GSM5309263: silica crystals; Mus musculus; RNA-Seq
https://doi.org/10.1038/nprot.2009.95 - http://dx.doi.org/10.1038/ni.1631 - https://doi.org/10.1172/jci.insight.126556 The cells were partitioned into nanolitre scale Gel Beads in emulsions (GEMs) and lysed using the 10x Genomics Single Cell 3′ Chip V3.1 GEM, Library and Gel Bead Kit (cat: PN-1000121). cDNA synthesis and library construction were performed as per the manufacturer's instructions. The RNA was reversed transcribed and amplified using 14 cycles of PCR. Libraries were prepared from 10μl of the cDNA and 12 cycles of amplification. Each library was prepared using Single Index Kit T Set A (cat: PN-1000213). Libraries were pooled according to estimated cell load, the pool was sequenced at 200pM on the HiSeq4000 system (Illumina) using the configuration 28-8-98 on a single-index paired-end run.