GSM5159900: Monocytes - C52; Homo sapiens; RNA-Seq
RNeasy Mini Kit (Qiagen) TruSeq Stranded mRNA kit (Illumina). The purification of PolyA containing mRNA molecules was performed using poly-T oligo attached magnetic beads from 1µg total RNA. Fragmentation using divalent cations under elevated temperature to obtain approximately 300bp pieces, double strand cDNA synthesis and Illumina adapters' ligation and cDNA library amplification by PCR for sequencing. Sequencing is then carried out on paired-end 75 bp of Illumina HiSeq4000