Sequences of CLTA, EMX and VEGF on- and off-target genomic sites from Hek293T cells treated with Control-nickase Cas9and control sgRNAs
Sequencing from Guilinger, Thompson & Liu. Nature Biotechnology 2014.  The target site and its corresponding known off-target sites of CLTA, EMX, and VEGF were amplified from genomic DNA isolated from HEK293 cells treated with Cas9 nickase and two sgRNAs targeting an unrelated site (GFP) as a negative control.  All genomic target sites (for CLTA, EMX, and VEGF) were combined together forthis one type of Cas9 treatment.