GSM1195694: Rag1KO DN rep1; Mus musculus; RNA-Seq
RNAfrom thymuses (4 week old mice) was harvested using ambion Ribopure kit. 1 ug of total RNA for the construction of sequencing libraries. Ribosomal RNA was removed by a hybridization method using Ribo-ZERO kits (EpiCentre). mRNA was then fragmented and reverse transcribed to yield double stranded cDNA. cDNA was blunt ended, had an A base added to the 3’ ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 12 cycles using primers incorporating unique index tags. Fragments were sequenced on an Illumina HiSeq-2000 using paired end reads extending 101 bases.
GEO Accession
GSM1195694