GSM5461977: Pool_Input_rep1; Crassostrea gigas; RIP-Seq
For each stage, 3 million embryos and 100 mg of pediveliger and spat were collected. Total RNA was extracted using Trizol reagent. m6A-RNAs were isolated with an antibody anti-m6A (ABE572, Millipore) followed by low/high salt buffer washing. The amount of 3.5 µl of IP and the equivalent amount of 50 ng of Input RNAs were used for the library construction according to the manufacturer's protocol without fragmentation option of the SMARTer Stranded Total RNA-Seq Kit v.2  (634418, Pico Input Mammalian). Paired-end 150-bp sequencing of Input and IP cDNAs of each sample were conducted on Illumina HiSeq 4000 platform at the Genome Quebec innovation Center (McGill University, Montréal, Canada).