GSM5352680: Lyz2-Cre/Myd88 fl/fl Untreated 1; Mus musculus; RNA-Seq
3 days post-RT (16 Gy), single cell suspensions from PK5L1940 tumors were generated. Tumors were manually minced using scissors, followed by a 45 minute enzymatic digestion in HBSS supplemented with 250 U/mL collagenase IV (Worthington) and 30 U/mL DNaseI (Roche), 5 mM CaCl2, and 5% FBS at 37o C with constant agitation. Immune cells from single cell suspensions were positively selected using anti-mouse CD45 MicroBeads (Miltenyi Biotec). Cells were then incubated in PBS containing Live/Dead Aqua, before a secondary incubation with anti-CD45. Live CD45+ cells were sorted using a BD FACSAria. Cells were processed according to the manufacturers protocol for the Chromium Single Cell 3' Reagent kit (v3.0) from 10X Genomics.