Genome sequence analysis of Tribolium castaneum-adapted E. coli Nissle 1917 (8x72h midgut persistence)
Total genomic DNA was isolated using the MagAttract HMW DNA kit (Qiagen, Hilden, Germany). To prepare 500 bp paired-end libraries of all isolates we used the Nextera XT DNA Library Preparation kit (Illumina, San Diego, CA, USA).
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