GSM3272748: Phf6-WT2; Mus musculus; RNA-Seq
Total RNA was extracted using Qiagen RNeasy Mini Kit (cat number 74004) NEBNext Ultra RNA Library Prep Kit for Illumina was used for cDNA synthesis and library preparation per manufacturer's specifications. Pooled libraries were sequenced on an Illumina HiSeq platform using a 2x150 Paired End (PE) configuration to generate approximately 58 million reads per sample.