GSM2630323: mouse2_input; Mus musculus; RIP-Seq
For m6A profiling of mouse developing brain, forebrains from wild type E13.5 embryos were dissected and total RNA was extracted from them. mRNA was then isolated using the Dynabeads mRNA Purification Kit (Ambion). m6A pull-down was performed using a rabbit polyclonal anti-m6A antibody (Synaptic systems), and immunoprecipitation with protein G dynabeads (Novex). For m6A profiling of 47 day organoids, the same procedure was followed. cDNA libraries from pulled-down RNA and input RNA were prepared using the NEBNext® Ultra™ DNA Library Prep Kit for Illumina®.
GEO
GSM2630323