GSM4790110: Shh_GFPPositive_E10.5Head_ATAC-seq; Mus musculus; ATAC-seq
Entire E10.5 day heads were dissected from an individual Shh GFP positive embryo. A single cell suspension was made by incubating the dissected head in a 1:5 trypsin:versene solution for 15 minutes. Cells were pelleted, washed in PBS and resuspended in PBS then sent for FAC sorting for GFP positive cells. Resulting GFP positive cells were subject to the ATAC-seq protocol from Buenrostro et al. 2015. Following this protocol the samples was subject to an additional left and right size selection purification step using SPRI select beads (#B23318) at a 0.95x and 1.4x bead ratio.