RNA-Seq of root of Camellia oleifera
Total RNA was extracted using the Trizol reagent (Invitrogen, CA, USA) according to the manufacturers instructions. The quantity and purity of the total RNA were analyzed using a Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0 as the criterion. One RNA sample was collected for each of the 12 tissue samples. RNA-Seq of every sample was conducted separately to yield a gene expression profile. Approximately 10 g of total RNA was used to isolate poly-A mRNA with poly-T oligo-attached magnetic beads (Invitrogen, USA) for purification. Subsequently, the cleaved RNA fragments were reverse-transcribed to create the final cDNA library following the protocol of the mRNA-Seq sample preparation kit (Illumina, San Diego, USA); the average insert size for the paired-end libraries was 300 bp (50 bp). Finally, paired-end sequencing was performed on an Illumina HiSeq 4000 at Lc-bio (China) in accordance with the vendors recommended protocol.