Transcriptome sequencing of koala spleen
mRNA was extracted using a Qiagen RNeasy Mini Kit with Qiashredder columns and “on-column” RNase-free DNase1 digestion to eliminate genomic DNA. Sequencing samples were prepared with the Illumina TruSeq RNA Sample Prep Kit v2. Libraries were paired-end sequenced on an Illumina HiSeq 2000.