RNA-seq of Aedes aegypti: 1-day midgut
Total RNA was extracted from each sample using a Trizol kit (Qiagen). RNA integrity was checked using an Agilent 2100 Bioanalyzer (Agilent Technologies). Ten mg RNA from each sample was used to isolate mRNA through oligo (dT) magnetic beads (Invitrogen). The 2100 bp paired-end RNA-seq libraries were prepared according to the manufacturers protocol and sequenced using the Illumina HiSeq 2020 platform (Illumina).