GSM5344592: MOLM13-Cas9_empty-vector-control_RNAseq_rep1; Homo sapiens; RNA-Seq
For RNA isolation from cell were FACS sorted for high RFP-expressing cells (guide expressing cells), 0.5x10^6 cells were lysed in 350 µl of RLT buffer and RNA was isolated using the RNeasy Mini Kit (Qiagen) according to manufacturers instructions for cell ine experiments. Poly-A tail selection and library preparation were performed using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA). Sequencing was done using the Illumina Next Gen Sequencing NextSeq platform (Illumina, San Diego, CA) with 37bp, paired-end reads.
GEO
GSM5344592