RNA-Seq of Rattus norvegicus: A7R5 cells
Confluent A7R5 cells were preincubated with DeMan, Rogosa, and Sharpe (MRS) broth at the ratio of 3% v/v for 12h, and then stimulated with 110-7 M angiotensin II for 4h. After angiotensin II treatment cells were collected and total intracellular RNA was extracted, reverse transcripted, and sequenced.