GSM5395122: RNA_seq_Tektin-C_KD_rep2; Drosophila melanogaster; RNA-Seq
100 pairs of testes from < 2 day old male flies were used per replicate. The testis were dissected in PBS on ice. Total RNA was extracted using RNeasy Mini Kit (Qiagen 74104). Ribosomal RNA was depleted using Ribo-Zero rRNA Removal Kit (Illumina MRZH116). Library preparation was then carried out using SMARTer Stranded RNA-Seq Kit (Clonetech 634839).