GSM5077476: Yohei-02_LNCaP_no_dox; Homo sapiens; RNA-Seq
RNA was isolated by the RNeasy Mini Kit (Qiagen). DNA was removed by treating with DNase from the kit (Qiagen). cDNA was generated using All-In-One RT MasterMix (ABM, G490). qRT-PCR was performed with 1x SYBRTM Green PCR Master Mix (Thermo, 4309155) using primers that had been optimized for their melt curve. Libraries were generated using Illumina's TruSeq kit
GEO
GSM5077476