small RNASeq of Maize: germinating Seed
Small RNAs (15- to 40-nt) were isolated following the previous method (He et al., 2019). The small RNA libraries were constructed using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB, E7300S) kit and sequenced on the Illumina HiSeq 2500 platform at Berry Genomics (Beijing, China) to generate 50-bp single-end reads.
g-seed-sRNAseq-rep2