rARSDel-2_gen90_clone_4_Batch_Culture
Total cellular DNAs were isolated from stationary yeast cells by standard methodology.  DNA was fragmented by Covaris sonication (450 peak factor, 30% duty factor, 200 cycles per burst) prior to paired-index library construction using the KAPA Hyper Prep Kit.  Following qPCR for quality control and quantification, libraries were sequenced on an Illumina NextSeq 500 using the manufacturers recommended protocols.