GSM5394492: si_7-6 days – 2; Mus musculus; RNA-Seq
1 μg of total RNA was subjected to library synthesis using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina.1 μg of total RNA was enriched for the Poly-A mRNA and reverse transcribed to double-stranded cDNA. The cDNA was end repaired, adenylated, and ligated with Neb indexes prior to 12 cycles of PCR. Library was prepared with a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB 7760, New England Biolabs, Ipswich, MA, USA), according to the manufacturer's protocol.