GSM4102053: CT1.0-d4-Input; Homo sapiens; ChIP-Seq
Neural progenitor cell lysates were clarified from sonicated nuclei and SOX1 complexes were immunoprecipitated with SOX1 antibody (R&D AF3369, respectively. Protein A and G Magenetic Dynabeads were used to capture antibody bound protein-DNA complexes. The ChIP-enriched DNA samples were treated with end-repair of the DNA, adding “A” bases to the DNA, ligating sequencing adapters to DNA fragments, amplifying adapter-modified DNA by PCR and gel purification for ChIPseq using NEBNext Ultra DNA Library Prep Kit for Illumina. Purified libraries were sequenced on Illumina HiSeq X-ten platforms.