GSM5115831: DA and FL, E10.5; Mus musculus; RNA-Seq
Tissues were dissociated using 1 mg/ml collagenase/dispase and then 0.25% trypsin-EDTA. Single cells were lysed in droplets using the 10X Chromium. Reverse transcription was performed in droplet. Library construction was performed by using 10X Chromium Single Cell 3' v3.1 kit.