GSM5418446: Tnmd Knockout ScxGFP+ cell #7; Mus musculus; RNA-Seq
Uninjured Achilles tendons were enzymatically digested for 8 hours with collagenase, filtered (Alberton et al. 2015) resuspended in PBS, picked based on GFP expression using a micromanipulator and stored in Smart-Seq2 lysis buffer at -80°C). RNA libraries were prepared for sequencing using standard Illumina protocols