GSM1466830: Human ESCs MS46 day1; Homo sapiens; RNA-Seq
RNeasy kit (Qiagen) for RNA-seq; 1% formaldheyde crosslinked chromatin for ChIP-seq Poly-A+ selection (magnetic oligodT-containing beads)  was used for RNA-seq library preparation. cDNA preparation and library construction was performed as described by the manufacturer (Illumina, CA, USA) . RNA libraries were sequenced using Genome Analyzer IIx System 50bp single-read method. Llibraries for ChIP-sequencing were generated as described in (Ntziachristos et al., 2012), including end-repair, A-tailing, adapter (Illumina Truseq system) ligation and PCR amplification of the libraries. We performed cluster amplification and 50-nucleotide single-read sequencing using the Illumina HiSeq 2000, following manufacturer’s protocols. Library construction was performed as described by the manufacturer (Illumina, CA, USA)
GEO Accession
GSM1466830