GSM6212730: pilB::Tn5 Mutant (Btn5) in Conditioned Media on Day 1, Replicate #3; Synechococcus elongatus PCC 7942 = FACHB-805; RNA-Seq
GSM6212730
Total RNA was extracted using a modified phenol-chloroform, LiCl, and ethanol precipitation protocol from Tu, et al. (2004). DNA was removed via DNase treatment, and the RNA was repurified using a phenol-chloroform and ethanol precipitation procedure. Total RNA was depleted of ribosomal RNA using the Bacteria Ribo-Zero rRNA Removal Kit (Epicentre) and purified using the RNeasy miniprep kit (Qiagen) according to the manufacturer's instructions. Sequencing libraries were prepared by The Scripps Research Institute Sequencing Core using a TruSeq Stranded Total RNA kit following the manufacturer's instructions.