GSM4777753: ATAC-seq_A2iL-5; Mus musculus; ATAC-seq
Cells (500 to 3000 cells per reaction) were centrifuged to remove the supernatant. 20 μl of lysis buffer (10 mM Tris HCl (pH 7.4), 5 mM MgCl2, 10% DMF, 0.2% N-P40) was added, followed by pipetting 6-10 times to release the nuclei without purification, and 30 μl reaction buffer (10 mM Tris HCl (pH 7.4), 5 mM MgCl2, 10% DMF, 1000 unit Tn5) was mixed on a Vortex for 5 seconds. The reaction was incubated at 37 °C for 20 minutes, followed by addition of 350 μl ERC buffer (MiniElute, Qiagen) to stop the reaction. After DNA purification (MiniElute, Qiagen) and then performing ATAC-seq libraries construction. Sequencing libraries were generated using NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.