GSM5912793: 11-CD45.2 trained infected (chimeric mice, transplanted) replicate 1 HSC; Mus musculus; RNA-Seq
Leg bones were isolated and HSCs were purified via FACS sorting following mechanical isolation and c-kit+ magnetic cell enrichment. DNA and RNA were isolated with the NucleoSpin ® RNA Plus XS kit (Macherey Nagel). RNA-seq libraries were prepared using SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara Bio Usa). RNA libraries were prepared for sequencing using standard Illumina protocols