GSM4729717: Select Strain 65dph Biological Rep3; Oncorhynchus mykiss; RNA-Seq
Whole intestines were isolated from trout fry and stored in 1 mL of Qiazol (Qiagen) at -80C. Samples were thawed and homogenized on a TissueLyser (Qiagen). Total RNA was purified using a RNeasy 96 kit (Qiagen) with on-column DNase I treatment. For each sample, 800 ng of input RNA was used to isolate mRNA using the NEBNext Poly(A) Magnetic mRNA Isolation module (NEB; Ipswich, MA, Cat# E7490). The mRNA template was used to generate stranded, unique dual indexed libraries with the Ultra II Direction RNA Library Prep Kit (NEB, Cat#E7760). Sequencing was done on an Illumina NextSeq500 using a 150-cycle (2 x 75) v2.5 HIgh Output kit.