GSM5697060: 2018.06.11_14--2018.06.11_44; Homo sapiens; RNA-Seq
PDL cells were washed with cold PBS after gentle scrapping from the extracted premolar. Total RNA was extracted from PDL cells using TRIzol Reagent. The RNA was stored at −70 °C and measured at an optical density of 260 nm. The mixtures of total RNA were incubated with Oligo dT. Real-time PCR was performed for the selected upregulated and downregulated genes to verify RNA-seq results. The library was constructed, sequenced using an Illumina HiSeq2500 sequencer, and the data obtained through RNA-seq.