GSM4459995: N642MR2; Homo sapiens; OTHER
Cells were lysed gently and small molecular weight DNA, consisting mostly of excised 26-27 mers was separated from chromosomal high molecular weight DNA by centrifugation Briefly, the excision products were isolated by TFIIH immunoprecipitation following gentle cell lysis and non-chromatin fraction separation, and ligated with adaptors. The ligated excision products were then further purified by immunoprecipitation with anti-(6-4)PP antibody and repaired by (6-4)PP photolyase before the library amplification by PCR. Libraries were sequenced on an Illumina HiSeq 4000 platform. XR-seq
GEO Accession
GSM4459995