GSM3293810: AB1Lysates_20min_rep1; Acinetobacter phage AB1; RNA-Seq
Total RNA was isolated using SV Total RNA Isolation System (Promega) according to the manufacturer's instructions and   checked for a RIN number to inspect RNA integration by an Agilent Bioanalyzer   2100 (Agilent technologies, Santa Clara, CA, US).Qualified total RNA was further   purified by RNeasy mini kit (Cat#74106, QIAGEN, GmBH, Germany) and   RNase-Free DNase Set    (Cat#79254, QIAGEN, GmBH, Germany). Barcoded sequencing libraries Pooled libraries were enriched for the exome using hybridization (TruSeq Exome Enrichment Kit, Illumina), quantified using quantitative PCR and pooled in 1:1 ratio for pairedend sequencing on a HiSeq2500 (Illumina)
GEO Accession
GSM3293810