GSM4704943: 25_S_japonica_1; Solenopsis japonica; RNA-Seq
RNAs samples were extracted from whole body of S. japonica adults using Trizol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instruction. After RNA extraction, it was resuspended in nuclease-free water and quantified using a spectrophotometer (NanoDrop, Thermo Scientific, Wilmington, DE, USA). cDNA was then synthesized from RNA (1 μg) using RT PreMix (Intron Biotechnology, Seoul, Korea) containing oligo dT primer according to the manufacturer's instruction. After RNA extraction, it was resuspended in nuclease-free water and quantified using a spectrophotometer (NanoDrop, Thermo Scientific, Wilmington, DE, USA). cDNA was then synthesized from RNA (1 μg) using RT PreMix (Intron Biotechnology, Seoul, Korea) containing oligo dT primer according to the manufacturer's instruction. RNA libraries were prepared for sequencing using standard Illumina protocols