Identification and characterization of a Rhodobacter capsulatus strain A12 with disease suppression and bioremediation function
Genomic DNA was extracted with the SDS method. A total amount of 1g DNA per sample was used as input material for the DNAsample preparations. Sequencing libraries were generated using NEBNext UltraDNA Library Prep Kit for Illumina (NEB, USA) following manufacturersrecommendations and index codes were added to attribute sequences to each sample.Briefly, the DNA sample was fragmented by sonication to a size of 350bp, then DNAfragments were end-polished, A-tailed, and ligated with the full-length adaptor forIllumina sequencing with further PCR amplification. At last, PCR products were purified(AMPure XP system) and libraries were analysed for size distribution by Agilent2100Bioanalyzer and quantified using real-time PCR. The whole genome was sequenced using Illumina NovaSeq PE150 platform.