GSM5897928: INV410; Plasmodium falciparum; RNA-Seq
Parasite schizont-enriched material from individual isolates was stored in either TRIzol® or RNAlater™ (Thermo Fisher Scientific, MA, USA), and RNA was extracted by phenol-chloroform and cleaned up using the NucleoSpin® RNA XS extraction kit (Macherey-Nagel, Germany). RNA was reverse transcribed and cDNA amplified using the SmartSeq® v4 Ultra® Low Input RNA Kit for Sequencing (Takara Bio. Inc., Shiga Prefecture, Japan). Successfully amplified samples were prepared for paired end short-read sequencing using the Nextera XT Library Prep kit (Illumina, California, USA), according to manufacturer instructions