GSM1194142: input DNA; Mus musculus; ChIP-Seq
Liver tissue was fixed with formaldehyde, lysed, sonicated, and GATA4-DNA complexes were isolated with antibody.  DNA was purified using phenol/chloroform extraction and ethanol precipitation. Libraries were prepared with Illumina ChIP-Seq DNA Sample Prep kit Cat # IP-102-1001.  The input (liver) and ChIP DNA fragments were repaired to generate blunt ends, with a single adenine added to each end. Illumina genomic adaptors were ligated to both ends of the fragments, ligation products were amplified by 18 cycles of PCR, and the PCR products between 250 and 450 bp were gel purified according to standard Illumina protocols. The quantity and quality of each library was evaluated by qPCR and Bioanalyzer (Agilent Technologies, Santa Clara, CA). Cluster generation and sequencing chemistry were performed using Illumina-supplied kits as appropriate. The ChIP DNA library was sequenced in single-read mode on the Illumina HiSeq 2000 platform.
GEO Accession
GSM1194142