RNA-seq of clone #15 of Nalm6 cells with additional chromosome 21 (T21)
Total RNA was isolated using TRIzol. RNA concentration was determined using the DeNovix (Wilmington) and Qubit with the Qubit RNA broad range kit (Thermo Fischer Scientific). RNA integrity was analysed using Bioanalyzer and Agilent RNA 6000 nano kit (Agilent Technologies). Subsequently, library preparation and RNA sequencing was performed by Novogene (Hong Kong, China). Library preparation was done with the TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human/Mouse/Rat kit. Paired-end sequencing, of 150 base pair long reads, was done on the NovaSeq6000 (Illumina), generating at least 50 million raw reads per sample.