YSCM1
The isolated genomic DNAs, together with 0.5% unmethylated lambda DNA (Thermo Scientific), were subjected to bisulfite conversion using an EZ DNA Methylation-Direct Kit (Zymo Research, D5020) according to the manufacturers protocol (Invitrogen). Amplified libraries were purified using 0.8 Agencourt Ampure XP beads twice, according to the manufacturers guidelines, and were assessed on the Agilent Bioanalyzer 2100 platform, and the KAPA Library Quantification Kit for Illumina (KAPA Biosystems). Sequencing was performed on Illumina HiSeq 2500 machines for 150 bp paired-end sequencing.