GSM4154617: NF50_3 [RNA-seq]; Xenopus tropicalis; RNA-Seq
We isolated total RNA from tadpole brain using Tri Reagent (Sigma-Aldrich, St. Louis, MO) and the Direct-zol RNA kit (Zymogen) following the manufacturers' instructions. The quality of the RNA was analyzed using a Bioanalyzer. Total RNA libraries were prepared by the University of Michigan DNA Sequencing Core.