Bemisiatabaci:singlebacteriocytetranscriptome
Thesinglecellsampleswerecollectedintubeswithlysiscomponentandribonucleaseinhibitor.ThenwecarriedouttheamplificationbytheSmartSeq2method.ThenthecDNAproductionwascheckedbyQubit3.0FlurometerandAgilent2100Bioanalyzer.ThenthecDNAwasshearedrandomlybyultrasonicwavesforIlluminalibrarypreparationprotocolincludingDNAfragmentation,endrepair,3endsAtailing,adapterligation,PCRamplificationandlibraryvalidation.