Leaf, 12hour, stress
Brassica napus 3-week-old seedlings were treated with 200mM salt water. The third leaves were harvested after 12 hour. Total RNA was extracted by Promega SV Total RNA Isolation System.  mRNAs were puriﬁed using oligo(dT)-attached beads and fragmented into small pieces (100–400 bp). The cleaved RNA fragments were then primed with random hexamers and submitted to the synthesis of the ﬁrst-strand and second-strand cDNAs. The synthesized cDNAs were ligated with paired-end adaptors. Then, the cDNA fragments with 200 bp ( 20 bp) size were selected by agarose gel electrophoresis and enriched by PCR ampliﬁcation.