GSM5738855: 15 month male M3; Mus musculus; RNA-Seq
Bladders were collected and snap frozen in liquid nitrogen, total RNA from fresh-frozen bladders was isolated using the RNeasy Mini Kit (Qiagen Inc, Canada). Qualitative and quantitative assessments of RNA were performed using a spectrophotometer (Nanodrop ND-100) and a bioanalyzer (Agilent). Samples that met the purity requirement (>8.0 RNA integrity number) were subjected to bulk RNA-sequencing (Genome Quebec, Montreal, QC, Canada) Libraries were prepared using standard illumina protocols after samples were treated with the Ribo-zero rRNA depletion kit (Illumina)