NextSeq 500 paired end sequencing; DNA sequencing of Arabidopsis thaliana nrpd1-3 background with novel insertions of LTR retrotransposon ONSEN/COPIA78.
DNA sequencing of Arabidopsis thaliana nrpd1-3 background with novel insertions of LTR retrotransposon ONSEN/COPIA78.
Arabidopsis thaliana seeds were surfaced sterilized and sown in petri dishes with agarified (0.8% Bacto Agar) 0.5x MS medium, containing 1% sucrose and 0.05% MES at pH 5.7. After stratification for 3 days at 4°C, plants were grown in a CU-22L growth chamber (Percival) under 21°C and a 12/12 h (day/night) light cycle. Pools of 7 days old seedlings were harvest for DNA extraction. Genomic DNA was isolated using the DNeasy Plant Mini Kit (Quiagen), following provider's instructions  PCR-free libraries for genome sequencing were prepared with 1 µg of DNA using the TruSeq DNA PCR-Free Library Prep kit (Illumina); following provider's instructions.
Experimental Factor: individual
plant 5L2