Illumina HiSeq 2500 paired end sequencing; Raw reads: A1_Denervated_RNA-seq
Total RNA from control and denervated muscle samples from four animals was extracted as described in Section 4.2. RNA quality was analyzed with Agilent 2100 Bio- analyzer (Agilent Technologies, Santa Clara, CA, USA) using the RNA Nano Kit 6000 (Agilent technologies). For library preparation, we only selected control and denervated samples from the same animal with an RNA Integrity Number (RIN) ~10. Library con- struction and data analysis were performed by the Center for Genomic Regulation (CRG, Barcelona, Spain). RNA-seq libraries were constructed with TruSeqv4 mRNA Sample Preparation Kit (Illumina, San Diego, CA, USA) and sequenced (50-base paired-end) in a HiSeq 2500 platform (Illumina).
IdIBGi
webin-reads-A1_Denervated_RNA-seq