mtDNA-seq of leaves from Nicotiana tabacum, point mutated at nad9, amino exchange D93N
After initial quality control using Agilent's Bioanalyzer sequencing libraries were prepared from 30ng of DNA per sample following Roche's  "KAPA DNA Hyper Prep Kit" library preparation protocol for double indexed Illumina libraries: First, DNA was sheared using a Covaris S2 system (duty cycle 5%, intensity 5, 40 seconds run time). After end repair and A-tailing Illumina sequencing compatible adapters carrying unique dual indices were ligated (NEXTFLEX Unique Dual Index Barcodes).  Following bead-based clean-up steps the libraries were amplified using 7 cycles of PCR. Library quality and size was checked with qBit, Agilent Bioanalyzer and qPCR. Sequencing was carried out on an Illumina MiSeq Nano flow cell in PE150bp mode yielding between 240,000-260,000 fragments per sample.