GSM5121354: OM-10.1 -SP - NoStim 2; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNeasy kit (Qiagen; no. 74106)  RNA was DNase treated using the TURBO DNA-free kit (Invitrogen; no. AM1907).mRNA was selectively isolated from total RNA (500 ng) using poly-T oligonucleotides attached to magnetic beads according to the manufacturer's guidelines in the NEBNext poly(A) mRNA magnetic isolation module (catalog no. E7490; NEB, Ipswich, MA). The library preparation from the enriched mRNA was conducted according to NEBNext Ultra II directional RNA kit (catalog no. E7760; NEB, Ipswich, MA).