GSM4154715: B-0028_VP4 [GG4CR2]; Homo sapiens; OTHER
B lymphoblastoid cell lines (5 x 10^6 cells) were harvested by centrifugation and resuspended in 5 mL PBS with 10% FBS. To cross-link cells, 5 mL 4% formaldehyde was added and samples incubated for 10 min. Cross-linking was quenched by adding 1 M glycine to 125 mM final concentration and cells collected by centrifugation at 300 x g for 10 min at 4°C. 4C-seq assays and data processing were performed as previously reported (Fernández-Miñán et al. 2016)