chloroplast genome sequence of Camellia semiserrata: Mature plant
To generate chloroplast assemblies, a 270- or 350-bp insertion library was constructed for each species, using TruSeq DNA sample preparation kits, and sequenced using Illumina technology with 150-bp paired-end sequencing. A total of 72 million raw reads were generated and made available in FASTQ format. The quality of the raw sequence reads was evaluated using the software package FastQC. The software Trimmomatic v0.36 was used for removal of adapter, contaminant, low-quality (Phred scores <30), and short (<36 bp) sequencing reads. The remaining high-quality sequencing reads were assembled de novo using the NOVOPlasty pipeline v2.7.2 with default parameters and based on a kmer size of 39 or 23 following the developer's suggestions, where the psbA gene of C. oleifera was used as a seed input.