Transcriptomic data of Dendrolimus kikuchii from head of female adult using BGISEQ sequencing
After the total RNA of the sample is extracted, the mRNA is enriched with magnetic beads with oligo (DT). Fragment buffer was added to break the mRNA into short fragments. Using mRNA as template, the first cDNA strand was synthesized with six base random primers. After purification by qiquick PCR kit, terminal repair was performed. Then, agarose gel electrophoresis was used to select the size of the fragments. Finally, PCR was amplified. The sequenced library was constructed with BGISEQ Sequencing.