PacBio sequencing of Myxobolus honghuensis spores DNA
Myxobolus honghuensis was collected from infected allogynogenetic gibel carp Carassius auratus gibelio in Zoumaling Farm, Hubei Province, China on July 29th, 2015. Genomic DNA was isolated and extracted using the CTAB method. Then, 10 ug gDNA was sheared to an ~20-kb targeted size using a Covaris g-Tube, then used for size selection using Blue Pippin (Sage Science, Beverly, MA, USA). 20-kb PacBio libraries were prepared following the standard PacBio protocol. It was sequenced on a PacBio RSII platform (PacBio Sequel sequencer at Biomarker Technologies Corporation) with P6-C4 chemistry.