GSM5345279: D21_SPL_1; Mus musculus; RNA-Seq
For isolation of CD4+ T cells from spleen, spleens were collected and dissociated to yield a cell suspension prior to lysis of red blood cells. Single cell libraries were prepared according to the protocol for 10X Genomics for 3' Gene Expression. Approximately 10,000 sorted CD45.1+ Va2+ CD4+ CD44+ cells were loaded and partitioned into Gel Bead In-Emulsions (GEM). scRNA libraries were sequenced on a HiSeq4000 (Illumina).
GEO Accession
GSM5345279