GSM4101726: 18510_T; Homo sapiens; RNA-Seq
The nuclear RNA was extracted using the first part of the nuclear isolation protocl from Mayer and Churchman. Once the sampes were pelleted in the nuclear wash buffer we resuspended in 700 ul of QIAzol lysis reagent and RNA isolation was complted using an miRNAEasy kit (Quiagen). For each cell line we sampled both the total RNA in the cell, and the nucear RNA fraction through the ioslated nucleus. 3' RNA seq
GEO Accession
GSM4101726