GSM4904518: LNCaP METTL3 sh2 0 DOX R3 Ribo-Seq; Homo sapiens; OTHER
Ribo-Seq: Ribsosome footprints were generated by digestion with RNAseI, followed by collection of ribosome-bound RNA fragments using MicroSpin S-400 columns. RNA fragments were purified using with the RNA Clean & Concentrator-25 kit from Zymo, and rRNA was removed using the NEBNext rRNA Depletion kit. 28-30 bp footprints were further purified on a 15% polyacrylamide TBE/Urea gel. Ribo-Seq: Libraries were prepared with the purified footprints and the SMARTer Small RNA-Seq Kit from Takara following the manufacturer's instructions.