GSM5667129: [ATAC-Seq] Human brain pericytes_T4 replicate; Homo sapiens; ATAC-seq
50,000 nuclei from control and transdifferentiating pericytes were used for ATAC-seq with a slight modification. Due to rapid pace of TD and a requirement for sampling at short intervals (2 min), cell lysis and crude nuclei preparation were done on culture plates and without harvesting the adherent cells. Transposition Reaction, Purification, and PCR amplification were performed as per method developed by Buenrostro et al (Curr Protoc Mol Biol 2015). Libraries were prepared using Illumina Tagment DNA TDE1 Enzyme and Buffer Kits.