Bisulfite-Seq of tomato: two weeks' seedling roots
Genomic DNA was extracted from tomato roots using DNeasy Plant Mini Kit (Qiagen). After checking DNA integrity by agarose-gel electrophoresis, bisulfite conversion was performed using the EZ DNA methylation-Gold Kit (Zymo Research) according to the manufacturers instructions. The library was constructed by Novogene Corporation (Beijing, China). Subsequently, pair-end sequencing of sample was performed on Illumina platform (Illumina, CA, USA). Library quality was assessed on the Agilent Bioanalyzer 2100 system.