GSM5179463: CTRL_1_NOTCHWT_TCFWT [Hi-C]; Mus musculus; Hi-C
Low input in situ Hi-C was performed on 150,000 LSKs with Arima-HiC Kit (Arima) according to the manufacturers' protocols. DNA fragmentation was performed on Covaris E220 Evolution sonicator (Covaris Cat# 500429) with default settings for 400bp DNA size distribution. Libraries for sequencing were prepared with Swift Biosciences Accel-NGS 2S Plus DNA Dual Indexing Library Kit (Swift Biosciences Cat# 21024 and #28096). Size selection steps were performed with Agencourt AMPure XP magnetic beads (Beckman Coulter). Library amplification was performed following DNA quantification with Invitrogen Qubit Fluorometer and KAPA Library Quantification Kit (Roche Cat# 07958927001). sequencing protocol: Prepared libraries were sequenced using the sequencing platforms at Novogene with Illumina NovaSeq 6000 S4 and the 150 bp paired-end reads generating around 700 million paired reads per library.
GEO
GSM5179463