Illumina HiSeq 4000 paired end sequencing of SAMD00089386
Total RNA was extracted using the hot-borate method. An Illumina sequence library was constructed as described by Akagi et al. (2014, Science) from 10 mg of total RNA. After adapter ligation, libraries were size-selected using AMPure in the ratio of AMPure : reaction volume = 0.7 : 1. Quality of the library was assessed using the Fragment Analyzer.