GSM4154167: Mi-1; Solanum tuberosum; RNA-Seq
The libraries were prepared with the TruSeq RNA kit (Illumina, USA, San Diego, California.). The mRNA was purified from 1 μg of total RNA using oligo-dT beads, and then fragmented using divalent cations and at 95 °C. Reverse transcription was performed to obtain cDNA fragments of approximately 200 bp. After repair, adenylation and ligation of indexed adapters, the libraries were completed and amplified by PCR. The libraries were sequenced on a HiSeq 2500 instrument (Illumina, USA, San Diego, California) in Paired-end mode (2x125bp). RNA libraries were prepared for sequencing using standard Illumina protocols