GSM2198230: Kit_6h_CaptureC_merge; Mus musculus; OTHER
After cell fixation with 1% formaldehyde for 10min and cell sorting as described above, Capture-C was performed with a double-capture procedure (Davies et al. 2016). Chromatin was digested using DpnII. We used biotin labelled DNA oligos to pull down the target restriction fragments. Capture-C libraries were sequenced on Nextseq500 with 2x75 bp paired end sequencing.