GSM5090469: L3: PAH-PASMCs; Homo sapiens; RNA-Seq
Total RNA was isolated from human PASMCs using Trizol reagent (Invitrogen) according to the manufacturer's instructions. The quality and concentration of total RNA were then determined with a spectrophotometer (NanoDrop 2000, Thermo Scientific, USA). RNA integrity was confirmed by electrophoresis on a denaturing agarose gel. RNA sequencing analysis was carried out on total RNA extracted from PAH-PASMCs with or without siRNA-mediated silencing of EZH2. Qualitative and quantitative analysis of RNA was performed using NanoDrop2000 spectrophotometer (Thermo Scientific, USA). Library preparation and paired end RNAseq were performed by Cofactor genomics (St. Louis, MO) using Illumina HiSeq, on the PASMC samples at a read length of 150 bp and an average read depth of 50 million read pairs. Total RNAseq (150 bp paired end, 50million clusters)