GSM2199537: ko Rep3; Rattus norvegicus; RNA-Seq
Rats were euthanized under anesthesia according to approved IACUC protocols. Hypothalami were rapidly dissected from brain tissue samples on ice and immediately snap frozen in liquid nitrogen. RNA extraction (N=4 biological replicates of each genotype, male Mecp2ZFN/y and male wild-type littermate rats, 6 weeks of life) was performed using TRizol reagent (ThermoFisher Scientific) and further purified with on-column DNase treatment using the RNeasy Mini Kit (Qiagen)  following the manufacturers’ instructions. Ten µg of RNA was prepared for paired-end RNA-Sequencing using Illumina HiSeq 2500. The Baylor College of Medicine Genomic and RNA Profiling Core first conducted Sample Quality checks of the RNA using the NanoDrop ND-1000 spectrophotometer and Agilent Bioanalyzer 2100 using the Nano chip.  The Illumina EpiCentre Ribo-Zero rRNA Removal Kit was used to reduce the ribosomal RNA content.  The rRNA-reduced sample was then carried into the Illumina TruSeq RNA library preparation protocol.
GEO
GSM2199537