exosomal miRNA of PC-1.0 pancreatic cancer cell
Samples of PC-1.0 exosomes and PC-1 exosomes were prepared for high-throughput sequencing. In brief, total RNA of each sample was extracted using TRIzol  Reagent (invitrogen) and then 2 libraries were constructed using smRNA-seq(for illumina) (clontech, CA, USA) according to the manufacturer's instruction. RNA-seq was performed using illumina HiSeqTM 2500(Illumina, Inc., San Diego, CA, USA). Raw data was processed to identified differentially expressed miRNAs(DEMs). DEM data was assigned for further bioinformatic analysis.
SUB3599869
PC-1.0 exosomal miRNA