GSM3898565: naïve_FM-_rep1; Mus musculus; RNA-Seq
Lungs were removed, single suspension cells were achieved by enzymatic digestion of tissue, and alveolar macrophage subsets were harvested by FACS. RNA was harvested using Qiagen RNA micro plus kit. Clontech Ultra Low Input RNA SMARTer mRNA amplification kit was used for the initial RNA amplification. The obtained dscDNA is then converted into a sequencing library using Kapa Hyper Prep Kit. Sequenced on Illumina HiSeq 4000 with 50bp paired end reads
GEO
GSM3898565