GSM5516794: iBAT ZT_15 rep2; Mus musculus; RNA-Seq
Total RNA of snap-frozen iBAT (approx. 10-20 mg) was isolated using the nucleospin kit (Macherey-Nagel, Düren, Germany; 740955.50) after homogenization by a FastPrep-24™ 5G bead beating grinder and lysis system (4.0 m · s-1, 10 s; MP Biomedicals™, Santa Ana, California, USA) in a mixture of 400 µL RA1 (Macherey-Nagel, Düren, Germany; 740955.50) with 4 µL β-mercaptoethanol in Lysing Matrix D tubes (MP Biomedicals™, Santa Ana, California, USA; 116913500). RNA was quality-controlled on a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, California, USA). A stranded library was constructed for 100bp paired-end sequencing on BGI's platform.