GSM5945191: high_light_1; Klebsormidium nitens; RNA-Seq
GSM5945191
RNA extraction was performed using mechanical disruption of the frozen cell pellets in a Mini Bead Beater (Biospe Products) with a mixture of 2.7 mm glass beads for filament fragmentation and 0.5 mm glass beads for individual cell lysis (ratio 1/3) in the presence of an extraction buffer consisting of phenol:chloroform (1:1, v/v). Subsequently, RNA was purified using ISOLATE II RNA Plant Kit (Bioline) following manufacturer's instructions. RNA integrity number (RIN) was computed using an Agilent 2100 Bioanalyzer producing values greater than 8 per sample. Sequencing libraries were generated according to Illumina TruSeq Stranded mRNA protocol and sequenced on an Illumina NextSeq 500 sequencer producing approximately 17 million 50 nt long reads per sample.