Whole exome sequece of JIGR/DN mouse strain identifies MFSD12 (gr/gr)  mutation
Five hundred nanogram of Illumina paired-end pre-capture library DNA fragments were hybridized for 16 hr at 65ĄC with Agilent SureSelect mouse exome capture probes (Agilent Technologies, Santa Clara, CA). Index the captured DNA libraries by 10 cycles of PCR amplification. Cycling conditions for the reaction were: 98ĄC for 2_min, then 10 cycles of 98ĄC for 30_s, 57ĄC for 30_s and 72ĄC for 1min, final extension at 72ĄC for 10min. The final libraries wereĘqualifiedĘand quantified using an Agilent 2200 Tapestation instrument. Equal amount of libraries were pooled for multiplexed sequencing on an Illumina HiSeqĘ2500 sequencer (Illumina, San Diego, CA, USA) using the pair-end 100bp run.
SUB2677893
JIGR/DN