GSM2602197: Female_MEF_WT_HiC_Biological_Rep1_Technical_Rep2; Mus musculus; Hi-C
Hi-C libraries were generated with the in-situ ligation protocol using the HindIII restriction enzyme.  Briefly, ~25 million cells were crosslinked with 1% formaldehyde for 10 minutes at room temperature.  Then, the chromatin was digested with HindIII, end-labelled with biotin-14-dCTP, and in-situ ligation was performed.  Following phenol-chloroform extraction, the biotin was removed from unligated ends, and the DNA was sheared by using a Covaris S220 instrument. After A-tailing, biotin pull-down and adapter ligation, paired-end sequencing was performed on a HiSeq instrument.  Each Hi-C library was generated in at least two biological replicates from MEFs prepared from at least two distinct mouse embryos.
GEO
GSM2602197_2