WGS of Staphylococcus aureus
Isolate grown overnight under nonselective conditions. Cells underwent high molecular weight DNA extraction using the Qiagen DNeasy Blood & Tissue Kit (Qiagen, 69504) according to the manufacturer's instructions, with modified lysis conditions. Bacterial cells were lysed by suspending cells in 3 L of 100 mg/ml RNase A (Ambion, AM2286) and ten L of 100 mg/ml lysozyme (Sigma, L1667-1G) for 30 minutes at 37C, followed by incubation with Proteinase K for one hour at 56C and two rounds of bead beating of one min each using 01mm silica beads (MP Bio).
ER14094