PDX 983
Total RNA sample was digested by DnaseI (NEB), purified by oligo-dT beads (Dynabeads mRNA purification kit, Invitrogen), then poly(A)-containing mRNA were fragmented into 200-250bp with Fragment buffer (Ambion). Double strand cDNA synthesis and (standard or strand-specific) sequencing libraries were prepared and validated following the sequencing providers RNA-Seq protocols