GSM4785587: f82199; Mus musculus; RNA-Seq
epidermal cells were isolated accoring  to Ghouse et al., Cell Rep. 2018 Jan 2;22(1):27-35. doi: 10.1016/j.celrep.2017.12.010. CD45- CD49f+ cells were FACS-sorted for RNA extraction. Polyadenylated RNA was reverse transcribed and amplified with 13 cycles using the SMARTseq2 protocol, before subjecting 3 ng of the cDNA to a tagmentation based library preparation (TruePrep DNA Library Prep Kit V2 for Illumina, Vazyme) followed by sequencing on a Nextseq500 to sequencing depth of 30 Mio. reads.