GSM4969096: Human_skin_fib_rep3; Homo sapiens; RNA-Seq
The total RNA of primary human dermal fibroblasts, extracted using the RNeasy mini kit (Qiagen), was quantified using Qubit RNA high sensitivity assay kit (Thermo Fisher Scientific) and RNA integrity number (RIN) was assessed using the LabChip GX RNA Assay Reagent Kit (Perkin Elmer). Libraries prepared using the TruSeq Stranded mRNA Kit (Illumina) were pooled and sequenced on a NextSeq 500 sequencer (Illumina) using NextSeq 500 High Output v2 kit and paired-end 75–base pair sequencing chemistry.