COI targeted sequencing of parasitoids infected caterpillars
2-step amplification procedure. First step with COI gene specific degenerate primers employing touch-down cycling profile. After cleaning PCR products second step PCR was done with MiSeq fused primers (adapters and identifiers) performing only 15 cycles. Subsequently PCR products were cleaned and concentration measured by Quant-iT kit. Afterwards samples were pooled in equimolar proportions to create a final library.