RNA-seq of mus musculus: B16F10-PoleP286R tumors
Mouse tumors were trimmed, dissected, and then chopped to small pieces with blade in Petri dishes. Tumor pieces were further mixed and snap frozen with dry ice to reduce tumor regional heterogeneity. The frozen tissue was used for isolation of DNA and RNA and submitted to the Integrated Genomic Core for library preparation and sequencing. Three replicates per condition were submitted for sequencing.