GSM4962701: Fg349-MS2-WT-RNA_1; Fusarium graminearum; RNA-Seq
Mycelia were harvested by filtering, rinsed twice with sterile deionized water, and flash frozen in liquid nitrogen. One milliliter of TRIzolTM Reagent (Thermo Fischer Scientific) was added to 200 mg of mycelium before grinding for 1.5 min at 30 Hz using a TissueLyzer (Qiagen). Total RNA was then extracted using a previously published protocol (Hallen et al., 2007). The NEBNext Ultra Directional RNA Library Prep Kit for Illumina (cat. # E7420L New England BioLabs) was used to prepare all RNA-seq libraries, following the manufacturer's instructions. Sequencing was performed by the GenomEast platform, a member of the 'France Génomique' consortium (ANR-10-INBS-0009). Samples were run in 9-plex on an Illumina HiSeq 4000 in paired mode, 2x50 bp.