subgingival and peri-implant microbiome: Sample IT5
"First permanent molars frequently present severe conditions in periodontitis cases. Implant restoration is an accepted means of replacing teeth lost due to periodontitis. The present investigation focused on the microbiome analysis of 10 healthy dental implants and 10 chronic periodontitis patients using 454-prosequencing of 16S rDNA. Subgingival plaque and peri-implant biofilm were sampled at the first permanent molar site before and after implant restoration. Results: Chronic periodontitis subjects showed greater bacterial diversity compared with implant subjects. The relative abundance of sixteen genera and twelve species differed significantly between implant and chronic periodontitis subjects. The genera Catonella, Desulfovibrio, Mogibacterium, Peptostreptococcus and Propionibacterium present in higher abundance in chronic periodontitis subjects, while implant subjects had a higher proportions of Brevundimonas and Pseudomonas sp. Conclusions: Our results demonstrate that implant restoration changes the oral microbiota. Besides, analysis suggests that periodontal bacteria can remain for a prolonged period of time at non-dental sites, from where they can colonize the peri-implant."