10x Chromium linked read WGS of Sandy the Desert Dingo: skin biopsy
Genomic DNA was prepared from skin biopsy with supplemental RNase (Astral Scientific, Taren Point, Australia) and proteinase K (NEB, Ipswich, MA, USA) treatment, as per the manufacturer's instructions. Isolated gDNA was further purified using AMPure XP beads (Beckman Coulter, Brea, CA, USA) to eliminate sequencing inhibitors. 150ng of DNA was size selected by the Sage Science Pippin Pulse (0.75% KBB gel; 9hr 10-48kb (80 V) program; Invitrogen 1kb Extension DNA ladder). A 10X GEM library was barcoded from high-molecular-weight DNA according to the manufacturers recommended protocols (Chromium Genome Reagent Kits v2 User Guide). QC was performed using LabChip GX (PerkinElmer, MA, USA) and Qubit 2.0 Flurometer (Life Technologies, CA, USA). The library was run on a single lane of a v2 patterned flowcell. Paired-end sequencing with 150bp read length was performed using the Illumina HiSeq X within the Kinghorn Centre for Clinical Genomics at the Garvan Institute of Medical Research, Sydney, Australia.