GSM5821052: WGBS_ALL-SIL; Homo sapiens; Bisulfite-Seq
Cells at exponential growth phase were spun down at 1500 rpm for 3 minutes and cell pellet was used to extract genomic DNA. Whole genome bisulfite sequencing was performed on bisulfite-modified DNA-sequencing library generated according to Illumina's instructions accompanying the TruSeq DNA Methylation Kit (Part# EGMK81312). 200ng of genomic DNA, including 0.2% Lambda DNA (N6-methyladenine-free; NEB) was bisulfite converted in a single reaction using the EZ DNA Methylation Gold Kit (Zymo Research) as outlined by the manufacturer. The bisulfite converted DNA (bsDNA) was split into four aliquots per sample. Four single indexed WGBS libraries were constructed with the EpiGnome Methyl-Seq kit (EGMK81312, Epicentre) according to the manufacturer's recommendations. The final concentration of each library was accurately determined through qPCR (KAPA Biosystems). The four independent libraries were normalized, pooled, and loaded across three lanes of the HiSeq 2000 instrument (Illumina).