GSM6212899: 2671_CTRL_CAU; Homo sapiens; RNA-Seq
RNA was extracted from powdered, frozen brain tissue using standard TRIzol (Invitrogen) extraction method. RNA samples were treated with RQ1 RNase-free DNase (Promega), purified by TRIzol LS (Ambion) and checked on Agilent Bioanalyzer Polyadenylated mRNA was collected by Dynabeads mRNA Purification Kit (Ambion). The libraries were prepared by TruSeq RNA Sample Preparation Kit v2 (Illumina) following manufacturer's instructions. High-throughput sequencing was performed on NovaSeq (Illumina) to obtain 150 nucleotide paired-end reads. polyA selection, TruSeq library prep (stranded)