Deep sequencing of hepatic small RNA libraries from blunt snout bream
Small RNAs (16–30 nt) were isolated from the total RNA samples by size fractionation using 15% denaturing polyacrylamide gel electrophoresis. Proprietary adaptors (Illumina, San Diego, USA) were then ligated to the 5’ and 3’ ends of the small RNAs and reverse transcription was performed according to the Illumina protocol. The generated small cDNA libraries were amplified by PCR using primers complementary to the adaptor sequences.