GSM5233642: Input DNA; Homo sapiens; ChIP-Seq
Approximately 5 x 10^7 cells were used for each ChIP-Seq assay. The chromatin DNA precipitated by polyclonal antibody against CARM1. The DNA was purified with a Qiagen PCR purification kit. A Vazyme TruePrep DNA Library Prep Kit V2 for Illumina ® (Vazyme Biotech) was used for DNA library construction. Briefly,the kit uses a new-type transposasea for DNA fragmentation and ligation  of Illumina's adapters. After adapter ligation DNA was PCR amplified with Illumina primers for 15 cycles and library fragments of ~250 bp (insert plus adaptor and PCR primer sequences) were band isolated from an agarose gel. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on an Illumina sequencer following the manufacturer's protocols.