Grape Phylloxera PacBio library
Four samples, each with ~ 600 adults of the Pcf7 clone, were extracted with a salting-out protocol 52 . Through this protocol a total of 120 um of long and ultrapure genomic DNA fragments were obtained. Quality was assessed with a NanoDrop (A260/280 ratio between 1.8 and 2.0 and A260/230 ratio 2.0 mm diameter) for 30s at 30 Hz (Tissuelyser, Retsch) and DNA was extracted using DNeasy Blood & Tissue kit (Qiagen Inc, Chatsworth, CA). Between 14-25 micro g rams of DNA were obtained from each sample after column elution with 100 microliters of 10 mM Tris-HCl-1 mM EDTA, pH 7.8. Quantitation of DNA was performed using DeNovix Fluorescence Assays.