GSM4718292: H3K9me3_ChIP-seq_LLC_KO_rep1; Mus musculus; ChIP-Seq
Ten million cells per sample were cross-linked with 1% formaldehyde for 15 min, quenched with glycine, and subjected to two-step cell and nuclear lysis in the presence of protease inhibitors. Shearing of samples were optimized such that the final DNA length was approximately 400 base pairs using a Branson sonifier, and was followed by incubation with anti-H3K9me3 polyclonal antibody (Abcam, ab8898) overnight at 4˚C. Immunoprecipitation was performed with 50:50 mixes of magnetic dynabeads Protein A and G (Invitrogen). End-repair, A-tailing, adapter ligation, and library amplification were performed with the Kapa HyperPrep kit (Roche).
GEO
GSM4718292