RNA-Seq of M. truncatula  and S. meliloti: NaCl - 2
RNAs were prepared as described in Sallet et al., 2013 (Sallet, E., Roux, B., Sauviac, L. et al. (2013) Next-Generation annotation of prokaryotic genomes with EuGene-P: application to Sinorhizobium meliloti. 2011. DNA Res. 20, 339-354.). Briefly, total RNA were extracted using MirVana miRNA Isolation Kit (Ambion). RNA were treated with Turbo DNAse (Ambion) prior to ribosomal RNAs depletion by an oligocapture strategy derived from the Plant Ribominus kit (Invitrogen), in which the oligonucleotide sets were specifically designed to target M. truncatula and S. meliloti rRNAs, as well as the highly abundant S. meliloti tRNA-Ala. Samples were then treated using Turbo DNAse (Ambion) and purified on Zymo Research RNA Clean and ConcentratorTM-5 columns (Proteigene).