GSM5580117: Chsol; Homo sapiens; RNA-Seq
7 µm sections of fresh frozen tumor tissue were stained according to the protocol preserving RNA, the microenvironment of morphological structures was isolated using laser caption microdissection. RNA was extracted from the microdissected samples using Single Cell RNA Purification Kit (Norgen, Thorold, ON, Canada). RNA samples were immediately used to generate cDNA libraries using SMARTer Stranded Total RNA-Seq Kit v2 — Pico Input Mammalian (Takara, Mountain View, CA, USA). RNA libraries were prepared for sequencing using standard Illumina protocols