GSM5129561: AVPV_GDX_CRE1; Mus musculus; RNA-Seq
Brains from ovariectomized or E2-treated Cre+ mice were removed, flash frozen on dry ice, and micropunches of the AVPV brain region collected and pooled (n=4 brains per pooled sample). Pooled AVPV samples were run through the Ribotag immunoprecipitation pulldown procedure (Sanz et al., Current Protocols in Neuroscience, 88:e77, 2019) to isolate HA-tagged ribosomes from which RNA was extracted using the Qiagen RNeasy Plus Micro Kit (#74034) per the kit instructions. Unstranded mRNA library kits from Illumina that used polyA enrichment were used to create the library.