GSM3895573: Heart, MI 256 (scRNA-seq); Mus musculus; RNA-Seq
Single cell suspensions were processed using the 10X Genomics ChromiumTM Single Cell 3' Assay, during which the cells were partitioned into Gel Bead-In-Emulsions (GEMs) and polyadenylated (polyA) transcripts were captured. Cells were incubated in GEMs with primers containing an Illumina R1 primer sequence, a 16 bp 10X barcode, a 10 bp Unique Molecular Identifier (UMI) and a poly-dT primer sequence and full-length cDNAs were generated from the captured polyA transcripts. cDNAs were then amplified by PCR followed by enzymatic fragmentation and size selection for optimised amplicons. P5, P7, i7 sample index and R2 primer sequence were added during library construction via end-repair, A-tailing, adaptor ligation and PCR.