GSM4593184: RNA_NPC_Day6_CBX7_KO_Rep2; Mus musculus; RNA-Seq
RNA was isolated from pelleted cells using the PureLink RNA Mini Kit (Invitrogen) according to manufacturer's instructions. Samples were treated with DNase using the TURBO DNA-free Kit (Invitrogen). rRNA was depleted using the NEBNext rRNA Depletion Kit, and cDNA was generated using the NEBNext Ultra II Directional RNA First and Second Strand Synthesis Modules (New England Biolabs). Libraries were prepared as described in Bowman et al., 2013 (BMC Genomics). In brief, this protocol includes end repair, A-tailing, adapter ligation, and real-time PCR amplification, with SPRI cleanup of samples between each step.
GEO
GSM4593184