GSM5342815: scAC: Patient 27 scATAC-seq; Homo sapiens; ATAC-seq
Frozen coronary artery segments were broken down using mortar and pestle under dry ice and liquid nitrogen. Nuclei were isolated using iodixanol/sucrose gradients following the Omni-ATAC protocol (Corces et al. Nature Methods 2017). Nuclei were washed and resuspended in 1X concentration Nuclei Buffer for single cell ATAC sequencing Libraries were constructed according to the 10x Genomics Chromium Single Cell ATAC Reagent Kit protocol (v1 and v1.1 (Next GEM)). Each GEM (gel bead in emulsion) contains a unique 16 nucleotide 10x Barcode sequence. The sample index PCR step during library construction adds a P7 sequence and sample index barcode to each scATAC library. The amplified scATAC libaries contain the 16 nt 10x cell barcode, 8 nt sample index barcode, and P5 and P7 sequences necessary for Illumina sequencing bridge amplification. All protocols to generate scATAC-seq data on the 10x Chromium platform, including sample prep, library prep, instrument and sequencing settings, are available here: https://support.10xgenomics.com/single-cell-atac.
GEO
GSM5342815