GSM5195349: 6-012 (miRNA); Homo sapiens; miRNA-Seq
RNA was extracted from freshly frozen biopsy tissue with TRIzol (Ambion, Waltham, Massachusetts) and chloroform.  After centrifugation, the aqueous phase was purified with an RNeasy Mini Kit (Qiagen, Valencia, California). The quality of total RNA samples was assessed by using an Agilent bioanalyzer (Santa Clara, CA). RNA libraries were prepared according to the manufacturer's instructions for the TruSeq Stranded Total RNA Library preparation kit (Illumina, San Diego, CA). Unique indices, included in the standard TruSeq kits were incorporated at the adaptor ligation step for multiplex sample loading on the flow cells. The flow cells were sequenced as 101×2 paired-end reads on an Illumina HiSeq 4000