GSM5350749: ref6c-H3K27me3-72h_rep2; Arabidopsis thaliana; ChIP-Seq
Materials were ground with liquid nitrogen into fine powder and fixed with 1% formaldehyde. To profile H3K27me3, nuclei were extracted and mononucleosomes were generated with micrococcal nuclease (MNase)(Sigma, N5386) digestion. Immunoprecipitation was conducted with anti-H3K27me3 antibody (Merck, 07-449). After antibody incubation, Protein A Dynabeads (Life technologies, 10002D) were added to collect the immunocomplexes, followed by elution, reverse cross-linking and DNA purification. Library was prepared with VAHTS universal DNA library prep kit for illumina (Vazyme, ND607) according to the manufacture's instruction