Identification of WCC targets by MNase-ChIPseq
ChIP of wt strain was performed to 24h dark grown, 1min and 30min light induced cultures by using WC2 antibody. Gentle fragmantention was performed by using MNase digestion. ChIP DNA libraries were prepared with NEBNext® ChIP-Seq Library Prep Reagent Set for Illumina® with NEBNext® Multiplex oligos