GSM5563734: PBS1; Homo sapiens; ncRNA-Seq
After Hp26695-infected AGS cells were infected at a multiplicity of infection (MOI)  = 100:1 for 12 h, RNA was harvested using Trizol reagent. The control group used PBS for the same amount of time.Pretreat 5 μg total RNA, and use CircRNA Enrichment Kit (Cloud-seq, USA) to enrich circular RNA. Use TruSeq Stranded Total RNA Library Prep Kit (Illumina, San Diego, CA, USA) to preprocess RNA and construct a sequencing library; Use BioAnalyzer 2100 instrument (Agilent Technologies, USA) for library quality control and quantification. According to the Illumina sequencing instructions, the library was denatured into single-stranded DNA molecules, captured on an Illumina flowcell, amplified into clusters in situ, and 150 cycle sequencing was performed on an Illumina HiSeq sequencer.