Illumina HiSeq 4000 sequencing; Single cell RNA-seq profiling of T cells isolated from LNs of MC38 tumor-bearing mice
Draining LNs from mice with MC38 tumors (~190 mm3) were excised and single cell suspensions were stained and sorted for CD45+TCRβ+T cells.  Sorted cells were loaded onto a 10x Chromium Chip using reagents from the Chromium Single Cell 5' Library and Gel Bead Kit (10x Genomics) according to the manufacturer's protocol.  Amplified cDNA 11was used for both 5' RNA-seq library generation and TCR V(D)J targeted enrichment using the Chromium Single Cell V(D)J Enrichment Kit for Mouse T Cells (10xGenomics). 5' RNA-seq and TCR V(D)J libraries were prepared following the manufacturer's user guide (10x Genomics).