Amplicon sequencing of soil microbial community: arbuscular  mycorrhizal fungi
We amplified arbuscular mycorrhizal fungal ITS region using a  nested approach: first a sequence of >2 kbp spanning the whole ITS rDNA  region was amplified using a set of partially degenerate primers SSUmAf1-2  and LSUmAr1-4. The resulting PCR product was then used as a template to  amplify ITS2 using the 5 modified primers 5.8S and ITS4 .
SUB8323187
SAMPLE.597.ML.1