GSM5394138: B_0_p3; Oryzias latipes; RNA-Seq
Primordial germ cells (PGCs) were isolated according to the method developed by Wang and Bhandari (2020). Genomic DNA and total RNA were extracted using ZR-Duet™ DNA/RNA MiniPrep kit (Zymo Research, Irvine, U.S.A.). RNA sequencing libraries were prepared with NEBNext® UltraTM II RNA Library Prep Kit for Illumina accordingly to the manufacturer's instructional manual. For each sample, 500ng total RNA was used. RNAseq libraries were sequenced on the Illumina platform using the 150 bp pair-end sequencing strategy.