GSM3043164: P19_T48_HiC_replicate_2; Mus musculus; Hi-C
Cells were crosslinked and then lysed with nuclei permeabilized. DNA was then restricted and the overhangs filled in incorporating a biotinylated dCTP. Free ends were then ligated together. Crosslinks were reversed, the DNA was sheared to 200-400 bp, biotin from non-ligated ends removed and then biotinylated ligation junctions were recovered with streptavidin beads. DNA-bound beads were then used for the library preparation. NebNext Ultra library prep kit for Illumina has been used. Per PCR reaction 1/5 of DNA-bound beads has been used for amplification (5 cycles). The library was then purified using SPRI beads and captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the HiSeq2500 following the manufacturer's protocols.
GEO Accession
GSM3043164