NextSeq 500 paired end sequencing; Whole-genome sequencing of polyamine over-producing yeast strains
Whole-genome sequencing of polyamine over-producing yeast strains
Clones of strains were cultivated overnight in 15â€¯mL of Delft medium added with 40 mg/l uracil up to an OD600 around two. The Blood & Cell Culture DNA Mini Kit (Qiagen) was used to extract genomic DNA from the overnight yeast culture using the protocol recommended by the manufacturer. DNA libraries were prepared using a Kapa Hyper Prep Library Prep Kit (Roche Molecular Systems).