GSM5183631: RNA-seq #1 of TET1 KO PP1 cells Batch3; Homo sapiens; RNA-Seq
Total RNA was isolated with the RNeasy Mini Kit (Qiagen, 74136). DNA was removed from RNA samples using genomic DNA eliminator spin columns. Samples were submitted to MSKCC Integrated Genomics Operation core for quality control and sequencing. After RiboGreen quantification and quality control by Agilent BioAnalyzer, 500ng of total RNA underwent polyA selection and TruSeq library preparation according to instructions provided by Illumina (TruSeq Stranded mRNA LT Kit, catalog # RS-122-2102), with 8 cycles of PCR. Samples were barcoded and run on a HiSeq 4000, with additional reads generated on a HiSeq 2500 in Rapid mode in a 50bp/50bp paired end run, using the HiSeq 3000/4000 SBS Kit or HiSeq Rapid SBS Kit v2, respectively (Illumina). An average of 54 million paired reads was generated per sample. At the most the ribosomal reads represented 1.9% of the total reads generated and the percent of mRNA bases averaged 69%.
GEO Accession
GSM5183631