RNA-seq acute myeloid leukemia
The samples were obtained from 5 children aged between 2 and 12 years, newly diagnosed with ALL . All patients were referred to Dr. Sheikh Hospital of Mashhad, Iran, between February 2019 and September 2019. Bone marrow mononuclear cells (BM MNCs) were isolated according to standardized protocols by Ficoll (Biowest, France) which allowed mononuclear cells separation according to a density gradient. Subsequently, total RNA isolation was carried out using the Tripure reagent (Roche, Mannheim, Germany) according to manufacturers guidelines. To assure the high quality of isolated RNA for next generation sequencing, DNA contamination was prevented by DNase I (Thermo Fisher Scientic, Waltham, MA, USA) treatment. In the next step, paired-end RNA-sequencing was performed with NovaSeq6000 platform (Illumina) by CeGaT company (Tubingen, Germany).