1m_2mm_run2
Barcode sequence Illumina barcode sequence Primer sequence Target sequence Guide RNA Target orientation Sample Data-set CTAAGGTAAC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GACACCTCCAATGACTAGGG + Emx1.1-1 Single Mismatch TAAGGAGAAC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GCCACCTCCAATGACTAGGG + Emx1.1-2 Single Mismatch AAGAGGATTC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GGCACCTCCAATGACTAGGG + Emx1.1-3 Single Mismatch TACCAAGATC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTAACCTCCAATGACTAGGG + Emx1.1-4 Single Mismatch CAGAAGGAAC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTTACCTCCAATGACTAGGG + Emx1.1-5 Single Mismatch CTGCAAGTTC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTGACCTCCAATGACTAGGG + Emx1.1-6 Single Mismatch TTCGTGATTC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCTCCTCCAATGACTAGGG + Emx1.1-7 Single Mismatch TTCCGATAAC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCCCCTCCAATGACTAGGG + Emx1.1-8 Single Mismatch TGAGCGGAAC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCGCCTCCAATGACTAGGG + Emx1.1-9 Single Mismatch CTGACCGAAC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCAACTCCAATGACTAGGG + Emx1.1-10 Single Mismatch TCCTCGAATC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCATCTCCAATGACTAGGG + Emx1.1-11 Single Mismatch TAGGTGGTTC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCAGCTCCAATGACTAGGG + Emx1.1-12 Single Mismatch TCTAACGGAC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACATCCAATGACTAGGG + Emx1.1-13 Single Mismatch TTGGAGTGTC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACTTCCAATGACTAGGG + Emx1.1-14 Single Mismatch TCTAGAGGTC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACGTCCAATGACTAGGG + Emx1.1-15 Single Mismatch TCTGGATGAC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCACCAATGACTAGGG + Emx1.1-16 Single Mismatch TCTATTCGTC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCCCCAATGACTAGGG + Emx1.1-17 Single Mismatch AGGCAATTGC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCGCCAATGACTAGGG + Emx1.1-18 Single Mismatch TTAGTCGGAC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTACAATGACTAGGG + Emx1.1-19 Single Mismatch CAGATCCATC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTTCAATGACTAGGG + Emx1.1-20 Single Mismatch TCGCAATTAC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTGCAATGACTAGGG + Emx1.1-21 Single Mismatch TTCGAGACGC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCAAATGACTAGGG + Emx1.1-22 Single Mismatch TGCCACGAAC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCTAATGACTAGGG + Emx1.1-23 Single Mismatch AACCTCATTC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCGAATGACTAGGG + Emx1.1-24 Single Mismatch CCTGAGATAC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCTATGACTAGGG + Emx1.1-25 Single Mismatch TTACAACCTC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCCATGACTAGGG + Emx1.1-26 Single Mismatch AACCATCCGC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCGATGACTAGGG + Emx1.1-27 Single Mismatch ATCCGGAATC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCATTGACTAGGG + Emx1.1-28 Single Mismatch TCGACCACTC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCACTGACTAGGG + Emx1.1-29 Single Mismatch CGAGGTTATC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAGTGACTAGGG + Emx1.1-30 Single Mismatch TCCAAGCTGC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAAAGACTAGGG + Emx1.1-31 Single Mismatch TCTTACACAC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAACGACTAGGG + Emx1.1-32 Single Mismatch TTCTCATTGAAC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAAGGACTAGGG + Emx1.1-33 Single Mismatch TCGCATCGTTC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATAACTAGGG + Emx1.1-34 Single Mismatch TAAGCCATTGTC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATTACTAGGG + Emx1.1-35 Single Mismatch AAGGAATCGTC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATCACTAGGG + Emx1.1-36 Single Mismatch CTTGAGAATGTC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGTCTAGGG + Emx1.1-37 Single Mismatch TGGAGGACGGAC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGCCTAGGG + Emx1.1-38 Single Mismatch TAACAATCGGC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGGCTAGGG + Emx1.1-39 Single Mismatch CTGACATAATC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGAATAGGG + Emx1.1-40 Single Mismatch TTCCACTTCGC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGATTAGGG + Emx1.1-41 Single Mismatch AGCACGAATC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGAGTAGGG + Emx1.1-42 Single Mismatch CTTGACACCGC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGACAAGGG + Emx1.1-43 Single Mismatch TTGGAGGCCAGC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGACCAGGG + Emx1.1-44 Single Mismatch TGGAGCTTCCTC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGACGAGGG + Emx1.1-45 Single Mismatch TCAGTCCGAAC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGACTTGGG + Emx1.1-46 Single Mismatch TAAGGCAACCAC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGACTCGGG + Emx1.1-47 Single Mismatch TTCTAAGAGAC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGACTGGGG + Emx1.1-48 Single Mismatch TCCTAACATAAC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGACTAAGG + Emx1.1-49 Single Mismatch CGGACAATGGC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGACTATGG + Emx1.1-50 Single Mismatch TTGAGCCTATTC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGACTACGG + Emx1.1-51 Single Mismatch CCGCATGGAAC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGACTAGAG + Emx1.1-52 Single Mismatch CTGGCAATCCTC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGACTAGTG + Emx1.1-53 Single Mismatch CCGGAGAATCGC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGACTAGCG + Emx1.1-54 Single Mismatch TCCACCTCCTC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGACTAGGA + Emx1.1-55 Single Mismatch CAGCATTAATTC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGACTAGGT + Emx1.1-56 Single Mismatch TCTGGCAACGGC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGACTAGGC + Emx1.1-57 Single Mismatch TCCTAGAACAC TGCTCGAC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGACTAGGG + Emx1.1-no mutation Single Mismatch TCCTTGATGTTC TGCTCGAC CCAATGGGGAGGACATCGAT   + Emx1-1.14.17-Cas9 only Single Mismatch TCTAGCTCTTC TGCTCGAC CCAATGGGGAGGACATCGAT   + Emx1-1.14.17-no transfection Single Mismatch CTAAGGTAAC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GTCATCGATGTCCTCCCCAT - Emx1.2-1 Single Mismatch TAAGGAGAAC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GCCATCGATGTCCTCCCCAT - Emx1.2-2 Single Mismatch AAGAGGATTC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GGCATCGATGTCCTCCCCAT - Emx1.2-3 Single Mismatch TACCAAGATC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GAAATCGATGTCCTCCCCAT - Emx1.2-4 Single Mismatch CAGAAGGAAC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GATATCGATGTCCTCCCCAT - Emx1.2-5 Single Mismatch CTGCAAGTTC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GAGATCGATGTCCTCCCCAT - Emx1.2-6 Single Mismatch TTCGTGATTC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACTTCGATGTCCTCCCCAT - Emx1.2-7 Single Mismatch TTCCGATAAC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACCTCGATGTCCTCCCCAT - Emx1.2-8 Single Mismatch TGAGCGGAAC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACGTCGATGTCCTCCCCAT - Emx1.2-9 Single Mismatch CTGACCGAAC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACAACGATGTCCTCCCCAT - Emx1.2-10 Single Mismatch TCCTCGAATC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACACCGATGTCCTCCCCAT - Emx1.2-11 Single Mismatch TAGGTGGTTC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACAGCGATGTCCTCCCCAT - Emx1.2-12 Single Mismatch TCTAACGGAC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATAGATGTCCTCCCCAT - Emx1.2-13 Single Mismatch TTGGAGTGTC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATTGATGTCCTCCCCAT - Emx1.2-14 Single Mismatch TCTAGAGGTC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATGGATGTCCTCCCCAT - Emx1.2-15 Single Mismatch TCTGGATGAC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCAATGTCCTCCCCAT - Emx1.2-16 Single Mismatch TCTATTCGTC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCTATGTCCTCCCCAT - Emx1.2-17 Single Mismatch AGGCAATTGC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCCATGTCCTCCCCAT - Emx1.2-18 Single Mismatch TTAGTCGGAC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGTTGTCCTCCCCAT - Emx1.2-19 Single Mismatch CAGATCCATC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGCTGTCCTCCCCAT - Emx1.2-20 Single Mismatch TCGCAATTAC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGGTGTCCTCCCCAT - Emx1.2-21 Single Mismatch TTCGAGACGC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGAAGTCCTCCCCAT - Emx1.2-22 Single Mismatch TGCCACGAAC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGACGTCCTCCCCAT - Emx1.2-23 Single Mismatch AACCTCATTC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGAGGTCCTCCCCAT - Emx1.2-24 Single Mismatch CCTGAGATAC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATATCCTCCCCAT - Emx1.2-25 Single Mismatch TTACAACCTC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATTTCCTCCCCAT - Emx1.2-26 Single Mismatch AACCATCCGC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATCTCCTCCCCAT - Emx1.2-27 Single Mismatch ATCCGGAATC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGACCTCCCCAT - Emx1.2-28 Single Mismatch TCGACCACTC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGCCCTCCCCAT - Emx1.2-29 Single Mismatch CGAGGTTATC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGGCCTCCCCAT - Emx1.2-30 Single Mismatch TCCAAGCTGC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTACTCCCCAT - Emx1.2-31 Single Mismatch TCTTACACAC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTTCTCCCCAT - Emx1.2-32 Single Mismatch TTCTCATTGAAC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTGCTCCCCAT - Emx1.2-33 Single Mismatch TCGCATCGTTC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCATCCCCAT - Emx1.2-34 Single Mismatch TAAGCCATTGTC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCTTCCCCAT - Emx1.2-35 Single Mismatch AAGGAATCGTC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCGTCCCCAT - Emx1.2-36 Single Mismatch CTTGAGAATGTC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCACCCCAT - Emx1.2-37 Single Mismatch TGGAGGACGGAC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCCCCCCAT - Emx1.2-38 Single Mismatch TAACAATCGGC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCGCCCCAT - Emx1.2-39 Single Mismatch CTGACATAATC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTACCCAT - Emx1.2-40 Single Mismatch TTCCACTTCGC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTTCCCAT - Emx1.2-41 Single Mismatch AGCACGAATC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTGCCCAT - Emx1.2-42 Single Mismatch CTTGACACCGC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTCACCAT - Emx1.2-43 Single Mismatch TTGGAGGCCAGC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTCTCCAT - Emx1.2-44 Single Mismatch TGGAGCTTCCTC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTCGCCAT - Emx1.2-45 Single Mismatch TCAGTCCGAAC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTCCACAT - Emx1.2-46 Single Mismatch TAAGGCAACCAC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTCCTCAT - Emx1.2-47 Single Mismatch TTCTAAGAGAC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTCCGCAT - Emx1.2-48 Single Mismatch TCCTAACATAAC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTCCCAAT - Emx1.2-49 Single Mismatch CGGACAATGGC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTCCCTAT - Emx1.2-50 Single Mismatch TTGAGCCTATTC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTCCCGAT - Emx1.2-51 Single Mismatch CCGCATGGAAC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTCCCCTT - Emx1.2-52 Single Mismatch CTGGCAATCCTC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTCCCCCT - Emx1.2-53 Single Mismatch CCGGAGAATCGC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTCCCCGT - Emx1.2-54 Single Mismatch TCCACCTCCTC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTCCCCAA - Emx1.2-55 Single Mismatch CAGCATTAATTC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTCCCCAC - Emx1.2-56 Single Mismatch TCTGGCAACGGC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTCCCCAG - Emx1.2-57 Single Mismatch TCCTAGAACAC TGCTCGAC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTCCCCAT - Emx1.2-no mutation Single Mismatch TCCTTGATGTTC TGCTCGAC CGAGCAGAAGAAGAAGGGCT   - Emx1-2.16-Cas9 only Single Mismatch TCTAGCTCTTC TGCTCGAC CGAGCAGAAGAAGAAGGGCT   - Emx1-2.16-no transfection Single Mismatch CTAAGGTAAC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GAGCCACCGGTTGATGTGAT - Emx1.6-1 Single Mismatch TAAGGAGAAC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GTGCCACCGGTTGATGTGAT - Emx1.6-2 Single Mismatch AAGAGGATTC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GGGCCACCGGTTGATGTGAT - Emx1.6-3 Single Mismatch TACCAAGATC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCACCACCGGTTGATGTGAT - Emx1.6-4 Single Mismatch CAGAAGGAAC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCTCCACCGGTTGATGTGAT - Emx1.6-5 Single Mismatch CTGCAAGTTC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCCCCACCGGTTGATGTGAT - Emx1.6-6 Single Mismatch TTCGTGATTC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGACACCGGTTGATGTGAT - Emx1.6-7 Single Mismatch TTCCGATAAC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGTCACCGGTTGATGTGAT - Emx1.6-8 Single Mismatch TGAGCGGAAC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGGCACCGGTTGATGTGAT - Emx1.6-9 Single Mismatch CTGACCGAAC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCAACCGGTTGATGTGAT - Emx1.6-10 Single Mismatch TCCTCGAATC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCTACCGGTTGATGTGAT - Emx1.6-11 Single Mismatch TAGGTGGTTC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCGACCGGTTGATGTGAT - Emx1.6-12 Single Mismatch TCTAACGGAC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCTCCGGTTGATGTGAT - Emx1.6-13 Single Mismatch TTGGAGTGTC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCCCCGGTTGATGTGAT - Emx1.6-14 Single Mismatch TCTAGAGGTC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCGCCGGTTGATGTGAT - Emx1.6-15 Single Mismatch TCTGGATGAC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCAACGGTTGATGTGAT - Emx1.6-16 Single Mismatch TCTATTCGTC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCATCGGTTGATGTGAT - Emx1.6-17 Single Mismatch AGGCAATTGC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCAGCGGTTGATGTGAT - Emx1.6-18 Single Mismatch TTAGTCGGAC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACAGGTTGATGTGAT - Emx1.6-19 Single Mismatch CAGATCCATC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACTGGTTGATGTGAT - Emx1.6-20 Single Mismatch TCGCAATTAC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACGGGTTGATGTGAT - Emx1.6-21 Single Mismatch TTCGAGACGC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCAGTTGATGTGAT - Emx1.6-22 Single Mismatch TGCCACGAAC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCTGTTGATGTGAT - Emx1.6-23 Single Mismatch AACCTCATTC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCCGTTGATGTGAT - Emx1.6-24 Single Mismatch CCTGAGATAC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGATTGATGTGAT - Emx1.6-25 Single Mismatch TTACAACCTC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGTTTGATGTGAT - Emx1.6-26 Single Mismatch AACCATCCGC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGCTTGATGTGAT - Emx1.6-27 Single Mismatch ATCCGGAATC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGATGATGTGAT - Emx1.6-28 Single Mismatch TCGACCACTC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGCTGATGTGAT - Emx1.6-29 Single Mismatch CGAGGTTATC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGGTGATGTGAT - Emx1.6-30 Single Mismatch TCCAAGCTGC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTAGATGTGAT - Emx1.6-31 Single Mismatch TCTTACACAC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTCGATGTGAT - Emx1.6-32 Single Mismatch TTCTCATTGAAC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTGGATGTGAT - Emx1.6-33 Single Mismatch TCGCATCGTTC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTAATGTGAT - Emx1.6-34 Single Mismatch TAAGCCATTGTC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTTATGTGAT - Emx1.6-35 Single Mismatch AAGGAATCGTC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTCATGTGAT - Emx1.6-36 Single Mismatch CTTGAGAATGTC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGTTGTGAT - Emx1.6-37 Single Mismatch TGGAGGACGGAC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGCTGTGAT - Emx1.6-38 Single Mismatch TAACAATCGGC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGGTGTGAT - Emx1.6-39 Single Mismatch CTGACATAATC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGAAGTGAT - Emx1.6-40 Single Mismatch TTCCACTTCGC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGACGTGAT - Emx1.6-41 Single Mismatch AGCACGAATC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGAGGTGAT - Emx1.6-42 Single Mismatch CTTGACACCGC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGATATGAT - Emx1.6-43 Single Mismatch TTGGAGGCCAGC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGATTTGAT - Emx1.6-44 Single Mismatch TGGAGCTTCCTC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGATCTGAT - Emx1.6-45 Single Mismatch TCAGTCCGAAC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGATGAGAT - Emx1.6-46 Single Mismatch TAAGGCAACCAC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGATGCGAT - Emx1.6-47 Single Mismatch TTCTAAGAGAC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGATGGGAT - Emx1.6-48 Single Mismatch TCCTAACATAAC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGATGTAAT - Emx1.6-49 Single Mismatch CGGACAATGGC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGATGTTAT - Emx1.6-50 Single Mismatch TTGAGCCTATTC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGATGTCAT - Emx1.6-51 Single Mismatch CCGCATGGAAC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGATGTGTT - Emx1.6-52 Single Mismatch CTGGCAATCCTC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGATGTGCT - Emx1.6-53 Single Mismatch CCGGAGAATCGC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGATGTGGT - Emx1.6-54 Single Mismatch TCCACCTCCTC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGATGTGAA - Emx1.6-55 Single Mismatch CAGCATTAATTC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGATGTGAC - Emx1.6-56 Single Mismatch TCTGGCAACGGC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGATGTGAG - Emx1.6-57 Single Mismatch TCCTAGAACAC TGCTCGAC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGATGTGAT - Emx1.6-no mutation Single Mismatch TCCTTGATGTTC TGCTCGAC AGAAGCTGGAGGAGGAAGGG   - Emx1-6-Cas9 only Single Mismatch TCTAGCTCTTC TGCTCGAC AGAAGCTGGAGGAGGAAGGG   - Emx1-6-no transfection Single Mismatch CTAAGGTAAC AGTTGCTT CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGGCACAGATGAGAAACTC - Emx1.10-1 (no mutation) Single Mismatch TAAGGAGAAC AGTTGCTT CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGACACAGATGAGAAACTC - Emx1.10-2 Single Mismatch AAGAGGATTC AGTTGCTT CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGCCACAGATGAGAAACTC - Emx1.10-3 Single Mismatch TACCAAGATC AGTTGCTT CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGTCACAGATGAGAAACTC - Emx1.10-4 Single Mismatch CAGAAGGAAC AGTTGCTT CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGGCACAGATGAAAAACTC - Emx1.10-5 Single Mismatch CTGCAAGTTC AGTTGCTT CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGGCACAGATGACAAACTC - Emx1.10-6 Single Mismatch TTCGTGATTC AGTTGCTT CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGGCACAGATGATAAACTC - Emx1.10-7 Single Mismatch TTCCGATAAC AGTTGCTT CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGGCACAGATGAGCAACTC - Emx1.10-8 Single Mismatch TGAGCGGAAC AGTTGCTT CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGGCACAGATGAGGAACTC - Emx1.10-9 Single Mismatch CTGACCGAAC AGTTGCTT CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGGCACAGATGAGTAACTC - Emx1.10-10 Single Mismatch TCCTCGAATC AGTTGCTT CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGGCACAGATGAGAAACAC - Emx1.10-11 Single Mismatch TAGGTGGTTC AGTTGCTT CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGGCACAGATGAGAAACCC - Emx1.10-12 Single Mismatch TCTAACGGAC AGTTGCTT CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGGCACAGATGAGAAACGC - Emx1.10-13 Single Mismatch TTGGAGTGTC AGTTGCTT CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGGCACAGATGAGAAACTA - Emx1.10-14 Single Mismatch TCTAGAGGTC AGTTGCTT CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGGCACAGATGAGAAACTG - Emx1.10-15 Single Mismatch TCTGGATGAC AGTTGCTT CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGGCACAGATGAGAAACTT - Emx1.10-16 Single Mismatch CTAAGGTAAC AGTTGCTT TCATCTGTGCCCCTCCCTC GTACAAACGGCAGAAGCTGG GTACAAACGGCAGAAGCTGG + Emx1.11-1 Single Mismatch TAAGGAGAAC AGTTGCTT TCATCTGTGCCCCTCCCTC GTACAAACGGCAGAAGCTGG GTCCAAACGGCAGAAGCTGG + Emx1.11-2 Single Mismatch AAGAGGATTC AGTTGCTT TCATCTGTGCCCCTCCCTC GTACAAACGGCAGAAGCTGG GTGCAAACGGCAGAAGCTGG + Emx1.11-3 Single Mismatch TACCAAGATC AGTTGCTT TCATCTGTGCCCCTCCCTC GTACAAACGGCAGAAGCTGG GTTCAAACGGCAGAAGCTGG + Emx1.11-4 Single Mismatch CAGAAGGAAC AGTTGCTT TCATCTGTGCCCCTCCCTC GTACAAACGGCAGAAGCTGG GTACCAACGGCAGAAGCTGG + Emx1.11-5 Single Mismatch CTGCAAGTTC AGTTGCTT TCATCTGTGCCCCTCCCTC GTACAAACGGCAGAAGCTGG GTACGAACGGCAGAAGCTGG + Emx1.11-6 Single Mismatch TTCGTGATTC AGTTGCTT TCATCTGTGCCCCTCCCTC GTACAAACGGCAGAAGCTGG GTACTAACGGCAGAAGCTGG + Emx1.11-7 Single Mismatch TTCCGATAAC AGTTGCTT TCATCTGTGCCCCTCCCTC GTACAAACGGCAGAAGCTGG GTACAAACGGAAGAAGCTGG + Emx1.11-8 Single Mismatch TGAGCGGAAC AGTTGCTT TCATCTGTGCCCCTCCCTC GTACAAACGGCAGAAGCTGG GTACAAACGGGAGAAGCTGG + Emx1.11-9 Single Mismatch CTGACCGAAC AGTTGCTT TCATCTGTGCCCCTCCCTC GTACAAACGGCAGAAGCTGG GTACAAACGGTAGAAGCTGG + Emx1.11-10 Single Mismatch TCCTCGAATC AGTTGCTT TCATCTGTGCCCCTCCCTC GTACAAACGGCAGAAGCTGG GTACAAACGGCAGAAGCAGG + Emx1.11-11 Single Mismatch TAGGTGGTTC AGTTGCTT TCATCTGTGCCCCTCCCTC GTACAAACGGCAGAAGCTGG GTACAAACGGCAGAAGCCGG + Emx1.11-12 Single Mismatch TCTAACGGAC AGTTGCTT TCATCTGTGCCCCTCCCTC GTACAAACGGCAGAAGCTGG GTACAAACGGCAGAAGCGGG + Emx1.11-13 Single Mismatch TTGGAGTGTC AGTTGCTT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGAGGAGGAA + Emx1.12-1 Single Mismatch TCTAGAGGTC AGTTGCTT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GACAGAAGCTGGAGGAGGAA + Emx1.12-2 Single Mismatch TCTGGATGAC AGTTGCTT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GCCAGAAGCTGGAGGAGGAA + Emx1.12-3 Single Mismatch TCTATTCGTC AGTTGCTT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GTCAGAAGCTGGAGGAGGAA + Emx1.12-4 Single Mismatch AGGCAATTGC AGTTGCTT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAAAAGCTGGAGGAGGAA + Emx1.12-5 Single Mismatch TTAGTCGGAC AGTTGCTT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCACAAGCTGGAGGAGGAA + Emx1.12-6 Single Mismatch CAGATCCATC AGTTGCTT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCATAAGCTGGAGGAGGAA + Emx1.12-7 Single Mismatch TCGCAATTAC AGTTGCTT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAACTGGAGGAGGAA + Emx1.12-8 Single Mismatch TTCGAGACGC AGTTGCTT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAACCTGGAGGAGGAA + Emx1.12-9 Single Mismatch TGCCACGAAC AGTTGCTT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAATCTGGAGGAGGAA + Emx1.12-10 Single Mismatch AACCTCATTC AGTTGCTT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCAGGAGGAGGAA + Emx1.12-11 Single Mismatch CCTGAGATAC AGTTGCTT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCCGGAGGAGGAA + Emx1.12-12 Single Mismatch TTACAACCTC AGTTGCTT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCGGGAGGAGGAA + Emx1.12-13 Single Mismatch AACCATCCGC AGTTGCTT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTAGAGGAGGAA + Emx1.12-14 Single Mismatch ATCCGGAATC AGTTGCTT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTCGAGGAGGAA + Emx1.12-15 Single Mismatch TCGACCACTC AGTTGCTT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTTGAGGAGGAA + Emx1.12-16 Single Mismatch CGAGGTTATC AGTTGCTT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGAAGGAGGAA + Emx1.12-17 Single Mismatch TCCAAGCTGC AGTTGCTT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGCAGGAGGAA + Emx1.12-18 Single Mismatch TCTTACACAC AGTTGCTT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGTAGGAGGAA + Emx1.12-19 Single Mismatch TTCTCATTGAAC AGTTGCTT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGCGGAGGAA + Emx1.12-20 Single Mismatch TCGCATCGTTC AGTTGCTT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGGGGAGGAA + Emx1.12-21 Single Mismatch TAAGCCATTGTC AGTTGCTT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGTGGAGGAA + Emx1.12-22 Single Mismatch AAGGAATCGTC AGTTGCTT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGAAGAGGAA + Emx1.12-23 Single Mismatch CTTGAGAATGTC AGTTGCTT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGACGAGGAA + Emx1.12-24 Single Mismatch TGGAGGACGGAC AGTTGCTT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGATGAGGAA + Emx1.12-25 Single Mismatch TAACAATCGGC AGTTGCTT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGAGAAGGAA + Emx1.12-26 Single Mismatch CTGACATAATC AGTTGCTT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGAGCAGGAA + Emx1.12-27 Single Mismatch TTCCACTTCGC AGTTGCTT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGAGTAGGAA + Emx1.12-28 Single Mismatch AGCACGAATC AGTTGCTT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGAGGAAGAA + Emx1.12-29 Single Mismatch CTTGACACCGC AGTTGCTT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGAGGACGAA + Emx1.12-30 Single Mismatch TTGGAGGCCAGC AGTTGCTT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGAGGATGAA + Emx1.12-31 Single Mismatch TGGAGCTTCCTC AGTTGCTT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGAGGAGGAC + Emx1.12-32 Single Mismatch TCAGTCCGAAC AGTTGCTT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGAGGAGGAG + Emx1.12-33 Single Mismatch TAAGGCAACCAC AGTTGCTT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGAGGAGGAT + Emx1.12-34 Single Mismatch CTAAGGTAAC AGTTGCTT GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTTCTTCTTCTGCT - Emx1.13-1 Single Mismatch TAAGGAGAAC AGTTGCTT GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTTCTTCTTCAGCT - Emx1.13-2 Single Mismatch AAGAGGATTC AGTTGCTT GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTTCTTCTTCCGCT - Emx1.13-3 Single Mismatch TACCAAGATC AGTTGCTT GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTTCTTCTTCGGCT - Emx1.13-4 Single Mismatch CAGAAGGAAC AGTTGCTT GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTTCTTCTTCTGAT - Emx1.13-5 Single Mismatch CTGCAAGTTC AGTTGCTT GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTTCTTCTTCTGGT - Emx1.13-6 Single Mismatch TTCGTGATTC AGTTGCTT GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTTCTTCTTCTGTT - Emx1.13-7 Single Mismatch TCACTCGGATC AGTTGCTT CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAAACCCACGA + Emx1.14-1 Single Mismatch TTCCTGCTTCAC AGTTGCTT CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAACCCCACGA + Emx1.14-2 Single Mismatch CCTTAGAGTTC AGTTGCTT CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAAGCCCACGA + Emx1.14-3 Single Mismatch CTGAGTTCCGAC AGTTGCTT CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAATCCCACGA + Emx1.14-4 Single Mismatch TCCTGGCACATC AGTTGCTT CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAAAACCACGA + Emx1.14-5 Single Mismatch CCGCAATCATC AGTTGCTT CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAAAGCCACGA + Emx1.14-6 Single Mismatch TTCCTACCAGTC AGTTGCTT CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAAATCCACGA + Emx1.14-7 Single Mismatch TCAAGAAGTTC AGTTGCTT CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAAACACACGA + Emx1.14-8 Single Mismatch TTCAATTGGC AGTTGCTT CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAAACGCACGA + Emx1.14-9 Single Mismatch CCTACTGGTC AGTTGCTT CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAAACTCACGA + Emx1.14-10 Single Mismatch TGAGGCTCCGAC AGTTGCTT CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAAACCAACGA + Emx1.14-11 Single Mismatch CGAAGGCCACAC AGTTGCTT CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAAACCGACGA + Emx1.14-12 Single Mismatch TCTGCCTGTC AGTTGCTT CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAAACCTACGA + Emx1.14-13 Single Mismatch CGATCGGTTC AGTTGCTT CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAAACCCAAGA + Emx1.14-14 Single Mismatch TCAGGAATAC AGTTGCTT CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAAACCCAGGA + Emx1.14-15 Single Mismatch CGGAAGAACCTC AGTTGCTT CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAAACCCATGA + Emx1.14-16 Single Mismatch CGAAGCGATTC AGTTGCTT CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAAACCCACGC + Emx1.14-17 Single Mismatch CAGCCAATTCTC AGTTGCTT CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAAACCCACGG + Emx1.14-18 Single Mismatch CCTGGTTGTC AGTTGCTT CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAAACCCACGT + Emx1.14-19 Single Mismatch TCTATTCGTC AGTTGCTT CCTCAGTCTTCCCATCAGGC   - Emx1-10-Cas9 only Single Mismatch AGGCAATTGC AGTTGCTT CCTCAGTCTTCCCATCAGGC   - Emx1-10-no transfection Single Mismatch TTCTAAGAGAC AGTTGCTT TCATCTGTGCCCCTCCCTC   + Emx1-11.12-Cas9 only Single Mismatch TCCTAACATAAC AGTTGCTT TCATCTGTGCCCCTCCCTC   + Emx1-11.12-no transfection Single Mismatch TTCCGATAAC AGTTGCTT GGAGGACAAAGTACAAACGGC   + Emx1-3.13.15-Cas9 only Single Mismatch TGAGCGGAAC AGTTGCTT GGAGGACAAAGTACAAACGGC   + Emx1-3.13.15-no transfection Single Mismatch CTGACCGAAC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCAACCGG + Emx1.15-1 Single Mismatch TCCTCGAATC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCACCCATCACATCAACCGG + Emx1.15-2 Single Mismatch TAGGTGGTTC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCCCCCATCACATCAACCGG + Emx1.15-3 Single Mismatch TCTAACGGAC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCGCCCATCACATCAACCGG + Emx1.15-4 Single Mismatch TTGGAGTGTC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCCTCACATCAACCGG + Emx1.15-5 Single Mismatch TCTAGAGGTC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCGTCACATCAACCGG + Emx1.15-6 Single Mismatch TCTGGATGAC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCTTCACATCAACCGG + Emx1.15-7 Single Mismatch TCTATTCGTC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCAACACATCAACCGG + Emx1.15-8 Single Mismatch AGGCAATTGC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCACCACATCAACCGG + Emx1.15-9 Single Mismatch TTAGTCGGAC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCAGCACATCAACCGG + Emx1.15-10 Single Mismatch CAGATCCATC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCAAATCAACCGG + Emx1.15-11 Single Mismatch TCGCAATTAC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCAGATCAACCGG + Emx1.15-12 Single Mismatch TTCGAGACGC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCATATCAACCGG + Emx1.15-13 Single Mismatch TGCCACGAAC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACCTCAACCGG + Emx1.15-14 Single Mismatch AACCTCATTC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACGTCAACCGG + Emx1.15-15 Single Mismatch CCTGAGATAC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACTTCAACCGG + Emx1.15-16 Single Mismatch TTACAACCTC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACAACAACCGG + Emx1.15-17 Single Mismatch AACCATCCGC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACACCAACCGG + Emx1.15-18 Single Mismatch ATCCGGAATC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACAGCAACCGG + Emx1.15-19 Single Mismatch TCGACCACTC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATAAACCGG + Emx1.15-20 Single Mismatch CGAGGTTATC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATGAACCGG + Emx1.15-21 Single Mismatch TCCAAGCTGC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATTAACCGG + Emx1.15-22 Single Mismatch TCTTACACAC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCCACCGG + Emx1.15-23 Single Mismatch TTCTCATTGAAC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCGACCGG + Emx1.15-24 Single Mismatch TCGCATCGTTC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCTACCGG + Emx1.15-25 Single Mismatch TAAGCCATTGTC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCACCCGG + Emx1.15-26 Single Mismatch AAGGAATCGTC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCAGCCGG + Emx1.15-27 Single Mismatch CTTGAGAATGTC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCATCCGG + Emx1.15-28 Single Mismatch TGGAGGACGGAC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCAAACGG + Emx1.15-29 Single Mismatch TAACAATCGGC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCAAGCGG + Emx1.15-30 Single Mismatch CTGACATAATC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCAATCGG + Emx1.15-31 Single Mismatch TTCCACTTCGC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCAACAGG + Emx1.15-32 Single Mismatch AGCACGAATC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCAACGGG + Emx1.15-33 Single Mismatch CTTGACACCGC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCAACTGG + Emx1.15-34 Single Mismatch TTGGAGGCCAGC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCAACCAG + Emx1.15-35 Single Mismatch TGGAGCTTCCTC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCAACCCG + Emx1.15-36 Single Mismatch TCAGTCCGAAC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCAACCTG + Emx1.15-37 Single Mismatch TAAGGCAACCAC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCAACCGA + Emx1.15-38 Single Mismatch TTCTAAGAGAC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCAACCGC + Emx1.15-39 Single Mismatch TCCTAACATAAC AGTTGCTT GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCAACCGT + Emx1.15-40 Single Mismatch TCACTCGGATC AGTTGCTT CGAGCAGAAGAAGAAGGGCT GTGGCGCATTGCCACGAAGC GTGGCGCATTGCCACGAAGC + Emx1.16-1 Single Mismatch TTCCTGCTTCAC AGTTGCTT CGAGCAGAAGAAGAAGGGCT GTGGCGCATTGCCACGAAGC GTGGCGCATTGACACGAAGC + Emx1.16-2 Single Mismatch CCTTAGAGTTC AGTTGCTT CGAGCAGAAGAAGAAGGGCT GTGGCGCATTGCCACGAAGC GTGGCGCATTGGCACGAAGC + Emx1.16-3 Single Mismatch CTGAGTTCCGAC AGTTGCTT CGAGCAGAAGAAGAAGGGCT GTGGCGCATTGCCACGAAGC GTGGCGCATTGTCACGAAGC + Emx1.16-4 Single Mismatch TCCTGGCACATC AGTTGCTT CGAGCAGAAGAAGAAGGGCT GTGGCGCATTGCCACGAAGC GTGGCGCATTGCCACGACGC + Emx1.16-5 Single Mismatch CCGCAATCATC AGTTGCTT CGAGCAGAAGAAGAAGGGCT GTGGCGCATTGCCACGAAGC GTGGCGCATTGCCACGAGGC + Emx1.16-6 Single Mismatch TTCCTACCAGTC AGTTGCTT CGAGCAGAAGAAGAAGGGCT GTGGCGCATTGCCACGAAGC GTGGCGCATTGCCACGATGC + Emx1.16-7 Single Mismatch TCGAAGGCAGGC AGTTGCTT CCAATGGGGAGGACATCGAT GGCAGAGTGCTGCTTGCTGC GGCAGAGTGCTGCTTGCTGC + Emx1.17-1 Single Mismatch CCTGCCATTCGC AGTTGCTT CCAATGGGGAGGACATCGAT GGCAGAGTGCTGCTTGCTGC GGCAGCGTGCTGCTTGCTGC + Emx1.17-2 Single Mismatch TTGGCATCTC AGTTGCTT CCAATGGGGAGGACATCGAT GGCAGAGTGCTGCTTGCTGC GGCAGGGTGCTGCTTGCTGC + Emx1.17-3 Single Mismatch CTAGGACATTC AGTTGCTT CCAATGGGGAGGACATCGAT GGCAGAGTGCTGCTTGCTGC GGCAGTGTGCTGCTTGCTGC + Emx1.17-4 Single Mismatch CTTCCATAAC AGTTGCTT CCAATGGGGAGGACATCGAT GGCAGAGTGCTGCTTGCTGC GGCAGAGTGCAGCTTGCTGC + Emx1.17-5 Single Mismatch CCAGCCTCAAC AGTTGCTT CCAATGGGGAGGACATCGAT GGCAGAGTGCTGCTTGCTGC GGCAGAGTGCCGCTTGCTGC + Emx1.17-6 Single Mismatch CTTGGTTATTC AGTTGCTT CCAATGGGGAGGACATCGAT GGCAGAGTGCTGCTTGCTGC GGCAGAGTGCGGCTTGCTGC + Emx1.17-7 Single Mismatch TTGGCTGGAC AGTTGCTT CCAATGGGGAGGACATCGAT GGCAGAGTGCTGCTTGCTGC GGCAGAGTGCTGCTTACTGC + Emx1.17-8 Single Mismatch CCGAACACTTC AGTTGCTT CCAATGGGGAGGACATCGAT GGCAGAGTGCTGCTTGCTGC GGCAGAGTGCTGCTTCCTGC + Emx1.17-9 Single Mismatch TCCTGAATCTC AGTTGCTT CCAATGGGGAGGACATCGAT GGCAGAGTGCTGCTTGCTGC GGCAGAGTGCTGCTTTCTGC + Emx1.17-10 Single Mismatch CTAACCACGGC AGTTGCTT CCAATGGGGAGGACATCGAT GGCAGAGTGCTGCTTGCTGC GGCAGAGTGCTGCTTGCAGC + Emx1.17-11 Single Mismatch CGGAAGGATGC AGTTGCTT CCAATGGGGAGGACATCGAT GGCAGAGTGCTGCTTGCTGC GGCAGAGTGCTGCTTGCCGC + Emx1.17-12 Single Mismatch CTAGGAACCGC AGTTGCTT CCAATGGGGAGGACATCGAT GGCAGAGTGCTGCTTGCTGC GGCAGAGTGCTGCTTGCGGC + Emx1.17-13 Single Mismatch CTAAGGTAAC AGTTGCTT CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGGCCCAAGC + Emx1.18-1 Single Mismatch TAAGGAGAAC AGTTGCTT CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCAGCGTGGGCCCAAGC + Emx1.18-2 Single Mismatch AAGAGGATTC AGTTGCTT CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCCGCGTGGGCCCAAGC + Emx1.18-3 Single Mismatch TACCAAGATC AGTTGCTT CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCGGCGTGGGCCCAAGC + Emx1.18-4 Single Mismatch CAGAAGGAAC AGTTGCTT CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCATGGGCCCAAGC + Emx1.18-5 Single Mismatch CTGCAAGTTC AGTTGCTT CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCCTGGGCCCAAGC + Emx1.18-6 Single Mismatch TTCGTGATTC AGTTGCTT CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCTTGGGCCCAAGC + Emx1.18-7 Single Mismatch TTCCGATAAC AGTTGCTT CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGACCCAAGC + Emx1.18-8 Single Mismatch TGAGCGGAAC AGTTGCTT CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGCCCCAAGC + Emx1.18-9 Single Mismatch CTGACCGAAC AGTTGCTT CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGTCCCAAGC + Emx1.18-10 Single Mismatch TCCTCGAATC AGTTGCTT CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGGACCAAGC + Emx1.18-11 Single Mismatch TAGGTGGTTC AGTTGCTT CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGGGCCAAGC + Emx1.18-12 Single Mismatch TCTAACGGAC AGTTGCTT CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGGTCCAAGC + Emx1.18-13 Single Mismatch TTGGAGTGTC AGTTGCTT CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGGCCCCAGC + Emx1.18-14 Single Mismatch TCTAGAGGTC AGTTGCTT CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGGCCCGAGC + Emx1.18-15 Single Mismatch TCTGGATGAC AGTTGCTT CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGGCCCTAGC + Emx1.18-16 Single Mismatch TCTATTCGTC AGTTGCTT CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGGCCCAAAC + Emx1.18-17 Single Mismatch AGGCAATTGC AGTTGCTT CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGGCCCAACC + Emx1.18-18 Single Mismatch TTAGTCGGAC AGTTGCTT CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGGCCCAATC + Emx1.18-19 Single Mismatch CAGATCCATC AGTTGCTT CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCAGAGTCCAGCTT - Emx1.19-1 Single Mismatch TCGCAATTAC AGTTGCTT CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCAGAATCCAGCTT - Emx1.19-2 Single Mismatch TTCGAGACGC AGTTGCTT CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCAGACTCCAGCTT - Emx1.19-3 Single Mismatch TGCCACGAAC AGTTGCTT CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCAGATTCCAGCTT - Emx1.19-4 Single Mismatch AACCTCATTC AGTTGCTT CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCAGAGTCCCGCTT - Emx1.19-5 Single Mismatch CCTGAGATAC AGTTGCTT CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCAGAGTCCGGCTT - Emx1.19-6 Single Mismatch TTACAACCTC AGTTGCTT CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCAGAGTCCTGCTT - Emx1.19-7 Single Mismatch AACCATCCGC AGTTGCTT CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCAGAGTCCAGCAT - Emx1.19-8 Single Mismatch ATCCGGAATC AGTTGCTT CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCAGAGTCCAGCCT - Emx1.19-9 Single Mismatch TCGACCACTC AGTTGCTT CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCAGAGTCCAGCGT - Emx1.19-10 Single Mismatch CGAGGTTATC AGTTGCTT CTCCAATGACTAGGGTGGGC   + Emx1-18.19-Cas9 only Single Mismatch TCCAAGCTGC AGTTGCTT CTCCAATGACTAGGGTGGGC   + Emx1-18.19-no transfection Single Mismatch CGGACAATGGC TTGAGCCT GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTTCTTCATCTGCT - Emx1.13-8 Single Mismatch TTGAGCCTATTC TTGAGCCT GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTTCTTCCTCTGCT - Emx1.13-9 Single Mismatch CCGCATGGAAC TTGAGCCT GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTTCTTCGTCTGCT - Emx1.13-10 Single Mismatch CTGGCAATCCTC TTGAGCCT GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTTCTTCTACTGCT - Emx1.13-11 Single Mismatch CCGGAGAATCGC TTGAGCCT GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTTCTTCTCCTGCT - Emx1.13-12 Single Mismatch TCCACCTCCTC TTGAGCCT GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTTCTTCTGCTGCT - Emx1.13-13 Single Mismatch TCAAGAAGTTC TTGAGCCT CGAGCAGAAGAAGAAGGGCT GTGGCGCATTGCCACGAAGC GTGGCACATTGCCACGAAGC + Emx1.16-8 Single Mismatch TTCAATTGGC TTGAGCCT CGAGCAGAAGAAGAAGGGCT GTGGCGCATTGCCACGAAGC GTGGCCCATTGCCACGAAGC + Emx1.16-9 Single Mismatch CCTACTGGTC TTGAGCCT CGAGCAGAAGAAGAAGGGCT GTGGCGCATTGCCACGAAGC GTGGCTCATTGCCACGAAGC + Emx1.16-10 Single Mismatch TGAGGCTCCGAC TTGAGCCT CGAGCAGAAGAAGAAGGGCT GTGGCGCATTGCCACGAAGC GTGGCGCATTGCAACGAAGC + Emx1.16-11 Single Mismatch CGAAGGCCACAC TTGAGCCT CGAGCAGAAGAAGAAGGGCT GTGGCGCATTGCCACGAAGC GTGGCGCATTGCGACGAAGC + Emx1.16-12 Single Mismatch TCTGCCTGTC TTGAGCCT CGAGCAGAAGAAGAAGGGCT GTGGCGCATTGCCACGAAGC GTGGCGCATTGCTACGAAGC + Emx1.16-13 Single Mismatch TCTTACACAC TTGAGCCT CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTAGGCCCAAGC + Emx1.18-20 Single Mismatch TTCTCATTGAAC TTGAGCCT CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTCGGCCCAAGC + Emx1.18-21 Single Mismatch TCGCATCGTTC TTGAGCCT CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTTGGCCCAAGC + Emx1.18-22 Single Mismatch TAAGCCATTGTC TTGAGCCT CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGGCCCACGC + Emx1.18-23 Single Mismatch AAGGAATCGTC TTGAGCCT CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGGCCCAGGC + Emx1.18-24 Single Mismatch CTTGAGAATGTC TTGAGCCT CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGGCCCATGC + Emx1.18-25 Single Mismatch TGGAGGACGGAC TTGAGCCT CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGGCCCAAGA + Emx1.18-26 Single Mismatch TAACAATCGGC TTGAGCCT CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGGCCCAAGG + Emx1.18-27 Single Mismatch CTGACATAATC TTGAGCCT CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGGCCCAAGT + Emx1.18-28 Single Mismatch TTCCACTTCGC TTGAGCCT CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCCGAGTCCAGCTT - Emx1.19-11 Single Mismatch AGCACGAATC TTGAGCCT CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCGGAGTCCAGCTT - Emx1.19-12 Single Mismatch CTTGACACCGC TTGAGCCT CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCTGAGTCCAGCTT - Emx1.19-13 Single Mismatch TTGGAGGCCAGC TTGAGCCT CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCAGAGACCAGCTT - Emx1.19-14 Single Mismatch TGGAGCTTCCTC TTGAGCCT CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCAGAGCCCAGCTT - Emx1.19-15 Single Mismatch TCAGTCCGAAC TTGAGCCT CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCAGAGGCCAGCTT - Emx1.19-16 Single Mismatch TAAGGCAACCAC TTGAGCCT CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCAGAGTCCAACTT - Emx1.19-17 Single Mismatch TTCTAAGAGAC TTGAGCCT CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCAGAGTCCACCTT - Emx1.19-18 Single Mismatch TCCTAACATAAC TTGAGCCT CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCAGAGTCCATCTT - Emx1.19-19 Single Mismatch CTAAGGTAAC TTGAGCCT CTGCTTGCTGCTGGCCAG GGCCTCCCCAAAGCCTGGCC GGCCTCCCCAAAGCCTGGCC - Emx1.20-1 Single Mismatch TAAGGAGAAC TTGAGCCT CTGCTTGCTGCTGGCCAG GGCCTCCCCAAAGCCTGGCC GGCCTCCCCAAAGCCTGGAC - Emx1.20-2 Single Mismatch AAGAGGATTC TTGAGCCT CTGCTTGCTGCTGGCCAG GGCCTCCCCAAAGCCTGGCC GGCCTCCCCAAAGCCTGGGC - Emx1.20-3 Single Mismatch TACCAAGATC TTGAGCCT CTGCTTGCTGCTGGCCAG GGCCTCCCCAAAGCCTGGCC GGCCTCCCCAAAGCCTGGTC - Emx1.20-4 Single Mismatch CAGAAGGAAC TTGAGCCT CTGCTTGCTGCTGGCCAG GGCCTCCCCAAAGCCTGGCC GGCCTCCCCAAAGCCAGGCC - Emx1.20-5 Single Mismatch CTGCAAGTTC TTGAGCCT CTGCTTGCTGCTGGCCAG GGCCTCCCCAAAGCCTGGCC GGCCTCCCCAAAGCCGGGCC - Emx1.20-6 Single Mismatch TTCGTGATTC TTGAGCCT CTGCTTGCTGCTGGCCAG GGCCTCCCCAAAGCCTGGCC GGCCTCCCCAAAGCCCGGCC - Emx1.20-7 Single Mismatch TTCCGATAAC TTGAGCCT CTGCTTGCTGCTGGCCAG GGCCTCCCCAAAGCCTGGCC GGCCTCCCCAACGCCTGGCC - Emx1.20-8 Single Mismatch TGAGCGGAAC TTGAGCCT CTGCTTGCTGCTGGCCAG GGCCTCCCCAAAGCCTGGCC GGCCTCCCCAAGGCCTGGCC - Emx1.20-9 Single Mismatch CTGACCGAAC TTGAGCCT CTGCTTGCTGCTGGCCAG GGCCTCCCCAAAGCCTGGCC GGCCTCCCCAATGCCTGGCC - Emx1.20-10 Single Mismatch TCCTCGAATC TTGAGCCT CTGCTTGCTGCTGGCCAG GGCCTCCCCAAAGCCTGGCC GGCCTCCCCAAAGCCAGGCC - Emx1.20-11 Single Mismatch TAGGTGGTTC TTGAGCCT CTGCTTGCTGCTGGCCAG GGCCTCCCCAAAGCCTGGCC GGCCTCCCCAAAGCCCGGCC - Emx1.20-12 Single Mismatch TCTAACGGAC TTGAGCCT CTGCTTGCTGCTGGCCAG GGCCTCCCCAAAGCCTGGCC GGCCTCCCCAAAGCCGGGCC - Emx1.20-13 Single Mismatch TTGGAGTGTC TTGAGCCT CTGCTTGCTGCTGGCCAG GGCCTCCCCAAAGCCTGGCC GGCCTCCCCAAAGCCTGGAC - Emx1.20-14 Single Mismatch TCTAGAGGTC TTGAGCCT CTGCTTGCTGCTGGCCAG GGCCTCCCCAAAGCCTGGCC GGCCTCCCCAAAGCCTGGGC - Emx1.20-15 Single Mismatch TCTGGATGAC TTGAGCCT CTGCTTGCTGCTGGCCAG GGCCTCCCCAAAGCCTGGCC GGCCTCCCCAAAGCCTGGTC - Emx1.20-16 Single Mismatch TCTATTCGTC TTGAGCCT CTGCTTGCTGCTGGCCAG   - Emx1-20-Cas9 only Single Mismatch AGGCAATTGC TTGAGCCT CTGCTTGCTGCTGGCCAG   - Emx1-20-no transfection Single Mismatch CAGCATTAATTC TTGAGCCT GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA GAGACCGAGCAGAAGAAGAA + Emx1.3-1 Single Mismatch TCTGGCAACGGC TTGAGCCT GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA GAGCCCGAGCAGAAGAAGAA + Emx1.3-2 Single Mismatch TCCTAGAACAC TTGAGCCT GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA GAGGCCGAGCAGAAGAAGAA + Emx1.3-3 Single Mismatch TCCTTGATGTTC TTGAGCCT GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA GAGTCCGAGAAGAAGAAGAA + Emx1.3-4 Single Mismatch TCTAGCTCTTC TTGAGCCT GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA GAGTCCGAGGAGAAGAAGAA + Emx1.3-5 Single Mismatch TCACTCGGATC TTGAGCCT GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA GAGTCCGAGTAGAAGAAGAA + Emx1.3-6 Single Mismatch TTCCTGCTTCAC TTGAGCCT GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA GAGTCCGAGCCGAAGAAGAA + Emx1.3-7 Single Mismatch CCTTAGAGTTC TTGAGCCT GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA GAGTCCGAGCGGAAGAAGAA + Emx1.3-8 Single Mismatch CTGAGTTCCGAC TTGAGCCT GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA GAGTCCGAGCTGAAGAAGAA + Emx1.3-9 Single Mismatch TTAGTCGGAC TTGAGCCT CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGGCACAGCTGAGAAACTC - Emx1.10-17 Single Mismatch CAGATCCATC TTGAGCCT CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGGCACAGGTGAGAAACTC - Emx1.10-18 Single Mismatch TCGCAATTAC TTGAGCCT CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGGCACAGTTGAGAAACTC - Emx1.10-19 Single Mismatch CGGACAATGGC TTGAGCCT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGATGGAGGAGGAA + Emx1.12-35 Single Mismatch TTGAGCCTATTC TTGAGCCT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGGTGGAGGAGGAA + Emx1.12-36 Single Mismatch CCGCATGGAAC TTGAGCCT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGTTGGAGGAGGAA + Emx1.12-37 Single Mismatch CTGGCAATCCTC TTGAGCCT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCAGGAGGAGGAA + Emx1.12-38 Single Mismatch CCGGAGAATCGC TTGAGCCT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCCGGAGGAGGAA + Emx1.12-39 Single Mismatch TCCACCTCCTC TTGAGCCT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCGGGAGGAGGAA + Emx1.12-40 Single Mismatch CAGCATTAATTC TTGAGCCT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGAGAAGGAA + Emx1.12-41 Single Mismatch TCTGGCAACGGC TTGAGCCT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGAGCAGGAA + Emx1.12-42 Single Mismatch TCCTAGAACAC TTGAGCCT TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGAGTAGGAA + Emx1.12-43 Single Mismatch TCCTGGCACATC TTGAGCCT GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTACTTCTTCTGCT - Emx1.13-14 Single Mismatch CCGCAATCATC TTGAGCCT GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTCCTTCTTCTGCT - Emx1.13-15 Single Mismatch TTCCTACCAGTC TTGAGCCT GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTGCTTCTTCTGCT - Emx1.13-16 Single Mismatch TCAAGAAGTTC TTGAGCCT GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTTATTCTTCTGCT - Emx1.13-17 Single Mismatch TTCAATTGGC TTGAGCCT GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTTGTTCTTCTGCT - Emx1.13-18 Single Mismatch CCTACTGGTC TTGAGCCT GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTTTTTCTTCTGCT - Emx1.13-19 Single Mismatch CGGACAATGGC TTGAGCCT CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCCGAGTCCAGCTT - Emx1.19-20 Single Mismatch TTGAGCCTATTC TTGAGCCT CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCGGAGTCCAGCTT - Emx1.19-21 Single Mismatch CCGCATGGAAC TTGAGCCT CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCTGAGTCCAGCTT - Emx1.19-22 Single Mismatch TCCTGGCACATC TTGAGCCT GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA GAGTCCGAGCAGAAGAAGAA + Emx1.3-no mutation Single Mismatch CTAAGGTAAC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GACACCTCCAATGACTAGGG + Emx1.1-1 Single Mismatch TAAGGAGAAC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GCCACCTCCAATGACTAGGG + Emx1.1-2 Single Mismatch AAGAGGATTC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GGCACCTCCAATGACTAGGG + Emx1.1-3 Single Mismatch TACCAAGATC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTAACCTCCAATGACTAGGG + Emx1.1-4 Single Mismatch CAGAAGGAAC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTTACCTCCAATGACTAGGG + Emx1.1-5 Single Mismatch CTGCAAGTTC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTGACCTCCAATGACTAGGG + Emx1.1-6 Single Mismatch TTCGTGATTC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCTCCTCCAATGACTAGGG + Emx1.1-7 Single Mismatch TTCCGATAAC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCCCCTCCAATGACTAGGG + Emx1.1-8 Single Mismatch TGAGCGGAAC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCGCCTCCAATGACTAGGG + Emx1.1-9 Single Mismatch CTGACCGAAC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCAACTCCAATGACTAGGG + Emx1.1-10 Single Mismatch TCCTCGAATC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCATCTCCAATGACTAGGG + Emx1.1-11 Single Mismatch TAGGTGGTTC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCAGCTCCAATGACTAGGG + Emx1.1-12 Single Mismatch TCTAACGGAC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACATCCAATGACTAGGG + Emx1.1-13 Single Mismatch TTGGAGTGTC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACTTCCAATGACTAGGG + Emx1.1-14 Single Mismatch TCTAGAGGTC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACGTCCAATGACTAGGG + Emx1.1-15 Single Mismatch TCTGGATGAC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCACCAATGACTAGGG + Emx1.1-16 Single Mismatch TCTATTCGTC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCCCCAATGACTAGGG + Emx1.1-17 Single Mismatch AGGCAATTGC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCGCCAATGACTAGGG + Emx1.1-18 Single Mismatch TTAGTCGGAC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTACAATGACTAGGG + Emx1.1-19 Single Mismatch CAGATCCATC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTTCAATGACTAGGG + Emx1.1-20 Single Mismatch TCGCAATTAC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTGCAATGACTAGGG + Emx1.1-21 Single Mismatch TTCGAGACGC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCAAATGACTAGGG + Emx1.1-22 Single Mismatch TGCCACGAAC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCTAATGACTAGGG + Emx1.1-23 Single Mismatch AACCTCATTC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCGAATGACTAGGG + Emx1.1-24 Single Mismatch CCTGAGATAC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCTATGACTAGGG + Emx1.1-25 Single Mismatch TTACAACCTC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCCATGACTAGGG + Emx1.1-26 Single Mismatch AACCATCCGC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCGATGACTAGGG + Emx1.1-27 Single Mismatch ATCCGGAATC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCATTGACTAGGG + Emx1.1-28 Single Mismatch TCGACCACTC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCACTGACTAGGG + Emx1.1-29 Single Mismatch CGAGGTTATC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAGTGACTAGGG + Emx1.1-30 Single Mismatch TCCAAGCTGC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAAAGACTAGGG + Emx1.1-31 Single Mismatch TCTTACACAC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAACGACTAGGG + Emx1.1-32 Single Mismatch TTCTCATTGAAC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAAGGACTAGGG + Emx1.1-33 Single Mismatch TCGCATCGTTC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATAACTAGGG + Emx1.1-34 Single Mismatch TAAGCCATTGTC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATTACTAGGG + Emx1.1-35 Single Mismatch AAGGAATCGTC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATCACTAGGG + Emx1.1-36 Single Mismatch CTTGAGAATGTC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGTCTAGGG + Emx1.1-37 Single Mismatch TGGAGGACGGAC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGCCTAGGG + Emx1.1-38 Single Mismatch TAACAATCGGC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGGCTAGGG + Emx1.1-39 Single Mismatch CTGACATAATC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGAATAGGG + Emx1.1-40 Single Mismatch TTCCACTTCGC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGATTAGGG + Emx1.1-41 Single Mismatch AGCACGAATC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGAGTAGGG + Emx1.1-42 Single Mismatch CTTGACACCGC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGACAAGGG + Emx1.1-43 Single Mismatch TTGGAGGCCAGC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGACCAGGG + Emx1.1-44 Single Mismatch TGGAGCTTCCTC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGACGAGGG + Emx1.1-45 Single Mismatch TCAGTCCGAAC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGACTTGGG + Emx1.1-46 Single Mismatch TAAGGCAACCAC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGACTCGGG + Emx1.1-47 Single Mismatch TTCTAAGAGAC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGACTGGGG + Emx1.1-48 Single Mismatch TCCTAACATAAC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGACTAAGG + Emx1.1-49 Single Mismatch CGGACAATGGC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGACTATGG + Emx1.1-50 Single Mismatch TTGAGCCTATTC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGACTACGG + Emx1.1-51 Single Mismatch CCGCATGGAAC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGACTAGAG + Emx1.1-52 Single Mismatch CTGGCAATCCTC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGACTAGTG + Emx1.1-53 Single Mismatch CCGGAGAATCGC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGACTAGCG + Emx1.1-54 Single Mismatch TCCACCTCCTC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGACTAGGA + Emx1.1-55 Single Mismatch CAGCATTAATTC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGACTAGGT + Emx1.1-56 Single Mismatch TCTGGCAACGGC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGACTAGGC + Emx1.1-57 Single Mismatch TCCTAGAACAC AGCAATTC CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG GTCACCTCCAATGACTAGGG + Emx1.1-no mutation Single Mismatch TCCTTGATGTTC AGCAATTC CCAATGGGGAGGACATCGAT   + Emx1-1.14.17-Cas9 only Single Mismatch TCTAGCTCTTC AGCAATTC CCAATGGGGAGGACATCGAT   + Emx1-1.14.17-no transfection Single Mismatch CTAAGGTAAC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GTCATCGATGTCCTCCCCAT - Emx1.2-1 Single Mismatch TAAGGAGAAC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GCCATCGATGTCCTCCCCAT - Emx1.2-2 Single Mismatch AAGAGGATTC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GGCATCGATGTCCTCCCCAT - Emx1.2-3 Single Mismatch TACCAAGATC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GAAATCGATGTCCTCCCCAT - Emx1.2-4 Single Mismatch CAGAAGGAAC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GATATCGATGTCCTCCCCAT - Emx1.2-5 Single Mismatch CTGCAAGTTC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GAGATCGATGTCCTCCCCAT - Emx1.2-6 Single Mismatch TTCGTGATTC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACTTCGATGTCCTCCCCAT - Emx1.2-7 Single Mismatch TTCCGATAAC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACCTCGATGTCCTCCCCAT - Emx1.2-8 Single Mismatch TGAGCGGAAC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACGTCGATGTCCTCCCCAT - Emx1.2-9 Single Mismatch CTGACCGAAC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACAACGATGTCCTCCCCAT - Emx1.2-10 Single Mismatch TCCTCGAATC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACACCGATGTCCTCCCCAT - Emx1.2-11 Single Mismatch TAGGTGGTTC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACAGCGATGTCCTCCCCAT - Emx1.2-12 Single Mismatch TCTAACGGAC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATAGATGTCCTCCCCAT - Emx1.2-13 Single Mismatch TTGGAGTGTC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATTGATGTCCTCCCCAT - Emx1.2-14 Single Mismatch TCTAGAGGTC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATGGATGTCCTCCCCAT - Emx1.2-15 Single Mismatch TCTGGATGAC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCAATGTCCTCCCCAT - Emx1.2-16 Single Mismatch TCTATTCGTC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCTATGTCCTCCCCAT - Emx1.2-17 Single Mismatch AGGCAATTGC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCCATGTCCTCCCCAT - Emx1.2-18 Single Mismatch TTAGTCGGAC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGTTGTCCTCCCCAT - Emx1.2-19 Single Mismatch CAGATCCATC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGCTGTCCTCCCCAT - Emx1.2-20 Single Mismatch TCGCAATTAC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGGTGTCCTCCCCAT - Emx1.2-21 Single Mismatch TTCGAGACGC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGAAGTCCTCCCCAT - Emx1.2-22 Single Mismatch TGCCACGAAC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGACGTCCTCCCCAT - Emx1.2-23 Single Mismatch AACCTCATTC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGAGGTCCTCCCCAT - Emx1.2-24 Single Mismatch CCTGAGATAC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATATCCTCCCCAT - Emx1.2-25 Single Mismatch TTACAACCTC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATTTCCTCCCCAT - Emx1.2-26 Single Mismatch AACCATCCGC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATCTCCTCCCCAT - Emx1.2-27 Single Mismatch ATCCGGAATC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGACCTCCCCAT - Emx1.2-28 Single Mismatch TCGACCACTC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGCCCTCCCCAT - Emx1.2-29 Single Mismatch CGAGGTTATC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGGCCTCCCCAT - Emx1.2-30 Single Mismatch TCCAAGCTGC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTACTCCCCAT - Emx1.2-31 Single Mismatch TCTTACACAC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTTCTCCCCAT - Emx1.2-32 Single Mismatch TTCTCATTGAAC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTGCTCCCCAT - Emx1.2-33 Single Mismatch TCGCATCGTTC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCATCCCCAT - Emx1.2-34 Single Mismatch TAAGCCATTGTC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCTTCCCCAT - Emx1.2-35 Single Mismatch AAGGAATCGTC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCGTCCCCAT - Emx1.2-36 Single Mismatch CTTGAGAATGTC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCACCCCAT - Emx1.2-37 Single Mismatch TGGAGGACGGAC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCCCCCCAT - Emx1.2-38 Single Mismatch TAACAATCGGC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCGCCCCAT - Emx1.2-39 Single Mismatch CTGACATAATC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTACCCAT - Emx1.2-40 Single Mismatch TTCCACTTCGC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTTCCCAT - Emx1.2-41 Single Mismatch AGCACGAATC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTGCCCAT - Emx1.2-42 Single Mismatch CTTGACACCGC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTCACCAT - Emx1.2-43 Single Mismatch TTGGAGGCCAGC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTCTCCAT - Emx1.2-44 Single Mismatch TGGAGCTTCCTC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTCGCCAT - Emx1.2-45 Single Mismatch TCAGTCCGAAC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTCCACAT - Emx1.2-46 Single Mismatch TAAGGCAACCAC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTCCTCAT - Emx1.2-47 Single Mismatch TTCTAAGAGAC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTCCGCAT - Emx1.2-48 Single Mismatch TCCTAACATAAC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTCCCAAT - Emx1.2-49 Single Mismatch CGGACAATGGC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTCCCTAT - Emx1.2-50 Single Mismatch TTGAGCCTATTC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTCCCGAT - Emx1.2-51 Single Mismatch CCGCATGGAAC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTCCCCTT - Emx1.2-52 Single Mismatch CTGGCAATCCTC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTCCCCCT - Emx1.2-53 Single Mismatch CCGGAGAATCGC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTCCCCGT - Emx1.2-54 Single Mismatch TCCACCTCCTC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTCCCCAA - Emx1.2-55 Single Mismatch CAGCATTAATTC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTCCCCAC - Emx1.2-56 Single Mismatch TCTGGCAACGGC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTCCCCAG - Emx1.2-57 Single Mismatch TCCTAGAACAC AGCAATTC CGAGCAGAAGAAGAAGGGCT GACATCGATGTCCTCCCCAT GACATCGATGTCCTCCCCAT - Emx1.2-no mutation Single Mismatch TCCTTGATGTTC AGCAATTC CGAGCAGAAGAAGAAGGGCT   - Emx1-2.16-Cas9 only Single Mismatch TCTAGCTCTTC AGCAATTC CGAGCAGAAGAAGAAGGGCT   - Emx1-2.16-no transfection Single Mismatch CTAAGGTAAC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GAGCCACCGGTTGATGTGAT - Emx1.6-1 Single Mismatch TAAGGAGAAC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GTGCCACCGGTTGATGTGAT - Emx1.6-2 Single Mismatch AAGAGGATTC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GGGCCACCGGTTGATGTGAT - Emx1.6-3 Single Mismatch TACCAAGATC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCACCACCGGTTGATGTGAT - Emx1.6-4 Single Mismatch CAGAAGGAAC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCTCCACCGGTTGATGTGAT - Emx1.6-5 Single Mismatch CTGCAAGTTC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCCCCACCGGTTGATGTGAT - Emx1.6-6 Single Mismatch TTCGTGATTC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGACACCGGTTGATGTGAT - Emx1.6-7 Single Mismatch TTCCGATAAC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGTCACCGGTTGATGTGAT - Emx1.6-8 Single Mismatch TGAGCGGAAC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGGCACCGGTTGATGTGAT - Emx1.6-9 Single Mismatch CTGACCGAAC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCAACCGGTTGATGTGAT - Emx1.6-10 Single Mismatch TCCTCGAATC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCTACCGGTTGATGTGAT - Emx1.6-11 Single Mismatch TAGGTGGTTC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCGACCGGTTGATGTGAT - Emx1.6-12 Single Mismatch TCTAACGGAC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCTCCGGTTGATGTGAT - Emx1.6-13 Single Mismatch TTGGAGTGTC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCCCCGGTTGATGTGAT - Emx1.6-14 Single Mismatch TCTAGAGGTC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCGCCGGTTGATGTGAT - Emx1.6-15 Single Mismatch TCTGGATGAC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCAACGGTTGATGTGAT - Emx1.6-16 Single Mismatch TCTATTCGTC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCATCGGTTGATGTGAT - Emx1.6-17 Single Mismatch AGGCAATTGC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCAGCGGTTGATGTGAT - Emx1.6-18 Single Mismatch TTAGTCGGAC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACAGGTTGATGTGAT - Emx1.6-19 Single Mismatch CAGATCCATC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACTGGTTGATGTGAT - Emx1.6-20 Single Mismatch TCGCAATTAC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACGGGTTGATGTGAT - Emx1.6-21 Single Mismatch TTCGAGACGC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCAGTTGATGTGAT - Emx1.6-22 Single Mismatch TGCCACGAAC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCTGTTGATGTGAT - Emx1.6-23 Single Mismatch AACCTCATTC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCCGTTGATGTGAT - Emx1.6-24 Single Mismatch CCTGAGATAC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGATTGATGTGAT - Emx1.6-25 Single Mismatch TTACAACCTC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGTTTGATGTGAT - Emx1.6-26 Single Mismatch AACCATCCGC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGCTTGATGTGAT - Emx1.6-27 Single Mismatch ATCCGGAATC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGATGATGTGAT - Emx1.6-28 Single Mismatch TCGACCACTC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGCTGATGTGAT - Emx1.6-29 Single Mismatch CGAGGTTATC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGGTGATGTGAT - Emx1.6-30 Single Mismatch TCCAAGCTGC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTAGATGTGAT - Emx1.6-31 Single Mismatch TCTTACACAC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTCGATGTGAT - Emx1.6-32 Single Mismatch TTCTCATTGAAC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTGGATGTGAT - Emx1.6-33 Single Mismatch TCGCATCGTTC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTAATGTGAT - Emx1.6-34 Single Mismatch TAAGCCATTGTC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTTATGTGAT - Emx1.6-35 Single Mismatch AAGGAATCGTC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTCATGTGAT - Emx1.6-36 Single Mismatch CTTGAGAATGTC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGTTGTGAT - Emx1.6-37 Single Mismatch TGGAGGACGGAC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGCTGTGAT - Emx1.6-38 Single Mismatch TAACAATCGGC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGGTGTGAT - Emx1.6-39 Single Mismatch CTGACATAATC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGAAGTGAT - Emx1.6-40 Single Mismatch TTCCACTTCGC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGACGTGAT - Emx1.6-41 Single Mismatch AGCACGAATC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGAGGTGAT - Emx1.6-42 Single Mismatch CTTGACACCGC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGATATGAT - Emx1.6-43 Single Mismatch TTGGAGGCCAGC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGATTTGAT - Emx1.6-44 Single Mismatch TGGAGCTTCCTC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGATCTGAT - Emx1.6-45 Single Mismatch TCAGTCCGAAC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGATGAGAT - Emx1.6-46 Single Mismatch TAAGGCAACCAC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGATGCGAT - Emx1.6-47 Single Mismatch TTCTAAGAGAC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGATGGGAT - Emx1.6-48 Single Mismatch TCCTAACATAAC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGATGTAAT - Emx1.6-49 Single Mismatch CGGACAATGGC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGATGTTAT - Emx1.6-50 Single Mismatch TTGAGCCTATTC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGATGTCAT - Emx1.6-51 Single Mismatch CCGCATGGAAC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGATGTGTT - Emx1.6-52 Single Mismatch CTGGCAATCCTC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGATGTGCT - Emx1.6-53 Single Mismatch CCGGAGAATCGC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGATGTGGT - Emx1.6-54 Single Mismatch TCCACCTCCTC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGATGTGAA - Emx1.6-55 Single Mismatch CAGCATTAATTC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGATGTGAC - Emx1.6-56 Single Mismatch TCTGGCAACGGC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGATGTGAG - Emx1.6-57 Single Mismatch TCCTAGAACAC AGCAATTC AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT GCGCCACCGGTTGATGTGAT - Emx1.6-no mutation Single Mismatch TCCTTGATGTTC AGCAATTC AGAAGCTGGAGGAGGAAGGG   - Emx1-6-Cas9 only Single Mismatch TCTAGCTCTTC AGCAATTC AGAAGCTGGAGGAGGAAGGG   - Emx1-6-no transfection Single Mismatch CTAAGGTAAC AACTTGAC CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGGCACAGATGAGAAACTC - Emx1.10-1 (no mutation) Single Mismatch TAAGGAGAAC AACTTGAC CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGACACAGATGAGAAACTC - Emx1.10-2 Single Mismatch AAGAGGATTC AACTTGAC CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGCCACAGATGAGAAACTC - Emx1.10-3 Single Mismatch TACCAAGATC AACTTGAC CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGTCACAGATGAGAAACTC - Emx1.10-4 Single Mismatch CAGAAGGAAC AACTTGAC CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGGCACAGATGAAAAACTC - Emx1.10-5 Single Mismatch CTGCAAGTTC AACTTGAC CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGGCACAGATGACAAACTC - Emx1.10-6 Single Mismatch TTCGTGATTC AACTTGAC CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGGCACAGATGATAAACTC - Emx1.10-7 Single Mismatch TTCCGATAAC AACTTGAC CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGGCACAGATGAGCAACTC - Emx1.10-8 Single Mismatch TGAGCGGAAC AACTTGAC CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGGCACAGATGAGGAACTC - Emx1.10-9 Single Mismatch CTGACCGAAC AACTTGAC CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGGCACAGATGAGTAACTC - Emx1.10-10 Single Mismatch TCCTCGAATC AACTTGAC CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGGCACAGATGAGAAACAC - Emx1.10-11 Single Mismatch TAGGTGGTTC AACTTGAC CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGGCACAGATGAGAAACCC - Emx1.10-12 Single Mismatch TCTAACGGAC AACTTGAC CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGGCACAGATGAGAAACGC - Emx1.10-13 Single Mismatch TTGGAGTGTC AACTTGAC CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGGCACAGATGAGAAACTA - Emx1.10-14 Single Mismatch TCTAGAGGTC AACTTGAC CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGGCACAGATGAGAAACTG - Emx1.10-15 Single Mismatch TCTGGATGAC AACTTGAC CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGGCACAGATGAGAAACTT - Emx1.10-16 Single Mismatch CTAAGGTAAC AACTTGAC TCATCTGTGCCCCTCCCTC GTACAAACGGCAGAAGCTGG GTACAAACGGCAGAAGCTGG + Emx1.11-1 Single Mismatch TAAGGAGAAC AACTTGAC TCATCTGTGCCCCTCCCTC GTACAAACGGCAGAAGCTGG GTCCAAACGGCAGAAGCTGG + Emx1.11-2 Single Mismatch AAGAGGATTC AACTTGAC TCATCTGTGCCCCTCCCTC GTACAAACGGCAGAAGCTGG GTGCAAACGGCAGAAGCTGG + Emx1.11-3 Single Mismatch TACCAAGATC AACTTGAC TCATCTGTGCCCCTCCCTC GTACAAACGGCAGAAGCTGG GTTCAAACGGCAGAAGCTGG + Emx1.11-4 Single Mismatch CAGAAGGAAC AACTTGAC TCATCTGTGCCCCTCCCTC GTACAAACGGCAGAAGCTGG GTACCAACGGCAGAAGCTGG + Emx1.11-5 Single Mismatch CTGCAAGTTC AACTTGAC TCATCTGTGCCCCTCCCTC GTACAAACGGCAGAAGCTGG GTACGAACGGCAGAAGCTGG + Emx1.11-6 Single Mismatch TTCGTGATTC AACTTGAC TCATCTGTGCCCCTCCCTC GTACAAACGGCAGAAGCTGG GTACTAACGGCAGAAGCTGG + Emx1.11-7 Single Mismatch TTCCGATAAC AACTTGAC TCATCTGTGCCCCTCCCTC GTACAAACGGCAGAAGCTGG GTACAAACGGAAGAAGCTGG + Emx1.11-8 Single Mismatch TGAGCGGAAC AACTTGAC TCATCTGTGCCCCTCCCTC GTACAAACGGCAGAAGCTGG GTACAAACGGGAGAAGCTGG + Emx1.11-9 Single Mismatch CTGACCGAAC AACTTGAC TCATCTGTGCCCCTCCCTC GTACAAACGGCAGAAGCTGG GTACAAACGGTAGAAGCTGG + Emx1.11-10 Single Mismatch TCCTCGAATC AACTTGAC TCATCTGTGCCCCTCCCTC GTACAAACGGCAGAAGCTGG GTACAAACGGCAGAAGCAGG + Emx1.11-11 Single Mismatch TAGGTGGTTC AACTTGAC TCATCTGTGCCCCTCCCTC GTACAAACGGCAGAAGCTGG GTACAAACGGCAGAAGCCGG + Emx1.11-12 Single Mismatch TCTAACGGAC AACTTGAC TCATCTGTGCCCCTCCCTC GTACAAACGGCAGAAGCTGG GTACAAACGGCAGAAGCGGG + Emx1.11-13 Single Mismatch TTGGAGTGTC AACTTGAC TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGAGGAGGAA + Emx1.12-1 Single Mismatch TCTAGAGGTC AACTTGAC TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GACAGAAGCTGGAGGAGGAA + Emx1.12-2 Single Mismatch TCTGGATGAC AACTTGAC TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GCCAGAAGCTGGAGGAGGAA + Emx1.12-3 Single Mismatch TCTATTCGTC AACTTGAC TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GTCAGAAGCTGGAGGAGGAA + Emx1.12-4 Single Mismatch AGGCAATTGC AACTTGAC TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAAAAGCTGGAGGAGGAA + Emx1.12-5 Single Mismatch TTAGTCGGAC AACTTGAC TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCACAAGCTGGAGGAGGAA + Emx1.12-6 Single Mismatch CAGATCCATC AACTTGAC TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCATAAGCTGGAGGAGGAA + Emx1.12-7 Single Mismatch TCGCAATTAC AACTTGAC TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAACTGGAGGAGGAA + Emx1.12-8 Single Mismatch TTCGAGACGC AACTTGAC TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAACCTGGAGGAGGAA + Emx1.12-9 Single Mismatch TGCCACGAAC AACTTGAC TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAATCTGGAGGAGGAA + Emx1.12-10 Single Mismatch AACCTCATTC AACTTGAC TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCAGGAGGAGGAA + Emx1.12-11 Single Mismatch CCTGAGATAC AACTTGAC TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCCGGAGGAGGAA + Emx1.12-12 Single Mismatch TTACAACCTC AACTTGAC TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCGGGAGGAGGAA + Emx1.12-13 Single Mismatch AACCATCCGC AACTTGAC TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTAGAGGAGGAA + Emx1.12-14 Single Mismatch ATCCGGAATC AACTTGAC TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTCGAGGAGGAA + Emx1.12-15 Single Mismatch TCGACCACTC AACTTGAC TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTTGAGGAGGAA + Emx1.12-16 Single Mismatch CGAGGTTATC AACTTGAC TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGAAGGAGGAA + Emx1.12-17 Single Mismatch TCCAAGCTGC AACTTGAC TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGCAGGAGGAA + Emx1.12-18 Single Mismatch TCTTACACAC AACTTGAC TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGTAGGAGGAA + Emx1.12-19 Single Mismatch TTCTCATTGAAC AACTTGAC TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGCGGAGGAA + Emx1.12-20 Single Mismatch TCGCATCGTTC AACTTGAC TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGGGGAGGAA + Emx1.12-21 Single Mismatch TAAGCCATTGTC AACTTGAC TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGTGGAGGAA + Emx1.12-22 Single Mismatch AAGGAATCGTC AACTTGAC TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGAAGAGGAA + Emx1.12-23 Single Mismatch CTTGAGAATGTC AACTTGAC TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGACGAGGAA + Emx1.12-24 Single Mismatch TGGAGGACGGAC AACTTGAC TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGATGAGGAA + Emx1.12-25 Single Mismatch TAACAATCGGC AACTTGAC TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGAGAAGGAA + Emx1.12-26 Single Mismatch CTGACATAATC AACTTGAC TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGAGCAGGAA + Emx1.12-27 Single Mismatch TTCCACTTCGC AACTTGAC TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGAGTAGGAA + Emx1.12-28 Single Mismatch AGCACGAATC AACTTGAC TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGAGGAAGAA + Emx1.12-29 Single Mismatch CTTGACACCGC AACTTGAC TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGAGGACGAA + Emx1.12-30 Single Mismatch TTGGAGGCCAGC AACTTGAC TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGAGGATGAA + Emx1.12-31 Single Mismatch TGGAGCTTCCTC AACTTGAC TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGAGGAGGAC + Emx1.12-32 Single Mismatch TCAGTCCGAAC AACTTGAC TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGAGGAGGAG + Emx1.12-33 Single Mismatch TAAGGCAACCAC AACTTGAC TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGAGGAGGAT + Emx1.12-34 Single Mismatch CTAAGGTAAC AACTTGAC GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTTCTTCTTCTGCT - Emx1.13-1 Single Mismatch TAAGGAGAAC AACTTGAC GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTTCTTCTTCAGCT - Emx1.13-2 Single Mismatch AAGAGGATTC AACTTGAC GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTTCTTCTTCCGCT - Emx1.13-3 Single Mismatch TACCAAGATC AACTTGAC GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTTCTTCTTCGGCT - Emx1.13-4 Single Mismatch CAGAAGGAAC AACTTGAC GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTTCTTCTTCTGAT - Emx1.13-5 Single Mismatch CTGCAAGTTC AACTTGAC GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTTCTTCTTCTGGT - Emx1.13-6 Single Mismatch TTCGTGATTC AACTTGAC GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTTCTTCTTCTGTT - Emx1.13-7 Single Mismatch TCACTCGGATC AACTTGAC CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAAACCCACGA + Emx1.14-1 Single Mismatch TTCCTGCTTCAC AACTTGAC CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAACCCCACGA + Emx1.14-2 Single Mismatch CCTTAGAGTTC AACTTGAC CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAAGCCCACGA + Emx1.14-3 Single Mismatch CTGAGTTCCGAC AACTTGAC CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAATCCCACGA + Emx1.14-4 Single Mismatch TCCTGGCACATC AACTTGAC CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAAAACCACGA + Emx1.14-5 Single Mismatch CCGCAATCATC AACTTGAC CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAAAGCCACGA + Emx1.14-6 Single Mismatch TTCCTACCAGTC AACTTGAC CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAAATCCACGA + Emx1.14-7 Single Mismatch TCAAGAAGTTC AACTTGAC CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAAACACACGA + Emx1.14-8 Single Mismatch TTCAATTGGC AACTTGAC CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAAACGCACGA + Emx1.14-9 Single Mismatch CCTACTGGTC AACTTGAC CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAAACTCACGA + Emx1.14-10 Single Mismatch TGAGGCTCCGAC AACTTGAC CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAAACCAACGA + Emx1.14-11 Single Mismatch CGAAGGCCACAC AACTTGAC CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAAACCGACGA + Emx1.14-12 Single Mismatch TCTGCCTGTC AACTTGAC CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAAACCTACGA + Emx1.14-13 Single Mismatch CGATCGGTTC AACTTGAC CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAAACCCAAGA + Emx1.14-14 Single Mismatch TCAGGAATAC AACTTGAC CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAAACCCAGGA + Emx1.14-15 Single Mismatch CGGAAGAACCTC AACTTGAC CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAAACCCATGA + Emx1.14-16 Single Mismatch CGAAGCGATTC AACTTGAC CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAAACCCACGC + Emx1.14-17 Single Mismatch CAGCCAATTCTC AACTTGAC CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAAACCCACGG + Emx1.14-18 Single Mismatch CCTGGTTGTC AACTTGAC CCAATGGGGAGGACATCGAT GGGCAACCACAAACCCACGA GGGCAACCACAAACCCACGT + Emx1.14-19 Single Mismatch TCTATTCGTC AACTTGAC CCTCAGTCTTCCCATCAGGC   - Emx1-10-Cas9 only Single Mismatch AGGCAATTGC AACTTGAC CCTCAGTCTTCCCATCAGGC   - Emx1-10-no transfection Single Mismatch TTCTAAGAGAC AACTTGAC TCATCTGTGCCCCTCCCTC   + Emx1-11.12-Cas9 only Single Mismatch TCCTAACATAAC AACTTGAC TCATCTGTGCCCCTCCCTC   + Emx1-11.12-no transfection Single Mismatch TTCCGATAAC AACTTGAC GGAGGACAAAGTACAAACGGC   + Emx1-3.13.15-Cas9 only Single Mismatch TGAGCGGAAC AACTTGAC GGAGGACAAAGTACAAACGGC   + Emx1-3.13.15-no transfection Single Mismatch CTGACCGAAC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCAACCGG + Emx1.15-1 Single Mismatch TCCTCGAATC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCACCCATCACATCAACCGG + Emx1.15-2 Single Mismatch TAGGTGGTTC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCCCCCATCACATCAACCGG + Emx1.15-3 Single Mismatch TCTAACGGAC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCGCCCATCACATCAACCGG + Emx1.15-4 Single Mismatch TTGGAGTGTC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCCTCACATCAACCGG + Emx1.15-5 Single Mismatch TCTAGAGGTC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCGTCACATCAACCGG + Emx1.15-6 Single Mismatch TCTGGATGAC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCTTCACATCAACCGG + Emx1.15-7 Single Mismatch TCTATTCGTC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCAACACATCAACCGG + Emx1.15-8 Single Mismatch AGGCAATTGC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCACCACATCAACCGG + Emx1.15-9 Single Mismatch TTAGTCGGAC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCAGCACATCAACCGG + Emx1.15-10 Single Mismatch CAGATCCATC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCAAATCAACCGG + Emx1.15-11 Single Mismatch TCGCAATTAC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCAGATCAACCGG + Emx1.15-12 Single Mismatch TTCGAGACGC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCATATCAACCGG + Emx1.15-13 Single Mismatch TGCCACGAAC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACCTCAACCGG + Emx1.15-14 Single Mismatch AACCTCATTC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACGTCAACCGG + Emx1.15-15 Single Mismatch CCTGAGATAC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACTTCAACCGG + Emx1.15-16 Single Mismatch TTACAACCTC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACAACAACCGG + Emx1.15-17 Single Mismatch AACCATCCGC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACACCAACCGG + Emx1.15-18 Single Mismatch ATCCGGAATC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACAGCAACCGG + Emx1.15-19 Single Mismatch TCGACCACTC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATAAACCGG + Emx1.15-20 Single Mismatch CGAGGTTATC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATGAACCGG + Emx1.15-21 Single Mismatch TCCAAGCTGC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATTAACCGG + Emx1.15-22 Single Mismatch TCTTACACAC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCCACCGG + Emx1.15-23 Single Mismatch TTCTCATTGAAC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCGACCGG + Emx1.15-24 Single Mismatch TCGCATCGTTC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCTACCGG + Emx1.15-25 Single Mismatch TAAGCCATTGTC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCACCCGG + Emx1.15-26 Single Mismatch AAGGAATCGTC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCAGCCGG + Emx1.15-27 Single Mismatch CTTGAGAATGTC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCATCCGG + Emx1.15-28 Single Mismatch TGGAGGACGGAC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCAAACGG + Emx1.15-29 Single Mismatch TAACAATCGGC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCAAGCGG + Emx1.15-30 Single Mismatch CTGACATAATC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCAATCGG + Emx1.15-31 Single Mismatch TTCCACTTCGC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCAACAGG + Emx1.15-32 Single Mismatch AGCACGAATC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCAACGGG + Emx1.15-33 Single Mismatch CTTGACACCGC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCAACTGG + Emx1.15-34 Single Mismatch TTGGAGGCCAGC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCAACCAG + Emx1.15-35 Single Mismatch TGGAGCTTCCTC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCAACCCG + Emx1.15-36 Single Mismatch TCAGTCCGAAC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCAACCTG + Emx1.15-37 Single Mismatch TAAGGCAACCAC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCAACCGA + Emx1.15-38 Single Mismatch TTCTAAGAGAC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCAACCGC + Emx1.15-39 Single Mismatch TCCTAACATAAC CCAGTTAG GGAGGACAAAGTACAAACGGC GCTCCCATCACATCAACCGG GCTCCCATCACATCAACCGT + Emx1.15-40 Single Mismatch TCACTCGGATC CCAGTTAG CGAGCAGAAGAAGAAGGGCT GTGGCGCATTGCCACGAAGC GTGGCGCATTGCCACGAAGC + Emx1.16-1 Single Mismatch TTCCTGCTTCAC CCAGTTAG CGAGCAGAAGAAGAAGGGCT GTGGCGCATTGCCACGAAGC GTGGCGCATTGACACGAAGC + Emx1.16-2 Single Mismatch CCTTAGAGTTC CCAGTTAG CGAGCAGAAGAAGAAGGGCT GTGGCGCATTGCCACGAAGC GTGGCGCATTGGCACGAAGC + Emx1.16-3 Single Mismatch CTGAGTTCCGAC CCAGTTAG CGAGCAGAAGAAGAAGGGCT GTGGCGCATTGCCACGAAGC GTGGCGCATTGTCACGAAGC + Emx1.16-4 Single Mismatch TCCTGGCACATC CCAGTTAG CGAGCAGAAGAAGAAGGGCT GTGGCGCATTGCCACGAAGC GTGGCGCATTGCCACGACGC + Emx1.16-5 Single Mismatch CCGCAATCATC CCAGTTAG CGAGCAGAAGAAGAAGGGCT GTGGCGCATTGCCACGAAGC GTGGCGCATTGCCACGAGGC + Emx1.16-6 Single Mismatch TTCCTACCAGTC CCAGTTAG CGAGCAGAAGAAGAAGGGCT GTGGCGCATTGCCACGAAGC GTGGCGCATTGCCACGATGC + Emx1.16-7 Single Mismatch TCGAAGGCAGGC CCAGTTAG CCAATGGGGAGGACATCGAT GGCAGAGTGCTGCTTGCTGC GGCAGAGTGCTGCTTGCTGC + Emx1.17-1 Single Mismatch CCTGCCATTCGC CCAGTTAG CCAATGGGGAGGACATCGAT GGCAGAGTGCTGCTTGCTGC GGCAGCGTGCTGCTTGCTGC + Emx1.17-2 Single Mismatch TTGGCATCTC CCAGTTAG CCAATGGGGAGGACATCGAT GGCAGAGTGCTGCTTGCTGC GGCAGGGTGCTGCTTGCTGC + Emx1.17-3 Single Mismatch CTAGGACATTC CCAGTTAG CCAATGGGGAGGACATCGAT GGCAGAGTGCTGCTTGCTGC GGCAGTGTGCTGCTTGCTGC + Emx1.17-4 Single Mismatch CTTCCATAAC CCAGTTAG CCAATGGGGAGGACATCGAT GGCAGAGTGCTGCTTGCTGC GGCAGAGTGCAGCTTGCTGC + Emx1.17-5 Single Mismatch CCAGCCTCAAC CCAGTTAG CCAATGGGGAGGACATCGAT GGCAGAGTGCTGCTTGCTGC GGCAGAGTGCCGCTTGCTGC + Emx1.17-6 Single Mismatch CTTGGTTATTC CCAGTTAG CCAATGGGGAGGACATCGAT GGCAGAGTGCTGCTTGCTGC GGCAGAGTGCGGCTTGCTGC + Emx1.17-7 Single Mismatch TTGGCTGGAC CCAGTTAG CCAATGGGGAGGACATCGAT GGCAGAGTGCTGCTTGCTGC GGCAGAGTGCTGCTTACTGC + Emx1.17-8 Single Mismatch CCGAACACTTC CCAGTTAG CCAATGGGGAGGACATCGAT GGCAGAGTGCTGCTTGCTGC GGCAGAGTGCTGCTTCCTGC + Emx1.17-9 Single Mismatch TCCTGAATCTC CCAGTTAG CCAATGGGGAGGACATCGAT GGCAGAGTGCTGCTTGCTGC GGCAGAGTGCTGCTTTCTGC + Emx1.17-10 Single Mismatch CTAACCACGGC CCAGTTAG CCAATGGGGAGGACATCGAT GGCAGAGTGCTGCTTGCTGC GGCAGAGTGCTGCTTGCAGC + Emx1.17-11 Single Mismatch CGGAAGGATGC CCAGTTAG CCAATGGGGAGGACATCGAT GGCAGAGTGCTGCTTGCTGC GGCAGAGTGCTGCTTGCCGC + Emx1.17-12 Single Mismatch CTAGGAACCGC CCAGTTAG CCAATGGGGAGGACATCGAT GGCAGAGTGCTGCTTGCTGC GGCAGAGTGCTGCTTGCGGC + Emx1.17-13 Single Mismatch CTAAGGTAAC CCAGTTAG CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGGCCCAAGC + Emx1.18-1 Single Mismatch TAAGGAGAAC CCAGTTAG CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCAGCGTGGGCCCAAGC + Emx1.18-2 Single Mismatch AAGAGGATTC CCAGTTAG CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCCGCGTGGGCCCAAGC + Emx1.18-3 Single Mismatch TACCAAGATC CCAGTTAG CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCGGCGTGGGCCCAAGC + Emx1.18-4 Single Mismatch CAGAAGGAAC CCAGTTAG CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCATGGGCCCAAGC + Emx1.18-5 Single Mismatch CTGCAAGTTC CCAGTTAG CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCCTGGGCCCAAGC + Emx1.18-6 Single Mismatch TTCGTGATTC CCAGTTAG CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCTTGGGCCCAAGC + Emx1.18-7 Single Mismatch TTCCGATAAC CCAGTTAG CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGACCCAAGC + Emx1.18-8 Single Mismatch TGAGCGGAAC CCAGTTAG CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGCCCCAAGC + Emx1.18-9 Single Mismatch CTGACCGAAC CCAGTTAG CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGTCCCAAGC + Emx1.18-10 Single Mismatch TCCTCGAATC CCAGTTAG CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGGACCAAGC + Emx1.18-11 Single Mismatch TAGGTGGTTC CCAGTTAG CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGGGCCAAGC + Emx1.18-12 Single Mismatch TCTAACGGAC CCAGTTAG CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGGTCCAAGC + Emx1.18-13 Single Mismatch TTGGAGTGTC CCAGTTAG CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGGCCCCAGC + Emx1.18-14 Single Mismatch TCTAGAGGTC CCAGTTAG CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGGCCCGAGC + Emx1.18-15 Single Mismatch TCTGGATGAC CCAGTTAG CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGGCCCTAGC + Emx1.18-16 Single Mismatch TCTATTCGTC CCAGTTAG CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGGCCCAAAC + Emx1.18-17 Single Mismatch AGGCAATTGC CCAGTTAG CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGGCCCAACC + Emx1.18-18 Single Mismatch TTAGTCGGAC CCAGTTAG CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGGCCCAATC + Emx1.18-19 Single Mismatch CAGATCCATC CCAGTTAG CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCAGAGTCCAGCTT - Emx1.19-1 Single Mismatch TCGCAATTAC CCAGTTAG CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCAGAATCCAGCTT - Emx1.19-2 Single Mismatch TTCGAGACGC CCAGTTAG CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCAGACTCCAGCTT - Emx1.19-3 Single Mismatch TGCCACGAAC CCAGTTAG CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCAGATTCCAGCTT - Emx1.19-4 Single Mismatch AACCTCATTC CCAGTTAG CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCAGAGTCCCGCTT - Emx1.19-5 Single Mismatch CCTGAGATAC CCAGTTAG CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCAGAGTCCGGCTT - Emx1.19-6 Single Mismatch TTACAACCTC CCAGTTAG CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCAGAGTCCTGCTT - Emx1.19-7 Single Mismatch AACCATCCGC CCAGTTAG CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCAGAGTCCAGCAT - Emx1.19-8 Single Mismatch ATCCGGAATC CCAGTTAG CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCAGAGTCCAGCCT - Emx1.19-9 Single Mismatch TCGACCACTC CCAGTTAG CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCAGAGTCCAGCGT - Emx1.19-10 Single Mismatch CGAGGTTATC CCAGTTAG CTCCAATGACTAGGGTGGGC   + Emx1-18.19-Cas9 only Single Mismatch TCCAAGCTGC CCAGTTAG CTCCAATGACTAGGGTGGGC   + Emx1-18.19-no transfection Single Mismatch CGGACAATGGC CCAGTTAG GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTTCTTCATCTGCT - Emx1.13-8 Single Mismatch TTGAGCCTATTC CCAGTTAG GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTTCTTCCTCTGCT - Emx1.13-9 Single Mismatch CCGCATGGAAC CCAGTTAG GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTTCTTCGTCTGCT - Emx1.13-10 Single Mismatch CTGGCAATCCTC CCAGTTAG GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTTCTTCTACTGCT - Emx1.13-11 Single Mismatch CCGGAGAATCGC CCAGTTAG GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTTCTTCTCCTGCT - Emx1.13-12 Single Mismatch TCCACCTCCTC CCAGTTAG GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTTCTTCTGCTGCT - Emx1.13-13 Single Mismatch TCAAGAAGTTC CCAGTTAG CGAGCAGAAGAAGAAGGGCT GTGGCGCATTGCCACGAAGC GTGGCACATTGCCACGAAGC + Emx1.16-8 Single Mismatch TTCAATTGGC CCAGTTAG CGAGCAGAAGAAGAAGGGCT GTGGCGCATTGCCACGAAGC GTGGCCCATTGCCACGAAGC + Emx1.16-9 Single Mismatch CCTACTGGTC CCAGTTAG CGAGCAGAAGAAGAAGGGCT GTGGCGCATTGCCACGAAGC GTGGCTCATTGCCACGAAGC + Emx1.16-10 Single Mismatch TGAGGCTCCGAC CCAGTTAG CGAGCAGAAGAAGAAGGGCT GTGGCGCATTGCCACGAAGC GTGGCGCATTGCAACGAAGC + Emx1.16-11 Single Mismatch CGAAGGCCACAC CCAGTTAG CGAGCAGAAGAAGAAGGGCT GTGGCGCATTGCCACGAAGC GTGGCGCATTGCGACGAAGC + Emx1.16-12 Single Mismatch TCTGCCTGTC CCAGTTAG CGAGCAGAAGAAGAAGGGCT GTGGCGCATTGCCACGAAGC GTGGCGCATTGCTACGAAGC + Emx1.16-13 Single Mismatch TCTTACACAC CCAGTTAG CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTAGGCCCAAGC + Emx1.18-20 Single Mismatch TTCTCATTGAAC CCAGTTAG CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTCGGCCCAAGC + Emx1.18-21 Single Mismatch TCGCATCGTTC CCAGTTAG CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTTGGCCCAAGC + Emx1.18-22 Single Mismatch TAAGCCATTGTC CCAGTTAG CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGGCCCACGC + Emx1.18-23 Single Mismatch AAGGAATCGTC CCAGTTAG CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGGCCCAGGC + Emx1.18-24 Single Mismatch CTTGAGAATGTC CCAGTTAG CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGGCCCATGC + Emx1.18-25 Single Mismatch TGGAGGACGGAC CCAGTTAG CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGGCCCAAGA + Emx1.18-26 Single Mismatch TAACAATCGGC CCAGTTAG CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGGCCCAAGG + Emx1.18-27 Single Mismatch CTGACATAATC CCAGTTAG CTCCAATGACTAGGGTGGGC GCCCCTGCGTGGGCCCAAGC GCCCCTGCGTGGGCCCAAGT + Emx1.18-28 Single Mismatch TTCCACTTCGC CCAGTTAG CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCCGAGTCCAGCTT - Emx1.19-11 Single Mismatch AGCACGAATC CCAGTTAG CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCGGAGTCCAGCTT - Emx1.19-12 Single Mismatch CTTGACACCGC CCAGTTAG CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCTGAGTCCAGCTT - Emx1.19-13 Single Mismatch TTGGAGGCCAGC CCAGTTAG CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCAGAGACCAGCTT - Emx1.19-14 Single Mismatch TGGAGCTTCCTC CCAGTTAG CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCAGAGCCCAGCTT - Emx1.19-15 Single Mismatch TCAGTCCGAAC CCAGTTAG CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCAGAGGCCAGCTT - Emx1.19-16 Single Mismatch TAAGGCAACCAC CCAGTTAG CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCAGAGTCCAACTT - Emx1.19-17 Single Mismatch TTCTAAGAGAC CCAGTTAG CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCAGAGTCCACCTT - Emx1.19-18 Single Mismatch TCCTAACATAAC CCAGTTAG CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCAGAGTCCATCTT - Emx1.19-19 Single Mismatch CTAAGGTAAC CCAGTTAG CTGCTTGCTGCTGGCCAG GGCCTCCCCAAAGCCTGGCC GGCCTCCCCAAAGCCTGGCC - Emx1.20-1 Single Mismatch TAAGGAGAAC CCAGTTAG CTGCTTGCTGCTGGCCAG GGCCTCCCCAAAGCCTGGCC GGCCTCCCCAAAGCCTGGAC - Emx1.20-2 Single Mismatch AAGAGGATTC CCAGTTAG CTGCTTGCTGCTGGCCAG GGCCTCCCCAAAGCCTGGCC GGCCTCCCCAAAGCCTGGGC - Emx1.20-3 Single Mismatch TACCAAGATC CCAGTTAG CTGCTTGCTGCTGGCCAG GGCCTCCCCAAAGCCTGGCC GGCCTCCCCAAAGCCTGGTC - Emx1.20-4 Single Mismatch CAGAAGGAAC CCAGTTAG CTGCTTGCTGCTGGCCAG GGCCTCCCCAAAGCCTGGCC GGCCTCCCCAAAGCCAGGCC - Emx1.20-5 Single Mismatch CTGCAAGTTC CCAGTTAG CTGCTTGCTGCTGGCCAG GGCCTCCCCAAAGCCTGGCC GGCCTCCCCAAAGCCGGGCC - Emx1.20-6 Single Mismatch TTCGTGATTC CCAGTTAG CTGCTTGCTGCTGGCCAG GGCCTCCCCAAAGCCTGGCC GGCCTCCCCAAAGCCCGGCC - Emx1.20-7 Single Mismatch TTCCGATAAC CCAGTTAG CTGCTTGCTGCTGGCCAG GGCCTCCCCAAAGCCTGGCC GGCCTCCCCAACGCCTGGCC - Emx1.20-8 Single Mismatch TGAGCGGAAC CCAGTTAG CTGCTTGCTGCTGGCCAG GGCCTCCCCAAAGCCTGGCC GGCCTCCCCAAGGCCTGGCC - Emx1.20-9 Single Mismatch CTGACCGAAC CCAGTTAG CTGCTTGCTGCTGGCCAG GGCCTCCCCAAAGCCTGGCC GGCCTCCCCAATGCCTGGCC - Emx1.20-10 Single Mismatch TCCTCGAATC CCAGTTAG CTGCTTGCTGCTGGCCAG GGCCTCCCCAAAGCCTGGCC GGCCTCCCCAAAGCCAGGCC - Emx1.20-11 Single Mismatch TAGGTGGTTC CCAGTTAG CTGCTTGCTGCTGGCCAG GGCCTCCCCAAAGCCTGGCC GGCCTCCCCAAAGCCCGGCC - Emx1.20-12 Single Mismatch TCTAACGGAC CCAGTTAG CTGCTTGCTGCTGGCCAG GGCCTCCCCAAAGCCTGGCC GGCCTCCCCAAAGCCGGGCC - Emx1.20-13 Single Mismatch TTGGAGTGTC CCAGTTAG CTGCTTGCTGCTGGCCAG GGCCTCCCCAAAGCCTGGCC GGCCTCCCCAAAGCCTGGAC - Emx1.20-14 Single Mismatch TCTAGAGGTC CCAGTTAG CTGCTTGCTGCTGGCCAG GGCCTCCCCAAAGCCTGGCC GGCCTCCCCAAAGCCTGGGC - Emx1.20-15 Single Mismatch TCTGGATGAC CCAGTTAG CTGCTTGCTGCTGGCCAG GGCCTCCCCAAAGCCTGGCC GGCCTCCCCAAAGCCTGGTC - Emx1.20-16 Single Mismatch TCTATTCGTC CCAGTTAG CTGCTTGCTGCTGGCCAG   - Emx1-20-Cas9 only Single Mismatch AGGCAATTGC CCAGTTAG CTGCTTGCTGCTGGCCAG   - Emx1-20-no transfection Single Mismatch CAGCATTAATTC CCAGTTAG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA GAGACCGAGCAGAAGAAGAA + Emx1.3-1 Single Mismatch TCTGGCAACGGC CCAGTTAG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA GAGCCCGAGCAGAAGAAGAA + Emx1.3-2 Single Mismatch TCCTAGAACAC CCAGTTAG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA GAGGCCGAGCAGAAGAAGAA + Emx1.3-3 Single Mismatch TCCTTGATGTTC CCAGTTAG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA GAGTCCGAGAAGAAGAAGAA + Emx1.3-4 Single Mismatch TCTAGCTCTTC CCAGTTAG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA GAGTCCGAGGAGAAGAAGAA + Emx1.3-5 Single Mismatch TCACTCGGATC CCAGTTAG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA GAGTCCGAGTAGAAGAAGAA + Emx1.3-6 Single Mismatch TTCCTGCTTCAC CCAGTTAG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA GAGTCCGAGCCGAAGAAGAA + Emx1.3-7 Single Mismatch CCTTAGAGTTC CCAGTTAG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA GAGTCCGAGCGGAAGAAGAA + Emx1.3-8 Single Mismatch CTGAGTTCCGAC CCAGTTAG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA GAGTCCGAGCTGAAGAAGAA + Emx1.3-9 Single Mismatch TTAGTCGGAC CCAGTTAG CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGGCACAGCTGAGAAACTC - Emx1.10-17 Single Mismatch CAGATCCATC CCAGTTAG CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGGCACAGGTGAGAAACTC - Emx1.10-18 Single Mismatch TCGCAATTAC CCAGTTAG CCTCAGTCTTCCCATCAGGC GGGGCACAGATGAGAAACTC GGGGCACAGTTGAGAAACTC - Emx1.10-19 Single Mismatch CGGACAATGGC CCAGTTAG TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGATGGAGGAGGAA + Emx1.12-35 Single Mismatch TTGAGCCTATTC CCAGTTAG TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGGTGGAGGAGGAA + Emx1.12-36 Single Mismatch CCGCATGGAAC CCAGTTAG TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGTTGGAGGAGGAA + Emx1.12-37 Single Mismatch CTGGCAATCCTC CCAGTTAG TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCAGGAGGAGGAA + Emx1.12-38 Single Mismatch CCGGAGAATCGC CCAGTTAG TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCCGGAGGAGGAA + Emx1.12-39 Single Mismatch TCCACCTCCTC CCAGTTAG TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCGGGAGGAGGAA + Emx1.12-40 Single Mismatch CAGCATTAATTC CCAGTTAG TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGAGAAGGAA + Emx1.12-41 Single Mismatch TCTGGCAACGGC CCAGTTAG TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGAGCAGGAA + Emx1.12-42 Single Mismatch TCCTAGAACAC CCAGTTAG TCATCTGTGCCCCTCCCTC GGCAGAAGCTGGAGGAGGAA GGCAGAAGCTGGAGTAGGAA + Emx1.12-43 Single Mismatch TCCTGGCACATC CCAGTTAG GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTACTTCTTCTGCT - Emx1.13-14 Single Mismatch CCGCAATCATC CCAGTTAG GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTCCTTCTTCTGCT - Emx1.13-15 Single Mismatch TTCCTACCAGTC CCAGTTAG GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTGCTTCTTCTGCT - Emx1.13-16 Single Mismatch TCAAGAAGTTC CCAGTTAG GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTTATTCTTCTGCT - Emx1.13-17 Single Mismatch TTCAATTGGC CCAGTTAG GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTTGTTCTTCTGCT - Emx1.13-18 Single Mismatch CCTACTGGTC CCAGTTAG GGAGGACAAAGTACAAACGGC GGAGCCCTTCTTCTTCTGCT GGAGCCCTTTTTCTTCTGCT - Emx1.13-19 Single Mismatch CGGACAATGGC CCAGTTAG CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCCGAGTCCAGCTT - Emx1.19-20 Single Mismatch TTGAGCCTATTC CCAGTTAG CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCGGAGTCCAGCTT - Emx1.19-21 Single Mismatch CCGCATGGAAC CCAGTTAG CTCCAATGACTAGGGTGGGC GAGTGGCCAGAGTCCAGCTT GAGTGGCCTGAGTCCAGCTT - Emx1.19-22 Single Mismatch TCCTGGCACATC CCAGTTAG GGAGGACAAAGTACAAACGGC GAGTCCGAGCAGAAGAAGAA GAGTCCGAGCAGAAGAAGAA + Emx1.3-no mutation Single Mismatch CTAAGGTAAC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-1 Multiple Mismatch TAAGGAGAAC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-2 Multiple Mismatch AAGAGGATTC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-3 Multiple Mismatch TACCAAGATC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-4 Multiple Mismatch CAGAAGGAAC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-5 Multiple Mismatch CTGCAAGTTC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-6 Multiple Mismatch TTCGTGATTC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-7 Multiple Mismatch TTCCGATAAC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-8 Multiple Mismatch TGAGCGGAAC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-9 Multiple Mismatch CTGACCGAAC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-10 Multiple Mismatch TCCTCGAATC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-11 Multiple Mismatch TAGGTGGTTC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-12 Multiple Mismatch TCTAACGGAC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-13 Multiple Mismatch TTGGAGTGTC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-14 Multiple Mismatch TCTAGAGGTC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-15 Multiple Mismatch TCTGGATGAC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-16 Multiple Mismatch TCTATTCGTC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-17 Multiple Mismatch AGGCAATTGC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-18 Multiple Mismatch TTAGTCGGAC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-19 Multiple Mismatch CAGATCCATC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-20 Multiple Mismatch TCGCAATTAC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-21 Multiple Mismatch TTCGAGACGC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-22 Multiple Mismatch TGCCACGAAC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-23 Multiple Mismatch AACCTCATTC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-24 Multiple Mismatch CCTGAGATAC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-25 Multiple Mismatch TTACAACCTC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-26 Multiple Mismatch AACCATCCGC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-27 Multiple Mismatch ATCCGGAATC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-28 Multiple Mismatch TCGACCACTC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-29 Multiple Mismatch CGAGGTTATC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-30 Multiple Mismatch TCCAAGCTGC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-31 Multiple Mismatch TCTTACACAC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-32 Multiple Mismatch TTCTCATTGAAC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-33 Multiple Mismatch TCGCATCGTTC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-34 Multiple Mismatch TAAGCCATTGTC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-35 Multiple Mismatch AAGGAATCGTC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-36 Multiple Mismatch CTTGAGAATGTC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-37 Multiple Mismatch TGGAGGACGGAC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-38 Multiple Mismatch TAACAATCGGC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-39 Multiple Mismatch CTGACATAATC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-40 Multiple Mismatch TTCCACTTCGC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-41 Multiple Mismatch AGCACGAATC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-42 Multiple Mismatch CTTGACACCGC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-43 Multiple Mismatch TTGGAGGCCAGC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-44 Multiple Mismatch TGGAGCTTCCTC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-45 Multiple Mismatch TCAGTCCGAAC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-46 Multiple Mismatch TAAGGCAACCAC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-47 Multiple Mismatch TTCTAAGAGAC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-48 Multiple Mismatch TCCTAACATAAC GGTCCAGA CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-49 Multiple Mismatch CGGACAATGGC GGTCCAGA CCAATGGGGAGGACATCGAT   + Emx1.1-50 Multiple Mismatch TTGAGCCTATTC GGTCCAGA CCAATGGGGAGGACATCGAT   + Emx1.1-51 Multiple Mismatch CTAAGGTAAC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-1 Multiple Mismatch TAAGGAGAAC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-2 Multiple Mismatch AAGAGGATTC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-3 Multiple Mismatch TACCAAGATC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-4 Multiple Mismatch CAGAAGGAAC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-5 Multiple Mismatch CTGCAAGTTC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-6 Multiple Mismatch TTCGTGATTC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-7 Multiple Mismatch TTCCGATAAC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-8 Multiple Mismatch TGAGCGGAAC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-9 Multiple Mismatch CTGACCGAAC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-10 Multiple Mismatch TCCTCGAATC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-11 Multiple Mismatch TAGGTGGTTC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-12 Multiple Mismatch TCTAACGGAC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-13 Multiple Mismatch TTGGAGTGTC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-14 Multiple Mismatch TCTAGAGGTC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-15 Multiple Mismatch TCTGGATGAC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-16 Multiple Mismatch TCTATTCGTC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-17 Multiple Mismatch AGGCAATTGC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-18 Multiple Mismatch TTAGTCGGAC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-19 Multiple Mismatch CAGATCCATC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-20 Multiple Mismatch TCGCAATTAC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-21 Multiple Mismatch TTCGAGACGC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-22 Multiple Mismatch TGCCACGAAC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-23 Multiple Mismatch AACCTCATTC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-24 Multiple Mismatch CCTGAGATAC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-25 Multiple Mismatch TTACAACCTC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-26 Multiple Mismatch AACCATCCGC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-27 Multiple Mismatch ATCCGGAATC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-28 Multiple Mismatch TCGACCACTC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-29 Multiple Mismatch CGAGGTTATC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-30 Multiple Mismatch TCCAAGCTGC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-31 Multiple Mismatch TCTTACACAC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-32 Multiple Mismatch TTCTCATTGAAC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-33 Multiple Mismatch TCGCATCGTTC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-34 Multiple Mismatch TAAGCCATTGTC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-35 Multiple Mismatch AAGGAATCGTC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-36 Multiple Mismatch CTTGAGAATGTC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-37 Multiple Mismatch TGGAGGACGGAC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-38 Multiple Mismatch TAACAATCGGC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-39 Multiple Mismatch CTGACATAATC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-40 Multiple Mismatch TTCCACTTCGC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-41 Multiple Mismatch AGCACGAATC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-42 Multiple Mismatch CTTGACACCGC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-43 Multiple Mismatch TTGGAGGCCAGC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-44 Multiple Mismatch TGGAGCTTCCTC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-45 Multiple Mismatch TCAGTCCGAAC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-46 Multiple Mismatch TAAGGCAACCAC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-47 Multiple Mismatch TTCTAAGAGAC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-48 Multiple Mismatch TCCTAACATAAC GGTCCAGA AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-49 Multiple Mismatch CGGACAATGGC GGTCCAGA AGAAGCTGGAGGAGGAAGGG   - Emx1.6-50 Multiple Mismatch TTGAGCCTATTC GGTCCAGA AGAAGCTGGAGGAGGAAGGG   - Emx1.6-51 Multiple Mismatch CTAAGGTAAC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-1 Multiple Mismatch TAAGGAGAAC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-2 Multiple Mismatch AAGAGGATTC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-3 Multiple Mismatch TACCAAGATC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-4 Multiple Mismatch CAGAAGGAAC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-5 Multiple Mismatch CTGCAAGTTC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-6 Multiple Mismatch TTCGTGATTC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-7 Multiple Mismatch TTCCGATAAC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-8 Multiple Mismatch TGAGCGGAAC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-9 Multiple Mismatch CTGACCGAAC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-10 Multiple Mismatch TCCTCGAATC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-11 Multiple Mismatch TAGGTGGTTC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-12 Multiple Mismatch TCTAACGGAC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-13 Multiple Mismatch TTGGAGTGTC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-14 Multiple Mismatch TCTAGAGGTC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-15 Multiple Mismatch TCTGGATGAC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-16 Multiple Mismatch TCTATTCGTC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-17 Multiple Mismatch AGGCAATTGC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-18 Multiple Mismatch TTAGTCGGAC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-19 Multiple Mismatch CAGATCCATC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-20 Multiple Mismatch TCGCAATTAC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-21 Multiple Mismatch TTCGAGACGC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-22 Multiple Mismatch TGCCACGAAC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-23 Multiple Mismatch AACCTCATTC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-24 Multiple Mismatch CCTGAGATAC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-25 Multiple Mismatch TTACAACCTC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-26 Multiple Mismatch AACCATCCGC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-27 Multiple Mismatch ATCCGGAATC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-28 Multiple Mismatch TCGACCACTC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-29 Multiple Mismatch CGAGGTTATC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-30 Multiple Mismatch TCCAAGCTGC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-31 Multiple Mismatch TCTTACACAC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-32 Multiple Mismatch TTCTCATTGAAC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-33 Multiple Mismatch TCGCATCGTTC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-34 Multiple Mismatch TAAGCCATTGTC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-35 Multiple Mismatch AAGGAATCGTC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-36 Multiple Mismatch CTTGAGAATGTC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-37 Multiple Mismatch TGGAGGACGGAC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-38 Multiple Mismatch TAACAATCGGC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-39 Multiple Mismatch CTGACATAATC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-40 Multiple Mismatch TTCCACTTCGC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-41 Multiple Mismatch AGCACGAATC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-42 Multiple Mismatch CTTGACACCGC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-43 Multiple Mismatch TTGGAGGCCAGC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-44 Multiple Mismatch TGGAGCTTCCTC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-45 Multiple Mismatch TCAGTCCGAAC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-46 Multiple Mismatch TAAGGCAACCAC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-47 Multiple Mismatch TTCTAAGAGAC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-48 Multiple Mismatch TCCTAACATAAC TCGGAATG CCAATGGGGAGGACATCGAT GTCACCTCCAATGACTAGGG  + Emx1.1-49 Multiple Mismatch CGGACAATGGC TCGGAATG CCAATGGGGAGGACATCGAT   + Emx1.1-50 Multiple Mismatch TTGAGCCTATTC TCGGAATG CCAATGGGGAGGACATCGAT   + Emx1.1-51 Multiple Mismatch CTAAGGTAAC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-1 Multiple Mismatch TAAGGAGAAC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-2 Multiple Mismatch AAGAGGATTC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-3 Multiple Mismatch TACCAAGATC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-4 Multiple Mismatch CAGAAGGAAC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-5 Multiple Mismatch CTGCAAGTTC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-6 Multiple Mismatch TTCGTGATTC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-7 Multiple Mismatch TTCCGATAAC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-8 Multiple Mismatch TGAGCGGAAC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-9 Multiple Mismatch CTGACCGAAC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-10 Multiple Mismatch TCCTCGAATC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-11 Multiple Mismatch TAGGTGGTTC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-12 Multiple Mismatch TCTAACGGAC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-13 Multiple Mismatch TTGGAGTGTC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-14 Multiple Mismatch TCTAGAGGTC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-15 Multiple Mismatch TCTGGATGAC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-16 Multiple Mismatch TCTATTCGTC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-17 Multiple Mismatch AGGCAATTGC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-18 Multiple Mismatch TTAGTCGGAC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-19 Multiple Mismatch CAGATCCATC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-20 Multiple Mismatch TCGCAATTAC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-21 Multiple Mismatch TTCGAGACGC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-22 Multiple Mismatch TGCCACGAAC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-23 Multiple Mismatch AACCTCATTC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-24 Multiple Mismatch CCTGAGATAC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-25 Multiple Mismatch TTACAACCTC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-26 Multiple Mismatch AACCATCCGC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-27 Multiple Mismatch ATCCGGAATC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-28 Multiple Mismatch TCGACCACTC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-29 Multiple Mismatch CGAGGTTATC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-30 Multiple Mismatch TCCAAGCTGC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-31 Multiple Mismatch TCTTACACAC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-32 Multiple Mismatch TTCTCATTGAAC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-33 Multiple Mismatch TCGCATCGTTC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-34 Multiple Mismatch TAAGCCATTGTC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-35 Multiple Mismatch AAGGAATCGTC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-36 Multiple Mismatch CTTGAGAATGTC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-37 Multiple Mismatch TGGAGGACGGAC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-38 Multiple Mismatch TAACAATCGGC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-39 Multiple Mismatch CTGACATAATC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-40 Multiple Mismatch TTCCACTTCGC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-41 Multiple Mismatch AGCACGAATC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-42 Multiple Mismatch CTTGACACCGC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-43 Multiple Mismatch TTGGAGGCCAGC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-44 Multiple Mismatch TGGAGCTTCCTC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-45 Multiple Mismatch TCAGTCCGAAC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-46 Multiple Mismatch TAAGGCAACCAC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-47 Multiple Mismatch TTCTAAGAGAC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-48 Multiple Mismatch TCCTAACATAAC TCGGAATG AGAAGCTGGAGGAGGAAGGG GCGCCACCGGTTGATGTGAT  - Emx1.6-49 Multiple Mismatch CGGACAATGGC TCGGAATG AGAAGCTGGAGGAGGAAGGG   - Emx1.6-50 Multiple Mismatch TTGAGCCTATTC TCGGAATG AGAAGCTGGAGGAGGAAGGG   - Emx1.6-51 Multiple Mismatch 