GSM5856006: bamRNAi_hs-bam_polysome_1; Drosophila melanogaster; RNA-Seq
RNA was isolated using TRIzol (Invitrogen, 15596026) (for input samples) or phenol-chloroform treatment (for polysome samples) , treated with DNase (TURBO DNA-free Kit, Life Technologies, AM1907). To generate mRNA-enriched libraries total RNA was treated with poly(A)tail selection beads. Following the manufacturer's instructions of the NEXTflex Rapid Directional RNAseq Kit (Bioo Scientific Corp, NOVA-5138-08), except RNA was fragmented for 13 minutes