GSM5738025: testis sperm rep2; Mus musculus; Bisulfite-Seq
Spermatozoa were incubated in DNA lysis buffer (100mM Tris HCL (pH 8.5), 5mM EDTA, 0.2% SDS, 200mM NaCL, 100mM DTT, 300 μg/mL proteinase K) overnight at 55 °C. Spermatic genome extraction was performed following the standard phenol: chloroform protocol. Spermatic DNA was precipitated overnight at -20 °C, and washed twice with 70% ethanol. Finally, DNA was resuspended in 30 µL DNAse-free water. libraries were prepared following Diagenode Premium RRBS kit (Diagenode, C02030032).