GSM1573616: peroneal longu; Sus scrofa; miRNA-Seq
Total RNA was extracted from PL using the mirVana™ miRNA isolation kit (Ambion, Austin, TX, USA) following the manufacturer’s procedure. The quantity and purity of total RNA were monitored by NanoDrop ND-1000 spectrophotometer (Nano Drop, DE, USA) at 260/280 nm (ratio > 2.0). The integrity of total RNA was monitored by the Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number > 6.0. Equal quantities (5 µg) of small RNA isolated from three female Landrace pigs were pooled. Briefly, approximately 15 µg of small RNA was used for library construction and sequencing. The small RNA fragments (between 10-40 nt) were isolated by polyacrylamide gel electrophoresis (PAGE) and ligated with proprietary adaptors (Illumina, San Diego, CA, USA). The small RNA fractions were then converted to cDNA by RT-PCR and the cDNA was sequenced on the Genome Analyzer GA-II (Illumina) following the recommended manufacturer’s protocol.
GEO
GSM1573616