GSM2193042: ZIP10 negative cells isolated from hair follicles of mice; Mus musculus; RNA-Seq
Ventral skins excised from 7-day-old C57BL/6 mice were minced with a scalpel and incubated overnight with Dispase II (2.4 U/ml, Roche), Collagenase (50 U/ml, Worthington Biochemical Corporation, Lakewood, NJ), and 10% FBS in DMEM (Invitrogen) at 4 ºC. Next, the tissues were pipetted vigorously to dissociate the cells, which were filtered through a 40-μm cell strainer (Greiner Bio-One) and incubated with anti-ZIP10 polyclonal antibody or normal rabbit IgG for 30 min on ice, and then with Alexa Fluor 488-conjugated anti-rabbit IgG for 15 min on ice. The cells were also stained with 7-AAD (BD), and ZIP10+ and ZIP10− cells were sorted with a FACSAria II (BD). The RNA-sequencing library was constructed from total RNA (10 ng) using a NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Inc., Ipswich, MA). The prepared libraries were sequenced using an Illumina HiSeq 1500 in 50-base single-end mode.
GEO Accession
GSM2193042