GSM5580104: S2_OsTir1_Auxin_1_STARRseq; Drosophila melanogaster; OTHER
After 24 hours, cells were harvested and total RNA isolated using the Qiagen Rneasy MaxiPrep. A nested PCR strategy on the reporter cDNA was used used to prepare libraries as previously described (Neumayr et al 2019 Current Protocols).