RNAseq of Tetrahymena thermophila: SB210
RNA was prepared from pelleted cells using Trizol extraction (Invitrogen), DNAse I (NEB, M0303) treatment, phenol:chloroform:isoamyl alcohol (25:24:1) extraction, and ethanol precipitation, followed by resuspension in 10 mM Tris, pH 7.5. Two mg of RNA from each sample was submitted for poly(A)+ library construction using the Illumina TruSeq stranded RNA preparation kit and sequencing on a 75 bp SE NextSeq 500 by the CGB Genomics Service Facility at Indiana University, Bloomington, IN.