GSM6172711: WT [SAM24335533]; Homo sapiens; RNA-Seq
Total RNA was extracted using a RNeasy Mini Kit (Qiagen, cat 74104) and quantified in a NanoDrop 8000 (ThermoFisher). 0.1 μg of total RNA was used for library preparation with a TruSeq Stranded Total RNA Library Prep Kit (Illumina). The libraries were multiplexed and then sequenced on an Illumina HiSeq4000 (Illumina) to generate 30 million single end 50 bp reads.