WGS of Zonotrichia albicollis: adult female liver (tan morph, rep2)
Whole genome sequencing libraries were generated from DNA extracted from the white-throated sparrow livers using a QIAGEN DNeasy Blood and Tissue DNA kit. For each sample, 500ng-1g of DNA was extracted and sheared on a Covaris ultrasonicator to 200-600bp at the Emory Integrated Genomics Core. The DNA fragment ends were repaired, and A-overhangs were added before Nextera barcode adaptors were ligated to the DNA fragments overnight. Finally, the libraries were PCR-amplified to increase concentration and enrich for adaptor-ligated DNA fragments. WGS libraries were sequenced using Illumina HiSeq X Ten with 150 x 2 bp paired-end reads at Macrogen Clinical Laboratory.