GSM2199880: FA1090cpxR_Rep2; Neisseria gonorrhoeae; RNA-Seq
Total RNA was extracted from four independent cultures of FA1090, FA1090cpxA::kan and FA1090cpxR::erm strains grown to late log phase (OD600 = 0.8-1.0) using TRIzol reagent (Invitrogen) according to the manufacturer's protocol. RNA was treated twice with the TURBO DNA-free DNase (Ambion) and the efficacy of DNase treatment was confirmed by reverse transcriptase PCR (RT-PCR) analysis and the QuantiTect SYBR green RT-PCR kit (Qiagen). Removal of rRNA from total RNA and preparation of RNA-Seq libraries were performed using the ScriptSeq Complete kit (Bacteria) (Illumina, Inc.) following the manufacturer's instructions. RNA libraries were prepared for sequencing using standard Illumina protocols.
GEO
GSM2199880