RNA-seq of homo sapiens: adult male skeletal muscle
Library construction and purification were performed according to the TruSeq RNA Sample Preparation Kit V2. The libraries were clustered using the HiSeq Rapid Cluster Kit V2 with a flow cell V2 and HiSeq rapid SBS V2. Sample sequencing was performed on Illumina HiSeq2500 machines (Illumina, San Diego, CA, USA) using the standard Illumina RNA-seq protocol with a read length of 2 × 100 bases.