June insect replicate 2
Pseudomonas-specific 16S rRNA gut-microbiome profiling was carried out for BPH insects collected from different regions and across rice growing seasons, in India. Genomic DNA from BPH was PCR amplified using Pseudomonas-specific 16S rRNA gene-specfic primers. The fragment thus obtained was sequenced on GridION X5 (Oxford Nanopore Technologies, Oxford, UK) platform, using the SpotON flow cell (R9.4) in a 48hr sequencing protocol on MinKNOW 2.1 v18.05.5.