WGS of Vicia sativa
1.0 g of purified genomic DNA was used to construct an Illumina sequencing library using the NEBNext DNA Library Prep Kit following the manufacturer's protocol. Genomic DNA was randomly fragmented to an average size of 350 bp by shearing, DNA fragments were end polished, A-tailed, and ligated with the NEBNext adapter for Illumina sequencing. Then the library PCR products were size selected using AMPure XP beads and the resulting libraries were analyzed for size distribution using an Agilent 2100 Bioanalyzer. The library was quantified using real-time PCR before Illumina sequencing.
vetch_WGS_illuminia