GSM5203717: GM23225_NPC_Supt4-kd; Homo sapiens; RNA-Seq
Total RNA was extracted using RNeasy kit (Qiagen) according to the manufacturer's instructions. ScriptSeq v2 RNA-seq kit (Epicenter) was used following the manufacturer's instructions. The workflow includes rRNA removal (RiboZero technology) followed by RNA fragmentation, ligation-free cDNA synthesis for preparing directional RNA-seq libraries. The final amplified libraries were further purified by gel excision and extraction. The libraries were analyzed using an Illumina HiSeq 2000 sequencer.