HDAC3FFvsdIEC Longitudinal Study
The bacterial 16S rRNA gene was amplified from stool DNA samples and pyrosequenced for comparative analysis at 6, 8, and 10 weeks of age.
Each amplicon library was constructed by amplifying the16S rRNA gene using the BSF8 and and the BSR357 primer pair. Primers contained DNA barcode sequences such as those described by Hamady et al. 2008, and the recommended 454 adapter sequences. Amplification conditions are described in McKenna et al 2007, with exception that the polymerase used was AccuPrime (Invitrogen, Carlsbad, CA, USA).
UPENNBL
H4RRC5N_region1