GSM3896166: M21; Mus musculus; RNA-Seq
hindlimb muscles were washed with PBS, minced, and digested in  Ham's F10 Nutrient mix(Gibco) containing type II collagenase (2 mg/ml), dispase II(2.4U/ml), CaCl2(2.5mM,Sigma) for 1 hr at 37°C.Digested tissue was successively filtered through 70-μm and 40-μm cell strainers (BD Biosciences) and then centrifuged with 500g for 5min. The cell pellet was resuspended with PBS. Tomato+ cells were sorted by Bio-Rad S3e and then single cells were picked with fluorescence microscope. Libraries were prepared according to Nextera® XT DNA Library Prep Kit for Illumina  (Cat# FC-131-1096)