GSM5170956: ATAC-seq-HBO1 KO, Dox 28 days rep2; Mus musculus; ATAC-seq
Hepatocytes were suspended in lysis and transposition buffer (10 mM Tris-HCl (pH 7.5), 5 mM MgCl2, 1% DMF, 0.5% NP-40, and 1X illumina Tn5) for 30 min on a thermomixer at 37 °C and mixed at 300 rpm. After the reaction, the samples were placed on ice briefly and cleaned up by a QIAquick PCR purification kit following the instructions of the kit. PCR was performed to amplify the libraries for 18 cycles using the following PCR conditions: 72 °C for 5 min; 98 °C for 30 s; and thermocycling at 98 °C for 10 s, and 72 °C for 90 s; following by 72 °C 5 min. After the PCR reaction, libraries were purified with the AMPure (Beckman) beads.
GEO Accession
GSM5170956