GSM2536369: Sham-1; Homo sapiens; RNA-Seq
NHEKs were purified 6h after radiation using Qiagen Rneasy Plus kit according to manufacturer's instructions. RNA quality was assessed using Agilent Bioanalyzer. An oligo dT-selected library was prepared and sequencing was performed on an Illumina HiSeq2000 platform with 50 bp single-end reads by the Functional Genomics Facility at the University of Chicago using standard Illumina protocols.