Sequence reads C311
Genomic DNA was prepared using the GenElute kit (Sigma-Aldrich) and PacBio long-read sequencing was carried out at SNPsaurus (Eugene, OR). SMRTbell libraries were prepared using the Express Template Prep Kit 2.0 from according to the manufacturers protocol (Pacific Biosciences, CA). Samples were pooled into a single multiplexed library and size selected using Sage Sciences BluePippin according to the manufacturers recommendations using the 0.75% DF Marker S1 High-Pass 6 kb-10 kb v3 run protocol and S1 marker. A size selection cut-off of 8000 (BPstart value) was used. The size-selected SMRTbell library for each strain was annealed and bound according to the SMRT Link Set Up, pooled, and sequenced on a Sequel II with 30 hour movie time.