GSM6124649: Treated_5; Homo sapiens; RNA-Seq
Total RNA from each sample was extracted by RNeasy Micro Kit (74004；Qiagen). Then it was quantified with Quabit HS RNA Kit (Q32855; Thermo Fisher Scientific). RIN was measured by Agilent Bioanalyzer 2100 (Agilent) and only those with RIN > 7 were considered as qualified. MALBAC® KT110700796 (Xukang Medical·Yikon Genomics, Suzhou, Chian) was used for RNA reverse transcription and amplification. 1 uL of cDNA was diluted 10-fold and detected with Agilent Bioanalyzer 2100 (Agilent). cDNA size is 1000-10000bp.