GSM4721946: PBS3; Mus musculus; RNA-Seq
The femurs and tibiae of C57Bl/6 female mice were flushed. The single cell preparation was centrifuged (500 g, 10 min, 4 °C) and the resulting pellet resuspended in ACK buffer. After 2 minutes at room temperature, the lysis was stopped by adding more buffer and the sample was centrifuged (500 g, 10 min, 4 °C). Cells underwent MACS bead isolation using CD11b Microbeads (Miltenyi Biotec,130-049-601, Germany), which enabled the isolation of CD11b+ cells from the preparation. Total RNA was isolated using RNeasy Mini kit (74104, Qiagen). RNA libraries were prepared for sequencing using standard Illumina protocols.
GEO Accession
GSM4721946