GSM6069483: Col-0 (WT), biol replicate #1; Arabidopsis thaliana; RNA-Seq
Total RNA was extracted using the RNeasy Plant Mini kit (Qiagen) and quantified using a Flurometer (Qubit 2.0, Thermo Fisher). 500 ng of total RNA was used to isolate poly(A)-mRNA using the NEBNext poly(A) mRNA Isolation Module (NEB) and the purified mRNA was used to construct sequencing libraries using the Ultra II Directional RNA library Prep Kit for Illumina (NEB)