small RNA-seq of Arabidopsis thaliana: stems 4cm above a Cuscuta campestris haustorium
Total RNA was isolated using Tri-Reagent (Sigma) and used as input to the NEBNext Small RNA Library Prep Set for Illumina. Bar coded Small RNA-seq libraries were made following the manufacturer's instructions, quality-controlled, and pooled for single-end, 50nt sequencing on an Illumina HiSeq2500 instrument in rapid run mode. Libraries were deconvoluted by barcodes.