Drosophila subpulchrella genome sequencing using illumina
We extracted DNA from adult females following the protocol of Chakraborty et al. 2016. (Nucleic Acids Res. 44, 147.) The DNA was sheared using 20 plunges of 21-gauge needle and size selected using the 30kb lower cutoff size on Blue Pippin size selection system (Sage Science). 30kb SMRTbell template library was prepared from the size selected DNA and was sequenced on 4 SMRTcells of Pacific Biosciences Sequel I platform.