GSM6122549: TNFα-treated, 18h, biol rep4; Homo sapiens; RNA-Seq
Total RNA was extracted using NucleoSpin® RNA kit (MACHEREY-NAGEL GmbH & Co. KG, Düren, Germany) and quantified using NanoDrop 2000 (Thermo Fisher Scientific, Wilmington, DE) according to manufacturer's instructions. RNA sequencing libraries were prepared using the NEBNext Ultra II Directional kit (New England Biolabs Inc., Ipswich, MA) with the poly(A) mRNA magnetic isolation module according to the manufacturer's instructions. The prepared libraries were validated using the D1000 Screen Tape assay on an Agilent 2200 TapeStation system (Agilent Technologies, Santa Clara, CA) and quantified by Kapa qPCR Library Quantification kit for Illumina (Kapa Biosystems, Boston, MA).