GSM5115958: granulosa cells_0h_H3K4me3; Mus musculus; ChIP-Seq
Lysates were clarified from sonication/enzymatic digested nuclei and histone-DNA complexes were isolated with antibody.  The ChIP DNA and the Input DNA ends were repaired using T4 DNA polymerase, Klenow enzyme, and T4 polynucleotide kinase(PNK) (New England Biolabs), followed by treatment with Klenow exo- to add an A base to the 3' end. After ligation of the Solexa adaptor using TaKaRa ligation Mix (TaKaRa), the adaptor-ligated DNAs were amplified using Solexa PCR primers for 18 cycles, and the amplified library was isolated from an agarose gel. The samples were purified using the QIAquick MinElute kit (Qiagen) at each preparation step. The purified library was used for cluster generation and sequencing analysis using the Genome Analyzer GAIIx (Illumina).