GSM4785468: d0_KO_54; Mus musculus; RNA-Seq
Total RNA was extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer's protocol. RNA concentration was measured using a NanoDrop ND-1000 spectrophotometer (PeqLab Biotechnologie). Total RNA was extracted as described from isolated IECs from Hk2∆IEC and littermate control mice under untreated conditions and after administering DSS for 3 or 7 days. RNA libraries were prepared using TruSeq stranded mRNA Kit (Illumina) according to manufacturer's instructions. All samples were sequenced using an Illumina NovaSeq 6000 sequencer (Illumina, San Diego,CA) with an average of 23 million paired-end reads (2x 50 bp) at IKMB NGS core facilities.