GSM5698767: C2H4_2_1; Cucumis sativus; RNA-Seq
After cutting the primary roots, every ten explants with the same size were selected and placed in Petri dishes containing 70 mL of with distilled water or 0.5 μM ethrel, and the solutions were changed every 24 h. At 48 h after treatment, samples (cucumber explants) were collected and stored at −80 °C until being used.Total RNA for RNA-Seq and qRT-PCR analyses was extracted from about 500 mg cucumber explants using Trizol reagent (Sangon, Shanghai, China) according to the manufacturer's instructions. Total RNA was dissolved in 100 ml of RNase-free water and was quantified using a NanoDrop spectrophotometer (Thermo Scientific). RNA quality was evaluated using the 6000 Pico LabChip of the Agilent 2100 Bioanalyzer (Agilent, Shanghai, China). RNA samples were sent to Novogene Co.Ltd (Beijing, China) for cDNA library construction and sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols