GSM5345943: IgAN_sample42; Homo sapiens; RNA-Seq
Biopsy cores from kidney disease patients were obtained from punch biopsies performed between 2010 and 2017 and stored in RNAlater (Qiagen, Hamburg, Germany) at -80°C. We collected samples from 46 patients with IgA nephropathy, 3 with diabetes mellitus nephropathy, 3 with focal segmental glomerulosclerosis, 3 with lupus nephritis, 4 with membranous nephropathy, and 9 with minimal change disease, according to sample availability. Patients with profound kidney dysfunction and those with an estimated glomerular filtration rate (eGFR) < 30 mL/min/1.73 m2 were not considered for this study. To establish the control group, normal cortical tissues were collected from the non-cancer-affected cortex of 22 renal carcinoma patients who exhibited no evidence of CKD (eGFR < 60 mL/min/1.73 m2 or detected dipstick albuminuria). These nephrectomy control group samples were also stored in RNAlater immediately after surgical excision via the same protocol used for the biopsy core tissues. The frozen cortical tissues from the nephrectomy controls were initially cut into the size of a biopsy core, and all collected kidney tissues were manually microdissected under a stereomicroscope (Olympus, Japan) to remove glomeruli. After thorough removal of glomeruli, total RNA was extracted from the remaining tubulointerstitial tissues using an RNeasy Micro Kit (Qiagen, Hamburg, Germany). A TruSeq Stranded Total RNA Library Prep Kit (Illumina, CA, USA) was used to construct cDNA libraries. Paired-end RNA-seq was performed on the HiSeq 2500 platform (Illumina, CA, USA), and 100-base-pair sequences were obtained as in FASTQ format.
GEO Accession
GSM5345943