GSM3780566: apa_array: human_custom_library; Homo sapiens; RNA-Seq
RNA was extracted from transiently transfected cells 36-48 hours later using the NEB Monarch RNA Purification kit. The purified RNA was then polyA purified using the NEB PolyA Spin kit (magnetic-bead-based protocol). Library specific priming sites were used to append Illumina adapter sequences and to amplify the library. Amplification was conducted in a light-cycler and samples removed early to minimize PCR bias.