DNA-Seq of Homo sapients: cfDNA of CCA patient
60 targeted genes sequencing was performed in cfDNA of CCA patient. Sequencing libraries were generated from a total of 40 ng of DNA sample using Agilent SureSelectXT-HS according to the manufacturers protocol. Molecular barcode (MBC) was tagged to DNA fragment results in a unique identifier and indexed DNA libraries. The sequencing libraries were sequenced with 150 bp paired-end reads on Illumina Novaseq 6000 S4.