GSM5004112: JB417_CTCF; Homo sapiens; ChIP-Seq
Purified CD4+ T cells were fixed in 1% formaldehyde  in PBS for 10 min at room temperature, followed by 5 min blocking in 125 mM glycine The ChIP assay was carried out using a kit from Diagenode following the manufacturer's protocols. Then DNA-sequencing was conducted using the Illumina HiSeq 2500  platform. The sequencing libraries were generated on the streptavidin beads using the Nextera DNA Library Prep Kit.