GSM5732002: RMRP_SHAPE_WT_TREATED_2; Homo sapiens; OTHER
Total RNA was extracted using Trizol, following manufacturer's phase separation protocol. RNA was reversed transcribed using an RMRP specific RT primer (5'-ACAGCCGCGCTGAGA-3'). Two sequential PCRs were then performed to ligate adaptors for sequencing.
GEO Accession
GSM5732002