GSM5267597: sop1-5 R2 Exp3; Arabidopsis thaliana; ncRNA-Seq
inflorescences were collected and snap-frozen in liquid nitrogen. Total RNA was extracted with Trizol reagent according to the instructions from the manufacturer. After the RNA extraction, the RNA was treated for polysaccharides with an additional mild EtOH precipitation. 1 µg of total RNA was used as input per sample. The libraries were constructed with the NEBNext small RNA libraries preperation kit #E7300 following the protocal of the kit. miRNA-Seq (or rather small RNA seq as you do not only sequence miRNAs)