GSM6165976: HAP1 SETDB1 KO cells, small-RNA-seq; Homo sapiens; miRNA-Seq
Total RNA was resolved on a 15% denaturing polyacrylamide gel and a part of the gel containing 17-50 nt RNA was excised using a clean razor blade. Small RNAs were eluted from the gel piece by incubating in 250 μl of 1 M NaCl overnight at 4 °C and purified by ethanol precipitation method. The eluted RNAs were proceeded for Illumina sequencing library construction using NEBNext Small RNA Library Prep Kit for Illumina (New England Biolabs) and NEBNext Multiplex Oligos (New England Biolabs) according to the manufacturer's protocol.