GSM5578549: BLM EVs, rep1 [B1]; Mus musculus; miRNA-Seq
Primary alveolar macrophages were isolated from PBS or BLM mice. After incubation at 37°C for 72 h, the supernatants were harvested and AMs-derived EVs were separated by unltracentrifugation. EVs were flash frozen on dry ice, and totla RNA was harvested using Trizol reagent. TruSeq Rapid SR Cluster Kit (#GD-402-4001, Illumina) (Illumina) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols