M_TR_Rep1
Total RNA from standard TRIzol isolation was transcribed by NEBNext Ultra Directional RNA Library Prep Kit. The filtering process was done as followed: (1) Discard reads with adaptor contamination. (2) Discard reads when uncertain nucleotides constitute more than 10% of either read (N > 10%). (3) Discard reads when low quality nucleotides (base quality less than 20) constitute more than 50% of the read. Mapping to reference genome was done with HISAT2 (V2.0.5) by default parameter. Quantification was done with HTSeq (v0.6.1) and -m union as parameters.