GSM5416441: ASXL1-MT-KI1; Mus musculus; RNA-Seq
We extracted total RNA using the RNeasy Micro Kit (QIAGEN) from bone marrow LK cells derived from 8- to 12-week-old littermate wild-type and ASXL1-MT-KI mice. The synthesis and ampliﬁcation of cDNA were performed with 200 ng RNA using the NEB Next Ultra RNA Library Prep Kit for Illumina (New ENGLAND BioLabs：E7530), followed by high-throughput sequencing on the HiSeq 2000 System (Illumina) with 124 bp paired-end reads.