GSM5033733: CO40R1; Capsicum annuum; RNA-Seq
Total RNA was extracted from flowers and whole chili pepper fruits at different developmental stages using a NucleoSpin™ RNA Plant kit (MACHEREY-NAGEL) according to the manufacturer's instructions. RNA was extracted from two biological samples comprising either flowers (time 0 DAA) fruits or seedlings from 2-6 different plants. RNA quality was verified by determining the RNA Integrity Number (RIN) for each sample. Samples of total RNA were shipped to Novogene (https://en.novogene.com/) for library construction, sequencing and mapping to a reference genome. At Novogene, libraries were prepared and sequenced using the Illumina NovaSeq platform to obtain at least 20 million raw paired-end reads of 150 bp per sample.
GEO Accession
GSM5033733