GSM1568187: Cnp1_FLAG_5min_150salt_IP_(20140731_14); Schizosaccharomyces pombe; ChIP-Seq
Cells were grown in YES media. Nuclei were prepared following previously published protocol (PMID: 1988457) and subjected to MNase digestion. Resulting chromatin solution was needle extracted and the insoluble pellet was solublilized with 150 mM NaCl containing extraction buffer (0.1% Triton X-100, 10 mM phosphate buffer, 0.75 mM EDTA, 150mM NaCl) for 4 hrs at 4 degree Celsius. The solubilized chromatin was subjected to native chromatin immunoprecipitation as described previously (PMID: 22184235).