fecal bacterial 16sRNA v3-v4  of no-fiber diet fed mouse, sample 3
Microbial DNA was extracted from mouse feces using Quick-DNA Fecal/Soil Microbe Microprep Kit (Zymo Research). The V3-V4 hypervariable region of the bacteria 16S rRNA gene was amplified by PCR using tagged 341F and 805R primer and Phusion DNA polymerase.  Multiplexing indices and Illumina sequencing adapters were then added to the amplicons by limited-cycle amplification using the Nextera XT Index Kit (Illumina). The libraries were normalized and pooled, and sequenced by Paired-end sequencing (2300 bp) using the Illumina MiSeq system platform.