Iso-seq of Caulerpa lentillifera:  chloroplast
Total RNA of different treatment was isolated with the RNAprep pure plant kit (Tiangen Inc, Beijing, China) and treated with RNase-free DNaseI (Tiangen Inc, Beijing, China) following the manufacturers instructions. The quality of RNA was checked on a Bioanalyzer 2100 system (Agilent, Palo Alto, CA, USA).  Then extracted RNAs from different treatments were pooled evenly, mRNA libraries were constructed with SMARTer PCR cDNA Synthesis Kit (Clontech) and sequenced on the PacBio Iso-Seq platform. Reads related to chloroplast were extract by BLAST searches.