GSM4969269: A_PFC_HET_3676; Mus musculus; RNA-Seq
Adult male mice were deeply anesthetized with CO2, followed by cervical dislocation and decapitation. The brain was quickly dissected and immersed in ice-cold PBS. Brain regions of interest were isolated from 2-mm slices cut using a brain matrix. Samples were snap frozen and stored at -80 °C. Total RNA was isolated using Trizol Reagent (Thermo Fisher Scientific) according to manufacturer's recommendations. Total RNA was quality checked on an Agilent 2200 TapeStation system. RNA quantity was measured using a NanoDrop ND-1000 Spectrophotometer.Tissue collection and processing were performed by researchers blinded to the genotypes of the animals. In all experiments, wild-type littermates were used as controls. 200 ng RNA/sample was used for library preparation using TruSeq Stranded mRNA protocol (Illumina). The yield and quality of the amplified libraries was analysed using Qubit (Thermo Fisher) and the Agilent 2200 TapeStation system. The indexed cDNA libraries were normalised and combined, and the pools were sequenced on the Illumina Nextseq for a 75-cycle v2.5 sequencing run, generating 75 bp single-end reads.