Sequencing of fungal ITS rRNA from soil samples collected near and distant from subsurface drip irrigation emitter, one system installed in 2012 and the other in 2016.
Total DNA was extracted from 0.25g of soil from each experimental microcosm (n=60) using a PowerSoil extraction kit. DNA was amplified for the fungal internal transcribed spacer (ITS) region using primers ITS1F (Gardes and Bruns, 1993) and ITS4 (White et al., 1990). Sequencing libraries were constructed following the BASE protocol (Bisset et al 2016).