GSM5031710: Balbc Adult mouse mammary gland; Mus musculus; RNA-Seq
The Balbc mammary glands were placed in 10 ml of a digestion medium con-taining EpiCultTM-B Mouse Medium Kit (#05610, StemCell Technologies), Col-lagenase/Hyaluronidase (#07912, StemCell Technologies) and 1% penicillin streptomycin (Gibco). The mammary gland was digested overnight in a thermocycler maintained at 37 C with continuous rotation. RNA was isolated from the cell-lines and the mammary glands using the RNeasy Mini Kit (#74104, Qiagen) according to manufacturer protocol. scRNA-Seq libraries were prepared following the Single Cell 30 Reagent Kits v3 User Guide (Manual Part # CG000183 Rev C) using the following Single Cell 30 Reagent Kits v3: ChromiumTMSingle Cell 3' Library & Gel Bead Kit v3, PN-120237, Chromium Single Cell B Chip Kit, 16 rxns PN-1000154 (America and Asia Pacific), and i7 Multiplex Kit PN-120262 (10X Genomics). scRNA-Seq Libraries were run on an Illumina HiSeq 4000 as 2 150 paired-end reads.
GEO
GSM5031710