GSM4785616: Tumor Treg pool 4; Mus musculus; RNA-Seq
RNA was isolated using the RNeasy Mini Kit (Qiagen) and processed as per manufacturer's instructions. cDNA synthesis and amplification was performed using the SMART-Seq Ultra® v4 Ultra® kit (TakaRa Bio USA, Inc.). The cDNA amplicons were quantified using Qubit (1X dsDNA High Sensitivity Assay) and analyzed with Agilent 2100 Bioanalyzer (High Sensitivity DNA Kit) for quality control. Sequencing libraries were constructed using the Nextera XT DNA Library Preparation Kit (Illumina, Inc.) from the cDNA.