GSM4822544: Cross B F3 susceptible SRP1; Sisymbrium orientale; RNA-Seq
Samples were frozen immediately in liquid nitrogen and total RNA was extracted using the RNeasy Plant Mini Kit (Qiagen). Sample quality was assessed by RIN score (Agilent TapeStation) as well as by gel electrophoresis. DNase treatment, library preparation, and sequencing were conducted at the Roy J. Carver Biotechnology Center at the University of Illinois. Libraries were prepared using the TruSeq Stranded mRNA Sample Prep Kit (Illumina).