16S rRNA gene amplicon sequencing of mainstream partial nitritation activated sludge bioreactors: timeseries/BONCAT, experimental SBR, mixed liquor, FastDNA extracted
16S rRNA gene fragments were amplified using barcoded 515F (5'-GTGYCAGCMGCCGCGGTAA-3') and 926R (5'-CCGYCAATTYMTTTRAGTTT-3') primers (Parada et al., 2016) targeting the V4-V5 hypervariable region of the 16S rRNA gene. Amplified barcoded PCR products were pooled at equimolar concentrations and sequenced on an Illumina MiSeq in paired-end 300bp mode.