GSM3406772: MapR_U87T_rep2; Homo sapiens; OTHER
DNA was collected from the supernatant after treatment with the indicated Mnase-containing fusion proteins as per the CUT&RUN protocol (Skene & Henikoff eLife 2017). DNA was end-repaired using End-It Repair Kit, tailed with an A using Klenow exo minus, and ligated to custom adapters with T4 DNA ligase. Fragments > 150 bp were size-selected with SPRI and subjected to ligation-mediated PCR amplification (LM-PCR) with custom barcoded adapters for Illumina sequencing using Q5 DNA polymerase. All enzymes except Q5 (NEB) were from Enzymatics (a Qiagen company).