CAVE Strain 375 genome
Genomic DNA was extracted with NZY Microbial gDNA (NZYTech, Portugal). Libraries were prepared using the Nextera XT library prep workflow (Illumina), and 2  150-nucleotide (nt) paired-end reads were generated on an Illumina MiSeq instrument. Quality trimming was executed using the sliding-window operation in TrimGalore (http://www.bioinformatics.babraham.ac.uk/projects/trim_galore/) with default parameters. The final assembly was performed using the SPAdes (10) assembler (version 3.50) using kmers of 33, 55 and 77 nt. The assembly was subjected to binning with metabat and quality check was performed on the final resulting file with checkM.