GSM3407084: SMMC7721-shCtrl; Homo sapiens; RNA-Seq
Cells were washed three times with PBS before RNA was harvested using Trizol reagent. ΒioTeke RNA extraction Kit  (Cat#RP4002) was used with 1 ug of total RNA for the construction of sequencing libraries. For RNA-sequencing, ribosomal RNA was removed and strand-specifc sequencing libraries were generated using KAPA Riboerase-RNA Hyper Prep Kit following manufacture's recommendations. After proper quality control steps, the libraries were sequenced on illumina HiSeq platform