GSM5857270: MEFs, EZR KO, biological rep4; Mus musculus; RNA-Seq
RNA was quantified and mixed at 10ng/µl libraries were prepared according manufacturer's instructions (TruSeq RNA Sample Preparation kit, Illumina) starting from 4 μg of total RNA. Quality control of library templates was performed using a High Sensitivity DNA Assay kit (Agilent Technologies) on a Bioanalyzer (Agilent Technologies). The Qubit quantification platform (Qubit 2.0 Fluorometer, Life Technologies) was used to normalise samples for the library preparation.