Illumina MiSeq paired end sequencing; 16S sequencing of fecal samples from adult willdtype and Foxc2lecKO mice at different timepoints of Foxc2 depletion and 16 sequencing of fecal samples from antibiotic treated adult willdtype and Foxc2lecKO mice.
bacterial 16S rRNA gene v1-v2 region (F27-R338)
The library was constructed following the basic protocol for intestinal content of mice described in Rapin et al. 2017. Microbiota Analysis Using an Illumina MiSeq Platform to Sequence 16S rRNA Genes. Curr. Protoc. Mouse Biol. 7, 100â€“129. https://doi.org/10.1002/cpmo.29.