GSM2445233: WT day 10 pre3; Drosophila melanogaster; RNA-Seq
Male flies were harvested at indicated time point and heads were removed by freezing the flies in liquid nitrogen followed by five rounds of vortexing and freezing. RNA was extracted from isolated GFP-tagged photoreceptor nuclei using previously described method by Ma J. and Weake V.M., J Vis Exp 2014 with minor modifications: nuclei were washed with PBS (supplemented to final salt concentration of 300 mM NaCl) following affinity purification. Libraries were prepared using NuGen Ovation Drosophila RNA seq system. rRNA was depleted using InDA-C technology.
GEO Accession
GSM2445233