RNA-Seq data from reproductive tissues of mated female flies at 2-4 hours post mating - Biological Replicate 2
Reproductive tract tissues (ovary, oviduct, uterus, spermatheca) were dissected from cold anesthetized females. Tissues were homogenized in trizol reagent and RNA isolated using the Direct-zol kit from Zymo. RNA quantity and quality were verified on an Agilent bioanalyzer. PolyA RNA was purified from total RNA and used for library production using the Illumina Stranded mRNA Prep kit.