RNaseq of Saccharomyces cerevisiae Y06240: evolved in YPD for  110 passages, challenged in YPD, replica 2
Total RNA was depleted of ribosomal  RNA using Ribo-Zero Gold rRNA removal yeast kit (Illumina, WI). Indexed  libraries RNAseq libraries were constructed using strand-specific cDNA  synthesis (TruSeq stranded mRNA preparation kit, Illumina) from oligo-dT captured  mRNAs, pooled in equimolar concentration and subjected to single-end 75bp  Illumina sequencing in an Illumina NextSeq500 platform using High Output 75  cycles kit v2.0 (illumina). All performed at the Genomic core facility at  SCSIE from University of Valencia (Valencia, Spain)