RNA-seq of deep underground microbiome
The DNA quality in the samples was checked by agarose electrophoresis gel. Genomic DNA samples were prepared for sequencing using the Hyper kit Library, Kapa Biosystems (Roche). The quality of the libraries obtained was checked using the Fragment instrument Analyzer. Quantitative analysis was performed using qPCR method. After quality control and For estimating the amount of DNA, the pool of libraries was sequenced on an Illumina MiSeq instrument (length reads - 300 bp on both sides of the fragments). FASTQ files were obtained using Bcl2fastq v2.20 Conversion Software (Illumina).