NextSeq 500 sequencing; RNA-seq of human multiple myeloma cell line JJN-3 virally transduced to overexpress PRL-3 compared to JJN-3 cells transduced with mock control
RNA-seq of human multiple myeloma cell line JJN-3 virally transduced to overexpress PRL-3 compared to JJN-3 cells transduced with mock control
Cell lines were pelleted by centrifugation (1500 RPM, 5 minutes) The JJN-3 cells were grown in RPMI 1640 + 10 percent FCS. Seeded in T75 flask, 20 ml medium. Split 1:10 or 1:15 every 3 to 4 days, when density of cells is normally between 0.3-0.7 x 10E6 cells/ml RNeasy Mini Kit (Qiagen, Crawley, United Kingdom) SENSE mRNA-Seq library prep kit (Lexogen GmbH, Vienna, Austria)