GSM4395920: Liver_Epithelium_LCMVd8_RNA_3; Mus musculus; RNA-Seq
Organs were digested with Accumax and structural cells isolated using FACS For each individual assay, a maximum of 200 cells were sort-purified and deposited in 96-well plates con-taining 4 μl lysis buffer (1:20 solution of RNase Inhibitor (Clontech) in 0.2% v/v Triton X-100 (Sigma-Aldrich)), spun down, and immediately frozen at -80ºC. Reverse transcription and PCR were performed as described (Picelli et al., 2014). Library preparation was conducted on 1 ng of cDNA using the Nextera XT DNA Sample Preparation Kit (Illumina) followed by SPRI (Beckman Coulter) size selection. Sequencing was performed by the Biomedical Sequencing Facility at CeMM using the Illumina HiSeq 3000/4000 platform and the 50-bp single-end configuration.