GSM5050580: 0.8ug 4hr 3; Mus musculus; RNA-Seq
CD4+ T cells were sorted based on Nr4a3-Timer expression on a FACS Aria cell sorter and collected into eppendorfs. Cells were lysed in 100uL extraction buffer using PicoPure RNA extraction kit according to manufacturer's instructions. An on column DNase digest was performed. Libraries were constructed using Lexogen Quantseq 3' mRNA Forward kits according to the manufacturer's instructions using a starting amount of 15-20ng of RNA 3' mRNA-seq