GSM4824377: P16 Control EC ATAC 1; Mus musculus; ATAC-seq
Following a 10min centrifugation at 4°C, nucleic extracts were resuspended in transposition buffer for 30 min at 37°C. Transposed DNA was amplified by PCR with Nextera primers (Buenrostro, 2013) to generate Dual-indexed libraries. Library quality control was carried out using the Bioanalyzer High-Sensitivity DNA analysis kit. Libraries were sequenced on the Illumina Nova-seq 6000 platform.