WES-Homo Sapiens
The qualified genomic DNA samples were randomly fragmented by Covaris and the size of the library fragments was distributed predominantly between 150 and 200 base pairs (bp). Adapters were then ligated to both ends of the resulting fragments. The adapter-ligated templates were purified by the Agencourt AMPure SPRI beads and fragments with insert size about 176 bp were excised. Extracted DNA was amplified by ligation-mediated PCR (LM-PCR), purified, and hybridized to the SureSelect Biotinylated RNA Library (BAITS) for enrichment. The hybridized fragments were bound to strepavidin beads whereas non-hybridized fragments were washed out after 24 h. CapturedLM-PCR products were subjected to Agilent 2100 Bioanalyzer to estimate the magnitude of enrichment. Each captured library was then loaded on HiSeq2000 platform, and high-throughput sequencing for each captured library was performed.