RNA-seq of an embryonal rhabdomyosarcoma tumor
"After ribogreen quantification and quality control of Agilent BioAnalyzer, 1?g of total RNA (RIN between 5 and 7) underwent polyA selection and Truseq library preparation according to instructions provided by Illumina (TruSeq? RNA Sample Prep Kit v2), with 6 cycles of PCR. Samples were barcoded and sequenced using the TruSeq SBS Kit v3 (Illumina). ?"