Rainbow Trout Head Kidney, Skamania Clonal Line
Total RNA was isolated from a Skamania Head Kidney (~1 year old) using a TRIzol extraction.  cDNA was synthesized using the SMARTer PCR cDNA synthesis protocol (Clontech) and then amplified using an Advantage PCR kit.  Purified (phenol-chloroform extraction) cDNA was then sequenced on a Roche 454 FLX Titanium Genome Sequencer.