GSM5605594: H3K4me1_T08_rep1; Homo sapiens; ChIP-Seq
For ChIP, cells were sonicated to produce chromatin fragments of ~200 base-pairs, lysates were clarified by sedimentation and histone-DNA or Lamin-DNA complexes were isolated from supernatants by ChIP using specific antibodies. RNA was purified from cell lysates using the RNEasy mini kit (Qiagen). ChIP-seq and RNA-seq sequencing libraries were prepared according to  Illumina protocols for sequencing on a Nextseq500 or Novaseq (ChIP-seq) or on a Nextseq500 (RNA-seq) at the Norwegian Sequencing Center.