RNA Seq V583 Uninduced 1
Bacteria were grown to OD600 of 05.-0.6 and were grown for an additional hour at 37 degrees C.  Bacterial cells were harvested by centrifugation, cell pellets were resuspended in trizol reagent and bacteria were lysed in a Beadbeater for 1 minute after the addition of 0.1 mm zirconia beads.  Following centrifugation, clarified lysates were applied to a ZymoResearch DirectZol column for RNA isolation.  Purified RNA was DNAse I-treated followed by removal of ribosomal RNA using the Epicentre RiboZero kit for Gram-positive bacteria.