GSM6031243: 312246716_FRA102220-002; Homo sapiens; RNA-Seq
Single cell encapsulation was performed using inDrop system from 1CellBio, followed by transfer to 1CellBio UV Cleavage Device to release oligo linkers from the hydrogel beads and RT reaction. For control sample cells were incapsulated and processed using 10x Genomics system, according to manufacturer's protocol. Sequencing was performed using the Illumina NextSeq 500 with the High Output Kit 75 cycles. Read format was as follows: Read1 50b, Read2 36b. Total cDNA reads output approximately 400Mb. For 10X Genomics, libraries were pooled and sequenced on NovaSeq 6000 and S4 lane with 28x150nt reads and produced over 5.4 billion reads.