GSM1215094: CSB input DNA; Homo sapiens; ChIP-Seq
Cells were collected in Buffer B (150 mM NaCl, 0.5 mM MgCl2, 20 mM HEPES pH7.8, 10% Glycerol, 0.5% Triton X-100) and soluble CSB was removed from nuclei by centrifugation at 1500 RPM for 5 min at 4°C.  The resulting cell pellets were subsequently fixed with 1% formaldehyde for 10 min at room temperature. Crossed-linked cells were sonicated at 40% amplitude (30 sec on, 90sec off, for 24 min total) using The Branson 101-135-126 Sonifier.  Chromatin IP (ChIP) was performed using monoclonal anti-CSB antibody against the N-terminal 507 amino acids (1B1), To generate the library from ChIP experiments, ChIP-Seq Library Protocol with multiplexing was used. This protocol was developed before Illumina released its multiplex truSeq ChIP-seq kit, and the details can be found on http://ngsc.med.upenn.edu/. According to the protocol, 10 ng of ChIP DNA product was used to start the library construction.
GEO
GSM1215094