GSM3659982: MB 3 (scRNA-seq); Mus musculus; RNA-Seq
Fresh tumor tissues were isolated macroscopically and minced using scalpels prior to enzymatic dissociation for 20 minutes at 37°C using PBS-diluted Accutase, accompanied by mechanical dissociation and followed by the lysis of red blood cells using ACK lysis buffer. Cells were stained with the dye 7-AAD for cell sorting to exclude dead cells. 10000 vital cells per sample were loaded as input on the 10x Genomics Chromium controller. Indexed-single cell RNA libraries were prepared for sequencing using the 10X Chromium Single Cell 3' Gel Bead Kit and Chromium i7 Multiplex Kit (10x Genomics, PN-120262). Each sample was sequenced across 4 lanes with Illumina NextSeq 500 System (2 x 75bp). For sample 1, we sequenced the same library at to subsequent time points, to improve saturation.
GEO Accession
GSM3659982