GSM5848628: Vehicle Sham 3; Mus musculus; RNA-Seq
Each muscle was homogenized in 700 uL Qiazol. Muscle homogenate was then transfered into Phase Lock Gel Heavy tubes with 150 uL of BCP and were mixed for one minute followed by 3 minutes on the benchtop. Samples were then centrifuged at 12,000xg for 15 minutes at 4C and then RNA was isolated using the Qiagen miRNeasy Mini Kit. RNA concentration was assessed via Nanodrop and the RIN was determined using an Agilent 2100 bioanalyzer. Standard Eukaryotic RNA-Seq (cDNA library) prep from Novogene