GSM5014063: Chronic-1-scRNA; Mus musculus; RNA-Seq
The mucosal layer was torn from mice bladder and dissociated immediately. For scRNA-seq, the tissues were dissociated into single cells suspensions using digestion buffer (DMEM/F12 Medium+ 2 mg/ml of collagenase IV + 100 IU/ml DNase I), and single cell GEMs were generated by 10X genomics Chromium Controller. cDNA library was constructed following the manufacture's protocol.