ovary 8h after spawning replicate 4
Extraction with the TruSeq Stranded mRNA Sample Preparation kit. The library validation has been made with Fragment Analyzer (Standard Sensitivity NGS kit) and qPCR (ROCHE Light Cycler 480).  Adapters are TruSeq Universal Adapter and TruSeq Adapter.  The library has been indexed  for sequencing of the 36 samples  on one flow cell with Illumina HiSeq 2500 with  SBS technology. (Sequence By Synthesis).