GSM5584826: Muscle-DQ-1; Mus musculus; RNA-Seq
Quadriceps muscle were collected in Trizol solution (Invitrogen, Carlsbad, CA, USA) and RNA isolation was performed following the instructions. cDNA libraries were prepared according to the manufacturer's instructions for the TruSeq Stranded mRNA Sample Prep Kit (Illumina, San Diego, CA). The concentration and size distribution of the completed libraries were determined using an Agilent Bioanalyzer DNA 1000 chip (Santa Clara, CA) and Qubit fluorometry (Invitrogen, Carlsbad, CA).