WES of Mus musculus: B cell lymphoma
Mouse exomes were individually prepared using 200 ng of DNA, the standard protocol SureSelectXT Automated Target Enrichment for Illumina Paired-End Multiplexed Sequencing, and the Agilent Bravo automated liquid handling platform. After validation (2200 TapeStation; Agilent Technologies) and quantification (Qubit System; Invitrogen, Waltham, USA) pools of libraries were generated. The pools were quantified using the KAPA Library Quantification kit (Peqlab, Erlangen, Germany) and the 7900HT Sequence Detection System (Applied Biosystems, Waltham, USA) and subsequently sequenced on an Illumina NovaSeq6000 sequencing instrument using a paired-end 2100 bp protocol.