GSM5128971: iFTSEC283 p53R175H treated with vehicle control (Replicate 2); Homo sapiens; RNA-Seq
Cells were harvested from 100mm plate at 80% confluency and processed for total RNA extraction using the RNeasy Plus Mini Kit following the manufacturer's protocol Libraries were prepared using the Nugen Universal RNA Seq Kit (NuGEN Technologies, San Carlos, CA) followed by sequencing using Illumina NextSeq500 instrument