GSM4823303: B4886P1; Bos taurus; RNA-Seq
Subcutaneous AT (SCAT) samples were obtained from the right flank at 11 days before expected calving date and 6 days and 13 days after parturition, using the surgical procedure. Five grams of SCAT were collected, snap-frozen in liquid nitrogen, and stored at -80° C for RNA extraction. Total RNA was extracted from frozen samples using Trizol and the Quick RNA MiniPrep kit RNA libraries were prepared for sequencing using standard Illumina protocols