GSM5170171: BS18-wt-HSV-HI; Mus musculus; RNA-Seq
Following transcardiac perfusion with 10 mL cold 1X PBS, brainstems were excised, flash frozen in liquid nitrogen, and transferred to 1 ml TRIzol reagent (Invitrogen) and homogenized at speed 6000 for 30 seconds with a MagNA Lyser Instrument (Roche). Total RNA was extracted as per the Invitrogen's standard protocol for TRIzol RNA extraction. RNA samples were further cleaned-up using the RNeasy Mini Kit (Qiagen #74104) and treated with DNase I, as per the manufacturer's standard protocol. RNA integrity was assessed using a Bioanalyzer RNA Pico kit (Agilent). rRNA depletion and library preparation was performed using the KAPA Stranded RNA-Seq kit (Roche).
GEO
GSM5170171