Illumina HiSeq 2000 paired end sequencing; Exomes of human leukemic JMML (Juvenile MyeloMonocytic Leukemia) xenografts in NSG or NSG-S mice (samples enriched in hCD45)
Exomes of human leukemic JMML (Juvenile MyeloMonocytic Leukemia) xenografts in NSG or NSG-S mice (samples enriched in hCD45)
humanCD45 enriched xenograft samples from NSG or NSG-S xentransplanted mice with JMML CD34+ cells. For secondary xenografts, bone marrow cells from primary engrafted mice were injected in another mouse to test the self-renewal capacities of JMML cells. The bone marrow cells of the primary mice were not sorted before the injection into the secondary recipient because the percentage of human CD45 was high. Qiagen Mini or Midi Kit (Qiagen Gmbh) Exome capture was carried out using the SureSelect Human All Exon V5 or SureSelect Clinical Research (Agilent Technologies, Santa Clara, CA, USA) according to manufacturer's instruction and protocols.
Experimental Factor: disease staging
not applicable
Experimental Factor: individual
NSG mouse 2
Experimental Factor: xenograft mouse strain
not applicable
Experimental Factor: sampling time point
not applicable