RNA-Seq of tabacco CMS and its maintainer
The mRNA was then enriched by using oligo(dT) coupled with magnetic beads. Using mRNA as a template, the first strand of cDNA was synthesised with random hexamers; the second strand of cDNA was synthesised by adding buffer, dNTPs, and DNA polymerase I;and the double-stranded cDNA was purified using AMpure XP beads. The purified double-stranded cDNA was subjected to end repair, a tail was added, and the sequencing connector was connected.cDNA libraries were obtained by polymerase chain reaction(PCR).