Rice root on putting soil inoculated with Rhizobium leguminosarum bv. trifolii strain E11
Ten ng of each DNA sample were used as template in PCR with the following final concentrations of reagents: 400 nM 563F (forward) primer, 400 nM 924R (reverse) primer, 1X FastStart High Fidelity PCR Buffer (Roche, Cat # 03 553 400 001), 0.5 U of Taq polymerase (Roche), 200 mM dNTPs. Each reaction was set up in triplicate and amplified using the following cycle: 94 C/3 min, followed by 30 cycles at 94 C/45 sec, 56 C/45 sec, and 72 C/1 min, and a final extension at 72 C/7 min.