Illumina HiSeq 2000 sequencing; ChIP-seq analysis with an antibody directed against the human Rad21 protein in Raji cells (Genbank: KF717093.1) prior to and post induction of BZLF1.
ChIP-seq analysis with an antibody directed against the human Rad21 protein in Raji cells (Genbank: KF717093.1) prior to and post induction of BZLF1.
Chromatin IPs were conducted with an antibody directed against Rad21 (Abcam, #992) comparable to ChIP experiments described in Woellmer et al (2012) (Woellmer A, Arteaga-Salas JM, Hammerschmidt W (2012) BZLF1 governs CpG-methylated chromatin of Epstein-Barr virus reversing epigenetic repression. PLoS Pathog 8:e1002902. doi: 10.1371/journal.ppat.1002902). DNA fragments were isolated in principle as described in Woellmer et al (2012) (Woellmer A, Arteaga-Salas JM, Hammerschmidt W (2012) BZLF1 governs CpG-methylated chromatin of Epstein-Barr virus reversing epigenetic repression. PLoS Pathog 8:e1002902. doi: 10.1371/journal.ppat.1002902). The library construction was performed by the company Vertis Biotechnology AG (Freising, Germany) using TrueSeq sequencing adaptors and DNA fragments of 300 to 500 nt in length.