GSM1482114: F1_B6xCast_R1; Mus musculus; RNA-Seq
Retinas were dissected in cold sterile HBSS with calcium and magnesium (Gibco) and stored at -80 ⁰C until use. Total RNA was extracted using TRIzol (Invitrogen) and purified using the RNeasy Mini Kit (Qiagen) with on-column DNaseI digestion (Qiagen). Polyadenylated mRNA was captured from total RNA using Dynabeads (Invitrogen). The mRNA was fragmented and reverse-transcribed to double-stranded cDNA using random hexamers. The cDNA was blunt-ended and 3’-adenylated before ligation to sequencing adapters. Ligated fragments were amplified for 12 cycles with primers to incorporate unique sample barcodes. Libraries were subjected to paired-end 2x101 bp sequencing on the Illumina HiSeq 2000. One lane of sequencing was conducted for all F0 and F1 samples, and a second lane of sequencing was conducted for the F1 samples only.
GEO Accession
GSM1482114