WGS of Pseudomonas aeruginosa:E-S2239-15
Genome assembly of this sample is available from bioproject - PRJNA325248, biosample - SAMN07424097 INFORMATION OBTAINED FROM; Freschi et al., (2019) The pseudomonas aeruginosa Pan-Genome provides new insights on its population structure, horizontal gene transfer and pathogenicity. Genome Biol Evol, 11(1). 109-120. - Bacterial colonies were isolated on Difco Pseudomonas Isolation Agar (BD, Sparks, MD). Genomic DNA was extracted from overnight cultures using the DNeasy Blood and Tissue Kit (QIAGEN, Hilden, Germany). Genomic DNA (500 ng) was mechanically fragmented for 40 s using a Covaris M220 (Covaris, Woburn, MA) with default settings. Fragmented DNA was transferred to a polymerase chain reaction tube and library synthesis was performed with the Kapa Hyperprep kit (Kapa Biosystems, Wilmington, MA) according to the manufacturers instructions. TruSeq HT adapters (Illumina, SanDiego, CA) were used to barcode the libraries, which were each sequenced in 1/48 of an Illumina MiSeq 300-bp paired-end run at the Plateforme dAnalyses Gnomiques of the Institut de Biologie Intgrative et des Systmes (Universit Laval, Qubec, Canada).