WGS of Actinomadura sp. 32-07
The genomic DNA of Actinomadura sp. 32-07 was extracted using Zymo Research Soil Microbe DNA Kit (Zymo Research, USA). Subsequently, the purity and quantity of the extracted genomic DNA were measured using NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and Qubit 2.0 fluorometer (Thermo Fisher Scientific, Waltham, MA, USA), respectively. A 20-kb template library was prepared using the BluePippin size-selection system and the full genome is sequenced using a Pacific Biosciences RS II platform with P5-C3 chemistry following the MagBead loading protocol (Pacific Biosciences, Menlo Park, CA).