Illumina HiSeq 4000 sequencing; RNA-seq of wildtype mouse small intestinal organoids grown in Matrigel or in/on collagen.
RNA-seq of wildtype mouse small intestinal organoids grown in Matrigel or in/on collagen.
Intestinal crypts were grown in Matrigel for at least 2 passages, then grown either in Matrigel, on Collagen I or in Collagen I. All samples were grown for at least 1 passage in/on collagen before RNA collection. RNA was isolated using NucleoSpin RNA kit (Bioke) according to manufacturer's protocol.  RNA-seq libraries were prepared with the KAPA mRNA Hyper Prep Kit (KAPA Biosystems) using 550 ng of total RNA per sample.
Laboratory for Experimental Oncology and Radiobiology (LEXOR), Center for Experimental Molecular Medicine (CEMM) and Cancer Center Amsterdam, Academic Medical Center, Amsterdam, The Netherlands.
E-MTAB-10082:R19_s