GSM3665177: HTR8/SVneo: Normal input; Homo sapiens; OTHER
Total RNA was isolated from normoxia or hypoxia HTR8 cell lines. Polyadenylated RNA was further enriched from total RNA using a Dynabeads mRNA purification kit (Invitrogen). RNA fragmentation, m6A-IP and library preparation were carried out according to previously published protocols(Wang, X., et al. (2014). "N6-methyladenosine-dependent regulation of messenger RNA stability." Nature 505(7481): 117-120.)