RNA-seq of Pimephales promelas: Adult (6-8 months) pooled brain, liver, gonad, heart, kidney, and gill
Brain, liver, gonad, muscle, heart (n=3 from each sex), kidney, and gill isolated from 5 females and 5 males. Tissue snap frozen at -80 C until processed. RNA for each tissue-gender combination pooled (7 tissues x 2 genders). Equal amounts of RNA from each of 14 pools combined to make final pool for sequencing.