GSM5738251: D51; Danio rerio; RNA-Seq
After the zebrafish kidney was obtained by dissection, it was lysed in trizo, then extracted with PCI / CI, precipitated with isopropanol and eluted with 75% alcohol to obtain total RNA After the total RNA sample is tested to be qualified, magnetic beads with oligo (DT) are selected for enrichment and purification. Fragment buffer is added to the purified mRNA to make its fragment into a short fragment. Then the second strand of cDNA is synthesized with the fragment mRNA as a template and purified by qiaquick PCR kit. The purified double stranded cDNA was then treated by terminal repair, base A and sequencing. Then the target fragment was recovered by agarose gel electrophoresis and PCR was amplified to complete the whole library preparation.