GSM1579172: H3122_vitro_vehicle_rep1; Homo sapiens; RNA-Seq
For direct RNA isolation from cell lines or tissue, PrepEase RNA Extraction kit (USB) is used according to the manufacturer's instructions. For TRAP based RNA extraction, tumor tissues are dissected from mouse and immediately subjected for tissue homogenization. Lysates cleared by centrifugation are used for immunoprecipitation with GFP coated sepharose protein G beads. Ribosome associated RNA is recovered directly from washed beads with RNAqueous-Micro total RNA isolation kit (Ambion). RNA samples are used for Hi-Seq library construction with the TruSeq RNA Sample Prep Kit v2 (Illumina) following the manufacturer’s instructions. Samples are barcoded with TruSeq SBS Kit v3 (Illumina).
GEO Accession
GSM1579172