GSM5582132: Nmus_smooth_3; Neisseria musculi; RNA-Seq
Each pellet was suspended inTris EDTA buffer pH 8.0 containing of lysozyme, incubated for 5 min at room temperature with vortexing every 1 min. RNA extraction was done using RNeasy Mini kit (Qiagen) and contaminating DNA was removed by DNase digestion using the Turbo DNA-free kit (Invitrogen). Libraries were constructed using Illumina Truseq Stranded mRNA Library Prep kit.