GSM5101892: 15183_HM; Macaca mulatta; RNA-Seq
Single cell suspensions were made using a gentleMACS Octo (MiltenyiBiotec). Single-cell suspensions were loaded onto the Chromium Controller (10x Genomics) for droplet formation. scRNA-seq libraries were prepared using the Chromium Single Cell 3' Reagent Kit (10x Genomics). The cell suspension (300-600 living cells/ml) was loaded onto the Chromium single cell controller (10x Genomics) by using single cell 3 'Library and Gel Bead Kit V3 (10x Genomics, 1000075) and Chromium Single Cell B Chip Kit (10x Genomics, 1000074). scRNA-seq