m6A-Seq and RNA-Seq analyses of female and male mice livers
300ug total RNA isolated from frozen livers with TRIzol and depleted of rRNA with RiboMinus (Life Technologies). mRNA was purified from each sample using Oligo(dT) Dynabeads (Invitrogen) and subjected to first strand cDNA synthesis and library prep using TruSeq stranded mRNA library prep kit (Illumina)and sequenced on an Illumina HiSeq 3000 with single-end 50-bp read length.
PTTSmmMettl14_WT_F.rep8