RNA-seq of mus musculus: induced TH17 after HIF-1a inhibitor treatment
CD4+CD62L T cells were isolated from RBC-eliminated splenocytes of C57BL/6 mouse. These cells were cultured in TH17-skewing condition in 96 well culture plate coated anti-CD3e and anti-CD28 antibody. In the incubation period, the fusion proteins which inhibits endogenous HIF-1a were treated to compare the gene expression profile to non-treated group