RNA Seq of Leishmania infantum PP75 resistant to trivalent antimony
The resistant line (LiSbR) was previously selected in vitro by step-wise increase of SbIII drug pressure and the resistance index is 4-fold higher than of its susceptible counterpart (Liarte and Murta, 2010). These parasites were maintained in the culture under SbIII pressure and the new IC50 values for LiWTS and LiSbR were 0.12 mg/ml and 1 mg/ml, respectively, showing resistance index of 8-fold. The SbIII concentrations used in this study were obtained after detailed analysis of parasite viability. Different SbIII concentrations were used and the parasites were submitted to FACS analysis, using propidium iodide and annexin V (Immuno Chemistry Technologies). The antimony susceptible line (LiWTS) was cultivated during 24h in the absence of the drug (LiWTS 0 sample) or at a SbIII concentration of 0.06 mg/mL (LiWTS 0.06 sample) that corresponds to half of the SbIII IC50 for this LiWTS line, which does not induce apoptosis.
SUB2686334
SbR_0.06_A