GSM5668079: Bat3_Putamen_atac; Rousettus aegyptiacus; ATAC-seq
For ATAC-seq, nuclei were extracted from cryopreserved tissue with Dounce homogenization in lysis buffer. The nuclei were pelleted by centrifugation and filtered through 70µm and 40µm strainers. For RNA-seq, total RNA was purified from flash-frozen tissue using the Qiagen RNeasy Mini Kit. ATAC-seq libraries were constructed with standard amplification and size selection methods. RNA-seq libraries were prepared using NEBNext RNA Library Prep Kit with Poly(A) Selection, selecting for 300bp fragments. See publication for more details.