GSM5012301: M2M1_repolarized_snATACseq; Homo sapiens; RNA-Seq
snATAC-seq protocol; SureCell ATAC-Seq Library Prep Kit (17004620, Bio-Rad) for the ddSEQ system (20014279, Illumina). Approximately 60,000 HL-60, macrophages and activated macrophages nuclei were collected for snATAC-seq. Briefly, cellular viability was assessed and cells were subsequently washed with PBS and nuclei was extracted prior to library preparation. snATAC-seq protocol was performed as previously described (Lareau et al., 2019; Corces, et al., 2017) Cells were lifted using trypsin. Total RNA was extracted by using the RNeasy kit (QIAGEN).