Complete genome sequence of Paracoccus mutanolyticus RSP-02 isolated from cellulosic waste
Whole genome libraries were constructed by following manufacturers protocol (NEBNext Ultra II DNA Library Prep Kit for Illumina). For each sample, 500 ng genomic DNA was randomly fragmented to <300 bp by sonication (Covaris M220). The fragments were treated with End Prep Enzyme Mix for end repairing, then immediately adaptors were ligated to both ends. After purification size selection using Agencourt AMPure XP beads - Beckman Coulter, the results were amplified and barcoded by using PCR primers and indices unique. The PCR products were again purified Agencourt AMPure XP beads and the purified products were  validated by using an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA), and did Qubit3.0 to detect size  and concentration of libraries, respectively.  According to manufacturers instructions (Illumina, San Diego, CA, USA), the libraries with different indices were mixed and loaded on Illumina NextSeq 500 for 2x150 paired-end (PE) sequencing. Primary analysis was performed by built-in software, NextSeeq Control Software (NCS), and demultiplexing was performed by bcl2fastq 2.17.