GSM5077861: CD11+ myeloid cells from tumor-bearing cerebellum treated with GDC-0449, PM44; Mus musculus; RNA-Seq
Mononuclear cells were extracted from mouse cerebellum, brain, tumor tissue using Percoll discontinuous gradient separation and subpopulations were isolatd using either CD11+ or CD45+ microbeads Next-generation sequencing libraries were prepared using the 10x Genomics Chromium Single Cell 3' Reagent kit v2  per manufacturer's instructions. Libraries were uniquely indexed using the Chromium i7 Sample Index Kit, pooled,  and sequenced on an Illumina Hiseq sequencer in a paired-end, single indexing run. Sequencing for each library  targeted 20,000 mean reads per cell. scRNA-seq
GEO
GSM5077861