GSM6128380: PKD1RC minus input Rep 2; Mus musculus; ChIP-Seq
Cells were fixed with 37% formaldehyde for 20 minutes. After quenching and cell lysis, chromatin was fragmented with micrococcal nuclease Libraries from 5–10 ng ChIP DNA are prepared using KAPA HTP Library Preparation Kit. The ChIP DNA is quantified on the Qubit® 2.0 Fluorometer from Invitrogen Samples are end repaired, 3' ends adenylated and barcoded with mµltiplex adapters. PCR amplified libraries are purified with Ampure XP beads, then checked on the Agilent 2100 Bioanalyzer to ensure the library is the expected size. Samples are quantified by Qubit before being normalized and pooled, then run on the Illumina HiSeq 2500 using 50SR SBS v3 reagents.