Traditom GBS of Solanum lycopersicum: TRBA1320
Genomic DNA was isolated from young leaves of 5-10 seedlings per accession, using the DNEASY 96 Plant Mini Kit (Qiagen, Germany). The library strategy was Genotype-By-Sequencing (GBS), and was performed following the procedure reported by Elshire (2011). Briefly, DNA was digested with the restriction enzyme ApeK I, barcoded libraries were prepared to track each accession and the DNA sequence corresponding to the region flanking the ApeK I site was obtained on an Illumina HiSeq 2000