S. cerevisiae: time course galactose
We performed an RNA-seq experiment for the diploid hybrid strain (CBS2888xYJM981) over a time course growth experiment where the strain was transferred from YP +2% glucose media to YP+ 2% galactose media. In more detail, we collected yeast from mid-log (OD ~ 0.5) in glucose media. We then spun down the culture and resuspended it in galactose media (OD ~ 0.1), we then collected yeast at 30 minutes, 1 hour, 2 hours, 4 hours, and 5.5 hours. These samples were placed in the -80 freezer for further processing. We extracted RNA from each time point using the Quick-RNA Fungal/Bacterial Kit from Zymo research. We constructed RNA-sequencing libraries using the KAPA mRNA hyperprep kits. These libraries were then sequenced on an Illumina MiSeq.