Illumina sequencing of Arima Hi-C library of Cylas formicarius: adult male
Tissue from a single whole insect (icCylForm2) was crosslinked using the Arima Hi-C low input protocol, and proximity ligation was performed using the Arima Hi-C Kit. After proximity ligation, the DNA was sheared using a Diagenode Bioruptor and then size-selected to enrich for DNA fragments of 200-600bp. An Illumina library was prepared from the sheared and size-selected DNA using the Swift Accel-NGS 2S Plus DNA Library kit. The final Illumina Hi-C library was sequenced on a NovaSeq 6000 System.