HY-30-F1
mRNA with poly(A) were isolated from the total RNA using oligo (dT) beads, purified, fragmented (100 bp~402 bp) with an ultra-sonicator and reverse transcribed into first strand cDNA using random primers. Subsequently, sequencing adapters were connected the short fragments, and the resultant cDNA libraries used for paired-end RNA-seq. After the sample is tested, the eukaryotic mRNA is enriched with magnetic beads with Oligo (dT).