GSM6329499: RAI16 KO NIH3T3 cells rep1; Mus musculus; RNA-Seq
Total RNA was extracted using the TRIzol reagent according to the manufacturer's protocol. RNA  purity and quantification were evaluated using the NanoDrop2000 spectrophotometer (Thermo   Scientific, USA). 1 ug of total RNA was used for the construction of sequencing libraries. RNA libraries were constructed using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer's  instructions.