Transcriptome raw data of Pseudobagrus ussuriensis genome sequencing
The transcriptome library used for gene annotation of the P. ussuriensis genome was constructed using tissues of liver, spleen, heart, kidney, gills, brain, pituitary, muscle, skin, gonads and intestine. Total RNA of each tissue was extracted using TRIzol Reagent Kit (Invitrogen, USA), according to the manufacturers protocol. Then 100 ng RNA from each tissue were pooled together for cDNA library construction. The NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) was used to construct the library following the manufacturer's protocol. Finally, the 150 bp paired-end sequencing was performed for the library through a high-throughput Illumina HiSeq 4000 platform at Novogene Co., Ltd., Beijing, China following the manufacturers instructions (Illumina, USA).