GSM3661982: 921 rep3; Staphylococcus aureus; RNA-Seq
.  RNA was isolated using Tri reagent and chloroform and precipitated with isopropanol. RNA was isolated using the Qiagen RNeasy Mini Kit. Precipitated RNAs were treated with DNase I (ThermoFisher) following the manufacturer's instructions. Total RNA quality was assessed using the 2100 Bioanalyzer (Agilent). At least 200ng of DNase-treated total RNA with a RNA integrity number greater than 6 was used for rRNA depletion and generation of cDNA libraries via the ScriptSeq Complete (Bacterial) kit (Illumina/Epicenter) following the manufacturer's protocol. Library quality was assessed using the 2100 Bioanalyzer (Agilent) and libraries were quantitated using KAPA Library Quantification Kits (KAPA Biosystems). RNA sequencing was performed by the Vanderbilt Technologies for Advanced Genomics (VANTAGE) core using the Illumina HiSeq 3000 platform (Illumina).
GEO Accession
GSM3661982