AS24hC2
Eighteen 6- or 7-week-old Beagle puppies were purchased from the National Canine Laboratory Animal Resource Center (Guangzhou, China). The pre-experimental detection and rearing environment were the same as our previous description[25,28]. Puppies were equally divided into three groups according to the time point of larvae migrate in the host: 24 hours post infection (hpi) group, 96 hpi group and 36 days post-infection (dpi) group, and three experimental dogs and three control dogs were included in each group Puppies in the experimental group were infected orally with 300 infected eggs to simulate natural infection, while puppies in the control group were instilled with the same dose of saline. Puppies were general anesthetized with Zoletil 50 (Virbac, France) and injected with KCI solution into their hearts to achieve sudden humanitarian death. Puppy spleens were then collected, the surface was rinsed with saline, and 2 g of spleen tissue was excised and stored in liquid nitrogen at -84 C for extraction of total tissue RNA. Potential laboratory waste and infectious clinicals, such as infected eggs and remaining puppy tissue, were sterilized by autoclaving prior to processing.