BS-Seq of Pyrus x bretschneideri: ovary
Total genomic DNA was extracted from ovary with two biological replicates following previous protocol. By using a sonicator (Covaris S220), 5.2 g genomic DNA and 26ng lambda DNA was fragmented to 200-300bp, end repairing and adenylation was followed. Lambda DNA was used to estimate the bisulfite conversion rate. The DNA fragments were then treated twice using EZ DNA Methylation-GoldTM Kit (Zymo Research). Library concentration was quantified by Qubit 2.0 Flurometer (Life Technologies, CA, USA) and quantitative PCR, and the insert size was assayed on Agilent Bioanalyzer 2100 system. The library preparations were sequenced on an Illumina Hiseq 2500
SUB4723408
Ovary methylation biological replicate 1