PARE seq of barley: drought
Polyadenylated RNA was twice purified on oligo-dT(18) magnetic beads. Approximately 200 ng of polyA-enriched RNA were ligated to a an RNA adapter containing EcoP15I site. 26-27 mer 5 PARE libraries were synthesized according to Addo-Quaye (2009) and German (2009) protocols. The 12% PAGE purified libraries of 26-27-mer 5-ends of the 5-end phosphorylated mRNAs were sequenced