GSM6246436: y w, Pngl-ex14, Pngl-ex14 replicate 2; Drosophila melanogaster; ssRNA-seq
For each genotype, 40 midguts were dissected directly in the TRI Reagent (Millipore/Sigma, T9424) and stored at -80°C. The samples were prepared on a Beckman Fxp using the Illumina TruSeq stranded mRNA chemistry and sequenced on a NextSeq 500 (Mid output flowcell) in paired-end mode. 