SS09-longreads
.5 g DNA was used for library preparation employing the 1D ligation-based sequencing kit (SQK-LSK-109, Oxford Nanopore, UK) and Native Barcoding Expansion 1-12 (EXP-NBD104, Oxford Nanopore, UK), which was then sequenced with a FLO-MINSP6 R9.4.1 flow cell coupled to a MinION Mk1B sequencer (Oxford Nanopore Technologies, Oxford, UK) connected to a MinKNOW GUI version 4.3.20 device