WES of 2XSB MPNST cell line
Briefly, the overall flow was as follows: DNA (200 ng) was sheared to an average size of 350 bp using a Corvaris S220 sonicator (Woburnm, MA). Sheared DNA was exome enriched followed by next generation sequencing (NGS). Enriched libraries were prepared as described in the Agilent (Santa Clara, CA) SureSelect XT Human All Exon V5+UTRs Target Enrichment System for Illumina Multiplexed Sequencing manual. Next Generation sequencing was performed following the manufacturers protocol (Illumina, San Diego, CA).  Paired-end (2X125 cycles) sequencing was performed on the Illumina HiSeq2500 utilizing version 4 reagents and software. 15Gb of data was be obtained to ensure an overall average of 100X coverage. Upon completion of the sequencing run, basecalls were converted to Fastq files using the Illumina Bcl2 software.
SUB9058528
2XSB cell line WES