GSM2966502: H3K4me3_1M-sonication-ChIP-ESC_rep2; Mus musculus; ChIP-Seq
Crosslinked cells were lysed and sonciated to 200-800 bp fragments. Soluble chromatin was used for ChIP experiments. DNA on beads were tagmented with Tn5 transposome following the Nextera protocol. After PCR enrichment using NEBNext Q5 Ultra II master mix, products were purified and size selected by AMPure XP beads for 200-1000 bp.
GEO Accession
GSM2966502