Illumina HiSeq 4000 sequencing; TNFR2 induced priming of the inflammasome leads to a RIPK1-dependent cell death in the absence of XIAP
TNFR2 induced priming of the inflammasome leads to a RIPK1-dependent cell death in the absence of XIAP
Genotypes: X = xiap-/- mice (MGI:3629213); TR1X = xiap-/-tnfr1-/- mice (MGI:3629213, MGI:1861040); Bone marrow derived macrophages were plated at 1 x 10 ^6 cells/ml and stimulated with 100 ng/mL of TNFR2-specific ligand (TNC-TNF) for 2 h.  RNA was extracted using GENEzol Reagent (Geneaid) and cleaned using PureLink RNA Mini Kit (Invitrogen) according to the manufacturer's instructions.  150ng of total RNA were used for Poly A library preparation. Strand specific cDNA libraries were prepared.