RNA-Seq of Aedes polynesiensis: adult male
Total RNA was isolated from single adult male mosquitoes using the TRIzolTM Plus RNA Purification Kit and PhasemakerTM Tubes Complete System (Invitrogen Life Technologies, USA) according to the manufacturers protocol.  Following elution, each RNA sample was treated with the DNA-free Kit TURBO DNase (Invitrogen Life Technologies, USA) according to the manufactures Rigorous DNase Treatment protocol. Polyadenylated mRNA was enriched from the total RNA samples using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England BioLabs, Ipswich MA, USA). Double stranded cDNA libraries were created from enriched mRNA using the Illumina TruSeq Stranded mRNA library preparation kit (Illumina, San Diego, CA, USA) . Libraries were sequenced with 75 x 75 paired-end sequencing on the Illumina MiSeq platform at Smith College (Northampton, MA, USA).