GSM5552666: Patient Derived Xenograft model BCM-4913; Homo sapiens; Mus musculus; RNA-Seq
PDX tumor were harvested from mice and trimmed of excess fat pad tissue. Representative tumor fragments were snap frozen in liquid nitrogen. Tumor was homogenized and RNA extracted using the RNeasy Mini Kit from Qiagen. NuGEN Ovation RNA-Seq v2 Kit (protocol p/n 7102, reagent kit p/n 7102-08) along with ThermoFisher's ERCC RNA Spike-In Control Mixes Protocol (publication number 4455352, rev. D) Illumina TruSeq DNA-Seq library preparation (protocol p/n 15005180 Rev. C, June 2011, reagent kit p/n FC-121-2001) protocols