GSM5456815: KL3_leaf_AV_24h; Brassica napus; RNA-Seq
RNA was extracted from the ground plant tissue by use of the ISOLATE II RNA plant kit according to the manual, including a DNAse treatment (Bioline, Luckenwalde, Germany). RNA was further processed by Eurofins Genomics Europe Shared Services GmbH (Ebersberg, Germany). Integrity and quantity of the RNA was determined by appropriate methods, i.e., measurement of volume and quantity, gel electrophoresis and fluorimeter measurements. Library preparation incorporated adaptor sequences and indexing compatible for Illumina sequencing technology, using proprietary methods of Eurofins Genomics Europe Shared Services GmbH. The cDNA library preparation was performed following optimized protocols. After first strand synthesis, the second strand synthesis was performed using dUPT. The ends of the double stranded cDNA fragments were repaired and dATP ligated to the blunt ended fragments. Next, the sequencing adapters were ligated to the DNA fragments, and the dUTP containing second strand was removed. Determination of size distribution and quantification of the sequencing library was accomplished with appropriate methods according to optimized protocols. Sequencing was performed on the Illumina HiSeq 4000 platform.
GEO
GSM5456815