RNA-Seq of core active microorganisms in the Qing-Flavor Liquor fermentation
The mRNA was reverse-transcribed to cDNA and the cDNA was next used to synthesize U-labeled second-stranded DNA,A-base was then added to the blunt ends of each strand for ligation with the adapters with T-base. Single or dual-index adapters were ligated to the fragments,after U-labeled second-stranded DNA was treated with the heat-labile UDG enzyme, the ligated products were amplified with PCR,finally, paired-end sequencing was performed on an Illumina Hiseq 4000 at the LC Sceiences