RNA-Seq of mus musculus: MMTV Nmi knockout mammary tumor
RNA was isolated from mammary tumors on MMTV-Neu Nmi wild-type and knockout mice, using the RNAeasy Mini kit as per the manufacturers protocol. Next Gen-Sequencing was performed by GENEWIZ Inc. (Chelmsford, MA). RNA library preparation was performed following rRNA depletion using a polyA selection method. RNA sequencing was performed using the Illumina HiSeq system in a 2  150-bp configuration 350M PE reads single index per lane. (GENEWIZ, US).