GSM5351027: BCF; Bacillus amyloliquefaciens; RNA-Seq
The cultures were incubated at 30 ± 2 ℃ and shaken at 150 rpm for 24 h. Sample cells were harvested by centrifugation (10,000 g, 4 ℃), washed with sterile water, and then centrifuged and stored in liquid nitrogen immediately. A total amount of 3 µg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.