GSM5525369: PJ292_g3_Z558Ci_S15; Homo sapiens; RNA-Seq
On the day of harvest, the cells were washed once with PBS and detached using accutase. GFP+/Drak7-  cells were collected using FACS Aria3. Cell pellets were snap frozen and kept at -80°C until RNA extraction.  RNA was extracted using the RNeasy mini kit (Qiagen) according to manufacturer's protocol. The quality and concentration of the RNA was analyzed using 2100 Bioanalyzer (RNA nano; Agilent) and Qubit (RNA HS assay kit).