GSM5221240: IMCD_CRPH_7; Homo sapiens; RNA-Seq
Biopsies were obtained during endoscopies, and they were stored in RNA-later at 4 °C overnight and at -80 °C for long-term storage. Total RNA was extracted from cell lysates by grinding the tissues with an abrasive rod to augment cell disruption. RNA was prepared as instructed, and the integrity of RNA was assessed using agarose gel electrophoresis RNA libraries were prepared for sequencing using standard Illumina protocols