Shotgun seqeuncing of bison semen
Genomic DNA was extracted from the semen samples using a standardphenol:chloroform method as previously described in Heaton et al. (2001). DNA was thensheared using sonication on a Covaris S220 instrument (Covaris Inc., Woburn, MA) to generatefragments less than 800 base pairs in length. Indexed libraries were constructed from thesheared DNA using the Tru-Seq PCR-Free Kit (Illumina Inc., San Diego CA) and weresequenced on an Illumina NextSeq500 instrument using a 2x150 cycle paired end kit.