GSM3722433: D36.1; Siansivirga zeaxanthinifaciens CC-SAMT-1; RNA-Seq
Total RNA was extracted from cell pellet using TRI reagent solution (Ambion) according to manufacturer's instructions. Ultrapure DEPC water and 1-bromo-3-chloropropane were purchased from Protech and Sigma-Aldrich, respectively. Residual DNA was removed by using TURBO DNase (Ambion) according to the manufacturer's instructions. The quality and quantity of RNA were assessed using a NanoPhotometer (Implen). DNA-free RNA was subjected to ribosomal RNA depletion using the MicrobeEXPRESS kit (Ambion) according to manufacturer's instructions. A complementary DNA (cDNA) library of the messenger RNA was prepared by using TruSeq® Stranded mRNA Sample Preparation kit (Illumina, San Diego, CA) according to the manufacturer's instructions. The cDNA library was generated after reverse transcription and 15 cycles of PCR using primers analogous to the adapters.