16S DNA-Seq of Dermacentor variabilis: Female bacterial community: Sample 2011Dvar22
Barcoded eubacterial 16S primers 357F and 926R were used to amplify variable regions 5-3 from whole tick DNA extract. Invitrogen Platinum PCR SuperMix High Fidelity was used for primary PCR. Amplicons were sequenced on the Roche 454 FLX Titanium system. Library ID is formatted as follows: PlateID-PlateQuarter-SampleMID-SampleName.