GSM5964440: Liver SZ6; Mus musculus; RNA-Seq
Total RNA from liver tissues was isolated using TRIzol reagent according to the manu-facturer's instructions and RNA integrity was assessed using the RNA Nano 6000 As-say Kit of the Bioanalyzer2100 system (Agilent Technologies, CA, USA). mRNA was purified using poly-T oligo-attached magnetic beads. After fragmentation, libraries were constructed using NEBNext® Ultra™ RNA Library Prep Kit for Illumi-na® according to the manufacturer's instructions.