metagenome sequencing of murine fecal samples S2B
The samples were sent to AGRF for DNA extraction and amplicon sequencing. DNeasy PowerSoil Pro Kit (QIAGEN) was used with up to 50 mg of sample for the DNA extraction. The 16S V1-V3 region was PCR-amplified using the 27F (AGAGTTTGATCMTGGCTCAG ) and 519R (GWATTACCGCGGCKGCTG) primer set. The samples were pooled and sequenced on the Illumina MiSeq platform utilising illuminas XT Indexes and Paired End Sequencing chemistry.