GSM4990510: TS576-1A3; Homo sapiens; RNA-Seq
Total RNA was extracted from GBM-spheres using RNeasy Plus Mini Kit (Qiagen # 74134). 2000 ng of total RNA dissolved in RNase-free ddH2O was shipped for sequencing to Novogene Corporation Inc. After the QC procedures, mRNA from eukaryotic organisms is enriched using oligo(dT) beads. First, the mRNA is fragmented randomly by adding fragmentation buffer, then the cDNA is synthesized by using mRNA template and random hexamers primer, after which a custom second-strand synthesis buffer (Illumina) , dNTPs, RNase H and DNA polymerase I are added to initiate the second-strand synthesis. Second, after a series of terminal repair, A ligation and sequencing adaptor ligation, the double-stranded cDNA library is completed through size selection and PCR enrichment. The qualified libraries are fed into HiSeq/MiSeq sequencers after pooling according to its effective concentration and expected data volume.
GEO
GSM4990510