GSM5589189: DS_CV_3: Down's syndrome chorionic villi DS_CV; Homo sapiens; RNA-Seq
Cells were trypsinized, washed with PBS and total RNA extraction was performed using total RNA Purification Plus Kit (Norgen). RNA concentration and integrity were assessed using the RNA 6000 Nano Kit (Agilent Technologies). cDNA libraries were prepared using the TruSeq stranded mRNA kit (Illumina)