GSM1479831: 5hmc-MeDIP-MCF7-hypoxia; Homo sapiens; MeDIP-Seq
genomic DNA was prepared with genomic DNA extraction kit (Promega) and sonicated into fragments ranging ~500bp. Libraries were prepared according to Illumina's instructions.  Illumina barcode adapters were ligated before hMeDIP. Adaptor-ligated DNA was denatured, followed by incubation with 5-hmC antibody (Active Motif). The immunoprecipitated DNA was purified and sequenced followed by standard Illumina protocols.
GEO Accession
GSM1479831