Genomic sequencing of Aspergillus burnettii FRR 5400
A. burnettii was grown in liquid malt extract medium for 7 days with gentle shaking (40 rpm) at room temperature. DNA was extracted from mycelia (200 mg) using the PowerSoil DNA Isolation Kit (Mo BioLaboratories, Carlsbad, CA). For genomic sequencing, Nextera XT libraries were prepared using Illumina kits (Sample Prep Kit, Illumina, San Diego, CA), then subjected to quality control using a Bioanalyzer2100 (Agilent Technologies) before whole genome sequencing was carried out using 100 bp paired-end Illumina HiSeq 2000.