GSM5706334: pENTER_VEC; Homo sapiens; ChIP-Seq
GSM5706334
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with Flag-antibody. DNA samples were end-repaired, A tailed, and adaptor ligated using TruSeq Nano DNA Sample Prep Kit (#FC-121-4002, Illumina), following the manufacturer's instructions. ~200-1500 bp fragments were size selected using AMPure XP beads. The final size of the library was confirmed by Agilent 2100 Bioanalyzer. The samples were diluted to a final concentration of 8 pM and cluster generation was performed on the Illumina cBot using HiSeq 3000/4000 PE Cluster Kit (#PE-410-1001, Illumina), following manufacturer's instructions. Sequencing was performed on Illumina HiSeq 4000 using HiSeq 3000/4000 SBS Kit (300 cycles) (#FC-410-1003, Illumina), according to the manufacturer's instructions.