GSM5589471: ChIPseq_neurons_WT_pol2_input_1; Mus musculus; ChIP-Seq
ChIP was carried out as previously described (Barisic et al., 2019) with the following modifications: (1) Tow 15 cm plates of embryonic stem cell derived Ngn2 neurons were harvested eight days after doxycycline induction (2) chromatin was sonicated for 20 cycles of 30 s using a Diagenode Bioruptor Pico, with 30 s breaks in between cycles; (3) protein A magnetic Dynabeads Magnetic beads (Invitrogen, catalog number 10008D) were used; and (4) DNA was purified using AMPure XP beads. Immunoprecipitated DNA and input DNA were submitted to library preparation (NEB Next Ultra DNA Library Prep Kit, Illumina). In the library preparation protocol, input samples (200 ng) were amplified using 6 PCR cycles and immunoprecipitation samples using 12 cycles.