WGS of Nocardia farcinica:cystic fibrosis patients´sputum
Paired-end libraries (Nextera-XT DNA Library Preparation Kit, Illumina 1.9), were adapted and sequenced at 2x150 using the Illumina NextSeq500 platform. After quality control analysis involving fastQC v.0.11.3 (http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) and Trimmomatric v.0.36 software, 3.6-3.1 million paired-end reads of 50-151bp were retrieved. Read assembly was performed using SPAdes v.3.8.0 software (with kmer=77) and the quality evaluated using QUAST software.