GSM5513471: wt 9we rep3; Mus musculus; RNA-Seq
Tissue was lysed in TRI reagent (Molecular Research Center, Inc., Cincinnati, OH, USA) and RNA extracted with Direct-zol RNA MiniPrep kit including Zymo-Spin II and DNAse restriction step (R2050, Zymo research, Irvine, CA, USA). Library preparation was performed, starting from 200ng total RNA, using the TruSeq Stranded mRNA Library Kit (#20020595, Illumina, San Diego, CA, USA) and the TruSeq RNA UD Indexes (#20022371, Illumina). 15 cycles of PCR were performed.