GSM3207668: 12_Msm_916_induced; Mycolicibacterium smegmatis; RNA-Seq
Biological triplicate cultures of M. smegmatis containing pDTCF-MSMEG_0916 in 7H9 media, were grown as 10 mL cultures in 50mL flasks, at 37ºC, 5%CO2, for 4 hours in the presence or absence of 100 ng/ml anhydrotetracycline. After harvesting, cells were spun down, resuspended in Trizol, and frozen at -80 until RNA isolation. Cell pellets in TRIzol were transferred to a tube containing Lysing Matrix B, and vigorously shaken at max speed for 30 s in a FastPrep 120 homogenizer three times. This mixture was centrifuged at max speed for 1 min and the supernatant was transferred to a fresh tube. RNA was isolated using the Direct-zol RNA MicroPrep kit (Zymol Research) according to manufacturer's instruction with on-column DNase treatment. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM3207668