RNA-Seq of LPS stimulated isolated mus musculus monocytes
RNA was isolated from bone marrow monocytes isolated by negative selection using magnetic-activated cell sorting (MACS, Miltenyi Biotec) according to manufacturers instructions. RNA was used to generate barcoded cDNA libraries using the NEBNext Ultra RNA library prep with rRNA depletion. Indexed libraries were sequenced in the 2x150bp configuration on the Illumina HiSEQ platform.