GSM5419312: Intestinal crypts, KO_4; Mus musculus; RNA-Seq
ntestinal crypt cells were isolated from the intestines of four KO mice and four littermate controls by incubation with 10 mM EDTA, 10 mM HEPES and 2% FBS in HBSS for 15 min at 37°C. The crypt fractions were collected by vigorous shaking and filtered through a 70 μm cell strainer. The gathered crypt cells were centrifuged at 1200 rpm for 5 min. Total RNA was isolated from the collected crypt cells using TRIzol reagent. RNA libraries were prepared for sequencing using standard Illumina protocols