scRNA-seq of human brain organoids: 60 days in vitro
The organoids were dissociated by trypLE (Life technology) into single cells. The cell debris and other aggregates were removed by an additional low-speed centrifugation. About 5,000 cells were captured and the released RNA were barcoded through reverse transcription in individual GEMs. Droplet-based single-cell RNA sequencing was performed using the 10X Genomics Chromium Single Cell 3 platform, version 3, according to manufacturers instructions.