GSM4819929: A375 parental WT replicate 1; Homo sapiens; RNA-Seq
RNA was extracted using Trizol and purified following the manufacturer's instructions.  RNA was treated with TruboDNAse.  Sequencing libraries were prepared from 1ug of purified RNA using the NEBNext Ultra II Directional RNA Library Prep Kit (NEB #E7750S) and the NEB polyadenlyated mRNA enrichment kit (NEB #E7490S) RNA libraries were prepared for sequencing using standard NEBNext Ultra II Directional Library Prep Kit protocol.