GSM4594414: H3K36me3-WT; Mus musculus; ChIP-Seq
Intestinal epithelial cells were crosslinked with 1 % formaldehyde for 10 min at room temperature and quenched with 125 mM glycine. The fragmented chromatin fragments were pre-cleared and then immunoprecipitated with Protein A + G Magnetic beads coupled with anti- H3K36me3 antibodies. The DNA libraries were amplified for 15 cycles and sequenced using Illumina NextSeq 500 with single-end 1x75 as the sequencing mode.
GEO Accession
GSM4594414