Transcriptomic alterations in the mitochondrial ß-ketoacyl-ACP synthase-deficient mutant mtkas-2 in ambient air and in the 1% CO2 atmosphere
Experiment design: The wild-type plants were grown on Murashige and Skoog (MS) agar medium for 16 days at a temperature of 22°C, at a continuous illumination of 100 µmol of photons m-2 s-1, and in the 1% CO2 atmosphere. Three samples were collected for each combination of genotype and atmospheric condition. All MS plates were randomly placed in the growth chamber. Total RNA was extracted from 2 g fresh aerial organs using TRIzol (Life Technologies, Carlsbad, CA), and further purified using the QIAGEN RNeasy Mini Kit (QIAGEN, Valencia, CA) following the DNase I (Life Technologies, Carlsbad, CA) treatment to remove any DNA contamination. The 200 bp short-insert library was constructed. Transcriptome sequencing was performed at BGI Americas (http://bgiamericas.com) using an Illumina HiSeq 2000 system and V3 Reagent (91 pair end sequencing).