Illumina MiSeq paired end sequencing
PCR primers: Forward: AGAGTTTGATCMTGGCTCAG, TAGAGAGTTTGATCMTGGCTCAG, CTCTAGAGTTTGATCMTGGCTCAG; Reverse: GTATTACCGCGGCTGCTG, CGTATTACCGCGGCTGCTG, TAGTATTACCGCGGCTGCTG