GSM4726740: CAR 1_A; Homo sapiens; RNA-Seq
T cells were resuspended in 250ul PBS and placed in 750ul TRIzol LS (Invitrogen). RNA was extracted using the RNeasy Mini Kit (QIAGEN) according to the manufacturer's protocol. Total RNA integrity was checked using the Bioptic Qsep100 (BIOptic) and quantified using a Synergy H1 (BioTek). RNA libraries were prepared for sequencing using standard Illumina protocols