GSM5782247: F9721_36h_3; Zea mays; RNA-Seq
To prepare for the transcriptome sequencing, we performed a germination test of the two lines under the same conditions. For 72-3, germinating seeds at four-time points (0, 6, 12, 24 HAI) were sampled, while 6-time points (0, 6, 12, 24, 36, 48 HAI) of F9721 were sampled for RNA extraction. Three biological replicates were set for each time point of the two lines. Total RNA was extracted using RNA Easy Fast kit DP452 (TianGen BioTech Beijing Corporation). RNA purity, concentration, and integrity were determined using Nano-Drop TM 2000 spectrophotometer (Thermo Scientific, USA) Libraries of each RNA sample were constructed using Illumina Truseq RNA Sample Prep kits. The paired-end of 125 bp sequencing was performed with an Illumina HiSeq2500 sequencer