GSM2872470: WAP+ cell 3; Mus musculus; RNA-Seq
Hundreds of cells for each sample were isolated by FACS using TVA antibody. Cell lysis, Reverse transcription and cDNA amplification was performed according to the manufacturer's instructions of SMART-Seq v4 Ultra Low Input RNA Kit (Clontech). Amplified cDNA was purified using Agencourt AMPure XP Kit (Beckman Coulter). The full-length cDNA output was processed with the Nextera Library Preparation Kit (Illumina) to prepare cDNA libraries for RNA-Seq experiments. All cDNA libraries were sequenced on a HiSeq 2500 (Illumina) platform. 100 bp paired end short reads were generated for subsequent Bioinformatics analysis.
GEO Accession
GSM2872470