GSM5828863: mRNA-Seq P14 het testicular replicate 1; Mus musculus; RNA-Seq
Total RNA of testes was extracted using Trizol reagent (15596026; Invitrogen). Poly(A) mRNA was isolated from total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB,E7490L). Libraries for mRNA abundance examination was prepared by using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB). Libraries for ribosome footprints examination was prepared by linker ligation, reverse transcription, circularization as described previously (Ingolia et al., 2012).