GSM5417478: Sample 2 ME180/C4.15.1, CRISPR-Cas9 clone 1; Homo sapiens; RNA-Seq
RNA were extracted using respective Qiaigen isolation kits RNAs were isolated from control or siRNA transfected cells using a pure Link RNA mini kit from Ambion (12183018A; Life Technologies, Inc., Carlsbad, CA) and the libraries were prepared using the KAPA mRNA HyperPrep kit (Roche) according to the manufacturer recommendations. The final libraries were pooled at equimolar concentration and sequenced on an Illumina NovaSeq6000 sequencer (SP lane, 2x50bp).