GSM1356023: Rex rabbit, back skin, 42 days, black color(B); Oryctolagus cuniculus; RNA-Seq
Total RNA was extracted from the back skin of the Rex rabbits using TRIzol reagent (Invitrogen, USA). A Qubit fluorometer (Invitrogen, USA) and an Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., USA) were used to determine the quality and quantity of the RNA. The mRNA was enriched using oligo (dT) magnetic beads, then fragmented into short fragments (approximately 200-700 nt) using fragmentation buffer (Invitrogen, USA). Double-stranded cDNA product was synthetized and purified . RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM1356023