GSM5203592: B. burgdorferi B31-A3△bbe02△bbq67 rep2 Sample 11; Borreliella burgdorferi; RNA-Seq
RNA was isolated using TRIzol reagent per the manufacturer's instructions and treated with 1 unit of DNAse I for 1 hr at 37 C cDNA libraries were synthesized using Ribo-Zero and ScriptSeq complete bacteria kit with indexing primers for synthesis of directional libraries per the manufacturer's instructions. The quality and quantity of the resulting cDNA libraries were assessed with an Agilent DNA 1000 assay on an Agilent 2100 Bioanalyzer and with a KAPA Library Quantification Kit prior to RNA-seq.