GSM1370710: Sample 6; Gallus gallus; RNA-Seq
Chicken cochleae were dissected from E6.5 embryos.  Cochlea were divided into three approximately equal sized pieces to produce proximal, middle, and distal pieces.  Pieces from each region were pooled together and immediately transferred into three 1.5 mL eppendorf tubes containining 0.5 mL Tri Reagent RT (Molecular Research Center, Inc.).  Samples were then stored at -20C until further processing.  Samples from each section of the cochlea were then processed using Illumina mRNA-seq or TrueSeq preparation kits.  Separate biological replicates were also prepared and similarly analyzed.  mRNA was selected by oligo-dT magnetic beads and fragmented.  First-strand cDNA was then generated using random primers.  Second-strand synthesis, end repair and addition of a single A base and adaptor ligation were then performed.  Each RNA-seq library was DNA-sequenced the Illumina HiSeq 2000. libraries were made using Illumina mRNA-seq or TrueSeq preparation kits.
GEO Accession
GSM1370710