GSM5513942: 20 week +dox experimental 2 female [TC44982_CTTGTA]; Mus musculus; RNA-Seq
Tissue was isolated and immediately flash frozen. Total RNA was isolated from individual skeletal muscles using TRIzol reagent (Invitrogen) with a Bullet Blender (Next Advance). RNeasy fibrous tissue mini-kit (Qiagen) was used for RNA extraction of skeletal muscle tissues used in RNA-seq experiments according to the manufacturer's protocol. RNA was resuspended in RNase-free water (Ambion) and treated with DNaseI (Ambion). The Genomic and RNA Profiling Core (GARP) at Baylor College of Medicine (BCM) performed HiSeq library preparation and Illumina sequencing of 100-bp paired-end reads on a HiSeq 2500 sequencer. RNA was poly-A selected, barcoded and run in eight lanes.