GSM5736394: H446, H3K27ac; Homo sapiens; ChIP-Seq
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with the specified antibodies using standard protocol. Precipitated DNA samples were used to prepare sequencing libraries with Ultralow V2 DNA-Seq Library Preparation Kit (NuGEN, 0344NB-A01), and DNA samples were sequenced with the Nextseq 500 Illumina genome analyzer.