Metatranscriptome from vaginal wash from Nellore Brazilian heifers
RNA was extracted using Maxwell viral total nucleic acid purification kit and cDNA was generated using Qiagen RT First Strand KIT. The second strand cDNA was synthesized by adding 20 l of Second Strand Marking Master Mix from Illumina Truseq Stranded mRNA library preparation kit and quantified using the Qubit Fluorometer. Library was prepped and sequenced by Omega Bio-tek, Norcross, GA.