GSM1571853: input CBP-gp; Drosophila melanogaster; ChIP-Seq
After fixation, the cells were washed and sonicated using a Bioruptor (Diagenode) to generate chromatin extract. Chromatin extract was cleared by centrifugation and used in ChIP with affinity-purified anti-Drosophila CBP antibodies, pre-bound to a mix of Protein A and G Dynabeads. CBP bound DNA was purified, and sent to Uppsala Genome Center for SOLiD (TM) ChIP-Seq Library preparation (Rev date 18 March 2010), size selection (100-150 bp + adapters 90bp ≈250 bp), and SOLiD sequencing. Libraries were prepared for sequencing using standard SOLiD protocols
GEO
GSM1571853