GSM5822730: 15-day old cecum control 1; Mus musculus; miRNA-Seq
The cecum samples aseptically collected from chickens were thoroughly rinsed in PBS and immediately frozen in liquid nitrogen. Then the cecum samples were stored at −80°C until RNA extraction.Total RNA was prepared from individual cecum samples using TRIzol Reagent according to the manufacturer's instruction. The small RNA libraries were prepared from a total of 2 μg total RNA isolated from each sample using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® smallRNA libraries were prepared for sequencing using standard Illumina protocols