GSM1842489: hst1Δ-KO1 rep1; Clavispora lusitaniae; RNA-Seq
Cells were resuspended in 0.5 ml AE buffer with 10% SDS, 0.5 ml phenol, pH 5.3, and approximately 0.3 ml glass beads.  Samples were vortexed 30 min at 4°, incubated 10 min at 65°, frozen at -80°, and spun 10 min in microcentrifuge at room temperature.  The aqueous layer was extracted twice with phenol/CHCl3, and the RNA was precipitated with ethanol.  100 ug RNA was treated with DNase I for 10 min at room temp, applied to RNeasy spin column (Qiagen), treated with DNase I on the column, and eluted in water. RNA libraries were prepared for sequencing using standard Illumina protocols at the Duke University Sequencing core facility
GEO Accession
GSM1842489