GSM5856726: SC7; Mus musculus; RNA-Seq
all lived cells were used for sequencing Libraries were generated using Chromium Single Cell 3'Library & Gel Bead Kit v.2 (10x Genomics) according to the manufacturer's protocol. Briefly, cells were counted, diluted at 1000 cells/µL in PBS+0,04% FBS and 20 000 cells were loaded in the 10x Chromium Controller to generate single-cell gel-beads in emulsion. After reverse transcription, gel-beads in emulsion were disrupted. Barcoded complementary DNA was isolated and amplified by PCR. Following fragmentation, end repair and A-tailing, sample indexes were added during index PCR.