GSM4816645: K0008-E4-d; Mus musculus; RNA-Seq
Mice were anesthetized with Avertin (250 mg/kg of body weight, i.p.) and perfused transcardially with cold 0.1 M PBS, pH 7.4: left hemisphere fixed in 4% paraformaldehyde for histology. The cortex was dissected from the other and snap frozen on dry ice for RNA and protein isolation RNA was isolated from cortex and purified using RNeasy mini kit (Qiagen) per the manufacturer's protocol. RNA quality was assessed using 2100 Bioanalyzer (Agilent Technologies) and samples with RIN > 8 were further used for sequencing. Sequencing libraries were generated by Novogene. scRNA libraries were generated with Chromium single cell 3' chip kit v3 for scRNA-seq (10x Genomics) according to the manufacturer's instructions and then the quality assessed using Agilent Bioanalyzer 2100. Sequencing was performed using NovaSeq (Illumina) by MedGenome Inc. with a custom sequencing setting (28bp for Read1 and 91bp for Read2) to obtain an average sequencing depth of 45K reads/cell.
GEO Accession
GSM4816645