GSM3580953: Kasumi1_untreated_RNA-seq; Homo sapiens; RNA-Seq
Total RNA from the cells was isolated using Trizol reagent (ThermoFisher) according to the manufacturer's instructions. The quality of total RNAs was evaluated using an Agilent Tapestation, and RNA Integrity Numbers (RIN) ranged from 9.2 to 10.0 were accepted. Libraries were generated using Illumina's TruSeq Stranded mRNA Sample Prep Kit following manufacturer's instructions. RNA libraries were sequenced on an Illumina HiSeq4000. RNA libraries were prepared for sequencing using standard Illumina protocols