Shotgun metagenome of Homo sapiens skin: P12-Wound
50  L of extracted DNA was fragmented by Adaptive Focused Acoustics  technology (Covaris). DNA libraries were constructed using GeneRead DNA Library I Core Kit (Qiagen) according to the manufacturer s protocol with the use of barcode adaptors instead of the GeneRead Adapter I Set. Custom index primers were used for the enrichment of DNA libraries using a limited 14-cycle PCR. Libraries were then quantified using the Agilent DNA 1000 Kit (Agilent Technologies) on an Agilent 2100 Bioanalyzer (Agilent Technologies) and normalized by pooling in equimolar concentrations. Paired-end sequencing (2 * 101 bp reads) was performed on an Illumina HiSeq 2000 Instrument. Human DNA reads that might identify the patient were removed by mapping against GRCh38 with the bwa mem mapper (v0.7.10-r789) using default settings. All unmapped reads were uploaded in fastq format.