GSM5623238: INSM2_P0P5_overexpression_scRNAseq; Mus musculus; RNA-Seq
Retinal cells were dissociated from explants for fluorescence-activated cell sorting (FACS) as described previously (Hoang et al., 2020). GFP+ cells were collected in ice-cold PBS with 10% heat-inactivated fetal bovine serum (FBS). ScRNA-seq analysis was performed on dissociated retinal cells using 10x Genomics. Briefly, dissociated retinal cells (~10,000 cells per sample) were loaded into a 10x Genomics Chromium Single Cell system using Chromium Single Cell 3' Reagents Kits v3.1 (10X Genomics, Pleasanton, CA). scRNA libraries were generated by following the manufacturer's instructions. Libraries were pooled and sequenced on Illumina NextSeq 500 or NovaSeq 6000. Sequencing reads were processed through the Cell Ranger 3.1 pipeline (10x Genomics) using default parameters.