D.koepferae-D. buzzatii BC1 hybrid ovaries small RNA population
Small RNA was purified from 70 pairs of D. koepferae-D. buzzatii BC1 hybrid ovaries, following the manual small RNA purifying protocol described by Grentzinger et al. (2013), which significantly reduces endogenous contamination and degradation products abundance. After small RNA isolation, samples were gel-purified and precipitated. Sequencing was performed on an Illumina Hiseq 2500 platform by FASTERIS SA (Switzerland).