GSM5732666: ATAC_2h1_rep2; Arabidopsis thaliana; ATAC-seq
200 cotyledons were dissected and 20.000 4C nuclei were isolated upon centrifugation over a Percoll gradient and fluorescence activated nuclear sorting (FANS) before flash freezing in liquid nitrogen. Tagmentation and following steps of ATAC-seq were performed on intact nuclei using a Nextera DNA Library Preparation kit (FC-121-1030) following the instructions of the manufacturer. Nextera DNA library prep Kit Illumina Cat #FC-121-1030