16sRNA-seq of TiO2-treated zebrafish gill 1
20-30 ng DNA from zebrafish gill sample was used to generate amplicons.V3 and V4 hypervariable regions of prokaryotic 16S rDNA were selected for generating amplicons and following taxonomy analysis. GENEWIZ designed a panel of proprietary primers aimed at relatively conserved regions bordering the V3 and V4 hypervariable regions of bacteria and Archaea16S rDNA. A linker with Index is added to the end of the PCR product of 16S rDNA by PCR for NGS sequencing, the library is purified with magnetic beads, and the concentration is detected by a microplate reader and the fragment size is detected by agarose gel electrophoresis.