GSM5706918: Torin1-treated 1 [T1]; Homo sapiens; RNA-Seq
RNA samples were extracted using Qiagen RNeasy Plus kit (QIAGEN) Library construction and RNA sequencing were performed by Novogene as a service. Briefly, ribosomal RNA was removed from total RNA by rRNA removal kit, and rRNA free residue was cleaned up by ethanol precipitation. After fragmentation, the first strand cDNA was synthesized using random hexamer primers. Then the second strand cDNA was synthesized and dUTPs were replaced with dTTPs in the reaction buffer. The directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification.