RNA-Seq of mus musculus:male kidney
For animal experiments, a sample size of 5 was chosen for each experimental group. Transgenic mice were established. RNA-seq and biological assays were applied in 3 independent 6-month-old wildtype (WT) and podo-B7-1-Tg(Tg) mice to figure out the mechanism of B7-1. The proteinuria models, including ADR and 5/6NX, were conducted on the basis of our previous studies, and no outliers were removed. In vitro, we carried out RNA-seq in cultured podocyte lines to search potential pathways for B7-1 regulation.
WT2