GSM5226271: Naïve_7; Homo sapiens; RNA-Seq
PBMCs isolated from whole blood of dengue patients were sorted for naïve B cells (CD19+ CD20+ IgD+ CD71- CD27- CD38- ) and plasmablasts (CD19+/int CD20- IgD- CD71+ CD27+ CD38+). RNA isolated from sorted cell type of each patient using Qiagen Rneasy micro kit  with on-column DNase digestion. cDNA synthesized from 500 pg of total RNA using Clontech SMART-seq v4 ultra low input RNA kit as per the manufacturer's protocol. Amplified cDNAs were appended with dual indexed barcodes using Nextera XT DNA library preperation kit (Illumina).