GSM1206278: Pei1; Homo sapiens; RNA-Seq
Total RNA from cultured SW13 cells was extracted by direct lysis using the QIAGEN miRNeasy Mini Kit (Qiagen #217004) according to manufacturer’s instructions. Ribosome-associated RNA was obtained by using ARTseq™ Ribosome Profiling Kit (Epicenter, Illumina) and following manufacturer’s instructions. Total RNA and ribosome-assoctiated RNA fragments were further converted into DNA libraries using ARTseq™ Ribosome Profiling Kit (Epicenter, Illumina). Libraries were sequenced as 50bp paired-end reads on an Illumina MiSeq instrument.