GSM5034385: ATAC-20K-exp2; Mus musculus; ATAC-seq
For low-input ATAC&mRNA-seq, harvested cells were washed and then permeabilized with mild detergent to facilitate the entry of Tn5 into the nuclei to tagment open chromatin regions. Tagmented cells were then lysed and Dynabeads Oligo (dT)25 were added into the cell lysate to capture mRNA. After magnetic separation, tagmented genomic DNA in the supernatant was purified and further amplified with indexed PCR to construct ATAC-seq library, while mRNA captured on beads was reverse transcribed using the bead-bound oligo (dT) as primer. The mRNA/cDNA hybrids were then directly tagmented by Tn5, and after initial end extension, the tagmented cDNA was amplified with indexed PCR to prepare mRNA-seq library using Nextera XT DNA Library Preparation Kit. Omni-ATAC-seq was performed following the Omni-ATAC protocol (Corces et al., 2017). Open chromatin regions and mRNA/cDNA hybrids were tagmented with Tn5 and then amplified with indexed PCR to prepare ATAC-seq and RNA-seq libraries.
GEO Accession
GSM5034385