GSM5311382: HEL-Y149F-G19-1; Homo sapiens; RNA-Seq
RNA was extracted using Direct-zol RNA Microprep (ZYMO RESEARCH, R2061) and cDNA synthesized by qScript cDNA Super-Mix (Quanta bio, #95048-100), according to the manufacturer's instructions. RNA-seq library prep was carried out using the Illumina TruSeq Total Total Stranded kit (RS-122-2201) with Ribo-Zero rRNA reduction following the manufacturer's protocol without modification.