Guava leaf extract inhibits Quorum-Sensing and Chromobacterium violaceum induced lysis of human hepatoma cells : validation of the phenotype with whole transcriptome analysis-Control
Extraction of guava leaves (GLE)-> Demonstration of (i) Inhibition of QS in C.violaceum (MTCC 2656), and (ii) Inhibition of C.violaceum-induced cytotoxicity in HepG2 cells, by GLE-> Isolation of total RNA devoid of rRNA from control (cells grown without GLE) and experimental (cells grown with GLE)-> construction of cDNA followed by PCR amplification-> template preparation and sequencing run on IonTorrent-> inferring differential gene expression in control and experimental samples.