GSM4905009: 106C01; Schizosaccharomyces pombe; RNA-Seq
Temperature shifted cells were collected via vacuum filtration, flash frozen in liquid nitrogen, and pellets were stored at -80˚C. RNA was extracted using Phenol:Chloroform extraction as presented in Xu et al. Nature Methods 2019 Libraries were prepared according to manufacturer's protocols with NEBNext® Ultra™ II Directional RNA Library Prep Kit and NEBNext Poly(A) mRNA Magnetic Isolation kit,using 1ug total RNA input