GSM3665962: PROseq_MEF_preC_12HS_BR2; Mus musculus; OTHER
After treatments, the cells were harvested by centrifugation, washed with PBS, and permeabilized. Run-on reactions were performed with biotinylated nucleotides as described by Kwak H, Fuda NJ, Core LJ, Lis JT. Precise maps of RNA polymerase reveal how promoters direct initiation and pausing. Science 2013 Feb 22;339(6122):950-3. The run-on reaction was conducted in the presence ofpermeabilized S2 cells, which provides an invariant whole-genome spike-in control between samples Libraries were generated using TruSeq small-RNA adaptors, universal molecular indexing (UMI) barcodes (where applicable) and the 5' adaptor sequences are indicated for each individual raw data.
GEO
GSM3665962