Hyb-Seq data of 43 species of the plant family Caprifoliaceae and three species from closely related family Targeted loci
DNA was extracted using either a DNeasy kit (Qiagen, Valencia, California, USA) or with a modified 2 CTAB method (Doyle and Doyle 1987). Genomic DNAs were sheared by nebulization at 30 psi for 70 sec, yielding an average shear size of 500bp as measured by a Bioanalyzer High-Sensitivity Chip (Agilent Technologies, Inc., Santa Clara, California, USA). Libraries were constructed using the Illumina TruSeq library preparation kit (Illumina Inc., San Diego, California, USA) and NEXTflex DNA barcodes (Bioo Scientific, Austin, Texas, USA), standardized at 2nM, and pooled in 16-plexes prior to hybrid enrichment. Solution-based hybridization with MYbaits biotinylated RNA baits (MYcroarray, Ann Arbor, Michigan, USA) and enrichment followed Weitemier et al. (2014). The target-enriched libraries were then sequenced on an Illumina HiSeq 2000 with 150bp paired-end reads.