Pyrus calleryana DNA SRA
High-quality genomic DNA was randomly interrupted by ultrasound. DNA fragments ranging from 150~800 bp were recovered by electrophoresis. T4 DNA Polymerase, Klenow DNA Polymerase and T4 PNK were used to repair cohesive ends to blunt ends. The 3 end of DNA fragment was amended including an A base and ligated to adaptors, including a 5 end T base.