Pooled sample consisting of samples: Cyst 217 S1|Cyst 223 S1|Cyst 224 S1|Cyst 226 S1|Cyst 228 S1|Cyst 238 S1|Cyst 243 S1|Cyst 248 S1|Cyst 249 S1|Cyst 250 S1|Cyst 255 S1|Cyst 260 S1|Cyst 265 S1|Cyst 267 S1|Cyst 268 S1|Cyst 276 S1|Cyst 280 S1|Cyst 281 S1|Cyst 282 S1|Cyst 285 S1|Cyst 291 S1
Primers were used to amplify DNA in 25-uL multiplex polymerase chain reactions (PCRs) as described previously 15.  For each sample, three multiplex PCRs were performed, with each multiplex PCR containing 22 to 50 primer pairs.  Reactions were purified with AMPure XP beads (Beckman Coulter) and eluted in 100 uL of Buffer EB (Qiagen, cat. no. 19086).  0.25% of the purified PCR products were then amplified in a second round of PCR, as described in Springer et al.15.  The PCR products were purified with AMPure and used for sequencing on a MiSeq or HiSeq instrument