Selfing in Tolpis on Graciosa
We constructed RADseq libraries using the multiplexed shotgun genotyping protocol (Andolfatto et al. (2011) for sequencing using methods previously adapted for use in Tolpis (Mort et al., 2015). Briefly, we used the restriction enzyme AseI (NEB Biolabs, Ipswich, MA) and size selected for fragments between 250 and 300 bp in length. Sample-specific 6 bp barcodes were ligated to the digested fragments and barcoded DNA was then pooled and sequenced across