GSM2970256: Screen3-4round GFP negative; Homo sapiens; OTHER
Cells were sorted via flow cytometry, genomic DNA was extracted, and PCR for the sgRNA sequences was performed. Illumina adaptors and barcodes were incorporated into the PCR primers.PCR amplified libraries were generated with independent barcodes to allow multiplexing. 50% PhiX DNA was included in the sequencing run, as low diversity libraries were expected. qiagen_PCR