GSM4593963: SW480 Time 0 Rep 1; Homo sapiens; RNA-Seq
RNA was extracted using the RNeasy kit from Qiagen. The optional DNAse incubation was performed. Ribosomal RNA (rRNA) was removed using biotinylated, target-specific oliges combined with Ribo-Zero rRNA removal beads according to the Illumina total RNA sequencing protocol. The RNA was fragmented and copied into first strand cDNA using reverse transcriptase and random primers, followed by second strand cDNA synthesis using DNA Polymerase I and RNase H. The double-stranded cDNA was used as input to a standard Illumina library prep with end-repair, adapter ligation, and PCR.
GEO
GSM4593963