GSM4257499: HFD_1; Mus musculus; RNA-Seq
Single cells were extracted from suspensions of lamina propria of each individual mouse replicate. Cells were sorted by flow cytometry (using a FACSAria III, BD Biosciences) and gated for live CD45+Lin-CD11b+CD24-, and Ly6c+ or Cd64+ expression. Sorted cell suspensions from each mouse were loaded into a single well of a 10× Genomics Chromium Single Cell Controller. Single-cell capture and cDNA and library preparation were performed with a Single Cell 3' v2 Reagent Kit (10× Genomics) according to manufacturer's instructions.
GEO
GSM4257499