16S of wastewater from constructed wetlands
PCR amplicons of variable region V3 of the 16S rRNA gene were generated essentially according to a published protocol using forward primer 341bf and reverse primer uni518r. Amplicons were created using the extracted DNA directly as template without prior multiple displacement amplification as in Vollmers et al. 2017. Universal illumina adapters were already included as primer overhangs. Sequencing library preparation and Paired-End sequencing (2x150 bp) were carried out using an Illumina Nextseq-System (Illumina, San Diego, CA, USA)