NodD metabarcoding of Rlv populations
The nodD309 barcode PCR amplification was achieved using nodD309MiSeq primers and genomic DNA extracted from symbiotic roots. Multiplexing was performed using a homemade 6 bp index, which was added to the amplicons during a second PCR with 12 cycles using Miseq_round2 specific primers. Purified PCR products were loaded onto the Illumina MiSeq cartridge (Miseq V3 kit). Details in Boivin et al. (Frontiers in Plant Science, submitted).