single cell RNA-seq of the GM22648 LCL cell line
Single cell separation and Illumina compatible sequencing libraries were processed with 10X Chromium Controller using Chromium Next GEM Chip G Single Cell Kit and Chromium Next GEM Single Cell 3' Kit v3.1 (10X Genomics) according to the manufacturers protocol. Briefly, after single cell separation double stranded cDNA were generated and amplified by 12 PCR cycles. Library construction were continued with fragmentation, end repair & A-tailing, adaptor ligation steps and finished with 14 PCR cycles of amplification. Library quality and fragment size distribution were checked using DNA high sensitivity chip on BioAnalyzer 2100 (Agilent Technologies). Libraries were sequenced on NextSeq 500 (Illumina, San Diego, CA) sequencer.