GSM5911646: VH1-2, mVHdel, VK1-33-CS, SP, IgK2; Mus musculus; OTHER
Splenic B cells were purified from 5-8 weeks old mice by MACS® Microbeads according to the manufacturer's protocol. In brief, spleens were dissected out from unimmunized mice, prepared into single cell suspensions and stained with anti-B220 Microbeads for 20 minutes at 4°C. Then, the splenic B cells were collected using the LS column and MACSTM Separater and lysed for genomic DNA extraction. 10ug of genomic DNA from purified splenic B cells was used for generating HTGTS-rep-seq libraries as previously described (Lin et al. 2016). 4 bait primers that target mouse JH1, JH2, JH3 and JH4 were mixed to capture all IgH heavy chain repertoire in one library. And 4 bait primers that target mouse Jk1, Jk2, Jk4 and Jk5 were mixed to capture all Igk light chain repertoire in one library.