3G-ESP-WLE18-SC45_50_16S
Samples were collected using a MBARI 3G-ESP attached to a LR-AUV. Extracted with Qiagen DNEasy Blood and Tissue kit. Amplified with NEXTFLEX 16S V4 Amplicon-Seq Kit 2.0 (custom to include Parada/Apprill modifications); full-length primers including sequencing primer are: FWD:GACGCTCTTCCGATCTTATGGTAATTGTGTGYCAGCMGCCGCGGTAA; REV:TGTGCTCTTCCGATCTAGTCAGTCAGCCGGACTACNVGGGTWTCTAAT.