GSM5089706: H522 shIWS1/MT-R Rep 2; Homo sapiens; RNA-Seq
For the RNA extraction, total cell RNA was extracted using the PureLink RNA Kit (Invitrogen, Cat. No 12183018A) For the RNA-seq, RNA samples were analyzed on Advanced Analytical Fragment Analyzer, using an RNA kit for integrity check and quantification. 100 – 500 ng of total RNA from each sample used as input for library preparation using the Illumina TruSeq stranded mRNA Library Preparation Kit (Cat. No. RS-122-2101) and they were individually indexed. Resulting libraries were quantified on Fragment Analyzer using a next generation sequencing (NGS) kit and the libraries of all the samples were pooled in equal molar concentration.