GSM6322871: e2.TCM.TMAO.1; Mus musculus; RNA-Seq
Total RNA was extracted from BMDM or sorted F4/80+CD11b+ TAMs using RNeasy Plus Micro Kit (Qiagen, 74034). RNA quality was validated using the TapeStation High Sensitivity RNA ScreenTape (Agilent) Libraries were prepared using Illumina Stranded mRNA Prep kit (500 ng DNase1 treated total RNA was used)