GSM5465281: F5 - mediastinal adipose tissue  pool 3 - repl. 2; Mus musculus; RNA-Seq
Thymi, mediastinal adipose tissuse and aortic arches of five female, 8 week old C57BL/6J mice were collected and pooled. Aortic arches and mediastinal adipose tissue were digested as previously described (PMID: 14597735) and single cell suspensions were generated from all three tissues. 400 viable CD4+CD8b+TCR- cells were sorted into 8ul low-input lysis buffer containing RNaseH inhibitor, dNTPs, and 0.1% Triton X-100 in replicates Libraries of 200 cells (4 ul) were prepared according to Illuminas's instruction with previously published modifications (PMID: 29956159). In brief, cDNA was amplified for 18 cycles to generate sufficient material for sequencing. Librariers were sequenced at 50 bp single end reads