Chanute_Paxton_1
Amplicons of a portion of the nuclear large subunit ribosomal RNA gene from DNA extractions of fine plant roots from both remnant grasslands in the United States and nearby paired disturbed sites. Amplicons were produced through PCR using forward primer LROR (Bunyard et al 1994) and barcoded reverse primer FLR2 (Trouvelot et al 1999), and were paired-end sequenced (non-overlapping) with nominally 300 bp Read 1 and 300 bp Read 2.