GSM5551106: 293T-WT-rep1; Homo sapiens; RNA-Seq
Total RNA was extracted from 293T cells using TRIzolTM Reagent (#15596026, InvitrogenTM). Then mRNA was purified from total RNA using oligo(dT)-attached magnetic beads. First-strand cDNA was generated using random hexamer-primed reverse transcription, followed by a second-strand cDNA synthesis. The synthesized cDNA was subjected to end-repair and then was 3' adenylated. Adapters were ligated to the ends of these 3' adenylated cDNA fragments. The products were amplyfied, purified with Ampture XP Beads (AGENCOURT) and circularized by splint oligo sequence.The single strand circle DNA (ssCir DNA) were formatted as the final library.