GSM5235743: WT_3D_F3; Homo sapiens; RNA-Seq
Column purification using RNeasy Micro Kit (74004, Qiagen) was used to extract total RNA with DNASE-I treament to remove gDNA. RNA library preparation with polyA selection for sequencing with Illumina HiSeq, 2x150bp configuration, single index, per lane. ~350M raw paired-end reads per lane per sample was provided after sequencing