GSM5663049: NSG+Thy2_Treg; Homo sapiens; RNA-Seq
Spleen was smashed through a 40 μm filter (Falcon), B cells were enriched by CD19+ microbeads (Miltenyi) and non-B cells were sorted for CD3+CD4+CD25hiCD127lo Treg cells and then flash frozen on dry ice. RNA samples were converted into cDNA libraries using the Illumina TruSeq Stranded mRNA sample preparation kit (Illumina # RS-122-2103).
GEO Accession
GSM5663049