GSM4785638: hPO1805; Homo sapiens; RNA-Seq
Organoids were dissociated using TrypLE Select. Then, cells were further dispersed by gentle pipetting, filtered through a 30 μm cell strainer, counted by hemocytometer and resuspended in PBS + 0.01% BSA. Single cell RNA-seq that was performed using Drop-seq technology as described in Macosko et al. Libraries were prepared from 600 pg of of cDNA using the Nextera XT DNA sample prep kit according manufacturer's instruction using a custom Read 1 primer (GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC, IDT) and were sequenced on a NovaSeq 6000 machine (Illumina).