GSM4704471: Nasal associated lymphoid tissue, XCR1+ dendritic cells, replicate 3; Mus musculus; RNA-Seq
RNA from sorted NALT and cLN DC subsets was isolated using the microRNeasy Micro Kit (Qiagen) with on column DNase digestion according to manufacturer's instructions. First strand cDNA synthesis and cDNA amplification were performed using the SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (Clontech Laboratories) according to manufacturer's instructions. Complementary DNA libraries were prepared and indexed separately using the Nextera® XT DNA Library Preparation Kit (Illumina) following manufacturer's instructions.