16S amplicon sequencing of pig intestinal microbiome: Sample 311, duodenum from pig 82
DNA was extracted with a MagAttract PowerMicrobiome DNA/RNA Kit (MO BIO Laboratories Inc., Carlsbad, CA, USA). The V3-V4 region of the 16S rRNA gene was PCR amplified using the primers Pro341F (5'-CCT ACG GGN BGC ASC AG-3') and Pro805R (5'-GAC TAC NVG GGT ATC TAA TCC-3'). 25 cycles of amplification were followed by 8 indexing cycles with the Nextera XT Index Kit v2 (Illumina, San Diego, CA, USA) indexes, completion of the library preparation according to the Illumina protocol for 16S metagenomic sequencing library preparation, and sequencing with the Illumina MiSeq 2X300 Reagent Kit v3.