GSM5034266: ATAC-Seq siLBR_3; Homo sapiens; ATAC-seq
Using 50,000 intact cells as input. Cells were washed and lysed to generate a crude nuclei preparation. Library for ATAC-seq was prepared according to a published protocol (Buenrostro et al., 2013). Tn5 transposase tagmentation simultaneously fragments the genome and tags the resulting DNA with Illumina sequencing adapters.  DNA fragments were PCR amplified and subsequently purified using Qiagen MinElute PCR Purification Kit (Qiagen, Maryland, USA). Final library quality and quantity were analyzed and measured by Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA) and Life Technologies Qubit 3.0 Fluorometer (Life Technologies, Carlsbad, CA), respectively. The final libraries were sequenced using 150 bp paired-end reads on Illumina HiSeq Sequencer (Illumnia Inc., San Diego, CA). 
GEO Accession
GSM5034266