ScRNA-seq of Mus Muculus: TAb2 treat
Single cells suspensions were obtained from tumor-bearing mice treated with PBS or anti-PD-L1 as described previously.  CD45+ immune cells were isolated using EasySep Mouse CD45 Positive Selection Kit (StemCell Technologies, Catalog# 18945) according to manufacturers instructions.  Purified CD45+ immune cell samples were submitted to the University of Colorado at Anschutz Genomics and Microarray Core for single cell capture and library preparation.  Cells were loaded into a 10Genomics Single-cell Chip G for the 3 captures.  Single-cell gene expression libraries were prepared using Chromium Next GEM Single Cell 3? Reagent Kits (v3.1: Dual Index Libraries) according to the manufacturers instructions.  Samples were sequenced on the Illumina NovaSeq 6000 platform for an estimated read depth of 100,000 reads per cell.