GSM3665367: 56d Control_c; Trematomus bernacchii; RNA-Seq
Total RNA from n=4 fish per treatment per time point was extracted in Trizol using an automated TissueLyser II (Qiagen) according to manufacturer's recommended protocol. The quality of RNA extracts was determined by gel electrophoresis and peak analysis on an Agilent 2100 bioanalyzer. Only RNA samples with RIN scores greater than 8 were utilized for library preparation and subsequent sequencing. The Illumina Truseq Small RNA library prep kit and size selection with the BluePippin automated gel extraction system was carried out to create small cDNA libraries. 50 bp SE sequencing libraries were then indexed and pooled prior to sequencing on 2 lanes of an Ilumina 4000 HiSeq platform housed in the UC Davis Genome Center.