HiFi reads from Aldabrachelys gigantea
The PacBio HiFi library was produced using the SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences), according to the manufacturers instructions. Briefly, 14 g HMW DNA sample was mechanically sheared to an average size of 20 Kbp. The sheared DNA sample was DNA damage repaired and end-repaired using polishing enzymes. PacBio sequencing adapters were ligated to the DNA template. A Blue Pippin device (Sage Science) was used to size-select fragments >15 Kbp. The SMRT bell-Polymerase Complex was prepared using the Sequel II Binding Kit 2.0 and Internal Control 1.0 (Pacific Biosciences) and sequenced on a PacBio Sequel II instrument using the Sequel II Sequencing Kit 2.0 (Pacific Biosciences). In total, two Sequel II SMRT Cells 8M (Pacific Biosciences) were run, taking one movie of 30 hours per cell.