Ion Torrent 5S sequencing of Hermetia illucens gut microbiome
The NEXTflex DNA-Seq Kit was used for library preparation. PCR Amplicons were end-repaired and purified using the Agencourt AMPure XP reagent. IonCode Barcode Adapters were blunt-end ligated to PCR products and resulting barcoded libraries were purified with the Agencourt AMPure XP reagent. Quantified libraries were diluted to a concentration of 10 pM, pooled in equimolar amounts and subjected to sequencing template preparation using the Ion 510, Ion 520 & Ion 530 Chef Kits.