mRNA-Seq of Chinese alligator, male heart, wild, winter
Strand-specific cDNA libraries were generated from 3 mg RNA per sample, using a NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA) according to the manufacturers instructions. The index-coded samples were clustered using a TruSeq PE Cluster Kit v3-cBot-HS on a cBot Cluster Generation System (Illumina, San Diego, CA). The libraries were sequenced on an Illumina HiSeq 2500 platform, generating 127-bp paired-end reads.