GSM5984160: D- mouse 57 - Ecad low; Mus musculus; RNA-Seq
FACS sorted samples were frozen at -80C in FACS collection medium and Trizol LS reagent (Invitrogen, Cat. No 10296010). RNA was harvested with Trizol LS reagent according to the manufacturer´s protocol. To help with the visualization of the pellet we used GlycoBlue Coprecipitant (Invitrogen, Cat. No AM9516) as described by the manufacturer's protocol. RNA libraries were prepared for sequencing using standard Illumina protocols