GBS sequence of Contarinia brassicola: whole body
Genomic DNA was extracted from individual canola flower midge specimens (whole body) using a QIAmp DNA Micro Kit. Samples labelled as 'noWGA'did not undergo additional whole genome amplification (WGA); DNA from all other specimens was amplified via WGA using a Qiagen REPLI-g WGA Mini Kit prior to library prep and sequencing. All samples underwent two-enzyme GBS library preparation following Poland et al. (2012), using the restriction enzymes PstI and MspI, and paired end sequencing was conducted on an Illumina Hiseq 2500.