GSM5898931: Prdm16-KO+shAppbp2+cAMP inguinal white adipoctyes mRNA rep. 2; Mus musculus; RNA-Seq
Total RNA was isolated using Zymo direct-zol RNA prep. Extracted RNA was treated with NEBNext rRNA Depletion Kit v2. RNA was converted into double stranded cDNA using NEBNext iWAT mRNA Second Strand Synthesis Module. cDNA was tagmented and amplified for 12 cycles by using Nextera XT DNA Library Preparation Kit.