GSM5766319: DK21066B_01; Homo sapiens; RNA-Seq
Total RNA was extracted with isogen reagent  (Nippon Gene, Tokyo, Japan)  according to the manufacturer's protocol. Libraries were prepared according to the manufacturer's instructions accompanying the Quant Seq 3' mRNA-seq library preparation kit FWD of Illumina (Lexogen, Vienna, Austria). Briefly, an oligodT primer containing an Illumina-compatible sequence at its 5' end is hybridized to the  RNA and reverse transcription is performed. Then, RNA Removal and Second Strand Synthesis were conducted. The libraries were amplified by 12 cycles of polymerase chain reaction (PCR). The purified libraries were captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Illumina NextSeq 500 following the manufacturer's protocols.