NextSeq 500 paired end sequencing; Identifying crossover locations in Arabidopsis thaliana wild type and taf4b Col × Bur F2 populations using genotyping-by-sequencing
Identifying crossover locations in Arabidopsis thaliana wild type and taf4b Col × Bur F2 populations using genotyping-by-sequencing
Arabidopsis thaliana rosette leaves were collected from 5-week-old plants and frozen at -20°C. Arabidopsis thaliana plants were grown under long day 16/8 hour light/dark photoperiods at 20°C, 60% humidity and 150μmol light intensity. DNA was extracted from rosette leaves using CTAB. Sequencing libraries were constructed as previously described ( Ziolkowski et al, 2017 Genes & Dev; Rowan et al, 2015 G3 (Bethesda); Yelina et al, 2015 Genes & Dev).