GSM5899028: TLS-rich, Non-HEV-3; Homo sapiens; RNA-Seq
Total RNA was isolated using NucleoSpin® totalRNA FFPE (Clontech 740969.10) from tissues obtained by laser capture microdissection. An amount of 5 ng total RNA was used for constructing RNAseq library of each micro-dissected endothelium type from each tumor sample. RNA amplification from FFPE samples was carried out by Single Primer Isothermal Amplification method using NuGEN TRIO RNAseq Library Preparation kit (Part #0506) for library construction