GSM5460420: shQSER1-2_P53; Homo sapiens; ChIP-Seq
ChIP was performed according to the previously published protocol (Guo et al.,2020). Briefly, HCT-116 cells were crosslinked with 1% formaldehyde (sigma) for 10 min and quenched with glycine (sangon biotech) for 5 min at the room temperature. Fixed cells were sonicated and immunoprecipitated with the mixture of  ProteinA Agarose (ROCHE) and antibody overnight at 4 ℃. ChIP-DNA was purified using PCR purification kit (Qiagen) and performed for qPCR using iTaq™ Universal SYBR® Green Supermix (Bio-Rad) on CFX96 (Bio-Rad). The ChIP-seq sample preparation kit was used to prepare generate ChIP-seq library. NEBNext® DNA Library Prep Master Mix Set for Illumina