GSM5939859: WGBS_ALMOND_3; Prunus dulcis; Bisulfite-Seq
Genomic DNA was isolated using a sorbitol pre-wash (Inglis et al., 2018) followed by an adapted CTAB method (Doyle and Doyle, 1990). Total RNA was extrated using the Maxwell RSC Plant RNA Kit and the Maxwell RSC instrument (Promega Corporation, Madison, WI, USA). Genomic DNA library preparation was carried out (Alioto et al., 2020), including two bisulfite conversion rounds. RNA libraries were prepared using TruSeq Stranded mRNA protocol and were sequenced on Illumina platform NewxSeq 500 (2x150 bp, Paired-end).