GSM5225966: LT_DT_scRNA; Mus musculus; RNA-Seq
Tissues were harvested in PBS on ice and single cells were prepared by using the mouse GentleMacs kit. Single cells were frozen in 10%DMSO+90% FBS. When cells were thawed, 99% live cells were sorted by BD sorter using DAPI staining. 10,000 cell were targeted for 10X Genomics sequencing. Libraries were prepared using Chromium single cell 3' reagent kit version 2 for Dormant, Primary, Recurrent and LT-DT samples, and version 3 for IgG and Jag1 samples.