GSM2871619: 347P1; Homo sapiens; RNA-Seq
Endoneurial microvessels were fluorescently detected in 20 micron thick axial cryosections of human sural nerve biopsies using UEA-1-FITC, and laser capture microdissection performed. Microvessels were stored in extraction buffer at -80oC and RNA isolated using Arcturus Pico Pure Isolation Kit (Thermo Fisher Scientific) or miRNeasy kit (Qiagen, Hilden, Germany). On-column DNase digestion was also performed to remove contaminating genomic DNA using the RNase-free DNase Set (Qiagen). cDNA libraries were generated using the SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian (Clontech) and ribosomal cDNA was depleted prior to the final amplification step
GEO Accession
GSM2871619