GSM2629855: WT 2 (FAIRE-Seq); Mus musculus; FAIRE-seq
The fixed dentate gyri from  were sonicated and then preceded to phenol chloroform extraction and the top layer was transferred to a new tube. TE buffer was then added to the old tube, followed by one more phenol chloroform extraction. The top layer was transferred and combined with the saved top layer, the combined top layers were then followed by two more rounds of phenol chloroform extraction. The purified DNA is FAIRE-seq DNA. The FAIRE-seq libraries were constructed using Nugen Ovation ultralow system V2 Kit according to its manual. Single 50bp sequencing was performed, using an Illumina HiSeq2000 sequencer.
GEO
GSM2629855