GSM1466899: ΔznuA; Pseudomonas aeruginosa PAO1; RNA-Seq
Cells were collected and lysed using TRIzol/Chloroform. Subsequent RNA extraction was performed using an Ambion PureLink RNA Isolation kit. RNA was treated with DNase I (Roche) and cleanup on a Ambion PureLink RNA isolation column. Ribosomal RNA was removed using RiboZero Bacterial (Gram-) depletion kit (Illumina). The RNA integrity was assessed on a BioAnalyzer (RIN > 9). Biological replicates were pooled. Illumina TruSeq Stranded Total RNA LT Sample Prep Kit.
GEO Accession
GSM1466899