GSM1576405: HD30_NEU_3_SL27215; Homo sapiens; RNA-Seq
PBMC and PMN were fractionated from fresh human blood via ficoll separation. CD3+ T cells, CD11c+ mDC, CD14+ monocytes, CD15+ Neutrophils, CD19+ B cells and CD56+ NK cells were isolated from PBMC/PMN by MACS enrichment followed by FACS sorting. Total RNA was extracted from PBMC and sorted immune cells (≤0.5x106 cells) from the two TIV-vaccinated subjects using the automated Maxwell 16® magnetic particle processor and a Maxwell 16 LEV simply RNA kit (Promega Corp.). Polyadenylated RNAs were isolated using NEBNext magnetic oligo d(T)25 beads. NEBNext mRNA Library Prep Reagent Set for Illumina (New England BioLabs Inc.) was used to prepare individually bar-coded next generation sequencing expression libraries. 50bp, paired end RNA-seq (25 million read)
GEO
GSM1576405