GSM4929064: Il17b-/- and WT mice+ DSS-1 mixed sample (non-demultiplexed); Mus musculus; RNA-Seq
The pooled cells were loaded on a BD Rhapsody™ Cartridge for capturing the single cells by pairing one cell with one barcoded beads. Then the cells were lysed to release mRNA, which were captured by barcoded oligo on bead, which has the cell label information and unique molecular identifiers information. Then the beads were retrieved from Cartridge into one tube and performed reverse transcription. The cDNA of captured mRNA was first synthesized on BD Rhapsody™ Cell Capture Beads as described in the instrument user guides. At the same time, the barcode information from BD Rhapsody Cell Capture Beads is also added to Sample Tags during reverse transcription. To generate the Sample Tag sequencing libraries, the extended Sample Tags are first denatured from the BD Rhapsody Cell Capture Beads, which are later amplified through a series of PCR steps. Meanwhile, the whole transcriptome amplification library is generated directly from the BD Rhapsody Cell Capture Beads using a random priming approach, followed by an index PCR step according to BD Rhapsody protocol. Both the whole transcriptome mRNA and Sample Tag library were both sequenced on Illumina nova6000.
GEO Accession
GSM4929064