GSM6166341: Fam69c WT; Mus musculus; RNA-Seq
The cortex from seven male mice at 4 months of age were pooled according to genotype and dissociated using gentleMACS Octo Dissociator with Heaters (Mitenyi Biotec no. 130-096-427) and Adult Brain Dissociation kit (Miltenyi Biotec no. 130-107-677). Cells were resuspended in 0.5% BSA in PBS and filtered through 70 μm filter. Cells were stained with 7-AAD (Invitrogen, 00-6993) and sorted using the BD FACS Aria III (100 μm nozzle). Library construction was performed using Chromium Single Cell 3 Library and Gel Bead Kit v3.1 (10× Genomics, PN-1000121) with 13 or 12 indexing PCR cycles and sequencing on one high-output lane of the NovaSeq 6000 (Ilumina) with 28 bases for read 1, 150 bases for read 2 and 7or 8 bases for Index 1.