GSM6245671: RNA-seq XEN cells, Rad21-degron, dTAG treated, clone2, rep2; Mus musculus; RNA-Seq
Cells were lysed in lysis buffer (0.2% (vol/vol) Triton X-100 and 2 U/μl RNase inhibitor). The polyadenylated mRNAs were captured by the oligodT primers, RNA-seq (Smart-seq2): mRNAs were subjected to the Smart-seq2 protocol as described previously (Picelli et al., 2014).