R81_hen_caecal_content
The V3-V4 regions of 16S rRNA genes were amplified with forward primer 341f and reverse primer 805r. using polymerase (Ramaciotti centre for genomics, UNSW, Sydney NSW 2052, Australia). Sequencing was performed on an an Illumina MiSeq system (2  300)bp).The upstream analysis of sequence was performed with QIIME2.