GSM4820797: Huh7_shControl_Rapa_ATAC-seq; Homo sapiens; ATAC-seq
Nuclei isolated from 50,000 counted cells were used for transposition reaction with transposase. Column-purified DNA was amplified in 20μl reactions with high-fidelity 2×PCR Master Mix using primers with unique barcodes to setup library. libraries were constructed following the NEB kit