GSM3897378: LSK_MIG_C; Mus musculus; RNA-Seq
Sorted GFP positive cells were harvested and frozen in TRIzol reagent. RNA was extracted using Phenol-Chloroform. After RiboGreen quantification and quality control by Agilent BioAnalyzer, 2ng total RNA with RNA integrity numbers ranging from 9.3 to 10 underwent amplification using the SMART-Seq v4 Ultra Low Input RNA Kit (Clonetech catalog # 63488), with 12 cycles of amplification.Subsequently, 10ng of amplified cDNA was used to prepare libraries with the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) using 8 cycles of PCR.
GEO
GSM3897378