ITS library of coastal seawater - 07 Nov 2012 (weekly sample)
We extracted DNA using phenol-chloroform chemical extraction (Massana et al., 1997) supplemented with three rounds of bead beating (30 seconds each). The fungal internal transcribed spacer (ITS) region was amplified using the dual index PCR primers ITS1-F 5 -CTTGGTCATTTAGAGGAAGTAA-3  and ITS4 5 -TCCTCCGCTTATTGATATGC-3  (Bruns & Gardes, 1993)
SUB3610012
fungi_ITS1_4_PICO484