GSM4730415: TRAPSeq_5; Mus musculus; RNA-Seq
RNA was extracted from the input and TRAP samples using the RNA Clean & Concentrator Kit (Zymo Research). RNA quality and concentration were measured using a NanoDrop spectrophotometer and a High Sensitivity RNA ScreenTape (Agilent Technologies). All samples had RNA integrity scores >7.0. Barcoded libraries were prepared with the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770) and the NEBNext rRNA Depletion Kit (NEB #E6310), according to the manufacturers' instructions. Each library was screened for quality and adapter dimers on a High Sensitivity D1000 ScreenTape (Agilent Technologies), then sequenced on an Illumina HiSeq 3000 using 150bp, single-end reads.