GSM6025859: Vaccinated_unstimulated_VC53; Homo sapiens; RNA-Seq
Total RNA of 3 x 2.5x10^5 macrophages were isolated using the mirVana RNA isolation Kit (ThermoFisher) RNA-Seq library prep was performed with 100 ng total RNA input and the NEBNext Ultra RNA library prep protocol according to standard procedures. Libraries were validated and quantified (Tape Station 4200). All libraries were quantified by using the KAPA Library Quantification Kit and the 7900HT Sequence Detection System. Sequencing was done with NovaSeq6000 sequencers with a PE100bp read length aiming at 50 M reads/sample. Demultiplexing and FastQ file generation was performed using Illumina's bcl2fastq2 software (v2.20.0).