GSM1844624: 7h-B1; Klebsiella pneumoniae; RNA-Seq
Total RNA was harvested using Trizol reagent following the protocol described by Toledo-Arana et al., 2009 (Toledo-Arana et al., 2009 - Nature 459: 950-956). Ribosomal RNAs were removed using the Bacteria Ribo-Zero Magnetic kit (Epicentre Biotechnologies) according to manufacturer's instructions. Illumina TruSeq Stranded mRNA Sample Preparation kit (Illumina) was used with rRNA depleted RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using the TruSeq Stranded mRNA Sample Preparation kit from Illumina according to the manufacturer's instructions.