RNAseq of the whole venom duct of Virroconus ebraeus
RNAseq of the venom duct from Virroconus ebraeus from Sesoko Island Beach, Okinawa, Japan. The specimen was dissected and the whole venom duct extracted, which was preserved in RNALater (Invitrogen, Life technologies). Once in the lab, it was incubated in TRIzol LS Reagent (Invitrogen, Life Technologies) and grinded with ceramic beads in a Praecellys Evolution homogenizer. Total, RNA was purified using the Direct-Zol RNA miniprep kit (Zymo Research, Irvine) following manufacturers instructions. Dual-indexed cDNA libraries were constructed for each sample using the TruSeq RNA library Prep kit v2 (Illumina, San Diego) at Sistemas Genomicos (Valencia, Spain) following manufacturers instructions.