GSM4745619: Ventricle; Mus musculus; OTHER
FFPE samples of adult mouse and mouse embryo were obtained from Zyagen (San Diego, CA). According to Zyagen protocol, the mice used in this project were purchased from Charles River Laboratories. Adult mice were sacrificed upon arrival, and the aorta, atrium and ventricle were collected. The embryo (E10.5) were collected the day the pregnant mouse was received. The FFPE tissues were processed following standard protocols, which includes fixation (10% formalin), dehydration (ethanol series: 70%-100%), clearing (100% xylene), paraffin infiltration and embedding. FFPE sample was sectioned with a thickness of ~10 µm and placed onto a poly-L-lysine coated glass slide. After receiving the sectioned FFPE slides, the tissue sections were stored at -20 °C in a sealed bag until use. Sequencing library was built using standard Nextera kit from Illumina