GSM3580935: CT24.2_DZnep_H3K4me3; Mus musculus; ChIP-Seq
ChIP-Seq for H3K4me3 was performed according to the Ren Lab ChIP protocol (http://bioinformatics-renlab.ucsd.edu/RenLabChipProtocolV1.pdf). ChIP-seq for BMAL1 was performed using the ChIP-IT high Sensitivity Kit (#53040, Active Motif) according to the manufacturer's instructions. Library construction was performed using the PrepX Illumina ChipSeq kit and the PrepXComplete ILMN DNA barcodes on the Apollo324 System. The resulting library was cleaned with AMPure XP beads and quantified by qPCR with Kapa Sybr Fast universal for Illumina Genome Analyzer kit. The library size was determined by analysis using the Bioanalyzer 2100 DNA High Sensitivity Chip and libraries were sequenced on the HiSeq 4000 Sequencer (100 bp, single-end reads). ChIP–seq samples were sequenced using the Illumina HiSeq 4000 .