GSM2102811: 30min Total basal 1; Mus musculus; RNA-Seq
Tissue was flash frozen in crushed dry ice, followed by homogenization in TRAP homogenization buffer. Debris was pelleted, RNA was extracted from 10% of the homogenate at this step using a combined Trizol/kit purification while the remaining homogenate was incubated with anti-HA agarose beads overnight at 4C. Beads were washed, followed by removal of RNA from beads with vortexing in buffer RLT from Qiagen RNEasy Micro Kit. This was followed by the standard RNEasy kit protocol. rRNA was first depleted with RiboZero Gold, followed by standard Illumina TruSeq library prep Libraries were made from both TRAP and total RNA, for all basal and LTP samples, for each time point on the same day with the same master mix of reagents. All samples at a given time point were processed, multiplexed, and sequenced together.
GEO
GSM2102811