Human RPE Cell free miRNA: Extracellular vesicles
miRNA libraries were prepared from the isolated EV RNA and a water control using the TruSeq Small RNA Library Preparation Kit (RS-200-0012, Illumina, San Diego, CA, USA) using the manufacturer instructions with the modification of performing the adaptor ligation in the presence of 15% polyethylene glycol in order to reduce bias by driving the ligation reactions towards completion as determined by the Extracellular RNA Communication Consortium