GSM4551599: CHO1R; Homo sapiens; RNA-Seq
Colon tissues were collected in ice cold RPMI 1640 (Corning Inc.) and processed within one hour after termination of the surgery Epithelial cells were dissociated by incubating the biopsies in an EDTA-enriched dissociation medium After digestion, the cell suspension was filtered through a 70mm cell strainer, washed in DBPS / 2% FCS / 1mM EDTA and spun down at 400 g for 10 min. After red blood cell lysis (BioLegend), dead cells were depleted using the dead cell depletion kit (Miltenyi Biotec, Germany), following manufacturer's recommendations Single cells were processed through the 10X Chromium Single Cell Platform using the Chromium Single Cell 3′ Library and Gel Bead Kit v2 (10X Genomics, PN-120237) and the Chromium Single Cell A Chip Kit (10X Genomics, PN-120236) as per the manufacturer's protocol Libraries were sequenced on an Illumina NextSeq 500
GEO Accession
GSM4551599