Control
The adapter ligated DNA was purified with HighPrep Beads and then amplified for 15 cycles using primers provided in the NEXTflex Chip-Seq Library Prep kit. The final PCR product was purified with HighPrep Beads, followed by library quality control check. The sequencing library was initially quantified by Qubit fluorometer and its fragment size distribution was analyzed on Agilent TapeStation