Illumina HiSeq 4000 paired end sequencing; HNF4A is required to specify glucocorticoid action in the liver - ATACseq
HNF4A is required to specify glucocorticoid action in the liver - ATACseq
Mice were treated acutely with an intraperitoneal injection of dexamethasone or vehicle, and liver tissue harvested one hour later. For wild type samples ATAC-seq was performed on flash frozen liver tissue employing Active Motif\u2019s end-to-end ATAC-seq service (https://www.activemotif.com/catalog/1233/atac-seq-service). Otherwise, ATAC-seq on flash-frozen liver tissue was performed using the Active Motif ATAC-seq kit, first isolating nuclei using the OMNI-ATAC protocol (Corces et al., 2017; https://doi.org/10.1038/nmeth.4396), subsequently following manufacturer\u2019s instructions for the kit. For wild type samples ATAC-seq was performed on flash frozen liver tissue employing Active Motif\u2019s end-to-end ATAC-seq service (https://www.activemotif.com/catalog/1233/atac-seq-service). Otherwise, ATAC-seq on flash-frozen liver tissue was performed using the Active Motif ATAC-seq kit, first isolating nuclei using the OMNI-ATAC protocol (Corces et al., 2017; https://doi.org/10.1038/nmeth.4396), subsequently following manufacturer\u2019s instructions for the kit.