Mcap sperm for genome sequencing
We collected a mixture of egg sperm bundles immediately upon their release and the sperm fraction was removed by pipetting to a new tube and was cleaned by a series of three rinse-and-spin steps, with samples rinsed with 0.2 micro m filtered seawater and centrifuged at 13,000 rpm for 3 min and snap frozen in liquid nitrogen. Sperm bundles were ground to a powder in liquid nitrogen and DNA was extracted with the Qiagen Genomic -tip 100/G kit according to the manufacturers instructions. DNA concentrations were measured on a Qubit instrument and the sample sent to the DNA Link Sequencing Lab 37 for sequencing on an Illumina HiSeq 2500 platform using a 100 x 100 paired-end cycle kit.