GSM5822680: SHP77 (sRNA-Seq); Homo sapiens; miRNA-Seq
Total RNA was isolated from cells using the Magzol Reagent (Magen, China)according to the manufacturer's protocol Total RNAs were ligated with 3' RNA adapter, and followed by 5' adapter ligation. Subsequently, the adapter-ligated RNAs were subjected to RT-PCR and amplified with a low-cycle. Then the PCR products were size selected by PAGE gel according to instructions of NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Illumina, USA). Libraries were sequenced on the Illumina HiSeq 2500 with single-end 50bp following the manufacturer's protocols.