night_magna_8
The Qiagen DNEasy Blood & Tissue Kit was used on a sample of 5 Daphnia magna to extract DNA. Samples were taken midday or mid-night to illuminate differences in microbiome composition across circadian rhythms. Libraries were prepared using the 515f/806r universal 16S primer pair, amplifying the V4 region of the 16S rRNA gene. Samples were multiplexed for the Illumina MiSeq and run with the MiSeq v2 Reagent Kit.
SUB9232129
N8