ATAT-seq of mus musculus: adult female spleen
Transferred P14 cells were isolated from spleen of donor mice infected with LCMV Armstrong. The unfixed nuclei of these cells were tagged using Tn5 transposase (TruePrep DNA Library Prep Kit V2 for Illumina) for 30 min at 37  for library selection, and the resulting library fragments were generated by 10-12 PCR cycles, and finally sequenced on an Illumina Novaseq-PE150.