ONT of wheat VRN1:Muskat
The full-length sequence of three homoeologous VRN1 loci on chromosome 5A, 5B and 5D was amplified using a specific primer pair. The PCR amplicons were barcoded using the kit EXP-PBC096 (ONT Oxford, UK). The MinION library was produced using the Ligation Sequencing Kit 1D SQK-LSK109 (ONT Oxford, UK). Pooled library was loaded and sequenced on MinION R9.4.1 for approximately 24 hours.