RNA Seq V583 Induced 1
Bacteria were grown to OD600 of 05.-0.6 and 1 mg/ml lysozyme final concentration was added and bacteria were grown for an additional hour at 37 degrees C.  Bacterial cells were harvested by centrifugation, cell pellets were resuspended in trizol reagent and bacteria were lysed in a Beadbeater for 1 minute after the addition of 0.1 mm zirconia beads.  Following centrifugation, clarified lysates were applied to a ZymoResearch DirectZol column for RNA isolation.  Purified RNA was DNAse I-treated followed by removal of ribosomal RNA using the Epicentre RiboZero kit for Gram-positive bacteria.