Metagenomic analysis of Microcystis aeruginosa NIBR18 using Nanopore sequencing
Twenty ng genomic DNA was cut into 10 kb using a g-tube (Covaris, U.K.). DNA samples were repaired using NEBNext FFPE DNA repair Mix and NEBNext Ultra II End repair/dA-tailing module reagents (SQK-LSK109). After the DNA repair step, adapter ligation and clean-up were performed using a ligation sequencing kit. A total of 452 ng DNA was used by sequencing. Sequencing was obtained with passed read counts of 7.01 Gb using a flow cell on the MinION portable sequencer.