GSM4929989: 16h_IL2_8; Mus musculus; ATAC-seq
50,000 unfixed nuclei were tagged using Tn5 transposase  for 30 min at 37°C. Libraries were generated using the Ad1_noMX and Ad2.1-24 barcoded primers and amplified for 10-12 cycles. Resulting library fragments were purified using a DNA Clean & Concentrator-5 kit (Zymo Research). Size-selection was performed using the Agencourt AMPure XP PCR purification system (Beckman Coulter) to remove large fragments > 1 kb and primers to maintain high library complexity. Libraries qualities were assessed using the Agilent Bioanalyzer High-Sensitivity DNA kit and sequenced on an Illumina NextSeq500 with a read length of 75 bp to a minimum depth of 40 million reads per sample using the NextSeq 500 High-Output Kit (150-Cycle, 400 M Flow Cell). Two technical replicates were processed per biological sample.
GEO Accession
GSM4929989