GSM4777849: Time course 3, time point 28; Mus musculus; RNA-Seq
At specified time point, RNA collected with Qiagen RNeasy kit's Buffer RLT, homogenized via QIAshredder and then flash frozen. After all samples collected, RNeasy column extraction protocol continued according to Qiagen's instructions for animal tissue. RNA libraries created by Genewiz using rRNA depletion step and standard Illumina protocols for Paired End sequencing with Illumina HiSeq