WGS of ATCC25872-BlokeschLab
gDNA extracted from cultures grown overnight in LB medium. 2ml of cells culture were used after centrifugation. Genomic DNA kit (Qiagen) using genomic-tip of 100/G used for extraction. 4 micrograms of the DNA was used to prepare a SMRTbell library with the PacBio SMRTbell Template Prep Kit 1 (Pacific Biosciences, Menlo Park, CA, USA) according to the manufacturer's recommendations. The resulting library was size selected on a BluePippin system (Sage Science, Inc. Beverly, MA, USA) for molecules larger than 18 kb. It was sequenced with v2.1/v2.1 chemistry and diffusion loading on a PacBio Sequel instrument (Pacific Biosciences, Menlo Park, CA, USA) at 600 min movie length, using one SMRT cell v2.
SUB6805009
ATCC25872 (276; m54161_180613_130803)