GSM5206877: P.N.61; Mus musculus; RNA-Seq
Whole hippocampal mRNA was extracted from flash frozen tissue using the RNeasy Lipid Tissue Mini Kit (Qiagen; Catalog # 74804). RNA concentration and quality were determined using a Bioanalyzer (Agilent). Samples with a RIN > 9 (RNA Integrity Number) were used for sequencing (WT-N, n = 6; WT-CIH, n = 6; P301S-N, n = 5; P301S-CIH, n = 6). Sequencing libraries were prepared using the TrueSeq Stranded mRNA Kit (Illumina) by the GENEWIZ (South Plainfield, NJ, USA). Libraries were barcoded to allow for multiplexing in a single flow cell. 100 bp paired- end reads were collected on a HiSeq 2500 (Illumina) at a sequencing depth of ~40 million reads per sample.