GSM3721978: 12Z_ChIP_ARID1A_IP2; Homo sapiens; ChIP-Seq
Cells were scraped in PBS, and 1x10^7 cells were used per IP. Crosslinked chromatin was digested with MNase using the SimpleChIP Enzymatic Chromatin IP Kit (Cell Signaling) followed by 30 seconds of sonication. ChIP was performed with the SimpleChIP Enzymatic Chromatin IP Kit with 1:100 anti-ARID1A (12354, Cell Signaling). DNA was purified using the ChIP DNA Clean & Concentrator Kit (Zymo). Libraries were prepared from 10 ng of input material and all IP material using the KAPA Hyper Prep Kit (v5.16) (Kapa Biosystems). Libraries were pooled and sequenced to 75 bp single-end reads on an Illumina NextSeq 500.
GEO Accession
GSM3721978