GSM5354545: Classical monocyte_4; Homo sapiens; RNA-Seq
RNA isolated from sorted cell type of each patient using Qiagen Rneasy micro kit  with on-column DNase digestion. cDNA synthesized from 500 pg of total RNA using Clontech SMART-seq v4 ultra low input RNA kit as per the manufacturer's protocol. Amplified cDNAs were appended with dual indexed barcodes using Nextera XT DNA library preperation kit (Illumina).