Metagenomic soil sample from PRAD 45B area, a 2-year-old rehabilitation site after iron ore mining from Corumba, MS, Brazil
Shotgun paired-end libraries were constructed using the QXT SureSelect (Agilent Technologies) kit according to the manufacturers' instructions. After, the libraries were pooled and the sequencing run was performed using a NextSeq 500 v2 kit high-output (150 cycles) in the NextSeq 500 illumina platform.