Spatial RNA-Seq of Lupinus albus Cluster Root  S2
Seeds of white lupin (Lupinus albus L. cv. Amiga, from Florimond Desprez, France) were germinated for 4 days on vermiculite soaked with water. Thereafter, the seedlings were transferred to an aerated nutrient solution in the absence of phosphate in growth chambers under controlled conditions (16h light/8h dark, 25C day/20C night, 65% relative humidity and PAR intensity 200 mol.m.s). After 12 days of culture, ten cluster roots coming from four independently grown plants were harvested and dissected in eight parts of 0.5-cm from the apex of the lateral root that carries the cluster root. As control, 1 cm of lateral roots without cluster roots, sampled 1 cm away from the primary root, were collected. Four biological replications were produced for each experiment.