GSM5553235: 9_2_S90_R1_001; Homo sapiens; RNA-Seq
Frozen samples were immersed in RLT buffer (AllPrep DNA/RNA/miRNA Univers al Kit, Qiagen) and disrupted and homogenized using TissueLyser LT (Qiagen). Total RNA and DNA were isolated simultaneously from tissue lysates using the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen), following the manufacturer's instructions. The RNA and D NA concentration was measured using NanoDrop 2000 (Thermo Scientific) and Qubit fluorometer (Thermo Fisher Scientific Libraries were prepared using Kapa mRNA HyperPrep, sequencing was performed using Illumina HiSeq (Single Read, 50 bp)