RNA sequencing of a 6-day perithecial culture of Neurospora crassa (rid+)
Directional crosses were performed between strains P9-42 (Oak Ridge wild type mat a) and F2-01 (fl, mat A).  Total RNA was isolated 6-days post-fertilization using TRIzol (Life Technologies).  RNA was purified and genomic DNA was removed using a PureLink RNA Mini Kit (Life Technologies).  Ribosomal RNA was removed with a RiboZero Human/Mouse/Rat kit (Illumina, CA). The RNAseq libraries were then prepared with Illumina's TruSeq Stranded RNAseq Sample Prep kit. Pooled libraries were then quantified by qPCR and sequenced on one lane for 101 cycles from one end of the cDNA fragments on a HiSeq2000 using the TruSeq SBS sequencing kit (version 3). Fastq files were generated with Casava1.8.2.
Illinois State University
RNA sequencing of a 6-day perithecial culture of Neurospora crassa (rid )