GSM6281259: LSD 8h rep3; Drosophila melanogaster; RNA-Seq
The larvae were homogenized with a disposable plastic pestle in lysis buffer. Total RNA was extracted using the Nucleospin RNA II kit (Macherey-Nagel) in accordance with the manufacturer's instructions. Elution was done with water, and the samples were stored at -80 °C. cDNA libraries were prepared from rRNA depleted samples using the TruSeq Stranded mRNA kit (Illumina). RNAseq (75 bp single end reads; reverse) was run on 2 technical replicates per sample with an average depth of 30 million reads per sample on the Illumina NextSeq500 using the 75bp cycle kit (Illumina).