Bison_x_Simmental F1 Oxford Nanopore
Template for long read sequencing on the PromethION instrument was prepared using the Ligation Sequencing Kit LSK-109 (Oxford Nanopore Technologies, Oxford, U.K.).  Briefly, 10 ug of DNA was sheared by passing 5 times through a 26 gauge needle and concentrated by addition of 1 volume of AMPureXP bead solution.  After collecting the beads by magnet, the beads were washed 2 times with 0.5 mls 80% ethanol and dried for 2 minutes.  The beads were suspended in 51 uL EB and DNA eluted at 37C for 5 minutes.  The sheared, eluted DNA was then processed by ligation to AMX adapter as recommended by the manufacturers instructions in the LSK-109 product manual and sequenced on a PromethION instrument using R9.4.1 flow cells.