GSM6246291: WatR-FLAG strain, input; Methylomonas sp. DH-1; ChIP-Seq
Cells were lysed with lysozyme treatment, and the lysate were sonicated, immunoprecipitated with anti-DDDDK antibody. RNase I and proteinase K were treated to eliminate RNA and proteins, and DNA was reverse crosslinked by NaCl with heat treatment. Final DNA eluent were precipitated by phenol-chloroform-isoamylalcohol method. For input, the lysate was directly precipitated. 1 ng of prepared DNA was proceeded to sequencing library construction by using NEBNext Ultra II DNA Library Prep Kit for Illumina (#E7645, NEB) following manufacturer's instructions.