5'-enriched RNA-seq of Streptomyces lividans TK24: mixed sample
S. lividans TK24 was grown in minimal media with and without casamino acids in a 1L fermenter. Samples were harvested in the early log, late log, and stationary phase . After total RNA extraction, the 6 samples were mixed in equal amounts. Stable RNA removed using the RiboZero Bacteria kit. The sequencing library was created using the protocol established by Pfeifer-Sancar et al., 2013 (PMID: 24341750).