Small RNA sequencing of jojoba: leaf
The RNA was extracted using the method previously described (Geng et al., 2008), and the small RNA sequencing libraries were constructed using TruSeq Small RNA Sample Prep Kits (Illumina Inc., San Diego, USA). The 18 ~ 30 nt length RNA was enriched, and T4 RNA ligase was used to add 3 and 5 adapter, and the adaptorligated RNA was reverse-transcribed and amplified by PCR to enrich the PCR product of length 140-160 bp to generate the library. The constructed library was sequenced using Illumina Hiseq2000/2500 (Illumina Inc., San Diego, USA), and the sequencing read length was single-ended 150bp
SCT_3_1