GSM2970689: NKG2C+ VMAPQSLLL donor 81; Homo sapiens; RNA-Seq
Total RNA was purified from FACS-sorted NKG2C+ NK cells with RNeasy Plus Micro kit (Qiagen) according to the manufacturer's instructions. Illumina libraries were prepared using Smart-Seq v4 mRNA Ultra Low Input RNA Kit (Clontech) and Nextera® XT DNA Sample Preparation Kit (Illumina), with max 10ng of purified cDNA, according to the manufacturer's instructions and paired-end sequenced (2x75 bp) on an Illumina NextSeq500 device.