GSM5049551: 1_monthHFHI_4; Mus musculus; RNA-Seq
Brains were removed, cortex were dissected, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Illumina cat. no. RS-122-2301) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols - (https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedtotalrna/truseq-stranded-total-rna-sample-prep-ls-euc-ltf-15031060-e.pdf).