GSM3273137: Untreated Rep 3 -A-IP; Escherichia coli; ChIP-Seq
DNA was extracted from resulting supernatant with phenol/chlorophorm method followed by ethanol precipitation. Mock controls (-IP) were made both for +A and for -A: 100 μl aliquots of lysates obtained after sonication were deproteinized and DNA was purified as described earlier. The procedure described gives a quartet of samples (+A+IP, +A-IP, -A+IP, -A-IP), where +A-IP, -A+IP, and -A-IP serves as controls for gyrase poison action and immunoprecipitation. Sequencing libraries were prepared with Accel NGS 1S kit (Swift Bioscience) in accordanse with manufacturer's protocol.