GSM4877999: MES-GFP-BLA-1; Trypanosoma brucei; RNA-Seq
Total RNA was extracted using a Qiagen RNeasy Mini kit with genomic DNA depleted using Turbo DNase (Ambion) according to manufacturer's instructions Each RNA sample will be quantified using Qubit and run on a Tapestation to assess sample integrity. mRNA will be isolated from total RNA using oligo dT beads. After fragmentation the sample will be converted to double stranded DNA and ligated to Illumina adapters. The second strand cDNA is then degraded by the UNG enzyme providing directional information to the library. Indexing of each sample will be performed by PCR.0. Carried out at Imperial College London BRC genomics facility.