GSM1187198: Human_MeDIP; Homo sapiens; MeDIP-Seq
MagMeDIP kit (Diagenode) for Immunoprecipitation Pooled DNA from these samples was subjected to AutoMeDIP-seq as previously described (Butcher et al. Methods (2010) 52, 223-31), using the NEBNext DNA Sample Prep Mastermix (New England Biolabs) for the library preparation and the MagMeDIP kit (Diagenode) for the immunoprecipitation. Adequate enrichment of the methylated DNA fraction (compared to input) was quality controlled using qPCR. Following adapter-mediated PCR, the library was subjected to size selection (300-350 bp) from low melting-point agarose gels. The excised fraction was quality controlled by qPCR. Cluster generation and 36 base paired-end sequencing was performed by the UCL Genomics Core Facility according to the manufacturer’s recommendation, using an Illumina GAIIX platform.
GEO Accession
GSM1187198