GSM4826822: Panc_PDX_18269_LDE225_7d_rep1; Homo sapiens; RNA-Seq
Total RNA was extracted using RNeasy Mini kit (Qiagen, Germantown, MD) according to the manufacturer's instructions, and included RNase inhibitor (N808-0119, Applied Biosystems, Waltham, MA). RNA-Seq libraries from 1μg RNA/sample (low-sample protocol of TruSeq Stranded mRNA Library Prep Kit, Illumina, San Diego, CA) were indexed using an automated IP-Star Compact Liquid Handler (Diagenode, Belgium) amplified, quantified, pooled, and sequenced to yield 25-30M single-end 80BP reads per sample on an Nextseq 500 (Illumina).