Illumina HiSeq 4000 sequencing; 3' UTR RNA-sequencing of osimertinib vs. untreated Patient Derived Xenograft (PDX) models derived from EGFR-mutant lung tumors
3' UTR RNA-sequencing of osimertinib vs. untreated Patient Derived Xenograft (PDX) models derived from EGFR-mutant lung tumors
PDXs were implanted in the flanks of mice and treated p.o. as indicated. At the end of the treatment periods mice were sacrificed and tissues snap frozen. RNA was extracted using Qiagen RNeasy kits. Sequencing libraries were prepared using the QuantSeq 3' mRNA-Seq library kit (Lexogen, Austria)