GSM6123680: 22_ChIP-seq_H3K27ac_SUIT2; Homo sapiens; ChIP-Seq
Lysates were prepared from 5-10 millon single cell dissociated of organoid cultures or monolayer cultures. Sonicated nuclei with Bioruptor were further proceeded to ChIP assay with apporiate antibodies. Libraries were prepared using the NEXTflex ChIP-seq kit (Cat# NOVA-5143-02; PerkinElmer) according to the manufacturer's instructions.