GSM5548445: Cold BMKO rep1; Mus musculus; RNA-Seq
Total RNA was extracted using Trizol followed by DNase digestion and Qiagen RNeasy column purification. Sequencing libraries were prepared with Kapa RNA mRNA HyperPrep kit according to the manufacturer's protocol, and sequenced on Illumina HiSeq 2500 with SR V4 Kit with the single read mode of 51cycle of read1 and 7 cycles of index read.