transcriptome of Liver and Gill of M. amblycephala
Blunt snout bream (Megalobrama amblycephala) is a hypoxia-sensitive freshwater fish species. Low oxygen usually causes serious economic losses during aquaculture. Using Illumina Hiseq™ 2000, RNA sequencing of the 8 samples (liver and gill of group FH, SH, FN, SN) produced a total of 185 million high quality reads from the normalized cDNA of pooled blunt snout bream individuals. These sequences were assembled into 465,582 contigs and 237,172 transcripts. After BLAST searches against the NCBI, 31,338 unigenes were functionally annotated and classified. A comparative genomics approach revealed a substantial proportion of genes expressed in blunt snout bream tanscriptome to be shared across the genomes of zebrafish, medaka, tetraodon, fugu, stickleback, human, mouse, and chicken, and identified a substantial number of potentially novel blunt snout bream genes.