miRNA-seq of Jatropha curcas: young seeds
The sRNA libraries were generated using NEBNext? UltraTMsmall RNA Sample Library Prep Kit for Illumina? (NEB, USA) following the manufacturer¡¯s recommendations. In brief, RNA bands corresponding to a size range of 16¨C30 nt were separated and purified from the acrylamide gel. The small RNA molecules ligated with 5¡¯and 3¡¯ adaptors were used for reverse transcription and subsequent PCR. Small RNA samples were sequenced using Illumina HiSeq2500 platform (San Diego, CA, USA) and single-end reads were generated.
SUB3101956
Young