Metagenomic of MT sediment: sediment depth 0-5 cm
DNA concentrations of the extract were measured with a Qubit fluorometer. For each library construction, about 100 ng DNA was fragmented with Covaris S2 (Covaris, USA) and was used to construct metagenomic DNA library with NEXTflex DNA Sequencing Kit compatible with the Biomek FXp (Bio Scientific, USA). Notably PCR-amplification was limited to 12 cycles for each Illumina library. The quality of DNA library was examined by Agilent Bioanalyzer 2100 (Agilent, USA) with DNA 12000 Kit. Paired-end Illumina sequencing (2 x 150 bp) was performed for each metagenomic library on Hiseq Xten instruments (Illumina).