GSM5643896: Gene expression Sample 1; Mus musculus; RNA-Seq
Beads with oligonucleotide barcodes were added to saturation to pair the beads with the cell in the microwells. Cell-lysis buffer was added to lysis the cell and allow barcode conjugated beads to capture poly-adenylated molecules, including mRNA, AbSeq targets and sample tag. Ab-Oligos/Sample Tag with cell barcode and UMI information were denatured off of the Cell Capture Beads and saved for AbSeq/Sample Tag amplification. For WTA library Preparation, random priming extension was performed on cDNA archived on Cell Capture Beads.