GSM5416646: Pythium oligandrum self-confrontation replicate 3; Pythium oligandrum; RNA-Seq
The sample was mixed with 1 mL of Trizol before the addition of 200 µl chloroform: isoamyl alcohol (24:1). The RNA was precipitated by mixing 0.8 volume of isopropanol with the aqueous phase from the previous step and the resulting pellet was washed once with 70% EtOH before resuspension in DEPC-treated water. The RNA was then cleaned-up using a Tiangen Plant RNA extraction kit which included an on-column DNase treatment. RNA integrity was visualised using an agarose gel and a Bioanalyzer, and RNA quantity and purity using a nanodrop spectrophotometer. A paired library 2x150 bp was prepared using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina).