18S rRNA amplicon sequencing of human stool sample from caesarean section born neonate around day 3 after birth
The V4 region of the 18S rRNA gene was amplified by using primers 574*F and 1132R targeting eukaryotes. 25 amplification cycles were employed. Sequencing with 2 x 300 nt was performed using the V3 MiSeq kit on a MiSeq platform (Illumina). Amplification and sequencing was performed by the Groupe Interdisciplinaire de Génoprotéomique Appliquée (GIGA; Liège, Belgium).
SUB2679173
V2_C118_18S