GSM4785590: IVF_F_984.1; Mus musculus; RNA-Seq
RNA were isolated from one-quarter of snap frozen liver by using phenol-chloroform extraction and TRIzol (Invitrogen, CA, USA) Total RNA (4 µg) was used as input for poly-A selection and mRNA-sequencing library synthesis using KAPA mRNA-Seq library synthesis kit and KAPA Single-Indexed adapter kit (Kapa Biosystems, Wilmington, MA, USA). Library quality control was conducted using Agilent 2100 Bioanalyzer system, High Sensitivity dsDNA Qubit system and NEBNext Library Quantification Kit (New England Biolab, Ipswich, MA, USA). Sequencing was performed on Nextseq 500 platform (Illumina, San Diego, CA).