GSM5627487: Cortical_shPum2_Rep1 [JE01003]; Rattus norvegicus; miRNA-Seq
Total RNA was batch-isolated using the miRNeasy Mini kit (Qiagen) according to manufacturer's protocols. Only RNA with an RNA integrity number (RIN) 9.5, determined on a Bioanalyzer 2100 (Agilent), was subjected to library preparation. Sequencing libraries were prepared using the QIAgen smallRNA Library Prep Kit with Unique Molecular Identifiers (UMIs) according to manufacturer's protocols.