GSM5733123: OCI-AML3 control-rep1; Homo sapiens; RNA-Seq
Total RNA was extracted from OCI-AML3 cells in control and treatment groups with Trizol according to the manufacturer's protocol. First-strand cDNA synthesis and cDNA libraries were constructed using the NEBNext® Ultra™ II RNA Library Prep Kit (New England Biolads). cDNA quality was determined on Agilent 2100 BioAnalyzer (Agilent Technologies), and then sequenced on Illumina novaseq 6000 to obtain pair-end 150 bp reads.