platycodon IT 209935 transcriptome
Total RNA was extracted from 1-month-old leaves of 3 platycodon accessions with the RNeasy®Plant Mini Kit (QIAGEN, CA, USA), using the protocol described by the manufacturer. The Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA) was used to check RNA concentration and integrity, as well as for possible contamination by ribosomal RNA. Barcoded cDNA libraries for multiplex sequencing were prepared from 5 g total RNA using a modified TruSeq method (Zhong et al., 2011). The libraries were then sequenced at the Cornell University Life Sciences Core Laboratories Center using the Illumina HiSeq2000 system