Transcriptome-wide analysis of antennal chemoreseptors of bumble bees at different life stages uncovered the core regulatory elements of olfaction
The high-quality RNA samples were used for both mRNA/lncRNA and small RNA library preparation using MGIEasy RNA Library Prep Set. In brief, for mRNA/lncRNA library preparation, the following steps were carried out: (i) rRNA depletion was performed to enrich mRNA/lncRNAs, (ii) reverse transcription, end repair, 3-end addition of adenine (A), adaptor ligation to RNA fragments and purifications were carried out according to kit instructions, (iii) rolling circle replication amplification, single strand separation and cyclization steps were performed then, (iv) DNA nanoball synthesis and sequencing were performed on MGISEQ-2000 (MGI, Shenzhen, China) platform at pair-end 100 bp reads.