RNA-Seq of ticks from China
Each three groups of ticks from the same location and same year were mixed as a pool for subsequent metagenomic RNA-seq. Firstly ticks were homogenized at 4000 cycles/sec by disruptor machine with 1ml PBS, and then centrifuged at 5000g for 10 min at 4 to remove the cellular debris. The 300 ul supernatant was transferred to a RNase-free tube then subject to RNA extraction using TRIzol LS reagent (Invitrogen, Calrsbad, CA). Finally, RNA was used for library construction according to protocol, including fragmentation, reverse-transcribed, ends repairation, adaptor ligation, purification.
SUB4424269
2015-9YNT