RNA Sequencing of LK638S-1_L
Total RNA was extracted from rice panicles and leaf using Trizol reagent (Invitrogen, CA, USA) and purified using a RNeasy Plant Mini Kit (Qiagen, CA, USA) according to the manufacturer's instructions. RNA degradation and contamination were checked by 1% agarose gel electrophoresis. The quality and integrity of RNA were assessed using an Agilent Bioanalyzer 2100 system (Agilent, CA, USA); RNA Integrity Number (RIN) values were greater than 8.5 for all samples. After total RNA extraction, mRNA was enriched by Oligo (dT) beads. Sequencing libraries were prepared using an Illumina TruSeq RNA Library Preparation Kit (Illumina, CA, USA) as per the manufacturer's protocol and sequenced on an Illumina HiSeq X platform, and 150 bp paired-end reads were generated.