GSM5625645: Cont3; Mus musculus; RNA-Seq
Total RNA was isolated from the livers using TRIzol (Life Technologies, Inc., Carlsbad, CA) and Direct-zol RNA MiniPrep (Zymo Research Corporation., Irvine, CA) according to the manufacturer's protocol. The Library preparation was performed using a TruSeq stranded mRNA sample prep kit (Illumina, San Diego, CA) according to the manufacturer's instructions. Sequencing was performed on an Illumina HiSeq 2500 platform in a 100 bp paired-end mode.