GSM5031933: N_2: Naïve rep2 RNA-seq; Mus musculus; RNA-Seq
Spleens were recovered, crushed through a nylon mesh and treated with Gey's solution red blood lysis buffer. The naïve CD4 T cells were purfied as CD44lo CD62Lhi RNA was extracted using the RNeasy Plus Micro Kit (Qiagen) Libraries were constructed using the Smart-SeqTM v4 UltraTM Low Input RNA Kit for Sequencing (Clontech) and the Nextera XT library prep kit and the Nextera XT index kit (Illumina)
GEO
GSM5031933