GSM6324068: e4.TMAO; Mus musculus; RNA-Seq
The scRNA-seq was performed on fluorescence-activated cell sorting (FACS) enriched live CD45+ cells from tumors of orthotopic PDAC bearing mice. Single cell droplets were generated using the Chromium next gem single cell 3' kit v3.1 (10x Genomics) cDNA synthesis and amplification, library preparation and indexing were done using the 10x Genomics Library Preparation kit (10x Genomics)