GSM4729821: 293T WT RNA-seq #2; Homo sapiens; RNA-Seq
Total RNA from the cells was prepared using the Direct-zol™ RNA MiniPrep Plus (Zymo Research Corporation) according to manufacturer's protocol. Turbo DNase (Thermo Fisher) treatment was performed according to guidelines of the producer. Quality and quantity of the resulting RNA were confirmed by RNA Nano LabChip Bioanalyzer run (Agilent). Libraries were constructed from 400 ng of total RNA using the TruSeq Stranded Total RNA LT Sample Prep (with double Ribo-Zero Gold treatment and single indexing). Quality of libraries was ensured with Bioanalyzer High Sensitivity DNA kit (Agilent). Sequencing was performed using the NextSeq 500 High Output v2 kit (150 cycles) Illumina platform.