Pooled sample consisting of samples: Cyst 159 S1|Cyst 161 S1|Cyst 162 S1|Cyst 163 S1|Cyst 164 S1|Cyst 165 S1|Cyst 167 S1|Cyst 168 S1|Cyst 169 S1|Cyst 170 S1|Cyst 171 S1|Cyst 172 S1|Cyst 173 S1|Cyst 174 S1|Cyst 175 S1|Cyst 176 S1|Cyst 177 S1
Primers were used to amplify DNA in 25-uL multiplex polymerase chain reactions (PCRs) as described previously 15.  For each sample, three multiplex PCRs were performed, with each multiplex PCR containing 22 to 50 primer pairs.  Reactions were purified with AMPure XP beads (Beckman Coulter) and eluted in 100 uL of Buffer EB (Qiagen, cat. no. 19086).  0.25% of the purified PCR products were then amplified in a second round of PCR, as described in Springer et al.15.  The PCR products were purified with AMPure and used for sequencing on a MiSeq or HiSeq instrument