DMS of NEB 5-alpha F'Iq: Single-codon substitutions in CAT-I, sequencing run 4, generation 0
A library of single-codon substitutions in CAT-I was constructed using inverse PCR. The library was transformed into NEB 5-alpha F'Iq cells. We plasmid prepped uninduced, exponentially-growing cultures of the library mixed with wildtype CAT-I and determined the allele frequencies at the start of the growth experiment as our generation 0, replica 2 sample. Data for codons 1-220 was used from these files. This was used as Replica 2 for regions 1 (codons 1-73), 2 (codons 74-146), and 3 (codons 147-220).