16S rRNA gene sequencing of environmental aquatic and soil samples collected 17 years post-fire.
DNA was extracted from soil samples using the Zymobiomics Quick-DNA Fecal/Soil Microbe Kits (Zymo Research, CA, USA). 16S rRNA genes in extracted DNA were sequenced at Argonne National Laboratory using bacterial/archaeal primer set 515F/806R, which targets the V4 region and maximizes coverage of bacteria and archaea while also providing polymerase chain reaction products long enough for sequencing.