Illumina MiSeq paired end sequencing
V4 16S rRNA using primers 519F and 806R
DNA extracts were  prepared for paired-end MiSeq sequencing (Illumina, CA, USA) using a two-step PCR method to amplify the V4 hypervariable region of bacterial and archaeal 16S rRNA genes, and to attach MiSeq adapter and barcode sequences.