ATAC-Seq of Bicyclus anynana: 5th instar T1-leg
T1-legs were dissected from late 5th instar larvae. 20 individuals (40 prolegs) were dissected for each replicate. Samples were snap frozen immediately using liquid N2 and stored in -80C until further use. ~80,000 nuclei were used for each library. Nuclei isolation and library preparation were done with little modification as described in Omni-ATAC protocol (Corces et al, 2017). Sequencing was performed at AIT Novogene, Singapore; Reads from the sample were aligned to the Bicyclus anynana (BaGv2) genome using bowtie. Peak call was done using F-seq.