RNA-Seq of Andrena sp.: library BPV_RNA
Lysate from a single AnBV-1 positive Andrena whole bee (negative for other common bee viruses) was RNAse treated (50 U Benzoase (Sigma) and 40 U RNase I (Thermo)). RNAse treatment augmented for (viral) encapsidated nucleic acids. > library constructed using TruSeq Stranded mRNAseq sample prep (without polyA selection or additional rRNA removal) (barcoded to distinguish from other samples in same lane) > 2 x 250 nt Illumina MiSeq sequencing.