Whole genome sequencing of Saccharomyces cerevisiae Spo11-111
For sub-culturing, we grew independent isolates of diploid yeast strain WYspo11 at 30 degree from single cells to colonies (about 25 divisions/passage) on solid YPD media (2% glucose, 2% peptone, 1% yeast extract, and 2% agar). 83 isolates (named as "Spo11-") were grown for 120 passages, and 10 (named as "Spo60-") were grown for 60 passages. The yeast cells were collected for genomic DNA extraction and whole genome sequencing by Illumina technology.