ITA-injected Apoe mice CD45 cells from tandem stenosis scRNAseq
To isolate cells, tandem stenosis segments were incubated (37oC, 1 h) in RPMI containing 1.7 mg/mL liberase (Roche), 1mg/mL hyaluronidase and 10U/mL DNAse. Single-cell suspension was stained with Live/Dead and anti-CD45 reagents and live CD45+ cells were FACS-separated. The cells were processed using standard Chromium 10x Single Cell 3' v3 chemistry and Illumina HiSeq 3000 was used for deep sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols