WGS adult nose sample Pair578M-0
Metagenomic DNA samples were quantified by Quant-iT PicoGreen dsDNA Assay (Life Technologies) and normalized to a concentration of 50pg/uL. Illumina sequencing libraries were prepared from 100-250pg of DNA using the Nextera XT DNA Library Preparation kit (Illumina) according to the manufacturers recommended protocol, with reaction volumes scaled accordingly. Prior to sequencing, libraries were pooled by collecting equal volumes (200 nl) of each library from batches of 96 samples. Insert sizes and concentrations for each pooled library were determined using an Agilent Bioanalyzer DNA 1000 kit (Agilent Technologies).