GSM5706471: KO-VP-rep3; Mus musculus; RNA-Seq
Colons were flushed with PBS to clear feces and slit open longitudinally. Tumors were homogenized in 1 ml of TRIzol (Invitrogen). Total RNA was extracted from the tissue using TRIzol® Reagent (Plant RNA Purification Reagent for plant tissue) according the manufacturer's instructions (Life Technology) and genomic DNA was removed using DNase I (TaKara). Then RNA quality was determined by 2100 Bioanalyser (Agilent) and quantified using the ND-2000 (NanoDrop Technologies). Only high-quality RNA sample was used to construct sequencing library. RNA libraries were prepared for sequencing using standard Illumina protocols