Acidovorax citrulli M6 PacBio subreads
Acidovorax citrulli M6 was isolated in Israel in 2002 from a symptomatic melon fruit (Burdman et al., 2005) and grown on nutrient broth (NB, Difco Laboratories, Detroit, MI, USA) or NA (NB containing 15 g/l agar) at 28C. Genomic DNA was isolated with the GenEluteTM Bacterial Genomic DNA Kit (Sigma-Aldrich, St. Louis, MO, USA) according to the manufacturers instructions. The DNA was sent to Macrogen (Seoul, South Korea) for sequencing. The sample was prepared according to standard instructions for SMRTbellTM templates for sequencing on the PacBio RS System, and sequenced using SMRT Sequencing.