GSM4405355: Input DNA; Mus musculus; ChIP-Seq
The T2A antibody was bespoke generated using a T2A synthetic peptide and is now available from CRB (Cambridge Research Biochemicals, crb2005269). Chromatin extracts from in vitro expanded ILC2 (10 million) were prepared using truChIP Chromatin Shearing kit (Covaris), with 5 min of crosslink and optimized shearing conditions (peak power 75, duty factor 10.0, cycles/burst 200, duration 300 sec) to obtain fragments of ~500bp Chromatin-antibody-bead complexes were then subjected to tagmentation, followed by DNA elution, and libraries amplification and purification, as described previously (Schmidl et al., 2015)
GEO Accession
GSM4405355