GSM2526979: mock_11; Drosophila melanogaster; OTHER
neuRNA labeling and capture were performed with Click-iT Nascent RNA Capture Kit (Thermo Fisher Scientific) according to manufacturer’s protocol with the following minor modifications. Cells were incubated with EU at  0.2 mM for 1 h and RNA was extracted with TRizol (Thermo Fisher Scientific). The Click-iT reaction was performed with 0.25 mM biotin azide, and biotinylated RNA was captured with 12 μL T1 beads. Nascent RNA was used to generate RNA-seq libraries with Ovation RNA-seq Systems 1-16 for Model Organisms (Nugen). All samples were sequenced with HiSeq2500 (Illumina) at the NIDDK Genomics Core Facility by 50 bp single-end sequencing. neuRNA-seq
GEO Accession
GSM2526979