GSM5669672: Tie2+ Fibroblast like cells_LV-SCR2 [A4_SCR2]; Mus musculus; RNA-Seq
FACS sorted mice aortic Tie2+ Fibroblast like cells (TF) were cultured, transfected with LV-SCR, LV-shTEK-A or LV-shTEK-D, after puromycin selection. The RNA from these cells were purified using Macherey-Nagel Nucleospin RNA mini Kit (#740955.50, Macherey-Nagel) according to the manufacturer's instruction cDNA library was synthesized using BGI DNBSeq Stranded mRNA KIT (BGI, Hongkong), The library quality was determined using bioanalyzer and sequenced using MGISEQ-2000 System with the following specifications: 1 X 100bp, 20M paired end reads.