GSM4251667: SEM-R1-End; Homo sapiens; OTHER
Cell pellets were harvested in biological duplicates and flash frozen. Genomic DNA  was harvested using Machery Nagel Blood XL kit. Illumina sequencing library was generated using custom PCR primers to amplify the sgRNA sequences from total isolated genomic DNA.  sgRNA sequences served as barcodes for analysing the changes in guide abundance during the course of the experiments
GEO Accession
GSM4251667