GSM5268058: flag-hct116-notf-down; Homo sapiens; OTHER
The iCLIP protocol was followed to isolate RNA bound to ORC1-3xFlag and amplify a cDNA library as described in Mas et al. 2021. Adapter ligation was performed after immunoprecipitation and samples for each condition were barcoded during RT, using RT primers with specific barcodes. Random nucleotides (UMIs) were also incorporated into cDNAs to distinguish between PCR duplication of cDNA products.