GSM4746064: Sample_139350: shRNA_2bp_rep4_miRNAseq; Mus musculus; miRNA-Seq
TRIzol isolation + miRNeasy Purification Samples were prepared using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (catalog number E7300L). Adapters were ligated to 100ng of total RNA which then goes through 1st Strand Synthesis and 15 cycles PCR amplification. The products are purified and size selected by Pippin Prep according to NEB protocol recommendations.  Final libraries were checked for quality and quantity by TapeStation (Agilent) and qPCR using Kapa's library quantification kit for Illumina Sequencing platforms (catalog # KK4835) (Kapa Biosystems,Wilmington MA)