10genomic assembly of Dastarcus helophoroides: female pupae
In 10X Genomics library construction, a small amount (1.25-1.5ng) of gDNA was partitioned across over 1 million Gel Bead-In-EMulsions (GEMs) and consequently only a small number of genomic equivalents are loaded in each GEM (~500 kb). Each Gel Bead was functionalized with millions of 10x Barcoded primer copies and approximately 50% of loaded gDNA was incorporated into the final sequencing library. Subsequently, the Chromium Genome Protocol produces Illumina sequencing-ready library containing molecules, Read 1and 2 sequences, 10x Barcode, P5 and P7 primers, Read 2, end repair, A-tailing, adaptor ligation were used in Illumina bridge amplification and for paired-end sequencing.