Hypericum perforatum filaments
The messenger RNA (mRNA) from total RNAwas enriched by the use of oligo(dT) magnetic beads for transcriptome analysis. Then, themRNAwas fragmented into short pieces (180–500 bp) with the fragment buffer treatment. The first-strand cDNA was synthesized by random hexamer primer with the mRNA fragments as templates. Buffer, dNTPs,RNase H, and DNA polymerase I was used to synthesize the second strand. The double-stranded cDNAs, purified with QiaQuick PCR extraction kit, were used for the end repair and base A addition. Short fragments were purified by agarose gel electrophoresis after connecting short fragments to sequencing adapters, and enriched by PCR to create the final cDNA library.