GSM5009717: URPL decidua single-cell RNA Seq; Homo sapiens; RNA-Seq
Decidual tissues were chopped using scalpels into approximately 0.5-mm^3 cubes and enzymatically digested with tumor dissociation kit (Miltenyi Biotec, 130-095-929) using GentleMACS Dissociator (Miltenyi Biotec, 130-093-235) following the manufacturer's instructions. The supernatant was diluted with cold RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) and then passed through a 40-μm cell sieve (Corning, 431750). The flow-through was centrifuged and cells were resuspended in 5ml of red blood cell lysis buffer (ThermoFisher, A1049201) for 5 min. The cells were then centrifuged and resuspended in cold RPMI-1640 medium supplemented with 10% FBS. The 10x Genomics v2 libraries were prepared as per the manufacturer's instructions. Libraries were sequenced, aiming at a minimum coverage of 50,000 raw reads per cell on an Illumina NovaSeq 6000. Single-cell RNA Seq