GSM5396805: M2_r3; Homo sapiens; RNA-Seq
Total RNA was extracted from the cells using the RNeasy Mini Kit (Qiagen, Valencia, CA) in 3 replicates per group. RNA-seq libraries were generated using TruSeq Stranded mRNA kit from Illumina and single-end sequenced as 50 nt reads using HiSeq3000 (Illumina) at the Finnish Functional Genomics Centre (Turku, Finland). The libraries were sequenced twice; in the second run the library pooling was adjusted to account for unbalanced yield of the first run.