Illumina sequencing of Vibrio vulnificus Vv3
Pure cultures of the bacteria grown on TSA-1 were identified at species level by PCR using primers for vvha and API-20E system (BioMerieux, Madrid, Spain). Genome from selected tilapia CladeT V. vulnificus strain was extracted using GenEluteTM Bacterial Genomic DNA kit (Sigma) and NucleoBond Xtra Midi (Machery Nagel). DNA integrity was checked by electrophoresis and Nanodrop technology. DNA was quantified using Invitrogen Qubit 3.0 fluorometer (Thermo Fisher Scientific, Waltham, MA, USA) Next, DNA was sequenced using Illumina MiSeq.  Next, DNA was sequenced y FISABIOs Sequencing Service, with the Illumina NextSeq platform using Nextera XT Library Preparation Kit and manufacturers protocols (Illumina, San Diego, CA, USA), which generates 150 bp paired-ends DNA sequences.
Vv3_str