GSM6125798: SJRH30 TLE3KO replicate 2 [266]; Homo sapiens; RNA-Seq
Total RNA extracted using RNEasy mini kit (Qiagen) or Zymo research RNA kit. RNA quality was assessed using Tapestation (Agilent) and quantified using Qubit (ThermoFisher Scientific). 250ng of Total RNA was used to enrich the mRNA using NEBNext Poly (A) mRNA magnetic isolation module (Catalog: E7490, New England Biolabs) by following the manufacturers' protocol. The enriched mRNAs were further taken for the library preparation using the NEBNext Ultra II RNA Library Prep Kit for Illumina (Catalog: E7775S, New England Biolabs).