GSM6165959: HAP1_WT_ChIP-seq_AGO2; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, the cross-linked chromatin was sheared to the size ranging from 200 to 500 bp using a Sonic dismembrator (Thermo Fisher Scientific). The purified DNA fragments were subjected to Illumina sequencing library construction using NEBNext DNA Library Prep modules (New England Biolabs) and NEBNext Multiplex Oligos (New England Biolabs) according to the manufacturer's protocol.