GSM5393377: pOD4_PAM_lib_deplete_3; Escherichia coli; OTHER
Plasmids were isolated using ZymoPURE II Plasmid Midiprep Kit. Libraries were constructed by amplifying plasmid DNA region with primers containing Illumina adapters for 25 cycle using Q5 HS High-Fidelity 2X Master Mix. Following amplification, samples were cleaned up with AMPure XP beads. These dsDNAs were then indexed using Nextera barcodes 501, 502, and 707, 708.