GSM4726026: E12 ovary XX Rep1 [170724_O]; Mus musculus; RNA-Seq
Tissues were enzymatically dissociated. Viable single cells, from all samples except E12.5 ovaries and testis, were enriched by FACSorting. 3000 to 7000 single cells were loaded on a 10x Chromium instrument (10x Genomics). Single-cell RNA-Seq libraries were prepared using the Chromium Single Cell 3′ v2 Reagent Kit (10x Genomics) according to manufacturer's protocol.