GSM5169184: C2C12_short_1k; Mus musculus; RNA-Seq
Cells were rinsed with 1X PBS and incubated with 2 mL TrypLE-Express for 5 minutes at 37C, which was then neutralized with 8 mL myoblast growth media. RNA was reverse transcribed in situ and barcoded cDNA was extracted following Parse Biosciences' Single Cell Whole Transcriptome Kit (also called Split-seq). PacBio long-Split-seq: SMRTbell Template Prep Kit (PacBio, 100-938-900) following manufacturer's protocol with 500 ng of amplified, barcoded cDNA. Illumina Split-seq: Parse Biosciences' Single Cell Whole Transcriptome Kit (also called Split-seq). scRNA-seq
GEO
GSM5169184