RNA-seq of Cryptops iheringi: seven venom glands from adults specimen
Seven Cryptops iheringi specimens were collected in the city of So Paulo, Brazil. The heads of the specimens were submitted to dissection of venom glands for transcriptomics. Total RNA was extracted using TRIzol reagent. mRNA was separated with magnetic beads with oligo (dT) using Dynabeads mRNA DIRECT kit Quant-iT RiboGreen RNA reagent and Kit. mRNA integrity was evaluated in a 2100 Bioanalyzer, picochip series (Agilent Technologies). A cDNA library was generated following the standard TruSeq RNA Sample Prep Kit protocol (Illumina, San Diego, CA). ABI StepOnePlus Real-Time PCR System was used for library sample quantification before sequencing. The cDNA library was sequenced on Illumina HiSeq 1500 System, in a Rapid Run mode in a 2-lane paired-end flow cell, run for 300 cycles, generating 250 bp paired-end reads for each fragment, according to the standard manufacturer's protocol (Illumina). After, Bioinformatics analysis was performed. The software assembler used  was rnaSPAdes (55 K-mer).