GSM5089957: NFATc2 rep1; Homo sapiens; RNA-Seq
Cells were scraped off from the surface in trypsin-versene solution and collected by 500 g centrifugation. The pellet was washed with PBS to remove residual media. Total RNA extractions were performed with the RNA-Quick Purification Kit following the manufacturer's protocol. The RNA concentrations were determined using a Nanodrop ND1000 spectrophotometer . The mRNA sequencing libraries were performed with the VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina (NR604-01, Vazyme Biotech, Nanjing, China), VAHTS RNA Multiplex Oligos Set1- Set2 for Illumina (N323, Vazyme Biotech, Nanjing, China), VAHTS DNA Clean Beads (N311-01, Vazyme Biotech, Nanjing, China), VAHTS mRNA Capture Beads (N401-01, Vazyme Biotech, Nanjing, China). The quality assessments were performed in the laboratory of GENEWIZ, lnc (Suzhou, China).