GSM1365900: LS174T, H3K27ac ChIP-seq; Homo sapiens; ChIP-Seq
Cells were lysed in 0.5% SDS, lysates were sonicated and clarified. Chromatin was diluted 5 fold and incubated with beads for pre-clearing. After pre-clearing, chromatin was incubated o/n with anti-GATA6, anti-H3K4me3, anti-H3K4me1, or anti-H3K27ac antibodies. 10ng of DNA, as quantitated by fluorometry, was resolved by electrophoresis and fractions of 50-250bp were extracted. Fractions were processed through subsequent enzymatic treatments of end-repair, dA-tailing, and ligation to adapters as in Illumina's "TruSeq DNA Sample Preparation Guide" (part # 15005180 Rev. C). Adapter-ligated libraries were completed by limited-cycle PCR with Illumina PE primers (12 cycles). The resulting purified DNA library was applied to an Illumina flow cell for cluster generation (TruSeq cluster generation kit v5) and sequenced on the Illumina Hi-Seq 2000 with SBS TruSeq v5 reagents by following manufacturer's protocols.
GEO Accession
GSM1365900