GSM5101188: 1_PF382_control_1; Homo sapiens; RNA-Seq
Total RNA was extracted from 4 ×106 cells using the RNeasy Mini Kit (Qiagen; cat. 74104). RNA concentration was determined by a NanoDrop 2000 spectrophotometer (ThermoFisher Scientific), and RNA quality was assessed with an Agilent Bioanalyzer. Approximately 100 million reads per sample were run on an Illumina Nextseq 500 system using 75 × 75 paired end (PE) reads for stranded whole-transcriptome sequencing The barcoding reaction was run using the NEBNext HiFi kit on the basis of the manufacturer's instructions and amplified for five cycles