GSM2871009: siblings replicate 3; Danio rerio; RNA-Seq
20 larvae were sampled and homogenized with micropestles (Eppendorf, #Z317314-1PAK) in 900 µl Quizol reagent before being stored overnight at -80°C. Samples were passed several times through a syringe (BD Microlance, 0.5 x 25 mm, #3086982), and RNA was extracted using the RNeasy Plus  Universal Kit (Qiagen, #73442) according to the manufacturer's instructions. The integrity of the total RNA was checked on an agarose gel and with a NanoDrop spectrometer (Thermo Scientific). Only RNA with A260/280 values ≥2 and A260/230 values ≥1 were used for the subsequent analysis. Library prep for total RNA Ribozero
GEO Accession
GSM2871009