GSM5206819: fls2_flg22_24h_2; Arabidopsis thaliana; RNA-Seq
Total RNAs were extracted using Sepazol-RNA I Super G (Nacalai tesque), followed by RQ1 RNase-Free DNase  (Promega) treatment. Libraries were prepared from 500 ng of total RNA. BrAD-seq (Townsley et al., 2015) was used to to create strand specific 3-prime digital gene expression.