GSM5512819: ATAC.07_CTCFWAPL_0h; Mus musculus; ATAC-seq
ATAC-seq libraries were prepared following a published protocol (Liu et al. 2017). 100 k mouse embryonic stem cells are harvested. Nuclei were isolated, permeabilized, and tagmented using in-house produced Tn5 transposase. The resulted DNA fragments underwent two sequential 9-cycle PCR amplification and the fragments < 700 bp were purified using SPRI beads (Liu et al., 2017).