RNA from Lithobius forficatus front legs (1-5)
Total RNA was extracted using standard TRIzol protocol (ThermoFischer, USA). RNA samples were submitted to the Molecular Biosciences Sequencing Facility at the University of Queensland for library preparation and sequencing. Paired end libraries with 180 bp insert size was constructed using the Illumina TruSeq-3 Stranded mRNA kit and sequenced on an Illumina NextSeq using a 300 cycle (2x150bp) Mid Output Run.