GSM3579197: Treated replicate 2; Escherichia coli O157:H7 str. Sakai; RNA-Seq
Enzymatic lysis was performed using TE buffer with proteinase K and 1 mg/ml lysozyme. RNA was then purified using the miRNeasy kit (Qiagen) with the inclusion of a double on-column DNA digestion. Ribosomal RNA was depleted using the Ribo-Zero bacterial rRNA removal kit (Illumina). Illumina TruSeqTM stranded mRNA kit (5 µl of rRNA-depleted sample were directly mixed with 13 µl of TruSeqTM 'Fragment, Prime, Finish mix'. Then, the kit guidelines were followed).
GEO Accession
GSM3579197