Exome from  AIRAPLN650  bone marrow polymorphonuclear cells
Genomic DNA from bone marrow polymorphonuclear cells (200 ng) was sheared and used for the construction of a paired-end sequencing library as described in the protocol provided by Illumina. Enrichment of exonic sequences was then performed for each library using the SureSelectXT Mouse All Exon kit (Agilent) and following the manufacturer’s instructions. Exon-enriched DNA was precipitated with magnetic beads coated with streptavidin (Invitrogen) and was washed and eluted. 18 additional cycles of amplification were then performed on the captured library. Enriched libraries were sequenced in two lanes of an Illumina Gene Analyzer II× sequencer, using the standard protocol. Data analysis and identification of sequence variants was performed using the Sidrón pipeline in a HD Genome One Research Edition station (Dreamgenics, Asturias, Spain)
Universidad de Oviedo
AIRAPLN650