miR-Seq of human myoblasts
1) Extraction of total RNA; 2) ligation of RNA 3 adapter; 3) ligation of RNA 5 adapter; 4) RT-PCR to create single stranded cDNA; 5) PCR amplification using a common primer and a primer containing a unique index sequence for each sample; 6) gel purification of miRNA library; 7) validation of library quality; 8) quantification of library.