GSM2535504: 48h +T Rep 1; Mus musculus; RNA-Seq
RNA was isolated using Trizol (ThermoFisher Scientific). mRNA was enriched using beads, first and second strand cDNA synthesis (+Actinomycin D). Library preparation was done using Kapa Library Preparation Kit (Roche). Barcoded samples were multiplexed and sequenced 100bp PE on a HighSeq2000 (Illumina).