miRNA-Seq of Meloidogyne graminicola: second stage juveniles
Small RNAs (18~30 nt) were isolated by the denaturing PAGE method of Lagos-Quintana et al.(Lagos-Quintana, Rauhut et al. 2001). Agilent 2100 Bio-analyzer (Agilent Technologies, Inc) was used to assess RNA quality, only samples with RIN (RNA integrity number) greater than eight in downstream experiments. Small RNA sequencing libraries were then constructed using the TruSeq Small RNA Library Preparation Kit (Illumina, San Diego, CA, USA). Library sequencing was performed by Illumina HiSeq 2000