GSM5394210: NGP_DMSO_rep1; Homo sapiens; RNA-Seq
Total RNA was extracted from live cells using Trizol reagent and purified by Direct-zol RNA Purification Kit. RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system. The mRNA enrichment, mRNA fragmentation, second-strand cDNA synthesis, size selection, PCR amplification, and sequencing were performed by BGI using DNBseq sequencing technology.