snRNA-Seq of pooled moue cortex
Briefly, we thawed and minced tissue, dounced it in lysis buffer, filtered the lysate, and resuspended it in staining buffer. A brief incubation with Fc receptor blocking solution is followed by incubation with the TotalSeq Hashtag antibodies and 3 washes in ST-SB. Next, nuclei were counted and their concentration normalized to the desired loading concentration and pooled right before running the 10X Genomics single-cell 3 v2 assay (with minor adjustments listed in the detailed protocol), followed by library preparation and Illumina sequencing.