GSM2976788: bESC_B_H3K27me3; Bos taurus; ChIP-Seq
Chromatin from bESCs was prepared following the protocol recommended by the True MicroChIP manual (C01010130; Diagenode). Sonication was performed using a Covaris S220 ultrasonicator (500217; Covaris) with the following settings: duty cycle 5%, intensity 3, cycle/burst 200, number of cycles: 12 (60 s). Sequencing libraries were prepared following the manufacturer's instructions using the ThruPLEX DNA-seq kit (R400406;Rubicon) with 16 cycles in the library-amplification step.
GEO
GSM2976788