GSM1853584: siDIS3_rep3; Homo sapiens; RNA-Seq
For RNAseq experiments, individual replicates were collected and extracted using the RNAqueous kit (Life Technologies #AM1912). DNase digestion was performed twice, once in batch and once on the column, using the RNAeasy kit (Qiagen #74104). RNA libraries were prepared for sequencing using standard ribo-depleted Illumina protocols.  We sequenced using the Illumina HiSeq, outputting single end, 50 bp, stranded RNAseq reads
GEO
GSM1853584