GSM5823652: Cd45pos_Il17_rep1; Mus musculus; RNA-Seq
CD45+ and CD45–/EpCAM– cells were FACS sorted in a BD Fusion cell sorter separately to enrich for the less abundant CD45+ cells, following the sorting strategy depicted in Suppl. Fig. 1a. Cells were collected in PBS + 0.5% BSA at 4°C in LoBind tubes (Eppendorf) and processed immediately with the microfluidics Chromium platform (10X Genomics) Single-cell RNA sequencing libraries were prepared using the Chromium 3' protocol