GSM3454535: KO19_4; Homo sapiens; RNA-Seq
RNA was extracted from 80% confluent cells using the RNeasy Mini Kit (Qiagen 74104) following the manufacturer's recommendations. RNA quality was assessed with the RNA Nano 6000 kit (Agilent 5067-1511) and Agilent Bionalyzer 2100 instrument. RNA-Seq libraries were prepared using the SENSE mRNA-Seq Library Prep Kit V2 (Lexogen) following the manufacturer's recommendations with 1μg of total RNA as the starting material. The size and quality of the final library products were assessed using the Agilent High Sensitivity DNA Kit (Agilent 5067-4626). Library concentration was determined using the KAPA Library Quantification Kit (KR0405). Libraries were pooled in equimolar concentrations and subjected to Illumina sequencing.
GEO
GSM3454535