GSM5963736: Skin tumor, WT SI Tissue Rep 7; Mus musculus; RNA-Seq
Tissues were stored in RNAlater until further processing. Tissues were homogenized in RLT buffer from the RNeasy Mini Kit (Qiagen) containing beta- mercaptoethanol. RNA was extracted using Qiagen RNeasy RNA extraction kit following manufacturer's instructions. Libraries for cluster generation and DNA sequencing were prepared following a previously described method (Fisher et al. 2011) using TruSeq RNA sample prep kit v2 (Illumina) The libraries were run on Illumina Next Seq using the High Output 75 cycles kit (2x36 cycles, paired end reads, single index)