NextSeq 500 paired end sequencing; RNA-seq of human melanoma cancer cell line A375 treated with BET inhibitor JQ1 against untreated control
RNA-seq of human melanoma cancer cell line A375 treated with BET inhibitor JQ1 against untreated control
A375 cells were grown as monolayer. At 70-80% of confluence, cells were treated with DMSO and JQ1. After 6 hours, treated and control cells were trypsinized and collected in complete medium. Cells were then washed twice in PBS 1x and pellet resuspended in Trizol for nuclear acid extraction. RNA was extracted with Trizol (following manufacturerâ€™s procedures). Genomic DNA was eliminated using the On-Column DNase I Digestion kit. Library were prepared following Truseq stranded mRNA kit (Illumina) instructions.