GSM2527895: empty vector-no Ab replicate 2; Proteus mirabilis; ChIP-Seq
Formaldehyde-crosslinked frozen pellets were thawed on ice followed by cell lysis in 8 ml of ChIP lysis buffer (1% Triton X-100, 1mM EDTA, 15mM Tris-Cl pH 7.5, 150mM NaCl) that was fortified with protease inhibitor cocktail (Thermo Fisher). Cells were broken by French press (3 passes) followed by centrifugation of 800 ul lysate aliquots at 17115 x g for 10 minutes to get rid of cellular debris. Each 800ul clean lysate corresponds to 10 ml of cell pellet. As in Singh et al, 2014 Genes & Development doi: 10.1101/gad.234336.113
GEO Accession
GSM2527895