AsCas12f1 RNP pull-down and RNA purification
Purified RNA was prepared for sequencing using a TruSeq Small RNA Library Preparation kit (Illumina) according to the manufacturers instruction except an expanded size selection was performed allowing RNA species ~30-300 nts in length to be captured. After library preparation, 150 nt paired-end sequencing was performed on a MiSeq System (Illumina).