BGISEQ-500 RNA-Seq of Photuris sp. adult female, body minus lantern containing abdominal segments
The body of an adult female Photuris sp. "Photuris 3", raised from a larva in the laboratory, was separated from the lantern containing abdominal segments using a razor blade. Total RNA was extracted using the Qiagen Lipid Tissue kit (Trizol w/ silica column cleanup). Extracted RNA was provided to Beijing Genomics Institute (Beijing, China) for gratis RNA-Seq library prep and sequencing as an evaluation of their BGISEQ-500 platform. The library was sequenced on two physical flowcell lanes. Fastq files from lanes L01 & L02 are uploaded as 4 separate files to this SRA ID. Multiplexed on the same lanes & potentially contaminating this dataset from low level index swapping were RNA-Seq samples from Ellychnia corrusca, Ignelater luminosus, a Rubiaceae family plant, a Euphorbiaceae family plant, a Rhamnaceae family plant, a Piperaceae family plant, and a Strophariaceae family mushroom.