GSM4593851: abaI::Tn26 2; Acinetobacter baumannii; RNA-Seq
Total RNA was extracted using the RNAeasy Mini kit (QIAGEN) following the manufacturer instructions. RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Agilent  Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Sequencing libraries were generated using NEBNext® UltraTM Directional  RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's  recommendations and index codes were added to attribute sequences to each sample.  mRNA was purified from total RNA using poly-T oligo-attached magnetic beads.  Fragmentation was carried out using divalent cations under elevated temperature in  NEBNext First Strand Synthesis Reaction Buffer(5X). First strand cDNA was  synthesized using random hexamer primer and M-MuLV Reverse Transcriptase(RNase H). Second strand cDNA synthesis was subsequently performed using DNA Polymerase I  and RNase H.
GEO
GSM4593851