18S rDNA amplification of environmental smaples: V4 primer
The primers TAReuk454FWD1 (5'-CCAGCA(G/C)C(C/T)GCGGTAATTCC-3') and TAReukREV3 (5'-ACTTTCGTTCTTGAT(C/T)(A/G)A-3') were used to target the V4 region of eukaryotic 18S rRNA gene (V4, ~420 bp)