GSM3358746: ttn1E; Xenopus laevis; RNA-Seq
Each dish was harvested at gastrula stage 10.5 in 5-embryo aliquots. Replicates were taken from two different clutches (D & E), and 5 replicates were analyzed within each clutch (10 replicates total). Each five embryo pool was homogenized in 200 μL of TriPure (Roche).  Total RNA was DNAse treated, LiCl precipitated, and 100 ng was reverse transcribed into cDNA using Ovation RNA Amplification System V2 (NuGEN). Total RNA was subjected to cDNA synthesis using the Ovation RNA Amplification System V2 kit (NuGEN). Deep sequencing libraries were generated from the cDNA using the ABI SOLiD 5500 Fragment Library Core Kit (Life Technologies).
GEO
GSM3358746