GSM6336793: Control-2; Homo sapiens; RNA-Seq
Total RNA was isolated from formalin-fixed, paraffin-embedded (FFPE) lung tissues of seven patients and three non-covid infected individuals using the Maxwell® RSC RNA FFPE Kit in Maxwell® RSC Instrument (Promega, Dübendorf, Switzerland).  The quantity and quality of the extracted RNA was assessed using a Thermo Fisher Scientific Qubit 4.0 fluorometer with the Qubit RNA BR Assay Kit (Thermo Fisher Scientific, Q10211) and an Advanced Analytical Fragment Analyzer System using a Fragment Analyzer RNA Kit (Agilent, DNF-471), respectively. Ribosomal RNA was depleted from 100 ng of total RNA samples using a RiboCop for Human/Mouse/Rat (HMR) V2 kit (Lexogen,144) according to the vendors guidelines. Thereafter, sequencing libraries were produced using a Swift RNA Library Kit (Swift Biosciences, 10009985) in combination Swift Unique Dual Indexing Primers (Swift Biosciences, 10009912) and according to a technical note developed by Swift Biosciences entitled "The Swift RNA Library Kit Optimizes RNA-Seq Data Quality and Costs for FFPE RNA".