GSM3723419: Adult male hypoxia RNAseq 1; Rattus norvegicus; RNA-Seq
Total RNA was isolated from heart tissue using TRIzol RNA Isolation reagent. Briefly, each solid tissue was homogenized in 1 mL TRIzol and the lysate was then extracted with chloroform and centrifuged at 14 000 rpm for 15 min at 4°C. Finally, total RNA was precipitated and washed thoroughly with 70% ethanol, airdried and reconstituted in nuclease free water. RNA quality was assessed using the 2200 TapeStation. Heart samples had an average RIN number of 9.5. RNA-seq libraries were constructed using the Ovation Universal RNAseq System.
GEO
GSM3723419