GSM5419471: NCC-IT-A3 Barasetib_1; Homo sapiens; RNA-Seq
Culture supernatant were removed and cells were washed by PBS twice. Then, the total RNAs were extracted by using Rneasy mini kit (Qiagen, #74106). TruSeq Standard mRNA LT Sample Prep Kit  was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using TruSeq Stranded mRNA Sample Preparation Guide, Part # 15031047 Rev. E