GSM2801339: Set1rep1; Saccharomyces cerevisiae; OTHER
The detailed extract protocol is described in the experimental procedures of the article. PTH-Set1 was isolated by three purification steps, two of which in denaturing conditions. The RNA crosslinked to Set1 was submitted to adaptors ligation, isolated and reverse transcribed. Between half to ¾ of the cDNA solution was used to performed multiple PCR reactions in individual 25 µl reactions containing 1/10 of the whole cDNA reaction for 13 cycles under standard conditions. The PCR reactions were pooled and treated with 200 U of Exonuclease I (NEB)/ml   for 1 hour at 37°C. DNA was purified on PCR clean up columns (NucleoSpin® Gel and PCR Clean-up, Macherey-Nagel) and sequenced using  Illumina technology (NextSeq 500).
GEO
GSM2801339