GSM5740389: 14D-Keto1; Mus musculus; RNA-Seq
GSM5740389
Cardiac endothelial cells were isolated using CD31 antibodies coupled to Dynabeads to separate cells through magnetic isolation. RNA was extracted using the RNAeasy mini kit (Qiagen). Libraries were prepared with an input of 10 ng RNA per sample using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara). Samples were sequenced on a HiSeq 2000 v4 system (Ilumina) with a depth of 50 base pairs in single-read mode. SmarTer Ultra Low Input RNA and NEBNext ChIP-Seq