HMT-348-TM7c-JB-longread
Here, high molecular weight genomic DNA was extracted from the same saliva sample used to obtain the original draft genome, using a phenol:chloroform-based protocol, and was examined for purity, size, and concentration using a TapeStation (Agilent Technologies).  The DNA was not sheared or size selected.  A long-read library was prepared using a Ligation Sequencing Kit (Oxford Nanopore Technologies) and sequenced on a GridION using an R9.4.1 Flow Cell (Oxford Nanopore Technologies).  Basecalling, quality control, and adapter trimming were performed using Guppy (v4.0.11)/MinKNOW (v20.06.9) (Oxford Nanopore Technologies).