WGS of F. tularensis: isolate
A live culture of Francisella tularensis LVS strain NR-28537 was obtained from BEI Resources (Catalog No. NR-28537). Nucleic acid was extracted using Lucigens MasterPure Complete DNA and RNA Purification Kit (Catalog No. MC85200) and the resulting genomic DNA was quantified using the Qubit 4.0 Fluorometer, and quality checked on the TapeStation 2200 (DIN 6.9). Sequencing libraries were made using the ONT ligation library preparation kit (Catalog No. SQK-LSK109). The ONT library was sequenced with an R9 flowcell (Catalog No. FLO-MIN106D) on the ONT GridION platform.