Mycobacterium tuberculosis H37Rv, wild type
Mycobacterium tuberculosis strains were grown at 37C in 7H9 medium (Difco) supplemented with 0.2% glycerol, 0.05% Tween 80 and 10% albumin-dextrose-catalase (ADC, Middlebrook) or on 7H10 plates supplemented with 0.5% glycerol and 10% oleic acid-albumin-dextrose-catalase (OADC, Middlebrook). Sautons liquid medium was used for culture filtrate analysis. Streptomycin (20 g/ml), kanamycin (20 g/ml), hygromycin (50 g/ml) or 2.5% sucrose were added when necessary. Libraries were prepared using the Kapa LTP Library Prep kit (Kapa Biosystems) according to the manufacturers recommendations. Cluster generation was performed using the Illumina TruSeq SR Cluster Kit v4 reagents and sequenced on the Illumina HiSeq 2500 using TruSeq SBS Kit v4 reagents. Sequencing data were demultiplexed using the bcl2fastq Conversion Software.