GSM4729889: HEK293T_WT_re1; Homo sapiens; RNA-Seq
RNA was isolated from transfected cells using the RNeasy Plus mini kit (Qiagen, #74136) following manufacturer's instructions. RNA-seq library construction using the TruSeq Stranded Total RNA Gold (Illumina, RS-122-2303). The qualities of RNA-seq libraries were verified using the Tape Station D1000 assay (Tape Station 2200, Agilent Technologies) and the quantities of RNA-seq libraries were checked again using real time PCR (QuantStudio 6 Flex Real time PCR System, Applied Biosystem). Libraries were normalized and pooled and then 75-bp paired-end reads were sequenced on the Hiseq 3000 platform (Illumina).