GSM4726526: S45 anti-IgM 72h donorF; Homo sapiens; RNA-Seq
The total RNA was isolated using the RNeasy micro kit (Qiagen). The quality of the isolated RNA was determined with a Fragment Analyzer (Agilent, Santa Clara, California, USA). The TruSeq Stranded mRNA kit (Illumina, Inc, California, USA) was used in the subsequent steps. Briefly, total RNA samples (60 ng) were polyA enriched and then reverse-transcribed into double-stranded cDNA. The cDNA samples were fragmented, end-repaired and adenylated before ligation of TruSeq adapters containing unique dual indices (UDI) for multiplexing.