GSM6124267: delta-cyaA with 0.3mM cAMP rep2; Escherichia coli str. K-12 substr. MG1655; RNA-Seq
Total RNA was extracted using the TRIzol-chloroform extraction methods, followed by removal of genomic DNA contamination. To enrich for mRNA 16S and 23S rRNA were depleted using Ambion Microbe Express Riboclean-up kits(AM1905). Libraries for each sample were prepared using the NEB NextUltra directional RNA library prep kit followed by single end sequencing using Illumina HiSeq 2500 platform.