Illumina HiSeq 2500 paired end sequencing; RNA-seq of the nematode Ascaridia galli exposed to flubendazole against untreated control
RNA-seq of the nematode Ascaridia galli exposed to flubendazole against untreated control
Adult females of A. galli were collected from the small intestine of naturally infected laying hens before and on day three during a 7-day treatment period with FLBZ (Verminator®, Elanco Animal Health/Eli Lilly Danmark A/S) at 1.43 mg/kg body weight and day. Chickens were euthanized and live worms at both time points were collected from the small intestine. Three independent biological replicates were collected on both occassions. The worms were washed extensively in phosphate buffered saline (PBS) (22°C) and then immediately submerged in RNA-later (Sigma-Aldricht), until they were individually transferred into Cryo-vials (Sigma-Aldricht) and frozen at -80°C. Total RNA was extracted and purified from the anterior end of A. galli worms using the Qiagen RNeasy Kit. RNA purification was performed using RNase-Free DNase Set (Qiagen). The libraries were prepared from 1µg total RNA using the TruSeq stranded mRNA sample preparation kit (Cat RS-122-2101/2102, Illumina Inc.) including polyA selection. The library preparation was performed according to the manufactors protocol (#15031047 and #15031048, rev E.)