GSM5696628: K9m2_High-carbon_replicat2; Nannochloropsis oceanica; ChIP-Seq
N. oceanica cells (1-2 million cells · mL−1) from 400 mL culture were fixed under vacuum for 30 min with 1% formaldehyde (Sigma)at room temperature. After fixation, cells were incubated at room temperature for ten min under vacuum with 0.15 M glycine. Nuclei were isolated as described (Wei, Xu, 2018). All subsequent end repairing, A-tailing, adaptor ligation, and library amplification steps were done following a standard protocol (Illumina). The final library was sequenced on an Illumina HiSeq 2500 instrument with 1×150-bp reads.