GSM4928798: KP19002; Homo sapiens; RNA-Seq
Enriched human CD33+ cells from liver and spleen of hNSG-SGM3 mice were resuspended in PBS containing 0.04% BSA and the cell numbers were counted on Contess II automated cell counter (ThermoFisher), and approximately 12,000 cells were loaded per channel on Chromium microfluidic chips (10x Genomics). Single cell capture, barcoding and library preparation were performed using the 10X Chromium version 2 chemistry according to the manufacturer's protocol (10x Genomics).  The quality of cDNA and libraries were checked on an Agilent 4200 TapeStation, quantified by KAPA qPCR, and sequencing on a HiSeq 4000 (Illumina) to an average depth of 50,000 reads per cell.
GEO Accession
GSM4928798