RNA-seq of Arabidopsis thaliana:seedlings
Total RNA was extracted from 4-day-old seedlings with embryonic traits using a Hipure Plant RNA Mini Kit (Magen). messenger RNA was isolated according to polyA selection method by oligo(dT) beads and then fragmented by fragmentation buffer firstly. Secondly double-stranded cDNA was synthesized using a cDNA synthesis kit with random hexamer primers. Then the synthesized cDNA was subjected to end-repair, phosphorylation and 'A' base addition according to Illumina's library construction protocol. Sequencing was performed with Illumina HiSeq platform.