GSM4254372: MM2_01-24-17 (WT); Mus musculus; RNA-Seq
Cells were scraped in TRIzol reagent total RNA was isolated using phenol-chloroform extraction using Invitrogen PureLink RNA Mini Kit (#12183018A) RNA sequencing libraries were prepared using the TruSeq Stranded Total RNA with Ribo-Zero Gold kit (Illumina). This protocol provides strand specific data while Simultaneously removing both cytoplasmic and mitochondrial rRNA. Libraries were sequenced on the HiSeq 2500 instruments and 50 million 50 base pair single end Reads were produced per sample. Data was extracted, de-convoluted based on dual index reads and analyzed using CLC Genomics Workbench software (Qiagen).
GEO Accession
GSM4254372