GSM5124290: 677_GFPneg; Mus musculus; RNA-Seq
GFP+ GBM was macrodissected, papain-based and mechanically dissociated. A debris removal step was performed, and blood cells were removed using a blood lysis buffer. Cells were labeled with eBiosciences Fixable Viability Dye Fluor 450 and sorted using the S3e cell sorter (Biorad). GFP+ and GFP- live cells were collected, centrifuged and resuspended in a final volume of HBSS-0,1%BSA to get a 3400 cells/µL solution. Libraries were prepared according to Illumina's instructions accompanying the SureCell WTA 3′ Library Prep Kit. The cartridge was loaded with three GFP+ cell suspensions (GFP-1,2,3) and one GFP- cell suspension (GFPneg). Libraries were sequenced on the ddSEQ Single-Cell Isolator (Biorad) following the manufacturer's protocols. paired-end. R1 contains the metadata, R2 mRNA