Industrial Dahi Metagenome DNA brand
Adapter-ligated DNA was purified using HiPrep clean up beads. Resultant fragments were amplified for 8 cycles of PCR using Illumina-compatible primers provided in the NEXTflex Rapid DNA sequencing Bundle. Final PCR product was purified with HiPrep cleanup beads followed by library quality control. Sequencing library was quantified by Qubit fluorometer and the fragment size distribution was analyzed on Agilent 2200 TapeStation