Illumina HiSeq 2500 paired end sequencing; RNA-Seq of A.thaliana (f6h1 mutant) grown in gnotobiological system with available Iron (avFe)
Roots were homogenized with Lysing Matrix E and TissueLyser II (30 beats per second for 2x30 s; Qiagen) and RNA was extracted with the miRNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. RNA quality was determined using a 2100 Bioanalyzer (Agilent Technologies).