GSM4103180: ko_rep2_input (for gtf2i_comparison); Mus musculus; ChIP-Seq
E13.5 whole brains were dissected and frozen in liquid nitrogen until ChIP experiment was performed. The whole brain was homogenized and crosslinked in formaldehyde. Cells were lysed and nuclei were sonicated in a Covaris to a size of 200-500bp. Chromatin was precleared using Streptavidin coated Protein G beads. 80ul was taken as input control. 400ul of pre-cleared lysate was incubated with 10ug of Gtf2i BD sciences primary antibody or 10ug of Gtf2ird1 Novas primary antibody over night. The IP samples were eluted and de crosslinked at 65 degrees . The input and IP DNA samples were prepared into libraries using the Swift Biosciences Accel 2S plus kit. the libraries were enriched by 13 rounds of PCR. Swift Biosciences Accel 2S plus DNA library kit