mRNA seq for the study of retinal regeneration
Total RNA was purified from target tissues conventionally and evaluated by Agilent 2100 Bioanalyzer  (Agilent Technology, Santa Clara CA 95051). From this qualified RNA sample, a cDNA library (insert  size: 300-400 bp) was constructed according to the instruction provided by Illumina (San Diego, CA  92122). Paired-end sequencing (100 bp read x 2) was carried out by Illumina HiSeq2500 and a set of  raw read data was produced after base calling and Chastity filtering (CASAVA ver.1.8.1., Illumina).