Metabarcoding of Fungi: rapeseed soil
2-step amplicon PCR on ITS1 region with primers ITS1_f (CTTGGTCATTTAGAGGAAGTAA) and ITS2 (GCTGCGTTCTTCATCGATGC), with incorporation of barcodes on 2nd PCR, followed by purification and quantification to build equimolar pools diluted with PhiX phage mix to 12 pM