contaminated soil with low Cd rep3
The metagenomic DNA was extracted from 0.25 g of each sample of contaminated and uncontaminated soil. We worked with the ZR soil microbe DNA MiniPrepTM extraction kit (Epigenetics CompanyTM) following the manufacturer's instructions.  The DNA samples were sent to the Molecular Research laboratory (Mr.DNA) of Shallowater, Texas, USA, for sequencing in Illumina Miseq 2x300 bp, 20K, using the 16S rRNA gene V1-V3 variable regions PCR primers 515/806, which are widely used for taxonomic classifications of bacterial communities