GSM5591852: NHDF-1; Homo sapiens; RNA-Seq
Total RNAs were obtained from fibroblasts using Trizol reagent (Invitrogen Life Technologies, Grand Island, NY). Poly(A) mRNA isolation from the total RNA and subsequent fragmentation were performed using TruSeq RNA Sample Prep Kits v2 (Illumina, San Diego, CA, USA), according to the manufacturer's instructions. RNA libraries were prepared for sequencing using standard Illumina protocols. The adaptor ligated libraries were sequenced using an Illumina NovaSeq 6000.