GSM5397189: H1_hESC_YAP_KO_UNT_1; Homo sapiens; RNA-Seq
RNA was extracted using Zymo columns and sent for library preparation and quality control. snATAC-Seq was carried out at the Center for Epigenomics at UCSD. The procedure followed the protocol described in Cusanovich et al. Briefly, DMSO-frozen hESCs were thawed in 37°C water bath until liquid (~2-3 minutes) and immediately placed on ice. Nuclei were isolated and permeabilized using OMNI buffer (0.1% NP40, 0.1% Tween-20, 0.01% Digitonin) as described40.  Nuclei were counted and 2k nuclei were dispensed per well; 96 wells per sample for tagmentation. After tagmentation (and addition of the first barcode), nuclei from individual samples were pooled. 20 nuclei from each sample were sorted per well (768 wells total). Following nuclear lysis the second barcode was added, and DNA molecules were pooled and purified. Before sequencing, the quality of the library was certified by analyzing fragment size distribution and DNA concentration Illumina TruSeq stranded library for RNA-Seq; combinatory barcoding protocol of Cusanovich et al. was used for snATAC-Seq (details see above extract protocol).