Borrelia burgdorferi sensu stricto Europe genome sequencing
DNA samples were prepared according to Nextera DNA sample preparation guide (Qiagen). The samples were diluted to 50ng/μl concentration and “tagmented” by simultaneously fragmenting DNA using transposomes and adding adapters. After tagmentation samples having adapters on both ends underwent 5 PCR cycles to amplify the product. The sequences were indexed and the resulting libraries were validated using Agilent 2100 Bioanalyser.
Bavarian Health and Food Safety Authority
Bbss_PFhe_I