GSM3359493: Untreated_Control_1; Theileria annulata; Bos taurus; RNA-Seq
RNA isolation from the untreated and curcumin treated bovine leucocytes was performed using Trizol reagent as per standard protocol. The purity and concentration of total RNA extracted was checked using Nanodrop spectrophotometer by using 1 µl of total RNA against nuclease free water as blank to determine the absorbance ratio 260/280 nm. The quality check of the isolated RNA was performed in Agilent 2100 Bioanalyzer. RNA with RNA integrity number (RIN) greater than 7 was used for sequencing. Sequencing libraries were constructed following NEBNext® Ultra™ Directional RNA Library Prep Kit protocol and were then processed by Illumina HiSeq Paired- end sequencing.
GEO
GSM3359493