GSM6246155: C5ar1 WT neutrophils_D2 pi_Mouse 4; Mus musculus; RNA-Seq
RNA was isolated from the sorted cells using the Trizol reagent (Thermo Fisher) and further processed using a clean-up and concentration micro-elute kit (RNA Clean-Up and Concentration Micro-Elute kit, Norgen Cat# 61000), as per manufacturer's instructions. cDNA was amplified using the SMART-Seq v4 UltraLow Input RNA kit (Takara Bio, San Jose, CA) and sequencing libraries were prepared using the Nextera XT (Illumina, San Diego, CA). Multiplexed libraries were sequenced on a HiSeq3000 instrument (Illumina, San Diego, CA) in 50-bp single-end mode and mapped using STAR (v2.5.2).