GSM4212711: Crist_muc_edge3; Cristatella mucedo; RNA-Seq
Two different types of morphological dissection were performed. In the first case, the colony was divided into central and peripheral (edge) zones. In the second case, elongated colonies were divided into middle and terminal parts. In case of statoblast differentiation, thawed statoblasts were allowed to grow in aquarium for the specified number of days, after which the whole colony was sequenced. The RNA was extracted using Zymo Research Quick RNA mini extraction kit (R1050, Zymo Research, Irvine, California, USA). For every sample, two biological replicates were made. Poly-A RNA fraction was enriched using oligo-dT magnetic beads. The libraries were prepared using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (E7760, New England BioLabs, Ipswich, Massachusetts, USA). Paired-end sequencing was carried out using Illumina HiSeq 2500, and single-end sequencing was done on Illumina NextSeq 500.
GEO Accession
GSM4212711