RNA-seq of Euprymna scolopes: Adult gill
Juvenile squid were colonize with ES114 strain or kept aposymbiotic and reared to adulthood. Tissues were dissected in RNAlater and kept at -80C until further processing. Total RNA was purified using QIAGEN RNeasy columns, immediately followed by treatment with TURBO DNase (Ambion). The Illumina TruSeq protocol v2.0, with polyA selection. 2x125bp.