Small RNA seq of Chaoborus americanus SNOA1 eggs
Total and small RNAs were purified using miRNeasy columns (Qiagen). Indexed small RNA libraries were prepared using the NEBNext Multiplex Small RNA Library Prep Set (New England Biolabs). cDNA libraries were size selected from a 5% polyacrylamide gel by excising fragments corresponding to insert sizes of 19 to 39 bp for siRNAs and piRNAs.