GSM5034542: CTRL Infected 14 DPI small RNA Rep 2; Anopheles stephensi; miRNA-Seq
Mosquitoes were anesthesized with triethylamine and RNA was extracted from each individual mosquito using mirVana RNA extraction kit (Life Technologies) applying the protocol for extraction of total RNA. The NEBNext Ultra II RNA library kit (New England Biolabs) was used for RNA-Seq library construction for total RNA. Small RNA libraries were created using the New England Biolabs small RNA library protocol.
GEO Accession
GSM5034542