GSM5800201: HTG_P_4; Homo sapiens; RNA-Seq
For each sample, trizol (TAKARA BIO INC., CA, Japan) was added into the whole blood immediately after the blood samples were collected. The ratio of trizol to whole blood is 3:1. And total RNA was isolated and purified using total RNA extraction kit.The chain-specific library was constructed by removing the ribosomal RNA. RNA libraries were prepared for sequencing using standard Illumina protocols