GSM2626834: sMOsrsf5a_rep2; Danio rerio; RNA-Seq
For each conditions, 80 (previously dechorionated) embryos were euthanized at 48hpf with tricaine (at the recommended dose). Embryos were lysed and homogenized in TRIzol Reagent (Sigma Aldrich) using a G22 and G26 needle. RNA were extracted using chloroform and precipitated with isopropanol. Another purification stage was performed using the Rneasy mini kit (Qiagen). True Seq Kit (Illumina). 1Μg of RNA was used for library construction.
GEO Accession
GSM2626834