LINE1 transfected and SARS-CoV-2 infected HEK293T cells
HEK293T cells were obtained from ATCC (CRL-3216) and cultured in DMEM supplemented with 10% heat-inactivated FBS (Hyclone, SH30396.03) and 2mM L-glutamine (MP Biomedicals, IC10180683) following ATCCs method. Plasmid pBS-L1PA1-CH-mneo was a gift from Astrid Roy-Engel (Addgene plasmid # 51288 ; http://n2t.net/addgene:51288 ; RRID:Addgene_51288). Transfection was done with Lipofectamine 3000 (Invitrogen L3000001) following manufacturers protocol.SARS-CoV-2 USA-WA1/2020 (Gen Bank: MN985325.1) was obtained from BEI Resources and expanded and tittered on Vero cells. Cells were infected in DMEM +2% FBS.Cellullar DNA extraction was done using a published method (Lee et al. Nature, 563, 639645(2018)).DNA extracted from HEK293T cells transfected with the pBS-L1PA1-CH-mneo plasmid and infected with SARS-CoV-2 was used to make a sequencing library with the SQK-LSK109 kit (Oxford Nanopore Technologies).