GSM5579258: Oocytes XX rep3; Mus musculus; RNA-Seq
Total 30 oocytes (10 oocytes of 50-55 µm and 20 oocytes of 55-60 µm diameter) per sample were pooled in 10 µl lysis buffer (12 µM polyT primer, 0.4% Triton-X 100, 10 mM each dNTP, RNase inhibitor and ERCC) in biological triplicate for each genotype. Lysis was carried out in a thermocycler at 72C for 3 min, 4C for 10 min, and 25C for 1min, and the lysed samples were stored at -80C. RNA libraries were prepared using an in-house Smartseq3 protocol, with modified oligo-dTVN: 5'-AGATGTGTATAAGAGACAGN12ACT30VN-3' and Template Switching Oligo (TSO): 5'-AGATGTGTATAAGAGACAGN12ACGCrGrGrG-3'