Lectin-24A gDNA of Drosophila melanogaster
PCR was performed on the gDNA for one of the biological replicates and cDNA from both biological replicates targeting the Lectin-24A gene region on chromosome 2L between 3,717,069 and 3,717,568 (dm6). Lectin-24A sequences obtained from Flybase (FB2020_03) were used to design primers TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGACAAATGGAATACTTGTACGGAAAT (forward) and GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACGCCCAGGAAGTATGATT (reverse) that include adapter overhang sequences.