RNA-seq Smarcd1 wt-1 MPP
Total RNA from 1 X106 sorted Smarcd1-deficient or control MPPs or Pro-B cells was isolated using TrizolTM (Roche) and further purified using RNeasy columns (Qiagen). Sample quality was assessed using Bioanalyzer RNA Nano chips (Agilent). Transcriptome libraries were generated from 4 g total RNA using the TruSeq RNA Sample Prep Kit (v2) (Illumina) following the manufacturers protocols. Paired-end (2 x 100bp) sequencing was performed using an Illumina HiSeq2000 running TruSeq v3 chemistry. Cluster density was targeted at 600-800k clusters/mm2. Two transcriptomes were sequenced per lane