GSM5397370: WT_2_techRep1; Mus musculus; RNA-Seq
Effector OT-I or OT-I/TGFbRII-/- cells were injected into HSV-infected mice and on day 14, skin was harvested from perfused mice and subject to a dispase digestion. This allowed the physical separation of the epidermis from the dermis skin. Epidermal skin was further digested in collagenase,  and the cell suspension was stained for sort purification on OT-I markers. A total of 500 cells were sorted into individual strip tubes containing buffer comprised of dNTPs and BSA. Libraries were prepared with smartseq-nextera2 kit according to manufacturers instructions