GSM4730245: Ldlr-/- mice on Western diet sample 2; Mus musculus; RNA-Seq
Liver (left lobe) was placed into RLT buffer for RNA extraction with RNeasy® Plus Mini Kit (Qiagen, #74136), The quality of purified RNA samples was determined using a Bioanalyzer 2100 (Agilent) with an RNA Eukaryote Total RNA Nano chip Samples have a minimum RIN of 9.0 and an average RIN of 9.53. Samples were prepared for sequencing using the NuGEN Ovation RNA-Seq system v2 reagents for cDNA preparation and the Ovation Ultralow DR Multiplex system for the adapter ligation step. Libraries were amplified by 9 cycles, according to the manufacturer's instructions.