GSM3581511: HepG2_Ribo_WT_ADRplus_rep2; Homo sapiens; OTHER
The HepG2 cells were treated with 500 ng/mL ADR for 24 h, and then cycloheximide (CHX) was added to inhibit the translational elongation of ribosomes. The 26-30-nt RPFs were isolated to generate ribosome profiling sequencing libraries. The ribosome profiling assay was performed as described in the Nature Method's protocol and the Ribo-seq libraries were prepared according to Illumina's instructions