WGS seq of canis lupus familiaris: adult female mammary tumor
We used the sequencing platform Novaseq6000 v1.0 reagent and PE150 (paired-end) with Forward sequencing strategy. For the library preparation, the in-house kit Novogene NGS DNA Library Prep Set (Cat No.PT004) for 350 bp insert DNA library was used. DNA was broken into pieces (average 350bp size) and then the adaptors (with primer and indexes) were added on double-strand DNA fragments.