GSM5857923: AAV8-STAT5ca-treated Male-2 [G169_M30]; Mus musculus; RNA-Seq
Trizol was ued to isolate total liver RNA from flash fozen mouse liver tissue. Polyadenylated mRNA was isolated from 1 μg of liver nuclear RNA using NEBNext® Poly(A) mRNA Magnetic Isolation Module (cat. #E7490, New England Biolabs) and used for lllumina RNA-seq library preparation using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (cat. #E7420L, New England Biolabs), NEBNext® Multiplex Oligos for Illumina® Dual Index Primers Set 1 (cat. #E7600, New England Biolabs), and AMPure® XP Beads (cat. #A63881, Beckman Coulter Inc., Indianapolis, IN) per the manufacturer's instructions.