GSM5860300: INC_G004_D5; Homo sapiens; RNA-Seq
Total RNA was isolated from PAXgene blood tubes using PaxGene Blood miRNA kits according to the manufacturer's instructions (Qiagen). RNA quality was assessed by bioanalysis (Agilent), with all samples having RNA integrity numbers > 6.0. Total RNA concentrations were determined by Qubit® 2.0 Fluorometer (Life Technologies, Carlsbad, CA, USA). Libraries were prepared from 200ng total RNA using KAPA RNA Hyperprep with RiboErase (Roche) kits.