Illumina HiSeq 2500 paired end sequencing; RNA-seq on Hu5/KD3 cells cultured with or without DAPT at proliferating stage
RNA-seq on Hu5/KD3 cells cultured with or without DAPT at proliferating stage
Hu5/KD3 cells were seeded 5×10^4 cells per collagenⅠcoated  24-well plate, cultured with DMEM (High Gluc.) containing 20% FBS, 2% UltroserG and supplemented with penicillin(100 units/mL), streptomycin (100 units/mL), Amphotericin B (250 ng/mL), L-Glu (2 mmol/L). Next day, started to treat with 0.1% DMSO or 10 μM DAPT in 10% FBS/DMEM and cultured three days. Total RNA was extracted as one sample from three wells by using RNeasy mini kit (QIAGEN).  bcl2fastq2 (v2.17) software used for basecalling.Sequenced reads were mapped to hg19 whole genome using STAR software (ver. 2.5.2b) with parameters -twopassMode Basic, -outFilterIntronMotifs RemoveNoncanonical, -sjdbOverhang 99, -sjdbGTFfile.
Experimental Factor: compound
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