GSM5900842: DbmDMSO DbmD2; Mus musculus; RNA-Seq
Mice glomeruli were isolated as previously reported. Total RNA was extracted from glomeruli using Trizol method. Purified RNA underwent DNA digestion using RNase-free DNase set (79254, Qiagen). RNA sequencing was performed at Beijing Genomics Institute. Purifed RNA underwent DNA digestion using RNase-free DNase  set (79254, Qiagen, Germantown, MD). Only samples with an RNA  integrity value of > 7.5 were used for mRNA sequencing at the CLC Genomics and  Epigenomics Core Facility at Weil Cornell Medical College for library construction and sequencing.