RAD-seq P.italiae from Adult female - blood
genomic DNA from single individuals was digested using EcoRI and MseI and ligated to respective adapters comprising EcoRI and MseI restriction overhangs. Molecular identifier tags were added with PCR amplification. Resulting individual sample libraries were pooled and the resulting library pools were size selected for fragments between 500-600bp with gel electrophoresis and extraction of the respective size range. The resulting size selected library pools were sequenced using the NextSeq machine and the 1x75bp sequencing format