GSM4594199: Adult Intact and Castrated Mouse Prostate Run 1; Mus musculus; RNA-Seq
Cells were lysed in droplets in the 10x Genomics Chromium system. cDNA synthesis and library construction was performed using the 3' scRNA-seq Chromium Single Cell 3ʹ GEM Library & Gel Bead Kit v3 (10x Genomics). Gene expression (cDNA) libraries were prepared according to the manufacturer's protocol. MULTI-seq barcode libraries were separated from the cDNA libraries during the first round of size selection, and PCR amplified prior to sequencing according to the MULTI-seq library preparation protocol (McGinnis et al., 2019). RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM4594199