RNASeq of Vitis cinerea B9: Phomopsis-inoculated leaves 0 dpi
Leaves of V. cinerea B9 were spray-inoculated using either a D. ampelina suspension isolated from field infected vines (I), or sterile water (N). One leaf sample for each inoculation treatment was collected from three replicate vines (biological replicates) both before (T0, 3rd leaf) and 48 hours post inoculation (hpi) (T2, 4th leaf),  immediately stored in liquid nitrogen, and transferred to the laboratory for RNA extraction. Barcoded, strand-specific, mRNA multiplexed libraries were prepared as previously described (Zhong et al. 2011).