GSM1354549: U2932_RNAPII; Homo sapiens; ChIP-Seq
U2932 cells were collected, cross-linked, sonicated and lyzed. Samples were immunoprecipitated with polyclonal antibodies specific for PRDM11, RNA polII (sc-899X, Santa Cruz) or rabbit IgG (Sigma). Eluted DNA was de-crosslinked and extracted using the QIAquick PCR Purification Kit (Qiagen) or MinElute PCR purification Kit (Qiagen) DNA libraries were build using NEBNext DNA Library Prep (NEB) according to manufacturer’s instructions
GEO Accession
GSM1354549