GSM1845860: 2,4-18 RNA-seq; Homo sapiens; RNA-Seq
Cells were harvested by adding 700 microliters Trizol lysis buffer to each 6-well immediately following removal of growth medium. Total RNA was purified following the Qiagen miRNeasy protocol followed by DNase treatment of total RNA. Total RNA was treated according to STARSeq roitines (2014), including Agilent Bioanlyzer2100, all RIN values >10, rRNA depletion, cDNA synthesis, concentration and quality control (Qubit/Agilent Bioanalyzer), fragmentation and sizing (Covaris TMS2), sequencing preparation with barcoded adapters for muliplexing and Illumina NextSeq 500 sequencing, paired end read, 150 nt, 100 Mio reads.