GSM5589780: CRC-404 PDOX, rep3; Homo sapiens; ATAC-seq
The nuclei were extracted from samples (PT, PDO, PDX and PDOX). For each sample type in one PT-PDO-PDX/PDOX set, three replciates were included. Intact nuclei (5x10^4) were used for each replicate. Transposase, digitonin, and Tween-20 were added, and the mixture was incubated at 37C for 30 minutes. The tranposed fragments were amplified. The Omni-ATAC protocol was utilized for ATAC library preparations.