GSM1847264: HCT116_Akti1/2_Replicate2 (RNA-Seq); Homo sapiens; RNA-Seq
Total RNA was purified using RNAeasy miniprep kit (Qiagen) and the RNA integrity was checked using RNA 6000 Nano assay on Agilent 2100 Bioanalyzer. 4µg of total RNA were used for library construction. Purification, fragmentation and first strand synthesis were performed as described in the Illumina Tru-Seq RNA Sample kit v2. The second strand cDNA synthesis was modified using the dUTP second strand method. End repair, 3' adenylation and adapter ligation steps were done using Tru-Seq protocol.
GEO
GSM1847264