GSM2970259: HCC1937_vector_OTX-015; Homo sapiens; RNA-Seq
Cell culture medium was removed. Total RNA was extracted by Rneasy Plus Mini Kit and checked with Agilent bioanalyzer 2100 for RIN values. Then mRNA was isolated with NEBNext Poly(A) mRNA magnetic isolation module. RNA library was prepared for sequencing using NEBNext Ultra Directional RNA Library Prep Kit for Illunima