GSM5012290: M1_12hrs_scRNAseq; Homo sapiens; RNA-Seq
scRNA-seq protocol; SureCell WTA 3_ Library Prep Kit for the ddSEQ System (20014279, Illumina). Approximately 15,000 HL-60, macrophages and activated macrophages were collected for scRNA-seq. Briefly, cellular viability was assessed and cells were subsequently washed with PBS prior to library preparation. scRNA-seq protocol was performed as previously described Illumina 2016). Cells were lifted using trypsin. Total RNA was extracted by using the RNeasy kit (QIAGEN).