RNA-Seq of Larix sibirica: autumn buds
RNA was isolated from autumn buds from a reference Siberian larch tree using the Qiagen RNeasy Mini Kit (Qiagen, Hilden, Germany). The RNA-Seq library was prepared using the TruSeq RNA Sample Preparation Kit v2 (Illumina Inc., San Diego, CA). The sequencing of the obtained library was carried out on the MiSeq Illumina platform using the MiSeq Reagent Kit v2 (300-cycles) (Illumina Inc., San Diego, CA). FastQC software v. 0.11.9 was used to evaluate the quality of the sequencing data. The raw sequencing data were processed using Trimmomatic program v. 0.39 (9-bp headcrop, minimum read quality of Q=23, and minimum read length of 35 bp). SortMeRNA version 4.0.0 was used for ribosomal RNA removal. In addition, Rcorrector was used for sequencing error correction.