GSM6268799: ATAC: DAXX/ATRX WT, Etoposide treated, replicate 1; Homo sapiens; ATAC-seq
Cells were harvested and ATAC-seq was performed in two biological replicates according to the Omni-ATAC-seq protocol. DNA was purified using a MinElute PCR purification kit (Qiagen) and eluted in 10 l Elution Buffer for library amplification PCR amplification of tagmented DNA was done using the NEBNext HiFi PCR mastermix (New England Bioloabs) with a universal forward and sample-specific reverse oligo for sample barcoding