GSM5229301: scRNAseq_tumor_Ahr_cKO_rep1; Mus musculus; RNA-Seq
Tumors from three Ahrfl/fl or three LysM-CRE cKO mice were digested, pooled, and stained with DAPI. Live cells were FACS-sorted into buffer (PBS + 2% FBS). Cells were washed 2 times with PBS + 0.04% BSA, and then only 10x10^3 cells mixed with 10X Genomics Chromium single-cell RNA master mix, followed by loading onto a 10X Chromium chip according to the manufacturer's protocol to obtain single-cell cDNA.