Mitochondrial DNAs of inbred MSC12
Plants were grown from seed for 45 days and 50 g of green leaf tissue was used for isolation of mitochondria by differential centrifugation through Percoll-density gradients as described in the 2014 PhD thesis of Angel R. Del Valle-Echevarria (University of Wisconsin-Madison).  Mitochondrial DNA was extracted as described Klein et al. (1994; Plant Journal 6:447-455) and sequenced at the University of Wisconsin Biotech Center using the Roche 454 FLX platform and protocols as recommended by the manufacturer.