GSM5962716: Bace1-fl/fl;5xFAD; Mus musculus; RNA-Seq
Microglia were isolated from mouse brains using adult brain dissociation kits (MiltenyiBiotec, cat.no. 130-107-677). Briefly, forebrain was dissociated into a single-cell suspension, and myelin, cell debris, and erythrocytes were removed subsequently and cells were immunolabelled with CD11b microbeads (cat. No. 130-093-634). The cell suspension was allowed to pass though the magnetic column and retained CD11b-positive cells from the column, which were flushed out and used for analysis. Microglia were loaded into capture plates (a droplet-based 10x Chromium controller) to perform single-cell partitioning and mRNA was barcoded and subsequently converted into cDNA.