GSM1846436: emer_amoA; soil metagenome; RNA-Seq
RNA was extracted from 0.5mg of dry weight soil using a POWER SOIL® (Epicentre) commercial kit according to the manufacturer’s instructions. The lysis phase was performed with zirconia beads. RNA libraries were prepared for sequencing using standard Illumina protocols. Libraries were prepared according to Illumina's instructions. Briefly, mRNA were separate of total RNA using (mRNA-prokaryotic mRNA isolation epicentre) kit. RNA transcripts were polyadenylated employing poly (A) polymerase Tailing kit epicenter and Later mRNA were amplified using T7 RNA polymerase and µMACSTM one step T7 template kit.