SC1C
total RNA was isolated from PBMCs using the TRIzol (Invitrogen, United States) following manufacturers protocols. During RNA isolation, DNase I treatment was additionally performed using the RNase-free DNase set (Qiagen). RNA quality was checked using an Agilent 2100 Bioanalyzer instrument, together with the 2100 Expert software and Bioanalyzer RNA 6000 pico assay (Agilent Technologies). RNA quality was reported as a score from 1 to 10, samples falling below threshold of 8.0 being omitted from the study.