Illumina HiSeq 2500 sequencing; RNA-seq of adipocyte progenitor cells derived from old and young mice to investigate the molecular signature of aging
RNA-seq of adipocyte progenitor cells derived from old and young mice to investigate the molecular signature of aging
Lin-/Sca-1+ APCs were collected according to the protocol in TJ Schulz et al (2011) (https://doi.org/10.1073/pnas.1010929108) RNA was extracted by using Qiagen kit by standard method.  RNA-sequencing libraries were constructed from total RNA using an Ovation RNA-sequencing system version 2 (NuGEN).