GSM2532957: No_stimulation_mouse_A; Mus musculus; RNA-Seq
Total RNA was isolated using an RNeasy Mini-kit (Qiagen, Valencia, CA) according to the manufacturer's protocol. mRNA was purified from one microgram of total RNA with the use of Ambion Dynabeads® mRNA DIRECT™ Micro Purification Kit Purified mRNA was fragmented, adapters ligated, reverse transcribed to make cDNA, and cDNA amplified with Ion Total RNA-Seq Kit v2, Ion Torrent™, per manufacturer’s recommendations. Samples were individually barcoded using Ion Xpress™ RNA-Seq Barcode 1-16 Kit, Ion Torrent™, before their cDNA was amplified. RNAseq libraries were assessed for concentration and length using Invitrogens Qubit® dsDNA HS Assay Kit (Carlsbad, CA) and Agilent DNA 1000 Kit respectively