GSM5006263: hiPSC-derived_microglia_RNA-seq_rep4; Homo sapiens; RNA-Seq
Cells were harvested, stored in RNAlater, and RNA subsequently extracted from all cell types using RNeasy Mini (Qiagen) following manufacturer's guidelines. RNA sequencing was performed by UCI GHTF.  The Illumina TruSeq mRNA stranded protocol was used to isolate poly-A mRNA from RNA (RNA integrity score ≥ 9) 200 ng was used to construct libraries that were quantified and normalized using the Library Quantification Kit (Kapa Biosystems) and sequenced as paired-end 100 bp reads on the Illumina HiSeq 4000