GSM6323442: scRNA-WT2; Mus musculus; RNA-Seq
After obtaining the retinal tissue from mouse eye dissection, the Papain Dissociation System (Worthington, Columbus, OH, USA) was used to dissociate the tissues into a single-cell suspension. ). Briefly, the retinal tissue was placed in a mixture of 100 µl papain solution and 5 µl of DNase solution. The mixture was incubated at 37 °C for 15 min after gentle titration. The dissociated cell solution was then diluted with 900 µl of PBS and passed through a 35 µm cell filter. Dissociated samples were loaded onto the 10X Chromium system, and single-cell RNA-seq libraries were prepared using the Chromium Single Cell 3' reagent kit v3. Following the manufacturer's protocol, single cells were captured in nanolitre-scale Gel Beads-in-emulsion (10X Genomics), where cells were lysed and cDNA was synthesized by reverse transcription of RNA, and all cells were tagged with the same 10X barcode. Following PCR amplification, enzymatic fragmentation, adaptor attachment, and sample indexing, the constructed library was then sequenced using the NextSeq 500 system (Illumina).