GSM3790159: Hi-C_HSPC_BMT_WT_rep1; Mus musculus; Hi-C
Hi-C experiments were performed using HSPCs obtained from WT-, SKO-, RKO-, or DKO-transplanted mice. Briefly, two million c-Kit+ HSPCs of WT/SKO/RKO/DKO-transplanted mice were crosslinked with 1% formaldehyde for 10 min at room temperature.  Cells were permeabilized and chromatin was digested with MboI restriction enzyme, and the ends of restriction fragments were labeled with biotinylated nucleotides and ligated.  After crosslink reversal, DNA was purified and sheared with Covaris M220 (Covaris).  Then point ligation junctions were pulled down with streptavidin beads.  Then libraries were constructed with Nextera Mate Pair Sample Preparation Kit (Illumina) according to the manufacturer's protocol, and subject to sequencing using NovaSeq (Illumina).