Klebsiella pneumoniae KPPR1 insertion site sequencing (InSeq) of lung samples
Transposon insertion site fragments were constructed from genomic DNA of Klebsiella pneumoniae KPPR1 mutants cultured from infected lungs, as described in Goodman, A. L., Wu, M., & Gordon, J. I. (2011). Nature Protocols, 6(12), 1969–1980. doi:10.1038/nprot.2011.417