GSM5608507: siNT_plus_siNT; Mus musculus; RNA-Seq
Total RNA was extracted using the Trizol reagent (Invitrogen). Contaminating DNA was removed by DNaseI (Ambion) treatment, and the RNA was further purified using the QIAGEN RNeasy Kit. The RNA-Seq libraries were prepared using Illumina TruSeq Stranded mRNA Library Prep Kit (Catalog #: RS-122-2101) as per manufacturer's instructions.