GSM1181853: Runx3_CD4DC_CHIP_Sample_input1; Mus musculus; ChIP-Seq
Isolated cells were fixed in 1% formaldehyde and sonicated to yield DNA fragments of ~300bp. For immunoprecipitation, 40ul of anti-Runx3 Ab were added to 15 mL of diluted, fragmented chromatin; whole cell extract fragmented chromatin served as control. DNA was purified using QIAquick spin columns (QIAGEN). For ChIP-seq analysis, indexed CiHP-seq libraries were prepared as published Blecher-Gonen et al. Nature Protocols 8, 539–554 (2013).  Illumina sequencing of short reads (50 bp) was performed in one lane of Hiseq 2000 using v3 clustering and sequencing reagents.
GEO Accession
GSM1181853