Metatranscriptome of seawater sewater sample
RNA was purified from Sterivex units with Trizol (Invitrogen) and thenwe used the RNA Clean & Concentrator kit (Zymo Research, USA). Total RNA was  depleted of rRNA using RiboZero, and RNAseq libraries were prepared using NEXTflex Rapid Directional RNA-Seq Kit from Bio. Scientific.