Nanopore DNA sequencing; Tylothais aculeata; muscle
For Nanopore long reads sequencing, ~400 ng DNA were used with the the Oxford Nanopore Technologies (ONT) Rapid Library Sequencing kit (SQK-LRK001) for library construction. 30 l or 75 l of each library were loaded into a Flongle or R9.4 flow cell, respectively. The sequencing experiments were conducted with a MinION device for 24-48 hours. The nanopore FAST5 files basecalling were converted to FASTQ files by Guppy 4.4.2 (https://nanoporetech.com/community) fast mode with default setting (Oxford Nanopore Technologies, Oxford, UK).