GSM1854757: big_rep1; Saccharomyces cerevisiae; RNA-Seq
Total RNA was isolated by a standard hot phenol method and treated with RNase-free DNaseI using Turbo DNA-free kit (Ambion) Genome-wide quantification of gene expression and poly(A) site usage was performed using 3’T-fill approach (Wilkening et al 2013; PMID: 23295673). We used 500-3000 ng of total RNA as starting material.
GEO Accession
GSM1854757