RNA-Seq Lotus japonicus:cold stress
Total RNA was extracted from N2-frozen samples using a Plant Spectrum Total RNA Kit (Sigma), according to the manufacturerŐs instructions. RNA quality and quantitification were verified by agarose gel electrophoresis and spectrophotometric analysis. mRNA was purified using oligo-Dt and the synthesis of cDNA was conducted. Samples were prepared for RNA sequencing as described in TruSeq¨ RNA Sample Preparation Guide Illumina (July 2012).