GSM1193666: t0 ERG VCaP Input; Homo sapiens; ChIP-Seq
Briefly, 54 ul of ChIP DNA or 50 ng of total input control DNA were subjected to end-repair using T4 DNA polymerase, Klenow DNA polymerase and T4 polynucleotide kinase, before purification using the DNA Clean and concentrator-5 kit (Zymo Research). Adenine overhangs were added using Klenow 5¡¦-3¡¦exo-minus, Illumina Solexa sequencing adapters were ligated using T4 DNA ligase and amplified with 18 PCR cycles using Phusion DNA polymerase (Finnzymes) and Illumina Solexa sequencing primers 1.1 and 2.1. Libraries were size selected by electrophoresis, excising the DNA smear between 200-300 bp on a Dark Reader non-UV transilluminator, purified using a Qiagen gelextraction mini-elute kit and quantified using an Agilent Bioanalyser.
GEO Accession
GSM1193666