16S rRNA V4 of Y Lake: 25m replicate A
Whole water samples were filtered on 0.22 m capsule filters (Millipore). The V4 region of the 16S rRNA gene was amplified by a 2-step PCR using the 515F forward primer, the 806R reverse primer and the Q5 High-fidelity polymerase (NEB). Amplicons were purified on sparQ PureMag beads (QuantaBio) and sequenced on an Illumina MiSeq by paired-end sequencing (2X300 pb).