GSM5984960: T+R_P8_rep2; Ruegeria pomeroyi DSS-3; Thalassospira sp. HF15; RNA-Seq
RNA was extracted from filters using the ZymoBIOMICS RNA Miniprep Kit (Zymo Research), treated with Turbo DNase (Invitrogen, Waltham, MA, USA), and cleaned using RNA Clean & Concentrator (Zymo Research). Ribosomal rRNA was removed using the NEBNext® rRNA Depletion Kit (NEB, Ipswich, MA, USA). Stranded RNA-seq libraries were prepared and sequenced using an Illumina NextSeq 500 SE75 High Output flow cell at the Georgia Genomics and Bioinformatics Core (University of Georgia). Mono-culture samples of three bacterial species were pooled together for RNA-seq library prep and sequencing run.