ssRNA-Seq of XK (no treatment)
A heat sensitive variety GHA and a tolerant variety XK of non-heading Chinese cabbage (B. rapa ssp. chinensis) were used in this study. For heat treatments, 3-week-old leaves were grown at 37C (high temperature) for 1 and 12 h. For control samples (CK), the leaf discs were incubated at 25C. Total RNA was isolated from the leaves at different stages using Trizol Reagent.For ssRNA-seq library construction, 5 g of total RNA from each sample was used for rRNA removal using the Ribo-Zero rRNA Removal Kit. Sequencing libraries were generated using the NEBNext Ultra Directional RNA Library Prep Kit according to the manufacturers instructions. Then, comprehensive landscape of dynamic lncRNA expression networks under heat stress were analyzed.