small RNA-seq of Drosophila melanogaster: spnE1/hls-03987; P{D.mel-spnE} adult ovary
3-6-day old female ovaries were dissected, placed directly in Trizol reagent (Invitrogen) and homogenized. Then, total RNAs were extracted according to the manufacturers instructions, and were size fractionated for 18-30nt small RNAs on 12% polyacrylamide/urea gel. Small RNAs were subsequently ligated to 3 and 5 adaptors, reverse transcribed and PCR amplified using NEBNext Multiplex Small RNA Library Prep Set for Illumina. Small RNA libraries were further purified from a 2% agarose gel and sequenced by Illumina platform.