GSM4718895: IP:H3K27ac AID-METTL3:-METTL14 dd (noIAA) Rep1; Mus musculus; OTHER
Cells were immobalised to concavalin beads, permeabilised with digitonin and incubated with antibody. After washing, the cells are incubated with A-Mnase protein to digest around the protein-bound DNA. Upon diffussion of DNA-protein complexes out of the cells DNA was collected and size selected with SPRI beads. We used 10ng DNA input for library preparation using Accel-NGS 2S Plus DNA Library Kit (Swift) according to the manufacturer's protocol. Sequencing was performed in 50pb paired-end reads using Novaseq 6000.
GEO
GSM4718895