GSM1204468: FAIRE_Seq_rep2; Homo sapiens; OTHER
For FAIRE, nuclei were extracted after the crosslinking step (1% formaldehyde in PBS) of intact cells, followed by re-suspension in SDS lysis buffer before sonication. FAIRE DNA samples were purified by phenol/chloroform extraction. Input DNA was also purified by phenol/chloroform extraction after the crosslinking was reversed. ChIP assays were performed in MDAMB231 as previously described (Jia et al, 2009). Libraries were prepared according to Illumina's instructions (refer to http://epigenome.usc.edu/services/nextgen/making_libraries.html)