GSM3895163: SRDX_Dark-2_stranded_mRNAseq; Arabidopsis thaliana; ssRNA-seq
Whole seedling were collected (40 seedllings) and snap-frozen in liquid nitrogen. Dark samples were collected under safe green light RNA was extracted with RNeasy Micro Kit (Qiagen cat. 74004) Libraries were prepared according to Illumina's instructions accompanying TruSeq Stranded mRNA Library Prep Kit. Briefly, total RNA (0.5µg) from each sample was used to isolate poly(A) mRNA. Steps involved in library preparation were mRNA fragmentation, first strand cDNA synthesis followed by second strand cDNA synthesis and adaptor ligation, cDNA fragments isolation and amplification. 