COLON
The small and large intestinal contents of MAT PE mice were collected at day 14 after MAT PE. Bacterial DNA was extracted using the E.Z.N.A. soil DNA Kit (Omega Bio-tek, Norcross, GA, U.S.) according to manufacturers instructions. The hypervariable region V3-V4 of the bacterial 16S rRNA gene were amplified with primer pairs 338F (5'-ACTCCTACGGGAGGCAGCAG-3') and 806R(5'-GGACTACHVGGGTWTCTAAT-3'). 16S rRNA gene amplicons were generated and sequenced on Illumina MiSeq PE300 platform according to the standard protocols by Majorbio Bio-Pharm Technology Co. Ltd. (Shanghai, China).