GSM1575425: Vehicle Rep #3; Mus musculus; RNA-Seq
GIST tumors from the cecums of mice were explanted and grounded in 1000 μl Trizol (Invitrogen) using a PowerGen homogenizer (Fisher Scientific), followed by addition of 200 μl chloroform. The samples were then centrifuged at 10,000g for 15 min. The upper phase was mixed with an equal volume of 70% ethanol, and the RNA was further purified using E.Z.N.A total RNA kit (Omega). RNA libraries were prepared for sequencing using standard Illumina protocols
GEO
GSM1575425