GSM3454563: M_Old_8; Mus musculus; RNA-Seq
Mice were PBS-perfused, whole spinal cords were removed, flash frozen in liquid nitrogen, and RNA was harvested using Trizol reagent. Adult Brain Dissociation Kit (Miltenyi Biotec, 130-107-677) and relative protocol on gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec, 130-096-427) were used to generate single cells suspension from brain tissues. Microglia cells were identified as Cd11b+ and Cd45+ by Fluorescence-activated cell sorting (FACS) and RNA isolated with RNeasy Plus Micro Kit (Qiagen, 74034). TruSeq RNA Library Preparation Kit v2
GEO
GSM3454563