greenCUT&RUN against (GFP)-NFYA - replicate 2: control
Purified DNA fragments were subjected to library preparation with NEB Next Ultra II and NEB Multiplex Oligo Set I/II as per manufacturer (New England Biolabs) protocol without size selection. In case of TBP, we followed the library preparation protocol of Skene, et al (2017) to include fragments of short size . For each library, DNA concentration was determined using using a Qubit instrument (Invitrogen, USA) and size distribution was analyzed on Agilent Bioanalyzer (DNA high sensitivity assay) or Agilent 4200TapeStation (D5000 Screentape). Libraries were pooled according to desired molarity with 1% PhiX controls for run on the MiniSeq (Illumina) or without PhiX for runs on the MiSeq (276 paired end, Illumina).