WGS of Leishmania donovani parasites with different paromomycin resistance profiles
Genomic DNA was used to generate paired-end libraries using the NEBNext Ultra II FS DNA library prep kit for Illumina sequencing (New England Biolabs), according to manufacturers protocol, and amplified using NEBNext multiplex oligos for Illumina (dual index primers, NEB). Pooled and barcoded libraries were sequenced to 2 x 150bp on an Illumina Hiseq 3000 platform