MNase-Seq of saccharomyces cerevisiae: RPB1-FRB Total nucleosomes without rapamycin: Sample RPB1-FRB (no RAP) Total nucleosomes
The Anchor Away strain RPB1-FRB was grown to log phase and fixed with paraformaldehyde. Mononucleosomes were released by MNase digestion and the nucleosome-associated DNA fragments were mapped by deep sequencing.