GSM5777556: MHC-P-701_Well_H09; Mus musculus; RNA-Seq
The cells are collected in WTA single cell encoding plate, RNA secondary structure is done at 65C for 3 minutes (hold at 4C), mastermix containing BD customised RT enzymes is added (PN 650000041) and run at 42C for 30 minutes, heat inactivated at 80C for 5 minutes before holding at 4C. The samples is pooled and purify before library prep. Libraries of the cellular RNA were made using the BD WTA single cell kit and sequenced using MiSeq V2 from Illumina (BM-DC, BM-M, DN).