GSM5589675: Postnatal day 7 myeloid cells; Mus musculus; RNA-Seq
Brains were extracted and placed on ice in HBSS with HEPES buffer. Corpus callosi were dissected out and homogenized in glass dounces and filtered. Following centrifugation, cells were resuspended in 10% fetal bovine serum and run through a Percoll gradient. Cells were collected in FACS buffer, spun, and resuspended in HBSS. Myeloid cells were sorted based on expression of Cd11b.