Re-sequencing of the Nipponbare genome with a 51-cycle single-end protocoll by illumina GAIIx
For the next-generation DNA sequencing, nuclear genomic DNA was prepared from Nipponabre young leaves (two weeks after germination). The DNA sample was fragmented by the protocol described in the instruction of the sample sequencing kit for the Illumina Genome Analyzer IIx, and was subsequently subjected to sample preparation and sequencing with a 51-cycle single-end protocol.