GSM4396378: wounded_1_10x; Drosophila melanogaster; RNA-Seq
Larvae were bled and the hemocytes were were encapsulated at the Single Cell Core from ICCB-Longwood screening facility with inDrops (Zilionis et al., 2017), 10x Genomics, or Drop-seq technologies. For inDrops samples: Reverse transcription and library preparation were done at the same facility as previously described (Zilionis et al., 2017). 10x cells were prepared following the manufacturer's protocol. All the Drop-seq and cDNA synthesis methods followed a previous study (Macosko et al., 2015).