GSM6025898: human ventricular cardiac tissue strip [CTS-2_S38_L004]; Homo sapiens; RNA-Seq
All tissue constructs were washed with PBS without Ca2+ and Mg2+ and incubated in a solution containing 10 unit/mL of papain (Sigma, 76220) and 1 mM of EDTA (Roth, 8043.2) for 45 min at 37°C. The cells were then gently triturated for ~1 min until a single-cell suspension was obtained. Medium with 10% fetal calf serum (FCS, Merck-Milipore, S 0615) was added to inhibit the digestion process. The cells were then washed in PBS and counted. Single Cell 3' Reagent Kit v2 (Chromium Single Cell 3' Library & Gel Bead Kit v2, Chromium Single Cell A Chip Kit, 48 runs, 10x Genomics) and Chromium Controller (10x Genomics)