GSM5822682: DIPG4_DMSO treated [DMSO_2]; Homo sapiens; RNA-Seq
RNA was extracted using the RNeasy plus mini kit (Qiagen). Libraries were prepared using the Illumina TruSeq Stranded mRNA Library Preparation Kit; prepared libraries were quality controlled and quantified using a Qubit and Labchip Bioanalyzer. Libraries were pooled and run on a Illumina NovaSeq 6000 instrument Sequencing was performed by 2 × 150 bp paired-end read format.