Suvey raw data of Pseudobagrus ussuriensis genome sequencing
The Illumina library, which was used for polishing of the assembled Nanopore genome, was constructed using a Truseq Nano DNA HT Sample Preparation Kit (Illumina USA) according to the manufacturers recommendations. In brief, the genomic DNA was fragmented by sonication into short segments with a size of 350 bp. Then DNA segments were end polished, A-tailed, ligated with the Illumina sequencing adapters and amplified through PCR. After purification (using the AMPure XP system) of PCR amplicons, the library was analyzed for size distribution using an Agilent2100 Bioanalyzer and quantified through real-time PCR. Finally, the library was sequenced by an Illumina NovaSeq platform at NextOmics Bioscience Co., Ltd (Wuhan, China) though 150 bp paired-end sequencing.