GBS of Phaseolus lunatus: sample JMC_1078
For Genotype-By-Sequencing (GBS) library construction, five biological samples of leaf tissue (three young trifoliate leaves of five different plants) were collected for each accession. The samples were frozen and stored at -20 C until processing. DNA isolation was performed from frozen trifoliate leaves using the extraction method developed by Vega-Vela and Chacn Snchez (2011). DNA quality was checked with a Nanodrop 2000 and analyzed in 1% agarose gel electrophoresis; DNA with no visible degradation and a ratio 260/280 above 1.8 was selected. DNA libraries (one per accession) were generated by mixing the DNA of five individuals in equal parts (with the help of a Nanodrop 2000 and electrophoresis calibration with lambda DNA at concentrations of 25, 50 and 100 ng/L), then the DNA restriction for each sample was performed with the ApeKI enzyme and ligated to adapters containing one of 94 unique barcodes for each plate.