GSM5184062: CONT_5; Homo sapiens; RNA-Seq
SeraMir RNA isolation kit (SBI Biosciences) RNA quantification and quality were assessed by measuring the 260/280 ratio using a Nanophotometer (Implen, Munich, Germany). RNA integrity was evaluated using the Bioanalyzer 2100 (Agilent Technologies, Santa Clara, USA).  The average (S.D.) RIN value for islet samples utilized in this study was 9.8  0.28. Next, 500 nanograms of RNA were used to prepare a single-indexed strand-specific cDNA library using a TruSeq Stranded mRNA Library Prep Kit (Illumina). The resulting libraries were assessed for quantity and size distribution using a Qubit and Agilent 2100 Bioanalyzer; 1.5 pM pooled libraries were sequenced with 2×75bp paired-end configuration on an Illumina NextSeq500 using a NextSeq 500/550 High Output Kit with an average 52.6M reads. A Phred quality score (Q score) was used to measure the quality of sequencing, and >90% of the sequencing reads reached Q30 (99.9% base call accuracy).
GEO Accession
GSM5184062