RNA-Seq of Phyllotreta armoraciae: adult foregut
Total RNA was extracted from different tissues of newly emerged P. armoraciae beetles using the innuPREP RNA Mini Kit (Analytik Jena). Tissues from at least 10 beetles were pooled per sample. RNA integrity was verified using an Agilent Technologies 2100 Bioanalyzer with the RNA 6000 Nano Kit (Agilent Technologies). RNA quantity was determined using a Nanodrop ND-1000 spectrophotometer (PEQlab Biotechnologie GmbH).