GSM5050685: Ascending aorta patient Ao4A; Homo sapiens; RNA-Seq
Ascending and descending human aortas were obtained from 5 human patients collected in compliance with all relevant ethical regulations for human research participants with patient consent following a rapid autopsy protocol (DFHCC IRB #13-416) within 4 hours of cardiac death. Aortic segments from the aortic root, ascending aorta, descending thoracic aorta and infrarenal abdominal aorta were surgically collected by a single operator and immediately frozen and stored at -80°C until use. For nuclei isolation, aortas were mounted frozen on OCT and sectioned. Cut tissues were incubated in ice cold nuclei isolation buffer and then liberated by dounce homogenization in isolation buffer. Homogenates were centrifuged and filtered to isolate nuclei. Nuclei, stained with Trypan blue, were manually counted using a hemocytometer (inCyto.com). 7,000 nuclei (5,000 recovery) per aorta were used for droplet generation and library construction according to manufacturer's protocol (10x Genomics, V3). 10x genomics 3' capture v3