GSM4594482: nasal respiratory epithelium, patient 5; Homo sapiens; RNA-Seq
Biopsies were snap frozen in liquid nitrogen immediately upon collection. For RNA extraction, tissues were placed in RLT Buffer (Qiagen) with beta-mercaptoethanol and lysed with stainless steel balls (5mm diameter, Schieritz and Huenstein AG) using a homogenizer (Big Prep, MB Biomedicals). Total RNA was isolated using RNeasy Mini Kit (Qiagen) following the manufacturer protocol. The quality and quantity of total RNA were evaluated with a Bioanalyzer (Agilent). Stranded cDNA libraries with suitable adapters for multiplexing were generated with Truseq RNA and DNA sample preparation kits (Illumina) following ribodepletion of the total RNA (200ng of total RNA per sample). Samples were multiplexed for sequencing in a HiSeq 2500 Sequencing system, generating 100bp single-end reads.
GEO Accession
GSM4594482