GSM4593917: Ck_2; Brassica rapa subsp. pekinensis; RNA-Seq
Total RNA was extracted from the TuMV-infected and control leaves using TRIzol reagent (Takara Bio Inc., Otsu, Japan) following the manufacturer's protocol. RNA quantity and quality assessment were performed using the Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA) and gel electrophoresis. After treatment with DNase I and enrichment using the oligo (dT) magnetic beads (for eukaryotes), the mRNA was fragmented into short fragments (about 200 bp). Subsequently, double-stranded cDNA was synthesized, and the sequence adaptors were ligated to the fragments.
GEO
GSM4593917