GSM3893406: Subject 7 technical replicate 8; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNAeasy Mini Plus Kit (Qiagen) 10 ng of total RNA were amplified and converted to cDNA using SMART-Seq v4 Ultra Low Input RNA kit (Clontech). Afterwards an average of 150 pg of amplified cDNA was used to prepare library following Nextera XT DNA kit (Illumina)