GSM3207051: bead-free DNA; synthetic construct; OTHER
Drop-Seq beads were vortexed for 1 minute, sonicated for 5 minutes, and incubated at 4°C for 12 hours. The resulting supernatent was used for downstream library construction. The bead-free single-stranded DNAs were hybridized to a primer, and then converted to double-stranded DNA molecules which were then PCR amplified, purified by Ampure XP beads and gel electrophoresis.
GEO Accession
GSM3207051