Transcriptome sequencing of AMF-inoculated Cucumber roots under low temperature stress
The total amount of 20 ?g per sample was used for RNA sample preparations. The enrichment of RNA in cucumber roots for cucumber and R. irregularis was respectively performed via NEBNext Poly (A) mRNA Magnetic Isolation Module (NEB, E7490) and MICROBExpres Bacterial mRNA Enrichment Kit (Invitrogen, AM1905). Constructions of cDNA library were performed by standard Illumina sample preparation protocol using NEBNext mRNA Library Prep Master Mix Set for Illumina (NEB, E6110) and NEBNext Multiplex Oligos for Illuminsa (NEB, E7500). Sequencing of the final purified library was performed on Illumina HiSeq 2500 with one lane of 100 bp reads from both ends of the fragments (pared ends) and 180 bp insert distance for assembly.
SUB1409940
Transcriptome 4