GSM1357914: TK_wt_exp_plus_TEX; Thermococcus kodakarensis KOD1; RNA-Seq
RNA was extracted using TRIzol (Invitrogen) according to the manufacturer Total RNA was freed of residual genomic DNA by DNase I treatment. For depletion of processed transcripts, equal amounts of T. kodakarensis RNA were incubated with TerminatorTM 5'-phosphate-dependent exonuclease (TEX) (Epicentre #TER51020) as previously described (Sharma et al., 2010).  Libraries for Solexa sequencing (HiSeq) of cDNA were constructed by vertis Biotechnology AG, Germany (http://www.vertis-biotech.com/), as described previously for eukaryotic microRNA (Berezikov et al., 2006) but omitting the RNA size-fractionation step prior to cDNA synthesis