Genome sequence of H. exemplaris, pooled adult females
DNA was isolated using DNA purification kits from Qiagen (Qiagen Inc., Germantown, MD, USA). Library synthesis was done using the automated Apollo 324 NGS Library Prep system with the PrepX DNA-Seq kit (Wafergen, Fremont, CA, USA). Final product of each library was subjected to <12 cycles of PCR enrichment in reactions containing one barcode for each sample. Final libraries were purified with magnetic beads to an average size of 500 bp. The multiplexed mix was submitted to 150 cycles of paired-end sequencing (2x250bp) using HiSeq 2500 (Illumina, San Diego, CA, USA).