GSM2803804: 23_NDC; Mus musculus; RNA-Seq
Pancreatic islets were extracted using standard pancreas perfusion with collagenase (VitaCyte). Islets were cultured in RPMI supplemented with 10% FBS, 50 U/ml penicillin, and 50 µg/ml streptomycin for approximately 48 hours, then total RNA was extracted using RNeasy Plus Mini Kit (Qiagen). RNA libraries were prepared for sequencing using standard Illumina protocols
GEO
GSM2803804