MSI1 eCLIP-Seq Mus Musculus : Retina input replicate 2
Mouse retina from two month old mice (80mg per sample) were collected in ice cold PBS and crosslinked by irradiating with UV light (254nm, 200mJ/cm2) using Stratalinker 2400. The tissue was snap frozen in liquid nitrogen. The library prep was carried out as described by Van Nostrand et al (PMID 27018577), using rabbit monovlonal antibody to MSI1 (Abcam Ab52865) for the IP. The libraries were sequenced on Illumina HiSeq 4000 in two batches producing 75nt and 100nt long single end reads. On average 106 milllion reads were produced per library.