NextSeq 500 paired end sequencing; RNA-seq of biopsies from the bowels of patients with Ulcerative Colitis classified as healed versus non-healed according to different classification scores
RNA-seq of biopsies from the bowels of patients with Ulcerative Colitis classified as healed versus non-healed according to different classification scores
Biopsy was put immediately into RNA later following collection and stored initially at 4C followed by -80C for longerterm storage prior to extraction RNA was extracted using the RNeasy Mini PLUS kit (Qiagen). Tissue was initially homogenised in RLT buffer by GentleMACs setting RNA-01 0.5ng total RNA was used to prepare uniquely indexed cDNA libraries using QIAseq UPX 3â€™ Transcriptome reagents (Qiagen)