RNA-Seq of Bemisia tabaci MED: hemolymph
Library construction involved enriching mRNA using oligo(dT) beads and removal of rRNA using Ribo-Zero kit. Subsequently, mRNA was fragmented and followed by first- and second-strand cDNA synthesis. Finally, cDNA libraries were generated through adaptor ligation and PCR enrichment. Library QC consisted of three steps: testing library concentration using Qubit 2.0, testing the insert size using the Agilent 2100, and quantifying the library effective concentration using qPCR. Libraries that passed QC were sequenced on a NovaSeq 6000 Sequencing System using NovaSeq paired end 150 sequencing setting.