RNA-Seq of sonicate fluid from joint prosthesis
Next generation sequencing cDNA libraries were constructed using the Ovation SoLo RNA-Seq System (NuGEN Technologies, San Carlos, CA) from 1 ng of input RNA, as previously described. Following cDNA synthesis and amplification, a SoLo AnyDeplete probe mix (NuGEN Technologies) was added to the libraries to specifically deplete human rRNA sequences. The resulting cDNA libraries were sequenced on an Illumina HiSeq 4000 platform, producing 100-nucleotide, paired-end reads.