GSM5518304: GC-Po-KdNc2; Gallus gallus; RNA-Seq
Total RNA was extracted from primary granulosa cells using TRIzol reagent (Invitrogen, USA) following the manufacturer's protocol. The sequencing library was generated under the instruction manual with NEB Next UltraTM RNA Library Prep Kit for Illumina (NEB, USA). Obtained cDNA libraries were sequenced on an Illumina Nova platform and generated paired-end reads.