GSM5417471: Lymph_RNA; Mus musculus; RNA-Seq
Single cell suspensions of freshly isolated gd T cells from lymph, spleen and thymus by MACS and FACS were resuspended in PBS containing 0.04% ultra-pure BSA, and sorted gd T cells were diluted to <1000 cells/μL; Isolation, washing and counting of nuclei suspensions were performed according to the Demonstrated Protocol: Nuclei Isolation for scATAC-seq (10×Genomics). According to the manufacturer's protocol, single cell sequencing libraries were prepared using the Chromium Single Cell 3' Reagent Kits v2 (10×Genomics). Indexed libraries were further sequenced using an Illumina NextSeq550 system in pair-end mode; scATAC-seq libraries were prepared according to the Chromium Single Cell ATAC Reagent Kits User Guide (10×Genomics; CG000168 Rev B)