MNase-Seq of saccharomyces cerevisiae: INO80-FRB Total nucleosomes with rapamycin: Sample INO80-FRB (+RAP) Total nucleosomes
The Anchor Away strain INO80-FRB was grown to log phase and treated with rapamycin for one hour prior to fixation with paraformaldehyde. Mononucleosomes were released by MNase digestion and the nucleosome-associated DNA fragments were mapped by deep sequencing.