GSM6250545: ATACseq.Control.KC.ZT10.req3; Mus musculus; ATAC-seq
50,000 isolated KCs were resuspended in 50 μl of transposase mixture (25 μl of 2× TD buffer, 2.5ul of TDE1, 0.5 ul of 1% digitonin, and 22 ul of nuclease-free water) (15027865, Illumina). Transposition reactions were incubated at 37 °C for 30 min in an Eppendorf Thermo Mixer with agitation at 300 rpm. Transposed DNA was purified using a QIAGEN MinElute Reaction Cleanup kit (28204), and purified DNA was eluted in 10 ul of elution buffer (10 mM Tris-HCl, pH 8). Transposed fragments were amplified and purified as described previously (PMID: 25559105).