GSM5943556: Hashtagged antibody barcode from Patient 3 & 4 [HTO]; synthetic construct; RNA-Seq
GSM5943556
Single cell transcriptomic libraries were generated using the 5'v2 Gene expression and immune profiling kit (10x Genomics). Viable PBMCs were sorted into 2% FBS/PBS and cell counts were performed using a haemocytometer. Up to 40,000 cells were loaded into each lane of Chromium Next GEM Chip K Single Cell Kit (10x Genomics) to achieve a recovery cell number of approximately 20,000 cells. cDNA and TCR libraries were generated according to manufacturer's instructions (10x Genomics). Generated libraries were sequenced on the NovaSeq S4 flow cell (Illumina) at Read 1 = 28, i7 index = 10, i5 index = 10 and Read 2: 90 cycles according to manufacturer's instructions.