GSM4404138: Male 2; Mus musculus; RNA-Seq
Suspensions of dissociated cells were washed and suspended in PBS containing 0.04% BSA, and each suspension was processed as follows.  Cells were counted on Countess II automated cell counter (ThermoFisher), and 12,000 cells were loaded for capture onto an individual lane of a Chromium Controller (10X Genomics). Single cell capture, barcoding and library preparation were performed using the 10X Chromium platform according to the manufacturer's protocol (#CG00052) using version 2 (V2) chemistry for the first set of male and female samples and version 3 (V3) chemistry for the second set. cDNA and libraries were checked for quality on Agilent 4200 Tapestation, quantified by KAPA qPCR.
GEO Accession
GSM4404138