GSM4154952: 4RARA38_RV_S39; Rattus norvegicus; RNA-Seq
Fresh frozen tissue (~25-50mg) from rat lung or heart RV was ground with a mortar and pestle kept on dry ice. The pulverized tissue was homogenized in 1ml Trizol using the Polytron homogenizer at 20krpm for 1 minute on ice. Total RNA was isolated from the Trizol homogenate after high speed centrifugation (20 minutes, 4C) using Zymo Direct-zol column procedure and included on-column DNase digestion. RNA quality and integrity was determined by UV spectrum ratios and Agilent BioAnalyzer Tapestation. Libraries were prepared using the KAPA mRNA Hyperprep Dual Index Kit, and both tissues' libraries were mixed and run on the Illumina HiSeq4000 in one flowcell across five lanes.