GSM6124306: COV15; Homo sapiens; RNA-Seq
All endometrial biopsies were collected by endometrial aspiration using an intrauterine cannula (Cornier pipelle) and preserved in RNA Later (Invitrogen, ref. AM7021) until cryopreservation at -80ºC. Total RNA was extracted from the thawed endometrial biopsies using the miRNeasy Mini Kit (Qiagen, ref. 1038703), according to the instructions of the RNeasy FFPE Handbook protocol. The amount of total RNA isolated from the 24 tissues was quantifed with NanoDropTM One spectrophotometer (ThermoFisher) while RNA integrity was assessed using the Fragment Analyzer System (Agilent). Sequencing libraries were constructed using the TruSeq stranded mRNA protocol (Illumina) following the manufacturer's instructions.