Pacbio library assembly of Dastarcus helophoroides: female pupae
As for long-read sequencing, a 20-kb single-molecule real-time bell (SMRTbell) library was generated using SMRTbell Template Prep Kit and sequenced with PacBio Sequel instrument according to standard protocol (Pacific Biosciences, Menlo Park, CA, USA). Briefly, high molecule genomic DNA was sheared to ~20 kb targeted size, followed by damage repair and end repair, blunt-end adaptor ligation, size selection, and binging to polymerase. Then, the long-read sequencing was performed on a PacBio Sequel platform with the P6-C4 sequencing reagent.