16S rRNA amplicon libraries of lake and sediment samples
Total DNA was submitted to the Center for Bioinformatics & Functional Genomics at Miami University (Oxford, OH, USA) where amplicons were sequenced using MiSeq Illumina 2 × 300 bp chemistry with the primers 515Ff and 806rB targeting V4 hypervariable region of bacterial and archaeal 16S SSU rRNA gene sequences (Caporaso et al., 2012; Apprill et al., 2015). Each sample was sequenced once. Mothur (ver. 1.39.1; (Schloss et al., 2009)) was used to for post sequencing processing following the MiSeq SOP (Kozich et al., 2013).
SUB2675261
150801IS