MiSeq Sequencing Data for NIST RM 8376 Pseudomonas aeruginosa - Vial 1 Rep 1
Independent duplicate libraries were constructed from 8 replicate Pseudomonas aeruginosa microbial genomic DNA reference material (RM8377) vials for a total of 16 libraries the libraries were pooled and sequenced in a single run. Libraries were prepared using the Nextera DNA Sample Prep Kit (FC-121-1030). The 8 vials were barcoded using the Nextera Index Kit (FC-121-1011, lot: 9988423), vial numbers correlate with Ion Torrent data vial numbers. Libraries were sequenced using the the MiSeq 600 cycle Reagent kit v3 (MS-102-3003, box 1 lot:10064179; box 2 lot:10065188). Fastq generated using the MiSeq Reporter version 2.5.1.3 with the GenerateFASTQWorker workflow Version 0.0.0. Vial 1 Rep 1 barcoded: i5 index- N517, i7 index - N703.