GSM5775988: hEB_D8_DMSO2; Homo sapiens; RNA-Seq
RNA was extracted with RNA extraction kit Total RNA was processed by mRNA enrichment method. MRNA enrichment: the mRNA with Polya tail was enriched by magnetic beads with oligodt. The obtained RNA was fragmented with the interrupt buffer, and the random N6 primers were reverse transcribed, and then the cDNA ⼆ strand was synthesized to form double stranded DNA. The ends of the synthesized double stranded DNA were flattened and phosphorylated at the 5 'end, the 3' end formed a protruding "a" sticky end, and then connected to a bubbly joint with protruding "t" at the 3 'end. The ligation products were amplified by PCR with specific primers. The PCR product was thermally denatured into a single strand, and then the single strand DNA was cyclized with ⼀ segment bridge primers to obtain a single strand circular DNA library. The constructed library was qualified for quality inspection and sequenced.