GSM4119003: RNA-seq, Primary CD4+ T-cells, 4hr stimulated, replicate 1; Homo sapiens; RNA-Seq
Five million CD4+ T-cells were harvested, stored in Qiagen RNAprotect solution and the nuclear RNA isolated using a Qiagen RNeasy kit and quantified. Samples were either pooled in equal amounts (same individuals as for Hi-C to create matched samples), or processed individually to give duplicate samples. Libraries for RNA-seq were prepared using the NEB Next Ultra Directional RNA-seq reagents and protocol using 100ng of nuclear RNA as Input. Primary human CD4+ T-cells were isolated from PBMCs using an EasySep T-cell isolation kit (StemCell)
GEO Accession
GSM4119003