GSM1577175: WT3; Mus musculus; RNA-Seq
Total RNA from mouse ES cells was isolated with TRIzol Reagent (Life Technologies, 15596) following the manufacturer’s instructions. RNA was treated with DNase I (NEB, M0303S) to remove contaminated genomic DNA. After that, RNA was depleted of ribosomal RNA with the Ribo-Zero kit (Epicentre). The ribo-depleted RNA samples were amplified with the TruSeq RNA Sample Prep Kit (Illumina) for Solexa sequencing.
GEO Accession
GSM1577175