GSM1832789: B106117; Bos taurus; ChIP-Seq
Lymphocytes from peripheral blood were digested with MNase (TAKARA, D2910) to generate mainly mononucleosomes with a minor fraction of dinucleosomes for histone modification mapping. Chromatin from 5×10^7 cells were used for ChIP experiment and antibodies against histone H3K27me3 (millipore, Cat. #17–622) were used, that yielded approximately 30 ng of DNA. The ChIP DNA fragments were blunt-ended and ligated to the Solexa adaptors (Paired-End DNA Sample Prep kit, Illumina, Cat. #PE-102–1001), the ChIP DNA was then amplified using the adaptor primers for 15 cycles and the fragments around 100–300 bp (mononucleosome + adaptors) were isolated from agarose gel. The purified DNA was used directly for cluster generation and sequencing analysis using the Illumina Hiseq2000 following manufacturer protocols.
GEO Accession
GSM1832789