GSM2970151: 4T1  bulk rep3; Mus musculus; RNA-Seq
Total RNA was isolated from 2 x 10^6 cells ausing the TriZol reagent (Thermo-Fisher Scientific) and following manufacturer's instructions. Libraries for RNA sequencing were generated using TruSeq RNA sample preparation kit v2 (Illumina Inc., San Diego, CA, USA) following manufacturer's instructions, using 1 µg of total RNA as input material and 15 PCR cycles for DNA amplification. Libraries were quantified by Qubit 2.0 Fluorometer using ds DNA High Sensitivity Qubit Assay (Thermo-Fisher Scientific) and pooled together in equimolar amounts.