GSM4103370: WT; Tetrahymena thermophila; RNA-Seq
Total RNA of log-phased △SMYD1 and wild-type CU428 cells (negative control) were extracted using Qiashredder (Qiagen, 79654) and RNeasy Kit (Qiagen, 74624) according to protocol provided in TetraFGD (Xiong et al., 2013). Qubit RNA Assay Kit in Qubit 2.0 Flurometer (Life Technologies, CA, USA, Q32852) and RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA, 5067-1511) were used to measure the RNA concentration and integrity, respectively RNA libraries were prepared with the Illumina TruSeq protocol according to the manufacturer's protocol for RNA-seq libraries.