GSM6171430: Rag2NE1KO1; Mus musculus; RNA-Seq
Total RNA was prepared from Rag2-/- and Rag2-/-NE1-/- pro-B cells. RNA was extracted using TRIzol (Life Technologies) and genomic DNA (gDNA) was eliminated using the genomic DNA wipeout buffer in the QuantiTect Reverse Transcription Kit (Qiagen). RNA was purified using with the RNeasy Kit (Qiagen), ribosomal RNA was eliminated using the Ribo-Zero Magnetic Gold Kit (Illumina), processed with the NEBNext Ultra Directional RNA Library Prep Kit (Illumina) and sequenced on the Illumina HiSeq system.