GSM4728363: 1572874946_3976_P; Homo sapiens; RNA-Seq
Dataset consists of a total of four single-cell datasets generated using    the 10x Genomics Chromium platform. Prior to GEM generation, tissue sample    was taken fresh from OR rinses 3x in phosphate buffered saline, cut into    1-3mm sized pieces and gently digested in Liberase TL solution for 1-hr at    37C. The reaction was quenched with serum and digested cell suspension were    filtered through a 70micron filter centrifuged  gently at 4C and rinsed x2    with pbs followed by lysis of red blood cells with an rbc lysis solution.    This was followed by 2 rinses with phosphate buffered saline. Viability was    assessed by trypan blue of a small aliquot and at this point cells were    used for the Next-generation sequencing libraries that were prepared    following the 10X Single Cell v2 protocol without modification. Sample    quality was evaluated using Agilentâ€™s Bioanalyzer High Sensitivity reagent    kit following cDNA amplification and library construction. The completed    libraries were quantified using the Kapa Library Quantification Kit and the    Life Technologies Viia7 Real-time PCR instrument. Libraries were sequenced    in a paired-end, single-indexed format on the Illumina. Cells were    sequenced to target a depth of at least 27,000 reads per cell