Nanopore DNA sequencing of Turicibacter bilis PIG517
Briefly, a 48 h old culture had total genomic DNA extracted with the PureLink genomic DNA extraction minikit (Life Technologies, Carlsbad, CA) and resultant DNA was assessed using gel electrophoresis, a NanoDrop spectrophotometer (ThermoFisher Scientific, Waltham, MA), and a Qubit 4 Fluorometer (double-stranded DNA broad-range kit; ThermoFisher Scientific, Waltham, MA). This genomic DNA preparation was used for sequencing using Nanopore sequencing technologies. An SQK-RBK004 rapid barcoding kit (Oxford Nanopore Technologies, Oxford, UK) was used to prepare a genomic DNA library for sequencing on a FLO-FLG001 (flongle) flow cell using a MinION instrument (Oxford Nanopore Technologies).