GSM4103153: 11610-11; Mus musculus; RNA-Seq
E13.5 whole brains were dissected and homogenized in 500ul of RIPA buffer with RNAse inhibitors. 250ul of homogenate was added to 750ul of Trizol LS and stored at -80C until RNA extraction. RNA was extracted using the Zymo clean and concentrator-5 kit with on column DNAse digestion and eluted in 30ul of RNAse free water. 1ug of RNA was used as input for the rRNA depelition kit (human/mouse/rat) (NEB#E6310) and then libraries were prepped using the NEBNext Ultra II RNA Library prep kit for Illumina (E7770L)