Uninfected macrophages+2DG
Library was prepared by using NEBNext Ultra Directional RNA Library Prep kit. During second cDNA synthesis, dUTP was incorporated to maintain strand specificity. The isolated polyA RNA or rRNA/globin depleted RNA was Mg2+/heat fragmented (200 bp), reverse transcribed to 1st strand cDNA, followed by 2nd strand cDNA synthesis labeled with dUTP.