GSM2102413: P20_Liv_5_LSBS; Mus musculus; OTHER
DNA was extracted using a Qiagen all-prep DNA/RNA kit RRBS libraries were constructed using the Truseq DNA LT kit, with a bisulfite treatment after adapter ligation. For locus specific bisulfite sequencing, we bisulfite treated DNA and PCR amplified regions of interest using primers with a 5' tail. A second PCR recognizing the 5' tail added barcode and adapter sequences.
GEO
GSM2102413