WES of human: child female peripheral blood
The 300ng genomic DNA concentrations were sheared with Covaris LE220 Sonicator (Covaris) to target of 150-200bp average size. DNA libraries were prepared using SureselectXT Low Input reagent kit (Agilent). The fragments were repaired the 3and 5overhangs using End repair mix (component of SureselectXT) and purified using Agencourt AMPure XP Beads (Beckman). The purified fragments were added with A tail using A tailing Mix (component of SureSelectXT) and then ligated with adapter using the DNA ligase (component of SureselectXT). The adapter-ligated DNA fragments were amplified with Herculase II Fusion DNA Polymerase (Agilent). Finally, the pre-capture libraries containing exome sequences were captured using SureSelect Human All Exon V5 kit (Agilent).