GSM4824539: Stroma_EnSC_biopsy 3; Homo sapiens; RNA-Seq
Total RNA was extracted from whole tissue, uNK, organoids, SUSD2+ CFUs (eMSC), SUSD2- CFUs (TA), SUSD2+ standard (PVC) and SUSD2- standard (EnSC) cultures using the AllPrep DNA/RNA Micro Kit (QIAGEN). RNA concentration was assessed using the Qubit RNA BR assay and RNA quality was analysed on an Agilent 2100 Bioanalyser (Agilent Technologies). Libraries were prepared using the TruSeq RNA Library preparation kit V2 and sequenced on HiSeq 4000 with 75 bp PE reads at the Wellcome Trust Centre for Human Genetics (Oxford, UK). RNA libraries were sequenced on two flow cells on the Illumina HiSeq 4000.