GSM6069529: CTRL-100p00METH (bs-seq); Homo sapiens; Bisulfite-Seq
Described in manufacturer's data sheet Libraries were prepared as outlined in the methods section. Briefly, DNA was bisulfite converted, and five DNA fragments, representing promoter regions of five established tumour suppressor genes, were amplified using custom set of primers (MLH1, chr3:36993123-36993500; CDKN2A, chr9:21974554-21974921; MGMT, chr10:129467118-129467477; CDH1, chr16:68737102-68737469; BRCA1, chr17:43125171-43125550), indexed, and sequenced on the Illumina MiSeq following the manufacturer's protocols.