GSM5774297: TFAP2E CR 016; Mus musculus; OTHER
The vomeronasal organs of P10 OMPCre and OMPCreR26AP2 mice were isolated and dissociated into single-cell suspension using neural isolation enzyme/papain (NIE/Papain in Neurobasal Medium with 0.5mg/mL Collagenase A, 1.5mM L-cysteine and 100U/mL DNAse I) incubated at 37ºC. The dissociated cells were then washed with HBSS and reconstituted in cell freezing medium (90% FBS, 10% DMSO). Cells were frozen from room temperature to -80ºC at a -1°C/min freeze rate. CUT&RUN experiments were performed as previously described(Skene and Henikoff, 2017). CUT&RUN libraries were prepared using the NEBNext ultra II DNA library prep kit (New England Biolabs E7645) according to the kit's protocol.