Illumina HiSeq 2000 paired end sequencing; RNA seq of B16 mouse melanoma cell line upon knockdown of gene myg1
RNA seq of B16 mouse melanoma cell line upon knockdown of gene myg1
myg1 siRNA transfected and control siRNA transfected B16 mouse melanoma cells were trypsinized and harvested RNA was extracted using Machery-Nagel triprep kit TruSeq RNA library prep kit V2 was used to prepare the RNA library