Hic of Camellia sinensis: leaf
About 3g young leaves were harvested after two days shading treatment. After cutting the plant leaves into fragments of about 4 mm2, we added 50 mL 2% formaldehyde into a container with sample fragments. The mixture was placed at room temperature for 15 min with intermittent agitation. Following procedures are: add 2.5 ml 2M glycine, and place it at room temperature for 5 min, and place it on ice for at least 15min to terminate the crosslinking; filter the cross-linked plant tissue solution with two layers of gauze, discard the solution and leave solid plant tissue; freeze the tissue sample after cross-linking by liquid nitrogen and stored in -80 for libraries construction. Nuclei extraction, nuclei permeabilization, chromatin digestion and proximity-ligation treatments were performed.