GSM1836472: aortic valve from high cholesterol model, replicate 2; Mus musculus; RNA-Seq
Total RNA from aortic valves was extracted using the Trizol method and purified using the Qiagen RNeasy column.  A DNase digestion was performed to remove genomic DNA. The Nugen Ovation RNA-Seq System v2 (San Carlos, CA) was used to generate cDNA from 1 ng of total RNA by using the manufacturer's suggested protocol. Indexed sequencing libraries were generated with the KAPA hyper prep kit  (Wilmington, MA) according to the manufacturer's suggested protocol.
GEO Accession
GSM1836472