GSM5087965: Mpbes1_rep3; Marchantia polymorpha; RNA-Seq
RNA extraction from thalli (ca. 100mg including apical notches) was done using the Rneasy plant mini kit (Qiagen 74904). To remove contaminating DNA, the TURBO DNAfreeTM Kit. (Ambion AM1907) was used according to the manufacturer's recommendations.  Quality of RNA was checked with Bioanalyzer. All samples had a RNA Integrity Number (RIN) > 7. TruSeq Stranded mRNA Sample Prep kit