Whole exome sequencing Pancreatic neuroendocrine tumors (PanNETs) contorl (Liver).
All animal work was performed in accordance with the Animal Welfare Act and the Guide for the Care and Use of Laboratory Animals under UTSW and UAB Institutional Animal Care and Use Committee approved protocols. Bi-transgenic Ins-p25OE animals were generated from crossing of the tetOp-p25GFP strain (The Jackson Laboratory stock # 005706) with the Ins2-rtTA strain (The Jackson Laboratory stock # 008250). Breeders and pups were maintained in the absence of doxycycline to allow for normal development of offspring prior to transgene induction. Upon weaning, at 3-4 weeks of age, offspring were administered 1 mg/L doxycycline via drinking water to induce transgene expression in bi-transgenic animals. Bi-transgenic animals were co-housed with transgene negative littermates. Transgene negative littermates were used as normal controls. All mice were maintained in the C57BL/6 background. Animals were euthanized by CO2 administration and cardiac perfusion.