GSM5087744: RBG33508_F90_F103_F107_protein; Homo sapiens; OTHER
Samples were extracted and MNCs isolated using ficoll. A detailed protocol of cell processing, CD34+ enrichment, TotalSeq-A staining and FACs sorting can be found in Simultaneous analysis of single-cell transcriptomes and cell surface protein expression of human haematopoietic and stem cells using the 10x Genomics platform. Sequencing libraries were prepared following 10x Genomics manufacturer's protocol and Biolegend TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3' Reagent Kit v3 or v3.1 Protocol. 10x Single Cell 3′ Kit v3.1 + surface protein (TotalSeq-A).