Metagenome of panchagavya
The concentration of 1 g of DNA was usedto prepare the whole genome library (Library Prep Kitfor Illumina (New EnglandBioLabs Inc.). Using Covaris, 151bp size was fragmented and end-repaired, immediately ligated with the adaptor, and then, the library was enriched with ten cycles of PCR. Using AgencourtAMPure XP beads (Beckman Coulter), purification was performed following manufactures instruction, and their quality was checked with Agilent 4200 TapeStation System (Agilent Technologies Inc.) before sequencing using in Illumina NextSeq 500.
PGMET_NS