GSM4969021: P1_2n; Mus musculus; RNA-Seq
Cells from the left heart ventricle of mice at E16.5, P1 and P5 were dissociated by enzymatic dissociation and stained with a fixable viability stain prior to methanol fixation followed by rehydration. Fixed cardiac cells were stained with the cardiomyocyte marker MYH1 and the DNA marker Hoechst and diploid and tetraploid cardiomyocytes were isolated by FACS.   Sorted diploid and tetraploid cardiomyocytes were subjected to single cell RNA sequencing using the 10x Genomics platform. Single Cell 3' RNA-Seq libraries were prepared using Chromium Single Cell 3' Reagent Kits v2 (10x Genomics). NGS libraries were  constructed by adapter ligation and PCR mediated sample indexing (No. of cycles: 13).