Obovaria_unicolor
DNA was extracted using pellet pestles to grind the gut tissue in the extraction buffer from the DNeasy PowerSoil DNA Isolation Kit (Qiagen, Germantown, MD). The homogenate was briefly vortexed, centrifuged (2,000g,1 min) and ground again before direct use in the standard extraction protocol.Dual-indexed barcoded primers were used to amplify theV4 region of the 16S rRNA gene of theextracted DNA following established techniques (Kozich, Westcott, Baxter, Highlander, & Schloss, 2013). The amplified 16S rRNA gene fragments were combined and spiked with 20% PhiX before being sequenced on an Illumina MiSeq at the University of Mississippi Medical Centre Molecular and Genomics Core Facility.