GSM5625700: 0.1% BIT 5H 8_30_17_F; Pseudomonas aeruginosa; RNA-Seq
One millilitre samples for RNA-sequencing were collected immediately before exposure, 5 and 24 hours after exposure from the untreated and 10X MIC conditions. Samples were mixed with 2 mL of RNAprotect (Qiagen) to stabilize RNA and frozen at -80 C. RNA libraries were prepared for sequencing using standard Illumina protocols. Ribosomal RNA was depleted using the Ribo-Zero rRNA removal kit (Illumina). Libraries were sequenced on an Illumina HiSeq4000, 2x150bp configuration