WES and RNA-Seq of paired tumor/normal samples in late-stage melanoma: Sample WES_N_NEOPS_42_R1
Hybridization-based enrichment for exome (and transcriptome) preparation was carried out using target-specific biotinylated probes binding the desired fragments of the DNA (and RNA) library in solution.  The fragment-probe duplexes were then isolated using streptavidin-coated beads via magnetic pull-down, amplified by PCR, and purified with SPRI beads. Target-enriched libraries were quantified by qPCR and sequenced on Illumina instrumentation following the manufacturer's protocols.