Plant C - Control without  wastewater - day 3
The PCR was carried out in two-steps; first amplifying the V3-V4 region using the primer pair PRK341F(5-CCTACGGGRBGCASCAG-3)/PRK806R(5-GGACTACYVGGGTATCTAAT-3) (Yu et al., 2005), followed by a second PCR for attachment of dual indices and Illumina sequencing adaptors. The lib was purified  (AMPure XP beads) and normalized , and  sequenced using the MiSeq v3 reagent kit with 300 PE reads.