GSM5597814: K2; Mus musculus; RNA-Seq
Total RNA was extracted from FACS-purified MuSCs with Nucleospin RNA XS kit (Machery-Nagel) RNA was reverse transcribed using oligo(dT) primers with the SMARTer Ultra Low Input system (Takara). The cDNA was then sheared with a Covaris S2 ultrasonicator. End repair, multiplexed adapter ligation and 13–15 cycles of library amplification were performed using the Ovation Ultralow Multiplex system (NuGEN)
GEO Accession
GSM5597814