metagenome of amended Australian coal well
Metagenome sequencing of the 25 month DNA sample was done on the IonTorrent PGM. All library preparation and sequencing steps were performed according to the manufacturers instructions. 100 ng genomic DNA was sheared in four one minute shear one minute cool down cycles using a BioruptorŽ Sonicator (Diagenode, Denville NJ, United States). The library was prepared using the Ion Plus Fragment Library Kit protocol. Size distribution and concentration of the library was determined using the Agilent 2100 Bioanalyzer (Agilent Technologies Inc., Santa Clara CA, United States). The library was prepared for sequencing using the Ion OneTouch Template 400 bp Kit. Sequencing was performed using the Ion 318 Chip Kit v2.