Single-cell transcriptome: Gregarines
The guts of the host animals were dissected with fine-tipped forceps under a low magnification stereomicroscopes. Hand-drawn glass pipettes were used to collect individual trophozoites under an inverted microscope. Trophozoites were rinsed at least three times in filtered seawater and stored in 2 L of cell lysis buffer. cDNA was synthetized from a pool of 2-5 cells, using the Smart-Seq2 protocol.Sequencing libraries were prepared using the Illumina Nextera XT protocol, and sequenced on the Illumina MiSeq (2x250bp) sequencer.