GSM5606645: Knockout rep1 IP; Mus musculus; OTHER
Total hepatic RNA was isolated with TRIzol reagent.  Polyadenylated RNA was enriched using a Dynabeads mRNA purification kit (Sigma, # MRN10).  The mRNA was fragmented using a Bioruptor ultrasonicator (Diagenode) with 30s on/off for 30 cycles.  m6A-IP was performed using the Epimark N6-methyladenosine enrichment kit (New England Biolabs). Library preparation was performed using the Illumina TruSeq Stranded mRNA Sample Prep Kit according to previously published protocols(Dominissini et al., 2012)