GSM2974228: planktonic state biological rep1; Flavobacterium columnare; RNA-Seq
Bacterial cells were harvested and stored in RNAprotect Bacteria Reagent (Qiagen) at -80°C until usage. Total RNA was extracted from each sample by enzymatic lysis and proteinase K digestion using the RNeasy Mini Kit (Qiagen) according to the supplied protocol. DNA was removed from total RNA using Amplification-grade DNase I from Invitrogen.Total RNA was standardized to 5 µg each sample and then ribosomal RNAs (rRNA) were reduced using the Ribo-Zero Gold rRNA Removal Kit (bacteria) from Illumina. Sequencing libraries were then sent to a service provider (Novogene, Chula Vista, CA) for library preparation, where the provider created strand-specific libraries via the NEBNext Ultra Directional RNA Library Prep Kit from New England Biolabs. NEBNext Multiplex Oligos for Illumina were used as index primers.
GEO
GSM2974228