GSM3660282: M1 input; Homo sapiens; MeDIP-Seq
The genomic DNA in each group was extracted using a phenol and chloroform method and sonicated with a Bioruptor sonicator (Diagenode) to generate 200–700 bp fragments. The libraries were constructed following the Illumina genomic DNA protocol and adaptor-ligated DNA was immunoprecipitated using 5′-methylcytidine antibodies. The MeDIP products and supernatant DNA were then purified using MinElute columns (Qiagen, Valencia, CA, USA) and eluted in 16 μL of elution buffer (EB; Qiagen). The products were verified using qPCR, with 5 μL of DNA examined per sample using single-end Illumina PCR primers. The methylated DNAs were amplified in situ using a TruSeq Rapid SR Cluster Kit (#GD-402-4001, Illumina), and the quality of the fragments between 300–800 bp was evaluated using an Agilent BioAnalyzer. Sequencing was performed using an Illumina HiSeq 4000 according to the manufacturer's instructions.
GEO Accession
GSM3660282