Double-digest genotype-by-sequencing of field and herbarium leaf tissue of Paubrasilia echinata
Library preparation and sequencing was carried out by the institute of integrative biology and systems (IBIS) at the University of Laval, Canada, using a modified GBS approach with single end reads and two restriction enzymes: a rare cutting enzyme PstI (CTGCAG) and a common cutting enzyme MspI (CCGG) (Poland et al., 2012).