GSM5698662: Smad2 binding region in HaCaT cells stimulated with TGF-beta, replicate 1; Homo sapiens; ChIP-Seq
ChIP of HaCaT cells were performed using 10 μg of anti-Smad3 antibody (Isogaya et al, Cell Res 2011) or 10 ug of anti-Smad2 antibody (abcam, EP567Y), as described previously (Koinuma D et al, Mol cell biol, 2009). The libraries were constructed as described (Morikawa et al, Nuc Acids Res 2011). Adaptor-ligated samples were amplified by 15 cycles of PCR.