16s of Macaca fascicularis
The genomic DNA of the sample was extracted by CTAB or SDS method, and then the purity and concentration of the DNA were detected by agarose gel electrophoresis. Using the diluted genomic DNA as a template, according to the selection of the sequencing region, use specific primers with Barcode, Phusion High-Fidelity PCR Master Mix with GC Buffer from New England Biolabs, and high-efficiency and high-fidelity enzymes to carry out PCR to ensure amplification Efficiency and accuracy. Primer corresponding region: 16S V4 region primers (515F and 806R): identification of bacterial diversity; 18S V4 region primers (528F and 706R): identification of eukaryotic microbial diversity; ITS1 region primers (ITS5-1737F and ITS2-2043R): identification of fungal diversity; In addition, the amplified regions also include: 16S V3-V4/16S V4-V5/16SV5-V7; Archaeal 16S V4-V5/Archaeal 16S V8; 18S V9 and ITS2 regions.