GSM4726442: B6 validation 30 Blood BBA-seq; Mus musculus; OTHER
Genomic DNA was isolated from peripheral blood with the QIAamp DNA Mini Kit (Qiagen),or from buccal swab with NucleoSpin Tissue Kit (Macherey-Nagel). Then was bisulfite converted by the EZ DNA Methylation Kit (Zymo) 10 pM DNA library was diluted with 15% PhiX spike-in control and eventually subjected to 250 bp pair-end sequencing on a MiSeq lane (Illunima, CA, USA) using Miseq reagent V2 Nano kit (Illumina).