Illumina of Aphelinus atriplicis genome
Extracted genomic DNA from individual male using a DNeasy Blood and Tissue Kit, that we then whole-genome amplified the DNA with a REPLI-g Kit (Qiagen, Valencia, CA). Because Hymenoptera are haplo-diploid with males being hemizygous, using a single male ensured that this library would have no heterozygosity. Paired-end 2x150 nt library prepared and sequenced at the Delaware Biotechnology Institute, Newark, Delaware, using an Illumina HiSeq 2500 (Illumina, San Diego, CA).