GSM5229946: pcDNA4c Control 2; Mus musculus; RNA-Seq
Electroporated areas of the retinas were collected at postnatal day 8 and disassociated with papain solution. Intact GFP positive rod photoreceptors were manually collected with a micromanipulator. Kit v2-Pico Input Mammalian (Takara Bio USA, Inc). We prepared three independent RNA samples for each treatment and then performed paired-end sequencing with a HiSeq2500 (Illumina, San Diego, USA). Libraries were generated from rod cells using SMARTer Stranded Total RNA-Seq