GSM1215871: BNST/POA Day 60 Male 3; Mus musculus domesticus; Bisulfite-Seq
Genomic DNA was isolated from the BNST/POA using Qiagen AllPrep DNA/RNA Mini Kit (catalog no. 80204). Genomic DNA was digested with MspI. They were then ligated with Illumina adaptors, and size selected. Fragments from 100 to 200 bases were selected. These fragments were denatured and treated with sodium bisulfite. Libraries were sequenced using the Solexa sequencing technology (Illumna Hiseq 2000 sequencers). The reads were aligned to the reference genome (mouse mm9) using the modified bisulfite aligner, BS Seeker.
GEO
GSM1215871