GSM3044546: Clarke_TCRseq_Beta_Donor-8_Tumor_CD8+_CD103neg; Homo sapiens; OTHER
TCR-seq was performed as described previously by Shugay et al., 2014  with 1ng of total RNA. Total RNA was purified using a miRNAeasy kit (Qiagen) and quantified as described previously (Seumois et al.Am J Clin Exp Immunol. 2012; PMID:23304658). TCR-seq was performed as described previously by Shugay et al., 2014  with 1ng of total RNA. DNA sequencing - libraries were prepared using True-seq nano DNA library kit (Illumina). Libraries were pooled and sequenced on the MiSeq Illumina platform
GEO Accession
GSM3044546