GSM5673237: Control 4; Homo sapiens; RNA-Seq
Left ventricular myocardial tissue was snap frozen in liquid nitrogen and RNA was isolated using the AllPrep RNA Kit (Qiagen). For library preparation, 1 ug of RNA was poly(A) selected, fragmented and reverse transcribed with the Elute, Prime, Fragment Mix (Illumina). A-tailing, adaptor ligation, and library enrichment were performed as described in the TruSeq Stranded mRNA Sample Prep Guide (Illumina).