GSM5963794: WT-2; Mus musculus; RNA-Seq
Total RNA was extracted from  PDGFRA+ cells upon FACS sorting from mouse ventricular tissue from  using the miRNeasy Mini Kit . RNA extracts with an RNA Integrity Number (RIN) value of >8, determined using an Agilent Bioanalyzer RNA chip, were used to construct sequencing libraries. Strand-specific ribosomal RNA depleted sequencing libraries were generated using TruSeq stranded total RNA library preparation kit . The libraries were sequenced as 150bp paired-end reads on an Illumina HiSeq 2500 instrument.