GSM5799927: KPF CAF-HIF2 KO rep3; Mus musculus; RNA-Seq
GSM5799927
Tumors were harvested and single-cell suspensions were made by a combination of mincing, enzymatic digestion (Liberase and Accutase), and straining. Chromium Single Cell 3' Library & Gel Bead kit v2 (10X Genomics, Cat#PN-120237) and the 10X Genomics Chromium instrument were used to generate gel beads in emulsion and single-cell cDNA libraries, with 5,000 cells loaded per channel. Single-cell cDNA libraries were prepared and sequenced using standard 10X Genomics and Illumina protocols.