Mut secondary Fb3 mtDNA
Mitochondrial DNA was amplified from the genomic DNA using REPLI-g Mitochondrial DNA kit (Qiagen, Hilden, Germany), which amplifies circular DNA. After the enrichment the amplified mtDNA samples were processed to Illumina sequencing compatible libraries with Nextera DNA sample preparation kit (Illumina, San Diego, CA, USA).The libraries were incubated with EdgeBio SOPE Resin and purified with EdgeBio Performa plates. After purification the samples with different index tags were pooled together and concentrated with DNA Clean & ConcentratorTM-5 (Zymo Research, Irvine, CA, USA).