GSM3721990: EEflG2_ATAC; Mus musculus; ATAC-seq
Endometrial epithelial cells were magnetically sorted using EpCAM-PE and anti-PE microbeads. Nuclei were isolated in cold lysis buffer (10mM Tris-HCL [pH 7.4], 10 mM NaCl, 3mM MgCl2, 0.1% NP-40) by centrifugation at 500Xg for 10 min. ATAC-seq libraries were prepared using the Nextera DNA Library Prep Kit, puified using the Qiagen MinElute Reaction Cleanup Kit, amplified by PCR and purified using Kapa Pure Beads.
GEO Accession
GSM3721990