Genome sequencing of Methanomicrobium antiquum DSM 21220
The library construction was followed by the manufacture of SMRTbell Express template Prep Kit 2.0 (PB100-938-900). First, genomic DNA was sheared by Covaris g-TUBE (Part Number: 520079) and purified via AMPure PB beads (Part Number: PB100-265-900). After damage repairing and end repairing, the A-tailed inserts were ligated with T-tailed adaptors and removed small insert SMRTbell templates by performing size selection with the BluePippin System. The final SMRTbells were annealed with sequencing primers and bound to the proprietary polymerase using the Sequel Binding Kit v3.0 (Part Number PB100-626-600) to form the BindingComplex. After dilution, the library was loaded onto the instrument with Sequel Sequencing Kit 3.0 (Part Number PB101-597-900) and SMRT Cell 1M v3 Tray (PB101-531-000 or PB101-531-001) for sequencing.