Illumina MiSeq paired end sequencing; SAMHD1 restrains aberrant nucleotide insertions at repair junctions generated by DNA end joining
SAMHD1 restrains aberrant nucleotide insertions at repair junctions generated by DNA end joining
HEK 293 or HeLa cells were seeded and transfected with the indicated constructs for indicated time points.   Genomic DNA was isolated using the DNeasy Blood & Tissue Kit (QIAGEN) according to the manufacturers instructions.  Repaired plasmids and genomic junctions were PCR-amplified using specific primers with different 6bp 5â€™ tags. For genomic amplicons, the Nextera XT Index Kit (Illumina) was used. Amplicons were pooled and sequenced on the Illumina MiSeq platform.
1 Department of Internal Medicine III with Haematology , Medical Oncology, Haemostaseology, Infectiology and Rheumatology, Oncologic Center, Salzburg Cancer Research Institute Laboratory for Immunological and Molecular Cancer Research (SCRI LIMCR), Parace
E-MTAB-83821597143176:NAII_N_2_p