Metabetaeus lohena
Total RNA was isolated from a single Metabetaeus lohena individual collected from Makalawena Beach on Hawaii, USA. A cDNA library was prepared using a Clontech SMART cDNA Library Construction Kit and sequenced (100 bp PE on 1/5 flowcell lane) on an Illumina HiSeq 2000 by HudsonAlpha Institute for Biotechnology's Genomic Services Lab. A total of 57,240,274 100 bp PE reads were obtained and assembled de novo using Trinity to produce an Antecaridina lauensis transcriptome consisting of 161,396 contigs.