RNA-Seq of rice: 2DAP panicle
A total amount of 1.5g RNA was used for the RNA sample preparations. Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA). mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. After 3' and 5' adapter ligation and cDNA synthesis, the library preparations were sequenced on an Illumina Hiseq 4000 platform and 150bp paired-end reads were generated.