GSM5049912: scRNA-seq of liver Lin-CD122+CD49a+ cells; Mus musculus; RNA-Seq
Beads with unique molecular identifier (UMI) and cell barcodes were loaded close to saturation. After exposure to cell lysis buffer, polyadenylated RNA molecules hybridized to the beads. Beads were retrieved into a single tube for reverse transcription. The library construction were performed according to the standard manufacturer's protocol (CG000206 Rev D).