Amplicons of 16S and ITS regions: soil samples
DNA libraries for all samples were prepared with the Swift Amplicon 16S+ITS Panel kit (Swift Bioscences), according to protocol, with 50 ng as starting material. Final library concentrations were measured with the Qubit BR dsDNA assay kit (Thermofisher) and used to prepared an equimolar pool. The poll was then quantified by Real-Time PCR against a standard curve with the KAPA Library Quantification Kit (Kapa Biosystems). Libraries were sequenced on a NovaSeq 6000 Illumina platform with a SP flow cell in a 250PE mode and about 1 to 2 million fragments were produced per sample