RNA-Seq of the 4th Endoclita signifier larvae
Total RNA was extracted from larval head using RNeasy Plus Mini Kit. mRNA samples were purified and fragmented using the TruSeq RNA Sample Preparation Kit v2-Set A. Random hexamer primers were used to synthesize the first-strand cDNA. After end repair, A-tailing, and the ligation of adaptors, the products were amplified by PCR and quantified precisely using the Qubit DNA Br Assay Kit. They were then purified using the MinElute Gel Extraction Kit  to obtain a cDNA library. The cDNA library was sequenced on the HiSeq2500 platform.