GSM6030508: Female CD8 TILs, biological replicate 3, scRNAseq; Mus musculus; RNA-Seq
6 weeks old WT male and female C57BL/6 mice were inoculated subcutaneously with 5 x 10^5 MB49 tumor cells, which are a urothelial cell cancer cell line. On Day 10 post inoculation, single cell suspensions were prepared from the tumors after mechanical disruption and enzymatic digestion with 1 mg/mL Type IV Collagenase (Roche). Cells were strained through a 70 µM filter. Live tumor infiltrating CD8+ T cells (CD3+ CD8+ CD4-) were sorted on a BD FACSAria Ilu Cell Sorter and immediately processed for scRNA-seq. Experimental procedures for scRNA-seq followed established techniques using the Chromium Single Cell 3' Library V3 Kit (10x Genomics).  Briefly, FACS-sorted CD8+ T cells were loaded onto a 10X Genomics Chip A and emulsified with 3' Single Cell GEM beads using a Chromium™ Controller. Libraries were constructed from the barcoded cDNAs