GSM5850369: ESC-HA_K27L_4_rep2-RNA; Mus musculus; RNA-Seq
Cells were flash frozen in liquid nitrogen, RNA was prepared using RNeasy (Qiagen) according to the manufacturer's instructions. Library preparation was done using QuantSeq 3′-mRNA-Seq Library Prep Kit (Lexogen) or KAPA mRNA-seq kit (Roche). Libraries were subjected to single-end sequencing using Illumina NextSeq500.