MinIon sequencing of Wolbachia-infected Anastrepha striata, and Spiroplasma (strain sHy2)-infected Drosophila hydei
For this library, we combined samples from two different sources. The predominant source was DNA isolated with a phenol-chloroform method from 3 individuals (with heads removed) of Anastrepha striata infected with Wolbachia. The other source (which was "spiked in") was DNA isolated with a chloroform-ethanol protocol from a very small volume of hemolymph that had been passed through a 70µm filter, obtained from Drosophila hydei (isoline H25) infected with Spiroplasma strain sHy2.