GSM3580553: WT_Mu2; Mus musculus; RNA-Seq
Wild-type, IFNgR2-, and Jak1-mutant tumor cells were inoculated into C57BL/6 mice and tumors were extracted 7 days later. Tumors were enzymatically digested for 30 min at 37oC with rotation. Tumor suspension was Ficolled and buffer layer was washed twice with PBS. Cells were stained with CD45, CD3, Thy1, CD19 antibodies and a live-dead permeability dye to gate out hematopoietic cells and dead cells. Tumor cells were cell sorted based on dsRed and BFP expression into RLT buffer and quickly frozen.  RNA was extracted using the RNEasy micro kit (QIAGEN) and submitted for single-end RNA sequencing. RNA was extracted using the RNEasy Kit (QIAGEN) following manufacturer's proctocol.
GEO Accession
GSM3580553