GSM5899091: C_19; Mus musculus; RNA-Seq
Mice were sacrificed, whole lungs prepared and immediately transferred to RNAlater solution (Qiagen). RNA was isolated using Qiagen Midi Kit according to the manufacturer's protocol. Quality and integrity of total RNA was determined using Agilent Technologies 2100 Bioanalyzer (Agilent Technologies; Waldbronn, Germany). A strand-specific RNA sequencing library was generated using NEBNext® Ultra  II Directional RNA Library Prep Kit (New England Biolabs) according to manufacturer's protocols. The library was sequenced on Illumina HiSeq 4000 using HiSeq 3000/4000 SBS Kit (300 cycles) with an average of 60M reads per RNA sample.