RNA-Seq of M. norvegica_2016
After 3 days of laboratory exposure to ad libatum food at either 12 or 9 C, individuals were preserved in RNAlater. Tail tissue from 5 individuals each were pooled and extracted using the Universal RNA Mini Kit (Quiagen). 5 samples from each of the temperature treatments was sent to University of Rochester (Rochester, NY) for sequencing and library preparation with a TruSeq kit and then sequenced on 1 lane (10 total samples) of an Illumina HiSeq 2500 as a High Output v4 125 bp PE project. Data came from the sequencing facility post trimming. They had used Trimmomatic (v.0.32) to eliminate adapter sequences (2:30:10) and removed low quality scores using a sliding window (4:20), removing both trailing and leading sequences (13) and leaving only sequences with a minimum length of 25 for downstream use.