GSM5291233: DRG-Con_rep2; Rattus norvegicus; RNA-Seq
At day 7, rats were deeply anesthetized with sodium pentobarbital (50 mg/kg) and were perfused transcardially with 200 mL 0.9% saline (4℃). After the perfusion, the left (contralateral) side L4-6 DRG segments were collected and were immediately preserved in the RNA later solution (Thermo Fisher, USA). Total RNA was extracted using the Trizol reagent (Thermo Fisher, USA) following the manufacturer's instructions.  DNase I was used to degrade contaminating DNA. The purity and concentration of the samples was assessed by Nanodrop Spectrophotometer (NanoDrop Products, USA) and the RNA integrity was assessed by Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA). RNA libraries were prepared for sequencing using standard Illumina protocols The fragments were enriched by PCR amplification to construct a library ready for sequencing using BGISEQ-500 by BGI Group (Shenzhen, China).