Metagenome sequencing of ANME-1 single cell AG-392-L03
sediment samples were diluted and cells were separated from the sediment by a slow centrifugation step. Afterwards, single cells were sorted by high-speed fluorescence-activated and droplet-based cell sorting (FACS) into three 384-well plates and cell lysis followed using five freeze-thaw cycles and KOH treatment. Then, whole-genome amplification via multiple displacement amplification (MDA) was performed with subsequent cell screening and genome sequencing. Cell screening identifying archaeal cells affiliated with ANME was performed by 16S rRNA-gene tag sequencing, while libraries for genome sequencing were created with Nextera XT (Illumina) and were sequenced with NextSeq 500 (Illumina). Reads were quality trimmed using Trimmomatic v0.32 with the following parameters:  -phred33 LEADING:0 TRAILING:5 SLIDINGWINDOW:4:15 MINLEN:36  and screened for contamination against the human genome assembly GRCh38.
SUB3609593
AG-392-L03