U87_nanopore_1
Total RNA was isolated from U87 human glioblastoma cells using TRI Reagent Solution (ThermoFisher), followed by bead-based poly(A) selection. Approximately 750 ng of poly(A) RNA was used for dT adapter ligation, followed by reverse transcription, and additional ligation of motor adapter prior to loading onto the Oxford Nanopore Technologies (ONT) PromethION for sequencing