WGS of F. tularensis: isolate
A live culture of Francisella tularensis LVS strain NR-28537 was obtained from BEI Resources (Catalog No. NR-28537). Nucleic acid was extracted using Lucigens MasterPure Complete DNA and RNA Purification Kit (Catalog No. MC85200) and the resulting genomic DNA was quantified using the Qubit 4.0 Fluorometer, and quality checked on the TapeStation 2200 (DIN 6.9). Sequencing libraries were made using Illuminas Nextera XT library preparation kit (Catalog No. FC-131-1024). The Illumina library was sequenced on a MiSeq sequencer using a 600 flow-cycle reagent kit (Catalog No. MS-102-3003) to produce 2x300 paired end reads.