GSM5738432: DCM2 [RNA-Seq]; Homo sapiens; RNA-Seq
Heart tissue samples (20–30 mg) were cleared from pericardial fat, crushed with ceramic beads (CK14, diameter 1.4 mm, Bertin) in 350 µL of RLT lysis buffer supplemented with 3.5 µL of β-mercapto-ethanol. Total RNA was extracted from biopsies using the RNeasy Mini Kit adapted with Trizol. RNA quantity and quality were measured with a 2100 BioAnalyzer (Agilent). Ribosomal RNAs were depleted and samples were prepared for sequencing according to the Illumina TruSeq RNA Preparation Kit and subjected to pairwise sequencing (2x150bp) with an Illumina HiSeq sequencer. Sequencer results were obtained as raw fastq file format. Manufacturer protocol