GSM5224815: riboseq_10uMSPD_rep1; Saccharomyces cerevisiae; OTHER
Ribosome profiling (Cell filteration, Freeze-Mill lysis, RNase I footprinting, size selection, RNA extraction, preadenylated linker ligation, sample pooling, reverse transcription, cDNA circularization, PCR amplification) was done as described in "Transcriptome-wide measurement of translation by ribosome profiling" Nicholas J. McGlincy et al. Methods. 2017 August 15; 126: 112–129. doi:10.1016/j.ymeth.2017.05.028.
GEO Accession
GSM5224815