MG of Simocephalus sibiricus
Genomic DNA samples with qualified quality were randomly broken into fragments with a main peak of about 250~300bp by ultrasonic high performance sample processing system (COVIS).Then the DNA fragments were purified, the ends were repaired, the "A" base was added to the 3 'end, and the library joints were added to both ends.In order to obtain sufficient quantities, the DNA template with the connector was amplified and enriched using PCR.The concentration of DNA library was quantified by Agilent Bioanalyser instrument and real-time PCR method. After qualified quality control, it could be sequenced by computer.We used Illumina's Novaseq family of platforms to perform high-throughput sequencing on each qualified library.The raw image data obtained by sequencing was converted into raw reads (double-end-reads) by Illumina Base Calling software. The data was stored in FASTQ file format and called raw data.