16S rRNA gene Illumina sequencing of healthy and bleached Delisea pulchra algae collected at Bare island, Sydney, Australia
Five healthy and three bleached Delisea pulchra individuals were collected in a single day during the 2008 austral summer at Bare Island, Sydney, Australia. Each algal individual was washed with filtered seawater and DNA was extracted from freeze-dried algal samples using a ZR Soil Microbe DNA extraction kit (Zymo). DNA samples were processed by the Earth Microbiome Project to generate amplicons for the variable region 4 (V4) of the 16S rRNA gene using the universal bacterial/archaeal primers 515F/806R. The amplified fragments were sequenced on a HiSeq Illumina 2000 platform with 100 bps from the 515F primer.
Centre for Marine Bio-innovation, University of Ne
16S_Dpulchra_2008_BI