GSM6069121: AZA.rep2; Mus musculus; RNA-Seq
GSM6069121
1 – 5 x 10^5 I1DN progenitor cells (cKit+CD11b-) per mouse were isolated by FACS, washed with PBS and pelleted by centrifugation (450 x g, 10 min, 4 oC) prior to being stored at -80 °C in TRIzol Reagent (Invitrogen). RNA was extracted with the Direct-zol RNA Microprep kit as per manufacturer's instructions (Zymo Research). Purified RNA was quantified using the TapeStation 2200 system (Aligent) with RNA HS screentape (Aligent) according to the manufacturer's instructions. The Molecular Genomics Core of the Peter MacCallum Cancer Centre used the QuantSeq 3´ mRNA-seq Library Prep Kit for Illumina (Lexogen), according to the manufacturer's instructions