TCRa deep sequencing a WT C57BL/6 mouse thymus
CD4+/CD8+, TCRb-, MR1 5-OP-RU tetrramer-, CD1d aGalCer tetramer- thymocytes (3.5x10^6) were bulk sorted and total RNA isolated using the ISOLATE II RNA Minikit (Bioline). cDNA libraries were prepared using the SMARTer Mouse TCR a/b Profiling kit (Takara Bio).