Metabarcoding Bacteria: NTE_20
The 16S rRNA gene V3-V4 variable region PCR primers 341F (CCTACGGGNGGCWGCAG) / 785R (GACTACHVGGGTATCTAATCC) were used in a 30 cycle PCR. After amplification PCR products are pooled, purified and used to prepare an Illumina DNA library.