Illumina HiSeq 4000 paired end sequencing; RNASeq of human mesenchymal stem cells (6 donors) with and without treatment with 1 hour of cyclic tensile strain (2.6 – 6.2% strain, 5.0 Hz).
RNASeq of human mesenchymal stem cells (6 donors) with and without treatment with 1 hour of cyclic tensile strain (2.6 – 6.2% strain, 5.0 Hz).
Human mesenchymal stem cells were treated with and without cyclic tensile strain (2.6 – 6.2% strain, 5.0 Hz, 1 hour) and incubated at 37°C, 5% CO2 for either 0 or 24 hrs. Following incubation, cells were washed with PBS, dissociated using trypsin EDTA, pelleted and frozen at -80°C for future use.  RNA was extracted from cell pellets using the RNeasy Mini kit (Qiagen) and an on-column DNA digest performed using the RNase-Free DNase kit (Qiagen). The RNA was eluted in water and the quality and quantity assessed using a NanoDrop ND-1000 spectrometer (Thermo Fisher).  TruSeq® Stranded mRNA assay (Illumina, Inc.) according to the manufacturer's protocol
UNIVERSITY OF MANCHESTER
E-MTAB-7925:Sample 2_p