GSM2533719: BU3_C57_B6; Homo sapiens; RNA-Seq
Single cells enriched by FACS were captured by C1 Single-cell Auto Prep System(Fluidigm).  polyA RNA from each single cell was reverse transcribed and cDNA amplified using SMARTer kit (SMARTer Ultra Low RNA Kit for the Fluidigm C1 System,Clontech#634936) 0.25 ng of amplified cDNA was used as input for the NexteraXT kit (Illumina #FC-131-1096) and indexed with dual indices (Illumina #FC-131-2001)