GSM5176644: NICD_TCFWT_ATAC-seq_rep3; Mus musculus; ATAC-seq
50'000 LSK cells were isolated from induced bone marrow and processed as in Current Protocol in Molecular Biology by Buenrostro et al. in 2015 ATAC-Seq: A Method for Assaying Chromatin Accessibility Genome-Wide. Library was amplify using NEBNext High-Fidelity 2X PCR Master Mix and size selection was performed using Agencourt AMPure XP beads. sequencing protocol: Prepared libraries were sequenced on the Illumina NextSeq 500 with paired-end 2x75nt reads to obtain at least 35 million reads per sample.
GEO
GSM5176644