RNA-Seq of bacteria: Sphingobacterium multivorum B-3
Total RNA was extracted by using Trizol reagent and rRNA wasremoved. The first strand cDNA was produced by reverse transcription using mRNA fragments as templates, reverse transcriptase and random primers. Then the second strand cDNA was synthesized using dUTP. After that, the second strand cDNA was digested by UNG enzyme, which was used for the construction of strand-specific library. Then the sequencing of the cDNA library was performed by an Illumina HiSeq Xten apparatus