MinION metagenome: Allen Bay ice metagenome
Four MinION sequencing runs were performed using the extracts produced as described above in situ at PCSP. Three of the extracts (the filtered and unpurified extract, one purified extract, and the Claremont-produced extract) were prepared for sequencing using the Rapid PCR Barcoding kit (SQK-RPB004) and sequenced on separate R9.4 FLO-MIN106 flow cells with a MinION Mk1b device. One purified extract was prepared for sequencing with the VolTRAX sequencing kit (VSK-VSK002) and VolTRAX V2 device. It was subsequently sequenced on a R9.4 FLO-MIN106 flow cell with a MinION Mk1b device.