GSM5461043: Control Vehicle in vivo; Mus musculus; RNA-Seq
~5,000 cells were loaded into one channel of the Single Cell Chip A for each sample using the Single Cell 3' v3 single cell reagent kit (10X Genomics) for Gel bead Emulsion generation into the Chromium system. Following capture and lysis, we synthesized cDNA and amplified it for 14 cycles following the manufacturer's protocol (10X Genomics). 50 ng of the amplified cDNA were then used for each sample to construct Illumina sequencing libraries.  Sequencing was performed on the NextSeq550 Illumina sequencing platform following 10x Genomics instruction for reads generation; at least 50.000 reads per cell were produced.