bacterial community
The genomic DNA of composting was extracted by CTAB method, the DNA purity and concentration were performed by using agarose gel electrophoresis. Through 16S V4-V5 region primers (515F and 907R) that in the light of the instruction with China Beijing Novogene company carried on PCR amplification. The PCR product was detected by 2% agarose gel electrophoresis and depuration followed by Thermo Scientific. Clean sequences was obtained from Raw reads that through cutting, splitting, preliminary quality control at Cut-adapt (V1.9.1) and via UCHIME Algorithm compare with the species annotation database to detect chimeric sequences. Clustering and filter clean sequences into OTUs (Operational Taxonomic Units) by Uparse v7.0.1001, and performed species annotation as well as analysis from SSUrRNA database to obtained taxonomic information. MUSCLE (Version 3.8.69) was used for fast multiple sequence alignment to get phylogenetic relationship of all OTUs representative sequences.