GSM2797335: WT rep2; Dictyostelium discoideum; RNA-Seq
Cells were centrifuged and wash and total RNA was extracted with standard Trizol protocol cDNA libraries were prepared using the TruSeq Sample Preparation v2 kit (Illumina) according to the manufacture's recomendations. Poly-A containing mRNA was purified from 1ug of total RNA, fragmented. Following fragmentation cDNA was synthesized. cDNA fragments were end repaired, and a single ‘A’ base was added (to the 3’ end of the blunt fragments), afterwards barcoded (indexed) adapters were ligated to each sample. The products were purified and enriched with a 15 cycles PCR to obtain the final cDNA libraries.
GEO Accession
GSM2797335