WGS of Rhipicephalus appendiculatus: semi-engorged adult female
DNA was isolated from dried whole tick using DNA Isolate II kit (Bioline, Australia) according to manufacturers instructions and eluted in 100 ul of Tris buffer (pH = 8.5). DNA was stored at -20 C. Isolated DNA was used for NEBNext DNA Library Prep Kit construction followed by the next generation sequencing using 150bp paired end Illumina sequencing systems utilizing at the depth of 1Gb of raw sequence data (Novogene, Sigapore).