16S rRNA gene amplicon sequencing of stool sample [JMF-1902-6-0150A]
Stool microbiome composition was determined using amplicon sequencing targeting the V4 region of the 16S rRNA gene using widely applied primer pairs which target both bacteria and archaea. Stool samples were collected within the same week of blood collections for each subject and stored at -20 degree C until processing. Total DNA was extracted from all samples on the QiaCube (automated extraction) using the Qiagen QIAamp Fast DNA Stool Minikit.