GSM5515082: NSC_ZD7288_treatment_I; Rattus norvegicus; RNA-Seq
Cells were pipetted into a 5,184 well chip (Icell8 platform, Wafergen / Takara), and after life/dead staining 500 wells containing single living cells (and 10 control wells) were selected for in well cDNA synthesis and cDNA was collected with collection kit (WaferGen / Takara). WaferGen / Takara protocol: SMARTScribe RT cDNA synthesis, SeqAmp cDNA amplification, concentrated with DNA Clean & Concentrator Kit (Zymo Research) and purified with 0.6X AMpure XP beads (Beckman). After QC on Tape 12 Station (Agilent) cDNA was tagmented (Nextera XT transposome) and adaptor sequences added. Library purification was performed with AMpure XP beads, followed by dual SPRI treatment.