GSM5089811: DMSO2_24_5; Rattus norvegicus; RNA-Seq
Cells were lysed with Trizol, mized with chloroform and spun in phase-lock gel tubes inside a cold room. The upper phase was decanted into new tubes and isopropanol and glycogen were added to the mixture which was incubated at -20C overnight. DNA was removed using the TURBO DNA-free kit (Invitrogen, #!M1907). RNA was sent to GeneWiz (https://www.genewiz.com/en) for PolyA selection, library construction, and sequencing