GSM5292862: Sample-33 (snATAC-seq); Homo sapiens; ATAC-seq
Single nucleus suspensions were isolated from about 50mg frozen human prefrontal cortex according to the 10x Genomics Nuclei Isolation with an additional sucrose purification step. Libraries were prepared using 10x genomicsChromium Single Cell ATAC platform (V1) For snRNA-seq and snATAC-seq, single nucleus suspensions were isolated from about 50mg frozen human prefrontal cortex following 10x genomics recommendation. For bulk RNA-seq, total RNA was isolated using Mini Nucleospin RNA kit, Takarabio Libraries were prepared using 10x genomics Chromium Single Cell ATAC platform (V1), 10x genomics chromium V3 for snRNA-seq and SMARTer Stranded Total RNA Sample Prep kit-HI Mammalian  for bulk RNA-seq