Effects of paternal circadian disruption on offspring transcription
Total RNA was prepared from adrenal glands using the TRIzol reagent (Thermo Fisher) according to the manufacturers instructions. RNA concentration and integrity were controlled on a Bioanalyzer system (Agilent) and only RNA samples with RIN (RNA Integrity Number) values > 7 were used for downstream applications. Sequencing libraries were prepared by using the Quantseq 3mRNA-Seq mRNA Library Prep Kit FWD for Illumina (Lexogen) with i7 indexes (Lexogen) according to manufacturers instructions. Libraries were sequenced on an Illumina HiSeq 2500 at single-end.
SUB9227695
Adrenal_gland_treated_Male_ZT6_Rep1