GSM5963470: Liver_dRRE_CT2_rep2; Mus musculus; RNA-Seq
Mouse livers from the mutant and WT male mice harvested at six time points throughout the day (CT2, 6, 10, 14, 18, and 22; n=2) were placed in Lysing Matrix D tubes (MP Biomedicals) with TRIzol reagent (Invitrogen), and homogenized by using Fast-Prep 24 instrument (MP Biomedicals). Total RNAs were prepared with the RNeasy Mini Kit (Qiagen), followed by ethanol precipitation. Poly(A)-tailed RNA was isolated from the total RNA by using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, #E7490) and NEBNext Ultra II Directional RNA Library Prep Kit (NEB, #E7760). RNA libraries were prepared for sequencing using standard Illumina protocols