GSM5643710: T1; Mus musculus; RNA-Seq
Lumbar spinal cord of EAE mice and liraglutide treated EAE mice were removed on disease peak, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Agilent 2100 Bioanalyzer was used to evaluate the quality of RNA. Oligo(dT) magnetic bead was used to purify the mRNA in total RNA, then mRNA was split into 300 bp fragments and reverse transcribed into cDNA library for sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols