GSM4384984: p6_Far_S9; Homo sapiens; RNA-Seq
sample collection protocol: Samples were collected from the surgery room immediately after isolation into sterile saline solution and processed in a sterile cabinet. Each sample was divided equally into two pieces as follows: (1) scRNAseq, samples stored in cold DMEM X1, supplemented with glutamine, (2) frozed section, sample were embedded into OCT block and kept on dry ice to be sectioned using LCM and mcSCRBseq for bulk sequencing cell extract protocol: for scRNAseq, all samples were prepared according to Liver Dissociation Kit (GentelMAX, 130-105-807). Liberase Blendzyme3 recombinant collagenase (Roche Diagnostics) were added to the enzymatic cocktail in the first step in sample preperation protocol for scRNAseq, 3' end mRNA libraries were prepared for sequencing using MARS-seq (Jaitin et al, Science 2014). For bulk analysis, mcSCRBseq protocol was used (Bagnoli et al, Nat Commun. 2018)
GEO Accession
GSM4384984