GSM1366688: TP63_Cisplatin_replicate_1; Homo sapiens; ChIP-Seq
p63 or p53 bound DNA was enriched for by chromatin immunoprecipitation .End repaired sequencing libraries were prepared from two independent biological replicatesand matched input control using NEBNext sample preparation kit (New England biolabs)  according to manufacturers instructions.  The resulting adapter ligated DNA was subjected to 16 cycles of adapter mediated pcr using Herculase II Fusion DNA Polymerase (Agilent). The resulting libraries were size fractionated in a 2% agarose gel and purified to obtain 2-400Bp fragments.  Each library was validated using an agilent 2100 bioanalyser and sequenced on the Illumina GAII sequencer according to manufacturers instructions.
GEO Accession
GSM1366688