GSM1837418: NCBP2 replicate3; Homo sapiens; RIP-Seq
Cells were pelleted by centrifugation at 500 × g for 10 minutes at 4°C, washed twice with ice-cold PBS and lysed in an equal volume of RIP lysis buffer [10 mM HEPES pH 7.0, 100 mM KCl, 5 mM MgCl2, 25 mM EDTA, 0.5% (v/v) Nonidet P-40, 1 mM DTT, protease inhibitor cocktail (EDTA-free, cOmplete; Roche)] for 30 minutes on ice in the presence of 100 U/ml RNase inhibitor (Fermentas). RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM1837418