GSM6336872: Sample-16, Nurr2c; Mus musculus; RNA-Seq
Using a 0.5 mm biopsy punch and a dissecting microscope, habenula samples were collected from two 400 um slabs made on a Leica 1850 CM cryostat. From habnenula biopsy punches, single nuclei were extracted into droplets containing barcoded GEMs (Gel Bead-In Emulsions) using the 10X v3.1 3' snRNA-seq kit.  After a cleanup step, cDNA were amplified, and libraries were constructed for sequencing.