GSM3453536: e13.5control_replicate; Mus musculus; RNA-Seq
Embryonic dorsolateral/flank skin was micro-dissected and pooled (3-4 embryos per sample) and dissociated into a single-cell suspension using 0.25% trypsin (Gibco, Life Technologies) for 30 minutes at 37° C. Single-cell suspensions were then stained with DAPI (Fisher Scientific, NBP2-31156) just prior to fluorescence-activated cell sorting. Chromium Single cell 3' Library and Gel Bead Kit v2 Chromium Single Cell 3′ Library & Gel Bead Kit v2 (PN- 120237), Chromium Single Cell 3′ Chip kit v2 (PN-120236) and Chromium i7 Multiplex Kit (PN-120262) were used according to the manufacturer's instructions in the Chromium Single Cell 3′ Reagents Kits V2 User Guide. After cDNA libraries were created, they were subjected to HiSeq 2500 (Illumina) sequencing.
GEO
GSM3453536