RNA-seq from wild Metrosideros nervulosa and M. sclerocarpa
Mature leaf tissue was collected for ten adult individuals each of Metrosiderosnervulosa and M. sclerocarpa between 12th and 14th of April 2016. Tissuewas immediately dissected into 5mm sections and stored in RNAlater (Sigma) at -20C. Leaf tissue samples were sent to the BGI Tech Solutions (Hong Kong) forRNA extraction, Illumina library preparation, and paired-end sequencing. Paired-end100 base pairs (bp) libraries were multiplexed and sequenced on an Illumina HiSeq4000 sequencer. For each species, 9 individuals were sequence to a depth of >10million read pairs. A single individual of M. nervulosa and M. sclerocarpa wassequenced to a depth of > 40 million read pairs to be used as reference fortranscriptome assembly