DNA-Seq of Bos taurus: adult male
The genomic DNA was fragmented by acoustic shearing with a Covaris S220 instrument. Fragmented DNA was cleaned up and end repaired. Adapters were ligated after adenylation of the 3ends followed by enrichment by limited cycle PCR. The DNA library was validated using a High Sensitivity D1000 ScreenTape on the Agilent TapeStation (Agilent Technologies, Palo Alto, CA, USA), and was quantified using Qubit 2.0 Fluorometer. The DNA library was also quantified by real time PCR (Applied Biosystems, Carlsbad, CA, USA). The sequencing library was clustered onto a flowcell. After clustering, the flowcell was loaded onto the Illumina instrument according to manufacturers instructions. Image analysis and base calling were conducted by the Illumina Control Software. Raw sequence data (.bcl files) generated from the Illumina instrument was converted into fastq files using Illumina bcl2fastq 2.17 software. One mismatch was allowed for index sequence identification.