GSM4101472: Liver_ChIPSeq_H3K27Ac_LapTTA_4W; Mus musculus; ChIP-Seq
For ChIP experiments, whole cell extracts were sonicated to solubilize the chromatin. The chromatin extracts containing DNA fragments with an average size of 250 bp were immunoprecipitated using different antibodies. For TEAD, HNF4a, BRD4, Histone Marks, RNAPol2 ChIP dissected liver/tumors were fixed with 1% formaldehyde. For YAP ChIP, fixation was performed by a double step approach with 0.5 M DSG (Di-(N-succinimidyl)-glutarate)  and 1% formaldehyde (FA).                           For ATAC seq experiments, 100,000 cells obtained from liver perfused mice were lysed to obtain nuclei. Transposition reaction and DNA purification were then performed. For ChIP seq, libraries were prepared for Illumina sequencing using a standard protocol consisting in blunting, addition of dA overhangs, ligation of Illumina adapters, selection on gel and PCR with index primers. For ATAC seq, libraries were prepared for Illumina sequencing with index primers.
GEO Accession
GSM4101472