GSM6052379: R787G_1_9 hrs auxin; Saccharomyces cerevisiae; RNA-Seq
Cells were pelleted and RNA was extracted using SDS and hot acid phenol, followed by chloroform. RNA was recovered by and ethanol precipitation. RNA integrity was checked on a 1.2% agarose gel and run on an Agilent Technologies 2100 Bioanalyzer. Samples were ribo-depleted with TruSeq Stranded Yeast Ribo-Zero Gold Kit (Illumina, MRZY1324) per manufacturer's instructions. Illumina libraries were generated using the TruSeq Stranded RNA LT kit with Ribo-Zero Gold, 48; Set A (Illumina, RS-122-2301). Libraries were normalized and pooled together with an average base pair size of 290 and sequenced as 50 bp single reads on the HiSeq 2500 (Illumina) using HiSeq Control Software v2.2.58.