GSM4391958: GEneuron8; Mus musculus; RNA-Seq
Four GI-ERαvlVMH neurons and four GE-ERαvlVMH neurons (from two different mice) were manually collected and suspended in PBS buffer. Cell lysis, first-strand cDNA synthesis and cDNA amplification were performed according to the manufacturer's instructions of SMART-Seq v4 Ultra Low Input RNA Kit (Clontech). Amplified cDNA was purified by Agencourt AMPure XP Kit (Beckman Coulter). The Nextera Library Prep Kit (Illumina) was used to prepare libraries for sequencing (100 bp paired-end, RNA-seq) on a HiSeq 2500 platform (Illumina).