GSM2527059: Naïve stem cells_7 (N7); Mus musculus; RNA-Seq
cDNA was directly synthesized from cell lysate and amplified by PCR, using SMARTer Ultra Low Input RNA kit for sequencing-v3 (Clontech Laboratories, Mountain View, CA). The Illumina Nextera XT DNA sample Prep Kit (Illumina, San Diego, CA) was used to generate sequencing libraries from the cDNA.  Sequencing libraries were validated, using the Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA), and quantified, using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA) as well as real-time PCR (Applied Biosystems, Carlsbad, CA).  The sequencing libraries were sequenced on the Illumina HiSeq 2500 instrument, according to manufacturer's instruction.
GEO Accession
GSM2527059