GSM5827250: P60Hearing3; Rattus norvegicus; RNA-Seq
Cochleae were collected and removed from the temporal bone. Spiral ganglia were via microdissection by removing the bony tissue, spiral ligament, and organ of Corti. Ganglia were placed into RNAlater and flash frozen prior to RNA extraction. At the time of RNA extraction, the RNAlater was removed and replaced by Trizol and the tissue homogenized. The aqueous fraction was isolated using chloroform and phase lock tubes. RNA was then purified from the aqueous fraction using the RNeasy Kit (Qiagen) with on-column DNase treatment following manufacturer protocol. Libraries were prepared using the Illumina Ribo-zero Gold Epidemiology library preparation kit