PolyA-prepped mRNA from Dicer KO
PolyA-collected mRNA was obtained via the Qiagen miRNeasy kit from subconfluent WT and KO fibroblasts that were lysed using QIAzol directly in the dish.  The Illumina Tru-Seq kit was used for sequence preparation, which began with an input amount of 1 µg total RNA with a RIN above 9.0. The final libraries were size-selected to 300 +/- 25 bp on a 2% agarose gel for (50/50) paired end sequencing on an Illumina HiSeq 2000 machine