GSM5035309: D10_5083_mLN; Macaca mulatta; RNA-Seq
CD4 T cells from mesenteric LNs and pelvic LNs in monkeys necropsied on day 0, 3, 7 and 10 following infection were enriched by negative selection through EasySep™ Non-Human Primate Custom Enrichment Kit (STEMCELL Technologies).  The enriched cells were further stained with viability dyes and flow cytometric antibodies including CD45, CD3, CD4, CD8, and CD4 T cells were sorted by BD FACSAria flow cytometer. RNA extraction was carried out using RNeasy Mini Kit (QIAGEN) according to manufacturer's instructions. A Low-Input mRNA library (Clontech SMARTer) v4 was applied and samples were sequenced on Illumina NS500 Paired-End 75 bp (PE75) at the Molecular Biology Core Facility at Dana-Farber Cancer Institute. RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM5035309