gut microbiota sample 2 collected from pond 1 in July, 2020
DNA extraction and high-throughput sequencing of 16S rDNA were performed as previously described. The V4-V5 hypervariable region of prokaryotic 16S rDNA was amplified using primers 515F and 909R. The amplicons were purified, quantified, and sequenced using a HiSeq 3000 platform.