RNA-Seq of Adansonia digitata leaf
Illumina TruSeq RNA libraries were prepared at the University of Wisconsin- Madison Biotechnology Center (Madison, WI) as follows. DNAs were purified using Agencourt AMPure XP beads (Beckman Coulter, USA). Quality and quantity of the finished libraries were assessed using an Agilent DNA1000 chip (Agilent Technologies, Inc., Santa Clara, California, USA) and Qubit dsDNA HS Assay Kit (Invitrogen, Thermo Fisher Scientific, USA), respectively. Libraries were standardized to 2nM. Cluster generation was performed using standard Cluster Kits and the Illumina Cluster Station (Illumina Inc., San Diego, CA, USA).