GSM3043263: VWN2; Homo sapiens; RNA-Seq
The RNA content from each fraction was extracted using TRIzol, following the manufacturer's guidelines. All RNA samples were DNase-treated (TurboDNase, Ambion), phenol/chlorophorm extracted, ethanol precipitated, and resuspended in RNases-free water (Sigma). Fractionations were assessed by western blot analysis, using histone H3, as a chromatin marker, Alyref or Ssrp1 as nuclear markers, and Tubulin as a cytoplasmic marker. Libraries for sequencing were prepared using the Lexogen SENSE mRNA-Seq Library Prep according to the manufacturer's instructions and sequenced using a Hiseq 2500.
GEO Accession
GSM3043263