Batch CRISPRi screen of homo sapiens: HeLa cells treated with H2O2
Genomic DNA was extracted from HeLa cells treated with multiple H2O2 doses containing the CRISPRi sgRNA H2O2 batch library using the Qiagen Blood Maxi Kit. Nested PCR was used to amplify the sgRNA cassette and attach Illumina adapters. Libraries were purified, quantified, multiplexed and sequenced on an Illumina NextSeq 500.