16S rRNA V4 from coral reef sediment (SampleID: O_SD28): With peptide nucleic acid clamp
Microbial DNA extracted from 0.25g of reef sediment. DNA extracted using Qiagen PowerSoil kit following manufactuer protocols. Amplified using 515F-806R primers for 16S rRNA (V4 region). PNA clamp designed in Reigel et al. 2020 was added to the PCR reaction.