GSM5013449: S68 Sample1 [S1]; Stropharia rugosoannulata; RNA-Seq
Total RNA  was extracted using Plant RNA kit (OMEGA), following the manufacturer's instructions. In order to select cDNA fragments of preferentially 150~200 bp in length, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. At last, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system.