GSM5084212: kidney cell 1588; Mus musculus; RNA-Seq
The glomerular fraction was dissociated for 15min at 37°C using a buffer containing collagenase IV (1mg/ml) (Thermo Fisher), pronase E (1mg/ml) (Sigma Aldrich), and DNase I (50U/ml). Single cells were collected and non-digested glomeruli went through a second round of dissociation. Then, cells were filtered through a 50-µm cup falcon and centrifuged at 2000 rpm for 5 min. The pellet was resuspended, viable cells stained using CellTracker (CMFDA-Green, Thermo Fisher) and dye-positive cells sorted to 384-well plates using BD FACSAriaTM. The SmartSeq2 methodology was used for the generation and sequencing of full length polyadenylated cDNAs Libraries for RNA-seq were prepared by using SMART-seq2 methodology
GEO Accession
GSM5084212