GSM4780962: 2 MPI cells treated with 5 µg/cm^2 tremolite asbestos; Mus musculus; RNA-Seq
Cells were washed with PBS, lysed with the TRIzol™ reagent and stored at -80˚ C in RNAse/DNAse-free tubes. RNA was purified using RNeasy MiniKits (Qiagen, Mississauga, ON, Canada), quantified using Thermo Scientific™ Nanodrop™ ND-1000 and qualified by agarose gel electrophoresis. The mRNA was enriched by oligo(dT) magnetic beads. Illumina kits were used for the RNA-seq library preparation, which included procedures of RNA fragmentation, random hexamer primed first strand cDNA synthesis, dUTP based second strand cDNA synthesis, end-repairing, A-tailing, adaptor ligation and library PCR amplification.