GSM5225960: ESC_ATRXWT_BG4; Mus musculus; OTHER
CUT&Tag was performed as described (Kaya-Okur, H. S. et al). Briefly, nuclei were harvested and incubated with concanavalin A beads. The nuclei-beads were incubated with BG4 antibody, anti-DYKDDDK antibody, and anti-rabbit secondary antibody. The beads then incubated with pAG-Tn5 protein to pull down nuclei containing G4-Bg4 complex. The DNA was tagged adapeters by Tn5 under tagmentation. The tagged DNA was purified and PCR amplified with Nextera primers. Libraries were constructed according to the reference (Kaya-Okur, H. S. et al).