GSM5076013: GCN2 KO MEFs_ATF4 ChIP-seq_No Leu; Mus musculus; ChIP-Seq
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with an ATF4-specific antibody. Libraries were prepared at the Genome Quebec Innovation Centre (McGill University) using the TruSeq DNA sample preparation kit according to Illumina's recommendations and sequenced as single 50bp reads on an Illumina HiSeq 2500 sequencer.