ITS-Seq: forest soil
The total community DNA was extracted from soils by use of a MoBio Power Soil DNA extraction kit. The ITS gene was amplified in triplicate using the barcoded ITS1F and 2043R primer set. PCR products were quantified, purified and sequenced by Illumina Miseq platform for assessment of fungal diversity and community structure.
SUB1609495
WF_3_fungi