GSM5942508: shCtrl, Jurkat, CTCF, CUT_Tag, Rep 3; Homo sapiens; OTHER
CUT_Tag experiments was performed using the Hyperactive In-Situ ChIP Library Prep Kit for Illumina (Vazyme, TD901-01). Cells were incubated with pG-Tn5 and DNA were digested 1 h at 37 ℃. Sample DNA was extracted by phenol-chloroform-isoamyl alcohol and amplified by PCR followed by sequencing.