GSM6125602: K562 10ng 2 min heat; Homo sapiens; Bisulfite-Seq
gDNA was extracted using Qiagen Dneasy Blood and Tissue kit. gDNA was fragmented using the LE220 Focused ultrasonicator (Covaris) and size selected for 100-200 bp fragments using BluePippin 2% agarose cassette (Sage Sciences). End repair and A-tailing using Kapa hyper prep kit and methylated Truseq nano adapters. Bisulfite conversion was done using Zymo EZ DNA Methylation-Gold kit