RNA-seq of Lasiodiplodia theobromae after infecting Camellia sinensis leaves
The tea leaves infected by Lasiodiplodia theobromae from 15 plants were pooled together to avoid variations in individual plants. Leaf samples were ground into fine powder under the protection of liquid nitrogen. About 100 mg powder of each tea leaf was used for RNA extraction. Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. We performed the sequencing on Illumina Hiseq 4000 (LC Sceiences,USA) to yield 2 x 150 bp paired-end raw reads following the vendor's recommended protocol.
SUB7543930
Lt3