GSM5011630: MUSCLE_AGE60_M_GROUP50_64_CC_T300_Gt013_R1; Homo sapiens; RNA-Seq
Total RNA was prepared by lysing cell pellet (2x106) in 700ul Qiazol and extracted using Qiagen miRNeasy mini kit according to the manufacturer's recommendation (Qiagen Inc, CA, USA) Samples were prepared using Nugen Ovation v2 kits and the sequencing library was prepared using Illumina ChIP-Seq kits according to manufacturer's protocol (Illumina, San Diego, CA).