Illumina HiSeq 2500 paired end sequencing; ChIP-seq for Meis on mouse branchial arches at E11.5
ChIP-seq for Meis on mouse branchial arches at E11.5
The preparation of the material was as described in Kutejova et al., 2008. Second branchial arches, and posterior branchial arches connected to OFT were dissected (the whole tissue), crosslinked and frozen in order to pool enough for ChIP. Kutejova E, Engist B, Self M, Oliver G, Kirilenko P, Bobola N. Six2 functions redundantly immediately downstream of Hoxa2. Development. 2008. 135(8):1463-70. PMID: 18321982. MinElute PCR purification kit (Qiagen) ChIP-seq was performed as described in Donaldson et al., 2012, using 20ug of Meis antibody (Santa Cruz Biotechnology sc-10599X). Donaldson I, Amin S, Hensman J, Kutejova E, Rattray M, Lawrence N, Hayes A, Ward C and Bobola N. Genome-wide occupancy links Hoxa2 to Wnt-beta-catenin signaling in mouse embryonic development. Nucleic Acids Res. 2012. 40(9):3990-4001. 70 arches were used for each sample. Library preparation was performed using the TruSeq ChIP Sample Preparation kit (Illumina).
Experimental Factor: second branchial arch
organism part
Experimental Factor: anti-Meis
immunoprecipitate