RNAseq of Pinus taeda: Arkansas provenance pooled families
From 8-week old greenhouse-grown seedlings, ten stems per family were harvested and pooled. The stems were flash frozen using liquid nitrogen to transfer to a -80C freezer and then freeze-dried prior to RNA extraction. Tissue was ground using a MiniG tissue homogenizer (SPEX Sample Prep, Metuchen, NJ) and 5/32-inch stainless steel balls. RNA was extracted using the RNAqueous-Micro Total RNA Isolation Kit (Ambion) and DNA was removed using the TURBO DNA-free kit (Ambion). The RNA was combined to create thirty pools (Suppl. Table 1, Suppl. Fig. 1), and libraries were prepared from each pool using the NEBNext Ultra RNA Library Prep Kit for Illumina. The thirty libraries were sequenced in five lanes of an Illumina NextSeq 500, with 150 cycles and paired-end format.