infant gut metagenome by 454 pyrosequencing
Rapid library preparations performed using Roche GS Rapid Library Prep Kit following the Roche Rapid Library Preparation Method Manual (XL+)(May 2011). Samples were processed from emPCR through sequencing following the latest Roche emPCR Method Manuals for Paired‐End/shotgun libraries (May 2011). Libraries were run on the Roche GS FLX+ system, v2.8, flow pattern B, and analyzed through signal processing using Roche software version 2.8.