control_group
All samples were uniformly resuscitated and washed twice by PBS (with 0.1% BSA), then the cells were 1 ml stain buffer (with 0.1% BSA) and counted. The viability of cells in all samples assessed >80%. Cells for all samples were labeled with sample tags (BD Human Single-Cell Multiplexing Kit, USA), counted and multiplexed, ready for single-cell capture. Single-cell capture and cDNA synthesis were performed by the BD Rhapsody Single-Cell Analysis System (BD, USA), according to the manufacturers recommendations. Libraries were sequenced on multiple runs of Illumina.