16S rRNA gene sequencing of mouse microbiome
The bacterial 16S ribosomal RNA gene V3-V4 region was amplified using forward primers containing the sequence CCTACGGGNGGCWGCAG and reverse primers containing the sequence GACTACHVGGGTATCTAATCC. DNA libraries were run on an Illumina MiSeq. Sequencing was performed with a 2 x 250 paired-end configuration.