Rattus_norvegicus_genome_male_muscle_3
We collected 25 mg of tissue and we purified DNA using the QIAamp Fast DNA Tissue Kit from QIAGEN (Cat. No. 51404) or the Zymo Quick-DNA Microprep Kit (Cat. No. D3024). For blood, we collected 150 l and we purified DNA using the Blood DNA Isolation Mini Kit from NORGEN BIOTEK CORP (Cat. No. 46300/ 46380). We verified the integrity of the DNA using 1% agarose gels. All DNA samples were tested for integrity (260/280 and 260/230 ratios >1.8), using a NanoDrop 2000 spectrophotometer (Thermo Scientific), and quantified in a Qubit 4 fluorometer (Thermo Scientific, MA, USA) with the Qubit dsDNA BR Assay kit from the same supplier. We selected 60 samples showing the highest LOY frequency based on the PCR screening, five for every tissue except for the kidney from which we selected ten samples. The 60 DNA samples were standardized to have 3 g of DNA in 30 l of TE buffer. Genomic DNA was sequenced at Novogene Facilities in California, USA. We generated on average 30 Gb of data per sample. Reads were paired-end and 150 nucleotides long.