Drosophila_PacBio_HiFi_UltraLow
gDNA was sheared with g-TUBE (Covaris). 10 ng of sheared DNA was processed according to Preparing SMRTbell Libraries from Ultra-Low DNA Input from PacBio using SMRTbell gDNA Sample Amplification Kit and SMRTbell Express Template Prep Kit 2.0. Library was size selected with BluePippin (Sage Science) to an average insert length of 11 kb. In SMRTLink v9.0, HiFi reads were generated with Circular Consensus Sequencing, PCR primer adapter sequences were trimmed with Trim PCR Adapters and PCR duplicated reads were removed with Mark PCR Duplicates. Reads with residual PCR primer adapter sequences were removed with filterprimer (https://github.com/armintoepfer/pacbio-toolbox/blob/develop/filterprimer.cpp).