GSM5395967: WGBS KO27_3; Arabidopsis thaliana; Bisulfite-Seq
Genomic DNA was extracted from 26-d-old plants grown under LD condition using the Qiagen Dneasy Plant Mini Kit. DNA fragments were treated twice with bisulfite using EZ DNA Methylation-GoldTM Kit (Zymo Research). The resulting single-stranded DNA fragments were PCR amplified using KAPA HiFi HotStart Uracil + ReadyMix (2x).