WGS of apis mellifera: adult female leg
Total genomic DNA was extracted from leg tissue using a modified isopropanol protocol. Samples were lysed in Proteinase K (Qiagen) over night, followed by RNAse A (Qiagen) treatment, protein separation, and isopropanol precipitation. Extractions were quantified on a TapeStation (Agilent Genomics). Extracted DNA was prepared for sequencing with dual indexing using Tn5 transposase enzyme for tagmentation (DNA fragmentation and tagging with sequencing primers) using primers, sequencing adaptors, and barcodes suitable for the Illumina system.  The Tn5 protocol used was based on that detailed in Picelli et al.  (2014).