GSM3579690: RNAseq_MHCII_neg_keratinocytes_D14_Sepi_epidermis_rep3; Mus musculus; RNA-Seq
Keratinocytes were FACsorted from the epidermis of ear pinnae of C57BL/6 mice from naïve mice or day 14 post topical association with S. epidermidis NIHLM087. CD49f+Sca-1+ Keratinocytes were sorted as CD45-CD31-CD34- cells and further separated based on their expression of MHCII. RNA was extracted from 60 000 keratinocytes per replicate using the RNAeasy micro Kit for RNA Isolation (Qiagen), as per manufacturer's instructions. mRNA libraries were constructed using SMARTer Ultra Low Input RNA kit (Clonetech) + Nextera XT DNA library prep kit (Illumina). mRNA samples were pooled and sequenced paired-end for 75bp on 1 lane of a Illumina NextSeq500.
GEO Accession
GSM3579690