GSM5352171: PSC-Control-3; Mus musculus; RNA-Seq
Primary periosteal stem cells were collected by FACS sorting as described previously (Debnath et al., Nature 2018). In vitro osteoclastogenesis was performed as previously reported (Tsukasaki et al., Nat Metab 2020). Total RNA was extracted from periosteal steal stem cells and osteoclasts using the Maxwell 16 LEV simply RNA Tissue Kit (Promega) according to the manufacturer's instructions. RNA libraries were prepared for sequencing using standard Ion Torrent Proton protocols