GSM6124948: MCF7, EPZ H3K79me2 ChIP-seq, rep1; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated to ~200 bp fragment using a probe sonicator. The DNA fragments were repaired, A-tailed and further ligated with Illumina adapter. The final DNA library was obtained by size selection and PCR amplification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection.