GSM5799564: whole_EGFP_1; Homo sapiens; RNA-Seq
U2OS cells transfected with EGFP or CHERP#2 siRNA were scraped, washed with PBS and treated with cell lysis buffer [20 mM Tris-HCl (pH 8.0), 200 mM NaCl, 1 mM MgCl2, 1% NP-40] on ice for 5 min. Then, the cytoplasmic fraction was isolated by taking the supernatant after brief spin. Whole or cytoplasmic RNA was isolated by Sepasol RNA I super G (Nacalai tesque, Kyoto, Japan) according to the manufacturer's instructions. RNA-seq library was prepared using Truseq Stranded mRNA Library Prep Kit (Illumina, CA, USA) and sequenced on an NextSeq High Output (Illumina, CA, USA) following the manufacturer's protocol.