GSM4932403: Naïve AGS cells - rep2; Homo sapiens; RNA-Seq
Total RNA was extracted from 24 hour H. pylori-infected and uninfected AGS cells, using TRIzol Reagent (Invitrogen) and purified utilizing DNAaseI (Promega) and RNAeasy Mini kit (Qiagen). PolyA-containing transcripts were purified using Dynabeads mRNA DIRECT Micro Kit (Thermo Fisher Scientific). Sequencing libraries were prepared using Ion Total RNA-Seq Kit v2 according to manufacturer's protocol (Thermo Fisher Scientific). Template preparation was implemented on Ion Chef using Ion PI Hi-Q OT2 200 Kit.