GSM5076863: 0 mM VPA for 5 days, sample 1; Homo sapiens; RNA-Seq
Panc-1-cell RNA was isolated using a RNeasy Mini kit (Qiagen). Total RNA concentration and purity was determined using a NanoDrop 1000 Spectrophotometer (NanoDrop) at a sample absorbance of 260/280 nm. To avoid genomic DNA contaminaton, samples were treated by DNase I (Qiagen). Total RNA and library integrity were verified with LabChip Gx Touch 24 (Perkin Elmer). 1 μg of total RNA was used as input 81 for SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Clontech) following standard 82 instructions.