PacBio long-read sequencing of Peromyscus maniculatus rubidus
high-molecular weight DNA was extracted from whole blood using MagAttract HMW DNA mini kit (Qiagen) and then libraries were prepared with the SMRTbell Express Template Prep Kit 2.0 (PacBio), with size selection of 30-kb using BluePippin (Sage Science), subreads bam was converted to fastq file using bam2fastx (PacBio)