Shiga toxin-producing Escherichia coli
The Wizard Genomic DNA Purification kit (Promega, Madison, WI, USA) was used to prepare DNA. Libraries for single-molecule real-time (SMRT) sequencing were constructed with an insert size of 10 kb using the SMRT bell TM Template kit version 1.1. SMRT sequencing was performed using PacBio Sequel platform (Pacific Biosciences, Menlo Park, CA, USA).