metagenomics of the gut contents of Zhongdian Yak cows
DNA was extracted by using PowerSoil DNA Isolation kit (MO BIO Laboratories, Inc., Carlsbad, CA). The quality and quantity of DNA were measured using Qubit V.2.0 fluorometer quantitation (Thermo Fisher Scientific, Inc., Waltham, MA, USA). The genomic DNA was randomly sheared into fragments and consructed library using TrueLib DNA Library Rapid Prep Kit for Illumina (ExCell Bio). Metagenome libraries sequencing was performed on an Illumina Novaseq PE150 (150 bp paired-end sequencing, 500 pb inserts) at Biomarker Technologies Co. Ltd. (Beijing, China).