GSM2801033: mC_pYES2_LINE3_B; Saccharomyces cerevisiae; Bisulfite-Seq
Phenol-chloroform extraction Genomic DNA was fragmented with a Covaris S2 sonicator to a mean length of 200 bp, then end-repaired, A-tailed, ligated to methylated Illumina TruSeq adapters, and subjected to 4 cycles of PCR amplification with KAPA HiFi Uracil+ DNA polymerase (KAPA Biosystems).
GEO
GSM2801033