GSM5774290: P2_L3_F; Mus musculus; RNA-Seq
RNA was extracted from cardiomyocytes by homogenising samples in QIAzol Lysis Reagent and performing column-based purification of RNA using miRNeasy Mini Kit (Qiagen). Sequencing libraries were constructed from 1 µg of total RNA using stranded TruSeq total RNA library preparation kit (Illumina) Libraries were sequenced on an Illumina NovaSeq 6000 aiming for a depth of 20 million 1x100bp single end reads per sample to generate RNA-Seq datasets.