mNGS:respiratroy tract
Total DNA was extracted with Maxwell RSC Viral Total Nucleic Acid Purification Kit (Promega, USA) after pretreatment with lysozyme (Sangon Biothech, Shanghai, China) and lyticase (TIANGEN Biothech, Beijing, China), and fragmented into 200 bp to 300 bp fragments with a Bioruptor Pico. DNA libraries were constructed with VAHTS Universal Plus DNA Library Prep Kit for Illumina (Vazyme, Nanjing, China).