GSM1362981: 2.24 kg/ha glyphosate Rep-3; Euphorbia esula; RNA-Seq
Total RNA was purified from aerial tissue samples according to the pine tree RNA extraction protocol (Chang et al. 1993). The RNA was treated with amplification grade DNase1 (Invitrogen), quantified by the Qubit® 2.0 Fluorometer, and quality was confirmed by agarose gel electrophoresis. The RNAseq libraries were prepared with the TruSeq Stranded mRNA LT Sample Preparation Kit (Illumina, Cat. N° RS–122–2101) starting from 1ug of total RNA. The pooled cDNA library was quantitated by qPCR using the Library Quantification kit (Kapa Biosystems, Cat. N° KK4824). The size range of the final cDNA libraries was determined on an Agilent bioanalyzer DNA7500 DNA chip (Agilent Technologies). The cDNA libraries were sequenced on one lane for 151 cycles from each end of the cDNA fragments on a HiSeq2500 using a TruSeq SBS sequencing kit version 1 (Illumina).
GEO Accession
GSM1362981