DNA-seq of complete chlproplastic genome in leaf: mature plant of P. victoria-mariae
DNA samples were randomly fragmented into ~400 bp using an ultrasonicator, followed by DNA library construction with an insertion fragment of 500 bp on an Illumina MiSeq 2000 (Illumina Inc, San Diego, CA, USA) platform by Shandong Huabo Genetic Engineering Co., Ltd (Jinan, China).  Paired-end sequencing was performed to obtain 150-bp sequences at both ends of each read.