S-CK
TheV3-V4 region of 16s rDNA was amplified in the presence with a pair of universal primers (347F: CCT ACG GRR BGC ASC AGK VRV GAA T, and 802R: GGA CTA CNV GGG TWT CTA ATC C). For sequencing library preparations, the obtained PCR products were added Index linker using PCR. Library quality was measured using the Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA) and library concentration was measured by a Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA.). Library was sequenced using an Illumina MiSeq with paired-end 300 bp reads with MiSeq Control Software (MCS) (Illumina, San Diego, CA, USA).