GSM6032198: H460 cells,ISP-I (10μM) treatment for 48h,rep3; Homo sapiens; RNA-Seq
Total RNA extraction from non-treated as well as ISP-I treated NSCLC cells (3 replicates per group) was done by TRIzol reagent (Invitrogen, Carlsbad, CA, USA)and RNeasy Mini Kit (Qiagen, Valencia, CA, USA). The concentrations, quality as well as integrity of extracted RNA were assessed by NanoDrop spectrophotometry (Thermo Scientific). Sequencing libraries were constructed by TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA, USA)