Illumina HiSeq 2000 paired end sequencing; RNA-seq of wild type and mutant Drosophila flies with a deletion of the gene CG16717 (dMPPED)
RNA-seq of wild type and mutant Drosophila flies with a deletion of the gene CG16717 (dMPPED)
10 virgin wild type (w1118) and dMPPEDKO female flies (deletion in the gene CG16717) (35 days old) were anaesthetized and collected.   Flies (Drosophila melanogaster) were reared on standard media containing cornmeal, dextrose, yeast and agar along with antibacterial and antifungal agents. Cultures were maintained at 25 °C and 50% relative humidity under 12h light/12h dark cycles. The wild type strain used was w1118.  Flies allowed to grow in vials for 35 days with vial change every 3 days. RNA extraction was performed with the help of RNeasy kit  (Qiagen) as per manufacturer's protocol.  The RNA library was prepared as per NEBNext Ultra directional RNA library prep kit.