GSM4989305: S2KO_Rep1; Homo sapiens; RNA-Seq
total RNA mRNA was purified from total RNA using poly-T oligo attached magnetic beads and 0.8ug RNA was reverse transcribed using random hexamer primers to generate first strand cDNA. Second second cDNA was generated, purified and overhangs were converted into blunt ends. Ends were then adenylated and NEBNext adaptor was ligated for hybridization and PCR amplified for 12 cycles. Sequencing was performed using an Illumina Hiseq 4000 Platform with 2x150 paired-end (PE150) sequence reads.
GEO
GSM4989305