GSM5861463: A1552 (#1) replicate 1; Vibrio cholerae; RNA-Seq
RNA was isolated using standard RNA isolation kits. The RNA was cleaned up after DNAse treatment using a RNA clean-up procedure (GenElute Mammalian Total RNA miniprep kit; Sigma). Next, Illumina RNA libraries incl. bacterial Ribo-depletion were prepared. RNA libraries were prepared with a TruSeq Stranded mRNA sample prep kit (Illumina).