GSM5550233: Thymic DC untreated_rep2; Mus musculus; RNA-Seq
Mouse thymi were harvested, enzymatically digested (DNase and Collagenase II), and pooled. RNA was harvested from FACS purified DCs (CD45+ MHC11+ CD11c+) using Qiagen RNeasy Micro Kit and quanitifed using Aligent Tapestation. RNA libraries were prepared for sequencing using SMART-Seq v4 Ultra Low Input RNA Kit (Takara) and standard Illumina protocols