GSM5740156: 324IFN; Mus musculus; RNA-Seq
GSM5740156
RNA from the sorted cells was isolated using the RNeasy Plus Micro kit (QIAGEN). Quality control was performed by bioanalyzer (Agilent), and RNA samples with an RNA integrity number (RIN) > 8 were processed for library preparation using SMARTer Ultra Low Input reagent (Takara) and Nextera XT DNA (Illumina) library preparation kits Libraries were sequenced with paired-end reads of 126bp on a HiSeq2500 sequencer (Illumina) to reach 50 million read pairs per sample. For each group, IFN or vehicle, and genotype, data are derived from three distinct mice, with separate processing from cell sorting to sequencing.