16S rRNA Sequences (V4 region) from Cathode-Oxidizing Electrochemical Bioreactor Communities
Illumina MiSeq paired-end (2 x 250 bp) sequencing was done on the V4 hypervariable region of the 16S rRNA gene of extracted DNA by MR-DNA (Molecular Research LP, Shallowater, TX). In brief, ribosomal sequences were amplified with a 30-cycle PCR reaction with a 53C annealing temp using barcoded 515F primer paired with 806R primer, and amplicons were pooled and purified with calibrated Agencourt Ampure XP beads (Agencourt Bioscience Corporation, Beverly, MA). DNA libraries were prepared using the resultant PCR product with the Illumina TruSeq DNA library preparation protocol and sequenced on an Illumina MiSeq following manufacturers procedures (Illumina Inc., San Diego, CA).