MNase-Seq of saccharomyces cerevisiae: INO80-FRB H2A.Z nucleosomes with rapamycin: Sample INO80-FRB (+RAP) Z nucleosomes
"The Anchor Away strain INO80-FRB was grown to log phase and treated with rapamycin for one hour prior to fixation with paraformaldehyde. Mononucleosomes were released by MNase digestion and FLAG epitope-tagged H2A.Z-containing nucleosomes were quantitatively immunoprecipitated using anti-FLAG agarose and eluted using FLAG peptides. The IP fraction, enriched for H2A.Z nucleosome-associated DNA fragments, was mapped by deep sequencing."