RNA-Seq of mus musculus: female liver
Hepatospheres were formed by culturing purified mouse hepatocytes, isolated by 2 months old female C57Bl6 mice, at a density of 5x105 cells/ml in 60 l hanging drops in Williams E medium with supplements. Hepatospheres were cultured for 24h in the presence of 5ng/ml recombinant mouse TGF1. A pull of 8 hepatospheres was used for each library preparation.