GSM6124628: scRNAseq_WT_2; Mus musculus; RNA-Seq
scRNAseq: Single-cell suspension of V6.5 mESC was made by treatment with 0.25% trypsin followed by inactivation in complete medium. Single-cell isolation and library preparation was done using a 10x Genomics Chromium machine in 3' RNA digital gene expression mode. ChIP-seq: Cells were crosslinked in 1% formaldehyde on a rocker at room temperature for 10 minutes. Crosslinking was quenched with 250 mM glycine on a rocker at room temperature for 5 minutes. After cell lysis, chromatin was Maase-treated and sonicated using a Covaris220 evolution solicitor. ChIPseq libraries were prepared using NEB UltraII kit from 6-10 ng of DNA. Libraries were prepared by the MIT BioMicro Center.