GSM5782085: 100% resistant (monoculture); Homo sapiens; RNA-Seq
After 11 days, speroids were harvested, washed and cell suspensions were viably frozen for further processing. Once thawed, cells were centrifuged at 300 x g washed twice with in 37°C pre-warmed 1x PBS, pH 7.4 (Gibco, Cat #10010) + 0.04% Nuclease-Free Bovine Serum Albumin (BSA, EMD Millipore, Cat # 12661525mL).  Cells were resuspended to a target concentration of 1,000 cell/µL, concentrations were confirmed using trypan blue staining and counted on a hemocytometer. scRNA-Seq was performed on resuspended cells using the 10X Genomics Chromium Single Cell 3' GEM, Library, & Gel Bead Kit v3 (10X Genomics, Cat # 1000075) according to manufacturer instructions at a target of 10,000 cells per sample.  Each sample was barcoded with a unique i7 Index during library preparation using the Chromium i7 Multiplex Kit (10X Genomics, Cat# 120262) to allow sample multiplexing during sequencing.