Title: Mechanisms of Th17 and Treg cell dysregulation in oral mucosal inflammation during HIV disease. Description:  Transcriptome data from gingival mucosa  showing underlying changes in the oral mucosal immune system of HIV+ patients on therapy.: Sample Sample#4
RNA was isolated  Illumina TruSeq RNA Sample Prep Kit and total RNA was used for the construction of sequencing libraries.RNA libraries were prepared for sequencing using standard Illumina protocols-mRNA was purified from total RNA using poly-T oligo-attached magnetic beads.NEB library: Then first strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase (RNase H-).