RNASeq of human adult lung tissues_control8
Total RNA was isolated from formalin-fixed paraffin-embedded (FFPE) lung or colon tissue from cases and controls using the RNeasy FFPE kit (Qiagen) according to the manufacturers instructions with a minor modification. Thin tissue sections weighing ~0.1 mg were used for each sample, and an additional genomic DNA removal step was performed by treating the sample with 2 units of TURBO DNA-free DNase (Invitrogen) for 30 min at 37 . The Trio RNA-Seq Library Preparation Kit (Nugen; Redwood City, CA) was used to generate sequencing libraries as per the manufacturers instructions, with 15 ng RNA used as input. The library size and quality were assessed using an Agilent Bioanalyzer 2100. Pooled libraries were sequenced on an Illumina NextSeq 550 using a 75-cycle kit with single-end read mode.