GSM5699327: HOM1_CMP; Mus musculus; RNA-Seq
Total sample RNA was extracted using Trizol with Genelute LPA (Sigma) as a carrier and SMARTer Ultra Low RNA kit for Illumina Sequencing (Clontech) was used for cDNA synthesis according to the manufacturer's protocol cDNA was sheared with the Covaris device and processed according to the TruSeq RNA Sample Preparation v2 Guide (Illumina). Amplified sample libraries were subjected to paired-end sequencing (2 x 75 bp).