GSM4210295: MTECplus Drop-seq; Mus musculus; RNA-Seq
Organoids were dissociated using Accutase and Trypsin-EDTA. mRNA was captured using original Drop-seq methods DNA polymerase for pre-amplification step (1 cycle of 95°C for 3 min, 15-17 cycles of 98°C for 15 sec, 65°C for 30 sec, 68°C for 4 min and 1 cycle of 72°C for 10 min) was replaced by Terra PCR Direct Polymerase (#639271, Takara). The other processes were performed as described in the original Drop-seq protocol. single-cell RNA-seq (3' enrichment)
GEO Accession
GSM4210295