GSM1845110: control_sh_RZ1_RNAseq; Drosophila melanogaster; RNA-Seq
RNA-seq was performed according to Zhang et al, Silence, 2012 Total RNA was extracted using Trizol and depleted for ribosomal RNA (Ribo-Zero Kit, Epicentre). For 2nd strand synthesis, dUTP was added to generate stranded libraries. UDG digested dsDNA was cloned using the NEBNext™ DNA Sample Prep Reagent Set 1 (NEB). The Kapa HiFi Library Amp Real-Time Kit (Peqlab) with Illumina TruSeq compatible primers was used to amplify the library.