GSM5464747: CM_01: Covid 19 infected treated with IgG-rep 1; Mus musculus; RNA-Seq
Snip from lower inferior lobe was taken and RNA was extracted from murine lung tissue preserved in trizol by bead beating (tissuelyserII, Qiagen), followed by a phenol-chloroform extraction and RNA Isolation Kit (Qiagen). RNA quality was assessed using Agilent Tape station. Ribosomal RNA was depleted using the rRNA depletion kit (NEB E6310). Following rRNA  2 depletion cDNA libraries were prepared using the NEB ultra-directional library preparation kit  3 2.0 (NEB E7760) and indexed using the NEBNext Multiplex Oligos for Illumina (NEB E7335, E7500). Libraries were diluted and pooled. 15 libraries were sequenced on the Illumina Nextseq 500 using a 150 high output kit (400 Million 8 reads, 2x75bp paired end, 150 cycles)