ki1 developing ear siRNAs, plant 1
Small RNA-enriched RNA was extracted from 50 to 100 mg developing ear tissue (4-6 cm stage) using the mirVana miRNA Isolation Kit (Life Technologies; AM1560) with Plant RNA Isolation Aid (Life Technologies; AM9690). Libraries were prepared using a NEXTflex Small RNA Library Prep Kit v3 for Illumina (Bioo Scientific; 5132-05) with 13 cycles of PCR amplification and the gel-free size selection and cleanup option.