GSM1854036: Ribosome profile NSun2 -/- 3; Mus musculus; OTHER
1 cm2 pieces of skin papilloma were snap-frozen in liquid N2 and then transferred to lysis buffer. RNAs were prepared for Ribo-seq as in Ingolia, N. T., Brar, G. A., Rouskin, S., McGeachy, A. M. & Weissman, J. S. The ribosome profiling strategy for monitoring translation in vivo by deep sequencing of ribosome-protected mRNA fragments. Nat Protoc 7, 1534-1550, doi:10.1038/nprot.2012.086 (2012). For mRNA-seq standard Illumina library preparation protocols were used.
GEO Accession
GSM1854036