assemble of genome of Alteromonas macleodii filamentous phage phiAFP1
For Illumina pair-end sequencing of each strain, at least 1g genomic DNA was used for sequencinglibrary construction. Paired-end libraries with insert sizes of ~400bp were prepared followingIlluminas standard genomic DNA library preparation procedure.Purified genomic DNA is sheared into smaller fragments with a desired size by Covaris, and blunt ends are generated by using T4 DNApolymerase. After adding an A base to the 3' end of the blunt phosphorylated DNA fragments,adapters are ligated to the ends of the DNA fragments. The desired fragments can be purified through gel-electrophoresis, then selectively enriched and amplified by PCR.