GSM3405625: Log cells; Saccharomyces cerevisiae; OTHER
Spheroplasts were incubated with a concentration of micrococcal nuclease titrated to each experiment designed to retain ~90% mononucleosome sized fragments in the insoluble chromatin fraction for 20 min at 37 degrees. DNA ends were repaired and biotin labeled, fragments were proximity ligated, crosslinking was reversed, and DNA was purified by phenol-chloroform extraction and ethanol precipitation. Purified DNA ends were readied for ligation, A-tailed, and Illumina Truseq adapters were ligated.  Proximity ligated DNA was pulled down with streptavidin beads, and library was amplified with Illumina Truseq primers.
GEO Accession
GSM3405625