GSM5226785: 5637_Cis_A; Homo sapiens; RNA-Seq
RNA was extracted using the RNeasy Plus Kit (Qiagen). Cells were lysed and passed through QIAShredder column (Qiagen) according to the manufacturer's protocol. gDNA elimination columns (Qiagen) were used to remove any residual gDNA from the purified RNA. RNA library preparation was performed using the Universal Plus mRNA –Seq +UDI kit (Nugen Technologies).