GSM4211197: ΔelyC rep1_21°C; Escherichia coli; RNA-Seq
Total RNA was purified using the RNeasy Mini Kit (QIAGEN) with the RNAprotect Bacteria reagent (QIAGEN) according to the manufacturer's protocol. DNA was removed using RNase-Free DNase set from QIAGEN. The RNA was finally resuspended in RNase-Free Water (QIAGEN) and RNA concentration was quantified using the NanoDrop ND-1000 at 260 nm. RNA Ribosome Integrity Numbers (RIN) and purity of the RNA samples were assessed using the Agilent Bioanalyzer 2100 and RNA 6000 Pico Kit. The RIN numbers for all the twelve samples were 9.3 to 10. The rRNA depletion was performed using the Ribo-Zero Magnetic Gold Kit for Gram-negative Bacteria (Illumina). RNAseq libraries were prepared using KAPA RNA stranded Kit (Roche-Nimblegen). Libraries were assessed and quantified by BioAnalyzer using the High Sensitivity DNA Kit (Agilent). Sequencing was performed on an Illumina HiSeq2000 machine, in 75-nucleotide paired-end mode.
GEO Accession
GSM4211197