GSM4822557: MMTV-PyMT tumor_PBS rep1; Mus musculus; RNA-Seq
Endothelial cells were sorted with FACS aria II (BD bioscience). RNA was extracted with Rneasy mini kit (Qiagen).  NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England BioLabs) was used for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols