GSM5321694: J5DD-1; Caenorhabditis elegans; RNA-Seq
Young adults grown at 25°C at F3 generation were collected and washed in M9 to remove residual bacteria, flash-frozen in liquid nitrogen and stored at -80°C before RNA extraction. RNA was isolated from 4 independent cultures for each strain using TRIzol reagent and RNase-Free DNase Set. Sequencing libraries was constructed using TruSeq RNA Library Prep Kit v2. Libraries for sequencing were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB E7645S) and Multiplex Oligos for Illumina (NEB E7335S, E7500S). Quality and size of the libraries were checked by Bioanalyzer (Agilent 2100 Bioanalyzer). 