GSM4962548: pancreatic ductal adenocarcinoma cells, sgPaf1-2 Paf1KD RNA-seq; Mus musculus; RNA-Seq
Before RNA extraction, 0.2% Drosophila S2 cells were mixed with PDAC cells as spike-in. RNA was extracted with TRIzol (Invitrogen, 15596018) according to the manual 500ng RNA of each sample was used to prepare libraries. Total RNA-seq libraries were prepared using the VAHTS Total RNA-seq (H/M/R) Library Prep Kit for Illumina (Vazyme, NR603-01).