GSM5765352: C96_Bj3_Trap; Glycine max; RNA-Seq
TRAP was modified based on the method by (Zanetti et al., 2005) as described previously (Castro‐Guerrero et al., 2016). The optimized protocol required 10 g of root material per sample when using cortex-layer specific HisFLAG-GmRPL18 expressing hairy root. This was sufficient to obtain at least 500 ng of mRNA for library preparation and subsequent RNA sequencing. In this study, three biological replicates were prepared for each time point (72 and 96hr) for each treatment (mock- and B. japonicum-inoculated). RNA libraries were prepared for sequencing using standard Illumina protocols