SL2 specific pulldown of poly-adenylated mRNA-seq from mixed stage worm sample
The remaining mRNA was split into two equal portions, and these were subjected to pull-downs using either the SL1-specific or the  SL2-specific biotinylated probe respectively. This resulted in two pools of mRNA highly enriched for the SL1-spliced and SL2-spliced transcripts respectively, which were then used for RNA-Seq experiments. The biotinylated probes used for enrichment of SL1 and SL2 transcripts corresponds to the variants Pp-SL1a and Pp_SL2b which have been reported to be observed most frequently on trans-spliced transcripts in P. pacificus (97% and 62% respectively, Guiliano and Blaxter 2006).