GSM4877916: Senescent_ESCs_8d_diff_rep2; Homo sapiens; RNA-Seq
ExtractRNA reagent (Evrogen, Russia) was applied according to the manufacturer's protocol. CDNA was synthesized from total RNA using Mint-2 kit (Evrogen, Russia) according to the manufacturer's protocol. The RNA libraries were prepared with the Qiaseq FX DNA Library kit (Qiagen, Germany). The whole transcriptome RNA sequencing was performed with the HiSeq 2500 sequencing platform (Illumina, San Diego, CA, USA) in the single-end mode and with a read length of 50 bp in a rapid run mode.