GSM4905070: IPA-SNC1; Mus musculus; RNA-Seq
RNAseq: RNA was extracted using RNeasy mini kit (Qiagen), according to manufacturer's guidelines. Residual DNA contamination was removed by treating the spin column with 40 units of RNase-free DNase I (Qiagen) for 15 min at room temperature prior to RNA elution. RNA concentrations and purity were verified for each sample following elution with Agilent 2100 Bioanalyzer (Agilent). RNA with RIN factor above 8.0 was used for library preparation. Libraries were generated using the NEBNext poly(A) mRNA magnetic isolation module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina according to the recommended manufacturer protocol.  Ligation and library integrity was verified using a Tapestation and Glowmax station. The NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) adapter sequences were ligated to each sample in each group to allow for sequencing multiplexing. Sample libraries ligated with unique adapter sequences, were multiplexed, and sequencing was performed in an Illumina HiSeq4000, generating 75bp pair-ended reads.