RNA-Seq of Canis lupus familiaris testes tissue
RNA was extracted from testes using the RecoverAll Total Nucleic Acid Isolation Kit (Thermo Fisher Scientific) according to the manufacturers instructions. Quality score were measured by Agilent Bioanalyzer on a Total RNA 6000 Nano chip to obtain RIN score for RNA integrity. Illumina libraries were generated using the TruSeq Stranded mRNA LT-Set A for the 40 samples with all having unique barcodes and library quality control was performed on Agilent Bioanalyzer. Pooled samples were run using the NextSeq High Output v2.5 75 cycle kit.