DNA from deep-sea seep water
Water was filtered (0.22) and filter stored at -80C until DNA extraction. DNA was extracted using a MoBio Powersoil kit (MO BIO Laboratories Inc., Carlsbad, CA, USA). Preparation for sequencing of the V4 region of the 16S rRNA gene was carried out according to the Earth Microbiome Project protocol (Caporaso et al., 2011; Caporaso et al., 2012) with modifications as previously described (Case et al., 2015). Raw sequences were generated on an Illumina MiSeq platform at Laragen, Inc. (Los Angeles, CA, USA).