GSM5699310: PS_male_amygdala_LM27; Mus musculus; RNA-Seq
PN28 mice were sacrificed via cervical dislocations and brains were harvested, immediately frozen on dry ice, and stored at -80⁰C. Frozen brains were then submerged in RNAlater-ICE (Cat# AM7030) and allowed to thaw overnight at -20⁰C per manufacturer's protocol. Amygdalar dissections were performed under a dissecting microscope. RNA was purified using RNeasy Micro Kit (Qiagen, Cat# 74004) following manufacturer's instructions. PolyA RNA was isolated using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England BioLabs, Cat# E7490) with a total of 1 µg of good quality total RNA as input and enriched using the SMARTer Apollo NGS library prep system (Takara Bio USA). Sequencing libraries were prepared using the NEBNext Ultr II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Cat# E7760). RNA libraries were prepared for sequencing using standard Illumina protocols, using the NEBNext Ultr II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Cat# E7760).