GSM6067718: 23C10; Mus musculus; RNA-Seq
Full length cDNA was generated from single cell using SMART-Seq v4 Plus according to the manufacture protocol. The cDNA quality is determined by 5200 Agilent Fragment analyzer. For RNA‐Seq, SMART-Seq v4 Plus(c at#R400753) was used to generate template library for sequencing on illumina NextSeq550 platform according to the manufacture protocol. Single-read sequencing of the cDNA libraries with a read length of 150 was performed with NextSeq 500 Sequencing System using NextSeq 500/550 High Output v2.5 kit 150 cycles (cat#20024907).