GSM2974026: C2G2Res1_R3; Mus musculus; ChIP-Seq
brains were extracted, snapfrozen in isopentane, subsequently the hippocampus was dissected,hippocampi of both hemispheres were powderised at - 80 ͦC, blended and split in two equal portions. the portion used for the epigenetic investigation was further split in two equal portions of which one was used for the ChIP experiments. For chromatin immunoprecipitation, targeting H3K4me3, DNA was fixated, sonicated, followed by antibody based capture and several washings. Following decrosslinking,  DNA was extracted from one portion, using regular phenol/chlorophorm based extraction protocol as published in (Schraut et al. 2014) library preparation was performed by Nxt-Dx (Ghent, Belgium), using the NEBNext Ultra II DNA Library prep kit for Illumina (NEB, Ipswitch, Massachusetts, USA).
GEO
GSM2974026