GSM5322047: mRNA_Wound1_4; Homo sapiens; RNA-Seq
The mRNA libraries were constructed with a total amount of 2 μg RNA per sample. Firstly, the ribosomal RNA was depleted by Epicentre Ribo-zero® rRNA Removal Kit (Epicentre). Subsequently, bulk strand-specific total-transcriptome RNA sequencing libraries were prepared by incorporating dUTPs in the second-strand synthesis step with NEB Next® UltraTM Directional RNA Library Prep Kit for Illumina® (NEB). Finally, the libraries were sequenced on an Illumina Hiseq 4000 platform and 150 bp paried-end reads were generated for the following analysis. paired-end 150bp