GSM5221201: 12h-6; Rattus norvegicus; RNA-Seq
Retinas were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. the integrity of total RNA was determined with an Agilent 2100 Nano 6000 Assay kit on an Agilent 2100 Bioanalyzer system. Samples were submitted to Illumina for library preparation using a NEBNext Ultra RNA Library Prep Kit and fluorometer  to build and preliminarily quantify the library.pair-end 150-bp sequencing, on the HiSeq-2500 platform (Illumina).