pVPSBA01-truncated NSP1-rep3
Briefly, 1e6 H1299 cells were electroporated with 3 g Nsp1, mutant Nsp1, and relative control plasmids. Cells were collected 24hr post electroporation. Total mRNA was extracted with RNasy Plus Mini Kit (Qiagen). 1g total mRNA each sample was used for the RNA-seq library preparations. A NEBNext Ultr RNA Library Prep Kit for Illumina was employed to perform RNA-seq library preparation and samples were multiplexed using barcoded primers provided by NEBNext Multiplex Oligos for Illumina (Index Primers Set 1).