GSM4818242: Sample_03_blood; Homo sapiens; RNA-Seq
Lymphoid cells sorted from blood of 3 CLM patients were subjected to scRNA-seq analysis. CD3+ FACS-sorted cells were counted with an automatic cell-counter (Countess II, Thermo Fisher) and loaded into one channel of the Single Cell Chip A using the Single Cell reagent kit v3 (10X Genomic) from gel bead emulsion generation. Following capture and lysis, cDNA was synthesized and amplified for 14 cycles following the manufacturer's protocol. 50 ng of the amplified cDNA were then used for each sample to construct Illumina sequencing libraries. Sequencing was performed on the NextSeq550 Illumina sequencing platform following 10xGenomics instruction for read generation, reaching at least 35000 reads as mean reads per cell.