GSM5115043: lthsc_young_rep2; Mus musculus; Bisulfite-Seq
LT-HSCs were sorted from lineage-depleted hematopoietic mononuclear cells (10,000 cells per replicate). Cells were cultured overnight without growth factors at 3% O2 and washed twice with PBS before genomic DNA was isolated using the QIAamp DNA Micro Kit (Qiagen, Germany) and polyacryl precipitation carrier (Molecular Research Center Inc., Cincinnati, OH, USA). Tagmentation-based whole-genome bisulfite sequencing (T-WGBS) was performed as published in (Wang et al., Nat. Prot., 2013). We used 8-30 ng genomic mouse DNA as input.