16S rRNA amplicon sequencing of freshwater metagenome from a dimictic lake
Water was filtered in series through an 11 m nylon and 0.22 _m PES filters to capture eukaryotic algae and bacteria (and archaea), respectively. DNA was extracted from the 0.22 _m filter using a DNeasy PowerBiofilm kit. Illumina MiSeq (2x300 bp) chemistrywas used to sequence the V4 region of the 16S rRNA gene with the primer pair 515F (5-GTGCCAGCMGCCGCGGTAA-3) and 805R (5-GACTACVSGGGTATCTAAT-3) using a dual index approach.