GSM5034373: CON.R4; Bos taurus; RNA-Seq
Total RNA was extracted using TRIzol plus RNA Purification kit and included DNase treatment; RNA was further purified using PureLink Isolation kit as per manufacturer's instructions. cDNA libraries were prepared using the QuantSeq FWD kit (Lexogen, Vienna, Austria) for multiplexed sequencing following manufacturer's recommendations using UDI-adapter and UMI Second-Strand Synthesis modules (Lexogen). The fragment size distribution of the libraries was verified via micro-capillary gel electrophoresis on a LabChip GX system (PerkinElmer, Waltham, MA). The libraries were quantified by fluorometry on a Qubit fluorometer (Life Technologies, Carlsbad, CA), and pooled in equimolar ratios. Libraries were sequenced in one lane of the HiSeq 4000 sequencer (Illumina, San Diego, CA) with single-end 100 bp reads. OTHER - 3'-tag RNAseq
GEO Accession
GSM5034373