Illumina HiSeq 2500 paired end sequencing; ChIP-seq for histone H3K27Ac on mouse first branchial arch at E11.5
ChIP-seq for histone H3K27Ac on mouse first branchial arch at E11.5
The preparation of the material was as described in Kutejova et al., 2008. First branchial arches were dissected (the whole tissue), crosslinked and frozen in order to pool enough for ChIP. Kutejova E, Engist B, Self M, Oliver G, Kirilenko P, Bobola N. Six2 functions redundantly immediately downstream of Hoxa2. Development. 2008. 135(8):1463-70. PMID: 18321982. MinElute PCR purification kit (Qiagen) ChIP-seq was performed as described in Donaldson et al., 2012, using 20 ug of H3K27Ac antibody (Abcam, ab4729). Donaldson I, Amin S, Hensman J, Kutejova E, Rattray M, Lawrence N, Hayes A, Ward C and Bobola N. Genome-wide occupancy links Hoxa2 to Wnt-beta-catenin signaling in mouse embryonic development. Nucleic Acids Res. 2012. 40(9):3990-4001. 70 to 100 arches were used for each sample. Library preparation was performed using the TruSeq ChIP Sample Preparation kit (Illumina).