GSM5576998: b.NOTCH4KO1; Mus musculus; RNA-Seq
Total RNA of PKC and N4-/-PKC pancreatic tumor cell lines were extracted using the miRNeasy Mini kit (Qiagen, Venlo, Netherlands), quantified by spectrophotometry (Ultraspec2100; Amersham Pharmacia Biotech, Cambridge, UK) and reversed-transcribed with SuperScriptⅢ Reverse Transcriptase (Invitrogen, MA, USA) according to the manufacturer's protocol. The quality of purified RNA samples was determined using a Bioanalyzer 2100 (Agilent) with an RNA Pico kit. RNAs with RNA Integrity Number (RIN) values greater than 8.0 were used to generate sequencing libraries. Illumina Truseq RNA Sample Prep v2 using the LT protocol.
GEO Accession
GSM5576998