DNA-seq of the Drosophila melanogaster cell line mbn2 (Strand lab)
Cells were pelleted and DNA was extracted using the Qiagen Blood and Tissue kit. Purified DNA was analyzed for concentration and fragment size with Qubit and Fragment Analyzer, respectively. Library was prepared with the KAPA Hyper Prep Kit after acoustic DNA shearing with a Covaris E220 Evolution. Final library was multiplexed and sequenced on NextSeq500 high output flowcell with PE150 layout.