APMV-8 random PCR with primer FR20RV-6N, fragmented RNA
An avian paramyxovirus type 8 (APMV-8) isolate was centrifuged, filtrated and nuclease treated to enrich for virions. After viral RNA extraction, the RNA was fragmented. Subsequently sequence independent primer amplification (SISPA) was performed with random SISPA primer FR20RV-6N. This primer is composed of a random 6N oligomer tagged with a known sequence.  Purified and size selected (400-1200nt) random PCR amplicons were used as input for the sequencing library.