GSM6245927: Mock 2w Rep 1; Homo sapiens; RNA-Seq
RNA was harvested using the RNA STAT-60 protocol. Total RNA was used for construction of sequencing libaries. RNA libraries for RNAseq were prepared with the NEBNextUltra directional library kit and the TruSeq stranded mRNA kit (Illumina). A 1.8pM library was spiked with 1% PhiX as an internal control and pair end sequenced with 101 x 51 cycles.