GSM4815717: M5; Oreochromis niloticus; RNA-Seq
Fast muscle was stored in -80 C. RNA was extracted after homogenizing the tissue within the DNA/RNA Shield using the Quick-RNA miniprep plus kit (Zymo Research) according to manufacturer's protocol and recommendations. After extracting the total RNA as described above, rRNA depletion was performed using the Ribo-Zero Gold rRNA removal kit (Illumina) according to manufacturer's protocol RNA libraries were prepared using the NEBNext Ultra II directional RNA library prep kit for Illumina a ccording to manufacturer's protocol
GEO
GSM4815717