GSM1354510: chd1ChIP_wt_rep1; Saccharomyces cerevisiae BY4741; ChIP-Seq
Cross-linked chromatins were sheared using a sonicator, and immunoprecipitated using the following beads or anti-body:anti-Myc conjugated agarose bead (Sigma Aldrich, cat.# E6654) to pull-down Chd1 in the Chd1-13XMyc strains, and RNAPII Ser5P/Ser2P antibody (Abcam, cat.# ab5131 and ab5095) to pull-down elongating RNAPII. The NEB ChIP-seq library preparation for Illumina (New England BioLabs) was used to sequence the samples using Illumina HiSeq 2000.
GEO Accession
GSM1354510