GSM4103329: NB1.3; Mus musculus; RNA-Seq
Mouse total RNA was extracted from eight FACS-separated SEZ populations, obtaining 3-4 biological replicates for each of them (total of 28 samples) and subjected to poly(A) selection. The same procedure was used to extract the RNA from the two DFFDA samples FACS-separated from NSC in vitro cultures (4 biological replicates per population). The poly(A) enriched fraction was used to construct libraries for sequencing with the SMARTer Ultra Low RNA Kit for Illumina (Clontech/Takara), using the Dual index Nextera XT v2 Set A Adapters (Illumina). Libraries were sequenced in a NextSeq 500 system (Ilumina).