GSM6333529: SKMEL palbo 3; Mus musculus; RNA-Seq
Total RNA was extracted using Qiagen Rneasy Mini Kit (Cat #74106) according to manufacturer's instructions. RNA libraries were prepared for sequencing using the kits NEBNext® Poly(A) mRNA Magnetic Isolation Module (Cat# E7490) and NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (Cat#E7760), following manufacturer´s instructions and 9 cycles of library amplification