RNA-Seq of S. haematobium: single adult female f2
RNA was isolated from ~100 mg of individual S. haematobium stages using the TriPure Isolation Reagent (Sigma Aldrich, St. Louis, MO, USA). All samples were DNase treated using a TURBO DNA-freeTM kit (Thermo Fisher Scientific) and messenger RNA (mRNA) was purified from total RNA using the Dynabeads mRNA Purification Kit (Thermo Fisher Scientific). The size, integrity (RNA integrity number, RIN) and concentration of RNA were determined using a 4200 TapeStation System RNA ScreenTape Assay (Agilent Technologies, Waldbronn, Germany) and a Qubit 3.0 Flourometer RNA High Sensitivity Assay (Life Technologies, Carlsbad, CA, USA). TruSeq Stranded mRNA (Illumina, San Diego, CA, USA) short-read libraries (150bp, paired-end) were prepared from all mRNA samples, according to the manufacturers instructions and sequenced on an Illumina NextSeq 500 sequencer