GSM1849284: H3K56ac chIP-seq in H4K44->R mutant yeast, rep #2; Saccharomyces cerevisiae; ChIP-Seq
Sporulating cell samples at different time point were cross-linked with 1% formaldehyde at room temperature for 15 minutes immediately after harvesting. Chromatin immunoprecipitation (ChIP) assays were performed as described in (Govin, J., Schug, J., Krishnamoorthy, T., Dorsey, J., Khochbin, S., and Berger, S.L. (2010b). Genome-wide mapping of histone H4 serine-1 phosphorylation during sporulation in Saccharomyces cerevisiae. Nucleic acids research 38, 4599-4606.) and followed by library preparation. ChIP-seq libraries were prepared using NEBNext® ChIP-Seq Library Prep Reagent Set for Illumina® in accordance with the manufacturer’s instructions and subjected to single-end sequencing using an Illumina® NextSeq instrument.
GEO
GSM1849284