GSM4904042: Sample1_UB_SingleCell; Homo sapiens; RNA-Seq
Single Cell RNA-Seq: Samples were dissociated with a 1:1 TrypLE/Accutase solution (~0.2 ml per organoid) at 37˚C for 15–25 min, with occasional agitation (flicking) until large clumps were no longer clearly visible. The cell suspension was then centrifuged (3 min, 300 g) and the dissociation solution removed. Cells  were resuspended in HBBS supplemented with 2% FBS and passed through a 40 μM FACS tube cell strainer. Following centrifugation at 300 g for 3 minutes, the supernatant was discarded and cells resuspended in 50 μl TeSR-E6 medium. Viability and cell number were assessed and samples were run across separate runs on a Chromium Chip Kit (10x Genomics) Single Cell RNA-seq: 10x libraries were constructed using 10x Chromium Next GEM Single Cell 3' Reagent kit v3.1. Sequenced using Illumina Novaseq