Transcriptome of Ranunculus cantoniensis used for comparative analyses
Total RNA was obtained using RNAisoTM Plus (Takara, Qingdao, China) and then treated with RNase-free DNase I (Takara, Qingdao, China) for 45 min according to the manufacturer’s protocols. The mRNA was isolated from total RNA using oligo (dT) magnetic beads for cDNA library construction. Double stranded cDNA was sequenced using the Illumina HiSeq™ 2000 sequencer (90 bp paired-end). Image data from the sequencer was transformed by base calling into raw sequence data, which formed the raw reads.
Wuhan Botanical Garden, Chinese Academy of Science
R_cantoniensis_2014