GSM3301722: R7-D13-16hAug23-9SR-T1-33N: Donor 2, ST2-transduced Treg + TCR + IL-33 (16h); Homo sapiens; RNA-Seq
Activated cells were pelleted (500g for 5 min), lysed and total RNA isolated with RNAzol RT per manufacturer's instructions. All samples used in this study had an RNA integrity number of 9.8-10. mRNA enrichment and library preparation with NEBNext Poly(A) mRNA Magnetic Isolation Module, NEBNExt Ultra II Directional RNA Library Prep Kit for Illumina.
GEO Accession
GSM3301722