GSM5718068: RNA-seq of lung tissue from DMB3 Rat; Rattus norvegicus; RNA-Seq
Total RNA was isolated from lung tissue of MCT-PH rats with or without DMB treatment using QIAzol Lysis Reagent (20874, QIAGEN Sciences, Maryland, USA). To enrich for mRNA and noncoding RNAs (ncRNAs), ribosomal RNA (rRNA) was removed from total RNA using rRNA Depletion Kit v2 (E7400L, NEBNext, MA, USA). RNA libraries were prepared for sequencing using standard Illumina protocols