GSM5856952: bulked RNAseq of root tips growning with nitrate replicate 2; Zea mays; RNA-Seq
primary roots were sampled and cut into small pieces of approximately 0.5mm and then digested in protoplasting solution (1.25% cellulase R10, 0.3% macerozyme R10, 400 mM mannitol, 20 mM 4-morpholineethanesulfonic acid, 20 mM KCl, 10 mM CaCl2 and 0.1% BSA). RNA library were perpared with the Chromium Single Cell 3' Reagent Kit v3 according to the manufacturer's protocol.