Roquin binds microRNA-146a and Argonaute2 and regulates microRNA homeostasis
C57BL/6(B6) and Roquinsan/san (sanroque) mice were housed in pathogen free conditions at the Australian Phenomics facility at Australian National University (ANU). Roquin/Roquin-2-/- mice were housed in a pathogen-free barrier facility at the Helmholtz Zentrum, München. For bone marrow chimera reconstitutions, 2x106 cells were injected i.v in to 10-12 weeks old sublethally irradiated (500 cGy, X-rad) C57BL/6 Ly5a recipients and analyzed 12 weeks after reconstitution. The ANU Animal Ethics and Experimentation Committee approved all animal procedures. T cells from spleen and lymph nodes were enriched with an EasySep™ Mouse T Cell Enrichment Kit (Stemcell, Cat# 19751) to >90% purity. RNA was prepared using TRIzol (Life Technologies) and the concentration and integrity of total RNA was verified with an Agilent 2100 Bioanalyser prior to small RNA library construction and deep sequencing by Illumina HiSeq Technology (Beijing Genomics Institute at Shenzhen).