GSM6124467: A5398P; Homo sapiens; RNA-Seq
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with Zymo Direct-Zol RNA mini prep kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser. For directional RNA-seq with ribosomal RNA (rRNA) reduction, libraries were prepared using KAPA Stranded RNA-Seq Kit with RiboErase (HMR) (KapaBiosystems) and sequenced as single-end (SE) 75 base reads on an Illumina NextSeq 500 instrument at the Van Andel Research Institute Genomics Core. Total RNA sequencing with ribosomal RNA depleted