Genome skimming: Astragalus pattersonii - JLMC 9805-2
Fragmentation with Bioruptor Pico sonicator using 6 cycles of 30 s On/90 s Off; NEBNext Ultra II DNA Library Prep Kit with no size selection and half volumes, 8 PCR cycles using NEBNext Dual Index Primers i701 and i505; Index Read 1: ATTACTCG, Index Read 2: CTTCGCCT