FIE-HA Input binding in Arabidopsis thaliana
The FIE protein is the only Arabidopsis homolog of the ESC protein and is present in all active PRC2 complexes. Hence ChIP using the FIE protein as a target should identify all sites of PRC2 interaction with the genome. We carried out ChIP-seq with a FIE-HA protein complementing the fie-11 mutant in the C24 ecotype using aerial tissue of 12 day old seedlings.