GSM5604869: Sample LS174T-2D-1 technical replicate h; Homo sapiens; RNA-Seq
Total RNA were extracted from the four CRC cell lines grown in either 2D or 3D conditions by using the RNeasy® Plus Micro Kit (Qiagen), as described by the supplier. Messenger (polyA+) RNAs were purified from 300 ng of total RNA using oligo(dT). Libraries were prepared using the strand specific RNA-Seq library preparation TruSeq Stranded mRNA kit (Illumina).