GSM1566001: DC_MTB_rep3 [wtRNA-Seq]; Homo sapiens; RNA-Seq
Total RNA was extracted from the same samples using the miRNeasy kit (Qiagen). RNA quantity was evaluated spectrophotometrically, and the quality was assessed with the Agilent 2100 Bioanalyzer (Agilent Technologies). Only samples with no evidence for RNA degradation (RNA integrity number > 8) were kept for further experiments. For three individuals, we also performed RNA-Seq on the whole transcriptome following ribosomal depletion using the Ribo-Zero Gold depletion and the Illumina Total Stranded RNA Library kits (Illumina).