D. koepferae ovaries RNA-seq
Total RNA was purified from 20 pairs of D. koepferae ovaries with the Nucleospin RNA purification kit (Macherey-Nagel). RNA quality and concentration was evaluated using Experion Automated Electrophoresis System (Bio-rad). An Illumina library of 250-300bp fragments was prepared using 2μg of purified RNA. Sequencing was performed using the Illumina mRNA-seq paired-end protocol on a HiSeq2000 platform, at the INRA–UMR AGAP (Montpellier, France).