Whole Exome Sequencing of Patient Derived Xenograft of a Pediatric Gastroenteropancreatic Neuroendocrine Tumor
DNA was extracted from tumor sample using Qiagen DNAeasy Kit (ref# 69504). An Agilent SureSelect Clinical Research Exome kit was used to to generate the genomic library. Briefly, DNA was enzymatically fragmented, tagged with adaptors, and then amplified. The The library was then hybridized with biotinylated RNA baits. The hybrids were pulled down with streptavidin-coated magnetic beads and washed. Indexes were added to enrich the library using on-bead PCR.