GSM5353720: WTB Wild-Type replicate 1; Homo sapiens; RNA-Seq
Total RNA was purified from iPS-CMs by using TRIzol and PureLink RNA Mini Kit (Thermo Fisher Scientific) according manufactures' instructions. The RNAseq libraries were prepared from the purified RNA by using TruSeq RNA Library Prep Kit v2 (Illumina). Paired-end 100 bp RNAseq was performed with HiSeq (Illumina). An average of ~90 million reads were obtained from this iPS-cell RNA-Seq data (45-182 million PE reads).