NextSeq 500 sequencing
Nascent RNA from flash-frozen cells was isolated as described by Hirabayashi et al., (2019)
CAGE-based libraries were generated according to the no-amplification non-tagging CAGE libraries for Illumina next-generation sequencers (nAnT-iCAGE) protocol. All CAGE-based libraries were sequenced in single-read mode on an Illumina NextSeq500 platform.