GSM4103034: 15163X2 Aged_wt; Mus musculus; RNA-Seq
Spleens were excised from euthanized animals and single cell suspensions were prepared using frosted ends of microscope slides and filtering through 40um nylon mesh. Samples were stained with anti-CD45-APC (Biolegend) antibody at 1:500 dilution in MACS buffer (PBS supplemented with 0.5% BSA and 2 mM EDTA). Live immune cells as defined by DAPI(-)CD45(+) staining pattern were FACS-sorted by using BD FACS Aria flow cytometer. Equal numbers of sorted cells were processed for single-cell 5' RNA-sequencing via 10X Genomics. 10X Genomics single cell 3-prime gene expression v2 chemistry library prep Single-cell 3' RNA-Sequencing