Deep sequencing OTC minigene
OTC minigene locus was amplified from gDNA with sequence-specific primers AA_74R 5-TGGAGCTGAGGTGAGTAATCTGT-3 and AA_75F 5-ACCATGCTGTTTAATCTGAGGA-3 located outside of the homology region, to prevent amplification of the donor vector. Purified PCR products were used as template in a second nested reaction, using primers AA_82F 5-TTGCATTGATGTCTGACTACTGG-3 and AA_83R 5-TCCCCAAAGGCATGAATGACCAA-3