16S rRNA-Seq: Soil bacterial community
Five gram of soil was added in 100 mL diluted nutrient broth (0.8 g/L) to make a slurry, which was treated with kanamycin at an initial concentration of 256 mg/L. One milliliter of slurry was sampled after 17 hour's incubation at 30 oC, and the solid phase was extracted the genomic DNA and submitted to 16S rRNA gene Illumina sequencing.