ATAC-Seq of Bicyclus anynana: embryo at 3hrs
Eggs were collected between 2hr 45 min and 3hr 15min after oviposition. And samples were snap frozen immediately using liquid N2 and stored in -80C until further use. 15 embryos were pooled together for each replicate. ~80,000 nuclei were used for each library. Nuclei isolation and library preparation were done with little modification as described in Omni-ATAC protocol (Corces et al, 2017). Sequencing was performed at AIT Novogene, Singapore; Reads from the sample were aligned to the Bicyclus anynana (BaGv2) genome using bowtie. Peak call was done using F-seq.