GSM4256652: mock infected cells (DV), replicate 3; Homo sapiens; RNA-Seq
Total RNA was extracted with TRIzol reagent. After TURBO DNAse treatment of 30 min, a new phenol:chloroform extraction was performed and the extracted RNA was subsequently used to construct the libraries. Libraries were prepared using the TruSeq Stranded Total RNA Library Prep Kit  (ref. RS-122-2301) according to the manufacturer's protocol starting with 0,5 µg of total RNA for ribosomal RNA depletion. Libraries were analyzed with the Agilent Bioanalyzer using a DNA 1000 chip to estimate the quantity and check size distribution, and were then quantified by qPCR using the KAPA Library Quantification Kit (ref. KK4835, KapaBiosystems) prior to sequencing with 2x75 cycles of High output paired-end reads on Illumina's NextSeq with v4 sequencing chemistry.
GEO Accession
GSM4256652