Size-fractionated Iso-seq of Emiliania huxleyi AWI1516 - calcified cells 6h in light phase - Std cDNA protocol - size fraction 3000-5000bp - SMRT cell 4
Using the SageELF Size Selection System" including cDNA synthesis with the SMARTer PCR cDNA Synthesis kit (Clontech) and amplification by the KAPA HIFI PCR Kit (Kapa Biosystems). The cDNAs were size-selected on SAGEelf (Sage Sciences) into 7 fractions, then the subfractions were reamplified by PCR according to the protocol. PacBio libraries were prepared with the SMRTbell Template Prep Kit 1.0 (Pacific Biosciences) and sequenced on the PacBio RSII with DNA polymerase version P4 and sequencing chemistry version C4 for 360 minutes.