GSM5856902: FA_2; Escherichia coli str. K-12 substr. MG1655; RNA-Seq
Total RNA extraction was done using the TRIzol-chloroform method. DNase treatment was done to remove any DNA contamination. Dnase treated RNA was treated with MEGAclear to remove small-RNA and produce high-quality RNA after which enrichment for mRNA was done using the MicrobExpress Kit. Paired-end, strand specific libraries for RNA sequencing were prepared using NEBNext Ultra Directional RNA library kit.The sequencing was carried out on HiSeq 4000  using 2x150 bp paired-end reads.