GSM4932270: NG2 glia-1; Mus musculus; RNA-Seq
NG2 glia with GFP fluorescent labeling from Pdgfra-creERTM; mGFP mouse brain was sorted and suspended in calcium- and magnesium-free PBS containing 0.04% weight/volume bovine serum albumin (BSA). Then the cells were added to each channel, and the target cell will be recovered. Captured cells were lysed and the released RNA were barcoded through reverse transcription in individual GEMs. The cDNA was generated by reverse transcription and amplified. Chromium Single Cell 3ʹ Reagent Kits v3  was used  for the construction of sequencing libraries according to the manufacture's introduction.