16S Metagenomics of great apes:gut microbia
The laboratory received all frozen samples with codes and was blinded about species origin. RNA later tubes containing fecal samples were centrifuged for 30 minutes at 4400 g to separate the different phases (solid, organic and aqueous). DNA was extracted from 0.25g of the stool sample with the QIAamp Powerfecal DNAextraction kit (Qiagen, Hilden, Germany), following manufacturer instructions. Amplification of region V3-V4 of the 16S rRNA gene and library preparation was performed, as described in Illumina protocol for 16S sequencing with primers S-D-Bact-0341-b-S-17 and S-D-Bact-0785-a-A-21 58. Libraries were sequenced on the Illumina MiSeq platform (Illumina, San Diego, California) with a MiSeq Reagent kit V3
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