RNA-Seq of Melinaea marsaeus rileyi pupa (run1)
Tissue samples were homogenised in 600 l of RLT buffer with TissueLyser (Qiagen, Hilden, Germany). Total RNA was then extracted according to the manufacturers protocol (RNeasy Mini kit, Qiagen, Hilden, Germany) and eluted in 30 l of RNase-free water. To avoid genomic contamination, RNase-free DNase treatment (Qiagen, Hilden, Germany) was performed during RNA extraction.