16s rRNA amplicon of metagenome: Creek
V1-V2 region of 16s rRNA amplified using NebNext High Fidelity Mastermix and universal primers 27F & 357R . DNA extracted using Power Soil DNA isolation kit. PCR purified with Nucleospin Gel and PCR Clean-up kit. MiSeq was run using Reagent Kit v3 for 600 cycles.