DRD45NC
Genomic DNA was extracted from the donor parent DRD, recurrent parents SBA and 45NC, and their two NILs using CTAB from young leaves collected during the seedling period. The extracted DNA was evaluated using a fluorometer and diluted to a concentration of 30 ng/L for NGS. Raw reads were generated using paired-end sequencing (2  100150 bp) with the Illumina HiSeq 2000 and NextSeq platforms. As a pre-processing step, only reads with a minimum length of >25 bp and Phred score >20 were filtered using DynamicTrim and LengthSort of SolexaQA (version.1.13). The selected reads were mapped to the watermelon 97103 genome assembly version 2 using BWA (0.6.1-r104).