WGS of Coregonus suitderi : ONT sequencing
DNA was extracted using a phenol/chloroform protocol and gDNA quality and purity was assessed using spectrophotometry, fluorometry and capillary electrophoresis. Additional purification steps were performed using AMPure XP beads (Beckman Coulter). Library preparation and sequencing were performed using Oxford Nanopore Ligation Sequencing Kits SQK-LSK108 according to the manufacturers instructions. 10g of DNA was purified then sheared to 20kb using the megaruptor1 system (Diagenode). A DNA-damage repair step was performed on 5g of DNA. Then an END-repair-dA-tail step was performed for adapters ligation. Library was loaded onto 1 R9.4.1 flowcell and sequenced on a GridION instrument at a concentration of 0.1pM for 48 h.