GSM5736058: siEmg1_m6AIP-seq_rep2; Mus musculus; OTHER
Total RNA was purified using TRIzol per the manufacturer's instruction. PolyA RNAs were purified using Dynabeads™ Oligo(dT)25 (Thermo, 61002). m6A IP was performed by using EpiMark® N6-Methyladenosine Enrichment Kit (NEB, E1610S). Libraries were constructed according to TruSeq Stranded mRNA Kit (Illumina) protocol. The concentrations for all the libraries were determined by KAPA Library Quantification Kit (KAPA Biosystems) following the manufacturer's protocol, and subjected to Next-generation high-throughput sequewncing using an illumina NextSeq 550 with a single-end 75-bp read length.