Pooled sample consisting of samples: Cyst 557 S1|Cyst 573 S1|Cyst 581 S1|Cyst 589 S1|Cyst 510 S1|Cyst 542 S1|Cyst 574 S1|Cyst 504 S1|Cyst 544 S1|Cyst 560 S1|Cyst 584 S1|Cyst 561 S1|Cyst 593 S1|Cyst 506 S1|Cyst 578 S1|Cyst 540 S1|Cyst 564 S1|Cyst 580 S1|Cyst 588 S1
Primers were used to amplify DNA in 25-uL multiplex polymerase chain reactions (PCRs) as described previously 15.  For each sample, three multiplex PCRs were performed, with each multiplex PCR containing 22 to 50 primer pairs.  Reactions were purified with AMPure XP beads (Beckman Coulter) and eluted in 100 uL of Buffer EB (Qiagen, cat. no. 19086).  0.25% of the purified PCR products were then amplified in a second round of PCR, as described in Springer et al.15.  The PCR products were purified with AMPure and used for sequencing on a MiSeq or HiSeq instrument