PoolSeq of Cichlidogyrus casuarinus
In total, 80 individuals of C. casuarinus ex H. stenosoma were pooled for mitogenome assembly and to compare their level of variation in the cox1 region with that based on Sanger sequencing of individual amplicons. Genomic DNA of the pooled samples was extracted using the Quick-DNATM Miniprep Plus Kit (Zymo Research) following the manufacturer's instructions with minor modifications, initial incubation overnight, and elution in 2x50 ul after 10 minutes incubation at RT each. Library preparation (Illumina TruSeq Nano, 550 bp insert size) and sequencing on NovaSeq6000 (2x 150bp)