wgs of Bcbva from bone samples
Genomic DNA was isolated from minimally passaged colonies grown on SBA. gDNA was isolated using the QIAGEN DNeasy UltraClean Microbial Kit (Hilden, Germany), filter sterilized through a 0.22 mm spin filter, and sterility verified according to entity specific procedures and CDC guidance. Libraries were produced with the NEBNext Ultra II Li-brary Prep kit (NEB, Ipswich, Massachusetts, USA) as described previously. Briefly, 700-800 bp inserts were size selected using magnetic beads and index barcode primers were added to fragments with minimal PCR cycles. Excess primers were removed in another round of magnetic bead size selection.