RNA-Seq of the seaworm: body muscle
Total RNA was isolated with Trizol reagent (Invitrogen, Carlsbad, USA) according to manufacturers protocol.  Poly (A) mRNA was purified from the total RNA using oligo (dT) magnetic beads. Equal amounts of the high-quality mRNA samples were obtained from each group for cDNA library preparation using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) and purified using Agencount AMPure XP beads (Beckman Coulter, Krefeld, Germany) according to the manufacturer's recommendations. The concentration of the cDNA library was determined with Agilent Bioanalyzer 2100 system. A transcriptome sequencing of the library preparations was carried out on an Illumina HiSeq 2000 platform by Novogene (Beijing, China) to obtain paired-end reads.