Human, NCT treatment for 24 h, replicate 1
Total RNA was extracted from HeLa cells with Rneasy_ Mini Kit (QIAGEN). Total RNA was poly-A purified by ambion Dynabeads mRNA DIRECT Micro Kit (Life Technologies). Poly-A purified RNA were used for RNA-seq library preparation using Ion Total RNAq Kit v2 (Life Technologies). Single-end sequencing was performed on Ion Proton System.