GSM5310532: Cnot8-5-KO-24h_rep1; Mus musculus; RNA-Seq
Total RNA was extracted from cells using RNAzol Reagent. To avoid genomic DNA contamination, total RNA was treated with RQ1 RNase-Free DNase for 30 min at 37 °C, and subsequently purified using the phenol-chloroform extraction and ethanol precipitation. The mRNA enriched by Oligo(dT) was reversely transcribed, amplified by PCR, and utilized for the construction of Illumina sequencing library. RNA sequencing was performed using the Illumina Hiseq ×-Ten platform with 150 bp pair-end mode.