GSM5823777: TGF 1; Mus musculus; RNA-Seq
Primary isolated cells were cultured until 80% confluency, treated with 0.1M 4-SU at 20 and 4 hrs prior to crosslinking +/- TGFbeta, cross-linked at 200000 uJ/sq.cm using 368nm light, and total RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols