Metagenome of leaf microbiome: epiphytes and disease
For metagenomic sequencing, the DNA samples were fractionated by ultrasound into approximately 350 bp fragments, which were then used to construct the sequencing libraries using NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, USA), and then sequencing was conducted using the Illumina Novaseq 6000 Sequencer (Illumina, Inc., CA, USA).