ChIP-Seq of Homo sapiens: healthy donor T cells
Healthy donor (HD) T cells were stimulated with CD3/CD28 Dynabeads for 24 hours. T cells were treated with vehicle (DMSO) or PLX2853 (10 nM) for 24 hours. Evaluation was performed on BRD4 binding, RNA polymerase II (Pol II) occupancy and H3K27Ac (BRD4-associated histone mark. Cells were processed for chromatin immunoprecipitation (ChIP) per Active Motif kit instructions (Active Motif). ChIP sequencing (ChIP-seq) was performed using FactorPath ChIP-Seq technology by Active Motif.