eDNA-Coastal-Lagoons
he modified universal COI primers mlCOIintF/jgHCO2198 (Leray et al., 2013) were used for PCR amplification of a fragment of ?313 bp within the mitochondrial gene coding for the COI. The primers were modified to include Illumina sequencing adapters and sample-specific dual indices (i5 and i7) following the Illumina (2013) protocol in which the conditions for the amplicon PCR were changed for the ones described by Leray et al. (2013). The PCR reactions were undertaken by Macrogen, following the protocol Illumina (2013). After constructing the library, it was charged into the platform Illumina MiSeq v3 reagents that generates paired-end sequences (2  301).