16S rRNA amplicon sequencing of extraction control
The V4 region of the 16S rRNA gene was amplified by using primers 515F and 805R targeting both bacteria and archaea. 25 amplification cycles were employed. Sequencing with 2 x 300 nt was performed using the V3 MiSeq kit on a MiSeq platform (Illumina). Amplification and sequencing was performed by the Groupe Interdisciplinaire de Génoprotéomique Appliquée (GIGA; Liège, Belgium).
SUB2679173
Ctrl_MV1_16S