GSM1571307: Grouper_spleen; Epinephelus coioides; miRNA-Seq
After growth for 48 h, the GS cells were harvested and total RNA was extracted using TRIzol reagent,with a little modification. After addition of isopropanol, the RNA extract was incubated at -20°C  for 2 h to acquire more low-molecular-weight RNAs. After electrophoresing on 15% polyacrylamide–8 M urea gel, the small RNAs with about 30 nt were extracted, and a pair of Solexa proprietary adaptors as PCR primer was ligated to both 5′ and 3′ ends of the RNAs. After reverse transcription reaction from the RNAs, the resulting cDNA was amplified to produce sequencing libraries.
GEO
GSM1571307