GSM6280833: M1, scRNAseq; Homo sapiens; RNA-Seq
There was a lysis component and an enzyme inhibitor in the tubes used to collect single cell samples. We then used the Smart Seq2 technique for amplification. The initial strand of cDNA was synthesized using an Oligo dT primer, which was then amplified using PCR and purified using magbeads. Qubit 3.0 Fluorometer and Agilent 2100 Bioanalyzer were used to verify that the predicted 1–2kbp cDNA synthesis had occurred. Ultrasonic waves were used to randomly shear the cDNA in preparation for the Illumina library preparation technique, which includes DNA fragmentation, end repair, 3' end A tailing, adaptor ligation, PCR amplification, and library validation.