GSM5670194: CAGE_AF7N_Pool_81_A2; Homo sapiens; RNA-Seq
Total RNA was isolated using RNeasy Plus Mini Kit (Qiagen) according to the manufacturer's protocol, and gDNA was eliminated by treatment with the RNase-Free DNase Set (Qiagen). CAGE libraries were prepared as described in (Takahashi et al, 2012, PMID: 22362160) with an input of 1.5mg of total RNA.