16S library of coastal seawater - 24 Jul 2012 (weekly sample)
We extracted DNA using phenol-chloroform chemical extraction (Massana et al., 1997) supplemented with three rounds of bead beating (30 seconds each). Microbial communities were characterized using a dual index approach with the following portions of the primer targeting the V3/V4 region of the bacterial and archaeal 16S rRNA gene: S-D-Bact-0341-b-S-17 (16S F V3; CCTACGGGNGGCWSCAG) and Arch806 (16S R V4; GGACTACNVGGGTWTCTAAT).
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