RNA-seq of Radix Ardisia: fresh root
Total RNA was extracted with the RNAprep Pure Plant Kit (Tiangen, China) and detected on a 1% agarose gel. The purity, concentration, and integrity of the total RNA samples were assessed prior to further analysis. After the isolation and fragmentation of total RNA, mRNA was enriched by using poly-T oligo-attached magnetic beads. In this work, twelve cDNA libraries produced from the ACS and AC were sequenced by using the Illumina HiSeq 4000 platform and paired-end reads were generated, and clean reads were obtained by removing raw reads containing adapter, reads containing ploy-N and lower quality reads.