GSM5004195: dcatR rep 2; Streptomyces coelicolor; RNA-Seq
Total RNA was extracted by the modified Kirby mix method and was treated with DNase I. rRNA was removed using Ribo-Zero rRNA Removal Kit (Bacteria) (Illumina, USA) following the manufacturer's protocol. RNA-Seq library was prepared with the TruSeq Stranded mRNA Library Prep Kit (Illumina, USA) following the manufacturer's protocol.