GSM6171565: OctA-2_H3K27ac; Homo sapiens; ChIP-Seq
Cells were fixed with 1% paraformaldehyde for 10-mins and quenched by glycine for 2-mins. After cell lysis, genomic DNA was sonicated by Branson digital 450 cell sonifier. The DNA library was prepared using NEBNext Utra II DNA Library Prep with Sample Purification Beads (NEB #E7103S) according to the manufacture's instruction.