GSM3782928: U87_ PELP1 siRNA_1; Homo sapiens; RNA-Seq
U87 cells transfected with control siRNA or PELP1 siRNA using oligofectamine transfection reagent and after 72 hours total RNA was isolated using RNeasy mini kit using manufacturer's protocol (Qiagen, Valencia, CA). Total RNA samples about 1-2 micro grams were used for sequencing library preparation according to Illumina TruSeq Total RNA Sample Preparation Guide (Illumina Cat. #: RS-122-2201). Each library was bar-coded and then pooled for cluster generation and sequencing run with 50bp single-end (SE) sequencing protocol.
GEO Accession
GSM3782928