Long-Read Genome sequencing of Streptomyces Strains from Arizona
Spores from a single colony of the strain were grown until substantial flocculation occurred in liquid media. At this point cells were pelleted and genomic DNA was extracted using a Promega Wizard kit with the addition of lysozyme. Unsheared DNA was then prepared for sequencing using a ligation based kit from Oxford Nanopore (LSK109) and sequenced on a MinION