GSM5765252: RNAseq_SKNO1_Rep2; Homo sapiens; RNA-Seq
total RNA was obtained using the RNEasy Plus micro kit (Qiagen, 74034). Ribosomal RNA vas removed using the NEBNext rRNA Depletion kit (New England Biolabs #E6310) following manufacturer's protocols, and RNA-Seq libraries were prepared using the Illumina's TruSeq Total-stranded RNA-seq prep kit (NuGEN Technologies). RNA-Seq libraries were sequenced on an Illumina NextSeq 500 High-Output kit platform (paired-end, 75 bp reads) to obtain ~40 M reads/sample. RNAseq libraries were prepared for sequencing using the Illumina's TruSeq Total-stranded RNA-seq prep kit (NuGEN Technologies).