GSM3455901: SGC_7901_DDP; Homo sapiens; RNA-Seq
Total RNA for RNA sequencing (RNA-seq) was isolated from SGC7901 cells with different treatment. After extracting from cells, total RNA was quantified and integrity measured by BioAnalyzer 2100. Oligo (dT) magnetic beads were used to select mRNA with polyA tail and the target RNA was obtained after purification. Fragmented the target RNA and reversed transcription to double-strand cDNA (dscDNA) by N6 random primer. Then End repair was performed. The dscDNA was ligated with adaptor and amplified by PCR. Denatured the PCR product by heat and the single strand DNA is cyclized by splint oligo and DNA ligase.
GEO Accession
GSM3455901