RNA-Seq of Diporeia Hoyi:  Diporeia Hoyi Sample #121
Nucleic acids were extracted from individual amphipods via ZR-Duetª DNA/RNA MiniPrep kit.  RNA fractions were further enzymatically digested with TurboDNase for 15min to reduce co-extracted DNA. Ribosomal RNA was depleted via mRNA-ONLYª mRNA Isolation Kit, and remaining RNA was reverse transcribed and amplified via TransPlex¨ Complete Whole Transcriptome Amplification Kit. Resulting cDNA libraries were quantified via Pico Green fluorescence and prepared for sequencing using a Nextera XT DNA Library Preparation Kit. Resulting libraries were subjected to 2x250bp paired-end sequencing on an Illumina Miseq.