GSM4904716: sd1-PASCs-1; Rattus norvegicus; RNA-Seq
P3 SASCs, PASCs, and EASCs were cultured in 12 well plates with six replicates in each group. Each well was ground with 1 mL Trizol solution, placed at room temperature for 5 minutes, and then aspirated. RNA was separated using chloroform (mixture: chloroform = 5:1) and precipitated by isopropanol and absolute ethanol. Finally, RNA was precipitated with DEPC water, and then stored at -80°C for subsequent transcriptome sequencing (Guangzhou Ruibo company，guangzhou, China). RNA libraries were prepared for sequencing using standard Illumina protocols