GSM6123768: HCT116 DICER AA rescue AQ-seq replicate 2; Homo sapiens; miRNA-Seq
Total RNA was isolated using TRIzol (Invitrogen). Total RNAs were subjected to AQ-seq (Kim et al., 2019) except that 100 µg of total RNAs were used for library construction, and that cDNAs were amplified with NEBNext Ultra II Q5 Master Mix (NEB). The libraries were sequenced on the NovaSeq 6000 platform.