GSM6172687: shPRM1-2; Mus musculus; RNA-Seq
Total RNAs were extracted using QIAsheredder (Qiagen, #79656) and Qiagen RNeasy mini kit (Qiagen. #74106). Library preparation (Roche, Kapa mRNAseq Hyper prep) and sequencing analysis (Illumina NextSeq 500 Paired-end 2 x 150 bp) were performed at the Molecular Biology Core facility in Dana-Farber Cancer Institute.