GSM2198134: NCDS001162; Canis lupus familiaris; RNA-Seq
Total RNA was isolated by Asuragen from tissue homogenate with KingFisher Pure RNA Tissue Kit (Thermo Scientific). Isolation of miRNA from 50 μM tissue samples used a lysis buffer (RLT [Qiagen] + 1% β  mercaptoethanol) and a rotor-stator homogenizer A TruSeq Small RNA Library Kit (Illumina) was used for library construction of dog miRNA