RNA-Seq of Anahita punctulata male: venom gland
Whole RNA was isolated from the venom glands using TRIzol (Life Technologies, Carlsbad, CA) and the Qiagen RNeasy kit (Qiagen, Valencia, CA). RNA concentration and integrity was evaluated using Qubit picogreen and Bioanalyzer. The RNA extraction was sent to the Genomic Services Lab at HudsonAlpha (Huntsville, AL) for library preparation with poly(A) selection and sequencing.