GSM3271868: abh1.control_I; Arabidopsis thaliana; RNA-Seq
Four rosettes per genotype from control and ozone treatment were pooled, frozen into liquid nitrogen and stored at -80°C. Three biological replicates of RNA were isolated with with GeneJet Plant RNA purification Mini Kit (Thermo Scientific). RNA quality and concentration were measured using Agilent 2100 Bioanalyzer and NanoDrop. Libraries were prepared using 1µg total RNA following instructions from Illumina TruSeq Stranded mRNA Library Prep Kit.