Udiversus Iso-Seq libraries
Iso-Seq libraries were constructed from a total silk gland tissue RNA extraction following the Pacific Biosciences Iso-Seq protocol using the SMARTer PCR cDNA Synthesis kit. Amplification of cDNA was done with 16 cycles for 4 reactions and 18 cycles for another four reactions. Amplification products were combined and purified using normal-phase 2x AMPure XP.