GSM4704970: hnRNPC-IP in failing heart (patient1); Homo sapiens; RIP-Seq
For immunoprecipitation of hnRNPC RNA complexes, 100 mg tissue from the human heart apex was used for each IP and control sample.After thawing, heart samples were washed, dissected, cross-linked and mechanically homogenized in lysis buffer. After sonication the sample was centrifuged and the supernatant was incubated O.N with antibody-conjugated-magnetic beads. After 3 washes reverse cross-linking was performed and proteins were digested by Proteinase K. RNA was extracted following TRIzol extraction protocol. RNA libraries were prepared for sequencing using standard Illumina protocols