Amplicon seq, adenine base editing, SGCA c.157G>A mutation, off-target 2, human muscle stem cells
PCR amplicon off-target site 2, KIF1A locus. Primers: fwd(5>3) GGCAGGGGACAGAGTAAGAGA / rev(5>3) CACGCCGTCTTCAACATCATC. Q5 DNA Polymerase. Amplicon checked with 2100 Bioanalyzer using DNA 1000 Kit (Agilent).
SUB9312277
SGCAex2-ABE-NGS-19_2020-05-29