Genomic sequencing of rhabdomyosarcoma/Pathogenic Germline Variants in Cancer Susceptibility Genes in Children and Young Adults With Rhabdomyosarcoma: Sample RMS219_blood
"Genomic DNA was extracted from patient's blood using Paxgene blood DNA kits. One hundred nanograms of DNA was sheared to approximately 300 base pairs (bp) by sonication (Covaris, Woburn, MA). DNA library construction and enrichment was performed using SeqCap EZ Exome v3.0 Kits according to the manufacturer's instructions (Roche, Wilmington, MA) . Paired-end sequencing (2X125 bp) was performed on an Illumina HiSeq2000 instrument."