GSM5939264: cDC vehicle ATAC; Mus musculus; ATAC-seq
Spleens were removed and dendritic cells sorted. Cells were lysed in cold ATAC-seq cell lysis buffer and exposed to Tn5 transposase (Nextera) for 30min at 37C Transposed chromatin was amplified using customized Nextera PCR Primer 1 and Primer 2 (barcode) and HotStart KAPA ReadyMix (Roche)