GSM1581505: Fungal keratitis; Homo sapiens; miRNA-Seq
The flash frozen corneal tissue (using liquid nitrogen) was grinded to a powder with liquid nitrogen in a prechilled mortar and pestle sitting in a bed of dry ice, total RNA was isolated using mirVana miRNA isolation kit following the frozen tissue, and extremely hard tissues protocol provided in the kit. Libraries were sequenced in a Single Read 50 base run , using TruSeq SR Cluster Kit v3-cBot- HS (Catalog#GD-401-3001) for cluster generation on cBot and TruSeq SBS Kit v3-HS (Catalog# PE-401-3002) for sequencing on the Illumina HiSeq1000 platform Small RNA libraries were prepared for sequencing using Illumina standard protocols
GEO Accession
GSM1581505