RNA-Seq of cowpea leaves under mechanical injury and virus inoculation (CPSMV)
Total RNA was isolated using the SV Total RNA Isolation System kit (Promega, US) following manufacturer's protocol. Agarose gel (1.5 %) and Agilent 2100 Bioanalyzer (Agilent Technologies, EUA) evaluated both concentration and quality of total extracted RNA. Only samples with RNA integrity number (RIN)  8.0 were sequenced. A RNAm TruSeq Stranded LT-Set A kit (RS-122-2101) (Illumina, San Diego, CA, EUA) was employed in messenger RNA purification and cDNA library construction according to the manufacturer's instructions. Paired-end reads, with 100 pb in length were generated via the Illumina HiSeq 2500 system, using the following kits: HiSeq Rapid PE Cluster Kit v2 (PE-402-4002); SBS Kit v2 (200 Cycle; FC-402-4021); and TruSeq Stranded mRNA LT - Set A (RS-122-2101). All sequencing steps were performed at the Center for Functional Genomics, University of So Paulo (So Paulo, Brazil).
SUB7902786
Cowpea_Leaves_AbsCont_60m_RB2_1