GSM4403038: H3K27me3_ChIPseq_WT_2; Mus musculus; ChIP-Seq
For ChIPseq, cells were fixed with 1% formaldehyde and insoluble chromatin fraction were isolated. Chromatin were sonicated to 200 to 500 base pair and protein-DNA complexes were pulled down by antibodies. For ChIPseq samples, libraries were prepared using ThruPLEX DNA-seq kit (R400428) according to manufacturer's instruction. Constructed libraries were PAGE purified and only libraries with 250 to 500bp were used for sequencing.
GEO
GSM4403038