RNA-Seq of Bommeria hispida: mature sporophyte
RNA extractions were made using the Sigma-Aldrich Spectrum Plant Total RNA extraction kit using protocol A. Approximately 20mg of plant tissue per extraction was used. Extracted RNA was stabilized and shipped in GenTegra-RNA (NBS Scientific, Canonsburg, Pennsylvania, USA) matrix. Sequencing was performed at the Duke Center for Genomic and Computational Biology (Durham, North Carolina, USA) using the KAPA (Roche) stranded mRNA-seq kit and all libraries were sequenced on the HiSeq 4000, 150bp paired-end