GSM2803881: VDA_24h_n=2; Verticillium dahliae; RNA-Seq
Both, RNA of plant and fungus, was isolated from 100 mg root material (≈ 120 seedlings per replicate). The frozen roots were finely ground with mortar and pistil and weighted. The RNA from the samples was extracted with peqGOLD TriFast™ FL (VWR, Darmstadt, Germany) according to the manufacturer’s protocol.  The RNA was further processed by use of the PureLink™ RNA Mini Kit (Thermo Fisher Scientific, Dreieich, Germany) with on-column DNase treatment. RNA was dissolved in water and checked for quality. The isolated RNA was shipped to GeneCore (Heidelberg, Germany) where library construction was performed using the mRNA sequencing Sample Preparation Guide (Illumina, Cat#RS-930-1001), followed by a validation of the library and RNA sequencing (Hi Seq 2000, single-end 75 bp, ~50 Mill. reads/sample).
GEO
GSM2803881