GSM5848489: 4-month-old brain organoids from feeder-free iPSCs with PD173074 treatment; Homo sapiens; RNA-Seq
Four-month-old organoids were dissociated into a single-cell suspension using TrypLE select. The dissociated cells were resuspended in ice-cold PBS containing 0.04% BSA (Miltenyi Biotec), supplemented with 0.1% Propidium Iodide (Sigma) for removing dead cells, and sorted with a flow cytometer SH800 (SONY). Sorted cells were loaded onto a Chromium Single Cell 3′ Chip (10x Genomics) and processed through the Chromium controller to generate single-cell gel beads in emulsion. The libraries were prepared with the Chromium Single Cell 3′ Library & Gel Bead Kit v3 (10x Genomics).