RNA seq of Euphyllia paradivisa: 280C symbiont 3
Samples of 1.5 μg RNA (n=36, including 3 repeats from each treatment) were prepared using paired end 300bp reads from a single Illumina MiSeq run. Overall, 36 libraries of the four sets (symbiotic, apo-symbiotic, “Sterile” symbiotic and apo-symbiotic polyps) at 3 temperature treatments were run on three lanes of Illumina HiSeq2000 using the multiplexing strategy of the TruSeq protocol. On average, ~10 million paired-end reads were obtained for each sample. The sequencing was performed at the NGS unit of the Technion research and development foundation LTD (http://isu.thecnion.ac.il), Israel.