Deep-sea sediments and coral flocculant material impacted by the Deepwater Horizon (DWH) oil spill
9 samples (3 replicates from each sample type; sediment MC 294 4664 0-2cm, sediment MC 294 4662 0-2cm and coral flocculant material (from corals A5 and B8)  The 16S rRNA gene V4 variable region was amplified with primers 515F and 806R (Caporaso et al., 2012), designed to target both 16S rRNA bacterial and archaeal sequences, with barcodes on the forward primer.  Pooled and purified PCR products were prepared into a DNA library following Illumina TruSeq DNA library preparation protocol. Sequencing was performed at MR DNA (www.mrdnalab.com, Shallowater, TX, USA) on a MiSeq following the manufacturer’s guidelines