GSM5014155: Miniature kidney organoids collected at differentiation day 8; Homo sapiens; RNA-Seq
Miature organoids were dissociated using cold active protease (protocol described in Ransick et al., 2019). Single cells were FACS sorted to select for DAPI- DRAQ5+ live cells, which were then profiled with a 10x Genomics Chromium Single Cell 3' GEM, Library & Gel Bead Kit (10X Genomics, PN-1000075). After recovery from the emulsion, cDNA was cleaned-up and amplified by PCR, examined on a 4200 Tape station (Agilent) for yield assessment, and then processed into barcoded library for Illumina sequencing. Paired-end sequencing on the Illumina NextSeq 500 platform was performed using the NextSeq® 500/550 High Output Kit v2 (FC-404-2002) and the 150-cycle flow cell (FC-410-1002). scRNA-seq