GSM5465303: HASMCs, siBAF60c_H3K27ac; Homo sapiens; ChIP-Seq
Chromatin immunoprecipitation was performed with the SimpleChIP Enzymatic Chromatin IP Kit (Cell Signaling, #9003), according to the manufacturer's recommendation. Histone-DNA complexes were isolated with antibodies against H3K9ac (Cell Signaling, #9649, 1:100 dilution), H3K27ac (Cell Signaling, #8173, 1:100 dilution), H3K9me2 (Cell Signaling, #4658, 1:100 dilution), H3K27me3 (Cell Signaling, #9733, 1:100 dilution) or BRG1 (Abcam, ab110641, 1:100 dilution). The sample for BRG1 Chip-seq was crosslinked using 2mM disuccinimidyl glutarate (DSG, ThermoFisher Scientific, #20593) at room temperature for 45min before paraformaldehyde crosslinking. The DNA library preparation and sequencing were performed by the DNA sequencing core at the University of Michigan. Briefly, the DNA library was prepared with NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA) and sequenced on a NovaSeq 6000 S1 flowcell (Illumina) with100bp pair-end reads.