GSM3896440: Glia cells at P8 replicate 3 [Glia_P8_r3]; Mus musculus; RNA-Seq
Mouse cochlea or inner ear was dissected, digested with pronase for 30-45 mins at RT, triturated and tdtomato labeled cells were sorted manually. RNA was extracted using PicoPure kit (life technologies, KIT0204). Extracted RNA was amplified using Ovation RNA-Seq System V2 (NuGEN, #7102-32). 1.8~2.25 ug of the amplified material was used for library construction using Ovation Rapid DR multiplex system (NuGEN, #0319-32).