GSM4718636: MRC5_24_R2; Homo sapiens; RNA-Seq
Total RNA was extracted at 0, 6, 24, 48, 72 and 96 hours post-HCoV-229E infection, using NucliSENS® easyMag®, according to the manufacturer's protocol. At each time point, three biological repeats were tested. All RNA samples were treated with RQ1 RNase-free DNaseI (Promega, USA), according to the manufacturer's protocol. RNA quality was assessed using the Agilent High Sensitivity RNA ScreenTape for TapeStation System (Agilent Technologies, Germany). RNA samples (>8 RNA integrity number (RIN)) were prepared using the Illumina TruSeq RNA Library Preparation Kit v2 and sequenced in Illumina HiSeq 2500 Sequencer.
GEO
GSM4718636