Ranavirus gene sequencing: eDNA pool 10A- two weeks after first tadpole RV (+)
To compare ranavirus genes between hosts and the environment over time, we PCR amplified five genes: the major capsid protein, DNA polymerase, ribonucleoside diphosphate reductase alpha- and beta subunit-like proteins, and a viral homolog of the alpha subunit of eukaryotic initiation factor 2. Viral gene amplicons were pooled and sequencing libraries were prepared with Nextera XT (Illumina). Libraries were pooled, spiked with 10% PhiX and sequenced on an Illumina MiSeq with v2 chemistry and 2x250 bp read lengths