GSM1853986: RPF 30min rep1; Mus musculus; RNA-Seq
The homogenized tissues and primary cultured cells were lysed with the buffer of 10 mM Tris-HCl at pH 7.4, 100 mM KCl, 5 mM MgCl2, 2mM DTT,  1% Triton X-100, 100 µg/mL cycloheximide with 1 µL protease inhibitor cocktail (Calbiochem), 2 µL RNasin (Promega) and 2 µL SUPERase In (Ambion) to maintain bindings between the ribosomes and mRNAs. the ribosome-mRNA complexes were purified by using Sephacryl S-400 columns The preparation method of libraries were based on the standard protocol of Illumina Truseq small RNA library prep.
GEO Accession
GSM1853986