GSM5057918: T24_CFZ_0.73_B; Mycobacterium tuberculosis; RNA-Seq
In three biological replicates, M. tuberculosis cultures with or without drug treatment were harvested at 4, 24  or 72 h. Cells pellets were spun down and immediately frozen in liquid nitrogen and stored at -80 degrees C until RNA isolation. Cell pellets were transferred to a tube containing Lysing Matrix B, and vigorously shaken at max speed for 30 s in a FastPrep 120 homogenizer three times. This mixture was centrifuged at max speed for 1 min and the supernatant was transferred to a fresh tube. RNA was isolated using phenol-chloroform extraction method, followed by DNase treatment. RNA libraries were prepared for sequencing using standard Illumina protocols