GSM5014310: MouseSpermONT; Mus musculus; RNA-Seq
Before RNA purification, sperm cells were lysed by 1% SDS and 0.1M DTT, with 10 min incubatin on 37 degree. Sperm RNAs were isolated using Trizol followed by acid phenol extraction. rRNA depletion was done for Illumina RNAseq. PacBio SMRTseq uses PacBio library construction protocol. Illumina sequencing uses standard RNAseq library construction protocol. Nanopore library was constructed using cDNA-PCR Sequencing Kit (SQK-PCS109, ONT) PacBio, Illumina RNAseq and Oxford Nanopore Technologies