RNA-Seq of Zea mays: anther S9
The ribosomal RNA was removed from the extracted RNA by Epicentre Ribo-zeroTM rRNA. Removal Kit (Epicentre, USA). Sequencing libraries were constructed using the rRNA-depleted RNA by NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, USA) following manufacturers recommendations. After adapter ligation and cDNA synthesis, the library preparations were sequenced on an Illumina Hiseq 4000 platform and 150 bp paired-end reads were generated.