GSM4826154: noPRP-2; Oryctolagus cuniculus; RNA-Seq
After isolation of total RNA by total RNA TRIzol® Reagent (Invitrogen; Carlsbad, California, USA), mRNA was purified by magnetic beads, digested into short fragments, and then reverse transcribed into double-stranded cDNAs. A sequencing library was constructed with Illumina TruseqTM RNA sample prep Kit (Illumina; San Diego, California, USA) and sequenced on an Illumina Hiseq platform.