GSM5006014: mutant_glucose_B; Vibrio cholerae; RNA-Seq
The entire culture was harvested (8,000 x g, 5 min, 4 °C) and the RNA was purified from the cells using the Direct-zol RNA Miniprep kit (Zymo Research) following the manufacturer's instructions. Eluted samples were treated with DNase at 37 °C for 30 minutes, twice, using Turbo DNA-free (Ambion), according to the manufacturer's protocol. RNA integrity was initially analyzed by agarose gel electrophoresis.