Illumina HiSeq 2000 paired end sequencing
The DNA was sheared into 100-1500bp fragment size with the peak around 300-500bp using the Adaptive Focused Acoustics system (Covaris). The libraries were constructed using NEBNext DNA Library Prep Master Mix Kit (New England Biolabs) following the manufacturer's recommendation. The libraries were sequenced on Illumina Hiseq 2500/2000 with paired-end read of 101bp
libraries were constructed using NEBNext DNA Library Prep Master Mix Kit (New England Biolabs) following the manufacturer's recommendation.