GSM3723292: Time restricted feeding 15:00 Participant: 6; Homo sapiens; RNA-Seq
Skeletal muscle (~25-30 mg) tissue RNA extraction was performed with the All Prep kit (Qiagen, Hilden, Germany) according to the manufacturer's directions. RNA quality was assed using the Bioanalyzer instrument (Agilent Technologies) and RNA sequencing libraries were prepared using the Illumina TruSeq Stranded Total RNA with Ribo-Zero Gold protocol (Illumina). Libraries were sequenced on a NextSeq500 instrument (Illumina) with 38- bp paired end