GSM5605838: IgG; Homo sapiens; OTHER
The beads were washed and CUTANA pAG-Tn5 for CUT&Tag (EpiCypher Inc.) was binded to the secondary antibody. After incubated them with a solution containing Mg2+ to cleave the target DNA, the DNA was extracted and was amplified by 14 cycles of PCR. The PCR product was purified using AMPureXP magnetic beads (Beckman Coulter).Sequence libraries were pooled at 5 nM, and if less than 5 nM (e.g. non-specific IgG), the entire library was pooled. The pooled library was sequenced on HiSeqX (Illumina) using 150bp paired end reads. CUT&Tag