GSM1576759: HCV110 mRNA replicate2; Homo sapiens; RIP-Seq
UV crosslinked cells were lysed in 1x PXL buffer, lystates were Dnase treated, partially RnaseA digested, clarified by centrifugation, and Argonaute-mRNA complexes isolated with pan-Ago 2A8 antibody. Libraries were prepared as in Moore M et al. 2014 but with the following modifications. The 2nd PCR step was used to introduce library specific indices that were additionally compatible with MiSeq sequencers using version2 chemistry.
GEO Accession
GSM1576759