colorectal cancer tumor from patient70
FL-cDNA library was constructed using SMART-Seqv4 Ultra Low Input RNA Kit (Takara Bio) to synthesize cDNA, but excepted oligo DNA. The library was applied for 1D2 sequencing according to manufacturer protocol with some modifications. We used the 1D2 sequencing kit (SQK-LSK308) and R9.5 flow cell (FLO-MIN107).