GSM3895469: WT2; Vibrio cholerae; RNA-Seq
For in vitro RNA preparations, the wild type strain and degS deletion mutant strains were grown to mid-exponential phase (OD600 of ~0.6) in LB liquid medium. Total RNA was extracted from the wild type and degS deletion mutant using TRIzol Reagent (TaKaRa, Japan). After ensuring quality control, the cDNA libraries were prepared, and sequencing was performed by NovelBio Bio-Pharm Technology Co. Ltd. (Shanghai, China). Ion  Proton Sequencing Protocol RNA-Seq useing Ion Torrent Proton,Torrent Suite Software and Torrent Server for  base calling