GSM5465175: DG-15_S73; Mus musculus; RNA-Seq
To perform single-cell RNA-seq analyses, GV-stage oocytes (18 oocytes/genotype) were individually placed in Eppendorf tube containing 10µL of nuclease-free water. Tubes were snap frozen in liquid nitrogen and stored at -80 o C until further experiment. The whole lysate was supplied for cDNA synthesis and RNA-seq library preparation with, respectively, a SMARTer Ultra Low Input RNA Kit (Clontech, CA USA) and Nextera XT library prep kit (Illumina, CA, USA), each according to the manufacturer's instructions. Indexed libraries were pooled (10 nM each) and sequenced using an Illumina HiSeq 2500 System under single-end, 100-bp conditions. single-cell RNAseq (SMART-seq)