GSM5765294: Cut&Run_Nrf2_Dpf2f/f_LK_Exp6; Mus musculus; OTHER
Lin- cKit+ cells were obtained from bone marrow of 28-days old Dpf2f/f and Dpf2D/D mice. Approximately 0.5 million cells/sample were crosslinked (1 min, 1% formaldehyde), quenched (125mM Glycine) and washed in buffers following the CUTANA ChIC/CUT&RUN kit according to the manufacturer's CUT&RUN cross-linking protocol (EpiCypher, 14-1048). 0.5 million cells were used per IP with 1 ug of antibody. Cut&Run libraries were generated using the NEBNext Ultra II DNA library Prep kit for Illumina (NEB, E7370L)