RNAseq of immunoprecipitates of Arabidopsis thaliana TERT from flower bundles: telomerase null mutant, tert, stranded library
Immunoprecipitation of telomerase was performed and total RNA was extracted using Zymo Direct-zol RNA prep kit, DNase treatment was donde in the column. The library was prepared from 500ng RNA using TruSeq Stranded Total RNA with Ribo-Zero Plant, following the ,manufacturer's instructions. The only modification was the fragmentation step was left to random priming by incubating at 65C for 5 minutes.