GBS of V. vinifera: population 50042 genotype 7209Z
The genotyping-by-sequencing data was generated following the Elshire et al (2011) method and included the following changes: 100ng of genomic DNA were used, 3.6 ng of total adapters were used, the genomic DNAs were restricted with ApeKI enzyme and the library was amplified with 18 PCR cycles.Elshire RJ, Glaubitz JC, Sun Q, Poland JA, Kawamoto K, Buckler ES, Mitchell SE. (2011) A robust, simple genotyping-by-sequencing (GBS) approach for high diversity species. PLoS ONE 6(5): e19379