GSM3294419: siDANCR rep1; Homo sapiens; RNA-Seq
TRIzol reagent was used to extract total RNA from SUNE-1 cell line. we constructed Illumina sequencing libraries according to the modified strand-specific RNA sequencing protocol. Briefly, we isolated non-coding RNA and coding RNA from 3 μg of purified total RNA using a ribo-zero kit for rRNA removal. After isolation, we eluted and fragmented the RNA samples at 94°C for 8 min with Elute-Prime-Fragment Mix. Subsequently, we performed first-strand cDNA synthesis using SuperScriptII (Invitrogen, Carlsbad, CA. USA). The product with Second Strand Marking Master Mix were incubated at 16°C for 1 hour to synthesize the second-strand cDNA. After 3' ends adenylation, we ligated the DNA fragments with TruSeq adapters and the product was amplified using TruSeq PCR primers.
GEO Accession
GSM3294419