GSM5077490: K562_PROseq_DMSO; Homo sapiens; OTHER
Cells were permeabilized and run-on reaction was performed at 30°C for 5min with biotinylated-NTPs. Run-on reaction was halted with TRIzol reagent buffer and total RNA was extracted using chloroform/ethanol precipitation. Dynabeads M-280 streptavidin beads (cat#: 11206D) were used to isolate biotinylated RNA. RNA libraries were generated from isolated biotinylated RNA for sequencing using the NEBNext Ultra II Directional RNA Library Prep Kit (cat#: E7760L) protocol for Illumina sequencing.
GEO
GSM5077490