Interkingdom plant-microbial ecological network analysis under different soil disturbance types in a tropical rainforest
Soil bacterial DNA extraction was obtained and purified. 16S rRNA gene was amplified. The polymerase chain reaction (PCR) mixture (total reaction volume, 20 L) comprised 10 ng of DNA template, 2 L dNTPs, 0.8 L of each primer, 4 L 5  FastPfu Buffer, 0.2 L BSA and 0.4 L FastPfu Polymerase (TransGen, China). The cycling conditions were 95 C for 3 min; 27 cycles of 95 C for 30 s, 55 C for 30 s, and 72 C for 45 s; and followed by 72 C for 20 min. The PCR products were purified and run using a Miseq (Illumina, USA).
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