GSM5014410: 86-week-old_RNA-seq_rep1; Mus musculus; RNA-Seq
Whole mouse aortas were harvested from 4-week-old, 26-week-old, and 86-week-old male C57/BL6J mice. The isolated aorta included the aortic arch, as well as the ascending, descending, thoracic, and abdominal portions. Perivascular fat was dissected from the vascular tissue before dissociation. Single cells were captured in droplet emulsions using a GemCode Single-Cell Instrument (10X Genomics). Nuclei were isolated from cells dissociated from the aorta based on the protocol of the Nuclei Isolation for Single-Cell ATAC-Sequencing (10X Genomics). Single-cell mRNA-sequencing and single-cell ATAC-sequencing: Libraries for single-cell mRNA-sequencing were constructed as per the 10X Genomics protocol using the Single-Cell 3' v2 Reagent Kits from 10X Genomics, whereas  single-cell ATAC-sequencing libraries were prepared using the Chromium Single-Cell ATAC Library & Gel Bead Kit (#1000110, 10X Genomics).