GSM4253657: Old_5541_P2_14; Homo sapiens; RNA-Seq
1mg/ml BSA Lysis buffer (Ambion Thermo Fisher Scientific, Waltham, MA, USA); Single cell cDNA libraries were using the SMARTSeq v2 protocol (Picelli et al., 2014) with the following modifications: Use of 250 pg cDNA with 1/5 reaction of Illumina Nextera XT kit (Illumina, San Diego, CA, USA). All samples were subjected to an indexed paired-end sequencing run of 2x51 cycles on an Illumina HiSeq 2000 system (Illumina, San Diego, CA, USA) (192 samples/lane).
GEO Accession
GSM4253657