GSM2792377: RNAP ChIP, High Light Pulse, 8.25 hours since Dawn, Time 15 min, replicate 2; Synechococcus elongatus PCC 7942; ChIP-Seq
120 mL of OD750 0.3 culture was crosslinked in 1% formaldehyde. Cells were lysed and fragmented genomic lysates were prepared using a Covaris acoustic sonication device. Protein-DNA complexes were isolated using antibody. Sequencing libraries were prepared from purified ChIP DNA using the NEBNext Ultra II DNA Library Prep Kit.
GEO Accession
GSM2792377