GSM5293376: 029_JLat92_IFNB_8h; Homo sapiens; RNA-Seq
Cell lysates were digested in RLT and total cellular RNA extracted using the miRNeasy Micro kit (Qiagen) according to the manufacturer's instructions, and residual genomic DNA removed by DNAse treatment. mRNA-seq libraries were constructed using Illumina TruSeq® Stranded mRNA HT kit following the manufacturer's recommended protocol. Libraries were sequenced on an Illumina NextSeq500 sequencer using Illumina NextSeq 500/550 High Output v2 kits (150 cycles) following the manufacturer's protocol for sample handling and loading.