GSM5823274: Plusplus, NoTreatment; Mus musculus; RNA-Seq
For each sample, approximately 3000 cells were suspended in PBS with 0.04% BSA and loaded into the Chromium Controller, in which they were partitioned, their polyA RNAs captured and barcoded using Chromium Single Cell 3' GEM, Library & Gel Bead Kit v3 (10X Genomics). The cDNA were amplified and libraries compatible with Illumina sequencers were generated using Chromium Single Cell 3' GEM, Library & Gel Bead Kit v3 (10X Genomics). The libraries were sequenced on an Illumina NovaSeq sequencer on an SP100 cell flow (read1: 28 cycles; index: 8 cycles; read2: 80 cycles) at a depth of 50 000 reads per cell. scRNA-Seq