GSM1354733: SL26788; Homo sapiens; RNA-Seq
Exosomes were isolated from cell-free seminal fluid by ultracentrifugation over a 30% sucrose/D2O cushion. RNA was extracted using the Exiqon miRCURY RNA isolation kit ScriptMiner Small RNA-Seq Library Preparation Kit, according to the manufacturer’s instructions, except that following the RNA linker ligation, but before cDNA synthesis, the RNA was fractionated using a 10% acrylamide-urea gel. RNA fragments consisting of inserts of approximately 15-40 nt were excised as a lower band, and RNA fragments containing inserts of approximately 40-100 nt were excised as an upper band.
GEO Accession
GSM1354733