GSM5550764: M0 RAW264.7 cells replicate3; Mus musculus; RNA-Seq
Total RNA of RAW264.7 cells was extracted using  a Quick-RNA Microprep Kit (ZYMO RESEARCH). Polyadenylated mRNA was separated from 200 ng of total RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB). Sequencing libraries were generated using NEBNext Ultra RNA LibraryPrep kit for Illumina (New England Biolabs) according to manufacturer's instructions. Library concentrations were assessed by both a Bioanalyzer (Agilent) and NEBNext Library Quant Kit for Illumina (New England Biolabs).