GSM5176627: CTCF_NICD_TCF_rep1; Mus musculus; ChIP-Seq
Chromatin immunoprecipitation followed by sequencing (ChIP-seq) was performed on chromatin from 20,000 sortedLSK cells. Sorted cells were cross-linked with 1% methanol-free formaldehyde (Pierce Life Technologies Cat# 28906), quenched with 0.125 M glycine and frozen at -80 °C and stored until further processing. ChIP reaction was performed with Diagenode True MicroChIP Kit (Diagenode Cat# C01010130) with modifications of the manual detailed below. Lysed samples were sonicated using Diagenode Bioruptor Pico (Diagenode Cat# B01060010). ChIP and input libraries for sequencing were prepared with MicroPlex Library Preparation Kit (Diagenode Cat# C0101134). Size selection steps were performed with Agencourt AMPure XP magnetic beads (Beckman Coulter Cat# A63880). sequencing protocol: The libraries were sequenced by Gene Expression Core Facility of EPFL using the Illumina NextSeq 500 platform and the 75-bp paired-end configuration to obtain at least 30 million reads per sample.
GEO
GSM5176627