intestinal microbiota of subadult European seabass
The bacterial DNA was extracted from whole intestine content of 20 experimentally fed subadult European seabass (4 replicates per treatment) using Invitrogen PureLink Microbiome DNA Purification kit (Carlsbad, CA, USA) following the manufacturers protocol. Commercial services of Microsynth AG (Balgach, Switzerland) were used for library preparation based on Nextera two-step PCR including purification and pooling. The V4 variable region of the 16S rRNA gene was successfully amplified from 18 DNA extracts using the MiSeq2000 Next Generation system (Illumina, San Diego, CA, USA).