GSM1845291: ChIP-Seq oocyte K4Me3 zebrafish; Danio rerio; ChIP-Seq
In order to deyolk oocytes (where yolk mass were highly abundant), dechorionated oocytes were transferred into a 1.5ml tube and washed with PBS for several times, then disrupted with 200ul tips. The cells of oocytes were spun down at 5000rpm for 5 min at 4°C. The remaining cells were incubated in lysis buffer ChIP-Seq libraries were prepared using TELP developed by PengXu et al. with slightly modification