UCE target enrichment of Andrena manifesta: whole organism
DNA extraction using CTAB buffer. Temperature-driven enzymatic fragmentation to ca. 350bp. Library preparation using NEBNext DNA Ultra II FS kit. UCE target enrichment and post-enrichment amplification using a UCE MyBaits Target Capture Kit. Sequencing on Illumina MiSeq using Reagent Kit v3 600-cycle MS-102-3003 with 5% phiX.