GSM4257658: T_cell_scRNA-seq; Homo sapiens; RNA-Seq
Targeted single-cell RNA-Seq analysis was conducted using the BD Rhapsody Single-Cell AnalysisSystem (BD Biosciences,USA), following the manufacture's protocol. Briefly, double strand cDNA were firstly generated from Microbeads-captured single cell transcriptome following several steps including reverse transcription, second strand synthesis, end preparation, adapter ligation and whole transcriptome amplification. Then final cDNA library were generated from double strand full length cDNA by random priming amplification with BD Rhapsody cDNA Kit (BD Biosciences, 633773) and BD Rhapsody Targeted mRNA & AbSeq Amplification Kit (BD Biosciences, 633774). Based on BD Rhapsody system Whole transcriptome analysis alpha protocol for single cell whole transcriptome analysis, Microbeads-captured single cell transcriptome were generated into cDNA library containing Cell label and UMI information. Briefly, double strand cDNA were firstly generated from Microbeads-captured single cell transcriptome following several steps including reverse transcription, second strand synthesis, end preparation, adapter ligation and whole transcriptome amplification. Then final cDNA library were generated from double strand full length cDNA by random priming amplification with BD Rhapsody cDNA Kit (BD Biosciences, 633773) and BD Rhapsody Targeted mRNA & AbSeq Amplification Kit (BD Biosciences, 633774).
GEO
GSM4257658