RNA-Seq of Boechera divaricarpa: ES517, nucelli and apomictic initial cells
Laser-assisted microdissection was used to isolate nucelli from developing ovules harbouring the megaspore mother cell or apomictic initial cell. Nucellus tissues were precisely dissected from dry sections of young floral buds. RNA was extracted, amplified, and subjected to library preparation using PicoPureTM RNA isolation kit (Thermo Fisher Scientific), SMARTseq v4 Ultra Low Input RNA Kit for Sequencing (Takara Bio USA, Mountain View, USA), and Nextera XT DNA Library Prep Kit (Illumina, San Diego, USA), respectively. RNA-Seq was performed using the single-end 75 bp protocol with 19 additional cycles on one lane of the Illumina NextSeq 500 platform.
SUB4469559
ES517_2