GSM5827491: s15; Homo sapiens; miRNA-Seq
Circulating miRNAs were isolated from the serum using the NucleoSpin miRNA Plasma, Mini kit for circulating miRNA (Macherey-Nagel) according to the manufacturer`s protocol. Libraries for sequencing were prepared from 10.5 µL of isolated miRNAs using a NEXTflex Small RNA-Seq Kit v3 (Bioo Scientific) according to the manufacturer`s protocol. small RNA libraries were prepared for sequencing using standard Illumina protocols