RNA-seq of 200 mM NaCl treated Triticum aestivum (biological replicate 3)
o investigate the expression patterns of GST genes in wheat under salt stress, a common wheat cultivar (Chinese Spring) was planted in a growth chamber under a photoperiod of 16 h/8 h (light/dark). The seedlings were subjected to salt treatment at concentrations of 0, 100, 200, and 300 mM NaCl at the one week stage. The leaf tissues were harvested after one week of treatment and total RNA of all the collected samples was extracted using an RNAprep Pure Plant Kit (TIANGEN, Beijing, China). A NanoDrop 1000 spectrophotometer was used to determine the quantity and quality of the RNA. A total of 12 wheat samples (three biological replicates were conducted for each treatment) were sequenced at Novogene Co. Ltd (Beijing, China) and paired-end sequencing was performed with an Illumina HiSeq 2500 platform (Illumina, USA).