GSM5090093: Control sample 2; Homo sapiens; RNA-Seq
Cell pellets from 10^6 cells per sample were solved and frozen in Qiazol. RNA was extracted according to the miRNeasy mini protocol (Qiagen) followed by two steps of DNase-digestion (TURBO DNA free Kit, Ambion) Truseq-Stranded mRNA Sample Prep kit (Illumina, Inc, San Diego, CA) according to manufacturers' protocol. Molarity of each library was calculated and equal amounts were pooled and used for sequencing (10 pM).