ATAC-Seq of Bicyclus anynana: 5th instar prolegs
Prolegs were dissected from late 5th instar larvae. 20 individuals (40 prolegs) were dissected for each replicate. Samples were snap frozen immediately using liquid N2 and stored in -80C until further use. ~80,000 nuclei were used for each library. Nuclei isolation and library preparation were done with little modification as described in Omni-ATAC protocol (Corces et al, 2017). Sequencing was performed at AIT Novogene, Singapore; Reads from the sample were aligned to the Bicyclus anynana (BaGv2) genome using bowtie. Peak call was done using F-seq.