GSM5827050: amhc:EGFP+ nr2f1a mutant; Danio rerio; RNA-Seq
For amhc:EGFP+ and amhc:EGFP+/myl7:DsRED-NLS cells, they were flow sorted following digestion of whole embryos at 48 hours post-fertlization. For RNA, the RNA was harvested using TRIZOL and a PureLink RNA Micro Kit (Invitrogen). The libary was then amplified using a Single Cell Purification Kit (Norgen Biotek). For ATAC-seq, the harvested cells were processed for ATAC-seq with the Tn protein and libraries generated. RNA and ATAC libraries were prepared for sequencing using standard protocols