GSM2195540: 2A3; Triticum aestivum; RNA-Seq
For each sample, the first leaf of two seedlings from different pots were pooled and quickly frozen in liquid nitrogen. Total RNA was extracted using the RNeasy Plant Mini Extraction Kit (Qiagen) and RNA integrity was checked on 1.4% agarose gel and on the Agilent 2100 Bioanalyzer. Poly(A) isolation was performed by the sequencing company, Australian Genome Research Facility. RNA libraries were prepared for sequencing using standard Illumina protocols