GSM5456590: F2: Female adult Rep 2; Mus musculus; RNA-Seq
Bone endothelial cells were purified using endomucin expression  and sorted directly into lysis buffer of the RNeasy Plus Micro Kit (QIAGEN). Total RNA was isolated as per the manufacturer's guidelines. RNA quality was checked using a 2100 BioAnalyzer (Agilent), and libraries were prepared with NEBNext® Ultra™ II Directional RNA Library Prep Kit (Illumina) following the manufacturer's guidelines. Sequencing of libraries was carried out at the LMS Genomics Facility on an Illumina Nextseq500 platform with 40-nucleotide-length paired-end reads.
GEO
GSM5456590