Illumina NovaSeq 6000 paired end sequencing; RNA-Seq analysis of E18.5 mouse Zfp800 knockout vs wild-type
RNA-Seq analysis of E18.5 mouse Zfp800 knockout vs wild-type
Embryos from Zfp800 het x het mouse maitings were collected at embryonic day E18.5.  Embryos were genotyped by PCR and pancreata from wild type and Zfp800 knockout mouse embryos were dissected out for RNA islolation. All mice used for experiments were on C57Black/6J background.   Wild type and Zfp800 knockout embryonic  pancreata were used in the experiment. Total RNA was extracted from  mouse pancreatic tissue using the Maxwell simplyRNA kit (Promega).  cDNA libraries were constructed using  Illumina library prep kit at Novogen.