GSM2198528: ATAC 0hr B1; Mus musculus; ATAC-seq
Growth media was removed and MEFs were lysed in hypotonic lysis buffer to isolate nuclei. Approximately 40,000 nuclei were used as input to the Nextera DNA Sample Preparation Kit (FC-121-1030). ATAC libraries were prepared using the standard library preparation protocol outlined in the Nextera DNA Sample Preparation Kit (FC-121-1030).