Hi-C sequencing of Manduca sexta
To combine contigs into chromosome sized scaffolds, we generated Hi-C libraries using 200mg of adult M. sexta head of the same animal. The tissue was ground with a mortar and pestle cooled by liquid nitrogen. We then followed the Phase Genomics Proximo HiC animal kit (Phase Genomics, Inc., Seattle, WA, USA) protocol. The library was sequenced using the Illumina NovaSeq6000 (Illumina, Inc., San Diego, CA, USA) with paired-end 150 (PE150) read layout.