GSM3660742: ChIP-seq.NELFA_plusDox_rep3_HAantibody1; Mus musculus; ChIP-Seq
ChIP-seq: Dox-induced were fixed with formaldehyde (final 1%) for 10 minutes. Fixed cells were then subjected to standard ChIP lysis protocol to release chromatin. Released Chromatin was sonicated for 15 cycles (30 sec on, 30 sec off) on a Biorupter at high setting. Soluble chromatin was collected and between 1-2 mg was used for IP with 4-8 ug of respective Dyna-protein G conjugated antibody complex. Captured fragments were subjected to several rounds of stringent salt washes before eluting with SDS buffer. ChIP-seq: Eluted DNA was purified and subjected to NGS library preparation using the NEBNext DNA ultra II kit. Constructed ChIP libraries were sequenced on the Illumina NextSeq or HiSeq platform.
GEO Accession
GSM3660742