Fecal metagenome of pigs weaned at different ages
Metagenomic libraries were prepared using 700 ng of DNA and the TruSeq DNA PCR-Free Library Prep Kit (Illumina Inc.) following the manufacturers recommended protocol. Briefly, DNA was fragmented to an average length of 400 bp with a Covaris LE220 instrument, end-repaired, A-tailed, and indexed with TruSeq Illumina adapters. Libraries were then validated for size and the abon a Fragment Analyzer system with a High Sensitivity NGS Fragment Kit (Agilent Technologies, Mississauga, ON, Canada) to check for size and quantified by qPCR using the Kapa Library Quantification Illumina/ABI Prism Kit protocol (KAPA Biosystems, Wilmington, MA, USA). Equimolar quantities of each library were then pooled and sequenced on the Illumina Novaseq 6000 instrument with a SP flowcell (2 x 250 bp) following manufacturers instructions.