K548R - Rep1 - Uncut fraction
Synthetic library was amplified with Illumina sequencing primers to install unique index sequence.  Single-end (83 cycles) Illumina sequencing was carried out on NextSeq platform using High-output flowcells with V2 or V2.5  chemistry.  Primer sequences and protocols are described in the manuscript.
20201206-AHTL2WBGXC-S9