GSM2633855: Corazonin receptor KD - rep1 (t2); Harpegnathos saltator; RNA-Seq
Brains (minus optic lobes) were dissected and RNA extracted with TRIzol. DNA was removed using Turbo DNase. Each brain was used to construct a separate library (biological replicate). RNA was fragmented by boiling in presence of divalent cations. RT was performed with random hexamers and dUTP was incorporated in the 2nd strand to retain strand information. Strand-specific RNA-seq (dUTP)
GEO Accession
GSM2633855