GSM4704845: U937 lot2 PMA; Homo sapiens; RNA-Seq
Cells were washed by PBS(-) and peletted by centrifugation. Total RNA were extracted by RNeasy Plus Mini Kit (QIAGEN) and polyA(+) RNA were concentrated by Dynabeas mRNA DIRECT Micro kit (Thermo fisher scientific). Whole transcriptome library was prepared by Ion Total RNA-Seq Kit for the AB Library builder System (Thermo fisher Scientific) and whole transcriptome library was amplified and barcoded by IonXpress RNA-Seq Barcode 1-16 Kit (Thermo Fisher Scientific).