Zea nicaraguensis seedling transcriptome under 24 hour PEG stress
RNA sample was sent to BGI Shenzhen (China) on dry ice. The integrity and quantity of the RNAs were confirmed using the Agilent 2100 Bioanalyzer. Messenger RNA was then purified from the total RNA with a Dynabeads® Oligo (dT)25（Life, USA). cDNA library was constructed using NEBNext® UltraTM RNA Library Prep Kit for Illumina（NEB, USA） according to the manufacturer’s protocol ./Seeds were germinated in incubator at 28℃ in the dark. Seedlings at the two-leaf stage were transplanted into a plastic mesh grid for aquaculture and grown hydroponically with modest aeration .The nutrient solution was replaced every 3 days. The photoperiod of phytotron was 14 h of white light (illumination of 20,000 lux) at 28°C, 10 h of darkness at 22°C with a relative humidity of 70%. At the three-leaf stage, PEG6000 was added into the nutrient solution to a final concentration 16%(m/V),24 hours stressed seedlings were collected for RNA extraction.