GSM6245848: Erythroblasts_Hic_Rep2; Gallus gallus; Hi-C
10 x 106 cells were fixed with formaldehyde 2% for 10 minutes at room temperature. Chromatin was digested with MboI overnight and 5′ overhangs were filled with Biotin-14-dATP and religated in nuclei. DNA fragments were purified and shared to an average size of 500 bp using a Covaris machine. Hi-C libraries were generated using the TruSeq Illumina system.