16S of water metagenome: S.J.R polyculture pond
The V3-V4 regions of 16S rRNA genes for each sample were amplified using primers 341F-806R. After purification and quantification, the PCR products from each sample were mixed in equal amounts based on their concentrations, and the amplicon libraries were generated using TruSeq Nano DNA LT Library Prep Kit (Illumina, USA) according to the manufacturers instructions.