GSM6206936: SEM_CTCF-AIDV2.0_6hr-R1; Homo sapiens; OTHER
Total RNA was extracted from cells in a dark room using Trizol (Thermo Fisher Scientific, 15596026).  3ug RNA was used for thiol modification and then purified using the zymo RNA clean and concentrator kit. The QuantSeq 3' RNA-Seq Library Prep Kit for Illumina (Lexogen) was used for library construction