GSM5343713: siNC_RNAseq_HEK293FT_rep1; Homo sapiens; RNA-Seq
For mRNA-seq, total RNA was isolated from human cells with an RNA purification kit (Qiagen, 74134) and genomic DNA was removed by genomic DNA binding columns in the kit. For RNA-seq, two μg of total RNA was used to start RNA-seq library construction. Poly(A)-containing mRNA was isolated by poly-A selection beads and further reverse transcribed to cDNA. The resulting cDNA was ligated with adapters, amplified by PCR, and further cleaned to obtain the final library. Libraries were sequenced on an Illumina Hiseq machine (Novogene) to obtain 150 bp paired-end reads.
GEO
GSM5343713