GSM6068376: CAF1_control; Homo sapiens; RNA-Seq
CFPAC-1 were treated by RSC96 CM or control CM for 72 hours, and CAF1 and CAF2 were treated for 48 hours. After that, CFPAC-1, CAF-1, and CAF-2 were lysed with TRIzol (Invitrogen), and total RNA was extracted using RNeasy Mini Kit (Qiagen, Germany), according to the manufacturer's instructions. RNA sequencing libraries were generated using KAPA RNA library preparation kit (Illumina) and the KAPA dual- indexed adapter kit (Illumina) for CAFs, and NEBNext® Ultra™ RNA Library Prep Kit (Illumina) for CAFPAC-1. Sequencing was performed on an Illumina HiSeq X10 platform (2 × 150 bp). RNA libraries were prepared for sequencing using standard Illumina protocols