Gene expression analysis applied to a human model of PMM2-CDG
Cell cultures were synchronized at passage 5 by serum starvation to reduce bias in gene expression. Total RNA was extracted using the RNeasy Micro Kit (QIAGEN). Purified RNA samples were sequenced at the National Centre for Cardiovascular Research (CNIC, Madrid, Spain) using an Illumina HiSeq 2500 platform. The kit used for library preparations was the New England BioLabs Next Ultra RNA Library Prep Kit
SUB10027142
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