GSM5460867: CCND1C1; Mus musculus; RNA-Seq
The tumor tissues were washed by phosphate buffer saline. The tissues were used in following study, RNA was harvested using Trizol reagent. RNA quality and quantity were measured by using Nanodrop spectrophotometer (ND-1000, Nanodrop Technologies) and RNA Integrity was determined by gel electrophoresis. Libraries were prepared by using KAPA Stranded RNA-Seq Library Prep Kit (Illumina). Briefly, total RNA of each sample was used to prepare the RNA sequencing library 1) RNA fragment; 2) Random primed first strand cDNA synthesis; 3) dUTP based second strand cDNA synthesis; 4) Adaptor ligation and PCR amplification.