GSM4153740: BxPC-3-GR2; Homo sapiens; RNA-Seq
Total RNA was extracted from gemcitabine-resistant and parental cells. Briefly, cells at 80% of confluence were lysed using TRIzol reagent (Invitrogen) and scraped on ice. The miRNeasy Mini Kit (50) (cat. no. 217004; Qiagen) was used to extract and purify cellular RNA. RNA libraries were prepared for sequencing using standard Illumina protocols