silk gland transcriptome of Bombyx mori
The collected samples were homogenized in a Micro Smash disrupter (Tomy, Japan). Total RNA was extracted using a Total RNA Kit II (Omega Bio-tek, USA). RNA quality and quantity were measured on an Agilent Bioanalyzer 2100 system (Agilent Technologies, USA). Transcript library construction and sequencing were performed at BGI-Tech, Beijing. In brief, 3 g RNA per sample was used for mRNA-seq library construction. After total RNA was digested with DNase I, mRNA was enriched using oligo(dT)-decorated magnetic beads. The mRNA was fragmented into short fragments, which were then transcribed into first- and second-strand cDNAs. Following purification, end reparation, single nucleotide A (adenine) addition, adapter ligation, and suitable fragment selection, the libraries were generated by PCR. The Agilent 2100 Bioanalyzer was used for quality control during sample library assessment. Finally, the libraries were sequenced on Illumina HiSeqTM 2000 instrument.
SUB5461633
MSG-A