GSM5290665: Mouse lung Mock-1; Mus musculus; RNA-Seq
The lung tissue was dissociated into single-cell suspensions by enzymatic degradation using Lung Dissociation Kit (mouse) (Miltenyi Biotech) according to the manufacturer's protocol, followed by mechanical dissociation using gentleMACS™ Dissociators (Miltenyi Biotech​).  Low quality single cells were then depleted by MACS® Separation using Dead Cells Removal kit (Miltenyi Biotech).  Single cells suspension were then loaded into a Chromium Single Cell Chip G (10x Genomics) according to the manufacturer's instructions for co-encapsulation with barcoded Gel Beads at a target capture rate of ~5,000 individual cells per sample.  