GSM1580802: small RNAs (18-29nt) from flies containing a Reporter with 4Us spaced in 26nt intervals; Drosophila melanogaster; ncRNA-Seq
total RNA was isolated with Trizol small RNAs were PAGE selected and 3' and 5' linkers was ligated. 1st strand cDNA synthesis was performed using SuperScript III (Invitrogen) and the library was amplified using the Kapa HiFi Library Amp real time Kit (Peqlab) with Illumina TruSeq compatible primers.
GEO Accession
GSM1580802