GSM4399947: PFC_CS_#8; Mus musculus; miRNA-Seq
Two hours after the (day 10) CPP test, the animals were killed and their mPFCs surgically excised. The tissue was stored in liquid nitrogen immediately after extraction and then transferred to the -80°C freezer. Total RNA was extracted from the frozen tissues using the TIANamp DNA/RNA Isolation Kit (TIANGEN), including additional treatment with RNase-free DNase I (Ambion) for 30 minutes at 37°C to remove contaminating DNA. 18-30nt RNA segments were separated and recovered by PAGE gel. The steps which followed were the same as for generating the mRNA sequencing library from the addition of poly-A. The miRNA DNBs were loaded into the patterned nanoarrays and single-end reads of 50 bp were read through on the BGISEQ-500 platform at Beijing Genomics Institute (BGI; Shenzhen, China).
GEO
GSM4399947