ICP4 ChIPseq of n199 mutant infected cells
MRC cells were infected with wt herpes simplex virus strain KOS or an ICP22 mutant (n199) at an MOI of 10 PFU/cell.  At 6h post infecteion the infected cells were harvested and sujected to ChIP using antibodies to ICP4 (58S) or RNA polII (4H8 or 8WG16).  Illumina libraries were constructed for the ChIPed DNA and 5% of the ChIP input material and subjected to Illumina sequencing.