GSM5417004: Treatment 2 [S234_09B_CHG009177-hunyang1-C_L004]; Mus musculus; RNA-Seq
RNA was isolated using the standard TRIzol protocol. After the total RNA extraction and DNase I treatment, magnetic beads with Oligo (dT) were used to isolate mRNA, then fragmented into short fragments. cDNA was synthesized using the mRNA fragments as templates. Short fragments were purified and resolved with EB buffer for end reparation and single nucleotide A (adenine) addition. After that, the short fragments were connected with adapters. After agarose gel electrophoresis, the suitable fragments were selected for the PCR amplification as templates.