Illumina HiSeq 2500 paired end sequencing; Ultra-high-density QTL markers mapping for seedling photomorphogenesis mediating Arabidopsis establishment in southern Patagonia
Ultra-high-density QTL markers mapping for seedling photomorphogenesis mediating Arabidopsis establishment in southern Patagonia
One plant from each RIL or parental line was selected for DNA extraction Plant growth was under standart Arabidopsis culture conditions. Seeds were stratified in 0.1% agarose at 4°C 4 days. After germination, seedlings were transplanted to pots in a growth chamber at 23°C and long day (16/8 hs day/night). Total DNA was extracted according to Cao et al. (2011) \"Whole-genome sequencing of multiple Arabidopsis thaliana populations\", https://doi.org/10.1038/ng.911 Libraries were prepared in the Gregor Mendel Institute of Molecular Plant Biology, using the Tn5 transposase protocol outlined in Pisupati et al. (2017) \"Verification of Arabidopsis stock collections using SNPmatch, a tool for genotyping high-plexed samples\", https://www.nature.com/articles/sdata2017184