Gastrula low pH
Larvae of the red sea urchins Mesosentrotus franciscanus at gastrula and pluteus stages, were collected under laboratory conditions that emulate current environmental variables and future scenarios of ocean acidification. Nine families (each family one male and one female) were created for each treatment (current and future). Larvae were collected at day 2 and 5. Total RNA was extracted at the USCB. Library preparation and sequencing were developed at Eurofins. In total, there were 2 pH treatments and 2 ontogenetic stages