GSM4153728: HUES_1_WT_differentiation_replicate_2; Homo sapiens; RNA-Seq
Methods: RNA extraction for mRNA sequencing was performed post flow sorting of at least 200,000 TH+ cells using Qiagen micro RNA extraction with on-column DNase digest. RNA amount and integrity were approximated using the bioanalyzer 2100 RNA Nano kit. Illumina mRNA-seq libraries were prepared using the TruSeq RNA kit using 200 ng of total RNA per sample. Library sequencing was carried out on a HiSeq2500 in rapid mode, 1 lane/pool. Each pool contained 6 samples, 3 differentiation replicates per isogenic cell line. The concentration of both pools was approximately 2.6 ng/µl and