GSM5116359: X6_H15; Mus musculus; RNA-Seq
Single cells from dissociated hippocampus tissue were sorted by fluorescence cell cytometry and collected into a 384-well plates pre-loaded with 2.3 µl lysis buffer (50 mM guanidine hydrochloride, 17.4 mM dNTPs, 2.2 µM SMART dT30VN primer). Smart-Seq2 libraries were generated using Tecan Freedom EVO and Nanodrop II (Bionex) systems.