GSM5194529: smRNA_P1T; Homo sapiens; miRNA-Seq
Total RNA was extracted using mirVana miRNA Isolation Kit (Ambion, Austin, TX) according to the manufacturer's protocol. Quantitation of total RNA was carried out using the Nanodrop 2000 (Thermo Fisher, 5Waltham, MA). RNA integrity was assessed by Agilent 2100 Bioanalyzer (Agilent Technology, Santa Clara, CA). Total RNA of each sample was used for the small RNA library construction using TruSeq Small RNA Sample Prep Kits (Cat. No. RS-200-0012, Illumina, San Diego, CA) following the manufacturer's recommendations.