Marchantia sporophyte rep2
Marchantia sporophytes were collected thirteen days after crossing and fixed in ice-cold acetone overnight. Fixed samples were processed for laser microdissection as outlined in (Scanlon et al. 2009). Approximately 100,0002  microns of cells were harvested for each biological replicate. RNA was extracted from all collected tissues with the PicoPure RNA Isolation Kit (Life Technologies, catalog number KIT0204) and in vitro amplified using a TargetAmp 2-round aRNA Amplification Kit 2.0 (Epicentre, catalog number TAU2R51224). Each amplified RNA sample was prepared for sequencing following the protocols of (Kumar et al. 2012) with a modified procedure for single sample processing. The libraries were ligated to barcoded adapters and pooled for 8-plex sequencing on an Illumina HiSeq 2000 at the Cornell University CLC DNA sequencing facility (http://cores.lifesciences.cornell.edu/brcinfo/?f=1).