H3K9ac ChIP-Seq analysis of paracentral neural stem and progenitor cells from HuFNSC-T (A02879)
H3K9ac ChIP-Seq analysis of paracentral neural stem and progenitor cells from HuFNSC-T (A02879) using Illumina Genome Analyzer IIx
Refer to document 'REMC ChIP and Library Construction Protocol' from the Farnham Lab at the Roadmap Epigenomics Project site, Experimental Protocols page (URL: http://www.roadmapepigenomics.org/protocols/type/experimental/)
EXPERIMENT_TYPE
Histone H3K9ac
EXTRACTION_PROTOCOL
Refer to document 'REMC ChIP and Library Construction Protocol' from the Farnham Lab at the Roadmap Epigenomics Project site, Experimental Protocols page (URL: http://www.roadmapepigenomics.org/protocols/type/experimental/)
EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR
Bioruptor
EXTRACTION_PROTOCOL_SONICATION_CYCLES
30 min
CHIP_PROTOCOL
Refer to document 'REMC ChIP and Library Construction Protocol' from the Farnham Lab at the Roadmap Epigenomics Project site, Experimental Protocols page (URL: http://www.roadmapepigenomics.org/protocols/type/experimental/)
CHIP_PROTOCOL_CHROMATIN_AMOUNT
5 ug
SIZE_FRACTION
75-354 bp
CHIP_PROTOCOL_BEAD_TYPE
Protein G Mag Beads
CHIP_PROTOCOL_BEAD_AMOUNT
15 ul
CHIP_PROTOCOL_ANTIBODY_AMOUNT
10 ul
CHIP_ANTIBODY
H3K9ac
CHIP_ANTIBODY_PROVIDER
Cell Signaling Technology
CHIP_ANTIBODY_CATALOG
9649S
CHIP_ANTIBODY_LOT
1