GSM5396227: 75C-FliC-RD5-D3; Homo sapiens; RNA-Seq
RNA was extracted using miRNeasy mini kit (Qiagen) Sample quality control was performed using the Agilent 2100 Bioanalyzer. Qualifying samples were then prepped following the standard protocol for the NEBnext Ultra ii Stranded mRNA (New England Biolabs). Sequencing was performed on the Illumina NextSeq2000 with Paired End 61bp × 61bp reads. Sequencing data was demultiplexed using Illumina's bcl2fastq2.