GSM2196467: Rat 04 ovary; Rattus norvegicus; RNA-Seq
Immediately following removal, ovary tissue was flash frozen on liquid nitrogen and stored at -80 oC.  To purify total RNA, tissue was thawed in TRIzol and homogenized using mechanical disruption.  RNA was then purified using a commercial spin column kit, according to manufacturer’s instructions (Direct-zol RNA MiniPrep, Zymo Research R2053), and flash frozen on liquid nitrogen Following poly(A) selection, cDNA libraries were prepared using the HiSeq SBS Kit (v. 4) with 4x-mutiplexing and the quality was verified by Bioanalyzer analysis. Sequencing was performed on the HiSeq 2500 platform, in paired-end 125 base-pair mode.  All four independent ovary samples were run in a single lane, which generated a total of 47,664 Mbp of sequence across 1.9 x 108 clusters with mean Q-scores all above 35.