GSM2810598: PET111:GFP-negative cells 5 days 16 hours rep 2; Arabidopsis thaliana; RNA-Seq
Approximately 1mm of the root tips were collected and protoplasted. Protoplasts were sorted using a MoFlo cell sorter, and GFP-negative cells were collected in a solution of RLT buffer and beta-mercaptoethanol. Total RNA was extracted using the Qiagen RNeasy Micro Kit. Libraries were synthesized and multiplexed using the Illumina TruSeq Stranded mRNA kit