GSM5058609: Left-over carotid artery Poldip2-floxed  Repeat 1; Mus musculus; RNA-Seq
Post sacrifice, the right and left carotid arteries were removed. The lumen of the carotid arteries were rapidly flushed using 29G insulin syringe containing 150uL Qiazol. The Qiazol flushings and the left-over artery samples were flash frozen in liquid Nitrogen. The RNAs were extracted using Qiagen's miREasy RNA extraction kit following standard protocol. RNA libraries were prepared for sequencing using standard Illumina protocols