GSM5777086: H3R17me2a_ChIP-seq; Homo sapiens; ChIP-Seq
GSM5777086
ChIP protocol was adopted from Myer's Lab at HudsonAlpha (protocol version v011014) which contributed to the ENCODE project. Except that: i), cells were lysed in ChromaTrap lysis buffer that designed for sonication (Porvair Science, 100001), and chromatin fragmentation was carried out on a Covaris E220 instrument with optimised settings for ChIP purpose; 2), ChIP-grade protein A/G beads (Pierce 26162) were used to perform immuno-precipitation experiments; 3), the following buffers were used for binding and washing of chromatin: H3R17me2a and CARM1 (100 mM Tris pH 7.5; 500 mM LiCl; 1% NP-40; 0.1% sodium deoxycholate); TDRD3 (100 mM Tris pH 7.5; 500 mM LiCl; 0.5% NP-40; 0.5% sodium deoxycholate); 4), the following antibodies were used: TDRD3 (Proteintech 13359-1-AP), H3R27me2a (Active Motif 39709), CARM1 (Active Motif 39251). Libraries were constructed by Beijing Genomics Institute Hong Kong, using standard Illumina TruSeq protocol.