Illumina NovaSeq 6000 sequencing; TDP-43 iCLIP of SHSY5Y cells
TDP-43 iCLIP of SHSY5Y cells
SHSY5Y cells were crosslinked and pelleted following the standard iCLIP protocol. The antibody used was Proteintech, Rabbit anti-TDP-43 Cat#10782-2-AP RNA for library preparation was enriched by TDP-43 antibody pulldown Standard iCLIP protocol (3' adapter ligation, RT, circularisation, PCR)