GSM4102023: Cnot3-T292A/S294A rep3 [CD3_S54]; Mus musculus; RNA-Seq
Cells were collected and counted. RNA was extracted from 2 million cells using Trizol (ThermoFisher), 2 ul of a 1:10 dilution of ERCC Spike-in Mix (ThermoFisher) were added to each sample. 500 ng of RNA were used to prepare a polyA RNA library using the TruSeq Stranded mRNA Library Prep Kit (Illumina)