GSM5911795: h_GV_18_Ribo_rep1; Homo sapiens; OTHER
Ribo-lite was performed as previously described (Xiong et al., NCB, accepted), after removing the zona pellucida, oocytes or embryos extact preparation by detergent lysis, RNase I digestion, ultracentrifugation and size selection through polyacrylamide gel, RPFs were subjected to a small RNA-seq library preparation method (CATS, Diagenode). Ribo-lite libraries were prepared using Ribo-lite developed in-house (accepted by NCB in principle), RPFs were subjected to a small RNA-seq library preparation method (CATS, Diagenode).