GSM5672725: E16.5 Sox9+ Progenitor Cells - Sample 2; Mus musculus; RNA-Seq
A single cell suspension was generated from embryonic lungs isolated from Tg(Sox9-EGFP)EB209Gsat reporter mice at either E11.5 or E16.5. SOX9+ lung epithelial cells were isolated by sorting for GFP+/CD326+/CD31-/CD45-/7-AAD- using a BD/FACSAria II cell sorter. RNA was isolatedusing the RNEasy Micro Plus kit, after completing a trizol/chloroform extraction. SMART-Seq v4 Ultra-Low Input RNA kits (TaKaRa Bio) were used to generated polyA selected libraries from total RNA.