GSM6321961: untreated wt rep3; Yarrowia lipolytica; RNA-Seq
The RNA was extracted using TRI Reagent(Thermo Fisher Scientific, Waltham, MA, USA). RNA concentration and integrity were measured by a NanoDropTM 2000 Spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), respectively. The qualified total RNA was used for cDNA library construction with a NEBNext Ultra II Directional RNA-Seq kit (NEB, Ipswich, MA, USA) and sequenced under an Illumina NovaSeq 6000 platform by ebiogen Inc. (Seoul, Korea).