16S rRNA V4 region amplified for a negative control sample taken through the DNA extraction process
The negative control was a sample tube in which no fecal material from a barn swallow was added, but which went through the entire DNA extraction process along with tubes with samples from barn swallows.  Then the V4 region of the 16S rRNA gene was amplified using the primers 515F and 806R.  Three independent PCRs were performed for each sample, which were then combined. Equimolar amounts of each sample were then pooled before sequencing on a 151 bp paired-end run of an Illumina MiSeq.
Hirundo rustica 293