GSM5194388: WGBS - suvh2suvh9+SUVH9(WT); Arabidopsis thaliana; Bisulfite-Seq
Siliques were cut open with insulin syringe needles, whole seeds were transferred to a tube and frozen. DNA was isolated from 250 to 500 young seeds at the mature embryo stage using the Plant/Seed DNA Miniprep kit (Zymo Research). 5ng of DNA was converted and processed into sequencing libraries with the Zymo Pico Methyl-seq Library Prep kit, according to the manufacturer instructions. Libraries were pooled together.