GSM4729851: BMDC SIP-H3K4me3 3; Mus musculus; OTHER
Cells were crosslinked with 3%FA-DSG and then lysed with nuclei isolated. DNA was then lightly sonicated and DNAse digested to an average size of approximately 150-1000 bp in length. Crosslinked protein-DNA complexes were isolated on NHS beads, DNA ends were repaired, dA-tailed to generate a protruding 3' A-base for ligation of barcodes, and were then split-pool tagged with a series of barcodes over multiple rounds of split-and-pool tagging. After split-and-pool tagging, crosslinks were reversed, the DNA was isolated, was amplified 8-12 cycles using standard Illumina library prep (single index), and purified using SPRI beads. Libraries were sequenced on the NextSeq 500 following the manufacturer's protocols.