GSM5393380: pOD13_SuCasO_SuRepeatSpacer; Escherichia coli; RNA-Seq
RNA was extracted using Direct-zol RNA Miniprep Plus (Zymo, R2072) Ribosomal RNA was removed from the samples using RiboMinus Transcriptome Isolation Kit, bacteria (Invitrogen, K155004). 3'-phosphoryl groups were removed from RNA using T4 polynucleotide kinase (NEB, M0201S). cDNA synthesis and library preparation was performed with NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB, E7330S). Size selection for fragments between 200 bp and 700 bp was performed with Select-a-Size DNA Clean & Concentrator Kit (Zymo, D4080). DNA was cleaned up using AMPure XP beads (Beckman Coulter, A63882) and quantified with DeNovix dsDNA Broad Range Kit on DeNovix DS-11 FX (DeNovix).