RNA-seq on Campylobacter jejuni NCTC11168 grown in iron replete/limited conditions
Campylobacter jejuni NCTC11168 was grown either in iron replete ( 40 *M FeSO4) or iron limited (-40 *M FeSO4) MEMα. Total RNA was isolated as described previously using a hot-phenol chloroform method. RNA pellets were resuspended in TE buffer and treated with DNase I (Epicentre) to remove contaminating genomic DNA and PCR was performed to confirm the absence of DNA. Ribosomal RNAs were depleted using the RiboMinusTM transcriptome isolation kit from Life Technologies. Library construction and cluster generation was done using standard Illumina protocols with sequencing performed on an Illumina HiSeq 2000 sequencer.