GSM5655446: H3K14ac_ChIPseq 2; Homo sapiens; ChIP-Seq
Total RNA was isolated using a RNeasy Mini kit and treated with DNase (Qiagen). Chromatin was isolated using a SimpleChIP Enzymatic Chromatin IP kit (Cell Signaling Technology), sonicated to 200-300 bp in length, and subjected to immunoprecipitation overnight with respective antibodies. The ChIP DNA was eluted, decrosslinked, and purified. mRNA-seq libraries were prepared with a KAPA mRNA HyperPrep kit (Roche). ChIP-seq libraries were prepared with a KAPA Hyper Library Preparation kit.