DMS of human ACE2, sorted
An ACE2 library was displayed on the surface of yeast and bound to soluble SARS-CoV-2 spike RBD protein. Fluorescence activated cell sorting was used to isolate ACE2 variants that bind to the spike protein. The plasmids for the spike-binding ACE2 variants were amplified in E. coli, the ACE2 gene insert was digested out of the expression vector, and isolated via gel extraction. The ACE2 gene insert was then randomly fragmented using the Nextera XT kit and sequenced using a 2x300 MiSeq v3 kit.