GSM1366011: MSC_rep1; Homo sapiens; RNA-Seq
Total RNA was prepared from MSC, HK2 cells and five of the clones obtained by limiting dilution, using TRIzol reagent according to the manufacturer’s instructions. PolyA+ RNA was obtained from 20µg of total RNA using Dynabeads® mRNA DIRECT™ Micro Kit after adding external RNA spike-in controls (ERCC). Libraries were prepared using the Ion Total RNA-Seq Kit v2. Template preparation and sequencing were performed using the Ion PGM™ Template OT2 200 Kit and the Ion PGM™ Sequencing 200 Kit v2. Each replicate was sequenced on the Ion PGM™ Sequencer using a Ion 318™ Chip.
GEO Accession
GSM1366011