GSM4736536: D0 RNAdecay 1h rep2; Homo sapiens; OTHER
RNA was extracted by trizol, and mRNA was purifiied by Poly(A)Purist™ MAG Kit RNA secondary structure libraries were performed by published icSHAPE method. Ribosome profiling was performed by following illumina TruSeq Ribo Profile (mammalian) Library Prep kit (RPHMR12126). RNA sequence library was performed by following NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760L). RNA structure, RNA expression, RNA decay and translation efficiency libraries were performed for the same 4 time point. Then all the different data can be used to do correlation analysis with high confidence.