Illumina short-read sequencing of Archegozetes longisetosus: females mix of all life stages
Genomic DNA was isolated from ~500 mites susing QIAGEN Blood & Cell Culture DNA Mini Kit. Sequencing library was prepared with 1D Genomic DNA by Ligation Kit (Oxford Nanopore). Oxford Nanopore sequencing adapters were ligated using NEBNext Quick T4 DNA Ligase (NEB). The library was sequenced in-house on a MinION Spot-ON Flow Cell version R9.4.
Along_short