GSM5395060: AV_015_F_2; Drosophila melanogaster; RNA-Seq
Flies were frozen and heads collected on dry ice. RNA extraction was done using the Direct-Zol microprep kit (ZymoResearch) We used the NuQuant+UDI, Drosophila AnyDeplete kit (Tecan) to deplete ribosomal RNA and prepare bar-coded cDNA libraries for sequencing after 17 cycles of amplification. Pooled 3nM libraries were run on a Novaseq6000 (Illumina).