GSM6208357: 21-day-old, xrn3-3, replicate 1; Arabidopsis thaliana; RNA-Seq
Total RNA was extracted from the powder of frozen plant tissues by PureLink Plant RNA Reagent (Thermo Fisher) and MaxTract high-density gel tubes (Qiagen). PARE libraries were constructed from 80 μg total RNA following the protocol described previously ( Zhai J, Arikit S, Simon SA, Kingham BF, Meyers BC: Rapid construction of parallel analysis of RNA end (PARE) libraries for Illumina sequencing. Methods 2014, 67:84-90).