freshwater metagenome
Effluent was collected from Manitowoc wastewater treatment plant, filtrate after running through ~1.6 _m-pore-size-glass-fiber filter (pre-filtration) was filtered through 0.22_m-pore-size polycarbonate membrane filter for collecting smaller and free-living microbes. DNA was extracted using phenol:chloroform based extraction, and submitted for WGS sequencing with paired-end format (100bp each) and approximate insert size of 400bp.