GSM1357040: Ctrl_rep2_RNASeq_TGACCA; Xenopus (Silurana) tropicalis; RNA-Seq
400ng of mRNA was used for library preparation following methods published in Tan et al. Genome Research 2013. Total RNA was isolated by homogenizing embryos in SDS/PK buffer for 1 hr, followed by phenol/chloroform extraction and ethanol/NH4OAc precipitation.  mRNA was isolated from total RNA using Oligo-DT Dynabeads (Invitrogen).