Transcriptome analysis in TMPRSS2 KO mice.
Total RNA was prepared from ~50 mg of RNAlater (LifeTechnologies) treated lung or bronchi using RecoverAll Total Nucleic Acid Isolation kit (Ambion), followed by selection of poly-A mRNA using FastTrack? MAG mRNA Isolation Kit (Invitrogen). RNA strand-specific RNA-seq library was prepared from ~50 ng of the purified poly-A mRNA using ScriptSeq? v2 RNA-Seq Library Preparation Kit (Epicentre). Prepared RNA-seq library was sequenced by Illuimna GAIIx sequencer (Illumina).