GSM5589527: MNaseseq_WT; Mus musculus; MNase-Seq
Cells were lysed in cold homogenization buffer (10 mM Tris-HCl pH7.5, 4 mM MgCl2, 1 mM CaCl2, 1X PIC) and fragmented with MNase (Thermo Scientific™,  88216). MNase-seq library were built using KAPA HyperPrep kit according to manufacturer protocol and converted to DNBSEQ-G400 compatible library using MGIEasy Universal Library Conversion Kit (App-A) (MGI tech, 1000004155) following manufacture instructions.