RNAseq of Photorhabdus asymbiotica temperature response
rRNA was depleted from RNA samples obtained from exponential-phase cultures   using the Ribozero kit for Gram-negative bacteria (Epicentre), and verified using   a 2100 Bioanalyzer with a RNA 6000 Pico kit (Agilent). Strand specific RNAseq   libraries were constructed using the Illumina compatible ScriptSeq mRNA-seq library   preparation kit (Epicentre). cDNA libraries were quantified using a 2100 Bioanalyzer   with a DNA 1000 kit (Agilent). The first replicate of   each library was sequenced on the HiSeq 2000 (Illumina), in two separate lanes with   100 bp paired-end reads at the University of Exeter Sequencing Service. The second replicate of each library was sequenced on the HiSeq 2000 (Illumina), multiplexed   with 10 libraries/lane with100 bp paired-end reads by Source Bioscience (UK).