GSM5940251: Liver ChIP-seq input O2; Mus musculus; ChIP-Seq
Liver tissue was crosslinked with 1% formaldehyde, dounce homogenized and the crosslinking reaction was quenched with 0.125 M glycine. Cross-linked tissue was lysed, nuclei pellets washed and then sonicated. For immunoprecipitations, samples were incubated with the respective antibody overnight and purified using magnetic protein G Dynabeads. Crosslinks were reversed and DNA was purified using the Nucleospin Gel and PCR Clean up kit (Macherey-Nagel) Libraries were prepared as described in Tessarz et al, 2014