GSM4779306: Simvastatin2_rep4_ATACseq; Homo sapiens; ATAC-seq
hiPSC-ECs RNA was harvested using Trizol reagent. ATAC-seq DNA was extracted as described in previous protocol (Buenrostro J et al. Nat Methods 10: 1213-1218). Genomic DNA was clarified from sonicated nuclei and YAP-DNA complexes were isolated with antibody. RNA Libraries prepared from 10ng RNA, Sequencing data was un-stranded, ERCC spike-in (Ambion) used for data normalization.  ATAC-seq libraries were prepared for sequencing using TruSeq Library Prep Kit (Illumina). ChIP-seq libaries were prepared following Zymo Spin-ChIP kit protocol from Zymo Research.