GSM5962547: V233_PD1; Mus musculus; RNA-Seq
Quantseq libraries were prepared with 250 ng RNA using QuantSEQ 3' mRNA-Seq Library Prep Kit FWD for Illumina (015.96, Lexogen) according to manufacturer's recommendations. The libraries were pooled and sequenced with a 50-base-pair single-end protocol on an Illumina HiSeq 2500 sequencer. RNAseq. RNA was extracted using RNeasy mini kit (Qiagen) and treated with DNaseI (Qiagen). RNA-seq (Quantseq)