GSM5897804: Pig_ileum_scRNA; Sus scrofa; RNA-Seq
Ileum crypts were isolated and digested with collagenⅠ and Tryple, alive cells were sorted by flow cytometry. Single cell suspensions were captured using the 10X Genomics Chromium Single cell 3' Solution according to the manufacturers protocol. Briefly, a single-cell suspension was mixed with RT-PCR master mix and loaded together with Single Cell 3' Gel beads and partitioning Oil into a Single Cell 3' Chip. About 5,000 cells were loaded into each reaction. RNA transcripts from single cell were uniquely barcoded and reverse-transcribed. ScRNA-seq libraries were generated using the Chromium Single Cell 3' Reagent Ki V3 (10X Genomics). The libraries were sequenced as paired-end with Illumina Novaseq 6000.