T.gamsii self-interaction R1
Libraries were prepared by using the TruSeq stranded mRNA kit (Illumina) starting from 900 ng of total RNA for each sample and following the manufacturers instruction. Libraries were quantified using Qubit fluorometer (Thermo Fisher Scientific) and the quality checked by 2100 Bioanalyzer (Agilent Technologies). Illumina NextSeq500 sequencer platform (75bp paired-end reads) was used for RNA sequencing that was performed at the BMR Genomics (Padova, Italy)