RNA-Seq of mus musculus: adult calf muslce
total RNA samples (0.2510 ng) were reverse-transcribed using random priming into double-stranded cDNA in the presence of a template switch oligo (TSO). Ribosomal cDNA was cleaved by ZapR in the presence of the mammalian-specific R-Probes. Remaining fragments were enriched with a second round of PCR amplification using primers designed to match Illumina adapters. The product is a smear with an average fragment size of approximately 360 bp. The libraries were normalized to 10nM in Tris-Cl 10 mM, pH8.5 with 0.1% Tween 20.