GSM1185390: sRNAs from SINV-infected VERO cells; Chlorocebus aethiops; RNA-Seq
RNA was extracted from non-infected and infected cells using Tri-reagent (Ambion) as per manufacturer’s instructions. Small RNA libraries were prepared from 10 or 20 μg of total RNA as previously described (Pfeffer, S. 2007. Identification of virally encoded microRNAs. Methods Enzymol. 427:51-63), except that PCR products were not concatemerized but directly sent for large-scale sequencing. Libraries from samples 5 to 10 were generated using the same protocol except that degenerate 5' and 3' adapters were used to limit ligation biases.
GEO Accession
GSM1185390