GSM3409494: shTLP_eto2; Homo sapiens; RNA-Seq
Total cellular RNAs were prepared from HeLa cells using RNeasy Mini Kit (Qiagen) as described by the manufacturer, and ribosomal RNA was removed from samples using RiboMinus Eukaryote Kit (Thermo Fisher Scientific) Libraries for mRNA-seq were prepared using Ion Total RNA-seq Kit v2 (Thermo Fisher Scientific). The sizes of fragmented RNA and library cDNA were determined using the MultiNA microchip electrophoresis system (Shimadzu), and the quantity of library cDNA was determined by qPCR using KAPA Library Quantification Kit (KAPA Biosystems)