GSM1843384: 100pg 600nM rep1; Homo sapiens; ChIP-Seq
Chromatin immunoprecipitation (ChIP) assay was performed as previously described (Scharer et al. Cancer Research, 69:709-717).  Briefly, 10^7 cells were fixed in 1% formaldehyde for 10 minutes, nuclei isolated, and sonicated to an average chromatin fragment size of 200-600 bp.  30 mg chromatin was immunoprecipitated with 3 μg anti-H3K4me3 antibody over night at 4 degrees.  Antibody-chromatin complexes were captured with Protein A magnetic beads (Invitrogen), cross-links reversed, and DNA purified.  ChIP DNA was quantitated by Qubit assay and diluted to the indicated starting amounts. ChIP-seq libraries were generated using the indicated amount of input DNA with the KAPA Hyper Prep Kit according to the manufacturers instructions.
GEO Accession
GSM1843384