WGS of Pectobacterium brasiliense: strain IPO:4062 NAK:237
Barcoded SMRT bell libraries were  prepared according to manufacturers protocol (PacBio). In short, DNA of  individual isolates was subjected to damage repair (NEBNext FFPE) repair and  sheared using g-tubes (Covaris) to approximately 10 Kb peak fragment size.  DNA size was analysed on a Fragment Analyzer (Agilent) and quantified using  Qubit (Life Technologies). SMRT bell DNA polymerase binding complexes (v2.0)  were made of pool and finally loaded by diffusion (Sequencing chemistry 2.1,  5 to 10 pM loading). Sequencing was done for 10 hours movie time per cell  after 120 minutes immobilization time. on a Sequel system (PacBio, USA).