GSM5733713: WT1; Mus musculus; ATAC-seq
The Nextera DNA Library Preparation Kit (Illumina) was used to perform the transposition. 50000 nuclei were pelleted and resuspended with transposase, for 30 minutes at 37 ℃. The transposed DNA fragments were purified immediately after with a MinElute PCR Purification Kit (Qiagen). After samples were PCR-amplified using 1X NEBNext High-Fidelity PCR Master Mix (New England Biolabs, MA) libraries were purified with the MinElute PCR Purification Kit (Qiagen)