GSM1366300: Sperm1A; Gallus gallus; Bisulfite-Seq
DNA was extracted from 100 µl of chicken sperm, directly frozen upon sampling. Thawed sperm cells were washed twice in water and 200 µl of Laird’s lysis buffer (Tris-Col 100mM, EDTA 5 mM, NaCal 20 mM, SDS 2%, DTT 40 mM and proteinase K 500 µg/ml) was then added and was incubated overnight at 50°C. The sample was then purified by two rounds of phenol-chloroform/chloroform extraction followed by ethanol precipitation. Standard Illumina paired-end protocol
GEO Accession
GSM1366300