Avian orthoreovirus genome assembly using new R-SPA amplification method coupled with NGS
Whole genome amplification was done using a new random single primer amplification method (R-SPA). The Nextera XT DNA Sample Preparation Kit and 0.2 ng/l (1 ng total) of dsDNA were used to generate multiplexed paired-end sequencing libraries. The library pool was loaded in the flow cell of the 500 cycles MiSeq Reagent Kit v2 (Illumina, USA) and pair-end sequencing (2250 bp) was performed on the Illumina MiSeq instrument (Illumina, USA).