RNA-Seq of homo sapiens: Substantia nigra pars compacta: Sample pPDsHSrSNxi4568d200429PosA
"Nuclei suspensions from human SN/caudate nucleus were generated from fresh frozen tissues provided as whole midbrain or caudate nuclei. Dissociation and nuclei isolation was performed using a previously published protocol: https://www.protocols.io/view/frozen-tissue-nuclei-extraction-for-10xv3-snseq-bi62khge. Flow sorting parameters for DAPI gating are described in Martin et al (https://www.protocols.io/view/frozen-tissue-nuclei-extraction-for-10xv3-snseq-bi62khge). For the NR4A2 positive selection on a flow sorter, a DAPI vs 647 gating was established by selecting the 2.5%-4% highest fluorescent NR4A2 nuclei. Nuclei were loaded into the 10x Chromium Single Cell 3' V3 system. Reverse transcription and library generation were performed according to the manufacturer's protocol."