GSM5395983: Smoke Month1 4; Mus musculus; RNA-Seq
Intravital labeling of VE-cadherin was performed before mice sacrificed to label the endothelial compartment.  Lung tissue was harvested and enzymatically digested into single cell suspension.  Endothelail cells were  isolated by fluorescence-activated cell sorting and subjected to RNA extraction using Trizol and RNA-easy microRNA kit from Qiagen RNA-seq libraries of polyadenylated RNA were prepared using the TruSeq RNA Library Prep Kit v2 (Illumina) or TruSeq Stranded mRNA Library Prep Kit (Illumina) according to the manufacturer's instructions.