RNA-seq of control petal
Total RNA samples were isolated from petal AZs of RhERF1-silenced plants and TRV control plants. Six RNA-seq libraries were prepared and sequenced using HiSeq 2000 system. RNA-seq data were processed, assembled, and annotated as previously described. RNA-seq reads were examined to remove low-quality (Q value <20) reads and adapters using Trmmomatic. High-quality clean reads were assembled de novo into contigs by Trinity program . To remove the redundancy of Trinity-assembled contigs, the contigs were further assembled de novo using iAssembler.