GSM5399474: cel-miR-67; Rattus norvegicus; RNA-Seq
Cardiomyocyte cultures were isolated by enzymatic dissociation of 1 day-old neonatal rat hearts. Neonatal cardiomyocytes were seeded on Primaria 10 cm dishes and one day later, cells were transfected with mimics (Life Technologies) of hsa-miR-106b-5p, hsa-miR-93-5p, hsa-miR-25-3p or cel-miR-67 as control (25mM) using (Lipofectamine RNAiMAX, Life Technologies) and 72 hrs later RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit was used with 2 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols