GSM4819901: LncMyoD_Input Rep 1; Mus musculus; OTHER
Lysates were clarified from sonicated nuclei and LncMyoD-DNA complexes were isolated with biotin-labeled DNA probes. Library was constructed using ChIRP-seq protocol from Chu et al., (2012). Briefly, LncMyoD-bound DNA was tagmented using TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme) and amplified to construct ChIP-seq library. Library was then sequenced using DNBSEQ-G400 sequencer (BGI) (2 x 100).