GSM2197502: mir-60;spe-9, Day7, Seq-2; Caenorhabditis elegans; RNA-Seq
Animals collected were first washed with M9 buffer 3 times, then incubated in M9 with rotating for 30 minutes to allow them to digest bacteria within the intestine. Total RNAs were purified using mirVana miRNA Isolation Kit (Ambion/Life Technologies), according to the manufacturer's instruction. cDNA libraries for RNA-sequencing were established from 4 μg of total RNAs for each using TruSeq Stranded mRNA Sample Prep Kit (Illumina) with indexed adapters, according to the manufacturer's instruction.