This project uses modified chemotaxis tube assays to identify allelic variations in the most successful bacteria in a chemotaxis competition. Metre-long, agar-filled plastic tubes were inoculated at one end with wastewater collected from the Glenelg Wastewater treatment facility, South Australia. Chemotactic bands formed in each tube and migrated away from the inoculation site. After one week, the chemotactic bands were extracted from each tube. The microbial communities used to inoculate each tube (start) and the communities present in the extracted chemotactic bands (end) were sequenced from eleven tubes to identify genetic variations which emerge as chemotactic bands migrate.
Library construction: Nextera XT (Part No. FC-131-1024)