GSM5732547: Input_2h1_TRB1-GFP; Arabidopsis thaliana; ChIP-Seq
anti-GFP ChIP were performed as follow: Chromatin was extracted and resuspended in 300 µL Nuclei Lysis Buffer, the samples were flash frozen in liquid nitrogen to help breaking the nuclei. Chromatin was sheared using a Diageode Bioruptor to obtain fragments between 75 and 300 bp. ChIP-Seq NEBNEXT Ultra II kit