GSM6032225: Control_1; Mus musculus; RNA-Seq
3000 lung ILC2s (CD3−CD4−CD8−CD11b−CD11c−CD19−Gr-1−B220−CD5−FceRⅠ−CD45−Ter119) were sorted by BD LSR Fortessa, and cells were lasated by Buffer RLT (QIAGEN). Then total RNA was harvested using Agencourt AMPure XP (Beckman Coulter) size selection beads. Takara SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Cat#634890) was used with all extracted RNA for cDNA library.  Illumina Nextera XT DNA Library Preparation Kit (Cat#FC-131-1024) and Nextera XT Index Kit (Cat#FC-131-1001) were used with 1 ng of cDNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols