GSM5252631: MTC-Q1 mir-21 KO; Mus musculus; RNA-Seq
Tumor tissues were dissociated by tumor dissociation kit (MACS) and remove dead cells by dead cell removal kit (MACS). CD45+ immune cell isolation were isolated by CD45+ microbeads. 10x Genomics Chromium Single Cell 3' Reagent Kit were used to dropped single cells and CDNA libary construction. CDNA library construction were using 10x Genomics Chromium Single Cell 3' Reagent Kit
GEO
GSM5252631