Oxford Nanopore MinION basecalled long reads of Holacanthus passer: adult female gill
Four individual ONT libraries were prepared using the SQK-LSK109 library prep protocol according to manufacturers protocol (Oxford Nanopore Technologies, Oxford, UK). Each library was sequenced on a R9.4 flow cell using the MinION DNA sequencer. Raw data was basecalled separately using Guppy 3.3 basecaller on a GPU-based high-performance computer cluster.