GSM5233529: 2uM-R1-TERT; Mus musculus; OTHER
Cells  were immobilized on magnetic beads and incubated with antibodies, followed by ChiTag and tagmentation. Libraries were prepared according to instruction from the NovoNGS CUT&Tag 2.0 High-Sensitivity Kit (for Illumina) (Cat. # N259-YH01). Briefly, DNA was purified with MinElute columns (Qiagen 28006) and eluted in 21 μL of elution buffer and mixed with 2 μL each of 10 μM indexed i5 and i7 primers and 25 μL of NEBNext HiFi 2x PCR Master mix. Libraries were amplified with the recommended conditions by the Kit. Library DNA was purified with MinElute columns (Qiagen 28006). CUT&Tag libraries were quantified by Qubit and DNA fragment size was determined by Agilent Bioanalyzer, and pair-end sequencing was performed with an Illumina NovaSeq 6000 syste following the manufacturer's protocols.