GSM2803526: meis2b-_1; Danio rerio; RNA-Seq
The hearts were extracted and the atria dissected into the meis2b-positive and meis2b-negative halves.  RNA was isolated using the miRNeasy micro Kit (Qiagen) combined with on-column DNase digestion (DNase-Free DNase Set, Qiagen) to avoid contamination by genomic DNA. Truseq Stranded mRNA Kit. Briefly, 50-100ng of RNA were used as input for Library preparation following the low sample protocol (Illumina). Sequencing was performed on the NextSeq500 instrument (Illumina) using v2 chemistry, resulting in minimum of 27M reads per library with 2x75bp paired end setup.
GEO
GSM2803526