Illumina HiSeq 2500 sequencing; Identification and characterization of developmental, tissue, and evolutionary transcription start sites in Bos taurus indicus
CAGEseq in bovine Ovary
ftp://ftp.faang.ebi.ac.uk/ftp/protocols/assays/ROSLIN_SOP_CAGE-library-preparation_20190903.pdf
project
FAANG
assay type
CAGE-seq
sample storage
frozen, -70 freezer
sample storage processing
cryopreservation in liquid nitrogen (dead tissue)
sampling to preparation interval
3.0
extraction protocol
https://www.thermofisher.com/document-connect/document-connect.html?url=https%3A%2F%2Fassets.thermofisher.com%2FTFS-Assets%2FLSG%2Fmanuals%2Fcms_055423.pdf&title=bWlyVmFuYSZ0cmFkZTsgbWlSTkEgSXNvbGF0aW9uIEtpdCAoRW5nbGlzaCAp
library preparation location
Western General Hospital, Edinburgh
sequencing location
Western General Hospital, Edinburgh
experiment target
TSS
cage protocol
ftp://ftp.faang.ebi.ac.uk/ftp/protocols/assays/ROSLIN_SOP_CAGE-library-preparation_20190903.pdf
sequencing primer provider
not provided
sequencing primer catalog
not provided
sequencing primer lot
not provided
restriction enzyme target sequence
CAGCAG
rna integrity number
7.3