GSM5206812: stp1/4/13_flg22_24h_1; Arabidopsis thaliana; RNA-Seq
Total RNAs were extracted using Sepazol-RNA I Super G (Nacalai tesque), followed by RQ1 RNase-Free DNase  (Promega) treatment. Libraries were prepared from 500 ng of total RNA. BrAD-seq (Townsley et al., 2015) was used to to create strand specific 3-prime digital gene expression.