16S rRNA amplicon sequencing of marine sediment Prokaryotic communities from the Gulf of Gdansk: MET1-MP sample 90-95 cm bsf
Metagenomic analysis of bacterial and archaeal populations was performed by Genomed (Warsaw, Poland), based on the hypervariable V3-V4 region of the 16S rRNA gene. Specific sequences of 341F and 785R primers were used to amplify the selected region and prepare the library. The PCR reaction was carried out using Q5 Hot Start High- Fidelity 2X Master Mix. Sequencing took place on a MiSeq sequencer, using paired-end (PE) technology, 2x250nt,with an Illumina v2 kit. Automatic preliminary data analysis was performed on a MiSeq device using MiSeq Reporter (MSR) v2.6 software. The analysis consisted of two stages: 1) automatic demultiplexing of samples, 2) generation of fastq files containing raw readings.