GSM5945027: Sp_blastula_AB1_ChIPseq; Strongylocentrotus purpuratus; ChIP-Seq
GSM5945027
Embryos at 24hpf (blastula stage) were treated with 1% formaldehyde for 12 min. Embryos were homogenized using Dounce homogenizer. Chromatin was fragmented to an average size of 100-200bp using a Covaris S1 ultrasonicator. Illumina ChIP-seq DNA Sample Prep Kit instructions (Catalog number IP-102-1001) were used to prepare the library. The quantity and quality (size distribution) of the libraries was assessed using an Bioanalyzer (Agilent). Libraries were multiplexed and sequenced on Illumina HiSeq 2500 to generate 50bp SE reads.