GSM3666325: HAEC_1; Homo sapiens; RNA-Seq
RNA was isolated from cultured endothelial cells using the miRNeasy micro Kit (Qiagen) combined with on-column Dnase digestion (DNase-Free DNase Set, Qiagen) 4µg of total RNA was used as input for Truseq Stranded mRNA Library preparation following the low sample protocol (Illumina). Sequencing was performed on the NextSeq500 instrument (Illumina) using v2 chemistry, resulting in minimum of 37M reads per library with 1x75bp single end setup.
GEO
GSM3666325