GSM2532799: WT_4h_3; Mus musculus; RIP-Seq
HITS-CLIP Cross-linked RNA was ligated to 3' adapter, then purified by SDS-PAGE, transferred to nitrocellulose, and extracted with protease treatrment. RNA was reverse transcribed to cDNA with indexed RT primers in the presence of bromodeoxyuirdine. cDNA wasthen  captured on beads with anti-Br-dU antibody and circularized with CircLigase to position adapter sequences on the 5' end. Libraries were then PCR-amplified using primers with Illumina adapters.  Please see accompanying protocol for details.