GSM5548411: ΔadcR strain_rep2; Streptococcus suis; RNA-Seq
RNA was extracted using an Eastep Super Total RNA Isolation Kit (Promega, Shanghai, China), according to the manufacturer's instructions. RNA integrity and concentration were analyzed by gel electrophoresis and spectrophotometric analysis using a Nanodrop 200, respectively. The ribosomal RNA was removed using a rRNA Removal Kit. Then, the mRNA was fragmented into short fragments (200-300 bp) and used as template for cDNA synthesis. The cDNA was added with sequencing adapters and enriched by PCR amplification.