GSM2197698: WT mRNA P27-28 CB3; Mus musculus; RNA-Seq
For ChIP, lysates were purified from sonicated nuclei and histone-DNA complexes were isolated with the indicated antibodies. Total RNA was harvested with Trizol. For Sync-TRAP, ribosome-bound RNA were purified and amplified with Ovation RNA-Seq System V2 (NuGEN) ChIP-Seq libraries were prepared with the Illumina ChIP-Seq DNA Sample Prep Kit and RNA libraries were prepared for sequencing using standard Illumina protocols