RNA-Seq of Solanum tuberosum: germinated pollen
Pollen samples were ground in liquid nitrogen. RNA extraction was then performed using the RNeasy mini kit according to the manufacturers protocol (Qiagen GmbH, Hilden, Germany). The 250~300bp insert-size cDNA libraries were sequenced as 150nt paired-end reads, yielding 30-42 million read-pairs per sample (Novogene, Cambridge, United Kingdom)