DNA METHYLATION PROFILING OF A CNIDARIAN-ALGAL SYMBIOSIS USING NANOPORE SEQUENCING
Frozen sea anemone tissue was ground to a fine powder with a mortar and pestle in liquid nitrogen prior to genomic DNA extraction with the Qiagen DNeasy Blood and Tissue kit. The Qubit BR assay was used to assess DNA quantity, followed by 1% agarose gel electrophoresis to confirm the presence of high molecular weight DNA (10 kb). Three libraries with one aposymbiotic individual and one symbiotic individual each, both individually barcoded, were prepared using the PCR-free ONT Ligation Sequencing Kit with the Native Barcoding Expansion Kit (SQK-LSK109 and EXP-NBD103), following the manufacturers 1D native barcoding gDNA protocol. Each library was run on a separate FLO-MIN 106D R9.4 flow cell.