Populus tomentosa tension wood xylem transcriptome- NW
Total RNA  were isolated by a modified CTAB method with isopropanol instead of lithium chloride for RNA precipitation. Extracted RNA was used for RNA library construction. Qualified total RNA was purified by RNeasy micro kit (Cat#74004, QIAGEN). Total RNA after purified were processed by TruSeq RNA Sample Preparation Guide to build cDNA library. The sequencing of cDNA was carried by Shanghai Biotechnology Corporation (Shanghai, China) using Illumina HiSeq™ 2000 follow cBot User Guide and HiSeq 2000 User Guide with paired-end program and data were control by data collection software.