Panicum root
A total of 50ng amplicon DNA from the sample was end-repaired, cleaned up with 1X AmPure beads. Barcode adapter ligation was performed with NEB blunt/TA ligase and cleaned with 1X AmPure beads. Qubit quantified adapter ligated DNA sample were barcoded using PCR reaction and cleaned up with 1.6x AmPure beads. Qubit quantified barcode ligated DNA sample were pooled at equimolar concentration and end-repair was performed using NEBnext ultra II end repair kit. End-repaired DNA was cleaned up with 1x AmPure beads. Adapter ligation was performed for 15 minutes using NEB blunt/ TA ligase. Library mix was cleaned up using Ampure beads and finally eluted in 15 l of elution buffer