GSM5087801: Gpr126_WT_4; Mus musculus; RNA-Seq
Total RNA was extracted from 4 pools of Gpr126+/+ (WT) and 5 pools of Gpr126Δ7/Δ7 (KO) mouse embryonic ventricles (4 ventricles per pool) at E12.5. The tissue was homogenized in TRI ReagentTM Solution (Invitrogen, AM9738) using steel beads in the TissueLyser LT Adapter for 5 minutes at 50 Hz. For RNA extraction, the PicoPureTM RNA Isolation kit (Applied Biosystems, KIT0204) was used according to the manufacturer's instructions. RNA was quantified and its purity checked with a NanoDrop ND-1000 spectrophotometer (Thermo Scientific). RNA integrity was verified with an Agilent 2100 Bioanalyzer instrument (Agilent Technologies). cDNA libraries were prepared with the NEBNext Ultra RNA Library Prep Kit (New England Biolabs). Libraries were sequenced on a HiSeq 2500 system (Illumina) to generate 60 base single reads.