GSM5918654: K562 DAC3L1; Homo sapiens; OTHER
GSM5918654
Bulk genomic DNA was extracted using the DNeasy kit (Qiagen, 69504) according to the manufacturer's recommendations. Bulk total RNA was extracted using TRIzol reagent (Invitrogen, 15596018) according to the manufacturer's recommendations. For single cell experiments, whole cell lysate was used by soring cells via FACs directly into lysis buffer. scDyad-seq is performed similar to scDyad&T-seq, except the initial reverse transcription and second strand synthesis steps are replaced with the equivalent volume of 1x NEBuffer 4. In addition, as the transcriptome is not captured, IVT is not performed and steps involving RNA enrichment and processing are omitted.