GSM3294469: H3K4me2_IP_delTxis_wtSmcx_day0; Mus musculus; ChIP-Seq
Sonicated chromatin was immunoprecipitated overnight with antibodies immobilized on Dynabeads Protein G. Illumina TruSeq DNA Library Prep ChIP-DNA was deproteinized, treated with Rnase-A, 3' end-repaired with T4 DNA polymerase and T4 Polynucleotide Kinase, 3' dA-tailed, ligated to adaptors, size selected and PCR amplified. Libraries were pooled and 280-600 bp were gel purified for sequencing.
GEO Accession
GSM3294469