chip-seq_ABF2_60_N
15 days AREB1pro:GFP-AREB1 plants were treated with 5 mm KNO3 for 1 hour. Roots were collected and fixed in 1% formaldehyde for 15 min under vacuum at 25C. Isolated chromatin was sonicated with a Bioruptor sonicator (Diagenode, Belgium). A commercial antibody Anti-GFP antibody (catalog #A11122, Thermo Fisher Scientific, MA, USA) was used in ChIP experiments. Resulting ChIP DNA from each TFs was used to generate a sequencing library using the TruSeq DNA Sample Prep Kit (Illumina, San Diego, CA). Sequencing libraries were sequenced using an Illumina NextSeq 500 platform (Illumina, San Diego, CA).