GSM5739150: WT_SPG_2; Mus musculus; RNA-Seq
GSM5739150
Spermatogenic cells were collected with Fluorescence activated Cell Sorting (FACS) and Spermatocytes were flash frozen on dry ice, and RNA was harvested using Trizol reagent. Sequencing libraries were constructed from 500 pg of amplified cDNA using TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme, TD503) according to manufacturer's instructions. Barcoded libraries were pooled and sequenced on the Illumina HiSeq X Ten platform with 150 bp paired-end reads