GSM4744848: old control rep#1; Mus musculus; RNA-Seq
Mice were euthanised by spinal dislocation followed by the dissection of the spinal bones and both femurs, tibias and iliacs. Bones were crushed using a pestle and mortar and cells were collected in  ice-cold PBS+2%FBS, filtered and washed. Bone marrow cells were c-kit enriched and live SLAM-HSCs (Lin- Ckit+ Sca1+ CD48- CD150+) were FACS sorted into trizol for RNA isolation. Sequencing libraries were produced using Nextera XT DNA Preparation Kit (Illumina) following a modified version of the manufacturer's protocol with 20-fold volume reduction