GSM5101938: N2a KO vehicle Ribo-seq rep1; Mus musculus; OTHER
For RNA-seq, total RNA was extracted using TRIzol reagent as described by the manufacturer. For Ribo-seq, cells were lysed in polysome lysis buffer as described in Weiss et al 2021. For RNA-seq, libraries were prepared following Illumina Truseq Stranded mRNA kit. For Ribo-seq, homemade protocol was adapted from Ingolia et al 2012 and is available at Weiss et al 2021.