GSM5048543: ChIP-Seq Person living with HIV sample 21, Alveolar macrophage, Mycobacterium tuberculosis challenged library; Homo sapiens; ChIP-Seq
Cells were lysed with Trizol and kept at -80°C until extraction.  For ChIP-seq, AM were crosslinked with 1% w/v formaldehyde for 10 min and then sonicated to 150-500 bp using a S220 (Covaris). ChIP-DNA was prepared using a manual chromatin immunoprecipitation method with an Antibody-Antigen for H3K27ac (Abcam, Ab4729, GR3211959-1). incubation of 18 hrs., followed by bead incubation for 135 minutes, and 6 x 5-minutes washing steps. ATACseq libraries were prepared for sequencing using standard single end sequencing.