GSM3781644: scRNA_seq_E13.5_skin; Mus musculus; RNA-Seq
All skin tissues were obtained from male fetuses prior to digestion. To prepare dorsal skin single cell suspension for single-cell RNA sequencing, the fetus back skin tissues were isolated via microdissection and 0.25% trypsin/EDTA solution was used to digest E13.5 and E16.5 fetus dorsal skin tissues at 37 °C for 5 min. For P0 mice dorsal skin tissues, 2 mg/ml collagenase IV (Sigma, St Louis, MO, USA, Cat#C5138) was used to digest skin tissues at 37 °C for 30 min. After trypsinization, the skin tissues were mechanically dissociated into single cell suspension by pipetting, the cell suspensions were then filtered through a 40 μm nylon cell strainer (BD Falcon, BD Biosciences, San Jose, CA, USA, Cat#352340) prior to single cell library construction. The single-cell Gel-Bead in Emulsions (GEMs) were then generated with Chromium 10x Genomics single-cell system by using single-cell 3' Library and Gel Bead Kit V2 (10x Genomics Inc., Pleasanton, CA, USA, Cat#120237). Captured cells were then lysed and the released RNA were barcoded through reverse transcription in individual GEMs. Single-cell RNA-seq libraries were prepared using Single Cell 3' Library Gel Bead Kit V2 (10x Genomics Inc., Pleasanton, CA, USA, Cat#120237) following the manufacture's introduction.
GEO Accession
GSM3781644