GSM6285004: Endo_Failed_7; Homo sapiens; RNA-Seq
For RNA-seq analysis , total RNA were collected using NucleoSpin RNA (MACHEREY-NAGEL) for mice and the RNeasy Plus Mini Kit (Qiagen) for humans, with genomic DNA elimination. For ChIP-seq analysis, to extract chromatin, isolated day 6 endometrial cells were suspended in 20 µl of Nuclei EZ Lysis Buffer (SIGMA), and extensively digested chromatin with 20 IU/µl Micrococcal Nuclease (MNase, NEB) at 37℃ for 5 min. RNA-seq library preparation was carried out using TruSeq Stranded mRNA Library Prep kit (Illumina) following manufacturer's instructions. ChIP-seq library preparation was carried out by NEBNext ChIP-seq for Illumina (NEB) following manufacturer's instructions.