RNA-Seq of H. sapiens: adult female; Stage II Endometrial Cancer
RNA was extracted with Trizol reagent (Invitrogen, Carlsbad, CA) and subsequently purified with DNase I using RNeasy Mini Kit (Qiagen, Germantown, MD). Quantity and integrity of RNA was determined using RiboGreen on the Spectramax Gemini XPS plate reader (Molecular Devices, Mountain View, CA) and the Fragment Analyzer (Advanced Analytical Technologies, Inc, Santa Clara, CA). Total RNA input of 200ng was used for the TruSeq RNA Library Preparation Kit v2 or the TruSeq Stranded mRNA Library Preparation Kit (Illumina, San Diego, CA), and libraries were quantified by PCR using KAPA Library Quantification Kit for NGS (Kapa, Wilmington, MA). Size distribution of libraries were assessed on the Fragment Analyzer.