GSM4879871: K562 Ctrl-1; Homo sapiens; RNA-Seq
Whole cell lysate was prepared by mechanical trituration in lysis buffer (20mM Tris pH 7.4, 150mM NaCl, 5mM MgCl2, 1mM DTT, 1% Triton X-100, 25U/ml TURBO Dnase) followed by RNA extraction using Zymo Research DIRECT-ZOL RNA Kit. Libraries were contructed using the TruSeq Stranded Total RNA kit from Illumina