pachytene spermatocytes, RNA-seq
Pachytene spermatocytes and round spermatids were isolated from whole testes in adult CD1 wild type male mice by sedimentation at unit gravity using the STA-PUT apparatus.  RNA was extracted using Trizol reagent. Sequencing libraries were prepared using the Illumina RNA-Seq Sample Prep Kit according to the manufacturer’s instructions.
Whitehead Institute for Biomedical Research
pachy_RNA