WES of mouse: Met Recurrent
To isolate genomic DNA, tumors were digested with Proteinase K in Buffer ATL (Qiagen). Following digestion, cells were lysed by adding SDS (final concentration, 0.5%) and sonicating to shear DNA. Genomic DNA was then purified using phenol-chloroform extraction followed by isopropanol precipitation. DNA yield from all samples was greater than 10 mg (minimum 1.5x106 cells). This genomic DNA was used for barcode PCR, qPCR, and whole-exome sequencing. Whole-exome sequencing was performed using the Agilent SureSelect XT Mouse All Exon Capture Kit and sequenced using the Illumina HiSeq 4000 with 150 base pair paired-end reads by the Duke GCB Sequencing and Genomic Technologies Shared Resource (Durham, NC).