GSM5585245: Ecoli - PCR; Escherichia coli; OTHER
Genomic DNA was extrated using DNeasy Blood and Tissue Kit according to the manufacturer's instructions (Qiagen, 69506) Genomic DNA was fragmentized to 100-300 bp using Covaris S220 Focused-ultrasonicator. Fragmented gDNA was ligated to TruSeq adaptor using NEBNext Ultra™ II DNA Library Prep Kit for Illumina (NEB, E7645S). Unmodified control DNA was made by amplifying adaptor-ligated DNA using Q5 DNA polymerase (NEB, M0492S). Nitrite treatment was performed in both native and amplified DNA. Indexed libraries were constructed using NEB ultra ii library prep kit.