GSM5593744: Hippocampus_EE1; Mus musculus; RNA-Seq
Mice were sacrificed via carbon dioxide asphyxiation and cervical spinal dislocation. Hippocampus was dissected, snap-frozen in liquid nitrogen, and stored at -80°C. RNA was isolated using AllPrep DNA/RNA Mini Kit (Qiagen) and stored at -80°C. RNA libraries were prepared for sequencing using the Illumina TruSeq Stranded total RNA Library Preparation protocol with rRNA depletion using RiboZero Gold.