GSM4257662: TK3_WGS; Homo sapiens; OTHER
To extract genomic DNA, sorted cells were incubated in 400 μl of lysis buffer (50mM Tris with pH 8.0, 100mM NaCl, 25 mM EDTA with pH 8.0, 0.5% SDS, and 0.5 mg/ml Proteinase K) at 65 oC for 2 hrs. Genomic DNA was precipitated by adding 500 μl of isopropanol into cell lysis buffer. After centrifugation, DNA was washed once with 500 μl 70% ethanol and air dried before being re-suspended as template for PCR. The qualified genomic DNA sample was randomly fragmented by Covaris technology and the fragment of 350bp was obtained after fragment selection
GEO
GSM4257662