GSM4815961: RNA-seq-HBEC-SARS2-lowMOI-6hpi; Homo sapiens; RNA-Seq
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Infected cells were lysed and two aliquots were processed in parallel for ribo-seq and RNA-seq. For RNA-seq, total RNA was isolated using ZymoClean RNA-Clean and Concentrator kit and subjected to Illumina stranded mRNA prep. Ribo-seq libraries were prepared in house following published protocols. Both libraries were sequenced using Illumina NextSeq following the manufacturer's protocols.
GEO Accession
GSM4815961