rDNA metabarcoding of bovine gastrointestinal nematode: L3 stage
A 350 bp fragment encompassing the rDNA ITS-2 was PCR-amplified, then a second round limited cycle PCR was performed to add combinatorial barcoding adaptors to allow amplicons from many populations to be pooled and then sequenced on an Illumina MiSeq Sequencer using a 500-cycle pair-end reagent kit