GSM6171511: Control,  PBMCs treated with HFn-Cur 4; Homo sapiens; RNA-Seq
Samples were homogenized and total RNA was isolated by Trizol® reagent(Life Science Technologies, Italy) following the manufacturer's specifications. RNAs were quntified using a Nanodrop ND-100 Spectrophotometer (Nanodrop Technologies, Wilmington, USA) and a 2100 Bioanalyzer (Agilent RNA 6000 Nano Kit, Waldbronn, Germany); RNAs with a 260:280 ratio of ≥1.5 and an RNA ntegrity number of ≥8 were subjected to deep sequencing. Sequencing libraries were prepared by the Illumina TruSeq Stranded Total RNA kit (Illumina) using 500-ng total RNA (Illumina) and by the Lexogen SENSE Total RNA-Seq Library Prep Kit using 500-ng total RNA (Lexogen). Qualities of sequencing libraries were assessed with 4200 TapeStation with the DNA1000 reagnt kit. RNA processing has been carried out using Illumina NextSeq 500 Sequencing.