MinIon sequencing of In(1)wm4 genomic DNA
DNA from In(1)wm4 flies was extracted using proteinase K treatment  phenol-chloroform extraction  ethanol precipitation protocol. The sequencing library was prepared from 1.5 g of DNA from homozygous In(1)wm4 flies according to the protocol recommended for Ligation Sequencing Kit (SQK-LSK109) and Native Barcoding Expansion 1-12 (PCR-free) from ONT. A set of .fast5 files from the sequencing run was basecalled and demultiplexed on a standalone GPU-enabled server with Guppy  Version 3.5.2 using dna_r9.4.1_450bps_hac profile.
P2A4WM_300120