GSM5396706: WT_MPP_1; Mus musculus; ATAC-seq
Nuclei were isolate from 20,000 LT-HSCs sorted from three individual Phf6 knockout (Phf6−/Y Rosa26+/Cre-ERT2) and Phf6 wild-type (Phf6f/Y Rosa26+/+) mice 8 weeks after tamoxifen treatment. Nuclei were tagmented using Nextera Tn5 Transposase with single indexed, library-specific sequencing adapters. ATAC-seq libraries were quantified based on the average fragment size according to Bioanalyzer results, pooled at equimolar concentrations, and sequenced as a pool to a total read-depth target of approximately 7 × 107, paired-end, 75-nucleotide-long reads per sample on an Illumina NextSeq 500.