TCR-seq of mus  musculus:  adult femal kideny CD8+CD62L+CD44+ T cells
TCR repertoire library was prepared by using multiple specific primers for V gene segments of TCR and a universal reverse primer specific to the constant region of TCR.  In the rst round of amplication,  cDNA was mixed with 23 ILMN_TRBV mixed primers, 1 specic ILMN_TRBC primer as wellas the 1e7 copies of barcoded plasmid template mixture. A second round PCR was done to add the specific P5 and P279 llumina index primers.The purified product was sequenced on an Illumina HiSeq Xten instrument.