GSM5584788: testis-sheep, scRNA-seq; Ovis aries; RNA-Seq
A 3-month-old healthy male Hu sheep was selected and castrated for testis collection, then single cell suspension was obtained using enzymatic digestion method for single cell sequencing. Single cells were suspended in calcium- and magnesium-free PBS containing 0.25% trypsin/ethylenediaminetetraacetic acid (EDTA),Cells  were resuspended in DMEM/F-12 . RNA libraries were prepared with Chromium Single cell 3 Reagent v3 Kits according to the manufactures protocol.