GSM3049595: HCT116-HiChIP-SMC1; Homo sapiens; OTHER
HiChIP was performed essentially as described (Mumbach et al., 2016). 10 million HCT116 cells were crosslinked, nuclei were isolated and DNA was digested with MboI. Ends were filled in and proximity ligation was performed in situ before the nuclei were lysed and DNA was sonicated. Sonicated DNA was precipitated with SMC1A antibody and DNA was washed and purified. Sequencing libraries then were generated using transposase and the Nextera kit from Illumina. sonication
GEO Accession
GSM3049595