Whole shotgun metagenome sequencing of Room 1 New Plumbing biofilm sample=4
Microbiome DNA was extracted using the Qiagen DNeasy PowerSoil kit which involves mechanical bacterial lysis and a spin column to wash the DNA. Extracted DNA was fragmented and tagged with sequencing adapters using the Nextera XT library preparation kit, followed by paired-end sequencing up to read length of 150bp on Illumina HiSeq4000 platform