GSM1210920: br_ChIP-seq replicate1; Drosophila melanogaster; ChIP-Seq
Chromatin lysates were clarified from homogenized and sonicated tissues; protein-DNA complexes were isolated with antibody.  DNA fragments recovered following chromatin IP prepared for Illumina sequencing using the Epicentre Nextera DNA Sample Prep Kit (Cat. # GA0911).  Briefly, up to 12.5 ng DNA was included in the high molecular weight (HMW) tagmentation reaction (5 minutes at 55 degrees Celsius). Tagmented DNA was purified using a Qiagen MinElute column. Addition of barcoded PCR-compatible sites and library enrichment were performed using 12 cycles of PCR. Amplified DNA was purified using the Qiagen MinElute PCR Purification Kit. Library fragments of approximately 225 bp were gel purified and captured on an Illumina flow cell for cluster generation. Libraries were sequenced on a HiSeq2000 according to the manufacturer’s protocol. Libraries were prepared according to ChIP-seq library kit instruction Nextera DNA prep kit is FC-121-103
GEO
GSM1210920