GSM5580185: RNA-Seq CD4_withoutMHE_PC149; Homo sapiens; RNA-Seq
CD4 T cells were isolated using EasySep™ Human CD4+ T Cell Isolation Kit (STEMCELL Technologies Inc.) and RNA isolation was made with miRNeasy Micro Kit (QIAGEN). HiSeq Sequencing v4 Chemistry was used for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols