GSM5827091: LR-ROD-7; Homo sapiens; RNA-Seq
We isolated total RNA from  bone using TRI reagent (Waltham, MA, USA) and purified RNA using RNeasy kit (Qiagen, Germantown, MD, USA) and submitted 1 µg of total RNA for sequencing. Libraries were prepared according to Illumina's instructions, i.e, total RNA library for each individual sample was prepared using the TruSeq Total RNA-Seq Library Preparation Kit (Illumina, San Diego, CA), and the bar-coded cDNA libraries were sequenced for 100 bp single reads using Illumina HiSeq 4000.