Illumina HiSeq 2000 paired end sequencing; Nucleosome positioning in H3S57 mutants in yeast
Nucleosome positioning in H3S57 mutants in yeast
1.2 x 109 yeast spheroplasts were digested with micrococcal Nuclease (MNase), 2.4 units at 37ºC for 30min with 3mM CaCl2. The reactions were stopped by addition of EDTA to a final concentration of 0.02 M and subsequently incubated with RNase A (0.1 mg) for 4h at 37ºC and further treated with Proteinase K at 65ºC o/n.  Normal growth condition No treatment DNA was purified using phenol–chloroform extraction and concentrated by ethanol precipitation. Sample prep following Illumina standard Protocol