GSM5418125: abnormal_human_embryo_8-2 [8-2_L3_807D73]; Homo sapiens; RNA-Seq
Human embryo single cell RNA-seq libraries were performed as previously described with minor modifications (Picelli et al., 2014, Macaulay et al., 2016). Briefly, human blastomeres were picked individually into 2.5 μl RLT plus buffer (Qiagen, catalog. no. 1053393). RNA was captured on streptomycin C1 beads conjugated with Biotinylated Oligo-dT30VN primer and the remaining DNA supernatant was collected for HAT-seq. The volume of reverse transcription was adjusted to 10 μl. cDNA was generated according to the smart-seq2 protocol(Picelli et al., 2014). Sequencing libraries were generated and indexed from 1ng of cDNA per sample using the TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme, catalog. no. TD503) according to manufacturer's instructions. Samples were pooled and sequenced using paired-end sequencing on the Illumina Novaseq platform.