RNA-seq of neonatal lung endothelial cells; 24 hr LPS treatment
Total RNA was extracted from mouse lung EC using the mirVana miRNA isolation kit (Thermo-Fisher). RNA sequencing was performed on a HiSeq 1500 system (Illumina, San Diego, CA). Mapping of RNA-Seq reads and transcript assembly and abundance estimation were conducted using the Tuxedo Suite software package (Broad Institute, Cambridge, MA).