GSM4210336: bone marrow DC progenitors, Cl13 rep1 (ATAC-seq); Mus musculus; ATAC-seq
Lin-c-Kitint/loFlt3+ cells (Lin includes B cells (CD19, B220), T cells (Thy1.2, CD3, CD4, and CD8), NK cells (NK1.1), red  blood cells (Ter119), granulocytes (Gr-1), and monocytes (CD11b)) were FACS-purified from bone marrow. Purified cells were resuspended in lysis buffer and spun down 600g for 30 min at 4°C. Nuclei pellet was resuspended into transposition reaction mixture containing Tn5 transposase from Nextera DNA Sample Prep Kit (Illumina) and incubated for 30 min with gentle shaking at 37°C. ATAC-seq library was constructed by Nextera DNA Sample Prep Kit (Illumina). Following purification, we first amplified the full libraries for 5 cycles and the additional number of cycles needed for the remaining 45 μL reaction was determined as published in the original protocol (Buenrostro et al., 2013) . The libraries were purified using a Qiagen PCR cleanup kit in 20 μL. Libraries were amplified for a total of 10–12 cycles. The library was pooled and size-selected to range between 100bp and 600bp by using PippinHT (Sage Sciences). Samples were subsequently analyzed on a HiSeq 4000 instrument using a single read 100bp protocol.
GEO Accession
GSM4210336