GSM5221039: NTT_ATACSeq_Rep2; Mus musculus; ATAC-seq
ATAC-seq was performed according to an adapted version adapted version of the Omni‐ATACseq protocol. Briefly, nuclei were isolated with nuclei isolation buffer (0.32 M sucrose, 3 mM CaCl2, 2 mM Mg Acetate, 0.1 mM EDTA, 10 mM Tris.HCl pH 8.0, 0.6% NP-40, 1mM DTT). After isolation, approximately 50,000 nuclei were resuspended in TD buffer with the Illumina Tn5 transposase (#15027865). The transposition reaction was performed at 37°C for 30 min. DNA fragments were purified from the reaction with the MinElute PCR purification Kit, Qiagen (# 28006) and amplified using the NEB Q5 High Fidelity PCR Mix (#M0492). Library quality was assessed using a Fragment Analyzer system (Agilent). ATAC-seq libraries were quantified using a KAPA library quantification kit and sequenced on a NovaSeq instrument in 50 bp paired‐end mode.