The WNT targets in hESCs-derived hindgut cultures are composed of multi-lineage genes. [Endoderm RNA-seq and ChIP-seq data sets]
H9 embryonic stem cells were differentiated into definitive endoderm and then treated with FGF4/WNT3a in order to generate intestinal organoids as previously described by Spence, et al. Nature 470:105-109(2010) and McCracken, et al. Nature Protocols 6:1920-1928 (2011). In order to identify WNT targets, WNT3a was substituted by WNT antagonist DKK1. Cells were immediately lysed and mRNA was extracted using the Qiagen RNeasy Mini kit according to the manufacturer’s protocols. Libraries were constructed by using standard illumina protocols.
institute of zoology
WNT targets in differetiated endoderm cells