CBS432xDBVPG6304 Interstrain S. paradoxus hybrid
An interstrain S. paradoxus hybrid yeast strain was produced by replacing one allele of URA3 with HYG in DBVPG6304 diploids and one allele of URA3 with KAN in CBS432 diploids, followed by sporulation, random mating, and selection for dual resistance. Single colony picks of the hybrid strain were grown overnight in YPD medium at 30°C in a C24 Incubator Shaker (New Brunswick Scientific). RNA was immediately isolated from the pellets using the MasterPure™ Yeast RNA Purification Kit (Epicenter). Two µg of RNA from each of the samples was subsequently used to create sequencing libraries using the low-throughput protocol in the TruSeq RNA Sample Preparation Kit v2 (Illumina). And sequenced (single-end 36 bp reads) on a single lane of an Illumina HiSeq 2000 instrument at the Stanford Center for Genomics and Personalized Medicine.