Illumina amplicon 16S rRNA sequencing of Brassicogethes aeneus abdomen
cDNA was synthesized from two pooled RNA extractions per species - one from dissected head tissue and one from abdomens. Samples were submitted for amplicon sequencing of the V4 hypervariable region of the 16S rRNA gene following the protocols of the Earth Microbiome Project. Library preparation and sequencing were performed at Argonne National Laboratory (Argonne, IL, USA), with paired-end 151 bp sequencing reactions on multiplexed samples on the Illumina MiSeq lanes.