GSM3898377: cbp-ifKO_ChIP-aP300_1; Mus musculus; ChIP-Seq
Hippocampi were micro-dissected after cervical dislocation, washed in ice-cold PBS and pooled together, when fixation was needed 1% FA was used. Nuclei were isolated using our improved FANS protocol for SUN1-GFP+ cells. Extensive description of protocols indicated in Supplemental Experimental Procedures. ChIP-seq was conducted as previously described (Lopez-Atalaya et al., 2013) using the anti-H3K27AC (AB4729 Abcam), anti-H3K9,14 (Home made), anti-CBP (sc-583) and anti-p300 (sc-585). Libraries were prepared with TruSeq.
GEO
GSM3898377