RNA-Seq of Solanum betaceum Inoculated at 12 Hours Replicate 3
Total RNA was obtained from each sample using the RNeasy Plant Mini Kit (Qiagen, Germantown, MD, USA) according to the manufacturers instructions. Samples were enriched using poly(A) selection from the total RNA sample and then fragmented. cDNA library preparation was performed with the non-directional NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Hitchin, UK) and sequenced on an Illumina HiSeq4000 system (Illumina, San Diego, CA) over a paired-end library (insert size of 250-300bp) for each three biological replicates.