V4Amplicon of microbiota from rattus norvegicus:milk inocula from lactating dams
Inocula prepared from milk and faeces collected from obese prone or obese resistant dams were transferred to F344 pups from their 1rst to 15th days of life, while some others pups being transferred with diluant in order to constituted sham-controls. The composition of both inocula and recipients intestinal microbiota (at different developmental stages) has been characterized by sequencing of the V4 region of 16S DNA at GeT-PlaGe platform (INRA-Toulouse, France) using Illumina MiSeq paired-end chemistry (2 x 250 bp reads), after DNA extraction and amplification with PCR using primers: 515F and 806R.