Comparative transcriptome profiling of the fertile and sterile flower buds of sesame
cDNA libraries from sterile buds were constructed. From approximately 5mg of total RNA, mRNA was isolated, fragmented, converted to cDNA, and PCR amplified according to the Illumina RNA-Seq protocol (Illumina, Inc. San Diego, CA). Sequence reads were generated using the Illumina Genome AnalyzerⅡ (SanDiego, CA) and Illumina HiSeq 2000 (SanDiego, CA) at the Beijing Genomics institute (ShenZhen, China).