Single cell RNA-seq of ACC PDX model ACCX22
After sorting, cells were resuspended in PBS with 10% FBS at a concentration of 1000 cells/µl. 5000 cells per sample were targeted for sequencing. scRNA seq libraries were prepared using the 10x Genomics Chromium Single Cell 3' Solution with the Single Cell 3' v3 chemistry. Samples were mapped to human transcriptome GRCh38-3.0.0 and data was analyzed with cellranger pipeline version 3.1.0