GSM5229016: DS40_muAs-2; Mus musculus; RNA-Seq
Spheres were washed with PBS and frozen with Trizol. RNA was isolated using Trizol and chloroform and purified using the RNeasy Mini Kit (QIAGEN). Illumina sequencing libraries were constructed from 300-500ng of total RNA using the TruSeq Stranded mRNA Library preparation kit (Illumina).