DNA-seq of D. melanogaster: fatbody temperature sensitive system Luciferase RNAi 2 day treatment
Genomic DNA was sonicated to an average of 300bp (QSonica Q700). One microgram of sonicated DNA was used for the library preparation (Illumina TruSeq DNA PCR-Free LT kit, FC-121-3002), according to the protocol. Libraries were quantified using the KAPA Library Quantification Kit (Kappa Biosystems KK4854). Single-end sequencing was performed with the Illumina NextSeq 500.
SUB3095843
FbtsLuci_2D_Lane4_Rep1