Diesel-polluted soil microcosm experiment. Polluted non-bioaugmented control, week 9 of 12
'"Total community DNA was prepared from 0.5 g of dry soil using the PowerSoil DNA Isolation Kit (MoBio, Carlsbad, CA, USA) following manufacturer''s instructions. Mechanical disruption was performed with the FastPrep-24 bead-beater (MP Biomedicals, Santa Ana, CA, USA). V4 region of 16S rRNA gene was PCR-amplified with the primers 515F (GTGCCAGCMGCCGCGGTAA) and 806R (GGACTACHVGGGTWTCTAAT) and sequenced in the MiSeq (Illumina) platform at Argonne National Laboratory (Lemont, IL, USA) as described by Caporaso et al., 2012 (ISME J 6:1621-1624)."'
SUB1039073
C1.t9.a