A3A deamination sequencing for 4mC in Deinococcus Radiodurans
We developed an A3A-mediated deamination sequencing (4mC-AMD-seq) method for genome-wide mapping of 4mC at single-base resolution. In the 4mC-AMD-seq method, cytosine and 5mC are deaminated by A3A protein to generate uracil and thymine, both of which are read as thymine in sequencing; while 4mC is resistant to deamination and therefore read as cytosine. Thus, the readouts of cytosines from sequencing could manifest the original 4mC sites in genomes.
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