GSM3272796: mRNA_PAPA_NDN_1; Homo sapiens; RNA-Seq
Normal density neutrophils were isolated by dextran sedimentation from control and PAPA patients. LDGs were isolated by negative selection from the PBMC fraction. Cells were resuspended in TriPure and RNA was isolated using the Zymo RNA Miniprep Kit. cDNA libraries were prepared using the TruSeq® Stranded mRNA NeoPrep™ Kit (Illumina) RNA seq data were generated on Illumina HiSeq 3000 instrument according to the manufacturer's instructions