RNA-Seq of Oncorhynchus mykiss: juvenile brain
Juvenile rainbow trout brains, n = 4 per treatment, were extracted and flash frozen in liquid nitrogen. Whole, individual brains were homogenized using a tissue homogenizer and total RNA extracted using an RNeasy Mini kit (Qiagen). A Nanodrop-2000c (Thermo Fisher Scientific) was used to measure RNA concentrations with RNA quality and integrity assessed on an Agilent 2100 Bioanalyzer chip. Samples used for downstream library preparation had high quality RNA Integrity Numbers (RIN > 9). RNA was diluted to 1 g and used in the NEBNext Ultra Library Prep Kit for Illumina (New England Biolabs). An Agilent Bioanalyzer 2100 system was used for assessing the quality of libraries. Paired end, 150 bp sequencing was performed on an Illumina NovoSeq platform by Novogene.