GBS of Pisum Sativum
DNA from CREA germplasm collection was extracted from leaf green tissues using the DNeasy Plant Mini Kit (Qiagen) and checked for integrity on 1% agarose gel. DNA quantitation was performed using the Quant-iTTM PicoGreen dsDNA assay kit (Life Technologies). A GBS library was prepared using the ApeKI restriction enzyme, following the protocol described by Elshire et al. Library sequencing was performed using the Illumina HiSeq X platform and paired-end runs (2 x 150 bp).
SUB9383630
plate1_1F11