GSM5457106: S1785_00035_S1_R; Homo sapiens; RNA-Seq
Core biopsies were taken under image guidance from breast tumours before, during and after chemotherapy treatment. Pathology review was conducted to identify tumour areas within each core, which were subsequently macrodissected under RNAse-free conditions. Following RNA extraction, total RNA was amplified using the NuGEN WT-Ovation FFPE RNA amplification System V2. Libraries were prepared for sequencing using the Illumina Tru-Seq RNA Exome library preparation kit and standard Illumina protocols