GSM3724624: DNA-PKcs PQR/PQR -1; Mus musculus; OTHER
Genomic DNA was extracted from day 4 cultured B cells with standard DNA extraction protocol. Basically cells were washed with PBS before lysed overnight with rapid lysis buffer containing 10mM tris PH 7.5, 10mM EDTA PH8.0, 10mM NaCl, 0.5% SDS and 1mg/ml Proteinase K at 56 °C. DNA was precipated from lysed cells with isopropanol and washed with 70% EtOH before air dry and re-dissolving by 10mM tris/ 0.1mM EDTA TE buffer. Libraries were prepared according to protocol in (Hu J, et al. Nat Protoc 11:853–871.(2016)) Linear amplifications were performed using Sμ-specific biotin primer  5′/5BiosG/CAGACCTGGGAATGTATGGT3′) and followed by nesting PCR with (5′CACACAAAGACTCTGGACCTC3′)  AflII is used to remove germ-line sequence. high-throughput genome translocation sequencing (HTGTS). Sequenced with MiSeq 500v
GEO
GSM3724624