WGS of Arabidopsis thaliana: pooled DNA from F2 cross
From each individual the apex of the main stem was collected onto dry ice shortly after bolting and stored at -80C. Apices from the bulk were pooled and ground together in liquid nitrogen. DNA was extracted using a CTAB method. Genomic DNA library preparation was carried out by Novogene (UK) Company Limited using NEB Next Ultra DNA Library Prep Kit (Cat No. E7370L). The libraries were sequenced on a Illumina NovaSeq 6000 machine with 300 cycles (150bp paired-end reads).