Illumina HiSeq 3000 sequencing; ATAC-seq analysis of shCtrl and shAML1-ETO Kasumi-1 cells
ATAC-seq analysis of shCtrl and shAML1-ETO Kasumi-1 cells
105 cells were washed once in 50μl PBS and resuspended in a transposase reaction mix containing 12.5 μl 2 × TD buffer, 2 μl transposase (Illumina), 10.5 μl nuclease-free water and 0.01% NP-40. Tagmentation was performed for 30 min at 37 °C. The optimum number of amplification cycles was estimated by qPCR reaction. Fragments larger than 1,200 bp were excluded by SPRI size selection. DNA concentration was measured using a Qubit fluorometer (Life Technologies). Libraries were amplified using custom Nextera primers. Nextera DNA Library Prep Kit