16S rRNA gene V4 amplicon sequencing of the bacterial communities of a dairy bedding sand recycling system
PCR amplification of the bacterial 16S rRNA gene V4 region was performed using univeral primers (Kozich et al. 2013). Amplicons equimolarly pooled and sequenced on an Illumina MiSeq using 2x250bp paired-end sequencing.
SUB9450126
WHP8