Whole transcriptome analysis on WT SH-SY5Y cell line
Whole Trascriptome analysis was performed by RNA-seq technology on WT and sacsin KO SH-SY5Y cells. Cells were collected in three indipendent experiments and total RNA was isolated using TripleXtractor. RNA purity, quantity and integrity were checked before to proceed with the library preparation. Directional RNA-seq libraries were prepared from 1500ng total RNA using the TruSeq Stranded kit (Illumina) after poly(A) capture.