Transcriptome sequencing of kidney in Fejervarya cancrivora
RNA library construction was followed Illumina Hiseq2000 RNA sample preparation kit (Illumina, San Diego, CA). Briefly, mRNA was isolated from 8–10ug total RNA using the polydT beads and then fragmented and reverse transcribed. The fragmented cDNA was end-repaired, added “A” with the 3’ end, and ligated with Illumina adaptors. Ligation products were purified on 2% agarose gel and enriched by a 15-cycle PCR. The libraries were normalized and sequenced on Illumina Hiseq2000 instrument (Illumina, Inc., San Diego, California USA) as 2*101bp.