RNAseq of Photorhabdus asymbiotica temperature response
rRNA was depleted from RNA samples obtained from exponential-phase cultures  using the Ribozero kit for Gram-negative bacteria (Epicentre), and verified using   a 2100 Bioanalyzer with a RNA 6000 Pico kit (Agilent). Strand specific RNAseq   libraries were constructed using the Illumina compatible ScriptSeq mRNA-seq library   preparation kit (Epicentre). cDNA libraries were quantified using a 2100 Bioanalyzer   with a DNA 1000 kit (Agilent). The first replicate of each library was sequenced   in two separate lanes on the Genome Analyzer IIx (Illumina), with 36 bp paired- end reads at the University of Exeter Sequencing Service.