GSM5033698: rpoB wild type 2; Escherichia coli; RNA-Seq
10ml samples were transferred into 15ml conical tubes containing 1.25 ml of the “stop” solution (ethanol v/v 95%, and phenol v/v 5%). Cells were then spun down and decanted. Pellets were flash frozen in liquid nitrogen and kept at -80°C until all samples were collected. Pellets were thawed on ice and resuspended in 200µl of TE buffer containing 20,000 mg/L of lysozyme and incubated at 37°C for 10 min. Total RNA was purified following a standard RNAeasy mini prep kit protocol (Qiagen, Hilden, Germany). rRNA was removed using a RiboZero kit from Illumina (Cat. # MRZB12424). The library was made using TruSeq mRNA library prep kit
GEO Accession
GSM5033698