GSM5035270: patient1_time_1 (positive6); Homo sapiens; RNA-Seq
RNA were extracted from white blood cells using Direct-zol RNA Miniprep Kits (Zymo Research, R2050). The eluted product was incubated with DNase I (ThermoFisher Scientific, RNase-free, EN0521) at 37 °C for 30 min to further remove the DNA and then fragmented with RNA Fragmentation Reagents (Invitrogen, AM8740) at 70 °C for 15 min. Finally, the RNA was purified with RNA Clean and ConcentratorTM-5 kits The RNA without enrichment and the m6A-enriched RNA were used for library preparation performed with SMARTer® Stranded Total RNA-Seq Kit v2 (Takara Bio USA, Inc., 634411). All of the quality-ensured libraries were sequenced on the Illumina hiseq Xten platform in 150 bp paired-end mode (sequenced by Genewiz).
GEO Accession
GSM5035270