ITS microbial communities in a textile wastewater photobioreactor
ITS NGS analysis was performed at Stab Vida Lda (Lisbon, Portugal). Sequencing targeted the ITS2 region of the eukaryotic rRNA genes with  ITS3_KYO2 (GATGAAGAACGYAGYRAA) (Toju et al. 2012) and ITS4 (TCCTCCGCTTATTGATATGC) (White et al., 1990) primers producing an amplicon length of 300-400 bp.  Samples were then sequenced with MiSeq Reagent Kit Nano though the Illumina MiSeq platform.