GSM5627747: hMSC_8013_Day7; Homo sapiens; RNA-Seq
Total RNA was isolated from Trizol with DNaseI treatment following manufacturers protocol (Invitrogen) or using the Direct-zol RNA mini-prep kit (Zymo Research #R2050). RNA libraries were prepared for sequencing with the TruSeq Stranded Total RNA with Ribo-Zero Gold kit using standard Illumina protocols with the exception that library amplifcation was performed using KAPA HiFi™ Real-Time PCR Library Amplification Kit (Kapa Biosystems #KK2701). Barcoded libraries (Illumina TruSeq) were loaded onto an Illumina HiSeq 1500 and single-end 100-base (SE100) sequencing was performed at the Advanced Genome Technologies Core Massively Parallel Sequencing Facility at UVM.