Pooled sample consisting of samples: CystA 1018 S1|CystA 1058 S1|CystA 1011 S1|CystA 1091 S1|CystA 1020 S1|CystA 1036 S1|CystA 1044 S1|CystA 1068 S1|CystA 1053 S1|CystA 1061 S1|CystA 1006 S1|CystA 1022 S1|CystA 1030 S1|CystA 1046 S1|CystA 1078 S1|CystA 1039 S1|CystA 1032 S1|CystA 1040 S1|CystA 1072 S1|CystA 1079 S1|CystA 1087 S1
Primers were used to amplify DNA in 25-uL multiplex polymerase chain reactions (PCRs) as described previously 15.  For each sample, three multiplex PCRs were performed, with each multiplex PCR containing 22 to 50 primer pairs.  Reactions were purified with AMPure XP beads (Beckman Coulter) and eluted in 100 uL of Buffer EB (Qiagen, cat. no. 19086).  0.25% of the purified PCR products were then amplified in a second round of PCR, as described in Springer et al.15.  The PCR products were purified with AMPure and used for sequencing on a MiSeq or HiSeq instrument