GSM5397338: PBS; Mus musculus; RNA-Seq
WT mice were injected with PBS or LCWE and two weeks later, the abdominal aorta (from the left renal artery to the aortic bifurcation) was collected on ice cold PBS. The PBS group was composed of a pool of 9 abdominal aortas, whereas the LCWE group was composed of a pool of 7 abdominal aortas. A cell suspension of the freshly collected abdominal aorta tissues was prepared with the gentleMACS OCTO Dissociator with Heaters (Miltenyi Biotech) using the program “37_m_TDK2” and the Multi Tissue Dissociation Kit 1 (Miltenyi Biotech), according to the manufacturer's instructions. The resulting cell suspension was washed in cold complete RPMI with 10% FBS and filtered through a 70μM cell strainer. For downstream application of scRNA-seq, dead cells were removed using the Dead Cell Removal Kit (Miltenyi Biotech) according to the manufacturer's instructions and viability was assessed with ViaStain AOPI staining solution (Nexcelom Bioscience) RNA libraries were prepared for sequencing using standard 10X Genomics Single Cell 3′ Reagent Kit (V3 chemistry)