RNA-Seq of human: adult unspecified bone_marrow tumor: Sample 184874_scRNA
Cells were processed using the 10x Genomics Chromium Controller and the Chromium Single Cell Library 10x Genomics Single Cell RNA-sequencing Gel Bead Kit following the standard manufacturer‚ÄövÑv¥s protocols (https://tinyurl.com/y96l7lns). Gel beads were prepared according to standard manufacturer‚ÄövÑv¥s protocols. Oil partitions of single-cell‚ÄövÑv¢+‚ÄövÑv¢oligo coated gel beads (GEMs) were captured and reverse transcription was performed, resulting in cDNA tagged with a cell barcode and unique molecular index (UMI). Next, GEMs were broken and cDNA was amplified and quantified using an Agilent Bioanalyzer High Sensitivity chip (Agilent Technologies). To prepare the final libraries, amplified cDNA was enzymatically fragmented, end-repaired, and polyA tagged. Fragments were then size selected using SPRIselect magnetic beads (Beckman Coulter). Next, Illumina sequencing adapters were ligated to the size-selected fragments and cleaned up using SPRIselect magnetic beads (Beckman Coulter). Finally, sample indices were selected and amplified, followed by a double sided size selection using SPRIselect magnetic beads (Beckman Coulter). Final library quality was assessed using an Agilent Bioanalyzer High Sensitivity chip.