Whole exome seq of sorted KIT+ cells from ACC PDX model ACCX11
Genomic DNA was extracted using the PureLink Genomic DNA Mini Kit (Invitrogen K1820-01, Lot 2285292).  Genomic DNA samples were randomly fragmented by sonication to the size of 180-280 bp fragments. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities and enzymes were removed. After adenylation of 3' ends of DNA fragments, adapter oligonucleotides were ligated. DNA fragments with ligated adapter molecules on both ends were selectively enriched in a PCR reaction. After PCR reaction, the libraries were hybridized in buffer with biotin-labeled probes, then magnetic beads with streptomycin were used to capture the exons. Exome capture was performed using the Agilent SureSelect Human All Exome V6 (58 M). After washing beads and digesting probes, the captured libraries were enriched in a PCR reaction to add index tags. Products were purified with AMPure XP system (Beckman Coulter, Beverly, USA) and quantified by using the Agilent high sensitivity DNA assay on the Agilent Bioanalyzer 2100 system. BWA (v0.7.17) was used to map sequencing reads and output the alignment file in bam format. 