A comprehensive analysis of microRNA in FSHD and control myogenesis
Deep sequencing of 3 control and 3 FSHD primary myoblast cell lines (purchased by Myobank-AFM Institut de Myologie, Paris, France), before and after in vitro myogenic differentiation, was performed using Illumina HiSeq 2000 platform. As a result, a total of 15X107 high quality clean reads were obtained and mature miRNAs make up the majority of sequences in 18-25 nts size range, with a clear peak at 22 nt in all samples. DEseq analysis has been performed to identify differentially expressed microRNAs in control and FSHD differentiation (myotubes vs. myoblasts), as well as in myoblasts/myotubes FSHD vs. control.