GSM5737652: GKAO115_4d_KO_RNAseq; Mus musculus; RNA-Seq
Oocytes were collected by manual dissection in M2 medium RNA-seq libraries were prepared as described (Hanna et al. 2019  doi: 10.1186/s13059-019-1833-x) ChIP-seq libraries were prepared using ULI-nChIP, as previously described (Hanna et al. 2018 doi: 10.1038/s41594-017-0013-5). Antibodies were added using 250ng/reaction for both anti-H3K9me2 (mouse monoclonal, Abcam, ab1220) and anti-IgG (rabbit polyclonal, Diagenode, EB-070-010). Library preparation was completed with a MicroPlex Library Preparation kit v2 (Diagenode) with Sanger eight-base indices for multiplexing. Relative enrichment over input was quantified using the library concentrations determined by Bioanalyzer High Sensitivity DNA Analysis (Agilent). Low input bisulphite (BS)-seq libraries were prepared by post-bisulphite adapter tagging as previously described (Hanna et al. 2018 doi: 10.1038/s41594-017-0013-5)