NextSeq 500 paired end sequencing; ChIP-seq of Mediator subunits in Mediator mutants and wild type yeast
ChIP-seq of Mediator subunits in Mediator mutants and wild type yeast
Yeast were grown at 30˚C in YPD to A600 of 0.8; rapamycin added to 1 microgram/ml and incubated 1 hr additional ChIP performed by standard methods.  Antibody incubation was overnight at 4 C. The NEB Next Ultra II DNA library prep kit was used according to manufacturer's instructions.