Microbe sample from SCQ1
Total DNA was extracted using CTAB method. DNA quality control was performed under several steps: (1) DNA degradation degree and potential contamination was monitored on 1% agarose gels. (2) DNA purity (OD260/OD280, OD260/OD230) was checked using the NanoPhotometer spectrophotometer (IMPLEN, CA, USA).DNA was fragmented to size of 50-1000 bp, then DNA fragments were end-polished, A-tailed, and ligated with the sequencing adaptor using NEBNext Ltra DNA Library Prep Kit for Illumina (NEB, USA). DNA fragments with length of 300-500 bp were enriched by PCR. At last, PCR products were purified using AMPure XP system (Beckman Coulter, Brea, CA, USA) and libraries were analysed for size distribution by 2100 Bioanalyzer (Agilent, Santa Clara, CA) and quantified using real-time PCR. Qualified library were sequenced on an Illumina Hiseq 4000 platform and 150bp paired-end reads were generated.