GSM4720595: G1 MethylC-seq; Arabidopsis thaliana; Bisulfite-Seq
Galls and control roots were collected at 14 days post infection and frozen on liquid nitrogen. Total RNA was extracted using the AllPrep DNA/RNA/miRNA Universal Kit from Qiagen with several adaptations (Silva et al., 2019; doi: 10.3389/fpls.2019.00657) The libraries were prepared using the Accel-NGS Methyl-Seq DNA Library Kit EZ DNA Methylation-Gold Kit (Zymo Research) following the library protocol Accel-NGS Methyl-Seq DNA Library Kit for Illumina Platforms. The fragment size distribution of the libraries were checked in an Agilent® 2100 Bioanalyzer using DNA 1000 chip (Agilent® Technologies, Inc., Santa Clara, CA, USA). The libraries were quantified using using qPCR according to the Illumina qPCR Quantification Protocol Guide (Bioanalyzer DNA High Sensitivity chip & LightCycle qPCR). The libraries were sequenced using an Illumina® HiSeq X PE150 (San Diego, California, USA).
GEO
GSM4720595