GSM4989498: TetTWT-Th2-1st-BS-rep1; Mus musculus; Bisulfite-Seq
Genomic DNA (gDNA) was isolated by PureLink Genomic DNA Mini Kit (Thermo Fisher Scientific). 0.5 μg of gDNA samples were treated with sodium BS (MethylCode Bisulfite Conversion kit; Invitrogen). The PCR amplicons were generated using the PyroMark PCR kit (QIAGEN) and quantified using Qubit assays (Invitrogen). PCR amplicons were pooled and then used for library preparation using NEB Next DNA Library Modules for Illumina platform (New England Biolabs, Inc.). bisulfite or pyridine borane sequencing
GEO
GSM4989498