Illumina GAIIx 104 bp Paired-end sequencing (2010) of Bradysia coprophila female embryo genomic DNA.
Genomic DNA was isolated from female embryos (Bradysia coprophila ; syn. Sciara coprophila) using DNAzol (ThermoFisher), sonicated to a size range of 100-600 bp, and prepared for Illumina sequencing using the NEBNext kit (New England Biolabs) following the manufacturers directions. The library was run on a 2% NuSieve agarose (Lonza) gel, size-selected near the 500 bp marker, gel purified (Qiagen), and sequenced on the Illumina GAIIx platform to obtain 104 bp paired-end reads.