GSM4824455: 160; Homo sapiens; RNA-Seq
RNA was extracted using TriZOL and Direct-zol RNA extration columns (Zymo Research). RNA (ug) was digested with Rnase R (2U/ug RNA; Epicentre) for 30mins at 37oC, then purified by Rneasy MinElute Cleanup kit (Qiagen). Libraries were constructed with RNase R-treated RNA using the TruSeq Stranded Total RNA library preparation kit (with Ribo-zero rRNA depletion module).