GSM5267054: 05-14_WT_281_S4; Mus musculus; RNA-Seq
12-20 cochlear sensory epithelia were dissected, then collected into a tube. After being trypsinized with TrypLE and mechinically dissociated, the cells were pelletized with a centrifuge. The pellet was suspended in PBS, then hair cells were sorted with a hair cell marker, Atoh1-GFP. Total RNA was extracted from the sorted hair cells using the RNeasy Plus Micro Kit. Full-length cDNA was prepared using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Bio USA, Inc., 640170) with 1.6-2.4 pg of total RNA and sequencing libraries were prepared using the Nextera XT DNA Library Preparation Kit (Illumina, FC-131-1096).