16S rna seq of Wistar rat: gut microbiome
DNA was isolated from rat feces using the QIAamp DNA Stool Mini kit (51504, QIAGEN) with modification described in IHMS_SOP 006 V2. 16s rRNA gene variable region 4 libraries were prepared using 1 round of PCR with primers F515 (5GTGBCAGCMGCCGCGGTAA-3) and 806R (5-GGACTACHVGGGTWTCTAAT-3), modified to contain Illumina adapter sequences.