GSM1843501: 390_EtOH_rep1; Saccharomyces cerevisiae; RNA-Seq
Cultures were mixed quickly with cold methanol. After >1hr in cold methanol, cells were collected by centrifuging at 4 degree C and washed with ice cold water. Prior to performing standard RNA extraction protocols (with on-column DNase digestion) with RNeasy min kits (Qiagen), cells were enzymatically treated with 100uL  2U/uL lyticase solution (Sigma #L2524) for 10min at 30 degree C. RNA libraries were prepared according to standard Illumina protocols