GSM2443532: OvW petals; Orychophragmus violaceus; RNA-Seq
The petals on half-open flowers were collected in liquid nitrogen from the four samples, respectively, and kept at -80°C. Total RNA was extracted by guanidine isothiocyanate method and then treated with RQ1 DNase (Promega) to remove DNA. Polyadenylated mRNAs were purified and concentrated with oligo(dT)-conjugated magnetic beads (Invitrogen) before used for directional RNA-seq library preparation. Purified mRNAs were iron fragmented at 95°C followed by end repair and 5' adaptor ligation. Reverse transcription was performed with RT primer harboring 3' adaptor sequence and randomized hexamer. The cDNAs were purified and amplified and PCR products corresponding to 200-500 bps were purified, quantified and stored at -80°C until used for sequencing.
GEO Accession
GSM2443532