Prokaryotic community composition of Lake Ferto open water sediment sample collected on 18 Jul 2016 based on 16S rRNA gene amplicons
A part of the 16S rRNA gene was amplified with fusion primers containing Bacteria-specific sequences Bakt_341F (5'-CCT ACG GGN GGC WGC AG-3', Klindworth et al. 2013) and modified Bakt_805R (5'-GAC TAC NVG GGT ATC TAA TCC-3', Herlemann et al. 2011), and Archaea-specific A519F (5CAG CMG CCG CGG TAA3, Klindworth et al. 2013) and Arch855R  (5TCC CCC GCC AAT TCC TTT AA  3, Klindworth et al. 2013) with Fluidigm CS1 and CS2 universal sequences at their 5' ends. The resulting PCR products were mixed before sequencing in 1:1 ratio.