Transcriptome Assembly
The cDNA library was built using 2 ug of total RNA, in an Ion Chef System (Thermo Fisher Scientific) and the Ion Total RNA-Seq Kit v2 for whole transcriptome libraries (Life Technologies Corporation, California, USA), according to manufacturer instructions. The next generation sequencing was carried out using the semiconductor technology through an Ion S5 System (Thermo Fisher Scientific) using an Ion 540 sequencing chip.