DMS of human ACE2, unsorted
An ACE2 gene library was generated via error-prone PCR, cloned into a yeast display vector, and transformed into E coli. This transformed library was then grown in a liquid culture, the plasmid DNA was extracted, the ACE2 gene insert was digested out of the yeast display vector, and isolated via gel extraction. The ACE2 gene insert was then randomly fragmented using the Nextera XT kit and sequenced using a 2x300 MiSeq v3 kit.