small RNA-seq for pig: porcine alveolar macrophages
Small RNA Sample Pre Kit was used to construct the library. Special structures at the 3 'and 5' ends of Small RNA (complete phosphate groups at the 5 'end and hydroxyl groups at the 3' end) were used to start the Sample with total RNA.Add linkers at both ends of RNA, and then reverse transcription to synthesize cDNA. Then after PCR amplification, PAGE gel electrophoresis to separate the target DNA fragments, the cDNA library is recovered by cutting the gel.