GSM5350050: BEP2DA [Hi-C]; Homo sapiens; Hi-C
Nuclei were extracted and the chromatin was applied with 4 cutter restriction enzyme (400 units MboI). The next steps included making the DNA ends with biotin-14-dCTP and performing blunt-end ligation of crosslinked fragments. Finally, after shearing to approximately 200-600 bp by sonication, the libraries were constructed through end-repair, A-tailing, adapter ligation and amplifying.