Xylem RNA-Seq
Total RNA was extracted from the xylem of White spruce (PG29) using methodology from Kolosova et al 2004. Quality and quantity was checked using Agilent 2100 BioAnalyzer. The libraries were constructed at the British Columbia Genome Sciences Centre (GSC).  The 300bp fractions were made into libraries using RNAseq chemistry; GSC protocol SLX-IPET.  Fragments were then indexed (ACAGTG), paired end, and sequenced by Illumina HiSeq-10 with a read length of 150bp.  The goal of the experiment is to study the gene richness and composition specific to xylem tissue from white spruce.