GSM5464263: GT_WT_rep1; Homo sapiens; RNA-Seq
50,000 cells were lysed in ATAC lysis buffer and tagmented (Illumina Nextera DNA Library Preparation Kit). After adding SDS, the samples were purified with 2X Agencourt AMPure XP beads (Beckman Coulter A63881) Samples were amplified using NEBNext Q5 Hot Start HiFi PCR Master Mix. The concentration of samples was checked using PicoGreen and median fragment size was checked using the Agilent D1000 screentape on the Agilent Technologies 2200 TapeStation. Samples were sequenced PE50 on a HiSeq 2500.