GSM3581509: HepG2_RNA_WT_ADRplus_rep2; Homo sapiens; RNA-Seq
For RNA-seq profiling, the total RNA was purified from HepG2 and HepG2-KO-p53 cells untreated or treated with 500 ng/mL ADR for 24 h, with two independent samples, respectively. The total RNA was isolated from cells using the RNA isolation reagent Trizol (Invitrogen). Paired-end and strand-specific RNA-seq libraries were prepared according to Illumina's instructions and sequenced on Xten (ANOROAD) with a sequence length of 150 bp.