GSM4826134: spt4del_Spt5_TEF-seq_rep2; Saccharomyces cerevisiae; OTHER
Cells were filtered and flash frozen in liquid nitrogen. 4 parts of the frozen S.cerevisiae pellet were spiked-in with one part of frozen S.pombe (containing a FLAG-tagged Rpb9) pellet (4:1 by weight). The combined pellet was lysed, and immunoprecipitation of the FLAG-tagged proteins was performed at 4°C as described in (Fischl et al., 2017). RNA was isolated with Qiagen miRNA kit according to the manufacturer's instructions. The isolated RNA was adapter ligated, fragmented, reverse transcribed, circularized, barcoded, and amplified as described in (Fischl et al., 2017).