GSM5600322: zebrafish day 0; Danio rerio; RNA-Seq
GSM5600322
Cells were resuspended in PBS containing 0.04% BSA at a concentration of 2600 cells/ 33.8 μL solution. In total, 2600 cells per sample were loaded into each well of a 10X Chromium single cell capture chip with a recovery of 272 cells for uninjured heart, 141 cells for 3 days post injury and 368 cells for 7 days post injury sample. The captured cells then underwent lysis, reverse transcription, cDNA amplification, and library preparation with indexing per the manufacturer's protocol (10X Genomics). The libraries were sequenced together on an Illumina HiSeq 4000 (Illumina Inc., San Diego, CA) using a high output 150 cycle kit with read lengths recommended by 10X Genomics.