GSM1481098: mRNAs from Burkholderia gladioli BSR3 wild type after 24h LB liquid culture; Burkholderia gladioli; RNA-Seq
All bacteria strains were grown in 2 ml of LB broth at 28°C with continuous shaking until reach mid-expotential phase (OD600 = 0.5), and subcultured for 10h or 24h in identical condition. Total RNA was extracted from 4 ml of each bacterial cultures using RNeasy Midi Kit (Qiagen, Valencia, CA) and residual genomic DNA contamination was subsequently removed using an RNase-Free DNase set (Qiagen, Valencia, CA) according to the manufacturer’s protocols. After DNase I treatment, a MICROBExpressTM bacterial mRNA enrichment kit (Ambion, Carlsbad, CA) was used to remove bacterial rRNA from the total RNA sample. RNA libraries were prepared for sequencing using standard Illumina protocols.
GEO Accession
GSM1481098