RNA Seq of Gammarus minus, Organ Cave Male #1, whole animal
Individual RNA libraries were constructed using the TruSeq RNA Sample Preparation Kit (Illumina, Inc.) using total RNA from each specimen. Briefly, the TruSeq RNA library construction protocol involved the purification of poly(A)-containing mRNA molecules from total RNA using oligo-dT attached magnetic beads, followed by RNA fragmentation, cDNA synthesis, adaptor ligation, and the addition of unique indexing barcodes to each library. Paired-end 2x100 sequencing of RNA libraries was performed on the Illumina HiSeqTM 2000 next-generation sequencing system.