Eptatretus cirrhatus (New Zealand Hagfish) Eye Sample 5
A barcoded cDNA library was prepared from each sample of total RNA.  The kit and protocol used were the Illumina TruSeq RNA Sample Preparation kit v2 (catalog # RS-122-2001) and the TruSeq RNA Sample Preparation v2 Guide (part # 15026495).  Using 1 µg of sample, the RNA was purified and fragmented, followed by first strand cDNA synthesis, second strand synthesis, adenylate 3' ends, adaptor ligation, and PCR amplification.  Library validation was done using the Agilent 2100 Bioanalyzer High Sensitive DNA Chip (part # 5067 4626).