GSM4766881: CNT711; Rattus norvegicus; RNA-Seq
RNA was isolated using RNeasy mini kit (Cat. # 74104) from Qiagen. RNA quantity was measured with Qubit™ RNA HS Assay Kit (Thermofisher); the RNA integrity and quality were assessed using an Agilent Bioanalyzer using the Agilent RNA 6000 Nano kit. All RNA used had an RNA Integrity Number between 9 and 10. Libraries were prepared starting from 100 ng of total RNA using the Illumina® TruSeq® Stranded mRNA Sample Preparation Kits following the standard protocol. Libraries were sequenced using an Illumina HiSeq1500 platform. Approximately 50 million paired-end reads (2x125bp) per sample were obtained.