PromethION sequencing
Oxford Nanopore Technology sequencing for genome assembly
DNA from the hybrid S. lycopersicum cv. Funtelle was extracted using the Machery Nagel Nucleobond HMW DNA Kit. High molecular weight DNA was size selected using the Circulomics Short-Read Eliminator XL Kit (Circulomics Cat# SKU SS-100-111-01) and used as input for a library preparation with the Oxford Nanopore LSK-110 ligation sequencing Kit. Four libraries from the same sample were loaded onto three PromethION PRO-002 Flowcells and one MinION Flowcell (all R9.4.1 pores) and sequenced. ONT Sequencing was performed on an Oxford Nanopore P24 PromethION and a MinION MK1B at the Forschungszentrum JÃ¼lich using MinKNOW version 21.02.7 without live basecalling. Basecalling of the Oxford Nanopore read data was done using guppy basecaller version 5.0.11 using the R9.4.1 PromethION super-high-accuracy model (dna_r9.4.1_450bps_sup_prom) and the R9.4.1 MinION super-high-accuracy model (dna_r9.4.1_450bps_sup).