GSM5087812: NC-2; Gallus gallus; RNA-Seq
For the extraction of total RNA from all collected caecum samples, a modified Trizol method was used and the RNA was extracted according to the instructions of Trizol kit manual (B518651-0100, Sangon Biotech co, Ltd, Shanghai, China). The purity of RNA was determined using NanoPhotometer® Spectrophotometer (IMPLEN, CA, USA) and the concentration of total RNA was determined with Qubit® 2.0 Fluorimeter by using Qubit® RNA Assay Kit (Life Technologies, CA, USA). RNA purity and integrity were further measured with Bioanalyzer 2100 system using RNA Nano Assay Kit (Agilent Technologies, CA, USA). The RNA samples those passed the concentration, purity, and integrity tests without any degradation and contamination were used for transcriptome sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols.