RIP-seq FLAG-HEXIM1
Total RNA was extracted using Trizol. RNA quality was analyzed on a Agilent Bioanalyzer.llumina’s TruSeq Stranded mRNA Sample Prep Kit (Low throughput, random hexamer) was used to prepare the library. The final cDNA library was validated with a DNA 1000 Labchip on a  Agilent Bioanalyzer and quantified with a KAPA qPCR kit. Sample were multiplexed (by 4) on a flowcell V3  lane which provides 40-50 million single-end reads of 50 nucleotides per sample and sequenced with HiSeq2000 (Illumina).