GSM5221808: Apoe_minus_minus_M_plus_2; Mus musculus; RNA-Seq
RNA was extracted using the RNeasy (Qiagen) kit following manufacturer's guidelines Libraries were prepared from polyA-enriched mRNA using the universal low-input kit (SMARTer #634940), validated using Agilent TapeStation (Agilent Technologies, Palo Alto, CA, USA), and quantified by using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA) as well as by quantitative PCR (KAPA Biosystem, Wilmington, MA, USA).