GSM4880154: C11_9_CTRL; Mus musculus; RNA-Seq
Samples were isolated from lower incisors freshly dissected from newborn (PN0) wild type and K14cre;Nogo-A fl/fl pups. The dental epithelium was isolated by incubating the incisors in Dispase (2mg/ml in HBSS; D4818, Sigma Aldrich, Buchs, Switzerland) and immediately snap-frozen in liquid nitrogen. RNA was isolated using the RNeasy Plus Mini Kit (Qiagen AG, Switzerland) and subsequently purified by ethanol precipitation. The quality of the isolated RNA was determined with a Qubit® (1.0) Fluorometer (Life Technologies, California, USA) and a Bioanalyzer 2100 (Agilent, Waldbronn, Germany). Only those samples with a 260 nm/280 nm ratio between 1.8–2.1 and a 28S/18S ratio within 1.5–2 were further processed. TruSeq RNA stranded PolyA, TruSeq RNA Kit