Transcriptome of Andes-virus Seropositive Oligoryzomys longicaudatus spleens-29-Spleen-seropos-viruspos
Rice rats were collected in the field of Peru and tested for antibodies to Andes virus.  Five spleens were collected for further analysis, two from seronegative and three from seropositive animals.  Total RNA was isolated from spleens using Trizol reagent (Life Technologies).  RNA-seq libraries were prepared from 500ng total RNA using Ribo-Zero (Illumina) library preparation methods and the manufacturer’s recommended procedure.  5 spleen RNA-Seq libraries were prepared separately and pooled on a single HiSeq 2000 (Illumina) lane for paired end 2x100nt sequencing.  Library preparation and sequencing were performed at the CU-Anschutz core facility.