GridION Sequencing of Hanleya hanleyi gDNA
To produce long-read data via Oxford Nanopore sequencing, genomic DNA was extracted with an EZNA Tissue DNA Kit (Omega Bio-tek) and was cleaned and enriched for higher-molecular-weight fragments with the Short-Read Eliminator kit (Circulomics) according to the manufacturers instructions. Three sequencing libraries were prepared with the LSK-109 ligation-based library preparation kit and sequenced them on three R9.4.1RevD flow cells. Reads were base called with Guppy 4.0 and trimmed with PoreChop (Wick 2018) with the --discard_middle flag.