Blood group typing from whole-genome  sequencing
Library preparation was performed using the  TruSeq Nano DNA LT kit (Illumina, San Diego, CA) according to the manufacturers recommendations. Library size  and quality was confirmed with Fragment Analyzer (Advanced Analytical, Santa  Clara, CA) and quantitation was done using qPCR (S1000/CFX96 Real Time  System; Biorad, Hercules, CA) Paired-end sequencing (2x150bp ) was performed  on the NovaSeq 6000 System (Illumina) following the manufacturers  recommendations.