GSM1366260: HIP_7; Mus musculus; RNA-Seq
We identified pyramidal neurons by cell morphology, isolated single cells by aspiration using a wide-bore pipette, deposited collected material into an eppendorf tube and froze immediately at -20C until storage at -80C. Collected samples were individually amplified using three rounds of a linear in vitro-transcription-based method. Samples were prepared for multiplexed paired-end sequencing using the Illumina TruSeq v2 system according to manufacturer instructions.
GEO Accession
GSM1366260