SequelI_4strains_SMRT
Libraries from these genomic DNAs were sequenced using the PacBio Sequel I sequencing platform. Briefly, SMRTbell libraries were constructed from genomic DNA samples following the PacBio protocol for Sequel using the kit 100-938-900. DNA qualification and quantification were performed using the Qubit fluorimeter (Invitrogen, Eugene, OR) and 2100 Bioanalyzer (Agilent Technology, Santa Clara, CA). The  library was prepared for binding following the PacBio guidelines generated by SMRT Link and run on a Sequel I machine. Barcodes were used to multiplex 4 strains: bc1018 (JZ_072), bc1022 (JB_009), bc1021(ER3718) and bc1019(ER3716).