Canine_MUO_CSF_2
RNA was extracted with TRIzol (Thermo Fischer Scientific) and reverse transcribed to cDNA with non-ribosomal hexamers using Superscript IV (Thermo Fischer Scientific) using non-ribosomal hexamers. Second-strand cDNA synthesis and random amplification of samples was performed as previously described. A DNA library was constructed using the NexteraXT protocol (Illumina) with NGS performed on an Illumina MiSeq system using MiSeq Reagent kit V3 (300 x 2 cycles; Illumina).
NGS_16002