GM12878 Unsheared UNCALLED Raw Signal
Prepared using the ONT Ligation Sequencing Kit (SQK-LSK109) without the DNA Control Strand (DCS) or FFPE repair in the end prep step. The initial sample volume was thus adjusted to 50ul, and Ultra II End Prep Reaction Buffer volume was adjusted to 7ul. ONT MinION flowcells (FLO-MIN106) with vR9.4.1 pores were used for sequencing. Nuclease flush Buffer A was prepared by combining 659ul of ultra pure water, 300 ul of 1M KCl, 30ul of pH 8.0 HEPES buffer, 10ul of 1M MgCl2, and 1ul of 2M CaCl, and two nuclease flushes were peformed after 24 and 48 hours of sequencing.