GBS of Pinus tabuliformis
Genomic DNA was digested by using PstI. Restriction enzyme digestion and adapter ligation were carried out simultaneously on 200 ng DNA from each sample. The digested and ligated DNA was then pooled, purified and PCR amplified. The PCR products were separated on 1.8% high-resolution agarose gels , and fragments within the size range 400 - 500bp were recovered.