RNAseq of E. coli EHEC-ESBL 22207 growth with no ciprofloxacin
Each library was uniquely tagged with one of the Illumina’s ScriptSeqTMv2 library barcodes. Library quantification was performed using the DNA1000 kit (Agilent). Each Library concentration was normalized at 4nM by qPCR and pooled at 2nM. Libraries were denatured using the Denaturing and Diluting Libraries for the HiSeq® and GAIIx protocol.