GSM6280746: RD-1_Ty1_3xTy1-TWIST2_2; Homo sapiens; ChIP-Seq
Cells were fixed with 4% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sheared on a Bioruptor Pico (Diagenode) for 20 cycles (30 sec on/ 30 sec off for each cycle) at 4°C ChIP-seq libraries were prepared using the NEBNext Ultra II DNA Library Preparation Kit complemented with NEBNext Multiplex Oligos for Illumina from NEB. 10 ng of DNA was used as starting material for input samples and 1 ng for ip samples. Libraries were amplified using 15 cycles for ip samples and 12 cycles for input on the thermocycler. Post amplification libraries were size selected at 250-450bp in length using SPRISelect beads from Beckman Coulter. Libraries were validated using the Agilent D1000 ScreenTape.