GSM5560964: Bisulfite-seq_NSD1; Mus musculus; Bisulfite-Seq
DNA extracted using phenol-chloroform protocol after RNAse treatment. DNA was bisulfite converted with the EZ DNA methylation kit (Zymo). DNA were spiked with 1% unmethylated lambda phage DNA. Libraries were generated by post bisulfite adaptor tagging method with 8 cycles of library amplification.