16S_ITS_soil_WE_transect_forward_reverse
16S rDNA and ITS amplicons were amplified by a dual-barcoding preparation using protocols adapted from the Earth Microbiome Project. Pro341F/805R (V3-V4 regions) and ITS3_KYO2-F/ITS4-R (ITS2 region) primers  were designed in combination with heterogeneity spacers of 6 bp. Purified PCR products were sequenced  in Micromon Laboratory at Monash University (Victoria, Australia) in separate runs using a paired-end sequencing 2  300 bp.