GSM5419514: e1ne; Homo sapiens; RNA-Seq
Skin samples were obtained from 11 EBS patients with 4mm punch biopsies. 9 biopsies were taken from a site of an acute blister (occurrence within 1 week of biopsy), and 11 biopsies were taken from a site without any blistering. All samples were taken from the flank or upper arm for ease of wound care. In blisters, the dermis and epidermis self-separated without need for additional manipulation. In non-blistered skin, the dermis and epidermis were processed via one freeze-thaw cycle of liquid nitrogen. RNA preparation, library construction, and sequencing were performed by the Beijing Genomics Institute.