GSM6327178: MTO54 parental Folfiri2; Mus musculus; RNA-Seq
Cell media was aspirated and 400 μl of RNA Lysis buffer supplemented with β-mercaptoethanol were added per well. RNA was purified using PureLink RNA mini kit (12183025, Thermo Fisher) following manufacturer´s instructions adding a Dnase step using PureLink DNase set (12185010, Thermo Fisher). RNA libraries were prepared for sequencing using the kits NEBNext® Poly(A) mRNA Magnetic Isolation Module (Cat# E7490) and NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (Cat#E7770), following manufacturer´s instructions and 8 cycles of library amplification