GSM5001901: ML3289 (OAY470 LEU2::-gal1/10-GapR-3xFLAG) in raffinose, alpha-factor arrested, and induced with galactose, replicate 1; Saccharomyces cerevisiae; ChIP-Seq
ChIP was performed as previously reported (Guo et al, 2018). Libraries were constructed by end repairing DNA with T4 DNA polymerase (NEB), T4 PNK (NEB), and Klenow large fragment (NEB). 3' overhangs were added with Klenow (3'→5' exo-) (NEB). Y-shaped Illumina adapters were ligated onto the DNA, and libraries were amplified with Kapa HiFi Polymerase (Kapa Biosystems). Libraries were size selected to be 200-500 bp.