GSM5898061: BM, CD34+HSPCs, ITP, 2, 10X; Homo sapiens; RNA-Seq
BM samples were obtained by iliac crest aspiration, and removed erythrocytes. BM CD34+ HSPCs were puriﬁed using fluorescence-activated cell sorting (FACS) and the cellular suspensions were loaded onto a Chromium Single Cell Chip (10X Genomics) with barcoded gel beads at a target capture rate of ~6,000 individual cells per sample. The scRNA-seq dataset of each sample were produced with a Chromium system (10X Genomics, PN1000073) following the manufacturer's instructions.