whole genome sequencing of Plasmodium falciparum: human blood
DNA extracted from DBS was enriched for P. falciparum DNA before library prep using selective whole genome amplification (sWGA) reactions. The products of the sWGA reactions were pooled in equal volumes and acoustically sheared using a Covaris E220 instrument before library preparation using Kappa Hyper library preps. The indexed libraries were pooled and sequenced on a HiSeq 4000 using 2  150 chemistry at the University of North Carolina High Throughput Sequencing Facility.
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