GSM5348501: Yb_EC10_1; Saccharomyces cerevisiae; RNA-Seq
Cells were harvested by centrifugation at 4000 rpm for 1 min at room temperature and snap frozen. After cell disruption by bead-beating, total RNAs were column purified using the RNeasy mini kit (Qiagen) according to the manufacturer's instructions, and gDNA was removed using DNAse I (Sigma Aldrich). RNA integrity was further assessed using a Bioanalyzer 2100 (Agilent, CA, USA). Strand-specific cDNA libraries were prepared using the TruSeq-3 library kit