GSM1836379: Lab A 15C; Caenorhabditis elegans; RNA-Seq
Messenger RNA was isolated from C. elegans cultured at 15ºC and 25ºC using an RNeasy Mini kit (Qiagen, Valencia, CA) or Purelink (Ambion), and treated with DNase I (Qiagen) following manufacturer’s instructions. Integrity of RNA was determined using the Experion™ automated electrophoresis system (Bio-Rad, Hercules, CA). RNA with an RNA integrity number >8.9 underwent further analysis. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM1836379