study of 16R rDNA gene of Irritable bowel syndrome patients
V4 region of 16S rRNA gene was targeted for bacterial species, using universal primers: 515f (5-GTGCCAGCMGCCGCGGTAA-3) and 806r (5-GGACTACHVGGGTWTCTAAT-3) (Gohl, Vangay et al. 2016). Illumina MiSeq plant form using 2300-bp paired end protocol, was followed for sequencing