GSM5543461: CON CT4-1 (ATAC-seq); Mus musculus; ATAC-seq
50 islets (~50,000 cells) per reaction were  counted, washed with ice cold 1X PBS, and lysed for 20-25 min on ice (10 mM Tris–HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% Igepal CA-630). Nuclei were subsequently obtained following centrifugation for 15min at 500g and 4°C and subjected to the published Omni Assay for Transposase-Accessible Chromatin using sequencing (ATAC-seq) protocol (Corces, M.R. et al., 2017).