GSM5516490: MOC2K17KOEX1Bulk, 29; Mus musculus; RNA-Seq
Fresh tumors were snap frozen in liquid nitrogen, placed into tissueTUBE (Covaris) and pulverized Cryoprep Pulverizer (Covaris). Total RNA was isolated by addition of 1ml or TRizol (Thermo Fisher Scientific) using RNA-binding columns (Qiagen RNA isolation kit). On column-bound RNA was treated with RQ1 RNase-free DNase (Promega) for 30 min at room temperature, washed with washing buffers, and eluted in RNase-free water. Pooled libraries were sequenced on Illumina NovaSeq 6000. RNA-Seq analysis was done using R and Bioconductor analysis framework.