Illumina NovaSeq 6000 sequencing; RNA-seq of human glioblastoma cell lines with overexpression of SOX2, SFRP2
RNA-seq of human glioblastoma cell lines with overexpression of SOX2, SFRP2
Cells are seed in the plate until 80% confluence. Then cells were washed by PBS twice, and lysed with the lysis buffer. Cells were maintained in Minimum Essential Medium (MEM) with10% FBS and penicillin-streptomycin at 37°C with 5% CO2 and 5% O2 and over 95% humidify.  SOX2 and SFRP2 transduction in glioblastoma cells were performed using lentiviral transfection systme. Cells were selected by using puromycin. Total RNA was purified using the PureLink® kit following the manufacturer's instructions (Thermo Fisher Scientific) Illumina TruSeq strand-specific RNA libraries (poly-A selection)