Transcriptomic comparisons of seven Vitis vinifera cultivars during the later stages of maturity: Sample CF.22
"Vitis vinifera L. cultivars Cabernet Franc, Cabernet Sauvignon, Merlot, Pinot Noir, Chardonnay, Sauvignon Blanc, and Semillon were grown at the University of Nevada, Reno's Experimental Vineyard. Each cultivar was surveyed over the course of several weeks in September and October 2012, depending upon the berry maturity of each cultivar. Berry clusters were collected between 11.00 h and 13.00 h. Sampling of replicate ?Brix levels was done on separate days for each cultivar and Brix level. Soluble sugars (?Brix) were measured to assess maturity with a digital refractometer (HI 96811, Hanna Instruments, Woonsocket, RI, USA). Individual berries were measured and fractionated based upon their ?Brix level in 1? increments from 19 - 26? Brix. Skins were then immediately separated from pulp by squeezing and dabbing expelled pulp onto a lab wipe and flash frozen with liquid nitrogen.    Frozen skin tissue was ground thoroughly in Retsch mixer mill (Retsch MM 301, Newtown, PA, USA). Total RNA was extracted with a modified CTAB extraction, followed by an on column DNase digestion treatment according manufactures instructions (Qiagen Mini Kit). RNA quality and quantity were assessed with RiboGreen and an Agilent 2100 Bioanalyzer and RNA LabChip assays according to manufactures instructions.    Library preparation and sequencing was performed by UCLA Neuroscience Genomics Core (UNGC). Sequencing libraries were produced from Total RNA using the TruSeq RNA library prep protocol (Illumina) according to manufactures instructions. TruSeq Version 3.0 sequencing chemistry (Illumina) and HiSeq TruSeq Flowcells were used for sequencing on an Illumina HiSeq 200 according to manufactures instructions.    The goal of this research project was to profile transcriptional changes in the mature berry skins as soluble sugars increase from 20 to 26 ?Brix, and to identify major players that affect fruit and ultimately wine quality."
University of Nevada, Reno
CF1-22