DNA-seq of avocado rhizosphere microbiome
The MoBio PowerSoil DNA Isolation Kit (QIAGEN, The Netherlands) was used to extract total DNA from rhizosphere soil samples (n = 10), following manufacturers instructions. DNA extraction products were sent to Macrogen Inc. (Seoul, Republic of Korea) for sequencing of the V1-V3 16S rRNA regions, using primers 27F (5-AGAGTTTGATCMTGGCTCAG-3) and 518R (5-GTATTACCGCGGCTGCTGG-3), in a 2250 bp paired-end run on a Illumina MiSeq platform.