GSM5778100: M15.133; Macaca mulatta; RNA-Seq
RNA was isolated from blood samples stored in Paxgene tubes at the Yerkes National Primate Center for library preparation (http://www.yerkes.emory.edu/nhp_genomics_core/). RNA quality was assessed using an Agilent 4200 TapeStation and concentration via the RNA HS assay on the Qubit. Globin transcripts in the blood RNA were blocked with the FastSelect Globin Reagent (Qiagen) prior to library preparation. Libraries were prepared using the Clontech SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio) in combination with the NexteraXT DNA Library Preparation kit to append dual-indexed adapter sequences (Illumina).  Libraries were validated by capillary electrophoresis on an Agilent 4200 TapeStation, pooled at equimolar concentrations, and sequenced on an Illumina NovaSeq6000 at 100SR, yielding 25-30 million reads per sample.