GSM1655589: TOV21G Ctrl RNAi For Bub3 rep1; Homo sapiens; RNA-Seq
Arcturus picopure RNA isolation kit (Life Technologies, KIT0204) was used to prepare total RNA from cells. Ribo-Zero™ Gold Kits (Human/Mouse/Rat) (Epicentre, MRZG126) was used to remove both cytoplasmic (nuclear-encoded) rRNA and mitochondrial rRNA from total RNA preparations, and libraries were built using the Illumina TruSeq RNA sample prep kit V2 (Illumina, RS-122-2001). Libraries were sequenced using an Illumina HiSeq 2000. Single end 100 bp reads were obtained.
GEO Accession
GSM1655589