GSM4710394: scRNA-seq E14.5 somatosensory cortex rep2; Mus musculus; RNA-Seq
The somatosensory cortex of E14.5 mouse embryos was isolated and meninges removed. Single cell suspension was prepared using the papain-based neural dissociation kit (Miltenyi Biotec, Cat. N: 130-092-628) according to the manufacturer protocol with reduced dissociation times (5 min. instead of 15 min. at 37˚C). Cell number as well as the viability was assessed using the Countess™ II Automated Cell Counter (Invitrogen). Directly after tissue dissociation scRNA-seq (v3, 10x Genomics, Cat. N: PN-1000075, PN-1000074, PN-120262) libraries were generated according to the manual instructions with a targeted recovery of 6000 cells/nuclei per sample. Before sequencing, scRNA-seq libraries were quantified by qPCR using KAPA Library Quantification Kit  (Roche, Cat. N: 07960298001). The size distribution of the obtained libraries was assessed using Agilent 2100 Bioanalyzer.
GEO Accession
GSM4710394