GSM3207869: Scavina 6 hai Control 3; Theobroma cacao; RNA-Seq
Two leaves from different plants were pooled for each biological replicate. ~9cm^2 pieces of tissue were cut from each of the two leaves and grinded in a mortar and pestle using liquid nitrogen. RNA was extracted using Invitrogen plant RNA reagent according to instructions. Libraries were barcoded and prepared using a Truseq Stranded mRNA Library prep kit according to manufacturer's instructions. Equimolar pools of libraries were sequenced on a HiSeq 2500 in Rapid Mode with 100nt single reads. A total of five rapid runs were used.