RRBS analysis of WT ES cells
DNA extracted from WT ES cells were first digested with MspI restriction enzyme, followed by the ligation with methylated adaptors at the both end of DNA fragments. DNA fragments of 150-250bp in length were then subjected to bisulfite conversion and amplified by PCR. Sequence was performed at 36bp single end protocol under illumina platform.
HIROSHIMA
DRX016199