sequencing protocol of synthetic template: M-17 (A-)
Custom sequencing protocol: 9 cycles of linear amplification with biotinylated oligos to add a first copy varietal tag; single round of linear amplification to make double-stranded fragments; purification of biotinylated fragments; 18 cycles of exponential PCR; standard library preparation steps (end-polishing, A-tailing, and adapter ligration); variable length library barcodes added. See manuscript for more details.