RNA-Seq of Human RPTE cells (Mock infection)
RNA was extracted from whole cells with TRIzol (Ambion), and then purified with Direct-zol columns (Zymo Research) according to the manufacturers instructions. Resulting samples were submitted to the Genomics Research Core facility at the University of Pittsburgh for construction of paired-end (2x 75bp) RNA-seq strand specific libraries using enriched polyadenylated RNA and Illumina Sequencing.