Assembled genome of Arthroderma multifidum
First scrape the Fungal from medium, extract nucleic acids with a commercial extraction kit (Digena) , add lysate and transfer it to a centrifuge tube with glass bead, grind the tube at 2000rpm for 3min, and take the supernatant and mix with combined solution, then added to the spin column, and the waste liquid is removed by centrifugation. After washing with washing solution, the nucleic acid is eluted. The nucleic acid should meet the quality (greater than 1 microgram) and length requirment(agarose gel electrophoresis, greater than 5000bp), then the library can be constructed.The nucleic acid is purified by magnetic beads after nick repair and end repair, then the sequencing adapter and motor protein are ligated. After purification again, the concentration is qualified with Qubit, then prepare the chip. After selecting the appropriate parameters and modes, the solution was sequenced on the machine.The basecaller module in guppy software is used to convert the changed current signal value data of sequencing into base sequences to generate fastq data files.