Bacterial Metatranscriptomics in human gut
Libraries were then constructed utilizing 5-10 ng RNA for each sample and using the Next Ultra II Directional RNA Library Prep Kit with rRNA depletion (New England Bioscience) in a paired end fashion with 2x150 base pair paired end reads. Libraries underwent quality control via tape station prior to multiplexing at a concentration of 4 nM, and sequencing was performed on an Illumina MiSeq platform at the University of Colorado Genomics core with >6 Gb data output per sample.