GSM6030524: RU_25_1; Ovis aries; RNA-Seq
Total RNA were extracted using a total RNA extraction kit (Aidllab, Beijing, China) according to the instructions. RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). All the RNA samples had integrity greater than 9. RNA samples were subjected to cDNA library construction using the NEBNext UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer's recommendations.