GSM2100503: E14Tg2A_2DIV_Rpl10a-IP_rep2; Mus musculus; OTHER
Precipitated ribosomes were washed 4 times in 1mL high-salt buffer (20 mM HEPES/KOH pH 7.3; 350 mM KCl; 10 mM MgCl2; 1% IGEPAL; 0.5 mM DTT; 100 μg/mL Cycloheximide), and RNA was extracted using the RNEasy Micro kit (Qiagen) as per supplier’s protocol. The results were quantified by spectrophotometry (Nanodrop) and capillary electrophoresis (Bioanalyzer RNA Nano kit) 2 μg of RNA were ribosome-depleted using the RIBO-Zero Gold kit (Illumina); sequencing libraries were prepared using the SCRIPTSeq v2 kit (Illumina) according to manufacturer's instructions.
GEO
GSM2100503