GSM6281078: Wild type mRNA; Saccharomyces cerevisiae; RNA-Seq
Cells were mixermilled with frozen buffer (20mM Tris pH8, 140mM KCl, 1.5mM MgCl2, 100ug/ml cycloheximide, 1% Trition X-100), buffer was thawed, spun at 3Kg  for 5 minutes, then supernatatant was spun at 20Kg for 10 minutes. As described in Ingolia et al, Science 2009, except using the linker ligation strategy in place of polyA tailing and rRNA subtraction, both from Ingolia et al, Nature Protocols 2012