CH1949WGS
Briefly, the total genomic DNA (gDNA) was extracted using QIAamp DNA minikit and QIAcube (Qiagen) according to the manufacturers instructions, and the WGS was done using the Illumina MiSeq instrument (Illumina, San Diego, CA, USA) using the Nextera sample preparation method with 2  150 bp paired end reads. De novo genome assembly was performed using the CLC Genomic Workbench (version 20.0.4; CLC Bio, Aarhus, Denmark), and contigs with a minimum contig length of 800nucleotides (nt) were generated. The resulting assembled sequences were analyzed using ResFinder 4.1 (for antimicrobial resistance genes), MLST 2.0 (for Multilocus sequence typing (MLST) analysis on the Center for Genomic Epidemiology server (http://www.genomicepidemiology.org/).