Cut&Run assay of homo sapiens:UT-7 cell line
Cut&Run libraries were prepared from 0.1 to 10 ng of double-stranded DNA using the MicroPlex Library Preparation kit v3 (C05010001, Diagenode, Seraing, Belgium), according to manufacturer's instructions with some modifications. Amplified libraries were purified and size-selected using Agencourt SPRIselect beads (Beckman Coulter) by applying the following ratio: volume of beads / volume of libraries = 1,4 / 1. Libraries were sequenced on an Hiseq 4000 sequencer as paired-end 100 base reads following Illuminas instructions.