GSM3897331: Fgfr2_rep1; Mus musculus; RNA-Seq
Total RNA was isolated from each replicate using the mirVana isolation kit (Ambion, Life Technologies, Grand Island, NY). Total RNA samples with the RNA integrity number (RIN, Agilent 2100 Bioanalyzer) ≥8.0 were used to prepare a library of template molecules suitable for subsequent sequencing on an Illumina (St. Louis, MO) HiSeq platform. Polyadenylated RNA was purified from the total RNA samples using Oligo dT conjugated magnetic beads and prepared for single-end sequencing according to the Illumina TruSeq RNA Sample Preparation Kit v3. All the libraries were sequenced using the TruSeq SBS kit on an Illumina HiSeq 2000 RNA libraries were prepared for sequencing using standard Illumina protocols
GEO
GSM3897331