GSM5463593: 6.HCT116.KO.shGFP.rep2; Homo sapiens; RNA-Seq
The RNA of the treated cells was extracted by TRIzol (Life Technologies, 87804) according to the manufacturer's protocol. Total RNA was made into libraries for sequencing using the mRNA-Seq Sample Preparation Kit (Illumina) and sequenced on an Illumina Hiseq platform (Novagene, Tianjin, China), which includes that mRNA was purified from total RNA using poly-T oligo-attached magnetic beads.