RNA-Seq of Winthemia  rufopicta
One male specimen was collected by hand (USA: Ohio: Greene Co. Wright State University Woods. 5-July-2012. I.S. Winkler) and placed live in a collecting tube. The thorax from the live specimens was separated and crushed in RNAlater, and stored at 4C. RNA was isolated using a trizol/Chloroform extraction and purified using Qiagen RNeasy spin columns following manufacturers protocol: Purification of Total RNA from Animal Tissues (Qiagen 2006).mRNA samples were prepared using NEB RNASeq reagents utilized with an lab specific protocol (HudsonAlpha). Sequencing was conducted with a TruSeq PE v3 Cluster Kit and TruSeq PE v3 SBS reagents.