cDNA Sediment
We performed amplification of the 16S rRNA using 515F 5'-GTGNCAGCMGCCGCGGTAA-3' and 926R 5'-CCGYCAATTYMTTTRAGTTT-3' primers targeting the V4-V5 region. Each primer contained either the 454 Titanium A (5'-CGTATCGCCTCCCTCGCGCCATCAG-3') or B (5'-CTATGCGCCTTGCCAGCCCGCTCAG-3') adaptor and a sample specific barcode.