Deep sequencing of CcdA Saturation mutagenesis library studied in its operonic context
The SSM Library of CcdA in its native operonic context was subjected to phenotypic screening in resistant (TOP10Gyr) and sensitive (TOP10) strains. The experiments were carried out in three biological replicates.The CcdA gene was amplified using PCR from these samples with sample specific identifiers and was then pooled and further subjected to deep sequencing.