GSM2802586: WT1 CD4+YFP+; Mus musculus; RNA-Seq
RNA was extracted using Qiagen AllPrep DNA/RNA kit with an additional on-column Dnase 1 treatment. Each sample was processed using Truseq Stranded mRNA Sample Preparation, October 2013, Rev. E., with one modification at the end of the protocol on pg.41; post-amplification the libraries were purified with Ampure XP beads twice. The libraries were individually barcoded and multiplexed into a single sample. This multiplexed sample was clustered on a Paired-end, RAPID, 2-lane flowcell and sequenced on a Hiseq 2500 DNA sequencer for 100 cycles in each read direction, for a total of 200 cycles, plus an additional 7 cycles to sequence the barcodes. 
GEO
GSM2802586