RNAseq of Mus musculus: adult male lumbar dorsal root ganglia
Lumbar DRG from control and MC38 tumor-bearing mice were collected after perfusion with PBS, and RNA was extracted using the RNeasy MinElute Cleanup Kit (Qiagen, Hilden, Germany), based on the manufacturers protocol, as described previously [44]. The prepared samples were then shipped on dry ice and the bulk RNA sequencing (mRNA library preparation) was performed by Novogene (California, USA; California Clinical Laboratory License No: 05D2146243), after ensuring the samples met or exceeded the proper quality control criteria (Agilent 2100 RNA integrity number for all samples >7)