Bacterial sequencing in Antarctic red and green snow
Approximately 100 ml of the snow melt was filtered onto 47 mm diameter sterile mixed cellulose esters membrane, and half of the membrane was cut into pieces and used for DNA extraction using the FastDNA SPIN Kit for Soil . The  V4 hyper-variable region of the bacterial and archaeal sequences were amplified using the primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACNVGGGTWTCTAAT -3')