RNAseq
Lung samples were collected at the time of euthanasia and stored in RNAlater RNA storage reagent (Qiagen; Venlo, Limburg). Lung samples collected at the time of euthanasia were also stored in both liquid nitrogen and 10% buffered formalin for further analysis. RNA was extracted directly from lung samples stored in RNAlater (Qiagen; Hilden, Germany).Fifty ng of pure RNA was transcribed into cDNA using the Quanta Biosciences qScriptTM XLT cDNA Supermix kit (Quanta Biosciences; Gaithersburg, MD) according to the manufacturers instructions.