GSM4904885: preHSC-hic-rep5; Mus musculus; Hi-C
Different cell populations sorted by FACS and fixed with 1% formaldehyde solution at room temperature for 10 min. MboI enzyme (NEB, R0147M) was used to digest chromatin. After biotinylation, ligation and proteinase K treatment, DNA was purified and sheared to 300-500 bp with Covaris M220, followed by Dynabeads Myone Streptavidin C1 bead pulling down. The Hi-C library were amplified 12-15 cycles with Extaq (Takara). Size selection was performed by adding 0.48X volume beads for the first time and 1X volume beads for the second time to get 200-1000 bp DNA fragment.