GSM5012354: leave48tt12 rep1; Arabidopsis thaliana; RNA-Seq
Leaf material from plants cultivated under hydroponic conditions was recollected at 3 and 48 hours after initiating the treatments.Leaves were immersed in liquid nitrogen, homogenized to a fine powder, and stored at -80º C. The total RNA of 100 mg of leaf powder for each biological replicate was extracted using the Maxwell® plant RNA kit (Promega Corporation,WI, USA) following the manu-facturer's instructions. RNA libraries were prepared for sequencing using standard Illumina protocols RNA-Seq. Library preparation was perform using Novogene protocol. Sequence reads: 150 bp-Illumina reads, paired-end, unstranded. Number of analyzed reads: 30-47 million pairs.