GSM5897112: RWPE1 6hr Hypoxia rep3; Homo sapiens; RNA-Seq
RNA was isolated using the Qiagen RNeasy mini kit (catalog number 74104). Genomic DNA was removed from samples using Qaigen RNase-Free DNase Set (catalog number 79254) as instructed in the RNeasy protocol RNASeq builds were performed on an Apollo 324 Automation Platform in conjunction with an illumina Truseq Stranded RNA build kit and combinatorial dual indexing. The 12 samples were built in a single batch. The 12 samples were pooled equally and run on our NextSeq 500 instrument using a High Output 75 cycle sequencing run with single-ended reads