GSM1480601: pAminus RNA-seq from mouse ESC R1; Mus musculus; RNA-Seq
Total RNAs were prepared using Trizol Reagent and treatment with DNase I. 20μg of total RNAs were separated into polyadenylated and nonpolyadenylated fraction by oligo(dT) magnetic beads (Invitrogen). Ribosomal RNAs were further depleted in nonpolyadenylated fractionby using RiboMinus kit (Invitrogen) to get the poly(A)-/ribo- RNA population. Library was prepared by using Illumina TruSeq RNA Sample Prep Kit V2.
GEO Accession
GSM1480601