GSM2536175: GFP_3; Danio rerio; OTHER
gDNA was collected from TB stage zebrafish embryos following instructions for the Qiagen DNeasy kit. Adenine-methylated gDNA was digested with DpnI, followed by ligation of DamID adaptors. Unmethylated DNA was destroyed by DpnII digestion, and methylated regions were PCR amplified in 2 parallel reactions per sample. Adaptors were removed by additional DpnII digest followed by addition of sequencing idexes.