Pooled sample consisting of samples: Cyst 308 S1|Cyst 316 S1|Cyst 326 S1|Cyst 327 S1|Cyst 331 S1|Cyst 333 S1|Cyst 334 S1|Cyst 336 S1|Cyst 351 S1|Cyst 352 S1|Cyst 354 S1|Cyst 357 S1|Cyst 358 S1|Cyst 359 S1|Cyst 360 S1|Cyst 371 S1|Cyst 374 S1
Primers were used to amplify DNA in 25-uL multiplex polymerase chain reactions (PCRs) as described previously 15.  For each sample, three multiplex PCRs were performed, with each multiplex PCR containing 22 to 50 primer pairs.  Reactions were purified with AMPure XP beads (Beckman Coulter) and eluted in 100 uL of Buffer EB (Qiagen, cat. no. 19086).  0.25% of the purified PCR products were then amplified in a second round of PCR, as described in Springer et al.15.  The PCR products were purified with AMPure and used for sequencing on a MiSeq or HiSeq instrument