GSM4904710: MEP1+Rapamycin; Mus musculus; RNA-Seq
Remaining donor-derived CBir1 TCR Tg CD4+ cells in the spleen were sorted with BD FACSAria and stored in Trizol at -80C until RNA was extracted. RNA extraction, purification, library preparation were performed by Genewiz. RNA libraries were prepared for sequencing using Ultra-low library preparation protocols at Genewiz.