GSM5515732: WT 2; Danio rerio; RNA-Seq
Livers were dissected from 18-month-old adult zebrafish. Single cell suspensions were generated as described in Materials and Methods. Cells were dissociated and digested using standard collagenase and DNAse protocols, and single cell suspensions were filtered through a 70um filter. Suspensions were loaded into 10X Chromium gel beads. Library construction was performed as per 10X Genomics v3 chemistry kit. 10X chromium