GSM4718887: Tet-TKD T33; Mus musculus; Bisulfite-Seq
GVOs were isolated 48h after PMS injection and injected with either control morpholino or morpholinos targeting Tet1-3. After in vitro maturation and in vitro fertilization, fertilized 2-cell embryos were collected 29hpf and subjected to BS-seq library preparation. For library preparation a Post-Bisulfite Adapter Tagging approach (PBAT) (Lee et. al, PMID: 29224070) was used with few changes. Changes: BD buffer (Qiagen) was used for the desulphonation reaction, biotin-capture was omitted and oligos were changed to Oligo 1: TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGTNNNNNNNNN and oligo 2: GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGTNNNNNNNNN, to match the primers from the Nextera XT Index Kit, which were used for the library amplification in the last step using 12 cycles.
GEO
GSM4718887