GSM4395583: MDA-MB-231_3fS_unt_CUTandRUN_Ig; Homo sapiens; OTHER
Unfixed cells were immobilized on Concanavalin A-coated magnetic beads and permeablized with 0.05% digitonin. After antibody binding and pA-Mnase digestions, antibody-bound DNA fragments were released into solution, purified, and used for library construction. Sequencing libraries were constructed using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) with modification for shorter DNA fragments (< 100 bp).