GSM5963762: Ova_LN_2; Mus musculus; RNA-Seq
Lymph nodes were harvested two days following treatment and cells were sorted into 4 populations : TCR-b+, CD19+, CD11c+ and CD11b+ CD11c-. Cells were recombined at a 1:1:1:1 ratio. When there were not enough CD11c+ Cells, additional CD11b+ cells were added to get to 10,000 total cells. For the CNS samples, mice were perfused with 10 mL ice cold PBS then the spinal cord was harvested. The spinal cord was then disociated with a gentlemacs dissociator and suspended in 0.33mg/mL Collagenase A for 25 minutes. Samples were then placed in 30% percoll and underlayed with 70% percoll and spun at 650G for 25 minutes no brake. Samples were then sorted for CD45+ cells for sequencing. Enriched cells were prepared for single cell libraries using the 10X Genomics Chromium Single Cell 3' Reagent Kits according to the manufacturer's instructions. scRNA-seq