GSM5625725: Abeta_2; Mus musculus; RNA-Seq
Total RNA was isolated from the cell culture using Rnasy Mini Kit (Qiagen, #74104). 1μg of total RNA was enriched for Poly-mRNA (Dynabeads mRNA purification kit, Invitrogen) and reverse transcribed to double-stranded cDNA. The cDNA was end-repaired, adenylated and ligated to Illumina indices prior to 14 cycles of PCR amplification (Ultra RNA library prep kit, NEBNext). Sequencing was performed using 20pM hybridization to  a 2x75 paired-end flow cell in HiSeq2500 (Illumina).