iCLIP of drosophila Mi-2 replicate 1
Individual nucleotide-resolution UV-crosslinking and immunoprecipitation 2 was performed as described in (Buchbender et al., 2020). Briefly, S2 cells expressing GFP-tagged dMi-2 (Lenz et al., 2021) and untagged S2 cells were washed with cold 1X PBS. Protein-RNA cross-linking was performed at 254 nm UV light (300 mJ/cm2) using a Stratagene Stratalinker.
SUB9442353
S2_Mi2_iCLIP_exp_rep1