GSM3780741: D8_CD13+_CD201+_Rep1; Homo sapiens; RNA-Seq
RNA was extracted by the conventional methods using Trizol. Extracted RNA was treated with DNase I to get rid of the contaminant DNA, and purified with the RNeasy mini kit from Qiagen thereafter. RNA-seq libraries were prepared using the TruSeq® Stranded mRNA Library Prep kit following manual guide of the kit. Briefly, mRNA was enriched by the beads conjugated with Poly-T oligo, fragmentized, and reverse transcribed to cDNA. After ligating adaptors, cDNA was further amplified by 15 cycles of PCR. The library was sequenced on HiSeq 4000 sequencer.
GEO Accession
GSM3780741