Illumina HiSeq 2000 paired end sequencing; RNA sequencing of the treatment of Aspergillus fumigatus with the new antifungal drug 089 -SYBARIS
RNA sequencing of the treatment of Aspergillus fumigatus with the new antifungal drug 089 -SYBARIS
A. fumigatus conidia were collected from solid Saboraud cultures using PBS+0.1% Tween, avoiding mycelium fragment and hyphae collection.  Conidia were then inoculated in RPMI 1640 medium + 2% glucose at 2.5x105 conidia/ml concentration supplemented with either 0.1mM 089 molecule or an equal volume of DMSO.  Cells were grown for 4 hours in static at 37°C. After 4 hours of growth in static at 37°C, RNA was extracted from flash frozen samples by bead beating with Trizol. For each sample, one paired-end mRNA library with approximately 150bp insert size was prepared using the TruSeq RNA Sample Preparation Kit v2 (Illumina).