DNA gut microbiota
Total DNA was extracted from each stool using the QIAamp Fast DNA Stool Mini kit (Qiagen), quantified using a Synergy HT spectrophotometer (Biotek) and stored at -20C. DNA samples were shipped to Molecular Research LP (TX, USA) for DNA amplification and sequencing of the V3-V4 regions of the 16S rRNA, using the Illumina miSeq 2x300 PE.