GSM5237093: mLN_GF_FSC_1 (RNAseq); Mus musculus; RNA-Seq
Total RNA was extracted from FACS-sorted CD45‑CD24‑CD31‑gp38+ using the RNeasy Plus Micro Kit (Qiagen, Cat. #74034). cDNA was synthesized and amplified using template switching technology of the SMART‑Seq v4 Ultra Low Input RNA Kit (Clontech Laboratories, Cat. #R400752), followed by purification using the Agencourt AMPure XP Kit (Beckman Coulter, Cat. #A63880). Library preparation was performed with Nextera XT DNA Library Prep Kit (Illumina). The Agilent Technologies 2100 Bioanalyzer was used to control quality and integrity of nucleic acids after each step. Deep sequencing was carried out on Illumina HiSeq2500 using 50 bp single reads. Sequenced libraries were assessed for read quality using the FastQC tool.