RNA-Seq of Halisarca dujardini cells after mechanical dissociation (Nov17)
Extracted RNA was treated by Dnase I (Ambion) according to the DNA-free Kit (Ambion) protocol. For 125bp paired-end  sequencing 1 g of RNA was depleted using Ribo-zero rRNA Removal Kit (Human / Mouse / Rat) (Illumina). The effectiveness of this procedure was monitored using the Pico mRNA protocol on the Agilent Bioanalyzer 2100 (Agilent Technologies). The cDNA library of each sample was constructed using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instruction. The cDNA library quality and fragment length distributions were verified using an Agilent 2100 DNA High Sensitivity Kit.