GSM2634291: H3.1WT H3K36me3 rep2; Mus musculus; ChIP-Seq
Chromatin of cross-linked mESCs with H3.1WT overexpressing were sonicated into near 500bp length, the chromatin solution were captured by 2ug of rabbit polyclonal anti-H3K36me3 antibody (Abclonal, Cat.#A2366, lot# 40648) The ChIP DNAs were measured with the Qubit 2.0 fluorometer dsDNA HS Assay (Thermo Fisher Scientific). The libraries were prepared using NEBNext Ultra™ DNA Library Prep Kit for Illumina (NEB, #E7370) according to the manual instruction.
GEO Accession
GSM2634291