GSM6267740: Brunello_ACE2 293T_Spike_mNeonGreen_Presort_S2; Homo sapiens; OTHER
The genomic DNA was extracted from the sorted cells using Thermo scientific GeneJet Genomic DNA purification kit (Catalogue# K0721). Following extraction of genomic DNA, sgRNAs were amplified for seuqencing. Equal molar amounts PCR gel-purified samples were pooled, denatured and diluted to 1.8pM, of which 1.3 ml was sequenced on a NextSeq 550 using a high output 75 cycle sequencing kit (Illumina)