The Positive Transcriptional Elongation Factor (P-TEFb) is required for Neural Crest Specification
library preparation. cDNA was end-repaired, A-tailed and adapter-ligated before amplification and size selection. The prepared libraries undergo quality control by running on a gel and using a bioanalyser to check library fragment size distribution before 100bp paired end sequencing over one lane of a flow cell. Samples were multiplexed 2 x 3 and ran on one lane each to yield approximately 25 million paired reads per sample.