GSM5699738: M2_IL4_4; Mus musculus; RNA-Seq
Polarized BMDMs were lysed with RLT Plus buffer with beta-mercaptoethanol, lysates were homogenized using a Qiashredder, and RNA samples were isolated according to the RNeasy Plus Mini Kit (Qiagen) protocol, which included gDNA eliminator spin columns. All RNA samples were tested for concentration and purity via NanoDrop (Thermo Scientific). RNA samples were stored at -80ºC. Libraries were constructed with the TruSeq mRNA kit (Illumina) using the manufacturer's protocol.