GSM5625663: sgCXCR4_CEM207_input_Rep1; Homo sapiens; ChIP-Seq
Chromatin bound to BRD4 was immunoprecipitated from fixed and sheared nuclei, with or without CEM207 treatment, in HCT116 cells bearing sgNT or sgCXCR4 (promoter). Enriched DNA from ChIP was barcoded and libraries were amplified, followed by size selection (fragment ranges between 250 and 1,000 bp), using NEBNext® Ultra™ II DNA Library Prep with Sample Purification Beads (E7103S) and NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs) (E6440S). Libraries were sequenced to a depth of 30 million base pairs (75-bp single-end reads) on an Illumina NextSeq 500.