Small RNAseq sequencing
Samples were taken from H. salinarum NRC-1 wild strain cells at mid-log phase (OD600 = 0.4-0.5) during standard growth condition (water bath incubator at 37ºC with agitation of 125 r.p.m). Small RNAs from two biological replicates were extracted using the MirVana RNA extraction kit (Ambion). RNA (1g) was then submitted to the strand-specific TrueSeq Small protocol from Illumina, without fragmentation. In order to enrich for small RNAs, cDNA synthesized was separated in a polyacrylamide gel and molecules from 20nt to 230nt were selected. The strand-specific sequencing was performed in MiSeq apparatus per the manufacturer’s instructions.