ChIP-seq of Fusarium graminearum: control of fng1 mutant
Hyphae samples were washed in cold PBS buffer and cross-linked with 1% formaldehyde for 10 minutes at room temperature and then quenched by addition of glycine (125 mmol/L final concentration).  Afterwards, samples were lysed and chromatins were obtained on ice.  Chromatins were sonicated to get soluble sheared chromatin (average DNA length of 200500 bp).  After immunoprecipitation with H4ac antibody, immunoprecipitated DNA was used to construct sequencing libraries and sequenced on Illumina Xten with PE 150 method.