GSM3415050: ChIP-seq of 5M-GS cells using anti-H3K27me3 antibody replicate 1; Mus musculus; ChIP-Seq
Cells were cross-linked with 1% formaldehyde for 10 min. Soluble cell lysates were prepared by sonication or both MNase treatment and sonication. Lysates were incubated with protein A or protein G Dynabeads conjugated with anti-H3K4me3 or anti-H3K27me3 antibodies, and bound fractions were eluted following manufacturer's recommendation. After reversal of cross-linking for elutes and input samples, DNA from input and immunopreciptation fractions was sheared to an average size of approximately 150 bp by ultrasonication, and used for constructing ChIP-seq library.
GEO
GSM3415050