GSM5552151: 6-COC; Homo sapiens; RNA-Seq
Conjunctival tissues were removed, conserved in RNALater Stabilization Solution (Qiagen) and frozen at -80oC. RNA was isolated from individual tissue samples using GeneJET RNA Purification Kit (Thermo Fisher Scientific) following manufacturer's instructions and using PureLink® DNase Set (Invitrogen, Carlsbad, CA, USA) to remove DNA contamination from columns. Illumina Truseq Stranded mRNA Sample Preparation Kit (catalogue #RS-122-2103, Illumina) was used with 0.5 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols