GSM4767246: RNASeq_NDC_iPSCNeuron_rep8; Homo sapiens; RNA-Seq
Total RNA from magnetically purified human NDC (replicates, n = 8), PSEN1M146L (replicates, n = 4), PSEN1H163R (replicates, n = 3) , PSEN1A246E (replicates, n = 3), and PSEN1A431E (replicates, n = 4) hiPSC-derived neurons was prepared using RNeasy Plus Micro Kit (Qiagen Cat. 74034) according to the manufacturer's protocol. On-column DNAse digestion was subsequently performed on total RNA extracts according to the manufacturer's recommendations to remove any genomic contamination (Qiagen Cat. 79254). Libraries were prepared for RNA-Seq using the TruSeq Stranded Total RNA Library prep kit (Illumina, Cat. RS-122-2303) by the Ribo-Zero ribosomal RNA reduction method (Illumina, Cat. MRZG12324). Samples were sequenced at the UC San Diego Institute for Genomics Medicine (IGM) sequencing core on an Illumina HiSeq4000 generating Paired-End, 100bp reads with an average of 25 million reads per sample (Illumina, Cat. FC-410-1001).