NextSeq 550 sequencing; Decorin protects cardiac myocytes against simulated ischemia/reperfusion injury
Decorin protects cardiac myocytes against simulated ischemia/reperfusion injury
Cells were collected and lysed in 1 mL of QIAzol Lysis Reagent. (QIAgen) Primary cardiac myocytes were isolated from neonatal Wistar rats. Cells were cultured for 3 days prior to 4 hours of simulated ischemia/reperfusion injury. Cells were treated with 3nM decorin or it's vehicle (destilled water) for 20 hours before simulated ischemia. Total RNA was extracted using Direct-zol™ RNA MiniPrep System (Zymo Research) according to the manufacturer's protocol Gene Expression Profiling (GEx) library construction, QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen GmbH, Wien, Austria) was applied according to the manufacturer's protocol