GSM5226544: RNA infected + shield_rep_1; Homo sapiens; Human betaherpesvirus 5; RNA-Seq
For RNA extraction, cells were lysed in Trizol and total RNA was prepared using Direct-zol RNA miniprep kit (Zymo Research) following the manufacturer's instructions that include an on-column DNase digestion step. RNA quality was assessed on a Bioanalyzer, and libraries were prepared using IlluminaOligo dT selection kits according the to manufacturer's instructions.  Libraries were sequenced on an Illumina NovaSEQ6000 (100bp paired-end reads). RNA-seq of polyadenylated RNA