NextSeq 500 sequencing; ZXDA/B dependent transcription in HCT116 human colon carcinoma cells
ZXDA/B dependent transcription in HCT116 human colon carcinoma cells
Cells were collected 48 hours after transfection and lysed in RLT buffer. HCT116 cell line was transfected (RNAiMAX) using two different control and ZXDA/B targeting siRNA. RNA was extracted using QIAGEN Rneasy plus kit. Libraries were prepared using NEBNext Ultra II RNA Library Prep Kit.