GSM5005284: iNOS_Ly6c_M2; Mus musculus; RNA-Seq
24 hours post infection spleens were removed squashed against 70 um strainer followed by red blood lysis and stained with antibodies. 1000 cells of CD9 Mf (Cd3e-Cd19-Ly6g-NK1.1-CD11B+F4/80+Ly6C-CD9+) and iNOS Mf (Cd3e-Cd19-Ly6g-NK1.1-CD11B+F4/80+Ly6C+CD9-) were sorted to lysis buffer (0.1% Triton-100, 0.5U/Ml RNasin+ (promega). RNA-seq libraries were prepared according to Cel-seq2 libraries protocol (Hashimshony, T. et al Genome Biology 2016)