GSM3409445: ATAC-Nf Rep1; Drosophila melanogaster; ATAC-seq
Cells were lysed and nuclei pelleted. Nuclei were subjected to tagmentation (Nextera kit, Illumina). PCR amplification of purified DNA was performed using forward and reverse indexing primers and HotStart KAPA ready mix (13 cycles). Fragments between 200-700bp of the amplified and purified libraries were size-selected using a Pippin Prep instrument.