GSM5912860: MIAMI IFNg_rep3; Homo sapiens; RNA-Seq
Following the treatments, medium was aspirated from cells, and cells were detached from wells by RNAzol and stored at − 80 °C until RNA isolation. RNA was purified using RNAzol according to the manufacturer's instructions.  Illumina TruSeq Stranded Total RNA kit was used for library preparation. 150 base paired-end read sequencing was performed using Illumina NextSeq500. All procedures were performed according to the manufacturer's instructions. Quality control assessment was done using Illumina RNAseq pipeline to estimate genomic coverage, percent alignment, and nucleotide quality. Raw sequencing data were transformed to fastq format. 