GSM5006471: DON_1; Homo sapiens; RNA-Seq
Total RNA was extracted from Caco-2 cells using Trizol reagent. The concentration and purity of the RNA samples were examined at 260/280 nm ratio by Nanodrop. RNA degradation and contamination were monitored on 1% agarose gel electrophoresis (AGE) by Mini-Sub Cell GT System. RNA libraries were prepared for sequencing using standard Illumina protocols