ChIP against (GFP)-NFYA: experiment
In total 5 ng input and less than 500 pg of ChIP purified DNA were used to prepare paired-end sequencing libraries using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, E7645L) without size selection. The 75-nucleotide paired-end sequencing reads were generated (Illumina, HiSeq 3000) with 20-23 M reads per sample.