small RNA analysis of Oryza coarctata
Total RNA was isolated from the leaves of Oryza coarctata both the salinity treated  and control  saplings  using Trizol Reagent (Invitrogen, USA) according to the manufacturer’s instructions. The quantity and quality of total RNA were analyzed using Agilent 2100. Two small RNA libraries were made, one from control and another from salt treated tissue. Small RNA library was prepared as described by Hafner  et al., (2008). Briefly, approximately ~200ng of RNA sample enriched for small RNA was used as the starting material. Small RNA libraries for sequencing were constructed according to the Illumina  protocol outlined in “TruSeq Small RNA Sample Preparation Guide”.