GSM5945249: CNE2 p53KO; Homo sapiens; ChIP-Seq
GSM5945249
For RNAseq, cells samples were lysed in Trizol reagent (Invitrogen, 10296010).ChIP-seq, cells were fixed, quenched, sonicated to ~250 bp in 1% SDS lysis buffer, and incubated with Protein A/G Dynabeads and 5 µg of the indicated antibody. 2% pre-cleared chromatin prior to primary antibody addition was kept as input DNA. After wash, treatment with RNAse A and Proteinase K, DNA was eluted and purified with Qiagen's PCR purification kit (28106). For RNASeq, library construction was performed using PE150 high-throughput sequencing. For ChIPSeq, library construction was performed using PE75 high-throughput sequencing.