RADcap sequencing of Ligurotettix coquilletti: voucher E18-810
3RAD libraries were generated according to Bayona-Vsquez et al. (PeerJ, 2019). Primary restriction enzymes were EcoRI and PstI. Third enzyme (for cutting adapter dimers) was NsiI. We then captured a subset of RAD loci using myBaits (Arbor Biosciences) followng the RADcap method o Hoffberg et al. (Molecular Ecology, 2016). We targeted 10,097 loci inferred from pilot RADseq assemblies.
SUB9389997
E18-810