GSM4101945: RNA-seq T11F80L_8 rep1; Homo sapiens; RNA-Seq
Total RNA was extracted using the Qiagen RNeasy kit (Qiagen 74104) according to the manufacturer protocol. The RNA was eluted in water and quantified. Samples quality control and sequencing was done by the UNIL Genomic Technology Facility (GTF). Ribosomal RNA was removed using the Ribo-zero rRNA removal kit (Illumina MRZH11124). Libraries were prepared using the truSEQ stranded RNA LT kit (Illumina) and 80-nucleotides single-read high-throughput sequencing was performed with 6 samples per line (multiplexing).