GSM6131566: A+Input; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a E220 focused-ultrasonicator. ChIP-seq libraries were prepared using the NEBNext® UltraTMII DNA Library Preparation Kit (#E7645S) complemented with NEXTflex DNA Barcodes from Bioo Scientific. 10 ng of DNA was used as starting material for input and ip samples. Libraries were amplified on the thermocycler. Post amplification libraries were size selected at 250-450bp in length using AMPure® XP Beads(Beckman Coulter,#A63881) from Beckman Coulter. Libraries were validated using Qubit Kit.