GSM6322576: TGFB1-10ng/ml treated day 25 OS-HLOs, replicate 2, scRNAseq; Homo sapiens; RNA-Seq
Freshly collected human liver organoids were washed 1X with warm DPBS(-/-) and briefly dissociated by incubation with 0.05-0.25% Trypsin-EDTA for 10 minutes at 37°C.   Day 34 ULA- and day 25 OS-HLOs underwent an additional DBPS (-/-) wash and 10 minutes of trypsinization to allow complete digestion. For each replicate, full single cell dissociation was confirmed manually by light microscopy. After a final DPBS(-/-) wash, organoids were resuspended in DPBS(-/-), counted after with the TC20™ Automated Cell Counter (Trypan Blue) and transferred on ice for library preparation. Library was performed according to the manufacter's instructions (single cell 3' v3 protocol, 10x Genomics).