RNA-Seq of Myxocyprinus asiaticus: male muslce
RNA was extracted with TRIzol Reagent (Invitrogen). The quality of RNA was checked with A Nanodrop ND1000 spectrophotometer (LabTech) and a 2100 Bioanalyzer (Agilent Technologies). The mRNA was reverse transcribed using a Clontech  SMARTer cDNA synthesis kit. The libraries were constructed according to protocols suggested by the manufacturer, and then Illumina NovaSeq6000 platform was used for sequencing with 150 bp paired end reads.
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