GSM5227210: Fusobacterium nucleatum+metformin 1mm rep_1; Fusobacterium nucleatum subsp. nucleatum ATCC 25586; RNA-Seq
Total RNA of F. nucleatum was stabilized with RNA protect Bacteria Reagent (QIAGEN, Germany) and extracted using a QIAGEN RNeasy kit (QIAGEN, Germany) following the manufacturer's instructions. Any remaining genomic DNA was digested by On-column RNase-Free DNase kit (Qiagen, Germany). Ribosomal RNA removal, cDNA library construction, and paired-end sequencing with the Illumina HiSeq 2000 were completed by Guangdong Magigene Biotechnology Co. Ltd.