GSM4780531: 30 °C_rep1; Triticum aestivum; RNA-Seq
After collected, the ovarian granulosa cells were put in liquid nitrogen quickly, and preserved in low temperature environment. TRIzol (Invitrogen, Waltham, Massachusetts, USA) was used to isolate total RNA from granulosa cells following a manufacturer's protocol. Total RNA quantity was determined with Nanodrop 2000 (Thermo Scientific, Waltham, Massachusetts, USA) and quality was assessed by running an 1% agarose gel electrophoresis, stained with 4S Red Plus Nucleic Acid Stain (Sango, Shanghai, China). RNA libraries were prepared for sequencing using standard Illumina protocols