RNA-Seq of DUC18828, replicate 2
PDC-DUC18828 (1x106 cells) cultures, in triplicate, were dissociated and resuspended in cell media, centrifuged and washed in PBS, and pelleted by centrifugation. RNA was extracted using Qiagen RNeasy Kits (Cat #74106) according to the manufacturers protocol. Sequencing libraries were prepared from purified RNA using the Kapa Stranded mRNA-seq kit (Roche, Cat #KR0960  v6.17). The three libraries  were pooled and sequenced on a single lane of a NovaSeq 6000 SP flow cell, to generate 50bp paired-end reads.