GSM5238820: Ypel4-/- #2 Ortho-E; Mus musculus; RNA-Seq
Total RNA was extracted from cells using RNeasy Kit (QIAGEN) according to manufacturer's protocol. Genomic DNA was removed using DNase I on-column digestion (QIAGEN). RNA concentration and integrity were measured using Agilent RNA Pico Kit on a Bioanalyzer Instrument (Agilent Genomics). RNA sequencing libraries were prepared using TruSeq RNA Library Preparation Kit v2 (Illumina) according to manufacturer's standard protocol and the sequencing was performed using NextSeq 500/550 Mid Output v2 Kit, 150 cycle (Illumina).