GSM5133554: A4_BF_2: adult NCSs (ANS4) treated with BMP4+FGF2 replica 2; Mus musculus; RNA-Seq
RNA extraction was performed using the Qiagen RNeasy Plus minispin column kit, eluting in 50 µL of RNase-free water, and using an additional DNase step. RNA concentration was determined using the Qubit RNA High-Sensitivity kit (Life Technologies). Sample concentration and quality was assessed using the Aligent Tapestation and Nanodrop, only samples with a RIN value of 9.6 or above were used. For RNAseq we used RNA-Seq Quantification Library (Low input Library) BGISEQ-500RS.