GSM5462677: 3D7_AP2-06A-KD(-)GlcN_S_RNA-seq, Rep3; Plasmodium falciparum; RNA-Seq
Highly synchronous parasites were collected in TRIzol at the ring (10–15 hpi), trophozoite (25–30 hpi), and schizont (40–45 hpi) stages from the next cycle, respectively. Total RNA purification was conducted with the Direct-zol RNA Kit (Zymo Research). Libraries were prepared for strand-specific RNA sequencing by poly(A) selection with the KAPA mRNA Capture Beads (KAPA Biosystems) first and then RNA fragmentation to a size of 300–400 nucleotides. Subsequent library preparation steps followed the protocol of the KAPA Stranded mRNA-Seq Kit Illumina platform (KAPA Biosystems, KK8421).