GSM5206844: HeLa control DMSO - H3K4me2 -replicate 1; Homo sapiens; ChIP-Seq
Nuclear chromatin extracts were prepared from 1% formaldehyde crosslinked HeLa cells and Histone-DNA complexes were immunoprecipitated with H3K4me2 antibody. DNA was purified by phenol-chloroform extraction and ethanol precipitation. Libraries were prepared using the NEBNext Ultra DNA Library Prep for Illumina kit (#E7370) according to the manufacturer's protocol.