Anaerobic fermentation_day1
Ion Torrent PGM Fragment libraries of 200 nt were generated according to the appropriate protocols (Ion Torrent PGM, Life Tech, USA). 1 g pooled total metagenomic DNA/RNA from each sample was used for library preparations. Ion Xpress Plus Fragment Library Kit and Ion Total RNA-Seq Kit were used. Adapter ligation and nick translation were performed by Ion Shear Plus Reagents Kit. Size selection was performed in 2% agarose gel, then library amplification was achieved by using Platinum® PCR SuperMix. ION Library TaqMan qPCR was used for quantitation, and Ion PGM 200 Xpress Template kit was used for the emulsion PCR.