GSM2795974: SMAD2/3, BT549, TGFβ 16h_ChIP; Homo sapiens; ChIP-Seq
Chromatin isolation, sonication and immunoprecipitation using anti-SMAD2/3 antibody (#610843, BD Transduction Laboratories) were performed essentially as described (Isogaya et al., 2014; Morikawa et al., 2016). The libraries were constructed as described (Murai et al, 2015), using IonXpress Plus Fragment Library Kit (Thermo Fisher Scientific). Adaptor-ligated samples were amplified by 15 cycles of PCR and purified by E-Gel SizeSelect (Thermo Fisher Scientific).
GEO Accession
GSM2795974