GSM3665093: E07B-3077680-12C53-TimePoint04-BoneMarrow-20; Plasmodium knowlesi; Macaca fascicularis; RNA-Seq
Please note that the RNASeq data for this experiment was produced across three specimen types (venous whole blood (WB), bone marrow (BM), and spleen tissue (SP)) using RNAlater and PAXgene sample collection and storage technologies.  Please carefully review the two README files provided as supplementary files with this dataset for the details on the methods used for sequence generation and abundance measures.  README file names are as follows:  E07M99YSMfKnXXZZ_06062018-Readme_MULTIPL_GEO.txt  E07M99FGMfKnXXZZ_06292018-Readme_MULTIPL_GEO.txt Approximately 1 μg of total RNA per sample was converted to double-stranded cDNA using poly-A beads to enrich for mRNA, and Illumina TruSeq Stranded mRNA Sample Prep kits to generate strand-specific libraries.   As a quality control, 92 spike-in RNAs of known concentration and GC proportions (ERCC Spike-In Control, Life Technologies) were added to constitute approximately 1% of the total RNA for each library.  Adapters were ligated to facilitate multiplexed sequencing on an Illumina HiSeq3000 at the Yerkes Genomics Core, aiming for 50 million paired-end 100 base pair (bp) reads per library. RNA was extracted using Tempus-Spin RNA isolation kits. The quality of all RNA samples was confirmed using a Bioanalyzer, with an RNA Integrity Number (RIN) greater than 8 recorded for all samples."