GSM5798827: T021518_TRM [GEX]; Homo sapiens; RNA-Seq
Tissue pieces from the ovarian tumors were mechanically dissociated and single cell suspensions were prepared after debris removal. TRM and re-circulating CD8+ TILs were FACs sorted in Aria-sort The scRNA-Seq libraries were prepared following the protocol provided by the 10X genomics. The cDNA and VDJ-enriched libraries were completed in a single bulk reaction using the 10X Genomics Chromium NextGEM Single Cell 5' v 1.1 and V(D)J Reagent Kits.  50,000 or 5,000 sequencing reads per cell for scRNA-seq or VDJ libraries, respectively, were generated on the Illumina NextSeq 500 instrument. 10X genomics Chromium 5' Single Cell RNA-seq + VDJ Single Cell T Cell Receptor Sequencing