TCRalpha repertoire of Atlantic salmon (Salmo salar) :healthy presmolt gill
Libraries were prepared using the Nextera XT DNA Sample Preparation Kit (Illumina Inc., San Diego, CA, USA) using the standard protocol. Each library was then quality checked with the Agilent 2100 Bioanalyzer. Prior to pooling and sequencing, libraries were quantified through PCR using the KAPA Library Quantification Kits, according to the manufacturers instructions. Libraries were pooled (4nM) and were diluted to 1.8 pM and subsequently paired- end sequenced with an Illumina Next Seq550 platform and with 300 cycle run chemistry.
Gi5_S2