GSM5733026: H007; Mus musculus; RNA-Seq
The hearts were isolated and digested. Cells were stained with relative antibodies and dyes. After incubation, live nucleated CD45+ and CD45- cells were sorted and collected. Sorted single-cell suspensions were loaded to 10× Chromium to capture no more than 10000 single cells according to the manufacturer's instructions of Chromium Single Cell 3' Reagent Kits v3 (10× Genomics). Libraries were sequenced on a Novaseq 6000 (Illumina) .