GSM4403940: DM.HSPC_RNA-seq_rep1; Mus musculus; RNA-Seq
Total RNA was extracted from freshly isolated cell population, using RNeasy Plus Mini kit (Qiagen) according to the manufacturer's instructions. RNA quantity and quality was measured using a NanoDrop 1000 Spectrophotometer (Thermo Scientific) and RNA 6000 Nano Kit (Agilent Technologies) on an Agilent 2100 bioanalyzer, respectively. 2-5 µg total RNA was processed for ribosomal RNA depletion and then for library preparation using a Rapid Directional RNA-Seq Kit (NEXTflex).
GEO
GSM4403940