GSM5732328: activated_vehicle_4; Mus musculus; RNA-Seq
Total RNA was extracted with the Qiagen's RNeasy kit To construct libraries suitable for Illumina sequencing, the Illumina TruSeq Stranded mRNA Sample preparation protocol which includes cDNA synthesis, ligation of adapters, and amplification of indexed libraries was used. The yield and quality of the amplified libraries were analysed using Qubit by Thermo Fisher and the Agilent Tapestation.