GSM1207827: RRBS_Postimplantation_embryo3_rep1; Homo sapiens; Bisulfite-Seq
Single cells were picked into the lysis buffer which contained protease to release the naked DNAs Cells were picked into the lysis buffer to release naked DNA; then the released DNAs were digested with MspI, then filled-in and tailed with an extra "A" , then ligated with Illumina indexed adaptors. Bisulfite conversion was performed under the manufacturer’s instructions of the MethyCode DNA Methylation Kit (Invitrogen) with minor modification, the converted DNAs were enriched by two rounds of PCR amplifications and size-selected after running 12% native polyacrylamide TBE gel. The quality-ensured libraries were used for pair-ended deep sequencing on Illumina HiSeq2000 Sequencer.