GSM5547956: CP-1; Mus musculus; RNA-Seq
For the quality check and counting of single cell suspension, the cell survival rate is generally above 80%. The cells that have passed the test are washed and resuspended to prepare a suitable cell concentration of 700~1200 cells/ul for 10x Genomics ChromiumTM. The system is operated on the machine. RNA libraries were prepared for sequencing with Single Cell 3′ library and Gel Bead Kit v.3 according to the manufacturer's protocol