GSM3722634: WT_Ole_TG_390; Mus musculus; RNA-Seq
Cell lysates were thawed and RNA was purified using RNeasy Plus Micro Kit (Qiagen) according to manufacture guidelines (Protocol: Purification of Total RNA from Animal and Human Tissues). Purified RNA was stored at -80C. For library preparation RNA was used for the bulk MARS-seq  protocol (Jaitin et al. 2014 Science.343:776-9). MARS-seq libraries were sequenced using Illumina NextSeq 500 High Output v2 Kit (75 cycles). Bulk Mars-Seq (3' end-RNA-seq)