GSM1468816: DICER1-ifKO smallRNA rep3; Mus musculus; ncRNA-Seq
Total hippocampal RNA was extracted with TRI reagent (Sigma). Total RNA extracted was treated with DNaseI (Qiagen) and precipitated using potassium acetate. All samples were tested by qRT-PCR for recombination. To prepare each sample equal amounts of purified total RNA from 3 mice were pooled together. 3 samples (biological replicates) per genotype were sequenced in HiSeq 2500 apparatus (Illumina). Service provided by Fasteris S.A. RNA libraries were prepared for sequencing using standard Illumina protocols (Small RNA TruSeq Protocol). Service provided by Fasteris S.A.
GEO Accession
GSM1468816