GSM5598574: BM_CD371P_GMP_rep2; Homo sapiens; RNA-Seq
The cDNA of treated 2000-cell aliquots were generated and amplified, as described previously(PMID:29434353, 31443434) Then qualities of the amplified cDNA were examined by qPCR analysis of housekeeping genes (B2m, Actb, Gapdh). Samples that passed quality control were used for subsequent sequencing library preparation, 1ng of dsDNA was used to generate the library using Vazyme TruePrepTM DNA library Prep Kit V2 for Illumina (TD-503). All libraries were sequenced by Illumina Novaseq6000 system (Novogene Co., Ltd. ).