Amplicon sequencing of Carrizo B-LCY2
The -LCY2 region containing both the target sites was amplified by specific  primers with overhang Illumina adapters (Table 2). PCR reaction was performed using a mix containing 12.5 l of PCRBIO HS Taq Mix Red (PCRBiosystems), 0.4 M of each primer, 25 ng of DNA template in a final volume of 25 l. Amplification conditions were: 1 cycle at 95C for 5 min, 33 cycles at 95C for 30 sec, 55C for 30 sec, 72C for 30 sec and a final cycle at 72C for 5 min