SM4_16hr
The obtained RNA was segmented by interrupting buffer and reverse transcribed with random N6 primers, and then double-stranded cDNA was synthesized to form double-stranded DNA. Connect the synthetic double strand to a connector. The ligated products were amplified by PCR with specific primers. The PCR product was thermally denatured into a single strand, and then the single strand DNA was cyclized with a segment of bridge primers to obtain a single strand cyclic DNA library.