GSM6128362: En2 wild type, biological replicate #2; Arabidopsis thaliana; RNA-Seq
Total RNA was extracted from 7 days-old MS media grown seedlings using TRI Reagent® (SIGMA according to the manufacturer's protocol) The libraries were prepared for 100bp paired-end sequencing using TruSeq RNA Sample Preparation Kit (Illumina, CA, USA). Quality of these cDNA libraries was evaluated with the Agilent 2100 BioAnalyzer (Agilent, CA, USA). Following cluster amplification of denatured templates, sequencing was progressed as paired-end (2×100bp) using Illumina HiSeq2500 (Illumina, CA, USA).