GSM3666783: WT1_LV; Mus musculus; RNA-Seq
Total RNA was isolated from left ventricular tissue of Nppb KO mutants and wildtype littermates using Tri Reagent (Sigma Aldrich) according to the manufacturer's protocol (Sigma Aldrich) and further purified using Nucleospin RNA Clean-up Kit with DnaseI treatment (Macherey-Nagel) cDNA was prepared with the Truseq Stranded Total RNA Library Preparation Kit (Illumina, Part# 15031048 Rev. E). Total RNA (900 ng) was purified and sheared into small fragments followed by cDNA synthesis and ligation of the adapter. The quality and size distribution of the cDNA library templates were validated with Agilent DNA 1000 on 2100 Bioanalyzer (Agilent Technologies). cDNA samples were pooled per lane of paired-end 125 bp sequencing on an Illumina HiSeq 2500 instrument.
GEO
GSM3666783