GSM4705452: CD335 subset of Goat  at day 0; Capra hircus; RNA-Seq
Total RNA from each subset-CD4+, CD8+, CD14+, CD21+ and CD335+ of PBMCs of control (Goat & Sheep) and vaccinated (Goat & Sheep) were isolated using the RNeasy Mini kit (Qiagen GmbH, Germany). The integrity and quantity of isolated RNA were assessed  based on a Bioanalyzer (Agilent Technologies, Inc). The RNA integrity number (RIN) value of all the samples were found greater than 8. Library was prepared using NEBNext Ultra RNA Library Prep Kit for Illumina (NewEngland Biolabs Inc.) following the manufacturer's protocol. Approximately, 100ng of RNA from each sample was used for RNA library preparation. The quality of the libraries was assessed on Bioanalyzer and further quantified using a Qubit 2.0 Fluorometer (Life technologies). Libraries were sequenced on Illumina – NextSeq500  following manufacturer's protocol. 