GSM5014405: EC_smallRNA; Vitis vinifera; miRNA-Seq
Small RNA (<200 nt) was purified from 50 mg each of embryogenic cell cultures and young leaf tissue, using the Plant microRNA Purification Kit (Norgen BioTek). Library construction was performed by New Zealand Genomics limited, including isolation of 18-35 nt RNA fragments by polyacrylamide gel electrophoresis followed by standard preparation with the Illumina TruSeq Small RNA Library Preparation kit Small RNA libraries were prepared for sequencing using standard Illumina protocols