NextSeq 500 sequencing; The embryonic node functions as an instructive stem cell niche (bulk RNA-seq)
The embryonic node functions as an instructive stem cell niche (bulk RNA-seq)
Tissues (HH8 node sub-regions) were isolated from transgenic-GFP embryos using 30G syringe needles in sterile molecular grade PBS. Tissues were collected into RNAlater (Invitrogen, # AM7020). For each sample, tissues were collected from thirteen to seventeen embryos. RNA was extracted using the Micro Total RNA Isolation Kit (Invitrogen, # AM1931) and concentration and quality measured using Tapestation The NEBNextÂ Single Cell/Low Input RNA Library Prep Kit for Illumina (# E6420) was used for cDNA and library synthesis