RNA-seq  Caenorhabditis elegans: whole animal
Age synchronized day 1 adult were harvested  for RNA extraction. Total RNA was  extracted.Sample lysis was performed using a Tissuelyser and a  Trizol-chloroform based method was used in conjunction with the Zymo RNA Clean  & Concentrator kit to obtain RNA.  The Illumina TruSeq stranded mRNA kit was used to obtain stranded mRNA  via Oligo-dT bead capture, and cDNA libraries were prepared from 500ng RNA  per sample. Each sample was multiplexed on 6 lanes of the Illumina HiSeq 4000  sequencer, generating 2x150bp paired end reads, with about 43 to 73 million  reads per sample. The reads were trimmed of adapters contamination and we  removed 20 bp from the 5 and 3 ends using TrimGalore Version0.6.0. Only  reads with a base quality of >Q25 were  maintained.