GSM4592622: CS16 Trunk Ao; Homo sapiens; RNA-Seq
The dorsal aortae (Ao) were dissected from human embryos CS16 - sample 1 was further bisected into ventral (AoV) and dorsal (AoD) portions with only AoV taken for analysis, sample 2 was trimmed to the mid portion of the Ao. Tissues were dissociated into single cells in 1mg/ml Collagenase-Dispase. For sample 1 CD34+ cells were enriched using the human CD34 MicroBead Kit and LS MACS Columns (both Miltenyi Biotec). For sample 2, live cells were sorted using a FACS Aria-II (BD Bioscience) using 7AAD as a marker of dead cells. Libraries were prepared using the Single Cell 3' Reagent Kit v2 (10x Genomics) according to manufacturer's protocol.
GEO
GSM4592622