HiPSC-neuron RNA-Seq
The iPSC lines were donated by control patients or patients with alcohol use disorder (AUD) and differentiated through manipulation into neuronal lines. The neuronal lines were cultured for 12 weeks, after which each line was subsequently split into two sister-lines. For the experiment, one sister line was treated by media change with 50mM of ethanol daily for one week and one sister line was treated by media change with no ethanol daily for one week. RNA was harvested from both lines on the seventh day with the agent trizol. The harvested RNA was then enriched with rRNA-depletion before 100 cycles of paired amplification. The enriched and amplified RNA-sequences were then read with Illumina HiSeq 2000 and analyzed computationally.