GSM5582170: Colon CIA ZT12 rep 4; Mus musculus; RNA-Seq
A 5mm section of mid-colon was snap frozen and prepared for RNA Seq.  Tissue was transferred to a Lysing matrix D tube containing Trizol.  Tissue was homogenized using a Bead Mill homogenizer.  RNA was extracted using chloroform then precipitated using isopropanol.  After washing with 75% ethanol, the RNA pellet was re-suspended in RNase free water (50mg/ml). RNA libraries were prepared for sequencing using standard Illumina protocols