GSM3581473: Fibach_NFE2_rep1; Homo sapiens; ChIP-Seq
ChIP-seq was carried out using ChIP Kit (17-295, Upstate Cell Signalling, now Millipore) following the manufacturers instructions. Briefly 10^7 cells and nuclei were lysed for 10 minutes before chromatin was sheared by sanitation in a Bioruptor (High: 10 min, 30sec on, 30 sec off). Insoluable material was pelleted and supernatant was incubated overnight with the relevant antibody. Antibodies were then conjugated to protein A-agarose and washed. DNA was extracted using phenol-chloroform followed by ethanol precipitation with yeast tRNA carrier. Samples were submitted to Oxford Genomics Center for indexing and sequencing (50 bp Illumina Genome Analyzer II).