GSM2800601: OL; Homo sapiens; ncRNA-Seq
Blood was centrifuged at 3000 rpm for 10 min to separate plasma and stored at −80°C. For small RNA sequencing, total RNA was extracted from 3 ml of pooled plasma using a combined version of phenol–chloroform extraction followed by column purification. Library was constructed by TruSeq Small RNA Preparation Kits (Illumina Inc, CA,  USA) according to the manufacturer’s instructions.