GSM5351478: D016.19; Homo sapiens; RNA-Seq
These samples came from 23 individuals aged 73-84 years old, who died of respiratory or heart failure, or from a myocardial infarction. The human donor eyes were obtained with the Utah Lions Eye Bank. We include samples within 6 hours post-mortem for this study. Dissections of donor's eyes were carried out immediately according to a previously published protocol (Owen et. al., Investig. Ophtalmol. Vis. Sci., 2019). Specifically, the macula retina tissue was collected using a 6 mm disposable biopsy punch (Integra, Cat # 33–37) and flash frozen and stored at −80 °C. Donors with any history of retinal degeneration, diabetes, macular degeneration, or drusen were not used for this study; also each donor underwent an ophthalmology check to ensure the eye is in healthy condition. Matched samples with the snRNAseq were used for RNA isolation by applying TRIzol (Invitrogen) to the separated single-nuclei resuspension. cDNA were prepared from ∼1ng of total RNA by using the Smartseq v4 ultra low input RNA Kit according to the manufacturer's directions (Takara). Libraries were made using Nextera XT library prep (Illumina).