RNA-Seq of anaerobic sludge:medical stone sludge bulk
DNA Extraction and Barcoded Amplicon Sequencing were carried out.The variable region V3-V4 of the 16 s RNA gen were amplified for constructing the bacterial community DNA using the primer pairs, i.e., 341F/806R for bacteria (5-CCTAYGGGRBGCASCAG-3)/(5-GGACTACHVGGGTWTCTAAT-3). The archaeal primer pairs were Arch1106F/Arch1378R for archaea (5-TTWAGTCAGGCAACGAGC-3)/(5-TGTGCAAGGAGCAGGGAC-3), which spanned the V8 variable regionof the 16 s RNA gen.
SUB7102726
MO2-Archaebacteria