GSM4903954: hPhf8 rep3; Xenopus tropicalis; RNA-Seq
RNA extraction was performed usingTRIzol (Ambion) and phenol/chloroform extraction. RNA clean-up and DNA digestion were performed with RNeasy Mini-Kit (Qiagen).  RNA integrity quality was analysed using an Agilent 2100 Bioanalyzer. Ribosomal RNA was removed using NEBNext rRNA Depletion Kit (Human/Mouse/Rat). Total stranded RNA sequencing libraries were prepared using NEBNext Ultra Directional RNA Library Prep Kit for Illumina according to the manufacturer's instructions