A.rub-bw1
The aboral body wall of one ray of an adult A. rubens was used for RNA isolation (SV Total RNA Isolation System, Promega, Southampton, UK). QuantiNova Reverse Transcription Kit (QIAGEN Ltd, Manchester M15 6SH, UK) was used to produce cDNA and eliminate genomic DNA contamination of the samples. Library preparation (TruSeqv2 kit, Illumina, Little Chesterford, Essex, UK) was performed at the QMUL Genome Centre, London, UK. The sequencing was completed at the BRC Genomics Core Facility at Guys and St Thomas NHS foundation Trust and Kings College London using the Illumina HiSeq 2000 platform.