GSM5088692: iPS Cell derived RPE; Homo sapiens; RNA-Seq
Cells were digested to single cell with 0.25% trypsin/EDTA, screened, and resuspended in low FBS Phosphate Buffered Saline solution, counted 16 times for cell concentration, viability, and doublet rate, and then diluted to approprate loading concentrations for the 10X Genomics Chromium Controller. scRNA-seq libraries were prepared with 10x Genomics Chromium system using 10x Genomics 5' (v1.0 Chemistry) VDJ kit library construction with standard protocol for 5' Gene Expressoin libraries that skips Steps 4&5 and uses the i7 multipltex library Index kit following standard protocol. Libraries were diluted and submitted to Macrogen for sequencing under the library strategy.