Genomic sequencing of SARS-CoV-2: human clinical sample
Viral sequencing was performed following a tiling amplicon strategy using the ~ 1200bp primer scheme by Freed et al. (2020). All sequencing was performed using Oxford Nanopore sequencing platforms using R9.4.1 flow cells with the GridION. Libraries were prepared via the ligation sequencing kit (SQK-LSK109) and multiplexed using barcode expansion kits (NBD104,114). All data was de-hosted using nanostripper and re-basecelled with Guppy 4.0.11