GSM5830366: MIA PaCa-2 TAMs rep3; Homo sapiens; RNA-Seq
GSM5830366
Total RNA was extracted using RNeasy Micro Kit (Cat#74004, Qiagen)following the manufacturer's instructions and checked for a RIN number to inspect RNA integrity by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US).Qualified total RNA was further purified by RNAClean XP Kit (Cat A63987, Beckman Coulter,Inc.Kraemer Boulevard Brea, CA,USA)and RNase-Free DNase Set  (Cat#79254, QIAGEN, GmBH, Germany). RNA libraries were conducted with standard Illumina protocols, using VAHTS Stranded mRNA-seq Library Prep Kit for Illumina® (Vazyme, China), Agencourt ® AMPure XP Beads(Beckman, USA),Qubit™ dsDNA HS Assay Kit(Invitrogen, USA) and Agilent High Sensitivity DNA Kit(Agilent, USA).