DNA-Seq of Oropouche Virus
DNA samples were fragmented by Covaris M220 Focused-Ultrasonicator (Covaris Ltd, Brighton, UK) using microTUBE-15 to 350 bp. The TruSeq Nano libraries prep kit (Illumina) was used following the instructions of the kit manufacturer except 15 cycles of amplification due to the low amount of starting materials. Sequencing was carried out on Illumina MiniSeq platform at a depth of eight million reads total.