GSM2971064: LPS630; Mus musculus; RNA-Seq
Mice were perfused intracardially with PBS. The cortex were dissected and homogenized in a glass-teflon dounce homogenizer. Leukocytes were prepurified using percoll gradient. Cells expressing jigh level of GFP were isolated by FACS. 5 ng of total RNA were amplified using the SMARTer ultra low RNA kit (Clontech) and 6 ng of amplified RNA wereused for  librairies construction  using the Ovation ultra low kit from Nugen