GSM5525850: KO-3; Mus musculus; RNA-Seq
Colon tissue were removed, flash frozen on dry ice, and total RNA was extracted using RNeasy mini kit (Qiagen, Germany) and checked for a RIN number to inspect RNA integrity by an Agilent Bioanalyzer 2100 (Agilent technologies, USA). RNA libraries were prepared for sequencing using standard Illumina protocols