GSM5800128: NPWT-1 [miRNA]; Mus musculus; miRNA-Seq
Total RNA was extracted from cell or animal tissue by Trizol reagent (Invitrogen) separately. The RNA quality was checked by Bioanalyzer 2200 (Aligent) and kept at -80℃.The RNA with RIN >8.0 is right for miRNA purification. miRNA was purified by miRNeasy Mini Kit (Qiagen) and the purification result was validated by Gel electrophoresis. RNA library using standard Ion proton RNA-Seq Kit v2.0 protocol