Illumina NovaSeq 6000 paired end sequencing
Following ribosomal RNA depletion (NEBNext, E6350) from 250 ng total RNA, sequencing libraries were constructed using the NEBNext Ultra II Directional Library prep kit for Illumina (E7760S), followed by Quality Control (Agilent D5000 Screen Tape System) and quantification (Qubit dsDNA high sensitivity assay kit (Thermofisher, Q32854)