GSM4251619: pH73_4; Homo sapiens; RNA-Seq
Cells were lyzed and mRNA extracted using Qiagen RNEasy Mini kit including on-column DNAse digestion. cDNA was generated with SMART-Seq v4 Ultra Low Imput RNA Kit for Sequencing (Takara). Multiplexed pair-end libraries 100 nt in length were obtained using Nextera XT kit (Clontech). Sequencing was performed in the same sequencing unit of NovaSeq (Illumina) (100-nt-length reads, paired end) with an average depth of 15 million reads per sample.
GEO Accession
GSM4251619