GSM5352822: male cerebellum replicate1; Mus musculus; RNA-Seq
Total RNAs from mouse (C57BL/6J) adult brain, cerebellum, heart, colon, and gonad in both sexes were extracted. The RNAs were classified into two parts, one part was subsequently treated with Ribo-off rRNA Depletion Kit to remove ribosome RNA (rRNA). Then the VAHTS TM Stranded mRNA-seq Library Prep Kit for Illumina was used for strand-specific library preparation. After finished, the library was suquenced on the Illumina Nova platform. The another part was used to constructe for cDNA using P Clontech SMARTer PCR cDNA Synthesis Kit and PrimeSTAR GXL DNA Polymerase, followed by library preparation by using SMRTbellTM Template prep and sequencing on the Pacific Biosciences Sequel I platform.