GSM6285271: SU-DHL-4 DMSO N2; Homo sapiens; RNA-Seq
Cell pellets were resuspended in QIAzol Lysis Reagent (QIAGEN, Venlo, Netherlands) and RNA Isolation was performed with miRNeasy Mini Kit (QIAGEN, Venlo, Netherlands) according to the manufacturer's instructions Poly-A RNA was enriched from 1 µg total RNA using the NEBNext Poly (A) mRNA Magnetic Isolation Module (New England Bioabs, Ipswich, USA) and sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit (New England Bioabs, Ipswich, USA). Sequencing was conducted on an Illumina NextSeq500 (Illumina, San Diego, USA) as single reads with 75 bp length with a total of 480 million reads.