Viral metagenome from organic waste: baseline Nanopore
DNA extraction was done by phenol:chloroform method. The aqueous phase was transferred to a new tube and the DNA was precipitated standard EtOH precipitation. The DNA pellet was dissolved in sterile MilliQ water. A barcoded Nanopore library was build for the baseline virome using Rapid Barcoding Sequencing kit (SQK-RBK004) and sequenced on the MinION platform using an R9.4 flow cell (Oxford Nanopore Technologies, Oxford, UK).