GSM6165355: wt-replicate NoD [NoD_BH4]; Homo sapiens; ncRNA-Seq
Total RNA was extracted from cells. Small RNA was size-selected and treated or not treated with NaBH4+Aniline (that induces RNA chain cleavage at methylated guanosines). Sequencing libraries were prepared following the protocol included with the kit “NEXTflex™ Small RNA-Seq Kit v3,” (©Bioo Scientific Corp. Catalog #5132-05).