GSM5513451: SVZ3; Mus musculus; RNA-Seq
RNA from the normal brain, tumor tissues, and YAP-NSC cells were extracted using TRIzol (Life Technologies), followed by purification using a RNeasy Mini Kit (Qiagen). Illumina TruSeq RNA Sample Prep Kit was used with 1 ug of total RNA for the construction of sequencing libraries. Dissociated tissues were fixed for 15 min at room temperature with 1% formaldehyde-containing medium. Nuclei were isolated and sonicated in sonication buffer (10 mM Tris-HCl pH 8.0, 1 mM EDTA, 0.5 mM EGTA and protease inhibitor cocktail). Sonicated chromatin (~300 μg) was used for immunoprecipitation by incubation with appropriate antibodies (4 μg) overnight at 4℃. RNA-seq libraries were prepared using Illumina RNA-Seq Preparation Kit. ChIP-seq libraries were prepared using NEBNext ChIP-seq Library Prep Master Mix Set for Illumina (NEB catalogue number E6240L)