GSM5860371: Male Vehicle Mouse S10; Mus musculus; RNA-Seq
Perilesional brain tissue was removed, flash frozen in liquid nitrogen. Total RNA was isolated using RNeasy plus micro kit (Qiagen). Quantity of total RNA was assessed with the Qubit 2.0 and quality was checked using a RNA 6000 Nano chip on Agilent's bioanalyzer. Barcoded mRNA seq libraries were prepared from 400 ng total RNA using a NEBNext® Poly(A) mRNA Magnetic Isolation Module and NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® according to the manual with a final amplification of 12 cycles. Concentration was determined using Invitrogen's Qubit HS assay and fragment size was analyzed on Agilent's 2100 Bioanalyzer on a HS DNA chip.