GSM1847156: 9.8h_mO2_1; Pseudomonas aeruginosa PAO1; RNA-Seq
Cells were spun-down, then resuspended in 1 ml QIAzol lysis reagent (Qiagen) and disrupted by bead beating at maximum rpm for 3 - 1 min intervals with rests on ice with a Mini-Beadbeater-24 (BioSpec). RNA was then isolated using a miRNeasy Mini Kit (Qiagen), then DNA-digested with TURBO DNase (Life Technologies) followed by another purification step using a RNeasy MinElute Cleanup Kit (Qiagen). Ovation Complete Prokaryotic RNA-Seq DR Multiplex System (NuGEN)
GEO
GSM1847156