GSM5898193: H357 cells; Homo sapiens; RNA-Seq
Three cell lines. H357 KB and Hep-2 were maintained in DMEM-F12, DMEM and MEM growth media respectively, supplemented with 10% FBS and 1% antibiotics Pen-Strep at proper growing conditions. Total RNA of HOK (Cat. No. #2615) was purchased from Science cell research laboratories, USA. For H357, KB, and Hep-2, total RNA was extracted using trizol method. The purity of isolated RNA was evaluated using NanoDrop spectrophotometer (Thermo Scientific) by measuring absorbance at 260 nm and 280 nm. An aliquot of each sample from H357, HOK, KB and Hep-2 was run on an Agilent RNA Bioanalyzer to check RNA integrity number (RIN), which were found to be 10 for all the samples. The RNA libraries were constructed for sequencing following Illumina HiSeq transcriptome library protocol. 3' and 5' adapter ligation was done followed by PCR-amplification. After library construction, pair-end sequencing was done on Illumina Hiseq2500 with 2×150 bp to obtain reads. After the sequencing, RNA-Seq reads were quality checked and processed into FASTQ files.