16S rRNA gene sequencing of Homo sapiens: urinary microbiota of premenopausal women
The V3-V4 region of the bacterial 16S rRNA gene was amplified using the primers 341F and 802R published by Klindworth et. al (2013). The 16S amplicon was sequenced in a 300-bp paired-end run on the Illumina MiSeq as outlined in the Illumina 16S Sample Preparation Guide.