Chromatin accessibility, Danio rerio adult retinal ganglion  cells, control, replicate 3
Chromatin was  extracted from nuclei isolated from FACS sorted RGCs, enzymatically  dissociated from adult zebrafish retinas. Multiple retinas from the  fgap43:egfp strain were dissciated and sorted for RGCs expressing GFP using a  Becton Dickinson FACSAria III sorter. Although levels of  endogenous Gap43 are low in the nave adult retina, the GFP transgene tends  to be long lived and is detectable by FACS at levels that are above  background but below the level of visual detection. Each  replicate is derived from 50,000 pooled from biologically distinct retinas.  ATAC-seq libraries were prepared using the Tn5 transposase system (Nextera  DNA library kit, Illumina, FC-1211030) as previously described by the  Greenleaf lab and purified using DNA Clean & Concentrator kit (SKU:  ZD5205, Zymo).