GSM5940108: female_BM_neutrophil; Mus musculus; RNA-Seq
All murine tissues in this study were prepared from mice sacrificed by cervical dislocation at the same time. For the isolation of bone marrow cells, leg bones from murine femur were flushed using a 21-gauge needle in RPMI1640 medium containing 1% FBS. single-cell suspension of bone marrow were stained with fluorophore-conjugated antibodies (APC-conjugated-anti-CD45 and FITC-conjugated-anti-Gr1) for 30 min on ice. Fluorescence-activated cell sorting (FACS) was performed on a Moflo-XDP system (Beckman-Coulter). FACS-sorted viable cells were loaded into a well in a microfluidic chip to generate a cDNA library using a droplet-based sequencing platform (10× Genomics). Libraries were constructed using the Single Cell 3' Library Kit V2 (10× Genomics).