GSM1568711: hDIS3_RNB_mut_RNA-seq; Homo sapiens; RNA-Seq
RNA was isolated using TRI-Reagent (Sigma-Aldrich) Total RNA was treated with Turbo-DNase (Life Technologies), and then rRNA was removed from the samples using Ribo-Zero Kit (Epicentre), according to the manufacturer's protocols. As a control of experiment performance, external RNA (ERCC RNA Spike-In Mix; Life Technologies) was added to the samples. RNA libraries were prepared using TruSeq RNA Sample Preparation Kit (Illumina)