MNase-Seq of saccharomyces cerevisiae: RPB1-FRB H2A nucleosomes with rapamycin: Sample RPB1-FRB (+RAP) AA nucleosomes
"The Anchor Away strain RPB1-FRB was grown to log phase and treated with rapamycin for one hour prior to fixation with paraformaldehyde. Mononucleosomes were released by MNase digestion and FLAG epitope-tagged H2A.Z-containing nucleosomes were quantitatively immunoprecipitated using anti-FLAG agarose and eluted using FLAG peptides. The flow-through, enriched for H2A nucleosome-associated DNA fragments, was mapped by deep sequencing."