GSM6208707: GFP-negative cells, IMR90-4 line; Homo sapiens; RNA-Seq
RNA extraction was performed using the RNeasy Plus Micro Kit (Qiagen). Lysates were thawed on ice, processed through gDNA Eliminator spin columns, processed through RNeasy MinElute spin columns per manufacturer instructions, and eluted into RNase-free water. RNA quality control, library preparation, and sequencing were performed by Novogene Corporation (Sacramento, CA). RNA quantity was assessed using a NanoDrop spectrophotometer; RNA quality was assessed using an Agilent 2100 Bioanalyzer. Poly(A) mRNA enrichment was performed using poly(T) oligo-conjugated magnetic beads, first-strand cDNA synthesis performed using random hexamer primers, second-strand cDNA synthesis performed, and libraries prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs).