GSM5828375: Rassf531_PBS; Drosophila melanogaster; RNA-Seq
Total RNA from mouse livers was isolated using TRIzol™ Plus RNA Purification Kit (Cat# 12183555, Thermo Fisher) and DNA was removed by treatment with TURBO DNA-free™ Kit (Cat# AM1907, Thermo Fisher). The quality of samples was determined on the Agilent Tapestation 4200, and only high quality RNA is used (RIN Score 8 or higher). The Qubit® 4.0 Fluorimeter from ThermoFisher was used to determine the concentration prior to staring library preparation. One microgram of total DNAse treated RNA was then prepared with the TruSeq Stranded Total RNA LT Sample Prep Kit from Illumina. Total RNA was depleted of its rRNA and fragmented before strand specific cDNA synthesis. cDNAs were then a-tailed and indexed adapters were ligated. After adapter ligation, samples were PCR amplified and purified with AmpureXP beads, then validated again on the Agilent Tapestation 4200.