WGS of Cyrtorhinus sp.: adult individuals
After DNA extraction, 1 g genomic DNA was randomly fragmented by Covaris, followed bypurification by AxyPrep Mag PCR clean up kit. The fragments were end repaired by End RepairMix and purified afterwards. The repaired DNAs were combined with A-Tailing Mix, then theIllumina adaptors were ligated to the Adenylate 3Ends DNA and followed by products purification.The products were selected based on the insert size. Several rounds of PCR amplification with PCRPrimer Cocktail and PCR Master Mix were performed to enrich the Adapter-ligated DNA fragments.After purification, the library was qualified by the Agilent Technologies 2110 bioanalyzer and ABIStepOnePlus Realtime PCR System. Finally, the qualified libraries were sequenced pair-end using HiSeq X Ten.
BL_036