GSM4711439: Macrophages LPS Treated Rep 1; Mus musculus; RNA-Seq
C57BL/6 mice were purchased from Charles River Laboratories (Malvern, PA) and acclimated for one week at 22 ± 2°C on a light/dark cycle of 12 h. Animals were housed two per cage and allowed access to food and water ad libitum. Experiments were performed using the approved protocol by the Rutgers University Institutional Animal Care and Use Committee. All in vivo experiments were standardized to MIC-1 (based on 38.9% w/w MIC-1 in MSE). C57BL/6 mice divided into three groups of 6 animals randomly as follows: Group a: Vehicle Control group injected with saline; Group b: LPS (10 mg/kg); treated group c: MIC-1 (80mg/kg) gavaged group injected with LPS. Mice were pretreated (3 days) with MIC-1 in 10% DMSO (Sigma-Aldrich) via oral gavage before an LPS intraperitoneal injection and then sacrificed at 16 hours for tissue and blood collection. RNA was extracted with the RNeasy Mini Kit (Qiagen) according to the manufacturer's protocol.
GEO Accession
GSM4711439