GSM2535286: P14_Naive_1; Mus musculus; RNA-Seq
At least 500,000 P14 CD8+ T cells were sorted by FACS from spleens of mice. RNA was extracted using RNAqeous Micro RNA extraction kit. Pancreatic islets were extracted from the pancreata of Rip-LCMV mice at day 3 post-LCMV infection by collagenase digestion. Islets were hand picked and RNA was extracted using RNAqeous Micro RNA kit. Total RNA was depleted for ribosomal RNA using Ribo-Zero Gold Kit (MRZG12324). Library preparation was carried out using NEBNext UItra Directional RNA Library Prep Kit for Illumina (E7420L) and the libraries sequenced using Illumina NextSeq500.