16S of Parmigiano Reggiano natural whey starter
The V3-V4 region of bacterial 16S rRNA gene was amplified using the primers 341F and 805R. A second PCR was then carried out to attach dual Illumina barcode indices and adapters using the Nextera XT library preparation kit (Illumina, San Diego, CA). PCRs products were purified and normalized using a SequalPrep Normalization Plate. The 16S rRNA gene library was sequenced at BMR Genomics (Padua, Italy), using the Illumina MiSeq V3 platform (Illumina, San Diego, CA, USA).