GSM3455686: ATAC-seq_Bcat_Cerebellum_GFPpos_R2; Mus musculus; ATAC-seq
For ATAC-seq, ~50,000 GFP-positive FACS-sorted cells were resuspended in ice-cold lysis buffer (0.25 M sucrose, 25 mM KCl, 5 mM MgCl2, 20 mM Tricine-KOH, 0.1% Igepal CA-630) and immediately centrifuged at 500 x g for 10 minutes at 4° Celsius to prepare nuclei.  The resulting nuclear pellet was resuspended in a 50 ul reaction volume in Tn5 transposase and transposase reaction buffer (FC-121-1030, Illumina Inc., San Diego, CA) and the tagmentation reaction was continued for 30 minutes at 37° Celsius. Tagmented DNA was purified using QIAGEN MinElute GelExtraction kit (28604, Qiagen). ATAC-seq libraries were PCR amplified for 11 cycles. Agencourt AMPure XP beads (A63880, Beckman Coulter) were used to purify ATAC-seq libraries, which were then paired-end sequenced for 36 cycles on a NextSeq500.
GEO
GSM3455686