GSM6052337: H3K9me3_Smchd1WT_MEF; Mus musculus; ChIP-Seq
ChIP was performed as described by Nozawa et al., (2013) Libraries were generated using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) according to the manufacturer's instructions. Size fractionation was carried out to enrich fragments of 100-300 bp from purified DNA for library preparation as well as the library around the size of 200 bp fragments after PCR amplification using AMPure XP (Beckman Coulter) magnetic beads.