GSM5353739: Wild-type memory B cell replicate 2; Mus musculus; RNA-Seq
Splenocytes were isolated then stained with antibodies required for FACS. RNA from sorted cells was obtained using QIAseq FX Single Cell RNA Library Kit. QIAseq FX Single Cell RNA Library Kit (QIAGEN) was used to extract RNA from ~1000 sorted NP-specific WT and DKO memory B cells and prepare cDNA libraries enriched for polyadenylated RNAs, according to manufacturer's instructions. Library cleanup was performed using GeneRead Size Selection Kit (QIAGEN).