GSM4826751: Mag2_IP_rep2; Saccharomyces cerevisiae; OTHER
Mag2-bound ribosomes were obtained by purification of Mag2. The purified fraction was treated with RNase, and RNA was extracted using TRIzol reagent (Thermo Fisher Scientific) following manufacturer's instructions. Libraries were prepared for sequencing using a customized protocol from Ingolia et al., 2012. In brief, ribosome-protected mRNA fragments were purified and ligated to a single-stranded linker that serves as a priming site for reverse transcription. The first-strand reverse transcription products are circularized and PCR-amplified to generate a deep-sequencing library.