GSM4257303: cut14+ biorep 1; Schizosaccharomyces pombe; Hi-C
Crosslinked chromatin was digested by DpnII. The sticky ends were filled in by Biotin-14-dATP and the other dNTPs. Proximal ligation was performed, followed by DNA recovery by Biotin-Streptavidin interaction. NEBNext Ultra DNA Library Prep kit for Illumina and NEBNext Multiplex Oligos for Illumina (Index Primers Set 1) were used for adapter ligation and indexing. Indexed Hi-C library was purified by double selection of SPRI selection beads.
GEO
GSM4257303