Transcriptome of developing culms in Moso bamboo
Tissue samples from 18 culms were collected for RNA preparation. Total RNA was isolated using a TRIzol reagent (Gibco BRL) following the manufacturer's instructions, and then beads with Oligo (dT) were used to isolate poly (A) mRNA. After fragmentation, random hexamer-primer was used to synthesize the cDNA. Short cDNA fragments were purified and followed by end reparation, adenylation and sequencing adapter ligation. After PCR amplification of the selected fragment, the library was sequenced using Illumina HiSeq 2000.