GSM5830124: ScRNA-seq of Aortic VSMCs; Mus musculus; RNA-Seq
GSM5830124
The aorta was dissected from the root (distal to the aortic valve) to the femoral artery bifurcation. Enzymatic dissociation protocol was used to separate the medial cell layer and prepare single cells. Using Single Cell 3' Library and Gel Bead Kit V3 (10x Genomics, 1000075) and Chromium Single Cell B Chip Kit (10x Genomics, 1000074), the cell suspension (300-600 living cells per microliter determined by Count Star) was loaded onto the Chromium single cell controller (10x Genomics) to generate single-cell gel beads in the emulsion according to the manufacturer's protocol. Captured cells were lysed and the released RNA were barcoded through reverse transcription in individual GEMs. The libraries were finally sequenced using an Illumina Novaseq6000 sequencer with a sequencing depth of at least 100,000 reads per cell with pair-end 150 bp (PE150) reading strategy.