GSM4878926: Main2.ADT.010819.l1; Mus musculus; OTHER
Spleens were removed from mice and mechanically dissociated with a syringe plunger over a 40 um strainer while being washed with 5 ml of PBS + 10% fetal calf serum. Suspensions were centrifuged briefly to pellet cells. Red blood cells were lysed with an RBC lysis buffer (155 mM NH4Cl, 12 mM NaHCO3, 0.1 mM EDTA) for 5 minutes and centrifuged again. 2 million cells from the resulting pellet were re-suspended in staining buffer (2% BSA, 0.01% Tween in PBS), and subsequently incubated with the antibody panel cDNA libraries were prepared following the standard CITE-seq and 10x protocols