GSM1365875: Input in Ngn3+ cells; Mus musculus; ChIP-Seq
H3K27me3 ChIP using low cell numbers was as described (Xu et al., 2011).  We used chromatin from 0.5-1 X 10^5 sorted cells, pooled from many embryos harvested on multiple dates.  Five µl of H3K27me3 antibody were used with the MAGnify™ Chromatin Immunoprecipitation System (Invitrogen 49-2024). The preparations of multiplex libraries for sequencing were as per “NEBNext ChIP-Seq Library Prep Set for SOLiD” (NEB E6260S). Sequencing was performed on the SOLiD4.0 platform with input and H3K27me3-immunoprecipitated libraries.
GEO Accession
GSM1365875