16s V4 MRSA-CPS matched sites
Twenty-five households containing 56 pets and 30 humans were studied. Sampling locations were matched to anatomical sites cultured for MRSA (people) or CPS (pets). Microbiota were characterized using culture-independent sequencing of the bacterial 16S ribosomal RNA (rRNA) gene.
16S amplicons were generated following procedures outlined by Caporaso et al. (2012). Caporaso JG, et al. 2012. Ultra-high throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms. ISME J. 6(8): 1621-1624.