GSM5800123: Control-2 [RNA]; Mus musculus; RNA-Seq
Total RNA was extracted from cell or animal tissue by Trizol reagent (Invitrogen) separately. The RNA quality was checked by Bioanalyzer 2200 (Aligent) and kept at -80℃.Part of RNA was utilized to construct rRNA depletion(NEBNext Ultra Directional RNA Library Prep Kit),The RNA with RIN >8.0 is right for cDNA library construction. RNA libraries were prepared for sequencing using standard Illumina protocols