GSM5525114: draculin:mCherry+;gata1:GFP- at 30 hpf #2 (replicate); Danio rerio; RNA-Seq
draculin:mCherry+;gata1:GFP- or runx1:mCherry+ cells were isolated from transgenic fluorescent zebrafish embryos/larvae using FACS Aria. Sorted Cell populations were loaded on a Chromium Single Cell Controller (10X Genomics) to generate single-cell gel beads in emulsion (GEMs) by using  Single Cell 3' Reagents Kits v3.1 (10X Genomics). Captured cells were lysed and the released RNA wer barcoded through reverse transcription in individual GEMs (Zheng et al., 2017). ScRNA-Seq libraries were prepared using Single Cell 3' library Gel Bead Kit v3.1 (10X Genomics) following the manufacture's instructions.