NextSeq 500 paired end sequencing; Blood RNAseq of TB contacts samples
Blood RNAseq of TB contacts samples
Contacts of patients with active pulmonary and extrapulmonary TB, with a cumulative duration of exposure of greater than eight hours in a confined space to the index case prior to initiation of treatment, were invited to participate from multiple UK TB clinics.  Total blood RNA was extracted using the Tempus Spin RNA Isolation Kit (Ambion; Life Technologies). Globin mRNA and genomic DNA were removed using the GlobinClear kit (Life Technologies) and the TURBO DNA-free kit (Ambion, Life Technologies) respectively cDNA libraries were generated using the Kappa Hyperprep kit (Roche)
Experimental Factor: clinical information
Interferon Gamma Release Assay (IGRA) positive