Fz Uterine Swab
Quarter Horse mares were selected from healthy or diseased categories using stratified random sampling.  Five samples were collected by serially sampling the reproductive tract from cadual to cranial in order to avoid contamination.  Samples were collected from the clitoral fossa, vaginal vestibule, and vaginal vault using sterile, double guarded Equine Culture Swabs, and two uterine samples were collected, one by sterile swab and the other by low volume lavage with 150 milliliters of sterile saline.  Sequencing library preparation was performed as previously described in the protocol established by Kozich et al. 2013 using the barcoded SA5xx forward primers and the SB7xx reverse primers as listed. Sequencing was performed at Johns Hopkins University on the Illumina MiSeq using a v2 chemistry, paired-end, 250 base pair, 572 cycle reagent kit (Illumina).