GSM6245753: MCF7 cells + E2   (E2 = b-estradiol) [WT_E2_1]; Homo sapiens; RNA-Seq
The cells were then harvested and total RNA was extracted using RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions. RNA quantity and purity was measured by a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific), and integrity was analyzed by an Agilent Bioanalyzer 2100 (Agilent Technologies). RNA-seq libraries were prepared using Illumina TruSeq Standard mRNA Sample Preparation Kit (Illumina) according to the manufacturer's protocol. Libraries were validated on an Agilent Bioanalyzer 2100. RNA-seq libraries were sequenced on a SE75 (single end 75 base pair) NextSeq500 flow cell.