PacBio HiFi sequencing data of high spine Gasterosteus aculeatus
High molecular weight DNA was extracted from the testes of a single Gasterosteus aculeatus using MagAttract HMW DNA Kit. Long-read sequencing was performed using Circular Consensus Sequence (CCS) mode on aPacBioSequelIIinstrument. Libraries were constructed with Express Kit v2 HiFi prep with SageELF size selection. Sequencing was performed using a 30 hour movie time with 2 hour pre-extension.