GSM4905035: WT E6.5 rep1; Mus musculus; RNA-Seq
Embryos were collected, stocked in MeOH with 2 embryos/sample. Total RNAs were extracted using Trizol reagent (Thermo Fisher Scientific) and evaluated using Agilent Bioanalyzer with RNA 6000 Pico kit (Agilent Technologies). RNA libraries were prepared for sequencing using standard Illumina protocols. Total low-input RNA was used for rRNA-depletion and library synthesis by Takara SMARTer kit (Takara). RNA-seq library was prepared with Agilent Bioanalyzer, DNA High-sensitivity kit (Agilent Technologies) and performed with Illumina NextSeq500 (Illumina) by Tsukuba i-Laboratory LLP.