GSM5733159: E11.5_Female_ECKO---_3; Mus musculus; Bisulfite-Seq
Female E10.5 and E11.5 gonads were extracted and digested in 0.05% trypsin. GFP positive single mPGCs were sorted into a 96 well skirtless plate, each well containing 3 µL of 0.2% Triton X-100 and 2U/µL RNaseOUT (Thermo Fisher Scientific). Cells were sorted into lysis buffer from Zymo MethylDirect Kit and bisulfite converted, and then constructed libraries with Zymo Pico Methylation Kit.