GSM6254147: M1 + L1/2_biol, rep3; Homo sapiens; OTHER
Cells were harvested and genomic DNA was extracted using GeneJet Genomic DNA Purification Kit (Thermo Fisher) following manufacturers protocol. Concentration of isolated gDNA was measured with Nanodrop2000 and stored until further use at -80 °C Targeted amplicon deep sequencing was performed. Locus of interest was amplified through PCR with barcoded Primers for multiplexing. In total 100 ng gDNA were used together with 0.5 µM each forward and reverse primer and 0.2 µM dNTPs and 0.2 µl phusion polymerase (Thermo Fisher). In total three independent PCR reactions were merged. All barcoded amplicons were pooled in an equal molar ratio.