GSM5550183: ABCtr-019 (control) single B cells, scV(D)J-seq; Homo sapiens; OTHER
Frozen human PBMCs were rapidly thawed in a 37ºC water-bath and dead cells were removed with the Dead Cell Removal kit and an AutoMACS Pro Separator (both from Miltenyi Biotech). Untouched B cells were then isolated with the B cell Isolation kit II (Miltenyi Biotech) and resuspended in PBS, 0.04% BSA at 7-11x105 cells/ml. The Chromium Next GEM Single Cell 5' Library and V(D)J Enrichment Kit for Human B Cells (version 1.1) was used to construct V(D)J-enriched libraries (following manufacturer's instructions) scV(D)J-seq