GSM3722738: sgRNA-enrichment_5K-sgRNAs_Batch_4_2; Homo sapiens; RNA-Seq
Cells were collected, washed once with PBS. Then the cells were resuspended in PBS containing 0.05% BSA to the desired concentration and immediately load on the 10X Chromium controller for the single-cell RNA-seq preparation. For single-cell library preparation, we follow the standard protocol of 10X Chromium 3' V2 kit. After the cDNA amplification, 20µg of samples were separated for the sgRNA enrichment library preparation.