GSM6267504: P0, td, 2; Mus musculus; RNA-Seq
For scRNA-seq, DAPI-CD45-Ter119-tdTomato+ sorted cells from the fresh (without culture) bone marrows of two 4-month-old Tie2-CreERT2;Rosa26-tdTomato mice were loaded on the Chromium controller (10XGenomics) based on the number provided by the sorting equipment. The libraries were prepared using the Chromium Single Cell 3′ Reagent Kits (v3): Single Cell 3′ GEM, Library & Gel Bead Kit v3 (PN-1000075), Chromium Chip B Single Cell Kit (PN-1000073) and i7 Multiplex Kit (PN-120262) (10x Genomics) and following the Chromium Single Cell 3′ Reagent Kits (v3) User Guide (manual part no. CG000183 Rev A). Libraries were run on an Illumina HiSeq 2500 as 150-bp paired-end reads, at 20,000 read pairs per cell.