16S Rdna of environmental sample
Biofilm samples in the bioreactor were collected and their total genomic DNA was extracted using the FastDNA Spin kit (MP Biomedicals USA) according to the manufacturer's guidelines. The extracted DNA was then amplified with PCR primers 338F (50-ACTCCTACGGGAGCAG-30) and 806R (50-GGACTACHVGGGTWTCTAAT-30). The amplified sample mixture was used for high-throughput 16S rDNA gene analysis by MiSeq (Illumina, USA), conducted by Shanghai MajorBio Technology (Shanghai, China).
SUB10019542
environmental sample