GSM5005289: mixed sample (non-demultiplexed); Mus musculus; RNA-Seq
24 hours post infection spleens were removed squashed against 70 um strainer followed by red blood lysis and stained with antibodies.  Single cells were sorted to 384 well plate containing lysis solution (UPW, 8nM poly-dT barcodes, 0.1%triton and RNAse inhibitors.) RNA-seq libraries were prepared according to MARS-seq libraries protocol (Jaitin et al Science 2014)