RNA-seq of human: child leukocytes: Sample AL4-D701-D504
Total RNA was extracted using LeukoLOCK (Thermo Fisher), which captures total RNA from neutrophils, eosinophils, basophils, monocytes, and lymphocytes, and preserved at −80°C. All RNA samples had a RNA Integrity Number (RIN) of at least 6 measured on Agilent Bioanalyzer. Library preparation was performed in batches of up to 96 samples (with multiple samples from the same participant always processed within the same batch) on 1–4 μg total RNA, per standard Illumina TruSeq Stranded mRNA library preparation protocol, and paired-end sequenced on Illumina NextSeq500 using NextSeq 500 High Output v2 Kit (150 cycles) to average depth of 40M reads.
alignment_software
HTSeq