Loki GC14 75 non-amplified DNA
DNA was extracted from 7,5 gram sediment using the FastDNA spin kit for soil in conjunction with the FastPrep-24 instrument (MP Biomedicals) following manufacturer’s protocol, except for the addition of polyadenosine as described by Hugenholtz et al. The individual extractions were then pooled and up-concentrated to a final volume of 50 µl using Amicon Ultra-0.5 filters (50.000 NMWL) following the manufacturer’s protocol (Merck Millipore). Nextera libraries (Illumina) were prepared according to the manufacturer’s instructions, using 20 ng of the amplified DNA as input. Due to low yield, a total of 8 amplification cycles were used in the PCR step during which the Illumina barcodes and adapters (NextEra Index kit) were fused, rather than the default 5 cycles.