GMNL-653 sequencing by NGS
For the L. paracasei strain GMNL-653, library preparation was conducted in accordance with the Illumina TruSeq Nano DNA Sample Preparation Kit protocol. First, 200 ng of genomic DNA was sonicated to approximately 550 bp. Fragmented DNA was processed to convert the overhangs into blunt ends, and the library size was selected using beads. After ends repair, a single A nucleotide was added to the 3 ends of the blunt fragments, and the corresponding single T nucleotide on the adapter with index was ligated to the fragments. The library size was verified through electrophoresis, and the quantification was validated with the NanoPhotometer. All validated libraries were mixed, denatured, and diluted to the appropriate concentration for pair-end 250-cycle sequencing on MiSeq.