RNA sample taken from Escherichia coli K-12 MG1655 growing in M9 glucose medium supplemented with 30 g/L L-methionine
Transcript libraries were prepared using the MGIEasy RNA Library Prep Set. The protocol was initiated at the Elute, Prime, Fragment step using 100400 ng of rRNA-depleted RNA and followed until the completion of the Enrich DNA Fragments step. Libraries were quantified using a Qubit Fluorometer and size distribution assessed on a Bioanalyzer. Libraries were 100bp paired-end sequenced on a DNBSEQ platform at BGI-Europe.