GSM5050316: hgsc_01; Homo sapiens; RNA-Seq
RNA was extracted from individual fresh snap-frozen tissue samples using TRIzol (Invitrogen) according to the manufacturer's protocols. mRNA was purified and fragmented from total RNA (1ug) using poly-T oligo attached magnetic beads. RNA sequencing libraries were prepared using Illumina Truseq stranded mRNA Prep kit according to the manufacturer's instructions. Cleaved RNA fragments primed with random hexamers were reverse transcribed into first strand cDNA. These cDNA fragments then had a single 'A' base added and were subsequently ligated to the adapter. The products were purified and enriched with polymerase chain reaction to create the strand specific cDNA library.