GSM4742961: DMSO_ATAC; Homo sapiens; ATAC-seq
5×10^4 cells were collected and lysed using lysis buffer (10 mM Tris-HCl at pH 7.4, 10 mM NaCl, 3 mM MgCl2 and 0.1% NP-40) to collect cell nuclei. Then, nuclei were resuspended in the mixture containing 10 μl 5× TTBL, 5 μl TTE Mix V50 and 35 μl ddH2O. The mixture were incubated at 37°C for 30 min. Then, the fragments were purified by the standard processing of TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme) and VAHTS DNA Clean Beads (Vazyme N411). The standard processing was performed using TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme) and TruePrep Index Kit V2 for Illumina ® (Vazyme TD202). The library concentration was measured using Qubit dsDNA BR Assay Kit (Invitrogen) by Qubit (Invitrogen).