GSM4905143: KRAS_CD44v6pos_1; Homo sapiens; RNA-Seq
Cellular pellets were resuspended with TRIzol (Thermo Fisher cat. #15596026Ambion #15596018), and total RNA was extracted according to the manufacturer's instructions. Contaminating genomic DNA was removed by DNase (Qiagen cat. #79254) digestion. RNA quality and concentration were assed with the 2100 Bioanalyzer (Agilent cat. #G2939BA) and the Qubit fluorometer (Thermo Fisher cat. # Q33226), respectively. Total RNA-seq libraries were prepared using the Illumina TruSeq library kit (20020597). Libraries were sequenced as single reads of 50 bp with the Illumina HiSeq2500.