GSM5766793: HepG2 input rep2; Homo sapiens; RIP-Seq
GSM5766793
Total RNAs were isolated from the whole cells with QIAzol and poly(A) RNAs were enriched from total RNA with oligo(dT) polystyrene beads.The anti-m7G antibody (Cat#: RN017M, MBL international) was applied for m7G immunoprecipitation. Both input and m7G IP samples were utilized for next-generation sequencing (NGS). The library preparation was constructed using the TruSeq Stranded mRNA Sample Prep Kit (Illumina) and was quantified by BioAnalyzer High Sensitivity DNA chip, and then was deeply sequenced on the Illumina HiSeq 2500.