Leaf transcriptome of Pterocarpus dalbergioides
Five hundred ng of total RNA was used to enrich mRNA using NEB Next Poly (A) mRNA magnetic isolation module and the enriched mRNA was chemically fragmented, reverse transcribed and cleaned. The cDNA was end repaired, adapter ligated, size selected, PCR amplified (12 cycles) and cleaned prior to library construction. Library was constructed using NEBNext Ultra II RNA Library Prep Kit using manufacturers protocol, quantified using Qubit fluorometer, validated in TapeStation and sequenced in Illumina HiSeq 2000 (Illumina Inc., San Diego, CA, USA) using 150 bp paired-end chemistry.