RNA-seq of RAP01 KD Plasmodium falciparum NF54, 80 hpi, aTc-, rep 1
Total RNA was extracted using TRIzol, and mRNAs were purified using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB). Libraries were prepared using NEBNext Ultra Directional RNA Library Prep Kit (NEB). Final libraries were amplified by PCR with KAPA HiFi HotStart Ready Mix (KAPA Biosystems) and the PCR conditions consisted of 15 min at 37C followed by 12 cycles of [98C (30 s), 55C (10 s) and 62C (1 min 15)], finished by 5 min at 62C.