GSM4943845: human_10X; Homo sapiens; RNA-Seq
Healthy human aorta sample from a 65-year-old male was dissected to remove the adventitial and intimal layers. The medial layer was cut into 1 mm2 pieces, a single cell suspension was obtained by digestion in 3 mg/mL collagenase IV and 1 U/ml elastase.  Following staining with 7-Aminoactinomycin D (7-AAD), cells were fixed in Lomant's Reagent (dithio-bis(succinimidyl propionate, DSP) at 4 °C overnight. Viable cells were isolated by FACS based on 7-AAD exclusion. Cells were de-crosslinked in dithiothreitol (DTT) at 37°C for 30 minutes. A total of 20,000 cells were loaded onto a 10X Chromium system. A total of 20,000 cells were loaded onto a 10X Chromium system and amplified cDNA libraries generated using the 3' Gene Expression v3.0 kit (10x Genomics) were sequenced using 150 cycle paired end protocol (NovaSeq, Illumina).
GEO Accession
GSM4943845