Sequel genome sequencing of Eulachon: Adult Male Kidney
Kidney tissue from a single Eulachon from the Fraser River (caught near New Westminster) was stored in RNAlater until extraction. DNA was extracted using the Qiagen DNeasy Blood and Tissue Kit following standard protocol with RNase. DNA was shopped to CEGSQ sequencing centre in Montreal, QC for library preparation and sequencing. A single library was prepared using a 7-8kb+ size selection step, and sequenced across 6 total SMRT cells on a PacBio sequel instrument.