ChIP-PCR on the pathway reporters in K562 TRIP clone 5 on sample: indelPCR_RSTP2_clone_5_EV_72h_R1
CRISPR/Cas9 mutation readout on the pathway reporters in K562 TRIP clone 5 for the DDR ChIP experiment after were transfected with sgRNA LBR2 or an empty vector, 8 hours later Cas9 was activated, 16 hours after activation the cells were collected and processed for ChIP but for these samples the left over was kept in the incubator for a total of 72 hours after activation. The samples were lysed and amplified by two successive PCRs on the integrated pathway reporters to generate the libraries, spanning the sgRNA target site and the barcode