Illumina-seq of ciliate: V9 region of 18S rDNA
DNA extraction with Qiagens DNeasy Blood & Tissue Kit. PCR amplification with V9 specific primers (V9F: 5-GTACACACCGCCCGTC-3; Lane, 1991; V9R: 5-TGATCCTTCTGCAGGTTCACCTAC-3; Medlin et al., 1988). Sequencing library construction for Illumina sequencing with respective Illumina kit.