GSM3414869: BRAF1_EV [BiSulfite-seq]; Mus musculus; Bisulfite-Seq
DNA/RNA was extract using Allprep kit mini or micro (Qiagen) RRBS was performed as previously reported (Petkovich et al., 2017). RRBS library was constructed following exactly the same protocol. Sequencing libraries were sequenced on the Illumina HiSeq 2500 platform using 50bp single-end sequencing with v4 reagents (Bauer Core Facility, Harvard University). Since RRBS libraries generate low complexity libraries, the samples were spiked with ∼10% phiX. Ligation reactions were set up by adding 4.8 µl of T4 Ligation buffer, 1.6 µl of T4 DNA Ligase (New England Biolabs), and 3.2 of µl TruSeq Nano DNA LT adapter from Illumina that was diluted 1:20, and 6.4 µl of nuclease-free water to the DNA samples. We used 4–6 different indexed adaptors per library.
GEO
GSM3414869