NGS P. putida RB-TnSeq library: M9 + 20 mM D-glucose enrichment from M9 + 20 mM D-glucose T=0 Replicate A
1 mL cell pellet used to extract gDNA by the GeneJet Genomic DNA Purification Kit (Thermo Scientific, USA). Specific primers used to amplify the Tc1 associated barcode region with Q5 High-Fidelity 2X Master Mix (New England Biolabs, USA). Samples pooled and sent to Genewiz for sequencing