GSM4744971: EnzNeg_rep3; Homo sapiens; OTHER
QIAamp DNA Micro Kit (QIAGEN, Cat.# 56304) The detailed library construction protocol by Cellecta is found in the RNAi Pooled Lentiviral shRNA Libraries User Manual, section 6.2. Briefly, custom PCR primers are used that specifically bind to the Decipher vector at sequences flanking the shRNA-specific barcode sequence, which had integrated into the genome. A second round of PCR is performed to add Illumina P5 and P7 sequencing primers onto the barcoded amplicons. PCR products were then purified by gel electrophoresis before deep sequencing. Amplification of Cellecta barcode sequences from gDNA