RNAseq
RNA was isolated from bacterial biofilms in triplicate on 3 separate occasions using Qiagen RNA Protect bacterial reagent, Qiagen QiaShredder, and Qiagen RNeasy kits (Qiagen; Hilden, Germany) according to manufacturers instructions for prokaryotic RNA. The isolated RNA was immediately transcribed into cDNA using the Quanta qScript kit (Quanta Biosciences; Gaithersburg, MD) following the manufacturers instructions.