RNA-Seq of Pisum sativum: immature seeds, water-stressed for 9 days under limiting sulfur conditions, replicate 1
RNA-seq libraries were prepared using the Illumina TruSeq Stranded mRNA sample prep kit according to the manufacturer's instructions (Illumina, San Diego, CA). Briefly, mRNA were selected using poly-T beads, fragmented to generate double-stranded cDNA and ligated to adapter. Eleven cycles of PCR were applied to amplify libraries. Library quality was assessed using a Fragment Analyser (Agilent Technologies, Courtaboeuf, France) and libraries were quantified by QPCR using the Kapa Library Quantification Kit (Roche, Meylan, France)