GSM2970513: CD31-CD45-CD90+ cells [cd90 cell16]; Homo sapiens; RNA-Seq
Separated papillary and reticular dermis samples were transferred to lysis buffer containing 2-mercaptoethanol (PureLink RNA micro scale kit, Invitrogen) and homogenized for 2 minutes using a mechanical homogenizer (Polytron, Kinematica). Subsequent RNA extraction was performed using the PureLink micro kit according to the manufacturer's instructions. Library preparation was performed with SmartSeq2 followed by the Nextera XT protocol (Illumina). Sequencing was performed on the Illumina HiSeq 2000 or HiSeq 2500 with TruSeq SBS v3 chemistry.