GSM5961441: Safe NoTNF and Safe TNF; Homo sapiens; RNA-Seq
RNA was extracted using the Qiagen RNeasy kit Libraries were prepared using a plate based in-house library prep method based on DRUG-seq (Ye et al., 2018). The method results in 3' RNA-seq libraries containing a UMI and a well ID on read 1, and the unique transcript information on read 2. Libraries were sequenced on the NextSeq500 using the 75bp single end chemistry, run with paired-end settings. 25bps was allocated for the Read1 and 50bps for Read