sRNA-Seq of M.tuberculosis: H37Rv
Sample quality was assessed by Agilent RNA 6000 Pico Reagent on Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA) and quantified by Qubit RNA HS assay (ThermoFisher, Waltham, MA). Library preparation was performed with TruSeq smRNA Library Prep Kit (Illumina Inc., San Diego, CA) following manufacturers instructions. Average final library size is 150bp. Illumina 8-nt dual-indices were used for multiplexing. Samples were pooled and sequenced on HiSeq sequencer for 150 bp read length in paired-end mode, with an output of 20 million reads per sample