GSM2190901: rpoD_2; Escherichia coli str. K-12 substr. MG1655; RNA-Seq
RNA was extracted using hot phenol-chloroform. DNase treated RNA was depleted of ribosomal RNA using the Ambion Microbe Express Kit (AM1905). RNA was checked for quality using Bioanalyzer (Agilent). DNase treated RNA was depleted of ribosomal RNA using the Ambion Microbe Express Kit (AM1905). RNA was checked for quality using Bioanalyzer (Agilent). Checked RNA was used for library preparation and sequencing. Briefly, 100 ng of qubit quantified RNA was used for library preparation using the NEXTflex Rapid Directional RNA-Seq kit (5138-08 Bioo Scientific). The library was quantified using qubit and its quality was checked using Agilent Bioanalyzer before proceeding for sequencing on the Illumina NextSeq 500 sequencer.
GEO Accession
GSM2190901