GSM5960339: 35; Mus musculus; miRNA-Seq
Briefly, using TRIzol reagent to extract the total RNA from the hippocampus in accordance with the manufacturer's instructions. The NEB Next Ultra Directional RNA Library Prep Kit for Illumina (NEB, MA, USA) was used to establish an RNA library and assessed the RNA library quality and quantity via Agilent 2100 Bioanalyzer. The RNA library was used for sequence analysis. The sequence analysis was conducted by a NextSeq 500 platform (Illumina, CA, USA). The clean reads were filtered out from raw reads by FastQC and selected for further bioinformatics analysis.