GSM5396572: RS-B/RNAseq; Brassica napus; RNA-Seq
Total RNAs were extracted from 1/10th of the lysates used for Ribo-Seq analysis (see GEO Submission (GSE178543)) and treated with the Ribo-Zero rRNA Removal Kit (Illumina). One hundred ng of the rRNA-depleted RNAs were used for library construction using the NEXTflex Rapid Directional qRNA-Seq Kit (Bioo Scientific) according the manufacturer's instructions. Next generation sequencing was performed on a NextSeq 500 sequencer (single end, 75 nt). RNA libraries were prepared for sequencing using standard Illumina protocols