RNA-seq Paeruginosa:LB broth DMS3vir-infected replicate 2
The integrity of the total cellular RNA was evaluated using RNA tape of Agilent TapeStation 2200 before library preparation. All RNA samples were of high integrity with a RIN score of 7.0 or more. rRNA was first depleted for each sample using MICROBExpress Kit (AM1905, Fisher) following the manufactures instruction. The rRNA-depleted total RNA was subjected to library preparation using NEBNext Ultra II RNA Library Prep Kit (E7700, NEB) and barcoded with NEBNext Multiplex Oligos for Illumina (E7730, NEB) following the manufacturers instructions. The libraries were pooled with equal amount of moles, further sequenced using MiSeq Reagent V3 (MS-102-3003, Illumina) for pair-ended, 600bp reads, and de-multiplexed using the build-in bcl2fastq code in Illumina sequence analysis pipeline.