GSM6246450: muscle sample of Eriocheir sinensis in pre-molt stage [Emu_D_2_RNA-seq]; Eriocheir sinensis; RNA-Seq
total RNA was extracted using the TRIzol method (Invitrogen) for RNA-seq analysis. For RNA-seq analysis, RNA integrity was assessed using the RNA Nano 6000 Assay Kit of a Bioanalyzer 2100 system (Agilent Technologies, CA, USA). A total amount of 1 μg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using a NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer's recommendations. Library quality was assessed on the Agilent Bioanalyzer 2100 system and 150 bp paired-end reads were generated.