GSM5697085: S348_2: MCV LT 2; Homo sapiens; RNA-Seq
Cells expressing empty vector, MCV LT, MCV dMUR were trypsinized and harvested at day 14. p21 knockdown cells were trypsinized and harvested at day 21. Total RNA-Seq libraries were constructed from 300 ng of total RNA. To remove rRNA, a method described by Bogdanova et al. (2011) was followed with some modifications. Libraries were prepared using the Zymo-Seq RiboFree Total RNA Library Prep Kit (Cat # R3000) according to the manufacturer's instructions (https://www.zymoresearch.com/products/zymo-seq-ribofree-total-rna-library-kit). RNASeq libraries were sequenced on an Illumina NovaSeq to a sequencing depth of at least 30 million read pairs (150 bp paired-end sequencing) per sample.