Metavirome of Idotea (Pentidotea) wosnesenskii
Isopods were rinsed with 0.02_m-filtered phosphate buffered saline (PBS) and homogenized in 2.0mm Bead Beater tubes for 10min prior to viral purification. Homogenates were pooled, 0.2_m syringe Þltered to reduce cellular contamination, and concentrated via polyethylene glycol (PEG) precipitation. Resuspended concentrates were enzymatically digested with nucleases to exclude non-encapsidated nucleic acids prior to DNA extraction using the ZR viral extraction kit. Extractions were enriched for circular ssDNA templates using isothermal rolling circle amplification, confirmed by PicoGreen incorporation and gel electrophoresis.  DNA was then fragmented and barcoded via the Nextera XT DNA Library Preparation Kit prior to 2x250bp paired-end Illumina MiSeq sequencing