RNA-seq of homo sapiens: hCMEC/D3 cells
Total RNA was extracted using the RNeasy mini kit (QIAGEN, USA) following the manufacturers protocol. RNA concentration was quantified using Qubit RNA HS assay kit (Thermo Fisher Scientific, USA). Sequencing library was prepared using an Illumina TruSeq stranded total RNA kit (Illumina, USA). Pair-end sequencing of 150 bp was conducted using Illumina NovaSeq 6000 at Novogene in Beijing.