PWSC-3
Total RNA was extracted using RNeasy Mini Plus kit from Qiagen using the Qiashredder column. Extracted RNA was checked for quantity and quality using an Agilent Bioanalyzer Model 2100. Gene libraries were prepared using the Kapa Biosystems stranded mRNA-seq kit, and sequenced using an Illumina NextSeq instrument using the Mid Output Flow Cell (300 cycles) 150 bp paired-end.