Drosophila mauritiana mau12w large-insert genomic DNA library
mau12 is a white-eyed inbred laboratory stock (14021 0241.60). Adult females reared on standard cornmeal food. Genomic DNA from whole flies extracted using the DNeasy Blood and Tissue kit (Qiagen, Valencia, CA). DNA concentration was determined with the Qubit Flourometer (Life Technologies, Grand Island, NY) and quality was assessed using the Agilent Bioanalyzer (Agilent, Santa Clara, CA). Mate pair libraries were generated using the Nextera Mate Pair protocol (Illumina, San Diego, CA). Briefly, 4 μg of genomic DNA was fragmented per manufacturer’s protocol followed by strand displacement, agarose gel size selection of 5 kb target size, and circularization. The 5 kb fragments were sheared using a Covaris S2 (Covaris Inc., Woburn, MA) to 300 to 1000-bp size fragments for end repair, A-tailing, and indexed adaptor ligation. The amplified libraries were purified by AMPure (Beckman Coulter, Brea, CA) purification and hybridized to an Illumina paired-end flow cell for cluster amplification using the cBot (Illumina, San Diego, CA) at a concentration of 8 picomoles per lane.