GSM5514569: blood sample from donor 1132 2 weeks post vaccination [VIC008846]; Homo sapiens; RNA-Seq
Total RNA was extracted from whole blood collected in PAXgene Blood RNA tubes (cat# 762165) using Qiagen PAXgene Blood RNA kits (cat# 762164) and subjected to the ThermoFisher GlobinClear kit (cat# AM1980). cDNA was generated from 10 ng of RNA using the Takara Bio SMART-Seq v4 UltraLow Input RNA Prep kit (cat# 634891) per manufacturer's suggestions, with control RNA spiked-in (Life Technologies ERCC Mix 1 cat# 4456740). Sequencing libraries were generated using the Illumina Nextera XT DNA Sample Prep kit (cat# FC-131-1096) according to manufacturer's instructions and sequenced on Illumina NovaSeq 6000 with S2 Reagent Kit, 300 cycles (cat# 20012860)