Illumina NovaSeq 6000 paired end sequencing; Time course RNA-seq profiling of touch response in Brachypodium distachyon
Time course RNA-seq profiling of touch response in Brachypodium distachyon
Samples were collected at the specified time following touch treatment, which consisted of 5sec stimulus with a metal probe along the root length. Seedlings were grown for 6 d on vertical 1x MS agar plates, ph 7.5, without sucrose, at 28C in the dark RNA was extracted using QIAGEN RNeasy Plant Mini Kit Libraries were constructed at the Joint Genome Institute, please see associated publication for more information.