TG1203D3293
The amount of DNA was quantified using athe Qubit fluorometer dsDNA assay Kit (Invitrogen, CA), as well as Infinite F200 Pro NanoQuant (TECAN, Männedorf). High molecular weight genomic DNA was sheared using a Covaris S2 Ultrasonicator and used in the preparation of the whole genome shotgun libraries according to Illumina’s library preparation protocols (Illumina, CA). The efficacy of each step of library construction was ascertained by Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara).