allopatric 4
We extracted genomic DNA with the FastDNA (MP Biomedicals, CA, USA) kit.  We collected, dried, and ground the flag leaf of each specimen using the FastPrep tissue grinder and used 50 to 150 mg of dried leaf material for DNA extraction. We sequenced nine individuals from 5ug of purified gDNA on an Illumina HiSeq 2000 using paired-end 100bp reads at the Functional Genomics Laboratory at the University of California, Berkeley.