Metagenomes of near-shore sandy sediments incubated with weathered crude oil over 3.5 months
DNA sequencing libraries were prepared using the Illumina Nextera XT DNA library prep kit according to manufacturers instruction for dual-indexed libraries except the protocol was terminated after isolation of cleaned double stranded libraries.  Library concentrations were determined by fluorescent quantification using a Qubit HS DNA kit and Qubit 2.0 fluorometer (ThermoFisher Scientific) according to manufacturers instructions and samples run on a High Sensitivity DNA chip using the Bioanalyzer 2100 instrument (Agilent) to determine library insert sizes.  An equimolar mixture of each library was sequenced on an Illumina HiSEQ 2500 instrument  located in the School of Biology, Georgia Institute of Technology for 300 cycles (2 x 150 bp paired end rapid run).