shotgun sequencing of porcine fecal matter : 12 weeks old pig
Genetic material was extracted from the 0,22um-filtrated supernatant of centrifuged fecal homogenate with a Qiagen viral RNA mini kit, reverse-transcribed with a second strand synthesis and randomly amplified by PCR. Libraries were prepared with a Kapa HTP Library preparation kit with Nxtflex-96 DNA Barcodes.