Cgmed15B-pH 6.0
Studying for tolerance of Cgmed15B. Samples were collected in the YNB at pH 6.0 culture 6 h. And total RNA were extracted using Mini BEST Universal RNA Extraction Kit. For pure sequencing data, rRNA was filted from total RNA using the NEBNext poly(A) mRNA magnetic isolation module. And then Illuminiar Paired-END library was constructed, and appropriate insert size was seleted. The sequencing data will be analysed with before RT-PCR result and published genomes.