GSM5514047: MPP3_ERlow_rep2; Mus musculus; RNA-Seq
For bulk RNA-seq, cells (5,000 to 7,000 per sample) were directly sorted into 350 μl RLT lysis buffer containing 1% b-mercaptoethanol from the RNeasy Plus Micro Kit (Qiagen, 74034) and RNA was isolated according to the manufacturer's instructions. For bulk RNA-seq, libraries were prepared using Trio RNA-seq Library Preparation Kit (NuGen, 0507) and for single cell RNA-seq, libraries were prepared using a Chromium Single Cell 3′ Library & Gel Bead Kit v2 (10X Genomics).