RNA-Seq of Polycarpa mytiligera: CNS regeneration day 14
Samples were prepared at the IUI during September 2017. Fifteen P. mytiligera individuals (61 cm, from the tip of the oral siphon to the base of the animal) were used for neural complex regeneration experiments. Neural complex tissue samples were prepared for RNA extraction (RNAeasy mini kit from Quiagen) at different time points along the regeneration process. Total RNA was extracted following the manufacturers protocol. cDNA libraries were then prepared from high-quality samples (RNA integrity number (RIN) > 8) at the Weizmann Institute of Science, Life Sciences Core Facilities, Israel. Barcoded library samples were sequenced on an Illumina NextSeq 500 (2  150 bp) at Stanford University, CA, USA