GSM4404082: HCT116_A2_2_WT_eCLIP; Homo sapiens; RNA-Seq
For RNA_seq samples, RNA was harvested using Trizol reagent. Illumina Whole Transcriptome RNA Sample Prep Kit (strand specific) was used with 50 ng-1 ug of total RNA for the construction of library. For the eCLIP-seq, cell cytoplasmic fraction was obtained wtih hypotonic buffer from 10 million cell and used for immunprecipitation or size-matched input samples. RNA libraries were prepared for sequencing using standard Illumina protocols or eCLIP-seq protocol (Van Nostrand, et. al, Nature Methods, 2016, 13, 508-514)
GEO Accession
GSM4404082