GSM5857868: WNT2; Mus musculus; RNA-Seq
Tissues were flash frozen on dry ice, and total RNA was harvested using Trizol reagent. All samples need to pass through the following four steps before library construction: (1) Nanodrop: tests RNA purity (OD260/OD280)  (2) Agarose Gel Electrophoresis: tests RNA degradation and potential contamination (3) Agilent 2100: checks RNA integrity RNA libraries were prepared for sequencing using standard Illumina protocols