GSM5354584: MDA-MB-231 down2; Homo sapiens; RNA-Seq
Libraries were prepared from 600 ng total RNA using the TruSeq Stranded mRNA kit (Illumina) following the manufacturer's instructions. The different steps are the PolyA mRNA capture with oligo dT beads, cDNA double strand synthesis, adaptors ligation, library amplification and sequencing. RNA sequencing was performed using the NextSeq500 Illumina sequencer in 75 bp paired-end.