CRISPR-edited WGS fastq
Total genomic DNA was extracted from 0.5 cm tails from the CRISPR-edited Zkscan1+/- mouse and three control mice using the HiPure Tissue DNA Mini Kit from Magen (Guangzhou, China). Notably, we used mechanical disruption in liquid nitrogen instead of overnight enzymatic digestion to lyse the tissue samples in order to minimize damage to the extracted genomic DNA. Genomic DNA quality was determined by Qubit (Thermo Fisher Scientific, USA) and Agilent 2100 Bioanalyzer (Agilent, USA), and sequencing libraries were prepared by the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs, USA). Libraries were sequenced on an Illumina HiSeq X10 sequencer using 2×150 bp cycles to an average depth of 25×.