GSM5082332: IFNAR1 mutant, replicate 3; Mus musculus; RNA-Seq
Total RNA was extracted from 1-2 million PMN-MDSC using the Direct-zol RNA Miniprep (Zymo Research, Irvine, CA). RNA quantity was determined using the Qubit 2.0 Fluorometer (ThermoFisher Scientific, Waltham, MA) and the quality was validated using the TapeStation RNA ScreenTape (Agilent, Santa Clara, CA). 75 ng of DNAse I treated, total RNA was used to prepare library for Illumina Sequencing using the Quant-Seq 3'mRNA-Seq Library Preparation Kit (Lexogen, Vienna, Austria)
GEO Accession
GSM5082332