GSM2190610: total RNA DIS3L2 mut rep1; Homo sapiens; RNA-Seq
For RNA isolation, cell lines were treated with doxycycline (100 ng/ml) for 3 days before RNA was isolated using the standard TRI Reagent (Sigma-Aldrich) procedure according to the manufacturer’s guidelines. For RNA-Seq we used RNA samples treated with DNase (Ambion) and rRNA-depleted using a Ribo-Zero Kit (Epicentre) according to the manufacturer’s protocol. For samples labeled "total-rRNA" strand-specific RNA libraries were prepared in triplicate using a TruSeq RNA Sample Preparation Kit (Illumina) as per the manufacturer’s protocol with several previously described modifications (Szczepinska et al. Genome Res. 2015); For samples labeled "small RNA" RNA molecules underwent additional TAP (Epicentre) treatment. Next we used RA3, RA5 and RTP, RP1 and RPI_X (Illumina) for library preparation (detailed protocol can be sent on demand). Prepared libraries were size selected through gel purification.
GEO Accession
GSM2190610