GSM6128164: BKO_m6A MeRIP-Seq; Mus; RIP-Seq
Total RNAs were extracted using TriPure Isolation Reagent (Roche, Mannheim, Germany) from iBAT of Wtapflox/flox and BKO mice . Poly(A)+ RNA was purified using Dynabeads™ mRNA Purification Kit (Invitrogen) following the manufacturer's instructions. Fragmented poly(A)+ RNA was incubated with m6A antibody (Synaptic System, 202003) for immunoprecipitation. Then, immunoprecipitated mRNAs or Input was used for library construction with NEBNext ultra RNA library prepare kit  for Illumina (New England Biolabs). The library preparations were sequenced on an Illumina Novaseq 6000 platform with a paired-end read length of 150 bp according to the standard protocols.