GSM6065793: F2 HLF, TGF-β1, Monosome mRNA; Homo sapiens; RNA-Seq
Total RNAs or mRNAs from monosome & polysome fractions of HLF cells were extracted and purified with TRIzol reagents, chloroform and isopropanol. 2 µg of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using QuantSeq 3' mRNA-Seq FWD library prep kits for Illumina.