GSM4932137: NaBH4_NAT10_Rep_1; Homo sapiens; OTHER
Total RNA was purified from wildtype and NAT10-/- HeLa cells using TRIzol Reagent followed by treatment with TurboTM DNase I Illumina libraries were constructed from two biological replicates using the NEBNext® UltraII™ Directional RNA Library Prep Kit for Illumina® (NEB, Cat. #: E7760) following manufacturer's suggestions with the reverse transcription step performed at 48 °C. Libraries were multiplexed on an Illumina HiSeq2500 instrument using TruSeq V4.0 chemistry and sequenced for 126 cycles in paired-end mode