GSM4785765: DEP-SiglecFhigh Neutrophil 2; Mus musculus; RNA-Seq
DEP exposed lungs were removed. Eosinophils, SiglecFlow and SiglecFhigh neutrophils were sorted. We extracted total RNA from samples using RNeasy Micro Kit (Qiagen), following the manufacturer's instructions, and treated DNaseI. We constructed RNA-seq libraries using the NEXTflex™ Rapid Directional RNA-Seq Kit (Perkin Elmer), following the manufacturer's instructions. In brief, we selected and fragmented poly-A-tailed mRNA ligated double-stranded cDNA fragments with adapters and amplified them for 9 cycles of PCR. We then performed RNA-seq using the Illumina HiSeq 2500 platform.