GSM2802852: VEH-4hr-1; Homo sapiens; RNA-Seq
RNA was extracted using RNAeasy kit (Qiagen). Intact poly (A) RNA was purified from total RNA samples (100-500 ng) with oligo(dT) magnetic beads sequencing libraries were prepared as described using the Illumina TruSeq RNA Library Preparation Kit v2 (RS-122-2001, RS-122-2002).  Purified libraries were qualified on an Agilent Technologies 2200 TapeStation using a D1000 ScreenTape assay (cat# 5067-5582 and 5067-5583).  The molarity of adapter-modified molecules was defined by quantitative PCR using the Kapa Biosystems Kapa Library Quant Kit (cat#KK4824).  Individual libraries were normalized to 10 nM and equal volumes were pooled in preparation for Illumina sequence analysis. Sequencing libraries (18 pM) were denatured chemically and applied to an Illumina TruSeq v3 single read flowcell using an Illumina cBot.  Using an Illumina TruSeq SR Cluster Kit v3-cBot-HS (GD-401-3001) hybridized molecules were clonally amplified and annealed to sequencing primers.   Following transfer of the flowcell to an Illumina HiSeq instrument (HCS v2.0.12 and RTA v1.17.21.3), a 50 cycle single read sequence run was performed using TruSeq SBS v3 sequencing reagents (FC-401-3002).
GEO
GSM2802852