RNA-Seq for hydrotropic response in microtubules mutants
Total RNA was extracted from root samples using the TRIzol reagent (Invitrogen, Carlsbad, CA, USA). First-strand cDNA was generated using reverse transcriptase and random hexamers, and then sequenced using an Illumina HiSeq 2000 platform located at the Beijing Genomics Institute (Shenzhen, China).