GSM5643589: wild type C57BL/6 #2; Mus musculus; Bisulfite-Seq
Viable leukemic cells were sorted on the BD FACSAria III. Genomic DNA was purified with the Qiagen Dneasy blood and tissue kit. 300 ng of DNA was fragmented to approximately 200 bp using a focal acoustic device (Covaris S2, Sage Sciences). Libraries were prepared with NEB-Next Ultra II Kit (NEB) according to the manufacturer's instructions low coverage wgs