GSM5582892: T. brucei passage 40A rep2; Trypanosoma brucei brucei; RNA-Seq
Trypanosomes were purified from whole blood by passage through a DE52 column, and RNA was harvested using Trizol reagent. Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (#E7530L, NEB, USA) with a total amount of 2 μg RNA per sample following the manufacturer's recommendations, and index codes were added to attribute sequences to each sample. RNA libraries were prepared for sequencing using standard Illumina protocols