WGBS of HSCR:HSCR_4
DNA extraction from EPCs was performed by MasterPureTM Complete DNA and RNA Purification Kit (Lucigen, Wisconsin,USA). Then, Bisulfite conversion of genomic DNA samples (100 ng) was carried out using EZ DNA Methylation-LightningTM Kit (Zymo Research, California, USA). The quality of denatured DNA was evaluated using a RNA Pico Chip on Agilent 2100 Bioanalyzer (Agilent Technologies, USA). Library preparation of the extracted and bisulfite modified DNA was performed using TruSeq DNA Methylation kit (Illumina, USA). Libraries were assessed for quality assurance using the Qubit High-Sensitivity DNA kit on Agilent 2100 Bioanalyzer (Agilent Technologies, USA).
H39