GSM3406671: GRMut3; Danio rerio; Bisulfite-Seq
Whole brains were dissected and genomic DNA was extracted using Qiagen Dneasy Blood and Tissue Kit (Cat No./ID: 69504) according to the manufacturer's instructions. Library prep protocol was optimised at GATC Biotech based on the NEBNext Ultra I DNA Lib Prep Kit for Illumina. This included: fragmentation by sonication, end repair, a-tailing, ligation of methylated adapters, bisulfite conversion, index PCR, size selection by extraction from agarose gel (band at ~350 bp)