RNA-Seq of Arabidopsis: leaf tissue in vegetative stage
About 200 mg leaf tissue was gridded with liquid N2 and further homogenized in TRIzol Reagent (Invitrogen, #15596018). A total amount of 1.5 g RNA per sample was used for oligo(dT)-magnetic bead-based purification of polyA-contained RNA (Invitrogen, #61002). RNA-seq libraries were made with NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, #E7770). All procedures were following each manufactural instruction.
SUB9310440
s158