GSM4818217: Sample 1B; Homo sapiens; RNA-Seq
Total RNA was extracted with RNeasy mini kit (Qiagen) and quantified by using Nanodrop 8000. cDNA libraries were prepared following Illumina RNA-Seq preparation protocol. The cDNA fragments were amplified by polymerase chain reaction (PCR) and sequenced at both ends using an Illumina Genome Analyzer IIx. Rna-seq and ChIP-seq data was analyzed using MAP-RSEQ (http://bioinformaticstools.mayo.edu/), respectively, high-throughput packages designed by the Mayo Clinic Bioinformatics Program for these purposes.