GSM5934869: H2O vehicle treated rep1; Homo sapiens; RNA-Seq
Total RNA was extracted from cells post- treatment using RNeasy® mini kits (Qiagen, Melbourne, Australia), then checked for quality and quantity using an Agilent 2100 Bioanalyzer (Agilent Technologies, Melbourne, Australia) and NanoDrop 1000 (Thermo Fisher Scientific), respectively. RNA-seq libraries were prepared for all samples from 1 µg total RNA using a TruSeq RNA Sample Preparation Kit (Illumina, Victoria, Australia) as per the manufacturer's instructions.