GSM2633611: NucRNAseq_FTY2; Mus musculus; RNA-Seq
Populations of 15,000 DAPI+NeuN-GFP+ nuclei were directly sorted into an extraction buffer for RNA isolation of PicopureTM RNA isolation kit (Arcturus, ThermoFisher). Deoxyribonuclease I (DNase I) (Qiagen) was used to remove genomic DNA. A sequencing library was produced using 0.75 ng of the amplified cDNA in the Nextera XT Library Preparation Kit (Illumina).
GEO Accession
GSM2633611