RNA-seq of HeLa_WT_1
mRNA molecules were purified from total RNA using oligo(dT)-attached magnetic beads.Then mRMA were fragmented into small pieces followed by cDNA synthesis, End Repair, Add A, Adaptor Ligation and PCR. The library was validated on the Agilent Technologies 2100 bioanalyzer. Finally the library was amplified with phi29 to make DNA nanoball, which later were load into the patterned nanoarray and single end 50  bases reads were generated in the way of combinatorial Probe-Anchor Synthesis (cPAS).