GSM4728426: prostate_MSKCC-EF1_RNA-se; Homo sapiens; RNA-Seq
A fragment of frozen tissues (PDX models) or 50 um sections (liver metastases) were cut and homogenized in 1 ml of AllPrep DNA/RNA Mini Kit (Qiagen) using a plastic pestle (Cole-Palmer #44468-23). DNA and RNA were simultaneously isolated. 500 ng RNA was used to prepare libraries using the NEBNext Ultra™ RNA Library Prep Kit for Illumina. RNA quantity and quality were assessed on an Agilent 2100 Bioanalyzer. For all RNA-seq, reads were sequenced on a NextSeq 500 instrument (Illumina).