GSM2627497: P0_CTX_CTL_NU02; Mus musculus; RNA-Seq
Brain regions were dissected out and immediately flash frozen in liquid nitrogen. Total RNA was then purified from these samples, using an mRNeasy kit (Qiagen), following the manufacturer's instructions. For P0 samples, Library construction was performed as a service by BGI America (Cambridge, MA). For P7 samples, mRNA was purified via poly(A) selection from 2ug of total RNA. Purified mRNA was then utilized to generated cDNA libraries using previously published protocols (Takahashi et al., 2015). Pooled libraries of P0 and P7 brains were separately sequenced as 50 bp single-end on BGISEQ-500, a BGI in-house sequencer at BGI or as 75 bp single-end on Illumina NextSeq 500 at UT Southwestern Medical Center respectively.