GSM5670846: Viral genome sequencing dataset: PR8-infected IFNAR-/- mouse #4 rep#1; Influenza A virus (A/Puerto Rico/8/1934(H1N1)); OTHER
Whole lungs were homogenized in QIAzol. The total RNA was extracted using miRNeasy Mini Kit (Qiagen) with on-column DNA digestion using RNase-free DNase (Qiagen). Viral genomic RNA (vRNA) in the lung homogenate was amplified using multi-segment reverse-transcription PCR (M-RT-PCR) and then subject to Illumina library preparation with Nextera XT DNA library prep kit.