GSM4731362: cancercell_treatment-resistant macrophage-depleted_replicate4; Mus musculus; RNA-Seq
Cancer cells (CD45-iRFP+ cells) from prostate cancer bone metastasis with different treatments were sorted by flow cytometry. Cancer cells from 3 mice were pooled together for total RNA extraction using RNAeasy micro plus kit (Qiagen, Catalog # 74034) following the manufacturer's instructions. The quality control of samples was performed using bioanalyser picokit (Agilent Technologies). mRNAs were enriched using oligo dT magnetic beads, fragmented, and converted into a library of cDNA fragments. The cDNAs were then purified and amplified by PCR using ABI StepOnePlus Real-Time PCR System. The constructed cDNA library was quality checked by Agilent 2100 Bioanalyzer. 2 x 150 bp paired end sequencing was performed using BGISEQ-500 following the manufacturer's instructions.