Shotgun sequencing of Florida's Indian River Lagoon (USA): Site CCHH-03
Approximately 17 ng of mDNA from each water sample was used to construct libraries with an insert size of 300 bp. Illumina paired-end sequencing was performed on the iSeq100 platform at the FAU Harbor Branch Oceanographic Institute (Ft. Pierce, Florida. U.S.A.) using two-dye sequencing by synthesis chemistry and Nextera libraries, producing 4 M paired-end (2 x 150-bp PE) reads per run. Resequencing was performed on samples that produced less than ~1 M reads, and all sequences were used for downstream analysis.