AETL_39_1
25ng of Qubit quantified genomic DNA was fragmented and adapter-tagged. Fragmented and adapter-tagged DNA was purified with HighPrep PCR beads and then amplified and indexed in a 6 cycle PCR reaction for 30 sec, cycling for 60s. The final PCR product was purified with HighPrep beads, followed by library quality control check. The Illumina-compatible sequencing library was quantified by Qubit fluorometer  and its fragment size distribution was analyzed on Agilent TapeStation.