Complete genome sequencing of Synsepalum dulcificum Fresh leaf tissues
High Molecular weight genomic DNA was  extracted from the leaf material using a modified CTAB extraction protocol .  The genomic DNA was sheared to 20kb     and fragments shorter than 7 kb were filtered using BluePippin. The  filtered DNA was the nused    to prepare a proprietary SMRTbell library using the PacBio DNA    Template Preparation Kit .