GSM4704052: Human multiple myeloma cells with NAT10 overexpression for ac4C-seq [C-OE]; Homo sapiens; RIP-Seq
RNA fragments were incubated with anti-N4-acetylcytidine (ac4C) antibodies immunoprecipitation (IP) buffer for 4h at 4℃. The samples were then washed with low-salt precipitation buffer and high-salt buffer thrice continuously. RNA was extracted using a phenol-chloroform lysate to obtain a purified product. The purified RNA was collected for the generation of an RNA-seq library with NEBNext R Ultra™ RNA Library Prep Kit (New England Biolabs, MA, USA). The quality of the library was done using Bioptic Qsep100 analyzer and sequencing was done using NovaSeq's high-throughput sequencing platform
GEO Accession
GSM4704052