GSM5592809: JUNB-overexpressing_after_BMP_ActA_RNASeq [PREP0095_RHuan11781A_D02v1_12_RH_1028_S4]; Homo sapiens; RNA-Seq
Total RNA was prepared from Accutase-dissociated cells with an RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. RNA integrity was quantified with a TapeStation 4200 (Agilent). All RINe scores were ≥ 9.9. Libraries were constructed from total RNA using a Kapa mRNA HyperPrep Kit (Roche) by the Bauer Core at Harvard University.