18S Amplicon sequence of Plastic bacterial  community collected from Bolinao on Mar 9, 2019
Environmental water samples were collected alongside in situ experiment (approx. 1m depth) and pre-filtered using 120um sieve. DNA was isolated from the biofilm from the PE plastics using PowersSoil DNA Isolation Kit. The V4 region of the 18S rRNA gene was amplified using the forward primer E572F and reverse primer 1009R. Sequencing was carried out using Illumina MiSeq  at the Integrated Microbiome Resource, Dalhousie University, Canada.