Amplicon sequencing to assess the influence of plant age on the creeping bentgrass microbiome
Samples processed with extraction kits. Extracted DNA was PCR amplified to target bacterial (16S) and fungal (ITS) organisms. PCR products were purified, pooled per sample, and indexed with Nextera v2 indices. Indexed libraries were purified again, quantified, and sequenced as 2 x 300 paired end reads on an Illumina MiSeq.