GSM5657420: ChIPseq INPUT D56A Rep B; Cereibacter sphaeroides 2.4.1; ChIP-Seq
Cells lysed and DNA sheared with sonication, DNA-protein fragments trimmed with micrococal nuclease and Rnase treated, no immunoprecipition was performed on Input, crosslinking reversed with heating, and DNA purified with QIAquick PCR purification kit ChIP-seq library preparation and sequencing was performed at GeneWiz. Libraries for sequencing were created using the Illumina TruSeq ChIP library preparation kit (Illumina) following the standard protocol. ChIP-seq libraries were sequenced on an Illumina HiSeq 3000 2x150bp reads using the standard protocol.