GSM1184662: ERF115-HBH ChIP output; Arabidopsis thaliana; ChIP-Seq
Cells were grinded in N2(l), mixed with extraction buffer and incubated with detergents for 30 minutes. After sonication, the extract was clarified by centrifugation.  ERF115-DNA complexes were isolated through a Tandem Chromatin Affinity Purification procedure (TChAP) with Ni2+-NTA and Streptavidin-sepharose beads based on the HBH tag. Standard Illmuna Protocol
GEO Accession
GSM1184662