GSM1480726: Dpu_bisulfite_rep3; Daphnia pulex; Bisulfite-Seq
Thirty 14-15 day old daphnids per biological replicate were pooled, shock-frozen in liquid nitrogen and stored at -83°C until DNA extraction. Extraction of the total genomic material was performed using the MasterPure kit (Epicentre, Madison, WI, USA) according to the manufacturer’s protocol, including RNAse treatment. Bisulfite library construction and sequencing were performed at BGI-Shenzhen (Shenzhen, Guangdong, China). Briefly, the extracted DNA was fragmented by sonication to a mean size of approximately 300 bp. After blunt ending and 3′-end addition of dA, Illumina methylated adapters (Illumina, San Diego, CA, USA) were added according to the manufacturer's instructions. The bisulfite conversion (C → U) was carried out using the EZ DNA methylation Gold kit (Zymo Research, Irvine, CA, USA) according to manufacturer’s instructions.
GEO Accession
GSM1480726