GSM1482874: CO2; Boehmeria nivea; RNA-Seq
After twenty days the ramie suffering from Cd stress, two CO and two CdS plants were sampled as two CO and CdS replicates for Illumina sequencing. The whole plant’s tissues for each plant, including root, stem, leaves and shoot, were sampled as one replicate. The sampled tissues were immediately frozen in liquid nitrogen and stored at -80° until use. Total RNA of each sample was extracted using an E.Z.N.A. Plant RNA Kit (OMEGA Bio-Tek, USA) according to the manufacturer’s protocol. RNA of four samples were separately used to construct the cDNA library with a fragment length range of 200 bp (±25 bp). Then, the paired-end sequencing was performed by using the Illumina sequencing platform (HiSeqTM 2000) according to the manufacturer’s instructions (Illumina, San Diego, CA).
GEO Accession
GSM1482874