GATA2 ChIP analysis of K562 cells
"We generated a monoclonal antibody against the antigen (residues 192-245) of human GATA2, and obtained antibodies against H3K4me3 (Abcam, ab8580). The GATA2-mediated immunoprecipitated DNA and non-immunoprecipitated whole genome control (input DNA) were used to prepare libraries for deep sequencing and analysed using massively parallel sequencing. ChIP-DNA or INPUT control was prepared for sequencing according to the Illumina/Solexa Genomic DNA protocol. Fragmented DNA was ligated with Solexa linkers. A 1:100 dilution of the Adaptor Oligo Mix (Illumina) was used in the ligation step. Subsequently, PCR was performed with 18 cycles, and samples were subjected by 2% agarose gel electrophoresis. A narrow range of fragment sizes, between 175-225 bp, was excised and the containing DNA was eluted."