WGS of Lepomis macrochirus: male
Dovetail staff extracted DNA from a subsample of tissue shipped to their facility following the Qiagen Genomic DNA extraction protocol for tissues and using a Qiagen Tip-100 Midi Column, and they prepared Oxford Nanopore 1D libraries from extracted DNA with slight modifications to the protocol. Modifications included using variable amounts of input DNA (3-4 g), using smaller amounts of fragmentation mix (1-2.5 L), and extending the ligation time to 20 minutes for most reactions. After preparation, long-read libraries were sequenced on an Oxford Nanopore MinION using an R9.4 flowcell, and bascalling was performed using MinKnow 1.15.1.