GSM1468960: no-tag Myc for Rpc25_Brf1 ChIP-seq; Schizosaccharomyces pombe; ChIP-Seq
Fission yeast cells grown at 30°C in YEA were shifted to 18°C for 5 min before 30 min fixation in 3% paraformaldehyde. Soluble chromatin fractions prepared from fixed cells were sonicated for 30 min by a Bioruptor (Diagenode). Tagged proteins were purified by each antibody and protein G-coupled Dynabeads (Life Technologies). Libraries were prepared from 5 ng of DNA using the TruSeq DNA sample prep kit (Illumina) for Myc samples or using the NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina® with the NEBNext® Multiplex Oligos for Illumina® (index Primers Set 1) for FLAG samples, following manufacturer's protocol. Sequencing was performed by an Illumina Genome Analyzer II (Myc) or an Illumina HiSeq 2000 (FLAG).
GEO Accession
GSM1468960