soil bacterial metagenome
An aliquot of the extracted DNA from each sample was used as a template for amplification.PCR reactions were performed in triplicate with a 20 l mixture containing 4 l of 5 FastPfu Buffer,2 l of 2.5 mM dNTPs, 0.8 l of each primer (5 M), 0.4 l of FastPfu Polymerase, and 10 ng of template DNA.