Nanoplastics shape microbiome and functional metabolism
10 mL of each digested sludge sample was obtained for DNA extraction using a PowerSoil DNA Isolation Kit (MoBio Laboratories Inc., Carlsbad, USA). Partial fragments of the 16S rRNA gene were amplified using universal primer sets: 519F and 915R for the V4V5 region of archaea. The Illumina Nova 6000 platform was used to sequence 16S rRNA gene amplicons.