DNA methylation sequencing of homo sapiens: children blood
DNA was fragmented (Covarias, USA), followed by blunt-end repair, 7'-end addition of poly-A, and barcoded adaptor connection. The products of each step were purified using the QIAquick PCR Purification Kit (Qiagen, Germany). After hybridization of the probes and DNA fragments, the captured DNA fragments were treated with the EZ DNA Methylation-Gold Kit (ZYMO Research, USA). Finally, the constructed libraries were quantified using an Agilent Bioanalyzer (Agilent, USA) and a quantitative PCR system (IBM, USA), followed by sequencing on the Hiseq X Ten platform (Illumina, USA).