GSM5229288: WT rep 2; Homo sapiens; ChIP-Seq
Cells were crosslinked and chromatin was isolated and sheared. Pol II-DNA bound complexes were purified by antibody precipitation. Libraries for Illumina sequencing were prepared from the Pol II ChIP eluates by the University of Colorado Anschutz Genomics and Microarray Core facility using the Tecan Ovation Ultralow DNA Library System. Sequencing was performed using an Illumina NovaSEQ6000, obtaining 2 × 150 bp reads.