WXS of Homo  s.: adult male carotid body tumor
DNA was extracted from blood cells using a MagNA Pure Compact Nucleic Acid Isolation Kit I (Roche, Switzerland) on a MagNA Pure Compact Instrument (Roche); DNA from tumour and lymph node tissues was isolated with High Pure FFPET DNA Isolation Kit (Roche). Briefly, DNA (100 ng per sample) was sheared to 150 bp using Covaris S 220 System (Thermo Fisher Scientific, USA) and was then subjected to library preparation with TruSeq Exome Library Prep Kit (Illumina, USA) according to manufacturer instructions. The exome libraries were sequenced on a NextSeq 500 System (Illumina) with paired-end reads, 76 bp (for tumor and lymph node) and 151bp (for blood) in lenght at 300x minimal coverage for tumor and lymph node samples and 200x coverage for blood samples.
SUB4176034
11t