GSM2103967: Infected; Ocimum basilicum; Peronospora belbahrii; RNA-Seq
Total RNAs were extracted from leaves of healthy and infected basil plants using Trizol reagent (Thermo Fisher Scientific, MA) following manufacturer’s protocol. After removal of genomic DNA by DNase I (New England Biolabs, MA) treatment, RNA samples were quantified using NanoDrop 1000 (Thermo Fisher Scientific, MA) and assessed for integrity using Agilent Bioanalyzer 2100 (Agilent, MA). Library construction was conducted using Illumina TruSeq mRNA library preparation kit (Illumina, CA)
GEO
GSM2103967