16s of mus musculus: KO2 male feces
Sequencing libraries for each isolate were generated using universal 16S rRNA V3-V4 region primers (Klindworth et al. 2012) in accordance with Illumina 16S rRNA metagenomic sequencing library protocols. The samples were barcoded for multiplexing using Nextera XT Index Kit v2. Sequencing was performed on an Illumina MiSeq instrument using a MiSeq Reagent Kit v2 (500-cycles).