16S rRNA gene amplicon of homo sapiens : adult fecal material
The V3-V4 region of 16S rRNA genes was amplified in duplicates for 25 cycles with DNA from faecal samples, following a two-step protocol using primers 341F-785R. After purification using the AMPure XP system (Beckman Coulter), sequencing was carried out with pooled samples spiked with 25% (v/v) PhiX standard library in paired-end modus (PE300) using a MiSeq system (Illumina, Inc.) according to the manufacturers instructions