WGS of EXP1 (no bottleneck)
A genomic DNA sample of each population was subjected to paired-ends Illumina sequencing at 2  150 bp read length and 350 bp insert size. A total amount of 1 g of DNA per sample was used as input material for the DNA sample preparation. Sequencing libraries were generated using a NEBNext Ultra DNA Library Prep Kit for Illumina following the manufacturers protocol and index codes were added to attribute sequences to each sample. The whole genome of ancestral WT population and evolved populations were sequenced using Illumina NovaSeq 6000.