GSM5918170: Total RNA PA-1 DGCR8 heterozygote replicate 3; Homo sapiens; RNA-Seq
RNA from either wildtype or DGCR8 heterozygous PA-1 cells was extracted from a 60% confluent T75 cell flask (Gibco) with the miRNeasy Mini Kit (Qiagen), following the protocol for Purification of Total RNA, Including Small RNAs, from Animal Cells was followed, including the additional on-column DNAse digestion. After extraction, ≥ 200 ng of total RNA was used for rRNA removal stranded library sequencing with DNBseq PE100 by BGI Genomics.