GSM5608347: Giardia lamblia WB: 5pac_5n control; Giardia intestinalis; RNA-Seq
Total RNA was extracted by Trizol® Reagent (Invitrogen, USA) according to the instruction manual. RNA purified was quantified at OD260nm by using a ND-1000 spectrophotometer (Nanodrop Technology, USA) and qualitated by using a Bioanalyzer 2100 (Agilent Technology, USA) with RNA 6000 labchip kit (Agilent Technologies, USA). All procedures were carried out according to the manufacture's protocol from Illumina. Library construction of all samples were used by TruSeq RNA Sample Prep Kits v2 for   50SE    bp (Single-End or Paired-End) sequencing on Solexa platform. The sequence was directly determined using sequencing-by-synthesis technology via the TruSeq SBS Kit. Raw sequences were obtained from the Illumina GA Pipeline software CASAVA v1.8 and expected to generate   10M    (million reads or Gb) per sample.