GSM5945334: T16, H505, 3; Vibrio cholerae O1 biovar El Tor str. N16961; OTHER
GSM5945334
Part of the transposon mutant libraries were used for gDNA extraction using Qiagen DNeasy® Blood & Tissue Kit as per manufacturer's instructions. Sequencing libraries were prepared using Agilent's sureselect XT2 Kit with custom RNA baits designed to hybridize the edges of the Mariner transposon. The 100 ng protocol was followed as per manufacturer's instructions. A total of 12 cycles were used for library amplification. Agilent's 2100 bioanalzyer was used to verify the size of the pooled libraries and their concentration.