12h_R1
Libraries for multiplex sequencing were prepared by the COS Deep Sequencing Core Facility. The Illumina TruSeq RNA Preparation Kit was used to purify mRNA from regeneration tips of the different time points. Size selection was performed on an E-gel (Bioanalyzer). Sample preparation was not stranded. Libraries of biological triplicates for each time point were sequenced single-end 100bp or 50bp on an Illumina HiSeq2000 sequencer.
12h_R1