GSM4388189: 53480; Mus musculus; ChIP-Seq
Samples were dissected, shock frozen without delay, and stored in liquid nitrogen until further use. Chromatin was prepared from up to 50 mg tissue using SDS Shearing Buffer and the truChlP Tissue Chromatin Shearing Kit (Covaris, Brighton, UK). ChIPs were run on the IP-Star compact system using the Auto iDeal ChIP-seq kit for histones (Diagenode, Seraing, Belgium). Library preparations were performed according to the TrueSeq LT PCR free or TruSeq Nano DNA Kit instructions (Illumina, San Diego, USA). Sequencing was performed on the Illumina HiSeq 4000 with 76 bp paired-end sequencings.
GEO Accession
GSM4388189