MiSeq run for N.ditissima isolate R09/05
The isolate R09/05 was isolated in 2005 from canker infected wood of Malus domestica cv. ‘Cox’ in Kent, United Kingdom. A single ascospore culture of R09/05  was used for the genome sequencing. Genomic DNA was extracted from freeze dried mycelium using a modified protocol based upon a modified protocol from the GenElute Plant DNA Miniprep Kit (Sigma). The manufacturers protocol was used with the following modifications: The volume of lysis solutions (PartA and PartB) were doubled; an RNase digestion step was performed as suggested in the manufacturers protocol; twice the volume of precipitation solution was added; elution was performed using elution buffer EB (Qiagen). Genomic libraries were prepared using a Nextera Sample Preparation Kit (Illumina), following the manufacturers protocol.
East Malling Research
R09/05 MiSeq Run