GSM4903988: hdc_ko_hypothalamus_8f_GCCGTTT; Mus musculus; RNA-Seq
Mouse brains rapidly dissected on ice.  Dissected tissues were ventral and dorsal striatum, hypothalamus, and motor cortex.  Tissue was rapidly frozen and stored at -80° until processing using the RNeasy MinElute columns (Qiagen, Valencia, CA). RNA quality and quantity were confirmed with a Bioanalyzer (Agilent Technologies, Santa Clara, CA).  We generated double-stranded complementary DNA using the Ovation RNA-Seq system V2 (NuGEN Technologies, Inc., San Carlos, CA), starting from 5 ng of RNA.  Standard sequencing libraries (Illumina, Inc., San Diego, CA) were generated from 1-2 µg of cDNA, sheared to ~200 bp.  Sequencing was performed at the Genome Technology Access Center (GTAC) at Washington University in St. Louis. Fastq files containing demultiplexed, single-end 50 base-pair reads were used as the basis for exploratory analysis.