GSM5857102: MEHP + ATRA_412-436; Mus musculus; RNA-Seq
Total RNA was extracted using Trizol (ThermoFisher Scientific) and purified from the aqueous phase of the extract using the Arcturus PicoPure kit (ThermoFisher). The RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer's instructions (New England Biolabs, Ipswich, MA, USA). Briefly, mRNAs were initially enriched with Oligod(T) beads. Enriched mRNAs were fragmented for 15 minutes at 94 °C. First strand and second strand cDNA were subsequently synthesized. cDNA fragments were end repaired and adenylated at 3'ends, and universal adapters were ligated to cDNA fragments, followed by index addition and library enrichment by PCR with limited cycles.