GSM5739716: healthy subglottis rep 1; Homo sapiens; RNA-Seq
GSM5739716
Samples were placed in an enzymatic cocktail [collagenase I/dispase II (1 μg/ml) tissue for 60 minutes followed by mechanical disruption. Tissue lysates were serially filtered 100- and 40-μm sterile filters (Fischer) and used as input for generation of scRNA-seq libraries. scRNA-seq libraries were generated using the 10x Chromium platform 3′ v2 or 5′ library preparation kits (10x Genomics) following the manufacturer's recommendations and targeting 5000 to 10,000 cells per sample. scRNA-seq with 10X Genomics Chromium platform