GSM4593053: IACSF02; Homo sapiens; RNA-Seq
Total RNA was extracted from CD1c+ DC RA synovial fluid and RA peripheral blood and the quality of all RNA samples evaluated using an Agilent Bioanalyzer. RNA samples were reverse transcribed, and sequencing libraries were constructed using NuGen Ovation Universal RNA-Seq System (catalogue 0402-A01) according to the manufacturer's protocol. The resulting sequencing libraries were analyzed using the Caliper LabChip GX and quantified using KAPA qPCR. Libraries were then normalized and pooled in 1 batch of 6 and 1 batch of 5. Each pool was clustered and sequenced on an Illumina NextSeq500 instrument using 2 × 100 bp paired-end reads, following the manufacturer's protocols.
GEO
GSM4593053