WGS of Cryptoccous
Sequencing library is generated using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, England) and following manufactures recommendations. The genomic DNA is randomly fragmented to a size of 350bp by Bioruptor, then DNA fragments are narrowly size selected with sample purification beads. The selected fragments are then end polished, A-tailed, and ligated with the full-length adapter. After these treatments, these fragments are filtered with beads again. At last, the library is analyzed for size distribution by Agilent2100 Bioanalyzer and quantified using real-time PCR.