GSM5942930: Tyw1_WT_1_3_repl1; Saccharomyces cerevisiae; RNA-Seq
RNA was extracted with Trizol, deacylated and annealed to a double-stranded adapter with RNA ligase 2. Reverse transcription was performed with Superscript IV, RNA hydrolyzed and the cDNA precipitated then PAGE purified. After precipitation, the cDNA was circularized with CircLigase (Epicentre) and amplified with the NEBnext Ultra II Q5 next-generation master mix. Sequencing was performed as single-end reads for 110 cycles on a NextSeq 550 (v2.5)