GSM5821289: KO3; Mus musculus; RNA-Seq
Liver tissue samples were homogenized, and total RNA was extracted using the Qiagen miRNeasy mini kit. cDNA libraries were constructed using an Illumina TruSeq RNA LS Sample Preparation kit v2 (Illumina, San Diego, CA, USA). Final individual cDNA libraries were assessed for fragment size and quality with the 2100 Bioanalyzer (Agilent, Santa Clara, CA, USA) and sequencing libraries were quantified with the ABI StepOnePlus Real-Time PCR System