RNA-Seq of canis lupus familiaris:adult canine osteosarcoma of the tibia
TruSeq stranded ribosome-depleted libraries were constructed from 500ng total RNA from a canine tibial osteosarcoma lesion and single indexed.  External RNA Controls Consortium (ERCC) spike-in was added to the RNA libraries (Invitrogen, 4456740).  The RNA libraries were pooled and sequenced on 2 lanes of the HiSeq2500 1T platform as 2 x 125 bp reads.