GSM5551325: Hypo-D28_S5; Homo sapiens; RNA-Seq
Total RNA was purified using ISOGEN II (Nippon Gene, Tokyo, Japan) according to the manufacturer's protocol.  Purified total RNA was quantified using the NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific), and RNA integrity score was evaluated using the Bioanalyzer RNA 6000 Nano Assay Kit (Agilent Technologies, Santa Clara, CA, USA). RNA-Seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina   kit, NEBNext Poly(A) mRNA Magnetic Isolation Module, and NEBNext Multiplex Oligos for Illumina according to the manufacturer's instructions and subsequently validated for an average size of about 360 bp using  the Agilent DNA1000 kit.