GSM3405620: smpB1; Aeromonas veronii; RNA-Seq
Bacteria were collected by  refrigerated centrifuger and lysised by lysozyme. RNA samples were extracted by phenol-chloroform.The concentration and quanlity were  detected by Alilent 2100 Bioanalyzer The samples were treated with Dnase I to degrade double-stranded and single-stranded DNA. And then the rRNA was depleted by Ribo-Zero Magnetic Kit. RNA moleculess were fragmented into small peces.First-strand cDNA was generated using random primers reverse transcription, followed by a second-strand cDNA synthesis.The synthesized cDNA was subjected to end-repair and then was 3' adenylated. Adapters were ligated to the ends of these 3' adenylated cDNA fragments.The cDNA fragments was inriched  by PCR amplification with PCR Primer Cocktail, and the product were purified and ready for sequencing.
GEO Accession
GSM3405620