Quercus mongolica Fisch. Ex Ledeb. shotgun genomic sequencing; 20 pooled DNAs
Aliquots of genomic DNA from 20 individual trees (~0.1 g each) were pooled and sheared to a median length of ~300 bp and converted into sequencing libraries using Illumina TruSeq v.2 kits at GATC Biotech AG (Konstanz, Germany; pooled samples). Sequencing was performed GATC Biotech AG using the Illumina MiSeq with 2x300 bp paired-end reads.