GSM6245826: TRV:GhSP-GhCLV2 biol rep2; Gossypium hirsutum; RNA-Seq
RNA was isolated by hot borate (Wan & Wilkins, 1994) followed by column clean-up (Zymo Research, Irvine, CA, USA). Total RNA concentration was determined by nanodrop. 20 ng was used to prepare libraries with NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina. Library quality was assessed at the University of North Texas Health Science Center Genomics Core Facility (Fort Worth, TX, USA) by Agilent High Sensitivity D5000 Screen Tape assay (Agilent Technologies, Santa Clara, CA, USA). Libraries with concentrations ≥ 2 nM were sequenced for 75 cycles on an Illumina NextSeq 550 Sequencer (University of North Texas Health Science Center Genomics Core Facility).