RNASeq from head of Ithomia salapia
Tissue samples were homogenised in 600 l of RLT buffer with TissueLyser (Qiagen, Hilden, Germany). Total RNA was then extracted according to the manufacturers protocol (RNeasy Mini kit, Qiagen, Hilden, Germany) and eluted in 30 l of RNase-free water. To avoid genomic contamination, RNase-free DNase treatment (Qiagen, Hilden, Germany) was performed during RNA extraction. The quality of the isolated RNA was checked on 0.8% agarose gel for the presence of 28S and 18S bands. The quality and quantity of RNA was further analyzed using Qubit 2.0 fluorometer (Invitrogen, Carlsbad, CA, USA) and RNA integrity was confirmed using an Agilent Bioanalyser 2100 (Agilent Technologies, CA, USA). Libraries were sequenced with Illumina HiSeq 2500 platform.