ITS metabarcodes:Gnatotrichus materiarius
In the laboratory, the specimens were rinsed with pure water to remove loosely adhering fungal tissue, and thoroughly macerated individually to ensure that all fungi associated with the specimens were released. DNA was extracted using the Qiagen DNeasy Blood and Tissue spin column extraction kit (Qiagen, Valencia, CA, USA). The DNA extracts were then used for fungal metabarcoding of ITS with primers ITS86F (5-GTGAATCATCGAATCTTTGAA-3) / ITS4 (5-TCCTCCGCTTATTGATATGC-3) (Op De Beeck et al., 2014).Raw reads were demultiplexed, primer trimmed and singleton reads removed with Cutadapt (Martin, 2011).