GSM4649229: KN3501α_Cysteine; Cryptococcus neoformans var. neoformans; RNA-Seq
Total RNAs were purified using the Fast Gene Premium Kit (Nippon-Genetics) according the protocol provided by the manufacturer. The purity of the RNA was measured on a NanoDrop 2000 instrument (Thermo Fisher). The RNA levels and integrity were determined using the RiboGreen RNA Assay Kit on a Victor X2 Fluorometer (Life Technologies, USA) and the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, USA), respectively. One library was prepared using the TruSeq Stranded mRNA Library Prep Kit (Illumina), and cDNA was sequenced on an Illumina NovaSeq 6000 instrument in a 2 ´ 150 paired-end protocol.
GEO Accession
GSM4649229