GSM5461302: EPN_enriched_n3; Rattus norvegicus; RNA-Seq
At postnatal day 55, animals (N=3 per group) were euthanized by decapitation, brains removed fresh, sliced in a rat brain tissue matrix over ice, and CA1 of the hippocampus was quickly dissected from brain slices, frozen, and stored at -80ºC until used.  RNA was extracted manually using Qiagen RNeasy kits. Next generation sequencing libraries were prepared using the Illumina TruSeq Stranded Total RNA kit with ribosomal RNA depletion, following the manufacturer's protocol.