GSM3271243: H4R3me2a_ChIPSeq rep1; Mus musculus; ChIP-Seq
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. MIN6 cells were fixed using 1% formaldehyde, lysed and sonicated using a Bioruptor (Diagenode). Immunoprecipitation was performed with anti-H4R3me2a (39705, Active Motif) or anti-CTCF (07-729, Merck Millipore) antibody. The 250 to 400bp genomic libraries were generated from the input and immunoprecipitated DNA and sequenced using Illumina NextSeq500 to generate 100bp paired-end reads.
GEO Accession
GSM3271243