Whole-genome sequening of the Caenorhabditis tropicalis strain INK77 carrying abuIR4(V:11.9-13.5 Mb, EG6180 > NIC203)
DNA was extracted using the Masterpure Complete DNA and RNA Purification Kit, and WGS libraries were generated using a library prep protocol similar to the Illumina Nextera Library Prep Kit, but uses a homebrew version of the TN5 transposase carrying mutations described in the following reference: Kia, A., Gloeckner, C., Osothprarop, T. et al. Improved genome sequencing using an engineered transposase. BMC Biotechnol 17, 6 (2017).