GSM5354313: An.stephensi infected with CSPwt rep2; Anopheles stephensi; RNA-Seq
Total RNA was extracted using the Trizol Reagent (Invitrogen, Carlsbad, USA) from a pool of 15 mosquitoes infected with CSPwt or CSPmut parasites. A total amount of 2 μg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using VAHTSTM mRNA-seq V2 Library Prep Kit for Illumina® following manufacturer's recommendations and index codes were added to attribute sequences to each sample.