Microbiome analysis of uninfested rice tissue
Region V3-V4 was PCR amplified from genomic DNA of gall midge infested (GT) and uninfested (GC) rice tissues, gel purified and library generated using Nextera XT DNA Library Prep Kit and Nextera XT index kit. The library was cleaned by magnetic beads and quantified and analysed by Bioanalyser (Agilent) and sequenced using Illumina MiSeq sequencing platform.