GSM6281282: H1975 cells, 328-shControl H4ac; Homo sapiens; ChIP-Seq
Cells were crosslinked for 10 min with 1% formaldehyde. After quenching and cell lysis, chromatin was sonicated to obtain chromatin fragment lengths of 100-to-1,000 bp. Indexed libraries were prepared using NEB Next DNA Library Prep (Illumina) and then sequenced using paired-end reads (75 bp) on the NextSeq500 platform (Illumina).