GSM5609968: DIPG17_WT_H3K27Ac_rep2; Homo sapiens; OTHER
GSM5609968
CUT&RUN was performed as previously described (Skene and Henikoff, 2017) with minor modifications. Total RNA samples were purified the same as for RT-PCR. RNA-seq libraries were prepared and deep sequencing was performed by Columbia University Genome Center. CUT&RUN libraries were prepared using ACCEL-NGSTM 1S plus DNA library kit and library PCR was performed using standard Illumina Nextera Dual Indexing primers. Samples were pooled and sequenced using paired-end sequencing on Illumina NextSeq 500 platforms at Columbia University Genome Center.