GSM4391903: ATAC-Seq RTE_SETD2_wt_rep2; Mus musculus; ATAC-seq
50,000 murine renal tubular epithelial cells were used for the transposition reaction at 37 °C for 30 min. After purification of the DNA with the MinElute PCR purification kit (Qiagen), material was amplified for 5 cycles as described previously (Buenrostro et al., 2013). Additional PCR cycles were evaluated by real time PCR. Final product was cleaned by AMPure Beads at a 1.5x ratio.