GSM5267151: Liver sample 552 - HNKO; Mus musculus; RNA-Seq
RNA was extraced from full liver/cell pellets using trizol, followed by Rneasy mini kits Libraries were constructed followed using the Illumina TruSeq Stranded total RNA Gold protocol. 500 ng RNA pr sample was depleted of rRNA by RiboZero beads, fragmented, and cDNA was synthesized using SuperScript III Reverse Transcriptase (Thermo Fisher Scientific). cDNA was adenylated to prime for adapter ligation and after a clean-up using AMPure beads