GSM5088685: ΔH3.3B; ΔH3.3A round 2 sample5; Drosophila melanogaster; RNA-Seq
For each sample, five male third instar larvae brains were homogenized and RNA was purified using the Directzol microprep kit (Zymo research) and the standard protocol with on column DNase treatment was used. Total-RNA libraries were synthesised using the Ovation RNA-Seq system for Drosophila (NuGEN). Standard protocol procedure with integrated DNase treatment was used to make the sequencing libraries. Fragmentation was performed using a Covaris E220 Focused-ultrasonicator with the recommended settings for 200 base pair target length.