WT Primary Spermatocytes 5' monophosphorylated long RNAs rep1
Total RNA from sorted mouse germ cells was extracted using mirVana miRNA isolation kit (Thermo Fisher, AM1560) and used to prepare a library of 5? monophosphorylated long RNAs as described (Wang et al., 2014). Libraries for two independent biological replicates were sequenced using a NextSeq 500 (Illumina) to obtain 75 + 75 nt, paired-end reads.
SUB4468410
Zamore77