GSM5170573: Dnmt3a-KO with IFNg exposure; Mus musculus; Bisulfite-Seq
gDNA was extracted from 3x10^5 donor derived bone marrow cells at the conclusion of the primary transplant using PureLink Genomic DNA mini Kit (Invitrogen) 250ng of gDNA of each sample, spiked in with lambda control was fragmented using Covaris. The fragmented DNA was then subjected to an overnight cytosine-to-thymine conversion using EZ DNA Methylation-Gold Kit. The CT converted samples were used for library construction with Accel-NGS Methyl-Seq DNA Library Prep Kit, indexed with Methyl-Seq Dual Indexing kit. The sequencing was performed on NovaSeqS4 300XP.
GEO
GSM5170573