GSM6067599: R11390 CD Uninfected T0; Homo sapiens; RNA-Seq
Total RNA was extracted from MDMs following the double extraction protocol:  RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction (TRIzol, Thermo Fisher Scientific, Waltham, MA, USA) followed by a Qiagen RNeasy Micro clean-up procedure (Qiagen, Hilden, Germany). cDNA libraries were prepared using 2 ng of total RNA using the SMARTSeq v2 protocol with the following modifications: 1. Addition of 20 µM TSO; 2. Use of 200 pg cDNA with 1/5 reaction of Illumina Nextera XT kit (Illumina, San Diego, CA, USA).