GSM5733730: ChIP H3K27ac KPC2; Mus musculus; ChIP-Seq
cells were harvested and crosslinker with 1% formaldehyde for 10 min at room temperature. After sonication, immunoprecipitation was performed with anti-H3K27ac (Abcam, Ab4729, 5 μg). The immunoprecipitated complex was washed, and DNA was extracted and purified by Universal DNA Purification Kit (#DP214). The ChIP-seq library was prepared using ChIP-Seq DNA sample preparation kit (NEBNext® Ultra™II DNA)