GSM6030647: BCP rep3; Rattus norvegicus; miRNA-Seq
The lumbar enlargement spinal cord was quickly isolated out, removed the meninges, and dissected longitudinally on ice; then the ipsilateral spinal cord was snap-frozen and preserved in liquid nitrogen.Total RNA was extracted from the lumbar spinal cord tissue using a RNeasy Mini Kit (Cat# 74106,  Qiagen)  according to manufacturer instructions and the quality was assessed by Agilent 2100 Bioanalyzer (Agilent technologies Santa Clara,US). RNA libraries were prepared for sequencing using standard Illumina protocols