16S rRNA amplicon sequencing of Fermented Maiz
The V4 hypervariable region of the 16S rRNA gene was amplified using primers 515F and 806R. PCR reactions were carried out with Phusion High-Fidelity PCR Master Mix (New England Biolabs, USA). The libraries generated with NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, England) were sequenced using paired-end Illumina sequencing (2250bp) on the HiSeq2500 platform (Illumina, USA).