GSM4878384: Female brain; Aedes aegypti; RNA-Seq
Brain was dissected from 30 males and females, resuspended in Schneider's Drosophila Medium on ice, and subjected to single nucleus isolation using the Pure Prep Nuclei Isolation kit (Sigma, St. Louis, MO, USA). Complete brain tissue was resuspended in Schneider's Drosophila Medium on ice. Chromium Next GEM Single Cell 3ʹ GEM, Library & Gel Bead Kit v3.1 protocol