Hi-C-seq of SiHa
Hi-C sequencing (Hi-C-seq) was performed by Annoroad Gene Technology Co., Ltd. (Beijing, China). Cells were first crosslinked and then were lysed under ice condition to the extracted DNA. After quantification, the final sequencing library was diluted to 1 ng/l. StepOnePlusTM Real-Time PCR system was used for qPCR to accurately quantify the concentration of the library. The TruSeq PE Cluster Kit v3-cbot -HS (Illumia) reagent was used to generate clusters on the cBot. After that, the library was sequenced on Illumina HiSeq X Ten platform with PE150 setting.
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