GSM2793980: NPA1-HTP; Saccharomyces cerevisiae; OTHER
Cell lysis was perfomed using Zirconium beads (5x 1minutes, with 1 minutes intervals on ice). Complex was isolated by two succesive Ips. Library was constructed by linking to digested RNA illumina compatible 5' Adaptaters and 3' linkers. Then, those modified RNA were extracted and a Reverse Transcription was performed using Superscript III followed by a final moderate PCR enrichement (16 cycles). This was then analyzed on HiSeq plateform from illumina. CRAC
GEO Accession
GSM2793980