GSM3273165: ta2-1 endosperm; Oryza sativa; Bisulfite-Seq
For whole-genome bisulfite sequencing, genomic DNA was isolated from endosperms and embryos using a QIAamp DNA Mini Kit (Qiagen). The volumes of the combined gDNA sample and unmethylated lambda DNA control were adjusted to a total volume of 80 μL using 1x TE buffer, and fragmented to 300 bp. Subsequently, fragmented DNA was end repaired and ligated to custom-synthesized methylated adapters (Eurofins MWG Operon) and a gDNA library was constructed according to the manufacturer's instructions (Illumina). Bisulfite conversion was performed using an EZ DNA Methylation-Gold Kit. The bisulfiate-converted library was amplified using KAPA HiFi HotStart DNA Polymerase (Kapa Biosystems).