ITS2 & 28S D2 amplicon-seq Vredepeel soil
ITS2 and 28S D2 region PCR, amplicon cleanup, random FA fragment size check, ITS2 and 28S amplicons pooled per sample, Illumina Nextera barcoded 2nd PCR, cleanup, QC, quantification, equimolar pooling for MiSeq V3 2x300 nt PE, demultiplexed read data, ITS2 and 28S D2 reads were split with use of primer sequences