Buckwheat
Total RNA was isolated from the frozen seedlings of tartary buckwheat using an RNeasy Mini Kit (Qiagen, Valencia, USA) and cleaned by ethanol precipitation. We removed the rRNAs in the total RNA using a ribo-zero rRNA removal kit (Epicentre, RZPL11016) and constructed a cDNA library for RNA sequencing using a TruSeq stranded total RNA sample prep kit-LT set A and B (Illumina, RS-122-2301 and 2302) according to the manufacturer s instructions (Illumina, San Diego, CA, USA). The cDNA library was sequenced in 76 bp length paired-end (PE) reads in an Illumina NextSeq500 sequencer (Illumina Inc., San Diego, CA, USA) to produce 69,570,892 raw sequencing reads.
SUB3606153
T8 0h