GSM4700014: UL_Rep1; Drosophila melanogaster; RNA-Seq
Fly heads were dissected and frozen by liquid nitrogen. Total RNA was isolated from the pooled fly heads at the age of day 30 using RNAzol®RT (Sigma). RNA-sequencing was carried at BGI (Hong Kong) according to company's protocol. RNA quality was assessed with Agilent 2100 bioanalyzer. Following DNAse I treatment, mRNA enriched by magnetic beads coupled to Oligo-dT was fragmented and used as template for cDNA library construction. Short DNA fragments were then subjected to end-repair, A-tailing, adaptor ligation, size selection and sequenced on BGISEQ PE100.
GEO Accession
GSM4700014