Single-cell TCR sequencing of T cells from multiple subject-timepoints barcoded before pooling: Reaction 5
PBMCs from each donor were stained with a panel of DNA-barcoded, fluorescently labeled dextramers that matched the donors' HLA alleles. Cells were also stained by donor-timepoint with unique hashtag/barcoded antibodies for downstream sample demultiplexing. Epitope-specific T cells (CD3+CD8+dextramer+ cells) were isolated using FACS and then subjected to scRNAseeq, CITEseq, and scTCRseq using the Chromium platform (10X Genomics, 5' kit version 2). Antibody barcode identities are available in PRJNA744851_SRA_BarcodeMetadata.tsv
2247115_JCC392_SJTRC_InfVaxBT_2_Rxn5_TCR