RNAseq of rose peduncles undergoing bent neck
Bunches of H30 stems were held in tap water with rose flower food (Chrysal) to replicate consumer conditions. 2 cm peduncle sections from straight peduncles or peduncles that were bent to <90 or >90  degrees to vertical were excised using a sterile razor blade and flash frozen in liquid nitrogen. Mid-point in necking was ~20 mm below the flower head therefore 2 cm samples were taken starting 1cm below the flower head.Three peduncle sections were ground to produce one pooled sample d under liquid nitrogen and RNA extracted as descrbed in Moazzam Jazi et al (2015)  https://doi:10.1007/s12298-015-0319-x. All RNA samples were quality tested using a Qubit fluorometer and then sequenced using an Illumina NextSeq 500 to produce paired-end reads for each sample.