GSM5123998: BRG_KO1_REP2; Homo sapiens; RNA-Seq
RNA was extracted from cells with the RNeasy Mini Kit (Qiagen). The Dana-Farber Cancer Institute Molecular Biology Core Facilities prepared libraries from 500 ng of purified total RNA, using RNA sequencing libraries were generated using the NEB Next Ultra RNA Library Prep Kit (NEB #E7760).  according to the manufacturer's protocol; finished libraries were quantified by the Qubit dsDNA High-Sensitivity Assay Kit (#32854, Thermo Fisher Scientific), by an Agilent TapeStation 2200 system using D1000 ScreenTape (# 5067-5582, Agilent), and by RT-qPCR using the KAPA library quantification kit (# KK4835, Kapa Biosystems), according to the manufacturers' protocols; pooled uniquely indexed RNA-seq libraries in equimolar ratios, and sequenced these to a target depth of 40M reads on an Illumina NextSeq500 run with single-end 75bp reads.