GSM5292389: LV_8h rep2; Mus musculus; RNA-Seq
Heart is isolated from 12 weeks old mice. 10 mL HTK solution was infused for preservation. Isolated hearts are then stored in ice-cold HTK for 0, 4, and 8hs, then connect to ex vivo perfusion system. After 1h of perfusion in Krebs buffer, the LV and RV was harvested and snap-freezed in liquid nitrogen. Total RNA from LV and RV was then isolated using Trizol followed by on column DNAse I digestion using qiagen RNeasy Mini kit. 1 µg total RNA was used for RNA library construction using NEB ultraII directional kit.