GSM3270913: P7ko3; Mus musculus; RNA-Seq
Brain cortices were removed, snap frozen in liquid nitrogen, homogenized and subjected to immunoprecipitation with anti-HA beads. Polyribosome-bound RNA was extracted using RNEasy Micro Plus Kit (Qiagen) and assessed for quality in BioAnalyzer 2000 (Agilent). 100 ng of RNA were used for sequencing library preparation with the TruSeq Stranded Total RNA Library Prep Kit (Illumina). Libraries were validated in the 2100 BioAnalyzer and quantified by qPCR and fluorometric analysis (Qubit 2.0, Invitrogen).
GEO Accession
GSM3270913