Single cell RNA sequencing of mus musculus: adult male brain
The Single Cell 3 Protocol produces Illumina-ready sequencing libraries. A Single Cell 3 Library comprises standard Illumina paired-end constructs which begin and end with P5 and P7. The Single Cell 3 16 bp 10x Barcode and 10 bp UMI are encoded in Read 1, while Read 2 is used to sequence the cDNA fragment. Sample index sequences are incorporated as the i7 index read. Read 1 and Read 2 are standard Illumina sequencing primer sites used in paired-end sequencing.