GSM5225899: E9.5 P-Sp V+61+E+ sort; Mus musculus; RNA-Seq
FACS-isolated primary cells were washed with PBS/0.04% ultrapure BSA, and resuspended in PBS/BSA for loading into the 10X Genomics Chromium Single Cell B Chip, and processed in the Chromium single cell controller The 10X Genomics Version 2 single cell 3' kit was used to prepare single cell mRNA libraries with the Chromium i7 Multiplex Kit, according to manufacturer protocols.  Sequencing was performed for pooled libraries from each sample on an Illumina NextSeq 500 using the 75 cycle, high output kit