GSM4728490: Suv39h1, Suv39h2 double knock-out, RNA-Seq replicate 2; Mus musculus; RNA-Seq
Single cell suspensions were generated from mouse thymus by mechanical homogenization following by red blood cell lysis (156mM NH4Cl, 11.9mM NaHCO3, 97μM EDTA). DP thymocyte cells were then flow-cytometrically sorted as CD45.2+CD19-CD4+CD8+ cells on a BD FACSAria™ cell sorter. RNA was extracted using a Qiagen Rneasy Plus Kit. Illumina TruSeq RNA Library Prep