adult male muscular
Total genomic DNA was extracted from the muscle tissue of the specimen using the CTAB method. Then, the DNA fragments were mechanically interrupted (ultrasound) into small fragments (~350 bp) for each sample, and the DNA fragments with prominent ends were repaired using a combination of 35 nucleic acid exonuclease and polymerase, with A added to the 33-en, and the sequencing joints were connected and PCR amplified to form sequencing libraries. The merged DNA was used to construct the sequencing library using NEBNextUltraDNA Library Prep Kit and sequenced on an Illumina NovaSeq 6000 sequencer. Each sample generated 150 bp raw data about 4.6GB. These reads sequences were bioinformatically sorted by barcode sequence and assembled into mitochondrial genomes using SPAdes version 3.14.0.