RNA-seq of sorted KIT+ cells from ACC PDX model ACCX22
RNA was extracted using the Nucleospin RNA XS Kit (Takara Bio 740902.50) according to manufacturer instructions. RNA quality was analyzed by Bioanalyzer (Agilent RNA Pico). Poly-A pull-down was used to enrich mRNAs from total RNA samples. Libraries were constructed using Illumina TruSeq stranded mRNA kit. RTA (Illumina) was used for base calling and bcl2fastq2 (version 2.20) was used for converting BCL to fast format, coupled with adaptor trimming