mice microbiota
Total genome DNA from samples was extracted using The Hipure Soil DNA Kit (Magen, Guangzhou, China) according to the manufacturers protocols. DNA concentration was monitored by Qubit3.0 Fluorometer. 20-30 ng DNA was used to generate amplicons. Hypervariable V3/V4-16S rDNA were selected for generating amplicons with primers (F: CCTACGGRRBGCASCAGKVRVGAAT; R: GGACTACNVGGGTWTCTAATCC) and following taxonomy analysis. Sequencing was performed using PE250/300 paired-end in the MiSeq instrument.