GSM5841343: gd85_CON replicate 2; Ovis aries; RNA-Seq
Total cellular RNA was isolated from cotyledon samples using Trizol reagent and purified using a PureLink Mini RNA kit (Invitrogen, Thermo Fisher Scientific, Waltham, MA) according to manufacturer instructions. Library preparation was conducted using the NEB Next Ultra II Library Prep Kit for Illumina according to manufacturer instructions.