sequences of bacteria
Feces were collected from NOD mice aged 4 weeks before immunization.Total genomic DNA was extracted using DNA Extraction Kit following the manufacturers instructions.For bacterial diversity analysis, V3-V4 (or V4-V5) variable regions of 16S rRNA genes was amplified with universal primers 343 F and 798 R (or 515F and 907R for V4-V8 regions).