Cockroach RNASeq
Heads of the insects (to minimize contamination by gut microbiota) were dissected, homogenized and total RNA was isolated using the TRIZOL reagent according to the manufacturer's instructions (Invitrogen, Carlsbad, CA). RNA was treated with DNaseI (Ambion) to remove DNA contamination and purified using Qiagen RNeasy Mini Kit. mRNA-Sequencing (mRNA-Seq) libraries were prepared using Illumina’s mRNA-Seq Sample Prep Kit (Illumina, San Diego, CA) following the manufacturer’s protocol. Barcoded libraries were multiplexed and sequenced as 50 bp-paired end reads on the HiSeq 2000 platform.