RNA-seq of cultured SARS-CoV-2
Using 18ul(6ul +12ul H2O) of extracted RNA treated with TURBO DNase and first strand cDNA was synthesized using Superscript IV and random hexamers(Thermo Fisher). Double stranded cDNA was created using Sequenase Version 2.0 and purified using 1.6 volumes of AMpure XP beads (Beckman-Coulter)