GSM5598098: THP-1 CTL ribo; Homo sapiens; OTHER
Total RNA was isolated from control cells or YTHDF1 knockdown THP-1 cells using TRIzol reagent. m6A-antibody was used for capturing m6A-modified mRNA. Ultracentrifugation was used for collecting ribosome-protected mRNA fragments. 1 ug of total RNA was used for the construction of sequencing libraries accroding to TruSeq Small RNA Sample Preparation Kit (Illumina).