RNA-Seq of MAP
Wild-type MAP K-10 and MAP3916c strains were cultured to logarithmic growth stage, and RNA was extracted by Trizole.Illumina TruseqTM RNA Sample Prep Kit was used to construct the library, dTTP was replaced by dUTP in dNTPs reagent that synthesized the second strand of cDNA, so that the base in the second strand of cDNA contained A/U/C/G.Before PCR amplification, the second strand of cDNA is digested with UNG enzyme to ensure the library only contain the first strand of cDNA