WGS of homo sapiens: adult PBMC
Genomic DNA extracted from peripheral blood was fragmented to an average size of ~350bp and subjected to DNA library creation using established Illumina paired-end protocols. The Illumina Novaseq 6000 platform (Illumina Inc., San Diego, CA, USA) was utilized for genomic DNA sequencing in Novogene Bioinformatics Technology Co., Ltd (Beijing, China) to generate 150-bp paired-end reads with a minimum coverage of 10 for ~99% of the genome (mean coverage of 30).