RNA-seq of Sha x Lag2-2 hybrid: late outgrowth
Library preparation and sequencing was performed by BGI. In summary, mRNA was enriched following reverse transcription to double-strand cDNA. After end repair and 3'adenylation, the adaptors were ligated. The ligation products were purified and the fragments were enriched by PCR. The PCR product was heat-denatured and the single strand DNA was cyclized by splint oligo and DNA ligase, following sequencing on BGISEQ-500 platform.
l_out_2