GSM4704557: mouse_ssh_model_3; Mus musculus; RNA-Seq
Tumor samples were dissociated using the Papain Dissociation System (Worthington Biochemical) as previous described (see reference). Briefly, tumor samples were incubated in papain at 37 °C for 15 min, then triturated and the suspended cells were spun through a density gradient of ovomucoid inhibitor. Pelleted cells were then resuspended in 2 ml of Bambanker media (fisher Scientific # NC9582225) and frozen at -80 degrees for storage prior to scRNA-seq analysis, (Ocasio J, Babcock B, Malawsky D, Weir SJ, Loo L, Simon JM, et al. scRNA-seq in medulloblastoma shows cellular heterogeneity and lineage expansion support resistance to SHH inhibitor therapy. Nat Commun. 2019;10(1):5829.) 3' end 10x Genomics library prep (v2 and v3)