viral metagenomic sequencing of nasopharyngeal swab -DNA
A sample that tested positive for seasonal influenza type A and B via rapid lateral flow immunoassay (Abbot BinaxNow) in a local health clinic was analysed using a viral metagenomic approach to identify any viruses. Viral DNA was extracted using the High Pure viral nucleic acid kit (Roche Diagnostics, USA). The resulting nucleic acid was enriched for circular molecules using rolling circle amplification using the Templiphi Kit (GE Healthcare, USA). The RCA products were used to prepare 2x150bp libraries using TruSeq Nano DNA kit.