GSM1841346: 5B6epid_1031; Mus musculus; RNA-Seq
Total RNA from each sample was isolated using Trizol (Invitrogen, Carlsbad, CA). Total RNA samples passing Agilent RIN greater than 7 were prepared for sequencing. Total RNA samples were prepared for sequencing using the SOLiD™ SAGE Kit using 500 ng input according to the manufacturer’s protocol. Emulsion PCR and SOLiD sequencing, SAGE-sequencing of 35 bp tags, 30 base pairs in a single direction, were performed according to manufacturer’s instructions for the SOLiD™ 3 System (Life Technologies). SOLiD SAGE Kit with Barcoding Adaptor Module (#4452811) and SOLiD RNA Barcode Kit (#4427046)
GEO Accession
GSM1841346