GSM5058630: 5439-CJ-4; Mus musculus; RNA-Seq
Trizol reagent was added to cells plated on a p100 dish, mechanically agitated, then collected. RNA was extracted with a Zymo Direct-Zol Miniprep kit using the standard protocol. Libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kits according to standard protocols.