GSM5820805: Chronic Therapeutic_TG3; Rattus norvegicus; RNA-Seq
Total RNA was extracted from the trigeminal ganglia                     collected from the hormone treated rats using the Qiagen RN-easy kit protocol.                     RNA was quantified using a Nanodrop™ spectrophotometer (ThermoFisher Scientific,                     Waltham, MA). RNA quality was confirmed by A260/280 ratio and only the samples                     that had a ratio of =2 and a concentration > 20 ng/µL were sent for                     sequencing to Novogene. Novogene confirmed the RNA purity and integrity of the                     samples using a NanoPhotometer™ spectrophotometer and the RNA Nano 6000 Assay                     Kit (Bioanalyzer 2100 system), respectively. Samples with RNA integrity number                     (RIN) > 8 were used for library preparation and sequencing on an Illumina                     platform (HWI-ST1276). A total amount of 1 µg RNA per sample was used for the                     RNA sample preparations. Sequencing libraries were generated using NEBNext®                     UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's                     recommendations and index codes were added to attribute sequences to each                     sample. PCR products were purified (AMPure XP system) and library quality was                     assessed on the Agilent Bioanalyzer 2100 system.