GSM5625407: BL160124N; Homo sapiens; RNA-Seq
The control tissue is the adjacent non-cancerous bladder tissue in the same patient. After collection, all samples were washed with pre-cooled RNA-free PBS (Thermo, USA), then placed in RNAlater™ Stabilization Solution (Thermo, USA), and stored at -80°C.Use MagMAX™ mirVana™ Total RNA Isolation Kit (Thermo, USA) to extract total RNA from the sample. Subsequently, Ribo-Zero Magnetic kit (Epicentre, USA) and magnetic stand (Thermo, USA) were used to remove ribosomal RNA (rRNA) from the total RNA sample. the library was constructed using the Illumina TruseqTM RNA sample prep Kit method (Illumina, USA). Finally, the library was sequenced on Novaseq 6000 SBS Kit v3-HS (200cycles) (Illumina, USA)