RNA-Seq of Elaphe carinata:  three months SZMMB8 muscle
TotalRNAwasextractedusingTrizolreagent(Invitrogen,CA,USA)followingthemanufacturer'sprocedure.ThetotalRNAquantityandpuritywereanalysisofBioanalyzer2100andRNA1000NanoLabChipKit(Agilent,CA,USA)withRINnumber>7.0.Poly(A)RNAispurifiedfromtotalRNA(5ug)usingpoly-Toligo-attachedmagneticbeadsusingtworoundsofpurification.Followingpurification,themRNAisfragmentedintosmallpiecesusingdivalentcationsunderelevatedtemperature.ThenthecleavedRNAfragmentswerereverse-transcribedtocreatethefinalcDNAlibraryinaccordancewiththeprotocolforthemRNASeqsamplepreparationkit(Illumina,SanDiego,USA),theaverageinsertsizeforthepaired-endlibrarieswas300bp(50bp).Andthenweperformedthepaired-endsequencingonanIlluminaHiseq4000atthe(LCSceiences,USA)followingthevendorsrecommendedprotocol.