metagenome seq of adult gut
Viral nucleic acid was extracted from 200l of the filtrate using the High Pure viral nucleic acid kit (Roche Diagnostics, USA) following the standard protocol. Viral DNA was amplified using rolling circle amplification (RCA) with Illustra TempliPhi 100 amplification kit (GE Healthcare, USA). The RCA products were used to prepare a 250 bp insert DNA library for each sample following the manufacturers standard protocol.