GSM4155073: GLUT1KO.6; Mus musculus; RNA-Seq
RNA from the CD45 negative fractions was extracted using TRIzol (15596018, ThermoFisher Scientific). TruSeq stranded mRNA kit (Illumina) was used to prepare sequencing libraries, starting from 500 ng total RNA. We performed a TruSeq stranded mRNA LT prep, starting from 375 ng of RNA, according to Illumina protocol 15031047 Rev. E (internal exp code LPX170).