GSM3636578: Foxp3-GFP_WT_Rep3; Mus musculus; RNA-Seq
Single-cell suspensions were purified by total CD4+ T cell enrichment followed by FACS-sorting stored in 40 µl RNAlater™ Stabilization Solution at -80 °C Samples were processed using the QIAshredder Kit (Qiagen) according to the manufacturer's protocol RNA was extracted from samples using the RNeasy Plus Mini Kit (Qiagen) according to the manufacturer's protocol RNA Libraries were prepared using the SmartSeq2 protocol on an automated Hamilton NGS-STAR library preparation system and sequenced using a HiSeq 2500 System (Illumina)
GEO
GSM3636578