Pooled sample consisting of samples: Cyst 714 S1|Cyst 718 S1|Cyst 723 S1|Cyst 725 S1|Cyst 728 S1|Cyst 729 S1|Cyst 731 S1|Cyst 748 S1|Cyst 755 S1|Cyst 756 S1|Cyst 762 S1|Cyst 768 S1|Cyst 772 S1|Cyst 777 S1|Cyst 779 S1|Cyst 789 S1
Primers were used to amplify DNA in 25-uL multiplex polymerase chain reactions (PCRs) as described previously 15.  For each sample, three multiplex PCRs were performed, with each multiplex PCR containing 22 to 50 primer pairs.  Reactions were purified with AMPure XP beads (Beckman Coulter) and eluted in 100 uL of Buffer EB (Qiagen, cat. no. 19086).  0.25% of the purified PCR products were then amplified in a second round of PCR, as described in Springer et al.15.  The PCR products were purified with AMPure and used for sequencing on a MiSeq or HiSeq instrument