GSM5004483: MK886-PMCs-3-A03_2018-7-11; Lytechinus variegatus; RNA-Seq
Individual PMCs were sorted directly into RNA lysis buffer supplemented with 1% β-mercaptoethanol. Transcripts were purified, reverse-transcribed, and amplified according to the SMART-Seq2 protocol (Tombetta et al., Curr Protoc Mol Biol, 2014). Libraries were prepared using the Nextera XT kit (Illumina) according to the manufacturer's instructions. Libraries were sequenced on an Illumina NextSeq 500 using 100bp paired end reads.