GSM5342045: H3K4me3_WT-PBS [ChIP-seq-H3K4me3]; Mus musculus; ChIP-Seq
Microglia were isolated by mechanical tissue dissociation and Percoll gradient centrifugation at 4°C followed by flow cytometry of single, DAPI(-),CD11b(+),CD45(+),Ly6c(-) cells. After formaldehyde fixation and cell and nuclear lysis, chromatin was sonicated. Fragmented chromatin was precleared with protein A agarose beads and incubated over night with antibodies binding to the investigated histone modifications. Immune complexes were precipitated with protein A  beads. Chromatin was eluted, decrosslinked and DNA was purified with the GeneJET PCR purification kit. Sequencing libraries were generated from the purified DNA by MicroPlex Library Preparation Kit according to the manufacturer's protocol and sequenced on a Illumina HiSeq 2500..
GEO
GSM5342045