Single Cell RNA-Seq of a whole organism of zebrafish larva
Zebrafish embryo at one-cell stage were micro-injected with gRNA pool and Cas9 mRNA mixture, and growth at standard condiction to 7dpf. Then a single larva were lysis into single cell suspension and the RNA profile were obtained by 10x Genomics Chromium Single Cell 3' Reagent Kits (v2 Chemistry).