GSM6208857: d28; Saccharomyces cerevisiae; ATAC-seq
5 million yeast cells were pelleted by centrigugation (2min/4600rpm) and the cell wall was digested with Zymolyase100T ATAC-seq libraries were generated as detailed in Hendrickson et al., 2018, using the Illumina Tagment DNA TDE1 Enzyme Kit for genomic DNA tagmentation. Then the libraries were amplified using the NEBNext Hi-Fidelity 2X PCR Master Mix and the primers descriven in Buenrostro et al., 2013, and the product was size-purified by Ampure XP magnetic beads.