GSM5082111: Control; Mus musculus; MBD-Seq
Genomic DNA was extracted with DNeasy spin column kit (Qiagen, USA). Extracted DNA was sonicated with a Covaris sonicator (Covaris Inc.) to obtain ~300 bp fragments. MBD2-mediated enrichment of methylated DNA was performed using the methylated DNA enrichment kit, EpiXplore (TAKARA, Japan) according to the manufacturer's instructions. MBD-Seq libraries were prepared using DNA SMART ChIP-Seq Kit (TAKARA, Japan) according to the manufacturer's instructions and validated for an average size of about 300-700 bp using a TapeStation and the Agilent High Sensitivity D1000 ScreenTape kit.
GEO Accession
GSM5082111