GSM1207878: MCF7 profiling library 1; Homo sapiens; RNA-Seq
Eluted protein-RNA complexes were RNase I treated, followed by ammonium sulfate precipitation. Precipitate was separated by SDS-PAGE and transferred onto a nitrocellulose membrane. RNA was extracted from membrane by proteinase K treatment and phenol/chloroform extraction. Recovered RNA was dephosphorylated using CIP. After dephosphorylation RNA was phenol/chloroform extracted, ethanol precipitated and 50 end labeled with T4 PNK. Radiolabeled RNA was extracted and subsequent small RNA cloning and adaptor ligations were performed as described previously in small RNA cloning protocol (Hafner et al. 2008 Methods).
GEO
GSM1207878