Rescue clones from Plate 3
E. coli BW25113 proB760::kan (JW0232-1) was obtained from the Keio collection (Baba et al., 2006). Aliquots of frozen stocks of the proB knockout strain were scraped onto 140 plates containing M9glucose medium [agar (2% w/v), M9 salts (1x), glucose (0.4%, w/v), MgSO4 (2 mM), CaCl2 (100 M)] supplemented with Kanamycin (Kan; 30gmL-1). Plates were incubated at 37C for up to 4 days, and colony formation was monitored.