GSM5550044: Sample 2; Homo sapiens; RNA-Seq
Total RNA from cell lines was purified with the Maxwell 16 Total RNA Purification Kit (Promega) . For RNA-Seq we used the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs Inc., Ipswich, MA, USA) and the NEBNext Multiplex Oligos for Illumina (New England BioLabs Inc.) for cDNA synthesis and addition of barcode sequences. The pre-pools sequenced were performed using the NextSeq 500 (Illumina, San Diego, CA, USA) with the NextSeq 500/550 High Output Kit v2.5 (75 cycles; Illumina). Stranded, single-ended 75bp-long sequencing reads.