GSM1370138: Female WT stage D; Vitis vinifera subsp. sylvestris; RNA-Seq
The frozen buds were ground into a fine powder in liquid nitrogen and total RNA was extracted and purified with the SpectrumTMTotal RNA kit (Sigma-Aldrich, Inc, Spain) and Qiagen RNAeasy Mini Spin Columns (Qiagen, Valencia, CA, USA), respectively,according to the manufacturers'instructions. The concentration and purity of total RNA were determined using a Synergy HT Nanodrop system (Biotek, Germany), with the software Gen5TM (Biotek, Germany). The quality of the RNA was confirmed on a denaturing gel electrophoresis. For each sample, 25µg of total RNA were utilized for transcriptome sequencing RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM1370138