metaT sequences of  surface seawater from the North East Subarctic Pacific Ocean
FASTX toolkit was used for quality control of 195.6 million 100-bp reads, imposing a minimum quality score of 20 over 80% of read length. Reads from all the libraries were aligned to an in-house rRNA database containing representative rRNAs and internal standard sequences (Gifford et al., 2011,2013) using Blastn. Reads with a bit score >= 50 were removed from the further analysis