GSM4729691: Nanopore_ONTDirect Day4 replicate 2; Mus musculus; RNA-Seq
RNA was extracted with an RNeasy Mini kit (Qiagen). For cDNA-PCR library preparation, a total of 50 ng of poly(A) RNA was used as an input. Libraries were prepared according to manufacturer's protocols using the cDNA-PCR Sequencing kit (SQK-PCS108, ONT, Oxford, United Kingdom). For the Direct RNA library preparation kit (SQK-RNA002, ONT, Oxford, United Kingdom), an input of 500ng of poly(A) RNA was used. Libraries were prepared according to manufacturer's protocol.