GSM5706929: MFA129 D1 WT IP batch 2 [113_04940AAB_TTAGGC]; Mus musculus; RIP-Seq
RNA was extracted by phenol-chlorofom following manufacturers instructions. a single mRNA-purification step from 0.8 µg of RNA was applied to input samples using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs), and no purification steps was done on the IP samples. NGS libraries for RNA-seq were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs). Twelve and seven cycles of PCR amplification were applied to input and IP libraries, respectively