18S rRNA amplicon sequencing of soil sample DNA: New Zealand
18S rRNA gene libraries were prepared using eukaryotic primers Euk1391f/EukBr targeting the V9 region of the 18S rRNA gene. Library preparation and sequencing were conducted according to the standard protocol (Version 4_13) of the Earth Microbiome Project and libraries were paired-end sequenced using the Illumina Miseq platform.