Illumina HiSeq 2000 paired end sequencing; The ETS transcription factor ELF5 modulates estrogen action in breast cancer via participation in FOXA1-ER driven transcription.
The ETS transcription factor ELF5 modulates estrogen action in breast cancer via participation in FOXA1-ER driven transcription.
MCF7-ELF5-Isoform2-V5 cells treated with Dox or vehicle from 24 hours after plating for 48 hours.  RNA was extracted with phenol/chloroform using the miRNeasy Mini Kit (Qiagen) with on-column DNase treatment. RNA quality was assessed on the 2100 Bioanalyser using RNA Nano chips (Agilent), with all samples having an RNA Integrity Number (RIN) of 9.8-9.9 (out of 10).  Samples were prepared with the TruSeq Stranded Total RNA Sample prep kit (RS-122-2201 Illumina, San Diego, California, USA) according to the manufacturer's instructions. One ug of total RNA was used as input to the ribosomal ribozero RNA depletion, followed by 13 cycles of PCR to amplify the adapter-ligated cDNA.