Illumina MiSeq of Geobacillus EC-3
Libraries were prepared using the Illumina TruSeq Nano DNA Library Preparation Kit on a Perkin Elmer Sciclone NGS robot following the manufacturer's protocols. Completed libraries were QC'd and quantified using a combination of Qubit dsDNA HS, Caliper LabChipGX HS DNA and Kapa Illumina Library Quantification qPCR assays. Libraries were pooled in equimolar quantities and loaded on an Illumina MiSeq standard v2 flow cell; sequencing was performed in a 2x250bp paired end format using a v2 500 cycle reagent cartridge. Base calling was done by Illumina Real Time Analysis (RTA) v1.18.64 and output of RTA was demultiplexed and converted to FastQ format with Illumina Bcl2fastq v1.8.4.