GSM5457053: Microglia_Plcg2_WT_Rep1; Mus musculus; RNA-Seq
Total RNA was extracted from cell pellets using RNeasy Mini Kit (QIAGEN, #71104) with RNase-free DNase set (QIAGEN, #79254) for on column DNA digestion according to manufacturer's instruction. RNA samples were sent to Genewiz (https://www.genewiz.com/) for library preparation via polyA selection. Genewiz (https://www.genewiz.com/) performed 150-bp paired-end sequencing on Illumina HiSeq to obtain ~20 million reads per sample.