GSM2101279: shISL1 RNA-seq; Mus musculus; RNA-Seq
RNA was harvested using Trizol reagent.1 ug of total RNA was used for the construction of sequencing libraries. Libraries were prepared according to manufacturer’s instructions (Illumina). Briefly, polyA+ RNA was isolated using Dynabeads Oligo(dT)25 (Invitrogen) and constructed into strand-specific libraries using the dUTP method. Once dUTP-marked doublestranded cDNA was obtained, the remaining library construction steps followed the same protocol as described above for ChIP-seq libraries.
GEO
GSM2101279