GSM5625357: SymE_untagged_30min_Rep2 [ChIP-seq]; Escherichia coli; ChIP-Seq
For step by step protocol see publication. Briefly: Samples were taken and crosslinked with formaldehyde. Cells were then lysed and sonicated, incubated with anti-FLAG antibodies and then immune complexes were pulled out with Protein A dynabeads. Beads were washed and then eluted. Crosslinking was reversed and DNA was extracted using a phenol-chloroform extraction. DNA samples to be sequenced were end-repaired, 3' adenylated and sequencing adaptors were ligated on. Libraries were then PCR amplified and submitted for sequencing