D21_intm1a_2
Developing barley inflorescence tissues were enriched for double ridge (DR), triple mound and spikelet meristem (TM-SM) and stamen primordium (StP) stages and frozen in liquid nitrogen. Total mRNA was extracted using TRIzol reagent (Invitrogen). Contaminated genomic DNA was removed using a DNA-free kit (Ambion). The quality of the total mRNA was evaluated using a bio analyzer (Agilent). RNA libraries were prepared for sequencing using standard Illumina protocols at the University of Cologne genome center.