GSM6326642: Rep3, BF, RNAseq, set27, SKO; Trypanosoma brucei brucei; RNA-Seq
RNA was extracted from 1-4 x 10^7 cells using Trizol (Thermo Fisher Scientific) according to the manufacturer's protocol. The RNA was treated with DNase and then purified using Phenol/Chloroform/Isoamyl alcohol pH 4. Libraries were constructed using NEBNEXT Ultra II Directional RNA Library Prep kit (NEB) with a Poly-A mRNA magnetic isolation module (NEB).