WGS of HEK293T treated with pooled serum from PDAC patients
Genomic DNA extracted using Exgene Clinic SV mini extraction kit (Cat. No. 108-101, GeneAll biotechnology Ltd, Korea). DNA was treated with RNase, eluted in 40l of deionized water and run on 1% agarose gel for integrity evaluation. The total sample quantity was determined on Qubit fluorometer (Cat. No. Q33226, Invitrogen). The WGS was carried out on the NovaSeqTM 6000 platform (Cat. No. 20012850, Illumina, USA) in a Pair-End 2 x 150 bp setup.