mice-AMR evolution and tracking
All DNA samples were subjected to random shotgun sequencing. Briefly, 200 ng of DNA was used for metagenomic library preparation using the Westburg NGS DNA Library Prep Kit (cat. no. WB 9096). The genomic DNA was enzymatically fragmented for 12 min and DNA libraries were prepared without PCR amplification. The average insert size of libraries was 480bp. Prepared libraries were quantified using Qubit (Invitrogen) and quality checked on a Bioanalyzer instrument (Agilent). Sequencing was performed on NextSeq500 instrument using 2x150 bp read lengths.
M12day0MG