Pooled sample consisting of samples: Cyst 209 S1|Cyst 210 S1|Cyst 218 S1|Cyst 219 S1|Cyst 221 S1|Cyst 222 S1|Cyst 230 S1|Cyst 232 S1|Cyst 239 S1|Cyst 251 S1|Cyst 258 S1|Cyst 263 S1|Cyst 266 S1|Cyst 269 S1|Cyst 274 S1|Cyst 275 S1|Cyst 277 S1|Cyst 286 S1|Cyst 288 S1|Cyst 292 S1|Cyst 294 S1
Primers were used to amplify DNA in 25-uL multiplex polymerase chain reactions (PCRs) as described previously 15.  For each sample, three multiplex PCRs were performed, with each multiplex PCR containing 22 to 50 primer pairs.  Reactions were purified with AMPure XP beads (Beckman Coulter) and eluted in 100 uL of Buffer EB (Qiagen, cat. no. 19086).  0.25% of the purified PCR products were then amplified in a second round of PCR, as described in Springer et al.15.  The PCR products were purified with AMPure and used for sequencing on a MiSeq or HiSeq instrument