NextSeq 550 paired end sequencing; RNA-seq of SMARCD1 knockdown in U937 leukemic cell line in untreated and vitamin D3 treated condition
U937 cells were procured from ATCC and maintained in RPMI (GIBCO), supplemented with 10 % FBS (GIBCO). Total RNA was extracted using RNA isolation kit (Zymoresearch) following manufacturer's protocol. Quality and concentration was checked using a spectrophotometer (Multiskan GO, Thermo Scientific). Quality of RNA was assessed by NanoDrop™ (Thermo Scientific) and Tapestation (Aligent Technologies). RNA was quantified in a Qubit 3 Fluorimeter, using Qubit RNA HS Assay Kit 9thermo Scientific. 800 ng of total RNA with RIN values ≥ 8 were selected for library preparation using TruSeq™ stranded mRNA kit (Illumina) following manufacturer's instructions.