GSM4880200: Raw rep1 [ATAC-seq]; Mus musculus; ATAC-seq
50,000 cells were collected and washed at 500g at 40C for 5 min. The cells were resuspended in lysis buffer and immediately spun at 500g at 40C for 10 min. The nuclei were subjected to transposase reaction for 30 min at 37oC; termination of the reaction and DNA purification were performed using a MinElute Kit (Qiagen). The purified DNA was amplified as described before with NEBNext High-Fidelity 2x PCR Master Mix (NEB). The PCR amplified product was cleaned with AMPure beads (Beckman). The quality of the libraries was assessed with the Bioanalyser High Sensitivity DNA kit (Agilent). Libraries were quantified using the Universal Library Quantification Kit for Illumina (Kapa Biosystems) on a StepOne Plus Real-Time PCR (Applied Biosystems). The libraries were sequenced at the Genomics Facility at MRC/LIMS of ICL using the Illumina HiSeq 2500 platform to obtain paired-end 100bp reads.