GSM1581421: Ts65Dn MEFs Rep1; Mus musculus; RNA-Seq
Mouse embryos were collected, eviscerated, decapitated and then further treated with trypsin to obtain the MEFs. MEFs were cultured at least two passages in classical DMEM (Gibco) medium supplemented with antibiotics, glutamine and 10% fetal calf serum. Subconfluent MEFs were harvested in Trizol lysis buffer (Qiagen RNAeasy). Total RNA  was collected using the RNAeasy reagent (Qiagen) following the manufacturer’s instructions. mRNA-seq libraries were prepared from 5 ug of total RNA using the Illumina mRNA-seq Sample Preparation kit following the manufacturer’s instructions.
GEO Accession
GSM1581421