16S rRNA of Staurosira sp. C323 microbiome - reused growth medium - Day 8
DNA was extracted with the Gentra Puregene Yeast/Bact. Kit (Qiagen 158567), using a modified protocol for gram-positive bacteria. PCR amplification used 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 926R (5-CCGYCAATTYMTTTRAGTTT) primers, targeting the V4 and V5 regions of the 16S rRNA gene. Triplicate PCR reactions per sample were pooled and amplified DNA was gel-purified. 5 ng of each sample was added to a pooled sample library. The library was submitted for Illumina MiSeq 2x 250 bp sequencing.