SMRT of Pteroceltis tatarinowii
5 g of total RNA mixture was used to generate the first strand cDNA using the SMARTer PCR cDNA Synthesis Kit (Clontech, Palo Alto, CA, USA) and oligo (dt) primers. Double-stranded cDNA was obtained by PCR amplification, then fractionated and selected (< 4 kb and > 4 kb) using the BluePippinTM Size Selection System (Sage Science, Beverly, MA). Much more selected cDNA was produced by another PCR amplification, then constructed into two SMRT bell template libraries with the Pacific Biosciences DNA Template Prep Kit 2.0 (PacificBiosciences, MenloPark,CA).