GSM2973234: X1488.T; Mus musculus; RNA-Seq
Tumors from tamoxifen-treated p53neo/R172H;R26CreER/GNZ mice were harvested right after the final MRI in which a response (growth vs shrinkage) was observed and flash frozen on dry ice. Total RNA was isolated using using TRIzol reagent (Life Technologies) following standard manufacturers protocol Total RNA was submitted to the M.D. Anderson sequencing core facility and barcoded illumina compatible libaries wre prepared using the Illumina TruSeq mRNA seq Kit, per the manufacturer's protocol. The libraries were multiplexed 4 per lane and sequenced on the HiSeq3000 sequencer using the 75 bp paired end format. the raw RNA seq readouts were mapped to the mouse mm10 assembly reference