GSM4820457: PS16.02.06; Homo sapiens; miRNA-Seq
Mature sperm were enriched by centrifugation through a 50% PureSperm density gradient (Nidacon). Sperm were lysed and homogenized in TRIzol-LS, supplemented with 0.2 M β-mercaptoethanol. Total RNAwas were isolated using Qiagen's miRNeasy Mini kit. small RNA libraries were constructed with 10 ng of small RNA using the TruSeq small RNA Library Prep Kit (Illumina) according to manufacturer's protocol.