16S amplicon sequencing of Cliona varians marine sponge collected in Southern Caribbean Continental Seashore Specimen 5
Total metagenomic DNA was extracted from the assay with the defined consortium and amplified following the protocol at   https://earthmicrobiome.org/protocols-and-standards/16s/ with primers 515F GTGYCAGCMGCCGCGGTAA and  806R GGACTACNVGGGTWTCTAAT. Then purified PCR products were used for library preparation according to https://doi.org/10.1111/1462-2920.12366