GSM4744792: K562_dp300_total RNA seq; Homo sapiens; RNA-Seq
Total RNA was extracted using RNeasy Plus Mini Kit (Qiagen) according to the manufacturer's instructions and qualified by Qubit Fluorometer (RQN > 7) Ribosomal RNA (rRNA) from extracted RNA was depleted using NEBNext rRNA Depletion Kit (New England Biolabs #E6350L). rRNA-depleted RNA was reversely transcribed using NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs #E7765S) as suggested by the manufacturer.