GSM5668022: CD27 negative MAIT cells of Diabetic obese patient 1# [P1_CD27_neg_805D74]; Homo sapiens; RNA-Seq
CD27 positive or negative MAIT cells were stained and sorted as CD27+ or CD27- CD3+ CD161+ TCR Va7.2+ on the BD FACSAria III cell sorter (BD Biosciencesa). RNA from 10,000 sorted cells was used to prepared RNA-seq library according to the single cell Smart-seq2 protocol. RNA libraries were prepared for sequencing using Tn5 based DNA prepration Kit (TD503, Vazyme).