GSM6131546: GFP-transduced [MXD4WT] Bio rep.1; Homo sapiens; RNA-Seq
Total RNA from these 6 samples was extracted with RNeasy Mini and Micro kits (Qiagen) followed by quality control using capillary electrophoresis (RNA 6000 Nano chips, Agilent). Libraries were prepared according to Illumina's instructions with theTruSeq RNA Library Prep Kit v2. Briefly, total RNA (1µg) was purified and fragmented before first strand and second strand cDNA synthesis. Then ends are repaired and 3'ends adenylated before adapters ligation and PCR amplification. Libraries were then validated, normalized, and sequenced on Illumina HiSeq 1000 as short-insert paired-end libraries with read lengths 100 bp.