GSM4780422: WT_2_RNA; Mus musculus; RNA-Seq
Cerebella from various strains were isolated from 3-week-old mice and immediately frozen in liquid nitrogen. RNA-Seq libraries were prepared using the TruSeq v2 mRNA kit (Illumina). Briefly, total cerebellar RNA was isolated and RNA quality was assessed on Agilent TapeStation. mRNA was purified using biotin-tagged polydT oligonucleotides and streptavidin-coated magnetic beads. After fragmentation of mRNA, libraries were prepared according to manufacturer's instructions. Paired end reads (2x100bp) were obtained using the HiSeq 4000 (Illumina). Ribosome profiling libraries were generated as previously described (Ingolia et al., 2012; Ishimura et al., 2014) and sequenced on an Illumina HiSeq 4000.