GSM5353045: H3K27ac_dBMDM_M1_1; Mus musculus; ChIP-Seq
1 x 10^6 cells/mL were fixed with 1% formaldehyde at room temperature for 10 minutes, washed with PBS and stored at -80oC. Cells were sheared using the Chromatin EasyShear Kit – Low SDS (Diagenode, Belgium) and sonicated using a Bioruptor® Pico sonication device (Cat# B01080010) (Diagenode, Belgium) according to the manufacture's recommendations. ChIP-seq libraries were prepared with the NEBNext Ultra II kit (New England Biolabs). Library amounts were quantified using the Qubit 2.0 Fluorometric Quantitation system (Life Technologies) and the size distribution was assessed using the Experion Automated Electrophoresis System (Bio-Rad). For sequencing, libraries were pooled, diluted and sequenced on an Illumina HiSeq 3000 instrument using 50 bp single-read chemistry.