GSM5939554: H1-EOS_primed; Homo sapiens; RNA-Seq
GSM5939554
For sample preparation, primed state cells were dissociated by TrypLE™ Select CTS™, and CD75+/SUSD2+ naive state cells were sorted in by using FACS. Total RNA was isolated by using the RNeasy Mini kit (Qiagen) and purified with the RNA Clean & Concentrator-5 kit (Zymo Research) according to the manufacturer's instructions. Polyadenylated RNA (polyA) was enriched by using NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England BioLabs). RNA libraries were prepared for sequencing using standard Illumina protocols