16s rRNA amplicon sequencing of mus musculus: feces
For DNA extraction, Quick-DNA Fecal/Soil Microbe kit column-based, bead beating step included. In triplicate, the V4 region of the 16S rRNA gene was amplified with barcoded PCR primers F515 and R806 modified to contain an adapter region for sequencing on the Illumina MiSeq platform. Amplicons were verified by gel electrophoresis, combined, purified, and sent to the UC Davis Genome Center for library preparation and high throughput 250-bp paired-end sequencing using the Illumina MiSeq platform.