ITS metabarcoding of macaw feces
Qiagen DNeasy Blood & Tissue Kit was used to extract DNA from green crab gut contents. The ITS gene region was the amplified from DNA extracts using fusion primers and two separate PCRs; sequencing was carried out with a 600 cycle Illumina MiSeq Reagent Kit v3 (2 300) and 5% PhiX spike in.