Chilli leaf curl virus infected Bemisia tabaci
cDNA Library Preparation The mRNA sequencing libraries were prepared using TruSeq RNA Library Prep Kit v2 (Illumina) following the manufacturers instructions. Briefly, 800ng of total RNA was used as input for poly (A) mRNA enrichment followed by fragmentation and reverse transcription to generate cDNA. Adapter index primers with unique barcodes for each sample were ligated to fragmented double strand cDNA. Ampure beads were used to purify adapter ligated fragments and the purified product was amplified using Illumina primers to generate sequencing library. The libraries were quantified using Qubit DNA HS kit and library quality was checked on Agilent Bioanalyzer 2100 using DNA 1000 kit.