Illumina Genome Analyzer IIx sequencing; MYCN regulated miRNAs
MYCN regulated miRNAs
Un-induced SY5Y-MYCN cells and cells treated with 1ug/ml Doxycycline (Sigma) to induce MYCN overexpression, for 1h, 4h 24h were used. For the time-course treatment start times were staggered so all samples were lysed simultaneously. Total mRNA was extracted using TRI Reagent (Sigma-Aldrich) according to manufacturer’s protocol, and DNA was digested with DNA-free Kit (Applied Biosystems). miRNA  libraries (SY5Y-MYCN; un-induced, 1h, 4h and 24h MYCN induction) were prepared using TruSeq small RNA sample preparation kit (Illumina).
Experimental Factor: time
4 hour
Experimental Factor: phenotype
doxycycline-mediated overexpression of MYCN