GSM5463139: C2; Homo sapiens; ncRNA-Seq
Total RNA was isolated and purified using standard TRIzol Reagent (Invitrogen, Carlsbad, CA) according to the manual. RNA concentration and purity, RNA integrity were then assessed using the Nanodrop2000 spectrophotometer (Thermo Fisher Scientific, Wilmington, DE) and an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). The cDNA library was constructed using the Truseq RNA sample Prep Kit (Illumina, Inc., San Diego, CA). RNA sequencing (2*150 bp) was conducted on the Illumina HiSeq 4000 sequencing system (Illumina, Inc.). RNA libraries were prepared for sequencing using standard Illumina protocols