GSM5013522: Plate1-m38-RNA; Mus musculus; RNA-Seq
Lung and heart tissue samples were collected immediately 4 h and 24 h postexposure  and stored at -80 degrees C until further analysis.  RNA molecules with 18 nucleotides or greater were extracted with the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen) and quantified with the NanoDrop 1000 Spectrophotometer (Thermo Scientific, Waltham, Ma) and tested for quality based on RNA integrity scores produced by the QIiAxcel system (Qiagen). Isolated RNA samples were analyzed using next generation sequencing technologies enabled through the BioSpyder TempO-Seq platform. mRNA-seq libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit for Illumina, and then these libraries were pooled and sequenced (single-end 50 bp) on the Illumina Ultra-High-Throughput Sequencing System (Hiseq) 2500.