GSM2101537: rps0b-Null YPAD Ribo-Seq rep2, sigma1278b deletion collection background; Saccharomyces cerevisiae Sigma1278b; OTHER
Cells were lysed by vortexing with glass beads in lysis buffer. Lysates were clarified by centrifugation at 16k xg. RNAse digestion of lysate and isolation of ribosomal footprints (Ribo-Seq) or random fragmentation and size selection (26-40 nt) of polyA-selected total RNA (RNA-Seq) was followed by 3' adapter ligation, reverse transcription, circularization of the RT product and PCR amplification.
GEO
GSM2101537