GSM6123020: early gallbladder cancer [T13]; Homo sapiens; RNA-Seq
The TransZol Up Plus RNA Kit (Cat#ER501-01, Trans, Beijing, China) was used for total RNA extraction according to the manufacturer's instructions. The purified total RNA was subjected to rRNA removal, fragmentation, first-strand cDNA synthesis, second-strand cDNA synthesis, end repair, 3'end addition, connector ligation, and enrichment to build a sequencing library following the experimental instructions