GSM2628088: RelA ChIP-seq rep.1; Homo sapiens; ChIP-Seq
GM12878 cells were cross-linked with 1% formaldehyde. Chromatin was sonicated to an average size of 500 bp. Biological replicates were obtained using cells grown on separate days. Antibodies against RelA, RelB, cRel, p50 and p52 were used to immunoprecipitate protein-DNA complexes. Captured protein-DNA complexes were eluted from protein A beads and reverse-crosslinked. Eluted DNA was purified using PCR purification columns (Qiagen). ChIP-seq libraries were prepared using NEBNext library preparation kits (New England Biolabs)
GEO Accession
GSM2628088