16s rRNA sequencing of mus musculus: fecal pellet
The V4 region of the 16S rRNA gene was amplified using the LongAmp Taq  PCR Kit (New England Biolabs, Ipswich, MA, USA) and using primers designed by  Caporaso and colleagues. The 16S rRNA gene libraries were quantitated using  Pico Green and adjusted to a concentration of 4 nM. Sequencing was performed  at the University of Alabama Heflin Center for Genomic Sciences on an  Illumina MiSeq instrument.