GSM6130667: s0039_mouse18_HBRX3078_Tumor_1cells; Homo sapiens; RNA-Seq
Primary tumors and lung metastases were mechanically and enzymatically digested with a collagenase/hyaluronidase solution (Stem Cell Technologies, 07912). Human cancer cells were recovered by FACS sorting, based CD298 or GFP expression. Single cell isolation was performed on the Fluidigm C1 platform according to  manufacturer protocol. mRNA was isolated with the Takara SMART-Seq Ultra Low RNA kit (Takara Bio; 634833) . Libraries for Illumina sequencing were prepared with the Nextera XT DNA Library Preparation Kit (Illumina; FC-131-1096).