GSM5224934: Amygdala Control 3 RNA-seq; Rattus norvegicus; RNA-Seq
Brains were collected for the dissection of amygdala and following total RNA extraction, RNA integrity numbers (RIN) for each RNA sample were determined using an Agilent Tapestation and were all above 8.0. Samples were then used for RNA-sequencing (single-end) using the Illumina platform. The RNAseq libraries were prepared with Illumina's 'TruSeq Stranded mRNAseq Sample Prep kit'. The libraries were pooled in equimolar concentration, quantitated by qPCR and sequenced on three lanes for 101 cycles from one end of the fragments on a HiSeq2500 using a HiSeq SBS sequencing kit version 4. Fastq files were generated and demultiplexed with with the bcl2fastq v2.17.1.14 Conversion Software (Illumina). Reads are  100nt in length. Adaptors have been trimmed from the 3'-end of the reads.
GEO Accession
GSM5224934