PacBio sequencing of Salmonella enterica serovar Heidelberg from Brazil
Genomic DNA was extracted using the QIAamp DNA Mini Kit (Qiagen) and quantified using the NanoVue spectrophotomer (GE Healthcare). A total of 70 ug of DNA was sent to the High-Throughput Sequencing Facility at University of North Carolina. The library was prepared using the PacBioŐs 20Kb template prep protocol (PN_100-286-000-06) and the library was size-selected on using a range setting of 8000bp to 50,000bp.