GSM3272958: PFC_SALIp4; Mus musculus; RNA-Seq
RNA was isolated following the manufacturer's protocol and further purified using the RNeasy Mini Kit (Qiagen Inc.). The total RNA concentration was measured using an ND-1000 Spectrometer (NanoDrop Technologies Inc., Montchanin, DE, USA). rRNA-depleted RNA was used as the template to prepare the RNA-seq library generated using the Ion  123 Total RNA-seq Kit v2 system, according to the manufacturer's protocol. The template-positive ion sphere particles (ISPs) were loaded onto an Ion  127 PI Chip v2 and sequenced (single end reads > 100 bp).