RNA-seq of Brugia malayi adult male head region
RNA was extracted from dissected tissue with Trizol and Zymo Direct-zol RNA miniprep. Total RNA was subject to ribosomal RNA reduction with the Illumina Ribo-Zero ScriptSeq Complete Gold (Blood) kit. rRNA-reduced RNA was sequenced on an Illumina HiSeq 2500 with a read length of 1x100.