GSM4459839: MT-siHRP2-HRP2-input; Mus musculus; ChIP-Seq
For TY1,HRP2 and DPF3a ChIP, cells were double cross-linked with DMA and 1% formaldehyde. For H3K36me2 ChIP, cells were cross-linked with 1% formaldehyde. The crosslink was stopped by adding  2.5 M glycine. Cells were washed with PBS and harvested using ChIP lysis buffer. Cells were then sonicated to obtain fragments (100-500 bp) with Bioruptor Sonicator. Immunoprecipitation was performed withTY1, HRP2 (15134-1-AP), DPF3a and H3K36me2 (ab9049). After elution and reversal cross-linking, DNA was purified and sequenced on HiSeq X ten. Construction of ChIP-seq library was performed using VAHTSTM Universal DNA Library Prep Kit for Illumina® V3  (Vazyme).
GEO Accession
GSM4459839