MiSeq WGS of Theileria orientalis: Ikeda
Piroplasm pellets were resuspended in 200 ľL of PBS and DNA was extracted using the DNeasy Blood and Tissue kit (Qiagen) according to the manufacturers instructions. For each sample, tagmentation of genomic DNA, and PCR amplification of tagged DNA were performed in triplicate using the Nextera system (Illumina). Sequencing libraries were pooled and normalized using bead size selection (SPRI beads, Beckman Coulter). Agilent 2100 Bioanalyzer, with High Sensitivity DNA kit was used to quantitate the pooled library before loading onto the Illumina platform.