GSM3407053: input [20180803_7416]; Mus musculus; ChIP-Seq
Purified immunoprecipitated DNA isolated using Qiagen PCR purification kit. ChIP Libraries were prepared with the Swift Biosciences Accel-NGS® 2S Plus DNA Library Kit according to kit instructions. Final libraries were quantified by qPCR with the KAPA Library Quantification kit from Roche and sequenced in single-read mode for 40 bases. Whole cell extracts were sonicated to solubilize the chromatin. The chromatin extracts containing DNA fragments with an average size of 200-400 bp were immunoprecipitated using different antibodies.