GSM2101987: RD_fibersMMP1_4h_1_TGTCGG; Rattus norvegicus; RNA-Seq
Cells adhering to native or degraded ECMs were directly lysed in the presence of QIAzol and total RNA was extracted with the miRNeasy Mini Kit (Qiagen). The RNA integrity number (RIN) was determined using the TapeStation System (Agilent Technologies). Quantity of RNA was determined by Qubit Fluorometric Quantitation kit (Life Technologies). 3' end mRNA libraries were prepared for sequencing using a method developed at Dr. Ido Amit's lab, Weizmann Institute of Science 3' RNA-seq for digital gene expression quantitation
GEO
GSM2101987