GSM5088222: 18_N; Homo sapiens; RNA-Seq
Cell populations from tissue or blood were FACS-sorted directly into 300µl of RLT lysis buffer (Qiagen) and 20µM DTT (Sigma). RNA was isolated using the Qiagen Mini kit with a DNA digestion step (Qiagen). Sequencing libraries were prepared using the the Smart-seq2 protocol (Picelli et al., 2013)  (using in-house indexing primers) RNA-sequencing, Libraries were sequenced using an Illumina HiSeq4000 with 75bp paired-end sequencing (Lamble et al., 2013).