GSM5856153: E12.5_CM_p300_RNA-seq_rep3; Mus musculus; RNA-Seq
For E12.5, E16.5, P0, and P7 CMs, ventricular apexes were dissociated using the Neonatal mouse cardiomyocyte isolation kit (Cellutron Life Technologies) and purified using the mouse neonatal cardiomyocyte isolation kit (Miltenyi). P14, P28, and P42 CMs were dissociated and purified from ventricular apexes by Langendorf collagenase perfusion followed by differential sedimentation. Purified cardiomyocytes were used with three biological replicates per group for RNA-seq. RNA was purified using the Purelink RNA mini kit (Life Technologies). Ribosomal RNA was depleted using Ribo-Zero rRNA removal kits (Epicentre). RNA-seq libraries were prepared using ScriptSeq v2 library kit (Epicentre). Libraries were sequenced using an Illumina NextSeq500 with single end 75   bp reads. At least 50 M reads were obtained for each biological replicate.