NextSeq 500 paired end sequencing; Genome-wide distribution of Rad26 and Rad1-Rad10 in relation to Mediator and RNA polymerase II
Genome-wide distribution of Rad26 and Rad1-Rad10 in relation to Mediator and RNA polymerase II
Med17_kin28ts_p
Following a 75 minutes shift at 37°C, after reaching exponential phase at 25°C in YPD, cells were cross-linked with 1% formaldehyde during 10 minutes. DNA was extracted using the Auto iPure V2 kit from Diagenode, following the manufacturer recommendations.  Library construction was done according to the takara SMARTER thruplex (takara bio) protocol, following manufacturer's recommendation. Libraries were sized with AMPure beads (Beckman-coulter) to a final size of 325bp.