18S rRNA amplicon sequencing of freshwater metagenome from a dimictic lake
Water was filtered in series through an 11 m nylon and 0.22 _m PES filters to capture eukaryotic algae and bacteria (and archaea), respectively. DNA was extracted from the 0.22 _m filter using a DNeasy PowerBiofilm kit. 18S rRNA sequencing for eukaryotic algae was performed on an Illumina MiSeq using 2x250 bp chemistry. The V9 region 18S rRNA was amplified using primers 1391F (5-GTACACACCGCCCGTC-3) and EukBr (5-TGATCCTTCTGCAGGTTCACCTAC-3).