GSM5599268: S394_17_GFA_WT_524_Hip; Mus musculus; RNA-Seq
Adult microglia were harvested using density gradient separation. CD11b+CD45lo microglial cell populations were FACS-sorted and collected directly in cell lysis buffer. The sequencing run was performed on a HiSeq 1000 instrument and TruSeq SBS v3 Reagents according to the Illumina HiSeq 1000 System User Guide. Image analysis and base calling resulted in .bcl files, which were converted into .fastq files by the CASAVA1.8.2 software.