RNA-Seq of Trichopoda  pennipes
Two female specimens were collected by hand (USA: Ohio: Hocking Co., Keifel Rd. 17-July-2012. J.O. Stireman III) and placed live in a collecting tube. Thoraxes from live specimens were separated and crushed in RNAlater and stored at 4C. RNA was isolated using a trizol/Chloroform extraction and purified using Qiagen RNeasy spin columns following manufacturers protocol: Purification of Total RNA from Animal Tissues (Qiagen 2006).mRNA samples were prepared using NEB RNASeq reagents utilized with an lab specific protocol (HudsonAlpha). Sequencing was conducted with a TruSeq PE v3 Cluster Kit and TruSeq PE v3 SBS reagents.