GSM5165386: C3_HF_P; Mus musculus; RNA-Seq
Fresh gonadal white adipose tissue was harvested after euthanasia and subjected to collagenase digestion. Tissue was centrifuged and filtered to separate the mature fraction from the stromal vascular fraction. Magnetic Activated Cell Sorting (MACs) was used to further isolate adipocytes from other cell types. Pre-and mature adipocytes were collected and saved in Purezol at -80oC for later RNA extraction using the Promega ReliaPrep RNA miniprep systems. Total RNA was sent to Novogene for RNAseq analysis. Novogene's RNA-Seq: Illumina Novaseq 6000, S4 flowcell, PE150 seq parameter.