GSM5583702: ZikV1; Homo sapiens; RNA-Seq
Cells were then directly lysed in TRIzol and RNA was extracted using the Zymogen RNA Clean and Concentrator Kit with on-column DNase I treatment. RNA quality was measured using the Agilent BioAnalyzer and quantified using the Qubit Fluorescent Quantification System (ThermoFisher Scientific). Libraries were prepared using the Illumina TruSeq Stranded Total RNA Kit with ribosomal RNA depletion. Paired-end sequencing with 150bp read-length was performed with the NextSeq 500.