GSM5939255: pDC pano H3K27Ac; Mus musculus; ChIP-Seq
Spleens were removed and dendritic cells sorted. Cells were fixed with 1% formaldehyde for 6min and sonicated using the S220 Focused-ultrasonicator (Covaris) for 8min. Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with H3K27Ac antibody (Abcam Cat# ab4729). Chromatin was isolated with the True Microchip Kit (Diagenode Cat# C01010130). Libraries were prepared using the DNA Library Prep NEB (New England Biolabs)