Whole genome resequencing of Zea mays subsp. mays inbred line C123
Tissue from the newest leaf of five seedlings was collected and immediately flash frozen in liquid nitrogen. DNA was extracted using a standard cetyltrimethyl ammonium bromide (CTAB) DNA extraction protocol (Saghai-Maroof et al., 1984), and treated with 25 uL of RNase at 39 for 30 minutes. Genomic DNA for each genotype was submitted to Novogene (Novogene Co., Ltd., Beijing, China) for standard Illumina library construction.