RNA-Seq of A. trifoliata subsp. australis seeds during development
Total RNAs were extracted from A. trifoliata seeds during development. After removing genomic DNA using DNase I (Takara), mRNAs were obtained using olio (dT) beads and broken into short fragments, followed with cDNA synthesis. Paired-end sequencing was conducted on HiSeq X Ten platform (Illumina, CA, USA).