GSM5767677: S1C; Pyrus calleryana; RNA-Seq
Total RNA was extracted by the ethyl ammonium bromide (CTAB) method using seeds collected at 0, 20 and 50 days after cold stratification with three biological replicates. RNA-Seq library was generated with NEB Next® UltraTM RNA Library Prep Kit for Illumina (NEB, USA) and library quality was assessed on the Agilent Bioanalyzer 2100 system.