GSM6280499: Cortex, WT, rep1; Mus musculus; RNA-Seq
For RNA extraction, TRIzol was added to the tissue powder and homogenized with Dounce tissue homogenizer. The RNA was treated with TurboDNase (Invitrogen, AM2238) to remove genomic DNA contamination. For peripheral tissues and N2a cells, total RNA was extracted and the integrity analyzed by a fragment analyzer. For peripheral tissues and N2a cells, total RNA was extracted and the integrity analyzed by a fragment analyzer. Library preparation and RNA sequencing were performed by Novogene (CA, USA). For brain samples, polyadenylated mRNA was enriched using Nextflex Poly(A) Beads (NEXTflex, Bioo Scientific Corp, 512980) and cDNA libraries were prepared using a NEXTflex Rapid Directional qRNA-Seq Kit (Bioo Scientific Corp, NOVA-5130-03D).