Genome sequencing of Pleurotus ostreatus collected at the Massane Forest
The genomic DNA is randomly fragmented to a size of 350bp by shearing, then DNA fragments were end polished, A-tailed, and ligated with the NEBNext adapter for Illumina sequencing, and further PCR enriched by P5 and indexed P7 oligos. The PCR products were purified (AMPure XP system) and resulted libraries were analyzed for size distribution by Agilent 2100 Bioanalyzer and quantified using real-time PCR. Paired-end sequencing was performed using Illumina (NovaSeq 6000) at a coverage of 20X and a read length of 150bp for each sample.