MNase-Seq of saccharomyces cerevisiae: Untagged Control H2A.Z nucleosomes with rapamycin: Sample No FRB tag (+RAP) Z nucleosomes
"An Anchor Away strain without the FRB tag was grown to log phase and treated with rapamycin for one hour prior to fixation with paraformaldehyde. Mononucleosomes were released by MNase digestion and FLAG epitope-tagged H2A.Z-containing nucleosomes were quantitatively immunoprecipitated using anti-FLAG agarose and eluted using FLAG peptides. The IP fraction, enriched for H2A.Z nucleosome-associated DNA fragments, was mapped by deep sequencing."