16S rRNA amplicons from microbial communities of the Gulfo Dulce water column
In 2018 and 2020, we used the primer pairs F515 (GTGYCAGCMGCCGCGGTAA, Parada et al. 2016) and R806 (GGACTACNVGGGTWTCTAAT, Apprill et al. 2015) modified from Caporaso et al. (2011) to amplify the variable fragment (V4) of the 16S rRNA gene, whereas in 2019, we applied the bacteria-specific PacBio primers 27F/1492R (AGRGTTYGATYMTGGCTCAG, RGYTACCTTGTTACGACTT) to synthesize near full length 16S rRNA amplicons.