GSM4736614: atg9 12 hours light replicate 3; Arabidopsis thaliana; RNA-Seq
Total RNA was extracted using an adapted CTAB/chloroform/LiCl extraction protocol (Chang et al 1993 Plant Mol Biol Rep). Full details are available on protocols.io: dx.doi.org/10.17504/protocols.io.3f6gjre. Total RNA-sequencing libraries were prepared using the TruSeq Stranded Total RNA with Ribo-Zero Plant (Illumina, CA, USA) as per manufacterers instructions but with reaction volumes adjusted 1/3x.