RNA-Seq of AMH-treated (Anti-Mllerian Hormone) mus musculus AT29C  ovarian cell line.
All RNA sequencing libraries were constructed from 1 g of  total RNA using Illumina TruSeq RNA Sample Prep (Illumina) according to the  manufacturers instructions. The libraries were quantified using the qPCR NGS  Library Quantification Kit (Agilent). Each cDNA library was tagged with a  specific Illumina adapter sequence and the libraries were subsequently pooled  and loaded on two sequencing lanes. Sequencing was performed on an Illumina  HiSeq 3000 apparatus using the Illumina TruSeq Standard mRNA kit v2 to obtain  paired-end reads (2 x 150 bp).