GSM2526582: Zs_pos_48hr_rep1; Mus musculus; RNA-Seq
Total RNA was extracted from sorted DP thymocytes using Trizol (Life Technologies). Adapters were ligated to the 5' ends of total RNA using a template-switch RACE protocol. Tcra transcripts were amplified, and Illumina adapter sequences added, using a sense primer that annealed to the 5' RACE adapter and an antisense primer that annealed to Trac.
GEO Accession
GSM2526582