GSM5116896: Neuron_WT1; Mus musculus; Bisulfite-Seq
NPCs were covered with DNA lysis buffer and harvested using a cell scratcher. Neurons were isolated by FACS based on incorporation of the dye NeuroFluor (Stem Cell Technologies). Genomic DNA was isolated using the QIAamp DNA Micro Kit (QIAGEN) following the manufacturer's manuals. RRBS libraries were prepared using the Premium RRBS Kit (Diagenode) and purified twice using Agencourt AMPure XP beads (Beckman Coulter; 1X bead volume). Quality and concentration of RRBS libraries were determined using the High Sensitivity NGS Fragment Analysis Kit (Advanced Analytical) and a fragment analyzer with capillary size of 33 cm (Advanced Analytical).