GSM2628100: met1 sRNA; Arabidopsis thaliana; ncRNA-Seq
RNA was extracted from inflorescence tissue using the TRIzol extraction method. To generate total RNA-seq libraries, RNA was subjected to ribodepletion with Ribo Zero (Illumina) and libraries were prepared with the TruSeq Stranded mRNA Library Prep Kit (Illumina). RNA 3’end libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit (Lexogen) adjusted for longer insert lengths by diluting the second strand synthesis buffer with water in a ratio 1:1. sRNA-seq libraries were constructed by Fasteris SA. Libraries were prepared using the Illumina TruSeq Kit SBS v3.
GEO
GSM2628100