GSM4878254: WTcre.2; Mus musculus; RNA-Seq
IgA plasma cells were FACS-purified from 2 pooled mLN per sample (CD138+ TACI+ IgA+ CD19+ B220+) and sorted directly into Trizol (Qiagen) Lysis reagent. Total RNA was isolated with the RNeasy Micro Kit (Qiagen). Sequencing libraries were constructed with the Takara Clontech Smart-Seq v4 Low input kit according to manufacturers instructions and sequenced on Illumina Hiseq X instruments.