Amplicon Sequencing of Environmental Samples: Activated Sludge Bioreactors
Isolated DNA samples were PCR amplified at the V4 variable region of 16S rRNA genes with primers 515F (5-GTGCCAGCMGCCGCGGTAA-3) and 806R (5-GGACTACVSGGGTATCTAAT-3) followed by bTEFAP Illumina MiSeq 2  300 bp barcoded amplicon sequencing.