Rice genome sequencing: leaf of inbred variety WuxiangB
Genomic DNAs were extracted from young leaves using CTAB method . At least 5 ug of genomic DNA for each variety was sheared into 200-5000 bp using the Covaris system (Life Technologies). After end-repairing and A-tailing, DNA fragments were ligated to paired-end adaptors, and then PCR amplified with ~500 bp inserts for library construction.