GSM3291007: polydom  siRNAs_rep2; Homo sapiens; RNA-Seq
Total RNA was extracted from primary cultured cells treated for 6 h with mock, 5 ng/ml β-estradiol (SIGMA-ALDRICH, St. Louis, Missouri, USA), 10 ng/ml TGF-β1 (R&D SYSTEMS, Minneapolis, Minnesota, USA), 5 μM ITD1 (TOCRIS, Avonmouth, Bristol, BS11 9QD United Kingdom), or siRNA (QIAGEN) using the Maxwell 16 LEV simply RNA Tissue kit (Promega, Madison, WI). Library preparation was performed using a TruSeq stranded mRNA sample prep kit (Illumina, San Diego, CA) according to the manufacturer's instructions.
GEO
GSM3291007