GSM3665882: HR14m1; Chenopodium quinoa; RNA-Seq
Total RNA was extracted with Qiagen's RNeasy Plant Mini Kit, including the RNase-free DNase Set. Libraries were constructed by Novogene (https://en.novogene.com/) with NEB Next® Ultra™ RNA Library Prep Kit: mRNA from eukaryotic organisms is enriched using oligo(dT) beads. the mRNA is fragmented randomly by adding fragmentation buffer, then the cDNA is synthesized by using mRNA template and random hexamers primer, after which a custom second-strand synthesis buffer (Illumina) , dNTPs, RNase H and DNA polymerase I are added to initiate the second-strand synthesis. Second, after a series of terminal repair, A ligation and sequencing adaptor ligation, the double-stranded cDNA library is completed through size selection and PCR enrichment
GEO
GSM3665882