GSM5352801: N1; Rattus norvegicus; RNA-Seq
The single-cell suspensions of DRG tissue were loaded onto microfluidic devices. Then, millions of beads with unique cell-barcodes and unique molecular identifiers (UMIs) were loaded similar to cells. Only one bead was loaded in each microwell. Following lysis, mRNA were captured onto the beads for the construction of sequencing libraries. single-cell RNA libraries were prepared for sequencing using standard Illumina protocols accompanying the GEXSCOPE Single-Cell RNA Library Kit (Singleron, China) single-cell RNA seq