ITS1 nrDNA-Seq of soil mycobiome: Onion soil
The DNA was extracted from 250 mg soil sample using Qiagen Dneasy PowrSoil DNA extraction kit. The ITS1 region was amplified in duplicates using the barcoded forward primer ITS1FKYO2 and the reverse primer ITS2KYO2 using BioRad T100 Thermal Cycler. The two products were mixed together then purified and quantified. Emulsion PCR was conducted using Ion PGM Hi-Q View OT2 kit 400 on the Ion OneTouchTM 2 system. Sequencing was then performed using Ion Personal Genome Machine with Ion PGM sequencing 400 Kit and Ion 316TM chip v2 BC (Life Technology, Inc.).