targeted long read RNA seq of human HYDIN2 in fetal brain run 1
Targeted RT-PCR was performed on pooled fetal brain RNA with primers designed to amplify full-length transcripts or segments of transcripts of the duplicated human gene HYDIN2. For amplicons entirely within the duplication, transcripts from both HYDIN and HYDIN 2 were amplified. Amplicons underwent standard PacBio library prep and were sequenced on the RS II using P5C3 chemistry.