GSM4211154: Monocytes from mouse wt 2; Mus musculus; RNA-Seq
Tumors were collected 21 days after tumor cell injection and washed once with physiologic solution. Single-cell suspension were obtained by mechanical and enzymatic dissociation in 0.1 mg/ml Type IV Collagenase for 1h at +37°C. RNA was purified from FACS sorted MN/MCA1 tumor-infiltrating monocytes (CD45+, CD11b+, Ly6G-, CD11c-, F4/80-, Ly6C+) and macrophages (CD45+, CD11b+, Ly6G-, CD11c-, Ly6C-, F4/80+) with the Single Cell RNA Purification Kit. Libraries for mRNA sequencing were prepared starting from 5 ng totRNA for each sample by using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech-Takara), this allows to add defined PCR adapters directly to both  ends of the first-strand cDNA.
GEO Accession
GSM4211154