ddRADseq of Papilio glaucus and Papilio canadensis musuem specimens
Two reduced-complexity libraries were generated for 101 individuals using a variation of the Restriction-Site Associated DNA fragment (RAD) procedure (adapted from Nosil et al. 2012) involving a double digest (ddRADseq; Parchman et al. 2012). Briefly, approximately 600 ng of genomic DNA was digested using the restriction enzymes EcoRI and MseI. For each individual, the EcoRI adapter contained a unique 8 to 10 base pairs (bp) index for barcoding. PCR product was then pooled (into a five and 96 individual library), purified with QIAquick Purification Kit (Qiagen), and size selected (400-600 bp range) on a BluePippin System (Sage Science).
SUB3600964
PAP214OLD