Illumina NovaSeq 6000 paired end sequencing; scRNA-seq of human trophoblast stem cells as a model of trophoblast-cytotrophoblast (TB-CTB)
scRNA-seq of human trophoblast stem cells as a model of trophoblast-cytotrophoblast (TB-CTB)
Placenta derived Okae et al. human trophoblast stem cells cultured in Okae et al. conditions (ttps://doi.org/10.1016/j.stem.2017.11.004). In addition, we sequenced SHEF6 primed human pluripotent stem cells as a control.  Individual cells were manually isolated with a mouth pipette and subjected to single-cell full-lengths transcriptome profiling using a modified version of Smart-Seq2. For each sample, RNA separation was performed using biotinylated oligo-dT30VN-tailed oligonucleotides (IDT) conjugated to Dynabeads Streptavidin C1 (65001, Invitrogen) in an RNAse-inhibitor (RNAsin; N2615, Promega) supplemented buffer solution.   Sequencing libraries were prepared according to an adapted version of the G&T protocol previously described.