GSM2870991: No mutation; Homo sapiens; OTHER
60h after nucleofection, cells were trypsinized, washed in PBS, and 500000 cells were lysed in 40µl of QuickExtract DNA Extraction Solution. 40µl of H2O was added to each lysis. PCR amplicons were purified on a 10% non-denaturing TBE/PAGE gel and the band corresponding to the PCR product was cut from the gel, eluted over-night, and precipitated with isopropanol. After resuspension, sample concentrations were quantified on a bioanalyzer, and the barcoded samples were pooled to a concentration of 0.4µM per sample in 10µl.
GEO Accession
GSM2870991