GSM5774552: Cal27-RIP-seq-HNRNPC-input; Homo sapiens; RIP-Seq
RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1-3 ug of total RNA for the construction of sequencing libraries. RNA-seq library was prepared with approximately 1μg of total RNA using RibomovalTM rRNA Depletion Kit (Human/Mouse/Rat) (Geneseed, Guangzhou, China). Briefly, ribosomal RNA was removed from the total RNA. Next, the ribominus RNAs were fragmented and then first strand and directional second strand synthesis were performed. Then the A tailing and adapter ligation were performed with the purified cDNA. Finally, the purified, adapter-ligated DNA was amplified. The library quality and concentration was assessed by utilizing a DNA 1000 chip on an Agilent 2100 Bioanalyzer.