GSM6212215: Primary myotube_PA32h_i3; Homo sapiens; ChIP-Seq
GSM6212215
Myotubes were cross-linked in 1% formaldehyde and lyzed in SDS buffer. Nuclei were pelleted by centriguation and sonicated to an average length of 200-500 bp. 2-10 μg DNA was combined with 2.5 μg antibody for H3K27ac (Ab4729) and immunoprecipitated with Protein G Sepharose beads. Chromatin was de-cross-linked in 1% SDS and 0.1 M NaHCO3 and DNA was purified using Qiagen MinElute PCR purification kit. 3-10 ng of immunoprecipitated DNA was used to generate adapter-ligated DNA libraries using the NEBNext® Ultra DNA library kit for Illumina (New England Biolabs, E7370L) and indexed multiplex primers for Illumina sequencing (New England Biolabs, E7335)