Environment shaped diversity of the aquatic Marine Group I Thaumarchaea along a subtropical estuary
Surface and bottom water samples of 6 sites at 1m depth and 1-2m above sediment respectively. Samples for nucleic acids were prefiltered through 3 mm pore size filters to remove larger organisms and particles and then filtered through 0.22 mm Millipore membranes. Filters were frozen at -20 oC onboard and -80 oC in the laboratory until nucleic acids were extracted. Archaeal universal primer 344F/915R were used to amplify metagenomic DNA.  Barcodes resemble samples as given: barcode P01_0 CAGTGTGT; barcode P01_2 CATCGTAG; barcode P03_0 CATGAGCT; barcode P03_2 CGATGCAT; barcode P07_0 CGTACGAT; barcode P07_2 CGTATACG; barcode C2_0 CTGTAGTG; barcode C2_2 CTGTCTGT; barcode A08_0 CTACGTAG; barcode A08_2 CTAGAGCT; barcode F412_0 CTCACTGT; barcode F412_2 CTCTAGAG;