GSM5699722: Carm1_wildtype, biological rep4; Mus musculus; RNA-Seq
Trizol extraction of total RNA was performed for each sample from flash frozen muscle. Total RNA samples were quantified using Qubit HS RNA assay (Thermo) and fragment size was evaluated using the Fragment Analyzer HS NGS assay (AATI)Library construction was performed using Truseq RNA v2 (Illumina). RNA libraries were prepared for sequencing using standard Illumina protocols