GSM5822665: IL6_hep_rep2; Mus musculus; RNA-Seq
Liver was perfused using three-steps methods. Cells in liver were sorted by BD FACSAriaIII. RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols