GSM2802578: old15h_rep2_Ribo-seq; Saccharomyces cerevisiae; OTHER
For ribosome footprinting, the clarified lysate from the same number of young and old cells were digested with RNase I and separated through a 10.5-ml 10%–50% sucrose gradient. 80S monosome fractions were collected and the RNA was extracted following the same procedure as for global footprints. The TruSeq Ribo Profile kit RPYSC12116 (formerly ARTseq) was used to sequence ribosome-protected mRNA fragments. The Ambion ERCC RNA spike-in control (#4456740, Life Technology) was sonicated to reach an average size of 28nt and included in the library construction.
GEO
GSM2802578