Ion Torrent S5 sequencing; Colletotrichum_H109_delimitation: raw reads
DNA was extracted from pure culture using the DNeasy Plant Mini Kit (Qiagen, Canada). The sequencing library was prepared using the Ion Torrent Ion Xpress Plus Fragment Library Kit (ThermoFisher Scientific, cat. no.: 4471269) and the Ion Plus Fragment Library Kit (ThermoFisher Scientific, cat. no.: 4471252). Genomic DNA was fragmented to 400 bp target size using the Covaris M220 Sonicator (D-Mark Biosciences Inc, part no.: 500295). Library fragment size selection was perfomed using the Pippin Prep (Sage Science, Beverly, Massachusetts, USA) with the 2% DF Marker L cassette (CDF2010) with the target size range set to 300-450 bp. Final library amplification was performed using 12 amplification cycles. 37 pM of sequencing library was loaded onto the S5 Chef instrument (ThermoFisher Scientific) using the Ion 530 kit (ThermoFisher Scientific) and sequenced on the Ion Torrent S5 sequencer (ThermoFisher Scientific).