Crassostres hongkongesis transciptome
To maximize the discovery of genes in C.hongkongensis,a normalized cDNA library from various adult tissues and embryos was constructed.mRNA from each sample group was combined into a single pool and mixed well.Approximately 2.5 μg of the normalized cDNA pool was used for a titration run using one quarter of a plate on the Roche 454 GS FLX sequencer (Roche, Basel, Switzerland), and following 15 μg of cDNA was sequenced by a whole plate run on the same equipment at the University of Illinois.