GSM2970166: YP1_ChIPSeq; Oryza sativa Indica Group; ChIP-Seq
5 g < 0.5 cm (YP1) or < 2 cm (YP2) inflorescences were harvested and crosslinked with 1% (v/v) formaldehyde under vacuum for 30 min, then ground to powder in liquid nitrogen. The chromatin complexes were isolated, sonicated, and incubated with anti-GFP antibody (Abcam, ab290). The precipitated DNA was recovered and dissolved in 1×TE and stored at -80 °C for later in-depth analysis. Illumina sequencing libraries were constructed with the above-prepared DNA samples by ThruPLEX DNA-seq Kit (CAT. NO. R400406) mainly according to the manufacturer's instructions. Then, the library containing 270 bp to 330 bp fragments were purified and sequenced with Illumina Hiseq3000 system.