GSM5009771: CPZKO2_62; Mus musculus; RNA-Seq
Total RNA was extracted from ventricular embryonic tissue collected from embryonic hearts by removing atria and valve region using QIAzol Lysis Reagent (Qiagen; CA; USA) and miRNeasy Mini Kit (Qiagen; CA; USA) RNA was quantified and purity checked using a NanoDrop ND-1000 (Thermo Scientific; MA; USA). RNA integrity was verified using an Agilent 2100 Bioanalyzer (Agilent Technologies; CA; USA). 500 ng of total RNA were used with the TruSeq RNA Sample Preparation v2 Kit (Illumina; CA; USA) to construct index-tagged cDNA libraries. RNASeq libraries were sequenced (single-end mode and length of 75 bp) on the Genome Analyzer IIx system using the standard RNA sequencing protocol in the TruSeq SBS kit v5.