GSM5460615: E3.5_MZ KO [C61_2]; Mus musculus; Hi-C
single embryo HiC-Seq and single embryo RNAseq Single cell Hi-C protocol was adapted from Nagano et al., 2015, optimizing it for single embryo by scaling the reaction volumes and reducing the experimental procedures to avoid sample loss. For single embryo RNAseq, CDNA synthesis was performed using SMART-Seq Ultra Low Input RNA Kit (Clontech) and Sequencing was performed using the Illumina HiSeq 2500.