GSM2532869: P28-DH-2; Rattus norvegicus; RNA-Seq
Total RNA was isolated from the infected cells using the RNeasy RNA extraction Mini-Kit (Qiagen) and the quality of the RNA was confirmed by agarose gel electrophoresis. The sequencing library was prepared using the Illumina TruSeqStranded mRNA Sample Preparation Kits (Illumina). The mRNA derived from total RNA using poly-T oligo-attached magnetic beads was fragmented and converted into cDNA. Adapters were ligated to the cDNA and the fragments were amplified by PCR.