Leaf transcriptome of Camellia oleifera JG04
Leaf samples were collected from flowering wild oil-tea camellia at different elevations within 3 hours in the afternoon. Three to five fresh leaves without obvious damage were randomly picked from each plant, covered by aluminum foil and immediately placed in a vacuum bottle with liquid nitrogen. Latitude, longitude and elevation of each sampling plant were recorded. At the same time of sampling, air temperature next to each sampling plant was measured. Each leaf was mixed with liquid nitrogen and grinded into fine powder. About 100 mg tissue powder of each leaf was used for RNA extraction. Total RNAs were extracted using the EASYspin Plus Plant RNA Kit (Aidlab, Beijing, China). To account for the gene expression variations among leaves of the same plant, the RNAs from two leaves of the same plant were equimolarly pooled and used as a single sample for the transcriptome sequencing. cDNA libraries were constructed from RNA samples for Illumina paired-end (PE) sequencing following the Illumina protocol.