GSM5291798: Tbx18_tdTomato 3; Mus musculus; miRNA-Seq
Tbx18+ cells were FACS sorted directly into QIAzol, and total RNA were extracted using the miRNeasy Micro Kit (Qiagen) in accordance to manufacturer's protocol. The integrity of the RNA was assessed using an Agilent Tapestation prior to library construction. RNA libraries were prepared for sequencing using the NEBNext Small RNA Library kit.