GSM4209496: mC_brain_arctic_lamprey; Lethenteron camtschaticum; Bisulfite-Seq
DNeasy Blood & Tissue kit (Qiagen) Genomic DNA was fragmented with a Covaris S2 sonicator to a mean length of 200 bp, then end-repaired, A-tailed, ligated to methylated Illumina TruSeq adapters, bisulfite converted and subjected to 7 cycles of PCR amplification with KAPA HiFi Uracil+ DNA polymerase (KAPA Biosystems).
GEO Accession
GSM4209496