GSM5651366: HstAWCE_1; Haloferax volcanii DS2; ChIP-Seq
Cells were crosslinked and immunoprecipitated with HA antibody (ab9110) as described in Wilbanks et al 2012 (doi: 10.1093/nar/gks063) with the following changes to the protocol: the cross-linking reaction was allowed to proceed for 20 minutes rather than 10, and cell pellets were resupended in 800 μL lysis buffer instead of 1.6mL. Libraries were constructed using the KAPA Hyper Prep kit and Illumina TruSeq adapters according to manufactuer's instructions. DNA library quality was assessed by Bioanalyzer using a High Sensitivity DNA chip (Agilent) . Samples were pooled and run on an Illumina NovaSeq 6000 (Duke Sequencing and Genomics Technologies core) for paired-end sequencing.