GSM5620673: UNx db/db LacZAAV 8; Mus musculus; RNA-Seq
RNA from snap-frozen kidney cortex samples (∼15 mg per animal) stored at −80°C was extracted using a NucleoSpin 8 RNA kit (MachereyNagel) and a vacuum manifold cDNA library preparation was performed using an NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760L, NEG, Ipswich, MA, USA). The number of cycles used for cDNA amplification was 16. The sequencing of cDNA libraries was performed using an NS 500 high Output Kit v2 (75 cycles) (Illumina, San Diego, CA, USA) on an Illumina NextSeq 500 platform (Illumina, San Diego, CA, USA).