16S rRNA sequencing of mice gut microbiome
Total RNA was extracted from the tissue using TRIzol Reagent according the manufacturers instructions (Invitrogen) and genomic DNA was removed using DNase I (TaKara). RNA-seq transcriptome library was prepared following TruSeq RNA sample prep Kit (Illumina, San Diego, CA, USA), and was subsequently sequenced with the Illumina HiSeq xten.