RNA-Seq of Harpalus pensylvanicus, pooled adults, pygidial gland secretory lobes
Total RNA extraction (TRIzol), column purification (Directzol MicroPrep Kit), poly-A pulldown + Illumina Nextera library prep, sequenced 150 bp paired-end on an Illumina NovaSeq 6000 at a target depth of 50M reads/library