RNA-Seq of homo sapiens: adult male heart
Human tissues were homogenized using TissueLyser Disruption system (Qiagen), and total RNA was extracted using TRIzol Reagent (Invitrogen) according to the manufacturers instructions. RNA quality was assessed using denaturing agarose gel electrophoresis and spectrophotometry.  RNA was quantified using a NanoDrop1000 spectrophotometer (Thermo Fisher Scientific, UK). All analyses were performed processing only RNA samples, which displayed a 260/280-absorbance ratio 2.0. cDNA library preparation was performed by Illumina TruSeq RNA Sample Preparation Kit (Illumina, San Diego), according to sample preparation guide. Libraries were sequenced at high coverage on the Illumina HiSeq2000 NGS platform available at Genomix 4 Life (Salerno).