sequencing of the original plasmid pool of the ORFeome-PYL1 library
Amplicons of the ORFeome were generated using Gateway-specific primers. After quantifying DNA using the Quant-iT 1X dsDNA HS kit (Thermo Fisher Scientific, Q33232), 50 ng per sample was processed using the Illumina DNA Prep, (M) Tagmentation kit (Illumina, 20018705), with 6 cycles of amplification. 2 l of each purified final library was run on an Agilent TapeStation HS D1000 ScreenTape (Agilent Technologies, 5067-5584). The libraries were quantified using the Quant-iT 1X dsDNA HS kit (Thermo Fisher Scientific, Q33232) and pooled at equimolar ratios after size-adjustment. The final pool was quantified using NEBNext Library Quant Kit for Illumina (New England Biolabs, E7630L) and  paired-end sequenced on an Illumina MIseq.