GSM5651454: 1_WTA; Mus musculus; RNA-Seq
TG tissue was extracted and placed in the pre-cooling papain solution. Then, slightly minced or cut into small pieces, tissue was incubated in the solution at 37 ℃ with constant agitation for 30 to 90 min. The digestive system was centrifuged at 300 g for 5 min at room temperature and the cell pellet was resuspended in DNase dilute albumin-inhibitor solution. Then, the cells isolated by density gradient centrifugation were resuspended in complete medium to obtain single-cell suspensions. Libraries were sequenced utilizing the Illumina NovaSeq platform