GSM5737301: C3; Mus musculus; ATAC-seq
The tumors were digested by collagenase and dispase, and CD45+ cells were be removed before sorting. Live tumor cells with EGFP+ were sorted for ATAC-seq library preparation. Illumina Tagment DNA Enzyme and Buffer Small Kit was used for library preparation with 1% Digitonin and Proteinase inhibitor cocktail.