GSM1843531: Wild-type Villus H3K4me3 ChIP-Seq; Mus musculus; ChIP-Seq
Epithelium from proximal 1/3 of mouse intestine was isolated by incubation with 5mM EDTA in PBS for 45 minutes at 4°C. Cells were snap-frozen in liquid nitrogen and pooled for subsequent ChIP analysis. Villus cell pallet was digested with MNase (Sigma N3755) and dialised against RIPA buffer. The digested chromatin was incubated with 5ug of H3K4me3 antibody overnight (Abcam ab8580) for immunoprecipitation. The ThruPLEX DNA-seq Kit (Rubicon Genomics) was used to prepare libraries, and 75 bp single-end reads were sequenced on an Illumina NextSeq 500 instrument.