GSM4399564: scRNAseq_APOE2; Mus musculus; RNA-Seq
Tumors were resected on day 19 after injection and tumor-infiltrating leukocytes were isolated with mechanic and enzymatic dissocation and gradient density centrifugation. 10,000 CD45+/DAPI- leukocytes from each mouse were independently sorted on a BD FACSAria II cell sorter and samples from the same genotype were pooled for library preparation (i.e., total of 60,000 cells per genotype). Five-thousand cells per genotype were targeted for single-cell RNA-sequencing on a Chromium Single Cell System (10x Genomics). Samples were processed as per the manufacturer's instructions (Chromium single cell 3' reagents, v3 chemistry) and libraries were sequenced on an Illumina NextSeq sequencer.
GEO
GSM4399564