GSM6122977: Retina 1 ATAC-seq; Homo sapiens; ATAC-seq
ATAC-seq was performed with fresh dissociated cells from five retinas. Tagmentation and library preparation were carried out as described (Buenrostro, 2015) using Nextera DNA Library Prep kit (FC-121-1030, Illumina, San Diego, CA). Dissociated cells were quantified using a hemocytometer, and 50,000 cells were tagmented in a reaction mix containing 5 µl Tn5, 25 µl TD buffer 2X, 20 µl nuclease-free water in a thermomix with 600rpm agitation. The DNA was purified using the “MinElute PCR Purification” kit (Qiagen, 28004) followed by a two-sided size selection using a 0.5 and 1.5 ratio of SPRIselect reagent (Beckman Coulter B233181). All the DNA was used to prepare the libraries. Libraries were sequenced pair-end using the HiSeq 2500 platform (Illumina, CA, USA) at a read length of 50 base pairs.