GSM3351264: OL_Mock3; Mus musculus; RNA-Seq
Brain tissue was dissected, then the nucleus accumbens (or whole brain) was dissociated in papain solution to obtain a single-cell suspension. Samples 1-8 were then processed by Drop-seq. Samples 9-14 were immunostained and oligodendrocytes were purified by FACS, then RNA was isolated using Trizol. For samples 1-8, standard Drop-seq (Nextera) library prep; Read 1 was 25 bp and Read 2 was 75 bp. For samples 9-14, RNA was amplified using the Seqplex RNA Amplification Kit (Sigma), then prepared for sequencing using standard Illumina protocols and sequenced 1x50. single-cell RNAseq; total RNAseq
GEO Accession
GSM3351264