GSM4704581: WT Zebrafish 2; Danio rerio; RNA-Seq
Zebrafish – zygote stage embryos were injected with sgRNA-Cas9 ribonucleoproteins and grown to adult stages. Germline transmission of targeted deletion was performed by PCR and 1 desired founder animal (F0) was identified. The F0 was outcrossed to Wt animals to generate F1 heterozygous animals. F1 animals were incrossed and the resulting offspring was grown 28 hours stage embryos. Heads were dissected for PCR-genotyping and the trunks of 2 WT and 2 HOM embryos were processed for RNA extraction. RNA-seq library prepared with TruSeq Stranded mRNA (Illumina)