GSM5900601: NMP-L cells DAY 3 RNA-seq biological replicate 2; Homo sapiens; RNA-Seq
Culture medium was removed and cells were lysed with RLT buffer (Qiagen Rneasy Kit). Samples were collected and stored at -80 until ready to batch process. Samles were DNAse1 digested and RNA extracted as per manufacturer instructions. The DNA library for sequencing was prepared from Qiagen column purified DNA. Libraries were prepared using the Kapa mRNA HyperPrep kit. DNA quality was confirmed by Agilent TapeStation prior to library preparation using NEB Ultra II DNA. Libraries were sequenced as single-end, 76bp reads on the Illumina High-Seq 4000 platform (Francis Crick Institute).