GSM3721265: RMS386; Mus musculus; RNA-Seq
Cells were loaded onto C1 integrated fluidic circuits IFC (5- to 10-μm chip) for cell lysis, reverse transcription with oligo (dT) primers and amplification of cDNA on a C1 Single Cell Auto Prep System according to the mRNA-seq protocol of the manufacturer (Fluidigm). Only cells with high-quality cDNA were processed for subsequent library preparation. A Nextera_XT Kit (PN FC-131-1096; Illumina) with dual indices (PN FC-131-1002; Illumina) was used for the preparation of single-cell multiplexed libraries, which were sequenced as 101-bp single-end reads on the Illumina HiSeq 2000 platform (96 samples/lane).
GEO Accession
GSM3721265