GSM3895604: Nerve Macrophage, Aged, Rep 1; Mus musculus; RNA-Seq
All cells were sorted directly into lysis buffer (Ambion), then triturated and vortexed before storage at -80 °C. For RNA extractions, all samples were processed together following manufacturers recommendations (AM1561, Ambion). Total RNA extracts were quantitated using a Qubit Fluorimeter and the Qubit HS assay kit (Invitrogen). All samples were subjected to reverse transcription and Multiple Displacement Amplification (MDA) with REPLI-g SensiPhi DNA Polymerase and oligo-dT primers as per the Qiagen REPLI-g Single Cell RNA Library preparation kit (150073) and the manufacturer's protocol. RNA libraries were prepared for sequencing using standard Illumina protocols