RNA-Seq of S. aureus
For the sample preparation of transcriptome sequencing, the overnight cultures of strains RMSA24 were diluted respectively to an OD600 of approximately 0.05 in 4 mL of fresh TSB broth and incubated in culture tubes at 37 C for 12 h with shaking. Subsequently, 2 mL cultures were transferred into sterile 48 well-flat-bottom tissue culture plates with 50 ul per well and were collected 3 h after desiccation in a thermostatic drier at 37 C, and the remaining 2 mL of the culture was collected without desiccation treatment.