gDNA, HMW from embryos from inbred colony J-1-1-1-1
flash frozen and minimally grinded embryos embedded in plugs were further digested with RNase and proteinase-K, to extract HMW gDNA. This DNA was prepared for sequencing using SQK-LSK109. Special care was needed to release the HMW DNA from the beads, with repeated elution steps. Sequencing was done on a MinION flow cell FLO-MIN106.