GSM5013673: TLR296-P2; Homo sapiens; RNA-Seq
RNA was isolated using the RNeasy Mini Kit (Qiagen) and processed as per manufacturer's instructions. Strand-specific RNA-seq libraries were prepared using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, UK), as per manufacturer's instructions. Sequencing was performed on an Illumina NovaSeq 6000 (Illumina, USA) to generate 150 bp paired-end reads. For patient samples, coding and long non-coding RNA was sequenced, whereas only the coding mRNA was sequenced for all other samples.