Soil 16S rRNA amplicon sequencing
Sequencing was performed on the Ion PGM using the primers F515 and R806. PCR conditions: 95 C for 10 min, then 25 cycles at 95 C for 30 s, 57 C for 30 s, and 72 C for 1 min, followed by 72 C for 7 min.
SUB7071236
21LusakaDay52CM1