GSM3405919: ATAC-seq, AT-hMSC-TERT undifferentiated rep1; Homo sapiens; ATAC-seq
ATAC-seq: Cells were scrabed off from 6-well dishes in ice cold PBS, centrifuged and permeabilised. Nuclei were rinsed through a cell strainer (10 micro m) and counted. 5.000 nuclei were exposed to 1 micro l transposase as described originally (Buenrostro J D, et. al 2015. Current Protocols in Molecular Biology 109: 1-9). ATAC-seq libraries were constructed according to the manufacturer's instructions (Illumina).
GEO Accession
GSM3405919