GSM4649209: mouse-cerebellum granule cell-WT-RIP-Ythdf1-rep1; Mus musculus; RIP-Seq
Cerebellum were dissected and dissociated with trypsin, then cells were treated following the manual of the EZ-Magna RIPTM RNA-Binding Protein Immunoprecipitation Kit (Millipore) with minor modifications. RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. 100 ng RNA of input and elutes after RIP were used to generate the library using the TruSeq Stranded RNA Sample Preparation Kit (Illumina).
GEO Accession
GSM4649209