Stomach metagenome of Astyanax mexicanus: Surface Rascon adult male
The complete stomach microbiome for a A. mexicanus sample from Rascon surface population was assayed using the Ion 16S Metagenomic kit (LifeTech A2616). Amplification occurred in two multiplex pools (one for the V2, V4, and V8, and one for the V3, V6-7 and V9), with 25 PCR cycles, using the Ion Xpress Barcoded adapters. Libraries were quantified using Real-Time PCR, normalized and pooled. Emulsion PCR used Ion PGM Template OT2 400 kit.