sequence of Hardyhisp2
A stock of Hardyhisp2 (originally labeled HH2) virus that had been stored at -80C since 1999 was thawed and DNA extracted using the CTAB/phenol-chloroform method. After checking quality and quantity by agarose gel electrophoresis, total DNA was amplified using the Qiagen REPLI-g Mini Kit and the products checked by agarose gel electrophoresis and then purified using QIAamp DNA Mini Kits (QIAGEN Inc., Valencia, CA, USA). One microgram of DNA was sent to the Yourgene Health Co. (Taipei, Taiwan) for sequencing using the Illumina platform (HiSeq 2500 sequencer; 2250 bp paired-end reads).