RNA sequence of HepG2 trasfected with  shTRMT5_2
mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. Fragmentation was carried out and then first strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase(RNase H-). Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H.