GSM3358796: YPR2_DD_2; Trichoderma reesei; RNA-Seq
Mycelia were harvested by filtration and frozen in liquid nitrogen. Total RNA was isolated essentially as described previously (Tisch et al., 2011 PMID  21220037) using the QIAGEN plant RNA kit (QIAGEN, Hilden, Germany) according to manufacturers instructions. Quality control of total RNA was performed using Bioanalyzer 2100 (Agilent) and only high quality RNA was used for further analysis. Next generation sequencing was performed at the core facility VetCORE (Vienna, Austria) on a HiSeq2000/SR50 machine.
GEO
GSM3358796