GSM4970101: sdlg-asil1-1-br1; Arabidopsis thaliana; RNA-Seq
Total RNA was extracted using the RNeasy Plant Minikit (QIAGEN), per the manufacturer's directions. Illumina-complatible libraries were made starting with 5 ug of total RNA, using Kumar et al. (Kumar R, Ichihashi Y, Kimura S, Chitwood DH, Headland LR, Peng J, Maloof JN and Sinha NR (2012) A high-throughput method for Illumina RNA-Seq library preparation. Front. Plant Sci. 3:202. doi: 10.3389/fpls.2012.00202) Supplemetary Methods 1 library preparation protocol, at the exception of the RNA and mRNA isolation: the polyA isolation from total RNA described in Supplementary Methods 2 was used and the NEXTflex ChIP-Seq Barcodes (BiooScientific) were used for Illumina-compatible adapters. Libraries were quantified using Quant-iT PicoGreen dsDNA Reagent (Grand Island, NY) and a Nanodrop ND-3300 instrument.  Phi-X174 DNA was spiked in to the library by the sequencing facility before cluster formation and sequencing.