GSM4742916: AAV-PR-M-2 [bulk_RNAseq_AAV_PGR]; Mus musculus; RNA-Seq
RNA extraction was performed from sorted Pcm1(+) nuclei. Nuclei were lysed in TRIzol® Reagent (15596018, Invitrogen) and then processed with Direct-zol™ RNA Microprep kit (Zymo/ Integrated Science, R2060). Ribosomal RNA was depleted from total RNA using NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (E6310, New England Biolabs) followed by library generation using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760, New England Biolabs) to select library fragment sizes of 200-400bp.