GSM5013288: Colon Lamina Propria CD4 T Cells - IL23R Knockout rep2; Mus musculus; RNA-Seq
Spleens were extracted and then a single-cell suspension was prepared by homogenization with a syringe plunger and debris was filtered with a 70um filter. Red blood cells were lysed with ACK lysis buffer from Lonza. The intestinal T cells were isolated with the lamina propia dissociation kit (cat. # 130-097-410) from Miltenyi Biotec. Cells were encapsulated into droplets and libraries were prepared using Chromium Single Cell 3' Reagent Kits v2 according to the manufacturer's instructions (10x Genomics).