GSM5261769: RD WT rep 3; Homo sapiens; RNA-Seq
RNA was harvested using Trizol reagent. RNA purity and integrity were assessed with Nanodrop and agarose gel electrophoresis and Agilent 2100 respectively. mRNA fromis purified from total RNA using poly-T oligo-attached magnetic beads The mRNA is first fragmented randomly by addition of fragmentation buffer. RNA libraries were prepared for sequencing using NEBNext Ultra RNA Library Prep Kit for Illumina
GEO
GSM5261769