GSM5934971: WT2 spermatocytes; Mus musculus; RNA-Seq
Primary spermatocytes were collected using flow cytometry via Hoechst staining of single cell suspension of testes and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 2 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols