GSM5227634: Col-0_R3; Arabidopsis thaliana; RNA-Seq
Total RNA was isolated using EZNA Plant RNA kit (Omega) following the manufacturer's protocol. RNA samples were treated with DNase I (Roche) to remove genomic DNA contamination. Total RNA was assayed for quality control using an Agilent Bioanalyzer 2100. Libraries were prepared using TrueSeq Stranded total RNA with Ribo-Zero Plant kit following manufacturer protocols. Then, libraries were sequenced on an Illumina HiSeq2000 sequencer generating a minimum of 40 million 50bp single-end reads.