GSM1357041: E2a_MO_rep1_RNASeq_ATCACG; Xenopus (Silurana) tropicalis; RNA-Seq
400ng of mRNA was used for library preparation following methods published in Tan et al. Genome Research 2013. Total RNA was isolated by homogenizing embryos in SDS/PK buffer for 1 hr, followed by phenol/chloroform extraction and ethanol/NH4OAc precipitation.  mRNA was isolated from total RNA using Oligo-DT Dynabeads (Invitrogen).