GSM5563278: G401 ATAC DMSO; Homo sapiens; ATAC-seq
Cells were collected and lysed to obtain purified nucleus; The Tn5 enzyme transposable reaction was carried out and the open regions of chromatin were fragmented; the fragmented DNA was purified; the purified DNA was amplified. The amplified DNA was sized to remove non-target fragments. Librarypreparation was performed using TruePrepTM DNA Library Prep Kit V2 for Illumina kit. 1.2x agencourt Afure XP beaas(Beckman Coulter)were used to purify libraries for sequencing .150 paired-end sequencing was performed with Navoseq6000.