GSM5585061: HT-22 Mouse hippocampal neuronal cell- Glutamate with PP treatment 24h; Hippocampus; RNA-Seq
Total RNA extracted using TRIzol reagent according to the manufacturer's instructions. Briefly, 1 mL of TRIzol lysate was mixed with 200 µL of ice-cold chloroform and centrifuged at 12,000 × g at 4°C for 15 min. Then, 400 µL of the aqueous phase was transferred and mixed with 500 µL of isopropyl alcohol, incubated for 15 min -20°C, and centrifuged at 12,000 × g at 4°C for 10 min. The resulting pellet was washed three times with 75% ethanol and centrifuged at 7,500 × g at 4°C for 5 min. Finally, the RNA pellet was dried at room temperature for 30 min before resuspension in 30 µL of RNase-free water. The amount of RNA was detected using a NanoDrop spectrophotometer at 260 nm.