Illumina HiSeq 2000 paired end sequencing; Expression profile of third instar larvae tissues from wild type and bsAS mutant flies
Expression profile of third instar larvae tissues from wild type and bsAS mutant flies
Third instar (L3) and late pupa (LP) imaginal discs were manually dissected in PBS. Flies were grown at 25ºC according to standard protocols. RNA from imaginal discs was extracted with ZR-RNA MicroPrep Kit from Zymo Research following the manufacturer's instructions. Sequencing libraries were prepared using TruSeq Stranded mRNA Library Preparation Kit from Illumina and following the manufacturer's instructions.