GSM6322905: Nudt7-/-  Knockout, Male, Control Diet - 4; Mus musculus; RNA-Seq
RNA was isolated from flash frozen tissue using the TRI Reagent, as per manufacturer's instructions. Following the removal of genomic DNA with Turbo DNA-free kit (Thermo Fisher Scientific), mRNA levels were quantified by RT-PCR using the Quantitect SYBR Green RT-PCR kit (Qiagen). Total RNA was used for library construction using the KAPA mRNA HyperPrep Kit (Roche)