Sequencing  of testicular mice transcriptome in response to non-canonical estrogen receptor activity modulation
For mRNA sequencing, the libraries were generated with Illumina TruSeq Stranded mRNA Library Prep Kit. cDNA libraries were sequenced on a HiSeq4000 (Illumina, San Diego, CA, USA) with the following parameters: PE150 (150bp paired-end) and minimum 40 millions of raw reads (40M) which gave a minimum of 12Gb of raw data per each sample.