GSM1191886: 1027; Homo sapiens; OTHER
The sample was sheared at an average length of 300 bp and labelled using the mTAG approach as described in the high-throughput mTAG-click protocol. Library preparation involving adenylation of 3’-ends, adaptor ligation and PCR amplification with unique index primers was performed as described in the Illumina Multiplexing Sample Preparation Oligonucleotide kit. The only changes in this library preparation protocol were that the samples were treated with 10 mM 2-mercaptoethanol for 10 min at room temperature prior to PCR amplification, and only 14 cycles of PCR were performed.
GEO Accession
GSM1191886