H3K27ac ChIP-seq of human GIST sample KIT1: Sample KIT1_H3K27ac
"Minced frozen tissues were crosslinked in 1% formaldehyde and snap frozen in liquid nitrogen before storage at -80o C for at least overnight. Sonication of samples were calibrated such that DNA was sheared to between 300 and 700 bp fragment length. CTCF was precipitated with a monoclonal rabbit CTCF antibody, clone D31H2 (Cell Signaling no. 3418). Histone H3K27 acetylation was immunoprecipitated with antibody from Active Motif (no. 39133). ChIP DNA was used to generate sequencing libraries by end repair (End-It DNA repair kit, Epicentre), 3' A base overhang addition via Klenow fragment (NEB) and ligation of barcoded sequencing adapters. Barcoded fragments were amplified via PCR."
alignment_software
BWA 0.7.4