Differentiation of mesenchymal stem cells into adipocytes.
Differentiation of mesenchymal stem cells into adipocytes.
The total and polysomal RNA fractions were extracted by a standard Trizol (Invitrogen) RNA isolation protocol. We subjected total and polysome-associated RNA samples to amplification with the Amino Allyl Message Amp II aRNA Amplification Kit (Ambion), to provide a template for SOLiD libraries. The cDNA libraries were prepared with the SOLiD Whole Transcriptome Analysis Kit and the purified products were evaluated with an Agilent Bioanalyzer (Agilent). Library molecules were subjected to clonal amplification according to the SOLiD Full-Scale Template Bead preparation protocol and sequenced with the SOLiD4 System (Applied Biosystems).
Experimental Factor: cell type
undifferentiated mesenchymal stem cell