GSM1577168: PSG7 rep2; Echinococcus canadensis; miRNA-Seq
Protoscoleces and cyst walls were carefully recovered from fertile hydatid cysts obtained from the livers of naturally infected swine and sheep. Samples were frozen in liquid nitrogen and stored at -80ºC until RNA extraction. RNA enriched in small RNAs (<200 nt) was purified from protoscoleces and cyst walls using mirVana miRNA Isolation Kit (Ambion) according to the manufacturer’s instructions. Libraries were prepared for sequencing using a NEBNext Small RNA Library Prep Set for Illumina (NEB) following the instruction’s manual with 1.5 μg of RNA enriched in small RNAs (<200 nt) as starting material.
GEO Accession
GSM1577168