DNA-Seq of soil fungi
For each sample, total genomic DNA was extracted from 0.25 g fresh soils with PowerSoil DNA Isolation Kit (MoBio Laboratories, Inc., Carlsbad, CA, USA) follow the protocol. An approximately 300-350 bp region of the Internal Transcribed Spacer 2 (ITS2) region was amplified with forward primer gITS7 (Ihrmark et al., 2012) and reverse primer ITS4 (Gardes & Bruns, 1993). Primer gITS7 contained a unique 12 nt barcode at the 5end for Miseq sequencing detection.
SUB4462158
iB2