mRNA-Seq of Mus Musculus: Adult male hypothalamus
Total RNA with RIN  8.0, was converted into astrand-specific library using Illuminas TruSeq Stranded mRNA HT Sample Prep Kit (Illumina, RS-122-2103),for subsequent cluster generation and sequencing on Illumina's NextSeq. The library was enriched by 14 cycles of PCR, validated using Agilent TapeStation and quantitated by qPCR. Individually indexed cDNA libraries were pooled and sequenced on NextSeq 75 SR