Genome Sequencing of SAR-CoV-2 from Brazilian Amazon
The  total  RNA  was  converted  to  complementary  DNA  (cDNA)  using  the cDNA  Synthesis  System  Kit  and  400  M  of  random   primers,   following   the manufacturer's  procedure.  The  reaction solution  was  purified  with  the  Agencourt AMPure  XP  Reagent.  The  cDNA  library  was  prepared  and  sequenced using the methodology described  in the Nextera XT DNA  Library Preparation  Kit on a NextSeq (Illumina,  Inc)  platform  by  paired-end  methodology  with  300  cycles  (2x150  reads),  in the Evandro Chagas Institute, Brazil Ministry of Health.