Llamara evaporites upper layers1 - Winter - 16S rDNA V4 region 454 sequences
The V4 hyper variable region of the 16s rRNA gene was amplified using primers containing a TAG sequence (19 nt, different sequences for the forward and reverse primer) and the universal primers obtained from RDP’s Pyrosequencing Pipeline (http://pyro.cme.msu.edu/pyro/help.jsp): (5’-CACGACGTTGTAAAACGACAYTGGGYDTAAAGNG-3’, 5’-CAGGAAACAGCTATGACCTACNVGGGTATCTAATCC-3’, 5’-CAGGAAACAGCTATGACCTACCRGGGTHTCTAATCC-3’, 5’-CAGGAAACAGCTATGACCTACCAGAGTATCTAATTC-3’, 5’-CAGGAAACAGCTATGACCCTACDSRGGTMTCTAATC-3’). A second PCR reaction was performed to add MIDs and Roche adaptors A and B. The forward primer contained the 454 adaptor A, a unique MID (5’-ACGAGTGCGT-3’), and the TAG Fw sequence. The reverse primer contained the 454 adaptor B and the TAG Rv sequence.
PROIMI
Llamara_1_winter_Upper_layer_1