GSM5643683: X33C2; Mus musculus; RNA-Seq
Kidneys were harvested and frozen at -80C. RNA was isolated using NEXTflex Poly(A) Beads (Bioo scientific, Austin, TX). Libraries were prepared using the Rapid Directional RNA-Seq Kit (NEXTflex) RNA libraries were prepared for sequencing using the Rapid Directional RNA-Seq Kit (NEXTflex), looped in equimolarly and sequenced on Illumina NextSeq500 to produce single-end 75 base long reads