GSM5643081: sgControl_1; Mus musculus; RNA-Seq
Cas9-expressing OT-I cells expressing indicated sgRNAs were injected into wild-type mice via tail vein. At 7 days post-activation, Thy1.1+ cells from spleen were sorted using a S3e cell sorter. Total RNA was extracted with RNAprep pure Cell Kit (TIANGEN BIOTECH Cat# DP430) according to manufacturer's protocol. mRNAs were purified from total RNA using oligo(dT) attached magnetic beads and fragmented into small pieces using fragmentation reagent. cDNAs were generated by reverse transcription and PCR to produce libraries for sequencing.
GEO Accession
GSM5643081