GSM1350524: Tropicalis_3seq_stage10.5-R1; Xenopus tropicalis; RNA-Seq
Total RNA was extracted with Trizol reagent, followed by mRNA preparation using oligo-DT Dynabeads (Invitrogen, cat #610.06). 400 ng of mRNA was then used to prepare 3' RNA-Seq libraries as previously described (Beck et al, PLOS One 2010). Briefly, mRNA was heat sheared for 7 minutes to produce an average fragment size range of 300-500 bp, then used to generate cDNA libraries using a custom oligo dT primer containing Illumina-compatible adapter sequence. cDNA fragments were end-repaired and ligated to standard Illumina adapters. Size-selection was performed using E-gel SizeSelect agarose gels (Invitrogen), products were PCR amplified for 15 cycles, and purified using Ampure XP beads. Library quality was assessed using Bioanalyzer and Qubit, and sequenced on the Genome Analyzer IIx.
GEO Accession
GSM1350524