GSM4824375: P16 Zeb1-CKO 1; Mus musculus; RNA-Seq
Total RNA pellets were dissolved in lysis solution and used as a template for low-cell number RNA-seq. cDNA was generated following the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech) protocol. The RNA-Seq libraries were sheared with a Covaris S220 and generated using Ovation Ultralow DR Multiplex System 9-16 (NuGEN Technologies). The libraries were sequenced using Illumina NextSeq 500 (2 x 150).