GSM3664899: Skov3_TET1; Homo sapiens; RNA-Seq
Total RNA was quantified using a Nanodrop ND-1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA), checked for purity and integrity on a Bioanalyzer-2100 device (Agilent Technologies, Santa Clara, CA), and performed the next-generation sequencing. Libraries were prepared using TruSeq RNA Sample Preparation Kits (Illumina, San Diego, CA, USA), according to the manufacturer's protocols. Specifically, each library was paired-end sequenced (2 × 150 bp) on a HiSeq-4000 platform (Illumina, San Diego, CA, USA). The RNA reads were then aligned to the human reference genome hg19, using Bowtie2 V2.2.6 (SourceForge, Slashdot Media, La Jolla, CA, USA), and relative gene expression was quantified as transcripts per million (TPM) and Fragments Per Kilobase of transcript per Million (FPKM) mapped reads, using RSEM v1.2.17 (GitHub, San Francisco, CA, USA). Libraries were prepared using TruSeq RNA Sample Preparation Kits (Illumina, San Diego, CA, USA), according to the manufacturer's protocols. Specifically, each library was paired-end sequenced (2 × 150 bp) on a HiSeq-4000 platform (Illumina, San Diego, CA, USA).