GSM4932154: Poly_CHX_Inp_WT_Rep_1; Homo sapiens; RNA-Seq
RNA was precipitated from each fraction using 2 volumes of 100% ethanol followed by extraction using TRIzol Reagent. Fractions were spiked in with a luciferase RNA probe and combined to generate light and heavy polysomes pools. Sequencing libraries for monosome, light polysome, and heavy polysome samples were constructed with the Illumina TruSeq Stranded Total RNA Library Prep Kit (RS-122-2201), including RiboZero treatment. Libraries were multiplexed on two lanes of an Illumina HiSeq3000 instrument in paired-end mode.