RNA-seq of  Saccharomyces cerevisiae Y06240: evolved in YPD challenged in YPL, t100, replica 2
Total RNA was  depleted of ribosomal RNA using Ribo-Zero Gold rRNA removal yeast kit  (Illumina, WI). Indexed libraries RNAseq libraries were constructed using  strand-specific cDNA synthesis (TruSeq stranded mRNA preparation kit,  Illumina) from oligo-dT captured mRNAs, pooled in equimolar concentration and  subjected to single-end 75bp Illumina sequencing in an Illumina NextSeq500  platform using High Output 75 cycles kit v2.0 (illumina). All performed at  the Genomic core facility at SCSIE from University of Valencia (Valencia,  Spain)