WXS of canis lupus familiaris: matched normal DNA from whole blood
Pre-capture libraries were prepared with standard library preparation protocols using the KAPA Hyper kit from Kapa Biosystems and then pooled at equal volume and sequenced on the Illumina platform at low depth to determine exact relative abundances. Based on these abundances, libraries were balanced optimally for whole exome sequencing using the SureSelect Canine All Exon V2 bait set from Agilent.