Baylor Hopkins Center for Mendelian Genetics (BH CMG) sample 107975-0196297240
A 50ng input of gDNA was sheared to 300bp using Covaris E220; libraries were generated using Kapa Hyper Prep Library prep reagents with custom indexed adapters synthesized from IDT (Integrated DNA Technologies); whole exome enrichment using Agilent SureSelect Human All Exon capture process and reagents with custom blockers and primers synthesized from IDT. The capture reagent used was Agilent SureSelect HumanAllExonV4_51MbKit_S03723314 or Agilent SureSelect HumanAllExonV5Clinical_S06588914.: LibraryPrep_LowInput_KHP_CIDR_Protocol_v1