GSM4257451: NoIPTGinductionRep2; Escherichia coli; OTHER
The reporter plasmid was isolated from IPTG induced and uninduced cultures and DNA was amplified from this region with oligos containing the necessary Illumina adapters. The five nucleotide PAM library was inserted inot the target plasmid backbone using Q5 mutagenesis.
GEO
GSM4257451