GSM5394387: HSC3_siDLEU1_H3K4me3; Homo sapiens; ChIP-Seq
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Library construction was performed according to the manufacturer's instructions. Briefly, input DNA or the immunoprecipitate was ligated to adapters and PCR amplified using a Ion Plus Fragment Library Kit (Thermo Fisher Scientific). Template preparation and chip loading were carried out using a Ion Chef System (Thermo Fisher Scientific).