GSM4932289: Unactivated CD4+ 6 hrs_Donor 3; Homo sapiens; RNA-Seq
Cells were lysed in RLT Plus Buffer with the addition of beta-mercaptoethanol according to the instructions provided by the manufacturer. Homogenization and disruption was made using syringe and needle. Lysates were stored at -80 degrees until extraction. RNA was extracted using the Allprep DNA/RNA mini kit from Qiagen. RNA was eluted with 30 µl of RNase-free water. Libraries were constructed with TruSeq Stranded mRNA (Illumina, San Diego, CA, USA)