GSM5918746: PTB187-EVs; Homo sapiens; RNA-Seq
The frozen brain tissue was chopped on ice using a scalpel to generate 5 mm3 sections.EVs are extracted by using a SEC column (qEV original, Izon).Total RNA was extracted from isolated EVs using the RNeasy Micro Kit (74004, Qiagen) according to the manufacturer's instructions. The RNA concentration and purity were measured with Qubit 3.0 (Thermo Scientific) and Agilent 2100 Bioanalyzer. RNA sequencing was performed using the Illumina HiSeq 3000 platform. Sequencing libraries were prepared using Total RNA-seq Library Prep kit from Illumina. RNA libraries were prepared for sequencing using standard Illumina protocols