GSM5513960: BS_ddm1ibm1_rep1; Arabidopsis thaliana; Bisulfite-Seq
Genomic DNA was isolated from rosette leaves using the Nucleon Phytopure genomic DNA extraction kit (GE Healthcare). Genomic DNA was fragmented using Covaris S220, and the size between 300-450bp were gel-purified. The libraries were prepared using TruSeq DNA LT Sample Prep Kit (Illumina) and subjected to bisulfite conversion using MethylCode Bisulfite Conversion Kit (Life Technologies). Bisulfite-treated DNA were amplified with KAPA HiFi HotStart Uracil ReadyMix (Kapa Biosystems), and then purified with Agencourt AMPure XP (Beckman Coulter). WGBS: Plants  were sown on MS medium with 1% sucrose and 0.8% agar and then  grown on soil  with 16h light / 8h dark.