WGS of metagenome: a soil sample
Whole metagenome libraries were prepared from 10 ng of soil DNA. DNA was sheared to ~300 bp using the Covaris E220 Focused-ultrasonicator (Covaris) and processed through the Invitrogen Collibri PS DNA Library Prep Kit for Illumina Systems (Thermo Scientific) workflow. Fragment size distribution was then assessed by Agilent Fragment Analyzer system (Agilent Technologies) with HS NGS Fragment kit. Quantification of sequenceable molecules was performed with Invitrogen Collibri Library