ATAC-Seq of Bicyclus anynana: wounded wing
One of the forewings was poked using tungsten needle at 18 hr after pupation and the wing was dissected at 24 hrs after pupation. 20 wings were dissected and pooled for each replicate. Samples were snap frozen immediately after dissection using liquid N2 and stored in -80C until further use. ~80,000 nuclei were used for each library. Nuclei isolation and library preparation were done with little modification as described in Omni-ATAC protocol (Corces et al, 2017). Sequencing was performed at AIT Novogene, Singapore; Reads from the sample were aligned to the Bicyclus anynana (BaGv2) genome using bowtie. Peak call was done using F-seq.