Amplicon sequencing of diatom assemblages: seawater biomass filters
Seawater plankton community biomass filters extracted for DNA and amplified for diatom assemblages using the V4 region of the 18S rDNA with Illumina MiSeq adapters attached to primers. Samples were multiplexed using two unique sets of Illumina indeces on the same 2x250 bp Illumina MiSeq run at the Rhode Island Genomics & Sequencing Center. These reads have been demultiplexed, the Illumina MiSeq Nextera adapter sequences removed, and reads were separated by primer sequence and the the primer sequence was removed. The forward primer used was D512F: ATTCCAGCTCCAATAGCG and the reverse primer was D978R: GACTACGATGGTATCTAATC (Zimmermann et al 2011).Primers are reported from 5' to 3'.