RNA-Seq of Freezing and thawing soil
DNA was extracted using a Fast DNA SPIN Kit for Soil and a Fast Prep instrument (MP Biomedicals, California, USA) according to the manufacturer's instructions. The DNA quality was assayed on a 1% agarose gel and the DNA quantity was determined with a Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA). Then V3-V4 hypervariable region of the 16S rRNA gene was then amplified by PCR. A MiSeq 300 Cycles Kit was used to sequence 2  150 bp paired-end reads on the Illumina MiSeq platform (Shanghai Shenggong Illumina MiseqTM), following constructs bacterial 16S rDNA amplification sublibrary