Illumina HiSeq 4000 sequencing; 3' UTR RNA-sequencing of human EGFR-mutant lung adenocarcinoma cell lines treated with trametinib or controls
3' UTR RNA-sequencing of human EGFR-mutant lung adenocarcinoma cell lines treated with trametinib or controls
Cells were treated for 72h as indicated and then harvested by trypsination. RNA was extracted using Qiagen RNeasy kits. Sequencing libraries were prepared using the QuantSeq 3' mRNA-Seq library kit (Lexogen, Austria)