GSM5012125: 4 dpci ccn2a-/- cardiac ventricles- Sample 2; Danio rerio; RNA-Seq
RNA was isolated from 4 days post injured cardiac ventricles (bulbus arteriosus and atrium were dissected out from each heart) using an RNeasy micro Kit (Qiagen). To avoid genomic DNA contamination, samples were treated by on-column DNase digestion (DNase-Free DNase Set, Qiagen) during isolation. 2.2µg of total RNA was used as input for Truseq Stranded mRNA Library preparation following the low sample protocol (Illumina).