GSM4153469: Activated T cells T1D; Homo sapiens; miRNA-Seq
CD4+ T cells were extracted from peripheral blood using density gradient centrifugation and CD4+ MACS enrichment. CD4+ T cell subpopulations were FACS sorted. RNA was extracted using the miRNeasy Micro Kit (Qiagen). miRNA libraries were prepared for sequencing using the NEBnext Multiplex Small RNA Library Prep Set (New England Biolabs) according to the manufacturer's protocol.