GSM5238452: BjF_2_16PAH_Rep2; Danio rerio; RNA-Seq
Following exposure, 48-hpf whole embryos were homogenized using RNAzol (Molecular Research Center, Inc).  RNA was purified using the Direct-zol MiniPrep kit (Zymo Research) 100 bp sequencing was conducted using an Illumina HiSeq 3000 sequencer mRNAs were enriched by polyA selection. Libraries were prepared with the PrepX™ mRNA and Illumina sequencing workflow (Wafergen Biosystems)