GSM5464712: VIC Control rep2; Mus musculus; RNA-Seq
After trypsinization, RNA was extracted using an RNeasy micro kit following the manufacturer's recommended procedure. A total of 50 ng of total RNA from VICs was used to construct cDNA libraries. Sequencing libraries were constructed using amplified cDNA with the TruSeq RNA Sample Preparation kit v2 (Cat No. RS-122-2001 & RS-122-2002, Illumina Technologies., USA) for the HiSeq2500 system (Illumina) and sequenced using the 100-bp paired-end mode of the HiSeq Rapid SBS Kit v2 (200 Cycle, Cat No. FC-402-4021) and the HiSeq® Rapid PE Cluster Kit v2 (Cat No. PE-402-4002) with an Illumina HiSeq 2500 system (Illumina Technologies, USA).