GSM5176198: 48-1; Mus musculus; RNA-Seq
RNA was isolated (gfapCre+ EAAT2Δ/+, n = 6; gfap-Cre− EAAT2+/+, n = 6; syn-Cre+ EAAT2−/−, n = 6; syn-Cre− EAAT2+/+, n = 6 mice) using a RNeasy Lipid Tissue Mini Kit (Qiagen). Quality of RNA samples was determined using a bioanalyzer (Agilent). Samples for RNA-Seq with RNA integrity number values >9. 500 ng of purified RNA were used to prepare sequencing libraries according to the Illumina protocol and sequenced with an Illumina HiSeq 2500 system.