Genomic sequencing of SARS-CoV-2: wastewater
RNA extracted from wastewater samples is converted to cDNA using LunaScript RT SuperMix (NEB). Tiled amplicons are generated and prepared for sequencing using the Normalase SARS-CoV-2 Amplicon Panel (Swift Biosciences) following manufacturers instructions. Pooled libraries were sequenced on an Illumina MiSeq.