Illumina HiSeq 4000 paired end sequencing
Bacteria was cultured in M63 minimal medium supplemented with 2 mM E2 dissolved in bettacycledextrins up to mid exponential phase. Biomass was collected by centrifugation and the pellet was stored at -80 ï¿½ C. Total RNA was extracted with comercial kit.
Library Kit: Ribo-Zero rRNA Removal Kit (Bacteria), TruSeq RNA Sample Prep Kit v2. Library Protocol: Ribo-Zero User Guide, TruSeq RNA sample prep v2 Guide, Part # 15026495 Rev. F. Reagent: TruSeq 3000 4000 SBS Kit v3.  Library Size Check of PCR enriched fragments: Agilent Technologies 2100 Bioanalyzer using a DNA 1000 chip. Library Quanity Check:  qPCR according to the Illumina qPCR Quantification Protocol Guide. Sequencing Protocol: HiSeq 3000 4000 System  User Guide Document # 15066496 v04 HCS 3.3.52. Type of Sequencer: HiSeq 3000 4000. Sequencing Control Software: HCS v2.2