GSM2974995: Developing seeds_input rep2; Arabidopsis thaliana; ChIP-Seq
Lysates were clarified from sonicated nuclei and DNA-protein complexes were isolated with antibody. Libraries were prepared according to the DNA Sample Kit . After adenylation of 3' ends of DNA fragments, NEBNext Adaptor with hairpin loop structure were ligated to prepare for hybridization. In order to select DNA fragments of preferentially 150~200 bp in length, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then 3 µl USER Enzyme (NEB, USA) was used with size-selected, adaptor-ligated DNA at 37°C for 15 min followed by 5 min at 95 °C before PCR. Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. At last,  PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system.Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols.
GEO
GSM2974995