ChIP-Seq of CEBP-Beta in Rattus norvegicus:  regenerating liver
Adult (275-350g) Sprague-Dawley rats were fed using the Lieber-DeCarli pair-feeding model in which ethanol diet comprises 36% total calories from ethanol, 11% from carbohydrate, 18% from protein and 35% from fat and in isocaloric carbohydrate control diet ethanol is replaced with 36% amount carbohydrate calories. After 5-7 weeks of ethanol feeding, they were anesthetized and subjected to 70% partial hepatecomy by surgical removal of medial and left lateral lobes as per standard procedure. The medial and left lateral lobes were flash frozen using liquid nitrogen-cooled aluminum clamps to serve as within-animal, 0 hour controls. At 6 hours post-PHx, rats (three biological replicates per diet) were again anesthetized and the remnant liver tissue was excised and flash-frozen as before. Following excision of the remaining liver mass, rats were sacrificed by cervical dislocation. Liver tissue from 0h and 6h post-PHx was subjected to chromatin immunoprecipitation. Immunoprecipitated samples (ChIP) from 0h and 6h post-PHx were used to identifiy genome-wide C/EBP-a and C/EBP-b binding sites using ABI SOLiD sequencing (ChIP-seq).