GSM5782193: PDGFb KO_  2wk post-MI; Mus musculus; RNA-Seq
Mice were euthanized and perfused with PBS supplemented with EDTA, and hearts were harvested. Infarcted tissue was dissected under a stereomicroscope.  The nuclei was released by homogenization with Pestle A and B (Sigma #D8938-1SET). Nuclei suspension was washed with PBS-0.01% BSA and filtered with series of 40 um strainers. Cells were treated and analyzed following a manufacturer 3' GEX library protocol (10x Genomics) and single-cell RNA-seq. single nuclei RNA-Seq