GSM5516045: E12.5 Stromal cells LI replicate 01; Mus musculus; RNA-Seq
Two independent experiments with three-five embryos/experiment. Small and large intestine (including cecum) were dissected out prior to single cell suspensions and sorting (Live, CD45-, Ter119-, CD31-, EpCAM-, Itgb1+, YFP+) of mesenchymal stromal cells for scRNA-seq. Single cell RNA-seq libraries were prepared according to the manufacturer's instructions using Chromium Single Cell 3' Library & Gel Bead Kit v3 (10x Genomics, PN-1000092) or 5' kit Chromium Single Cell 5′ Library & Gel Bead Kit (10x Genomics, PN-1000006), and Chromium Chip B Single Cell Kit (PN-1000074) with the Chromium Controller & Next GEM Accessory Kit (10x Genomics, PN-120223).