MeRIP-seq of BT-549: IP
Total RNA was isolated and purified by using RNeasy mini kit (Qiagen, Germany). The RNA was treated by chemical reagent to fragmentation. The fragmental RNA was incubated with m6A antibody for immunoprecipitation according to the manufactures instruction (MeRIP m6A Kit, Merck Millipore, USA). m6A-enriched RNA was used to generate the cDNA library in parallel with the input RNA, and then sequenced using the HiSeq 4000 system (Illumina, USA). All sequencing reads were mapped to a reference human genome sequence (hg37) assembly by TopHat (Version 2.0.6).