GSM5343155: Wildtype_2; Mus musculus; RNA-Seq
Lung tissue was minced and digested with collagenase IV (1mg/ml, Sigma), DNase I (0.16 mg/ml, Sigma) and dispase II (2mb/ml, Roche) for 30 minutes at 37C. The raw tissue homogenate was filtered through a 70 um filter. Next, washed cells were resuspended in 1X red blood cell lysing buffer (Abcam) for 5 minutes to remove blood cells. Cells were counted with an automated cell counter to confirm absence of cell clumps. 10X Chromium Single Cell 3' v3
GEO
GSM5343155