GSM6165473: BiSulfite-seq pol2a-1_meiocyte_2; Arabidopsis thaliana; Bisulfite-Seq
For meiocytes, over 1500 meiocytes were used to extract ~30-100 ng DNA for each biological replicate using the ChargeSwitch gDNA Micro Tissue Kit (ThermoFisher, CS11203). For seedlings, 4-5 plants were ground in liquid nitrogen and the genomic DNA was extracted by the CTAB method. A total amount of 100 ng genomic DNA spiked with 0.5 ng lambda DNA were fragmented by sonication to 200-300 bp with Covaris S220. These DNA fragments were treated with bisulfite using EZ DNA Methylation-GoldTM Kit (Zymo Research), and the library was constructed by Novogene Corporation (Beijing, China).