GSM1842185: anti-SDG711; Oryza sativa Japonica Group; ChIP-Seq
Leaves were harvested and fixed in formaldehyde for chromatin cross-linking, About 2g of seedling leaves were crosslinked in 1% formaldehyde under vacuum. Chromatin was extracted and fragmented to 200-750 bp by sonication, and ChIP was performed using the following antibodies: H3K27me3 (a2363, ABclonal) and Anti-SDG711. Seedling leaves were harvested and frozen in liquid nitrogen for DNA extraction using the DNeasy plant mini kit (Qiagen). DNA from ChIP was used to construct sequencing libraries following the protocol provided by Illumina TruSeq® ChIP Sample Prep Set A. Genomic DNA (5g) was used to generate BS-Seq libraries as described in Feng et al., 2011.
GEO Accession
GSM1842185