GSM4743092: Col-0 - ace_rep1; Arabidopsis thaliana; RNA-Seq
RNA was isolated from Arabidopsis seedlings using RNeasy Plant Mini Kit (Qiagen) according to the manufacturer's recommendations. One μg of total RNA was taken for DNAse digestion using the PerfeCTa DNAse I (Quanta Biosciences). Oligo dT based mRNA enrichment was carried out. RNA fragment and reverse transcription was performed after which end repair, add A-tailing and bubble adaptor ligation was carried out. PCR amplification was followed by single strand separation and cyclization to from DNA nanoball synthesis. Sequencing on DNBseq platform was then performed.