NA24631
Exome libraries for 4 samples - NA24385, NA24149, NA24143, and NA24631 - were prepared using Ion AmpliSeq™ Exome RDY Kit, with a mean insert size of 215bp. Each sample was assigned a distinct barcode: IonXpress_020 for NA24385, IonXpress_022 for NA24149, IonXpress_024 for NA24143, and IonXpress_026 for NA24631. Each barcode library is diluted to 100pM. The libraries were emulsion-amplified individually and enriched using Ion OneTouch™ 2 System and Ion PI™ Template OT2 200 Kit v4. Outputs from 4 OneTouch runs for each sample were pooled together. Each sample was sequenced on 4 Ion Proton™ instruments using Ion PI™ Sequencing 200 Kit v4. BaseCalling and alignment were performed on a Torrent Suite v4.2 server. Sequencing reads with a mean read length of 190bp were mapped to human genome version hg19. Mean coverage across AmpliSeq™ Exome target regions is 256x per run, with raw read accuracy at 99%.