GSM2628184: K4_16S rRNA Seq; Drosophila melanogaster; OTHER
Total genome DNA from 30 adult fly midguts was extracted using the kit (Tiangen, Beijing, China). DNA concentration and purity was assessed on the Qubit@ 2.0 Fluorometer (Thermo Scientific). 16S rRNA genes were amplified used the specific V4 primers 515F-806R. All PCR products and operate electrophoresis on 2% agarose gel for detection. Samples with bright main strip between 400-450bp were chosen for further experiments. Libraries was sequenced on an Illumina HiSe platform and 250 bp paired-end reads were generated.
GEO
GSM2628184