GSM5650999: Untreated-H3K27ac_Rep1_in1; Mus musculus; ChIP-Seq
For ChIPseq data, 10 million cells were crosslinked, lysed, sonicated, and chromatin was immunoprecipitated with 10ug (ELF1, CEBPA) or  2ug (histone marks) antibody. Immunoprecipitations were reversecrosslinked and DNA purified with AmPure XP beads was used for library preparation. For CUT&RUN, 0.5 million cells were collected and incubated with a 1:100 dilution of antibody. pAG Mnase was used at 1:20 dilution and isolated DNA used for library generation. NEBNext Ultra II DNA Library Prep Kit (E7645) with NEBNext Multiplex Oligos (E6440) with optimized PCR extension time and number of cycles.