GSM5552660: Patient Derived Xenograft model BCM-4195; Homo sapiens; Mus musculus; RNA-Seq
PDX tumor were harvested from mice and trimmed of excess fat pad tissue. Representative tumor fragments were snap frozen in liquid nitrogen. Tumor was homogenized and RNA extracted using the RNeasy Mini Kit from Qiagen. Total RNA was extracted from PDX samples and 10ng was used to generate and amplify whole transcriptome cDNA (NuGen Ovation v2, NuGen Technologies). Illumina TruSeq DNA-Seq library was quantified with the Kapa qPCR Library Quantitation Kit (Kapa)