GSM5984073: ESC budesonide_rep3 [GM9]; Mus musculus; RNA-Seq
Total RNA was extracted using the RNAeasy kit. Lexogen's QuantSeq kit provides a library preparation protocol designed to generate Illumina compatible libraries from polyadenylated RNA. The QuantSeq protocol generates only one fragment per transcript, resulting in extremely accurate gene expression values, and the sequences obtained are close to the 3' end of the transcripts.