Shotgun sequencing of water sediment sample from a Patagonian fjord (marine sediment metagenome): 15,9 meters deep
We filtered the 15,9-meter deep marine sediment sample from Comau fjord through a 0.22 micron Sterivex to capture bacterial cells. Then, we extracted DNA from the 0.22 um filter through phenol-chloroform DNA extraction methodology, using Xanthogenate buffer for lysis step. Sequencing library were prepared using Illumina TruSeq DNA PCR-Free kit, following its sample preparation guide.