GSM4391934: RNA-Seq JHRCC12_SETD2_mut_rep1; Homo sapiens; RNA-Seq
Total RNA was extracted and cleaned up using RNeasy Mini Kit (Qiagen). After RiboGreen quantification and quality control of Agilent BioAnalyzer, 6-15 ng of total RNA underwent amplification (12 cycles) using the SMART-seq V4 (Clontech) Ultra Low Input RNA kit for sequencing. 10 ng of amplified cDNA was used to prepare Illumina HiSeq libraries with the Kapa DNA library preparation chemistry (Kapa Biosystems) using 8 cycles of PCR.