Hybridization-based viral enrichment sequencing of bat guano spiked with 1250 genome equivalents of Influenza virus
TruSeq RNA Access libraries were created as per manufacturers protocol, with the following two modifications: i) rather than the standard CEX oligonucleotides that are designed for enrichment of human genes, a custom pool of oligonucleotides was used that includes probes along the entire genome length of 83 viruses as well as probes specific for several human house-keeping genes, and ii) in the second PCR amplification, 17 cycles were used rather than ten.
SUB4590619
1250GE_probed