GSM5699439: DP_thym_KO1; Mus musculus; RNA-Seq
Single cell suspesion was purified from the thymus and sorted for CD4+/CD8+ DP thymocytes were sorted by FACS BD Aria with a purity more than 98%. RNA was extracted with RNAeasy® Mini Kit according to the protocol of the manufacture (Qiagen). Libraries were generated using the Illumina TruSeq preparation kit