RNA-seq of sorted GFP-high cells from non-targeting control infected DLD1 cells (NT20): Sample BP103
RNA was extracted using Trizol (Ambion 15596026) per the manufacturer's instructions then purified using the Qiagen QiaEasy RNA clean up kit (74204). RNA quality was analyzed by Bioanalyzer (Agilent RNA Pico). Poly-A pull-down was used to enrich mRNAs from total RNA samples. Libraries were constructed using Illumina TruSeq stranded mRNA kit. RTA (Illumina) was used for base calling and bcl2fastq2 (version 2.20) was used for converting BCL to fast format, coupled with adaptor trimming.