DNA-Seq of isolated bare soil under early snowmelt at the Lower Montane location
DNA was extracted from the samples using the ZymoBIOMICS DNA Miniprep kit. 16S and 18S gene amplification was conducted using 5 Prime Hot MasterMix and a primer set developed by utilizing 16s rRNA region V4-V5 primers 515F_Y and 926R. Resulting amplicons were purified and normalized to equimolar concentrations. The samples were then concentrated using the 30K ultra centrifugal Amicon filters. The final concentrations were determined using the Qubit 2.0 fluorometric quantitation.