NHP_927
Amplicon library synthesis was done on the Fluidigm Access Array System. A mastermix for amplification was prepared using the Roche High Fidelity Fast Start Kit and 20x Access Array loading reagent according to Fluidigm protocols. All libraries were quantified on a Qubit fluorimeter and were run on a Fragment Analyzer. Samples were then pooled in equal amounts, denatured and spiked with 20% non-indexed PhiX control library provided by Illumina and sequenced on an Illumina MiSeq 250 bp paired-end sequence flowcell.