Nanopore metagenome of cultured sediment
A total amount of 8g DNA per sample was used as input material for the DNA sample preparations. Sequencing libraries were generated using Ligation Sequencing Kit (SQK-LSK109) following manufacturers recommendations. Briefly, the DNA was disrupted with the Megaruptor(Diagenode,NJ,USA), the fragment above 10 kb was screened using Bluepinpin. After end repair and addition of A tail, the barcodes were added  and then detect the fragment length. Then samples with different barcodes were mixed in equimolar amounts, the linker and template were purified. And the concentration of the DNA was measured using Qubit to complete the preparation of the DNA library.