CG18549 KD 3
For RNA-sequencing approximately 1g of total RNA (diluted in water) per sample was collected from whole flies. RNA was obtained using the Trizol protocol and RNA quality was tested using with a RIN value obtained from a Bioanalyzer/TapeStation or equivalent. RNA with RIN value of 8 or higher was used for RNA sequencing. TruSeq stranded mRNA protocol with a poly-A selection was used before the library preparation. The libraries were sequenced on the NovaSeq-instrument on one lane SP. The sequencing was done from both sides of the sequencing library.