16S sequencing of soils under tillage and residue management in Akron, CO
Soil DNA was extracted from 0.25 g of 2mm sieved soil samples.The DNA quality and quantity were determined by using a NanoDrop Spectrophotometer. Sequencing libraries were constructed by the amplification of the V4 region of the 16S rRNA gene using primers 515F and 806R. To generate the DNA libraries for subsequent sequencing, we amplified each sample in duplicate in 25 l PCR mixtures using a one-step PCR reaction with individually barcoded Illumina adapters ligated to the relevant primers. All sample libraries were pooled, cleaned, and normalized using the ThermoFisher Scientific SequalPrep normalization plate kit.