Naso elegans sample01 gut microbiota
A fragment of 16S rRNA gene targeting the V3-V6 region was PCR amplified using the primer pair B343F (forward) and B1099rc (reverse; Liu et al. 2007) with FLX Titanium adaptors (Lib-L) and a domain-specific primer fused with a two-nucleotide linker and sample-specific 8-bp barcode for multiplexing (HSPL-purified; Sigma-Aldrich Life Science, MO, USA). Each sample was PCR-amplified in triplicates, purified using the Zymoclean gel DNA recovery kit (ZYMO Research, CA, USA). The purified amplicons were quantified with Quanti-iT PicoGreen (Life technologies, CA, USA) and pooled at equimolar concentrations prior to sequencing on a Roche 454 GS FLX Titanium platform.