RNA-seq of static culture-4
B. subtilis 168 was cultured in the fermentation medium in 250 ml flasks with 20 ml fermentation medium and grown at 40C with shaking at 220 rpm for 3 days. The cultured conditions were static under the same conditions without shaking. The total RNA of these strains was extracted, synthetic the first cDNA and DNA sequencing were performed on a HiSeq 2500sequencing system By the Beijing Genomics Institute