GSM2875270: C251_MEP_neg; Mus musculus; RNA-Seq
E14.5 Runx1 P1-GFP::P2-hCD4/+ fetal livers (from two litters) were isolated, pooled and manually disaggregated in DMEM supplemented with 10% Fetal Bovine Serum. They were then stained with fluorescently conjugated antibodies in preparation for FACS sorting.  Single P2-hCD4-/+ MEPs and CMPs were sorted into 384-well plates containing lysis buffer and snap frozen. cDNA was prepared using a Mantis platform (Formulatrix) and quantified with quantIT picogreen reagent (Thermo Fisher). Dual indexed sequencing libraries were prepared from 0.1ng cDNA using an Echo525 automation system (Labcyte) in miniaturized reaction volumes.  The library pool was quantified by qPCR using a Library Quantification Kit for Illumina sequencing platforms (Kapa Biosystems).