GSM2627477: N654het_dim1-wg-seq; Neurospora crassa; OTHER
Cultures were grown in Vogels medium and 1.5% sucrose (standard minimal medium), shaking for 48 hours at 32oC. Genomic DNA was isolated using a protocol modified from (Oakley et al, 1987). Whole genome sequencing was done with the Nextera kit (Illumina) following manufacturers’ protocol, except that after the PCR amplification step, libraries were purified using a Qiagen MinElute kit (PCR purification protocol), fragments between 350 – 550bp were size selected from a 2% TAE gel following gel electrophoresis, and final libraries were purified using a Qiagen MinElute kit (Gel Extraction protocol).