Whole genome resquencing of adaptively evolved end-point population : Escherichia coli K12 MG1655  del crp mutant
The  del crp mutant was evolved under laboratory conditions at 37C in M9 minimal media with glucose as the carbon source and 40 mM MOPS . Passages were done strictly in the exponential phase. Genomic DNA was extracted from end-point populations  using Sigma GenElute Bacterial Genomic DNA Kit (Cat.No. NA2120) following manufacturers protocol. DNA libraries were prepared using the Illumina  TruSeq DNA Nano Kit. Sequencing was done for using 2x100 bp paired-end on a Illumina Hiseq 2500 platform.
SUB7557443
Evocrp1