GSM5939574: Sagittal plane of orchid flowers (buds 1-6); Phalaenopsis aphrodite; RNA-Seq
GSM5939574
Dissections were performed and Phalaenopsis Big Chili flowers at early developing stages were immediately frozen in liquid nitrogen-cooled isopentane and were embedded in pre-cooled OCT. Tissue preparation and optimization were performed on 10X Visium platform, including tissue sectioning, staining, tissue permeabilization, fluorescent cDNA synthesis and imaging, according to the manufacturer's protocol. cDNAs of samples were sequenced on Illumina novaseq platform and were subjected to 150 cycles of paired-end (2 × 150 bp) sequencing