complete chloroplast genome of Platycodon grandiflorus from Shanxi
Total genomic DNA was randomly fragmented by the Covaris M220 ultrasonic breaker for library construction (Qiu et al. 2020). The constructed library was paired-end sequenced using Illumina NovaSeq platform (Qiu et al. 2020). Raw reads were trimed using Trimmomatic V.0.35 (Xu et al. 2021). The optimized sequence was assembled by NOVOPlasty (https://github.com/ndierckx/ NOVOPlasty) (Yaradua et al. 2019). The gaps of the assembly results were filled by GapCloser v1.12 software. The chloroplast genome of P. grandiflorus was annoted using the Dual Organellar GenoMe Annotator (DOGMA) software (https://dogma.ccbb. utexas.edu/) (Zhang et al. 2019). The annotations were manually reviewed and edited by comparison with Codonopsis tsinlingensis (GenBank accession MN122102).