GSM4710735: SHR cardiac fibroblast-rep5; Rattus norvegicus; RNA-Seq
Single cell suspension was then processed by the Chromium Controller (10x Genomics) using single Cell 3' Reagent Kit v3 (Chromium Single Cell 3' Library & Gel Bead Kit v3, catalog number: 1000075; Chromium Single Cell B Chip Kit, 48 runs, catalog number: 1000073; 10x Genomics). Two separate cell counts on a hemocytometer were used to load ~4000 cells/sample for 10X GEM creation. Any remaining cell suspension after GEM creation was stained with NucBlue Live Cell Stain (ref: R37605; Invitrogen | Thermo Fisher Scientific) and NucGreen Dead 488 (ref: R37109; Invitrogen | Thermo Fisher Scientific). After a 20-minute incubation, stained cells were again counted using a Countess™ II Automated Cell Counter (ref: A27977; Invitrogen | Thermo Fisher Scientific). Single cell sample libraries were generated using 10X 3' V3 regents following 10X's 3' V3 instructions. After library generation, the single sample libraries were pooled equimolar and sequenced using HiSeq4000 (Illumina) and V4 chemistry (HiSeq PE Cluster Kit v4 cBot, catalog number: PE-401-4001; HiSeq SBS Kit V4 250 cycles, catalog number: FC-401-4003; Illumina) with 254 bp paired end reads.
GEO Accession
GSM4710735