RNA-Seq of Spongospora subterranea
The purified spores were divided into two parts. The first part was suspended in Hoaglands solution and incubated at 25 C to stimulate the germination of resting spores. The second part was also incubated at 25 C and served as an ungerminated control group. Total RNA was extracted from each sample. Library construction was generated using the Illumina TruSeq Stranded mRNA Library Preparation Kit TruSeq and then sequenced on a NovaSeq 6000 instrument at Macrogen, Inc.