IcRV-I.melas-I-75-2016
RNA extracted was quantified spectrophotometrically. RNA was subjected to retro-transcription with the SuperScript III Reverse Transcriptase (Invitrogen). Double stranded cDNA (ds cDNA) was then synthetized using the NEBNext® mRNA Second Strand Synthesis Module (Euroclone), purified with magnetic beads (Agencourt AMPure XP, Beckman Coulter) and finally quantified with the Qubit dsDNA HS assay kit (Thermofisher). Libraries were prepared using the Illumina Nextera XT DNA Sample Preparation kit (Illumina®), whereas fragment size selection was performed using the Agencourt AMPure XP (Beckman Coulter). The quality of the libraries and the fragment size were checked using Agilent 2100 Bioanalyzer (Agilent High Sensitivity DNA kit, Agilent Technologies).