Long-term study of Escherichia coli O157:H7 in cattle feedlot
Library pools diluted were run on an Illumina NextSeq with the Mid-Output Kit constructed with the TruSeq DNA PCR-Free High Throughput Library Prep Kit. Prior to library preparation, DNA was sheared to 350bp with a Covaris Sonicator S220 using Covaris microtube, DNA was obtained from 1ml of overnight growth in TSB using a QIAamp DNA mini extraction kit.