Metatranscripome sequencing (RNA-seq) of cellulose-enriched community from Atta colombica refuse dump at transfer 14: enrichment line 1A
Total community RNA was extracted using previously published protocols (Book AJ, Lewin GR, McDonald BR, Takasuka TE, Doering DT, Adams AS, Blodgett JA, Clardy J, Raffa KF, Fox BG, Currie CR. 2014. Cellulolytic Streptomyces strains associated with herbivorous insects share a phylogenetically-linked capacity for the degradation of lignocellulose. Appl Environ Microbiol 80:46924701.). The Ribo-Zero Gold rRNA Removal kit (Epidemiology) was used to remove rRNA (Epicenter, Madison, WI). cDNA libraries were constructed, and Illumina HiSeq2500 Rapid Run 2x150 bp technology was used to sequence samples at the University of Wisconsin-Madison Biotechnology Center.
1A_T14_metatranscriptome