GSM6030901: NPC_patient_1; Homo sapiens; RNA-Seq
Visium Spatial Gene Expression Slide & Reagents Kit The frozen tissues were placed onto Visium spatial gene expression slide (10× Genomics), fixed and permeabilized to release RNA which bond to adjacent capture probes. Next, RNA reverse transcription, cDNA synthesis, denaturation and amplification, quality control as well as spatial gene library construction were performed using Visium spatial gene expression reagents kit (10× Genomics). The spatial transcriptome library was sequenced on a NovaSeq 6000 (llumina), and mapped to the human genome (hg38) using Spatial Ranger (v 1.3.1, 10× Genomics).