PacBio RSII CLR sequencing of Manihot esculenta cv. AM560-2
Rebecca Bart's group at the Donald Danforth Plant Science Center grew and collected etiolated AM560-2 leaves, from which high molecular weight (HMW) genomic DNA was extracted into low-melting agarose plugs by Julia Vrebalov at the Boyce Thompson Institute. Jessica B. Lyons (UC Berkeley) also extracted HMW DNA from fresh leaf tissue using the CTAB method. These DNAs were sent to the University of California, Davis DNA Technologies & Expression Analysis Core for sequencing with PacBio RSII machines using P6-C4 chemistry.