GSM4705300: CTL CFs - CFs from healthy donor rep3; Homo sapiens; RNA-Seq
CFs at 80% confluency were detached from culture plates and total RNA was extracted using a High Pure RNA Isolation Kit (Roche), according to the manufacturer's instructions. Sequencing library was prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, MA, USA) according to manufacturer's instructions. In brief, 200-300 ng of total RNA was used as input to the NEBNext poly(A) mRNA Magnetic Isolation Module and the enriched samples were then fragmented and transcribed into cDNA. Following universal adapter ligation, samples were barcoded using dual indexing primers.