GSM4989606: B321_RNA-seq; Homo sapiens; RNA-Seq
Total RNA was isolated using the RNeasy Mini Kit (Qiagen, CA, USA) according to the manufacturer's protocol. The quality and integrity of the RNA were confirmed by agarose gel electrophoresis and ethidium bromide staining, followed by visual examination under ultraviolet light. The sequencing library was prepared using the TruSeq RNA Sample Preparation kit v2 (Illumina, CA, USA) according to the manufacturer's instructions. In brief, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads, fragmented, and converted into cDNA. Adapters were then ligated to the cDNA, and the fragments were amplified by PCR.
GEO
GSM4989606