GSM4820293: E_768_H3K27me3_ChIP; Elaeis guineensis; ChIP-Seq
Chromatin was isolated, sheared, and immunoprecipitated (Sarpan et al., 2018). ChIP-grade antibodies, anti-H3K4me3 (ab12209) and anti-H3K27me3 (ab6002) (Abcam, UK), were used for the ChIP assay. Sequencing libraries were prepared from non-immunoprecipitated and ChIP DNA using the TruSeq® ChIP library preparation kit (Illumina, USA).