GSM5090151: LNCaP_dox-3; Homo sapiens; RNA-Seq
RNA was isolated by the RNeasy Mini Kit (Qiagen). DNA was removed by treating with DNase from the kit (Qiagen). cDNA was generated using All-In-One RT MasterMix (ABM, G490). qRT-PCR was performed with 1x SYBRTM Green PCR Master Mix (Thermo, 4309155) using primers that had been optimized for their melt curve. Libraries were generated using Illumina's TruSeq kit