GSM5129177: K-Luc + mIL13BBz-CAR-T treatment [29567]; Mus musculus; RNA-Seq
le5 K-Luc tumor cells and Le6 mIL 13BBz-CAR+ T cells encapsulated into droplets, and libraries were prepared using Chromium Single Cell 3′ Reagent Kits v3 (1000075) according to manufacturer's protocol (10x Genomics). The generated scRNA-seq libraries were sequenced on a HiSeq using a read structure of 28 bp for Read 1, 8 bp for Index 1, and 101 bp for Read 2. Library preparation was performed using 10x Genomics Chromium Single Cell 3' Solution with v3 chemistry following manufacturer protocols (10x Genomics).