GSM5001897: Jurkat_RNA_PBIT_24h; Homo sapiens; RNA-Seq
The extraction of total RNA was performed using the RNeasy Plus Mini kit (Qiagen) according to the protocol recommended by the supplier. Total RNA was quantified using a Nanodrop 1000 spectrophotometer (Thermo Scientific) and stored at −80 °C until needed. Extracted RNA was used for the RNA-seq library preparation, using the TruSeq RNA Library Prep Kit v2 (Illumina). Libraries were paired-end sequenced on the Illumina NextSeq 500 sequencer. Extracted RNA was used for the RNA-seq library preparation, using the TruSeq RNA Library Prep Kit v2 (Illumina). RNA-Seq libraries were paired-end sequenced (50+30 bp) on an Illumina NextSeq 500 sequencer.