h14 RNA-Seq
Sequencing library construction involved poly(dT) selection of mRNA, followed by fragmentation. The first-strand of cDNA was prepared using random hexamers. Second strand cDNA was synthesized, which was followed by end repair, addition of a single “A” base, adapter ligation, agarose gel isolation of ~200-bp cDNA and PCR amplification of the size-selected cDNA.