RNA-seq Rhazya stricta foliar transcriptome; Sample A8
All material was collected from a site at Bahrah near Jeddah in the Kingdom of Saudi Arabia Leaves were picked, wrapped in aluminium foil, labelled and snap frozen in liquid nitrogen and then stored at -80 *C until required for extraction.   Leaf tissue was ground to a fine powder in liquid nitrogen using a pestle and mortar, then approx 100mg of tissue was RNA extracted using Trizol* (Life technologies, Carlsbad, USA) as described by the manufacturer. The RNA was then purified using a Spin Column Reaction Cleanup Kit (NBS Biologicals, Huntingdon, UK). The RNA was then purified using a Spin Column Reaction Cleanup Kit (NBS Biologicals) as described by the manufacturer.  RNA samples were sequenced by Genome Enterprise Limited (GEL) at The Genome Analysis Centre (Norwich, UK) across 8 lanes of an Illumina HiSeq 2000 (Illumina, San Diego USA); using 50bp paired- end reads, insert length ~200bp. The data was de-multiplexed and quality checked using FastQC (www.bioinformatics.babraham.ac.uk/projects/fastqc/) by GEL.