GSM5011582: iKras_p53_Tumor_A; Mus musculus; RNA-Seq
Samples were mechanically and enzymatically (Collagenase P; 1mg/mL in DMEM) digested under constant agitation. Single cell suspension was obtained by filtering through a 40 µm mesh. Dead cells were removed using MACS®Dead Cell Removal Kit (Miltenyi Biotec Inc.). Single-cell cDNA libraries were prepared and sequenced using the 10x Genomics Platform at the University of Michigan Advanced Genomics Core.