Illumina NovaSeq 6000 sequencing; 3' RNAseq of EGFR mutant lung cancer cell line overexpressing BRAF V600E treated with inhibitors
Cells were treated with osimertinib, trametinib, a combination of both or MDSO for 48h prior to harvest. RNA was extracted using Qiagen RNeasy kits. Sequencing libraries were prepared using the QuantSeq 3' mRNA-Seq library kit (Lexogen, Austria)