GSM1831849: GRBC_CLIP_control2; Trypanosoma brucei; RNA-Seq
Mitochondrial fraction was isolated as described except omitting the Percoll density gradient (Pelletier et al., 2007). Mitochondrial pellets were extracted into 25 mM HEPES (pH 7.6), 125 mM KCl, 12 mM MgCl2 and 1.2 % Nonidet P-40 (NP-40) and fractionated on 10%-30% glycerol gradient. RNA was excised from areas corresponding to 35 to 75 nucleotides (nt), eluted, and processed with a ScriptMiner small RNA-Seq library preparation kit (Epicentre) to generate Illumina-compatible libraries.Single-ended 75-nt stranded sequencing and raw data extraction were performed at the University of California (UC) Irvine Genomics High-Throughput Facility.
GEO Accession
GSM1831849