GSM5396293: Vehicle control 1; Homo sapiens; RNA-Seq
Cells were harvested and total RNA was extracted using RNeasy Mini Kit. The purity of RNA (RNA integrity number or RIN) was assessed using 2100 Bioanalyzer instrument (Agilent, CA). To prepare sequencing libraries from mRNA we used TruSeq Stranded mRNA library prep kit (Illumina, CA).   Sequencing was performed on NextSeq Hi (Illumnina, CA) sequencing instrument with setting at following output: R1=100; index=8; R2=100.