RNA-Seq of Entomophthora muscae sporulating virgin female housefly cadaver, early sporulation stage (4 hours post mortem)
Virgin, female houseflies were subjected to infective E. muscae conidia that was discharged from sporulating cadavers. 7 days after treatment, the flies died. 4 hours post mortem, the cadavers were collected and placed in individual Eppendorf tubes. These were snap-frozen in liquid nitrogen and stored at -80 degrees celsius. Total RNA was extracted using a modified GeneJet RNA purification kit (Thermo Scientific). RNA was sequenced using BGI Tech.