RNA-Seq of S. haematobium: Mixed-sex adult worms ONT RNA002
Messenger RNA isolated from mixed-sex adult worms was used to prepare Oxford Nanopore direct RNA-sequencing library using the SQK-RNA002 kit, according to the manufacturers instructions.  All libraries were sequenced on a MinION sequencer (Oxford Nanopore Technologies, Oxford, UK) for 48-72 hours (i.e. until no more active pores were available), using an EXP-FLP002 flow cell priming kit and three R9.4.1 flow cells (FLO-MIN106). Reads were then base-called from raw fast5 files using a GPU-enabled version of the program Guppy v.3.2.4 and providing the configuration file rna_r9.4.1_70bps_hac.cfg. Reads that did not pass the quality filter within Guppy (Q  7) were removed