miRNA-seq of sea cucumber:body wall
Raw reads were subjected to an in-house program, ACGT101-miR (LC Sciences, Houston, Texas, USA) to remove adapterdimers, junk, low complexity, common RNA families (rRNA, tRNA, snRNA, snoRNA) and repeats. Subsequently, uniquesequences with length in 18~26 nucleotide were mapped to specific species precursors in miRBase 22.0 by BLAST searchto identify known miRNAs and novel 3p- and 5p- derived miRNAs. Length variation at both 3 and 5 ends and one mismatchinside of the sequence were allowed in the alignment.