metagenomic analysis of activated sludge of textile and dyeing wastewater treatment plant
Total genomic DNA of FSA and FSO was extracted in duplicate using the FastDNA? Spin kit for soil (MP Biomedicals, USA) following the manufacturerĄŻs protocol. The concentration and purity of extracted DNA were determined by NanoDrop microspectrophotometry (ND-2000, Thermo Fisher Scientific, US). The V3-V4 region of bacterial 16S ribosomal DNA was amplified by polymerase chain reaction (PCR) using the barcoded primer pairs of 338F/806R. Purified PCR amplicons were sequenced by Illumina MiSeq platform with 2 ¨w 250 bp paired-end reads in Shanghai Majorbio company