GSM4101212: 750s-to-380_r1_RNA.fastq; Trichodesmium erythraeum IMS101; RNA-Seq
Cultures were gently filtered onto 5 µm polycarbonate filters (Whatman) during the middle of the photoperiod, immediately flash frozen, and stored in liquid nitrogen until RNA extraction. RNA was extracted with the Ambion MirVana microRNA isolation kit (Thermo Fisher Scientific) in an RNase-free environment in accordance with the manufacturer's instructions, followed by two incubations with Ambion's Turbo DNA-free kit to degrade trace amounts of DNA. Then the Ribo-Zero rRNA Removal Kit (Illumina) was used to remove rRNA. RNA was then submitted to the UC San Diego IGM Genomics Center for library construction and sequencing using the TruSeq Stranded mRNA Library Prep kit (Illumina). Multiplexed libraries were sequenced by using Illumina Hi-Seq4000, yielding single-end 50-bp read libraries.
GEO Accession
GSM4101212