GSM2634698: Th9 WT IP with Ig anti rabbit antibody; Mus musculus; ChIP-Seq
Crosslinking, extraction and shearing of chromatin was performed by using the Truchip chromatin shearing reagent kit from 300,000 cells by following manufacturer protocol. Then, sheared chromatin was immunoprecipitated with different antibodies by using the Active Motif's ChIP IT Kit by following manufacturer's instructions Libraries were prepared according to NEB's instructions accompanying the NEBNext Ultra DNA Library Prep Kit for Illumina. After library validation on a fragment analyzer, the purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the MiSeq device following the manufacturer's protocols.
GEO Accession
GSM2634698