GSM2527048: miRNA_ko_p5; Mus musculus; RNA-Seq
RNA integrity was evaluated by 2100 Bioanalyzer (Agilent Technologies) with RIN no less than 7. RNA-seq was performed by NIH Intramural Sequencing Center (Bethesda, MD). One paired end index library from each  polyA(+)-selected RNA samples was prepared. Six samples were pooled and loaded on 2 lane(s) of Illumina HiSeq 2000 Sequencer, and run as paired end index 100 base reads with a total of 40 million base read pairs per sample. Differential mRNA expression between miR183C knockout mice and agematched C57bl6/J control mice was quantified by RNA-seq data analysis using the Tuxedo package (i.e. the Tophat-Cufflinks pipeline) on the NIH Biowulf high performance computing platform.
GEO Accession
GSM2527048