Environmental Genomics of an Antarctic Polychaete
Adult worms were pooled into 4 samples with 25 individuals per sample. Each sample was prepared for standard Illumina NGS fragment library sequencing. Each sample was sequenced in a single lane of a HighSeq2500 flow cell. 2 samples used worms cultured at a normal temperature of -1.5C for 28 days. 2 samples used worms cultured at +4.0C as a thermal stress. This species is endemic to Antarctica and its upper thermal tolerance is ~6C.