GSM3790097: ATAC_CMP_BMT_DKO_rep2; Mus musculus; ATAC-seq
ATAC-seq experiments were performed using LSK or CMP cells obtained from WT-, SKO-, RKO-, or DKO-transplanted mice. Briefly, freshly isolated 10,000 cells were pelleted and fifty microliters of transposase mixture (25 ul of 2 x TD buffer, 2.5 ul of TDE1, 0.5 ul of 1% digitonin, and 22 ul of nuclease-free water) (FC-121-1030, Illumina; G9441) was added to the cells.  After transposition reactions at 37°C for 30 min, transposed DNA was purified using QIAGEN MinElute Reaction Cleanup kit.  Transposed fragments were PCR-amplified, and the resulting library was sequenced on Hiseq 2500.