GSM4402981: HepG2_Fr-1_Seq; Homo sapiens; OTHER
Cell extracts for the chromatin fractionation were prepared as follows: treatment with formaldehyde, solubilization, and fragmentation by sonication. Total RNA was prepared using an RNeasy MinElute Cleanup Kit (#74204, Qiagen). The DNA in the fractions was sheared with an LE220 Focused-ultrasonicator (Covaris), and converted to a sequence library using a KAPA Hyper Library Preparation Kit (KAPA Biosystems). Total RNA from HepG2 cells was converted to a sequence library using a KAPA Stranded mRNA-seq Kit (KAPA Biosystems).
GEO
GSM4402981