RNAseq of Light induction of WT and SUB1 overexpression strains
Neurospora was grown in plates in light until mat layer is formed. Pieces cut from the mat were transferred to Vogel`s medium with 2% glucose and grown in light overnight. Cultures were than transferred to constant darkness for 24h. They are harvested after 0, 30, 60 and 120 min light exposure. Total RNA was extracted by Triazol reagent and mRNA libraries were prepared with NEBNext Ultra RNA Library Prep Kit for Illumina (E7530) in combination with NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490)