R-gene SeqCap
Leaf material were collected from three-four plants and frozen in liquid nitrogen  at -180oC. Total RNA was extracted with RNeasy Plant Mini kit (Qiagen) including DNase treatment. CDNA library construction,sequence capturing and sequencing were done at Liverpool Genomics Center,UK. Sequencing was performed on Illumina Hiseq 2500 instrument by paired-end sequencing as per manufacturers instructions.