GSM2195101: single-cell RNA-seq M3022-23 treated mrd130; Homo sapiens; RNA-Seq
cDNA synthesis protocol: cells were loaded on the 10-17 μm mRNA-seq IFC (Fluidigm) with ERCC spike-in controls. Cell lysis, reverse transcription and pre-amplification of cDNA was done on-chip using the SMARTer Ultra Low RNA Kit for C1 (Clontech). 0.2-1 ng of pre-amplified cDNA was used as input for tagmentation by the Nextera XT Sample Preparation Kit (Illumina), where amplification was performed for 12-14 PCR cycles. Cells were isolated from the murine bone marrow, enriched by a MACS step and sorted for the population of interest. All steps were performed at 4°C.