Winery walls before the receipt of grapes (2018)
The nuclear ribosomal internal transcribed spacer 2 (ITS2) region of the fungal DNA was amplified for this analysis using a mono index approach. The fusion prim- ers selected for this study were 5.8S-Fun (5-CAAGCAGAAGACGGCATACGAGAT-NNNNNNNNN NNN-AGTCAGTCAG-GG-AACTTTYRRCAAYGGATCWCT-3) and ITS4-Fun (5-AATGATACG GCGACCACCGAGATCTACAC-TATGGTAATT-AA-AGCCTCCGCTTATTGATATGCTTAART-3). The fusion primers contained the 24- to 29-bp Illumina sequencing adaptor, the 12-bp Golay barcode (5.8S-Fun exclusively), a 10-bp primer pad, a 2-bp linker, and the 21- to 27-bp core primer
50F