DMS of NEB 5-alpha F'Iq: Single-codon substitutions in NDM-1, sequencing run 1, generation 0
A library of single-codon substitutions in NDM-1 was constructed using inverse PCR. The library was transformed into NEB 5-alpha F'Iq cells. We plasmid prepped uninduced, exponentially-growing cultures of the library mixed with wildtype NDM-1 and determined the allele frequencies at the start of the growth experiment as our generation 0, replica 1 sample. Data for codons 1-271 was used from these files. This was used as Replica 1 for regions 1 (codons 1-90), 2 (codons 91-180), and 3 (codons 181-271).