GSM4212658: pH7_3; Rhizobium favelukesii; RNA-Seq
RNA Protect (Qiagen) reagent was added and cells harvested, the pellet was frozen in liquid nitrogen. The commercial kit RNeasy® Protect Bacteria Mini Kit (Qiagen) was used to the RNA extraction and DNA removed with DNase I (Qiagen). The depletion of the ribosomal RNA was performed with a commercial kit (Ribo-Zero rRNA removal kit, Illumina Inc.). The cDNA library was made with the commercial kit TruSeq® mRNA Sample Preparation (stranded) (Illumina Inc., San Diego, U.S.A.).
GEO Accession
GSM4212658