GSM5657467: Pacing rep3; Mus musculus; RNA-Seq
Ventricles were removed, flash frozen in liquid nitrogen, and total RNA was harvested using Trizol reagent and genomic DNA eliminated with on-collumn digestion uing RNeasy reagents (QIAGEN) polyA RNA was enriched and stranded libraries were prepared for sequencing using Illumina's TruSeq Stranded mRNA sample preparation kit 151bp paired-end libraries with SBS chemistry were sequenced on the Illumina HiSeq 4000 sequencer.