Oncomine Focus panel of GM12877 cell line sequenced on Ion Torrent PGM
Treatment, growth and extraction protocols:  Cells lines were cultured according to manufacturers protocol.  Cells were harvested in aliquots of 30 million cells and submitted to EORLA to be processed into FFPE blocks.  DNA  was extracted sequentially from the same sample using AllPrep DNA FFPE (Qiagen) from 5 FFPE tissue block curls. Sample deparaffinization was performed with 900 ul of mineral oil and 1 min incubation at 80C before proceeding to AllPrep procedure starting from step 5.   Library Preparation  Library prep was performed according to manufacturers protocol with the input amounts specified below:  Oncomine Focus on PGM (5 ng input DNA)