GSM5393699: Zcchc8_KO_3_3Pseq_EPAP; Mus musculus; RNA-Seq
Total RNA for 3P-seq was isolated by Trizol extraction using standard procedures 3PS samples were either mock treated or incbated with E.coli Poly(A) polymerase (EPAP). rRNA was depleted using the RiboCop rRNA Depletion Kit (Lexogen). Libraries for QuantSeq 3'mRNA Seq FW + UMI were prepared by Lexogen and included the addition of spike-ins ERCC Mix1 and SIRV E0