Illumina HiSeq 4000 sequencing; Molecular characterization of mucinous breast cancer by low-coverage WGS
Molecular characterization of mucinous breast cancer by low-coverage WGS
After approval of the ethics committee of the Institut Jules Bordet (Nr 2503, 04/02/2016), we assembled a cohort of samples consisting of histologically confirmed primary pure mucinous breast tumors from the Institut Jules Bordet Biobank. Non-invaded lymph nodes were considered when available as a source of germline DNA. For each sample, we centrally characterized the histo-pathological features and the percentage of tumor epithelial cells.  DNA was extracted using the QIAmp® DNA FFPE tissue kit (QIAGEN) following manufacturer's instructions.  Whole-genome DNA libraries were prepared with Illumina's TruSeq DNA sample preparation kit V2 according to the manufacturer's instructions.