GSM5856579: CFS414 cell line, passage 24, 10x seq; Taeniopygia guttata; RNA-Seq
Confluent CFS414 cells were dissociated from the surface of the dish using 0.25% Trypsin-EDTA, washed with PBS three times and counted on a Countess II FL (Life Technologies). A single cell suspension was diluted to around 1,000 cells/µL in 1xPBS. Approximately 5,000 cells were run on a Chromium Chip (10x Genomics). cDNA synthesis and library preparation was performed using the Chromium Single Cell 3' Reagent Kits (version 3, 10x Genomics) according to manufacturer's instructions. Sequencing was performed by Novogene Co., Ltd. on an Illumina HiSeq 4000.