RNA-Homo Sapiens
One hundred nanogram of total RNA isolated from a skin biopsy was used to prepare libraries using TruSeq Stranded Total RNA kit (Illumina, CA, USA) following manufacturer's protocol. The final libraries at the concentration of 4 nM were sequenced on NextSeq500 using 75bp paired-end chemistry. Raw FASTQ sequencing reads were mapped against the reference genome of Homo sapiens Ensembl Version GRCh38 utilizing further information from the gene transfer format(.gtf) annotation from GENCODE version GRCh38.p12 using RNA-Seq by Expectation Maximization (RSEM). Total read counts and normalized transcripts per million (TPM) were obtained using RSEM's calculate-expression function.