Sequencing of fragments enriched by selection of fecal functional metagenomic library in mouse: 13, Monocolonized microbiome, Human Diet, Prebiotic: None, Day: 1
Genomic dna from intestinal contents or fecal material was PCR-amplified using primers flanking the multicloning site of the pZE21 vector, sheared, and ligated to sequencing adaptors as in PMID 22936781.