GSM5651264: mutant lpcC replicate 4; Mus musculus; RNA-Seq
Total RNA was isolated from the spleens of mice vaccinated ID with LVS or one of the SCHU S4 deletion mutants ΔclpB, ΔgplX and ΔlpcC, (4 spleens from each group treated individually throughout) using Tri reagent (Life Technologies). Genomic DNA contamination was removed by Turbo DNA-Free Kit (Life Technologies). RNA was processed for paired-end RNA sequencing using Illumina Hi-Seq2000 (Genome Quebec). RNA-Seq Libraries were generated using the TruSeq strand RNA kit (Illumina). The RNA-Seq libraries were quantified by Qbit and qPCR according to the Illumina Sequencing Library qPCR Quantification Guide and the quality of the libraries was evaluated on Agilent Bioanalyzer 2100 using the Agilent DNA-100 chip.