non-targeting sira
Total RNA was isolated using the RNeasy RNA extraction kit (Qiagen) as per manufacturer guidelines.  RNA purity and concentration were measured on a NanoDrop (Thermo Fisher Scientific).  Nugen Universal Plus mRNA-seq Library Preperation (Tecan) was used for next generation library construction per the manufacturers protocols.  The directional mRNA template libraries were then sequenced as paired end 150bp reads on the Novaseq 6000 platform at the Univeristy of Colorado Genomics Core Facility.