GSM5124233: Day 07; Mus musculus; RNA-Seq
Tissue was enzymatically digested in 0.25% trypsin-EDTA for 20 min, cleaned of dead cells using the EasySep Live/Dead Annexin V magnetic selection kit, and passed through a 40-µm filter to obtain a single-cell suspension. Single-cell 3' libraries were generated using the 10X Chromium platform. Paired-end sequencing with index reads was performed on 2 lanes of a HiSeq 4000.