WT-AsCas12a RNP targeting AP3B1 in HEK293 cells using AP3B1-80-AS guide RNA
Targeted loci were amplified with a single primer pair; subsequently, universal amplification was used to install unique index sequences. Illumina sequencing was carried out on a MiSeq using V2 chemistry and 2x150 paired-end reads. Primer sequences and protocols are described in the manuscript.
20190328-G3CBM-S52