GSM5655377: IVT_IP_3; Drosophila melanogaster; RNA-Seq
The IVT RNA extract mixture was extracted two times with 20 uL of MyONE Streptavidin T1 (Invitrogen) for 15 minutes at 25 °C. Beads were washed twice and eluted using 2 mM avidin (IBA) at 25 °C for 15 minutes. Thereafter the RNA was isolated using TRIzol. Ribo-Zero Gold rRNA Removal Kit followed by Illumina TruSeq Stranded Library Prep