GSM5551478: ATAC-Seq MGT_siAJSZ_1; Homo sapiens; ATAC-seq
Cells were collected 2 days later using trypsin-EDTA. Cells from 40 wells were pooled to obtain ≥2×105 cells per sample, with two biological replicates per condition. Cells were washed with FCM, centrifuged in conical tubes, and the pellets were frozen in Freezing Medium (DMEM, 10% DMSO, 20% FBS), transferred to cryotubes, and placed in Mr, Frosty containers (Thermo Fisher) at −80°C. Samples were processed for scATAC-seq at UCSD CMME. Samples were processed for scATAC-seq at UCSD CMME using 10x Genomics Libraries were sequenced on a NovaSeq 6000 at a depth of 20-25k read pairs per nucleus