GSM3294326: Sult2B1b knock-down, Batch3 (015208_A3); Homo sapiens; RNA-Seq
Priming of the 17-25µm (large) IFC plate (Fluidigm, 100-5761) was completed according to the manufacturer's instructions.  Following priming, 15µL of cell mix was added to the IFC plate along with LIVE/DEAD cell staining solution containing 4µM ethidium homodimer-1 and calcein AM (Life Technologies, L-3224).  Cells were isolated and stained within the C1 system and then manually observed on an inverted fluorescence microscope with FITC and Texas Red filters (Olympus IX51). Capture sites containing 0 or >1 cell were excluded, and cells with positive ethidium homodimer-1 staining were also excluded.  Cell lysis, reverse transcription, and cDNA amplification of single cells were conducted following the protocol for mRNA sequencing (Fluidigm). RNA sequencing by Illumina HiSeq2500 next generation sequencer
GEO Accession
GSM3294326