GSM5645905: G1219-A5; Homo sapiens; RNA-Seq
Core needle of liver biopsies were stored in tissue storage solution (Miltenyi, 130-100-008) and transferred for dissociation within minimum ischemic time. On arrival, each sample was washed in PBS with 0.04% BSA (Beyotime, ST023), and then transferred to a gentleMACS C Tube (Miltenyi #130-093-237) containing 2.5 ml digestion enzyme mix prepared according to the kit user guide (Liver dissociation Kit from Miltenyi Biotech # 130-105-807). The cDNA libraries were prepared as recommended by the Single Cell 3' Reagent Kits v2 user guide. single cell RNA seq