PacBio SMRT sequence
High molecular weight genomic DNA was sheared into fragments of ~30 kb using a Covaris ultrasonicators (Covaris, Woburn, MA, USA). The fragments were enzymatically repaired and converted into SMRTbellTM template library following the manufacturers instructions. Size-selection was performed to enrich the DNA fragments longer than 10 kb for sequencing on a Pacific Biosciences (PacBio) Sequel Single Molecule Real Time (SMRT) platform.