454 mate paired reads from Raphanus raphanistrum
Raphanus raphanistrum (RR) is an obligate out-crosser. To reduce the amount of heterozygosity in the genome, RR subspecies raphanistrum (weedy) from the Binghampton population in New York was inbred for five generations. Total DNA was extracted from the leaves of the 5th generation inbred plants using Qiagen DNEasy Maxi kit. The extracted DNA ethanol precipitated and assessed for quality using CHEF gel electrophoresis.  For 454 sequencing, DNA was sheared by Covaris sonication, size selected by gel electrophoresis and a 3 kb mate pair library constructed according to manufacturer’s instructions (Roche-454). A total of 6 full plates and three half-plates were sequenced using the Titanium chemistry.