GSM6030603: MCF7, AROE, ENZ, ER; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 20 minutes. After quenching and cell lysis, chromatin was sonicated using a E220 focused-ultrasonicator (Covaris). ChIP-seq libraries were prepared using the TruSeq ChIP Library Preparation Kit. 10 ng of DNA was used as starting material for input and ip samples.