NextSeq 500 paired end sequencing; RNAseq of the spleen of Hbb(th3/+) mice transplanted with bone marrow from Tfr2-ko or Tfr2 wt mice
RNAseq of the spleen of Hbb(th3/+) mice transplanted with bone marrow from Tfr2-ko or Tfr2 wt mice
C57Bl/6 Ly5.1 male mice lethally irradiated have been transplanted at 8 weeks of age with bone marrow from Hbb(th3/+)/Tfr2(+/+) or Hbb(th3/+)/Tfr2(-/-) mice (Bone marrow in a mixed genetic background C57BL6/129Sv). After transplantation, the mice have been maintained in a standard diet for 22 weeks and euthanised for organs extraction and analysis. Mice were euthanized 22 weeks after BM transplant and the spleen was extracted and SNAP-frozen. total RNA has been extracted with Trizol mRNA-Seq libraries were created according to the Truseq stranded mRNA Illumina protocol.