GSM5121197: Setd1b_KO_3_RNAseq; Mus musculus; RNA-Seq
Ovaries were collection from 21-day-old females and digested with 2mg/mL collagenase (Sigma) and 0.02% trypsin solution, agitating at 37°C for 30 min.  GV oocytes were collected in KSOM medium (Sigma) and washed with PBS from females with an oocyte conditional deletion of Setd1b using Gdf9-cre (Setd1b cKO) and females carrying floxed Setd1b (Setd1b WT). Four GV oocytes for each genotype were collected for single cell RNA-seq (scRNA-seq). Oocytes deposited in 2.5 µl lysis buffer and cDNA amplified exponentially with SMARTer Ultra Low RNA Kit for 12 cycles. Library construction was performed with NEBNext Ultra DNA Lib Prep Kit.