GSM5117470: RNA-Seq 1232_PB; Homo sapiens; RNA-Seq
50,000 CD138+CD38+PI- cells were FACS isolated directly into 600 uL of RLT lysis buffer with 1% B-ME (Sigma-Aldrich M6250) prior to vortexing 1 min at max speed and freezing in a dry ice ethanol mixture. RNA was isolated (Qiagen RNeasy Mini 74104) and quality assessed using an Agilent BioAnalyzer. Stranded mRNA-seq libraries were made using the mRNA HyperPrep kit (Kapa 08098115702) following the manufacturer's protocol and using custom short TruSeq compatible sequencing adapters (IDT) (see Supplementary Table 1 from Barwick et al. Clinical Cancer Research 2021).  Barcodes were added to each library using long PCR primers (see Supplementary Table 1 from Barwick et al. Clinical Cancer Research) during 8 cycles of PCR amplification.