GSM4877940: RBSS-Sib50; Danio rerio; Bisulfite-Seq
Genomic DNA was extracted from 10 larvae by using a DNA extraction buffer (10mM Tris-HCl pH9, 10mM EDTA, 200mM NaCl, 0.5% DSD, 200µg/ml proteinase K) as previously described (Chernyavskaya et al., 2017). DNA was resuspended in water and quantified by Qubit dsDNA High Sensitivity kit DNA quantitiy and quality was assesed by Qbit. 50-250ng of DNA was used for library prep. Libraries were prepared according to Garrett-Bakelman et al., 2015.