GSM2102317: p_GSI_t2_3; Mus musculus; RNA-Seq
RNA was isolated from 200-1500 cells. Samples were precipitated in 0.3M Sodium Acetate (Sigma), 2 ug Glycogen (Thermo Scientific) and 70% EtOH overnight at -20C. After washing in 70% EtOH, RNA pellets were dissolved in primer mix and incubated for 2 minutes at 70 °C. CEL-seq approach was used as earlier described in PMID: 22939981, which uses poly-T primers attached by a unique 8 nt barcode for each sample, followed by the Illumina 5' adaptor sequence and a T7 reverse transcription promotor. Samples were linear amplified and pooled into 7 libraries which were sequenced over 4 lanes on a Illumina HIseq2500. Paired-end 50 bp reads were generated, the left reads were used for barcode identification and the right reads for alignment.
GEO
GSM2102317