GSM5584021: Cd11b+Mac Mettl3-LysM-KO rep2; Mus musculus; RNA-Seq
The total RNA was extracted from CD11b+F4/80+ spleen macrophages using miRNeasy mini kit  (GIAGEN). 1 μg of total RNA was subjected to library synthesis using the TruSeq V2 RNA-Seq kit.  1 μg of total RNA was enriched for the Poly-A mRNA and reverse transcribed to double-stranded cDNA. Sequencing was performed using 12pM hybridization to a 2x100 paired end flow cell.