GSM3290897: 5 hpo embryos; Bactrocera dorsalis; miRNA-Seq
5, 6 and 7 post-oviposition (hpo) embryos were collected and immediately stored in RNAlater® Solution (Ambion). Total RNAs were extracted using Trizol reagent (Invitrogen, CA, USA). Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with total RNA for the construction of sequencing libraries. Small RNA libraries were generated from the three samples using the Illumina Truseq Small RNA Preparation kit according to Illumina's TruSeq Small RNA Sample Preparation Guide. The purified cDNA library was used for cluster generation on Illumina's Cluster Station and then sequenced on Illumina GAIIx following vendor's instruction for running the instrument. Raw sequencing reads were obtained using Illumina's Sequencing Control Studio software version 2.8 (SCS v2.8) following real-time sequencing image analysis and base-calling by Illumina's Real-Time Analysis version 1.8.70 (RTA v1.8.70). A proprietary pipeline script, ACGT101-miR v4.2 (LC Sciences), was used for sequencing data analysis. Degradome library was constructed following with methods previously described by German et al.(2008).CleaveLand3.0 and LC Science's ACGT301-DGEv1.0 program were used to detect potentially sliced targets of known and novel miRNA.
GEO
GSM3290897