GSM5277007: K562-Nanopore; Homo sapiens; OTHER
HMW genomic DNA were extracted from 5m cells using phenol chloroform approach (PMID30933081) Libraries were prepared using the Rapid Sequencing Kit (SQK-RAD004, ONT) according to manufacturer's recommendation. Briefly 1200ng DNA was incubated with 2.5ul of FRA at 30°C for 1 min and 80°C 1 min. This is followed by an addition of 3ul of adaptor (RAP) to the reaction mix and incubated at 5 min  at room temperature. The libraries were sequenced on the flowcell R9.4.1 (FLO-MIN106, ONT) on a GridION (ONT) using the MinKNOW software for 48hr