MNase-Seq of saccharomyces cerevisiae: TBP-FRB H2A nucleosomes without rapamycin: Sample TBP-FRB (no RAP) AA nucleosomes biological rep 1 technical rep 3
The Anchor Away strain TBP-FRB was grown to log phase and fixed with paraformaldehyde. Mononucleosomes were released by MNase digestion and the nucleosome-associated DNA fragments were mapped by deep sequencing.