GSM5560926: MCA205_Tissue_WT1; Mus musculus; RNA-Seq
MCA205 tumor tissue were harvested on day  8 post-implanted, and MCA205 WT and SIX1-/- cells were were collected, flash frozen on dry ice. Total RNA was extracted using RNeasy Mini Kit (QIAGEN, cat#74104). ProtoScript II Reverse Transcriptase (New England BioLabs, cat#E7420L) was used with 0.6 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols