ONT Direct RNA-Seq of Dioscorea alata breeding line TDa95/00328
Total RNA was extracted from TDa95/00328 plants grown at ICRAF in Nairobi, Kenya from leaf petiole, roots, initial sprouting leaf, leaf bud, young leaf, semi-matured leaf, matured leaf, fifth leaf, bark, stem, first internode, and middle vine using the Purelink RNA mini kit. Libraries were prepared using the Oxford Nanopore SQK-RNA001 Direct RNA-Seq kit, loaded onto R9.4 SpotON Flow Cells, sequenced using a MinION, and bases-called using Guppy version 2.3.1.