GSM5176506: WT; Mus musculus; RNA-Seq
Atherosclerotic aorta was digested with 1 mg/ml Collagenase  A for 7 min (Roche, Cat 11088785103) at 37 °C to remove the adventitia under microscope. Aortic tissue  was cut into small pieces and subjected to enzymatic digestion with 1.5 mg/ml Collagenase A and 0.5  mg/ml Elastase (Worthington, Cat LS006365) for 40 min at 37 °C while shaking. Viable CD45+  cells were sorted by FACS Aria III (BD Biosciences) with gating on live, single cells using ive/dead viability dye eFluor 450  (Thermo Fisher Scientific). Cells were captured on a 10x Chromium instrument (10x Genomics, Pleasanton, CA) using the V2 chemistry for 3' whole transcriptome analysis. Manufacturer's instructions for GEM (Gelbead EMulsion) generation, downstream reverse transcription, cDNA isolation, PCR amplification and library preparation were followed without any significant alterations
GEO
GSM5176506