GSM5579248: MO204 rep3; Bombyx mori; RNA-Seq
Silk glands were dissected, flash frozen on dry ice, total RNA extracted from 100 µg tissue with TRIzol reagent. RNA purified with Turbo DNAse (Ambion) and RNA Clean and Concentrator (Zymo Research). RNA libraries were constructed using the TruSeq Stranded RNA Library Prep Kit (Illumina) following the manufacturer protocols.