GSM4704815: RD-ES; Homo sapiens; ChIP-Seq
RD-ES cells were chemically crosslinked and sonicated to generate fractionated genomic DNA (gDNA) with fragment sizes of 100–500 bp. Sonicated chromatin was incubated with anti-HDGF antibody (Abcam, ab128921) coupled to Protein G beads (Dynabeads) overnight at 4°C. For library preparation, the DNA fragments were blunt end-repaired, 3'-dA-tailed, and ligated to the Illumina adaptors according to the manufacturer's instructions (Illumina DNA Sample Prep kit). After adapter ligation, DNA was PCR amplified, size selected (usually 100-300bp, including adaptor sequence), and sequenced on an Illumina HiSeq 2000 Genome Analyzer.