GSM5050307: Whole mesenchymal cells; Mus musculus; RNA-Seq
Intestinal mesenchymal were digested into single cells, the Epcam-CD45-fvd450- cells were sorted by FACS. for Lepr+cells,the GFP+ cells were sorted by FACS. Single cells were captured using the Chromium Platform (10X Genomics) and libraries were generated using Single Cell 3' v2 chemistry. Cell capture, library preparation, quality control, and sequencing was performed at beijing capitalBio technology Single Cell 3' v2 chemistry - 10X Genomics