GSM5229444: Rev_4h; Homo sapiens; RNA-Seq
Organoids were harvested after 0 (mock), 4 and 16 hours post-infection were washed in cold PBS and incubated in TrypLE Express for 25 min at 37°C. When microscopic examination revealed that cells had reached a single cell state, they were resuspended in DMEM/F12 and spun at 500 xg for 5 mins. Supernatant was removed and the cell pellet was resuspended in PBS supplemented with 0.04% BSA and passed through a 40-μm cell strainer. For scRNAseq - Single-cell suspensions were loaded onto the 10x Chromium controller using the 10x Genomics Single Cell 3' Library Kit NextGem (10x Genomics) according to the manufacturer's instructions.