GSM4815297: omniATACseq_Z1_Ngn1GFPbright_r2; Mus musculus; ATAC-seq
Cells were permeabilized in RSB buffer (20 mM Tris-HCl pH 7.6, 10 mM MgCl2, 20% Dimethyl Formamide containing 0.1% NP40, 0.1% Tween-20, and 0.01% Digitonin), nuclei were pelleted and transposed with Tn5. omniATAC-seq libraries were prepared by amplifying transposed DNA fragments with 2x NEBNext MasterMix using adaptors with unique barcodes in the primers.
GEO
GSM4815297