WGS of Mycoplasma mucosicanis: ATCC BAA-1895
The Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA) was used to prepare fragment libraries of the Mycoplasma cynos and Mycoplasma mucosicanis DNA for whole genome sequencing; this was performed according to the manufacturers instructions. Tagmented libraries were purified using AMPure XP Beads (Beckman Coulter Life Sciences, Indianapolis, IN) at a concentration of 0.5x. Barcoded  whole genome libraries were then pooled and run on Illumina MiSeq Reagent Kit (V3) cartridge at 2x250 cycles using a MiSeq next-generation sequencer.