GSM5049915: R2_jy90: pnp-1(jy90) replicate 2; Caenorhabditis elegans; RNA-Seq
Total RNA was extracted using TriReagent (Sigma-Aldrich) and cleaned using RNAeasy (Qiagen) column purification. RNA quality was assesed using Tapestation analysis. Paired-end sequencing libraries were constructed using TruSeq stranded mRNA TruSeq Stranded mRNA (Illumina) prep kit.