SARS-CoV-2/human/Marburg/PUM-D2417/2021
Patient isolate D2417 was cultivated and first passaged on VeroE6 TMPRSS2 cells, then on Clau3 cells and again on VeroE76 TMPRSS2 cells. In total 3 Passages. Virus was concentrated by ultracentrifugation, RNA was isolated using RNeasy (Qiagen) Kit according to manufactures protocol. Library was prepared using NEB Ultra II RNA directional and UDI according to manufactures protocol.