GSM5592178: Lung, SH.TBL15I; Homo sapiens; RNA-Seq
Granuloma TB lesions were removed and ~0.5 cm3 biopsy fragments of tissue samples  were kept on RNAlater solution (Qiagen) at 4ºC overnight for RNA-seq. Fresh tissue samples intended for RNA-seq were removed the following morning, for finally being stored at -80ºC. The mirVana miRNA Isolation Kit (Thermo Fisher Scientific) allowed the total-RNA isolation and recovery, followed by a genomic DNA digestion through DNA-free DNA Removal Kit (Thermo Fisher Scientific) considering manufacturer's instructions, respectively. Total-isolated-RNA was diluted to reach a 25 ng/µl aliquot. Libraries were constructed by the TruSeq Stranded Total RNA LT Sample Prep Kit (Human Mouse Rat) (Illumina) following the TruSeq Stranded Total RNA Sample Prep guide (Part #15031048 Rev. E), including a prior removal of ribosomal RNA through the RNA Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat) (Illumina). RNA-seq was performed on an Illumina platform HiSeq 4000 (Illumina), at 50 million reads per sample, 100bp stranded paired-end reads