GSM4933311: dexa 1 week exposed sperm collected in 3 ml only 2 ml taken and only cauda  [KG10.7]; Mus musculus; miRNA-Seq
Mature sperm was extracted from cauda epididymis and placed in M2 medium. Sperm was subjected to somatic lysis and 2 washes. RNA was extracted using Trizol. TruSeq small RNA library preparation using 1:4 diluted adapters, 22 PCR cycles, starting amount: 100ng RNA.
GEO
GSM4933311