GSM4824861: CONT-control; Bos taurus; RNA-Seq
Mononuclear cells were isolated from 2 g of mammary tissue after collagenase type IV and DNAse digestion. Cells were recovered through Percoll gradient centrifugation and stained with PE-labeled CD45 (CC1, BIO-RAD) and FITC-labeled CD4 (CC8, BIO-RAD) mAbs, before cell sorting using a MoFlo Astrios EQ (Beckman Coulter). Total RNA was extracted from samples with Nucleospin XS RNA kit (Macherey-Nagel). RNA libraries were prepared for sequencing using standard Illumina protocols