GSM5739214: E12 het G rep 5; Mus musculus; RNA-Seq
GSM5739214
After cell sorting of E13/E16 and P0 cells, samples were incubated for 30 min at 37 °C and stored at -80°C until further usage. After thawing samples on ice, their total volume was adjusted to 250 µl using RNase-free H2O (Thermo Fisher Scientific) followed by addition of 750 µl Trizol LS (Thermo Fisher Scientific) and inverting five times. After incubating for 5-min at RT, the entire solution was transferred into a MaXtract tube (Qiagen). 200µl microliters chloroform (Sigma-Aldrich) were added. After three times 5sec vortexing and 2min incubation at RT, samples were centrifuged for 2 min with 12,000 