GSM1560002: t1_Hi726ctr_rep2; Haemophilus influenzae; RNA-Seq
Total RNA was isolated from Hi176 infected WD-NHBE cells at 1, 6, 24 and 72 h post infection using Trizol reagent, as well as mock-infected cells and Hi176 conditioned in infection medium for 1 h. The strand-specific cDNA library was generated with ScriptSeq- Complete Gold Kit – Epidemiology, Low Input from Epicentre after ribosomal RNA depletion using Ribo-Zero Gold – Epidemiology kit