ITS1 Amplicon sequencing of Lecidea lapicida infected with Muellerella pygmaea and its associated mycobiome
Dry material was ground using a TissueLyserII (Retsch). DNA extractions with the DNeasy Plant Mini Kit (Qiagen, Austria). Amplicons were generated using primers ITS1F (Gardes & Bruns 1993) and ITS2 (White et al. 1990). Emulsion PCR was performed using the GS Titanium MV emPCR Kit and method (Lib-L) sequencing was performed using the GS FLX Titanium Sequencing Kit XLR70