GSM1843481: WT Y258, empty vector control, replicate 3; Saccharomyces cerevisiae; RNA-Seq
Total RNA was isolated from yeast cells following the procedure of the NucleoSpin RNA kit (Macherey&Nagel) for up to 3 x 108 yeast cells, which dictates incubation with 50-100U of zymolyase for 1 hour at 30°C. The quality of resulting total mRNA was tested on 1% agarose gels. According to EMBL GeneCore platform; standard Illumina protocol