GSM3721820: LncMyoD Knockdown Rep 1; Mus musculus; RNA-Seq
Medium was removed, and total RNA was isolated using NucleoSpin RNA XS (Macherey-Nagel). cDNA was generated following the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech) protocol. The RNA-Seq libraries were sheared with a Covaris S220 and generated using Ovation Ultralow DR Multiplex System 9-16 (NuGEN Technologies). The libraries were sequenced using Illumina NextSeq 500 (2 x 150).
GEO Accession
GSM3721820