Illumina HiSeq 2000 sequencing; Analysis of microRNA expression in ADPKD patient derived urinary exosomes by RNAseq
Analysis of microRNA expression in ADPKD patient derived urinary exosomes by RNAseq
Samples were collected from urinary exosomes Urinary exosomal associated microRNAs were isolated from the exosome pellet using a miRCURY™ RNA cell and plant isolation kit (Exiqon) according to the manufacturers protocol. The Illumina® TruSeq Small RNA Sample Preparation Protocol was used to generate the small RNA libraries.