GSM4648587: Primary interlobular cells_2; Homo sapiens; RNA-Seq
Total RNA was extracted using Trizol (Thermo Fischer) and a spin column method according to the manufacturer's instructions (Zymo Research) DNAse treated total RNA samples were enriched for mRNAs using oligo dT magnetic beads. mRNAs were fragmented into 200 bp-size fragments and the first strands of cDNAs were synthesized by using random-hexamers. In order to generate the library products, after the second strand synthesis, double strands cDNAs were purified by magnetic beads followed by addition of adenine to the 3'ends and ligation of sequencing adaptors to the fragments. These fragments were amplified by PCR using ABI StepOnePlus Real-Time PCR System, and the quantity and quality was checked by Agilent 2100 Bioanalyzer. Sequencing was performed using BGISeq 500
GEO Accession
GSM4648587