GSM5775747: Input_FOXA2; Homo sapiens; ChIP-Seq
Crosslinking was carried out using 1% formaldehyde at room temperature. Formaldehyde was quenched with 1.25M glycine. Cells were washed with PBS, lysed, sonicated and the chromatin was used in a Chromatin Immunoprecipitation (ChIP) reaction (HighCell# ChIP kit protein G (Diagenode Inc., NJ) per the manufacturer's recommendation using the Histone H3 acetyl Lys27 antibody (Active Motif, CA). The ChIP DNA was reverse crosslinked, eluted and purified, and used to prepare next generation sequencing libraries (KAPA Hyper Prep, KAPA Biosystems, MA). The ChIP sequencing (ChIP-Seq) libraries, after appropriate size selection using AMPure XP Beads (Beckman Coulter Sciences, IN) were sequenced with a read-length of 75bp at the UTSW McDermott Sequencing Core.