16S rRNA gene amplicon sequencing of surrounding water
Library preparation and amplicon sequencing was performed at the University of Illinois at Chicago DNA Services Facility, USA. The 16S primer sets (CS1-341F and CS2-806R primers) were used to amplify the hypervariable V3-V4 region of bacterial 16S genes. The amplicon sequences was generated using Illumina MiSeq platform with the sequencing strategy of 300 bp paired-end employing V3 chemistry for 16S rRNA genes.