16SrRNA sequencing of fecal sample
DNA from samples was extracted using CTAB/SDS methodAll PCR reactions were carried out with Phusion High-Fidelity PCR MasterMix (New England Biolabs)Sequencing libraries were generated usingTruSeq DNA PCR-Free Sample Preparation Kit (Illumina, USA) following manufacturer's recommendations and index codes were added. The library quality was assessed on the Qubit@ 2.0 Fluorometer (Thermo Scientific) and Agilent Bioanalyzer 2100 system. At last, thelibrary was sequenced on an IlluminaHiSeq2500 platform and 250 bp paired-end reads were generated