Melanaphis sacchari genomic sequencing project
To construct a genomic DNA library (400 bp target reads) for the sugarcane aphid, Melanaphis sacchari, genomic DNA (1 µg) was digested for 4 min to obtain the desired fragment length using the Ion Xpress™ Plus gDNA Fragment Library Preparation kit (Life Technologies, Carlsbad, CA). After library preparation, the library was quantified using the Ion Library Quantitation Kit (Life Technologies). The quantified library was diluted to 20 pM for template preparation using the Ion PGM™ Template OT2 400 Kit (Life Technologies). The prepared library was loaded onto an Ion 318™ Chip v2 (Life Technologies) and sequenced on the Ion PGM™ System (Life Technologies) using the Ion PGM™ Sequencing 400 Kit (Life Technologies).
USDA-ARS
Sugarcane Aphid