NextSeq 500 sequencing; Meis homeobox genes control cell competence within the eye-specified neuroepithelium (RNA-seq)
The retinal progenitor cells from mRx-Cre;Meis1f/f;Meis2f/f;R26-EYFP (Meis1/2cKO) and mRx-Cre;R-26EYFP (WT) retinae were isolated using FACS. RNA was isolated by Qiagen RNeasy Micro (Qiagen). RNA-seq libraries were prepared using NEBNext Ultra II Directional RNA Library Prep kit (NEB, E7760L).