GSM1482906: siNELF replicat1; Homo sapiens; RNA-Seq
Total RNAs whole-cell RNAs were depleted for ribosomal RNAs using the Ribo-ZeroTM Magnetic Gold Kit following manufacturer’s instructions.For total RNA samples (siInt11_1/2/3, SiNELF-1/2-3; Siscrambled-1/2/3), 100 ng of ribosomal RNA depleted whole cell RNA was used for library construction following Illumina’s instructions. cDNA libraries of poly-adenylated RNAs ('Int11-1/2-3' NELF-1/2/3; scr/Scrambled-1/2/3) were generated using 1 µg of whole-cell RNAs following Illumina’s instructions. Librairies were prepared using Illumina Tru-Seq stranded mRNA sample prep kit TruSeq Sample Prep Kit (multiplexed libraries) according to manufacturer’s instructions.(Illumina).
GEO Accession
GSM1482906