MicroRNAs-seq of tea leaves infected by Epicoccum nigrum for 48 h
Tea leaf spot caused by E. nigrum (ACCC39733) was firstly found in Sinan County, Guizhou Province, China.Mycelial plugs (6 mm diameter) from the edge of colony of the strain ACCC39733 infected in tea leaves at 25C for 48 h before tea leaves harvested, ground with liquid nitrogen. About 100 mg powder was used for RNA extraction.The libraries of miRNA sequencing were prepared using TruSeq Small RNA Sample Prep Kits (Illumina, San Diego, USA) following the manufacturer's procedure. The libraries were sequenced on an Illumina Hiseq 2500 (LC Sceiences, USA) at the following the vendors recommended protocol,then following the manufacturer's procedure.