Transcriptome profiles of alternative male reproductive tactics in bluegill sunfish
Libraries were constructed using Illumina TrueSeq Stranded mRNA Library Preparation Kits LT, with each experimental individual receiving a uniquely identifiable index tag. The quality of each library was evaluated and the individuals were multiplexed into a single sample that was run on two lanes of an Illumina HiSeq 2500 Rapid Run Flow Cell (v1). Sequencing was performed on 2 x 150 bp format reads.