GSM4251633: CTRL rep1 [ATAC-seq]; Mus musculus; ATAC-seq
Neurospheres cultured for 7 days were digested by trypsin and dissociated. The suspended cells (5*10^5/sample) were washed with cold PBS, collected by centrifugation then resuspended in resuspension buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2). After collection, cells were lysed in lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% NP-40) and collected before incubating in transposition mix containing Tn5 transposase. The Tn5 transposase reaction and library construction is prepared using TruePrepTM DNA Library Prep Kit V2 for Illumina (Vazyme, TD501).The purified library was analyzed on an Agilent Bioanalyzer, and single-read sequencing was performed using Illumina HiseqXten-PE150 platform according to standard operating procedures.
GEO Accession
GSM4251633