Raw Transcriptome NGS Illumina HiSeq2000 for Folsomia candida
1.5 *g of normalized cDNA in 100*l 10mM Tris/HCl was fragmented. After fragmentation, end repair, ligation of adapters, and enrichment of the fragments was done using the Paired-End Sequencing kit of Illimina*, according to the manufacturer’s protocol. All purifying steps were done using the MinElute PCR purification kit (Qiagen*). Quality and concentration of the fragmented and enriched library was verified on a lab-on-a-chip (Agilent Technologies). Next-generation sequencing of the 180 – 200 bp fragments for F. candida was performed on Illumina HiSeq 2000 platform.