GSM4820313: D543 MDDC LKO; Homo sapiens; RNA-Seq
RNA was harvested on Day 5 post-transduction using Trizol reagent.Illumina TruSeq stranded mRNA sequencing (100-PE w/base trimming after 150 PE ~40M reads 6Gb production target) was performed by Psomagen (Rockville,MD) was used with 500 ng of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols by Psomagen.