GSM5861614: tagcounts_WGREST_SKNSH; Homo sapiens; OTHER
Total RNA was purified by using Maxi RNeasy (QIAGEN) from homogenized cells. Then GFP mRNA was isolated by using biotinilated primers and magnetic beads followd by cDNA synthesis. Libraries were initially amplified from cDNA or plasmid, and then secondarily amplified and added P5 and P7 tag sequences.