GSM5984047: SET_C_YD3A; Mus musculus; RNA-Seq
Tissues were harvested 3 days post wounding and digested using liberase TL. F4/80+ CD64+ cells were sorted out of the wounds and directly into Rneasy RLT lysis buffer and RNA was harvested First-strand cDNA synthesis and amplification were performed using the SMART-Seq v4 ultra-low input RNA Kit for sequencing (Takara) following the supplier's instructions. The amplified double-stranded cDNA was purified using AMPure XP beads.  For Library construction, cDNA samples were sheared using the Covaris AFA system and fragments were end-repaired, A-tailed and ligated to sequencing adaptors before PCR enrichment.  Library concentrations were quantified using a Qubit fluorometer and were diluted 2ng/µl before checking insert size on a bioanalyser. Pooled libraries were sequenced on a Novaseq6000 platform with a 150bp paired-end read length at an average depth of 50 million reads per sample.