Bisulfite RNA seq of 6 months KO mouse:ovary
mRNA was separated from 12.5 g of total RNA and bisulfite converted using the EZ RNA methylation kit with high-stringency conditions. Then, RNA libraries were constructed with the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs, United States). An Agilent 2100 was employed to evaluate the library quality, and then the library was sequenced with Illumina NovaSeq.