PacBio Sequel SMRT WGS of Sandy the Desert Dingo: skin biopsy
Genomic DNA was prepared from skin biopsy with supplemental RNase (Astral Scientific, Taren Point, Australia) and proteinase K (NEB, Ipswich, MA, USA) treatment, as per the manufacturer's instructions. Isolated gDNA was further purified using AMPure XP beads (Beckman Coulter, Brea, CA, USA) to eliminate sequencing inhibitors. 150ng of DNA was size selected by the Sage Science Pippin Pulse (0.75% KBB gel; 9hr 10-48kb (80 V) program; Invitrogen 1kb Extension DNA ladder). Two libraries were prepared and sequenced on Sequel machines with 2.0 chemistry recording 10 h movies at Arizona Genomic Institute, University of Arizona.