GSM2195933: Proximal S1 intestine in zebrafish 2 weeks after SBS surgery #1; Danio rerio; RNA-Seq
Full thickness section of intestine was removed, and RNA was harvested using Trizol reagent. RNA integrity was checked with the PIco chip in the BioAnalyzer. Kapa stranded RNA kit was used with 1 ug of total RNA for the construction of sequencing libraries. ERCC spikes ins were added based on manufacturer recommendations