Blast Crisis Chronic Myeloid Leukemia Patient #2
Library Construction:  The exome library was generated from 1ug gDNA extracted with Invitrogen PureLinkTM Genomic DNA (gDNA) Kit (Invitrogen, Life technology, Grand Island, NY, USA). gDNA was fragmented to a size of 500-100bp using a Bandelin Sonopuls sonicator and then processed according to standard Illumina TruSeqTM DNA Sample Preparation Kit (FC-121-1001) protocol. The library was generated with the Illumina TruSeqTM Exome Enrichment Kit (FC-121-1008) and subsequently sequenced on an Illumina Genome Analyzer IIx with 76 bp paired-end reads using Illumina TruSeqTM SBS kit v5 (FC-104-5001). Experimental design: for each patient the genomic DNA was collected from the Blast Crisis (BC) Chronic Myeloid Leukemia (CML) cells and from the Chronic Phase (CP) Chronic Myeloid Leukemia (CML) cells.