NextSeq 500 sequencing; smallRNA-seq of Solanum lycopersicum segregating for the sulfurea epiallele in cmt3 versus WT
smallRNA-seq of Solanum lycopersicum segregating for the sulfurea epiallele in cmt3 versus WT
100mg leaf tissue were flash frozen in liquid Nitrogen. Plants were germinated and grown in F2 compost and transferred to  16h light and 8h dark at 22C day temperature and 18C night, 70% humidity  Total RNA isolation was performed with the Direct-zol RNA Miniprep (Zymo Research) and Trizol reagent (Invitrogen) according to the manufacturerâ€™s instructions.  NEBNext multiplex small RNA library prep kit (NEB)