GSM4781078: camt-1::gfp; egl-19(n582) replicate 1 pulldown; Caenorhabditis elegans; ChIP-Seq
Worms were snap-frozen in liquid N2, hand ground, fixed in formaldehyde and sonicated. CAMT-1::GFP pulldown was performed using Abcam anti-GFP antibodies and the DNA were de-crosslinked overnight at 65 degree Celcius. Libraries were prepared following the protocol from NEBNext Ultra II DNA Library Prep Kit for Illumina.