sediment microbiota sample 1 collected from pond 2 in Sep, 2020
DNA extraction and high-throughput sequencing of 16S rDNA were performed as previously described. The V4-V5 hypervariable region of prokaryotic 16S rDNA was amplified using primers 515F and 909R. The amplicons were purified, quantified, and sequenced usin HiSeq 3000 platform_x0000__x0000__x0000__x0000_