CRISPR/Cas9 screen in BLaER1 cells
BLaER1-Cas9 cells expressing the sgRNA library were stained with an anti-viperin antibody and FACSorted into Viperin+ sgRNA+ cells (IFN_vip) or sgRNA+ cells (IFN_ctrl). sgRNA cassettes were amplified from cell lysates utilizing a nested PCR approach to attach Illumina adapters.