GSM5417992: RNA-seq AB1 Ts8 rep1; Mus musculus; RNA-Seq
Total RNA of WT and trisomic mESCs was isolated following TRIZOL RNA extraction method, and poly(A) based mRNA enrichment was performed. Then mRNA was fragmented and cDNA was synthesized from mRNA library. RNA-seq library was prepared following DNA end repair, dATP tailing, adapter ligation and PCR.