V4 16S rRNA Community Sequencing of Homo sapiens: Laryngotracheal Swabs
PCR amplification of the V4 of the 16S rRNA region using KAPA HiFi 2x polymerase. PCR products were run on 1% low-melt agarose gels and bands at ~400-450 bp were cut out and and equimolar pool was created of all the samples before being sequenced on the MiSeq.