RNA-Seq library from shoot sample from Arabidopsis thaliana
Tissue was ground in liquid N2 and RNA extracted with Plant RNA Reagent (Life Technologies; http://www.lifetechnologies.com). Total RNA was treated with 1X RNA Secure Reagent (Ambion; http://www.lifetechnologies.com) at 65C for 10 min, treated with DNase I (Ambion; http://www.lifetechnologies.com) at 37C for 10 min, and purified with RNeasy kit (Qiagen; http://www.qiagen.com) following the manufacturer's instructions. Purity and concentration of the resulting mRNAs was determined using the Agilent Bioanalyzer 2100 (Agilent Technologies; http://www.agilent.com). Only samples with RIN > 8.0 were used. RNA-Seq library preparation and sequencing were preformed at the Oregon State University Center for Genome Research and Biocomputing (https://cgrb.oregonstate.edu). Libraries were sequenced as single-end 51bp reads on the Illumina HiSeq 2000 using a single lane.