GSM2802533: WT rep2 (mTAIL-seq); Caenorhabditis elegans; RNA-Seq
RNA was extracted with Trizol and DNase treated. A splint oligo was used to favor poly(A)+ RNAs being ligated to a 3′ adapter. After partially digesting the RNA from the ligation reaction with RNAse T1, RNAs were pulled down using Streptavidin beads, then 5' phosphorylated by PNK reaction. RNA was eluted and size selected for 250-1000 nt fragments. This was done by gel extraction and purification from a 6% Urea-PAGE gel. Libraries were normalized, pooled and then sequenced in the Illumina MiSeq platform (51 x 251 bp paired end run) with PhiX control library and the spike-in controls mixture. The quantified fluorescent signals were saved and processed by tailseeker2.
GEO
GSM2802533