GSM4718685: RNAseq - untagged ESCs day 0 - auxin - rep2; Mus musculus; RNA-Seq
Cells were rinsed once with PBS and lysed via direct addition of TRIzol reagent (Thermo Fisher Scientific). Total cellular RNA was purified using the Direct-zol RNA Miniprep kit (Zymo Research). PolyA+ RNA was isolated from 500 ng total RNA using Dynabeads Oligo(dT)25 beads (Thermo Fisher) and constructed into strand-specific libraries using the dUTP method. UTP-marked cDNA was end-repaired using end-repair mix (Enzymatics), tailed with deoxyadenine using Klenow exo- (Enzymatics), and ligated to custom dual indexed adapters with T4 DNA ligase (Enzymatics). Libraries were size-selected with SPRIselect beads (Beckman Coulter) and quantified by qPCR before and after amplification.
GEO
GSM4718685