RNA-seq of Acraspis erinacei; adult female venom gland
To obtain the venom gland transcriptome, 15 venom glands were dissected from newly emerged adult females from wild-collected oak (oak spp.) hedgehog galls Acraspis erinacei and placed in RNAlater stabilization solution. Pooled tissues were homogenized in lysis buffer using a Bead Ruptor 12 (Omni International) with additional lysis with a 26-gauge syringe. RNA was extracted from the sample using the RNaqueous Micro kit followed by DNase I treatment as specified by the kit and confirmed to be of good quality using the Bioanalyzer 2100 (Agilent). The Illumina HiSeq library was prepared from 200 ng RNA using the TruSeq Stranded mRNA kit and sequenced in 150 bp single-end reads across two Rapid Run lanes on the Illumina HiSeq 2500 (Penn State Genomics Core Facility, University Park, USA) along with nine other barcoded wasp samples.