GSM5823986: Sample_5_Ribo-Seq S51A_veh_1; Mus musculus; OTHER
Cells were quickly washed twice in ice-cold PBS, and harvested in 400uL polysome lysis buffer by scraping on ice Ribo-seq libraries were constructed as previously described (McGlincy, 2017). rRNA was depleted using RiboCop following manufacturer's instructions (Lexogen). Total RNA libraries were built with Illumina TrySeq kit using rRNA depletion. Novaseq S1 1x100bp