GSM5778032: HCC827_osi_acute_1 [RNA-seq]; Homo sapiens; RNA-Seq
Cells were lysed in RLT buffer (Qiagen) and RNA extracted using the Qiacube HT according to manufacturer's instructions, and RNA concentration quantified using the Qubit fluorometer. Illumina mRNA TruSeq library was used for library preparation. RNA-seq was sequenced on 9 lanes of Illumina HiSeq4000 with paired end 150bp reads by the CRUK Genomics Core Facility.