16S of V6: sewage water
Targets the V6 region of the bacterial 16S ribosome. Each sample is a composite of biomass collected from triplicate plates cultured under varying media and incubation temperatures with individual antibiotic amendments uniquely indexed for individual identification. After amplification, PCR amplicons from each sample was purified and quantitated and pooled at equimolar proportions into a single pool for sequencing. Each pool was loaded onto a single lane of a flow cell for cluster generation. The samples were run on a 125bp paired end sequencing run on the Illumina HiSeq2500.