GSM6208732: E14.5 UB Cells, HNF-1β deficient, biol rep 1; Mus musculus; RNA-Seq
E14.5 kidneys were harvested and made into single cell suspensions by incubating in collagenase solution followed by incubating in trypsin solution. Ureteric bud cells were isolated by fluorescence-actived cell sorting directly into Qiazol RNA lysis buffer. Total RNA was isolated using the Qiagen miRNAeasy kit. Libraries were prepared using the New England Biolabs NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina following the manufacturer's protocol. cDNA was amplified for 12 PCR cycles and fragmented using the NEBNext Ultra II FS Enzyme Mix.