GSM5585252: Oxytricha WT DIP - native; Oxytricha trifallax; OTHER
Genomic DNA was extrated using DNeasy Blood and Tissue Kit according to the manufacturer's instructions (Qiagen, 69506) Genomic DNA was fragmentized to 100-300 bp using Covaris S220 Focused-ultrasonicator. Fragmented gDNA was ligated to TruSeq adaptor using NEBNext Ultra™ II DNA Library Prep Kit for Illumina (NEB, E7645S). 6mA DIP enrichemnt was performed using anti-6mA antibody (CST, D9D9W). Unmodified control DNA was made by amplifying DIP enriched DNA using Q5 DNA polymerase (NEB, M0492S). Nitrite treatment was performed with both native and amplified DNA. Indexed librarier were constructed using NEB ultra ii library prep kit.