GSM5732058: JEG-3 palbociclib; Homo sapiens; RNA-Seq
RNA was isolated using the RNAeasy Mini Kit (Qiagen, Hilden, Germany) according to the manual RNA samples used for transcriptome analyses were quantified (Qubit RNA HS Assay, Thermo Fisher Scientific, Meerbusch, Germany) and quality was measured by capillary electrophoresis using the Fragment Analyzer and the 'Total RNA Standard Sensitivity Assay' (Agilent Technologies Inc., Santa Clara, USA). The library preparation was performed according to the manufacturer's protocol ('VAHTS Stranded mRNA-Seq Library Prep Kit'). 300 ng total RNA were used for mRNA capturing, fragmentation, synthesis of cDNA, adapter ligation, and library amplification. Bead purified libraries were normalized. RNA sequencing performed on the HiSeq 3000/4000 system (Illumina Inc., San Diego, USA) with a read setup of 1x150 basepairs (bp).