Vibrio alginolyticus EPGS WT Tm37
The strains were grown overnight in LBS at 37°C. They were then diluted 1:100 in LBS, grown at 37°C and harvested after 9 h. Total RNA was isolated by RNA extraction kit (Tiangen, china). The RNA samples were digested with DNase (Promega, USA) to eliminate genomic DNA contaminant. The quality of RNA samples was assessed using NanoDrop ND-1000 spectrophotometer (Labtech, USA). The integrity of RNA was assessed using standard denaturing agarose gel electrophoresis. From each sample, 1 mg of total RNA was amplified and transcribed into fluorescent cDNA
Chinese National Human Genome Center at Shanghai
WT37