GSM4760984: VSMCs, si-circ-1; Mus musculus; RNA-Seq
VSMCs were washed twice in cold PBS and lysed in RNAiso (Takara, Cat# 108-95-2) before sent to BioWavelet (Chongqing, China) for sequencing. The first-strand of cDNA was synthesized using SuperScript III reverse transcriptase (Invitrogen, Cat# 18080-085) followed by the second-strand synthesis using synthesis enzyme mix (Biowavelet, Cat# BWR001) according to guidelines. RNA libraries were prepared using TruePrep DNA Library Prep Kit for Illumina (Vazyme, Cat# TD503) according to the standard protocol.
GEO Accession
GSM4760984