GSM6165464: Stable Vitiligo PtE nonlesional; Homo sapiens; RNA-Seq
All procedures were conducted according to an approved institutional review board protocol of the University of California, Irvine (HS No. 2018-4362), with written informed consent obtained from all patients. The donor skin sites were cleaned with ethanol wipes and 5 suction blisters (1cm diameter) were created by applying a standard suction blister device. We unroofed the blisters and used half for melanocyte-keratinocyte transplant procedure(67). The rest of the blisters were incubated in trypsin for 15 minutes at 37°C, followed by mechanical separation and centrifugation at 1000 rpm for 10 minutes at 4°C to pellet cells. Cells were washed with 0.04% UltraPure BSA:PBS buffer, gently re-suspended in the same buffer, and filtered through a 70mm mesh strainer to create a single cell suspension. Cells were washed and viability was calculated using trypan blue Library was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics).