GSM5588886: LuCaP81 castrated Testosterone; Homo sapiens; RNA-Seq
100mg of tissue was homogenized in TRIzol Reagent (Invitrogen) using a Polytron PT1300D homogenizer at speed 15. RNA extraction was performed using MagMAX™-96 for Microarrays Total RNA Isolation Kit (Invitrogen). Libraries were prepared by the Columbia Genome Center. Poly-A pull-down was used to enrich mRNAs from total RNA samples (200ng-1ug per sample, RIN>8 required). Libraries were prepared using the Illumina TruSeq RNA prep kit.