GSM6124679: J774 KO2 UT rep1; Mus musculus; RNA-Seq
RNA from J774 was harvested using Zymo Quick-RNA Miniprep Plus Kit (Zymo #R1504)). All lysates were frozen at -80°C prior to extraction, and all extracted RNA were stored at -80°C as single-use aliquots. Libraries were generated with 200ng or 500ng RNA using KAPA mRNA HyperPrep Kit (Kapa Biosystems #08098123702) according to the manufacturer's instructions. Briefly, single-indexed adapters (Kapa Biosystems #08005699001) or unique dual-indexed adapters (Kapa Biosystems #KK8727) were added to each sample at a final concentration of ? nM. Excess adapter was removed using sequential 0.63X and 0.70X KAPA Pure Bead (KAPA #KK8002) cleanups.