GSM5987854: SEPSIS patient_P28; Homo sapiens; RNA-Seq
Total RNA isolation of leukocytes from 9 COVID-19 patients on day1 and 18 sepsis patients in the Osaka Covid-19 Cohort was performed using the PAXgene™ Blood RNA System (BD Bioscience, San Jose, CA, USA). The collection tubes were stored after blood collection until further analysis at -30°C. Full-length cDNA was generated using a SMART-Seq HT Kit (Takara Bio, Mountain View, CA) according to the manufacturer's instructions. An Illumina library was prepared using a Nextera DNA Library Preparation Kit (Illumina, San Diego, CA) according to SMARTer kit instructions. DNA libraries were converted to libraries for DNBSEQ using MGIEasy Universal Library Conversion Kit (App-A).