GSM5012469: Seeds sample Rc rep 3; Hordeum vulgare; miRNA-Seq
The embryo and the scutellum from dry seeds were dissected and were crushed into fine powder in a mortar under liquid nitrogen. miRNA was isolated using a Micro RNA kit (A&A Biotechnology). The miRNA was quantitatively evaluated using Nanodrop 1000 spectrofotometer (Thermo Fisher Scientific) and agarose electrophoresis. To confirm the results and evaluate miRNA integrity 2100 Bioanalyzer with Small RNA Kit (Agilent) was used.  NebNext Multiplex Small RNA Library Prep Set for Illumina (New England, BioLabs)