IlluminaSeq of LdNPV: Virus population in field collected gypsy moths
We amplified samples using the whole genome amplification REPLI-g UltraFast Mini kit from Qiagen, following the standard Qiagen protocol. After amplification, DNA concentrations were re-quantified in a spectrophotometer and then normalized to 50 ng/ul. We used the Nextera DNA Sample Prep Kit (Illumina-compatible GA0911-96), following the standard protocol for use with custom adaptors, to prepare libraries for Illumina sequencing with custom barcodes.