HeLa_Kyoto transcriptome sequencing 2
Cells were cultured in DMEM containing 4.5 g/l glucose, 10% heat inactivated foetal bovine serum, 2 mM glutamine, 100 U/ml penicillin and 100_g/ml streptomycin and incubated at 37C and 5% CO2 Total RNA was extracted using Trizol