GSM5418886: AHR KO_Stem cells_DMSO-2; Mus musculus; RNA-Seq
Colon crypts were isloated with 2mM EDTA wash. Crypts embedded in matrigel were cultured in stem cell enrichment media containing Wnt-3A, Noggin, Rspondin-1 and EGF. RNA was extracted from organoids and quantified with Bioanalyzer RNA libraries were prepared for sequencing using standard Illumina protocols