GSM5548311: Vehicle_7_l2; Mesocricetus auratus; RNA-Seq
RNA extraction was performed following instructions from the manufacturer of TRIzol reagent (Invitrogen). Briefly, 1/5 volume of chloroform was added to the lung supernatants in TRIzol, phases were separated by centrifugation and RNA was precipitated by overnight incubation with isopropanol at -20C. The RNA pellet was washed with ethanol 70% and resuspended in RNase-free water. RNA was quantified by nanodrop and resuspended to a final concentration of 100 ng/ul in water. 1 ug of RNA was used as starting material for library preparation. The kit employed was TruSeq RNA Library Prep Kit v2 (Illumina) over polyadenylated RNA and the manufacturer's instructions were followed. The sequencing was performed on an Illumina NextSeq 500 instrument.