GSM5100999: SC170; Homo sapiens; RNA-Seq
Explanted lungs were finely diced and digested for 1 hour in a mix of collagenase A/DNAse I.  A single cell suspension was generated by filtering lungs through 70 micron filters and resuspending cells in PBS containing 0.04% BSA.  Cells were loaded into the 10X Genomics Chromium instrument per manufacturer's directions. Libraries were constructed according to 10X Genomics' Single Cell 3' Reagent Kits v2 User Guide