Illumina HiSeq 2000 paired end sequencing; Temporal adaptation of carotid endothelial transcriptome  in hyperlipidemic pigs
Temporal adaptation of carotid endothelial transcriptome  in hyperlipidemic pigs
Animals were placed under general anesthesia and exsanguinated. The common carotid artery was dissected from the surrounding tissue, flushed with cold PBS, and incised lengthwise. Endothelial cells were freshly isolated by using a gentle mechanical scraping procedure. Cells were transferred directly to a lysis buffer containing RNase inhibitors and transported on dry ice. Total RNA was isolated by mirVana miRNA Isolation Kit (Life Technologies). The quality of RNA was evaluated by NanoDrop 1000 Spectrophotometer and TapeStation 2200 (Agilent Technologies). TruSeq RNA Sample prep library construction V2, starting input 200 ng.
University of Pennsylvania
E-MTAB-3742:HFHC 2W D RNA