RNA-Seq of homo sapiens: breast
The RNA obtained after extraction was completed for concentration measurement using Nanodrop 2000C (Thermo Fisher Scientific, USA), and Agilent 2100 Bioanalyzer (Thermo Fisher Scientific, USA) was applied to RNA fragment integrity analysis. Eligible RNA samples were subjected to library construction using the VAHTS Total RNA-seq (H/M/R) Library Prep kit for Illumina (NR603-02) strand-specific method. Concentration measurements were performed with Qubit4.0 Fluorometric Quantitation (Thermo Fisher Scientific, USA), and RNA library fragment analysis was performed with an Agilent 2100 Bioanalyzer and High Sensitivity DNA Reagents/Chips (Agilent, 5067-4626). Qualified libraries were sequenced in PE150 mode on the Illumina HiseqX platform (Illumina US).