Illumina DNA sequencing of Turicibacter bilis ISU324
Briefly, a 48 h old culture had total genomic DNA extracted with the PureLink genomic DNA extraction minikit (Life Technologies, Carlsbad, CA) and resultant DNA was assessed using gel electrophoresis, a NanoDrop spectrophotometer (ThermoFisher Scientific, Waltham, MA), and a Qubit 4 Fluorometer (double-stranded DNA broad-range kit; ThermoFisher Scientific, Waltham, MA). This genomic DNA preparation was used for sequencing using Illumina sequencing technologies. A Nextera Flex barcoding kit (Illumina, San Diego, CA) was used to prepare a DNA library for sequencing on an Illumina MiSeq instrument (Illumina).