GSM1838920: MT1 HAT mutant BRD4 RNA-seq; Homo sapiens; RNA-Seq
For Mnase-seq Lysates were clarified from sonicated chromatin to obtain mononucleosomes, followed by DNA purification. For RNA-seq, total RNA was extracted using Trizol reagent (Invitrogen), mRNA purified using the Dynabeads direct mRNA purification kit (Life technologies, Cat no. 61011), converted into cDNA using standard protocols and used for library construction Libraries were prepared according to Illumina's instructions. Briefly, DNA was end-repaired, an 'A' overhang added, ligated to Illumina's adapters.  After adapter ligation DNA was PCR amplified with Illumina primers and library fragments were band isolated from an agarose gel. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols.