GSM4779213: Luminal2 [scATAC-seq]; Mus musculus; ATAC-seq
Mammary glands number 4 were collected and pooled from a total of four 10-week old, female FVB/NJ mice. Glands were minced into pieces ~1mm in diameter and were incubated with a 2mg/ml collagenase type IV solution at 37C while shaking for 1 hour. Digested organoids were collected by differential centrifugation. Collected organoids were further dissociated with trypsin into single cells. Cells were stained for flow cytometry using fluorescently labeled antibodies for CD49f, EpCAM, CD31, CD45, Ter119, and SytoxBlue. Basal and Luminal cells were sorted separately. Library generation for single cell ATACseq were performed following the Chromium Single Cell ATAC Reagent Kits User Guide: CG000168 Rev B. Libraries were sequenced on the Illumina HiSeq4000 platform targeting approximately 50,000 reads per cells.