GSM5395231: SSRP1 KO rep2; Mus musculus; ChIP-Seq
ChIP samples were eluted from beads with elution buffer containing 1% SDS at 65°C for 20 min and were purified by phenol-chloroform extraction and ethanol precipitation in parallel with input samples. For sequencing, ChIP libraries were prepared using MicroPlex Library Preparation Kit v2 (Diagenode) according to manufacturer's instructions. Each library was quantified on Qubit with Qubit® dsDNA HS Assay Kit (Life Technologies) and size distribution was examined on the Fragment Analyzer with High Sensitivity NGS Fragment Analysis kit (Agilent). Libraries were then sequenced on a High-output flow cell (400M clusters) using the NextSeq® 500 High Output v2.5 150 cycles kit (Illumina), in paired-end 75/75nt mode, according to manufacturer's instructions at the TGML Platform of Aix-Marseille Université.