GSM5277256: Rosa26-WT (Control); Mus musculus; RNA-Seq
Bone marrow was isolated from 12-week-old R26-AE9a and R26-WT mice. LK cells were enriched using a lineage-depletion kit (MACS #130-090-858) and cKit enrichment kit (MACS #130-091-224). Single-cell suspensions were converted to barcoded libraries using the 10X Genomics Chromium Single Cell 3' GEM, Library & Gel bead Kit V3 and tagged using the Chromium i7 Multiplex Kit. Libraries were sequenced using Illumina Next Generation Sequencing systems (HiSeq4000). 10X Genomics single-cell RNA sequencing