Antarctic Mixotrophic Protists
chlorophyll maximum water, no added bacteria, 72 hour incubation, whole DNA; ; PCR amplification of the V9 region of the 18S ribosomal RNA gene using eukaryote universal primers 1391F and 1510R; the forward primer contained 12 different Multiplex IDs; 3 PCR reactions were performed for each sample, and each reaction was purified using AMPure XP beads; the reactions were pooled and sequenced by the University of Pennsylvania DNA Sequencing Facility using the GS Junior Titanium emPCR Lib-L kit.