WGBS of MOLM-13 cells: Aza treatment 8 days
MOLM-13 cells were treated wit 5-Azacytidine for 8 days. Cells were collected and lysed to collect DNA for Whole genome bisulfite sequencing. Fragmented DNA was used for library construction with the Ovation Ultra low Methylation sequencing kit (Nugen) andt the bisulfite conversion was done with the EpiTect Fast Bisulfite Kit (Qiagen). PCR cycles were optimized via qPCR.