Illumina HiSeq 2500 paired end sequencing; Transcriptome analysis of Beijerinckiaceae bacterium RH AL1 grown with pyruvate as the carbon source in response to lanthanum supplementation
Transcriptome analysis of Beijerinckiaceae bacterium RH AL1 grown with pyruvate as the carbon source in response to lanthanum supplementation
Biomass was collected by centrifugation (10000 x g, 10 min, at room temperature). Pelleted biomass was flashfrozen and kept at -80 °C before being processed. Beijerinckiaceae bacterium RHAL1 was grown to exponential phase in minimal medium containing pyruvate as carbon source (0.2 % [w/v]) with and without lanthanum (1 µM) as growth supplement. Total RNA was extracted following a protocol modified after Wegner et al., 2013 (doi: 10.1016/j.margen.2012.12.001). Total RNA was enriched for mRNA by rRNA depletion using the MICROBExpress  bacterial mRNA enrichment kit (Thermo Fisher Scientific, Schwerte, Germany).  RNA-Seq libraries were prepared using the NEBNext Ultra II directional RNA library prep kit for Illumina (New England Biolabs, Frankfurt, Germany).