WGS Illumina for Serratia marcescens: Dryococelus australis
Genomic DNA extracted with High Pure PCR Template Preparation Kit (Roche), purified and concentrated in a final elution volume (30 L) with the DNA clean and concentrator kit (Zymo), and stored at -20C. Quality of genomic DNA preparations assessed by gel electrophoresis, hyperladder 1kb (Bioline) used to determine DNA fragment size and visually assess DNA integrity. Extracted DNA quantified using a Qubit 3.0 Fluorometer, RNA/DNA ratios checked using the Nanodrop (v3.8.1) spectrophotometer (Thermo Fisher Scientific). Genomic DNA quality testing, library preparation using the Nextera XT DNA library kit v3.0 and Illumina short read sequencing of the extracted DNA conducted in accordance with the manufacturers instructions by the Australian Genome Research Facility. Reads filtered for quality scores >20 and adapters removed using the wrapper Trim Galore v0.4.4.