RNA-Seq of Metisa plana: Egg
Egg samples was collected from an oil palm plantation in Jengka, Pahang, Malaysia and immediately flash-frozen. Total RNA was extracted using RNeasy mini kit (Qiagen) and genomic contamination was removed using DNA-free DNA Removal Kit (Invitrogen). cDNA was then constructed using NEBNext UltraII Directional RNA Library Prep Kit for Illumina (BioLabs) and sequenced using Illumina HiSeq 4000 at 150bp paired-end(Novogene)