RNA-seq microbiome of tongue coating sample in Homo sapiens
Primers targeting the hypervariable V3-V4 region of the 16S rRNA gene were used to amplify the extracted DNA samples. The forward primer was 5'-CCTACGGGNGGCWGCAG-3' and the reverse primer was 5'-GACTACHVGGGTATCTAATCC-3'. AxyPrep PCR Clean-up Kit (AP-PCR-500G; Corning, NY, USA) was used to separate, extract and purify the PCR products, and the products were quantified using a Quant-iT PicoGreen dsDNA Reagent (P7581, Thermo Scientific, Waltham, MA, USA).