GSM5737371: siNTRK2_Sen_2; Homo sapiens; RNA-Seq
RNA was extracted with RLT buffer (Qiagen) and purified using the QIAcube system (Qiagen) through the RNeasy plus method. The quality and quantity of RNA were checked using Agilent RNA 6000 nano kit on the Agilent Bioanalyzer. 125ng of high quality of RNA was used for sequencing library prep using Illumina TruSeq Stranded mRNA Library prep kit according to manufacturer's protocol (Illumina, San Diego, CA). The quality and quantity of sequencing libraries were checked using Agilent DNA 1000 Screen Tape on the Agilent Tapestation.