GSM5001928: Cortex_Exosc10-3; Mus musculus; RIP-Seq
Exosc10 RIP-seq was performed using Magna RIP™ Kit (merckmillipore) according to the manufacturer's instructions. Dissociated cells from E12.5 cortex on 10 cm dish were lysed in 400 µl of complete RIP lysis buffer-containing protease inhibitors and RNase inhibitor. Exosc10 protein was pulled down by Dynabeads-associated Exosc10 antibody. A mock pull-down was done with normal rabbit IgG (Cell Signaling Technologies). The immunoprecipitated complex was washed intensively and the pulled down RNA was extracted using Trizol reagent.