GSM5172086: HSM2_T5_R2; Homo sapiens; RNA-Seq
Total RNA was harvested in Trizol and stored at -80˚C until Quant-seq analysis. The Library was made using Lexogen QuantSeq 3' mRNA-Seq Library Prep Kit FWD according to the manufacturer's instructions, which was then sequenced on Illumina HiSeq 3000. Sequencing was by the MHTP Medical Genomics Facility and applied Illumina directional RNA-seq on the Illumina Hiseq3000 using 150bp forward read. Libraries were purifified using AMPureXp beads and QC'd by Qubit, Bioanalyzer and qPCR. Mean size 343bp; 7.8nM. [Cluster Generation]  c-bot clustering using 200pM of library pool. Illumina Protocol 15006165 v02 Jan 2016 [Sequencing Chemistry]  PE sequencing using Illumina HiSeq 3000. Illumina Protocol 15066493 Rev A, February 2015