HiSeq X Ten paired end sequencing; Epigenetic regulation and transcriptional repression by Tet1 in the early post-implantation mouse embryo: WG Bisulfite sequencing
Epigenetic regulation and transcriptional repression by Tet1 in the early post-implantation mouse embryo: WG Bisulfite sequencing
Cells have been collected from B6 incipient congenic (N5; >95% B6) blastocyst-derived embryonic stem cell line (wt) converted in vitro to epiblast-like cells. Genomic DNA was extracted using the Promega Wizard kit, treated with RNase A (NEB) and further purified using the DNeasy Blood & Tissue Kit (Qiagen). DNA was fragmented by sonication to 200-300bp and processed in parallel bisulfite conversion with and without oxidation pre-treatment using the TrueMethyl-Seq kit (Cambridge Epigenetix, UK) according to the manufacturer’s instructions. CEGX TrueMethyl-Seq kit
Experimental Factor: wild type
genotype
Experimental Factor: oxidative whole genome bisulfite sequencing
protocol