GSM1213029: Fetal; Mus musculus; Bisulfite-Seq
Genomic DNA was extracted from mouse liver samples via proteinase K digestion, followed by phenol/chloroform extraction and ethanol precipitation Briefly, 5 ug of genomic DNA was digested with 300U of MspI enzymes (NEB) in 100μl reactions at 37°C for 16–19h. After purification, the digested products were blunt-ended, and then dA was added, followed by ethylated-adapter ligation. To obtain DNA fractions of 40-120bp and 120-220bp ranges of MspI-digested products, two ranges of 160-240bp and 240-340bp adapter-ligated fractions were excised from a 2% agarose gel, respectively. Bisulfite conversion was conducted using the ZYMO EZ DNA Methylation-Gold Kit ™ (ZYMO) according to the manufacturer’s instructions. The final libraries were generated by PCR amplification using JumpStart ™ Taq DNA Polymerase (Sigma).
GEO
GSM1213029