Pol2 ChIP-seq of cdc17-ts-FRB POL2-3xFLAG, 40 minutes after G1, 37C + rapamycin
Mouse anti-FLAG (M2, F3165 Sigma) antibody was used for ChIP. Sequencing libraries were prepared as previously described (Belsky et al. 2015) with the following modifications: NEBNext multiplex oligos for Illumina Kit (New England Biolabs).