The transcriptome of Schizothorax waltoni
The preparation of transcriptome library was started with 10g of total RNA from each sample using an mRNA-Seq Sample Prep Kit. After purifying and fragmenting the mRNA, first strand cDNA was used to synthesize the second strand. Following end repair and adding A bases, the adaptors were ligated to the ends of the cDNA fragments. After fragment size-selection on gels, suitable cDNA fragments were enriched by PCR. Finally, quality control and quantification of the library was assessed using Agilent 2100 Bioanalyzer and Qubit. Clusters were generated in a cluster station system and sequenced using the Hiseq2000.
SUB5303452
Schizothorax waltoni