GSM1575763: WT_H2; Glycine max; RNA-Seq
Seeds were cut in half along the cotyledonary axis.  One half was frozen in liquid nitrogen and ground to a powder, then seed total RNA was extracted using the Qiagen Rneasy plant kit (catalog # 74904) and digested on column with Invitrogen Dnase 1 (catalog # 18068-015). cDNA libraries were constructed as described in the Illumina TruSeq RNA Sample Prep version 2 protocol utilizing Superscript II Reverse Transcriptase from Invitrogen (catalog # 18064-014). Illumina unique adapters were ligated to each library and quality control was performed on the agilent 2100 bioanalyzer before sequencing.
GEO
GSM1575763