GSM5548829: K177Q-METTL3 reconstituting MDA-MB-231 polysome rep2; Homo sapiens; RNA-Seq
Collected fractions were pulled into sub-polysome fraction and polysome fraction. Total RNA was isolated from samples with TRIzol reagents following the manufacturer's instructions. Poly(A)-selected mRNAs were purified and used for library construction using NEBNext® Ultra™ Directional RNA Library ep Kit for Illumina®. Sub-polysome and polysome samples were subjected to RNA-seq.