GSM5766204: org_CD63-LC p4; Homo sapiens; RNA-Seq
Cell recovery was used to extract the organoids from the matrigel domes. Organoids and EDMs were lysed with RNA lysis buffer following the manufacturer's instructions for the Zymo Research Quick-RNA MicroPrep Kit. RNA sequencing libraries were generated using the Illumina TruSeq Stranded Total RNA Library Prep Gold with TruSeq Unique Dual Indexes (Illumina, San Diego, CA). Samples were processed following manufacturer's instructions, except modifying RNA shear time to five minutes. Resulting libraries were multiplexed and sequenced with 100 basepair (bp) Paired End (PE100) to a depth of approximately 25-40 million reads per sample on an Illumina NovaSeq 6000. Samples were demuxltiplexed using bcl2fastq v2.20 Conversion Software (Illumina, San Diego, CA).