RNA-Seq of Ceriodaphnia quadrangula
Total RNA was extracted using TRIzol. mRNA was purified with magnetic oligo(dt) coated Dynabeads. Fragmented cDNA library with 200 bp fragment sizes was constructed using SuperScript III reverse transcriptase and the PrepX library preparation kit on an Apollo 324. The PrepX kit was used for indexing with indices: AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTG, GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT (indices are reverse-complimented to be used directly in Trimgalore). The library was PCR amplified with the KAPA Library Amplification kit and assessed and quantified with the HSDNA Bioanalyzer and qPCR (KAPA Library Quantification). The speciemen has been registered with the Museum of Comparative Zoology, and can be accessed at http://mczbase.mcz.harvard.edu/guid/MCZ:IZ:50046