RNA-Seq of peripheral blood: female T2DM patient
RNA-sequencing libraries were prepared using the rRNA-depleted RNA by NEBnext ultra RNA library prep kit. After that, the quality of libraries were determined using the Agilent 2100 system (NanoDrop ND-1000) and accurately quantified by quantitative real-time PCR (qRT-PCR). Lastly, the libraries were pooled according to the requirements of effective concentration and data volume, and then sequenced on Illumina Hiseq 2000 platform.