Cytoplasmic Male sterility
Cytoplasmic male sterility (CMS) is caused by rearrangement of the mitochondrial genome, and a novel chimeric open reading frame (ORF) created by shuffling of endogenous sequences is often responsible for CMS. Sequence comparison of Mitochondrial DNA of CMS line and Fertile line of Allium cepa would lead to identification of novel ORFs, linked male sterility. The mitochondrial DNA has been isolated from CMS line- 48A of allium cepa using sucrose gradient and the library consist of 100-300 base reads.