GSM5665772: DND41ResCas9_bioRep2_ctrl_Arima_HiC_rep1; Homo sapiens; Hi-C
1-2x10^6 cells were washed with 1xPBS containing 3% BSA and fixed with 2% formaldehyde and proceeded with Arima-HiC Kit per manufacturer's instructions. Samples passing Arima-QC1 were used for library construction with Accel-NGS 2S Plus DNA Library Kit (Swift cat# 21024) and 2S SET A INDEXING KIT (Swift cat# 26148) and assessed with Arima-QC2. Samples passing Arima-QC2 were subsequently PCR amplified based on Arima-QC2 calculations, quantified with TapeStation 4150 (Agilent) and KAPA Library Quantification Kit (Roche cat# KK4824) and paired-end (38bp+38bp) sequenced on Illumina NextSeq 550.