GSM5194615: Control 4; Mus musculus; RNA-Seq
After cutting the ends of bones and flushing marrow with PBS, the cortical bone was placed in an Eppendorf tube and immediately snap-frozen in liquid nitrogen.  Subsequently, Trizol was added while the bone is maintained frozen in an eppendorf tube placed in a liquid nitrogen bath, homogenized in the same eppendorf tube with the Fastprep24 machine (MP Biomdicals), and subsequently total RNA was isolated and purified using manufacture recommendations for the PureLink RNA kit (Life Technologies). RNA libraries were prepared for sequencing using standard Illumina protocols