Amplicon Deep sequencing of several sgRNAs treated by CRISPR/Cas9: foxtail millet
For deep amplicon sequencing, two rounds of PCR were performed. In the first round PCR, PCR products containing the target site were amplified using gene-specific primers. In the second round PCR, primer sets with forward and reverse barcodes were used and limited the products size within 200 bp for library construction and Illumina sequencing.