GSM5655534: Tumor_PBS; Mus musculus; RNA-Seq
Cells were prepared according to 10x Genomics® Single Cell Protocols Cell Preparation Guide, followed by MACS Dead Cell Removal Kit 130-090-101 and RBC removal Single-cell transcritpome caputred by 10X 3' Reagent Kiits (v3) After running on 10X Chromium Single Cell Controller, gel beads-in-emulsion (GEMs) were generated. The barcoded and full-length cDNAs were produced after incubation of the GEMs and amplified via PCR. Library was qualified by Agilent Bioanalyzer 2100 and quantified by real-time PCR on QuantStudio3. Sequenced was done with Illumina NextSeq 550 System using High Output Kit v2.5 (50,000 reads per cell).