GSM3351310: DN1d-2; Mus musculus; RNA-Seq
Libraries for RNA-sequencing were prepared using the NuGEN Ovation RNA-seq v2 library preparation kit (NuGEN Technologies Inc.). Briefly, ~10,000 cells/subset were sorted (from 10 day old B6 mice) into the NuGEN Prelude Direct Lysis Buffer and total RNA was inputted directly into the first strand synthesis step of the Ovation RNA-seq v2 system. 1ng of SPIA amplified fragmented ds-cDNA was used to prepare libraries using the NuGEN Ovation Ultralow DR Multiplex System. Size distribution and qualitative assessment of libraries was performed using the Bioanalyzer DNA Chip 1000. Libraries were quantified using the KAPA Universal Library quantification kit prior to multiplexing and sequenced on the Illumina HiSeq platform.
GEO Accession
GSM3351310