GSM5342028: ATAC_KO-PBS [ATAC-seq]; Mus musculus; ATAC-seq
Microglia were isolated by mechanical tissue dissociation and Percoll gradient centrifugation at 4°C followed by flow cytometry of single, DAPI(-),CD11b(+),CD45(+),Ly6c(-) cells. After cell lysis, ATAC-sequencing libraries were generated according to the manufacturer's protocol of the Nextera DNA Sample Preparation Kit (Illumina, FC-121-1030). Per 80,000 cells, 50 µl tagmentation mix was added. Samples were sequenced on an Illumina HiSeq 2500 using 75 bp paired-end reads.
GEO
GSM5342028