Aerosol and surface microbiome of Moscow subway
To study the uncultivated metagenome, total DNA from aerosol and swab samples was isolated using a PureLink Microbiome DNA Purification Kit (Thermo Fisher Scientific, USA). Amplification of the seven most variable regions of the 16S rRNA gene (V2, V3, V4, V6, V7, V8, and V9) was performed using the Ion 16S Metagenomics Kit (Thermo Fisher Scientific, USA). Amplicon barcoding was performed using IonCode Barcode Adapters (Thermo Fisher Scientific, USA) according to the manufacturer's instructions. Sequencing was performed on an Ion Torrent S5 XL S5XL sequencer (Thermo Fisher Scientific, USA) using Ion 520 and Ion 530 Chips (Thermo Fisher Scientific, USA).