GSM4905556: KO_Tumor_4; Mus musculus; RNA-Seq
RNA was extracted from snap frozen tumor and skin sample stored in RNAlater solution . Total RNA was isolated using RNeasy Mini Kits (Qiagen). Total RNA from mouse skin was used to create ribosomal RNA-depleted cDNA libraries (NEBNext® Ultra™ RNA Library Prep Kit). Each sample was sequenced to 40 million raw reads in a single end 50bp sequencing run on the Illumina Hiseq3000 or Novaseq6000.