GSM1565577: BMAL1-CLOCK-ChIPed-control; Homo sapiens; OTHER
Then cells were collected after 24 hours incubation followed by the ChIP experiment which was performed as described in previous studies. (Dahl and Collas, 2008) Libraries were constructed with NEBNext DNA Library Prep Master Mix Set for Illumina (NEB).  After end repair and dA-tailing, methylated adaptors (NEBNext Multiplex Oligos for Illumina) were applied to ligate with DNA followed by bisulfite conversion using EZ DNA Methylation Kit (Zymo research).  Then DNA was recovered using AMPure XP beads (Beckman Coulter) after PCR amplification and sequenced by Hiseq2000 (Illumina).