Paulownia fortunei
Chromatin was fixed in place in the nucleus using formaldehyde and then extracted. Fixed chromatin was digested with DpnII, the 5' overhangs were filled using biotinylated nucleotides, and free blunt ends were ligated. After ligation, crosslinks were reversed, and DNA was separated from protein. Purified DNA was treated to remove biotin outside of the ligated fragments, sheared to a mean fragment size of 350 bp, and used to create sequence libraries with NEB Next Ultra enzymes (New England Biolabs, Ipswich, MA, USA) and Illumina-compatible adapters. Biotin-containing fragments were isolated using streptavidin beads before PCR enrichment of the libraries; the libraries were next sequenced on the Illumina HiSeq PE150 platform.
FDHC202123088-1a