GSM5320758: CXCR6, CD44 LN cells (D7); Mus musculus; RNA-Seq
Mice were sacrificed and lymph nodes were isolated. Single cell suspnesion was created. Single cell suspensions were staining with antibodies for 30min at 4 degrees and sorted for the desired population using FACS AriaIII. Single cells were sorted using a FACSAria III (BD Biosciences), before being encapsulated into droplets with the ChromiumTM Controller (10x Genomics) and processed following manufacturer's specifications. Transcripts captured in all the cells encapsulated with a bead were uniquely barcoded using a combination of a 16 bp 10x Barcode and a 10 bp unique molecular identifier (UMI). cDNA libraries were generated using the Chromium™ Single Cell 3' Library & Gel Bead Kit v2 or v3 (10x Genomics) following the detailed protocol provided by the manufacturer. Chromium single cell 3´v2 and v3 (10XGenomics)