GSM2628234: Mbd1 KO 2; Mus musculus; RNA-Seq
The tissue was dissected and homogenized immediately in RLT buffer (Qiagen, RNeasy Micro Kit) and the tissue lysates were snap frozen and stored in a -80 freezer until use. RNA isolation was performed using the RNeasy Micro Kit (Qiagen). Library synthesis using TruSeq mRNA and total RNA stranded sample preparation kit (Illumina). The libraries were then subject to paired-end and 100 bp deep sequencing on an Illumina Hi-Seq2000 (Illumina).
GEO
GSM2628234