Genome sequencing of Aspergillus arachidicola CBS 117610
The genome was sequenced using an Ion Torrent Personal Genome Machine (PGM) from LifeTech- nologies (Grand Island, New York). To obtain genomic DNA, 75 ml of potato dextrose broth was inoculated with 500 μl of fungal spore suspension and placed in an orbital shaker set at 30 °C and 125 rpm overnight. Resulting myce- lial pellets were then vacuum-filtered, with two sterile water washes, blot dried, and approximately 200 mg was used to extract DNA using a Masterpure Yeast DNA Purification kit (Epicentre Biotechnologies, Madison, Wisconsin). The template was first subjected to fragmentation using the Ion Xpress Plus Fragment Library kit. Template purification steps were performed using AgenCourt AmPure XP beads (Beckman Coulter, Inc., Indianapolis, Indiana) and a mag- netic rack, followed by re-suspension with Low TE buffer. Size selection of DNA fragments was performed using the E-Gel system (Life Technologies) and library qualification was performed with an Agilent 2100 Bioanalyzer instru- ment and High Sensitivity DNA kit (Agilent Technologies, Santa Clara, California). A OneTouch 2 (OT2) instrument and OT2 400 kit (Life Technologies) were used to prepare template-positive ion sphere particles (ISPs). The ISPs were qualified using a Qubit fluorometer and then enriched using the Ion OneTouch ES system with its respective com- ponent of the OT2 400 kit (Life Technologies). Sequencing was performed using the Ion PGM Sequencing 400 kit, an Ion 318 Chip v2, and the PGM instrument (Life Technolo- gies) following manufacturer’s instructions.
SUB2675204
A_arachidicola