GSM5194429: snRNA-seq plate #7; Arabidopsis thaliana; RNA-Seq
Each nucleus passing both DAPI and GFP gating was collected in 4 μL of cell lysis buffer (Picelli et al., 2014a) supplemented with 25 mM dithiothreitol (DTT) in single wells of 96-well plates. Smart-seq2 libraries were prepared following the published SmartSeq2 single-cell protocol (Picelli et al., 2014a; Picelli et al., 2014b) with an additional 30-minute 37°C incubation before reverse transcription. single-nucleus mRNA-seq (snRNA-seq)