DNA-Seq of microflora
The archaea-specific primer pairs Arch519F/Arch915R, the fungi-specific primer pairs ITS1f/ITS2R were used to amplify the archaeal 16S rRNA gene and fungal ITS gene, respectively. The PCR reaction was performed as follows: 95  for 5 min, 27 cycles at 95  for 30 s, 55  for 30 s, 72  for 45 s, and finally 72  for 10 min. Sequencing was performed using an Illumina Miseq platform.