Sediment-7
DNA extraction was made using the Quick DNA Fecal/Soil Microbe Kits by Zymo Research according to manufacture instructions. DNA extractions were sent to the Iowa State University (DNA facility) for library preparation and sequencing on the Illumina Miseq Platform. The desired region for amplification was the V4 hypervariable region of the 16S rRNA gene using the primer pairs 515F and 806R