JZ_D_46
The V3V4 regions of the 16S rRNA genes in each gut microbiota DNA were amplified using the primers 341F806R. Then, the obtained PCR products were purified, quantified, and mixed in equal amounts to construct the sequencing libraries. The quality of the sequencing libraries was then determined using an Agilent Bioanalyzer 2100 system and Qubit@ 2.0 Fluorometer (Thermo Scientic, USA). Finally, the Illumina NovaSeq 6000 platform was applied to sequence these libraries with the 250 bp paired-end strategy.
SUB9273843
JZ_D_46