Exome-Seq of Pinus sylvestris: haploid megagemetophyte
DNA extracted from haploid megagametophyte tissue. DNA fragmeted to 200 nt length with Bioruptor Ultrasonicator (Diagenode). Library prep with NEBNext DNA Library Prep Master Mix Set for Illumina and multiplexing with NEBNext Multiplex Oligos for Illumina E7600S (New England BioLabs). Targeted capture with MycroArray MYbaits protocol v.2.3.1.