GSM5056267: HHcy_Ly6c_L1; Mus musculus; RNA-Seq
Flow cytometry sorted CD11b+Ly6G-Ly6Chigh and CD11b+Ly6G-Ly6Clow cells from control and Cbs-/- WBC (200000/MC subset) were collected in 1400 μL QIAzol Lysis Reagent (Qiagen, Germantown, MD) for total RNA extraction. Samples were quality checked on an Agilent Bioanalyzer 2100 using pico RNA chip for RNA integrity number. Total RNA (50-100 ng/sample) were used for cDNA library construction after ribosomal cDNA depletion using Takara pico-input kit. Pooled samples were run for sequencing analysis in duplication on Illumina NextSeq 500 (CT) and Illumina Hiseq 4000 sequencer (HHcy). RNA libraries were prepared for sequencing using standard Illumina protocols