targeted sequencing of homo sapien: adult female
An Agilent Haloplex custom target enrichment kit was used to target 89 know cancer genes related to melanoma and tumors from mucous membranes designed using the Agilent SureDesign software (Agilent Technologies, Santa Clara, CA). Target-enriched libraries were constructed from genomic DNA (3 ľg), following the manufactures instruction (Agilent Technologies). Only library fragments within 175 to 625 bp were considered for the final quantification, normalization, and pooling. All libraries were sequenced on an Illumina HiSeq 2500 (Illumina, San Diego, CA) on rapid mode using paired-end 100 bp reads.