GSM5311377: PBMC 1; Homo sapiens; RNA-Seq
RBC lysis, stain with antibodies to CD45, CD34, CD73, CD90, CD105, CD235 and DAPI.  FACS sorting sends DAPI+ and CD235+ to Waste , Lin+ fraction contains cells positive for all other antibodies, while Lineage negative fraction would contain cells not tagged by these antibodies.  Total RNA was extracted from fractions using microRNA kit (Qiagen #74004) Ribosomal RNA was removed using the RiboGone Kit, followed by cDNA synthesis using the SMARTer Universal Low Input RNA Kit for Sequencing (Takara Bio). Libraries were prepared using the Ion Plus Fragment Library Kit