RNA-seq on Campylobacter jejuni NCTC11168 grown in iron replete/limited conditions using miRNA protocol
Campylobacter jejuni NCTC11168 was grown either in iron replete ( 40 *M FeSO4) or iron limited (-40 *M FeSO4) MEMα. Total RNA was isolated as described previously using a hot-phenol chloroform method. RNA pellets were resuspended in TE buffer and treated with DNase I (Epicentre) to remove contaminating genomic DNA and PCR was performed to confirm the absence of DNA. Ribosomal RNAs were depleted using the RiboMinusTM transcriptome isolation kit from Life Technologies. The miRNA libraries were constructed using the standard protocol for Illumina miRNA sequencing libraries followed by standard cluster generation and sequencing. Sequencing adapters were then trimmed from the resulting reads.