GRO-seq of Zea mays B73 seedlings rep1
Global run-on sequencing (GRO-seq) libraries were each made from 10 pooled day-6 aerial Zea mays seedlings in the B73 inbred. Nuclei were isolated and nascent transcripts were labelled by incorporation of 5-ethynyl-UTP which was biotinylatated by Click-iT reaction (Invitrogen Click-iT Nascent RNA Capture Kit C10365) and fractionated by NaOH. Biotinylated RNAs were captured on streptavidin beads (Invitrogen Dynabeads MyOne Strepavidin T1, 65601) and libraries were prepared using the BIOO Scientific NEXTFLEX Small RNA-Seq Kit v3 with no size selection.