Liver tissue of low-dose DEN rat treated with AM095, group 1 (lowdoseDEN_AM095_1)
Male Wistar rats (Charles River) received weekly i.p. injections of diethylnitrosamine (DEN) at 50 mg/kg for 18 weeks. AM063 (5mg/kg), AM095 (30mg/kg), or vehicle was given via gavage 5 days/week started at the first sign of fibrosis (beginning of week 9) and continued for a total of 10 weeks (n=9 for each group). Rats were sacrificed at the end of week 19 after a one-week washout period to eliminate acute effects of DEN. Two animals were randomly chosen for each group and pooled for RNA-Seq (i.e., each bar code corresponds a pool of two animals in an experimental group except lowdoseDEN_AM095_2).
Icahn School of Medicine at Mount Sinai
AM95_3_4__DEN283_275_index2