GSM1845131: control_sh_germ_GFP_Piwi_GFP_IPseq_ox_R2; Drosophila melanogaster; RIP-Seq
small RNAs from beads were isolated by acid phenol extraction and Ethanol precipitation, further cloning of small RNAs was done as for total small RNA-seq small RNAs were PAGE selected and 3' and 5' linkers was ligated. 1st strand cDNA synthesis was performed using SuperScript III (Invitrogen) and the library was amplified using the Kapa HiFi Library Amp real time Kit (Peqlab) with Illumina TruSeq compatible primers. IP from ovary lysate/acid Phenol RNA extraction from beads/ sizeselected small RNA cloning