S79
Fecal DNA was isolated from using the MagAttract PowerMicrobiome DNA/RNA Kit (Qiagen) and an epMotion 5075 liquid handling system. The V4 region of the 16S rRNA gene was amplified and sequenced as previously described. Standard PCRs used 1, 2 or 7 L of undiluted DNA and touchdown PCR used 7 L of undiluted DNA.