GSM1364750: RNAseq_single_egg_replicate2; Xenopus laevis; RNA-Seq
Total RNA was isolated using TRizol® Reagent (Ambion) and RNeasy Kit (Quiagen), and rRNA depleted with Ribo-Zero™ rRNA Removal Kits (Epicentre). rRNA-depleted RNA was then heat-fragmented in 40mMTris-acetate, 100mM potassium acetate and 30mM magnesium acetate (pH8.2) and used for cDNA synthesis. First cDNA strand was obtained from random examers primers using Superscript III (Invitrogen); second strand was made with E.coli DNA polymerase I (NEB), E.coli DNA ligase (NEB) and T4 DNA polymerase (Promega). Purified cDNA was used for strand-specific Illumina sample preparation. Libraries were constructed according to standard Illumina protocols. Briefly cDNA was ligated with adapter sequences, size selected (300bp), and amplified by PCR.
GEO Accession
GSM1364750