Whole genome shotgun sequencing for Mansonella perstans and its Wolbachia endosymbiont
DNA from M. perstans microfilariae from blood sample of an individual subject was extracted using a Genomic DNA Tissue MicroPrep kit (Zymo Research, USA).The DNA was treated with NEBNext Microbiome DNA enrichment kit (New England Biolabs Inc., USA) to enrich for parasite DNA and reduce human DNA contamination.Illumina libraries were constructed using the NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs Inc., USA)