GSM4746281: OE_48h_3; Drosophila melanogaster; RNA-Seq
50 flies per replicate were frozen in liquid nitrogen and kept at -80 °C until processed. Fly heads were collected using metal sieves. Total RNA was isolated from fly heads using Trizol according to the manufacturer's instructions and treated with Turbo DNase for 30 min at 37 °C. Libraries were prepared at the iGE3 Genomics Platform in Geneva. Single reads of 50 bp were generated using an Illumina HiSeq 2500 sequencer.