WGS of human adult skeletal muscle
The quality of isolated genomic DNA was verified by following methods: 1) DNA degradation and contamination were monitored on 1% agarose gels. 2) DNA concentration was measured using Qubit DNA Assay Kit in Qubit 2.0 Flurometer (Life Technologies, CA, USA). A total amount of 1.0g DNA per sample was used as input material for the DNA library preparations using TruSeq(TM) DNA sample prep kit. Sequencing library is generated using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, England) and following manufactures recommendations and index codes were added to each sample. Briefly, the genomic DNA is randomly fragmented to a size of 350bp by Bioruptor, then DNA fragments are narrowly size selected with sample purification beads. The selected fragments are then end polished, A-tailed, and ligated with the full-length adapter. After these treatments, these fragments are filtered with beads again. At last, the library is analyzed for size distribution by Agilent2100 Bioanalyzer and quantified using real-time PCR.