RNA-Seq of LHCN-M2 myotube cell line mock-infected
500 ng from total RNAs were used to prepare the library with the TruSeq Stranded Total RNA Sample Preparation Kit with Ribo-Zero Gold (Illumina). To check library sizes it was used a 2100 Bioanalyzer System with the Agilent High Sensitivity DNA Kit (Agilent). Libraries were quantified by qPCR using a KAPA Library Quantification Kits for Illumina platforms (KAPA Biosystems, USA). Sequencing was performed on a NextSeq 500 System (Illumina). NextSeq 500/550 High Output v2 kit (150 cycles) were used and 2x75 bp paired-end reads were obtained.