GSM5050654: Tconv_dLN_Ctrl mouse rep3; Mus musculus; RNA-Seq
RNA was extracted from highly purified Treg and Tconv cells using the NucleoSpin RNA XS kit from Macherey-Nagel, quantified using a ND-1000 NanoDrop spectrophotometer (NanoDrop Technologies) and purity/integrity was assessed using disposable RNA chips (Agilent High Sensitivity RNA ScreenTape) and an Agilent 2200 Tapestation (Agilent Technologies, Waldbrunn, Germany). Libraries were prepared from the extracted RNA using the Smartseq V4 TAKARA followed by Nextera library preparation