GSM5352394: HS_3: Hidradenitis suppurativa_rep3; Homo sapiens; RNA-Seq
HS and normal skin samples were obtained from surgical excisions. Tissue was manually disrupted using scissors, and processed using Dispase II (2.4U/ml, Thermo Fisher Scientific) and Collagenase type II (2 mg/mL, Thermo Fisher Scientific) at 1:1 ratio overnight at 4°C with intermittent vigorous shaking for single-cell isolation. The suspension was filtered through a 70 μm cell strainer. Dead cells and red blood cells were removed with Lympholyte Cell Separation Media, Human (Cedarlane). 10X Single Cell 3' v3