GSM5031935: M28_1: Memory Day 28 rep1 RNA-seq; Mus musculus; RNA-Seq
Spleens from infected mice were recovered 28 dpi, crushed through a nylon mesh and treated with Gey's solution red blood lysis buffer. The 2W1S-specific memory T cell pool was enriched by magnetic enrichment before purfying CD44hi 2W1S-specific CD4 T cells RNA was extracted using the RNeasy Plus Micro Kit (Qiagen) Libraries were constructed using the Smart-SeqTM v4 UltraTM Low Input RNA Kit for Sequencing (Clontech) and the Nextera XT library prep kit and the Nextera XT index kit (Illumina)
GEO
GSM5031935