Whole Genome Sequencing of Pediococcus pentoseaus- Illumina data
50 ng of Qubit quantified DNA was enzymatically fragmented, end-repaired and A-tailed in a one-tube reaction using the FX Enzyme Mix provided in the QIASeq FX DNA kit. The end-repaired and adenylated fragments were subjected to adapter ligation, whereby index-incorporated Illumina adapter was ligated, to generate sequencing library. This library was subjected to 6 cycles of Indexing-PCR to enrich the adapter-tagged fragments. Finally, the amplified library was purified using JetSeq Magnetic Beads. Further, the Illumina-compatible sequencing library was quantified by Qubit fluorometer (Thermo Fisher Scientific, MA, USA) and the fragment size distribution was analyzed on Agilent TapeStation. The libraries were paired-end sequenced on Illumina HiSeq X Ten sequencer for 150 cycles following manufacturers instructions