PacBio Iso-Seq full-length non-chimeric transcriptome data of Drosohphila melanogaster ARL0003_adult_ovaries, 2-3 kb fraction
Iso-Seq libraries from each size fraction were run on a Pacific Biosciences RS II using P6 polymerase, DNA Sequencing Reagent 4.0 v2, and a movie collection time of 240 minutes. Iso-Seq library reads were converted to circular consensus sequence (CCS) reads and analyzed with SMRTLink v7.0 Iso-Seq analysis pipeline to identify full-length non-chimeric CCS reads with single end primer sequences and poly-A tail.
Iso-Seq libraries were created using Pacific Biosciences protocol for the Clontech SMARTer PCR cDNA Synthesis Kit and manual agarose gel size-selection.  Three libraries were prepared from each sample using 33 ng total RNA per library. The size ranges for the three libraries were approximately 0.8-2 kb, 2-3 kb, and 3-5 kb. SRMTbell libraries were created with Pacific Biosciences SMRTbell DNA Template Prep Kit V1.0.