GSM2970225: CLP exvivo rep1; Mus musculus; RNA-Seq
RNA was extracted by TRIZOL reagent (Thermo Fischer Scientific) from 1x105 cells of FACS-sorted Lin-IL7R+Flk2+CD27+Ly6d- cCLP cultured in vitro for 2 months, or from freshly isolated CLP ex vivo. The TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero (RS-122-2201, Illumina) was used to prepare cDNA libraries from 100 ng total RNA according to the low sample protocol (RS-122-9007DOC Part #15031048 Rev. E). The resulting cDNA libraries were paired-end (2 x75 nt) sequenced on a NextSeq500 Illumina device using the NextSeq 500/550 Mid output Kit v2 with 150 cycles.