GSM6268624: LCS7846_GV_WT2_M_CleanData; Mus; miRNA-Seq
RNA extraction with qiagen miRNeasy kit Library preparation and microRNA sequencing was performed by LC Sciences according to the following criteria. Total RNA was extracted from cerebellum of WT and Pum1-/- male and female at 10 weeks of age using the miRNeasy kit (QIAGEN, # 217004) according to the manufacturer's instructions. The total RNA quality and quantity were assessed with Bioanalyzer 2100 (Agilent Technologies, Santa Clara) with RIN number > 7.0. Approximately 1 µg of total RNA were used to prepare the small RNA library according to the protocol of TruSeq Small RNA Sample Prep Kits (Illumina, San Diego). Then the single-end sequencing 50bp was performed on an Illumina Hiseq 2500 at LC Sciences (Hangzhou, China) following the vendor's recommended protocol.