GSM5393367: 13393_CS23_P5_ATAC-seq; Homo sapiens; ATAC-seq
50,000 cells were harvested from organoids and lysed in lysis buffer. The lysate was treated in 50 μL reactions with Nextera TDE1 transposase (Illumina, 15027865) for 30 min at 37°C. the transposed DNA was purified using Qiagen MinElute PCR Purification Kit The purified DNA was amplified and indexed using Nextra DNA CD Indexes (Illumina, 20018707)