GSM1199551: RNA-seq of PolyA minus from  Mouse Ter119+ erythroid  cells; Mus musculus; RNA-Seq
RNA was isolated with Tri-Reagent (Sigma). The RNA quality was assessed using Agilent Bioanalyser. Only RNA with overall RIN score >9 was used for further procedures. Subsequently, RNA was treated with DNAseI (DNA-free, Ambion) according to the manufacturer’s protocol kit according to the protocol. Good quality and DNA-free RNA was subjected to polyA fractionation using PolyA selection kit (Promega) using the standard kit protocol.  The RNA fraction was precipitated overnight and resuspended with water. The quality of obtained RNA samples was assessed using PicoChip (Agilent). A Paired sequencing library was generated using the standard Illumina prtocol. For RNA-Seq libraries, total RNA was split into poly(A)+ and poly(A)- RNA using the PolyATract mRNA isolation system (Promega).  Poly(A)- RNA libraries were generated using the ScriptSeq v2 RNA-Seq Library Preparation Kit (Epicentre), after depletion of ribosomal transcripts with RiboMi- nus Eukaryote Kit for RNA sequencing (Invitrogen and sequenced on Illumina  GA-IIx.
GEO Accession
GSM1199551