GSM5515897: Top Internode section of the maize stem, rep 2; Zea mays; RNA-Seq
Total RNA from female inflorescences (FI) and top internodes of the stem (TI) at 60 DAS were extracted using the PureLink RNA-micro-to-midi Kit (Invitrogen Corp., Carlsbad, CA, USA) as described by the manufacturer. The purity and integrity of RNA samples were evaluated by electrophoresis on 2% RNase-free agarose gels, spectrophotometry (A260/A280 and A260/A230 ratios) using a NanoDrop 2000 (Thermo Scientific) and quality analysis using the Agilent 2100 Bioanalyzer system (Agilent technologies, Inc, USA). RNA libraries were prepared for sequencing using standard Illumina protocols