Illumina HiSeq 4000 paired end sequencing; Whole exome sequencing and transcription profiling of a patient cohort of oral cavity squamous cell carcinomas
Whole exome sequencing and transcription profiling of a patient cohort of oral cavity squamous cell carcinomas
Fresh tissue samples were collected and stored in RNAlater (Invitrogen, ThermoFisher Scientific) at -80°C. Acronyms used: MDSCC: moderately differentiated squamous-cell carcinoma; PDSCC: poorly differentiated squamous-cell carcinoma; WDSCC: well differentiated squamous-cell carcinoma; RTM: retromolar triangle; FOM: mouth floor RNA was extracted using the Qiagen RNeasy Mini Kit according to manufacturer's instructions. Low Throughput Illumina TruSeq RNA Sample Preparation Kit v2