Microbiome of degrading fungus comb
The obtained Fast5 reads were basecalled using Albacore. The basecalled fast5 reads were then converted to fastq using Poretools. These fastq reads with filtered out on the basis of quality with the help of Nanofilt. Reads with quality score equal or more than 90% were chosen. These filtered reads were then used for removing adaptors and barcode using Porechop. Reads >= 150 and <=900 were selected and are submitted for generating sequence library.