GSM5964184: CTL_1; Homo sapiens; RNA-Seq
Total RNA was isolated and purified from HUVEC using the RNA Mini Kit according to the manufacturer's protocol (Bio&SELL). Purified RNA was reverse transcribed with SuperScript III Reverse Transcriptase (Thermo Fisher) and oligo(dT)23 together with random hexamer primers (Sigma). RNA-sequencing was performed as described previously (Leisegang et al., 2019). Briefly, Total RNA and library integrity were verified and 500 ng of total RNA used as input for SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Clontech). Sequencing was performed on the NextSeq500 instrument (Illumina) using v2 chemistry, resulting in an average of 30M reads per library with 1x75bp single end setup. RNA-IP input samples were sequenced at 150M reads per library