GSM5291815: PP_A5-KO_3h_rep2; Homo sapiens; RNA-Seq
Cells were havested and total RNA was isolated using Fast Pure Cell Total RNA Isolation Kit (Vazyme). 1 μg RNA per sample were used as input material for the RNA sample preparations. Each RNA sample was spiked in with an appropriate amount of either Mix1 or Mix2 according to Life Technologies' guidelines. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.