Illumina HiSeq 4000 sequencing; IMPC (international mouse phenotyping consortium) high throughput RNA seq of 1143 samples from 106 IMPC knockout lines
IMPC (international mouse phenotyping consortium) high throughput RNA seq of 1143 samples from 106 IMPC knockout lines
Trizol extraction as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina-compatible (GAIIx and HiSeq2000 platforms) RNA-seq libraries as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426