16S rRNA gene-seq of intestinal microbiome:  bioreactor bovine rumen model
Frozen bioreactor samples were processed for bacterial DNA isolation using QIAamp Stool Mini Kit (Qiagen) according to the manufacturers instructions, and bacterial DNA samples were submitted to Starseq (Mainz, Germany) for bacterial 16S DNA V3-V4 amplicon sequencing using the Illumina MiSeq PE300 platform. Following primers were used for library preparation: forward primer: 341F:ACTCCTACGGGAGGCAGCAG; reverse primer: 806R:GGACTACHVGGGTWTCTAAT.