GSM4725778: FLT3LcDC2_Alum_G04; Mus musculus; RNA-Seq
RNA was extracted using RNeasy Plus Micro kit cDNA was produced from isolated RNA with the Smart-Seq2 reverse-transcription protocol as described by Picelli et al.3 with the following modifications: 1) Concentrations of input RNA was normalized to ~2,000 cells-worth of input RNA per reaction. 2) The Superscript II reverse-transcription enzyme was replaced with Superscript III (ThermoFisher, #18080-085) which was applied according to manufacturer's instructions. Paired-end sequencing libraries were generated from cDNA with the Nextera XT DNA sample Prep Kit (Illumina, #FC-131) according to the manufacturer's instructions. Libraries were pooled at an equimolar ratio and sequenced on a NextSeq500/550 sequencer (Illumina) using a 75 cycle v2.5 sequencing kit with a paired end read structure.