GSM4878990: MFP_3; Homo sapiens; RNA-Seq
The metastatic lesions were dissected with ex vivo BLI imaging, and the uninvolved tissues were removed in the hood. The surgery tools were sterilized with a bead-sterilizer following by 70% isopropanol between different animals. The collected tissues were immediately subjected to the tumor dissociation kit and RFP positive cells were sorted and re-cultured in vitro. The total RNA were extracted using TRIZOL with directzol RNA isolation kit following the manufacturer's protocol. the library construction was performed by Novogene.