H3K9ac ChIP-Seq analysis of foreskin keratinocyte from skin01 (A02884)
H3K9ac ChIP-Seq analysis of foreskin keratinocyte from skin01 (A02884) using Illumina Genome Analyzer IIx
Refer to document 'REMC ChIP and Library Construction Protocol' from the Farnham Lab at the Roadmap Epigenomics Project site, Experimental Protocols page (URL: http://www.roadmapepigenomics.org/protocols/type/experimental/)
EXPERIMENT_TYPE
Histone H3K9ac
EXTRACTION_PROTOCOL
Refer to document 'REMC ChIP and Library Construction Protocol' from the Farnham Lab at the Roadmap Epigenomics Project site, Experimental Protocols page (URL: http://www.roadmapepigenomics.org/protocols/type/experimental/)
EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR
Bioruptor
EXTRACTION_PROTOCOL_SONICATION_CYCLES
20 min
CHIP_PROTOCOL
Refer to document 'REMC ChIP and Library Construction Protocol' from the Farnham Lab at the Roadmap Epigenomics Project site, Experimental Protocols page (URL: http://www.roadmapepigenomics.org/protocols/type/experimental/)
CHIP_PROTOCOL_CHROMATIN_AMOUNT
5 ug
SIZE_FRACTION
128-501 bp
CHIP_PROTOCOL_BEAD_TYPE
Protein A/G Beads
CHIP_PROTOCOL_BEAD_AMOUNT
30 ul
CHIP_PROTOCOL_ANTIBODY_AMOUNT
10 ul
CHIP_ANTIBODY
H3K9ac
CHIP_ANTIBODY_PROVIDER
Cell Signaling Technology
CHIP_ANTIBODY_CATALOG
9649
CHIP_ANTIBODY_LOT
1