GSM5599217: E11_E14; Mus musculus; RNA-Seq
RNA was isolated within the droplets according to the inDrops protocol (Klein et al. Cell 2015, Zilionis et al. Nature Protocols 2017). polyA RNA captured via inDrops platform 2 libraries of approximately 3000 cells were collected from each biological replicate: pools from animals from the same litter for embryonic and early postnatal stages (E11 - 3 litters, 35 embryos total; E14 - 3 litters, 33 embryos total; E17 - 2 litters, 8 embryos total; P3 - 2 litters, 4 pups total), or individual animals for later postnatal stages (P10 - one male and one female pup from the same litter; P30 - two male and two female animals from the same litter). inDrops library preparation was performed as previously described (Klein et al. Cell 2015, Zilionis et al. Nature Protocols 2017) Libraries were sequenced on the Illumina NextSeq system. custom inDrops single-cell RNA-Seq