GSM5460771: IL-1_1; Mus musculus; RNA-Seq
Mouse islets were lyzed in Trizol reagent and RNA was extracted using the RNeasy® Micro Kit (Qiagen). Single-end sequencing libraries were constructed from 250 ng of isolated RNA using the NEBNext® Ultra™ II RNA Library Prep Kit #E7770S (Illumina®). RNA sequencing was performed on a HiSeq 4000 System (Illumina®).