GSM4822429: 24hr HRVA - 5398; Homo sapiens; RNA-Seq
RNA was purified from sample lysates using the Quick-RNATM MiniPrep kit (Zymo Research). Lysates were centrifuged at 16,000xg for 2 minutes in a QIAshredder (Qiagen) to homogenize sample and remove insoluble debris before beginning standard manufacturer protocol with inclusion of optional DNase step. RNA was eluted in 30 mL of DNase/RNase free water and eluent was passed through the column one additional time to ensure maximum RNA recovery from filter. KAPA Biosystems mRNA HyperPrep whole transcriptome libraries (Roche) were constructed with 250 ng RNA per sample and 12 cycles of library amplification. Libraries were barcoded using Illumina Dual-Index adapters (Integrated DNA Technologies) and paired-end sequenced using the Illumina NovaSeq® 6000 system.