faecal metagenome of cygnus olor, sample UTMF1193
Encapsidated viral nucleic acids were enriched from faecal material by entrifugation and by DNase treatment. DNA and RNA nucleic acids were extracted, reverse transcription was performed using non-ribosomal random hexamers. Second strand DNA synthesis was performed with Klenow fragment DNA polymerase. Nucleic acids were purified by phenol/chloroform extraction and ethanol precipitation. Samples were barcoded and paired-end library preparation was performed for all samples using an Illumina Nextera XT kit.
SUB8750037
UTMF1193