Sep_B_CR_R_4
Sequencing at Argonne National Laboratories followed the protocols of Caporaso et al. (2012). PCR amplification of the V4 region of 16S rRNA made use of the forward primer 515F (5- GTGCCAGCMGCCGCGGTAA-3) and the reverse 806R (5-GGACTACHVGGGTWTCTAAT-3). PCR amplicons were quantified by PicoGreen (Invitrogen, Carlsbad, CA) using a plate reader, and amplicons were pooled in equal concentrations into a single tube. This pool was cleaned up using UltraClean PCR Clean-Up Kit (MoBio, Carlsbad, CA) and quantified using the Qubit (Invitrogen, Carlsbad, CA). The pooled samples were sequenced on the Illumina MiSeq platform (Illumina, San Diego, CA) according to the sequencing procedures described in Caporaso et al. (Caporaso et al., 2012).