GSM6276590: Matthias, IgG; Mus musculus; RNA-Seq
P6 lungs were perfused with PBS through the RV, minced with a razor blade, pooled (n=3 per condition), and digested  in 2.4 U/mL Dispase II/0.1% collagenase A in 150 mM NaCl, 10 mM Hepes pH 7.4, 2 mM CaCl2 for 45 min at 37C. Enzymes were inactivated with 10 mM EDTA and cells were passed through 70-um and 40-um filters. Spun-down cell pellets were washed twice and resuspended in 0.5% BSA-PBS. Life cell numbers were counted (Trypan Blue dye). Single cells (~20,000/sample) were loaded onto a GEM code instrument (10xGenomics). GEM generation and library prep was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics).