GSM5848491: Feeder-free PSC, treated with PD173074; Homo sapiens; Bisulfite-Seq
Genomic DNA was isolated from ESCs with the DNeasy Blood & Tissue kit (Qiagen), fragmented into 200-400 bp by sonication (Covaris), and was processed for bisulfite modification using EZ DNA methylation Gold Kit (Zymo). The libraries were prepared using Scale Methyl-DNA Lib Prep Kit for Illumina (ABclonal).