GSM5515179: D7_Punch_3; Mus musculus; ATAC-seq
Skin were trypsinized to extract basal stem cells, which were then FACS-sorted to isolate populations of interest. Freshly sorted cells (15K-100K) were pelleted and incubated with cold lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630). After removing lysis buffer, samples were immediately subjected to a transposition reaction at 37°C for 30 min with 2-10μl TnD transposase enzyme (Illumina Nextera DNA Preparation Kit). Transposed DNA was purified using QIAGEN MiniElute PCR purification kit and PCR amplified with 10-15 cycles.