GSM5738184: K303R-NT; Homo sapiens; ATAC-seq
5 x 10^4 cells are processed for nuclear pellet preparation with a hypotonic buffer with digitonin. Libraries were prepared from the nuclear pellets by using Tagment DNA Enzyme kit (Illumina). Prepared DNA fragments were cleaned with DNA Clean and concentrator-5 kit (Zymo Research) and added with adaptor and index sequences by PCR with NEBNext high-fidelity 2x PCR master mix (NEB), and then purified with DNA Clean and concentrator-5 kit (Zymo Research) again. The concentration of the library was quantified using Fluorometer (Quantus) and KAPA library quantification kit (Roche), and the quality was checked by TapeStation D5000 (Agilent). The pooled libraries with unique index sequences were sequenced using MiSeq or NextSeq550 (Illumina) with pair-end 75 bp reads following the manufacturer's protocols.