GSM5006334: E423P-mitoRNA [mu1]; Drosophila melanogaster; RNA-Seq
Whole adult fly mitochondria were extracted and mitochondrial RNA was harvested using Trizol reagent. tRNAs were purified from total mitochondrial RNA and m1A&m3C demethylated, then partially  hydrolyzed according to Hydro-tRNAseq method. Fragments of ~19-35 nt for partially hydrolyzed and re-phosphorylated tRNAs  were converted to small RNA sequencing libraries using NEBNext® Multiplex  Small RNA Library Prep Set for Illumina® kit.