GSM1846078: G2; Mus musculus; RNA-Seq
RNA was prepared with RNeasy Mini kit (Qiagen) Mouse IVT spike-in and ERCC mixes were added to the total RNA 1microgram of total RNA was processed using the Illumina TruSeq RNA Sample Preparation Kit v2 protocol and 700ng for the Illumina TruSeq Strand Specific total RNA with RiboZero Gold protocol (Illumina). Libraries were evaluated by Qubit and TapeStation. Sequencing libraries were constructed with barcodes to allow multiplexing of 9 samples on three lanes. Between 39-59 million paired-end 100-bp reads were sequenced per sample on Illumina HiSeq Rapid 2500 instrument using protocols RTA (1.17.21.3) and HCS (2.0.12.0).