Arabidopsis_Transcript_Sequences S2
200ng of total RNA was taken for mRNA isolation, fragmentation and priming. Fragmented and primed mRNA was further subjected to first strand synthesis in the presence of Actinomycin D followed by second strand synthesis. The double stranded cDNA was purified using HighPrep magnetic beads. Purified cDNA was end-repaired, adenylated and ligated to Illumina multiplex barcode adapters as per RNA Library Prep Kit protocol. Adapter ligated cDNA was purified using HighPrep beads and was subjected to 15 cycles of Indexing-PCR to enrich the adapter-ligated fragments. Final PCR product was purified with HighPrep beads, followed by library quality control check. Illumina-compatible sequencing library was quantified by Qubit fluorometer and its fragment size distribution was analyzed on Agilent 2200 Tapestation.