WGS-Resequencing of Ornithomya biloba: LX2018-16
After the DNA samples were tested and qualified, they were randomly interrupted using a Covaris ultrasonic fragmentation instrument, and then the whole library preparation was completed by end repair, addition of A-tail, addition of sequencing connectors, purification, and PCR amplification. After library construction, initial quantification was performed using Qubit 2.0 to dilute the library, followed by the detection of the insert fragment of the library using Agilent 2100, and the effective concentration of the library was accurately quantified using Q-PCR method after the insert fragment size met the expectation to ensure the quality of the library