GSM6212607: siRNAaRNF4_untreated_4; Homo sapiens; RNA-Seq
GSM6212607
RNA was extracted with Rneasy Mini Kit (Qiagene) 12 RNAseq libraries (NEBNext UltraII Directional RNA Library Prep Kit for Illumina, cat no. E7760) were produced according to manufacture protocol using 800 ng total RNA. mRNAs pull-up was performed using Magnetic Isolation Module (NEB, cat no. E7490). All 12 libraries were mixed into a single tube with equal molarity. The RNAseq data was generated on Illumina NextSeq500, 75 cycles, high-output mode (Illumina, cat no. FC-404-2005).