Amplification of16SrRNA gene of marine bacteria from chemostat experiment to investigate degradation of permafrost
Bacterial DNA was extracted from filters using the Qiagen Power Soil kit. Amplification of the 16S V3-V4 regions was performed using the sequence specific regions (341F-805R) described in (Herlemann et al., 2011) using a two-step dual-index PCR approach specifically designed for Illumina instruments.