Illumina HiSeq 2500 paired end sequencing; RNA-seq in GR18 cell line to identify genes regulated by the Glucocorticoid Receptor (24h treatment)
RNA-seq in GR18 cell line to identify genes regulated by the Glucocorticoid Receptor (24h treatment)
RNA was isolated from 1-2 million cells using an RNeasy kit from Qiagen. GR18 cells where grown to approximately 90% confluence according to standard protocols in DMEM medium with 5% FBS. Cells were treated for 24 hours with 1uM Dexamethasone or 0.1% ethanol as vehicle control Prior to reverse transcription, poly adenylated RNA was isolated using oligo d(T) beads. Sequencing libraries were prepared using the TruSeq RNA library Prep Kit (Illumina)
MPIMG
E-MTAB-7745:RNA-seq_DEX_24h_rep3_p
Max Planck Institute for Molecular Genetics
E-MTAB-7745:RNA-seq_DEX_24h_rep3_p