RNAseq of Senecio spp (young plants)
Total RNA extacted and treated with  DNase, mRNA was selected and converted to cDNA. Second strand cDNA synthesis  to incorporate dUTP. The cDNA was end-repaired, A-tailed and adapter-ligated.  Prior to amplification, samples underwent uridine digestion. The prepared  libraries were size selected, multiplexed and QCed before paired end  sequencing.
SUB5841939
BN658