Illumina HiSeq 2500 sequencing; YAP regulates cell mechanics by controlling focal adhesion assembly
YAP regulates cell mechanics by controlling focal adhesion assembly
CAL51 cells were cultured in DMEM/F12 complemented with 10% FBS for 48h. Chromatin was immunoprecipitated from three technical replicates using an ChIP-grade anti-YAP antibody (Cell Signaling Technologies) and following the manufacture’s instructions (PierceAgarose ChIP Kit, Thermo Fisher Scientific). Control ChIP was performed by using input DNA. Library preparation and sequencing was performed by Genomix4Life S.r.l (Baronissi, Italy). Size distribution of each ChIP DNA sample was assessed by running a 1μl aliquot on Agilent High Sensitivity DNA chip using an Agilent Technologies 2100 Bioanalyzer (Agilent Technologies). The concentration of each DNA sample was determined by using Quant-IT DNA Assay Kit- High Sensitivity and a Qubit Fluorometer (Life Technologies). 10 ng of purified ChIP DNA were used as starting material for sequencing libraries preparation.
Experimental Factor: input DNA
immunoprecipitate