GSM5196484: TFAP2A ChIP-seq replicate2; Homo sapiens; ChIP-Seq
0.005 µg of polyclonal ChIP Grade TFAP2A antibody (ab52222, abcam®, Great Britain) were added to 25 µg of DNA. The treated samples incubated with rotation at 4°C overnight. Protein G magnetic beads were used to  pull down the fragments and removed by incubation with ChIP elution buffer on a shaking thermomixer at 65°C for 30 min. To reverse DNA-crosslinking, the supernatant was treated with NaCl and Proteinase K overnight and purified using spin column tubes (provided in the kit). The NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs® GmbH, USA) was used according to the manufacturer's protocol. AmPure XP beads (Beckman Coulter™, USA) were used for clean up steps. No size selection was performed. Samples were equimolarly pooled. The library quality was controlled on an Agilent High Sensitivity D1000 system and samples were diluted to a final concentration of 2 nM. The replicates were sequenced twice on an Illumina MiSeq v2, yielding ~ 20 million 2x250bp paired end reads for each sample (ChIP and input control). Transcription factor ChIP-Seq