PBMCs of homo sapiens [sncRNA-seq], S130_1
The DNBelab C Series Single-Cell Library Prep Set (MGI, #1000021082) was utilized as previously described (Liu et al., 2019). In brief, single-cell suspensions were used for droplet generation, emulsion breakage, beads collection, reverse transcription, and cDNA amplification to generate barcoded libraries. Indexed scRNA-seq libraries were constructed according to the manufacturers protocol. The DNA nanoballs (DNBs)-based libraries were sequenced by the ultra-high-throughput DIPSEQ T1 sequencer at China National GeneBank (CNGB).
SUB9391301
S130_1