msGBS of Vitis vinifera: berry at veraison
Grapevine berry genomic DNA (200 ng) was digested with 8 U of HF-EcoRI and 8 U of MspI. Sequencing adapters were ligated by adding 0.1 pmol MspI adapters, 15 pmol of the EcoRI Y adapter, 200 U of T4 ligase. Sequencing libraries were generated by pooling 25 ng of ligation product. The libraries were amplified by PCR and DNA Fragments ranging between 200 and 350 bp in size were captured using the AMPure XP beads. Libraries were sequenced using Illumina HiSeq 150 bp pair-end.