GSM4593992: SPL_B_CELLS_1; Mus musculus; RNA-Seq
Total RNA was isolated using the PureLink RNA Mini Kit (Thermo-Fisher Catalog # 12183018A) using the "on column DNA digestion" protocol variation (PureLink DNase, Thermo-Fisher Catalog # 12185010).  RNA quality was assessed via bioanalyzer (Agilent) and quantified via NanoDrop (Thermo).  The indicated amount of total RNA ("characteristics:  total RNA input") was used for the construction of sequencing libraries. RNA sequencing libraries were constructed with TruSeq Stranded mRNA Library Prep from Illumina (Catalog # 20020594) using the manufactuer's standard protocol.  Libraries were indexed with TruSeq RNA Single Indexes Set A (Catalog # 20020492).  Library quality was assessed via bioanalyzer (Agilent), and quantified via Qbit (Thermo).  Libraries were normalized and pooled for sequencing (splenic dendritic cells and monocyte-derived macrophages were pooled and run separately).
GEO
GSM4593992