GSM3350366: P11-1; Aspergillus niger; RNA-Seq
The mycelia growing on each of the compartments was sampled by cutting through the polycarbonate (PC) membrane along the boundary of the compartment with a scalpel blade and then the PC membrane was peeled away before transferring the mycelia to a 2 mL tube and flash freezing in liquid nitrogen; these samples were used for RNA isolation as described previously (PMID:26314379). The SMARTerR UltraTM Low RNA kit (catalog number 634851) was used to generate full length cDNA template library according to the instruction manual, followed by Ion Plus fragment library kit (Thermo Fisher Scientific, cat# 4471252) to fragment cDNA and barcode adapter ligation for template prep.  Template library was quantified using the Ion Library TaqMan™ Quantitation Kit (Thermo Fisher Scientific, cat# 4468802). The emulsion clonal bead amplification to generate bead templates for Ion Torrent platform using the Ion PI™ Hi-Q™ Chef Kit (Thermo Fisher Scientific, A27198) with Ion PI™ Chip Kit v3 (Thermo Fisher Scientific, cat#A26771) on the Ion Chef System (Thermo Fisher Scientific). Sequencing was performed using the Ion PI™ Hi-Q™ Sequencing 200 Kit (Thermo Fisher Scientific, cat# A26772) on Ion Proton sequencer.
GEO Accession
GSM3350366