GSM5620726: HFF cells, Ser5P DFF ChIP-Seq Rep1 (Sample 19); Homo sapiens; ChIP-Seq
Rapid nuclei isolation steps were performed on wet ice using ice-cold buffers as previously described (Nilson et al. 2017; Ball et al. 2019). Following DFF digestion, nuclei were lightly sonicated for 20s and the soluble material was immunoprecipitated with 2.5 µg of antibodies. Library construction is detailed in the manuscript.