GSM5699376: s01B, scrambled, batch 4; Mus musculus; RNA-Seq
Following treatment, RNA from primary cortical cells (DIV5) was extracted and purified using the RNeasy Mini Kit 50 (Qiagen), according to the manufacturer instructions for cells grown in a monolayer. All RNA sample amounts were measured with a NanoDrop ND-1000 spectrophotometer (ThermoFisher Scientific). RNA quality was assessed on a Fragment Analyzer (Advanced Analytical Technologies) and all RNAs had a RQN between 8.8 and 9.5. RNA-seq libraries were prepared using 1000 ng of total RNA and the Illumina TruSeq Stranded mRNA reagents (Illumina). Cluster generation was performed with the resulting libraries using the Illumina TruSeq SR Cluster Kit v4 reagents and sequenced on the Illumina HiSeq 2500 using TruSeq SBS Kit v4 reagents.