GSM5268477: E2; Rattus norvegicus; RNA-Seq
Total RNA of the Hypothermia Group (n = 5) and the Nomal Control Group (n = 5) were extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA). Agarose gel electrophoresis, NanoPhotometer spectrophotometer (IMPLEN, CA, USA) and RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA) were used for total RNA qualification. RNA samples with RIN ≥ 5.8 were select for sequencing. A total amount of 0.8 μg RNA per sample was used as input material for the library preparation. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations.