GSM5129388: Col_21°C_H2A.Z_rep1; Arabidopsis thaliana; ChIP-Seq
Materials were ground with liquid nitrogen into fine powder and fixed with 1% formaldehyde. After nuclei extraction, mononucleosomes were generated with micrococcal nuclease (MNase) (Sigma, N5386) digestion. Immunoprecipitation was performed with an anti-H2A.Z antibody. After antibody incubation, Protein A Dynabeads (Life Technologies,10002D) were added to collect the immunocomplexes, followed by elution, reverse cross-linking and DNA purification. Library was prepared with VAHTS universal DNA library prep kit for Illumina (Vazyme, ND607) according to the manufacturer's instruction.