low-coverage whole genome resequencing of Lynx species:adult muscle tissue: BOBCAT_1
We prepared a separate barcoded library for each individual following the tagmentation protocol developed by Baym et al. (2015) and further optimized for high-throughput, low-cost genome generation by Therkildsen and Palumbi (2017). We modified the protocol to use one-tenth instead of one-twentieth reactions after preliminary testing revealed more consistent success across sample quality with a lower ratio. The final concentration of input DNA ranged from 1.3 - 5.3 ng/mL for each library. We combined equimolar amounts of the library into two pools for sequencing. Sequencing included one lane of 125-bp paired-end reads on an Illumina NovaSeq S4 flow cell at Novogene Corporation (Sacramento, CA, USA) and one lane of 125-bp paired-end reads on an Illumina NovaSeq S1 flow cell at The Centre for Applied Genomics (Toronto, ON, Canada).