GSM4648520: RNAseq_RBMX_KD1_1; Homo sapiens; RNA-Seq
Total RNA was isolated from MOLM13 cells 2 days after transduction with lentivirus expressing either control scramble shRNA or shRNAs targeting RBMX/L1 (shRNA1 and shRNA2) using TRIZOL and the RNeasy RNA extraction kit (n = 4 for each group). RNA was denatured, and the first strand of cDNA was synthesized using oligo (dT)-primer-containing Illumina-compatible linker sequence. After removal of RNA, the second cDNA strand was synthesized with a random decamer containing another Illumina-compatible linker sequence. Illumina-compatible annealing sequences and external barcodes were introduced during amplification of the libraries.
GEO Accession
GSM4648520