GSM5350797: IL17ra-/- 5; Mus musculus; RNA-Seq
Muscles from Il17ra-/- or wild type mice treated with VML and PCL were collected 6 week after surgery, submerged in Trizol reagent, and minced and grinded in Trizol. Qiagen RNeasy Mini Kits were used to purify RNA following TRIzol RNA extraction. Purified RNA was quantified using an Agilent 2100 Bioanalyzer RNA 6000 Nano Kit according to manufacturer's protocol. All RNA samples had RNA integrity score (RIN score) greater than 8.0. Libraries were then prepared with the Illumina TruSeq RNA Library Prep Kit V2 and sequencing at a depth of 30M unique reads per sample using an Illumina NovaSeq S2. TruSeq v2