metagenome sequencing of human stool sample from caesarean section born neonate on day 5 after birth
Whole metagenome shotgun sequencing was performed on libraries prepared from 100 ng DNA on a HiSeq2500 (Illumina) with an adapted protocol for low amount DNA libraries and sequencing 2 x 150 nt. Metagenomic sequencing was performed at GATC Biotech AG (Germany). Metagenomic reads were trimmed, human reads were removed and preprocessed reads were assembled using the integrated metaomic pipeline IMP