DNA-PacBio
The genomic DNA of ACB was extracted from the pupae using an AxyPrep Multisource Genomic DNA Miniprep Kit (Axygen, Union, CA, USA) according to the manufacturers instructions. A 20-kb SMRTbell template library was prepared using the SMRTbell Express Template Prep Kit 2.0 (PacBio, #100-938-900) from a single male pupa of F colony and then the size was selected by Blue Pippin (Sage Science, Beverly, MA, USA). Whole genome sequencing was performed commercially (Sinobiocore, Beijing, China) on a PacBio sequel II (Pacific Biosciences, Menlo Park, USA) system using V3 polymerase according to the manufacturers instructions.