GSM5681078: LEO1 KO TT-seq rep1; Homo sapiens; OTHER
Total RNA was extracted using TRNzol according to the manufacturer's instructions; 1 μg 4-thiouracil (4TU) labeled S. cerevisiae BY4741 RNA was added to 100 μg total human RNA as spike-in; After fragmentation, biotinylation of 4sU-labeled RNA, purification of biotinylated RNA with Dynabeads M-280 streptavidin and rRNA depletion. Strand-specific RNA-seq libraries were constructed as previously described (Zhong et al., 2011). TT-seq
GEO Accession
GSM5681078