Sanger sequencing of RT-PCR, cell line, replicate 3 forward
RT-PCR and Sanger sequencing were used to validate the FGFR2-G3BP2 fusion transcript. PDC and PDO were harvested, following by RNA extraction and cDNA synthesis. Subsequently, PCR was performed and the PCR products were run on 0.8% agarose gel. PCR products were then cut from the gel and extracted by QIAquick Gel Extraction Kit (QIAGEN, #28704).