GSM5917955: FABP/LPS-treated donor 2; Homo sapiens; RNA-Seq
Total RNA was isolated with an RNA purification kit (Marcherey-Nagel) and was subjected to poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Strand-specific cDNA libraries were constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs). Sequencing was performed using NovaSeq6000 (Illumina) sequencer in PE150 mode.