GSM5862100: RIPK1 KO CD45+ rep2; Mus musculus; RNA-Seq
Mice were euthanized and tumors were harvested and dissociated using type IV collagenase solution supplemented with DNAseI. Cells were stained with CD45.2 antibody and live dead aqua and sorted for either CD45+ or CD45- live cell populations. Single cell suspensions were loaded into the 10x Genomics Chromium Controller to generate single cell emulsions. Libraries were prepared from single cell emulsions using the Chromium Single Cell 3' Library & Gel Bead Kit v2 (10x Genomics) following the manufacturer's protocol.