GSM5516186: HET2 adult kidney; Mus musculus; RNA-Seq
Total individual kidney RNA was extracted using anRNeasy Maxi kit75162 Lot.260018727/ Lot.160012031 from Quiagen according to manufacturer's instructions. The integrity of RNA was assessed using a chip-based capillary electrophoresis machine and RNA concentration determined using a full spectrum (220-750nm) spectrophotometer. The quality control of the RNA was additionally checked with RNA 6000 Pico de Agilent Technologies, according to the manufacturer's instructions. To obtain two independent total RNA preparations from the two different conditions (wild type: WT1 & WT2; Tshz3+/LacZ: HET1 & HET2) we pooled RNA from 3 kidneys per group in the same proportion. The starting material (1µg Total mRNA, dissolved in RNase-, DNase- and protease-free molecular grade water) was sent to GATC (Eurofins) for sequencing (Genome Sequencer Illumina HiSeq). DNA and mRNA libraries were prepared for sequencing using standard Illumina protocols