GSM5738987: Uninfected Vero 1; Chlorocebus sabaeus; RNA-Seq
Media was aspirated and replaced by addition of 200uL cellstripper for 15 minutes, followed by scraping to resuspend the cells. The suspension was spun at 17000 RPM for 5 minutes, aspirated, and the pellet resuspended in 100uL RNALater. Extraction was performed using RNeasy extraction kits. Extracted RNA was ribo-depleted using the Ribo-Zero Gold Epidemiology rRNA removal kit, followed by NEBNext Ultra RNA library prep.