GSM4728629: Manitou [S001CAD]; Triticum aestivum; RNA-Seq
Total RNA was extracted from leaf tissues using the Plant Mini kit (Qiagen) according to manufacturer's instructions and its quantity and quality were measured with the NanoDrop 2000. One μg of total RNA was used for cDNA synthesis using the QuantiTect Reverse Transcription kit (Qiagen). RNA-seq libraries were prepared according to Illumina's instructions, using the TruSeq RNA Sample Preparation Kit v2 (Illumina) and pair-end sequencing (126 cycles) was conducted on Illumina HiSeq 2500 following the manufacturer's protocols (https://www.illumina.com/documents/products/datasheets/datasheet_hiseq2500.pdf).