Genomic sequence of Matsucoccus macrocicatrices: immature cyst
Libraries were prepared using the Kapa Hyper Prep Kit KR0961-v1.14 (Kapa Biosystems, Wilmington, MA, USA) following the manufacturers instructions with the following modifications: (1) half reaction volumes were used, (2) universal iTru y-yoke adapters were ligated onto the genomic DNA, and (3) iTru5 and iTru7 primers were used to produce Illumina TruSeqHT compatible libraries (Glenn et al. 2016). Sera-Mag Speedbeads (Thermo-Scientific, Waltham, MA, USA) were used for purification. A total of 3,998,7582 reads were paired by name in Geneious 10.0.9 (Biomatters, Auckland, New Zealand)
SUB4469879
MM-19 seq