GSM5049904: PVR-FM; Homo sapiens; RNA-Seq
FMs were cut by the vitrectomy cutter at 5000 cpm and the pellets were aspirated through the cutter tub into syringe. FMs were digested with collagenase type IV, followed by TrypLE™ Express Enzyme. The single-cell suspensions (1×10^5 cells/mL in PBS) were loaded onto microfluidic devices and scRNA-Seq libraries were constructed according to the Singleron GEXSCOPE protocol with GEXSCOPE Single-Cell RNA Library Kit (Singleron Biotechnologies, Jiangsu, China) (Dura B. et al.Nucleic acids research 2019). The individual library of each sample was diluted to 4 nM and pooled for sequencing on Illumina HiSeq X Ten platform.