GSM5643149: 1851013039_CS15_S32 [scRNA-seq]; Homo sapiens; RNA-Seq
Circulating tumor cells enriched by ClearCell FX were captured by Polaris System (Fluidigm). PolyA RNA from each single cell was reverse transcribed and cDNA amplified using SMARTer kit (SMARTer Ultra Low RNA Kit for the Fluidigm C1 System, Clontech #634936). Amplified cDNA was used as input to prepare sequencing libraries with the NexteraXT DNA Kit (Illumina #fc-131-1096) and with dual indices (PN FC-131-1002; Illumina). Paired-end NextSeq 74bp sequencing.
GEO Accession
GSM5643149