GSM2871599: Blood-derived immune-cell mixture from donor 1; Homo sapiens; RNA-Seq
The PBMC fraction was collected and washed three times with Dulbecco's phosphate buffered saline. To isolate polymorphonuclear (PMN) cells, the cells on top of the erythrocytes were collected and contaminating red blood cells were removed by two rounds of lysis with 0.2% NaCl solution at 4°C. PMN were added to the PBMC fractions in low abundance (3-6% of total cells) and aliquots were taken for RNA extraction and flow cytometry analysis. Total RNA was extracted with the Qiagen RNeasy mini kit (Qiagen GmbH, Hilden, Austria), including on-column DNAse I treatment, and subjected to INVIEW polyA RNA library preparation. Library preparation incorporated adaptor sequences and indexing compatible for Illumina sequencing technology, using proprietary methods of GATC Biotech (https://www.gatc-biotech.com).
GEO Accession
GSM2871599