Amplicon for Trifolium pratense-associated bulk soil microbes in 2015
Soil was sampled and stored in a -80 C freezer until DNA was extracted using a MoBio PowerSoil DNA kit. DNA yields were quantified using a PicoGreen assay and diluted to a standard DNA concentration of 6.0 ng/ul for bulk soil samples and 50.0 ng/ul for rhizosphere soil samples. DNA was amplified and sequenced by Argonne National Laboratories.