GSM6165144: 30 min-3; Mus musculus; RNA-Seq
The total RNA was purified from the lung tissues using Isogen II (NipponGene, Tokyo, Japan), according to the manufacturer's instructions. RNA (500 ng) was subjected to RNA sequencing using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina and NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (New England Bio Labs, Cambridge, UK).