GSM5176445: ANGPTL2 (A2); Homo sapiens; RNA-Seq
Cells were lysed by TRIzol reagent for extraction of RNA. RNA was prepared using RNasey Mini Kit(Qiagen p/n 74104) according to the manufacturer's recommendations. After clean-up, RNA purity was detected by NanoDrop® spectrophotometers (Thermo Fihser, MA, USA). RNA concentration was measured using Qubit® RNA Assay Kit in Qubit® 3.0 Flurometer (Life Technologies, CA, USA). RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). The clustering of the index-coded samples was performed on a cBot Cluster Generation System (Illumia, USA) according to the manufacturer's instructions. After cluster generation, the library preparations were sequenced on an Illumina Hiseq X Ten platform and 150bp pired-end module.