GSM5170187: mRNA_549_Veh_rep_1; Homo sapiens; RNA-Seq
For mRNA-seq samples, RNA was extracted using RNease Mini Kit (Qiagen) according to the manufacturer protocols. For CRISPR screen samples, genomic DNA was extracted using Quick-DNA Midiprep Plus kit (Zymo Research). mRNA-seq libraries were prepared using the TruSeq RNA Library Preparation Kit (Illumina) according to the manufacturer's instructions. CRISPR screen libraries were prepared uing PCR primers with barcode which can amplify sgRNA sequences.
GEO
GSM5170187