DNA-seq of Staphylococcus sciuri
Extraction of genomic DNA (gDNA) was performed with a commercial DNA extraction kit MagAttract HMW DNA Kit (Qiagen, Hilden, Germany), according to the manufacturers instructions. gDNA was quantified on a Qubit 2.0 Fluorometer using the dsDNA BR Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturers instructions and diluted to 0.2 ng/l as recommended by the manufacturer (Illumina sample preparation guide). Nextera XT Kit (Illumina) was used for preparation of ready-to-sequence libraries of bacterial genomes. MiSeq Reagent Kit v3 containing the reagent cartridge and the flow cell were used for sequencing on an Illumina Miseq. Sequencing was performed in both directions (paired-end sequencing) using a read length of 2 x 300 bp.