GSM5013841: P14 Ileum WT + rmIl22 rep 2; Mus musculus; RNA-Seq
Ileum samples for mRNA analysis were collected in RNAlater (#76104, Qiagen) and kept at -80°C until RNA extraction. Total RNA was isolated using RNeasy Plus Mini Kit (#74134, Qiagen) and DNase Max Kit (#15200-50, Qiagen), followed by storage at -80°C. The quality of isolated RNA was determined using a Bioanalyzer (Agilent) with RIN values greater than 8. cDNA libraries were prepared using the TruSeq Stranded Total RNA Sample Preparation kit with Ribo Zero Gold (Rev. E; Illumina) according to manufacturer´s protocol.