GSM4745280: Duodenum_3; Callithrix jacchus; RNA-Seq
Necropsy samples were flash frozen in liquid nitrogen and stored at -80C. Tissue was homogenized with a pestle and extracted using TRIzol. Total RNA was shipped to Arraystar. Total RNA was depleted of rRNA. Illumina kits were used for the RNA-seq library preparation including RNA fragmentation, random hexamer primed first strand cDNA synthesis, dUTP based second strand cDNA synthesis, end-repairing, A-tailing, adaptor ligation and library PCR amplification.