GSM3665822: YS-D28 rep2; Sus scrofa; RNA-Seq
For isolation of genomic DNA (gDNA) from cells and tissues, the QIAamp mini DNA Kit (Qiagen) was used according to the manufacturers' instructions. Total RNA was isolated using Trizol plus RNeasy mini kit (Qiagen) and mRNA from individual blastocysts was extracted using the Dynabeads mRNA Direct Kit (Dynal Asa, Oslo, Norway). Synthesis of cDNA was performed using a High Capacity cDNA Reverse transcription kit (Applied Biosystems; ABI, Foster City, CA) according to the manufacturers' instructions. The QIAseq FX Single Cell RNA Library kit (Qiagen) was used for Illumina library preparation and transcriptomics analysis.
GEO
GSM3665822