Global expression profiling of goat mammary glands induced by xanthosine administration.
Illumina HiSeq 2500, Paired end run; 2 X 100 bp. ~5-10 ug of total RNA was used to prepare the RNA seq library using the TruSeq RNA Sample Prep Kits (Illumina). The libraries were prepared as per the kit protocol. Following purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperature. The cleaved mRNA fragments was used to synthesize first strand cDNA synthesis using DNA polymerase I and RNaseH. These cDNA then proceed through an end repair process, the addition of a single 'A' base and ligation of adapters. The prodcuts were purified and enriched with PCR to create the final cDNA library.
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