GSM3408500: snv_IntLo-R2; Homo sapiens; OTHER
We extracted genomic DNA for the sorted populations using blood and cell culture DNA midi kit (Qiagen). We amplified each sublibrary for ~300-fold amplicon coverage, and reactions were performed in 96-well format in 50 μL reactions for each sublibrary proportional to bin size using Kapa HiFi DNA polymerase. Custom libraries were constructed using standard adapters from Illumina and paired-end sequenced with custom sequencing primers. Samples were indexed with i7 indices compatible with Illumina paired-end protocols.