Metagenome of Candidatus Accumulibacter enrichment culture
DNA pair-end library is constructed with insert size of 270bp-300bp. 1.5_g DNA is fragmented by Covaris, then use the Gel-Electrophotometric to test the fragmented DNA. The fragmented DNA is combined with End Repair Mix, incubate at 20ĄC for 30 min. Purify the end-repaired DNA with QIAquick PCR Purification Kit, then add A-Tailing Mix, incubate at 37ĄC for 30 min. Combine the purified Adenylate 3'Ends DNA, Adapter and Ligation Mix, incubate the ligation reaction at 20ĄC for 15 min. Adapter-ligated DNA is selected, recovered and purified. Several rounds of PCR amplification with PCR Primer Cocktail and PCR Master Mix are performed to enrich the Adapter-ligated DNA fragments. The PCR products are selected, recovered and purified again.