GSM5825799: GB_minus_1; Mus musculus; RNA-Seq
EDTA/DTT epithelial extraction followed by sorting on MoFlo XDP cell sorter for DAPI-, singlets, EpCam+, IL-25+ (RFP+ cells) and IL-25- cells Sequencing libraries were generated using the Nextera XT library preparation kit with multiplexing primers according to manufacturer's protocol (Illumina). Library fragment size distributions were assessed using the Bioanalyzer 2100 and the DNA high-sensitivity chip (Agilent Technologies). Library sequence quality was assessed by sequencing single-end 50 bp reads using the Illumina MiSeq platform and pooled for high-throughput sequencing on the Illumina HiSeq 4000 by using equal numbers of uniquely mapped reads (Illumina)