16S rRNA gene amplicon sequencing of fish-mucus from a wounded fish
Library preparation and amplicon sequencing was performed at the University of Illinois at Chicago DNA Services Facility, USA. The 16S primer sets (CS1-341F and CS2-806R primers) were used to amplify the hypervariable V3-V4 region of bacterial 16S genes. The amplicon sequences was generated using Illumina MiSeq platform with the sequencing strategy of 300 bp paired-end employing V3 chemistry for 16S rRNA genes.