GSM5592258: SCP32B; Homo sapiens; RNA-Seq
RNA lysates were prepared using the PureLinkTM RNA Mini Kit (Life Technologies, Cat. No. 12183025). The RNA concentration was measured using the Epoch microplate spectrophotometer (BioTek). Prior to library generation for RNA-sequencing, the quality of the RNA was determined using a 2200 TapeStation and RNA screen tapes (Agilent Technologies). The mRNA libraries were generated by the Bauer core facility at Harvard University using the KAPA Stranded RNA Hyperprep kit (Roche) and included polyA enrichment. The quality of these libraries was assessed by a 2200 TapeStation analyzer using D1000 screen tapes (Agilent Technologies), and q-PCR.