RNA-seq of Triticum aestivum spikes 48 hours after mock (H20) inoculation
The sample was collected 48 hours after Mock (H20) treatment and consisted of pools of six individual heads. Heads were immediately shock-frozen in liquid nitrogen and stored at -80C. RNA of pooled samples (100 mg) was extracted from 100 mg of ground tissue using the RNeasy plant mini kit (Qiagen, Venlo, The Netherlands) and checked on a Bio-Rad Experion automated electrophoresis unit (Hercules, CA). Illumina HiSeq 2500 strand-specific RNA-seq libraries were sequenced in the 125 bp paired-end mode by GATC Biotech (Konstanz, Germany- now part of Eurofins Scientific).