GSM5235933: SNI 2; Rattus norvegicus; RIP-Seq
Total RNA from each sample was isolated using TRIzol reagent (Invitrogen) and then quantified using a NanoDrop ND-1000 instrument. The Arraystar Seq-StarTMpoly(A) mRNA Isolation Kit (Arraystar, MD, USA) was used to extract Poly(A) mRNA. The concentration, purity and integrity of extracted RNA .  Fragment mRNA into 100-nucleotide fragments, and then use agarose electrophoresis to determine the post-fragmentation size distribution. mRNA was divided into two samples: input and IP samples m6A RNA immunoprecipitation was performed with the GenSeqTM m6A RNA IP Kit (GenSeq Inc., China) by following the manufacturer's instructions. Both the input sample without immunoprecipitation and the m6A IP samples were used for RNA-seq library generation with NEBNext® Ultra II Directional RNA Library  Prep Kit (New England Biolabs, Inc., USA). The library quality was evaluated with BioAnalyzer 2100 system (Agilent Technologies, Inc., USA). Library sequencing was performed on an illumina NovaSeq 6000 instrument with 150bp paired-end reads.