Structural variations of 4 human cancer samples were identified by long span paired-end sequencing
LMP CAP adaptors with only a single 5’ phosphorylated end were ligated to the hydro-sheared DNA, thus creating a nick on each strand after circularization of the DNA. Both nicks were translated >50 bp into the circularized genomic DNA fragment by DNA polymerase I, and paired-end tags of >50 bp were released by T7 exonuclease and S1 nuclease. SOLiD sequencing adaptors P1 and P2 were ligated to the paired-end tag (PET) constructs, which were then amplified by PCR and sequenced by the Applied Biosystems SOLiD system.