GSM4989629: 231_con_rep1; Homo sapiens; RNA-Seq
Adherent cells were collected with trypsin-EDTA and suspended with PBS.total RNA was harvested using Trizol (Invitrogen) according to the manufacturer's instructions. Total RNA from each sample was quantified by NanoDrop ND-1000 (ThermoFisher) and the quality of the RNA was assessed with the Agilent Bioanalyzer 2100. Transcriptome libraries from the mRNA fractions were generated following the RNA-Seq protocol (Illumina). Each sample was sequenced in a single lane with the Illumina PE150 Analyzer (Illumina) on a 150-bp paired-end run.   For strand-specific library construction, dUTP method of second-strand marking was employed as described(64). For the data processing, the raw sequencing reads were aligned to human reference genome (hg19) using the splice-aware aligner HISAT2(65). Read counts for each gene were normalized into FPKM (Fragments Per Kilobase of transcript per Million mapped reads) values(66). The cutoff of differential gene expression was FDR <0.05, normalized by the respective control group. Transcriptome libraries from the mRNA fractions were generated following the RNA-Seq protocol (Illumina). lncRNA
GEO
GSM4989629