Bacterial diversity and gene pool in a 20 m deep well ata Merida, Yucatan
The sample was taken from a well 30 m total depth. The water was pumped from a depth of 20 m and filtered sequentially through filters of 20, 5 and 0.2 um. Metagenomic DNA was extracted from the 0.2 filter, using the DNA Isolation Kit ® PowerSoil The sequencing library was constructed according to the protocol of the GS Titanium General Library Prep Kit (Roche Applied Science). After titration of the library using the GS Titanium emPCR SV Kit, a full sequencing run was carried out on the GS FLX Titanium platform.