GSM5221755: LEC_II4_rep2; Homo sapiens; RNA-Seq
RNA was extracted using High Pure RNA Isolation Kit (11828665001, Roche). RNA quality was evaluated using the Agilent 2100 Bioanalyzer System and associated RNA 6000 Nano assay (Agilent Technologies). Samples with an RNA integrity number of less than 8 (RIN < 8) were discarded. cDNA libraries were generated from 500 ng total RNA of each sample using Truseq® stranded mRNA Sample Preparation Kits (Illumina, CA, USA) following manufacturer's instructions. Libraries were validated on a Bioanalyzer DNA 6000 nano chip and quantified by qPCR using the KAPA Library Quantification Universal kit (Roche)