RNA-Seq of Chorthippus rubratibialis: adult male
Tissue samples were preserved in RNAlater (Qiagen). To sample the largest variety of transcripts possible, we extracted total RNA from whole body, after removing the head and digestive tract of each individual to avoid over-representation of eye pigments and gut contaminants. We homogenized the samples using ceramic beads 1.4/2.8mm (Precellys and the standard Tri-Reagent protocol (Sigma). Resuspended RNA pellets were purified with RNAeasy Mini columns (Qiagen), and final sample integrity and quantity was assessed with an Agilent 2100 BioAnalyzer. Enrichment for mRNA, library construction, and paired end Illumina HiSeq2500 sequencing was performed by the BGI.
SUB8135380
Crub_Im2