GSM5799261: Shank3_veh_2_PFC; Mus musculus; RNA-Seq
RNA extraction from PFC samples of biological duplicates in each group used the RNeasy Mini kit (Qiagen), coupled to an RNase-free DNase step (Qiagen). The strand-specific RNA sequencing libraries were generated from 1 μg purified RNA. The RNA sequencing libraries were constructed by TruSeq stranded total RNA plus Ribo-zero kits (Illumina). Sequencing was carried out with the HiSeq 2500 platform (Illumina) at the Genomics and Bioinformatics Core of State University of New York at Buffalo.