GSM6166325: RNA-seq suvhsuvh9_sdlg_2; Arabidopsis thaliana; RNA-Seq
For meiocyte, 1 U/μL Recombinant RNase Inhibitor (Takara, 2313A) was added to the collection buffer. 300-400 meiocyte masses were used to extract ~400-1000 ng RNA for a biological replicate. The RNA was extracted using Trizol (ThermoFisher, 15596026). For seedling, 4-5 plants were ground in liquid nitrogen and the total RNA was also extracted using Trizol. RNA-seq libraries were constructed with the TruSeq RNA Library Prep Kit v2 (Illumina) following the manufacturer's instructions