GSM5292097: t63_Normal; Homo sapiens; RNA-Seq
Fresh lung tumor and matched adjacent non-involved tissues were obtained from patients directly after surgery, flash frozen and stored at -80*C. RNA was harvested using Tryzol reagent. 500ng of RNA were used for libraries construction. Construction of RNA libraries was performed using the QuantSeq Rev 3'mRNA-Seq Library Prep Kit for Illumina (Lexogen, Vienna, Austria). Libraries were pooled and sequenced on the Illumina Hiseq 2500 with 100 bp single end reads.