Sequences of A, B and C on- and off-target genomic sites from Hek293T cells treated with fCas9 and target sgRNAs
Sequencing from Guilinger, Thompson & Liu. Nature Biotechnology 2014.  Three new target sites (sites A, B, and C) were chosen in the human genome that each had potential genomic off-target sites containing one identical or nearly identical half-site.  The three on-target sites and their highly similar off-target sites were amplified and sequenced from Hek293 cells treated with a mock transfection of a fCas9 expression plasmid and either two sgRNAs spaced 15 bp apart targeting genomic site A, two sgRNA spaced 24 bp apart targeting genomic site B, or two sgRNA spaced 23 bp apart targeting genomic site C.  All genomic target sites (for A, B and C) were combined together for this one type of Cas9 treatment.