GSM5667941: TM_GL_C2; Mus musculus; RNA-Seq
Single cells, reagents, and a single Gel Bead containing barcoded oligonucleotides are encapsulated into nanoliter-sized GEMs (Gel Bead-in-Emulsion) using the GemCode platform. Lysis and barcoded reverse transcription of RNAs from single cells is performed. Enzyme fragmentation, A tailing, adaptor ligation and PCR are performed to obtain final libraries containing P5 and P7 primers used in Illumina bridge amplification. Size distribution and molarity of resulting cDNA libraries were assessed via Bioanalyzer High Sensitivity DNA Assay (Agilent Technologies, USA)