GSM3453132: Bulk_pre-DC_rep2 [307_AAGAGG]; Mus musculus; RNA-Seq
Total RNA obtained from FACS-sorted cells was purified with the Absolutely RNA Nanoprep kit (Agilent), quantified with the NanoDrop spectrophotometer (Thermo Scientific) and measured for quality control by the Agilent Bioanalyzer. Samples with RIN (RNA integrity number) scores 8.8 and higher were further processed. 6ng starting material was reverse transcribed and amplified to 5-7µg cDNA with the RNA Ovation RNA-seq System V2 (NuGEN). From 100ng amplified cDNA sequencing libraries were generated with 16 unique barcoded adapters (8 samples x 2 replicates) using the Ovation Ultralow DR Library System (NuGEN).
GEO
GSM3453132