GSM6123697: Minus1-to-1 randomization AQ-seq with let-7a-1 input; Homo sapiens; miRNA-Seq
Gel purification The input and uncleaved pre-miRNAs were subjected to AQ-seq as previously described (Kim et al., 2019), with some modifications as follows. After 3′ adapter ligation, the ligated RNAs were purified on a 10% urea-polyacrylamide gel along with Century-Plus RNA Markers (Thermo Fisher) as a size marker. After reverse transcription, cDNAs were amplified with NEBNext Ultra II Q5 Master Mix (NEB). The libraries were sequenced on the NovaSeq 6000 platform.