GSM5419302: Naïve KO CD8+T cells rep1; Mus musculus; RNA-Seq
Naïve CD8+T cells were isolated by FACS, followed by activation with anti-CD3 and CD28 antibody for 18hr in vitro. RNA was extracted using Trizol reagent. 1 ug of total RNA was used for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols