GSM5591883: platelet baseline WT rep3; Mus musculus; miRNA-Seq
purification of platelets was performed using immune-magnetic negative selection to completely deplete leukocytes. Total RNA including miRNA was isolated from murine platelets using the QIAsymphony RNA Kit. The sequencing library was prepared using the QIAseq miRNA Library Kit with 10 to 20 ng input. The libraries were pooled in an equimolar fashion and sequenced on a NovaSeq6000 as paired-end reads with a sequencing depth of >12 Mio clusters (i.e. read-pairs) per sample.