GSM4906708: CTL mice 2 [49]; Mus musculus; RNA-Seq
Total RNA was extracted from control and MCOPPB-treated mice liver using TRIzol Reagent (ThermoFisher Scientific, Waltham, USA). Indexed libraries were prepared from 250 ng purified RNA using a NEB Next Ultra II Directional RNA Library Prep Kit (Illumina, UK), according to the manufacturer's instructions. The libraries were pooled in equimolar amounts, subjected to cluster generation and sequenced on an Illumina MiSeq System (Illumina) in a 1x75 format. Quality control was performed by FastQC and MultiQC.