BGISEQ-500 sequencing; [10x] scRNA-seq of mouse Plasmodium-specific CD4+ T cells
[10x] scRNA-seq of mouse Plasmodium-specific CD4+ T cells
PbTII cells from the spleen were isolated by flow cytometry into a 1% BSA/PBS buffer. For D7 and D28 timepoints, five recipient mice were pooled for isolating splenic PbTII cells.  PbTII cells were then sorted as single-cells using the Chromium 10X Genomics platform. Approximately 8,000 cells were loaded per channel onto a Chromium controller (10x Genomics) for generation of gel bead-in-emulsions using 10x Genomics Single Cell 3' Reagent Kit v3. Sequencing libraries were prepared using Single Cell 3â€™ Reagent Kits v3 (10x Genomics) and then converted using the the MGIEasy Universal Library Conversion Kit (BGI).