Illumina NovaSeq 6000 paired end sequencing; Single-cell RNA-seq of Endometrial Superficial biopsies and full depth uterine tissue from organ donors
Single-cell RNA-seq of Endometrial Superficial biopsies and full depth uterine tissue from organ donors
Endometrial biopsies were obtained from live donors with written informed consent from all participants recruited from the Newcastle Upon Tyne Hospitals after ethical approval (reference 16/NE/0167, North East - Newcastle & North Tyneside 1 Research Ethics Committee). Endometrial biopsies were obtained using a disposable endometrial cell sampler, starting from the uterine fundus and moving downward to the internal cervical ostium. None of the subjects were on hormonal treatments for at least 3 months prior to the procedure. RNA was converted into cDNA and amplified as part of the 10x v3 chemistry Libraries were constructed using the 10x v3 chemistry