WGS of Mycolicibacterium smegmatis MC2 155: knock-out mutant MSMEG_5570 (LigD protein), MSMEG_5580 (Ku protein)
The sequencing libraries were prepared, using the Vazyme TruePrep DNA Library Prep Kit V2 for Illumina preparation protocol (Vazyme Biotech Co.,Ltd, Nanjing, China). Whole-genome shotgun sequencing was performed on a NextSeq 500 platform, at a read length of 2 x 150 bp (300 cycles).