GSM4815252: DRC2: L4 DRG dorsal root crush; Mus musculus; RNA-Seq
Whole DRG's were dissociated with enzyme mix (Papain, Collagenase) to a single cell suspension, washed in 1X PBS+0.2%BSA solution, passed through a 40-micron cell strainer and FACS sorted using live/dead staining. Sorted cells were loaded on the 10X Chromium system. single-cell RNA-Seq libraries were prepared using GemCode Single-Cell 3′ Gel Bead and Library Kit (10x Genomics). polyA selection based RNA capture as per the manufacturer's instructions provided by 10X genomics platform.  Libraries were prepared as per manufacturer's insturctions provided by 10X genomics platform (https://www.10xgenomics.com/single-cell/). libraries were then constructed using standard instrutions of the 10x plaform.
GEO
GSM4815252