GSM2198550: B6-4_CTL; Mus musculus; RNA-Seq
Cells were washed once with PBS and lysed in 2mL of Trizol.  Prior to RNA extraction, an equal amount of ERCC spike-in control RNA was added to each sample, following the manufacturers specifications.  RNA was extracted using the Qiagen RNeasy Mini Kit with on-column DNAse digestion. RNA-Seq libraries were prepared with Beijing Genomics Institute (BGI)'s standard library preparation protocols.