Thorn (lateral) of Bougainvillea
The thorn stage corresponded to the shoot apical meristem (SAM) in the Arabidopsis that has not entered the reproductive growth.The tissues were washed with distilled water, and briefly air-dried in a clean environment.The sample mixtures were lysed with 1 mL TRIzol reagent.Total RNA was prepared according to the manufacturer's instructions.The RNA library was constructed by the Novogene Co. Ltd. (Beijing, China). Three micrograms RNA per sample was used as the input material for library preparation. Polyadenylated (poly(A)) RNA was purified from total RNA with poly T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5X). After reverse transcription, the fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA), and the cDNA fragments with length in 150~200bp were preferentially selected for PCR. At last, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system. RNA sequencing was performed in an Illumina HiSeq 4000 system (Illumina, San Diego, CA, USA).