Florida Snake Serpentovirus
Oral swabs were collected from host species, with RNA extracted using Zymo Quick-RNA MiniPrep Kit. RNA was concentrated using Zymo Clean RNA Clean and Concentrator kit (Zymo Research, Irvine, California). Samples were prepped by depleting ribosomal RNA using NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEBNext, Ipswich, Massachusetts) using manufacturer recommendations supplemented with AMPure XP beads (NEBNext, Ipswich, Massachusetts). Libraries were generated using NEBNext Ultra II RNA Library Prep kit (NEBNext, Ipswich, Massachusetts) using manufacturer protocols. Finally, prepped samples were pooled and loaded into an Illumina 600 cycle V3 MiSeq cartridge (Illumina Inc., San Diego, California) and run on an Illumina MiSeq system.