GSM3453248: Keratinocyte_rep1 [ATAC-seq]; Homo sapiens; ATAC-seq
After defreezing, viability and cell number were quantified with Countes (Invitrogen), and nuclei were extracted from approximately 25,000 live cells with CSK buffer (10 mM PIPES pH 6.8, 100 mM NaCl, 300 mM sucrose, 3 mM MgCl2, 0.1% Triton X-100) on ice for 5 min. Transposase reaction was then carried out. Illumina adaptors were introduced by 9 to 12 total cycles PCR reaction. Amplified DNA fragments were then purified with QIAGEN MinElute PCR Purification Kit and size selected twice with Agencourt AMPure XP (1:1.5 and 1: 0.5 sample to beads; Beckman Coulter, Fullerton, CA, USA).
GEO
GSM3453248