GSM3408876: amiR-NAT2680 [ncRNA-seq]; Arabidopsis thaliana; ncRNA-Seq
Total RNA were extracted by Trizol reagent (Invitrogen) followed by PEG precipitation to enrich low molecular-weight sRNAs. The resulting sRNAs were resolved on a 15% denaturing PAGE gel, and visualized by SYBR-Gold (Invitrogen) staining. Gel slices within the range of 18-30nt were excised, and the RNAs were eluted and purified for small RNA library contruction. Libraries for sRNA-seq were generated using NEBNext Small RNA Library Prep Set for Illumina (NEB) according to the manufacturer's instructions.