GSM2628239: healthy peripheral 2; Homo sapiens; RNA-Seq
Total RNA was extracted from CD141+DC synovial fluid samples and healthy control peripheral blood and the quality of all RNA samples was evaluated using an Agilent Bioanalyzer. RNA samples were reverse transcribed and sequencing libraries constructed using NuGen Ovation Universal RNA-Seq System (CAT# 0402-A01) according to the manufacturer’s protocol.  The resulting sequencing libraries were analyzed using the Caliper LabChip GX and quantified using KAPA qPCR.  Libraries were then normalized and pooled in one batch of 6 and one batch of 5.  Each pool was clustered and sequenced on an Illumina NextSeq500 instrument using 2x100 bp paired-end reads, following the manufacturer’s protocols.
GEO
GSM2628239