GSM5464744: CDX2 -/-_ATAC-seq; Homo sapiens; ATAC-seq
The precipitation were lysed within 50 µL lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.2% (v/v) IGEPAL CA-630) and centrifuged at 500 g for 5 min at 4℃, then fragmentated with 50µL transposition reaction mix of TruePrep DNA Library Prep Kit V2 for Illumina® (TD501, Vazyme) under an 37℃ incubation condition for 30min, purified using a MinElute Kit (QIAGEN). The fragmented genome suspension was amplificated 8-13 cycles with 50µL amplification mix of TruePrep DNA Library Prep Kit V2 for Illumina® (TD501, Vazyme), and purified using a MinElute Kit (QIAGEN).amplification mix of TruePrep DNA Library Prep Kit V2 for Illumina® (TD501, Vazyme), and purified using a MinElute Kit (QIAGEN).