GSM6244183: PDL1_antiPD1mAb_r2_donor_2_S10, Transcript portion of REAPSeq; Homo sapiens; OTHER
T cells were isolated using CD3 microbeads, resuspended at a concentration 1-2x10^7/mL in staining buffer plus 10% FBS. 50 uL of cells were incubated with 10 uL TruStain FcX Fc for 10 min at 4C. A lyophilized cocktail of 204 TotalSeqC antibodies were reconstituted in 50ul staining buffer. The antibody cocktail and 1 uL Total SeqC Streptavidin-PE were added to the samples and incubated for 30min at 4C, and washed 3x with 10 mL of cold staining buffer.  The cell pellets were resuspended at 1x10^6/mL in staining buffer prior to processing for sequencing. 10X Single Cell 5'V2_VDJ_Cell Surface Protein dual index protocol use poly-dT to capture 5' ends of the polyadenylated transcript in the final library