GSM5667183: WGBS data of DMS3-ZF line 133; Manihot esculenta; Bisulfite-Seq
Leaf tissues from 7-8 week-old plant were used for DNA extraction. For WGS, libraries were prepared from genomic DNA by using Kapa HyperPrep kit (Roche) with DNA UD indexes (IDT for Illumina) and sequenced on NovaSeq 6000 sequencer (Illumina). For WGBS, genomic DNA from samples were end-repaired and ligated with TruSeq LT DNA single adapters (Illumina) using Kapa DNA HyperPrep kit (Roche). Adapter-ligated DNA was converted with EpiTect Bisulfite Kit (Qiagen). Converted DNA was PCR-amplified by MyTaq polymerase (Bioline) for 12 cycles. Library quality and size were determined by D1000 ScreenTape (Agilent) and libraries were subsequently purified by AMPure XP beads (Beckman Coulter). Library concentrations were measured with Qubit dsDNA Broad-Range Assay kit (ThermoFisher). Libraries were sequenced on NovaSeq 6000 sequencer (Illumina).