GSM5550741: Wild_Type_M0_BMDM_rep1; Mus musculus; RNA-Seq
Bone marrow cells were harvested from femurs and tibias of WT mice. Differentiation into BMDMs was treated with 20 ng/ml recombinant macrophage colony-stimulating factor (M-CSF, PeproTech) and maintained in culture for 7 days. 4 hours after treatment with IL-4 (M2-BMDM) or without IL-4 (M0-BMDM), cells were harvested for total RNA extraction using a Direct-zol RNA Microprep Kit (ZYMO RESEARCH). Polyadenylated mRNA was separated from 100 ng of total RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB). Sequencing libraries were generated using NEBNext Ultra RNA LibraryPrep kit for Illumina (New England Biolabs) according to manufacturer's instructions. Library concentrations were assessed by both a Bioanalyzer (Agilent) and NEBNext Library Quant Kit for Illumina (New England Biolabs).