16S rRNA amplicon sequencing of Homo Sapiens: patient rectal swab
Construction of the 16S rRNA amplicon library was based on the dual-index strategy, multiplexing 8 forward and 12 reverse primers. PCR amplification primers targeting the V3-V4 variable regions of the 16S rRNA gene were designed to contain a 58-bp adapter sequence specific for the Illumina MiSeq platform at the 5’, a 3 to 6-bp variability region, a 12-bp Golay barcode, and a 16S rRNA-gene specific primer sequence universal for Prokaryotes.