Sequence capture of Acrocephalus hiwae: USFWS2501-72913
Libraries were prepared using SureSelect XT kits with C-tailing and double indexed with iNext adapters (Faircloth & Glenn, 2012). Targeted sequence capture was performed on pooled libraries using a custom-designed Acrocephalus specific sequence capture kit (some samples underwent a separate sequence capture targeting the mitogenome using a pre-designed myBaits mito capture kit). Capture pools were pooled and sequenced using 150 bp paired-end on HiSeq 2500 (Brigham Young University).