GSM3294746: Sichuan Han group; Homo sapiens; miRNA-Seq
Whole blood was centrifuged for 5 min at 2500 rpm. The plasma and buffy coat layers were aspirated for initial removal of plasma, leukocytes and platelets. Mature RBCs were then passed through filter membranes for leukocyte removal and washed three times with phosphate-buffered saline (PBS) to obtain purified mature RBCs.Total RNA was extracted from 100 µl purified RBCs of each sample using the TRIzol reagent (Invitrogen, CA, USA) according to the manufacturer's protocol. The Quantity and purity of the total RNA were measured using an RNA 6000 Nano Lab Chip Kit and Bioanalyzer 2100 (Agilent, CA, USA). Approximately 1 μg of total RNA was used to prepare a small RNA library according to the TruSeq Small RNA Sample Prep Kit protocol (Illumina, CA, USA), and single-end sequencing (36 bp) was performed on an Illumina HiSeq 2500 at LC-BIO (Hangzhou, China) according to the manufacturer's recommended guideline.
GEO Accession
GSM3294746