GSM6284674: SFB PAR-Clip; Homo sapiens; RNA-Seq
Cells were incubated with 0.1 mM of 6SG for overnight followed by 0.4 Jcm2 365nm wavelength UV treatment.RNA were isolated with PAR-Clip by streptavidin resin and Flag resin. Library preparation and sequencing were finished by Geneseed Technology CO., Ltd., Guangzhou, China. The protocol is as follows: 1) RNA quality and RNA sample assessment; 2) RNAs were fragmented, add adapters and indexes; 3) PCR; 4)Separation and cyclization; 5) Accurate quantification; 6) Sequencing.