GSM6031746: ATAC-Seq T98G d9 CD45+HLA-DR+ rep3; Homo sapiens; ATAC-seq
5,000–10,000 cells from defined populations were separated via FACS and processed to prepare sequencing library as described previously (Buenrostro, Curr Protoc Mol Biol, 2015). Briefly, Sorted cells were centrifuged, washed, and resuspended in lysis buffer to extract nuclei. Nuclei were centrifuged and resuspended in Tn5 transposition reaction mix for 30 min at 37C. Transposed DNA was purified and stored at -20C. Libraries were constructed as described previously (Buenrostro, Curr Protoc Mol Biol, 2015). The quality was assessed using a High Sensitivity DNA Chip (Agilent Technologies), and library was sequenced using a NextSeq 500/550 High Output Kit (150 Cycles) on a NextSeq 500 (Illumina).