Input ChIP-Seq of HMEC-hTert breast epithelial  cells treated with Bex-Carv
Cells were cross-linked in 1% methanol-free ultrapure formaldehyde for 10 min at RT. Formaldehyde was quenched by glycine to 0.125M final concentration. The fixed cells were lysed and cell nuclei pellet was collected. Chromatin was sheared by Bioruptor Plus, diagenode sonicator. Input samples were set aside as 10% of the sheared chromatin used for each IP. The protein-DNA complexes were de-crosslinked. DNA samples were purified by the addition of RNase and proteinase K. The last purification step was performed using Qiagen MinElute PCR purification kit (REF 28004).  DNA concentration was measured by the Qubit dsDNA HS assay. ChIP-seq libraries were prepared using 10 ng of DNA and Illuminas TruSeq ChIP Sample Preparation. Sequencing was performed using the Illumina's NextSeq500 instrumental mode.