GSM5513613: HUVEC co-culture with wildtype MDF 3; Homo sapiens; RNA-Seq
Total RNA from skin was isolated using Rneasy kit (Qiagen) rRNA removal was performed by Ribominus Eukaryotic system v2 kit (ThermoFisher Scientific). rRNA depleted RNA was subjected to preparation of Illumina compatible RNA-seq libraries using NEBNext Ultra II Directional RNA Library Prep Kit (NEB).