GSM5597372: 10X_Circulation_Normal_3; Drosophila melanogaster; RNA-Seq
100 larvae were dissected for one single cell RNA seq library. Larvae were vortexed 1 min prior to dissection. 20 larvae were sacrificed in 10 ml of ice-cold Schneider's medium (Gibco, 21720024). Collected hemolymph was passed through a 40 mm cell strainer (Corning, 352340) and centrifuged at 7000 rpm at 4 °C for 5 min. After removal of the supernatant, 1X filtered PBS was added. After the cell preparation, scRNA-seq library generation was followed in 10X Genomics manufacturer's protocol (10X Chromium 3' v2 kit). Single Cell 3' Reagent Kit v2 (10x genomics)