RNA-Seq of 450-470 micron follicles: gravid female  Epinephelus coioides
mRNA was enriched by Oligo(dT) beads, fragmented into short fragments and reverse transcripted into cDNA with random primers. The cDNA fragments were purified, end repaired, poly(A) added, and ligated to Illumina sequencing adapters. The ligation products were size selected, PCR amplified, and sequenced using Illumina HiSeqTM 2500.