total RNA: EBOV-Minigenome with NheI-Mutation transfected HEK293 cells, no VP30
Poly(A)+ RNA was isolated. First-strand cDNA synthesis was primed using a N6 randomized primer. After fragmentation, the Illumina TruSeq sequencing adapters were ligated in a strand specific manner to the 5' and 3' ends of the cDNA fragments. The cDNA was finally amplified with PCR using a proof reading enzyme. For Illumina sequencing, the cDNA samples were size fractionated using a differential clean-up with the Agencourt AMPure kit. The size ranges chosen were 350 - 700 bp for the random- primed cDNAs.
SUB9183141
NheI_minusVP30_total_T1647_S8