GSM1847166: control rep1; Mus musculus; RNA-Seq
mES cells cultured in 2i+LIF were collected.Total RNA was isolated using ISOGEN II reagent (NIPPON GENE), according to the manufacturer’s protocol. cDNA was created from 0.5 μg total RNA using an oligo(dT) primer (Life Technologies), random hexamers (TAKARA), and M-MuLV reverse transcriptase (NEB). RNA-seq libraries were prepared using the TruSeq Stranded mRNA LT Sample Prep kit (Illumina)
GEO
GSM1847166