GSM5129870: IL-4Rα-/- 3; Mus musculus; RNA-Seq
RNA was isolated from spleen monocytes using Maxwell® RSC simplyRNA Tissue Kit (Promega, AS1340) Sequencing libraries were be prepared using the NEBNext Poly(A) mRNA Magnetic Isolation Module and the NEBNext UltraTM II Directional RNA Library Prep Kit for Illumina according to manufacturer's protocols (New England Biolabs). Libraries were QC-checked on a Bioanalyzer 2100 (Agilent) using a High Sensitivity DNA Kit for correct insert size and quantified using Qubit dsDNA HS Assay (Invitrogen).