GSM5342461: 5 samples: tag; Mus musculus; RNA-Seq
Cells were determined by FlowCount beads (Beckman Coulter) using combination of Biotin-CD9 and Biotin-CD63 (Biolegend) conjunction with TotalSeq™-A095(1-5) PE Streptavidin, respectively For scRNA-seq, cDNA libraries and hashtag libraries were prepared combined by an BD Rhapsody system and an optimized process similar to the magnetic bead-based bulk RNA-seq method (Shichino et al. JCI insight 2019). Sequencing libraries were generated by using NEBNext UltraII FS library prep kit (New England Biolabs), and QC of cDNA/Hashtag libraries was performed by MultiNA (Shimazu) and qPCR library quantification assay (KAPA). BD Rhapsody WTA
GEO
GSM5342461