GSM5552562: TX1.ChIP.rep3; Cryptococcus neoformans var. grubii H99; ChIP-Seq
Cells were poured into 200 ml conical flasks containing 1.39 ml of 37% formaldehyde up to the 50 ml mark and incubated with gentle rocking for 15 min at room temperature, after which 2.7 ml of 2.5M glycine was added to stop the crosslinking and cells were mixed for 5 min. Cells were harvested at 3000 rpm for 5min at 4 °C, washed twice with ice-cold PBS containing 125mM glycine. Chromatin was extracted as described previously(Li et al., 2019). The ChIP-seq library was generated using the MicroPlex Library Preparation Kit v2 (Diagenode, Liège, Belgium) according to the manufacturer's instructions. The ChIP DNA libraries were sequenced using an Illumina Hiseq-PE150 Platform.