GSM5606247: MV4;11.TDI-055.5uM.24h_B; Homo sapiens; RNA-Seq
RNeasy plus mini kit (Qiagen, #74136) was used to extract total RNA and remove genomic DNA. 1000ng total RNA was used for library preparation using polyA mRNA magnetic Isolation module (NEB, #E7490) and RNA library prep kit (NEB, #7770) following manufacturer's instructions. The library quality was assessed on an Agilent Bioanalyzer DNA chip ( Agilent, #5067-1504). RNA-seq samples were sequenced using Illumina NextSeq 500.