GSM5351623: High Fat High Sugar Diet 4; Mesocricetus auratus; RNA-Seq
RNA was collected from each sample and passed through Qiashredder column (Qiagen) at 21,000 x g for 2 min to homogenize any remaining genomic DNA in the aqueous phase. Aqueous phase was combined with 600 µL of RLT lysis buffer (Qiagen, Valencia, CA) with 1% beta mercaptoethanol (MilliporeSigma) and RNA was extracted using Qiagen AllPrep DNA/RNA 96-well system. One hundred nanograms of RNA was used as input for rRNA depletion and NGS library preparation following the Illumina Stranded Total RNA Prep Ligation with Ribo-Zero Plus workflow (Illumina)