GSM6249182: Batf2 KO HSC replicate 1; Mus musculus; RNA-Seq
Leg and hip bones were isolated and HSCs were purified via FACS sorting following mechanical isolation and c-kit+ magnetic cell enrichment. DNA and RNA were isolated with the RNeasy Kit (Qiagen, Cat#74004). RNA-seq libraries were prepared using SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara Bio Usa). RNA libraries were prepared for sequencing using standard Illumina protocols