GSM5525331: ciPS_C6_d13_batch2; Pan troglodytes; RNA-Seq
On the day of harvest, the cells were washed once with PBS and lysed with 350 µl RLT buffer with 1% mercaptoethanol (Thermo Fisher). RNA was extracted using the RNeasy mini kit (Qiagen) according to manufacturer's protocol. The quality and concentration of the RNA was analyzed using 2100 Bioanalyzer (RNA nano; Agilent) and Qubit (RNA HS assay kit). TruSeq RNA Library Prep kit v2 (Illumina)