GSM6067971: Mus musculus liver from mice in Long light, recieving a LFD diet, at timepoint ZT16 [0101_114]; Mus musculus; RNA-Seq
Trizol+ Qiagen Rneasy according to manufacturers instructions Messenger RNA sequencing libraries were prepared using the Illumina TruSeq Stranded mRNA protocol (Illumina). Poly-A containing mRNAs were purified by poly-T attached magnetic beads, fragmented, and cDNA was synthesized using SuperScript III Reverse Transcriptase (Thermo Fisher Scientific). cDNA was adenylated to prime for adapter ligation and after a clean-up using AMPure beads (Beckman coulter), DNA fragments were amplified using PCR followed by a final clean-up. Libraries were quality-controlled using a Bioanalyzer instrument (Agilent Technologies) and subjected to 52-bp paired-end sequencing on a NovaSeq6000 (Illumina).