GSM4878258: G1 phase - DMSO - Replicate 1; Homo sapiens; RNA-Seq
Total RNA was isolated from two replicates of the synchronized HeLa cells using the RNeasy Plus Mini Kit (QIAGEN) as per the manufacturer's protocol. While isolating total RNA for RNA-seq analysis, on-column digestion of DNA was additionally performed using RNase-Free DNase Set (QIAGEN) as a precautionary measure and later subjected to the mRNA-seq library preparation.   RNA libraries were prepared for sequencing using standard Illumina protocols