Illumina HiSeq 2000 paired end sequencing
The DNA was sheared into 100-1500bp fragment size with the peak around 300-500bp using the�Adaptive Focused Acoustics system (Covaris). The libraries were constructed using NEBNext DNA�Library Prep Master Mix Kit (New England Biolabs) following the manufacturer�s recommendation.�The libraries were sequenced on�Illumina Hiseq 2500/2000 with paired-end read of 101bp
libraries were constructed using NEBNext DNA�Library Prep Master Mix Kit (New England Biolabs) following the manufacturer�s recommendation.