GSM5917814: Mut2_cl2_patient_wk4; Homo sapiens; RNA-Seq
RNA was isolated according to the manufacturer's instructions and subsequently digested with TURBO DNase to remove trace quantities of DNA (ambion, Life Technologies). Before RNA-seq library preparation, assessment of RNA integrity numbers was performed using Agilent TapeStation. Libraries were pooled and sequenced using an Illumina HiSeq 2500 with 75-bp paired-read chemistry