16s rRNA-seq of mus musculus: adult female cecal contents
Microbial DNA was extracted from the cecal contents using a DNA Isolation Kit according to manufacturers protocols. The V3-V4 regions of the 16S rRNA genes were amplified. Amplicons were purified using the through OMEGA Cycle Pure Kit and Monarch DNA Gel Extraction Kit. The tags were sequenced on the Illumina HiSeq 2500 PE250 platform. After merging paired-end reads, reads were performed by quality filtering and chimera detection. High quality reads were clustered into operational taxonomic units with a 97% similarity using UPARSE, and representative sequences for each OTU were screened using QIIME2.