454 secs of human gut microbiota from an equol producing woman
Bacterial DNA isolated from feces from an equol producing woman after receiving a daily treatment with isoflavone (80 mg) during six months. 16S rRNA genes were PCR-amplified using the universal primers Y1 (5?-TGGCTCAGGACGAACGCTGGCGGC-3?) (position 20-43 on the 16S rRNA gene of Escherichia coli) and Y2 (5?-CCTACTGCTGCCTCCCGTAGGAGT-3?)(positions 361-338) (Young et al., 1991). These primers amplify a 348 bp stretch of the prokaryotic rDNA embracing the V1 and V2 hypervariable regions. An amplicon library was prepared for pyrosequencing by mixing equal amounts of amplicons from the eight different samples (i.e., T0-T6 for samples treated and non treated with EMA). Pooled amplicons were then sequenced using a 1/8 picotitre plate in a 454 Titanium Genome Sequencer (Roche, Indianapolis, IN, USA).Sequences uploaded for this sample have been demultiplexed with the corresponding barcode, primers and adaptors have also been also removed.
SUB3558158
Month 6