GSM5222172: Mature_spherule_CDS_2; Coccidioides immitis RS; RNA-Seq
Mycelia and spherules were stored in QIAzol (Qiagen) at -70°C. Samples were added to 2 ml ZR BashingBead lysis tube with 0.5 mm beads (Zymoresearch) and tubes arranged in a pre-cooled Tissuelyzer II adapter (Qiagen) and disrupted by shaking at 50 Hz for 25 min. Total RNA was purified from mycelia and spherule samples (2 replicates / condition) using chloroform extraction and isopropanol precipitation and quantified using a Qubit 3.0 Fluorometer (Invitrogen) Strand-specific, paired-end libraries were prepared from total RNA by ribosomal depletion using the Yeast Ribo-Zero rRNA Removal Kit (Illumina) and using the TruSeq Stranded total RNA-Seq kit (Illumina).
GEO
GSM5222172