Illumina sequencing of Treponema pallidum tprK amplicon
The region of interest was amplified using a HiFi polymerase (Takara). The resulting PCR product was purified with 0.6x volumes of Ampure XP Beads (Beckman Coulter). Library was prepared using the Nextera kit, cleaned with 0.6x volumes Ampure XP beads and sequenced on a 1x192 bp MiSeq run.