GSM4100367: 16: EXP1-EGM_smallRNA; Homo sapiens; miRNA-Seq
Total RNA for this study was isolated from samples using the Qiagen (Exiqon) miRCURY RNA isolation kit. Small RNAseq libraries were generated using the NEB Next SmallRNA library preparation kit according to the manufacturer's protocol, with some minor modifications. The reaction volumes were reduced to 1/5thof the recommended volume. Due to the low amounts of RNA input, the 3' SR adaptor, SR RT primer and the 5' SR adaptors were diluted 1:6 to avoid excessive amounts of free adaptors and the formation of adaptor dimers. The final libraries were purified using the Zymo DNA clean and concentrator-5 kit and subjected to size selection on the Pippin Prep with a cut off between 117 and 135 bp to deplete adaptor dimers and most of larger RNA species such as tRNA fragments.
GEO Accession
GSM4100367