GSM5769176: ARDS 019_ND1; Homo sapiens; Bisulfite-Seq
Specimens stored in RNAProtect. 1:100 beta-mercaptoethanol added during thawing, cells extracted and DNA collected using QIAShredder and AllPrep columns. DNA was sheared to ~250 bp using a Covaris defice and enzymatic methyl-seq and barcoding performed using NEB Enzymatic Methyl-Seq Kit Specimens were sequenced, demultiplexed and each  aligned and counted using Bismark