GSM3408831: drought_0h_poly(A)+ rep2; Arabidopsis thaliana; RNA-Seq
Total RNA was extracted with TRIzol (Invitrogen) and treated with DNase I (Ambion). Polyadenylated [poly(A)+] RNAs were isolated from total RNA through two rounds of purification with oligo(dT) beads (QIAGEN). Libraries for RNA-seq were generated using NEXTflex Rapid Directional qRNA-Seq Kit  (dUTP based) according to the manufacturer's instructions.