GSM3453504: BC-3 DMSO treated Day9 harvest; Homo sapiens; OTHER
Cells pelleted, washed twice with sterile cold PBS and flah frozen in an ethanol-dry ice bath before storing at -80C. gDNA was harvested from frozen cell pellets by following the gDNA extraction protocol mentioned in Manzano et al., Nature Communications, 2018. Protocol followed for PCR amplification and barcoding of sgRNA inserts is mentioned in Manzano et al., Nature Communications 2018.
GEO
GSM3453504