GSM5553627: Liver tissue of MAFLD patient_rep2 [19054_D1]; Homo sapiens; RNA-Seq
Total RNA was extracted from liver tissue samples of patients with MAFLD and healthy controls using the Illustra RNA spin Mini Kit (GE Healthcare, USA). RNA samples were quantified using a Nano Drop instrument (Thermo Fisher Scientific, USA) and qualified by agarose gel electrophoresis. Illumina kits which include procedures of RNA fragmentation, random hexamer primed first-strand cDNA synthesis, dUTP-based second strand cDNA synthesis, end-repairing, A-tailing, adaptor ligation, and library PCR amplification, were used for RNA-seq library preparation.