Nanopore sequencing of Streptococcus pyogenes SP1336
1.5 ug of genomic DNA was used as input for the Oxford Nanopore 1D sequencing by ligation kit (SQK-LSK108), as per manufacturer?s instructions. The final library was sequenced for approximately 46- hours on an R9.4 SpotON flow cell using the MinION, MinKnow software version 2.0 with local basecalling performed using Albacore version 2.1.3.