High throughput sequencing of the total RNA from Musa acuminata cv. ‘Berangan’ infected with Fusarium oxysporum f. sp. cubense race 4 (FocR4)
The mRNA content was recovered from total RNA using mRNA purification kit. The mRNA was captured twice on poly-T oligo magentic beads prior to fragmentation using fragmentation buffer. The fragmented strand was used to synthesize the first cDNA strand by priming with random hexamers. The second strand was generated and was purified using Ampure XP beads (Illumina, UK). A single adenine base was added to the 3` ends and sequencing adaptors were then ligated to the fragments and  a flow cell was used to select the range of fragments suitable for PCR amplification. The quality control analysis for the sample library and quantification of the DNA library template was performed prior to sequencing. Sequencing was carried out using an  Illumina Hi Seq™ 2000 platform.Quality control was performed on the raw reads. Basis statistics (quality, total bases, total reads and %GC) were calculated. Aligned reads were generated using TopHat reads against the reference genome of Musa acuminata (DH) Pahang (http://banana-genome.cirad.fr).