RNA-Seq ovary
The RNA-Seq paired end sequencing libraries were prepared from the QC passed RNA samples using illumina TruSeq Stranded mRNA sample Prep kit. Briefly, mRNA was enriched from the total RNA using poly-T attached magnetic beads, followed by enzymatic fragmentation, 1st strand cDNA conversion using SuperScript II and Act-D mix to facilitate RNA dependent synthesis. The 1st strand cDNA was then synthesized to second strand using second strand mix. The dscDNA was then purified using AMPure XP beads followed by A-tailing, adapter ligation and then enriched by limited no of PCR cycles.