GSM4725936: dm-ter-screen-gRNA-m2-1: Embryonic lethal CRISPR-KO screen teratoma 2 10X replicate 1 gRNA reads; Homo sapiens; RNA-Seq
Teratomas were excised and dissociated using the GentleMACS tuomor dissociation protocol, resuspended in PBS-BSA and loaded onto the 10X Genomics Chromium system as per manufacturer's instructions Single cell libraries were constructed with the Single Cell 3' Reagent Kit from 10X genomics