GSM4210861: EA.hy.RNA.ClincRNA.rep2; Homo sapiens; RNA-Seq
Total RNA was isolated from the cells using RNeasy Mini kit (74104 ;QIAGEN) and DNase treated with RNase-Free DNase Set (79254 ;QIAGEN). Prior to RNA library preparation, the quality of the input RNA was assessed by Fragment Analyzer (Agilent)  using the RNA Kit (DNF-471; Agilent) and a threshold of RQN>9. RNA library preparation was carried out using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7765; New Englands BioLabs) according to manufacturer instructions. Briefly, 0.5 ug of DNAse-treated RNA was subjected to ribosomal RNA (rRNA) depletion, followed by RNA fragmentation, cDNA synthesis and 11 cycles of PCR amplification. The resultant library was quantified using Qubit dsDNA HS Assay Kit (Q32851; ThermoFisher Scientific) and its quality was checked with Bioanalyzer (Agilent). Individual libraries were pooled in equimolar amount (4 nM for each) and sequenced with the Nextseq 550 (Illumina) using single end 75 cycles for 10 million reads per sample. Total RNA was isolated from the cells using RNeasy Mini kit (74104 ;QIAGEN) and DNase treated with RNase-Free DNase Set (79254 ;QIAGEN). The quality of the input RNA was assessed by Fragment Analyzer (Agilent)  using the RNA Kit (DNF-471; Agilent) and a threshold of RQN>9. RNA library preparation was carried out using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7765; New Englands BioLabs) according to manufacturer instructions, using  0.5 ug of DNAse-treated RNA subjected to ribosomal RNA (rRNA) depletion, followed by RNA fragmentation, cDNA synthesis and 11 cycles of PCR amplification. The resultant library was quantified using Qubit dsDNA HS Assay Kit (Q32851; ThermoFisher Scientific) and its quality was checked with Bioanalyzer (Agilent). Individual libraries were pooled in equimolar amount (4 nM for each) and sequenced with the Nextseq 550 (Illumina) using single end 75 cycles for 10 million reads per sample.
GEO Accession
GSM4210861