GSM4905195: Anti-PD-1 treated tumor from Hu-mice [T6]; Homo sapiens; RNA-Seq
RNA was isolated from tissue blocks using Zymo Direct-Zol RNA MiniPrep kit (Zymo Research). Total RNA subjected to ribosomal RNA depletion followed by library preparation using ScriptSeq Complete Gold Kit (Illumina). Libraries were quantified using KAPA Library Quant qPCR kit (Roche) and subjected to 75bp paired-end high output sequencing run on Illumina's NextSeq500.