GSM3408314: WT2 sRNA <100: Infected human red blood cells with Plasmodium falciparum; Plasmodium falciparum; RNA-Seq
Parasites were extracted from infected red blood cells using saponin lysis. After centrifugation and washing Parasites were disrupted using a cell disruption bomb (25 minutes at 1750 psi) in order to release intact organelles. After removal of debris through centrifugation the organelles were pelleted via centrifugation (20 min at 23.000 x g) and resuspended in TriZol.  RNA was extracted with the standard TriZol method. Library was constructed as described in NEBNext Multiplex Small RNA Library Prep