GSM5396639: FoB_BR1; Mus musculus; RNA-Seq
Total RNA was extracted using RNeasy Mini Kit (Qiagen) cDNA was generated from polyadenylated transcripts using SMART-Seq® v4 Ultra® Low Input RNA Kit (cat # 634891, Takara Bio). mRNAseq libraries were prepared with Nextera XT DNA Library Preparation Kit (cat# FC-131–1096, Illumina) and quantified using KAPA library quantification kit (Roche). mRNA-seq libraries were run on a HiSeq 2500 RapidRun generating 50bp single-end reads.