GSM4593106: CaPS_EB10_IgG_CUT&RUN_group2; Mus musculus; OTHER
CUT&RUN was performed as previously described in Skene et al., 2018 (Nat. Protoc.), in native conditions and with modifications for high-calcium/low-salt condititions as described in Meers et al., 2019 (eLife). Libraries were prepared as described in Bowman et al., 2013 (BMC Genomics). In brief, this protocol includes end repair, A-tailing, adapter ligation, and real-time PCR amplification, with SPRI cleanup of samples between each step.
GEO
GSM4593106