DNA methylome by RRBS of CD4+ and CD8+ T cells in Acute Coronary Syndrome patients
2 g of gDNA were used for each library preparation. DNA samples were digested by MspI restriction enzyme and purified with the GeneJet PCR Purification Kit (Thermo Fisher Scientific). All libraries were prepared by TruSeq Library Prep Kit (Illumina) and bisulfite conversion was obtained using the EZ DNA Methylation-Gold Kit (Zymo Research). DNA amplification reaction was performed using PfuTurboCxHotstart DNA Polymerase (Agilent Technologies, USA) and the amplified fragments were purified by AMPure XP Beads. Finally, they were quantified by the Agilent 4200 TapeStation (Agilent Technologies). Each DNA library was analyzed by paired-end sequencing read (2  75 cycles) on Illumina Nextseq 500. This sample was used for DMRs analysis.