Ion Torrent Proton sequencing
Single-end sequencing of DNA sample isolated from microbiological community inhabiting weathered Kupferschiefer black shale, fragmented by sonication, ligated with sequencing adapters and amplified.
Isolated DNA was used to prepare barcoded library with Ion Xpress™ Plus Fragment Library Kit (Thermo Fisher) according to manufacturer's manual. Library was subjected to clonal amplification on Ion One Touch 2 system (Thermo Fisher) using Ion PI™ Template OT2 200 Kit v2 (Thermo Fisher) and sequenced on Ion Proton sequencer using Ion PI™ Sequencing 200 Kit v2 (Thermo Fisher) according to manufacturer's manual.