Illumina HiSeq 4000 sequencing; Transcription profiling of leaves of Populus nigra × P. maximowiczii growing in monoculture and stands mixed with Robinia at poor and rich soil sites
Transcription profiling of leaves of Populus nigra × P. maximowiczii growing in monoculture and stands mixed with Robinia at poor and rich soil sites
Mature leaves from the middle part of single trees were collected and immediately frozen in liquid nitrogen. Poplars were planted with stem cuttings at two field sites: Deppoldshausen (51.581°N/9.967°E) and Reinshof (51.484°N/9.923°E), Germany. Planting took place during April 2014. After growing for 4 years under ambient weather conditions, plants were harvested in 2018. Treatment conditions:a) The two field sites Deppoldshausen and Reinshof differ in soil nutrient levels. b) Poplars were planted in blocks, either as monoculture or in mixture with Robinia. mRNA was extracted using modified Trizol method (Chomczynski & Sacchi, 2006). cDNA libraries were prepared using the TruSeq RNA Library Preparation Kit from Illumina (New England BioLabs, Frankfurt am Main, Germany) according to manufacturer's protocol.