Cv2vegControl
The library preparation was designed to remove the ribosomal RNA prior to sequencing. This increases the depth of sequencing of the transcriptome since ribosomal RNA accounts for vast majority of the transcriptome. Ribo-Zero protocol  (ILLUMINA PROPRIETARY Part # 15065382 Rev A November 2014) was used for the qualification, and sequencing of RNA according to the procedure of Chenchik et al., (1993); Ramskold et al., (2012) and Picelli et al., (2014).SequencingThe library pool(s) to be sequenced are denatured and diluted/neutralized in the required concentrations Then cluster generation is performed on the appropriate flow cell using single molecule clonal amplification. Finally, the high- throughput next generation sequencing is performed using the Illumina sequencing technology platform. For more detailed description of the sequencing process please visit the Illumina homepage at www.illumina.com