RNA-Seq of M13
Total bacterial DNA was extracted from the feces samples using the MagPure Soil DNA LQ Kit. The V3-V4 region of the 16S rRNA gene was amplified by polymerase chain reaction using universal primers 343F and 798R. The PCR products were extracted from agarose gels, purified twice using QIAxtractor SN002358, quantified by using Bioanalyzer 2100  and NanoDrop 2000, and sequencing analysis was performed by using Illumina HiSeq 2500.
M13