RNA-Seq of Grapholitha molesta:whole body
The first cDNA strand was synthesized in the M-Mulv reverse transcriptase system with random oligonucleotides as primers, and then the RNA strand was degraded by RNaSeh. The second cDNA strand was synthesized in the DNA polymerase I system with DNTPs as raw materials.After purification, the double-stranded cDNA was repaired at the end, A tail was added and sequencers were connected. AMPure XP beads were used to screen cDNA of about 250-300bp. PCR amplification was carried out and AMPure XP beads were used to purify the PCR products, and finally library was obtained