RNA seq of liver largemouth bass
The total RNA was extracted from livers with the Animal Total RNA Isolation Kit (Foregene, Chengdu, China) according to the manufacturers instructions. RNA was quantified and quality was assessed using the NanoDrop Spectrophotometer 2000c (NanoDrop Technologies, Wilmington, DE, USA) and 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA). The RNA of nine samples from each group were used for library construction, where optical density (OD) ratios of 260/280 and 260/230 were greater than 1.8 and the RNA integrity number (RIN) was greater than 16.