Amplicon of Osedax  deceptionensis microbiome: 16Palps
Amplification of V3-V4 hyper-variable  region of the 16S rRNA gene was done in one-step PCR using primers 341F  (Muyzer et al. 1993)- 806R (Caporaso et al. 2011). PCR products were  normalised and cleaned with the "SequalPrep Normalization Plate Kit"  (Invitrogen). Sequening was preformed at the Institute of Clinical Molecular  Biology (IKMB), University Kiel, Germany.