GSM4395552: MCF_7_RNA_siBRD4_Pan_2; Homo sapiens; RNA-Seq
RNA was harvested using miRNeasy Mini Kit (Qiagen #217004) following the manufacturer's instructions. Integrity was verified using the Agilent 2100 Bioanalyzer. All samples had a RIN score of 9 or higher. One microgram of total DNase-treated RNA was prepared by using the TruSeq Stranded Total RNA LT Sample Prep Kit from Illumina (Illumina  #RS-122). Total RNA was depleted of its rRNA and fragmented before strand-specific cDNA synthesis.  cDNA was A-tailed and then ligated with indexed adapters. After adapter ligation, samples were PCR-amplified and purified with AmpureXP beads, then validated again on the Agilent 2100 Bioanalyzer. Samples were quantified by Qubit before being normalized and pooled for sequencing.