GSM4704357: RNAiD - 3fold [93432]; Caenorhabditis elegans; miRNA-Seq
Total RNA was extracted from embryo, L1 larvae, or arabidposis flowers collected in Trizol using a phenol-chloroform-based standard protocol. Small RNA sequencing libraries were constructed from an input of 25-75 embryos, corresponding to an estimated amount of 10-20 ng total RNA RNA libraries were constructed as described in Alberti et al., 2018, Nature Methods. Given the low RNA input the protocol was modified: Less 5'- and 3' linker were used (500 nM final reaction concentration)