GSM6127509: Human adjacent liver tissues [B-2b]; Homo sapiens; RNA-Seq
approximately 5000 cells/ FACS-sorted cells were washed with 0.04% BSA DPBS for three times and were resuscitated to a concentration of 700~1200 cells/ul (viability over 85%). Cells were captured in droplets at a targeted cell recovery of cells. Single-cell RNA-seq libraries were prepared using the Chromium Single Cell 3ʹ Reagent Kits v3 (10x Genomics), according to the manufacturer's instructions. Briefly, approximately 5000 cells/ FACS-sorted cells were washed with 0.04% BSA DPBS for three times and were resuscitated to a concentration of 700~1200 cells/ul (viability over 85%). Cells were captured in droplets at a targeted cell recovery of cells. After the reverse transcriptio step, emulsions were broken and Barcoded-cDNA was purified with Dynabeads, followed by PCR amplification. Amplified cDNA was then used for 3'gene expression library construction. For gene expression library construction, 50 ng of amplified cDNA was fragmented and end-repaired, double-size selected with SPRIselect beads, and sequenced on a NovaSeq platform (Illumia) to generate 150 bp paired-end Reads.