GSM5396022: 3D_D35_1; Homo sapiens; RNA-Seq
Cells were recovered from the beads at days 10, 20 and 35 of differentiation, and total RNA was isolated using the Direct-zol RNA Miniprep Kit (#R2052, Zymo research). The targeted RNA-seq libraries were produced using the DriverMapT Human Genome-Wide Expression Profiling Kit, V2 (Cellecta, Inc.), that measures all 19,000 human protein-coding genes. All samples were sequenced in 38 paired-end reads on the Illumina NextSeq500 platform.