GSM5123369: S10597: Epidermal sheet-2; Mus musculus; RNA-Seq
Mouse ear was dissected and separate the dorsal and ventral side of skin. Ear was incubated with dispase solution or exofoliative toxin A solution at 37 °C. Epidermal sheet and ETA sheet were removed and subjected to isolation of total RNA. Total RNA was prepared using the RNeasy mini kit (QIAGEN). ど RNA-seq library was prepared by the NEB Next Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA). Libraries were quantified using the KAPA Library Quantification Kit (Illumina GA). Samples were multiplexed, adjusted to 2 nM, and sequenced on an Illumina HiSeq 2000 instrument. Reads were generated via 25 bp paired-end sequencing.