GSM6323632: Th17 ITA1  ATAC; Mus musculus; ATAC-seq
Cell pellets from T cells in the presence or absecnce ITA on day2  were resuspended in lysis buffer, pelleted, and tagmented using the enzyme and buffer provided in the Nextera Library Prep Kit (Illumina). Tagmented DNA was then purified using the MinElute PCR purification kit (Qiagen). For ATAC sequencing, tagmented DNA was amplified with 10 cycles of PCR, and purified using Agencourt AMPure SPRI beads (Beckman Coulter).  Resulting material was quantified using the KAPA Library Quantification Kit for Illumina platforms (KAPA Biosystems), according to the manufacture's protocol.