E. coli isolated from bloodstream infection
Genomic DNA was fragmented by tagmentation using the Nextera XT DNA library preparation kit, according to the manufacturers instructions, yielding fragments with an average size of 1kb. A genomic library was prepared using the HiSeq rapid SBS v2 library kit (Illumina USA), which was subsequently sequenced on the Illumina HiSeq 1500 platform using the 2  250-bp protocol
88_EC121