GSM1215106: cumulus cell (CC) M2 rep 3; Homo sapiens; RNA-Seq
Total RNA was extracted from CCs by the Mini RNA Isolation I Kit (Zymo Research Corp., CA), according to manufacturer’s instructions. Following isolation total RNA was tested with a Bioanalyzer (Agilent Technologies 2100). mRNA was purified from Total RNA using pre-prepared Sera-mag Magnetic Oligo(dT) Beads (Thermo Fisher Scientific, MA). RNA prepared for sequencing according to the standard Illumina protocols (Illumina inc.San Diego, CA) for sequencing of cDNA samples.
GEO
GSM1215106