GSM5776482: Ker-CT-SCR_rep1; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNeasy mini kit (Qiagen) and purified from DNA contamination through a DNase I (Qiagen, IT) digestion step. Quantity and integrity of the extracted RNA were assessed by NanoDrop Spectrophotometer (NanoDrop Technologies, DE) and by Agilent 2100 Bioanalyzer (Agilent Technologies, CA), respectively. RNA libraries for sequencing were generated in triplicate using 400ng of RNA for each sample according to the Illumina TruSeq Stranded Total RNA kit with an initial ribosomal depletion step using Ribo Zero Gold (Illumina, CA).