GSM4824426: CAS_TF.SingleFactor.Unsort.Rep3; Homo sapiens; OTHER
After sorting, genomic DNA was harvested with the DNeasy Blood and Tissue Kit (Qiagen 69506) The gRNA libraries were amplified from each genomic DNA sample across 100 μL PCR reactions using Q5 hot start polymerase (NEB, M0493) with 1 μg of genomic DNA per reaction. The PCR amplification was done according to the manufacturer's instructions, using 25 cycles at an annealing temperature of 60 °C