GSM3891268: ATACseq_E76K1; Homo sapiens; ATAC-seq
ATAC-seq was performed according to the protocol in Buenrostro et al. (2015). 50,000 cells were suspended in cold lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% NP40). Crude nuclei were isolated by centrifugation at 500 g for 10 min and resuspended in 25 ml of TD Buffer (2x reaction buffer, Illumina). Nuclei pellet was collected (500 g, 5 min) and suspended in transposition reaction mix (25 ml, 2.5 ml TDE1, 22.5 ml l H2O). The reactions were incubated at 37ºC for 30 min with gentle mixing. DNA was immediately purified using Qiagen MinElute PCR Purification kit. Libraries were PCR amplified according to protocol and purified using Qiagen MinElute PCR Purification kit. Double sided bead purification (0.5x beads volume, followed by 1.3x beads volume) was performed using AMPure XP beads to enrich for fragments between 150 bp to 1000 bp. Library quality and fragment sizes were accessed using Bioanalyzer (Agilent). ATAC-seq libraries were sequenced with 150 bp paired end sequencing on HiSeq X (Illumina).
GEO Accession
GSM3891268