Fungal ITS
DNA was extracted using a DNeasy PowerSoil kit and protocol (Qiagen, Germantown, MD). DNA was amplified with barcoded primers targeting the ITS region of the fungal nuclear DNA. Amplification products were normalized using SequencePrep Normalization Plates (Life Technologies, Grand Island, NY) and sequenced with an Illumina MiSeq platform at the Molecular and Genomics Core Facility at the University of Mississippi Medical Center.
SS06