Tag-Seq library with RNA-RNA P5 adaptor ligation, polyT-bead selection and polyT-primer reverse transcription. The samples were labelled according to Illumina's double-indexing system with P5 adaptors harboring moderately degenerate regions. Eight libraries from six biological samples of Daphnia pulex R9 were labeled as follows:Clutch Treatment i7_seq i5_seq Inline adaptor bases ExFold spike-in mixjI30c2 (1) Control ATTCAGAA NNYRYYRR NNN 2jI30c2 (2) Control GAATTCGT NNYRYYRR NN 1jI30c3 Control CTGAAGCT NNYRYYRR NN 2jI30c4 Control TAATGCGC NNYYRRYR NN 1jI30n1 Charoborus kairomone CGGCTATG NNYRYYRR NN 2jI30n2 Charoborus kairomone TCCGCGAA NNRYRYRY NN 1jI30n4 (1) Charoborus kairomone TCTCGCGC NNYRYYRR NNN 2jI30n4 (2) Charoborus kairomone AGCGATAG NNYRYYRR NN 1
SUB1416057
Daphnia pulex R9 test Tag-Seq library