GSM2803861: Sham miR-143 mimic 3; Rattus norvegicus; RNA-Seq
The enucleated rat eyes were harvested and stored in the RNAlater (Qiagen), and retinas were dissected immediately under the dissection microscope. Total RNA and miRNA were isolated from the dissected retina by using a miRNeasy mini kit (Qiagen, Hilden, Germany). One microgram of miRNA samples in triplicate was prepared as per the manufacturers’ instructions (Illumina Inc. San Diego, CA, USA). The libraries of cDNA from the biological samples in triplicate were sequenced by using an Illumina Hiseq-2500 RNA-seq platform as 50 bp single-end chemistry. miRNA libraries were prepared for sequencing using a standard Illumina protocols
GEO
GSM2803861