In vivo RNA structure probing with DMS and high-throughput sequencing
High-throughput sequencing libraries were prepared using a custom protocol. Cells were treated with DMS (100 mM) or untreated (No DMS) and RNA was purified using homogenization with glass beads. Total RNA was randomly fragmented and RNA adapter oligonucleotides were ligated to RNA fragments. Sequencing libraries compatible with Illumina Truseq were prepared essentially as described by Ingolia and Colleagues (Cell 2011).
Carnegie Mellon University
Scer_L26_Delta_Rep1_100mM_DMS