RNA-Seq of homo sapiens
500 ng of the total RNA was used to create RNA-seq libraries using TruSeq stranded mRNA library preparation kit. Libraries were checked for quality using an Agilent 2100 Bioanalyzer and quantified with DeNovix fluorometer. The sequence was subjected to cluster generation and paired-end 2x75 nucleotide read sequencing on an Illumina NextSeq 500 sequencer and quality check of the raw data files using FASTQC software.