RAD-Seq of Camassia and Hastingsia for phylogenetic analyses
Genomic libraries were generated using the modified single-digest RADseq method known as multiplexed shotgun genotyping (Andolfatto et al., 2011; Baird et al., 2008). DNA from each individual was digested with restriction enzyme AseI (NEB Biolabs), unique 6-bp bar-coded adapters were ligated to each sample, and the libraries were size-selected for fragments of 275 bp using a Pippin Prep (Sage Science). Products from 14 cycles of PCR were purified with AMPure beads (Agencourt) and sequenced as pools.
Qb_BOG_1M