GSM4648196: HC3; Homo sapiens; RNA-Seq
BM mononuclear cells (BMMNC) were isolated by HISTOPAQUE® (Sigma, Cat# 10771) density gradient centrifugation The single cells were obtained by a mouth pipette from sorted, bulk BFU-E and CFU-E cells.Sequencing libraries were constructed following a modified Smart-seq2 protocol as previously reported(Li et al., 2017); After RT and 18 cycles of PCR amplification, 3ml aliquots from each single cell cDNA-amplicon was pooled and purified using DNA Clean & Concentrator™ kit (ZYMO research, Cat# D4014) and AMPure XP Beads (Beckman, Cat# A63882). Library preparation was performed using 2X KAPA HiFi HotStart ReadyMix kit after biotin-indexing PCR.
GEO Accession
GSM4648196