GSM3356667: THP1_C; Homo sapiens; ChIP-Seq
The pellets were sonicated and the chromatin was treated with Rnase, proteinase K and heat (65oC), followed by phenol and chlorophorm extractions, and ethanol precipitation. Pellets were resuspended in 10mM Tris, 1mM EDTA, and quantified in Nanodrop. Libraries were prepared using Illumina Hi-Seq, and mapped to the genome using BWA algorithm using default settings.