GSM3893152: vegetative mycelium, replicate 2; Auriculariopsis ampla; RNA-Seq
Total RNA was extracted using the Quick-RNA Miniprep kit (Zymo Research), following the manufacturer's protocol Library generation was performed using the TrueSeq RNA Library Preparation Kit v2 (Illumina) according to the manufacturer's instructions. RNA quality and quantity were assessed using RNA ScreenTape and Reagents on TapeStation (all from Agilent); only high quality (RIN >8.0) total RNA samples were processed. Next, RNA was DNaseI (ThermoFisher) treated and the mRNA was purified based on PolyA selection and fragmented. First strand cDNA synthesis was performed using SuperScript II (ThermoFisher) followed by second strand cDNA synthesis, end repair, 3'-end adenylation, adapter ligation and PCR amplification. Purification was done using AmPureXP Beads (Beackman Coulter). DNA concentration of each library was determined using the KAPA Library Quantification Kit for Illumina (KAPA Biosystems)