healthy implant screw hole 2
Total bacterial genomic DNA was extracted from host supragingival surface, implant surface or implant screw hole. PCR amplification of the hypervariable region of the 16S ribosomal RNA (rRNA) genes was performed using the universal bacterial primer pairs. Pairedend metagenomic sequencing was performed on the amplicon libraries using an Illumina MiSeq or HiSeq 2541 systems.