GSM5822825: hh_st3_rep2; Parasteatoda tepidariorum; ATAC-seq
Nuclei were extracted from 40 (for stage 3) or 200 (for stages 1 and 2) embryos in ATAC Lysis Buffer (10mM Tris, 10mM NaCl, 3mM MgCl2, adjusted to pH 7.4 with HCl) containing 0.15 mM Spermidine and 0.1% NP-40, followed by tagmentation with the Tn5 transposase. Transposed DNA was amplified with Illumina Nextera Transposase Adapters index PCR primers.