GSM5004126: JB116_RNA; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol™ reagent and was further purified using RNeasy MiniElute Cleanup kit, including a DNase digest. cDNA libraries were prepared for each sample using the Illumina TruSeq RNA Sample Preparation Kit following the manufacture's recommended procedures. Libraries were sequenced using 100 base pair (bp) paired-end reads on the Illumina HiSeq 2500 platform.  Then DNA-sequencing was conducted using the Illumina HiSeq 2500  platform. The sequencing libraries were generated on the streptavidin beads using the Nextera DNA Library Prep Kit.