RNA-seq of Salmo salar: head kidney of fry fed with control diet - individual 3
Total RNA was extracted with an Animal RNeasy mini kit (Qiagen, Hilden, Germany) according to manufacturers instructions. Extraction was followed by an on-column DNase digestion by an RNase-free DNase (Qiagen, Hilden, Germany) and spin RNA clean-up. Sequencing libraries were prepared with a TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, USA) according to the manufacturers protocol and sequenced on a HiSeq 2500 platform (Illumina, San Diego, USA) with a single-end 120 cycles strategy.