GSM5731232: LH140; Mus musculus; RNA-Seq
Lung tissue was placed in RNAlater (Ambion, Life Technologies, Mulgrave, VIC, Australia), and frozen at -80C. Total RNA was extracted with TRIzol (Ambion, Life Technologies, Mulgrave, VIC, Australia) followed by RNAeasy (QIAGEN) as per manufacturer's instructions. Library preparation and sequencing were performed by the Australian Genome Research Facility (AGRF). RNA libraries were prepared by AGRF for sequencing using TruSeq Stranded mRNA (single end, 100bp).