GSM6069386: ATAC-seq, primary RPE, biol rep2; Homo sapiens; ATAC-seq
GSM6069386
Fresh cells were washed with ice cold PBS containing protease inhibitor and nuclei were extracted prior to library construction. The standard ATAC-seq protocol was used, with the transposase reaction carried out for 30 minutes at 37C. 8-10 cycles of amplification were performed and dual size selection was performed with Ampure XP beads.