16S_bacteria_coastal_sediment: Sample F6.5.1 Bacteria-May 2008-1
"The 16S rRNA V6 hypervariable region of bacterial DNA was PCR amplified using barcoded primers. The forward primer sequence was 5'-GCCTCCCTCGCGCCATCAG-X-CAACGCGAAGAACCTTACC-3', where the first segment comprises the 454 primer A adaptor, X denotes a unique 10-nucleotide barcode sequence for each sample, followed by the 16S bacteria A-967F primer sequence. The reverse primer sequence was 5'- GCCTTGCCAGCCCGCTCAG-CGACAGCCATGCANCACCT-3', where the first segment comprises the 454 adaptor and the second segment comprises the 16S bacteria B-1046R primer sequence.  Barcoded samples were purified and mixed. Purified PCR products were sequenced at the Australian Genome Research Facility, Brisbane, Australia.  Sequence data were separated by barcode into individual samples."