GSM1356051: AX_2_WT; Dictyostelium discoideum; RNA-Seq
Small RNAs (< 200 nts) were extracted from 1 x 107 cells using the NucleoSpin® miRNA Kit (Macherey-Nagel) RNA samples were poly-A-tailed and the 5’PPP was converted into 5’P before an RNA adaptor was ligated to the 5’-monphosphate of the RNA. A poly-T-oligo was then used as primer for cDNA synthesis and the resulting cDNAs were PCR amplified.
GEO Accession
GSM1356051