16S rRNA seq of Salmo salar microbiome: Stomach sample
Stomach content and mucus lining was homogenized and DNA extracted with QIAamp DNA Stool Mini Kit from Qiagen. Primers U786F and A1067R covering the V5/V6 variable region of the 16S rRNA were used. Amplicon fusion primers were designed for 454 sequencing (Roche). Following PCR amplification and verification on agarose gels, the PCR products were purified with the Agencourt AMPure XP PCR purification system to eliminate primer dimers. DNA concentrations were measured and PCR products pooled for sequencing.