NextSeq 500 sequencing
RNA­seq of Bacillus cereus RSVF1 infected with phage Wbeta, 20 minutes post infection, with addition of the arbitrium peptide to the growth medium
Ribosomal RNA was depleted by using the Ribo­Zero rRNA Removal Kit (epicentre, MRZB12424). Strand­specific RNA­seq was performed using the NEBNext Ultra Directional RNA Library Prep Kit (NEB, E7420) with the following adjustments: all cleanup stages were performed using ×1.8 SPRI beads, and only one cleanup step was performed after the end repair step.
WEIZMANN INSTITUE OF SCIENCE
ena-EXPERIMENT-WEIZMANN INSTITUE OF SCIENCE-11-03-2019-13:10:39:972-3