16S amplicon sequencing of Isochrysis galbana microbiome run 1
Microbiome of Isochrysis galbana captured in attached and free-living fraction on 5 and 0.2 micron PC filters. DNA extracted with phenol-chloroform, Nucleospin Tissue Kit, DNeasy PowerWater Kit, and DNeasy PowerSoil Kit. Then, PCR of V3-V4 region of 16S rRNA gene using primers 341f (5'-CCTACGGGNGGCWGCAG-3') and 805r (5'-GACTACHVGGGTATCTAATCC-3') with barcodes.