16S V1-V2 Amplicin sequence of gut mrcrobiome: blood loss of rabbits
We used NewZealand rabbits to create a blood loss complication and designed a five-time-point fecal sampling strategy including 24-hour pre-blood loss treatment, 24-hour, 36-hour, 48-hour and 1-week post-blood loss treatment. Gut microbiome composition and diversity were analyzed using 16S rRNA V1-V2 gene sequencing and downstream -diversity, -diversity, and taxonomy analysis.