GSM2870033: Female Knockout 1 Nanog; Mus musculus; RNA-Seq
Embryos were collected in 0,2ml PCR tubes and stored in lysis buffer (Absolutely RNA Nanoprep Kit, Stratagene #400753) at -80°C until further processed. Following thawing and according to the manufacturer's instructions, RNA from the E3.5 embryos was extracted using the Absolutely RNA Nanoprep Kit (Stratagene, #400753) and eluted in 5µl water For the 8 embryos, cDNA has been generated by SMARTer UltraLow from Clontech, according to manufacturer's protocol. We started with all available 3.5 µL total RNA per sample. cDNA fragment distribution and quantity has been assessed on the DNA HiSense chip on the 2100 Bioanalyzer (Agilent Technologies). Sequencing libraries have been prepared with the Illumina TruSeq DNA kit, according to manufacturer's protocol.
GEO Accession
GSM2870033