GSM5582260: Ad-Tox4 sh 3; Mus musculus; RNA-Seq
9 days post viral injection, we fasted mice for 16-hr. Then we sac mice and liver tissues were frozen with liquid nitrogen. Liver total RNA were exacted with Trizol reagent and further purified by QIAGEN RNA mini kit. We use poly-A pull-down to enrich mRNAs from total RNA samples,then proceed with library construction using Illumina TruSeq chemistry. Libraries are then sequenced using Illumina NovaSeq 6000 at Columbia Genome Center. We multiplex samples in each lane, which yields targeted number of paired-end 100bp reads for each sample.