RNA-Seq of Fraxinus hupehensis:The seeed of not treatmentFirst biological repeat
After the total RNA samples were qualified, the mRNA was enriched with the magnetic beads with Oligo (dT), and the fragments were added to the obtained mRNA to make the fragments a short fragment, and then the mRNA as a template after the fragment was used as a template, and the first chain of cDNA was synthesized with six base random primers, and the buffer solution, dNTPs, RNaseH and DNA Polymerase I continued to synthesize the cDNA. The two strand was purified by QIAQuick PCR kit and eluted with EB buffer. After the elution and purification of the purified double strand cDNA, the end repair, the base A, and the sequenced joint were treated, and then the size fragment was recovered by agarose gel electrophoresis and the PCR amplification was carried out to complete the whole library preparation.