GSM5734066: DMSO treated zebrafish larval kiney hematopoietic cells rep3; Danio rerio; RNA-Seq
The cells in the 3.5 dpt kidney of DMSO or MTZ groups were labeled by red color via irradiating Tg(coro1a:DenNTR) transgenic larvae by a 405-nm laser in an LSM880 confocal microscope for 30 seconds. After photoconversion, approximately eighty zebrafish larvae were collected, mixed, dissected, and homogenized in 1 ml 0.5% trypsin at 4 °C. The homogenized cell suspension was centrifuged at 1000x g at 4 °C for 5 minutes. Then, the supernatant was removed and the cell pellet was re-suspended in PBS, followed by cell sorting using flow cytometry (Moflo XDP, Beckman). A cDNA library was generated from fifty red Dendra2+ cells by using the Smart-seq2 protocol.