GSM4726729: 48 hours after HCV infection in Huh7.5.1 cells; Homo sapiens; ncRNA-Seq
Total RNA was extracted from cell or animal tissue by Trizol reagent (Invitrogen) separately. The RNA quality was checked by Bioanalyzer 2200 (Aligent) and kept at -80℃.The RNA with RIN >8.0 is right for cDNA library construction. smallRNA libraries were prepared for sequencing using standard Ion  Proton Protocols