Diversity, Distribution and Hydrocarbon Biodegradation Capabilities of Microbial Communities in Oil-Contaminated Cyanobacterial Mats from a Constructed Wetland
Purified DNA extracts were submitted to Molecular Research MR DNA Laboratory (Shallowater, TX, USA) for tag-pyrosequencing. Bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP) was performed as described before [24,25] using the GS FLX titanium sequencing kit XLR70. One-step PCR was performed using a mixture of hot start and hot start high fidelity taq polymerases resulting in amplicons that extend 350-450 bp from the 27F region (E. coli rRNA numbering). The bTEFAP sequencing was performed according to the MR DNA protocols (www.mrdnalab.com).