GSM4971649: HCT116_TinT2_SP1_KO_2_rep2; Homo sapiens; ATAC-seq
omni-ATAC-seq ATAC seq library was prepared as described previously in PMID: 28846090. 50,000 cells were lysed for nuclei isolation. Transposition reaction was performed using Nextera DNA library preparation kit (NEB). Samples were amplified by eight PCR cycles for library preparation. Primer dimers and long DNA fragments were cleaned by AMPure XP beads purification step. DNA concentration was measured by Qubit fluorometric assay and library quality was determined by Bioanalyzer.