GSM5224082: SupT1 2 ug/mL Dox + 10 uM TMP Rep2; Homo sapiens; RNA-Seq
Cellular RNA was harvested using the RNeasy Plus Mini Kit with QIAshredder homogenization columns (Qiagen). RNA-seq libraries were prepared using the Kapa mRNA HyperPrep RNA-seq library construction kit (Kapa/Roche), with 6 min fragmentation at 94 °C and 9 PCR cycles of final amplification and duplex barcoding. Libraries were quantified using the Fragment Analyzer and qPCR before being sequenced on an Illumina HiSeq 2000 using 40-bp single-end reads in High Output mode.
GEO Accession
GSM5224082