WGS-LC99-N
DNA were isolated using the DNeasy Blood & Tissue Kit (QIAGEN) supplemented with the RNase Inhibitor (Invitrogen) treatment following the manufacturers protocol. For DNA library preparation, 500 ng DNA of each sample was fragmented using the Ultrasonic DNA shearing (S220, Covaris). The desired length range of the DNA fragments was purified using the AMPure XP beads (Beckman Coulter) and checked with the Agilent 2100-chip (Agilent). The recovered DNA was used to generate DNA libraries using the Ultra II DNA Library Prep Kit (NEB). The paired-end (2150-bp) whole-genome sequencing (WGS) was conducted on the NovaSeq6000 (Illumina).
SUB9312927
L23