coral larvae hypoxia
Coral larvae were drained of RNALater. Total RNA was extracted using the Qiagen RNeasy mini kit. RNA concentrations were assessed using a NanoDrop ND-1000, and RNA quality was evaluated through gel electrophoresis via the presence of intact 18S and 28S ribosomal RNA bands using an Agilent 2100 BioAnalyzer (Agilent Technologies). An Illumina TruSeq RNA prep kit was used to separate the mRNA from the total RNA via polyA selection and to generate 2x150bp long paired-end libraries for each sample with an average library size of 364 bp. Sequencing was performed on the Illumina HiSeq 2000 sequencer at the BioScience Core Lab (BCL) at the King Abdullah University of Science and Technology (KAUST).
SUB9521372
ASL-T2-T4