Pooled sample consisting of samples: CystA 1057 S1|CystA 1073 S1|CystA 1010 S1|CystA 1066 S1|CystA 1051 S1|CystA 1059 S1|CystA 1052 S1|CystA 1060 S1|CystA 1092 S1|CystA 1005 S1|CystA 1037 S1|CystA 1077 S1|CystA 1085 S1|CystA 1014 S1|CystA 1038 S1|CystA 1054 S1|CystA 1062 S1|CystA 1016 S1|CystA 1056 S1
Primers were used to amplify DNA in 25-uL multiplex polymerase chain reactions (PCRs) as described previously 15.  For each sample, three multiplex PCRs were performed, with each multiplex PCR containing 22 to 50 primer pairs.  Reactions were purified with AMPure XP beads (Beckman Coulter) and eluted in 100 uL of Buffer EB (Qiagen, cat. no. 19086).  0.25% of the purified PCR products were then amplified in a second round of PCR, as described in Springer et al.15.  The PCR products were purified with AMPure and used for sequencing on a MiSeq or HiSeq instrument