GSM5584631: MDA-MB-453_PolII_ChIP_D11-8_2; Homo sapiens; ChIP-Seq
MDA-MB-453 cells were fixed with 1% formaldehyde for 10 min prior to quenching. Chromatin was prepared and subsequently sheared as previously described (Mohammed et al. 2015, Nature). Sheared chromatin was immunoprecipitated with 10 µg of RNA Polymerase II (Pol II) antibody (Santa Cruz, SC-47701), after coupling to Protein A Dynabeads (10002D). Recovered DNA was amplified using the NEBNext Ultra IIDNA Library Prep Kit (NEB) following manufacturer's protocols. DNA libraries were sequenced on the Illumina Novaseq 6000 to a minimumof 60 million 150 bp paired-end reads per sample.