GSM5464088: SKNAS_C1_ATACseq; Homo sapiens; ATAC-seq
Briefly, 50,000 cells per replicate were pelleted and then resuspended and lysed in 50 uL ATAC resuspension buffer supplemented with 0.1% NP40, 0.1% Tween-20, and 0.01% Digitonin. After lysis, nuclei were pelleted and transposed using 2.5 uL Tn5 transposase in a 50 uL reaction. Finally, the transposed DNA was purified using a commercial PCR cleanup kit and libraries were amplified and prepared for sequencing. Paired end sequencing (2x75) was performed on an Illumina NovaSeq.