GSM5584013: MoMac_GFP high 2; Mus musculus; RNA-Seq
Lungs were perfused and processed into a since cell suspension which was immunostained with a cocktail of fluorescent antibodies. Monocyte derived macrophages were FACS sorted directly into Trizol. RNA was extracted using the Rneasy Micro Kits (Qiagen) according to manufacturer's instructions. RNA was quantified and quality controlled using Qbit and a Bioanalyzer. 0.5–1 ng of RNA was retrotranscribed and processed to cDNA libraries using the Smart-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Bio). All samples were pooled together and loaded onto one lane. Libraries were sequenced on an Illumina NextSeq 550 system.