GSM3577870: AB5317; Mus musculus; RNA-Seq
Mice were intracardially perfused with PBS. Brains were dissected, dissociated using the gentleMACS™ dissociator (Miltenyi Biotec), and loaded on a Percoll gradient (GE Healthcare) to isolate leukocytes. Cells were collected, filtered through a 70µm cell strainer, washed with FACS buffer and maintained strictly at 4°C. Cells were stained with FACS antibodies, then sorted into 384-well plates Single-cell RNA-seq libraries were prepared as previously described {Jaitin, 2014}. In brief, mRNA from single cells sorted into capture plates were barcoded and converted into cDNA and then pooled using an automated pipeline. The pooled sample was linearly amplified by T7 in vitro transcription, and the resulting RNA was fragmented and converted into a sequencing-ready library by tagging the samples with pool barcodes and Illumina sequences during ligation, RT, and PCR. Each pool of cells was tested for library quality and concentration as described previously {Jaitin, 2014}
GEO Accession
GSM3577870