ATAC-Seq of R562 SMC from ascending aorta
For bulk ATAC-seq, sorted SMCs were processed according to the Omni-ATAC-seq protocol. In brief, freshly sorted SMCs were lysed in cold ATAC-seq resuspension buffer containing 0.1% NP40 (11332473001, Sigma), 0.1% Tween-20 (11332465001, Sigma) and 0.01% Digitonin (G9441, Promega) for 3 min, washed and resuspended in transposition mix (20034198, Illumina). After 30 min of reaction in a 37C thermomixer, tagmented DNA were cleaned up using a Zymo DNA Clean and Concentrator kit (D4014, Zymo Research) and amplified by NEBNext High-Fidelity 2x PCR MasterMix (M0541L, New England Biolabs). We used adapter sequence publised previously. Numbers of amplification cycles were optimized by qPCR and a range of 6 to 10 cycles were applied to 3,000 to 8,000 sorted SMCs. Amplified libraries were cleaned up by Zymo DNA Clean and Concentrator kit (D4014, Zymo Research) and sequenced on a NovaSeq 6000 (Novogene).