GSM1569181: mRNAseq from MEF line 129S5E36; Mus musculus; RNA-Seq
RNA from seven independent primary MEF lines was isolated (+129/+Bl6: 129S5E88, 129S5E90, 129S5E95; Df/+Bl6: 129S5E36, 129S5E56, 129S5E71, 129S5E98) using TRIzol reagent (Ambion), and polyA+ RNA was isolated (Oligotex kit; QIAGEN). Stranded libraries were prepared using a protocol adapted for paired-end sequencing on the Illumina GA IIx platform.