GSM6276616: PDA4a; Trichoderma asperelloides; RNA-Seq
Total RNA was extracted from homogenized tissue with TRI REAGENT (Molecular Research Center). Messenger RNA was purified using Dynabeads oligo(dT) magnetic separation (Invitrogen). Preparation of cDNA for sequencing followed the Illumina mRNA Sequencing Sample Preparation Guide. Complementary DNA was prepared using N6 primers for samples of all time-points. Sequencing libraries were produced from purified total RNA samples by the Illumina TruSeq stranded protocol. The libraries underwent 76 bp single-end sequencing using Illumina HiSeq 2500 in Rapid Run Model according to Illumina protocols.