GSM3780795: 166_NT_rep2; Acinetobacter baumannii; RNA-Seq
The total RNA of each sample was extracted by miRNeasy Mini Kit (QIAGEN, Germany) following the manufacturer's instructions. Genomic DNA residue was removed by TURBO DNA-freeTM Kit TURBOTM DNase Treatment and Removal Reagents (Ambion Inc., USA). The resulting gDNA-free total RNA samples were immediately processed for rRNA depletion. rRNA was depleted by using the Ribo-Zero Magnetic kit (bacteria) (Illumina, Inc., USA), following the manufacturer's instructions. For each sample, 500 ng of rRNA depleted total RNA was used to generate double stranded cDNA library through TruSeq® Stranded Total RNA Sample Preparation kit (Illumina, Inc., USA). The samples were then sequenced through 100 bp paired end (PE) sequencing on Illumina HiSeq2500 platform. Nearly 75 million unique 101 bp reads were obtained from each of the 6 independent A. baumannii 6772166 cultures, representing more than 310-fold genome average coverage (A. baumannii 6772166 chromosome and plasmid size together is around 4 Mb).
GEO Accession
GSM3780795