GSM5049713: RNA-Seq of HEK293 cells after knockdown of SP1, replicate 1; Homo sapiens; RNA-Seq
RNA was isolated from lysates using Trizol (Thermo Fisher Scientific) as described by the manufacturer and was treated with DNAse (Thermo Fisher). 1000 ng per sample was used in the library preparation. Sequencing libraries were constructed using Illumina's TruSeq Stranded mRNA sample preparation protocol, as described in Illumina's TruSeq mRNA sample preparation guide.