GSM4969652: DIPG1114-RNAseq-rep1; Homo sapiens; RNA-Seq
Cells were harvested at 80% confluency and washed with PBS twice. Cells were homogenized with QIAshredder (79654, Qiagen). Homogenized whole cell lysate then were used for RNA isolation via the manufacturer's protocol (74104, Qiagen). Extracted RNA was stored in -80°C or proceed directly to library construction. RNA-Seq libraries were prepared using the Illumina TruSeq Stranded Total RNA Preparation Kit (RS-122-2201) with ribo-depletion. Input RNA quality was validated using the Agilent RNA 6000 Nano Kit (5067-1511). A total of one mg total RNA was used as starting material. 1 ug of RNA was used as starting material.