RNA-Seq Glaciar Sample
ACGGCATACGAGAT-[10 nucleotides barcode]-TACGGTAGCAGAGACTTGGTCT-3') of the Access Array Barcode Library for Illumina Sequencers (Fluidigm). The finally obtained amplicons were validated and quantified by Bioanalyzer and an equimolecular pool was purified using AMPure beads and titrated by quantitative PCR using the Kapa-SYBR FAST qPCR kit for LightCycler 480 and a reference standard for quantification. The pool of amplicons were denatured prior to be seeded on a flowcell at a density of 10pM, where clusters were formed and sequenced using a MiSeq Reagent Kit v3, in a 2x300 pair-end sequencing run on a MiSeq sequencer to obtain 100000 reads per sample aproximately. Genomic data were analyzed with Base Space platform.