Oxford Nanopore MinION WGS of Enterobacter kobei
ONT sequencing libraries were prepared by multiplexing DNA  extracts from four isolates per flowcell using the SQK-LSK108 and EXP-NBD103  kits according to the manufacturer's protocol with the following amendments:  input DNA (1.5g) was not fragmented, 2ml Eppendorf DNA LoBind tubes  (Eppendorf, Hamburg, Germany) were used, all reactions were purified using  0.4x Agencourt AMPure XP beads, incubation time with Agencourt AMPure XP  beads was doubled, elution volumes were reduced to the minimum required for  the subsequent step, and elution was heated to 37C. Libraries were loaded  onto flow cell versions FLO-MIN106 R9.4 SpotON and sequenced for 48 hours.