GSM5579748: Iswi_AID_antiFLAG_2_CUT&RUN; Drosophila melanogaster; OTHER
Cells were permeabilized and incubated with anti-FLAG M2 antibody, followed by pAG-Mnase. pAG-Mnase digestion was activated by addition of CaCl2 to 2mM for 2 hours at 4C. Soluble DNA fragments were cloned using NEBNext  Ultra II DNA Library Prep Kit