Renseq analysis of transgenic wheat line P52 which carries a Sr22_Sr35_Sr45_Sr50_Sr55 multi-transgene cassette
RNA was extracted from wheat seedlings at the fourth leaf stage and cDNA produced using a SuperScript III Reverse Transcriptase kit (Invitrogen, USA). DNA adapters were added to cDNAs and NLR sequences enriched using RenSeq exome capture with Tv2 baits and 125PE sequencing performed in HiSeq2500 platform by Arbor Biosciences (Ann Abor, USA).