GSM2797444: N7BY; Apis cerana cerana; RNA-Seq
Total RNAs were extracted from the heads of 2 nurse samples (N7BY and Tq22BY) and 3 forager samples (N18CJ, N22CJ, Tq7CJ) respectively using the guanidinium-HCl procedure described in Sambrook et al. (1989) (31). Potential DNA contaminants were removed using DNA-free kits from Ambion. RNA purity and integrity were analyzed using a NanoPhotometer® spectrophotometer (IMPLEN, CA, USA) and an Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA). RNA concentration was measured using Qubit® RNA Assay Kit in Qubit® 2.0 Flurometer (Life Technologies, CA, USA). RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM2797444