ki1 developing ear methylC, plant 2
DNA was extracted from 50 mg developing ear tissue (4-6 cm stage) using a DNeasy Plant Mini Kit (Qiagen; 69104). 800 ng of DNA was used for preparing each MethylC-seq library according to the method of Urich et al (2015) Nature Protocols (except that Mag-Bind® RxnPure Plus was used instead of AMPure XP beads for purification steps). 6 cycles of PCR were used to amplify the libraries.