GSM6285658: 1 % INPUT Control (transfected with scrambled siRNA) -Dex; Homo sapiens; ChIP-Seq
Chromatin immunoprecipitation (ChIP) assays were performed as previously described. Soluble chromatin was prepared using MNase digestion [50mM Tris-HCl, pH 7.5, 4mM MgCl2, 1mM CaCl2, 0.32mM sucrose, 2mM NaF, 0.2mM NaVan] with 2 units of MNase (Sigma, UK) /180μg chromatin, then immunoprecipitated with H300 GR antibody (Santa Cruz; sc-8992), RNA Polymerase II (phosphoserine-5) antibody (ACTIVE MOTIF; 39233) or rabbit non-immune serum (Santa Cruz; sc-2027). For sequencing, libraries were prepared from two replicates/condition. NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®