GSM1855585: WGBS seq WT iNKT BR1 (wt1); Mus musculus; Bisulfite-Seq
After isolation of the cells, DNA was isolated using the PureLink DNA isolation  kit (Life Technologies), according to the instructions of the manufacturer. DNA was fragmented to an average size of 200 bp using the Adaptive Focused Acoustics Covaris S2 instrument. Briefly, fully unmethylated l (lambda) DNA (Promega) was spiked into the mouse genomic DNA at a ratio of 1:200. DNA was end-repaired using the End Repair (Illumina Epicentre) and A-tailed (using Klenow fragment, NEB). Ligation of methylated adapters (NeBNext Multiplex Oligos for Illumina, NEB) was performed using Quick Ligase Kit (NEB). DNA was bisulfite-treated using the MethylCode Bisulfite Conversion Kit (Life Technologies).  The bisulfite treated DNA was amplified for 4 cycles -so as to ensure high complexity of the libraries- using Kapa HiFi Uracil+ (Kapa Biosystems).
GEO Accession
GSM1855585