GSM3895928: Young 2 Lung; Mus musculus; RNA-Seq
Single cell suspensions for each tissue were prepared after dissecting tissues with a razor blade into small pieces. Kidney and lung were digested in liberase DL while speen further mechanically dissociated with a plunger. Cells suspensions were filtered through 100 um, 70 um, and 40 um filters before treatment with ACK Lysing Buffer. Cells were washed 2 times in cold PBS, counted on a TC20 Cell Counter, and diluted to 1e6 cells / mL. 6,000 cells/sample were then loaded onto each of two lanes in a 10X Chromium v2 Single Cell Transcriptomics microfluidics chip and emulsified. Libraries were constructed following the 10X Genomics 3' Single Cell transcriptome v2 protocol.