Comparative transcriptomics analysis between maize and its progenitor
Total RNA was extracted from whole plant using a standard TRIzol protocol, purified with a MinElute PCR Purification Kit, library was eluted in 10 µL of Nulease-free water. The eluted cDNAs were then enriched by 18 cycles of PCR, followed by gel purification. Library adapters used balanced barcode sequences of six base pairs. Pools of 16 (including 10 maize inbredlines and 6 teosinte collections) libraries were sequenced on Illumina sequencing platform.
Huazhong Agricultural University
YE478