WGS of Lupinus angustifolius: cultivar Geebung
The sequencing libraries were generated using NEBNext DNA Library Prep Kit (Illumina) following the manufacturers recommendations and indices were added to each sample. The gDNA was randomly sheared to produce 350 bp fragments that were then end-polished, A-tailed, ligated with the NEBNext adapter for Illumina sequencing, and further PCR enriched by P5 and indexed P7 primers. The PCR products were purified (AMPure XP system) and the resulting libraries were analysed for size distribution by Agilent 2100 Bioanalyzer and quantified using real-time PCR. Whole genome sequencing (WGS) was conducted at approximately 10x coverage using Illumina sequencing by synthesis technology.