GSM5116226: 2_WT_hmC_mm10; Mus musculus; MeDIP-Seq
Genomic DNA from murine epithelial preps was purified, sonicated, and then ligated to Illumina barcode adaptors.  Ligated DNA was denatured and incubated with a-5hmC antibody (#39769, 1:2,000; Active Motif, Carlsbad, CA) overnight at 4oC, and then immunoprecipitated DNA was purified Immunoprecipitated DNA was sequenced using standard Illumina protocols. Libraries were sequenced on the Illumina NextSeq 500 following the manufacturer's protocols.