smallRNA-seq of male C. elegans; Input Rep3
NGS library prep was performed with NEXTflex Small RNA-Seq Kit V3 following Step A to Step G of Bioo Scientific`s standard protocol (V19.01). Libraries were prepared with a starting amount of 190 ng and amplified in 17 PCR cycles. Amplified libraries were purified by running an 8% TBE gel and size-selected for 144  163 nt. Libraries were profiled in a High Sensitivity DNA on a 2100 Bioanalyzer (Agilent technologies) and quantified using the Qubit dsDNA HS Assay Kit, in a Qubit 2.0 Fluorometer (Life technologies).All 6 samples were pooled in equimolar ratio and sequenced on a NextSeq 500/550 Flowcell, SR for 1x 84 cycles plus 7 cycles for the index read.