GSM4154277: RNA_Eomes_null_rep_2; Mus musculus; RNA-Seq
RNA was isolated from Flk-1hi/PdgfRa- single cell suspensions (1.5x105 cells) from three independent hematopoietic differentiations of WT (CCE) and Eomes-null (clone 6A6, CCE) cells using the RNeasy Micro Kit (QIAGEN, Cat#74004). These cell samples were taken from the same cells that were also used for ATAC-seq (see below). Total RNA was normalized to 800ng per sample, depleted of cytoplasmic and mitochondrial ribosomal RNA sequences (Ribo-Zero Gold rRNA Removal Kit (H/M/R), Cat: #MRZG12324) and library preparation was performed using the Illumina TruSeq Stranded Total RNA Library Prep (H/M/R) (Cat: #20020597), followed by sequencing (75-cycle paired end) on the Illumina HiSeq4000 platform.