RNAseq of NIH3T3 cells overexpressing SRF-C3orf62
NIH-3T3 cells with stable integrations of SRF, C3orf62, SRF-C3orf62 or Nanoluc tagged at the C-terminus with EGFP were induced with 1 g/mL doxycycline for 24 hours. RNA was extracted from cells maintained in low-serum conditions (0.5% calf serum) for 22 hours using RNeasy purification kit (Qiagen) and treated with DNase on column. Samples were induced and collected in technical duplicates from 6-well plates. Libraries were prepared using the NEBNext Ultra II Directional RNA-seq with Poly-A selection kit, pooled and sequenced on a 100-cycle NovaSeq 6000 SP.