metagenome of bagasse fosmid library
Metagenomic fosmid library from bagasse sample was constructed using CopyControl™ fosmid library production kit (Epicentre Biotechnologies, Madison, WI) according to the manufacturer’s instruction, with slight modifications. The purified DNA was end-repaired to generate blunt 5’-phospholyrate ends and then ligated to pCC1FOS vector at 25C for 3 h. The ligated DNA was packaged using the lambda packaging extract supplied and subsequently transformed into E. coli EPI300-T1R. The transformants were selected on LB agar plates supplemented with 12.5 µg/ml of chloramphenicol. The library was stored at -80 °C in 15% glycerol as pool libraries