Modulation of gilthead sea bream gut microbiota by the addition of LSAqua SusPro
Anterior intestine mucus samples (200 l) were treated with 250 g/ml of lysozyme (Sigma) for 15 min at 37C. Then, DNA was extracted using the High Pure PCR Template Preparation Kit (Roche) following the manufacturers instructions. DNA concentration, quality and purity were measured using a Nanodrop 2000c (Thermo Scientific), and agarose gel electrophoresis (1% w/v in Tris-EDTA buffer). DNA was stored at -20C until sequencing.