GSM5898239: Wounded TM Day 3; Mus musculus; RNA-Seq
TMs were removed and kept in cold PBS; The epithelial and fibrous layers were separated using dispase and separately digested using TryplE (epithelial) and Collagenase + DNAse (fibrous), filtered, and dead cells removed. Cells were loaded onto the Chromium Controller from 10x Genomcis for single cell capture. RNA libraries were prepared for sequencing using standard Illumina protocols