GSM5049421: p20136-s017_Day4Pool_Group2_BAL1_CITE; Macaca mulatta; RNA-Seq
Physiological saline, up to 50 ml was delivered through trachea to the lungs of anesthetized animals using a camera enabled fiberoptic bronchoscope. The flushed saline was re-aspirated 5 times before pulling out the bronchoscope. This collection was filtered through 70μm cell strainer and centrifuged at 2200 rpm for 5 minutes. Pelleted cells were suspended in 1ml R10 medium (RPMI(1X) and counted. BAL samples from 5 monkeys in each group were pooled together and two technical replicate 10X captures were performed. One replicate capture failed for the control group. The libraries were run on Nova Seq 6000 lanes and the resultant bcl files were converted to count matrices using Cell Ranger v3.1 CITE-seq-Count and scRNA-seq