GSM5552034: Post injury with FAK inhibitor day 14 3; Mus musculus; RNA-Seq
Mice were treated with or without FAK-I for 2 weeks after wire injury. Femoral arteries were isolated, and the fat and adventitia layers were carefully removed under surgical microscope. To remove endothelial and immune cells, the artery lumen was quickly flushed with 150 µl TRIzol lysis reagent (Invitrogen) using a 29-gauge insulin syringe. Media were frozen in liquid nitrogen and lysed in 500 µl TRIzol  per artery, and total RNA was isolated using the RNeasy column. RNA libraries were prepared for sequencing using standard Illumina protocols