GSM2627594: Idx19 Colon SW480; Homo sapiens; Bisulfite-Seq
Each genomic DNAs are obtained from other groups and extracted using Quiagen genomic DNA extraction Kit (69504) Entire amplicons obtained from eight rounds of multiplex PCR were pooled together in equal volumes. For sequencing library construction, we performed a series of enzymatic reactions such as 5’-end phosphorylation, adaptor ligation, and two additional cycles of PCR to attach barcode and other modules, as described elsewhere (Min et al., 2016). Every intermittent purification was conducted using ExpinTM PCR (GeneAll). The sequencing libraries were pooled with equal amount and applied to a multiple parallel sequencing in the same flow cell using Illumina Hi-seq 2500.