GSM4385428: ErbB3_V943R_1; Mus musculus; RNA-Seq
The thoracic and abdominal mammary glands from 6-week old ErbB3+/+;MMTV-Cre or ErbB3V943R/V943R;MMTV-Cre C57BL/6N mice (n = 3 per group) were pooled, minced, and collagenase-digested (Stem Cell Technologies) with constant agitation for 1 h.  The samples were further dissociated using an AutoMACS, followed by treatment with Trypsin-EDTA and DNAse I (StemCell Technologies) according to the manufacturer's recommendations.  Epithelial populations were enriched using the EasySepTM mouse epithelial cell enrichment kit II (StemCell Technologies), and then FACS sorted for luminal epithelial populations.  Post-sorting, the density and viability of the single-cell suspensions was determined by Vi-CELL XR cell counter (Beckman Coulter), and the cell density was then used to impute the volume of single-cell suspension needed in the reverse transcription master mix, aiming to achieve ~6,000 cells per sample. 10x Genomics Chromium Single Cell 3' Library and Gel Bead Kit v2
GEO Accession
GSM4385428