GSM4387139: Granulosa cells_control_Replicate_1; Bos taurus; miRNA-Seq
miRNA enriched total RNA was extracted using miRNeasy Mini Kit (Qiagen GmbH, Hilden, Germany The library preparation was done using the QIAseq miRNA Library Kit (QIAGEN). A total of 100ng  total RNA was converted into miRNA NGS libraries. Adapters containing UMIs were ligated to the  RNA. Then RNA was converted to cDNA. The cDNA was amplified using PCR (16 cycles) and  during the PCR indices were added. After PCR the samples were purified. Library preparation QC  was performed using either Bioanalyzer 2100 (Agilent) or TapeStation 4200 (Agilent).  Based on quality of the inserts and the concentration measurements the libraries were pooled in  equimolar ratios. The library pool(s) were quantified using qPCR. The library pool were then  sequenced on a NextSeq550 sequencing instrument according to the manufacturer instructions. Raw  data was de-multiplexed and FASTQ files for each sample were generated using the bcl2fastq  software (Illumina inc.). FASTQ data were checked using the FastQC tool.
GEO Accession
GSM4387139