Telomere sequencing of Schizosaccharomyces pombe: 18000STED
Genomic DNA (gDNA) libraries were made using the Illumina TruSeq Nano DNA Library Prep kits as follows: S.pombe genomic DNA was fragmented to a 400 bp insert size, blunt-ended, and size selected using sample purification beads. Indexed adapters were ligated to the ends of the DNA fragments and PCR-amplified to enrich for fragments having adapters on both ends. The final purified product was then quantitated by qPCR before cluster generation and subsequent paired-end sequencing.
SUB9312443
18000STED