GSM5857202: IARS1_RFP-rep2; Homo sapiens; RNA-Seq
Total RNA was isolated using Trizol LS reagent (Invitrogen) and stored at –80°C until further processing. mRNA was isolated using Poly(A) Beads (NEXTflex). Sequencing libraries were prepared using the Rapid Directional RNA-Seq Kit (NEXTflex) and sequenced on a NextSeq500 (Illumina) to produce 75 base long reads (Utrecht DNA Sequencing Facility).