GSM5057572: HR51_24h_C; Drosophila melanogaster; RNA-Seq
Brains were dissected a cold Ringer's solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted  into cDNA and linearly amplified by T7 in vitro transcription. 3' MARseq Jaitin et al, 2014