GSM4766817: D05_A3 scRNA-seq; Homo sapiens; RNA-Seq
Cells were dissociated with acutase and washed twice in wash buffer (0.04% Bovine Serum Albumin in Ca2+/Mg2+-free PBS), filtered using 40um strainer and counted on the Countess II automated cell counter (Thermo Fisher). For each cell population, 10,400 cells were loaded per lane on the 10x Genomics Chromium chip, with the goal of capturing 6,000 cells. Library construction by 3' prime V2 chemistry Chromium single cell gene expression kit.