GSM5049452: SH-EP Neuroblastoma cells - H3K27ac rep1; Homo sapiens; ChIP-Seq
Soluble chromatin lysates were derived from sonicated nuclei and histone-DNA complexes were isolated with antibodies against H3K27ac, H3K4me3, and H3K27me3. Purified ChIP DNA was used to prepare Illumina multiplexed sequencing libraries using the NEBNext Ultra II DNA Library Prep kit and the NEBNext Multiplex Oligos for Illumina (New England Biolabs) according to the manufacturer's protocol. Libraries with distinct indices were multiplexed and run together on the Illumina NextSeq 500 (SY-415-1001, Illumina) for 75 base pairs.