GSM5051372: S19ID00873; Homo sapiens; RNA-Seq
Total RNA was isolated using the RNeasy Mini kit (QIAGEN, Hilden, Germany) accord-ing to the manufacturer's instructions. To remove residual genomic DNA, the RNA sam-ples were digested with DNAse I. The RNA concentration was assessed by fluorescence quantitation using Qubit 2.0 and the HS RNA assay kit (Thermo Fisher Scientific Inc., Waltham, MA, USA), the RNA purity by spectrophotometry using Nanodrop 2000 (Ther-mo Fisher Scientific Inc., Waltham, MA, USA) and the RNA integrity by electrophoresis using TapeStation 4200 RNA ScreenTape (Agilent Technologies, Santa Clara, CA, USA). Library samples were prepared with the TruSeq Stranded mRNA Library Prep kit (Illu-mina, San Diego, California, USA) as recommended by the manufacturer. Paired-end se-quencing (2 × 100 pb) was performed with NovaSeq 6000 system (Illumina, San Diego, CA, USA), with a minimum of 25 million reads per sample and read quality of 90% > Q30.