16S Metagenome of Kunu beverage formulation C: Kunu product
Partial V3-V4 16S rRNA library was constructed by following the protocol described in the 16S rRNA metagenomics sequencing Library guide (Illumina, CA, USA). Briefly, the protocol included a first stage amplicon PCR step involving 0.2 M concentrations of primers 341F (forward) and 805R (reverse). Sequences were demultiplexed and depleted of Illumina barcodes and primers.