GSM4820777: Mmu_lps_bal_cells; Mus musculus; RNA-Seq
Bronchoalveolar lavage was performed by flushing lungs twice with 0.8 mL of Hanks solution containing 0.6 mM EDTA. BAL cells of eight animals were equally pooled for further analysis. Droplet-based single-cell partitioning of washed immune cells was performed using the 10X Genomics Chromium Controller. Single-cell RNA-Seq libraries were generated using the Chromium Single-Cell 3′ Reagent v2 Kit (10X Genomics, Pleasanton, CA) according to the manufacturer´s instructions.