Ribosome profiling of Mus Musculus: adult female B cells
Cells treated with or without GC7 were lysed and spiked with yeast lysate. The lysates were RNase I-digested, ribosome purified and miRNA was extracted. The miRNA was ligated with the linker (AppCTGTAGGCACCATCAAT/3ddC), reverse transcribed with the RT Oligo (ATCTCGTATGCCGTCTTCTGCTTG) and rRNA-depleted. Semi-quantitative PCR was used for amplification of the library.