Metagenomics of human gut : HC
1g genomic DNA was randomly fragmented by Covaris. The fragmented genomic DNA were selected by Magnetic beads to an average size of 200-400bp. Fragments were end repaired and then 3 adenylated. Adaptors were ligated to the ends of these 3 adenylated fragments. This process was to amplify fragments with adaptors from previous step. PCR products were purified by the Magnetic beads. The double stranded PCR products were heat denatured and circularized by the splint oligo sequence. The single strand circle DNA (ssCir DNA) were formatted as the final library.