LPS2
Chondrocytes were isolated from tibial plateau cartilage of rats. Chondrocytes were cultured in DMEM supplemented with 10% FBS and antibiotics (100 U/ml penicillin, 100 ug/ml streptomycin) at 37C in a humidified 95% air and 5% CO2 atmosphere for 7 days. Then, chondrocytes were stimulated by  lipopolysaccharide for 6 hours.