GSM3666126: MeCP2_KO_BS_rep3; Mus musculus; Bisulfite-Seq
Genomic DNA was extracted from 8-week-old MeCP2 KO and WT forebrain with the Qiagen DNeasy kit and sonicated to 200 bp with a Covaris M220. OxBS-seq libraries were generated as described in (Stroud et al., 2017). DNA was ligated to NEXTflex methylated adapters (Bioo Scientific). Bisulfite conversions and oxidation reactions were performed using the TrueMethyl oxBS-seq kit following the manufacturer instructions (Cambridge Epigenetix). Half of the sample was used for BS-seq without oxidation treatment, and half the sample was used for oxBS-seq as recommended by the manufacturer.
GEO
GSM3666126