GSM5393702: Zcchc8_KO2_MPP8_ChIPseq; Mus musculus; ChIP-Seq
Cells were crosslinked with 1% formaldehyde for 10 minutes at RT before quenching with glycine. DNA was sheared to ~ 200 bp using a Bioruptor (Diagenode). ChIPs were carried out using using 300 µg of chromatin and 5 µg of MPP8 antibody (Proteintech, 16796-1-AP) ChIPseq libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (NEB) using AmpureXP beads (Beckman) for size selection.