GSM4780443: Untreated-KO Replicate 2; Mus musculus; RNA-Seq
Heart tissue dissected and immediately snap frozen in LN2. RNA isolated according to protocol: Omega Bio-Tek EZNA Total RNA Kit I (SKU: R6834-01) Total RNA quantified using the Invitrogen Qubit RNA HS Assay kit (Invitrogen, Carlsbad, CA) was serially diluted to masses characteristic of single- and low cell number RNA-Seq (1ng). The Clontech SMARTer v4 kit (Takara Bio USA, Inc., Mountain View, CA) was used for global preamplification. Illumina sequencing library was derived from the resultant cDNA using the Illumina Nextera XT DNA Library Prep Kit (Cat. # 15031942).