GSM5235644: Acute population: Day 2 (“acute” CCNE1 overexpression, 48h) [t1_3]; Homo sapiens; RNA-Seq
Lysates were generated and RNA was extracted from cells using Quick-RNA Miniprep Kit (Zymo Research, Irvine, CA, USA) with chloroform extraction. RNA was quantified with the Qubit RNA HS Assay Kit (Invitrogen, Carlsbad, CA, USA) Libraries for each sample were prepared, at the same time, using KAPA Hyperprep mRNA Library Kit (Roche) and Illumina adapters (New England Biolabs) starting with 1 µg RNA for each sample.