Pisum sativum cv Cameor Apical node paired end library, stage B, Low-nitrate, Hydroponics
PolyA messenger RNA were isolated from total RNA by two successive purification steps on OligodT25 magnetic beads using the Dynabeads® mRNA Purification Kit (Invitrogen, Paisley, UK). Double strand cDNA synthesis was done using the Superscript Double-Strand cDNA synthesis kit (Invitrogen). Then, 500 ng to 1 mg cDNA per samples were used to prepare cDNA libraries. Fragmentation was done using Covaris (Duty Cycles 10 %, Intensity 5, 200 cycles per burst, 90 sec). Libraries were prepared using the kit NEBNext® DNA Library Prep Master Mix Set for Illumina®. Libraries were paired-end sequenced as recommended by Illumina at the rate of one lane per library.