GSM5668422: EPCR-Low 2-2; Mus musculus; ATAC-seq
According to the manufacturer's instructions, sorted HSCs were resuspended in lysis buffer and incubated on ice for 5 minutes. Lysed cells were washed, strained, and nuclei were resuspended. Isolated nuclei were then used as input following the 10X Genomics Chromium Next GEM Single Cell ATAC Reagent Kits v1.1 manual (10X Genomics, Pleasanton, CA). With a target of 5,000 nuclei recovery, samples were added to the tagmentation reaction, loaded into the Chromium Controller for nuclei barcoding, and prepared for library construction following the manufacturer's protocol