AsCas12a-Ultra targeting ARL16 in Jurkat cells using ARL16-98-AS guide RNA in the presence of ssDNA donor template
Targeted loci were amplified with a single primer pair; subsequently, universal amplification was used to install unique index sequences. Illumina sequencing was carried out on a MiSeq using V2 chemistry and 2x150 paired-end reads. Primer sequences and protocols are described in the manuscript.
20190815-CP3NP-S75