Illumina HiSeq 2000 paired end sequencing; Regulation of constitutive and alternative messenger RNA splicing across the human transcriptome by PRPF8 is determined by 5' splice site strength
Regulation of constitutive and alternative messenger RNA splicing across the human transcriptome by PRPF8 is determined by 5' splice site strength
Human Cal51 breast carcinoma cells were treated with either control siRNA or PRPF8 siRNA and harvested 54 hours post-transfection for total RNA. 4 experimental replicates for PRPF8 siRNA and 3 experimental replicates for Control siRNA are provided. Total RNA was extracted using Qiagen RNeasy kit according to manufacturers protocol. Illumina's TruSeq RNA-seq kit was used to prepare libraries for sequencing according to manufacturers protocol.
Experimental Factor: sirna treatment
PRPF8
LIBRARY_STRAND
not applicable