GSM6068255: MCF_7_siBCAS2_E2_0_Rep_1_ER_ChIP_R1; Homo sapiens; ChIP-Seq
Cells were treated, cross-linked with 1% formadelhyde, quenched with 125 mM glycine, and harvested in PBS.  Crude chromatin fraction was then sheared by a Bioruptor sonicator (Diagenode) for 30 cycles (30 sec on, 30 sec off).  Fragment size was verified using agarose gel before 500 micrograms of chromatin was used for immunoprecipitation using 2 microgram of anti-ERalpha antibody (Millipore Sigma, 06-935).  Sample was then washed, and DNA decrosslinked before library preparation. ChIP'ed DNA by an antibody against estrogen receptor alpha (Millipore Sigma, #06-935), and was used to generate ChIP-seq libraries.  The final libraries were quantified and subjected to QC analyses (i.e., number of PCR cycles required to amplify each library, the final library yield, and the size distribution of final library DNA fragments) and sequenced using an Illumina HiSeq 2000. Libraries were sequenced using an Illumina HiSeq 2000.