GSM5914443: Strand-specific RNA-seq in VINK-2 siRNA treated cells- Replicate #2; Homo sapiens; RNA-Seq
Total RNAs were prepared using the MasterPure RNA Purification Kit (Epicentre Biotechnologies) supplemented with Baseline-ZERO DNAse (Epicentre). EMBL-GeneCore performed the ribosomal RNA depletions. Library construction (NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®) and sequencing were performed by EMBL GeneCore (Heidelberg, Germany) (stranded rRNA-minus RNA-seq). Samples were barcoded, sequenced paired-end  in one run of Illumina NextSeq 75PE high for 8 samples, and demultiplexed.