GSM4103442: afla271: AF LA biological replicate 2; Homo sapiens; RNA-Seq
Human heart tissue was flash frozen using liquid nitrogen. RNA was extracted from PCM1+ cardiomyocytes nuclei using Zymo Direct-zol RNA MicroPrep kit(Cat# 11-330M) and submitted to the Genome Technology Access Center (GTAC) at Washington University School of Medicine (St. Louis, MO) for RNA sequencing. Indexed RNA libraries were prepared from 10 ng of RNA from each sample with the Sigma Seqplex RNA amplification Kit (Sigma, Cat# SEQR). cDNA libraries were PCR-amplified in the presence of indexing adapters and pooled for RNA-sequencing. To minimize batch effects, library preparations for samples from each individual heart were performed simultaneously. Single-end 1 × 50 sequencing was performed at the on an Illumina HiSeq 2500 sequencer.