Illumina Genome Analyzer IIx sequencing; Transcriptome analysis of melon-Fusarium oxysporum f. sp. melonis race 1.2 pathosystem using RNA-Sequencing
Transcriptome analysis of melon-Fusarium oxysporum f. sp. melonis race 1.2 pathosystem using RNA-Sequencing
"sample growt protocol: melon seedlings were cultivated in plastic pots filled with sterilized soil in the greenhouse at 25±2 °C with 80-90% relative humidity. The FOM1.2 strain ISPaVe1018 was obtained from the fungal collection of the \""Centro di ricerca per la patologia vegetale\"" (CREA-PAV, Rome). The inoculum preparation and artificial inoculation procedures were reported in Sestili et al. (2011). After root trimming, the plantlets were dipped for 30 min in the conidial suspension. Mock-inoculations were performed in sterile distilled water. Seedlings were transferred into plastic pots filled with sterilized soil and maintained in the greenhouse at 25±2 °C with 80-90% relative humidity. Stems of ten plants per each time point (24 and 48 hpi) were pooled and used for the transcriptome analysis. In addition, 50 plants for each genotype were phenotypically screened at 8, 15, 21 and 35 dpi." Total RNA was isolated from melon infected and mock-treated plants at 24 and 48 hpi using the TRIzol reagent (Invitrogen) and purified by the Qiagen RNeasy® Minikit (Qiagen, Valencia, CA, USA; http://www.qiagen.com), according to manufacturer’s instructions. Two biological replicates for each genotype and treatment were used according to recommended RNA-Seq standards.