GSM1841273: dilated cardiomyopathy 6; Homo sapiens; RNA-Seq
Tissue samples were homogenized in TRIzol reagent by TissueLaser Disruption system (Qiagen), flash frozen on dry ice, and total RNA was isolated according to manufacturer's protocol of TRIzol Reagent. RNA quality assessment was performed by Experion System (Bio-Rad) and 4 ug of total RNA was used for the construction of sequencing libraries by Illumina TruSeq RNA Sample Preparation Kit. cDNA libraries were prepared for sequencing according to TruSeq RNA Sample Preparation Guide. The quantity and quality of libraries were checked by Qubit (Life Technologies) and Experion Sistems (Bio-Rad).
GEO Accession
GSM1841273