NextSeq 500 sequencing; ChIP-seq for RUNX1 and ETV6-RUNX1 in B-ALL cell lines and patient samples
ChIP-seq for RUNX1 and ETV6-RUNX1 in B-ALL cell lines and patient samples
Patient samples were expanded in NSG mice. Following intra-tibia injection mice were monitored for human engraftment and upon reaching >90% hCD45 in the bone marrow were culled. Bone marrow cells were harvested for further processing.  Cell lines were cultured and harvested according to standard protocols  Cells were subjected to standard chromatin immunoprecipitation (ChIP) protocols.  Illumina Truseq