Rhizoglyphus robini transcriptome sequencing - young tritonymphs
Total RNA was extracted from adult females, two male morphs, and tritonymphs. About 100 individuals in each group were used for the extraction. RNA was extracted using RNAzol-RT; residual DNA was removed by DNAse treatment. Sequencing libraries were prepared from polyA  RNA with the TrueSeq RNA kit and 2 x 100 bp paired-end (PE) sequencing was performed.