GSM5006399: B1 Cytosol Control; Homo sapiens; RNA-Seq
HeLa cell fractions were extracted in guanidinium thiocyanate-phenol-chloroform and total RNA was isolated. Following biotinylation, streptavidin affinity isolated of 4SU RNA, and oligo-dT selection for 4SU-poly(A) mRNA, mRNAs was processed for deep sequencing using NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (New England Biolabs, E7770).  NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, E7490) was used for poly(A) selection. libraries were constructed using NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (New England Biolabs, E7770)