Illumina amplicon 16S rRNA sequencing of Cephalotes aff. wheeleri gut microbiota
DNA was extracted from whole bodies of single ants using Qiagen DNeasy Blood and Tissue kits. Samples were submitted for amplicon sequencing of the V4 hypervariable region of the 16S rRNA gene following the protocols of the Earth Microbiome Project. Library preparation and sequencing were performed at Argonne National Laboratory (Argonne, IL, USA), with paired-end 151 bp sequencing reactions on multiplexed samples on the Illumina MiSeq lanes.