GSM4904036: Stalk_control_r1; Homo sapiens; RNA-Seq
Bulk RNA-Seq: Samples were dissociated and resuspended in HBBS + 2% FBS (50-100 ml per organoid). Where required, cells were stained for 30 min with Alexa Fluor 488 anti-EpCAM antibody (Biolegend) followed by three washes with HBBS + 2% FBS and resuspension in 2 ml HBBS + 2% prior to being sorted based on mCherry reporter expression and EpCAM-488. Following collection, the cells were pellet by centrifugation (3 min, 300 g) and RNA extracted using the Isolate II RNA Mini/Micro Kit (Bioline) Bulk RNA-seq: Libraries were prepared using the Illumina TruSeq Stranded Total RNA library Prep Kit. Sequenced using Illumina NextSeq 500