GSM1181834: H111 wt micro-oxic; Burkholderia cenocepacia H111; RNA-Seq
Cells were flash frozen on dry ice, and RNA was harvested using hot phenol protocol (Pessi et al. 2007) and DNA was removed. First-strand cDNA synthesis was performed using an oligo(dT)-adapter primer and the M-MLV reverse transcriptase. The resulting cDNA was PCR-amplified to about 20-30 ng/μl using a high fidelity DNA polymerase. The cDNA was purified using the Agencourt AMPure XP kit (Beckman Coulter Genomics) and was analyzed by capillary electrophoresis. The primers used for PCR amplification were designed for TruSeq sequencing according to the instructions of Illumina. The following adapter sequences flank the cDNA inserts: TrueSeq_Sense_primer 5' ́-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3'; TrueSeq_Antisense_NNNNNN_primer Barcode 5'-CAAGCAGAAGACGGCATACGAGAT-NNNNNN-GTGACTGGAGTTCAGACGTGTGCTCTTCCGATC(dT25)-3'.
GEO Accession
GSM1181834