GSM3294594: H3K4me3_3; Homo sapiens; ChIP-Seq
20 million cells/replicate were fixed using 1% formaldehyde for 10 minutes at 24C, quenched for 5 minutes with 0.125M glycine, and stored at -80C until use. Pellets were then thawed on ice and lysed using 1% SDS_lysis buffer, sonicated for 27 cycles (30on/30ff) using a temperature controlled bioruptor, and spun down at max speed for 10 minutes.  Clarified chromatin was then incubated with primary antibody overnight, and genomic DNA washed and isolated the next morning. ChIP sequencing libraries (20ng DNA/sample) were constructed using the KAPA Hyper Prep Kit (Illuminia- Kapa biosystems Cat # KK8502, NimbleGen SeqCap Adapter Kit A- Roche Cat # 07 141 530 001) according to the manufactures instructions.  Libraries were assessed for quality, purity, and size using DNA High Sensitivity Bioanalyzer chips, and those passing QC were quantified using the Library Quantification Kit from Illiumina (Kapa Biosystems KK4854).  Libraries were pooled to a final concentration of 10nM and sequenced using the Illumina HiSeq 4000
GEO Accession
GSM3294594