GSM4744766: MS1 cells expressing TAZ 4A Rep 1; Mus musculus; RNA-Seq
RNA extracted with RNAeasy plus mini kit. RNA was DNAse treated with the Invitrogen Turbo DNA-Free Kit Libraries were prepared with the Illumina TruSeq Stranded Total RNA LT Sample Prep Kit. Poly-A RNA was purified and fragmented before cDNA synthesis. Adapters were ligated, and then samples were PCR amplified and purified with Ampure XP beads. Samples were normalized, pooled, and run on an Illumina HiSeq 2500 using SBS v3 reagents