Amplicon of phytoplankton: marine
Sequence adapters and low-quality reads removed from the paired-end reads using BBDuk from the BBTools package (https://sourceforge.net/projects/bbmap/). Then, the forward and reverse reads were merged using USEARCH v11.0.667 (https://www.drive5.com/usearch/). Sequences shorter than 150 bp or longer than 600 bp were removed from downstream processing. Reads were then aligned with the SILVA database (release 132)