GSM5518406: rna-N-D10; Homo sapiens; RNA-Seq
Total RNA was collected using Trizol (Invitrogen) followed by cleanup using RNA Clean and Concentrator (Zymo) using the manufacturer's protocol. Samples were then QCed using bioanalyzer and subjected to paired end sequencing (BGI platform). RNA-seq on Astrocyte biological replicates was performed using the Lexogen Quant-seq 3' mRNA-seq Library Prep Kit FWD for Illumina protocol (cat# 015.96). Double-stranded cDNA was synthesized and i7 adapters for Illumina sequencing were added during PCR amplification. RNA-seq libraries were sequenced on an Illumina HiSeq 4000 instrument at a depth of 30 million reads per sample.