GSM5239389: HPC-1; Homo sapiens; RNA-Seq
Total RNA was extracted using Trizol Reagent from the cells.Sequencing libraries were generated using NEBNext ® Ultra™ RNA Library Prep Kit for Illumina ® (#E7530L, NEB, USA) according to manufacturer's instructions. Following analysis of the library, RNA sequencing was completed using the Illumina Hi Seq X Ten sequencing platform, and paired-end sequence reads were obtained. RNA libraries were prepared for sequencing using standard Illumina protocols