GSM5342015: KO_PBS_641 [mRNA-seq]; Mus musculus; RNA-Seq
Microglia were isolated by mechanical tissue dissociation and Percoll gradient centrifugation at 4°C followed by flow cytometry of single, DAPI(-),CD11b(+),CD45(+),Ly6c(-) cells. RNA was isolated using a Qiagen RNeasy Micro Kit (Qiagen, 74004) according to the manufacturer's instructions. After RNA isolation, sequencing libraries were generated using NEXTflex® Rapid Illumina Directional RNA-Seq Library Prep Kit (BiooScientific, NOVA-5138-10) with polyA selection. Pooled libraries of the training model were sequenced using the NextSeq 500/550 High Output v2 kit (75 cycles, Illumina, FC-404-2005) with single reads on a NextSeq500 (Illumina). Pooled libraries of the tolerance model were sequenced with a HiSeq Rapid SBS kit (50 cycles, Illumina, FC-402-4022) using single reads on a HiSeq 2500 (Illumina).
GEO
GSM5342015