GSM3783518: IgG ChIP-Seq; Homo sapiens; ChIP-Seq
30 million K562 cells per sample were cross-linked for 10 minutes at room temperature with 1% formaldehyde. Nuclei were isolated and sonicated in lysis buffer (20 mM Tris-HCl pH 8.0, 150 mM NaCl, 2 mM EDTA, 0.1% SDS, 1% Triton X-100) on a Diagenode Standard Bioruptor for 18 cycles at 20 seconds each with 60 seconds rest between cycles. Sonicated lysates were then immunoprecipitated by overnight incubation at 4°C with Dynabeads magnetic protein G beads (Thermofisher) bound with antibody against protein of choice. ChIP samples were converted into libraries by the Yale Center for Genome Analysis facility using the Illumina ChIP sequencing library preparation kit and sequenced using the high-throughput Illumina platform.
GEO Accession
GSM3783518