GSM5733583: 170kb Enhancers + SynHoxA assemblon in HoxA -/- mESCs, 48h of RA differentiation, Hi-C; Mus musculus; Hi-C
Cells were collected at 0h and 48h after RA treatment. Cells were divided into ~1x106 aliquots and crosslinked in a final concentration of 2% FA (vol/vol) in 1 × PBS for 10 min at room temperature. After quenching with Glycine, cells incubated on ice for 15 min, pelleted by centrifugation at 500 g, and frozen at -80°C. Hi-C was performed using the Arima-HiC workflow (Arima Genomics, San Diego, CA) by NYU Langone's Genome Technology Center (RRID: SCR_017929).