RNAseq of enriched active Arabidopsis thaliana telomerase from seedlings: Col-0 WT, stranded library
Telomerase protein complexes from five-day-old wild type (Col ecotype) seedlings wereprecipitated with PEG8000. The pellet wasresuspended andsize fractioned through aSuperose6 Increase 10/300 GL column. Fractions containing the highest telomerase activity wereimmunoprecipitated with anti-AtTERT antibody. The library wereconstructed by theNEBNext Ultra Directional RNA Library Prep Kit and sequenced on an 300x2 Illumina MiSeq platform