GSM5665681: sigF_NT_r1; Bacillus subtilis; RNA-Seq
Upon sampling, we immediately treated bacteria with the RNAprotect Bacteria reagent (Qiagen), and stored pellets at -80 °C for <1 week before RNA extraction using RNeasy Protect Bacteria Mini Kit (Qiagen) according to the manufacturer's instructions (protocol #5), including an on-column RNase-free DNase digestion. Libraries were constructed using the Illumina TruSeq Stranded mRNA HT kit following depletion of rRNA using Illumina Ribo-Zero Plus kit. RNAseq on Illumina NextSeq 500 platform as paired end reads (2 x 38 bp)