GSM6068660: Replicate 2: HTO-derived cDNA; Mus musculus; OTHER
Mouse tumors were digested into single-cell suspensions using the Mouse Tumor Dissociation Kit and gentleMACS dissociator (Miltenyi). CD45+ cells were enriched from each sample with magnetic beads. Enriched samples were individually hashtagged with a unique oligonucleotide barcodes (TotalSeq-A, Biolegend) and then pooled together in equal proportions at a concentration of 1000 cells/uL. Single cells were partitioned for RNA capture using 10X Chromium Controller. mRNA and TotalSeq HTO libraries were prepared with the 10X Genomics Single Cell 3' Gene Expression kit v3 using standard manufacturer protocols, pooled together (5% for HTO library), and sequenced on the NovaSeq 6000 as 400M paired-end reads.