GSM5605694: KD2; Homo sapiens; RNA-Seq
Treated and untreated JEG-3 cells were harvested in 350µL of buffer RLT plus. Successive RNA extraction was performed using the AllPrep DNA/RNA/miRNA Universal Kit according to the manufacturer's protocol. RNA was quantified using a NanoDrop 1000 spectrophotometer (Thermo Scientific, Waltham, MA). RNA was then converted to cDNA, the next step toward analyzing gene expression. Treated and untreated samples of JEG-3 RNA previously extracted using the AllPrep DNA/RNA/miRNA Universal Kit were submitted to the High Throughput Sequencing Facility at UNC Chapel Hill for RNA sequencing. Total RNA samples were submitted for sequencing using the HS4000 HO platform. Samples were sequenced in duplicate, and libraries were prepped with the Kapa Stranded mRNA-Seq kit from Illumina Platforms. Sequencing was performed after all samples passed QAQC, with a paired-end read type, with a read length of 2x75.