GSM6122464: HCC1806, parental, scRNAseq; Homo sapiens; RNA-Seq
Cells were detached using trypsinization and washed with 10x wash buffer (PBS plus 0.04% BSA) twice using 300g centrifugation. After last wash, the cell numbers were counted, and viability was assessed. The final sample were at the cell density of 0.7-1.2 million/mL. About 5000 cells per sample were mixed with 10x Single Cell 3' v2 RT reagents and loaded onto one channel of 10X Chromium Controller (10x Genomics, PN-1000202). After GEM generation, the RNA-seq libraries were processed following 10x Single Cell 3' Library Construction protocol (10X Genomics, Chromium™ Single Cell 3' Library Construction Kit v2, PN-120237). Final libraries were sequenced by SeqMatic (44846 Osgood Rd, Fremont, CA 94539) using Illumina NavoSeq 6000 system, with 2x75 paired-end kits.