GSM3664983: ChIP-seq control rep2; Homo sapiens; ChIP-Seq
Lysates were clarified from crosslinked and sonicated chromatin. Antibody was used to purify the protein interacted DNA. For HiChIP, crosslinked chromatin were digested with HindIII and ligated before sonication and antibody enrichment. DNA sequencing library preparation was conducted using NEBNext Ultra™ DNA Library Prep Kit for Illumina (NEB) following the manufacturer's instructions.