GSM4825436: Glucose_rep_B1; Xanthomonas citri pv. citri; RNA-Seq
RNA was harvested from X. citri cultures at mid-exponential phase using the Trizol/chloroform protocol.  Genomic DNA was then removed, followed by purification of the samples with the RNeasy Mini Kit (Qiagen). Then, 2-2.5 µg of RNA was used for depletion of rRNA using the Ribo-Zero rRNA Removal Kit (Gram-Negative Bacteria - Epicenter Biotechnologies). The preparation of the cDNA libraries was achieved with the Illumina TruSeq Stranded Total RNA kit according to the manufacturer's protocol.  Libraries were sequenced on the Illumina HiSeq 2500.