10X scRNA-Seq of human PBMC- totalseq hashtag
Pooled cells were resuspended in ice cold PBS with 0.04% BSA in a final concentration of 1800 cells/uL. Single cell suspensions were then immediately loaded on the 10X Genomics Chromium Controller with a loading target of 26,000 cells. Libraries were generated using the Chromium Next Gem Single Cell 5 Reagent Kit v2 (Dual Index) per manufacturers instructions with additional steps for the amplification of HTO barcodes and V(D)J libraries (10X Genomics, Pleasanton CA). Libraries were sequenced on Illumina NovaSeq with a sequencing target of 30,000 reads per cell RNA library, 5,000 reads per cell HTO barcode library, and 5,000 reads per cell for V(D)J libraries.
Vaccine_post_surface_10X