GSM5176986: NC ctrl 72h 6; Mus musculus; RNA-Seq
Differentiating brite adipocytes were washed with PBS and scraped into Trisure (Bioline). Total RNA was prepared from Trisure (Bioline) and column-purified (SV Total RNA Isolation System, Promega). Libraries were prepared from total RNA by tagmentation of full-length ds cDNA (5 ng) with Tn5 enzyme (1 µl, 11 µM) and amplified (15 cycles). AMPure beads (Beckman Coulter, #A63881) were used to size-select fragments of 200-1000 bp in two rounds (0.5x beads and 0.7x beads, respectively). Libraries were profiled with the High Sensitivity NGS Fragment Analysis Kit (Advanced Analytical, #DNF-474), measured with the Qubit dsDNA HS Assay Kit (Invitrogen, #Q32851), pooled and sequenced on the Illumina NextSeq 500 platform with custom primer and High Output v2 kit (75 cycles) (Illumina, #FC-404-2005).
GEO
GSM5176986