GSM1357533: Th17 cells treated with Digoxin (10uM) [RNA2-2]; Mus musculus; RNA-Seq
RNA was extracted with RNeasy Plus kits (Qiagen) RNA was first subjected to FastAP Thermosensitive Alkaline Phosphatase (Thermo Scientific), followed by a 3′ ligation of an RNA adaptor using T4 ligase (New England Biolabs). Ligated RNA was reverse transcribed using AffinityScript Multiple Temperature Reverse Transcriptase (Agilent), and the cDNA was subjected to a 3′ ligation with a second adaptor using T4 ligase. The single-stranded cDNA product was then amplified for 9-14 cycles in a PCR reaction.