GSM3052839: WT_scRNA_batch1; Mus musculus; RNA-Seq
Bone marrow HSCs from WT or Med23-dificient adult mice were isolated by cell sorting (LSKsCD150+CD34-CD48-). Single mouse hematopoietic stem cells which were isolated by cell sorting, were manually picked, lysed and subjected to first-strand cDNA synthesis by using barcoded oligo-dT primers. Then, second-strand cDNA were synthesized, amplified and fragmented by sonication (Covaris). Final RNA-seq library was prepared by using the KAPA Hyper Prep Kit (KAPA Biosystems).
GEO Accession
GSM3052839