GSM2197451: white-embKD; Drosophila melanogaster; RNA-Seq
Total RNAs were isolated from ovaries using TRIzol. 4µg of total RNA were subjected to Ribo-Zero ribosomal depletion (Epicenter). RNA was further purified using RNA Clean & Concentrator-5 (Zymo Research). Library construction and 150nt paired-end read sequencing were performed by Donnelly Sequencing Centre (Toronto) on an Illumina NextSeq 500. The RNA-seq experiments were done in two biological replicates.