GSM3290768: FPS16: thrA_A2_F1_I0_R2; Escherichia coli BW25113; RNA-Seq
Total RNA was isolated and purified using Qiagen's RNeasy minikit column according to the manufacturer's specifications. The total RNA quality was checked with the Agilent Bioanalyzer RNA 600 nano kit. Ribosomal RNA (rRNA) was removed utilizing Ribo-Zero rRNA removal kit (Epicentre) for Gram-negative bacteria. The KAPA RNA HyperPrep kit (Kapa Biosystems) was used for generation of paired-end, strand-specific RNA sequencing libraries. The final pool of libraries was subjected to a 1x SPRI bead size selection to further remove unincorporated PCR primers. RNAsequencing libraries were run on an Illumina NextSeq 550 machine using a NextSeq 500/550 Mid Output v2 kit (150 cycles).
GEO
GSM3290768