RNAseq of homo sapiens: High Grade Upper Tract Urothelial Carcinoma Tumor Tissue: Sample WCM_UTUC_74R
RNA was extracted from frozen material using Promega Maxwell 16 MDx instrument, (Maxwell 16 LEV simplyRNA Tissue Kit (cat. # AS1280)). Specimens were prepared for RNA sequencing using TruSeq RNA Library Preparation Kit v2 or riboZero. RNA integrity was verified using the Agilent Bioanalyzer 2100 (Agilent Technologies). cDNA was synthesized from total RNA using Superscript III (Invitrogen). 