Adding value to cheese whey using it for hydrogen production in a thermophilic fluidized bed reactor by applying glycerol as a co-substrate
Genomic DNA from the samples was extracted using the FastDNA SPIN Kit for Soil DNA Extraction (MP Biomedicals) following the manufacturer's recommendations. The DNA extracted from the samples was quantified (ng/L), and the purity (ratio 260/280nm) was determined using Nanodrop 2000 Spectrophotometers (ThermoFisher Scientific). The integrity of the DNA was verified on a 0.8% agarose gel. The sequencing and bioinformatics services were performed by the company GenomeDX  Advanced Genetics (Rio de Janeiro, RJ). In the amplification of 16S rRNA Genes (Region V3+V4), Phusion High-Fidelity PCR Master Mix (New England Biolabs) and the primer set 341F - 806R (Yu et al., 2005) with barcode were used. Sequencing libraries were performed with NEBNext Ultra DNA Library Pre Kit for Illumina, as per manufacturer's recommendations.