GSM4743259: bulk-ATACseq_CD34+ cells - 24h_donor3; Homo sapiens; ATAC-seq
A one-step gentle membrane permeabilization and DNA transposition was performed by adding 50µl transposition mixture (25 µL TD buffer 2X, 2,5µL of transposase TDE1 (Illumina), 0,5 µL digitonin 0,1% (Promega) and 22 µL water) to the cell pellets and by incubating at 37°C for 30 minutes under agitation. Libraries were prepared according "Fast -ATACseq" protocol described in "Corces et al, Nat Genet. 2016 Oct; 48(10): 1193–1203".