Next-generation Seq. of WZ_Cpf1 edited samples: human genome
After collecting cells, genomic DNA was purified. Amplicons for next-generation sequencing were generated using two rounds of PCR. Equal amounts of the PCR amplicons were subjected to paired-end read sequencing using the Hiseq 2500 platform (Illumina, San Diego, CA, USA) at Novogene (Tianjin, China). Adaptors and low-quality reads were removed from the resulting 150-bp paired-end reads using Trimmomatic (version 0.36). Reads were then mapped to the template using Bowtie2 (version 2.3.3).