GSM6069170: Mutant control 2; Escherichia coli str. K-12 substr. MG1655; RNA-Seq
GSM6069170
Bacterial cells were then harvested by centrifugation at 8000 × g for 10 min. Total RNA was extracted using the QIAGEN miRNeasy Mini Kit (QIAGEN, Germany) manufacturer's protocol with one extra bead-beating step to completely lyse the bacterial cells. RNA libraries were prepared for sequencing using standard Illumina protocols