GSM5049245: Nuclear matrix rRNA-depleted RNA-seq; Homo sapiens; RNA-Seq
RNA from the lysates of different cellular fractionations  or total RNA was extracted by TRIZOL reagent. Libraries of the RNA-seq were prepared according to NEB's protocol of the NEBNext® Ultra™ II directional RNA library prep kits. Libraries of the SLAM-seq were prepared according to Lexogen's instructions of QuantSeq 3'mRNA-seq library prep kit REV for Illumina. Naropore libraries were prepared and sequenced according to Oxford's instructions for direct RNA sequencing. RNA-seq, SLAM-seq, Nanopore direct RNA-seq