GSM5353478: KV22924; Mus musculus; RNA-Seq
Organoids were then washed twice in 1X DPBS for immediate RNA isolation using the Arcturus PicoPure RNA Isolation Kit. RNA was subjected to sample quality control by the Genomics and RNA Profiling Core of Baylor College of Medicine using the Nanodrop ND-1000 and a Bioanalyzer Nano Chip. RNA samples representing biological replicates from shLUC and shRor2 groups were then subjected to library preparation and Illumina Next Generation Sequencing on a NovaSeq 6000 (100 bp Paired-End Sequencing, 68 million reads per sample).