NextSeq 500 sequencing; Single-nucleotide resolution mapping of DNA abasic sites by snAP-seq
Single-nucleotide resolution mapping of DNA abasic sites by snAP-seq
Synthetic DNA was generated by primer extension using template oligos (see methods section for sequences) Input libraries were prepared for each enriched (snAP-seq) library. DNA was end-prepped using an NEBNext Ultra II kit, followed by adapter ligation using Truseq LT indexed adapters (Illumina). PCR amplification conditions were kept consistent with corresponding enriched (snAP-seq) libraries.
Experimental Factor: RNA interference
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Experimental Factor: stimulus
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