HiSeq Illumina sequencing of granular Anammox culture
DNA was extracted with a PowerSoil DNA Extraction Kit (MoBio). Library was prepared using Nextera DNA Sample preparation kit (Illumina). 50 ng of DNA underwent fragmentation and addition of adapter sequences. Adapter sequences were utilized during a limited cycle PCR (5 cycles). Final library concentration (7.54 ng/uL) with an average library size of 1047 bp was diluted to 10.0 pM and sequenced paired end for 300 cycles using the HiSeq system (Illumina).