GSM5525754: Swi6 ChIPseq, clr4wt, rep1; Schizosaccharomyces pombe; ChIP-Seq
ChIP was performed as described in Kuzdere et al. (2021). In brief, exponentially growing or synchronized fission yeast cells were fixed with formaldehyde and lysed by beat-beating. Chromatin was sheared by sonication and the IP was performed for 2h at 4°C. ChIPs were decrosslinked overnight at 65°C and cleaned up using AMPure XP beads. ChIP-seq libraries were prepared with the NEBNext Ultra II DNA Library Prep Kit (NEB, E7645L) according to the manufacturer's instructions.