CASE1-60158612
ONT was performed on 1D genomic DNA sequencing for the GridION device using the SQK-LSK109 kit. The library was constructed from 1 g genomic DNA without frag-mentation and end repair. Adapters were ligated to both ends of genomic DNA. After purification on AMPure XP beads (Beckman Coulter Inc, Fullerton, CA, USA), the library was quantified by a Qubit assay with the high sensitivity kit (Life technologies, Carlsbad, CA, USA).