Genome sequencing of Bifidobacterium longum subsp. longum BCRC 12585
The genomic DNA of Bifidobacterium longum (subsp. Longum, BCRC 12585) was extracted from the enriched medium culture. Genomic DNA library constructed for Illumina sequencing was fragmentized to ~550bp by ultrasonicator and then conjugated the Truseq indexed adapters using TruSeq DNA PCR-free LT sample preparation kit (Illumina, USA). Sequencing was performed on an Illumina MiSeq using the V3 Reagent kit with 300bp paired-end configuration by Health GeneTech Corp. (Taoyuan, Taiwan).