Illumina MiSeq paired end sequencing
Construction of a mixed viral metagenomic dataset using 25 DNA and RNA viruses
The RNA library was prepared using a combination of the PureLink viral RNA/DNA kit (Life Technologies - Invitrogen) and Zymo RNA clean and concentrator kit, followed by turbo Dnase treatment (Life Technologies – Ambion). Reverse transcription and amplification was performed using the Ovation RNA-Seq version 2 system (Nugen). Libraries were generated using Nextera XT library preparation kit (Illumina).
PUBLIC HEALTH ENGLAND
ena-EXPERIMENT-PUBLIC HEALTH ENGLAND-04-08-2015-13:17:24:219-1