GSM3667150: TGA4_mChx_Rep1; Arabidopsis thaliana; RNA-Seq
For protoplast samples, approximately 10,000-20,000 cells were FACS sorted into RLT buffer and frozen. For whole tissue, roots were transferred into microfuge tubes and flash frozen in liquid nitrogen, pulverized with a bead beater and RLT buffer was added. Total RNA was extracted using the RNeasy Micro Kit (Qiagen) with on-column DNase treatment. mRNA was purified using Dynabeads Oligo (dT). Libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina. Final quantity was determined using the DNA TapeStation and KAPA Library Quant qPCR.
GEO
GSM3667150