GSM3896390: BHHVK2BBXX-2; Homo sapiens; RNA-Seq
Smart-seq2 extraction protocol from Picelli et al, Nature Protocols 2014 (doi:10.1038/nprot.2014.006) RNA was bead-purified from individual cells followed by cDNA synthesis by reverse transcription, template switching and preamplification using KAPA HiFi HotStart ReadyMix.  Indexed libraries were then made by tagmentation and pooled.  Typically, libraries from 75-80 cells were pooled per lane and sequenced with an Illumina HiSeq 3000/4000 paired-end cluster kit on a HiSeq 4000.