Hotspot_10_25_knockout_HiC
[1] K562 cells stably expressing Cas9 were infected with pgRNA for selected hotspot loci. The cells were collected for EGFP+ population at Day 9 and sorted cells were allowed to recover for 2hr before proceeding to Hi-C library. [2] 1 million cells were fixed with 2% Formaldehyde for 10 minutes at room temperature, followed by adding Stop Solution 1 (Arima-HiC kit) for 5 minutes. The fixed cells were wash with PBS before snap freezing for Hi-C library preparation. [3] Hi-C sample preparation was performed according to Arima-HiC kit (Arima Genomics Inc) as described by the manufacturer. [4] Hi-C library construction was performed according to Arima-HiC kit (Arima Genomics Inc) as described by the manufacturer.