Triticum aestivum
Total RNA was isolated from frozen seedling tissue samples from two biological replicates using an RNA isolation kit (Qiagen) according to the manufacturer's instructions.A biological copy was created by combining leaf samples from three different plants.T. aestivum cv .C273 was used to create two distinct libraries, each with two biological replicates.The libraries were prepared using the Illumina-compatible NEBNextUltra Directional RNA Library Prep Kit (New England BioLabs, Ipswich, MA, USA).To generate 2 100 bp reads, RNA-Seq was conducted using Illumina (HiseqTM 2000) according to manufacturer procedure.