GSM5362620: KO3CpG2h; Mus musculus; RNA-Seq
purification protocol: For RNA-Seq, BM-derived pDCs (CD3-CD19-CD11c+CD11blowB220+SiglecH+mPDCA1+) from wild-type and Batf-deficient mice were FACS purified using FACS Aria III (BD). Libraries were prepared according to Illumina® 'TruSeq Stranded mRNA Library Prep Kit'. Briefly, 200 ng total RNA were used for mRNA capturing, fragmentation, the synthesis of cDNA, adapter ligation and library amplification. Bead purified libraries were normalized and finally sequenced on the HiSeq 3000/4000 system (Illumina Inc. San Diego, CA, USA), following the manufacturer's protocols, with a read setup of 1 × 150 bp.