GSM5227104: EvoCrp3_2_chemostat; Escherichia coli str. K-12 substr. MG1655; RNA-Seq
Total RNA extraction was done using the TRIzol-chloroform method. DNase treatment was done to remove any DNA contamination after which enrichment for mRNA was done using the MicrobExpress Kit. Single-end, strand specific libraries for RNA sequencing were prepared using NEBNext Ultra II Directional RNA library kit.The sequencing was carried out on HiSeq 2500 Rapid Run Mode using 1x50 bp single-end reads.