GSM5732832: MDN_0M_1; Homo sapiens; RNA-Seq
Total RNA was extracted from cells using TRIzol (Invitrogen) and purified using Direct-zol RNA mini kit (Zymo Research) with DNase I treatment. Following RNA purification, samples were confirmed to have a RIN value >9.0 when measured on an Agilent Bioanalyzer. Total RNA qualification, mRNA enrichment, double-stranded cDNA synthesis, end repair, poly-A and adaptor addition, fragments selection and PCR, library quality assessment and Illumina sequencing. Sequencing was performed on a NovaSeq 6000 system with 150-bp paired-end run by Novogene (Sacramento, CA).