GSM5963444: HepG2 cells - 6N treatment - replicate 3; Homo sapiens; RNA-Seq
Cells were washed with PBS, extracted in RLT buffer, and stored at -80°C until use. Amplified cDNA was fragmented and appended with dual-indexed bar codes using the NexteraXT DNA Library Preparation kit (Illumina). Libraries were validated by capillary electrophoresis on a TapeStation 4200 (Agilent), pooled at equimolar concentrations, and sequenced on an Illumina NovaSeq6000 at 100SR, yielding ~35 million reads per sample.