Ovary queenright workers - Colony C
Total DNA was extracted with DNeasy Blood and Tissue KitTM (Qiagen) and treated with EZ-DNA methylation kit, following standard recommendations. Then samples were PCR amplified using especific primers and amplicons were sequenced twice in two distinct lanes. Files names are designed as follow: tissue source (e.g., Brain or Ovary, "Br" or "Ov", respectively), source colony (e.g., A-F), social context (e.g., Queenright or Queenless, "m" or "p", respectively), lanes (e.g., 1 or 2) and run (e.g., 1 or 2).