PacBio HiFi sequence reads of T.pratense
Whole genome DNA was sheared on a Hydroshear to generate a fragment distribution peak near 23 kb. A BluePippin was used to select for fragments greater than 12 kb for library creation. Libraries were prepared using the SMRTbell express Template Prep Kit 2.0 and sequenced on a Sequel II. Reads were subjected to CCS correction and only reads with Qscores > Q20 were retained for fastq generation.