GSM5525109: methylome_Obir_WT_rep1; Ooceraea biroi; Bisulfite-Seq
MethylC-seq libraries were constructed using the MethylC-seq protocol (Urich et al., 2015). Briefly, genomic DNA was sonicated to around 200 bp using a Covaris S-series focused ultrasonicator, and end-repaired with an End-It DNA end-repair kit (Epicentre). The end-repaired DNA was subjected to A-tailing using Klenow 3′–5′ exo− (NEB) and ligated to methylated adapters using T4 DNA ligase (NEB). The ligated DNA was treated with sodium bisulfite reagent using the EZ DNA methylation-Gold kit and amplified using KAPA HiFi uracil + Readymix Polymerase. Sequencing was performed on an Illumina NextSeq500 instrument.