GSM4210970: WT_cerevisiae_standard-1; Saccharomyces cerevisiae; MNase-Seq
cells were crosslinked with 1% formaldehyde for 15 min (10min for N. crassa), spheroplasted,  digested with micrococcal nuclease and quenched. For "standard" protocol, crosslinks reversed overnight at 65 degrees with proteinase K treatment, phenol-chloroform extracted, RNase-treated, mononucleosome band gel-extracted and CIP-treated. For Rapid protocol, RNase was added for 30 minutes, then crosslinks were reversed at 65 degrees with proteinase K treatment for 45 minutes. DNA was recovered by MinElute column (eluted into 1x cutsmart buffer) and incubated with Quick CIP for 10 minutes. CIP-treated nucleosome-sized DNA footprints were converted to libraries with NuGEN Ovation Ultralow kit
GEO Accession
GSM4210970