GSM3786911: Monocytes from non-small lung cancer patient p3; Homo sapiens; RNA-Seq
Cell pellets containing 500,000 - 1 million cells were lysed in 300 ul of Tri- Reagent (Sigma, St. Louis, MO) and RNA was extracted using the Direct-Zol RNA Mini-Prep Kit (Zymo Research, Irvine, CA), including an on-column DNAseI treatment. 3' mRNA-seq libraries were generated from DNAseI treated total RNA using the QuantSeq FWD Library Preparation kit (Lexogen, Vienna, Austria), according to manufacturer's directions.