GSM5236018: Normal_secondtrimester_3; Homo sapiens; Bisulfite-Seq
Genomic DNA was extracted from frozen samples using Genomic Tip-500 columns (Qiangen, Valencia, CA, USA) and from bisulfite-converted samples using an EZ DNA Methylation™-GOLD Kit (Zymo Research, CA, USA) according to the manufacturer's instructions. Libraries from different samples were quantified and pooled together, followed by sequencing on the Illumina HiSeq platform according to manufacturer's protocols. Sequencing was performed with a 2x150bp paired-end mode. Targeted Bisulfite-Seq