GSM2102460: JEG3_KO_rep3; Homo sapiens; RNA-Seq
JEG3 cells grown to 70% confluence in 10 cm dishes were rinsed once with phosphate-buffered saline (PBS) and harvested with Trizol reagent (Life Technologies). Total RNA was extracted using Trizol, according to manufacturer's instructions and then purified by spin column purification (RNeasy mini kit, Qiagen) using a QIAcube system. RNA was quantified using a Nanodrop (Thermo Fisher) and its integrity assessed on a Bioanalyzer (Agilent) using the RNA 6000 RNA chip. 500 ng high-quality total RNA (RNA Integrity Number >=8) was used as input for TruSeq library construction using the TruSeq RNA Sample Preparation Kit (Illumina). Library purity, correct fragment size, and concentration were assessed using the Bioanalyzer DNA7500 chip. Libraries free of adapter dimers and with a peak region area (220-500 bp) >=80% of the total area were individually barcoded, pooled, and sequenced on an Illumina HiSeq 2000 platform.
GEO
GSM2102460