AB5 Targeted Exome sequencing
Genomic DNA was isolated using DNeasy kits (Qiagen). Targeted deep-sequencing was done using custom-designed Nimblegen SeqCap EZ Choice Library baits to capture all coding exons of 253 known cancer genes. Nimblegen target capture and DNAseq library construction were done per the manufacturer's protocol (SeqCap EZ HyperCap Workflow).