RNA-Seq of Caerostris darwini: adult female major ampullate gland
Total RNA was extracted from the major ampullate glands of a single adult female by homogenization in TriZol and cleanup using Qiagens RNeasy kit. DNA was removed from RNA with a TURBO DNase kit (Ambion). The total RNA was submitted to the University of Massachusetts Medical School Deep Sequencing Core Laboratory, where cDNA was synthesized using the the Iso-Seq protocol (Pacific Bioscience, Inc; Menlo Park, CA).  The cDNA was fractionated into two size distributions (1) 0-1.2 kb and (2) >1.2 kb.  The larger fraction was used to construct a SMRTBell library, which was sequenced on three SMRTCells of a PacBio RS II instrument using 120-minute movies.