GSM4904540: RWPE miCLIP; Homo sapiens; OTHER
Total RNA from LNCaP and RWPE cells was isolated with TRIzol according to the manufacturer instructions. PolyA+ mRNA was isolated using the PolyATtract mRNA Isolation System from Promega. Libraries were prepared using 1 ug of polyA+ RNA as input using the Illumina protocol. miCLIP: 20 ug of mRNA was used as input for miCLIP following the previously reported protocol (Linder et al., Nat Methods 2015)