Metagenomic sequencing library of coastal marine sediments from Mai Po Nature Reserve: depth 20-25cm
The MP5 samples were obtained from Mai Po Nature Reserve (Hong Kong) on 12 September 2014. This subsurface sediment samples were collected from a site covered with mangrove forest at depth intervals of 2025 cm. After collection, bulk sediments were immediately sealed in plastic bags, placed in a precooled icebox and transported to the laboratory within 4 h. For each sample, DNA was extracted from 5 g sediment using PowerSoil DNA Isolation kit (Qiagen), according to the manufacturers protocol.