GSM5221159: GLOE HCT116 WT UT R3; Homo sapiens; OTHER
GLOE-seq was performed according to the published protocol (Sriramachandran et al., 2020; doi:10.1016/j.molcel.2020.03.027) with minor modifications as described below. Briefly 5x10^6  of each HTC116 cells under different treatment conditions were harvested and individually embedded in an agarose plug for genome DNA extraction. After splinter adaptor ligation, sonication and clean-up of genomic DNA, each HCT116 sample was adjusted and normalized to 15.5ug input DNA amount. Genomic DNA from mouse embryonic stem cells (E14, 129/Ola) prepared in the same manner was added as a spike-in at 5% (w/w) final concentration before affinity capture by streptavidin magnetic beads. GLOE-seq libraries were prepared as described (Sriramachandran et al., 2020' doi: 10.1016/j.molcel.2020.03.027) and double size-selected with AMPure XP SPRI beads at 0.4X-0.8X before sequencing on the Illumina NextSeq 500 platform.