GSM4998214: B1a Cic KO rep1 (IgKL); Mus musculus; RNA-Seq
Peritoneal cavity B-1a cells (IgM+, CD19+, CD5+, CD43+) were isolated by FACS-sorting using a MoFlo-XDP (Beckman Coulter). Total RNA was extracted using TRIzol Reagent (GeneAll, Seoul, Republic of Korea) according to the manufacturer's protocol. Long Read iR-Profile Reagent System (iRepertoire) was used to generate NGS libraries covering BCR chains including Igh, Igk, and Igl. Briefly, nested inside and outside primers selectively amplified all V- and C- regions and incorporated communal adaptors. Following clean up, only target amplicons, which contain 5' and 3' communal adaptors, were exponentially amplified.