RNA-seq of mus musculus: IRTKS wildtype MEF cells
The mRNAs were separated by the NEBNext Poly(A) mRNA Magnetic Isolation Module based on the coupling of Oligo d(T)25 to paramagnetic beads which was then served as the solid support for the direct binding of poly(A)+ RNA. Sequencing libraries were generated using NEBNext Ultra Directional RNA Library Prep Kit for Illumina according to the manufacturers instructions, and paired-end sequenced by Illumina HiSeq-4000.