Direct RNA sequencing of triticale (Triticosecale)
Poly-A+ mRNA was purified from 100 g of total RNA by Dynabeads mRNA DIRECT Kit (ThermoFisher Scientific, USA) following the manufacturer's instructions. Final poly-A+ RNA concentration was measured using Quantus Fluorometer (Promega Corporation, USA) and checked by gel electrophoresis. For Nanopore sequencing Library was prepared from 1 g Poly-A+ using the nanopore Direct RNA Sequencing Kit SQK-RNA002 (Oxford Nanopore Technologies, United Kingdom). Sequencing was carried out using MinION and flow cell FLO-MIN106. Basecalling was peformed by Guppy (Version 4.0.11).
run1