transcriptom sequencing of BY-2 cells
Transcriptome sequencing and de novo assembly Solexa sequencing was performed as a commercial service in Beijing Genomic Institute. The mRNA was isolated from total RNA using beads coated with Oligo(dT) and cleaved into small fragments with divalent cations at elevated temperature. The fragments were used to synthesize first-strand cDNA using random hexamer-primers. The second-strand cDNA was synthesized and purified. These cDNA fragments then went through an end repair process and were ligated to sequencing adapters. The final products were purified and enriched by PCR to create the final cDNA library and subsequently sequenced by using Illumina HiSeq:trademark: 2000.