RNA-Seq of Mus musculus adult female thoracic spinal cord
At 8 weeks post injuri, the lesion site (4-5 mm) and the L3, L4, L5 and L6 DRGs from 4 injured Mus and 4 injured Acomys was collected and total RNA was extracted using NZY Total RNAIsolation kit (NZYtech, MB13402). Thoracic spinal cord sites and DRGs from sham animals (n=4 Mus and n=4 Acomys) were also collected to serve as controls. RNA concentration and purity was determined by NanoDrop spectrophotometry, and integrity was confirmed using Experion RNA StdSens Kit (Bio-Rad), following the manufacturer's instructions using 1uL of RNA in a quantification range 5-500 ng/ul of each purified total RNA sample. Preparation of barcoded stranded RNAseq libraries and deep uni-directional sequencing were performed at the Genomics Core Facility (GeneCore) at EMBL,Heidelberg, Germany.