RNA-Seq of iRECs
RNAseq libraries were prepared starting from 1g of total RNA using the Universal Plus mRNA-Seq kit (Nugen) as recommended by the manufacturer. The oriented cDNAs produced from the poly-A+ fraction were sequenced on a NovaSeq6000 from Illumina (Paired-End reads 100 bases + 100 bases). A total of 50 millions of passing filter paired-end reads was produced per library.