Whole metagenome sequencing of pig colon digesta: Sample 4, control diet, pig 65
DNA was extracted with a MagAttract PowerMicrobiome DNA/RNA Kit (MO BIO Laboratories Inc., Carlsbad, CA, USA). The extracted nucleic acids were treated with an Rnase cocktail (Rnase A and T1), then washed with an aqueous solution of NaCl and ~70% ethanol prior to library preparation with the Illumina TruSeq, PCR-free DNA Library Prep Kit. All 24 Whole Metagenome Sequencing sample libraries were sequenced together on 2 lanes of an Illumina HiSeq 4000 with a reagent kit for obtaining 2X150, paired end reads.