NGS of S. oneidensis:EW1
pEWTEST1 with a string of repetitive C was introduced as target to test the editing window of base editors with 20bp-spacer. pEWTEST1 was transformed into S. oneidensis MR-1 already harboring pCYR104 (dCas9-AID) by another round of conjugation. The cells were collected and spread on LB plates with Km and Cm. Two colonies were picked, inoculated and induced in LB with Km, Cm and IPTG for 24 h. The bacteria plasmids were extracted and the target range was amplified and then NGS sequenced.