ChIP-seq_MPP_input
Committed erythroid and myeloid progenitors/precursors (EPP and MPP) were isolated as pools of CD34lowCD36high and CD34-CD13+ populations, after induction for five days in culture in the presence of SCF, IL3 and either Epo or G-CSF, respectively Chromatin was prepared after cross-linking for 10’ at RT with 1% formaldehyde-containing medium, using truChIP™ High Cell Chromatin Shearing Kit with SDS Shearing Buffer (Covaris). Nuclear extracts were sonicated to obtain DNA fragments averaging 200 bp in length and  immunoprecipitated the equivalent of 107 cells overnight with 10 μg of rabbit antibodies against H3K4me1, H3K4me3 and H3K27ac, as previously described (Cattoglio et al., 2010b; Cui et al., 2009).  Libraries were prepared according to Illumina's instructions accompanying  the standard protocol of Illumina ChIP-seq DNA sample preparation kit (IP-102-1001, Illumina Inc.).