GSM2631905: S12110100; Homo sapiens; RNA-Seq
Total RNA was extracted from flow cytometry-sorted microglia cells using an RNeasy and AllPrep Micro Kit (Qiagen) and from whole brain tissue using RNeasy Lipid Tissue Mini kit (Qiagen), according to the manufacturer's protocol. Illumina TruSeq RNA Sample Prep Kit was used with 60 ng of total RNA for the construction of sequencing libraries of 23 samples. Clontech SMARTer Stranded Total RNA-Seq Kit - Pico Input (Takara Bio, Japan) cDNA libraries were prepared for 16 samples starting with 500 pg of total RNA. Human RNA libraries were prepared for sequencing using standard Illumina and Clontech protocols. Mouse  RNA samples were prepared with a Quantseq 3'mRNA-Seq kit. (Lexogen, USA)