GSM4594530: H3.1K27M_K27me3_mm9_rep1; Mus musculus; ChIP-Seq
Cells were crosslinked with 1% PFA for 5 min. Lysed in (15 mM Tris, 1 mM CaCl2, 0.25% TritonX-100), treated with Mnase to generate mono-nucleosomes. Chromatin was dialyzed against RIPA buffer (10mM Tris, 1% TritonX-100, 0.1% SDS, 0.1% Na-DOC) and supernatant was incubated with antibody overnight. Antibody was caputred using dynabeads for 3 hours. IPed material was eluted by boiling the beads in 1% SDS buffer at 65 degree C, after washing 5 times. Libraries were constructed according to NEB Next Ultra protocol
GEO Accession
GSM4594530