GSM1847018: MCF7_RNA-Seq_R1; Homo sapiens; RNA-Seq
Total RNA was isolated from Trizol using Direct-zol RNA mini-prep kit (Zymo Research #R2050) with DNaseI digestion. RNA libraries were prepared for sequencing with the TruSeq Stranded Total RNA with Ribo-Zero Gold kit using standard Illumina protocols with the exception that library amplification was performed using KAPA HiFi™ Real-Time PCR Library Amplification Kit (Kapa Biosystems #KK2701). Illumina Barcodes used: MCF10A_sample1_AR002; MCF10A_sample2_AR004; MCF10A_sample3_AR001; MCF7_Sample1_AR002; MCF7_Sample2_AR004; MCF7_Sample2_AR005