ATAC-seq of Arabidopsis thaliana : 8 day old whole seedling
Sorted nuclei were pelleted by centrifugation at 1000 g at 4C for 10 min and washed by Tris-Mg buffer (10 mM Tris-HCl pH 8.0, 5 mM MgCl2) twice. The pellet was resuspended with 1.5 l Tn5 transposomes, 15 ul tagment buffer and 13.5 ul water, and incubate at 37C for 30min. Tn5 transposomes and tagment buffer were from Nextera DNA Library Prep kit (Illumina). After integration, the products were purified with Minielute kit (QIAGEN) and then amplified using NEBNext High-Fidelity PCR Master Mix (NEB) for 12 cycles. The number of PCR cycle was determined. Sequencing was done on HiSeq3000 platform using 2  101 cycle multiplex paired-end reads.
SUB8624355
ATACseq_Cold_22C_Rep2