GSM3892355: KC_ob3_9w; Mus musculus; RNA-Seq
Total RNA extraction and purification was performed using the TRIzol Reagent (ThermoFisher Scientific; 15596018) following the manufacturers' protocol. RNA integrity was determined using an Agilent Bioanalyzer. Libraries from mouse RNA were prepared using TruSeq Stranded mRNA kit (Illumina; RS-122-2201). The concentration of indexed libraries was quantified by RT–qPCR using the Universal Kapa Library Quantification Kit (KAPA Biosystems). Final libraries were normalized and sequenced on an Illumina HiSeq 3000 sequencer.
GEO Accession
GSM3892355