Illumina NovaSeq 6000 sequencing; RNA Sequencing of CD169+ lymph node macrophages isolated from naïve or tumor-bearing mice
RNA Sequencing of CD169+ lymph node macrophages isolated from naïve or tumor-bearing mice
Living CD3-CD19-Ly6G-CD11b+ F4/80- or F4/80+ CD169+ lymph node macrophages were sorted for RNA sequencing Cells were sorted directly into RNA lysis buffer and kept at -80 °C until RNA extraction Libraries were generated using the NuGEN Ovation Single Cell RNA-Seq System