16S rRNA amplicon sequence - soil microbial community
DNA was extracted from portions of 250 mg of homogenized ground soil using the MoBio PowerSoil DNA Isolation Kit.Extracted DNA was amplified using the 16S rRNA gene primer pair 515-F (5-GTGCCAGCMGCCGCGGTAA-3) and 806-R (5-GGACTACHVGGGTWTCTAAT-3), which included Illumina adapters and unique barcode sequences for each sample.