Genomic sequencing of Brienomyrus brachyistius: skeletal muscle flowcell 1
Tissue samples for Pacific Biosciences and Hi-C chromatin mapping were isolated from a single an adult male Brienomyrus brachyistius obtained through the pet trade from an unknown location.  Sex was verified post-mortem by inspection of gonads (BBRACH_T26).  High molecular weight genomic DNA was isolated from skeletal muscle tissues using a Circulomics NanoBind Tissue Big DNA Kit (NB-900-701-01) following the Fish Skeletal Muscle Application Note v1.   Extracted gDNA was verified for concentration and quality using a Nanodrop and Qubit prior to submission for sequencing at Hudson Alpha Institute for Biotechnology.  Whole brain tissue was flash frozen in liquid nitrogen and shipped to Phase Genomics for Hi-C library preparation and sequencing.  Weestimated the size of B. brachyistius genome to be about 1 Gb, and thus utilized this estimate to generate coverage models for sequencing.   We targeted 20x coverage of processed CCS reads using two Pacific Biosciences SMRT cells.  Effective coverage was 25.3Gb.
BBRACH_T76_SM_1