Pond, water entrance, Algae
18S rRNA genes were amplified by PCR using MyTaq DNA Polymerase (Meridian Biosciences) using universal eukaryotic primers obtained from [20] DeLong (1992): EukF Forward Primer (5-AAC CTG GTT GAT CCT GCC AGT-3) and EukR Reverse Primer (5-TGA TCC TCC TGC AGG TTC ACC TAC-3). PCR conditions for the reaction were: 95 C for 3 min, 35 cycles of 95 C for 30 s, 60 C for 15 s, 72 C for 45 s and a final extension step at 72 C for 5 min. Successful PCR amplification products of ca. 1 kb (check this is still the case after data) and 1.5 kb bands were extracted using the QIAquick Gel Extraction Kit (QIAGEN, Hilden, Germany) and outsourced to Macrogen Ltd. (The Netherlands) for Sanger-sequencing.