GSM5939409: L136Rb_1_liv_stat_RNAPChIP [R168b_1_liv_stat]; Escherichia coli; ChIP-Seq
Cell pellets were lysed and sonicated. Input samples were set aside and the remainder of each lysate was split in half where the Lrp-DNA complexes and RNAP-DNA complexes were isolated with their respective antibodies;  Neoclone W0023 (the RNA polymerase antibody),  lot # 2019G15-002, Lrp antibody is NeoClone custom antibody clone# 1G4D9A10, Lot# 2016E25-001. ChIP-seq libraries were prepared for sequencing with the NEBNext UltraII DNA kit using an OpenTrons2 robot