GSM4211334: 44265_P2 TFH; Macaca mulatta; RNA-Seq
CD4 T cell subsets were sorted from frozen lymph nodes. Collected cells were flash frozen in RLT buffer and extracted using the Qiagen RNA isolation kit according to manufacturers instructions. RNA converted to ds DNA. dsDNA fragmented the ends are repaired and 'A' tailed, adapters are ligated on, size selection is carried out, then PCR performed to generate the final library ready for sequencing according to Illumina protocols 3′-Tag-Seq (QuantSeq) Gene Expression Profiling
GEO Accession
GSM4211334