GSM4705238: HEK293T_mNG_posctrl_polyA; Homo sapiens; RNA-Seq
RNA was extracted with NucleoZOL (Macherey-Nagel) RNA was poly-A selected with oligo dT-beads (Dynabeads® mRNA DIRECT™ Kit life tech). For the control constructs, poly-A selected libraries were prepared. Poly-A RNA from oligo-pool transfected cells was reverse transcribed to obtain the constant or variable fragments of the constructs with the matching 8 nt barcode. PCR amplicons of these fragments were generated.