RNAseq of U87MG cells : siDDX42-2 Rep3
Libraries were prepared using Qiagen QIAseq Stranded RNA Library Kits. Briefly, Total RNA is first ribodepleted. After a RNA fragmentation step, the reverse transcription step generates first strand cDNA. Then, the second strand is synthised followed by end-repair and A addition steps, allowing the ligation of an Illumina adapter. Finally, libraries were enriched by PCR.