GSM5320873: RNA-seq ESC KO+Smad7 rep2; Mus musculus; RNA-Seq
Total RNA was extracted using TRIzol reagent (Invitrogen), according to the manufacturer's protocol. Approximately 2 μg of total RNA were subjected to poly(A) selection, and libraries were prepared using the TruSeq RNA Sample Prep Kit (Illumina) following manufacturer's instructions. Sequencing was performed on the Illumina NextSeq 500 platform.