GSM1213988: MMCD; Solanum lycopersicum; RNA-Seq
Total RNA was isolated using TRIzol reagent (Invitrogen) according to the manufacturer’s protocol and DNA was removed with DNase I (Takara, JAPAN) treatment. small RNA library: After total RNA isolation, low molecular weight RNAs (LMW) were isolated as described Hafner et al., 2008.By polyacrylamide gel electrophoresis, the sRNAs were purified and ligated to 5’ and 3’ RNA adapter. A reverse transcription reaction followed by low cycle PCR was performed to obtain sufficient product for Solexa sequencing. PCR products were collected by gel purification and analysis based on Solexa high-throughput sequencing takes the SBS-sequencing by synthesis. degradome library: The tomato degradome library was constructed as previously described German et al., 2009. In this study, we utilizing identical copy of samples which proposed for sRNA deep sequencing to identify degradome by parallel analysis of RNA ends (PARE) in high-throughput sequencing. The degradome data of MMC and TY1C were marked as MMCD and TY1CD, respectively; in addition, we balanced mix DNA of 21dpi and 30dpi samples of MMS or TY1S, and marked MMCD and TY1CD, respectively.
GEO
GSM1213988