Responses of eukaryotic stream microbes to multiple anthropogenic stresors in a mesocosm study
Amplification of V9 of the 18s SSU+ ITS1 using Euk1391F (Lane 1991; Lange et al. 2015) and ITS2 (White et al. 1990). Two PCR replicates per sample allowed for the application of ampliconDuo filtering.