GSM5222724: OXM_Week-8_iPSC-macrophages; Homo sapiens; RNA-Seq
Lysates were passed through QIAshredder columns (QIAGEN) and the total RNA extracted using a QIAGEN RNeasy Mini kit, according to the manufacturer's protocol using on-column DNase treatment (QIAGEN). RNA samples were eluted in 50 µL of RNase-free water. The quantity and quality of RNA was measured by Nanodrop and RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies), with measured RNA integrity (RIN) values ≥8.8.The mRNA fraction was selected from the total RNA for each sample before conversion to cDNA. Sequencing libraries were generated using NEBNext® Ultra TM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations
GEO
GSM5222724