Hydrophis curtus gut microbiota 16S
Total DNA was extracted from the gut contents of each snake using the CTAB method. The purity and concentration of the DNA were detected by agarose gel electrophoresis. The DNA diluted to 1 ng/l with sterile water was used as the template for the amplification of the V4 and V3 regions of the 16S rRNA genes using the primers 341F (5-CCTACGGGNGGCWGCAG-3) and 806R (5-GGACTACHVGGGTATCTAAT-3). The PCR products were then sequenced on Illumina PE250 using Single-End.