GSM1581343: Mock_ChIP; Homo sapiens; ChIP-Seq
Binding of UTX transcription factor was measured using a ChIP protocol as previously described (Palii, 2011). Chromatin from 1x10^8 cells was fragmented to a size range of 100-300 bases with a Bioruptor (Diagenode) at 4 degrees C. Solubilized chromatin was immunoprecipitated with an antibody against UTX (home made 9838P mixed with A302-374A) or an isotype IgG control. Antibody-chromatin complexes were pulled-down using Dynabeads-Protein A, washed and eluted. After cross-link reversal and proteinase K treatment, immunoprecipitated DNA was extracted with phenol-chloroform. ChIPed DNA was amplified using the Illumina protocol with modifications described in (Palii, 2011).
GEO Accession
GSM1581343