NGS of mus musculus: duodenum
DNA isolation, triplicate PCR for 16S rRNA DNA - V4 region with modified universal primers (515F-806R), amplicon pooling, quantification, library formation, 250 bp paired end sequencing with mix of library (90%) and PhiX DNA (10%) using MiSeq Reagent kit V2 (500 cycles)
806rcbc381