HPV DNA methylation of contiguous CpG sites by bisulfite treatment and massively parallel sequencing- the FRAGMENT approach: Human papillomavirus type 16 - sample 5
We chose 12 random samples, amplified multiple segments in the HPV16 genome with specific barcodes, inspected the methylation ratio in 31 CpG sites for all samples using Illumina sequencing, and compared the results with quantitative pyrosequencing.