GSM5735545: WT5Ba; Mus musculus; RNA-Seq
NucleoSpin RNA XS Kit (Macherey-Nagel, Bethlehem, PA) was used to purify RNAs from FACS-isolated mouse prostate basal, luminal and stromal cells. Reverse transcriptions were performed using SMART SeqTM v4 UltraTM Low Input RNA Kit for Sequencing (Clontech Laboratories, Mountain View, CA). CDNA libraries were prepared using Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA), and were sequenced using HiSeq 2500 sequencer.