Buckeye Lake, Seds Pore Water
Total DNA was submitted to the University of Minnesota Genomics Center (UMGC) where amplicons were sequenced using MiSeq Illumina 2___300_bp chemistry with the primers 515Ff and 806rB targeting V4 hypervariable region of bacterial and archaeal 16S SSU rRNA gene sequences (Caporaso et al., 2012; Apprill et al., 2015) and the primers E572F and E1009 targeting the V9 hyperbariable regiod of eukaryotic 18S SSU rRNA gene seqeunces (Comeau et al., 2011). Each sample was sequenced once. Mothur (ver. 1.40.4; (Schloss et al., 2009)) was used to for post sequencing processing following the MiSeq SOP (Kozich et_al., 2013). Read pairs were assembled and resulting contigs with ambiguous bases were removed. Contigs were trimmed to include only the overlapping regions and unique sequences were aligned against the Silva v132 database. Chimeras were identified and removed using UCHIME (Edgar et al., 2011) and removed.