GSM3207697: Gel 5' Multiplex Adapter AD MD  2; Homo sapiens; miRNA-Seq
RNA was isolated from 1 mL of plasma using the Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry Format) from Norgen Biotek using manufacturer's protocols. Libraries were constructed exactly as detailed in "Blocking of targeted microRNAs from next-generation sequencing libraries", Roberts et al, PMID:26209131, with modification to incorporate MAD-DASH treatment. Modification to Roberts et al. 2015 protocol involves a short 4-cycle PCR amplifcation followed by Ampure bead cleanup, MAD-DASH CRISPR RNP in vitro digestion of specific sequences such as adapter dimer for 2 hrs followed by treatment with RNAse A and Proteinase K to inactivate the reaction, then a second 11-cycle PCR to complete library construction before futher cleanup with Ampure Beads or denaturing gel extraction.
GEO Accession
GSM3207697