GSM5550791: Mdm2Ex5/6Δ HOM; AAV-Cre 3 [CKR9180804_S31_CK31_RMG33_1c_L]; Mus musculus; RNA-Seq
Liver samples were isolated and snap frozen on dry ice and stored at -80°C until RNA extraction. To isolate RNA, tissue was homogenised using a Precellys tissue homogeniser and RNA was extracted using the RNeasy Plus Universal mini kit (Qiagen). Libraries were prepared using an Illumina TruSeq Stranded mRNA HT Kit according to manufacturer's instructions.