survey library assembly of Dastarcus helophoroides: female pupae
For genomic survey, a paired-end library with insert size of 350 bp was constructed using the TruSeq Nano DNA Library Prep Kit (Illumina) following the Illumina protocols. After quality checking with Agilent 2,100 Bioanalyzer High Sensitivity Kit, the library sequencing was performed on an Illumina Novaseq6000 platform (Illumina, San Diego, CA, USA).