Dna-seq of homo sapien stool
Both ITS2 region and the 16Sv4 hypervariable region were amplified by PCR and sequenced in the MiSeq platform (Illumina). Specific primers were used for DNA amplification containing adapters for sequencing and single-index barcodes so that the PCR products may be pooled and sequenced directly, targeting at least 10,000 reads per sample. The 2x300 bp and the 2x250 bp paired-end protocols were used for ITS2 (fungi) and 16Sv4 (bacteria) detection, respectively.
SUB5216284
Cmmr-a83v7-283