GSM2632098: hMuStem_HS_3; Homo sapiens; RNA-Seq
Total RNA was extracted using RNeasy mini or micro kit following manufacturer’s instructions. Libraries were prepared from 10 ng of total RNA. The mRNA poly(A) tail was tagged with universal adapters, well-specific barcodes and unique molecular identifiers (UMIs) during template-switching reverse transcriptase. Barcoded cDNAs from multiple samples were then pooled, amplified and tagmented using a transposon-fragmentation approach that enriches for 3’ends of cDNA. A library of 350-800 bp was run on an Illumina HiSeq 2500 using a TruSeq Rapid SBS Kit (Illumina, San Diego, CA, USA).
GEO Accession
GSM2632098