GSM4153486: epe1KO isolate D H3K4me3; Schizosaccharomyces pombe; ChIP-Seq
ChIP DNA was extracted using a bead beater and 0.5mm zirconia beads. Lysates were clarfied following shearing by sonication and then DNA/histone complexes were immunoprecipitated with antibody. Libraries were constructed first with end repair via the Fast End Repair Kit (Thermo) then A-tailed using Klenow fragment (NEB). Illumina adaptors were ligated using Rapid T4 DNA Ligase (Enzymatics), followed by PCR amplification for 15 cycles, and size selection between 200-500bp by bead purification and gel extraction.