GSM6124394: H3K27me3_M3sgCon_1; Homo sapiens; OTHER
CUT&RUN assay was performed using the commercial kit and protocol (CST, #86652). Primary antibodies (1μg per assay) for CUT&RUN are as below: anti-H3K27me3 (CST, #9733), anti-H3K27ac (Abcam #ab4729), anti-H3K37me2 (Thermo Fisher Scientific, #MA5-14867), and anti-H3K36me3 (Active motif, # 61021) After purification of DNA from CUT&RUN, the library was amplified by PCR and subjected to paired-end 50 base-pair high-throughput sequencing on an Illumina HiSeq2500 platform as previously described (Buenrostro et al., 2013) (Buenrostro et al., 2015).