GSM4783808: C1; Mus musculus; RNA-Seq
CD45+ leukocytes from dissociated MC38 tumors were isolated by positive MACS selection (Miltenyi) 12 days post-implantation from female C57BL6/J mice fed control or high-fat diet for 8-10 weeks. Cells were immediately loaded on the 10X Chromium controller for single cell encapsulation. Library construction was performed as per standard 10x protocol for single-cell 3' RNA-seq v2 (Manual CG00052 Rev B)