GSM3351825: CD4+Tcells_CpGA-pDC_I; Homo sapiens; RNA-Seq
Total RNA was isolated from cell lysates using a modified protocol for the RNAqueous™ Micro Total RNA Isolation Kit (Thermo Fisher) including on-column DNase digestion and analyzed for quality using the RNA 6000 Pico Kit (Agilent) Poly-A enriched NGS library construction was performed using the KAPA mRNA Hyper Prep Kit (KAPA Biosystems) using 500ng of input total RNA according to manufacturer's protocol using 9 amplification cycles. Individual libraries were quantitated via qPCR using the KAPA Library Quantification Kit, Universal (KAPA Biosystems) and equimolar pooled.
GEO Accession
GSM3351825