GSM5460321: STR_9_WT_8wk; Mus musculus; RNA-Seq
Single nuclei were isolated from ½ hemisphere striatal or cortex in Nuclei EZ Lysis buffer (Cat#NUC101-1KT, Sigma-Aldrich) and incubated for 5 min. Samples were passed through a 70μm filter and incubated in additional lysis buffer for 5 min and centrifuged at 500 g for 5 min at 4°C before two washes in Nuclei Wash and   Resuspension buffer (1xPBS, 1% BSA, 0.2U/μl RNase inhibitor). Nuclei were FACS sorted using DAPI to further isolate single nuclei and remove additional cellular debris. These nuclei were run on the 10x Chromium Single cell 3' gene expression v3 platform. RNA libraries were prepared for sequencing using 10x Genomics single cell v3 and standard Illumina protocols