GSM4744896: ttpA-C240ins_0hr_sample_1B; Dictyostelium discoideum; RNA-Seq
RNA was purified using the Illustra RNAspin Kit (GE Healthcare Life Sciences) following the manufacturer's protocol. Total cellular RNA was quantitated with a Qubit fluorometer, and 250 ng of each sample was transcribed to generate cDNA libraries using the Illumina TruSeq RNA Kit (Illumina Inc, San Diego, CA) following the manufacturer's Low Sample (LS) protocol, with the following modifications:  2 minutes of centrifugation were used during the bead drying steps and 12 cycles were used to enrich DNA fragments.