GSM5963971: F47-PLow-S4_B; Homo sapiens; RNA-Seq
For Experiment 1 cells were collected in TRIzol, and total RNA was purified using a PureLink RNA Mini Kit (Life Technologies, 12183025) according to the manual. DNA was removed using a DNA-free DNA Removal Kit (Life Technologies, AM1906) according to the manual. For Experiment 2 cells were collected and total RNA was purified using a QIAGEN RNeasy Mini Kit (QIAGEN, 74104) with no DNase treatment. Sequencing libraries were constructed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7760). The sequencing library construction process included mRNA purification with polyA selection beads, fragmentation to inserts of ~200 bp, strand specific cDNA synthesis, end repair, 3' end adenylation, adapter ligation, and PCR amplification. Samples were run across 3 flow cells (Experiment 1: A, B, and C; Experiment 2: 708, 709, and 710).