16S rRNA of archaea: bottom waters in the methane seeps at SCS
The Archaea 16S rRNA gene V4 region was amplified with the forward primer Uni519F 5-CAGYMGCCRCGGKAAHACC -3 and the reverse primer Arch806R 5-GGACTACNSGGGTMTCTAAT-3.  16S rRNA  sequencing library construction were conducted according to the manufacturers protocols of NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, USA).