WGS of Mumps Sheffield strain
Nucleic acid was extracted from 200 l of sample, and first and second strand cDNA synthesis was performed as previously described. Libraries were prepared using the TruSeq Nano DNA High Throughput Library Prep Kit (cat # 20015965, Illumina), with modifications as previously described. The library was sequenced on the MiSeq platform (Illumina) using a MiSeq Reagent Kit v3 (Cat #MS-1023003).