GSM5598347: GBM cell line U251_Control; Homo sapiens; RNA-Seq
Cells were harvested and RNA was extracted by using Trizol reagent. In total, 3µg of RNA per sample was used as input material for the preparations. Sequencing libraries were generated using the NEB Next® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA, #E7530L) following the manufacturer's recommendations, and index codes were added to attribute sequences to each sample. The library preparations were sequenced on an Illumina platform, and 125bp/150bp paired-end reads were generated.