GSM4211169: Nano-45; Homo sapiens; RNA-Seq
Extracted from cultured HEK293 cells using TRIzol, followed by DNase I treatment Total RNA was enriched for circRNAs by removing rRNAs (RiboMinus Eukaryote Kit) and using RNase R. Reverse transcription was performed with ProtoScript II Reverse Transcriptase. CircDNA was precipitated and used as template for RCA using the TempliPhi 100 Amplification Kit. Debranching was performed by annealing RCA products in a thermocycler using T7 Endonuclease I, followed by isopropanol precipitation. Fragments at 3-50 kb were used for library construction. Prepared using the Oxford Nanopore Ligation Sequencing Kit (#SQK-LSK109)
GEO Accession
GSM4211169