10x WGS genome sequencing of the red palm weevil Rhynchophorus ferrugineus (barcoded reads)
DNA was extracted from a 4-8 mg section of an individual larva of Rhyncophorus ferrugineus following the 10x Genomics protocol for single insect DNA purification. Purified DNA was size selected to remove fragments smaller than 20kb using the BluePippin instrument. The resulting fragments were purified and loaded into the 10x Genome chip for barcoding. Barcoded DNA fragments were used to generate an Illumina paired-end sequencing library and sequenced on a NextSeq 500 PE150 mid-output flowcell.