biological repeat 1 end 1 of input
The cDNA libraries used the Illumina ScriptSeq v2 RNA-Seq Library Preparation Kit(Epicentre).The cDNAs were purified and amplified and PCR products corresponding to 200-500 bps were purified, quantified and stored at -80 C until used for sequencing.For high-throughput sequencing, the libraries were prepared following the manufacturer's instructions and applied to Illumina HiSeq X Ten system for 150 nt paired-end sequencing by ABlife. Inc (Wuhan, China).
PTRF_input_1-mRNA_end1