GSM4826922: C4 cns_lag3ko_cd45; Mus musculus; RNA-Seq
Single cell suspensions were resuspended in RPMI plus 2% FBS at a concentration of 1x106 cells/mL and barcoded with a 10x Chromium Controller (10x Genomics) according to the manufacturer's instructions. For each sample, we loaded 8.7µl with goal recovery of 5000 cells. RNA from the barcoded cells for each sample was subsequently reverse-transcribed and sequencing libraries were constructed with reagents from a Chromium Single Cell 3′ v2 reagent kit (10x Genomics) according to the manufacturer's instructions. Libraries were quantified and assessed for quality by Tape Station. Sequencing was performed with Illumina NextSeq according to the manufacturer's instructions (Illumina).