Transcriptomic analysis of Exiguobacterium antarcticum B7 during cold adaptation
After RNA extraction, messenger RNA was enriched using Ribominus Transcriptome Isolation Kit, and the amount of recovered RNA was measured. The enriched RNA samples were used to build strand-specific RNA-Seq libraries, that were prepared using a SOLiD™ Total RNA-Seq Kit. Fifty-base pair (bp) fragments were sequenced using the SOLiD 3 Plus system according to the manufacturer’s instructions.