RNA-seq analysis of hiPSC cultured in complete or arginine deprived media
Human pluripotent stem cells (PSCs) were cultured on matrigel-coated (Corning) plates with StemFit® AK (Ajinomoto Co., Inc, Tokyo, Japan). For arginine deprivation, cells were cultured with arginine-deprived StemFit® AK medium. Total RNA was extracted with RNeasy plus Mini Kit (Qiagen). Sequencing libraries were produced using TruSeq® RNA Sample Preparation Kits v2 (Illumina) and sequenced on an Illumina® NextSeq 500. All procedures were carried out according to the manufacturer's instructions.
University College London
MIFF3 deltaArg 8hr biological replicate 1