GSM5553307: wt_f; Mus musculus; RNA-Seq
Retinas were removed and the vitreous and other tissues were cleared with forceps. Retinas were digested with the Worthington Tissue Dissociation kit to yield single-cell preparations. Preps were then fixed with methanol and kept at -80ºC until sequencing. On day of sequencing, samples were rehydrated and loaded onto an individual lane of a 10X Genomics droplet sequencing chip each to generate cell-bead suspensions, using the Single Cell 3' v3 chemistry. Library construction was performed according to 10X Genomics standard protocols. Single Cell 3ʹ v3