GSM5550166: 759_cy7_microglia; Mus musculus; RNA-Seq
Total RNA was extracted from FACS-isolated microglia and astrocytes using miRNeasy Micro Kit (Qiagen, 1071023), following the manufacture's instructions. During the purification, DNase was utilized to ensure the total RNA was free of genomic DNA. After determining the integrity on an Agilent Bioanalyzer, extracted total RNA was prepared using the Takara-Clontech SMARTer library preparation method in the GTAC at Washington University.