GSM4777782: T-A2iL-P9-1; Mus musculus; OTHER
Genome DNA was prepared using DNeasy Blood & Tissue Kit (QIAGEN, Valencia, CA) For population cells, paired-end DNA library were prepared according to manufacturer's instructions (Agilent). Genomic DNAs (gDNA) from cell samples were sheared into 180$280 bp fragments by Covaris S220 sonicator. Ends of gDNA fragments were re- paired and 30 ends were adenylated. Both ends of gDNA fragments were ligated at the 30 ends with paired-end adaptors (Illumina) with a single 'T' base overhang and purified using AMPure SPRI beads from Agencourt. The adaptor-modified gDNA fragments were enriched by six cycles of PCR using SureSelect Primer and SureSelect ILM Indexing PreCapture PCR Reverse Primer. The concen- tration and size distribution of the libraries were determined on an Agilent Bioanalyzer DNA 1000 chip.