Illumina HiSeq 1500 sequencing; Gain-of-function CRISPR screens identify tumor driver genes conferring melanoma cell plasticity and therapy-resistance
Genomic DNAs from cell pellets (>36.106 501Mel cells) and tumors (>400mg). Genomic DNAs from cell pellets and tumors were extracted using the Zymo Research Quick-gDNA MidiPrep according to the manufacturerâ€™s protocol. PCR have been performed using the NEBNextHigh Fidelity 2X Master Mix. 32 cycles/single-step reaction according to Joung, Nature Protocols  2017. Purify pooled PCR products using Zymo spin V with reservoir PCR products have been separated on agarose Gel (2%) to eliminate nucleotides and non-desired amplicons. PCR products have been Gel extracted using Zymoclean Gel DNA Recovery Kit - Uncapped columns