GSM5945667: CariesD; Homo sapiens; RNA-Seq
GSM5945667
Total RNA was isolated from the dental pulp tissues of normal, deep caries and pulpitis teeth using Trizol reagent (Invitrogen, USA). A total of 12 RNA samples (4 samples each group, 3 groups in total) were subjected to RNA sequencing. RNA libraries were prepared for sequencing using the following protocols: Briefly, the total RNA samples were processed by mRNA enrichment or rRNA removal to gain mRNA fragments. Random N6 primers were used for reverse transcription (RT) to form double-stranded DNA. Polymerase chain reaction (PCR) amplification was performed after the double-stranded DNA ends were processed. PCR products were thermally degenerated into single strands, and a single-stranded DNA was cycled to obtain a single-stranded cyclic DNA library with a single bridge primer.