GSM5766522: JH141_S52; Homo sapiens; ATAC-seq
GSM5766522
Viable cells were pelleted by centrifugation at 500g for 5 min at 4°C, then cell membranes were lysed in RSB with 0.1% Tween-20, 0.01% Digitonin, and 0.1% NP-40. Cell nuclei were pelleted by centrifugation at 500g for 10 min at 4°C, and digested with Tn5 for 30 minutes at 37°C. Transposition reaction was cleaned up with Qiagen PCR Clean-Up, and sequencing adapters added by PCR with KAPA HiFi enzyme mix . Final libraries were cleaned up with two 1.2x SPRI Select beads purifications and assayed by Qubit and TapeStation before submission for sequencing.