P. aeruginosa PAO1 from female C3H mice (n=8); cecal contents; Replicate 2
P. aeruginosa total RNA was recovered from cecal contents of a mouse colonized with Pseudomonas aeruginosa PAO1. Prokaryotic RNA was enriched and isolated using the MICROBEnrich Kit (Ambion). Ribosomal RNA was then removed from prokaryotic total RNA using the RiboMinus Kit (Life Technologies).