RNAseq of Senecio  spp (young plants)
Total RNA extacted  and treated with DNase, mRNA was selected and converted to cDNA. Second  strand cDNA synthesis to incorporate dUTP. The cDNA was end-repaired,  A-tailed and adapter-ligated. Prior to amplification, samples underwent  uridine digestion. The prepared libraries were size selected, multiplexed and  QCed before paired end sequencing.
SUB5841939
BN894