GSM5417527: S07_216; Homo sapiens; RNA-Seq
[Macrophage isolation human]  Mechanical tissue dissociation followed by percoll gradient centrifugation. FACS sorting for CD45posCD11Bpos cells. [Macrophage isolation mouse]  Enzymatic isolation at 4C using protease from Bacillus lichenformis and L-cysteine. FACS sorted on tdTomato Red cells. Qiagen Rneasy lipid mini kit 3' Quantseq FWD kit from Lexogen for bulk RNAseq. 3' single cell RNAseq v2 from 10x Genomics for single cell.