WXS of homo sapiens: breast
Fragments of 150-200 bp were ligated to Illumina's adapters and PCR-amplified. 250 ng of DNA library was mixed with hybridization buffer, blocking mixture, RNase block and 5 l of SureSelect all exon capture library, according to the standard Agilent SureSelect Target Enrichment protocol. Hybridization to the capture baits was conducted at 65C using heated thermal cycler lid option at 105C for 221 hours on PCR machine.