GSM5517759: Control transfection Replicate 2 (reanalysis); Gallus gallus; RNA-Seq
RNA was extracted (RNeasy Mini Kit) and reverse transcribed, and cDNA was amplified using a linear amplification system and used for sequencing library building (GATC). Random primed cDNA library, purification of poly-A containing mRNA molecules, mRNA fragmentation, random primed cDNA synthesis, adapter ligation and adapter specific PCR amplification, Illumina technology, 50,000,000 reads paired end with 2×50 bp read length.