GSM4255968: mESCs, BR2_KO_15min_lab; Mus musculus; RNA-Seq
RNA was extracted using Trizol, according to manufacturer instructions. RNA was DNAse treated using RNeasy on column digestion according to manufacturer's instructions. Total RNA libraries were prepared from 10 ng of DNase-treated preexisting and newly transcribed RNA using Ovation® RNA-Seq. reads were first mapped to mouse ribosomal RNA sequences with STAR v2.5.0. Reads that do not map to ribosomal RNA were then aligned to intronic and exonic sequences using STAR and quantified using RSEM.
GEO Accession
GSM4255968