GSM5516241: hbdTSC_E9_rep2 [RRBS]; Homo sapiens; Bisulfite-Seq
DNA was isolated from samples and incubated in lysis buffer (25mM Tris-HCl at pH8, 2mM EDTA, 0.2%SDS, 200mM NaCl) supplemented with 300μg/mL proteinase K (Roche) followed by phenol:chloroform extraction and ethanol precipitation. hiTSC colonies and hbdTSC colonies were passaged twice on matrigel in order to eliminate the presence of MEF feeder cells. Libraries were prepared as previously described in Boyle et al., 2012.