GSM2800758: 4C2_d5_2; Mus musculus; RNA-Seq
Day 3 and day 5 EBs were harvested and total RNA was isolated using an RNeasy mini plus kit (Qiagen, 74134) following the manufacturer’s instructions. The libraries were prepared using the Illumina TruSeq stranded total RNA kit according to the manufacturer’s protocol, except that the PCR amplification was reduced to 8 cycles.