SLAF-seq of hulless barley: leaf
RsaI and EcoRV-HF (New England biolabs, NEB) were selected for digesting the hulless barley genome DNA. Collected the SLAF tags (364-483bp) and linked to Dual-index sequencing adapters, and then the SLAF libarary was sequencing using an Illumina high-throughput sequencing platform
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