GSM6125977: HK2_2_DMSO_2; Homo sapiens; RNA-Seq
The samples were heated at 65°C for 8 min to denature RNA. Then, the samples were incubated at 45°C for 60 min so that probes were annealed to total RNA and were paired with target mRNA. Biotinylated oligo (dT) and streptavidin-coated magnetic beads were used to capture mRNA so that the aligned probes could resist washing. After washing, the aligned probes were ligated by T4 ligase at 37°C for 60 min. Libraries were prepared by PCR amplification, products from a single sample were indexed by limited PCR amplification with a set of barcode primers.