GSM5344476: TM016; Homo sapiens; RNA-Seq
Total RNA from around 0.5-1x10^6 cells was isolated with Trizol (Sigma), digested with TurboDNase (Ambion), washed and resuspended in H2O. The RNA integrity was confirmed with an RNA Bioanalyzer (Agilent). Comparable amounts (250ng – 1ug) of total RNA was converted into non-poly(A) selected, ribosomal RNA depleted (TruSeq RNA Library Prep Kit v2) cDNA libraries following Illumina protocols. RNA libraries were prepared for sequencing using standard Illumina protocols.
GEO
GSM5344476