RNA-seq of mus musculus: colon tumoroids
NGS library construction was performed using the KAPA RNA HyperPrep Kit with RiboErase (KAPA Biosystems) using 500ng of input total RNA according to manufacturers protocol using 9 amplification cycles. The quality of the individual libraries was assessed using the High Sensitivity DNA Kit (Agilent). The individual libraries were quantitated via qPCR using the KAPA Library Quantification Kit, Universal (KAPA Biosystems) and equimolar pooled. The final pooled libraries were sequenced on an Illumina NextSeq 500 with paired-end 75 base read lengths.