RNAseq of Prunus persica: mature fruit
Total RNA extraction was carried out using the Agilent Total RNA Isolation Mini Kit. Cytoplasmatic rRNA removal was performed using the Ribo-Zero rRNA Removal Kit (Epicentre, Madison, WI, USA). rRNA-depleted RNA was used to prepare strand oriented libraries using the TruSeq Stranded Total RNA Sample Prep Kit (Illumina, San Diego, CA, USA). Sequencing was performed on an HiSeq2500 sequencing platform (Illumina, San Diego, CA).