GSM5643582: chemo disease endpoint scRNA-seq pool 1; Mus musculus; RNA-Seq
Viable cells were sorted on the BD FACSAria III directly into Eppendorf tubes. The cells were then washed twice with PBS + 0.04% BSA. Cells were then spun down and resuspended in PBS + 0.04% BSA + 0.4 U/ uL RNase Inhibitor (Roche) to a final cell concentrationof >1500 cells per uL. The final cell suspension was put through a cell strainer then onto a 10X Chromium Chip Single cells were captured in droplet emulsions using the 10X Chromium Single-Cell Instrument and reverse transcription, cDNA amplification and library preparation were performed based on the manufacturer's protocol using the Chromium Single Cell 3′ Library & Gel Bead Kit v3 (10X Genomics)