Illumina NovaSeq 6000 paired end sequencing
Deposits were homogenised with a glass Dounce homogeniser. The lysate was then mixed in an iodixanol concentration gradient (25%-29%-35% concentration gradient, iodixanol Sigma-Aldrich, D1556) and centrifuged in a swinging bucket at 4,000 x g for 20 minutes. After centrifugation, 20,000 nuclei per sample were harvested from the nuclear band and treated with 100nM hyperactive transposase enzyme (Nextera Tagment DNA enzyme I #15027916) for 30 minutes at 37oC at 400 rpm, in an Eppendorf Thermomixer comfort incubator. The purified, transposed DNA was amplified using customised primers as per Buenrostro protocol