GSM5718314: meRIP_Sham-4; Mus musculus; RIP-Seq
The purity and concentration of total RNA samples were determoned withNanoDrop ND-1000. Intact mRNA was isolated from total RNA samples using Arraystar Seq-Star ploy(A) mRNAIsolation Kit according to the manufacturer's protocol.Isolated mRNA was chemcally fragmented into 100-nucleotide-long fragments by incubation in fragmentation buffer. NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs) RiboZero Magnetic Gold Kit (Human/Mouse/Rat) (Epicentre, an Illumina Company) KAPA Stranded RNA-Seq Library Prep Kit (Illumina)