GSM3408817: ABA_4h_poly(A)+; Arabidopsis thaliana; RNA-Seq
Total RNA was extracted with TRIzol (Invitrogen) and treated with DNase I (Ambion). Polyadenylated [poly(A)+] RNAs were isolated from total RNA through two rounds of purification with oligo(dT) beads (QIAGEN). Libraries for RNA-seq were generated using NEXTflex Directional RNA-Seq Kit (dUTP based)  according to the manufacturer's instructions.