GSM2966530: ATAC-50K-ESC_rep2; Mus musculus; ATAC-seq
Native cells were collected for 50,000 each group. Cells were lysed gently and tagmented by Tn5-MEA/B. After tagmentation, adaptor-ligated DNA was immediately extracted using QIAGEN QIAquick kit. DNA was amplified for 14 cycles with NEBNext Q5 Ultra II master mix. The desirable size range was 200-800 bp, size-selected using AMPure XP beads. The sequencing strategy was on HiSeq 4000, for paired end 150 bp.
GEO Accession
GSM2966530