NextSeq 500 paired end sequencing
Targeted exome sequencing for SNP and CNA analysis
Total genomic DNA from purified cells and isolated tissues was purified with DNeasy Blood & Tissue Kit (Qiagen) and quantified by Qubit and NanoDrop measurement. Volume of samples with low concentrations (<25ng/Œºl) was reduced by SpeedVac. 50ng of genomic DNA was used as input for SureSelect QXT library (Agilent) preparation using the standard protocol. Successful pre-hybridization library preparation was followed by Qubit and Labchip GX touch measurement for quantity and insert size. 200-1.500ng of library was used for hybridization with SureSelect Mouse All Exon Kit (Agilent) to enrich for Exome-containing library elements.