RNA-seq of normal schwann cells versus vestibular schwannomas in human patients
First, a Fragment Analyzer (Advanced Analytical Technologies Inc.) confirmed the quality of RNA samples. Illumina libraries were then prepared and indexed from ~250ng of total RNA using the Kapa Hyperprep kit following manufacturers recommendations (Roche). The libraries were confirmed using the Fragment Analyzer, quantified by qPCR, then pooled and sequenced on an Illumina NextSeq500 using 40+40 paired end reads. The samples were demultiplexed by custom scripts which allowed single mismatch to the index sequencing.