ChIP-seq (H3K9me3) of Plasmodium falciparum: wild type 3hpi
Commercially available antibodies to H3K9me3 (Millipore) were added to cross-linked samples  and incubated at 4C, followed by the addition of 10 l A/G beads and further incubation for 2 h. After washing with buffers containing 100, 150 and 250 mM NaCl, immuno-precipitated DNA was eluted and purified using PCR purification columns (Qiagen). The resulting dsDNA was then end repaired, followed by adding an A base at the ends. Illumina paired-end index adaptor was ligated and size selected. A 16-cycle PCR was then carried out with Phusion Hot Start High-Fidelity DNA Polymerase (Finnzymes) to generate the final ChIP-seq library.