GSM1366854: Biopsy-proven cancer; Homo sapiens; miRNA-Seq
Seminal fluid samples were collected into 20 ml Hanks’ Balanced Salt Solution (Gibco; Carlsbad, CA, USA) or phosphate buffered saline (PBS). All samples were processed within 2 h of collection. Briefly, mononuclear cells, including prostatic epithelial and urothelial cells, were separated from sperm cells using an isopycnic Percoll (GE Healthcare; Piscataway, NJ, USA) gradient. The mononuclear cell layer was removed and washed twice with PBS. Total RNA was isolated using TRIzol reagent (Invitrogen; Carlsbad, CA, USA) and cleaned up with RNeasy Mini kits (Qiagen, Valencia, CA, USA), including an on-column DNase digestion with the RNase-Free DNase Set (Qiagen), according to the manufacturer’s instructions. Library construction, using 1 µg of RNA and a TruSeq small RNA sample preparation kit (Illumina; San Diego, CA, USA), was carried out according to the manufacturer's instructions except that library cDNA was size-selected using a Pippin Prep  (Sage Science; Beverly, MA, USA) following the PCR amplification step.
GEO Accession
GSM1366854