Pool-seq of Carassius auratus: adult male and female finclips
Pool-seq libraries were generated  using the Truseq nano DNA sample prep kit (Illumina, ref. FC-121-4001)  following the manufacturers instructions. Briefly, each pool was sonicated  using a Bioruptor (Diagenode). The sonicated pools were repaired, size selected  on magnetic beads aiming for a 550 pb insert size and adenylated on their 3  ends. Adenylated DNA was ligated to Illuminas specific adapters and, after  purificaiotn on magnetic beads, was amplified in an 8 cycles PCR. Libraries  were purified using magnetic beads, checked on a Fragment Analyzer (Agilent)  using the HS NGS Fragment kit (DNF-474-33) and quantified by qPCR using  the KAPA Library quantification kit  (Roche, ref. KK4824). Each libraries was sequenced on half a lane of a rapid  v2 flow cell (Illumina) in paired end 2*250nt mode.