GSM5642972: ssCAGE, Heart, (Sample 69); Homo sapiens; RNA-Seq
Explanted hearts were arrested with the cardioplegic solution and cooled down to +4 °C for transportation. 0.5 cm3 pieces were collected from the left and right atrial free wall, right and left ventricular base, and inferior and superior sinoatrial node. The tissue was flash frozen or stored in RNAlater (Invitrogen) overnight at +4 °C and stored at -80 °C. Total mRNA was extracted from 40 mg of tissue with RNeasy Fibrous Tissue Mini Kit (Qiagen). RNA purity and yield were quality checked with Agilent Bioanalyzer to satisfy RIN >7 and total RNA amount 3-5 μg. Total RNA was submitted for no-amplification non-tagging CAGE (nAnT-iCAGE) library preparation for the first 13 samples; the rest of the samples were processed through recent single strand (ss) CAGE protocol (Morioka et al., Methods in Molecular Biology, 2020).
GEO Accession
GSM5642972