Illumina HiSeq 2500 sequencing; Inefficient secretion of anti-sigma factor FlgM inhibits bacterial motility at high temperature
Inefficient secretion of anti-sigma factor FlgM inhibits bacterial motility at high temperature
E. coli grown in TB aT 260 rpm at indicated temperature until OD600=0.6 (mid-exponential phase), harvested by centrifugation. GeneMATRIX Universal RNA Purification Kit (including on-column DNAse digestion) TrueSeq RNA library, rRNA depletion