Paired-end Illumina sequencing of Clostridium botulinum strain 426-HONEY isolated from contaminated honey in 1977
Strain grown anaerobically for 24h at 35C in 25 mL TPGY broth. Whole culture was pelleted and resuspended in 0.5 mL of GPB with 0.5 mL of 2x Zymo DNA/RNA Shield. DNA extracted using Zymo Quick-HMW kit and RNAseA treatment, and prepared with NexteraXT library prep; paired-end (2x300bp) sequencing using MiSeq and v3 chemistry.