Zea mays: S46 control rep 1
Total RNAs were extracted from the leaf of R9 and S46 at 7 and 12 days post salinity usingRNX-Plus TM extraction solution (Sinaclon, Iran) according to the manufacturer's recommendation followed by DNase digestion. After quality control procedures using gel electrophoresis, nanodrop, and Agilent 2100 bioassays, mRNA was purified. Equal quantitative of total RNA from three biological replicates from each time point (7 and 12 days after salinity stress) were then pooled for following RNA sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols.