miRNA-Seq of Homo sapiens: colorectal cancer adjacent normal from patient014
miRNA profiles of CRC  tumor and adjacent normal tissues from the same patients were generated by deep sequencing, using Illumina Nextseq500. Total RNA was isolated and purified from tissues by using the TRIzol reagent (Invitrogen). The quality and concentrations of the RNA were respectively measured with Agilent 2100 BioanalyzerTM (Agilent) and Nanodrop ND-2000 spectrophotometer (Thermo Scientific). Samples with OD260/280 ratio of > 1.8 and RIN values of > 8.5 were further selected for miRNA library construction. Library construction for miRNA sequencing was done using SureSelect Strand-Specific miRNA Library Preparation for Illumina Platform Sequencing (Agilent Technologies) according to the manufacturer's protocol.