GSM5698658: Untreated_MMTV_Wnt1_scRNA-seq; Mus musculus; RNA-Seq
Single cell suspensions of mammary tumors were generated, assessed for viability, and transcriptomes generated using 10X Chromium single cell platform. To generate scRNA-seq libraries, barcoded single-cell gel beads in emulsion were made and reverse transcribed according to manufacturer instructions (Chromium Single Cell 3' Reagents Kit, 10X Genomics).