GSM4743704: 1-dodecyl chloride-3-methylimidazolium_rep2 - C12mimCl_R2; Daphnia magna; RNA-Seq
After exposure, active organisms were randomly collected from each replicate into RNAlater® for further storage at -80 °C. Pools of 90 individuals collected from each replicate were used for extraction. Organisms were homogenized with a disposable pestle before RNA extraction with the RNeasy kit and Qiashredder by Qiagen (Venlo, Netherlands) following the manufacturer's protocol. The library construction of cDNA molecules was carried out using the Kapa Stranded mRNA Library Preparation Kit (Kapa Biosystems) and the generated DNA fragments were sequenced in an lllumina HiSeq 4000 platform, using 150 bp paired-end sequencing reads