GSM5113056: 541M_d42_old_cDNA190920_E3; Homo sapiens; RNA-Seq
Index sorting of haemagluttin (HA) specific B cells was performed with samples from days 0 and 42. B cells were negatively selected magnetically from cryopreserved peripheral blood mononuclear cells, after 1 hour rest at 37C. Individual HA specific B cells were isolated using FACS and fluorescently labelled recombinant haemagglutinin. Sorts were performed into 96 well plates. Single HA specific B cells were index sorted in RNA lysis buffer, and samples were processed using plate-based SMART-Seq (SMART-Seq HT kit, Takara-Clontech). cDNA libraries were generated using Nextera XT adaptors, following the Takara-Clontech HT kit instructions. After library creation, libraries were pooled for clean-up. scRNASeq (SMART-Seq) of haemagglutinin specific B cell flow sorted from PBMC