GSM5553476: 8930: wt CM D90 3; Homo sapiens; RNA-Seq
RNeasy kit (Qiagen) 0.1-1μg of RNA was used as input for a modified Illumina TruSeq Stranded mRNA protocol.Reverse transcription was performed with RNaseOut (Invitrogen) and actinomycin-D (MP Biomedicals) and the product was cleaned using AMPure RNAClean beads (Beckman Coulter). Second-strand synthesis was performed substituting dUTP for dTTP. After size selection, UTP containing strands were cleaved using USER (New England Biolabs)