RNA-Seq of mus musculus:mastocytoma
RNA was prepared from 0.5 x 106 undifferentiated and week 3 differentiated cells using the RNeasy kit (Qiagen) with RNase-Free DNase (Qiagen). MST cells grown in DME/F12 with 15% FBS were prepared in parallel for comparison. RNA sample quality was assessed by TapeStation (Agilent) with RNA Integrity Number greater than 8.0 deemed acceptable for generating sequencing libraries. The TruSeq Stranded mRNA Sample Prep Kit (Illumina) was used to generate sequencing libraries. RNA libraries were multiplexed and sequenced with 100 base pair paired end reads to a depth of approximately 50 million reads per sample on an Illumina HiSeq4000.