GSM1213138: MID2_GBM_0U; Homo sapiens; Bisulfite-Seq
Genomic DNA was extracted with phenol:chloroform:isoamylalcohol and perceipitated with ethanol and salt. Libraries were prepared for each sample using bisulfite-patch-PCR protocol. Briefly, purified genomic DNA is digested by a restriction enzyme into fragments with defined ends. After denaturation, in a multiplexed reaction, both ends of selected target loci are hybridized and ligated to specific patch oligonucleotides and universal priming sequences. After enzymatic enrichment of ligated loci, PCR is performed using primers with platform-specific adapter and universal sequences. Barcoded products are then pooled, purified and sequenced.
GEO
GSM1213138