Illumina HiSeq 2000 sequencing; Salt stress in salt sensitive Oryza sativa Indica group IR29 (salt sensitive)
Salt stress in salt sensitive Oryza sativa Indica group IR29 (salt sensitive)
Oryza sativa L. Indica IR29 seedlings were grown in growth chambers to the 2-3 leaf stage. At 2 weeks old, they were subjected to salt stress treatment by watering with 300 mM NaCl solution (or normal watering for control plants). Treatment was applied at 10:00 PST (2 hours post-dawn). Seedling shoots (stem and leaves) were sampled at 1 hour, 2 hours, 5 hours, 10 hours, and 24 hours post-treatment. Total cellular RNA was extracted using RNA Plant Reagent (Life Technologies) and treated with RNase-free Turbo DNase (Life Technologies) followed by a clean-up step using Plant RNeasy kits (Qiagen). Concentration, integrity and extent of contamination by ribosomal RNA were assessed using ND-1000 spectrophotometer (Thermo Fisher Scientific) and Bioanalyzer 2100 (Agilent Technologies). For details see Fox et al. (2009) Applications for Ultra-high-Throughput Sequencing. In: Belostotsky (ed.), Plant Systems Biology, vol. 553, pp. 79--108. Libraries were prepared using the TruSeq(TM) RNA Sample Preparation Kits (v2).
EMBL-EBI
E-MTAB-1913:ir29_control_5hrs_rep3 assay