Emergence of Clostridium novyi infection and mortality in a free ranging wild Asian elephant population, Southern India
DNA was extracted from blood of Tongue tissue of Elephas maximus indicus using DNA Isolation kit (MO BIO Laboratories, Carlsbad, CA). We designed and synthesized primers needed for amplification of 16S V3-V4 region and build a library and sequenced on Illlumina HiSeq 2500 platform. The samples were then used as template for PCR amplification. Libraries from the sample was tracked using a sequence barcode that was incorporated in the libraries generated. Bioinformatics QIIME pipeline was used to identify the presence of Clostridium novyi species.