GSM2801040: 6mA-IP_rep1; Homo sapiens; MeDIP-Seq
Genomic DNA was extracted using Qigen DNA Extract Kit (Cat#). Lysates were harvested  from sonicated genomic DNA  Immunoprecipitated with 6mA antibody Libraries were constructed according to manufacturer’s instructions of NEB Next DNA library prep kit (EE7370L ). Briefly, Harvested DNA was ligated to adaptor using NEB Next DNA library prep kit. Library concentration was quantified by Qubit® 2.0 Flurometer (Life Technologies, CA, USA) and quantitative PCR, and the insert size was assayed on Agilent Bioanalyzer 2100 system. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumia) according to the manufacturer’s instructions. After cluster generation, the library preparations were sequenced on an Illumina Hiseq X10 platform and 150bp paired-end reads were generated.
GEO
GSM2801040