GSM2527234: Microdissected fresh tip individual 2; Homo sapiens; RNA-Seq
Fresh tissue was mmicrodissected, and cultured organoids were removed from Matrigel substrate, then RNA extraction was performed according to the PicoPure RNA Isolation Kit protocol. Reverse transcription and cDNA amplification was performed according to Ovation RNA-Seq Systems V2 protocol (NuGEN). For each sample a minimum total input RNA amount of 500 pg was used. RNA-Seq library preparation was performed according to the NuGEN Ovation Rapid DR Multiplex System 1-8 protocol.
GEO Accession
GSM2527234