GSM3893604: B131T14; Homo sapiens; RNA-Seq
Bone marrow mononuclear cells were purified on Ficoll gradient and processed for nucleic acid extraction using the DNA/RNA Kit (Qiagen). cDNA synthesis was conducted with MuLV Reverse Transcriptase (Invitrogen), with quality controls conducted on an Agilent 2100 Bioanalyzer (Agilent). Libraries were constructed using the TruSeq Stranded mRNA Sample Preparation Kit (Illumina) and sequenced on an Illumina HiSeq 2500 platform using a 100-bp paired-end sequencing strategy.
GEO Accession
GSM3893604