GSM5268472: WT_3; Danio rerio; RNA-Seq
Trunks of foxl2l:egfp;foxl2l-/- or foxl2l:egfp;foxl2l+/+ containing gonad were dissociated by 0.2% collagenase and 0.25% trypsin at 21 dpf. Around 300 EGFP+ germ cells of each genotype were sorted by fluorescence activated cell sorter. cDNA was synthesized by SMART-Seq HT kit seq (Takara Bio). cDNA library was constructed by Nextera XT DNA Library Prep Kit (Illumina Inc.) following the manufacturer's protocols.