GSM6031789: Overexpression-WalR-D54E_biol rep2; Clostridioides difficile; RNA-Seq
Cultures were fixed by mixing with an equal volume of cells with ice-cold 1:1 ethanol:acetone. Cells were lysed with a FastPrep-24 homogenizer and RNA was extracted with the Qiagen RNeasy kit. All samples had a RIN of 9.4 or higher (Agilent Bioanalyzer). Illumina Stranded RNA library preparation paired with RiboZero Plus  was prepared per manufacturer's specifications.