GSM1357130: cph1/efg1_0min_4; Candida albicans; RNA-Seq
1x107 cells of the log-phase cultures were collected by centrifugation and frozen in liquid nitrogen until RNA extraction. After incubation under filament-inducing condition, medium and non-adherent cells were removed and 5 ml ice-cold PBS was added. The cells were collected by scraping and then centrifuged for 5 min at 6,000 rpm and 4°C. Cell pellets were snap frozen in liquid nitrogen. Total RNA was isolated using the Ribopure-Yeast Kit (Ambion) and treated with Turbo DNase (Ambion). RNA quality was determined in a Bioanalyzer with an RNA 6000 Nano LabChip Kit (Agilent Technologies) according to the manufacturer's protocol. RNA concentration was determined with a Nanodrop ND1000 (Peqlab) tagged random primed cDNA libraries were prepared from fragmented polyA-RNA using standard illumina protocols