GSM5775600: MDAMB231_RNA-seq_sgNS_nuc_rep1; Homo sapiens; RNA-Seq
Trizol (Qiagen) was used to purify RNA using manufacturers protocols. Whole cell RNA was polyA selected whereas nuclear and cytoplasmic RNAs were rRNA depleted. Following this, RNAseq libraries were prepared using KAPA RNA HyperPrep Kit with RiboErase (HMR) (Illumina Platforms) (Kapa Biosystems), using 12 cycles of PCR amplification. Libraries were purified using AxyPrep Mag PCR Clean-up kit (Thermo Fisher Scientific). Each library was quantified using a Qubit fluorometer (Life Technologies) and the size distribution assessed using the 2100 Bioanalyzer (Agilent Technologies, Santa Clara, USA).