BGISEQ-500 sequencing; RNA-seq of liver from Ldlr-/- mouse fed with inulin or cellulose supplemented atherogenic diet
RNA-seq of liver from Ldlr-/- mouse fed with inulin or cellulose supplemented atherogenic diet
At the completion of week 12, animals were anesthetized using isoflurane prior to euthanasia. Tissues were harvested for mRNA and protein and all samples were stored at -80 °C prior to analysis. Mouse liver samples were homogenized and total RNA was extracted using the TRIzol reagent (Thermo Fisher Scientific Inc, Waltham, MA, USA) The quality of RNA was assessed using the Agilent 2100 Bioanalyzer system and three samples from each group with a RNA Integrity Number greater than 8 were used for RNA library construction.