GSM6069382: ATAC-seq, iPSC-induced RPE, biol rep1; Homo sapiens; ATAC-seq
GSM6069382
Fresh cells were washed with ice cold PBS containing protease inhibitor and nuclei were extracted prior to library construction. The standard ATAC-seq protocol was used, with the transposase reaction carried out for 30 minutes at 37C. 8-10 cycles of amplification were performed and dual size selection was performed with Ampure XP beads.