Satellite Cells, Cripto High, Biological Replicate 1
GFP+ CriptoHigh and CriptoLow SCs were sorted from injured hind limb muscles of Tg:Pax7-nGFP mice, at 3 dpi. FACS-sorting was performed on cell sorter FACS Aria II (BD Biosciences). Pooled GFP+ SCs from 4 individual mice were analysed by RNA-seq. Total RNAs from sorted cells, were extracted in TriZol (Sigma-Aldrich), by using Direct-Zol Miniprep kit (Zimo-Research) following the manufacturers instructions. Stranded total RNA (2x20M) library was prepared for the Illumina NextSeq500 platform (TruSeq stranded total RNA with Ribo-Zero) and paired-end 2x75 cycles, ~40,000,000 reads/sample sequencing has been performed
SUB7553795
SC_CriptoHigh_rep1