GSM4745437: AEG-1-WT hepatocytes, CP775146-treated rep1; Mus musculus; RNA-Seq
Total RNA was extracted using the QIAGEN miRNAeasy Mini Kit (QIAGEN, Valencia, CA). cDNA preparation was done using ABI cDNA synthesis kit (Applied Biosystems, Foster City, CA). Real-time polymerase chain reaction (RT-PCR) was performed using an ABI ViiA7 fast real-time PCR system and Taqman gene expression assays according to the manufacturer's protocol (Applied Biosystems, Foster City, CA) RNA-Seq library was prepared using Illumina TruSeq RNA sample preparation kit and subject to two rounds of sequencing on Illumina HiSeq3000 platform RNA-Seq libraries were pooled together to aim about 25-40M read passed filtered reads per sample.