NextSeq 500 sequencing; The intercellular signaling network of ovarian cancer metastases
The intercellular signaling network of ovarian cancer metastases
Ascites or omentum was collected from patients with high-grade serous ovarian carcinoma undergoing primary surgery at the University Hospital in Marburg. Informed consent was obtained from all patients according to the protocols approved by the local ethical committee  RNA-extraction with TriFast (1ml) according the manufacturer protocol. RNA is dissolved in RNase-free water (with RNase-Inhibitor Superase-IN 1:20). DNase digestion with DNA-freeâ„¢ Kit (invitrogen, ThermoFisher scientific) followed. The integrity and purity of RNA was determined using the Experion RNA StdSens  Illumina TruSeq stranded mRNA according to manufactures' instructions