Illumina HiSeq 2500 sequencing; RNA-seq of Th1 and Th2 differentiated T helper cells re-stimulated with pma/ionomycin after 7 days of differentiation.
RNA-seq of Th1 and Th2 differentiated T helper cells re-stimulated with pma/ionomycin after 7 days of differentiation.
CD4 T cells were collected by washing culture dish and washed twice by pbs. RNA was extracted using Norgen RNA isolation kit. Libraries were prepared using nebnext ultra RNA-seq kit and mRNA isolation module.