ddRAD data of Anolis fuscoauratus sample PRMS590
A double restriction-site associated DNA library (ddRAD) was generated at the University of Wisconsin Biotechnology Center. Genomic DNA was digested with the PstI and MspI restriction enzymes, and the resulting fragments were tagged with individual barcodes, PCR-amplified, multiplexed, and sequenced on a single lane on an Illumina HiSeq 2500 platform (rapid run mode). The data was demultiplexed using Ipyrad v. 0.7.30.