GSM5940110: priB_Rad21_ChIPseq_Spt5depleted; Mus musculus; ChIP-Seq
ChIPseq and ChIP-qPCR were performed as described without modifications (Pavri et al., 2010). In short, 20x10^6 cells were crosslinked with 1% HCHO for 10 min at 37 degree. After quenching with 0,125 M glycine, cells were sonicated with the Diagenode Bioruptor sonication machine. After chromatin-immunprecipitation, libraries were prepared following the NEB Next Ulta II DNA library kit. Libraries were amplified with the kappa library amplification qCR master mix