GSM6127792: KPC_6mo; Mus musculus; RNA-Seq
Samples were mechanically and enzymatically digested (Collagenase V; 1 mg/mL in RPMI) under constant agitation. Single cell suspensions were obtained by filtering consecutively through 500 µm, 100 µm, and 40 µm cell strainers. Dead cells were removed using MACS Dead Cell Removal Kit (Miltenyi Biotec). Single-cell cDNA libraries were prepared and sequenced using the 10x Genomics platform at the University of Michigan Advanced Genomics Core.