GSM5652022: 1302_Normal; Homo sapiens; RNA-Seq
These are formalin-fixed paraffin embedded human tissues that underwent bulk RNA sequencing. Approximately 200-500 ng of FFPE RNA etracted from FFPE slides with a DV200 range between 3-99 or 65-100 ng of fresh frozen RNA per sample RNA library constructed using KAPA RNA Hyper library prep kit. Customized adapters with 3bp UMI and sample-specific dual-index primers were added. Approximately 500 ng of each RNA library were pooled for hybridization capture with IDT Whole Exome Panel V1 using a customized capture protocol modified from NimbleGen SeqCap Target Enrichment system. The captured libraries were sequenced on Illumina HiSeq4000 in paired ends (2x100bp) to a target 50 million read pairs per sample.