GSM5706880: female E13.5 Zfp57-/- (M-Z-) mRNA-Seq rep1; Mus musculus; RNA-Seq
GSM5706880
E13.5 Live E13.5 embryo were flash frozen on liquid nitrogen, and RNA was harvested using Trizol reagent. Qualified total RNA was further purified by RNeasy micro kit (Cat#74004, QIAGEN, GmBH, Germany) and RNase-Free DNase Set (Cat#79254, QIAGEN, GmBH) Germany).embryo were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols