Transcriptome of tea plants respond to early cold stress
Using RNA-Seq, DGE and sRNA-Seq technologies, we performed an integrative analysis of miRNA and mRNA expression profiling and their regulatory network of tea plants under chilling (4 degree) and freezing (-5 degree) stress.  Leaf samples from 10 tea plants were collected for RNA preparation. Total RNA was isolated using a TRIzol reagent (Gibco BRL) following the manufacturer's instructions, and then beads with Oligo (dT) were used to isolate poly (A) mRNA. After fragmentation, random hexamer-primer was used to synthesize the cDNA. Short cDNA fragments were purified and followed by end reparation, adenylation and sequencing adapter ligation. After PCR amplification of the selected fragment, the library was sequenced using Illumina HiSeq 2000.