GSM5735500: i-SOX2-LNCap Sample 3; Homo sapiens; RNA-Seq
RNA isolation was performed using the RNeasy kit (Qiagen, Germantown, MD). Cells were lysed using RLT buffer and Qiashredder columns (Qiagen, Germantown, MD) as outlined in the manufacturer's protocols. i-SOX2-ONS76, i-SOX2-LNCaP, and i-Omomyc-ONS76 cells were cultured in the presence or absence of Dox for 4 days. mRNA was harvested as outlined above. RNA samples were assessed for purity and quality. Library preparation and transcriptome sequencing on an Illumina NovaSeq 6000 platform were performed by Novogene (Beijing, China).