GSM2870458: MYCNoe_r4; Danio rerio; RNA-Seq
Sorted EGFP+ cells were sorted, then total RNA was extracted with Trizol-LS. Total RNA was depleted of ribosomal RNA with the SMARTer Universal Low Input RNA Kit (Clontech). Enriched mRNA was then applied to library preparation according to manufacturer's protocol (NEBNext Ultra). After repeated quality control for average DNA input size of 300 bp, the samples were sequenced on a HiSeq Illumina sequencer with 2x100bp paired-end reads.
GEO Accession
GSM2870458