16S rRNA gene amplicon sequencing (V4 region): homo sapiens: adult female stool
DNA extraction: PCR amplification: PCR product purificication using solid-phase reversible immobilization beads: Illumina V2 500 cycle kit (2x 250/250) on MiSeq with 15% PhiX mock library: (All steps conducted as per Earth Microbiome Project standards/guidelines)