halophilic microorganism
The genomic DNA of the sample was extracted by SDS or STE, then the purity and integrity of DNA were detected by agarose gel electrophoresis, and quantified by Qubit. DNA samples qualified by electrophoresis were randomly broken into fragments with a length of about 350bp by Covaris ultrasonic breaker. The finished DNA fragment was prepared by using Nebnex Ultra DNA Library Prep Kit for Illumina (Neb, USA) kit, and the whole library was prepared through the steps of terminal repair, adding A tail, adding sequencing adapter, purification and PCR amplification.