GSM5598289: input; Drosophila melanogaster; ChIP-Seq
Samples were cross-linked in 1.8% formaldehyde and then were sonicated to produce DNA fragments with an average size of ~500 bp. Sonicated lysates were added to Dynabead protein G coupled with α-Yorkie antibodies and rotated overnight at 4 °C. The chromatin samples were reverse cross-linked at 65 °C for 16 hours. Immunoprecipitated DNA was prepared for next generation sequencing using the Epicenter Nextera DNA Sample Preparation Kit.