RNAseq of lateral buds of sugarcane after 5 days planting
A sugarcane transcriptome was constructed from germinating shoot axillary buds five days after planting. Single budded setts from the sugarcane variety RB92-5345 were placed in trays with buds facing upwards, covered with moist vermiculite, and incubated at 26–30°C under greenhouse conditions. Total RNA was extracted from pooled breaking buds using a lithium chloride protocol. For the construction of RNA-seq libraries, all procedures were carried out according to Illumina’s instructions using the ‘TruSeq RNA Sample Prep v2 Low Throughput (LT)’ kit. 