GSM4815572: BmN4_native_H3K9me2; Bombyx mori; ChIP-Seq
ChIP-seq: DNA-protein complexes (either cross-linked with formaldehyde or not) were isolated with specific antibody after chromatin solubilization using MNase and sonication. isolated DNA was purified using phenol/chloroform/isoamyl alcohol extraction method. Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. mRNA-seq: Total RNA was extracted from cells using Trizol reagent. All steps of Illumina library preparation and sequencing were carried out at the Curie Institute's sequencing platform using TruSeq ChIPseq protocol or TruSeq Stranded mRNA protocol. ChIP-seq or mRNA-seq
GEO
GSM4815572