16S of F3: soil
PCR amplification of the bacterial 16S rRNA genes V3-V4 region was performed using primers 338F/806R (ACTCCTACGGGAGGCAGCA/ GGACTACHVGGGTWTCTAAT). The purified amplicons were pooled in equal amounts, and performed using the Illlumina MiSeq PE278 platform under the guidance of users manual.