WES_Homo_sapiens_HNSCC
Genomic DNA was sheared with Covaris followed by end-repair, 3 end Adenylation and ligation with paired-end adaptors. Purified libraries were PCR amplified and every step was followed by DNA purification using AMPure beads. Size and quantity were verified employing the Agilent Tapestation 2200 system. Hybridization to the capture library was performed using Exome SureSelect probes (homo sapiens), Streptavidin magnetic beads (Thermofisher), were resuspended in SureSelect Binding buffer to which the Hybridized Exome Captured libraries were added and mixed (Agilent). Finally, Captured Libraries were amplified using indexing primers, followed by an amplification step. Final libraries were checked for quality (each fragment size approx. 300-400 bp) and quantity using Agilent Tapestation.