Nanopore raw data of Pseudobagrus ussuriensis genome sequencing
The Nanopore library was constructed using an Oxford Nanopore Ligation Sequencing kit SQKLSK109 (Oxford Nanopore Technologies, UK) following the manufacturers instructions. Briefly, size-selection (10-50 kb) was initially performed using approximately 10 g of gDNA by a BluePippin pulsed-field gel apparatus (Sage Science, USA). After damage and end repairs, adenines were added at the ends of DNA segments and then adapters were ligated to finish the construction of the Nanopore library. After testing using a Qubit 3.0 fluorometer (Invitrogen, USA), the library was sequenced on R9.4 Flowcell using a PromethION sequencer (Oxford Nanopore Technologies, UK) at NextOmics Bioscience Co., Ltd (Wuhan, China). Then the ONT software Albacore v1.2.6 was used for base calling on fast5 files, and reads passed the filtration were considered as high-quality data, which were used for further analyses.