Personalized Prescription of Imatinib in Recurrent Granulosa Cell Tumor of the Ovary: Case Report.
DNA was extracted from the FFPE tissue using the AnaPrep FFPE DNA extraction kit following the manufactural instruction. Whole-exome DNA was captured from total genomic DNA using the SeqCap EZ System from NimbleGen according to the manufacturers instructions. Briefly, genomic DNA was sheared, size selected to roughly 200-250 base pairs, and the ends were repaired and ligated to specific adapters and multiplexing indexes. Fragments were then incubated with SeqCap biotinylated DNA baits followed by the LM-PCR, and the RNA-DNA hybrids were purified using streptavidin-coated magnetic beads. The RNA baits were then digested to release the targeted DNA fragments, followed by a brief amplification of 15 or less PCR cycles.
SUB4723697
GCT