GSM5964517: DI318 CSCs + C16 rep1; Homo sapiens; OTHER
Cells were harvested, counted and CUT&Tag-IT Assay and library preparation was performed on 500,000 cells per replicate, according to the Manufacturer's Manual (Active Motif). Rabbit anti-human Tri-Methyl-Histone H3 (Lys4) (C42D8) (Cell Signaling Technologies 9751) antibody was used for the CUT&Tag procedure. Sample indices for multiplexing (unique combination of one i7 Indexed Primer and one i5 Indexed Primer) were attached through 14 rounds of PCR.