GSM4154850: OM_036; Mus musculus; RNA-Seq
Fetal bone marrow and thymus samples were homogenised using a rotor-star homogeniser (Qiagen) and total RNA extracted via TRIzol (Ambion), followed by RNA elution using RNeasy MinElute (Qiagen). RNA integrity was determined using an Agilent 2100 Bioanalyzer (Agilent Technologies; RIN: 10 ± 0 (mean ± SD)). RNA libraries were prepared for sequencing using standard Illumina protocols.