RNA-seq of Pardosa pseudoannulata mix egg-adult
Total RNA was extracted from 15 individual samples representing developmental significance ranging from egg to adults including male and female of different reproductive states with TRIzol Reagent. The total RNA samples were then pooled at equal molar ratio for Iso-Seq. RNA libraries were constructed with TruSeq RNA v2 kit and sequenced using the PacBio Sequel II platform. PacBio sequencing, RNA sequencing, and Hi-C sequencing were performed by Berry Genomics (Beijing, China).
Parps_full_length_transcriptome_2021