GSM5081666: h2a.w-2 ATAC-seq rep 1; Arabidopsis thaliana; ATAC-seq
Nuclei were extracted from 0.5 g fresh 10-day-old seedlings, DAPI stained and sorted on FACS Aria III (BD Biosciences) ATAC-seq was performed as described in Lu et al. 2017 (PMID: 27903897). Tagmentation reaction was carried out on 50,000 sorted nuclei using Tn5 transposase (Nextera cat. no. 15027865), and tagmented DNA was isolated using the MinElute PCR purification kit (Qiagen). Libraries were amplified using NEBNext 2x master mix (NEB) and Nextera primers, and sequenced on NextSeq 550 (Illumina).
GEO
GSM5081666