GSM4878382: E14 Dnmt3a/b dKO.B 2iLIF RRBS rep2; Mus musculus; Bisulfite-Seq
500ng of DNA was digested at 37°C with 200U of MspI restriction endonuclease (NEB). Digested DNA was than end repaired, dA-tailed, and ligated to methylated adapters, using the Illumina TruSeq DNA Sample Prep Kit, following manufacturer's instructions.