Traditional Dahi Metagenome DNA kaithal
Adapter-ligated DNA was purified using JetSeq clean up beads. Resultant fragments were amplified for 4 cycles of PCR using Illumina-compatible primers provided in the NEXTflex Rapid DNA sequencing Bundle.Final PCR products were purified with JetSeq cleanup beads followed by library quality control. Sequencing libraries were quantified by Qubit fluorometer and the fragment size distributions were analyzed on Agilent 2200 TapeStation.