Pacbio raw reads of Hippocampus erectus: male tail muscle
A mature, male lined seahorse from the aquatic farm in Fujian province, China was used as a sample for the de novo genome assembly. Genomic DNA was extracted from tail muscles using a standard phenol/chloroform extraction protocol. Single-Molecule, Real-Time (SMRT) sequencing was performed using a total of 5 g of the genomic DNA to generate a 20 kb library according to the manufacturers instructions (Pacific Biosciences, USA). Subreads were obtained after size selection on a BluePippin system (Sage Science, USA). SMRT genome sequencing was performed on a PacBio Sequel platform (Pacific Biosciences, USA) to an approximate coverage of 113-fold.