Metagenomic analysis of PAH and PCB contaminated Soil
Qubit quantified barcode ligated DNA samples were pooled at Equimolar concentration to attain 1 gpooled sample. Adapter ligation (BAM) was performed for 20 minutes using NEBnext Quick LigationModule (New England Biolabs, MA, USA). Library mix was cleaned up using 0.4X AmPure beads(Beckmann Coulter, USA) and finally sequencing library was eluted in 15 l of elution buffer and usedfor sequencing. (see below Figure 1: Overview of Library Preparation). Whole genome library wasprepared by using Native Barcoding kit (EXP-NBD103) from Oxford Nanopore Technology
MGB3