GSM5778163: Evernimicin replicate A; Escherichia coli; OTHER
When the culture density reached A600 of ∼0.4 cells were harvested by rapid filtration, frozen in liquid nitrogen, and processed for ribosome profiling. The library for next-generation sequencing was prepared using size-selected RNA fragments ranging in size from 20 to 46 nt.  Replicate A samples followed the library construction protocol described in Ingolia, et al., 2009. Replicate B followed the library construction protocol described in McGlincy and Ingolia, 2017.