Illumina HiSeq 2500 paired end sequencing; Single-cell RNA-seq of dermal fibroblasts (5 human individuals - IFNB stimulation)
Single-cell RNA-seq of dermal fibroblasts (5 human individuals - IFNB stimulation)
Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin – Streptomycin (Life Technologies / 15140122).  Cells were stimulated with 1000 IU IFNB (human IFNB - R&D Systems / 11410-2) for 2 or 6 hours Reverse transcription and cDNA amplification was performed according to the SmartSeq2 protocol (Picelli et al., 2014) Library preparation was performed using an Illumina Nextera kit.
EMBL-EBI and Wellcome Sanger Institute
E-MTAB-7051:22728_6_245_p