GSM4816552: S6: Hypothalamus_Esr1cKO_Tamoxifen; Mus musculus; OTHER
prepared single cell suspensions, EvaGreen droplet generation oil (BIO-RAD, Hercules, CA, USA), and ChemGenes barcoded microparticles (ChemGenes, Wilmington, MA, USA) containing unique molecular identifiers (UMIs) and cell barcodes were co-flowed through a FlowJEM aquapel-treated Drop-seq microfluidic device (FlowJEM, Toronto, Canada) at recommended flow speeds (oil: 15,000 μl/h, cells: 4000 μl/h, and beads 4000 μl/h). After breakage of the droplets, the beads were washed and suspended in reverse transcriptase solution. Nextera DNA Library Preparation kit
GEO Accession
GSM4816552