WXS of Homo sapiens
Genomic DNA was extracted from peripheral blood or tissues using FastPure Cell/Tissue DNA Isolation Mini Kit (Vazyme corp., cat: DC102). The genomic DNA was then fragmented to generate 300-500bp by Q800R Sonicator. The pairedend libraries were prepared following the protocol. NimbleGen SeqCap probes (Roche NimbleGen, Madison, Wis) were used for in-solution hybridization to enrich target sequences. Enriched DNA sample were sequenced on a NextSeq500 sequencer