RNA-Seq of ZmEL1 non-transgenic lines: 0.5mm developing ear
Total RNA was isolated using Quick RNA Isolation Kit (Huayueyang Biotechnology CO., LTD. Beijing, China). A library with insert sizes ranging from 200 to 500bp was prepared using the commercial Illumina library preparation kits (TruSeq Stranded mRNA LT-SetA. RS-122-1201) and was sequenced following the HiSeq X-Ten protocols