GSM5940952: NONO eCLIP IP - SKBG-1(S) treated - rep1; Homo sapiens; RIP-Seq
22rv1 replicates were treated with 400mJ of UV using the CL-1000 UV Crosslinker, harvested in ice cold PBS and pelleted by centrifugation. Cell pellets were flash frozen in liquid nitrogen and stored in -80 degrees until ready to IP with NONO antibody-bound Dynabeads. Subsequent protocol was followed as detailed in Van Nostrand et al, Methods Mol Biol 2017. Sequencing was performed on Illumina Novaseq 6000 single-end reads and data processed through Dr. Yeo's seCLIP pipeline. Libraries were constructed as per the (s)eCLIP protocol using Illumina 50x forward and 70x reverse primers.