GSM5914539: fetal pancreas 12PCW_section 1; Homo sapiens; RNA-Seq
Spatial transcriptomics was carried out using the 10x Genomics Visium platform. 10μm tissue sections from OCT-embedded fresh frozen human pancreases at 12, 15, 18 and 20 PCW were mounted onto Visium Spatial slides and the sections were permeabilised for 30min to release mRNAs, which bind to the spatially barcoded-oligos present in the underlying spots and reverse transcribed, according to the manufacturer's protocol. Libraries were prepared from cDNAs according to the 10x Visium protocol and cDNAs were sequenced on the Illumina NextSeq 2000 platform