genome sequence of sitophilus oryzae: adult male gonads
Male and female adults of Sitophilus oryzae were used for DNA extraction. Only the gonads were used to minimize DNA contamination from its diet. The sexual organs were obtained from aposymbiotic adults and a DNA extraction protocol specific for Sitophilus was performed. Four different DNA samples were obtained: three from males and one from females. Each sample was made up by the genomic DNA from 20 individuals. The PacBio library was sequenced on 7 SMRT cells were sequenced using P6-C4 chemistry generating reads with an average size of 5,900 bp.