Illumina HiSeq 2500 paired end sequencing; TSLR sequencing of Landsort Deep 3.0µm filter fraction
TSLR metagenomic sequencing of Landsort Deep in the Baltic Sea, 3.0µm filter fraction
Environmental DNA (eDNA) was isolated from the impact filters and                 library construction was performed according to Rusch, et al. 2007. Briefly, shipboard frozen                 filters contained in the following storage buffer: 1X Tris-EDTA (TE) buffer (Invitrogen), 50mM                 EGTA and 50mM EDTA, were thawed to room temperature and aseptically cut for DNA extraction.                  Filter fragments were placed in 50ml conical tubes and lysis was performed as previously described.                  DNA was ultimately purified using a phenol/chloroform extraction method followed by ethanol                  precipitation. Libraries were constructed using the Illumina TruSeq Synthetic Long-Read DNA Library prep kit.
J CRAIG VENTER INSTITUTE
jcvi_lsd_678_30_tslr