GSM2801185: H23_H3K27ac_REP2; Homo sapiens; ChIP-Seq
Cells were cross-linked with 1% formaldehyde for 10 min at RT. The crosslink reaction was stopped by addition of Glycin at the final concentration of 0.2M. H23 Chromatin extract was prepared as follows: the cell pellets were dissolved and incubated in the ice-cold 0.1% SDS lysis buffer (50 mM Hepes KOH pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% Sodium Deoxycholate, 0.1% SDS, protease inhibitors) for 10 min on a rotation at 4°C. Following centrifugation, the cells were incubated in the ice-cold second lysis buffer (10 mM Tris-CL pH 8.0, 200 mM NaCl, 1 mM EDTA pH. 8.0, 0.5 mM EGTA pH 8.0, protease inhibitors) as described above. The cells were then dissolved in 0.5% SDS sonication buffer (3.106 cells per 135mg sonication buffer), directed to chromatin fragmentation by Covaris for 10 min ChIP-seq library for sequencing was prepared as the manufacture's protocol: Mondrian™   SP+ Library Preparation (NuGen)
GEO
GSM2801185