GSM5457170: shVprBP_rep3; Homo sapiens; RNA-Seq
RNA was prepared from SW620 cells using the Qiagen RNeasy kit (Qiagen Inc., CA, USA) according to the manufacturer's instructions Strand specific libraries were prepared using a KAPA Stranded mRNA-Seq Kit with KAPA mRNA Capture Beads (KAPA Biosystem Inc., MA, USA), and validated on an Agilent Bioanalyzer with the DNA1000 kit. Pooled libraries were prepared, denatured, diluted to 15 pM, and then clonally clustered onto the sequencing flow cell using the Illumina cBOT Cluster Generation Station and Cluster Kit v3-cBot-HS. The clustered flow cell was sequenced with 1X50 SE reads on the Illumina HiSeq2000 according to manufacturer's protocol. Base conversion was made using OLB version 1.9, de-multiplexed, and converted to Fastq using CASAVA version 1.8 (Illumina, CA, USA).