GSM5291605: THESC + DMSO rep1; Homo sapiens; RNA-Seq
After incubation, cells were lysed and RNA harvested using TRIzol Reagent (Ambion, Life Technologies). An additional step was performed to remove excess DNA with the TURBO DNA-free kit (Invitrogen) according to manufacturer's recommendations except that a longer centrifugation step (3 min) was carried out. RNA libraries were prepared for sequencing using standard Illumina protocols