Illumina HiSeq 3000 paired end sequencing; ATAC-seq of CP-A Barrett's oesophagus cells
ATAC-seq of CP-A Barrett's oesophagus cells
CP-A cells were trypsinised and collected.  50,000 cells were lysed in ATAC lysis buffer and Tn5 reactions were performed as described in Transposition of native chromatin for fast and sensitive epigenomic profiling of open chromatin, DNA-binding proteins and nucleosome position. 2013. Jason D. Buenrostro, Paul G. Giresi, Lisa C. Zaba, Howard Y. Chang and William J. Greenleaf. doi:10.1038/nmeth.2688  Libraries were constructed by amplifying transposed DNA with Illumina Nextera adapters
UNIVERSITY OF MANCHESTER
E-MTAB-8994:CP-A_p