GSM5461534: BMMs, Infection3; Mus musculus; RNA-Seq
Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0. The SMART-seq protocol was performed on single or pooled sorted cells following the manufacturer's instructions with some modifications. Briefly, the whole transcriptome was amplified with the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech).