GSM5669488: Mutant stimulated replicate 1; Homo sapiens; RNA-Seq
Cells were washed out of PBS prior to extraction. Using inDrops, cells were encapsulated into 4-nL droplets on ice and lysed using a final concentration of 0.4% NP-40. Single-cell lysates were subject to reverse transcription at 50°C without purification of RNA (as previously described in Klein et al., Cell 2015). Libraries were prepared as in Zilionis et al., Nat. Protoc. 2017, with modifications summarized in Supplementary Table 7 of Tusi et al., Nature 2018.