Single nuclei RNA sequencing of patient sample: Sample MBM17_sn
Single-nuclei samples: Single-nuclei suspension from frozen melanoma metastasis were generated using methods published in Slyper et al. (Nature Medicine, 2020) with modifcations. Salt-tris buffer with Tween-20 (TST) was used for dissociation followed by a high-volume wash in salt-tris buffer (ST). Nuclei were counted and 15,000 nuclei were separated for droplet-based RNA capture and reverse transcription using 10x Genomics  3' v3.0 reagents and a Chromium controller.