GSM5464311: TBX3-KO-D12-Hep-2-resub [ATAC-seq]; Homo sapiens; ATAC-seq
Cells were lysed and nuclei were treated with Tn5 enzyme (Illumina, Cat. #20034197) for 30 minutes at 37°C. DNA was purified with Minelute PCR Purification Kit (Qiagen, Cat. #28004) to produce tagmented DNA samples. Tagmented DNA was barcoded with Nextera Index Kit v2 (Illumina, Cat. #FC-131-2001) and libraries were made for sequencing following standard protocols from Illumina