GSM5964203: C2C12 on 0.5Kpa-soft at day 0 [D0S]; Mus musculus; RNA-Seq
Cells were harvested using 0.05% Trypsin/EDTA. RNA was extracted using RNeasy micro kit with a start amoung of RNA of 100ng. RNA libraries were prepared using NEBNext Poly(A) mRNA Magnetic Isolation Module(E7490S), with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina(E7760) and Multiplex Oligos for illumina(96 Unique Dual Index Primer Pairs) (E6440)