GSM5456189: WTS42b-DIV-150-SampleB (2 slices); Homo sapiens; RNA-Seq
Dissociation of the slices was achieved by Accumax (Sigma, A7089) using the gentleMACS Octo Dissociator. The cell suspension was run through a 70μm strainer prior to processing through the 10X Genomics platform. scRNA-seq libraries were prepared for sequencing using the 10X Genomics Chromium Single Cell 3' Library & Gel Bead Kit (10X Genomics, 120237) workflow. The cell suspension was loaded onto a 10X Genomics Chromium Single Cell 3' Chip with the appropriate amount of Mastermix. RNA extraction was performed following the same 10X Genomics Chromium workflow (Next GEM sequencing v3.0 for batch 1 and 3.1 for batch 2). The reverse transcriptase reaction and subsequent amplification was carried out in a C1000 Touch Thermal Cycler (Biorad), with the cDNA being amplified for 12 cycles. Before sequencing, libraries were quality tested using the 2100 Bioanalyzer Instrument (Agilent) and their concentration was measured by qPCR.
GEO
GSM5456189