NextSeq 500 sequencing
RNA­seq of Bacillus thuringiensis sv. galleriae 4G5 infected with the Waukesha92-like phage, 120 minutes post infection, without addition of the arbitrium peptide to the growth medium
Ribosomal RNA was depleted by using the Ribo­Zero rRNA Removal Kit (epicentre, MRZB12424). Strand­specific RNA­seq was performed using the NEBNext Ultra Directional RNA Library Prep Kit (NEB, E7420) with the following adjustments: all cleanup stages were performed using ×1.8 SPRI beads, and only one cleanup step was performed after the end repair step.
WEIZMANN INSTITUE OF SCIENCE
ena-EXPERIMENT-WEIZMANN INSTITUE OF SCIENCE-11-03-2019-13:10:39:972-5