GSM5170722: iPSC derived WT macrophages interferon-a+ 4-octyl itaconate; Homo sapiens; RNA-Seq
Cells were stained with propidium iodide and dead cells and doublets were removed by FACS. Live cells (including those undergoing apoptosis) were used to make single cell suspensions using the 10x Genomics platform. The single cell suspension was loaded onto a well on a 10x Chromium Single Cell instrument (10x Genomics). Barcoding and cDNA synthesis were performed according to the manufacturer's instructions. Briefly, the 10x™ GemCode™ Technology partitions thousands of cells into nanoliter-scale Gel Bead-In-EMulsions (GEMs), where all the cDNA generated from an individual cell share a common 10x Barcode. The  cDNA  libraries  were  constructed  using  the  10x  ChromiumTM  Single  cell  3'  Library  Kit  according  to  the  manufacturer's  original  protocol.   Libraries were sequenced on an Illumina NovaSeq 6000 2x50 paired-end kits using the following read length
GEO
GSM5170722