RNA-Seq of Chinese mitten crab: muscle from different limb regeneration stages
A total of >5 g of RNA with an RNA integrity number above 8.0 and an OD260/280 between 1.82.2 was used for RNA-Seq library construction, employing the TruseqTM RNA sample prep Kit for Illumina (NEB, USA). After quantification employing TBS380, paired-end RNA-Seq sequencing libraries were sequenced on the Illumina HiSeq 4000 (2  150 bp read length) platform.