GSM5776782: D1_Boiled (Bacterial strains); Homo sapiens; RNA-Seq
Tubes were spun for 10 min at 450 × g without break. The pellets were resuspended in 2 ml of PAXgene reagent. Tubes were incubated at room temperature for 2 h and placed at 4°C overnight. RNA was then isolated using the PAXGene protocol. Libraries were generated for each donor using the CelSeq2 protocol (Hashimshony et al. Genome Biol 2016) and were sequenced on  Illumina NovaSeq.