GSM6285441: Non_Pregnant_NSG_Lox_Donor_1; Homo sapiens; RNA-Seq
Gel Beads in Emulsion (GEMs) were generated using the Chromium 3' Single Cell Library Kit (v2, 10X Genomics, CA) according to the manufacturer's instructions. Briefly, 10,000 nuclei were combined with Single Cell Master Mix and encapsulated into the barcoded Gel Beads through the Chromium™ Controller. After GEM-RT incubation, cDNA samples were recovered, purified and amplified through a cDNA Amplification Reaction. Quality controls on amplified cDNA samples were carried out through using a High Sensitivity DNA Kit (Agilent, CA) on a 2100 BioAnalizer (Agilent, CA) platform. Libraries were then constructed following Fragmentation and Adaptor Ligation and Sample Index incorporation. Finally, purified libraries were run on 2100 BioAnalizer (Agilent, CA) using a High Sensitivity DNA Kit (Agilent, CA) to evaluate the quality of the ~400bp fragments.