GSM6268629: Primary MC38 tumor vanoxerine #1; Mus musculus; RNA-Seq
Snap-frozen tumors were mechanically disrupted and total RNA was extracted using the total RNA purification kit by Norgen Biotech Corp, following the manufacturer's guidelines. Quantification of total RNA samples was performed with a Qubit HS RNA assay (ThermoFisher Scientific) and fragment size was evaluated with a Fragment Analyzer HS NGS assay (AATI). A RNA Quality Number (RQN) of 6.0 or higher was considered satisfactory for library construction. Library construction was performed with a Truseq RNA v2 (Illumina) with unique barcodes compatible with the Illumina NextSeq 500 platform. RNA libraries were prepared for sequencing using standard Illumina protocols