GSM4746184: Hypothalamus male low-protein [10-12-4-16]; Mus musculus; RNA-Seq
Brains were removed and regions of interests were sectioned, flash frozen on liquid nitrogen, and RNA was isoleted using mirVana™ miRNA Isolation Kit (AM1560). Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols.