GSM1834793: Control_root; Phoenix dactylifera; ncRNA-Seq
Leaf and root tissues from 6 control and salt-treated seedlings were separately pooled, and small RNAs were isolated using the miRNA Mini Kit (Qiagen), following the manufacturer’s instructions. The quality and quantity of the RNA samples were tested using an Agilent 2100 Bioanalyzer (Agilent). The small RNAs were ligated with 3’and 5’adaptors, and RT-PCR was performed using the TruSeqTM Small RNA kit (Illumina) according to the manufacturer’s instructions. The expected final PCR product was isolated from the gel and purified and sequenced using Illumina Hiseq2000 sequencing technology (Illumina).
GEO Accession
GSM1834793