GSM5049136: eye disc control rep1; Drosophila melanogaster; RNA-Seq
Per replicate and genotype 40 eye antennal discs or 30 wing discs from third instar larvae were dissected. RNA was extracted via peqGold MicroSpin Total RNA Kit. Library preparation and RNA-Seq were carried out according to the NEBNext Ultra RNA Library Prep protocol, the Illumina HiSeq 1000 System User Guide, and the KAPA Library Quantification Kit - Illumina/ABI Prism User Guide