GSM4257000: Gilteritinib_2; Homo sapiens; OTHER
Cells were harvested, DNA extracted via Qiagen Blood & Cell Culture DNA Midi Kit per manufacturer's instructions, and the sgRNA cassette retrieved via PCR as described by Broad sequencing protocol on Addgene. The P5 mix and P7 primers design from this protocol were used with the following New England Biolabs NEBNext Indexes: 17, 24, 26, 28, 29, 31, 33, 34, 35, 36, 42.
GEO Accession
GSM4257000