GSM5009209: 3_HG23_2_D6_NegC; Homo sapiens; RNA-Seq
Total RNA from hASC was extracted with Nucleospin RNA mini kit from Macherey Nagel (Macherey Nagel, Dueren, Germany) Illumina TruSeq stranded mRNA sample The yield and quality of the amplified libraries were analyzed using Qubit (Thermo Fisher) and the Agilent Tapestation (Agilent). The indexed cDNA libraries were normalized, combined and the pools were sequenced on the Illumina Nextseq 550 (Illumina) for a 75-cycle v2 sequencing run generating 75bp single-end reads