WGS of Mycolicibacterium smegmatis mc2 155 knock-out mutant MSMEG_6302 (LigC1), MSMEG_6304 (LigC2) and MSMEG_6301(Prim2) C
The sequencing libraries were prepared, using the Vazyme TruePrep DNA Library Prep Kit V2 for Illumina preparation protocol (Vazyme Biotech Co.,Ltd, Nanjing, China). Whole-genome shotgun sequencing was performed on a NextSeq 500 platform, at a read length of 2 x 150 bp (300 cycles).