RNA-Seq of mus musculus adult cecum
The cecum was homogenized and total RNA was isolated by a TRIzol Plus kit (Life Technologies) according to the manufacturers method including an on-the-column DNase digestion. The quality of the isolated RNA was assessed using an Agilent 2100 Bioanalyzer instrument (Agilent Technologies, Santa Clara, CA) and RNA 6000 Nano LabChip assay.  mRNA libraries were produced using the KAPA straded mRNA-Seq kit. The samples were pair end sequenced on a NovaSeq 6000 S4 (Illumina San Diego, CA) producing paired 150 bp reads at a depth of ~40 M read pairs per sample.