gRNA sequencing of library-transduced Raji cells: unsorted control 1
Raji cells have been transduced with the Brunello Human genome-wide CRISPR Cas9 knock-out library. After antibiotic selection, cells were split into those subjected to sorting and unsorted control. The gDNA of unsorted cells was isolated, the contained gRNAs PCR-amplified and analyzed via Illumina NGS.