DNA-Seq of Escherichia coli JW5503 using Oxford Nanopore
The genomic DNA was extracted from the overnight culture in 2xYT medium using the UltraClean Microbial DNA Isolation Kit according to the manufacturers instructions (Qiagen). The library was prepared using the Oxford Nanopore Ligation Sequencing Kit (SQK-LSK109) and sequenced using a FLO-MIN106 R9.4.1 flow cell with the MinION Mk1B device. The raw reads were base-called using DeepNano-blitz at high accuracy mode (--network-type 96).