Neogobius melanostomus microsatellite sequences: fin tissues and eDNA samples
Microsatellite loci were amplified from eDNA and round goby  tissue samples in separate reactions using multiplex PCR methods. Products from all multiplexes were pooled and uniquely barcoded in a second-stage PCR using Illumina Nextera XT tags. One library was constructed from the pooled PCR products for all tissue and eDNA samples in the mesocosm experiment, while another library was constructed from the tissue and eDNA samples from the field trial. DNA libraries were purified with Agencourt AMPure XP beads and the concentration of each library was estimated using the Qubit dsDNA High-Sensitivity Kit and Qubit 2.0 fluorometer. The libraries were diluted to 4 nM with PCR-grade water and paired-end sequenced on an Illumina MiSeq sequencing platform (Illumina, San Diego, CA) with the MiSeq v2 500 bp kit (PE 2 x 250 bp) by Cornell Universitys Institute of Biotechnology Genomics Facility.