Unigenes of transcriptome data obtained from Blaps rhynchopetera adult muscle and defense gland tissue
Colonies of the species B. rhynchopetera were collected from China (Yunnan, Binchuan). Abdominal defensive glands were dissected separately from adult B. rhynchopetera (reared at 28C, 30 days after eclosion). Thirty individuals were sacrificed, and their defensive glands were pooled in the same sample. The samples were immediately frozen in liquid nitrogen and stored at 80C until further process. In order to investigate differentially expressed genes, chest muscle tissues, where no glands and digestive tracts are located, were taken as control tissues. Total RNA was extracted from the muscle tissues and defensive gland tissues of the adult B. rhynchopetera, and the concentration. The mRNA from the total RNA were isolate and enrich using magnetic Oligo (dT) beads (Cat# 61002, ThermoFisher Scientific). The mRNA was randomly broken by adding fragmentation buffer, and the small fragments about 300 bp were isolated by magnetic-bead screening. By reverse transcription, random hexamers are added to synthesize the frist strand of cDNA using mRNA as a template, and then the second-strand was synthesized was conducted. End Repair Mix was added to compliment the cDNA into a flat end, followed by the addition of an A base at the 3' end for joining the Y-junction. The cBot System was used for bridge PCR amplification to generate clusters. The transcriptome was sequenced at 2150 bp using Illumina Novaseq 6000 to construct the Illumina PE libraries, and the sequencing data were generated under quality control.
BrMD