GSM4392021: Imp1 KO_rep2; Homo sapiens; RNA-Seq
RNA extraction was performed using TRIZOL according to manufacturer's protocol. Poly-A RNA was enriched using oligo(dT) beads. RNAs are fragmented randomly by adding fragmentation buffer. cDNA is synthesized by using random hexamers primer followed by second stramd synthesis. Strand-specific double-stranded cDNA libraries are completed by size selection (250-300bp) and PCR enrichment. Completed libraries are sequenced on an Illumina HiSeq platform. poly-A RNA-Seq