GSM3206938: autocatalytic_circGFP_rep1; Homo sapiens; OTHER
Total RNA was isolated from HeLa cells using Qiagen RNeasy columns. RNA was ribo-/polyA- selected and treated with RNase R to remove linear RNA. Circular RNA was then fragmented to ~30-60 nts, and m6A residues were isolated by crosslinking anti-m6A antibody to fragmented RNA. RNA ligation for library construction was performed on affinity beads and RNA was subsequently transformed into dsRNA libraries using RT, cDNA circularization, and PCR amplification.
GEO Accession
GSM3206938