Illumina HiSeq 2000 sequencing; The comparison of exosomal miRNAs from ECM or media derived from HFF-CBD-Lamp2b cells, HFF cells, HFF-Lamp2b cells and hUCMSCs
The comparison of exosomal miRNAs from ECM or media derived from HFF-CBD-Lamp2b cells, HFF cells, HFF-Lamp2b cells and hUCMSCs
We used repeated freeze-thaw approach to obtain exoomes that entrapped in ECM; We used ultracentrifugation-based methods to obtain exoomes that secreted in supernatant Total RNA was extracted and purified using the mirVanaTM miRNA Isolation Kit without phenol (Ambion, USA) TruSeq Small RNA Sample Prep Kits (Illumina, San Diego, USA) were used to construct miRNA sequencing libraries