GSM2973237: SCC25_ETS1_C20_Rep2; Homo sapiens; ChIP-Seq
Chromatin was extracted from Mesenchymal HNSCC Cell Line (SCC25) using a low SDS shearing kit (Diagenode: C01020010). Lysates were sonicated with a Bioruptor for 30-40 min. IPs were carried out using the Active-Motif High Sensitivity ChIP Kit using the C20 (Santa Cruz) and 501A (Bethyl Labs) antibodies for ETS1. Libraries were prepared using ThruPLEX DNA-seq kits from Rubicon Genomics. The samples were then 50 bp single-end sequenced on an Illumina HiSeq 2500.