GSM5592442: B6-rep3-stimulated; Mus musculus; RNA-Seq
Total RNA was extracted using RNeasy mini kit (Qiagen: 74104 ) following the instruction of the manufacturer and quantified. 1ng of RNA was used in triplicates for the synthesis of cDNA using the Superscript III reverse transcription system (ThermoFisher: 18080093). cDNA quality was checked using High sensitivity DNA Bioanalyzer chip (Agilent) and 0.15ng cDNA was subjected to the process of making an amplified library and sequenced on  HiSeq 2500 (Illumina) to generate Smart-Seq2 sequencing data