GSM5799181: Stroma from mouse O1S; Mus musculus; RNA-Seq
Total RNA was extracted and purified using RNeasy mini kit (Catalogue No. 74104, Qiagen), following manufacturer's instructions. NEB RNA ultra II kit was used to prepare the bulk mRNA library.  mRNA was first enriched by magnetic beads with oligod(T). mRNA was fragmentated and cDNA was generated via reverse trancription. Illumina adapters were ligated to the cDNAs to make mRNAseq libraries.