GSM1579202: Asthma_667 (sncRNA); Homo sapiens; ncRNA-Seq
RNA isolation was performed with mirVana miRNA isolation kit (Ambion, Life Science, Zug, Switzerland). RNA concentration of each sample was evaluated with a ND-1000 spectrophotometer (NanoDrop) and its quality was analysed with the Agilent 2100 Bioanalyzer using Agilent RNA 6000 nano kit (Agilent Technologies, Milano, Italy). For small RNA profiling, 1 µg of total RNA was used for library preparation according to the Illumina TruSeq small RNA sample preparation protocol. Sized microRNA libraries were gel purified and sequenced on a Illumina HiSeq1500 sequencer. For polyA RNA sequencing, an equimolar RNA pool was prepared from all 14 RNA samples extracted from both asthmatic and normal primary BSM cells. 1 µg of total RNA was used for library preparation according to the Illumina TruSeq stranded mRNA sample preparation protocol. Resulting mRNA library was sequenced on a Illumina NextSeq sequencer.
GEO Accession
GSM1579202