GSM5736081: Gli1_TAC_28; Mus musculus; RNA-Seq
Hearts were harvested 28 days after surgery and single cell solution was generated by enzymatic digestion (30min, 37°C) and mechanic dissociation (C-tube, gentleMACS). Tdtomato positive, DAPI negative cells were sorted and pooled from 3-5 mice per condition and genotype. Single cell solutions were then loaded on a Chromium Single Cell B Chip Kit following the manufacturer protocol. All protocols to generate scRNA-seq data on the 10x Chromium platform, including sample prep, library prep, instrument and sequencing settings, are available here:https://support.10xgenomics.com/single-cell-gene-expression Single Cell 3' v3.1