H-CK-2
To find key genes accounting for the opeing of wheat florets after low light stress, spikes at young microspore stage and grain filling stages were collected for RNA-seq. Total RNA was extracted using Plant RNA Purification Reagent (Invitrogen). The cDNA libraries were constructed using TruseqTM RNA sample prep Kit (Illumina) according to the manufactuers instructions. The sequencing was performed by Shanghai Majorbio Bio-pharm Technology Company using an Illumina NovaSeq 6000.