Degradome sequencing of jojoba: leaf
A degradome library was constructed from pooled RNA samples from both leaves and roots according to a previously described method63 with minor modification. Briefly, approximately 150 ng of poly (A) RNA was used as input RNA and annealed with biotinylated random primers. After RNA fragments were streptavidin captured using biotinylated random primers, the RNAs with monophosphate at 5 end were ligated to a 5 adaptor. Next, reverse transcription and PCR were performed. The resulting library was single-end sequenced using an Illumina Hiseq2500 platform at the LC-BIO (Hangzhou, China) following the vendors recommended protocol