Carex microsatellites
This study reports 109 microsatellite loci obtained by 454-sequencing. We construct a shotgun genomic library that was sequenced on 1/4th of a plate using 454 GS FLX Titanium chemistry at the University of Arizona Genetic Core (Tucson, Arizona, USA). We searched for all possible microsatellite loci containing at least six perfect repeats for hexa-, penta-, tetra-, and trinucleotides or 12 perfect repeats for dinucleotides and designed primers using the software QDD version 3 ( Meglécz et al., 2014 ).
Estación Biológica de Doñana
Carex microsatellites