GSM5196009: heart, Non-transgenic control; Mus musculus; RNA-Seq
Isolated hearts from NTG and TG mice were homogenized in 1 mL of TriZol (Bio-Rad,  Hercules, CA) using a bead homogenizer, and total RNA was extracted using the Aurum Total  RNA Fatty and Fibrous Tissue Kit (Cat. No. 732-6820, Bio-Rad Laboratories, Hercules, CA),  according to the manufacturer's instructions. mRNA in the total RNA  was translated into a library of template molecules of known strand origin using the TruSeq®  Stranded mRNA Library Prep kit (Cat. No. RS-122-2201, Illumina, Inc., San Diego, CA). Poly-T  oligos attached to magnetic beads were used to isolate the mRNA, which was then fragmented  and reverse transcribed. The resultant cDNA products were amplified, and cDNA libraries ready  for sequencing were created using sequencing adapters and barcodes ligated onto the cDNA  fragments for each respective sample.