GSM3406852: IC-H455R; Homo sapiens; RNA-Seq
percutaneous muscle biopsy was performed and sampels was flash frozen in liquid nitrogen.  Total RNA was extracted using Qiagen Rneasy Midi Kits per manufacturer instructions. Library for RNA-Seq was prepared according to KAPA Stranded mRNA-Seq poly(A) selected kit with 201-300bp insert size (KAPA Biosystems, Wilmington, MA) using 250 ng total RNA as input. Final library quality and quantity was analyzed by Agilent Bioanalyzer 2100 and Life Technologies Qubit3.0 Fluorometer. 150 bp paired end reads were sequenced on Illumina HighSeq 4000 (Illumnia Inc., San Diego, CA).