GSM5227386: fetalC2019_E3381-1_1000X2_MixN2018_Acute 48 hours; Homo sapiens; RNA-Seq
Total RNA was isolated with the RNeasy Micro Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. RNA was quantified with Nanodrop and then the integrity was evaluated with Agilent 2100 Bioanalyzer (only if the quality ratios were not optimal after Nanodrop analysis). TruSeq Stranded Total RNA LT Sample Prep Kit, Illumina was used to run the library for each sample. Sequencing is performed with the Illumina HiSeq 2000 platform, sequencing on average 10 million 50bp paired-end reads per sample.