One step for hydrogen production by microbial consortium CV19
The DNA libraries were constructed through the processes of end repairing, adding A to tails, purification, PCR amplification and etc. After quantified by Qubit 2.0, the library was diluted to 2 ng/ul and the detected for the insert size by Agilent 2100. Q-PCR was performed to ensure the library quality (effective concentration > 3 nM). The flow cell was sequenced by Novogene Bioinformatics Technology Co. Ltd. (Beijing, China) using Illumina HiSeq2000 technology generating.