Dovetail Omni-C sequencing data from Pisaster brevispinus: tube foot from individual M0D059179O
We performed proximity ligation using the DovetailTM Omni-CTM Kit according to the manufacturers protocol with slight modifications. First, we thoroughly ground the specimen tissue with a mortar and pestle in liquid nitrogen. Subsequently, chromatin was fixed in place in the nucleus. We passed the suspended chromatin solution through 100 m and 40 m cell strainers to remove large debris. We digested fixed chromatin under various conditions of DNase I until a suitable fragment length distribution of DNA molecules was obtained. We repaired and ligated the chromatin ends to a biotinylated bridge adapter followed by proximity ligation of adapter containing ends. After proximity ligation, crosslinks were reversed and the DNA purified from proteins. We treated the purified DNA to remove biotin that was not internal to ligated fragments. We generated a library with an Illumina compatible y-adaptor using the NEB Ultra II DNA Library Prep kit (New England Biolabs)  and captured biotin-containing fragments using streptavidin beads. We split the post-capture product into two replicates prior to PCR enrichment to preserve library complexity with each replicate receiving unique dual indices. The Omni-C library was sequenced on an Illumina NovaSeq platform to generate approximately 100 million 2 x 150 bp read pairs per GB genome size.