RRBS of pediatric AML patients
Starting from high quality genomic DNA, extract from RMS tumour biopsies,  Reduced-representation bisulfite (RRBS) libraries were performed. The following steps were taken: restriction enyme digestion-3'A addition and adaptor ligation-size selection- bisulfite treatment -PCR amplification-library construction. At least the qualified RRBS library would be used for sequencing.