GSM5235247: Testis rep1; Macrobrachium rosenbergii; RNA-Seq
Each shrimp was put on ice 1 min for anesthetizing, firstly. After anesthetizing, samples of ovaries (Ov), testicles (Tt) and androgenic gland (Ag) were collected separately and dissected. Each fresh sample was washed in phosphate-buffered saline (PBS; Gibco, Fisher Scientific, Waltham, MA, USA) and immediately frozen in liquid nitrogen. Total RNA was extracted from each sample using an RNeasy Plus Micro Kit (QIGEN Group, Germany) Total RNA was used to generate sequencing libraries with varied index label using a NEBNextUltra™ Directional RNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, MA, USA) according to the manufacturer's instructions