GSM6280540: HEK293 cells Ribo-seq [SM090F]; Homo sapiens; OTHER
Lysates were clarified, treated with RNase I, and subjected to sucrose cushion to isolate ribosome footprints. RNA was then extracted using Trizol and footprints of the specified size were retained by gel purification. RNAs were ligated to a universal DNA linker followed by reverse transcription. cDNAs were then circularized and PCR amplified. Qiagen Fast-Select kit is used for removing rRNA during reverse transcription.