Metagenomic Analysis of Viral Communities in Water Habitats and Feces of Migratory Birds
Briefly, the purified PCR products were ultrasonicated to produce DNA fragments of about 180 bp, and then treated with Klenow and dATP to generate 3’-dA overhangs. After ligation of the fragments to Solexa adaptors, the DNAs were subjected to PCR with the adaptor primers to construct a genomic DNA library.