GSM4815662: at-endosperm.RNAseq.ref6-1.replicate3; Arabidopsis thaliana; RNA-Seq
Total RNA from endosperm was extracted using MagMAX™ Plant RNA Isolation Kit (Thermo Fisher Scientific) according to the supplier's instructions for RNA extraction from plant seeds. mRNA was isolated from total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Kit (NEB) and cDNA libraries were constructed using the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (NEB). Libraries were sequenced on Illumina NovaSeq platforms with paired-end 150 bp read length through Novogene mRNA sequencing services.
GEO
GSM4815662