16S rRNA amplicon sequencing of freshwater metagenome from a dimictic lake
Between 75-100 ml of water filtered onto 0.22 _m filters. DNA was extracted with DNEasy Powersoil DNA Isolation kit. DNA was quantified with a Nanodrop. Illumina MiSeq (2x300 bp) chemistrywas used to sequence the V4 region of the 16S rRNA gene with the primer pair 515F (5-GTGCCAGCMGCCGCGGTAA-3) and 805R (5-GACTACVSGGGTATCTAAT-3) using a dual index approach.