GSM2101453: Stele RNA-seq; Arabidopsis thaliana; RNA-Seq
Fluorescent Activated Cell Sorting (FACS) was performed using cell specific GFP lines. Sorted cells were collected directly into specific lysis buffers that were compatible with downstream applications. Cells used for bisulfite sequencing, mRNA-seq, smRNA-seq were lysed in Buffer AP1 (Qiagen), Buffer RLT (Qiagen), Trizol (Invitrogen). All samples were immediately stored at -80 °C until gDNA and RNA was extracted using DNeasy Plant mini kit (Qiagen) and RNeasy Plant mini kit (Qiagen) or Trizol, respectively. RNA-seq library preparation was performed using the Illumina TruSeq RNA Library Prep kit from polyA+ selected mRNA as per manufacturer’s instructions.
GEO
GSM2101453