GSM5129436: ibat_ctrl13; Mus musculus; RNA-Seq
Primary adipocytes were washed with ice-cold PBS, placed on ice and scraped into Trisure (Bioline). Total RNA was prepared from Trisure (Bioline) and column-purified (SV Total RNA Isolation System, Promega). Libraries were constructed from total RNA (TruSeq RNA Sample Prep kit v2 (Illumina)).