Optimizing DNA extraction methods for assessing organic enrichment in marine farm sediments
Polymerase chain reactions (PCR) were performed on all extracted samples (n=60) and targeted two genes. Prokaryotic communities were amplified using a 16S rRNA gene (V3-V4 region) with the primer set 341F: 5-CCT ACG GGN GGC WGC AG-3 (Herlemann et al. 2011)