GSM2973391: Ishi_DMSO_ATAC_Seq; Homo sapiens; ATAC-seq
Treated cells were trypsinized and purified by centrifugation before being place in nuclei isolation buffer (lysis buffer) containing 0.1% IGEPAL. Nuclei were then counted and 250,000 cells were used in ATAC-seq library preparation Libraries were constructed as described in ATAC-seq: A Method for Assaying Chromatin Accessibility Genome-Wide by Buenrostro et al.  2015 Curr Proc Mol Biol