GSM5464615: MSG11; Homo sapiens; RNA-Seq
Labial MSG biopsies were obtained from healthy volunteers . MSG tissue biopsies from four healthy volunteers were mechanically dissociated using the m_lung_01.01 program on the gentleMACS dissociator (Miltenyi Biotec). Tissues were then enzymatically digested with 610 U/mL Collagenase type 2 (Worthington) and 0.04 mg/mL DNase I (Sigma) for 40 minutes at 37oC with agitation. Cells were further dissociated using m_lung_02.01 program on the gentleMACS dissociator. The digested tissue was filtered through a 70 μm cell strainer (Miltenyi Biotec), washed and resuspended in RPMI 1640 (supplemented with 2% FBS, 50U/mL penicillin and 50 μg /mL streptomycin). Single-cell suspensions were loaded onto a 10X Chromium Controller (10X Genomics) and library preparation was performed according to the manufacturer's instructions for the 10X Chromium Next GEM Single Cell Library kit v3 (10X Genomics).  Libraries were then pooled and sequenced on 4 lanes on a NextSeq500 sequencer (Illumina) using 10X Genomics recommended reads configuration.