RNA-Seq of F11 Dif 2
Twenty million F11 cells per dish were seeded in Corning square bioassay dishes (CLS43110; SigmaAldrich), and 24 hours later, the culture medium was replaced with differentiation medium. After 72 hours, total RNA from F11 cells was extracted and purified employing the RNeasy Mini Kit (Qiagen, Hilden, Germany). RNA quantity, integrity and quality were estimated using a 2100 Agilent Bioanalyser (Agilent Technologies, Las Rozas, Madrid, Spain) to calculate RIN (RNA integrity number). The RIN value of all the samples was greater than 8. A NextSeq 500 platform (Illumina, San Diego, CA, USA) was employed to sequence the transcriptome of F11 cells. Three RNA sequencing (RNA-Seq) experiments were performed using independent samples.