NextSeq 550 paired end sequencing; Investigating the effects of Ceylon cinnamon water extract and metformin on HepG2 cells for type 2 diabetes therapy
Investigating the effects of Ceylon cinnamon water extract and metformin on HepG2 cells for type 2 diabetes therapy
HepG2 cells were grown to full confluence in 6‐well plates and conditioned in fresh HepG2 media for 24 h.  Treatments were performed in triplicate wells, and triplicates of untreated controls were also included. After 24 h, RNA was isolated and sent for RNA sequencing at the Donnelly Sequencing Centre (University of Toronto). The library was prepared with NEBNext Ultra II Directional RNA-Seq + rRNA, depletion (H/RM) + Full QC, Library Prep Kit (Illumina) and sequenced with NextSeq, flex service, HO150c + final pool QC, single read.  A RNeasy Mini Kit (74104; Qiagen, Venlo, Limburg, Netherlands), DNA‐free Kit (AM1906; Life Technologies) were bought from Qiagen and Thermofisher respectively, for RNA extraction.  High-throughput gene expression analysis is now a standard laboratory procedure to assess transcriptomic-level cell response to stress or changes in environmental conditions. RNA sequencing at the Donnelly Sequencing Centre (University of Toronto). The library was prepared with NEBNext Ultra II Directional RNA-Seq + rRNA, depletion (H/RM) + Full QC, Library Prep Kit (Illumina) and sequenced with NextSeq, flex service, HO150c + final pool QC, single read.