GSM5290379: SBM3; Salmo salar; RNA-Seq
Total RNA was extracted using the RNeasy Mini Kit (Qiagen) following the supplier's instructions. Then, each total RNA sample was quantified using a NanoDrop TM 8000 spectrophotometer (Nanodrop Technologies). RNA integrity was determined using Agilent Bioanalyzer 2100. All samples showed a RIN value ≥ 8. Library preparation and RNA-sequencing were performed by the Norwegian Sequencing Center, using TruSeq Stranded mRNA library prep and Illumina HiSeq 4000 System (150 bp paired-end)