Minion WGS of Bacillus subtilis phage SPbeta
Bacterial genomic DNA was isolated using EURex Bacterial and Yeast Genomic DNA Kit, while phage DNA was extracted using the phenol:chloroform method as described previously68. The qualities of the extracted gDNA were evaluated by NanoDrop DS-11+ Spectrophotometer (Saveen Werner, Sweden) and electrophoresis, then quantified with the High Sensitivity DNA kit and Qubit 2.0 Fluorometer (Thermo Fisher Scientific). For multiplex MiniON sequencing, the Rapid Barcoding gDNA Sequencing kit was used to allow pooling of samples on a single MinION Flow Cell (FLO-MINSP6). Genomic DNA library was prepared using the SQK-RBK004 rapid barcoding kit and subsequently loaded into the MinION Flow Cell. The local basecalling was performed automatically in real-time using the MinKNOW ONT software (v3.1.19).
SUB8237787
Bacillus subtilis phage SPbeta