GSM5859098: TGA2.1-NahG_control_PVY_3 [OE362]; Solanum tuberosum; RNA-Seq
Early visible lesions and their immediate surrounding area were sampled from PVY-inoculated leaves four days post inoculation. Sections of comparable size were sampled from mock-inoculated leaves as controls. Three plants per genotype per treatment were analysed, pooling together all lesions or mock-sections from one leaf per plant. Tissue sections were stored in RNA-later RNA Stabilization Solution (ThermoFisher Scientific) and then homogenized using Tissue Lyser (Qiagen). Total RNA was isolated with TRIzol (Invitrogen) and Direct-zol RNA MicroPrep Kit, DNAse treated and purified with RNA Clean & Concentrator kit (both Zymo Research) according to the manufacturer's instructions. Strand-specific library preparation was performed by Novogene (HongKong) using standard Illumina protocol.