GSM2198184: V446_sRNAs; Botrytis paeoniae; OTHER
Fresh mycelia were dried by pressing with sterile filter paper and total RNA was purified from 1 g of dry mycelia using TRIZOL reagent (Invitrogen) as described (Rodríguez-García et al. 2014) 5-10 ug of total RNA was used for library preparation as described (Al Rwahnih et al. 2011) and subjected to deep-sequencing using the Illumina platform (HiSeq2000, 2 x 100 bp length, IGA Technology Services, Udine, Italy, http://www.igatechnology.com) (Donaire et al. 2016)