RNA-Seq of Human RPTE cells, polysome fraction (Mock infection)
Polysomes were obtained from whole cells according to established methods and the corresponding RNA was extracted with TRIzol (Ambion), and then purified with Direct-zol columns (Zymo Research) according to the manufacturers instructions. Resulting samples were submitted to the Genomics Research Core facility at the University of Pittsburgh for construction of paired-end (2x 75bp) RNA-seq strand specific libraries using enriched polyadenylated RNA and Illumina Sequencing.