GSM3895067: RNASeq_7DaysPI rep1; Brugia malayi; Wolbachia endosymbiont strain TRS of Brugia malayi; RNA-Seq
Batches of nematodes were defrosted and RNA extracted using Trizol reagent. Upper aqueous phases were then aspirated out, bound to, washed and eluted using an RNeasy mini kit (Qiagen). Ribosomal RNA species were then depleted via use of Terminator Exonuclease (Epicentre). Illumina GA-IIx cDNA libraries were then prepared using ScriptSeq v2 library preparation kit (Epicentre), according to manufacturer's instructions.
GEO Accession
GSM3895067