GSM5395090: Sagittal plane of mouse embryo of E12.5; Mus musculus; RNA-Seq
Dissections were performed and embryos of E9.5-E12.5 were immediately frozen in liquid nitrogen-cooled isopentane and were embedded in pre-cooled OCT. Tissue preparation and optimization were performed on 10X Visium platform, including tissue sectioning, H&E staining, tissue permeabilization, fluorescent cDNA synthesis and imaging, according to the manufacturer's protocol. cDNAs of samples were sequenced on Illumina novaseq platform and were subjected to 150 cycles of paired-end (2 × 150 bp) sequencing