GSM5343650: EN2dxA: Taenia solium egg, biological rep. 2; Taenia solium; RNA-Seq
Two cleaned Taenia solium eggs were taken up into a total volume of 4 μL of PBS on a fresh cover slide and mounted to a Leica DMiL phase contrast microscope with a HI Plan 20x lens. Two freshly prepared borosilicate needles were inserted into the drop and located under visual control next to the Taenia egg. The two needles were then moved towards each other squeezing and ultimately disrupting the egg. This procedure was repeated for the second egg. The cell lysate was immediately transferred into a 0.5 mL tube containing 0.6 μL of the optimized SMART-Seq buffer, placed on dry ice and shipped on dry ice by an international courier service to the NGS-sequencing facility (BGI, Shenzhen, Guangdong, China) RNA libraries were prepared for sequencing using standard protocols for BGISEQ-500 sequencing
GEO
GSM5343650