GSM5821743: SCIT2; Mus musculus; RNA-Seq
Total RNAs in each group were isolated and extracted using TRIzol reagents following the manufacturer's protocol.RNA integrity was evaluated using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Once the RNA were collected and integrity was confirmed, they would be prepared for cDNA amplification, library construction, and quality control. Once the quality of cDNA library was confirmed, the clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumia) according to the manufacturer's instructions (from Beijing Novel Bioinformatics Co., Ltd. (https://en.  novogene.com/)). After cluster generation, the library preparations were sequenced on an Illumina Novaseq platform and 150 bp paired-end reads were generated.