Whole genome sequencing of Nymphaea thermarum
For the short insert library we used the Illumina TruSeq Library Preparation Kit with 1 g of input genomic DNA, following the manufacturers protocol. The final purified library was evaluated with an Agilent Tapestation assay, which showed a distribution of fragment sizes with a mode of ~500 bp and no signs of adapter dimer contamination. The library were sequenced on an Illumina HiSeq 2500 sequencer with v4 chemistry,; we collected 2x250 bp reads.