GSM5550426: Pole4KO_RNAseq_rep2; Mus musculus; RNA-Seq
eSPAN in mESC was performed exactly as previously described (Li et al., 2020).Total RNA samples were purified the same as for RT-PCR. RNA-seq libraries were prepared and deep sequencing was performed by Columbia University Genome Center. For CUT&TAG and eSPAN libraries, the supernatants were purified with ChIP DNA concentrator columns and library PCR was performed using standard Illumina Nextera Dual Indexing primers. Samples were pooled and sequenced using paired-end sequencing on Illumina NextSeq 500 platforms at Columbia University Genome Center.