WGS of clonal suppressor ompB::tn R. parkeri strain
Rickettsia parkeri genomic DNA was isolated from frozen bacterial stocks using DNeasy Blood and Tissue kit (Qiagen) according to manufactures instruction for Gram-Negative bacteria. High molecular weight DNA was ensured through gel agarose electrophoresis. The genome of strains were sequenced using Single-end 50bp read lengths on the Illumina Hiseq2000 sequencing platform.