Nanopore MinION sequencing of Clostridium botulinum strain 426-HONEY isolated from contaminated honey in 1977
Strain grown anaerobically for 24h at 35C in 25 mL TPGY broth. Whole culture was pelleted and resuspended in 0.5 mL of GPB with 0.5 mL of 2x Zymo DNA/RNA Shield. DNA extracted using Zymo Quick-HMW kit and RNAseA treatment. DNA was processed (SPRI selection >1500bp), prepared with rapid barcoding sequencing kit (SQK-RBK-004) and 1D MinION sequenced using FLO-MIN106 R9.4 flowcell.