GSM5897899: MUN-013 T2; Drosophila melanogaster; miRNA-Seq
Total RNA was isolated using Trizol (Thermo Fisher Scientific) from three replicates of 4-7 day-old flies after desiccation stress and in control conditions. For the extraction 30 whole female flies were used per replicate. RNA samples were treated with DNAse I (Thermo Fisher Scientific) following manufacturer's instructions. small RNAs were obtained by gel size selection from total RNA using 10% TBE urea gel (Thermo Fisher Scientific). Fragments between 17 and 30 nucleotides were carefully cut from the gel and were eluted for subsequent sequencing (Quiagen elution kit). Quality check was performed using Bioanalyzer small RNA kit. Libraries were sequenced using Illumina 50 bp single-end reads. Libraries were done using standard Illumina library prep kits.