GSM6322735: S3, post-MSCT 6 months; Homo sapiens; ssRNA-seq
Peripheral blood samples were sequentially obtained pre-MSCs transplantation, 3 days and 6 months post-transplantation. Approximately 10 ml of blood per sample was collected into EDTA coated tubes for isolation of PBMCs. Total RNAs were extracted with Qiagen RNeasy kit. Strand-specific RNA-seq libraries were prepared using NEBNext Ultra Directional RNA Library Prep Kit for Illumina and sequenced on Hiseq 3000 sequencer platform with 150 bp paired-end reads. strand-specific RNA-seq library