Examination of gene expressing changes in glioblastoma cells after treatment with PIM
U87 cells were treated with 7 M  Pimavanserin tartrate or the same volume of PBS containing the equal amount of DMSO for 24 h at 37 oC with 5% CO2. Then, cells were collected (>1106), and the total RNA was extracted using TRIZOL reagent (Invitrogen) as manual instruction description. To guarantee the following experiments go smoothly, the quality of the extracted total RNA was further evaluated using Fragment Analyzer (Thermo Fisher Scientific) with Standard Sensitivity RNA Analysis Kit (15nt). Subsequently, mRNA molecules were purified with oligo(dT)-attached magnetic beads (Invitrogen Thermo Fisher Scientific, USA) and fragmented into small pieces. cDNA library was constructed with BGIEasy DNA Library Prep Kit (MGI, Shenzhen). mRNA-seq was performed on BGISEQ 500 platform (Shenzhen, china). Each sample contains three biological replicates.