NextSeq 500 paired end sequencing; RNA-Seq of CD19+ B-cell populations from patients with juvenile dermatomyositis and controls
RNA-Seq of CD19+ B-cell populations from patients with juvenile dermatomyositis and controls
CD19+ B cells were sorted from freeze-thawed peripheral blood mononuclear cells (PBMC) by flow cytometry (FACS Aria III), using CD19 PE-Cy7 (BioLegend). DAPI was used to exclude dead cells. Sorted B cell RNA was extracted using the Arcturus PicoPure RNA Isolation Kit. Samples were processed using Illumina's TruSeq Stranded mRNA LT sample preparation kit (p/n RS-122-2101) according to manufacturer's instructions.
Experimental Factor: normal
disease
Experimental Factor: control
sampling time point