GSM5076884: GFP_1M7_Shape-map; Homo sapiens; HIV-1 vector pNL4-3; OTHER
RNA were recovered from infected cells and splitted in 3 samples: one was trated with 1M7, the second with DMSO and the third with 1M7 in denaturing conditions according to the SHAPEMap protocol as described by smola et al (2015) Nature protocol 10, 1643. After treatment RNA were reverse transcribed with the primers described in Watts et al. (2009) Nature, 460, 711 under the conditions described by Smola et al. (2015); The NGS librairy were then obtained using the appropriate kits from New england Biolabs following the manufacturer instructions RNA Library Prep kit for Illumina (New England Biolabs)
GEO
GSM5076884