GSM3580209: Set2_F234Y_60_RNA_Rep3; Saccharomyces cerevisiae; RNA-Seq
Ten ODs of cells were collected at each time point and RNA was isolated by acid phenol extraction. Five μg RNA was treated with DNase (Promega) and purified (RNeasy column, QIAGEN). Two and a half μg RNA was then processed using yeast-specific rRNA depletion beads (Illumina). Strand-specific bar-coded sequencing libraries were then prepared (TruSeq Stranded Total RNA Library Preparation Kit, Illumina). Libraries were pooled and sequenced across two lanes (Hi-Seq 4000, Illumina).
GEO Accession
GSM3580209