GSM1215619: sham; Danio rerio; RNA-Seq
total RNA was extracted using QIAGEN RNeasy Microarray Tissue Mini Kit (Cat No. 73304) Poly (A)-positive RNA was purified with the Dynabeads mRNA purification kit (Invitrogen) from 5μg of total RNA with high quality (RNA integrity number >7.5), following the manufacturer's instructions. A strand-specific RNA-Seq library preparation on the basis of the incorporation of deoxy-UTP was performed; Exome fragments were captured and enriched by SureSelect Human All Exon 50 Mb kits (Agilent Technologies) following the manufacture’s protocol.
GEO
GSM1215619