GSM1205154: FLM.RNA; Tuber melanosporum; RNA-Seq
Total RNA from FLM and FB was extracted as described previously (Montanini et al. 2003) and purified with the RNeasy Plant Mini kit and on-column DNase I digestion (QIAGEN) following the manufacturer’s instructions. RNA integrity was verified by a Bioanalyzer: RIN scores of 7.0 and 6.5 for FB and FLM, respectively. RNA was quantified with the Qubit RNA BR Assay and 1 μg of purified RNA was utilized as starting material for library construction, which was carried out with the Illumina TruSeq RNA Sample Preparation kit following the manufacturer's instructions. Libraries were sequenced using 50-bp single-end reads on the HiSeq 2000 System (Illumina).