Environmental DNA sequences collected from soil
DNA was extracted from 2g of dried soil using the PowerMax Soil DNA Isolation Kit. The 18S rRNA gene was amplified with primer pair Euk575F (5'-ASCYGYGGTAAYWCCAGC-3') and Euk895R (5'-TCHNHGNATTTCACCNCT-3') for identification of non-fungal eukaryotes, specifically soil animals. Both primers were equipped with unique 12-base Golay barcodes for multiplexing.