Female Schistosoma japonicum
DNA was extracted from 33 pooled females. The worms were lysed using the Tissue Lyser II kit (QIAGEN) and DNA was isolated using the DNeasy Blood & Tissue Kit (QIAGEN). DNA was then sheared with a AFA  with Covaris Focused-ultrasonicator. Library preparation and sequencing (HiSeq 2500 v4 Illumina, 125bp paired-end reads) were performed at the Vienna Biocenter Next Generation sequencing facility (Austria).