GSM4704861: U87MG TP53 KO c4.8 rep 1; Homo sapiens; RNA-Seq
Total RNA was extracted using RNeasy Mini Kit (Qiagen). CAGE libraries were prepared following the nAnT-iCAGE protocol (Murata et al, 2014). 5 μg of total RNA was reverse transcribed, and the resulting cDNA was biotinylated and cap trapped to capture their 5' ends. Sequencing was performed on illumina HiSeq 2500 (50 nt, single read).