GSM5861418: RNAseq_GM10253A_1; Homo sapiens; Cricetulus griseus; RNA-Seq
Total RNA was extracted from cells using RNeasy mini kit (Qiagen) with on-column DNase I digestion (RNAse-Free DNase Set, Qiagen). Ribosomal RNA was depleted using Illumina® Ribo-Zero Plus rRNA Depletion Kit (Illumina, 20040526) following the manufacturer's instructions. Libraries for RNA-seq were prepared and indexed using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7645L) and NEBNext Singleplex Oligos for Illumina (NEB #E7335, E7500) following the manufacturer's instructions. Single-end RNA-seq of 50-bp read length was performed on Illumina Hi-Seq 2000.