RNA-Seq of Vitis vinifera: red flesh at 40 days after bloom
Total RNAs of berry flesh were extracted using TRIzol Reagent (Invitrogen, Carlsbad, CA, USA) and mRNA was purified using poly-T oligo-attached magnetic beads. Sequencing libraries were constructed using a NEBNext UltraTM RNA Library Prep Kit for Illumina (#7530L, NEB, USA) according to the manufacturers instructions. After determinations of the RNA concentration and insert size and the clustering of the index-coded samples, the libraries were sequenced on an Illumina HiSeq Xten platform (Illumina, Inc., San Diego, CA, USA), and 150-bp paired-end reads were generated.
Red40_replicate3