Exome sequencing of an alveolar rhabdomyosarcoma cell line (RH30SJ)
DNA was extracted from the sample using the Qiagen Dneasy Kit according to manufacturer's directions. Two ug of high quality DNA was captured by hybridization using the Agilent SureSelectXT HumanAllExon 51Mb kit. Samples were prepared according to manufacturer's instructions. PCR amplification of the libraries was carried out for 6 cycles in the pre-capture step and for 10 cycles post-capture. Samples were barcoded and sequenced using the Illumina TruSeq SBS Kit v3. FASTQ files were generated by the CASAVA bcl2fastq scripts.