GSM5009186: Adipose derived cells Day 10; Mus musculus; RNA-Seq
For the generation of single-cell gel beads in emulsion, cells were loaded on a Chromium single cell instrument (10x Genomics) with an estimated targeted cell recovery of ~5,000 cells as per manufacturer's protocol. Cells were partitioned with Gel Beads into emulsion in the Chromium instrument where cell lysis and barcoded reverse transcription of RNA occurred following amplification. Single cell RNA-Seq libraries were prepared by using the Chromium single cell 3′ library and gel bead kit v3 (10x Genomics).