GSM5830064: UREΔ/+;Tet2-/-_BS7; Mus musculus; Bisulfite-Seq
GSM5830064
Total bone marrow cells were isolated from the femurs, tibiae, pelvic bones and spines of mice and were treated with 1x ACK lysing buffer pH 7.4 to lyse the red blood cells. cKit+ Lymph- Ery- cells were  FACS-purified and gDNA was extracted using the Puregene Cell and Tissue kit (Qiagen) oxBS-seq library construction was performed using the Ovation Ultralow Methyl-Seq with TrueMethyl oxBS kit and the M01320 v10 manual (Nugen/Tecan)