GSM1566843: Dam_Rep1_HEK293T; Homo sapiens; OTHER
DNA was isolated and prepared as described in Vogel et al. 2006 (Vogel, M.J., Peric-Hupkes, D., and van Steensel, B. (2007). Detection of in vivo protein-DNA interactions using DamID in mammalian cells. Nat. Protoc. 2, 1467–1478.) Sequencing libraries were prepared as described in Aprea et al. 2013 DamID-Seq - is an alternative to ChIP-Seq Sequencing for investigating DNA-Protein Interactions if no antibody is available.  DamID is based on the expression of a fusion protein consisting of a protein of interest and DNA adenine methyltransferase (Dam). This leads to methylation of adenines near sites where the protein of interest interacts with the DNA. These methylated sequences are subsequently amplified by a methylation-specific enrichment protocol and identified by NGS.  (Vogel, M.J., Peric-Hupkes, D., and van Steensel, B. (2007). Detection of in vivo protein-DNA interactions using DamID in mammalian cells. Nat. Protoc. 2, 1467–1478.)