MinION sequencing of PAO bioreactor fed wih alternative carbon sources
Total genomic DNA was extracted from sampled reactor biomass with modified xanthogenate-SDS protocol. The sequencing library was constructed from 1.5 ug of HMW DNA using SQK-LSK108 ligation sequencing kit. Approximately 400 ng of library was loaded on FLO-MIN106 flowcell FAH01680.