GSM5350205: JhS2Arep2; Saccharomyces cerevisiae; RNA-Seq
Lysates were clarified from sonicated yeast cell pellets and histone-DNA complexes were isolated with antibody against  H3 (ab1791, Abcam),H3K4me3 (Abclonal; A2357),FLAG (F1804-1MG; Sigma-Aldrich),Rpd3 (sc-514160; Santa Cruz Biotechnology) Libraries were prepared using the HTP KAPA kit