ITS Metagenome: Sordic and Non Sordic Endophyte and Rhizisphere
The primer pair ITS 1 and ITS  was used for the amplification of the ITS rRNA gene region.Amplicon products from each sample were pooled together in equal concentrations and were purified using Agencourt Ampure XP beads. The PCR product was then used to prepare a DNA library following the Illumina TruSeq DNA library preparation protocol. Forward and reverse reads were paired by sequencing service provider.