16S sequencing of Paneth cell-specific IL-22Ra1 knockout mice.
Terminal ileum luminal contents DNA from littermate cohoused Il22Ra1fl/fl;Defa6-cre+ (n=4) mice and cre- (n=4) mice (6 weeks age) were isolated using QiAMP stool DNA extraction kit. Microbial community analysis utilized PCR amplification of the V4 region of 16S rRNA followed by sequencing on an Illumina MiSeq was performed and analyzed by MR DNA (pay for service).  KO mice designated as Defa.creplus and WT as Defa.creminus