Isoetes echinospora paired end RNA Seq: Leaf_01
Total RNA was extracted from root, corm and leaves tissues from c. 100 I. echinospora plants. Total RNA from leaves (two independent replicates), corm (two independent replicates) and rootlets (one replicate) was extracted with the RNeasy plant mini kit (Qiagen). On-column DNase I treatment was performed with RNase-free DNase I (Qiagen), according to the manufacturer s instructions. cDNA was synthesised with ProtoScript II reverse transcriptase (New England Biolabs) according to the manufacturer s instructions, using oligo (dT) primer.