COR
mRNA was enriched from a pool of total RNA using Oligo-dT enriched magnetic beads (Vazyme Biotech Company, Jiangsu, China). The RNA was sheared into small pieces using an RNA fragmentation kit according to the manufacturers instructions (Illumina, San Diego, CA, USA). The fragments were reverse transcribed into single stranded cDNA with random N6 primers followed by second strand cDNA synthesis. The cDNA fragments were subjected to end repair and ligated with adapters, followed by PCR amplification. The amplified products were used to generate single-stranded cDNA libraries. cDNA sequencing was carried out on a BGISEQ-500 platform from Huada Gene Technology Co., Ltd