the whole genome bisulfite sequecing of Cynoglossus semilaevis: male muscle
Genomic DNAs were fragmented into 100-300 bp by Sonication (Covaris, Massachusetts, USA) and purified with MiniElute PCR Purification Kit (QIAGEN, MD, USA). The fragmented DNAs were then ligated to methylated sequencing adapters after been end repaired and A nucleotide added. The bisulfite conversion was subsequently conducted using Methylation-Gold kit (ZYMO, CA, USA) to convert unmethylated cytosine to uracil. Finally, the converted DNA fragments were PCR amplified and sequenced using Illumina HiSeqTM 2500 by Gene Denovo Biotechnology Co. (Guangzhou, China)