RNA-Seq of mus musculus: adult male joint neutrophils
RNA was extracted from approximately 1x105 sorted Ly6G+ neutrophils with RNeasy Micro Kit (QIAGEN), and cDNA libraries were constructed with SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (TAKARA) followed by Nextera XT DNA Library Prep Kit (Illumina). Concentration of libraries were quantified with Qubit (Thermo Fisher) and sequenced with 2x75 pair-end sequencing on Illumina NextSeq 500 platform.