GSM2875694: AbaSeq_rep5; Homo sapiens; OTHER
Genomic DNA was extracted from the prefrontal cortex using QIAGEN's QIAamp DNA Mini Kit (QIAGEN, cat. # 51304). DNA was glucosylated by using UDP-glucose and T4-β-glucosyltransferase (New England Biolabs, cat. #M0357L). The DNA was then digested using AbaSI (New England Biolabs, cat. #R0665S) and custom biotinylated adaptors were ligated to DNA ends. The DNA was then sheared using the Covaris S220 Focused-Ultrasonicator (peak incident power = 140; duty % = 10; cycles per burst = 200; time = 120 s). Sheared DNA was captured using Dynabeads® MyOne™ Streptavidin C1 beads (Life Technologies, cat. # 65001). NEBNext™ End Repair Module (New England Biolabs, cat. #E6050L) and NEBNext™ dA-Tailing Module (New England Biolabs, cat. #E6053L) were used to generate blunt ended fragments and create an adenosine tail, respectively. A second set of custom adaptors were ligated to the DNA, followed by PCR amplification using NEBNext™ High-Fidelity 2x PCR Master Mix (New England Biolabs, cat. #E6013AA). Illumina TruSeq indices 1 through 12 were used to barcode samples. 50 cycle, single-read sequencing was performed on Illumina's HiSeq 2000. A minimum of 60,000,000 sequencing reads was obtained per subject.