RNA-seq of tomato: green ripening fruit
Total RNA was extracted from the fruits using EasyPure Plant RNA Kit (Transgen, China)according to the manufacturer protocol. The RNA concentration and purity were measured using an NanoDrop 2004 (Thermo Scientific, Waltham, England). The RNA integrity was checked by agarose gel electrophoresis. Genomic DNA was removed from extracted total RNA by DNase treatment.