GSM1192571: SMORE-seq_WT_TAP+_rep3; Saccharomyces cerevisiae; RNA-Seq
Hot phenol total RNA extraction Poly(A)+ RNA was purified and mixed with 5 units (1 μl) Tobacco Acid Pyrophosphatase (TAP). A parallel reaction without TAP enzyme was also performed. The RNA was combined with 5’ SR Adaptor (NEBNext Small RNA Library Prep Set for Illumina). This RNA was fragmented for 4 minutes at 94C using NEB fragmentation reagent. The RNA was then ligated to a 3’ sequencing adapter followed by reverse transcription and 10 cycles of PCR. PCR products of ~250 bp were selected and subjected to another 8 cycles of PCR
GEO Accession
GSM1192571