GSM5961404: Replicate 1 Day1Adult Control always fed; Caenorhabditis elegans; RNA-Seq
Animals were collected at the respective stage as described above. At the time of collection, animals were washed off the plate with M9, washed 3x with M9, mechanically homogenized in TRIzolusing disposable tissue grinder  ).  Total RNA was isolated using RNeasy Minelute kit according to manufacturer (Qiagen). RNA-seq libraries were prepped using the Ovation Universal RNA-seq kit (Nugen) according to manufacters instructions