GSM5900068: XH001 + TM7x passage 4 time 15 rep 3; Schaalia odontolytica; Candidatus Nanosynbacter lyticus; RNA-Seq
Cell pellets were resuspended in cold PBS buffer and beaten with glass beads 3 times for 30 seconds with 30 second breaks at 6 m/s speed. Total RNA was isolated using the High Pure RNA isolation kit. Isolated RNA was cleaned and concentrated with Zymo Research RNA clean & concentrator kit following kit protocol. DNA was removed using the Ambion TURBO DNA-free kit. rRNA was depleted with Ribo-Zero. RNA sequencing library preparation used NEBNext Ultra RNA Library Prep Kit