RNA-Seq of Elysia viridis: adult, starved 1 week under complete darkness
The specimen was fixed in liquid nitrogen and subsequently freeze dried at -80 C for 2 days. Total RNA was extracted using the myBudget RNA Mini Kit (Bio-Budget Technologies, Germany) following the manufacturers instructions. The RNA concentration was quantified with Qubit fluorometric quantification (ThermoFisher, USA) and NanoDrop One/OneC (ThermoFisher, USA). Poly(A) mRNA enrichment, library preparation using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, USA), and 150bp paired end sequencing using the Ilumina NovaSeq system was done by Novogene (UK).