GSM4820773: Control-rep-1; Homo sapiens; RNA-Seq
Media was removed, 0.7 ml Qiazol (Qiagen) was added to each dish and the lysate was collected into Qiashredder tubes (Qiagen) and centrifuged at 12,000 x g for 30 seconds for homogenization. Total RNA was extracted using RNeasy kits (Qiagen) as recommended by the manufacturer. Total RNA concentrations were measured using Qubit reagents (Invitrogen). mRNA was enriched on oligo-dT beads, and reverse transcribed and sequenced using a PE150 protocol with Illumina reagents at the Novogene corporation.