GSM2975571: ORG14_POS; Homo sapiens; RNA-Seq
About 16 weeks after the electroporation, the transduced organoids were dissociated in Accutase then passed through a mesh filter to create a single-cell suspension. Cells were collected by centrifugation and resuspended in cerebral organoid differentiation medium and FACS sorted into TRI-zol LE and RNA was purified by Direct-zol™ RNA MicroPrep. cDNA libraries were prepared using the TruSeq RNA Sample Prep kit (Illumina). RNA sequencing was performed using a HiSeq 2500 Sequencing System (Illumina).
GEO
GSM2975571