GSM6067638: HCASMC-hTERT-FHL5_H3K4me3_rep1; Homo sapiens; OTHER
Genomic DNA  was released into the supernatant following antibody-guided MNase cleavage and purified by phenol-chloroform and ethanol precipitation.  CUT&RUN libraries were prepared as detailed on https://www.protocols.io/view/cut-amp-run-targeted-in-situ-genome-wide-profiling-14egnr4ql5dy/v3. Briefly, end repair and A tailing was performed at 12C for 15min, 37C for 15min, and 58C for 45min in a thermocycler. After Tru-seq adapter ligation, genomics DNA was purified using 2 rounds of Ampure Beads. Libraries were amplified through 14 cycles of PCR amplification and purified using 1.2X volume of Ampure XP beads.  The DNA concentration of each library was quantified using the Qubit and pooled for paired end sequencing on the Illumina Nextseq 2000 or Illumina Nextseq 500.