GSM6171952: MCF7 cells, COMT, biol rep2; Homo sapiens; RNA-Seq
Total RNA was isolated according to TRI reagent protocol (Sigma-Aldrich, T9424) and its concentration was determined using Qubit RNA BR assay kit (Q10210, Invitrogen). 240 ng of total RNA at 20ng/µl was used in RNA-Seq analysis. The TruSeq Stranded Total RNA LT Sample Prep Kit (Illumina) was used to convert total RNA into a library of template molecules. Library was validated using Bioanalyzer (DNA 1000 Kit, Agilent) and quantified according to manufacturer instructions by qPCR (KAPA Library Quantification Kit Illumina platforms, Kapa Biosystems) using Quant studio (QuantStudio 5, Thermo Fisher Scientific).