GSM2627287: Young rep5; Mus musculus; RNA-Seq
Total RNA was extracted using the Allprep DNA/RNA Mini kit (Qiagen 80204) followed by DNase treatment using the TURBO DNA-free kit (Life Technologies AM1907) mRNA was isolated from total DNA-free RNA (350ng) using the Dynabeads mRNA purification kit (Life Technologies 61006). Indexed, strand specific libraries were prepared using the ScriptSeq v2 RNA-Seq Library Preparation kit (Epicentre SSV21106) according to the manufacturer's instructions. Libraries were quantified/assessed using both the KAPA Library Quantification Kit (KAPA Biosystems KK4824) and Bioanalyzer 2100 system (Agilent). Indexed libraries were pooled and sequenced with a 100bp single-end protocol on an Illumina HiSeq2500 sequencer.