GSM1844555: R20; Homo sapiens; RNA-Seq
Total RNAs from human melanocytes were prepared using PureLink RNA Mini kit (Ambion, Cat# 12183018A). The cells were lysated by addition of lysis buffer, the lysate was then homogenized using QIAshredder (QIAGEN, cat# 79654) by centrifuge at 14,000 rpm for 2 min, and then followed the manufacture's method. The RNA samples were used for RNA-seq with the range of the RIN value 8.50 ~ 10 at least. A cDNA library for deep sequencing was prepared using oligo d(T) primer, and followed the Illumina protocol (kit) for later steps.