Illumina HiSeq 2000 paired end sequencing; MeDIP profiling by high-throughput sequencing of Primary Human Hepatocytes (PHH) exposed to Valproic Acid (VPA) for 5 days daily and 5 days daily exposure followed by 3 days washout.
MeDIP profiling by high-throughput sequencing of Primary Human Hepatocytes (PHH) exposed to Valproic Acid (VPA) for 5 days daily and 5 days daily exposure followed by 3 days washout.
Primary human hepatocytes (PHH) were exposed daily for 5 days to 15 mM VPA after which DNA was isolated. After terminating the VPA treatment, part of the cells were kept with medium for 3 days (washout-period (WO-period)) in order to investigate the eventual persistence of VPA-induced methylome changes. Starting from the Methylated DNA samples, methylated DNAs were size selected and ligated for sequencing following the TruSeqTM SBS Kit v3 paired-end by sequencing by synthesis (SBS)
Experimental Factor: 8
time
Experimental Factor: Valproic Acid
compound
Experimental Factor: 15
dose