RNA-Seq of raw264.7 macrophages, treated with fructose
Briefly, total RNA of RAW264.7 cells infected with E. faecalis were extracted with a commercial RNA isolation kit (BioFlux), and mRNA were enriched by magnetic beads. For RNA-seq, the VAHTS Stranded mRNA-Seq Library Prep Kit (Vazyme) was used to construct strand-specific RNA-Seq libraries. After libraries qualified, the sequencing was conducted on the HiSeq 2500 platform.