GSM5820824: IPAH 1; Homo sapiens; RNA-Seq
RNA was isolated from ex vivo isolated donor and PAH PAAFs from early passage using the miRNeasy mini kit (Qiagen) combined with on-column DNase digestion (DNase-Free DNase Set, Qiagen) to avoid contamination by genomic DNA. 2µg total RNA was used as input for TruSeq Stranded mRNA Library preparation following the low sample protocol (Illumina).