NextSeq 550 paired end sequencing; Bulk ATAC-seq of mouse Plasmodium-specific CD4+ T cells
Bulk ATAC-seq of mouse Plasmodium-specific CD4+ T cells
Fast-ATAC sequencing was performed as previously described (Corces et al. 2016). Briefly, 5,000 viable PbTII cells were sorted by flow cytometry and pelleted by centrifugation. Supernatant was removed and 50 µl of transposase mixture (25 µl TD buffer (Illumina), 2.5 µl of TDE1 (Illumina), 0.5 µl of 1% digitonin (Promega), 22 µl nuclease-free water) added to the cells. Cells were then incubated at 37°C for 30 min at 300 rpm in an Eppendorf ThermoMixer. Transposed DNA was amplified and purified using a QIAGEN MinElute PCR Purification kit, according to the manufacturer's protocol.