GSM5464740: Cochlear organoid passage 4  3; Mus musculus; RNA-Seq
Organoids were washed by PBS for three times and harvested by centrifugation. Total RNA was extracted by trizol and flash frozen in liquid nitrogen. The RNA-sequencing libraries were generated by the Illumina mRNA-Seq Sample Prep Kit. fastq.gz files of paired-end read files were generated using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing on an Illumina HiSeq2500 150-bp Paired-End Platform.