GSM4251998: 1357.5 Sample 2; Callithrix jacchus; Bisulfite-Seq
genomic DNA was extracted using qiagen DNAeasy Blood and Tissue Kit RRBS uses MspI which is a methylation insensitive restriction enzyme that recognizes CCGG sites. As MspI is insensitive to methylation status, all CCGG sites were cut between the two C's resulting in small fragments with a high frequency of potential CpG methylation sites. Following MspI digestion, the fragments underwent adaptor ligation, final repair, bisulfite conversion, and PCR amplification to produce the final libraries for sequencing. The 5 pools were sequenced on 5 lanes in the HiSeq 4000 (Illumina, San Diego, CA) with paired-end reads 100nt in length RRBS Methyl-Seq System 1-16
GEO Accession
GSM4251998