Illumina HiSeq 2500 paired end sequencing; ChIP-Seq of in Danio rerio 32hpf embryos for activator transcription factor 3 (atf3)
ChIP-Seq of in Danio rerio 32hpf embryos for activator transcription factor 3 (atf3)
ChIP assay was carried out within the HA-atf3 mRNA injected embryos at 32 hpf.  The sonicated DNA was precipitated by HA-Tag (C29F4, Cell Signalling) Rabbit mAb. Afterward, the precipitated DNA was collected by QIAquick PCR Purification Kit. 10 ng of DNA samples were prepared for Illumina sequencing as the standard steps using the TruSeq Rapid SBS Kit. Illumina's genomic adapters were ligated to the DNA fragments. Enriched ligated fragments of ~200 - 500 bp was purified and quantified. The libraries were denatured, captured on Illumina flow cell and amplified in situ.