Genome sequencing of Capsella bursa-pastoris: pooled lel F2 plants
DNA of 46 (23 wild-type and 23 lel) plants was extracted from frozen at -20C leaves using CTAB method (Doyle and Doyle, 1987). 65 ng of DNA of each plant was taken and mixed forming wild-type and mutant pools separately. Sequencing libraries were prepared using TruSeq DNA Sample Preparation Kits (Illumina, San Diego, CA, USA) according to manufacturer instructions. Libraries were sequenced on Illumina HiSeq 2000 with a 100-bp paired reads.