GSM5418803: PEER cell line - H3K4me3 - CRISPR-Cas9 editing; Homo sapiens; ChIP-Seq
10^7 cells  were incubated with 1% formaldehyde in RPMI medium for 10 minutes at room temperature, followed by addition of glycine to a final concentration of 0.125M. Cells were washed three times with cold PBS-BSA 0.5% before being pelleted and frozen at -80 ºC. ChIP-seq for the histone marks H3K4me3 and H3K27Ac (Diagenode) were included in this study.