Whole genome sequencing of Bombus impatiens: 8kb-insert mate-pair library
The library was constructed from a single adult male bee.  The hybrid 454/Illumina mate-pair library was prepared from 10ug of genomic DNA with the GS FLX-Titanium Paired-end Library Preparation kit following the manual (Roche Applied Sciences, Indianapolis, IN) but using library from the Genomic DNA Library Prep kit (Illumina). The library was sequenced using the SBS Sequencing kit V3 (Illumina).