GSM3358775: cpr1 aba1_Rep1; Arabidopsis thaliana; ncRNA-Seq
Total RNAs were isolated from 15-day-old seedlings with TRI reagent (Molecular Research Center, TR118). Total RNA extracted from 15-day-old seedlings of both wild type and mutants. For library construction, 30μg total RNA were resolved on a 15% polyacrylamide gel with 8M urea. Small RNAs in the 15-40 nt size range were recovered as described (Liu et al., 2012). Small RNA libraries were prepared using the library preparation kit (NEB, E7100), and sequenced with an Illumina HiSeq2500 platform at BerryGenomics, China.
GEO
GSM3358775