GSM4876963: NCI-H2291_replicate03; Homo sapiens; ssRNA-seq
Whole-cell RNA was isolated using RNeasy Mini columns (Qiagen). Total RNA (5 μg) from each sample were depleted of rRNA using the Ribo-Zero Gold rRNA Removal Kit for human, mouse and rat RNA (Illumina). 20 ng of RNA input was used to generate the RNA-seq libraries using the Agilent Sure Select Strand Specific RNA Library Prep for Illumina Multiplex Sequencing Version C.0 (Illumina). The single-stranded RNA library was sequenced on a HiSeq 2000 V4 (Illumina) with 150 bp paired-end reads.