GSM5514979: Has2+/- KO mice 24 after chronic OVA stimulation 1; Mus musculus; RNA-Seq
Lung were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. RNA sequencing (RNA-seq) libraries were prepared using a NEBNext rRNA Depletion Kit (New England Biolabs, Ipswich, MA) and an ENBNext Ultra Directional RNA Library Prep Kit (New England Biolabs) according to the manufacturer's instructions using 500 ng of total RNA samples. RNA libraries were prepared for sequencing using standard Illumina protocols