GSM5731352: PTA5; Homo sapiens; RNA-Seq
the cells were resuspended in calcium- and magnesium-free PBS containing 0.04% BSA, followed by centrifugation at 300g for 5 min. The cells were then resuspended in 1mL of ice-cold red blood cell lysis buffer.and incubated at 4 °C for 10 min, followed by the addition of 10 ml of ice-cold PBS and centrifugation at 300g for 10 min. After resuspension, the sample was filtered with a 40-μm cell strainer. Cell viability was then determined using Trypan Blue staining with an automated cell counter. RNA libraries were prepared for sequencing using standard Illumina protocols