DNA-Seq of marine water
Each DNA sample was disrupted using a Covaris S2 sonicator (Woburn, MA). Sequencing libraries were generated using the TruSeq Nano DNA HT Sample Preparation Kit (Illumina, San Diego, CA). The shotgun metagenome libraries were sequenced with an Illumina NovaSeq-PE150 platform, generating 150-bp paired-end reads with a targeted throughput of 12 Gb per sample.