GSM1204480: AcH3_Butyrate (S01037); Mus musculus; ChIP-Seq
Sheared chromatin DNA was extracted using Magnify ChIP kit and Covaris S220 accroding to manufacture's procedure followed by precipitation of chromatin-DNA complex Libraries were prepared according to NEB's instructions accompanying the NEBNext® Ultra DNA Library Prep kit for Illumina.  Adapter ligated DNA was PCR amplified with Illumina primers for 18 cycles. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Hi-Seq 1000 following the manufacturer's protocols.