GSM4745969: naive.CD8.media.4h 3; Mus musculus; RNA-Seq
For RNA-seq, RNA was isolated from sorted P14 T cells at the indicated time points using the Zymo RNA miniprep kit (Zymo Research), and 500 ng RNA was used for RNA-Seq library preparation with the Kapa mRNA HyperPrep Kit (KK8580, Kapa Biosystems) and indexed with NEXTflex DNA Barcodes-24. After the final amplification, samples were loaded onto 2% E-Gel pre-cast gels (ThermoFisher), and 250 to 400 bp DNA fragments were excised and purified with Zymoclean Gel DNA Recovery Kit (Zymo Research). After quantification by Qubit (Invitrogen), barcoded samples were mixed and sequenced on an Illumina HiSeq3000 system.