WGS of Oncopig HCC Tumor Biopsy
DNA was extracted from Oncopig tissues and cell lines using the DNeasy Blood & Tissue Kit (Qiagen) following the manufacturers instructions. DNA (300 ng) was used to develop shotgun genomic libraries using the Hyper Library Construction Kit from Kapa Biosystems (Roche, Indianapolis, IN) by the Roy J. Carver Biotechnology Center (University of Illinois, Urbana IL) following standard protocols. The libraries were quantitated by qPCR and sequenced on two lanes for 151 cycles from each end of the fragments on a NovaSeq 6000 using a NovaSeq S4 reagent kit. Fastq files were generated and demultiplexed with with the bcl2fastq v2.20 Conversion Software (Illumina).