V3-V4 16S from Sycettusa hastifera: Praia do Forno_replica1
The total DNA was extracted following a modified phenol-chloroform protocol. DNA samples were then sent for Illumina sequencing at Macrogen Inc., South Korea. Sample preparation and library construction were conducted following manufacturers instructions within the companys workflow for metabarcoding analyses. The hypervariable region V3-V4 of the 16S rRNA gene was then sequenced on the Illumina MiSeq platform (2x300 bp) using the primers Bakt_341F (CCTACGGGNGGCWGCAG) and Bakt_805R (GACTACHVGGGTATCTAATCC)