GSM5176156: MCF7_siRAC1_rep1; Homo sapiens; RNA-Seq
Total RNA was extracted after 3 days using TRIzol (Invitrogen). Ribosomal RNA was removed using the NEBNext rRNA Depletion kit (New England Biolabs #E6310). RNA library was prepared using NEBNext Ultra II Directional RNA Library Pep Kit (New England Biolabs #E7760).