GSM5550908: Macrophages, Sp rep. 3; Mus musculus; RNA-Seq
Total RNA from cellular lysate was extracted using the NucleoSpin® RNA kit (Macherey‑Nagel, Hoerdt, Germany). Libraries were generated from 1µg of total RNA using Truseq Stranded mRNA kit (Illumina). Libraries were then quantified with KAPA library quantification kit (Kapa biosystems) and pooled. 4nM of this pool were loaded on a Nextseq 500 high output flowcell and sequenced on a NextSeq 500 platform (Illumina) with 2×75bp paired-end chemistry.