Nanopore whole-genome sequencing of Bacillus altitudinis strain UTK D1-0055
DNA was extracted with the Qiagen DNA Easy mini kit (Qiagen GmbH) per manufacturer instructions. Libraries were prepared using the Native Barcoding Expansion and Ligation sequencing kits (Oxford Nanopore; EXP-NBD104 and SQK-LSK109) according to manufacturer protocols (version NBE_9065_v109_revE_23May2018), but substituting Magbio Genomics Inc HighPrep PCR SPRI beads for AMPure XP SPRI beads.