GSM1581175: NOD naïve; Mus musculus; RNA-Seq
Briefly, 10ug of total RNA was polyA selected twice using Oligo(dT)25 beads (Life Technologies) and NEB oligo(dT) binding buffer. PolyA-selected RNA was fragmented, repaired and cleaned using Zymo RNA concentrator-5 kit.  30ng of polyA-selected RNA per sample were used to make RNA-seq libraries. Adapter was ligated to RNA, RNA was reverse transcribed and second adapter was ligated on cDNA.
GEO Accession
GSM1581175