Whole metagenome cave surface
Paired-end sequencing libraries were prepared from the extracted DNA using the Illumina Nextera XTLibrary Preparation Kit (Illumina Inc., San Diego, CA) followed by sequencing on the Illumina NextSeq 500, with a NextSeq 500/550 High Output Reagent kit v2 (300 cycles), in accordance with the standard Illumina sequencing protocols.