GSM5133343: U20S_siCtrl_RNA-seq_3; Homo sapiens; RNA-Seq
RNA extraction was performed using Qiagen Rneasy Plus mini kit. NGS library prep was performed with Illumina's TruSeq stranded mRNA LT Sample Prep Kit following TruSeq Stranded mRNA Reference Guide (Oct.2017) (Document # 1000000040498v00). Libraries were prepared with a starting amount of 1000 ng and amplified in 10 PCR cycles. Libraries were profiled in a High Sensitivity DNA on a 2100 Bioanalyzer (Agilent Technologies) and quantified using the Qubit dsDNA HS Assay Kit, in a Qubit 2.0 Fluorometer (Life Technologies).