16S rRNA gene V1-V3 sequencing of gingival crevicular fluid from proband: 09
Amplification of 16S rRNA gene V1-V3 region using 12 phased primer pairs derived from 27Fmod: 5 AGRGTTTGATCMTGGCTCAG 3 and 519R: 5 GWATTACCGCGGCKGCTG 3 primers. 2nd Indexing PCR using Illumina Nextera v2 system