GSM5012251: M1_12hrs_Rep2_bATACseq; Homo sapiens; ATAC-seq
Omni ATAC protocol(Corces et al., 2017) as previously described. Approximately 50,000 HL-60, macrophages and activated macrophages were collected for bulk ATAC-seq. Briefly, cellular viability was assessed and cells were subsequently washed with PBS prior to library preparation. Bulk ATAC-seq protocol was performed as previously described (Corces et al., 2017) with an added size selection step to enrich for DNA fragments ranging from 150 to 500bp. Cells were lifted using trypsin. Total RNA was extracted by using the RNeasy kit (QIAGEN).