GSM5857221: MRT_hotair_nanopore_65s; synthetic construct; RNA-Seq
cDNA was prepared using gene-specific RT primers. For nanopore sequencing, the cDNA was first converted to double-stranded DNA, and then Oxford Nanopore Ligation Sequencing Kit and Native Barcoding were used to construct the libraries. For Illumina TruSeq, Illumina TruSeq forward primer and indexed reverse primers were used to build the libraries.