GSM5912150: A4141_012; Homo sapiens; RNA-Seq
muscle biopsy portion was added in RNA-later containing 2ml Eppendorf tubes. RNA was extracted using automated extraction protocols on the QiaSymphony platform using the QiaSymphony RNA extraction kit (cat. 931636). RNA is quantified by Agilent Fragment Analyzer. Library preparation and was performed using the Strand-specific cDNA library. RNA samples were subjected to purification of poly-A containing mRNA molecules, fragmented mRNA fragmentation. Random primed cDNA synthesis (strand-specific) and adapter ligation and adapter specific PCR amplification was then performed.