RNA-Seq analysis of developing seeds at 10-20 days after flowering of GM rice OSCR11 (Oryza sativa L. ssp. japonica cv. Koshihikari) - replicate 1
Total RNAs were prepared from developing seeds at 10-20 days after flowering of three independent OSCR11 plants with phenol/SDS methods. After DNase I treatment, mRNAs were purified twice with Dynabeads mRNA purification kit (LifeTechnologies). Libraries were generated with Ion total RNA-Seq v2 kit (LifeTechnologies), and sequenced by Ion Torrent PGM (LifeTechnologies) with Ion 318 Chip (LifeTechnologies). BioProject of this experiment was changed from PRJDB1922 to PRJDB1921.