GSM5984145: RNA-seq_Veh_rep1; Mus musculus; RNA-Seq
Neutrophils were isolated with Percoll gradient and 10 μM of PGE2 or vehicle were treated for 24 hours. After that, RNA was isolated with Trizoal reagent. 1 μg of total RNA was prepared for the library by Illumina TruSeq Standard mRNA Sample Prep Kit. RNA libraries were prepared using TruSeq Stranded mRNA Library Prep Kit