Mpig6b mutant mice replicate #3
Whole Exome Sequencing of mouse genomic DNA (50 ng input) was performed according to Agilents protocol for the SureSelect QXT reagent kit and SureSelect XT Mouse All Exon plus capture probes for Illumina paired-end sequencing (49.6 Mb capture spanning 221,784 exons and 24,306 genes). Sequencing was performed with SureSelect QXT sequencing primer in 2x75bp paired-end mode on the NextSeq 500 platform (Illumina) with the High Output Kit v2.5 (150 Cycles). Demultiplexed FASTQ files were generated with bcl2fastq2 (Illumina).