GSM3300796: KO_HepG2_1_2; Homo sapiens; RNA-Seq
Total RNA was reverse transcribed using the Quantitect Transcription kit (Qiagen), according to the manufacturer's instructions Indexed libraries were prepared from purified RNA with the TruSeq Total Stranded RNA Sample Prep Kit (Illumina) according to the manufacturer's instructions. Libraries were quantified using the Agilent 2100 Bioanalyzer (Agilent Technologies). Index-tagged samples were equimolar and the overall concentration was 2 nM. The pooled samples were subject to cluster generation and sequenced using an Illumina HiSeq 2500 System (Illumina) in a 2x100 paired-end format at a final concentration of 8 pmol.
GEO Accession
GSM3300796