GSM5668678: DN thymocytes Toxf/f rep3; Mus musculus; RNA-Seq
Thymi were obtained and prepared for FACS sorting, and the pellets of sorted DN2 thymocytes were frozen on dry ice. RNA was extracted using Trizol reagent. The NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #7530) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols