GSM3454915: WT/C57 Replicate 2 Inferior Olive 12 Weeks Old; Mus musculus; RNA-Seq
Cerebella and inferior olive were dissected out, flash frozen, and kept at -80°C until further processing. For inferior olive, the brainstem was removed and the inferior olive dissected using the decussation of the pyramid and pons as a reference. Anatomical location was verified under a dissection microscope. The same individual conducted all tissue extractions to eliminate variability in sample collection. RNA was extracted using a Qiagen RNeasy Mini Kit, following the manufacturer's protocol. RNA was sent to the Yale Center for Genome Analysis for library prepration and sequencing. The RNA libraries for all experiments were conducted using standard Illumina protocols.
GEO
GSM3454915