GSM5416622: LV.RNA.Prdm16cKO1; Mus musculus; RNA-Seq
E13.5 embryonic hearts or isolated ventricles were homogenized in TRIzol (Invitrogen) and total RNA was isolated according to the manufacturer's instructions. The concentration and quality of purified RNA was assessed by TapeStation (Agilent). RNA integrity numbers (RIN) were in the range of 6.1 to 10. Libraries were sequenced with an Illumina HiSeq 4000 sequencer to a sequeencing depth of 20-70 million reads per sample. cDNA libraries were prepared using an Illumina TruSeq stranded mRNA kit according to manufacturer's instructions.