RNAseq of P. vulgaris: roots inoculated with R. etli mutant strains: Sample P.vulgaris roots 24 HPI R. etli CE338
"Plant growth and inoculation were performed essentially as previously described (Blanco et al., 2009). Strains used in this study were Rhizobium etli strains : CE3, CE338, CE343, CE109 and nodA-. A minimum of 5 plants were inoculated with the indicated strains or YEM medium as control and roots were frozen in liquid nitrogen 24 hpi for RNA extraction. Harvested root tissue corresponds to the susceptible zone, which present growing root hairs and is susceptible to rhizobia infection. Two biological replicas were prepared independently."
alignment_software
TopHat2