GSM5740164: 10h00-50-C; Populus sp. cv. 'Okanese'; RNA-Seq
GSM5740164
Leaves for transcriptome analysis were harvested at 10:00 (late morning) and 16:00 (early evening), flash frozen in liquid nitrogen, and stored at -80°C until further processing. Leaf tissue was ground to a fine powder under liquid nitrogen and total RNA was extracted using RNeasy Plant MiniKits (Qiagen, Toronto, Canada). RNA was sent to Genome Québec (Montréal, PQ, Canada) where NEBNext stranded sequencing libraries were prepared according to manufacturer's instructions (New England Biolabs, Whitby, ON, Canada) and single end 100 base pair reads were sequenced on an Illumina HiSeq4000. A minimum of 23 million reads were generated for each library.