RNA-seq of Oryza sativa (Wild type): Coleoptile grown under 28C and 1 mol m-2 s-1 of continuous white light.  (biological replicate 3)
Intact coleoptiles were collected from three biological replicates for each combination of temperature and light intensity, and were immediately frozen in liquid nitrogen. Total RNA was isolated from 50 mg of frozen coleoptiles with the RNeasy Plant Mini Kit (Qiagen), following the manufacturers instructions. The quantity, quality and integrity of the RNA was measured with Fragment Analyser (Agilent), using the High Sensitivity RNA Analysis, to assure that high quality RNA was used for subsequent RNA-seq
281C