in-situ BPA anaerobic degradation experiment-ac0
The study was to investigate the shift in bacterial community structure associated with BPA degradation in river sediment under nitrate- or sulfate-reducing condition.  Anaerobic sediment microcosms consisted of mineral salt medium, BPA, sediment and supplementary electron acceptor (NaNO3 or Na2SO4) in serum bottles (150 mL). Different sets of treatments in triplicate were performed: (A) sediment +NaNO3; (C) sediment+BPA+NaNO3;(D) sediment + Na2SO4; (F) sediment+BPA+Na2SO4. Microcosms were incubated at 28oC on a horizontal shaker (140 rpm) for 120 days. Bacterial genome DNA was extracted at 0day, 75day and 120day. The bacterial community structure was characterized using Illumina MiSeq sequencing.