RADseq for Orobache foetida
DNA was extracted from fresh floral buds using innuPREP Plant DNA Kit (AnalytikJenaInd, Berlin, Germany) following the manufacturers instructions. RAD libraries were prepared as following:  enzymatic digestion was performed using BglII and EcoRI and ligation with Y-shaped adaptors followed by a PCR with KAPA HiFi HS ReadyMix (KAPA BIOSYSTEMS). Size selection for fragments of approximately 350 bp was carried out using the E-Gel size select (Life technologies, CA, United States). Single-end sequencing was conducted with a read length of 51bp with the Illumina HiSeq 2500 platform (Illumina, CA, United States).