Illumina HiSeq 2000 sequencing; ChIP-seq for Tlx3 in the Mouse developing Dorsal Spinal Cord
ChIP-seq for Tlx3 in the Mouse developing Dorsal Spinal Cord
Experiments were carried out in agreement with European Community Council Directive (86/609/EEC) and the animal ethics guidelines of the i3S, and approved by the Portuguese Veterinary Ethics Committee. Dorsal spinal cord tissues were collected from E14.5 embryos and then dissected in ice-cold PBS.  Cells were fixed sequentially with di(N-succimidyl) glutarate and 1% formaldehyde in phosphate buffered saline and then lysed, sonicated and immunoprecipitated, as previously described (Castro et al., 2011; Regadas et al, 2014), with an anti-Tlx3 antibody DNA libraries were prepared from 10ng of immunoprecipitated DNA according to the standard Illumina ChIP-seq protocol.