GSM5516196: KEN_D3_GOKM_rep2 [ATAC-Seq]; Homo sapiens; ATAC-seq
50,000 cells per replicate (two biological replicates per line) were incubated with 0.1% NP-40 to isolate nuclei. ATAC-Seq library preparation was performed as previously described (Buenrostro et al., 2013). After nuclei isolation, nuclei were then transposed for 30 min at 37C with adaptor-loaded Nextera Tn5 (Illumina, Fc-121-1030). Transposed fragments were directly PCR amplified and Sequenced on an Illumina NextSeq 500 platform to generate 2 x 36-bp paired-end reads.