Tn-seq of X. hortorum pv. vitians LM16734: lettuce replicate 2
Conjugation of X. hortorum pv. vitians LM16734 recipient strain with E. coli MFDpir/pSamEC Himar1 mariner transposon donor strain on TSA 1/10th agar plates. Library was cultivated in 1/10th TSB, then inoculated in lettuce leaves for 10 days before recovery. Subsequent library DNA was prepared using the MmeI protocol.