GSM4904645: C2C12-G2M-input, 2; Mus musculus; ChIP-Seq
Total RNA was obtained from the C2C12 cells using Sepasol-RNA I Super G (Nacalai Tesque Inc.) and ethanol precipitation. Sequencing was performed in an Illumina Illumina HiSeq1500 system with 101 cycles for both read 1 and read 2 using HiSeq PE Rapid Cluster Kit v2 and  HiSeq Rapid SBS Kit v2 (200 Cycles) for RNA-seq, and with 51 cycles for read 1 using TruSeq SBS Kit v3-HS (50 Cycles) and TruSeq SR Cluster Kit v3-cBot-HS for ChIP-seq. C2C12 cells were sorted into three groups on the basis of the cell cycle (G1, S, and G2-M phases) and used for RNA-seq analysis. The C2C12 cells in the three groups (G1, S, and G2-M phases) were used for the ChIP-seq analysis.