GSM1176146: MBnolB75; Clostridium acetobutylicum; RNA-Seq
Samples for RNA isolation were collected by centrifugation at 5000 rpm at 4°C for 10 min and the pellets were stored at -80°C.  RNA isolation was carried out using the Qiagen’s miRNeasy Mini kit (Jones et al, 2008). After RNA extraction, mRNA and sRNA were enriched by using Microbe Express kit from Ambion ® kit as per the manufacturer’s protocol. The Ovation Prokaryotic RNA-Seq System (NuGEN® Technologies, Inc, San Carlos, CA) was used to synthesize cDNA from 500 ng of enriched RNA. 50 μL of cDNA was used to construct libraries using the TruSeq DNA Sample preparation kit .
GEO Accession
GSM1176146