GSM5939631: CTRL_LSK1; Mus musculus; RNA-Seq
4 days after first poly I:C injection, 100,000 of LSK cells from Myh9fl/fl;Mx1-cre or littermate Myh9;no cre mice were sorted separately into 500 µL DPBS-BSA buffer (0.5% BSA) using a 1.5 mL Eppendorf tube. After a single wash, RNA was harvested using Trizol reagent. Illumina Truseq RNA Sample Prep kit (Cat#FC-122-1001) was used with 1ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequecing using standard Illumina protocols.