GSM5583270: W45_6wk; Rattus norvegicus; Hi-C
Centrifugation was used to remove fat and collect the cell pellets.  Individual cell pellets were washed and resuspended in DMEM/F12 media.  Each cell suspension was filtered using 40μm nylon to enrich the mammary ductal fragments and remove stromal cells.  The filter was inverted and rinsed to collect the fragments, and the resulting cell pellet containing mammary epithelial cells was diluted in PBS and treated for 10 minutes with 1.5% formaldehyde for DNA/chromatin fixation.  After a series of washes, the final cell pellets were collected using centrifugation and stored at -80oC. A total of 6 samples were sent to Arima Genomic, Inc. (n=3 from each age group) for Arima HiC Service, consisting of complete sample processing for Arima-HiC and library preparation, Illumina Next-Generation sequencing and data upload.