GSM3355462: TbetKO.Be1.d2.rep2; Mus musculus; ATAC-seq
Bulk cultures on day 2 were used for ATAC-seq Nuclei were extracted, incubated with Tn5 transposase (Illumina) for 30 minutes at 37°C and DNA samples were then purified with the MinElut Kit (Qiagen). Library amplification was performed using Nextera primers with NEBNext High-Fidelity 2X PCR Master Mix (New England Biolegends), followed by purification with the PCR Purification Kit.