GSM6323655: Meningeal Endothelial cells, WT, Batch1; Mus musculus; RNA-Seq
The spinal cord and surrounding meninges were dissected, pooled according to genotype, and dissociated with Papain Dissociation System (Worthington Biochemical). GFP+ motor neurons and Cherry+ endothelial cells were isolated on a BD Influx Cell Sorter and collected in RLT lysis buffer (Qiagen) containing β-mercaptoethanol. RNA was extracted with RNeasy Mini Kit and on-column DNase digestion (Qiagen). Stranded mRNA libraries prepared with Illumina TruSeq RNA Library Preparation Kit (v2)