GSM5226601: LacZ_scATAC; Mus musculus; ATAC-seq
For direct cardiac reprogramming, pMXs retrovirus encoding Mef2c, Gata4, Tbx5, and LacZ were packaged by transfecting platE cells with Trans-IT as the manufacturer suggested. Viruses were collected from one 10-cm dish were resuspended in 100μl iCM medium(400ml DMEM, 100ml M199, and 50ml FBS). All virus transductions were performed in the iCM medium containing 4 μg/ml of polybrene. For single-cell ATAC-seq, cardiac fibroblasts were transduced with M, G, and T viruses for reprogramming sample or equal amounts of LacZ for mock sample. Illumina libraries were prepared using Chromium Single Cell ATAC v1.0. single-cell ATAC-seq