Genome skimming: Astragalus obscurus - HN 2002583
Fragmentation with Bioruptor Pico sonicator using 7 cycles of 15 s On/90 s Off; NEBNext Ultra II DNA Library Prep Kit with no size selection and half volumes, 6 PCR cycles using NEBNext Dual Index Primers i709 and i501; Index Read 1: CGGCTATG, Index Read 2: AGGCTATA