GSM5351253: GluN3A Bic1h rep1; Mus musculus; RNA-Seq
Total RNA from cultured rat cortical neurons was isolated using the Nucleospin RNA (Macherey-  Nagel). RNA concentration and purity were assessed with NanoDrop™. RNA quality was  determined by the RNA Integrity Number (RIN) algorithm using the Agilent® 2100 Bionalyzer  Instrument; only samples with RIN>9 matched our standard. For RNAseq experiments, we performed bulk mRNA sequencing single-end with a length of  50bp using the RNAseq Illumina Hiseq2500.