Metagenome of the 0.22micron-2014-fraction-fosmid-library from Freshwater Reservoir Amadorio
The study aims to describe the microbiota of a freshwater reservoir using high throughput sequencing. The reservoir is called Amadorio and is located in the region of Alicante, Spain. 50L of sample was passed through a series of 20,5 and 0.22 micron filters. DNA was extracted from the 0.22 micron filter. A fosmid library was constructed with this DNA. Fosmids were pooled in batches of four 96-well plates (384 fosmids each) and 14 batches were sequenced by Illumina HiSeq 2000 (ca. 5500 fosmids in total).