GSM4746354: whiteRNAi_Rhino_input_CHIPseq_ovary; Drosophila melanogaster; ChIP-Seq
Ovary was dissected in PBS. Then crushed in 1.8% formaldehyde for 15 mins. Sonication was performed in the Covaris E220. ChIP was performed by incubating the sheared chromatin overnight with the antibody at 4C. Decrosslinking was performed overnight at 65C. DNA was isolated using the Zymo ChIP DNA kit.