GSM5032711: WT BL6 [WGS]; Mus musculus; OTHER
DNA was extracted from cells using the Qiagen DNeasy Blood and Tissue kit  Next Generation sequencing libraries were prepared using Illumina SeqLab specific TruSeq PCR-Free High Throughput library preparation kits in conjunction with the Hamilton MicroLab STAR and Clarity LIMS X Edition, following standarad protocols. The gDNA samples are normalised to the concentration and volume required for the Illumina TruSeq PCR-Free library preparation kits then sheared to a 450bp mean insert size using a Covaris LE220 focused-ultrasonicator. The inserts are blunt ended, A-tailed, size selected and the TruSeq adapters are ligated onto the ends.
GEO
GSM5032711