GSM5033046: Tumor-infiltrating empty vector-overexpressing Pmel CD8 T cells, 4; Mus musculus; RNA-Seq
At day 7 post-adoptive transfer, immune cells were isolated from the tumors. For each biological replicate, 2000 positively transduced (Thy1.1+) live (7AAD-) Pmel T cells from each mouse were directly sorted into lysis buffer containing Protector RNase Inhibitor. RNA-seq libraries were prepared using a modified SMART-Seq2 protocol and sequenced with a NextSeq 500 sequencer using a high output V2 75 cycle kit (Illumina) in a 37 bp paired-end mode.
GEO Accession
GSM5033046