GSM1199554: Strand Specific, Paired end NanoCage analysis of Total RNA from Mouse Ter119+ erythroid  cells; Mus musculus; RNA-Seq
RNA was isolated with Tri-Reagent (Sigma). The RNA quality was assessed using Agilent Bioanalyser. Only RNA with overall RIN score >9 was used for further procedures. Subsequently, RNA was treated with DNAseI (DNA-free, Ambion) according to the manufacturer’s protocol kit according to the protocol.  The RNA fraction was precipitated overnight and resuspended with water. The quality of obtained RNA samples was assessed using PicoChip (Agilent). A Paired-End Nancage library was commercially generated via Source Bioscience. The library was constructed commericailly via the company Source Bioscience as laid out in the publication "NanoCAGE: A High-Resolution Technique to Discover and Interrogate Cell Transcriptomes" Salimullah et al Cold Spring Harb. Protoc. 2011
GEO Accession
GSM1199554