GSM6027313: AIS22; Homo sapiens; RNA-Seq
EVs were purified from 0.5-1 mL serum using the exoRNeasy Midi kit (Qiagen; Cat#77144) according to the manufacturer's instructions. For RNA extraction, EVs were lysed on column with 1 mL Trizol reagent (Invitrogen; Cat#15596026) and the RNA pellet was dissolved in 11 μL of deionized water treated with DEPC (diethylpyrocarbonate). EV long RNA libraries were constructed using poly(A) independent extracellular RNA sequencing . Briefly, 1 ng RNA was reverse transcribed with random primer by SMART technology. cDNA was purified twice using Ampure XP beads (Beckman Coulter, Cat# A63881) and amplified with indexed primers. 200–500 base-paired PCR products were size-selected using Ampure XP beads.