GSM6211447: EOC_45_S46; Bos taurus; RNA-Seq
GSM6211447
Total RNA was isolated from frozen explant endometrium (∼40 mg) by homogenizing in 1 mL of TRIzol reagent and a Qiagen RNeasy Mini Kit (Qiagen). RNA quantity and quality were determined using an Agilent Bioanalyzer (Agilent Technology). To eliminate DNA contamination, samples were treated with Dnase. RNA concentrations and integrity were determined by quantitative high-sensitivity RNA analysis on the Fragment Analyzer instrument (Catalog # DNF-472, Advanced Analytical Technologies, Inc., Ankeny, IA). RNA library preparation and sequencing was conducted by the University of Missouri DNA Core facility. Sequencing was performed on an Illumina NextSeq 500 sequencer to a depth of 30–40 million raw reads per sample.