GSM2535384: barc RNAi larval type I neuroblasts - replicate 1; Drosophila melanogaster; RNA-Seq
Type I neuroblasts were FACS sorted from wild type (TID:60100) or barc RNAi, and RNA was isolated using Trizol LS reagent. S2 cells treated with dsRNA against mCherry or barc were washed and RNA was isolated using Trizol. For both experiments: Total RNA was enriched for polyA mRNA, fragmented and first strand and second strand (dUTP, instead dTTP was used) was generated. For the neuroblast sample, library preparation was done manually using the Illumina Kapa library prep kit, and for the S2 cell sample, library preparation was done with a robot using the Illumina NEB ultra Kit. For both samples, UGDase treatment was done to confer strand specificity.