NextSeq 500 paired end sequencing; Evidence of developmental escape from transcriptional-gene-silencing in MESSI retrotransposons
Evidence of developmental escape from transcriptional-gene-silencing in MESSI retrotransposons
Samples were harvested at the end of the day, and consisted of pools of at least a hundred whole shoot apical meristems or at least six first leaves. Samples were collected from two week old plants of tomato. S. lycopersicum seeds were directly sown in soil (Levington F2, ICL) and stratified in the dark for one week at 4°C. Plants were grown in a Conviron MTPS chamber (Conviron) at 25/18°C (day/night); with 16/8 hours light cycle, 300 µmol/m2/s light intensity and ~60% humidity. DNA was extracted with the DNeasy Plant Mini Kit (Qiagen). PCR-free libraries for the DNA-seq were prepared with 1 ug of DNA using the TruSeq DNA PCR-Free Library Prep kit (Illumina); following provider's instructions.
Experimental Factor: organism part
leaves