Illumina HiSeq 2000 paired end sequencing; RNA sequencing of Oryza sativa ssp. indica of inbred and derived epilines
RNA sequencing of Oryza sativa ssp. indica of inbred and derived epilines
The sample material was 12 pooled fifth leaves of individual plants with three biological replicates for the control line and the epilines LR1, LR2, and LR3, which belongs to the 4th selfing of the EUE selection pedigree.   Epilines LR1,LR2, and LR3, and the control line were grown in soil in a growth chamber at 26 C / 21 C (day / night) for 24 days with a 16 h light/8 h dark regime (light intensity was 300 mol m-2 sec-1, the relative humidity was kept at 71%). RNA isolation was performed using the Spectrum™ Plant Total RNA kit (Sigma-Aldrich) according to manufacturer's instructions.    The RNA-seq was done by ServiceXS B.V. (Leiden, The Netherlands). Complementary DNA sequencing libraries were prepared with the Illumina mRNA-seq Sample prep Kit according the Illumina protocol “Preparing Samples for Sequencing of mRNA” (1004898 Rev.D). DNA sequencing using the Illumina HiSeq 2000 (Solexa) was performed according manufacturer's protocols.