Meta-transcriptome of Montipora capitata: MC-289_T3-Amb_1595
All M. capitata nubbins were collected and glued on 7-8 May 2019. The nubbins were left to acclimate until the study started on 22 May 2019. Samples were held at 27.67C (mean) for the remainder of the experiment. Sample was collected on 3 June 2019. At the time of collection, each nubbin was transferred to a Whirl-pak under water, the water was drained from the Whirl-pak, and then flash-frozen in liquid nitrogen. The Qiagen AllPrep DNA/RNA/miRNA Universal Kit was used to isolate RNA, the TruSeq RNA Sample Preparation Kit v2 (Illumina) was used to prepare strand specific cDNA libraries. The protocol included poly-A selection; quality control was done using an Agilent Bioanalyer and a Thermo Fisher Qubit dsDNA BR (Broad Range) assay kit.