16S rRNA gene amplicons of linuron/chlorpropham/chlortoluron enrichment culture initiated with biofilter material
To generate 16S rRNA gene amplicons for sequencing, a PCR was performed targeting either the V4 region of the 16S rRNA gene using primers A519F and 802R (Klindworth et al., 2013) or V3-V4 region using primers Bakt_341F and Bakt_805R (Klindworth et al., 2013) amended with overhang adapter sequences. The amplicons were obtained and purified according to the Illumina 16S Metagenomic Sequencing Library Preparation protocol (Illumina, San Diego, CA).
SUB9311350
EC_BPS-_CLP_t1_1