GSM5262026: SMS.Etoposide_3h; Homo sapiens; ncRNA-Seq
Total RNA was extracted using TRNzol Universal (Tiangen, DP424) and Total RNA kit I (Omega, R6834-02) according to the manufacturer's protocols and quantified using Qubit 3.0 Fluorometer (Life technologies). Total RNA (5 μg) was treated with 5 U of TURBO DNase (Thermo Fisher Scientific, AM2238) in 1× TURBO DNase buffer (Thermo Fisher Scientific, AM2238) at 37°C for 30 min to remove genomic DNA, and then purified with 2× volumes of VAHTS RNA Clean Beads (Vazyme, N412). Total RNA samples were used for cDNA synthesis performed with a modified version of the not-so-random hexamer method to avoid synthesis of rRNA. The cDNA samples were polyA−tailed with TdT, blocked and used directly for sequencing on the Helicos/SeqLL SMS platform.
GEO
GSM5262026