GSM4818924: ciCPCs at Passage 3 (replicate 2); Mus musculus; RNA-Seq
Total RNA was prepared using the RNeasy Plus Mini Kit with Qiashredder columns  (Qiagen). Potentially residual DNA was removed by on-column DNase digestion with  RNase-free DNase (Qiagen). Total RNA was quantified on Nanodrop and quality was checked by electropherogram. Total RNA (10 μg) was used as input material for  preparing the RNA sequencing (RNA-seq) libraries following the standard Illumina RNASeq  protocol as previously described (Wamstad et al., 2012)