GSM5463745: G401_shCTRL_H3K4me1_1; Homo sapiens; ChIP-Seq
In brief, cells were cross-linked directly on the plate using 1% paraformaldehyde for 10 min at room temperature and quenched with glycine. Nuclei were isolated using the Covaris TruCHIP kit and Chromatin extracts were sonicated for 8 min using a Covaris E220 focused ultrasonicator at a peak power of 140, a duty factor of 5 and 200 cycles per burst. Chromatin immunoprecipitation was carried overnight with antibodies bound to dynabeads. Chromatin was de-crosslinked, digested with RNase and Proteinase K and DNA was purified using a Qiagen DNA kit. For MNase ChIP, CST kit guidelines were followed. KAPA HyperPrep Kit (Kapa Biosystems, Cat# 7962363001).