GSM5592749: Bone Marrow Derived Macrophage -Cigarette Smoke exposure 1; Mus musculus; RNA-Seq
Total RNA was purified from bone marrow cells or BMDMs using Direct-zol RNA MiniPrep Kit (Zymo Research) and TRIzol reagent (Ambion) according to the manufacturer's instructions. Total RNA was used to generate libraries using a NEXTflex Rapid Directional RNA-Seq kit (Bioo Scientific, $5138-07) according to the manufacturer's instructions. Samples were quality assessed using TapeStation (Agilent, Santa Clara, CA) and read on a High-Seq System (Illumina, San Diego, CA.