GSM5733762: P003_pre_TCR [Batch5]; Homo sapiens; OTHER
Chromium Single cell 5' Library and Gel Bead Kit V3 (10X Genomics) was used for scRNA-seq and TCR sequences libraries construction. Cell-hashing antibody (HTO)-labeled cells from 2 samples were pooled together and washed with RPMI-1640 immediately before loading on the 10X controller.Then library was constructed following the manufacturer's protocol, with some additional steps for the amplification of HTO barcodes.