GSM2188251: Kidney, normal, pool#2 (n=3 donors)_total RNA; Homo sapiens; RNA-Seq
RNA was extracted from 1 ug of each tissue using TriReagent followed by RNA cleanup using QIAGEN RNeasy Mini Kit with DNase I. Equal amount of RNA from 3 tissue samples (or 5 tissue samples for normal intestine/colon) were pooled and External RNA Controls Consortium (ERCC) standards RNA Spike-In Mix 1 (ThermoFisher Scientific) were spiked into pooled RNA samples. 1 ug total RNA from each pool was used to prepare sequencing librairies with Illumina TruSeq RNA Sample Prep Kit RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM2188251