RNA-Seq of Homo sapiens: saliva from Healthy Control (total cell-free biofluid RNA): Sample GCB13S5
"Library construction kit was NEBNext small RNA library prep (NEB). Type of starting material was total RNA. RNA isolation kit was miRNeasy (Qiagen). Qiazol:saliva ratio was 3:1, with 20uL chloroform for every 100uL Qiazol used. Cell removal method was low speed centrifugation (2600g x 15 minutes). Spike-in sequences were used:  >1|5012815 TGACATGGGACTGCCTAAGCTA >2|4430506 GATGGATATGTCTTCAAGGAC >3|3920531 TCATGGTCAGATCCGTCATCC >4|3474759 TACCAACCTTTCATCGTTCCC >5|2772989 TAAGATCCGGACTACAACAAAG >6|2099198 TGATTGGAAATTTCGTTGACT >7|1932106 TCCCAAATGTAGACAAAGCA >8|844587 TTGAATTGAAGTGCTTGAATT >9|722119 TTCTTCGTGAATATCTGGCAT >10|655988 TTAGATGACCATCAACAAACT >11|363690 TAATTTGGTGTTTCTTCGATC >12|331387 TGTTTGTTGTACTCGGTCTAGT >13|321657 AGCTCTGATACCAAATGATGGAAT >14|311970 TCGCTCTGATACCAAATTGATG >15|200063 CGAAACTGGTGTCGACCGACA >16|131490 TTCGATGTCTAGCAGTGCCA >17|113465 TCTAGCAGCTGTTGAGCAGGT >19|106634 GGTTCGTACGTACACTGTTCA >20|99631 TCCGGCAAGTTGACCTTGGCT >21|84955 TTATGTCTTGTTGATCTCAAT >22|70769 TGAGCCTCTGTGGTAGCCCTCA >23|65795 TCCTGTGTTTCCTTTGATGCGTGG >25|56250 TTGGCATTCTGTCCACCTCC >28|36527 ATGAATTTGGATCTAATTGAG >30|33572 ATCAGTTTCTTGTTCGTTTCA >35|29397 TTGATTCCCAATCCAAGCAAG >36|26252 TACGCATTGAGTTTCGTTGCTT >37|24916 TAAACTAATCACGGAAATGCA >38|22984 CCTTGGAGAAATATGCGTCAA >39|22435 TTTGCTTCCAGCTTTTGTCTC >54|13534 TCTAAGTCTTCTATTGATGTT >57|12681 TGATCTCTTCGTACTCTTCTTG >61|12170 TTCTTGCATATGTTCTTTATC >67|11555 TGTGTTCTCAGGTCACCCCTT >75|10105 TGGCTTGGTTTATGTACACCG >90|7532 TAATCCTACCAATAACTTCAGC >93|7348 TAAAGTCAATAATACCTTGAAG >96|7247 TGAAGCTGCCAGCATGATCTA >105|6953 TAGAATGCTATTGTAATCCAG >110|6652 TTGGTTACCCATATGGCCATC >132|4738 CAGCCAAGGATGACTTGCCGG >176|3027 TAACTAAACATTGGTGTAGTA >194|2697 TTCGAGGCCTATTAAACCTCTG >237|2044 TAGTCCGGTTTTGGATACGTG >247|1894 ACAAACACCTTGGATGTTCTT >254|1851 TTCTGCTATGTTGCTGCTCAT >312|1402 ATTGGTTCAATTCTGGTGTTG >368|1146 AGAATCTTGATGATGCTGCAT >511|775 TTTTGGAAATTTGTCCTTACG >515|765 CCCCAAATGTAGACAAAGCA >1406|143 TTTTTCCTCAAATTTATCCAA >1596|112 TCTTGCTTAAATGAGTATTCCA >3275|34 CAGCCAAGGATGACTTGCCGA >6870|13 TGTGTTCTCAGGTCACCCCTG >13326|8 TTTGATTCCAGCTTTTGTCTC"