GSM5897865: LM shWDR5 Replicate 3; Homo sapiens; RNA-Seq
Cells from knockdown control or shWDR5 group were harvested with QIAzol Lysis Reagent (Qiagen) and homogenized using QIAshredder tubes (Qiagen). For each cell line, shRNA expression was induced with doxycycline (1 ug/mL) for 3 days and 3 biological replicates were harvested at different passages. RNA isolation was performed using miRNeasy with on-column DNase digestion. ERCC spike-in RNA was added in proportion to the number of cells obtained during cell counts. Library generation was performed using TruSeq stranded mRNA library prep kit (Illumina). Paired-end sequencing was performed using an Illumina HiSeq4000 sequencer, generating an average of 59 million reads per library. TruSeq stranded RNA-seq