GSM5861620: LT-HSC young rep1; Mus musculus; ATAC-seq
HSPCs from young and old C57BL/6 mice were sorted into 2% BSA (PBS) and directly subjected to a modified ATAC-Seq protocol (PMID: 27526324). Tagmented DNA was pre-amplified using 5 PCR cycles using barcoded Nextera PCR primers and the optimal numbers of cycle (30% of max) was determined by qPCR. After the second PCR, the DNA was purified by a MinElute PCR purification coumn (Qiagen) and subsequently size-selected by Ampure beads to obtain fragments between 50-1000bp.