RNA-Seq of Human RPTE cells, cytoplasmic fraction (BKV infection)
Extraction of cytoplasm from whole cells was achieved with NE-PER Pierce kit and the corresponding RNA was extracted with TRIzol (Ambion), and then purified with Direct-zol columns (Zymo Research) according to the manufacturers instructions. Resulting samples were submitted to the Genomics Research Core facility at the University of Pittsburgh for construction of paired-end (2x 75bp) RNA-seq strand specific libraries using enriched polyadenylated RNA and Illumina Sequencing.