Castanea sativa and Castanea crenata Transcriptome or Gene expression
oly(A) RNA was enriched from 15-30 *g of total RNA using two rounds of the MicroPoly(A) Purist Kit (Applied Biosystems, Ambion, CA), according to the manufacturer’s instructions. A fraction of 200 nanograms of Poly(A)  RNA of each isolate was used as starting material for cDNA library construction using Multiplex Identifiers (MIDs) according the cDNA Rapid Library Preparation Method Manual, GS FLX Titanium Series, October 2009 (Roche-454 Life Sciences, Brandford, CT, USA). Briefly, preparing the cDNA libraries consisted of fragmentation of RNA, double-stranded cDNA synthesis using the cDNA Synthesis System Kit with Primer ‘‘random’’ (Roche Diagnostics GmbH, Mannheim, Germany), rapid library MID adaptor ligation and cDNA library quality assessment with a High Sensitivity DNA chip (Agilent Technologies, Palo Alto, CA) on Agilent 2100 Bioanalyzer.
BioFIG
Castanea