GSM5668147: pDCRNA8640; Homo sapiens; RNA-Seq
PBMCs were extracted over Ficoll-Paque using established methods. pDCs were isolated using Miltenyi Biotec Human Plasmacytoid Dendritic Cell Isolation Kit II (130-097-415) in accordance with the manufacturer's protocol. Libraries were prepared using the Illumina TruSeq stranded Library kit and sequenced on a NextSeq 500 following manufacturer's instructions.