GSM5179457: WT 1; Mus musculus; miRNA-Seq
We isolated the exosomes using the Umibio® exosome isolation kit (UR52121, Umibio, China). MiRNA was harvested using Trizol reagent for library construction. Total RNA of each sample was used to prepare the miRNA sequencing library, which included the following steps: 1) 3'-adaptor ligation; 2) 5'-adaptor ligation; 3) cDNA synthesis; 4) PCR amplification; 5) Size selection of ~150 bp PCR amplicons (corresponding to ~22nt miRNAs)
GEO
GSM5179457