GSM4780604: scTCR-seq of mus musculus: tumor infiltrating immune cells [MOC1_scTCR_ctrl]; Mus musculus; RNA-Seq
Total RNA was extracted using RNeasy Plus Mini kit (QIAGEN) according to manufacturer's protocol. Genomic DNA was isolated using the DNeasy Blood and Tissue Kit (Qiagen) WES Library construction was performed using the Agilent Sure Select Whole Exome Capture and captured using the Agilent SureSelect XT Mouse All Exon probe sets according to the manufacturer protocol.For bulk RNAseq, standard mRNA library preparation kit (RS-122-2101, Illumina) was used for library preparation. The single-cell RNA-seq libraries were processed using ChromiumTM single-cell 5' library & gel bead kit and coupled TCR-seq libraries were obtained using ChromiumTM single-cell V(D)J enrichment kit (10x Genomics).