GSM5856535: Resident, MID49, 5O; Mus musculus; OTHER
RNA was isolated using RNeasy Micro Plus Kit  (Qiagen) according to manufacturer's instructions Library preparation was performed using the template switch technique and UMI-adaptor incorporation during the TCR-specific cDNA synthesis (Mint-2 cDNA synthesis kit, Evrogen).  This was followed by two nested PCRs and a third amplification for the Illumina adaptor incorporation. DNA was purified using MinElute PCR Purification Kit (Qiagen) RNA-Seq (RACE)