GSM5625305: day 5 (day3+48hrs) RA treated  [R5-Rep1]; Mus musculus; RNA-Seq
At each timepoints, cells were washed with 1X PBS and then cell lysate was collected with RLT buffer (Qiagen). Total RNA was extracted using Rneasy mini kit (Qiagen) Libraries were constructed with Universal plus mRNA sequencing kit (NuGEN) and sequenced onto 2 lanes of NovaseqS1 to generate 30 million reads/sample.