GSM1847257: HCT116_DMSO_Replicate1 (GRO-Seq); Homo sapiens; OTHER
Isolation of nuclei and nuclear run-on was carried out as described in Core et al, Science (2008) . Nascent RNAs were on average approximately 100nt long. The immuno-purified RNA was resuspended in 8.5µl water and 5'- or 3'- adapters ligated using Tru-Seq Small RNA Kit, Illumina. RNAs were reverse transcribed and amplified. The NRO-cDNA libraries were then run on a non-denaturing 1XTBE, 8% acrylamide gel, and cDNAs greater than 90 nucleotides were excised from the gel and eluted, precipitated and sequenced on the HiSeq 2000.
GEO
GSM1847257