GSM6246363: SB62_Placebo; Homo sapiens; RNA-Seq
Bone needle biopsy was centrifuged to remove loosely adherent marrow, and homogenized in QIAZol Reagent. Alternatively, a fraction of centrifuge bone was serially digested with liberase (2 digestions, 30 minutes, 37degreesC), washed and homogenized in QIAZol reagent. RNA was extracted from non-digested and digested samples, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser. RNA libraries were prepared according to the manufacturer's instructions for the NuGen Ovation RNA-Seq version 2 and Encore NGS Library Systems (San Carlos, CA).