NGS on Drosophila melanogaser cardinal gene: Canton-S strain
The PCR products were separated by agarose gel electrophoresis and extracted from gel using QIAquick Gel extraction kit 250. DNA amplicons were purified using AMPure XP beads (Beckman Coulter, USA) and fragmented using dsDNA Feagmentase (NEB, USA). The fragmentation reaction was carried out in 10 ul volume for 10 minutes at 37C using 25 ng of purified DNA. The fragment-DNA libraries were prepared using TruSeq Nano DNA LT Library Prep Kit (Illumina, USA), following the manufacturers protocol.