GSM1355137: set2del_kanmx_mnase; Saccharomyces cerevisiae; MNase-Seq
Cells were resuspended 20 ml of zymolyase buffer, and spheroplasts were made with 250 μg of zymolyase. The spheroplasts were spun down and resuspended in 2 ml NP buffer. Then, micrococcal nuclease (MNase) was added at a concentration from 40 U-100 U for 10 min at 37 °C. Digested chromatin was reverse-crosslinked with Proteinase K in 1% SDS and 10 mM EDTA solution at 65 °C overnight. After RNase A treatment, DNA was purified by phenol chloroform extraction followed by ethanol precipitation. Finally, DNA fragments of ~147 bp were size-selected with an E-gel system The NEB ChIP-seq library preparation for Illumina (New England BioLabs) were used
GEO Accession
GSM1355137