GSM4255680: NEU 1157; Mus musculus; RNA-Seq
After Ficoll separation of peripheral blood, neutrophils were collected from the cell pellet following red cell lysis  Total RNA was extracted using TRIzol and the Ambion PureLink RNA Mini Kit (Life Technologies, cat# 12183018), treated with DNase to remove genomic DNA using the DNAfree Kit (Life Technologies, cat# AM1906). Sequencing libraries were constructed from 100 ng of DNase-treated, total RNA using the ScriptSeqv2 Complete Kit (Illumina cat# BHMR1224). The RNA depleted for rRNA using the Ribo-Zero removal reagents and fragmented. First strand cDNA was then synthesized using a 5' tagged random hexamer and reverse transcription, followed by annealing of a 5' tagged, 3'-end blocked terminal-tagged oligo and second strand synthesis. The Di-tagged cDNA fragments were purified, barcoded, and PCR-amplified for 15 cycles.
GEO Accession
GSM4255680