Timestamp 3T3 Cells:Dox and Vivid
PCRed using P5 and a barcoded version of SGR-176 (see Table S2) the Q5 Hot Start High Fidelity 2x Master Mix (NEB, M0492L) with the following settings: 30s of 98C denaturation; then 25-30 cycles of 10s denaturation at 98C, 20s annealing at 70C, and then 25s extension at 72C. PCR reactions were then pooled and run on a gel, and a 400bp band was extracted using the NucleoSpin PCR Cleanup Kit (Macherey-Nagel, 740609.250).