GSM5194357: NB in control media #1; Homo sapiens; RNA-Seq
Total RNA was extracted with RNeasy PLUS mini kit (Qiagen) and the quality was checked with a Bioanalyzer 2100 (Agilent, RIN >8.0). RNAseq libraries were prepared with 50 ng of total RNA with SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara, Clontech). Samples were sequenced in whole RNAseq using the NextSeq500 Illumina Platform (75 bp single read).