GSM2803215: B-14; Mus musculus; RNA-Seq
Frozen femurs were crushed in liquid nitrogen with a Bessman Tissue Pulverizer (Spectrum Laboratories, Rancho Dominguez, CA). Total RNA was isolated using TRI Reagent (Molecular Research Center, Cincinnati, OH) according to the manufacturer’s instructions. RNA was quantified using a Nano-drop spectrophotometer (Thermo Scientific, Wilmington, DE) at 260 nm. Purity was assessed using the A260/A280 ratio and quality was analyzed using the Caliper LabChip GX (Perkin Elmer, Waltham, MA). Libraries were were prepared using the Illumina TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA) Library sizes were confirmed using Caliper GX and quantified by the Kapa biosystems qPCR according to manufacturers instructions
GEO
GSM2803215