GSM2802535: wt_B_polyA; Arabidopsis thaliana; RNA-Seq
Total RNA was extracted with Trizol and treated with RQ1 DNase (Promega) to remove DNA Total RNA was partially fragmented with RNase T1. Polyadenylated mRNAs were purified and concentrated with oligo(dT)-conjugated magnetic beads. 3’ adaptor was added to purified mRNAs, followed by reverse transcription performed with RT primer harboring 5' adaptor sequence and randomized hexamer. The cDNAs were purified and PCR amplified for library construction for Illumina NextSeq 500 system.
GEO
GSM2802535