GSM4700192: WT_OPC_ATAC_B; Mus musculus; ATAC-seq
Omni ATAC-Seq was performed on 50,000 OPCs from two independent biological replicates following the protocol outlined in (Corces et al., 2017). In brief, nuclei were extracted from cells and treated with transposition mixture containing Nextera Tn5 Transposase for (Illumina, FC-121-1030). Transposed fragments were then purified using Qiagen MinElute columns (Qiagen, 28004), PCR amplified, and libraries were purified with Agencourt AMPure XP magnetic beads (Beckman Coulter) with a sample to bead ratio of 1:1.2. Libraries were amplified by low cycle number PCR utilizing illumina compatible indexed primers.
GEO Accession
GSM4700192