GSM6321826: BMDM of WT, LPS/MSU, 2; Mus musculus; RNA-Seq
Total RNA was extracted with TRIzol reagent according to the manufacturer's instructions (Invitrogen). The RNA concentration was calculated by Quant-IT RiboGreen (Invitrogen, R11490). To assess the integrity of the total RNA, samples were run on the TapeStation RNA system (Agilent Technologies, Santa Clara, CA, USA, 5067-5576). Only high-quality RNA preparations with RIN greater than 7.0 were used for RNA library construction. RNA-seq libraries were prepared according to the TruSeq Stranded mRNA Sample Preparation Guide, Part #15031047 Rev. E (Illumina) and were then submitted for paired-end (2×100 bp) sequencing on an Illumina NovaSeq system (Illumina, Inc., San Diego, CA, USA) by Macrogen Incorporated (https://www.macrogen.com/).