VSG-seq of Trypanosoma brucei brucei: Sample d18
50 - 100 µl blood was collected by submandibular bleed from Balb/cByJ mice         infected intraperitoneally with ~5 pleomorphic EATRO1125 parasites. RNA was isolated from         blood sample, cDNA was made by VSG-specific 1st strand synthesis, and VSG PCR was performed         to enrich for VSG cDNA. VSG amplicon libraries were made using NexteraXT sample prep         kit.
Rockefeller University
Mouse_4_day_18