GSM2629347: AB1448; Mus musculus; RNA-Seq
Single-cell suspensions of tissues for all mice, excluding the Trem2 experiment, were achieved using software‐controlled sealed homogenization system (Dispomix; http://www.biocellisolation.com) in PBS, followed by density gradient separation; Pellet was mixed with 40% percoll and centrifuged. Brains of Trem2 mice were processed as previously published (Wang et al., 2015). 3' end mRNA libraries were prepared for sequencing using MARS-seq (Jaitin et al, Science 2014)
GEO
GSM2629347