GSM6031069: 24OH_24h_0620_2 [AB239]; Mus musculus; RNA-Seq
Messenger RNA was purified from cultured cells using Dynabeads mRNA purification kit (Invitrogen). Libraries were prepared using a modified MARS-seq protocol for bulk sequencing. Briefly, mRNA was reverse-transcribed into cDNA and amplified. cDNA was then fragmented into 300-500 kb fragments, and sample barcode and Illumina adapter sequences were added to each molecule using polymerase chain reaction (PCR). Library constracted using a modified MARS-seq technique (Keren-Shaul et al, Nature Protocols 2019). Briefly mRNA was barcoded, converted  into cDNA and amplified by PCR.