GSM5396987: DAA-12; Homo sapiens; RNA-Seq
Cells resuspended in the reaction mix were loaded into the Chromium Single Cell Chip B, as well as the 10x Barcoded Gel Beads and the partitioning oil. The Chip B was placed into the 10x Chromium controller for cell partitioning, targeting a final number of 10 thousand cells per sample. After partition was concluded, the GEMs (Gel Bead-In EMulsions) were transferred to the C1000 Touch Thermal Cycler for the first phase of reverse transcription and samples proceed to library preparation following manufacturer's instructions.