GSM3271604: Donor D M2 Total MBD-Seq; Homo sapiens; MBD-Seq
DNA was extracted using the DNeasy Blood and Tissue Kit (Qiagen). Sonicated genomic DNA (target modal size 200-500 bp) was enriched for methylated DNA using the EpiMark Methylated DNA Enrichment Kit (New England Biolabs) according to manufacturer's protocol. A matched unenriched total input fraction was also analyzed for each sample. The enriched methylated and total input fractions were then subjected to NGS library preparation using the Thruplex library preparation kit (Rubicon).
GEO Accession
GSM3271604