GSM4745029: Female6_JQ1 input; Mus musculus; ChIP-Seq
Briefly, cells were fixed in 1% formaldehyde prepared in DMEM/F12 and incubated for 10 minutes at room temperature with gentle rocking.  Fixation was quenched by the addition of glycine to a final concentration of 125 mM and incubation for 10 minutes at room temperature with gentle rocking. Cells were then washed with cold PBS and harvested by scraping in 5 ml of cold PBS.  Cells were transferred to 15 mL conical tubes and pelleted with a 5-minute spin (1,000g) at 4ºC. Sonication was performed on an Epishear probe-in sonicator (Active Motif) at 40% amplitude for 8 cycles of 30 seconds with 30 seconds of rest between each cycle. ChIP-seq libraries were constructed as previously described, with an antibody that recognizes H3K27Ac (Active Motif, 39133, rabbit polyclonal). ChIP-seq libraries were constructed with sample-specific barcodes, and pooled before sequencing on a HiSeq 2500 (Illumina).