16S-rRNA of Paspalum pulchellum:Rhizosphere soil sample during the dry season
Genomic DNA was extracted and prepared using 250 mg of rhizospheric wash material. 1 uL aliquot from each sample extract was used to generate bacterial amplicon libraries. Bacterial libraries were created by PCR amplification of the V3-V4 region of the 16S rRNA gene using the universal prokaryotic 341F and 806R primers.  The PCR products were examine and mixed in at equal density ratios, then purify Region specific primers were modified to add Illumina adapter overhang nucleotide sequences to the amplicons. Following initial amplification, library size was verified on the Agilent Bioanalyzer 2100 system, and then illumina dual index and sequencing adapters were added by PCR. Quality and quantity of the libraries were assessed by using Qubit 2.0 Fluorometer and Agilent 301 Bioanalyzer 2100 system. Sequencing was done via the Illumina MiSeq sequencing platform by Metagenom Bio Inc Life Science (University of Waterloo, Canada), to generate 2  250 bp paired end reads.
SUB7342783
GGCTACGGCAAT