GSM5550062: SGC0946_BMDM_M1; Mus musculus; ChIP-Seq
Briefly, 10-20 million BMDMs were lifted using TrypLE (ThermoFisher) and crosslinked with 1% formaldehyde (ThermoFisher) for 10 min. Chromatin was pulled down overnight using an anti-H3K79me2 antibody (Clone: NL59, Merck). Illumina libraries were created from 10 ng ChIP chromatin using the KAPA HyperPrep (Roche) kit.  Libraries were pooled to a concentration of 10 nM and sequenced single-ended on an Illumina HiSeq 4000 instrument (Illumina) to a depth of ±40 million 50 bp reads per sample.