GSM4391910: ChIP-Seq SETD2_rescue_H3K4me1_rep1; Homo sapiens; ChIP-Seq
2 x 10^6 JHRCC12 cells were cross-linked with 1% paraformaldehyde for 10 min at room temperature and quenched by glycine. Cells were washed with cold PBS, pelleted by centrifugation, and lysed. After sonication, samples were spun down and incubated with 1 μg primary antibody for each ChIP experiment at 4 °C overnight. Magnetic beads (Thermo Fisher Scientific) were added the next day and incubated at 4 °C for 2h. Samples were then washed and histone complexes were eluted. The eluted samples were treated with RNase A, proteinase K, reversed crosslink, and purified with Qiagen PCR purification kit. Library preparation were performed by the Integrated Genomics Operation Core Facility at MSKCC.