GSM4998327: CD140; Mus musculus; Bisulfite-Seq
After extracting the sample DNA, DNA concentration and integrity were detected by electrophoresis. After passing the detection, the database was built. The DNA was randomly interrupted to about 350BP by ultrasound. After terminal repair, base A was added, and sequencing joint was added, the unmethylated C in the disulfite DNA fragment was converted into U, and then fragment screening and PCR amplification were performed to complete the preparation of DNA methylated library. After the library quality control, the library can be sequenced.