GSM5171223: SS5_1_1; Homo sapiens; RNA-Seq
Total RNA was extracted using Trizol reagent. Total RNA was purified by Oligo(dT)-attached magnetic beads. Purified RNA was fragmented into small pieces, and the first-strand cDNA was generated using random priming, followed with second-strand cDNA synthesis. After end repairing, the cDNA was amplified by PCR and the products were validated on the Agilent Technologies. The final library was generated after heated denature and circularization of the PCR products. Then the single end 50 bases reads were generated on BGIseq500 platform (BGI-Shenzhen, China).
GEO Accession
GSM5171223