GSM5032159: 504_121R; Homo sapiens; RNA-Seq
Tumor pieces or cells were lyzed and proteins were subjected to pull down with a GW182 peptide bound to anti-FLAG M2 magnetic beads and RNA at a size of 19-35 nt was gel extracted and used to generate library The quality and quantity of the RNA samples was checked using an Agilent bio-analyzer. Small RNA-SEQ libraries were generated using Illumina small RNA SEQ kits using the Illumina provided protocol containing library fragments 140-160 bp in size. RNA-Seq; sequenced on an Illumina HiSEQ4000 using Illumina provided reagents and protocols
GEO
GSM5032159