GSM5583331: 75-rmec-6_GTCCGC; Rattus norvegicus; RNA-Seq
Cell pellets were collected by centrifugation and resuspended in 5 mL DMEM/F12. The suspension was loaded onto a 40μm nylon filter to eliminate stromal cells and collect mammary ductal fragments, consisting of an enriched mammary epithelial cell population. To isolate RNA, cells were homogenized in TRI Reagent (Ambion), followed by RNA extraction using the MagMAX-96 for Microarrays Total RNA kit (Ambion). RNA was extracted using the RNeasy Mini Kit (Qiagen). The fastq files were processed by the UW Biotech center.