DNA-Seq of Chryseobacterium indologenes: CSF of a 3-month-old female baby hospitalized at the Neonatal Intensive Care Unit (NICU) of Hospital Geral de Palmas, Palmas, Tocantins, Brazil
Genomic DNA extraction was done from an overnight culture using the Wizard Genomic DNA Purification Kit (Promega, Madison, WI, United States), according to manufactures instructions. The concentration of the DNA extract and purity was determined by measuring absorbance at wavelengths of 260 nm and 280 nm (NanoVue Plus; GE Healthcare Life Sciences, Marlborough, MA, United States). The integrity of genomic DNA was tested by way of electrophoresis. Subsequently, the concentration of bacterial DNA was determined by the Qubit 3.0 Fluorometer equipment (Life Technologies, Carlsbad, CA, USA, and Nextera XT DNA Library Prep (Illumina, San Diego, CA, United States) was used for library preparation and genome sequencing of the isolate was done by Illumina NextSeq 550 System Sequencing (Illumina, San Diego, CA, United States) with a 2 x 75 paired-end strategy.