GSM5456696: Hypoxia_input; Homo sapiens; RNA-Seq
Total RNA was extracted from tissues using TRIzol reagent (Invitrogen,15596018), followed by DNase I (NEB, M0303L) treatment to remove DNA contamination. Additional phenol-chloroform isolation and ethanol precipitation treatment was performed to remove enzyme contamination. Total RNA was fragmented into ~130-nucleotide-long fragments by magnesium RNA fragmentation buffer (NEB, E6150S). The fragmentation was stopped by adding RNA fragmentation stop solution followed by ethanol precipitation. 8ng of fragmented total RNA was used as input and remained RNA was used to do the m6A-seq. The libraries were sequenced on Illumina Hiseq X10 with paired-end 150 bp read length.
GEO
GSM5456696