RNA-Seq of Anagrus nilaparvatae: adult female and male bodys
150 adult wasps from male and female of A. nilaparvatae were used for RNA extraction. mRNA was enriched by magnetic beads with Oligo(dT) and broken into short fragments by Fragmentation buffer under high temperature. Using mRNA as template, the first cDNA strand was synthesized by adding six base random primers, and then synthesized second cDNA strand. The ends of the double-stranded cDNA were repaired and polyA was added to the 3 ends. The cDNA fragments with connectors were enriched by PCR amplification and sequenced by superior sequencing.