GSM3579486: PSPAO1_Rep2; Pseudomonas aeruginosa PA14; RNA-Seq
Total RNA was isolated from 5mL-worth of cell pellets using TRIzol (Life Technolgies) following the manufacturers instructions. Once isolated, RNA was treated with Rnase-free DNAse I (Qiagen) for 1 hour to remove residual DNA and then cleaned and concentrated using the RNeasy kit (Qiagen) . Libraries were constructed using NEBNext® Ultra™ Directional RNA Library Prep Kit from Illumina. 50 base pair single end sequence reads were generated using the Illumina HiSeq 2000 platform at the Vienna BioCenter Core Facility (http://www.csf.ac.at)
GEO Accession
GSM3579486