PMS-122
"To detect variation and population structure in a broad sample of Miscanthus sinensis, genotyping-by-sequencing was performed with the following protocol:  DNA was digested with PstI and MspI and ligated to barcoded PstI adapters and a universal MspI adapter.  95 samples were pooled to create one library.  Size selection of 200-500 bp was performed on a 2% agarose gel.  After sequencing, the software Stacks was used to place reads into separate files by barcode, remove barcode sequence, trim the remaining sequence to 80 nt, and filter out low quality reads and reads with uncalled bases."