GSM1356324: Pm nitrate-limited C; Pseudo-nitzschia multiseries; RNA-Seq
RNA was extracted from frozen cells on filters using the Totally RNA extraction kit (Life Technologies, New York, USA). The RNA was incubated with DNase I (Ambion, New York, USA) at 37°C for 2 hr and purified by DNase inactivation reagent (Ambion). Messenger RNA was polyA-selected from total RNA using the Ambion Micro Poly(A)Purist Kit (Life Technologies, New York, USA) to minimize bacterial RNA sequencing. The mRNA was then prepared for sequencing according to the SOLiD Total RNA-Seq Kit protocol. For each sample, 700 million beads were loaded onto a slide and run on a Life Technologies (New York, USA) SOLiD sequencer version 4.
GEO Accession
GSM1356324