Illumina NovaSeq 6000 paired end sequencing; Fly Cell Atlas: single-cell transcriptomes of the entire adult Drosophila - Smartseq2 dataset
Adult fly tissues were dissected, nuclei were extracted using dounce homogenizer, and individual nuclei were collected into 384-well plates using FACS sorter (SONY SH800), each well with one nucleus; all samples are nuclei for single-nucleus sequencing.  ERCC RNA Spike-In Mix (Thermo Fisher, 4456740) was used at a concentration of 1:600000 no nucleic acid extraction required; samples were directly processed for reverse transcription using Smart-seq2 protocol smart-seq2 protocol from Picelli et al., 2014, Nature Protocols