GSM4399884: Liver; Mus musculus; RNA-Seq
Lung and lymph node cells were extracted from pooled suspensions from lung (digested) or lymph node from 4-12 female individual litter-mate mice Liver cells were extracted from pooled suspensions from liver from 3 female litter-mate mice Lung and lymph node cells were sorted by flow cytometry (using a BD FACSAria III) and gated for dump- CD4+ CD44+ I-Ab NP311-325+ expression. Liver cells were sorted by flow cytometry (using a BD FACSAria III) and gated for dump- CD4+ CD44+ GP66+ expression. The sorted cell suspension from each sample was loaded into a single well of a 10× Genomics Chromium Single Cell Controller. Single-cell capture and cDNA and library preparation were performed with a Single Cell 3' v2 Reagent Kit (10× Genomics) according to manufacturer's instructions.
GEO
GSM4399884