RNA-Seq of mus musculus:male lung
RNA was extracted from CLP or normal lung tissue. Bioanalyzer 2100 system was used to detect RNA purity. Ribo-off rRNA Depletion Kit and MGIEasy RNA Library Prep Kit (1000006385, MGI, China) were used as to generate sequencing libraries in terms of manufacturer's instructions. Then, after purifying the PCR products by the AMPureXP system, the library quality was analyzed (Agilent Bioanalyzer 2100 system). Finally, the index codes were clustered by Cluster Kit of HiSeq 4000 PE (Illumina, USA). Total library constructions and sequencing were implemented at BGI Wuhan.