GSM5901039: Panc02_sgNT_2; Mus musculus; RNA-Seq
RNA was isolated using TRIzol (Life Technologies). Starting from total RNA, poly-adenylated fragments were isolated, reverse transcribed and converted into indexed sequencing libraries using the KAPA stranded mRNA-seq kit (Sopachem, Eke, Belgium) RNA libraries were prepared for sequencing using standard Illumina protocols