GSM4970298: H28126 - 9 weekRNA-Seq; Homo sapiens; RNA-Seq
Cerebral organoids were dissociated in Papain containing Deoxyribonuclease I (DNase) for 30 minutes at 37C and samples were triturated to form a single cell suspension. For scRNAseq cells were counted and loaded onto 10x chip. Single cell RNA-seq libraries were generated using the 10x Genomics Chromium 3' Gene Expression Kit. Briefly, single cells were loaded onto chromium chips with a capture target of 10,000 cells per sample. Libraries were prepped following the provided protocol and sequenced on an Illumina NovaSeq with a targeted sequencing depth of 50,000 reads per cell. BCL files from sequencing were then used as inputs to the 10X Genomics Cell Ranger pipeline.