GSM4781340: WT-wounded, replicate 2; Arabidopsis thaliana; RNA-Seq
Total RNA from all samples was purified with ReliaPrep TM RNA Tissue Miniprep System (Promega, Madison, WI). RNA quality was assessed with a Fragment Analyzer (Agilent, Santa Clara, CA). RNA-seq libraries were prepared from 500 ng of total RNA with the Illumina TruSeq Stranded mRNA reagents (Illumina) using a unique dual indexing strategy, and following the official protocol automated on a Sciclone liquid handling robot (PerkinElmer). Libraries were quantified by a fluorometric method (QubIT, Life Technologies) and their quality assessed on a Fragment Analyzer (Agilent Technologies). Cluster generation was performed with 2 nM of an equimolar pool from the resulting libraries using the Illumina HiSeq 3000/4000 SR Cluster Kit reagents and sequenced on the Illumina HiSeq 4000 using HiSeq 3000/4000 SBS Kit reagents for 150 cycles (single end).