GSM5127803: Day 6 cells derived from HNF1B-/- hiPSCs_3 (bulk); Homo sapiens; RNA-Seq
[Bulk RNAseq Protocol]  RNA was extracted using the GenElute Mammalian Total RNA Miniprep Kit (Sigma-Aldrich) according to manufacturer's protocol. [Bulk RNAseq Protocol]  RNA-seq library construction and sequencing was carried out by the DNA pipelines core facility at the Wellcome Sanger Institute. [Bulk RNAseq Protocol]  Standard Illumina unstranded poly-A enriched libraries were prepared and sequenced using Illumina HiSeq 2500 v4 (Illumina, San Diego, CA, USA), with 75bp paired-end reads per sample and a library fragment size of 100-1000 bp. [10X scRNA-seq Protocol]  For the preparation of cells for single-cell sequencing we used the Chromium Single Cell 3′ library and gel bead kit v2 protocol. [10X scRNA-seq Protocol]  Single cell libraries were generated using the Chromium Single Cell 3′ library and gel bead kit v2 (PN #120237) from 10x Genomics. Briefly, to reach a target cell number of 2000 cells per sample, 3500 cells per sample were loaded onto a channel of the 10x chip to produce Gel Bead-in-Emulsions (GEMs). This underwent reverse transcription to barcode RNA before cleanup and cDNA amplification followed by enzymatic fragmentation and 5′adaptor and sample index attachment. Libraries were sequenced on the HiSeq4000 (Illumina). [10X scRNA-seq Protocol]  RNA-seq. Illumina-C HiSeq V4 Paired end sequencing