GSM2199309: WT_4tUseq; Saccharomyces cerevisiae; RNA-Seq
CLIP and ChIP: Cells were lysed by bead beating and the lysate was sonicated in a Covaris S220 system. Immunoprecipitation was performed at 4 °C with rabbit IgG-conjugated Protein G magnetic Dynabeads. 4tU-seq: Total RNA was extracted and labeled RNA purified. 100 ng of labeled RNA was used as input for the Ovation Universal RNA-Seq System Library Kit (NuGen) and processed following the manufacturer’s protocol. ChIP-seq: Purified DNA from IP and input samples was RNase digested and used for ChIP-seq library perparation with the NEB-Next ChIP-seq Library Reagent Set for Illumina Sequencing (NEB) in combination with Multiplex Oligos (NEB) according to the manufacturers protocol.
GEO
GSM2199309