GSM3355389: HEPS_B+Dox_Rep2; Homo sapiens; RNA-Seq
HEPs (CD31+CD34+CD45-) and CD45+ cells were FACS-purified from three independent biological replicates. Pools of 50 cells were sorted directly into 2.3 μl of lysis buffer (0.2 % (v/v) Triton X-100 and 2 U/μl RNase inhibitor (Clontech)) in 96 well plates and stored at −80 °C. RNA-seq was carried out using the Smart-seq2 protocol according to Picelli S et al. (2014). Single-cell RNA-seq analysis was performed as described previously (Picelli et al., 2014). RNA libraries were prepared for sequencing using the Illumina Nextera XT DNA preparation kit. Pooled libraries were run on the Illumina Hi-Seq 2500.