GSM3723710: MV4-11_Par_RNA-seq_rep1; Homo sapiens; RNA-Seq
MOLM-13 cells were harvested at indicated time points and washed with 1x PBS. Total RNA was extracted using RNeasy Plus Mini Kit (Qiagen) and QIAshredder (Qiagen). We isolated poly(A) mRNA using magnetic isolation method (NEXTFLEX, Bioo Scientific) using ~1 ug of total mRNA. To generate RNA-Sequencing libraries, we used NEXTFLEX Rapid Directional RNA-Seq Library Prep Kit (Bioo Scientific) and NEXTFLEX RNA-Seq barcodes (Bioo Scientific), which were carried out according to manufacturer's protocol. RNA-Seq barcoded libraries were then sequenced using Hi-Seq 4000 (single end, 50 cycles).