GSM6285578: BM-ddW 1; Mus musculus; RNA-Seq
Cells were lysed with cell lysis buffer (Roche). The first strand of cDNA was synthesized with the PrimeScript RT reagent kit (TAKARA Bio Inc.) and NSR (not-so random) primers. The second strand was synthesized with Klenow Fragments (3′-5′exo-; New England Biolabs Inc.) and complement chains of NSR primers. The double-stranded cDNA was purified with AMPure XP beads (Beckman Coulter, Inc. CA). The library was preparation with the Nextera XT DNA sample Prep kit. The quantity and quality of the isolated cDNA library was determined with Multina (Shimadzu Corporation, Japan) and Bioanalyzer 2100 (Agilent, Waldbronn, Germany).