S.aureus D592 RPMI+10%LB 0.5ug/mL, 3hr timepoint
Zymo Research Fungal/Bacterial RNA prep kit was used to isolate and purify the RNA. The cells were lysed using a Roche MagnaLyzer instrument. RNA quality was checked with an Agilent Bioanalyzer instrument and ribosomal RNA was removed using an Illumina Ribo-Zero kit. The final sample enriched for mRNA was used in the first step of the Kapa Biosystems RNAseq library prep kit.
SUB5305271
S.aureus D592 RPMI+10%LB 0.5ug/mL Nafcillin Rep 2a