GSM2192639: AEBP2_204_wild_type_rep1_ChIPSeq; Mus musculus; ChIP-Seq
ChIP for FS2-tagged AEBP2 was performed as described (Farcas et al., 2012) except that we used rProtein A Sepharose beads (GE Healthcare) that had been blocked for 1 hr at 4 °C with 1 mg/ml Bovine Serum Albumin (BSA) and 1 mg/ml yeast tRNA (Sigma). ChIP for endogenous proteins was performed as described (Stock et al., 2007), except approximate concentration after sonication was measured by measuring absorbance using the Nanodrop ND-1000 (ThermoScientific) and 200 μg of chromatin was used per IP and after the washes DNA was purified using ChIP DNA Clean & concentration™ columns (Zymo 13 Research) and eluted in 10 μl volume. NEB Next® DNA Library Prep Master Mix Set for Illumina
GEO Accession
GSM2192639