Pooled sample consisting of samples: Cyst 203 S1|Cyst 204 S1|Cyst 207 S1|Cyst 212 S1|Cyst 215 S1|Cyst 220 S1|Cyst 233 S1|Cyst 235 S1|Cyst 240 S1|Cyst 244 S1|Cyst 245 S1|Cyst 246 S1|Cyst 247 S1|Cyst 252 S1|Cyst 254 S1|Cyst 256 S1|Cyst 257 S1|Cyst 261 S1|Cyst 262 S1|Cyst 271 S1|Cyst 273 S1|Cyst 279 S1|Cyst 283 S1|Cyst 287 S1|Cyst 290 S1
Primers were used to amplify DNA in 25-uL multiplex polymerase chain reactions (PCRs) as described previously 15.  For each sample, three multiplex PCRs were performed, with each multiplex PCR containing 22 to 50 primer pairs.  Reactions were purified with AMPure XP beads (Beckman Coulter) and eluted in 100 uL of Buffer EB (Qiagen, cat. no. 19086).  0.25% of the purified PCR products were then amplified in a second round of PCR, as described in Springer et al.15.  The PCR products were purified with AMPure and used for sequencing on a MiSeq or HiSeq instrument