DNAseq of Escherichia coli K12 W3110
For DNA preparation, cells were grown over night on solid LB media. Total DNA of the isolates was extracted by QIAmp DNA Mini Kit (Qiagen, Hilden, Germany) according to the manufacturers protocol and used to create Whole Genome Sequencing (WGS) libraries using the Illumina-compatible Nextera DNA Sample Prep Kit (Epicentre, WI, U.S.A.) according to the manufacturer's protocol. The library was sequenced on an Illumina MiSeq sequencer in a 2 x 250 bp run.