GSM2970850: PrefrontalCortex_A9-2; Mus musculus; RNA-Seq
Approximately 30 mg of frozen brain tissue was then pulverized and RNA was extracted using Qiagen miRNeasy kits according to the manufacturer's protocol For each sample, RNA integrity number (RIN) values were quantified using an Agilent Bioanalyzer. RNA sequencing libraries were prepared using TruSeq Stranded mRNA Library Prep kits using polyA selection (Illumina). Libraries were barcoded and randomly pooled in sets of 24.