GSM5918569: C4-2 cells (knockdown control) MeRIP-seq m6A IP (replicate 2); Homo sapiens; RIP-Seq
MeRIP-seq was conducted in duplicates using Magna MeRIP™ m6A Kit (Millipore) with the procedure provided by the manufacturer. In brief, the total RNA from C4-2 and PrEC cells was extracted using RNeasy Plus Mini Kit (Qiagen), followed by mRNA isolation using PolyATtract mRNA Isolation System (Promega). The obtained mRNA was fragmented into ~100 nt in length and purified by miRNeasy Mini Kit (Qiagen). After incubation with anti-m6A antibody-magnetic beads mixture at 4 °C overnight, the m6A-modified mRNA fragments bound to beads were eluted and shipped to BGI for library preparation and sequencing using the DNBseq platform. Samples were sent to BGI for library preparation and follow-up sequencing MeRIP-seq