GSM3891612: Bleo1_GFPp; Mus musculus; RNA-Seq
Lung tissues were minced into 1 x 1 x 1 mm pieces and digested with protease solution (Collagenase A, Dispase II, DNase I in RPMI with 10 mM HEPES) at 37°C for 60 min with trituration every 20 min. Cells were stained with antibodies. For murine samples, Col-GFP+ or Col-GFP- cells were sorted. For human samples, lineage (CD31, CD45, CD235a, EPCAM)- cells, or all cells (CD235a-) were sorted. Approximately 10,000 sorted cells were loaded onto 10x Genomics Chromium controller. Single cell libraries were generated using Chromium Single Cell 3' v2 Reagent Kit (10x Genomics) according to the manufacturer's protocol. The libraries were sequenced on Illumina HiSeq 4000 for murine samples or NovaSeq 6000 for human samples.
GEO Accession
GSM3891612