GSM5562304: Calu 1331; Homo sapiens; RNA-Seq
Individual cells were captured in chambers of Fluidigm HT mRNA-seq IFC. Total RNA extraction and preamplification of synthesized cDNAs were performed in Fluidigm C1 systems using the script of 'mRNA Seq HT:RT & Amp (1912x)'. A total of 1,600 3'-end enriched scRNA-seq libraires (400 libraries per cell line) were constructed using Nextera XT index Kit v2 Set A/B and Nextera XT DNA library preparation kits. 3'-end enriched scRNA-seq using Fluidigm C1 system