GSM5983618: WD-PNEC, mock, 96, 32; Homo sapiens; RNA-Seq
RNA was extracted at 48 and 96 h for each condition, in duplicate. Cultures were incubated with 400 µl of Lysis/Binding buffer (High Pure Isolation Kit; Roche) for 5 min at RT. Total Lysed samples were vortexed for 15 sec and transferred to a High Pure filter tube. RNA was extracted as per the manufacturer's instructions RNA sequencing was performed via  Kapa RNA HyperPrep with RiboErase (Roche)