RNAseqofOryziasMelastigma:male liver
We chose fish from the group treated with 1/32 96-LC50 Cr(VI) (2.61mg/L) for 96h and control group as materials. The total RNA of the livers from three replicates was extracted by Trizol. Each replicate contained three fishes. The quality of total RNA was further determined with an Agilent 2100 Bioanalyzer, the concentrations were > 200ng / L. 260/280 ratios were > 1.8, the rRNA Ratio [28s/18s] > 0.9, and RIN > 5, which correspond with the sample quality requirements of total RNA for Illumina sequencing.