GSM4933326: dexa 9 [F9]; Mus musculus; miRNA-Seq
Mature sperm was extracted from cauda epididymis and placed in M2 medium. After passage through a filter, sperm was subjected to somatic lysis and 2 washes. RNA was extracted using Directzol (Zymo R2080). Nextflex small RNA library preparation using indexed barcodes and 1:4 diluted adapters, 22 PCR cycles, starting amount: 100ng RNA.
GEO
GSM4933326