GSM6322846: AC16, siMLF1, 1; Homo sapiens; ATAC-seq
About 50000 AC16 cells were lysed in ATAC-seq lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2 and 0.5% NP-40) and centrifuged for 10 min at 4°C. Then, nuclei were spun at 1500g for 5 min to remove the supernatant. Nuclei were then incubated with the Tn5 transposome and tagmentation buffer at 37 °C for 30 min using TruePrep® DNA Library Prep Kit V2 for Illumina (Vazyme, TD501-01). After DNA purification with the MinElute kit, and the PCR was performed to amplify the library for 12 cycles and libraries were purified with the MinElute kit. Amplified ATAC libraries were sequenced on the Illumina Novaseq 6000 platform.