GSM5732760: 17_Tumor; Rattus norvegicus; RNA-Seq
Mammary tumor, mammary gland (MG) and muscle were harvested and snap frozen in liquid nitrogen. RNA was isolated from MG and muscle using Qiazol reagent and RNeasy mini kit (Qiagen, Inc., USA). Mammary tumor RNA was isloated using the RNeasy mini-kit (Qiagen, Inc.) with addition of a proteinase K digestion step for 10 min at 55 °C between the lysis buffer and ethanol steps. RNA libraries were prepared for sequencing using standard Illumina protocols (Nugen Universal mRNA kit, Tecan Genomics, Inc., Redwood City, CA, USA)