GSM1582725: Mouse Input replicate 1; Mus musculus; ChIP-Seq
Testis samples were fixed with formaldehyde. The ChIP libraries were made from the input and the material immuniprecipitated with the Dmrt1 antibody described in PMID 11040213.   Chromatin fragments were blunted with Lucigen DNA terminator enyme mix (Lucigen# 40035).  Blunted fragments were adenylated with Exo- Klenow (NEB #MO2125).  Illumina adapters (Illumina #100521) were diluted 1:50 and ligated to adenylated fragments with T4 ligase (NEB #NEB M0202T).  Products were amplified with 18 rounds of PCR using Phusion Polymerase (NEB M0530s) using barcoded oligonucleotides.  DNA fragments between 150 and 250 were purified by agarose gel electrophoresis. ChIP-Exo libraries were prepared as described in PMID: 22153082.
GEO Accession
GSM1582725