RNA_Seq of rhizosphere bacteria: Atractylodes lancea
Total bacterial community DNA was extracted with an AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, U.S.) from the root tissues, rhizosphere soils and control soils was used for amplification and sequencing of the 16S rRNA, targeting the variable V3-V4 regions  resulting in amplicons of approximately ~ 473 bp.An Illumina Miseq platform (TruSeqTM DNA Sample Prep Kit, Illumina USA) was used to detect diversity and composition of bacterial community.
SUB8608494
NBS+B-S_3