GSM5917097: AML patient UPN05 at diagnosis; Homo sapiens; ATAC-seq
Sorted AML samples were lysed with digitonin and tagmented with an engineered Tn5 transposase, following a protocol optimized for blood cells. Tagmented DNA was purified and amplified with 10 cycles of PCR. Before the sequencing, fragments with a 1-5 kbp size range were removed by magnetic separation with AMPure XP beads.