GSM6124615: H3K79me3 TOPmentation in DMSO-treated SEM cells with SSRP1 endogenously tagged with FKBP12(F36V); Homo sapiens; ChIP-Seq
Cells were washed with PBS and fixed in either 1% FA for 10 minutes (histones and HT-CM) or 2mM DSG for 30 minutes and 1% FA for 30 minutes (TFs). Cells were sonicated to give fragments of 100-300bp followed by IP. IPed chromatin was tagmented by incubating in 29 µl Tagmentation Buffer (10 mM Tris-HCl pH 8.0, 5 mM MgCl2, 10% dimethylformamide) and adding 1 µl of transposase (Illumina) at 37 ˚C for 10 min. Tagmented DNA was amplified and indexed with NEBNext Ultra II Q5 Master Mix (NEB).