PygmyRattlesnakeFdna
S. miliarius livers from a male and a female were collected by Bob Walton and immediately placed in RNAlater, shipped on dry ice and stored at -80oC until RNA and DNA isolation. DNA was extracted from each sample with a Qiagen DNeasy Blood and Tissue kit, following the manufacturer’s protocol. Paired-end library preparation and sequencing were performed at the Beijing Genomic Institute.