GSM5236533: PAO1_EX_2; Pseudomonas aeruginosa; OTHER
After in vivo RNA ligation by overexpression of T4 RNA ligase and cell lysis, total RNA were extracted and enriched for vfr-containing chimeric transcripts using sDNA probes specific to vfr mRNA. RNA libraries were prepared for sequencing using NEBNext Ultra II Directional RNA library kit