GSM5651637: ATRX WT cells, untreated, replicate 1; Homo sapiens; RNA-Seq
Total RNA was extracted using Trizol following manufacturer's instructions. RNA was treated with Turbo DNA-free kit (life technologies) and quality checked with TapeStation (Agilent). Sequencing library preparation was then completed using the QuantSeq 3'-mRNA kit (Lexogen) to generate Illumina-compatible sequencing libraries according to the manufacturer's instructions