GSM4403022: 5 months SYN Rep 2; Mus musculus; RNA-Seq
Synaptosomes were extracted from cerebral cortices of different ages as previously described (Gray & Whittaker, 1962; Moczulska et al., 2014). Total homogenate samples were kept before proceding with synaptosome enrichment. Synaptosome samples were processed for RNA extraction according to (Smalheiser et al., 2014), and RNA was then extracted from the appositely prepared synaptosome samples and from TH samples through acidic phenol-chloroform extraction according to (Chomczynski, 1993). 10ng of RNA was used for library preparation, which was done using the SMARTer Stranded Total RNA-Seq Kit – Pico Input Mammalian (TaKaRa, #635006) according to the manufacturer's instructions. The obtained libraries are depleted for 18S and 28S rRNAs
GEO
GSM4403022