GSM2194298: ABAF490 16152; Homo sapiens; RNA-Seq
Islets were dissociated to single cell by 0.05% Trypsin, 9 mins at 37oC. Cells (250-500 cells/μl) were subsequently loaded on Fluidigm C1 IFC with either 5-10 μM or 10-17 μM capture size. Smarter-seq V2 kit was utilized for library construction per manufacturer's protocol to generate cDNA.  Prepration of Illumina libraries from cDNA was done with the NexteraXT kit per Fluidigm’s instructions.
GEO Accession
GSM2194298