GSM5076467: b066: deplete_L_T0 A; Dokdonia sp. Dokd-P16; RNA-Seq
Total RNA from filtered cultured samples was extracted using RNeasy PowerWater kit (Qiagen, Hilden, Germany) following the manufacturer's instructions. The integrity of RNA samples was assessed using Bio-analyzer 2100 with the Agilent RNA 6000 Pico Kit (Agilent Technologies). The concentration of RNA samples was measured using the Qubit HS RNA kit on the Qubit 4. Extracted total RNA samples were sent to genome Quebec, Canada (https://cesgq.com/home) for rRNA depletion, library preparation and sequencing. For RNA-seq analyses, rRNA was removed by Ribo-Zero rRNA Removal kit (Illumina). After depletion of rRNA, RNA-seq libraries were generated with NEBNext UltraII directional RNA library prep kit for Illumina (New England Biolabs) following the manufacturer's instructions. The libraries were sequenced with Illumina Novaseq to generate paired-ends 100 bp reads.