GSM5800046: ChIP-Seq Newborn erythroblasts HIC2; Homo sapiens; ChIP-Seq
Cells were crosslinked with 1% formaldehyde for 10 minutes at room temperature. Fixed cells were lysised in 1mL Cell Lysis Buffer (10mM Tris-HCl pH 8, 10mM NaCl, 0.2% NP-40/Igepal). Nuclei were collected and lysised in 1mL Nuclear Lysis Buffer (50mM Tris-HCl pH 8, 10mM EDTA pH 8, 1% SDS). Chromatin was sonicated at 4 degrees C (Epishear, Active Motif). Chromatin was incubated with antibody and then decrosslink at 65C overnight. Samples were processed for library construction for Illumina sequencing using Illumina's TruSeq ChIP Sample Preparation Kit (Illumina cat# IP-202-1012) according to Illumina's instructions..