GSM5087542: DNA-seq_atxr5/6 (W); Arabidopsis thaliana; OTHER
Nuclei were released by fine chopping in Galbraith's buffer and stained with PI followed by fluorescent activated nuclei sorting. DNA from 10,000 FACS sorted 16C nuclei was isolated using Pico Pure DNA Extraction Kit (KIT0103, Arcturus). Genomic DNA was sonicated to 200 bp with a Covaris S. Libraries were generated with NuGEN Ovation Ultra Low System V2 kit, according to manufacturer's instructions