GUIDEseq_HEK293T_RNP
One million HEK293T cells were transfected with 12 g HIFI Cas9, 4 g WAS gRNA #1 and 5 pmol of dsODN using Lonza Nucleofector 4-D (program CM-137). At 48 hours post transfection, genomic DNA was extracted and sheared using a Covaris S220 Focussed-ultrasonicator to an average length of 500 bp. After end-repaired, A-tailed and ligation with adapters containing 8-nt random molecular index, the DNA library was sequenced using Illumina Miseq.
SUB7556948
RNP