GSM5735485: Sara_Hyx_BPD; Mus musculus; RNA-Seq
Digestion of the P45 lungs with collagenase and FACS using CD31, CD45, Epcam and Sca1 The cell suspensions were counted with Biorad cell counter and diluted according to manufacturer's protocol to obtain 9000 single cell data points per sample. Each sample was run separately on a lane in Chromium controller with Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (10xGenomics). Single cell RNAseq library preparation was done using standard protocol.