GSM5129173: H2-Ab1fl_3; Mus musculus; RNA-Seq
Spleens were removed, processed and enriched for CD4+ T cells using Dynabeads™ Untouched™ Mouse CD4 Cells Kit and stained with anti-CD4, anti-CD90.1 and LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit and 12000 cells sorted on FACS Aria III equipment into extraction buffer of Arcturus PicoPure RNA isolation kit and flash frozen on dry ice, and RNA was harvested usingArcturus PicoPure RNA isolation kit with 750 pg of total RNA , for the construction of sequencing libraries. RNA libraries were prepared for sequencing using NEBNext® Single Cell/Low Input cDNA Synthesis & Amplification Module