Human TCR repertoire sequencing: HIV+ donor on ART combined alpha + beta chains: Sample P017v2a+b
"TCR V(D)J rearrangements have been amplified from mRNA, using RT                 primers directed against the constant region and using 5'-ligation-RACE.                 Introduction of random nucleotide barcode sequences prior to amplification allows                 correction of PCR/sequencing amplification and error. First twelve bases of each                 read denote random barcode sequence, next twelve are indexes for multiplexing,                 followed by V(D)J containing reads (in reverse orientation)."