small RNA-seq of Arabidopsis thaliana: bulked MIR168a over-expressing seedlings
RNA were purified with the phenol-chloroform method, 20 g of the samples were loaded onto poly-acrylamide gel, the small RNA fraction was isolated and libraries were prepared using the Truseq Small RNA Library Preparation Kit (Illumina, San Diego, CA, USA) and the modified protocol described earlier (Czotter et al., 2018).