RNA-Seq of human neurons cultures transduced with an adenoviral vector containing PAX5 cDNA
~1,500,000 human neurons were seeded and cultured 24h prior to adenoviral transduction using PAX5 cDNA and subsequent RNA extraction. Paired-end RNA-seq was performed using bulk RNA purified by polyA selection and paired-end reads were aligned using Galaxy's web-based HISAT2 alignment tool.