The RNA-seq data of OXLAC panicles at booting stage
The young panicles(panicles before heading) of wild-type, OXmiR397b and OXLAC plants (n = 20 plants for each sample) were collected to extract the total RNA.Briefly, mRNA was enriched by using the oligo(dT) magnetic beads and interrupted to short fragments (about 200 bp), then the first and second strand cDNA is synthesized using the mRNA fragments as templates. The double-stranded cDNA is purified and a single nucleotide A (adenine) addition is made. Finally, sequencing adaptors are ligated to the fragments. The required fragments are purified by agarose gel electrophoresis and enriched by PCR amplification. The library products are ready for sequencing analysis via Illumina HiSeq 2000.