GSM5944579: ICR380-1-miRNA; Mus musculus; miRNA-Seq
GSM5944579
Cardiac samples from the remote zone of the left ventricle for RNASeq and miRNASeq were collected, frozen in liquid nitrogen and stored at −80 °C. The samples were then homogenized and RNA was isolated with TRIzol reagent. Cardiac samples from the left ventricle for RNASeq and miRNASeq were collected, frozen in liquid nitrogen and stored at −80 °C. The samples were then homogenized and RNA was isolated with TRIzol reagent. 3 days follow up samples. Library preparation was done using the Qiagen QIAseq miRNA Library Kit. 100ng of total RNA was converted into miRNA NGS libraries. Quality was analyzed using either Agilent Bioanalyzer 2100 or TapeStation 4200. The library pool(s) were then sequenced on Illumina NextSeq500. 21 days follow up samples. Library preparation was done using the Illumina TruSeq Small RNA Sample Prep kit. 100ng of total RNA was converted into miRNA NGS libraries. Quality was analyzed using either Agilent Bioanalyzer 2100 or TapeStation 4200. The library pool(s) were then sequenced on Illumina HiSeq2000 (2x100 cycles).