Illumina HiScanSQ sequencing; FAANG Reduced Representation Bisulfate Analysis of horse tissues
Reduced Representation Bisulfite Sequencing of Equine tissues
https://www.diagenode.com/files/products/kits/Premium_RRBS_kit_manual_24rxns.pdf
assay type
methylation profiling by high throughput sequencing
sample storage
frozen, -70 freezer
sample storage processing
cryopreservation in liquid nitrogen (dead tissue)
sampling to preparation interval
10
experimental protocol
https://www.diagenode.com/files/products/kits/Premium_RRBS_kit_manual_24rxns.pdf
extraction protocol
https://www.diagenode.com/en/documents/premium-rrbs-kit-manual-24rxns
library preparation location
National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland
library preparation location longitude
19.788281
library preparation location latitude
50.084838
library preparation date
2017-01-25
sequencing location
National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland
sequencing location longitude
19.788281
sequencing location latitude
50.084838
sequencing date
2017-02-15
experiment target
DNA methylation
library selection
RRBS
bisulfite conversion protocol
https://www.diagenode.com/files/products/kits/Premium_RRBS_kit_manual_24rxns.pdf
pcr product isolation protocol
https://www.diagenode.com/files/products/kits/Premium_RRBS_kit_manual_24rxns.pdf
bisulfite conversion percent
98.14481
restriction enzyme
MspI
min fragment size selection range
184
max fragment size selection range
606