GSM4404907: shLmna rep1; Mus musculus; RNA-Seq
RNA was isolated using Dynabeads mRNA Direct Kit (Thermo Fisher Scientific) following manufacturer's instructions. We used a derivation of the MARS-seq as described (Jaitin et al. Science 2014), developed for single cell MARS-seq to produce 3' expression libraries. 3' RNA-seq for digital gene expression quantitation (single end protocol R2 used for 7N barcode + 8N UMI in fastq header)
GEO Accession
GSM4404907