Illumina MiSeq sequencing; qiita_ptid_3934:11423.N.9658.I.2.C
giardia infection (neonates vs. adults vs. controls
DNA from approximately 20mg fecal pellets was isolated with the QIAamp DNA Stool Mini Kit (QIAGEN) as instructed by the manufacturer with additional bead beating steps. For in-depth microbiota profiling, 16S rRNA gene V4 region amplicons from fecal DNA isolates were amplified by the 515F/806R primers and sequenced on an Illumina MiSeq platform using 2x300 bp according to established protocols (1).
barcode
TGCATACACTGG
center_name
Uniklinik RWTH Aachen
primer
AATGATACGGCGACCACCGAGATCTACAC TATGGTAATT GT GTGCCAGCMGCCGCGGTAA
target_gene
16S rRNA
target_subfragment
V4