RNA-Seq of Limnobdella mexicana: salivary tissue
RNA was extracted using a standard TRIzol-based protocol. Quality and quantity of mRNA was measured with a pico RNA assay in an Agilent 21000 Bioanalyzer and quantity was also measured using a RNA assay on a Qubit fluorometer. cDNA was constructed on the Apollo 324 automated system using the PrepX mRNA kit, following the manufacturer protocol. Full-length cDNAs were fragmented to a mean insert size of 500 bp. Sequencing was performed in 1/6th of a lane on the Illumina HiSeq 2500 platform (paired end, 150 bp) at the FAS Center for Systems Biology at Harvard University, Cambridge, MA, USA.