Illumina NovaSeq 6000 paired end sequencing; single nucleus RNAseq of mouse ingWAT with Rspo2 or GFP AAV injection
single nucleus RNAseq of mouse ingWAT with Rspo2 or GFP AAV injection
ingWAT was harvested. ingWAT was harvested 10 weeks of high fat diet at 5 week of age AAV was injected via tail vein at 9 week of age standard 10x protocol was applied standard 10x protocol was applied