Sac_6
RNA samples with RIN values greater than 8 were processed for downstream library preparation and sequencing. Briefly, 300 ng total RNA per sample extracted from Tetracapsuloides bryosalmonae sacs, were used for library preparation with the TruSeq RNA Library Prep Kit v2 (Illumina) according to the manufacturers protocol. RNA-seq (paired-end 150-bp reads) was performed on llumina NextSeq550 platform.