GSM5163373: Mouse AP/NTS ad libitum fed repitition 1; Mus musculus; RNA-Seq
an area of the brainstem containing the Area Postrema and Nucleus of the Solitary tract was dissected, and a single nucleus suspension was created using a dounce homogenizer and purified using Fluoresence activated cell sorting (FACs) Library constriction was perfomed using 10X Genomics Chromium Single Cell 3' Reagent kits (Version 3) according to the standardised protocol. Single nucleus RNA-sequencing