GSM5124191: sample_13 (NP1_05); Mus musculus; RNA-Seq
After electrophysiological recording, the cytoplasmic content of the neuron was harvested into the patch pipette, transferred into lysis solution (RNaseOUT 8 U.µl-1, 2% Triton X-100) and flash frozen. Reverse transcription was performed using a SMART-seq v4 Low Input Kit (Clontech) directly on the cell lysate according to the manufacturer's protocol. After cDNAds amplification, 50 μl of the sample were subjected to cDNA purification on AMPure XP beads. 0.5 ng of each purified cDNAds were used to construct the sequencing library using Nextera XT kit (Illumina) with fragments over 300-bp length from each neuron.