GSM4787289: NbrCTD overexpression in S2 nbrKO; Drosophila melanogaster; miRNA-Seq
Total RNA was extracted from S2 cell culture collected in Trizol using a phenol-chloroform-based standard protocol, according to manufacturer's instructions. Small RNA sequencing libraries were constructed from an input of 25ug total RNA. In-house protocol: size select 18-30nt RNA; deplete 2S rRNA; ligate 3p linker; size select ligation product; ligate 5p linker; size select ligation product; reverse transcription; qPCR library amplification. 3p and 5p adapters contain 4N at ligation interface (Jayaprakash et al, NAR, 2011)