Amplicon seq of human OTC gene: xenografted FRG mouse liver
OTC locus was amplified from gDNA with sequence-specific primers hOTC intron8_F2 5'-AAAATGGCACCTCATCTTTGTT-3' and hOTC intron9_R2 5'-CCCCTGCTTATTTTATAGGGTCT-3' located outside of the homology region, to prevent amplification of the donor vector. Purified PCR products were used as template in a second nested reaction, using primers AA_82F 5-TTGCATTGATGTCTGACTACTGG-3 and AA_83R 5-TCCCCAAAGGCATGAATGACCAA-3