NP free study on Zebrafish
Palladium nanoparticles (Pd NPs) have been the reliable substitutes as conventional catalysts for its high efficiency and conversion of CO, hydrocarbons, and NO into less harmful CO2, water and nitrogen. Due to surface abrasion of converters, Pd as ultrafine/nanoparticles are liberated into the environment causing possible health effects. Also, Pd might reach the aquatic system through heavy precipitation and runoff. Here, we aimed to profile the transcriptome of Danio rerio exposed to nano-palladium at environmentally relevant concentrations with control. 500 ng of total RNA of nano-Pd free fishes (Control) was isolated and the enriched mRNAs were reverse transcribed to form cDNA. The purified amplified cDNA library was eluted in the TE buffer. The concentrations above 2 ng/µL were used for the transcriptome analysis with the reference genome. In the control group, 67835 genes were expressed which showed no changes in their behavior.