RNA-Seq of S. tsinlingensis
PacBio data were processed using the SMRT Link 5.1 software pipeline from Pacific Biosciences. Firstly, subreads were obtained, and CCS were obtained by the correction between subreads. Then, the CCS were divided into FLNC and NFL sequences according to whether the sequences contained 5 'end primers, 3' end primers and Poly A tails; Then clustered the FLNC by ICE algorithm to obtain consensus sequence; Arrow software (https ://www.pacb.com/wp-conte nt/uploa ds/SMRT_Tools _Refer ence_Guide _v600.pdf) was used to refine the consensus isoforms using the NFL to obtain polished consensus sequences.