GSM6245451: human, umbilical cord-derived, MSC, rep2; Homo sapiens; RNA-Seq
Total RNA was purified using an RNeasy Mini Kit (Qiagen, Valencia, CA, USA) 10 ng of total RNA were reverse transcribed  to obtain single-stranded cDNA using the SuperScript VILO cDNA Synthesis Kit (Thermo Fisher Scientific). cDNA libraries were  synthesised using the Ion AmpliSeq Transcriptome Human Gene Expression Core Panel (Thermo Fisher Scientific) and the Ion Ampliseq Library Kit Plus (Thermo Fisher Scientific), according to the manufacturer's protocol. Barcode-labeled cDNA libraries were analyzed using the Ion S5 XL System (Thermo Fisher Scientific) and the Ion 540 Chip Kit (Thermo Fisher Scientific).