GSM5182581: 3_KO814; Mus musculus; RNA-Seq
We isolated proximal colon epithelial cells from 4 Gpr35flfl-Vilcre+ and 4 Gpr35flfl-Vilcre- female littermates at a steady state. Crypts were dissociated to single-cell suspensions. Single-cell suspensions were stained with Epcam+ (BioLegend #118213), CD45- (eBioscience #64-0451-82) and CD31- (BioLegend #102414) and sorted into an Eppendorf tube containing 50 μL of 1X PBS with 0.4% BSA and 5% FBS. Sorted cell suspensions from each mouse were loaded into a single well of a 10X Genomics Chromium Single Cell Controller. Single-cell capture and cDNA and library preparation were performed with a Single Cell 3' v3 Reagent Kit (10X Genomics) according to manufacturer's instructions.
GEO
GSM5182581