GSM5229455: CH12_PROCap_WT; Mus musculus; OTHER
Cells were harvested and nuclei isolated. Transcription run-on in the presence of all 4 biotynilated NTPs.After trizol extraction of total RNA total extraction with trizol, labeled RNA enrichement .3' and 5' linker attachment. cDNA preparation and PCR Single molecule indexing with random 10-mers via adapter ligation.TrusSeq indexed primers for multiplexing were used in the PCR step to generate barcoded libraries