GSM5464858: ActD_0hr_RNA_rep1; Homo sapiens; RNA-Seq
Total RNA was extracted using Trizol (Invitrogen). For RNA sequencing, an equal amount of ERCC RNA spike-in control (Thermo Scientific, Waltham, MA) was added to the total RNA samples as internal control before library construction. Sequencing libraries were prepared using NEBNext Ultra Directional RNA Library Prep Kit. RNA stability assay was generated from two biological replicates.