GSM5778097: Elnf_P8_AscAo [ctrl]; Mus musculus; RNA-Seq
P8 ascending aortae were dissected out and pooled on iced PBS, digestd in an enzymatic mix (collagenase I [657 U/mL], collagenase XI [187.5 U/mL], hyaluronidase [90 U/mL], Dnase I [90U/mL] in HBSS) for ~20 min, filtered through a 70 um strainer, and resuspended in RPMI 1640 with 5% FBS. FACS was performed to isolate live singlets with DAPI as negative viability marker. The sorted cells were resuspended in PBS/0.04% BSA for scRNA-seq library preparation using 10x Chromium 3' v3 kit. 10x Chromium 3' v3