GSM4780394: DHTS_rep1; Mus musculus; RNA-Seq
After 24-h treatment with either DMSO or DHTS, total RNA was extracted from EOMA cells using TRIzol Reagent (15596026; Invitrogen, Carlsbad, CA, USA). A total of 3 µg of RNA per sample was used as input material for RNA sample preparations. Sequencing libraries were generated using a NEBNext® UltraTM RNA Library Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer's recommendations and library quality was assessed on an Agilent Bioanalyzer 2100 system (Santa Clara, CA, USA). Sequencing was performed on Illumina HiSeq platform at Beijing Novogene (Beijing, China).