GSM2194179: H2-26-act; Streptomyces albus; RNA-Seq
Mycelium was harvested by centrifugation. Briefly, ribosomal RNA depletion of isolated total RNA was performed with a Ribo-Zero Bacteria kit from Epicentre (Madison, WI, USA). The rRNA-depleted RNA was fragmented with RNase III, and 5′PPP structures were removed using RNA 5′ polyphosphatase (Epicentre). Next, RNA fragments were poly(A)-tailed using poly(A) polymerase. First-strand cDNA synthesis was performed using an oligo(dT)-adapter primer and M-MLV reverse transcriptase. The resulting cDNA was amplified and purified, and fragments in the length range of 250 – 500 bp were sequenced on an Illumina HiSeq 2000 machine (San Diego, CA, USA) with 100 bp read length.
GEO Accession
GSM2194179