GSM5578574: TBI-4; Homo sapiens; RNA-Seq
Biopsy of abdominal subcutaneous adipose was performed under local anesthesia through a small incision in the lower abdomen followed by aspiration of 2-3 g of tissue with a 4 mm blunt-end liposuction needle. Tissue was flash-frozen. RNA was extracted from frozen adipose tissue with Trizol (Invitrogen) and purified with RNAeasy columns (Qiagen). RNA libraries were prepared for sequencing using standard Illumina protocols