Illumina MiSeq paired end sequencing
16S sequencing of biofilm collected from endotracheal tube after extubation from COVID-19 patient
DNA was extracted following the protocol described in: Nelson, M. T., Pope, C. E., Marsh, R. L., Wolter, D. J., Weiss, E. J., Hager, K. R., ... & Hoffman, L. R. (2019). Human and extracellular DNA depletion for metagenomic analysis of complex clinical infection samples yields optimized viable microbiome profiles. Cell reports, 26(8), 2227-2240. Amplification of 16S V3-V4 region and sequencing was done according to the protocol for Illumina MiSeq 16S metagenomic sequencing: Illumina 2013. 16S Metagenomic Sequencing Library Preparation, Document: Part # 15044223 Rev. B.