GSM4731439: MMAR_0500_IP; Mycobacterium marinum; ChIP-Seq
Lysates were clarified from sonicated genomic DNA and the FdmR protein-DNA complexes were isolated with antibody. The DNA of input and IP was extracted by phenol-chloroform method. The high-throughput DNA sequencing libraries were prepared by using VAHTS Universal DNA Library Prep Kit for Illumina V3 (Catalog NO. ND607, Vazyme). The library products corresponding to 200-500 bps were enriched, quantified and finally sequenced on Novaseq 6000 sequencer (Illumina) with PE150 model.