Illumina HiSeq 2500 sequencing; RNA-seq of mouse barrelette neurons at embryonic day 14.5
Cells were dissociated by papain, filtered and kept on ice until FACS sorting. Sorted cells were collected into ice cold PBS 1X, and immediately pelleted by centrifugation (500 rcf, 5 minutes, 4°C).  Total RNA was extracted by NORGEN Single Cell RNA Purification Kit (NORGEN, 51800) with genomic DNA digestion using RNase-Free DNase Ⅰ Kit (NORGEN, 25710) according to manufacturer's protocol.  Sequencing of total RNA was done by Ovation SoLo RNA-seq System (NuGEN, 0501-32), using 2.5 – 5ng of RNA as an input according to manufacturer's protocol.