sCRilecs-seq of S. pneumoniae: FACS sort based on FSC
A genome-wide CRISPRi library where sgRNA sequences were integrated in the S. pneumoniae genome was created in S. pneumoniae D39V and D39V with deleted SPV1536-1537 (=Dmev). After selection by FACS sorting based on FSC, sgRNA sequences were amplified and sequenced using Illumina MiniSeq.