GSM2629893: RIL165_2; Zea mays; RNA-Seq
A single leaf extract from three plants per biological replicate (field block) were combined.  The leaf extract consisted of a 12cm2 section of a single leaf displaying grey leafspot symptoms.  RNA was extracted from pooled leaves in each biological rep using  Qiazol and total RNA was purified using the RNeasy Mini kit (Qiagen). Total RNA (1ug per sample) was used to prepare 50 bp short-insert libraries using the Illumina TruSeq RNA Sample Prep Kit and sequencing was performed on an Illumina HiSeq™ 2000 system at BGI.
GEO
GSM2629893