GSM2633805: 4G8_RIP_2; Homo sapiens; RIP-Seq
Total RNA and genomic DNA isolated from Jurkat T cells using Trizol according to the manufacturer’s instructions (Thermo Fisher Scientific) Library preparation were prepared from RNA using the NEBNext Directional Ultra RNA kit and Indexing primers (E7530, and E7335, respectively, both NEB) according to the manufacturer’s instructions, except that no initial RNA fragmentation were performed. Libraries were pooled and pair-end sequenced (2x75 bp) on the MiSeq platform (Illumina). Sequencing libraries for DNA were prepared using the NEBNext DNA ultra kit (E7370, NEB), using NEBNext Indexing primers (E7335, NEB), and the libraries pooled and sequenced on the MiSeq system (Illumina) using pair-end reads of 300 bp.
GEO Accession
GSM2633805