GSM5348397: KMR19; Mus musculus; RNA-Seq
RNA was isolated using Total RNA isolation kit and post isolation DNAase (Thermo Fisher Scientific)- 2µg were processed Library preparation was performed with the Illumina TruSeq Stranded mRNA Sample Preparation Kit, according to the manufacturers protocol. Denaturation of RNA was performed at 65oC in a thermocycler and cooled down to 4oC. Samples were indexed to allow for multiplexing. Sequencing libraries were quantified using the Qubit fluorometer (Thermo Fisher Scientific, Massachusetts, USA). Library quality and size range was assessed using the Bioanalyzer (Agilent Technologies) with the DNA 1000 kit (Agilent Technologies, California, USA) according to the manufacturer's instructions. Libraries were diluted to a final concentration of 2nM and sequenced on Illumina HiSeq4000 sequencing system, according to the manufacturer's recommendations.