Illumina HiSeq 2500 sequencing; RNA-seq analysis of branchial arches and outflow tract of the mouse embryo at E10.5 and E11.5
RNA-seq analysis of branchial arches and outflow tract of the mouse embryo at E10.5 and E11.5
CD1 mice were time-mated to obtain branchial arches and OFT. Branchial arches and OFT were dissected and snap frozen in dry ice.  RNA was extracted using Trizol (Invitrogen, 15596-018), pooling 3-5 pairs of BAs for each sample. RNA-seq was performed in duplicates.  Library preparation of samples was performed using the Illumina TruSeq Stranded mRNA Sample Preparation Kits (Illumina)
Experimental Factor: embryonic day 10.5
developmental stage