GSM1357581: s_3_16_8.0_polyA; Xenopus tropicalis; RNA-Seq
Series 1&2: total RNA was isolated using TriPure (Invitrogen) followed by LiCl precipitation. RNA was dissolved in 100 µl H2O, and 10 µg total RNA from each sample was used for PolyA+ RNA library preparation using Illumina kit RS-930-1001 Series 3: total RNA was extracted, samples were suspended in 40 µl DEPC H2O, and 1 µg was taken for library preparation. PolyA+ RNA was isolated using beads from TruSeq RNA Sample Prep Kit v2 (Illumina kit RS-122-2001), and PolyA+ RNA-seq libraries were prepared using Epicentre ScriptSeq v2. Ribosomal RNA depleted RNA-seq libraries were prepared using Epicentre ScriptSeq Complete Kit (BHMR1224) combining Ribo-Zero and ScriptSeq v2. RNA libraries were prepared for sequencing using standard Illumina protocols