GSM1833711: mDIPseq MCF7 hypoxia exp 3, rep B; Homo sapiens; OTHER
Nucleic acids were subsequently extracted using the Wizard Genomic DNA Purification (Promega, Leiden, The Netherlands) kit according to instructions, with all buffers supplemented with DFO (200 µM), dissolved in 80 µL PBS-DFO with RNAse A (200 units, NEB, Ipswich, MA, USA), incubated for 10 minutes at 37°C. After proteinase K addition (200 units) and incubation for 30 minutes at 56°C, DNA was purified using the QIAQuick blood and tissue kit (all buffers supplemented with DFO), eluted in 100 µL of a 10 mM Tris, 1mM EDTA solution (pH 8) and stored at -80°C until further processing. Library preparations and DNA immunoprecipitations were as described by Taiwo and colleagues (Nat Protoc 2012), using established antibodies targeting 5mC  and 5hmC. DIP-seq
GEO Accession
GSM1833711