Mesocricetus auratus Arenavirus-infected livers- 1-Liver-control
All infections were performed under ABSL-3 conditions. Hamsters were inoculated i.m. with 10^4 TCID50 Pirital strain PIRV VAV-488 in the left hindquarters. Control hamsters were sham-inoculated with sterile PBS. Between 7 and 12 days post inoculation, hamsters began to show signs of disease and were euthanized for organ retrieval. Total RNA was extracted from 30mg of flash frozen liver. Total RNA was extracted, prepared with the Illumina Ribo-Zero RNA-Seq library prep and sequenced as 2x100 paired end reads on an Illumina HiSeq 2000.   Fastq files were quality and adapter-trimmed with using default parameters of Trimmomatic (Lohse et al. 2012).