GSM4785577: ATACseq_ZFSssI_line1; Arabidopsis thaliana; ATAC-seq
ATAC-seq libraries were prepared as previously described (Potok et al., 2019). Briefly, inflorescence tissues were first collected for nuclei extraction as previously described (Hetzel et al.,2016, Liu et al., 2018). Then transposition reaction was conducted in 25 µL of 2×DMF buffer (66 mM Tris-acetate (pH = 7.8), 132 K-Acetate, 20 mM Mg-Acetate, 32% DMF) mixed with 2.5 µL Tn5 and 22.5 µL nuclei suspension and incubated at 37 °C for 30 min. Transposed DNA fragments were then purified with ChIP DNA Clean & Concentrator Kit (Zymo, cat. no. D5205). Libraries were then amplified as described before (Potok et al., 2019, Buenrostro et al., 2013) .