RNA-seq of Mus musculus: 16.5 days post-coitus ovary
Total RNA was extracted from mouse ovaries according to the above protocol at 16.5 days post-coitus (dpc). Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, we established a transcriptome sequencing library and assessed library quality on an Agilent Bioanalyzer 2100 system. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using a TruSeq PE Cluster kit v3-cBot-HS (Illumina) according to the manufacturer's instructions. After cluster generation, the library preparations were sequenced on the Illumina NovaSeq platform, and 154 bp paired-end reads were generated.