PacBio DNA sequencing of a single pair of adult S. haematobium worms
Sequencing was performed on the PacBio RS II system (Pacific Biosciences, Menlo Park, CA) using 33 SMRT cells and Sequencing Reagent 2.0 (Pacific Biosciences, Menlo Park, CA). Data was collected for 180 minutes each with the stage start option. Upon acquisition of the sequencing data, adapters were removed and short reads (< 50 bases) and nucleotides with an estimated polymerase read quality value of less than 0.75 were removed using the SMRT analysis software (Pacific Biosciences, Menlo Park, CA).