GSM3350623: NDMM3_C69; Homo sapiens; RNA-Seq
Bone marrow aspirates were collected from 15 patients after informed consent and subjected to ACK lysis and mononuclear cell isolation. Plasma cells were separated by positive selection using CD138 coated magnetic beads (MACS; Miltenyi Biotec, CA) in a RoboSep system (STEMCELL Technology, Canada). CD138 positive cells were examined using Vi-CELL XR (Beckman Coulter, CA) to determine cell number, viability and average size. A microfluidic mRNA-Seq chip (Fluidigm, CA) was used for capturing cells from each sample at a concentration of 500 cells/μl and run in the Fluidigm C1 system to  generate double stranded cDNA using SMARTer Ultra Low RNA kit for Illumina (Takara, CA).  All samples were assessed for cell capture in a C1 chip by direct observation under a microscope and for cDNA quality using Fragment Analyzer (HS Large Fragment kit, Advanced Analytical Technologies, IA).
GEO Accession
GSM3350623