GSM5939997: WT, Ctf3-myc, IP, replicate2; Saccharomyces cerevisiae; ChIP-Seq
Exponential yeast cells were crosslinked with 1% formaldehyde and lysed in a Mini-beadbeater. The chromatin was sonicated, quantified and incubated with anti-myc antibody overnight followed by 2 hr incubation with Protein G-Dynabeads. The non-specific binding was removed by washing and DNA was eluted by reverse crosslinking and Proteinase K digestion, followed by processing for Illumina DNA sequencing libraries. Libraries were sequenced on an Illumina NextSeq 500 by the UVA Genome Analysis and Technology Core. Libraries were made according to Illumina's TrueSeq protocol