The miRNAs and their regulatory networks responsible for pollen abortion in Ogura-CMS Chinese cabbage revealed by high-throughput sequencing of miRNAs, the degradome and the transcriptome- Tyms
The flower buds of a Chinese cabbage Ogura-CMS sterile line (Tyms) and its maintainer line (231-330) were used in this study. The lines possessed isogenic chromosomes with different cytoplasmic genes. The plants were cultivated in the same experimental plots in the Henan Academy of Agricultural Sciences (Yuanyang, Henan Province, China). Buds with a length of 0-6 mm were stripped from ten different plants for RNA profiling. All fertile buds were gathered together (as were the sterile buds), snap-frozen in liquid nitrogen and kept at -80°C for further use. Total RNA was isolated according to the instructions of the TRIzol kit (Invitrogen, USA).