GSM5776858: D4_PMNs_Buffer (phagocyte infection); Homo sapiens; RNA-Seq
Cells were washed with PBS. Cells were resuspended in RLT buffer (Qiagen) and vortexed for 1 min before being placed at −80°C. RNA for each donor was then isolated with the RNeasy Plus Mini Kit (Qiagen) following the protocol with on column DNAse Digestion (Qiagen). Libraries were generated for each donor using the CelSeq2 protocol (Hashimshony et al. Genome Biol 2016) and were sequenced on  Illumina NovaSeq.