Illumina HiSeq 1000 sequencing; Response of RAW 264.7 cell line to treatment with extracellular vesicles isolated from brown adipocytes
Response of RAW 264.7 cell line to treatment with extracellular vesicles isolated from brown adipocytes
Cells were detached by scraping in cell culture medium, collected through centrifugation and stored at -80 prior to RNA etraction Total RNA was isolated with TRIzol Reagent (Invitrogen) and purified using the RNeasy mini kit protocol (Qiagen), according to the manufacturer's instructions.  The libraries were prepared with a TruSeq Stranded mRNA (Illumina) Library Prep kit according to the manufacturer's instructions