GSM6213095: scRNA_seq_Anagen; Bos grunniens; RNA-Seq
GSM6213095
Skin tissue was collected from the scapular anagen of healthy Tianzhu white yak about 2 years old. After cleaning and disinfection, the skin tissue was washed twice with PBS and 75 % ethanol, and cut into 0.5 × 0.2 cm strips. The skin tissue was digested with 0.25 % disperse enzyme in 37 ° C incubator for 2 hours, and the single hair follicle was pulled out. After digestion with 0.25 % trypsin for 0.5 hours, cells were observed under a microscope to see if they all fell off the hair shaft. After repeated blowing, the cells were filtered and centrifuged by 40 μm cell sieve, and the cells were resuspended by DMEM. Library was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics).