AVENIO Expanded on synthetic human reference standard: Horizon  Discover 100% Multiplex I Wild Type cfDNA Reference Standard, replicate 2
DNA was prepared into libraries using  the Roche AVENIO expanded kit according to manufacturer's recommendations.  Library was sequenced on Illumina NextSeq 500  and analyzed on the AVENIO Oncology Analysis Server. Deduped bam used  for SNV and INDEL calls. Sorted bam used for CNV and fusion calls.