Single-cell analysis of somatic mutations in human bronchial epithelial cells in relation to aging and smoking: Sample Bulk1289
Bulk genomic DNA for respective single cell collections was isolated from proximal bronchial basal cells using DNeasy Blood & Tissue Kit (Qiagen) according to the manufacturer's protocol. The libraries were sequenced with 2 x 150 bp paired-end reads on Illumina HiSeq X Ten sequencing platforms by Novogene, Inc.