mRNA sequencing of AM1
Pooled PCR products were used to generate a sequencing library using the NEBNext Ultra Library Prep Kit for Illumina sequencing by applying standard protocol according to the manufacturers recommendations. Libraries were sequenced on an Illumina MiSeq platform using MiSeq Reagent Kit V3 (600 cycles) and symmetric PE-300 sequencing. All primers used in this protocol were obtained from a previously described approach
SUB7121974
AM1-206-27553-000