GSM3783655: shRNA CDAN1 (codanin-1) rep2; Homo sapiens; RNA-Seq
Total RNA was isolated on day 12 of the erythroid culture using with TRIzol (Invitrogen) or the RNeasy kit (QIAGEN). DNA contamination was removed with DNase I treatment (Roche). RNA concentration and purity (A260/A280 above 1.8) were assessed using a Nanodrop ND-8000 spectrophotometer (NanoDrop Technologies). RNA libraries were prepared for sequencing using the high-throughput Illumina platform at the Yale Center for Genome Analysis.
GEO Accession
GSM3783655