GSM4251977: ovarian organoids_scRNA-seq; Mus musculus; RNA-Seq
Retinas were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Double strand cDNA was firstly generated from Microbeads-captured single cell transcriptome following several steps including reverse transcription, second strand synthesis, end preparation, adapter ligation and whole transcriptome amplification. Then final cDNA library was generated from double strand full length cDNA by random priming amplification with BD Rhapsody cDNA Kit (BD Biosciences, 633773) and BD Rhapsody Targeted mRNA & AbSeq Amplification Kit (BD Biosciences, 633774). Library was sequenced in a PE150 mode (Pair-End for 150bp read) on the TenX instrument (Illumina).
GEO Accession
GSM4251977