GSM5088037: Retina_WT_sample3; Rattus norvegicus; RNA-Seq
Lysis of the retinas was performed using a lysis buffer with (sample 4) and without detergent (sample 3) in order to capture the membrane- associated and membrane-free ribosomes. Supernatants were collected and digested with RNaseI for 45 min at room temperature with rotation. Digestion was stopped using Superase-In. The digested lysate was loaded on MicroSpin S-400 HR column and eluted by centrifugation. RNA was extracted from the flow-through using Trizol LS (Life Technologies) Libraries were prepared using the TruSeq Ribo Profile (Mammalian) Kit (Illumina, Cat# RPHMR12126), following manufacturer's instructions.