Environmental DNA sequences collected from soil
DNA was extracted from 2g of dried soil using the PowerMax(R) Soil DNA Isolation Kit. Prokaryotic primers 515F (5-GTGYCAGCMGCCGCGGTAA-3) and 926R (5-GGCCGYCAATTYMTTTRAGTTT-3) were used to amplify the 16S rRNA variable V4 region. Both primers were equipped with unique 12-base Golay barcodes for multiplexing.