PacBio RS II whole genome sequencing of the marine Sulfitobacter sp. D7
Fragmented DNA was enzymatically repaired and ligated to a PacBio adapter to form the SMRTbell Template. Templates larger than 10kb were BluePippin (Sage Science) size selected, depending on library yield and size. Templates were annealed to sequencing primer, bound to polymerase, and then bound to PacBio MagBeads and SMRTcell sequenced.