GSM5918834: I17R023f08; Mus musculus; RNA-Seq
Hippocampus and brainstem were removed, flash-frozen on dry ice, and RNA was harvested using the AllPrep DNA/RNA Mini Kit (Qiagen). TruSeq RNA Sample Prep Kit (Illumina) was used with 500 ng of RNA to construct sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols Poly(A)-selected single-end sequencing libraries (75 bp) were generated according to the manufacturer's instructions. All libraries were sequenced on an Illumina HiSeq 2500 platform at a depth of 10–25 million reads each.