GSM5229703: Corticosterone RNA - 790_constant_rep3; Rattus norvegicus; RNA-Seq
RNA_Seq  RNA extraction  Frozen liver sections were homogenised (Polytron, PT-2100, Kinematica, CH) in TRIzol (ThermoFisher Scientific, USA). The aqueous phase was separated via chloroform extraction (Sigma-Aldritch, UK) and centrifugation (10,000 rpm, 40C), total RNA extracted, and DNA digested (miRNAeasy mini, Qiagen, US). RNA_Seq  Libraries were constructed from 1 µg total RNA (TrueSeq Stranded Total RNA kit, Illumina, USA) and filtered for a RIN score > 8 and < 10 (Agilent Tape Station, Bioanalyzer, UK) for sequencing (HiSeq 2500, Illumina UK).