GSM5005704: Untreated-2 (5-FU control); Mus musculus; ATAC-seq
Sorted HSCs were lysed with ATAC-resuspension buffer containing detergents, which are listed in a previous study(Corces et al., 2017 Nat Methods 14, 959-962.). After optical amplification by PCR using ATAC-Primers (Buenrostro et al., 2015 Curr Protoc Mol Biol 109, 21 29 21-21 29 29.), these DNA fragments were purified libraries were sequenced by Next-seq.