GSM4743456: RiboSeq_ArfKO_shSCR_replicate_2; Mus musculus; OTHER
RNA was extracted by Trizol and purified using the Zymo Research Direct-Zol RNA Cleanup kit. Ribosome profiling samples were processed as described in https://doi.org/10.1016/j.ymeth.2017.05.028. For RNAseq, total RNA was fragmented by partial alkaline hydrolysis. Fragments between 17 and 34 nt were purified by PAGE. Library construction was performed as described in https://doi.org/10.1016/j.ymeth.2017.05.028.