GSM4726232: EpCAMhi MDA-MB-231 Negative siRNA 3; Homo sapiens; RNA-Seq
Total RNA was extracted from each sample using TRIzol and Direct-zol RNA miniprep kit. The RNA integrity number (RIN) of all samples was determined by an Agilent 2100 Bioanalyzer in the Harvard Medical School Biopolymers Facility. All RNA samples had RINs greater than 9. Standard mRNA libraries were prepared with NEBNext® Ultra™ II Directional RNA Library Prep Kit after polyA-mRNA isolation (New England BioLabs). Libraries for negative control and UPF2 siRNA transfected samples were pooled separately and each pool was run on one lane of an Illumina Hiseq X10 system, yielding around 120 million mapped paired-end reads (150bp X 2) per sample.