GSM4879170: gap90_embryo_2_2.5h_auxin_treat_rna_rep3; Drosophila melanogaster; RNA-Seq
RNA for mRNA-Seq and qRT-PCR experiments was isolated using the RNeasy Mini kit (Qiagen) with on-column DNA digestion (RNase-free DNase Set, Qiagen) according to the manufacturer's recommendations. NEBNext Poly(A) mRNA magnetic Isolation Module (NEB), followed by NEBNext Ultra Directional RNA Library Prep kit for Illumina (NEB)