DNA-Seq of P. aeruginosa AA2
DNA was extracted with Wizard Genomic DNA Purification Kit (Promega). DNA was quantified using a Qubit 2.0 fluorometer and a standard SMRTbell library was prepared with 10Kb insertions. Sequencing was performed on a PacBio Sequel system (Pacific Biosciences) with SMRTbell Template Prep Kit 1.0-SPv3 (Pacific Biosciences). Library preparation and PacBio sequencing were performed at Novogene Co.,LTD.