Embryonic mandibles of mice at E14.5
The total RNA concentration and its integrity were evaluated by Agilent 2100 Bioanalyzer (Agilent, CA, USA). A library was independently prepared using 1 g of total RNA for each sample using the Illumina TruSeq RNA Sample Prep Kit v2 (Illumina, Inc., San Diego, CA, USA) following the manufacturers instructions (Part# 15026495 Rev. F). The paired end sequencing was performed by Macrogen Incorporated on the Illumina HiSeq 4000 platform (Illumina, Inc., San Diego, CA, USA).