GSM5706750: IBD3552_I; Homo sapiens; RNA-Seq
Fresh or Frozen colonic biopsies from UC_I, UC_NI, CC_I, CC_NI and HC were digested to yield high viability single cells, which were put through flow-cytometry (FACS) sorting to enrich for CD45+ immune cells, before loading on to the 10X platform for single-cell RNA sequencing Samples were loaded onto the 10X sc-RNA seq platform, with GEM generation and RNA RT, followed by generation of Gene expression (GEX) libraries, as per protocol CG000183, Rev A