ITS sequencing of soils under tillage and residue management in Akron, CO
Soil DNA was extracted from 0.25 g of 2mm sieved soil samples.The DNA quality and quantity were determined by using a NanoDrop Spectrophotometer. Sequencing libraries were constructed by the amplification of the ITS 2 region (ITS1f/ITS2 primers). To generate the DNA libraries for subsequent sequencing, we amplified each sample in duplicate in 25 l PCR mixtures using a one-step PCR reaction with individually barcoded Illumina adapters ligated to the relevant primers. All sample libraries were pooled, cleaned, and normalized using the ThermoFisher Scientific SequalPrep normalization plate kit.