GSM5827451: KO-2  (Single cells KO vs. WT SMC); Mus musculus; RNA-Seq
Ascending aorta/aortic arch segments were enzymatically digested under cold conditions, single (based on SSC-W vs SSC-H and FSC-W vs FSC-H gating), viable (based on exclusion of viability dye), GFP+ SMCs were sorted into buffer with 0.04% BSA, and between 2,000 to 4,000 cells from individual aortic segments were analyzed for each sample Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1