GSM4727499: Control-AOM-DSS_rep3; Mus musculus; RNA-Seq
Colonic epithelium cells were isolated by incubating in HBSS with 2.5mM EDTA and 1mM DTT for 20min at 37℃ under slow rotation. RNA was isolated using the standard TRIzol protocol. Stand-specific libraries were constructed using the TruSeq RNA sample prepartion kit (Illumina).