GSM5077387: N3752_WT_BSseq; Neurospora crassa; Bisulfite-Seq
Genomic DNA was isolated using a protocol modified from (Oakley et al, 1987). Genomic DNA (50ng) was treated with bisulfite using the EZ DNA Methylation LightningTM kit (ZYMO Research) per the manufacturer's protocol. Kit (Epicentre cat #EGMK91324) and EpiGnomeTM Index PCR Primers (Epicentre cat# EGIDX81312) per the manufacturer's protocol, purified with Agencourt AMPure XP beads (Beckman Coulter) per the manufacturer's protocol, Qubit HS Assay quantified (Life Technologies) per the manufacturer's protocol, visualized on the Fragment AnalyzerTM (Advanced Analytical) per the manufacturer's protocol, and sequenced on a Illumina HiSeq 2000
GEO
GSM5077387