GSM3664717: sox10 + cells unij-Pro exp1; Danio rerio; RNA-Seq
Total RNA was extracted with TrizolPlus RNeasy mini kit from Qiagen. Total RNA was depleted of ribosomal RNA with the RiboGone Kit (Clonetech). Enriched mRNA was then applied to library preparation according to manufacturer's protocol (Smart Low input Protocol, Clonetech). After repeated quality control for average DNA input size of 300 bp, the samples were sequenced on a HiSeq Illumina sequencer with 2x100bp paired-end reads.