GSM5739762: S_21_Rep2_MC38_Day_6_Untx; Mus musculus; RNA-Seq
Tumors were harvested at the indicated time points post-implantation.  Single-cell suspensions were created using a gentleMACS dissociator and associated digestion kit (Miltenyl).  Post-digestion ACK Lysis Buffer (Life Technologies) was used to eliminate red blood cells.  For cell sorting, after surface staining cells were resuspended in PBS supplemented with 2% FBS (Gibco) and sorted on a SH300S (Sony). RNA libraries were prepared for sequencing using standard Chromium platfrom from 10x Genomics 5' v2 kit.