GSM4704656: CUT&RUN IgG; Homo sapiens; OTHER
Total RNA was extracted from 5-AZA-CdR or mock-treated samples using QIAGEN RNeasy mini kit. DNA was extracted for CUT&RUN using QIAGEN MinElute Kit. For the MDA5 protection assay 100ng of RNA was used for the cDNA library construction using KAPA RNA Hyper-Prep with RiboErase library preparation kit from Roche. For the RNA sequencing on total RNA, libraries were prepared using TruSeq Stranded Total RNA Kit (Illumina) and 200ng of RNA underwent ribosomal RNA depletion using Ribo-zero Gold rRNA beads. For DNA-sequencing, libraries were prepared using NEB Ultra II library Preparation kit