GSM2971137: BD31; Homo sapiens; RNA-Seq
Lyphocyte suspensions were enriched for T cells using the Mojosort Human CD3 T cell isolation kit (biolegend), followed by which fluorescence activated cell sorting was performed using a BD Influx high-speed sorter or a BD Aria IIu sorter to isolate populations of interest. RNA was isolated from cell pellets using the RNeasy Mini Kit (Qiagen), and RNA concentration and quality was assessed using an Agilent 2100 Bioanalyzer instrument (Agilent Technologies). standard Truseq with poly-A pulldown