Continuos-flow culture of tilapia intestinal content at day 3
DNA was extracted from the farm (intestinal contents, J and F) and day 0, following 24-hour incubation of the combined intestinal contents from Nile tiapia and at 1, 2, 3, 5, 17, and 33 days incubation of the CFCEC using the Stool DNA Isolation kit (Norgen, Thorold, ON, Canada) according to the manufacturers instructions. PCR primers were used to sequence the highly variable V3-V4 region (farm samples) and V4 region (CFCEC samples) of the 16S rRNA gene.The PCR products were sequenced at Molecular Research DNA Lab (Shallowater, TX, USA) using 300 to 570 bp paired-end sequencing with an Illumina Miseq following the manufacturer's guidelines.