symbiont ITS2 amplicon sequencing from coral fragment from colony 17NB in October
A small (~ 5cm) fragment was collected from Acropora hyacinthus colonies across three reef zones and throughout a bleaching event and preserved in ethanol. Tissue was scraped from skeleton using a razor blade and genomic DNA was extracted using an SDS digestion protocol and cleaned. DNA quantity was assessed with a Qubit and quality was assessed with gel electrophoresis before sequencing.