GSM6267383: Castration-resistant prostate cancer [CRPC #1]; Homo sapiens; RNA-Seq
Tumor tissues were ressected by biopsy surgery and transported in DMEM/F12 medium on ice to the laboratory within an hour. For scRNA-seq, the tissues were dissociated into single cells suspensions using digestion buffer (DMEM/F12 Medium+ 2 mg/ml of collagenase IV + 100 IU/ml DNase I), and single cell GEMs were generated by 10X genomics Chromium Controller. cDNA library was constructed following the manufacture's protocol. The cell suspension of each sample was run in the Chromium Controller with appropriate reagents to generate single cell gel bead-in-emulsions (GEMs) for sample and cell barcoding. The libraries were then pooled and sequenced on a NovaSeq 6000 (Illumina) at a depth of ~400 M reads per sample.