ONT raw fastq of Y. canariae NCTC 14382
Cells grown in Luria-Bertani (LB) broth (Sigma) for 18h at 25C and gDNA extracted with a Wizard Genomic DNA Purification Kit (Promega) for high molecular weight and quality/concentration checked by a Qubit. Library was generated following recommended ONT protocol and sequenced on an ONT MinION R9.4 flowcell (FLO-MIN106) for approximately 16h. Raw FAST5 files were basecalled w/ Guppy (v3.2.2) using high accuracy models.