GSM3716985: nintedanib.947178: Bleomycin + Nintedanib 2; Mus musculus; RNA-Seq
Singe cell isolation was completed using collagenase based tissue dissociation techniques.  Viable cells were collected and suspended in 2ml sterile PBS+0.4%BSA. Single cell suspensions were loaded on a Chromium Single Cell Instrument (10X Genomics) to generate single cell gel beads in emulsion. Single cell RNA-Seq libraries were prepared using version 2 Chromium single cell 3' chemistry. Sequencing was performed on Illumina NextSeq500 with a transcript length of 59 bp.
GEO Accession
GSM3716985