PacBio HiFi Reads from HG002 Ultra Low DNA Input Library
Frozen cell pellets were purchased from Coriell (NIST ID = HG002). gDNA was extracted with the Lucigen Masterpure kit. gDNA was sheared to 10kb with Megarupter1. 5 ng of sheared DNA was processed according to Preparing SMRTbell Libraries from Ultra-Low DNA Input from PacBio using SMRTbell gDNA Sample Amplification Kit and SMRTbell Express Template Prep Kit 2.0. Libraries were size selected to 8-17kb with BluePippin and sequenced on Sequel II System. In SMRTLink v9.0, HiFi reads were generated with Circular Consensus Sequencing, adapter sequences were trimmed with Trim PCR Adapters and PCR duplicated reads were removed with Mark PCR Duplicates.