GSM5739845: SEM3, 10, Cas9; Homo sapiens; RNA-Seq
GSM5739845
Total RNA was isolated using the RNeasy Kit (QIAGEN) including a DNAse treatment step RNA-seq libraries were prepared starting from 30 ng of total RNA using the Universal Plus mRNA-Seq kit (Nugen) as recommended by the manufacturer. Briefly, mRNA was captured with polyA+ magnetic beads from total RNA. mRNA was chemically fragmented. Single strand and second strand cDNA were produced and then ligated to Illumina compatible adaptors with UDI. To produce oriented RNA-seq libraries, a final step of strand selection was performed. The NuQuant system (Nugen) was used to quantify the RNA-seq libraries.