GSM3660749: H3K27me3_AC_rep1; Malus domestica; ChIP-Seq
Preparation of chromatin samples and immunoprecipitation were performed as described previously (Gendrel et al., 2005). Three to four flower buds were used as one replicate. Cross-link was conducted as described previously (Saito et al., 2015) with minor modifications. In brief, each flower bud was first cut longitudinally by razor and divided into 8 equal parts. The immunoprecipitated chromatin samples were amplified and used for library construction using the ThruPLEX DNA-seq Kit (Takara Bio USA) as per the manufacturer's instructions.
GEO Accession
GSM3660749