RNA seq of blades
Total RNA was extracted from each sample using Trizol reagent kit (Thermo Fisher Scientific, USA) and determined by NanoDrop 2000 (Thermo Scientific, USA) to measure the absorbance at wavelength of 260 nm and 280 nm, respectively. The integrity of each RNA sample was examined by 1% agarose-gel electrophoresis and by Agilent 2204 TapeStation (Agilent Technologies, Germany). Eukaryotic mRNA of each qualified sample was enriched using magnetic oligo-dT beads and fragmented randomly by RNA fragmentation buffer. First strand cDNA was synthesized by random hexamers and AMPure XP Beads (Beckman Coulter, USA) were used to purify double-stranded cDNA. RNA sequencing was performed by Illumina sequencing platform at Shanghai Applied Protein Technology Co., Ltd. following the manufacturers instructions (Illumina, USA).