GSM4998741: Olive flounder with bold personality (BP), replicate 3; Paralichthys olivaceus; RNA-Seq
Total RNA was extracted from each sample using Trizol Reagent according to the manufacturer's protocol, and was treated with DNaseI to eliminate potential genomic DNA contamination. The concentration and integrity were were determined by spectrophotometry (NanoDrop ND-1000 system, Thermo Fisher Scientific, DE, USA)) and gel electrophoresis. Equal amounts of RNA from the hindbrain were obtained from six individuals. The Illumina TruSeq mRNA Stranded Sample Preparation kit was used in constructing the Illumina sequencing libraries according to previous methods (Zhang et al., 2017). The libraries were then sequenced on Illumina HiSeq 2000 using a 100-bp paired-end sequencing module.