fungi diversity analysis: Intestinal fungi of C57/BL22mice
Genomic DNA from individual sample were extracted, evaluated, and amplified with primers ITS3F (5- GCA TCG ATG AAG AAC GCA GC-3) and ITS4R (5-TCC GCT TAT TGA TAT GC-3) targeting the ITS2 region of the ITS gene. The triplicate amplicons were mixed and purified before generating sequencing libraries. The libraries were sequenced using the Illumina Hiseq 2500 platform. Raw data sequences were deposited in the Sequence Read Archive (SRA) under the BioprojectID XXXXXXX.The paired-end raw reads were filtered, merged, and trimmed to generate high-quality tags. The tags were analyzed by usearch software and assigned to the same operational taxonomic unit (OTU) with 97% similarity.