GSM5127723: GMC_BL6J_14M_ET20_c636_spleen; Mus musculus; RNA-Seq
Mouse tissues were harvested, flash frozen on dry ice and total RNA isolated from the tissues with TRI-Reagent. Total RNA was purified with the Monarch RNA Cleanup kit.  Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using a ligation mediated RNA sequencing protocol published by Huo et al. 2015