Chromatin capture of Teleopsis dalmanni pupal cells for genome scaffolding
Intact cells from unsexed pupae from the 2A inbred line were crosslinked using a formaldehyde solution, digested using the Sau3AI restriction enzyme, and proximity-ligated with biotinylated nucleotides to create chimeric molecules composed of fragments from different regions of the genome that were physically proximal in vivo, but not necessarily genomically proximal. Molecules were pulled down with streptavidin beads and processed into an Illumina-compatible sequencing library