GSM4826872: RNA.P4 rep3; Komagataella phaffii; RNA-Seq
KAPA HyperPrep kit (Roche, Basel, SUI) Cell samples from cultures expressing P[4] or P[6] were transferred to a shake-flask containing 500 mL of pre-warmed fresh BMMY media to achieve an OD600 = 0.5. Cell harvest, cell lysis, and recovery of ribosome footprints were performed as described previously (Taggart et al., Cell Syst. 2018). Samples were split following lysis for total RNA-Seq and ribosome profiling (Ribo-Seq).