GSM5917421: Fucci sample 2; Mus musculus; RNA-Seq
Single cells were sorted or transferred directly into cell lysis buffer. The reverse transcription and template switch steps were then performed following the modified Smart-seq2* protocol. Amplified cDNA was sonicated to ~300-base-pair (bp) fragments by the Covaris S2 system. The 3' ends of the transcripts are enriched by Dynabeads Myone streptavidin C1 beads (invitrogen) after biotin-primer PCR. The cDNA libraries were generated using a KAPA Hyper Prep Kit for Illumina (KAPA). Finally, the samples were ready for sequencing on an Illumina Hiseq platform (Novogene). Modified Smart-seq2