Single-cell RNA-sequencing
he Illumina scRNA-seq libraries were prepared following the 10x Genomics protocol. Briefly, enzymatic fragmentation and size selection were used to optimize the cDNA amplicon size. TruSeq Read 1 (the read 1 primer) was added to the molecules during cDNA generation. P5, P7, a sample index, and TruSeq Read 2 (the read 2 primer) were added via end repair, A-tailing, adaptor ligation, and PCR. The final libraries were sequenced on an Illumina NovaSeq 6000 platform.