GSM1210207: H3 ChiP-seq spt6 37º; Saccharomyces cerevisiae; ChIP-Seq
IPs from 2 mg of cross-linked extract were immunoprecipitated, and prepared for illumina library construction by repair with Klenow, T4 DNA polymerase and T4 polynucleotide kinase, A-tailing with Klenow exo-, ligation to branched adaptors, gel purification of ~200bp fragments, and amplification (18cycles) with Illumina PE primers using Phusion DNA polymerase.
GEO
GSM1210207