Amplicon (16S-V4) of Lake Sediment
Illumina high-throughput sequencing using a Nextera XT workflow and a 2x300 bp chemistry. The workflow utilizes transposome-based shearing which fragments the DNA and adds adapter sequences in one step. The DNA was amplified and dual-indexed with adapter sequences through PCR, using primers 515Ff and 806rB to target the V4 hypervariable region of bacterial 16S SSU rRNA gene sequences.