GSM5776889: pre-treatment PBMC sample gd TCR; Homo sapiens; OTHER
On the date of experiment, the cells were thaw using a water bath at 37°C and loaded into Chromium microfluidic chips and barcoded within a 10× Chromium Controller (10X Genomics, US). For transcriptome, procedures were performed with reagents: Chromium Next GEM Single Cell 5' Library & Gel Bead Kit v1.1 (10X Genomics, Cat. No. 1000165). TCR enrichment was carried out using the Chromium Single Cell V(D)J Enrichment Kit, Human T Cell (10X Genomics, Cat. No. 1000005) for αβ transcripts, or customer primers for γδ TCR transcripts