GSM4155484: Cortex_Dnmt3a-WT_Rep4; Mus musculus; RNA-Seq
For miR-29Nestin mice, total RNA were extracted from the cortex of P40 mice using RNeasy Mini Kit (Qiagen). For Dnmt3ami5 mice, total RNA were extracted from the cortex of 4.5 months old mice using Direct-Zol RNA mini-prep kit (Zymo Research, #R2051). For mirR-29Nestin mice, stranded mRNA libraries were prepared with TruSeq Stranded mRNA Library Prep Kit (Illumina). For Dnmt3ami5 mice, stranded mRNA libraries were prepared using the KAPA stranded mRNAseq kit (KAPA Biosystems) Paired end sequencing (50 cycles) was performed on an Illumina HiSeq 2500 v4 (high output) or Illumina HiSeq 4000 (high output)