HOE-DYLAN 5 marine particle metagenome from brine solution
A HOE-DYLAN 5 sediment trap filled with brine solution and screened with 335 micron mesh was deployed at 500 meters at station ALOHA in the North Pacific Subtropical Gyre from July 14,2012-July 26,2012. It was filtered onto a 0.2 micron filter, preserved with RNAlater and frozen at -80C. Genomic DNA representing the 0.2-335 micron fraction was isolated from frozen filters using a modified MOBIO Powerwater kit protocol and prepared for sequencing using the Illumina Nextera XT DNA sample preparation protocol. The library was dual-indexed according to Illumina's low-plexity pooling guidelines. This library was pooled with 9 others on a 300 bp paired-end sequencing run on a MiSeq instrument using MiSeq reagent kit version 3.