GSM5962128: Patient_5514_ib [S11]; Homo sapiens; RNA-Seq
Using RNeasy Mini Kit (QIAGEN, CAT NO 74104) to extract and prepare RNA for RNAseq. The extraction process followed protocol of product. The sequencing libraries were constructed from 100 ng – 500 ng of total RNA using the Illumina's TruSeq Stranded Total RNA kit with Ribo-Zero (Cat# 20040529) following the manufacturer instruction. The fragment size of RNAseq libraries was verified using the Agilent 2100 Bioanalyzer (Agilent) and the concentrations were determined using Qubit instrument (LifeTech). The libraries were loaded onto Illumina Novaseq 6000 for 2x50 bp paired end read sequencing. The fastq files were generated using the bcl2fastq software for further analysis.