GSM4971452: Adip.RNAseq.A1.DMSO.2; Homo sapiens; RNA-Seq
Total RNA was extracted by using Qiagen RNeasey Mini kit. DNA was isolated using phenol/chloroform extraction and NaCl/EtOH precipitated with 20 μg glycogen carrier. Transposed DNA was purified using a QIAGEN MinElute Reaction Cleanup kit. Transposed fragments were subjected to library preparation following manufacturer protocol The H3K4me3 in-situ ChIA-PET was performed according to the previously described protocol (Li et al., 2017) RNA libraries were prepared for sequencing by Novogene (CA). ChIP-seq library was prepared for sequencing according to the amplification protocol from Illumina. ATAC-seq libraries were sequenced using paired-end, dual-index sequencing on a NextSeq 500 instrument The H3K4me3 in-situ ChIA-PET was performed according to the previously described protocol (Li et al., 2017)