NextSeq 500 paired end sequencing; Dynamic in vivo labelling reveals the continuous trafficking of stem-like TCF1+ CD8 T cells through tumours
Dynamic in vivo labelling reveals the continuous trafficking of stem-like TCF1+ CD8 T cells through tumours
A total of 13 Kaede B6 females (aged between 7-9 week old at tumour injection, and 9-11 week old when tumours were photo converted) were subcutaeneously injected 2.5x 10^5 MC38 cells in the flank and photo converted on D11. 24 and 72 hrs after photo conversion 4 tumours were collected for each time point. FACS sorted lymphocytes were isolated from tumors of transgenic mice. Used 10X sample collection protocol, detailed in CG000183_ChromiumSingleCell3__v3_UG_Rev-A Used 10X sample collection protocol, detailed in CG000183_ChromiumSingleCell3__v3_UG_Rev-A