16S amplicon of soil metagenome: extracted soil community cultivated in droplets, replicate 2
extracted soil community encapsulated in droplets, droplets incubated for 1 month, emulsion breaking and collection of cell biomass, mechanical cell lysis by bead beating, phenol DNA extraction, DNA precipitation with ethanol, Q-Sepharose purification for removal of humic acids, PCR of V3-V4 region with 314F and 758R primers, amplicon purification and quantification, pooling, further library prep as in standard illumina instructions