GSM5089655: WT-A5-NEG-2 - BulkRNA; Mus musculus; RNA-Seq
A ring with width 0.25 mm of skin adjacent to the wound edge of control and Rorgt deficient animals (2 or 3 mice pooled per group per sequencing run) was excised and digested with Librase TL(Roche) . Wound edge epithelium was FACS purified from live Epcam+ cells based on integrin a6 and integrin a5 expression, while excluding CD45+ immune cells, CD31+ endothelial cells and CD117+ melanocytes. Total RNA was extracted from FACS purified integrin a6+ integrin a5+ (migrating tongue) and integrin a6+ integrin a5- (wound edge) samples using RNeasy Plus Micro Kit (Life Technologies) Libraries were generated with SMART-Seq® v4 Ultra™ Low Input RNA Kit for Sequencing and SMARTer® ThruPLEX® DNA-Seq Kit (Takara Bio) flollowing te manufacturer's protocol.