Illumina HiSeq 4000 paired end sequencing; HNF4A is required to specify glucocorticoid action in the liver - RNAseq
HNF4A is required to specify glucocorticoid action in the liver - RNAseq
Mice were treated acutely with an intraperitoneal injection of dexamethasone or vehicle, and liver tissue harvested two hours later.  RNA extraction from flash-frozen mouse liver tissue was performed using the ReliaPrep RNA Miniprep system (Promega), as per manufacturer's instructions, incorporating a DNase treatment step. Lysing Matrix D tubes (MP Biomedicals) were used to homogenise tissue.  Illumina TruSeq Stranded mRNA kit, according to the manufacturerâ€™s protocol.