GSM1195325: footprint 0-2 hour embryo gradient replicate B; Drosophila melanogaster; OTHER
Samples were lysed using the indicated strategies in the indicated buffers. For ribosome profiling samples, monosomes were resolved by digestion with micrococcal nuclease,  isolated by sucrose cushions or gradients as indicated and  RNA was isolated from monosome fractions using acid:Phenol and chloroform extractions. For RNA-seq samples, RNA was extracted with Trizol For RNA-seq, poly(A)+ RNA was selected on oligo dT(25) dynabeads, randomly fragmented to approximately 50 nt in length, and size-selected to 40-60mers via gel purification. For ribosome profiling, 28-34mers were size-selected via gel purification. All fragments were then dephosphorylated, ligated, and reverse transcribed. The resulting cDNA was circulizarized, PCR amplified, and subjected to 50-57 cycles of sequencing on an Illumina HiSeq, as indicated
GEO Accession
GSM1195325