GSM3579698: Control ATAC-seq 3; Homo sapiens; ATAC-seq
Neurons were scraped from the plate and nuclei were isolated using hypotonic buffer (10 mM Tris-HCl pH7.4, 10 mM NaCl, 3mM MgCl2, 0.1% [v/v] Igepal CA-630) and counted. 50,000 nuclei were resuspended in 50 µl transposition reaction mix containing 2.5 µl Nextera Tn5 Transposase and 2x TD Nextera reaction buffer. The reaction was incubated for 30 min at 37 ºC. DNA was purified by either MinElute PCR kit (Qiagen) or Agencourt AMPure XP (Beckman Coulter) beads and PCR amplified with NEBNext High Fidelity reaction mix (NEB) to generate a sequencing library.
GEO Accession
GSM3579698