GSM2803244: PBS 3hours, biological rep1; Mus musculus; RNA-Seq
Total RNA was extracted following the manufacturer´s kit, RNeasy Mini Kit from Qiagen. An additional step of genomic DNA elimination was included before the RNA elution using the RNase-Free DNase set (Qiagen). Eluted RNA samples were assayed with an absorbance at 260/280 nm between 1.8 and 2.0 and were subjected to a strict quality control at the Australian Genomic Research Facility (AGRF) in Perth before proceeding to library preparation and RNA Sequencing at the AGRF in Melbourne. All submitted samples had RNA integrity number (RIN) of 10. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO
GSM2803244