GSM5668661: PBS Treated; Mus musculus; RNA-Seq
Cells were run through a cell sorter, where only live CD45+ cells were acquired.  Cells were washed with 400ug/mL BSA 2x and then counted. Libraries were prepared using the 10x Genomics Chromium Next GEM Single Cell 3' GEM, Library and Gel Bead Kit v3.1, 4 rxns (PN-1000128); Next GEM Chip G Single Cell Kit, 16 rxns (PN-1000127); and i7 Multiplex Kit, 96 rxns (PN_120262).  Libraries were prepared using the manufacturers instructions following the Chromium Next GEM Single Cell 3' Reagents Kits v3.1 User Guide CG000204 Rev D. Samples were barcoded with Chromium Shared Sample Indexes. scRNA-Seq