Illumina HiSeq 2500 paired end sequencing; Deep RNA Sequencing of native mouse podocytes and non-podocytes glomerular cells
Deep RNA Sequencing of native mouse podocytes and non-podocytes glomerular cells
Glomeruli were isolated using the beads perfusion method and glomerular singe cell solution was prepared as previously described (see Boerries M et al., Kidney Int. 2013 Jun;83(6):1052-64: Materials and Methods → Glomerular preparation & Podocyte preparation). Glomeruli were isolated using the beads perfusion method and glomerular singe cell solution was prepared as previously described (see Boerries M et al., Kidney Int. 2013 Jun;83(6):1052-64: Materials and Methods → Glomerular preparation & Podocyte preparation). RNA was extracted using the chloroform/phenol method with DNAase digestion (see Boerries M et al., Kidney Int. 2013 Jun;83(6):1052-64: Materials and Methods → RNA preparation).  The Illumina TruSeq stranded total RNA sample preparation LS protocol was used for directional, polyA+ library preparation.
Experimental Factor: Podocytes
cell type