GSM5795879: MI_8wk; Mus musculus; RNA-Seq
Mice were euthanized and perfused with PBS supplemented with EDTA, and hearts were harvested. Infarcted tissue was dissected under a stereomicroscope, and digested with DMEM medium with collagenase-II (5 mg/ml, Invitrogen 17101-015) and DNase (1 mg/ml, Sigma D4527). Single cell suspension was prepared after filtering with a mesh strainer (100-mm pores). Cells were treated and analyzed following a manufacturer's V3 library protocol (10x Genomics) and single-cell RNA-seq.