GSM1567947: Dmel_ChIP-seq_wt_IP_rep1; Drosophila melanogaster; ChIP-Seq
1g of forzen embryos were fixed and chromatin was isolated and sheared to the average size of 500 bp (Omni Sonic Ruptor 250 Watt Ultrasonic Homogenizer, for 7 cycles (1 min on [Duty cycle 30%, Output 20%], 1 min off)). 500 ul of chromatin was incubated with 25 ul of anti-V5 agarose affinity gel (Sigma, A7345-1ML) for 2 hr at 4° C. 3 ng of material was used for library generation.  Library was constructed following instructions of NEBNext® DNA Library Prep Reagent Set for Illumina® (NEB; cat. no. E6000L). Library amplifcation was done using KAPA Library Amp Real Time (KAPA biosystems: cat.no. KK2701).