GSM3351367: IPF_invasive_9; Homo sapiens; RNA-Seq
Totla RNA of each sample was isolated using the Absolutely RNA Microprep Kit (Aligent Technologies, Santa Clara, CA, USA) following the manufacture's recommendations. Libraries for RNA-Seq were prepared with KAPA Stranded mRNA-Seq Kit. The workflow consists of mRNA enrichment, cDNA generation, and end repair to generate blunt ends, A-tailing, adaptor ligation and PCR amplification. Different adaptors were used for multiplexing samples in one lane. Sequencing was performed on Illumina HiSeq 3000 for 1x50 run. Data quality check was done on Illumina SAV. Demultiplexing was performed with Illumina Bcl2fastq2 v 2.17 program.
GEO Accession
GSM3351367