single cell RNA seq of human PBMC
Isolated PBMCs were resuspended in 1x PBS (Gibco) with 0.04% weight/volume BSA.  Chromium Single Cell 3 Reagent (V2 chemistry) was used for barcoding and cDNA library preparation as per manufacturer guideline and cDNA was sequenced using the 10X Genomics Single Cell RNA sequencing platform.