Whole Genome-seq of human patient_ALS:blood: Sample LP6008170-DNA_C03
Paired-end libraries are generated by automated liquid handlers using 500ng–1ug input gDNA and the Illumina DNA. Sample preparation HT Kit following protocol based on Illumina SeqLab DNA PCR-free library prep guide.  Pre-fragmentation gDNA cleanup is performed using paramagnetic sample purification beads (Agencourt AMPure XP reagents, Beckman Coulter). Samples are then fragmented, and libraries are size-selected following fragmentation and end-repair using paramagnetic sample purification beads to enrich for short insert sizes. Final libraries are quantified by qPCR and evaluated for quality using a gel electrophoretic separation system.  Following library quantitation, DNA libraries are denatured, diluted and clustered onto patterned flow cells using theIllumina cBot system. cBot runs are performed following cBot System Guide using Illumina HiSeq X HD Paired End Cluster Kit reagents. Clustered patterned flow cells are loaded onto HiSeq X instruments and sequenced on 151 bp paired-end, nonindexed runs. All samples are sequenced on independent lanes. Sequencing runs are performed based on the HiSeq X System Guide, using HiSeq X HD SBS Kit reagents. Illumina HiSeq Control Software (HCS), and Real-Time Analysis (RTA) are used with the HiSeq X© sequencers for real-time image analysis, and base calling.