Identification of novel viruses in anaplastic large cell lymphoma: Sample IA53_trimmed
"Total RNA libraries were prepared using the KAPA RNA HyperPrep Kit (Roche). Five samples (IA01, IA53, IA57, IA07, and IA17) were sequenced on a NextSeq 500 (Illumina) and the remainder on a NovSeq 6000 (Illumina). Forward and reverse read lengths were 150 bp. In order to detect potentially low viral loads, the targeted number of reads was 600 million per sample, and most samples had 400-800 million reads due to variation in loading estimates (Supplemental Table 1). Samples with fewer than 400 million reads had lower RNA input volumes due to lower sample quality."