GSM5089954: FAGs_A2 ; Bactrocera oleae; RNA-Seq
The RNA isolation from the tissues was performed based on the TRIzol Reagent following the manufacturer's instructions with slight modifications. The isolated mRNA was digested with RNaseIII, hybridized and ligated to Ion Adaptors, reverse transcribed, barcoded and amplified, using the Ion Total RNA-Seq Kit v2 (Life Technologies, Carlsbad, CA, USA).