ISO-seq data of Idiosepius pygmaeus: CNS
RNA extraction: AllPrep DNA/RNA Mini Kit (Qiagen); Library prep: Iso-Seq Express Template Preparation for Sequel and Sequel II Systems protocol with standard size selection (86 L ProNex Beads); Sequencing: one SMRTcell of a PacBio Sequel II; Processing: Raw subreads from all four samples were compiled into circular consensus sequence (ccs) reads by ccs (v4.2.0) with minimum number of full passes set at three and the minimum predicted accuracy of a read at 0.9. Lima (v1.11.0) was used to classify the ccs reads as full-length (FL), remove index sequences with '--peek-guess' and demultiplex the reads. The resulting FL reads from each tissue/barcode are uploaded here.