GSM5673223: E11.5 Sox9+ Progenitor Cells - Sample 1 [ATAC-seq]; Mus musculus; ATAC-seq
A single cell suspension was generated from embryonic lungs isolated from Tg(Sox9-EGFP)EB209Gsat reporter mice at either E11.5 or E16.5. SOX9+ lung epithelial cells were isolated by sorting for GFP+/CD326+/CD31-/CD45-/7-AAD- using a BD/FACSAria II cell sorter. Nuclei were isolated, the transposase reaction was performed with Nextera Tn5 transposase (Illumina), and PCR amplification was performed according to previously published protocols (Buenrostro et al. 2015a). Nextera Tn5 transposase (Illumina) per previously published protocols (Buenrostro et al. 2015a).