GSM4698631: CD8_m4; Mus musculus; Bisulfite-Seq
Genomic DNA was isolated by phenol-chloroform extraction followed by ethanol precipitation. Two aliquots of DNA were separately digested with either TaqI or MspI restriction enzymes over night and subsequently pooled for all downstream processing.  Digested DNA was end-repaired, A-tailed, and adapters containing methylated cytosines ligated on using the KAPA HyperPrep kit according to the manufacturers instructions.  Adapter-ligated DNA was bisulfite converted over using the EpiTech Bisulfite kit Qiagen, Inc.  Bisulfite treated libraries were PCR amplified using indexed primers and the HiFi HotStart Uracil+ polymerase KAPA Biosystems.
GEO Accession
GSM4698631