GSM4210284: ORR_OvarianFollicleCell_4C_Replicate1; Drosophila melanogaster; RNA-Seq
Follicle cells were isolated by trypsinizing ovaries from OregonR or Rif1^1/Rif1^2 adult females as described in (Cayirliogu et al., MCB 2003). Follicle cells were FACS sorted into TRIzol LS (Invitrogen) based on their ploidy and RNA was extracted according to the manufacturer's recommendation. 250,000 – 500,000 follicle cells were used per replicate. rRNA was depleted using the RiboMinus™ Eukaryote Kit for RNA-Seq (Invitrogen) and libraries were prepared using the NEBNext® Ultra™ II RNA Library Prep Kit.
GEO Accession
GSM4210284