GSM5005273: OKseq_DMD_locus_rep3; Gallus gallus; OTHER
Asynchronously growing cells were labelled with 20 μM 5-ethynyl-2'-deoxyuridine (EdU, Jenabioscience) for 2 minutes. After DNA purification and heat-denaturation, Okazaki fragments were size-purified on sucrose gradients, labelled with biotin at EdU sites using click-chemistry, captured on magnetic beads coated with streptavidin and amplified by PCR. Libraries were prepared using standard Illumina protocols.