Regulatory mechanisms of metabolic flexibility in the dark-eyed junco
Juncos exposed to two by two experimental design differing in photoperiod and temperature for a duration of six weeks. At the end of the treatments, juncos were euthanized using cervical dislocation and pectoralis tissue was removed for sequencing. RNA was isolated using the Trizol method and libraries were prepared using the Illumina TruSeq Sample Prep Kit. Ten individuals were multiplexed in a single flow cell lane using Illumina index primers.