GSM6067246: Ctrl 2; Homo sapiens; RNA-Seq
After harvesting siLGALS1 and control cells, genomic DNA were removed RQ1 DNase (Promega) and we obtained total RNAs. The qualified RNAs in quality and quantity, which were measured by the absorbance at 260nm/280nm (A260/A280) using smartspec plus (BioRad), were used for following experiment by 1.5% agarose gel electrophoresis method. One μg RNA was captured by by VAHTS mRNA capture Beads (Vazyme, N401) for siLGALS1 and control samples with three biological replicates. Polyadenylated RNAs by oligo(dT) were used for RNA-seq library preparation by KAPA Stranded Kit for the Illumina® Platforms. After size selection (300-500bps), the cDNA products were processed and stored at -80°C fridge, and then used for high-throughput sequencing (RNA-seq) by Illumina Novaseq 6000 system, and finally 150 nt paired-end sequencing reads were obtained.