RNA-seq of homo sapiens: peripheral CD4+ central memory T cells (early treated HIV-1)
RNA was subjected to quality control with Agilent Bioanalyzer (Agilent) and cDNA was prepared using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara). The produced cDNA quality was examined on Agilent TapeStation system using Qubit by (Thermo Fisher) and a High Sensitivity D5000 ScreenTape (Agilent). Libraries were then prepared with 1 ng cDNA using the Nextera XT DNA Library Preparation Kit (Illumina). The yield and quality of the amplified libraries were analysed using Qubit by (Thermo Fisher) and the Agilent Tapestation (Agilent). The indexed cDNA libraries were normalised and combined; the pools were sequenced on the Illumina Novaseq 6000 S4 flowcell for a 150 cycle paired-end sequencing run generating 150 bp paired-end reads.