GSM5580030: drna pKT-CNP5; Salmonella enterica subsp. enterica serovar Typhimurium; RNA-Seq
RNA was isolated using the RNAsnap method, treated with Dnase I, and RNA quality checked as described in Kurasz et al. 2018. J. Bacteriol. 200(23):e00476-18. Extracted RNA was sent on dry ice to the Microbial Genome Sequencing Center (Pittsburgh, PA, USA) where all following steps, incuding data processing, were completed. 300 ng RNA for each sample were subjected to ribosomal RNA depletion using the Ribo-Zero™ Plus rRNA Removal Kit and complementary DNA (cDNA) libraries were then prepared with the Illumina Stranded RNA library preparation kit.