GSM5942981: Chondro; Homo sapiens; RNA-Seq
RNA was extracted using TriPure TRIzol reagent. Chloroform was added in a 1:5 ratio by volume and phase separation was performed. The RNA-containing layer was mixed with an equal volume of 100% isopropanol and incubated overnight at -20 °C for precipitation. RNA was pelleted and washed with 80% ethanol and resuspended in nuclease-free water. Library preparation was performed using Illumina TruSeq Stranded mRNA Low-Throughput Sample Prep Kit (Cat# 20020594) according to manufacturer's instruction.