16S rRNA amplicon sequencing of Scylla serrata gut
Purified PCR products (QIAGEN Gel Purification Kit (QIAGEN, Germany)) from each sample were end-repaired and dA tailing using NEBNext Ultra II End Repair/dA-Tailing Module (New England Biolabs) was performed according to the protocol described by the manufacturer. The dA tailed PCR products were ligated with barcode adaptors using the Oxford Nanopore Native Barcode kit (EXP-PBC096) and the Oxford Nanopore 1D Ligation Sequencing kit (SQK-LSK109).
EW2C2