WGBS of Zonotrichia albicollis: adult female hypothalamus (white morph, rep3)
WGBS libraries were prepared using a custom protocol. First, DNA was extracted from the hypothalamus samples using a QIAGEN DNeasy Blood and Tissue DNA kit. For each sample, 100 ng - 1 g of DNA was pooled with 1-5% lambda phage DNA to test for bisulfite conversion efficiency. The DNA samples were then sheared on a Covaris ultrasonicator to 200-600bp. The DNA fragment ends were repaired, and A-overhangs were added before bisulfite compatible adaptors were ligated to the DNA fragments overnight. Then, the DNA fragments were bisulfite-converted and PCR-amplified to increase concentration and enrich for adaptor-ligated DNA fragments. WGBS libraries were then sequenced using Illumina HiSeq X Ten at Macrogen Clinical Laboratory.