GSM4746095: sapx tapx pgr5 rep1; Arabidopsis thaliana; RNA-Seq
Total RNA extracted as describe above was purified using NucleoSpin RNA plus (Takara). Total RNA was fragmented and converted to double-stranded DNA using TruSeq RNA Sample Preparation Kit v2 (Illumina). Quality and quantity of the resulting library were assessed by using a Bioanalyzer and a KAPA library quantification kit (KAPA Biosystems), respectively.