GSM5012226: M1_3hrs_Rep1_bRNAseq; Homo sapiens; RNA-Seq
Smart-seq2 protocol (Picelli et al., 2013); Nextera DNA Flex Library Prep Kit (20018704, Illumina) Approximately ~1 million HL-60, macrophages and activated macrophages were collected for bulk RNA-seq. Briefly, cellular viability was assessed and cells were subsequently washed with PBS prior to library preparation. Bulk RNA-seq protocol was performed as previously described (Mortazavi et al., 2008). Cells were lifted using trypsin. Total RNA was extracted by using the RNeasy kit (QIAGEN).