GSM5177659: Wildtype ID 526 Saline; Mus musculus; RNA-Seq
Total RNA was isolated from liver tissue. Samples were placed in 1 mL of Trizol reagent (Invitrogen, CA), then homogenized with a Tissue-Tearor homogenizer (BioSpec, Bartlesville, OK). RNA isolation was performed using manufacturer's protocol (catalog no. 15596026, Thermo Fisher Scientific). Isolated RNA samples were treated with DNase I, RNase-free (1 U/µL) (catalog no. EN0521, Thermo Scientific) following manufacturer's instruction. Samples were then processed at The Center of Functional Genomics-University at Albany. Libraries were prepared using Illumnia TruSeq Stranded mRNA using manufacturer's instruction.  Samples were sequenced using Illumina Nextseq500 Platform at 75 million single-end reads per run.
GEO
GSM5177659