GSM2801011: DO_FAKi_SSC_1_ATAC-seq; Mus musculus; ATAC-seq
Nuclei were isolated using NP-40, Tween-20, and Digitonin-containing buffer and subsequently transposed with adapter-loaded hyperactive Tn5 transposase (Illumina) in the presence of Tween and Digitonin. Following clean-up transposed fragments were directly amplified and barcoded using Nextera indexes Libraries were then sequenced using Illumina NextSeq 500 with 2x75 bp reads
GEO
GSM2801011