GSM3405610: Persister cells - cells treated with ciprofloxacin 2; Pseudomonas aeruginosa; RNA-Seq
RNA was isolated from untreated and persister cells using the RNeasy Kit (QIAgen) according to manufacturer's instructions and treated twice with TURBO DNase (Ambion, Grand Island, NY) to remove any contaminating DNA (5, 6).  Ribosomal RNA was depleted using the Ribo-Zero Magnetic kit (Epicentre, Madison, WI) Directional library was generated using the NEXTflex directional RNA-seq kit (Bioo Scientific, Austin, TX)
GEO Accession
GSM3405610