GSM3785161: SM-HCP (ZA-ZD); Homo sapiens; RNA-Seq
RNA will be extracted from the FFPE sample using Qiagen's AllPrep FFPE kit.  The sample will be added to lysis buffer containing proteinase and then centrifuged to create a pellet and a supernatant.  The RNA will be purified from the Supernatant. RNA is quantified via RiboGreen and RNA quality is measured by the DV200 score (fraction of RNA fragments whose length is > 200nts). Due to the inherent nature of samples derived from FFPE to contain degraded and cross-linked nucleic acids, all FFPE samples will be accepted into the workflows at risk and will be billed accordingly. RNA will be processed for Transcriptome Capture. Library preparation utilizes a stranded cDNA library from isolated RNA, then hybridizes the library to a set of DNA oligonucleotide probes to enrich the library for coding region transcript fragments. Transcriptome Capture targets 21,415 genes, representing 98.3% of the RefSeq exome. Due to the inherent nature of samples derived from FFPE to contain degraded and cross-linked nucleic acids, all FFPE samples will be accepted into the workflows at risk and will be billed accordingly.
GEO Accession
GSM3785161