GSM4704964: RNA-seq in A549 cells after control siRNA treatment for double knockdown samples rep1; Homo sapiens; RNA-Seq
For RNA sequencing, RNA was extracted using the Aurum Total RNA Mini Kit (# 7326820, Bio-Rad, CA, USA). cDNA was synthesized using an iScript cDNA Synthesis Kit (# 1708891, Bio-Rad, CA, USA). RNA-seq was performed after siRNA knockdown using libraries created by MedGenome (Foster City, CA), and sequenced using 100bp paired-end reads on an Illumina NovaSeq 6000 by MedGenome (Foster City, CA).