GSM5012285: M2_ID2_KD_Rep2_bATACseq; Homo sapiens; ATAC-seq
Omni ATAC protocol(Corces et al., 2017) as previously described. Approximately 15,000 HL-60, macrophages and activated macrophages were collected for scRNA-seq. Briefly, cellular viability was assessed and cells were subsequently washed with PBS prior to library preparation. scRNA-seq protocol was performed as previously described Illumina 2016). Cells were lifted using trypsin. Total RNA was extracted by using the RNeasy kit (QIAGEN).