GSM5176576: Input sample; Mus musculus; ChIP-Seq
Mammary tissue was dissected into small pieces and crosslinked using formaldehyde (1% w/v, methanol-free) followed by homogenization and chromatin preparation using a Covaris sonicator (Covaris, Woburn, MA). Chromatin was immunoprecipitated using an appropriate antibody, washed, reverse-crosslinked, and DNA isolated using the phenol-chloroform method. Precipitated DNA was QC'ed by quantitative PCR using Mouse ChIP Control qPCR Primer Sets (Active Motiff, Carlsbad, CA). For ChIP-sequencing, 10ng precipitated DNA was used to prepare the library using Illumina TruSeq ChIP library preparation kit. Libraries were quantified for cluster generation using KAPA Library Quantification Kit (Kapa Biosystems, Wilmington, MA). Sequencing was performed using Illumina HiSeq2500 using 50-bp single-end rapid run format.
GEO
GSM5176576