GSM5584038: Anterior-pituitary only; Rattus norvegicus; RNA-Seq
Pituitary glands were quickly collected from postpubertal rats and dispersed using a trypsin/EDTA based method. 30 whole pituitaries in one preparation and 30 dissected anterior/posterior pituitaries were collected, pooled, dispersed and counted, and were loaded into the Chromium Single Cell Controller (10X Genomics). The whole pituitary cell preparation was split into two lanes, while anterior and posterior cells were each loaded into separate lanes for a total of four lanes. Single-cell cDNAs and barcoded sequencing libraries were constructed from the output of the Chromium Single Cell Controller using a Chromium Single Cell 3' Reagent Kit v3 according to the manufacturer's protocol (10X Genomics) Each library received a different sample index; these were multiplexed and sequenced together. Barcodes = cell barcodes. Barcode appendices are used to distinguish samples. The appendix ID for each sample is included in the 'description' field.