GSM3415455: HS16; Homo sapiens; RNA-Seq
Total RNA from whole human skin, and HaCaT keratinocyte cell cultures were isolated with RiboPure kit (Ambion, Life Technologies, Grand Island, NY, USA). The RNA samples were treated with TURBOTM DNase (Ambion), checked for quality and integrity with the Agilent 2100 bioanalyzer and used for RNASeq. Due to the conical shape of punch skin biopsies, the RNA was mostly extracted from keratinocytes with minimal contribution of dermal cells RNA libraries were prepared for sequencing using standard Illumina protocols
GEO
GSM3415455