Human TCR repertoire sequencing: healthy donor beta chain: Sample HV13b
"TCR V(D)J rearrangements have been amplified from mRNA, using RT primers directed against the constant region and using 5'-ligation-RACE. Introduction of random nucleotide barcode sequences prior to amplification allows correction of PCR/sequencing amplification and error. First twelve bases of each read denote random barcode sequence, next twelve are indexes for multiplexing, followed by V(D)J containing reads (in reverse orientation)."