GSM4736648: Male6-Brd4-rep2; Mus musculus; OTHER
The puromycin resistant cells were harvested and processed by transposon calling card protocol as described 'Self-Reporting Transposons Enable Simultaneous Readout of Gene Expression and Transcription Factor Binding in Single Cells'. The Calling Card library was prepared by assigning each well a unique Illumina P7 index, so that each well of a sample could be demultiplexed as a set of unique barcoded insertions. All 4 sets of libraries, Male6-WT (Male_PB_helper), Male6-Brd4 (Male_PB_Brd4_fusion), Female6-WT (Female_PB_helper), and Female6-Brd4 (Female_PB_Brd4_fusion), were then pooled and sequenced on an Illumina HiSeq 300.