GSM3415041: 1157GBM; Mus musculus; RNA-Seq
Cells were lysed in Hank's Balanced Salt Solution buffer (Thermo Fisher) with 0.025% Tween (Thermo Fisher). mRNA was extracted and subjected to aRNA linear amplification by three rounds of reverse transcription, second strand synthesis and in vitro transcription, using SuperScript III First-Strand Synthesis SuperMix (Thermo Fisher), DNA Polymerase I (Thermo Fisher), T4 DNA polymerase (Thermo Fisher), 5x Second Strand buffer (Thermo Fisher) and a MegaScript kit (Thermo Fisher). DNA library was constructed with a TruSeq mRNA stranded kit (Illumina). Library was subjected to next-generation sequencing analysis in a high-throughput sequencing center with a Illumina HiSeq 4000 at the Children's Hospital of Philadelphia/Beijing Genomics Institute core facility (CHOP/BGI).
GEO
GSM3415041