Encarsia suzannae female transcriptome
Total RNA was extracted from single-sex pools of wasps. RNA was isolated immediately after wasps were shock frozen. For RNA isolation, the Trizol reagent (Invitrogen) was used, and contaminating genomic DNA was digested with the Turbo DNA-free kit (Ambion) according to the manufacturer's instructions. Samples were subjected to standard Illumina library preparation using the NEBNext Ultra RNA library prep kit according to the manufacturer's instructions. Six double-stranded cDNA libraries, three for each sex, were single end sequenced (50 bp) using an Illumina HiSeq2500 machine.