GSM2871660: ATAC Control replicate 3; Mus musculus; ATAC-seq
50,000 cells were lysed in 10mM TrisHcl ph7.4, 10mM NaCl, 3mM MgCl2, 0.1% IGEPAL. Nuclei were incubated with Tn5 transposase and 2% Digitonin. Then DNA was purified with Qiagen MiniElute kit. 10 μl of DNA was combined with 2.5 μl of indexing primers (Nextera Customized PCR Primer 1 and barcode Nextera PCR Primer 2), 25 μl of NEBNextHigh-Fidelity 2x PCR Master Mix (New England Biolabs). DNA was then amplified for 8 cycles to enrich the tagmented DNA fragments. A PCR clean-up was then performed using AMPure XP beads (Beckman Coulter), and the small fragments were then resuspended in 32.5 μl of resuspension buffer (provided in the Nextera kit). DNA was quantified using a Qubit fluorometer (Life Technologies), and library sizes were then determined using TapeStation (Agilent Technologies)
GEO Accession
GSM2871660