Illumina HiSeq 1500 paired end sequencing; Affect of antiviral ribavirin on YFV WT and vaccine strains.
Affect of antiviral ribavirin on YFV WT and vaccine strains.
Virus was collected from Ribavirin treated cell supernatant. Supernatant was collected and centrifuged at 1500 rpm for 5 minutes in order to pellet cell debris. The resulting supernatant was aliquoted and frozen at -80 C. Vero cells at 80% confluency were infected at MOI of 0.05 Ribavirin was added at the 2x concentrations stated in sample name in a volume of 750µl. Following incubation at 37°C for one hour, another 750µl MEM supplemented with 2% FBS was added to each well to reach the desired concentration. Virus containing culture media was harvested at 48 hours post-infection. RNA was extracted using the Qiagen QIAamp Viral RNA kit. cDNA libraries were prepared using random hexamer priming (TruSeq RNA Library Prep Kit)
Department of Pathology,  University of Texas Medical Branch
E-MTAB-8504:Asibi_0_0_um_p