NextSeq 500 sequencing; RNA-seq of Sting overexpressing orthotopic pancreas tumors
Pancreatic tumors were harvested from PKCY littermates. RNA was isolated and DNase-treated using the AllPrep DNA/RNA Mini Kit (Qiagen).  Indexed sequencing libraries were generated with the KAPA mRNA HyperPrep Kit (KAPA), quantified and pooled using the TapeStation 4200 (Agilent).