GSM5267700: E15.5 Scx-RNAseq control2; Mus musculus; RNA-Seq
E15.5 forelimb samples were freshly dissected,digested with trypsin-EDTA solution at 37°C for 4 minutes. After inactivation of trypsin with DMEM containing 10% FBS, cells were dissociated by pipetting. The dissociated cells were resuspended in PBS with 2% FBS and 10 mM EDTA, and filtered through a 40 μm nylon cell strainer, GFP+ cells were isolated using BD FACSAria II. RNA was extracted by Qiagen RNeasy Micro kit. Sequencing libraries were generated by using Illumina Nextera DNA Sample Prep kit.