GSM4968780: RNA-seq_Case10_B; Homo sapiens; RNA-Seq
Total RNA was extracted from tumor tissues using TRIzol™ Reagent (Life Technologies) for transcriptome analyses. RNA integrity, purity, and concentration were evaluated using an Agilent 2100 Bioanalyzer (Agilent Technologies). Nanodrop and Qubit 3.0. RNA-seq libraries were constructed using KAPA mRNA HyperPrep Kit. RNA libraries were prepared for sequencing using standard Illumina protocols