GSM5050306: InP/ZnS QD treated yeast rep3; Saccharomyces cerevisiae; RNA-Seq
TruSeq Stranded mRNA LT Sample Preparation Kit (Illumina, San Diego, CA) was used to isolate mRNA from the total RNA. The kit provided SuperScript II Reverse Transcriptase that was used to synthesize the first cDNA strands from the newly purified mRNA, followed promptly by the synthesis of the second cDNA strand. Using unique adaptors ligated to each cDNA sample, the samples could be distinguished when sequenced and then amplified on both ends for 15 cycles in a T100TM Thermal Cycler (BIO RAD). The cDNA of each sample was suspended in 30 mL of Resuspension Buffer with a concentration of approximately 50 ng/mL.