RNA-seq of Ba/F3 cells pHR-SIN-CASZ1b-mRFP rep 1 - 6h starvation
Ba/F3-empty and Ba/F3-CASZ1b cells were grown in suspension in RPMI-10 (10% FBS) medium. The RNA was extracted from cells cultured for 6h in RPMI-10 (without the addition of IL3) using the RNeasy Mini Kit according to the manufacturers instructions (Qiagen). Sequencing was performed using a non-stranded mRNA library preparation generating paired-end 100-bp sequence tags using a BGISEQ-500 sequencer (BGI).