GSM5738034: FCH7HF7CCX2_L7_CHKPE85219100041 URE  /+;Tet2-/-; Mus musculus; ATAC-seq
Total bone marrow cells were isolated from the femurs, tibiae, pelvic bones and spines of mice and were treated with 1x ACK lysing buffer pH 7.4 to lyse the red blood cells. cKit+ Lymph- Ery- cells were  FACS-purified and gDNA was extracted using the Puregene Cell and Tissue kit (Qiagen) ATACseq library construction was performed as previously described (Corces et al 2017)