16S RNA sequences of microbacteria in large intestine of PBS-administered mice # group B
C57BL/6 mice were administered with 200 microliters of PBS per os daily. After 30 days of administration, contents in large intestine from 3 mice were collected, pooled and homogenized. Genomic DNA was isolated from these intestinal contents. 16S rRNA gene fragments were then amplified using 27F and 519R primers and sequenced by barcoded-tag pyrosequencing.