GSM4745006: Male6_cpi_rep2; Mus musculus; RNA-Seq
Total RNA was isolated from male and female GBM cells that were treated with DMSO (0.05%) or JQ1 (500 nM for 24 hours) or CPI0610 (1µM for 24 hours) using the RNeasy Mini Kit from QIAGEN (Hilden, Germany), following the kit protocol. PolyA Selection was performed to create RNA Seq libraries. Cell mRNA was extracted from total RNA using a Dynal mRNA Direct kit. The quantity of RNA was measured using a spectrophotometer (NanoDrop 2000c; Thermo Scientific). Samples with an RNA concentration A260/A280 ≥1.8 ng/μl, and purity A230/A260 ≥ 2.0 ng/μl were selected. The Agilent 2100 Bioanalyzer was used to determine the RNA integrity number. The degradation level was identified using the RNA 6000 Nano LabChip kit (Agilent). Samples with RNA integrity number > 9.8 were further processed using TruSeq mRNA Library Preparation Kit (Illumina) and then sequenced on a HiSeq 25000 (Illumina).