GSM5733039: Genome_Wide_CRISPR_Screen_TF1_LZTR1-KO_Day0_Replicate2; Homo sapiens; OTHER
Cell pellets were lysed and genomic DNA was extracted (NucleoSpin Blood XL, Takara) and quantified by Qubit (ThermoScientific). A quantity of gDNA covering 1,000X representation of gRNAs was PCR amplified using TaKaRa Ex Taq® DNA Polymerase (Takara) to add Illumina adapters and multiplexing barcodes. Amplicons were quantified by Qubit and Bioanalyzer (Agilent), multiplexed, and sequenced on an Illumina HiSeq 2500 to obtain approximately 25e6 75b.p. single-end reads per sample.