GSM3357202: Ca_Cg_C_2_6h; Candida albicans; [Candida] glabrata; RNA-Seq
Biofilms were washed off of the coverslip with pre-chilled sterile MilliQ water and cells were collected by centrifugation (2000 x g, 5 minutes, 4 °C). Supernatant was removed and cell pellets were flash-frozen in liquid nitrogen prior to lyophilization for 24 hours. Acid-washed glass beads (Sigma-Aldrich) were added to the dried cell pellets and disrupted in the Disruptor Genie for 2 minute cycles for up to 10 minutes. Lysis buffer from the GE Illustra RNAspin Mini kit was added to the disrupted cell powder and RNA was extracted using the kit protocol with on-column DNase I treatment. RNA quality and concentration were determined using a Nanodrop® and Qubit™, respectively. RNA libraries were constructed using TruSeqRNA kit and processed (125SE) on an Illumina HiSeq 2500 v4 High Output instrument
GEO
GSM3357202