GSM3895275: KD_Rep2; Homo sapiens; RNA-Seq
1 μg of total RNA was subjected to library synthesis using the KAPA RNA-Seq library kit. 1 μg of total RNA was enriched for the Poly-A mRNA and reverse transcribed to double-stranded cDNA. The cDNA was end repaired, adenylated, and ligated with Illumina adaptors prior to 12 cycles of PCR. Sequencing was performed using 12pM hybridization to a 2x75 paired end flow cell.