Multiple epigenetic mechanisms and the piRNA pathway enforce LINE1 silencing during adult spermatogenesis
Multiple epigenetic mechanisms and the piRNA pathway enforce LINE1 silencing during adult spermatogenesis
MiliDAH and Miwi2DAH mice were crossed with Oct4/GFP trangenic mice to label germ cells with eGFP. To isolate E16.5 and postnatal day 7 germ cells, single cell suspensions of testis were obtained by two step enzymatic digestion and GFP-positive FACS-sorted. Mili and Miwi2 RNPs were immunoprecipitated as described in Reuter, M. et al. (2009). Small RNA libraries were generated with the protocol described in Hafner M. et al. (2008) but using adaptors suitable for sequencing on the Ilumina platform. Small RNA libraries were generated with the protocol described in Hafner M. et al. (2008) but using adaptors suitable for sequencing on the Ilumina platform.
Experimental Factor: immunoprecipitate
Miwi
Experimental Factor: genotype
Mili_Ctl