GSM5857206: MΦ Dusp6 KO_1; Rattus norvegicus; RNA-Seq
Single-cell suspensions of infarcted cardiac tissues were obtained by digestion with Liberase DL (Roche, 5401160001). Macrophages were stained with fluorescence-labeled antibodies against cell surface markers: anti-rat CD45 (OX-1)-PE-Cy7 (BD Biosciences, 561588) and anti-rat macrophage (HIS36)-PE (eBioscience, 12-0660-82) and selected by flow cytometry (MoFlo XDP). Selected macrophages were harvested directly into Trizol reagent. Total amounts and integrity of RNA samples were assessed using RNA Nano 6000 Assay Kit of Bioanalyzer 2100 system (Agilent Technologies, CA, USA). RNA libraries were prepared using KAPA RNA HyperPrep Kit (8098140702, Roche).