Metabarcoding of sediment samples:BR12
16S RNA gene was amplified using the primer set 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 926R (5-CCGYCAATTYMTTTRAGTTT) and the amplicons sequenced on Illumina MiSeq platform by LGC group (Berlin, Germany) following Earth Microbiome Project protocols (http://www.earthmicrobiome.org/emp-standard-protocols/).