Analysis  of PI3K inhibitor resistance in HNSCC
Illumina total RNA sequencing libraries run on a NovaSeq6000 with a 300 cycle run. RNA isolated from head and neck squamous cell carcinoma cell line UM-SCC-108 treated with DMSO, 1uM Pictilisib, 0.5uM Brigatinib or 1uM Pictilisib and 0.5uM Brigatinib for 24 hours was used as input. Similarly, RNA isolated from head and neck squamous cell carcinoma cell line HSC4 treated with DMSO, 2.5uM Pictilisib, 1uM Brigatinib or 2.5uM Pictilisib and 1uM Brigatinib for 24 hours was used as input.