GSM3351294: Caki2_shCTL_5hMethylCap-seq; Homo sapiens; OTHER
Tissue and cell line DNA were processed by DNeasy Blood & Tissue kit (Qiagen) according to manufacturer's requirement. Total DNA was extracted from urine samples using ZR Urine DNA Isolation Kit (Zymo Research). The DNA samples were sheared by sonication using the Covaris S220 (COVARIS, INC.) to achieve an averaged DNA fragment size around 300 bp. The immunoprecipitation was performed using the Methylated DNA Capture Kit (Diagenode) and the IP-Star Compact Automated System (Diagenode). The libraries were generated using PrepX ILM DNA Library Kit (Illumina) per manufacturer's instructions. Library quantification is performed using Qubit (Invitrogen) and Labchip GX HS (PerkinElmer) system for library fragment analysis. The libraries were subjected to massively parallel sequencing using HiSeq 2500 (Illumina) platform with the single-end 50 bp read option, according to the manufacturer's instructions.
GEO Accession
GSM3351294