RNA-Seq of rattus norvegicus: adult female bone marrow stem cells
Total RNA was isolated using the RNeasy Plus Mini Kit (Qiagen) according to the manufacturer's instructions. The purity and concentration were measured using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies LLC). RNA integrity was assessed by a BioAnalyzer (Agilent Technologies). The mRNA libraries were generated using NEBNext Ultra II Directional (stranded) RNA Library Prep Kit for Illumina (NEB #E7760L) and sequencing was performed on the Novaseq 6000 system with the SP flowcell and 150bp PE reads.