GSM1186142: DA; Apostichopus japonicus; RNA-Seq
Total RNA from each tissue sample was extracted using miRNeasy Mini kit (Qiagen) according to the manufacturer’s instructions. Recovered Small RNAs were ligated sequentially to 5’ and 3’ adapters. Reverse transcription was performed followed by PCR amplification. The purified PCR products were used directly for cluster generation and sequencing analysis using the Illumina’s Solexa Sequencer according to the manufacturer’s instrunctions
GEO Accession
GSM1186142