GSM4905105: PAC2_WT_rep1; Danio rerio; ATAC-seq
Fresh cells were lysed in lysis buffer (10 mM Tris-HCl at pH 7.4, 10 mM NaCl, 3 mM MgCl2 and 0.1% IGEPAL CA-630 [Sigma-Aldrich]) for 10 min on ice to prepare the nuclei. Immediately after lysis, nuclei were spun at 500 x g for 5 min to remove the supernatant. Nuclei were then incubated with the Tn5 transposome (Vazyme Biotech, China). Following the tagmentation at 37 °C for 30 min, the products were puriﬁed with QIAquick PCR Purification Kit (Qiagen, Germany), PCR was performed as described in a previous study [1]. After the amplification, libraries were purified with Agencourt Ampure XP beads (Beckman, USA) to remove contaminating primer dimmers and quantitated using Qubit 2.0 Fluorimeter (Invitrogen, USA). All libraries were sequenced on the Illumina HiSeq X Ten with 150 bp paired-end reads (Novogene Biotech, China)