GSM5777219: CD4 Naïve T cells of wildtype control, replicate2 [WT_forKO_CD4_Na_rep2]; Mus musculus; OTHER
CD4 and CD8 Naive T cells were sorted from spleen using antibodies against CD8a, CD19, CD4, CD3e, CD62L, CD45R/B220, CD44, and CD16. Total RNA from sorted cells was isolated by using RNeasy plus micro (QIAGEN, 74034). 50 ng for each sample was used for the reverse transcription with barcoded primer BU3 followed by the purification, second strand synthesis and tagmentation following the original BRB-seq protocol (Alpern et al., 2019 Genome Biology) but using AMPure XP for purification.