GSM4103424: M14; Homo sapiens; RNA-Seq
Tissue samples were rinsed with sterile saline and examined under a lightbox for content confirmation and selection of molar tissue or chorionic villi. Samples were then stored in RNAlater RNA stabilization reagent (approximately 10 μL reagent per 1mg of tissue, Qiagen, Germantown, MD, USA) at 37 °C for a maximum of 24 h until collected for dissection. Samples were then dissected into ~1 g tissue aliquots and stored at -80 °C. Stored samples were then thawed at room temperature and homogenized with electric homogenizer. Total RNA was isolated using the protocol provided with the Qiagen RNeasy Mini Kit. RNA quantity was measured by a Nanodrop ND-8000 analyzer (Thermo Scientific, Waltham, MA, USA). Samples were then stored at −80 °C until used. Library preparation and paired-end sequencing were performed by the USC Epigenome Core Laboratory. Double-stranded cDNA fragments were synthesized from mRNA, ligated with adapters, and size-selected for library construction according to Illumina protocol. RNA sequencing was carried out on Illumina Genome Analyzer (Illumina, San Diego, CA, USA).