GSM5706354: DDB1_KD_2; Sus scrofa; RNA-Seq
The RNA was extracted using a RNeasy Mini Kit (74034, Qiagen, Germany). A cDNA library was constructed using a TruSeq Nano DNA LT Library Preparation Kit (Illumina, FC-121-4001, USA). The cDNA was fragmented by dsDNA Fragmentase (NEB, M0348S, USA) by incubating at 37 °C for 30 min. The library construction began with the fragmented cDNA. Paired-end sequencing was performed on an Illumina 6000 sequence platform The libraries were prepared according to the transcriptome differential expression analysis of the NC group and the DDB1_KD group