GSM3351328: MEF-H3f3b-HA-NT; Mus musculus; ChIP-Seq
Cells were crosslinked with 1% formaldehyde for 10 min and nuclei were pelleted after sequential wash and resuspended in sonication buffer (0.1% SDS, 1mM EDTA, 10mM Tris (pH8.1)) and sonicated in a Covaris to shear the chromatin into 200-300 bp fragments. 50ug of chromatin was immunoprecipitated using aanti-HA antibody (Abcam). Immunoprecipitated chromatin was de-crosslinked and DNA purified. ChIP-seq libraries were constructed using a NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs). DNA sequence was performed by NextSeq system (Illumina).
GEO Accession
GSM3351328