GSM5799135: sample_109; Salmo salar; RNA-Seq
RNeasy Plus Mini Kit (Qiagen, Crawley, UK) was used according to supplier's protocol to extract and purify total RNA from the cell lysate. RNA samples (2 µg) were sent to the Norwegian Sequencing Centre (NSC) (www.sequencing.uio.no) in Oslo, Norway, where the sequencing and library preparation were performed. DNA libraries were prepared using 90 ng total RNA input to the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, California, USA) per manufacturer's protocol. For multiplexing, standard Illumina adaptors were used. The libraries were sequenced using the NextSeq Illumina platform (Illumina, San Diego, California, USA) according to the manufacturer's instructions, generating single end 75 bp read libraries with an average library size of 10±2 million reads