non reactive
RNA extractions were performed using the RNeasy Plus Kit (Quiagen); RNA quality and concentration were assessed by a spectrophotometer and a Bioanalyzer (Agilent Technologies). Library construction was carried out using the NEBNext Ultra  RNA Library Prep Kit for Illumina. Libraries were generated through a paired-end 150bp (PE150) strategy using the Illumina Novaseq6000 sequencing system.