GSM4101155: ATACseq_H3K27ac_control; Rattus norvegicus; ATAC-seq
DNA for ATAC-seq: We isolated nuclei of 50,000 cells from the siControl and siCtcf rat Schwann cells in a cold lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630) by exclude GFP- and 7-AAD+ cells. After spinning down at 500 × g for 10 min at 4 °C, nuclei were resuspended in transposition mix containing TD (2× reaction buffer), TDE1 (Nextera Tn5 Transposase) at 37°C for 30 min. The samples were purified using a Qiagen MinElute kit. Transposed DNA fragments were subsequently amplified and purified using Qiagen MinElute PCR Purification Kit. Libraries were generated using the Ad1_noMX and Ad2.1-2.4 barcoded primers and were amplified for 11 total cycles. Libraries were purified with AMPure beads (Agencourt) to remove contaminating primer dimers. All libraries were sequenced on the Illumina HiSeq 2500 with 75 bp single-end reads. ATAC-seq libraries were purified with AMPure beads (Agencourt) to remove contaminating primer dimers. All libraries were sequenced on the Illumina HiSeq 2500 with 75 bp single-end reads.
GEO Accession
GSM4101155