GSM1366944: oocyte_RNA-Seq; Mus musculus; RNA-Seq
Metaphase II oocytes were collect by removing all somatic contaminants. Sample for RNA isolation was collected in Buffer RLT (RNeasy Micro Kit from QIAGEN). The RNA-seq libraries were constructed as described (Tang et al., 2010). Briefly, reverse transcription was carried out at the first step. Then, free primers were removed by exonmulease I and a polyA tail was added to the 3' end of the first-strand cDNAs by terminal deoxynucleotidyl transferase. The cDNAs were amplified by PCR and subjected to construct a sequencing library sequenced by Illumina HiSeq 2000.
GEO Accession
GSM1366944