GSM3351359: P0 Gjd3-CreEGFP mouse AVCS scRNA-seq library; Mus musculus; RNA-Seq
Single cell suspension was washed with 1X PBS (0.04% BSA) before 10x Genomics procedure. The concentration of single cell suspension was adjusted to about 500 to 1000 cells/µL and was loaded on the 10x Genomics' Chromium™ system (10x Genomics, Pleasanton, CA) with the aim of 6000 to 10000 cells per channel (Chromium™ Single Cell 3' Library & Gel Bead Kit v2, catalog number 120237). Single cell RNA sequencing libraries were constructed following the manufacturer's instructions (Zheng et al., 2017).
GEO Accession
GSM3351359