GSM5119127: HD15; Homo sapiens; RNA-Seq
PBMC were thawed, washed and cells were stained with fluorophore-conjugated +- DNA-conjugated antibodies (for cohort 2) for cell sorting by flow cytometry and sample pooling for single cell RNAseq library construction (10x Genomics, Single Cell 3′ Reagent Kit (V2 or V3 chemistry, cohort 1 and cohort2, respectively) RNA libraries were prepared for sequencing using standard 10X Genomics Single Cell 3′ Reagent Kit (V2 or V3 chemistry)