Identification and characterization of microRNA-size, small RNAs in candida albicans.
Total RNA was extracted from the cultured C. albicans after pooling using QIAzol solution (QIAGEN, Valencia, CA, USA) according to the manufacturer’s protocol. RNAs were processed and used for deep sequencing by LC Sciences (Houston, TX, USA), as previously described (Lee and Hong, 2012). The purified small-sized cDNA library was used for cluster generation on Illumina’s (San Diego, CA, USA) Cluster Station and then sequenced on Illumina GAIIx following the vendor’s instructions for running the instrument.