GSM5293214: RIG-I_RIP_NSUN2-KO_RSV-infected_IP_2; Homo sapiens; OTHER
After 12-hour RIG-I overexpression, cells were lysed in 200 uL lysis buffer and save 10 uL as "input". The remaining lysate was subjected to FLAG-beads pulldown. After washing, the purified RIG-I bound RNA was eluted from beads by TRIzol reagent. Both the "input" and "RIG-I bound RNA" were directly subjected to SMARTer® Stranded Total RNA-Seq Kit v2. RNA-Seq. All libraries were sequenced on Illumina NovaSeq 6000 with single-end 100 bp read length.