Deep sequencing of the viral phoH gene throughout the water column: March 2011 400m
Viral DNA was extracted from environmental samples and amplified by whole genome amplification. The phoH gene was amplified with viral phoH primers and tagged using barcoded primers in a reconditioning PCR step. PhoH amplicon sequencing was performed on the 454 GS FLX Titanium platform. Using mothur, the sequences were grouped into operational taxonomic units (OTUs) defined by sequence identity of 97% or greater. This file contains phoH sequences representative of 94 OTUs: the 51 OTUs that contain at least 0.1% of the total number of sequences, and an additional 43 OTUs that contain at least 1% of the sequences from any individual sample. Raw, demultiplexed data were aligned to the representative OTUs by NCBI using bowtie2.