GSM6127790: WBC.Cluster1; Homo sapiens; RNA-Seq
The cells were lysed using RLT+BME buffer. RNA was extracted from the lysed samples using the Macherey-Nagel Nucleospin RNA XS kit. Double stranded cDNA was generated using the Takara Bio SMART-Seq v4 ultra low input RNA kit for sequencing. Barcoded Illumina compatible sequencing libraries were generated using the Illumina Nextera XT DNA library preparation kit with a reduced tagmentation time of 3 minutes and the samples were cleaned using a 1:1 bead ratio to eliminate the presence of primer dimers.