GSM4818343: ATAC-seq WT_HBVneg_rep1; Homo sapiens; ATAC-seq
Cells (5 x 104) were washed in cold PBS and lysed in 50 µl of lysis buffer (10 mM Tris HCl pH 7.5, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630). Following lysis and centrifugation, nuclei were used for transposition using Illumina Tagment DNA TDE1 Enzyme and Buffer Kit (Cat# 20034197) per manufacturer's protocol. The transposed DNA was cleaned using Qiagen Reaction Cleanup kit (Cat# 28204), eluted in 10 µL elution buffer and stored at -20oC. Transposed DNA was PCR amplified using primers containing unique adapters. Amplified library was purified using Qiagen MinElute PCR Purification Kit (Cat#28004). Pairedend 2x50 bp sequencing was performed using a HiSeq2500 system (Illumina).