GSM3273430: 6-Shogaol; Candida albicans; RNA-Seq
To prevent RNA degradation, RNase inhibitor (RNAlater, Ambion, TX, USA) was added to cells. Total RNA was isolated using a hot acidic phenol method, and RNA was purified using a Qiagen RNeasy mini Kit (Valencia, CA, USA). After that RNA was harvested using Trizol reagent (Invitrogen). 1.3ug of total RNA was used for the construction of sequencing libraries. RNA libraries were prepared for sequencing using Clontech SMARTer Stranded RNA-Seq Kit standard protocols.