RNA-seq of Gymnosporangium japonicum teliospores
Branch samples of juniper infected by G. japonicum were collected from the Yat-sen Mausoleum, Nanjing, Jiangsu, China, in March 2016. Batches of 50 mg of mature teliospores were recovered with a needle by gently scratching from beneath the bark of the branch. Three replicate samples were collected in sterile tubes. Teliospores were ground to powder with sterile mortar and pestle in liquid nitrogen. RNA was stored in DEPC-treated water. Tagged cDNA libraries were pooled in equal ratios and used for 100-bp PE sequencing on the Illumina HiSeq2000 platform.