GSM5014386: ATAC_WT_S1; Mus musculus; ATAC-seq
Keratinocytes RNA was extracted with the RNeasy Minikit (Qiagen) Nuclei were subject to transposition reaction in 1x TD Buffer containing 2.5 μl transposase Nextera enzyme and DNA was purified. Purified DNA was amplified by PCR with 10-12 cycles. Amplified libraries were purified and quality controlled.