A549-GeCKO library generation and host factor screening for H5N1 virus infection
the pooled A549-GeCKO cell library-B were infected with H5N1 virus at an MOI of 3; this virus was isolated from a patient with a fatal outcome in China in 2005. Surviving cells were reseeded and expanded for two more rounds of H5N1 virus infection. Then genomic DNA was extracted from the surviving cells, and integrated sgRNAs were amplified by PCR and sequenced.There are three sets of parallel experiments in screening, this is the first repeat experiment of library-B