RNA-Seq of Hasarius adansoni (jumping spider): mid-stage embryos
Poly(A) mRNA was extracted using a QuickPrep Micro mRNA purification kit (GE Healthcare) from 20 mid-stage sibling embryos with extending limbs. mRNA was fragmented using the NEBNext RNase III RNA Fragmentation Module (New England BioLabs). Sequencing libraries were constructed from fragmented RNA using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs) and NEBNext Multiplex Oligos for Illumina (Index Primers Set 1, New England BioLabs).