HaloPlex Sequencing of human UPN 303210 tumor MDS: Sample UPN-303210_MDS_haloplex
The HaloplexHS kit (Agilent Technologies) was used for error-corrected sequencing. To create libraries for error-corrected sequencing, 500 ng of DNA was digested with a custom set of restriction enzymes before hybridization to probes against the target regions, plus 10 base pair degenerate oligonucleotides [unique molecular indexes (UMIs)] and sample specific indexes. Following magnetic bead enrichment, libraries were amplified with PCR using primers with tailed Illumina sequencing motifs to generate libraries