RNA-seq of Caretta caretta: hatchling gonad
Targeted tissues, including brain and gonad, were immediately placed into RNAlater reagent (Qiagen, Inc.) for high quality RNA stabilization after dissection. Tissues were homogenized and purified, and cDNA was extracted using a Qiagen TissueRuptor, RNeasy, and Omniscript Reverse Transcription (RT) kit. RNA samples were quantified via an Agilent Bioanalyzer prior to Illumina TruSeq library preparation