Whole-genome sequencing of population isolated at day 84 from mouse WD3
Library construction and sequencing were performed at the genomics facility at Instituto Gulbenkian de Ciencia (Oeiras, Portugal). Nextera XT kit from Illumina was used for library construction. Briefly, each population was defrosted onto BHIS-Ery-Gen-agar and incubated anaerobically at 37C for 48h. DNA was then extracted from these population of single colonies for whole genome sequencing on a NextSeq Benchtop Sequencer.