent 2100 Bioanalyzer. mRNAs were purified from 25 μg of total RNA using a MicroPoly(A) PuristTM kit. The ERCC RNA Spike-In control was added to mRNA before fragmentation for validation and normalization. Libraries were constructed using Illumina TruSeqTM RNA Sample Prep kit v2 according to the manufacturer’s instructions. Three-week-old seedlings of Col-0 wild-type and the jaz2-1 and jaz7 mutants growing under 0 and 10 μM MeJA were dissected into shoots and roots. Approximately 100 mg of fresh tissues were obtained from 10 and 100 seedlings for shoots and roots, respectively. Three biological replicates were included in each treatment, resulting in 18 samples per tissue type (3 genotypes * 2 conditions * 3 biological replicates).