RNAseq of Euphorbia peplus leaves
cDNA libraries were prepared from 100 ng of total RNA from latex and 150 ng from the other four tissues using the NEBNext Ultra II Directional Library prep kit from Illumina in conjunction with the NEBNext Poly(A) mRNA Magnetic Isolation Module, according to the manufacturers instructions. A 12-minute fragmentation time was used when eluting mRNA from polyA magnetic beads. Amplification of the final libraries involved 12 cycles of PCR and the NEBNext multiplex Oligos from Illumina.