GSM6172915: HisC, 6.5-7h, STRIPEseq, rep4; Drosophila melanogaster; OTHER
Total mRNA was extracted using fresh or with  RNAlater (Invitrogen) preserved (at -80°C) embryos. mRNA is extracted using Qiagen RNeasy Plus Micro kit adjusting the protocol for small RNA species as described by the manufacturer 200ng total input RNA was used. Uncapped mRNA was digested by Terminator Exonuclease. Adapter and indices were added by template switching oligo PCR, the final library was amplified by PCR using NEB Ultra II Q5 PCR master mix M0544S (14 cycles) following the protocol published by  Policastro et al., 2020 STRIPE-seq, Policastro et al., 2020