GSM5552452: bovine milk extracellular vesicles-rep3; Bos taurus; miRNA-Seq
Extracellular vesicles were separated using a novel salting-out method. RNA was harvested from extracellular vesicles using Trizol reagent (Invitrogen/Life Technologies). RNA libraries were constructed using QIAseq miRNA Library Kit (Qiagen, Hilden, Germany) following the manufacturer's instructions. Individual libraries were constructed using 100ng of total RNA isolated from each sample.