RNA of Berberis koreana
PacBio Iso-Seq library preparation and sequencing was done in DNA Link utilizing the RS II stage with P6-C4 science (DNA Link, Seoul, South Korea). Size choice was performed through a BluePippin (Sage Science, Beverly, MA) to build a cDNA library of size > 4 kb. A similar portion of unselected cDNA and > 4 kb cDNA were combined to frame Iso-Seq library for SMRT sequencing