Illumina HiSeq 2500 sequencing; RNA-seq to investigate gene expression after HCV eradication with IFN therapy in HIV/HCV-coinfected patients
Peripheral venous blood samples were collected in EDTA tubes. Total RNA was extracted from Peripheral Blood Mononuclear Cells with the RNeasy minikit (Qiagen™), according to the manufacturer's instructions.  Libraries were synthesize with the Illumina's TruSeq Stranded mRNA Sample Prep Kit v2 using 500 nanogram of total RNA following manufacturer's protocol.