16S rRNA sequencing of microbiome from mouse spleen
Genomic DNA from Spleen of 8-week-old healthy BALB/c mice was extracted.V4 variable region of the 16S rRNA was amplified and was used to prepare the Illumina DNA library by following the Illumina TruSeq DNA library preparation protocol. Sequencing was performed on an Illumina MiSeq as per the manufacturers guidelines