GSM5699127: RHT6128: B1_cLVX-; Homo sapiens; RNA-Seq
iHeps cultured in mono-culture and co-culture were separated by magnetic activated cell sorting: iKC bind to CD163 beads, leaving iHep in the flowthrough. Cells were snap frozen in liquid nitrogen before storage in -80C until RNA extraction by TRIzol. Illumina Stranded mRNA multiplexing was done with unique 96 dual indices, and all samples sequenced on the same lane of NovaSeq 6000 S4 flowcell.