GSM4721907: WT-Thymus; Mus musculus; RNA-Seq
Normal thymus from ~6 week-old littermate mice were removed. Single-cell suspensions were obtained by pressing them through a 40mm filter, and subsequently subjected to red blood cell lysis. Part of the single-cell suspensions of thymocytes were subjected to enrichment in immature thymocytes (CD4-CD3-) by staining with biotinylated antibodies against CD3 (#13-0031-85, ThermoFisher) and CD4 (13-0041-85, ThermoFisher), followed by magnetic depletion of antibody-labeled cells using streptavidin microbeads (Miltenyi Biotec 130-048-101). CD4−CD3−- enriched thymocytes were then mixed 1:1 with single-cell suspensions from total unenriched thymocytes single-cell RNA-seq libraries were prepared using the Chromium Single Cell 3′ v3 Reagent Kit (10x Genomics PN-1000092)
GEO Accession
GSM4721907