GSM5159059: WT MNase Replicate 2; Saccharomyces cerevisiae; MNase-Seq
Cells were harvested spheroplasted with zymolyase, and chromatin was subjected to MNase digestion. The digested chromatin was treated with Proteinase K and the DNA was subsequently phenol chloroform extracted and ethanol precipitated. The samples were treated with RNaseA and hosphatase, then purified using a Qiagen PCR purification kit. Libraries were prepared using the manufacturer's protocol for the Nugen Ovation Ultralow System V2 kit