16S rRNA: internal microbiome of Myrmica scabrinodis
The bacterial V5-V6 domain of 16S rRNA was PCR amplied using 799F (CMGGGTATCTAATCCKGTT) and 1115R (AGGGTTGCGCTCGTTG) indexed primers. We used a dual barcoding approach with two primer sets to build the Illumina sequencing construct, as in Kembel et al. (2014) and McFrederick and Rehan (2016).