Pooled sample consisting of samples: Cyst 681 S1|Cyst 650 S1|Cyst 619 S1|Cyst 651 S1|Cyst 659 S1|Cyst 604 S1|Cyst 620 S1|Cyst 652 S1|Cyst 676 S1|Cyst 629 S1|Cyst 637 S1|Cyst 645 S1|Cyst 606 S1|Cyst 614 S1|Cyst 615 S1|Cyst 623 S1|Cyst 663 S1|Cyst 624 S1|Cyst 632 S1
Primers were used to amplify DNA in 25-uL multiplex polymerase chain reactions (PCRs) as described previously 15.  For each sample, three multiplex PCRs were performed, with each multiplex PCR containing 22 to 50 primer pairs.  Reactions were purified with AMPure XP beads (Beckman Coulter) and eluted in 100 uL of Buffer EB (Qiagen, cat. no. 19086).  0.25% of the purified PCR products were then amplified in a second round of PCR, as described in Springer et al.15.  The PCR products were purified with AMPure and used for sequencing on a MiSeq or HiSeq instrument