GSM2976849: Sample_69357; Mus musculus; RNA-Seq
RNA was isolated using the RNeasy Plus Micro Kit (Qiagen; 74004) with on column DNasing and assessed for quantity and quality using the Pico or Nano Chip (Agilent). Total RNA was first amplified using the SMARTer technology (SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing; Clonetech 634888 and 634889) with cDNA as the output. The cDNA was input into the Low Input DNA Library Prep Kits for Illumina (Clonetech 634899) it was end repaired A tailed and Illumina adapters were ligated. The resultant library was PCR amplified to generate sufficient material for sequencing. Libraries were clustered on the cBot (illumina) and sequenced on 4 lanes of a 50-cycle paired end HiSeq 4000. HiSeq Control Software version 3.3.52 was used according to manufacturer's protocols. Demultiplexing and Fastq file generation was done using bcl2fastq version 2.17.1.14.
GEO
GSM2976849