16s rRNA sequencing of mus musculus: fecal pellet
The V4 region of the 16S rRNA gene was  amplified using the LongAmp Taq PCR Kit (New England Biolabs, Ipswich, MA,  USA) and using primers designed by Caporaso and colleagues. The 16S rRNA gene  libraries were quantitated using Pico Green and adjusted to a concentration  of 4 nM. Sequencing was performed at the University of Alabama Heflin Center  for Genomic Sciences on an Illumina MiSeq instrument.