sequences of bacteria
Feces of 24-week-old mice were collected 20 weeks after immunizationTotal genomic DNA was extracted using DNA Extraction Kit following the manufacturers instructions.For bacterial diversity analysis, V3-V4 (or V4-V5) variable regions of 16S rRNA genes was amplified with universal primers 343 F and 798 R (or 515F and 907R for V4-V44 regions).