GSM5987880: HDF_Cytosol_3; Homo sapiens; RNA-Seq
For RNA extraction, an equal volume of TRIZOL LS reagent (Thermo Fisher Scientific) was added to the cytosol fraction. For the nucleus fraction, 400 μL (24-well plate) or 1000 μL (6-well plate) of QIAzol lysis reagent (QIAGEN) was added to solubilize the pellet. The RNA was purified using a miRNeasy Mini Kit (QIAGEN). Illumina TruSeq Stranded Total RNA Library Prep