GSM1186520: 10% RWC; Dorcoceras hygrometricum; Bisulfite-Seq
DNA extracted from hydrated, moderate dehydration (70% RWC) and desiccated (10% RWC) leaf tissues of resurrection plant species B. hygrometrica. Fragment genome DNA to 100-300bp by Sonication, and then DNA-end repair , 3'-dA overhang and ligation of methylated sequencing adaptors are conducted. Bisulfite treatment by ZYMO EZ DNA Methylation-Gold kit. Desalting, size selection, PCR amplification and size selection again. Qualified library for sequencing.
GEO Accession
GSM1186520