GSM6065739: 3x-FLAG-rtrC ChIP output replicate 2; Caulobacter vibrioides CB15; ChIP-Seq
Crosslinking was induced with 1% formaldehyde and quenched with 0.125 mM glycine. Cells were lysed and DNA was sheared by sonication. Samples were pre-cleared on SureBeads Protein A magnetic beads. 5% of total volume sample was removed as input. Samples were incubated on α-FLAG magnetic beads, then eluted with 100 ng/µl 3xFLAG peptide. Samples were subject to RNase & proteinase K treatment, then purified with the Zymo ChIP DNA clean & concentrator kit. Sample libraries were prepared using the Illumina DNA Prep kit and IDT 10bp UDI indices, and sequenced on an Illumina NextSeq 2000, producing 2x151bp reads. Demultiplexing, quality control and adapter trimming was performed with bcl-convert