GSM2527784: CEF_rep2; Gallus gallus; Hi-C
Embryonic fibroblasts were extracted from 14.5-day-old embryos as described elsewhere 77. Each embryo was processed individually. Briefly, embryo was decapitated, chopped into pieces of approximately 1 mm3 and incubated with 0.025% trypsin solution for 20 min. Trypsin was quenched using a medium containing fetal bovine serum (FBS), cells were centrifuged and plated on Petri dishes. Isolated embryonic fibroblasts were cultured in DMEM medium supplemented with 10% FBS at 40°C, 5% CO2. For each sample (red blood cells and fibroblasts) two independent fixations (biological replicas) were performed. All Hi-C libraries were prepared using in situ protocol, as described in Rao S.S. at all Cell (PMID: 25497547). DNA libraries were sequenced from both ends on Illumina HiSeq 2500 platform in 3 runs total. The sequencing was conducted in 2x101 and 2x106 mode with TruSeq Rapid SBS chemistry, and in 2x126 mode using HiSeq® SBS Kit v4
GEO Accession
GSM2527784