Didymella segeticola isolate: GZSQ-4 Genome sequencing
Genomic DNA was extracted from Didymella segeticola GZSQ-4, and then evaluated its quality and purity using Qubit 3.0. Genomic DNA was further treated as the fragment with the size of 8-10 k. Both ends of single strand were linked with positive and negative strand of double strands, and formed the structure of SMRT Bell. SMRT Bell templates were annealed and bonded with polymerase, then sequenced using PacBio RS.