GSM5082087: i14G1-10_Ribo-seq_1 [OligodT cleaned RNA]; Homo sapiens; RNA-Seq
Cell lysates were thawed and messanger RNA was captured with 15ul of NEB oligo(dT) beads, and washed according to manufacture guidelines. Purified messanger RNA was eluted at 70C with 60ul of 10mM Tris-Cl pH 7.5 and stored at -80C.For library preparation RNA was used for the bulk MARS-seq  protocol (Jaitin et al. 2014 Science.343:776-9). MARS-seq libraries were sequenced using Illumina NextSeq 500 High Output v2 Kit (75 cycles).
GEO Accession
GSM5082087