single-cell ATAC-seq of Mus musculus: adult female brain cortex
Cell nuclei were isolated with ice-cold Omni-ATAC cell lysis buffer. Accessible chromatin were captured with using 25 L transposition mix (12.5 L 2 x TD buffer from Nextera kit, 10 L 1 x PBS (pH 7.4), 0.25 L 1% Digitonin, 0.25 L 10% Tween, 2 L 1.25 M META transposome), then nuclei were sorted to 96-well plates, accessible fragments were released with an ionic detergent (SDS), then amplified with META primers and barcoded. Then a whole 96-well plate was pooled and sequenced.