GSM5550749: Wild Type replicate3 [WT_ColonMac_rep3]; Mus musculus; RNA-Seq
Single cell suspensions from colonic lamina propria were prepared, and colon macrophages were isolated using a FACSAria III Cell Sorter (BD Biosciences). Total RNA was extracted using a Direct-zol RNA Microprep Kit (ZYMO RESEARCH). Polyadenylated mRNA was separated from 20 ng of total RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB). Sequencing libraries were generated using NEBNext Ultra RNA LibraryPrep kit for Illumina (New England Biolabs) according to manufacturer's instructions. Library concentrations were assessed by both a Bioanalyzer (Agilent) and NEBNext Library Quant Kit for Illumina (New England Biolabs).