GSM3723814: MDA231: IVMS2; Homo sapiens; miRNA-Seq
MDA231 Cells were lysed in an ice-cold lysis buffer (20 mM Tris•Cl pH7.5, 50 mM NaCl, 5 mM MgCl2, 0.5% NP-40) containing protease inhibitors and RNasin (200 U/mL). Lysates were centrifuged at 12,000 rpm at 4°C for 15 minutes to remove cell debris. Cell lysates were incubated with RNA conjugated Amylose resin at 4°C for 5 h with gentle rotation. After washed with wash buffer, the Amylose resin complexes  were eluted with 100 μl lysis buffer containing 10 mmol/L maltose. The eluted samples were further subject to RNA purification for miRNA-seq. Libaries were prepared for sequencing according to Illumina's protocol. The experiment was performed by the Guangzhou RiboBio co. Ltd in Guangzhou, China.
GEO
GSM3723814