GSM5196876: CON2; Homo sapiens; miRNA-Seq
Genomic DNA contamination of RNA samples was removed using RNase-free DNase Kit (Promega, Madison, WI, USA). The quantity and purity of total RNA were analyzed by Bioanalyzer 2100 (Agilent, Palo Alto, CA, USA). Approximately 1 µg total RNA without genomic DNA contamination was used for the sRNA library construction, according to the protocol of TruSeq Small RNA Sample Prep Kits (Illumina, San Diego, CA, USA). As a result, nine cucumber fruit sRNA libraries were constructed.