GSM5820771: sample: C99_2; Homo sapiens; RNA-Seq
Total RNA was isolated from the blood samples that contained RNA later using RiboPureTM-Blood Kit (Thermo Fisher Scientific; Waltham, Massachusetts, USA). Subsequently, GlobinLock molecular mechanism was applied to block the high globin mRNA content of erythrocytes, which is abundant in the blood and could hamper the whole blood RNA analyses Full transcriptome analysis was performed following the modified version of single-cell tagged reverse transcription (STRT) protocol, where 10 ng of high-quality input RNA was converted into cDNA and amplified to form an Illumina-compatible library. The STRTprep pipeline, available at https://github.com/shka/STRTprep/tree/v3dev, was used for processing raw sequencing reads, aligning to the hg19 genome, quantitating the expression levels.