GSM2973438: mESC_Msl2KO_H3_ChIP; Mus musculus; ChIP-Seq
15 cm dishes were fixed in PBS containing 1% methanol-free formaldehyde for 8 min at RT. Chromatin was purified using Paro-washes as described in Baubec et al., Nature. Chromatin was fragmented using MNase as for the Drosophila ChIP protocol but digesting at 37C and adding SDS to 0.1% final concentration after addition of EDTA-containing buffer. Cleared chromatin was incubated for 6-8h with antibody, before addition of Protein A dynabeads for 1h. Beads were washed 3 x in RIPA, 1 x LiCl-DOC and 2 x in TE buffer. Chromatin was further processed according to standard procedures. NEB Next Ultra II