DNA-Seq of homo sapiens: male patient's tonsillar
To investigate the presence of genes mutations the Lymphoma Solution targeted panel (SOPHIA GENETICS, Switzerland), that covers 54 relevant genes associated with many B  and T cell lymphomas (193 kb) (Supplementary Table 1) was used on the DNA extracted from the microdissected germinal center. Library preparation was conducted by initial DNA enzymatic fragmentation, end repair, A-tailing and adapter ligation, according to the manufacturers protocol. Purified amplified libraries were captured by hybridization with the capture probes. Lastly, captured pools were amplified and sequenced on a MiniSeq platform (ILLUMINA, US; Paired-end 2  150 bp; high-output kit).