GSM1571857: Emb_MO-Ct_24hpf_rep1; Danio rerio; miRNA-Seq
For each library 1 μg of total RNA was submitted to the small RNA v1.5 sample preparation protocol (llumina Inc., San Diego, USA) cDNA was amplified with 13 PCR cycle. cDNAs with size between 90 to 100 nt were purified independently for each sample by 10% Novex TBE polyacrylamide gel electrophoresis (Invitrogen) and eluted into 300 µl elution buffer (Illumina) for at least 2 hours at room temperature, to enrich for molecules containing inserts in the range of 18–33 nt.
GEO
GSM1571857