Crispr-Cas9 CNBP enrichment + ONT sequencing of Homo sapiens
Genomic DNA was extracted from blood and resuspended with Tris-EDTA buffer pH 8. The crRNAs designed to bind near CNBP repat were used together with transactivation crRNA in the presence of HiFi Cas9 Nuclease for the generation of Ribonucleoprotein (RNP) complexes according to the ONT protocol. Input gDNA dephosphorylation, Cas9-mediated digestion and dA-tailing were performed based on the ONT protocol.
ONT_Crispr-Cas9_C