Transcriptom of Populus euphratica
Illumina Hiseq 2000 library was constructed for Solexa sequencing. Poly (A) mRNA was first enriched with oligo (dT), and then was fragmented into small pieces of 200-700 bp using divalent cations at an elevated temperature. Based on these cleaved RNA fragments, we used random hexamer-primer and reverse transcriptase (Invitrogen) to synthesize cDNA. Three paired-end cDNA libraries with an insert size of 200 bp were constructed, and then sequenced using Illumina HiSeq™ 2000 to generate an average read length of 90 bp as raw data.