cDNA Seq of Zika virus
38 ZIKV-specific binding primers were made based on the ZIKV reference genome. RNA was extracted from the patient's specimen, which then was used for the synthesis of cDNA. With already-made primers and cDNA, multiplex PCR was performed so that sequences related to ZIKV can be amplified. After the quantification of amplicons, library preparation and sequencing was performed by Ligation Kit and MinION (Oxford Nanopore Technologies).