Shotgun sequencing of purified RhCMV 681 BAC with EBOV transgene
100ng of BAC DNA was sheared in a Covaris S220 sonicator to aproximately 450bp and a sequencing library was prepared by carrying out seven cycles of PCR with indexed primers (New England Biolabs) using a Kapa LTP library preparation kit. Libraries were sequenced using a NextSeq 550  (Illumina).