GSM5699728: VA_23_S13: Melanoma2; Danio rerio; ncRNA-Seq
RNA extracts were collected from melanoma developing in adult zebrafish or from EVs isolated from the same tumors. Total RNA was isolated using Single Cell RNA Purification Kit (Norgen) following manufacturer's instructions, subjected to DNase-I (Thermo Fisher Scientific) treatment and subsequently purified using phenol-chloroform Libraries were constructed using Clontech SMARTer smRNA-Seq kit. Libraries were sequenced on the Illumina HiSeq 2500 following the manufacturer's protocols.