GSM5351109: RPI11 A188xB73 Seedling RNA-seq; Zea mays; RNA-Seq
Whole V3 maize seedlings (roots and shoots) were removed from the ground, rinsed to remove dirt, and then frozen. Seedlings were ground to a powder with a mortal and pestil after freezing with liquid N2. Total RNA was then isolated using the Qiagen RNeasy Plant mini kit. Library protocol based on CelSeq2 (Hashimshony, et al. 2016). Read 1 contained a 10 bp unique molecular identifier (UMI) followed by a 6 bp sample barcode. Read 2 contained transcript sequence. Deposited files are read 2 with the UMI appended to the fastq entry headers, all samples have been demultiplexed using the 6 bp sample barcodes.