GSM2633756: DDX1_CLIP_2; Rattus norvegicus; RIP-Seq
INS-1 cells were irradiated with UV light (254 nm) to induce covalent RNA-protein cross-linking.Then the DDX1 and cross-linked RNAs were immunoprecipitated with anti-DDX1 antibody (Proteintech, 11357-1-AP), and digested with Mnase, labeled with 32P at 5’ terminal. The DDX1/RNA complex was excised from the SDS/PAGE gel visualization with a phosphorImager (fig). The cross-linked RNAs were recovered from the DDX1/RNA complex The cross-linked RNAs were recovered from the DDX1/RNA complex, ligated to RNA adaptor, converted to cDNA, and sequenced on the Hiseq 2500 illumina platform.
GEO Accession
GSM2633756