Partial 18S rRNA ampified from root hairs of Eriosema cordatum
PCR amplicons were purified using the Agent Court AMPure XP beads (Beckman Coulter, Brea, CA, United States). The libraries were amplified with a limited-cycle PCR program (12 cycles) to add the index 1 (i7) and index 2 (i5) adapters, containing sequences required for cluster generation of the Illumina flow cell. The quality and sizes of the resulting DNA fragments were evaluated on a 1.5% (w/v) agarose gel. The libraries were quantified with a fluorometric method (Qubit, Life Technologies) and normalized to 4 nM using a standard dilution method. Subsequently, the amplicon libraries were pooled, and paired-end sequencing was done on an Illumina MiSeq platform using a MiSeq Reagent Kit V3 600 cycles (Illumina, San Diego, CA, United States).