Illumina MiSeq paired end sequencing of SAMD00402084
DNA was extracted using Extrap Soil DNA Kit Plus ver.2 (NIPPON STEEL Eco-Tech Co., Ltd., Tokyo, Japan). The V4 region of 18S rRNA genes were amplified using TAReuk454FWD1and TAReukREV3 with KAPA 3G Plant PCR kit (Kapa Biosystems). The PCR products were purified by MonoFas DNA purification kit I (GL Sciences Inc., Tokyo, Japan) and sequencing library preparation was performed using Illumina Nextera XT Index Kit v2. The sequencing libraries were purified by NucleoMag NGS Clean-up and Size Select (Macherey-Nagel, D?ren, Germany).