RNA-seq of Arabidopsis pistil: 67h in LL
mRNA was enriched using oligo(dT) beads. rRNA was removed using the Ribo-Zero kit that leaves the mRNA. First, the mRNA was fragmented randomly by adding fragmentation buffer, then the cDNA is synthesized by using mRNA template and random hexamers primer, after which a custom second-strand synthesis buffer (Illumina) , dNTPs, RNase H and DNA polymerase I were added to initiate the second-strand synthesis. Second, the double-stranded cDNA library was completed through size selection and PCR enrichment.