Lacticaseibacilli sequencing
DNA extracted from microbial cultures Genomic libraries were constructed employing the TruSeq DNA PCR-Free LT Kit (Illumina) and using 2.5g of genomic DNA, which were fragmented with a Bioruptor NGS ultrasonicator (Diagenode, USA) followed by size evaluation using Tape Station 2200 (Agilent Technologies). Library samples were loaded into a Flow Cell V3 600 cycles (Illumina) according to the technical support guide. Draft genome sequencing was performed through the genomic platform consisting of a MiSeq (Illumina, UK) at GenProbio srl (Parma, Italy) following the supplier's protocol (Illumina, UK).