GSM5514402: ChIP DNA - TH2A; Mus musculus; ChIP-Seq
The protocol described by Hisano et al., 2013 was followed for ChIP in mice sperm with slight modifications (Hisano et al., 2013). TH2A and TH2B library preparation was done and the quality assessment was performed on automated electrophoresis system using TapeStation Analysis Software A.02.02 (SR1) (Agilent). Sequencing was performed with Illumina Hiseq using 150 bp paired end sequencing. For Input and TH2B ChIP‐Seq libraries, ~ 30 million and for TH2A 34 million unique paired end reads were attained. The fastq file were checked using FastQC. Based on quality report of fastq, files were trimmed using Trimmomatic (Ver-0.36) and were processed further.