GSM4725804: 12-Control; Mus musculus; RNA-Seq
Mouse organs were stored in TRIzol (Thermo Fisher) at –80°C until analysis. Samples were thawed on ice and allowed to equilibrate to room temperature before RNA was isolated using the RNeasy Mini kit (Qiagen) following the manufacturer's protocol. After the quality of RNA was assessed using a 2100 Bioanalyzer (Agilent), samples were prepared using a QuantSeq 3′ mRNA-Seq library prep kit FWD for Illumina (Lexogen) following the manufacturer's protocol and analyzed on a NextSeq 500 sequencing system(Illumina).