GSM4783665: Gba+/+_4; Mus musculus; RNA-Seq
Brain tissue was homogenized using a GentleMACS dissociator and mRNA was isolated using the RNeasy mini kit. RNA concentration (260/230 and 260/280 nm ratios)  were measured using a NanoDrop ND-1000 (Thermo Scientific, Waltham, MA, USA). RNA integrity was evaluated using an RNA screen tape on a Tapestation 2200 (Agilent, Santa Clara, California, USA). A bulk variation of MARSseq was used to construct RNAseq libraries: Jaitin DA, Kenigsberg E, Keren-Shaul H, Elefant N, Paul F, Zaretsky I, et al. Massively Parallel Single-Cell RNA-Seq for Marker-Free Decomposition of Tissues into Cell Types. Science. American Association for the Advancement of Science; 2014;343:776–9.