hmC-seal of homo sapiens neuroblastoma tumor
After ligation with sequencing adapters using the KAPA Hyper Prep Kit cell free DNA was subjected to T4 b-glucosyltransferase enzymatic modification with UDP-N3-glucose, followed by subsequent chemical modification with biotin-PEG4-dibenzocyclooctyne. Biotin-labeled cfDNA fragments were then pulled down using streptavidin M270 Dynabeads, and PCR amplified to construct sequencing libraries which were purified with AMPure XP beads to construct libraries. Fifty base-pair, paired-end libraries were sequenced on an Illumina NextSeq 500 per manufacturer's instructions.