ATAC-Seq of Bicyclus anynana: early pupal (3 to 6 hr after pupation) forewing
Forewings were dissected from both right and left sides of an individual from early pupa (between 3-6hr after pupation. 20 individuals) (40 wings) were dissected for each replicate. Samples were snap frozen immediately using liquid N2 and stored in -80C until further use. ~80,000 nuclei were used for each library. Nuclei isolation and library preparation were done with little modification as described in Omni-ATAC protocol (Corces et al, 2017). Sequencing was performed at AIT Novogene, Singapore; Reads from the sample were aligned to the Bicyclus anynana (BaGv2) genome using bowtie. Peak call was done using F-seq.