GSM5170643: Healthy control - C3; Homo sapiens; RNA-Seq
Initial RNA extraction was performed using the Tempus™ Spin RNA Isolation Kit (Ref. 4380204, ThermoFisher Scientific, USA) following the manufacturer's recommendations. The total RNA obtained was quantified and evaluated using the Agilent RNA 6000 Pico microfluidics chip (reference 5067-1513) in the Bioanalyzer 2100 equipment (Agilent Technologies, Palo Alto, Calif.) following the manufacturer's instructions. Library was prepared on 40 ng of total RNA using the AmpliSeq Transcriptome Kit v2 (reference 4475936, Thermo Fisher Scientific, USA) in combination with barcodes Ion Xpress™ 01-16 Kit (4471250AB, Thermo Fisher Scientific, EE.) following the manufacturer's specifications.
GEO
GSM5170643