GSM3579675: Exo-Hi-C (LRC-Hi-C-1); Mus musculus; Hi-C
Confluence of the cell should be around (80-90%).  Add HCHO to 1% the plate (280ul 37% HCHO to 10 ml medium) and incubate at 37°C for 10 min; Stop the cross linking by adding 0.89 ml 2.5M glycine to medium (final concentration 0.2M) at RT for 5 mins, aspirate the medium. Wash the cells twice with 10 ml of 1xPBS.Aspirate the buffer (keep 0.5 ml in the plate). Harvest cells by scraping. Wash the plate once with 0.5% BSA/PBS. Combine the cells from the plates. Spin down the cells at 500-1000g, 5 min, 4℃. Following the proximity-ligation and linear noise elimination(exonuclease combination treatment), the chromosome conformation capture library was used for sonication and immunomagnetic interaction capturing of biotin labeled joint DNA. Hi-C library was submitting for next-generation sequencing.
GEO Accession
GSM3579675