CRISPR_DigDeep_KO_screen: Ms induced pluripotent stem cells, Roadblocks of reprogramming MEF to iPSC, Positive Selection
SR50, DUAL INDEXING, Read 1: custom primer (first 20bp are sgRNA Sequence) index read1: UMI (first 10bp random nucleotides used as unique molecular identifiers) index read2: 6bp sequences for demultiplexing (see supplementary information CRISPR DigDeep)