GSM6267736: neocortex, huFOXP2, L5-6, rep1; Mus musculus; RNA-Seq
Timed-pregnant CD-1 mice were used for in utero electroporation. Using day of vaginal plug as embryonic day 0.5, dams were anesthetized at E14.5, the uterine horn was exposed, and either empty plasmid or human/mouse FOXP2 cDNA and Gfp cDNA were electroporated into the ventricular wall (IUE refs from lab). At postnatal day 7, electroporated mice were sacrificed, their brains extracted into cooled aCSF, and then sectioned sagittally at 300µmin cooled aCSF. Sections were then microdissected, pooling for each mouse its neocortical layers 5-6 (infragranular, GFP-negative cells), L4 (granular, GFP-positive cells), and layers 2-3 (infragranular, GFP-negative cells), similar to previous techniques (Fertuzinhos, 2014). Illumina TruSeq