T883_N, NrasG12D
Total bone marrrow RNAs were extracted using Qiagen RNeasy Mini kit plus on-column DNase digestion per the manufacturers instructions. The concentration of RNA was determined by Invitrogen Qubit Fluorometer and the integrity of RNA passed the examination by Agilent 2100 Bioanalyzer. 2.5 g of total RNA were processed to construct cDNA libraries using TruSeq RNA Sample Preparation v2 Kit (Illumina, RS-121-2001) following the manufacturers protocol. The concentration and integrity of the cDNA libraries were assessed with Invitrogen Qubit Fluorometer and Agilent 2100 Bioanalyzer, respectively. The next generation deep sequencing was performed on a HiSeq 2000 sequencer (Illumina, SY-401-1001).