GSM5590528: S28_HDM; Homo sapiens; RNA-Seq
For bulk RNA-seq RNA was isolated using Direct-zol RNA micro prep (Zymo, UK) as per the manufacturer's protocol. For Bulk RNA-seq cDNA libraries were generated using SMART-Seq Stranded Library Preparation for Ultra Low Input according to the SMART-Seq Stranded Kit User Manual  following the Ultra low input workflow (Takara Bio). Samples were pooled (12/batch) for library preparation. For Drop-seq High Sensitivity DNA Assay Nextera kit, Illumina, was used. For Constellation cDNA was aplified using targeted linear amplification (20 rounds) and further prepared using True-seq, Illumina.