GSM5032003: 19_Mabs_B9_BE; Mycobacteroides abscessus ATCC 19977; RNA-Seq
In five independent experiments, colonies of M. absecssus were resuspended in sterile 1x PBS and diluted in Sauton's minimal media to an OD of 0.05 and grown in a 75cm2 cap-vented culture flask, in a 37 degrees C static incubator for 5 (T1) or 7 (t2) days. After harvesting at 5 or 7 days, cells pellets were spun down and immediately frozen at -80 degrees C until RNA isolation . Cell pellets in TRIzol were transferred to a tube containing Lysing Matrix B, and vigorously shaken at max speed for 30 s in a FastPrep 120 homogenizer three times. This mixture was centrifuged at max speed for 1 min and the supernatant was transferred to a fresh tube. RNA was isolated using phenol-chloroform extraction method, followed by DNase treatment. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO
GSM5032003