RNA-Seq of Forficula auricularia: juvenile instar 1-2
Total RNA was extracted using Direct-zol RNA MicroPrep kit (Zymo Research) with an on filter DNAse treatment following the manufacturers instructions. TruSeq stranded mRNA libraries were prepared and sequenced as 2x100bp paired-end reads across two lanes of HiSeq 2500 Rapid V2 flowcell.