GSM4943005: H1T2_ChIPSeq (replicate); Rattus norvegicus; ChIP-Seq
Nuclei were prepared from 35-50days old rat testis, decondensed and chromatin immunoprecipitation was done using anti-H1T2 antibodies. Immunocomplexes were captured on 50 μl protein A Dynabeads. Beads with bound immunocomplexes were washed, eluted and reverse cross-linked at 65°C overnight and DNA was recovered using Qiagen PCR purification kit. Input was prepared with 5% of the sonicated chromatin. Library DNA was purified using Qiagen DNA purification kit. Sequencing was performed with Illumina HiSeq X Ten system, and raw sequence reads containing adaptor sequences were trimmed and filtered with Phred quality threshold as 20. Read pairs falling below 20 bp after clipping were removed
GEO Accession
GSM4943005