Genome skimming: Astragalus wootonii var. wootonii - JLMC 9760
Fragmentation with Bioruptor Pico sonicator using 7 cycles of 15 s On/90 s Off; NEBNext Ultra II DNA Library Prep Kit with no size selection and half volumes, 6 PCR cycles using NEBNext Dual Index Primers i705 and i501; Index Read 1: ATTCAGAA, Index Read 2: AGGCTATA