GSM5048990: Pooled_5geno_6tp_3rep_3000-5000nt_fract; Hordeum vulgare; Blumeria graminis f. sp. hordei; OTHER
Total RNA was isolated from 0.5 g of each pool using a hot (60˚C) phenol / guanidine thiocyanate method (Caldo et al., 2004). The pooled sample (90 experimental units) was SAGE-ELF size selected to generate 6 PacBio cDNA Iso‐Seq Library Preparations [fragment sizes: 1) non-fractionated 2) 1000-1500 nt 3) 1500-2000 nt 4) 2000-3000 nt 5) 3000-5000 nt 6) > 5000 nt].  The non-fractionated as well as the three smallest fragment libraries were each loaded onto 3 SMRT cells, and the two largest fragment libraries were each loaded onto 2 SMRT cells for a total of 16 SMRT cells.