GSM4154606: R61_rep1_2_3_4 [R6/1]; Mus musculus; ChIP-Seq
Crosslinked tissues were fragmented to the size range of 100–500 bp using a Bioruptor (Bioruptor Next Gen; Diagenode). Antibodies that specifically recognizes H3K27 acetylation (Abcam, ab4729) and Elk-1 protein (Santa Cruz, sc-355X) along with nonspecific rabbit IgG (Millipore, 17-614) were incubated with beads for 6 h before incubating with sonicated chromatin overnight. Immunoprecipitated DNA and nonspecific IgG-bound DNA were prepared for high-throughput sequencing according to Illumina's instructions and sequenced on an Illumina platform following the manufacturer's standard protocol.