GSM6127707: RNA_HEC_WT_rep2; Homo sapiens; RNA-Seq
Total RNA was isolated from the fresh tissues with RNeasy Plus Mini Kit (Qiagen) according to manufacturer's instructions and treated with RNase-free DNase. For RNA-seq, 100ng total RNA in each sample are used for SMART-seq2 library construction.For ATAC-seq, ATAC-seq library was prepared followed the omini-ATAC-seq protocol. For ChIP-seq, ChIP-seq library was prepared followed the ULI-ChIP protocol. For CUT &Tag, 50000 fresh cells were subjected to library construction.