GSM4787167: AK010F: A549 wt, 1 µM trimer 2-5A, 4.5 h; Homo sapiens; OTHER
Ribosome profiling lysates were clarified, Rnase I treated, and separated over sucrose cushion. RNA was then extracted using trizol and footprints of the specified size were retained by gel purification. Samples starting with "GJ" were prepared accoring to Ingolia et al, 2009. Samples starting with "AK" were prepared using the protocol published in McGlincy and Ingolia, 2017. Briefly, RNAs were ligated to a universal DNA linker followed by reverse transcription. rRNA was subtracted using Ribo-Zero Illumina kit after ligation. cDNAs were then circularized and PCR amplified.