16S rRNA V4 from soft coral tissue of Gorgonia ventalina (SampleID: G12AT): with custom peptide nucleic acid clamp
The universal bacterial primers (515F-806R) for the 16S rRNA (V4 region) were used to amplify microbial DNA from coral samples. A custom peptide nucleic acid (PNA) clamp (Reigel et al. 2020) was added during PCR to mitigate host DNA contamination. Samples were sequenced on a 2x251 Illumina MiSeq run.