RNA-Seq of human brain sample from an individual human
RNAs was obtained directly from Life Technologies, First Choice, and used as input for TruSeq Stranded mRNA-seq kit (Illumina) with parameters set for ~150 bp insert size libraries. Final purified libraries were assayed both by fluorometer (Qubit, ThermoFisher) for dsDNA concentration and Bioanalyzer (DNA12000 chip, Agilent) for size.