GSM1847236: MCF7_Akti1/2_Replicate2 (Cellular small RNA-Seq); Homo sapiens; ncRNA-Seq
Total RNA was purified using RNAeasy miniprep kit (Qiagen) and the RNA integrity was checked using RNA 6000 Nano assay on Agilent 2100 Bioanalyzer. 1µg of total RNA were used for library construction. The library of cellular small RNA was made using Illumina's Tru-Seq Small RNA kit. The 3' and 5' adaptors were ligated and an RT reaction is used to create single stranded cDNA, then PCR amplified using a common primer and one index sequences before size selection on 6% native polyacrylamide gel. Fragment range of 105-150bp corresponding to the small RNA population were excised, eluted, precipitated and resuspended in 20μl of nuclease free water. The size, quality and quantity of the DNA in each final small RNA library were verified using the High Sensitivity kit (Agilent).
GEO
GSM1847236