GSM5649967: 4_BLM_sgRNF31_T0_R2; Homo sapiens; RNA-Seq
Cells were then lysed by adding 600 µl of buffer RLT (Qiagen, 79216) and their total RNA extracted using a RNeasy mini kit (Qiagen, 74106) according to the manufacturer's instructions. PolyA+ stranded RNA Libraries were generated using the TruSeq Stranded mRNA sample preparation kit (Illumina)