GSM5005778: NS_64; Homo sapiens; RNA-Seq
Total RNA was isolated with RNeasy Kit (Qiagen) from 5mm skin biopsy that was previously powderized in liquid nitrogen. Complemetary DNA (cDNA) was prepared using the NuGEN Ovation RNA-Seq System to obtain the strand-specific RNA-seq libraries from 100 ng of total RNA.Ribosomal RNA transcripts were depleted using the Insert Dependent Adaptor Cleavage (InDA-C) technology.. Equimolar pool of the final indexed RNA-Seq libraries were sequenced on an Illumina HiSeq 2500 (paired-end reads 130 bases + 130 bases) to obtain about 50 million paired-end reads per library.