RNA-Seq of Megacormus gertschi: pooled males and females
Automated DNA sequencing was performed at the Massive DNA Sequencing Facility in the Institute of Biotechnology (Cuernavaca, Mexico) with a Genome Analyzer IIx (Illumina), using a 72 bp paired-end sequencing scheme over cDNA fragments ranging in size of 200-400 bp. Each library consisted of two fastq files (forward and reverse reads), from which the adaptors were clipped-off. The quality of cleaned raw reads was assessed by means of the FastQC program