illumina sequencing of Paracoccus DNA, generation 119, N-limited
DNA was extracted from a pellet of 2 ml N-limited chemostat culture, generation 119, as described in Zhou et al (1996) after incubation for 30 min at 37 °C with 3 mg mL-1 lysozyme and 0.1 mg mL-1 RNase. 50ng of extracted DNA were used for barcoded illumina library preparation according to the Nextera(R) DNA Sample Preparation Kit manual.