Hordeum vulgare cold transcriptome
Library construction and sequencing were conducted as previously described in Dai et al., (2014). Briefly, the poly-A containing mRNA was enriched from total RNA by poly-T oligo-attached magnetic beads, and then fragmented into small pieces randomly. Using the fragments as templates, first-strand cDNA was synthesized with random hexamer-primers. Second-strand cDNA was synthesized using DNA polymerase I, dNTPs and RNase H. The products were amplified by PCR and purified after end-repairing and adaptor ligations with the IlluminaTruSeqTM RNA Sample Preparation Kit (Illumina, San Diego, CA).