RNA-Seq of common wheat: flag leaf
Eukaryotic mRNA was enriched by magnetic beads with Oligo (dT).  Using mRNA as template, the first strand of cDNA was synthesized with random hexamers, and then the second strand of cDNA was synthesized by adding buffer, dNTPs and DNA polymerase I.  Subsequently,cDNA purified by AMPure XP Beads