The transcriptome landscape of Lactococcus lactis: regulatory RNAs uncovered
RNA concentration was measured using a Nanodrop  (Thermo Scientific, address), after which the integrity of the 16S/23S rRNA and DNA contamination were assessed using an Agilent 2100 Bioanalyser (Agilent Technologies address). The RNA sample for the TEX-treated library was   sent to Eurofins MWG GmbH (Ebersberg, Germany) for rRNA depletion with the Terminator System from Epicentre , preparation of a 5’-fragment cDNA library [as described in 44,93], PCR amplification and library purification. Both ?? libraries were sequenced on an Illumina HiSeq2000 v3 platform (Illumina voluit + address), with a paired-end protocol and read length of 101 nt, resulting in a total output of 10.5 M reads. The RNA sample for the non-TEX treated library was sequenced at Otogenetics Corporation (Norcross, United States) on an Illumina HiSeq2000 v3 platform , with a ScriptSeq Complete Kit (Bacteria, address) including Ribo-Zero™ rRNA  removal and ScriptSeq v2 library preparation  for directional RNA-Seq , resulting in a total of 15.7 M reads.