HCT116_FLAG_Top2A_ChIPseq
DNA from ChIP was quantified with the Qubit dsDNA HS Assay Kit. To cleave off covalently bound tyrosyl residues from Top2a ChIP DNA samples, the samples were additionally treated with ExoVII (1U per 10ng of DNA) and purified by PCR purification Kit (Qiagen). Sequencing libraries were created according to the ThruPLEX DNA-seq kit protocol. Size selection was performed in the range of 200-700bp with AMPure XP beads and confirmed using the Agilent High Sensitivity DNA Kit on the Agilent 2100 Bioanalyzer. Libraries were pooled and sequenced using the NextSeq 500/550 High Output Kit v2.5. The sequencing run was Single End and Dual Index with 75 bp reads.