GSM5578496: 4BII; Solanum tuberosum; RNA-Seq
Total RNA was isolated using TRIZol method as described Chomczyński and Sacchi (1987). The mRNA was isolated using Dynabeads®mRNA Purification Kit for mRNA enrichment (Ambion, 61006), and cDNA libraries were prepared using using MGIEasy RNA Directional Library Prep Set (MGI, 1000006386). The established cDNA libraries were sequenced on the BGISEQ-500 sequencing platform (BGI Genomics, China) to generate 100-bp paired-end reads (PE100).