GSM6069147: AG120 treated I1DN LSCs, scRNAseq; Mus musculus; RNA-Seq
GSM6069147
I1DN progenitor cells (cKit+/Cd11b-) from mice were FACS sorted into PBS/2%FBS. Cells were washed once with RNase free PBS/1%BSA and resuspended in PBS/1%BSA+0.2U/ml RNase inhibitor (Protector RNAse inhibitor, Sigma) at a final concentration of 1000 cells/uL. Cell suspensions were loaded onto separate channels of the 10XChromium Single Cell Chip and single cells were captured in droplet emulsions using the 10X Chromium Single-Cell Instrument. Reverse transcription, cDNA amplification and library preparation were performed using the Chromium Single Cell 5′ Library & Gel Bead Kit (10X Genomics). Libraries were pooled and sequenced on the Illumina NovaSeq 6000 system with 150 bp paired end reads to a depth of ~50,000 reads per cell.