Oxford Direct RNA Sequencing of Pseudomonas phage ERWIb: failed reads fast5
Viral dsRNA was denatured, polyA tailed, selected for polyA tails, and concentrated. The RNA was then prepared for Oxford Nanopore Direct RNA sequencing. Guppy 3.2.6 was used for basecalling. Using a subset of 44000 "passed" reads, genome segments were assembled using Canu v1.9.