GSM5115982: ZSTK474; Mus musculus; RNA-Seq
Single-cell suspensions of foetal thymocytes were obtained by gently homogenizing thymic lobes followed by straining through nylon strainers. Cells were stained for cell surface markers, followed by sorting of total γδ T cells from each sample, control and ZSTK474-treated. Single cell RNA sequencing libraries were generated using the Chromium™ Single Cell 5' Library and Gel Bead Kit (10X Genomics) according to the manufacturer's instructions. Uniquely indexed libraries were pooled for paired-end sequencing (Illumina NextSeq).