sequences of bacteria
The four tillage methods were performed G1, G2, G3 and G4 (control). The soil samples were obtained from the L1, L2, and L3 soil depth. PCR products were then purified with Agencourt AMPure Beads (Beckman Coulter, Indianapolis, IN) and quantified using the PicoGreen dsDNA Assay Kit (Invitrogen, Carlsbad, CA, USA). After the individual quantification step, amplicons were pooled in equal amounts, and pair-end 2300 bp sequencing was performed using the Illumina MiSeq platform with the MiSeq Reagent Kit v3