GSM4933464: KO, AORTA; Mus musculus; RNA-Seq
Aortas, including arch, thoracic, and abdominal portions and lung left lobes from EC-Oct4Δ/Δ (n = 3) and EC-Oct4+/+ (n = 2) female mice after 5 weeks of WD feeding were cleaned, cut, and digested with all buffers and solutions contained 1 µg/mL Actinomycin D (Gibco, 11805017) to prevent transcriptional changes induced by digestion. LoBind pipet tips (Xtip Biotix, R1000-9FC) were used to transfer samples. The cell suspension was transferred to 0.04% Ultra-Pure nonacetylated BSA (Thermo Fisher, AM2616) in PBS, filtered through 35 µm filters (Falcon, 352235) on ice, and sorted on a BD Influx sorter with the 100 µm nozzle into LoBind tubes (Eppendorf, 022431048) containing PBS with 0.04% Ultra-Pure nonacetylated BSA. Cdh5 negative littermate mice after 5 weeks of WD feeding were used as a control to set the YFP+ gate. The cells in each group were captured in Chromium 10X genomics libraries, which after barcoding, were pooled and sequenced on the Illumina MiSeq platform, 150 cycle high-output.
GEO
GSM4933464