GSM4104495: small RNA-seq in the Ago2 deletion mESC, biological replicate 1; Mus musculus; ncRNA-Seq
Total RNA were isolated from mESCs via Trizol reagent RNA-seq library was constructed via the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (E7420S). The small-RNA-seq libraries were prepared using the NEBNext Multiplex Small RNA Library Prep Set for Illuminia (Set 1) (E7300S). The Trim71 CLIP-seq was performed using the previously established HITS-CLIP protocol (Moore et al., 2014). CLIP-seq, RNA-seq, small RNA-seq