GSM5226899: H-CLP48H-5; Mus musculus; RNA-Seq
After collected, the hearts were put in liquid nitrogen quickly, and preserved in low temperature environment. TRIzol (Invitrogen, Waltham, Massachusetts, USA) was used to isolate total RNA from heart tissues following a manufacturer's protocol. Total RNA quantity was determined with Nanodrop 2000 (Thermo Scientific, Waltham, Massachusetts, USA) and quality was assessed by running an 1% agarose gel electrophoresis, stained with 4S Red Plus Nucleic Acid Stain (Sango, Shanghai, China). RNA-seq libraries were prepared using the KAPA Stranded RNA-Seq Library Preparation Kit.