GSM3581884: IL-4-positive Lung; Mus musculus; RNA-Seq
CD4+ T cells were sorted into single cell suspensions and sample RNA was isolated using the Quick-RNA Microprep kit (Zymo Research) according to the manufacturer's protocol. Single cell RNA sequencing was performed using the Chromium Single Cell 3' Library and Gel Bead Kit v3 (10x Genomics) according to the manufacturer's protocol using the Chromium Controller. Single cell libraries were sequenced on an Illumina Novaseq 6000.