16S rRNA- Seq of Gut Microbiota: Parkinson's Disease
Samples were then loaded into an Illumina MiSeq v3 600 cycles cartridge, the run was performed in paired end mode with 275 bp read length for each of forward (Read 1) and reverse (Read 2) reads. After the completion of sequencing run, the data was demultiplexed using bcl2fastq software v2.20 and Fast Q files were generated based on the unique dual barcode sequences.