GSM5076237: mouse_OM_scSeq; Mus musculus; RNA-Seq
Olfactory mucosa was dissected from respiratory tissue and underlying bone. Enzymatic digestion with collagenase, hyaluronidase, dispase, and trypsin released the cells. Neurons and sustentacular cells were selected against using surface marker staining and FACS. Library construction proceeded immediately. Constructed using 10X Genomics Single Cell Gene Expression kit following manufacturer's instructions.