RNA-seq of Ribo-Zero and Rnase R treated total RNA from leaves of Arabidopsis thaliana
One hundred nanogram aliquots of R- or R+ RNA samples were used to prepare indexed libraries using the TruSeq RNA Sample Preparation Kit (Illumina) in accordance with the manufacturers instructions with minor changes. The protocol was modified by omitting the mRNA purification step and reducing reaction volumes by one-third at subsequent steps.