DNA-Seq of Escherichia coli JW5503 at exponential phase
The genomic DNA was extracted from exponential phase culture in 2xYT medium using the UltraClean Microbial DNA Isolation Kit according to the manufacturers instructions (Qiagen). The library was prepared using the Illumina TruSeq Nano library preparation and sequenced using the 2x 250 paired-end Illumina MiSeq platform version 2.