GSM5417039: ZSF-1OB Gr1 Vehicle ctrl 1012 Kidney; Rattus norvegicus; RNA-Seq
Kidney tissues were extracted from experimental animals and immediately snap-frozen in liquid nitrogen and stored at −80°C until used. Stat-60 solution was used to homogenize the samples. The homogenate was then transferred to an RNase-free centrifuge tube, after which RNase-free water were added. Samples were incubated, centrifuged, and the supernatant collected. The aqueous phase into a total RNA extraction procedure that is bead based MagMax MirVana (A27828, Thermo Fisher Scientific, Waltham, MA)  that was automated on a KingFisher instrument(Thermo Fisher Scientific, Waltham, MA). The RNA quantity and quality was assessed using a Fragment Analyzer. Equal molar concentration of about 0.75nM as the running concentration for all samples. RNA libraries were prepared for sequencing using standard Illumina protocols