GSM3356248: P14_1; Mus musculus; RNA-Seq
TissueLyser LT The mPFC region was isolated from 2, 6, 10, and 22 weeks old mice, and was homologized by TissueLyser LT (Qiagen, Germany). The total RNA was extracted using AllPrep DNA/RNA/miRNA Universal Kit (Qiagen, 80224) with QIAcube (Qiagen, Germany). 300 ng of total RNA extracted from mouse tissue was used for each library prep. RNAseq libraries were prepared using the TruSeq Stranded Total RNA Library Preparation kit with Ribo-Zero Gold ribosomal RNA depletion from Illumina (catalog number RS-122-2303) according to the manufacturer's instructions. The Invitrogen SuperScript III Reverse Transcriptase from ThermoFisher (catalog number 18080093) was used for cDNA synthesis.