16S rRNA of bacteria: bottom waters in the methane seeps at SCS
The bacterial 16S rRNA gene V3V4 hypervariable region was amplified with the specific forward primer 314F 5-CCTAYGGGRBGCASCAG-3and the reverse primer 806R 5- GGACTACNNGGGTATCTAAT-3. 16S rRNA sequencing library construction were conducted according to the manufacturers protocols of NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, USA).