GSM5032623: C3PP1: Control DE Replicate 1; Homo sapiens; RNA-Seq
RNA was extracted using TRIzol (Thermo Fisher Scientific) according to the manufacturer's protocol. The quality of extracted RNA samples was determined using a 2100 Bioanalyzer and the Agilent RNA 6000 nano kit (Agilent; Santa Clara, CA). The samples with RIN > 9 were used for mRNA library preparation. Libraries were prepared using TruSeq stranded mRNA Library Prep Kit LT (Illumina, Inc.; San Diego, CA). The concentration of the libraries was determined using Quantitative PCR KAPA Library Quantification Kit for Illumina (Kapa Biosystems), and the sequencing runs were performed on a NextSeq 500 (Illumina).
GEO
GSM5032623