Bacterial V3-V4 16S rRNA amplicons from aquatic community of King George Island pond
A variable region, V3-V4, of the 16S rRNA gene was amplified using 5-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG-3 and 5-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGCACHVGGGTATCTAATCC-3 primers and KAPA HiFi DNA Polymerase.