RNA-seq of liver: adult male Rattus norvegicus
Total RNA was extracted from the tissue using TRIzol Reagent according the manufacturers instructions (Invitrogen) and genomic DNA was removed using DNase I (TaKara). Then RNA quality was determined by 2100 Bioanalyser (Agilent) and quantified using the ND-2000 (NanoDrop Technologies). Only high-quality RNA sample was used to construct sequencing library. Illumina Hiseq xten/Nova seq 6000 Sequencing and reading were finished based on published method
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