RNA-Seq of cellular colture
Total RNA was extracted using the QIAamp Viral RNA mini kit (Qiagen). Zika complete genome were amplified with a set of 3 or 4 pair of primers and the SuperScript III one-step reverse transcription-PCR (RT-PCR) system with Platinum Taq polymerase High Fidelity (Invitrogen). Library was prepared by using the Nextera DNA XT Sample Preparation Kit (Illumina) and sequenced for 300bp paired-end on Illumina MiSeq with kit MiSeq reagent kit.