GSM5172791: -G6h_3; Nitzschia laevis; RNA-Seq
Total RNA was extracted using the plant RNA extraction kit (TaKaRa, Tokyo, Japan), and contaminating DNA was removed with RNase-free DNase I. The assessment of RNA concentration and quality was performed by Nano-Drop (ND1000 UV–Vis spectrophotometer; NanoDrop Technologies) and Agilent 2100 Bio analyzer (Agilent Technologies, Inc.). Poly-A containing mRNA molecules were purified, fragmented and copied into cDNA, then subsequent ligation of the adapter was performed. The products were purified and enriched with PCR amplification. The PCR yield was quantified by QuantiFluor® dsDNA System, and samples were pooled together to form the final library. The pooled samples were subject to cluster generation and sequenced using an Illumina HiSeq.