GSM4928816: Len +EZH2i; Homo sapiens; RNA-Seq
Total RNA were isolated from the cell lines using the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen) according to the manufacturer's protocol One microgram total RNA was rRNA depleted using the Ribo-Zero rRNA Removal Kit (Human, Mouse, Rat) (Epicentre Biotechnologies, Madison, WI, USA) according to the manufacturer's instructions followed by a purification step using AMPure XP Beads (Beckman Coulter, Brea, CA, USA). Sequencing libraries were generated using the ScriptSeq v2 RNA-Seq Library Preparation Kit (Epicentre) according to the manufacturer's instructions using 12 PCR cycles.
GEO Accession
GSM4928816