rRNA gene of bacteria: Antarctic cryoconite hole sediment
DNA was extracted with MoBio PowerWater DNA Extraction Kit according to directions. Extracted genomic DNA was amplified in triplicate using 16S (515f-806r primers, Caporaso et al., 2012) SSU ribosomal gene markers. Amplified DNA was pooled and normalized to equimolar concentrations using SequalPrep Normalization Plate Kits (Invitrogen Corp., Carlsbad, CA, United States), and sequenced using 2  250 bp chemistry) at the BioFrontiers Sequencing CoreFacility at the University of Colorado at Boulder.