Mnase-seq of WT S. cerevisiae: Nascent chromatin from 5min EdU pulse
cells were arrested with alpha-factor in G1 and released for 45 minutes into S-phase. Cells were then labeled for 5 minutes with EdU followed by a 0 min thymidine chase (nacent chromatin). Whole chromatin was extracted and digested with MNase. Paired-end sequencing libraries were prepared without size selection.