GSM3408137: Sciatic nerve, 9 days post injury, (myelin removal beads); Mus musculus; RNA-Seq
Cells were extracted from the sciatic nerves of adult CD1 mice. Nerves were dissociated with collagenase and trypsin and cells were either FACS sorted or processed through myelin removal beads prior to collection of single-cell transcriptomes as described by Macosko et al., 2015, Cell. Injured sciatic nerve samples were isolated from the distal segment 9 days following nerve resection. Standard Illumina libraries were generated as described by Macosko et al., 2015, Cell.