lncRNA-seq of humanHCC cell lines
First strand cDNA and double strand DNA (dsDNA) were subsequently synthetized using reverse transcriptase (SuperScript III, Invitrogen), random primers, and dNTPs. Thereafter, cDNA samples were purified and ligated to adenylate 3-ends and sequencing adaptors, followed by purification (XP beads, Beckman Coulter, Brea, CA, USA). Next, dsDNA containing uracil deoxyribonucleotides (U base) was degraded. Finally, PCR enrichment followed by purification was performed to obtain sequencing libraries.
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