GSM5082565: 5min_Sua7; Saccharomyces cerevisiae; ChIP-Seq
For ChIP-seq, lysates were sonicated, for Mnase-seq, digested chromatin was extracted using phenol/chloroform, and for RNA-seq experiments RNA was extracted using phenol/chloroform Libraries for DNA-based experiments (ChIP-seq, Mnase-seq) were created according to manufacturer's protocols using the Nugen Ultralow v2 kit (NuGEN/Tecan, 0344); RNA-seq libraries were generated using the Nugen Ovation SoLo kit (NuGEN/Tecan, using custom anydeplete primers. Contact NuGEN for ordering) All libraries were sequenced on an Illumina HiSeq 2500 at the Fred Hutchinson Cancer Center genomics core; ChIP-seq experiments were single-end 50bp read lengths and other experiments were paired-end 50bp read length
GEO Accession
GSM5082565