RNA-Seq of a leaf color mutant: Haworthia cooperi var. pilfera
Total RNA was extracted using Trizol. The quality and quantity of total RNA were assessed at an absorbance ratio and 1% agarose gel electrophoresis. Replicates were mixed to supply 2 pooled samples for the sequencing analysis. After mRNA-seq libraries were generated using the VAHTS mRNA-seq v2 Library Prep Kit for Illumina (Vazyme, NR601), the library concentration was measured using Qubit RNA Assay Kit in Qubit 3.0 to preliminary quantify. Insert size was assessed using the Agilent Bioanalysis 2100 system, and after the insert size consistent with expectations, qualified insert size was accurate quantified using qPCR. The clustering of the index-coded samples was performed on a cBot Cluster Generation System (Illumia, USA). After cluster generation, the library preparations were sequenced on an Illumia Hiseq X Ten platform.