GSM5708184: CVID patient with villous atrophy 17; Homo sapiens; RNA-Seq
RNA was extracted using RNeasy Plus Mini Kit (Qiagen) according to the manufacturer's instructions Libraries were prepared from high quality total RNA samples (~200 ng/sample) using the Illumina TruSeq RNA Sample Preparation v2 Kit (Illumina) implemented on the liquid handling robot Beckman FXP2. Obtained libraries that passed the QC step were pooled in equimolar amounts; 1.8 pM solution of this pool was loaded on the Illumina sequencer NextSeq 500 and sequenced uni-directionally, generating ~500 million reads, each 85 bases long.