APMV-8 random PCR with primer FR20RV-6N, repeat 1
An avian paramyxovirus type 8 (APMV-8) isolate was centrifuged, filtrated and nuclease treated to enrich for virions. After viral RNA extraction, sequence independent primer amplification (SISPA) was performed with random SISPA primer FR20RV-6N. This primer is composed of a random 6N oligomer tagged with a known sequence.  Purified and size selected (400-1200nt) random PCR amplicons were used as input for the sequencing library.