GSM1654362: Gcn5_WT_1_RNASEQ; Mus musculus; RNA-Seq
Total cellular RNA and genomic DNA were isolated from 1.0 x 106 cells of the 4-1, 4C2, 6-1 and 6C4 lines using the AllPrep DNA/RNA Mini Kit (Qiagen). The concentration of genomic DNA was monitored to ensure that the samples were nearly equivalent (within 7.5 % of each other). 1 μg of RNA was subsequently spiked with 2 μL of 1:100 ERCC Spike-In Mix #1 or #2 prior to RNA-Seq library preparation. RNASeq Libraries were prepared according to the Illumina TruSeq Strandard Total RNA Sample Preparation Kit (with Ribo-Zero Gold, RS-122-2301), 10 pM of each library was sequenced according to standard Illumina protocols on an Illumina HiSeq 2000.
GEO Accession
GSM1654362