GSM4824334: 11-b; Mus musculus; RNA-Seq
Synaptosome RNA was extracted via Trizol, synaptic RNA from microfluidic chambers was isolated with the GenElute™ Single Cell RNA Purification Kit after cutting the synaptic compartment out of the chamber Synaptosome small RNA libraries were done via NEBNext Small RNA Library Prep Kit, synaptosome toal RNA libraries via Illumina Truseq total RNA kit. Microfluidic total RNA libraries were prepared with Takara´s SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian, microfluidic chamber small RNA with Takara´s  SMARTer smRNA-Seq Kit for Illumina