GSM5293475: EMF1; Homo sapiens; RNA-Seq
RNA was extracted from whole cells by using Trizol protocol 500ng total RNA has been processed to obtain a library of indexed fragments by using Illumina Stranded mRNA kit (San Diego, CA, USA). Equimolar quantities of indexed library for each sample were loaded for sequencing on two flowcells Illumina MidOutput 150 cycles and sequenced by using Illumina NextSeq550dx (RUO mode).