GSM5583192: RRP6KD-sonic-2; Homo sapiens; RNA-Seq
Harvested cells were lysed in NP-40 containig buffer and nuclei were collected by centrifugation. Total nuclear RNAs were prepared with Sepasol-RNA I Super G (Nacalai Tesque) either by hp-method or by sonic-method. RNA libraries were prepared with QuantSeq 3'mRNA-Seq Library Prep Kit (FWD)(LEXOGEN) according to the manufacture's instructions for sequencing using standard Illumina protocols.