GSM1468718: 4h-post-CIP_IP; Mus musculus; ChIP-Seq
Chromatin samples were prepared using pancreatic tissue obtained from 2-4 months old mice injected with cerulein. Chromatin Immunoprecipitation was carried out using Anti-ATF3 (sc-188; Santa Cruz). After reverse cross-linking and treatment with Proteinase K, the samples were treated with Phenol Chloroform twice. The DNA was Ethanol precipitated, washed with 70% Ethanol. Samples were eluted and pooled in 40ul of H2O. This eluted DNA was sent for sequencing. Library was constructed by the Beijing Genomics Institute (Hong Kong), following their established protocol (http://www.genomics.cn/en/).
GEO Accession
GSM1468718