Illumina HiSeq 2500 sequencing; Time-series RNA-seq experiment performed on 10 melanoma cell lines treated with BRAF inhibitor vemurafenib.
Time-series RNA-seq experiment performed on 10 melanoma cell lines treated with BRAF inhibitor vemurafenib.
Cells are cultured in RPMI1640 medium. Medium was drug is replenished every 2days. The dose of vemurafenib for each cell line is the 2 times of the IC50 (half growth inhibition) value of each cell line. The dose of TNF is 1000IU/ml. RNA libraries were prepared for sequencing using standard Illumina protocols
Experimental Factor: cell line
M397
Experimental Factor: compound
NGFR (not described)
Experimental Factor: time
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