Seasonal dynamics of the honey bee gut microbiota in colonies under subtropical climate
To further identify the changes in the composition and abundance of different genera belonging to the gut microbiota, DNA from 3 colonies at 6 sampling points (October and December 2014; February, April, June and August 2015) was analyzed through high throughput sequencing. The V4 region of 16S rRNA gene was amplified using primers Hyb515F_rRNA and Hyb806R_rRNA ([27], Supplementary data, Table 1) and sequenced using an Illumina MiSeq platform and 250 paired end (PE) cycles at the Genomic Sequencing and Analysis Facility, University of Texas at Austin, USA.
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