RNA-Seq of Ichthyosaura alpestris: adult male dorsal gland (cloaca)
One wild-caught male Ichthyosaura alpestris was euthanised by spraying 0.2-0.5 ml of a 10% lidocaine solution on the head followed by decapitation and pithing of the brain. Pheromone-producing glands were excised from the body cavity and stored in RNALater solution (Sigma-Aldrich). Total RNA was extracted from the pheromone-producing glands using TRIreagent (Sigma-Aldrich) and the RNeasy Mini Kit (Qiagen). Whole transcriptome shotgun sequencing (RNA-Sequencing, paired-end, 48 Mio 50 bp read pairs) was performed at Baseclear (Leiden, The Netherlands), using an Illumina TruSeq RNA sample preparation kit for sequencing on the Illumina HiSeq 2500 platform (Illumina, San Diego, California). The raw data from Illumina HiSeq were deposited in the NCBI Short Read Archive (SRA) database.
Vrije Universiteit Brussel (VUB)
IchAlp_AEL002