GSM2801023: mC_K_nitens; Klebsormidium nitens; Bisulfite-Seq
DNeasy Plant Mini kit (Cat. No. 69104, Qiagen) Genomic DNA was fragmented with a Covaris S2 sonicator to a mean length of 200 bp, then end-repaired, A-tailed, ligated to methylated Illumina TruSeq adapters, and subjected to 4 cycles of PCR amplification with KAPA HiFi Uracil+ DNA polymerase (KAPA Biosystems).
GEO
GSM2801023