GSM5897161: D2, Syngas, rep3; Clostridium autoethanogenum; RNA-Seq
Frozen pellets were thawed and total RNA was extracted using cellular lysis in RNase-free acid-washed glass beads (Merck) and RNeasy mini kit (Qiagen) with off-column DNase treatment followed by enrichment and purification using RNA Clean & ConcentratorTM (Zymo Research). Next, ribosomal RNA was removed using the Ribo-Zero rRNA removal kit (Illumina). mRNA libraries were prepared using standard TruSeq library preparation protocols (Illumina).