Methylome capture of HG005 using Accel-NGS Methyl-Seq
100 ng of genomic DNA was spiked in with 1% unmethylated Lambda gDNA, and fragmented to 350 bp (Covaris). Bisulfite conversion was performed using EZ DNA Methylation-Gold kit (Zymo Research). Libraries were prepared according to manufacturers instructions (Swift), using dual-indexing primers. A total of 6 rounds of amplification were performed using the Enzyme R3 provided with the kit.