GSM5176502: PigGeCKO_Control; Sus scrofa; OTHER
Illumina sequencing of sgRNAs in the PigGeCKO cells. Briefly, the genomic DNA from PigGeCKO cells  were extracted using a Blood & Cell Culture DNA Midi Kit (QIAGEN), respectively. The sgRNA-coding region was amplified by PCR using Q5 Hot Start High-Fidelity DNA Polymerase (NEB). PCR products were purified.