Infected BALB/c macrophages replicate 1
Total RNA was purified from macrophages by using chloroform separation and isopropanol precipitation. Integrity and presence of contaminants in the RNA samples were assessed using the Agilent RNA 6000 Nano kit on a 2100 Bioanalyzer system. Libraries of complementary DNA (cDNA) fragments were generated from polyadenylated (poly(A)) RNA using the TruSeq RNA v2 sample preparation kit from Illumina.