GSM4726827: AD009D2_S12; Homo sapiens; RNA-Seq
Skin biopsy specimens were collected from the injection site 72 hours after injection with VZV antigen or 6 hour after normal saline injection. Normal (uninjected) skin from the same site was collected as a control. This was done before and after vitamin D supplementation within the same individuals. Frozen tissue was homogenized, and total RNA was extracted from bulk tissue homogenates by using the RNeasy Mini Kit (Qiagen, Hilden, Germany) Kappa Hyperprep kit (Roche Diagnostics)