GSM4989572: CLL_312_ATACseq; Homo sapiens; ATAC-seq
Briefly 50,000 cells were lysed for nuclei isolation using ATAC-Resuspention Buffer containing 0.1% NP40, 0.1% Tween-20, and 0.01% Digitonin. Transposition reaction was performed for 30 min at 37C using Nextera DNA library preparation kit (NEB). Transposed fragments were amplified by eight PCR cycles for library preparation. Primer dimers and long DNA fragments were removed by AMPure XP beads purification step. DNA concentration was measured by Qubit fluorometric assay and library quality was determined by Bioanalyzer. The library was sequenced in Nextseq 500 76bp paired-end configuration using Illumina platform.
GEO
GSM4989572