GSM2872715: CM150-post [RNA-Seq]; Homo sapiens; RNA-Seq
RNA was extracted from the cell lines using RNeasy Plus mini prep kit (QIAGEN, Limburg, Nether- lands), quantified using a Nanodrop (Thermo Scientific, Wilmington, DE) Total RNA was isolated with RNeasy Mini Kit (Qiagen) following manufacturer's protocol. Two rounds of RNase-free DNase I digestion (Qiagen) was performed to eliminate genomic DNA during the extraction process. RNA concentrations were determined using NanoDrop 2000 (Thermo Scientific). The quality and integrity of the RNA was determined using the RNA 6000 Pico chip on 2100 Bioanalyzer (Agilent Technologies). The median RNA integrity number (RIN) for the 12 samples was 8.0. RNA libraries were constructed using 1 µg of total RNA with TruSeq stranded mRNA Sample Preparation kit (Illumina, USA) following the manufacturer's protocol.