Human RPE Cell free PolyA mRNA: Extracellular vesicles
cDNA was prepared from EV RNA using the Single Cell RNA kit SMART-Seq_V4 Ultra Low Input RNA Kit (634890, Takara, Mountain View, CA, USA) according to manufacturer instructions using 1ng input. Next, poly-A libraries were prepared using the SMARTer ThruPLEX DNA-seq Kit (R400675, Takara, Mountain View, CA, USA)