Pacbio reads of Enterobacter cloacae CMCC(B)45301 genome
The library was constructed using the SMRT bell TM Template kit v1.0. The extracted DNA was fragmented to a size of ~10 kb using Covaris g-TUBE and the damages and ends of the fragments were subsequently repaired. The hairpin adapters were then ligated to the DNA ends, followed by purification of the fragments using AMPure PB beads and size-selection using the BluePippin system. The selected SMRT bell templates were then subjected to damage repairment, quality determination by Qubit 2.0, and size detection by Agilent 2100. Finally, the SMRT bell templates were sequenced using the Pacbio Sequel platform.