sncRNA-Seq of Solea senegalensis: adult F1 males
Small non-coding RNAs (sncRNAs) were isolated from blood plasma samples using miRNeasy Serum/Plasma Kit (Qiagen) following manufacturer instructions, and assessment of RNA quality and quantity was performed in a 2200 TapeStation Nucleic Acid system using High Sensitivity RNA ScreenTapes (Agilent, USA). Library preparation of 11 samples were performed using NEBNext  Multiplex Small RNA Library Prep Set for Illumina (New England BioLabs Inc, USA) following manufacturer protocol, and libraries size, purity, and concentration were evaluated on a High Sensitivity D1000 ScreenTape. Normalized libraries were clustered in two pools and multiplexed sequencing (76 cycles) was done in two independent runs (6 or 5 libraries per run) by the Miseq sequencer using a MiSeq Reagent Kits v3 (Illumina, San Diego, CA), following the protocol provided by the manufacturer.