Tuberous roots of first generation (planted only one time in field, biological replicate 3, FGP3)
Roots of FGP3 P. heterophylla were obtained from critical stages of P. heterophylla plants that viral symptoms obviously appeared in.Total RNA satisfied quality for building of sRNA library were separated by PAGE gel electrophoresis to screen the ranges of RNA from 18 to 30 nt, followed by sRNA located in this regions were sequentially ligated with 3 and 5' adaptors. Resulting products further separated by 10% denatured PAGE and reversely transcripted to construct cDNA library of small RNA, which were then finally sequenced in the BGISEQ-500 platform.