GSM5578479: ATAC_H1_4C_rep2; Homo sapiens; ATAC-seq
The suspension of H1 cell nuclei was then centrifuged for 5 min at 500g at 4 °C, followed by the addition of 40 μl Tn5 transposase reaction mix (20 μl 2x TD buffer, 0.4 μl 1% Digitonin, 0.4 μl 10% Tween 20, 4 μl 3×PBS, 13.2 μl nuclease-free H2O and 2 μl Transposome). The transposition reaction was incubated at 37°C for 30 min.Equimolar Adapter1 and Adapter 2 were added after transposition. PCR was then performed to amplify the library. After the PCR reaction, libraries were purified with the AMPure beads and library quality was assessed with Qubit. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumina) according to the manufacturer's instructions.After cluster generation, the library preparations were sequenced on an Illumina Hiseq platform and 150 bp paired-end reads were generated (Novogene, Beijing, China).