Differential gene expression Nanomia bijuga
Aim of the study was to obtain insights into differential gene expression between functionally specialized              polyps (gastrozooids) and medusae (nectophores) of siphonophore Nanonima bijuga (Cnidaria). Young nectophores and gastrozooids were dissected              from three different animals (1-3), generating three paired tissue samples. mRNA was extracted directly from tissue (New England Biolabs, #S1550S)             and sequencing libraries were generated using the Illumina mRNAseq protocol.The three biological replicates of each tissue type were sequenced in              6 lanes on a Genome analyzer IIx. g specifies a gastrozooid sample, n specifies a nectophore sample. Numbers in library name indicate the sampled             Nanomia specimen (1-3).
Illumina mRNA-seq sample kit (#RS-930-1001, Illumina Inc.)