Montipora capitata associated prokaryotic 16S rRNA (V3V4 multivariable region) sequencing: MC-206_T5-HiT_1744
Cells in each subsample were lysed using liquid nitrogen and mechanical grinding. Total DNA was isolated using a Qiagen AllPrep DNA/RNA/miRNA Universal Kit and 16S-rRNA amplicon sequencing libraries were prepared using primers designed for the V3 and V4 hypervariable region. Quality control was performed using a Qubit fluorometer and an Agilent Bioanalyer, with the target library length being ~600 base pairs. Libraries were sequenced by Genewiz on an Illumina MiSeq (2x300 bp).