GSM5829795: Patient sample 312A; Homo sapiens; Bisulfite-Seq
Genomic DNA was isolated (PureLink Genomic DNA Kit, K1820-01) from whole blood (separately we biobanked buffy coat containing leukocytes and platelets at -80ºC), subjected to enzymatic digestion with MspI (NEB, R0106S) and bisulfite converted (EpiTect Fast. DNA Bisulfite Kit, Qiagen, 59824). Samples underwent library preparation involving unique adaptor ligation and were subsequently pooled to be individually sequenced together. Single stranded DNA was then isolated and hybridized with 120 bp biotinylated DNA probes from Integrated DNA Technologies (Coralville, IA). Following probe pull down with streptavidin magnetic coated beads, the pooled samples underwent bisulfite conversion, PCR amplification and sequencing on NovaSeq 6000 instrunments. Bisulfite-seq (targeted)