GSM4743862: KYSE70_siBAF53A_1_RNAseq; Homo sapiens; RNA-Seq
72 hours after siRNA transfection, cells were lysed directly on culture plates with TRIsure reagents (Bioline), and RNA was extracted using Direct-zol RNA MiniPrep kits (Zymo Research) with in-column DNase I digestion to remove residual genomic DNA. RNA-seq libraries were generated by using NEBNext ultra II directional RNA library prep kit coupled with NEBNext multiplex oligos for Illumina (New England Biolabs) and following the manufacturer's directions.