GSM4743730: Li13650: CkoWt236; Mus musculus; RNA-Seq
After distention with collagenase P (0.66mg/ml in Hank's Balanced Salt Solution, 2.5ml/pancreas) the pancreas was removed, agitated at 37 degrees celcius for 15 min and then islets were hand-picked. Total RNA was isolated with RNeasy Mini Kit (Qiagen) from 100–200 islets/mouse. Library preparation was completed with NEBNext®Poly(A) mRNA Magnetic Isolation Module and NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina by the UCDNA Services, Cumming School of Medicine, University of Calgary