RNA-seq of Phoronis muelleri larvae, read 2
RNA extracted from 6 larvae. mRNA purification with beads (RPBX) followed by fragmentation. First strand cDNA synthesis, second strand cDNA synthesis, 3' ends adenylation and anchor ligation was performed using commercial kits and protocols. DNA fragments were enriched by hand and pooled. qPCR was performed using the NEBNext Library Quant kit for Illumina.