GSM5543485: KO Naïve #1 (18010X2); Mus musculus; OTHER
DNA was harvested from FACS sorted cells using Dneasy Blood and Tissue Kit (Qiagen). DNA inputs of 200ng were fragmented by sonication (Covaris) Unmethylated cytosines were converted to uracils and sequencing libraries were created using the NEBNext Enzymatic Methyl Seq kit (New England Biolabs) according the manufacturer's instructions. DNA libraries were sequenced using an Illumina NovaSeq 6000 system following the manufacturer's protocols.