GSM5552741: 9086407 Fast baseline; Homo sapiens; miRNA-Seq
RNA is extracted from 200 µL of human plasma using the QIAcube following Qiagen's instructions for sample setup of the miRNeasy Serum/Plasma Advanced Kit (Cat #217204). miRNA libraries are prepared using QIAseq miRNA Library Kit optimized for biofluid samples (QIAseq miRNA, Cat # 331505). Individual libraries are quantified using a fluorometric high-sensitivity dsDNA assay (Denovix). Library quality is assessed on a high sensitivity DNA chip on the Agilent 2100 Bioanalyzer system (Agilent). Libraries are multiplexed and sequenced on Illumina NextSeq550 to generate 75bp SE UMI-tagged reads.