GSM5862048: LSK_young [scRNA-seq]; Mus musculus; RNA-Seq
HSPCs from transplanted Ly5.1 mice were sorted by flow cytometry. bone marrow (BM) cells were stained with the following antibodies for sorting. BM cells were incubated with a lineage mix of biotinylated antibodies together streptavidin eF450, anti-cKit-APC and anti-Sca-1-PE/Cy7. After sorting in PBS 0,04% BSA, cells were washed, counted with an automated cell counter (EVE, NanoEnTek) and adjusted to a concentration of 1000 cells/µL. Libraries were constructed using the Chromium Single Cell 3′ Library Construction Kit v3 The mRNA-derived cDNAs were processed following the standard 10X Genomics protocol for cDNA library generation.  The scRNA-Seq libraries were sequenced an Illumina NextSeq 500 platform