RNA-seq of Arabidopsis:root
The  quality and quantity of the total RNA were assessed using the 2100 Bioanalyzer instrument (Agilent Technologies, Santa Clara, CA, USA). First-strand cDNA was synthesised using the First-Strand cDNA Synthesis Kit (Roche) according to the manufacturers instructions. Sequencing was performed using the BGISEQ-500 sequencer (BGI, Shenzhen, China).