WGS MiSeq of Serratia sp. HRI
Genomic DNA was sent to the UFS Next Generation Sequencing Unit for library preparation and whole genome sequencing on an Illumina MiSeq platform. Library preparation process started with a quantification of DNA using a Quibit 3.0 Fluorimeter, thereafter an index PCR was done to attach dual indices and Illumina sequencing adapters using the Nextera XT index kit as per manufacturers instructions. Following this, a PCR clean up was done using using AMPure XP magnetic beads. Library concentrations were equalised, pooled and denatured and transferred to a V3 sequencing cartridge. The MiSeq run was started and allowed to proceed for 600 cycles (150bp x 2 paired end).