metagenomics of microbiome: human calves after swimming
linear amplification was applied with REPLI-g Mini Kit (QIAGEN) to enhance the amount of DNA while limiting the addition of bias. Sequencing libraries were made with Nextera DNA Sample Preparation Kit (Illumina) according to manufacturers instructions. Paired-end sequencing was done using MiSeqc (Illumina) and 150 bp length reads were generated for each end at MRDNA/Molecular Research Laboratory ( Shallowater, TX.).