GSM5419207: anti-PD1-treated adult 4_PB_oW; Homo sapiens; RNA-Seq
Peripheral blood mononuclear cells were sorted by FACS and checked for purity. Then, RNA was isolated using Qiagen RNeasy Micro Plus kit (Cat#74034) according to manufacturer's instructions. Libraries were constructed using the TakaraBio SMART-Seq® HT Kit (Cat#634437) and indexing adapters added using Illumina Nextera XT (Cat#FC-131-1024) as per manufacturer's instructions.