Illumina NovaSeq 6000 sequencing; Spatial transcriptomic reference of the endometrium and myometrium of women in reproductive age
Spatial transcriptomic reference of the endometrium and myometrium of women in reproductive age
Human tissue was obtained from deceased transplant organ donors after ethical approval (reference 15/EE/0152, East of England—Cambridge South Research Ethics Committee) and informed consent from the donor families. Uterus was excited within 60 min of circulatory arrest and stored in saline (HypoThermosol biopreservation media) at 4 °C until processing. Tissue was embedded with OCT and flash-frozen using isopentane: https://www.protocols.io/view/embedding-and-freezing-fresh-human-tissue-in-oct-u-95mh846 Tissue was analysed according to the manufacturer's protocol for Visium (10x Genomics) Library preparation was carried out according to the manufacturer's protocol for Visium (10x Genomics)