GSM5417601: control; Homo sapiens; RNA-Seq
Total RNA of each sample was extracted using RNeasy Mini Kit(Qiagen). Total RNA of each sample was quantified and qualified by Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA), NanoDrop (Thermo Fisher Scientific Inc.) and 1% agrose gel. 1 μg total RNA with RIN value above 6.5 was used for following library preparation. RNA libraries were prepared for sequencing using standard Illumina protocols