GSM3290025: LV4 (P7); Mus musculus; RNA-Seq
Total RNA were isolated from all biological samples using TRIzol reagent following the manufacturer's protocol. 4 µg of total RNA was used for library preparation with the KAPA stranded mRNA Library prep kit for Illumina platform (KAPA Biosystems). Library concentrations were quantified with the Qubit dsDNA HS (High Sensitivity) Assay Kit (Invitrogen, Q32854), and equimolar amounts were pooled for single-end sequencing on an Illumina HiSeq 4000 instrument (Illumina) to yield ~20 million reads (range 13–23 million) of 36 bp or 51 bp in length per sample.
GEO
GSM3290025