GSM6333454: Thp1-macro-H3K27ac-rep2; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a ultrasonicator (Bioruptor Pico). 4×10^6 cells and 3 μg antibody were used per immunoprecipitation. Antibody of H3K4me1 (Abcam, ab8895), H3K4me3 (Abcam, ab8580), H3K9me3 (Abcam, ab8898), H3K27ac (Abcam, ab4729), and H3K27me3 (Millipore, 07-449) were used in this study. The ChIP DNA was obtained using SimpleChIP® Enzymatic Chromatin IP Kit (CST, #9003). After immunoprecipitation, ChIP-seq library was constructed using NEBNext® Ultra™ DNA Library Prep Kit (NEB, E7370S).