GSM1173808: RNA-Seq_hs_fc_53yr; Homo sapiens; RNA-Seq
Total RNA was purified from tissue using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions. mRNA-Seq libraries were generated from total RNA with polyA+ selection of mRNA using the TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego, CA). Strand-specific libraries were constructed using a dUTP methodology as described previously [Zhong S. et al., High-Throughput Illumina Strand-Specific RNA Sequencing Library Preparation. Cold Spring Harb. Protoc. 2011, 940-949 (2011)], while non-strand-specific libraries were constructed with the TruSeq RNA Sample prep Kit v2 as per manufacturer's instructions.
GEO Accession
GSM1173808