GSM4211227: input1 DNA; Ustilaginoidea virens; ChIP-Seq
1000 millions of cells was washed twice in cold PBS buffer and proteins were cross-linked to DNA by incubating the sampels with formaldehyde at a final concentration of 1%. Afterwards, samples were lysed and chromatins were obtained on ice. Chromatins were sonicated to get soluble sheared chromatin (average DNA length of 200–500 bp). One part of the soluble chromatin was saved at –20°C for input DNA, and the remainder was used for immunoprecipitation by antibody. DNA from chromatin immunoprecipitation was used to construct sequencing libraries following the protocol provided by the Illumina TruSeqChIP Sample Prep Set A and sequenced on Illumina Xten with PE 150 method.
GEO Accession
GSM4211227