Symbion americanus transcriptome
This is an RNA-seq library that was prepared as part of a study on the phylogenetic relationships of Spiralia. Poly-A RNA from a single starved animal in the epizoic phase was converted to cDNA and amplified using a phi29-mRNA rolling-circle-amplification procedure (a la 10.1073/pnas.1217322109). This amplified cDNA was Covaris-fragmented to 800 bp and converted to a sequencable library using forked-adapter TA ligation and subsequent indexing PCR. The library was sequenced in multiplex on an Illumina MiSeq 2x250 bp, but run quality was poor.