GSM5418105: hTSC Input rep1; Homo sapiens; ChIP-Seq
Human ESCs and human TSCs were dissociated with Accutase (Thermo Fisher Scientific, catalog. no. A1110501) into single cells and washed with PBS solution twice. Then, hESCs and hTSCs were collected into the nuclear isolation buffer. Lysates were clarified from micrococcal nuclease-treated nuclei and histone-DNA complexes were isolated with anti-H3K9me3 antibody. Libraries were prepared according to the Accel-NGS  2S Plus DNA Liibrary Kit from Swift Biosciences.