GSM4391899: ATAC-Seq JHRCC12_SETD2_rescue_rep1; Homo sapiens; ATAC-seq
50,000 JHRCC12 cells were used for the transposition reaction at 37 °C for 30 min. After purification of the DNA with the MinElute PCR purification kit (Qiagen), material was amplified for 5 cycles as described previously (Buenrostro et al., 2013). Additional PCR cycles were evaluated by real time PCR. Final product was cleaned by AMPure Beads at a 1.5x ratio.