GSM5513165: Monocyte_64-V7; Homo sapiens; RNA-Seq
RNA was extracted from sorted cells from PBMC of HVTN 505 vaccinees using RNAzolRT (MRC Inc.) as per recommendations from the manufacturer. Polyadenylated transcripts were purified on oligo-dT magnetic beads, fragmented, reverse transcribed using random hexamers and incorporated into barcoded cDNA libraries based on the Illumina TruSeq platform. Next, libraries were validated by electrophoresis, quantified, pooled and clustered on Illumina TruSeq v2 flow cells. Clustered flow cells were sequenced on an Illumina HiSeq (2000/4000) using 2 x 75 base paired-end runs.