GSM4721948: Im-GCT-4072__M101__2B2__G34W-unedited__snRNAseq; Homo sapiens; RNA-Seq
Fresh tumours collected after surgery were dissociated in Collagenase-Dispase (Sigma-Aldrich). Red blood cells were lysed by ammonium chloride treatment for 5 min on ice. Cell viability was assessed with Trypan Blue. For samples with low viability (<60%), dissociated cells were enriched for live cells using Dead Cell Removal kit (Miltenyi Biotech). 10,000 dissociated cells per sample were loaded on the 10X Genomics Chromium controller. The Chromium Single Cell 3′ (10X Genomics, Version 3) protocol was strictly followed to prepare libraries. The 10X libraries were sequenced on Illumina NovaSeq 6000 S4 platforms.
GEO Accession
GSM4721948