GSM5226377: siccna1_rep1; Homo sapiens; RNA-Seq
RNA extractions were performed from control and CCNA1 knock down FaDu cells in independent triplicates for each condition. RNA was extracted from each sample directly after cell harvesting with TRIZOL, following the manufacturer's protocol (AMBION). RNA pellets were dissolved in RNAse-free water, and then cleaned up on QIAGEN columns using the RNA Clean Up protocol of RNeasy Mini Kit (QIAGEN), which includes an on column-DNase treatment of 15 minutes. RNA was eluted with 40 µl of RNAse-free water and its concentration measured with Nanodrop. RNAseq was subcontracted to BGI Tech Solution (Hong Kong). For each sample, 1 µg RNA was used for libraries preparation with LncRNA library(H/M/R) according to the manufacturer's instructions and sequenced on a DNBSEQ PE100 Eukaryotic Long Non-Coding RNA platform.