GSM4728047: hHOXA11; Homo sapiens; RNA-Seq
TRIzol (iNtRON Biotechnology, Cat#17061) was used to dissolve cell components and minimize RNA degradation during cell lysis. Total RNAs were extracted with addition of chloroform (Sigma, Cat#C2432), followed by precipitation and purification. NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina® (Cat#E7760S) was used for library construction. High quality libraries were generated with1 ug of total RNA according to the instruction manual.