GSM1654885: WT; Homo sapiens; OTHER
DNA from infected HT1080 cells was  extracted using Mammalian Genomic DNA miniprep kit (Sigma-Aldrich) Genomic DNA, digested with BfaI, BanII and CviQI, was ligated to a linker made by annealing synthetic oligonucleotides 5´- TAGTCCCTTAAGCGGAG (with 3´ end blocked by an amino group modifier) and 5´-GTAATACGAC TCACTATAGGGCNNNNNCTCCGCTTAAGGGAC (the string of random nucleotides comprise a serial number43, not relevant to this study). PFV U5 integration sites were PCR-amplified using primers 5´- CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCTGTAATACGACTCACTATAGGGC and 5´-GTGTGAACTACACTTATCTTAA ATGATG followed by a nested PCR with primers 5´- CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCTGTAATACGACTCACTATAGGGC and 5´- AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTXXXXXCTTAAATGATGTAACTCCTTAGGATAATCAATATAC (the string of underlined nucleotides marked with “X” denotes the position of a 5-base index, which was unique for each integration site library analyzed).
GEO Accession
GSM1654885