Human plasma primary virome sequencing: Sample M1179288
Total nucleic acids were extracted using the MagMax Viral RNA Isolation Kit. SuperScript II RT and random hexamers were used to reverse transcribe the Viral RNA. Molecules were amplified and tagged with a BC12-V8A2 construct31 using AccuPrime Taq polymerase and cleaned with ChargeSwitch kit. Viral amplicons were then normalized, pooled, and made into an Illumina library. The library was then loaded and sequenced on the Illumina HiSeq2000.