RNA_seq of endophytic bacteria: Atractylodes lancea
Total bacterial community DNA was extracted with the PowerSoil DNA Isolation Kit (Mo Bio Laboratories, Solana Beach, CA, USA) from the rhizome tissues, rhizosphere soils and bulk soils was used for amplification and sequencing of the 16S rRNA, targeting the variable V5-V7 regions resulting in amplicons of approximately ~ 540 bp.An Illumina Miseq platform (TruSeqTM DNA Sample Prep Kit, Illumina USA) was used to detect diversity and composition of bacterial community.