GSM3273321: WT13_OSVZ; Macaca mulatta; RNA-Seq
The fetal monkey brain tissues were partitioned into several sections, after that, the specimens were embedded in OCT and then gradually frozen in an ethnol /dry ice, and stored at −80°C until processing. Frozen tissue was cryosectioned at 30μm onto polyethylene naphthalate (PEN) slides (Leica Microsystems) and place immediately into microslide box on dry ice, and stored at -80°C until use.     Taking the section slides from the -80°C freezer and put them on a blotting paper for 30s, and then transfer the slides into the 95% EtOH for 30s, 75% EtOH for 30s and 50% EtOH for 30s, place the slides on a blotting paper. Add 400ul Nissl stain solution on the slide, and incubate at room temperature for 40s. Proceed through the dehydration series (50%, 75% and 95% EtOH) till the two rounds of 100% EtOH, and leave the slides in the final xylene jar for 5 min. After air-drying in the fume hood for 5 min, proceed immediately with laser capture microdissection (LCM).    Laser microdissection was performed on a Leica LMD6000 (Leica Microsystems). Microdissected tissues including CP, OSVZ, SVZ and VZ were collected directly into QIAzol buffer from the miRNeasy Mini Kit (Qiagen) in a 0.2ml tubes, vortexed, centrifuged, and frozen at −80 °C. RNA was isolated following the manufacturer's directions for the miRNeasy Mini Kit. RNA samples were run on the Agilent 2100 Bioanalyzer (Agilent Technologies). RNA libraries were prepared for sequencing using standard Illumina protocols