Microbe sample from Clostridium autoethanogenum JA1-1
An UltraClean Microbial DNA Isolation Kit (MoBio, Carlsbad, CA) was used for DNA extraction from 10 mL serum bottle cultures grown with syngas as described above until late-exponential growth stage. Due to low initial DNA concentrations, the MoBio protocol was modified to include a 10-minute, 70 degree Celsius heat shock step followed by a 10 minute vortex step, and an additional 3 minutes bead-beating step to disrupt the cells. A NanoDrop 2000c UV-Vis spectrophotometer (ThermoSCIENTIFIC, Wilmington DE) was used to determine quality and concentration (20 -50 ng•µL-1) of isolated DNA. Samples were stored at -20°C until use.