GSM4594449: colorectal adenocarcinoma cells - T72; Homo sapiens; OTHER
Hi-C samples were generated according to Rao et al., 2014. Briefly, 3 million cells were crosslinked in 1% formaldehyde for 10 minutes at room temperature. Chromatin was digested with MboI, restriction fragments were filled in with biotinylated nucleotides, and ligated. Crosslinks were reversed and ligated DNA was purified and sheared to a length of 300-500 bp. Biotinylated fragments were purified using streptavidin beads. Library construction was completed using end-repair, adaptor ligation, and high-fidelity PCR.
GEO Accession
GSM4594449