GSM4879723: WT mESC - RNA-seq rep1; Mus musculus; RNA-Seq
RNA was extracted from approximately 1 x 10^6 cells with the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. RNA was eluted from the columns with 50 μL RNase-free water and quantified using a Nanodrop. Library preparation was performed as previously described in, using unique indexes for each sample (ESCs TAAGGCGA and CGTACTAG, Rcor2 KO AGGCAGAA and TCCTGAGC) for multiplexing. Paired end sequencing was performed using 19 bp forward (used for de-multiplexing) and 72 bp reverse reads, with an Illumina NextSeq550 sequencing machine.