GSM5515756: Control knockdown-1; Homo sapiens; RNA-Seq
RNA was isolated by TRIzol-LS following manufacturer's protocol (ThermoFisher Scientific), and then further purified using RNeasy Micro columns (Qiagen) and quantified by QuBit (ABI). Quality of total RNA was assessed with BioAnalyzer Nano (Agilent) and all samples had a RIN above 8. 250 ng of total RNA was used for library preparation. Libraries were constructed using the KAPA RNA PolyA Stranded kit