GSM5651283: spinal cord astrocytes, ALDH+, EA16; Mus musculus; RNA-Seq
Spinal cord was collected from LFNG-GFP  mice or ALDHL1L GFP C57BL/6J mice were dissected and dissociated, and astrocytes were isolated using FACS directly into QIAGEN RLT lysis buffer (Cat# 74004) with 1% ß-Mercaptoethanol. Bulk RNA was isolated using the RNeasy Micro Kit (74004, QIAGEN). Illumina sequencing libraries were constructed from 10ng of total RNA using the Trio RNA-Seq System (0507-96, NuGEN).