GSM5223076: P0_WT_593_605_file4; Mus musculus; RNA-Seq
Prefrontal cortices from P0 mice were dissected and dissociated into single cells and fixed in ice-cold 80% methanol and barcoded using the ECCITE-Seq scheme with an amine group at the 5' end. We pooled ~20.000 cells from two Myt1l (+/+), (+/-), and (-/-) animals, respectively. Each pool was loaded into an individual lane of the Chromium Controller (10x Genomics). Chromium Single Cell 5' v1 reagent kit was used to process samples according to the manufacturer's instructions, with modifications in the cDNA and library preparation steps suggested by the ECCITE-Seq protocol to generate the barcoded oligonucleotide libraries in parallel. single-cell RNA-seq
GEO
GSM5223076