GSM1846071: RKO_uv0.0h; Homo sapiens; OTHER
Nuclear RNA was purified using the RNeasy kit (Qiagen) following manufacturer’s protocol. Total RNA was processed by the 3’ READS method as previously described (Nat Methods. 2013 Feb;10(2):133-9.) 3'READS (a recently developed deep sequencing method for analysis of APA isoform expression genome-wide)