GSM5101204: 200ng_IP-10min; Homo sapiens; OTHER
Total RNA extraction, TRIzol reagent (Thermo Fisher Scientific, catalog no. 15596026) was used to cell suspension following the standard protocol. Cell lysate, the cells were resuspended in 3μL 0.2% (vol/vol) Triton X-100 (Sigma-Aldrich, cat. no. T9284) lysis buffer with 0.2 μL of Recombinant ribonuclease inhibitor. The lysate was incubated at 72 °C for 3 min and immediately put back on ice. Blood sample, red blood cells was removed by Red Blood Cell Lysis Buffer (Roche, cat. no. 11814389001). The total RNA was extracted using TRIzol reagent. For library preparation, all reactions were performed in one tube and followed the protocol of TruePrep® RNA Library Prep Kit for Illumina (Vazyme, cat. no. TR501-01) with some modifications. The m6A MeRIP experiments were performed following the recommendations from the EpiMark N6-Methyladenosine Enrichment Kit (NEB #E1610) with some modifications.