GSM3580622: Mouse_term_myometrial_cell_4_RNAseq; Mus musculus; RNA-Seq
Flash frozen tissues stored in -80oC were homogenized in TRIzolTM reagent (Invitrogen) using a bead homogenizer (Fisherbrand™ Bead Mill 24) for two 20sec cycles at 5000 rpm. Near-confluent cells were lysed using 1mL TRIzolTM reagent. Total RNA was isolated per the Trizol's manufacturer instructions,  DNase-treated (DNA-free kit, Ambion) and then sent to the Vanderbilt Technologies for Advanced Genomics (VANTAGE) core for quality control and cDNA library preparation. The RNA was analyzed with an Agilent Bioanalyzer 2100, which showed that all samples had an RIN>8. The coding RNA was enriched using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (E7490) and paired end libraries were generated using the NEBNext Ultra II Directional RNA Library Kit for Illumina (7760).
GEO Accession
GSM3580622