Heliopora coerulea transcriptome, 28C, 3week, encrusting colony 2
RNA from coral fragments was extracted using Trizol (Invitrogen) following the manufacturers protocol. RNA samples were sent to the Beijing Genomic Institute (BGI), Hong Kong, for preparation of barcoded libraries using the Illumina TruSeq RNA Sample Prep Kit V2 protocol. The mRNA-enriched libraries were sequenced on the Illumina HiSeq 2000 platform with all cDNA libraries pooled in a single lane to produce 100-bp paired-end reads.