NextSeq 500 sequencing; Effect of IL-17R in Paneth cells after DSS challenge
Effect of IL-17R in Paneth cells after DSS challenge
Mice were killed by cervical dislocation followed by dissection and ilea harvesting. Before ilea extraction, mice were administered with DSS treatment: 7 days DSS, 10 days of normal water, 7 days DSS. RNA was isolated using RNEasy mini kit (QIAGEN). Sequencing libraries were prepared using mRNA Hyperprep Kit (KAPA).