GSM5591846: AB87; Mus musculus; RNA-Seq
Embryos were dissociated with Accutase (Sigma) into singlet cells immediately after collection, the in vitro differentiated cells were dissociated by 0.1% trypsin. Samples were flow sorted using AriaIII (BD Biosciences, BD Diva Software) cell sorter. 3' end mRNA libraries were prepared for sequencing using MARS-seq (Jaitin et al, Science 2014)