Wild type_cowpea_replicate_1
The cowpea genotypes were grown in soil-pots under greenhouse conditions maintained at a diurnal temperature regime of 27/ 20C, 16/8 hr light/dark photoperiod, 75% of relative humidity, and white light illumination of 110 mol/m-2s-1. The pots were watered regularly up to a soil capacity of 80%. One gram of fully expanded fresh leaves were sampled from 6-week-old plants and flash frozen on dry ice. 1 g total RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat# RS-122-1001) was used for the construction of mRNA sequencing libraries as per the standard protocol.