GSM4211131: KOmLN1; Mus musculus; RNA-Seq
60 LTi-like cells were sorted by FACS by 7AAD-Lin-CD45+CD90+CD127+CD4+Rorc+ into cell lysis with ribonuclease inhibitor. Then we carried out the amplification by the Smart-Seq2 method. The cDNA was sheared randomly by ultrasonic waves for Illumina library preparation protocol including DNA fragmentation, end repair, 3' ends A-tailing, adapter ligation, PCR amplification and library validation.
GEO Accession
GSM4211131