GSM1357496: Sample_HYT_4-5; Rattus norvegicus; RNA-Seq
Trizol RNA Extraction reagent (Life Technologies) and QiaShredder (Qiagen) were used to lyse and homogenize whole hypothalamus. An RNEasy kit from Qiagen was then used to extract RNA. An Agilent 2100 Bioanalyzer was used to assess RNA purity and quality. One microgram of total RNA was then used to build mRNA sequencing libraries using standard Illumina protocols