Metagenomic sequencing of artificial community derived from Homo sapiens stool treated with PEA
DNA samples were quantified by Quant-iT PicoGreen dsDNA Assay (Life Technologies) and normalized to a concentration of 50pg/uL.  Libraries were prepared from 100-250pg of DNA using the Nextera XT DNA Library Preparation kit (Illumina) according to the manufacturers protocol, with reaction volumes scaled accordingly. Insert sizes and concentrations for each library pool of 96 samples were determined using an Agilent Bioanalyzer DNA 1000 kit (Agilent Technologies). Libraries were sequenced at the Broad Institute Genomics Platform on a HiSeq 2500 with 101 bp paired-end reads.