GSM6165449: THP1_NC_rep2; Homo sapiens; RNA-Seq
Total RNA was isolated from THP-1 cells and H37Rv infected cell using Trizol reagent according to the manufacturer′s instructions. The total RNA were then subjected to accurate detection of RNA integrity using Agilent 2100 BioAnalyzer. To obtain mRNA, mRNA with polyadenylate tails can be enriched by magnetic beads with oligo-thymine beads attached. At last, Illumina sequencing is performed after pooling of different libraries according to the requirements of effective concentration and target unload data amount. RNA libraries for RNA-seq were prepared for sequencing using standard Illumina protocols.