GSM3664264: Br7_3; Homo sapiens; RNA-Seq
Individual cells from CTC-WBC-clusters were mechanically separated with gentle micromanipulation (CellCelector, ALS). AF488/FITC-positive (or GFP-positive) and BV605-negative CTCs or AF488/FITC-negative and BV605-positive WBCs were immediately transferred into individual tubes (#321-032-501, Axygen) containing 2.5 μl RLT Plus lysis buffer (#1053393, Qiagen) and 1U/μl SUPERase In RNase Inhibitor (#AM2694, Invitrogen). Samples were immediately frozen on dry ice and kept at -80°C until further processing Libraries were prepared with Smart -Seq2 protocol