GSM5117453: Interacting S.co ΔcvnD8 -rep3 (2020); Streptomyces coelicolor; RNA-Seq
Biomass was scraped off agar plates and frozen in liquid nitrogen followed by grinding with mortar and pestle and addition of trizol. The Zymogen Direct-zol RNA miniprep kit (R2051) was used to purify the RNA, with the optional DNase treatment step included RNA preparation was done by the UC Berkeley Functional Genomics Laboratory. Briefly, rRNA was removed using the Illumina Ribo-Zero rRNA removal kit (bacteria) and the remaining RNA was prepared with a Wafergen PrepX RNA library prep kit for Illumina. Indexing PCR was performed using in-house dual indexed primers and 14 cycles.