GSM5085775: Smad3+FoxL2wt_2B; Homo sapiens; RNA-Seq
After 4 hours transfection and additional culture for 24 hours in serum free DMEM-F12, HGrC1 cells were lysed using TRIzol reagent (catalog #15596026, Thermo Fisher Scientific) and RNA was extracted with Direct-zol RNA MiniPrep kit (#R2052, Zymo Research, Irvine, CA). RNA Quality control was performed by Agilent TapeStation. RNA-seq libraries were prepared using the TruSeq Stranded mRNA Library Prep (Illumina, catalog #20020595) and sequenced using an Illumina HiSeq4000 Platform using 75-bp single-end reads, to an average of 22.9 +/- 3.4 million reads per sample.
GEO Accession
GSM5085775