GSM5172888: 10.5 hpf embryos - RARaa; Danio rerio; ChIP-Seq
Embryos were dechorionated and deyolked prior to fixation with 1.85% PFA for 10 minutes.  Diagenode Bioruptor sonicator was used for chromatin shearing. Dynal Protein A magnetic beads (Diagenode) and ChIP-graded anti-myc antibody (ab9132, Abcam) were used for the immunoprecipitation, an aliquot of chromatin being taken before the immunoprecipitation step and used as negative control (input). Libraries were prepared with the NEBNext Ultra II DNA Library Prep kit