18S rDNA amplification of environmental smaples: Amoeba_specific primer
Amoebal-specific primers Ami6F1 (5 CCAGCTCCAATAGCGTATATT 3) and Ami9R (5 GTTGAGTCGAATTAAGCCGC 3) were used to target the amoeba community (Amoeba-specific, ~600~700 bp)