GSM5006257: non-treated yeast, biol rep2; Saccharomyces cerevisiae; OTHER
The non-treated and telithromycin treated cells were collected by rapid filtration through membrane filter (Millipore), frozen in liquid nitrogen and processed for ribosome profiling as described in Becker et al., 2013 (for samples None1 and Teli1) or McGlincy and Ingolia, 2017 (for samples None2 and Teli2) The library for next-generation sequencing was prepared using RNA fragments ranging in size from 20 to 35 nt. Analysis of ribosome profiling was carried out using the GALAXY pipeline. The first position of the P site codon was assigned at the 3' end of the read.