GSM4710464: RNA-Seq Dev Cor/Conj 20PCW 14556; Homo sapiens; RNA-Seq
Cornea-conjunctival tissue was excised from donated eyes and dissociated to single cells using a multi tissue dissociation kit (Miltenyi Biotec). Dissociation time varied from 15 to 45 minutes depending on the age of the tissue and a gentleMACS Dissociator (Miltenyi Biotec) was used to aid dissociation of the adult tissue. For scRNA-Seq libraries were generated using the Chromium Single Cell 3′ Library & Gel Bead Kit, version 3 (10x Genomics). For scATAC-Seq libraries were generated using the Chromium Single Cell ATAC Library & Gel Bead Kit, version 1 (10x Genomics). RNA-Seq & ATAC-Seq
GEO Accession
GSM4710464