GSM5799439: hair follicles of 5G groups (5G dose irradiation, collect organ-cultured HFs for 3 days); Homo sapiens; RNA-Seq
After collecting hair follicle tissues in different treatments, total RNA was isolated from the tissues using the Trizol (invitrogen) as soon as possible. RNA purity was assessed and each RNA sample had an A260:A280 ratio above 1.8 and A260:A230 ratio above 2.0. RNA integrity was evaluated using the Agilent 2200 TapeStation (Agilent Technologies, USA) and each sample had the RIN above 7.0. rRNAs were removed from Total RNA using EpicentreRibo-Zero rRNA Removal Kit (illumina, USA). Subsequently, the purified RNAs were subjected to first strand and second strand cDNA synthesis following by adaptor ligation and enrichment with a low- cycle. The purified l library products were diluted to 10 pM for cluster generation in situ on the pair-end flow cell followed by sequencing (2×150 bp) HiSeq3000. RNA libraries were prepared for sequencing using standard Illumina protocols