GSM5076382: human melanoma cell line-Mel501R-Das treated; Homo sapiens; RNA-Seq
Total RNA was extracted from melanoma cell lines using Nucleospin RNA kit following the recommandations of Macherey Nagel. Libraries were generated from 500ng of total RNAs using Truseq RNA Library Prep Kit v2 (Illumina) following the recommandations. The concentration of library was first quantified by Qubit2.0, then diluted to 1 ng/ul,  and the insert size was checked by Agilent 2100 and was further quantified by qPCR (library concentration > 2 nM). If the library is qualified, it will be sequenced on an NovaSeq 6000 Illumina platform according to  effective concentration and data volume and using NovaSeq 6000 Reagent Kits.