GSM4891473: AB6674; Homo sapiens; RNA-Seq
After informed consent (in accordance with Helsinki declaration), bone marrow aspiration was placed in EDTA-containing tubes (Beckton Dickenson), diluted 1:1 with ice cold FACS buffer (EDTA pH8.0 2mM, BSA 0.5% in PBS), placed on ice and immediately transported to the lab. Single-cell RNA-seq libraries were prepared as previously described {Jaitin, 2014}. In brief, mRNA from single cells sorted into capture plates were barcoded and converted into cDNA and then pooled using an automated pipeline. The pooled sample was linearly amplified by T7 in vitro transcription, and the resulting RNA was fragmented and converted into a sequencing-ready library by tagging the samples with pool barcodes and Illumina sequences during ligation, RT, and PCR. Each pool of cells was tested for library quality and concentration as described previously {Jaitin, 2014} single cell RNA-seq for gene expression quantitation