GSM1844756: CHL-1 + CRISPR rRNA removed; Homo sapiens; OTHER
Cells were washed with ice-cold PBS, lysed on ice with cyclohexamide-containing buffer Sequencing library was prepared as described in Ingolia NT, Brar GA, Rouskin S, McGeachy AM, Weissman JS. The ribosome profiling strategy for monitoring translation in vivo by deep sequencing of ribosome-protected mRNA fragments. Nat Protoc. 2012, 7(8):1534-50