GSM5195919: EE501F2_093_C_T2_R2; Solanum chacoense; RNA-Seq
RNA was isolated using the Qiagen RNeasy Plant Mini Kit (Qiagen, Hilden, Germany). Samples were Turbo DNase (Thermo Fisher Scientific, Waltham, MA, USA) treated, and RNA quantity and quality was assessed using an Agilent 4200 TapeStation (Agilent Technologies Inc., Santa Clara, CA). Libraries were prepared using the Illumina TruSeq Stranded mRNA Library Preparation Kit and approximately 30,000,000 50nt single-end Illumina reads were generated for each sample on the Illumina HiSeq 4000 platform.