16S metabarcoding of tomato rhizospheres
DNA from ~0.25g of rhizosphere soil was extracted with Qiagen DNeasy PowerSoil 96 HTP kits, according to manufacturer instructions. Samples were sent to the University of Minnesota Genomics Center for library preparation and sequencing of the V4  region of the 16S rRNA , according to the methods outlined in Gohl et al. (2016). Sequencing was performed on the Illumina MiSeq with V3 chemistry and paired end 300 bp sequencing.