GSM5419484: PLF_M1; Mus musculus; RNA-Seq
Total RNA from indicated cell types was isolated using RNeasy Micro kit (Qiagen). QuantSeq libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit (FWD) for Illumina together with the PCR Add-on Kit for Illumina and the UMI Second Strand Synthesis Module for QuantSeq FWD (Lexogen, 015.96, 020.96, 081.96), according to the manufacturer's instructions. Libraries for all cell types were prepared from 150 ng total RNA input and 15 cycles of library amplification were performed. Sample integrity was assessed using a Bioanalyzer 2100 (Agilent Technologies). Pooled libraries were 65 bp single-end sequenced on the HiSeq4000 (Illumina).