GSM4386831: 295_0WT; Mus musculus; RNA-Seq
RNA was isolated using the miRNeasy kit from Qiagen according to the manufacturer's protocol. Purified RNA samples were then cleaned and resuspended in nuclease-free water using the RNeasy MinElute Cleanup kit from Qiagen according to the manufacturer's protocol. RNA quantity was measured using the Nanodrop 1000. Quality of RNA samples was quantitatively assessed by computation of RNA integrity numbers (RINs) using the Agilent RNA 6000 Pico kit (Agilent) following the manufacturer's protocol. RNA samples with RINs ≥ 7 were selected for RNA-sequencing (RNA-Seq). Libraries were then prepared from poly-A RNAs using the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific) according to the manufacturer's protocol for barcoded whole-transcriptome libraries. Size distribution of samples was assessed using the Agilent 2100 Bioanalyzer and 6000 RNA Pico kit. Library concentrations were quantified using the Agilent DNA 1000 assay and the 2100 Bioanalyzer according to the manufacturer's instructions. Reactions were loaded onto Ion PI Chips v3 (Thermofisher) for sequencing on an Ion Proton sequencer according to the manufacturer's protocol.
GEO Accession
GSM4386831