GSM5560985: CGR8 cells, FBS_Ctrl_3; Mus musculus; RNA-Seq
Cells were harvested in 1ml of TRIzol reagent (Thermo Fisher Scientific ®) and total RNA was extracted according to manufacturer's instructions. RNA was quantified and integrity was mesured by optic density using a 2100 BioAnalyzer TruSeq Stranded Total RNA H/M/R- Gold, ILLUMINA®. rRNAs were depleted through magnetic beads (Agencourt® RNAClean XP, BECKMAN COULTER).