GSM3358138: filter_Cm_ctrl; Escherichia coli str. K-12 substr. MG1655; OTHER
Cell pellets were cryogenically pulverized in a Spex 6870 freezer mill; 5 cycles of 1 min at 5 Hz with 1 min cooling. Cell lysates were clarified, digested with nucleases, and monosomes were purified over a sucrose gradient. Ribosome footprints and total RNA fragments were PAGE purified, ligated to a linker using RNA ligase T2, converted to DNA using RT, circularized, and PCR amplified. OTHER (ribosome profiling)
GEO
GSM3358138