GSM3453937: HIF1α-mutant 3h hypoxia rep 3; Homo sapiens; RNA-Seq
Cells were washed 3x with PBS and harvested in 1 ml TRIzol Reagent (Thermo Fisher Scientific). Chloroform (0.2 ml, Acros Organics) was added to lysates and after shaking (5s) samples were centrifuged for 18 min at 10,000 x g. The  aqueous phase was mixed with an equal volume of 100% ethanol (500 μl) and RNA was purified using the RNeasy Kit (Qiagen), according to the manufacturer's instructions. Libraries were prepared using the KAPA Hyper Prep kit (Roche) according to the manufacturer's instructions.
GEO
GSM3453937