GSM2792569: 489_113; Bos taurus; RNA-Seq
At the desired time point the cells were lysed with 2.5 ml Trizol reagent (Invitrogen) for 20 minutes, to ensure mycobacterial inactivation.  RNA was extracted from the samples following the manufacturer's instructions. mRNA was purified from the total RNA and fractionated to generate fragments with a medium size of 180-210 nucleotides.  These fragments were reverse transcribed using random primers to generate cDNA libraries.
GEO Accession
GSM2792569