GSM1572835: S2 repB_1hr; Drosophila melanogaster; MNase-Seq
MNase-digested DNA samples were resolved on 2% agarose gel. DNA band containing mononucleosomal DNA (<=200bps) was excised and purified by the freeze-squeeze method. Libraries of mononucleosomal DNA were prepared using NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (NEB #E7370S/L), starting with 30ng of mononucleosomal DNA for each reaction. The samples were end-prepared, adaptor-ligated and indexed (NEB #E7335S/L, NEB #E7500S/L) according to the manufacturer’s instructions, followed by purification with AMPure XP beads (Beckman Coulter, Inc. #A63881) without size selection. The quantity and quality of the library were checked with Qubit 2.0 Fluorometer (Life Sciences) and Agilent 2100 Bioanalyzer (Agilent Technologies). Libraries were quantified using KAPA Library Quantification (KK824).  Resulting libraries were plated using the Illumina cBot and run on the Illumina HiSeq 2500 platform configured for 50 bp paired-end reads.
GEO
GSM1572835