RNA-seq of Plasmodium falciparum: Pf3D7G7 (without drug) 10hpi
Both nascent RNA-seq library and steady-state RNA-seq library were prepared using KAPA Stranded RNA-seq Kit with RiboErase (HMR) (KR1151, KAPA Biosystems) according to the manufacturers instructions except the PCR application steps. Because of AT-rich genome of P. falciparum, libraries were amplified for 14 cycles using the following conditions: 45 s at 98  for initial denaturation14 cycles of (15 s at 98  for denaturation, 30 s at 60  for annealing and 30 s at 65  for extension) and 5 min at 65  for final extension. All purified libraries were sequenced on Illumina HiSeq X Ten (Illumina, San Diego, CA, USA) to acquire 150 bp paired-end sequence reads.