RNA-seq of postmortem brain tissue: glial nuclei from Brodmann area 9 : Sample Control_20_NeuN_negative_RNA_R2
~RNA extraction from 50 µl of nuclei suspension from FACS (containing ~5000 sorted glial  nuclei) was conducted using RNeasy Mini Kit (74104, Qiagen) and RNase-Free DNase Set (79254, Qiagen), following the manufacturer's instruction. Extracted mRNA in 30-µl volume was concentrated by ethanol precipitation and resuspended in 4.6 µl of RNase-free water. cDNA was prepared using SMART-seq2 protocol (Picelli et al., Nature Protocols 9 (2014) 171–181.) with minor modification.  ~600 pg of purified cDNA was used to produce a RNA-seq library using Nextera XT DNA Library Preparation kit (FC-131-1024, Illumina), following the manufacturer instructions.