GSM4815953: Ribo-seq-SARS2-highMOI-48hpi-HT-rep1; Chlorocebus sabaeus; OTHER
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Infected cells were lysed and two aliquots were processed in parallel for ribo-seq and RNA-seq. For RNA-seq, total RNA was isolated using ZymoClean RNA-Clean and Concentrator kit and subjected to Illumina stranded mRNA prep. Ribo-seq libraries were prepared in house following published protocols. Both libraries were sequenced using Illumina NextSeq following the manufacturer's protocols.
GEO Accession
GSM4815953