GSM2800499: EF3; Entamoeba histolytica; RNA-Seq
Total RNAs were isolated by Trizol reagent (Sigma) according to the recommendations of the manufacturer. Libraries were constructed using Truseq mRNA-Seq Library Preparation Kit (Illumina) following manufacturer’s recommendations and were quality controlled using Agilent Bioanalyzer. Sequencing was performed on a HiSeq 2500 (Illumina) to obtain 65 base single-end reads.