GSM5049882: E4_MII_Cnot6l_Het_smart_rep1; Mus musculus; RNA-Seq
Oocytes were collected from 4-5 weeks old Cnot6l-/- and Cnot6l+/- female mice. The MI oocytes and MII oocytes were similarly collected as described above. Due the limited number of Cnot6l+/- mice, we used C57BL/6 mice as control for Ribo-seq of FGOs, also as control for mRNA-seq of FGOs. Due to data quality, we used MI oocytes Ribo-seq rep12 as control. The RNA-seq libraries were generated using the Smart-seq2 protocol as described previously