GSM5983486: N3uM1; Homo sapiens; RNA-Seq
Bacterial DNA extraction from faecal samples and fermentation samples using QIAamp® PowerFecal® DNA kit The Illumina sequencing platform was used to perform high-throughput sequencing of 16S rRNA (V3-V4 high-variance region) in 10 groups of microbial communities in faecal samples. The data obtained were screened, optimized and spliced, and then quality-controlled to obtain high-quality sequences, and the high-quality sequences were clustered at 97% similarity and chimerically filtered to obtain OTUs for species classification