GSM6267980: B1b_WT_2; Mus musculus; RNA-Seq
Extraction and purification of RNA were performed using RNeasy kit (Qiagen). Total amount of ~200ng for each sample was used to construct sequencing libraries. All of the library preperation steps were performed by Novogene. In brief, mRNA was enriched with poly A capture following by RNA fragmentation using enzyme digestion and cDNA generation using reverse transcription.