16S of Synthetic Community: In Agar
amplified the V3-V4 regions of the bacterial 16S rRNA. PCR conditions were as follows with the Kapa HiFi Hotstart readymix. Triplicate PCR products were pooled and then diluted twice. PCR products were purified using Sera-mag SpeedBeads (Fisher # 09-981-123) and quantified with a Qubit 2.0 fluorometer (Invitrogen) and then diluted to 6 pM for sequencing. Sequencing was performed on an Illumina MiSeq instrument using a 600- cycle V3 chemistry kit with 10% PhiX.