GSM2795497: CFBE RPF with RPL12 siRNA treatment, harringtonine chased for 90s; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol reagent (Invitrogen) and the sample was enriched in mRNA using the Ribo-Zero Gold rRNA Removal Kit (Illumina) and spiked with ERCC RNA Spike-In Mix(Thermo Fisher). RNA-size selection and generation of the cDNA libraries was performed as described {Ingolia, 2009}
GEO Accession
GSM2795497