Illumina HiSeq 2000 paired end sequencing; Piperine Biosythesis in Black Pepper
Piperine Biosythesis in Black Pepper
Total flowers (spadices), individual berries (from spadices), leaves, and roots were collected from cuttings at different time points. Time after anthesis was recorded for each set of berries and berries as well as all other organs at the appropriate stage were randomly selected for triplicate measurements. Green house, 24°C (day) and 18ˆC (night) with supplemental illumination from R300 NS1-lamps (Valoya, Helsinki, FN) for 14 hours, Total irradiance at ~150-200 μmol/m2s-1. None Total RNA from frozen and bead mill macerated plant material was prepared by the Macherey-Nagel Nucleospin RNA Plant Kit (Düren, Germany) according to the manufacturer's instruction. RNA Total RNA was quantified by a Nanodrop UV-Vis spectrophotometer (Thermofischer, Dreieich, Germany) and quality controlled using a QIAxcel capillary electrophoresis system and software (Qiagen, Hilden, Germany) according to the manufacturer´s instructions. Library preparation using an unstranded protocol was performed by GATC Biotech (Konstanz, Germany).