GSM5129444: fused SUM-159 replicate 2; Homo sapiens; RNA-Seq
Media was removed and cells were lysed with Trizol (Thermo) according to manufacturer's instructions.  A second CHCl3 extraction was performed to increase purity.  Total RNA was quantified by Nanodrop. RNA-seq libraries were made from 5 ng total RNA spiked with 1e-5 final dilution of ERCC Mix 1 (Thermo) using Ovation RNA-seq v2 kit (NuGEN) to make cDNA and Ovation Rapid DR Multiplex System (NuGEN) to make libraries according to manufacturer's instructions.