GSM5859119: CD1 pSHF rep3; Mus musculus; ATAC-seq
ATAC-seq was performed as previously described (Buenrostro et al. Nat Methods 2013). The pSHF and atria (HT) were microdissected from CD1 embryos, pooled, and dissociated with TrypLE (ThermoFisher Scientific 12605-010). Cell samples were then lysed, as described (Buenrostro et al. Nat Methods 2013), and transposition was carried out at 37C for 30 minutes with Tn5 enzyme. Libraries were generated from transposed DNA using Illumina's Nextera DNA Library Prep kit (Illumina 15028212).