miRNA-Seq of Zea mays: anther S9
The small RNA sequencing libraries were generated using NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB, USA) following manufacturers recommendations. After 3' and 5' adapter ligation and cDNA synthesis, DNA fragments corresponding to 140~160 bp (the length of small RNA between 18~30 nt plus the 3' and 5' adaptors) were recovered by 8% PAGE. The library preparations were sequenced on an Illumina Hiseq 2500 platform and 50 bp single-end reads were generated.