Polypterus senegalus Raw sequence reads, non-regenerating fin (NRF)
Juvenile bichirs (Polypterus senegalus) were obtained from local pet traders and maintained in accordance to animal care guidelines approved by the Animal Care Committees at the Universidade Federal do Para (protocol number 037-2015). A total of 12 Polypterus specimens ranging 5-8 cm were used in the study. Animals were kept in individual tanks with regular water changes and fed once a day. For fin regeneration studies, fish were anesthetized in 0.1% MS-222 (Sigma) and pectoral fins were bilaterally amputated across the endoskeleton. Fin endoskeleton tissue was sampled and labelled as NRF tissue, and regenerating fin tissue was sampled from left and right pectoral fins at 1, 5 and 9 dpa. All tissue collected was stored in RNAlater (Sigma) for RNA extraction and subsequent qPCR or RNA-seq experiments.
SUB4291643
polypterus_NRF_PS3