mRNA-Seq of Alysiella filiformis on mod. PY plate 24h, replicate 1
Total RNA extraction using Qiagen RNeasy mini kit including on-column DNase treatment, in-tube TURBO DNA-free DNase treatment, and purification using Zymo RNA Clean & Concentrator-25 kit. DNA digestion checked with PCR. rRNA depletion using facility-developed protocol, stranded library prep (RF/fr-firststrand stranded) using NEBNext Ultra II Directional RNA Library Prep Kit.