GSM3895606: Bcells_TENT5C_KO_1; Mus musculus; RNA-Seq
RNA isolation was performed with Trizol reagent (Invitrogen, 15596) according to the manufacturer's instructions and followed by ribodepletion using a Ribo-Zero Kit (Epicentre, RZG1224), according to the manufacturer's recommendations  and spike-in with external RNA (ERCC RNA Spike-In Mix, 4456740). Strand-specific libraries were prepared using a dUTP protocol (KAPA Stranded RNA-Seq Library Preparation Kit), according to the manufacturer. Library quality was assessed using chip electrophoresis performed on an Agilent 2100 Bioanalyzer (Agilent Technologies, Inc.).