GSM5170683: KN99 - WT day 3, replicate 1; Cryptococcus neoformans; RNA-Seq
Cells were lyophilized and then treated with Trizol and agitated with silica beads in an Omni bead mill homogenizer. RNA was extracted from whole cell extract by phase separation with 1 volume chloroform to produce a phenol:chloroform ratio of 1:1, and then washed with 1 volume chloroform. RNA was precipitated in 50% isopropanol, then recovered by centrifugation for 30 min at 18400xg at 4 C and washed with 70% ethanol. RNA from whole cell extract was polyA selected with Streptavidin MagneSphere® Paramagnetic Particles (Promega) and DNase treated with RNA Clean & Concentrator Kit 5 (Zymo). RNA-seq libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina and mixed before being run over multiple lanes
GEO
GSM5170683