Comprehensive Characterization of Cancer Driver LncRNAs in Nasopharyngeal Carcinoma
Total RNA from the exosomes was used for library preparation and sequencing, which were performed at RiboBio (Guangzhou, China). Briefly, RNA was fragmented to approximately 200 bp. Subsequently, the collected RNAs were subjected to first strand and second strand cDNA synthesis followed by adaptor ligation and low-cycle PCR enrichment according to the instructions provided with the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA). The purified library products were evaluated using the Agilent 2200 TapeStation and Qubit2.0 (Life Technologies, USA) and then sequenced (2 150 bp) using a HiSeq30000