GSM6070195: H3K27ac, E8.25, WT, SHF, rep1; Mus musculus; ChIP-Seq
The E8.25 hearts were firstly fixed with 0.25% formaldehyde, then FHF/SHF were separated by forceps followed by methyl alcohol fixation. After PCR enrichment using 2X KAPA master mix, products were purified and selected by AMPure XP beads for 200-1000 bp.