GSM5734420: MRC5-veh-C; Homo sapiens; RNA-Seq
RNA was extracted from cells using the Ambion PureLink RNA Mini Kit (Life Technologies) according to manufacturer's instructions and treated with DNase I (Amplification grade, Sigma-Aldrich). Total RNA integrity was checked using a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). RNA concentrations were measured using the NanoDrop system (Thermo Fisher Scientific, Inc., Waltham, MA). RNA libraries were prepared for sequencing using standard Illumina protocols