GSM1853926: 21_Minus; Mus musculus; RNA-Seq
embryos were dissected at embryonic day 9.5 and non-neural tube tissue was dissected away. Embryos were dissociated with collagenase IV/Trypsin/EDTA to generate a single cell suspension and treated with DNase. Cells were FACS sorted into two pools of GFP+ and RFP+ from the live singlet population. RNA was isolated immediately from sorted cells using the Arcturus PicoPure RNA Isolation Kit (Applied Biosystems). RNA libraries were generated from 1ug total RNA using the TruSeq RNA Sample Preparation Kit from Illumina according to the standard kit protocol
GEO Accession
GSM1853926