GSM5848534: div0-Rep2; Mus musculus; RNA-Seq
Total RNA from 1x10^6 _Phase-I_ and 5x10^5 _Phase-II_ cells was isolated using Qiazol _Qiagen_ kit according to manufacturer's protocol. RNA-sequencing of total RNA was performed at the Johns Hopkins Deep Sequencing and Microarray Core facility using standard protocol for NextSeq 500 sequencer. Ribosomal RNA was depleted from 100ng of total RNA and barcoded library was made and 50bp single end reads were generated from each sample.