seawater wastewater microcosm_3 at day 16
PCR amplification were conducted in triplicate reactions using the 515f/806r primer to amplify the V4-V5 region of the 16S rRNA gene. Amplicons of the triplicate PCR reactions were pooled and then sequenced using an Illumina HiSeq 2000 instrument.
University of Hawaii at Manoa
SWWW16_3