Multiplexed Shotgun Genotyping of Teleopsis dalmanni : Backcross offspring F147
Backcross was produced by mating a male from the Gombak 2A inbred strain to a hybrid female obtained from a cross between a male from the same 2A inbred strain and a female from a noninbred population of Teleopsis dalmanni collected near Bukit Lawang, Sumatra.  249 (131 female and 118 male) individuals were prepared from the "G12" backcross.   71 male individuals were prepared from the "G21" backcross produced.   Backcross individuals and parents were individually genotyped using multiplex shotgun genotyping using the MseI enzyme, 5 indices and 48 barcode adapters (Andolfatto et al. 2011).