GSM5464073: xpf-6; Caenorhabditis elegans; RNA-Seq
To extract total RNA, worm or tissue pellets in Trizol underwent six freeze-thaw cycles in a dry ice-ethanol bath. RNA was extracted according to the Trizol procedure, and resuspended in RNase- and DNase-free water. RNA from whole worms was quantified using the Qubit 2.0 fluorometer (Invitrogen) A total amount of 1 μg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext®Ultra™RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample