NextSeq 500 paired end sequencing; Mir142 loss unlocks IDH2R140-dependent leukemogenesis through antagonistic regulation of HOX genes
Mir142 loss unlocks IDH2R140-dependent leukemogenesis through antagonistic regulation of HOX genes
FACS sorted cells from Transplanted mice 22-400 cells were directly sorted into 800μl Trizol® and frozen at -80oC. RNA was extracted in the aqueous phase after addition of 160ul chloroform, precipitated with an equal volume of isopropanol supplemented with 20μg linear polyacrylamide and washed once in fresh 80% ethanol. The RNA pellet was re-suspended in 3μl water at 50oC for 10 minutes and then kept on ice.  RNA was then reverse transcribed and amplified using SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (634891) and libraries generated using Nextera® XT library preparation kit (Illumina, FC-131-1096).
UCL Cancer Institute
E-MTAB-8042:HOM_IDH_KLS_5_p