GSM5419641: N2 + vha-16 RNAi_rep2 [E2]; Caenorhabditis elegans; RNA-Seq
On the day of the extraction, 1 ml of TriPure Isolation Reagent (Cat. 11667165001, Roche) was added to each tube. The samples were then frozen and thawed quickly 8 times in liquid nitrogen to rupture cell membranes. RNA was then extracted by using a column-based kit from Macherey-Nagel (Cat. 740955.250). RNA libraries were prepared for sequencing using standard BGISeq500 protocol with polyA enrichment