RNA-seq of Coptis chinensis of Anemoclema glaucifolium
Among all the 27 newly sequenced samples in this study, 21 RNAs were extracted from silica dried leaf tissues (SD-sample) and six were built from liquid nitrogen preserved leaf tissues (LN-sample). Previous studies have shown that developing young tissues are easier to extract high concentration of nuclear RNA (Johnson et al., 2012). So, in this study, young developing leaf tissues of the chosen samples were collected from the field and quickly dried with tell-tale silica gel. After completely dried, the leaf tissues were deposited in a -20  freezer