RNA-seq of Botrytis cinerea: wild type replicate 2
RNA was obtained from mycelium of wild type B05.10 replicate 2 grown in Czapek Dox +1% sucrose liquid medium on an orbital shaker with agitation at 150 rpm for 7 hours at 21°C. RNA isolation was performed with the RNAprep Pure Plant Kit (TianGen, China). Sequencing library was generated using NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA). After a series of processings, the library preparations were sequenced on an Illumina platform and paired-end reads were generated.