WGS of S. haematobium: Mixed-sex adult worms ONT LSK109
High-quality total genomic DNA was isolated from a pool of 50 male and female adult worm pairs of S. haematobium using the Circulomics Tissue Kit (Circulomics, Bal- timore, MD, USA) and used to construct a ligation-sequencing genomic DNA library (SQK-LSK109), according to the manufacturers protocol (Oxford Nanopore Technologies, Oxford, UK). Low molecular weight DNA was removed using the 10 kb-Short Read Eliminator (SRE) kit (Circulomics, Baltimore, MD, USA). The library was sequenced (48 h) in a flow cell (R9.4.1) using the MinION sequencer (Oxford Nanopore Technologies). Following sequencing, bases were called from HDF5 files (FAST5 format) using the program Guppy v.3.1.5 (Oxford Nanopore Technologies) and stored in the FASTQ format