GSM6031531: Organoid-804B-Ctrl-D200-2; Homo sapiens; RNA-Seq
RNA was isolated from 3 frozen organoids per sample using the QIAGEN RNeasy Kit according to manufacturer's manual (n=3 individual organoids per sample). RNA quality was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies). RNA samples with RIN more than 7 were used for library generation using the TruSeq Library Preparation Kit (Illumina Inc.) following manufacturer's instructions.