GSM6246312: Overexpressed EBF4 ATAC-seq rep1; Homo sapiens; ATAC-seq
We followed the Omni-ATAC protocol. Briefly, 50000 cells were washed and lysed in lysis buffer (10 mM Tris-HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl2) containing 0.1% NP40, 0.1% Tween-20, and 0.01% Digitonin for 10 min on ice to prepare the nuclei. Immediately after lysis, nuclei were spun at 500 x g for 10 min to remove the supernatant. Nuclei were then incubated with Tn5 transposon and tagmentation buffer (20 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 20% Dimethylformamide) containing 0.1% Tween-20 and 0.005% Digitonin at 37 °C for 30 min. Stop buffer was added directly into the reaction to end tagmentation. PCR was performed to amplify the library. Libraries were then purified with SPRI (Beckman) beads and deep sequencing was performed.