GSM3666334: 03_037F_00EEGothen_mTC31_BACH1_mm_i92; Mus musculus; ChIP-Seq
Lysates were sonicated and the DNA sheared to an average length of 300–500 bp. Genomic DNA (input) was prepared by incubating chromatin aliquots with RNase, proteinase K and heat for de-crosslinking, followed by ethanol precipitation. Pellets were resuspended and the resulting DNA was quantified on a NanoDrop spectrophotometer. Extrapolation to the original chromatin volume allowed quantitation of the total chromatin yield. An aliquot of chromatin (30 mg) was pre-cleared with protein G agarose beads (Life technologies). Genomic DNA regions of interest were isolated using 4 mg antibody recognizing BACH1 (AF577, R&D Illumina sequencing libraries were prepared from the ChIP and input DNAs by the standard consecutive enzymatic steps of end-polishing, dA-addition, and adaptor ligation. After a final PCR amplification step, the resulting DNA libraries were quantified and sequenced on Illumina's NextSeq 500 (75-nt reads, single end).
GEO
GSM3666334