GSM5088599: TNF-1; Homo sapiens; RNA-Seq
cultured cells were directly lysed in RNA lysis buffer, followed by RNA isolation using the Qiagen Mini kit with a DNA digestion step (Qiagen) Sequencing libraries were prepared using the Smart-seq2 protocol (Picelli et al., 2013) (with in-house indexing primers) RNA-sequencing, Libraries were sequenced using an Illumina HiSeq4000 with 75bp paired-end sequencing (Lamble et al., 2013).