GSM5941074: H3K27ac_ChIP-Seq; Homo sapiens; ChIP-Seq
Lysates were clarified from sonicated HSF1/H3K27ac-DNA complexes were isolated with antibody. 10 ng DNA of each sample was converted to phosphorylated blunt-ended; An 'A' base was added to the 3' end of the blunt phosphorylated DNA fragments; Illumina's genomic adapters were ligated to the A tailed DNA fragments; PCR amplification was performed to enrich ligated fragments. The enriched product of ~200-1500 bp was size selected using AMPure XP beads.