GSM4736393: Treat_N1; Canis lupus familiaris; RNA-Seq
PBMCs were stimulated at 1 × 106 cells/ mL in 12 well-plate (PC312-0100) 37 °C and 5% CO2 in RPMI-1640 growth medium supplemented with 2500 IU/mL IL-2 (PROLEUKIN® huIL-2 (aldesleukin)) and 50 uM 2-Mercaptoethanol (Cat. M3148-25ML, Sigma-Aldrich) respectively, cultured for 14 to 20 days. RNA libraries were prepared for sequencing using KAPA mRNA HyperPrep Kit Illumina® Platforms protocol