132-16S amplicon:convnetionall tillage
Soil total DNA was extracted from 0.2 gr soil samples using the GeneAll Exgene Soil DNA isolation kit , and PCR amplification of 16S rRNA gene fragments was performed using 515F and 806R primers. Pooled libraries, with a 20% phiX spike-in, were sequenced on an Illumina MiSeq V2 or V3 kits. In all cases, Fluidigm sequencing primers, targeting the CS1 and CS2 linker regions, were used to initiate sequencing. De-multiplexing of reads was performed on instrument. Library preparation and pooling were performed at the University of Illinois at Chicago Sequencing Core (UICSQC.)PCR amplicon was sequenced using Illumina MiSeq .