GSM5830233: AOV_MSC_NMvOM_2wks_scRNAseq_InDrops; Homo sapiens; RNA-Seq
GSM5830233
VICs are extracted from diseased valves immediately after surgery. Endothelial layer is scraped off, then valve is minced into 1 cubic millimeter pieces then digested in 40 mL of 1mg/mL collagenase IA (Sigma) with gentle agitation in 37C CO2 incubator for 2 hours. Single cell suspension are then harvested and filtered through 100 um cell strainer, then resuspended then either cultured immendiately (MIX) or FACS-sorted (PUR) prior to culturing The cDNA library was prepared using the CEL-Seq/ MARS protocol with quality control analysis done with Agilent Bioanalyzer. samples are indexed (indexes: TATGCAGT, AGGCTTAG, AGAGGATA, ATAGAGAG, CTCCTTAC, and TACTCCTT), library construction is followed as per 10x Genomics protocol