GSM4969861: SS; Mus musculus; RNA-Seq
At 8hrs cells were washed once with PBS, and lifted into suspension by incubating with Accutase for 5mins at 37C. Cells were labeled with TotalSeqB hashtag antibodies and pooled for 10x capture and sequencing. Chromium Single Cell 3' GEX Version 3.1 for RNA library, and Chromium Single Cell 3ʹ Feature Barcode Library Kit for tag libraries.