ITS Amplicon Sequencing of Wetland Soils
DNA extracted from soils and amplified using ITS1F and ITS2 primers. Amplicon libraries were sequenced using the Illumina MiSeq sequencing platform at a final concentration of 14.3 pM. PhiX was mixed in the pool of amplicon libraries for the sequencing run (expected at 20%). The run was clustered to a density of 681  k/mm2 and the libraries were sequenced using a 300PE MiSeq sequencing kit with the standard Illumina sequencing primers. Image analysis, base calling, and quality assessment were performed on the MiSeq instrument. The resulting sequences were demultiplexed and adapters were removed.