Hiseq-BSP of Sus scrofa: new born piglet jejunum tissues
For HiSeq-BSP sequencing, PCR products of multiple genes from one sample were quantified and pooled together equally. 1 μg of pooled PCR products was fragmented to a mean size of approximately 200 bp followed by end-polishing, A-tailing, adapter ligation and 5-cycle PCR to generate a barcoded library.