GSM5418873: WT1_WENR; Mus musculus; ATAC-seq
50,000 live cells were sort purified and immediately resuspended in 50 µl of ATAC-Resuspension Buffer (0.1% NP40, 0.1% Tween-20, and 0.01% Digitonin) and incubated on ice for 3 minutes, and nuclei was spin pelleted and transposition was carried out for 30 minutes at 37C in a thermomixer DNA was purified using the DNA Clean and Concentrator (D4014, Zymo Research) and transposed fragments were amplified for 10 cycles using NEBNext 2x MasterMix and adaptors to prepare libraries. Libraries were cleaned and quantified using Bioanalyzer followed by sequencing.Adapters used to prepare sample libraries were design as mentioned in Buenrostro et al., 2013 Nature Methods (DOI: https://doi.org/10.1038/nmeth.2688). 