RNA-seq of Chelicerate
We isolated total RNA using TRIzol reagent (Invitrogen) and purified mRNA with Dynabeads (Invitrogen). We assessed the quantity and quality of mRNA with the 2100 Bioanalyzer (Agilent technologies). For cDNA library construction, we used the PrepX RNA-Seq Library kit and the Apollo 324 System (Wafergen), assessing the success of library construction following 815X Polymerase Chain Reaction (PCR) amplification by measuring DNA concentration with qPCR (Kapa Library Quant Kit) and library fragment size distribution with the 4200 TapeStation. We pooled up libraries together at equimolar concentrations, confirmed pool concentration with TapeStation and qPCR and sequenced final pools on the Illumina HiSeq 2500 platform with paired ends of 150 bp at the Bauer Core Facility at Harvard University.
Gonyleptidae MCZ-IZ-139272