Chicago HiC Seq of Bicyclus anynana: wandering larvae
Eighteen female siblings were harvested at wandering stage. Guts were removed and the larvae were snap frozen immediately using liquid N2. And tissues were sent to Dovetail Genomics, USA for Chicago library preparation and sequencing. Chicago library preparation involves invitro chromatin fixation, digestion, and crosslinking of regions in the genome that are close to each other in the 3D chromatin structure to order and scaffold the genome