GSM3291858: MM at diagnosis, sample 26 [miRNA-Seq]; Homo sapiens; miRNA-Seq
Trizol extraction of total RNA was performed according to the manufacturer's instructions (Gibco BRL). Small RNA cDNA libraries (for transcripts <30nt) were built following standard Illumina Truseq® Small RNA protocol, starting from 2 micrograms of total RNA for each sample. BluePippin system was used for fragments selection within the range of 145-160 bp. RNA-seq was performed on Illumina HiSeq platform. A 50-cycle single-reads sequencing was run to generate the tags corresponding to small-RNAs
GEO Accession
GSM3291858