GSM6333474: ATAC-seq_Thp1-mono-rep2; Homo sapiens; ATAC-seq
100,000 cells were centrifugation at 800 g for 5 min and then washed once using 500 µl of cold 1× PBS and centrifugation at 800 g for another 5 min. Cells were lysed using cold lysis buffer (10 mM Tris-HCl (pH 8.0 at 25 °C), 10 mM NaCl, 0.3% Igepal CA-630). After lysis, nuclei were spun at 800g in 4 °C for 10 min. The pellet was resuspended in the transposase reaction mix (10 µl 5× TTBL buffer (Vazyme, TD501-02), 5 µl TTE Mix V50 buffer (Vazyme, TD501-02) and 35 µl ddH2O water). Tagmentation was carried out for 10 min at 55 °C. Immediately following transposition, The DNA was purified using a Qiagen MinElute PCR Purification Kit (Qiagen, 28004) and eluted with 10 μl elution buffer. The ATAC-Seq library was amplified by the primers in TruePrepTM Index Kit V2 for Illumina® (Vazyme, TD202).