GSM5919520: 34LG; Mus musculus; RNA-Seq
GSM5919520
The following morning, both mice were removed together and euthanized one at a time in a randomly selected order via urethane injection. Mice were rapidly dissected and left kidney, right adrenal gland, left lung, left and right atria, and left and right ventricle were all independently flash frozen in either RLT Plus buffer (kidney, adrenal gland, lung) or Trizol (all heart tissues) and stored at -80C degrees. Once all tissues had been collected from all three pairs of mice, tissues were removed from -80C and homogenized. RNA was extracted one tissue at a time using RNeasy Plus Mini kit (Qiagen, 74134) in a Qiagen Qiacube for all samples stored in RLT buffer and by hand using RNeasy plus mini kit with Qiagen Dnase treatment (RNase-Free DNase Set, cat#79254). Libraries were constructed using the TruSeq RNA Library Preparation Kit v2, Set B (Illumina, RS-122-2002) All samples for each organ were pooled and each organ run across one lane of a hiSeq4000