Illumina HiSeq 4000 paired end sequencing; BS-seq of Arabidopsis pol2a mutants, of related mutants combinations and of WT and pol2a plants treated with hydroxyurea
BS-seq of Arabidopsis pol2a mutants, of related mutants combinations and of WT and pol2a plants treated with hydroxyurea
13-day-old rosette were cut out and flash frozen in liquid nitrogen Plants were grown in soil in a growth cabinet at 23°C, 50% humidity, using long day conditions (16h light, 8h dark). Genomic DNA was extracted using the Wizard® Genomic DNA Purification Kit (Promega) following manufacturer's instructions.  One microgram of DNA was used for bisulfite treatment and library preparation at the Beijing Genomics Institute (Shenzhen, China).
GReD - CNRS UMR 6293 - Inserm U1103
E-MTAB-8940_2:pol2a-12 2mM hydroxyurea_p