GSM2971135: IL2 IL4 TCR CD8 rep3_run3; Mus musculus; RNA-Seq
Total RNA was extracted using the the NucleoSpin II RNA isolation kit (#740955, Macherey-Nagel, Dueren, Germany) following the manufacturer's protocol Libraries were made from 200 ng input RNA using the RNA Library Preparation Kit (Kapa Biosystems #KK8420) and modified primers. Libraries were analyzed for size and quality using the High Sensitivity Bioanalyzer Kit (Agilent #5067-4626) and sequenced with on Life Technologies Ion Proton at the NJH Genomics Facility.