GSM5090142: Forelimb, E4; Gallus gallus; RNA-Seq
Forelimbs from 2 different E4 embryos, 3 different E6 embryos and 3 different E10 embryos were dissected and dissociated by collagenase and mechanical treatments. 5000 cells per conditions were loaded into the 10X Chromium Chip with the Single Cell 3' Reagent Kit v3 according to the manufacturer's protocol. Samples were pooled together at each developmental stage (E4 (N=2), E6 (N=3) and E10 (N=3)) before making the libraries. mRNA libraries were prepared for sequencing using standard Illumina protocols