GSM3724846: HC2_F&IM&2dpi_R3; Solanum pimpinellifolium; RNA-Seq
During the collection, all inflorescences were immediately immersed in ice-cold RNAlater (QIAGEN) solution at five times the volume of the sample in order to prevent RNA degradation. After sample collection, vacuum infiltration was applied until tissues sunk to the bottom of RNAlater solution. The tissues were then stored in RNAlater at -80℃.  For the dissection, meristems and buds of different developmental stages were isolated by forceps under a dissection microscope and immediately put into a 1.5 ml eppendorf tube in 1 ml of fresh RNAlater that was kept on ice. The samples were stored at -80 ℃ prior to the RNA extraction.RNA extraction was conducted using Trizol® (Invitrogen Inc. USA) following manufacture's recommendation. Strand directed RNA-seq libraries of approximately 250 bp fragments were prepared following the protocol of Zhong et al., 2011.
GEO
GSM3724846