WGS of Bacidia gigantensis
DNA was extracted from whole thallus material using a silica columns and a protocol similar to commercially available kits5. A bead clean up at a 0.6:1 bead to sample ratio was first performed to remove small DNA fragments using PCR Clean DX C-1003-450 (Aline Biosciences, Woburn, MA, USA).  Quality of the gDNA was assessed using pulse-field gel electrophoresis (PFGE) using 1% agarose gel by running with 0.5X TBE on a CHEF pulse field gel apparatus (BioRad, Hercules, CA, USA) for 22 hours. Library construction and sequencing were done by following the Oxford Nanopores Genomic DNA by Ligation (SQK-LSK109) protocol.