Illumina HiSeq 2000 sequencing; Profiling of total and AGO1-associated small RNAs in Col-0 wild type, rns2-2 and rns2-3 flowers
Profiling of total and AGO1-associated small RNAs in Col-0 wild type, rns2-2 and rns2-3 flowers
Flowers were harvested, immediately frozen in liquid nitrogen and ground with metal beads. Seeds were surface-sterilized by immersion in 70% ethanol for 2 min followed by incubation in 1.5% hypochlorite 0.05% Tween 20 for 10 min and immediately rinsed with water, before being sown in soil containing 4% perlite and 4% vermiculite. Plants were grown in growth chambers (Percival) with a 16-h photoperiod (150 µmol m−2 s−1), 21°C/16°C day–night temperatures, and 70% relative humidity. Trizol extraction NEBNext Small RNA Library Prep Set (Multiplex)