GSM5782182: Ghurrah Pre-challenge MDM  (DC2-3); Sus scrofa; RNA-Seq
At 48 hpi, the MDMs were harvested from the culture plates. RNA isolation from MDMs was performed using TRIzol method. The quantification of viral load in MDMs of two groups (Ghurrah and Landrace) was undertaken via absolute quantification with qPCR using primers that were specific to 5'UTR. Library preparation was done through Illumina Truseq RNA sample preparation kit V2 (PolyA capture).