Full genome sequence of Middelburg virus detected in human CSF sample
Extracted concentrated RNA was converted to cDNA and amplified using the modified SISPA (RACE) technique described previously to enhance coverage of terminal ends. Synthesised cDNA was sent to NICD Sequencing Core Facility (National Institute for Communicable Diseases, Sandringham, South Africa) for sequencing. Paired-end libraries were prepared using the Nextera DNA Flex library preparation kit, followed by sequencing (2 x 300 bp) on a MiSeq instrument (Illumina, San Diego, CA, USA).
ZRUH399-17 R1