Genomic raw reads from Eristalis tenax (Longreads)
For DNA isolation E.tenax males were catched and stored in 99% molecular biology grade ethanol at -20C. 30-50 mg of thorax tissue were used in a single DNA isolation procedure. DNA was isolated making use of the Qiagen DNeasy blood and tissue kit, following the recipe for animal tissue. For long-read sequencing the DNeasy protocol was modified by replacing the steps where spin columns are used with magnet-based isolation steps, using 0.4 volumes of magnetic beads (AMPure). All other procedures, buffers and washing steps were done as described in the DNeasy (Qiagen) protocol for animal tissue samples. DNA quality and amount was checked with a Quantus Fluorometer (Promega). In addition integrity and size estimation of DNA fragments was evaluated with a fragment analyzer (Agilent). DNA isolation procedure as described above yielded 5  10 g total DNA. We used 2 g total DNA for library production with a ligation sequencing approach (Oxford Nanopore SQK LSK-108). Two sequencing runs with individual flowcells (R9) were run on the GridIon platform, each yielding an amount of long-read information between 7-9 Gbp.