GSM4816669: SI-NET-seq K562 dTAG7 2h R1; Homo sapiens; OTHER
Nascent RNA was extracted as described by Mayer and Churchman (2016) with the following modification: After treatment of MOLT4 or K562 dTAG-BRD4, NIH3T3 cells were added in a ratio of 1:6. Libraries were constructed as described by Mayer and Churchman (2016). In case of the libraries on K562 dTAG-BRD4,  the following modifications were implemented: After purification of the nascent RNA, a DNA linker containing a random decamer sequence was ligated to the 3'-end of the transcripts. Ligated RNA was fragmented by incubation with NEBNext RNA fragmentation solution at 95°C for 10 min. To improve read mapping and take the increased linker length into account, all size selection steps were adapted.
GEO Accession
GSM4816669