Sequencing of a single male Endler's guppy (Poecilia wingei)
The library of DNA fragments for paired-end sequencing was prepared according to the "Illumina TruSeq Nano DNA Sample Preparation Protocol”. Starting material was 120ng genomic DNA extracted from one P. wingei male. The PCR amplified fragments were size selected using AMPure XP beads (Agencourt) and additional in a Bluepippin system from sage science. The library was sequenced on an Illumina HiSeq 2000 to yield approximately 50x coverage.