GSM5127807: WT4; Mus musculus; RNA-Seq
Bone marrow extracted from mice was treated with 1:20 CMG14-12 conditioned media (containing M-CSF) to generate bone-marrow derived macrophages. Macrophages were treated with 100 U/ml IFNgamma for 16 h followed by 100 ng/ml LPS for 6 hours to induce M1 activation. RNA was prepared using OMEGA Bio-tek EZNA on-column isolation kit. Libraries were constructed using the Illumina Stranded mRNA library preparation kit to generate single-end reads 1x75bp on NextSeq High Output Runs.