Whole genome of BR19001YR
Purified genomic DNA was randomly sheared to yield DNA fragments of average 350 bp in size using a Covaris S2 Ultrasonicator. Library preparation was performed following the Illumina TruSeq DNA PCR-free preparation Kit. Adaptor enrichments was performed using PCR according to the manufacturers instructions. The final library size and quality were evaluated electrophoretically with an Agilent High Sensitivity DNA Kit. The 150bp paired-end reads were sequenced on Illumina NovaSeq 6000 platform. Further image analysis and base calling were performed with RTA3 (Real Time Analysis) and bcl2fastq v2.20.0.422.