GSM5177038: BM169; Homo sapiens; RNA-Seq
To prepare single cell suspensions biopsies were minced and digested using Collagenase II (Worthington Biochemical Corporation) and DNase (Sigma) and processed through the gentleMACS Dissociator (Miltenyl) utilizing a nasal mucosa protocol for processing36. Following tissue dissociation, cells were passed through a 70μm filter (Falcon, Corning), washed, and counted with a Cellometer Auto 2000 (Nexcelom). Single-cell suspensions were loaded onto a 10X Chromium Controller (10X Genomics) and library preparation was performed according to the manufacturer's instructions for the 10X Chromium Next GEM Single Cell Library kit v3 (10X Genomics).  Libraries were then pooled in groups of 4 and sequenced on 4 lanes on a NextSeq500 sequencer (Illumina) using 10X Genomics recommended reads configuration.
GEO
GSM5177038