GSM6161676: D6_ASD; Homo sapiens; RNA-Seq
The single cell organoid cell suspension was resuspended in PBS with 0.05% BSA. Dissociated single cells for Single-cell RNA Sequencing were stained for viability assessment using Calcein-AM (Thermo Fisher Scientific, Waltham, MA, USA) and Draq7 (BD Biosciences, New Jersey, USA). Cells (2000-10,000) were loaded on a 10x Genomics Chromium instrument to generate single-cell GEMs. scRNA-Seq libraries were prepared according to the manufacturer's instructions.