GSM4942451: IGF2BP2_IP_1; Mus musculus; RIP-Seq
The beads were washed 6 times with lysis buffer and then divided into two groups, one for RNA isolation from IGF2BP2-RNA complexes and another for the western blotting assay for IGF2BP2 immunoprecipitation.The IGF2BP2-bound RNAs were isolated from the immunoprecipitate of monoclonal Flag antibody (Sigma), followed by the preparation of the Illumina Truseq pair-end libraries. The reverse transcription was performed with RT primers harboring 3′ adaptor sequence and random hexamer. The generated cDNAs were PCR-amplified and the 200–500 bp products were purified
GEO
GSM4942451