Genome sequencing of gray wolves for de novo mutation rate estimation
All libraries were sequenced with a 100-bp paired-end strategy. Libraries for 302M and the four new individuals (480M, 629F, 645F, and 694M) were prepared as part of a previous study (Schweizer et al. 2018). Briefly, genomic DNA was sheared to ~300-450 bp using a Biorupter NGS Sonication System, then libraries were prepared using a with-bead protocol (Faircloth 2015) and unique 6 bp indexes (Faircloth & Glenn 2012).