GSM5668333: CNO 9; Homo sapiens; RNA-Seq
RNA was extracted from the blood samples using the PAXgene Blood RNA Kit library preparation and RNA-Seq were conducted by Novogen Inc.. Globin mRNA was removed from the total RNA using the GlobinClear Kit. mRNA was enriched using oligo(dT) beads, and rRNA was removed using the Ribo-Zero kit. First, the mRNA was fragmented randomly by adding fragmentation buffer, and cDNA was synthesized using an mRNA template and random hexamer primers, after which a custom second-strand synthesis buffer (Illumina), dNTPs, RNase H, and DNA polymerase I were added to initiate the second-strand synthesis. Second, after a series of terminal repairs, A ligation, and sequencing adaptor ligation, the double-stranded cDNA library was completed through size selection and PCR enrichment.