GSM5395146: Multiplexed_sample_crispr_Day12 [LOU523A8]; Homo sapiens; RNA-Seq
Single cell mRNA-Seq with Feature Barcoding was carried out using the 10x Single Cell Gene Expression kit v3.1 according to the manufacturer's instructions. Briefly, cell suspension was counted and assessed for viability using an EVE automated cell counter (NanoEntek).  Approximately 10,000 cells were loaded into the 10x Chromium chip and loaded into the 10x.  GEM generation, barcoding, cDNA synesis and clean-up was carried out as per the 10x protocol.  Subsequently, the gene expression and feature barcoding libraries were separated by size selection and sequenced on the HiSeq 4000 according to the 10x guidelines. As per 10X Genomics' instructions 10X Genomics scRNAseq with sgRNA Feature barcoding