ncRNA-seq of Japanese flounder:spleen
The RNA molecules in a size range of 1830 nt were enriched by polyacrylamide gel electrophoresis (PAGE). Then the 3 adapters and 5 adapters were ligated. The ligation products were reverse transcribed by PCR, and the 140-160 bp PCR products were enriched to generate a cDNA library, which was sequenced using Illumina HiSeqTM 2500
A018