16S rRNA-Gene-seq of Homo Sapiens: adult urine
DNA was isolated with a MagAttract PowerMicrobiome DNA/Ribonucleic acid (RNA) Kit (Qiagen) using an epMotion 5075 liquid handling system. The V4 region of the 16S rRNA gene was amplified from 1, 2 or 5l of undiluted DNA and sequenced as described previously using Next Generation Sequencing, except an Illumina MiSeq Reagent Nano Kit v2 (500-cycles) was used.