Illumina HiSeq 3000 paired end sequencing; Single-cell sequencing of epicardial cells and fibroblasts within the infarcted heart
Single-cell sequencing of epicardial cells and fibroblasts within the infarcted heart
mice were anaesthetized (isoflurane 1.5%) and the left anterior descending coronary artery (LAD) was ligated for 50â€‰min followed by reperfusion. LAD occlusion was controlled by ST-segment elevation in electrocardiography recordings. Sham operated mice underwent surgical incision without ligation. 10X Genomics 3' Single Cell Kit v2  10X Genomics 3' Single Cell Kit v2