GSM4151172: dbdb1; Mus musculus; RNA-Seq
Total RNA was extracted from the renal cortex of each mouse (n = 3 for each group) using TRIzol reagent (Invitrogen, Carlsbad, CA, USA).A total amount of 3 μg RNA per sample was used as input material for the RNA sample preparations. Ribosomal RNA was removed with an Epicentre Ribo-zero™ rRNA removal kit (Epicenter, USA) according to manufacturer's instructions. Sequencing libraries were generated using the rRNA-depleted RNA by NEBNext® Ultra™ Directional RNA Library prep kit from Illumina® (NEB, USA).