GSM4876610: PBAT_sperm_hypoxia_3; Danio rerio; Bisulfite-Seq
Total RNA from 6 whole fry (3 control offspring, 3 treatment offspring) was extracted using a Zymo Duet extraction kit (Zymo, NZ). Total nucleic acids for methylation analysis were purified using the Bio-On-Magnetic-Beads (BOMB) approach (Oberacker et al., 2018). Messenger RNA sequencing libraries were prepared using the Illumina TruSeq Stranded mRNA sample preparation kit (Illumina), as per the manufacturer's instructions. Bisulfite-coverted genomic libraries were prepared using a modified post-bisulfite adapter tagging method.