GSM5517749: Btn1a1-KO rep3; Mus musculus; RNA-Seq
Lactating mammary glands were removed, homogenised and flash frozen on dry ice in Trizol reagent (Zymo Research Corp). mRNA was fractionated from total RNA (2 mg/sample) using an Illumina TruSeq mRNA library preparation kit.  The mRNA was then fragmented and coverted to double-stranded cDNA and amplified by PCR to generate libraries for pair-end sequencing..