Myotis ricketti (1)
Experimental Design: To elucidate the intestinal bacterial and eukaryotes community, we performed 16S and 18S ribosomal RNA gene sequencing using 14 fecal samples from the intestinal tract of healthy bats across nectar-, fruit-, and insect-feeding. Library Construction:Total genomic DNA was subjected to PCR amplification using barcoded primer sets, which targeted the variable region 4 of bacterial 16S rRNA genes and variable region 9 of eukaryotic18S rRNA genes. After quantifying PCR products, we constructed PCR free library further to sequence amplicons using the Illumina Miseq sequencing platform.