GSM2443356: AZA non-responder PD7166_EOT; Homo sapiens; RNA-Seq
Bone marrow CD34+ cells were isolated from mononuclear cells from MDS or CMML patients. RNA was then isolated from 100,000 CD34+ cells using Qiagen Rneasy Mini kits as per manufacturer's recommendations. 30ng of RNA was amplified using NuGEN Ovation RNA-seq v2 kits and the resulting amplified ds cDNA was used for library preparation following standard Illumina protocol. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM2443356