GSM5460705: placenta, FET02; Mus musculus; RNA-Seq
The total RNA of mouse placenta tissue was collected according to the method described in the instructions of Trizol reagent (Invitrogen, USA), and identified and quantified using Agilent 2100 bioanalyzer (ThermoFisher Science, USA). After a qualified specific RNA library was established, it was sequenced on the computer. SOAPnuke (v1.5.2) was used to filter the sequencing data, HISAT2 (v2.0.4) was used to map the filtered high-quality reads with the reference gene set database established by BGI, and Bowtie2 (v2.2.5) was used for lncRNA and mRNA identification. Then RSEM (v1.2.12) was used to standardize the expression of lncRNA and mRNA with fragments per kilobase per million (Fragments Per Kilobase per Million, FPKM). RNA libraries were prepared for sequencing using standard lllumina protoclos