GSM4257207: Experiment 1; Rattus norvegicus; RNA-Seq
Trizol extraction of total RNA form the uterien arteries were performed according to the manufacturer's instructions. The rRNA depleted total RNA was reverse transcribed, fragmented, and 3' end repaired, after adding a “dA” base to the 3' end, the Illumina adapter was ligated to the double strand cDNA. The adpter ligated library was then amplified using Illumina's proprietary amplification primers. Completed libraries were qualified on Agilent 2100 Bioanalyzer for concentration, fragment size distribution between 400~600 bp paired end RNA- seq