GSM3665527: Input_Prol_rep3_RIP-seq; Homo sapiens; RIP-Seq
Cells were collected and nuclei were isolated and icubated with specific antibody. Total input RNA or RNA bound to SMARCB1 was retrieved and DNAse-treated. rRNA was depleted using Ribo-Zero Kit and polyA+ and - RNA was used for library preparation performed with Illumina's TruSeq Stranded Library Prep Kit. Library prep and sequencing was performed at EMBL Genomics Core Facilities