GSM4879586: HFA571: Fetal single cell RNA sequencing material; Homo sapiens; RNA-Seq
Single cells were obtained following mechanical and enzymatic (trypsin) dissociation as previously described (Cui et al. 2017). A isotonic Percoll gradient was used to remove myelin. The whole dissociated cell population was collected from the gradient (which sits between the myelin layer and the red blood cell layer), washed 3 times in PBS, and re-suspended at a concentration of 10^6 cells per ml. Fetal cell isolation does not require the percoll gradient step. Made using the GemCode Single-Cell Instrument (10x Genomics) and Single Cell 3' Library & Gel Bead Kit v2 and Chip Kit (10x Genomics)