GSM5627526: H; unclassified Stomphastis; RNA-Seq
GSM5627526
Twenty larvae with and without oblivious sign of disease were preserved in an RNA stabilization reagent (RNAprotect®, QIAGEN Cat No.:76104) for further RNA extraction and sequencing. The whole larvae were transferred to Qiazol lysis reagent for RNA extraction according to the manufacturer's instructions (QIAGEN; Cat No.: 79306). The RNA samples were treated with DNase I for 1 h at 37°C and then their concentrations were measured using a spectrophotometer and integrity was ensured through analysis of RNA on a 1% (w/v) agarose gel. After checking the RNA quality, total RNA from two samples (infected and uninfected) were submitted to the Genewiz sequencing facility (Jiangsu, China) for library preparation (after eukaryote ribosomal RNA depletion) and strand specific total RNA sequencing on NovaSeq platform.