GSM5195799: Photoreceptor_RNA-seq_Rep1; Drosophila melanogaster; RNA-Seq
Male flies were harvested at indicated time point and heads were removed by freezing the flies in liquid nitrogen followed by five rounds of vortexing and freezing. RNA was extracted from isolated GFP-tagged photoreceptor nuclei using the method described in this manuscript ChIP-seq libraries were made with Nugen Ovation Ultralow V2 using 2 ng of DNA as starting material. CUT&Tag librares were made following Epicypher protocol, including the IDT adapters, RNA-seq libraries were made with Nugen SoLo RNAseq system using 10 ng RNA as starting material. ATAC-seq libraries were made using IDT for Illumina primers Set A