GSM5290991: WT REPLICATE 3 (total mRNA); Mus musculus; OTHER
RNA was extracted from bladder epithelial organoids. Total RNA was prepared using RNeasy Mini Kit with DNase I (Qiagen). RNA concentration and quality (A260/A280) was measured using a NanoDrop 1000 Spectrophotometer (Thermo Scientific). RNA libraries were prepared for sequencing using standard Illumina protocols.