cloned Pme gene
Pme gene was PCR amplified, then the purified PCR product and the vector pQE-30 was double digested with the appropriate restriction enzymes to create the sticky ends. Later the digested Pme gene and the vector were ligated to create the recombinant plasmid (pQE-30) having Pme gene inserted on it. This plasmid was then  send for sequencing.