FLU4-752
B cells were sorted from PBMC. The following definitions were used for various populations and can be identified within the sequence set title.   Sample codes: SLE = systemic lupus erythematosus (all except timecourse studies were during acute flare) FLU = 6-7 days post influenza vaccination TET = 6-7 days post tetanus vaccination IGG = IgG4RD (sample 1-267 had a local response and 2-337 had a systemic response) PEM = Pemphigus  Naive (or N): CD19+IgD+CD27- Activated Naive (or AN, AcN): CD19+IgD+CD27-CD24-MTG+ Resting Naive (or RN, TN): CD19+IgD+CD27-MTG- IgD- Memory (or M, SM, SwM): CD19+IgD-CD27+(excludes CD27hiCD38hi) CD138- ASC (or PB): CD19+/-IgD-CD27hiCD38hiCD138- CD138+ ASC (or PC): CD19+/-IgD-CD27hiCD38hiCD138+  Timecourse sets are labeled by MMDD (or MDD) at the beginning of title. (eg. 0403 = April 3)  For further methods and cell numbers see Tipton et al. "Diversity, Cellular Origin, and Autoreactivity of ASC in acute SLE" Nat Immunol. 2015