NextSeq 550 paired end sequencing; RNA-seq of human breast cancer cell line T47D and 2 different T47D_H2A.J-KO cells lines treated with tamoxifen and untreated controls
RNA-seq of human breast cancer cell line T47D and 2 different T47D_H2A.J-KO cells lines treated with tamoxifen and untreated controls
Cells were trypsinized, neutralized with medium, washed once with PBS, and the cell pellet was resuspended in Machery-Nagel Nucleospin RNA Plus denaturing lysis buffer. T47D cells and H2A.J-KO derivatives were cultured in RPMI-1640 medium supplemented with GlutaMAX (Thermo-Fisher Scientific 61870036), 10% fetal bovine serum, and penicillin (50 U/ml) + streptomycin (0.2 mg/ml) (Sigma P0781) in a 5% CO2 incubator.  Cells treated with 5 ÂµM tamoxifen for 4 days or left untreated. Machery-Nagel Nucleospin RNA Plus kit. Illumina TruSeq stranded mRNA kit.