GSM5651836: IgG, DAXX WT cells; Homo sapiens; ChIP-Seq
Etoposide (10 uM) treated cells were harvested after 24 hours of treatment, fixed with formaldehyde for 10 min followed by quenching with glycine. 25 million cells per sample were sonicated using Covaris sonicator to obtain fragments of size between 100-500 bp. Chromatin was incubated with 10 mg of p53 antibody (Santa Cruz sc-126X) overnight followed by pull-down using protein- G Dynabeads (life technologies). Beads were washed, eluted and reverse cross-linked overnight. IP DNA was purified using Sigma PCR purification kit. Libraries were prepared using Illumina protocol and sequencing 75bp single end on Illumina Nextseq500 on high-output mode