GSM1849097: Co_P1_FA_GFP_1; Mus musculus; RNA-Seq
Following dissociation of tissue into a single-cell suspension, target cells were enriched by purification as described, cells were lysed, polyA RNA reverse-transcribed, and cDNA amplified using SMARTer ultra-low input cDNA synthesis (Clontech) on the C1 microfluidics platform (Fluidigm). cDNA libraries were prepared for sequencing using Nextera XT tagmentation and 96-barcode indexing (Illumina). Barcoded samples were pooled, cleaned with Agencourt AMPure XP magnetic bead isolation (Beckman Coulter).
GEO
GSM1849097