GSM4779207: Epidermis_DKO_6mo_rep2; Mus musculus; RNA-Seq
After euthanization, dorsal skin samples of 2cm2 were collected. The dermal side of skin was then placed in a sterile culture dish in 60ºC water bath for 1 minute. Sterile forceps and scalpels were used to separate the dermis from the epidermis. The epidermis was collected and homogenized in TRIzol reagent (Invitrogen), and enriched by removing rRNA with RNaseH. The purified mRNA was fragmented into short fragments. The cDNA was generated using random hexamer-primed reverse transcription, and assessed using Agilent 2100 BioAnalyzer (Thermo Fisher Scientific). The cDNA libraries were sequenced on the BGISEQ-500 platform (BGI-Shenzhen) with 50 single-end read.