RNA-Seq of Nymphaea thermarum: root
Libraries for RNA-Seq analysis were prepared by the Whitehead Genome Technology Core, from total RNA (200-500ng) with the Apollo 324 system from WaferGen Biosystems using the WaferGen Prep-X Directional RNA-Seq kit according to manufacturers protocols to produce strand-specific cDNA libraries. Adapter-ligated cDNA fragments were enriched and amplified with 15 cycles of PCR using the HiFi NGS Library Amplification kit from KAPA Biosystems. Libraries were quantified by qPCR using the KAPA Biosystems Illumina Library Quantification kit according to kit protocols. Libraries with distinct TruSeq indexes were multiplexed by mixing at equimolar and sequenced on the Illumina HiSeq 2500 for 40 bases in single-read, high-output mode.