GSM5277195: Psoriatic skin from donor 5851 [PP-5851]; Homo sapiens; RNA-Seq
6-mm punch biopsies were taken from healthy skin from healthy donors, lesional and non-lesional skin from psoriasis patients. Samples were incubated overnight in 0.4% dispase (Life Technologies) in Hank's Balanced Saline Solution (Gibco) at 4°C. Epidermis and dermis were separated. Epidermis was digested in 0.25% Trypsin-EDTA (Gibco) with 10U/mL DNase I (Thermo Scientific) for 1 hour at 37°C, quenched with FBS (Atlanta Biologicals), and strained through a 70μM mesh. Dermis was minced, digested in 0.2% Collagenase II (Life Technologies) and 0.2% Collagenase V (Sigma) in plain medium for 1.5 hours at 37°C, and strained through a 70μM mesh. Epidermal and dermal cells were combined in 1:1 ratio and libraries were constructed by the University of Michigan Advanced Genomics Core on the 10X Chromium system.