viral metagenomic sequencing of nasopharyngeal swab -RNA
A sample that tested positive for seasonal influenza type A and B via rapid lateral flow immunoassay (Abbot BinaxNow) in a local health clinic was analysed using a viral metagenomic approach to identify any viruses. Viral RNA was extracted using the RNA using the High Pure viral RNA kit (Roche Diagnostics, USA). The resulting RNA was used to prepare a 2x150bplibrary using the TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat.