pharynx replicate 2
We have improved tissue-specific transcriptome profiling in C. elegans optimizing mRNA-tagging for deep sequencing. We call this updated method polyA-tagging and sequencing (PAT-Seq). We have applied PAT-Seq and profiled tissue-specific mRNA from the C. elegans intestine, and two muscle tissues belonging to the pharynx and body wall of mixed stage worms. We have used NuGEN SPIA kit to prepare the tissue-specific cDNA libraries