GSM5656871: KBD1; Homo sapiens; RNA-Seq
Total RNA was extracted using the TRIzol reagent (Invitrogen, Carlsbad, CA, USA).The purity and concentration of RNA were measured using a NanoDrop spectrophotometer (Thermo Scientific, USA). Samples with OD A260/A280 > 1.8 were qualified for subsequent experiments Library preparations were sequenced on the Illumina Hiseq2500 platform