Altered innate immunity and damaged epithelial integrity in vaginal microbial dysbiosis
Sequencing of the V4 region of the bacterial 16S rRNA gene was performed using the 515FB-806RB primer set, per Earth Microbiome Protocols. Amplified in triplicate for each sample followed by pooling of each replicate. Pooled library was sequenced using a 300-cycle Illumina MiSeq Kit, generating 150bp paired-end reads.