GSM4962575: Laser microdissection REF9; Homo sapiens; RNA-Seq
Kidney tissue was obtained from one of two sources. The first is a clinical kidney biopsy, placed in Michels solution and frozen on OCT within 24 h. The second is either a deceased donot nephrectomy or unaffected portion of a tumor nephrectomy. Approximately 1 million square microns of cross-sectional glomerular biopsy tissue was cut on a cryotome and placed in Arcturus Picopure extraction buffer. RNA was extracted according to the Arcturus protocol. SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input RNA sequencing on an Illumina HiSeq 4000, 100 millions input reads per sample