Renal biopsy single-cell RNA-sequencing: Sample COL20_ROW20_METRO0352.Skin
Samples were obtained through skin punch biopsies or renal biopsies from SLE and healthy patients. Tissues were disaggregated using Liberase, loaded onto medium 10-17 µm diameter C1 HT 800-well integrated microfluidic chips (IFC) (Fluidigm) and processed according to Fluidigm C1 HT protocol. Libraries created using the Nextera XT Library Preparation Kit (Illumina) with indexing according to the Fluidigm C1 HT protocol revision A.