GSM4818259: AAV-17; Mus musculus; RNA-Seq
RNA was harvested using Trizol reagent and the KAPA Stranded RNA-Seq Library Prep Kit (Illumina) was used with 1-2 ug of total RNA for the construction of sequencing libraries. Approximately 1-2 µg of total RNA isolated from each independent biological sample was processed with a Ribo-Zero Magnetic Gold kit (Epicentre Biotechnologies) to reduce ribosomal RNA abundance. Sequencing libraries were then constructed using the KAPA Stranded RNA-Seq Library Prep kit (Illumina). The quality of the constructed sequencing libraries was analysed using an Agilent 2100 Bioanalyzer. Quantitative real-time PCR was performed to quantify the sequencing libraries, and the mixed different sample libraries were sequenced using an Illumina HiSeq 4000 platform.