GSM4704520: Thrombus scRNA-seq; Mus musculus; RNA-Seq
Thrombus and vein wall tissue were mechanically disrupted and pushed through a 40µm cell strainer. Apoptotic cells were cleared from the cell suspension via the manufacturer´s instructions (EasySep™ Dead Cell Removal -Annexin V- Kit, #17899) and CD25+ cells enriched via positive selection (EasySep™ Mouse CD25 Regulatory T Cell Positive Selection Kit, # 18782). Libraries were generated following the BD Rhapsody System mRNA WTA and Sample Tag Library Preparation protocol (BD Biosciences).