GSM5128896: AuCG_N; Triticum turgidum subsp. durum; miRNA-Seq
Total RNA was extracted from frozen seedling shoot material using the Tri reagent as previously described (Sigma-Aldrich, North Ryde, Australia). Extracted total RNA samples were treated with TURBO DNase (ThermoFisher Scientific, Scoresby, Australia) following the manufacturer's instructions. The concentration, quantity and quality of purified total RNA samples were measured on a NanoDrop Lite spectropho-tometer (ThermoFisher Scientific, Scoresby, Australia). RNA integrity was assessed by agarose gel electrophoresis and Bioanalyzer measurement. High-quality RNA samples from the six biological replicates per treatment group were equally pooled for small RNA sequencing purpose. High-quality RNA samples from the six biological replicates per treatment group were equally pooled for small RNA sequencing purpose. A total of eight small RNA libraries (AuCG_C, AuCG_N, AuWH_C, AuWH_N, L6CG_C, L6CG_N, L6WH_C, L6WH_N) were made using the TruSeq Small RNA Sample Prep Kit Small RNA sequencing (single-end, 50 bp) was performed on an Illumina NovaSeq 6000 at LC-Bio (Hangzhou, China).