GSM3665496: ChIP-PCDH-EZH2; Homo sapiens; ChIP-Seq
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody (EZH2 and H3K27me3) or streptavidin beads (FBP1-avi). Illumina sequencing libraries were prepared from ChIP and input DNAs by performing standard consecutive enzymatic steps of end-polishing, dA-addition, and adaptor ligation (NEBNext® Ultra™ II DNA Library Prep, NEB, E7103).