WGS of C. raciborskii using Nanopore
A total of 800ng DNA from SO_9165_CPE was end-repaired (NEBnext ultra II end repairkit, New England Biolabs, MA, USA), cleaned up with 1x AmPure beads (BeckmannCoulter, USA). Native barcode ligation was performed with NEB blunt/ TA ligase (NewEngland Biolabs, MA, USA) using EXP-NBD104 (ONT) and cleaned with 1x AmPurebeads. Qubit quantified barcode ligated DNA sample was Adapter ligated (BAM) for 15 minutesusing NEBnext Quick Ligation Module (New England Biolabs, MA, USA). Library wascleaned up using 0.6X AmPure beads (Beckmann Coulter, USA) and finally sequencinglibrary was eluted in 15 l of elution buffer and used for sequencing.