GSM2874034: KLF4_FL_ChIP-seq; Mus musculus; ChIP-Seq
Mesenteric arcades were isolated from SMC eYFP+/+ Klf4WT/WT and SMC eYFP+/+ Klf4Δ/Δ mice and snap-frozen in liquid nitrogen. Frozen tissues were ground in a mortar and pestle and the stromal vascular fractions were isolated. Cells were fixed with 1% paraformaldehyde for 10 minutes at room temperature. Cross-linked chromatin was then sheared by sonication into fragments 200-600 base pairs in size. The chromatin fragments were then immunoprecipitated with anti-Klf4 (Sanda Cruz sc20691, 2 µg) antibody complexed with magnetic bead-coupled protein G Library was performed following TruSeq DNA Sample Guide protocol by Illumina