GSM4104122: MS19270_CSF; Homo sapiens; RNA-Seq
Patient Inclusion Criterior: 1) treatment naive patients with a first episode suggestive of MS (i.e. clinically isolated syndrome (CIS)) or with relapsing-remitting (RR)MS diagnosed based on MAGNIMS criteria86, 2) patients receiving LP for diagnostic purposes and consenting to participate. Patient Exclusion Criterior: 1) questionable diagnosis of MS by clinical signs or magnetic resonance imaging (MRI) findings, 2) secondary chronic progressive MS or primary progressive MS. IIH patients were included, if they gave informed consent. Exclusion criteria for all patients were: 1) immunologically relevant co-morbidities (e.g. rheumatologic diseases), 2) severe concomitant infectious diseases (e.g. HIV, meningitis, encephalitis), 3) pregnancy or breastfeeding, 4) younger than 18 years, 5) mental illness impairing the ability to give informed consent, 6) artificial blood contamination during the lumbar puncture resulting in >200 red blood cells / μl. Chromium Single Cell Controller using the Chromium Single Cell 3' Library & Gel Bead Kit v2 AMPure beads (Beckman Coulter)  Illumina Nextseq 500 using the High-Out 75 cycle kit with a 26-8-0-57 read setup