WGS Nanopore for Serratia marcescens: Felis catus urine
Genomic DNA purified with Promega Wizard HMW Genomic DNA Extraction Kit. Libraries prepared with rapid barcoding kit SQK-RBK004 (Oxford Nanopore) and loaded on a MinION MK-Ib device fitted with flowcell R9.4. Live rapid basecalling performed with MinKnow 20.13.3 using Guppy version 4.2.2 (Oxford Nanopore), followed by filtering of reads with quality > 7, demultiplexing and barcode removal with guppy_barcoder (Oxford Nanopore). Reads > 1 kb processed with Filtlong (https://github.com/rrwick/Filtlong) using corresponding Illumina datasets as refernce sequences.
CM2012_028_n