GSM1197604: EBNALP rep2; Homo sapiens; ChIP-Seq
EBNALP-DNA complexes were captured with the addition of protein A-Agarose/Salmon sperm DNA and then washed 1x for 15’ at 4°C with rotation, with  Low Salt Wash Buffer (20mM Tris-HCl pH 8.1, 2 mM EDTA, 150 mM NaCl, 1% Triton X-100, 1% SDS), 1x with High Salt Wash Buffer (20mM Tris-HCl pH 8.1, 2 mM EDTA, 500 mM NaCl, 1% Triton X-100, 1% SDS), 1x with LiCl Wash Buffer (10 mM Tris-HCl pH 8.1, 1 mM EDTA, 0.25 M LiCl, 1% NP40, 1% Deoxycholate acid), and 2x TE buffer. EBNALP-DNA complexes were eluted from the protein A-beads by rotating at room temperature 2x for 15’ in Elution Buffer (100 mM NaHCO3, 1% SDS). DNA-protein cross-link was reversed by the addition of NaCl and incubation at 65°C for 4 h. DNA was purified using a PCR purification column (Qiagen®). ChIP-seq DNA libraries were prepared and sequenced, using Illumina HiSeq 2000. Libraries were prepared according to Illumina's instructions
GEO Accession
GSM1197604