GSM6067631: 20210224_Mouse_Meninges_SJL_3pr_v3_1; Mus musculus; RNA-Seq
Prior to euthanasia, old and young EAE and naïve SJL/J mice were injected intravenously with 3µg of anti-CD45-PE (eBioscience) to label blood-derived and blood vessel-adjacent immune cells. Leptomeninges and cortices were dissected and single-cell suspensions were prepared as previously described. Cells from each individual mouse were stained using BioLegend TotalSeq B hashtags (Hashtags 1-4, CAT#s 155831, 155833, 155835, 155837) and an anti-PE oligonucleotide barcode (BioLegend, CAT#408113). Cells from each compartment (i.e. leptomeninges) were mixed in a 1:1 ratio, resuspended to a concentration of 1,300 cells/µL, and submitted for sequencing on the 10X Genomics platform using 5' chemistry at the Princess Margaret Genomics Centre in Toronto, ON, Canada.