16SrRNA-seq of bacteria: women
CV fluid was collected from eligible study participants at two gestational time point. This was with the aid of two sterile Dacron swabs (Deltalab Eurotubo 300263) inserted into the posterior vaginal fornix of each pregnant woman guided by a sterile Cuscos vaginal speculum. CV fluid samples were obtained before any vaginal examination or clinical intervention. The swabs were stored at 20 C immediately after collection and later transferred to 80 C within 1-3 days.