GSM5551493: RNA-Seq MGT_s2; Homo sapiens; RNA-Seq
On day 2, cells were collected and RNA was extracted using TRIzol. Cells were pooled from 16 wells per sample, and two biological replicates per condition were analyzed. Library preparation was performed by Novogene using their in-house preparation protocol. . Briefly, mRNA was enriched using oligo (dT) beads and fragmented randomly using a fragmentation buffer. cDNA was generated from mRNA template using a random hexamer primer followed by second-strand synthesis. Terminal repair, A ligation, and sequencing adaptor ligation were then performed. The final libraries were generated through size selection and PCR enrichment bulk RNA seq. Samples were sequenced to an approximate depth of 35–40 million reads per sample.