RNA-Seq of M. norvegica_2016
After 3 days of laboratory exposure to ad libatum food at either 12 or 9 C, individuals were preserved in RNAlater. One female from the 9 C treatment was visually identified and then extracted whole using the Universal RNA Mini Kit (Quiagen). This sample was sent to University of Rochester (Rochester, NY) for sequencing and library preparation with a TruSeq kit and then sequenced on 1/2 a lane of an Illumina HiSeq 2500 as a High Output v4 125 bp PE project. Data came from the sequencing facility post trimming. They had used Trimmomatic (v.0.32) to eliminate adapter sequences (2:30:10) and removed low quality scores using a sliding window (4:20), removing both trailing and leading sequences (13) and leaving only sequences with a minimum length of 25 for downstream use.