GSM5115847: CJM-input_L2; Homo sapiens; RNA-Seq
Total RNA isolation from pterygium and conjunctival was performed using the TRIzol reagent and a Qiagen RNeasy kit, following the manufacturer's instructions. RNA was reversed using random hexamer priming (Applied Biosystems). Ribosomal RNA was removed from the products, and the first strand of cDNA was synthesized by SMART. PCR amplification and library purification were used to obtain an ultramicro-RNA methylated m6A library. Bioptic Qsep100 Analyzer was used to conduct quality control over the library.