GSM3861689: Wild-type (WT) 3; Mus musculus; RNA-Seq
Intestinal crypts were isolated by mechanical disruption and EDTA treatment. Single cell suspensions were made with a 1 hr 0.25% trypsin incubation + 1mg/ml Collagenase/Dispase, DNaseI, and Q-VD-OPH. For more information, please see the reference publication. 2 Cartridges were used per genotype for a theoretical isolation of 2400 cells per genotype. Following manufacturer's protocol: SureCell WTA 3' Library Prep Kit. 68bp:Read1 and 75bp:Read2, paired-end reads. Sequenced on Illumina NextSeq 500 (hi-output mode).