GSM5856817: CFL_3; Astyanax mexicanus; RNA-Seq
According to manufacturer's imstructions Qiagen RNeasy RNA mini kit. RNA was extracted using Qiagen RNeasy kit according to manufacturer's instructions. Libraries were generated from 250ng of high-quality total RNA, as assessed using the Bioanalyzer (Agilent).  Libraries were made according to the manufacturer's directions using the TruSeq Stranded mRNA Library Prep kit (48 Samples) (Illumina, Cat. No. 20020594), and TruSeq RNA Single Indexes Sets A and B (Illumina Cat. No. 20020492 and 20020493). Resulting short fragment libraries were checked for quality and quantity using the Bioanalyzer (Agilent) and Qubit Fluorometer (Life Technologies). Libraries were pooled, quantified, and sequenced as 75bp single reads on a high-output flow cell using the Illumina NextSeq 500 instrument. Following sequencing, Illumina Primary Analysis version RTA 2.4.11 and bcl2fastq2 v2.20 were run to demultiplex reads for all libraries and generate FASTQ files.