GSM1581569: ChIPExo-Fnr_Fnr8myc_glucose_NH4Cl_anaerobic_2_anti-myc; Escherichia coli; ChIP-Seq
Cells were enyzmatically lysed in the presence of protease inhibitors prior to fragmentation using sonication. Protein/DNA complexes were recovered using an appropriate mouse monoclonal antibody followed by recovery using Pan Mouse IgG Dynabeads. While complexed on the magnetic bead, a series of enzymatic reactions were performed to 1) end repair, fragmented DNA, 2) ligate sequencing adpator 2, 3) nick repair, 4) lambda exonuclease treatment, 5) RecJ nuclease treatment. This was based on the method developed by Rhee et al. (doi:10.1016/j.cell.2011.11.013). IP DNA was then rleased from the complex and recovered. Library construction was based on the method described by Rhee et al. (doi:10.1016/j.cell.2011.11.013).
GEO Accession
GSM1581569