GSM5550434: LG1 BMDM; Mus musculus; RNA-Seq
Bone marrow cells were flushed from tibias and femurs with cold Dulbecco's modified Eagle's medium (DMEM) and were cultured in DMEM supplemented with 10% fetal bovine serum (FBS), 1% penicillin/streptomycin, and 10 ng/ml macrophage colony-stimulating factor (M-CSF, PeproTech) for 6 days to generate BMDMs. Then treated with LPS andGramicidin or KCl for 6 hours. And total RNA were extracted for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols