GSM5291821: KM-1; Mus musculus; RNA-Seq
Single cells were suspended in calcium- and magnesium-free PBS containing 0.04% weight/volume BSA.Cells were resuspended to a final cell concentration of 700~1200 cells/μl with more than 85% viability. 8,000-12,000 cells were captured in droplets. After the reverse transcription step, emulsions were broken and barcoded-cDNA was purified with Dynabeads, followed by PCR amplification (at 98°C for 45 sec; at 98°C for 20 sec, 67°C for 30 sec, 72°C for 1 min with 13-18 cycles; at 72°C for 1 min). Amplified cDNA was then used for 3' gene expression library construction. RNA libraries were prepared with Chromium Single cell v2 or v3 Kits according to the manufacturer's protocol