GSM5579683: Keratinocytes 3 weeks (replicate4); Mus musculus; RNA-Seq
Total RNA was isolated using RNeasy plus micro kit (Qiagen). Quantity of total RNA was assessed with the Qubit 2.0 and quality was checked using a RNA 6000 Nano chip on Agilent's bioanalyzer. Barcoded mRNA seq libraries were prepared from 140 ng total RNA using a NEBNext® Poly(A) mRNA Magnetic Isolation Module and NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® according to the manual (with a final amplification of 12 cycles) . Concentration was determined using Invitrogen's Qubit HS assay and fragment size was analyzed on Agilent's 2100 Bioanalyzer on a HS DNA chip.