GSM5224019: aramisRNAi_input_1; Drosophila melanogaster; OTHER
RNA was isolated using TRIzol for input samples (Invitrogen, 15596026) or acid phenol-chloroform treatment for polysome samples, treated with DNase (TURBO DNA-free Kit, Life Technologies, AM1907).  mRNA-enriched libraries were obtained by treating total RNA with poly(A)tail selection beads following the manufacturer's instructions of the NEXTflex Rapid Directional RNAseq Kit (Bioo Scientific Corp, NOVA-5138-08) Following the manufacturer's instructions of the NEXTflex Rapid Directional RNAseq Kit (Bioo Scientific Corp, NOVA-5138-08), except RNA was fragmented for 13 minutes
GEO
GSM5224019