Illumina HiSeq 1500 sequencing; Cytosine methylation of mature microRNAs inhibits their functions and is associated with poor prognosis in glioblastoma multiforme
Cytosine methylation of mature microRNAs inhibits their functions and is associated with poor prognosis in glioblastoma multiforme
5 millions of cultured U87 cells were used for one extraction. miRNA extractions were performed using the NucleoSpin® miRNA kit (Macherey Nagel , France) according to the manufacturer's instructions. Libraries were prepared from 30 ng of small RNAs with the NEBNext Small RNA Library kit (Biolabs, France)