TAD-seq of top 2-5 percent GFP cells expressing TAD-PYL1 after 48 hours of ABA treatment, sort 1
Performed nested PCR on the purified genomic DNA using primers targeting T7 promoter and PYL1 of the backbone vector in the first step creating a ~470 bp product. Products of the first reaction were then pooled and amplified for an additional 10 steps using primers targeting outside the Gateway sites creating a ~300 bp product. Libraries were quantified on Qubit dsDNA Broad Range kit and paired-end sequenced on an Illumina MIseq with a custom PAGE-purified R1 sequencing primer.