GSM4826557: AML21_bulk; Homo sapiens; RNA-Seq
RNAeasy Mini kit (Qiagen Inc.) Libraries were prepared using 150ng of RNA input and NEBNext® Ultra™ II Directional RNA Library Prep Kit (Illumina), following manufacturers' protocols. Sequencing of libraries was performed on an Illumina HiSeq 2500, high output flow cell (PE126bp) and targeted a final coverage of 30M paired reads per library.