RNA-Seq of Acipenser Baerii
The RNA concentration and purity were detected by Nanodrop 2000. RNA integrity was detected by agar-gel electrophoresis. A-T base pairing was performed with ployA using magnetic beads with Oligo (dT) to isolate mRNA from total RNA. mRNA was randomly fragmented and cDNA was synthesized by adding reverse transcriptase and random hexamers. cDNA libraires were sequenced using Illumina HiSeq.