HiSeq of Enterovirus D68: patient sample
The DNA preparations were quantified using QuantiTTM dsDNA High-Sensitivity Assay Kit and Tecan Spark 10M plate reader. 1 l of DNA (approximately 0.52.0 ng/l) was used in the tagmentation reaction using Nextera chemistry (Illumina) to yield fragments larger than 150 bp. The tagmented library underwent 11 cycles of PCR with single-end indexed primers (IDT Technologies) followed by purification using Seramag beads according to the protocol. The library was quantified using Quant-iTTM dsDNA High-Sensitivity Assay Kit and Tecan Spark 10M plate reader and pair-end (2101 bp) sequenced to a depth of 100,000  1,000,000 reads per sample on a HiSeq 2500 Illumina sequencer. Base calling and demultiplexing was done using bcl2fastq v1.87, without allowing any mismatch in the index sequence.