GSM4391883: anti-LTbR_20_Hours [RVS_008]; Homo sapiens; RNA-Seq
Following stimulation for the indicated times, monolayers were washed with warm PBS, detached using TrypLE express. Cell pellets were split and processed for western blot analysis or lysed with QIAGEN RLT buffer. RNA was isolated using QIAGEN RNA easy extraction protocol RNA libraries were prepared for sequencing using standard Illumina protocols