DNA-Seq of human: solution from implant
DNA Isolation from DTT solution from Orthopedic Knee and Hip implants with the FastDNA spin Kit for Soil. A PCR was approached amplify the V1V2 regions of the 16srRNA gene. In the third amplification step two indices and illumina adapters tp amplicons samples were given. Obtained products were pooled in quimolar ratios and sequences on Illumina MiSeq (2x300 bases).