RNA-seq of Pseudomonas putida stationary phase
Total RNA was purified by hot phenol, DNAseI digestion, MiniElute cleanup, and depleted from rRNAs using the Ribo-Zero rRNA removal kit (EpiCentre). cDNA libraries were generated using a strand-specific ScriptSeq Complete Kit Bacteria protocol (Epicentre), indexed and sequenced with 101-nt single-end reads.