MNase-Seq of saccharomyces cerevisiae: Untagged Control Total nucleosomes with rapamycin: Sample No FRB tag (+RAP) Total nucleosomes
An Anchor Away strain without the FRB tag was grown to log phase and treated with rapamycin for one hour prior to fixation with paraformaldehyde. Mononucleosomes were released by MNase digestion and the nucleosome-associated DNA fragments were mapped by deep sequencing.