GSM1187623: ChIP_WT_H3K4me3_8wks_ctx_rep1_rep2; Mus musculus; ChIP-Seq
Cortical and striatal tissues were crosslinked with 1% formaldehyde for ten minutes and the crosslinking was quenched by 0.125M final concentration of glycine. The crosslinked tissue was then homogenized, rinsed with PBS, pelleted, and frozen in liquid nitrogen for later use. Lysates were clarified from sonicated nuclei, and protein-DNA complexes were isolated with antibody (H3K4me3; Millipore, cat 17-614). Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303).
GEO Accession
GSM1187623