GSM5942352: RNA_AGER007; Homo sapiens; RNA-Seq
15.000 to 200.000 CD14hi monocytes from 11 healthy controls, 13 acute and 20 convalescent patients were isolated by FACS directly in TRIzol reagent (ThermoFisher scientific). After chloroform extraction, RNA isolation was performed using a RNeasy kit (Qiagen) and sample quality was tested on a Fragment Analyzer (Agilent) Indexed cDNA libraries were obtained  using the Ovation Solo RNA-Seq System (Tecan) following manufacturer's recommendations