GSM5955905: Naïve ESC- ATAC_rep1; Mus musculus; ATAC-seq
RNA was extracted using Trizol. For ChIP cells were  lysed in RIPA buffer as described in Mousavi et al., 2012. ATAC-seq was performed according to a published protocol (Buenrostro et al., 2013) For RNA-seq, poly (A)+ mRNA libraries were generated  using NEBNext Ultra II RNA library preparation kit for Illumina (NEB #E7490). Single-cell RNA-seq libraries were prepared using a Chromium Single Cell 3' Library & Gel Bead Kit v3.1 (P/N 1000121, 10x Genomics) and scATAC seq performed using Chromium Next GEM Single Cell ATAC Library & Gel Bead Kit v1.1 (10x genomics, 1000175). Single Cell Multiome ATAC+ Gene Expression (10x Genomics kit, CG000338). Hi-C experiments were performed using the Arima-HiC kit (A510008GFP) Bulk RNAseq, Bulk ATAC seq, ChIPseq, scRNA, scATAC, snRNA and scATAC from the same cell (multiomeic) and HiC