metegenomic sequencing of human adult plasma
Concatemers generated using rolling circle amplification were digested using T7 endonuclease to debranch and generate fragments of varying sizes. The fragmented DNA was subjected to a magnetic bead-based size exclusion cleanup protocol to keep fragments size between 500-10000bp. Following the size based clean up, samples that had a concentration greater than 500ng were selected for library preparation using SQK-LSK109 kit with native barcoding EXP-NBD104.