GSM5004187: gastric cancer peritoneum metastasis P1; Homo sapiens; RNA-Seq
Tissues were washed twice by PBS supplied with 10% BSA (Sigma, USA), minced into small pieces with the size of 2–4 mm, and then dissociated by a Human Tumor Dissociation kit (Miltenyi Biotech, Germany). The only exception was the liver biopsy sample which was dissociated with the Mouse Tumor Dissociation kit (Miltenyi Biotech, Germany) based on the cell viability results of preliminary experiment. The cell suspension was further filtered through 70 μm SmarterStrainers (Miltenyi Biotech, Germany) to remove cell aggregates and re-suspended in PBS containing 10% BSA. Red blood cells were removed by a Red Blood Cell Lysis Solution (10×) (Miltenyi Biotech, Germany). Dead cells were eliminated by a Dead Cell Removal Kit (Miltenyi Biotech, Germany) to increase the efficiency of sorting robust, and the live cells were washed twice, re-suspended in PBS containing with 10% BSA, and then used for single-cell experiments. The cell suspension was further filtered through 70 μm SmarterStrainers (Miltenyi Biotech, Germany) to remove cell aggregates and re-suspended in PBS containing 10% BSA. Red blood cells were removed by a Red Blood Cell Lysis Solution (10×) (Miltenyi Biotech, Germany). Dead cells were eliminated by a Dead Cell Removal Kit (Miltenyi Biotech, Germany) to increase the efficiency of sorting robust, and the live cells were washed twice, re-suspended in PBS containing with 10% BSA, and then used for single-cell experiments.