GSM4730050: WTD4_2; Mus musculus; OTHER
Genomic DNA was extracted from day 4 cultured B cells. Libraries were prepared according to protocol in (Hu J, et al. Nat Protoc 11:853–871.(2016)) Linear amplifications were performed using Sμ-specific biotin primer  5′/5BiosG/CAGACCTGGGAATGTATGGT3′) and followed by nesting PCR with (5′CACACAAAGACTCTGGACCTC3′)  AflII is used to remove germ-line sequence. high-throughput genome translocation sequencing (HTGTS). Sequenced with MiSeq 500v