GSM5463941: CH3; Mus musculus; RNA-Seq
c-KitLow and c-KitHigh LT-HSCs from the BM of normal B6 and leukemic Scl-tTA-BCR-ABL mice were sorted, RNA extracted using RNeasy Plus Micro kit (Cat#74004, Qiagen, Valencia, CA), with four biological replicates from each group. Sequencing libraries were prepared with the SMARTer Ultra Low Input RNA Kit for Sequencing (v4, TaKaRa Clontech, catalog no. 634891) and Nextera XT DNA Library Preparation Kit (96 samples, Illumina, cat no. FC-131-1096). Sequencing was performed using the HiSeq 2500 platform with the HiSeq SBS Kit V4 (Illumina). STAR (version 2.5.3a) was used to align raw RNA-Seq FASTQ reads to the mouse reference genome (Gencode Release M11), and number of reads mapping to each gene enumerated using HTSeq-count50.