GSM4818824: Cell #172; Homo sapiens; RNA-Seq
Following patch-clamp recording, each whole neuron (soma and neurites ≥150 μm away from the cell body) was collected and lysed in sample collection buffer as previously detailed in our Patch-seq protocol (van den Hurk et al., 2018). The SMARTer® Ultra® Low Input RNA for Illumina Sequencing kit (Clontech, Cat. No. 634828) was used for reverse transcription of single-cell RNA and subsequent amplification/purification of full-length cDNA. Construction of Illumina sequencing libraries was performed using Nextera XT DNA Library Preparation Kit (Cat. No. FC-131-1096) and Indices (Cat. No. FC-131-1001) using 0.25 ng of full-length cDNA.