Global transcriptomic profiling in barramundi Lates calcarcifer from rivers impacted by differing agricultural land-uses
Total RNA was extracted from the liver of wild caught fish.  Libraries were prepared using the TruSeq Stranded mRNA Sample Prep Kit (Illumina), using 1 µg of total RNA as input. The libraries were enriched using 13 cycles of PCR and the insert size ranged from 80-330 base pairs, with a median at 150 bp. Fourteen libraries were multiplexed in one lane of the HiSeq2500 and sequenced using TruSeq v4 SBS reagents and 125 bp paired-end reads.