GSM3656926: D2_rep2; Mus musculus; RNA-Seq
Retinas from both eyes were quickly removed under a dissection microscope and directly placed into 160 U/ml Ribolock® (Thermo Scientific, Walton, MA, United States) in Hank's Balanced Salt Solution (Sigma, St. Louis, MO, United States) on ice. Tissue was stored at -80°C. RNA was isolated in batches using a Qiacube and the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. The isolation included on-column DNase1 treatment to remove contaminating genomic DNA. All tissue was harvested between 10 am and noon to minimize circadian differences in gene expression. RNA integrity (RIN) was assessed on a Bioanalyzer 2100 (Agilent, Santa Clara, CA, United States). RIN-score for all samples ranged from 9.1 to 9.5. RNA was then quantified by spectrophotometry and 260/280 ratios for all samples were >2.1. Six samples of purified retinal RNA underwent library preparation with the Illumina TruSeq Stranded Total RNA kit.
GEO Accession
GSM3656926