GSM1571985: JT_17_RNA-seq; Drosophila melanogaster; RNA-Seq
Total fly RNA was isolated from 20 animals per sample using Trizol reagent and purified using Qiagen RNAeasy kit. Sequencing libraries were prepared from total RNA using the KAPA Stranded RNA-Seq Library Preparation Kit for Illumina platforms (KAPA Biosystems). Isolation of mRNA was done using the Dynabeads® mRNA purification kit (Invitrogen Dynal AS, Oslo, Norway), following the Appendix A in the KAPA kit protocol. All 18 libraries were indexed using standard Illumina TruSeq indexes, and sequenced altogether in one lane of a high-output paired-end 100 bp run using an Illumina HiSeq 2500. A second high-output paired-end 100 bp run of all 18 libraries was performed to increase coverage. The library preparation and the sequencing were done at the UPC Genome Core facility at the University of Southern California (Los Angeles, CA).
GEO
GSM1571985