GSM4730321: SLX12477_INF_mouse1_scRNA-seq; Mus musculus; RNA-Seq
Bones were crushed in PBS with 2% fetal bovine serum (2% FBS) and the cells filtered through a 40µm strainer, depleted of red blood cells and stained with relevant antibodies. Cells were washed in PBS with 0.04% BSA and re-suspended before loading 10,000 cells onto each channel of the Chromium 10x Genomics platform to capture single cells in droplets. Library generation for 10x Genomics v2 chemistry was performed following the Chromium Single Cell 3ʹ Reagents Kits User Guide: CG00052. Quantification of cDNA was performed using Qubit dsDNA HS Assay Kit (Life Technologies Q32851) and high-sensitivity DNA tape-station (Agilent. 5067-5584). Quantification of library construction was performed using Qubit dsDNA HS Assay Kit (Life Technologies Q32851) and high-sensitivity DNA tape-station (Agilent. 5067-5584).