GSM6212225: Amphimedon queenslandica, ribosomal RNA depleted RNA-Seq, B; Amphimedon queenslandica; RNA-Seq
GSM6212225
Total RNA was extracted separately from the six replicated biopsies using TRIzol (Sigma-Aldrich) and DNA was removed with Deoxyribonuclease I (Invitrogen) Three of the samples (D,E, and F) were subjected to the standard TruSeq Stranded mRNA-seq workflow, using the poly-A pulldown and standard protocol with 1 µg total RNA as input. The other three samples (A,B,and C; 2.5 µg total RNA each) were first rRNA-depleted using the Ribo-Zero Gold ribosomal RNA Removal Kit. 100 ng of the depleted RNA was then used as input to the mRNA-seq protocol, but omitting the poly-A pulldown and instead of starting at the RNA fragmentation step. 