multiple_mismatch_emx1.3_cas9_nickase_SSODN
Barcode sequence Illumina barcode sequence Primer sequence Target sequence Target orientation Sample Data-set Bio-rep 'AAGTAGAG' 'CATGATCG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-1 Multiple Mismatch BR1 'AAGTAGAG' 'AGGATCTA' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-2 Multiple Mismatch BR1 'AAGTAGAG' 'GACAGTAA' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-3 Multiple Mismatch BR1 'AAGTAGAG' 'CCTATGCC' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-4 Multiple Mismatch BR1 'AAGTAGAG' 'TCGCCTTG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-5 Multiple Mismatch BR1 'AAGTAGAG' 'ATAGCGTC' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-6 Multiple Mismatch BR1 'AAGTAGAG' 'GAAGAAGT' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-7 Multiple Mismatch BR1 'AAGTAGAG' 'ATTCTAGG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-8 Multiple Mismatch BR1 'AAGTAGAG' 'CGTTACCA' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-9 Multiple Mismatch BR1 'AAGTAGAG' 'GTCTGATG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-10 Multiple Mismatch BR1 'AAGTAGAG' 'TTACGCAC' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-11 Multiple Mismatch BR1 'AAGTAGAG' 'TTGAATAG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-12 Multiple Mismatch BR1 'CATGCTTA' 'CATGATCG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-13 Multiple Mismatch BR1 'CATGCTTA' 'AGGATCTA' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-14 Multiple Mismatch BR1 'CATGCTTA' 'GACAGTAA' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-15 Multiple Mismatch BR1 'CATGCTTA' 'CCTATGCC' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-16 Multiple Mismatch BR1 'CATGCTTA' 'TCGCCTTG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-17 Multiple Mismatch BR1 'CATGCTTA' 'ATAGCGTC' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-18 Multiple Mismatch BR1 'CATGCTTA' 'GAAGAAGT' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-19 Multiple Mismatch BR1 'CATGCTTA' 'ATTCTAGG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-20 Multiple Mismatch BR1 'CATGCTTA' 'CGTTACCA' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-21 Multiple Mismatch BR1 'CATGCTTA' 'GTCTGATG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-22 Multiple Mismatch BR1 'CATGCTTA' 'TTACGCAC' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-23 Multiple Mismatch BR1 'CATGCTTA' 'TTGAATAG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-24 Multiple Mismatch BR1 'GCACATCT' 'CATGATCG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-25 Multiple Mismatch BR1 'GCACATCT' 'AGGATCTA' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-26 Multiple Mismatch BR1 'GCACATCT' 'GACAGTAA' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-27 Multiple Mismatch BR1 'GCACATCT' 'CCTATGCC' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-28 Multiple Mismatch BR1 'GCACATCT' 'TCGCCTTG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-29 Multiple Mismatch BR1 'GCACATCT' 'ATAGCGTC' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-30 Multiple Mismatch BR1 'GCACATCT' 'GAAGAAGT' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-31 Multiple Mismatch BR1 'GCACATCT' 'ATTCTAGG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-32 Multiple Mismatch BR1 'GCACATCT' 'CGTTACCA' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-33 Multiple Mismatch BR1 'GCACATCT' 'GTCTGATG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-34 Multiple Mismatch BR1 'GCACATCT' 'TTACGCAC' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-35 Multiple Mismatch BR1 'GCACATCT' 'TTGAATAG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-36 Multiple Mismatch BR1 'TGCTCGAC' 'CATGATCG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-37 Multiple Mismatch BR1 'TGCTCGAC' 'AGGATCTA' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-38 Multiple Mismatch BR1 'TGCTCGAC' 'GACAGTAA' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-39 Multiple Mismatch BR1 'TGCTCGAC' 'CCTATGCC' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-40 Multiple Mismatch BR1 'TGCTCGAC' 'TCGCCTTG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-41 Multiple Mismatch BR1 'TGCTCGAC' 'ATAGCGTC' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-42 Multiple Mismatch BR1 'TGCTCGAC' 'GAAGAAGT' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-43 Multiple Mismatch BR1 'TGCTCGAC' 'ATTCTAGG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-44 Multiple Mismatch BR1 'TGCTCGAC' 'CGTTACCA' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-45 Multiple Mismatch BR1 'TGCTCGAC' 'GTCTGATG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-46 Multiple Mismatch BR1 'TGCTCGAC' 'TTACGCAC' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-47 Multiple Mismatch BR1 'TGCTCGAC' 'TTGAATAG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-48 Multiple Mismatch BR1 'AGCAATTC' 'CATGATCG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-49 Multiple Mismatch BR1 'AGCAATTC' 'AGGATCTA' 'GGAGGACAAAGTACAAACGGC' [] '+' Emx1.3-50 Multiple Mismatch BR1 'AGCAATTC' 'GACAGTAA' 'GGAGGACAAAGTACAAACGGC' [] '+' Emx1.3-51 Multiple Mismatch BR1 'AGTTGCTT' 'CATGATCG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-1 Multiple Mismatch BR2 'AGTTGCTT' 'AGGATCTA' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-2 Multiple Mismatch BR2 'AGTTGCTT' 'GACAGTAA' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-3 Multiple Mismatch BR2 'AGTTGCTT' 'CCTATGCC' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-4 Multiple Mismatch BR2 'AGTTGCTT' 'TCGCCTTG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-5 Multiple Mismatch BR2 'AGTTGCTT' 'ATAGCGTC' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-6 Multiple Mismatch BR2 'AGTTGCTT' 'GAAGAAGT' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-7 Multiple Mismatch BR2 'AGTTGCTT' 'ATTCTAGG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-8 Multiple Mismatch BR2 'AGTTGCTT' 'CGTTACCA' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-9 Multiple Mismatch BR2 'AGTTGCTT' 'GTCTGATG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-10 Multiple Mismatch BR2 'AGTTGCTT' 'TTACGCAC' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-11 Multiple Mismatch BR2 'AGTTGCTT' 'TTGAATAG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-12 Multiple Mismatch BR2 'CCAGTTAG' 'CATGATCG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-13 Multiple Mismatch BR2 'CCAGTTAG' 'AGGATCTA' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-14 Multiple Mismatch BR2 'CCAGTTAG' 'GACAGTAA' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-15 Multiple Mismatch BR2 'CCAGTTAG' 'CCTATGCC' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-16 Multiple Mismatch BR2 'CCAGTTAG' 'TCGCCTTG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-17 Multiple Mismatch BR2 'CCAGTTAG' 'ATAGCGTC' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-18 Multiple Mismatch BR2 'CCAGTTAG' 'GAAGAAGT' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-19 Multiple Mismatch BR2 'CCAGTTAG' 'ATTCTAGG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-20 Multiple Mismatch BR2 'CCAGTTAG' 'CGTTACCA' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-21 Multiple Mismatch BR2 'CCAGTTAG' 'GTCTGATG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-22 Multiple Mismatch BR2 'CCAGTTAG' 'TTACGCAC' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-23 Multiple Mismatch BR2 'CCAGTTAG' 'TTGAATAG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-24 Multiple Mismatch BR2 'TTGAGCCT' 'CATGATCG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-25 Multiple Mismatch BR2 'TTGAGCCT' 'AGGATCTA' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-26 Multiple Mismatch BR2 'TTGAGCCT' 'GACAGTAA' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-27 Multiple Mismatch BR2 'TTGAGCCT' 'CCTATGCC' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-28 Multiple Mismatch BR2 'TTGAGCCT' 'TCGCCTTG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-29 Multiple Mismatch BR2 'TTGAGCCT' 'ATAGCGTC' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-30 Multiple Mismatch BR2 'TTGAGCCT' 'GAAGAAGT' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-31 Multiple Mismatch BR2 'TTGAGCCT' 'ATTCTAGG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-32 Multiple Mismatch BR2 'TTGAGCCT' 'CGTTACCA' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-33 Multiple Mismatch BR2 'TTGAGCCT' 'GTCTGATG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-34 Multiple Mismatch BR2 'TTGAGCCT' 'TTACGCAC' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-35 Multiple Mismatch BR2 'TTGAGCCT' 'TTGAATAG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-36 Multiple Mismatch BR2 'ACACGATC' 'CATGATCG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-37 Multiple Mismatch BR2 'ACACGATC' 'AGGATCTA' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-38 Multiple Mismatch BR2 'ACACGATC' 'GACAGTAA' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-39 Multiple Mismatch BR2 'ACACGATC' 'CCTATGCC' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-40 Multiple Mismatch BR2 'ACACGATC' 'TCGCCTTG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-41 Multiple Mismatch BR2 'ACACGATC' 'ATAGCGTC' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-42 Multiple Mismatch BR2 'ACACGATC' 'GAAGAAGT' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-43 Multiple Mismatch BR2 'ACACGATC' 'ATTCTAGG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-44 Multiple Mismatch BR2 'ACACGATC' 'CGTTACCA' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-45 Multiple Mismatch BR2 'ACACGATC' 'GTCTGATG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-46 Multiple Mismatch BR2 'ACACGATC' 'TTACGCAC' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-47 Multiple Mismatch BR2 'ACACGATC' 'TTGAATAG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-48 Multiple Mismatch BR2 'GGTCCAGA' 'CATGATCG' 'GGAGGACAAAGTACAAACGGC' 'GAGTCCGAGCAGAAGAAGAA' '+' Emx1.3-49 Multiple Mismatch BR2 'GGTCCAGA' 'AGGATCTA' 'GGAGGACAAAGTACAAACGGC' [] '+' Emx1.3-50 Multiple Mismatch BR2 'GGTCCAGA' 'GACAGTAA' 'GGAGGACAAAGTACAAACGGC' [] '+' Emx1.3-51 Multiple Mismatch BR2 