GSM4942655: HAP1_Tile18_R1DB_P2_L1; Homo sapiens; OTHER
Genomic DNA was isolated from 1-3x107 cells using the Quick-DNA Midiprep Plus kit (Zymo Research. For each sample, 16 replicate PCR reactions were assembled, each with 0.5 µg of gDNA as template (template copy number of 2.4x106 genome equivalents). Integrated inserts (~3.7 kbp), including MSH2 cDNA and associated barcode, were PCR amplified with PrimeSTAR GXL (Takara). Each set of 16 replicate amplicons was pooled and purified with SPRI beads. Tile-specific primers were used to further amplify the mutagenized tile using 10 ng full-length amplicon (≥ 2.6x109 copies) as template. Dual-indexed illumina adapters were then added by a subsequent round of PCR.
GEO
GSM4942655