GSM5048998: HCT-116- mock -replicate 3 [PAR_3_BC]; Homo sapiens; RNA-Seq
Cells were harvested by trypsinization and total RNA was extracted using TriZol reagent Total RNA depleted of Ribosomal RNA using Ribozero was converted to cDNA and single read 75 bp library was sequenced on Illumina Hi-Seq instrument (IGM core UCSD) RNA-Seq depleted of rRNA using ribozero kit