18S microbial communities in a textile wastewater photobioreactor
18S NGS analysis was performed at Stab Vida Lda (Lisbon, Portugal). Sequencing targeting the 18S rRNA gene V9 region using the 1380F (CCCTGCCHTTTGTACACAC) and 1510R (CCTTCYGCAGGTTCACCTAC) eukaryotic primers and producing an amplicon of 200 bp (Amaral-Zettler et al., 2009). Samples were then sequenced with MiSeq Reagent Kit Nano though the Illumina MiSeq platform.