GSM2101029: RNA_diploid_131a; Arabidopsis thaliana; RNA-Seq
Total RNA was isolated from rosette leaves using a Plant total RNA Purification kit Cat#TR02 (Gene Mark, Taiwan). The procedure involves cell lysis and protein precipitation followed by centrifugation. RNA in the supernatant is then bound on a filter column, where it is digested with DNaseI, followed by washing, and eluting with nuclease-free waster. RNA was quantified with the Qubit RNA BR Assay and 10 μg of purified RNA was utilized as starting material for library construction, which was carried out with the Dynabeads for polyA RNA enrichment, Superscript III cDNA synthesis, and barcoded with illumina index.  Libraries were sequenced using 100-bp reads on the HiSeq 2000 System (Illumina).
GEO
GSM2101029