GSM5912295: RPF_DIMT1.rep2; Homo sapiens; OTHER
The cells were harvested by cell lifter after being washed with ice-cold PBS supplemented with CHX (100 μg/ml). The cell pellets were resuspended in lysis buffer (10 mM Tris-HCl at pH 7.4, 150 mM Kcl, 5 mM MgCl2, 100 mg/ml CHX, 0.5% Triton X-100, Protease Inhibitor Cocktail, Rnase inhibitor, and 100 μg/ml CHX) and kept on ice for 15 min. The cell lysates were centrifuged at 13,000 rpm for 15 min at 4°