GSM5862457: hVCF-veh-557-cd4+; Homo sapiens; RNA-Seq
Total RNA was extracted from cardiac fibroblast cells using Qiagen RNeasy Mini Kit (Cat# 74134) isolation kit Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303) by Genewiz company. Initial sample QC analysis was performed followed by RNA library preparation with polyA selection and sequenced on illumina Hi Seq in 2 x 150 bp paired end configuration.