population1
The interrupted samples are selected with Agencourt AMPure XP-Medium kit, so that the sample bands are concentrated around 200-300 bp. Perform end repair on the fragmented DNA, add A to the 3'end, connect the sequencing adapter, and perform PCR amplification on the ligated product. After denaturing the PCR product to single-stranded, prepare a circularization reaction system, mix thoroughly and react at a suitable temperature for a certain time to obtain a single-stranded circular product. After digesting the linear DNA molecules that have not been circularized, the final library is obtained. The Agilent 2100 Bioanalyzer (Agilent DNA 1000 Reagents) was used to detect the fragment size and concentration of the library, and the library qualified by the quality test was sequenced on the BGISEQ-500 platform.