RNA-Seq of Parasa lepida: caterpillar urticating hair
Total RNA were isolated from urticating hairs (100 mg) of the final instar caterpillar using GENEzolTM reagent (Geneaid). The cDNA library preparation and sequencing were done by BGI TECH SOLUTIONS (Hongkong). In brief, RNA quantity and quality was assesed using Agilent 2100 Bioanalyzer (Agilent RNA 6000 Nano Kit). The mRNA were isolated from total RNA with oligo(dT) method. The first strand cDNA and second strand cDNA were synthezied and then ligated with adapters. The cDNA fragments with suitable size are selected for the PCR amplification. Sequencing was done on an Illumina HiSeqTM 4000(paired-ended reads, 2 x 100 bp).
SUB8927659
PlepToxin