GSM3662062: ChIP_ClsN_IP_Sac_sta_rep2; Sulfolobus acidocaldarius; ChIP-Seq
Crosslinked cells were lysed by sonication, and the extract was clarified by centrifugation. Protein-DNA complexes were purified from the extract with antisera. After reversal of crosslinks, ChIP DNA and input DNA were extracted with phenol:chloroform:isoamyl alcohol and then precipitated in ethanol. Libraries were constructed using Illumina TruSeq ChIP Library Preparation Kits and were sequenced on the Illumina NextSeq 500 platform.
GEO Accession
GSM3662062