GSM4728141: JN-DSRCT-1_EWS-WT1_rep1_ChIPseq; Homo sapiens; ChIP-Seq
The chromatin was prepared using the Covaris TruChIP kit according to the manufacturer's protocol. Briefly, cells were fixed in 1% methanol-free paraformaldehyde for 5 min, quenched, and lysed. Nuclei were collected and sonicated on a Covaris M220 focused ultrasonicator. The final fragment size was determined using the TapeStation2200 (Agilent) and ranged from 100 to 300 bp. Equal concentration of crosslinked chromatin was immunoprecipitated Sequencing libraries were made from the purified DNA using the Kapa Hyper Library Preparation Kit (KAPA Biosystems)