GSM3790071: Strand-specific RNA-seq in siCtrl 3 treated cells- Replicate #2 [U2OS]; Homo sapiens; RNA-Seq
Total RNAs were prepared using the MasterPure RNA Purification Kit (Epicentre Biotechnologies) supplemented with Baseline-ZERO DNase (Epicentre). EMBL-GeneCore performed the ribosomal RNA depletions. Library construction (NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®) was performed by EMBL GeneCore (Heidelberg, Germany) (stranded rRNA-minus RNA-seq). The 10 samples were barcoded, sequenced paired-end in one lane by NextSeq 500 Illumina device and demultiplexed.