16S rRNA gene V3-V4 hypervariable regions: fishery pond sediments
Total genomic DNA was isolated from sediment samples using the ZymoBIOMICS  DNA Miniprep Kit (Zymo Research, USA) according to the manufacturer's recommendations. The concentration of extracted DNA was evaluated using a biophotometer (Eppendorf, Germany). The DNA samples were sent to Novogene Bioinformatics Technology Co., Ltd. (Beijing, China) to amplify V3-V4 16S rRNA regions and sequence on Illumina paired-end platform to generate 250 base pairs (bp) length paired-end raw reads. Note: the uploaded sequences are with adapter and barcode sequences removed.