GSM5625155: Null_Crown_50DAS_HP_1; Triticum aestivum; RNA-Seq
Total RNA was extracted using TRizol reagent (Invitrogen) according to the manufacturer's instructions. Following quantitation with a NanoDrop (Wilmington, DE), 15 µg of total-RNA was used to isolate mRNA using Dynabeads™ Oligo(dT)25 (Invitrogen) following the manufacture's instructions. mRNA-seq Libraries were produced using the non-strand specific Brad's Rapid Ravi-seq procedure (Kumar et al. 2012).