GSM4154989: Con-8-C_NDME13338; Mus musculus; OTHER
the mouse cecum contents were collected into 1.5 ml sterile microcentrifuge tubes separately, then sent to Novogene Co. Ltd. (Beijing, China) at low temperatures quickly after liquid nitrogen flash freezer. Metagenomic DNA was extracted using standard protocol by Novogene Co. Ltd. There were about three indicators to evaluate DNA samples quality: purity, concentration and the degree of degradation. After quality control (QC) for DNA, 1 μg DNA in each sample was prepared for generating sequence libraries using NEBNext Ultra DNA Library Prep Kit. First, the DNA sample was fractured to a length of approximately 300 base pairs by ultrasonication, then the DNA fragments were amplified by PCR. Finally, the qualified library preparations were sequenced on an Illumina HiSeq2500 platform and pair-ended reads were generated for each sample.