Genomic data of Populus tremuloides
Libraries for Illumina triple digest GbS (3D-GbS or 3D-RAD) were prepared at the Plateforme dAnalyses Gnomiques of the Institut de Biologie Intgrative et des Systmes (IBIS, Universit Laval, Qubec, Canada) using Polands procedure (Poland et al., 2012) with the following modifications to maximize SNP numbers: (i) a restriction enzyme (NsiI) was added to the combination (PstI/MspI) described in Poland et al (2012) (ii) a size selection (150-400 bp) step was performed using a blue Pippin (SAGE sciences) with elution times set between 50 and 65 min on  a 2% gel prior to final library amplification. Plate barcodes were added according to the procedure described in Colston-Nepali et al., 2019.