RNA-Seq of Malus domestica: peel at CHD from Llesp mountain, biological replicate 3
"RNAseq service was performed by Sequentia Biotech SL (Barcelona,Spain). RNA concentration in each sample was assayed with a ND-1000 spectrophotometer(NanoDrop) and its quality assessed with the TapeStation 4200 (Agilent Technologies). Indexedlibraries were prepared from 1 g/ea purified RNA with TruSeq Stranded mRNA Sample Prep Kit(Illumina) according to the manufacturers instructions. Libraries were quantified using theTapeStation 4200 and pooled such that each index-tagged sample was present in equimolaramounts, with final concentration of the pooled samples of 2 nM. The pooled samples weresubject to cluster generation and sequencing using a NextSeq 500 System (Illumina) in a 2x75paired end format at a final concentration of 1.8 pmol."