GSM5646546: miR-29a KO-1; Mus musculus; RNA-Seq
After the mice were euthanized, total RNA Kit II (Omega Bio-tek, Norcross, GA, USA) was used to extract the total RNA from retinas isolated from wild-type (WT) and miR-29a KO mice. Retinas from three mice were pooled as one sample. T10 basic S25 ULTRA-TURRAX Disperser (IKA, Guangzhou, China) was used to homogenize retinas. The quantity and integrity of RNA yield was assessed by using the NanoPhotometer spectrophotometer (Implen, Westlake Village, CA, USA) and Agilent 2100 bioanalyzer (Agilent Technologies, USA) separately. cDNA libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, Ipswich, MA) after removed the ribosomal RNA by Ribo-Zero kit (Epicentre, Madison, WI) according to the manufacturer's recommendations. The purified library products were evaluated using the Agilent 2200 TapeStation and The Qubit 2.0 Fluorometer (Thermo Fisher Scientific). The libraries were sequenced at Novogene Co., Ltd. (Beijing, China) using Illumina HiSeq 3000 platform.