Archaeal West Point WWTP (Seattle, USA) granular sludge community
Microbial samples were taken at regular intervals and frozen at -80C. At the end of the experiment, all samples were sequenced using bacteria and archaea V1-3 16s rRNA primer sets as per the MiDAS standard protocols version 1.2 and 1.0, respectively. DNA extraction was done with the DNeasy PowerWater Kit (Qiagen, Germany). Community sequencing was carried out by Novogene (China) using the NovaSeq 6000 platform (Illumina, USA).