GSM1845109: aub_sh_ago3_sh_RNAseq; Drosophila melanogaster; RNA-Seq
RNA-seq was performed according to Zhang et al, Silence, 2012 Total RNA was extracted using Trizol and polA RNA was purified with oligo-dT beads. For 2nd strand synthesis, dUTP was added to generate stranded libraries using the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB). The Kapa HiFi Library Amp Real-Time Kit (Peqlab) with Illumina TruSeq compatible primers was used to amplify the library.