Illumina HiSeq 2000 sequencing; Stress responses of Candida albicans
Stress responses of Candida albicans
Candida albicans (strain CAI4-CIp10) was grown in YPD-T (Tris buffered YPD: 2% w/v glucose, 2% w/v mycological peptone, 1% w/v yeast extract, 100 mM Tris.HCl, pH 7.4) to mid-exponential phase Cultures were split and exposed to stresses in fresh medium at an OD600 of 0.2. Biological triplicate cultures were processed After 10 min cells were flash frozen in liquid nitrogen and RNA was extracted using Qiazol (phenol/chloroform  method). An unstressed sample (timepoint 0 min) was used as control. RNA was DNase treated using Ambion Turbo DNAfree kit before being sent to GenePool. Libraries were prepared using standard Illumina RNAseq library preparation kit.
Experimental Factor: growth condition
0.4 mM H2O2 , 30 min