WGS of respiratory syncytial virus: nasopharyngeal specimens
The purified PCR products were quantified using Qubit and normalized to 0.2 ng/_L. Normalized DNA was tagmented and indexed using the Nextera XT. Barcoded libraries were then purified using 0.65X Ampure Xp beads. Bioanalyzer (Agilent) was used to confirm the expected size distributions and library quality. Each library was quantified using the Qubit then normalized and pooled at equimolar concentrations based on the Qubit results.