DCM2
Total RNAs were extracted using the RNeasy mini kit (Qiagen, Hilden, Germany). The quantity and the quantity of total RNA was measured by Nanodrop 2000 spectrophotometer (Thermo Scientific, USA), and the RNA integrity was detected using Agilent Bioanalyzer 2100 (Agilent Technologies, USA). The total amount of 3 g of total RNA with a RIN score greater than 8.0 for each sample was acceptable quality for library preparation. mRNA sequencing library prepared with a Truseq RNA Sample Prep Kit (Illumina) following the manufacturers instructions. Libraries were sequenced on an Illumina HiSeq X Ten sequencing platform by Novogene Bioinformatics Technology Co., Ltd. (Beijing, China) with a 150 bp paired-end module.