GSM1569870: WT FI_SIRT1_ChIPSeq2; Mus musculus; ChIP-Seq
For ChIPSeq, lysates were cleared from sonicated nuclei and used for immunoprecipitation. For RNASeq, RNA was extracted according to TRIzol protocol (invitrogen). For ChIPSeq, cells were cross-linked in 1% formaldehyde and lysate to extract nuclei. The chromatin was sonicated to 250 bp fragments. Histone-DNA complexes were isolated with H4K16ac antibody (Millipore, CS204361) or Sirt1 antibody (Upstate, 07-131) Purified DNA was processed according to manufacturer's protocol. For polyA RNA-Seq, Illumina protocol was followed.