GSM5588974: MLS402-91_siDDIT3_R1; Homo sapiens; RNA-Seq
Total RNA was extracted with an RNeasy Plus Mini Kit (QIAGEN, #74134) and DNase treated. Illumina TruSeq Stranded kit was used for library preparation. Each sample was barcoded with an i5/i7 pair of TruSeq DNA/RNA UDI indices. The pooled library was sequenced in two lanes.