WGS of Staphylococcus aureus
The sequencing libraries were accomplished using Nextera XT DNA Library Preparation Kit (Illumina), according to the instructions of the manufacturer except from the following modifications: half of the recommended quantity of the working volumes and library amplification and clean up were used. KK4835 KAPA Library Quantification Kit qPCR (Roche, Rotkreuz, Switzerland) was performed to quantification and normalization of libraries. The whole genome sequencing was carried out on the Illuminas MiSeq platform and MiSeq v3 Reagent Kit (Illumina)