mRNA seq to generate a reference transcriptome of the newt
Total RNA was purified from target tissues conventionally and evaluated by Agilent 2100 Bioanalyzer (Agilent Technology, Santa Clara CA 95051). From this qualified RNA sample, a cDNA library (insert size: 300-400 bp) was constructed according to the instruction provided by Illumina (San Diego, CA 92122). Paired-end sequencing (100 bp read x 2) was carried out by Illumina HiSeq2500 and a set of raw read data was produced after base calling and Chastity filtering (CASAVA ver.1.8.1., Illumina).