GSM6267358: LCA1.D150.NoTreat.3; Homo sapiens; RNA-Seq
Total RNA was purified from homogenized retinal organoids using TriPure isolation reagent (Roche). Quality of isolated RNAs was assessed using Bioanalyzer RNA 6000 nano assays (Agilent) and high-quality total RNA (RNA integrity number ≥7.5) were used for construction of mRNA sequencing library. 100ng total RNA were used to construct the strand-specific libraries using TruSeq RNA Sample Prep Kit-v2 (Illumina). RNAseq data were generated using TruSeq Stranded mRNA Sample Prep Kit (Illumina).