GSM5225892: LOCE_2_Ctrl; Homo sapiens; RNA-Seq
Total RNA was extracted using the innuPREP RNA mini kit (Analytik Jena), according to the manufacturer's instructions. RNA-seq libraries were prepared according to the Illumina Truseq stranded mRNA sample preparation guide. Final libraries were pooled and sequenced on a HiSeq4000 (LOCE#1 and #2 in Ham's F10 and Ham's F10 with tyrosine; Illumina) and NextSeq500 (LOCE#1 and #2 in Ham's F10 and RPMI1640; Illumina).