GSM5605688: bone marrow eosinophils_WT_F4; Mus musculus; RNA-Seq
RNA was extracted with TRI Reagent and the RiboPure RNA Purification Kit (ThermoFisher Scientific #AM1924) immediately after sorting. RNA quality and quantity was determined using the Agilent 2100 Bioanalyzer with the Agilent RNA 6000 Pico Kit. Only samples with RIN ≥7 were used for RNA sequencing. RNA samples were converted into cDNA using the NuGEN Ovation RNA-Seq System v2. Illumina-compatible libraries were produced using the NuGEN Ovation Ultralow Library System V2.  Sequencing was performed on the Illumina HiSeq 4000 with single ended reads of at least 75 bp.