GSM5961444: Retina WT rep3 [WT-c3]; Mus musculus; RNA-Seq
Retinas were dissected in ice-cold PBS and embryonic hylaoid blood vessels were removed. Retinas were immediately transfered to 1 ml of RNA STAT-60 and the retinal tissue was homogenized using 26 guage needle syringe and RNA was harvested using as recommended by the manufacturer (amsbio, LLC). As recommended by the manufacturer (New England BioLabs, Inc) poly (A) mRNA was isolated (using NEBNext®) and 500ng of RNA was used for construction of sequencing libraries (using NEBNext ultra II directional library prep kit for Illumina). RNA libraries were prepared for sequencing using standard Illumina protocols