RNA-seq of urea derivative-treated Streptococcus mutans
Total RNA was isolated and rRNA was removed. First strand cDNA was synthesized and second strand cDNA was obtained with PCR. Library fragments were purified to select cDNA fragments of 400-500 bp length. DNA fragments with ligated adaptor molecules on both ends were selectively enriched. The sequencing library was then sequenced on NovaSeq 6000 platform.