RNA-Seq of tachyzoties of toxoplasma gondii
Parasites were allowed to invade and replicate inside host cells for 48 h under standard conditions, Total RNA was extracted from the parasites using the TRIzol Reagent according the manufacturers instructions (Invitrogen, USA) and genomic DNA was removed from the preparation using DNase I (TaKara, Japan). Total RNA was extracted from three biological replicates. RNA quality was assessed using the 2100 Bioanalyser (Agilent, USA).