RAD-seq of Euptelea pleiosperma
The qualified genomic DNA samples were mixed in equal amount, treated with restriction enzymes, and P1 adaptors were added (the adaptors contain amplification primer sites, Illumina sequencing primers binding sites sequences, and short tag sequences to distinguish different samples). The DNA sample was interrupted into short sequences of 300-700bp in length and added with P2 adaptors (P2 adaptors include the reverse complementary amplification primer sites), and then RAD tags were enriched by PCR amplification.