MEF_ICE_postTAM_RNA-seq_rep2
5 g total RNA was subjected to ribosomal RNA depletion and ribo-depleted RNA was quantified. 20 ng ribo-depleted RNA was fragmented and the first strand of cDNA was synthesized using SuperScript III Reverse Transcriptase. Then the second strand was synthesized, and the cDNA was end-repaired and tailed with dA. The end repaired, dA tailed cDNA libraries were ligated with index sequencing adaptors and amplified by PCR. The quality and quantity of the RNA-seq libraries were monitored by library quantification kit (Kapa Biosystems) and Bioanalyzer (Agilent) for 75 bp, paired-end Illumina NextSeq.