Illumina HiSeq 4000 sequencing; 3' UTR RNA-sequencing of syngenic EGFR-mutant mouse tumor transplants
3' UTR RNA-sequencing of syngenic EGFR-mutant mouse tumor transplants
Mice were treated with osimertinib or vehicle p.o. for indicated duration. Tumors were harvested and RNA was extracted using RNeasy Mini kit. Sequencing libraries were prepared using the QuantSeq 3' mRNA-Seq library kit (Lexogen, Austria)