GSM5013294: WT2; Mus musculus; RNA-Seq
RNA extraction and sequencing were performed by DNAFORM (Yokohama, Japan).  The qualities and quantities of total RNA were checked by a Bioanalyzer (Agilent, CA, USA) to ensure that the RINs (RNA integrity number) were above 7.0 and the A260/280 and 260/230 ratios were above 1.7. First-strand cDNAs were reverse-transcribed from the RNAs, then second-stranded cDNAs were synthesized and selected by the Cap trapping method. After “bar code” tags were attached, CAGE libraries were constructed. Multiplexed 8 CAGE libraries were sequenced using single end reads of 75nt on an Illumina NextSeq 500 sequencer.