GSM5116042: ESF_hypoxia_B_input; Homo sapiens; ChIP-Seq
Cells were harvested, resuspended and chemically cross-linked in suspension by the addition of 1% fresh formaldehyde with 10 minutes incubation at room temperature on a rotator. Cross-linking was quenched and cells were harvested by centrifugation and washed twice in cold PBS. Nuclei were extracted and sonication was performed using a Misonix S4000 sonicator.  Fragment size between 200bp and 600bp was obtained and confirmed by gel electrophoresis and Bionalyzer. For each H3K4Me3 ChIP sample also an input chromatin sample was sequenced. DNA library preparation and high-throughput sequencing were performed on an Illumina Genome Analyzer II following the protocol recommended by Illumina with a 75bp readleght at the Yale Center for Genome Analysis.