Deep targeted DNA sequencing for the identification of minimal residual disease.: Sample 05055_81_cfDNA_Y1
cfDNA was extracted from fresh or archival plasma of healthy donors or cancer patients. gDNA was extracted from buffy coats or cell lines, sheared, and quantified. DNA libraries were constructed from 2-20 ng DNA inputs by using the Kapa Hyper Prep kit (Kapa, kit #KK8504) with custom dual index duplex UMI adapters (IDT).