GSM2803427: SF4_mouse_atacseq; Mus musculus; ATAC-seq
Cells were lysed to release nuclei, then resuspended in a transposition buffer containing Tn5 Transposase for 30 minutes at 37 ºC prior to eluting DNA fragments using the Qiagen™ MinElute Kit. The Nextera library was generated using an Illumina Nextera DNA Library Prep Kit (San Diego) after 9 PCR cycles for library amplification and indexing. Following amplification, samples were sequenced using an Illumina HiSeq platform to generate ~75 M pair-end 2x100 bp reads.
GEO
GSM2803427