GSM3271585: Week_4_continuous_rep_2; Homo sapiens; RNA-Seq
Cells were rinsed with PBS, trypsinized, and centrifuged for 5 min at 300xg, then washed with 1 mL PBS and centrifuged again. After aspirating away PBS, cells were immediately frozen in liquid nitrogen and stored at -80ºC. At the end of the timecourse, all samples were thawed on ice and total RNA was purified using the Quick-RNA microprep kit (Zymo Research) according to the manufacturer's protocol. 100 ng RNA was used to prepare mRNA libraries using the Universal Plus mRNA-seq kit (NuGEN).
GEO Accession
GSM3271585