GSM3272787: RNA-seq_Lung_control_1; Mus musculus; RNA-Seq
Total RNA was extracted from mouse kidney, liver, lung and spleen using TRIzol RNA extraction (Thermo Fisher Scientific).  After phase separation, RNA was purified using the PureLink RNA Mini Kit (Thermo Fisher Scientific).  Purified total RNA sample was treated with DNase I (TURBO DNA-freeTM kit, Ambion Inc.) to remove traces of DNA.  RNA library was prepared using NEBNext Ultra RNA library prep kit (Illumina) according to manufacturer's instruction.  Total RNA starting with 1ug was poly-A selected, fragmented by random priming and then converted to cDNA using ProtoScript II reverse transcriptase.  The cDNA was then end-repaired, adenylated and ligated with Illumina sequencing adaptors.  Libraries from all samples were pooled and sequenced using an Illumina HiSeq 4000 with 50-bp pair-end read.