GSM5765457: Jak1KO_aPD1_1; Mus musculus; RNA-Seq
On 14 days post-inoculation, approximately 50 mg of tumours were dissected and rapidly frozen with dry ice and IPA. Frozen tumour samples were homogenized in MACS M Tubes (Miltenyi) using the MACS Dissociator (Miltenyi). Total RNA was isolated from the homogenate using the RNeasy Plus Mini Kit (Qiagen). Library preparation with TruSeq RNA Library Prep Kit (Illumina) and mRNA NGS sequencing (40x106 paired end read) were performed by Admera Health (South Plainfield, NJ).