RNA-Seq of homo sapiens: adult tongue
Using Isolate II RNA Mini Kit (Bioloine), total RNA was isolated from irradiated spheres at half an hour, 2 hours and 24 hours post 2 gray of ionizing radiation. RNA was also isolated from two control samples one as the control for the first two time points, half an hour and 2 hours and the other one as the control for the last time point, 24hours post-irradiation. The whole mRNA content of each sample was converted to cDNA using TruSeq RNA Library Prep Kit