GSM5394389: FoxM1 +/+_Rep1; Mus musculus; RNA-Seq
Tumors were harvested and dissociated into single-cell suspension. Shortly, tumors were mechanically chopped and digested with 0.1% Collagenase IV and 0.01% DNase in DMEM at 37C for 30 min. Cells were washed twice with PBS followed by lysis of RBCs with ACK Lysing Buffer (Gibco). After two washes, cells were filtered using 40 microm sterile cell strainers and trypan blue staining was performed to asses for the viability of the cells. Drop-seq was performed on the single cell suspension. Libraries were made and sequenced. The DNA sequences for both Cre and PYMT elements were added to the  mouse mm10 genome as chromosomes. Nextera XT tagmentation using Illumina cat# FC-131-1096 (Drop-seq protocol)