GSM5159019: DMAH105: RNA_rep2_50_min; Saccharomyces cerevisiae; RNA-Seq
Total RNA was extracted using the hot phenol method. MNase digestions were performed as previously described by Henikoff et al. 2011 and Belsky et al. 2015. RNA-seq libraries were prepared using TruSeq Stranded Total RNA-seq Kit (Illumina) and ribosomal RNA was removed with Ribo-Zero Gold Kit (Human-Mouse-Rat, Illumina). MNase-seq libraries were prepared using 2 µg of DNA as previously described by Henikoff et al. 2011, with the following modifications: NEBNext multiplex oligos for Illumina Kit (New England Biolabs) was used in adapter ligation and PCR steps. PCR reactions were performed with 12 cycles and the reactions were cleaned using Agencourt AMPure XP beads (Beckman).