WT1
The embryos were subjected to RNA isolation. Ten embryos per group (n = 10 from wild-type control embryos, n = 10 from mibnn2002 mutants and n = 10 from mibta52b mutants) were used to prepare a pooled RNA sample. Three biological replicates per group were prepared. Total RNA from pooled samples was extracted by using the mirVanaTM RNA isolation kit (Applied Biosystems) according to the manufacturers protocol as previously described. RNA quality was assessed by using the Agilent 2100 Bioanalyzer system and samples with a RNA Integrity Number (RIN) greater than 8 were used for RNA library construction.