GSM4726974: MEF_siCtrl_Async_repl3_ATAC-seq; Mus musculus; ATAC-seq
Nuclei were prepared from 50,000 cells and subjected to tagmentation reaction. DNA was extracted and PCR amplified using Nextera Index primers (FC-121-1011) for paired-end sequencing.    DNA was size selected and using Ampure XP magnetic beads. Libraries were quantified using KAPA SYBR FAST qPCR Master mix (KK4932) to determine the optimal number of PCR cycles. After running the remaining number of PCR cycles, libraries were purified with Ampure XP beads (with size slection). Libraries were quantified using the Qubit DNA HS Kit, and for quality control, 1 μl of each sample was run on Bioanalyzer High Sensitivity DNA Chip. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (FC-121-1030, FC-121-1011). In all, 2 nM of all libraries were pooled and 1.5 pM were analyzed on Illumina NextSeq500 (500/550 High Output v2 Kit—150 cycles).