RNA-seq of breast cancer cell line
Cells were seeded in 12-well Corning cell culture plates and treated in triplicates with 20 M and 50 M deoxycholate for 24 hours. RNA extraction was performed according to the RNAeasy miniKit protocol from Qiagen. RNA integrity for each sample was confirmed with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). One g of RNA was sent to the Center for Cancer Research Sequencing Facility at the NCI for library preparation with the TruSeq PolyA kit (Illumina, San Diego, CA)