GSM5944990: D.shibae ∆191kb Replicate C; Dinoroseobacter shibae; RNA-Seq
GSM5944990
Bacterial cells were centrifuged and the pellets without remaining medium were frozen in liquid nitrogen, pestled, and stored in aliquots of about 150 mg at -80°C. The pestled bacterial cell powder was covered with 1 ml TRIzol reagent (ThermoFisher Scientific, Waltham, MA, USA) and incubated for 5 min at room temperature. The total RNA isolation starting with the centrifugation at 12,000 g for 10 min at 4°C were performed with the RNeasy mini kit (Qiagen, Hilden, Germany). Depletion of ribosomal RNA was performed with the mRNA Ribo-Zero Magnetic Kit (Epicentre, Madison, WI, USA). The library was prepared from ribosomally depleted total RNA using Scriptseq v2 RNA-Seq Library Preparation Kit (Epicentre, Madison, WI, USA).