RNA-seq of female parent
Total RNA was isolated from 100 mg frozen tissue pulverized in liquid nitrogen according to the protocol of Monarch Total RNA Miniprep Kit, including removal of DNA from the column with DNase I (New England Biolabs). Total RNA was quantified with Qbit 3.0, and quality was verified with the Agilent RNA Nano 6000 Kit to confirm appropriate sample RIN numbers. The total RNA samples were sent to Novogen for mRNA sequencing using Illumina's 100 bp paired end service. The results of the raw sequencing were transferred using ftp protocol.