GSM1570171: Bmi1KO_M6; Mus musculus; RNA-Seq
Twelve 17-weeks post induction per genotype were killed by cervical decapitation and heart (left ventricle) tissues were dissected and immediately frozen in liquid nitrogen. Total RNA was extracted with QIAzol Lysis Reagent and then purified on RNeasy spin columns (Qiagen). RNA integrity (RNA Integrity Score ≥ 7.9) and quantity were determined on the Agilent 2100 Bioanalyzer. RNA samples from different animals were mixed in equal proportion at four samples per pool, and three pools per genotype were analyzed in the Illumina Genome Analyzer IIx. RNA libraries were prepared for sequencing using standard Illumina protocols