5-prime enriched transcriptome of Paulinella micropora KR01: Light sample
Paulinella micropora KR01 cells were grown at 23C in Fernbach flasks containing 0.75 L DY-V medium on a 12 h:12 h light/dark cycle at a light intensity of roughly 15-20 micromole photons m-2 s-1. Sample was collected an hour after the light was turned on. RNA was extracted using the Qiagen RNeasy Plant Mini Kit according to the Purification of Total RNA from Plant Cell instructions in the manual (Qiagen, Hilden, Germany) and the sample was enriched for 5-prime-complete transcripts using the SMARTer PCR cDNA Synthesis Kit (Clontech Laboratories, USA), following the manufacturers instructions.
Paulinella micropora KR01 5-prime enriched transcriptome - Light