GSM5560841: RPE Doxorubicin; Mus musculus; RNA-Seq
After removing retina from dissected anterior eye cups, RPE/choroid/scleral complex tissues were dissociated using the papain dissociation system (Worthington, Lakewood, NJ, USA).  RPE/choroid/scleral complex tissues were triturated and collected the dissociated single RPE cell after centrifugation at 1200 RPM for 3 minutes. scRNA-seq libraries of collected live cells were prepared using the Chromium controller and Next Gem Single cell 5' Reagent v2 kits (10x Genomics) Chromium Next GEM Single Cell 5p RNA library v1.1 Single Cell RNA-Seq