GSM3453128: ATAC-seq.Sorted-plus2DG_rep2; Mus musculus; ATAC-seq
ATAC-Seq: 2-DG treated and Dox-induced mESCs were FACs sorted to enrich for both GFP- positive and -negative populations. Collected mESCs were lyzed in hypotonic buffer containing NP-40. Isolated cell nuclei were then subjected tagmentation with Illumina Tn5 transposase before PCR amplification with NEBNext 2X PCR mastermix and Illumina Nextera indexed primers.
GEO
GSM3453128