GSM5051515: PB-CCN1-rep1; Mus musculus; RNA-Seq
500-1000ng total RNA were extracted following the TRIzol® reagent protocol (Cat#15596018, Invitrogen) The mRNA library preparation including mRNA capturing, fragmentation (200-300bp), adaptor (Cat#E7775, NEB) ligation, and library amplification and purification was finished following the manufacturer's protocols of KAPA Hyper Prep Kit (Cat#KK8504, Kapa Biosystems).