NC1
The DNA libraries were prepared according to the Illumina sequencer compatible protocol. First, the extracted DNA was fragmented using the Covaris S-series (Covaris, Woburn, MA, USA) focused ultrasonicator using the manufacturers protocol. The fragmented DNA was then size selected from 200 to 500 bp with the SPRI bead-based size selection protocol. According to the manufacturers instructions, genomic DNA was bisulfite-treated using the MethylCode Bisulfite Conversion Kit (Life technologies). The qualified libraries were diluted and loaded on the Illumina HiSeq X Ten platform, and 150 bp paired-end reads were produced.