JMJD-1.2 OE in CEPsh glia, repeat 1
A total of 1000-2000 animals were harvested using M9, and animals were pelleted by centrifugation. M9 was subsequently aspirated and replaced with TRIzol solution. Worms were freeze-thawed 3 with liquid nitrogen, and a ~30-s vortexing was performed before each refreeze. After the final thaw, chloroform was added at a 1:5 ratio (chloroform:TRIzol), and aqueous separation of RNA was performed via centrifugation in a heavy gel phase-lock tube (VWR, 10847--802). The aqueous phase was collected, mixed with isopropanol at a 1:1 ratio, and then RNA purification was performed using  a QIAGEN RNeasy Kit as per the manufacturers directions. Library preparation was performed using Kapa Biosystems mRNA Hyper Prep Kit. Sequencing was performed at the Vincent J. Coates Genomic Sequencing Core at the University of California, Berkeley using an Illumina NovoSeq SP SR100.
ID5