GSM5291770: LRB444 irld-39(duk1); irld-52(duk17) rep2; Caenorhabditis elegans; RNA-Seq
RNA was isolated using TRIzol following the manufacturer's protocol with notable exceptions. Only 100 uL of Trizol was used, and 5 ug linear polyacrylamide (Sigma Aldrich) was included as a neutral carrier for RNA precipitation. RNA was eluted in nuclease-free water and incubated at 55°C for approximately four minutes to resuspend the pellet. NEBNext Ultra II RNA Library Prep kit was used to perform polyA selection on total RNA and prepare libraries for sequencing. ThermoFisher ERCC Spike-In mix was added based on total RNA. The libraries were enriched with PCR