GSM5166270: PI-IBS PI-IBS077; Homo sapiens; RNA-Seq
Colonic biopsies were collected from volunteers and flash frozen using liquid nitrogen. RNA was extracted using Qiagne, miRNeasy Mini Kit per manufacture guidelines. Total RNA was then used using Illumina TruSeq RNA to build sequencing libraries. Created unique dual-indexed (UDI) TruSeq stranded mRNA libraries. All libraries pooled and sequenced on a NovaSeq 2x150-bp Charter-Service RNA-Seq run, generating ≥20 Million reads for each library with mean quality scores for all libraries ≥Q30.
GEO
GSM5166270