16S rRNA seq of Gadus morhua microbiome: Intestinal sample
Intestinal content and mucus lining was homogenized and DNA                 extracted with QIAamp DNA Stool Mini Kit from Qiagen. Primers U786F and A1067R                 covering the V5/V6 variable region of the 16S rRNA were used. Amplicon fusion                 primers were designed for 454 sequencing (Roche). Following PCR amplification and                 verification on agarose gels, the PCR products were purified with the Agencourt                 AMPure XP PCR purification system to eliminate primer dimers. DNA concentrations                 were measured and PCR products pooled for sequencing.