GSM4989348: H3K27Ac ChIP in MSC transduced with EWS-ZFA7_replicate1; Homo sapiens; ChIP-Seq
For ChIP-seq, chromatin from 1% formaldehyde-fixed cells were fragmented to 200-500 bps by sonication for 5-6 mins using the Branson sonifer SFX250 RNA-seq:Single-end libraries were prepared using the Illumina TruSeq Stranded Total RNA Library Prep Gold kit (Illumina) and TruSeq RNA Single Indexes, pooled, and sequenced using an NextSeq500. ChIP-seq: Sequencing libraries were prepared with 3 ng of each H3K27Ac ChIP DNA and input sample using SMARTer ThruPLEX DNA-seq kit (Takara, Cat# R400675). Libraries were sequenced with single-end (SE) 75 cycles on an Illumina Nextseq 500 system
GEO
GSM4989348