Marine sediment community from Little Sippewissett Salt Marsh, Falmount, MA, United States - SSM7 Cy5- A
The V4 region of the 16S rRNA gene was amplified using universal primers (515-806) using 28 cycles of PCR. PCR products were purified then barcodes and sequencing adapters were attached with 8 cycles of a second PCR. Products were purified once more, then quantified using PicoGreen. Final purified PCR products were pooled at equimolar ratios prior to sequencingusing Illumina 2x250bp paired-end read MiSeq sequencing.