GSM5350821: ECRT-SMARCA4, Patient_2; Homo sapiens; RNA-Seq
Total RNAs were obtained from tumor frozen samples using Qiagen RNAeasy kit, according to the manufacturer's procedures (Qiage, Venlo, Netherlands) Barcoded Illumina compatible libraries were generated from 750 ng of total RNA for each sample using TruSeq Stranded mRNA Library Preparation Kit (Illumina, San Diego, California, U.S.) Library werer sequenced using the Illumina HiSeq 2500 platform in the 100 bp paired-end mode