GSM5582437: Untread E. faecalis rep 1; Enterococcus faecalis OG1RF; RNA-Seq
E. faecalis cells were lysed with lysozyme (30 mg/mL) and mutanolysin (500 U/mL) in Tris-EDTA (TE) buffer at 37 °C for 10 min. Total RNA was then extracted using an RNeasy Mini Kit (Qiagen Ltd., Germany) according to the manufacturer's instructions. RNA libraries were prepared for sequencing using standard Illumina protocols