Genomic DNA from Pseudococcus longispinus mealybugs
For PacBio sequencing, genomic DNA was prepared from pooled mealybugs using Qiagen Genomic Tip 500 g extraction kits, size selected for fragments >20 kb using a BluePippen device, followed by library preparation using a SMRTbell Template Prep Kit v1.0. The resulting libraries were se- quenced on 28 single molecule realtime (SMRT) PacBio cells using P6 version 2 chemistry and reagents by Sci-Life labs in Uppsala, Sweden.