GSM4824859: shRNA2_2; Homo sapiens; RNA-Seq
200,000 MDA-MB-468 cells infected with either pSicoR, shLMO2-1 or shLMO2-2 were harvested and RNA extraction was performed using the RNeasy Micro Kit (Qiagen #74034). mRNA was enriched using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (#E7490). cDNA libraries were constructed using the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (#E7770, #E7775). Libraries were fragment analyzed by LabChip and quantified by qPCR using the KAPA Library Quantification kit (#KR0405).