GSM5176585: NICD_TCFKO_3 [RNIC-Tg-3_S6]; Mus musculus; RNA-Seq
Cells were isolated from sorted bone marrow LSK and RNA was harvested using PicoPure™ RNA Isolation Kit (Thermo Fisher Scientific, KIT0204) according to manufacturer's instructions We performed cDNA library with the "Illumina TruSeq RNA Library Prep kit" We then performed a Nextera XT library prep according to Illumina protocol 15031942 Rev. E. We quantified the libraries by qubit DNA HS and performed profile analysis by TapeStation 4200. We performed a single end 75nt sequencings on NextSeq 500.
GEO
GSM5176585