RNA-Seq of anoikis-resistant  CAL27 cells
Total RNA was isolated from the anoikis-resistant CAL27 samples using TRIzol (Invitrogen, USA) following the manufacturer's instructions. The concentration and quality of RNA samples were determined using a Nano Drop 2000 micro-volume spectrophotometer (Thermo Scientific, USA). The library construction and sequencing were performed by the BGI Genomics Institute, Wuhan, China (https://www.bgi.com/). The library preparation was followed by BGIs standard procedure. The libraries were sequenced on the Illumina HiSeq 2000 platform.
SUB4342404
ARS2