GSM5348577: HYMIIGC3; Homo sapiens; RNA-Seq
hGCs were isolated from donors under 28 (21-28) [HY] or over 38 (38-42) years old [HO]. The hGCs were washed with 500 μL phosphate buffered saline (PBS) (Gibco) and centrifuged at 3,000 rpm for 5 min. Then 50 μL hyaluronidase (Sigma) was added to digest hGCs at 37oC for 5 min. The cell suspensions were washed with 100 μL PBS and then 20 μL PBS was added to resuspend cell pellet. RNA-seq libraries were constructed as previously described (Liu et al., 2021). Discover-scTM WTA Kit V2 (Vazyme, N711) was used to obtain cDNA from single oocyte and GCs. The TruePrepTM DNA Library Prep Kit V2 for Illumina (Vazyme, TD503) was used for the purpose of constructing libraries. An Illumina HiSeq X platform (Shanghai, China) performs libraries sequencing. High-quality reads were aligned to Mus musculus UCSC mm9 references and FPKM value of each gene was calculated.