Pooled sample consisting of samples: Cyst 305 S1|Cyst 307 S1|Cyst 309 S1|Cyst 310 S1|Cyst 311 S1|Cyst 313 S1|Cyst 318 S1|Cyst 322 S1|Cyst 323 S1|Cyst 325 S1|Cyst 330 S1|Cyst 337 S1|Cyst 361 S1|Cyst 365 S1|Cyst 366 S1|Cyst 368 S1|Cyst 375 S1|Cyst 379 S1|Cyst 380 S1
Primers were used to amplify DNA in 25-uL multiplex polymerase chain reactions (PCRs) as described previously 15.  For each sample, three multiplex PCRs were performed, with each multiplex PCR containing 22 to 50 primer pairs.  Reactions were purified with AMPure XP beads (Beckman Coulter) and eluted in 100 uL of Buffer EB (Qiagen, cat. no. 19086).  0.25% of the purified PCR products were then amplified in a second round of PCR, as described in Springer et al.15.  The PCR products were purified with AMPure and used for sequencing on a MiSeq or HiSeq instrument