Full Length Non-Concatemer Reads: Cupressus sempervirens immature strobiles
The Iso-Seq SMRTbell library was constructed according to the standard isoform sequencing protocol  Procedure and Checklist- Iso-Seq  Express Template Preparation for Sequel and Sequel II Systems ,  barcoded and sequenced. The sequencing run was processed to generate the ccs reads and then the lima and isoseq3_refine procedures were used to remove primers, polyA tail, unwanted barcodes combinations and chimeric reads. The resulting submitted sequences correspond to full length non concatemer reads