Transcriptome of Anopheles gambiae female embryos
Embryos collected 4-6 and 18-21 hours after oviposition were sexed using PCR assay and RNA samples from each embryo stored. RNA from batches of same sex embryos was extracted using TRIzol protocol. Following RNA treatment with TURBO DNase and evaluation of RNA integrity using Bioanalyzer, polyA  RNA was isolated using Oligotex mRNA mini kit. cDNA was synthesized using SMARTer cDNA synthesis kit and amplified using Advantage 2 PCR kit. Approximately 1 microgram of cDNA was used for construction of sequencing libraries. After cDNA fragmentation libraries were prepared using Rioche 454 Life Sciences kit.
Liverpool School of Tropical Medicine
Agam_Fembr_transcr