Genomic-based identification of environmental and clinical Listeria monocytogenes strains associated with an abortion outbreak in beef heifers
Single Molecule, Real-Time (SMRT) sequencing was performed on the Pacific Biosciences (PacBio) RSII sequencing platform using PacBio SMRTbell Template Prep kit 1.0., with the P6/C4 sequencing chemistry and 360-min data collection protocols. DNA was extracted using 100/G columns (Qiagen), sheared using a Corvaris g-tube, and libraries were prepared from 10 mg of bacterial genomic DNA, using the standard protocol, and processed for sequencing as recommended by the supplier.
SUB4585501
52864-6443