GSM6068155: P2-CM Control-Rep2; Mus musculus; RNA-Seq
For RNA isolation from P1/P2 CM, cells were harvested, washed in PBS  lysed using 350ul of RLT-lysis budder as per the manufacturer's protocol (RNAeasy Kit Qiagen). RNA isolation was performed as per the protocol, followed by on-column DNA digestion to remove any traces of DNA as per the instructions. Libraries were created using 40 unique dual-indexed (UDI) TruSeq stranded mRNA libraries for the project.