GSM3660946: 7.3 GFP+; Mus musculus; RNA-Seq
Lumbar spinal cord dorsal horns (bilateral) were dissected out of mice expressing Sun1-GFP fusion protein under the control of pDyn promoter.  Tissue was homogenized and filtered to liberate intact nuclei.  Nuclei suspensions were separated by FACS to yield GFP+ sample and GFP- sample from the same animal (5,000 nuclei each sample).  RNA from each sample was purified using RNAqueous micro kit (Thermofisher) and used for downstream RNA-Seq RNA libraries were prepared for sequencing using NuGEN SoLo RNA-Seq System v2, with 15 cycles of amplification RNA-Seq, Illumina HiSeq 1000
GEO Accession
GSM3660946