GSM4787218: BM110 Wild type replicate A; Streptococcus agalactiae; RNA-Seq
Total RNA (FastRNA ProBlue, MP Biomedicals) was extracted  and residual DNA removed with the TURBO DNase (Ambion). DNAse treated RNA molecules were treated for library preparation using the Truseq Stranded mRNA sample preparation kit (Illumina, San Diego, California) according to manufacturer's instructions. The ribosomal RNA depletion was realized using the Ribo-Zero rRNA removal kit (bacteria, # MRZB12424, Illlumina). The obtained oriented libraries were controlled on Bioanalyzer DNA1000 Chips (Agilent, # 5067-1504) and quantified by spectrofluorimetry (Quant-iT™ High-Sensitivity DNA Assay Kit, #Q33120, Invitrogen).  Sequencing was performed on the Illumina Hiseq2500 platform to generate single-end 65 bp reads bearing strand specificity.