Aphidius ervi SA strain genome
DNA was extracted from adult haploid males of A. ervi in seven sub-samples (ca. 120 males each). Total DNA was extracted using the DNEasy Plant Mini Kit (QIAGEN", Hilden, Germany) following the manufacturers' instructions). DNA was quantified by spectrophotometry (Epoch Microplate Spectrophotometer, Biotek) and fluorometry (Qubit 3.0; Qubit DNA High sensitivity Assay Kit, Invitrogen) and DNA quality was assessed on 1% agarose gel electrophoresis. DNA samples were sent in dry ice to MACROGEN (Seoul, South Korea). Extracted DNA was used to produce Illumina paired-end (PE) and mate-pair (MP) libraries for sequencing. Mate Pair library (5kb insert size) was constructed from one of the seven sub-samples (120 individuals, 1ug DNA) sheared by ultrasonication (Covaris, Woburn, MA) company, average sheared insert size: 5kb)
SUB5848347
Ae-SA-5kb