HiSeq X Ten paired end sequencing; RNA-seq of mouse skeletal muscle samples
RNA-seq of mouse skeletal muscle samples
The mice were treated with rIL-6 4.5 pg mouse grow in different time mouse sample from different time Total RNA was extracted using Trizol LS. Cells were fractionated and cytoplasmic RNA was extracted using the Paris Kit (Life technologies) according to the manufacturer's instructions mRNA-Seq libraries (from polyA+ cytoplasmic RNA) were created according to the standard Illumina protocol.