GSM3405902: cordBlood_TNrev_rep4 [8-409-Rev]; Homo sapiens; ATAC-seq
T cells sorted on Aria Fusion (Becton Dickinson, New Jersey, USA)  were  pelleted and resuspended in a 50-μl reaction cocktail containing 1 μl of 0.5% Digitonin (Promega, Madison, WI, USA), 2.5 μl of Tn5 transposase and 25 μl of TD buffer (Nextera DNA library preparation kit, Illumina, San Diego, CA, USA) and incubated for 30 min at 37°C with gentle shaking. The tagmented DNA was then purified using Minielute PCR Purification kit (Qiagen) and eluted into 11 μl of elution buffer. Transposed DNA was then amplified using  2.5 μl of indexing primers and 25 μl of Nextera PCR master mix according to the PCR protocol previously described (Buenrostro, 2013, Nat. Methods). PCR clean-up was then performed using AMPure XP beads (Beckman Coulter, Brea, CA, USA), and resuspended in 30 μl of resuspension buffer. The libraries were quantified using Qubit fluorometer (Life Technologies), and the size was detemined using TapeStation (Agilent).
GEO Accession
GSM3405902