GSM4101791: V36_1; Vibrio parahaemolyticus; RNA-Seq
Total RNA was then isolated from C. elegans containing bacteria using the mirVana kit (Ambion) following the user manual. The isolated total RNA was treated with DNase I (Fermentas) (0.25 U/1 μg of RNA) for 30 mins, followed by purification with ethanol. The Ribo-Zero Magnetic Gold Kit (Epidemiology) from Epicentre/Illumina was used to removed rRNA in the samples following the manufacturer's instructions. cDNA libraries for Illumina sequencing were constructed with NEBNext Ultra Directional RNA Library Prep Kit for Illumina (E7420) following the manufacture instruction. For organism dual RNA-Seq, ~ 100 ng rRNA-depleted RNA were used for cDNA library preparation