GSM6125966: noCAD_16 [EP00309]; Homo sapiens; RNA-Seq
Total RNA was isolated using KingFisher Flex Purification system and MagMax for Stabilized Blood Tube RNA Isolation kit (Thermo Fisher Scientific), following manufacturer's instructions, and precipitated through ammonium acetate/ethanol method. α and β-globin mRNAs depletion were performed from precipitated total RNA using the GLOBINclearTM Human kit (Applied Biosystems) Poly(A)+ RNA enrichment followed the Dynabeads mRNA Purification Kit protocol (Thermo Fisher Scientific). Libraries were prepared and pooled together (6 samples per sequencing run) using multiplex library RNA Barcoding reagents and Total RNA-Seq Kit v2 (Thermo Fisher Scientific).