Pooled sample consisting of samples: Cyst 702 S1|Cyst 704 S1|Cyst 705 S1|Cyst 715 S1|Cyst 721 S1|Cyst 727 S1|Cyst 738 S1|Cyst 742 S1|Cyst 745 S1|Cyst 749 S1|Cyst 750 S1|Cyst 763 S1|Cyst 766 S1|Cyst 780 S1|Cyst 786 S1|Cyst 787 S1|Cyst 788 S1
Primers were used to amplify DNA in 25-uL multiplex polymerase chain reactions (PCRs) as described previously 15.  For each sample, three multiplex PCRs were performed, with each multiplex PCR containing 22 to 50 primer pairs.  Reactions were purified with AMPure XP beads (Beckman Coulter) and eluted in 100 uL of Buffer EB (Qiagen, cat. no. 19086).  0.25% of the purified PCR products were then amplified in a second round of PCR, as described in Springer et al.15.  The PCR products were purified with AMPure and used for sequencing on a MiSeq or HiSeq instrument