Hi-C seq of Aldabrachelys gigantea
The Hi-C library was constructed with a 250 l whole blood sample that was first fixed with  1% formaldehyde for 15 min at room temperature. Then, solid glycine powder was added to obtain a final concentration of 125 mM and incubated for 15 min at room temperature with periodic mixing. After centrifugation, the pellet was resuspended in PBS + 1% Triton-X solution and incubated at room temperature for 15 min. Then, the nuclei were collected after the mixture was spun down. The cross-linked sample was sent on dry ice to Phase Genomics (Seattle, Washington, USA)  for sequencing. Hi-C library was generated using the Phase Genomics Proximo Animal kit version 4.0. Briefly, the DNA sample was digested with DpnII, 5-overhangs were filled while incorporating a biotinylated nucleotide, the resulting blunt-end fragments were ligated, sheared, and the biotinylated ligation junctions were captured with streptavidin beads, and the resulting fragments were sequenced on a NovaSeq 6000 150 bp paired-end run.