GSM5293227: KO; Mus musculus; RNA-Seq
Livers were harvested, snap frozen with liquid nitrogen, and RNA was harvested using RNA STAT-60 reagent (Amsbio). DNase digestion was performed on column with an RNAeasy Kit (Qiagen) according to manufacturer's instructions. RNA concentration was estimated with a NanoDrop (Thermo Fisher). Equal masses of RNA (2.5 ug) from mice of the same genotype were pooled to create one sample per genotype. Library preparation was completed with an Illumina TruSeq Stranded Total RNA kit. RNA libraries were prepared for sequencing using standard Illumina protocols