GSM5100593: Control1_IP; Sus scrofa; RIP-Seq
Using 90% DMEM/HIGH GLUCOSE (HyClone, Logan, Utah, USA) and 10% fetal bovine serum (FBS) (HyClone), penicillin (100 U/mL), and 1% streptomycin (100 μg/mL) at an atmosphere of 95% O2, and 37℃under 5% CO2. Medium was changed every 2d and cells were passaged by trypsinization at 80-90% confluency Total RNA was isolated using TRIzol (Invitrogen) reagent Extract total RNA, establish IP library and input library respectively, and use Illumina Novaseq™ 6000 for paired-end sequencing