GSM5563656: EXO-ADSC1; Homo sapiens; miRNA-Seq
Total RNA was extracted using mirVana miRNA Isolation Kit (Ambion) according to the manufacturer's protocol. Quantitation of total RNA was carried out using the Nanodrop 2000 (Thermo Fisher Scientific Inc., USA). RNA integrity was assessed by Agilent 2100 Bioanalyzer (Agilent Technology, USA). 1 μg total RNA of each sample was used for the small RNA library construction. Libraries were prepared according to Illumina's instructions accompanying TruSeq Small RNA Sample Prep Kits (Cat. No. RS-200-0012, Illumina, USA.). Briefly, total RNA were ligated to adapters at each end. Then the adapter-ligated RNA were reverse transcribed to cDNA and performed PCR amplification. The PCR products ranging from 140–160 bp were isolated and purified as small RNA libraries. Library quality was assessed on the Agilent Bioanalyzer 2100 system using DNA High Sensitivity Chips. The libraries were finally sequenced using the Illumina NovaSeq6000 platform. 150 bp paired-end reads were generated. The small RNA sequencing and analysis were conducted by OE Biotech Co., Ltd. (Shanghai, China).