GSM4745302: scVDJ-CMVCTLs #4 TCR; Homo sapiens; OTHER
An input volume of cells was adjusted as 1,000 cells were estimated to be recovered. Individual cell beads in emulsion with single cells (GEMs) were generated with 10x Genomics Chromium Single Cell A Chip (10x GENOMICS) and Chromium Single Cell 5' Library & Gel Bead Kit (10x GENOMICS) on Chromium Controller (10x GENOMICS) followed by RT within each GEMs. Simultaneously, human TCR regions were enriched with specific primers from the cDNA above using Chromium Single Cell V(D)J Enrichment Kit, Human T Cell (10x GENOMICS). sc-VDJ-RNA-seq