delta_gcn4_SL_NSAA_Rep1
Δgcn4 – a GCN4 deletion mutant of S. cerevisiae CEN.PK was grown in YP media containing Glucose has been shifted to the minimal media containing all non-sulphur containing amino acids and mRNA was extracted using hot phenol method and mRNA libraries were prepared using TruSeq RNA library preparation kit V2 (Illumina)