Illumina NovaSeq 6000 paired end sequencing; Cellular and extracellular vesicle RNA sequence for E-cadherin-RFP EMT reporter system expressing mouse breast cancer cell line Py2T during EMT/MET
Cellular and extracellular vesicle RNA sequence for E-cadherin-RFP EMT reporter system expressing mouse breast cancer cell line Py2T during EMT/MET
EVs were collected by ultracentrifugation, 120,000 x g, 90 min, 4ºC 0%FBS/DMEM 5 ng/ml TGFbeta1 TRIzol, PureLinkRNA Mini kit Illumina TruSeq® Stranded Total RNA Library Prep (Human/Mouse/Rat ) kit