GSM2802545: WT2_TOTL; Mus musculus; RNA-Seq
Livers were collected from 12 week-old mice. Mice were sacrificed at 9 PM and livers were directly lysed in the polysome buffer and kept on ice for 10 min. Samples were centrifuged at 3000 rpm during 3 min and the supernatants were used for library preparation. Similar material extracted from Drosophila S2 cells was used as a spiking control and added to the mouse material at a ratio of 1/15. Thirty OD of the spiked material was used for library preparation. Libraries of Ribo-seq and total RNA-seq were prepared according to the Illumina TruSeq Ribo Profile Mammalian protocol. The Truseq Ribo profile Nuclease was replaced by the RNase I from Ambion (Cat N. AM2295) to generate the ribosome footprinting.
GEO
GSM2802545