E13.5 male GCs, H3K27me3 ChIP #2
Male C57Bl/6J mice homozygous for an Oct4-EGFP transgene were mated to wild-type C57BL/6 females, and gonads were taken from resulting embryos at the indicated time point.  GFP-positive  germ cells were isolated by flow cytometry.  ChIP was performed on 50,000 cells after formaldehyde cross-linking.  Libraries were produced using the Illumina TrueSeq DNA sample kit (FC-121-2001) according to the manufacturer’s instructions.  No amplification beyond that used in standard protocols was used for library preparation.
Whitehead Institute for Biomedical Research
E13.5_male_K27_2