GSM5898213: WT-rep3; Saccharomyces cerevisiae; OTHER
Quick-RNA Fungal/Bacterial Kit (Zymo Research) as per manufacturer instructions with the modification of adding Reducing agent (RA) from the SLAM-Seq Kinetics Kit – Catabolic Kinetics Module (Lexogen) to the isolation, wash, and elution buffers of the RNA extraction kit at a ratio of 1:1000, 1:1000 and 1:100, respectively 200 ng of alkylated total RNA was used as input for generating 3'-end mRNA sequencing libraries using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen) and the and PCR Add-on Kit for Illumina (Lexogen) according to the QuantSeq 3' mRNA-Seq Library Prep Kit User Guide (Lexogen)