RNA-seq of Drosophila melanogaster OreR;OreR: 2 hour control refed eviscerated abdomen
Total RNA was extracted from 10 male eviscerated abdomens using a phenol-chloroform extraction followed by ethanol precipitation. Concentrations and contamination was assessed by nanodrop analysis with additional quality control steps performed by Genewiz, Inc. (South Plainfield, NJ). Transcriptome sequencing was done by Genewiz using an Illumina HiSeq2500 at 6 samples per lane with 50 base pair single end reads.