MiSeq of soil fungal communities: herbivore-adapted savanna ecosystem
Partial ITS3-ITS4 of the ITS2 region libraries, were conducted by following the protocol described in the metagenomics sequencing Library guide (Illumina, CA, USA). Briefly, the library preparation protocol entailed a first amplicon PCR step, followed by amplicon purification, and then the addition of barcodes. Sequences were demultiplexed and depleted of Illumina indexes and primers.