Transcriptome of Vibrio parahaemolyticus strain AHPND
Bacterial cells were collected from culture of VPAHPND with the absence (control) and presence of 2.5% V. alginolyticus extract. The bacterial culture was centrifuged at 8,000 xg for 10 min, and added 1 ml RNAprotect Bacteria Reagent (Qiagen, Germany). Total RNA from the two samples was extracted using the TRIzolTM Reagent (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA) following its manual. RNA sequencing was performed using NovaSeq 6000 system (Macrogen, Korea). Sequence data was mapped to reference genome with Bowtie software. Read count per gene was extracted from known gene annotations with HTSeq program. The evaluating gene expression profiles were performed using FPKM method (fragments per kilobase of transcript per million mapped reads) as                 a normalization value. Statistical analysis was performed using fold change and exactTest. The significant results were considered on conditions of lfcl 2& exactTest raw p-value < 0.05.