GSM5006428: decidualized endometrial stromal cells (dESCs), si-SCRIB, rep2; Homo sapiens; RNA-Seq
Total RNA was extracted from decidualized ESCs transfected with si-Ctrl and si-SCRIB using the TRIzol reagent (Invitrogen, USA). Isolated RNA was quantified by the NanoDrop 2000 spectrophotometer (Thermo Scientific, USA), and the RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, USA). RNA libraries were prepared for sequencing using standard Illumina protocols