GSM5958680: select_wt_replicate1_tile2; synthetic construct; OTHER
For each condition (select/nonselect, library/wt, replicate1/replicate2), 9 OD units of cells were used for plasmid DNA extraction using the ChargeSwitch Plasmid Yeast Mini kit (Invitrogen) according to the manufacturer's instructions. The three regional libraries spanning the GCK sequence (aa 2-171, 172-337, 338-466) were constructed by pooled mutagenesis using NNK-degenerate oligos.