GSM3291135: MTB4; Mus musculus; RNA-Seq
Lungs from mice (infected or uninfected controls) were digested and processed into single cell suspensions. CD8+ T cells were purified from these suspensions using magnetic beads. RNA extraction on these purified populations was performed immediately after. RNA was stored at -80 degrees until it was sent for sequencing. RNA extractions were performed on 5 mice per group, and three samples per group with the highest quality RNA were selected for sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO
GSM3291135