GSM1363041: 132vvs; Bos taurus; RNA-Seq
Day 7 and Day 10 blastocysts were pooled into groups of five, while individual conceptuses from Days 13, 16, and 19 were used for mRNA extraction. Polyadenylated mRNA was isolated from Day 7, Day 10, and Day 13 blastocysts/conceptuses by two rounds of selection using the Dynabeads mRNA DIRECT Kit (Invitrogen). However, for Day 16 and Day 19 conceptuses, initially total RNA was isolated using the TRIzol reagent (Invitrogen) per the manufacturer’s instructions. On-column DNase digestion and RNA cleanup was performed using an RNeasy Midi Kit (Qiagen) followed by two rounds of polyadenylated mRNA selection using Dynabeads mRNA DIRECT Kit (Invitrogen). Each developmental stage was represented by five biological replicates that were processed independently. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM1363041