GSM5173299: NN24h_2_input; Mus musculus; RNA-Seq
RNA was extracted from whole cochlear samples (input) and following immunoprecipitation using an HA antibody (IP) to enrich for outer hair cell transcripts or supporting cell transcripts following the protocol outlined in Sanz et al., 2009. RNA-seq libraries were prepared in biological triplicates using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturer's instructions.