GSM5460918: ∆ve-1_VEG_Light_R2 [MYC_ML2]; Neurospora crassa; RNA-Seq
Total RNA was isolated from frozen mycelia (approximately 100 mg) using 1g of Zirconia/Silica Beads (product number) with RLC extraction Buffer by using RNeasy Plant Mini Kit (Qiagen), following the manufacturer procedure. RNA was treated with on-column DNA digestion during RNA purification using RNAseq-free DNA set (Qiagen). Poly-A selection and cDNA libraries preparation for RNA were prepared according to the Illumina mRNA Sample Preparation Guide