DNA from shrimp larvae was extracted with the ZymoBIOMICS DNA Microprep kit (ZymoResearch, USA). The 16S rRNA gene was amplified with primers 341F/806R and the amplicons were sequenced using the Illumina NovaSeq P250 sequencing platform.
PCR were performed by specific primers connecting with barcodes. The construction of the DNA libraries was the processes of end repairing, adding A to tails, purification and etc. Libraries was performed on a paired-end Illumina platform to generate 250bp paired-end raw reads.