GSM5960344: Wt-2; Mus musculus; RNA-Seq
We firstly crossed Tdfl/fl;Nex-Cre mice with Stop-tdtomato-stop to get Tdfl/+;Nex-Cre mice to distinguish excitatory neurons with Td (Fig.5a). The Td+ cell population from forebrain of P28 was isolated through fluorescence-activated cell sorting (FACS). We performed RNA sequencing (RNA-seq) of these Td+ cells.  RNA was harvested using Trizol reagent.RNA-seq libraries from WT and Arid1a+/-  at 28-day-old were prepared using Illumina RNA-Seq Preparation Kit.  Total RNA sample QC was measured by Agilent 2100 Bio analyzer (Agilent RNA 6001 Nano Kit) . RNA libraries were prepared for sequencing using standard Illumina protocols