RNA-seq of Plasmodium falciparum : Schizont
Parasite total RNA was extracted by using a TRIzol kit (Life Technologies) according to the manufacturers instructions. Four micrograms of total RNA was used for mRNA-seq library construction by using a KAPA Stranded mRNA-Seq Kit (KAPA Biosystems, KK8240) according to the manufacturers instructions. Deep sequencing was performed on an HS X10-PE151 (Illumina) at GENEWIZ.