NextRad: Anopheles darlingi genomic DNA
Genomic DNA was first fragmented with Nextera reagent (Illumina, Inc), which also ligates short adapter sequences to the ends of the fragments. The Nextera reaction was scaled for fragmenting 3 ng of genomic DNA. Fragmented DNA was then amplified for 25 cycles at 75 degrees, with one of the primers matching the adapter and extending 8 nucleotides into the genomic DNA with the selective sequence TGCAGGAG. Thus, only fragments starting with a sequence that can be hybridized by the selective sequence of the primer will be efficiently amplified. The nextRAD libraries were sequenced on a HiSeq 4000 with one lane of 150 bp reads