Illumina HiSeq 2500 paired end sequencing; Raw reads: thymus_evolution_TEC_AN118
Murine transgenic thymic epithelial cells expressing ancient transcription factors of the Foxn1/4 family were isolated by TEC digest and sorted. RNA was isolated according to standard phenol-chloroform RNA extraction protocols and libraries were prepared using Ultraâ„¢ RNA Library Prep Kit (Illumina).