GSM1363460: m_KO_hMSC; Homo sapiens; Bisulfite-Seq
Total DNA was extracted from the cells using the DNeasy Blood & Tissue Kit (Qiagen) following the manufacturer’s protocol For RRBS, 100-200ng of genomic DNA was digested with Msp I and purified by QIAquick PCR Purification kit (Qiagen)QIAquick kit. 50-600bp fragments were selected by 2% agarose gel. After addition of spike-in controls, samples were end-repaired, A-tailed and ligated to methylated adapters employing TruSeq DNA Sample Preparation Kit (Illumina). The ligated DNA was then treated with bisulfate using MethylCode Kit (Invitrogen) converting unmethylated cytosine (C) into uracil (U). Finally, sequencing libraries were prepared by PCR amplification with PfuTurbo C× Hotstart DNA polymerase (Agilent Technologies).
GEO Accession
GSM1363460