GSM4210010: Control Rep 2 T2; Rattus norvegicus; RNA-Seq
After supernatants were collected, cells from each time point were treated with TRIzol® and then scraped and collected into tubes. Chloroform was added to each tube and after shaking, cells were poured into pre-spun phase-lock gel tubes (5PRIME). Tubes were then spun in a cold room, the upper phase was collected, and IPA and glycogen were added to each tube followed by gentle inversion. Supernatants were again spun in a cold room and the resulting pellet was washed twice with 75% ethanol. The pellet was semi-dried and then dissolved in nuclease-free H2O. RNA samples were treated with DNA-free DNA removal kit (Ambion/Invitrogen), according to manufacturer's instructions, to remove any remaining DNA. RNA was quantified using the Qubit RNA broad range kit and sample integrity assessed using Agilent. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM4210010