GSM5229048: 5HT_1 [ATAC-seq]; Mus musculus; ATAC-seq
Cells were washed with PBS and incubated with lysis buffer (10mM Tris-HCl, 10mM NaCl, 3mM MgCl2, 0.1% IGEPAL) to purify nuclei. Illumina sequencing libraries were constructed by treating ~50,000 nuclei with Tn5 Transposase in TD buffer (Illumina) followed by amplification and purification with Qiagen MinElute PCR Purification Kit.