An improved genome reference of the African cichlid, Metriaclima zebra
The Qiagen MagAttact HMW DNA kit was used to create high-molecular weight DNA from a nucleated blood cell sample. Size selection was performed at the University of Maryland Genomics Resource Center using a Blue Pippin pulse-field gel electrophoresis instrument. A library was constructed and 24 SMRT cells were sequenced on their PacBio RS II using the P5-C3 chemistry.