RNA-seq of gallus gallus: three-week-age of male AA broiler
Nine RNA samples at each time point were randomly divided into three groups, and three samples in each group were mixed with equal amount of RNA. A total of 3 g for each mixed sample was used as the input material to construct the cDNA library after cleaned up ribosomal RNA (rRNA). Subsequently, sequence libraries were generated using the rRNA-depleted RNA by NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, Beijing, China) following the manufacturers protocol. Finally, after cDNA libraries were purified, assessed and quantified, the libraries were sequenced at Illumina Hiseq 2500 platform. The 150 bp-paired-end raw reads were converted from the original data which processed from this platform via CASAVA Base Calling analysis and stored at FASTQ files.