PacBio RSII sequencing of phage L cI-40 13-am43
SMRTbell library was constructed from a genomic DNA sample sheared to an average size of ~10 kb using the G-tubes protocol. Single-strands were removed, ends repaired, and fragments ligated to hairpin adapters. Incompletely formed SMRTbell templates were eliminated by digestion with a combination of Exonuclease III and Exonuclease VII (New England BioLabs; Ipswich, MA, USA). Sequencing employed the PacBioRS2 instrument using the DNA/Polymerase Binding Kit P4, MagBead Loading Kit, and Sequencing Kit 2.0 (all Pacific Biosciences).
SUB7825488
PacBio L cI-40 13-am43