Illumina HiSeq 2000 sequencing; H3K4me3 and H3K27me3 chromatin states in the Arabidopsis shoot phloem companion cells from INTACT reporter lines
H3K4me3 and H3K27me3 chromatin states in the Arabidopsis shoot phloem companion cells from INTACT reporter lines
Plants of the shoot-phloem-companion-cell-tagged INTACT reporter line (Transgenic SUC2::redNTF and UBQ10::BirA in Col-0 background) were cultivated in growth chambers, in short-day (SD) conditions (8 h light/16 h dark) for 21 days and then shifted to long-day (LD) conditions (16 h light/8 h dark) to induce flowering.  The growth-chamber condition was at 23°C with 65% relative humidity, and with the light at a fluence rate of 125 to 175 μmol m−2 s−1. ChIP DNA was prepared from INTACT purified nuclei from the meristem, as described in Deal, R. B. & Henikoff, S.  (2010) Nat. Protoc.  6, 56-68; and You et al. (2017): DOI: 10.1038/ncomms15120. Libraries were prepared from approximately 50 pg of ChIP DNA using the ThruPlex-FD Prep Kit (Rubicon Genomics)
Experimental Factor: immunoprecipitate
H3
Experimental Factor: sampling time point
2