Single cell RNA-seq of mouse e17.0 ForebrainDorsal
Single-cell suspensions were prepared from microdissected tissue samples using enzymatic digestion followed by manual trituration. Cells were resuspended in artificial cerebrospinal fluid at 1000 cells/l and added to the reverse transcription mix aiming for 4000 cells. All downstream cDNA synthesis (12-13 PCR cycles), library preparation and sequencing were carried out according to the 10X Genomics Chromium Single Cell Kit Version 2.