GSM3453144: SC_KM02 [Single Cell RNA-seq]; Mus musculus; RNA-Seq
Cells were sorted into 5µl Vapor-Lock (Qiagen) containing a droplet with primers and dNTPs. Cells were lysed at 65 degrees Celsius for 5 minutes. mRNA was then reverse transcribed by adding RT mix. RNA was processed by SORT-Seq, an automated version of the previously described Cel-Seq2 technique. Libraries were sequenced on a Illumina NextSeq500 using 75bp paired end sequencing.
GEO
GSM3453144