NextSeq 500 paired end sequencing; All-trans Retinoic acid promotes viral mimicry and antigen presentation in immunologically silent breast-cancer cells
All-trans Retinoic acid promotes viral mimicry and antigen presentation in immunologically silent breast-cancer cells
24h after seeding, cells were treated with vehicle or ATRA for 24h. cells were grown in DMEMF12, 5% FBS.  24h after seeding, cells were  treated with vehicle or ATRA (1 μM) for 24 hours. RNA was extracted with the RNeasy Kit  (QUIAGEN), according to the manufacturer instruction. RNA libraries were prepared for sequencing using TruSeq RNA Library Prep Kit v2 (Stranded) from Illimuna®