WGS of evolved population 5 in changing environments for 224 generations
For each population, genomic DNA was extracted from 107 yeast cells using MasterPureTM Yeast DNA Purification Kit (Lucigen; Cat. No. MPY80200).  Sequencing libraries were constructed using Nextera DNA Flex Library Prep (Illumina; Cat. No. 20018705).  Samples were sequenced using an Illumina HiSeq 4000 with a paired-end (PE) 150 strategy.