GSM4812301: wound_scRNAseq; Mus musculus; RNA-Seq
Tissue samples were minced with a razor blade into 1 cm fragments, suspended in enzymatic digestion buffer collagenase and DNase I, incubated with frequent agitation at 37°C for 30 min, and triturated briefly with a 5 ml pipet. Cells in a single-cell suspension were then passed through a 100micron mesh filter, centrifuged, stained with a live/dead stain and anti-CD45 antibodies for FACS sorting of leukocytes, and anti-PDGFRa for cells of fibroblast lineage. Single cell suspensions were loaded onto the 10X Genomics Chromium Controller instrument to generate single cell GEMs. GEM-RT and library construction were performed following the 10X Genomics Protocol. Library fragment size distributions was determined using an Agilent Bioanalyzer High Sensitivity chip, and library DNA concentrations were determined using a Qubit 2.0 Fluorometer (Invitrogen). Libraries were sequencedusing an Illumina HiSeq4000.
GEO
GSM4812301