GSM5821444: OIL 2; Mus musculus; RNA-Seq
The core facility for Bioinformatics and Expression Analysis at Karolinska Institutet performed the lysis of tumor tissue, RNA extraction and sequencing. Qiagen RNeasy mini kit (Qiagen) was used for RNA extraction from the tumor lysates. Quality control of total RNA was performed with Agilent Tapestation. Library constructs was prepared according to the Illumina TruSeq Stranded mRNA sample preparation protocol. Single-end reads of 75 bp generated using Illumina Nextseq 550. For each sample approximately 11,8 million reads were obtained.