GSM5007795: TCT_NI_V6; Mus musculus; RNA-Seq
Distal colons and ileum tissues were snap-frozen and powderized in liquid nitrogen with a tissue homogenizer. RNA was extracted using the RNeasy Plus Universal kit from Qiagen, following the manufacturer's instructions. RNA concentration and quality were evaluated in Agilent Bioanalyzer/TapeStation. 1µg of RNA was used for RNA-Seq library preparation. Poly-A enrichment was performed to increase mRNA amount in the input RNA. RNA-Seq library was prepared using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® following the manufacturer's instructions.