GSM5048993: IPSC_WT02; Homo sapiens; RNA-Seq
RNA was extracted using TRI Reagent and Direct–zol RNA microprep kit (both Zymo) including on-column DNase digestion to remove genomic DNA. Samples were quantified using an Agilent 2100 Bioanalyzer and passed QC. Forward stranded library preparation was done after ribosomal RNA depletion and sequencing with DNBseq platform sequencing (BGI), generating ~90 million paired-end 100 bp reads for each sample.