RNA-seq of Arachis glabrata
The PacBio SMRT sequencing library was constructed using equally mixed RNAs from different tissues. First-strand cDNA was synthesized using UMI base PCR cDNA Synthesis Kit (BGI). After synthesis of first strand, PCR amplification was performed to generate double-strand cDNA. Then, the cDNA was used for optional size selection for constructing a combined SMRTbell library. The library was subsequently sequenced using a PacBio sequel system.