Bru-seq Clone 2 Promoterless FHIT GM11713
Bru-seq nascent RNA sequencing (PMID 23973811). Cells were pulse-labeled for 30 min with bromouridine. RNA was harvested and subjected to immunoprecipitation with anti-BrdU antibodies prior to conversion to cDNA by random priming. A strand-specific library was constructed and subjected to high throughput sequencing.