GSM3719128: Jumonji_scRNAseq; Mus musculus; RNA-Seq
For the single cell drop-seq RNA expression profiling, GEM (Gel bead-in-EMulsions) generation & barcoding, post GEM-RT cleanup & cDNA amplification and the library construction were done according to manufacture procedures using Chromium Single Cell 3' Library & Gel Bead Kit v2 (PN-120237), Chromium Single Cell A Chip Kit (PN-1000009) and Chromium i7 Multiplex Kit (PN-120262) by 10x Genomics (Pleasanton, California, USA). For the single cell drop-seq RNA expression profiling, GEM (Gel bead-in-EMulsions) generation & barcoding, post GEM-RT cleanup & cDNA amplification and the library construction were done according to manufacture procedures using Chromium Single Cell 3' Library & Gel Bead Kit v2 (PN-120237), Chromium Single Cell A Chip Kit (PN-1000009) and Chromium i7 Multiplex Kit (PN-120262) by 10x Genomics (Pleasanton, California, USA).