16S rRNA gene seq of C57BL/6 mouse fecal microbiota
Total DNA was extracted from mouse feces using the QiaAmp DNA stool mini kit (Qiagen, #51504). Sequencing libraries of the 16S rRNA gene were prepared according to the Illumina 16S Metagenomic Sequencing Library Preparation Guide Rev. B using a MiSeq instrument and the MiSeq 600-cycle v3 kit (Illumina, MS1023003). For detailed methods, see: Power, K. A., Lepp, D., Zarepoor, L., Monk, J. M., et al., Dietary flaxseed modulates the colonic microenvironment in healthy C57Bl/6 male mice which may alter susceptibility to gut-associated diseases. J. Nutr. Biochem. 2016, 28, 61–69.