GSM2526961: v966m-v966m_3; Drosophila melanogaster; RNA-Seq
Whole female larvae were collected and total RNA extracted with TRizol (Invitrogen). Polyadenylated RNA and ribosomal (rRNA)-depleted RNA was purified from total RNA using the MicroPoly(A)Purist Kit (Ambion) and the RiboMinus Eukaryote Kit for RNA-seq (Invitrogen), respectively. Sequencing libraries were prepared from Poly(A)+ and rRNA depleted RNA samples according to the manufacturer’s protocol (Illumina). All samples were sequenced with HiSeq2500 (Illumina) at the NIDDK Genomics Core Facility by 50 bp single-end sequencing.
GEO Accession
GSM2526961