GSM4404141: NUP98-KDM5A_AML N5A ChIP replicate 2; Mus musculus; ChIP-Seq
Fixed cell pellets were sonicated in Bioruptor for 45 cycles at high setting to enrich the sonicated DNA to be ~300-500bp. Then these sonicated samples were incubated with indicated antibodies with either protein G or A beads overnight. Then, the beads were washed 4 times with distinct buffers (see our method) and eluted with elution buffer at 65 degree for 30 min. This eluted DNA were reverse crosslinked and treated with RNase and protease K, then the final Chipped DNA are purified with PCR cleanup kit (Qiagen). The ChIP DNA library was prepared following the instructions of Illumina's ChIP-seq prep product manual. ChIP-seq libraries were sequenced on Illumina Genome Analyzer for sequencing.
GEO Accession
GSM4404141