GSM3294429: RNA-seq of dbr1-2 with Rnase R digestion replication 2; Arabidopsis thaliana; RNA-Seq
Total RNA were extracted with Trizol (Amion) Total RNA isolated from Col-0 and dbr1-2 was treated with a Ribo Zero kit (Epicenter) to obtain a ribosomal RNA-depleted RNA (ribo- RNA). Then ribo- RNA was incubated with RNase R (Epicentre) and then subjected to phenol:chloroform purification. Purified RNAs were further used for library preparation with Illumina TruSeq Stranded Total RNA HT Sample Prep Kit (P/N15031048), and then subjected to deep sequencing with Illumina HiSeq 2000 at Genergy (Shanghai, China).
GEO Accession
GSM3294429