GSM5087992: Oocyte, 4C (Axolotl); Ambystoma mexicanum; RNA-Seq
Oocyte was mounted to OCT medium and sliced using cryostat. RNA was isolated using TriReagent. 80 ng of total RNA was used for rRNA depletion (Lexogen RiboCop rRNA Depletion Kit). Agilent SureSelect Strand-Specific RNA Library Prep Kit was used with rRNA depleted RNA for the construction of sequencing libraries.