RNAseq of D. melanogaster, testes of long or short sperm
Testes from 100 males were dissected under ether anesthesia in sterile Grace's insect medium, washed in fresh droplet, stored in Trizol at -80 C until RNA extraction with Trizol-choloform protocol adapted for low sample volume. Poly-A selection library sequenced across two lines (6, 2) using paired-end design (1, 2).