RNA-Seq of rice germinated seeds:dry seed-2
For library construction,total RNA was isolated from the dry seeds using EASYspin Plus Complex Plant RNA Kit (Aidelai, China) according to the manufacturers instructions. 1.5 g of total RNA from each sample was used for rRNA removal using the Ribo-Zero rRNA Removal Kit (Epicentre, Madison, WI, USA). Sequencing libraries were generated using NEBNextR UltraTM Directional RNA Library Prep Kit for IlluminaR (NEB, USA) following manufacturers instructions.RNA libraries were prepared for sequencing using standard Illumina protocols.