GSM5417090: PAC2NKAE; Homo sapiens; RNA-Seq
Cell pellets were frozen in RLT buffer. RNA was extracted using the AllPrep DNA/RNA/protein Mini Kit (Qiagen) Ribosomal RNA was depleted with the NEBNext rRNA Depletion Kit (E6310L) and RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760S), using indexes from NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1, E7600S). Reads were sequenced in paired-end fashion (76bp x 2) in a NextSeq 500/550 sequencer, with a High Output v2 kit. Stranded RNA-seq