DNA-Seq of plant Acmella uliginosa
Genomic DNA was extracted using a modified CTAB method.Genomic DNA for each sample was quantified with High-sensitivity assays (Invitrogen, USA) using a Qubit 4.0 Fluorometer (Invitrogen, USA) and the quality of the DNA is accessed on 4200 TapeStation System (Agilent, USA). 10500 ng of the genomic DNA used for library preparation using Illumina DNA Prep kit (Illumina, San Diego, USA) according to the manufacturers instructions. Quantification and size estimation of the libraries was performed on both the Qubit 3.0 Fluorometer and 4200 TapeStation System (Agilent, USA). Next, the library was normalized to 2 nM and sequenced on a NovaSeq Sequencer (2  150 bp paired-end reads) (Illumina, San Diego, CA)