RNA-Seq of exosome-derived lncRNAs: PMOP patient 1
The isolated exosome RNA was incubated with Ribo-Zero rRNA remove beads to deplete the ribosomal RNA (rRNA) and fragmented into small pieces. The fragmented RNA was ligated to 5 adapter and then, reverse transcribed with tagged random hexamer, and the cDNAs was ligated to adapter with Unique Molecular Identifiers (UMI). Then, several cycles of PCR amplification were performed to build a library.