GSM4155112: Non-quiescent_44; Saccharomyces cerevisiae; OTHER
DNA polymerase (Roche), 0.4 ul of 100 uM “up” or “down” barcode primer pair, 0.5 ul of 10 mM dNTP, 12 ul of 25 mM MgCl2, 3 ul of 10x PCR buffer without MgCl2, and water to in a final volume of 30 ul. The primer sequences were ATGTCCACGAGGTCTCT, CCTCGACCTGCAGCGTA, CGGTGTCGGTCTCGTAG, and CCCAGCTCGAATTCATC for “up”, forward and reverse, and “down”, forward and reverse, respectively.  The amplified DNA was purified, quantitated, single-end adapters with index primers (Illumina) ligated to each sample, and single-end sequenced on a HiSeq 2500 (ARC Biotechnology Platform, University of Pretoria).