GSM1844489: WT rep3; Danio rerio; RNA-Seq
Yap S87A and sibling control whole eyes were dissected at 36 hpf and immediately frozen on dry ice until ~60 pooled retinas were obtained for each genotype. RNA was purified as described in (Uribe et al., 2012) except that RNA was eluted in a 50μl final volume. RNA quality was determined using an Agilent BioAnalyzer.  50-bp single read sequencing was performed in triplicate for each genotype using an Illumina HiSeq2000 at VANTAGE (Vanderbilt University, Nashville, TN). Sequencing results were analyzed by VANGARD (Vanderbilt University, Nashville, TN). RNA-seq reads were mapped to D. rerio cDNA sequences from EnsEMBL release 66.