GSM4820830: Wistar WU 5; Rattus norvegicus; miRNA-Seq
Retinas were removed, flash frozen on dry ice, and total RNA was extracted using NucleoSpin miRNA kit (Macherey–Nagel, Düren, Germany) following manufacturer's instructions. Small RNA library construction from pooled retinal samples (N=3, in each age-group) were carried out according to the Ion Total RNA-Seq Kit v2 protocol (Revision E) with slight modification. Enzymatic reaction was performed in half reaction volume, while cleaning procedures were executed with the accurate final volume according to the protocol.