RNA seq of  human villi
The Ribo-ZeroTM Gold Kits for Illumina was used to eliminate the interference of rRNA. Using NEB Next Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ispawish, USA) following manufacturers instructions to choose different index labels to complete the RNA-seq library (Strand-Specific) construction. Then, PCR product by gel extraction to paired-end sequence on Illumina NovaSeq 6000. All data were dealt with quality control to eliminate abnormal Reads, compared with the reference genome downloaded in ENSEMBL (http://www.ensembl.org/index.html) by software HISAT2 and estimated FPKM to finish the statistics difference.