multiplex1
DNA extraction was performed on the frozen rotifer biomass using JETFLEX Genomic DNA purification kit (GENOMED, Lhne, Germany) following manufacturers instructions. GBS libraries were prepared and sequenced at the Institute for Genomic Diversity (IGD, Ithaca, NY, USA) following the protocol described by Elshire et al. (2011). In brief: (1) DNA samples were digested with the restriction enzyme ApeKI (GC[A-T]GC), (2) GBS libraries were constructed with unique barcodes for each clone, and (3) after pooling of the samples, sequencing was carried out using an Illumina HiSeq 2000/2500 (100 bp, single-end). A library with unique barcodes for each clone, plus blank samples, was created.  Barcodes sample: CGAA,1_1TAGTGC,1_2bCAGCGTA,1_3cCTGAATT,1_4GTAGAGC,1_5ACGTGTGT,1_6cCGCTTGAA,1_7AACGAGTA,BLANK1TAACGAGA,5_30GTACTC,5_29ATGTCAA,5_28ATAGTCA,5_27ATGATCT,5_26GAAGACAT,5_25bTGGCTATT,5_24CACCACGT,5_23ATACAGGA,6_2ACCTGC,6_4bCACAGGT,6_5TAGCCAT,BLANK2CATGTTA,6_6cTGTCAAGA,6_7cACCACCGT,6_8TCATTGTA,6_9CACAGAGT,BLANK3CTTGAC,6_30GGTGGCA,6_29AGGAGTC,6_28TGTAAGC,6_27GCGAGGTT,6_26CTATGTAA,6_25GGTTCATT,6_24GTCC,1_30cGTTCCA,1_29AGTTCGA,1_28TCGTTAA,1_27GACCTAT,1_26CAAGTGAA,1_25ATCGGTGT,1_24CCGACCAT,BLANK4ACAT,2_1TGAATC,2_2GCTAATT,2_4TGATCAT,2_5CATTAGT,2_6GTTAACGA,2_7CCTACCGA,2_8TGTCTTAA,2_9GCACT,2_30CGCCAT,2_29ACGCTGA,2_28CGTCGGA,2_27ATTAGCA,2_26TCGTGGAT,2_25GGACAATA,2_24GTATACTA,2_23AGCTA,4_3cATCGTC,4_4TAAGCTT,4_5GACTATT,4_8GAATCTA,4_9AGCGATAA,4_10AACGGAAT,4_11TGTGCAGT,4_12CAGCT,4_30GTCACT,4_ACTTAGAT,BLANK5TAGGTCA,4_28TGTCATT,4_27CCACCAGT,4_26TCGATGGT,4_25ATGCTGGT,4_20TATCTGA,3_1CATCGC,3_2TGATACA,3_3GCAACGT,3_4bGCCAGAT,3_5GGCGTGTA,3_6GTTCATTA,3_7GCAAGTAA,3_8TTCAAGT,3_30AGGTCT,3_29ACCGAGT,3_28ACTTGAT,3_27CCAGGTA,3_26AAGACAGT,3_25GAATCGAA,3_24CAGGAATA,3_23GAGCAGT,5_1CCTGCA,5_2GTACTTA,5_3TCTGATA,5_4TGGCAAC,5_5TTGTGCGA,5_6CATGGTGT,5_7GTCTGGAT,5_8AGGC,1_8AGCATT,1_9AATCGTT,1_10TCAAGTT,1_11ACGTGTA,1_12CAAGAAGT,1_13AACGTAGA,1_14AGCTTGGT,1_15ACAATGGA,5_17TGACCT,5_18ATCGTAT,5_19CAGTTGA,5_20CAATGTT,5_21AGTTGGCA,5_22GCGGATGT,BLANK6ACAAGAGT,BLANK7TTGCAC,6_10bTACTGAT,6_11TGTGCAA,6_12GTTACGA,6_13TCGCAAGT,6_14CATTGAGA,6_15TTGGCGGA,6_16GAACATGA,6_17CTAGCT,6_18CTTGAGA,6_19CGACAGT,6_20TGCATAT,6_21CGATGTGT,6_22GTAACAGA,6_23GATT,1_16CTCCGA,1_17CTATGGA,1_18GATCATA,1_19GATGCAT,1_20GTCATGGT,1_21CTCACGGA,1_22ACAGATGA,1_23ACCGT,2_10TTGGCA,2_11TACGGTA,2_12GCATTGA,2_13cCTAATGT,2_14AACAGTGA,2_15CGTCA,2_16CCACGT,2_17ACTATGT,2_18CAGGTAT,2_19GTCGATA,2_20GTGCAAGA,2_21ACGTAAGA,2_22TCGCA,4_13GATGTC,4_14CGTGAAT,4_15TGCAATA,4_16TATACGT,4_17CAATAGGA,4_18CGTATGGT,4_19CGCAT,BLANK8TGTTAC,BLANK9GGAGCTGT,BLANK10TCATAGT,3_9CAGTTA,3_10AACTTGT,3_11AGTCTAT,3_12bAGCGTTA,3_13AGGCTAGA,3_14TTCGAAGA,3_15CTGAGTGT,3_16TTACGAT,3_17GCCTAT,3_18TGACGTA,3_19GTCTGAA,3_20ATCCGGA,3_21CTAGTGGT,3_22GGCTAGA,5_9AGTGGC,5_10GCTATAA,5_11ATCAGTT,5_12TCAGTAT,5_13GCTAGTGT,5_14TGACTGGT,5_15TGCATAGA,5_16
SUB4718854
LIB_1