GSM4931883: H-4S-1a_ATAC-seq; Mus musculus; ATAC-seq
Oct4-GFP+ cells were sorted directly into lysis buffer by flow cytometry from Oct4-GFP/+ embryos at indicated developmental stage Sorted cells were resuspended in transposase reaction mixture (25 μL of 2X Tagmentation DNA Buffer [TD buffer; Illumina, Cat#FC-121-1030], 2.5 μL Tn5 enzyme [Illumina, Cat#FC-121-1030], 0.5 μL 1% digitonin [Promega Cat#G9441], and 22 μL water), incubated for 30 min at 37°C Tagged and transposed DNA fragments were purified using MinElute Reaction Cleanup kit (Qiagen) Genomic DNA libraries were prepared for sequencing using standard Illumina protocols Nextera DNA Library Prep kit