GSM5514809: SC184; Homo sapiens; RNA-Seq
Biopsies were finely diced using a scalpel and placed in a Miltenyi C tube.  The enzymes from the Miltenyi whole skin dissociation kit, human, were used to digest cells over a period of 2 hours at 37 degrees.  Biopsies were then further mechanically separated using the h_skin_01 program on the Miltenyi OctoDissociator.  The resulting single cell suspension was filtered through a 70 micron filter, a 30 micron filter, and finally resuspended in PBS containing 0.04% BSA.  Cells were loaded into the the 10X Genomics Chromium instrument according to the manufacturer's directions. Libraries were constructed according to 10X Genomics' Single Cell 3' Reagent Kits v2 User Guides