GSM5049923: R5_jy121C: pnp-1(jy121) replicate 5; Caenorhabditis elegans; RNA-Seq
Total RNA was extracted using TriReagent (Sigma-Aldrich) and cleaned using RNAeasy (Qiagen) column purification. RNA quality was assesed using Tapestation analysis. Paired-end sequencing libraries were constructed using TruSeq stranded mRNA TruSeq Stranded mRNA (Illumina) prep kit.