PacBio long-read seq of EWSR1 and EWS-FLI1 from siFLI1 TC-32 cells
Total RNA was extracted from 3 biological replicates using the Maxwell 16 LEV simplyRNA Cells kit (Promega) and pooled to form one single sample. 1ug of RNA was provided to the National Cancer Institute Center for Cancer Research Sequencing Facility for library prep and long-read sequencing. Protocols can be found here: https://ostr.ccr.cancer.gov/resources/sequencing-facility/