L1
We evaluated the sperm motility phenotypic data of 200 boars by using a computer-assisted sperm analysis and selected eight Duroc boars to isolate extracellular vesicles (EVs). All the individual boars in the H group had higher total motility (>96%) than the boars in the L group (<79%), the percentages of fast-moving sperm in the H and L groups were > 72% and < 50%, respectively. For each boar, 35 mL of semen plasma was used to extract EVs. Deep RNA sequencing was performed to gain insight into the types of RNA in SPEVs, including mRNAs, miRNAs and lncRNAs.