GSM5351399: Allergen-treated mutant rep 1; Mus musculus; RNA-Seq
total RNA was extracted using TRIzol Reagent mRNA was enriched using oligo dT beads and then fragmented chemically by incubating at 94℃ for 8 min. cDNA was synthesized with SuperScript II. After purification using AMPure XP beads, the double-stranded cDNA was ligated to TruSeq RNA adapters followed by 15 cycles of amplification and library purification