ChIP-seqofArabidopsisthaliana:seedlingH3K4me1_ashh2
Chromatinwasextractedfromwholeseedlingsgrownfor15daysonMSmediaandshearedwithcovarissonicator.Antibodiesusedforimmunoprecipitationare;H3(ab1791;Abcam),H3K4me1(ab8895;Abcam),H3K4me2(ab32356;Abcam),H3K4me3(ab8580;Abcam),H3K36me2(MABI0332;MBL),H3K36me3(MABI0333;MBL),RNAP2totalCTD(CMA601;MBL),RNAP2phosphoS2(CMA602;MBL),RNAP2phosphoS5(CMA603;MBL),FLAG(F1804;SIGMA).LibrariesweremadewithKAPAHyperPrepKit(KapaBiosystems),anddualsize-selectedusingAgencourtAMPureXP(BeckmanCoulter)toenrich200500bpfragments.Thelibrarieswere50 bpsingle-endsequencedbytheHiSeq4000sequencer.