GSM5081979: KO-4; Danio rerio; RNA-Seq
Total RNA was extracted from 7 dpci ventricular adult tissue and it was collected from adult hearts by removing atrioventricular and aortic valves using QIAzol Lysis Reagent (Qiagen; CA; USA) and miRNeasy Mini Kit (Qiagen; CA; USA). RNA was quantified and purity checked using a NanoDrop ND-1000 (Thermo Scientific; MA; USA). RNA integrity was verified using an Agilent 2100 Bioanalyzer (Agilent Technologies; CA; USA). cDNA libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs). Libraries were sequenced on a HiSeq 4000 system (Illumina) to generate 60 base single reads.
GEO Accession
GSM5081979