GSM4255650: RF-Mock-D3-1; Homo sapiens; RNA-Seq
Cells were collected by trypsinization (Thermo Fisher #25300054) and pelleted by centrifugation. Pellet was washed in phosphate-buffered saline and RNA was extracted using a Qiagen Rneasy Mini Kit (Qiagen #74106) following the manufacturer's instructions. Samples were submitted to the BU Microarray and Sequencing Core for library preparation and polyadenylated RNA selection using Kapa RNA HyperPrep kit with Riboerase, and sequenced yielding 2x75 bp paired-end read datasets. Read library quality was assessed using FastQC (Andrews, 2015) and MultiQC (Ewels, Magnusson, Lundin, & Käller, 2016) packages. Illumina adapters were removed and leading and trailing low quality bases (below quality 30) were trimmed using Trimmomatic (Bolger, Lohse, & Usadel, 2014). Reads which were less than 36 bases long after these steps were dropped.
GEO Accession
GSM4255650