GSM4256505: MEF-TC1; Mus musculus; RNA-Seq
Cell RNA were harvested using Trizol reagent. Poly(A) RNAs were purified by using the Dynabeads mRNA purification kit and Poly(A) RNAs were fragmented at 94C for 5 min before reverse transcribed to cDNA with random primers. cDNA fragments were blunted and ligated to sequencing adaptors. Libraries were sequenced with the High-Seq 2000 Illumina sequencing platform RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM4256505