GSM6165243: Patient 2 [post_2B]; Homo sapiens; RNA-Seq
Total RNA of 1-1.5x10^6 MSC per culture was extracted using TRIzol reagent (Invitrogen) according to manufacturer´s instructions. cDNA was synthesized and amplified with the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio). After amplification the samples were cleaned using magnetic beads. Sequencing was performed (Novogene) and16.8 million reads were obtained per samples with single/paired end sequencing.