Sugar cane vinasse fermented 2
The universal primers 520f and 802r targeting the V4 region of the 16S rRNA gene for bacteria, were used. The PCR was performed in a single-step 30 cycle PCR under the following conditions: 94C for 5 min, followed by 30 cycles of 94C for 30 sec, 55C for 30 sec and 72C for 1.5 min, then a final elongation step at 72C for 10 min was performed. The expected-size PCR products were purified on 1.8% agarose gel. Adapters and barcordes for Ion Torrent sequencing were included in the primers following a previously strategy. Sequencing of the 16S rRNA gene amplicon libraries was carried out on the Ion Torrent PGM.