RNAseq of Mycamoeba gemmipara
About 50 M. gemmipara cells were scraped off of an agar plate using a 30-gauge platinum wire placed directly into a 200 L thin-walled PCR tube. The cells were subjected to a  modified version of Smart-Seq2 that includes an additional freeze thaw step for cell lysis described in Onsbring et al. 2019 for mRNA extraction and cDNA library preparation. The resulting cDNA libraries were prepared for sequencing on the Illumina platform using a Nextera XT DNA Library Prep Kit (Illumina, CA) following the manufacturer's protocol with dual index primers. The M. gemmipara library was pooled with libraries for an unrelated study and sequenced using an Illumina HiSeq 4000 at Genome Quebec.
SUB9530496
Mycagemm