10X chromium DNA library sequencing on Northern pike
A 10X chromium library was prepared from liver tissue from a single female northern pike individual from the Columbia River around Castlegar, BC - this population is invasive in the region. DNA was extracted by liquid nitrogen grinding, phenol/chloroform/isoamyl alcohol (25:24:1, 3X), and finally purified with Spectrum labs Float-a-lyzer G2 columns (1000 kD - Pink). DNA was sent to McGill University and Genome Quebec Innovation Centre for 10X library construction (multiplex SI-GA-C8) and sequencing on an Illumina HiSeq X instrument with PE 150bp reads.
SUB4306661
Esox_lucius_10X_chromium_March2018