16s rRNA of mouse fecal metagenome
The 16S rRNA primer pair, 515F GTGYCAGCMGCCGCGGTAA/ 806R GGACTACNVGGGTWTCTAAT, was utilized to evaluate the microbial ecology of each sample on the NovaSeq with methods via the bTEFAP DNA analysis service. Each sample underwent a single-step 30 cycle PCR using HotStarTaq Plus Master Mix Kit (Qiagen, Valencia, CA) were used under the following conditions: 95C for 5 minutes, followed by 30 cycles of 95C for 30 seconds; 53C for 40 seconds and 72C for 1 minute; after which a final elongation step at 72C for 10 minutes was performed.  Following PCR, all amplicon products from different samples were mixed in equal concentrations and purified using SPRI beads.   Samples were sequenced utilizing the Illumina NovaSeq chemistry following manufacturers protocols