GSM5960850: Test 1; Homo sapiens; RNA-Seq
Cell lysates were collected and total RNA was extracted using Trizol reagent.About 1 ug of total RNA was used to generate NGS sequenicng libraries. NGS sequencing libraries was prepared using TruSeq RNA Sample Prep kit (Illumina). The Poly-a containing RNA molecules were prurified and fragmented which are reverse transcribed into cDNA with random primers. The short fragments were purified with QiaQuick PCR extraction kit and connected with sequencing adapters and each library was separted by adjoining distinct MID tags. The resulting cDNA libraries were then paired-end sequenced (2 x 101 bp) with NovaSeq 6000 system (Illumina).