GSM5129542: 20Soy_SAA_2; Mus musculus; RNA-Seq
Flash frozen liver was dissected and a small piece was placed in Trizol. After homogenization, BCP was added, samples were vortexed vigorously, and then spun. The aqueous phase was combined with an equal volume of ethanol and the sample was processed through a Qiagen RNeasy column according to the manufacturer's instructions. mRNA was stored at -80°C and samples were diluted to 100ng/µl for RNAseq. Quant-Seq  3' mRNA-Seq Library Prep Kit indexed and pooled samples, 50bp forward read