ITS amplicons of grass litter
We extracted DNA from standing dead Stipagostis sabulicola (Poaceae) stems using a MoBio PowerSoil DNA extraction kit supplemented with 10 minutes of sonication prior to mechanical lysis.  We sequenced the ITS region (ITS1-F: CTTGGTCATTTAGAGGAAGTAA; ITS2: GCTGCGTTCTTCATCGATGC) using 250-bp paired-end sequencing on the MiSeq Illumina (V2) platform at Michigan State Universitys Research Technology Support Facility Genomics Core.