GSM5235725: XY mESC rep 1; Mus musculus; RNA-Seq
RNA was extracted using the Direct-zol RNA miniprep kit cat#R2051 from Zymo Research RNA integrity (RIN) was measured for all samples using a Bioanalzyer Agilent 2100. All sequencing libraries analyzed were generated from RNA samples with a RIN score ≥ 9. The Illumina TruSeq Stranded Total RNA Gold protocol was used to deplete cytoplasmic and mitochrondrial rRNA from all samples, capturing coding RNA and multiplex forms for non-coding RNA. A total of 200ng of total RNA was used to construct RNA-seq libraries, and these were quantified and normalized using the Library Quantification Kit from Kapa Biosystems