GSM4745529: HTT_HEPG2_RNA_REP2; Homo sapiens; OTHER
For the HTT locus experiments, RNA was extracted using the Total RNA Purification Kit (Norgen) and mRNA isolated using DynaBeads poly-A Selection Kit (Invitrogen). RNA samples were treated with DynaBeads poly-A Selection Kit (Invitrogen) to remove DNA contamination. DNA was purified using the DNEasy Kit (Qiagen).  For the SORT1 locus experiments, both DNA and RNA were isolated using the Qiagen Allprep kit (Qiagen). From total RNA, mRNA was purified using using the mRNA Mini Kit (Oligotex). RNA samples were treated with DynaBeads poly-A Selection Kit (Invitrogen) to remove DNA contamination. RNA samples were subject to reverse transcription using a gene specific primer targeting reporter RNA.  Both harvested transfected DNA and cDNA were amplified via PCR using reporter specific primers.  Sequencing adaptors were added by further PCR in separate round.