Comparison on the Triclosan induced Ecotoxicological Responses and the Intestinal Microbial Community in Earthworm Eisenia fetida
Triclosan (TCS) caused effects on ecotoxicological responses and on community of intestinal microorganism in the earthworm E. fetida were examined in a seven-day exposure. TCS concentration in earthworms’ intestines was higher than those in the epidermis, which suggested that earthworms accumulate TCS mainly by ingesting it with soil particles rather than by penetrating it through their epidermis. TCS treatments induced a concentration-dependent increase of antioxidant (superoxide dismutase, SOD; catalase, CAT; peroxidase, POD) and malondialdehyde (MDA) level, with the maximum fold changes observed at the highest tested concentration. Microbial community analysis demonstrated that enhanced TCS exposure caused substantial alteration within the Bacterial and Eukaryota community diversity of earthworm intestine. The sensitive genuses were gradually depressed with the increasing TCS concentration, while Pseudomonas, Stenotrophomonas and Achromobacter genus were notably stimulated by the increase of TCS concentration. Nine susceptible microbial groups were more sensitive than enzyme activities and can be used as pioneer to monitor adverse effects of TCS.
Nanjing University
TCS earthworm