GSM1348956: MCF7_WT_1; Homo sapiens; RNA-Seq
Total RNA extractions were performed using miRNeasy kit (Qiagen) as recommended by the manufacturer. RNA integrity of each sample was assessed with an Agilent 2100 Bioanalyzer. Small RNA (<200 nucleotides in length) were isolated using a low molecular weight RNA extraction kit (mirVana, Invitrogen). cDNA libraries were prepared using the NEBNext Multiplex Small RNA Library Preparation Set for Illumina. The resulting cDNA libraries were paired-end sequenced on Illumina HiSeq sequencers at the McGill University and Genome Quebec Innovation Centre. For these sequencing runs, 8 samples were multiplexed per lane, resulting in between 15M and 24M paired reads per dataset. Paired-end sequencing was chosen because the length of some of the molecules of interest surpassed the read length (which was 100 nt). Additionally, the paired-end sequencing ensured high-quality sequences at both ends of the RNAs of interest.
GEO Accession
GSM1348956