GSM5827288: KLF4-KO-RNA-seq-Rep1; Mus musculus; RNA-Seq
For RNA -seq: cells were lysed with Trizol reagent (Life Technologies) and RNA was extracted according to the manufacturer's instructions; For HiChIP libraries, DNA was extracted according to the manufacturer's instructionsQIAseq FX DNA Library Kits were used to generate the sequencing library. HiChIP libraries were size-selected to 300–700 bp using AMPure XP beads (Beckman) and subjected to 2 × 150-bp paired-end sequencing on HiSeq XTen (Illumina). Two biological replicates were analyzed for each experimental condition. RNA libraries and HiChIP libraries were prepared for sequencing using standard Illumina protocols