RNA-Seq of mus musculus : adult female lung
Total RNA was extracted from infected mice lung using Trizol reagent . Ribosomal RNA was removed using EpicentreRibo-Zero rRNA Removal Kit .  the sequencing procedures were performed as follows. In brief purified RNAs were fragmented to approximately 200bp, and collected RNAs were subjected to cDNA synthesizing. The libraries were paired-end sequenced (PE150, 2150 bp) using IlluminaHiSeq 3000 platform. Clean data were aligned to the mouse reference genome (mm10) via software HISAT2 . HTSeq v0. 6.0 was used to count the reads numbers mapped to each gene. The whole samples expression levels were presented as TPM (Transcripts Per Kilobase of exon model per Million mapped reads), which is the most frequently reported RNA-seq gene expression values