GSM5077610: N1_a549_siCTRL_rep1; Homo sapiens; RNA-Seq
Total RNA was obtained from A549 cell line. The size profiles of the individual libraries were analyzed with LabChip GX II using a DNA High Sensitivity kit (both PerkinElmer, USA). Libraries were quantified on a Qubit with the DNA High Sensitivity kit (Life Technologies). Library preparation was performed using SMARTer smRNA-Seq kit (Clontech/Takara, USA) protocol with minor changes to increase the chance of identifying also pri-miR in addition to miRNAs. RNA-Seq on a Illumina HiSeq 2500  instrument, in rapid run mode, using a 100-bp, dual-indexed, single-end sequencing configuration
GEO
GSM5077610