Illumina reads of Enterobacter cloacae CMCC(B)45301 genome
First, the extracted DNA was fragmented to a size of ~350 bp via sonication. Using the NEBNextUltra DNA Library Prep Kit for Illumina, the fragments were then end-repaired, A-tailed, and adaptor-ligated, followed by purification and PCR-amplification. The amplified products were subjected to initial quality determination by Qubit 2.0 and size detection by Agilent 2100. The size-qualified fragments were further qualified using real-time PCR. Finally, the qualified library was sequenced by the Illumina Novaseq platform.