GSM5195394: A_ctrl_24h_2; Homo sapiens; RNA-Seq
After infection cells were lysed with TRIzol (Life Technologies) and RNA was isolated by phenol-chloroform followed by isopropanol precipitation. Quality and integrity of total RNA was controlled on Agilent Technologies 2100 Bioanalyzer. 1 ug of RNA was used for strand-specific cDNA library construction at Novogene using a NEB directional RNA lib prep kit according to manufacturer's protocol. Libraries were sequenced on an Illumina HiSeq 4000 Platform using a paired-end 150 run. 40 M raw reads were generated from each library.