GSM5348402: Acne Donor 5; Homo sapiens; RNA-Seq
Patients were excluded from the study if they had been using acne medication, hormonal regulation medication, or hormonal-related implants in the past three months. Skin samples were obtained from 3mm punch biopsies in the back. Briefly, skin was digested enzymatically at 2 hours with 0.4% Dispase II at 37°C with agitation. The epidermis was separated from the dermis. Epidermis was then treated with 0.25% Trypsin and 10U/mL of DNAse I for 30 minutes. Dermis was homogenized and treated with 0.4% Collagenase II and 10U/mL DNase I for 2 hours. Isolated cells were loaded into the Chromium instrument (10X Genomics) according to manufacturer's protocol, sequenced with NovaSeqS2, and analyzed using Seurat.