GSM2533290: GFP_rep1; Mus musculus; RNA-Seq
Differentiated NPCs plated in T25 flasks were lysed in 400 µl/flask of RLT buffer (Qiagen RNeasy Mini kit). Total RNA was purified out using Qiagen RNeasy mini kit and eluted in 20 µl of RNase-free water. RNA libraries were prepared for sequencing using standard Illumina protocols.