TRIKAFTA metagenomics
The V4 hypervariable region of the 16S rRNA gene was amplified using Illumina compatible, dual indexed primers 515f/806 following the protocol developed in the Schloss lab (Kozich, JJ 2013). PCR products were batch normalized using an Invitrogen SequalPrep DNA Normalization plate and product recovered from the plates pooled. The pool was cleand up and concentrated using AMPure XP magnetic beads. The pool was QCd and quantified using a combination of Qubit dsDNA HS, Agilent 4200 TapeStation HS DNA1000 and Kapa Illumina Library Quantification qPCR assays.