RNA-seq of Bacillus valezensis FZB42
After treatment bacterial were centrifuged and pellets immediately frozen in liquid nitrogen. Pellets were then resuspended in 500l lysis buffer (30mM Tris, 10mM EDTA, 10mg/mL lysozyme) for 30 min in 37 . RNA was prepared using the RNAzol reagent according to the manufacturers instructions. rRNA was removed using NEBNext rRNA Depletion Kit (Bacteria) according to the manufacturers instructions. RNA-seq libraries were prepared using KAPA RNA HyperPrep Kit (Roche) according to the manufacturers instructions.
SUB9398267
WT_IAA_3