GSM5353460: Day1_10nM; Mus musculus; RNA-Seq
Total RNA was isolated using the RNeasy Mini Kit (Qiagen), according to the manufacturer's instructions. The quality of extracted RNA was evaluated using the RNA integrity number and Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The DNA libraries for mRNA-seq were constructed using the SureSelect Strand-Specific RNA Library Prep Kit (Agilent Technologies), according to the manufacturer's instructions.