GSM5861189: BT273 shRNA18-2; Homo sapiens; RNA-Seq
Total RNA was prepared from the GSCs using the Direct-zol™ RNA MiniPrep kit (Zymo Research). The RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer's instructions (New England Biolabs, Ipswich, MA, USA), at GENEWIZ Germany GmbH (Leipzig, Germany).