RNA-Seq of Bombax ceiba flower petal tissues: dark-red-line at early stage
RNA was extracted using the method described in the Trizol kit (Invitrogen, Carlsbad, CA, USA). Agarose gel electrophoresis and Agilent 2100 bioanalyzer (Agilent Technologies, Palo Alto, CA, USA) were used to detect the quality and integrity of RNA. Eukaryotic mRNA is enriched by Oligo (dT) magnetic beads, fragmented by fragment buffer, and reverse transcribed into cDNA using random primers. The second strand is synthesized using DNA polymerase I, RNase H, dNTP and buffer. The cDNA fragments were purified using the QiaQuick PCR extraction kit (Qiagen, Venlo, The Netherlands), and poly(A) was added after the end repair, and ligated to the Illumina sequencing adapter. Use agarose gel electrophoresis to select the ligation products according to their size, and after PCR amplification, use the Illumina HiSeqTM 4000 sequencing platform of Guangzhou Gideo Biotechnology Co., Ltd. (Guangzhou, China) for sequencing.