UMB1465 10x Genomics single cell RNA sequencing (scRNAseq) of prefrontal cortex oligodendrocytes sorted with anti-SOX10: Sample UMB1465_3065-pfc-OligoSOX10_3065-scRNAseq
"scRNA-seq was performed using the 10X Genomics Chromium Next GEM Single Cell 3' Reagent Kits v3.1. Fresh frozen human brain tissue was processed to obtain nuclear pellets. 10,000 to 15,000 single nuclei were sorted using cell-type specific antibodies for each experiment in a tube containing RT master mix, and immediately processed for GEM generation, barcoding, and cDNA amplification, following manufacturer instructions. Each library preparation was submitted for paired-end single indexing sequencing on one lane of Illumina HiSeqX or NextSeq500 targeting ~50,000 read pairs per nucleus."