Integration sites from CD4-positive T-cells infected with HIV-1 and expressing viral proteins constitutively
Shan et al. (2011) transduced CD4  T cells with Bcl-2, costimulated with bound anti-CD3 and soluble anti-CD28 antibodies, interleukin-2 and T cell growth factor and then infected with X4-pseudotyped GFP-expressing NL4-3-d6-drEGFP construct [49] at an MOI of less than 0.1. DNA was extracted, digested with PstI and circularized (Han et al., 2004). HIV-human junctions were amplified by reverse PCR and sequenced using Sanger capillary electrophoresis. Qualities have been filled with a default 20 quality value.