CRISPRi guide sequencing in SNU16-dCas9-KRAB cells
Genomic DNA was extracted using Zymo DNA miniprep kit. Libraries were prepared using 3 rounds of PCR. Amplified products were purified using SPRIselect reagent kit (Beckman Coulter, Cat# B23318) at 1.2x sample volumes following the manufacturers protocol. Libraries were sequenced on an Illumina Miseq with paired-end 75 bp read lengths. Read 1 was used for downstream analysis.