Illumina HiSeq 4000 paired end sequencing; RNA-seq analysis of Col-0 and nrf1-2 mutant under Fe sufficient and Fe deficiency condition
RNA-seq analysis of Col-0 and nrf1-2 mutant under Fe sufficient and Fe deficiency condition
10d 1/2 MS grown plants were treated on +Fe or -Fe for 2d and then whole roots were harvested for RNA isolation 10+2d vertical grown plants on 1/2MS For +Fe 50uM Fe was used and for -Fe 100uM Ferrozine was used Total RNA was isolated by sing the protocol provided by Ambion nirvana RNA isolation kit cDNA library were prepared by using random digestion