thyroid tumor tissue
A frozen aliquot (approximately 1g) of both tissue and stool sample was frozen by liquid nitrogen four to five times grinded and generated a uniform fine powder for DNA extraction. To avoid contamination, each mortar used in the grinding process was cleaned and dried (5 hours) at approximately 200 C. The extracting process was following the mthhod of Schmidt TM, et al. (1991). Analysis of a marine picoplankton community by 16s rrna gene cloning and sequencing. J Bacteriol. 173,4371-4378.