Extensive gene amplification as a mechanism for piperacillin/tazobactam resistance in a clinical isolate of Escherichia coli
E. coli 907355 genomic DNA was isolated from cultures containing 0, 4/4 mg/ml, or 8/8 mg/ml TZP using the DNeasy Blood and Tissue Kit (Qiagen) and the MinION sequencing library was prepared from unsheared genomic DNA using the SQK-MAP005 2D library preparation kit (Oxford Nanopore Technologies, Oxford, UK); sequencing was performed using the R7.3 chemistry.