GSM3892345: Tfhs hashtag library; Mus musculus; RNA-Seq
Sorted cells from each mouse sample were labeled with cell hashing antibodies (BioLegend TotalSeq anti-mouse Hashtag #3-6). 12,000 cells (4000 cells from each hashtagged mouse) were loaded into one lane of a 10X Chromium microfluidic chip. Single cell capture, barcoding and library preparation were performed using the 10X Chromium platform, version 2 chemistry for the Tfh sort and version 3 chemistry for the IL-4 4Get+ sort. cDNA and libraries were checked for quality on Agilent 4200 Tapestation, quantified by KAPA qPCR, and pooled using a ratio of 95% gene expression library and 5% hashtag library before sequencing, one on a single lane of an Illumina HiSeq4000 and the other on 16.67% of lane of an Illumina NovaSeq 6000 S2 flow cell, both targeting 6,000 barcoded cells with an average sequencing depth of 50,000 reads per cell. Chromium System (10X Genomics) v2 or v3 chemistry.
GEO Accession
GSM3892345