RAD-Seq of the cricket Gryllus campestris
We used the DNeasy Blood & Tissue kit, QIAGEN (Germantown, MD, USA) to extract genomic DNA from each of the 110 individuals. The DNA concentration from each individual was normalized to 20 ng/l in 10 l before double digesting it with Sbf1 and Msp1 (New England Biolabs). Paired-end RAD tag libraries were constructed using the protocol described in Poland et al. (2012). After the final PCR amplification step, we used AMPure Beads for clean-up and size selection to obtain a library size of approximately 200-600 bp. Individual libraries were barcoded and pooled into three libraries that were Illumina sequenced (100 bp paired-end). Raw read processing was done as in Settepani et al. (2017) to obtain data for further analyses.