GSM6281226: wild-type hok/Sok, replicate 1; Escherichia coli; OTHER
The genomic DNA of bacterial colonies was extracted using the Quick Bacteria Genomic DNA extraction Kit (Nebiotech) following the supplier's recommendations. After genomic DNA extraction, the hok/Sok toxin-antitoxin locus was amplified with a specific primer pair generating an amplicon of 486 nt. The primers carried an overhang 5'-tail containing the adaptors for Illumina sequencing. Sequencing of the amplicon libraries was done on a MiSeq instrument in paired-end mode 2 x 300 nt (overlapping reads) at the Bordeaux Transcriptome Genome Platform, Cestas Pierroton, France.