GSM3409440: Dam-Sd Rep1; Drosophila melanogaster; OTHER
CNSs were treated with protK, RNase A followed by phenol chloroform DNA extraction. DNA was ethanol precipitated, washed and dissolved, followed by DpnI digestion, DamID adaptors ligation, DpnII digestion and PCR amplification. MePCR product was sonicated using a Covaris S2 device to generate fragments of 100-500bp, with an average of 300bp. DNA was purified using Agencourt AMPure beads purification. Illumina sequencing libraries were prepared using KAPA Hyper prep kit (Kapa Biosystems) and 300ng starting material. Five to seven cycles of PCR were performed.