GSM5221256: 0h_2; Mus musculus; RNA-Seq
RNA was extracted using RNeasy Kit (QIAGEN) following the manufacturer's instructions with an additional on-column DNase treatment using the RNAse-Free DNase set (Qiagen). RNA was quantified using Qubit 2.0 (Thermo Fisher Scientific). Libraries were prepared using the TruSeq stranded mRNA kit (Illumina) and sequenced in a HISeq 3000 (Illumina)