GSM5580242: WT-Liver; Mesocricetus auratus; RNA-Seq
RNA was extracted using TRIzol (Invitrogen). For cortex and SCN, tissues from 4 samples of the same genotype were mixed before library construction. Liver tissues from each individual hamster were used for library construction. In total, 12 libraries were constructed for the experiment. 8 libraries were prepared (4 for wild type, 4 for duper). In total, 12 libraries were pooled and subjected to 2×100bp sequencing in two lanes on a HiSeq 2000 sequencer (Illumina). TruSeq Stranded mRNA Sample Preparation Kit (Illumina)