NextSeq 500 sequencing; ChIP sequencing on human, circulating, ex vivo isolated Naive CD4+ T cells
CD4+ naive T cells have been isolated from peripheral PBMCs by density gradient centrifugation with Ficoll-Paque Plus. From PBMCs, T cells were negatively selected with magnetic separator (AutoMACS). CD4+ Naive were FACS sorted as CD4+CD25-CD127-/highCD45RO-. CD4+ Naive T cells were treated with control (scr) or LINE1 ASO. Cross-linked, sonicated and Immunoprecipitated DNA was purified with AMPure beads XP NEB Next Ultra II