GSM5229777: AB7_Subject 7 adenomyosis baseline [RNA_seq]; Homo sapiens; RNA-Seq
Total RNA was extracted from tissue stored in RNA later using RNeasy total RNA Kit (Qiagen, Hilden, Germany) as per the manufacturer protocol. cDNA library was constructed using well established Smart-seq2 protocol (Picelli et al. 2013, Picelli et al. 2014), tagmentation step was accomplished using Nextera XT kit (Illumina) , miRNA sequencing was performed as per the Hagemann et al 2018 protocol and sequencing was done in Illumina NextSeq 550® at the bioinformatics expression analysis facility at Karolinska University hospital, Sweden.