GSM3351158: Normal Endothelial Cells (NEC); Mus musculus; RNA-Seq
Normal tissues or tumors were harvested and dispersed into single cell suspensions in a collagenase/dispase/DNAse cocktail according to our published methods (Xiao et al. Cancer Research 2014). After filtering and washing the suspensions, samples were subjected to FACS (fluorescence activated cell sorting) to collect live ZSGreen+ cells from each fraction (a live/dead stain was added to exclude dead cells). The collected fractions were prepared for analysis using the 10X genomics platform. Libraries were prepared for sequencing on the 10X chromium according to the manufacturer's instructions for the single cell 3' reagent kit.
GEO Accession
GSM3351158