RNA-Seq of B16 melanoma tumor: VEGFB167 Heterozygous mus musculus
Sequencing libraries were generated using NEB Next Ultra TM RNA Library Prep Kit from Illumina (NEB, USA) following the manufacturer's recommendations. Briefly, mRNA was purified from total RNA; mRNA was enriched via magnetic oligo beads and then fragmented and broken into short fragments. Double-stranded cDNA was synthesized by using random hexamer primer. After that, the NEBNext sequence adapter containing index sequences hybridized to the ends of cDNA, and the desired fragments were separated using AMPure XP beads ((Beckman Coulter, Brea, CA, USA). Finally, the cDNA libraries were sequenced using the BGISEQ-500 platform and 100 bp single-end reads were generated.