GSM5419423: HC1887; Mus musculus; OTHER
Lung tissue was extracted temporarily frozen for later processing. Prior to tissue homogenization and proteinase K digestion, each lung sample was spiked with three cell lines bearing known barcodes (5.00E+05 cells of each cell line). Genomic DNA was then isolated by phenol-chloroform extraction followed by ethanol precipitation from the total lung tissue of each mouse. One-step PCR targeting lentiviral barcodes to append Illumina adapters, unique dual indices for multiplexing, and six to nine random bases to increase sequence complexity. Custom Amplicon Library (Tuba-seq)