GSM1845113: aub_sh_ago3_sh_totsRNAseq; Drosophila melanogaster; ncRNA-Seq
smallRNA cloning was performed according to Brennecke et al, Cell, 2007 small RNAs were PAGE selected and 3' and 5' linkers was ligated. 1st strand cDNA synthesis was performed using SuperScript III (Invitrogen) and the library was amplified using the Kapa HiFi Library Amp real time Kit (Peqlab) with Illumina TruSeq compatible primers. sizeselected 18-29nt smallRNA cloning