NextSeq 500 sequencing; Small RNA-Seq of plasma-derived exosomes from Acute Respiratory Distress Syndrome patients and healthy subjects
Small RNA-Seq of plasma-derived exosomes from Acute Respiratory Distress Syndrome patients and healthy subjects
Plasma-derived exosomes were isolated using standard ultracentrifugation protocol. Total RNA was extracted with the miRNeasy kit (Qiagen) following the manufacturer's protocol. Small RNA libraries (miRNA-Seq) were prepared using Clontech SMARTer smRNA-Seq Kit (Illumina) following the manufacturer's protocol.