Illumina NovaSeq 6000 paired end sequencing; RNA-seq of Wister Rat blood serum to investigate small non-coding RNAs in serum as biomarkers of tendon injury
RNA-seq of Wister Rat blood serum to investigate small non-coding RNAs in serum as biomarkers of tendon injury
Blood samples were collected via cardiac puncture immediately after death. Samples were centrifuged at 1500 g for 10 min, and serum collected and stored at -80 °C prior to analysis RNA was extracted from 100 – 200µl serum using miRNeasy kits (Qiagen, UK) according to the manufacturer's instructions. Libraries were prepared following the protocol of NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible)