GSM5960578: 21683; Homo sapiens; Bisulfite-Seq
Genomic DNA was extracted from mononuclear cells using a Qiagen Mini or Midi Kit (Qiagen Gmbh). Quantitative and qualitative control of DNA was carried out by specific double-stranded fluorescence assay and gel migration. Preparation of RRBS libraries for 'paird-end' sequencing on Illumina® sequencer, with the Premium RRBS kit (Diagenode®), including an MspI enzymatic digestion step (targeting CCGG sites), size selection and bisulfite conversion. Paired-end sequencing of 2x100 bases on NovaSeq™6000 (Illumina®) was performed