Conserved gene sequence of Colletotrichum fructicola strain Nara gc5
For library preparation 1ug of genomic DNA was fragmented using Covaris M220 to generate mean fragment size of 350 bp. The fragmented DNA was used as the input for NEBNext Ultra II DNA Library Prep Kit for Illumina highseq (#E7645S) to generate genomic library. The fragmented DNA was subjected to end repair followed by Atailing and adapter ligation. Ampure bead-based size selection was performed to obtain the library of desired size. The size selected DNA was enriched by PCR amplification using Illumina index adapter primers. The amplified product was purified using Ampure beads to remove unused primers.