GSM5643614: OT-1 CD8_GEX; Mus musculus; RNA-Seq
OT-I CD45.1 CD8 T cells were sorted (CD45.1-PB, CD8 PerCP/Cy5.5, CD3 APC) from WT or DCDICAM-1 recipients 72h after immunization The libraries were prepared for sequencing according to 10x Genomics single-cell 3' v.1 1 (dual Index) with feature barcode technology for cell surface protein.The index of the hashing library was: NT-B8. Libraries were sequenced on one flow cell of a NovaSeq, with paired end reads as follows: read 1, 28 nt; read 2, 90 nt; index 1, 10 nt; index 2, 10 nt.