GSM5171016: Library 1 ref rep5; Escherichia coli; OTHER
plamids exctrct from E coli Plasmids were used as a template for a PCR using primers targeting the common sgRNA scaffold and containing Illumina TruSeq adapter sequences, with the following parameters: 98ºC 5', 30 cycles of (98ºC 30'', 58ºC 30'', 72ºC 30''). The number of cycles was optimized by DNA quantification to stop in the middle of the linear amplification phase. Illumina i5 and i7 adapters were added by performing an additional 10-12 PCR cycles with TruSeq adapter primers
GEO Accession
GSM5171016