GSM5238534: TBBPA.DBPE_3_FRC_Rep3; Danio rerio; RNA-Seq
Following exposure, 48-hpf whole embryos were homogenized using RNAzol (Molecular Research Center, Inc).  RNA was purified using the Direct-zol MiniPrep kit (Zymo Research) and included the optional in-column DNase 1 digestion treatment for 15 minutes 100 bp sequencing was conducted using an Illumina HiSeq 3000 sequencer mRNAs were enriched by polyA selection. Libraries were prepared with the PrepX™ mRNA and Illumina sequencing workflow (Wafergen Biosystems)