GSM4396362: UK6_4; Tetranychus urticae; RNA-Seq
Total RNA was extracted from 100-120 adult female mites of each T. urticae population using the RNeasy Plus mini kit (Qiagen, Belgium) with four-fold biological replication for each population. The quality and quantity of the total RNA were analyzed by a DeNovix DS-11 spectrophotometer (DeNovix, USA) and by running an aliquot on a 1% agarose gel. Illumina libraries were constructed with the TruSeq Stranded mRNA Library Preparation Kit with polyA selection (Illumina, USA). For RO1, IT2, IT3 and ES1, RNAseq libraries were sequenced on an Illumina HiSeq 2500 to generate strand-specific paired reads of 2 x 125 bp (library construction and sequencing were performed at the Genomics Core Facility of the University of Utah, Utah, USA) while for UK6, RNAseq libraries were sequenced on an Illumina HiSeq 3000 to generate strand-specific paired reads of 2 x 150 bp (NXTGNT sequencing platform, Ghent University, Ghent, Belgium).