NextSeq 500 sequencing; ChIP-seq of MLL3 Delta mice liver and wild-type littermate controls
ChIP-seq of MLL3 Delta mice liver and wild-type littermate controls
Livers were harvested at ZT18 and immediately frozen before storage at -80. Samples were fixed in 4% formaldehyde for 10 minutes immediately upon thawing, before isolation of nuclei and immunoprecipitation specific histone-DNA complexes. Nucleic acid was extracted by phenol-chloroform and fragmented by sonication.  ChIP-seq libraries were generated from the ChIP- and Input-DNA using a custom Illumina library type on an  automated system (Apollo 342, Wafergen Biosystems/Takara).