16S rRNA amplicon sequencing of Wara
The V4 hypervariable region of the 16S rRNA gene was amplified using primers 515F and 806R. PCR reactions were carried out with Phusion High-Fidelity PCR Master Mix (New England Biolabs, USA). The libraries generated with NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, England) were sequenced using paired-end Illumina sequencing (2250bp) on the HiSeq2500 platform (Illumina, USA).