Illumina HiSeq 4000 sequencing; 3' UTR RNA-sequencing of human EGFR-mutant lung cancer cell lines under osimertinib treatment
3' UTR RNA-sequencing of human EGFR-mutant lung cancer cell lines under osimertinib treatment
Cells were treated with osimertinib for the indicated duration before being harvested by trypsination. RNA was extrcted using Qiagen RNeasy kits. Sequencing libraries were prepared using the QuantSeq 3' mRNA-Seq library kit (Lexogen, Austria)