GSM5706725: WT_Angel+/+_rep1; Drosophila melanogaster; OTHER
The monosome fractions were pooled and RNA was extracted using Direct-zol RNA micro prep kit (Zymo Research) following manufacturer's instructions and incorporating an on-column RNase-free DNase digestion to remove DNA. RNA was dephosphorylated and phosphorylated with T4 PNK. RNA sequencing libraries for ribosome profiling were prepared using the Illumina Small RNA Sample Prep Kit, with a size selection step for 15-90 nt RNAs.