GSM5082427: W.raw; Homo sapiens; RNA-Seq
1) Four samples were submitted to droplet-based scRNA-seq: the Chromium Single-cell 3′-Library, Gel Bead & Multiplex Kit and Chip Kit (10× Genomics, Pleasanton, CA, USA) were employed to construct a chromium single-cell 3′-library according to the manufacturer's instructions. Cell suspensions were loaded onto a chromium single-cell chip along with reverse transcription (RT) master mix and single-cell 3′-gel beads, attempting to obtain 2000–8000 single cells per reaction. The samples were processed using 10× Genomics V2 barcoding chemistry kits. Following cell lysis, first-strand cDNA synthesis and amplification were carried out according to the manufacturer's instructions with cDNA amplification set for 12 cycles. Libraries were sequenced on an Illumina HiSeq X Ten system. 2) Two samples were submitted to Singleron scRNA-seq: Single-cell suspensions with a concentration of 1×105 cells/mL  in PBS (HyClone) were prepared. Single-cell suspensions were then loaded onto microfluidic devices, and scRNA-seq libraries were constructed according to the Singleron GEXSCOPETM protocol by the GEXSCOPETM Single-Cell RNA Library Kit (Singleron Biotechnologies). Individual libraries were diluted to 4 nM and pooled for sequencing. Pools were sequenced on Illumina HiSeq X system. 1) Four samples were submitted to droplet-based scRNA-seq: the Chromium Single-cell 3′-Library, Gel Bead & Multiplex Kit and Chip Kit (10× Genomics, Pleasanton, CA, USA) were employed to construct a chromium single-cell 3′-library according to the manufacturer's instructions. Cell suspensions were loaded onto a chromium single-cell chip along with reverse transcription (RT) master mix and single-cell 3′-gel beads, attempting to obtain 2000–8000 single cells per reaction. The samples were processed using 10× Genomics V2 barcoding chemistry kits. Following cell lysis, first-strand cDNA synthesis and amplification were carried out according to the manufacturer's instructions with cDNA amplification set for 12 cycles. Libraries were sequenced on an Illumina HiSeq X Ten system. 2) Two samples were submitted to Singleron scRNA-seq: Single-cell suspensions with a concentration of 1×105 cells/mL  in PBS (HyClone) were prepared. Single-cell suspensions were then loaded onto microfluidic devices, and scRNA-seq libraries were constructed according to the Singleron GEXSCOPETM protocol by the GEXSCOPETM Single-Cell RNA Library Kit (Singleron Biotechnologies). Individual libraries were diluted to 4 nM and pooled for sequencing. Pools were sequenced on Illumina HiSeq X system. 10× Genomics scRNA-seq/Singleron scRNA-seq
GEO Accession
GSM5082427