GSM5668293: Control 3; Homo sapiens; RNA-Seq
The total RNA was extracted using KAPA RNA HyperPrep Kit as per manufacturer instructions. Quality and quantity of RNA were checked using a 2100 Bioanalyzer (Agilent Technologies, CA, USA) and the samples with an RNA integrity number ≥ 7 were sequenced. Ribo-depleted RNA was used for Library preparation and paired end sequencing was performed using Illumina platform (Nextera sequencing) and sequencing chemistry according to the manufacturer's instructions (Life Technologies, USA).