TCR V beta from peripheral blood of localized prostate cancer patient before Radiotherapy
The Oncomine TCR Beta LR Assay (Thermofisher) was adopted for profile analysis of the TCR V repertoire in blood samples of LPCa patients that were subjected to Radiotherapy.  Following template preparation and chip loading with the Ion Chef System, libraries were sequenced with the Ion GeneStudio S5 System using the Ion 510 & Ion 520 & Ion 530 Kit  Chef and the Ion 530 Chip Kit (Ion Torrent, Thermo Fisher Scientific). Subsequent immune repertoire analysis was performed using the Ion Reporter Software with the Oncomine TCR Beta-LR- Single Sample workflow (Ion Torrent, Thermo Fisher Scientific).