GSM5291676: PBAT wildtype blastocyst; Mus musculus; Bisulfite-Seq
Cells were lyzed by proteinase K and 2% SDS. Cell extract was directly applied to library construction. Libraries were constructed using the post-bisulfite adapter tagging method (Miura et al., 2012; Au Yeung et al., 2019). Pooled  blastocysts were spiked with 1% unmethylated lambda phage DNA (Promega). Libraries were amplified with KAPA library amplification kit (KAPA) for 4 cycles using primers AATGATACGGCGACCACCGAGATCTACAC and CAAGCAGAAGACGGCATACGAGAT. Libraries were sequenced on HiSeq 1500 and NovaSeq 6000 (Illumina) (HiSeq: HCS v2.2.68 and RTA v1.18.66.3; NovaSeq: NVCS v1.6.0 and RTA v3.4.4) using TruSeq SBS kit v3-HS and NovaSeq S1 reagent kit v1 (Illumina) according to manufacturer's protocols