16S rDNA sequencing:Human gut microbiota
1. Microbial DNA was extracted using the HiPure Stool DNA Kit.  2. V3-V4 hypervariable region of 16s rRNA genes was amplified by PCR with the primers 341-F, 5-CCTACGGGNGGCWGCAG-3 and 806-R, 5-GGACTACHVGGGTATCTAAT-3. 3.After, amplification, the products were purified using the AxyPrep DNA Gel Extraction Kit and quantified using ABI StepOnePlus Real-Time PCR System. 4. Purified products were pooled in equimolar and paired-end sequenced (PE250) on an Illumina platform.