GSM4386815: Ht29OxR2; Homo sapiens; OTHER
10 colorectal cancer cell lines obtained from the ATCC (Rko, Ls1034, Dld1, Ls180, Colo205) and from the UNC Tissue Culture Facility (Colo320, Hct116, Ht29, Sw480, Ls174t) and grown according to ATCC specifications. For oxaliplatin treatments, cells were plated into R-150 plates and grown to about 90 percent confluence.  200μM oxaliplatin was added to each plate and cells were incubated at 32 degrees celcius for 2 hours.  Plates were then placed on ice and cooled cells were harvested by scraping and rinsing with ice-cold PBS. Cells were pelleted, transferred to Eppendorf tubes, and resuspended in 2mL of lysis buffer. Cells were lysed by homoginization and the soluble fraction was processed with RNase A and proteinase K.  Samples were immunoprecipitated with anti-XPG antibody, and then ligated to adapters and processed for sequencing (XR-seq).   Approximately 10 plates were used for each XR-seq assay.  After PCR amplification, libraries wrere sequencing on HS4000.
GEO Accession
GSM4386815