G16
A set of primers was used to amplify the V4 variable region of the 16S rRNA gene: CS1_515F (ACACTGACGACATGGTTCTACAGTGCCAGCMGCCGCGGTAA) CS2_806R (TACGGTAGCAGAGACTTGGTCTGGACTACHVGGGTWTCTAAT) ( Caporaso et al., 2012).Illumina MiniSeq sequencing was performed at the DNA Services (DNAS) Facility University of Illinois, Chicago (UIC). The procedure included a second PCR amplification in 96-well plates, where each well received a separate primer pair, obtained from the Access Array Barcode Library for Illumina Sequences [10-base barcode (Fluidigm, South San Francisco, CA; Item no. 100-4876)]