GSM5672513: D273T4: DC_MyD88WT_TrifWT_LPS_4h_cohort1; Mus musculus; RNA-Seq
GSM5672513
RNA was first precipitated with Trizol according to the manufacturer's instructions, washed with ethanol and the pellet air-dried before in-solution DNAse (Quiagen) treatment for 30 minutes at room tempurature. DNAse-treated RNA was then column-purified using the RNA Mini kit from Quiagen and eluted in 30µl of nuclease-free H2O. PolyA+ RNA libraries were prepared using the TruSeq Stranded mRNA kit from Illumina