RNA-seq of homo sapiens: kidney cells
RNASeq libraries were generated using Ribo-Zero Plus rRNA Depletion Kit (Illumina) kits per manufacturers instructions. Individual libraries were assessed for quality using the Agilent 2100 Bioanalyzer and quantified with a Qubit Fluorometer. Paired-end sequencing of multiplexed libraries was performed on the Hiseq 4000 platform (Illumina) using PE75 read lengths by the Vanderbilt Technologies for Advanced Genomics (VANTAGE) core (Vanderbilt University, Nashville, TN).