GSM5911980: Father; Homo sapiens; RNA-Seq
PBMCs were filtered with 40 μm Flowmi Cell Strainer and counted. Viability was assessed (>90%) and cells were subjected to single cell isolation. Cell samples were loaded onto a 10x Chromium Single Cell Controller chip B (10x Genomics) for a targeted recovery of ~10,000 cells per condition, as described in the manufacturer's protocol (Chromium Single Cell 3' GEM, Library & Gel Bead Kit v3, PN-1000075). Generation of gel beads in emulsion (GEMs), barcoding, GEM-RT clean-up, cDNA amplification and library construction were all performed as recommended by the manufacturer.