RNA-Seq of mixed sample :B73 and Setosphaeria turcica
Total RNAs were extracted from the pathogen S. turcica and maize leaves using the Trizol (Invitrogen) method according to the manufacturers instructions. A total amount of 4 g RNA per sample was used as input material for the RNA sample preparation. Sequencing libraries were generated using Illuminas 'TruSeq Stranded RNA Sample Prep kit following manufacturers recommendations. The libraries were pooled in equimolar concentration, quantitated by qPCR and sequenced on five lanes for 101 cycles on a HiSeq2500 platform using a TruSeq SBS sequencing kit version 4. Fastq files were generated and demultiplexed with the bcl2fastq v1.8.4 Conversion Software (Illumina) and 100 nt reads were generated.