Pernospora effusa isolate A-03 genomic sequencing reads
P. effusa spores were harvested from non-wilting leaves that contained sporulating from spinach plants and brought into suspension in tap-water. Soil and other large contaminants were removed by filtering the spore suspension over a 50m nylon mesh filter followed by a filtering over a 11 m nylon mesh filter using the Merck All-Glass Filter Holder (47mm) and a vacuum pump. Remaining spores were resuspended in 100 mL of autoclaved tap water and filtered again. This washing step was repeated 10 times. P. effusa spores were freeze-dried and lysed using glass beads in a tissue lyser (2 x 30 seconds on 30 Hz). DNA was extracted from the pulverized spores with the Qiagen DNeasy Plant Kit using the and quantified with the Qubit 2.0 fluorometer using the Qubit dsDNA HS (High Sensitivity) assay kit. The library was sequenced on an Illumina Nextseq machine in high output mode with a 550 bp genomic insert paired-end 150 bp reads.