Plasmodium falciparum MAINE-Seq Gametocyte
Parasites were extracted from erythrocytes by saponin-lysis and chromatin was digested by MNase for 5 min at 37*C. Sequence libraries were prepared using the BIOO Scientific Library Preparation Kit according to the manufacturer’s instructions, with the exception of the use of the KAPA HiFi Hotstart Ready Mix for the amplification of the libraries. Depending on the amount of input DNA, libraries were amplified for a total of 15 PCR cycles (5 cycles of [15s at 98*C, 30s at 55*C, 30s at 62*C] followed by 10 cycles of [15s at 98*C, 30s at 63*C, 30s at 72*C]).