NI9 Acetate
Three independent cultures of M. hydrocarbonoclasticus NI9 in a media containing 10% M9 minimal media, 40% Seawater, and 50% distilled water with Jet-A or 0.2% Sodium acetate as the carbon source. Cells were grown up to mid log phase (0.4 OD 600). The total RNA was extracted from cells using Qiagen Total RNA kit (Qiagen, Germantown, MD) as described by the manufacturer. Ribosomal RNA was removed from total RNA using magnetic beads (Ribo-Zero magnetic kit (catalog no. MRZB12424; Epicentre) and the ribosomal RNA depleted total RNA was used for library preparation.  NEBNext Ultra Directional RNA Library Prep Kit (New England BioLabs, Ipswich, MA) was used for library preparation, which used dUTP in cDNA synthesis to maintain strand specificity.
TG Acetate