RNA-seq of the replete venom gland of U. yaschenkoi scorpion
The cDNA library for the high-throughput sequencing was made with Illumina ® TruSeq™ RNA Sample Preparation Kit. The cDNA library preparation consisted on the following steps: i) mRNA enrichment and fragmentation, ii) cDNA synthesis, iii) paired- end and adaptors ligation (adenylate 3’Ends), iv) PCR amplification (DNA enrichment) and v) High-throughput sequencing (RNA-Seq method, Illumina Next-Gen sequencing technology).