GSM4700630: E15-TD-51; Homo sapiens; RNA-Seq
The single-cell was washed in 30µL micro-drop of PBS-BSA solution and individual cells were picked into 0.2mL PCR tubes by micropipette for modified Smart2-seq,all the cDNA were quantified on a 2100 Bioanalyzer (Agilent) and Qubit 3.0. The scRNA-seq libraries were generated using the TruePrepTM DNA Library Prep Kit V2 for Illumina®(Vazyme) . Briefly, we use 5-50ng cDNA constructing the libraries according to the manufacturer's instructions.  cDNA molecules were preamplified and pooled followed by library construction. All libraries were quantified on a 2100 Bioanalyzer (Agilent) and realtime quantitative PCR, and then subjected to 150 bp paired-end sequencing on an Illumina Xten platform (sequenced by Novogene and Annoroad).
GEO Accession
GSM4700630