Gascroc_Cre_postTAM_RNA-seq_rep2
Ribo-depleted RNA was fragmented and the first strand of cDNA was synthesized using ProtoScript II Reverse Transcriptase. Then the second strand was synthesized, and the cDNA was ligated with sequencing adaptors. Libraries were amplified by PCR using Index primers. The quality and quantity of the RNA-seq libraries were monitored by library quantification kit (Kapa Biosystems) and Bioanalyzer (Agilent) for paired-end Illumina Hiseq 4000.