GSM5525600: Healthy Donor 2 PBMCs; Homo sapiens; RNA-Seq
We isolated PBMCs from whole blood using BD Vacutainers (BD, Franklin Lakes, NJ, USA) according to the manufacturers' instructions. We aimed for 6,000 cells per sample. Libraries were prepared using the Chromium controller (10X Genomics, Pleasanton, CA; USA) in conjunction with the single-cell 3' v2 kit (1st experiment, stable state) or single-cell 3' v3 (2nd experiment, stable state; acute state) according to the manufacturer's instructions.