RNA-Seq of Ocimum basilicum Genovese: mature leaf CO2 + LT Rep 2
Total RNA was extracted from 100 mg of leaf tissues from sweet basil (Ocimum basilicum) using the modified hot borate protocol (Wan and Wilkins, 1994). A total of 30 RNA samples were extracted, including 3 biological replicates from each treatment and total of 10 treatment groups. The contaminated genomic DNA present in each RNA sample was treated with DNase I (Promega, Madison, WI, USA) using in-solution DNase I digestion procedure, followed by the cleanup procedure using Qiagen RNeasy Mini kit (Qiagen, Valencia, CA, USA). The quantity of RNA was determined by the Nanodrop One Spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA), and the quality of RNA was confirmed by agarose gel electrophoresis before sending to company (Novogene, Sacramento, CA, USA) for the directional RNA-Seq library preparation and Illumina Pair-end 150-bp (2x150) HiSeq sequencing.
SUB8249861
SBL_10_2