GSM5078950: GS-A; Homo sapiens; RNA-Seq
We deparaffined 3 slices of 10 µm-thickness from each FFPE-block according to the xylene protocol described in the RNeasy FFPE kit (Qiagen) handbook. Total RNA were obtained after purification and DNase digestion using the RNeasy FFPE kit (Qiagen) following manufacturer's instructions. The starting amount of total RNA was 100 ng following Illumina recommendations to prepare the sequencing library using the TruSeq RNA Exome library kit (Illumina).
GEO
GSM5078950