RNA-seq of Raphanus raphanistrum artificial floral selection lines
Approximately 100mg of early floral buds were collected for RNA extraction from four artificial selection lines - RH1, RL1, KH1 and KL1, with R and K corresponding to "Reed" and "KBS" lines and H and L corresponding to "High anther exsertion" and "Low anther exsertion", respectively. Plants from the 6th and the 7th generation of the R and K lines, respectively, were sampled. For more details, please refer to PMID: 21956022. Total RNA was purified using Qiagen RNEasy and tested for quality using Bioanalyzer. PolyA RNA was further sequenced using Ilumina Genome Analyzer, yielding 36bp reads.