Surgical face masks as a new source of emergent pollutants in aquatic systems: analysis of their degradation product effects in Danio rerio through RNA-Seq.
Total RNA was isolated using the Maxwell 16LEV Simply RNA Tissue Kit (Promega) according to the manufacturer`s instructions. The quantity of RNA was measured in a NanoDrop ND-1000 (NanoDrop Technologies, Inc.), and RNA integrity was analysed in an Agilent 2100 Bioanalyzer (Agilent Technologies Inc.) according to the manufacturer's instructions. All the samples passed the quality control tests and were used for Illumina library preparation. Three biological replicates per treatment (control, PDM, HDM and W-HDM), composed of each replicate by 7 pooled larvae, were used for mRNA