200890C_C_1
The whole exome library was prepared using Agilent SureSelect Human All Exon V6 kit (Agilent Technologies Inc.) according to manufacturer's standard protocol. Briefly, qualified genomic DNA was fragmented to an average size of 200bp. The end of the DNA fragment were subjected to an end repair process, followed by A-tailing and the ligation of the adapters. DNA fragments with ligated adapter on both ends were selectively enriched by PCR. After PCR, Library Hybridization and exome capture were performed using biotin labeled probe and Magnetic bead selection. Captured libraries were enriched and tagged by PCR to prepare for sequencing. The final libraries were quantified using KAPA Library Quantification kit (KAPA Biosystems, South Africa) and an Agilent 2100 Bioanalyzer. Paired-end sequencing (2  150 basepair) was performed on an Illumina NovaSeq 6003 sequencer (IlluminaUSA).