GSM3896453: SprV Mutant RNA Seq; Streptococcus mutans UA159; RNA-Seq
Total RNAs from exponential-growth-phase cultures of S. mutans UA159 and its isogenic sprV deletion mutant were prepared using the RNeasy kit (Qiagen) following the manufacturer's recommendations. Cell pellets were pretreated with the RNAprotect bacterial reagent to stabilize RNA. Prepared RNA was quantified spectrophotometrically and subjected to RNase-free DNase (Qiagen) treatment for 45 min. After removal of DNA, mRNA was enriched from the total RNA using the Ribo-Zero rRNA removal kit (llumina) according to the manufacturer's recommendations. Resulting mRNA was quantified fluorometrically and subjected to the Illumina paired-end 100-bp library preparation protocols as per the manufacturer's recommendations.