GSM3892316: ob1_9w; Mus musculus; miRNA-Seq
small-RNA extraction and purification was performed using the miRNeasy mini kit (Qiagen; 217004) following the manufacturers' protocol. Libraries for small-RNA sequencing were prepared using TruSeq Small RNA kit (Illumina; RS-930-1012) The concentration of indexed libraries was quantified by RT–qPCR using the Universal Kapa Library Quantification Kit (KAPA Biosystems). Final libraries were normalized and sequenced on an Illumina HiSeq 3000 sequencer.
GEO Accession
GSM3892316