GSM3455443: Control-B1; Homo sapiens; ncRNA-Seq
Total RNA was isolated from vascular tissue with Trizol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's protocol. Small RNA (135-170bp) were obtained by standard denaturing by 1% agarose gel electrophoresis. Purified small RNA was then sent to Yingbio (Shanghai, China) for construction of small RNA libraries using NEBNext® Multiplex Small RNA Library Prep Kit (NEB #E7560S) and for small RNA sequencing analysis The total RNA was removed from rRNA. Thereafter, cDNA constructs were created using RT-PCR. Next, the dA-tailing reagent was added and 3'- and 5'- adapters were purified. Finally, RNA libraries were sequenced on an Illumina NextSeq 500.
GEO
GSM3455443