RNA-Seq of Ciona robusta cell precursors
A paired-end sequencing was done, in which short reads are obtained from ends of DNA fragments for ultra high-throughput sequencing. Prior to further analysis,  was performed on the sequencing data: Check of quality reads, Reads mapping, Transcriptome expression quantification and differential expression analysis. The mapping of paired-end reads was performed using the bioinformatics tool STAR (version 2.5.0a) (Dobin et al., 2013) with the standard parameters. The reference track was the assembly Ciona_intestinalis.KH.78 obtained from ENSEMBL.