GSM6248662: Huh-7, M2; Homo sapiens; RNA-Seq
The transfected cells were digested with trypsin and washed three times with PBS, and total RNA was harvested using Trizol reagent. Hieff NGS MaxUp Dual-mode mRNA Library Prep Kit for Illumina (Cat#12301ES96) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols