GSM4746310: w1118 ChIP-seq rep1; Drosophila melanogaster; ChIP-Seq
~8000 14-day-old flies per sample were collected 2 h before lights-on (ZT22), frozen in liquid nitrogen and maintained at -80 °C until processed. Heads were collected using metal sieves, and then, 1 ml of heads was ground in liquid nitrogen and homogenized 30 times in NE Buffer with 1% PFA. Lysates were clarified from sonicated nuclei and Fer2-DNA complexes were isolated with antibody. Libraries were prepared with 0,5 to 0,7 ng of ChIP-enriched DNA as starting material and processed using the Illumina TruSeq ChIP kit according to the manufacturer's specifications, at the iGE3 Genomics Platform in Geneva. The libraries were validated on a Tapestation 2200 (Agilent) and a Qubit fluorometer (Invitrogen - Thermo Fisher Scientific). Single reads of 50 bp were generated using the TruSeq SBS HS v3 chemistry on an Illumina HiSeq 2500 sequencer.