RNAseq of uninfected salivary glands of adult unfed female Amblyomma maculatum - sample 3
Total RNA was treated with the Ambion GLOBINclear-Human Kit to deplete globin mRNA that may have resulted from the blood meal; the Clontech SmartPCR cDNA kit (Clontech Laboratories, Inc, Mountain View, CA USA, Cat#634926) was used to generate 12 g of cDNA from 100 ng of total RNA. Restriction digestion was used to remove adaptor sequences and the resulting cDNA was fragmented using Covaris (Covaris, Inc., Woburn, MA USA), profiled using Agilent Bioanalyzer, and subjected to Illumina library preparation using TrueSeq RNA prep library (Illumina USA). The libraries were then submitted for Illumina NextSeq500.