RNAseq  of bronchial epithelium; Severe asthma with no GORD GA3 005
Total RNA was extracted from epithelial brushings using miRNeasy Mini Kit and RNase-Free DNase Set (Quiagen, Manchester, UK). cDNA libraries were prepared using NEBNext Ultra (non-stranded) mRNA library prep kit with polyA pulldown for mRNA enrichment. Paired-end 150bp sequencing to a depth of 20M reads (epithelial brushings) or 50M reads (differentiated cells) was performed on an Illumina HiSeq2500 by Novogene Inc