Viral metagenomics of anal swab samples from wild and zoo birds in China: Long-tailedRosefinch03
From2016to2018,toinvestigatetheviromeofwildbirds,morethan4,000cloacalswabswerecollectedfrom8differentprovincesinChina.Allsampleswerecollectedbydisposablematerials.Eachsamplewasre-suspendedintenvolumesofphosphate-bufferedsalineandvigorouslyvortexedfor5min.Thesupernatantswerethencollectedaftercentrifugation.500lofeachsupernatantwasfilteredthrougha0.45mfiltertoremoveeukaryoticandbacterialcellsizedparticles.ThefiltratesenrichedinviralparticlesweretreatedwithDNaseandRNasetodigestunprotectednucleicacidfor60min.RemainingtotalnucleicacidwasthenisolatedusingQIAampMinEluteVirusSpinKitaccordingtomanufacturerprotocol.Morethan300libraries(eachlibrarycontainedpooledsamples)werethenconstructedusingNexteraXTDNASamplePreparationKitandsequencedusingtheIlluminaHiseq2500orNovaSeq6000platformwith250basespairedendswithdualbarcodingforeachlibrary.Forbioinformaticsanalysis,pairedendreadsof250bpgeneratedby IlluminaMiSeqor NovaSeq platform  weredebarcodedusingvendorsoftwarefromIllumina.
SUB8801927
Long-tailedRosefinch03