HIV Minor Variant Analysis in PrEP Seroconverters
This sequence data resulted from sequencing amplicons defining the HIV reverse transcriptase gene. HIV RNA was extracted from each sample, cDNA synthesis and first round amplification performed, followed by PCR to generate a unique HIV reverse transcriptase gene-specific amplicon for each sample by incorporation of 10-base multiplex identifier sequences. Sequencing libraries were created by pooling the unique amplicons generated for each sample. The resulting data for each run was de-multiplexed based on the unique combinations of 10-base sequence tags assigned to each sample, mapped to the HIV genome, and subsequently analyzed for HIV drug resistance variants.