GSM5914648: WT_Si_22C_3; Arabidopsis thaliana; RNA-Seq
Total RNA was extracted using TRIzol® Reagent from the Si and CK samples grown at 22℃,25℃or 28℃. There were three biological replicates for each group. The raw paired end reads were trimmed and quality controlled by SeqPrep and Sickle with default parameters. Then clean reads were separately aligned to reference genome with orientation mode using HISAT2 software. The mapped reads of each sample were assembled by StringTie in a reference-based approach.