GSM3350354: SD_ZT4_1; Medicago truncatula; RNA-Seq
In the first experiment, sampling occurred from SD and LD shifted plants when the plants were 13 days old at ZT0 and two hours later at ZT2 and in the second experiment sampling occurred at ZT4 when the plants were 15 days old.  Three biological replicates were taken each consisting of two pooled trifoliate leaves from different non-adjacent plants. Only the first trifoliate leaf to unfurl was sampled from a given plant.  Samples were immediately frozen in liquid nitrogen. Total RNA was extracted using RNeasy Plant Mini Kit (Qiagen, Germany) following the manufacturer's instructions. Library construction was performed by the Otago Genomics Facility using ScriptSeq Complete Kit (Plant) (Illumina Inc., USA) in the first experiment while the second experiment (ZT4 samples) used TruSeq Stranded mRNA Kits (Illumina Inc., USA).
GEO Accession
GSM3350354