GSM5418086: NEK2-KD rep2 [K002000164_74479]; Homo sapiens; RNA-Seq
U3047MG (before ciliation) and U3047-Nek2-KD (after ciliation) cells were collected and washed once with cold PBS before adding to 300 µl Tri-Reagent (Sigma Aldrich, USA) by free-thawing, and total RNA was isolated and DNase-treated using the DirectZol RNA kit (Zymo Research). Approximately 2 μg of total RNA was used to subselect poly(A)+ transcripts and generate strandspecific cDNA libraries (TrueSeq kit; Illumina). TruSeq RNA library kit (Illumina)