GSM5641458: 804E_M2; Drosophila melanogaster; RNA-Seq
Briefly, total RNA was extracted with Trizol using the RNeasy Mini Kit (Qiagen, Inc.) and ribosomal RNA was depleted from 5 ug of total RNA using the Ribo-Zero™ Gold Kit (Illumina, Inc.). Depleted mRNA was fragmented and converted to first strand cDNA. During the synthesis of second strand cDNA, dUTP instead of dTTP was incorporated to label the second strand cDNA.  Samples were processed in batches of 48 and 16 libraries were pooled randomly into 25 pools. Pooled library samples were quantified again to calculate final molarity and then denatured and diluted to 14pM following Illumina guidance. Pooled library samples were clustered on an Illumina cBot; each pool was sequenced on one lane of an Illumina HiSeq 2500 using 125 bp single-read v4 chemistry.
GEO Accession
GSM5641458