GSM1201488: Restricted Anovulatory rep4; Bos taurus; RNA-Seq
Total RNA was extracted from hypothalamus tissue by homogenisation in Trizol. RNA was then DNAse treated and purified using Qiagen RNeasy spin columns. Standard Illumina single read mRNA-seq libraries were constructed from cDNA which was made from mRNA which had been purified from total RNA by two rounds of polyA tail selection using oligo dT beads.