Illumina NovaSeq 6000 sequencing; ATACseq : role of trisomy 21, GATAs mutation and associated mutations in the developpment of trisomy 21
ATACseq : role of trisomy 21, GATAs mutation and associated mutations in the developpment of trisomy 21
The CD41+/CD42+ MK were sorted (10 000 to 30 000 cells).  Cells were submitted to cell lysis, transposition, and purification steps according to Illumina protocol. The transposed DNA fragments were amplified by PCR 12 times using adapters from the Nextera Index Kit (Illumina). PCR purification was performed using MinElute PCR Purification Kit (Qiagen, 28004) to remove large fragments and remaining primers. Library quality was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies 5067-4626).