GSM5123821: MLKL_KO_IR_rep1; Mus musculus; RNA-Seq
Total RNA from each sample was extracted using Trizol Reagent (ThermoFisher Scientific) following standard protocols. A total amount of 2 μg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample. RNA libraries were prepared for sequencing using standard Illumina protocols