H2_13_16s
16S rRNA gene amplification and sequencing was carried out at the Gene Expression Unit from Instituto Gulbenkian de Ciencia, following the service protocol. For each sample, the V4 region of the 16S rRNA gene was amplified using the primer pair F515/R806. Samples were then pair-end sequenced on an Illumina MiSeq Benchtop Sequencer, following Illumina recommendations.