vB_VnaS-AQKL99
Three liters of surface seawater from Bayboro Harbor (Tampa Bay, Florida,USA),  prefiltered through a 0.8 m filter ,and viruses were concentrated using iron chloride flocculation. The isolation and plaque purification of the novel phage  were done on a double-layer plaque assay on Vibrio natriegens 14048 on TSB-1 media. Phage DNA was isolated from a 0.2 m-filtered overnight lysate with the Qiagen MinElute Virus Kit