RNA-Seq of Human paraganglioma patient female
The library preparation was performed using a TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold (Illumina, USA). This method allows removing both cytoplasmic and mitochondrial rRNA using biotinylated probes that selectively bind rRNA species. Magnetic beads capture the probe-rRNA hybrid and are removed by pulldown, leaving the desired rRNA-depleted RNA in solution. This process minimizes ribosomal contamination and maximizes the percentage of uniquely mapped reads covering both mRNA and a broad range of ncRNA species of interest.