CD34+ Shwachman-Diamond Syndrome: Sample SCC10035.H01
CD34+ cells were positively selected from fresh bone marrow mononuclear cells on an AutoMACS instrument using the Indirect CD34 MicroBead Kit (Miltenyi, Bergisch Gladbach, Germany), and singulated on the C1 Instrument (Fluidigm, San Francisco, CA, USA). cDNA libraries were prepared using the SMARTer Ultra Low RNA Kit (Clontech, Mountain View, CA, 11 USA). Libraries were sequenced on a HiSeq 2500 Instrument 21 (Illumina, San Diego, CA) to a read depth of ~3 M paired-end, 25 bp reads per single cell, or ~12 22 M paired-end, 25 bp reads per 100 cells. Paired-end reads were mapped to the hg38 human 35 transcriptome (Gencode v24) using STAR v2.4.2a. PMCID: PMC6715635