GSM3723014: DMSO A; Mycobacterium tuberculosis; RNA-Seq
Bacteria were harvested from the culture by centrifugation, resuspended in Trizol and lysed by bead beating. RNA was extracted by chloroform extraction, followed by isopropanol precipitation, and the extracted RNA was resuspended in water. RNA integrity and concentration was measured with an Agilent Bioanalyzer. RNA libraries were prepared using standard Illumina protocols.