GSM4790354: WT_Brain_RNAseq_rep3; Danio rerio; RNA-Seq
Brain and whole kidney were snap freezed. Total RNA was extracted using TRI reagent (T9424, Sigma-Aldrich) and treated with DNase1 to degrade genomic DNA.  Samples were purified with Quick-RNA Miniprep kit (R1054, Zymo Research). Brain and WKM nuclei were isolated through douncer homogenization or vigorous pipette respectively. 100,000 cells/nuclei were used for library preparation. RNA libraries were prepared for sequencing using standard Illumina protocols; ATAC library preparation was performed as described in (Buenrostro et al., 2013).