Illumina MiSeq paired end sequencing
Construction of a mixed viral metagenomic dataset using 25 DNA and RNA viruses
The DNA library was prepared using the PureLink viral RNA/DNA kit (Life Technologies - Invitrogen) but with substitution of the carrier RNA with linear acrylamide (Life Technologies - Invitrogen). Following  treatment with RNase A (Epicentre),  the REPLI-g single cell kit (Qiagen) was used for amplification of DNA. Libraries were generated using Nextera XT library preparation kit (Illumina).
PUBLIC HEALTH ENGLAND
ena-EXPERIMENT-PUBLIC HEALTH ENGLAND-04-08-2015-13:17:24:219-2