GSM6173225: 2m_ctrl_sup_42 [Single cone cell RNA-seq]; Mus; RNA-Seq
Pieces from superior or inferior zones of retina were dissected from Trb2Cre:Ai6 mice, then dissassociated into suspension cells after brief papain treatment. Fluorescent cells were identified on a Zeiss Axiovert 200M microscope and collected individually using an Eppendorf micromanipulator and suction pipette. Collected single cone cells were transfer into individual PRC tubes for RNA-seq library construction. For ChAP-seq, retinas from ThrbHAB/HAB;Rosa26BirA/BirA and control Rosa26BirA/BirA E18 embryos were cross-linked and affinity-purified with streptavidin to collect the TRb-bound-DNA. For ATAC-seq, cone nuclei were immunopurified from retinas from control (TRb2Cre-het) and TRb2Cre-KO mice on a Rosa26Sun1Gfp/Sun1Gfp background, these purified cone nuclei were treated with Tagment enzyme (using Tagment DNA kit) before DNA purification. Single-cell RNA-seq libraries were constructed by using SMART-Seq v4 Ultra Low Input RNA Kit and ThruPLEX DNA-Seq Kit. For ChAP-seq, the enriched-DNA samples were constructed into sequencing library by using ThruPLEX DNA-Seq Kit. For ATAC-seq,  Tagment-enzyme processed DNA samples were constructed into ATAC-seq library by using Tagment DNA kit.