GSM4969943: Lint-O-ChIP-seq rep 2; Drosophila melanogaster; ChIP-Seq
Lysates were clarified from sonicated nuclei and DNA-protein complexes were immunoprecipitated with antibody Libraries were prepared according to illumina's instructions accompanying NEBNext Ultra 2 FS DNA Library Prep Kit (E7805). In the adaptor ligation step, the original adaptor reagent was diluted to 10 times. After adapter ligation DNA was purified with AMPure XP beads(Beckman Coulter) for size selection. Then DNA was PCR amplified with Illumina primers for 13~16 cycles and library fragments of ~325 bp (insert plus adaptor and PCR primer sequences) were purified with AMPure XP beads(Beckman Coulter). The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Illumina HiSeq following the manufacturer's protocols.