RNA-seq of mus musculus: adult bone marrow stromal cells
Cell suspension from digested leg bones were pooled together with flushed BM fraction after CD45+ leukocyte depletion. The RNA was extracted using RNeasy Mini Kit (QIAGEN) and DNA was removed using RNase-Free DNase Set (QIAGEN). RNA-seq was performed by BGI Genomics (Hong Kong). Library selection: poly-A selection method.