GSM3580702: H12_neg_ATAC_R3; Gallus gallus; ATAC-seq
FAC-sorted cells were lysed (10mM Tris-HCl, pH7.4, 10mM NaCl, 3mM MgCl2, 0.1% Igepal) and tagmented using Illumina Nextera DNA kit (FC-121-1030) for 20mins at 37°C. Tagmented DNA was amplified using NEB Next High-Fidelity 2X PCR Master Mix for 11 cycles. Tagmentation efficiency was assessed using Agilent Tapestation. Three biological replicates were obtained for each stage. Libraries were prepared using Illumina Nextera DNA kit and sequenced on Illumina NextSeq500 using 40bp paired end reads