GSM1199011: Myc ChIP sample 1 Lane 1 and Lane 2; Mus musculus; ChIP-Seq
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. 24 ug of sonicated chromatin was used for each IP. Sequencing libraries were generated for the two replicate chip samples using Illumina Tru-Seq ChIP-Seq kit, multiplexed to be sequenced in a single lane, according to the Illumina protocol for ChIP-Seq library preparation, with some modification. After adaptor ligation, PCR amplification was performed prior to size selection of the library [Schmidt D, Methods, 2009]. Clustering and 50-cycle single end sequencing were performed on the Illumina Hi-Seq 2000 Genome Analyzer. Two lanes of the multiplexed replicates were sequenced, and sequences from the two lanes were combined after alignment for each replicate.
GEO Accession
GSM1199011