PAT-seq rep2 of MB-3 treated Col-0
Seeds were geminated on 1/2MS for 3 days, and were transferred to 1/2MS contained 10 M MB-3. Two weeks old seedlings were collected for RNA extraction. Total RNA was fragmented by 5X 1st strand buffer. Poly(A) tags were enriched by oligo dT. Enriched poly(A) tags were reverse transcribed for library prepration. Size ragne from 300 to 501 bp of final produces were gel purified for sequencing.