GSM5352292: 25dpf testis rep3; Danio rerio; RNA-Seq
Gonads were removed, and RNA was extracted using RNAprep pure Micro Kit (TIANGEN). For each sample, 5ul of RNA was used for synthesis and amplification of cDNA (Discover-sc WTA Kit,  N711, Vazyme), and 5ng of amplified cDNA was used for  construction of sequencing library using TruePrep DNA Library Prep Kit V2 for Illumina® (Vazyme TD501/TD502).