RNA-seq of rat liver, kidney, skeletal muscle and hippocampus samples
The RNA libraries were prepared using the Illumina TruSeq Stranded Total RNA Sample Preparation protocol with the Ribo-Zero Gold Kit and sequenced using the HiSeq4000 platforms following the manufacturers protocol. Samples were sequenced in paired-end mode to a length of 2x76bp base-pairs. Images from the instrument were processed using the manufacturers software to generate FASTQ sequence files. Read quality was assessed by running FastQC on the FASTQ files. Sequencing reads showed excellent quality, with a mean Phred score higher than 30 for all base positions.