GSM1568621: GATA1s ChIP-Seq; Mus musculus; ChIP-Seq
Isolated chromatin was sonicated and pre-cleared with isotype-matched mouse non-specific IgG.  Chromatin immunprecipitation was carried out with a HA-tag antibody (Santa Crruz Biotechnology, sc-7392).  The formaldehyde crosslinks were reversed by heating to 65-degrees overnight.  RNA and protein were removed by treatment with RNAse A and Proteinase K, respectively, and the DNA was isolated using the Qiagen PCR Purification Kit. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part#  IP-102-1001).