GSM6127778: CD45_neg.1 [4_HD_BM_003_CD45_neg]; Homo sapiens; RNA-Seq
Punch biopsie, human whole skin dissociation kit (Miltenyi), human CD45+ microBeads (Miltenyi), RLT plus lysis buffer (Qiagen)+ ßME Total RNA was isolated using RNeasy plus micro kit (Qiagen). Quantity of total RNA was assessed with the Qubit 2.0 and quality was checked using a RNA 6000 Pico chip on Agilent's bioanalyzer. Barcoded mRNA seq libraries were prepared from 50 ng total RNA using a NEBNext® Poly(A) mRNA Magnetic Isolation Module and NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® according to the manual. All quality controls were done using Invitrogen's Qubit HS assay and fragment size was determined on Agilent's 2100 Bioanalyzer HS DNA assay.