GSM6125704: TSCs_C1_0; Mus musculus; RNA-Seq
RNA was extracted from ESCs with a miRNeasy mini kit (217004, Qiagen), quantified with a NanoDrop spectrophotometer and its quality examined in a fragment Bioanalyzer (Aligent 2100 Bioanalyzer DNA 7500 assay). For RNA-sequencing, libraries were prepared with a TruSeq Stranded mRNA Library Preparation Kit (Illumina) followed by single-end sequencing (50 bp) on an HiSeq2500 instrument (Illumina), obtaining at least 40 million reads per sample.