GSM5641719: WGBS_WT1; Mus musculus; Bisulfite-Seq
Genomic DNA degradation and contamination was validated by agarose gels. DNA purity was checked  using NanoPhotometer® spectrophotometer (IMPLEN, CA, USA). DNA concentration was measured  using Qubit® DNA Assay Kit in Qubit® 2.0 Flurometer (Life Technologies, CA, USA). A total amount of 100 ng genomic DNA spiked with 0.5 ng lambda DNA were fragmented by  sonication to 200-300 bp with Covaris S220. These DNA fragments were treated with bisulfite using  EZ DNA Methylation-GoldTM Kit (Zymo Research), and the library was constructed by Novogene  Corporation (Beijing, China). Subsequently, pair-end sequencing of sample was performed on Illumina  platform (Illumina, CA, USA). Library quality was assessed on the Agilent Bioanalyzer 2100 system.