RNA-seq of Odontomyia cf. opertanea
Specimens were extracted with TRIzol reagents (Invitrogen,  Grand Island, NY, USA)at the Beijing Genomic  Institute (BGI, China). mRNA was selected with Dynabeads mRNA Purification  Kit (Invitrogen, Grand Island, NY, USA) and sheared with RNA  fragmentationreagents (Invitrogen, Grand Island, NY, USA). cDNA  libraries were built with Illumina TruSeq kits and equimolarly pooled with  eleven distantly related species for 150bp paired end sequencing. These pools  included other flies, beetles and moths that are not part of this  study.
SUB8614524
CWHPE17040181