GSM5224820: RNAseq_10uMSPD_rep2; Saccharomyces cerevisiae; RNA-Seq
For RNaseq library preparation, 10 µg total RNA was incubated in Ambion Fragmentation Reagent at 70 °C for 15 min, and the reaction was stopped by adding Stop Solution (ThermoFisher). Fragments were separated by electrophoresis on a 15% polyacrylamide TBE-Urea gel; after column purification and precipitation, RNA fragments were processed for cDNA library preparation as described above for RiboSeq.
GEO Accession
GSM5224820