Identification of molecular biomarkers indicative of hydrocarbon exposure in Crassostrea virginica
Gill and digestive gland tissues were isolated from multiple C. virginica collected from the MS and AL coast of the Gulf of Mexico in early June during the 2010 Deepwater Horizon oil spill.  Total RNA isolated from multiple individual samples were pooled to generate separate total RNA pools from gill and digestive gland. mRNA was purified from total RNA using the MPG mRNA Purification Kit (PureBiotech, USA) and cDNA was synthesized using the cDNA Synthesis System Kit with random primers (Roche, USA). A cDNA Rapid library was constructed with the GS FLX Titanium Rapid Library Preparation Kit and emulsion-based PCR amplification of the library was performed using a GS FLX Titanium LVemPCR Lib-L Kit.    For Illumina sequencing, total RNA from gill and digestive gland samples were pooled for library preparation and paired-end sequencing performed by the Genomics Service Laboratory at the HudsonAlpha Institute for Biotechnology using the HiSeq 2500 platform.