GSM5012087: PD-L1: MPE cells from mice with PD-L1 treatment; Mus musculus; RNA-Seq
The mice were sacrificed on day 13 and pleural fluid was gently aspirated using a 1-ml syringe through the diaphragm, and its volume was measured with a 1 mL pipette. The pleural fluids were centrifuged at 500 g for 10 min and cell pellet was further treated with 2 mL ACK Lysing Buffer (Thermo Fisher) to delete red blood cells for 10 min at 4 °C in 15-mL conical centrifuge tube. 10 mL cold PBS was added to stop the lysis, and the cells were centrifuged at 500 g for 5 min. The cell pellets were then re-pooled with same ratio from three mice. Droplets and libraries were prepared using Chromium Single Cell 3' Reagent Kits v2 according to manufacturer's protocol (10X genomics). Generated libraries were sequenced on Illumina NOVASEQ 6000