BLS_OTC3_bac.comm
One-step PCR was performed using dual-indexed fusion primers, including Nextera P5/P7 adapters, i5/i7 indices, and target-specific forward/reverse primers. Integrated Microbiome Resource (IMR) wet-lab protocols can be found at Microbiome Helper GitHub (https://github.com/LangilleLab/microbiome_helper/wiki/Microbiome-Amplicon-Sequencing-Workflow).