GSM5656128: e12.5_mouse_distal_forelimb_Eed427_RNAseq; Mus musculus; ChIP-Seq
RNA was extracted from dissected proximal and distal limb buds from 3 independent pools of wild type limb buds (n=3) and from two independent pools of Eedc/- limb buds (n=2). The extraction was performed using the RNAeasy Plus mini kit. Ribosomal depleted RNA. Next, mRNA enrichment, library preparation and flow-cell preparation for sequencing were performed by the IRCM Molecular Biology Core Facility according to Illumina's recommendations. Sequencing was done on a HiSeq 2000 instrument with a paired-end 50 cycles protocol. Library and flow cells were prepared by the IRCM Molecular Biology Core Facility according to Illumina's recommendations and sequenced on Illumina Hiseq 2500 in a 50 cycles paired-end configuration.