Characterisation of microRNAs in the Black flying fox (Pteropus alecto)
SAMPLE PREPARATION:  1. Tissues were harvested from 4 uninfected bats and stored in RNAlater  2. Tissues consisted of: Bone, brain, heart, kidney, large intestine, small intestine, liver, lung, muscle, lymph nodes, salivary gland, skin, spleen, testes  3. RNA was extracted from each tissue using an Ambion mirVana kit  4. Equal quantities of RNA from each tissue were pooled for Illumina short RNA sequencing  SEQUENCING PROTOCOL:  1. Isolate small RNA using a urea-acrylamide gel (~19-23nt)  2. Ligate on 5’ RNA adapter, repurify ligation product on urea-acrylamide gel  3. Ligate on 3’ RNA adapter, repurify ligation product on urea-acrylamide gel  4. Reverse transcribe using specific 3’ adapter primer  5. PCR to amplify library  6. Sequence on single-end Illumina Genome Analyzer flow cell using small RNA sequencing primer. Sequence is from 5’ end, with first base sequence corresponding to 5’ base of small RNA.