Sequencing of ADE2 insertions in ADE2 mutant population
A 307-322 bp region (depending on the number of insertions introduced) was PCR amplified with primers that added five degenerate bps on each end. This was followed by 2x250 paired-end sequencing on an Illumina HiSeq4000.
SUB3075706
eMAGE_ADE2_insertions