Illumina ssRNA-Seq of Dioscorea alata breeding line TDa95/00328
Total RNA was extracted from TDa95/00328 plants grown at ICRAF in Nairobi, Kenya from leaf petiole, roots, initial sprouting leaf, leaf bud, young leaf, semi-matured leaf, matured leaf, fifth leaf, bark, stem, first internode, and middle vine using the Purelink RNA mini kit. Libraries were constructed with the Illumina TruSeq stranded mRNA sample preparation kit and sequenced on an Illumina HiSeq 2500 with v4 chemistry (2125 bp) at the Agricultural Research Council Biotechnology Platform (ARC-BTP) in Pretoria, South Africa.