Illumina HiSeq 3000 paired end sequencing; The transcriptomic analysis of gene expression profiles associated with fungal keratitis in mice based on RNA-Seq
The transcriptomic analysis of gene expression profiles associated with fungal keratitis in mice based on RNA-Seq
Gene expression profiling analyses by high throughput sequencing were performed on mRNA samples isolated from PBS1、S2 and S5. C57BL/6 mice were routinely maintained in the Experimental Animal Room. RNA was harvested using Trizol reagent.TruSeq® RNA LT Sample Prep Kit v2  was used with total RNA for the construction of sequencing libraries. RNA was harvested using Trizol reagent.TruSeq® RNA LT Sample Prep Kit v2  was used with total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard BGISEQ500 protocols