GSM5737732: 3d_M1; Pseudomonas syringae pv. tomato str. DC3000; RNA-Seq
After 5hrs of infection, Leaves from each treatment (“M” or “F”)  were vacuum infiltrated with a RNAlater-like RNA stabilizing buffer pH 5.2 at 95 kPa for 2 min followed by a slow release of the vacuum.  Then they were frozen in liquid nitrogen until RNA extraction. RNA was extracted by grinding under frozen conditions in Trizol then purified using either the Zymo RNA miniprep kit, or the NEB Monarch RNA isolation kit. All samples were DNAse- treated using the Ambion TurboDNAse kit, then cleaned up with the NEB RNA Cleanup kit. RNA transcriptome libraries were prepared with the Illumina Tru-Seq stranded total RNA kit as per instructions, using the rRNA-Removal Beads for both bacteria and plants (or just bacteria in the case of P-samples) to remove ribosomal RNAs. Enriched and amplified libraries were given to the GGBC for quantification, QC analysis and RNA sequencing. Samples were divided into two runs on two different dates. All runs were performed on the Illumina NextSeq sequencer using three Single-End 75-High Flowcells