GSM3578654: C8B21; Homo sapiens; RNA-Seq
Embryos were treated with trypsin to detach from dishes. Embryos then be and manually transferred to a new dish containing trypsin for further dissociation for 3 to 5 minutes with repeated aspiration using a mouth-controlled, finely drawn capillary pipette. Single cell was then manually picked using the capillary pipette into a 0.2-ml thin-wall PCR tube contains lysis buﬀer. Library construction is done using SMART-seq2 protocol with minor modification. Single cell RNA sequencing
GEO Accession
GSM3578654