GSM4152233: BL1LightRep; aquifer metagenome; RNA-Seq
RNA was extracted using a bead-beating phenol-chloroform protocol modified from (Schmitt et al. 1990), shaken with a Tissuelyzer II (QIAgen) instead of a beadbeater and precipitated using 1 vol isopropanol and 0.5 vol 7.5 M ammonium acetate. After isopycnic centrifugation, RNA was converted to double stranded cDNA using the SuperScript IV First-Strand Synthesis System and SuperScript Double-Stranded cDNA Synthesis Kit (Thermofisher). Library preparation was performed using the Nextera XT kit (Illumina) according to manufacturer protocols.