GSM3896226: m1A-quant-seq, "-AlkB", replicate2; Homo sapiens; RNA-Seq
RNA samples were extracted from HEK-293T cells using the TRIzol reagent and standard protocol; mRNA were enriched by the Dyna-Oligo(dT)25 beads and m1A IP was performed with the m1A antibody. Libraries were constructed using ligation-based library construction approach incoporating the newly developed RT in the current work.