GSM5525338: ciPS_C6_d14_scRNA; Pan troglodytes; RNA-Seq
HS1 and PT1 were differentiated to day 14, washed twice with DPBS, and dissociated with Accutase for approximately 10 minutes, followed by centrifugation at 400 × g for 5 minutes in wash medium. All pipetting was done very gently to avoid cell death. The pellets were resuspended in 1 ml PBS with 0.04% BSA (Sigma) and filtered through 100 µm cell strainers (Falcon) twice. Cells were resuspended in order to yield a concentration of approximately 1000 cells/µl. The single-cell libraries were prepared with Chromium Single Cell A chip kit and Chromium Single Cell 3' Library & Gel Bead kit v2 (10× Genomics), quality controlled, and quantified using Qubit ds DNA HS and Bioanalyzer High Sensitivity DNA Assay prior to sequencing. The samples were sequenced for 26 cycles on read 1 and 98 cycles on read 2 using the Illumina NextSeq 500.