16S rRNA V4 from coral reef sediment (SampleID: O_SD19): no peptide nucleic acid clamp
Microbial DNA extracted from 0.25g of reef sediment. DNA extracted using Qiagen PowerSoil kit following manufactuer protocols. Amplified using 515F-806R primers for 16S rRNA (V4 region). No PNA clamps were added to the PCR reaction.