paddy soil sample3 for AOA
The PCR amplification of AOA and AOB amoA genes were undertaken on an ABI9701 thermocycler (Applied Biosystems, California, USA),PCR amplicon libraries of AOA and AOB amoA genes were prepared by combining the three independent PCR products for each sample. The PCR products from the different samples were quantified using the QuantiFluorTM-ST System (Promega, Wisconsin, USA) and then pooled at equal concentrations. Amplicon sequencing was performed on a Roche GS FLX Titanium platform (454 Life Sciences, Branford, CT, USA) at Majorbio BioPharm Technology Co., Ltd, Shanghai, China.
SUB1610944
AOA3