GSM5914107: 32215_cornea [Sample_5]; Oryctolagus cuniculus; RNA-Seq
Tissues were homogenized using a Mini-Bead beater 16 (Biospec Products) at 10 °C. Samples were homogenized for approximately 90 seconds and then cooled for 90 seconds on ice for three rounds. Following homogenization, RNA was extracted using a RNeasy Extraction kit (Qiagen, Germantown, MD), according to the manufacturer's protocol, with an additional wash step of 500 microliters of Buffer RPE prior to adding the RNAse-free water. Following this step, 50 microliters of RNAse-free water was added to the spin-column membrane to extract the total RNA. Total RNA samples were sent to BGI Group for quality control verification and library preparation. Library constructed by BGI using DNBseq platform.