RNAseq of Archegozetes longisetosus: protonymph females
RNA was extracted using a combination of the TRIzol RNA isolation protocol (Life Technologies) and RNeasy Mini Kit (Qiagen). The library was prepared with the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina together with NEBNext Multiplex Oligos for Illumina (New England Biolab) for library preparation, including reverse transcription of poly(A) RNA, amplification full-length cDNA, fragmentation, ligation and final library amplification. The library was sequenced with on an Illumina HiSeq2500 platform (single-end with read lengths of 50 bp) with ~18 million reads.
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