WGS Illumina for Serratia ureilytica: Melopsittacus undulatus liver swab
Genomic DNA extracted with Promega Wizard Genomic DNA Purification Kit and stored at -20C. Extracted DNA quantified using a Quantus Fluorometer with QuantiFluor One dsDNA reagant. RNA/DNA ratios checked using the Nanodrop (v3.8.1) spectrophotometer (Thermo Fisher Scientific). Genomic DNA quality testing, library preparation using the Nextera XT DNA library kit v3.0 and Illumina short read sequencing of the extracted DNA conducted in accordance with the manufacturers instructions by the Australian Genome Research Facility. Reads filtered for quality scores >20 and adapters removed using the wrapper Trim Galore v0.4.4.
CM2008_163_i