GSM5841539: Fetal MID gestation exposure replicate 2; Ovis aries; miRNA-Seq
Total RNA was extracted with Trizol according to manufacturer's specifications. Quantification and qualification were checked by 1% agarose gel, NanoPhotometer spectrophotometer, and Agilent Bioanalyzer 2100 system. The NEBNext Multiplex Small RNA Library Prep Set for Illumina following manufacturer's recommendation and index codes were used to prepare the sequencing library.