GSM5013437: Muscle stem cells - GFP; Mus musculus; ChIP-Seq
Lysates were clarified from sonicated nuclei and DNA complexes were isolated with antibody. Library was prepared according to NEBNext Ultra DNA Library Prep Kit (E7370) by the steps of end-polishing, dA-addition and the DNA fragments with A tail were ligated to sequencing adaptors. After 100bp-500bp size selection and PCR amplification, the DNA libraries were quantified and sequenced on HiSeq2500 platform using single-end 50bp sequencing based on manufacturer's instructions.