GSM3295022: mRAG-WT2; Mus musculus; RNA-Seq
RNA was extracted using miRNeasy kit from QIAGEN, according to the manufacturer instructions 500ng of total RNA were depleted from ribosomal RNA, ligated, reverse transcribed and amplified (12 cycles) with the reagents from the Truseq Stranded Total RNA kit (illumina). Libraries were quantified with the Bioanalyzer 2100 Kit (Agilent) and Qubit (Thermo Fisher) . 4nM of libraries were then diluted and denatured according to the Illumina recommendations. Paired-end 2x150bp reads were sequenced on an Illumina Nextseq 500 sequencer.
GEO Accession
GSM3295022