GSM5776151: iDopa/iAstro 2A control, rep1; Homo sapiens; RNA-Seq
Triplicate samples were prepared for cLTP and control groups, with each sample comprised of 8 wells combined. Total RNA was isolated using an RNeasy mini kit (Qiagen, 74104) and treated with DNase (Qiagen 79254). Invitrogen Qubit and Agilent TapeStation were used to determine RNA concentration and RNA integrity numbers respectively prior to library preparation. PolyA RNA was isolated using the NEBNext® Poly(A) mRNA Magnetic Isolation Module and barcoded libraries were made using the NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich MA). Libraries were pooled and single end sequenced (1X75) on the Illumina NextSeq 500 using the High output V2 kit (Illumina Inc., San Diego CA).