GSM5034270: RNA-Seq anti_miR-340-5p_2; Homo sapiens; RNA-Seq
RNA was isolated from cells using the TriPure isolation reagent (Roche) and the Direct-zol Mini Kit (Zymo) following the manufacturers' protocols. The total RNA purity and quantity were analyzed by RNA 6000 Nano LabChip Kit (Agilent, Palo Alto, CA, USA) and Bioanalyzer 2100 with RIN number >7.0. About 1 μg of RNA was used to construct small RNA library according to the protocol of Illumina's TruSeq small RNA sample preparation Kits (San Diego, CA, USA). Sequencing was performed on Illumina HiSeq2500 with single-end mode (36bp SE). 
GEO Accession
GSM5034270