GSM1200337: s24_5h; Prochlorococcus marinus subsp. pastoris str. CCMP1986; RNA-Seq
Before RNA extraction, cells were treated with 150 ml 10 mM Tris-HCl (pH 7.5), 2 ml RNase inhibitor (20 u/uL, AM2696), and 1 ml Readylysis lysozyme (EPICENTRE, Cat#R1810). Total RNA extraction was extracted by using the mirVana miRNA kit (Ambion). DNA was removed using Turbo DNA-free™ Kit (Ambion) RNA libraries were prepared for sequencing using standard Illumina protocols