Whole genome sequencing of Escherichia coli isolates from beef
The isolate was grown in LB  at 35oC for 18  2h. The DNA of each culture was extracted using  Lucigen Masterpure DNA/RNA kit following the manufactures instruction. The DNA samples were sent to Genome Quebec Innovation Centre, Montreal, QC, Canada for sequencing. Sequencing library was constructed using NEBNext Ultra II DNA Library Prep Kit and sequenced using Illumina HiSeqX PE (150bp)