GSM3788535: Control_3; Homo sapiens; RNA-Seq
Total RNA was isolated from Human Epidermal Keratinocytes by Qiazol lysis reagent (Qiagen, Hilden, Germany) and purified with miRNeasy mini kit (Qiagen, Hilden, Germany) following manufacturer´s instructions. RNA quantification was performed using NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific). Transcriptome libraries were then constructed using poly-A selection with the TruSeq Stranded mRNA Library Prep Kit (Cat. No. RS-122-2101, Illumina, San Diego, CA, USA). In brief, 300 ng of total RNA from each sample was used to construct a cDNA library, followed by sequencing on the Illumina HiSeq 2500 with single end 50 bp reads and ~30 millions of reads per sample.