Genome skimming: Astragalus arrectus - LH 2931
Fragmentation with Bioruptor Pico sonicator using 5 cycles of 15 s On/90 s Off; NEBNext Ultra II DNA Library Prep Kit with no size selection and half volumes, 6 PCR cycles using NEBNext Dual Index Primers i709 and i506; Index Read 1: CGGCTATG, Index Read 2: TAAGATTA