GSM2445174: UCLA17 iMeLC induction replicate 2 RNA-seq; Homo sapiens; RNA-Seq
Sorted cells or cell pellets were lysed in 350uL of RLT buffer (QIAGEN) and total RNA was extracted with RNeasy micro kit (QIAGEN, 74004). Total RNA was reverse transcribed and cDNA was amplified using Ovation RNA-Seq System V2 (Nugen, 7102-32) according to manufacturer’s instructions. Amplified cDNA was fragmented into ~200bp by Covaris S220 Focused-ultrasonicators. RNA-seq libraries were generated using Ovation® Rapid Library Systems (Nugen, 0319-32 for index 1-8 and 0320-32 for index 9-16) and quantified by KAPA library quantification kit (Illumina, KK4824). Library type = Ovation RNASeq System V2
GEO Accession
GSM2445174