GSM3722748: IgG treated_2; Mus musculus; RNA-Seq
Spleen cells were MACS sorted to isolate CD8 T cells by negative selection (STEMCELL kit). Then, DbGP33+ cells were FACS-sorted and resuspended in Trizol. RNA extraction was performed using the Agecourt RNA extraction kit as per the manufacter instructions, and extracted RNA was delivered to the NUSeq Core at Northwestern University in downtown Chicago. RNA libraries were prepared for sequencing using standard Illumina protocols