GSM4385394: crz1Δ_12h_1_Strand_specific; Cryptococcus neoformans var. grubii H99; RNA-Seq
Total RNA was subsequently extracted from the cells collected at different time points. The RNA concentration of each sample was assessed using a Qubit RNA Assay Kit on a Qubit 2.0 Fluorometer (Life Technologies, CA, USA). The RNA integrity was evaluated using the RNA Nano 6000 Assay Kit with a Bioanalyzer 2100 system (Agilent Technologies, CA, USA). The transcriptome libraries were generated using the VAHTS mRNA-seq v2 Library Prep Kit (Vazyme Biotech Co., Ltd, Nanjing, China) following the manufacturer's instructions. Transcriptome library sequencing was performed by Annoroad Gene Technology Co., Ltd (Beijing, China). The samples were clustered using VAHRS RNA adapters set1/set2 and then sequenced on an Illumina platform.
GEO Accession
GSM4385394