GSM5917641: 6D_6H_High Dose [A7-44]; Pimephales promelas; RNA-Seq
Larvae were removed from exposure vessels, placed into Eppendorf tubes together with a steel bead and frozen in liquid nitrogen.  MagMax lysing/binding buffer (100 μl) was added to each tube. Libraries were prepared from 250 ng RNA using the SENSE mRNA-Seq Library Prep Kit V2 (Lexogen, Greenland, NH) according to the supplied protocol.