Illumina HiSeq 1500 sequencing; Human monocyte derived macrophoges exposed to arachidonic acid and interferon beta
Human monocyte derived macrophoges exposed to arachidonic acid and interferon beta
Cell culture dishes were harvested. Monocytes obtained from 3 individual donors were cultured for 6 days in RPMI 5% FCS 1% sodium pyruvate, differentiating them into monocyte-derived-macrophages. Treated for 3h with solvent (LSM) 50 µM (Arachidonic Acid Biomol Cay90010-100), 20 ng/ml IFNb (Interferon beta1a, human recombinant (rHuIFN-beta1a-CHO)Biomol Art# 86421.20) or both. NucleoSpin RNA Kit (Macherey-Nagel, 740955.250); DNA-free™ DNA Removal Kit (ThermoFisher/Ambion, AM1906); according to manufacturer's protocol Libraries were created using the Illumina Truseq mRNA kit v2 used according to the manufacturer's instructions.