Geobacillus thermoleovorans ARTRW1
To create sequencing libraries, 100 ng genomic DNA and TruSeq Nano DNA Low Throughput Library Prep Kit (Catalog No: 20015964; Illumina) were used. In terms of size distribution and quantity of libraries created, quality control was carried out using 2100 Bioanalyzer (Agilent Technologies, USA). For normalization, the DNA library was diluted to 10 nM using dilution buffer (Tris-HCl 10 mM, pH 8.5, 0.1% Tween 20). Synthesis sequencing (SBS) was performed using HiSeq Rapid SBS Kit v2 (Catalog No: FC-402-4023; Illumina) as single fragment readings (2 x 150 bp; PE) obtained by combining two fragments end-to-end. The cBot 2 system (Illumina) was used for bridge amplification and the Illumina HiSeq 2500 platform was used for sequencing.