p17 plasmid sequencing
Whole-genome sequencing was performed (Omega Bioservices). DNA concentration was measured using the QuantiFluor dsDNA System on a Quantus Fluorometer (Promega). A Kapa Biosystems HyperPrep kit (Kapa Biosystems) was used for whole-genome library construction. Briefly, 1 mg of genomic DNA was fragmented using a Bioruptor sonicator (Diagenode). DNA fragment ends were repaired, 3 adenylated, and ligated to adapters. The resulting adapter-ligated libraries were PCR-amplified, Illumina indexes added, and pooled for multiplexed sequencing on an Illumina Miseq platform (Illumina) using the pair-end 300 bp run format.