Illumina HiSeq 2500 sequencing; RNA-seq comparing WT to Dax1, Nr5a2 and Dax1/Nr5a2 knock-out clones in 2iLIF and SL differentiation conditions
RNA-seq comparing WT to Dax1, Nr5a2 and Dax1/Nr5a2 knock-out clones in 2iLIF and SL differentiation conditions
total RNA collected Cells grown in 2iLIF on gelatin coated plates and transferred to SerumLIF 1nM Retinoic Acid on gelatin for 48h G6C18 mouse embryonic stem cells, transfected with gRNAs AGAGCCGACCGCATGCGAGG and AAAAGTTCCAGTATCAGGTG, mutant clone RNeasy Mini Kit with on-column DNase digest (Qiagen) TruSeq Illumina mRNA-seq (stranded - high input)