GSM5733990: day7_30ugVac_4; Macaca mulatta; RNA-Seq
Single-cell RNAseq libraries were generated using the Next GEM Single Cell 5′ Library and Gel Bead Kit v1.1 (10x Genomics) and the Chromium Analyzer (10x Genomics) according to the manufacture's protocol (User guide RevF) Indexed sequencing libraries were generated using the Chromium Single Cell 5' Library Construction Kit (User gude RevF). In short, sequencing libraries were generated by 1) transcript fragmentation and end-repair, 2) post-fragmentation size-selection using SPRIselect beads, 3) Adaptor ligation and 4) sample index PCR and SPRIselect cleanup