GSM3724578: 22q11.180.cortex; Mus musculus; RNA-Seq
Samples were lysed and homogenized in RA1 lysis buffer with 1% β-mercaptoethanol (Macherey-Nagel, Düren, Germany) using a PT 1200 E Polytron® (Kinematica, Luzern, Switzerland). Total RNA was purified using TruSeq® Stranded mRNA Sample Preparation kit according to manufacturer's instructions (Illumina, San Diego, Ca, USA). 3' poly-adenylation purification, adapter ligation, and PCR amplification was carried out using the TruSeq® Stranded mRNA Sample Preparation kit per manufacturer's instructions, with a minimum amount of 160 ng RNA per sample (Illumina)
GEO
GSM3724578