GSM5655570: Liver parenchymal cells-control; Mus musculus; RNA-Seq
Briefly, after anesthetization, mice were perfused through the portal vein with buffer solution lacking Ca2+ and Mg2+ and then perfused with 0.05% type IV collagenase at 37℃. The liver was then excised, minced and filtered through a 70 µm filter. Parenchymal cells were stored temporarily in Dulbecco's Modified Eagle Medium with 10% fetal bovine serum (FBS), 15 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), 100 U/ml penicillin, and streptomycin after digestion. Parenchymal cells were separated by centrifugation at 50 × g for 2 min twice. Cell viability was typical > 90% by trypan blue exclusion. Libraries generated using the 10× Genomics Chromium system require a conversion step using the MGIEasy Universal Library Conversion kit (App-A) (Part Number: 1000004155) before sequencing can be performed on the MGISEQ-2000 instrument.