RNA sequencing of MALAT1+/+ HeLa cell line, MALAT1-/- HeLa cell line, FL MALAT1 rescue in  MALAT1-/- HeLa cell line and mutant MALAT1 (Q123m) rescue in MALAT1-/- HeLa cell line
RNA isolation was performed from two independent biological replicates of HeLa cells for following 4 conditions- MALAT1+/+, MALAT1-/-, MALAT1-/- transfected for 48 hours with full length (FL) MALAT1 and MALAT1-/- transfected for 48 hours with mutant MALAT1 (Q123m). RNA sequencing libraries were prepared using Invitrogen Collibri Stranded RNA Library Prep Kit for Illumina with Collibri H/M/R rRNA Depletion kit as per the manufacturer's protocol. The resulting cDNA libraries were sequenced with 2 x 150 bp paired-end sequencing.