Optimizing DNA extraction methods for assessing organic enrichment in marine farm sediments
Polymerase chain reactions (PCR) were performed on all extracted samples (n=60) and targeted two genes. Eukaryotic communities were targeted using the primer set Uni18SF: 5- AGG GCA AKY CTG GTG CCA GC-3 and Uni18SR: 5-GRC GGT ATC TRA TCG YCT T-3 (Zhan et al. 2013), which amplified the 18S rRNA gene (V4 region).