GSM5235904: Nonclassical monocytes LAIRKO5 [SAM24362401]; Mus musculus; RNA-Seq
Non-classical monocytes were sorted (purity 98%) from the bone marrow of LAIR1−/− and LAIR1+/+. 5000-10000 cells were collected and RNA was extracted using Nucleospin RNA XS (Takara) and low input RNA sequencing was performed (Clontech). RNA libraries were prepared for sequencing using standard Illumina protocols