GSM5668447: NFO136-8: NFO136-8_HD#8_RNA-Seq; Mus musculus; RNA-Seq
Whole brains were collected as fast as possible from euthanasied mice, bissected longitudinally, then cells were gently dissociated and processed as described in the "overall design" section above. For RNA-Seq, total RNA was extracted using RNAeasy micro kit (Qiagen #74004). For CLIP-Seq, library was constructed following a custom-made protocol (see PMID: 24407355). For RNA-Seq, library was constructed following protocol described chapter 2 of NEBNext Ultra II Directional RNA Library Prep kit for Illumina (NEB #E7760S).