GSM5049488: HFD-DEN-1; Mus musculus; RNA-Seq
Liver tissue was removed, wshed in ice-cold PBS, and  flash frozen using a liquid nitrogen-cooled biosqueezer. RNA was harvested using Trizol reagent. KAPA Stranded mRNA Sequencing Kit (Roche) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols