GSM4256509: single cell replicate 02; Mus musculus; RNA-Seq
Patch pipettes (1.5–2.0 MΩ) were filled with DEPC-treated internal solution containing (in mM): 140 K-methanesulfonate, 0.2 EGTA, 2 MgCl2 and 10 HEPES, pH-adjusted to 7.3 with KOH. RNase inhibitor (1U/μl, Ambion) was dissolved into the internal solution. Before filling the electrode with RNase inhibitor-containing solution (4.5 μl), the tip of the electrode was always loaded with a small volume of internal solution (~0.5 μl) to form a smooth seal. Immediately after establishing whole-cell mode, the cytosol of the recorded cell was aspirated into the patch pipette and immediately expelled into an RNase free 0.5-ml tube (Ambion). The cDNA libraries were prepared using the SMART-Seq® HT Kit (TAKARA Bio) and Nextera XT DNA Library Prep Kit (Illumina) as per the manufacturer's instructions.
GEO Accession
GSM4256509