GSM5593453: GPIDay25; Mus musculus; RNA-Seq
Freshly sorted synovial cells were combined with reagents from the 3' GEM Kit v3.1 (10X Genomics) and each sample was loaded on an individual lane of a 10X Genomics Cromium for targeted capture of 10,000 cells each. Following single cell partioning, cDNA was generated and further processed according to the manufacturer's recommended protocol. Following the amplification and size selection of cDNA according to the manufacturer's recommended protocol (10X Genomics 3' GEM Kit v3.1 and 3' GEM Library Kit v3.1), each sample was indexed using the i7 Multiplex Kit (10X Genomics) and then pooled at an equimolar ratio for sequencing.