GSM5351202: Neonatal Rat Cardiac Fibroblast - Treatment rep 2; Rattus norvegicus; RNA-Seq
Maxwell® RSC simplyRNA Cells (Promega) with inclusion of DNAse treatment step. RNA samples were quantified using NanoDrop™ One Spectrophotometer (Thermo Scientific) and analyzed for integrity using Agilent 4200 TapeStation. Levels of remaining DNA were checked using Qubit fluorometer (Invitrogen). DNA amounts did not exceed 10% of the total amount of Nucleic Acid. RNA libraries were prepared for sequencing using standard Illumina protocols