GSM4816478: F6LHplus7; Homo sapiens; RNA-Seq
Endometrial tissue biopsies were obtained with the use of a Pipelle, immediately after UF sampling. Uterine fluid samples were obtained by lavage of the endometrial cavity with 2.5 of physiologic solution using a balloon hysterosonosonography catheter to avoid vaginal contamination. Extracellular vesicles were isolated- after mucus disgregation by vortexing- using standard ultracentrifugation protocol.  RNA was harvested using  RNase micro KIT of QIAGEN with DNAse treatment step in coloumn. To compare the transcriptomic profile of tissues and UF-Evs (samples 1-20), cDNA libraries were generated from total RNA using the QuantSeq 3' mRNA-Seq Library Prep Kit (Lexogen GmbH, Vienna, Austria). To perform transcriptome sequencing on UF-EVs collected from fertile women and ART patients during the window of implantation (samples 21-93), cDNA libraries were prepared using the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio Inc., Otsu, Shiga, Japan)
GEO Accession
GSM4816478