GSM5516758: SK-N-DZ cell line, replicate 2; Homo sapiens; ATAC-seq
Cells were cooled slowly to minimize cell lysis with freezing. Cell pellets were resuspended in lysis buffer, pelleted, and tagmented using the enzyme and buffer provided in the Nextera Library Prep Kit (Illumina) Tagmented DNA (Nextera Library Prep Kit, Illumina) was then purified using the MinElute PCR purification kit (Qiagen), amplified with 10 cycles of PCR, and purified using Agencourt AMPure SPRI beads (Beckman Coulter). Resulting material was quantified using the KAPA Library Quantification Kit for Illumina platforms (KAPA Biosystems), and sequenced with PE42 sequencing on the NextSeq 500 sequencer (Illumina).