GSM4767056: SHAM4; Mus musculus; RNA-Seq
FACS sorted cardiac endothelial cells were homogenized in QIAshredder (#79654, Qiagen) and the RNA was purified using RNeasy Plus Micro Kit (#74034, Qiagen) according to the manufacturer's instruction cDNA library was synthesized using SMARTer Stranded Total RNA-Seq Kit V2 – Pico Input Mammalian (Takara Bio, USA) kit according to the manufacturer's instructions. The library quality was determined using bioanalyzer, and sequenced using illumina NextSeq 550 System with the following specifications: 1 X 75bp, 50M single end reads were sequenced using NextSeq 500/550 High-Output v2.5 kit.