GSM5462436: FMRP_cTag_NP_CLIP_4; Mus musculus; RIP-Seq
Microdissection was performed on hippocampal slices UV-crosslinked in HBSS with 0.1 mg/mL cycloheximide. Tissue was homogenized in lysis buffer (1x PBS, 0.1% SDS, 0.5% NP-40, 0.5% Sodium deoxycholate supplemented, 1X cOmplete Mini EDTA-free Protease Inhibitor (Roche) and 0.1 mg/mL cycloheximide) by passing through syringes with a 28 gauge needle. cTag FMRP-CLIP was performed as described previously (see associated paper for details). RNA tags were cloned using the BrdU-CLIP method as described in associated paper.