GSM5226076: H6658, FTO knockdown, Input; Homo sapiens; RIP-Seq
Total RNA was extracted by Trizol reagent RNA immunoprecipitation was performed with the m6A antibody (Synaptic Syestems,202003).Both the input sample without immunoprecipitation and the m6A IP samples were used for RNA-seq library generation with NEBNext® Ultra™ RNA Library Prep Kit . Library sequencing was performed on an illumina NovaSeq 6000 instrument with 150bp paired-end reads