Artemisia tridentata subsp. wyomingensis for Insights into Three Transcriptomes of Big and Low Sagebrush
RNA Sequencing   Five half-sib seedlings from each A. tridentata ssp. wyomingensis (UTW1, 38.3279 N,   109.4352 W) A. tridentata ssp. tridentata (UTT2, 38.3060 N, 109.3876 W) and A. arbuscula  (CAV-1, 40.5049 N, 120.5617 W) were grown in a petri dish on top of wetted filter paper for 2   days. Seedlings were then flash frozen in liquid nitrogen and ground using a mortar and pestle.   RNA was extracted using a Norgen RNA Purification Kit (Norg1en Biotek Corp., Ontario,   Canada). Sequencing libraries were prepared using an Illumina Tru-seq RNA Kit V2. Libraries were then pooled and multiplexed on an Illumin MiSeq lane and sequenced as 250 bp paired-end reads.     Transcriptome Assembly   Illumina reads were trimmed for quality using default settings in the program Sickle   (github.com/najoshi/sickle). Reads were then assembled using the program SOAPdenovo-trans   (Luo et al., 2012) at variable k-mer lengths ranging from 35 to 127 in increments of 4. The best   assembly for each read set was based on N50 and the number of scaffolds. Other modified   parameters included the number of scaffolds > 800 base pairs (bp) and the number of bp in   scaffolds > 800 bp.