GSM5031925: M28Ag_2: Memory Day 28 + Antigen rep2 ATAC-seq; Mus musculus; ATAC-seq
Spleens from infected mice were recovered 3 hours after the peptide was administered, crushed through a nylon mesh and treated with Gey's solution red blood lysis buffer. The 2W1S-specific memory T cell pool was enriched by magnetic enrichment before purfying CD44hi 2W1S-specific CD4 T cells Cells were resuspended in 50 ul ATAC transposition reaction mix containing 25 ul 2x Tagment DNA Buffer (Illumina), 2.5 ul Tn5 transposase (Illumina), 0.5 µl 1% Digitonin (Promega) and incubated for 20-30 minute at 37C with gentle agitation. DNA was purified using the MinElute Reaction Clean up kit (Qiagen). Nextera adaptors were added during PCR amplification of transposed DNA. Amplified DNA was subsequently purified using Ampure Beads (Beckman Coulter) prior to validation.
GEO
GSM5031925