RNA-seq of homo sapiens: adualt female oocyte 2
After the total RNA extraction and DNase I treatment, magnetic beads with Oligo (dT) are used to isolate mRNA. fragmented,  cDNA synthesized. Short fragments are purified and resolved. After that, the short fragments are connected with adapters. The suitable fragments are selected for the PCR amplification. Agilent 2100 Bioanaylzer and ABI StepOnePlus Real-Time PCR System are used in quantification and qualification of the sample library. At last, the library could be sequenced using BGISEQ-500.