GSM5599197: Patient_Tcell_21hrStim_rep3; Homo sapiens; RNA-Seq
RNA was isolated using the RNeasy Plus Micro Kit (Qiagen) Reverse transcription and cDNA pre-amplification were performed using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech/Takara). Libraries were prepared using the Nextera XT DNA Sample Preparation Kit and the Nextera Index Kit (Illumina). mRNA libraries were pooled and paired-end 150-bp sequencing was performed on the Illumina HiSeqX platform.