GSM5172052: GFPDay14-3; Mus musculus; RNA-Seq
Tibia were pulverized at liquid nitrogen temperature and homogenized using Bullet Blender Gold (Next Advance). Collection of Total RNA from homogenized tibia was performed using TRIzol extraction (ThermoFisherScientific) and RNeasy Mini Kits (Qiagen). The TruSeq Stranded Total RNA Library Prep Gold (Illumina) was utilized for next generation sequencing library preparation per the manufacture's instructions.