GSM6125879: S118A mut 1, E2, GRHL2, Rep 3 [s118a-1_EG-3]; Homo sapiens; ChIP-Seq
Cells were fixed for 10 minutes with 1.5% formaldehyde and quenched with 0.125M glycine. Nuclei were extracted from cell pellets and sonicated to shear DNA. DNA was immunoprecipitated using an anitbody against GRHL2 (Millipore Sigma, #HPA004820). DNA was purified after reverse-crosslinking using the Qiagen PCR purification kit (Qiagen, #28104). Libraries were constructed using the NEBNext Ultra II DNA Library Prep kit (#E7103) and NEBNext Multiplex Oligos for Illumina (#E6440) according to vendor instructions. Samples were sequenced by paired-end 150bp sequencing on an Illumina NovaSeq 6000 by the Next Generation Sequencing department at the UW-Madison Biotechnology Center.