WGS of Listeria monocytogenes from Retail Deli Environment
gDNA was extracted from 10 mL overnight culture in BHI at 37C, 200rpm. A standard phenol-chloroform extraction method was used (28). Concentration and purity of gDNA were assessed through UV-Vis spectrophotometry on the NanoDrop 2000c (ThermoScientific, Wilmington, DE). gDNA libraries were prepared using the Nextera XT DNA Sample Preparation kit and Nextera Index kit were used for indexes labelling (Illumina, Madison, WI). The Illumina MiSeq DNA sequencing by synthesis sample preparation and workflow platforms were used to sequence sheared gDNA (conducted at the Purdue Genomics Core Facility). Paired-end, 300bp reads were performed yielding with > 3 million reads per genome.