CAGE_HSPC
Committed erythroid and myeloid progenitors/precursors (EPP and MPP) were isolated as pools of CD34lowCD36high and CD34-CD13+ populations, after induction for five days in culture in the presence of SCF, IL3 and either Epo or G-CSF, respectively. RNA was extracted from multipotent and lineage-committed progenitors using RNeasy Plus Mini kit (QIAGEN).  CAGE library preparation was performed by DNAFORM Inc. at RIKEN Omics Science Center.