GSM4727521: zinc supplemented drinking water, GrWT, rep2 [WT_ZN1-2]; Mus musculus; RNA-Seq
ileum was isolated, washed with PBS and stored in RNAlater for RNA extraction, IEC samples for RNA-isolations were prepared as follow: +/- 5 cm of the distal ileum was dissected, flushed with ice-cold PBS and incubated with lysis buffer (732 6802, Bio-Rad) supplemented with 2-mercaptoethanol on ice for 5 min, after which the inner part of the bowel was scraped out and snap-frozen in liquid nitrogen. RNA extractions were done with RNeasy Mini Kit (Qiagen) Illumina truseq RNA stranded library protocol (for single end sequencing)