GSM4742735: p20040_s052_7250_d2; Macaca mulatta; RNA-Seq
RNA was extracted from PAXgene RNA Blood Tubes using the MagMAX for Stabilized Blood Tubes RNA Isolation Kit (ThermoFisher). RNA was extracted from BAL fluid stored in PAXgene RNA Blood Tubes using the PAXgene Blood RNA Kit IVD (Qiagen). Globin transcripts in the blood RNA were blocked prior to library prep using the FastSelect Globin Reagent (Qiagen). Libraries were prepared using the Clontech SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio) in combination with the NexteraXT DNA Library Preparation kit (Illumina). Libraries were validated by capillary electrophoresis on an Agilent 4200 TapeStation, pooled at equimolar concentrations, and sequenced on an Illumina NovaSeq6000 at 100SR, yielding 25 million reads per sample.