GSM5229909: 892_normal; Mus musculus; RNA-Seq
Lgr6-EGFP-Ires-CreERT2; R26loxP-stop-loxP-tdTom mice were administered a single dose of 20 μg, tamoxifen at P42 to activate Cre-recombination. Four weeks after denervation surgery, Lgr6Tom were stained with anti-CD49f-APC (1:300, Biolegend) and isolated from both denervated and control sides of the same mouse. RNA was extracted using RNeasy Mini Kit (Qiagen) following manufacturer's instructions. Libraries were prepared using NEBNext poly(A) mRNA magnetic isolation module (Cat# E7490S) followed by NEBNext Ultra Directional RNA library preparation kit from Illumina (Cat# E7420L). Library quality was confirmed using an Agilent BioAnalyzer 2100 (Agilent) and library concentration was obtained using NEBNext library quantification kit for Illumina (Cat# E7630L).