GSM5827410: Scr_1; Homo sapiens; RNA-Seq
RNA was isolated from treated PAH PAAFs using the miRNeasy mini kit (Qiagen) combined with on-column DNase digestion (DNase-Free DNase Set, Qiagen) to avoid contamination by genomic DNA. 3µg total RNA was used as input for VAHTS Stranded mRNA-seq Library preparation following manufacturer's protocol (Vazyme).