GSM4593859: K562 shSRA-3; Homo sapiens; RNA-Seq
Total RNA was extracted from the sorted cells and purified using QIAzol Lysis Reagent and miRNeasy Micro Kit (Qiagen). The RNA samples for sequencing were prepared according to Smart-seq2 method (Picelli et al., 2014) with some modifications as previous described (Hu et al., 2018). The RNA-seq libraries were prepared with an End-It DNA End-repair Kit (Epicenter #ER81050) and a Multiplexing Sample Preparation Oligonucleotide Kit (Illumina) and submitted for single-end sequencing on illumina Hiseq2500 as previous described (Hu et al., 2013).
GEO
GSM4593859