sv1000_unsw
High molecular weight gDNA (1 g) was re-extracted from the sample for long read ONT sequencing from the same brain tissue, using the 1D genomic DNA by ligation kit (SQK-LSK109, ONT) according to the standard protocol (with the inclusion of long fragment buffer). 500 ng of DNA was loaded onto two r9.4 minion flow cells and sequenced on an ONT MinION. Raw reads were converted to FASTA format, and filtered (guppy_basecaller parameters: min_qscore 7, filtlong parameters: min_mean_q 93, min_length 3000) with GUPPY (v.3.2.1) using the high-accuracy flip-flop model (config file: dna_r9.4.1_450bps_hac.cfg).
SV01_np4