GSM5822675: H446 (circRNA-Seq); Homo sapiens; ncRNA-Seq
Total RNA was isolated from cells using the Magzol Reagent (Magen, China)according to the manufacturer's protocol Briefly, rRNAs were removed from Total RNA using RiboCop rRNA Depletion Trial kit(LEXOGEN，Australia) , then RNA was treated with RNase R (Epicentre, USA) and fragmented to approximately 200bp. Subsequently, the purified RNA fragments were subjected to first strand and second strand cDNA synthesis following by adaptor ligation and enrichment with a low-cycle according to instructions of NEBNext® Ultra™ RNA Library Prep Kit for Illumina (NEB, USA). Libraries were sequenced on the Illumina HiSeq xten with paired-end 150bp following the manufacturer's protocols.