GSM6124962: MCF7, DMSO RNA-seq, rep1; Homo sapiens; RNA-Seq
Total RNA was extracted using FastPure Cell/Tissue Total RNA Isolation Mini Kit (Vazyme). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was prepared by end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection.