GSM3890959: A_M0; Homo sapiens; RNA-Seq
Macrophage RNA was isolated using the Aurum Total RNA Mini Kit (Bio-Rad Laboratories, Hercules, Ca), quantified using the Nanodrop 1000 Spectrophotometer (Thermo Fisher), and 0.5 g of RNA converted to cDNA using an iScript kit (Bio-Rad Laboratories Canada, Mississauga, ON, Canada). RNA sequencing was performed at the Centre for Health Genomics and Informatics, Univ. Calgary, where samples were made into libraries using the Illumina TruSeq Stranded mRNA LT library preparation kit