Ridgeia piscesae whole genome sequence mate pair 500 bp insert size 2
Ridgeia piscesae were obtained from the deep-sea hydrothermal vent areas at the Juan de Fuca Ridge. After removing the parasitic microbe enrichment part, genomic DNA (gDNA) was isolated from muscle using a standard phenol/chloroform extraction protocol and broken into random fragments for whole-genome shotgun (WGS) sequencing. Short-insert (180 bp, 300 bp and 500 bp) paired-end libraries were prepared using the Next Ultra DNA Library Prep Kit for Illumina (NEB, USA) according to the standard protocols, respectively. Large-insert (2 kb, 5 kb, 10 kb and 15 kb) mate-pair libraries were prepared following the Cre-lox recombination-based protocol. All DNA libraries were subjected to paired-end 100 bp sequencing on the Illumina Hiseq 2000 platform.