rad-seq data for longipalpis permethrin survivor # 8
DNA was digested with the restriction enzymes EcoRI and MseI (NEB, Inc.) and adaptor oligonucleotides were ligated onto the digested DNA fragments. The adaptor oligonucleotides included an Illumina adaptor and unique 810 bp identification sequences or barcodes for individual sand fly recognition. Fragment libraries were PCRamplified, pooled together, and fragments between 200 and 300 bp were selected and purified using a Blue Pippin (Sage Science) at the USU Center for Integrated Biosystems (Logan, UT, USA). All DNA libraries (n = 4) were sequenced at the University of Texas Genomic Sequencing and Analysis Facility (Austin, TX, USA).
SUB2918236
LPA8 ; index_10nt_336