GSM5203011: WT LSK 2 [ChIP]; Mus musculus; ChIP-Seq
LSK HSPCs were isolated by fluorescence-activated cell sorting and subjected to Nuclei extraction using NP40 buffer and digested into nucleosomes using Mnase. ChIP-seq libraries of fragmented DNA were prepared using the KAPA Hyper Kit (KAPA) according to the manufacturer's instructions.