GSM3790489: MOLM13 DMSO R273H_rep2; Homo sapiens; RNA-Seq
0.5x10e6 cells were collected, washed with ice-cold PBS followed by total RNA extraction using RNeasy kit (Qiagen, #74106). The quality of extracted RNA was determined using RNA ScreenTape (Agilent). RINe values were always >8.0. RNA was quantified by Qubit (ThermoFisher). 500 ng of RNA was used to prepare Illumina-compatible 3'-end sequencing libraries using QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen) and then sequenced using a NextSeq™ 550 Sequencing System (Illumina) to obtain single-end reads.