GSM3351687: HEPS_AND1_AF4MLL_Sample2_Rep3; Homo sapiens; RNA-Seq
HEPs were FACS-purified from day 15 EBs of EV or MA4-A4M expressing cells into individual wells of a 96-well PCR plate containing lysis buffer (0.2% Triton-X100 and 2.3 U of SUPERase-In RNase Inhibitor; Ambion). Three independent replicates (50 cells/replicate) per condition were analyzed. RNA/cDNA was obtained and amplified as per the SMARTSEQ2 protocol. Single-cell RNA-seq analysis was performed as described previously (Picelli et al., 2014). Pooled libraries were sequenced on the Illumina HiSeq 4000 platform. Libraries were prepared for sequencing using the Illumina Nextera XT DNA preparation kit.
GEO Accession
GSM3351687