Genome-Seq of DSM 110487
Briefly, for preparation of 10kb libraries 1g genomic DNA was sheared using g-tubes from Covaris, Woburn, MA, USA according to the manufacturers instructions. DNA was end-repaired and ligated to barcoded adapters applying components from the SMRTbell Express Template Prep Kit 2.0 from Pacific Biosciences, Menlo Park, CA, USA. Reactions were carried out according to the manufacturers instructions.
110487-PacBio