GSM3268046: DSS-rep1; Mus musculus; RNA-Seq
Total RNA was extracted by TRIZOL reagent according to the manufacturer's instructions (Invitrogen, USA). The quality and quantity of total RNA were assesses using 2% agarose gels and Qubit® 3.0 fluurometer (Life Technologies, CA, USA). The concentration and integrity of RNA were assessed using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB E7490) and NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module (NEB E6111). RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM3268046