GSM5170566: IL22Fc_gene expression; Mus musculus; RNA-Seq
Single cell suspension from mouse colon was obtained by enzymatic digestion following the Miltenyi Biotec Lamina Propria Dissociation Kit (130-097-410). Cells then were stained with both flow cytometry and CITEseq (HTO and ADTs) antibodies prior sorting to enrich for CD45+ population (80% CD45+B220-, 10% CD45+B220+, 10% CD45-) for each treatment group. Sorted cells then were loaded in the Chromium 10x genomics controller following manufacturer V2 protocol. A single cell library from each experimental group was prepared following the 10x genomics V2 protocol. Material was QC and quantified by TapeStation D5000 (Agilent) prior to sequencing using a NextSeq 500 (Illumina). The final library was a combination of each sublibrary in a proportion 21.5:1.25:2.5 (Transcriptome: HTO: ADT) from each one.
GEO
GSM5170566