GSM2632116: WT-LN-Treg-Biological-Replicate3; Mus musculus; RNA-Seq
Superficial and deep axillary LNs were isolated and homogenized in complete DMEM followed by FACS staining for sorting of Tregs Total RNA was extracted with Pico pure kit (Life technologies) followed by DNase treatment with the RNeasy kit (Qiagen). The SMART-Seq v4 Ultra Low Input RNA Kit (Clontech) was used according to the manufacturer's instructions and 75 bp single-end reads were sequenced on an Illumina NextSeq 500 instrument.
GEO Accession
GSM2632116