DNA-Seq of Streptomyces griseorubiginosus DSM 40469
Genomic DNA was isolated using an extraction method optimized for Streptomyces species including three additional steps: mechanical treatment with glass beads, treatment with lysozyme solution (in TE buffer, pH 8.0) at 100 mg ml1 at 37C (16h), and achromopeptidase treatment (60 U) at 37C (30min) followed by further purification using the MasterPure Yeast DNA purification kit (Epicentre; Illumina Company). The sequencing library was prepared using the TruSeq DNA sequencing kit and was  sequenced on an Illumina MiSeq machine in a 2x 300 nt run.