GSM5456588: healthy donor - adipose stromal cells, Rep 4; Homo sapiens; RNA-Seq
Lipoaspiration from 4 healthy donors were collected and enzymatically dissociated. After depletion of hematopoietic cells (Dynal, Dynabeads CD45) stromal cells were sorted by FACS based on their phenotype and RNA extraction (Macherey-Nagel, NucleoSpin RNA XS) was performed. Quality control was performed using the Agilent RNA 6000 Pico Kit. RNA libraries were prepared (with 10ng of total RNA) for sequencing using standard Illumina protocols.
GEO
GSM5456588