GSM5237215: ChIP-seq DLD1 input; Homo sapiens; ChIP-Seq
For ChIP-seq samples, cells were fixed in 1% formaldehyde for 10 min at room temperature, quenched with 180mM glycine, and lysed in three steps according a protocol modified from Schmidt. et. al (PMID 19275939). Chromatin was sonicated to an average size of 250-300 bp with a Q800R2 sonicator (QSonica) at 4C. ChIP was performed with 100μg chromatin, 10μg antibody, and protein G Dynabeads (Thermo, #100003D) overnight. IPs were washed, reverse-crosslinked, treated with proteinase K/RNase A, and input and IP DNA was purified MinElute PCR Purification columns (Qiagen #28004). For ChIP-seq samples, libraries were prepared from input or IP DNA using the Swift Biosciences ACCEL-NGS 2S Plus DNA library kit following the manufacturer's protocol. Single-end 75-bp sequencing was performed using a high-output flowcell on the Illumina NextSeq 500 at a depth of 40-55M reads per sample