GSM5822429: Multiplexed sorted BM Lin-CD127+ cells; Mus musculus; RNA-Seq
For BM sinusoid cell labeling, WT or Plzf-GFP mice were injected i.v. with anti-CD45.2- AmCyan antibody (1 µg, clone 104, BioLegend). Mice were sacrificed 2 minutes after the injection and BM cells were stained for flow cytometry to determine cell numbers of indicated ILC progenitor populations. cDNA synthesis was performed using the BD Rhapsody Express Single-Cell Analysis System, and gene expression libraries were prepared using Whole Transcriptome Analysis Amplification Kit (all from BD Biosciences). Whole Transcriptome Analysis Amplification Kit (all from BD Biosciences).