Non-affected skin tissue from rainbow trout (fish 15) displaying puffy skin disease
The aim of this study was to test the hypothesis that Puffy Skin Disease (PSD) has an infectious aetiology, by undertaking a cohabitation experiment whereby naive triploid and diploid rainbow trout were cohabited with PSD-affected fish collected from the field. RNA was isolated from affected and non-affected skin samples and double-stranded cDNA (ds cDNA) was generated using Sequenase mix (Affymetrix, UK). DNA sequencing libraries were prepared using Illumina Nextera XT kits (Illumina, UK) and sequenced on an Illumina MiSeq (using the 2x150bp paired-end protocol) at the Animal Health and Veterinary Laboratory Agency (AHVLA, UK).