GSM4743559: Itgam sgScrmbl 1; Mus musculus; RNA-Seq
Each CNS was isolated per mouse. A cell suspension was generated by papain digestion, neutralization, and 70um filtration of cell extract. Cells were centrifuged, resuspended in 30% Percoll, centrifuged, and washed in PBS. Cells were sorted by flow cytometry based on a negative selection strategy for astrocytes. Libraries were created according to the manufacturer's protocol for the NEB kit (#E6420)