Complete Genome Sequence of Arachnia rubra strain DSM 100122T, A Cultured Member of the Human Oral Microbiome
Genomic DNA was extracted using a MasterPure DNA isolation kit (Lucigen) with a modified protocol that included bead beating for cell lysis. Single molecule, real-time sequencing (SMRTseq) was carried out on a PacBio Sequel Instrument (Pacific Biosciences; Menlo Park, CA, USA) with V3 chemistry, following standard SMRTbell template preparation protocols for base modification detection (www.pacb.com). Genomic DNA (5-10 g) samples were sheared to an average size of 15 kbp via G-tube (Covaris; Woburn, MA, USA), end repaired and ligated to hairpin barcoded adapters prior to sequencing. Sequencing reads were processed using Pacific Biosciences SMRTlink pipeline Version 8 (https://www.pacb.com/support/software-downloads/) with Microbial Assembly under default parameters