GSM4826812: iPS microglia PBS_rep 3; Homo sapiens; RNA-Seq
Extraction of total RNA was performed according to the manufacturer's instructions (RNeasy, QIAGEN) 10 ng of total RNA was reverse-transcribed using the SuperScript VILO cDNA Synthesis Kit (Thermo Fisher Scientific) followed by library generation using the Ion AmpliSeq Transcriptome Human Gene Expression Kit. Libraries were diluted to 45 pM and pooled equally, with 9-10 individual samples per pool.