RNA-seq of Homo sapiens: HeLa cell line
Total RNA isolation, cDNA library construction and sequencing on BGISEQ-500 platform were performed at Beijing Genomics Institution. Clean reads were aligned to the human reference genome (GRCh7) using Bowtie2. Transcription levels of the genes were normalized to fragments per kilobase of exon model per million mapped reads (FPKM) using RNA-seq by Expectation Maximization (RSEM).