GSM4928732: 5XMTHF_rep1; Rattus norvegicus; RNA-Seq
Whole brains were removed immediately upon termination and the Arcuate nucleus of the hypothalamus was later macrodisected on dry ice using approximate stereotaxic coordinates. RNA was harvested using Trizol reagent. Illumina HiSeq 2500 was performed with polA mRNA selection for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM4928732