GSM5671727: aorta postnatal 0.5 day; Mus musculus; RNA-Seq
For cell isolation, aorta and ducti were dissected in versene, vessels were minced into small pieces and placed in 1mL of digestion buffer containing DNase1, 1M HEPES, Libase, and HBSS at 37C for 20mins. Once tissue was digested into a single cell suspension, cells were washed, pelleted, and passed through a 40m filter. The final cell suspension was in 0.04% BSA. scRNA-seq libraries were generated using 10X Genomics Chromium Single Cell 3' Library & Gel Bead Kit v3. Cells were loaded accordingly following the 10X Genomics protocol with an estimated targeted cell recovery of 6000 cells.