Transcriptomics data of Pseudomonas aeruginosa biofilm treated with TeNR- Control 1
Next-generation sequencing library preparation was performed by following TruSeq RNA Sample Preparation v2 protocol (Illumina) with modifications: The mRNA purification step was omitted and instead, 200 ng of total RNA was directly added to the elute-fragment-prime step. The PCR amplification step, which selectively enriches for library fragments that have adapters ligated on both ends, was performed according to the manufacturer’s recommendation but the number of amplification cycles was reduced to 12. Each library was uniquely tagged with one of Illumina’s TruSeq LT RNA barcodes to allow library pooling for sequencing. Library quantitation was performed using Invitrogen’s Picogreen assay and the average library size was determined by running the libraries on a Bioanalyzer DNA 1000 chip (Agilent). Library concentration was normalized to 2 nM and the concentration was validated by qPCR on a ViiA-7 real-time thermocycler (Applied Biosystems), using qPCR primers recommended in Illumina’s qPCR protocol and Illumina’s PhiX control library as a standard.