Whole-genome sequencing of mus musculus: adult male brain
We extracted genomic DNA using the E.Z.N.A. Tissue DNA Kit (Omega Bio-tek) and subjected it to the Illumina NovaSeq (Novogene) for whole-genome sequencing (50). We subjected raw reads (150 bp paired-end) to the Trimmomatic (version 0.32) for the trimming and filtering of low-quality reads.