GSM6276592: BM, Male_Young_Old, Pool, GEX; Mus musculus; RNA-Seq
Bone marrow cells from young and old mice (5 mice in each group) were barcoded with TotalSeq hashtag antibodies (Table S1), pooled, and classical Ly6Chi monocytes (Ly6G-CD11b+CD115+Ly6Chi cells) were isolated by FACS sorting. Male and female mice were processed separately RNA libraries were prepared for sequencing using standard 10X protocols.