GSM2199848: mRNA-RORa KO_CD_2 liver; Mus musculus; RNA-Seq
Total RNA extraction was performed using Trizol (Invitrogen). RNA-seq library was generated using the Illumina TruSeq method according to the manufacturer’s instructions. Libraries were prepared for multiplex sequencing using Illumina TruSeq RNA sample prep kit v2, according to the manufacturer’s protocol. TruSeq universal adapter sequence is 5’-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3'. TruSeq adapter sequences for each sample are following: 1) WT_CD_1: 5’- GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTG-3'; 2) WT_CD_2: 5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTG-3'; 3) WT_HFD_1: 5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTG-3'; 4) WT_HFD_2: 5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTG-3'; 5) KO_CD_1: 5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCCGTCTTCTGCTTG-3'; 6) KO_CD_2: 5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCCGTCTTCTGCTTG-3'; 7) KO_HFD_1: 5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTG-3'; 8) KO_HFD_2: 5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTG-3'
GEO
GSM2199848