GSM5825864: mESC_ChIP-seq_ATRX_scramble_KD_rep1; Mus musculus; ChIP-Seq
For ATRX and H3K9me3 ChIP-seq experiments, TERRA KD or scramble KD cells were fixed with 1% formaldehyde for 5 min at RT. Chromatin were sonicated to 200-300 bp by Covaris S220. Immunoprecipitations were performed overnight at 4℃. Sample without antibody capture was used as input control. After incubation overnight, immunocomplexes were incubated with Dynabeads Protein G (Thermo Fisher Scientific, 10004D) for 2 hours at 4℃. Immunocomplexes were washed by RIPA buffer containing 500 mM of NaCl. Immunoprecipitated DNA were purified by using PCR DNA isolation system (Viogene, GP1001) following manufacturer' s protocol. For ChIP-seq, DNA library were constructed using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645S) according to manufacturer' s protocol.