NextSeq 500 paired end sequencing; CUT&RUN Targeting c-FOS, c-JUN and SOX2 in neurospheres derived from mouse brain cells
CUT&RUN Targeting c-FOS, c-JUN and SOX2 in neurospheres derived from mouse brain cells
100 000 neurosphere cells were harvested for CUT&RUN. Antibodies were incubated overnight at a 1:100 dilution. pA-MNase was used at a concentration of 700ng/mL and digestion was performed for 30 minutes. Phenol:Chloroform. KAPA HyperPrep Kit with KAPA DUI Adapters.
Wallenberg Centre for Molecular Medicine (WCMM), Linkoping University, Linkoping, Sweden  Department of Biomedical and Clinical Sciences, Division of Molecular Medicine and Virology, Faculty of Health Science, Linkoping University, Linkoping, Sweden
E-MTAB-9897:Anti-c-JUN_p