Amplicon sequence of peripheral blood mononuclear cells :female DRESS patient at age 51
PBMCs immediately lysed in TRIzol.total RNA was extracted and reverse transcribed into cDNA,A set of forward primers, eachspecifictooneorasetoffunctionalTCRV-betasegments,anduniversalreverse primersspecifictotheconstantregionofTCRB,wereusedtogenerateampliconsthat covered the entire CDR3 region.
SUB7027797
WangXJTrizol