GSM5610547: Spleen TRC SpiB KO rep2; Mus musculus; RNA-Seq
Spleens were disgested with Collagenase D + Dispase II and TRCs sorted using FACS Aria III. Total RNA was extracted using Qiagen RNeasy plus microkit Library was constructed by using the Ribosomal RNA depletion using Nugen Ovation SoLo RNA-Seq system and Nugen Ovation SoLo library construction chemistry