AVBF_LPE20_2
We extracted total genomic DNA from 25 A. vulgare sisters using the Qiagen DNeasy Blood and Tissue kit, according to the protocol for animal tissues (3 h incubation in proteinase-K at 56C and 30 min RNAse treatment at 37C). These females are from our laboratory line BF2787 derived from wild animals sampled in Nice (France) in 1967.  Two mate pair (~20kb inserts) sequencing libraries were prepared (from pooled DNA) and sequenced as 30-150 bp.