GSM4781255: HEK293T polyA RNA MgR-rep2; Homo sapiens; RNA-Seq
RNA was collected using RNAzol1, and polyA+ RNA was purified by Oligo d(T)25 Magnetic Beads (New England Biolabs) following the manufacturer's instructions. Total RNA or polyA+ RNA was chemically fragmented in to 20-200-nucleotides-long by RNA Fragmentation Reagents (Thermo Fisher Scientific). After purification and 3' end repairation, the RNA was subjected to MgR digestion and followed by Oxidation-Elimination-Dephosphorylation treatment. The remaining RNA was 5'end repaired and  then prepared for sequencing using small RNA library protocols except using the  high-definition 3'- and 5'- adaptors.