16S rDNA-Seq: forest soil
The total community DNA was extracted from soils by use of a MoBio Power Soil DNA extraction kit. The V4每V5 region of the 16S rRNA gene was amplified in triplicate using the barcoded 515F and 907R primer set. PCR products were quantified, purified and sequenced by Illumina Miseq platform for assessment of bacterial diversity and community structure.
SUB1609495
WB_3