Amplicon sequencing of MRE3'control: blood fed mosquito 27
RNA was extracted from blood-fed mosquitoes infected with MRE3control. The RNA was reverse transcribed to DNA using a Sindbis virus specific primer. PCR was used to amplify the 3UTR region and add adaptor sequences. 60ng of PCR product was used for index PCR. Libraries were validated, quantified and pooled, then sequenced with 559 2x150bp Illumina MiSeq run using MiSeq 300 cycles reagent micro kit v.2.