GSM5418613: L1iT05; Gallus gallus; RNA-Seq
The total RNA of the corresponding four intestinal tissues was prepared for mRNA sequencing using TRizol reagent (Invitrogen, CA, USA) following the manufacturer's protocol at Novogene Bioinformatics Technology Co., Ltd. The RNA integrity and yield were assessed by the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA) and the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA), respectively. The RNA Integrity Number (RIN) values were all greater than 6.8 (average of 8.28), and the RNA yield of each sample was more than 4 μg. In accordance with the manufacturer's protocol of the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, MA, USA), 3 μg of RNA per sample was used to generate sequencing libraries with index codes added to assign sequences to each sample. The libraries were clustered following the manufacturer's recommendations of the cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumina Inc.). Finally, the clustered libraries were sequenced on an Illumina HiSeq platform (by Novogene Bioinformatics Technology Co., Ltd.) and 150 bp paired-end reads were generated.