GSM4154640: 10631483_S9; Homo sapiens; RNA-Seq
Total RNA was isolated from PRAT-SVF using RNeasy mini kits (QIAGEN Inc., Valencia, CA, USA) including a DNase I digestion step removing genomic DNA. Generation of libraries was performed using PerklinElmer technologies with the NEXTflex qRNA-Seq kit v2 after total RNA enrichment by NEXTflex Poly(A) beads following manufacturer's recommendations (PerkinElmer).  RNA-seq library were sequenced on Illumina Nextseq sequencer.