GSM6321951: DSS anti-PD1 ECP - replicate 2; Mus musculus; RNA-Seq
Colitis tissue was digested with the Lamina Propria Dissociation Kit (Miltenyi, 130-097-410) according to manufaturer's instructions. Dead cells were removed with the Dead Cell Removal Kit (Miltenyi, 130-090-101) and CD45 positive cells were isolated using CD45 MicroBeads (Miltenyi, 130-052-301) according to manufacturer's instructions. 10,000 cells were processed into library. 10,000 cells were processed for scRNA Seq using the Chromium Next GEM Single Cell 3' Reagent Kit v3.1 (Dual Index) (10X genomics) Whole mRNA 3' Dual Index