Illumina HiSeq 2000 paired end sequencing; Concurrent DNA copy number alterations and mutations in genes related to maintenance of genome stability in uninvolved glandular tissue from breast cancer patients.
Concurrent DNA copy number alterations and mutations in genes related to maintenance of genome stability in uninvolved glandular tissue from breast cancer patients.
A custom SureSelect target enrichment library (design ID 0488011) was designed using the SureDesign online tool (Agilent Technologies, https://earray.chem.agilent.com/suredesign/). The library consisted of 42155 probes (2.6 Mbp) which encompassed 301 targets comprising 3059 genomic regions at 97.5% average coverage. The library targeted exonic as well as noncoding elements that were previously identified as predisposition loci, or were affected by somatic driver mutations in breast cancer.
Experimental Factor: genotype
Copy Number Alterations: 8
LIBRARY_STRAND
not applicable