RNAseq of HEK293T: GFP ectopic expression
RNAseq libraries were prepared according to the Illumina TruSeq RNA Sample Prep v2 Kit (Illumina), starting with 2 ug of total RNA and subjected to polyA-selection by oligo d(T) beads. The strand-specific dUTP library was size-selected for cDNAs corresponding to 150-225 nt and was single-end sequenced with Illumina HiSeq 2000 to produce ~101nt long fragments.