Soil metagenome of farmland
The sequencing library is prepared by purified 16S rRNA gene amplicon, followed by 5' and 3' adapter ligation. Alternatively, "tagmentation" combines the fragmentation and ligation reactions into a single step that greatly increases the efficiency of the library preparation process. Adapter-ligated fragments are then PCR amplified and gel purified. The library construction and sequencing was conducted by Macrogen (Seoul, South Korea) following the manufacturer's protocol of illumina.
SUB9740705
1A_L1