Complete genome sequencing of H. alvei A23BA
DNA extraction was carried out with the DNeasy® Ultraclean® Microbial Kit for DNA isolation (Qiagen, UK). DNA was quantified with the Quant-iT dsDNA HS kit (ThermoFisher Scientific, UK) assay. Genomic DNA libraries were prepared using the Nextera XT Library Prep Kit (Illumina, USA) following the manufacturer's protocol with slight modifications. DNA quantification and library preparation were carried out on a Hamilton Microlab STAR automated liquid handling system (Hamilton Bonaduz AG, Switzerland). Pooled libraries were quantified using the Kapa Biosystems Library Quantification Kit for Illumina on a Roche light cycler 96 qPCR machine. Libraries were sequenced with the Illumina HiSeq using a 250bp paired end protocol.