Illumina HiSeq 1500 sequencing; MDMs Solvent, AA, IL6, AA+IL6
MDMs Solvent, AA, IL6, AA+IL6
Cell culture dishes were harvested. monocytes differentiated into MDMs, cultured in R5 media (RPMI + 5 % human AB Serum + 1 % Sodium Pyruvate.  Media was changed 2 hours before treatment. Treated for 3 hours with the specified compound or solvent (EtOH) control. NucleoSpin RNA Kit (Macherey-Nagel, 740955.250); DNA-free™ DNA Removal Kit (ThermoFisher/Ambion, AM1906); according to manufacturer's protocol Libraries were created with the Lexogen  QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina.