WGS of an artificial diploid E.coli
Libraries for single-molecule real-time (SMRT) sequencing was constructed with an insert size of 10 kb using the SMRT bell TM Template kit, version 1.0. Briefly, the process were that fragment and concentrate DNArepair DNA damage and endsprepare blunt ligation reactionpurify SMRTbell Templates with 0.45X AMPure PB Beadssize-selection using the BluePippin System repair DNA damage after size-selection. At last, the library quality was assessed on the Qubit 2.0 Fluorometer (Thermo Scientific) and detected the insert fragment size by Agilent 2100(Agilent Technologies).