GSM4726288: G2_80kV; Homo sapiens; RNA-Seq
After irradiation and incubation at 37°C for 6h, 2.5ml of blood were transferred to PAXgene™ Blood RNA tubes. Apart from irradiation matching non-irradiated sham controls samples were handled the very same way. RNA Seq library preparation was performed with NebNext Ultra II Directional Kit. Library fragment length was determined with Agilent Bioanalyzer 2100 (Agilent, Santa Clara, CA, USA) and concentration was measured with a Qubit 3.0 Fluorometer (Thermo Scientific, Waltham, MA, USA). Libraries were sequenced with 1 x 75 bp single end reads following the protocols for the NextSeq500 sequencing platform (NextSeq 500 High Output Kit 75bp; NextSeq System Guide; NextSeq Denature and Dilute Libraries Guide; Illumina, San Diego, CA, USA, Document # 15048776 v02, Document # 15046563 v02).