GSM3454492: Ouabain16h_Rep2; Homo sapiens; RNA-Seq
Cells were scraped and homogenised in TRIzol® RNA isolation reagent, mixed with Chloroform in a 5:1 ratio, centrifuged at 15,000 rpm for 30 min and after successful phase separation, the top clear RNA-containing phase was purified using the RNAeasy® Mini Kit (Qiagen) Libraries were prepared according to Illumina's kit instructions (mRNA stranded library prep kit)
GEO
GSM3454492