GSM5352398: RNA-seq_Nrd1-AA_+Rap_rep1; Saccharomyces cerevisiae; RNA-Seq
RNAs were extracted using Glass-beads and TRIzol (Invitrogen). For RT-qPCR experiments, RNAs were reverse-transcribed using SuperScriptII (Invitrogen). Primers used for reverse transcription and PCR are listed in Table S3. RNA library preparation and single-end stranded sequencing were performed at the iGE3 genomics platform of the University of Geneva. All RNA-seq experiments were performed in duplicates.