GSM5048664: WT Th17 rep1; Mus musculus; RNA-Seq
CD4+ T cells were sorted from lungs, pelleted, frozen at -80C. Total RNA was isolated with Trizol, with an extra chloroform extraction to remove residual phenol and addition of glyco-blue as a carrier to promote RNA precipitation. Directional RNA-seq libraries were prepared from 20ng total RNA per sample using the NEBNext Directional Ultra II RNA Library Prep Kit for Illumina (New England Biolabs), with initial polyA+ isolation, by the RNA Sequencing Core at Cornell University.