Raw Transcriptome NGS GAII data for Orchesella cincta
1.5 *g of normalized cDNA in 100*l 10mM Tris/HCl was fragmented. After fragmentation, end repair, ligation of adapters, and enrichment of the fragments was done using the Paired-End Sequencing kit of Illimina*, according to the manufacturer’s protocol. All purifying steps were done using the MinElute PCR purification kit (Qiagen*). Quality and concentration of the fragmented and enriched library was verified on a lab-on-a-chip (Agilent Technologies). Next-generation sequencing of the 180 – 200 bp fragments for O. cincta was performed on the Next Genome Analyzer II platform.