RNAseq of Archegozetes longisetosus: egg females
RNA was extracted by crushing eggs in a mixture of cell lysis buffer and murine RNase Inhibitor (New England Biolab) and directly preparing the library with the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina together with NEBNext Multiplex Oligos for Illumina (New England Biolab) kits for library preparation, including reverse transcription of poly(A) RNA, amplification full-length cDNA, fragmentation, ligation and final library amplification. The library was sequenced with on an Illumina HiSeq2500 platform (single-end with read lengths of 50 bp) with ~18 million reads.
Along_life_17