Hypsiglena unaocularus CNAR-IBH 28127 complete mt-genome KJ486458
Complete mitochondrial genome, first amplified with long PCR in two pieces, "a" from ND4-16S, then "b" from 16Sto ND4.  Halves were kept in separate libraries, sheared, tagged, and multiplexed on PGM Ion Torrent.  Advanced snakes have two, nearly identical control regions, thus they were captured and maintained in separate libraries until final assembly and annotation.