GSM5343702: siRING1AB-proseq-rep-2; Homo sapiens; OTHER
For PRO-seq, total nascent RNA with biotin labeled was isolated from human cells. For PRO-seq, two μg of total RNA was used to start RNA-seq library construction. Nascent RNA was isolated and further reverse transcribed to cDNA. The resulting cDNA was ligated with adapters, amplified by PCR, and further cleaned to obtain the final library. Libraries were sequenced on an Illumina Hiseq machine to obtain 150 bp paired-end reads.
GEO
GSM5343702