MNase-Seq of saccharomyces cerevisiae: TBP-FRB H2A nucleosomes with rapamycin: Sample TBP-FRB (+RAP) AA nucleosomes biological rep 1 technical rep 2
The Anchor Away strain TBP-FRB was grown to log phase and treated with rapamycin for one hour prior to fixation with paraformaldehyde. Mononucleosomes were released by MNase digestion and the nucleosome-associated DNA fragments were mapped by deep sequencing.