Illumina HiSeq 4000 sequencing; 3' UTR RNA-sequencing of oncogene-driven human cancer cell lines treated with a kinase inhibitor versus controls
3' UTR RNA-sequencing of oncogene-driven human cancer cell lines treated with a kinase inhibitor versus controls
Cells were treated for 72h as indicated and then harvested by trypsination. RNA was extracted using Qiagen RNeasy kits. Sequencing libraries were prepared using the QuantSeq 3' mRNA-Seq library kit (Lexogen, Austria)