gyrB gene sequencing of NICU environmental swab: rm_2_Around_bed
Libraries for sequencing of the gyrB gene were prepared according to Illumina s  16S Metagenomic Sequencing Library Preparation  guide, with the exception of using Qiagen HotStar Taq DNA polymerase for the first PCR ( amplicon PCR ). The gyrB specific primers were (without the overhang adapter sequence): gyrB_aF64 (5 -MGNCCNGSNATGTAYATHGG-3 ) and gyrB_aR353 (5 -ACNCCRTGNARDCCDCCNGA-3 ). The first PCR ( amplicon PCR ) was carried out for 40 cycles with annealing temperature of 60 C. Diluted pooled samples were loaded on an Illumina MiSeq and sequenced using a 500-cycles MiSeq Reagent Kit V2.