Minion Genome Sequence of Bacillus amyloliquefaciens Group Strain JP3042 Isolated From California Raisin Vineyard Soils
ONT libraries were prepared with Ligation Sequencing Kit (version SQK-LSK109) and Native Barcoding Expansions 1-12 following manufacturers protocols. Genomic DNA was sheared using G-tubes (Covaris) and size selected (>15 kb) using Blue Pippin High Pass Plus (Sage Sciences).  DNA repair/end-prep were performed for 30 min at 20C, then for 30 min at 65C.  Libraries were loaded on a R9.4.1 flow cell for 21-hour runs at 22 fmol and 28 fmol on a MinION sequencer (MIN-101B), with a MinIT v19.05.2 controller running MinKNOW v3.3.2 and Guppy v3.0.3 with high accuracy base calling and Q-score 7 settings.