GSM5918592: Wild type control; Mus musculus; RNA-Seq
Single cell suspensions of control and Zscan2-KO testis cells were created from n=3 mice for each. The spermatogonia population was isolated using THY1+ MACS beads, and a small number of cells from the total testis cell suspension were 'spiked' back in with these spermatogonia,creating a testis cell population enriched for spermatogonia. An equal number of cells from each of the 3 biological replicates was combined in a single 'wild type' or 'Zscan2-KO' tube. Live cells were loaded into a Chromium Controller (10X Genomics, Inc.). Single-cell cDNA libraries were generated using v2 chemistry according to the manufacturer's protocol (10X Genomics, Inc.). scRNA-seq