Bacterial SSU rRNA gene survey of clay microcosms using Illumina-Miseq platform
Amplicon libraries were prepared by PCR-amplification of the V3 V4 regions of bacterial 16S rDNA using the primers Bakt_341F and Bakt_805R (Herlemann et al., 2011)  with 5  overhangs complementary to priming sequences for Nextera XT-indexed adapters. Obtained libraries were normalized to 4 nM and pooled prior to sequencing and further processing. Libraries (6 pM final concentration) were sequenced using MiSeq Reagent Kit V3 (600 cycles) on the MiSeq System.