BV2_RNAseq_2hr_LPS_R2
Total RNA was extracted using TRIzol® (Life technologies) according   to the manufacturer protocol. For RNA-Seq, RNA libraries were created from each group   using NEBNext® UltraTM Directinal RNA Library Preap kit for Illumina®. The first step in   the workflow involved the removing of ribosomal RNA using RNAMiusTM Transcriptome   Isolation kit (Lifetechnology). Following purification, the total RNA was fragmented into small   pieces using divalent cations under elevated temperature. The cleaved RNA fragments   were copied into first strand cDNA using reverse transcriptase and random primers and   followed by second strand cDNA synthesis using DNA polymerase I and RNase H. The   cDNA fragments were then processed through an end repair reaction by the addition   of a single ‘A’ base, followed by ligation of the adapters. The products of these reactions   were then purified and enriched with PCR to create the final cDNA library. The cDNA   fragments were sequenced with HiSeq2500 (101 cycles PE lane) of Illumina Inc.