WGS_G272
Genomic DNA was extracted using Wizard Genomic DNA Purification Kit (Promega, Madison, USA). Quantity and quality were evaluated using a Qubit fluorimeter (Thermo Fisher Scientific, Waltham, MA, USA). Libraries were prepared using the TruSeq DNA PCR Free protocol (Ilumina, San Diego, USA). Then, the final quality of the libraries was assessed with Fragment Analyzer (Std. Sens. NGS Fragment Analysis kit 1- 6000 bp) and were quantified by qPCR at the Genomics and Bioinformatics Core Facility (Centre for Biomedical Research of La Rioja). Subsequent sequencing was carried out in an Illumina HiSeq 1500 (Illumina, San Diego, USA).