GSM5005487: iN-VO, 15 Days Induction; Homo sapiens; RNA-Seq
To dissociate organoids for single cell RNA-seq, 5-6 organoids were incubated in a 1 mL 20 U/mL Papain solution (Worthington, LS003126) for 30 minutes, passed through a 40 𝜇m filter, spun down at 300 rcf for 5 minutes, and resuspended in 0.04% BSA solution. Cells were then loaded into the Chromium Chip B (10x Genomics). Libraries were constructed using the standard Chromium Single Cell 3ʹ Reagent Kits v3 workflow (10x Genomics).