CK24h_3_1
mRNA with polyA tail was isolated using oligo(dT) attached with magnetic beads. mRNA was fragmented by fragmentation buffer, and the double-strand cDNAs were synthesized by random hexamers and RNase H and DNA Polymerase I. These cDNA fragments then have the addition of a single A base and subsequent ligation of the adapter. The products are then purified and enriched with PCR amplification. The double stranded PCR products were heat denatured and circularized by the splint oligo sequence. The single strand DNA circle (ssDNA circle), which was the final library.