GSM5354406: In vitro diff. hepatocytes_rep1; Mus musculus; RNA-Seq
Total RNA was isolated using RNeasy Mini Kit (Qiagen, Cat. No. 74104 50) including the DNase I treatment step (Qiagen, cat. No. 79254). 1 µg mRNA was isolated from the total RNA by poly-dT enrichment using the NEBNext Poly(A) mRNA Magnetic Isolation Module according to the manufacturer's instructions. RNA-Seq library was prepared using NEBNext® Ultra™ RNA Library Prep Kit for Illumina®. 75bp single read sequencing on Illumina HiSeq 2500.