GSM1467744: IgG control; Homo sapiens; RIP-Seq
Cells were lysed using LB1 buffer, the nuclear fraction was then lysed with M-PER (Thermo Scientific) buffer and diluted 10-fold in dilution buffer before the immunoprecipitation. Rabbit α-m6A antibody (Synaptic Systems) and rabbit IgG control bound to protein A Dynabeads were used for the immunoprecipitations. The immunoprecipitated RNA was eluted with N6-methyladenosine (Sigma-Aldrich), ethanol precipitated and resuspended in water. RNA was barcoded using ScriptSeq V2 kit (Epicentre) and sent for sequencing.
GEO Accession
GSM1467744