Borrelia burgdorferi sensu stricto Europe genome sequencing
DNA samples were prepared according to Nextera DNA sample preparation guide (Qiagen). The samples were diluted to 50ng/μl concentration and “tagmented” by simultaneously fragmenting DNA using transposomes and adding adapters. After tagmentation samples having adapters on both ends underwent 5 PCR cycles to amplify the product. The sequences were indexed and the resulting libraries were validated using Agilent 2100 Bioanalyser.  Mate-paired (MP) libraries were generated using an Illumina Nextera Mate Pair Library Preparation Kit. After circularization samples were fragmented using a Covaris instrument. Adapter and index sequences were ligated to the fragments according to the manufacturer’s recommendation. Samples having adapters on both ends underwent 5 PCR cycles to amplify the product. The resulting libraries were validated using Agilent Tapestation 2200.
Bavarian Health and Food Safety Authority
Bbss_PAli