RNA-Seq of Ambystoma mexicanum: adult male cloacal glands
One captive-bred male Ambystoma mexicanum was euthanised by spraying 0.2-0.5 ml of a 10% lidocaine solution on the head followed by decapitation and pithing of the brain. Cloacal glands were excised from the cloacal region and stored in RNALater solution (Sigma-Aldrich). Total RNA was extracted from the pheromone-producing glands using TRIreagent (Sigma-Aldrich) and the RNeasy Mini Kit (Qiagen). Whole transcriptome shotgun sequencing (RNA-Sequencing, paired-end, 64 Mio 100 bp read pairs) was performed at DNAVision (Gosselies, Belgium), using an Illumina TruSeq RNA sample preparation kit for sequencing on the Illumina NextSeq 500 platform (Illumina, San Diego, California). The raw data from Illumina NextSeq were deposited in the NCBI Short Read Archive (SRA) database.
Vrije Universiteit Brussel (VUB)
AmbMex_AEL004