Sandal Microbiome
Total metagenomic DNA was extracted from the sandalwood sapling sample following the modification of Yeates et al. (1998) and V3 - V4 regions were amplified by using primers. The first-round PCR products were used as templates for second-round amplicon enrichment of PCR. At the same time, indexed adapters were added to the ends of the 16S rRNA amplicons to generate indexed libraries ready for downstream NGS sequencing on Illumina Miseq.