A31
After amplification of the purified and polymerase chain reaction, the DNA library was constructed. Then, the library was hybridized with the probe to capture the target region using an Agilent SureSelect XT custom kit . Dynabeads MyOneTM Streptavidin T1 magnetic beads were used to enrich the DNA belonging to the region of interest. The original library was amplified using a high-fidelity polymerase and purified by magnetic beads so it would be suitable for sequencing.
SUB9224063
A31