GSM2628050: WT mRNA-Seq+DTT; Schizosaccharomyces pombe; RNA-Seq
Ribosome profiling lysates were clarified, RNase treated, and run over sucrose gradients to extract the monosome peak. RNA was then extracted using SDS/hot phenol/chloroform and footprints of the specified size were retained by gel purification. Briefly, RNAs were ligated to a universal DNA linker followed by reverse transcription. cDNAs were then circularized and PCR amplified. Illumina Ribo-Zero kits were used to remove rRNA (from total RNA for mRNA-Seq, from size-selected footprints for 51F-56F, and from linker-ligated footprints for 63F and 64F).
GEO
GSM2628050