GSM2198072: 225sh_Dox_1; Homo sapiens; RNA-Seq
Total RNA was extracted using High Pure RNA isolation kit (Roche) according to the manufacturer's instructions. cDNA was obtained from 100 ng of total RNA using SuperScript VILO cDNA synthesis (Invitrogen). High RNA integrity was assessed by Bioanalyzer nano 6000 assay with RIN numbers 9.0 TruSeq®Stranded Total Sample Preparation kit (Illumina Inc.,)