GSM5706501: DTCs, IAV_1; Homo sapiens; RNA-Seq
Total RNA was extracted 12 hours post infection from the H1N1(pdm09)-infected / non-infected DTC by Qiagen RNeasy Maxi Kit (Qiagen, Hilden, Germany), including on-column removal of DNA by digestion with rDNase for 15 min at room temperature, and 500 ng (each showing a RIN score from >9) were used for library preparation. After depletion of ribosomal RNA (QIAseq FastSelect RNA Removal Kit, Qiagen, Hilden, Germany) directional libraries were prepared with a NEBNext® UltraTM Directional RNA Library Prep Kit for Illumina® sequencing (#E7420S, New England Biolabs, Frankfurt, Germany).