Metagenomics of clinical sample: VCS negative control
Samples were collected from naso/oropharyngeal swab. Libraries were prepared in duplicate using KAPA HyperPrep Kit following the instructions provided by the manufacturer (Roche-Kapa Biosystems). Briefly, dsDNA was enzymatically fragmented, indexed with Dual Indexes, amplified and the quality of the fragments and the concentration measured by Qubit 3.0 Fluorometer (Thermo Fisher Scientific). Then, one replicate of the libraries was hybridized with probes designed to capture sequences from vertebrate viral pathogens (VirCapSeq Enrichment Kit, Roche). Two negative controls were also processed, one of them also processed with the enrichment kit. Sequencing was performed on an Illumina MiSeq (2x300bp).