RNA-seq of Mucor irregularis : response to high temperature
Libraries were selected for cDNA target fragments of 200300 bp on 2% Low Range Ultra Agarose followed by PCR amplified using Phusion DNA polymerase (NEB) for 15 PCR cycles. After quantified by TBS380, paired-end libraries were sequenced by Illumina NovaSeq 6000 sequencing (150bp*7, Shanghai BIOZERON Co., Ltd).