GSM5418896: ctbp-1 neurons; Caenorhabditis elegans; RNA-Seq
Whole C. elegans animals were dissociated (washed, treated with SDS-DTT, washed, treated with pronase, washed with L-15-10, and filter for size) and living, GFP+, DAPI- neurons were FACS sorted out of the dissociated mixture. Libraries were prepared using Chromium Next GEM Single Cell 3' Kit v3.1 Libraries were prepared using Chromium Next GEM Single Cell 3' Kit v3.1 according to manufacturer's protocol