Aedes aegypti YK_2020 WGS
Genomic DNA was extracted from a pooled sample of five <24-h-old female adults. Extracted DNA was randomly fragmented to a size of 350_bp; end polished, A tailed, and ligated with Illumina sequencing adapters using a NEBNext Ultra DNA library prep kit (New England BioLabs, Ipswich, MA, USA); and further PCR enriched with oligonucleotide primers P5 and P7. The PCR products that comprised the final libraries were purified (AMPure XP system; Beckman Coulter Life Sciences, Indianapolis, IN, USA) and subjected to quality control tests that included size distribution by an Agilent 2100 bioanalyzer (Agilent Technologies, CA, USA) and molarity measurement using real-time PCR. The libraries were then pooled and sequenced on a NovaSeq 6000 instrument (Illumina) using 2- by 150-bp chemistry by Novogene HK Company Ltd., Hong Kong.