GSM5176456: GW15-A; Homo sapiens; RNA-Seq
Hypothalamic tissues were micro-dissected from serial coronal sections using a vibratome and dissociated into single-cell suspension using a papain-based dissociation protocol. Single cells were partitioned into nanoliter-scale Gel Bead-In-EMulsions (GEMs) by 10x™ GemCode™ Technology. The cDNA libraries were generated with Single Cell 3' Reagent Kits, according to the manufacturer's protocol. The library was processed on the Illumina HiSeq4000 platform for sequencing with 150 bp paired-end reads. Illumina HiSeq4000 platform