Method evaluation of DNA extraction method HQ: caecum 3 microbiome
Caecum sample was homogenized in PBS, aliquote was extacted with method HQ. NanoDrop values were determined and 16S PCR was performed in triplicate for the extracted DNA present in the caecum microbiome. 16S amplicons were purified, pooled and quantity and quality were measured with NanoDrop One and Quibit, respectively. 16S metagenomic sequence library was generated with Illumina MiSeq platform.