GSM5003947: 1-10; Homo sapiens; RNA-Seq
PBMC were collected using Ficoll-Paque™ PREMIUM Media and SepMate™-50, RNeasy Mini Kit was used to extract the total RNA. rRNA was removed by Epicentre Ribo-zero rRNA Removal Kit. The DNA was cleaned by DNase and then captured and purified by magnet beads. First-strand cDNA was synthesized and double-strand cDNA was obtained thereafter with VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina.