GSM5418488: High back fat rep1; Sus scrofa; RNA-Seq
Total RNA was extracted from the LD muscle and back subcutaneous fat tissues according to the TRIzol method. RNase-free DNase was added to total RNA to remove DNA contamination. The integrity of RNA was checked by 1.2% agarose gel electrophoresis and the quality of RNA and the amount of total RNA were determined by Nanodrop 2000 RNA libraries were prepared for sequencing using standard Illumina protocols