RNA-seq analysis of wheat leaves under drought stress with NaHS pretreatment
Luohan NO.6, a Chinese bread wheat cultivar (Triticum aestivum L.) with high drought-tolerance was used as experimental materials. Wheat seeds were surface-sterilized, germinated and grew in 1/2 Hoagland solution under the conditions of 25/22 ?C (light/dark), 300 µmol m-2 s-1 light intensity, 70% relative humidity and 14 h photoperiod, the solution was changed every two days. One week later, the seedlings were pre-sprayed with 0.4 mM NaHS or water everyday for 5 d, and then transferred to 20% PEG6000 (W/V) for 24 h, therefore, four groups were included in this study: pretreated with water and normal culture group (CK), pretreated with NaHS and normal culture group (S), pretreated with water and 20% PEG6000 culture group (P) and pretreated with NaHS and 20% PEG6000 culture group (SP). After treatment, all the leaves were collected for RNA extraction. A total of eight libraries (four treatments with two replicates of each) were used for transcriptome sequencing.