Illumina HiSeq 2000 sequencing; qiita_ptid_1142:1191.sample.15
DNA from stool samples collected from participants after consumption of both a low and high resistant starch diet, either in the context of a low or high carbohydrate diet, will be used for sequencing to identify shifts in microbial communities in response to changes in dietary composition. Microbiome patterns will be compared between responders and non-responders (upper and lower 1/3) for the main clinical outcomes of fasting and postprandial insulin and components of atherogenic dyslipidemia (triglycerides, HDL-cholesterol, small LDL particles) with adjustment for covariates including age, sex, and BMI. We will also explore the possibility that inter-individual diet-independent variation in the gut microbiome may be related to the magnitude of change in metabolic parameters in response to diet.
Illumina HiSeq V4, 515f,806rbc primers