GSM1366021: HCT116_GROseq_DMSO_30min_p53+/+; Homo sapiens; OTHER
Nuclei were isolated, nuclear run on was preformed with BrUTP, RNA was isolated using trizol, RNA was hydrolized with NaOH to a average size of 200, DNase treated and anartic Phosphate was used to remove  5´ phosphate groups. Nascent RNA was IP with anti-BrdU, then poly A tail was added to the RNA. 3' phosphates were removed using T4 PNK. The RNA was then IP again. RNA was reverse transcribed, size selected (200-650bp), circularized, relinerized, PCRed, again size selected, and sent to the Illumina HiSeq2000 instrument for 50bp read sequencing.
GEO Accession
GSM1366021