GSM4792206: runx1-/- kidney2; Danio rerio; RNA-Seq
RNA-seq on kidney: Wild type and runx1del25/del25 kidneys were dissected from ~2.5 months old fish (n= 3 for each genotype, pool of 3 kidneys/replicate) and immediately collected in Trizol (#15596018, Invitrogen). Samples were then put in a heatblock at 52oC for 10 min and then overnight in -80oC. RNA was extracted following the manufacturer protocol using Direct-zolTM RNA MiniPrep Kit (Zymo Research #R2051). Sequencing libraries were prepared using paired-end library preparation (TruSeq RNA Library Prep Kit v2).