GSM4877979: 5dpf_lvr_uhrf1mut_1; Danio rerio; RNA-Seq
Livers from between 20-30 larvae were dissected and put in 500 ul of Trizol. RNA was extracted the same day of the liver dissection. Total RNA was treated by DNAseI (Thermo Scientific) for 30 minutes at 37C. RNA quantitiy and quality was assesed by Qbit. 80-120ng of RNA was used for library prep. RiboZero was performed to deplete ribosomal RNA, and then used for TrueSeq Illumina library prep.