GSM4905412: DOT1L KD HEL PRO-seq; Homo sapiens; OTHER
Nuclear run-on and RNA isolation, RNA fragementation and Biotin RNA enrichment. 3' RNA adaptor ligation and second biotin RNA enrichment, enzymatic modification of the RNA 5' ends, 5' RNA adaptor ligation and biotin RNA enrichment, Reverse transcription, PCR amplification and library size selection, and then sequencing.