GSM5050665: Case2_RNA; Homo sapiens; RNA-Seq
CBMC aliquots were thawed in a 37˚C water bath and resuspended in RPMI-1640 with 10% FBS (Thermo Fisher). Samples were centrifuged at 350 x g for 7 minutes at 4˚C. Cells were resuspended in 100 microliters of 1X PBS with 2.5% FBS and 2 mM EDTA. Dead cells and red blood cells were depleted using the EasySep Dead Cell Depletion Kit and EasySep RBC Depletion Reagent (Stem Cell), according to manufacturer instructions. Cells were resuspended in RPMI/10% FBS and counted. Cells were loaded on to the 10X Chromium controller at a targeted recovery density of 10,000 cells per sample. Samples were processed and sequencing libraries were created using the 10X the Chromium Next GEM single-cell V(D)J Reagent Kit v1.1 with human TCR V(D)J Enrichment following manufacturer instructions. scRNA-seq