GSM2801114: Jan25_Chip320_Cell_53; Mus musculus; RNA-Seq
Cells were harvested from bone marrow.  Femurs, tibias and iliac crest were harvested immediately after euthanasia and put in cold PBS+2%FBS. Bones were crushed with mortar and pestle, filtered and washed in cold PBS+2%FBS. Ly6Chi monocytes (CD11b+ Ly6G– CD115+ F4/80– Ly6Chi cells) were isolated from the bone marrow of wild-type and Gfi1-Tomato IRF8-GFP mice by FACS sorting on BD FACSAria II with a 100μm nozzle. cDNA was made with Clontech SMARTer® Kit “the mRNA Seq: RT + Amp (1771x), Libraries were constructed using Nextera XT DNA Library Preparation Kit Single cells were separated using Fluidigm C1 system. Single-cell libraries were subjected to paired-end 75 bp RNA-sequencing on a HiSeq 2500 (Illumina Inc.). 96 scRNA-seq libraries were sequenced per HiSeq 2500 gel (~300 million bp per gel).
GEO
GSM2801114