GSM3204344: K562-Y518R; Homo sapiens; RNA-Seq
total RNAs were isolated using Direct-zol RNA Extraction Kit (Zymo Research). Library preparation and sequencing for RNA samples were performed at Genewiz (South Plainfield, NJ). Briefly, the quality of the total RNA was assessed using Nanodrop 2000, Qubit RNA Assay, and TapeStation). One round of polyA+ selection was performed for RNA samples, followed by conversion to cDNAs. The mRNA library was generated using NEBNext Ultra RNA Library Prep KitA as per manufacturer's instructions. The indexed DNA libraries were quantitated using qPCR in a Roche LightCycler 480 with the Kapa Biosystems kit for library quantitation (Kapa Biosystems, Woburn, MA) prior to cluster generation.
GEO Accession
GSM3204344