GSM1654726: HEK-BAHD1 WGBS (replicate 2); Homo sapiens; Bisulfite-Seq
Isolated DNA was reduced into 100-300 bp fragments by sonication and treated with DNA-end repair, 3’-dA overhang and ligation of methylated sequencing adapters. Samples underwent bisulfite treatment with the ZYMO EZ DNA Methylation-Gold kit, with 99.6% bisulfite conversion rate. Desalted, size-selected, PCR amplified fragments were size-selected again.
GEO Accession
GSM1654726