GSM5009034: macroH2A12_epi 2_I; Homo sapiens; RNA-Seq
Total RNA was obtained from HUVEC cells CTL, macroH2A1.1 and macroH2A1.2 with TRIzol Reagent (ThermoFisher Scientific, Waltham, USA). Indexed libraries were prepared from 2 mg/ea RNA with the TruSeq Total Stranded RNA Sample Prep Kit (Illumina, Cambridge, UK) according to the manufacturer's instructions. Libraries were quantified using Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, USA). Libraries were prepared so that each index‐tagged sample was present in equimolar amounts with a final concentration of 2 nmol/L. l/L.