GSM5857932: AAV8-STAT5ca-treated Male-5 [G186_M9]; Mus musculus; RNA-Seq
Nuclei were isolated from frozen liver tissue using methods detailed in Goldfarb CN, Waxman DJ. BMC Genomics. 2021;22(1):212. Trizol was used to isolate RNA from the purified liver nuclei. Polyadenylated mRNA was isolated from 1 μg of liver nuclear RNA using NEBNext® Poly(A) mRNA Magnetic Isolation Module (cat. #E7490, New England Biolabs) and used for lllumina RNA-seq library preparation using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (cat. #E7420L, New England Biolabs), NEBNext® Multiplex Oligos for Illumina® Dual Index Primers (cat. #E7600, New England Biolabs), and AMPure® XP Beads (cat. #A63881, Beckman Coulter Inc., Indianapolis, IN) per the manufacturer's instructions.