GSM4251755: Hi-C seq; Saccharomyces cerevisiae; Hi-C
Snap freezed cells were thawed, processed with the Hi-C protocol and the resulting DNA was extracted using phenol-chloroform extraction methods before sequencing Biotinylated fragments were enriched using streptavidin magnetic beads (C1), DNA ends were A-tailed and NextFlex (Bioo Scientific) barcoded adapters were ligated while the DNA was on the beads. Resulting libraries were minimally amplified by PCR and sequenced using paired end 75 bp reads on a NextSeq550 (Illumina; Brighton Genomics).
GEO Accession
GSM4251755