Genome sequencing of APA J-5(13-2)
The genome of APA J-5(13-2) was sequenced using an Illumina HiSeq X system at the Majorbio Bio-pharm Technology Co.,Ltd (Shanghai, China). Genomic DNA was sheared randomly to construct three read libraries with lengths of 400bp by a Bioruptor ultrasonicator (Covaris) and physico-chemical methods. The paired-end fragment libraries were sequenced according to the Illumina HiSeq X systems protocol. Raw reads of low quality from paired-end sequencing (those with consecutive bases covered by fewer than five reads) were discarded.