GSM3897450: RNA-seq 3bKO 1; Mus musculus; RNA-Seq
For RRBS, genomic DNA was isolated from two independent Dnmt3b+/+, Dnmt3b-/- and Dnmt3bCI/CI embryos and tumor cell line using phenol/chloroform. For RNA-seq, total RNA was isolated by trizol from three independent  Dnmt3b+/+; Dnmt3b-/- and Dnmt3bCI/CI embryos. The RRBS libraries were prepared and sequenced on an Illumina HiSeq2500 sequencer. RNA-seq was performed  on NovaSeq 6000 platform using paired-end 150bp runs.
GEO
GSM3897450