enCas12a-IDT NLS RNP targeting HPRT in HEK293 cells using crRNA8, Rep2
Targeted loci were amplified with a single primer pair; subsequently, universal amplification was used to install unique index sequences. Illumina sequencing was carried out on a MiSeq using V2 chemistry and 2x150 paired-end reads. Primer sequences and protocols are described in the manuscript.
20210120-JD92K-S392