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The experiment was conducted during the 2020 growing season (June–October) at the experimental station of the Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China (116.34′ E, 39.97′ N). Plants were grown in a climate chamber under controlled conditions: 28°C, 70% relative humidity, and a light intensity of 165 μmol m⁻² s⁻¹. Genomic DNA was extracted from young leaf tissues (14-day-old) of 20 wild, 20 landrace, and 20 cultivated accessions using the cetyltrimethylammonium bromide (CTAB) method, followed by bisulfite conversion. Sequencing libraries were prepared with the EpiArt DNA Methylation Library Kit for Illumina V3 (NE103) and sequenced on an Illumina NextSeq 500 platform.
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