GSM5513534: RNA_seq_WT_non-pregnant_Zone1_ex1; Mus musculus; RNA-Seq
Liver samples were embedded in OCT compound and 5-µm-thick sections were prepared, mounted on polyethylene naphthalate membrane-coated slides (Leica, 11505158), dried for 5 sec at room temperature, fixed in acetone for 2 min, and then stored at -80℃. The fixed tissue sections were microdissected using a LMD7000 (Leica) and the tissue fragments were captured and collected in sampling tubes (BMBio, BMC-06) containing buffer RLT (Qiagen). Areas of 2 mm2 from each zone were subjected to RNA extract by using the RNeasy Micro Kit (Qiagen). Quality RNA samples were selected (RIN>7). 50 ng Total RNA was used to generate RNA-seq libraries using KAPA RNA HyperPrep Kit with RiboErase (Roche).