GSM5222764: normoxia_1hr_U2OS_biorep1_ChIP2; Homo sapiens; ChIP-Seq
Cell nuclei were extracted and sonicated. G4 containing DNA were respectively isolated with BG4 antibody. For G4 ChIP libraries, DNA was tagmented and the library was prepared using Nextera library preparation kit (20034197) by following the manufacturer instruction. The library was amplified using Nextera index primers (Illumina, FC-121-1030). For RNA Pol II ChIP-seq, library was prepared by using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (E7645). G4 ChIP-seq
GEO
GSM5222764