GSM5051496: Tonsil_2b_scRNA_seq; Homo sapiens; RNA-Seq
Tonsil samples were collected from children and adults undergoing routine tonsillectomy. All participants provided written informed consent and the protocols were approved by Stanford University's Institutional Review Board. Whole tonsils were collected in saline and processed within four hours of receipt. Tissues were treated with penicillin, streptomycin, and normocin for 30 minutes on ice and heavily clotted or cauterized areas of the tissue were removed. Tonsils were then dissected into small pieces (roughly 5-8 pieces per tonsil) before mechanical dissociation through a 100um cell strainer using a syringe plunger. Mononuclear cells were isolated by ficoll density gradient centrifugation (GE Healthcare) and the buffy coats were collected. Cells were cryopreserved in 90% fetal bovine serum, 10% DMSO until use. Libraries were contructed following the 10X scATAC and scRNA protocols