AETL_39_2
DNA from the samples were end-repaired, cleaned up with 1x AmPure beads. Native barcode ligation was performed with NEB blunt/ TA ligase and cleaned with 0.5x AmPure beads. Qubit quantified barcode ligated DNA samples were pooled at Equimolar concentration to attain 1 g pooled sample. Adapter ligation performed for 20 minutes using NEBnext Quick Ligation Module. Library mix was cleaned up using 0.4X AmPure beads and sequencing library was eluted in 15 l of elution buffer and used for sequencing.