GSM5035546: mRNA-sequencing activated CD4 T-cells, 72 hours after activation [mRNA_72hB]; Mus musculus; RNA-Seq
Cells were then placed on ice, washed twice with PBS containing 100µg/mL CHX, and lysed with lysis buffer (1% triton in 20mM Tris 7.5, 150mM NaCl, 5mM MgCl2, 1mM dithiothreitol supplemented with 10 U/ml Turbo DNase and 100µg/ml cycloheximide). After lysis, samples were flash-frozen and stored in -80°C until further processing. mRNA samples were extracted using TRIZOL. Subsequent, Ribo-seq library generation was performed as previously described (Finkel, Y. et al. Comprehensive Annotations of Human Herpesvirus 6A and 6B Genomes Reveal Novel and Conserved Genomic Features. bioRxiv (2019) doi:10.1101/730028.)
GEO Accession
GSM5035546