mRNA-Seq of M.tuberculosis : H37Rv
Sample quality was assessed by Agilent RNA 6000 Pico Reagent on Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA) and quantified by Qubit RNA HS assay (ThermoFisher, Waltham, MA). Library preparation was performed with NEBNext Ultra II with RiboZero Plus Prep Kit (Illumina Inc., San Diego, CA) following manufacturers instructions. Average final library size is 150bp. Samples were pooled and sequenced on HiSeq sequencer for 150 bp read length in paired-end mode, with an output of 30 million reads per sample