Complete genome sequencing of H. alvei A23BA
Broth culture of isolate was pelleted out and the pellets were resuspended in the cryoperservative of a Microbank™ (Pro-Lab Diagnostics UK, United Kingdom) tube and stored in the tube. Approximately 2x10^9 cells were used for high molecular weight DNA extraction using Nanobind CCB Big DNA Kit (Circulomics, Maryland, USA). DNA was quantified with the Qubit dsDNA HS assay in a Qubit 3.0 (Invitrogen) Eppendof UK Ltd, United Kingdom). Long read genomic DNA libraries were prepared with Oxford Nanopore SQK-RBK004 kit and/or SQK-LSK109 kit with Native Barcoding EXP-NBD104/114 (ONT, United Kingdom) using 400-500ng of HMW DNA. Twelve to twenty-four barcoded samples were pooled together into a single sequencing library and loaded in a FLO-MIN106 (R.9.4 or R.9.4.1) flow cell in a GridION (ONT, United Kingdom).