GSM1580509: lymphoma_3; Mus musculus; RNA-Seq
Total RNA was isolated from sorted cells using the RNeasy-Mini Kit (Qiagen). mRNA was purified using Dynabeads mRNA purification kit (Invitrogen). The mRNA was then subjected to first-strand cDNA synthesis using a combination of random hexamers and oligo-dT. Second-strand synthesis was performed using dUTP instead of dTTP. The double stranded cDNA was sonicated using S220 Focused-ultrasonicator (Covaris). Sonicated cDNA was next ligated to adaptors and size selected. The size-selected cDNA was treated with Uracil-N-Glycosylase and then multiplex amplified by PCR. cDNA libraries were sequenced with a HiSeq 2500 sequencer (Illumina).
GEO Accession
GSM1580509