NextSeq 500 sequencing; Transcriptomics study on the effect of alternative oxidase expression in Bcs1l mutant (S78G) mice (Mus musculus)
Transcriptomics study on the effect of alternative oxidase expression in Bcs1l mutant (S78G) mice (Mus musculus)
For end point sacrificing and sample collection, the mice were not fasted for longer than 2-3 hours due to the hypoglycemia associated with the mutant phenotype. All samples were collected at the same time of the day during the light period of the mice. The mice were anesthetized with pentobarbital, blood was collected into Li-heparin tubes by cardiac puncture, and plasma was separated. Organs were immediately dissected and snap frozen in liquid nitrogen or immersed in 10% buffered formalin solution. Approximately 1 mm3 pieces of tissue were fixed in 1.5% glutaraldehyde, 1.5% paraformaldehyde in 0.1 M Sörensen buffer pH 7.2 for electron microscopy. Standard RNA isolation protocol for total RNA was used For RNA sequencing, rRNA was removed and the sequencing libraries were prepared using Illumina TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human/Mouse/Rat.