GSM5776746: 6 weeks after cercarial infection [TCR]; Mus musculus; RNA-Seq
Mice were sacrificed under anesthesia and spleens were harvested. Spleen tissue was minced in a sterile Petri plate. The erythrocytes were lysed in ACK buffer and a single-cell suspension of murine splenocytes was prepared by passing cells through 70 μm strainers. Single cell RNA libraries were prepared using Chromium Single cell 5' Library & gel bead kit and coupled Chromium single cell V(D)J enrichment kit&scT/BCR library according to the manufacturer's protocol