RNA-Seq of Bursa of Fabricius Chick
The mRNA, with poly A tails in the total RNA pools were enriched by hybridization with Oligo (dT) magnetic beads. The RNA was then sheared into fragments of approximately 309bp in length by ion interruption. Using RNA as template, the first strand cDNA was synthesized using random hexamers and reverse transcriptase, and the second strand cDNA was synthesized by using the first strand cDNA as the template. From this, the cDNA library was created. The library fragments were enriched by PCR amplification. Using the Illumina sequencing platform, the libraries were sequenced by Paired-end.