GSM1370373: NoDice(2-20) Total Small RNA; Homo sapiens; miRNA-Seq
TRIzol total RNA isolation followed by electrophoresis on a 15% polyacrylamide TBE-urea gel.  Small RNAs ~15-40nt in length were identified using radiolabeled markers and electroeluted from the gel slice Constructed with a protocol analogous to the illumina TruSeq small RNA.  RNA adapters identical to the illuman adapters were ligated overnight with 3' adapter-ligated small RNAs purified by gel electrophoresis on a 10% TBE-urea acrylamide gel and electroeluted in lieu of the “stop” solution.  Reverse transcription was performed with Invitrogen SuperScript III and PCR amplification using Promega's GoTaq Green Master Mix
GEO Accession
GSM1370373