GSM3893757: USOS-UVSSA-KO-CPD-Xrseq Rep2; Homo sapiens; OTHER
XR-seq was performed as previously described (Hu. et al Genes and Dev. 2016, Adar S. et al PNAS 2016). Briefly, cells were harvested 3h after treatment with 20J/m2 UVC (254nm). Primary excision products were pulled down by TFIIH coimmunoprecipitation with anti-p62 and anti-p89 antibodies (Santa Cruz Biotechnology sc25329 and sc271500), and ligated to both 5′ and 3′ adaptors. Ligation products containing CPD were purified by immunoprecipitation with the anti-CPD antibody (Cosmo Bio NM-DND-001) and repaired in-vitro by Drosophila melanogaster CPD photolyase. Repaired DNA were PCR-amplified with Index primers and purified by 10% native polyacrylamide gels.
GEO Accession
GSM3893757