GSM2796553: 36Y Female HSCe 61; Homo sapiens; RNA-Seq
Single cell CD34+CD38- HSCe were prepared using the C1™ Single-Cell Auto Prep System (Fluidigm, San Fransisco, CA), according to the manufacturer’s instructions. Flow-sorted cells were counted and resuspended at a concentration of 350 cells per mL PBS then loaded onto a primed C1 Single-Cell Auto Prep Integrated Fluidic Chip for mRNA-Seq (5-10 mm). cDNA was made with Clontech SMARTer® Kit “the mRNA Seq: RT + Amp (1771x), Libraries were constructed using Nextera XT DNA Library Preparation Kit Single cells were separated using Fluidigm C1 system
GEO Accession
GSM2796553