Nanopore Sequencing of Caenorhabditis elegans
The genomic DNA was concentrated and purified using Zymo Quick-DNA kit (D4068). Size-selection to collect DNA fragments from 10kbp  50kbp was carried out using a Blue-pippin. The sequencing library was prepared using 1D ligation kit (SQK-LSK108) following the standard protocol. DNA was reapaired using the NEBNext FFPE Repair Mix (M6630). After DNA repair, end preparation was performed and the adapter was ligated.
SUB5304724
C. elegans_Georgia_Tech_McGrath_Lab_SAMN11101536