NextSeq 550 sequencing; scRNA-seq of sea lamprey (Petromyzon marinus) brain regions
scRNA-seq of sea lamprey (Petromyzon marinus) brain regions
Tissue samples were dissected from wild caught specimens. Sampled animals were euthanized by submersion in 0.1% MS-222 (Sigma, A5040-25G), unless specified otherwise, followed by decapitation according to local guidelines.  Cells were isolated through a combination of mechanical and chemical lysis. Per library 15 000 – 20 000 nuclei were loaded into one channel of the ChromiumTM Single Cell A Chip (10X Genomics®). cDNA amplification and scRNA-seq libraries were constructed with Single-Cell 3′ Gel Bead and Library v2 (for larvae) and v3 Kit (for adults), following the instructions of the manufacturer.
Experimental Factor: developmental stage
adult, mature
Experimental Factor: organism part
rhombencephalon