GSM6213150: 858; Homo sapiens; RNA-Seq
GSM6213150
Total RNA was isolated from whole blood, stored in paxgene, using the ExtractionMonarch® Total  RNA Miniprep Kit (NEB #T2010). Upon blood isolation, Monarch DNA/RNA Protection Reagent  (supplied as a 2x concentrate) was added undiluted to an equal volume of blood. Addition of the  protection reagent and the following RNA isolation was performed as described in the Kit's manual  for Total RNA Purification from Mammalian Whole Blood Samples. After quantification on a NanoDrop ND-1000 (Thermofisher) and Bioanalyzer RNA 6000 Nano  assay (Agilent), 140-300ng of total RNA from samples passing quality control were processed  using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD (Lexogen, 015.96) for library  preparation. Libraries were assessed for molarity and median library size using Bioanalyzer High  Sensitivity DNA Analysis (Agilent, 5067-4626)