E. coli FusAP610T population replicate 1 grown in 15 µg/ml kanamycin for 5 passages (transfers) Read 1
Genomic DNA was extracted from both control and experimental populations using the GenElute Bacterial Genomic DNA Kit (NA2120, Sigma-Aldrich). Integrity of the extracted gDNA was checked on agarose gel, and quality and concentration were checked using NanoDrop UV-Vis Spectrophotometer (Thermo Scientific). Library preparation for deep sequencing was carried out using the Truseq Nano DNA Library Preparation Kit (FC-121-4001, Illumina).