RNA-Seq of Leptobryum pyriforme: green gametophytes
mRNA was enriched from the total RNA by oligo (dT) magnetic beads. The purified mRNA was then broken into short fragments, which was used as template to synthesize the first- and second-strand cDNA. After reverse transcription, cDNA libraries were generated from appropriate size of DNA fragments using NEBNext Ultra RNA Library Prep Kit (NEB, USA).
UV-B_3