GSM5165987: ChIP_H3K27ac_KE37; Homo sapiens; ChIP-Seq
Leukemia blast cells were prepared by density-gradient centrifugation of bone marrow samples, and CD19-CD14-CD235-CD45+CD7+ cells were further purified by FACS analysis using anti-human antibodies for RNA-seq and Hi-C library preparations. Peripheral blood samples were obtained from four healthy donors under the approval of the ethics committee of Peking University. T cells were purified using the EasySepTM Direct Human T Cell Isolation Kit (StemCell Technology #19661). Hi-C was performed on one million cells/sample according to the BL-Hi-C protocol (PMID: 29158486 ). RNA libraries were prepared for sequencing using standard Illumina protocols. ATAC  was performed on 50,000 Loucy cells according to the Omni-ATAC protocol  (PMID:28846090) with TruePrep DNA Library Prep Kit V2 for Illumina® (Vazyme TD501). H3K27ac ChIP of case 077 was performed on 10^5 cells, according to the protocol of Hyperactive In-Situ ChIP Library Prep Kit for Illumina (Vazyme, TD 901). H3K27ac ChIP of KE-37 was performed on 10^6 cells, according to the protocol of Zymo-Spin ChIP Kit from Zymo Research for Illumina (Zymo Research,D5209).
GEO
GSM5165987