GSM6253445: 1-BLBX; Homo sapiens; RNA-Seq
Total RNA for Library Preparation and Sequencing analysis was purified from tumor tissue using the RNAeasy Plus Kit (QIAGEN) that included a genomic DNA elimination step. Coding RNA transcripts were enriched using human exome panel hybridization (Twist Biosciences).  Size, concentration, and integrity was verified using Agilent 2100 Bioanalyzer (Agilent Technologies). Libraries were generated using Illumina's TruSeq RNA sample Prep Kit v3, following the manufacurer's protocol. Sequencing of 8–10 pM of each library was done on the NovaSeq sequencer as 100bp paired-end read runs.