Global Transcription Profiling Reveals Two Distinct Outcomes in Central Nervous System Infections of Rabies Virus
The mice were anesthetized with ketamine-xylazine (intraperitoneally) and given intranasal doses of 10,000 focus-forming units (FFU) of either CVS-11 or HEP-FLury in 50 µL of phosphate buffered saline (PBS) and the mock-infected control mice were treated with PBS. At the indicated time points, mice were anesthetized and then perfused by intracardiac injection of PBS. The brain tissues were removed, lysed in TRizol reagent (Invitrogen, USA). Total RNAs, isolated from the mice brains (n = 3) representing the groups of CVS-11 infected mice at 10 days post infection (dpi), HEP-Flury infected mice at 10, 18, 25 dpi and mock-infected mice, were used for transcriptome analysis. mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) and processed for high-throughput sequencing on an Illumina Hiseq 2000 platform, and 100 bp single-end reads were generated according to the standard Illumina protocols.