GSM5605397: NAFLD Patient 163; Homo sapiens; miRNA-Seq
Samples were lysed with a 1:1 ratio of HTG biofluids lysis buffer, according to manufacturer's instructions. Lysates input into nuclease protection assays run on the HTG EdgeSeq processor. Sequence adapters were added to the samples in a 16-cycle PCR reaction. PCR products were purified using AMPure XP beads (Beckman Coulter, High Wycombe, UK).