Illumina HiSeq 2000 sequencing; Whole genome sequencing of  Mycobacterium tuberculosis strains resistant to D-cycloserine
Whole genome sequencing of  Mycobacterium tuberculosis strains resistant to D-cycloserine
Mycobacterium tuberculosis H37Rv was grown in 7H9 medium supplemented with 10% ADC under constant rotation at 37C. 2 ml of stationary phase cultures of desired strains were harvested by centrifugation, resuspended in 0.2 ml TE buffer and heat-killed at 80 C for 1 h. Genomic DNA was extracted using the Zymo ZR genomic DNA II and genomic DNA clean and concentrator kits, following the manufacturer's instructions. Quality and quantity of genomic DNA was confirmed by agarose gel electrophoresis and spectrophotometry (NanoDrop). Sequencing libraries were constructed using the Epicentre Nextera DNA kit according to manufacturer's instructions
Experimental Factor: individual
DCSr mutant 4
Experimental Factor: phenotype
strain with resistance to D-cycloserine