GSM2871716: Fezf2+ IT-PN (4); Mus musculus; RNA-Seq
Animals were perfused with 0.9% Saline and the brain dissected out. Coronal sections (1 mm) were made using a TedPella matrix and layer 5 M1 of the right hemisphere dissected according to GFP(Fezf2) expression. Tissue was immediately dissociated into single cell suspension before FACS-purification of cells, separating cells from one animal into the Fezf2(GFP)+ CTB+ and CTB+ only cells, resulting in an average yields of 11,280 (n = 5) and 2,688 (n = 5) respectively. Cells were sorted directly into lysis solution from the RNAqueous micro kit (Thermo Fisher) and RNA extracted according to the manufacturers instructions. An ERCC spike-in was added to each RNA sample before the incorporation of a unique inline barcode in the initial cDNA preparation of each RNA sample step before PCR amplification. All samples were then pooled for in vitro transcription to further amplify RNA. Amplified RNA was then fragmented before reverse transcription and PCR amplification to generate the final cDNA library.
GEO Accession
GSM2871716