GSM5827688: human fetal ovary, 11w4d; Homo sapiens; RNA-Seq
The human fetal ovary was collected after informed consent from participants undergoing elective abortion or gynecological procedures.  All experimental procedures were approved by the Institutional Review Board at Kyoto University (G1047 and G1192).  The fetal ovary were carefully dissected out in chilled PBS and minced with scissors in 0.05% trypsin/EDTA/PBS solution (GIBCO, 15400-054), then incubated for 15 min at 37℃ with gentle pipetting every 5 min.  Dissociation medium [DMEM (GIBCO, 10313021) containing 10% FBS, 2mM L-glutamine (GIBCO, 25030081), 1X penicillin/streptomycin (GIBCO, 15070063), and 100μg/mL DNaseI (MERCK, DN25)] was added for further dissociation.  Dissociated cells  were stained with DRAQ7 to distinguish dead cells.  All DRAQ7(-) live cells were collected by using FACS (BD Biosciences, FACS Aria III). The cDNA library for NGS was conducted with Chromium Next GEM Single Cell 3' Kit v3.1 (Single Index) according to the manufacturer's instructions. scRNA-seq