GSM2443540: mESC_Rsf1_ubH2Adel_ChIP-seq_rep1; Mus musculus; ChIP-Seq
Cells were cross-linked with 1% formaldehyde. Chromatin was disrupted into 100-250 bp fragments by sonication and precipitated using antibody and protein A beads as indicated in the text and protein A beads. After elution from the beads and reversing the cross-linking, the DNA fragments in immunoprecipitates were extracted with phenol/chloroform and recovered by ethanol precipitation. Immunoprecipitated DNA was ligated with 3’- and 5’-adapters for amplification and sequencing by using Illumina II.
GEO Accession
GSM2443540