GSM4392097: Week11_EGFR;p53; Mus musculus; OTHER
Genomic DNA was isolated from bulk tumour-bearing lung tissue from each mouse. Three cell lines with a defined sgID-BC added at 5x10^5 cell/sample were added to each mouse lung sample prior to lysis to enable the calculation of the absolute number of neoplastic cells in each tumour from the number of sgID-BC reads. Q5 High-Fidelity 2x Master Mix was used to amplify the sgID-BC region from 32 µg of genomic DNA using unique dual-indexed primers.