GSM3723136: HiC [10_Naive_BatfKO]; Mus musculus; Hi-C
activated CD4 T cells were harvested, washed with 1X PBS, cross-linked with 1% formaldehyde and nuclei were isolated. Cross-linked DNA was digested with HindIII and marked with biotin. Biotinylated DNA was captured using magnetic streptavidin beads (Invitrogen), used for adaptor ligation reaction, and PCR amplification using NEBNext DNA Library Prep Master Mix Set for Illumina and NEBNext Multiplex Oligos for Illumina (Index Primers Set, NEB). Hi-C libraries were sequenced with paired-end 50 bp using Illumina HiSeq 2500