SLAF-seq of muscle: adult Pampus echinogaster
According to the genome size and guanine+cytosine content of P. argenteus, enzymatic digestion prediction was performed. The analysis software for SLAF enzymatic digestion prediction independently developed by Biomarker Technologies Corporation was used to predict the digestion of the reference genome, and the optimal digestion scheme was selected according to the following principles: the percentage of enzymatic fragments located in the repeat sequences was as low as possible; the enzymatic fragments were distributed as evenly as possible across the genome; the length of each enzymatic fragment was consistent with that of the specific experimental system; and the number of obtained enzymatic fragments agreed with the expected number of tags. With the HaeIII restriction enzyme, the genomic DNA of each qualified sample was digested separately. Each obtained digestion fragment had a poly A tag added to the 3' end, the dual-index sequencing adaptors were attached, and this new sequence was amplified by polymerase chain reaction, purified, and mixed. The target fragments were taken out by cutting the gel. After the samples were qualified by the library, they were sequenced using the Illumina HiSeq 2500 platform. To evaluate the accuracy of enzyme digestion, Nipponbare was selected as the control for sequencing.