The WNT targets in hESCs-derived hindgut cultures are composed of multi-lineage genes. [Endoderm RNA-seq and ChIP-seq data sets]
H9 embryonic stem cells were differentiated into definitive endoderm and then treated with FGF4/WNT3a in order to generate intestinal organoids as previously described by Spence, et al. Nature 470:105-109(2010) and McCracken, et al. Nature Protocols 6:1920-1928 (2011). We performed the ChIP experiment with the kit (Millipore, Bedford, MA) according to the manufacturer’s protocol. The cells were sonicated by the Diagenode Bioruptor and chromatin was sheared to an average length of 300-500bp. The lysates were incubated with a monoclonal antibody against TCF7L2 or a polyclonal antibody against TCF7L2, respectively. Each experiment was replicated twice. ChIP and input DNA were amplified using the illumina ChIP-seq DNA Sample Prep Kit.
institute of zoology
TCF7L2 targets_polyclonal-2