GSM1838558: ZF4-18d30dMeDIP; Danio rerio; MeDIP-Seq
For MeDIP-seq, genomic DNAs were isolated and sonicated. End repair, adenylation, adapter ligation, methylated DNA enrichment and methylated DNA library construction were all performed with The NEXTflex™ Methyl -Seq 1 Kit (BIOO LIFE SCIENCE PRODUCTS, Cat No. 5118-01 ) according to the manufacturer’s instruction. For RNA-seq, total RNA was isolated using TRlzol reagent (Invitrogen life Technologies). MeDIP DNA libraries were prepared with The NEXTflex™ Methyl -Seq 1 Kit (BIOO LIFE SCIENCE PRODUCTS, Cat No. 5118-01 ) according to the manufacturer’s instruction. RNA libraries were prepared for sequencing using standard Illumina protocols．
GEO Accession
GSM1838558