GSM4823234: kDP turnover RP1140B all; Mus musculus; RNA-Seq
Thymi were prepared by gently smashing and passing through a 40uM filter to prepare single cell suspensions. DN3 and CD4 CD8 double-positive thymocytes were purified by FACS sort into Qiagen RLT lysis buffer. RNA was separated from genomic DNA using a modified oligo-dT bead-based method. Libraries were prepared following the Pico Nextera protocol.