GSM5354248: MSC_16; Homo sapiens; RNA-Seq
The cryopreserved P1 BM-MSC from 17 donors were stabilized in RNAStill (Real Biotech Corporation) prior to thawing. Cells were thawed on ice and recovered by centrifugation. Total RNA was extracted with the Aurum total RNA mini kit (Biorad) including an on-column DNase I digestion step. RNA was quantified using a BioDrop (Montreal Biotech) and the integrity evaluated on an Agilent TapeStation; the RNA integrity numbers were in average 9.7 [±0.2]. Ribosomal RNAs were depleted and libraries were prepared with the TruSeq stranded total RNA kit (Illumina), then multiplexed and sequenced on an Illumina NovaSeq 6000 Sprime flow cell in a paired-end 50bp configuration.