RNA-Seq of Eupausia superba: head and carapace
TruSeq Stranded mRNA kit (Illumina, San Diego, CA) has been used for library preparation following the manufacturers instructions. RNA samples were quantified and quality tested by Agilent 2100 Bioanalyzer RNA assay (Agilent Technologies, Santa Clara, CA). Final libraries were checked with both Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA) and Agilent Bioanalyzer DNA assay and Caliper GX (PerkinElmer, Waltham, MA). Libraries were sequenced in paired-end 150 bp layout on NovaSeq 6000 (Illumina, San Diego, CA).