GSM3581775: Nxt1 DG05102/Nxt1 Z2-0488_rep2; Drosophila melanogaster; RNA-Seq
3rd instar stationary larvae were dissected in a magnetic chamber and all internal tissues were removed. The larval carcasses were put in PBS and flash frozen in liquid nitrogen. RNA was extracted with Trizol reagent. TruSeq Stranded mRNA Library Prep (Illumina) was used with 0.5ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols