GSM5229844: J3; Mus musculus; RNA-Seq
Tumors were digested with enzymes to obtain a cell suspension. ILC were cell sorted using a Aria3 cell sorter and defined as CD45+, lin- CD90+ and CD127+ cells.Fifteen thousand cells were captured thanks to Single cell 3' Gel Bead kit v3 and Single cell 3'GEM kit v3 on each well of a Chip B (10X Genomics). Library preparation, from reverse transcription to final amplification, was performed thanks to Single cell 3' library kit v3 (10X Genomics) by following manufacturer's instructions. Each library was 2*75bp paired-end sequenced on a NextSeq550 device (Illumina) at a depth of 200 million of reads, resulting in a read depth of 20000 reads per cell.