GSM5462004: MR_042_Mf_2_D100; Homo sapiens; OTHER
Monocyte-derived macrophages and dendritic cells were lysed upon collection with MagMAX™ Lysis/Binding Solution (Invitrogen, Thermo Fisher Scientific) and frozen in -80°C. RNA was extracted with KingFisher Flex automated extraction instrument (Thermo Scientific, Thermo Fisher Scientific Inc. Waltham, MA, USA) using MagMAX™-96 Total RNA Isolation Kit (Invitrogen, Thermo Fisher Scientific) following manufacturer's instructions. The RNA quality and quantity were analyzed with Agilent 4200 TapeStation System (Agilent, Santa Clara, CA, USA). Targeted RNA-seq library preparation was conducted with Tempo-Seq (BioSpyder) detector oligonucleotides designed to target all human genes (~23,000). Library preparation was conducted in 96 sample batches according to BioSpyder kit user manual. 