Ago PAR-CLIP of BCKN1
Ago (Argonaute) PAR-CLIP experiments were performed on Epstein-Barr virus (EBV) infected diffuse large B cell lymphoma cell lines (DLBCLs) as previously described (Hafner, 2010; Skalsky, 2012) with minor modifications. Cells were cultured in 100 uM 4-thiouridine and cross-linked at UV 365 nm. Ago immunoprecipitations were carried out with either Abcam ab57113 antibodies (Ago) or Diagenode 2A8 monoclonal mouse antibodies (Ago2A8). cDNA libraries were prepared using the Illumina TruSeq small RNA library prep kit.