GSM5671091: V1: LNCaP Vehicle Rep1; Homo sapiens; ChIP-Seq
GSM5671091
After 4h hours, cells were fixed with formaldehyde. Nuclear pellets were sonicated on ice for 10 cycles (30 sec on, 30 sec off) using a Diagenode Bioruptor. Lysates were immunoprecipitated with 4 ug AR antibody which had been pre-coupled to Protein G Dynabeads (Life Technologies, 10004D). DNA was purified using a PCR purification kit (Qiagen) and eluted in 100 µl. Libraries were prepared according to Illumina's instructions accompanying the TruSeq ChIP Library Prep Kit