Pacbio sequel II IsoSeq data using RNA-pool of adult tissues and developmental stages, grey mullet
The total RNA of 9 tissues and 5 developmental stages were pooled in equimolar concentration and subjected to cDNA synthesis and amplification using the NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module (NEB Inc., France) in conjunction with Iso-Seq Express Oligo Kit (Pacific Biosciences, USA). The library was constructed using the SMRTbell Express template Preparation Kit 2.0 (Pacific Biosciences, USA) as per manufacturers protocol. The library was purified using Pronex beads (Promega, US) and sequenced in 8M SMRT cell using PacBio Sequel II system in CCS/HiFi mode.