GSM6329488: LSECs isolated from bevacizumab-sensitive HCT116 CRCLM xenografts #1 [1731-LSEC-C1]; Mus musculus; RNA-Seq
Total RNA was extracted using TRIzol Reagent (Servicebio, Wuhan, China) at a ratio of 106 cells per mL of reagent according to the manufacturer's instructions. RNA-sequencing libraries were generated using ribosomal RNA depleted RNAs with the NEBNext Ultra II RNA Library Prep Kit (NEB) according to the manufacturer's instructions. Libraries were controlled for quality and quantified using the Bioanalyzer 2100 system (Agilent Technologies, CA).