GSM5940076: undifferentiated hiPSCs_complete_rep3; Homo sapiens; RNA-Seq
RNA was extracted using the RNeasy micro-kit (Qiagen, Germany), DNase I (Qiagen) treatment was done according to the manufacturer's instructions. Library preparation and RNA sequencing were performed by Novagene Co. ltd. Briefly, a total amount of 1 μg RNA per sample was used. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer's instructions, and index codes were added to attribute sequences to each sample. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using PE Cluster Kit cBot-HS (Illumina) according to the manufacturer's instructions. After cluster generation, the library preparations were sequenced on an Illumina platform (Novaseq 6000PE150), and paired-end reads were generated.