GSM5553007: MB46_III; Xenopus laevis; RNA-Seq
Brain regions were collected from the appropriate stage and total RNA was extracted using the mirVana kit (Life Technologies, USA). DNase treatment was used to remove genomic DNA and was followed by clean-up using RNeasy mini kit (Qiagen, USA). 1µg of cDNA was sheared in microTube (Covaris) and then used for library preparation (KAPA Taq PCR kits). The next generation sequencing was done using HiSeq2000 platform (Illumina; RRID:SCR_020132)