Amplicon sequencing of diatom Pseudo-nitzschia assemblages: seawater biomass filters
Seawater plankton community biomass filters extracted for DNA and amplified with diatom genus Pseudo-nitzschia (NCBI:txid41953) inclusive primers with Illumina MiSeq adapters. In total, 144 samples were multiplexed using two unique sets of Illumina indeces on the same 2x250 bp Illumina MiSeq run at the University of Rhode Island Genomics & Sequencing Center. These reads have been demultiplexed and the Illumina MiSeq Nextera adapter sequences removed. No other processing steps have been taken. The forward primer used was ITS1 from White et al 1990: TCCGTAGGTGAACCTGCGG and the reverse primer used was custom designed for this study under Sterling et al: CATCCACCGCTGAAAGTTGTAA targeting a conserved region in the 5.8S rRNA gene of Pseudo-nitzshia species. Primers are reported from 5' to 3'.