Illumina NovaSeq 6000 paired end sequencing; Predict 1 Study
For the creation of DNA libraries a                 PCR free method has been used. DNA is sheared and end-repaired,                 followed by adapter ligation.                The ligated adapter are Illumina compatible and libraries i5 and i7 dual                 indices are used to minimise index hopping.                Optimal fragment sizes have been selected (300-500 bp insert) and                 quality controlled.