GSM4700970: AB218; Rattus norvegicus; RNA-Seq
Whole brains were extracted and flash frozen in methylbutane chilled with dry ice. Frozen brains were subsequently coronally sectioned using a 1mm brain matrix. Sections were gently brushed onto slides and stored in -80°C.  . Bihemispheric tissue samples of the central nucleus/ basolateral (CeA/BLA) amygdala were collected for each subject using 1.50-mm tissue punches and stored for downstream processing. Punches were taken between approximately -2.6mm and -3.2mm from Bregma (28) RNA sequencing libraries were prepared using the KAPA Stranded RNA-Seq Kit with RiboErase (Kapa Biosystems) in accordance with the manufacturer's instructions and sequenced on an Illumina HiSeq2500 sequencer (v4 chemistry) using 2 x 50bp cycles.
GEO Accession
GSM4700970