GSM4251578: A549 cells, Cal07_Infected; Homo sapiens; RNA-Seq
A549 cell monolayers were trypsinized into single-cell suspension, stained with anti-HA and anti-M2 monoclonal antibody, washed with PBS, and sorted out based on the expression of HA and M2. Each cell sample was used to generate individually barcoded cDNA libraries using the 10x Chromium Single Cell 3' platform (Pleasanton, CA) following the manufacturer's protocol. Individually barcoded cDNA libraries using the 10x Chromium Single Cell 3' platform (Pleasanton, CA) were generated following the manufacturer's protocol. The cDNA libraries were then prepared for standard Illumina sequencing. Cal07 used V2 chemistry and Perth09 used V3 chemistry
GEO Accession
GSM4251578