GSM5669735: STICR_Lentiviral_Library; Synthetic plasmid; RNA-Seq
Unconcentrated lentiviral-laden media was filtered using a 0.45micron filter and then concentrated by ultracentrifugation. Viral pellet was resuspended in 100ul sterile PBS. 5ul of concentrated lentivurs was added to Trizol and RNA was exctracted RNA was reverse transcribed using a primer targeting the lentiviral transcript, but not anti-sense transcript, to made cDNA. Individual cDNA molecules were tagged with a UMI using a primer during second-strand synthesis. PCR was then perofrmed to amplify these products and  add Illumina sequencing adapters.