GSM5751910: 1X Dox, Rep4; Mus musculus; ChIP-Seq
Lysate was prepared and diluted to hybridization conditions containing 3M guanidine thiocyanate, precleared by adding streptavidin-coated magnetic beads and incubating for 30 minutes at 37°C, mixed with biotin-labeled single stranded DNA capture probes, and incubated at 37°C for 2 hours. Streptavidin beads were used to capture the hybridized complexes. Next, captured chromatin complexes were eluted with RNaseH and crosslinks were reversed by adding Proteinase K to the probe-bead complexes and incubating overnight at 65°C. DNA from captures was purified and subjected to standard Illumina sequencing procedures.