GSM5176448: Focused_TGEV5th; Sus scrofa; OTHER
Illumina sequencing of sgRNAs in the Focused-CRISPR  cells Briefly, the genomic DNA from Focused-CRISPR  cells  were extracted using a Blood & Cell Culture DNA Midi Kit (QIAGEN), respectively. The sgRNA-coding region was amplified by PCR using Q5® Hot Start High-Fidelity DNA Polymerase (NEB). PCR products were purified with a MinElute PCR purification Kit (QIAGEN) followed by Illumina HiSeq 3000 Next-generation sequencing. Mapped read counts were subsequently used as input for the MAGeCK analysis software package (version 0.5).