GSM5918786: U87MG small RNA-seq; Homo sapiens; ncRNA-Seq
U87MG cell line was procured and maintained in MEM growth media supplemented with 10% FBS and 1% antibiotics Pen-Strep at proper growth condition. Total RNA was extracted from the above cell line using trizol method. The purity of isolated RNA was evaluated using NanoDrop spectrophotometer (Thermo Scientific) by measuring absorbance at 260 nm and 280 nm. The integrity of isolated RNA was checked by running on an Agilent RNA Bioanalyzer to check for the RNA integrity number (RIN), which was found to be 8.7 of small RNAs and 9.3 of total RNAs. For RNA libraries construction, the NEB Next Small RNA Sample Preparation protocol was used. Then, sequencing was done on HiSeq 2500 with a 1x50 bp read size to obtain 100 million  reads. After sequencing, small RNA seq data was processed to create FASTQ files.