Bulk RNASeq of the CHO K1 DP12 cell line: Replicate 1
Total RNA was isolated from cells using Trizol (Invitrogen, cat. no 15596026) following manufacturer instructions. For each sample, 2.5 g of total RNA were poly(A) enriched with the Seq-Star poly(A) mRNA Isolation Kit (cat. no AS-MB-006) and RNA libraries were prepared using the KAPA Stranded RNA-Seq library preparation kit (Kapa Biosystems, cat. no KK8401) according to manufacturers specifications.