Mycobacterium bovis BCG-1 (Russia) comparative study
1 ?g of amplified DNA was fragmented to 150300 bp by sonication with the Covaris S220 System (Covaris, Woburn, Massachusetts, USA). The Ion Xpress  Plus Fragment Library Kit (Life Technologies) was then used to prepare a barcoded shotgun library. The PCR Ion PGM  Template OT2 200 Kit (Life Technologies) was used for emulsion PCR.