GSM5735839: Mouse Lung ILC Pool 1; Mus musculus; RNA-Seq
Single cell RNA sequencing (scRNA-seq) preprocessing and analysis: Female and male mice were infected with PR8 virus intranasally, and lungs were harvested at day 7 p.i.  Lung ILCs were pooled from 4-5 female or male mice and sorted as lineage-negative (CD3, CD5, TCRβ, B220, CD11b, CD11c, TER119, Gr-1, CD19) CD90+IL-7Rα+ cells using a BD FACS Aria II instrument. Female and male ILCs were tagged with distinct Totalseq anti-mouse Hashtag (1 and 2) mAbs (anti-CD45 and MHCI) (Biolegend) to facilitate separation of the ILCs by sex in the sequencing analysis.