Pacbio sequencing of C. botulinum Grp III
genomic DNA was fragmented (20 kbp) using Megaruptor (Diagenode). Blunt end hairpin adapters were ligated to DNA fragments after DNA damages and DNA ends were repaired, to prepare SMRTbell libraries. Sequencing primers and polymerase were bind to SMRTbell templates using PacBio DNA/Polymerase Binding Kit P5. SMRTcells were loaded with SMRTbell libraries using MagBead followed by 180 minutes sequencing using PacBio RSII instrument