GSM5277296: zucchini grown in pot soil, with addition of CuSO4 5H2O (320 mg kg-1), for 60 days [pollen CuSO4]; Cucurbita pepo; RNA-Seq
Total RNA was extracted from 0.1 g of fresh plant material using a Sigma-Aldrich Spectrum Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO). TruSeq Stranded mRNA kit (Illumina, San Diego, CA) has been used for library preparation following the manufacturer's instructions. RNA samples were quantified and quality tested by Agilent 2100 Bioanalyzer RNA assay (Agilent Technologies, Santa Clara, CA). Final libraries were checked Agilent Bioanalyzer DNA assay (Agilent Technologies, Santa Clara, CA). Libraries were prepared for sequencing and sequenced on single-end 75 bp mode on NextSeq 500 (Illumina, San Diego, CA).