NextSeq 500 sequencing; Stress and neuronal activity during a critical period regulate odorant receptor expression in Drosophila Melanogaster
Stress and neuronal activity during a critical period regulate odorant receptor expression in Drosophila Melanogaster
50 antennae were handpicked either immediately or after 4 or 14 days Total RNA was extracted using TRIzol (Invitrogen, cat. no. 15596-018) according to the manufacturer's instructions. DNA was degraded using the Invitrogen TURBO DNA-freeTM Kit. After DNAase treatment, TRIzol RNA extraction was repeated a second time. Using 1–5 μg total RNA for each sample, we performed two rounds of mRNA isolation using the NEBNext@ Poly(A) mRNA Magnetic Isolation Module (E7490) according to the manufacturer's instructions. Libraries were generated using the NEBNext RNA ultra II RNA Library Prep kit