WT_CL
Sequencing libraries were prepared from 100 ng of total RNA using the TruSeq Stranded Total RNA sample preparation kit (Illumina) according to the manufacturer's protocol. Briefly, mRNA was purified, fragmented and used for first- and second-strand cDNA synthesis followed by adenylation of 3 ends. Samples were ligated to unique adaptors and subjected to PCR amplification. Libraries were then validated using the 2100 BioAnalyzer (Agilent), normalized and pooled for sequencing. RNA-Seq libraries prepared from two replicates for each experimental condition were subjected to single-ended sequencing on the Illumina HiSeq 4000 using barcoded multiplexing and a 90-bp read length. Image analysis and base calling were done with Illumina software