GSM5418690: SW480 cells, D1KO_H3K4me1_ChIP-seq; Homo sapiens; ChIP-Seq
Lysates were clarified from sonicated nuclei and histone-DNA complexes were immunoprecipitated with histone H3 modification antibodies. ChIP DNAs were purified using PCR purification kit (QIAGEN). ChIP-seq libraries were prepared according to Illumina's instructions accompanying the TruSeq ChIP Library Preparation Kit (Part# 15023092 Rev. B).