GSM3273124: PI2; Solanum lycopersicum; RNA-Seq
Generative cells and sperm cells were isolated from just-germinated pollen cultured in vitro for 20 min and 10-h-cultured pollen tubes(Lu et al., 2015), respectively. For microspore isolation, flowers 7 mm long were excised and anthers 5 mm long were squashed in ice-cold 0.6 M mannitol solution. The released microspore was filtered through a 75-μm nylon mesh and collected by centrifugation at 200 g for 2 min. Strand-specific sequencing libraries were generated by using the rRNA-depleted RNA and NEBNext Ultra directional RNA library prep kit for Illumina (NEB) following the manufacturer's recommendations. The libraries were qualified on the Agilent Bioanalyzer 2100 system, sequenced on an Illumina Hiseq 2000 platform, and 100-bp paired-end reads were generated.