total RNA: EBOV infected Huh7 cells
EBOV-infected cells were harvested 1 day post infection. Poly(A)+ RNA was isolated from the total RNA samples and fragmented with ultrasound (2 pulses of 30 sec at 4C). First-strand cDNA synthesis was primed with a N6 randomized primer. Then, the Illumina TruSeq sequencing adapters were ligated to the 5' and 3' ends of the cDNA. The cDNA was finally amplified with PCR using a proof reading enzyme. For Illumina sequencing, the cDNAs in the size range of 400 - 600 bp were eluted from preparative agarose gels.
SUB9183141
ebov_inf_total_T1757_S7