GSM4256026: SE20P2Cet2; Mus musculus; RNA-Seq
Three tumors from each group (vehicle and cetuximab, a total of 6 samples for each PDX) were subjected to sequencing. Tumors were obtained from different mice within the group. RNA was extracted using the RNeasy mini kit (Qiagen) according to the manufacturer's instructions. After quality control of Bioanalyzer (RIN>7), RNAseq libraries were prepared using the TruSeq RNA Sample Preparation kit (Illumina) according to the manufacturer's protocol. Briefly, 1ug of total RNA was fragmented, followed by reverse transcription and second-strand cDNA synthesis. The DS-cDNA was subjected to end repair, a base addition, adapter ligation, and PCR amplification to create libraries. Qubit and Bioanalyzer were evaluated. Sequencing libraries were constructed with barcodes to allow multiplexing of 6 samples in one lane. Libraries, ready to run, were sent to the g-INCPM institute at the Weizmann Institute of Science for sequencing. On average, 400 million paired-end (75 bp) reads were sequenced per run on an Illumina NextSeq 500 HO instrument.
GEO Accession
GSM4256026