WGS of Mycoplasma cynos: ATCC 27544
The Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA) was used to prepare fragment libraries of the Mycoplasma cynos and Mycoplasma mucosicanis DNA for whole genome sequencing; this was performed according to the manufacturers instructions. Tagmented libraries were purified using AMPure XP Beads (Beckman Coulter Life Sciences, Indianapolis, IN) at a concentration of 0.5x. Barcoded  whole genome libraries were then pooled and run on Illumina MiSeq Reagent Kit (V3) cartridge at 2x250 cycles using a MiSeq next-generation sequencer.