GSM3769142: Donor No.8 SFC 24h; Homo sapiens; RNA-Seq
Total RNA was isolated using Qiagen RNeasy Minikits (Qiagen) according to the manufacturer's recommendations. Next, RNA integrity and concentration were assessed using an Agilent Bioanalyzer 2100 (Agilent) and Nanodrop Spectrophotometer respectively. cDNA libraries were generated using a SciClone NGS Library Production Robot (Perkin Elmer) and library quality was verified using the Agilent Bioanalyzer. RNA sequencing was subsequently performed using an Illumina HiSeq2500 system (Illumina, San Diego, CA, USA) to achieve single 100 base pair reads with a read depths of 15-20 million reads per sample.
GEO Accession
GSM3769142