Huso huso male 1 genomic sequencing
DNA-seq libraries have been prepared according to Illuminas protocols using the Illumina TruSeq Nano DNA HT Library Prep Kit. Briefly, DNA was fragmented by sonication, size selection was performed using SPB beads (kit beads) and adaptators were ligated to be sequenced. Library quality was assessed using a Advanced Analytical Fragment Analyzer and libraries were quantified by QPCR using the Kapa Library Quantification Kit. DNA-seq experiments have been performed on an Illumina NovaSeq6000 using a paired-end read length of 2x150 pb with the Illumina NovaSeq6000 Reagent Kits.