GSM5268234: ISC_duodenum_sham replicate #2 [B1 Duo Sham]; Mus musculus; RNA-Seq
Total RNA was isolated from Lgr5+ cells, which were sorted by GFP expression, were using the Total RNA Purification Kit (Norgen Biotek). RNA was quantified with the Nanodrop 2000 (ThermoFisher), and RNA integrity was assessed by the Agilent 4200 Tapestation (Agilent Technologies). Libraries were prepared using NEBNext Ultra II Directional Library Prep Kit (New England Biolabs, Ipswich, MA, USA). Sequencing was performed on the NextSeq500 platform (Illumina; single end; 85 nt) at the Biotechnology Research Center at the Cornell University (Ithaca, NY, USA).