GSM5267073: WT4; Mus musculus; RNA-Seq
Th9 cells were generated in vitro from naïve CD4+ T cells that were isolated from the murine spleen using the naive CD4+ T cells isolation kit from Miltenyi Biotec. Cells from 6 samples in total (3*WT, 3*Cblb-/-) were polarized into Th9 cells (3ng/ml hTGFβ, 20ng/ml IL-4 and 20µg/ml anti-IFNγ) for 48h and purified using CD4 (L3T4) MicroBeads from Miltenyi Biotec. Library preparation was performed using the 10x Genomics Single Cell 3' reagent Kits v3 according to manufacturer's specifications. single-cell RNA-seq