GSM3721959: anp1_TONO_10dai; Lotus japonicus; RNA-Seq
Nodule primordias and developing nodules were excised from roots. Total RNA was isolated using Cetyltrimethylammonium Bromide (CTAB) and LiCl. Total RNA was treated with DNase (Qiagen) and Ribo-Zero kit for plant seed/root and for gram-negative bacteria (Epicentre) to remove genomic and ribosomal DNA. 50 ng of RNA was used for library preparation. RNA libraries were prepared using Scriptseq Ver2 library preparation kit (Epicentre) by following manufacture's instructions
GEO Accession
GSM3721959