GSM4736423: DNFB_rep4: RNAseq T cells; Mus musculus; RNA-Seq
Single cell suspension of flank epidermis were prepared by digestion with 0.3% trypsin for 50-60 minutes.  LiveCD45+Thy1.2-Thy1.1+ OT-I Trm were double FACS sorted to ensure high purity and directly lysed using the Clontech SMART-Seq v.4 kit for complimentary DNA synthesis. 200-1000 OT-I cells were collected in 12.5 uL of the lysis buffer. Illumina single and paired end libraries, RNA-seq and DNA-seq Adapter ligation to fragements followed by pcr amplification using indexed primers for Illumnia HiSeq-2500