GSM5706141: Kras_tumor_rep3; Mus musculus; ATAC-seq
For colonic epithelium, distal colon was scraped using a razor blade to enrich for colonic epithelium. For colonic tumor, animals were sacrified when they became moribund. Tumor lawns along colon were resected or scraped using a razor blade. Harvested tissues were flash frozen in liquid nitrogen and stored at -80ºC. Nuclei isolation was performed using ActiveMotif ATAC-Seq kit following manufacturer's protocol. 100,000 nuclei was used for ATAC-Seq library preparation. ATAC-Seq library was prepared using ActiveMotif ATAC-Seq kit following the manufacturer's instructions.