Short read sequences for Staph_12b
The shotgun genomic libraries were prepared with the Hyper Library construction kit from Kapa Biosystems (Roche). The library pool was quantitated by qPCR and sequenced on one MiSeq Nano flowcell for 251 cycles from each end of the fragments using a MiSeq 500-cycle sequencing kit version 2. Fastq files were generated and demultiplexed with the bcl2fastq v2.20 Conversion Software (Illumina). Phix DNA is used as a spike-in control for MiSeq runs. Most Phix reads are removed during processing but a few residual reads may remain. Phix genome is 3kb in length. Adaptors have been trimmed from the 3'-end of the reads. Sequence of adaptors used to make the libraries (used the first 33nt for adaptor trimming). Adaptor sequence in read1: AGATCGGAAGAGCACACGTCTGAACTCCAGTCACNNNNNNNNATCTCGTATGCCGTCTTCTGCTTG (NNNNNNNN= 8 nt index). Adaptor sequence in read2: AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTNNNNNNNNAGATCTCGGTGGTCGCCGTATCAT.