RNA Seq of Stevia rebudiana
First-strand cDNA was generated using random hexamer-primed reverse transcription and purified using Ampure XP Beads (Agencourt, Massachusetts, USA), before proceeding to the synthesis of second-strand cDNA. The double-stranded complementary DNAs (ds-cDNAs) were then purified, followed by 3 end reparation and single nucleotide A (adenine) addition. Sequencing adaptors were ligated to the 3 adenylated cDNA fragments and purified using Ampure XP beads (Agencourt, Massachusetts, USA), and suitable fragments were size-selected, purified and amplified. Agilent 2100 Bioanalyzer was used to quantify and qualify the libraries.