GSM5089693: Notch1-/- ES cells, d0, rep1; Mus musculus; ncRNA-Seq
Total RNA was isolated using Trizol (Invitrogen). RNA quantification and quality control were performed using a NanoDrop ND-1000 and Agilent 2100 Bioanalyzer, respectively Sequencing libraries were prepared according to Illumina long RNA Seq library kit instructions with Poly(A) selection. Libraries were sequenced with the Illumina HiSeq2000 (2x100bp).