GSM4210316: ER5_Rep2; Homo sapiens; RNA-Seq
Qiagen AllPrep Kit per the manufacturer's instructions Libraries were prepared using the KAPA RNA HyperPrep Kit with RiboErase (HMR) (KAPA Biosystems) following the manufacturer's protocol using 100 ng of total RNA. RNA was fragmented to a size of 100-200 nucleotides, adapter stock concentration used was 750 nM, and libraries were amplified for 11 cycles. Final libraries were quantified on a high sensitivity bioanalyzer chip and sequenced on the Illumina HiSeq 2500 and NextSeq 550 sequencers (Illumina, San Diego).
GEO Accession
GSM4210316