GSM2627469: N5496_delta-dim1_Bisulfite; Neurospora crassa; Bisulfite-Seq
Cultures were grown in Vogels medium and 1.5% sucrose (standard minimal medium), shaking for 48 hours at 32oC. Genomic DNA was isolated using a protocol modified from (Oakley et al, 1987).  Genomic DNA (50ng) was treated with bisulfite using the EZ DNA Methylation LightningTM kit (ZYMO Research) per the manufacturer's protocol. Bisulfite-Seq Libraries were prepared for sequencing using the EpiGnomeTM Methyl-Seq Kit (Epicentre cat #EGMK91324) and EpiGnomeTM Index PCR Primers (Epicentre cat# EGIDX81312), the library was purified with Agencourt AMPure XP beads (Beckman Coulter), quantified using a Qubit HS Assay (Life Technologies), visualized on the Fragment AnalyzerTM (Advanced Analytical), and sequenced on a Illumina HiSeq 2000