NGS of SARS-CoV-2: mixed infection
RNA is extracted from 400 l of patient anterior nares sample using the MagMAX Viral/Pathogen kit (ThermoScientific). All samples are subjected to total RNA library preparation using the Rapid RNA Library Kit Instructions (Swift Biosciences). SARS-CoV-2 genome capture is accomplished using hybridization kit xGen COVID-19 Capture Panel (Integrated DNA Technologies).