ampD8_1_DNAseq
The ancestral E. coli BW25113 and two replicate wells of amp D8 were sent for bacterial whole-genome sequencing. Briefly, overnight cultures were grown from frozen stocks in LB broth and 1 mL was pelleted. Cell pellets were immediately frozen at -80C and sent to Quintara Biosciences (Cambridge, MA) for DNA extraction and sequencing. DNA was extracted using the Universal Genomic DNA Kit (CW Biosciences), then sequenced on a NovaSeq S4 flow cell, with 2x150 paired-end reads at 100X coverage.