Ithomia salapia Chromium 10X sequences
The two individuals used for the genome of I. salapia were extracted following a protocol adapted from (Mayjonade et al. 2016). Samples were snap frozen alive in liquid nitrogen and conserved at -80C. DNA was extracted from the whole butterfly bodies with the exception of the head. Butterflies were ground in a frozen mortar with liquid nitrogen, 150 mg of tissue powder was mixed with 900 l of preheated buffer and 6 l of RNaseA. Tubes were incubated for 120 minutes at 50C for lysis, and then at -10C for 10 minutes, with the addition of 300 l of Potassium acetate for the precipitation. One volume of binding buffer was added with 100 l of Serapure beads solution. 3 washing cycles were used and DNA was resuspended in 100 l of EB buffer. Library construction including adaptor ligation and size selection were performed according to the manufacturers instructions. The two 10x Chromium Genome Library libraries were sequenced on one lane of the HiSeq 2500 with a 250PE-RR read metric.