GSM5942942: 220228_L2 ΔCTL0480 infected rep3; Homo sapiens; RNA-Seq
Primary cells were harvested for Total RNA at 24 hpi using TRIzol (Thermo Fisher Scientific 15596026) and the DNA-free DNA removal kit (Thermo Fisher Scientific AM1906), according to manufacturers' protocols, and were subsequently assayed for fragmentation using an Agilent Bioanalyzer 2100. cDNA library preparation of intact samples was performed using the NuGEN Universal mRNA-Seq Library Preparation kit, according to the manufacturer's protocol.