GSM5696884: H1581_R_rep2; Homo sapiens; RNA-Seq
Total RNA was extracted from the cells using RNeasy Plus Mini Kit, Qiagen (Hilden, Germany) with simultaneous DNase I digestion, according to the manufacturer's instructions. RNA purity and concentration were estimated with Nanodrop ND-2000 spectrophotometer, Thermo Fisher Scientific (Waltham, MA, USA). RNA quality was assessed using the 2100 Bioanalyzer with the RNA 6000 Nano Kit, Agilent Technologies (Santa Clara, CA, USA). All the samples had RNA integrity number (RIN) above 7.0. RNA libraries were prepared for sequencing using standard Illumina protocols