GSM6032122: Endometrium, r-101; Bos taurus; RNA-Seq
RNA extraction was performed using RNeasy Mini Kit (Qiagen, Valencia, CA). RNA concentration and quality were determined using spectrophotometry (NanoDrop 2000 Spectrophotometer, Thermo Fisher Scientific, Waltham, MA, USA) by absorbance at 260 nm and the 260/280 nm ratios, respectively. Absorbance ratios ranged from 1.8 to 2.1. All the RNA-sequencing procedures were performed by Novogene Bioinformatics Technology Co., Ltd (Beijing, China). Sequencing libraries were prepared using a Poly-A tail capture method and sequenced with NovaSeq 6000.