Ion Torrent S5 XL sequencing; Changes in gene expression in Neural crest boundary cap cells after space flight
Changes in gene expression in Neural crest boundary cap cells after space flight
BC neurospheres derived from space cells and earth controls were grown in stem cell medium for one to four weeks after space voyage. RNA was extracted using an RNAEasy Plus Micro kit (Qiagen), according to the manufacturer's instructions. Concentrations were measured using an ND-1000 spectrophotometer (NanoDrop Technologies) and subsequently, RNA integrity was controlled by using an Agilent 2100 Bioanalyzer. Ten ng of RNA was used for each sample.  The libraries were prepared for sequencing on the Ion Chef System and sequenced on the Ion S5 XL on 540 chips using IonCode adapters. Sequencing libraries for 16 individual samples pre-pared from 180–280 ng total RNA using the Ion AmpliSeq™ Transcriptome Mouse Gene Expression Kit (Cat. Nos. A36553, A36554, and A36555, Thermo Fischer Scientific) according to the manufacturer's protocol.