P2-PBMC-scRNAseq
PBMCs and PFMCs were subjected to 10 Genomics single-cell sequencing. Briefly, cell viability was assessed by trypan blue staining and the samples with viability >90% were prepared using a Chromium Single Cell Library Kit and Chromium Single Cell V(D)J Enrichment Kits, Human (10 Genomics).  The PBMCs and PFMCs were washed once with PBS containing 0.05% BSA and resuspended in PBS containing 0.05% BSA to a final concentration of 700-1,200 cells/l as determined by hemocytometer. Single cells were captured in droplets and subject to reverse transcription. Then the emulsions were broken, and the cDNA was purified before PCR amplification. Amplified cDNA was then purified by SPRI select and used for both gene expression library construction and TCR enrichment.