Illumina MiSeq of Escherichia coli CCUG 62462
DNA from a frozen sample was obtained by plating on blood agar medium and incubating overnight. From the bacterial culture, DNA was isolated using PureLink® Genomic DNA kit (Invitrogen, Carlsbad, USA) according to the manufacturer’s description. The quality and quantity of DNA was assessed using NanoDrop ND 1000 spectrophotometer (Thermo Fisher, USA), Qubit 2.0 Fluorometer (Invitrogen, Singapore), and visualisation on agarose gel before sending DNA for WGS.