GSM1574336: P1_n1; Mus musculus; miRNA-Seq
Sciatic nerves were freed from perineurial fibroblast layer and homogenized with a chilled pestle. Total RNA was subsequently extracted using Qiazol according to manufacturers description. After RNA quality assessment with a Bioanalyzer 2100 (Agilent), the fraction of 15-35 nt was purified using gel electrophoresis and linked to Illumina TruSeq adapters. For multiplexing, barcodes were introduced during PCR amplification. Sequencing was performed using the Illumina HiSeq 2000 platform with a read length of 50 bp
GEO
GSM1574336