NextSeq 500 paired end sequencing; Single-nucleotide resolution mapping of DNA abasic sites by snAP-seq
Single-nucleotide resolution mapping of DNA abasic sites by snAP-seq
Leishmania DNA was purchased from ATCC (product 30012D, lot 62762024). Purified and sonicated DNA was treated with UNG (10 U, NEB) for 18 hr at 37 °C. Input libraries were prepared for each enriched (snAP-seq) library. DNA was end-prepped using an NEBNext Ultra II kit, followed by adapter ligation using Truseq LT indexed adapters (Illumina). PCR amplification conditions were kept consistent with corresponding enriched (snAP-seq) libraries.
Experimental Factor: RNA interference
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Experimental Factor: stimulus
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