GSM5859223: RNA-Seq D6 GLI1 OE rep2; Mus musculus; RNA-Seq
RNA was harvested samples with a Nucleospin RNA extraction kit (Machery-Nagel 740955.250). TruSeq RNA Sample prep kit v2 (Illumina RS-122-2001) was used with 1 ug of total RNA for the construction of sequencing libraries. Libraries were quantitated on an Agilent Bio-Analyzer and pooled in equimolar amounts. Pooled libraries were sequenced on the HiSeq4000 in Rapid Run Mode following the manufacturer's protocols to generate stranded single-end reads of 50bp.