GSM4551552: Sample 1 WT; Mus musculus; RNA-Seq
Adult Wildtype hippocampal single cell suspensions were obtained by either mechanical dissociation at 4°C or 30 minutes enzymatic digestion at 37°C and subsequent percoll-gradient centrifugation for debris and myealin removal. The sample loaded onto the 10x genomics chip is a pool of hippocampal cells from 3 adult mice. Library construction was performed according to the 10x Chromium Single Cell 3' Reagent Kit v3.