GSM5195617: Primary PDAC tissue 3; Homo sapiens; RNA-Seq
Snap-frozen tumor tissues were carried on dry ice. Lysis buffer using AllPrep DNA/RNA mini kit (QIAGEN) was added to the samples according to manufacturer's protocol; samples were processed as quickly as possible with homogenization using Ultra-Turrax T25 homogenizer (Janke & Kunkel).  Lysed and homogenized tissues were processed for DNA/RNA extraction using AllPrep DNA/RNA mini kit (QIAGEN) according to manufacturer's instructions.    Organoids were extracted from matrigel domes using Cell Recovery Solution (Corning, #354253). Organoids were centrifuged and washed with PBS twice to discard remaining matrigel. DNA and RNA were extracted simultaneously using AllPrep DNA/RNA mini kit (QIAGEN) according to manufacturer's instructions.