LHMP Neat saline microbiota: bacterial16S rRNA gene sequences
PCR amplification of V5-V3 region of bacterial 16S rRNA-encoding gene using Primer A (CCATCTCATCCCTGCGTGTCTCCGACTCAGXXXXXCCGTCAATTCMTTTRAGTand Primer B (CCTATCCCCTGTGTGCCTTGGCAGTCTCAGCCTACGGGAGGCAGCAG) from HMP 16S Protocol Version 4.2 (http://www.hmpdacc.org/doc/16S_Sequencing_SOP_4.2.2.pdf). The region of Primer A represented by XXXXX is the 5-10 nucleotide barcode sequence. The remainder of Primer A and Primer B are the A adapter sequence and the B adapter sequence, respectively, required for emPCR and 454 sequencing.