MEF_ICE_postTAM_RRBS_rep2
MspI digested DNA samples were end repaired and A-tailed, then TruSeq Nano DNA LT adaptors were ligated. After clean-up, purified DNA was quantified by qPCR using the Kapa Complete Universal Kit. The libraries underwent bisulfite conversion using the EZ DNA Methylation-Gold Kit and were amplified by PCR. After clean-up, libraries were spiked with 10% phiX and sequenced.