GSM1364769: AS2404-seed1-RNAseq; Aegilops tauschii; RNA-Seq
Total RNAs were isolated using TRIzol reagent (Invitrogen Corp., Carlsbad, CA, USA) and treated with RNase-free DNase I (New England Biolabs, Ipswich, MA, USA) to remove any contaminating genomic DNA. mRNA extraction was performed using Dynabeads oligo(dT) (Dynal; Invitrogen Corp.). Double-stranded cDNAs were synthesized using reverse transcriptase (Superscript II; Invitrogen Corp.) and random hexamer primers. The cDNAs were then fragmented by nebulization, and the standard Illumina protocol was followed thereafter to create the mRNA-seq libraries. Small RNAs were prepared using a protocol published in Kasschau et al., (2007) and subjected to high-throughput sequencing.
GEO Accession
GSM1364769