NextSeq 500 paired end sequencing; Generation of a new mouse model of glaucoma characterized by reduced expression of the AP-2ß and AP-2Δ
Generation of a new mouse model of glaucoma characterized by reduced expression of the AP-2ß and AP-2Δ
Genomic DNA from mutXDH transgenic Mouse (XdhTg). Transgenic mice were originally generated in the B6D2F1 hybrid strain (C57BL/6 X DBA2) and subsequently stabilized in C57BL/6 background  by over 10 successive rounds of inter-breeding. Liver dissected from mutXDH transgenic mouse (XdhTg) DNA from liver was extracted using DNA-Mini Kit (Qiagen) and fragmented using Bioruptor (Diagenode). 380 ng DNA in 100 microL were subjected to sonication with the following parameters: power setting low, cycle 30sec on/ 90sec off, time 8 minutes in ice-cold, degassed water, resulting in average 550 bp size fragments.