Lates calcarifer transcriptome
Total RNA was extracted from Lates calcarifer, and followed by Ribosomal RNA depletion using the RiboMinus™ Eukaryote Kit (Invitrogen, P/N A10837-08). Total RNA integrity and the rRNA removal efficiency was confirmed by the bioanalyzer analysis. cDNA library preparation was then carried out using the GS FLX Titanium Rapid Library Preparation Kit (Roche, 05608228001). The cDNA library was clonally amplified onto beads by emulsion PCR and then sequenced on a Genome Sequencer FLX Instrument.