GSM5196834: IL_6h_2; Mus musculus; RNA-Seq
RNA was harvested using Trizol reagent. Library concentration was first quantified using a Qubit 2.0 fluorometer (Life Technologies), and then diluted to 1 ng/μl before checking insert size on an Agilent 2100 and  quantifying to greater accuracy by quantitative PCR (Q-PCR) (library activity >2 nM). RNA libraries were prepared for sequencing using standard Illumina protocols