PCR amplicon of 16S RNA gene (V1-V2 region) sequence of murine gut microbiota
PCR amplification of the gut microbial 16S rRNA gene of V1-V2 region was done using forward primer (5'-CCATCTCATCCCTGCGTGTCTCCGACTCAGNNNNNNNNNNagrgtttg atymtggctcag-3') containing  forward primer 27Fmod (agrgtttg atymtggctcag) and the 454 primer A, a unique 10-bp barcode sequence for each sample (indicated in N), and reverse primer (5'-CCTATCCCCTGTGTGCCTTGGCAGTCTCAGtgctgcctcccgtaggagt-3') containing the 454 primer B and reverse primer 338R (tgctgcctcccgtaggagt).