GSM2630057: UNGKO control 1; Homo sapiens; OTHER
DNA was extracted by Universal Genomic DNA Kit (CWbiotech, CW2298). Genomic DNA was digested to 100-500 bp and purified with Agencourt AMPure XP beads (BECKMAN COULTER) following Spin-6 column (Bio-rad) purification. 2 μg DNA fragments and 15 pg spike_in sequences were used for end repair with NEBNext® End Repair Module (NEB, E6050) and 1 μl E.coli ligase was added to repair nicks in DNA. DNA was then purified with 1.8×AMPure XP beads. dA was added to the 3’ end of double-stranded DNA by NEBNext® dA-Tailing Module (NEB, E6053) and purified with 1.8×AMPure XP beads.   Damages that may interfere the following labeling step were repaired. DNA was purified and subjected to in vitro BER labeling. After purification, fragments labeled with biotin were enriched by streptavidin C1 beads (Invitrogen) following the manufacturer’s guidelines.  Y adaptor  was ligated to double-stranded DNA on streptavidin C1 beads so that free adaptor can be removed by beads washing. DNA was then eluted from beads by 95 °C 3 min using deionized water. Eluted DNA was subjected to PCR amplification and sequencing.
GEO
GSM2630057