GSM5398571: Smad3 binding region in BT549-HA cells; Homo sapiens; ChIP-Seq
ChIP-seq of BT549 cells were performed using 10 μg of anti-Smad3 antibody (Isogaya et al, Cell Res 2011) or anti-IRF3 antibody (#655704, Biolegend, San Diego, CA, USA, lot number unavailable) as described previously (Koinuma D et al, Mol cell biol, 2009). The libraries were constructed as described (Murai et al, Cell Discov, 2015), using IonXpress Plus Fragment Library Kit (Thermo Fisher Scientific). Adaptor-ligated samples were amplified by 15 cycles of PCR and purified by E-Gel SizeSelect (Thermo Fisher Scientific).