18S sequences of Astragalus sinicus and Vicia villosa collected from China
We used the 18S rRNA V4 region of the ribosomal RNA gene cluster. This region was amplied with the forward primer 528F (GTGCCAGCMGCCGCGGTAA) and reverse primer 706R (GGACTACHVGGGTWTCTAAT). Sequencing libraries were generated using Ion plus Fragment Library Kit 48 rxns (Massachusetts, USA) following the manufacturers recommendations.