GSM5597980: TB5_CVB1_ATCC_EXP2_S5; Homo sapiens; RNA-Seq
Total RNA was purified using the Allprep DNA/RNA/miRNA Universal kit (Qiagen) and treated in-column with DNase for 15 min according to the manufacturer's instructions. The quality of the total RNA was ensured with Advanced Analytical Fragment Analyzer (Advanced Analytical Technologies, Heidelberg, Germany). Libraries were prepared according to Illumina TruSeq® Stranded mRNA sample protocol. The high quality of the libraries was confirmed with Advanced Analytical Fragment Analyzer and the concentrations of the libraries were quantified with Qubit® Fluorometric Quantification kit (Life Technologies).