GSM5182590: TMX, non-methylated DNA; Mus musculus; MBD-Seq
Genomic DNA was isolated and purified with NucleoSpin Tissure Kit (MACHEREY-NAGEL, Cat# 740952). Purified DNA was sheared with Covaris S220 with microTUBE AFA (Covaris, Cat# 520045). Methylated and non-methylated DNA fragments were separated using EpiXplore Methylated DNA Enrichment Kit (Clontech, Cat# PT5034-2). Libraries were generated with DNA SMART ChIP-Seq Kit (Takara, Cat# 634865). The libraries were selected into 250-350-bp fragments by E-Gel 2% SizeSelect electrophoresis (Invitrogen, Cat# G661012). MBD2-Seq
GEO
GSM5182590