GSM3580668: 275_lact1; Stenotrophomonas maltophilia; RNA-Seq
Cells were collected via centrifugation and immediately resuspended in 800 µl of ~80 C RNAzol. Total RNA was then extracted from these samples using Zymo Research's RNA Miniprep Kit. Residual genomic DNA was removed from each sample through DNaseI treatment (NEB), and the RNA was re-purified using Qiagen's RNeasy kit. RNA was fragmented using the NEBNext Magnesium RNA Fragmentation Module, with a 3 minute incubation at 94°C, immediately followed by a cleanup step using the RNA Clean & Concentrator-5 (Zymo Research). Double-stranded, tagged cDNA was generated from 50 ng of fragmented RNA by the Peregrine method. RNA-seq was performed in biological duplicate for each strain and each condition