GSM4743625: WT old microglia 1; Mus musculus; RNA-Seq
microglia were flow cytometry sorted from three separate mice per replicate, pooled prior to RNA extraction, which was carried out using an RNAeasy microkit (Qiagen), according to manufacturers' guidelines. Samples were prepared for RNA sequencing using the SMARTer Ultra Low RNA kit for Illumina sequencing (Clontech Laboratories) according to the manufacturer's instructions.