sRNA-Seq of Chinese alligator, male testis, warmroom, winter
sRNA libraries were prepared from 3 mg RNA per sample, using a NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB) according to the manufacturers instructions. The index-coded samples were clustered using a TruSeq SR Cluster Kit v3-cBot-HS on a cBot Cluster Generation System. After cluster generation, the sRNA libraries were sequenced on an Illumina HiSeq 2500 platform, generating 50-bp single-end reads.