QsucCho001
We used the Unioverse probe set (Pfeiffer et al., 2019) to capture phylogenetically informative nuclear protein-coding loci. Sequencing libraries, capture, and Illumina sequencing were carried out at RAPiD Genomics (Gainesville, FL, USA). Libraries were constructed by shearing DNA to an average length of 400 bp followed by an end-repair reaction and ligation of anadenine residue to the 3-end of each blunt-end fragment. Barcoded adapters were ligated to the library followed by PCR amplification of the libraries. Libraries were pooled into groups of up to 16 samples and the SureSelectxt Target Enrichment System for Illumina Paired-End Multiplexed Sequencing Library protocol was followed for solution-based target enrichment. The probes were synthesized as Custom  SureSelect probes from AgilentTechnologies (Santa Clara, CA, USA). An Illumina HiSeq 3000 (San Diego, CA, USA) was used to generate 150-bp, paired-end reads.