GSM5170557: EN7-GFP-AGO1-ktn1-r1; Arabidopsis thaliana; RIP-Seq
9-day-old Arabidopsis roots of EN7::GFP-AGO1 and ACL5::GFP-AGO1 transgenic lines in WT and ktn1-20 backgrounds were collected and ground into powder in liquid nitrogen and subjected to immunoprecipitation using GFP-Trap_MA. 14-day-old seedlings were ground to a fine powder in liquid nitrogen and subjected to RNA extraction using TRI Reagent. Small RNA library construction using NEBNext Multiplex Small RNA Library Prep Set for Illumina (E7300).
GEO
GSM5170557