GSM5183953: 394_1; Mus musculus; RNA-Seq
Microglia (CD45int CD11b+) from the hippocampi were isolated through a continuous 40% percoll gradient and sorted with FACSAria II (BD) into RLT Buffer (Qiagen). Total RNA was isolated using the RNeasy Plus Micro Kit (Qiagen) and pre-amplified with the SMARTer Ultra Low Input kit v4 (Clontech, Mountain View, CA) per manufacturer's recommendations. 150pg of cDNA was used to generate Illumina compatible sequencing libraries with the NexteraXT library preparation kit (Illumina, San Diego, CA).
GEO Accession
GSM5183953