GSM4212999: 14_S14_L002; Rattus norvegicus; RNA-Seq
VTA tissue punches were homogenized in Trizol (Thermo Fisher), and RNA was isolated on RNeasy Microcolumns (Qiagen) following manufacturer's instructions. RNA concentrations were assessed using a nanodrop spectrophotometer and RNA 260/280 and 260/230 ratios were confirmed at >1.8. Following RNA purification, RNA-seq libraries were prepared according to Illumina protocols and sequenced on an Illumina HiSeq 4000 (or equivalent) sequencer.
GEO Accession
GSM4212999