Illumina HiSeq 4000 paired end sequencing; Single-cell RNA-seq of Endometrial Superficial biopsies and full depth uterine tissue from organ donors
Single-cell RNA-seq of Endometrial Superficial biopsies and full depth uterine tissue from organ donors
Full thickness uterine wall samples were obtained from deceased transplant organ donors (A13, A30) after ethical approval (reference 15/EE/0152, East of Englandâ€”Cambridge South Research Ethics Committee) and informed consent from the donor families. Uterus was removed within 1 hour of circulatory arrest. RNA was converted into cDNA and amplified as part of the 10x v2 chemistry Libraries were constructed using the 10x v2 chemistry