Genomic PCR of exon 2 of zfp36l1 in pSpCas9(BB)-2A-Puro (PX459) V2.0  empty vector transfected U2OS TREx monoclonal cell line
U2OS-TREx cells were cultured in DMEM supplemented with 10% fetal bovine serum (Tet Minus FBS; PanBiotech) and 1% penicillin/streptomycin (Invitrogen). Genomic DNA from pSpCas9(BB)-2A-Puro (PX459) V2.0 - empty vector transfected cells was extracted using Qiagen QiaAmp DNA mini kit (#51304) according to the manufacturers instructions and was used as a template for PCR amplification. Forward (5-CTGCTGGACAGAAAGGCAGT-3) and reverse (5-ATCCACAACGCTGAAGAGCGC-3) primers spanning the CRISPR-edited region  for amplification of a 450bp fragment of Exon2 of zfp36l1
SUB9312300
GenLab-U2OSTREx-ZFP36L1- EV