WGS of Micromonospora sp. MW-13: TruSeq PCR-free library
For the isolation of gDNA, the culture was grown in 15 ml TSB (Tryptic Soy Broth) overnight at 30C on a rotary shaker (180 rpm). For gDNA isolation, the QIAGEN Genomic DNA Purification kit in combination with 100/G Genomic-tips (QIAGEN) were used according to the manufacturers protocol, except that for the bacterial lysis the handled volumes were doubled and incubation time at 50C was prolonged until a clear lysate was obtained.A paired-end library constructed (TruSeq PCR-free Library Kit) and subjected to sequencing using a Illumina HiSeq 1500 platform.