GSM6172565: pol32D BrdU 25C 30' sonication rep3 [Alb613_pol32D_BrdU_S30]; Saccharomyces cerevisiae; ChIP-Seq
For analysis of DNA replication by BrdU-IP-ssSeq, and PCNA ChIP and eSPAN experiments, chromatin was fragmented by sonication (15 seconds on and 30 seconds off for 10 cycles in a Bioruptor Pico, Diagenode). For analysis of nucleosome occupancy at nascent chromatin, H3K4me3 and H3K56ac ChIP as well as almost all eSPAN experiments except those marked by sonication, chromatin was digested with MNase to map nucleosomes (Wal and Pugh, 2012). ﻿The ssDNA libraries were prepared using the Accel-NGS 1S Plus kit (Cat no. 10096, Swift Bioscience).