GSM5203394: OA3; Homo sapiens; RNA-Seq
Articular cartilage obtained from non-arthritic and OA patients was finely minced under sterile conditions and digested with 0.25% Collagenase type II from Clostrudium histolyticum (Sigma-Aldrich) dissolved in DMEM at 37oC overnight. After enzymatic digestion, cells were pelleted by centrifugation, washed, filtered through 50uM cell strainer, and resuspended in 1ml PBS. The scRNA-Seq libraries were generated using the 10X Genomics Chromium Controller Instrument and Chromium Single Cell 3' V3 Reagent Kits (10X Genomics, Pleasanton, CA) and sequenced the Illumina HiSeq 4,000 with read length of 150 bp by the NYU Genome Technology Center.