GSM5823635: Input_2; Mus musculus; ChIP-Seq
For each biological repeat, 2×10^7 cells were cross-linked with 1 % formaldehyde at room temperature for 10 min and quenched with 0.125 M glycine. Samples were further sonicated and enzyme-sheared  with the ChIP-IT® Express Enzymatic Shearing Kit (#53035, Active Motif). Chromatin from 5×10^6 cells was incubated with 5µg antibody or IgG at 4 ℃ overnight, followed by purification of IP product with the Magna ChIP™ Protein A+G magnetic beads (#16-663, Millipore) , de-crosslinking, and extraction with the MinElute PCR purification kit (#28004, Qiagen). The libraries were constructed by Novogene Corporation (Beijing, China) using standard Illumina protocols