GSM4792251: Regenerative cardiac cells - mRNA_0dpa_rep2; Danio rerio; RNA-Seq
For each sample, fixed tissues were lysed to prepare nuclear extracts. After chromatin shearing by sonication, lysates were incubated overnight at 4°C with protein A Dynabeads (Invitrogen) coupled with 3-5 μg of antibody specific for H3K9Ac or H3K9Me3. ChIP-seq libraries were generated by ThruPLEX-FD Prep Kit (Product No.R40012, Rubicon, Ann Arbor, USA), and sequenced by Illumina Hiseq 2500 using the paired-end module and with 150 bp reads on each end. the mRNA-seq library preparation from 1 μg of total RNA was performed with TruSeq RNA Sample Prep Kit (Illumina) according to the manufacturer's instructions, and 2 x 150 paired-end sequencing performed on Illumina Hiseq 2500.