GSM4879753: KO36_C5; Homo sapiens; RNA-Seq
lysed in 100Sµl lysis buffer containing TCL buffer (Qiagen, 1031576) containing 1% β-mercaptoethanol. After lysis, plates were imminently frozen and stored in -80C until samples were processed. For RNA purification 10µl of cell lysates for each sample was used. 40µl of Agencourt RNAClean XP SPRI beads (Beckman Coulter, A63987) were added to the cell lysates, and after 10 minutes of incubation in room temperature, plates were place on a DynaMag 96 side skirted magnet (Invitrogen, 12027) for 5 minutes, followed by supernatant removal and 2 cycles of 75% ethanol washes RNA libraries were prepared for sequencing using standard Illumina protocols