16s rRNA V3-V4 Metagenome of VCO Fermentation: Layer 2
The 16S rRNA variable regions V3 and V4 were amplified using the Herculase II fusion DNA polymerase Nextera XT Index Kit (Illumina). The PCR products were pooled together in equimolar ratios with a final concentration of 20 pmol/L each. These pooled samples were sequenced using the Illumina-MiSeq  platform.