RNA-Seq of Rhinella marina: adult female brain
RNA was extracted using the RNeasy Lipid Tissue Mini Kit (QIAGEN, USA) following the manufacturer's instructions, in conjunction with a genomic DNA removal step (QIAGEN, USA); mRNA libraries were constructed using the TruSeq mRNA v2 sample kit (Illumina, USA), including a 300 bp selection step; capture of mRNA was performed using the oligo dT method, and size selection parameter choices were made according to the HiSeq2500 manufacturer's protocol (Illumina).