Treponema denticola SP7
Sequencing library was prepared by BGI (HK) as described below: genomic DNA was extracted and fragmented by Covaris S/E210. Then the overhangs resulting from fragmentation are converted into blunt ends by using T4 DNA polymerase, Klenow Fragment and T4 Polynucleotide Kinase. After adding an A base to the 3' end of the blunt phosphorylated DNA fragments, adapters are ligated to the ends of the DNA fragments. The desired fragments at 150bp were purified though gel-electrophoresis, then selectively enriched and amplified by PCR. The index tag were introduced into the adapter at the PCR stage, followed by ibrary quality test. The qualified BS library was used for sequencing.