GSM5394446: Adipo+NAD+,Day16, R1; Homo sapiens; RNA-Seq
Total RNA from MSCs was extracted using Quick RNA MiniPrep Kit (Zymo Research, USA) following the manufacturer´s instructions RNA samples with RNA integrity number (RINs) > 7.0 were sent for library preparation and sequencing to Novogene Corporation Inc., California, USA. Briefly, mRNA was isolated using oligo(dT) beads and randomly fragmented by adding fragmentation buffer, followed by cDNA synthesis primed with random hexamers. Next, a second-strand synthesis buffer (Illumina), dNTPs, RNase H and DNA polymerase I were added for second-strand synthesis. After end repair, barcode ligation and sequencing adaptor ligation, the double-stranded cDNA library was completed with size selection to 250-300 bp, and PCR enrichment