RNA-Seq of Sarcocyst: liver
DNA was extracted from liver from the alpacas using a commercial DNA extraction kit (DP304; Tiangen Biotec Company, China). The PCR products were sent to Shanghai Sangon Company (China) for bi-directional sequencing. The obtained sequences were analyzed by BLAST (GenBank database). The phylogenetic tree of the Sarcocystis spp. was constructed using the neighbor-joining method (Kimura two-parameter model).