GSM5945529: HDM-3; Mus musculus; RNA-Seq
GSM5945529
Lungs were removed, placed in RNAlater solution, cut into small pieces and flash frozen on Liquid nitrogen. Lung tissue was homogenized in Trizol, and total RNA was isolated using a Direct-zol RNA extraction kit. RNA samples were sequenced at GeneWiz Next Generation Sequencing facility using Illumina HiSeq platform. Libraries were prepared according to Illumina's instructions in 4 steps. These include 1) mRNA enrichment, mRNA fragmentation, and random priming, 2) first and second strand cDNA synthesis, 3) end repair, 5' phosphorylation, and dA tailing, and 4) adapter ligation, PCR enrichment and sequencing on the Illumina HiSeq following the manufacturer's protocols.