Hyb-Seq of Camptoloma canariense: leave tissue
Genomic DNA was extracted using a modified CTAB protocol (Shee et al., 2020). CTAB extracted samples were purified using Agencourt AMPure XP Bead Clean-up (Beckman Coulter, Indianapolis, IN, USA). The quality of the extraction was checked with Qubit3.0 Fluorometer (Thermo Scientific, Waltham, MA, USA). DNA samples were sent to AllGenetics (A Corua, Spain) for library preparation, target enrichment, and sequencing. DNA samples were used as input for capture library preparation protocol. Libraries were constructed using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturers instructions, but using half of the recommended volume for all reactions. Libraries were indexed for post-sequencing demultiplexing. Following library preparation, specic regions of the genome were hybridised to biotinylated oligonucleotide probes (MyBaits, ArborBiosciences), and then captured using magnetic beads coated with streptavidin (Dynabeads MyOne Streptavidin C1, Thermo Fisher Scientic). The libraries were quantied using Qubit3.0 Fluorometer and pooled in equimolar amounts. Pools were sequenced in different runs in an Illumina MiSeq PE300.