GSM3301642: NMuMG_E9_MET_d7_rep2; Mus musculus; RNA-Seq
Total RNA was isolated from the samples above using the miRNeasy Mini Kit (Qiagen, 217004) with on-column DNAse digestion according to the manufacturer's instructions. 5g of RNA was then subjected to rRNA depletion using the Ribozero magnetic gold kit (Epicentre, MRZG12324) followed by concentrating it using the  RNeasy MinElute Cleanup Kit (Qiagen, 74204). RNA-Seq libraries were sequenced SR50 with Illumina HiSeq 2500 High Output v2 kit (Illumina) on an Illumina Illumina HiSeq 2500 using protocols defined by the manufacturer
GEO Accession
GSM3301642