GSM2102535: Cont4b RNA-seq; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol reagent from amnion membrane and the total RNA was then treated with DNaseI to eliminate residual DNA. The 500 ng of extracted RNA samples were subjected to directional RNA seq library preparation. We eliminated ribozomal-RNA (rRNA) from total RNA with RiboZero kit (Illumina)  before reverse transcription with the SuperScript III kit (Invitrogen) using oligo-d(T) and random hexamers. The second strand cDNA was synthesized with dUTP. After the second strand synthesis, the products were fragmented, end-repaired, dA tailed and added Illumina sequencing adapters. Before PCR amplification, we treated the product with Uracil-DNA glycosylase.
GEO
GSM2102535