GSM5343199: Male 3; Taxus x media; RNA-Seq
Fresh twigs were harvested from both of three independent female and male trees. After dried and grounded into fine powder, samples were used to prepare crude extracts according to a classic method RNA extraction was performed using an RNeasy plant mini kit (Qiagen, Hilden,Germany). The mRNAs were purified by oligo (dT) attached magnetic beads and fragmented into small fragments. The fragmented mRNAs were reverse-transcribed to create cDNA libraries using a sample preparation kit (Illumina, San Diego, USA). Sequencing was carried out using an Illumina Hiseq 4000 platform (LC-Bio, Hangzhou, China) to produce raw 100/50-bp paried/single-end reads according to the manufacturer's protocol.
GEO
GSM5343199