GSM3782936: 7-GC-MB-6586_S7; Homo sapiens; RNA-Seq
RNA libraries were prepared for sequencing using standard Illumina protocols total RNA was extracted using TRIzol Reagent. First strand cDNA synthesis was performed with SuperScript III First-Strand Synthesis System. After purification using SPRI beads, the double stranded cDNA was ligated to in-house designed adapters (based on TruSeq Indexed adapters (Illumina)) using NEBNext Ultra II (NEB) followed by 15 cycles of amplification and library purification