GSM4905260: +Serine/Glycine Replicate 4_From Donor 4; Homo sapiens; RNA-Seq
RNA was isolated using an Omega E.Z.N.A.® Total RNA Kit I The NEBNext Ultra II RNA Library Prep Kit (New England Biolabs) was used to generate TruSeq-barcoded RNA-Seq libraries. Libraries were quantified with the Qubit 2.0 (dsDNA HS kit; Thermo Fisher) and size distribution was measured with a Fragment Analyzer (Advanced Analytical) before pooling. A NextSeq500 (Illumina) was used for sequencing, and a minimum of 20 M single-end 75 bp reads per library were obtained.