Assai Fruit transcriptome
Sixteen fruits from different stages were randomly selected from four palms and used to prepare a cDNA library representative of fruit maturation. The ripening stages were based on fruits coloration according to Rogez et al., 2011: green fruits (94 days after full bloom); slightly mature fruits (124 days after full bloom), black fruits (150 days after full bloom) and fruits black covered with wax cuticle (162 days after full bloom). The four palms are located in Eastern Amazon region, in the estuary of Para river, in natural conditions of mangrove.  About 100 mg of each assai fruit exocarp and mesocarp were carefully ground with liquid nitrogen aid and total RNAs were extracted using RNeasy® Plant Mini Kit (Qiagen). Quality and quantity of total RNAs were determined by Nanodrop Lite Spectrophotometer (Thermo scientific, USA) and TapeStation 2200 (Aligent Technologies, USA). Only samples with high RNA Integrity Numbers values (RIN ≥ 7.0). The mRNA-seq library was constructed following the Ion Total RNA-seq Kit for the AB Library Builder System (Thermo Fischer, USA). Briefly, the mRNA was isolated and purified from total RNA samples using Dynabeads® mRNA DIRECT™ Micro Purification Kit (Thermo Fischer, USA) and then break to into small fragments by enzymatic fragmentation (RNase III). After the fragmentation, fragments purification was performed with Magnetic Bead Cleanup Module (Included in the Ion Total RNA-Seq kit) and then used as templates for cDNA library construction following to the AB Library Builder System. Each cDNA sample was amplified with add of PCR primers from Ion Express barcodes RNA-Seq Kit (Thermo Fischer, USA) and finally, 50 pmol of each library was pooled and sequenced on the Ion Proton™ Platform.