GSM1575694: Hi-C S2 MSL3 RNAi 1; Drosophila melanogaster; OTHER
Hi-C was carried out as described (Belton et al., 2012) with a few modifications to account for Drosophila cell and genome size: 50 million fixed insect cells per sample were used and batches of 20 million cells each were digested with 400U HindIII as Hi-C samples with a control 3C sample of 10 million cells. Cohesive ends in Hi-C samples were filled in with dATP, dGTP, dTTP and biotin-14-dCTP. Then DNA was ligated, purified and sonicated. Size selection for ~230bp fragments carried out and then enriched for biotinylated specimen with straptavidin beads. Illumina paired-end adapters and PfuUltra II Fusion DNA polymerase were used for library generation. For all libraries 10 cycles of PCR amplification were used.
GEO
GSM1575694