GSM5589508: RNAseq_ESC_qko_mbd_cl2_3; Mus musculus; RNA-Seq
RNA for RNA-seq was isolated for ES cells with the RNeasy mini kit (Qiagen) using on- column DNA digestion or of neurons with Direct-zol RNA Microprep (Zymo research) with on-column DNA digestion. Sequencing libraries were prepared from purified RNA for a minimum of two biological replicates per condition using TruSeq stranded total RNA Library Prep (Illumina)