scRNA-Seq of RGD#002:Spleen
Live human CD45+ cells were purified from porcine splenic cells by BD influx sorter. Cells were collected in PBS with 0.04% BSA and immediately counted using a LUNA-FL Dual Fluorescence Cell Counter (BIO-RAD, USA), and loaded in the 10x-Genomics Chromium (10x Genomics, CA, USA).