ddRAD Dascyllus abudafur: Madagascar
Double digest restriction associated DNA (ddRAD) libraries were prepared using 500 ng of high quality DNA per sample following the protocol described by Peterson et al., (2012) with some modifications. Briefly, the genomic DNA is digested at 37C for three hours using the restriction enzymes SphI and MluCI (NEB) followed by a ligation step, where each sample is assigned to one of sixteen unique adaptors.
ddRAD next gen seq Dascyllus abudafur 114-121