GSM1570700: ex72hr_1; Mus musculus; RNA-Seq
Trizol RNA was isolated using Trizol according to manufacturer’s instructions, including optional step in protocol. RNA quality was determined by Agilent Bioanalyzer. RNA-seq libraries were prepared as previously described (Wamstad et al., 2012) or using an Illumina TruSeq kit. A final round of size selection by Agencourt AMPure XP beads was performed to remove small fragments such as primers. Sequencing was run on either an Illumina GA-2 or Hi-Seq (barcoded).