BCR-seq of human: adult male PBMC
RNA was prepared from frozen PBMCs using the RNAEasy Mini kit (Qiagen) per the manufacturers instructions. BCR and TCR RNA libraries were prepared using reagents from New England Biolabs as part of the NEBNext Immune Sequencing Kit. These steps were performed as described previously with the inclusion of an additional set of TCRB-specific primers on the 3 end. Libraries were sequenced by 325 cycles for read 1 and 275 cycles for read 2 base-pair paired-end sequencing with a 20% PhiX spike on the Illumina MiSeq platform according to manufacturers recommendations.