Loki GC14 75 MDA-amplified DNA
DNA was extracted from 7,5 gram sediment using the FastDNA spin kit for soil in conjunction with the FastPrep-24 instrument (MP Biomedicals) following manufacturer’s protocol, except for the addition of polyadenosine as described by Hugenholtz et al. The individual extractions were then pooled and up-concentrated to a final volume of 50 µl using Amicon Ultra-0.5 filters (50.000 NMWL) following the manufacturer’s protocol (Merck Millipore). Due to low yield, and presence of inhibitors, genomic DNA was amplified using the REPLI-g ultrafast mini kit (Qiagen) according to the standard protocol for purified genomic DNA, and using 2,73 ng of the up-concentrated DNA as input. Nextera libraries (Illumina) were prepared according to the manufacturer’s instructions, using 50 ng of the amplified DNA as input.