Whole-genome sequence of microbial electrolysis anaerobic digestion: suspension at 0.1V
Qualified DNA samples were randomly broken into fragments of about 350 bp in length with a Covaris ultrasonic breaker, and the entire library was prepared through the steps of end repair, A-tail addition, sequencing adapter addition, purification, and PCR amplification.When the library is constructed, use Qubit2.0 for preliminary quantification, dilute the library to 2ng/ul, and then use Agilent 2100 to detect the insert size of the library. After the insert size meets expectations, use the Q-PCR method to determine the effective concentration of the library. Accurate quantification (library effective concentration >3nM) to ensure library quality.After the library inspection is qualified, the different libraries are pooled according to the requirements of effective concentration and target data volume, and then Illumina PE150 sequencing is performed. Then library was then filtered into the clean data.