GSM4815626: N2A_GFP-1; Mus musculus; RNA-Seq
Total RNA was isolated from mouse Neuro2A Prox1 and GFP overexpression cells (GFP-Tet-On and Prox1-Tet-On Neuro2A cells) using Trizol (ThermoFisher Scientific). The quantity and quality of RNA samples were analyzed using Agilent RNA 6000 Nano kit with the bioanalyzer from Agilent. RNA samples with RNA Integrity Number (RIN) > 7 were used for library construction using the 3′mRNA-Seq Library Prep Kit Protocol for Ion Torrent (QuantSeq-LEXOGEN™) according to manufacturer's instructions. DNA High Sensitivity Kit was used with the bioanalyzer to assess the quantity and quality of libraries. Libraries were then pooled and templated using the Ion PI™ HiQ OT2 200 Kit (ThermoFisher Scientific) on Ion One Touch System. Sequencing was performed in BSRC 'Alexander Fleming' Genomics Facility using the Ion PI™ HiQ Sequencing 200 Kit and Ion Proton PI™ V2 chips (ThermoFisher Scientific) on an Ion ProtonTM System, according to the manufacturer's instructions.
GEO
GSM4815626