GSM5465639: lg2-SAM-rep1; Zea mays; RNA-Seq
Poly-A+ RNA-seq libraries were generated from pools of hand-dissected inflorescence primordia. ligule and SAM from wild-type (B73) and maize mutants. RNA was extracted (PicoPure RNA isolation kit; Thermo Fisher Scientific) with the following adjustments. 40 µl and 60 µl of RNA extraction buffer were added respectively to stage 1 and stage 2 ground tassels. After 30' incubation at 42°C, samples were centrifuged at 800g for 2'. Equal volume of 70% EtOH was added to the sample and processed normally according to the kits directions. library preparation was outsourced to Novogene (USA) RNA-seq libraries were processed using an Illumina HiSeq 4000 platform at Novogene with a 150bp paired-end sequencing design.