GSM5173175: Endodermal cells treated with DMSO 8 somites rep3 RNA-seq; Danio rerio; RNA-Seq
Embryos were dechorionated and deyolked prior to cell isolation by pippeting up and down several times. Singles cells were FACS sorted and GFP+ and GPF- cells were collected. Cells were pelleted and lysed. RNA was retrotranscribed using an Oligo dT primer to enriched in polyA RNA prior to library preparation using Nextera XT kit