GSM6122993: Youth pulp, scRNAseq; Homo sapiens; RNA-Seq
The tissue was extracted and transported to laboratory in 4℃ α-MEM with 10% penicillin-streptomycin solution immediately. Soft dental pulp tissue was  washed in Mg2+/Ca2+ free phosphate-buffered saline (PBS)  following enamel and dentin was broken by sterilized pliers in super clean bench. Subsequently, the pulp tissue was shred into small pieces (<1 mm3) and centrifuged. The precipitation was digested for 40 min in 3mg/ml Type 1 Collagenase at 37℃, under agitation at 300 rpm in oscillation box, and blew by pipette gun 8-10 times every 8-10 min. The digestion was stopped by 2% FBS  and then filtered through a 40 μm cell strainer. After centrifuged, the pellet was re-suspended in PBS including 2% FBS and re-sieved through a 40 μm cell strainer. RNA libraries were prepared with Chromlum Single cell 3' Reagent Kits v3 according to the manufacture's protocol