GSM5731692: MDA-MB-231 shControl; Homo sapiens; ChIP-Seq
The cells were resuspended in a swelling buffer in the presence of the protease inhibitor cocktail (Sigma) and then pelleted and resuspended in the SDS lysis buffer. The lysates were transferred to a Covaris microTUBE and sonicated with an E220 Covaris Ultrasonicator. Chromatin fragmentation (~500 bps) was verified through agarose gel electrophoresis. DNA was extracted using Qiagen mini prep based on manufacturer instructions. DNA libraries were prepared for sequencing using standard ChIP Illumina protocols
GEO Accession
GSM5731692