GSM5822299: PEF treated with insulin for 72 h_rep1; Sus scrofa; RNA-Seq
Total RNA was isolated from the cells at hours 0, 48, and 72 post-insulin treated using RIzol RNA extraction reagent (Thermo Fisher Scientific), following the manufacturer's protocol. The concentration and purity of total RNA were assessed on Nanodrop 2000 (Thermo Fisher Scientific), and the integrity of total RNA was evaluated with 2% agarose gel electrophoresis and a RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA). rRNA was removed using a Epicenter Ribo-zero rRNA Kit (Epicenter). Sequencing libraries were generated using the NEBNext UltraTM RNA Library Prep Kit for Illumina (number E7530L, New England Biolabs). RNA libraries were prepared for sequencing using standard Illumina protocols