Sequences of CLTA, EMX and VEGF on- and off-target genomic sites from Hek293T cells treated with nickase Cas9and target sgRNAs
Sequencing from Guilinger, Thompson & Liu. Nature Biotechnology 2014.  The target site and its corresponding known off-target sites of CLTA, EMX, and VEGF were amplified from genomic DNA isolated from HEK293 cells treated with Cas9 nickase and two sgRNAs spaced 19 bp apart targeting the CLTA site, two sgRNAs spaced 23 bp apart targeting the EMX site, two sgRNAs spaced 14 bp apart targeting the VEGF site.  All genomic target sites (for CLTA, EMX, and VEGF) were combined together forthis one type of Cas9 treatment.