GSM4592558: Osrdr6-mei smallRNA-seq rep-3; Oryza sativa Indica Group; miRNA-Seq
Wild-type and Osrdr6-mei anthers at the leptotene-like stage, determined according to floret length were collected and frozen in liquid nitrogen. The plant materials (wild type and Osrdr6-mei) used for the assays were obtained from the offspring of Osrdr6-mei/+. Anthers with wild-type genotype of OsRDR6 were used as the control group, while anthers with Osrdr6-mei genotype were used as the mutant group. Each sample contains fresh anthers from 3 independent plants. And both of these groups were set up three sets of biological repeats. In this condition, the repeatability of data is good. The same sample collection method is used for transcriptome sequencing and  WGBS. Small RNA library construction and sequencing were completed by Oebiotech corporation, Shanghai, China (http://www.oebiotech.com/)   Total RNA samples used for small RNA sequencing were also used for transcriptional sequencing. Library construction and sequencing were completed by Oebiotech corporation, Shanghai, China (http://www.oebiotech.com/).  Wild-type and Osrdr6-mei anthers at the leptotene-like stage (as the same stage as used for small RNA sequencing), determined according to floret length were collected and frozen in liquid nitrogen. Library construction and sequencing were completed by Oebiotech corporation, Shanghai, China (http://www.oebiotech.com/).
GEO
GSM4592558