GSM5825789: WT-2; Mus musculus; RNA-Seq
Total RNA extraction was performed using TRI reagent (Molecular Research Center, Inc. OH) according to the manufacturer's protocol. The RNA concentration and purity were measured by Nanodrop and Qubit (Thermo Scientific, USA). Directional RNA-seq libraries were prepared from 1 ug total RNA using the NEBNext Directional Ultra II RNA Library Prep Kit for Illumina (New England Biolabs), with initial polyA+ isolation.