WGS of E. coli UMB1335
DNA was extracted using the QIAmp DNA Mini kit with the following exceptions: samples were not vortexed, rather agitated by hand, and the lysis step was conducted for 1.5-2 hours at 37C. DNA libraries were prepared using the Rapid Barcoding 96 kit (Oxford Nanopore, SQK-RBK110.96), according to the manufacturers instructions, and sequenced on an Oxford Nanopore GridION using an R9.4.2 flow cell.