RNA-Seq of Neocinnamomum caudatum:developing seeds 146DAF biological replicate             2
Total RNA of the fruits was extracted and quantified. The poly-A                 tailed mRNA was purifyed from the total RNA using Dynabeads? mRNA Purification Kit.                 The fragments were used to synthesize first-strand cDNA with random primers, and                 first-strand cDNA was transformed into double-strand cDNA by using RNase H and DNA                 polymerase I. Fragments of desirable lengths (200-300 bp) were purified. Under the                 function of 3 '-5' exonuclease and polymerase, the protruding terminus of the DNA                 fragments were end-repaired and ligated with sequencing adapters through A and T                 complementary base pairing. The sequencing library was constructed using polymerase                 chain reaction (PCR). The synthesized cDNA libraries were normalized to a 10 nM and                 gradually diluted to 4-5 pM.
SUB3463476
R6b1106