GSM4730153: WT elongation rep1; Solanum lycopersicum; RNA-Seq
Tissue was ground in liquid N2 and RNA extracted with Plant RNA Reagent (Life Technologies; http://www.lifetechnologies.com). Total RNA was treated with 1X RNA Secure Reagent (Ambion; http://www.lifetechnologies.com) at 65C for 10 min, treated with DNase I (Ambion; http://www.lifetechnologies.com) at 37C for 10 min, and purified with RNeasy kit (Qiagen; http://www.qiagen.com) following the manufacturer's instructions. RNA-Seq library preparation and sequencing was performed at the Oregon State University Center for Genome Research and Biocomputing. Libraries were prepared using the TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego, CA) and sequenced as single-end 51 bpreads on two lanes of the Illumina HiSeq 2000.