Aphidius ervi genome -  Pea aphid (A.pisum) strain
Long read PacBio (Pacific Biosciences) RS II sequencing was performed from a single DNA extraction of 270 A. ervi females, collected from A. pisum. Genomic DNA was extracted using the Wizard genomic DNA purification kit (Promega) according to manufacturer instructions and was quantified spectrophotometrically using a NanoDrop 2000 (Thermo Scientific). Input DNA was mechanically sheared to an average size distribution of 10Kb (Covaris gTube, Kbiosciences), and the resulting library was size selected on a Blue Pippin Size Selection System (Cat #BLU0001, Sage Science, Beverly, MA, USA) to enrich fragments > 8Kb. Quality and quantity were checked on Bioanalyzer (Agilent Technologies) and Qubit, respectively. Four SMRT RSII cells with P6 chemistry were sequenced at GenoScreen, France.