GSM5946285: RH1_WT-Nut3; Mus musculus; RNA-Seq
Dissection immediately after eutanasia, the tissue was frozen in -80 degree freezer until use essenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.  single read on Illumina sequencer HWI-ST1276, flow cell C1162ACXX; Illumina index sequence: CGATGT