GSM5517034: WT_BM_HSC_3 [bisulfite-seq]; Mus musculus; Bisulfite-Seq
Genomic DNA was purified from sorted HSCs followed by bisulfite conversion and bisulfite PCR to amplify the region of interest (Tfrc gene). The PCR products were purified and prepped for amplicon-seq using the Illumina Nextera XT kit.