genome sequence of sitophilus oryzae: adult male gonads
Male and female adults of Sitophilus oryzae were used for DNA extraction. Only the gonads were used to minimize DNA contamination from its diet. The sexual organs were obtained from aposymbiotic adults and a DNA extraction protocol specific for Sitophilus was performed. Four different DNA samples were obtained: three from males and one from females. Each sample was made up by the genomic DNA from 20 individuals. The illumina library was generated with an average fragment size of 200 bp and paired-end sequenced with a read size of 125bp.