Staphylococcus coagulans strains 1031336 whole genome sequencing
Whole-genome sequencing libraries will be prepared using NEBNext Ultra DNA Library preparation kit. In brief, the fragmented DNA is subjected to a sequence of enzymatic steps for repairing the ends and tailing with dA-tail followed by ligation of adapter sequences. These adapter ligated fragments are then cleaned up using SPRI beads. Next, the clean fragments are indexed using limited cycle PCR to generate final libraries for paired-end sequencing. The resulting libraries are quantified before loading on the cBot for cluster generation and sequencing on Illumina HiSeq system to generate 2X150bp sequence reads.