Cultivated annual Ashwagandha
Whole genome sequencing (WGS) library was prepared with Illumina-compatible NEXTflex Rapid DNA sequencing kit (BIOO Scientific, Austin, Texas, U.S.A.) at Genotypic Technology Pvt. Ltd., Bangalore, India. Briefly, DNA was shearedusing Covaris S2 sonicator (Covaris, Woburn, Massachusetts, USA) to generate approx. fragment size distribution from 300 bp to 500bp.The fragment size distribution was checkedon Agilent TapeStation and subsequently purified using Highprepmagnetic beads (Magbio). Purified fragments were end-repaired, adenylated and ligated to Illumina multiplexbarcode adaptorsas per NEXTFlex Rapid DNAsequencing kit protocol. Adapter-ligatedDNA was purified usingHighprepbeads. Resultant fragments were amplified for 5cyclesof PCR using Illumina-compatible primers provided in the NEXTFlexRapid DNA sequencing kit.Final  PCR  product  (sequencing  library)  was  purified withHighprepbeads, followed  by  library quality  control  check. Illumina-compatible  sequencing library  wasquantified  by  Qubit  fluorometer (Thermo  Fisher  Scientific, MA,  USA)  and  its  fragment  size  distribution  was analyzed  on Agilent 2200 Tapestation.
SUB4426480
CWS11