NS5BofHCV:pt1t0gt1a
Pan-gentypic amplification with new designed primer; ONEStep RT-PCR Kit (first) + FasrStart Taq DNA (second) + AMPure XP beads (purification) + High-sens dsDNA on Qubit 2.0 (first quantification) + Ion Xpress Plus Fragment Library kit (library prep) + High sens kit on Bioanalyzer 2100 (second quantification)