GSM5393490: 20-Neck; Homo sapiens; RNA-Seq
The samples were frozen in the RNALater buffer and stored at -80oC. RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit was used with 1 ug of total RNA for the construction Sample Prep. Kit : QIAseq FastSelect RNA Removal Kit_Human Cyto & Mito rRNA + Illumina TruSeq Stranded mRNA Sample Prep Kit + limited cycles of PCR by KAPA HiFi Polymerase