PacBio Sequencing of Leishmania amazonensis PH8 strain: promastigore cultures
Genomic DNA (gDNA) was obtained from promastigote cultures of L. amazonensis PH8 strain, isolated from Lutzomyia flaviscutellata in the city of Belm, Brazil. The extraction and purification of gDNA was performed using Life Technologies DNA extraction kit. and sequenced using TruSeq RNA and DNA Library Preparation Kit v2 to produce 350 bp-size paired-end reads on the Illumina Hiseq 2000 platform.