GSM5582421: RIP_4A1_siControl_pulldown_replicate2; Homo sapiens; RIP-Seq
Cells were directly lysed in lysis buffer on culture dishes. For ribosome profiling, RNase I treatment, ribosome pelleting, size selection, preadenylated linker ligation, rRNA depeletion, reverse transcription, cDNA circularization, and PCR amplification. For RNA-Seq, RNA extraction and TruSeq Stranded mRNA Library Prep Kit (Illumina). For RIP-Seq, pulldown by streptavidin beads, elution by biotin, RNA extraction, and TruSeq Stranded mRNA Library Prep Kit (Illumina).