GSM6267344: NCI-H1299 Cells, si-ZBTB11, rep 2; Homo sapiens; RNA-Seq
Trasfected NCI-H1299 cells were lysed by TRIzol® reagent, followed by RNA precipitation by isopropanol and ethanol sequentially. After air drying, the RNA pellet was redissolved in an appropriate volume of DNase/RNase-free H2O. The sequencing libraries were generated using the NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, E7530L) following the manufacturer's protocol.