RNA-Seq of Corinectria sp. strain N1 (NfP5.7): active mycelium
RNA was isolated from the mycelium of a 21-day-old fungus culture of the NfP5.7 strain grown on 2% malt extract agar using the Plant/Fungi Total RNA Purification Kit. The NEBNext Poly(A) mRNA Magnetic Isolation Module was used to separate intact poly(A)+ RNA from the total extracted RNA. The TruSeq Stranded Total RNA with Ribo-Zero Plant kit was used to remove rRNA, convert mRNA to cDNA, and generate the paired-end sequencing libraries that were sequenced on an Illumina MiSeq with 2x75 cycles using the MiSeq Reagent Kit v3 for 150 cycles.