GSM5912304: A_ACY_2; Mus musculus; RNA-Seq
mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in NEBNext Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample. The library preparations were sequenced on an Illumina NextSeq 2000 platform and paired-end reads were generated.