Human gut metagenome sequence
Sample DNA concentrations were determined by PicoGreen assay. DNA was sheared to ~400 bp using the Covaris S2 acoustic platform and libraries were constructed using the Nugen Ovation Ultralow kit. The products were visualized on an Agilent Tapestation 4200 and size-selected using BluePippin (Sage Biosciences). The final library pool was quantified with the Kapa Biosystems qPCR protocol and sequenced on the Illumina NextSeq500 in a 2 x 150 paired-end sequencing run using dedicated read indexing.