A genomic and transcriptomic approach to investigate the blue pigment phenotype in  Pseudomonas fluorescens
Library construction was performed using Encore® Complete Prokaryotic RNA-Seq (NuGEN) and fragmentation was performed using Covaris S-Series (duty cicle=10%; intensity=5; time=180s, intensity/burst=200) . The quality of libraries was verified using 2100 Bioanalyzer (RNA 6000 Nano total RNA Kit, Agilent Technologies). Sequencing was performed on HiSeq 2500 50 pair-end (UC Davis Genome Center).