GSM4970511: MTO54 p14 +6 d4 replicate 1; Mus musculus; RNA-Seq
Organoids were lyzed with Trizol and RNA was purified using PureLink® RNA Mini Kit RNA libraries were prepared for sequencing using the kits NEBNext® Poly(A) mRNA Magnetic Isolation Module (Cat# E7490) and NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (Cat#E7770), following manufacturer´s instructions and 9-10 cycles of amplification.