GSM6327543: 11M-MP-P1-D3-F9_S1701; Mus musculus; RNA-Seq
Single squamous cell cancer cells were FACS purified, and collected into 96-well plates containing lysis buffer and snap frozen in liquid nitrogen. Plates were stored at -80oC Single cells were lyzed by incubating at 72oC for 3 min. Reverse transcription reaction and whole transcriptome amplification were performed according to Smart-seq2 protocol. The amplified cDNA libraries were purified by Ampure XP beads (Beckman Coulter), and the tagmentation reaction was performed by Illumina Nextera XT DNA Library Prep Kit. The libraries were pooled and sequenced on an Illumina NovaSeq SP with 50 cycles paired-end mode (2 lanes).