GSM5394009: Cultured_VSMCs_AR004; Mus musculus; RNA-Seq
Cells were washed in PBS, lysed in Trizol (Thermo-Fisher) and total RNA isolated. The extracted RNA was cleaned on a RNeasy column (Qiagen) and quality-assessed on a Bioanalyzer (Agilent, RNA integrity number [RIN] 7.8-9) Sequencing libraries were made from 4 ug total RNA using the TruSeq Stranded mRNA Library Prep Kit (Illumina).