GSM5732810: RehNUD; Homo sapiens; OTHER
Massive Analyses of cDNA Ends (MACE-Seq) was performed by GenXPro GmbH in Frankfurt am Main using the MACE-Seq kit according to the manual of the manufacturers. Briefly, cDNA was generated from fragmented RNA with barcoded poly-A primers during reverse transcription. After second- strand synthesis and 5' adapter integration, a PCR with minimum number of cycles was used to produce a library that was sequenced on an Illumina NextSeq500 machine with 1x 75 bps.