FACS-sorted single cell RNA sequencing of patient sample: Sample MBM05_sc_CD45pos
Single-cell samples: Melanoma brain metastasis from surgical resections were dissociated using the human tumor dissociation kit from Miltenyi and viable CD45- and CD45+/CD15-/CD66b- populations were sorted. Of each sorting population 15,000 cells were loaded for droplet-based RNA capture and reverse transcription using 10x Genomics 5' reagents and a Chromium controller. TCR libraries were constructed using TCR amplification reagents from 10x Genomics according to manufacturer instructions.