GSM2801539: 605pre6; Homo sapiens; RNA-Seq
Single cells were sorted into 96-well plates containing guanidine thiocyanate buffer (Qiagen) supplemented with 1% β-mercaptoethanol. RNA was extracted using Ampure RNAClean SPRI beads. RNA was converted to full-length cDNA using oligo(dT) priming and SMART template switching technology followed by 24 cycles of PCR preamplification of cDNA. We used the amplified cDNA to construct standard Illumina sequencing libraries Nextera XT library preparation kit. Samples were sequenced by Illumina NextSeq.
GEO
GSM2801539