GSM4944007: D7_Smart-seq2_F7_exp2; Mus musculus; RNA-Seq
The Smart-seq2 protocol (Picelli et al., 2014) was used to generate amplified cDNA libraries from EYFP+RFP+ and EYFP+RFP- cells isolated from ligated left common carotid arteries of VSMC lineage-labeled animals (Myh11-EYFP-Ki67/RFP) 7 days after surgery and EYFP+ cells from unligated litter mate control animals in 2 separate experiments (experiment 1: 4 ligated arteries and 1 control arteries; experiment 2: 5 ligated arteries and 2 control arteries). Cells were processed as described (Dobnikar et al., 2018) with addition of ERCC spike controls (diluted 1:80,000,000, Invitrogen). Sequencing libraries were generated using the Nextera XT kit and pooled libraries were sequenced using a paired end protocol (HiSeq 2500, Illumina).
GEO Accession
GSM4944007