GSM5418833: Control 1; Aspergillus flavus; RNA-Seq
Fungal mycelia were removed from 4 days old cultures and grinded to fine powder in liquid nitrogen. The cell mass was supplemented with 1 µl RiboLock RNase inhibitor and immediately used to extract RNA with Trizol Reagent according to the manufacturer´s instructions. cDNA libraries were prepared and sequenced at Novogene company (Cambridge, UK) using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (New England Biolabs, USA) and index codes were added to attribute sequences to each sample.