GSM2974490: siNT_2; Homo sapiens; RNA-Seq
RNA was isolated from cells using the GENEzol TriRNA Pure Kit (GeneAid). RNA ScreenTape kit (catalog #5067-5576; Agilent Technologies, Santa Clara, CA), D1000 ScreenTape kit (catalog #5067-5582; Agilent Technologies), Qubit® RNA HS Assay kit (catalog # Q32852; Invitrogen, Carlsbad, CA) and Qubit® DNA HS Assay kit (catalog #32854; Invitrogen) were used for each specific step for quality control and quantity determination of RNA and library preparation. For mRNA library preparation: TruSeq RNA Library Prep Kit v2 was used (Illumina). In brief, 1 g was used for the library construction; library was eluted in 20 l of elution buffer. Libraries were adjusted to 10 mM, then 10 l (50%) from each sample was collected and pooled in one tube. Multiplex sample pool (1.5 pM including PhiX 1.5%) was loaded in NextSeq 500/550 High Output v2 kit (75 cycles cartridge and 150 cycles cartridge; Illumina, San Diego, CA). Run conditions were in paired end (43X43bp and 80X80 bp, respectively) and loaded on NextSeq 500 System machine (Illumina).
GEO
GSM2974490