GSM5658033: KO_C2; Homo sapiens; RNA-Seq
Cells were lysed with TRIzol (Life Tech, Cat. #15596026), followed by RNA extraction with chloroform following the standard protocol and RNA purification using RNeasy Mini Kit (QIAGEN, Cat. #74104). Experiment was performed in triplicate.  Libraries were made using an Illumina TruSeq stranded mRNA kit.