smallRNA-seq of C. elegans: N2 wt rep1 - ife-3(xf102) control
NGS library prep was performed with NEXTflex Small RNA-Seq Kit V3 following Step A to Step G of Bioo Scientific`s standard protocol (V16.06). The amount of starting material was 500ng and the library was amplified with 15 PCR cycles can be consulted in the table below. Amplified libraries were purified by running an 8% TBE gel and size-selected for 18  40nt. Libraries were profiled in a High Sensitivity DNA on a 2100 Bioanalyzer (Agilent technologies) and quantified using the Qubit dsDNA HS Assay Kit, in a Qubit 2.0 Fluorometer (Life technologies). Samples of each individual experiment were pooled in equimolar ratio and sequenced on a NextSeq 500/550 Flowcell, SR for 1x 75 cycles plus 7 cycles for the index read.