RNA-Seq of biofilm3
Total genomic DNA of MPs-associated biofilms, filtered water membrane and sediments was extracted from samples according to the standard operating procedures of E.Z.N.A. soil DNA Kit (Omega Bio-tek, Norcross, GA, U.S.). The hypervariable region V3-V4 of the bacterial 16S rRNA gene were amplified with primer pairs 338F (5- ACTCCTACGGGAGGCAGCAG-3) and 806R (5-GGACTACHVGGGTWTCTAAT-3) by an ABI GeneAmp 9700 PCR thermocycler (ABI, CA, USA).