GSM5114312: WGBS_HCC1954-GATA3KO_1; Homo sapiens; Bisulfite-Seq
One hundred nanograms of genomic DNA were fragmented to 350 bp using a Covaris E220 sonicator. DNA was bisulfite converted with the EZ DNA Methylation-Gold kit (Zymo Research). WGBS libraries were prepared using the Accel-NGS Methyl-Seq DNA Library Kit (Swift Biosciences) according to the manufacturer's instructions with six or seven PCR cycles for the final amplification. The libraries were purified using Ampure XP beads (Beckman Coulter).