Methylome capture of HG007 using EMSeq with 100ng of input DNA
100, 50 and 10 ng of genomic DNA spiked in with 2 ng unmethylated lambda and 0.1 ng CpG methylated pUC19 was fragmented to 500 bp (Covaris S2, 200 cycles per burst, 10% duty-cycle, intensity of 5 and treatment time of 50 seconds). EM-seq libraries were prepared using the NEBNext Enzymatic Methyl-seq (E7120, NEB) kit following manufacturers instructions. Final libraries were amplified with the included NEBNext Q5U polymerase using 4 cycles for 100 ng, 5 cycles for 50 ng and 7 cycles for 10 ng inputs.