GSM5165298: shPRMT5_2; Homo sapiens; RNA-Seq
JHH-7 cells were collected, flash frozen in liquid Nitrogen, and RNA was isolated using RNeasy Plus Mini Kit (Qiagen, Germantown, MD, #74136). Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols