Intestinal microbiota development in relation to nutritional status in preterm infants
Total DNA from stool samples of infants born preterm. DNA was extracted using the Power Fecal DNA Isolation Kit (QIAGEN) with the bead-beating method. The V3-V4 region of the 16S rRNA gene was amplified with dual-indexed primers and sequenced at the Roy J. Carver Biotechnology Center at the University of Illinois on the Illumina MiSeq 2x250bp platform.