Full-length 16S amplicon sequencing of Broiler litter stcokpile
Library preparation and full length 16S rRNA gene amplicon sequencing was performed at the Loop Genomics, USA (protocols available at www.loopgenomics.com). The barcoded 16S rRNA gene fragment sequences enable sequencing by short-reads on an Illumina sequencing platform, with subsequent reconstruction of the full-length 16S rRNA genes. Therefore, all hypervariable regions (V1V9) can be identified and analyzed because the entire 16S rRNA gene is sequenced.
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