GSM3895190: Dark-BCot_stranded_mRNAseq; Arabidopsis thaliana; ssRNA-seq
At the indicated time the roots were removed with a scalpel and two biological replicates of seedlings growing in the dark or moved to simulated white light were collected into ice cold 80% acetone. Dark samples were collected under safe green light The cotyledons and the hypocotyl were separated under a stereomicroscope as described in (Park et al., 2012) RNA was extracted with RNeasy Micro Kit (Qiagen cat. 74004) 500ng of total RNA was used to prepare RNA-seq libraries, using TruSeq Library prep kit-v2 (Illumina).