WXS of human SMM sample: Sample SMM_Study_Sample_44
Tumor DNA was extracted from CD138+ cells from patients' bone marrow. For germline control (normal), DNA was obtained from buccal mucosa (saliva), or peripheral blood mononuclear cells. Genomic DNA was extracted using QIAamp DNA mini kit (QIAGEN) according to the manufacturer's protocols, and double-stranded DNA concentration was quantified using PicoGreen dsDNA Assay kit (Life Technologies). Libraries were prepared by Agilent SureSelect XT2 Target Enrichment kit. To capture the coding regions, we used the SureSelect XT2 V5+UTR capture probes (Agilent). All sequencing was performed on the Illumina HiSeq 4000 platform at the Broad Institute. For tumor only samples (n= 94), libraries were prepared and hybridized using Agilent SureSelect XT2 V5 capture probes (Agilent) and sequenced on Illumina HiSeq 2500 platform.