A metagenomic approach to characterize milk microbiome in clinical and subclinical mastitis in cattle (Gir)
About 50ng DNA was used for generating 16S rRNA gene PCR amplicons. A sample tagging approach was used for multiplexing samples in the sequencing reaction on 454 MicroTilterplate. The 16S rRNA genes were individually amplified from each sample using a three composite pair of primers containing a unique 11-base barcode, a multiplex identifier sequence (MID), which was used to tag the PCR products from the respective samples.