Pooled sample consisting of samples: CystA 1041 S1|CystA 1002 S1|CystA 1034 S1|CystA 1090 S1|CystA 1003 S1|CystA 1035 S1|CystA 1043 S1|CystA 1075 S1|CystA 1083 S1|CystA 1076 S1|CystA 1084 S1|CystA 1013 S1|CystA 1021 S1|CystA 1029 S1|CystA 1070 S1|CystA 1023 S1|CystA 1031 S1|CystA 1047 S1|CystA 1063 S1|CystA 1079 S1|CystA 1087 S1
Primers were used to amplify DNA in 25-uL multiplex polymerase chain reactions (PCRs) as described previously 15.  For each sample, three multiplex PCRs were performed, with each multiplex PCR containing 22 to 50 primer pairs.  Reactions were purified with AMPure XP beads (Beckman Coulter) and eluted in 100 uL of Buffer EB (Qiagen, cat. no. 19086).  0.25% of the purified PCR products were then amplified in a second round of PCR, as described in Springer et al.15.  The PCR products were purified with AMPure and used for sequencing on a MiSeq or HiSeq instrument