GSM4878386: E18.5 (Bmx-CreER Wls): HSPC+microenvironmental cells;; Mus musculus; RNA-Seq
Bone marrow from corresponding time point is crushed by pestle and collected for lysis by collagenase type 4. The cell lysate is filtered to obtain single cell suspension solution. Lineage committed hematopoietic cells are magnetically depleted by MACS separation protocol.  The remaining Lin- cells, which contain HSPC and microenvironemental cells, are used for scRNA-seq library preparation and sequencing Single cell whole transcriptome analysis libraries were prepared according to the manufacturer's instructions using BD Rhapsody WTA Reagent kit (BD)