10X 3' single-cell RNA of BM from precursor stages of multiple myeloma: Sample SMM-7-138P
Frozen BM cells were rapidly thawed, washed, counted and resuspended in PBS and 0.04% bovine serum albumin to a final concentration of 1,000 cells per μl. The Chromium Controller (10x Genomics) was used for parallel sample partitioning and molecular barcoding. To generate a single-cell Gel Bead in EMulsion, cellular suspensions were loaded on a Single Cell 3′ chip together with the Single Cell 3′ Gel Beads, according to the manufacturer's instructions (10x Genomics). scRNA-seq libraries were prepared using the Chromium Single Cell 3′ Library Kit v.2 (10x Genomics). Fourteen cycles were used for the total complementary DNA amplification reaction and for the total sample index PCR.