GSM5526044: DC2.4 cells, untreated 2; Mus musculus; RNA-Seq
Rneasy mini kit as directed by manufacturer (Qiagen) Libraries for RNA-Seq were prepared with KAPA Stranded RNA-Seq Kit. The workflow consists of mRNA enrichment, cDNA generation, and end repair to generate blunt ends, A-tailing, adaptor ligation and PCR amplification. Different adaptors were used for multiplexing samples in one lane. Illumina Hiseq3000 for sequencing, Illumina Bcl2fastq2 v 2.17 program for demultiplexing