WGS metagenomic sequencing of rectum fecal sample fromcat D001, collected with minearal lubricant
Fecal DNA samples were extracted using the Qiagen Allprep PowerFecal DNA/RNA kit (QIAGEN, MD). The homogenization step was conducted using the Qiagen PowerLyzer24 instrument (QIAGEN, MD). 1.5-2 g of genome DNA was fragmented by M220 Focused-ultrasonicator with a 500bp targeted insert size (Covaris, MA). Metagenomic sequencing libraries were made using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, MA), following the manufacture protocols. The size range of the final library insert size was 500-600 bp. The libraries were sequenced on an Illumina NovaSeq6000 sequencing machine with 150-bp paired-end reads at the Genomics Service Laboratory at the HudsonAlpha Institute for Biotechnology.
D001_fecal_miOil_WGSmetagenomics