GSM5822245: siDIC-DRO Induced Replicate 3; Homo sapiens; RNA-Seq
Total RNA was isolated using the RNeasy Kit (Qiagen), according to the manufacturer's instructions. mRNA-seq indexed library preparation was performed starting from total mRNA (Illumina, TruSeq Stranded mRNA) according to the manufacturer's instructions. Library quality and quantity was assessed on the 2100 Bioanalyzer High Sensitivity DNA kit (Agilent), quantified on Qubit dsDNA HS Assay, normalized and pooled to perform a multiplexed sequencing run. Clusters were generated on the Illumina flow cell and sequencing was carried out on NextSeq 550 System with paired-end 75 bp.