RNA-Seq of Homo Sapiens: Normal glucose Neutrophils
Adapter in RNA sequences were trimmed using seqan/Flexbar package. The reads were then aligned using bwa-mem with GRCh38 was used as reference genome and bam files were built. Sequences were then assembled by Rsubread package.  In order to map cDNA fragments obtained from RNA sequencing GRCh38 was used as reference genome. Gene expression  analysis was performed using edgeR/limma package.