Whole mtDNA sequencing of CMT2A patients andhealthy maternal relatives
The entire mtDNA of available patients and controls was amplified in three fragments using three sets of primers, as previously described (Avital et al., 2012). For each of the analyzed samples, the amplified fragments were mixed in equimolar ratios and sent for library construction and sequencing utilizing the Illumina MiSeq platform (Technion Genome Center, Israel).