CD34+ Healthy donor: Sample SSF2062.G05
Fresh bone marrow mononuclear cells were stained with alexa fluor 488-anti-CD34 (Biolegend, San 28 Diego, CA 343518, 1:20) and single CD34+ cells were sorted into 96 well plates using a FACS Aria II 17 instrument (BD, Franklin Lakes, NJ, USA). cDNA libraries were prepared using the SMART-Seq v4 Ultra 20 Low RNA Kit (Clontech). Libraries were sequenced on a HiSeq 2500 Instrument 21 (Illumina, San Diego, CA) to a read depth of ~3 M paired-end, 25 bp reads per single cell, or ~12 22 M paired-end, 25 bp reads per 100 cells. Paired-end reads were mapped to the hg38 human 35 transcriptome (Gencode v24) using STAR v2.4.2a. PMCID: PMC6715510