GSM5239283: ST15: striatal samples from C57BL/6J mouse 12; Mus musculus; OTHER
Animals were anesthetized using SomnaSol, perfused, and the brain was rapidly dissected. Brains were immediately placed in aCSF buffer in bubbling carbogen in a vibratome, and sliced coronally in 250 micron slices.The tissues were  mechanically disrupted by triturating using a patch pipet. Trituration was repeated 10 times to yield a homogenous suspension. Cell suspensions were diluted and passed through two 100 micron filters followed by two 40 micron filters. Cells were sedimented at 220 x g for 6 mins at 18°C, supernatant carefully removed, and the pellet was resuspended in 1mL aCSF-BSA. Centrifugation was repeated and pellet was resuspended in 1mL aCSF-BSA to yield an estimated 350-400 cells/microliter. Cells were then processed through a dead cell removal kit using standard methods. 10x Genomics Chromium Controller system was used for barcoding cells. Cells were concentrated by centrifuging at 300 x g for five minutes, and then diluted into PBS + BSA buffer at room temperature such that 33.8 microliters contained 500 cells. Each sample was loaded into an individual well of a Chromium Controller for generation of Gel-Bead in Emulsions (GEMS) and cell barcoding using the Chromium Single-cell 3' Reagent Kit (v2, 10x Genomics).