GSM3357071: SNU16 Capture-C; Homo sapiens; OTHER
Capture-C probes at CCNE1 promoter regions were designed using CapSequm. Approximately 1 × 10e7 cells were crosslinked by 2% formaldehyde, followed by lysis, homogenization, DpnII digestion, ligation and de-crosslinking. DNA was sonicated using a Covaris to 150–200 bp to produce DNA suitable for oligo capture. Three micrograms of sheared DNA was used for sequencing library preparation (New England Biolabs). The promoter sequence of CCNE1 was double captured by sequential hybridization to customized biotinylated oligos (IDT, Supplementary Table 14) and enrichment with Dynabeads (LifeTech). Captured DNA was sequenced on the Illumina HiSeq 3000 instrument with the 2x150 bp configuration.
GEO
GSM3357071