RNA-Seq-Ctrl-3
Ribosomal RNA depleted RNA was isolated, purified by using RiboMinus Eukaryote Kit (Invitrogen, #A10837-08) and chemically shredded into ~100-nt fragments by using RNA fragmentation reagents (Invitrogen, #AM8740).  1 ug RNA of the fragments was used as the input (the rest for m6A-MeRIP-Seq analysis) and used to construct libraries with TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA). Sequencing was carried out on Illumina HiSeq 4000 according to the manufacturers instructions with single-end 50-bp read length.