Illumina HiSeq 4000 sequencing; RNA-seq of stomach and pyloric caeca from Atlantic salmon (Salmo salar) fed diets containing chitin from shrimp and black soldier fly larvae
RNA-seq of stomach and pyloric caeca from Atlantic salmon (Salmo salar) fed diets containing chitin from shrimp and black soldier fly larvae
All fish was killed with a sharp blow to the head after four weeks. The following tissues/content were sampled: feces (Inga Angell), liver, stomach, pyloric caeca, midgut (1 cm below last caeca) and spleen. The samples were flash frozen in liquid nitrogen and stored at -80 for long term storage. The fish had not been starved prior to sampling. Total RNA was extracted with Qiagen RNeasy Plus Universal Kit. RNA quality was measured with Nanodrop8000 and Agilent Bioanalyzer 2100.  Library preperation was done with TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA).