GSM5620633: Pre-malignant_KT_RNA_4; Mus musculus; RNA-Seq
Pre-malignant lung epithelial cells were sorted by FACS, and part of them were then lysed dicrectly to release total RNA and genomic DNA. The remaining cells were collected and used to extract RNA and DNA by AllPrep DNA/RNA Micro Kit (Qiagen, 80284). Parental KT LUAD cells and KT LUAD cells complemented with Tet2CD or Tet2HD were harvested and then used to extract RNA and DNA by AllPrep DNA/RNA Mini Kit (Qiagen, 0204) RNA libraries of pre-malignant cells were constructed by Smart-seq2 protocol.PBAT libraries of pre-malignant cells were constructed by PBAT protocol (Dai et al., 2016, Nature). RNA libraries of LUAD cell lines were constructed by kit (Vazyme, NR611). TAPS libraries of LUAD cell lines were constructed as described in the paper of Liu et al., 2019, Nature Biotechnology. RNA-seq, WGBS (whole-genome bisulfite sequencing), TAPS (TET-assisted pyridine borane sequencing)