The construction of the double-stranded cDNA library was carried out with 1 g of total RNA using the TruSeq RNA Sample Preparation Kit v2 (Illumina, San Diego, CA, USA), following the manufactures protocol. The cDNA library quality and yield were assessed using 2100 Bioanalyzer (Agilent Technologies, CA, USA). Paired-end sequencing (2 x 150 bp) of the cDNA library was accomplished in a NextSeq 550 Sequencing System, using the kit NextSeq High Output 300 cycles Illumina, San Diego, CA, USA), according to manufacturers recommendations.