RNA-Seq of Magnolia x soulangeana
Total RNA was extracted from the fresh plant tissues which were collected above using improved CTAB-PVP method, The mRNA fragments containing Poly(A) were enriched by Oligo (dT) magnetic beads, and then interrupted by fragmentation Buffer. Random N6 primer was added for reverse transcription, and further, second strand cDNA synthesis. After end repair and bubble adapter ligation, these double-stranded cDNA fragments were subjected to PCR amplification. Finally, six cDNA libraries were created from PCR products which were suffered from heat separation.