Bisulfite-Seq of Sus scrofa: oocyte whole genome bisulfite sequencing
Total DNA was extracted from Sus scrofa oocytes by QIAGEN QIAamp DNA Micro Kit. BS-seq library was constructed from the oocyte total DNAs by Zymo Pico Methyl-Seq Library Prep Kit, according to the manufacturer's instructions.  Each NGS library was sequenced by Illumina NovaSeq instrument using 100 bp paired-end sequencing chemistry.