GSM6321940: 5; Homo sapiens; RNA-Seq
After PBMC isolation, total RNA in samples were obtained by using TRIzol reagent, and the purity and concentration of them were determined with the instrument of NanoDrop ND-1000. If the OD was in the range of 1.8-2.0, the RNA was included in the follow-up experiment. According to rtStar tRF & tiRNA Pretreatment Kit protocols (Arraystar, USA), the total RNA samples were pretreated to remove RNA modifications Sequencing libraries are size-selected for the RNA biotypes to be sequenced using an automated gel cutter.  The libraries are qualified and absolutely quantified using Agilent BioAnalyzer 2100.