GSM5963146: PBDE 9; Mus musculus; RNA-Seq
Mammary tissues were dissected, flash frozen, and stored at -80 C until being dissociated in RLT lysis buffer (supplemented with beta-mercaptoethanol) with a bead mill (Qiagen TissueLyser LT). Lysates were then processed with Qiagen Rneasy Mini Kit, utilizing qiashredder tubes for homogenization and on-column DNAse Lexogen QuantSeq 3' FWD