RNA-Seq of A549 cell line
RNA-seq was performed with BCAT1overexpression and control A549 cells (each with three replicates) using BGISEQ-500 sequencing system. In brief, RNA sample was extracted and the quality was assessed, followed by generation of cDNA libraries. Gene expression levels for each sample were quantified using the FPKM method (fragments per kilobase of transcript per million mapped reads) and processed for analysis. DEGs were screened by fold change  2 and the adjusted p value  0.01. Gene Ontology (GO) annotation proteome was performed from the UniProt-GOA database (http://www.ebi.ac.uk/GOA). Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was based on database (http://www.genome.jp/kegg/).
SUB9356103
A549 control3