Cell Cycle Reprogramming for PI3K Inhibition Overrides Relapse-Specific C481S BTK Mutation Revealed by Longitudinal Functional Genomics in Mantle Cell Lymphoma
DNA was isolated from purified MCL cells, and a sequencing library was created with the Illumina TruSeq (v3) DNA Preparation kits (FC-121-1031). Following isothermal cluster generation (PE-401-3001) and 75x75 paired-end (PE) sequencing on the HiSeq2500 (FC-401-3001), the samples underwent primary analysis with the Illumina base calling and primary analysis software (HCS 1.4, CASAVA 1.8.2, and RTA 1.2).