ISVF-MDI
Cells from three individual swine were pooled, total RNA was extracted using TRIzol  Reagent (Invitrogen) according to the manufacturer  s instructions and then treated with DNase to remove potential genomic DNA contamination. Total RNA integrity and concentration were evaluated using an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, USA). The samples used had an average RIN (RNA integrity number)(RIN) value of 9.4 and a minimum RIN value of 9.1.