GSM5165381: LTKO4; Mus musculus; RNA-Seq
Mouse knees were subjected to enzymatic digestion and CD4+ T cells were sorted from the resulting single suspension. Cells were lysed with RLT buffer and RNA was extracted using the Qiagen RNeasy microkit including on-column DNase digestion step, eluted in water and frozen at -80oC. RNA libraries were prepared for sequencing using NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina. Sequencing was carried out on the Illumina NovaSeq6000 platform using NovaSeq 6000 S2/S4 reagent kit (v1.0), 300 cycles.