GSM4933231: G4_S7; Homo sapiens; RNA-Seq
Cellular pellets were resuspended with TRIzol (Thermo Fisher cat. #15596026Ambion #15596018), and total RNA was extracted according to the manufacturer's instructions. Contaminating genomic DNA was removed by DNase (Qiagen cat. #79254) digestion. RNA quality and concentration were assed with the 2100 Bioanalyzer (Agilent cat. #G2939BA) and the Qubit fluorometer (Thermo Fisher cat. # Q33226), respectively. Total RNA-seq libraries were prepared using the Illumina TruSeq library kit (20020597) using 150 ng of total RNA. Libraries were pooled together, and sequenced as single reads with the Illumina HiSeq4000.
GEO
GSM4933231