GSM5032517: CA28; Bos taurus; RNA-Seq
Total RNA of eight female samples per treatment was isolated from the CAR and COT tissues using the RNeasyâ kit (Qiagenâ, Germantown, MA) followed by on-column DNase treatment, according to the manufacturer's protocol. Strand-specific RNA libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, MA). Library preparation and paired-end sequencing with 150-bp reads at a depth of 20 M reads/sample were carried out at Novogene Co. (Nanjing, China).
GEO
GSM5032517