ESI09
Total RNA was isolated using Trizol (Invitrogen) and the RNeasy mini kit with on-column DNase digestion (Qiagen). Sequencing libraries were prepared from 100500ng total RNA using the TruSeq Stranded RNA Sample Preparation Kit v2 (Illumina) according to the manufacturers protocol. RNA quality was confirmed using the 2100 BioAnalyzer (Agilent) and samples were normalized and pooled for sequencing on an Illumina HiSeq4000 using barcoded multiplexing and a single-end 90 bp length.