rpoC1 amplicons of Prochlorococcus and Synechococcus: coastal North Pacific surface water sample
The rpoC1 gene was PCR amplified using Prochlorococcus- and Synechococcus-specific primers SAC1039R and 5M_newF. A total 4 ng DNA was added to 20 _L PCR reactions and amplified. Platform-specific adaptors and barcodes were then added to the reaction and annealed to the amplicons via a second round of PCR.