GSM5583748: S843; Homo sapiens; RNA-Seq
Resection or mucosal biopsies of the terminal ileum and CD4+ cells were isoloated for RNA-seq and RRBS total RNA was extracted from purified CD4+ cells using Exiqon's miRCURY RNA Isolation Kit. Illumina TruSeq un-stranded RNA library preparation protocol was used for RNA-seq library preparation. The pair end sequencing was carried out using the Illumina HiSeq 2000 sequencer at 50bps (3-4 samples per lane), which generated 42-113 million pair-end reads for each sample. Raw data was processed using MAPR-Seq pipeline