GSM6031755: CF_LPS, scRNAseq; Homo sapiens; RNA-Seq
Single-cell suspensions of basal cell culture were prepared by lifting adherent cells using trypsin digestion. Dead cells were removed using the MACS Dead Cell Removal kit (Miltenyi Biotech). Cells were then washed and counted as per manufacturer's instructions libraries were prepared using the 10X NEXT GEM 3' Gene Expression Library scRNA Seq v3.1 reagents and workflow (10x Genomics). Library concentration and quality was confirmed by BioAnalyzer.