RNAseq of S. Lycopersicum apicales leaves
1)mRNA enrichment and purification: Oligo dT Selection to enrich the mRNA or rRNA depletion (For total RNA extracted from whole bloodglobin mRNA are depleted ) 2)RNA fragment and reverse transcription 2)RNA fragment and reverse transcription (For stranded specific mRNA librariessecond-strand cDNA synthesis with dUTP instead of dTTP ) 3)End repair, add A and adaptor ligation 4)PCR 5)Single strand separation and cyclization 6)DNA nanoball synthesis 7)Sequencing on DNBseq platform