GSM5598596: 3D Non-balding dermal papilla cell_replicate B; Homo sapiens; RNA-Seq
Total RNA from BAB and BAN samples was isolated with RNeasy Mini kit and on-column DNase treatment. RNA samples of high integrity (RIN value ≥ 8) were used for RNA-seq library construction. RNA-seq library construction and downstream analysis were carried out on technical triplicates. cDNA was generated from 30 ng of extracted RNA with SMARTer Ultra Low Input RNA for Illumina Sequencing (Cat no.: 634823, Clontech), followed by RNA-seq library construction with NEBNext DNA Library Prep Master Mix Set for Illumina (Cat no.: E6040, NEB). Constructed libraries were paired-end sequenced with Illumina HiSeq High Output v3.