GSM5261980: Organ of Corti_cochlear_P12_rep2; Mus musculus; RNA-Seq
Cochleae were dissected out and incubated in solution (50% accutase (Innovative Cell Technologies, San Diego), 0.02% Trypsin(Thermofisher), 125 μg/ml Thermolysin (SIGMA-ALDRICH)) at 37°C for 3 min. 0.02 mg/ml collagenase IV (SIGMA-ALDRICH) and dispase (Worthington Biochemicals Corp) were further added to the enzyme solution and incubated for 4 min at 37°C. The enzymatically treated tissue was triturated gently using fire-polished Pasteur pipettes and aggregated cells were removed using a 40-μm strainer. The dissociated cells were centrifuged at 500 × g for 5 min and resuspended in solution (0.5% fetal bovine serum, 0.04% bovine serum albumin, 0.3 mM ethylenediaminetetraacetic acid in Hanks' balanced salt solution). Library construction was performed using Chromium Single Cell 3' v2 Reagent Kits (10X genomics) following the instruction manuals. Briefly, the dissociated cells were loaded into a Chromium Controller (10X Genomics) and the encapsulated cells were lysed individually. Reverse transcription within each oil droplet was performed and the cDNAs were amplified. Single cell RNAseq  performed using an Illumina HiSeq 4000 system
GEO
GSM5261980