Illumina HiSeq 4000 paired end sequencing; Bulk RNA-seq of the human fetal neocortex, lateral ganglionic eminence and medial ganglionic eminence at 7, 9, 11 and 20 post-conceptional weeks
Bulk RNA-seq of the human fetal neocortex, lateral ganglionic eminence and medial ganglionic eminence at 7, 9, 11 and 20 post-conceptional weeks
Embryonic and fetal age was extrapolated based on the date of the mother's last menstruation, ultrasound scans of the fetus in utero, crown-rump length (CRL) and visual inspection. Depending on the condition and period of the procured specimens, the neocortex, LGE and MGE were dissected. Both left and right brain regions of interest were collected from fresh tissues. Specimens were chilled on ice during dissection. The dissected samples were immediately frozen in dry ice and stored at −80 °C for later RNA extraction.  Total RNA was extracted from LGE, MGE and neocortical human fetal tissues with the TRIZOL reagent (ThermoFisher Scientific, 15596018) according to the manufacturer's instructions. After DNase treatment (ThermoFisher Scientific, AM1906), optical density values of extracted RNA were measured with NanoDrop (Thermo Scientific) to confirm an A260/A280 ratio ≥ 1.9. 500 ng of total RNA from each sample was used for the library preparation. unstranded, polyA enrichment