GSM5733043: Injured nerve input1; Rattus norvegicus; ChIP-Seq
Freshly dissected rat sciatic nerve was used for ChIP-seq using the MNase protocol described previously (Ma et al., 2018) with 4μg of JUN antibody (Santa Cruz SC-1694, RRID:AB_631263). Libraries were prepared from ChIP DNA, and respective inputs, using the Illumina TruSeq ChIP Sample Preparation Kit. Two biological replicates were performed, and samples with respective inputs were sequenced on a Illumina HiSeq 2500 instrument at the UW Biotechnology Center.