GSM5464598: ht-lib1_10: mc903_resident_mc; Mus musculus; RNA-Seq
Up to 100 cells of resident mast cells and integrinβ7+ MCs from both ears of NT mice and MC903-treated mice were collected into CDS sorting solution (Takara Bio Inc, Shiga, Japan) using 96 well plate. Then cDNA was synthesized using SMART-Seq HT (Takara Bio Inc) protocol, and purified using Agencourt AMPure XP (Beckman Coulter). RNA-seq libraries were prepared using Illumina Nextera XT DNA Library Prep Kit (Illumina, San Diego, CA)