Low-quality isoforms from PacBio sequencing of Brugia malayi L3 RNA
Total RNA from B. malayi adult males and females was obtained from the FR3. RNA quality was assessed by 2100 Bioanalyzer, converted to single stranded cDNA and amplified using the SMARTer PCR cDNA Synthesis Kit, and IsoSeq libraries were constructed with equimolar cDNA fractions (0.5X and 1X) with the SMRTbell Template Prep Kit 1.0. Library quantity and quality were assessed by Qubit HS DNA and 2100 Bioanalyzer. Isoforms were identified from subreads files with IsoSeq2.