Golden Pompano Muscle Resequencing: Male Muscle
After genomic DNA is randomly broken into short DNA fragments with enzymes, blunt-end repair is performed. Then connect dA tails to both ends of the DNA fragments and connect sequencing adapters. The DNA fragments with adapters are purified by AMPure XP magnetic beads, and fragments in the range of 300-400bp are selected for PCR amplification. The established library was purified and library checked, and Hiseq X10 PE162 was sequenced on the computer.