GSM5578344: mES_48h_A (ATACseq); Mus musculus; ATAC-seq
Nuclei from 100,000 live cells for each sample were extracted according to Coerces et al. protocol. Ten PCR cycles were performed for each library using a SimplyAmp (Applied Biosystems, #A24811) thermal cycler. Finally, the libraries were run on a 2% agarose gel using the E-gel electrophoresis system (Thermofisher, #G6400EU) for size selection. Fragments ranging from 200 bp to 700 bp were then purified using the Zymoclean Gel DNA Recovery Kit (Zymo Research, #D4007).