Fragment of nirS from Accumulibacter UW-LDO-IC
PCR amplification of UW-LDO-IC gene fragments was carried out on extracted genomic DNA from the lab-scale SBR, in a 25 L reaction volume with 400 nM of each forward and reverse primer. The PCR program consisted of an initial 10-min denaturation step at 95C, followed by 30 cycles of 95C for 30 s, 64C for 30 s, and 72C for 30 s, and then a final extension at 72C for 5 min.