Transcriptome  analysis of Mesorhizobium huakuii 7653R and its derive strain 7654R-mreB in TY medium and nodule on Astragalus sinicus.
MRNA was enriched by stripping rRNA through Ribo-Zero kit, and mRNA was broken into short fragments by fragmentation buffer. MRNA was used as template, a cDNA was synthesized with random hexamers, and then the buffer DNTPs (DTTP in DnTP was replaced by Dutp) and DNA Polymerase I and RNase H were added to synthesize two cDNA strands, and then AmPure XP was used After purification of double strand cDNA in beads, USER enzyme was used to degrade the second purified double strand cDNA containing U. The second strand of double strand cDNA was purified by terminal repair and A tail and connected with sequencing adapters. Then AMPure XP Beads were used for fragment size selection and finally PCR amplification