GSM5310252: WT input rep 1; Homo sapiens; OTHER
Cells were lysed in Trizol (Invitrogen) and total RNA was purified by chloroform extraction followed by isopropanol precipitation. Ribosomal RNA depletion was performed using the RiboMinus Eukaryote Kit v2 (Life Technologies) per manufacturer instructions. Total RNA was fragmented using the Bioruptor Pico sonicator (Diagenode) 30 sec on, 30 sec off, 30 cycles. m6A immunoprecipitation was performed using the EpiMark N6-methyladenosine Enrichment Kit (NEB) as per manufacturer's instructions. Input at m6A IP samples were used to create libraries using the SMARTer Stranded Total RNA-seq Kit v2 (Takara) as per manufacturer's instructions.