GSM5261836: ChIP-seq G401 IgG rep3; Homo sapiens; ChIP-Seq
For ChIP-seq experiments, chromatin was extracted from nuclear pellets and frozen until ready for immunoprecipitation with antibodies. Immunoprecipitated DNA was extracted using a PCR purification kit. For PRO-seq, run-on biotinylated RNA from nuclei were extracted using trizol and streptavidin Dynabeads, and then processed and continually purified through multiple rounds of streptavidin Dynabead addition. For RNA-seq, Cells were collected in Trizol and RNA was purified with Direct-Zol RNA Miniprep kit (Zymo) with on-column DNaseI treatment. Libraries were prepared for sequencing using standard Illumina protocols
GEO
GSM5261836