GSM5206780: SC161_IPAH_50year_female; Homo sapiens; RNA-Seq
Explanted lungs were finely diced and digested for 1 hour in a mix of collagenase A/DNAse I.  A single cell suspension was generated by filtering lungs through 70 micron filters and resuspending cells in PBS containing 0.04% BSA.  Cells were loaded into the 10X Genomics Chromium instrument per manufacturer's directions. Libraries were constructed according to 10X Genomics' Single Cell 3' Reagent Kits v1 and Single Cell 3' Reagent Kits v2 User Guide single cell RNA-seq