intestine and lung
Total RNA was extracted from RNAlater-preserved tissue (intestine, lungs, liver, muscle) of the same individual used for the genomic sequencing using PureLink RNA Mini Kits (Life Technologies, Carlsbad, CA), following an adapted protocol from Gunter et al. (2013). RNA samples were quantified with a Qubit 2.0 fluorometer (Life Technologies, Carlsbad, CA) and quality was assessed with a 2200 Tapestation (Agilent, Santa Clara, CA). The libraries were prepared with Illumina TruSeq Total Stranded RNA-seq protocol and sequenced together on one lane of Illumina HiSeq4000 (150 bp paired-ends).