MinION sequencing of Staphylococcus aureus strain ATR-20003 isolated from contaminated human platelets
Single colony grown O/N at 35 degrees in 5mL TSB-YE and pellet resuspended in Zymo DNA/RNA Shield. DNA extraction using Zymo Quick-HMW kit with Lysozyme and RNAseA treatment. DNA was processed (SPRI selection >1500). Rapid barcoding sequencing kit (SQK-RBK-004) and 1D MinION sequencing using FLO-MIN106 R9.4 flowcell