Comparative genomics analysis of Bacillus velezensis LOH112 isolated from a nonagenarian provides insights into its biocontrol and probiotic traits
Genomic DNA of B. velezensis LOH112 was extracted using the Bacterial DNA kit (Omega Co.) according to the manufacturers protocols. The experimental process is performed in accordance with the standard protocol provided by Oxford Nanopore Technologies (ONT). The extracted DNA quality was evaluated using Qubit 2.0 Flurometer (Life Technologies, Carlsbad, CA, USA) and  Nanodrop (Thermo Fisher Scientific, Wilmington, USA) accordingly. After the samples were qualified, they were sequenced based on the Nanopore sequencing technology platform according to standard protocols. The Canu v1.5 software with default parameters was used to assemble the filtered 110,954 subreads with an average length of 16,784 bp. Finally, the second-generation data were used by Pilon software to further correct the errors of the assembled genome to obtain a more accurate genome.