GSM4704542: 1177; Homo sapiens; RNA-Seq
Surgical material was collected at our institution at the time of surgery with consent (COMIRB 95-500). Samples were collected into PBS for subsequent scRNA-seq analysis and snap frozen for bulk-tumor methylome analysis. ScRNA-seq samples were rapidly dissociated into single cells using a mechanical process as described previously, viably cryopreserved and banked at <80oC for later use. (Reference Griesinger, A. M. et al. Characterization of distinct immunophenotypes across pediatric brain tumor types. J Immunol 191, 4880-4888, doi:10.4049/jimmunol.1301966 (2013).) 3' end 10x Genomics library prep (v2 and v3)