Chromatin accessibility, Danio rerio adult retinal ganglion  cells, 4 days post optic nerve crush, replicate 3
Chromatin was  extracted from nuclei isolated from FACS sorted RGCs, enzymatically  dissociated from adult zebrafish retinas. Multiple retinas from the  fgap43:egfp strain were dissciated and sorted for RGCs expressing GFP using a  Becton Dickinson FACSAria III sorter, 4 days post optic nerve crush. Although levels of endogenous Gap43 are low in the nave adult  retina, the GFP transgene tends to be long lived and is detectable by FACS at  levels that are above background but below the level of visual detection. Each replicate is derived from 50,000 pooled from biologically  distinct retinas. ATAC-seq libraries were prepared using the Tn5 transposase  system (Nextera DNA library kit, Illumina, FC-1211030) as previously  described by the Greenleaf lab and purified using DNA Clean &  Concentrator kit (SKU: ZD5205, Zymo).