GSM6124685: 10x, sample female; Drosophila melanogaster; RNA-Seq
Nephrocytes were dissected under a fluorescence microscope from 5-7 day old Dot-Gal4>GFP.nls male and female adult flies. Ten flies at a time were dissected and samples immediately transferred into 1.5 ml EP tube with Schneider's medium on ice to avoid exposing the tissues to room temperature for a long period of time. Once 50 flies were dissected, EP tubes were sealed with parafilm and put on dry ice. Next steps involved spraying 100% ethanol to the dry ice near the tube to quickly freeze the sample and storing samples at -80oC for long-term. Libraries were generated according to 10X genomics v2 protocol.