Diurodrilus subterraneus transcriptome
This is an RNA-seq library constructed as part of a study on the phylogenetic relationships of Spiralia. Pooled adult animals were flash frozen and stored on dry ice or in -80 C freezers prior to RNA extraction, and poly-A RNA was extracted using the DYNAbeads DIRECT mRNA selection kit. A cDNA library was prepared from this sample using the IntegenX mRNA protocol on an Apollo324 robot, and sequenced in multiplex on an Illumina HiSeq 2500 rapid run.