total RNA: EBOV-Minigenome with NheI-Mutation transfected HEK293 cells
Poly(A)+ RNA was isolated from total RNA samples and fragmented with ultrasound (2 pulses of 30 sec at 4C). First-strand cDNA synthesis was primed with a N6 randomized primer. Then, the Illumina TruSeq sequencing adapters were ligated to the 5' and 3' ends of the cDNA. The cDNA was finally amplified with PCR using a proof reading enzyme. For Illumina sequencing, the cDNAs in the size range of 400 - 600 bp were eluted from preparative agarose gels.
SUB9183141
NheI_plusVP30_total_T1484_S3