GSM5004095: RAW 264.7 APOBEC1 KO Replicate 2; Mus musculus; RNA-Seq
RNA was extracted using RNeasy Mini kit (Qiagen), and treated with DNase (Turbo DNA-free kit, Invitrogen) following manufacturers' instructions. Total RNA was extracted from 10,000,000 cells in triplicate (RAW 264.7 and RAW 264.7 APOBEC1 knockout each from separate plates). RNA was extracted using RNeasy mini kit (Qiagen) and then treated with turbo DNase (Life technologies). RNA concentration and integrity was determined by a Qubit 4 (Thermo Fischer scientific) using the Qubit RNA BR assay kit or Qubit XR assay kit and the Qubit RNA IQ kit (Thermo Fischer scientific). 1μg of RNA was processed with Kapa mRNA hyperprep Kit for illumine platforms (Kapa Biosystems, Roche) and KAPA Single-Indexed Adapter Kit for Illumina® Platforms (Kapa Biosystems, Roche). Libraries were sequenced with the Illumina HiSeq 2000 v4 technology, generating 125-nucleotide, paired-end reads.