RNAseq of Lophelia pertusa (genotype 142) exposed to T-8C, pH-7.9, dispersant
Total RNA was extracted from the coral fragments following a modified Trizol/Qiagen RNeasy Kit extraction protocol (Polato et al. 2011; Burge et al. 2013) to minimize contamination and improve yield. Quality and concentration were evaluated in-house through gel electrophoresis and a NanoDrop ND-1000. Total RNA was sent to Novogene Corporation Inc., where an Aligent 2100 BioAnalyzer (Aligent Technologies) confirmed the RNA integrity and quantification, library preparation was performed (Eukaryotic mRNA nondirectional library-NEB) and high-throughput Illumina sequencing (HiSeq2500, 150 base pair (bp), paired-end reads) was performed on all the samples yielding ~20M raw reads/sample (Figure 1b). The Novogene bioinformatics team removed the Illumina adaptor sequences and trimmed any low-quality reads prior to delivering the sequences.