StP2_Bowman_3
Developing barley inflorescence tissues were enriched for double ridge, stamen primordium and awn primordium stages (W2.0, W3.5 and 5.0, respectively) and frozen in liquid nitrogen. Total mRNA was extracted using TRIzol reagent (Invitrogen). Contaminated genomic DNA was removed using a DNA-free kit (Ambion). The quality of the total mRNA was evaluated using a bio analyzer (Agilent). RNA libraries were prepared for sequencing using standard Illumina protocols at the MPIPZ genome center.