WGS of Cryptococcus neoformans:HIV-u-98
Use SDS or STE method to extract the genomic DNA of the sample, then use agarose gel electrophoresis to detect the purity and integrity of the DNA, and use Qubit for quantification. The DNA samples that passed the electrophoresis test were randomly broken into fragments with a length of approximately 350 bp using a Covaris ultrasonic disruptor. After processing the DNA fragments, use NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, USA) to complete the library preparation through end repair, A-tailing, sequencing adapters, purification, and PCR amplification.