GSM5173269: tx-j+PCA_H3K9ac_2; Mus musculus; ChIP-Seq
After 24 weeks, Liver were removed, flash frozen in liquid nitrogne. Liver tissue lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Sequencing libraries were generated  by using KAPA Hyper Prep Kit (Kapa Biosystems, Wilmington MA, catalog. # KK8502) which included end repair, A-tailing, and adapter ligation with the KAPA Unique Dual-Indexed Adapter Kit (Kapa Biosystems, Wilmington, MA, catalog # KK8727) according to the manufacturer's instructions.