GSM3207752: SiHa/7.4 rep2; Homo sapiens; RNA-Seq
Total RNA from subconfluent cells (80-90% confluence) was extracted using Maxwell® RSC SimplyRNA tissue kit (Promega). RNA quantity and quality were evaluated with a Nanodrop 1000 spectrophotometer (Thermo Fisher Scientific) and RNA 6000 nano chips on a Bioanalyzer 2100 system (Agilent), respectively. Illumina TruSeq RNA Library Prep Kit V2 was used with 2 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols. RNA-Seq using the NextSeq 500 High Output Kit V2 (75 cycles; Illumina).