Fungal amplicon data for Oo Soil sample 3 replicate B
Whole DNA was extracted from soil sample using Qiagen PowerLyzer Power Soil extraction kits. ITS2 was amplified via PCR and a secondary limited cycle PCR was used to affix Illumina barcodes to each library. The concentration of each library was determined and normalised via dilution. Libraries were sequenced on an Illumina MiSeq machine at the University of Wisconsin-Madison.
Oo3_ITS2_B