GSM2803335: Mouse2_scRNAseq; Mus musculus; RNA-Seq
The tunic was removed from the tesetes using forceps. Individual seminiferous tubules were then isolated and cut using McPherson-Vannas microscissors. The tissue was enzymatically disrupted using collagenase, hyaluronidase, dispase, and DNaseI for 30 minutes at 37°C. After passing the cells through a 40 µm cell strainer, barcoded cDNAs for each cell were generated using the Chromium 3' single-cell platform with the v2 chemistry. Sequencing libraries were constructed according to the protocol provided with the 10X Chromium 3' v2 chemistry. scRNA-seq (Chromium platform)
GEO
GSM2803335