Microbiome of a watershed
Samples were extracted using the Qiagen PowerSoil MagAttract KF kit. A single step PCR reaction, using the primers 515f and 806r and a single 12-bp barcode scheme, was performed to amplify the v4 region of the bacterial 16S gene. PCR products were cleaned using AMPure XP beads, normalized with Charm Biotech Just-a-Plate, and paired end sequenced for using the hiseq2500 platform.
SUB9061153
104052