mRNA-Athabasca-biofilms
Total rRNA-subtracted RNA was reverse-transcribed using the SuperScript III kit (Invitrogen). Illumina libraries were prepared following the protocol of Meyer and Kircher (33), with indices 7 to 42 pooled together and sent for three lanes of Illumina HiSeq 2000 paired-end 2x101 bp sequencing at The Center for Applied Genomics of the Hospital for Sick Children, Toronto, Canada. Resulting data were split into 216 files (36 samples x 2 reads x 3 lanes).