GSM6124772: p18 WT 3; Mus musculus; RNA-Seq
P18 and P20 back skin was dissected and incubated in 0.25% trypsin (Invitrogen 1505-065) for 2hrs at 34C. The epidermal side was mechanically separated and resuspended in 2% FBS PBS. Debris was removed with 40uM and 70uM mesh filters. Whole epidermis samples were processed for bulk RNA-seq. For HFSC bulk RNAseq, cells were labeled with CD45f (eBioscience 12-0495), Ly-6A/E (eBioscience 45-5981-82), CD34 (eBioscience 50-0341-82), and DAPI.  Ly-6A/E- CD34+ CD45f+ HFSC were isolated for bulk RNAseq RNA quality was determined with Aglient Bioanalyzer; only samples with RNA Integrity Number > 8 were used. Library preparation with Illumina TrueSeq library preparation kit Single end Illumina HiSeq 2500 sequencing was performed at the University of California, Irvine High Throughput Genomics Facility. Read alignment was preformed using Kallisto/0.46.2