RNA-Seq of untreated CCL-19 induced resting CD4+ T cell model of HIV-1 latency
PolyA RNA was purified from total RNA, followed by fragmentation and cDNA synthesis. Libraries were adapter ligated and amplified. HIV-1 specific sequences were enriched by the use of a custom designed probe library (Agilent SureSelect). Post-capture PCR indexing of samples were done to allow for multiplexed sequencing. Cluster amplification and preparation of templates for sequencing were done according to Illumina specifications.