GSM2632103: WT-Skin-Treg-Biological-Replicate2; Mus musculus; RNA-Seq
Mice were shaved and dorsal skin and ears were separated. Dorsal skin was cut into 2cm2 pieces, and ears were split into dorsal and ventral halves. All the fat was removed and skin was minced finely and digested for 90 minutes in complete DMEM with Liberase TL and DNase with vigorous shaking. Skin digests were filtered and immediately stained for FACS. Total RNA was extracted with Pico pure kit (Life technologies) followed by DNase treatment with the RNeasy kit (Qiagen). The SMART-Seq v4 Ultra Low Input RNA Kit (Clontech) was used according to the manufacturer's instructions and 75 bp single-end reads were sequenced on an Illumina NextSeq 500 instrument.
GEO Accession
GSM2632103