GSM3665976: H3S10ph_mitosis_ChIPSeq_Rep1; Mus musculus; ChIP-Seq
Lysates were prepared from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq samples were prepared by end repair, 3' adenylation, and adaptor ligation using Illumina's ChIP-seq Sample Preparation Kit. SPRIselect (Beckman Coulter) beads were used obtain an average library target size of 338 bp (range 302-436 bp), followed by real-time qPCR quantification using the KAPA Library Quant Kit for Illumina (KAPA Biosystems catalog no. KK4835). Single-end sequencing (1x50bp or 1x75 bp) was performed on the Illumina NextSeq 500 in high-output mode using Illumina sequencing reagents according to Illumina instructions.
GEO
GSM3665976