GSM4710968: H3K27me3_Panob_3; Homo sapiens; ChIP-Seq
Lysates were clarified from sonicated samples and histone-DNA complexes were isolated with antibody. Genomic DNA was extracted and purified using Qiagen PCR Purification Kit according to standard instructions Libraries were prepared with the NEBNext® ChIP-Seq Library Prep Reagent Set for Illumina (10 ng input) following the manufacturers' instructions. A size selection step for fragments of 300 bp length was performed after library preparation. Pools of six to seven libraries were sequenced on one lane HiSeq 4000 in 50 single read mode.
GEO Accession
GSM4710968