GSM5227728: synuclein_7wk_3; Mus musculus; RNA-Seq
midbrain area containing dopaminergic neurons indicated by hKO1(+) neurons were micro-dissected and collected by FACS, directly into TRIzol LS. Total RNA extracted using Direct-zol RNA microprep kit (Zymo Research) and eluted in 8uL. Library preparation was prepared using SMARTer Ultra-Low RNA Kit (Clontech) and cDNA amplified according to manufacturer's protocol.