Illumina Sequencing of Hanleya hanleyi gDNA
To produce short-read data, genomic DNA was extracted from 96% ethanol-preserved samples of foot tissue using a CTAB-phenol-chloroform method following Varney et al. (2021). A sequencing library was prepared using the Illumina TruSeq DNA PCR-Free kit with dual indexing according to the manufacturers instructions. This library was sequenced by Macrogen USA on one lane of the Illumina HiSeq X instrument with 150 bp paired-end (PE) sequencing.