GSM5032462: KO_D0_iPS_Rep1; Homo sapiens; Hi-C
cHi-C libraries were prepared from wild type or homozygous SOX17∆5'CTCF iPSC or dEN cells. Undifferentiated or day 5 differentiated ZIP13K224 cells were grown to a final count of 4-5 million, treated with Accutase (Sigma-Aldrich, A6964), resuspended and washed in DPBS. Cell lysis, NlaIII (NEB R0125) digestion, ligation and de-crosslinking was performed according to the Franke et al. protocol25. Adaptors were added to DNA and amplified according to Agilent instructions for Illumina sequencing. The library was hybridized to the custom-designed sure-select beads and indexed for sequencing of 200x106 fragments per sample (100 bp paired-end) following the Agilent instructions. Capture Hi-C experiments were performed as biological duplicates.
GEO
GSM5032462