GSM6124708: W-Pe4; Drosophila melanogaster; RNA-Seq
Adult flies were snap frozen on dry ice in groups of 5. Total RNA was then isolated using Trizol according to the manufacturer's instructions (Invitrogen; 15596-018). Extracted RNA was then DNase treated (Ambion; 2238G) to be used for subsequent qPCR or mRNA sequencing. RNA libraries were prepared for sequencing using the Ultra II Directional RNA library kit (New England BioLabs) according the manufacturer's instructions