GSM4386987: US-40-3; Homo sapiens; RNA-Seq
Total RNA was extracted from cells harvested at 48 h, 7 days, and 28 days of exposure to GO-US, GO-S, and GO-L using the RNeasy Mini Columns (Qiagen) in accordance with manufacturer's instructions (including the purification step with DNase I). Total RNA concentration was determined spectrophotometrically using NanoDrop (NanoDrop Technologies). Quality control was conducted using the Bioanalyzer 2100 (Agilent Technologies) and all samples had RNA integrity numbers (RIN) above 8. Triplicates of each sample at different time-points and exposures were submitted for sequencing. Illumina TruSeq Stranded mRNA Library Prep Kit (Illumina, Inc., San Diego, CA, USA) was used to prepare mRNA sequencing libraries. Briefly, 1000 ng of total RNA was used for mRNA isolation using poly dT-coated beads
GEO Accession
GSM4386987