BGISEQ-500 sequencing; RNA-seq of human melanoma cell line M381 treated with CAY or co-treated with CAY and oleic acid in comparison with untreated control
RNA-seq of human melanoma cell line M381 treated with CAY or co-treated with CAY and oleic acid in comparison with untreated control
Cell under their treatment condition were trypsinized off the culture plate and cell pellets were harvested for later RNA extraction.  Cells were cultured in RPMI 1640 (Gibco, 11875119), supplemented with 10% fetal bovine serum (Omega, FB-12), and 0.2% MycoZap Plus-CL antibiotics (Lonza, VZA-2011). Cultures were incubated in a water-saturated incubator at 37°C with 5% CO2. Cells were maintained and tested for mycoplasma using kit (Lonza, LT07-118). Compound were dissolved in DMSO (ATCC, 4-X) at designated concentrations before adding to cell culture media. Total RNA was extracted from frozen cells pellets (~ 1 million cells) using the RNeasy MicroKit (Qiagen, 74004) according to the manufacturer's protocol. The library preparation was followed by BGI's standard procedure.
Experimental Factor: compound
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