GSM5669822: H3K27ac_ChIP1_Dox_Untreated_rep2 [722]; Mus musculus; ChIP-Seq
ChIP assays were performed using the ChIP assay kit (Millipore) following the manufacturer's protocol as previously described (Ridinger-Saison et al., NAR, 2012). ChIP samples were sonicated for 17min using the Covaris S220 sonicator (peak power 220, duty factor 20 and cycle Burst 200) was used for sonication. ChIP samples were purified using Agencourt AMPure XP beads (Beckman Coulter). ChIP-seq libraries were prepared from 5ng of double-stranded purified DNA using the MicroPlex Library Preparation kit v2 (C05010014, Diagenode s.a.).