genomic reads for T. brucei with ORF lib untreated
genomic DNA was isolated from 100-200 million transfected parasites and fragmented by sonicating water bath (Bioruptor). The NEBNext Ultra II DNA library prep kit was then used to prepare libraries for high throughput sequencing according to the manufacturers instructions with the following exception: a custom universal primer containing the ATT cloning site was used for 5 indexing and PCR enrichment (5AATGATACGGCGACCACCGAGATATATAACAAGTTTGTACA AAAAAGCAGGCTATG). A custom sequencing oligo (5-GGGACAAGTTTGTAC AAAAAAGCAGGCTATG) is loaded on to the Illumina sequencing platform to sequence only GoF library containing reads.