GSM5784470: sev_2; Homo sapiens; RNA-Seq
Total RNA was isolated using TRIzol® reagent. Strand-specific adapters were added to fragmented RNA (average fragment length 200 nt) before reverse transcription followed the manufacturer's instructions. The quality of cDNA libraries was quality evaluated on an Agilent 2100 Bioanalyzer and sequenced by Shanghai Genergy Co. Ltd.