Paulownia fortunei
Genomic DNA was extracted from fresh leaves of Paulownia fortunei using a DNAsecure Plant Kit (Tiangen Biotech, Beijing, China). At least 10 g of sheared DNA was required to generate the 40-kb insert library. Preparation of the single-molecule real-time (SMRT) cell template involved DNA concentration, damage repair, end repair, ligation of hairpin adapters, and template purification. Subsequently, the genome was sequenced on the PacBio Sequel II platform (Pacific Biosciences, Menlo Park, CA, USA).
m64112_200320_001412