Membrane B 16S region 3 replicate
PCR products were purified and Miseq (Illumina) were prepared according to manufactureer s instructions. DNA libraries were checked for size, concentration and integrity using a Bioanalyzer (Agilent). Amplicon sequencing was performed using an Illumina Miseq sequencer. Paired -end reads (2x300) were generated according to manufacturer s instructions obtaining at least 100000 reads per replicate.