Exome Sequencing of patients with Multiple Myeloma: Sample MMRF_2015_1_BM_CD138pos_T1_KHS5U_L08090
Whole genome libraries were prepared from high quality genomic DNA sheared to ~180bp by Covaris sonication using KAPA Biosystems Hyper Prep DNA Library Preparation kits following using hybrid selection compatible adaptors provided by Agilent Technologies.  Hybrid Capture of the whole genome libraries was conducted using the SureSelect V5+UTR kit from Agilent Technologies.  All amplification steps were perfomed using KAPA Biosystems HIFI DNA polymerase.
alignment_software
BWA-0.7.8