GSM5514524: MD1369; Homo sapiens; RNA-Seq
Muscle tissue was collected, snap-frozen in liquid nitrogen and stored at −80°C until analysis. Total RNA from calf muscle tissues was isolated using Tri Reagent (Sigma #93289) RNA-Seq analysis was performed using Novogene in-house protocol on the Illumina NovaSeq platform (HWI-ST1276) with paired-end 150bp sequencing strategy RNA library is formed by polyA capture and reverse transcription of cDNA.