GSM5090050: Tbx5G125R_Atria_PCM1+_2; Mus musculus; ATAC-seq
Isolated nuclei from both atria pooled together per individual were stained for PCM1 (pericentreolar material 1). Samples were FACS sorted and gated in such a way that debris, nuclei clumps, and nuclei with no cardiomyocyte origin were excluded. Nuclei were collected and successively washed with PBS and used as input for ATAC-seq using Illumina Tagment DNA enzyme and buffer (20034197) according to protocol. Libraries were sequenced paired end 150bp on an Illumina Hiseq4000.