GSM5827452: WT-1 Single cells aorta; Mus musculus; RNA-Seq
Thoracic aortas were enzymatically digested under cold conditions, single (based on SSC-W vs SSC-H and FSC-W vs FSC-H gating), viable (based on exclusion of viability dye) cells were sorted into buffer with 0.04% BSA, and 4,300 to 6,278 cells from individual aortas were analyzed for each sample Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1