GSM5706899: Hom Dnmt1 RIP2; Mus musculus; RIP-Seq
For Dnmt1 ripseq, 10 million  cells were collected and crosslinking was carried out by adding formaldehyde to a final concentration of 1%.  Rabbit anti-Dnmt1 antibody (Cosmo Bio, BAM-70-201) was used for immunoprecipitation.For RNA m5C immunoprecipitation, total mRNA was extracted by Dynabeads™ mRNA DIRECT™ Purification Kit (Thermo Fisher Scientific). Then 1 μg mRNA was incubated with anti-5-methylcytosine antibody (Diagenode, MAb-081-100). For the precipitated RNA, both ribosomal RNA (rRNA) depletion-based method and polyA enrichment- based method were used. purfied mRNA were used for library construction by using the NEBNext® Ultra™ II RNA Library Prep Kit (NEB) following manufacturer's recommendations.