GSM5184198: CAG-Dmrt1 1 DPTx rep2; Mus musculus; RNA-Seq
Cells or tissues were harvested into 1 ml Trizol reagent and stored at -80o C.  Total RNA was prepared according to Trizol reagent manufacturer's protocols and further cleaned up using RNAeasy kit protocols and reagents (Qiagen).  RNA was quantified via Qubit RNA assay, and 500 ng total RNA per sample were used in stranded mRNA-seq library preparation (KAPA Biosystems, KK8481) for Illumina sequencing.