Barcode sequencing of tumor-bearing lung: mouse transduced with sgMultiGEMM/Cre
Genomic DNA was isolated from bulk tumor-bearing lung tissue from each mouse. Three benchmark control cell lines (500,000 cells each) were added to each mouse lung sample prior to lysis to enable the calculation of the absolute number of neoplastic cells in each tumor from the number of sgID-BC reads. The sgID-BC region was PCR amplified from 32 g of genomic DNA using unique dual-indexed primers.