GSM4155627: ONAC127H-ChIP; Oryza sativa Japonica Group; ChIP-Seq
Caryopses at 7 DAP were harvested and immediately crosslinked in 1% formaldehyde under vacuum for 30 min and 2 g tissues for each sample were used for chromatin isolation. Chromatin was fragmented to 150-500 bp by sonication. ONAC127-DNA complexes and ONAC129-DNA complexes were isolated with FLAG magnetic beads, and the precipitated DNA was purified and then dissolved in ddH2O. The eluted DNA was uesd to generate Illumina sequencing libraries following the manufacture's protocol.