Zea nicaraguensis seedling transcriptome under 24 hour waterlogging stress
RNA sample  was sent to BGI Shenzhen (China) on dry ice. The integrity and quantity of the RNAs were confirmed using the Agilent 2100 Bioanalyzer. Messenger RNA was then purified from the total RNA with a Dynabeads® Oligo (dT)25（Life, USA). cDNA library was constructed using NEBNext® UltraTM RNA Library Prep Kit for Illumina（NEB, USA） according to the manufacturer’s protocol ./Seeds were germinated in incubator at 28℃ in the dark. Seedlings at the two-leaf stage were transplanted into a plastic mesh grid for aquaculture and grown hydroponically with modest aeration .The nutrient solution was replaced every 3 days. The photoperiod of phytotron was 14 h of white light (illumination of 20,000 lux) at 28°C, 10 h of darkness at 22°C with a relative humidity of 70%. At the three-leaf stage, identical seedlings were subjected to cold stress. For the waterlogging stress, aeration was stopped and left the stems 30 mm beneath the waterline, all other conditions were kept constant.24 hours stressed seedlings were collected for RNA extraction.