ATAT-seq of mus musculus: adult male spleen
CD8+ CD44hi T cells isolated from spleen of mice infected with LCMV clone13. The unfixed nuclei of these cells were tagged using tn5 transposase (TruePrepTM DNA Library Prep Kit V2 for Illumina) for 30 min at 37 C, and the resulting library fragments were generated by 10-12 PCR cycles, and finally sequenced on an Illumina HiseqXten-PE150.