GSM5857145: WT_2; Mus musculus; RNA-Seq
For bulk RNA-seq in skin ILC, single cell suspension were obtained from the skin of  RORγt-fm and RORγt-fmΔGata3 mice, C57BL/6 and Gata3fl/flCreERT2 mice. Cells were sorted by a BD Aria III cell sorter for live Lineage-γδTCR-CD90+. For scRNA-seq, skin ILC were sorted from ear and dorsal skin on Day 0 and Day 10 after depilation. RNA libraries were prepared for sequencing using standard Illumina protocols