GSM5706947: DD1; Mus musculus; OTHER
In vitro transcribed pTRI-Xef RNA was used as negative control in the amplicon-bsRNA-seq experiments. RNAs were transcribed using the MEGAscript® T7 Kit (Life Technologies) according to the manufacturer's protocol. Total mRNA of mouse fibroblasts was extracted by the Dynabeads™ mRNA DIRECT™ Purification Kit (Thermo Fisher Scientific). Then 1 μg of the mRNA combined with 1 ng pTRI-Xef RNA were used for bisulfite treatment by EZ Methylation Kit (Zymo) following the protocols provided by the manufacturer. After bisulfite conversion, the purified RNA was used for library construction by NEBNext® Ultra™ II Directional RNA Library Prep Kit (NEB).