GSM4824587: A_ctrl_120; Homo sapiens; RNA-Seq
Total RNA was extracted from at least 5x105 cells using RNeasy Plus Mini Kit (Qiagen, 74134) following the manufacturer's instructions and eluted in 10 ul of DNase/RNase free water. The concentration was determined using Qubit™ RNA HS Assay Kit (Invitrogen, Q32852) and the Qubit™ Fluorometer according to the company's protocol Extracted RNA samples were sent to the Barts and The London Genome Centre at Queen Mary University of London for processing. Library preps were made using ribosomal depletion.