WGS of BTV8/LUX/2020/1 isolate
Total RNA was extracted using a NucleoSpin RNA virus kit (Macherey-Nagel) and treated with Baseline-ZERO DNase (Lucigen) and Mung Bean nuclease (New England Biolabs). cDNA was synthesized with SuperScript IV Reverse Transcriptase (Thermo Fisher Scientific) using a mixture of random hexamers and primers targeting the conserved BTV termini (7). Nextera XT sequencing libraries were prepared and analysed on a MiSeq System using a MiSeq Reagent Kit V3 (2x300 bp, Illumina), yielding approximately 2.5 million read pairs per sample.