GSM4648141: AJ26: VEC C6-1 NC P8->VSMC SD-4-1-19 +DOX 0.2 5-1-19 FACS GFP LOW 18-1-19; Homo sapiens; RNA-Seq
Cells were harvested by trypsinization and resuspended in FACS buffer (1% FBS in PBS supplemented with 2mM EDTA. Cells were sorted (BD FACSAria III) for Low, medium and High intensity GFP and collected in FACS Buffer. Sorted cells were spun down and resuspended in RLT buffer (RNAeasy, Qiagen) and frozen. All samples were extracted simultaneously using the RNeasy kit and resuspended in RNAse free water. NovaSeq, PolyA selection beforehand (NEBNext® Poly(A) mRNA Magnetic Isolation Module, E7490),  NEBNext® Ultra8482 II Directional RNA Library Prep Kit for Illumina (E7760) bulk mRNAseq, paired-end, 150nt
GEO Accession
GSM4648141