GSM1833703: SeqCapEpi-enriched Bsseq MCF7 normoxia, exp 2; Homo sapiens; Bisulfite-Seq
Nucleic acids were extracted using the Wizard Genomic DNA Purification (Promega, Leiden, The Netherlands) kit according to instructions, with all buffers supplemented with DFO (200 µM), dissolved in 80 µL PBS-DFO with RNAse A (200 units, NEB, Ipswich, MA, USA), incubated for 10 minutes at 37°C. After proteinase K addition (200 units) and incubation for 30 minutes at 56°C, DNA was purified using the QIAQuick blood and tissue kit (all buffers supplemented with DFO), eluted in 100 µL of a 10 mM Tris, 1mM EDTA solution (pH 8) and stored at -80°C until further processing. Library preparations and enrichment using capture probes were done using the SeqCap Epi Enrichment System (V1.0) according to manufacturers instructions.
GEO Accession
GSM1833703