NextSeq 500 paired end sequencing; Comparative transcriptome analysis of the regenerating zebrafish telencephalon unravels a key regulatory role for Wnt/β-catenin signaling at the early wound healing stage
Tissues were dissected under microscopy. Adult zebrafish were fed under normal conditions 4-(1,3,3a,4,7,7a-Hexahydro-1,3-dioxo-4,7-methano-2H-isoindol-2-yl)-N-8-quinolinyl-Benzamide (IWR-1) was added to fish water at 20 micromolar. Dissected tissues were immersed in RNA later, and Total RNA isolation was performed. RNA-seq libraries were built using the TruSeq RNA Library Preparation v2 Kit (Illumina)