GSM5462552: Gastric adenocarcinoma cells - AGS KO NAT10-2; Homo sapiens; RNA-Seq
Total RNA was isolated from AGS with or without NAT10 knockout and BGC823 cells expressing shNAT10 or the control shRNA using TRIzol reagent. Poly(A)+ mRNA was subsequently purified from total RNA via Dynabeads mRNA Purification Kit (Invitrogen),and then sequenced on the Illumina NovaSeq 6000 platform. RNA libraries were prepared for sequencing using standard Illumina6000 protocols