Targeted-Capture sequencing of Homo sapiens:C708
"Genomic DNA was diluted and digested using restriction enzymes. DNA fragments were hybridized to HaloPlex probes and sample indexes, captured using streptavidin beads, and ligated into circulated fragments per the manufacturer's protocol. Target libraries were amplified and purified, and indexed samples were pooled for multiplexed sequencing. Sequencing libraries (25 pM) were chemically denatured and applied to an Illumina HiSeq v4 paired end flow cell using an Illumina cBot. Hybridized molecules were clonally amplified and annealed to sequencing primers with reagents from an Illumina HiSeq PE Cluster Kit v4-cBot (PE-401-4001)."
MDACC-phs001751
C708