MNase-Seq of saccharomyces cerevisiae: arp8D H2A nucleosomes: Sample arp8DELTA AA nucleosomes
"arp8D strain was grown to log phase and fixed with paraformaldehyde. Mononucleosomes were released by MNase digestion and FLAG epitope-tagged H2A.Z-containing nucleosomes were quantitatively immunoprecipitated using anti-FLAG agarose and eluted using FLAG peptides. The flow-through, enriched for H2A nucleosome-associated DNA fragments, was mapped by deep sequencing."