RNA-seq of Homo sapiens: uninfected monocytes: Sample U_RS102261_rna_mono_batch2_media
"CD14+ monocytes were isolated from cryopreserved PBMCs from tuberculosis resistant individuals (RSTR) and those with latent tuberculosis infection (LTBI). Monocytes were cultured in media alone or with Mycobacterium tuberculosis strain H37Rv (MOI 1) for 6 hours. Cells were Trizol-lysed and RNA extracted using Qiagen miRNeasy micro columns. cDNA libraries were prepared using Takara, SMARTer RNAseq Kit - Pico Input Mammalian."