GSM5963135: circSOD2-sh treated-2; Homo sapiens; miRNA-Seq
According to the structure characteristics of small RNA with 3' -end hydroxyl group and 5' -end phosphoric acid group, the 8 RNA samples of this project were processed by 3' -end connection, reverse transcription primer hybridization, 5' -end connector connection, cDNA single-strand synthesis and library amplification using the catalytic reaction characteristics of related enzymes and molecular biology techniques.After quality inspection and purification, libraries that meet Illumina platform sequencing were finally obtained. RNA libraries were prepared for sequencing using standard Illumina protocols