GSM5950074: F1G.3; Mus musculus; RNA-Seq
For bulk tissue RNAseq, RNA extraction was performed using the RNeasy Micro Kit (Qiagen) following manufacturer instructions. For cell-type-specific RNAseq, RNA was extracted from frozen TRIzol LS (Ambion) homogenates using the Direct-zol RNA Microprep Kit (Zymo Research) following manufacturer instructions. For bulk tissue RNAseq, Sequencing libraries were prepared by Genewiz/Azenta with polyA selection and sequenced with Genewiz/Azenta on an Illumina HiSeq 4000 machine using a 2 × 150 bp pair-end read configuration to a minimum depth of 20 million reads per sample. For cell-type-specific RNAseq, ribo-depleted sequencing libraries were prepared with the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (TaKaRa Biotech) following manufacturer instructions and sequenced with Genewiz/Azenta on an Illumina NovaSeq S4 machine using a 2 × 150 bp pair-end read configuration to a minimum depth of 40 million reads per sample.