GSM5050373: H3K27Ac_H4; Mus musculus; ChIP-Seq
Ovary lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP was performed using a commercial kit (CST, 9003) Libraries were prepared for the ChIP DNA experiments with a TruSeq DNA LT/HT Sample Prep Kit (Illumina) and were subjected to quality control on a Bioanalyzer (Agilent 2200). Illumina sequencing was performed on an Illumina HiSeq 2000 Sequencing system at RiboBio Co., Ltd.