GSM1207795: ESC_Hira_WT_Catch-it; Mus musculus; OTHER
Samples were prepared as previously described (Deal et al, 2010). Briefly, cells were labeled with azidohomoalanine for 30 min. MNase digested chromatin was treated with biotin alkyne, and labeled chromatin was captured using streptavidin beads. Samples were treated with urea to remove non-H3 associated DNA, and remaining DNA was purified for use in library generation. Libraries were constructed according to the Illumina protocol.
GEO
GSM1207795