GSM4594074: 749ATX; Homo sapiens; RNA-Seq
Total RNA is extracted from vehicle or ATX-treated cells (n=4 per group) by standard Trizol method and quantified using the Qubit RNA BR assay (Denovix). Quality of RNA is assessed using the Agilent RNA Nano chips on a Bioanalyzer instrument. For all the samples, RNA integrity number (RIN) was >9. mRNA libraries are prepared from 200 ηg total RNA  using the TruSeq Stranded mRNA library preparation kit (Illumina Cat# 20020594) as per manufacturer's recommendations.  Individual libraries are quantified using a fluorometric high-sensitivity dsDNA assay (Denovix). Library quality is assessed on a high sensitivity DNA chip on the Agilent 2100 Bioanalyzer system (Agilent). Libraries are prepared for sequencing (volumetrically pooled, diluted and denatured): 75 paired end read-cycles on an Illumina NextSeq 550 sequencer.
GEO
GSM4594074