GSM4968734: Case5_T; Homo sapiens; OTHER
Purity of the extracted DNA was assessed by electrophoresis of the DNA through a 1% agarose gel, and DNA concentration was quantified with a Qubit Fluorometer using the QubitTM dsDNA HS Assay Kit(Themo Fisher Scientific)Kit. Ten paired DNA libraries were constructed using Agilent 60M sample preparation kit following the manufacturer's instructions and separately sequenced on Illumina NextSeq500 with 2x150 bp. Adaptor sequences were trimmed and reads were processed.