GSM5322091: VV Veh 2; Mus musculus; RNA-Seq
After rapid decapitation, brains were immediately dissected to separate the ventral hippocampus, which was rapidly flash frozen and stored at -80 °C. RNA was extracted from the ventral hippocampal tissue using Qiagen Lipid Tissue Mini Kit. A total of  at least 200ng of RNA per replicate was prepared for sequencing by The Rockefeller University Genomics Core Facility using the Tru-Seq Stranded RNA-Seq library preparation kit (Illumina, USA). Three biological replicates were used per experimental group, comprising of RNA pooled from 2 animals each. cDNA libraries were sequenced on an Illumina NextSeq 500 in a single lane to obtain 75-bp single-end reads at an approximate sequencing depth of 35-40 million reads per sample.