GSM5396318: CIN_CRC_scRNAseq; Mus musculus; RNA-Seq
Following enzymatic digestion, dead cells were removed using the EasySep Dead Cell Removal Kit (StemCell). CD45+ cells were purified using magnetic beads (StemCell) and then mixed at a 5:1 ratio of CD45+ cells to tumor cells. Libraries were constructed using the Single Cell Immune Profiling kit (10X Genomics).