Metagenome of Hot spring sediment metagenome/environmental sample from Radiata Pool
DNA from Radiata Pool hot spring sample P10019 was extracted from approximately 10g of sediment material using FastDNA 50ml spin kit for soil (MP Biomedicals) spiked with 400l of PolyA. The DNA eluted in 5ml of water was further cleaned using the Aurora system. DNA concentrations at various stages of extraction and library construction were measured with ND-3300 fluorescent Nanodrop instru-ment (Thermo Scientific). Nextera DNA library preparation kit was used. Metagenomic sequence data was generated with Illumina HiSeq 2500 instruments at Uppsala SNP&SEQ Technology Platform.