GSM5082612: VDR-cKO microglia rep 4; Mus musculus; RNA-Seq
Total RNA was extracted from microglia isolated from the ischemic brains of VDR-cKO and VDRflox mice using TRIzol reagent. Total RNA from each sample of sufficient quality, defined as RNA integrity number (RIN) > 7.0, was included for subsequent analysis. A total amount of 1 μg RNA per sample was used. In brief, the NEBNext rRNA Depletion Kit was used to remove rRNA from the total RNA. The NEB Next Ultra RNA Library Prep Kit for Illumina (NEB, Beijing, China) was used to construct the libraries for sequencing following the manufacturer's instruction.
GEO Accession
GSM5082612