GSM5076804: Day1_050_BLD_R1_Next; Homo sapiens; RNA-Seq
Hematoma effluent and blood samples were collected and scRNA-seq was performed using the Seq-Well protocol (Gierahn et al., 2017). Briefly, cells were loaded onto arrays with polyA capture beads (ChemGenes), sealed with a membrane, and subject to cell lysis. RNA was captured, beads were collected, and reverse transcription with Maxima H Minus (ThermoFisher) was performed. Whole transcriptome amplification product was purified and used as input for library construction. Bulk RNAseq for CD4 sorted cells was performed as described in GSE163256. Sequencing libraries were prepared using Nextera XT (Illumina).