Single Cell RNA-Seq of PN1a Model at week 16
Five thousand individual cells with a viability of >88% was targeted for GEM generation and barcoding using 10x GemCodeTechnology, which allows for partitioning thousands of cells into nanoliter-scale Gel Bead-In-Emulsions (GEMs), applying ~750,000 barcodes to separately index the transcriptome each cell. Full-length barcoded cDNA was generated and amplified by PCR, followed by enzymatic fragmentation, end-repair, A-tailing, and adaptor ligation. Single cell libraries were run using paired-end sequencing with single indexing with the NextSeq 550 platform