Population genomics of Sporothrix
From each isolate, we extracted 1 g of DNA, as described above. Next, we prepared paired-end libraries using the NEBNext Ultra DNA Library Prep Kit for Illumina. The libraries were quantified using the NEBNext Library Quant Kit for Illumina (New England Biolabs). We pooled equimolar amounts of each library together and sequenced them using  Illumina techology