GSM5579476: WT_ICM_RNA-6; Mus musculus; RNA-Seq
Freshly isolatedE.3.5 ICM, E4.5 epiblast, E4.5 primitive endoderm, E4.5 trophectoderm and E5.5 ICM and epiblast were lysed and used for RNA-seq, STAR ChIP-seq, miniATAC-seq and Brif-seq. For RNA-seq, cDNA was generated using the SMART-seq2 protocol. cDNA libraries were conduced using the Tn5 library construction protocol (Picelli et al. Nature protocols. 2014). Finally, the acquired cDNA libraries were sequenced on HiSeq X Ten platform. For STAR ChIP-seq (Zhang et al. Nature. 2016), target DNA fragments were followed by TELP library construction protocol (Peng et al. Nucleic Acids Research, 2015). Open chromatin analysis was done according to miniATAC-seq protocol (Wu et at al, Nature, 2018). Single cell methylome analysis was conducted by Brif-seq protocol (Li et at al, Molecular Plant, 2019).