WGS of Heliothis subflexa single pupa (PE_1)
Heliothis subflexa from a laboratory colony reared at North Carolina State University were sib-mated for four generations. This laboratory population was established from a field collection made in Orangeburg County, South Carolina, in 1997. Genomic DNA was isolated from one male progeny from this fourth generation of inbreeding using a Qiagen DNeasy kit (Qiagen, Valencia, CA, USA) using a modified mouse tail protocol. DNA from this individual was sent to the North Carolina State University Genomic Sciences Laboratory (NCSU-GSL; Raleigh, NC, USA) for Illumina TruSeq Library preparation and sequencing on a single lane of the Illumina HiSeq2500 in rapid run mode. We prepared genomic DNA from a second male pupa from the same colony, but which was not inbred using a similar approach. DNA from this second pupa was submitted to the Michigan State University Research and Technology Support Facility (East Lansing, MI, USA) for Illumina 2-kb and 5-kb mate pair (MP) library preparation. The MP library was prepared using an Illumina Nextera Mate Pair Sample Preparation Kit according to standard protocol, and validated using a Qubit dsDNA assay, Caliper LabChipGX (PerkinElmer, Waltham, MA, USA) and Kapa Library Quantification qPCR for Illumina Libraries. The library was sequenced on one lane of an Illumina HiSeq 2500 at the NCSU-GSL.