HP015_HA_AX4
The HA and NA genes were amplified using RT-PCR and segment-specific primers. Multi-segment PCR amplicons were cleaned, and the concentration of purified amplicions was measured determined. After samples were normalized to a concentration of 0.2 ng/ul, adapters added by tagmentation. Samples were purified and fragment size distributions were analyzed on a Bioanalyzer. After bead-based normalization, sequence-ready libraries were sequenced in a paired-end run using the MiSeq.