GSM5177035: DivisionRecorder+ memory OT-I T cells; Mus musculus; RNA-Seq
Spleens were collected from 3 mice, homogenized to a single cell suspension, treated with NH4Cl to lyse erythrocytes, and DivisionRecorder+ OT-I T cells (CD8+CD45.2+GFP+) were isolated by FACS. RFP+ and RFP- T cells were separately sorted, stained with sample hashtags individually, and subsequently pooled into a single sample. Library preperation was performed in according the manufacturers protocol of the 10X genomics Chromium Single Cell 3' kit. Sequencing was performed on Next-Seq550 (Illumina) Library preperation was performed in according the manufacturer's protocol of the 10X Genomics Chromium Single Cell 3' kit (V3)
GEO
GSM5177035