GSM5581383: islets, SMNDC1_sh3_D4; Homo sapiens; RNA-Seq
RNA was isolated using the RNeasy Micro Kit (Qiagen), including DNase treatment, following the manufacturer's instructions. Low-input full-length cDNA, was prepared with the Smart-seq2 protocol (Picelli, S. et al, Nat Protoc. 2014 Jan;9(1):171-81. doi:10.1038/nprot.2014.006) and subsequently the Nextera XT DNA library prep kit (Illumina). Library amounts were quantified using the Qubit 2.0 Fluorometric Quantitation system (Life Technologies) and the size distribution was assessed using the Experion Automated Electrophoresis System (Bio-Rad). For sequencing, libraries were pooled, diluted and sequenced on Illumina HiSeq 3000/4000 instruments using 75 bp paired-end chemistry.