GSM1192174: ChIP-seq_NRSF/REST_basal SF-1; Homo sapiens; ChIP-Seq
Lysates were clarified from sonicated nuclei and SF-1 - DNA complexes were isolated with antibody, as described in Lee TI, Johnstone SE, Young RA (2006) Chromatin immunoprecipitation and microarray-based analysis of protein location. Nature Protoc 1(2):729-748. Libraries construction was contracted out to Fasteris SA. At Fasteris SA, barcoded libraries were prepared such that four samples could be run per lane. The quality of starting DNA was assessed prior to library construction at Fasteris SA using an Agilent Bioanalyzer (Agilent, Santa Clara, CA, USA).
GEO Accession
GSM1192174