GSM5668922: hCMEC1_RNA; Homo sapiens; RNA-Seq
RNA was extracted using Trizol reagent. ATAC libraries were processed as previously described (Corces et al., 2017). The quality of purified DNA libraries was assessed using the Agilent High Sensitivity DNA kit (Agilent Technologies). Paired end, 2 x 75 bp sequencing was performed on an Illumina Nextseq 500 instrument. RNA from all samples was thawed and following confirmation of integrity and concentration using a Bioanalyzer, 100 ng was used for low-input library preparation using the NEBNext Ultra II RNA Library Prep kit for Illumina. The libraries were then quantified and sequenced using an Illumina NovaSeq 6000 at a depth of 20 million reads per sample.