Whole genome sequence of phosphate solubilizing bacterium, isolated from an agricultural plot treated with biocompost
Library preparation was performed using the SMRTbell template prep kit 1.0. SPv3 and SMRTbell barcoded adapter complete kit-96 (PacBio), following the Procedure and Checklist Preparing Multiplexed Microbial SMRTbell Libraries for the PacBio Sequel System (PacBio). Briefly, genomic DNA from overnight cell culture grown in standard nutrient broth (15 g/L of peptone, 3 g/L of yeast extract, 6 g/L of sodium chloride, 1 g/L glucose; pH 7.50.2) at 30 C was extracted using the Genomic-tip 20/G kit (Qiagen, Hilden, Germany) as recommended by the manufacturer. Genomic DNA was sheared to approximately 10 kb using g-TUBEs (Covaris Inc., Woburn, MA) and further processed according to the protocol.
InoP COM1