GSM2967469: IsletBeta1; Homo sapiens; RNA-Seq
Total RNA was isolated from GFP-high cells FACS sorted from d27 eBCs, NECs and d20 immature clusters; and β-cells sorted from adult human islets using RNeasy Micro kits (Qiagen). RNA isolated from sorted cells was further concentrated employing RNA Clean & Concentrator (Zymo research). rRNA was depleted by RiboGone (Clontech), and 325-350bp sized strand-specific cDNA libraries were prepared using the SMARTer Stranded Total RNA Sample Prep Kit – Low Input Mammalian Kit (Clontech).
GEO Accession
GSM2967469