WES of Homo sapiens: adult  infertility male blood
Genomic DNA was isolated from peripheral blood samples of proband using whole blood DNA purification kits (Qiagen GmbH). We captured exons by WES using Agilent SureSelect Human All Exon V6 Kits (Agilent Technologies) and an Illumina HiSeq 4000 sequencer (Illumina). Variants were prioritized and etiological involvement was evaluated using the medical resequencing analysis pipeline (MERAP), which initially filters all identified variants by comparisons with disease-associated variants in the Human Gene Mutation Database (HGMD) and the Online Mendelian Inheritance in Man (OMIM), to collect known disease-causing variants. Pathogenic and likely pathogenic genes/variants were defined according to the standards and guidelines of American College of Medical Genetics and Genomics.