GSM5515763: Control Sample 1; Homo sapiens; RNA-Seq
Neutrophils were isolated using MACSxpress Whole Blood Neutrophil Isolation Kit in combination with MACSxpress Erythrocyte Depletion Reagent (Miltenyi Biotec, Bergisch Gladbach, Germany). For isolation of RNA, we used the RNAeasy Mini kit from Qiagen. Beta-mercaptoethanol was added to RLT buffer as mentioned in the manufacturer's protocol. Sequencing libraries were generated using 200 ng of RNA and the TruSeq Stranded mRNA Kit according to manufacturer's instructions (Illumina, San Diego, USA).