GSM5696688: H3K27ac_cortex_06; Mus musculus; ChIP-Seq
Extracted chromatin was immunoselected with anti-H3K4me3 (#39159, Lot 22119006, Active Motif, Carlsbad, CA, USA), anti-H3K27ac (ab4729, Lot. GR3231887-1; Abcam, Cambridge, UK) and H3K27me3 (07-449, Lot. 3091919; Millipore, Temecula, CA, USA) antibodies following the procedure already described without limited reversal of crosslining (LRC) step. The bound fractions were decrosslinked, and purified DNA was used for library preparation. Libraries were prepared for sequencing using standard Illumina protocols