GSM1567593: P(Ci)-P55; Homo sapiens; Bisulfite-Seq
Genomic DNA of tissue samples, WBC or cancer cell lines including the human liver hepatocellular carcinoma cell line (HePG2) and the human cervical cancer cell line (HeLa) were extracted using the DNeasy Blood & Tissue Kit (Qiagen) according to the manufacturer’s protocol.The fully methylated human genomic DNA is purchased (Chemicon/Millipore, CpGenome Universal Methylated DNA, S7821).The plasma cell-free DNAs were extracted using the QIAamp DNA Blood Midi Kit (Qiagen) according to the manufacturer’s protocol. 1) bisulfite treatment of the DNA, 2) a single-tube three-step amplification of methylated CpG islands, 3) gel-purification of the PCR product that serves as the final sequencing library, and 4) high-throughput sequencing using a next-generation sequencer.