GSM2536491: Raptor fl/fl Rep1; Mus musculus; RNA-Seq
Bone marrow cells were isolated and stained with antibodies to lineage panel, c-kit, Sca-1, CD127, FcgRII/III, CD34, Ly6C, CD115, and CD135.  GMP (10,000 cells; kit+ Sca- CD127- FcgRII/III+, CD34-low, Ly6C-low, CD135-) were sorted directly into RLT buffer (Qiagen).  Total RNA was isolated by Qiagen RNAeasy Micro kit.  For ER-Hoxb8 cells, cells were pelleted and directly resuspended in RLT buffer for RNA isolation using Qiagen RNAeasy Micro Kit. Illumina SmartSeq2 low-input RNA-seq