DNBSEQ-G400 paired end sequencing of SAMD00245770
The concentration of a genomic DNA solution was measured by Synergy H1 (Bio Tek) and QuantiFluor dsDNA System (Promega). A genomic DNA was fractionalized with 500 bp. The whole-genome sequence library was constructed using MGIEasy Universal DNA Library Prep Set according to the manual. The library of high-throughput sequencing platforms was prepared using MGIEasy DNA Adapters-96 (Plate) kit. The concentration of the library was measured by Qubit and Qubit dsDNA HS assay kit. The quality of the library was confirmed by Fragment Analyzer and dsDNA 915 Reagent Kit (Advanced Analytical Technologies). Circularize DNA was prepared using MGI Circularization Kit according to the manual. DNA nano ball (DNB) was made using MGISEQ-2000RS High-throughput Sequencing Set. DNB (2x150 bp) was sequenced using DNBSEQ-G400.