Illumina MiSeq paired end sequencing; Small RNA discovery in developing mouse and during human in vitro beta-cell differentiation protocol
Small RNA discovery in developing mouse and during human in vitro beta-cell differentiation protocol
See Hrvatin et al. 10.1073/pnas.1400709111 SI Experimental Procedures. Small RNA's were size fractionated from total RNA based on charge. Capped transcripts were enriched by immunoprecipitation and poly-A selected with Oligo dT. An enzymatic cocktail was applied to modify the RNA's for use with the NEBNext Kit. Please contact the investigators for further information. NA
Experimental Factor: organism part
not applicable
Experimental Factor: stage
stage 1