Shotgun sequencing of RhCMV 180.92 virus from rhesus macaque fibroblasts
100ng of DNA extracted from infected rhesus fibroblasts was sheared in a Covaris S220 sonicator to aproximately 450bp and a sequencing library was prepared by carrying out seven cycles of PCR with indexed primers (New England Biolabs) using a Kapa LTP library preparation kit. Libraries were sequenced using a NextSeq 500  (Illumina).