GSM4711209: Trachea.V.1; Homo sapiens; RNA-Seq
Total RNA in the cells was extracted using Trizol (Invitrogen) according to the manufacturer's protocol, and 1ug RNA was used to reverse transcribed into cDNA using Oligo (dT). Fragmented RNA (average length approximately 200 bp) was subjected to first strand and second strand cDNA synthesis followed by adaptor ligation and enrichment with a low-cycle according to the instructions of NEBNext" UltraTM RNA Library Prep Kit for Illumina (NEB, USA). The purified library products were evaluated using the Agilent 2200 TapeStation and Qubit"2.0(Life Technologies, USA).
GEO Accession
GSM4711209