GSM5820815: 5974_4_SKMEL-YAP_R2_TTAGGCA_S41_R1_001.fastq.gz; Homo sapiens; RNA-Seq
Total RNA was isolated from A375-YAP5SA, SKMEL28-YAP5SA and respective EV control cells using Trizol (Thermo Fisher) Strand-specific libraries were generated using the TruSeq Stranded mRNA sample preparation kit (#RS-122-2101/2; Illumina) according to the manufacturer's instructions. The generated cDNA fragments were 3' end adenylated and ligated to Illumina paired-end sequencing adapters and subsequently amplified by 12 cycles of PCR