GSM4119027: Hi-C, Primary CD4+ T-cells, 1hr stimulated, replicate 2; Homo sapiens; Hi-C
Cells were thawed on ice and re-suspended in 50ml ice-cold lysis buffer (10mM Tris-HCl pH 8, 10mM NaCl, 0.2% Igepal CA-630, one protease inhibitor cocktail tablet). Cells were lysed on ice for a total of 30 min, with 2x10 strokes of a dounce homogeniser with a 5 minute break between douncing. The nuclei were pelleted and permeabilised and the chromatin was digested with HindIII. Ends were marked with biotin followed by in-nucleus ligation, decrosslinking and biotin pulldown according to the previously established Hi-C protocol (Dryden et al., 2014; P. Martin et al., 2015). Primary human CD4+ T-cells were isolated from PBMCs using an EasySep T-cell isolation kit (StemCell)
GEO Accession
GSM4119027