GSM3895948: T1: Roscovitine; Mus musculus; RNA-Seq
Retinas were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. RNA degradation and contamination were monitored on 1% agarose gels. RNA purity was checked using the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA). RNA concentration was measured using Qubit® RNA Assay Kit in Qubit® 2.0 Flurometer (Life Technologies, CA, USA). RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). RNA libraries were prepared for sequencing using standard Illumina protocols