GSM5345351: LMN09-T; Mus musculus; RNA-Seq
Total RNA for Library Preparation and Sequencing analysis was purified from microdissected VcMsh2 FFPE tumor and normal intestinal tissue using the RNAeasy Plus Kit (QIAGEN) that included a genomic DNA elimination step. Coding RNA transcripts were enriched using mouse exome panel hybridization (Twist Biosciences).  Size, concentration, and integrity was verified using Agilent 2100 Bioanalyzer (Agilent Technologies). Libraries were generated using Illumina's TruSeq RNA sample Prep Kit v3, following the manufacurer's protocol. Sequencing of 8–10 pM of each library was done on the NovaSeq sequencer as 75bp paired-end read runs.
GEO Accession
GSM5345351