North Pacific Gyre Drifting ESP 9/9/2011 11:02 0.22-5um cDNA
RNA from preserved, frozen filters was enriched for mRNA using environment derived rRNA probes and converted to cDNA. Library preparation used the Beckman Biomek FXp with Beckman SPRIworks HT Reagent Kit for size selection and Illumina's TruSeq LT DNA adapters and multiplexing barcodes. Sequenced on an Illumina MiSeq at a read-lenght of 151bp paired-end.