GSM5912708: ATAC_Naive_rep1; Mus musculus; ATAC-seq
For snRNA-seq data, mouse trigeminal ganglia were dissociated into a single nuclei suspension as described in Renthal et al. 2020 and Yang et al. 2021. Briefly, tissue was dissociated by dounce homogenization and clarified by ultracentrifugation through a Optiprep gradient based on a protocol (Mo et al. Neuron 2015) and modified in Renthal et al. 2020. For snATAC-seq samples, TG were collected using a non-gradient protocol described previously (Slyper et al., 2020), and GFP+ nuclei were sorted and collected using FACS. inDrops libraries were constructued as described in Zilionis et al., Nat. Protoc. 2017. 10X Genomics gene expression assay and ATAC assay were prepared according to the user manuals.