Illumina MiSeq sequencing; qiita_ptid_11217:13917.forehead.34.plateA.wellH01
All volunteers were offered a shower gel free of nanoparticles, alcohol and skin neutral in pH (shower gel ultra sensitive, Balea MED, Karlsruhe, Germany) and agreed to use this instead of their individual products for seven days before sampling. Furthermore, they were instructed not to apply cream or body lotion on sampling areas for seven days and to refrain from showering for at least 24 hours before sampling. The location sampled were forehead, forearm and on the feet the area between all toes. Sampling was performed by the volunteers themselves in the morning and according to a standard operating protocol, using a 18 cm2 template for swabbing forehead and forearm, sterile cotton swabs for each body site, wetted and collected into 5ml sterile 0.9% saline (Fresenius Kabi, Bad Homburg, Germany). All samples were kept at 4C and processed within four hours after collection.