GSM6246290: ΔwatR strain after 10 min of acetate treatment, biol rep 2; Methylomonas sp. DH-1; RNA-Seq
Total RNA was extracted by RNeasy Mini kit (#74106, Qiagen) according to the manufacturer's instructions. 1 ug of total RNA was proceeded to rRNA depletion using NEBNext rRNA depletion kit (Bacteria) (#7850, NEB). Resulted mRNA was used for sequencing library construction by TruSeq Stranded mRNA Library Prep kit (#20020594, Illumina). All experiments were performed following manufacturer's instructions.