RNA-seq of Hs766T-L3
The Hs766T-L3 was a subline of Hs766T with stable liver metastatic ability, which was isolated from the liver of BALB/c nude mice. For generation of Hs766T liver metastatic cancer cells, we established a pancreatic cancer spontaneous liver metastasis model. Briefly, Hs766T cells stably expressed luciferase were injected into the pancreases of BALB/c nude mice. After 14 weeks, cancer cells that spontaneously metastasized to the liver were separated, expanded and reinoculated into the pancreases of next-generation recipients. After three generations of screening, the third generation of Hs766T cells (Hs766T-L3) developed liver metastasis in 9/10 mice. Hs766T-L3 cells were collected and expanded.. Then, RNA was extracted and subjected to RNA-seq.