Exploring candidate genes for pericarp russet pigmentation of sand pear (Pyrus pyrifolia)  via Bulked Segregant RNA-seq Analysis
Pericarp of either russet- or green-pericarp offspring from ‘Qingxiang’ * ‘Cuiguan’ F1 group were collected and bulked at 80-d after flowering. The bulke tissues were immediately frozen in liquid nitrogen until RNA extraction. The cDNA libraries were constructed for each pericarp pool using the TruSeqTM RNA Sample Preparation Kit (Illumina,  Inc.) according to the manufacturer’s instructions.