GSM5516537: CRC00101; Homo sapiens; RNA-Seq
Peripheral blood mononuclear cells (PBMCs) were isolated following the standard procedure, and total RNA from PBMCs was extracted using TRIzol reagent A multiplex PCR amplification reaction was used to amplify the TCR immune repertoire. Human TCRα and TCRβ libraries were prepared using the HTAI-M and HTBI-M Kits (iRepertoire, Inc.) according to the manufacturer's instructions and sequenced on the Illumina MiSeq platform. Equal concentrations of each library with different barcodes were pooled and sequenced using the Illumina Paired-End Sequencing Kit (MiSeq, Illumina).