:  16S rRNA amplicon sequencing of mus musculus: fecal
Library construction and sequencing was performed in a 96-well multiplexed format by the University of Missouri DNA Core.  In brief, dual-indexed universal primers F515/R806 , flanked by Illumina adaptor sequences, were used to construct amplicons of the V4 hypervariable regions of 16s rRNA gene. Amplicon libraries were pooled and sequenced on the Illumina MiSeq platform and the V2 chemistry.