GSM4103461: IM-SR-C5_S5: Control biological replicate 5; Mus musculus; RNA-Seq
Murine right and left atrial tisse was flash frozen separately using liquid nitrogen. Bulk RNA was extracted using Trizol and submitted to either the Genome Technology Access Center (GTAC) at Washington University School of Medicine (St. Louis, MO; for RA) or the Moskowitz Laboratory at the University of Chicago (Chicago, IL; LA) for RNA sequencing. For the RA, after ribosomal RNA depletion, reverse-transcription with random-priming, library construction and multiplexing, samples were sequenced on an Illumina HiSeq 3000 using indexed libraries multiplexed at 6 samples per lane. For the LA, libraries were prepared from LA RNA starting with 1 μg per sample and using the mRNA‐seq Sample Prep Kit (Illumina) as per recommended instructions. After Ribozero purification and removing only ribosomal RNA, barcoded libraries were prepared according to Illumina's instructions (2013) accompanying the TruSeq RNA Sample prep kit v2 (Part# RS‐122‐2001). Libraries were quantitated using the Agilent Bio‐analyzer (model 2100) and pooled in equimolar amounts.