GSM5627682: Non-treated H1299; Homo sapiens; RNA-Seq
Total RNA was prepared from cell lines using miRNeasy RNA isolation kit (Qiagen, Inc.). Firstly, ribosomal RNA was removed by Epicentre Ribo-zeroTM rRNA Removal Kit (Epicentre, USA), and rRNA free residue was cleaned up by ethanol precipitation. Subsequently, sequencing libraries were generated using the TruSeq Stranded Total RNA LT Sample Prep Kit (Gold) for Illumina® (NEB, USA) following manufacturer's recommendations.