The dynamic DNA methylation cycle from egg to sperm in the honey bee Apis mellifera
Fragments were end repaired (Illumina) as recommended by the manufacturer. Repaired fragments were ligated with methylated sequencing adaptors using a paired end adaptor oligo kit and oligo mix (Illumina). Ligated fragments were selected by gel electrophoresis and fragments of 360 bp size extracted using a QIAquick gel extraction kit (Qiagen); size-selected fragments were bisulfite treated using an EZ-DNA methylation kit (Zymo Research) and enriched using a MethylMiner methylated DNA enrichment kit (Invitrogen); Libraries were amplified using T4 polymerase (Enzymatics) and sequenced on an Illumina HiSeq platform.