GSM5855918: Prmt5-CKO EAE SP scRNA-seq; Mus musculus; RNA-Seq
Single-cell suspensions were harvested from fresh tissues according to standard procedures. For single-cell RNA-seq, single-cell gel beads in emulsion (GEMs) were generated on a GemCode Single Cell Instrument (10x Genomics). Single-cell RNA-seq cDNA libraries were prepared using Chromium™ Single Cell 3' V3.1 Reagent Kits (10x Genomics) according to manufacturer's protocol. For single-cell RNA-seq, RNA libraries were generated by 10xGenomics Single Cell 3' V3.1 Library Gel Bead Kit and prepared for sequencing using standard Illumina protocols