GSM5514623: Rag2-/-_MC57-SIY_ISG+ DC_day 7_r3; Mus musculus; RNA-Seq
At day 7, tumors were harvested and enzymatically dissociated into a single cell suspension. Live CD103+ DC1 and ISG+ DC were FACS-sorted from the dissociated tumor cell suspension. cDNA libraries were prepared using the Clontech SMARTer Stranded Total RNAseq Kit - Pico Input (Clontech ZapR). The libraries were sequenced with an Illumina NextSeq 500.