GSM2100968: day 16 Aspen (sawdust) replicate 1; Dichomitus squalens; RNA-Seq
Total RNA was extracted from mycelia ground in liquid N2 with the N-cetyl-N,N,N-trimethylammonium bromide (CTAB, Sigma, Germany) method (Chang et al., 1993) and further purified with the RNeasy Plant Mini kit (Qiagen, Germany). Quantity and integrity of RNA were determined spectrophotometrically (NanoDrop ND-1000, NanoDrop Technologies Inc., USA) and with RNA6000 Nano Assay (Agilent 2100 Bioanalyzer, Agilent Technologies, USA). Purification of mRNA, synthesis of cDNA library and sequencing reactions were conducted in the BGI Tech Solutions Co., Ltd. (Hong Kong) as described previously (Patyshakuliyeva et al., 2015) RNA libraries were prepared for sequencing using standard Illumina protocols
GEO
GSM2100968