GSM4762586: 85_percent_d14_Hsap_Mmul_Chimera; Homo sapiens; Mus musculus; RNA-Seq
Total RNA was then purified from 350 µL RLT-Plus Buffer using RNeasy Plus 96 and Micro Kits (Qiagen, Netherlands) and quantitated with the Quant-iT RNA Assay Kit (Thermo Fisher Scientific, USA).  RNA was diluted to one hundred nanograms for input. The Ligation-Mediated Sequencing (LM-Seq) protocol was used to prepare and index all cDNA libraries (Hou et al 2015). Final cDNA libraries were quantitated with the Quant-iT PicoGreen Assay Kit (Thermofisher, Waltham, Massachusetts). Twenty-five to forty-eight uniquely indexed samples were pooled per lane on an Illumina HiSeq 2500 with a single 51 base pair read and a 10 base pair index read.
GEO Accession
GSM4762586