GSM2101551: capture in-situ Hi-C; Homo sapiens; OTHER
In situ Hi-C was performed as per Rao et al (2014) using MboI to digest chromatin. Four PCR reactions of 9 cycles each were performed and an Illumina sequencing library was prepared on the material. For the capture step, the Hi-C library was hybridyzed to 81,735 biotinylated 120 bp custom RNA probes purchased from Custom Arrays designed on 4 nearest MboI restriction ends near human RefSeq promoters. Twelve cycles of PCR were performed on the captured Hi-C library for sequencing. We used the standard Illumina protocol for immobilized HiC libraries
GEO
GSM2101551