GSM1213858: Early maturation-stage seed coat [small-RNA]; Glycine max; RNA-Seq
Total RNA was isolated from three seed parts (axis, cotyledon, and seed coat) using the Concert Plant RNA Reagent (Invitrogen, Carlsbad, CA) according to manufacturer’s instructions. The TruSeq Small RNA Sample Preparation kit was used to generate libraries with modifications (Illumina, San Diego, CA).  Specifically, 250 ng total RNA was used as input and 15 PCR cycles was used for the final PCR enrichment step. Phi-X174 DNA was spiked in to the library by the sequencing facility before cluster formation and sequencing.
GEO
GSM1213858