GSM3272800: Hb_adult_monop_1; Heligmosomoides polygyrus bakeri; ncRNA-Seq
Total RNA was used directly for library preparation. Total RNA was extracted using a miRNAeasy mini kit (Qiagen) following manufacturer's instructions. RNA was treated with Turbo DNA-free kit (Thermo Fisher) to remove residual DNA. Libraries were prepared using the CleanTag small RNA library prep kit according to manufacturer's instructions, using total RNA from adult worms (30 ng), and sucrose-gradient purified EVs (equivalent 1E10 EVs measured by Nanosight, Malvern). For all samples, 1:12 dilutions of both adapters were used with 18 amplification cycles (TriLink BioTechnologies). Fragments between 140-170bp were size selected and sequenced on an Illumina HiSeq high output v4 50bp SE in Edinburgh Genomics.