GSM4729082: N109L: Naïve Control rep3; Mus musculus; RNA-Seq
Left lungs were immersed in RNAlater™ Storage Solution at 4ºC for 24 h and then stored at -80ºC before delivery for RNA library preparation and high throughput RNA-sequencing  (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. Library preparation was conducted by Novogene Inc. (Sacramento, CA) using NEBNext Ultra II RNA Library Prep Kit for Illumina (New England BioLabs Inc., Ipswich, MA). Barcoded libraries were assessed using Qubit 2.0 (ThermoFisher, Invitrogen, Grand Island, NY), Agilent 2100 Bioanalzyer (Agilent Technologies), and quantified with qPCR. Individual libraries were pooled at equal molar concentrations and sequencing was performed using the Illumina NovaSeq 6000 platform producing paired-end 150 base pair reads and Q30 >80%.