GSM5706218: KrasWT_7 (crc); Mus musculus; RNA-Seq
Animals were sacrified when they became moribund.Tumor lawns along colon were resected or scraped using a razor blade. Tumor tissues were flash frozen in liquid nitrogen and stored at -80ºC. Frozen samples were powderized in liquid nitrogen with a tissue homogenizer. RNA was extracted using the RNeasy Plus Universal kit from Qiagen, following the manufacturer's instructions. RNA concentration and quality were evaluated in Agilent Bioanalyzer/TapeStation. 1µg of RNA was used for RNA-Seq library preparation. Poly-A enrichment was performed to increase mRNA amount in the input RNA. RNA-Seq library was prepared using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® following the manufacturer's instructions.