GSM4211253: CSR_DNA methylation_seq_6; Rattus norvegicus; Bisulfite-Seq
The genomic DNA (gDNA) of the testes was isolated by a TIANamp Genomic DNA Kit (TianGen), according to the instructions of the manufacturer. One hundred nanograms of gDNA were digested with 30 U of MspI enzyme (Fermentas) in 20 μl reactions at 37℃ for 16 h. After purification, digested products were blunt ended, and dA was added, followed by methylated-adapter ligation. The obtained DNA fractions were size selected in 200-450 bp using AMPure XP beads (Beckman Coulter). Bisulfite conversion was conducted using an EZ DNA Methylation-Direct Kit (ZYMO), following the instructions of the manufacturer. The final libraries were generated by PCR amplification using a HiFi HotStart Uracil+ Kit (Kapa).
GEO Accession
GSM4211253