Renseq analysis of wheat cultivar Gabo which carries the endogenous Sr50 gene
RNA was extracted from wheat seedlings at the fourth leaf stage and cDNA produced using a SuperScript III Reverse Transcriptase kit (Invitrogen, USA). DNA adapters were added to cDNAs and NLR sequences enriched using RenSeq exome capture with Tv2 baits and 125PE sequencing performed in HiSeq2500 platform by Arbor Biosciences (Ann Abor, USA).