GSM5699786: Control; Mus musculus; RNA-Seq
hearts were harvest, minced, washed with cold PBS, digested with collagenase to get single cell suspension. The cells were stained with anti-CD45 antibody for FACS sorting. Dead cells were excluded by live-dead dye staining. Single cells were encapsulated into emulsion droplets using Chromium controller (10X genomics). The scRNA seq libraries were prepared using the chromium single cells 3' regent kit (V3) (10X genomics) according to the manufacturer's protocol. scRNA-seq libraries were evaluated and quantified by Agilent Bioanalyzer using High sensitivity DNA kit and Qubit. Individual libraries were diluted and pooled for sequencing. Pooled libraries were sequenced with Illumina NovaSeq platform. single cell RNA-seq