GSM4699548: ATAC-seq, day 0 of EBV infection, donor 1; Homo sapiens; ATAC-seq
5 x 104 cells were washed in 50ul cold PBS, spun down at 500 x g and 4C for 5mins, and resuspended in 50ul cold lysis buffer (10mM Tris pH 7.5, 10mM NaCl, 3mM MgCl2, 0.1% IGEPAL CA-630).  Resuspended cells were immediately spun at 500 x g and 4C for 10mins, and the supernatant was removed.  The pellet was then resuspended in a 50ul Tn5 transposase reaction following the manufacturer's protocol (Nextera Tn5 transposase kit, Illumina) and incubated in a thermomixer at 37C and 300rpm for 30mins.  DNA was purified using a MinElute PCR purification kit (Qiagen) and eluted in 10ul to be used in library amplification PCR amplification of tagmented DNA was done using the NEBNext HiFi PCR mastermix (New England Bioloabs) with a universal forward and sample-specific reverse oligo for sample barcoding
GEO Accession
GSM4699548