GSM4728470: Suv39h1, Suv39h2 double knock-out whole cell extract replicate 1; Mus musculus; ChIP-Seq
Flow cytometrically sorted DP thymocyte cells (CD45.2+CD19-CD4+CD8+) were fixed in 1% formaldehyde. Chromatin was Mnase digested then immunoprecipitated using anti-LaminB1 antibody. Chromatin was reverse cross-linked and purified using Zymo ChIP clean and concentrator kit. Libraries were prepared using Illumina TruSeq DNA kit. Libraries were sequenced on an Illumina NextSeq 500 to produce paired-end 81bp reads.