GSM3204773: met-1_mes-4_rep2; Caenorhabditis elegans; RNA-Seq
L1s were lysed in 750μl Trizol® and snap-frozen in liquid nitrogen. After freeze cracking of the samples by 6 subsequent transfers from liquid nitrogen to 42°C, samples were let vigorously shake 15 min at RT. Total RNA was purified using RNeasy kit (QIAGEN), including DNase treatment. RNA-seq libraries were obtained using the Illumina ScriptSeq Complete Gold kit according to manufacturer's guide, starting with 5µg of total RNA as input
GEO Accession
GSM3204773