GSM1574080: prdm12_engR_RNAseq_rep_2; Xenopus laevis; RNA-Seq
mRNAs were isolated with an RNAspin Mini RNA isolation kit. ChIPseq embryos were first fixed in 1% formaldehyde for 30 minutes and lysed in RIPA buffer prior to sonication. See PMID: 24934224 for a more detailed protocol. RNAseq libraries were constructed with Illumina Truseq v2 non-stranded mRNA kits. Following IP, ChIPseq libraries were constructed with end repair mix (NEB), a-tailed (NEB), Truseq v2 adapters ligated (Illumina, NEB) and amplified with Phusion polymerase (NEB).
GEO
GSM1574080