GSM4824583: Mock-infected HK-2-rep2; Homo sapiens; RNA-Seq
Total RNAs were extracted by TRIzol (ThermoFisher). Fifty nanograms of each library was then first purified by AMPure XP (Beckman Coulter) before end-repairing. Next, the 3' end of the libraries were ligated to adaptors using xGen Dual Index UMI Adapters (IDT) according to the manufacturer's protocol. The adaptor-ligated libraries were then validated by Agilent Bioanalzyer, Qubit and qPCR for quality control analysis. After, the adaptor-ligated libraries were pair-end sequenced using NovaSeq 6000 (Illumina). KAPA Hyper Prep Kit (KAPA Biosystems)