GSM5856130: Lung 2; Mus musculus; RNA-Seq
Lung DC and AM were flowcytometry sorted from animals subjected to either control, asthma or tolerization protocol. The cells were labled with HTOs according to treatment and stained with tag CLEC12A. Equal Number of cells were pooled . Then they were processed into single cells and were barcoded using 10x Chromium platform.  Barcoded RNA was extracted using  Chromium Single Cell 3ʹ Reagent Kits v3 User Guide. RNA libraries were prepared for sequencing using standard Illumina protocols.