GSM5057859: Caco-2_Control_mRNA_rep3; Homo sapiens; RNA-Seq
Cells were lysed with the Qiazol Lysis Reagent (Qiagen, Hilden, Germany) for subsequent extraction of RNA using the Qiagen miRNeasy Mini Kit (Qiagen, Hilden, Germany). Nanodrop (Thermo Fisher Scientific, Waltham, MA, USA) was used to assess quality (260/280) and quantity. Total RNA samples were also QC checked using the Agilent High Sensitivity DNA Kit (Agilent Technologies, Santa Clara, CA, USA). Libraries for mRNA sequencing were prepared from total RNA samples using Illumina Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA). Each sample was sequenced on the Illumina NextSeq 550 to generate single-end 75 nucleotide reads. Libraries for miRNA sequencing were prepared from total RNA samples using NEBNext Multiplex Small RNA Library Prep Kit for Illumina. Each sample was sequenced on the Illumina NextSeq 550 to generate single-end 50 nucleotide reads.