Pecten maximus transcriptome sequencing
Individuals in each treatment were sampled over a time course from the beginning of the experiment and then after 3, 7, 14, 21, 27, 42 and 56 days. The scallops were dissected and mantle tissue was flash frozen in liquid nitrogen and stored at -80°C until further analysis. Total RNA was extracted from mantle tissue of 4 individuals per treatment at each time point using TRI Reagent® Solution (Life Technologies) according to manufacturer’s instructions. RNA quality and concentration were determined using an Agilent 2100 RNA Nanochip (Agilent, Santa Clara, CA, USA) and a NanoDrop ND-1000 Spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA), respectively. For each condition, the RNAs from the 4 individuals at each time point were then pooled for RNA-Seq. From these pooled samples, 22 cDNA libraries were produced (cf. Table 1 for details). The production of the Illumina libraries and the transcriptome sequencing using the Illumina HiSeqTM2500 (HiSeq 100bp pair-ends) was conducted by the Genome Analysis Centre (Norwich, UK).