GSM4155077: NF_MTB_rep2; Mycobacterium tuberculosis; RNA-Seq
Total RNA was isolated by phenol-chloroform extraction after cell disruption with Bead Beater (BioSpec Products). After isolation, RNA was treated with Turbo DNase (Life Technologies) to remove traces of genomic DNA. RNA samples were depleted of both prokaryotic and eukaryotic rRNA with Ribo-Zero Gold rRNA Removal Kit (Illumina) (Samples NF_MTB_rep 1-3) or RiboMinus Transcriptome Isolation Kit, Bacteria (ThermoFisher) (Samples MTB_control_rep 1-3). Sequencing libraries were generated using the resulting ribosomal transcript-depleted RNA and NEBNext Ultra II Directional RNA Library Prep Kit (NEB) according to the manufacturers' protocol.