GSM5782078: hypostome 1; Hydra vulgaris; RNA-Seq
Pronase E was used to dissociaate tissue, Cells were washed through a 70 um then 40um cell strainer and resuspended in 1X PBS with BSA. single cell RNA-Seq libraries were constructed by following the Illumina Bio-Rad SureCell WTA 3' Library Prep Guide Libraries were multiplexed and sequenced as 43 bp paired end reads on a NextSeq 500 sequencer