Penicillium digitatum strain pdc102 dna sequencing
Fungal strain was grown in liquid media in PDB (Potato Dextrose Broth)  and chloramphenicol (250 mg L-1) in 250 mL Ehrlenmeyer flasks on an orbital shaker at 160 rpm for 48 h at 27 °C. Fungal liquid culture was vacuum filtered through wathman filter nu 10 using  Bichner vacuum, washed twice with sterile distilled water, filtered again, frozen and stored at -80 oC for subsequent DNA extraction.