Soil13
0.5 g wet soil was used for soil total DNA extraction using an MP Fast DNASPIN kit. The V4-V5 regions of the bacterial 16S rRNA were amplified using the primers pairs 515F/806R. The PCR was conducted under conditions consisted of initial denaturation at 98 C for 1 min, followed by 30 cycles of 98 C for 10 s for denaturation, 50 C for 30s for annealing, 30s for 72 C for elongation, and then final extension at 72 C for 5 min.