GSM5011755: 24mo; Mus musculus; miRNA-Seq
Macrophage mRNA was extracted using MirVana mRNA isolation kit (Life Technologies). >10ug RNA was used to create cDNA libraries. Poly(A) RNA was isolated to reduce the non-coding (ncRNA) copy number in the pool. The RNA was randomly fragmented and converted to cDNA using standard chemistries for sequencing on the Illumina GAII sequencing platform.