lncRNA-Seq of Mus Musculus: P1 Retina
Briefly, rRNAs were removed from Total RNA using EpicentreRibo-Zero rRNA Removal Kit (illumina, USA) and fragmented to approximately 200bp. Subsequently, the purified RNAs were subjected to first strand and second strand cDNA synthesis following by adaptor ligation and enrichment with a low-cycle according to instructions of NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA). The purified library products were evaluated and then diluted to 10 pM for cluster generation in situ on the pair-end flow cell followed by sequencing (2150 bp) HiSeq3000.