t6A essentiality
Sequencing of genomic DNA from S. mutans and E. coli was performed by Beckman Genomics (Danvers, MA). Libraries were constructed using the Illumina TruSeq Paired End adaptors and Beckman Coulter SPRI works Library Construction using SPRI-TE instrumentation. All libraries passed QC for library size and concentration (>2nM). Libraries were sequenced on an Illumina HiSeq 2500 instrument at 2 x 100 bp using V4 chemistry. Quality of the sequencing run was determined using FastQC (www.bioinformatics.babraham.ac.uk/projects/fastqc/). Reads were processed using is Illumina’s bcl2fastq version 1.8.4 and fastq_illumina_filter-0.1. Fastq format validation and statistic reporting was done by the fastq_illumina_filter. Read alignment and SNP calling were performed using CLC Genomics Workbench 6.5 (http://www.clcbio.com) using the default settings
University of Florida
E coli B