GSM6321792: Differentiated root, 45 days after fertilization [SAMPLE6_DT]; Ceratopteris richardii; RNA-Seq
Plant organs were grounded in the presence of liquid nitrogen to a fine powder. Samples were transferred to PureLink® Plant RNA Reagent and mixed by inversion until no visible clumps of tissue were observed. We followed the recommended method from the manufacturer, except for the overnight incubation of the aqueous phase with isopropyl alcohol at 4 ºC. RNA was isolated from plant samples with the to PureLink® Plant RNA Reagent. Samples were resuspended in 50 uL of DEPC-treated water. We sent at least 3 ug of RNA per sample to the Laboratorio de Servicios Genómicos (LABSERGEN) from the Advanced Genomics Unit (LANGEBIO) for library preparation and sequencing. RNA libraries for RNA-seq were prepared using TruSeq RNA Library Prep Kit v2 following manufacturer's protocols.