GSM5009010: Upp5_H2Aub_R1; Marchantia polymorpha; ChIP-Seq
300 mg 15d thalli of WT, Mpbmi1/1l, Mph2a;H2AK115R/K116R and Mph2a;H2AK119R mutants were harvested. ChIP was performed as described before (Villar and Köhler, 2010). The ChIP-seq experiments were done using two biological replicates per IP, per genotype. The antibodies used were anti-H3 (07-690, Merck Millipore, Burlington, MA, USA), anti-H2Aub (#8240S, Cell signalling technology, Danvers, MA) and anti-H3K27me3 (07-449, Merck Millipore, Burlington, MA, USA). Libraries were generated using the Ovation Ultralow Sytem V2 (NuGEN, Redwood city, CA, USA) according to manufacturer's instructions, and 150 bp paired-end sequencing was performed on the HiseqX platform at Novogene (Hong Kong).