GSM6336805: HFF-tert, doxycycline-inducible ICP22, 5ug/ml doxycycline for 24h, no KCl, rep2; Homo sapiens; ATAC-seq
Infected cells were harvested by scraping at the indicated time points, washed, lysed with mild lysis buffer according to the published OMNI-ATAC-seq protocol, incubated with Tn5 transposase (tagmentase) for 30 minutes, lysed in Qiagen's PB buffer, DNA purified according to the MinElute protocol. Libraries were prepared using Illumina-compatible primers (like Nextera primers but custom sets with dual-unique barcodes). Initially, quantitative PCR was performed to judge how many cycles are required for optimal library preparation. 36bp paired-end, NextSeq 500 (Illumina)