ONP_ACER-MSAP-product
Preselective products from 10 samples with high band representation for each selective (SL1-4) and enzyme (Hpall and Mspl) combination were pooled and amplified with non-labelled selective primers. Resulting products (n=8) were cleaned with a DNA Clean & Concentrator kit (Zymo Research, Irvine, CA), quality checked with a TapeStation D1000 ScreenTape (Agilent Technologies Inc., California, USA) on a Tapestation 4200 system and multiplexed with a Native barcoding expansion kit (EXP-NBD104, Oxford Nanopore Technologies). Libraries for Oxford NanoPore sequencing were constructed with a ligation library kit (SQK-LSK109, Oxford Nanopore Technologies, Oxford, UK) and sequenced to a total of 20GB