Oxford nanopore long reads for Draft hybrid long-read assembly of Meyerozyma guilliermondii ATCC 6260
1ug of genomic DNA were sheared in a gTube (Covaris, Woburn, MA) for 1min at 6,000 rpm in an Eppendorf MiniSpin plus microcentrifuge (Eppendorf, Hauppauge, NY). The sheared DNA was converted into a Nanopore library with the Nanopore Sequencing kit LSK-108 kit with the Expansion barcoding kit EXP-NBD103. (Oxford Nanopore, UK). The libraries were pooled in equimolar concentration and the pool was sequenced on a SpotON Flowcell MK I (R9.4) flowcell for 48hs, using a MinION MK 1B sequencer. Basecalling and demultiplexing were performed in real time with the Metrichor Agent V2.45.3 using the 1D BaseCalling plus Barcoding for FLO-MIN_106)450bp workflow.
SUB3605453
Oxford nanopore