GSM5706914: hom  α-Amanitin treatment 4h; Mus musculus; RNA-Seq
Cells in culture were treated with 15 μg/ml of the RNA Pol II and Pol III inhibitor α-Amanitin (Santa Cruz) or equal volume of PBS (vehicle) for 0 h and 4 h. Then the cells were collected after washing with PBS twice and processed for RNA extraction mRNA were used for library construction by using the NEBNext® Ultra™ II RNA Library Prep Kit (NEB) following manufacturer's recommendations.