16S amplicon metagenome of deep sea sediment obtained from off Barren Island, India
The amplified 16S rRNA region was gel eluted using Qiagenminielute columns.50ng of qubit quantified gel eluted sample was used for next cycle of PCR. Index barcodes were added using modified primers which had adapter sequences. 6 cycles of PCR was performed and the product was cleaned up using AgencourtAmpure XP SPRI beads (Beckman Coulter). The prepared library was quantified using Nanodrop and validated for quality by running an aliquot on High Sensitivity Bioanalyzer Chip (Agilent).
National Institute of Ocean Technology
NIOT_BAR_001