GSM5917743: RNAP_1.0X_125min_3; Escherichia coli str. K-12 substr. MG1655; RNA-Seq
Total RNA was extracted using RNeasy kit (Qiagen). RNA was treated twice with DNase (Turbo DNA-free kit, Ambion) and quantified using Qubit 2.0 Fluorometer RNA assay (Invitrogen, Carlsbad, CA, USA). Total RNA amounts were determined by gel electrophoresis, using a 1% agarose gel stained with SYBR safe (Invitrogen). RNA was detected using UV with a Chemidoc XRS imager (Biorad). RNA-seq libraries were constructed according to the Illumina's protocol. Library strategy established by GENEWIZ, Inc. (Leipzig, Germany) for RNA-seq