GSM1845111: piwi_sh_CAPseq; Drosophila melanogaster; OTHER
Cap-seq was performed according to Nachaev et al, Science, 2010 and Gu et al, Cell, 2013 Purified Capped RNA fragments were ligated with 3' and 5' linkers. 1st strand cDNA synthesis was performed using SuperScript III (Invitrogen) and the library was amplified using the Kapa HiFi Library Amp real time Kit (Peqlab) with Illumina TruSeq compatible primers.