GSM5184268: Sertoli Hi-C rep2 (P7); Mus musculus; Hi-C
Hi-C were performed for each cell type on  about 1 million primary Sertoli cells using the Arima Hi-C Kit (#A510008).  Cells were fixed in 2% methanol-free formaldehyde in PBS at RT for ten min. with occasional mixing then quenched by addition of glycine to 0.125M with occasional mixing for 5 min.. Cells were placed on ice for 15 min. prior to washing with 1X PBS and stored at -80o C.  Hi-C was performed according to Arima Genomics (Document part # A160134 v00).  Sequencing libraries were prepared using the KAPA Hyper Prep Kit and xGen Duplex Seq adapters (Integrated DNA Techologies) according to Arima Genomics (Document part # A160139 v00).