PMseq-DNA of skin Proteus infection
The extracted nucleic acids were digested and fragmented, end repaired, ligated with adapters and PCR reactions were used to construct the library. The Agilent 2100 Bioanalyzer quality control library was used for the fragment size of about 300 bp, and the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific Inc.) was used for quality control. The concentration of the DNA library is controlled, and the constructed library is pooled according to the detected concentration according to the same quality, and the pooled library is circularized to form a single-chain circular structure. DNB nanospheres were then generated by rolling circle replication (RCA). The prepared DNB nanospheres were loaded into the sequencing chip and sequenced using BGISEQ-50