RNA-Seq of Oryza sativa leave: WT Xoc-3
Leaf samples from 8-week-old WT and OsASR6-RNAi rice plants with or without Xoc strain oxy04 inoculation at 5 dpi were collected and immediately frozen in liquid nitrogen. Total RNA was extracted using Trizol reagent following the manufacturers procedure, and checked for quality and purity by Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN value >7.0. The qualified RNA was then converted to cDNA for transcriptome sequencing performed on an Illumina HiSeq 2500 (Illumina, CA, USA) at the CapitalBio Technology (Beijing, China). The raw sequences were filtered to remove the adapter sequences and contaminated reads. The retained mappable reads were aligned with the HISAT software program using the default parameters.