GSM5394169: pMEF33_HAT1_KO; Mus musculus; RNA-Seq
Total RNA was extracted from primary mouse embryonic fibroblasts using Total RNA purification plus kit (Norgen Biotek Corp.) according to the manufacturer's instructions. The RNA quality was tested using Agilent 2,100 Bioanalyzer, and RNA libraries were prepared using 200 ng total RNA as per manufacturer's instructions for the TruSeq RNA Sample Prep Kit (v2, Illumina). Concentration and size distribution of completed libraries was determined using an Agilent Bioanalyzer.