GSM5811784: scNT-seq of HSPC day 4 timepoint; Homo sapiens; RNA-Seq
For the human hematopoiesis dataset, we cultured primary human CD34+ hematopoietic stem and progenitor cells obtained from the Fred Hutchinson Cancer Research Center. Cells were thawed and cultured in StemSpan SFEM II human hematopoietic stem cell expansion media (StemCell Technologies, Cat#02690) supplemented with StemSpan CC100 (StemCell Technologies, Cat#02690) and 50 ng/ml TPO (PeproTech, Cat#300-18-100UG). Cells were allowed to differentiate over the course of 1 week.