GSM5738352: DA-G20 - 15µM Copper(II), rep1; Oleidesulfovibrio alaskensis; RNA-Seq
Cell pellet was collected and washed three times with anaerobic phosphate buffer saline (pH 7.2). Total RNA was extracted  using TRIzol RNA extraction method and eluted in 20 µL nuclease free water. Extracted RNA was further purified using the RNA clean and concentrator kit following manufacturer's instructions (Zymo Research, USA), and eluted in 10 µL nuclease free water. 1µg total RNA used for library preparation. The library preparation step consisted of the following steps: i) rRNA depletion by RiboCop rRNA Depletion Kit (Lexogen, New Hampshire, USA). cDNA library was prepared using Swift Rapid RNA Library kit using the manufacturers protocol (Swift Biosciences, Michigan, USA).