GSM5267111: MB_2 snATAC-seq; Mus musculus; ATAC-seq
Cells were rinsed with 1X PBS and incubated with 2 mL TrypLE-Express for 5 minutes at 37C, which was then neutralized with 8 mL myoblast growth media. Nuclei were prepared following Bio-Rad's SureCell WTA 3' Nuclei Isolation protocol (https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/surecell/surecell-wta3-nuclei-demonstrated-protocol-1000000044178-00.pdf). Myotubes were both filtered (3 samples) and not filtered (2 samples) through a 40uM strainer before nuclei isolation but data were very similar between the two different nuclei preps. Tagmented DNA was barcoded and amplified in nanodroplets, then extracted following Bio Rad's SureCell ATAC-seq library kit (cat. #17004620; protocol document: https://www.bio-rad.com/webroot/web/pdf/lsr/literature/10000106678.pdf). Bio-Rad's SureCell ATAC-seq library kit (cat. #17004620; protocol document: https://www.bio-rad.com/webroot/web/pdf/lsr/literature/10000106678.pdf)