Single cell RNA-seq of RH5 vaccine-specific IgG+ B cells: 2-weeks after final vaccination (monthly boosting)
Sequencing libraries were created using the Nextera Index Kit v2 (FC-131-2001, Illumina) and the Nextera XT DNA Sample Preparation Kit (FC-131-1096, Illumina). Libraries were then purified with AMPure XP beads (A63881, Beckman Coulter) and quantified by qPCR with Library Quantification Kit- Illumina/ ABI Prism (KK4835, KAPA Biosystems). Cells yielding libraries >1nM by qPCR were normalised and pooled by vaccinee for sequencing.