16s rDNA sequencing of Rattus norvegicus feces
The V3-V4 region of the 16s rRNA gene was amplified using a primer set (341F ACTCCTACGGGAGGCAGCA/806R CGGACTACHVGGGTWTCTAAT) to analyze the gut microbiota. The raw sequencing data were filtered and optimized using QIIME2 (http://qiime.org/), and the clean data were obtained. Then, Greengenes database (release 13.8) in QIIME2 software was also used to assign operational taxonomic units (OTUs) based on the threshold of 97% sequence similarity.