Illumina-seq of Trogolaphysa sp.: adult whole body
After whole genome amplification samples were qualified, randomly interrupted using a Covaris ultrasonic disruptor, and then the entire library preparation is completed through the steps of end repair, A-tailing, sequencing adapters, purification, and PCR amplification. After the library is constructed, Qubit 2.0 was used for preliminary quantification, library was diluted, and then we use Agilent 2100 to detect the inserts in the library. After the insert size meets expectations, use the Q-PCR method to accurately quantify the effective concentration of the library.  After the library is qualified, different libraries are pooled to flowcell according to the effective concentration and target data volume requirements, the cBOT is clustered and sequenced using the Illumina high-throughput sequencing platform.