RNA-Seq of canis lupus familiaris:match adult canine pulmonary metastatic lesion
TruSeq stranded ribosome-depleted libraries were constructed from 500ng total RNA from a canine radial osteosarcoma lesion and single indexed.  External RNA Controls Consortium (ERCC) spike-in was added to the RNA libraries (Invitrogen, 4456740).  Exome capture utilized the Nimblegen Exome-Plus reagent (Nimblegen, 4000022470), covering ~152 Mb, as previously described.  The RNA libraries were pooled and sequenced on 2 lanes of the HiSeq2500 1T platform as 2 x 125 bp reads.