GSM4743023: S1000-3; Caenorhabditis elegans; RNA-Seq
Cell-type spedific polysome complexes were immunoprecipitated using FLAG conjugated magnetic beads. Immunocomplexes were eluted from beads using the FLAG peptide and mRNA purified using TRIzol reagent (Invitrogen). RNA libraries were prepared for sequencing using standard Illumina protocols adapted for low input samples.