GSM2796624: 48 hpf control MO-injected sample A; Danio rerio; RNA-Seq
TRIzol Reagent RNA Extraction protocol (Ambion RNA Life technologies) was used to extract RNA from pooled samples of hace1 or control morphants (n=6 per group).  Approximately 100 embryos per sample were used.  Tubingen wild type strain (TU) fish were previously injected with either hace1 MO or control MO using the injection protocol noted above.  RNA samples were quantified using the NanoDrop Spectrophotometer ND-1000 (Thermo Fisher Scientific, Wilmington, DE).  RNA yields for this experiment were lower than the 100µg of RNA needed for RNA sequencing, so 3 samples were pooled into one, for a final n=2 for each of the hace1 MO and control MO groups. Barcoded cDNA libraries were prepared using RNA-Seq Version 2 kit from Life Technologies following their recommended protocol. Library preparations were assayed for both quality control and quantity using Experion DNA 1K chip (Life Technologies) and diluted to 16 pM concentration. Samples were sequenced using a Proton Sequencer from Life Technologies on a PI chip following the manufacturer recommended protocol. Each chip was loaded with 2 samples.
GEO Accession
GSM2796624