GSM3665189: D711rna_D501rna; Mus musculus; RNA-Seq
epithelium was single-cell digested and FACS sorted for GFP positive and neagative cells (excludin dead cells (DAPI pos), debris (Draq5 neg) and immune cells (CD45 pos)). RNA was extracted using microplus RNeasy kit (Qiagen) and quality checked on a picochip bioanalyzer 10ng of RNA was used for library contruction using the SMARTer stranded total RNAseq kit v2 pico kit (Takara) RNAseq SE-50 on an Illumina HiSEQ 4000