GSM3721973: A250_ATAC; Homo sapiens; ATAC-seq
48 hours after siRNA transfection, nuclei were isolated in cold lysis buffer (10mM Tris-HCL [pH 7.4], 10 mM NaCl, 3mM MgCl2, 0.1% NP-40) by centrifugation at 500Xg for 10 min. ATAC-seq libraries were prepared using the Nextera DNA Library Prep Kit, puified using the Qiagen MinElute Reaction Cleanup Kit, amplified by PCR and purified using Kapa Pure Beads.
GEO Accession
GSM3721973