short-read sequencing of Picochlorum sp. BPE23 Mutant4
Illumina paired-end sequencing of mut4 with 150 bp reads was performed by Novogene (Novogene, United Kingdom). For library preparation, DNA was randomly fragmented to a size of 350 nt, then end polished, A-tailed, ligated with illumina sequencing adapters, followed by PCR enrichment of P5 and P7 oligos. The PCR product was purified (AMPure XP system) and sequenced.