GSM4399716: Npm1c.HSPC_ATAC-seq_rep1; Mus musculus; ATAC-seq
100,000 freshly isolated cells were pelleted and resuspended in 100 µL Lysis buffer, followed by tagmentation using Nextera DNA Sample Preparation Kit (Illumina) for 30 minutes incubation at 37 °C. Transposed DNA was purified using with MinElute PCR Purification kit (Qiagen). Eluted DNA was amplified via PCR and purified with Qiagen MinElute PCR Purification kit. The quantity  and quality of library DNA was measured using KAPA Library Quantification kit (Kapa Biosystems) and Bioanalyzer 2100 system with a High Sensitivity DNA chip (Agilent Technologies) respectively.
GEO
GSM4399716