GSM3891788: PDX Abemaciclib 1 (H3K27ac FiT-Seq); Homo sapiens; ChIP-Seq
For H3K27acFiT-seq, FFPE sections containing >80% tumor cell enrichment were used. Chromatin was prepared from 10 sections, each 10 μm thick. Sections were washed 3 times with xylenes to remove paraffin, rehydrated in an ethanol/water series, and prepared as previously described5, with modified buffer and sonication conditions (Covaris E220 instrument, 20 min, 5% duty cycle, 105 Peak Incident Power, 200 Cycles per burst, 1mL AFA Fiber milliTUBEs). FiT-seq libraries were constructed using Accel-NGS 2S DNA library kit from Swift Biosciences. ATAC-seq libraries were amplified as described previously (Buenrostro et al., 2015).