Illumina HiSeq 2500 paired end sequencing; Bulk RNA-seq of fibroblasts and mesothelial cells from healthy mouse tissues
Tissues were digested and CD45-CD31-Epcam-PDPN+PDGFRA+ (fibroblasts) or CD45-CD31-Epcam-PDPN+PDGFRA- mesothelial cells were sorted directly into TRIZOL. RNA from FACS sorted cells was sourced from the following mouse tissues: Liver, Lung, Lymph Node, Spleen, Bone, Pancreas, Omentum, Mesentery, Epididymal, Inguinal Adipose, Brown Adipose Tissue. RNA was isolated as described (Painter et al., 2011, J. Imm.) or at Expression Analysis, Inc. Paired-end RNA-seq libraries were constructed using the SMARTer Low Input RNA Kit (Takara.