GSM3790224: BCSCsGFP [ATAC-seq]; Homo sapiens; ATAC-seq
Nuclei was extracted from samples, and the nuclei pellet was resuspended in the Tn5 transposase reaction mix. The transposition reaction was incubated at 37°C for 30 min. Equimolar Adapter1 and Adatper 2 were added after transposition, PCR was then performed to amplify the library. After the PCR reaction, libraries were purified with the AMPure beads and library quality was assessed with Qubit. The DNA samples were constructed for the library and sequenced on an Illumina Hiseq Hiseq platform.