GSM5775634: 150-mut; Mus musculus; RNA-Seq
Tamoxifen was administered to P0/P1 pups as previously described.  Cochlear tissues from 6 Sox2CreER/+Jag1fl/fl and wildtype littermate controls were dissected under the dissecting microscope in ice-cold DEPC-treated PBS and stored in RNAlater (Invitrogen) at 4°C. The RNAs were purified by RNeasy Micro Kit (QIAGEN).  The quality and quantity of RNAs were measured by a 2100 bioanalyzer (Agilent). All RNAs had an RNA Integrity Number (RIN) value of 9.7-10. Illumina compatible sequencing libraries were generated by the University of Rochester Genomics Research Center. Libraries were hybridized to the Illumina flow cell and single-end reads of 100nts were generated.