GSM5740243: PDX6-B16-1; Homo sapiens; RNA-Seq
GSM5740243
Primary tumor tissues from these PDX models were dissociated enzymatically/mechanically using GentleMACS Dissociator (Miltenyi Biotec), as per the manufacturer's guidelines. live cells were isolated via density gradient centrifugation in Percoll Plus (GE Healthcare Life Sciences, CC-17-5442-01). RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols