GSM4252039: infected coral larvae, 3h_S2; Cladocopium goreaui; RNA-Seq
Larvae were removed from FSW, flash frozen in liquid Nitrogen and lysed twice for 20s at 4.0 m s−1 in Lysing Matrix D tubes (MP Biomedicals, Australia) containing 960 µL of lysis/binding solution plus 80 µL of the Plant RNA Isolation Aid (Ambion, USA) on a FastPrep®-24 Instrument (MP Biomedicals, Australia). RNA was bound to the columns, washed multiple times, eluted in 40 μL of RNase-free water and stored at -80°C . Illumina TruSeq RNA Sample Prep Kit was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM4252039