RNA-Seq of  Acropora digitifera: Post_Spawning
Total RNA from the coral gamete was isolated by homogenizing 100 mg coral tissue in 1 ml TRIzol (Invitrogen) according to the manufacturers instructions. The RNA was then extracted once with 1 volume chloroform and precipitated in ˝ volume isopropanol, washed in 1 volume of 75% ethanol and subsequently dissolved in RNase-free water. These samples were then processed through a 5 M LiCl precipitation overnight at -20? C, washed 3 times with 75% ethanol and subsequently dissolved in RNase-free water. The integrity and quality of the total RNA was assessed using a Bioanalyzer (Agilent Technology). Only the samples showing intact RNA (RNA Integrity number >8) were used for the RNA-seq analysis. The Illumina TruSeq protocol was used to prepare libraries from the RNA samples. This was sequenced using Illumina HiSeq2000 machine.
SUB1410850
Y4