Rumen solid of steer 251B grazing MaxQ-1; middle of the mat (replicate = 1)
DNA was extracted using mixtures phenol, chloroform, and isoamyl alcohol (25:24:1, v/v/v) and chloroform and isoamyl alcohol (24:1, v/v) method followed by isopropanol precipitation. Amplification of 16S rRNA hypervariable region 4 (V4) using 515F (Parada et al., 2016) and 806R (Apprill et al., 2015)primers. Library was prepared according to Illumina Metagenome MiSeq sequencing protocol and sequenced using Illumina MiSeq.