GSM4700922: BAL fluid, Afghanistan_particulate_matter; Mus musculus; RNA-Seq
Fresh BAL cells were obtained by lavage of lungs with PBS, with minimal damage to the tracheas to avoid red blood cell contamination.  Each mouse was lavaged 5 times. To remove mucus in the BAL preparation, cells were resuspended in PBS and passed through 70 µm and then 40 µm pore size cell strainers. Cytospin slides were used to confirm that the cells were in a single cell suspension prior to sequencing.  The viability of cells as evaluated by trypan blue staining was between 60 to 80%. 10x Genomics 3' end kit (v2).
GEO Accession
GSM4700922