GSM6329503: Wt Intact repl.2; Danio rerio; RNA-Seq
Telencephali were extracted and dissociated into single cell suspension using the papain kit (Worthington), according to manufacturer´s instructions Single-Cell RNA Sequencing libraries were prepared according to Chromium Single Cell 3´Reagent Kit v3 and v3.1 (10X Genomics) and sequenced on NovaSeq6000 (Illumina) with recommended number of cycles (28-8-91) for sample 8 and (28-10-10-90) for samples 9-12.