Whole-genome sequencing of Nannochloris desiccata UTEX 2526
Cells were washed and embedded into 1% LMP agarose plugs. The plugs were incubated in a protoplasting solution to remove the cell wall followed by lysis with proteinase K.  The agarose plugs were digested to release the gDNA into the solution from which it was subsequently purified using High salt: Phenol:Chloroform:IsoAmyl alcohol protocol from PacBio and concentrated with AMPure PB beads. gDNA was fragmented using the Megaruptor 2 instrument with a target size of 20kb. SMRTbell low input HiFi libraries were constructed according to the PacBio Express low input HiFi template Prep protocol. The libraries were sequenced on PacBio Sequel instrument using reagent chemistry v.3.0 and DNA polymerase v.3.0 Two SMRT cells 1M LR were used per library. 20-hr movies were recorded.