GSM6123568: WT_Bm_4w,scRNAseq; Mus musculus; RNA-Seq
Bone marrow cells from WT and DTAhet mice were isolated by flushing the bone marrow cavities of mice femurs and tibias wit PBS. Single-cell RNA sequencing (scRNA-seq) was performed using 10X Genomics Chromium platform. Related operations including Generation of gel beads in emulsion (GEMs), barcoding, GEM-RT cleanup, complementary DNA amplification and library construction were all carried out following the manufacturer's protocol. Clustering analysis, proportion analysis, RNA velocity, myeloid trajectory analysis lymphoid trajectory analysis was performed.150-base-pair paired-end reads was applied.