GSM2872749: 33LI - treatment 3 - cymopol low dose; Mus musculus; RNA-Seq
12 h following the final treatment, the mice were euthanized in 100% CO2.  The tissues were harvested immediately, frozen on dry ice, and kept at -80 ºC until analyzed. RNA libraries were prepared for sequencing using standard Illumina protocols.    Illumina RNA library construction was performed at the Interdisciplinary Center for Biotechnology Research (ICBR) Gene Expression Core, University of Florida (UF). Quantitation was done on a NanoDrop Spectrophotometer (NanoDrop Technologies, Inc.), and sample quality was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Inc).  rRNAs first were removed started with 90ng of total RNA by ClonTech RiboGone –Mammalian-Low input ribosomal RNA removal kit for Human, Mouse and Rat Samples (cat#: 634848) following the manufacturer's protocol, then the depleted RNA were used for library construction with SMARTer Universal Low input RNA kit for sequencing (cat#: 634940) combined with Illumina Nextera DNA Library Preparation Kit (cat#: FC-121-1030) according to the user guide.