Meta-transcriptome of Montipora capitata: MC-289_T3-HiT_2998
All M. capitata nubbins were collected and glued on 7-8 May 2019. The nubbins were left to acclimate for four days and then the tanks were set to ramp up the temperatures by ~0.4C every 2 days for a total of 9 days (13-22 May 2019). Samples were held at 30.33C (mean) for the remainder of the experiment. Sample was collected on 3 June 2019. At the time of collection, each nubbin was transferred to a Whirl-pak under water, the water was drained from the Whirl-pak, and then flash-frozen in liquid nitrogen. The Qiagen AllPrep DNA/RNA/miRNA Universal Kit was used to isolate RNA, the TruSeq RNA Sample Preparation Kit v2 (Illumina) was used to prepare strand specific cDNA libraries. The protocol included poly-A selection; quality control was done using an Agilent Bioanalyer and a Thermo Fisher Qubit dsDNA BR (Broad Range) assay kit.