RNA-Seq chinese sturgeon primary macrophages treated with 100 ng/mL interferon gama in PBS repetition 3
After DNA digestion using DNase, the mRNA was enriched using Oligo(dT) magnetic beads. The fragmentation buffer was added to break the mRNA into short fragments. Using the fragmented mRNA as a template, first strand cDNA was synthesized using reverse transcription PCR and random six-base primers, followed by a second strand synthesis reaction to produce double-stranded cDNA, which was purified using PCR cleanup and gel extraction kits (Takara). After end repair and the addition of poly(A), the short fragments were linked with sequencing adapters, PCR amplification was performed come after with the fragment size was selected