GSM3580479: L4440_02; Caenorhabditis elegans; RNA-Seq
Embryos were isolated using bleaching and then incubated in M9 for three hours. Egg shells were disturbed with chymotrypsin/chitinase treatment. Embryonic cells were then homogenized by qiaShredder columns (Qiagen) following manufacturer's instructions. RNA was extracted using RNeasy micro plus kit (Qiagen) followng manufacturer's instructions. mRNA libraries were prepared by Stanford Genome Sequencing Center using TruSeq Stranded mRNA Library Preparation kit (Illumina).
GEO Accession
GSM3580479