Illumina HiSeq 2000 paired end sequencing; The ChIP-Seq binding peak data of the H4K20me3 in mESCs, Pwp1(-/+) ESCs and Pwp1-overexpressed Pwp1(-/+) ESCs
The ChIP-Seq binding peak data of the H4K20me3 in mESCs, Pwp1(-/+) ESCs and Pwp1-overexpressed Pwp1(-/+) ESCs
mES cells (E14.1) were cultured on plates coated with 0.1% gelatin (Millipore) in high-glucose Dulbecco's modiﬁed Eagle's medium (DMEM; HyClone) supplemented with 15% ES cell-qualiﬁed fetal bovine serum (Gibco), 1% penicillin and streptomycin (Gibco), 2 mM L-glutamine (Gibco), 0.1 mM non-essential amino acids (NEAAs; Gibco), 0.1 mM 2-mercaptoethanol (Gibco) and 1000 units/ml of LIF (Chemicon). Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. Libraries were prepared for sequencing using standard Illumina protocols at BGI-Shenzhen (sequencing service provider).
Experimental Factor: genotype
Pwp1 -/+ and Pwp1 overexpression
Experimental Factor: immunoprecipitate
H4K20me3