RNAseq of enriched active Arabidopsis thaliana telomerase from seedlings: telomerase null mutant, tert, stranded library
Telomerase protein complexes from five-day-oldtertnull mutant seedlings wereprecipitated with PEG8000. The pellets wereresuspended andsize fractioned through aSuperose6 Increase 10/300 GL column. The same fractions collected from wild-type plants were collected for the telomerase mutants andimmunoprecipitated with anti-AtTERT antibody. Libraries wereconstructed by theNEBNext Ultra Directional RNA Library Prep Kit and sequenced on an 300x2 Illumina MiSeq platform