GSM4766905: H3K36me3.Sen.Rep1.PD76; Homo sapiens; ChIP-Seq
ChIP was performed as described previously from ~12E6 cells (Shah, P.P. et al. Lamin B1 depletion in senescent cells triggers large-scale changes in gene expression and the chromatin landscape. Genes Dev 27, 1787-99 (2013).). Briefly, cross-linked cells were lysed, and the chromatin was sheared to an average size of <500 bp using a Covaris S220 Ultrasonicator. 2ug of sonicated chromatin was used per immunoprecipitation reaction (see Table S3 for a list of antibodies), and 10% of the amount was saved as input. Immunoprecipitation was performed using protein A Dynabeads (Life Technologies). For ChIP-seq, 1ng of DNA from immunoprecipitation and input was used to prepare libraries with the NEBNext Ultra II kit (New England Biolabs). The libraries were checked for quality and quantity by Bioanalyzer (Agilent) and qPCR (Kapa Biosystems) respectively. Multiplexed libraries were sequenced on a single lane of the NextSeq 500 platform (Illumina).