Single cell RNA sequencing of the frontal cortex of Trem2-IPD transgenic mouse at 3 month of age
Cellular suspensions were loaded on a 10x Genomics Chromium Single Cell instrument to generate single cell GEMs. Single cell RNA-Seq libraries were prepared using GemCode Single Cell 3' Gel Bead and Library Kit. Sequencing libraries were loaded at 11pM on an Illumina HiSeq2500 with 2x50 paired-end kits using the following read length: 26 cycles Read1, 8 cycles i7 Index and 98 cycles Read2.