Dsim
We extracted DNA from 30 virgin 0-day males using DNeasy Blood & Tissue Kit and diluted in 100 L ddH2O. The DNA was then treated with 1 L 10mg/mL RNaseA (Invitrogen) at 37C for 1-hr and was re-diluted in 100 L ddH2O after ethanol precipitation. The size and concentration of DNA was analyzed by gel electrophoresis, Nanodrop, Qubit and Genomic DNA ScreenTape. Finally, we constructed the libraries using PCR-free standard Illumina kit and sequenced 125-bp paired reads with a 550-bp insert size from the libraries using Hiseq 2500 in UR Genomics Research Center.