Illumina HiSeq 2000 sequencing; Influence of CAP-D3 in Arabidopsis thaliana transcription
Influence of CAP-D3 in Arabidopsis thaliana transcription
Four-week old plantlets were collected by cutting above the root (all aerial part was used) and froze directly in liquid nitrogen.  Plants were grown in soil under short day conditions (16h dark/8h light) at 18-20 C 50 mg of frozen pooled plant material was grounded and total RNA isolated with RNeasy Plant Mini Kit following manufacture's instructions. TruSeqRNA (180 bp insert) and ERCC 1&2 spike-in