protozoans_breakdown_TIME_120_Treatment
DNA for meta-barcoding analyses was extracted with the QIAGEN DNeasy Blood and Tissue Kit, following the manufacturers protocols. The 16S rRNA gene (V3-V4 region) from the DNA samples were amplified using the universal bacterial primers (Caporaso et al. 2011), 515F (5-GTGYCAGCMGCCGCGGTAA-3) and 806R (5-GGACTACNVGGGTWTCTAAT-3). Additionally, we targeted eukaryotic sequences amplifying the 18S rRNA gene using the primers 574*f (5-CGGTAAYTCCAGCTCYV-3) + 1132r - (5-CCGTCAATTHCTTYAART-3) based on Hugerth et al. (2014). Barcodes were added via PCR and the amplicons were then cleaned up using a bead-based kit (AMPure XP, Beckman Coulter, US), pooled and sequenced (2  250 bp) on the Illumina MiSeq platform (DAmore et al. 2016).