RNAseq of E coli: wild type stationary phase
E. coli BW25113 cells were isolated for RNA analysis after 8 hours of growth (~OD of 3.0).  Bacteria were collected by centrifugation (1 minute, 5,000 RPM) and pellets were stored at -70oC for up to one week prior to RNA isolation.  Total RNA from 4 mL bacterial pellets was isolated using the MasterPure RNA Isolation Kit.  RNA quality was assessed on an Agilent 2100 Bioanalyzer. DNase-treated total RNA was sent to the University at Buffalo Genomics and Bioinformatics Core for RNA-seq analysis (Buffalo, NY).  Ribosomal RNA was not removed prior to analysis.