GSM5348591: HYMIIO5; Homo sapiens; RNA-Seq
Human MII oocytes were obtained from patients undergoing in vitro fertilization and embryo transfer procedure due to a sperm quality issue or tubal obstruction. RNA-seq libraries were constructed as previously described (Liu et al., 2021). Discover-scTM WTA Kit V2 (Vazyme, N711) was used to obtain cDNA from single oocyte and GCs. The TruePrepTM DNA Library Prep Kit V2 for Illumina (Vazyme, TD503) was used for the purpose of constructing libraries. An Illumina HiSeq X platform (Shanghai, China) performs libraries sequencing. High-quality reads were aligned to Mus musculus UCSC mm9 references and FPKM value of each gene was calculated.