GSM5799556: Pulmonary LAM Tumor cells  (1uM Rapamycin) - rep1; Homo sapiens; RNA-Seq
Adherent pulmonary LAM tumor cells in culture were washed twice with phosphate buffered saline (PBS) before RNA isolation using the Rneasy mini kit (Qiagen) according to the manufacturer's instructions. RNA quality was electrophoretically assessed using a Bioanalyzer and only samples with RNA Integrity values greater than 8 were used for RNA sequencing. Library preparation was performed using illumina TruSeq RNA prep kit according to the manufacturer's protocol.