Illumina HiSeq 2000 sequencing; YD embryos 8,24,32 hpf
YD embryos 8,24,32 hpf
Zebrafish embryos in HANKS embryo buffer Around 5hpf, sham puncture the controls (SP) with hamilton syringe system. Trizol RNA extraction of whole embryos Truseq RNA Sample preparation Kits V2
Experimental Factor: treatment
sham-punctured embryos
Experimental Factor: time
24 hour