Illumina HiSeq 2500 paired end sequencing; DNA sequencing comparing different stages of leukemic progression in a mouse model of severe congenital neutropenia (SCN)
DNA sequencing comparing different stages of leukemic progression in a mouse model of severe congenital neutropenia (SCN)
Upon sacrifice, bone marrow was isolated from femurs and tibias of the mice. Cells were harvested by flushing the bones and filtered. Subsequently, GFP+ Csf3r-d715/RUNX1-D171N cells were sorted for sequencing. 100 ng genomic DNA was fragmented using enzymatic fragmentation. Sample libraries were constructed following the SeqCap EZ HyperPlusCap workflow Userâ€™s Guide version 1.0 (Roche). Unique, dual index adapters (Integrated DNA technologies) were used for ligation. After ligation of adapters and an amplification step, exome target sequences were captured using in-solution oligonucleotide baits (SeqCap EZ Developer Library mm9_exome_L2R_D02).