GSM1843704: AR501_L3_RNAseq; Caenorhabditis elegans; RNA-Seq
Total RNA was prepared using TRIZOL and Qiagen RNeasy spin kit. RNA-seq was performed using a standard protocol (NEBNext Poly(A) mRNA Magnetic Isolation Module E7490, NEBNext Ultra RNA Library Prep Kit E7530 or E7420, and Agencourt Ampure XP beads). The resulting fragmented cDNA was ligated to Illumina multiplexed adapters and sequenced on Hi-Seq2500 Illumina instruments as 50 bp single-end reads with 8 bp for multiplex adapter sequencing.