Transcriptome analysis of day 0 5th instar prothoracic glands of Bombyx mori
Day 0 5th instar prothoracic glands of Bombyx mori were used to investigate the gene activity of the glands on this day. The lllumina® mRNA-Seq Sample Prep Kit was used to process the samples. mRNA was isolated from total RNA using oligo-dT magnetic beads. After fragmentation of the mRNA, cDNA synthesis was performed. This was used for ligation with the sequencing adapters and PCR amplification of the resulting products. Quality and yield after sample preparation was measured with the Advanced Analytical Fragment Analyzer. The size of the resulting products was consistent with the expected size distribution with a broad peak between 200-500 bp on a DNA 1000 chip). A concentration of 17 pM of DNA was used for clustering and DNA sequencing on lllumina cBot and HiSeq2500 (HCS v2.2.58 software).
National and Kapodistrian University of Athens
SAMN03978782