GSM2629471: LAM_1; Mus musculus; RNA-Seq
Mouse E12 spinal cords were dissociated and plated in 6-well tissue culture treated plates (Thermo Scientific, Logan, UT) at 500,000 cells in 2 ml of media coated as described above: PDL, PDL & laminin, PDL & laminin & myelin, and PDL & myelin. In addition, wells coated with myelin without cells were collected to control for potential RNA presence in the myelin coating. Cells were cultured for 48 hours prior to RNA extraction. RNA was collected using Absolutely RNA Nanoprep Kit (Agilent, Santa Clara, CA) according to manufacturer’s instructions. N = 3 biological replicates per condition. cDNA was generated using the TruSeq RNA vs library prep kit (Illumina). Paired-end libraries for multiplex sequencing were generated.
GEO
GSM2629471