Donor11 Control 5h
An Illumina amplicon library was then performed following the 16S rRNA gene Metagenomic Sequencing Library Preparation Illumina protocol (Part #15044223 Rev. A), targeting the 16S rRNA gene V3 and V4 regions, resulting in a single amplicon of 460 bp. Amplicons were sequenced on a MiSeq Sequencer according to manufacturers instructions (Illumina, San Diego, California, USA) using the 2  300 bp paired-ends protocol.