NextSeq 500 paired end sequencing; ATACseq of TNG-A derived mESCs comparing WT to Dax1, Nr5a2 and Dax1/Nr5a2 knock-out clones
ATACseq of TNG-A derived mESCs comparing WT to Dax1, Nr5a2 and Dax1/Nr5a2 knock-out clones
According to the published protocol (Buenrostro 2015, PMID: 25559105) Cells grown in 2iLIF on gelatin coated plates and transferred to SerumLIF 1nM Retinoic Acid on gelatin for 48h G6C18 mouse embryonic stem cells, transfected with gRNAs AGAGCCGACCGCATGCGAGG and AAAAGTTCCAGTATCAGGTG, mutant clone According to the published protocol (Buenrostro 2015, PMID: 25559105) According to the published protocol (Buenrostro 2015, PMID: 25559105)