Rhynchophorus ferrugineus  gut microbiota by 16S rRNA gene-based Illumina high-throughput sequencing
The variable region V3 + V4 region of 16s rDNA was amplified by primer with barcode. The primer sequence used was: 341F: CCTAYGGGRBGCASCAG; 806R: GGACTACNNGGGTATCTAAT. The PCR product was then sequestered and quantified by Qubit3.0 fluorometer (Thermo Fisher Scientific, USA). The purified amplification products were mixed in equal amounts, ligated and sequencing library was constructed