GSM2627268: CD4_resting_Biotin-4C IL2RA_Nla_III_rep; Homo sapiens; OTHER
The DNA is extracted by phenol-chloroform extraction and ethanol precipitation as routine molecular biology precedue. The restriction enzymes used are DpnII and NlaIII. The libraries are prepared according to reference: Davies, J. O., et al. (2016). "Multiplexed analysis  of chromosome conformation at vastly improved sensitivity." Nat Methods 13(1): 74-80.) with the following differences: we fixed the cells with 1% formaldehyde (Thermo Scientific prod# 28908)-containing culture media at room temperature for 5 minutes with gentle shaking, followed by adding glycine to 0.125M to quench the reaction for 5min and washing twice with PBS. The hybridization is done without using the blockers.