GSM3288593: WT12_HiC_1 reanalysis; Saccharomyces cerevisiae; Hi-C
DNA was extracted by pestle and mortar liquid Nitrogen grinding and SDS solubilization.  Triton X-100 was used to quench the SDS before O/N digestion with HindIII.  Digested DNA ends were then biotinylated and blunt-end ligated under dilute conditions.  Unligated biotinylated ends were removed with T4 DNA Polymerase and crosslinks were reversed.  The DNA was concentrated using a Zymogen Clean and Concentrate Kit-5 and sonicated in a Diagenode Bioruptor. Biotinylated ligation junctions were capture by Streptavidin coupled Dynabeads and libraries were constructed on the beads using Nextera Mate Pair Reagents (Illumina).