GSM1654419: Delto-Smu-4-1; Populus deltoides; RNA-Seq
RNA was also isolated from 100 mg of liquid nitrogen chilled homogenized leaf tissue using the RNeasy Plant Mini Kit (Qiagen) with in column RNase-Free DNase Set (Qiagen) . RNA was extracted from each tree sampled and assayed for quality with a 2100 Bioanalyzer and Nanodrop 1000 spectophotometer. All RNA samples had a ratio of the absorbance at 260 and 280nm (A260/280) above 2.0 and were confirmed to be free of genomic DNA. Libraries for each RNA sample were prepared by Brian Boyle at ‘L'Institut de biologie intégrative et des systèmes’ (IBIS) at Université Laval, Quebec City, Quebec, Canada. Each cDNA library was barcoded and six samples were pooled together. TruSeq RNA Sample Preparation Kit v2 was used to prepare DNA for Illumina sequencing
GEO Accession
GSM1654419