RNA-seq of Phrynus marginemaculatus: pedipalp
TruSeq Stranded mRNA Sample Preparation Guide Rev. E (HS); Stranded rgts removed from -20C and thawed according to protocol directions; Protocol: (20); Target 100ng total RNA input using Qubit concentrations; Poly-A selection; Fragment, Prime, Finish using FPF mix (Frag 5 minutes); First strand & second strand cDNA Synthesis; Agencourt AMPure XP beads bead purification (lot#18554100); Plate stored at -20C; 12/9/2020 AF; Plate removed from -20C, thawed on ice and quick spun; Adenylate 3 Ends; Ligation-Illumina 1:2 UDI dilutions, lot #20418535; 0.8X Agencourt AMPure XP beads bead purification (lot#18554100) 2x; PCR (15 cycles); 0.8X Agencourt AMPure XP bead purification (lot#18554100); Added 32.5ul RSB & recovered 30uL of purified library; Library QuantificationEach quantified in singlet with Qubit 1X HS dsDNA working solution, lot # 2240357 with 1:100diluiton; 12/10/2020 AF; Libraries assayed on Agilent Tapestation 4200 with D1000 screen tape reagents, lot #6552187, D1000 screen tapes, lot; #201886; 12/15/2020; Libraries (20) submitted to the DNA Sequencing Core in strip-tubes