ddRAD-seq of Darjeeling tea cultivar: Kopati-1/1
a) Double digestion of Genomic DNA (1 microgram) was done using Sphl and Mluc1 Restrictionenzymes.b) Cleanup of the digested product using Ampure beads.c) Ligation of P1 (Barcoded) and P2 adaptors using T4 DNA ligase.d) Pooling and cleanup of the ligated product.e) Size selection of the product was done after 2% agarose gel electrophoresis.f) PCR amplification to enrich and add the Illumina specific adapters and flow cell annealing sequences.g) QC check on bio analyzer.h) Final pooling and sequencing.
SUB7901112
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