Illumina HiSeq 2000 paired end sequencing; IMPACT OF INTERLEUKIN 10 DEFICIENCY ON INTESTINAL EPITHELIUM RESPONSES TO INFLAMMATORY SIGNALS
IMPACT OF INTERLEUKIN 10 DEFICIENCY ON INTESTINAL EPITHELIUM RESPONSES TO INFLAMMATORY SIGNALS
Small intestinal organoid cultures were growing in 24-well plates SI organoids were growing in matrigel with Intesticult medium and they were split at least once before the experiment addition of 40ng/ml of recombinant mTNF in the culture medium Strand-specific sequencing libraries were prepared with the TruSeq stranded Total RNA kit (Illumina) from 1 Âµg total RNA of each sample and sequenced on an Illumina HiSeq2000 (100-nucleotide paired-end reads).  RNA extraction and purification from enteroids was performed using the RNeasy mini-kit (Qiagen), as per manufacturer instructions.  Strand-specific sequencing libraries were prepared with the TruSeq stranded Total RNA kit (Illumina)
Biology Department, College of Science, UAE University
E-MTAB-10217:D4582_p