GSM5170125: micu-1;mcu-1_2 [8264]; Caenorhabditis elegans; RNA-Seq
worms were collected, pelleted and dissolved in 1mL Trizol. Total RNA was extracted using the miRNeasy Micro kit (Qiagen) according to manufacturer's protocol. cDNA libraries were prepared from 5 ng total RNA using the SMART-seq2 protocol and tagmented with the Nextera XT kit (Illumina). Library purification and size selection was carried out with AMPure XP beads (Beckman-Coulter) and final library size distribution was measured via High Sensitivity D5000 assay on a TapeStation 4200 System (Agilent). Library concentration was determined using the HS dsDNA assay on a Qubit 3.
GEO
GSM5170125