GSM5087879: M/24y donor, normoxia, static Compression; Homo sapiens; RNA-Seq
RNA was extracted through chloroform phase-separation and isopropanol precipitation. RNA was purified using QIAGEN RNeasy mini kits with a DNAse digestion step. RNA concentrations were measured using a NanoDrop One. Equal masses of RNA from 3-4 replicate tissues from each donor in each condition were then pooled together to reduce inter-replicate variability (18 pools in total). These samples were then shipped to the UBC Biomedical Research Center for RNA sequencing. Illumina NextSeq 500: PolyA selection, 20million paired reads