RNA-Seq of Aedes communis mosquitoes and their parasitic mites (Mideopsis sp.)
Samples were homogenized in pools of 10 mosquitoes or 20 mites, using ZR BashingBead 0.1mm. Total RNA was extracted from the homogenates using the ZymoBIOMICS DNA/RNA Miniprep Kit. Ribosomal RNA (rRNA) depleted RNA was extracted from each sample using the Ribo-Zero Gold kit. Whole-transcriptome libraries were constructed using DNA nanoball technology (paired-end sequencing) on a DNBseq platform