GSM5353198: HT29 YB-0158 0.3uM #2; Homo sapiens; RNA-Seq
RNA was extracted using the total RNA purification kit by Norgen Biotech Corp, following the manufacturer's guidelines. Quantification of total RNA samples was performed with a Qubit HS RNA assay (ThermoFisher Scientific) and fragment size was evaluated with a Fragment Analyzer HS NGS assay (AATI). A RNA Quality Number (RQN) of 8.0 or higher was considered satisf.txtactory for library construction. Library construction was performed with a Truseq RNA v2 (Illumina) with unique barcodes compatible with the Illumina NextSeq 500 platform. RNA libraries were prepared for sequencing using standard Illumina protocols