1P11S3_RAW
Sonication was used to randomly fragment 100 ng genomic DNA to 500 bp for each sample (Covaris S220). The End Prep Enzyme Mix was used to repair the ends of the fragments, as well as 5' Phosphorylation and dA-tailing in one reaction, followed by a T-A ligation to add adaptors to both ends. Adaptorligated DNA was then size selected, and fragments of 470 bp (with an estimated insert size of 350 bp) were recovered. Each sample was then amplified by PCR for eight cycles using P5 and P7 primers, with both primers carrying sequences which can anneal with flow cell to perform bridging PCR (BPCR) and P7 primer carrying a six-base index allowing for multiplexing. The PCR products were cleaned and verified using an Agilent 2100 Bioanalyzer, before being quantified with a Qubit 3.0 Fluorometer. Libraries with different indices were multiplexed and loaded onto an Illumina HiSeq instrument according to the manufacturers instructions and the genome was sequenced using a 2  150 paired-end sequencing kit.