16S Sequencing of mock community DNA mixed bacterial population
Sequencing of the V4 region of the 16S rRNA gene sequences on the MiSeq platform was evaluated on a mock bacterial community. The 19 organisms in the mock community are as follows: Campylobacter jejuni subsp. jejuni NCTC 11168, Bacteroides thetaiotaomicron VPI-5482, Lactobacillus delbrueckii subspecies bulgaricus ATCC 11842, Parabacteroides merdae strain JCM9497,  Methanobrevibacter smithii DSM 2375, Faecalibacterium prausnitzii A2-165, Escherichia coli strain K-12 substrain MG1655, Oxalobacter formigenes HOxBLS, Steptococcus parasanguinis ATCC 15912, Coriobacterium glomerans PW2, Megasphaera elsdenii DSM 20460, Roseburia hominis A2-183, Brachyspira hyodysenteriae ATCC 27164, Bordetella bronchiseptica 1289, Haemophilus parasuis 29755, Staphylococcus aureus USA300_TCH1516, Salmonella enterica subsp. enterica serovar Typhimurium, Oscillibacter valericigenes Sjm 18-20, Desulfovibrio gigas DSM 1382