GSM5620957: Cardiac cells, STDP, rep1; Mus musculus; RNA-Seq
Total RNA from heart tissue was extracted with the TRIzolTM Reagent (Thermo Fisher Scientific, MA, USA), and the purity and integrity of RNA were detected by agarose gel electrophoresis. Second, sequencing libraries were generated using the Illumina NEBNext® UltraTM RNA Library Prep Kit (NEB, USA) according to the protocols recommended by the manufacturer. Briefly, the mRNA was randomly interrupted and reverse transcription was performed to synthesize double-stranded cDNA, which was purified (~200bp) by the AMPure XP beads after ligation of adaptors to obtain the sequencing library. Third, the content of DNA was detected by a Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA), and the quality of the library DNA integrity was assessed using an Agilent 2100 Bioanalyzer (CA, USA). Last, the qualified samples were sequenced on an illumine HiSeq X Ten platform by Novogene (Beijing, China).