GSM1195625: sR4; Medicago truncatula; RNA-Seq
Plant roots were harvested and crushed with liquid nitrogen. For the Myc-LCO and Nod factor libraries and their corresponding controls, total RNA were isolated from 100 mg of powder with the mirVana miRNA isolation kit (Ambion) according to the manufacturer’s instructions. For the other libraries, total RNAs were extracted using 1 mL of Trizol per 50-100 mg of powder. Phenol-Chloroform separation, isopropanol precipitation, ethanol wash and RNA solubilization were performed according to the TRI REAGENT manufacturer’s instructions. RNA integrity and quality have been checked thanks to the Agilent Bioanalyzer 2100. The smRNA libraries were obtained thanks to the Small RNA Sample Prep kit from Illumina according to the “Preparing Samples for Small RNA sequencing using the Alternative v1.5” protocol. libraries were sequenced using HiSeq Solexa sequencer from Illumina.
GEO Accession
GSM1195625