Amphimedon queenslandica cell-type CEL-Seq
Sponge cells were dissociated using a 20 _m mesh and/or by squeezing tissue with forceps. Cells of interest were collected manually and pooled using an inverted microscope (Nikon Eclipse Ti microscope) and a micromanipulator (MN-4, Narishige) connected to CellTram Oil (Eppendorf). The CEL-Seq2 protocol (Hashimshony, et al. 2016) was used to amplify and sequence, using CEL-seq multiplexing barocdes.