Illumina MiSeq paired end sequencing
V3 16S rRNA using primers 341F and 518R
DNA extracts were prepared for paired-end MiSeq sequencing (Illumina, CA, USA) using a two-step PCR method to amplify the V3 hypervariable region of bacterial and archaeal 16S rRNA genes, and to attach MiSeq adapter and barcode sequences.
UWASH
ena-EXPERIMENT-UWASH-31-12-2019-11:43:06:839-114
University of Washington
ena-EXPERIMENT-UWASH-31-12-2019-11:43:06:839-114