GSM6327115: S2_WT3_GFP_RNAi_mRNA; Drosophila melanogaster; RNA-Seq
RNA was extracted with TRI Reagent (Life Technologies), according to the manufacturer's protocol. Briefly, samples were homogenized in TRI reagent, and RNA was phase-separated by the addition of chloroform (J.T. Baker Analytical) at a ratio of 250 µL per 1 mL TRI Reagent. RNA was then precipitated with isopropanol, with GlycoBlue (Life Technologies) added as a co-precipitant when necessary, and pellets were washed twice with 70% ethanol prior to resuspension in water. RNAseq libraries were prepared with the Kapa RNA Hyperprep Kit with mRNA capture (Roche diagnostics). These libraries were sequenced on an Illumina NovaSeq platform with 50-nt paired-end reads