ITS sequences from long term maize monoculture plot sample 11
specific adapter sequence were ligated to each amplicon (NEBNext Ultra II DNA library prep kit). Following quantification, the samples were individually indexed (NEBNext Multiplex Oligos for illumina Dual Index Primers Set 1), and another AMPure XP bead based purification step was performed. Amplicons were then sequenced on Illuminas MiSeq platform, using a MiSeq v3 kit with 600 cycles