Illumina HiSeq 4000 sequencing; Transcription profiling of developing xylem of Populus maximowiczii × P. trichocarpa and P. nigra × P. maximowiczii growing pure and mixed with Robinia at poor and rich soil sites
Transcription profiling of developing xylem of Populus maximowiczii × P. trichocarpa and P. nigra × P. maximowiczii growing pure and mixed with Robinia at poor and rich soil sites
Bark was peeled of stems, developing xylem tissue was scraped off with razor blades and was directly frozen in liquid nitrogen. Poplars were planted with stem cuttings at two field sites: Deppoldshausen (51.581°N/9.967°E) and Reinshof (51.484°N/9.923°E), Germany. Planting took place during April 2014. After growing for 3 years under ambient weather conditions, plants were harvested in 2017. Treatment conditions:a) The two field sites Deppoldshausen and Reinshof differ in soil nutrient levels.b) Both poplar species were planted in blocks, either as monoculture or in mixture with Robinia. Nucleic acid was extracted according to CTAB extraction protocol (Chang et al., 1993). RNA libraries were prepared using the TrueSeq RNA Library Prep Kit (Illumina, San Diego, CA, USA) according to manufacturer's protocol.
Georg-August-Universitaet Buesgen-Institut Department of Forest Botany and Tree Physiology
E-MTAB-9028:Sample 6_s