RNAseq of Aricia agestis population from Swyncombe Downs (SWD)
Females were caught in the field and killed in the lab before being placed in RNAlater at 4C overnight. Samples were then kept in RNAlater at -80C until extraction. To extract total RNA, the thorax was dissected from each individual and homogenised. RNA was extracted using Qiagen RNeasy mini column protocol for RNA isolation from animal tissues. To remove genomic DNA, the extracted sample was then processed using the TURBO DNA-free kit. PolyA RNA isolation and pooled cDNA library preparation was then conducted by the Liverpool Genomics Center. Libraries were sequenced on three lanes of a 454 plate.