GSM5012472: AEC-FT_rep2; Homo sapiens; RNA-Seq
Total RNA was extracted and purified using the Illustra TriplePrep kit (GE Healthcare 28-9425-44) Purified total RNA was submitted to the USC Molecular Genomics Core facility for library preparation and sequencing. RNA quality was assessed on the Bioanalyzer (Agilent) then rRNA-depleted using Ribo-Zero rRNA Removal kit for human samples (Illumina MRZH11124) before proceeding with library preparation (TruSeq mRNA Stranded Library preparation kit, Illumina 20020594).