GSM6069067: sham spinal cord; Mus musculus; RNA-Seq
GSM6069067
An 8 mm segment of the spinal cord centered at the injury site was dissected and digested to obtain a single cell suspension. Cells were loaded onto the 10X Chromium Single Cell Platform (10X Genomics) at a concentration of 1,000 cells per μl (Single Cell 3′ library and Gel Bead Kit v.3) as described in the manufacturer's protocol. Generation of gel beads in emulsion (GEMs), barcoding, GEM-RT clean-up, complementary DNA amplification and library construction were all performed according to the manufacturer's protocol. Qubit was used for library quantification before pooling. The final library pool was sequenced on the Illumina Nova6000 instrument using 150-base-pair paired-end reads.