GSM5552438: 8x pooled E17 SCN, maternal SCN lesion, mother on time-restricted feeding regime killed at time 18; Rattus norvegicus; RNA-Seq
Fetal SCN samples collected at each of the 8 time points (time 0-21) were pooled from the same mother (n = 8 for Group A, n = 5-10 for Group B). RNA was isolated by RNeasy Micro Kit (Qiagen, Germany) according to manufacturer's instructions, treated with On-Column DNAse I digestion set (Sigma) for 15 minutes at 37°C, then quantified by spectrophotometry. RNA integrity was tested by capillary electrophoresis (Agilent Tech., USA). RNA libraries were prepared for sequencing by rRNA depletion, polyA RNA enrichment and using Smarter Stranded low input tot-RNA-Seq kit v2 (Takara, Japan). Illumina NextSeq 500/550 High output kit v2 (150 cycles, SE)