Illumina HiSeq 2500 paired end sequencing; RNA-seq of control siRNA, p150 siRNA and p60 siRNA transfected mES cells
RNA-seq of control siRNA, p150 siRNA and p60 siRNA transfected mES cells
Total RNA was extracted with GenElute total RNA miniprep kit, treated with turbo DNase and purified with RNeasy MinElute Cleanup kit. The quality was assessed on a bioanalyzer. ES cells were cultured in DMEM with GlutaMax (Life technologies) containing 15% FCS and LIF Libraries were prepared with Truseq Stranded Total RNA with Ribo-Zero Gold Prep kit (Illumina). Lipofectamine RNAi MAX (Life technologies) were used to transfect siRNA.
Experimental Factor: RNA interference
p60 siRNA