WGS of c.526insTT mouse
Genomic DNA of each sample was randomly sheared into short fragments of about 350 bp, respectively. The obtained fragments were subjected to library construction using the NEBNext DNA Library Prep Kit, according to the manufacturer. Fragments (in 300-500 bp size) were PCR enriched by P5 and indexed P7 oligos. After purification and subsequent quality check, the resulted library sequencing was performed on Illumina sequencing platform, with the read length of PE150 bp at each end.