GSM5670782: RNA-seq dataset: DI222-treated IFNAR-/- mouse #3; Mus musculus; Influenza A virus (A/Puerto Rico/8/1934(H1N1)); RNA-Seq
Whole lungs were homogenized in QIAzol. The total RNA was extracted using miRNeasy Mini Kit (Qiagen) with on-column DNA digestion using RNase-free DNase (Qiagen), followed by poly(A) mRNA enrichment. The enriched poly(A) mRNAs were subject to RNA-seq library preparatin with NEBNext Ultra II RNA library prep kit for Illumina following the manufacturer's protocol.