GSM2533000: E13.5KL_1_10; Mus musculus; RNA-Seq
cDNA synthesis was performed following the Smart-seq2 method described by Picelli et al. 0.1 ul ERCC spike-in RNA (1:1,000,000 dilution, Life, 4456740) was added to the 4 ul lysis buffer in single cell RNA-seq. 2.5 ng of cDNA was used to conduct library using “TruePrep DNA Library Prep Kit” (Vazyme, TD502).