Petunia inflata S3-haplotype Pollen Bio-replicate 2
Total RNA was isolated from S3-haplotype stage 5 pollen from Petunia inflata. Poly-A RNA was isolated from this total RNA, and fragmented. cDNA were produced using strand specific dUTP labeling.  cDNA fragments of 480bp were selected for sequencing, and enriched for 18 cycles. RNA-seq library preparation was performed as described by the Illumina TruSeq protocol.