RNA-seq of Euglena gracilis: photoautotrophic growth at 21C in E. gracilis media with 5 uM HgNO3; sampled during mid exponential growth phase (mercury stress group)
Total RNA was isolated using TRIzol and treated with DNase I; Poly(A) mRNA was enriched using oligo dT-beads and cDNA libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina.
SUB7904483
Hg_1