Main channel of Jinsha River
Total DNA was extracted through SDS or CTAB methods and DNA quality was checked via 2% agarose gel electrophoresis. The qualified DNA was diluted to 1 ng/L. The standard PCR assays were carried out using primers with barcode and the amplified PCR products were then pooled together with equal molar amounts to purify and recover. The library was constructed using Ion Plus Fragment Library Kit (Thermofisher, USA). After Qubit quantification and library detection, the library was sequenced on Ion S5TMXL sequencing platform (Thermofisher, USA). Beijing Novogene Co. Ltd (Beijing, China) was commissioned to carry out above experimental process and analysis.