GSM5646457: Cd11b+F4/80+CD169+CX3CR1neg sorted cells - rep 2; Mus musculus; RNA-Seq
Bone marrow was isolated from the hind limbs (tibiae and femur) by lightly crushing with a mortar and pestle, followed by sorting for population of interest. Cells were sorted into Trizol and RNA isolated using manufacturer's protocol with isopropanol precipitation. cDNA libraries were prepared using NEXTflex mRNA-sequencing kit (BIOO Scientific) and sequenced using a NextSeq500 sequencer (Illumina)