GSM5081797: NIHAD51; Homo sapiens; RNA-Seq
Tumor frozen sections were stained using 1% cresyl violet and were microscope guided needle dissected in an RNase free collection tip under vacuum suction to enrich for tumor >75% for further analysis. RNA quality was assessed by Agilent Bioanalyzer, and tumors with RIN values <7.0 were excluded. Total RNA (approximately 100-300 ng per sample) for each resected tumor sample was poly(A)-selected, fragmented, converted to cDNA and barcoded for multiplex sequencing on the Illumina HiSeq 2500. RNA libraries were prepared for sequencing using standard Illumina protocols.
GEO Accession
GSM5081797