GSM6333154: BLA, ER, biol rep 2; Rattus norvegicus; RNA-Seq
RNA was harvested using RNAqueous-Micro isolation kits (Qiagen). ~400ng of total RNA was used for the construction of sequencing libraries.  Samples underwent polyA RNA isolation using the NEBNext Poly(A) mRNA Magnetic Isolation Module prior to library preparation. RNA libraries for RNA-seq were prepared using the NEBNext Ultra Directional RNA Library prep kit following manufacturer's protocols.