GSM6165048: Eluate, 10 nM; Severe acute respiratory syndrome coronavirus 2; RNA-Seq
mRNA display was performed on a library of variants. Peptides were selected based on their ability to bind to a His6-tagged HR1 peptide, which was purified with Ni-NTA beads. Peptide-mRNA fusions were reverse-transcribed using Superscript III (ThermoFisher) and oligo-dT(20-mer) as an RT primer. After precipitation, cDNA was PCR-amplified, agarose-gel purified, and sequenced.