GSM6268258: H3K27ac_NejireKD_rep2; Drosophila melanogaster; OTHER
CUT&Tag experiments were performed as in Kaya-Okur, H.S., et al. 2019. Embryos were fixed with 1%PFA. 50 hand-staged embryos were used per sample. DNA was purified and 1pg of tagmented lambda phage genome was added to each sample as spike-in for quantification before library preparation. Libraries were prepared by amplification of tagmented DNA fragments using NEBNext® Q5® HiFi PCR Master Mix (NEB, Cat. M0543). Primer sequences were taken from Buenrostro et al., 2015. Libraries were quality controlled by capillary electrophoresis on the Fragment Analyzer system (Advanced Analytical).