Illumina HiSeq 1500 paired end sequencing; RNA-seq of two human and one cynomologous NPC line to assay activity of DNAse1 hypersensitive sites in the proximity of the Trnp1 gene
RNA-seq of two human and one cynomologous NPC line to assay activity of DNAse1 hypersensitive sites in the proximity of the Trnp1 gene
NPCs grown in cell culture were harvested and the pellet was resuspended in Trizol Column isolation using Zymo Micro prep kit For RNA-seq library construction and library preparation the prime-seq protocol was used