RNA-Seq of Ornithodoros brasiliensis
Total RNA (1.0 g) were used to construct libraries using the TruSeq RNA sample Preparation kit (Illumina, San Diego, CA). Poly-A mRNA was isolated, fragmented (for 3 min), converted to double stranded cDNA, adapters ligated and PCR amplified for 12 cycles. Amplified bands were size selected from 450-1200 bp. Bands were excised, purified and sequenced at the Biotechnology Platform of the Agricultural Research Council (South Africa) using the Illumina MiSeq system (300 bp x 300 bp).