Illumina NovaSeq 6000 paired end sequencing; Comparison of different hashing methods on human PBMCs and primary mice tissues
Comparison of different hashing methods on human PBMCs and primary mice tissues
Commercial frozen PBMCs from 3 different healthy donors (SER-PBMC-F, tebu-bio) were used according to 10x Genomics sample preparation protocol “Fresh Frozen Human Peripheral Blood Mononuclear Cells for Single Cell RNA Sequencing” CG00039 Rev C.  cells were collected and processed according to 10x Genomics CG000390 Rev B protocol.  DNA libraries were generated according to 10x Genomics CG000390 Rev B protocol.