PacBio sequencing of environmental Legionella sp.  PC997
Pacific Biosciences 20 kb library. Sheared. DNA damage/end repair. SMRTbell ligation (template kit 1.0 reagents). BluePippin size selection. PacBio RSII instrument; P3-C3 chemistry with DNA sequencing kit 4.0 v2 and SMRT cell v3.