GSM4785420: smallRNAseq_K562_wild_type-1; Homo sapiens; miRNA-Seq
RNA was isolated using the miRNeasy Mini kit (Qiagen) for small RNA seq. Total RNA was isolated from biological triplicates of each cell line using the miRNeasy Mini RNA isolation kit (Qiagen) including a DNase I digestion step to remove genomic DNA. Library preparation was performed following a previously published protocol (H. Kim et al., Bias-minimized quantification of microRNA reveals widespread alternative processing and 3' end modification. Nucleic Acids Res 47, 2630-2640 (2019)).