GSM6268516: DUAN2; Capra hircus; RNA-Seq
The experiment population consisted of a polytocous group (n=7, litter size≥ 3) ——Nubian goat and a monotocous group (n=7, litter size=1) ——Du'an goat based on three lambing records. The animals were maintained at Standardized sheep breeding farm. Without any other extra exogenous hormones that may induce superovulation in goat, all goats treated with controlled internal drug releasing (CIDR, progesterone 300 mg) for 17days to achieve synchronous estrus. Ovarian tissue samples from 2 Nubian goats (about 3 years old) and 2 Du'an goat (about 3 years old) were collected and processed for snRNA-seq analysis. Single cells were suspended in calcium- and magnesium-free PBS containing 0.04%weight/volume BSA. RNA libraries were prepared with Chromium Single cell 3' Reagent v3 Kits according to the manfacturer's protocol.