RNA-Seq of C. gloeosporioides (Control_3)
8- and 24-h-after treatment P. patens colonies infected with C. gloeosporioides were recovered, frozen using liquid nitrogen, and pulverized. For controls, C. gloeosporioides colony grown over celophan disk on PDA medium, were recovered and treated as described before. Total RNA was extracted using the RNeasy Plant Mini Kit (Qiagen, Germany). RNA quality control, library preparation and sequencing were performed by Macrogen Inc. (Seoul, Korea). Briefly, RNA integrity number (RIN > 9) was checked using an Agilent Technologies 2100 Bioanalyzer (Agilent Technologies) and cDNA libraries were prepared for paired-end sequencing using 1 g of RNA and the TruSeq Stranded Total RNA Plant LT Sample Prep. Finally, sequencing was performed on Illumina platform (Illumina, California, USA) to generate paired-end 150 bp reads, obtaining ~50 M reads per sample with Q20 > 97.5 %.