GSM5598695: IL-13+VitD H3K9Ac; Homo sapiens; ChIP-Seq
For RNA isolation, cells were collected in Tripure and RNA was expracted by Quick-RNA MicroPrep Kit (Zymo Research). For ChIP, cells were fixed in 0.8% PFA and sonicated by Covaris sonicator. ChIP was performed in IP-Star Compact automation system (Diagenode) Illumina standard protocol