ZmPep3 2-hour treated B73 leaf 5_1
Total RNA was isolated with the NucleoSpin RNA plant kit (Clontech), treated with the Turbo DNA-free kit (Ambion) and quantified using Qubit 2.0 fluorometer (Life Technologies). Preparation of RNA-seq data was done by Novogen Corporation. Briefly, insert size was checked on an Agilent 2100 Bioanalyzer (Agilent Technologies), and sequencing of the 250300-base-pair (bp) insert cDNA library was conducted using Illumina HiSeq platform PE150.