WGS of Pseudomonas aeruginosa PA14: Urine
Genomic DNA of each of the thirty days-evolved populations was extracted by using the Gnome DNA kit (MP Biomedicals, Santa Ana, CA, USA). The DNA quality check and sequencing was performed by Macrogen. Pair-end libraries (2x150) were obtained by using Truseq DNA PCR-free system and sequenced with an Illumina NovaSeq6000 instrument. The average number of reads per sample stands for a coverage greater than 300x.