GSM3453210: ex vivoT naive donor 2; Homo sapiens; RNA-Seq
Total RNA was isolated from FACS sorted cells using the RNeasy Allprep DNA/RNA Micro Kit (Qiagen) cDNA was synthesized and amplified using the template switching technology of the SMART‑Seq v4 Ultra Low Input RNA Kit (Clontech Laboratories), followed by purification using the Agencourt AMPure XP Kit (Beckman Coulter). Library preparation was performed with Nextera XT DNA Library Prep Kit (Illumina).
GEO
GSM3453210