16sRNA-Seq of dung the sample: Cow dung
For each sample, the 16S rRNA genes were amplified by bar code primers set 343F (5 '-GAC AGT ACG GRA GGC AGCAG-3') and 798R(5 '-GAC AGA GGG TAT CTA ATC CT-3'), 515F (Reysenbach et al., 1992) and 909 R(Bruk et al., 1998), including Illumina MiSeq cohesive subsequence.Amplifiers were purified with ultrafine POLYMERase chain reaction purification kit (MObio, USA) and the same amount of POLYMERase chain reaction products were sequenced with Illumina MISEQ TM system (Illumina, USA).