GSM6131535: Isolated Islets, WC, Rep3; Mus musculus; RNA-Seq
Mouse islets were dissociated using collagenase P and hand picked from residual debris. RNA was harvested using Rneasy mini plus kit (Qiagen). Poly-A selection for enrichment of mRNA was performed using poly-A magnetic beads (New England Biolabs) and cDNA library preparation was performed using the NEBNext Ultra kit (New England Biolabs).