GSM5008972: Immunocastrated pigs (pooled RNA)_adipose 2; Sus scrofa; RNA-Seq
The inner layer of subcutaneous adipose tissue at withers (i.e., between first thoracal and last cervical vertebra) was sampled, immediately frozen in liquid nitrogen and stored at -80 °C. Total RNA was extracted using RNeasy plus Universal Mini Extraction Kit. Extracted RNA samples were pooled in equal quantities for each group. cDNA sequencing libraries were generated using NEBNext Ultra RNA Library preparation Kit for Illumina.