RNA-seq of Human: A549 cells with PKR knockout
All cell lines were transfected at 70% confluence with 20 pmol (final siRNA used/well) of Dharmacon SMARTpool Brf-1 siRNA and or siRNA Universal Negative Control (Sigma) in serum-free Opti-MEM medium, using Lipofectamine RNAiMAX (Invitrogen). Cells were infected with MOI 0.1 VZV.
PKR_siBrf1_Infected_-33-R