GSM6068867: NA_KCl_2h_rep2_RNA_BS_seq; Mus musculus; OTHER
Total RNA was extracted from the neuronal cultures using TRIzol reagent combined with RNeasy min kit (QIAGEN) with DNase I on-column digestion. After two rounds of poly(A) selection, bisulfite conversion was performed and RNA-seq libraries were prepared for sequencing using standard Illumina protocols