GSM3581248: T47D-TR-3; Homo sapiens; RNA-Seq
Cells were cllected on ice and RNA was extracted from each sample using TRIzol reagent according to the manufacturer's instruction. NEBNext ultra directional RNA Library Prep kit, NEBNext Poly (A) mRNA magnetic isolation module and NEBNext multiplex oligos (NEB, USA) was used with 1 ug of total RNA for the construction of sequencing libraries.