GSM6131589: BE2C-HDAC2-dTAG, H3K27ac, DMSO (0.1%), 2 h; Homo sapiens; OTHER
Cells were permeablized with 0.02% digitonin. CUT&RUN fragments were extracted following the protocol based on Skene&Henikoff, Nat Protoc. 2018. and resuspended in 13 µL of H2O. CUT&RUN libraries were prepared with Takara ThruPLEX DNA-Seq Kit and barcoded with Takara DNA Unique Dual Index Kit. 10 µL of input material was used for library preparation. The libraries were then amplified with 16 cycles of 98° C for 15 s and 60° C for 10 s on thermocycler to allow prefrential amplification of the small CUT&RUN fragments. Libraries were then purified with AMPure XP beads and validated on Agilent TapeStation.