Human Lung Cancer Cell line
Cells (HCC827 or NCI-H322) were cultured in triplicate dishes, and after harvesting of cells using trypsination, total RNA was extracted using RNeasy kit (Qiagen, Hilden, Germany) according to manufactures instructions. RNA libraries were created using strand-specific TruSeq kit with poly-A selection for all RNA samples according to manufactures instructions. Quality control were checked by Bioanalyzer/Caliper. Sequencing (Paired-end 2 x 125 bp) was performed by HiSeq2500 (Illumina, San Diego, CA, USA) as standard RNA-seq protocol.
SUB4308725
Lung Cancer Cell lines NCI-H322_CtrlA