GSM5224952: 4-1R-sham-gwat; Mus musculus; RNA-Seq
Adipose tissue was isolated from mice and immediately flash frozen in liquid nitrogen. Samples were then stored at -80oC. RNA was subsequently isolated using the QIAGEN RNeasy Plus Mini Kit (Qiagen #74134). Briefly, we added 350 ul of RLT Buffer supplemented with 3.5 ul β-mercaptoethanol (Sigma #M3148), and then crushed the tissue in a 1.5 mL tube using a plastic pestle. Samples were sequentially transferred to QIA Shredder columns, genomic DNA eliminator columns, and RNA isolation columns. RNA was eluted using 50 ul of nuclease-free water. RNA libraries were prepared for sequencing at Genewiz using standard Illumina protocols
GEO Accession
GSM5224952