GSM4648202: RNASeq_Ubc9-WT_replicate2; Mus musculus; RNA-Seq
Total RNA was extracted from cells using Trizol (Ambion Life Technologies) and chloroform, followed by washes with isopropanol and 70% ethanol. 1 mg of extracted RNA was used as an input for mRNA isolation using PolyA beads. For H1-TKO RNA-seq: Libraries preparation was carried out using Lexogen's SENSETM mRNA-Seq Kit V2 according to manufacturer's instruction. For Ubc9-depletion RNA-seq: Libraries preparation was carried out using ScriptSeq v2 RNA-seq library preparation kit according to manufacturer's instruction.
GEO Accession
GSM4648202