GSM4386315: Sample22_7d_CD_5; Homo sapiens; Bisulfite-Seq
DNA from whole blood was extracted using a QIAamp DNA Blood Mini Kit (Qiagen: Cat. #51104). For DNA extraction from dried blood spots, one half of a single blood spot  79 was cut into small pieces and incubated with lysis buffer (1218μL) and Proteinase K (33μL) using Gensolve reagents (GenTegra: GVR-113) in an Incubator/vortex shaker set at 1400rpm for 90min, at 56°C (ThermoMixer® C, Eppendorf). The lysate was processed using a QIAamp DNA Blood Mini Kit (Qiagen: Cat. #51104). RRBS libraries were generated from 100ng of high-quality dsDNA using the RRBS Methyl_seq System 1-16 (Ovation: Part #0353) and EpiTect Fast DNA Bisulfite kit (Qiagen: Cat. #59824) following the manufacturer's protocols.
GEO Accession
GSM4386315