GSM4728318: ESC_MEF- H1 iCas9 c4-T0; Homo sapiens; OTHER
Genomic DNA was extracted from screen cell pellets using the Wizard Genomic DNA Purification kit (Promega) according to manufacturer's instructions. Sequencing libraries were preparaed by amplifying gRNA inserts via a 2-step PCR using primers that include Illumina TruSeq adaptors with i5 and i7 indices.