GSM4736468: LFG3 RNA-seq; Cynoglossus semilaevis; RNA-Seq
To increase biological reproducibility, for each tissue, two individuals from large females or small females were firstly combined into one sample, and three samples for each tissue were employed for the following experiment. Subsequently, total RNAs of 30 samples named as SFB-1~3, SFP-1~3, SFL-1~3, SFG-1~3, SFM-1~3, LFB-1~3, LFP-1~3, LFL-1~3, LFG-1~3, and LFM-1~3 were prepared by Trizol (Invitrogen, USA) and assessed by the Agilent 2100 RNA 6000 Nano kit (Agilent, USA). RNA libraries were prepared for sequencing using standard Illumina protocols