GSM5356191: H1; Homo sapiens; RNA-Seq
To extract RNA we used Qiagen RNEasy mini kit and the quality of RNA tested with the RNA 6000 Nano Bio analyzer (Agilent Technologies, CA. USA). Transcriptome libraries were prepared using the Illumina TruSeq RNA library preparation kit (Illumina #RS-122-2001), according to the manufactures' recommendedprotocol, starting with approximately 1.2ug of total RNA. The amplified indexed libraries were quantified using Invitrogen Qubit fluorometer and equally pooled according to pool design