GSM3273345: 6152_METHIONINE (bisulfite-seq); Bos taurus; Bisulfite-Seq
Total DNA was extracted from the muscle tissue for whole genome bisulfite sequencing analysis. Extraction, library construction, bisulfite treatment and sequencing were performed by Novogene Bioinformatics Technology Co., Ltd (Beijin, China). DNA was tested for purity using Nanodrop (OD260/OD280), DNA degradation and RNA contamination through an agarose gel electrophoresis and was quantified through Qubit.  DNA was fragmented into 200-300bp strands through the use of Covaris S220. Terminal repairing, A-ligation and adapters ligation were performed to the resulting fragments. The resulting DNA library was treated with bisulfite with the use of the EZ DNA Methylation Gold Kit from Zymo Research. Library concentration was quantified twice, first with Quibit 2.0 and after diluting to 1ng/ul, it was re-quantified with qPCR. 