GSM5322016: miRNA_VU3; Homo sapiens; miRNA-Seq
Tissue samples were homogenized with the TissueLyser LT (Qiagen) and total RNA was isolated using the miRNeasy Mini kit (Qiagen) or Trizol reagent (ThermoFisher Scientific). RNA quality and quantity were determined by using Agilent 2100 Bioanalyzer (Agilent Technologies) and Nanodrop 1000 (Thermo Fisher Scientific Inc.), respectively. The small RNA sequencing libraries were constructed using 3 μg total RNA per sample and NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB, USA.) following manufacturer's recommendation. The library preparations were sequenced on an Illumina Hiseq 2500 platform (Illumina, Inc.) using single-end 50bp reads and all samples were run side by side. single-end 50bp