GSM5184294: Total RNA (42 hpf); Strongylocentrotus purpuratus; RNA-Seq
Adult S. purpuratus were obtained from Pat Leahy (Caltech). Gametes were collected by intracoelomic injection of 0.5 M KCl. Fertilization and embryo culture were carried out according to Adams et al. (2019). Embryos were cultured at 15° C in 4-liter plastic beakers fitted with battery powered stirrers. Embryos were harvested at 0, 6, 12, 18, 24, 30, 36, 42, and 48 hr post- fertilization (hpf). The 9 samples were derived from two separate embryo cultures. For the 0 hpf time point, embryos were collected immediately after fertilization. Total RNA (75-100 g/sample) was isolated using the RNeasy Plus Mini kit (Qiagen Cat. No. 74134) and the quality of the preparations was confirmed using an RNA TapeStation (Agilent). RNA integrity numbers (RINs) for all samples were 9.8-10.0. RNA samples were stored at -80°C. CAGE library preparation, sequencing, mapping, and eRNA identification were performed by DNAFORM (Yokohama, Kanagawa, Japan)
GEO Accession
GSM5184294