5p plasmid bias 4 rep 3
RNY5 mutant pools of the 3 and 5 high throughput mutagenesis analysis were generated by PCR using degenerate nucleotides. For sequencing of the different RNY5 mutant pools the Illumina adapter and index sequences were introduced by PCR. The PCR products were sequenced on a HiSeq 2500 using 50 bp single end sequencing at the Earlham Institute (Norwich Research Park).