GSM2617099: cre1; Mus musculus; RNA-Seq
Hippocampi were then dissected and snap frozen with liquid nitrogen in 1mL Trizol and stored at -80°C. For RNA isolation, samples were thawed in a 37°C waterbath then kept on ice prior to homogenization with a Polytron homogenizer with a 5 second pulse. After a 5 minute incubation at room temperature, one tenth the sample volume of 1-bromo-3chloropropane was added, mixed by inversion and a 3 minute incubation at room temperature. Samples were then centrifuged at 13,000 X g for 15 minutes at 4°C to separate the aqueous and organic layers. As much of the aqueous layer was recovered as possible, then RNA was precipitated with isopropanol. Pellets were then washed with 75% ethanol and resuspended in 50µL deionized water. RNA library preparation and sequencing were performed by HudsonAlpha. RNA was Poly(A) selected and 300bp size selected. Libraries were sequenced for 25 million 50bp paired end reads.
GEO Accession
GSM2617099