RNA-seq of drought stressed FR697 maize: Nodal root tip
Samples from field experiments selected from plants for which nodal root lengths (node 2) fell within one standard deviations of the mean for the respective genotype and treatment as well. The nodal root tip sections were pooled into six biological replicates. Each replicate contained a minimum of eight root sections representing a minimum of four plants. Root tips were frozen in liquid nitrogen and ground using a Qiagen/Retsch tissuelyser II bead-beater with 1/8" stainless steel beads from union process (part#0070-01). Root tip homogenate was then isolated using the RNeasy Plant Mini Kit (Qiagen). Isolated RNA was DNAse-treated with TURBO DNase (Thermo Fisher). DNAse-treated RNA quality was assessed using the 2100 Bioanalyzer (Agilent Technologies Inc.). RNA samples were then sent to Novogene (Sacramento, CA) for library preparation and sequencing, producing high quality paired-end 150 bp RNA-Seq libraries.