GSM5946230: PDL1-negative-1; Homo sapiens; RNA-Seq
Total RNA was extracted using ISOGEN (Nippon Gene) according to the manufacturer's instructions. PolyA-tailed mRNA was selected with beads using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Ipswich, MA). RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs, Ipswich, MA) according to the manufacturer's instructions.