DNA-Seq of Human
genomic DNA was amplified by PCR with primers targeting the majority of known structural variations, SNVs and indels in the HBA1, HBA2 and HBB genes. Barcoded adaptors were ligated to the PCR products to construct individual sequencing library. Then each library was quantified and pooled together by equal mass. After purification and quantification, the pooled library was converted to SMRT bell library with Sequel Binding and Internal Ctrl Kit 3.0 (Pacific Biosciences) and sequenced on the Sequel II platform (Pacific Biosciences) under CCS mode.