GSM6284673: Epidermis_scRNAseq_1; Homo sapiens; RNA-Seq
A healthy whole skin biopsy was obtained from a 30 y/o male donor of fair-skin type and preserved in MACS Tissue Storage Solution (Miltenyi Biotec). The skin sample was subsequently cut into small pieces that were further processed using the human Epidermis Dissociation Kit (Miltenyi Biotec) and the Gentle MACS Dissociator (Miltenyi Biotec), following the manufacturer's instructions. The resulting cell suspension was then filtered through a 70-µm cell strainer (Falcon) and depleted of apoptotic and dead cells with the Dead Cell Removal Kit (Miltenyi Biotec). We used the 10X Genomics platform to generate a sequencing library with the Chromium Single Cell 3' Reagent Kit, v2 chemistry (10X Genomics), as described by the manufacturer. Approximately 20,000 cells were loaded into a Chromium Single Cell Controller (10X Genomics) as initial input.