RNA-seq of Candida albicans: Carbon
5 g of extracted RNA was treated with 2 units of TurboDNAse (Invitrogen) in a 50 l reaction. To inactivate the DNAse, NaCl, Tris-HCl pH7.5, and EDTA, were added to a final concentration of 500 mM, 200 mM, and 10 mM respectively. The RNA was PCI extracted and the supernatant containing the RNA was purified over a Zymo Research RNA clean up column and eluted with 15 l of nuclease free water.