RNA-seq of Schlumbergera truncata: 15H05_sty_1
Pistils (without ovaries) were collected and immediately submerged in 1.5 ml of RNAlater solution (Invitrogen). Submerged samples were held at room temperature for thirty minutes and then moved to a -80C freezer for storage. Approximately 100 mg of tissue was ground to a fine powder in 1.5 ml tubes submerged in liquid nitrogen. Total RNA was isolated using Total RNA Mini Kit (Plant kit; IBI Scientific, Cat. No. IB47341) following manufacturer's instructions. We assessed RNA concentration and purity with a NanoDrop Lite Spectrophotometer (Thermo Scientific). Sequencing libraries were prepared using the KAPA Stranded mRNA-Seq (Roche), and these libraries were sequenced on a single lane of Illumina HiSeq 4000 platform (paired-end 150 bp reads) at the Duke University Center for Genomic and Computational Biology.