Illumina HiSeq 1500 sequencing; RNA-seq of human RNA contaminated with different amounts of mouse gDNA to quantify the impact of gDNA contamination in prime-seq
RNA-seq of human RNA contaminated with different amounts of mouse gDNA to quantify the impact of gDNA contamination in prime-seq
Frozen vials of mouse embryonic stem cells (mESC) in Bambanker, and HEK293T cells were thawed and put into the corresponding lysis buffer DNA was extracted from 1 million mESCs using DNeasy Blood & Tissue Kit and RNA was extracted from 450,000 HEK293T cells using the Direct-zol RNA MicroPrep Kit  The prime-seq protocol was performed as described here: https://www.protocols.io/view/prime-seq-s9veh66