GSM3579165: Contusion SCI, Replicate 1; Rattus norvegicus; RNA-Seq
Animals were perfused with PBS supplemented with 20% RNAlater (Ambion, Life Technologies). T11-L4 bilateral DRGs were retrieved from both sides of the spinal column and collected in QIAzol (Qiagen). Total RNA from each sample was isolated with RNeasy Lipid Tissue Mini Kit (Qiagen). Libraries were prepared using the TruSeq Stranded mRNA LT Sample Prep Kit- Set A (Cat# RS-122-2101) and Set B (Cat# RS-122-2102) with poly-A enrichment.
GEO Accession
GSM3579165