GSM4823365: HC05; Homo sapiens; RNA-Seq
Whole blood was collected, erythrocytes were lysed with Buffer EL (Qiagen) and total RNA was isolated from the leukocytes with the miRNeasy Mini Kit (Qiagen) following the manufacturer's instructions. After RNA quality control and quantification, rRNA was depleted from the total RNA sample using the Ribo-Zero rRNA removal kit and followed by purification and fragmentation steps. To construct the sequencing libraries, a strand-specific cDNA synthesis was performed, the 3' ends were adenylated and adaptors were ligated. The resulting libraries were subjected to quality control and normalization process. Paired-end sequencing was performed with Illumina HiSeq PE150 and an average of 10Gb of data were obtained per sample