MtDNA-seq of musculus
After the DNA samples passed the test, the Covaris ultrasonic crushing instrument was used to randomly break the DNA samples, and then the whole library was prepared through the steps of terminal repair, adding A-tail, adding sequencing connector, purification, PCR amplification and so on.After the library construction was completed, Qubit 3.0 was used for preliminary quantification and the library was diluted. Then QSEP100 was used to detect the inserts size of the library. After the inserts met the expectations, Q-PCR method was used to accurately quantify the effective concentration of the library (effective concentration of the library &gt;3nM) to ensure the quality of the library.