Pooled sample consisting of samples: Cyst 825 S1|Cyst 826 S1|Cyst 842 S1|Cyst 850 S1|Cyst 866 S1|Cyst 874 S1|Cyst 843 S1|Cyst 812 S1|Cyst 844 S1|Cyst 892 S1|Cyst 821 S1|Cyst 837 S1|Cyst 885 S1|Cyst 814 S1|Cyst 854 S1|Cyst 862 S1|Cyst 878 S1|Cyst 863 S1|Cyst 871 S1|Cyst 824 S1|Cyst 864 S1
Primers were used to amplify DNA in 25-uL multiplex polymerase chain reactions (PCRs) as described previously 15.  For each sample, three multiplex PCRs were performed, with each multiplex PCR containing 22 to 50 primer pairs.  Reactions were purified with AMPure XP beads (Beckman Coulter) and eluted in 100 uL of Buffer EB (Qiagen, cat. no. 19086).  0.25% of the purified PCR products were then amplified in a second round of PCR, as described in Springer et al.15.  The PCR products were purified with AMPure and used for sequencing on a MiSeq or HiSeq instrument