GSM4777890: DT_CD45_plus_S8_2W; Mus musculus; RNA-Seq
Single cell suspensions were prepared from lungs of and stained for CD45 and DAPI. DAPI-negative/CD45+ cells were sorted via fluorescence-activated cell sorting (FACS). 10x Genomics Chromium was employed to add barcoded primer gel beads to each single cell.  The captured polyA mRNA from single cells was reversely transcribed, barcoded and amplified following standard protocol of10X Genomics Chromium Single-cell 3' Library RNA-Seq Assays , followed by sequencing in Illumina NovaSeq6000 at the Genomics and Epigenetics Core Facility at Weill Cornell Medicine.