16S-Seq of subgingival plaque from dental implant
The v1-v3 hypervariable regions of 16S ribosomal RNA genes of the bacterial were amplified using PCR. The primers were designed as 5-AGAGTTTGATCCTGGCTCAG-3 forward, and 5-ACTCCTACGGGAGGCAGCAG-3 reverse, and the 10-nt barcodes were tagged to the 5 position. PCR was performed following the instruction of the GS FLX Amplicon DNA library preparation method (Roche, Mannheim, Germany).