GSM5077822: T1 sample 1 snRNA-seq; Salmo salar; RNA-Seq
Nuclei were released by detergent mechanical lysis, then samples were homogenized (30s) and nuclei isolated by sucrose gradient as Matson et al (2018) Libraries were created using Chromium Single Cell 3ʹ GEM, Library & Gel Bead Kit v3 (10x technologies) by University of Manchester genomic technology core facility (UK). Raw data was converted to counts per cell using Cell Ranger (10x Technologies, ver. 3.1.0) and processed using NCBI annotations. The R package Seurat (ver. 3.1.5) was used to perform an integrated analysis using all snRNAseq data
GEO
GSM5077822