GSM5860196: jub1_ACC_Exp2; Arabidopsis thaliana; RNA-Seq
Total RNA was isolated by a "single-step" Trizol based method. DNAse treatment was carried out on 4 µg of RNA and the removal of DNA was validated by qPCR using intron-specific primers. RNA integrity was validated by an Agilent 2100 Bioanalyzer. Library preparation and RNA sequencing (RNA-Seq) was carried out at BGI Genomics and performed according to the BGISEQ-500 workflow.