RNA-seq of Medicago truncatula shoots from a plant colonzied by Glomus aggregaturm strain 165, harvested at seven weeks post-inoculation
Extracted RNAs were treated with TURBO DNase (Thermo Fisher Scientific) and quantified using a NanoDrop ND-2000 spectrophotometer (Thermo Fisher Scientific). RNA quality was assessed using 2100 BioAnalyzer technology (Agilent Technologies). Approximately 100 ng of total RNA was used to construct poly(A) selection libraries using the Illumina TruSeq RNA Sample Preparation kit.