GSM2630115: R+ WT 0030; Drosophila melanogaster; OTHER
Genomic DNA was subjected to IP with 4μg of mouse monoclonal S9.6 antibody. Immunoprecipitated DNA was eluted and purified using standard protocols. 10 ng DNA, quantified by Qubit dsDNA HS Assay Kit (Invitrogen) was used for library preparation. Endrepair, adenylation, ligation of adapters and PCR enrichment for 15 cycles was performed using TruSeqRNA Sample Prep Kit (Illumina) according to manufacturer’s recommendations. Purified libraries were quantified by Qubit dsDNA HS Assay Kit (Invitrogen) and size distribution was evaluated using Bioanalyzer DNA 1000 assay (Agilent).
GEO
GSM2630115