GSM5857057: MuSCs_D0#1; Mus musculus; RNA-Seq
For MuSC samples, cells were isolated by enzymatic digestion of the indicated tissue and cells were purified by fluoresence activated cell sorting. 1000 cells were sorted in TCL buffer with 1% 2-mercaptoethanol and submitted for library construction and sequencing. For whole muscle and liver samples, tissue was homogenized in Trizol, and then RNA was isolated per the manufacterure protocol. 2ng of purified RNA were added to TCL buffer with 1% 2-mercaptoethanol and submitted for library construction and sequencing.