GSM5009609: 5µM_3-7_compound_THP1_r3; Homo sapiens; RNA-Seq
Cells were counted by Trypan Blue Exclusion, washed with PBS and cell pellets frozen at -80 in Qiagen RLT buffer. Triplicates were processed together by Qiagen microRNA extraction kit according to manufacturers instructions - RNA quantities were assessed by Nanodrop and quality assessed using the Agilent RNA integrity 6000 nano kit RNA libraries were prepared for sequencing using Standard TruSeq Stranded mRNA Illumina protocols followed by a quality check using Agilents High Sensitivity DNA kit