wgs of E coli O157:H7 RM10716
Single Molecule, Real-Time (SMRT) Sequencing was performed using a Pacific Biosciences (PacBio)RS II platform using 20-kb SMRTbell libraries with P6/C4 sequencing chemistry, MagBead One Cell Per Well v1collection protocol, and 360-min data collection mode. DNA was extracted using wizard ( Promega) DNA extraction kit, sheared using a Corvaris g-tube, and libraries were prepared from 15 mg of bacterial genomic DNA, using the standard protocol, and processed for sequencing as recommended by the supplier.