3' mRNA-seq of 96 multiplexed individuals of Oryza sativa: vegetative stage leaf blades
Total RNA extracted using RNeasy Plant Mini Kits (Qiagen, USA), and processed in batches of 96 multiplexed, barcoded individuals using a liquid automation-based 3 mRNA-seq quantification approach (https://www.protocols.io/view/barcoded-plate-based-single-cell-rna-seq-nkgdctw). The data analysis pipeline is described here: https://github.com/broadinstitute/Drop-seq/releases and further barcode metadata is available here: https://zenodo.org/record/3533431