RH641_early
Total RNA was extracted from liver biopsy specimens using the Invitrogen RiboPure kit (ThermoFisher, Waltham, MA). mRNA was isolated with NEBNext Poly(A) mRNA Magnetic Isolation kit (New England BioLabs, Ipswich, MA). NEBNext Ultra II RNA Library Prep kit for Illumina (New England BioLabs) was used to generate RNAseq libraries according to manufacturers protocol.