GSM5582434: ciji_2: Osteoclast precursor cells 1 treated with DKK1; Mus musculus; RNA-Seq
Total RNA was harvested using Trizol reagent, and Nanodrop 2000 was used to detect the concentration and purity of the extracted RNA, agarose gel electrophoresis to detect RNA integrity, and Agilent 2100 to determine the RIN value. A single library building requires that the total amount of RNA is not less than 5μg, the concentration is ≥ 200ng/μL, and the OD260/280 is between 1.8~2.2 RNA libraries were prepared for sequencing using standard Illumina protocols