16S amplicon sequencing of carbonate precipitate from alkaline mine drainage impacted stream
Ceramic tiles were placed in a Kentucky stream impacted by alkaline mine spoil drainage to collect carbonate (aragonite) precipitate. DNA was extracted from the aragonite using a protocol designed for DNA extraction from concrete samples. 16S amplicons were generated by PCR (primers 357F and 806R) and sequenced on an Illumina MiSeq.