GSM5395149: MeA5a_t20_E6; Homo sapiens; OTHER
Genomic DNA was extracted with a kit-free protocol based on cell lysis followed by ethanol extraction (see paper for a detailed version) For whole exome sequencing, Genomic DNA (gDNA) samples were quantified using the Promega QuantiFluor dsDNA system on the GloMax Multi Detection System following the manufacturer's guidelines. Samples were qualified using the Agilent Genomic DNA ScreenTape System run on the TapeStation 4200 according to the manufacturer's instructions. Subsequently, gDNA was fragmented using Covaris LE220-plus focused ultrasonicator and then prepared into libraries using the NEBNext Ultra II DNA library prep kit according to manufacturer's instructions.  Libraries were then combined in 8-plex and exonic regions enriched via hybridisation using the Twist Human Core Exon kit according to the manufacurer's instructions.  Libraries were sequenced on the HiSeq 4000 using paired end 100 bp reads.