GSM3721142: RMN053; Mus musculus; RNA-Seq
Total RNA was extracted using Arcturus PicoPure RNA Isolation kit according to manufacturer's protocol. All mouse RNAs were analyzed on Agilent Bioanalyser for quality assessment. cDNA libraries were prepared using 2ng of total RNA and 1ul of a 1:50,000 dilution of ERCC RNA Spike in Controls (Ambion) using SMARTSeq v2 protocol described in Picelli et al., 2014 (Nature Protocols, volume 9, pages 171-181) except for the following modifications: 1. Use of 20µM TSO, 2. Use of 250pg of cDNA with 1/5 reaction of Illumina Nextera XT kit.
GEO Accession
GSM3721142