RNA-Seq of Jatropha curcas  (treated sample)
Total RNA of Jatropha curcas were isolated from treated Jatropha curcas leave using Rneasy Plant Mini kit (Qiagen). RNA is converted to cDNA by reverse transcription. Fragments are converted into the library by ligation to sequencing adapters containing specific sequences by Illumina. Next step involve clonal amplification of the library through cluster generation and finally generating sequence.