GSM5777361: an1_18h; [Haemophilus] ducreyi; RNA-Seq
5 mL of culture was pelleted, media was removed and the pellet was resuspended in 1 mL of Trizol. Samples were stored at -80 until all samples were collected. RNA was extracted using the Zymo Directzol kit with both on-column DNase digestion as well as a subsequent DNase digestion of the eluate with TurboDNase. Ribosomal RNA was removed with Qiagen FastSelect for bacteria. Libraries were prepared with a Roche KAPA kit.