GSM5912643: micu_rep3; Caenorhabditis elegans; RNA-Seq
Adult nematodes were dissolved in 1mL Trizol and total RNA was isolated via the RNeasy Micro kit. RNA concentration ad integrity were determined by High Sensitivity RNA assay on a TapeStation 4200 system. cDNA libraries were prepared from 5ng total RNA using the SMART-seq2 protocol and tagmented with the Nextera XT kit. Libraries were sequenced SR 75 cycles on a NextSeq500 system (illumina) using High Output v2 chemistry. RNA libraries were prepared for sequencing using standard Illumina protocols