16S rRNA sequencing of Bos taurus: pelleted fraction of milk bulk tank sample
The V4 hypervariable region of the bacterial 16S rRNA gene (292 bp fragment length) was amplified using the 515F (5GTGCCAGCMGCCGCGGTAA-3) and 806R (5- GGACTACHVGGGTWTCTAAT -3) primer set (Walters et al., 2016). Amplification conditions were set according to the proprietary procedure set by Novogene.