GSM5639173: Normal_B1 (RNA-seq); Mus musculus; RNA-Seq
Harvested spleens were mechanically dissociated to form a single cell suspension. Erythrocyte lysis was carried out using ACK buffer and B cells were immunomagnetically selected from the resulting cell suspension using the MACS B cell Isolation Kit. Cells were washed in cold PBS and total RNA was extracted using Qiagen RNAeasy kit. RNA-seq libraries were prepared using the TruSeq RNA Sample Prep Kit v2 (Illumina).  Droplet-based scATAC-seq optimized for capture of mitochondrial DNA (mtscATAC-seq) described in Lareau et al. (PMID 32788668)