GSM4736398: Ppy-expressing cells: YFPpositive2; Mus musculus; RNA-Seq
Islet cells were obtained from two-month-old doxycycline-treated Ppy-rtTA; R26-YFP mice, FAC-sorted in a Moflo Astrios (Beckman Coutler) system. Sorted YFP+ and YFP- cells of Ppy-rtTA; R26-YFP were loaded separately for single-cell RNA-seq using the Chromium Controller (10x Genomics) following the Single Cell 3' Library kit v2 manufacture's protocol. RNA libraries were prepared for sequencing using standard Illumina protocols