WGA followed by whole exome sequencing of mus musculus: Trp53-/- KrasG12D-dependent primary tumors and matched KrasG12D-independent relapse tumors
At least 1 _g of gDNA was shared for input. Library preparation, sequencing, and initial quality control was performed by Novogene (Beijing, China). Briefly, gDNA was randomly fragmented by sonication (Covaris) to DNA fragments of 180-280 bp followed by library generation and exome capture using SureSelectXT Mouse All Exon (Agilent), according to manufacturers instructions. Purification was done using AMPure XP (Beckman Coulter), according to manufacturers instructions. Quantification was done with the High Sensitivity DNA assay (Agilent) on the Bioanalyzer 2100 (Agilent), according to manufacturers instructions.
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