GSM5352344: STING shRNA 1, 2'3'-cGAMP 1; Nematostella vectensis; RNA-Seq
For polyps, total RNA was extracted using Qiagen RNeasy Mini kits according to the manufacturer's protocol. Embryos were homogenized in Trizol (Thermo Fisher Scientific) and RNA was extracted according to the manufacturer's protocol For polyps, libraries were prepared using WaferGen PrepX library prep kits with oligo dT beads for mRNA enrichment according to the manufacturer's protocol. For embryos, libraries were prepared as follows: oligo dT beads from the KAPA mRNA Capture Kit (KK8581) were used for mRNA enrichment; fragmentation, adapter ligation and cDNA synthesis were performed using the KAPA RNA HyperPrep kit (KK8540); libraries were pooled evenly by molarity and sequenced to generate 25M read pairs per sample