GSM6171844: IPSC – Replicate 1 – Untreated – 24h; Homo sapiens; RNA-Seq
Total RNA was extracted from at least 200.000 cells collected at the end of dead cell removal step, using a RNeasy Plus mini kit according to the manufacturer's instructions (Germantown). Total RNA was eluted in 30 µL RNA-free water. Genomic DNA was removed by using DNAse I (Thermo Fisher Scientific) and the purified RNA was recovered using SurePrep RNA Cleanup and Concentration Kit, according to the manufacturer's instructions (Fisher Scientific). The genomic DNA removal and clean-up step was repeated a second time. The purity of the finally obtained total RNA was evaluated using a Bioanalyzer. RNA-seq was performed on a NovaSeq 6000  (Genomics Core, Indiana University School of Medicine).