GSM5013866: V5 GSE1 DMSO ChIP; Homo sapiens; ChIP-Seq
Cells were lysed with SDS buffer and chromatin was subjected to 35 cycles of sonication prior to the incubation with the anti-V5 antibody. DNA libraries were prepared with 10 ng of DNA through an in-house protocol (modified from Blecher-Gonen et al., DOI: 10.1038/nprot.2013.023) by the IEO genomic facility and sequenced on a NovaSeq 6000 (Illumina) instrument.