RNA-seq of CD8 T cells from peripheral blood
RNA was isolated using the Qiagen RNeasy micro kit (#74034) according to the manufacturer's protocol,RNA-seq libraries were prepared using the SMARTer Stranded Total RNA-Seq Kit for Pico Input Mammalian from Clonetech according to the manufacturer's protocol (#635007),The libraries were sequenced on an Illumina NextSeq machine using a 300 cycle high output flow cell (#15057929)