Rad-seq of Morpho helenor: adult male specimen
DNA was extracted from each sample from a slice of the thorax, using Qiagen kit DNeasy Blood & Tissue. DNA quantification (using microfluorimetric method) and quality controls (using electrophoresis and spectrophotometric method) were performed prior to sequencing. RAD library preparation and sequencing were performed at the MGX-Montpellier GenomiX platform (Montpellier, France). DNA was digested with the Pst1 enzyme and library was prepared according to Baird and Etter' protocol (2008) in a slightly modified version. Paired-end RAD-sequencing was performed on a 2 lanes flow cell of an Illumina HiSeq2500 in rapid mode so that reads (125bp) were expected to be of high quality with no missing base (N content).