GSM6124422: N1 for sncRNA-seq; Homo sapiens; ncRNA-Seq
For small RNA-seq profiling, the plasma exosome RNA was purified from 13 subjects, respectively. The exosome RNA was isolated from plasma exosome using the RNA isolation reagent TRIzol (Invitrogen). By using the QIAseq® miRNA Library Kit (QIAgen, Cat# 331505), small RNA-seq libraries were constructed with 2~10 ng of the prepared exosomal small RNA in line with the manufacturer's protocols. Small RNA-seq libraries were prepared according to Illumina's instructions and sequenced on an Illumina 3000 analyzer by EPIBIOTEK Company (Guangzhou, China).