GSM3891467: 2_humgraft_rat11_2a; Homo sapiens; RNA-Seq
Grafted human ventral midbrain and grafted VM-patterned hESCs cells were dissected from rat striatum and dissociated into a single cell suspension using the papain kit single-cell suspensions were loaded onto 10x Genomics Single Cell 3′ Chips along with the reverse transcription (RT) mastermix per the manufacturer's protocol for the Chromium Single Cell 3′ Library to generate single-cell gel beads in emulsion (GEMs). cDNA amplification was done as per the guidelines from 10X Genomics. Sequencing libraries were generated with unique sample indices (SI) for each sample with the following specifications Read1 28 cycles, Read2 98 cycles, Index1 8 cycles. Libraries for samples were multiplexed and sequenced on an Illumina NextSeq 500 machine using a 150-cycle NextSeq 500/550 High Output Reagent Kit v2.