GSM5822620: pre-B cells RAG-/- µ+ E34.KO_2; Mus musculus; RNA-Seq
RNA was isolated from 1x10^5-2x10^6 cells using QIAshredder (Cat. #79654, Qiagen) and the RNeasyTM Plus Mini Kit (Cat. #74134, Qiagen) RNA sequencing libraries were generated using the Illumina Ribo-Zero Plus rRNA Depletion Kit with IDT for Illumina RNA UD Indexes (Illumina, San Diego, CA). Samples were processed following manufacturer's instructions. Resulting libraries were multiplexed and sequenced with 100 bp Paired End (PE100) to a depth of approximately 25 million reads per sample on an Illumina NovaSeq 6000.