RAD-seq of Podarcis hispanicus complex' contact zones and reference populations.
Digestion of genomic DNA with SbfI and MseI enzymes; ligation of SbfI and MseI adapter to restriction sites; amplification using indexed P5 and P7 Illumina primers (double index) in four independent PCR reactions; PCR products pooling and fragment selection between 400 and 500 bp using a 2.5% agarose gel.