Small RNA-Seq of Bos taurus: adult motile sperm
Small RNA libraries were prepared using NEXTflex Small RNA-Seq Kit v3 (PerkinElmer) according to the manufacturers instructions with the following approaches: adapters diluted 1:4, 3 ligation performed for 18h, samples amplified for 23 PCR cycles, then gel-free size selected according to optional step H1 in the instructions. Libraries were size selected to 140-170 bp using the SAGE Pippin Prep system