Novel Actinobacteria species isolated from Trondheim fjord, Norway
The data collected from the PacBio Sequel instrument was processed and filtered using the SMRT Link software suite. Subreads shorter than 50 bp were discarded. The quality of Illumina reads was improved by trimming off low quality bases using BBDuk, which is a part of the BBMap suite version 36.77. High-quality reads were assembled into contigs using ABySS version 2.0.2. The long reads were mapped to the draft assembly using BLASR version 1.3.1. Based on these alignments, the contigs were linked together and placed into scaffolds. The orientation, order, and distance between the contigs were estimated using SSPACE-LongRead version 1.0. Using Illumina reads, gapped regions within scaffolds were closed using GapFiller version 1.10. Finally, assembly errors and the nucleotide disagreements between the Illumina reads and scaffold sequences were corrected using Pilon version 1.21.