GSM1365548: B-ALL, hyperdiploid; Homo sapiens; Bisulfite-Seq
AllPrep DNA/RNA Mini Kit (Qiagen) according to manufacturer's instructions We sheared the DNA by sonication to 50–500 bp with a Covaris E220, followed by selecting 150- to 300-bp fragments using AMPure XP beads (Agencourt Bioscience). Genomic DNA libraries were constructed using the TruSeq Sample Preparation kit (Illumina). Two rounds of sodium bisulfite-conversion of DNA using the EpiTexy Bisulfite kit (Qiagen) was performed to get a conversion efficiency of >99%.
GEO Accession
GSM1365548