GSM4104219: CD4_siNS_rep4; Homo sapiens; RNA-Seq
Total RNA was isolated from primary T cells using the Qiagen RNeasy Plus Mini Kit with on-column DNase digestion.  Total RNA integrity was assessed using automated capillary electrophoresis on a AATI Fragment Analyzer.   The integrity of all samples are RQI > 8.0 A minimum starting amount of 75 ng was used as input for library preparation using the Illumina TruSeq Stranded mRNA Library Preparation Kit