RNA-Seq of Malus domestica cv. Fuji
Total RNA from fruits of both treatment and the control were pooled prior to library preparation. Prior to cDNA library construction, poly-T oligo-attached magnetic beads were used to purify the mRNA, which was added to fragmentation buffer to break mRNA into short segments of approximately 200 bp followed by cDNA synthesis using random hexamers and reverse transcriptase. Then added buffer, dNTPs, RNAse H and DNA polymerase I to synthesize the second strand cDNA.  Both ends of sequencing adapters were ligated after terminal repairing of double stranded cDNA fragments. The AMPure XP system (Beckman Coulter, Beverly, USA) was used to purify the final cDNA library and selectively enriched by PCR enrichment. The library preparations were sequenced by Novogene Bioinformatics Technology Co., Ltd (Beijing, China).