High-throughput sequencing of ITS2 aplicon fungal soil metagenome after cultivating on agar medium
Soils from multipolluted sites in Poland (SRR16146690, SRR16146688, SRR16146670) were homogenized and cultivated on Maltose Extract Agar medium with copper salts and BTEX mix added. After two weeks of incubation at 17C. Then, whole genomic DNA was extracted from the mycelium overgrowing plate. ITS2 rDNA fungal fragment was then amplified and sequenced on Illumina MiSeq in paired-end mode.