GSM4777942: 4_ChAT+; Mus musculus; RNA-Seq
Single cells were captured and lysed, and cDNA was synthesized and amplified in accordance to the 10X Genomics Single Cell 3' v3 Reagent Kit user guide. The amplified cDNA was used to construct Illumina sequencing libraries. Constructed libraries were validated and quantified with the High Sensitivity DNA chip and by real-time qPCR, and subsequently sequenced on an Illumina NextSeq 500 in high-output mode.