Cas9 Knockout Screen on HIV-1 infected human CEM-SS T cells
Whole genome CRISPR knockout screen using the Brunello sgRNA library was carried out to detect genes that are essential in silencing unintegrated HIV-1 DNA. We sequenced sgRNAs from infected CEM-SS T cells from all cells and from only the GFP+ cells and looked for sgRNAs that were enriched in the GFP+ cells.