16S rRNA metagenome of Bos taurus rumen fluid: archaeal population
Rumen fluid collected from fistulated cows was used to seed rumen continuous flow fermenters. Samples taken were processed according to standard protocols and archaeal 16S rRNA genes amplified using archaeal specific primers "AATGATACGGCGACCACCGAGATCTACAC" (forward) and "CAAGCAGAAGACGGCATACGAGAT" (reverse). After purification and QC, samples were submitted to a commercial sequence provider (Massey university Genome Service, NZ) for paired end sequencing using the Illumina MiSeq 500 cycle Kit_V2 for paired end 2 x 250bp read lengths.