GSM5225929: ChIP_ESC_wtRes_Kap1_2; Mus musculus; ChIP-Seq
5×10^6 ES cells per reaction were fixed in 1% formaldehyde and neutralized by 125 mM glycine. The crosslinked cells were pelleted, lysed and subjected to sonication. 1/100 of the product was saved as the input, and the rest was mixed with antibody-bound Dynabeads Protein A/G for IP reaction. DNAs of input and IP samples were extracted using phenol chloroform coupled with isopropanol precipitation. DNAs purified from input and IP samples were subjected to library generation using HyperPlus Library Preparation Kit.