Target enrichment of UCEs and exons from Sublegatus obscurior (Subleg_obsior_SB11764)
We extracted whole genomic DNA from frozen tissues using standard methods and kits (Qiagen, Valencia, CA). Toepad DNA was extracted using a similar strategy, but in an ancient DNA lab where contamination risks were minimized (detailed protocol available at https://github.com/mgharvey/tyranni). We quantified DNA extracts using a Qubit fluorometer and sent them to Rapid Genomics (Gainesville, FL) for library preparation and sequence capture following the general protocol of Faircloth et al. 2012. This protocol involves fragmenting the genomic DNA, ligating barcoded Illumina-compatible adapters, PCR amplification, probe hybridization, and enrichment of hybridized fragments.