GSM1203193: emf2b rep2; Oryza sativa; RNA-Seq
Inflorescence 7 stage, according to (Itoh et al., 2005) (0.3-1.5 cm) was dissected from wild type Dongjin and placed in liquid Nitrogen. Frozen tissue was homogenized using the Mini Beadbeater (BioSpec Products, Inc.). RNA was extracted with Trizol Reagent (Invitrogen) using the manufacturers recommended protocol. RNA was further purified with the QIAGEN RNAeasy Plant Mini kit (QIAGEN) following the manufacturers protocol for RNA cleanup including the optional DNase digestion. RNA was quantified on the ND 1000 Nanodrop Spectrophotometer (Thermo Fisher Scientific, Inc.). cDNA was synthesized using the Ovation RNA-seq System (NuGEN) with a starting input of 10 ng of RNA. RNAseq libraries were created with the Encore NGS Multi Library Prep I kit (NuGEN).  Forty cycles of single-read Illumina high throughput sequencing was performed on an Illumina Genome Analyzer IIx