GSM4970382: sgRNA; synthetic construct; OTHER
Cells were pelleted and genomic DNA was extracted using the Gentra Puregene Cell Kit from QIAGEN according to manufacturer's instructions. The region of the sgRNA lentiviral construct containing the sgRNA sequence was PCR-amplified from genomic DNA using primers containing Illumina P5 and P7 adapters. Product size was confirmed via gel electrophoresis. PCR products were cleaned up using AMPure XP beads. Library concentrations were determined with a NanoDrop.