GSM5321309: HeLa-1-0min; Homo sapiens; ChIP-Seq
Cells were harvested and wash once with cold 1x PBS buffer, and then resuspended in 1 mL cold lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% NP-40, 1mM PMSF). This cell lysis reaction was incubated on ice for 10 min. DNA libraries were prepared for sequencing using TruePrepTM DNA Library Prep Kit V2 for Illumina（）.