Whole genome bisulfite sequencing  of xenograft from human GIST sample S1: Sample S1_xenograft_WGBS
"Total genomic DNA was isolated using the DNAeasy Blood & Tissue Kit (Qiagen) and sheared using the Covaris LE220. Ampure XP beads (Agencourt) were used to size select gDNA fragments within 150-320 bp and sheared distribution was verified via BioAnalyzer (Agilient). One microgram of gDNA was end repaired, 3' A base tailed (KAPA Hyper Prep Kit no. KK8502) and ligated to sequencing adaptor (Roche SeqCap Epi Enrichment System). Ligated products were purified using Ampure XP beads. Following bead clean-up, products were bisulfite-converted using the EZ DNA Methylation-Lightning Kit (Zymo Research) and then PCR amplified using KAPA HiFi U+ HotStart ReadyMix (KAPA no. KK2800)."