16S amplicon sequeincing of soil bacterial communities
For 16S rRNA sequencing, we targeted the variable 3-4 (V3-V4) region (464 base pairs) of the 16S rRNA gene using the following forward and reverse primers: 341-F(5-CCTACGGGNGGCWGCAG-3) and 801-R (5-GACTACHVGGGTATCTAATCC-3) (Klindworth et al.,2013). Libraries were sequenced using an Illumina MiSeq Reagent Kit v3 (2x300bp paired end) on an Illumina MiSeq,
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