GSM4744692: Nuclear-shMETTL16-2.ip; Homo sapiens; RIP-Seq
Total RNAs were extracted from the cells treated with 4sU and then the total RNAs were subjected to biotinylation. The nascent RNAs were isolated from  biotinylated RNA with uMACS microbeads. Nuclear RNAs were extracted from nuclear pellets with miRNeasy Mini Kit and nuclear poly(A) RNAs were enriched from nuclear RNAs with  oligo(dT) polystyrene beads accordingly.The anti-m6A antibody (202003, Synaptic Systems) was applied for m6A immunoprecipitation Both input and m6A IP samples were utilized for next-generation sequencing (NGS) on the Illumina HiSeq 2500. 