Calophya_latiforceps_Salvador_repA
Psyllid DNA was extracted from 20 adults using the DNeasy Blood & Tissue Kit (Qiagen, Venlo Netherlands). Sequencing was performed at the RTSF Genomics Core at Michigan State University. Genomic DNA was used as input for PCR amplification of microbial 16S rRNA genes using indexed primers as described previously (Caporaso et al. 2012). After normalizing, PCR products were pooled and cleaned. Pooled products were loaded on a standard MiSeq flow cell (v2) and sequenced in a paired end 2x250bp format.