GSM1846997: Funiculus Cortex 1; Brassica napus; RNA-Seq
RNA was extracted from LMD-collected tissues using the Ambion RNA Aqueous microkit (Life Technologies, Carlsbad, CA, USA) according to manufacturer's instructions cDNA was amplified with the Ovation RNA-seq V2 kit (NuGEN, San Carlos, CA, USA). The cDNA libraries were then further refined using the Illumina TruSeqTM RNA Sample Preparation v2 kit (Illumina, San Diego, CA, USA) using the low-throughput protocol according to manufacturer’s instructions