MEF_Cre_postTAM_ChIP-seq_Ctcf_rep2
1-5 ng of DNA was used for ChIP-seq library construction. ChIP DNA was end-repaired and added with dA tail using Klenow fragment. Sequencing adaptors were ligated to the dA-tailed libraries, and the libraries raging around 270 bp were selected using AMPure XP beads (Beckman Coulter). Size-selected libraries were enriched by PCR with index primers. The quantity and quality of libraries were respectively monitored by library quantification kit (Kapa Biosystems) and Bioanalyzer (Agilent) for 75bp, paired-end Illumina NextSeq.