GSM5227185: Sample 3 Prostate Tumor; Homo sapiens; ATAC-seq
Nuclei Isolation Buffer (NIB) was used to extract nuclei from flash-frozen prostate tumors via dounce homogenizer. Nuclei were tagmented with specific barcode sequences annealed to the Tn5 enzyme after being sorted to a 96-well plate. Tagmented nuclei were mixed and sorted to another 96-well plate. A second set of indices were introduced via PCR to generate sci-ATAC-seq libraries. Clean-up was done using Qiagen PCR purification kits, followed up by AMPure bead purification twice. single-cell ATAC-seq