Sequencing of genomic DNA of Drosophila melanogaster Dam-expressing strain
High molecular-weight genomic DNA was isolated from the F1 offspring of the  [hsp70-FLP] x [STOP#1-Dam] crossed flies according to the standard procedure (Maniatis et al, 1982). Paired-end library of the fragmented genomic DNA (~250 bp fragment size) was prepared according to the Illumina standard protocol and sequenced on the Illumina HiSeq 2000.