GSM4404717: bzip60-DMSO-12h_1; Arabidopsis thaliana; RNA-Seq
Plants germinated and were subjected to the ER stress recovery assay as described above. Whole seedlings were harvested at 0 h, 12 h and 24 h of recovery in three biological replicates (n = 12 per each replicate), and immediately frozen in the liquid nitrogen. The frozen samples were ground to a fine powder in liquid nitrogen using a Retch MM400 Mixer Mill with zirconium oxide balls. Total RNA was extracted using the NucleoSpin RNA Plant kit (MACHEREY-NAGEL, Düren, Germany) according to the manufacturer's instruction. RNA-seq libraries were constructed using the Illumina TruSeq Stranded mRNA Library (Illumina, San Diego, CA, USA)
GEO Accession
GSM4404717