16S rRNA secs from feces of a non-IgE mediated CMPA infant one week after oral challenge
Partial 16S rRNA gene sequences were amplified from the extracted DNA from feces of a non-IgE CMPA (cow's milk protein allergy) infant after a week of receiving a standardized oral challenge with cows milk formula.  PCR was performed using the primers pair Probio_Uni and /Probio_Rev, which target the variable region V3 of the bacterial 16S rRNA gene according to previous reports (Milani, Hevia et al. 2013) . Samples were submitted to 2 x 250 bp paired-end sequencing by means of an Illumina MiSeq System (Illumina, San Diego, USA) at the DNA sequencing facility of GenProbio SRL (Parma, Italy).