GSM5277261: WT_cyclo_infarct_rep1; Mus musculus; RNA-Seq
Fresh brain tissue dissected from WT mice was immersed in RNAlater (Invitrogen, Cat. No. AM7020). Samples were assessed for quality with an Advanced Analytics Fragment Analyzer (High Sensitivity RNA Analysis Kit, Cat. No. DNF-491/User Guide DNF-491-2014AUG13) and quantity with a Qubit RNA HS Assay Kit (Cat. No. Q32852). Given satisfactory quality (RNA integrity number >8) and quantity, samples were used for library construction with the TruSeq Stranded mRNA Library Prep Kit from Illumina (Cat. No. 20020595), as well as the KAPA Dual-Indexed Adapter Kit from Roche (Cat. No. 8278555702).