GSM5460998: TAK7_1; Marchantia polymorpha subsp. ruderalis; RNA-Seq
Gemmae were  flash frozen in liquid nitrogen. RNA was extracted using RNeasy Plant Mini Kit (Qiagen) with on-column DNase treatment. Individual cDNA libraries for sequencing were prepared from 100 ng total RNA using the TruSeq stranded mRNA library preparation kit including polyA selection (Cat# 20020594/5, Illumina Inc. The library preparation was accomplished according Illumina's protocol (#1000000040498)