GSM5277232: shLuc; Homo sapiens; miRNA-Seq
RNA was extracted from frozen tumor samples using the miRNeasy Micro Kit (Qiagen) as recommended by the manufacturer. Libraries for miRNA sequencing were prepared from total RNA samples using NEBNext Multiplex Small RNA Library Prep Kit for Illumina. Each sample was sequenced on the Illumina NextSeq 550 to generate single-end 50 nucleotide reads.