GSM6166018: nonInfected_HBEday4_totalRNA; Homo sapiens; RNA-Seq
RNA was isolated from Vero CCL-81 cells (non-infected and post 48h infection) using TRIZOL reagent (Invitrogen). RNA fragmentation followed by m6A RIP was performed with 15ug of RNA spiked-in with 9 ng of Bacterial RNA. Epithelial cultures were washed in cold PBS and then lyzed in 150 µL of Trizol reagent (Thermofisher Scientific) added to the apical side of the insert. RNA was purified using the Direct-zol miniprep kit (ZR2080, Zymo Research), according to the manufacturer's specifications. Sequencing libraries were prepared using SMARTer Stranded Total RNA-Seq Kit v2 (Pico Input Mammalian) from Takara and single-end sequenced (1X 88 bp) on Illumina NextSeq 2000 and 550 platforms.