GSM5799462: OPBT75-Biological replicate 2 [18170XR.01.14]; Homo sapiens; RNA-Seq
RNA isolation was performed with the Qiagen RNA Kit (Qiagen, Hilden, Germany). Sample quality was assessed using Agilent RNA 6000 Nano Reagent on Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA) and quantified by Qubit RNA HS assay (ThermoFisher, Waltham, MA). Ribosomal RNA depletion was performed with Ribo-zero rRNA Removal Kit, HMR (Illumina Inc., San Diego, CA). Library preparation was performed with the NEBNext Ultra II Non-directional Synthesis Module (New England Biolabs, Ipswich, MA). Average final library size is between 400-430 bp. Illumina 8-nt dual-indices were used for multiplexing. Samples were pooled and sequenced on Illumina NextSeq High Output sequencer for 75 bp read length in single-read mode, with an output of 50 million reads per sample.