GSM5238675: GFP-MatKD_rep3_SmallRNA [41550_rep3]; Drosophila melanogaster; ncRNA-Seq
Total RNA extracted using TRIzol, then small (20-30nt) RNAs extracted by size selection on 15% polyacrylamide gel TRIzol extraction from whole embryos, standard protocol following manufacturer's instructions RNA-seq (stranded) or Small RNA-seq; Illumina TruSeq Small RNA Library Prep Kit or Illumina TruSeq Stranded mRNA Library Prep kits For small RNA-seq raw data, only Read 1 analyzed and submitted because read is so short that Read 2 is the reverse complement of Read 1