Pooled sample consisting of samples: Cyst 710 S1|Cyst 716 S1|Cyst 722 S1|Cyst 739 S1|Cyst 740 S1|Cyst 753 S1|Cyst 754 S1|Cyst 757 S1|Cyst 761 S1|Cyst 765 S1|Cyst 769 S1|Cyst 776 S1|Cyst 782 S1|Cyst 783 S1|Cyst 790 S1
Primers were used to amplify DNA in 25-uL multiplex polymerase chain reactions (PCRs) as described previously 15.  For each sample, three multiplex PCRs were performed, with each multiplex PCR containing 22 to 50 primer pairs.  Reactions were purified with AMPure XP beads (Beckman Coulter) and eluted in 100 uL of Buffer EB (Qiagen, cat. no. 19086).  0.25% of the purified PCR products were then amplified in a second round of PCR, as described in Springer et al.15.  The PCR products were purified with AMPure and used for sequencing on a MiSeq or HiSeq instrument