wt biological replicate3
In order to explore the underlying mechanism of the increased CTCF binding, we set out to examine nucleosome occupancy by H2A CUT&Tag-seq. Since FACT is well known to promote survival of intact nucleosomes following transcriptional elongation, we supposed that Ssrp1 depletion reduces nucleosome occupancy and increases chromatin accessibility. In agreement with our prediction, overall H2A CUT&Tag signal was dramatically decreased in Ssrp1 KO cells, and the signal in all genomic elements, especially in promoter regions were markedly reduced.