Wheat transcriptome during grain filling
RNA isolation accompanied by cDNA preparation.Illumina adapters were ligated to the cDNA molecules after end repair and the addition of an A base followed by SPRI clean-up. The resultant cDNA library was amplified using PCR for the enrichment of adapter-ligated fragments, quantified using a Nanodrop spectrophotometer (Thermo Scientific) and validated for quality with a Bioanalyzer (Agilent Technologies).