GSM5311354: DRG cells - spared nerve injury (SNI), day 7; Mus musculus; RNA-Seq
Mice were anesthetized and perfused prior to dissection of dorsal root ganglions (DRGs, L3 + L4 from both sides). Whole DRGs were subsequently enzymatically (collagenase, dispase) and mechanically dissociated to a single cell suspension and passed through a 40-micron cell strainer. The 3' expression library was generated with Chromium Single Cell 3' Library, Gel Dead & multiplex kit and Chip Kit (v3, 10x Genomics).