LiCAT-seq of Homo Sapiens: hESCs
Differentiated cells, hESCs, sperm and oocytes for LiCAT-seq were lysed with the following mild lysis buffer (10mM Tris-HCl pH7.5, 10mM NaCl, 0.25l RNase-inhibitor, 0.5% IGEPAL). After lysis and physical separation, nuclei were subjected to tagmentation, and the supernatant (containing the RNA content) were subjected to Smart-seq2 protocol.
SUB4589904
hESCs_LiCAT_A_Rep1