GSM5822878: LPS_6h_3; Mus musculus; RNA-Seq
extracted from lung tissue using Trizol mRNA molecules were purified from total RNA using oligo(dT)-attached magnetic beads, mRNA molecules were fragmented into small pieces using fragmentation reagent after reaction  a certain period in proper temperture. First-strand cDNA was generated using random hexamer-primed reverse transcription,  followed by a second-strand cDNA synthesis.