Sequence capture of Acrocephalus luscinius: USNM383057
Libraries were prepared from DNA extracted from historical museum samples in a dedicated ancient DNA laboratory using double indexed Illumina libraries using KAPA Library Preparation kits (Kapa Biosystems, Wilmington, MA, USA) and iNext adapters (Faircloth & Glenn, 2012). Targeted sequence capture was performed on pooled libraries using a custom-designed Acrocephalus specific sequence capture kit (some samples underwent a separate sequence capture targeting the mitogenome using a pre-designed myBaits mito capture kit). Capture pools were pooled and sequenced using 150 bp paired-end on NovaSeq SP (University of California, Berkeley).