GSM3273385: uvr8-2-UV0-R1; Arabidopsis thaliana; RNA-Seq
Each biological sample collected consisted of five pooled rosettes frozen in liquid nitrogen and stored at –80°C. The rosette leaves from each pooled sample were ground with mortar and pestle in liquid nitrogen. An aliquote from the powdered sample was separated for gene expression (RNA-seq and qPCR). RNA was extracted with with GeneJet Plant RNA purification Mini Kit (Thermo Scientific). RNA quality and concentration were measured using Agilent 2100 Bioanalyzer and NanoDrop. RNA-seq library preparation and sequencing was performed at the Institute of Biotechnology, University of Helsinki using two pooled biological replicates. Each replicate consisted of RNA from two different experimental blocks in the field. Libraries were prepared using 1µg total RNA following instructions from Illumina TruSeq Stranded mRNA Library Prep Kit.