GSM5352722: hnRNPC_ADAR_D3_2; Homo sapiens; RNA-Seq
Cells were precipitated by centrifugation and either directly subjected to RNA extraction or separated into cytosolic vs nuclear/mitochondrial fractions as described in Baghirova et al, 2015 (PMID 26740924), using an increased digitonin concentration of 300 µg/mL. RNA extraction was performed using the Qiagen RNeasy Mini Kit including on-column DNase digest. Libraries were prepared using the TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold kit (Illumina).