GSM5651332: ex vivo prostate epithelial cells - K_rep5; Mus musculus; RNA-Seq
Prostates were removed, single cell suspensions were prepared and EpCAM positive epithelial cells were enriched using a magnetic bead based sorting approach. RNA was harvested using ReliaPrep RNA Tissue Miniprep Kit (Promega). For library preparation, the Ultra II Directional RNA Library Prep Kit (E7760, NEB) was used in combination with a poly(A) mRNA magnetic isolation module (E7490). 250ng total RNA were used. RNA libraries were prepared for sequencing using standard Illumina protocols