V7-V9 targeted Summer Season Sample
In order to decipher how selected hypervariable region(s) of 16S rRNA gene influences the amplicon-based diversity studies of complex marine ecosystem, marine sediments were sampled from the southern coastal region of India. Whole community DNA was extracted using PowerSoil DNA Isolation Kit (MoBio Laboratories Inc., USA) following the manufacturer's instructions. Nucleic acids from three independent extractions were combined to prepare representative samples and the quality was measured by picogreen (Invitrogen) using Victor 3 fluorometry. A total of five widely used universal primer pairs spanning hypervariable region(s) V1-V3, V3-V4, V4, V5-V6 and V7-V9 were employed to amplify 16S rRNA gene. The size and quantity of prepared libraries were validated by running on Bioanalyzer 2100 (Agilent Technologies, California, US) and then sequenced on the Illumina MiSeq sequencer using 300-bp paired-end method.