Virome analysis reveals the wide viral spectrum of Growth Retardation Disease and normal Macrobrachium rosenbergii in China
The gill, hepatopancreases, and muscle tissues were homogenized, frozen, and thawed three times. The suspension of each pool was subjected to centrifugation, and the supernatant was filtered through a 0.45 m filter. RNA was extracted using the QiaAmp Mini Viral RNA kit and quantified using Qubit 4.0. The ribosomal RNA was removed by the NEBNext rRNA Depletion Kit . Sequencing libraries were generated by using RNA Library Prep Kit for Illumina(NEB, USA). Then, the 150 bp paired-end sequencing of the RNA libraries was performed using the Illumina NovaSeq platform.
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