dRNA-seq of UTEX2973, HL sample2, minus library
Total RNA was firstly fragmented by ultrasound, treated by Terminator-5'P-dependent-exonuclease (TEX) to enrich 5'PPP-containing primary transcripts, ligated with 5'-RNA-adapter. cDNA was synthesized by using oligo-dT primers containing 3'-RNA-adapter and PCR amplifications.