cDNA sequencing of Paracoccus RNA generation 490, C-limited
RNA was extracted from a pellet of 2 ml C-limited chemostat culture, generation 490, as described in Smith CJ et al. (2007) with minor modifications. Extracted RNA was treated with DNase (Promega) and purified using RNeasy MinElute spin columns (Qiagen). Ribosomal RNA was depleted from 5 μg total RNA using the Ribo-ZeroTM rRNA Removal Kit (Bacteria) (epicentre). Barcoded illumina cDNA libraries were prepared from approximately 100 ng of the rRNA depleted total RNA following the TruSeq RNA Sample Preparation Kit v2 manual.