GSM5115738: nTE_D2; Homo sapiens; RNA-Seq
Cultured cells were dissociated with Accutase. Single cells were collected using a BD FACSAria II (BD Biosciences). nTE_D2 and nTE_D3 samples were purified with TACSTD2 antibody. For scRNAseq, single cell suspensions of all samples were resuspended in PBS-1% BSA at 1000cells/μL and barcoded with a 10x Chromium Controller (10x Genomics). Sequencing libraries were constructed with reagents from a Chromium Single Cell 3' Reagent Kits v3.1 (10x Genomics). Sequencing was performed with Illumina HiSeq 4000.