GSM5011789: RNA-seq of DNTET1 ctrl rep4; Homo sapiens; RNA-Seq
Total RNAs were extracted from human 293FT cells and mouse livers tissues by using TransZol® Up Plus RNA Kit (ER501-01, TransGen) For total RNA-seq, DNA libraries were prepared by using the NEBNext Ultra II FS DNA Library Prep for Illumina, and sequencing by using the Illumina HiSeq X Ten (2×150) at CAS-MPG Partner Institute for Computational Biology Omics CoreIndexed RNA libraries were prepared by using the TruSeq Stranded Total RNA with Ribo-Zero Globin. RNA-seq libraries were sequenced on an Illumina HiSeq X Ten (2×150) at CAS-MPG Partner Institute for Computational Biology Omics Core, Shanghai, China.