Genome-wide CRISPRn screen of homo sapiens: K562 cell growth
Genomic DNA was extracted from K562 cells containing the CRISPRn sgRNA genome-wide library using the Qiagen Blood Maxi Kit. Nested PCR was used to amplify the sgRNA cassette and attach Illumina adapters. Libraries were purified, quantified, multiplexed and sequenced on an Illumina NextSeq 500.