GSM5170991: ChIP-seq-Hbo1 KO, +Dox: H3K14ac; Mus musculus; ChIP-Seq
Hepatocytes were cross-linked in 1% formaldehyde for 10 min at room temperature after which the reaction was stopped by addition of 0.125M glycine. Cells were then lysed in ChIP buffer (100 mM Tris at pH 8.6, 0.3% SDS, 1.7% Triton X-100, and 5 mM EDTA) and the chromatin was sheared with a Diagenode Bioruptor sonicator UCD-200 to obtain fragments of average size of 200-500 bp. Libraries for ChIP-sequencing were generated by using the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB) and barcoded using NEBNext Multiplex Oligos for Illumina (Index Primers Set 1, 2 or 3) (NEB) according to the manufacturer's instructions.
GEO Accession
GSM5170991