WES of tail tissue from Ctds-deficient Tg(MMTV-PyMT) mouse
Genomic DNA was isolated from tumor pieces ( 10 mg) and tail lysates (germline control) of 18-week-old control and MMTV-cre;Ctsd-/- PyMT mice (4 each) using the QIAamp DNA Micro kit (Qiagen). DNA integrity was verified using a TapeStation system. The Agilent SureSelect XT Mouse AllExon Capture Kit was used for library preparation and afterwards sequenced on the Illumina HiSeq4000 platform with a read length of 100 base pairs (bp)