RNA-Seq of MIN6 cells treated with cyto mix
Total RNA was extracted by trizol. Oligo(dT)-attached magnetic beads were used to enrich mRNA, which subsequently fragmented into small pieces. The First-strand cDNA was generated using random hexamer-primed reverse transcription, followed by a second-strand cDNA synthesis and addition of A-Tailing Mix and RNA Index Adapters. The cDNA fragments were then denatured and circularized into single strand circle DNA (ssCir DNA). The final library was amplified with phi29 to make DNA nanoball (DNB) .