GSM5350562: REM22, dilution 1:4, set 2, replicate 2; Homo sapiens; RNA-Seq
RNA was extracted using Qiagen RNeasy Plus Mini Kit (Qiagen) including DNase treatment. RNA samples were sent to Sequencing Unit of Finnish Institute for Molecular Medicine (FIMM, Helsinki, Finland) for further RNA quality control and sequencing. RNA quality assessment, library preparation for sequencing, sequencing of RNA amplicons, and data analysis were done according to manufacturer´s instructions (Nordberg et al. 2020). QIAseq Targeted RNA panel Human Molecular Toxicology Transcriptome (Qiagen) of 386 genes was used for first strand synthesis, molecular barcoding, gene-specific amplification, sample indexing, and library preparation for targeted RNA sequencing according to manufacturer´s instructions.