16s rRNA sequencing (V1 and V2 regions) of Tribolium castaneum larvae:Oral_Bttol_8
For sequencing, variable regions V1 and V2 of the 16S rRNA gene within the DNA samples were amplified using the primer pair 27F-338R in a dual-barcoding approach as per Caporaso et al. 2012.  3.5 l of cDNA was used for amplification and PCR products were verified using the electrophoresis in agarose gel. PCR products were normalized using the SequalPrep Normalization Plate Kit.