Fat body DNA from Diploptera punctata to recover Blattabacterium
Bacterial DNA was collected from fat body tissue dissected from female D. punctata using a modified version of previously described protocols (8, 9) with the use of a QIAGEN DNeasy Blood & Tissue kit. Samples were homogenized in 200 l of sterile 1X PBS. This extract was passed through a 20 m glass syringe filter (Millipore) and centrifuged for 10 minutes at 8000xg and 4C. The resulting pellet was resuspended in the extraction kit lysis buffer and DNA was extracted following manufacturer protocol. Illuminia sequencing was used to recover genomic sequences.