GSM6214480: in vivo mouse xenograft HCC2302 A947 rep1; Homo sapiens; RNA-Seq
For in vitro gene expression studies, cells were treated with DMSO or A947(100nM) for both 24h and 96h prior to isolation of total RNA using the MagMax mirVana total RNA isolation kit (ThermoFisher Scientific, A27828). In addition, HCC2302-shNTC and HCC2302-shSMARCA2 cells were treated for 168h prior to isolation of RNA. For in vivo gene expression studies, total RNA was isolated from xenograft tissues as above. RNA concentrations were measured by NanoDrop8000 (ThermoFisher). Integrity of RNA was assessed by Bioanalyzer 2100 prior to library generation using 500ng RNA. Libraries were prepared using the TruSeq Stranaded Total RNA Library Prep Kit (Illumina), multiplexed and sequenced on Illumina HiSeq2500 (Illumina) to generate ~30M single end, 50 base pair reads.