Illumina HiSeq 2500 sequencing; Timecourse RNA-seq of kidney organoid development in control and WT1-knockout iPSCs
Timecourse RNA-seq of kidney organoid development in control and WT1-knockout iPSCs
Samples were harvested for iPSCs prior to induction of organoid formation as well as at days 5, 7, 9, 11, 12, and 21 after induction of organoid formation (according to 10.1038/nbt.3392).Total RNA was collected. Parental Cell line:WT29-iCas9 iPSCs were infected with lentiviruses containing an expression construct for WT1 gRNA and Puromycin at a multiplicity of infection of 0.5, and were selected for 6 days.  No Doxycyline was added RNeasy Mini Kit with on-column DNase digest (Qiagen) TruSeq Illumina mRNA-seq (stranded - high input)