GSM3356381: RNAseq_ENB_21T; Homo sapiens; RNA-Seq
RNA extraction was performed using the RNeasy Kit (Qiagen) according to the manufacturer's instructions. Quality control of extracted nucleic acids was done using Agilent® bioanalyzer. Total RNA for each sample was converted into a library of template molecules for sequencing on the Illumina HiSeq 2000 according to the NuGen Ovation RNA-Seq System V2 protocol. Libraries were quantitated by qPCR (KAPA Systems), multiplexed and sequenced, 4 samples per lane, on the HiSeq2000 using 75 bp paired-end sequencing.