GSM3291226: 7dSS_12-2; Homo sapiens; RNA-Seq
Proliferating, 7dCI, and 7dSS fibroblasts were lysed using 1 mL of Trizol reagent (Life Technologies, Carlsbad, CA) per 10 cm plate for 5 minutes.  RNA was isolated from Trizol lysates following manufacturer instructions. RNA concentrations were determined using a Nanodrop Spectrophotometer (Thermo Fisher Scientific Inc., Waltham, MA). RNA quality was verified on a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA) using reagents from the RNA Nano 6000 kit (Agilent Technologies). cDNA libraries containing fragments enriched for 3´ ends of transcripts were created with the Gnomegen RNA-Seq Library Preparation Kit for RNA profiling (Gnomegen, San Diego, CA). Libraries with a uniform size distribution between 200 and 1000 bp were subjected to gel size selection to enrich for 200 – 300 bp sized fragments. Single-end 140 bp reads were generated on an Illumina HiSeq 2000 Instrument. The sequencing reaction was run for 147 cycles.
GEO
GSM3291226