Corynebacterium sp. P8776
the DNA was diluted to obtain 1 ng of each genome as 45 input. The  tagmentation  step fragmented and tagged the DNA. Then, limited cycle PCR 46 amplification (12 cycles) completed the tag adapters and introduced dual-index barcodes. 47 After purification on AMPure XP beads (Beckman Coulter Inc, Fullerton, CA, USA), the 48 libraries were then normalized on specific beads according to the Nextera XT protocol 49 (Illumina). Normalized libraries were pooled into a single library for sequencing.