RNA-Seq of Mesocricetus auratus: brain
Libraries were prepared starting from 250-700 ng of total RNA with the Truseq Stranded mRNA library preparation kit (Illumina, San Diego, CA, USA), following manufacturers instructions. Each library was run on an Agilent 2100 Bioanalyzer using an Agilent High Sensitivity DNA kit to ensure the proper range of cDNA length distribution. Sequencing was performed on an Illumina NextSeq instrument with NextSeq 500/550 High Output Kit v2.5 (300 cycles) in paired-read [PE] mode (Illumina, San Diego, CA, USA)