Whole Genome Sequencing of Pathan individual
Two paired-end libraries were prepared from 1.1 µg of gDNA using Illumina TruSeq DNA Preparation Kit, following Illumina’s standard protocol (Pair End Library Preparation Kit, Illumina, SanDiego, CA, USA). Shearing of gDNA was done using Covaris S series (Covaris, MS, USA). Following end repair, A-tailing and adaptor ligation, DNA in the 500-600 bp range was purified from a 2% agarose gel.  DNA was then PCR enriched for a total of ten cycles.  Proper DNA size was then confirmed with the Agilent Bioanalyzer, followed by qPCR quantification with Roche Light Cycler 480 II and Kapa Biosystems reagents.