GSM5461790: Cmlhp1abRNA-seq rep1; Cucumis melo; RNA-Seq
Total RNA were extracted from 1g of fresh leaves using the Nucleospin RNA plus kit (Macherey–Nagel), according to the manufacturer's instructions.  For RNA-seq analysis, libraries were synthetized using NEBNext UltraII Directional RNA library Preparation Kit (NEB) according to the manufacturer's instructions. RNA-seq libraries were prepared using 2 μg of total RNA with the NEBNext® Poly(A) mRNA Magnetic Isolation Module and NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (New England Biolabs), according to manufacturer's recommendations. The quality of the libraries was assessed with Agilent 2100 Bioanalyzer (Agilent).