GSM6126065: BKO [snRNA-seq]; Mus; RNA-Seq
Nuclei were isolated from frozen iBAT samples for 10x snRNA-seq. Each sample was pooled from 4 mice for each group. The nuclei suspension was loaded into Chromium microfluidic chips with 30 v3.1 chemistry and barcoded with a 10× Chromium Controller (10X Genomics). RNA from the barcoded cells was subsequently reverse-transcribed and sequencing libraries constructed with reagents from a Chromium Single Cell 30 v3.1 reagent kit (10X Genomics) according to the manufacturer's instructions. Sequencing was performed with IlluminaNovaseq 6000 according to the manufacturer's instructions (Illumina). 10x Genomics Single Nucleus RNA-seq