GSM3721570: CB CD34+ cells with FLT3-ITD-Replicate2; Homo sapiens; RNA-Seq
Total RNA was extracted using Trizol Reagent (Thermo Fisher Scientific) following the manufacture`s instructions. RNA quality was assessed by spectrophotometry(NanoDrop, ND1000) and gel electrophoresis. To be considered as high-quality, the RNA had to have the result of A260/A280≧1.8, A260/A230≧1.5, no gDNA contamination. RNA integrity was measured by Agilent RNA 6000 Nano Assay (RIN≧6). RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM3721570