GSM2527101: Cyo_200_High_rep1; Escherichia coli BW25113; OTHER
Sorted cells were pelleted, and genomic DNA was purified using the Qiagen DNeasy Blood and Tissue Kit. Barcodes were amplified as described in Wetmore (2015). In brief, barcodes were amplified using Q5 DNA polymerase with a common primer and indexed primer. Amplicons were checked with gel electrophoresis and purified using the Zymo Clean and Concentrator kit. Finally, concentrations were measured with Qubit HS assay, and samples were multiplexed.
GEO Accession
GSM2527101