GSM4792309: Untreated tumors Rep 3 - small; Mus musculus; RNA-Seq
RNA was extracted using the RNeasy Mini kit (QIAGEN) according to the manufactuer's instructions. Total RNA integrity was checked using an Agilent 4200 TapeStation (Agilent Technologies) and quantified using a Trinean DropSense96 spectrophotometer (Caliper Life Sciences). RNA-seq libraries were prepared from total RNA using the TruSeq RNA Sample Prep Kit v2 (Illumina) and a Sciclone NGSx Workstation (PerkinElmer). Library size distributions were validated using an Agilent 4200 TapeStation. Additional library quality control, blending of pooled indexed libraries, and cluster optimization were performed using a Qubit 2.0 Fluorometer (Thermo Fisher Scientific). RNA-seq libraries were pooled (6- to 8-plex) and clustered onto a flow cell lane.