Illumina paired end reads from Raphanus raphanistrum
Raphanus raphanistrum (RR) is an obligate out-crosser. To reduce the amount of heterozygosity in the genome, RR subspecies raphanistrum (weedy) from the Binghampton population in New York was inbred for five generations. Total DNA was extracted from the leaves of the 5th generation inbred plants using Qiagen DNEasy Maxi kit. The extracted DNA ethanol precipitated and assessed for quality using CHEF gel electrophoresis. DNA was further sheared and an Illumina fragment library constructed. A total of 7 lanes of 100 bp paired-end sequence was generated on an Illumina GAII sequence analyzer.