RNA-Seq of E. californica carpels
RNA samples were pre-amplified using Nugen Ovation RNASeq System suitable for an input of 500 pg - 100 ng of RNA and the cDNA generated was used for library preparation. Amplification and cDNA generation was conducted according to the manufacturers instructions.Library preparation was conducted with the Illumina Nextera XT kit suitable for 1ng DNA. The cDNA was measured before the preparation with Qubit and normalized to 0,2ng/l. 5l of dilution was used for the library preparation. Libraries were prepared according to the manufacturers instructions. Generated libraries were sequenced with Sequencing HiSeq 4000. 9 samples were sequenced per lane generating approximately 33Mio reads per sample. Paired-end sequencing was used for each sample with read length of approximately 76 bp.