GSM5344615: MOLM13_polysomalRNA_sgLUC_14d_rep4; Homo sapiens; RNA-Seq
For RNA isolation total RNA lysates or polysomal fractions were purified using Trizol-reagent and subsequent RNA purification according to the manufacturers instructions. Poly-A tail selection and library preparation were performed using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA). Sequencing was done using the Illumina Next Gen Sequencing HiSeq platform (Illumina, San Diego, CA) with 150bp, paired-end reads.
GEO
GSM5344615