GSM5050099: bone marrow cells, untreated; Mus musculus; RNA-Seq
Bone marrow cells from one tibia and one femur per mouse were harvested 3 days after initiation of therapy. Erythroblast cells were magnetically removed using anti-Ter-119 biotin-labeled monoclonal antibodies and streptavidin-labeled magnetic beads (Mojosort streptavidin nanobeads, BioLegend). scRNA librairies were prepared for sequencing using standard 10x protocol (Single Cell 3' Reagent Kit v3) single cell RNA-Seq