GSM5399377: Lrig1-high cells from normal colonic epithelium_S14; Mus musculus; RNA-Seq
RNA was extracted using the PicoPure RNA isolation kit (ThermoFisher) Libraries were prepared using SMARTer Stranded Total RNA-Seq Pico Input Mammalian v2 Kit (Takara) for a maximum of 8 cycles according to the manufacturers recommendations. Non-coding RNA species (rRNA and tRNA) were excluded using specific probes during the integral library preparation step. Paired-end RNA sequencing was performed with NextSeq sequencer using NextSeq 500 High Output v2 Kit (75 cycles) (Illumina).