GSM4761233: HEPG2.IL1B.23h.rep1; Homo sapiens; ChIP-Seq
ChIP-Seq: Formaldehyde (10′) fixed lysates were clarified from sonicated nuclei and protein–DNA complexes were isolated with H3K27ac antibody (ab4729, Abcam) ChIP-Seq: DNA was end-repaired using a combination of T4 DNA polymerase, Escherichia coli DNA Pol I large fragment (Klenow polymerase; New England Biolabs) and PNK (Enzymatics Inc., Beverly, MA, USA). The blunt, phosphorylated ends were treated with Klenow fragment (minus exo; New England Biolabs) and dATP to yield a protruding 3- 'A' base for ligation of Illumina's adapters that have a single 'T' base overhang at the 3′ end. After adapter ligation, ChIP DNA was polymerase chain reaction (PCR) amplified with Illumina genomic adaptors or with NEXTflex DNA barcode adaptors (Bioo Scientific Corporation, Austin, TX, USA) and library fragments were size-selected (150–250 bp) from a 2% agarose gel. All libraries were sequenced on Illumina HiSeq 2000 according to the manufacturer's instructions.
GEO Accession
GSM4761233