GSM2632185: LWH_rep1; Sus scrofa; RNA-Seq
Total RNA was extracted using TRIZOL Reagent (Cat#15596-018, Life Technologies, Carlsbad, CA, US) following the manufacturer’s instructions and checked for a RIN number to inspect RNA integrity by an Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, US). Qualified total RNA was further purified by RNAClean XP Kit (Cat#A63987, Beckman Coulter, Inc. CA, US) and RNase-Free DNase Set (Cat#79254, QIAGEN, GmBH, Germany). RNA-seq libraries were prepared using TruSeq RNA Sample Pre Kit v2 –Set A/ B (Illumina).
GEO Accession
GSM2632185