RNAseq of sample pYES_BRCA_Anew_pH3-52066
Directional polyA+ RNA libraries were prepared from 1 g of total RNA using the SureSelect Strand-Specific RNA Library Prep for Illumina Multiplexed Sequencing (Agilent Technologies) according to the manufacturers protocol. Libraries were qualitatively checked on the Agilent 2100 platform and quantified on the Nano-Drop 3300 Fluorospectrometer (Thermo-Scientific) using the Quant-IT PicoGreen assay kit (Life-Technologies). Equimolar quantities of cDNA libraries were pooled and subjected to 2  120 bp paired-end sequencing on the NextSeq 500 platform (Illumina).