Illumina HiSeq 2500 sequencing; IMPC high throughput RNA seq of 1135 lines
IMPC high throughput RNA seq of 1135 lines
Trizol extraction as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina-compatible (GAIIx and HiSeq2000 platforms) RNA-seq libraries as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426