GSM5176657: HcordbloodN1_2; Homo sapiens; ATAC-seq
20'000 induced immature cells were isolated from cultured human cord blood and processed as in Current Protocol in Molecular Biology by Buenrostro et al. in 2015 ATAC-Seq: A Method for Assaying Chromatin Accessibility Genome-Wide. Library was amplify using NEBNext High-Fidelity 2X PCR Master Mix and size selection was performed using Agencourt AMPure XP beads. The primer list can be found in Current Protocol in Molecular Biology by Buenrostro et al. in 2015 ATAC-Seq: A Method for Assaying Chromatin Accessibility Genome-Wide. sequencing protocol: Sequencing was performed by Novogene using the Illumina NovaSeq and the 150-bp paired-end configuration to obtain at least 100 million reads per sample.
GEO
GSM5176657