GSM1346281: Smad3-KO-UUO-X547; Mus musculus; RNA-Seq
Kidney tissues were immediately frozen in liquid nitrogen and stored at -80 °C until RNA extraction. Trizol reagent (Life Technologies, Carlsbad, CA, USA) was used to isolate total RNA from the kidney tissues in accordance with the manufacturer's instructions. Then, total RNA samples were used to remove highly abundant ribosomal RNAs. For each sample, RNA samples were fractionated with a denaturing polyacrylamide gel (urea-PAGE). Then RNAs over 150 bp were used for cDNA synthesis. After adenylating 3' end and ligating the adapter, the RNA samples were then used for library construction following the protocol suggested by the manufacturer.