GSM4970032: std2_liv_frac_neg; Mus musculus; RNA-Seq
RNA was isolated using microRNA isolation kit (Qiagen) according to manufacturer's instructions. The library preparation and sequencing were performed at the Genome Technology Access Center (GTAC) at Washington University School of Medicine. Briefly, the total cellular RNA was also prepared for RNA sequencing with the Clonetech SMARTer kit according to manufacturer's protocols, ligated with adapters and unique molecular indexes for each sample for every read, and then sequenced on one single-end 50bp lane on an Illumina HiSeq 3000.