GSM5579734: Snr1_AID_Auxin_2_Mnaseseq; Drosophila melanogaster; MNase-Seq
Cells were washed and permeabilized with .75% NP-40 solution, and Mnase digest on nuclei was performed for 25 minutes at 37C. The DNA was size selected on a gel for mono-nucleosomal fragments. Purified DNA fragments were cloned using NEBNext  Ultra II DNA Library Prep Kit