Genome sequencing of Thermus filiformis ATT43280 - Mate pairs
T. filiformis was cultivated in Petri dishes containing the solid medium Castenholz TYE 1 % at 70 °C for 24 h (Ramaley and Hixson, 1970). Then, part of the culture from the Petri dishes was transferred to a 250 mL Erlenmeyer flask containing 30 mL of Castenholz TYE 1% and incubated on a shaker at 230 rpm for 24 h at 70 C. The suspensions of cells were centrifuged at at 8,000 g for 10 min at 10C, the supernatant discarded and the pellet was used for DNA extraction.  The genomic DNA of Thermus filiformis was extracted with the phenol/chloroform method and then purified with PowerClean® DNA Clean-Up Kit (MoBio laboratories, USA). After that, one library for sequencing was prepared using Nextera Mate Pair gel-free protocol . That library was sequenced using the Illumina MiSeq Sequencing system (MiSeq Reagent Kit v2 of 500 cycles) with 250 base pair end chemistry (250x2bp). The sequencing was performed at the facility of the Brazilian Bioethanol Science and Technology Laboratory (Campinas, Brazil).