16 sDNA of sulfide-oxidizing bacteria
The library was prepared by an Illumina TruSeq DNA Sample Preparation Kit adding index adapter for later sample tracing. The 16S rDNA amplicon of the library was then amplified and sequenced on an Illumina MiSeq instrument (Illumina, San Diego, USA). A v3 MiSeq chemistry kit was applied in a paired end mode. After removing low-quality sequences with QC score < 25 and ambiguous base N, the remaining high-quality sequences were clustered into an operational taxonomic unit (OTU) at 97% similarity level via UCLUST method.
H12_R1