GSM5087982: Exosome, Xantho-granulomatous cholecystitis, 782875; Homo sapiens; RNA-Seq
The exosome samples were lysed using TRIzol (Invitrogen) and the RNA content was extracted according to the manufacturer's instruction. Total RNA samples (2 μg) were treated with the Epicenter Ribo-Zero rRNA Removal Kit (Illumina) and RNase R (Epicenter) to remove ribosomal and linear RNA. RNA libraries were prepared for sequencing using standard Illumina protocols