GSM3723011: Line 13 WT_Input; Homo sapiens; ChIP-Seq
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibodies to H3K27me3 (Cell Signaling C36B11) or H3.3  (Millipore #09-838). Libraries were prepared with the Nextera library prep kit Libraries were sequenced on the NovaSeq machine with 150 base paired-end sequencing