GSM1576709: gon_n2wt_jr01; Caenorhabditis elegans; RNA-Seq
Tissues were placed into 300µl TRIzol (Invitrogen) on ice.  60µl chloroform was added to the TRIzol/tissue samples and phases were separated using prespun Phase Lock Gel-Heavy tubes (5 Prime).  The aqueous phase was transferred to a new tube and mixed with 125-175µl isopropanol and 1µl GlycoBlue (Ambion).  RNA was precipitated overnight at  -20°C and pelleted at 13,000xg at 4°C for 30 minutes.  The pellet was washed with 75% ethanol, dried, and resuspended in 10-20µl ddH2O. Gonad samples were depleted of rRNA with a RiboZero kit (Epicentre) and whole worm samples were poly-A selected.  mRNA sequencing libraries were sequenced according to manufacturer recommendations with Truseq Stranded mRNA Sample kit (Illumina) at the Centre for Genomic Regulation sequencing facility in Barcelona, Spain.
GEO
GSM1576709