GSM3664701: A77+Nucleotides_24hrs_r2; Homo sapiens; RNA-Seq
Treated A375  cells were washed and libearse was used.  Total RNA was extracted with TrizolPlus RNeasy mini kit from Qiagen. Total RNA was depleted of ribosomal RNA with the RiboZero gold kit (Epicentre). Enriched mRNA was then applied to library preparation according to manufacturer's protocol (NEBNext Ultra). After repeated quality control for average DNA input size of 300 bp, the samples were sequenced on a HiSeq Illumina sequencer with 2x100bp paired-end reads.