WGS of Culicoides sp. (close to C. tainanus)
Whole Genome DNA libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina. Briefly, around 20 ng of DNA was used for library preparation. DNA was sheared using Adaptive Focused Acoustic technology (Covaris, Inc.), and fragments of length around 200 bp were generated. The fragments were end repaired, 3-adenylated, ligated with adapters. Later, PCR enrichment was carried out using Illumina sequencing indexes. The size selection was done using solid-phase reversible immobilization (SPRI) beads from Beckman Coulter (Agencourt AMPure XP Beads)