GSM4726143: tongue2-A64025; Homo sapiens; RNA-Seq
Total RNA was extracted from 30 mg of fresh frozen oral cancer tissue and clinically normal contralateral gingiva or tongue tissues from oral cancer patients using the Qiagen RNeasy kit (Catalog No: 74104) following the manufacturer's protocol including the recommended DNase treatment.  Homogenized tissue lysates were prepared by using a small hand-held pestle followed by passing the lysate at least five times through a blunt 20-gauge needle fitted to an RNase-free syringe.  The RNA concentration and integrity were determined using an Agilent 2100 Bioanalyzer, equipped with an RNA 6000 Nano kit (part number 5067-1511), following the manufacturer's instructions. Ribodepleted strand specific RNA libraries were prepared from 24 total RNA samples.  Samples were pooled and 125 base paired end sequencing was performed on the Illumina 2500.