GSM5291672: dosR mutant Rep 1, DMSO; Mycobacteroides abscessus ATCC 19977; RNA-Seq
The cultures were treated with 2 volumes of GTC buffer and collected by centrifugation . Cells were lysed using a bead beater. Total RNA was prepared from the pellets using the Direct-zol™ RNA Miniprep kit. cDNA libraries were constructed using the Illumina TruSeq RNA library preparation kit (v2), omitting the polyA selection step. Multiplexing sequences are included in the title of the associated fastq.gz files. After library quality control, sample libraries were pooled and sequenced in two lanes of an Illumina HiSeq 2500 Rapid Run flow cell (v1) in 50bp, paired-end read format (SE50). After the sequencing run, reads were de-multiplexed and converted to FASTQ format using the Illumina bcl2fastq (v1.8.4) script.