RNA-Seq of  Homo sapiens: Adult carotid artery plaques
Single-cell suspensions with 1  105 cells/ml in PBS (HyClone) were prepared. The suspensions were then loaded onto microfluidic devices and scRNA-seq libraries were constructed according to the Singleron GEXSCOPETM protocol using the GEXSCOPE Single-Cell RNA Library Kit(Singleron,1110011).After quality checks, individual libraries were diluted to 4 nM and pooled for sequencing on an Illumina HiSeq X with 150 bp paired end reads.