Corylus colurna
The library generation was performed using TruSeq RNA Sample Preparation kit and sequenced using Illumina HiSeq4000 with 100 bp paired end chemistry by commercial service providers  following recommended protocols. Briefly, mRNA enrichment was done using oligo (dT) beads and utilized for cDNA synthesis employing random hexamers followed by the second strand synthesis. The cDNA was fragmented and purified, ligated to adapters and used for sequencing. The two libraries were barcoded and run in a single lane.