GSM5850387: ESC-HA_K27L_24_rep2-ChIP; Mus musculus; ChIP-Seq
Chromatin was digested using MNase. Barcoded adapters that contain a T7 promoter were ligated to the DNA ends of the fragmented nucleosomes, and samples were pooled and splitted according to the different antibodies. We used T7 promoter to amplify the ChIP DNA in an in vitro transcription reaction. A second barcode was added during the library construction - as is described in van Galen et al. (2016).