Metagenome or environmental sample from Marine Bacteria and Archaea
16S V3 and V6 library preparation for the 14 samples was performed at Genotypic Technologyâs Genomics facility according to workflow outline above. 4 ng of Nanodrop quantified DNA was used for amplifying 16S Archaea region with degenerate gene-specific primers which have 5âend flanking sequence complementary to Illumina sequence adapter and index primers from the Nextera XT Index kit V2.