GSM5823706: WT Light Rep 3; Acinetobacter baumannii ATCC 17978; RNA-Seq
Three independent biological replicates per illumination and darkness were used for extraction using Zymo's Direct-zol RNA Miniprep Plus kit. 1 mL of culture were pelleted by centrifugation and then lysed using the TRIzol reagent for each sample. DNA contamination and RNA quality were checked before samples were sent to psomagen for library preparation and cDNA sequencing. Psomagen used the TruSeq stranded Total RNA kit with the Novaseq 6000 S4 Reagent Kit.  Htseq 0.10.0 was used for sequence analysis with NCBI NZ_CP012004 and NZ_CP012005.1 being used as reference sequences.