diapause migratory locust eggs stage 1 transcriptome
More than 2000 locust eggs were collected from the same generation, divided into two parts and reared at the two growth cabinets for a whole generation. After adults ovipositing, we recorded the hatchlings on each day of the two growth cabinets respectively. We found diapausing rate of overwintering eggs at low temperature regime was 64.3% (diapause eggs, only 35.7% hatchlings emergence in the growth cabinet), while all eggs hatched at high temperature regime (non-diapause eggs, 100% hatchling emergence in the growth cabinet). At the same time, diapause eggs were collected respectively when DD reached to 50 heat units, and snap-frozen immediately in liquid nitrogen and stored at -80℃.
Chinese Academy of Agricultural Sciences
diapause