RNA-seq of Apis mellifera:hypopharyngeal glands
Total RNA was extracted from each sample using TriReagent (Invitrogen) according to the manufacturers specifications. RNA integrity was confirmed using Agilents 2100 Bioanalyzer. 4 lg of total RNA from each the six RNA sequencing (RNA-Seq) libraries were prepared using Illuminas TruSeq RNA Sample Preparation Kit. The sequencing libraries were validated and run through a 6% TBE PAGE gel. 50 bp fragments were isolated and run through the Illumina HiSeq 2000/2500 sequencer for 2  150 cycles at a starting concentration of 12 pM per library. All six samples were run on one lane.