GSM5461266: SpG_ABE8e_BRCA1_tiling_MELJUSO_Dropout_RepB; Homo sapiens; OTHER
Cells were lysed and genomic DNA was extracted using? Genomic DNA was PCR amplified using barcoded primers, then pooled and SPRI purified before submission for sequencing. Oligonucleotide pools were synthesized by CustomArray. BsmBI recognition sites were appended to each sgRNA sequence  for cloning into the sgRNA expression plasmids, as well as primer sites to allow differential amplification of subsets from the same synthesis pool. The resulting amplicons were PCR-purified (Qiagen) and cloned into the library vector via Golden Gate cloning with Esp3I (Fisher Scientific) and T7 ligase (Epizyme)