GSM2795543: NMD tissue, replicate 3; Drosophila melanogaster; RNA-Seq
Flies were transferred from their media briefly into empty vials and anesthetized using FlyNap® (Carolina®).  The flies were then submerged in a petri dish with 1 mL TRIzol® reagent, and their heads were pulled off with forceps in rapid succession.  The heads (N=20) were then immediately placed in 200 µL of TRI Reagent® in a 1.5mL microcentrifuge tube.  There were six biological replicates (tubes) for each media group, with each replicate/tube containing 20 heads.  The head tissue in each tube was then homogenized using an electric pestle.  RNA extraction and purification was performed using a Direct-zol™ RNA MicroPrep kit (Zymo Research).  The RNA was eluted with sterile water, after which purity and concentration were confirmed via 260/280 and 260/230 ratios measured using a NanoDrop® 2000c (Thermo Scientific).  N=6 per experimental condition. Libraries were prepared according to the specifications accompanying the Illumina TruSeq Stranded mRNA Library Prep Kit High Throughput (RS-122-2103).
GEO Accession
GSM2795543