GSM3270933: CBX4-/- hMSC induced with lentiviruses expressing Flag-Luc, incubated with Flag Ab during ChIP [Flag-ChIP-Flag-Luc-2]; Homo sapiens; ChIP-Seq
Total RNA was extracted with the RNeasy Mini Kit (Qiagen). To obtain the genomic DNA fragments of interest for ChIP-seq, the cells were crosslinked and lysed, and then the chromatin was sheared into fragments using sonication. Then samples were incubated with anti-H3K4me3 antibody (Abcam, ab8050, lot#GR3190162-1) or anti-FLAG antibody overnight. The DNA was de-crosslinked and extracted on the next day. 1.5 μg of total RNA was used to construct RNA sequencing libraries by NEB Next Ultra RNA Library Prep Kit (NEB) for Illumina following the manufacturer's recommended protocol. The ChIP-sequencing library and copy number variation analysis sequencing library were constructed by NEB Next Ultra DNA Library Prep Kit (NEB) for Illumina according to the manufacturer's instructions.
GEO Accession
GSM3270933