GSM6124436: WGBS Control, untreated wheat seedling 2; Triticum aestivum; Bisulfite-Seq
Plant materials were homogenized with Bertin Precellys Minilys. Genomic DNA was isolated with Macherey-Nagel NucleoSpin Plant II mini kit based on the manufacturer's user manual. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the firs strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on Illumina NovaSeq 6000 and MGI DNBSEQ-G400 platform. WGBS library was constructed with NEBNext® Enzymatic Methyl-seq Kit by the manufacturer's protocols Whole Genome Bisulfite Sequencing