GSM5563180: TZM-blB8_PSMB8-mCherry_1; Homo sapiens; RNA-Seq
Total RNA was extracted using the Extract RNA reagent (Evrogen). The concentration of RNA was measured with a Qubit Fluorometer (Invitrogen). The quality of RNA was determined with an Agilent BioAnalyzer 2100 using an RNA 600 nano kit. The RNA Integrity Number (RIN) of all RNA samples was not less than 8. PolyA enriched libraries for RNA-seq were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina according to the manufacturer's protocol. Seventy-five bp single-end sequencing was conducted on an Illumina NextSeq 500 platform.