GSM5057941: 2.5 uM cisplatin MDA-MB-468 rep1; Homo sapiens; RNA-Seq
Total RNA was isolated from cell lines using RNA later followed by purification with RNeasy mini kit (Qiagen) using the manufacturer's recommended in-solution DNase digestion. RNA-seq libraries were prepared using Lexogen's QuantSeq 3'mRNA-seq Library Prep Kit (FWD for Illumina) from 200 ng of DNAse I treated total RNA. Libraries were quantified using Qubit 2.0 dsDNA HS kit and the size distribution determined on a Fragment Analyzer.