Sequences of repeat of VEGF on- and off-target genomic sites from Hek293T cells treated with fCas9 (FokI-dCas9) and control sgRNAs
Sequencing from Guilinger, Thompson & Liu. Nature Biotechnology 2014.  The VEGF target site and its corresponding known off-target site, VEG_Off1, were amplified from genomic DNA isolated from HEK293 cells treated with fCas9, two sgRNAs targeting GFP sites as negative controls.  This was a repeat of the experiment with a larger input DNA samples and a greater number of sequence reads (150 versus 600 ng genomic DNA and > 8 x 105 versus > 23 x 105 reads for the initial and second trial, respectively) to detect off-target cleavage at this site by Cas9 nickase or fCas9.