GSM5229512: dKO; Homo sapiens; RNA-Seq
Cells were processed using the 10X Genomics Chromium Controller with the Single Cell 3ʹ v3 Reagent Kit, according to manufacturer's directions. Briefly, a target number of 1250 cells per library was roughly achieved by loading 1.6 times the target number of cells in suspension. The Chromium Controller process each individual cells so that each cell is marked with its own unique barcode during reverse transcription. The end result is a single library representing one cell suspension, containing data for each individual cell.