GSM5962346: Sk-BR-3_FR_3; Homo sapiens; RNA-Seq
The RNAs of cells were released by using 0.2% Triton for 10 min while remaining DNAs within nucleus, and then the DNAs were released by using the alkaline lysis buffer (Vazyme Biotech, N603). DNA libraries were constructed according to MDA (Multiple Displacement Amplification) method by using random primers and φ29 DNA polymerase to amplify DNAs, and RNA libraries were constructed according to Smart-seq2.