GSM5268571: 128189 R6 unst; Homo sapiens; RNA-Seq
After 4h cells were washed, and total RNA were isolated with the mirVana™ Isolation Kit (Thermo Fisher Scientific) according to the manufacturer's instructions. RNA-Seq library prep was performed with 100 ng total RNA input and the NEBNext Ultra RNA library prep protocol (New England Biolabs, Ipswich, MA, USA) according to standard procedures. Libraries were validated and quantified (Tape Station 4200, Agilent Technologies, Santa Clara, CA, USA). All libraries were quantified by using the KAPA Library Quantification Kit (VWR International, Radnor, PA, USA) and the 7900HT Sequence Detection System (Applied Biosystems, Foster City, CA, USA).