RNA seq of T. brucei Lister 427 with Fip1 kncokdown
Poly(A) RNA was isolated from total RNA of PF trypanosomes with a Dynabeads mRNA isolation kit. The integrity of RNA was evaluated in Agilent 2100 Bioanalyzer with a RNA 6008 nano kit. For short reads RNA sequencing, RNA was fragmentated with a magnesium RNA fragmentation kit (NEB) and size-selected, reverse  transcribed with random six primer.
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