WGS of M42015
Genomic DNA was prepared for sequencing using the 5 Prime ArchivePure DNA Purification kit (Gaithersburg, MD) or Gentra Puregene yeast/bacteria DNA extraction kit (Qiagen, Germantown, MD) as previously described(34) for sequencing on Pacific Biosystems (PacBio; Menlo Park, CA, USA) and/or Illumina platforms (HiSeq2500 or MiSeq; San Diego, CA, USA); genomic DNA was further prepared for Illumina sequencing with the dual-index NEBNext Ultra DNA library preparation kits (New England Biolabs Inc.; Ipswich, MA).