GSM4781323: space_microg_rep2; Homo sapiens; RNA-Seq
Total RNA was extracted with ReliaPrep RNA Cell Miniprep System (Promega, Madison WI, USA). RNA integrity was assessed with agarose gel electrophoresis and ethidium bromide staining/UV light (GenoPlex, VWR, USA), concentration and purity with NanoView Spec- trophotometer (GE Healthcare, Pittsburgh, PA, USA). The RNA-seq libraries were prepared using TruSeq Stranded Total RNA with Ribo-Zero Library Prep Kit.