GSM4727180: QUAD_3_KO_B6;129; Mus musculus; RNA-Seq
Total RNA was isolated from snap-frozen quadriceps muscle and spinal cord paired samples (from the same animal), preserved at -80C. Samples were homogenized in Trizol and total RNA was purified on-column using the PureLinkTM RNA Mini Kit (Invitrogen, Waltham, MA) according to manufacturer's protocol and including an on-column DNase digest (PureLink™ DNase Set DNAse set, Invitrogen, Waltham, MA).  The quality of the extracted RNA was evaluated using the Agilent 2100 Bioanalyzer and the Eukaryote Total RNA Nano chip (Agilent, Santa Clara, CA). cDNA libraries were prepared for sequencing using NEBNext Ultra RNA Library preparation kit (New England Biolabs). Libraries were sequenced (single-end mode and length 61 bp) on the Illumina HiSeq2500 system.