RNAseq of Spea multiplicata: whole tadpole carnivore
The tadpole was euthanized, then stored in RNAlater at -80C. The whole tadpole was homogenized in TRIzol reagent and total RNA was extracted using the PureLink RNA Mini Kit (Invitrogen). The total RNA was then sent to Novogene (Sacramento, CA, USA) for library preparation and sequencing. Sequencing was performed on a NovaSeq 6000 (Illumina) in paired end mode with the read length 2x150bp.
BC273