GSM4153635: Mature leaves_Day 8_-Mg, rep2; Arabidopsis thaliana; RNA-Seq
Mature leaves, expanding leaves and roots (ca. 10 mg) were collected, flash-frozen and lysed with lysis/binding buffer containing Sodium dodecyl sulphate. mRNA was isolated from the lysate by magnetic bead purification. A strand-specific RNA-Seq library was constructed according to Townsley et al., Frontiers in Plant Science, 2015. Briefly, mRNA was fragmented and primed with 3' primer, cDNA was synthesised, and strand-specific 5' adapter sequence was added and incorporated. The average fragment length of the constructed library was approximately 400 bp. All the libraries were pooled and subjected to 50 bp single-end sequencing with Illumina HiSeq 2500.