TAD-seq of presort cells expressing TAD-PYL1 after 48 hours of ABA treatment
Performed nested PCR on the purified genomic DNA using primers targeting T7 promoter and PYL1 of the backbone vector in the first step creating a ~470 bp product. Products of the first reaction were then pooled and amplified for an additional 10 steps using primers targeting outside the Gateway sites creating a ~300 bp product. Libraries were quantified on Qubit dsDNA Broad Range kit and paired-end sequenced on an Illumina MIseq with a custom PAGE-purified R1 sequencing primer.