Transcriptome analysis in mandarin fish big size group
To identify the key genes involved in individual difference in body size of mandarin fish, cDNA libraries were constructed from small-size group (S30) and big-size group (B30) at 30 dph, small-size group (S180) and big-size group (B180) at 180 dph. All fish in the four groups were cultured under the same environment. Brain and liver tissues were immediately frozen in liquid nitrogen for RNA isolation. After confirming RNA integrity, equal amount of total RNA from 6 fish in each group were pooled to construct the cDNA libraries for digital gene expression profiling and transcriptome analyses.