GSM5170244: LS01_emb_RNA_rep3; Caenorhabditis elegans; RNA-Seq
RNA was extracted by freeze cracking by freezing in liquid nitrogen and thawing at 37C water bath five times. RNA was isolated according to the manufacturer's (Invitrogen) Trizol extraction protocol. Purified total RNA was cleaned up through Qiagen RNeasy column. mRNA was purified from .5-10 ug of total RNA using Sera-Mag Oligo(dT) beads (ThermoScientific). cDNA preparation was done in the presence of dUTP to prepare stranded RNA-seq libraries as in PMID:19620212 RNA libraries were prepared for sequencing using standard Illumina protocols
GEO
GSM5170244