GSM5739998: RNA-seq of MCF-7, untreated, Replicate #2; Homo sapiens; RNA-Seq
GSM5739998
Total RNAs were isolated using E.Z.N.A.® MicroElute Total RNA Kit (Omega), and DNA was removed by on-membrane DNase I (Omega) digestion according to the manufacturer's protocol. Then, isolated RNA integrity and quality were determined by Bioanalyzer (Agilent 2100) by using the Agilent RNA 6000 Nano kit. 500 ng of total RNA was used as the input for mRNA capture with oligo-dT coated magnetic beads. The library was prepared using Illumina TruSeq Stranded mRNA Library kit according to manufactured protocol and final purified products were then quantitated by qPCR before cluster generation and sequencing. Then, samples were sequenced 2x 76 cycles run on NextSeq500 sequencer. The samples have 21 to 56 million pass filter reads with more than 94% of bases above the quality score of Q30.