GSM1574536: CAT2_RNA; Homo sapiens; RNA-Seq
DNA extraction for PVU-Seq: DNeasy Blood and tissue kit (Qiagen, Valencia, CA) was used to extract genomic DNA from the fresh frozen tissues and MDA-MB-468 cells. RNaseA treatment was done according to the manufacturer’s instructions [4µL of RNaseA (100mg/mL)] to obtain RNA free genomic DNA. Extraction of the RNA was done using the RNeasy plus kit (Qiagen, Valencia, CA) according to manufacturer's instructions ThruPLEX-FD prep kit (Rubicon Genomics, Ann Arbor, MI) was used to prepare DNA libraries for PVU-Seq. Illumina TruSeq (Low Sample) RNA library preparation was used according to the manufacturer's instructions
GEO
GSM1574536