GSM5658200: MDA-LM2_rpf_r2; Homo sapiens; OTHER
The lysates for Ribo-seq were prepared in lysis buffer (20 mM Tris pH 7.6, 150 mM NaCl, 5 mM MgCl2, 1 mM DTT, 100 µg/mL cycloheximide, 1% v/v Triton X-100 and 25 U/mL Turbo DNase) and used for ribosome protected footprint isolation. The RNA for RNA-seq was isolated from the lysates with Zymo QuickRNA Miniprep kit. Ribo-seq: as described in McGlincy and Ingolia, 2017. RNA-seq: SMARTer Pico Input Mammalian RNA-seq kit v2 (Takara)