GSM1214277: PNST Twist1; Mus musculus; ChIP-Seq
For ChIP experiments, ECCs, limb buds, and PNST cells were cross-linked and isolated according to manufacturer’s protocols (Magnify ChIP kit, Invitrogen).  Crosslinked DNA complexes were sheared by sonication (Virsonic 60, Virtis, output of 5) 6 times for 5 seconds with a 2-minute refractory period on ice.  Samples were sheared to a size of 150-250 basepairs (bp). ChIP was then performed according to the manufacturer’s protocol (Magnify ChIP Kit, Invitrogen). A single read genomic DNA library was generated using an IntegenX robot (Apollo 324) and PrepX DNA library kit (IntegenX 400040) was utilized according to manufacturer's protocols on mouse genomic DNA.
GEO
GSM1214277