GSM6031434: skin tissue-SCC1; Homo sapiens; RNA-Seq
All the samples were incubated with dispase for 2 h at 37 °C. The epidermis was peeled from the dermis and minced with scissors to pieces < 1 mm3, incubation with 5mL of 0.25% trypsin-EDTA for 30 minutes. The dissociated cell suspension was washed with 10% PBS, filtered through a 70 mm filter, centrifuged at 1500 rpm for 5 min. Dermis were incubated with 5mL of 0.35% collagenase for 30 minutes. All steps other than incubation were performed on ice. Cells were loaded according to standard protocol of the Chromium single cell 3' kit and the v2 chemistry from 10X Genomics. The library was pooled and sequenced on the Illumina HiSeq 6000 platform to get ~150-200 million reads.