Illumina HiSeq 2500 sequencing; mRNA-seq of Arabidopsis mutants : pol2a-12, cmt3-11, pol2a-12 cmt3-11, fas2-4, pol2a-12 atxr5/6
mRNA-seq of Arabidopsis mutants : pol2a-12, cmt3-11, pol2a-12 cmt3-11, fas2-4, pol2a-12 atxr5/6
13-day-old rosette were cut out and flash frozen in liquid nitrogen Plants were grown in soil in a growth cabinet at 23°C, 50% humidity, using long day conditions (16h light, 8h dark). Total RNA was extracted in TRIzol reagent, precipitated with isopropanol and washed two times in ethanol 70%. RNA was then DNase treated using 1 unit of RQ1 DNAse (Promega) per μg of RNA, following manufacturer's instructions. Following DNase treatment, RNAs were further purified in phenol-chloroform. RNA integrity was assessed by running 1ug of RNA through a agarose gel supplemented with formaldehyde after denaturation in 1X MOPS 4% formaldehyde for 15 minutes at 65°C. Sequencing libraries were generated at Fasteris S.A. (Geneva, Switzerland).
GReD - CNRS UMR 6293 - Inserm U1103
E-MTAB-8939_2:Col-0_rep1_s