GSM5699591: Adult E3; Anopheles gambiae; RNA-Seq
Total RNA was extracted from four pools of 20 individuals sampled for each life stage (larvae or adult females)  and condition (treated with pesticide mixture or not). RNA extractions were performed using Trizol (Life Technologies) according to manufacturer's instructions. RNAseq libraries were prepared from 150 ng total RNA using the NEBNext® Ultra ™ II Directional mRNA Library Preparation Kit for Illumina (New England Biolabs) following manufacturer's instructions. Libraries were quantified using the Qubit DNA BR assay (Thermofisher Scientific) and quality checked using Bioanalyzer DNA 1000 assay (Agilent). Libraries were sequenced in multiplex as single 75 bp reads on a NextSeq 500 sequencer (Illumina) by Helixio (Clermont-Ferrand, France). After unplexing and quality check using FastQC, reads