GSM5668510: RW01001: CAL-33_vec_rep1; Homo sapiens; RNA-Seq
RNA was extracted from cell lines using TRIzol®. RNA quality was checked on a Bioanalyzer 2100 (Agilent Technologies). RNA-seq library preparation was performed using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD (Lexogen) according to the manufacturer's instructions. In order to avoid distortions of gene expression values due to over-cycling, the optimal numbers  of PCR cycles for library preparation were determined by qPCR according to the library preparation manual. Library quality was checked on a Bioanalyzer 2100 using a High Sensitivity DNA Kit for correct insert size and dsDNA was quantified using Qubit dsDNA HS Assay (Thermo Fisher Scientific).