GSM5585199: MeT-5A NT, rep3; Homo sapiens; RNA-Seq
MeT-5A cells that have taken up FedEVs [CD63-GFP(+)] were isolated by FACS Melody (~7×105 cells).  Total RNA was isolated, using AurumTM Total RNA Mini Kit (BioRad) according to the manufacture`s protocol. RNA of 100 ng was used for the preparation of sequencing libraries with NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads (NEB) according to the manufacturer's instructions.