GSM5795877: wt- rep #2; Mus musculus; RNA-Seq
Total RNA was extracted from the indicated cell cultures using the RNeasy kit (QIAGEN). Then, RNA integrity was evaluated on Bioanalyzer (Agilent 2100 Bioanalyzer), requiring a minimal RNA integrity number (RIN) of 8.5. Libraries were prepared according to Illumina's instructions accompanying the TruSeq RNA Sample Preparation Kit v2 (cat # RS-122–2001).