GSM1570041: MUT1_MeDIPseq; Mus musculus; MeDIP-Seq
DNA was purified from cells using standard methods (digestion with proteinase K ON at 55C, phenol-chlorophorm extraction and precipitation with ethanol), sonicated to fragments with average size ~250 bp and the methylated DNA was captured with anti-5mC antibody. Sequencing libraries were prepared with NEBnext DNA library Prep Mastermix Set for Illumina (E6040S, New England Biolabs) and NEBnext Singleplex Oligos for Illumina (E7350S, New England Biolabs) following manufacturer instructions.