GSM5236549: Sample_01_MEOX; Homo sapiens; RNA-Seq
RNA isolation of the FACS-purified GFP+ Treg cells was performed following the manufacturer's protocol using the Quick-RNA Microprep kit (Zymo Research). RNA quality control was performed with the Agilent 2200 Tape Station system and only RNAs having a RIN >8 were used for library preparation. Libraries for mRNA sequencing were prepared starting from 10 ng tot RNA for each sample by using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech-Takara)