GSM5800228: bb105ippo_1bis_S25_2ndDS; Homo sapiens; RNA-Seq
Samples were homogenized and total RNA was isolated by Trizol® reagent (Life Science Technologies, Italy) following the manufacturer's specifications. RNAs were quantified using a Nanodrop ND-100 Spectrophotometer (Nanodrop Technologies, Wilmington, USA) and a 2100 Bioanalyzer (Agilent RNA 6000 Nano Kit, Waldbronn, Germany); RNAs with a 260:280 ratio of ≥1.5 and an RNA integrity number of ≥2 were subjected to deep sequencing. Sequencing libraries were prepared by the SENSE Total RNA-Seq Library Prep Kit for Illumina with RiboCop (Lexogen) using 500-ng total RNA. Qualities of sequencing libraries were assessed with 2100 Bioanalyzer (Agilent DNA 1000 Kit, Waldbronn, Germany). RNA processing has been carried out using Illumina NextSeq 500 Sequencing.