GSM6333161: BLA, ER, biol rep 1; Rattus norvegicus; RNA-Seq
RNA was harvested using RNA Mini kits kits (Qiagen). ~300ng of total RNA (which contained equal representation from 10 animals' RNA samples) was used for the construction of sequencing libraries.  Samples underwent polyA RNA isolation using the NEBNext Poly(A) mRNA Magnetic Isolation Module prior to library preparation. RNA libraries for RNA-seq were prepared using the NEBNext Ultra Directional RNA Library prep kit following manufacturer's protocols.