GSM5706082: WT-S Cayman3; Mus musculus; RNA-Seq
Hippocampi from unoperated or pump-implanted mice were dissected and immediately flash frozen on dry ice. Total RNA was extracted with TRIzol (Invitrogen) and chloroform (Invitrogen). RNA was then purified with the RNeasy mini kit (Qiagen) according to manufacturer's instructions. 400 ng of total RNA was used for library preparation. RNA libraries were prepared for sequencing using standard Illumina protocols