GSM5940191: mktC_2524_12WRec_2DRas; Mus musculus; RNA-Seq
Single cell suspensions were prepared from pancreata and stained with DAPI. ~300k DAPI(-) cells were sorted and collected via FACS. ~300k DAPI(-) tdTomato(+) cells were sorted and collected via FACS. ~300k DAPI(-) & ~300k DAPI(-) tdTomato(+) were combined into a single tube. 10X Genomics Chromium was employed to add barcoded primer gel beads to each single cell. The captured polyA mRNA from single cells was reversely transcribed, barcoded and amplified following the standard 10X Genomics Chromium Single-cell 3' Library RNA-Seq Assays protocol. Libraries were sequenced on Illumina NovaSeq6000 at the Epigenomics Core Facility at Weill Cornell Medicine.