GSM5984063: 3D gastruloids Budesonide_rep2 [B2]; Mus musculus; RNA-Seq
Total RNA was extracted using the RNAeasy kit. RNA libraries were prepared for sequencing using standard Illumina protocols. The quality of RNA were evaluated by NanoDrop One spectrophotometer (NanoDrop) and by TapeStation 4200 (Agilent Technologies  ), respectively. Indexed libraries were prepared from 1 µg/ea purified RNA with TruSeq Stranded mRNA (Illumina) Li-brary Prep Kit according to the manufacturer's instructions.