A complete genome of Psychrobacillus sp. INOP01
Library preparation was performed using the SMRTbell template prep kit 1.0. SPv3 and SMRTbell barcoded adapter complete kit-96 (PacBio), following the Procedure and Checklist Preparing Multiplexed Microbial SMRTbell Libraries for the PacBio Sequel System (PacBio). Briefly, genomic DNA from overnight cell culture grown in standard nutrient broth (15 g/L of peptone, 3 g/L of yeast extract, 6 g/L of sodium chloride, 1 g/L glucose; pH 7.50.2) at 30 C was extracted using the Genomic-tip 20/G kit (Qiagen, Hilden, Germany) as recommended by the manufacturer. Genomic DNA was sheared to approximately 10 kb using g-TUBEs (Covaris Inc., Woburn, MA) and further processed according to the protocol.