GSM1482475: ctl3; Mus musculus; RNA-Seq
Mice were anesthesized and sacrificed by cervical dislocation.  Hair was clipped and ventral skin dissected from the abdominal wall.  Skin was held in 0.25% trypsin-EDTA at 4C overnight, then incubated at 37C for 45 minutes.  The epidermis was removed to isolating the dermis.  The dermis was flash-frozen, mechanically homogenized, and lysed in Trizol.  RNA was extracted by standard Trizol protocol. Libraries were prepared in the CWRU Genomics Sequencing Core using TruSeq Stranded Total RNA kit (Illumina, San Diego, CA, USA; RS-122-2301).
GEO Accession
GSM1482475