GSM5419495: 43L; Mus musculus; RNA-Seq
1 day after Ischemic-reperfusion-injury, the renal tubules from control mice, mild injury (20min-ischemia) or severe injury (40-min ischemia) (n=3 in each group) were isolated. Total RNA were extracted from these isolated tubules by TRIzol reagent. For the preparation of RNA-seq library, total RNA (3μg) from each sample were treated with the VAHTS Total RNA-seq (H/M/R) Library Prep Kit for Illumina (Vazyme Biotech, Nanjing, China) to erase ribosomal RNA but retained mRNA and non-coding RNA (ncRNA). Purified RNA was digested with RNase R (Epicenter) followed by purification with TRIzol (Invitrogen). RNA-seq libraries were constructed using the KAPA Stranded RNA-Seq Library Prep Kit (Roche) and subjected to deep sequencing with an Illumina HiSeq 4000 (Aksomics).