Amplicon sequencing of plankton assoiated bacteria assemblages: seawater biomass filters
Seawater plankton community biomass filters extracted for DNA and amplified for bacteria assemblages using the V4 region of the 16S rDNA with Illumina MiSeq adapters attached to primers. Samples were multiplexed using two unique sets of Illumina indeces on the same 2x250 bp Illumina MiSeq run at the Rhode Island Genomics & Sequencing Center. These reads have been demultiplexed, the Illumina MiSeq Nextera adapter sequences removed, and reads were separated by primer sequence and the the primer sequence was removed. The forward primer used was 515F: GTGYCAGCMGCCGCGGTAA and the reverse primer was 806R: GGACTACNVGGGTWTCTAAT (Apprill et al 2015, Parada et al 2016).Primers are reported from 5' to 3'.