SIT55616RN
Genomic DNA was extracted from the maternal parent FD061129(BM4), paternal parents SIT55616RN(GF2) using DNA extraction kit(Geneall, Seoul, Korea) from young leaves collected during the fruit harvesting period. The extracted DNA was evaluated using a fluorometer and diluted to a concentration of 100 ng/L for NGS. Raw reads were generated using paired-end sequencing (2  100150 bp) with the Illumina HiSeq 2000 and NextSeq platforms. As a pre-processing step, only reads with a minimum length of >25 bp and Phred score >20 were filtered using DynamicTrim and LengthSort of SolexaQA (version.1.13). The selected reads were mapped to the watermelon 97103 genome assembly version 2 using BWA (0.6.1-r104).