GSM2631748: SKOV_FAV_pcDNA3; Homo sapiens; RNA-Seq
cDNA library produced with retroviral RT The poly(A) RNA was isolated and purified from 5ug DNA-free total RNA using NEBNext Poly (A) mRNA Isolation Module (New England Biolabs) according to the manufacturer protocol. Library preparations were performed using the NEBNext Ultra directional RNA library Prep Kit for Illumina (New England Biolabs) in order to generate RNA-seq library from 100 ng of purified RNA. The RNA and library quality were verified using Agilent Bioanalyzer (Agilent Technologies).
GEO Accession
GSM2631748