16s rrna gene sequencing of ileal content: broiler chickens
The variable V4 16S rRNA region of genomic DNA was amplified by PCR using the primers 515F (5-GTGCCAGCMGCCGCGGTAA-3) and 806R (5-GGACTACHVGGGTWTCTAAT-3), which was followed by normalization and measurement of quality and quantity of PCR products. Illumina MiSeq 2  250 bp paired-end sequencing was used to sequence pooled amplicons.