Whole-genome sequencing of Campylobacter jejuni
Genomic DNA was prepared from bacteria grown on blood agar medium (Fort Richard, Auckland, NZ) using a Wizard Genomic DNA Purification Kit (Promega) according to the manufacturer's instructions. A library for unidirectional sequencing was prepared from 5 g genomic DNA for each isolate using a single end DNA Sample Prep Kit (Part Number FC-102-1001, Illumina Inc). The genomic DNA was fragmented by nebulisation for 6 minutes at a pressure of 32 psi, end repaired, tailed, adaptor-ligated, fractionated, purified and enriched according to the manufacturer's instructions. A flow cell was prepared for each isolate using the Single Read Cluster Generation Kit (Part Number GD-203-1001, Illumina Inc) and a Cluster Station. Sequencing reactions using 4 pmoles of the library were performed on an Illumina Genome Analyser instrument with a single read 51 cycle SBS sequencing kit (Illumina Inc).