GSM5943620: KO_2; Plasmodium berghei; RNA-Seq
GSM5943620
Total RNA was extracted from the 48% Nycodenz purified activated gametocytes (incubation at 25℃ for 15 min ) of the WT and Δpbgpr180 K1 parasites using the Qiagen RNeasy kit (Qiagen, Dusseldorf, Germany). The purity of RNA was checked using the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA). RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). A total of 1 µg total RNA was used to purify the mRNAs using poly-T oligo-attached magnetic beads, followed by fragmentation using divalent cations under elevated temperature in the first-strand synthesis reaction buffer. RNA libraries were prepared for sequencing using standard illumina protocols