GSM4826300: VNC WT_rep2; Drosophila melanogaster; RNA-Seq
Trizol extraction of total RNA from VNC was performed according to manufacturer's instructions. The TruSeq Stranded mRNA LT Sample Prep Kit was used for library construction. Libraries were evaluaded for quantification and quality using Agilent 2100 Analyzer and ABI StepOnePlus Real-Time PCR System and were sequenced on an Illumina NovaSeq 6000 sequencing platform (2 x 150bp). cycles.