GSM5941847: B6WT+Mgl2DTR_eGFP_Thymic_cells - HTO; Mus musculus; RNA-Seq
An RNeasy micro kit (Qiagen) was used to isolate RNA obtained from each sample per the manufacturer's instructions. For 10X Genomics scRNA-seq, we generated libraries that measure: (1) mRNA transcript expression (RNA) and (2) tissue-specific hashtag oligos (HTO). Reverse transcription PCR and library preparation were carried out under the Chromium Single Cell 3' v3 protocol (10X Genomics) per manufacturer's recommendations and sequenced in a NovaSeq.