GSM5354107: HSC from normal liver (untreated) RSB014; Mus musculus; RNA-Seq
For single cell RNA-seq of the whole liver, hepatic cell populations were obtained by perfusing and digesting the liver and enriched in non-parenchymal. HSC were obtained perfusing and digesting the liver, followed by gradient centrifucation (Mederacke et al, Nat Protocol 2015) and FACS sorting for LratCre-induced TdTom. Single-cell RNA-seq libraries were prepared using the Chromium Single Cell 3′ v2 Reagent Kit (10x Genomics) according to manufacturer's instructions, 12 cycles of cDNA amplification and 12 cycles of library amplification were performed, and samples were sequenced on an Illumina HiSeq and NovaSeq 6000 sequencing system instrument at the JP Sulzberger Columbia Genome Center.