GSM5984097: NIH3T3 cells, EV_MIY-D2_F3; Mus musculus; RNA-Seq
Libraries were constructed using a bulk RNA-seq method named Prime-seq, which uses direct cell lysis, bead cleanup, cDNA synthesis and pre-aplification with KAPA HiFi Polymerase (see Janjic et al, 2021) The cells were counted by Vi-Cell XR (Beckman Coulter, Brea, CA, USA) and 1x104 cells were harvested in microtubes containing 100 µl of DPBS 1x. After 5 minutes of centrifugation at 1500 rpm, cells were resuspended in 100 µl of Buffer RLT Plus, RNeasy Plus Lysis Buffer (Qiagen, Hilden, Germany) supplemented with 1% b-mercapoethanol. Prime-seq (Janjic et al, 2021 -  https://doi.org/10.1101/2021.09.27.459575)