Illumina HiSeq 4000 sequencing; Phosphoenol-pyruvate carboxykinase isoform 2 (PCK2) causes metabolic defects in endothelial cells and angiogenesis.
Phosphoenol-pyruvate carboxykinase isoform 2 (PCK2) causes metabolic defects in endothelial cells and angiogenesis.
Ctrl and PCK2KD human ECs were grown in 5.5.mM versus 0mM glucose concentrations in 10cm dishes, and 4 days after transduction RNA was collected to perform RNAsequencing. RNA was extracted using TRIzol.  RNA libraries were created using the KAPA mRNA stranded library prep kit according to the manufacturers instructions.
Experimental Factor: compound
glucose
Experimental Factor: dose
5.5
Experimental Factor: genotype
PCK2 homozygous knockout