GSM4766854: LSD1_MidK_chrX; Mus musculus; ChIP-Seq
Lysates were clarified from sonicated chromatins and LSD1-DNA complexes were isolated with antibody. Libraries were prepared using KAPA HyperPlus Kit (Roche) according to the manufacturer's instructions. Briefly, 10 ng of ChIP DNA was used for library preparation with unique dual index adaptor from IDT for Illumina TruSeq DNA UD Indexes (Illumina). The libraries were quantified using Qubit 4 Fluorometer (Thermo Fisher Scientific) and analyzed for size distribution using MultiNA (Shimdzu) or TapeStation 2200 (Agilent Technologies).