RadSeq of Timema douglasi: gene flow and parthenogenesis
ddRAD libraries were prepared with the enzymes EcoRI and MseI, and a 200-450bp size selection after addition of Illumina TruSeq indexes.  96 samples were pooled in each library thanks to barcoded adapters and libraries were multiplexed by pairs on the same Illumina lane using Illumina TruSeq indexes iA06 or iA12. 100bp Single-end sequencing was performed using Illumina Hiseq 2500.