Illumina HiSeq 1000 paired end sequencing; Otx2 binding to Nanog promoter protects embryonal stem cells from corruption of naive pluripotency and is required at morula stage for inner-cell mass differentiation of the epiblast
Otx2 binding to Nanog promoter protects embryonal stem cells from corruption of naive pluripotency and is required at morula stage for inner-cell mass differentiation of the epiblast
EpiSCs were induced from ESCs on serum-coated dishes in N2B27 medium supplemented with activin A and Fgf2. RNA was extracted with Trizol (Invitrogen) Indexed sequencing libraries were generated from 1ug RNA, using the TruSeq Stranded Total RNA Sample Prep Kit with Ribo-Zero ribosomal RNA reduction chemistry (Illumina, San Diego, CA), according to the manufacturer’s instructions.