GSM3790158: H2A.Z2 ChIP-seq in U2OS Flag-Z2 cells - Replicate #2; Homo sapiens; ChIP-Seq
Nuclei were isolated and chromatin was sonicated and precleared. The H2A.Z histone-DNA complexes were isolated using anti-Flag M2 antibody (Sigma). Spike-in chromatin and Spike-in antibody (Active motif) were added in the ChIP experiment. After purification, 10 ng of immunoprecipitated DNA was sent to EMBL Genecore (Heidelberg, Germany) for library construction and sequencing. Library construction (NEBNext® Ultra™ II DNA Library Prep Kit for Illumina) was performed by EMBL GeneCore. The 4 samples were barcoded and sequenced in one lane by NextSeq 500 Illumina device.