GSM6069369: ChinmoST testis, D9-11, scRNAseq; Drosophila melanogaster; RNA-Seq
Approximately 5,000-10,000 cells were washed with 0.04% BSA DPBS for three times and were resuscitated and manually determined to a concentration of 700~1200 cells/ul (viability≥85%). Cells were captured in droplets at a targeted cell recovery of cells. After the reverse transcription step, emulsions were broken and Barcoded-cDNA was purified with Dynabeads, followed by PCR amplification. Amplified cDNA was then used for 10x Genomics chromium single cell 3ʹ gene expression library construction. For gene expression library construction, 50 ng of amplified cDNA was fragmented and end-repaired, double-size selected with SPRIselect beads.