GSM5463927: C-Ctrl-W3 miRNA-seq; Mus musculus; miRNA-Seq
The regenerative corneal epithelium was disrupted in TRIzol reagent (Invitrogen, USA) immediately and stored at -80°C until further experiments could be performed. The RNA obtained was run on agarose gels to measure degradation and contamination. RNA quantity and quality were also estimated using the NanoDrop ND-1000 system. Complementary DNA (cDNA) libraries were prepared using NEB Multiplex Small RNA Library Prep Set (Illumina, USA) for miRNA. Then, the quality of the amplified libraries was verified with a 2100 Bioanalyzer (Agilent, USA). Sequencing of high-quality miRNA was carried out using the Illumina NextSeq 500 (Illumina, USA).