GSM4818168: Flower_H3K9me2_ChIPseq; Arabidopsis thaliana; ChIP-Seq
For H3K9me2 ChIP-seq in Arabidopsis flowers, 3 grams of Arabidopsis Col-0 wild-type unopened flower buds were collected. The nuclei were isolated from these materials for in vitro cross-linking with 1% formaldehyde. Nuclei were lysed and the chromatin was sheared with Bioruptor Plus (Diagenode). The sheared chromatins were equally separated for two ChIPs. 5 μl of anti-H3K9me2 (ab1220, abcam) and anti-H3 (ab1791, abcam) antibodies were added for chromatin immunoprecipitation, respectively. This experiment was performed by closely following the protocol described in a previous paper (Zhong et al., 2012). ChIP-seq libraries were prepared from DNA extracted from the ChIP experiment using Ovation Ultra Low System V2 Kit following manufacturer instructions (NuGEN). The libraries were sequenced on a HiSeq 4000 sequencer (Illumina) to obtain single-end 50 bp reads.