GSM4404030: HSCEpigenomics_SP3KO_scRNA; Mus musculus; RNA-Seq
E10.5 embryos were collected from Sp3+/- females mated to Sp3+/- males. Dorsal aorta regions were isolated and dissociated using 0.125% collagenase (Sigma) for 40 min. Cells were then washed and stained with the following antibodies (1:200): Ter119, ESAM, CD41, CD144, CD31, and DAPI (Invitrogen, D3571) for viability. The cells were pooled by genotype and sorted for endothelial cells on a BD Influx into 1xPBS + 10% FBS in 1.5 LowBind Tubes. Libraries were prepared using 10x Genomics protocol (Chromium Single Cell 3' Reagent Kits v3, CG000183 RevA).
GEO Accession
GSM4404030