Abrevis_pool
Parasitized colonies of the ant T. nylanderi were collected in the years 2013 - 2015 in the Lenneberg forest and Eltville near Mainz, Germany. In January 2016, 40 infected workers from 18 different colonies were dissected, resulting in a total of 329 isolated cysticercoids that were washed three times in DNase-free water to remove remains of ant tissue. RNA extractions were performed in four rounds so that dissections of cestodes for one round did not last longer than one hour (see Supplementary Information Table S1). Cestodes per round were transferred into 100 l of Trizol and RNA extraction was performed using the RNAeasy mini extraction kit (Qiagen) and standard protocol. Afterward, samples were combined and sent to BGI Hongkong for RNA sequencing on an Illumina HiSeq 4000.