bacterial communities in bisphenol-contaminated water subjected to bioaugmentation
Amplicon libraries were constructed for the MiSeq platform using bacterial primers 341F (5-CCTACGGGNGGCWGCAG-3) and 785R (5-GACTACHVGGGTATCTAATCC-3) for the V3-V4 region of the 16S rRNA gene. Each library was prepared using Q5 Hotstart High-Fidelity DNA Polymerase (NEBNext  New England BioLabs, Ipswich, MA, USA) according to the manufacturer's instructions.