GSM4594214: V_Treated_2_MCBG; Homo sapiens; RNA-Seq
Frozen tissue sections were immunostained using a rapid immunostaining protocol (<30 minutes) and F-LCM was performed using an NKI-Beteb antibody using established methods (PMID: 27193292).  Total RNA was extracted using the ARCTURUS® PicoPure® RNA Isolation Kit (ThermoFisher Scientific, #KIT0204).  Total RNA was amplified and cDNA was transcribed using the Ovation® RNA-Seq System V2 kit (NuGEN Technologies, #7102-32) After amplification and purification, the cDNA was fragmented in ~ 350 bp fragments and ligated into a sequencing library (NuGEN Technologies; Ovation Ultralow Library System). Illumina adapters were ligated to cDNA (Promega, LigaFast Kit). cDNA was purified via Am Pure magnetic bead and subjected to PCR amplification. The final libraries were sequenced with Illumina HiSeq 2000 with 4 samples/lane using a 1x50 bp format.
GEO Accession
GSM4594214