GSM5170945: scATAC-seq-Hepatocyte-BEC; Mus musculus; ATAC-seq
hepatocytes were isolated, fixed in 0.1% formaldehyde, and resuspended in PBS. Tn5 transposase produced in-house was loaded with barcoded oligo adapters for a total of 96 different combinations. Fixed cells were transposed by each barcoded Tn5 complex in a 96-well plate at 37°C for 30 minutes before quenching the reaction with EDTA. Cells were pooled and redistributed into another 96-well plate where they underwent reverse crosslinking and PCR with uniquely barcoded primers.
GEO Accession
GSM5170945