GSM4818537: Young 3; Mus musculus; RNA-Seq
RNA was extracted from Trizol reagent with Chloroform and RNA quality was determined (RIN>7) via Agilent Bioanalyzer total RNA was used to generate the RNA sequencing library. cDNA synthesis, end-repair, A-base addition, and ligation of the Illumina indexed adapters were performed according to the TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego, CA). The concentration and size distribution of the completed libraries was determined using an Agilent Bioanalyzer DNA 1000 chip (Santa Clara, CA) and Qubit fluorometry (Invitrogen, Carlsbad, CA).