GSM5799474: B188-p7-TR2; Homo sapiens; RNA-Seq
Qiagen AllPrep® DNA/RNA micro kit. RT and template switch with primers containing Barcode, UMI and adapter. Full length amplification of the cDNA. Fragmentation and Adapter ligation with NEB Ultra II FS kit and final amplification of the 3'-end with Illumina overhangs. Sequencing of cDNA in Read1 and sequencing of Barcode and UMI in Read2.