GSM5665779: DND41ResCas9_bioRep2_ctrl_RNAseq_rep3; Homo sapiens; RNA-Seq
5x10^5 cells were washed with 1 x PBS and lysed with 350 μl RLT Plus buffer (Qiagen) supplemented with 2-mercaptoethanol, vortexed briefly and immediately proceeded to RNA isolation with RNeasy Plus Micro Kit (Qiagen #74034). RNA integrity numbers were determined using TapeStation 4150 (Agilent), and all samples used for RNA-seq library preparation had RIN numbers greater than 9.5. 500-800 ng of total RNA was used, and libraries were prepared using the SMARTer Stranded Total RNA Sample Prep Kit-HI Mammalian (Clontech cat# 634873). Libraries were paired-end sequenced (38bp+38bp) on Illumina NextSeq 550.