Metagenome of marine water: bacterial fraction
DNA was extracted using the Diatom DNA prep kit from the bacterial fraction of marine water (3L) sattled on 0.22 m Sterivex filter. DNA was then extracted twice with phenol-chloroform and chloroform, and precipitated in ethanol for the additional purification. NGS libraries were made using NEBNext Ultra II DNA Library Prep kit (NEB).