GSM3581781: PF_Z2; Fragaria x ananassa; RNA-Seq
RTotal RNA was extracted using a modified CTAB method, and treated with RNase-free DNase I (Takara, Dalian, China) to remove genomic DNA contamination. The total RNA quantity and purity were detected by Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0. The mRNA-seq sample preparation kit (Illumina, San Diego, USA) was used with 10 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols