Analysis of the interferon-induced changes in transcriptome of primary human hepatocytes at three time points by RNA-seq- Control mock treated samples run1
Total cellular RNA was extracted from primary hepato- cytes using TRIzol Reagent (Invitrogen) followed by DNase I treatment (Affymetrix), phenol:chloroform extraction and ethanol precipitation. The RNA pellets were dissolved with RNase free water and treated by Ribo-Zero rRNA Removal kit (Epicenter) to remove ribosomal RNAs. RNA-seq li- braries were made with Scriptseq v2 (Epicenter) or TruSeq stranded total RNA (illumina) according to the manufac- turer’s instructions.