RRBS analysis of LSK cells obtained from WT mice
DNA extracted from LSK cells of WT mice were first digestedwith MspI restriction enzyme, followed by the ligation with methylatedadaptors at the both end of DNA fragments.DNA fragments of 150-250bp in length were then subjected to bisulfite conversion and amplified by PCR. Sequence was performed at 36bp single end protocol under illumina platform.
HIROSHIMA
DRX016195