CM96hC3_raw
A total of 18 Beagle dogs, 6-7 weeks old, were purchased from and housed at the National Canine Laboratory Animal Resource Center (Guangzhou, China). According to the migration time point of the T. canis in definitive host, these puppies were divided into three time point groups: 24 post-infection (hpi), 96 hpi, and 36 days post-infection (dpi). Each time point group included infected group and control group. Puppies in the infected groups were infected orally with 1 mL of saline solution containing 300 infectious T. canis eggs, while puppies in the control groups were mock-infected orally with 1 mL saline only. At each of the above indicated time points post-infection, puppies were anesthetized using Zoletil 58 (Virbac, Nice, France), and then were euthanized through injecting KCl solution into the heart. Then, the tibial bone marrow was rapidly separated aseptically and was stored in liquid nitrogen for RNA extraction.