DNA-Seq of L. maculans: isolated phytopathogen-carrying canola seeds
the extracted DNA (~1ug) were fragmented using Celero Enzymatic Fragmentation, then followed by adaptor ligation and beads purification within two hours, according to the protocol of ExCell Bio library construction kit (NuGEN, San Carlos, CA, USA). After quality-control using an Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA, USA), libraries were sequenced on an Illumina HiSeq 2500 instrument using 150 base-length read chemistry in paired-end mode.