GSM5321348: vehicle_r1; Mus musculus; RNA-Seq
RNA from sorted TAMs was extracted immediately after sorting, using RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. Quality of RNA samples was determined by Bioanalyzer (Agilent). Only samples with RNA integrity number (RIN) of more than 7.5 were used for RNA sequencing. 400 ng RNA was prepared for sequencing using polydT enrichment according to the manufacturer's instructions (Illumina). Library preparation was performed using the NEBNext RNA library prep kit for Illumina. The library quality was assessed using a Fragment Analyzer followed by library quantification using the Illumina library quantification kit.