GSM4209617: Sample_6_ASF_MUC_2; Mus musculus; RNA-Seq
IEC were collected in Trizol and lysed by repeated passing through a 27G syringe needle. RNA was extracted by chloroform phase separation followed by RNA extraction with in-column DNase treatment using the Direct-zol RNA Kit Poly-A pull-down was performed to enrich mRNAs from total RNA samples, followed by library construction using Illumina TruSeq chemistry. Cluster generation and 50–base pair single-end 50 cycled sequencing was performed on the Illumina HiSeq4000 system
GEO Accession
GSM4209617