GSM4725946: dm-ter-screen-repool-gRNA-m1-1: Replicate embryonic lethal CRISPR-KO screen teratoma 1 10X replicate 1 gRNA reads; Homo sapiens; RNA-Seq
Teratomas were excised and dissociated using the GentleMACS tuomor dissociation protocol, resuspended in PBS-BSA and loaded onto the 10X Genomics Chromium system as per manufacturer's instructions Single cell libraries were constructed with the Single Cell 3' Reagent Kit from 10X genomics