GSM4780607: AM24Hr2; Homo sapiens; RNA-Seq
Cells were collected and resuspended in 350uL of RLT Plus buffer and 0.01% 2-mercaptoethanol. Cells suspensions were homogenized by 29-gauge needle and QIAShredder Spin Columns. RNA was extracted with the Rneasy Plus Mini Kit following manufacturer's protocol with an additional on-column DNase treatment with an RNase-Free DNase kit. RNA was quantified and QC was checked using an Agilent 4200 TapeStation RNA ScreenTape. mRNA libraries were prepared using NEBNext Ultra RNA Library Prep Kit.