RNASeq of Vitis Rda1: Phomopsis-inoculated shoots
A subset of 12 resistant (scores 0 or 1) and 12 susceptible (score 2 or 3) progeny from ‘Chardonnay’ x V. cinerea B9 were selected to maximize the number of progeny with recombination events around the Rda1 resistance locus. On August 29th, 2013 three shoots on each field grown vine were spray-inoculated using a Preval hand-held paint sprayer (Preval, Illinois, USA) immediately before sunset and enclosed in a moistened plastic bag to maintain surface wetness. The next morning, inoculated stem internode between the second and third unfurled leaf was collected, immediately stored in liquid nitrogen, and transferred to the laboratory for RNA extraction. Barcoded, strand-specific, mRNA multiplexed libraries were prepared as previously described (Zhong et al. 2011).