WGS of Canis lupus familiaris: sample DOGPT30
the genomic DNA is randomly fragmented to size of 350bp. DNA fragments were end polished, A-tailed, ligated with adapters, size selected and further PCR enriched. Then PCR products were purified (AMPure XP system), followed by size distribution by Agilent 2100 Bioanalyzer (Agilent Technologies, CA, USA), and quantification using real-time PCR. Library was then sequenced on NovaSeq 6000 S4 flow cell with PE150 strategy