GSM5176709: LNCaP_VPRBP Si2_INPUT; Homo sapiens; ChIP-Seq
After 24h nutlin-3a treatment or 3 days post transfection, cells were fixed with 1% formaldehyde and chromatin prepared by sonication in Bioruptor plus. Fragmentation efficiency was analysed using fragment analyser and ChIP carried out using diagenode iDeal ChIP-seq kit for transcription factors (Cat# C01010170). Libraries were prepared using Diagenode MicroPlex Library Preparation Kit v2 (Cat# C05010014) and purified and purified/size selected using Agencourt® AMPure® XP beads (Beckman Coulter, Cat. No. A63881)
GEO
GSM5176709