GSM3579104: Trpv1 replicate 2; Mus musculus; RNA-Seq
With 3-5 weeks of age, DRG neurons were acutely dissociated from mice of both genders as described previously (Li et al., Proc Natl Acad Sci U S A, 2017). Briefly, DRG were harvested and incubated in 5 mg/mL Collagenase/Dispase (10269638001, Millipore-Sigma) for 30 minutes. Cells were mechanically dissociated and between 150-250 eGFP- or tdTomato-positive cells per mouse were hand-picked. Each picking session included one sample per genotype to potentially identify experimentally induced biases. A total of 4 independent samples per genotype were generated. Total RNA was extracted with a PicoPure™ RNA isolation kit (ThermoFisher Scientific, Waltham, MA) using the manufacturer's instructions. cDNA was produced and amplified using the Ovation® RNA-seq system V2 (NuGEN, San Carlos, CA) according the manufacturer's protocol. Sequencing libraries were generated using the Nextera XT method (Illumina, San Diego, CA).
GEO Accession
GSM3579104