GSM5237159: ChIP-MCF7_BRD4; Homo sapiens; ChIP-Seq
For ChIP-seq assay,the chromatin DNA precipitated by antibodies against BRD4, LSD1, MTA3, or H3K4me1. Then the DNA was purified with the QIAquick PCR Purification Kit. For RNA-seq assay, total RNAs were extracted from MCF-7 and MDA-MB-231 cells that were untreated or treated with JQ1 for 4 weeks using Trizol reagent. RNA and DNA libraries were prepared for sequencing using standard Illumina protocols