Whole genome re-sequencing of Oncorhynchus tshawytscha: fin tissue
DNA was normalized using picogreen fluorescence; fragmented with the NEBNext Ultra dsDNA Fragementase, pooled and filtered with Minelute purification. The NEBNext Ultra End Prep was used for fragment end repair and fragments between 400-500bp were selected using 25x AMPure beads. Fragments were amplified with NEBNext Ultra Q5 PCR, cleaned with AMPure beads, and quantified. Before sequencing, NEBNext Adaptors were added to identify individuals within the pool.