GSM5460899: MiRNA-101 overexpression 1; Homo sapiens; RNA-Seq
Total RNA was extracted using a mirVana miRNA Isolation Kit (Thermofisher Scientific) according to manufacturer's instructions. Transcriptome-wide mRNA sequencing was performed by the Australian Genome Research Facility (Melbourne, Australia) from four independent miRNA transfection experiments (n=4). RNA purity and integrity were confirmed by BioAnalyser (Agilent, CA). Libraries were prepared using the Ribo-zero Gold protocol (Illumina) and assessed by Bioanalyser DNA 1000 chip (Agilent).