Pooled sample consisting of samples: Cyst 208 S1|Cyst 213 S1|Cyst 216 S1|Cyst 225 S1|Cyst 227 S1|Cyst 229 S1|Cyst 231 S1|Cyst 234 S1|Cyst 236 S1|Cyst 237 S1|Cyst 241 S1|Cyst 242 S1|Cyst 253 S1|Cyst 259 S1|Cyst 264 S1|Cyst 270 S1|Cyst 272 S1|Cyst 278 S1|Cyst 284 S1|Cyst 289 S1|Cyst 293 S1
Primers were used to amplify DNA in 25-uL multiplex polymerase chain reactions (PCRs) as described previously 15.  For each sample, three multiplex PCRs were performed, with each multiplex PCR containing 22 to 50 primer pairs.  Reactions were purified with AMPure XP beads (Beckman Coulter) and eluted in 100 uL of Buffer EB (Qiagen, cat. no. 19086).  0.25% of the purified PCR products were then amplified in a second round of PCR, as described in Springer et al.15.  The PCR products were purified with AMPure and used for sequencing on a MiSeq or HiSeq instrument