GSM5001809: TCR-Lung-MB-SIIN; Mus musculus; OTHER
Tumor-bearing lung tissue was collected 5 weeks post-tumor initiation from ten KP LucOS mice. Single cell suspensions were labeled with TotalSeq-C hashtag antibodies and FACS sorted for CD44+ CD8α+ SIINFEKL+ and CD44+ CD8α+ SIYRYGGL+ T cells. 10X Genomics Chromium Next GEM Single Cell 5′ Library and Gel Bead Kit v1.1; 10X Genomics Chromium Single Cell V(D)J Enrichment Kit, Mouse T Cell. Gene expression libraries for SIINFEKL and SIYRYYGL were sequenced individually using HiSeq X (Illumina). VDJ and hashtag barcode libraries were sequenced with HiSeq 2500 (Illumina). 