GSM4211213: BT483_5_siNLK_type1_Tam; Homo sapiens; RNA-Seq
Total RNA was extracted from cells in individual confluent 10 cm dish, using the standard procedure of Qiagen RNeasy kit. The primary quantitation and degradation or the extracted RNA were checked by nanodrop and agrose gel electrophoresis. The integrity and purity of RNA were examined by Agilent 2100. The NovaSeq 6000 library for DNA sequencing was prepared using TruSeq Stranded mRNA Library Prep Kit (Illumina) following the protocol provided by the manufacturer. The final libraries were normalized by quantification with LightCycler 480 II (Roche Applied Science, Indianapolis, IN, USA) and qualification with Bioanalyzer (Agilent, Palo Alto, CA, USA).
GEO Accession
GSM4211213