RNA-Seq of Triturus karelinii: adult male dorsal gland (cloaca)
One captive-bred male Triturus karelinii was euthanised by spraying 0.2-0.5 ml of a 10% lidocaine solution on the head followed by decapitation and pithing of the brain. Pheromone-producing glands were excised from the body cavity and stored in RNALater solution (Sigma-Aldrich). Total RNA was extracted from the pheromone-producing glands using the RNeasy Plus Universal Mini Kit (Qiagen). Whole transcriptome shotgun sequencing (RNA-Sequencing, paired-end, 159 Mio 100 bp read pairs) was performed at DNAVision (Gosselies, Belgium), using an Illumina TruSeq RNA sample preparation kit for sequencing on the Illumina NextSeq 500 platform (Illumina, San Diego, California). The raw data from Illumina NextSeq were deposited in the NCBI Short Read Archive (SRA) database.
Vrije Universiteit Brussel (VUB)
TriKar_AEL043