GSM5226128: TSC2+/- kidney organoids-rep 1; Homo sapiens; RNA-Seq
For each sample, total RNA was extracted from 5 organoids using the RNeasy Plus MiniKit (Qiagen). Purity was determined byA260–A280. RNA Integrity Number or RNA Quality Number were determined using a Agilent Bioanalyzer and a Fragment Analyzer respectively. Copy DNA (cDNA) library preparation and sequencing was performed at the Molecular Biology Core Facilities at Dan- Farber Cancer Institute RNA libraries were prepared for sequencing using the Roche Kapa mRNA Hyper Prep