GSM5608522: HSV-1 wt, strain 17, +PAA, replicate 1; Homo sapiens; ATAC-seq
Infected cells were harvested by scraping at the indicated time points, washed, lysed with mild lysis buffer according to the published ATAC-seq protocol, incubated with Tn5 transposase (tagmentase) for 30 minutes, lysed in Qiagen's PB buffer, DNA purified according to the MinElute protocol. 12 PCR cycles according to the Illumina Nextera protocol using barcoded Nextera Primers. 35bp paired-end, NextSeq 500 (Illumina)