Pooled sample consisting of samples: Cyst 315 S1|Cyst 317 S1|Cyst 320 S1|Cyst 324 S1|Cyst 328 S1|Cyst 329 S1|Cyst 335 S1|Cyst 338 S1|Cyst 339 S1|Cyst 342 S1|Cyst 344 S1|Cyst 345 S1|Cyst 349 S1|Cyst 363 S1|Cyst 364 S1|Cyst 369 S1|Cyst 370 S1|Cyst 376 S1|Cyst 378 S1
Primers were used to amplify DNA in 25-uL multiplex polymerase chain reactions (PCRs) as described previously 15.  For each sample, three multiplex PCRs were performed, with each multiplex PCR containing 22 to 50 primer pairs.  Reactions were purified with AMPure XP beads (Beckman Coulter) and eluted in 100 uL of Buffer EB (Qiagen, cat. no. 19086).  0.25% of the purified PCR products were then amplified in a second round of PCR, as described in Springer et al.15.  The PCR products were purified with AMPure and used for sequencing on a MiSeq or HiSeq instrument