Mayo Clinic GBM PDX
Whole-exome sequencing (WES) was performed using DNA isolated from a single flash frozen flank tumor specimen for each PDX model. Paired-end libraries were prepared by using the manufacturer's protocol, and exome capture was carried out with SureSelect Human All Exon V5+UTRs (or V4+UTRs) kit from Agilent (Santa Clara, CA), or TGen's Strexome V2 WES platform. The libraries were sequenced as 2X101 (or150) paired end reads on the Illumina HiSeq platforms according to the manufacturer's recommendation (Illumina Inc., San Diego, CA).