GSM5525844: SW480_sgITPR3_3; Homo sapiens; RNA-Seq
Livers were chopped, digested with collagenase IV, and red blood cells lysed in ACK buffer. Tumor cells were then isolated by density gradient followed by a mouse cell depletion kit (Miltenyi Biotec). RNA was extracted using the Total RNA purification kit (Norgen Biotek). Lexogen 3' FWD kit