RNA-Seq of homo sapiens: Hase
The constructed libraries were amplified with phi29 to make DNA nanoball (DNB), which had more than 300 copies of one molecular. The DNBs were loaded into the patterned nanoarray and single-end 50 bases reads were generated in the way of combinatorial Probe Anchor Synthesis (cPAS). We applied Bowtie2 for mapping the clean reads to the reference gene sequence, and then used RSEM to calculate the gene expression level of each sample.