GSM4968723: control 1143 fibroblasts; Homo sapiens; RNA-Seq
Total fibroblast and iPSC RNA was isolated from cells using a PerfectPure RNA Cultured Cell Kit following the manufacturer's protocol, including on-column DNase treatment. RNA integrity was confirmed on the 2200 TapeStation and quantified with Qubit RNA HS Assay Kit. Only  RIN ≥ 9.4 was used for RNA-seq. Before library preparation, rRNA was depleted from 1 ug of total RNA using the Ribo-Zero rRNA Removal. Libraries were constructed using the KAPA Stranded mRNA-Seq Kit, according to the manufacturer protocol.