GSM4399905: P14_1_mPFC; Mus musculus; RNA-Seq
Total RNA including miRNA fractions were isolated using Qiagen miRNeasy mini kit according to the manufacturer's protocol. RNA integrity was assessed with an Agilent 2100 Bioanalyzer using an Agilent Bioanalyzer 6000 Nano kit. Input of 400ng of total RNA was used as starting material for each sample and libraries were prepared using 99 TruSeq Stranded Total RNA LT with Ribo-Zero Gold Depletion Kit from Illumina. Quantification was performed using KAPA Library Quantification Kit and the average size of 300 bp was determined by using a High Sensitivity DNA Kit. The libraries were pooled and diluted to 13 pM to load on an Illumina HiSeq 2500 Instrument.
GEO
GSM4399905