GSM5898047: BCM3392Vs017; Homo sapiens; RNA-Seq
Fresh tumors were preserved in RNAlater and frozen at -200°C. Copurification of miRNA and total RNA was performed using a miRNeasy Mini Kit (QIAGEN, 217004). The RNA concentration and purity were evaluated with an ND-1000 spectrophotometer (NanoDrop, Thermo Fisher Scientific, Wilmington, DE, USA). Quality metrics were the RNA Integrity Number (RIN) and DV200 value (% of RNA fragments with a length ≥200nt). 100 ng total RNA per sample was used as input material for the library preparation. 100 ng total RNA per sample was used as input material for the library preparation. The libraries were prepared with the KAPA RNA HyperPrep Kit (Kapa Biosystems, KK8541) and KAPA Target Enrichment using the Kapa HyperCap V3.0 kit for Illumina following the manufacturer's recommendations. Second-strand sequencing of the libraries was performed using the NuGEN (San Carlos, CA, USA) RNA-Seq System