Kronos_polysome_replicate2
Around 500mg wheat seedlings were harvested and grounded into fine powder in liquid nitrogen. Precooled polysome extraction buffer was added and  incubated on ice for 30 minutes for full lysis. The lysate was applied to centrifugation with 13200 rpm for 15 min at 4 degree, supernatant was transferred to sucrose gradient with salt buffer. Polysomes were fragmented by 4 hours of centrifugation at 40000 rpm. Low to high sucrose gradient was collected, RNA was extracted from the fractions corresponding to the polysomes and applied to library generation.
SUB9529120
KRONOS_POLYSEQ_B2