GSM5267696: Input Smad4 replicate2; Mus musculus; ChIP-Seq
For Gli3 ChIP, briefly limb buds at E10.5 and E11.5 were collected and pooled into ice-cold PBS prior to crosslinking them in 1% formaldehyde/PBS for 20min at room temperature and processed for ChIP as previously described in Peterson et al. 2012. For Smad4, embryos were cross-linked with 1%formaldehyde and chromatin immunoprecipitation was performed according to Sheth et al 2016. For Gli3 ChIP, sequencing libraries were prepared according to manufacturer instructions (Takara Bio, Japan) and sequenced using a NextSeq instrument (Illumina) to generate single end reads. While for Smad4 ChIP, libraries were prepared with MicroPlex Library preparation kit V2 (Diagenode) according to the manufacture's instructions/protocol, and  sequenced using a NextSeq instrument (Illumina) to generate paired end reads.