Precision run-on sequencing of Oryza sativa Japonica
Nuclei isolation performed according to the protocol in Hetzel et al: Nascent RNA sequencing reveals distinct features in plant transcription. Proc. Natl. Acad. Sci. (2016). doi:10.1073/pnas.1603217113. Precision run-on sequencing undertaken according to the protocol in Mahat et al: Base-pair-resolution genome-wide mapping of active RNA polymerases using precision nuclear run-on (PRO-seq). (2016). Nature protocols, 11(8), 1455.