GSM6276352: Ad-OSCAR + 5-ASA treatment rep 3; Mus musculus; RNA-Seq
For primary cell culture, chondrocytes were isolated from the femoral condyles and tibial plateaus of postnatal day 5 WT mice. RNA was harvested using PureLink RNA Mini Kit (Invitrogen). 1.9 ug of total RNA was used for the construction of sequencing libraries. Sequencing libraries were prepared according to the manufacturer's instructions (Illumina Truseq stranded mRNA library prep kit).