GSM5238756: YPB-rep2; Homo sapiens; ChIP-Seq
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. The libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (cat# E7645S) from New England Biolabs Inc. (Ipswich, MA). The following steps were followed for library preparation: end repair, 5' phosphorylation, dA-tailing, adapter ligation, U excision, cleanup of adaptor-ligated DNA without size selection, and library amplification for 12 to 13 cycles followed by clean-up with Agencourt AMPure XP. The quality of the libraries was assessed using the Agilent High Sensitivity DNA kit (cat# 5067-4626) on the Agilent 2100 Bioanalyzer (Agilent Technologies) and quantified using the NEBNext Library Quant kit for Illumina (cat# E7630S) from New England Biolabs Inc