GSM4998163: RNAseq_293T-WT_Rep2_R1; Homo sapiens; RNA-Seq
For RNA-seq, total RNA was extracted using miRNeasy Mini Kit (Qiagen #217004) and quantified using RNA 6000 Nano Kit (Agilent Technologies) using Bioanalyzer. One microgram of total RNA was subjected to the depletion of ribosomal RNA (Ribo-Zero rRNA removal kit ,Illumina). Ribo-depleted RNA-seq libraries were prepared using the TruSeq Stranded Total RNA Library Prep Gold (Illumina) following manufacturer's instructions. Libraries were checked for quality and quantity using Bioanalyzer. Equimolar concentrations of libraries were pooled, requantified and sequenced as 100 bp paired-end reads on the Illumina HiSeq 3000 instrument.