Illumina MiSeq sequencing of SAMD00065478
The marine sediment PCR products were pooled after purification with AMPure XP Kit (Beckman Coulter) and the pooled library was subjected to an Illumina Miseq run with the 2 x 300 cycle sequencing kit (version 3). The remaining forward and reverse sequencing reads were then fused with each other by the program PEAR v0.9.6 (Zhang et al.,2014) with a stringent threshold for merging (p = 0.0001). Among the paired-end reads obtained, we discareded those less than 150 bp in length and those for which 10% or more of nucleotides had low (less than 30) quality values.
PHYTOROX
DRX069339