16S rRNA sequencing of mus musculus: fecal samples
Fecal samples were isolated from mice that were treated with Vascepa for 7 days and were also obtained after 6 weeks of high fat diet feeding. Genomic DNA was extracted from fecal samples using the  NucleoSpin Soil (Cat# 740780.50, MACHEREY-NAGEL). The V4 hypervariable region of the 16S rRNA gene was amplified. The library was purified using Ampure XP beads and loaded on to the Illumina MiSeq for sequencing, according to manufacturer instructions (Illumina, San Diego, CA) using the V2 (150bp x 2) chemistry.