Single-cell BCR repetoire requencing of homo sapiens:  adult
B cells were isolated from PBMCs by EasySep Human Pan-B cell Enrichment Kit (immunomagnetic negative selection kit, STEMCELL Technologies) per the manufacturers instructions. Additional fluorescence-activated cell sorting was then performed using a FACSAria flow cytometer (Becton Dickinson, Mountain View, California, USA) and visualized using the python package FlowCytometryTools v0.5.0; B cells labeled with 7AAD Viability Staining Solution (BioLegend) and FITC-labeled anti-IgD antibody (mouse anti-human monoclonal antibody, BioLegend clone IA6-2) were removed to exclude nonviable cells and all naive B cell populations. Sorted B cells were then loaded into the Chromium Controller (10x Genomics). Single-cell gene expression libraries were prepared using the Chromium Single-cell 5 Reagent Kit (10x Genomics) for version 1 chemistry per the manufacturers protocol.