GSM5699711: 12 hours stimulation - CBLB WT + anti-CD3 stimulation - Replicate 3; Mus musculus; RNA-Seq
RNA was extracted from stimulated T cell (>99% CD8+ purity) using RNeasy Mini Kit (CAT# 74104, Qiagen) according to established instructions. After quantity and quality assessment using Agilent Bioanalyzer RNA Nano 6000 Kit (CAT# 5067-1511, Agilent) and ﻿Qubit BRRNA kit (CAT# Q10210, Thermo Fisher Scientific), RNA libraries were prepared by removing ribosomal RNA using the TruSeq Stranded Total RNA kit (CAT# 20020596, Illumina). After cDNA preparation, the paired-end libraries were sequenced on NextSeq 500 (Illumina) using 100 bp protocol.