RNA-seq of ESB
Total RNA purity and degradation were checked on a 1% agarose gel. RNA integrity was assessed using the RNA Nano 6000 Assay Kit on the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Complementary DNA (cDNA) libraries were constructed using the TruSeq RNA Sample Preparation kit v2 (Illumina, USA) following previously described procedures (Aedo et al., 2015). Libraries were sequenced (2  150 bp) using the Illumina platform.