GSM3667165: RPE1_hTERT_Cas9_s2_T25B; Homo sapiens; OTHER
Genomic DNA was extracted from cell pellets using the QIAamp Blood Maxi Kit (Qiagen). Genomic DNA was precipitated using ethanol and sodium chloride, and resuspended in Buffer EB (10 mM Tris-HCl, pH 7.5). gRNA inserts were amplified via PCR using primers harboring Illumina TruSeq adapters with i5 and i7 barcodes (primers listed below), and the resulting libraries were sequenced.
GEO
GSM3667165