GSM3790545: E15.5.cTEC; Mus musculus; ATAC-seq
DNA was extracted by incubating the cells in 50ul of cold lysis buffer (10 mM TRIS-HCl, pH 7.4, 10mM NaCl, 3 mM MgCl2, 0.1% Igepal CA-630) for 15 min while centrifuging (500 x g, 4°C) Pelleted nuclei were resuspended in transposition reaction mix with Tn5 Transposase (Illumina FC-121-1030) and incubated 45 min with gentle shaking at 37°C. Tagmented DNA was purified using SPRIselect beads (Beckman Coulter B23317) and amplified by PCR. Resulting libraries were size-selected and purified again by SPRIselect beads