mouse alveolar bone(scRNA-seq)
Eight 12-week-old male C57BL/6J wild-type mice (Chengdu Dossy Experimental Animals CO.LTD) were combined to extract single-cell suspensions. Mandible was carefully dissected under a stereo microscope to obtain the mandibular alveolar bone tissue. Soft tissues and molars are removed. Jaw bone around the incisor and behind condyle are cut off. Subsequently, the obtained alveolar bone tissue was cut into some pieces (<1mm3) and digested in 1 mg/mL collagenase type I (Worthington) and 1 mg/mL Dispase II (Sigma aldich) at 37C for 1 hour (200 rpm). The sample was then collected into a centrifuge tube through a 40 m filter, and the supernatant was removed after centrifugation. 1 mL of ACK lysis buffer was added on ice for 5 minutes to lyse red blood cells, and the supernatant was removed by centrifugation. The single cell suspension were prepared for scRNA-seq.