GSM2195795: Delta_set1 T= 60 min; Saccharomyces cerevisiae; RNA-Seq
Total RNA was extracted from snap frozen cell pellets Trizol extraction. 1 microgram of this was input into the library preperation. PAT-seq (Harrison et al 2015). Cluster Generation: 9pM of libraries per lane using Illumina c-bot. Illumina protocol 15006165 Rev J, July 2012. Sequencing chemistry: 1 x 150bp sequencing using Illumina protocol 15035788 Rev A, Oct 2012