MiSeq of soil bacteria: temperate agroforestry
Primer pair 341F/785R; Thermocycling conditions were as follows: initial denaturation (95C for 2 min), 3 touch-up cycles of denaturation (95C for 20 sec), annealing (50C for 30 sec), and elongation (68C for 30 sec), 25 cycles of denaturation (95C for 20 sec), annealing (58C for 30 sec), and elongation (68C for 30 sec), and final elongation (68C for 5 min). Libraries were normalized using real-time PCR followed by photo densitometry.