GSM2199343: RNA-seq_singleSort_nrlKOPhotoreceptor_1; Mus musculus; RNA-Seq
Retinas were harvested from eight-week-old mice, dissociated with trypsin, washed with calcium- and magnesium-free HBSS, and resuspended in FACS buffer (1% FBS, 0.1mM EDTA in calcium- and magnesium-free HBSS). GFP-positive cells were isolated via FACS and collected in Buffer RLT (Qiagen). cDNA was prepared using the SMARTer Ultra Low RNA Kit for Illumina Sequencing - HV (Clontech) per manufacturer's instructions. Subsequent preparation of RNA-seq libraries was performed using standard Illumina protocols.
GEO
GSM2199343