GSM5003918: Spinal cord cells - GFAP::EGFP-; Xenopus laevis; RNA-Seq
Spinal cords from animals at R-stage were dissected from the transgenic line Xla.Tg(Dre.gfap:EGFP)Larra. Posteriorly, EGFP+ and EGFP- cells were separated using Fluorescence-Activated Cell Sorting (FACS) and the purification of total RNA from both cell populations was performed using the RNeasy Micro Kit. RNA libraries were prepared by Macrogen (South Korea) using SMTer Universal low amount RNA library kit. Posterioly these were sequenced on the Illumina HiSeq 4000.