mRNA-Seq of Sumai3:spiklets and rachics
Oligo(dT)-attached magnetic beads were used to purify mRNA.The cDNA fragments obtained from previous step were amplified by PCR,and the products were purified by Ampure XP Beads,The double stranded PCR products from the previous step were heated to be denatured and circularized by the splint oligo sequence to get the final library.