RNA-Seq  Cnidopus japonicus tentacle (Red form)
The PCR-based cDNA library was, created following the instructions for the Mint-2 cDNA synthesis kit (Evrogen, Russia). Adapters PlugOligo-3M and CDS-4M used for cDNA synthesis. Ion Torrent sequencing — At the first stage of library preparation amplified cDNA (100 ng of each sample) was fragmented by 400-500 b.p. using Covaris S220 System (Covaris, Woburn, Massachusetts ,USA). After that, Ion Xpress™ Plus Fragment Library Kit (Life Technologies) was employed for barcode shotgun-library sample preparation. For conduction of emulsion PCR Ion PGM™ template OT2 400 (Life Technologies) kit was utilized. Sequencing was made in accordance with manufacturer protocol for genome analyzer Ion Torrent PGM (Life Technologies) utilizing Ion 318 chip and Ion PGM™ sequencing 400 kit v2 (Life Technologies).