Oxford Nanopore MinION WGS of  Escherichia coli CFT073
ONT sequencing libraries were  prepared by multiplexing DNA extracts from four isolates per flowcell using  the SQK-LSK108 and EXP-NBD103 kits according to the manufacturer's protocol  with the following amendments: input DNA (1.5g) was not fragmented, 2ml  Eppendorf DNA LoBind tubes (Eppendorf, Hamburg, Germany) were used, all  reactions were purified using 0.4x Agencourt AMPure XP beads, incubation time  with Agencourt AMPure XP beads was doubled, elution volumes were reduced to  the minimum required for the subsequent step, and elution was heated to 37C.  Libraries were loaded onto flow cell versions FLO-MIN106 R9.4 SpotON and  sequenced for 48 hours.