GSM5857684: HT29 adherent-Con-2; Homo sapiens; RNA-Seq
Isolation of total RNA was accomplished using the miRNeasy kit (Zymo Research, Irvine, CA) according to manufacturer's instructions. RNA quality was determined by an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA) and samples with RNA integrity numbers (RIN) > 7 were used. paired-end sequencing of 75bp reads on Iillumina NextSeq