GSM2802531: wt_B; Arabidopsis thaliana; RNA-Seq
Total RNA was extracted from A. thalinana tissues using TRIzol Reagent (Ambion) following the manufacturer’s instructions, and was treated with RQ1 DNase (Promega) to remove DNA. For polyA capture libraries, polyadenylated mRNAs were purified and concentrated with oligo(dT)-conjugated magnetic beads (invitrogen) before used for directional RNA-seq library preparation. For rRNA depletion libraries, mRNAs were purified using Ribo-zero rRNA removal kit-plant (Illumina). Purified mRNAs were iron fragmented at 95℃ followed by end repair and 5' adaptor ligation. Then reverse transcription was performed with RT primer harboring 3' adaptor sequence and randomized hexamer. The cDNAs were purified and amplified and PCR products corresponding to 200-500 bps were purified, quantified and stored at -80℃ until used for sequencing.
GEO
GSM2802531