Illumina HiSeq X (Illumina, San Diego, CA, USA) 150 bp paired-end sequencing of Rangifer tarandus: faecal sample from Cold Lake, Alberta (24461)
DNA was extracted from the mucosal coat on the faecal pellets. The DNA was normalized to 20ng/l at a final volume of 22ng/l at a final volume of 50l and shipped to The Centre for Applied Genomics (TCAG) at the Hospital for Sick Children (Toronto, Ontario) for library preparation and sequencing. The library preparation was done using the Truseq PCR-free DNA Library Preparation Kit (Illumina, San Diego, CA, USA) following the manufacturers recommended protocol, using 700 ng of DNA as input material. Validated libraries were pooled in equimolar quantities. The samples were run alongside other samples (for a total of 16 samples) on 8 lanes of an Illumina HiSeq X (Illumina, San Diego, CA, USA) 150 bp paired-end.