total RNA: EBOV-Minigenome with delta5-spacer transfected HEK293 cells
Poly(A)+ RNA was isolated from the total RNA samples. First-strand cDNA synthesis was primed using a N6 randomized primer. After fragmentation, the Illumina TruSeq sequencing adapters were ligated in a strand specific manner to the 5' and 3' ends of the cDNA fragments. The cDNA was finally amplified with PCR using a proof reading enzyme. The random-primed cDNA pool was size fractionated in the size range of 300 - 500 bp using a preparative agarose gel.
SUB9183141
delta5_plusVP30_total_T1842_S18