16S rRNA Amplicons of Rana pipiens: skin microbiome of individual exposed to antibiotics in the mesocosm
We isolated DNA from skin swabs using the PowerSoil DNA Isolation Kit (MoBio Labora- tories Inc., Carlsbad, CA) following the protocol described in Herna ndez-Go  mez et al. (2017a). In brief, we implemented two sequential PCRs to prepare our 16S rRNA V2 region amplicon sequencing library using the primer pair 27F/338R (Fierer et al., 2008) and following the methodology described in Herna ndez- Go  mez et al. (2017b). We shipped the barcoded sample pool on dry ice overnight to the Cornell University Biotechnology Resource Center (Ithaca, NY). We sequenced the sample pool on a MiSeq machine (Illumina, Inc. San Diego, CA) using the reagent kit V2 to produce 250 base pair (bp) paired-end reads.