GSM2792453: KOII_LCMV; Mus musculus; RNA-Seq
Liver was homogenized in 750 µl Trizol reagent (Invitrogen) and vortexed after adding 150 µl chloroform and incubated for 5 min at room temperature, followed by centrifugation for 15 min at 20,000 g. 300 µl from the upper phase was transferred to a new microtube and after the addition of 70% ethanol (1:1 vol), transferred to an RNeasy column (RNeasy mini kit, Qiagen). RNA was purified following DNase I digestion on the column according to manufacturer’s instructions For library preparation, 6.1 µl of total RNA (5 ng/µl) was used for the MARS-seq  protocol (Jaitin et al. 2014 Science.343:776-9). RNA-seq libraries were sequenced using Illumina NextSeq 500 3' end-RNA-seq (DGE)
GEO Accession
GSM2792453