Genome sequencing of the holosporalean 'Candidatus Finniella inopinata'
For long-read sequencing, DNA from axenic cultures of Viridiraptor invadens strain Virl02 (the host of Candidatus Finniella inopinata) was extracted using a CTAB and phenol-chloroform method. Total gDNA was further cleaned through a QIAGEN Genomic-Tip 20/G. A sequencing library was made using the Nanopore Ligation Sequencing Kit 1D (SQK-LSK108). Culturing was done by Sebastian Hess (Centre for Comparative Genomics and Evolutionary Bioinformatics, Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Canada). DNA isolation, sequencing, and assembly was performed by Sergio A. Munoz-Gomez (Centre for Comparative Genomics and Evolutionary Bioinformatics, Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Canada).
Center for Comparative Genomics and Evolutionary B
HOLO02