GSM5269421: G2333_Pph_BR1; Phaseolus vulgaris; RNA-Seq
Total RNA was extracted with the TRIzol reagent (Thermo Fisher Scientific, Rockford, IL) following the manufacturer's recommendations. RNA was quantified using a NanoDrop spectrophotometer (Thermo Fisher Scientific, Rockford, IL). RNA quality was evaluated with a BioAnalyzer 2100 (Agilent, Santa Clara, CA). RNA-seq libraries were prepared with 2 µg of total RNA with the TruSeq RNA v2 kit (Illumina, Inc., San Diego, CA), according to the manufacturer's protocol. The concentration of the libraries was measured with the Qubit fluorometer (Invitrogen, Carlsbad, CA) and fragment sizes were determined using the Agilent High Sensitivity DNA kit on a BioAnalyzer 2100 (Agilent Technologies, Waldbronn, Germany). RNA sequences (1 × 100 bases) were obtained with a HiSeq 2000 system (Illumina Inc., San Diego, CA) at the DNA Core Facility, University of Missouri, USA.