Single cell RNA profile of term umbilical cord blood mononuclear cells
Frozen UCBMC (n=2/group) were thawed and enriched for live cells using SYTOX Blue (1:1000, ThermoFisher) on the BD FACS Aria Fusion into RPMI (supplemented with 30% FBS). Sorted cells were counted in triplicates and resuspended in PBS with 0.04% BSA in a final concentration of 1200 cells/uL. Cells were immediately loaded in the 10X Genomics Chromium Controller with a loading target of 17,600 cells. Libraries were generated using the V3 chemistry per manufacturers instructions (10X Genomics, Pleasanton CA). Libraries were sequenced on Illumina NovaSeq with a sequencing target of 50,000 reads per cell.