GSM5914497: D 0 high concentrate feeding replicate 4; Bos taurus; miRNA-Seq
Extraction of total RNA was extracted with Trizol according to manufactures specifications. Qantificatioon and qualification were checked by  NanoPhotometer spectrophotometer and Agilent Bioanalyzer 2100 system. cDNA libraries were constructed using Ion Total RNA-Seq Kit v2 according to the manufacturer.