RNA-Seq of different pathogen E. coli in lambs lettuce medium
The RNA quality was determined with an Agilent 2100 Bioanalyzer and samples with a high RNA integrity number (RIN > 8) were selected for library construction. Per replicate, a total amount of 1.6 g RNA was subjected to rRNA depletion using a Ribo-Zero rRNA Removal Kit Bacteria (Illumina) according to the manufacturers instructions. The cDNA libraries were constructed using the resulting RNA and the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) according to the manufacturers instructions.