RRBS analysis of Eset floxed ES cells
DNA extracted from Eset floxed ES cells were first digested with MspI restriction enzyme, followed by the ligation with methylated adaptors at the both end of DNA fragments. DNA fragments of 150-250bp in length were then subjected to bisulfite conversion and amplified by PCR. Sequence was performed at 36bp single end protocol under illumina platform.
HIROSHIMA
DRX016198