GSM1482170: Day 3 post NGN induction, biological replicate 2; Homo sapiens; RNA-Seq
The RNA extraction was performed using the mirVana™ miRNA Isolation Kit (AM1560, Ambion) following their protocol. The protocol was interrupted after the first column purification step to obtain the total RNA. The isolated RNA was stored at -80C and submitted to the Broad Institute (Cambridge, MA) where the quality control, library preparation (Illumina dUTP RNA-Seq Library (PolyA method)) and RNA sequencing (Illumina HiSeq (Paired End Run 101 Base)) was performed. Broad Institute (Cambridge, MA) conducted quality control, library preparation (Illumina dUTP RNA-Seq Library (PolyA method)) and RNA sequencing (Illumina HiSeq (Paired End Run 101 Base)) as previously reported.
GEO Accession
GSM1482170