GSM5576664: Phf19_KO_H3K27me3; Mus musculus; OTHER
For cuttag lysates were clarified using HyperactiveTM In-Situ ChIP Library Prep Kit for Illumina (Vazyme Biotech Co., Ltd)  TD901-TD902 and histone-DNA complexes were isolated with antibody. For H3K27me3 chipseq,  the chromatin was prepared and followed by ChIP-Seq analysis by Active Motif, Inc. using the antibody against H3K27me3. For H3K36me3 chipseq, the chromatin was prepared and followed by ChIP-Seq analysis by Shanghai Jiayin Biotechnology Co., Ltd, using the antibody against H3K36me3. For cuttag libraries were prepared according to TruePrep® Index Kit V2 for Illumina®. For H3K27me3 and H3K36me3 Chipseq, libraries were prepared for sequencing using standard Illumina protocols
GEO Accession
GSM5576664