GSM6267500: P0 Emcn neg, 1; Mus musculus; RNA-Seq
Total RNAs were extracted using the Trizol reagent. Their integrity and quantity were examined using the RNA screentape on a 4200 TapeStation system (Agilent). Samples with RNA integrity number above 7 were used for library construction. RNA-seq libraries were generated with the KAPA RNA HyperPrep Kit (Roche, no. KK8581) according to the manufacturer's protocol