RNA-seq of Drosophila melanogaster: Female midgut from virgin flies
Female guts were collected two days after mating to SP0 or SPWT males, and from virgin females. RNA was isolated from 60 guts per sample per replicate, using a modified TRizol extraction protocol (Life Technologies). Following RNA extraction, 3end RNA-seq libraries were prepared using QuantSeq 3 mRNA-Seq library prep kit, according to manufacturers instructions (Lexogen).