GSM3455507: Microglia_shScrmbl_1; Mus musculus; RNA-Seq
Each CNS was isolated per mouse. A cell suspension was generated by trypsinization, neutralization, and 70um filtration of cell extract. Cells were centrifuged, resuspended in 30% Percoll, centrifuged, washed in PBS, and stained with primary antibodies for flow cytometry. Once cells were sorted, cells were pelleted, lysed, and RNA was harvested according to the Qiagen RNeasy Mini Kit protocol. Libraries were processed by the Broad Institute's Broad Technology Labs and the Broad Genomics Platform. Libraries were prepared according to the 3' Digital Gene Expression (3' DGE) protocol (Soumillon et al., 2014)
GEO
GSM3455507