Illumina sequencing of Enterococcus faecium 1,231,410 pbpA mutant
Genomic DNA were purified and subjected to Nextera DNA Flex library preparation according to the manufacturers instructions (Illumina). For normalization, the libraries were quantified by Qubit (Invitrogen) and the average size of the libraries was determined using the High Sensitivity NGS fragment analysis kit on the Fragment Analyzer (Agilent). The normalized libraries were pooled and sequenced on an Illumina NextSeq500 sequencing platform with 76-bp paired-end reads.