Illumina HiSeq 2500 sequencing; RNA-seq of human head and neck cancer cell line SQD9 knock-out for TMEM45A exposed to cisplatin
RNA-seq of human head and neck cancer cell line SQD9 knock-out for TMEM45A exposed to cisplatin
SQD9 cell line (RRID:CVCL_D774) Medium MEM supplemented with 10% FBS and glutamine 100µM of cisplatin in once during 24h in CO2 independent medium with shTMEM45A (gene expression KO) RNA extract using the RNeasy Mini Kit(74104-Qiagen) and the Qiacube autamation (Qiagen)  IlluminaTruSeq stranded total RNA kit