GSM5087491: PRWT_WT2; Mus musculus; ATAC-seq
Fresh cells were cytoplasmically lysed then tagmentation using Tn5 enzyme was performed. Tagmented DNA was purified, then library was amplified and repurified. DNA fragments between 200-600bp in size were selected using magnetic beads. Libraries were quantified and sequenced on Illumina HiSeq 2500 (150bp read, paired-end).