Whole genome sequencing of Acropora hyacinthus polyps
DNA was extracted from sperm pellets and polyp tissue using  Macherey-Nagel NucleoSpin Tissue Mini kit columns and protocol. Nextera full genome libraries were generated using a modified, low-volume protocol optimized for coral DNA (Lpez-Nandam et al.). We constructed two replicate libraries for each DNA extraction (Fig. 1b). Libraries were sequenced first on an iSeq 100 for quality control and then on a NovaSeq 6000 S4 at the Chan-Zuckerberg Biohub Sequencing facility in San Francisco, CA, USA.