GSM5267741: RNAseq of CD34_EV_3; Homo sapiens; RNA-Seq
RNA was harvested using the Qiagen AllPrep Kit and RNA was quantified using the Quant-iT RiboGreen RNA assay (ThermoFisher) and quality checked by the 2100 Bioanalyzer RNA 6000 Nano assay (Agilent) or 4200 TapeStation High Sensitivity RNA ScreenTape assay (Agilent) prior to library generation. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit according to the manufacturer's instructions (Illumina, PN 20020599). RNAseq: paired end 100 cycle sequencing was performed on a NovaSeq 6000 (Illumina).