GSM5525825: Daudi cells - BioMag - H3K4me3_rep1; Homo sapiens; OTHER
Cells bound to magnetic beads were treated with Digitonine, antibody, then pA-Tn5 fusion transposase. Genomic DNA was isolated and purifired using phenol-Chloroform. Libraries were prepared according to previously reported method (PMID: 31036827). Briefly,  the adapter sequence-tagmented DNA fragments were amplified by PCR using Universal i5 and uniquely barcoded i7 primers and NEBNext High-Fidelity 2x PCR Master mix (New England BioLabs, M0541S) with 14 PCR cycles. The PCR products were purified by using Ampure XP beads, and cleaned with 80 % ethanol. The size distribution of libraries was confirmed by an Agilent 2100 Bioanalyzer Instrument (Agilent Technologies) and High Sensitivity DNA Chips (5067-4626) (Agilent Technologies). The barcoded libraries were quantified by KAPA Library Quantification Kit, Complete kit for ABI Prism, Illumina Platform (KK4835, 07960204001, Roche). The Paired-end sequencing of DNA libraries were sequenced on Illumina HiSeq X sequencer (Illumina) following the manufacturer's protocols.