GSM5655266: Smad3-KO neutrophils; Mus musculus; RNA-Seq
CD11b+Ly6G TANs were FACS-sorted from the single-cell digestion of s.c LLC tumor inoculated in C57BL6 (wild-type) and Smad3-deficient (Smad3-/- ) mice and submitted for cell encapsulation and library construction by Chromium controller with 5' expression kit (10x genomics). Single-cell sequencing RNA libraries were prepared for sequencing using standard 10x Genomics single-cell droplet generation protocols and next-generation sequencing Illumina protocols 10x single-cell RNA-Seq