GSM5580153: FACS endothelial cells Aged Sham 4; Mus musculus; ATAC-seq
Lungs were perfused, harvested and processed for FACS. Freshly isolated lung endothelial cells were sorted diretly in tubes containing 500 ul of a specific ATAC-seq buffer and transferred for the analysis The size of library DNA was determined from the amplified and purified library by a Fragment Analyzer, and the enrichment of accessible regions was determined by the fold difference between positive and negative genomic loci using real-time PCR.