Genome skimming: Astragalus bicristatus - DSB 2722
Fragmentation with Bioruptor Pico sonicator using 7 cycles of 15 s On/90 s Off; NEBNext Ultra II DNA Library Prep Kit with no size selection and half volumes, 6 PCR cycles using NEBNext Dual Index Primers i708 and i507; Index Read 1: TAATGCGC, Index Read 2: ACGTCCTG