PacBio HiFi Sequencing of Cichorium intybus : leaf
High-qualitygenomicDNAwasextractedfromleaftissue,shearedinto10-15kbfragmentsbyCovarisg-TUBEs,enrichedandpurifiedbyAMPurePBmagneticbeads,andtransformedintoSMRTlibrariesbySMRTbellTMExpressTemplatePrepKit2.0andSMRTbellEnzymeCleanupKit,andfinallyrecycledandcheckedbyBluePippinandAgilent2100Bioanalyzer.