16 rRNA HTS of microbial consortium for biological methanation
Filtration of N and Phix sequences  Elimination of sequences lower than 100nt  Sequences reduction at 5 and 3 extremities to 20nt  Dereplication to generate unique sequences  Use of DADA2 package for error rates and ASVs determination  Chimeras filtration  Taxonomic assignment with IDTAXAfrom package DECIPHER with SILVA138 database  phyloseq object generation  plots generation and statistical analysis with rANOMALY package  nanopore data treatment with Nanoclust software with NCBI 16S rRNA database as reference  Prinseq for quality filtration: average reads 15 minimum, 0 N base, sequences trim at 1700pb maximum and length of 1000pb minimum