GSM6124696: HAP1 50 uM Hemin biol rep 1; Candida albicans; RNA-Seq
Total RNA was extracted using hot phenol. NEBNext Ultra II Directional RNA Library Prep Kit for Illumina, cat no. E7760, using 800ng total RNA as a starting material. mRNAs pull-down was performed using the Magnetic Isolation Module (NEB, cat no. E7490). After construction, the concentration of each library was measured using Qubit (Invitrogen) and the size was determined using the TapeStation 4200 with the High Sensitivity D1000 kit (cat no. 5067-5584). 