GSM6067570: SKBR3_Con_rep2; Homo sapiens; RNA-Seq
Total RNA was extracted from control and CTTN overexpressed SKBR3 stable cell lines tumors using TRIzol® RNA Isolation Reagents (Life technologies, Carlsbad, CA). RNA integrity was confirmed by a bioanalyzer using an Agilent RNA 6000 Pico Kit (Agilent, Santa Clara, CA). The isolated total RNA was processed for preparing mRNA sequencing library using TruSeq stranded mRNA sample preparation kit (illumina, San Diego, CA) according to manufacturer's instruction. Simply, mRNAs were isolated from 400 ng total RNA by RNA purification bead using polyA capture, and followed by enzyme shearing. RNA libraries were prepared for sequencing using standard Illumina protocols