GSM5462686: IL18Rαhi-2; Mus musculus; RNA-Seq
Total RNA was extracted from the isolated cells using Trizol reagent. cDNA libraries were prepared for 150 bp paired-end sequencing using TruSeq Stranded mRNA sample preparation kit. In brief, the mRNA molecules were purified and fragmented from 1 μg total RNA using oligo (dT) magnetic beads. The fragmented mRNAs were synthesized as single-stranded cDNAs using random hexamer primers. Double-stranded cDNA was prepared using this as a template for second strand synthesis. After sequential end repair, A-tailing, and adapter ligation, the cDNA libraries were amplified via PCR. The quality of these cDNA libraries was evaluated using the Agilent 2100 BioAnalyzer and they were quantified using the KAPA library quantification kit according to the manufacturer's protocol.