RNA-Seq of BACH2 Edited Cells, LTR-MSD
Approximately 1 x 10e6 CD4+ T cells from a late culture timepoint (day 48 post-editing) were collected for RNA extraction (Qiagen) and poly-A selected mRNA library preparation (TruSeq RNA Library Prep Kit v2; Illumina). Cells were collected from 2 editing experiments. 33-41 million raw paired-end reads per sample were generated. Reads were mapped to a custom hg38 human reference genome which divided the BACH2 locus into 2 regions: BACH2_5p reads mapping upstream of the target site (hg38 gene position: 115,649) and BACH2_3p reads mapping downstream of the target site.
SUB9742073
LTR-MSD_1