GSM3659308: PaS cells – Ebf1fl/fl;Prx1+/cre – ko Replicate 3; Mus musculus; RNA-Seq
RNA-seq: Total RNA was isolated from the FACS sorted CAR and PaS cells using RNeasy Micro Kit (Qiagen) according to the manufacturer's instructions. The total mRNA was enriched by using Oligo dT magnetic beads. ATAC-seq: minimum of 10.000 cells were resuspended in 50 µL of cold lysis buffer (10 mM Tris·Cl at pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% [v/v] Igepal CA-630) and incubated for 15 min on ice. The supernatant was discarded after centrifugation, and the nuclei were used for transposition reaction using NEB Nextera DNA sample preparation kit. The RNA-seq libraries were prepared by using SMART-Seq v4 ultra-low input RNA library preparation protocol according to the manufacturer's instructions. The ATAC-seq libraries were prepared using the NEB Nextera DNA sample preparation kit according to the manufacturer's instructions.
GEO Accession
GSM3659308