16S-rRNA Sequencing: Healthy male induced sputum
Amplicon libraries were prepared using Nextera XT Index Kit; Universal primers were used to amplify V3-V4 hypervariable region; Illumina adaptors i5 and i7 and common adapters P5 and P7 were used; Purification of amplicon libraries by 1X Ampure XP beads and checked on Agilent High Sensitivity (HS) chip on Bioanalyzer 2100 and quantified on fluorometer by Quibit dsDNA HS Assay kit.