"RNA-Seq of Homo Sapiens, sample SC_PENN_321: single cardiomyocyte cell"
"Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634). Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2100 Bioanalyzer to characterize DNA fragment size and concentration. The library was subjected to Ilumina sequencing platform. Reads were then demultiplexed by index sorting. "
alignment_software
STAR