IBV M41-CK passage 1
100ng of total RNA was subjected to library preparation using the NEBNEXT Ultra mRNA-Seq kit. This was performed without replicates due to restrictions in the amount of starting material available. The resulting library pools were quantified using a NEBNEXT Illumina library quantitation kit (NEB, Ipswich, MA) before being diluted and loaded onto a single lane of an Illumina HiSeq 4000 (Illumina, San Diego, CA).