Unfractionated Iso-seq of Emiliania huxleyi AWI1516 - calcified cells 6h in light phase - Std cDNA protocol - SMRT cell 2
cDNA is synthesized with the SMARTer PCR cDNA synthesis kit (Clontech) then reamplified by PCR using the KAPA HiFi PCR kit (Kapa Biosystems). SMRT bell libraries were constructed with the Pacific Biosciences SMRTbell Template Prep Kit 1.0 and sequenced with Sequel DNA polymerase, binding kit version 2.1 and sequencing chemistry version 2.1 for 10 h on the Sequel.