GSM5827282: MCT-PAH3_Vehicle-Nebulization; Rattus norvegicus; RNA-Seq
RNA was isolated from rat lungs using the miRNeasy mini kit (Qiagen) combined with on-column DNase digestion (DNase-Free DNase Set, Qiagen) to avoid contamination by genomic DNA. 4µg total RNA was used as input for VAHTS Stranded mRNA-seq Library preparation following manufacturer's protocol (Vazyme).