GSM5393473: RSA03; Homo sapiens; RNA-Seq
decidua were collected, flash frozen in liquid nitrogen, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. Total RNAs of decidua tissues were extracted using Trizol (Invitrogen, CA, USA) according to the manufacturer's instruction. Oligo(dT)-attached magnetic beads were used to purified mRNA, which then was fragmented into small pieces with fragment buffer at appropriate temperature to generate final library. The final library was amplified with phi29 to make DNA nanoball (DNB) which has more than 300 copies of one molecular, DNBs were loaded into the patterned nanoarray and single end 50 bases reads were generated on the BGIseq500 platform (BGI-Shenzhen, China).