GSM5706274: WT.MEF.GS.12hr; Mus musculus; RNA-Seq
At the end of starvation, MEFs were collected using trypsin EDTA and resuspended in freezing media (10% DMSO + 90% dialyzed FBS). The control sample (0 hour starvation) was collected and resuspended in freezing media composed of 90% FBS instead of dialyzed FBS. Vials containing each sample were stored in a deep freezer before proceeding to sequencing. The viability of samples was checked using Countess and 5,000 cells from each sample were used for single cell isolation, library preparation and sequencing performed by Theragenetex. MEFs in starvation condition were collected using trypsin EDTA and resuspended in freezing media (10% DMSO + 90% dialyzed FBS). The control sample (0 hour starvation) was collected and resuspended in freezing media composed of 90% FBS instead of dialyzed FBS. All scRNAseq libraries were prepared using Single Cell 3' v2 chemistry 10X chromium Kit and sequenced using Illumina HiSeq 4000.