GSM5820730: HC11; Homo sapiens; RNA-Seq
Single cell suspension, barcoded Gel Beads and partitioning oil were loaded to Chromium Chip G to generate single-cell gel bead-in-emulsion (GEM). The poly-adenylated transcripts were reverse-transcribed later. The downstream library constructions were performed using the Chromium Single Cell 5' library construction kit according to the manufacturer's protocol (10x Genomics).