GSM5778152: B2; Homo sapiens; RNA-Seq
After 24h or 48 h of culture, NMK was treated with 1 ml of Trizol to isolate RNA for further bulk RNAseq experiments Libraries preparation for 150bp paired-end sequencing was processed using the TruSeq Stranded Total RNA Sample Prep Kit with Ribo-Zero H/M/R (Illumina Technologies., San Diego, CA, USA), following the protocol. The integrity and overall quality of the total RNA was quantified using a Nanodrop 8000 UV- Vis spectrometer (NanoDrop Technologies Inc., Wilmington, DE, USA) and checked with a BioAnalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). After cluster amplification of denatured templated, the prepared libraries were sequenced using Illumina Novaseq6000 (Illumina Technologies., San Diego, CA, USA).