GSM5525279: TMZ-resistant U87MG_SiHDAC6; Homo sapiens; RNA-Seq
Total RNA was extracted using Trizol® Reagent (Invitrogen, USA) according to the instruction manual. Purified RNA was quantified at OD260nm by using a ND-1000 spectrophotometer (Nanodrop Technology,USA) and qualitated by using a Bioanalyzer 2100 (Agilent Technology, USA) with RNA 6000 LabChip kit (Agilent Technologies, USA). All RNA Sample preparation procedures were carried out according to the official Illumina protocol.