16S rRNA amplicon from water at shrimp pond one
Total environment DNA was extracted from 1 L of water samples using DNeasy PowerSoil Isolation Kit (QIAGEN Cat. No: 47014). The quality and quantity of DNA were checked on agarose gel (8% w/v) and through NanoDrop ND-1000 spectrophotometer. The 16S rRNA gene were paired-end sequenced through Illumina HiSeq 2502 platform to investigate the composition of the microbial community by BGI (Beijing Genomics Institute, Shenzhen, China).