Nanoplastics shape microbiome and functional metabolism
10 mL of each digested sludge sample was obtained for DNA extraction using a PowerSoil DNA Isolation Kit (MoBio Laboratories Inc., Carlsbad, USA). Partial fragments of the 16S rRNA gene were amplified using universal primer sets: 338F and 806R for the V3V4 region of bacteria. The Illumina Nova 6000 platform was used to sequence 16S rRNA gene amplicons.