DNA-Seq of Gordonia sp strain: EUFUS-Z928
Forlibraryconstruction,DNA/RNAisextractedfromasample.Afterperformingqualitycontrol(QC),qualifiedsamplesproceedtolibraryconstruction.ThesequencinglibraryispreparedbyrandomfragmentationoftheDNAorcDNAsample,followedby5'and3'adapterligation.Alternatively,"tagmentation"combinesthefragmentationandligationreactionsintoasinglestepthatgreatlyincreasestheefficiencyofthelibrarypreparationprocess.Adapter-ligatedfragmentsarethenPCRamplifiedandgelpurified.Forclustergeneration,thelibraryisloadedintoaflowcellwherefragmentsarecapturedonalawnofsurface-boundoligoscomplementarytothelibraryadapters.Eachfragmentisthenamplifiedintodistinct,clonalclustersthroughbridgeamplification.Whenclustergenerationiscomplete,thetemplatesarereadyforsequencing.
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