RNA-Seq of Metallosphaera sedula DSM 5348 propagated with yeast extracts
mRNA was fragmented into small pieces using divalent cations under elevated temperature. The cleaved RNA fragments were then copied into first strand cDNA using random primers and reverse transcriptase. Second strand cDNA synthesis followed, using RNase H and DNA Polymerase. The cDNA fragments then went through an end repair process, the addition of a single A base to 3 ends, and then ligation of the adapters. The products are then purified and enriched with PCR to create the final cDNA library.