GSM5124308: AD-MSCs_cloupe 2; Mus musculus; RNA-Seq
Forty-eight hours after MSC or sEVs injection, mice were euthanized, cirrhotic livers were perfused with PBS, and then harvested and dissociated into single cells using the liver dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer's protocol. The dissociated cells were sorted using Mofl XDP (Beckman Coulter Inc.) after propidium iodide (PI) staining (1 µg/ml) to exclude dead cells. Cells were prepared as scRNA-seq library using Chromium Single Cell 3' Reagent Kits v3 (10x Genomics, Pleasanton, CA, USA).