Effect of temperature on template-switching reactions using oligonucleotides ending with three DNA, RNA or LNA bases.
The structures of NCms10013 are  - 6 bases of barcode 1 ;  - 8 bases of random sequence;  - 4 bases of spacer (TATA);  - 3 bases of linker (GGG);  - the remaining bases are the CAGE tags.and - 6 bases of index.The corresponds between sample name and barcode 1 and 6base of index are below.NC2lg_#36 (37C_index1) ... CACTGA , ATCACGNC2lg_#69 (37C_index1) ... GCTCTC , ATCACGNC2lg_#95 (37C_index1) ... TCGCGT , ATCACGNC2lg_dna_#24 (37C_index1) ... ATCGTG , ATCACGNC2lg_dna_#31 (37C_index1) ... CACGAT , ATCACGNC2lg_dna_#79 (37C_index1) ... GTATAC , ATCACGNC2lg_dnaL3_#21 (37C_index1) ... ATCATA , ATCACGNC2lg_dnaL3_#45 (37C_index1) ... CGATGA , ATCACGNC2lg_dnaL3_#83 (37C_index1) ... TATAGC , ATCACGNC2lg_#36 (42C_index2) ... CACTGA , CGATGTNC2lg_#69 (42C_index2) ... GCTCTC , CGATGTNC2lg_#95 (42C_index2) ... TCGCGT , CGATGTNC2lg_dna_#24 (42C_index2) ... ATCGTG , CGATGTNC2lg_dna_#31 (42C_index2) ... CACGAT , CGATGTNC2lg_dna_#79 (42C_index2) ... GTATAC , CGATGTNC2lg_dnaL3_#21 (42C_index2) ... ATCATA , CGATGTNC2lg_dnaL3_#45 (42C_index2) ... CGATGA , CGATGTNC2lg_dnaL3_#83 (42C_index2) ... TATAGC , CGATGTNC2lg_#36 (47C_index3) ... CACTGA , TTAGGCNC2lg_#69 (47C_index3) ... GCTCTC , TTAGGCNC2lg_#95 (47C_index3) ... TCGCGT , TTAGGCNC2lg_dna_#24 (47C_index3) ... ATCGTG , TTAGGCNC2lg_dna_#31 (47C_index3) ... CACGAT , TTAGGCNC2lg_dna_#79 (47C_index3) ... GTATAC , TTAGGCNC2lg_dnaL3_#21 (47C_index3) ... ATCATA , TTAGGCNC2lg_dnaL3_#45 (47C_index3) ... CGATGA , TTAGGCNC2lg_dnaL3_#83 (47C_index3) ... TATAGC , TTAGGCNC2lg_#36 (52C_index4) ... CACTGA , TGACCANC2lg_#69 (52C_index4) ... GCTCTC , TGACCANC2lg_#95 (52C_index4) ... TCGCGT , TGACCANC2lg_dna_#24 (52C_index4) ... ATCGTG , TGACCANC2lg_dna_#31 (52C_index4) ... CACGAT , TGACCANC2lg_dna_#79 (52C_index4) ... GTATAC , TGACCANC2lg_dnaL3_#21 (52C_index4) ... ATCATA , TGACCANC2lg_dnaL3_#45 (52C_index4) ... CGATGA , TGACCANC2lg_dnaL3_#83 (52C_index4) ... TATAGC , TGACCANC2lg_#36 (57C_index5) ... CACTGA , ACAGTGNC2lg_#69 (57C_index5) ... GCTCTC , ACAGTGNC2lg_#95 (57C_index5) ... TCGCGT , ACAGTGNC2lg_dna_#24 (57C_index5) ... ATCGTG , ACAGTGNC2lg_dna_#31 (57C_index5) ... CACGAT , ACAGTGNC2lg_dna_#79 (57C_index5) ... GTATAC , ACAGTGNC2lg_dnaL3_#21 (57C_index5) ... ATCATA , ACAGTGNC2lg_dnaL3_#45 (57C_index5) ... CGATGA , ACAGTGNC2lg_dnaL3_#83 (57C_index5) ... TATAGC , ACAGTG