16S rRNA gene amplicon sequencing of freshwater sediment metagenome culture: Abiotic + CH4
Microbial 16S rRNA genes were amplified using primers 515F and 806R targeting the V4 region. Nextera 2 nd step PCR incl. pooling. Sequencing on Illumina MiSeq, v2, 2x250 bp.
SUB9404058
AP8