RNA-Seq of Xanthomonas cucurbitae: hrp-inducing media
RNA was extracted using E.Z.N.A. total RNA kit from Xanthomonas cucurbitae grown on hrp-inducing media 24 hours prior to harvesting. Ribosomal RNA was removed with the Ribo-Zero rRNA Removal Kit for Gram-Negative Bacteria and libraries were prepared using the TruSeq Stranded mRNA Library Prep (Illumina). Paired-end 250 bp reads were sequenced using the MiSeq Nano platform.