GSM5822353: ACC.etr17.4h.R4.R1; Arabidopsis thaliana; RNA-Seq
Frozen samples were ground in liquid nitrogen, and RNA isolation was performed according to the Qiagen plant RNeasy kit protocol, with the addition of the Qiagen RNase-free DNase treatment. After RNA isolation, samples were quantified by A260 using a Nanodrop spectrophotometer. Each sample yielded at least 3ug, and on average 15ug, of RNA. One Col-0 ethylene 4 hour treatment had an RNA integrity number (RIN) less than 6 and was not sequenced. Library prep was performed by GENEWIZ, LLC as part of sequencing services.