GSM1846400: MTHFR_KO_Saline_1; Mus musculus; Bisulfite-Seq
Lungs were perfused with phosphate buffered saline then removed and flash frozen in liquid nitrogen.  DNA was extraced using Qiagen's Dneasy Columns.  Agilent's SureSelect Methyl-Seq Target Enrichment System for Illumina Multiplexed Sequencing kit was used with 4 ug of DNA for the construction of sequencing libraries. Methyl-seq libraries were preapared for sequencing using SureSelect Human Methyl-Seq Protocol Version B using SureSelect Methyl-Seq Reagent Kit and Mouse Methyl-Seq Capture Library.