GSM5082344: KO1_S3_L_scRNA-seq; Mus musculus; RNA-Seq
Testes were removed. Then an enzymatic and mechanically digestion was made to obationed a cell supension. We performed single cell isolation and cell barcoding with the Droplet-based Single-cell isolator (one-touch ddSEQ, Bio-Rad) according to the manufacturer's instructions. Single-cell-barcoded RNA-Seq libraries using Nextera technology included in the SureCell WTA 3′ Library Prep Kit (Illumina). Next generation sequencing was performed in a NextSeq (Illumina) with an attainable depth of 250,000 reads/cell
GEO Accession
GSM5082344