GSM4743276: sc-RNAseq_CD34+ cells - 72h_lane7_donor2; Homo sapiens; RNA-Seq
Single cells were marked with 7AAD to remove dead cell by FACS sorting. Cells were individually sorted into 96-well plates containing 4µL of lysis buffer (using Triton 100% as lysis reagent) Supplemented with RNase inhibitor (RNaseOUT, Thermo scientific) and specific barcoded RT primers. Single-cell RNA sequencing was performed as described in "Jaitin et al.Science, 2014 Feb 14; 343(6172): 776–779" with minor adaptations.