Bait capture of Theileria parva: tissue culture
Fragmentation of the DNA was done using Covaris to get a size distribution of 125 bp. DNA end-repair, addition of a 3-A and ligation using SureSelect adapters was done followed by adapter-mediated PCR prior to in-solution hybridisation. Streptavidin Dynabeads were used to isolate the biotinylated baits with hybridised DNA fragments and host DNA was washed away. Captured DNA was subsequently amplified and indexed prior to sequencing.
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