WES of homo sapiens: pancreatic cancer PDX model after gemcitabine treatment
Genomic DNA was extracted using DNeasy Tissue Kit (QIAGEN). Exome capture was performed using the SureSelect Human All Exon V5 (Agilent Technologies) according to the manufacturers instructions. The quantity of libraries were assessed by Qubit 2.0 Fluoromete. The quality and size of libraries were measured by 2100 Bioanalyzer High Sensitivity DNA Assay according to the reagent kit guide. For Illumina sequencing, the qualified libraries were applied to 2125 bp paired-end sequencing on Illumina HiSeq 2500 (Illumina).