GSM4101049: MCF10A cells, hG1a2; Homo sapiens; RNA-Seq
Cells were grown to 70% - 80% confluence, harvested by trypsinization and spun at 750 rpm for 3 minutes. RNA was isolated from cell-pellet using QIA shredder spin column and RNeasy Mini Kit according to manufacturer's protocol. 10 ng of isolated RNA was heat inactivated for 10 min at 80 °C followed by cDNA Synthesis using the SuperScript ® VILO™ cDNA Synthesis Kit. DNA library was generated using the Ion AmpliSeq™Transcriptome Human Gene Expression Kit according to manufacturers' instructions. Sequencing was performed on Ion S5 XL benchtop sequencer (Thermo Fisher Scientific) to a length of 200 base pairs. On average 8 million reads were sequenced per sample.
GEO Accession
GSM4101049