RNA-seq of zebrafish embryos: 36 hpf dmh4/+
20 embryos were pooled at 36 hpf, and RNA was extracted using the phenol-chloroform method. RNA sample quality was assessed using an Agilent Bioanalyzer. Library prep and subsequent sequencing was performed by The Centre for Applied Genomics at The Hospital for Sick Children (SickKids) using the NEBNext stranded RNA.