NextSeq 550 sequencing; EZH2 rip-seq in mouse Th1 helper cells, resting and re-stimulated with pma/ionomycin
EZH2 rip-seq in mouse Th1 helper cells, resting and re-stimulated with pma/ionomycin
7 days differentiated Th1 cells were re-stimulated using pma/ionomycin. cells were washed and lysed using polysomal buffer for RNA immuno- precipitation.  Lysates were incubated with anti-EZH2 antibody over-night, magnetic beads were added, washed and RNA was extracted using 0.5% sds. RNA was purified using AMPURE xp-beads. Libraries were constructed using smarter stranded total RNA-seq v2 pico input.