GSM1369970: siAGO1_MCF7_rep2; Homo sapiens; RNA-Seq
Illumina protocol. Using 3.5 ug of total RNA to isolate polyA+ mRNA using Sera magnetic beads coated with oligodT. Isolated mRNA was fragmented by incubation in buffered zinc solution and heat treatment. cDNA was generated by first creating a single-stranded cDNA using SuperScript II reverse transcriptase by random priming. Fragments corresponding to an insert size around 250bp were excised from the gel. Library was amplified with a 16-cycle PCR reaction, and the quality of libraries confirmed in the Agilent Technologies 2100 Bioanalyzer. Samples were sequenced on the HiSeq2000 using v1 sequencing chemistry using a paired end sequencing recipe ( 2 x 50 bases).
GEO Accession
GSM1369970