GSM2101051: RNA_diploid_131; Arabidopsis thaliana; RNA-Seq
Total RNA was isolated from rosette leaves using a Plant total RNA Purification kit Cat#TR02 (Gene Mark, Taiwan). The procedure involves cell lysis and protein precipitation followed by centrifugation. RNA in the supernatant is then bound on a filter column, where it is digested with DNaseI, followed by washing, and eluting with nuclease-free waster. RNA was quantified with the Qubit RNA BR Assay and 0.4 μg of purified RNA with ERCC-ExFold mix(diploid with mix1 and others with mix2) spiked-in control was utilized as starting material for library construction, which was carried out with the Illumina TruSeq RNA Sample Preparation kit following the manufacturer's instructions. Libraries were sequenced using 100-bp reads on the HiSeq 2000 System (Illumina).
GEO
GSM2101051