GSM6212698: Experiment #1 Test Tube Fresh BG-11 Biofilmers; Synechococcus elongatus PCC 7942 = FACHB-805; OTHER
GSM6212698
Frozen cell pellets were thawed and genomic DNA was extracted using a phenol-chloroform and ethanol precipitation protocol, as previously described by Clerico, et al 2007. Barcodes of transposon insertions in the pooled library were PCR amplified. Primers for this amplification contained adapters for Illumina sequencing, experiment-specific barcodes, and heterogenous spacers for enhanced length diversity, as described by Wetmore, et al. (2015). Amplicons were assessed by gel electrophoresis and purified using a PCR-purification kit (Zymo).