GSM5225019: ox-LDL-3; Mus musculus; RNA-Seq
Total RNA was isolated from ox-LDL-treated peritoneal macrophages using TRIzol. Then, RNA degradation and contamination were examined with 1% agarose gels,We checked RNA purity by using a NanoPhotometer spectrophotometer (IMPLEN, Munich, Germany), and RNA concentration was measured using the Qubit RNA Assay Kit with a Qubit 2.0 fluorometer (Life Technologies, Carlsbad, CA, USA). RNA sequencing was performed with a standard protocol using a BGISEQ-500 platform (BGI, Guangdong, China). RNA libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM5225019