GSM5656074: STAD-PKU-22-me; Homo sapiens; MeDIP-Seq
cfDNA was extracted from 1-3 mL plasma using QIAamp MinElute ccfDNA Kit End repair and A-tailing are done by Kapa Hyper Prep Kit. Adaptors are NEBNext Multiplex Oligos index. Methylated sequences are pull down and purified by MagMeDIP kit and Diagenode iPure Kit v2. MeDIP library is amplificated by Kapa HiFi Hotstart ReadyMix (Roche) in 14-16 cycles