NextSeq 550 paired end sequencing; Cervical Adenocarcinoma Whole Exome sequencing
Cervical Adenocarcinoma Whole Exome sequencing
17 paired and 1 orphan tumor were collected from ACTREC-TMC. DNA was extracted from tumor tissue and the matched normal tissue or blood using DNeasy tissue extraction kit (Qiagen) and QIAamp DNA blood mini kit (Qiagen) following manufacturer's instruction. Genomic DNA was sheared using covaris to generate 150-500 bp fragment size. The fragment ends were repaired followed by adenylation at 3'end and sample was purified using AMPure XP beads. The fragments were ligated to adaptor and amplified by PCR. The generated library is then hybridized with SureselectTarget Enrichment system kit and hybrids are separated using streptavidin coated magnetic beads. Then the sample was PCR amplified using indexing primers and purified. The prepared libraries were loaded on Illumina flowcell to generate clusters