10x linked-read sequencing of Drosophila melanogaster strain A4 (10x bacoded reads)
DNA was extracted from a single female fly following the 10X Genomics Salting Out Method for DNA Extraction from insects. Purified DNA was size selected using a Sage Scientific Blue Pippin instrument to remove templates smaller than 15 kb. Size-selected DNA was used to generate a 10x Chromium Genome Library using a loading mass of 0.6ng. The resulting genomic linked-read library was sequenced on an Illumina NextSeq (mid-output) flowcell.