GSM5552449: Cortical neuron-CDH12 siRNA; Rattus norvegicus; RNA-Seq
Total RNA was collected and purified on the Illumina TruSeq Stranded mRNA LT sample preparation kit (Illumina Inc.) per manufacturer's instructions. The RNA integrity and quantity was mea_x0002_sured by Nanodrop (Thermo Scientific) For this mRNA enrichment, the rRNA removal kit was used to interrupt all se_x0002_quences into 200–300 bp. Complementary DNA was synthesized from enriched and fragmented RNA using Super Script II Reverse Transcriptase (Invitrogen Corp.) and random primers. After the library was constructed using the dsDNA converted from cDNA, RNA-Seq libraries were qualified by the Agilent 2100 Bioanalyzer (Agilent Technologies).