GSM4767413: Hepatoblast - Day 8_TUN_100_TP-8_REP-B [Bas232]; Homo sapiens; OTHER
Cells were washed with PBS and lysed with 1x BNN lysis buffer (provided by BioSpyder) followed by incubation of 15 min at RT. Thereafter plate with cell lysates was sealed and stored at -80 degrees until shipment. Cell lysates were send to BioSpyder for TempO-seq targeted transcriptome analysis. mRNA in the lysates was hybridized with detector oligo mix, and amplified using barcoded primer pairs to add a unique tag for each of the samples. The detector oligo mix targets a set of genes which were selected based on the S1500+ gene list of NIEHS (Mav et al. 2018). This list contains 2736 genes that provide maximal toxicogenomic information on chemical perturbations that reflect general cellular responses. We supplemented this list with another 245 genes that are involved in hiPSC to hepatocyte differentiation, cellular stress pathways activated in PHH and were missing in the S1500 list. Sample amplicons were pooled and purified. The libraries were sequenced using a HiSeq 2500 Ultra-High-Throughput Sequencing System (Illumina, San Diego, CA).