GSM5342497: WGBS_Sscr_fibro; Sus scrofa; Bisulfite-Seq
Genomic DNA was extracted using the AllPrep DNA/RNA Mini Kit (Qiagen). 100 ng of genomic DNA were fragmented to 350 bp using a Covaris E220 sonicator. Bisulfite conversion was performed with the EZ DNA Methylation-Gold kit (Zymo), and WGBS libraries were prepared using the Accel-NGS Methyl-Seq DNA library Kit (Swift Biosciences) following the manufacturer's instructions. 4 to 6 cycles were used for the final PCR amplification of the WGBS libraries. The libraries were purified using Agencourt AmpureXP beads (Beckman-Coulter).
GEO
GSM5342497