GSM1482481: D14-2358_Control; Homo sapiens; RNA-Seq
Total RNA was isolated using the RNeasy Mini Kit (QIAGEN).  The purified RNA was the subjected to oligo (dT) selection, fragmentation and first and double strand synthesis with the Illumina Tru-Seq kit using manufacturer’s instructions. DNA fragments were purified using SPRI-TE beads according to manufacturer’s instructions. The purified DNA was end repaired and single A bases were added for adaptor ligations. The adaptor-ligated DNA was then subjected to double SPRI purification to select for DNA inserts between 150 and 350nt. These fragments were enriched and barcoded by PCR for multiplexing. A final SPRI purification at 1.5X was performed to clean up the barcoded RNA-Seq libraries. Libraries were quantified by Advanced Analytical Fragment Analyzer and by qPCR and sequenced on a HiSeq2000 for 50nt"
GEO Accession
GSM1482481