16S rRNA sequencing of Daphnia magna microbiota - sulfamethoxazole
DNA was extracted from a sample of 5 whole Daphnia magna using the spin-column protocol from Qiagen's DNEasy Blood & Tissue Kit. Extracted DNA was amplified using PCR with the 515f/806r universal primer pair for the V4 region of the 16S rRNA gene. All samples were normalized using the SequalPrep Normalization Plate Kit, and quality was checked using both the Agilent High Sensitivity DNA Kit and the KAPA Library Quantification Kit. Samples were then pooled and spiked with PhiX DNA. The pooled libraries were then sequenced using the Illumina MiSeq Reagenet Kit v2 (300-cycles).