GSM5354722: L4 _2; Caenorhabditis elegans; RNA-Seq
Well synchronized worms were washed at least 3 times to remove the bacteria from NGM plates , flash frozen on liquide Nitrogen, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit  was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols