GSM5600380: Library_285_S2; Mus musculus; RNA-Seq
GSM5600380
Microdissected E9.5 hearts and the pharyngeal mesoderm immediately posterior to the outflow tract and anterior to the neural tube were isolated into Trizol and RNA was obtained using Qiagen RNeasy spin columns, with on-column DNAse I digestion following manufacturer's directions. Bulk RNA libraries were prepared using the NEBNext Ultra II DNA Library Prep kit (NEB) with the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) to enrich poly-A tailed RNA molecules. Libraries were PCR'ed with single end barcodes using Illumina Multiplex Oligos (NEB).