Illumina HiSeq 2000 sequencing; RNA profilling of non-small cell lung cancer
RNA profilling of non-small cell lung cancer
Study participants and phenotypes Between March 2010 and August 2011, we prospectively recruited newly diagnosed and previously untreated lung cancer patients that were referred for thoracic surgery with curative intent. Subjects were only eligible for inclusion if surgical resection of the tumor occurred prior to any form of medical treatment. Tissue was collected immediately after lobectomy/pneumonectomy. Total RNA was extracted from six 30-μm sections of frozen whole tumor and adjacent lung tissue using the Qiagen-RNeasy Mini kit according to the supplier's instructions (Qiagen, Hilden, Germany). The quantity of DNA-free total RNA was measured using a Nanodrop-2000. Illumina sequencing libraries were prepared according to the TruSeq RNA Sample Preparation Guide following the manufacturer's instructions.