GSM4787210: IP-NEM_CovR_TAG_ATc200_Rep1; Streptococcus agalactiae; ChIP-Seq
CovR was immunoprecipitated with a Anti-FLAG monoclonal antibody coupled to magnetic beads for 2h at 4°C and samples treated with RNAse and Proteinase K overnight at 65°C prior to DNA purification for library construction. Libraries were prepared with Illumina TruSeq ChIP library preparation Kit (Illumina). Briefly, DNA was end-repaired and phosphorylated ends were treated to yield a protruding 3- 'A' base for ligation of Illumina's adapters. DNA was amplified by PCR with Illumina primers for 16 cycles and library fragments of ~350 bp were isolated from an agarose gel. Libraries were sequenced on an Illumina NextSeq 500/550 apparatus by single-end sequencing (75 bp).