RNA-seq of Paroster macrosturtensis
A single specimen was preserved in liquid nitrogen and total RNA was extracted from the entire animal with an AllPrep DNA/RNA Micro kit (Qiagen) according to the standard protocol for tissue extraction. Library preparation was performed using a Nugen Universal Plus mRNA-Seq Library Preparation Kit (Tecan, Mnnedorf Switzerland), following manufacturers instructions. All the barcoded cDNA samples were pooled and 100 bp paired-end sequencing was outsourced to the Australian Genome Research Facility (AGRF) on an Illumina NovaSeq 6000 S4 lane.