Human vaginal microbiota, sample Sample_6
Nucleic acids were extracted from vaginal swabs by means of DNeasy Blood & Tissue Kit (QIAGEN), following the protocol for Gram-positive bacteria. The V3-V4 hypervariable regions of the bacterial 16S rRNA gene were amplified, according to the 16S metagenomic sequencing library preparation protocol (Illumina, San Diego, CA, USA). Final indexed libraries were prepared by equimolar (4nmol/L) pooling, denaturation, and dilution to 6pmol/L before loading onto the MiSeq flow cell (Illumina). A 2300bp paired-end run was used.