GSM5776242: TSC control EVs replicate 1 [TC1ev]; Macaca mulatta; miRNA-Seq
Total RNA was isolated from cells using an RNeasy kit (Qiagen, Cat #74104). Each EV sample was diluted in 5 volumes Qiazol and incubated for 5 mins at RT prior to freezing at -80°C until RNA extraction. The miRNeasy serum/plasma kit (Qiagen, Cat #217184) was used to isolate total RNA from EVs Approximately 0.4 - 1 µg of total RNA were used to prepare small RNA cDNA libraries utilizing the specified protocol for the TruSeq Small RNA Sample Prep Kit (Illumina, San Diego, USA).