RNA-Seq of Cleome gynandra: fully expanded leaf
Four hours after dawn, RNA was extracted and isolated (Plant RNeasy kit, Qiagen) from at least 100 mg of leaf material from at least three plants for each biological replicate. The amount and quality of RNA was determined using a Bionanalyzer RNA 6000 nanochip (Agilent). Total RNA libraries were prepared from poly(A)+ RNA using the Illumina standard protocol and then multiplexed on HiSeq to generate 3Gb of 90bp pair ended reads for each library. Each Run (SRR) represents a replicate dataset.