GSM4932679: 4-Day7NoTx; Mus musculus; RNA-Seq
Tumors were bluntly resected and cut into small pieces and digested using Tumor Dissociation Kit, mouse (Miltenyi) in gentleMACS™ Dissociator (Miltenyi) according to manufacturer's instruction. After digestion, dead and dying cells were magnetically separated using a Dead Cell Removal Kit (Miltenyi), after which immune cells were magnetically enriched using CD45 (TIL) MicroBeads (Miltenyi). Single cells were partitioned using a Chromium Controller (10x Genomics), and gene expression sequencing libraries were generated using Chromium Single Cell 3′Library & Gel Bead Kit v3 (10x Genomics). The libraries were pooled and sequenced using an Illumina NovaSeq 6000 (Illumina)