GSM5737352: ChIP_Cinobufagin_0nM_K27ac; Homo sapiens; ChIP-Seq
ChIP assay were performed using approximately 107 HL60 cells. Procedures were taken as reported previously[ A. Kaneda et al, PLoS Genet, 2011]. Antibodies against H3K4me3 (#ab8580, Abcam), H3K27ac (#39135, Active Motif) and Protein G-sepharose beads (GE Healthcare) were used. Chromatin-immunoprecipitated DNA was dissolved in EB buffer (Qiagen, Hilden, Germany). ChIP-seq Libraries were constructed using the KAPA Hyper Prep Kit (KAPA Biosystems) according to the manufacturer's instructions and sequenced at a concentration of 1.5 pM on Illumina Nextseq500 platform (Illumina, San Diego, CA, USA).