GSM1214207: PaP1; Pseudomonas phage PaP1; OTHER
EDTA (20 mM), proteinase K (50 μg mL-1), and sodium dodecyl sulfate (0.5%, w/v) were added to the purified phage stock solution (PaP1 or PaP3). The mixture was incubated at 56 °C for 1 h, then an equal volume of phenol–chloroform–isoamyl alcohol solution (25:24:1) was added, followed by centrifugation at 5,000 g for 10 min. The aqueous layer was collected and extracted with chloroform at 5,000 g for 10 min. Finally, the collected aqueous layer was mixed with 0.6 volumes of isopropanol, and stored overnight at –20 °C. The next day, the mixture was centrifuged for 10 min at 12,000 g and 4 °C, and the precipitated DNA was collected and washed with 70% and 100% ethanol, respectively. The PaP1 DNA was suspended in TE buffer (pH 8.0) and stored at –20 °C for subsequent use. Genomic DNA was randomly sheared to an avearge size of approximately 2,000 bp, end repaired, and ligated to blunt end hairpin adapters for form standard SMRTbell templates
GEO
GSM1214207