GSM5752902: myc ChIP-seq (no SM) Repl. 3 [AGH144_17]; Saccharomyces cerevisiae; ChIP-Seq
Sonicated chromatin extracts were immunoprecipitated with anti-Gcn4 (home-made), anti-TBP (from Joseph Reese's lab), anti-myc (Sigma, Cat# 11667203001) and anti-Rpb3 (NeoClone, Cat# W0012). Immunoprecipitated chromatin were treated with proteinase K and DNA were extracted with chloroform and precipitated with iso-propanol. Libraries were prepared using DNA Library Prep Kit for llumina (Cat# E7370) from New England Biolabs.