GSM5090071: MeRIP-seq-sh-NC-INPUT-rep1; Homo sapiens; RIP-Seq
RNA was harvested using Trizol reagent. Seq-StarTM poly(A) mRNA Isolation Kit (Arraystar, Rockville, USA) was used to obtain complete mRNA.1 ug of total RNA for the construction of sequencing libraries in RNA-seq. After fragmentating, RNA was incubated with m6A antibody (ABE572, Merck Millipore, Germany) for immunoprecipitation based on the instructions of the MeRIP m6A Kit (Merck Millipore, Germany).  60 ug of total RNA for the construction of sequencing libraries in MeRIP-seq RNA libraries were prepared for sequencing using standard Illumina protocols