GSM4826599: P4-A3B; Komagataella phaffii; RNA-Seq
Cells were first digested in digestion buffer (50 mM EDTA, 500 mM sorbitol, pH 7.4) supplemented with 0.1% (v/v) 2-Mercaptoethanol and Zymolyase (Zymo Research, Irvine, CA) for 25 min at 30°C and 100 rpm shaking. Lysis and RNA purification was then performed using the RNeasy kit (Qiagen, Cat #74104). RNA quality was analyzed to ensure RNA Quality Number >7. KAPA HyperPrep kit (Roche, Basel, SUI)