GSM3290400: 1: input; Mus musculus; ChIP-Seq
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. a) End repair, Adaptor ligation: The ends of the IP / Input DNA are complemented and phosphorylated at the 5 'end, the 3' end is protruded a sticky end of "A" and then a bubbly joint with a "T" protruding at the 3 'end. b) PCR amplification: The ligation product is subjected to PCR amplification by specific primers. c) Single chain separation and cyclization: PCR products are heat-denatured into single-stranded, and then single-stranded DNA is cyclized with a bridge primer to obtain a single-stranded circular DNA library. d) Sequencing: Sequencing is performed using the resulting amplified product DNA Nano balls (DNB).
GEO
GSM3290400