GSM2193049: ME7_20150325_IDX6; Homo sapiens; RNA-Seq
For extraction, cells were lysed using RLT buffer then the lysate was homogenized using Qiashredder spin columns (Qiagen, Hilden, Germany).  RNA was isolated using RNeasy mini columns (Qiagen, Hilden, Germany) and quantitated using a Nanodrop (Thermo Fisher, Wilmington, DE), followed by Turbo DNase treatment (Thermo Fisher, Wilmington, DE) at 37°C for 30 minutes and re-quantification. After repairing the RNA ends, sequencing adaptors (Illumina, San Diego, CA) were ligated to the purified RNA, and the RNA was amplified for sequencing.
GEO Accession
GSM2193049