Microbe sample from Clostridium ljungdahlii PETC
An UltraClean Microbial DNA Isolation Kit (MoBio, Carlsbad, CA) was used for DNA extraction from 10 mL serum bottle cultures grown with syngas as described above until late-exponential growth stage. Due to low initial DNA concentrations, the MoBio protocol was modified to include a 10-minute, 70 degree Celsius heat shock step followed by a 10 minute vortex step. A NanoDrop 2000c UV-Vis spectrophotometer (ThermoSCIENTIFIC, Wilmington DE) was used to determine quality and concentration (20 -50 ng•µL-1) of isolated DNA. Samples were stored at -20°C until use.