GSM4721866: Day 12-control-rep3; Homo sapiens; RNA-Seq
Total cellular RNA was isolated at respective time points using RNeasy mini kit (Qiagen, Carlsbad, CA) according to the manufacturer's protocol. RNA quality was checked at the TapeStation using an RNA ScreenTape. 500 ng of total RNA were processed using the KAPA HyperPrep mRNA kit (Roche, Pleasanton, CA) according to the manufacturer's instructions. Briefly, mRNAs were captured with oligo-dT magnetic beads and fragmented for 6 min at 94°C. First strand synthesis was performed on fragmented RNA, followed by second strand synthesis and dA-tailing. Adapter ligation was performed using KAPA single-indexed adapters (KAPA, Pleasanton, CA). The adapter-ligated fragments were amplified with 10 PCR cycles. Final libraries were then pooled and sequenced on a HiSeq4000 single-end 50 nt.
GEO Accession
GSM4721866