GSM6068467: hcar1-4+/+ no infection rep2; Danio rerio; RNA-Seq
~40 2.5-3 dpf zebrafish for each sample, mRNA were isolated using Trizol reagent (Life technologies) according to manufacturer's protocol. After Quant-It quantification and quality control of Agilent BioAnalyzer, 500 ng of total RNA underwent polyA selection and TruSeq library preparation according to instruction provided by TruSeq Stranded mRNA Library Prep Kit (Illumina). Samples were barcoded and run on a HiSeq 2500 in a 50bp/50bp Paired end run, using the TruSeq SBS Kit v4 (Illumina). An average of 42 million paired reads was generated per sample. RNA libraries were prepared for sequencing using standard Illumina protocols. 50 bp pair-end barcode length generated an average of 42 million paired reads per sample and sequenced on Illumina Hi-Seq 2500 system (MSKCC Integrated Genomics Operation core facility).