GSM5960905: WM_1245_4; Bombus lucorum; RNA-Seq
Head and gut of the bumbleebees were disected in the field and afterwards the thorax and abdomen was frozen at -80°C. RNA was extracted using the RNeasy Mini Kit according to manufactur protocol. RNA quality was checked using a 2100 Bioanalyzer with the RNA 6000 Nano kit (Agilent Technologies). The RIN for the samples was from 2,7 – 9,0. DNA libraries suitable for sequencing were prepared from 400 ng of total RNA with oligo-dT capture beads for poly-A-mRNA enrichment using the TruSeq Stranded mRNA Library Preparation Kit (Illumina) according to manufacturer's instructions (½ volume, 4 samples: full volume). After 15 cycles of PCR amplification, the size distribution of the barcoded DNA libraries was estimated ~285 bp by electrophoresis on Agilent DNA 1000 Bioanalyzer microfluidic chips.