Illumina HiSeq 4000 paired end sequencing; ATAC-seq in the U2OS-AR cell line comparing cells treated for 4 hours with 5 nM R1881 to vehicle (DMSO) control-treated cells
ATAC-seq in the U2OS-AR cell line comparing cells treated for 4 hours with 5 nM R1881 to vehicle (DMSO) control-treated cells
ATAC-seq was performed with 100,000 cells per treatment, cells were grown in DMEM containing 5% fetal bovine serum. U2OS-AR cells where grown to approximately 90% confluence according to standard protocols in DMEM medium with 5% FBS. Cells were treated for 4 hours with 5 nM R1881 or 0.1% DMSO as vehicle control ATAC-seq was performed with 100,000 cells per treatment according to the Omni-ATAC-seq protocol [PMID: 28846090]. DNA was extracted using the DNA Clean & Concentrator Kit (Zymo research #D4014). Library amplification involved 11 PCR cycles using the NEBNext High-Fidelity 2x PCR Master Mix (New England Labs, Cat #M0541).