GSM3721311: ASC Rep 1; Mus musculus; ATAC-seq
Medium was removed and cells were lysed using ATAC-seq lysis buffer ( (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630). Library was constructed based on protocols from Buenrostro et al., (2015). Briefly, lysates were tagmented with Nextera DNA Library Preparation Kit (Illumina) and amplified using NEBNext High-Fidelity 2 x PCR Master Mix (NEB). Library was then sequenced using Illlumina NextSeq 500 (2 x 75).
GEO Accession
GSM3721311