GSM1368208: gamma8-input; Mus musculus; ChIP-Seq
Cellular lysate was prepared by sonication in detergent-containing buffer after fixation with 0.5% formaldehyde at room temperature for 5min, and then DNA/protein complex was obtained by enzymatic digestion. To repair ChIP-DNA and input-DNA ends, T4 DNA polymerase, Klenow enzyme and T4 polynucleotide kinase were used (New England Biolabs), and an A base was subsequently added to the 3'-end by the treatment of Klenow exo-. The adaptor ligation and the PCR amplification for 18 cycles of DNA fragments were performed with NEB Next Multiplex Oligos for Illumina (Index Primers Set1).
GEO Accession
GSM1368208