GSM5268310: OSKM-ES_d25_1; Mus musculus; RNA-Seq
RNA was harvested using Trizol reagent (Thermo Fisher Scientific, 15596018). NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, E7530L)  was used for the construction of sequencing libraries. Poly-T-oligo-attached magnetic beads were used to isolate mRNA from total RNA, and first strand cDNA was obtained by reverse transcription PCR. The cDNA was then purified, end-paired, A-tailed, adapter-added and amplified by PCR.