GSM5222267: WT lipofectamine 2; Homo sapiens; RNA-Seq
At the endpoint, media was aspirated and cells were lysed with hypotonic buffer and dounce homogenization. Nuclei and organelles were pelleted, then RNA from cytoplasmic fraction was isolated using RNeasy (Qiagen; as per manufacturers instructions). RNA integrity was verified by an RNA 6000 Nano Chip, using BioAnalyzer and 2100 Expert software (Agilent Technologies). rRNA was depleted, followed by fragmentation, adapter ligation, PCR amplification, and finally sequencing on Illumina NovaSeq 6000 platform. RNA-Seq directional, paired-end
GEO
GSM5222267