GSM4784017: HCT116 WT Hi-C Replicate 1; Homo sapiens; Hi-C
Hi-C data was generated using the Arima-HiC kit (Cat. #A510008), according to manufacturer's protocols. Cells were cross-linked with 2% formaldehyde and 1-5 μg of DNA was used per Hi-C reaction. The Arima kit uses two restriction enzymes: ^GATC (DpnII), G ^ANTC (N can be either of the 4 genomic bases) (HinfI), which after ligation of DNA ends generates 4 possible ligation junctions in the chimeric reads: GATC-GATC, GANT-GATC, GANT-ANTC, GATC-ANTC. Hi-C libraries were prepared using the KAPA Hyper Library Prep Kit with a modified protocol provided by Arima.