GSM4210271: Rif1nullCyo_OvarianFollicleCell_MS_Replicate1; Drosophila melanogaster; OTHER
Adult female flies were fattened for 2 days on wet yeast and ~200 pairs of ovaries were dissected out by hand dissection. Tissue was disrupted by douncing and filtered over a 30 mM filter to collect follicle cell nuclei. DAPI-stained nuclei were sorted by 5-Laser FACS Aria III and G1 and S-phase populations were collected. Genomic DNA was fragmented by sonication and extracted by using a standard Phenol-Chloroform extraction protocol and precipitated with sodium acetate, glycogen, and isopropanol. Libraries were prepared according to the protocol accompanying NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (E7645).
GEO Accession
GSM4210271