GSM5009299: Islets_DP111_T2D; Homo sapiens; RNA-Seq
Pancreatic tissue was sectioned in a cryostat and mounted on UV pre-treated Zeiss MembraneSlide 1.0 PEN slides. Laser capture microdissection (LCM) was done with a Zeiss Palm MicroBeam system using autofluorescence to identify islets, as previously described30. RNA was isolated from approximately 20x6µm3 of islet tissue using the Arcturus PicoPure RNA Isolation Kit. Sequencing libraries were prepared from bulk RNA using the Illumina SmartSeq protocol.