GSM2871732: Transgenic cells -NAEK 02_RNA-seq; Homo sapiens; RNA-Seq
Transgenic cells, either in the present of absent of NAEK (48h), were lysed with Trizol and total RNA was extracted following the manufacturer's instructions. PolyA+ RNA was selected on oligo(dT) beads (Life Ltd.) and fragmented in Fragmentation Buffer (NEB Ltd.). RNA fragments were then reverse-transcribed into cDNA and purified on TBE gel. Purified cDNAs were end repaired, ligated with adapters and picked out with a mean size of roughly 200bp. Libraries were built by PCR amplification and further purification by AMPure XP beads.
GEO Accession
GSM2871732