RNA-Seq of Drosophila melanogaster: DGRP Line 439 3wk Male Rep 1
Flies were maintained at the standard 25 C, 60-75% relative humidity, and 12-hour light-dark cycle condition on cornmeal-molasses-agar medium. At 3 weeks post eclosion, two biological replicates of 25 females or 30 males were collected for each DGRP line and stored at -80 C. Total RNA was extracted and subjected to ribosomal RNA (rRNA) depletion before being converted to a strand-specific cDNA libraries using a dUTP based protocol (Everett et al., 2020). The cDNA libraries were barcoded and randomly multiplexed into 50 pools of 16 libraries each. Each pool was sequenced on an Illumina HiSeq 2500 with 125bp single end reads.