GSM5752959: NSSCC-IgG-IP miR-34c Rep3 [miR_34c_IgG3]; Homo sapiens; RIP-Seq
The cell lysates were centrifuged at 16,000g for 10 minutes at 4°C and incubated for 180 minutes with Protein G Sepharose beads (Thermo Fisher Scientific) coupled with anti-Ago antibody (10 μg, Invitrogen) or rat IgG isotype control (10 µg, Millipore). cDNA libraries were generated using the SMARTer Stranded Total RNA Sample Prep Kit (Takara Clontech)