patient 43 Fluoride_Arginine
16S rRNA Gene Sequencing. 16S rRNA gene libraries were created as described by Caporaso et al. [62], employing primers that amplify the 16S rRNA gene variable region 4 (V4) region of all bacteria and archaea. A mock community and no template control were included. Amplicons were quantified using the KAPA Library Quantification Kit for Illumina Platforms (KR0405, KAPABiosystems, Wilmington, MA), normalized and pooled. Library quality was assessed by Agilent Bioanalyzer chip then the library was prepared for sequencing on the Illumina MiSeq instrument using a MiSeq v3 Reagent kit (2 x 300 bp reads)
SUB9171747
16S-43FSCF-e-MS643_S84_L001_R1_001.fastq.gz