GSM5291259: Day_12_FSHD2_2_R1; Homo sapiens; RNA-Seq
Cells were lifted using trypsin. Total RNA was extracted by using the RNeasy kit (QIAGEN). RNA was converted to cDNA using the Smart-Seq 2 protocol (Picelli, et al., Nat. Protoc., 2014). Libraries were constructed with the Nextera DNA Library Prep Kit (Illumina) for days 0 to 5 for FSHD2-3. The Nextera DNA Flex Library Prep Kit (Illumina) was used for all other RNA-seq samples. Libraries were sequenced on the Illumina NextSeq500 with paired-end 43 bp reads to a depth of 5 to 40 million reads.