GSM5011324: DL01; Bos frontalis; RNA-Seq
All the samples were frozen in liquid nitrogen immediately and stored at -80℃ until further processing.Total RNA of each muscle sample was extracted from approximately 50 mg of frozen tissue using TRIzol Reagent (Invitrogen Corporation, Carlsbad, CA, USA) according to manufacturer recommendations. The quality of the RNA samples was evaluated in an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). After the quality control, the library preparations were sequenced on an Illumina Hiseq2000 platform (Illumina, San Diego, CA, USA) and paired-end reads were generated. RNA libraries were prepared for sequencing using standard Illumina protocols