Nicotiana tabacum cultivar: 94 tobacco accessions raw sequence reads
For genotype-by-sequencing (GBS) library preparation, genomic DNA was digested with the restriction enzyme ApeK1, and libraries with 250500 bp were constructed in 96-plex using the protocols developed by Elshire et al. (2011). The GBS libraries were sequenced on an Illumina HiSeqTM 2000 instrument. Raw reads were de-multiplexed using a barcode sequence, and the adapter sequences were trimmed using the standard Illumina GA Pipeline v1.5.