GSM3294882: LM3; Bos taurus; miRNA-Seq
Total RNA was isolated from ~100 mg tissue using the mirVana miRNA Isolation Kit (Ambion® , Life Technologies, Carlsbad, CA, USA) following manufacturer's protocol. The RNA concentration was determined using a Qubit 2.0 Fluorometer (Life Technologies). The quality of RNA was evaluated using the Agilent 2100 Bioanalyzer (Agilent Technologies, CA, USA) with the RNA 6000 NanoLabchip Kit. RNA samples with the RNA integrity number (RIN) more than 7.0 were used for small RNA library construction and quantitative real-time PCR validation. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM3294882