GSM4384985: WGBS H_C; Homo sapiens; Bisulfite-Seq
PBMCs from each donor were obtained by using the Ficoll density gradient separation method (Sigma, USA). Then PBMCs was stored at –80 °C for further treatment. Moreover, genomic DNA was extracted from PBMCs using a DNA isolation kit for cells. Total DNA was extracted using QIAamp Fast DNA Tissue Kit (Qiagen, Dusseldorf, Germany) following the manufacturer's procedure.The Accel-NGS Methyl-Seq DNA Library Kit (Swift, MI, USA) was utilized for attaching adapters to single-stranded DNA fragments.. Finally, we performed the pair-end 2×150bp sequencing on an illumina Hiseq 4000 platform housed in the LC Sciences.
GEO Accession
GSM4384985