GSM5461527: RNA-Seq WSB-F; Mus musculus; RNA-Seq
Whole-islet RNA was isolated and evaluated for quality. RNA samples were submitted to the Jackson Laboratory high-throughput sequencing core facility. Sample, collection, RNA extraction and sequencing was carried out on in four waves of animals. RNAs were sheared using the E220 Focused-ultrasonicator (Covaris).  The Jackson Laboratory core generated whole genome islet mRNA libraries using the KAPA Hyper Prep Kit for Illumina Sequencing (KAPA Biosystems), targeting an insert size of 300 bp using magnetic bead-based size selection. Libraries from waves 1, 2, and 3 were constructed at the Jackson Laboratory and randomized into pools of samples with TruSeq RNA indices (6 bp). Libraries from wave 4 were constructed at the New York Genome Center and were pooled using 8-bp dual index TruSeq RNA indices. Each pooled RNA sample was sequenced across four randomly assigned lanes on a HiSeq2500 (Illumina) at the New York Genome Center.