GSM4211262: EZH2 dCLIP rep#2; Mus musculus; OTHER
UV cross-linking UV-cross-linked cells, expressing BirA biotinylase and Avi-GFP-tagged EZH2, SUZ12  or mock vector, were isolated and lysed. Lysates were DNase-treated and RNAse-treated. Cleared lysates were incubated 2hrs with Streptavidin beads. After stringent washing and 2nd DNase treatment, CLIP-tags on beads were phosphorylated, cross-linked RNA was eluted by proteinase K, followed by Trizol extraction and ethanol precipitation. Multiplex Compatible NEBNext Small RNA Library Prep Set for Illumina (NEB). Illumina barcode #8, ACTTGA. Paired-end 50bp reads dCLIP-seq
GEO Accession
GSM4211262