RNA-Seq of homo sapiens: human CD4+ T cells
Peripheral blood mononuclear cells (PBMC) were collected, and CD4 T cells were isolated by immunomagnetic positive selection (EasySep Human CD4 positive selection kit; Stemcell Technologies). RNA was extracted from purified CD4 T cells by Trizol-Column (PureLink RNA Mini kit; Thermo Fisher Scientific). Whole transcriptome libraries were prepared with the Ion Torrent Total RNA-Seq Kit v2 (Thermo Fisher Scientific) from 200ng total poly-A enriched RNA (Dynabead mRNA direct Micro Kit; Ambion). Sequencing was done on an Ion P1 chip using the Thermo Fisher Ion Proton System to a minimum of 30M reads.