GSM5006051: GA Muscle 122 EV4; Mus musculus; RNA-Seq
Homogenized GA tissue (~20 mg) was used to extract RNA by using 1 ml of Trizol reagent (Invitrogen). The RNA pellet was dissolved in RNase-free water, and subjected to library construction and deep sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols by Novogene Co., Ltd. mRNA is purified from total RNA using poly-T oligo-attached magnetic beads. The mRNA is first fragmented randomly by addition of fragmentation buffer.