GSM5173211: nt_3_22; Schmidtea mediterranea; RNA-Seq
RNA was isolated using TRIZOL. mRNAseq libraries were generated from 500 ng of high quality total RNA, as assessed using the Bioanalyzer 2100 (Agilent). Libraries were made according to the manufacturer's directions for the TruSeq Stranded mRNA LT– set A and B (Illumina, San Diego, CA; Cat. No. RS-122-2101 and RS-122-2102) and using NEXTflex DNA Barcodes (BiooScientific, Austin, TX; Cat. No. 514104). Resulting short fragment libraries were checked for quality and quantity using a LabChip GX (Perkin Elmer) and Qubit Fluorometer (Life Technologies, Carlsbad, CA).