GSM5828442: EU286_YP2; Homo sapiens; RNA-Seq
Total RNA was extracted from the non-infected and infected macrophages using the miRNeasy kit (QIAGEN). RNA quantity was evaluated spectrophotometrically, and the quality was assessed with the Agilent 2100 Bioanalyzer (Agilent Technologies). Only samples with no evidence for RNA degradation (RNA integrity number > 8) were kept for further experiments. RNA- sequencing libraries were prepared using the Illumina TruSeq protocol.