GSM5001875: CALM_AF10_Jak1_floxed_R2; Mus musculus; RNA-Seq
AML cells were grown in murine bone marrow medium,pelleted, and washed with PBS before pelleting again. RNA was harvested using Trizol reagent and cleaned up by repurification using the RNEasy Mini Kit (Qiagen) . Illumina TruSeq RNA Sample Prep Kit was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols