RNA-Seq of mus lung: 11 days CCDC65+/-  mice lung
Total lung were removed, flash frozen on liquid nitrogen, and RNA was harvested using Trizol reagent.RNase was used to remove DNA and mRNA was enriched by magnetic bead contained Oligo(dT).The mRNA was broken into short fragments by adding interrupting reagent, and the mRNA was used as template to synthesize double-stranded cDNA by reverse transcriptional PCR.The double-stranded cDNA was amplified by PCR after purification, repair and selection and used for construction of sequencing libraries.