mRNA-Seq of untreated bacteria
RNA integrity was checked by 2% gel electrophoresis, run at 100V for 1h, and checked by Agilent 5400 Fragment Analyzer System. RNA concentration was measured by NanoDrop, spectrophotometrically. RNA libraries were prepared using Illumina Stranded Total RNA Prep with Ribo-Zero Plus (Illumina, San Diego, CA, USA) kit. Sequencing was done by Illumina NovaSeq 6000 platform as paired-end (PE) 2x150bp layout. 4-5M reads per sample, about 1.5Gbp data were produced.