GSM5859136: D5 control rep2; Mus musculus; ATAC-seq
ATAC-seq was performed as previously described (Buenrostro et al. Nat Methods 2013). 150,000 cells were collected from GLI1 OE and control samples. Cell samples were then lysed, as described (Buenrostro et al. Nat Methods 2013), and transposition was carried out at 37C for 30 minutes with Tn5 enzyme. Libraries were generated from transposed DNA using Illumina's Nextera DNA Library Prep kit (Illumina 15028212).