GSM4101238: Tricho_WGBS_750-1; Trichodesmium erythraeum IMS101; Bisulfite-Seq
Three randomly chosen biological replicates from each CO2 treatment were gently filtered onto 5 µm polycarbonate filters (Whatman) during the middle of the photoperiod, immediately flash frozen, and stored in liquid nitrogen until extraction DNA extraction. DNA was extracted from frozen filters with the FastDNA Spin Kit for Soil (MP Biomedicals, Santa Ana, CA, USA) following the manufacturer's protocol. Extracted DNA was then sent to the USC Epigenome Center for library construction and sequencing. Briefly, ~100 ng of DNA was bisulfite treated with the Zymo Gold kit (Zymo Research) and libraries were constructed using the Ovation Ultra-Low Methyl-Seq library kit (NuGEN) followed by sequencing on the NextSeq (Illumina).
GEO Accession
GSM4101238