GSM5463669: JK2-78-14 RNA-Seq for CRC organoids; Homo sapiens; RNA-Seq
Organoids were collected from ~4-6 wells, and washed in 1x sterile PBS, and stored at -80°C as a cell pellet until both DNA and RNA isolation. All RNA samples were collected fromin triplicate biological replicates at baseline and resistance to EGFRi with persistent growth at 100% physiologic Cmax. An RNA library was constructed using TruSeq Stranded total RNA with rRNA reduction (Illumina). Quality control was performed by Eukaryote Total RNA electrophoresis using NanoDropOne. The library included 2x150bp paired end reads with sequencing was performed by NovaSeq 6000 using MiSeq NanoCell at UW Biotechnology Center.