methylCap-Seq of Xenopus tropicalis : Tailfin (whole animal)
Purification of methylated DNA was carried out by affinity columns with methyl-DNA binding proteins (MethylCap Kit), following the manufacturer's protocol (Diagenode, Denville, NJ). Only the fractions corresponding to moderate to high levels of DNA methylation were kept. Libraries were prepared using NEXTflex ChIP-Seq Kit ( Bioo Scientific), using 30 ng of purified genomic DNA.