GSM5224824: 2VO treatment rep2; Mus musculus; RNA-Seq
The hippocampus were cut into pieces and transferred it to a 1.5 mL microcentrifuge tube with 2 mg/mL isolation solution, containing pronase (Sigma, Cat# P6911-1G) and 50 μg/mL DNaseІ (Sigma, cat. no. D5025) in 1 mL Hibernate A (Invitrogen, cat. no. A1247501)/B27 (Invitrogen, cat. no. 17504) medium (HABG). The tissue and solution were mixed for 30 min at 37℃ in a horizontal shaker at 200 rpm. After incubation, the tissue was gently triturated by polished tips, and the single cells were released. The purified cells were obtained by density gradient centrifugation at 800 g for 15 min, resuspended in 1x PBS (calcium and magnesium-free) containing 1% BSA, and then centrifuged at 200 g for 2 min. Single cells were concentrated and resuspended in the desired medium. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM5224824