ITS sequencing of microbiome, fresh field soil
All PCR amplifications were performed according to Earth Microbiome Project standard protocols, including the designated primer pairs and thermocylcing conditions  (Thompson et al., 2017). Libraries were built from the resulting amplicons and sequenced at the University of Minnesota Genomics Center using the MiSeq platform (V3 chemistry; Illumina, San Diego, CA, USA) and a dual-indexed approach (Gohl et al., 2016).