Subsurface boring core samples: MID
Using following PCR primers: PyroB-V2f: CTATGCGCCTTGCCAGCCCGCTCAG-AGTGGCGGACGGGTGAGTAA and PyroA-B518r: CGTATCGCCTCCCTCGCGCCATCAG-MIDn-ATTACCGCGGCTGCTGG.V2-V3 region was amplified. MIDs are as follows: MID-1 TACTCTCGTG MID-2 TAGAGACGAG MID-3 AGACGCACTC MID-4 AGCACTGTAG MID-5 ATCAGACACG MID-6 ATATCGCGAG MID-7 CGTGTCTCTA MID-8 CTCGCGTGTC MID-9 TCTCTATGCG MID-10 TGATACGTCT MID-11 CATAGTAGTG MID-12 CGAGAGATAC MID-13 ATACGACGTA MID-14 TCACGTACTA MID-15 CGTCTAGTAC MID-16 TCTACGTAGC MID-17 TACGAGTATG