RNA-Seq of Nt. root: bio-control agents treatments
Total RNAs were extracted from roots by using the Rneasy plant Mini Kit n according to the manufacturers manual, and mRNAs were purified by using poly-T oligo-attached magnetic beads. The concentration, purity, and integrity of RNA were assessed by using a NanoDropTM spectrophotometer (Thermo Scientific, USA) and an RNA 6000 Nano Kit with the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). The TruSeq RNA library preparation kit (Illumina, San Diego, CA, USA) was used to generate RNA libraries, and the quality was assessed on an Agilent Bioanalyzer 2100 system. Thereafter, all the  libraries were eventually constructed and were deep sequenced on the Illumina HiSeq 4000.