exome sequencing of affected mouse with spontaneous mutation causing neuromuscular disease
Genomic DNA from an affected mouse was isolated and fragmented. Pre-capture libraries were constructed (Hodges et al., 2009; PMID: 19478811), with 9 cycles of PCR. The product was hybridized to the alpha version of the Roche NimbleGen Mouse Exome capture probe set (Roche NimbleGen, Madison, WI, USA). The sequencing library was quantified by QPCR.