nanopore sequencing from SD-ZI125 Drosophila melanogaster adults
We extracted high molecular weight DNA from ~200 frozen female SD-ZI125 homozygous virgins using a standard phenol-chloroform method and spooled DNA on capillary tubes. We constructed a library with ~1ug DNA using RAD004 kit and an ultra-long read sequencing protocol (Quick). We sequence the library using R9.4 flow cells and called bases with ONT Albacore Sequencing pipeline software version v2.2.10.