GSM5860171: CnR_IgG_NPC_rep1; Homo sapiens; OTHER
50,000 cells were freshly harvested and bound to concanavalin A-coated magnetic beads after an 8min incubation at RT on a rotator. Cell membranes were permeabilized with digitonin and the different antibodies were incubated overnight at 4°C on a rotator. Beads were washed and incubated with pA-MN. Ca2+-induced digestion occurred on ice for 30min and stopped by chelation. DNA was finally isolated using an extraction method with phenol and chloroform as described in Skene et al., 2018 Libraries were prepared for sequencing using standard Illumina protocols