WGS of C. tropicalis
Genomic DNA was isolated by phenol-chloroform extraction followed by purification using the Genomic DNA Cleanup and Concentrate kit from ZymoResearch. Library preparation and sequencing was performed using the Low Input Transposase-Enabled (LITE) method, a custom Nextera-based protocol. Samples were sequenced on two lanes of an Illumina HiSeq 2500 to give 2x250bp paired-end reads.