GSM3357417: ChIPseq_Wildtype_H3K4me3_rep2; Danio rerio; ChIP-Seq
Embryos of 24 hpf were fixed, deyolked, and homogenized using pestles and sonication to isolate the chromatin In the regular non-spike in experiment zebrafish chromatin was incubated with antibody overnight. For spike-in experiments zebrafish chromatin was mixed with Drosophila spike-in chromatin (Active Motif 53083) and incubated overnight with H2Aγ (Active Motif 61686) and specific antibodies. After o/n antibody incubation the chromatin was extensive washed. The resulting ChIP-DNA was used as input for KAPA-HYPERprep library preparation.
GEO
GSM3357417