GSM3790183: RNA_LSK_BMT_SKO_rep2; Mus musculus; RNA-Seq
RNA-seq experiments were performed using LSK or CMP cells obtained from WT-, SKO-, RKO-, or DKO-transplanted mice. RNA was extracted using RNeasy Mini Kit (QIAGEN) or NucleoSpin RNA XS (Macherey-Nagel).  Libraries for RNA-seq were prepared using the NEBNext Ultra RNA Library Prep kit for Illumina (New England BioLabs) and were subjected to sequencing using HiSeq 2500 instrument (Illumina) with a standard 125-bp paired-end protocol.