SPF_FS5
RNA  samples with RIN values greater than 8 were processed for downstream library  preparation and sequencing. Briefly, 500 ng total RNA per sample extracted  from zooids of Fredericella sultana, were used for library preparation with  the TruSeq RNA Library Prep Kit v2 (Illumina) according to the manufacturers  protocol. Library quality control was done with the D1000 ScreenTape assay  (Agilent, Santa Clara, CA, USA) on the 4200 TapeStation. Sequencing of  libraries (paired-end 150-bp reads) were performed on llumina NextSeq550  platform.