GSM2976004: Grans_I10; Mus musculus; RNA-Seq
B lymphocytes (B220+ FSC-Alow), erythroblasts (Ter119+ CD44+, FSC-Amid/high), granulocytes (Mac1+ Gr1+) and high-end progenitor/stem (Lin- Kit+ Sca1+) were sorted from the bone marrow of a C57BL/6 10-13weeks old female. T cells (CD3+ FSC-Alow) were isolated from the thymus of the same mouse. Bone marrow and thymus were dissociated mechanically, washed and stained with antibodies for 1hr on ice. Single cells were deposited on a 384 well plate using an Aria cell sorter (Beckman). Index data was collected. Single cells were indexed sorted into 384 well plate format directly into lysis buffer as adapted from Celseq2 protocol CelSeq2 (adapted at WEHI), barcode sequences are in bases 1 to 6 in read 1 and UMI sequences are in bases 7 to 14 in read 1.
GEO
GSM2976004