ChIP-seq of postmortem brain tissue: glial nuclei from Brodmann area 9 : Sample SCZ_24_treated_NeuN_negative_H3K4me3_R2
~10,000 glial nuclei were used to produce one ChIP-seq library. ChIP was carried out using multiplexed MOWChIP-seq with MNase digestion for chromatin fragmentation and anti-H3K4me3 (39915, Active Motif) antibody, following a published protocol (Zhu et al., Nature Protocols 14 (2019) 3366-3394.). ChIP-seq libraries were prepared using Accel-NGS 2S Plus DNA Library kit (Swift Biosciences, cat. no. 21024, 26396) from the purified immunoprecipitated DNA.