Light induced nucleosome changes in Neurospora crassa with sub1 knock-out strain
Cultures were grown in Vogel`s medium with 2% glucose and grown in light for 2 days and transferred to constant darkness for 24h. They are cross-linked after 0, 20 and 120 min light exposure with 0.5 % formaldehyde for 10 min, quenched with 125 mM glycine and harvested.  After MNase digestion, purified DNA has been used to prepare library without a size selection to gather shorter/longer nucleosome fragments and possible transcription factor protected fingerprint DNA. NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (E7370L) was used for library preparation