Rhodococcus qingshengii strain CL-05 genome sequence
Single-molecule real-time (SMRT) libraries were barcoded and prepared using the PacBio protocol Preparing Multiplexed Microbial Libraries Using SMRTbell Express Template Prep Kit 2.0(PacificBiosciences). DNA fragments larger than 6 kb were size-selected using BluePippin (Sage Science) for each library, and then pooled at  equimolar quantities. The average fragment size of the library was 15 kb, measured by a fragment analyzer (Advanced Analytical Technologies, Inc.). Sequencing was completed on a PacBio Sequel single-molecule sequencer in one 1M v3 LR SMRT Cell with 20-h movie. The samples were demultiplexed using Pacbio SMRT Link software.