Rat small intestine
To develop intestinal cells derived from human embryonic stem cells as small intestine models, the small intestine of rats, the currently used in vivo model, and the cells were sequenced. To perform RNA-seq, total RNA was extracted from the entire layers of the proximal part of the small intestine (5 cm from pylorus) and the cells. cDNA libraries were generated according to standard procedures using the Lexogen QuantSeq 3' mRNA-Seq Library Prep Kit (Vienna, Austria) and the libraries were sequenced on an Illumina NextSeq500 (San Diego, CA, USA) in single-end (SE) 75-base mode.