GSM5089967: Eosinophils_isotype_rep1; Mus musculus; RNA-Seq
RNA was isolated by phenol/chloroform extraction using a commercial RNA Isolation kit (SIGMA-ALDRICH 83913) according to manufacturer's instructions except that volumes were downscaled to 1/6 of recommended volume, samples were sheared through 27G cannulas during initial lysis and precipitation was performed at -80°C instead of -20°C. Then RNA was dissolved in DEPC H2O. RNA was sent to Novogene Europe (Cambridge, UK) for sequencing as a service. There, library preparation was performed with NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to mark sequences of individual samples.