GSM5917178: HGC27_FN1_3'UTR overexpression_rep3; Homo sapiens; RNA-Seq
Total RNAs were extracted from samples using TRIzol (Invitrogen) following the methods by Chomczynski et al. (DOI:10.1006/abio.1987.9999). DNA digestion was carried out after RNA extraction by DNaseI. RNA quality was determined by examining A260/A280 with NanodropTM OneCspectrophotometer (Thermo Fisher Scientific Inc). RNA Integrity was confirmed by 1.5% agarose gel electrophoresis. Qualified RNAs were finally quantified by Qubit3.0 with QubitTM RNA Broad Range Assay kit (Life Technologies). RNA libraries were prepared for sequencing using standard Illumina protocols.