GSM6248589: EndoC-βH1 HNF1A sc-6547 - input; Homo sapiens; ChIP-Seq
Cells were dissociated using TrypLE, cross-linked, and extracted for nuclear lysates. The lysates were sonicated (from which input DNA was obtained as control) and incubated with the respective ChIP antibodies. Immunoprecipitation was performed and the genomic DNA was extracted and purified for sequencing analysis. Library preparation was done using a commercially available kit, NEB# E7645 NEBNext Ultra II DNA Library Prep Kit for Illumina KIT® following the manufacturer's protocol. During the library construction, the ChIP-ed DNA underwent End Repair, Adapter ligation, size selection (for ~150bp insert)  and 16 cycles PCR enrichment to generate the final sequencing ready library. The quality of the library was checked using Agilent D1000 ScreenTape. The final library should have a fragment size of ~270bp insert plus adaptor and PCR primer sequences). The quality of the library was checked using Agilent D1000 ScreenTape. A single peak was expected and was observed indicating that the library was good and suitable for sequencing.