GSM5049679: S30_12Sham_3; Xenopus laevis; RNA-Seq
RNA was extracted by homogenizing all individual embryos in 600 ul TRI reagent (Ambion) then dividing the homogenate equally between two tubes. Partially separated mixture was spun down in a Phase Lock Gel Heavy tube (QuantaBio) and RNA was then purified using the MagMAX™- 96 for Microarrays. RNA quality and yield was assessed by spectrophotometry (NanoDrop®), agarose gel electrophoresis, and Agilent Bioanalyzer analysis. RNA was extracted from single embryos using the  MagMAX™-96 Total RNA Isolation Kit. RNA libraries were prepared for sequencing using standard Illumina protocols