GSM5354414: Splenic B cells_WT_1; Mus musculus; RNA-Seq
B cells were isolated from spleen by EasySepTM Mouse B cell Isolation Kit (STEMCELL Technologies, Vancouver, Canada). Thymocytes were isolated from thymus. Total RNA was extracted using AllPrep DNA/RNA Mini Kit (Qiagen, Hilden, Germany). Libraries were prepared from 100ng of total RNA using NEBNext UltraII Directional RNA Library Prep Kit for illumina (New England BioLabs, Ipswich, MA, USA) according to manufacture's protocol.