GSM5656100: Control_3 (PFAS control); Danio rerio; RNA-Seq
10 embryos were pooled per replicate, ethanized via ice bath, homogenized in RNA-zol, and stored at -80°C until processing. Samples were then extracted using a Zymo Research Direct-zol RNA MiniPrep kit. Libraries were prepared for sequencing using standard BGI protocols for sequencing on the BGISEQ-500 platform. Briefly, mRNA was purified using oligo(dT)-attached magnetic beads and fragmented, cDNA was synthesized, subjected to end-repair and 3' adenylated, adapters were ligated to cDNA fragments, and then fragments were amplified and purified. PCR products were circularized and formatted into the final library, which was amplified with phi29 to form DNA nanoballs to be loaded onto the patterned array and sequenced.