GSM5672477: 3-day differentiated myobundles; Homo sapiens; RNA-Seq
Single cell suspensions were loaded on the 10x Genomics Chromium Controller Single-Cell Instrument (10x Genomics) using the Chromium Single Cell 3' Reagent V3 Kit. Cells were mixed with reverse transcription reagents, gel beads, and oil to generate single-cell gel beads in emulsions (GEM) for reverse transcription (RT). After RT, GEMs were broken and the single-stranded cDNA was purified with DynaBeads. cDNA was amplified by PCR and the cDNA product was purified with the SPRIselect Reagent Kit (Beckman Coulter). Sequencing libraries were constructed using the reagents provided in the Chromium Single-Cell 3′ Library Kit following the user guide (https://support.10xgenomics.com/single-cell-gene-expression/library-prep). scRNA libraries were sequenced with the DNBseq platform by BGI Genomics.