GSM4742468: AR_45: Control; Homo sapiens; miRNA-Seq
Total RNA isolation was carried out from all serum samples (450 µl) by miRNeasy Serum/Plasma Kit (Qiagen GmbH, Hilden, Germany). The isolated RNA was held frozen at −80°C until further use. RNA integrity and absence of DNA was confirmed by Bioanalyzer RNA Nano chips (Agilent Technologies, St. Louis, MO) and Qubit DNA High sensitivity kit, respectively. Sequencing libraries were prepared using the Illumina TruSeq Small RNA Library Preparation Kit. Next generation sequencing was performed on 2 lanes of an Illumina HiSeq2500 (Illumina, San Diego, CA) multiplexing all samples (single end read, 50 bp).