GSM5513416: M14 KO  input; Mus musculus; RIP-Seq
Colonic epithelial cells were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. SMARTer Stranded RNA-seq Kit v2- Pico Input  Mammalian (Cat#634411) was used with 5 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols