WGS of Populus nigra: Basento_BS-37
To prepare the library , we followed the Illumina classical library preparation protocol ; 200 ng of genomic DNA was fragmented to approximately 200 base pairs (bp) using a Covaris ultrasonicator ; The purified adaptors compatible with sequencing on an Illumina HiSeq instrument were ligated. The resulting DNA library was purified. The sequencing was performed in paired-end reads (2 x 150 pb) on an Illumina HiSeq4000 flow-cell.