GSM5918486: 4_1; Glycyrrhiza uralensis; RNA-Seq
Total RNA was extracted from the tissue using Plant RNA Purification Reagent for plant tissue according the manufacturer's instructions (Invitrogen) and genomic DNA was removed using DNase I (TaKara). Then RNA quality was determined by 2100 Bioanalyser (Agilent) and quantified using the ND-2000 (NanoDrop Technologies). Only high-quality RNA sample(OD260/280=1.8~2.2, OD260/230≥2.0, RIN≥8.0, 28S:18S≥1.0, >1μg) was used to construct sequencing library. RNA libraries were prepared for sequencing using standard Illumina protocols