GBS of coconut: BC1 progeny of MYD female parent 132 09 10 and F1 MYD x WAT male parent 115 02 24
Genotyping-by-Sequencing (GBS with PstI-MseI and library plate: GBS4) of coconut: first backcross progeny of MYD (Malayan Yellow Dwarf mother) female parent and F1 MYD x WAT (West African Tall) male parent (pollen donor). After identifying reads belonging to both parents and the progeny from the sequencing pools using barcode nucleotides and demultiplex.py program in a software package https://github.com/timflutre/quantgen, reads were cleaned (trim the Illumina adaptor sequences) with the arcad_hts_1_cutadapt_in_chain.pl and arcad_hts_2_Filter_Fastq_On_Mean_Quality.pl programs in a pipeline software package https://github.com/SouthGreenPlatform/arcad-hts.