GSM5943547: ARV_50X; Homo sapiens; OTHER
gDNA extraction was performed (QIAamp DNA Mini, QIAGEN 51304) and DNA content was determined with Qubit dsDNA HS Kit (Thermo Fisher, Q32854). 500 ng of the gDNA was subjected to DNA library preparation using the NEBNext Ultra II FS DNA Library Prep kit for Illumina (New England Biolabs, E7805S) following manufacturer's instructions (protocol for inputs >100 ng). Fragments were size-selected using AMPure XP beads (Beckman Coulter, 10136224) for fragments of 150-350 bp. Adaptor-ligated DNA was amplified in five cycles by PCR using NEBnext Multiplex Oligos for Illumina (Set1 E7335 and Set2 E75000). For hybrid capture, xGen Gene Capture Pools for the 29 genes of interest were purchased from IDT (see Supplementary Table 1) and 500 ng of DNA was used as input. Hybridization was performed for 16h following the supplier's protocols, including the xGen Universal Blocker-TS Mix (IDT, 1075475) blocking oligos. Post-capture PCR was performed with the NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541S) for 14-20 cycles. Sequencing libraries were quantified using the Qubit dsDNA HS Kit (Thermo Fisher Q32854) and analyzed on an Agilent 2100 Bioanalyzer before sequencing on a HiSeq 4000 (50 bp single-end).