GSM3454224: FF1_CRE_AF; Mus musculus; RNA-Seq
Total RNA was extracted from the indicated cell cultures using PerfectPure RNA cultured cell kit (cat # 2302340, 5 Prime). To avoid DNA contaminations all samples were treated with DNase (5 Prime). RNA integrity was evaluated on Bioanalyzer (Agilent 2100 Bioanalyzer), requiring a minimal RNA integrity number (RIN) of 8.5. Libraries were prepared according to Illumina's instructions accompanying the TruSeq RNA Sample Preparation Kit v2 (Cat # RS-122-2001). Sequencing was carried out on Illumina HiSeq2500 according to the manufacturer's instructions, using 10 pM template per sample for cluster generation, and sequencing kit V2 (Illumina). TruSeq RNA sample preparation Kit v2 protocol (Illumina)
GEO
GSM3454224