NextSeq 550 sequencing; Bulk RNA-seq of human induced pluripotent stem cells (hIPSC) and neural progenitor cells (NPC) differentiated usind Dual SMAD inhibition using the prime-seq method
Bulk RNA-seq of human induced pluripotent stem cells (hIPSC) and neural progenitor cells (NPC) differentiated usind Dual SMAD inhibition using the prime-seq method
Cells were cultured and differentiated into NPCs as described previously (Geuder et. al. 2021). One colony per sample was harvested and lysed in 100 µl RLT plus lysis buffer containing 1% beta-mercapto Ethanol and frozen at -80 °C. RNA extraction using carboxyl coated beads as described in the prime-seq protocol The prime-seq protocol was performed as described here: https://www.protocols.io/view/prime-seq-s9veh66