"Post-treatment tumor WXS in a patient with small cell lung cancer, liver lesion"
"Formalin-fixed, paraffin-embedded (FFPE) tumor tissue samples were prepared for whole exome sequencing (WES). One hundred nanograms of DNA was sheared to approximately 200 base pairs (bp) by sonication (Covaris, Woburn, MA). Exome enrichment was performed using SureSelect Clinical Research Exome Kits according to the manufacturer's instructions (Aglient, Santa Clara, CA). Paired-end sequencing (2 x 75 bp) was performed on an Illumina NextSeq500 instrument. The sequences were compared to the human reference genome hg19 using internally developed ClinOmics somatic Bioinformatic Pipeline v3.1. In brief, raw sequencing data in FASTQ format were aligned against the reference human genome (hg19) with BWA (Li and Durbin 2009)."
NIH-phs002089
S2