GSM5014446: shh1-1_Lv_BS-seq; Arabidopsis thaliana; Bisulfite-Seq
~0.1 g of tissue from either 15 day old rosettes or leaves, and >5,000 unfertilized ovules were collected for genomic DNA isolation using the DNeasy Plant Mini Kit (Qiagen, Cat# 69104). MethylC-seq libraries were generated using 2.0 μg of DNA from rosettes and leaves, and 0.5-1.0 μg of DNA from ovules as described in Li et al. (2017). The libraries were then pooled and sequenced (single end 50 bp, SE50) on a HiSeq 2500 machine (Illumina).