GSM5643478: KF15_NSG_M10_SPL_LSK_PCR1; Mus musculus; OTHER
Viable cells were sorted on the BD FACSAria III directly into Eppendorf tubes. The cells were then washed twice with PBS + 0.04% BSA. Cells were then spun down and resuspended in PBS + 0.04% BSA + 0.4 U/ uL RNase Inhibitor (Roche) to a final cell concentration of >1500 cells per uL. The final cell suspension was put through a cell strainer then onto a 10X Chromium Chip