GSM5236522: human_CS17_brachial_rep1; Homo sapiens; RNA-Seq
Human embryonic material (4-7 weeks of gestation) was obtained from the MRC/Wellcome-Trust (grant #006237/1) funded Human Developmental Biology Resource (HDBR57, http://www.hdbr.org) with appropriate maternal written consent and approval from the London Fulham Research Ethics Committee (18/LO/0822) and the Newcastle and North Tyneside NHS Health Authority Joint Ethics Committee (08/H0906/21+5). Embryos were freshly collected on Hibernate-E medium (A12486, Gibco) supplemented with 2% B-27 (17504001 ThermoFisher Scientific) and 2.5 mL/L Glutamax (3505006, Gibco) and kept on ice until further dissection. Trunk samples of single embryos were dissected as in (Delile et al., 2019. PMID 30846445) RNA libraries were prepared for sequencing using Chromium Single Cell 3' v3 protocol for the human and v2 for the mouse; cDNA amplification involved 12 PCR cycles