GSM5393580: RS-B; Brassica napus; OTHER
Mitoribosome footprints were prepared from rapeseed flower buds as previously described (Planchard et al, 2018). Ribosome footprints were depleted from ribosomal RNA with the Ribo-Zero Plant kit (Illumina) following manufacturer's recommendations. Ribo-Seq libraries were prepared using the TruSeq Small RNA library preparation kit (Illumina). For RNA-Seq, total RNAs were extracted from 1/10th of the lysates used for Ribo-Seq analysis and treated with the Ribo-Zero rRNA Removal Kit (Illumina). One hundred ng of the rRNA-depleted RNAs were used for library construction using the NEXTflex Rapid Directional qRNA-Seq Kit (Bioo Scientific) according the manufacturer's instructions. RNA libraries were prepared for sequencing using standard Illumina protocols