GCS_MitoTMZ_repbio_1
Qiagen column RNA isolated from 5 x 105 GSC cells, treated with DNase (Qiagen79254). RNA controls with Tapestation (Agilent) with RIN > 8. Libraries constructed with TruSeq Stranded mRNA Library Prep kit (Illumina) with double indexing. RNA were reverse transcribed using SuperScript II (Invitrogen). Next generation sequencing was performed by 2x 100 bp paired end.