GSM4818751: WNT Dawn Rep 4; Oryza sativa Indica Group; RNA-Seq
Tissue samples were stored at -80C until needed. The leaf tissue samples were first grinded. The tissue then lyophilized at -60C overnight before RNA extractions.Total RNA was extracted using RNeasy Plant Mini Kit (Qiagen) with the recommended RLT lysis buffer. The RNA extraction provided protocol was followed the inclusion of DNase step. RNA concentration was then measured with NANOdrop 2000. 2ug of RNA was used with NEBNext Poly(A) Magnetic mRNA isolation kit (NEB). Oligo(dT) attached to magnetic beads isolate mRNA by attaching to ploy(A) modified mRNA RNA libraries were prepared for sequencing using NEB ultra directional library prep kit