GSM5461383: AGM progenitors; Mus musculus; RNA-Seq
The AGM and fetal liver were isolated from E11.5 embyros as delineated in the Methods section. Each tissue was dissociated in 0.125% collagenase type I at 37°C for 30 minutes, after which cells were further dissociated by gentle pipetting and then passed through a 40 µm strainer. Single cells were washed with PBS and then incubated with anti-mouse CD16/32 at 4°C for 10 minutes for Fc blocking prior to antibody staining. Single-cell encapsulation was performed using 10X Chromium single cell 3' technology. Whole-transcriptome libraries were prepared using the 10X v3 protocol and sequenced on Illumina NextSeq500 paired-end 150 cycles (Read 1: 28 cycles; Index Read 1 (i7): 8 cycles; Read 2: 91 cycles).