GSM5170104: ileal crypt cells, CPT3; Mus musculus; RNA-Seq
mice ileum was dissected, flushed, opened and incubated in cold PBS containing 20 mM EDTA for 90 min followed by shaking. Incubation (30 min for each time) and shaking were repeated for two more times, and the supernatant was filtered through a 70-μm strainer and centrifuged at 350 g, 4℃ for 5 min to collect crypts. Isolated crypts were then incubated in 0.05% trypsin at 37℃ for 10 min to obtain single cells. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO
GSM5170104