Iso-Seq of Human Primary Mediastinal Large B-Cell Lymphoma : Sample IGMCH0300_S-2021-008141_PacBio_Iso-Seq
Reaction followed NEBNext Single Cell/Low Input protocol outlined within Iso-Seq Express Template Procedure & Checklist (PN 101-763-800 Version 02 (October2019)). SMRTbell library preparation used PacBio SMRTbell Express Template Prep Kit 2.0 with subsequent complexing using Sequel II Binding Kit 2.0. Loaded at 50pM on plate with 2 hr pre-extension and 24 hr movie.