GSM3898356: RNAseq_wt_2; Mus musculus; RNA-Seq
Hippocampi were micro-dissected after cervical dislocation, washed in ice-cold PBS and pooled together, when fixation was needed 1% FA was used. Nuclei were isolated using our improved FANS protocol for SUN1-GFP+ cells. Extensive description of protocols indicated in Supplemental Experimental Procedures. Extraction of RNA was performed using TRI-reagent (MERCK) as previously described (Scandaglia et al., 2017). Libraries were prepared with TruSeq.
GEO
GSM3898356