GSM4818066: E14_WT_WGBS; Mus musculus; Bisulfite-Seq
Genomic DNA (500 ng) with 1% lambda DNA spike-in was sheared by Bioruptor sonicator to an average size of 350 bp in length. DNA was then end-repaired, dA-tailed, and ligated with methylated adaptors according to the manufacturer's instruction (NEBNext Ultra DNA Library Prep Kit for Illumina #E7645S). Bisulfite conversion was carried out using an EpiTect fast bisulfite conversion kit (Qiagen) according to the manufacturer's instructions. Twenty minutes were used in both conversion steps. Bisulfite-converted DNA was then amplified by PCR to obtain the final library. The WGBS libraries were subjected to pair-end (150 bp) sequencing on a Novaseq instrument (Illumina).