Whole-genome resequencing of Oncorhynchus mykiss: adult male fin
Individuals were barcoded; Chelex DNA extraction; Individual sample DNA concentrations were quantified and normalized with pico-green fluorescence on a Tecan M200; Fragmented with NEBNext double-stranded DNA (dsDNA) fragmentase randomly throughout the genome; Fragments of 400-600 base pairs (bp) were size-selected; Ends of DNA strands were repaired with NEBNext end prep and cleaned with SpriSelect Beads; Quantity and quality of each library was assessed using quantitative PCR and a Tape Station with a DNA High Sensitivity D1000 kit; Final libraries were normalized and sequenced targeting 500 million paired-end reads per library.