AVENIO Expanded on human cfDNA  mixture: 0.1% AF spike-in
DNA was  prepared into libraries using the Roche AVENIO expanded kit according to  manufacturer's recommendations. Library was sequenced on Illumina NextSeq  500 and analyzed on the AVENIO  Oncology Analysis Server. Deduped bam used for SNV and INDEL calls. Sorted  bam used for CNV and fusion calls.