Soil_proradix_p12.5
Microbial community structure was characterized using high-throughput sequencing of the  V4 variable region of the microbial small subunit (SSU or 16S) ribosomal RNA (rRNA) and rRNA gene amplicons. A two-step PCR protocol, employing the primers CS1_515F (ACACTGACGACATGGTTCTACAGTGCCAGCMGCCGCGGTAA) and CS2_806R (TACGGTAGCAGAGACTTGGTCTGGACTACHVGGGTWTCTAAT), was used to generate sequencer-ready libraries. Barcoded libraries were pooled and sequenced on an Illumina MiSeq, employing V2 chemistry, at the University of Illinois at Chicago Sequencing Core (UICSQC).