GSM6329905: K562, HSP90 beta KO, replicate 1; Homo sapiens; RNA-Seq
RNA was isolated utilizing the Maxwell® RSC simplyRNA Cells Kit (Promega, #AS1390). Library preparation was carried out following supplier's guidelines by using the VAHTs Stranded mRNA-Seq Library Prep Kit (Illumina Inc. San Diego, USA). Briefly, total RNA (500 ng) was used for capturing of mRNA, fragmentation, cDNA synthesis, ligation of the adapters and library amplification. Purified libraries were normalized and sequenced on the NextSeq550 (Illumina) with 1x76 bp read setup. Followed by using bcl2fastq2 tool to convert the bcl files to fastq files.