GSM5592068: HMLE_sRNA_replicate_1; Homo sapiens; miRNA-Seq
RNA was extracted using Trizol (Ambion, #15596018) according to the manufacturer's protocol The quantity and quality of the RNA samples were checked using nanodrop Qubit HS RNA (Invitrogen, # Q32852) and Bioanalyzer small RNA assay (Agilent, #5067-1548). Libraries were generated using 1 ug of the total RNA via QIAseq miRNA kit (Qiagen, # 331502) with 14 cycles of amplification. Amplified barcoded libraries were then size selected using auto gel-purification Pippin prep 3% agarose (SAGE science) which targets ranges from 100-250bp. Libraries (size between 180-190bp) were then confirmed by Qubit HS DNA and Bioanalyzer HS DNA assay for size and concentration.