GSM6128283: WT, untreated, biol replicate 2, scRNAseq; Mus musculus; RNA-Seq
Untreated and treated colonid were subject to cell dissociation with TrypLE to generate single cell suspension. Live cell % had to exceed 65% to load suspension into 10X Chromium gel beads Library construction was performed according to 10X Genomics v3 chemistry kits suggested protocol.