Amplicon seq, adenine base editing, SGCA c.157G>A mutation, on-target, human muscle stem cells
PCR amplicon SGCA locus. Primers: fwd(5>3) TCTTTGTGCACACCTTGGAC / rev(5>3) GAGGACTCAGATACCAAATTAGAGG. Q5 DNA Polymerase. Amplicon checked with 2100 Bioanalyzer using DNA 1000 Kit (Agilent).
SUB9312277
SGCAex2-ABE-NGS-3_2020-05-28