RNA-seq of Triticum aestivum spikes 48 hours after Fusarium graminearum inoculation
The sample was collected 48 hours after Fusarium graminearum inoculation and consisted of pools of five individual heads/replication. Heads were immediately shock-frozen in liquid nitrogen and stored at -80C. RNA of pooled samples (100 mg) was extracted from 100 mg of ground tissue using the RNeasy plant mini kit (Qiagen, Venlo, The Netherlands) and checked on a Bio-Rad Experion automated electrophoresis unit (Hercules, CA). Illumina HiSeq 2500 strand-specific RNA-seq libraries were sequenced in the 125 bp paired-end mode by GATC Biotech (Konstanz, Germany- now part of Eurofins Scientific).