GSM5582326: NT gRNA_rep1; Homo sapiens; RNA-Seq
RNA was isolated with the RNeasy MinElute Kit (Qiagen, #74204), treated with DNase I (NEB, #M0303S), incubated with an RNA strand reverse complementary to the antisense part of the respective guide RNA, heated to 95 °C for 3 min and purified again with the RNeasy MinElute Kit. Purified RNA was delivered to CeGaT (Germany) for poly(A)+ mRNA sequencing. The library was prepared from 200 ng RNA with the TruSeq Stranded mRNA Library Prep Kit (Illumina, USA)