WGS of Coregonus suitderi : ONT sequencing
DNA was extracted using a phenol/chloroform protocol and gDNA quality and purity was assessed using spectrophotometry, fluorometry and capillary electrophoresis. Additional purification steps were performed using AMPure XP beads (Beckman Coulter). Library preparation and sequencing were performed using Oxford Nanopore Technologies Ligation Sequencing Kit SQK-LSK109 according to the manufacturers instructions. 10g of DNA was purified then sheared to 15kb using the megaruptor1 system (Diagenode). A one step DNA-damage repair + END-repair-dA-tail procedure was performed on 2g of DNA. Adapters were then ligated to DNAs in the library. Library was loaded onto 1 R9.4.1 flowcell and sequenced on a GridION instrument at a concentration of 0,1pM for 48h.