WGS_MRSA_invasive from children
Genomic sequencing of the isolates was carried out by MiSeq Sequencing System (Illumina), BCL files generated were converted to FASTQ format using BCLtoFASTQ v2.19 tool installed in TeideHPC (Spain). Simultaneously, demultiplexing was carried out with the same tool, obtaining the corresponding files to R1 and R2 sequencing reads. The reads were subjected to a trimming process to improve their quality using the Trimmomatic v0.38.1 software. Subsequently, de novo assembly was carried out with Unicycler v0.4.8.0 and its quality control with Quast Genome assembly Quality v5.0.2 to certify the quality of the reads.