GSM5418417: TLR3; Mus musculus; RNA-Seq
TILs were isolated by digesting tumor fragments using Mylteni mouse tumor disassociation kit with enzyme R at 1:10 per manufacturer's guidelines. 1 to 5 × 106 cells were stained using fluorescently labeled mouse CD45 antibody (clone 30F11) and Fixable Viability Dye eFluor 506 (Thermo Fisher Scientific; Waltham, MA) to sort for live CD45+ and CD45− cells using the FACSAria II cell sorter. approximately 1.6x10^4 cells were onto Chromium Single Cell A chips and library construction performed using the Chromium Single Cell 5' Library and Gel Bead Kit protocol (10x Genomics) Libraries were sequenced using the NovoSeq 6000 platform (Illumina; San Diego, CA)