GSM5643400: GEX_SFC856_Pool2; Homo sapiens; RNA-Seq
Then 1ug of cell hashing antibodies was added to each of the samples. Each cell line had 8 IBIDI dishes corresponding to the 8 different treatment conditions and 8 hashing antibodies. After 30 min incubation at 4o C, cells were washed 2 times: first wash by spinning them at 600g for 5min and resuspending them in 500ml staining buffer spinning, second wash by spinning the cells at 600g 5min and resuspending them in 200ul staining buffer. Finally, cells were resuspended in 150ul PBS, filtered through a 40um cell strainer and counted. All the treatments from a cell line were pooled together and were loaded on a 10X Chromium.  For SFC841-03-01, SFC856-03-04 and SFC180-01-01 cell line 10.000 cells per pool were loaded in one 10X Chromium lane, for SFC854-03-02 cell line 5000 cells per pool were loaded on two 10X Chromium lanes.