Illumina MiSeq of 16S rRNA amplions from cave subaerial biofilms: RN5 site 6
Nucleic acids were extracted from scrapings of cave-wall biofilm, done using a sterile inoculating loop, and the V4 region of the 16S rRNA gene was amplified by PCR (primers 515F/806R) and sequenced on a MiSeq Illumina platform.