GSM3453214: A549_4h_2.0MOI; Homo sapiens; RNA-Seq
A549 H2B-mCherry infected or MOCK infected cells were detached from the cell culture plates using trypsin-EDTA 0.05% (Gibco), washed with cell culture media and passed through a 40 mM cell strainer to prepare single-cell suspensions, and the final concentration was set at 1,000 cells/ml. Single-cell gel beads in emulsion (GEMs) were generated using the 10X Genomics Chromium system following the Single Cell 3' Reagents Kits V2 recommendations. The 10X chip was loaded to target 5,000 cells final. Reverse-transcription was performed in the emulsion and cDNA was amplified for 12 cycles before library construction. Each library was tagged with a different index for multiplexing. Quality control and quantification of the amplified cDNA were assessed by Bioanalyzer. Library quality control were evaluated by Bioanalyzer, Qubit and Kappa assays.
GEO
GSM3453214