GSM4904135: Perio2 scRNA-seq; Homo sapiens; RNA-Seq
Teeth (third molars) were collected immediately after extraction and preserved in sterile NaCl 0.9%, on ice for the time needed to transfer them from the clinic to the processing laboratory (< 10 minutes). The periodontium was isolated by scratching the lower two-thirds of the root of the teeth with a surgical scalpel directly into a Petri dish filled with sterile, cold Hank's Balanced Salt Solution (HBSS, Thermo Fisher Scientific, Reinach, Switzerland). The upper-third of the root was excluded to minimize contamination from the gingival epithelium. Periodontal tissues were transferred in falcon tubes filled with HBSS, centrifuged at 4°C, 300g, for 10 minutes. Tissues were digested in 10 mL Collagenase P 5 U/mL (11 213 873 001, Sigma Aldrich, Buchs, Switzerland) for 40 minutes at 37°C. After digestion, samples were disaggregated by pipetting, filtered through a 70 mm cell strainer, and resuspended in HBSS + 0.002% Bovine Serum Albumin (BSA; 0163.2, Roth AG, Arlesheim, Switzerland). library preparation was performed according to the manufacturer's indications (single cell 3' v2)