Bisulfite targeted sequencing of human rDNA in Down syndrome
Bisulfite-specific primers were used to amplify 5 regions within the rDNA locus (RiboProm_1_F:GTTTTYGTTGTGAGTTAGGTAGAGTTT; RiboProm_1_R: AAAAAAACRTCCCCAACCTCC; RiboProm_2_F:  GTGTTTTTGGGTTGATTAGAGG; RiboProm_2_R:  CATCCCAAAACCCAACCTCTCC; 18S1_F: TAGTYGTTTTTTAGGTTTTT; 18S1_R: CRTACATTTATCAAATCAAA; 18S2_F: TGATYGGGTTGGTTTTGATTTG; 18S2_R: TTACCTACCTAATTAATCCTACCAA; 28S_F: GTATTTAGTTTTAGATGGAGTTTATT; 28S_R: AACTAACCAAAATTCCCTCA).Forward and reverse primers were additioned at each 5 end with Nextera adapter sequences (TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG and GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG). PCR amplicons were pooled sample-wise and indexed using Illumina Nextera XT Index Set A.