GSM3660573: 25_019_NegCtrl; Homo sapiens; RNA-Seq
Total RNA was isolated using miRNeasy micro kit (Qiagen) loaded on an automated platform (Qiacube, Qiagen) and quantified as described previously (Seumois et al.Am J Clin Exp Immunol. 2012; PMID:23304658). Barcoded Illumina sequencing libraries (Nextera; Illumina) were generated utilizing an automated platform (Biomek FXP, Beckman Coulter). RNA-Seq - Sequencing platform, HiSeq2500 (Illumina). Libraries were sequenced to obtain more than 8 million 50-bp single-end reads (HiSeq Rapid Run Cluster and SBS Kit V2; Illumina).
GEO Accession
GSM3660573