GSM4728381: D25 t0h; Homo sapiens; miRNA-Seq
Samples were lysed in QIAzol Lysis Reagent and RNA was extracted using the miRNeasy Mini Kit. RNA integrity was evaluated using an Agilent 2100 Bioanalyzer. 200 ng of total RNA were used to generate barcoded miRNA-seq libraries using the NEBNext Multiplex SmallRNA Library Prep Set from Illumina. The quantity and quality of the miRNA libraries were determined using the Agilent 2100 Bioanalyzer High Sensitivity DNA chip.