RNA-seq of Soil: Quercus velutina
A 250 bp portion of the V4 region of bacterial 16S rRNA gene was analyzed from each sample. Following PCR amplification, agarose gel verification, and standardization of samples using SequalPrep plates, the assembled library was sequened on Illuminia MiSeq at University of Mississippi Meical Center. Raw sequence files were processed using the DADA2 pipeline.