GSM1368807: FLM_untreated_BS; Tuber melanosporum; Bisulfite-Seq
Genomic DNA was extracted by grinding in liquid nitrogen followed by DNeasy Plant Mini kit (QIAGEN) purification After an additional phenol:chloroform extraction, gDNA was sheared by sonication to generate DNA fragments in the 150-300 bp size range. Bisulfite treatment and library preparation were carried out as described (Feng et al. 2011), except that the EpiTect kit (QIAGEN) was utilized for bisulfite treatment. Two consecutive rounds of conversion were performed for a total of 10 hours. The resulting libraries were sequenced with  Illumina sequencing technology (Illumna Hiseq 2000 sequencer). Tissues were grinded in liquid nitrogen, extracted in the extraction solution [50% Phenol; 50% Extraction buffer (100 mM Tris-HCl pH 8.0; 100 mM NaCl; 20 mM EDTA; 1% SDS] at 65°C for 10‘ and centrifuged at 14,000 rpm for 10’. The aqueous phase was transferred to new tubes and extracted twice with phenol:chloroform (1:1) and once with chloroform. After precipitation with ethanol, samples were resuspended in H20. LiCl (2M) precipitation (2M LiCl) was employed to separate RNA (pellet) from genomic DNA (supernatant). Genomic DNA was precipitated with ethanol and resuspended in H20.
GEO Accession
GSM1368807