GSM2800621: 4-24-124a-1 [mRNA]; Homo sapiens; RNA-Seq
RNA was harvested using TRIzol-chloroform. Sequencing libraries were constructed by BGI using 1 ug of total RNA to isolate mRNAs using poly-T oligo-attached magnetic beads. Following mRNA fragmentation, cDNA was generated using random primers and an adapter was ligated at the 3' end of the fragment. Products were then purified, enriched by PCR amplification, and used to create DNB-based nanoarrays by RCR. Stepwise sequencing was performed using the combinatorial probe-anchor ligation (cPAL) approach and read on the BGISEQ-500 system.