GSM6267394: colon, KO-3; Mus musculus; RNA-Seq
Mice were euthanized and their colons dissected. Full-thickness distal colonic tissue was homogenized and lysed. RNA was prepared using OMEGA Bio-tek EZNA on-column isolation kit. Libraries were constructed using the Illumina Stranded mRNA library preparation kit to generate single-end reads 1x75bp on NextSeq High Output Runs.