GSM2803008: Fto_TG_mouse_hippocampus_RNA-seq; Mus musculus; RNA-Seq
For RNA-seq, RNA was isolated from hippocampus sample from 4 weeks old Prrc2a flox/flox and Prrc2a flox/flox; Olig2-cre mice or 4 weeks old wt and Fto Tg micemice  using the TRIzol (Invitrogen) reagent by following the company manual. mRNA was isolated by using Dynabeads mRNA Purification Kit (Invitrogen).  For all samples the RNA integrity was checked using an Agilent Bioanalyzer 2100. Approximately 100ng of mRNA was used for library preparation using a TruSeq™ RNA Sample Prep Kit v2 (Illumina, San Diego, CA, USA) according to the manufacturer’s protocol.The libraries were sequenced using HiSeq3000 (Illumina) in single-read or paired-read mode, creating reads with a length of 101 bp.
GEO
GSM2803008