GSM5552049: macrophage stimulated by RANKL and AnnexinA1 ANX_3; Homo sapiens; RNA-Seq
Total RNA was extracted using RNeasy Plus Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions, and the integrity of samples was assessed by determining 28S/18S ribosomal RNA bands with an Agilent 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). High-quality libraries assayed by Bioanalyzer High sensitivity DNA kit (Agilent) were subjected to Illumina NovaSeq 6000 System (Illumina).