GSM5268966: MCF10A replicate1-24h DMSO; Homo sapiens; RNA-Seq
RNA was extracted using RNeasy Mini Kit (QIAGEN) according to manufacturer instructions. RNA quality control was performed using an Agilent Bioanalyzer 2100. Sequencing library preparation was performed at the Stanford Functional Genomics Facility using the KAPA Stranded mRNA-Seq Kit, with KAPA mRNA Capture Beads (Roche) according to manufacturer instructions. Single-index 6-mer adapters were added, and the libraries were sequenced with 75 bp single-end reads on an Illumina NextSeq platform (Stanford Functional Genomics Facility).