GSM5583846: AR INPUT; Homo sapiens; ChIP-Seq
Chromatin was obtained from sonicated nuclei and AR-bound DNA fragments were isolated with antibody. Library was constructed for illumina sequencing using KAPA HyperPrep kit (Roche, #07962347001). Briefly, fragmented dsDNA was used for end repair and A-tailing. Illumina sequencing adapter was ligated to the end of DNA. Adapter-ligated library was PCR-amplified and purified using bead-based cleanup. Libraries were sequenced on the HiSeq 4000 to generate 50-bp paired end reads according to manufacturer's protocol.