GSM5597364: CB-HSPC - CH223191_rep1; Homo sapiens; RNA-Seq
Total RNA is manually extracted using Trizol from lysates of cultured HSPCs. Using Illumina TruSeq Stranded mRNA Sample Preparation kit, mRNA sequencing library were prepared. Library size and quality were confirmed by Agilent 2100 bioanalyzer DNA kit (Agilent). Libraries were quantified (qPCR using CFX96 Real Time System, Biorad) and equenced on the NextSeq500 sequencers (Illumina).