GSM3354869: RNASeq_CD3_LN809; Mus musculus; RNA-Seq
For RNA-seq cells were sorted based on the following surface markers: ProB LIN-(Gr1, Ter119, CD11b, CD11c, CD3, NK1.1)CD19+IgM-IgD-CD43+KIT+, MSC LIN-(Gr1, CD11b, CD19, CD11c, CD3, NK1.1, B220)Ter119-CD31-CD44-CD51+SCA1+, MPC LIN-(Gr1, CD11b, CD19, CD11c, CD3, NK1.1, B220)TER119-CD31-CD44-CD51+SCA1-, CD51/SCA1 LIN-(Gr1, CD11b, CD19, CD11c, CD3, NK1.1, B220)TER119-CD31-CD44-CD51-SCA1-, CD44+ LIN-(Gr1, CD11b, CD19, CD11c, CD3, NK1.1, B220)Ter119-CD31-CD44+.Total RNA was isolated by use of RNAeasy Micro Kit (Qiagen, Hilden, Germany) according to  manufacturer's recommendations. RNA-seq: Libraries were constructed using NuGEN's Ovation Ultralow Library systems (NuGEN Technologies, San Calros, CA) and were subsequently subjected to 76 cycles of NextSeq500 sequencing (Illumina, San Diego,CA).