GSM3289904: RELA1h1; Homo sapiens; ChIP-Seq
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. For sequencing, adapters were ligated to the precipitated DNA fragments or the input DNA to construct a sequencing library according to the manufacturer's protocol (Illumina, San Diego, CA).  Adapters with a T overhang were ligated to the DNA fragments and size selected (~200 - 350 bases) on a 4.5% agarose gel. 18-cycles PCR amplification were performed to enrich for fragments with an adaptor on both ends.  These samples were bound to an Illumina single-read Flowcell, followed by cluster generation on the Illumina Cluster Station, and sequencing with Illumina Genome Analyzer (GA-II).