NextSeq 500 sequencing; RNA-sequencing of prostate cancer xenografts, their 3D cultures and prostate cancer cell-lines.
RNA-sequencing of prostate cancer xenografts, their 3D cultures and prostate cancer cell-lines.
PDX tumor tissue was cut into small pieces (1–0.5 mm) with a scalpel blade and then digested in CollagenaseType I media solution (200U/ml Cat#SCR103, Millipore) at 37 °C for 45-60 min. RNA was extracted using the Rnasy Mini Kit (Qiagen) and sample quality was assessed using Agilent Bioanalyzer. RNA libraries were prepared for sequencing using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®
Experimental Factor: growth condition
2D
Experimental Factor: cell line
LNCaP