GSM4905002: Leaf protoplast scRNA seq- Library 1; Arabidopsis thaliana; RNA-Seq
Two biological replicates were performed with the aim of capturing ~7,000 leaf protoplasts for each replicate. Freshly isolated protoplasts were adjusted to 700-900 cells/μL and loaded into the 10X Genomics Chromium single cell microfluidics device according to the Single Cell 3' Reagent Kit v2 protocol (10x Genomics, California). scRNA-seq library sequencing was performed on a NextSeq platform (Illumina Inc, California), using the sequencing parameters 26,8,0,98. Libraries were quantified using the NEBNext Library Quantification Kit for Illumina (New England Biolabs, Massachusetts). Libraries were made  according to the Single Cell 3' Reagent Kit v2 protocol (10x Genomics, California).