GSM5732693: prp4k3-small RNA seq_R1-repeat3; Arabidopsis thaliana; miRNA-Seq
Total RNA was extracted using Trizol reagent from leaves of three week-old plants.RNA-seq library was prepared with TruSeq Stranded Total RNA kit (Illumina) according to the manufacturer's manual. small RNA-seq libarary was prepared according to D. Sun, Z. Ma, J. Zhu, X. Zhang, in Arabidopsis Protocols. (Springer, 2021), pp. 225-254.. RNA libraries were prepared for sequencing using standard Illumina protocols