HTG-miRNA EDGEseq: mus musculus: adult male serum derived sEVs
Functional DNA nuclease protection probes (NPPs) flanked by universal wing sequences are hybridized to target miRNAs. Universal DNA wingmen probes are hybridized to the wings to prevent S1 nuclease digestion. S1 nuclease is added to digest excess non-hybridized DNA probes and non-hybridized RNA, leaving only NPPs hybridized to RNA fully intact and able to be amplified/barcoded. Heat denaturation releases the protection probes from the DNA-RNA duplexes and released DNA protection probes are ready for amplification/barcoding, quantification, and sequencing.