RNA-seq of upper aerodigestive tract papilloma: Sample PPRE11487
500 nanograms of total RNA was oligo dT/template-switching oligo (TSO) reverse transcribed using Maxima H Minus reverse transcriptase (Thermo Fisher Scientific). Full-length 2nd strand cDNA was generated by LongAmp Master Mix (New England BioLabs). Nextera-tagmented libraries (Illumina) were generated and equimolar pools were sequenced. All kits were used per manufacturer recommendations.