GSM5461494: L_CON_S1; Bos taurus; Bisulfite-Seq
The DNA was extracted from the cell pellets with the DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany) and quantified using the PicoGreen DNA Quantification Kit (Invitrogen). Libraries were bisulfite converted and PCR amplified prior to sequencing with NovaSeq S Prime in the 150 paired-end reads mode (Illumina, San Diego, CA).