GSM5667270: CD11b- BM "macrophage" - rep1; Mus musculus; RNA-Seq
Femoral bone marrow (BM) was collected by flushing the BM cavity with 2%FCS/PBS. Following staining with fluorochrome labelled antibodies, BM cell populations were sorted into 50% FCS/RPMI media, washed with 2%FCS/PBS and cell pellets snap frozen. RNA was harvested using Trizol reagent followed by QIAGEN RNeasy mini kit. RNA libraries were prepared for sequencing using SmartSeq2 (https://doi.org/10.1038/nprot.2014.006).