GSM6126066: PR1; Acinetobacter baumannii; RNA-Seq
TRIzol method was used for RNA extraction. 500µg of total RNA was taken for rRNA depletion using QIAseq FastSelect −5S/16S/23S Kit (Catalog: 335925,invitrogen) according to manufacturer's protocol. This method offers selective depletion of bacterial 5S/16S/23S rRNA. In brief, the RNA sample was hybridized to the FastSelect 5S/16S/23S probe followed by the removal of this complex through QIAseq Beads included in the kit. Thus depleted RNA was eluted with NFW.