Grape Phylloxera WGS  Paired ends reads (insert size=250bp)
genomic DNA was extracted from six samples of the Pcf7 clone, each corresponding to approximately 200 individuals, with a mix of adults and larvae. The insects were homogenized using three sterilised glass beads (2 mm diameter) for 30s at 30 Hz (Tissuelyser, Retsch) and DNA was extracted using DNeasy Blood & Tissue kit (Qiagen Inc, Chatsworth, CA). Between 14-25 micro g rams of DNA were obtained from each sample after column elution with 100 microliters of 10 mM Tris-HCl-1 mM EDTA, pH 7.8. Quantitation of DNA was performed using DeNovix Fluorescence Assays.