GSM3301759: ko_hfd_exp2_rep4; Mus musculus; RNA-Seq
Total RNA was extracted using RNA spin columns (PureLink RNA Mini Kit, Ambion). RNA quantification was carried out using Qubit Fluorimetric Quantification (Life Technologies) and 500ng of RNA were used to prepare the library. QuantSeq 3' mRNA-Seq Library Prep Kit. The procedure was initiated by oligo-dT priming, then the First-strand synthesis and RNA removal was followed by random-primed synthesis of the complementary strand. The resulting double-stranded cDNA was purified with magnetic beads, in order to remove all reaction components. The adapter sequences were added by library PCR amplification, the proper cycle number was previously determined by qPCR. The library was purified from PCR components using purification beads
GEO Accession
GSM3301759