GSM4698355: CS_dissection; Homo sapiens; OTHER
Total RNA was extracted using RNeasy Micro kit (Qiagen). The CDR3 region of the T-cell receptor (TCR) chain was amplified using the iRepertoire multiplex primer set (HTBI-M; iRepertoire, Huntsville, AL, USA) following the manufacturer's instructions. The Qiagen OneStep RT-PCR kit (Qiagen, Valencia, CA, USA) was used for reverse transcription. Amplification and library preparation were performed with the iR-Processor and iRock 2.0 (iRepertoire, Huntsville, AL, USA).  Paired-end sequencing was performed on the purified libraries using the Illumina MiSeq v2 500-cycle Reagent Kit (Illumina, San Diego, CA, USA).
GEO Accession
GSM4698355