RNA-Seq of L. biglobosa CAN1: isolates from canadian canola seeds
RNA-Seq library preparations were carried out from 1-2 g total RNA using the TransNGS stranded RNA-Seq Library Prep Kit for Illumina (TransGen, Beijing, China). The mRNAs were selected by oligo (dT) beads, chemically fragmented and reverse-transcripted to single-stranded cDNA using random hexamer primers. Next, the second strand was generated to form double-stranded cDNA. After that, the cDNA were end-repaired, adenylated, and adapter ligated. Finally, the ligation products were PCR-amplified to achieve the concentration required for sequencing. Each library was sequenced using 150 bp paired end reads chemistry on an Illumina HiSeq 2500 instrument.