Ribosome profiling of Mus Musculus: adult female B cells
Cells treated with or without GC7 were lysed and spiked with yeast lysate. mRNA was purified with oligo-dT and randomly fragmented. Together with RPF miRNA, the fragmented mRNA was ligated with the linker (AppCTGTAGGCACCATCAAT/3ddC), reverse transcribed with the RT Oligo (ATCTCGTATGCCGTCTTCTGCTTG) and rRNA-depleted. Semi-quantitative PCR was used for amplification of the library.