Illumina MiSeq paired end sequencing
PCR primers: Forward: GYGCASCAGKCGMGAAW, CGYGCASCAGKCGMGAAW, TRRTGYGCASCAGKCGMGAAW, ACAATTWGYGCASCAGKCGMGAAW; Reverse: GGACTACVSGGGTATCTAAT, TCGGACTACVSGGGTATCTAAT, AACAGGACTACVSGGGTATCTAAT, CTTTCTGTGGACTACVSGGGTATCTAAT