Illumina NovaSeq 6000 paired end sequencing; The transcriptomics of SLC18A3 positive cells from arcuate nucleus of rats in different pubertal development stages.
The transcriptomics of SLC18A3 positive cells from arcuate nucleus of rats in different pubertal development stages.
PND45_SLC18A3_Positive_Small_Rep1_p
Cells harvested from FACS were centrifuged at 10,000 g for sheath fluid removal, and suspended by 10 Î¼l lysis buffer (Cat. No. 635013, ClonTech Laboratories, Mountain View, CA, USA) containing with 40 U Recombinant Rnase Inhibitor (Cat. No. 2313Q, ClonTech Laboratories). RNA was then processed for reverse transcription using SMARTer Ultra Low Input RNA Kit for Sequencing v3 (Cat. No. 634852, ClonTech Laboratories). The libraries were constructed using KAPA Hyper Prep Kit (Cat. No. KK8504, Roche, Basel, Switzerland)