RNA-Seq of Low sulphur treated Ri-0
Total RNA from ten-day-old seedlings was isolated and quantified using Spectrum Plant Total RNA Kit (Sigma-Aldrich, USA) and spectrophotometer (ND-1000 NanoDrop, Wilmington, DE), respectively. RNase-free-DNase-I (Fermentas, USA) treatment was given to remove genomic DNA contamination from RNA samples. The raw data of the transcriptome datasets were generated on NextSeq 500 and raw reads generated were filtered using Trimmomatic (v 0.35) with quality value QV>20 and other contaminants. The Illumina NextSeq transcriptome data for all samples were mapped on the reference genome of A. thaliana TAIR10 with a genome size of 119 Mb using bwa mem with default parameters.