GSM5592808: GRHL1-overexpressing_after_BMP_ActA_RNASeq [PREP0095_RHuan11781A_C02v1_11_RH_1024_S3]; Homo sapiens; RNA-Seq
Total RNA was prepared from Accutase-dissociated cells with an RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. RNA integrity was quantified with a TapeStation 4200 (Agilent). All RINe scores were ≥ 9.9. Libraries were constructed from total RNA using a Kapa mRNA HyperPrep Kit (Roche) by the Bauer Core at Harvard University.