GSM5290994: KO REPLICATE 3 (Subpolysome fraction); Mus musculus; OTHER
Organoid cell lysates were layered only 10-50% sucrose gradient and fractionated. Subpolysome fraction was collected. RNA was prepared using RNeasy Mini Kit with DNase I (Qiagen). RNA concentration and quality (A260/A280) was measured using a NanoDrop 1000 Spectrophotometer (Thermo Scientific). RNA libraries were prepared for sequencing using standard Illumina protocols.