NextSeq 500 paired end sequencing; RNAseq of human B-ALL cell lines following RUNX1 or CBFb knock-down
RNAseq of human B-ALL cell lines following RUNX1 or CBFb knock-down
Viable, GFP+ cells sorted by flow cytometry, washed in PBS, lysed in Trizol Standard trizol protocol Illumina Truseq