Genomic sequencing of SB203
Genomic DNA was extracted from overnight cultures, using GenFind v3 reagents (Beckman Coulter, California, USA). . Library prep using Ligation Library Kit (LSK-109, Oxford Nanopore Technologies (ONT), Oxford, UKNT) with native barcoding expansion pack (EXP-NBD104 and NBD114, ONT, Oxford, UK). The library was run on a R9.4.1 MinION flow cell, and was base called with Guppy v3.3.3 using the dna_r9.4.1_450bps_hac (high-accuracy) basecalling model.