GSM1207205: 2nd_neuron_rna_seq; Homo sapiens; RNA-Seq
Total RNA was isolated from cells using the miRNeasy Kit (Qiagen) according to the manufacturer’s protocol.  An additional DNAse1 digestion step was performed to ensure that the samples were not contaminated with genomic DNA. RNA purity was assessed using the Agilant 2100 Bioanalyzer (Beijing Genomics Institute). Each RNA sample had an A260:A280 ratio above 1.8, a RIN>9, and a A260:A230 ratio above 2.2. Briefly, total RNA was converted to cDNA, which was then used for Illumina sequencing library preparation. RNA libraries were prepared for sequencing using standard Illumina protocols