GSM4820445: YBX1-Flag-IP-rep2; Homo sapiens; RIP-Seq
RNA was harvested using Trizol reagent.mRNA are isolated，fragmented and Ybx1-specific antibodies are used to immunoprecipitate RNA. Ybx1 binding RNA is reverse-transcribed to cDNA through first strand synthesis and second strand synthesis Illumina TruSeq DNA Sample Prep Kit (Cat#15026486) was used with DNA for the construction of sequencing libraries. Libraries were prepared for sequencing using Illumina protocols.