GSM6068489: Growth plate chondrocytes, Idh1 mutant 124-3; Mus musculus; RNA-Seq
Animals were euthanized according to IACUC protocol and hind limbs were isolated. Surrounding tissues were dissected off growth plates (GP) from distal femur and proximal tibia were washed with PBS. GP chondrocytes were digested using 2mg/ml Pronase I at 37C for 1 hour with agitation followed by two times, 3mg/ml Collagenase IV digestion at 37C. Cells were washed in PBS. Live cells were FACS sorted followed by GEM generation and RT using for 10X Genomics single cell isolation and barcoding. RNA libraries were prepared for sequencing using standard Illumina protocols