De novo assembly of leaf transcriptome of the recently formed allopolyploids Tragopogon and its diploid parents: T. dubius Bio Rep 2
Total RNAs were extracted from 6-week-old leaf tissues using a modified CTAB method, and their quality and quantify were determined by the Agilent 2100 Bioanalyzer. mRNAs were purified from 100 μg of total RNA using a MicroPoly(A) PuristTM kit. The ERCC RNA Spike-In control was added to mRNA before fragmentation for validation and normalization. Libraries were constructed using Illumina TruSeqTM RNA Sample Prep kit v2 according to the manufacturer’s instructions.