RNA-seq of Triticum aestivum under P starvation; replicate 2
Total RNA was extracted from the treated root samples along with the control by Trizol (Invitrogen) as per the manufacturers instruction. The quality and quantity were checked on the 1% denaturing RNA agarose gel and Nanodrop respectively. Subsequently, all the RNA used for library preparations were checked for their RNA integrity number 8.5 using Bioanalyzer (Agilent, USA). The quality control (QC) passed RNA samples were then processed for library preparation. The Paired-ended (PE) libraries were prepared from the total RNA using illumina TruSeq stranded mRNA library prep kit as per the instructions (Illumina Inc., USA).