GSM5237601: GUIDE-Seq SG3; Homo sapiens; OTHER
Genomic DNA was isolated (QuickExtract) followed by fragmentation and adapter ligation using the LOTUS™ DNA Library Prep Kit (IDT). Libraries were generated by two rounds of amplification, followed by Illumina-based NGS. Illumina sequencing was carried out on a MiSeq using V2 chemistry and 2x150 paired-end reads