Mouse C57/B6, Ncor1 and Ncor2 double knockout, animal 2
To perform whole genome sequencing, we harvested bone marrow from two Ncor1/2 DKO mice and tail clippings from a wildtype mouse. The bone marrow samples from the Ncor1/2 DKO mice underwent positive selection for CD19, using column purification with CD19 microbeads (130-121-301, Miltenyi Biotec). Column purified CD19+ B cells were subject to DNA isolation using a DNA extraction kit (11796828001, Roche). Tail sample was digested in tail lysis buffer containing 10 mM Tris pH 8.0, 100mM NaCl, 10 mM EDTA, 0.5% SDS, and proteinase K overnight in a 55Co water bath.  Nucleic acid quantification was performed using both a nanodrop (ND-ONE-W, ThermoFisher) and Quanti-iT PicoGreen dsDNA assay kit (P11496, ThermoFisher). Library preparation for whole genome sequencing was performed using the TruSeq Nano DNA kit (20015964, Illumina).  Sequencing was performed on a NovaSeq 6000 with 2x150 bp paired-end reads (Illumina).