GSM5718241: mothers_M2_nucleus_accumbens; Microtus ochrogaster; RNA-Seq
Brains were dissected out, flash frozen, and stored at -80C until sectioning on a cryostat at -20C for tissue-punching of the medial preoptic nucleus, nucleus accumbens, and lateral septum. Total RNA was then extracted using TRI-Reagent according to the manufacturer's protocol (Molecular Research Center, #TR 118), followed by DNAse I treatment to remove any eventual DNA contamination and clean-up (RNA Clean & Concentrator, Zymo Research, Irvine, CA, USA). RNA-seq libraries were prepared by the Florida State University NGS Library Facility following poly(A) mRNA purification from total RNA based on magnetic beads, cDNA synthesis using random hexamers, and final amplification using barcoded primers (New England Biolabs, Ipswich, MA, USA). The resulting barcoded and unstranded libraries were quantified using a KAPA qPCR library quantification kit (KAPA Biosystems, Boston, MA, USA) and the absence of adapters or primers contamination was verified on an Agilent TapeStation (Agilent Technologies, Santa Clara, CA, USA).
GEO Accession
GSM5718241