GSM5643900: Cortex_cytoplasm_control4 [Cx_cyt_S02-071]; Homo sapiens; RNA-Seq
Complete details can be found on manuscripts methods section. Briefly, subcellular fractionation was performed isolating pure cytosolic and nuclei cell compartments. Isolated cytoplasmic and nuclei samples were stored in Qiazol (Qiagen) at -80oC until RNA extraction was performed. Total RNA was extracted using miRNeasy kit (Qiagen), according to manufacturer's protocol. A total of 2 ug of RNA per sample in a 5 ul volume was rRNA-depleted using the Ribo-Zero Magnetic Kit (human/mouse/rat; Illumina) before library preparation. Stranded, paired-end sequencing libraries were prepared using the ScriptSeq v2 (Epicentre), after which samples were subjected to quality control on the 2100 Bioanalyzer (Agilent). Sequencing was performed as paired end 100 bp reads on an Illumina HiSeq 4000 sequencer at the Beijing Genomics Institute (BGI) (Hongkong, China).