CGP of heterozygous S. cerevisiae pool treated with DMSO (control) for 10 generations
A pool collection of ~6000 heterozygous yeast strains, where one copy of each of the predicted S. cerevisiae open reading frames (ORF) was replaced by an antibiotic resistance marker (KANMX4) was distributed in 500 L YNB complete medium in triplicates, either with violacein EC20 or vehicle control in 48-well plates kept at 30C with agitation at 200 rpm. A fraction (1/20) of the culture was diluted in a new plate every 12 h for 24 h (~10 yeast generations). Cultures were harvested by centrifugation and were resuspended on TE buffer for genomic DNA extraction using a Wizard Genomic DNA Purification Kit (Promega).