GSM5464042: Ex1-RIF100-B; Mycobacterium tuberculosis; RNA-Seq
After incubation in RNAprotect, samples were lysed in TRIzol (Invitrogen) with bead beating, and RNA was extracted using RNeasy mini kit (Qiagen) as per manufacturer protocol. Sample libraries were prepared for RNA-seq using the Ribo-Zero Magnetic Bacterial kit (Epicentre) in conjunction with TruSeq Stranded Total RNA kit (Illumina).