GSM5672368: ARV Drug Treatment 1; Rattus norvegicus; RNA-Seq
Total RNA was isolated using mini spil column from Qiagen Libraries were prepared according to Illumina's instructions In brief, first and second strand synthesis was performed using standard protocol of TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA). cDNA fragments ~400 bp were obtained on gel electrophoresis and followed by PCR amplification. cDNA library was sequenced using Illumina's HiSeq 2000 with four RNA-seq libraries per lane.