RNA-Seq of Bothrops neuwiedi: adult female gland
We sprayed the venom glands manually in the presence of liquid nitrogen and total RNA was isolated according to the Trizol reagent method. We perform selective depletion of ribosomal RNA and enrichment of mRNA. We purified the cDNA and prepared it for chip 318 deposition to large-scale sequencing. After we considered only sequences with phred quality greater than 20.