GSM5944706: KYSE-30 METTL3KO_rescuewithmutMETTL3_TET1_CUTtag_rep3; Homo sapiens; OTHER
Total RNA was extracted using TRIzol reagent (Invitrogen). Genomic DNA was isolated with QIAmp DNA Mini kits (Qiagen). Sequencing libraries for m6A-IP and input were prepared following the manufacturer's instructions and sequencing was conducted using Illumina HiSeqX Ten PE150 platform. The WGBS library was sequenced on an Illumina HiSeq2500 sequencers and generated paired-end reads (2× 125 bp). CUT&Tag sequencing was performed in the Illumina Novaseq 6000 using 150bp paired-end following the manufacturer's instructions.