Illumina HiSeq 4000 paired end sequencing; Transcriptome sequencing of Acetabularia acetabulum caps
Total RNA was isolated from the apical end of the cells using the Single Cell RNA Purification Kit” (NORGEN BIOTEK CORP, Canada). ERCC RNA Spike-In Mix I (ThermoFisher Scientific, Massachusetts, USA) was added before mRNA enrichment. mRNA enrichment was performed using NEXTflexTM Poly(A) Beads (BIOO Scientific Corporation, Texas, USA) before library preparation with the NEXTflex Rapid Directional qRNA-Seq Library Prep kit for Illumina sequencing (BIOO Scientific Corporation, Texas, USA).