GSM5919543: Control female; Mus musculus; RNA-Seq
GSM5919543
Kidneys were dissociated with liberase TM, hyaluronidase, DNaseI at 37C for 20 min, followed by incubation with 0.25% trypsin EDTA at 37C for 10 min. Trypsin was inactivated using 10% fetal bovine serum in PBS. Cells were then resuspended in PBS supplemented with 0.01% bovine serum albumin then filtered through 40 µm strainer and utelized for GEM generation Followed standard procedure of Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1 Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1