GSM3043114: Lrp::kanR Minimal+LIV transition point rep2-ChIPseq input; Escherichia coli; ChIP-Seq
RNA was harvested using Qiagen RNAProtect. RNA was isolated, and rRNA depletion was performed according to the Illumina Ribo-Zero rRNA Removal Kit for Bacteria. Specific Lrp-DNA complexes were isolated using a monoclonal antibody developed against Lrp, and DNA was isolated by phenol-chloroform extraction. DNA was preprared for sequencing using the NEBNext DNA Library Prep Kit.  RNA was prepared fro sequencing using the NEBNext Ultra Directionaly RNA Library Prep Kit.
GEO Accession
GSM3043114