miRNA-Seq of fruiting bodies from Agaricus bisporus
Small RNAs was extracted from powdered A. bisporus fruiting bodies using mirVanaTM miRNAs Isolation Kit (Ambion, Life Technologies) according to manufacturers instructions and quantified using a NanoDrop2000 (Thermofisher, Spain). Low molecular RNAs were isolated by 15% TBE-urea denaturing polyacrylamide gel electrophoresis (PAGE) and ligated to specific adaptors (AGATCGGAAGAGCACACGTCT) at 3ends. After reverse transcription, appropriate amplification and purification, the cDNA was submitted to NGS single read 1 x 50 that was carried out using an Illumina MiSeq 2000 (Illumina, Spain).