DPH-2
The 16S rDNA V3-V4 region of the bacteria was conducted by PCR with primers 341F and 806R. Purified amplicons were paired-end sequenced (2  250) on an Illumina Hiseq2500 platform. After reads filtering, Flash software v1.2.11was used to merge paired and clean reads with a minimum overlap of 10 bp and mismatch error rates of 2% and QIIME v1.9.1 was used to filter noisy sequences. UPARSE pipeline was used to cluster the effective tags into operational taxonomic units (OTUs) of  97% similarity (Edgar, 2013).