GSM1576137: Control-seedling; Zea mays; RNA-Seq
Total RNA was extracted using Trizol reagent (Invitrogen) and was subjected to the Illumina RNA-seq protocol, followed by sequencing using the HiSeq2000 platform. Seedling and young leaf tissues harvested from plant materials with and without Dp3a were used for RNA-seq analysis. Extracted nuclei were digested with micrococcal nuclease (Sigma-Al drich) for ChIP experiments using the maize CENH3 and ph-H2A-T133 antibody.The ChIP-enriched DNA of Dp3a was treated with sodium bisulfite and then performed Illumina sequence. The maize CENH3 is a rabbit polyclonal antiserum and was raised against the peptides: C-RPGTVALREIRKYQKS; STSATPERAAGTGGR-C. The ph-H2A-T133 antibody was generated with a single phosphorylated Thr at maize H2A (AAB04687.1) position 133 (LPKK(pT)AEKA). Keyhole limpet hemocyanin (KLH)-conjugated peptide was used to immunize rabbits.
GEO
GSM1576137