GSM3665222: Glia 3; Drosophila melanogaster; RNA-Seq
Flies were snap frozen on dry ice followed by dissection of heads. Heads were homogenized in trizol and phase separated. The aqueous phase was then purified using the Qiagen Rneasy kit per the manufacturer's protocol. Stranded libraries for next-generation sequencing were prepared in the Harvard Biopolymers core facility by depleting total RNA of ribosomal RNA using the Directional RNA-Seq Wafergen system (Wafergen). All RNA samples were run on Agilent 2100 TapeStation D1000 High Sensitivity ScreenTape to assess concentration and size distribution prior to library creation and again after library creation. Libraries were also subjected to qPCR analysis for quality control. Libraries were then paired-end sequenced on an Illumina NextSeq 500 instrument.