GSM5464807: molnc301b_IP_even; Homo sapiens; ChIP-Seq
About 2×107 K562 cells were crosslinked by 1% glutaraldehyde followed by sonication. Lysates were clarified from sonicated chromatin and RNA-DNA complexes were isolated by antisense biotinylated oligos on streptavidin beads according to the protocol published by Chu et al., 2011. Then the DNA was purified for subsquential processes. Libraries were prepared according to NEB's instructions accompanying the NEBNext Ultra II DNA Library Prep Kit (E7103S) and NEBNext Multiplex Oligos for Illumina (E7335S).