GSM5033696: Oxford Nanopore Long read sequencing of Nuclear RNA from CASMC -Rep2; Homo sapiens; RNA-Seq
Nuclear RNA was extracted using the PARIS™ Kit (Thermo Fisher). Libraries were prepared using cDNA-PCR Sequencing kit from Oxford Nanopoire Technologies (SQK-PCS109) following manufacturer's instructions. Briefly, ribosomal RNA depletion was performed with Ribominus Eukaryote System V2 (Thermo Fisher) and 296ng of RNA was used for polyadenylation step using Lucigen Poly(A) Polymerase Tailing Kit (Lucigen). Following RNA purification with Agencourt RNA cleanup XP Kit (Beckman Coulter), 5ng of polyadenylated RNA was used for Nanopore library preparation using cDNA-PCR Sequencing kit (SQK-PCS109) and sequenced using Oxford Nanopore Technologies' (ONT) MinION sequencer using FLO-MIN106 flow cell. Oxford Nanopore Technology sequencing- MinION
GEO Accession
GSM5033696