K.pneumoniae CFN_9448
Sequential bacterial isolates recovered from positive blood cultures were identified retrospectively. Isolates were subcultured from frozen stocks twice on tryptic soy agar with 5% sheep blood (BD Diagnostics, Sparks, MD, Phase 2) for 18-24h at 37C. DNA extraction was performed using the DNeasy PowerSoil Pro Kit (QIAGEN). The Rapid Barcoding Kit (SQK-RBK004) was used for library prep using 400 ng DNA input per sample and following the manufacturers instructions. Sequencing was done on R10.3 flow cells on a GridION.