GSM5034639: E12.5 inv2 distal forelimb capture Hi-C; Mus musculus; Hi-C
The forelimbs of embryos were dissected and placed into PBS with 10% FCS and 8ul collagenase at 50mg/ml (Sigma C9697) at 37º for approximately 5min. Collagenase treated samples were cross-linked with 1% formaldehyde (ThermoFisher 28908) for 10 minutes at room temperature and stored at -80º until further processing as previously described (Yakushiji-Kaminatsui et al., 2018) Following the Hi-C protocol, ligated DNA fragments were processed throught the Agilent SureSelectXT protocol to enrich for fragments coming from the probe region chr2:72240000-76840000 (mm9). SureSelectXT
GEO Accession
GSM5034639