GSM6280579: SARS-CoV-2.1; Homo sapiens; RNA-Seq
Total RNA from HCV/ SARS-CoV-2 monoinfected, coinfected and non-infected Huh7.5 cells (generated as described above) was purified using the RNeasy Mini Kit (Qiagene). 1 μg of total RNA was treated with the NEBNext poly (A) mRNA Magnetic Isolation Module (NEB). RNA-seq libraries were produced using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB). For each tested group three biological replicates were performed.