Pooled sample consisting of samples: Cyst 857 S1|Cyst 858 S1|Cyst 859 S1|Cyst 867 S1|Cyst 875 S1|Cyst 884 S1|Cyst 813 S1|Cyst 853 S1|Cyst 838 S1|Cyst 846 S1|Cyst 886 S1|Cyst 815 S1|Cyst 839 S1|Cyst 847 S1|Cyst 816 S1|Cyst 840 S1|Cyst 856 S1|Cyst 888 S1
Primers were used to amplify DNA in 25-uL multiplex polymerase chain reactions (PCRs) as described previously 15.  For each sample, three multiplex PCRs were performed, with each multiplex PCR containing 22 to 50 primer pairs.  Reactions were purified with AMPure XP beads (Beckman Coulter) and eluted in 100 uL of Buffer EB (Qiagen, cat. no. 19086).  0.25% of the purified PCR products were then amplified in a second round of PCR, as described in Springer et al.15.  The PCR products were purified with AMPure and used for sequencing on a MiSeq or HiSeq instrument