MiSeq of meiotic gene PCR amplicons from Arabidopsis arenosa
Libraries were constructed using 1ng of input DNA in a Low Input, Transposase Enabled (LITE) pipeline developed at the Earlham Institute and based on the Illumina Nextera kits. Each library was constructed using unique 9 bp dual index combinations allowing samples to be multiplexed. Pooled libraries were size selected between 600 and 750bp on a Blue Pippin (Sage Scientific) 1.5% Cassette and then sequenced with a 2x250bp read metric on an Illumina MiSeq sequencer.