Illumina sequencing of Vibrio vulnificus Vv5
Pure cultures of the bacteria grown on TSA-1 were identified at species level by PCR using primers for vvha and API-20E system (BioMerieux, Madrid, Spain). Genome from selected tilapia CladeT V. vulnificus strain was extracted using GenEluteTM Bacterial Genomic DNA kit (Sigma) and NucleoBond Xtra Midi (Machery Nagel). DNA integrity was checked by electrophoresis and Nanodrop technology. DNA was quantified using Invitrogen Qubit 3.0 fluorometer (Thermo Fisher Scientific, Waltham, MA, USA). Next, DNA was sequenced using Illumina MiSeq. Library construction and sequencing of Vv5 were performed by SCSIE (Servei Central de Suport a la Investigaci Experimental) of the University of Valencia, using Illumina TruSeq DNA PCR-Free Sample Prep Kit following manufacturer's instructions, obtaining 250 bp-length reads.
Vv5_str