SPF_FS5
RNA samples with RIN values greater than 8 were processed for downstream library preparation and sequencing. Briefly, 500 ng total RNA per sample extracted from zooids of Fredericella sultana, were used for library preparation with the TruSeq RNA Library Prep Kit v2 (Illumina) according to the manufacturers protocol. Library quality control was done with the D1000 ScreenTape assay (Agilent, Santa Clara, CA, USA) on the 4200 TapeStation. Sequencing of libraries (paired-end 150-bp reads) were performed on llumina NextSeq550 platform.RNA samples with RIN values greater than 8 were processed for downstream library preparation and sequencing. Briefly, 500 ng total RNA per sample extracted from zooids of Fredericella sultana, were used for library preparation with the TruSeq RNA Library Prep Kit v2 (Illumina) according to the manufacturers protocol. Library quality control was done with the D1000 ScreenTape assay (Agilent, Santa Clara, CA, USA) on the 4200 TapeStation. Sequencing of libraries (paired-end 150-bp reads) were performed on llumina NextSeq550 platform.RNA samples with RIN values greater than 8 were processed for downstream library preparation and sequencing. Briefly, 500 ng total RNA per sample extracted from zooids of Fredericella sultana, were used for library preparation with the TruSeq RNA Library Prep Kit v2 (Illumina) according to the manufacturers protocol. Library quality control was done with the D1000 ScreenTape assay (Agilent, Santa Clara, CA, USA) on the 4200 TapeStation. Sequencing of libraries (paired-end 150-bp reads) were performed on llumina NextSeq550 platform.