Identification and characterization of a Rhodobacter capsulatus strain A12 with disease suppression and bioremediation function
Genomic DNA was extracted with the SDS method. Libraries for single-molecule real-time (SMRT) sequencing was constructed with aninsert size of 10 kb using the SMRT bell TM Template kit, version 1.0. Briefly, theprocess were that fragment and concentrate DNArepair DNA damage and endsprepare blunt ligation reactionpurify SMRTbell Templates with 0.45X AMPure PBBeadssize-selection using the BluePippin Systemrepair DNA damage after sizeselection. At last, the library quality was assessed on the Qubit 2.0 Fluorometer(Thermo Scientific) and detected the insert fragment size by Agilent 2100(AgilentTechnologies). The whole genome was sequenced using PacBio Sequel platform.