GSM5292841: Sample-19 (snRNA-seq); Homo sapiens; RNA-Seq
Single nucleus suspensions were isolated from about 50mg frozen human prefrontal cortex, homogenized in Nuclei EZ Lysis buffer (Sigma). Libraries were prepared using 10x genomics Chromium Single Cell RNA-seq platform (V3) For snRNA-seq and snATAC-seq, single nucleus suspensions were isolated from about 50mg frozen human prefrontal cortex following 10x genomics recommendation. For bulk RNA-seq, total RNA was isolated using Mini Nucleospin RNA kit, Takarabio Libraries were prepared using 10x genomics Chromium Single Cell ATAC platform (V1), 10x genomics chromium V3 for snRNA-seq and SMARTer Stranded Total RNA Sample Prep kit-HI Mammalian  for bulk RNA-seq