GSM5827444: KO2-ASC (Bulk KO vs. WT SMC); Mus musculus; RNA-Seq
Ascending aorta/aortic arch or descending thoracic aorta segments were enzymatically digested under cold conditions, single (based on SSC-W vs SSC-H and FSC-W vs FSC-H gates), viable (based on exclusion of viability dye), GFP+ SMCs were sorted into NEB lysis buffer, and between 9,000-20,000 cells from individual aortic segments were analyzed for each sample NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB #E6420)