GSM5672499: WT; Mus musculus; RNA-Seq
Single cells were suspended in 0.04% non-acetylated BSA (UltraPure BSA, ThermoFisher Scientific) and filtered by Dead Cell Removal kit (#130-090-101, Miltenyi Biotec B.V. &Co.KG) so that debris and dead cell is excluded. Single-cell suspensions were captured using the 10X Genomics Chromium Single Cell 3' Solution (v3) according to the manufacturer's protocol. RNA libraries were prepared with Chromium Single cell 3' Reagent v3 Kits according to the manufacturer's protocol.