Metastatic Sample  6 (Normal)
Genomic DNA from frozen tissue was extracted using the Qiagen Gentra Puregene Tissue Kit (Qiagen). DNA was prepped with the KAPA Hyper kit (Roche) and sheared using a Covaris LE220 ultrasonicator targeting 200bp. Libraries were hybridized to SeqCap EZ Choice probes of the 50 Mb Human UTR Design (Roche), and sequenced on a HiSeq 2500 (Illumina) using a PE100 in high-output mode. Image analysis and base calling were performed using Illumina's Real Time Analysis v 1.18.66.3 software, followed by demultiplexing of indexed reads and generation of FASTQ files, using Illumina's bcl2fastq Conversion Software v1.8.4.