RNA-seq of B73 and GY, shrunken micro-endosperm pool
To identify the genes controlling the micro-endosperm trait, three  different F2 populations were generated by crossing the micro-endosperm high  oil inbred line GY with other three normal corn inbred lines, Jing724, B73  and Jing92,respectively,followed by selfing.F2 seeds produced within each ear  of F1 plants can be divided into 3 classes, including wild type(WT) normal  seeds, translucent micro-endosperm seeds and shrunken micro-endosperm seeds  and the ratio of them is 9:4:3, indicating that the micro-endosperm trait was  controlled by two independent recessive genes and the translucent  micro-endosperm gene was epistatic to the shrunken micro-endosperm gene. The  RNAs of the shrunken micro-endosperm pool and the normal endosperm pool from  B73/GY F2 population were used for BSR-seq analysis.