ChIP-PCR on the pathway reporters in K562 TRIP clone 5 on sample: ChIP_bcPCR_clone_5_EV_Input_R3
ChIP-PCR on DSB K562 TRIP clone 5 for repair factors, the cells were transfected with sgRNA LBR2 or an empty vector, 8 hours later Cas9 was activated and 16 hours after activation the cells were collected and processed for ChIP. After standard ChIP procedure the samples were amplified by two successive PCRs on the integrated pathway reporters to generate the libraries, spanning the sgRNA target site and the barcode