GSM5050311: C_4_S29_L001_R1_001; Solanum lycopersicum; RNA-Seq
Total RNA extraction was performed on leaf tissue harvested three weeks after psyllid infestation using the Plant RNeasy Mini Kit (Qiagen, Valencia, CA) following the manufacturer's protocol. Three biological replicates were sequenced per treatment (i.e., uninfested and psyllid-infested, six samples total). One fully-develop leaf and petiole were removed per biological replicate using sterilized razor blades. The top-most leaf was sampled to ensure that the gene expression changes observed were more likely to be associated with a plant systemic response. Samples were ground using sterilized plastic pestles. RNA samples were treated with RNase-Free DNase (Qiagen). Any remaining DNA was removed using the TURBO DNA-free™ Kit (Life Technologies, Carlsbad, CA). All remaining RNA was stored at -80°C for downstream quantitative reverse transcription PCR (RT-qPCR) validation. The isolated RNA was submitted to the Texas A&M Genomics and Bioinformatic Service for quality analysis, library preparation, and sequencing. cDNA libraries were developed using the TruSeq RNA Library Prep Kit v2 (Illumina ®; San Diego, CA) following the manufacturer's protocol, generating 2 Х 150 bp read lengths