GSM5298148: PMDK002F_S6: Crh1KO_Rep3; Caenorhabditis elegans; RNA-Seq
1-day adult worms were collected using Trizol LS reagent. Total RNA was extracted via isopropanol precipitation and treated with Turbo DNase (AM2238) according to manufacturer instructions. RNA quality was assessed by Bioanalyzer (Agilent) prior to library construction. Illumina TruSeq Stranded Total RNA Library Prep Gold kit (Cat #20020598) was used with 1ug of total RNA to construct sequencing libraries