Illumina HiSeq 2500 paired end sequencing
WES libraries were prepared from 500 ng of DNA per sample using the SureSelect XT Target Enrichment for Illumina Paired-End Multiplexed Sequencing Library (Agilent Technologies)using the Agilent SureSelect XT Library Prep Kit. Target enrichment was carried out using the Agilent SureSelect XT Human All Exon v5 capture set, and sequencing with 100-base paired-end reads of targeted enrichment libraries was carried out on the HiSeq 2500 sequencer (chemistry v3, high-output mode).
exome enrichment SureSelect XT Human All Exon v5