bacterial community in sediment from Nanfei river
Sequencing adaptors were ligased to high quality pooled DNA using Ion Xpress™ Plus Region Library Kit (Life Technologies, USA). Before high-throughput sequencing, all amplicon types were assessed for region size distribution and DNA concentration using a Bioanalyser 2100 (Agilent Technologies, USA). The samples were adjusted to a final concentration of 26pM and attached to the surface of Ion Sphere particles (ISPs) using Ion OneTouch™ 200 Template Kit v2 DL (Life Technologies, USA) according to the manufacturer's instructions. ISPs were enriched on the Ion OneTouch™ ES (Life Technologies, USA). ISPs with template were sequenced on “318” micro-chips using Ion PGM Sequencing 300 Kit under the Ion Torrent Personal Genome Machine (PGM, Life Technologies, USA) for 130 cycles (520 flows).