GSM5592867: FXS-iPS848_dCas9-gNHG3_36d_rep1; Homo sapiens; RNA-Seq
Total RNA was extracted by RNAzol and isopropanol precipitation. Poly(A) tailed mRNAs were purified by using NEBNext Poly(A) mRNA Magnetic Isolation Module (#E7490) RNAseq libraries were constructed by using NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina (#E7760S) by following the protocols from the manufacturer.