GSM4100879: Xrcc4-/- p53-/- Released; Mus musculus; OTHER
Genomic DNA was extracted with proteinase K digestion overnight and phenol/chloroforme extraction. Briefly, 3 ug gDNA was sonicated to 150 to 200 bps size. Sheared DNA was end repaired, dA tailed, ligated with provided paired-end adaptor and amplified. The prepared DNA was hybridized to customized probes and captured by Dynabeads MyOne Streptavidin T1 (Life Technologies). The pull-downed DNA was amplified with provided indexing primers of Illumina MiSeq for 250 bps paired-end sequencing.
GEO Accession
GSM4100879