Illumina HiSeq 2000 paired end sequencing; Transcriptional analysis of polyamine responsive genes
Plant tissue was harvested at indicated time points and frozen immediately in liquid nitrogen. Samples were stored at -80 ºC until further processing. Total RNA was extracted using TriZol reagent (ThermoFisher) and further purified using RNAeasy columns (Qiagen) Libraries were prepared using the Illumina TruSeq Sample Preparation Kit according to manufacturer's instructions.