GSM4824513: THP-1 co-cultured with MHCC-97H; Homo sapiens; RNA-Seq
Total RNA was prepared from THP-1 derived macrophages and THP-1 derived TAMs (THP-1 derived macrophages co-cultured with MHCC-97H or HepG2 cells) using the TRIzol reagent. The libraries were generated by using the Illumina TruseqTM RNA sample prep Kit (Illumina, USA). Then, the prepared libraries were sequenced using an Illumina HiSeq 4000 sequencer (Biomarker Technologies, China). The obtained sequence reads were filtered by using the SeqPrep and Sickle to delete some low quality reads. The final clean data was mapped to the human reference genome (GRCh38) using Tophat and gene expression changes were analyzed using Cufflinks.