GSM5550176: HNF4A overexpressing stomach organoids CDX2 3' enhancer homozygous deletion rep 1; Mus musculus; RNA-Seq
Orgnaoids were removed from matrigel using cell recovery solution and immediately resuspended in trizol for RNA extraction. Orgnaoids were removed from matrigel using cell recovery solution and immediately resuspended in trizol for RNA extraction. RNA was extracted using Qiagen miniRNA kit with additional on column Dnase digestion. Libraries were made using NEB Poly-A library preparation kit and 150bp paired end sequencing was performed on Illumina platform.