RNA-seq Caenorhabditis elegans: whole animal
Age synchronized day 1 adult were harvested for RNA extraction. Total RNA was extracted.Sample lysis was  performed using a Tissuelyser and a Trizol-chloroform based method was used  in conjunction with the Zymo RNA Clean & Concentrator kit to obtain  RNA. The Illumina TruSeq stranded mRNA  kit was used to obtain stranded mRNA via Oligo-dT bead capture, and cDNA  libraries were prepared from 500ng RNA per sample. Each sample was  multiplexed on 6 lanes of the Illumina HiSeq 4000 sequencer, generating  2x150bp paired end reads, with about 43 to 73 million reads per sample. The  reads were trimmed of adapters contamination and we removed 20 bp from the 5  and 3 ends using TrimGalore Version0.6.0. Only reads with a base quality of  >Q25 were maintained.