GSM5198805: HNRNPDL knockdown replicate 1; Homo sapiens; RNA-Seq
Total RNA from HUVECs was isolated 48 h later, using the miRNeasy Mini kit (Qiagen), including the optional on-column DNA digest with the RNase-Free DNase Set (Qiagen). After isolation, 500 ng RNA were quality checked on a 1% agarose gel. For the HNRNPDL knockdown experiments (n = 2), total RNA from HUVECs transfected either with a non-silencing control siRNA (si_ctrl) or an siRNA targeting HNRNPDL (si_DL) was used to generate polyA-enriched strand-specific cDNA libraries. Libraries were sequenced on a HiSeq 2000 (Illumina), obtaining 76 nt paired-end reads.