Iskra
DNA was isolated from the leaves and stems of young potato shoots grown on a growing medium. Leaves weighing ~100 mg were homogenized in 2 ml tubes filled with zirconium beads 1 mm in diameter using an MP-24 homogenizer. Extraction from homogenized leaves was done using the innuPREP Plant DNA Extraction Kit by Analytik Jena (Jena, Germany) in accordance with Protocol #3. At the stage of nucleic acid binding to the column, RNase Cocktail Enzyme Mix by Invitrogen (Carlsbad, CA, USA) was used to destroy RNA. The eluate was further purified by magnetic particles AMPure XP by Beckman Coulter (Bray, CA, USA ). Sequencing was performed in accordance with the protocol ONT 1D (SQK-LSK109) and Native Barcoding Expansion (EXP-NBD104, EXP-NBD114) FLO-MIN106 cell by Oxford Nanopore Technologies (Oxford, UK) on the sequencer MinION Mk1C.