GSM3780673: BPLER_DMSO_H3K27ac_ChIPSeq; Homo sapiens; ChIP-Seq
Lysates from formaldehyde cross-linked cells were clarified from sonicated nuclei and histone-DNA complexes were immunoprecipitated with antibody. Cross-links were reversed and DNA extracted for library construction. Libraries for paired-end sequencing and alignment were prepared according to NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina® according to manufacturer instructions (New England BioLabs).