Fungal diversity analysis: eggs sample
Gemonic DNAs were extracted from the egg samples. Primers ITS3 and ITS4 were used to amplify the ITS2 region of the fungal gemonic DNAs. A bar code sequence was added at the 5-end to discriminate each sample. PCR was conducted in triplicate, and the products were pooled for each subsample. A composite DNA sample for sequencing was created by combining equimolar ratios of amplification products from each sample.