GSM5911827: H9_TPRX2_OE48h_staccseq; Homo sapiens; OTHER
Stacc–seq was was performed as previously described with the wash version (Liu et al., 2020). Briefly, the collected cells were incubated with concanavalin-coated magnetic beads and antibody and pG–Tn5 mixture. Beads were washed twice and resuspended for Tn5 digestion, which was stopped by SDS. Then DNA was purified by SPRI beads for library amplification and next-generation sequencing. Stacc-seq libraries were prepared using Stacc-seq as previously described (Liu et al., Nature, 2020).