GSM5076365: mPFC_FUS_NLS1_6months_old; Mus musculus; RNA-Seq
Briefly, RNA from cortex of 22-months-old male Fus∆NLS/+ mice and their control littermates were extracted with TRIzol (Invitrogen). RNA quality was measured using the Agilent Bioanalyzer system or RNA screenTape (Agilent Technologies) according to the manufacturer's recommendations. Samples were processed using the Illumina TruSeq single Stranded mRNA Sample Preparation Kit according to manufacturer's protocol. Generated cDNA libraries were sequenced using an Illumina HiSeq 4000 sequencer with 4–5 biological replicates sequenced per condition using single read, 50 cycle runs. RNA from the cortex of 6-months-old male Fus∆NLS/+ and control littermate mice was extracted and libraries were generated using the Illumina TruSeq single Stranded mRNA Sample Preparation Kit. The cDNA libraries were sequenced on a HiSeq 4000 with 3 biological replicates per condition using single-end 50bp read. Total reads sequenced varies from 35 to 45 million reads. Complete QC report will be made publicly available.