GSM4721209: PSA-stimulated CD4+ T cells MD74; Mus musculus; RNA-Seq
Qiagen RNeasy RNA library is prepared according to Illumina's protocol1 and sequencing is conducted on Illumina HiSeq 2500. The 3' adaptor (TGGAATTCTCGGGTGCCAAGG) for sequencing is removed from the raw reads. After removing the 3' adaptor, reads with the length less than 10 nt are excluded. The remaining reads are aligned against genome using Tophat v1.4.12 and assembled using Cufflinks v2.2.13. The abundance is estimated in Fragments Per Kilobase of transcript per Million mapped reads (FPKM) as defined in Cufflinks. Differential expression analysis is conducted using the Cuffdiff module of Cufflinks and the q-value (False-Discover-Rate adjusted p-value) of 0.05 is used as the cutoff to define genes showing significant difference.
GEO Accession
GSM4721209