GSM3455669: RNA-seq_WT_Cortex_Bound_R1; Mus musculus; OTHER
To prepare total RNA, 350 μl of Qiagen RLT buffer was added to the RiboTag immunoprecipitated samples or to the input samples. Total RNA was prepared according to manufacturer's instructions using the RNeasy Plus Micro kit (Qiagen), and the quantity and quality of the RNA were assessed using a Bioanalyzer (Agilent). For RNA-seq, total RNA was converted to cDNA and amplified (Ovation Ultralow System V2-32, 0342HV, NuGEN Technologies, San Carlos, CA). Amplified cDNA was fragmented, end-repaired, linker-adapted, and single-end sequenced for 75 cycles on a NextSeq500 (Illumina Inc.).
GEO
GSM3455669