Genome sequencing of WT strain
Strains were grown for 18 h in tryptone soya broth (TSB, Oxoid) at 25°C with 50 µg/mL kanamycin sulphate added to the media of mutant strains. Genomic DNA was isolated with the DNeasy Blood and Tissue Kit (QIAGEN) according the manufacturer's protocol for Gram-negative bacteria. Library preparation and genome sequencing was carried out using MiniSeq High Output kits with 150 cycles and the Illumina MiniSeq System (Illumina).
CSIRO Agriculture and Food
WT