laminectomy at 7 days post-injury
TRIzol Reagent (Invitrogen, Cat. No. 15596018) was used to isolate total RNA from the collected samples from laminectomy-operated sites at 7 dpi according to the manufacturers protocol. Qubit assay (ThermoFisher Scientific, Cat No: Q33140) was used to quantify the isolated RNAs. Gel electrophoresis (1% agarose) was used to assess their quality. mRNA sequencing libraries were prepared using TrueSeq stranded mRNA (Illumina, Cat no: 20020594) kit following the producers instructions. RNA sequencing was performed on Illumina NextSeq 500 platform using NextSeq 500/550 High Output Kit v2.5 150 cycles (Illumina, Cat no: 20024907).