GSM1356860: 5_21_C; Gasterosteus aculeatus; RNA-Seq
pools of whole embryos from each mother were flash frozen on dry ice, homogenized, and total RNA isolated using AllPrep DNA/RNA Micro Kit (Qiagen). After quality control 1ug of total RNA was used for construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols (Illumina TruSeq Stranded RNAseq Sample Prep kit )