double-8_L2_A008
Briefly, total RNA was extracted from 2-week-old seedlings grown on 1/2 MS medium with Trizol reagent (Invitrogen) in 2 biological replicates. 30 g RNA was loaded on 15% urea-PAGE gel, and the small RNAs range from 18-30 nt were sliced from the gel. Small RNAs were recovered by soaking the smashed gel in 0.3M NaCl overnight, followed by precipitation with ethanol. Small RNA libraries were constructed following instructions from NEBNextR Small RNA library Prep Set for IlluminaR(NEB, E7300S).