GSM3289843: 6-8h_pros-neg_1; Drosophila melanogaster; ncRNA-Seq
Drosophila embryos were collected at time points 6-8h and 18-22h after egg laying.  Embryos were dissociated into single-cell suspensions, cells were fixed in 4% formaldehyde.  Fixed cell suspensions were immunostained under RNase-free conditions and FACS-purified using a FACSAria II cell sorter (BD Biosciences).  Marker-positive and marker-negative cell populations were collected in biological duplicates.  FACS-purified cells were subjected to cross-linking reversal and Proteinase K digestion prior to RNA isolation.  From both FACS fractions, RNA was extracted and prepared for strand-specific Illumina sequencing. NEBNext Small RNA (New England Biolabs) smallRNA-Seq, 1x51bp