TT268t016 sheep blood transcriptome
RNA-seq of whole blood collected after an intra-mammary infection with a strain of Staphylococcus aureus at t000. RNAs were extracted for eight sampling times (post-inoculation hours). Total RNA was isolated from blood samples using the commercially available NucleoSpin RNA Blood kit (Macherey-Nagel, Dren, Germany), according to the manufacturers protocol. Obtained RNA was quantified by measuring the absorbance at 260 nm using a NanoDrop ND-1000 (NanoDrop Technologies, Wilmington, DE, USA) and integrity was checked by Fragment Analyzer (Agilent Technologies, Santa Clara, CA, USA). cDNA libraries were prepared from high quality RNA using an Illumina TruSeq RNA sample prep kit (Illumina, San Diego, CA, USA), following the manufacturers instruction. Samples were amplified by PCR and quantified by qPCR. Individual RNA-seq libraries were evaluated through a run on an Illumina MiSeq sequencer (Illumina, San Diego, CA, USA) at the GenoToul genomic platform (Castanet-Tolosan, France).