Pooled sample consisting of samples: Cyst 865 S1|Cyst 881 S1|Cyst 818 S1|Cyst 834 S1|Cyst 819 S1|Cyst 827 S1|Cyst 883 S1|Cyst 828 S1|Cyst 836 S1|Cyst 852 S1|Cyst 860 S1|Cyst 829 S1|Cyst 845 S1|Cyst 861 S1|Cyst 869 S1|Cyst 877 S1|Cyst 822 S1|Cyst 894 S1|Cyst 808 S1|Cyst 832 S1|Cyst 848 S1
Primers were used to amplify DNA in 25-uL multiplex polymerase chain reactions (PCRs) as described previously 15.  For each sample, three multiplex PCRs were performed, with each multiplex PCR containing 22 to 50 primer pairs.  Reactions were purified with AMPure XP beads (Beckman Coulter) and eluted in 100 uL of Buffer EB (Qiagen, cat. no. 19086).  0.25% of the purified PCR products were then amplified in a second round of PCR, as described in Springer et al.15.  The PCR products were purified with AMPure and used for sequencing on a MiSeq or HiSeq instrument