RNA-SEQ O. glumapeatula
Twenty-five micrograms of high-quality total RNA per sample were sent to the Yale Center for Genome Analysis (New Haven, CT, USA) for library construction, sequencing and data generation. Sequencing of the double-stranded cDNAs was carried out on a HiSeqTM 2500 device (Illumina Inc., San Diego, CA, USA) using the paired-end technology in a single run .