GSM4103400: CLR4 parental; Homo sapiens; RNA-Seq
To ensure minimal contamination from mouse stromal or blood cells during RNA sequencing, we performed flow cytometric cell sorting of the PDX cell suspension after it had been processed through the magnetic-based mouse cell depletion kit (130-104-694, Miltenyi).  Single cells that bound an APC-conjugated anti-human CD326 antibody (324208, BioLegend) and did not bind to a FITC-conjugated anti-mouse H-2Kd antibody (116606, BioLegend) were positively selected and considered to be PDX colorectal cancer cells.  RNA was isolated from these double-sorted CRC PDX cells (37500, Norgen), ribosomal RNA was removed (MRZH11124, illumina), and the samples were prepared for RNA-sequencing using script-seq V2 (SSV21124, illumina).