Illumina MiSeq sequencing of SAMD00294172
Viral RNA was extracted using the Quick Gene RNA cultured cell HS kit.  Total RNA was reverse-transcribed using reverse transcriptase with an oligonucleotide primer, which is complementary to the nucleotide sequences between position 1 to 20 on the negative-sense RNA of segment 5.  The cDNA derived from segment 5 was amplified by PCR using fusion primers, including the adaptors for the MiSeq sequencing system (Illumina, San Diego, CA): seg5 217 For overhang and seg5 465 Rev overhang corresponding to segment 5 between the nucleotide sequence positions 217 to 236 and 446 to 465, respectively