GSM6245225: human urine-derived stem cells; Homo sapiens; miRNA-Seq
For exosomal miRNA sequencing, total RNA was extracted and purified from exosomes secreted by human USCs cells via an exoRNeasy Maxi Kit (Qiagen, Germany). 1.3 ug of total RNA was used for the construction of sequencing libraries. A total amount of 3 μg total RNA per sample was used as input material for the small RNA library. Sequencing libraries were generated using NEBNextRMultiplex Small RNA Library Prep Set for IlluminaR(NEB, USA.) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.