Illumina paired-end DNA sequencing; Tylothais aculeata; muscle
For Illumina short reads sequencing, ~200 ng DNA per sample was used for the DNA library preparations. Sequencing library was generated using TruSeq Nano DNA HT Sample Prep Kit (Illumina USA) following manufacturers recommendations and index codes were added to each sample. Briefly, genomic DNA sample was fragmented by sonication at 350 bp. Then DNA fragments were end polished, size selected, A-tailed, and ligated with the full length adapter for Illumina sequencing, followed by further PCR amplification. After PCR products were purified (SPRIselect reagent, Beckman), libraries were analyzed for size distribution by Agilent 2100 Bioanalyzer and quantified by Qubit. The DNA libraries were sequenced on Illumina NovaSeq 6000 platform and 150 bp paired end reads were generated by Genomics BioSci & Tech Co.