Hi-C raw data of Pseudobagrus ussuriensis genome sequencing
The Hi-C library was prepared according to a previously reported procedure with slight modifications. Firstly, approximately 100 mg of muscle sample was shattered and treated with nuclei isolation buffer supplemented with 2% formaldehyde to induce crosslinking. Secondly, 2.5 M glycine was added to stop the crosslinking reaction. After purification, the nuclei were digested using 100 units of DpnII restriction enzyme and marked with biotin-14-dCTP, and then ligated with T4 DNA Ligase. The ligated DNA was sheared into fragments with a size range of 300600 bp, and then these fragments were blunt-end repaired and A-tailed, followed by purification through biotin-streptavidin-mediated pull down. Finally, after quantification, the Hi-C library was sequenced using an Illumina Hiseq X Ten platform (Illumina, USA) at NextOmics Bioscience Co., Ltd (Wuhan, China) though 150 bp paired-end sequencing.