GSM5822503: LN18_EGCG+HC2; Homo sapiens; RNA-Seq
After 24 hours of glioma cells treatment, total RNA was using TRI Reagent® (Molecular Research Center, USA). RNase-free DNase treatment was used to remove contaminating DNA (QIAGEN), and RNA was purified using the RNeasy Mini Kit (QIAGEN). The cDNA libraries were prepared according to the protocol outlined in the TruSeq RNA sample Preparation Kit v-2 (Illumina, San Diego, USA). A total of 10-12 samples per lane were multiplexed and sequenced on an Illumina HiSeq 2500 using the paired-end cluster generation kit (Illumina).