GSM5226879: T201_S13; Homo sapiens; RNA-Seq
Total RNA isolated with Monarch Total RNA purification kit (NEB) followed by two rounds of oligo-dT purification. SMARTer® Stranded Total RNA Sample Prep Kit - Low Input Mammalian 24 reactions, (Takara Bio); standard 8-bp i5 and i7 Illumina index barcodes and adapters were added to each library. Libraries were sequenced on the Illumina HiSeq 2500 following the manufacturer's protocols.