Whole genome bisulfite sequencing of Arabidopsis thaliana : 8 day old whole seedling
Approximate 400 ng of Arabidopsis genomic DNA was processed for bisulfite sequencing library construction using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) along with methylated and indexed NEXTflex Bisulfite-Seq Barcodes (bioo scientific). Bisulfite conversion was done using EZ DNA Methylation-Gold Kit (Zymo Research). Sequencing was done on HiSeq3000 platform using 2  101 cycle multiplex paired-end reads.
SUB8624355
WGBS_Cold_22C_100U_GCmethyl_Rep2