GSM3454197: HG001_DrsaI_Comp_TotRNA_R3; Staphylococcus aureus; RNA-Seq
Bacterial lysis was performed with FastPrep and the RNA purification followed strictly the procedure described for the FastRNA Pro Blue Kit (MP Biomedicals). DNase I (0.1 U/ml) treatment was performed 1h at 37°C. The reactions mixtures were then purified by phenol/chloroform/isoamylalcohol and subsequent ethanol precipitation. RNA pellets were re-suspended in sterile milliQ water. RNA quality and quantity assessments were performed on Agilent Nano Chip on the Bioanalyzer 2100. RNAs were treated to deplete abundant rRNAs, and the cDNA libraries were performed using the ScriptSeq complete kit (bacteria) from Illumina. The libraries were sequenced using Illumina MiSeq with a V3 Reagent kit (Illumina), which preserves the information about the orientation of the transcripts and produces reads of 150 nts, which map on the complementary strand. Each RNA-seq was performed in triplicates.
GEO
GSM3454197