GSM6248691: shHDAC3 rat anti-HDAC3 second replicate; Drosophila melanogaster; OTHER
Crude nuclear extract was prepared in Nuclear Extraction buffer (20 mM HEPES pH 7.9, 10 mM KCl, 0.5 mM spermidine, 0,1% Triton X-100, 20% glycerol) followed by binding to Concanavalin A-coated beads (Polysciences, Inc. 86057-3) and incubation with primary antibody. Tagmentation was performed using Protein A-Tn5 fusion protein. Following tagmentation DNA was amplified using primers from Nextera Index Kit.