Poly-(A) RNA was purified from 2 ľg of total RNA using the Dynabeads mRNA DIRECT Micro Purification Kit (Thermo Fisher Scientific, USA), according to manufacturer's instructions. Strand-specific whole transcriptome libraries were prepared using the Ion Total RNA-Seq Kit v2.0 (Thermo Fisher Scientific, USA). Barcoded primers were used to amplify the libraries with the Platinum PCR High Fidelity polymerase (Thermo Fisher Scientific, USA). Size distribution analysis and quantification of the final barcoded libraries was performed on D1000 Screen Tapes on the Tape Station 2200 (Agilent Technologies, USA).
SUB1607496
Monos6h_Ctrl_vitD_Repl2