GSM5077669: MECP2-WT_rep1; Mus musculus; RNA-Seq
RNA was extracted with High Pure RNA Isolation Kit (11828665001, Roche, Basel, Switzerland). Briefly, cells were scraped in PBS, lysed, and nucleic acids were immobilized by glass fiber fleece filters. Samples were treated with DNase I and RNA was washed twice before elution. RNA integrity was measured with an Agilent 2100 Bioanalyzer system. Library preparation and RNA sequencing was conducted by Novogene (Cambridge, UK). In brief, mRNA enrichment was performed with oligo(dT) bead pulldown, from where pulldown material was subjected to fragmentation, followed by reverse transcription, second strand synthesis, A-tailing, and sequencing adaptor ligation. The final amplified and size selected library comprised of 250-300 bp insert cDNA and paired-end 150bp.
GEO
GSM5077669