RNA-seq of Vicia sativa: root tip
RNA was purified from tissue using Spectrum Plant Total RNA Kit (Sigma Aldrich) with additional DNase I treatment to remove DNA contamination (On-Column DNase I Digestion, Sigma Aldrich), and ribosome removal treatment to enrich for non-ribosomal RNA fraction (RNARibo-Zero rRNA Removal Kit for Plant Leaf or Plant Seed/Root, Illumina). RNA libraries were prepared for each tissue using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturers protocols
Vs_root_tip