GSM1205151: FB.BS; Tuber melanosporum; Bisulfite-Seq
Genomic DNA from FLM, FB and ECM was extracted by grinding in liquid nitrogen followed by DNeasy Plant Mini kit (QIAGEN) purification After an additional phenol:chloroform extraction, gDNA was sheared by sonication to generate DNA fragments in the 150-300 bp size range. Bisulfite treatment and library preparation were carried out as described (Feng et al. 2011), except that the EpiTect kit (QIAGEN) was utilized for bisulfite treatment. Two consecutive rounds of conversion were performed for a total of 10 hours. The resulting libraries were sequenced with  Illumina sequencing technology (Illumna Hiseq 2000 sequencer).