RNA-Seq of Bos taurus: ruminal protozoa
The protozoal pellet was thawed and RNA extracted using the FastRNA Pro-Soil Direct kit (MP Biomedicals) according to the manufacturers guidelines. RNA concentration was quantified using the Epoch Micro-Volume Spectrophotometer (Biotek). The Poly(A) Purist MAG kit (Life Technologies) was used to select for Polyadenylated mRNA, following the manufacturer's guidelines. To remove any contaminating DNA prior to cDNA synthesis, a TURBODNA-free kit (Thermo-Fisher Scientific) was used, following manufacturers guidelines.