GSM2528013: rib_KO_Th1_4h_2; Mus musculus; OTHER
ribosome profiling Ribosome protected fragment RNA was ligated to 3' adapter, then size-selected by urea PAGE.. RNA was reverse transcribed to cDNA with indexed RT primers in the presence of bromodeoxyuirdine. cDNA was then  captured on beads with anti-Br-dU antibody and circularized with CircLigase to position adapter sequences on the 5' end. Libraries were then PCR-amplified using primers with Illumina adapters.  Please see accompanying protocol for details.
GEO Accession
GSM2528013