GSM5576836: WT521_Total_rep2; Ustilago maydis; RNA-Seq
Extraction of nuclei from cultures in exponential phase was done based on the protocol described by Groner and Phillips (1975), for the extraction of total RNA the RNAeasy Mini kit (Qiagen) was used according to the manufacturer's instructions. TruSeq Stranded mRNA Library Preparation kit was used for the preparation of the cDNA libraries of the polyA nuclear fraction, and the Truseq Stranded Total RNA Gold Prep Kit was used for the preparation of the total nuclear fraction libraries.
GEO Accession
GSM5576836