Methylome capture of HG006 using SPlinted Ligation Adapter Tagging (SPLAT)
100 ng gDNA was fragmented to 400 bp (Covaris). Bisulfite conversion was performed using the EZ DNA Methylation-Gold kit (Zymo Research). SPLAT libraries were constructed as described previously (Raine et al., 2017). The libraries were amplified with 4 PCR cycles using KAPA HiFi Uracil+ PCR enzyme (Roche).