GSM3291101: Untreated at 72hrs Replicate 2; Homo sapiens; RNA-Seq
RNA extraction was performed with QIAzol (custom method using Qiagen cat# 79306). The yield and quality of the isolated RNA was assessed using Qubit Fluorometer Qubit 2.0 fluorometer (Thermo Fisher Scientific?Waltham, MA, USA) and Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), respectively. 1 ug of RNA was used for cDNA library construction using an NEBNext® Ultra Directional RNA Library Prep Kit for Illumina® (cat# E7420S, New England Biolabs, Ipswich, MA, USA) according to the manufacturer's protocol. The resulting 250-350 bp insert libraries were quantified using a Qubit 2.0 fluorometer (Thermo Fisher Scientific?Waltham, MA, USA) and quantitative PCR. Size distribution was analyzed using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Qualified libraries were sequenced on an Illumina HiSeq 4000 Platform (Illumina, San Diego, CA , USA) using a paired-end 150 run (2×150 bases). 40 M clean reads were generated from each library.
GEO
GSM3291101