GSM4703989: Normal Male Gonad; Homo sapiens; RNA-Seq
Cold lysis buffer (0.1 M Tris-HCl pH 7.5, 0.5 M LiCl, 1% SDS, 10 mM EDTA, and 5 mM dithiothreitol) was pipetted over the surface of the plate and removed after 12 min of incubation. The beads were then collected. After reverse transcription, exonuclease I treatment and cDNA amplification, the TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme) was used with 1 ug of total RNA for the construction of sequencing libraries. Library preparations were performed with the Microwell-seq protocol
GEO Accession
GSM4703989