GSM4998280: C-1_MeRIP; Homo sapiens; RIP-Seq
50 μg RNA was isolated from human lung tissues using Trizol reagent followed by purification using RiboMinusTM Eukaryote Kit v2 (A15020, Invitrogen). Purified RNA was chemically fragmented into approximately 100 nt using Ambion RNA fragmentation regents and subjected to immunoprecipitation using m6A antibody. library strategy: MeRIP-seq