GSM5092471: mSSCs, Injured Rep 1; Mus musculus; RNA-Seq
Achilles Tendon-To-Bone Interfaces were removed and digested under fluorescence-activated cell sorting  for mSSCs. RNA extraction was performed using Qiagen miRNeasy kit (cat. 1071023)  with one column DNAse treatment per the manufacturer's recommendations. To generate cDNA from 150 pg total RNA, the Clontech Smarter Ultra Low Input RNA kit (Takara Bio, Cat. 634848) was used per the manufacturer's recommendations. Amplified cDNA was purified using SPRI Ampure Beads (Beckman Coulter, Cat. A63880) and the quality and quantity was measured using a High Sensitivity DNA chip on the Agilent 2100 Bioanalyzer (Agilent Technologies). RNA libraries were prepared for sequencing using standard Illumina protocols