Sequencing of bacterial 16S rRNA from soil samples collected near and distant from subsurface drip irrigation emitter, one system installed in 2012 and the other in 2016.
Total DNA was extracted from 0.25g of soil from each experimental microcosm (n=60) using a PowerSoil extraction kit. DNA was amplified for the Bacterial 16S rRNA subunit (regions V1-V3) using primers 27F (Lane 1991) and 519R (Lane et al 1985). Sequencing libraries were constructed following the BASE protocol (Bisset et al 2016).