GSM4212800: RNASeq of mock treated RAW264.7 cells 2; Mus musculus; RNA-Seq
DualSeq experiment: Medium was discarded. Cells were scratched off and resuspend in RNAprotect. RNA extraction was done as previously described (Kordes et al., 2018). Spermidine experiment: Medium was discarded. Cells were scratched off and resuspend in RNA buffer. RNA extraction was done as previously described (Kordes et al., 2018). DualSeq experiment: NEBNext Ultra II Directional RNA Library Prep. Spermidine experiment: NEBNext® Single Cell/Low Input RNA Kit.
GEO Accession
GSM4212800