single nuclear WGS of indirect flight muscles (IFM) of adult female Drosophila melanogaster
IFM nuclei were isolated from thoraces via FACS sorting for GFP positive cells in (UH3-Gal4>UAS-GFP-KASH) a pool of 60-80 female thoraces at day 5 (young) and day 60 (old). The nuclear DNA was amplified via multiple displacement amplification (MDA). Libraries were created using the NEB kit and sent to Novogene for WGS.