GSM5920951: L1_4W_Rep2; Mus musculus; RNA-Seq
Flow citometry was used to sort tomato positive epidermal cells. Full length mini bulk RNA-seq was performed using a modified version of the Smart-seq2 protocol. Briefly, the Smart-Seq2 biotinylated Oligo(dT) was bound to Dynabeads MyOne Streptavidin T1 beads (Thermo-Fisher) and used to isolate mRNA from the cell lysate. Reverse transcription, amplification and library preparation were performed as for the single cell library preparation doubling the volumes.