Padlock Capture on coding regions of RNA transcripts of X-linked genes in 4-day differentiated 3F1 cells with RNA polymerase III inhibitor treatment during the first two days of in vitro differentiation
The cells were differentiated in vitro for four days. During the first two days of in vitro differentiation, the cells were treated with RNA Pol III inhibitor. Total RNA was isolated and cDNA was generated by reverse transcription using oligo (dT) primers. The cDNA sample was subjected to padlock SNP capture using a padlock probe library targeting SNPs selected from the coding regions of X-linked genes along the mouse X chromosome.