GSM4154288: KT09-Treg; Homo sapiens; RNA-Seq
RNA was extracted from biopsy samples using the Qiagen Allprep DNA/RNA mini kit. The Ovation RNA-seq System V2 protocol was implemented to amplify cDNA from 500 pg of qualified mRNA from each sample. cDNA amplification was performed using NuGEN Ribo-SPIA technology. The cDNA was then sheared as ~200 bp fragments, end-repaired, followed by A-tailing and adapter ligation reactions. The library was PCR enriched and purified using the Ampure XP bead size selection method.