GSM5169194: LDLR KO_1_LPS 4 hours; Mus musculus; RNA-Seq
After the treatment thioglycollate-elicited peritoneal macrophages were harvested and RNA was isolated Total RNA from liver samples treated with ASOs as described above was extracted and purified using a RNA isolation Kit (Qiagen) followed by DNAse treatment to remove genomic contamination using RNA MinElute Cleanup (Qiagen). rRNA was depleted from RNA samples using Ribo-Zero rRNA Removal Kit (Illumina). RNA libraries were performed using TrueSeq Small RNA Library preparation (Illumina). Libraries were sequenced for 45 cycles on Illumina HiSeq 2000 platform (1 x 100bp read length).
GEO
GSM5169194