GSM5159103: sn C57BL/6J Aged 4V rep1; Mus musculus; RNA-Seq
For embryonic single cell sequencing, whole cells were isolated by treatment of lateral, third and fourth ventricle choroid plexus tissue with Collagenase II (Gibco), TyrpLE (Life Technologies) and trituation. Healthy cells were then FACS sorted using LIVE/DEAD stainig kit (ThermoFisher). For nuclei isolation from each ventricle across all ages, we first minced the tissue in CST Lysis buffer. Nuclear suspension diluted with ST buffer and samples were filtered and collected by centrifuation followed by filtration before loading.