GSM5733426: P1 relapsed bladder cancer; Homo sapiens; RNA-Seq
The fresh tissue samples were washed by using Hanks Balanced Salt Solution (HBSS), and then cut them into 1~2 mm pieces. The small tissue pieces were transferred to a 15 ml centrifuge tube, and digested using XSCOPETM Tissue Dissociation Solution (Singleron) under shaking conditions at 37°C for 15 minutes. After the digestion, the samples were filtered with 40um sterile strainers, and then centrifuged at 1000 rpm at 4°C for 5 minutes. The supernatant was removed, and the cells were resuspended with 1ml PBS, then added 2 mL GEXSCOPETM red blood cell lysis buffer (Singleron) and incubated for 10 minutes to lyse the red blood cells. After single-cell suspensions with 1×10^5 cells/mL in concentration in PBS (HyClone) were prepared. Single-cell suspensions were then loaded onto microfluidic devices and scRNA-seq libraries were constructed according to Singleron GEXSCOPER protocol by GEXSCOPER Single-Cell RNA Library Kit (Singleron Biotechnologies).