liver_3
Total RNA was extracted with TRIzol reagent (Life technologies, US, Cat#74106) according to the manufacturers protocol. Integrity and concentration of total RNA were then assessed using a 2100 Bioanalyzer (Agilent Technologies, US) with the RNA 6000 Nano Kit (Agilent Technologies, US). Complementary DNA (cDNA) was synthesised and used to construct a library with the NEBNext Ultra RNA Library Prep Kit (NEB, E7530). The libraries were sequenced on the Illumina HiSeq2000 platform via 2x52-bp paired-end sequencing at BGI Tech Solutions Co., Ltd (Shenzhen, China). The RNA-Seq, libraries were sequenced at BGI Tech Solutions Co., Ltd (Shenzhen, China).