NGS of S. oneidensis:PAM1
The NNN-PAM pooled target plasmids (pTarget, 64 in total) were transformed into MR-1 harboring pCYR104 (dCas9-AID) . We picked all colonies on three LB plates to ensure the coverage of pool (coverage10, colonies 640) and induced for 24 h. Bacteria plasmids were extracted and the target range was amplified and then NGS sequenced.