GSM4824607: JMB81 in M199 rep 2; Candida parapsilosis; RNA-Seq
Total RNA was extracted from each and purified using RiboPure Yeast extraction kit (Ambion/Life Technologies). RNA integrity was assessed using agarose gels and Agilent Bioanalyzer. RNA-seq library preparation and sequencing of extracted total RNA was performed following ribosomal RNA depletion using paired end reads (Illumina HiSeq2000), 2x150 bp, from all samples.