GSM5668196: MDSC_Shp2f/f_2; Mus musculus; RNA-Seq
Total RNA was isolated from the cells using the Qiagen RNeasy Mini Kit (Qiagen, cat# 74104). 400ng of total RNA was then used in Illumina's TruSeq Stranded mRNA Library kit (Cat# 20020594) for polyA mRNA isolation and library construction. Libraries were sequenced on Illumina NextSeq 500 as paired-end 42-nt reads. Sequence reads were analyzed with the STAR alignment – DESeq2 software pipeline