Bacterial 16S rRNA gene (V1-V3) 454 pyrosequencing of methane-generating microbial communities
Triplicate PCR was prepared for each sample and the amplicons were pooled and purified using the Agencourt AMPure XP - PCR Purification Kit. After purification, the pooled amplicons were quantified using the Quant-iT Broad-Range DNA Assay Kit and equimolar concentrations of amplicons from each sample were mixed and sequenced on a Roche 454 GS FLX Titanium platform.