Different tissues of a fly larva individual, biological replicate 2, sequencing run 1
Fly were dissected by different tissue/organ types, and the barcoding sequences were amplified with unique molecular identifiers (UMIs) and sample index by customized PCR protocol. Pooled PCR products of the 3kb readout sequence were used for SMRTbell library preparation (Sequel platform), which follows the standard protocol of SMRT sequencing. CCS reads were generated by pbccs 3.4.1.