RNA-seq of TPC1-KO MEF cells, replicate 2
For RNA sequencing amplified cDNA was prepared from 5 ng of total RNA according to the manufacturers protocol (Ovation SoLo RNA-Seq, NuGen). 11-14 PCR cycles were used for library enrichment. Agencourt AMPure XP Beads (Beckman Coulter) were used for purification.