GSM5352556: WT_30_MG_19; Saccharomyces cerevisiae; RNA-Seq
Total RNA was extracted using standard hot phenol chloroform extraction. A fixed quantity of total RNA plus a fixed quantity of 4-thiouracil labeled total s. pombe RNA were combined for each sample. 4-thiouracil labeled RNA was purified via biotinylation and pull-down with streptavidin beads. Libraries were constructed via multiplexed primer extension sequencing (MPE-seq)