Whole-genome sequencing of evolved P. putida KT2440
After genomic DNA is randomly broken into short DNA fragments by enzymes, blunt end repair is performed. Then dA tails are ligated at both ends of the DNA fragments, and sequencing adapters are ligated. The DNA fragments with adapters were purified by AMPure XP magnetic beads, and fragments in the range of 300-400 bp were selected for PCR amplification. The constructed library was purified, library checked, and sequenced on a Hiseq X10 PE150.