GSM5912788: 7- CD45.1 WT infected (no transplant) replicate 2- TITR 3; Mus musculus; RNA-Seq
Leg bones were isolated and HSCs were purified via FACS sorting following mechanical isolation and c-kit+ magnetic cell enrichment. DNA and RNA were isolated with the NucleoSpin ® RNA Plus XS kit (Macherey Nagel). RNA-seq libraries were prepared using SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara Bio Usa). RNA libraries were prepared for sequencing using standard Illumina protocols