4C-Seq of homo sapiens: TFF1 enhancer viewpoint R1 in MCF7_TFF1pKO
The 4C (circular chromatin conformation capture) sequencing data studied the chromatin interactions stemming from one genomic region of interest, which was called viewpoint. In this sample, the viewpoint was based on an enhancer in the KCNK5 gene locus. The experiments were conducted followed a published protocol(van de Werken et al., Nature Method, 2012). Briefly, cells were crosslinked by formaldehyde, digested by DpnII enzyme, then subjected to in nuclei ligation by T4 ligase. The cell genomic DNA was then extracted, further diegsted with a second enzyme NlaIII, subjected to cirulization and finally PCR amplification of target region.