GSM5627828: M2; Bacillus subtilis; RNA-Seq
Total RNA from Bacillus strains was isolated with TRIzol reagent (Invitrogen, USA). The ribosomal RNA was removed using the Ribo-Zero rRNA removal kit (Illumina, CA, USA, MRZB12424). The RNA was then fragmented to 200-300 bp, followed by random primers and reverse transcriptase to synthesize the first strand of cDNA, and then the second strand of cDNA. The library size was then measured using an Agilent 2100 Bioanalyzer and the cDNA was sequenced on the Illumina HiSeq 3000.