GSM5457927: Tumor Sample 6; Homo sapiens; MeDIP-Seq
Ovarian tissue samples were extracted, washed in PBS, and homogenized in SET buffer. Further homozenate was treated with protenase K and 10% SDS followed by overnight incubation at 37 ºC. Next, phase sepration and precipitation in 3M sodium acetate and chilled absolute ethanol. further precipitate was subjected to centrifugation, supernatant was discarded and pellet was retained and air dried overnight and resuspended in 150 µl of TE Buffer and stored in 4 ºC till further use.   Total 1µg of genomic DNA was used to prepare MeDIP seq library using  Illumina TruSeq Nano DNA Library Prep Kit and MagMeDIP Kit (Diagenode, USA; Cat. No. C02010020). DNA libraries were prepared for sequencing following standard Illumina protocol