16S amplicon sequencing of terrestrial leaf metagenome: FC G 21-Aug-2016
The V4 hypervariable region of the 16S rRNA gene was amplified using dual indexed Illumina compatible primers 515f/806r as described by Kozich, JJ (2013). PCR products were normalized using Invitrogen SequalPrep DNA Normalization plates and the products recovered from the plates pooled. This pool was cleaned up with AMPureXP magnetic SPRI beads. The pool was QCd and quantified using a combination of Qubit dsDNA HS, Caliper LabChipGX HS DNA and Kapa Illumina Library Quantification qPCR assays.