GSM6267671: rDM_RepA; Bos taurus; RNA-Seq
mRNA was extracted using the Dynabeads® mRNA DIRECT™ Micro Kit (ThermoFischer). Subsequently 1ng of mRNA was reversed transcribed into cDNA using the Maxima H Minus Reverse Transcriptase (ThermoFisher) and the primers from the PCR-cDNA Barcoding kit (SQK-PCB109, Oxford Nanopore Technologies). Library prep including cDNA barcode was performed using the PCR-cDNA Barcoding kit (SQK-PCB109, Oxford Nanopore Technologies)