GSM4729696: Illumina_Undiff replicate 1; Mus musculus; RNA-Seq
RNA was extracted with an RNeasy Mini kit (Qiagen) 1 microgram of total RNA was processed using the Illumina TruSeq RNA Sample Preparation Kit v2 protocol and 700ng for the Illumina TruSeq Strand Specific total RNA with RiboZero Gold protocol (Illumina). Libraries were evaluated by Qubit and TapeStation. Sequencing libraries were constructed with barcodes to allow multiplexing of 9 samples on three lanes. Libraries were prepared using protocols RTA (1.17.21.3) and HCS (2.0.12.0).