Neandertal Mez3 not repaired DNA library sequenced on Illumina HiSeq 2500 with with Rapid SBS Kit v2 (180620) and TruSeq SBS Kit v3 (181219), paired end mode
DNA extraction was performed using 120 mg of tooth powder. Extracted DNA were converted into single-stranded DNA libraries (Gansauge et al., 2017). We didn't performed any DNA treatment before library preparation. The library was amplified with AccuPrime Pfx DNA polymerase (Invitrogen, USA) by using two library-specific uniquely barcoded primers. The PCR product was purified using the MinElute PCR purification kit (QIAGEN, Germany) and then size-selected on a 2% E-gel (Thermo Fisher Scientific, USA). DNA fragment between 155 and 250 bp purified form the gel using the QIAquick Gel Extraction Kit (QIAGEN). Library validation was performed using an Agilent 2100 Bioanalyzer with DNA High Sensitivity chip (Agilent, USA) and quantified via qPCR using a KAPA Library Quantification Illumina Kit protocol (KAPA Biosystems, USA).
L01000625_PE