GSM6165076: HCT116 cells, siBRD8-6, replicate 2; Homo sapiens; RNA-Seq
RNA was extracted from the cells using RNeasy Plus Mini kit (Qiagen). 100 ng of total RNA was used for the construction of sequencing libraries. RNA-seq libraries were prepared using an NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) according to manufacturer's instructions.