GSM5917704: T2; Homo sapiens; ncRNA-Seq
Total RNA was isolated using theRNeasy mini kit (cat#Qiagen, Germany), after which the RNAconcentration and quality were determined by the Qubit ® 2 .0 Fluorometer (Life Technologies,USA) and the Nanodrop One spectrophotometer (Thermo Fisher Scientific Inc., USA). Integrityoftotal RNA was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies Inc., USA),and samples with RNA integrity number (RIN) values above 7.0 were used for sequencing. Onemicrogram of RNA were used as input material for the RNA sample preparations. RNA libraries were prepared for sequencing using standard Illumina protocols