RNA-Seq of MDA-MB-231: SAHA of HDAC&G4 dual-targeting compound treatment-biorep3
1. Total RNA extraction. 2. Perform first strand cDNA synthesis. 2.Proceed immediately to second strand cDNA synthesis. 3. Purifiy double-stranded cDNA using DNA clean beads. 4. End prep of cDNA library. 5. Adaptor ligation. 6. Purifiy the Adaptor Ligated DNA Using Beads. 7. PCR Enrichment of Adaptor Ligated DNA. 8. Purification of the PCR reaction using beads.