GSM5202085: row_RNAi2_rep3; Drosophila melanogaster; RNA-Seq
The flies were raised for three more days to recover from CO2 exposure and collected using dry ice. RNA was prepared using TRI Reagent (SIGMA) according to the manufacturer's protocol 3' RNA-seq library was performed based on Guttman's lab "RNAtag Seq protocol" (Shishkin, A. A. et al. Simultaneous generation of many RNA-seq libraries in a single reaction. Nat. Methods 12, 323–325 (2015).)