PacBio Sequel of Fusarium langsethiae str. Fe2391
To obtain long sequence fungal DNA, the protocol from Bacha (2015) was used. Briefly, 3-day-old colonies of F. langsethiae Fe2391 (Torp and Nirenberg, 2004) grown on Potatoes Dextrose Agar (PDA) were harvested and frozen in liquid nitrogen. The mycelia were incubated for 10 min at 50C in a modified lysis buffer (1% of hexadecyl-trimethyl-ammonium bromide, 100mM pH8 EDTA, 1.4 M NaCl, 20mM pH8 Tris-HCl). The DNA was then extracted 3 times in phenol:chloroform prior to precipitation in isopropanol. The pellet was resuspended with 25U of RNAse prior to sending for sequencing. The DNA size (~20kb) was vali-dated by gel electrophoresis. The samples were sent to Novogene, China, which generated raw sequencing data using the PacBio Sequel platform.