Genome  Sequencing of Rotavirus A in a Floridian Racing Pigeon
Viral RNA (vRNA) from the intestinal tissue was extracted using a RNeasy Mini Kit (Qiagen, Valencia, CA) according to the manufacturers instructions. The RNA served as a template for the generation of a cDNA sequencing library using a NEBNext Ultra Directional RNA Library Prep Kit (New England Biolabs). The library was sequenced using a v3 chemistry 600-cycle Kit on a MiSeq sequencer (Illumina). A total of 2,955,154 paired-reads with an average read length of 240bp were obtained and assembled de novo in SPAdes v3.13.0 with default parameters