GSM5512935: panx1b knock-out#1; Danio rerio; RNA-Seq
Total RNAs were extracted from 6dpf larvae using RNeasy Plus Mini Kit and automated Qiacube platform (Qiagen). The RNA library preparation was performed following the NEB NEBNext Ultra II Directional RNA Library Preparation protocol (New England Biolabs Inc., Ipswich, MA, USA). RNA libraries were loaded on a Bioanalyzer 2100 DNA High Sensitivity chip (Agilent Technologies) to check for size, quantified by qPCR using the Kapa Library Quantification Illumina/ABI Prism Kit protocol (KAPA Biosystems, Wilmington, MA, USA).