GSM5179334: rip 4h WT rep2; Danio rerio; RIP-Seq
RIP was carried out as previously described (Despic et al., 2017). flag-ddx3xb mRNA injected embryos were collected at 4 hpf and 6 hpf. 400 zebrafish embryos were irradiated twice with 0.8 J/cm2 (Stratalinker 2400, Stratagene), lysed, and subjected to mild RNA fragmentation. Crosslinked RNA-proteins complexes were immunopurified using Anti-FLAG M2 Magnetic Beads (Merck, M8823) for 4 h. RNA was extracted and subjected to library construction using Smarter smRNA-Seq kit (Clontech Laboratories Inc)
GEO
GSM5179334