GSM2633510: male rep 2; Drosophila melanogaster; Hi-C
~3000 embryos were collected in a saline buffer (0.03% Triton X-100, 0.4% NaCl) 16-18 hr after egg laying, then de-chorionated for 5 min in fresh bleach. Embryos were sorted with a Union Biometra COPAS Large Particle Sorter (Union Biometra Inc, Holliston, MA), and then processed for Hi-C as in Sexton et. al. 2012. Aliquots of 5 µg 3C DNA were sonicated in a Bioruptor (Diagenode) in 50 µl volumes in sonication buffer (50 mM Tris-HCl, pH 8, 10 mM EDTA, 1% SDS) to obtain a smear between 500-1500 bp. The sonicated 3C DNA was then purified by phenol/chloroform extraction and ethanol precipitation and quantified with the Qubit dsDNA assay (Invitrogen). Libraries for paired-end sequencing were made from 500 ng aliquots of sonicated 3C DNA using Illumina reagents and protocols, with size selection for products of ~800 bp.
GEO Accession
GSM2633510