GSM5984918: WT-biol-rep1__RNAseq (control); Mus musculus; RNA-Seq
Pieces of organ tissue were snap-frozen in liquid nitrogen; microdissected kidney cortex pieces were stored in RNAlater; Total RNA was extracted from four kidney cortices of four mice from each of the four cohorts using RNeasy spin columns (#74134, Qiagen). All samples passed the RNA quality control that was performed with a Bioanalyzer 2100 prior to further processing. The complete transcriptomes were sequenced using an Illumina HiSeq2000 Sequencer with 51bp single-end reads and 4 samples per lane as previously described RNA libraries were prepared for sequencing using standard Illumina protocols