Tonnersjoheden_forest_nitrogen_fertlized_mineral_soil_layer_plot_3_subreplicate_3
DNA was extracted from mesh bag sand samples by freeze-drying, dry bead beading, and extraction with CTAB-SDS, followed by chlorofrom wash and ethanol precipitation. DNA was cleaned up with Wizard DNA clean-up kit and amplified with ITS1F and ITS4 primer pair. PCR was conducted with 53oC annealing temp for 33 cycles, and PCR products were cleaned up with AMPURE and quantified with PicoGreen. Sequencing was done using Lib-L chemistry and sequencing started from the ITS4 fragment end.