GSM5310233: WT liver (mouse D); Mus musculus; RNA-Seq
Mouse organs were collected immediately upon sacrifice, washed in ice-cold PBS, placed in ~4 volumes of Trizol (Invitrogen), and then sonicated using a hand-held sonicator (OMNI International) with a few brief pulses until there were no more visible chunks. Samples were stored at -80ºC until use. Libraries were prepared using the SMARTer Stranded Total RNA-Seq Kit v2 (Takara) per manufacturer's instructions and sequenced on a NovaSeq6000 with 100 bp single-end reads.