GSM5649946: ANKLE1_3day_rep2; Homo sapiens; RNA-Seq
Total RNA from sorted cells was extracted by Trizol reagent (Invitrogen) and purified with column-based Direct-zol RNA Microprep kit (Zymo Research). rRNA depletion was done using NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) and RNA was purified using Agencourt RNAClean XP Beads (New England Biolabs). Library was prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Libraries were measured by Qubit™ dsDNA HS Assay Kit (Thermo Fisher Scientific) and quality control was done using TapeStation (Agilent). Libraries were pooled together and sequenced in University of Virginia Genome Analysis and Technology Core using the Illumina HiSeq 2500 instrument. 