GSM5419289: SCAB1-1; Arabidopsis thaliana; OTHER
Plasmids were extracted according to the manual of TIANprep yeast plasmid kit (TIANGEN, China). The prey inserts were amplified by PCR. The PCR products were sheared into 200-300 bp DNA fragments by Bioruptor Plus (Diagenode). End repair and adaptor ligation were performed by using NEBNext Ultra II End Repair/dA-Tailing Module and NEBNext Ultra II Ligation Module (NEB). The sequencing libraries were constructed by using PCR.