GSM5583181: DE33NGSUKBR111905: THP-1 dual WT rep3; Homo sapiens; RNA-Seq
For isolation of total RNA, cells were resuspended in 350 µl RLT buffer (Qiagen) and then 350 µl 70 % (v/v) ethanol was added to the mixture. The samples were loaded onto Zymo Spin IIICG columns (Zymo Research) and washed sequentially with 350 µl buffer RW1 (Qiagen) and 350 µl Zymo RNA Wash buffer (Zymo Research). The RNA was eluted in RNase-free H2O. Total RNA was used to generate RNA sequencing libraries using the QuantSeq 3' mRNA-seq library prep kit FWD (Lexogen).