GSM5766332: 100_S25_L004_R1_001; Homo sapiens; RNA-Seq
GSM5766332
Total RNA was extracted from skin biopsy samples using Qiagen miRNeasy Mini kit. The libraries were generated using TruSeq Stranded mRNA Library Prep kit following manufactory protocol. Briefly, mRNA was first extracted from 240ng of total RNA using oligo-dT magnetic beads and fragmented at high temperature. A cDNA library was then prepared from the fragemented mRNA by revervse transcription, second strand synthesis and ligation of specific adapters.