Whole genome sequencing of Nymphaea thermarum
For the mate-pair library we used the Illumina Nextera Mate Pair Library Preparation Kit with 1 g of input genomic DNA and followed the manufacturers protocol. Size selection was done using a Sage Science Pippin Prep machine with a target insert size of 3 kb, however, an Agilent Tapestation assay of the post-size selection product showed a peak at ~ 5 kb. The library was sequenced on an Illumina HiSeq 2500 sequencer with v4 chemistry. We collected 2x125 bp reads for the mate-pair library.