GSM5823860: eif4a3KD-CtrlOE, rep2; Mus musculus; RNA-Seq
RNA was extracted with the Rneasy Plus kit (Qiagen, #74136) according to the manufacturer's instructions. For ESCs, 1 µg total RNA from each sample was taken for the preparation of PolyA RNA-seq libraries, and massively parallel sequencing was performed with the HiSeq platform. Single-end 100 bp-length reads or paired-end 150 bp-length reads were produced.