GSM6068599: FAC sorted GFP cells, 15 s Gt(endocard:egfp), rep 1; Danio rerio; RNA-Seq
Embryos were collected, deyolked, and dissociated at the 15 somite stage. GFP positive cells were FAC sorted directly into trizol and total RNA extracted using a Direct-zol RNA MiniPrep kit (Zymo research) 2 ng of total RNA from each sample was used for the construction of sequencing libraries, libraries were prepared using the Smart-seq2 protocol as described by Picelli et al., 2014 (PMID:24385147)