NextSeq 500 sequencing; RNA-Seq from flowers of wildtype Arabidopsis thaliana and DWEORG1 (At3g15590) mutant
RNA-Seq from flowers of wildtype Arabidopsis thaliana and DWEORG1 (At3g15590) mutant
RNAseq-Col-0 biol rep 1_s
Flowers were harvested simultaneously for all genotypes, snap-frozen in liquid nitrogen and stored at -80°C until use. The plants were grown in the Greenhouse under long day conditions.  Total RNA extraction in Trizol from flowers. Total RNA were treated with Dnase and depleted of rRNAs using the RiboMinusTM Plant Kit for RNA-Seq (invitrogen), following the manufacturer's instructions. The sequencing libraries of total RNA of two biological repeats per genotype were prepared using the NEXTflex Rapid Directional RNA-Seq Kit (Bioo Scientific)  following the manufacturer's instructions.