GSM5584165: MIS-C patient 7 / Sibling 1 MIS-C patient 5; Homo sapiens; RNA-Seq
After thawing PBMCs were counted, isolated and spun down.  The cell pellet was resuspended and incubated for 30 min on ice with staining mix in PBS containing 0.04% BSA, L/D-DAPI, CD3-FITC (HIT3A), CD14-PerCP-Cy5-5 (HIB19), CCR5-PE (2D7), Human TruStain FcX (BioLegend, Cat. 422302) and TotalSeq-C hashing antibodies (BioLegend). The sorted single-cell suspensions were resuspended and loaded on a Chromium GemCode Single Cell Instrument (10x Genomics) to generate single-cell gel beads-in-emulsion (GEM). The scRNA/Feature Barcoding/TCR libraries were prepared using the GemCode Single Cell 5' Gel Bead and Library kit, version 1.1 (10x Genomics, Cat. 1000165) according to the manufacturer's instructions.