A
the targeted DNA fragment was extracted and purified by the BluePippin automatic nucleic acid gel cutter (Sage Science, Beverly, MA, United States), and then perform damage repair and end repair to the DNA. After the magnetic beads are purified, using the NBD103 and NBD114 kits (Oxford Nanopore Technologies Ltd, UK) to connect the DNA ends barcode label, repurified, connected the sequencing adapter