GSM5077620: MADS27_ChIP-seq_Rep1; Oryza sativa; ChIP-Seq
Chromatin from the lysed nuclei was sheared using ultrasonicator (Covaris) at 4 degrees till the DNA fragments accumulate substantially in the size range of 200-500bp. The sheared cromatin was diluted ten times using ChIP dilution buffer (Song et al., 2016) and incubated overnight at 4oC with ~50ul of pre-equilibriated Myc-Trap Magnetic Agarose (Chromotek, ytma10). Washes, elution, de-crosslinking and DNA purification was done as described. The purified DNA was taken for library preparation. The ChIP library was constructed using NEBNext® Ultra™ II DNA Library Prep with Sample Purification Beads (Catalog no-E7103L) as per manufacturer's instructions. The adapter sequence used is AGATCGGAAGAGCACACGTCTGAACTCCAGTCA. The sequencing was done in single end mode in a Illumina HiSeq2500 platform.
GEO
GSM5077620