GSM6025930: DRIP - shScr.RNase.rep2; Homo sapiens; ChIP-Seq
RNA pol II (total or pSer2) was immunoprecipitated from sonicated chromatin for ChIP-seq Samples. DNA-RNA hybrid was immunoprecipitated from sonicated chromatin using S9.6 antibody for DRIP-seq Samples. ChIP-seq samples were prepared as outlined by Takara Bio USA Inc's ThruPLEX DNA-seq Kit user guide and DRIP-seq samples were prepared and sequenced by Novogene using their ChIP-seq protocols (NEB Next Ultra II DNA Library preparatino kit).