ORF50_72_73_OriLyt2_73g1
Genomic DNA isolated from edited or control cells were used as template to amplify a fragment of ~650-780 bp using Q5 polymerase (NEB). The PCR products were then purified, mixed according to the description in the title section and diluted to 1 ng/mL for fragmentation and ligation to Index using Nextera XT DNA Library kit (Illumina), then purified with agarose beads. The quantity and fragment range of the samples was verified by Bioanalyzer before 2X75 bp sequencing with NextSeq 500 in the Genomics Core Facility in Stony Brook University.