Bulk RNA-seq of CAMs from murine PBMCs
Peripheral blood samples from 10- to 16-week-old C57BL/6J (B6) mice (n=60, 1 ml per mouse) were collected in EDTA-coated tubes, pooled according to the experimental requirements. The CD11b-selected monocyte suspension was cultured for 6 or 7 days with RPMI/FBS plus 30 ng/ml M-CSF. For activation in vitro, mouse differentiated macrophages were stimulated with lipopolysaccharide (LPS, 500 ng/ml) and IFN (20 ng/ml) to M(LPS/IFN)s, which were classically activated macrophages (CAMs).