GSM5114383: Spleen_Il1b_GAS_1; Mus musculus; RNA-Seq
Total RNA was isolated using 1 ml TRIzol reagent (Life Technologies) according to the manufacturer's protocol. Contaminating DNA was removed by digestion with 20 U recombinant DNase I (Roche). RNA-Seq library was prepared using Automated library preparation and multiplexing with the QuantSeq 3' mRNA-Seq Library Prep Kit (Lexogen).