GSM5227641: Sample4; Bos taurus; RNA-Seq
RNA extraction was performed using RNeasy Mini Kit (Qiagen, Valencia, CA). RNA concentration and quality were determined using Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA) and Qubit 2.0 Fluorometer (Thermo Fisher Scientific Inc, Waltham, MA). Bioanalyzer and Qubit RNA integrity numbers ranged between 5.6 and 8.7, and between 6.9 and 9.4, respectively. All the RNA-sequencing procedures were performed by Novogene Bioinformatics Technology Co., Ltd (Beijing, China). Sequencing libraries were prepared using a Poly-A tail capture method and sequenced with NovaSeq 6000.