RNA seq of Homo sapiens: non-cancer lung
Small RNAs were suspended in 1borate/borax buffer  without NaIO4 (final concentration 25mM) (Sigma-Aldrich) and incubated for 30 min at room temperature in the dark. After adding 1/10 volume of 100% glycerol, samples were further incubated for 10 min at room temperature in the dark. After purification, RNA samples were precipitated using ethanol and used as input for Solexa sequencing by Illumina 3000.