GSM1582869: K2; Rattus norvegicus; RNA-Seq
Total RNA from was extracted, DNase-treated, and purified (RNeasy, Qiagen). 2µg of total RNA underwent quality control (Bioanalyzer; all RIN values > 9.5) and library construction for polyA+ or polyA- RNA sequencing. For PolyA- RNA, an additional step (removal of ribosomal RNA with NEBNext rRNA depletion kit) occured prior to adapter ligation. Directional polyA+ and polyA- RNA llibraries were constructed at HudsonAlpha Genomic Services Lab using NEBNext reagents (New England Biolabs) according to manufacturer's recommendations with minor modifications (including the use of custom library adapters and indexes). RNA libraries were quantified (Kapa Library Quant Kit, Kapa Biosystems), and underwent sequencing (25M total 50bp paired-end reads) on an Illumina sequencing platform (HiSeq2000).
GEO Accession
GSM1582869