WXS, tumor tissue DNA from 48yo male
Whole-exome sequencing libraries were prepared using the SureSelectXT Library Preparation Kit (Agilent). DNA was sheared using an LE220 Focus-ultrasonicator (Covaris), and the fragments were end-repaired, adenylated, ligated to Illumina sequencing adapters, and amplified by PCR. Exome capture was performed using the SureSelectXT v4 51 Mb capture probe set (Agilent), and captured exome libraries were enriched by PCR. Final libraries were quantified using the KAPA Library Quantification Kit (KAPA Biosystems), Qubit Fluorometer (Life Technologies), and 2100 BioAnalyzer (Agilent) and were sequenced on a HiSeq2500 sequencer (Illumina) using 2  125 bp cycles.