GSM6164985: MDM cells of HC2 patient not stimulated; Homo sapiens; RNA-Seq
Total RNA was extracted from primary fibroblasts from patients or healthy controls with the the miRNeasy Mini Kit (Zimo), and treated with DNAse (Zymo) to remove residual genomic DNA. Quality controls were performed and all RNA samples were of good quality (RIN>8). RNA-Seq libraries were prepared with the Illumina RiboZero TruSeq Stranded Total RNA Library Prep Kit (Illumina) and sequenced on the Illumina NextSeq platform in the 150-nt (for monocyte-derived and iPSC-derived macrophages) or 100-nt (for primary fibroblasts), paired-end configuration.