Subread of Alsophila spinulosa Rachis in FL RNA-Seq
The mRNA was enriched by Oligo dT beads and reverse transcribed into cDNA through the SMARTer PCR cDNA Synthesis Kit. Then the > 4 kb cDNA were partitioned by BluePippin Size Selection System and amplified using PCR. The unfiltered cDNA and > 4 kb cDNA fractions were mixed with a mole ratio of 1:1. Then the mixed cDNA was used for preparing the size-fractionated SMRTbell library by the SMRTBell Template Prep Kit. Finally, the library was sequenced on a single Sequel II System SMRT Cell.