Genome of B. megaterium
The library was prepared using Nextera DNA Flex library preparation kit (Illumina) following the manufacturer's user guide. 50 ng DNA was used to prepare the library. The sample underwent the simultaneous fragmentation and addition of adapter sequences. These adapters are utilized during a limited-cycle (6 cycles) PCR in which unique index was added to the sample. Following the library preparation, the final concentration of the library (Table 1) was measured using the Qubit dsDNA HS Assay Kit (Life Technologies), and the average library size (Table 1) was determined using the Agilent 2100 Bioanalyzer (Agilent Technologies).  The library was diluted (to 0.9nM) and sequenced paired end for 500 cycles using the NovaSeq system (Illumina)