GSM5351152: small RNA_HiII_3_0mm_1; Zea mays; ncRNA-Seq
TRI Reagent® (Sigma-Aldrich) follow instruction from manufacturer For sRNA library preparation, 20- to 30-nt RNAs were excised from a 15% polyacrylamide/urea gel, and ~25 ng of sRNA used for library construction with NEBNext® Small RNA Library Prep Set for Illumina® (New England Biolabs, lnc.) were used for ultra-low amount of total RNA starting from 100 ng. For RNA-seq, 2 µg of total RNA was treated with DNase I (New England BioLabs) and then cleaned with RNA Clean and Concentrator-5 (Zymo Research). The NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina® (New England BioLabs) was used for library construction with 500 ng of treated RNA.