GSM4210581: Pfalciparum_D6_hour12; Plasmodium falciparum; RNA-Seq
Samples were spun down, media aspirated, and pellet snap frozen and stored at -80C. Total RNA was extracted using a phenol-chloroform protocol as described in Leman et al 2014 (PMID: 24906319), except citrate-buffered phenol was substituted for acid phenol. The ratios of reagent to sample as found in the cited protocol were retained, with the exception of the initial thawing in TES buffer, which was changed to a 2:1 ratio of TES to sample. Libraries were prepared using Stranded Total RNA Kapa kits with rRNA Ribo-Zero Globin depletion
GEO Accession
GSM4210581