GSM3289915: c2 UAS-tkvRNAi/+; Drosophila melanogaster; RNA-Seq
Total RNA was extracted by Trizol® Reagent (Invitrogen, USA), and followed by RNeasy Mini Kit (Qiagen, Germany). RNA purified was quantified at OD260nm by using a ND-1000 spectrophotometer (Nanodrop Technology, USA) and qualitated by using a Bioanalyzer 2100 (Agilent Technology, USA) with RNA 6000 nano labchip kit (Agilent Technologies, USA). Library construction for all samples was accomplished with Agilent's SureSelect Strand Specific RNA library Preparation Kit for 75SE (Single-End) sequencing on Illumina platform.