GSM6285106: HTR5-GFP;ino80-5_Nucleosome_rep1; Arabidopsis thaliana; ChIP-Seq
Materials were fixed with 1% formaldehyde and ground with liquid nitrogen into fine powder. After nuclei extraction, mononucleosomes were generated with micrococcal nuclease (MNase) (NEB, M0247S) digestion. Immunoprecipitation was performed with anti-GFP antibody (Thermo Fisher Scientific, A11122). Library was prepared with VAHTS universal DNA library prep kit for illumina (Vazyme, ND607) according to the manufacture's instruction