GSM3662052: HiC_Sis_orc1_1_rep1; Sulfolobus islandicus; Hi-C
Cells were crosslinked, permeabilized, and treated with HindIII to digest cellular DNA. The DNA was end-labeled with biotin and subjected to priximity ligation. After reversal of crosslinks, the DNA was extracted with phenol:chloroform:isoamyl alcohol, precipitated in isopropanol, and sonicated. Libraries were constructed using NEBNext Ultra DNA Library Prep Kit for Illumina. Biotin-labeled DNA was purified with streptavidin before PCR. Sequencing was performed on the Illumina NextSeq 500 platform.
GEO Accession
GSM3662052