Pooled sample consisting of samples: Cyst 511 S1|Cyst 551 S1|Cyst 512 S1|Cyst 576 S1|Cyst 505 S1|Cyst 513 S1|Cyst 521 S1|Cyst 529 S1|Cyst 545 S1|Cyst 577 S1|Cyst 562 S1|Cyst 570 S1|Cyst 523 S1|Cyst 539 S1|Cyst 547 S1|Cyst 555 S1|Cyst 556 S1
Primers were used to amplify DNA in 25-uL multiplex polymerase chain reactions (PCRs) as described previously 15.  For each sample, three multiplex PCRs were performed, with each multiplex PCR containing 22 to 50 primer pairs.  Reactions were purified with AMPure XP beads (Beckman Coulter) and eluted in 100 uL of Buffer EB (Qiagen, cat. no. 19086).  0.25% of the purified PCR products were then amplified in a second round of PCR, as described in Springer et al.15.  The PCR products were purified with AMPure and used for sequencing on a MiSeq or HiSeq instrument