Rna-seq of Poly-A+ mRNA from C. elegans strain SP1560, mec-8(u218) : whole animal, L4 larval stage
C. elegans worms were synchronized by bleaching and grown to larval stage L4 under standard laboratory culturing conditions, NGM media with OP50 food source. Total RNA was extracted by Trizol as described in PMID:19778439 and PMID:24910101. poly-A RNA was purified and Library preparation was performed by the Illumina Fastback Genetic Analysis Services in Hayward, CA.
SUB3103908
SP1560_mec-8_L4_polyA