fall14_Sarracenia_rubra_wherryi_4
Collections of pitcher plant fluid were made from an outdoor common garden from 15 species across a two year period sampling both in the spring and fall. DNA extractions followed the Lodhi et al. (1994) protocol with two separate chloroform:iso-Amyl alcohol cleanings. Each sample was treated with RNAase A. The V4 hypervariable region was targeted using the 515F and 806R primers developed by Caporaso et al. (2012).