GSM5856728: ATACseq.E0771GFP-EV_2; Mus musculus; ATAC-seq
Transposition was performed using the Nextera DNA Library Prep Kit (Illumina, #20034197) with nuclei from 50,000 cells. DNA was purified with the DNA Clean & ConcentratorTM-5 kit (Zymoresearch, #D4013) and the libraries were amplified by PCR using the NEBNext HiFi 2x PCR Master Mix (NEB, #M0541S). PCR fragments were purified with Qiagen MinElute PCR Purification Kit and library concentration and quality were determined with the Agilent High Sensitive DNA Kit and Bioanalyzer, respectively. ATAC-seq libraries were sequenced with an Illumina HiSeq Platform with 100-bp paired-end reads in triplicates.