GSM3294933: 7dCI_rep2; Homo sapiens; RNA-Seq
Proliferating and 7dCI fibroblasts were lysed using 1 mL of Trizol reagent (Life Technologies, Carlsbad, CA) per 10 cm plate for 5 minutes.  RNA was isolated from Trizol lysates following manufacturer instructions. RNA concentrations were determined using a Nanodrop Spectrophotometer (Thermo Fisher Scientific Inc., Waltham, MA). RNA quality was verified on a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA) using reagents from the RNA Nano 6000 kit (Agilent Technologies). cDNA libraries were constructed using the Illumina TruSeq mRNA sample preparation kit according to the manufacturer's instructions for revision A of the protocol (Illumina Part #15008136).  The low-input protocol was followed for all samples and 1 to 10 μg of total RNA input was used per library.
GEO Accession
GSM3294933