ChIP seq of Flp-In T-Rex-293 MTS BG4: induced mitoBG4 ddC Rep2 IGG
Pull down with Flag and IgG from cross-linked total genomic DNA shared to achieve shearing of mtDNA to 300-500bp fragment lenght. Antibody bound-DNA was isolated and purified following standard procedures. INPUT from sheared samples was collected for sequencing analysis. DNA was subjected to mechanical fragmentation to achieve the proper size for library preparation. The NEBNext Ultra II DNA Library Prep Kit (New Englang Biolab) was used for sequencing library preparation according to the manufacturers protocols.  The library was quantified with Qubit and real-time PCR and size distribution was detected with bioanalyzer.