GSM1486390: d0 CGN DNase-seq Rep 1; Mus musculus; DNase-Hypersensitivity
15-30 million nuclei were digested with a range of recombinant DNase I enzyme (Roche) concentrations (between 1.2 and 12U) for 15 minutes at 37°C in 120uL 1X DNase buffer. Digestions were checked by pulse field gel electrophoresis and material was pooled from 3 different DNase concentrations (extent of digestion matched between samples) in equimolar amounts following blunt-ending reactions. This was followed by ligation to adapters, MmeI digestion, streptavidin bead-based enrichment, 14 cycles of PCR amplification, and separation from linker-only bands on a gel. Cell pellets were washed twice with 1xPBS and nuclei isolated by 0.1% NP-40 lysis
GEO Accession
GSM1486390