GSM5583461: DMSO - m6A_rep3; Rattus norvegicus; RIP-Seq
The whole RNA of NRCMs treated with CYP or DMSO was harvested using Trizol reagent.Total RNA of the sample was subjected to test the RNA integrity using agarose gel electrophoresis, then quantified and examined by NanoDrop ND-1000.1-2 ug of total RNA was selected for each sample for sequencing library construction. After polyA mRNA was enriched by oligo dT method, mRNA was interrupted into 100-300nt fragment, and using m6A antibody for enriching methylated RNAs Libraries were prepared according to Illumina's instructions accompanying the DNA Sample(KitKAPA Stranded mRNA-seq Kit). Including cDNA synthesis, add A in 3'end, 3'adaptor ligation, PCR amplication, purify cDNA library.