GSM5291700: 19720_APPFCre_M; Mus musculus; RNA-Seq
Total RNA was extracted from the frontal cortex using AurumTM Total RNA Fatty and Fibrous Tissue Kit (732-6830; Bio-Rad) following the manufacturer's instructions. RNA quantity was measured using Quant-iT RiboGreen Assay Kit (Thermofisher), and the quality was determined by the Agilent Bioanalyzer system. mRNA libraries were prepared following Illumina TruSeq Stranded mRNA library prep workflow