GSM1195331: T-reg Bcatenin; Homo sapiens; ChIP-Seq
An iTreg pool of 5 different donors were crosslinked with 2 mM disuccinimidyl glutarate (Thermo Fisher Scientific) and 1% formaldehyde, cells were lysed in pre-immunoprecipitation buffer (10 mM Tris, 10 mM NaCl, 3 mM MgCl2 and 1 mM CaCl2). Chromatin was prepared and ChIP was performed according to the Millipore online protocol using 15 μg of β-Catenin antibody #9562 (Cell signaling technology, Boston, MA). Immunoprecipitated chromatin was sheared, end-repaired, sequencing adaptors were ligated and the library was amplified by ligation mediated PCR (LMPCR). After LMPCR, the library was purified and checked for the proper size range and for the absence of adaptor dimers on a 2% agarose gel.
GEO Accession
GSM1195331