GSM2633763: KO_E2 Brep1 Trep1; Mus musculus; RNA-Seq
Freshly isolated HSCs were sorted into Trizol and total RNA was extracted per manufacturers instructions. Ion Torrent Libraries were constructed using cDNA generated with NuGEN Ovation 2 protocol.  After Covaris shearing, Ion Torrent libraries were made with the KAPA Library Preparation Kit (KK8301) per manufacturers instructions.  Illumina libraries were constructed using SMART-Seq2 protocol (Picelli et al. 2014, Nat Protocols).
GEO Accession
GSM2633763