Amplicon sequence of T cell:male DRESS patient at age 47
To separate the CD41/CD81 T-cell fractions from patients sample, PBMCs were isolated by density-gradient centrifugation (Percoll, GE Healthcare, Little Chalfont, UK) according to standard protocols.total RNA was extracted and reverse transcribed into cDNA,A set of forward primers, eachspecifictooneorasetoffunctionalTCRV-betasegments,anduniversalreverse primersspecifictotheconstantregionofTCRB,wereusedtogenerateampliconsthat covered the entire CDR3 region.
SUB7027797
WanDJ03CD8