WGS sequencing of  Sphenodon punctatus
High-molecular-weight  genomic DNA was extracted using the Qiagen Genomic Tip DNA extraction kit (genomic-tip column 100/G) from ~20mg  of liver tissue according to the manufacturer's  directions, and was performed at the Laboratories  of Analytical Biology at the Smithsonian Institutions National Museum of  Natural History (NMNH). A PacBio SMRTbell library was prepared using the  SMRTBell Express Template Preparation Kit (Pacific Biosciences) following the  PacBio 15-kb template preparation protocol, with a minimum size cutoff of  5kb size selection used in the size selection stage with the Sage Science  BluePippin system.The prepared library was run on the PacBio Sequel  platform (Pacific Biosciences) using version 2.1 chemistry. The Single  Molecule Real-Time (SMRT) cell was sequenced with 360min movie lengths.