GSM4880146: IgG 2; Mus musculus; RNA-Seq
Spleens were harvested; B cells were enriched using B220 magentic beads (Bd Biosciences) and then sorted for CD19+GFP+ (IL10+). Libraries were prepared with the MARS-seq protocol (Diego Adhemar Jaitin, 2017) and sequenced using Illumina NextSeq 550 High Output v2 Kit (75 cycles).