GSM1356190: PARP-1 ChIP-seq replicate 4; Drosophila melanogaster; ChIP-Seq
MNase digested nuclei and histone-DNA complexes were isolated with specific antibody. Libraries were prepared according to Applied Biosystems protocol for SOLiD4 fragment paired-end sequencing. Briefly, DNA was end-repaired and 5’-phosphorylated by incubation in DNATerminator end-repair kits, as recommended by the manufacturer (Lucigen Corp., Middleton, WI). SOLiD adapters were ligated and the DNA molecules PCR amplified (very low cycle number) and sequenced by using the Applied Biosystems protocol for SOLiD fragment paired-end sequencing. After adapter ligation DNA was PCR amplified with SoLiD primers for less than 12 cycles and library fragments of ~250 bp (insert plus adaptor and PCR primer sequences) were band isolated from an agarose gel. The purified DNA was captured on a SoLid flow cell for cluster generation.
GEO Accession
GSM1356190