GSM5651309: CKO for CUT&RUN using H3K4me1; Mus musculus; OTHER
Total RNA was isolated from samples using TRIzol reagent. For RNA-seq: VAHTSTM Stranded mRNA-seq Library Prep Kit for Illumina v2 was used for library construction. For CUT&RUN: The protein binding DNA was  isolated from cells using EpiTM CUT&RUN Assay Kit (Epibiotek, cat. no R1816). Extract DNA was processed for library generation using the QIAseq Ultralow Input Library Kit (QIAGEN) following the manufacturer's protocol. For ATAC-seq: The chromatin DNA was isolated from cells for library construction using EpiTM ATAC-seq kit(Epibiotek, cat. no EPI20180202) . For HiC: We used the HiC-pro pipeline (Servant et al. 2015) to process the Hi-C raw data.