Resequencing genes for urinary calcium excretion
"In order the query the association of rare allelic variation with the degree of urinary calcium excretion, we re-sequenced the exons of 40 candidate genes in 960 well-characterized individuals with either low (n=480) or high (n=480) urinary calcium excretion. We pooled DNA samples of individuals from either group in sets of n=15 or n=20 (total of 54 sample pools) and enriched our target sequence with RainDance droplet-based PCR technology. N=730 samples were enriched successfully according to our quality matrix (n=42 pools). These pools were barcoded and sequenced on the HiSeq 2000 Illumina platform. 615 samples (n=34 pools) reached deep sequence coverage >20x and were analyzed to test our hypothesis. The raw data, which passed our quality matrix and were analyzed, are provided here. Pools 4,5,7-11, 31-37 and 40 are sequence data of individuals with high urinary calcium excretion (total of n=260), whereas pools 15-26, 43, 47, 48 and 50-54 belong to individuals with low urinary calcium excretion (total of n=355)."