GSM5824057: C4, hBMSCs, exosomes; Homo sapiens; miRNA-Seq
Total RNAs were extracted from exosomes using Qiagen RNEasy Mini- Plus kits (Cat. No. 74134). RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA). A total amount of 3 μg total RNA per sample was used as input material for the small RNA sequencing libraries were generated using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB, USA.). The library quality was assessed on the Agilent Bioanalyzer 2100 system using DNA High Sensitivity Chips. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq SR Cluster Kit v3-cBot-HS (Illumia).