GSM5939913: RNA-seq G401 ACBI1 rep1; Homo sapiens; RNA-Seq
For ATAC-seq, 75,000 cells were harvested and nuclei were treated with the Active Motif transposase included in the Active Motif ATAC-seq kit. All subsequent processing following the Active motif protocol, except final libraries were cleaned up using a PCR purification kit rather than SPRI beads. For RNA-seq, RNA was extracted using Trizol and the Direct-zol RNA mini prep kit by Zymo Research. For ATAC-seq libraries were made using the Active Motif ATAC-seq kit, which included index primers and all PCR reagents. For RNA-seq, libraries were made at Vanderbilt University Medical Center VANTAGE core using their standard methods with ribosomal RNA depletion.