RNAseq of Capra hircus abomasal mucosal biopsies
High-quality RNA from all samples was processed for the preparation of cDNA libraries using an Illumina TruSeq RNA sample prep kit for mRNA analysis following the Illuminas protocols. After quality control and quantification, cDNA libraries were pooled in groups of 6 and sequenced on 5 lanes on the HiSeqTM 2000 (Illumina NEB, USA) to obtain approximatively 30 million reads (100 bp paired-end) for each sample with insert sizes ranging from 200 to 400 base pairs.