GSM4698737: MC1; Mus musculus; RNA-Seq
Total RNA (including miRNA) from blood was isolated using RNeasy Protect Animal Blood Kit (Cat No./ID: 73224, Qiagen) with Buffer RWT (cat. no. 1067933, Qiagen). Colon total RNA was isolated using miRNeasy Mini Kit (Cat No./ID: 217004, Qiagen) after simultaneous tissue disruption and homogenization using TissueLyser LT (Cat No./ID: 85600, Qiagen) and Stainless-Steel Beads, 5 mm (Cat No./ID: 69989, Qiagen). Library preparation for colon RNA samples was performed using Ribo-Zero™ Magnetic Gold rRNA Removal Kit (Epidemiology, MRZE724, illumina) and for blood, using Globin-Zero Gold rRNA Removal Kit (GZG1224, illumine) based on the standard kits protocol.
GEO Accession
GSM4698737