Tomato
RNA quality and concentration were measured by agarose gel electrophoresis through NanoPhotometer spectrophotometer (Implen, Germany) and Agilent 2100 BioAnalyzer (Agilent Technologies, USA), respectively. The cDNA of about 250  300 bp was screened with AMPure XP beads. PCR amplification products were repurified with AMPure XP beads to obtain the library. A Qubit 2.0 fluorometer (Invitrogen, USA) was used for preliminary quantification. The library was diluted to 1.5 ng/l, and the insert size of the library was detected via the Agilent 2100 BioAnalyzer. Then, the Novaseq 6000 platform sequencing was performed.