RNA-seq yeast ZA1
Total RNA extraction was performed using the Direct-zol RNA MiniPrep kit (Zymo Research) according to the manufacturers specifications. Illumina sequencing libraries were prepared using 3-B and 5-A adaptors, as well as IGA-PCR-PE-F, TruSeq-MPEX-R, DSN-TruSeq-F, and DSNTruSeq-R primers.