GSM5201880: E12.5_cKO3; Mus musculus; RNA-Seq
Forebrains were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Use Agilent 2100 Bioanalyzer (Agilent RNA 6000 Nano Kit) to detect the concentration of total RNA, RIN value, 28S/18S and fragment size. RNA libraries were prepared for sequencing using standard BGISEQ-500 protocols.