GSM5766836: 4-hydroxybenzoate Replicate 2; Comamonas testosteroni; RNA-Seq
GSM5766836
Samples were pelleted by centrifugation at 5000 g for 10 min and stored overnight at -80 °C before total RNA extraction. Enzymatic lysis of the bacteria cells was conducted using lysozyme prepared in molecular grade TE buffer and extractions were conducted using RNeasy Mini Kit (Qiagen). Samples were sent to NUSeq Core Facility (Northwestern University, Chicago, IL) for TruSeq Total RNA-seq Library Prep and Illumina HiSeq Sequencing, 50 bp, single-end reads.