Salmon nasal cartilage cDNA sequencing
Poly A+ RNA was extracted from total RNA using Sera Mag Magnetic Olig(dT) microparticles (Seradyn Inc., Fremont, CA, U. S. A.), and was fragmented. Double-stranded cDNA was synthesized using reverse transcriptase and random primers, and adapters were ligaed. cDNA library was prepared following the instructions of the GS FLX Titanium rapid library preparation kit (454 Life Sciences, Roche).