GSM5226355: GIST-T1 H3K4me3; Homo sapiens; ChIP-Seq
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Library construction was performed according to the manufacturer's instructions. Briefly, 100 ng of input DNA or the immunoprecipitate was ligated to adapters and PCR amplified using a SOLiD Fragment Library Construction kit (Applied Biosystems). Template bead preparation was carried out using a SOLiD ePCR kit V2 (Applied Biosystems) and a SOLiD Bead Enrichment kit (Applied Biosystems).