GSM6208917: PARK2-resPD rep1 line NP PDL 1.5L-wtParkin; Homo sapiens; RNA-Seq
Total RNA was isolated from neural progenitors using RNeasy Micro Kit (Qiagen, USA) followed by treatment with DNAse I (Qiagen, USA). The reaction was purifed with PureLink RNA Mini Kit (ThermoFisher, USA). RNA quality was checked by 2100 bioanalyzer (Agilent, USA). Enrichment of polyadenylated RNA and library preparation was performed with NEB Next Ultra II Directional RNA Library Prep (NEB, USA) according to the manufacturer's protocol. Samples were sequenced on NextSeq 500 System (Illumina, USA) with NextSeq 500/550 High Output Kit v2.5 (75 Cycles). Library preparation was performed with NEB Next Ultra II Directional RNA Library Prep (NEB, USA) according to the manufacturer's protocol.