GSM5582360: N-21.6_RNA; Homo sapiens; RNA-Seq
Magnetic-activated cell sorting (MACS) with anti-CD45 MACS cell separation microbeads and reagents (Miltenyi Biotec). RNA was extracted with the Direct-zol RNA purification kit (Zymo R2061). Sequencing libraries were prepared using the TruSeq Stranded mRNA library prep kit (Illumina 20020594) from 500ng input RNA. Samples were barcoded and run on a Hi-seq 4000 in a 100bp/100bp paired-end run, using the Hi-seq 3000/4000 SBS kit (Illumina).