GSM4744978: R256C_1; Saccharomyces cerevisiae; RNA-Seq
RNA was extracted using Qiagen RNeasy plus Mini kit with extra enzymatic lysis step of the yeast cell wall using zymolyase 100T (100U per 107 cells). cDNA Libraries were prepared using the Illumina TruSeq Stranded mRNA (Cat. # RS-122-2101) kit according to the manufacturer's protocol starting with 1μg total RNA. Briefly, mRNAs were purified with oligo(dT) beads to capture polyA tails, then fragmented and converted to cDNA with reverse transcriptase. The resulting cDNAs were converted to double stranded cDNAs and subjected to end-repair, A-tailing, and adapter ligation.