NextSeq 500 sequencing; RNA-seq of doxycycline induced tumor and residual mouse primary mammary epithelial cells grown in 3D cell cultures against non-induced controls
RNA-seq of doxycycline induced tumor and residual mouse primary mammary epithelial cells grown in 3D cell cultures against non-induced controls
The cells for the RNA extraction were harvested from a pool of two 3D gels per experimental condition using 900 ?L of mirVana lysis buffer. "Cells grown in Lonza MEBM serum free media (CC-3151) "The library was sequenced in the Genomics Core Facility at EMBL Heidelberg on an Illumina HiSeq 4000 platform generating strand-specific single-end reads with a read length of 75 bp. The samples 208_CtrlNIT0 and 208_Tum were multiplexed together on one lane  as well as the samples 271_CtrlNIT0 and 271_Tum. In both cases the samples were multiplexed alongside with 8 other equivalent samples. The samples 208_Res and 271_Res were separately multiplexed together with 10 other equivalent samples on one lane." "The RNA extraction was done using a mirVana miRNA Isolation Kit with phenol (Ambion  AM1560). The RNA quality and concentration was assessed with an Agilent 2100 Bioanalyzer (G2939BA)."