GSM4593910: Sample201_wt; Mus musculus; RNA-Seq
Intestines of mice were removed, cleaned from feces and Peyer's patches and cut into small pieces. The intestinal pieces were transferred into a conical flask containing pre-warmed 1mM EDTA PBS. Intestinal epithelial cells were separated from the underlying tissue by incubation in 30 mM EDTA in Ca2+- and Mg2+-free PBS for 10 min at 37°C followed by vigorous shaking. Cells were harvested by centrifugation, washed, and multicellular epithelial aggregates were purified by repeated differential sedimentation at 1g in ice-cold PBS from contaminating cells and residual tissue. Total RNA was then extracted using the TRIfast reagent (Peqlab) according to the manufacturer's instructions. Profiling of whole-genome transcriptomic patterns was performed with 1000ng high quality total RNA per sample. Libraries of the samples were generated using the Illumina stranded mRNA library kit according to the manufacturer's instructions.
GEO
GSM4593910