GSM5034699: scRNAseq_d4EB_dCD; Mus musculus; RNA-Seq
For single cell RNA sequencing, d4 EBs were dissociated with TripleExpress. Cells were then centrifuged and resuspended in PBS containing 0.04 % BSA. Next, cells were passed through a strainer, the cell concentration was determined and the single cell RNA sequencing libraries were prepared using the Chromium Single Cell Gene Expression (10x Genomics) according to the Single Cell 3' Reagents Kit (v3 chemistry) protocol. 10x Genomics - Single Cell 3' Reagents Kit (v3)
GEO Accession
GSM5034699