GSM5549078: WT_Bl6_mouse_Naive-1; Mus musculus; RNA-Seq
sCells were cultured maintained in DMEM (Corning, 15-013-CV) complemented with 10% FBS (GIBCO by Life Technologies, A31605-01) and 1% Penicillin-Streptomycin (GIBCO by Life Technologies, 15140-122). Dissociation of tissues into single cells: All samples were collected in Media 199 supplemented with 2% (v/v) FBS. Single cell suspensions of the tumors were obtained by cutting the tumor in to small pieces (1mm3) followed by enzymatic dissociation for 45 minutes at 37°C with shaking at 120 rpm using Collagenase I, Collagenase II, Collagenase III, Collagenase IV (all at a concentration of 1 mg/ml) and Dispase (2mg/ml) in the presence of RNase inhibitors (RNasin (Promega) and RNase OUT (Invitrogen)). Erythrocytes were subsequently removed by ACK Lysing buffer (Quality Biological) and cells resuspended in Media 199 supplemented with 2% (v/v) FBS for further analysis.