GSM6068272: hs_GF_ASC_hnRNPA2B1_Sub2; Homo sapiens; ChIP-Seq
Fat biospsies were collected and SVF were isolated by centrifugation. Lysates were clarified from sonicated nuclei and hnRNPA2B1-chromatin complexes were isolated with antibody (Novus Biologicals NB120-6102).  DNA was purified with MinElute PCR Purification Kit (Qiagen). ChIP-seq  libraries were prepared and sequenced using standard Illumina protocols for NovaSeq 6000 instrument with PE150.