GSM5857055: peripheral blood mononuclear cells_CLP; Mus musculus; RNA-Seq
The bood was incubated in dissociation buffer containing 1 mg/ml hyaluronidase（sigma,H3506） and 20,000U/ml DNase I（sigma,DN25） for 15 min at 37 C. Enzymatic digestion was quenched with Dulbecco's Modifified Eagle Medium (DMEM, Gibco) supplemented with 10% fetal calf serum (FCS, 10270106, Gibco). Cells were resuspended at a concentration of 1 million cells per ml and stained with PBST containing 1% FCS The Chromium Single Cell 3′Library & Gel Bead Kit v3 (10X Genomics, 1000075) was used for single-cell barcoding, cDNA synthesis and library preparation，following manufacturer's instructions as previously described. Libraries were sequenced on Illumina novaseq 6000 using paired-end 150 bp.