GSM5579616: Smc3_HSPCs_2; Mus musculus; RNA-Seq
Total RNA was isolated from 1x10^6 cells using Trizol (ThermoFisher Scientific, 15596018) and the manufacturer's recommended protocol. Libraries were prepped in exact accordance with the NEBNext Ultra II RNA library prep kit for Illumina (NEB, E7770) in combination with the NEBNext Poly(A) mRNA magnetic isolation module (NEB, E7490), the NEBNext multiplex oligos for Illumina set 1 and set 2 (NEB, E7335 and E7500), and Ampure XP magnetic beads (Beckman Coulter, A6388).