RNA-seq of Homo sapiens: Mtb infected monocytes: Sample SA_890-00425-8_rna_mono_batch1_mtb
CD14+ monocytes were isolated from cryopreserved PBMCs from tuberculosis resistant individuals (RSTR) and those with latent tuberculosis infection (LTBI). Monocytes were cultured in media alone or with Mycobacterium tuberculosis strain H37Rv (MOI 1) for 6 hours. Cells were Trizol-lysed and RNA extracted using Qiagen miRNeasy micro columns. cDNA libraries were prepared using Takara, SMARTer RNAseq Kit - Pico Input Mammalian.