genome resequencing of Raphanus sativus
Genomic DNA samples after testing qualified, with the method of mechanical interrupt (ultrasonic) DNA fragmentation, then end of fragmented DNA fragment purification, repair, and 3 'end and A sequencing, connection joints, and then selected fragment by agarose gel electrophoresis and sequencing library was formed by PCR amplification, build good library to library quality inspection, The qualified libraries were sequenced by Illumina.