RNA seq of homo sapiens:UT-7 cell line
RNAseq libraries were prepared starting from 1 g of total RNA using the Universal Plus mRNA-Seq kit (Nugen) as recommended by the manufacturer (libraries can be prepared from 10 ng to 1g of starting total RNA). The oriented cDNA produced from the poly-A+ fraction were sequenced on a NovaSeq6000 from Illumina (Paired-End reads 100 bases + 100 bases). A total of ~30 millions of passing filter paired-end reads was produced per library.