GSM6267077: Macrophage, ischemic brain of Foxp3Egfp mice, rep1, scRNAseq; Mus musculus; RNA-Seq
Foxp3Egfp mice were flushed with PBS and single cell suspensions were prepared after flow sorting of CD45+CD11b+CD3-F4/80+ cells using a BD FACSAria cell-sorting system (BD Biosciences, San Jose, CA). Cells were droplet separated using Chromium™ Single Cell 3' v3 Reagent Kit with the 10x Genomics microfluidic system creating cDNA library with individual barcodes for individual cells. Barcoded cDNA transcripts from stroke mice were pooled and sequenced using the novaseq 6000 Sequencing System.