GSM5350657: BM sample donor3; Homo sapiens; RNA-Seq
The different cell populations (NM1s, NM2s, NM3s, NM4s and cMoPs) were sorted from BM by using a Becton Dickinson FACS Aria Fusion (BD Biosciences) and then labelled with the BD Single-Cell Multiplexing Kit (BD Biosciences), strictly following the manufacturer's protocol. Both cell viability and counts were then evaluated by the BD Rhapsody scanner, and only samples with high viability (> 85 %) were used for sequencing. Approximately 15000 cells were pooled together and loaded in a single BD Rhapsody cartridge. The procedure described above was repeated for three different BMs cDNA Libraries were prepared using the BD Rhapsody Whole Transcriptome Analysis (WTA) Amplification kit and BD Single-Cell Multiplexing kit (BD Biosciences) following the manufacturer's protocol