scRNA-Seq of mus musculus: adult male eyes
P17 mouse retinal tissue were enzymatically dissociated as described above and 15 l of single-cell suspension at a concentration of ~900,000 cells/ml was loaded into one channel of the ChromiumTM Single Cell B Chip (10X Genomics1000073), aiming for a recovery of 8000-9000 cells. Cell populations were isolated from mouse retinal. Retinas of the eyes of two mice were taken at a time The Chromium Single Cell 3Library & Gel Bead Kit v3 (10X Genomics, 1000075) was used for single-cell barcoding, cDNA synthesis and library preparation, following manufacturers instructions according to the Single Cell 3Reagent Kits User Guide Version 3. Libraries were sequenced on Illumina novaseq 6000 using paired-end 150 bp.