HAE-UC24-13dpi (amplicon-seq)
RNA was reverse-transcribed using AMV (Promega, Madison, WI). The amplicon was amplified using the specific primers containing the adaptor sequences. The PCR products were seperated on 1.5% agarose and excised for purification. The purified DNA samples were quantified on a microplate reader (Synergy LX, BioTek, Winooski, VT), and 500 ng of each DNA sample (20 ng/l) was sent for PCR amplicon-seq (AMPLICON-EZ) at GENEWIZ, Inc. (South Plainfield, NJ).