GSM5033118: Mpyr-cur4-Wtfemale_RNA-Rep1-152; Macrocystis pyrifera; RNA-Seq
For each sample, total RNA was extracted from 2 independent bulks of app. 1000 male individuals and 2 bulks of 1000 female individuals (two biological replicates for each sex) using the Qiagen Mini kit (http://www.qiagen.com) as previously described (Lipinska, Cormier, et al. 2015; Arun et al. 2019). RNA from wild-type male and female and variant Mpyr-13-4 line pools was extracted using the protocol described by (Apt et al. 1995). For each replicate, the RNA was quantified and cDNA was synthesized using an oligo-dT primer. The cDNA was fragmented, cloned, and sequenced by Fasteris (CH-1228 Plan-les-Ouates, Switzerland) using an Illumina Hi-seq 2000 set to generate 150-bp single-end reads.
GEO Accession
GSM5033118