Deciphering the diversity of human astrovirus and human norovirus in wastewater effluents
The goal of this study was to monitor the seasonality of human astrovirus and norovirus in WWTP effluents by high throughput sequencing. One specific MID was selected in order to discriminate each season (Spring: ACGCTCGACA; Summer: AGACGCACTC; Autumn: AGCACTGTAG; Winter: ACGAGTGCGT); Human astrovirus primers (Forward primer 5'-3': CAACTCAGGAAACAGGGTGT; Reverse primer 5'-3': ACCAATGACAATGCATCTGA); Human NoV GI (Forward primer 5'-3': CTGCCCGAATTYGTAAATGA; Reverse primer 5'-3': TGTAYAATGGYTGGGTTGG); Human NoV GII (Forward primer 5'-3': CNTGGGAGGGCGATCGCAA; Reverse primer 5'-3': ATGTAYAAYGGDYATGCNGGYGG)