RNA-Seq of Pleodorina japonica U 2523
mRNA was isolated using poly T beads, and Illumina libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit. The concentration of the libraries was determined fluorometrically, and sequencing was conducted on the Illumina NovaSeq 6000 platform (Illumina, Inc., San Diego, CA 92122 USA) to generate 151 bp paired-end reads.