HiSeq X Ten paired end sequencing; Genetic stability and germ cell epigenetic instability in the Mtrr mouse model of transgenerational epigenetic inheritance.
Genetic stability and germ cell epigenetic instability in the Mtrr mouse model of transgenerational epigenetic inheritance.
All conceptuses were collected at E10.5. Noon on the day the vaginal plug was detected was considered E0.5. Embryos were scored for phenotypes as previously published (Padmanabhan et al., 2013) DNA was extracted from tissues using the DNeasy Blood & Tissue Kit (Qiagen), according to manufacturerâ€™s instructions. Samples were sent to the BGI, HongKong, who perfomred library construction using 3ug of DNA