RNA-Seq of freeze-killed sporulating virgin female housefly cadaver (28 hours post mortem)
Virgin, female houseflies were freeze-killed for 6 minutes at -24 degrees celsius. 28 hours post mortem, the cadavers were collected and placed in individual Eppendorf tubes. These were snap-frozen in liquid nitrogen and stored at -80 degrees celsius. Total RNA was extracted using a modified GeneJet RNA purification kit (Thermo Scientific). RNA was sequenced using BGI Tech.