GSM5057894: Day 0 rep 2 input; Mus musculus; RNA-Seq
Total RNA was isolated from primary mouse keratinocyte with or without calcium-shift induced differentiation. Polyadenylated RNA was further enriched from total RNA using a Dynabeads® mRNA purification kit (Invitrogen). RNA was fragmented using a Bioruptor ultrasonicator (Diagenode) with 30s on/off for 30 cycles.  m6A-IP was performed using the Epimark N6-methyladenosine enrichment kit (New England Biolabs) Library preparation was performed using the Illumina TruSeq Stranded mRNA Sample Prep Kit according to previously published protocols (Dominissini et al., 2012)