GSM5346098: Colony_12h_rep3; Escherichia coli; RNA-Seq
RNA was isolated by the hot phenol method (Jahn et al, J Microbiol Methods,2008) with some modifications. Prepared RNA was DNase-digested and cleaned up using RNACleanXP. Depletion of ribosomal RNA was performed using Ribo-Zero kits (Epicentre) for Gram-negative bacteria. Integrity of the prepared RNA was tested using a Bioanalyzer. Directional cDNA libraries were prepared using the NEBNext Ultra II Directional RNA Libary Prep Kit for Illumina (NEB E7760) according to the manufacturers instructions. Quality of the prepared libraries was tested using a Bioanalyzer.
GEO Accession
GSM5346098