GSM5233617: Nas2Del_Het_1027; Mus musculus; RNA-Seq
The whole forebrain was removed and flash frozen on dry ice. RNA was extracted using Qiagen RNeasy miniprep kit. Sample quality was confirmed using a Nanodrop and QC analysis was performed using a bioanalyzer to obtain RIN values.  Samples with appropriate RIN values were then chosen such that we had three independent triplicate samples for Nas2Del+/- Het and wild type. RNA-seq libraries were prepared for sequencing using TruSeq strandard total RNA kit from illumina. The library pool was diluted and denatured according to the standard NextSeq protocol (Document # 15048776 v02). Sequencing was performed using the Illumina NextSeq500 (NextSeq control software v2.1.0 / Real Time Analysis v2.4.11) to generate paired-end 76 bp reads using a 150 cycle NextSeq500/550 High Output reagent Kit v2 (illumina, FC-404-2002). Sequencing was performed at the Institute for Molecular Bioscience Sequencing Facility (University of Queensland).