RNA-seq of Jing92 and GY, the mixed pool of normal and shrunken  micro-endosperm seedlings
To identify the genes controlling the micro-endosperm trait, three  different F2 populations were generated by crossing the micro-endosperm high  oil inbred line GY with other three normal corn inbred lines, Jing724, B73,  and Jing92, respectively,followed by selfing.F2 seeds produced within each  ear of F1 plants can be divided into 3 classes, including wild type(WT)  normal seeds, translucent micro-endosperm seeds and shrunken micro-endosperm  seeds and the ratio of them is 9:4:3, indicating that the micro-endosperm  trait was controlled by two independent recessive genes and the translucent  micro-endosperm gene was epistatic to the shrunken micro-endosperm gene.  Total RNAs of shrunken micro-endosperm pool and the mixed pool (normal and  shrunken micro-endosperm seedlings) from each of three independent F2  populations were used to preform BSR-seq.