GSM4153691: pan_27; Mus musculus; Bisulfite-Seq
Pancreas samples were homogenized by using a TissueLyser II (Qiagen) with the following settings: 4 x 30sec, 30Hz with RNAse/DNAse-free ceramic beads. Tissues were dissolved in RLT lysis buffer of the DNA/RNA Allprep Kit (Qiagen) following the manufacture Extracted DNA was subjected to the reduced representation bisulfite sequencing (RRBS) workflow as described previously (Klughammer et al, 2018). One hundred ng of DNA were digested at 37°C for 12 h with 20 units of MspI and TaqI (New England Biolabs) in e