GSM4989054: WT_2; Mus musculus; RNA-Seq
Left hippocampus were removed and flash frozen on dry ice. Total RNA was extracted using mirVana PARIS RNA isolation kit (Invitrogen) according to manufacturer's instructions. 500ng total RNA was used for library preparation using modifications to the Ion Total RNA-Seq kit v2 (Life Technologies) protocol. The major modifications were as follows: ligation of Ion adapters was performed at 16C for 16 hours; rRNA depletion was achieved by the use of QIASeq FastSelect rRNA removal kit (QIAGen). Samples were pooled into two mixed pools and sequenced on the Ion Proton platform on ion PI v3 chips.
GEO
GSM4989054