GSM5049603: 470154_repl2; [Candida] auris; RNA-Seq
Total RNA was purified using Genejet RNA purification kit (Thermo Scientific). Quality of RNA was assessed on a Bioanalyzer using the RNA6000 Nanochip (Agilent), mRNA was enriched using oligo(dT) beads (NEB) and subsequently, double-stranded cDNA libraries were generated by using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB) according to the manufacturer's instructions. The qualified libraries were subjected to Illumina sequencing with a 75 bp paired-read length at the Novogene (Novogene, USA) sequencing facility.