GSM4779262: HCC213L_ctrl_CD4; Homo sapiens; RNA-Seq
2 TIL subsets were sorted according to the expression of CD45/CD3/CD4/CD8. CD8+ (CD45+CD3+CD8+CD4-) TILs and CD4+ (CD45+CD3+CD4+CD8-) TILs were sorted directly into RLT+ lysis buffer. RNA was immediately isolated using RNeasy micro kit (Qiagen) according to manufacturer's instructions Barcoded mRNA-seq cDNA libraries were prepared using TruSeq RNA Access Libraryprep Kit (Illumina) according to the manual Barcoded RNA-Seq libraries were onboard clustered using HiSeq X Kit v2 using 2.5 nM and PE 150 bps and were sequenced on the Illumina HiSeqX using the HiSeq X Kit v2 (PE 150 cycle)