GSM5944539: 1075F-RNA; Mus musculus; RNA-Seq
GSM5944539
Cardiac samples from the remote zone of the left ventricle for RNASeq and miRNASeq were collected, frozen in liquid nitrogen and stored at −80 °C. The samples were then homogenized and RNA was isolated with TRIzol reagent. 3 days follow up samples.  Library preparation was done using Illumina TruSeq stranded total RNA sample Library Prep Kit with the starting material of 500 ng total RNA. Library quality was inspected on Agilent Bioanalyzer 2100 or BioAnalyzer 4200 TapeStation. The library pool was quantified using qPCR and sequenced on Illumina NextSeq550 (2x75 cycles). 21 days follow up samples.  Library preparation was done using Illumina ScriptSeqV2 Library Prep Kit with the starting material of 500 ng total RNA. Library quality was inspected on Agilent Bioanalyzer 2100 or BioAnalyzer 4200 TapeStation. The library pool was quantified using qPCR and sequenced on Illumina HiSeq 2000 instrument (2x100 cycles).