Single-cell RNA-Seq of Zea mays SAM and plastochrons 1-2
Cells were generated through protoplasting of the maize seedling SAM plus plastochrons 1-2. Viable protoplasts were manually washed and harvested into 96-well plates and mRNAs captured and synthesized into sequencing libraries using Cel-Seq2. Read 1 (R1) contains the Illumina index and Read 2 (R2) contains the cell barcode, UMI, and transcript sequences.