Latyhrus sativus BGE015746 inoculated with U. pisi
Two-week-old L. sativus seedlings were inoculated by dusting all the plants at the same time with 2 mg of spores per plant, diluted in pure talk (1:10), with the help of a small manual dusting device in a complete random experiment. Inoculated and control plants were incubated for 24 h at 20 ºC, in complete darkness, and 100% relative humidity, then transferred to a growth chamber and kept at 20 ± 2 ºC under 14h light (150 µmol m-2 s-1) and 10h dark. RNA was extracted from inoculated and non-inoculated fresh leaves collected 37 hours after inoculation.