GSM4970530: 5638; Mus musculus; RNA-Seq
Genomic DNA, total RNA were extracted from approximately 10 mg of proximal colon tissue using AllPrep DNA/RNA mini kit (QIAGEN, Hilden, Germany). RNA-seq libraries were prepared by Clontech Smarter Stranded Total RNA-Seq Kit (Takara Bio Inc, Kusatsu, Japan) according to manufacturer's instruction.