sRNA-seq of SH-SY5Y: PUM1 + PUM2 KD
3ug of each RNA sample was used for small RNA library preparation. Libraries were generated using NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs) according to manufacturers protocols. Small RNAs with 5' and 3' adaptors are made into cDNA and amplified via RT-PCR then separated by PAGE for size selection