Hi-C library assembly of Dastarcus helophoroides: female pupae
A Hi-C fragment library was constructed for D. helophoroides DNA according to the improved Hi-C procedures. After treatment with a 1% formaldehyde solution in phosphate-buffered saline and digestion with DNase I, the DNA ends were labeled with biotin. Then, the DNA ligation was added of T4 DNA ligase (NEB) and proteinase K, and un-ligated ends of DNA fragments were removed. The purified DNA was fragmented to a size of 300-500 bp, and followed with DNA ends repair. The repaired DNA fragments labeled by biotin were finally separated on Dynabeads M-280 Streptavidin (Life Technologies). Hi-C fragment libraries sequencing was performed using the Illumina NovaSeq 6000 Sequencing System (Illumina Inc., San Diego, CA, USA) with a 150-bp paired-end strategy.