GSM5919958: E12half_MC_ATAC_rep1; Mus musculus; ATAC-seq
E12.5 mandibular processes were dissected from wildtype embryo heads under stereomicroscope. Dissected tissues were then digested with trypsin and collagenase type II to acquire single-cell suspension for downstream library preparations. ATAC-seq was performed as described previously with TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme TD501). Briefly, 50,000 cells were collected at 500×g for 5 min and resuspended in 50μl cold ATAC-seq lysis buffer and incubated on ice for 10min. Following centrifugation at 500×g at 4°C for 5 min, cells were pelleted and resuspended in 50μl transposition mix and then incubated at 37°C. The reaction was purified with VAHTS DNA Clean Beads and PCR amplified for 15 cycles. Purified ATAC-seq libraries were sequenced on the NovaSeq platform.