GSM2800532: iN_Bisulfite-seq; Mus musculus; Bisulfite-Seq
Genomic DNA isolated from control and ND1-transduced microglia was subjected to bisulfite treatment with the Imprint DNA Modification Kit (Sigma) according to the manufacturer’s instructions. We adopted a strategy termed post-bisulfite adaptor tagging (PBAT), which provides an unbiased methylome analysis (Miura et al., 2012). The multiplex PBAT method is described in detail on the CREST/IHEC Japan webpage (http://crest-ihec.jp/english/epigenome/index.html).