GSM4594095: B. subtilis PY79 (OD0.5-OD6.0); Bacillus subtilis; OTHER
Cells were fixed in cold formaldehyde overnight, permeabilized with Tween-20 and Lysozyme, then subjected to in-situ polyadenylation, reverse transcription and barcode ligation reactions. After barcoding, cells were lysed with proteinase K and barcoded cDNA was extracted on streptavidin beads Barcoded cDNA bound to streptavidin C1 beads had a 3' adapter appended through template switching, followed by qPCR amplification and fragmentation with WGS Fragmentation Mix (Enzymatics). Adapters for Illumina sequencing were then appended by the final round of PCR. SPLiT-seq, first barcode records sample identity
GEO
GSM4594095