Ilumina Sequencing of Wolbachia infected T.confusum
DNA was quantified using the Qubit dsDNA HS Assay Kit (ThermoFisher Scientific) and diluted down to 3.3 ng/L. Diluted DNAs wereused as input for Illuminas Nextera DNA Flex Sample Preparation kit,which was used by strictly following the manufacturers instructions.The libraries were quantified with the Qubit dsDNA HS Assay Kit(Thermo Fisher Scientific), and their fragment size distribution waschecked in the Agilent 2100 Bioanalyzer using the Agilent DNA 1000Kit (Agilent Technologies).All libraries were pooled in non-equimolar amounts (according to theclients instructions) and sequenced in a fraction of an Illumina NovaSeq6000 PE150 lane.