GSM6030606: Kidney Wnt4-lineage cells, Brg1-deficiency, scRNA-Seq; Mus musculus; RNA-Seq
Individually barcoded scRNA-seq libraries were prepared using the Chromium Platform (10x Genomics) with the 3' gene expression V3 kit, using an input of approximately 10,000 cells. Briefly, Gel-bead in emulsions (GEM) were generated on the sample chip in the Chromium controller. Barcoded cDNA was extracted from the GEMs by post-GEM RT-cleanup and amplified for 12 cycles. Amplified cDNA was fragmented and subjected to endrepair, poly A-tailing, adapter ligation, and 10x-specific sample indexing following the manufacturer's protocol. Libraries were quantified using Bioanalyzer (Agilent Technologies) and QuBit (Thermo Fisher Scientific) analysis.