Whole transcriptome analysis of Sox17.Epi and Endo cells in mouse embryos
Whole transcriptome analysis of Sox17.Epi and Endo cells in mouse embryos
Total RNA was amplified using the Ovation RNA-seq system (Nugen, 7100-08). The library was constructed using the Encore NGS Multiplex System I (Cat. # 301-32). The transcriptional sequencing methods were performed essentially as previously described (Mortazavi 2008), which is a modification of the standard Illumina methods (Bentley 2008).
Experimental Factor: cell type
Sox17 expressing cell in ventral pancreatic bud