GSM5261667: IFNg_Ctrl_rep1; Mus musculus; RNA-Seq
Cells were collected with qiazol (Qiagen) and stored at -80°C until further processing. RNA was extracted with the miRNeasy mini kit (Qiagen) according to manufacturer's instructions. Contaminating DNA was degraded by treatment of the samples with RNase-free DNase (Qiagen) in column. 0.1-1ug of RNA was used to make RNA-seq libraries with the TruSeq Stranded Total RNA Library Prep Gold kit (Illumina) according to manufacturer's instructions. Three replicates per condition were performed with primary OPCs obtained from different litters. Libraries were sequenced on an Illumina Novaseq 6000 with a 150-8-8-150 read set-up.
GEO
GSM5261667