GSM3044555: SRSF2 WT RNA-seq rep 1; Homo sapiens; RNA-Seq
HITS-CLIP: cells were UV-crosslinked (400mJ, Stratalinker 2400, Stratagene). RNA–protein complexes were immunoprecipitated with anti-Flag M2 Agarose beads (Sigma). RNA was partially digested with RNase A (Affymetrix) and P32-labeled (Perkin Elmer). SRSF2-RNA complexes were isolated by SDS-PAGE, treated with proteinase K, followed by RNA linker ligation.   RNA_seq: QIAGEN RNeasy minikit protocol HITS-CLIP: Ilumina small RNA library protocol for linker ligation.   RNA-seq: rRNA depletion
GEO Accession
GSM3044555