GSM4729221: ADT_UMT-derived CD8+ T cells; Mus musculus; RNA-Seq
CD8+ T cells were purified by autoMACS (Miltenyi Biotec; 130-096-543) and/or Mojosort system (MojoSort; 480008, 480044) according to the manufacturer's instructions. Peripheral CD8+ T cell populations derived from WT and B cell defective (µMT) strains each were labeled with oligoDNA-tagged antibodies against CD44 and CD62L (TotalSeq-A provided from BioLegend) and used for the single cell transcriptome analysis using 10x Genomics Chromium Controller. Sequencing libraries for gene expression were established by 3′ Library Construction Kit v3 (10X Genomics) and oligoDNA-antibody-tagged libraries were established by a procedure provided from BioLegend with custom primer sets. Expected target cell numbers and read depth are 3,000 cells each and 100,000 paired-end reads/cell. Gene expression-derived and antibody-derived libraries were pooled at the ratio of 9:1 for sequencing.