GSM5050754: pLV; Homo sapiens; RNA-Seq
RNA was extracted using the RNeasy Mini kit (Qiagen). Polyadenylated mRNA was isolated from approximately 10 µg of total RNA using the Dynabeads mRNA purification kit (ThermoFisher Scientific) following the manufacturer's instructions. Nanopore libraries were prepared with 100 ng of poly(A)+ RNA using the Direct cDNA Sequencing kit (SQK-DCS109) with Native Barcoding (EXP-NB104) following the manufacturer's instructions (Oxford Nanopore Technologies). The final library was loaded onto an R9.4.1 MinION flow cell (FLO-MIN111, Oxford Nanopore Technologies) and sequenced for 72 hours. Oxford Nanopore Sequencing Direct cDNA Sequencing kit (SQK-DCS109)