GSM5516854: CD45+ leukocytes from ascites/peritoneal lavage (pooled from n=4 independent mice); Mus musculus; RNA-Seq
Target cell inputs of 10,000 cells were loaded into a Chromium Controller using Chromium Next GEM Single Cell 3' Reagent Kit v3.1 (10x Genomics) according to manufacturer's instructions. After generation of GEMs, cDNA synthesis and subsequent library preparation of all samples was completed using the Chromium Next GEM Single Cell 3' Reagent Kit v3.1 (10x Genomics) according to manufacturer's instructions. The 10X Libraries (Single Cell 3' v3) were sequenced on the Illumina NovaSeq6000 sequencer with pair-end reads (28 bp for read 1 and 90 bp for read 2).