GSM1573677: S12_Normal_2; Shewanella decolorationis S12; RNA-Seq
Total RNA from the cell pellet was isolated using a TRIzol Reagent kit (Invitrogen, Carlsbad, USA) following the manufacturer’s instructions. RNA samples were treated with RNase-free DNaseI (QIAGEN, Carlsbad, USA) to digest residual genomic DNA and then purified by an RNeasy kit (Qiagen, Valencia, USA). Ribosomal RNA was removed by a RiboZero™ rRNA Removal kit (Epicenter, Madison, USA) following the manufacturer’s protocols (Bhagwat et al., 2014). RNA libraries were prepared for sequencing using standard Illumina protocols
GEO
GSM1573677