GSM6069557: BRF22485-WT2-19W; Mus musculus; ncRNA-Seq
Lysates of 250 mg brain tissue were subjected to pull down with a GW182 peptide bound to anti-FLAG M2 Magnetic beads RNA at a size of 19-35 nt was gel extracted and used to generate library The quality and quantity of the RNA samples was checked using an Agilent bio-analyzer. Small RNA-SEQ libraries were generated using Illumina small RNA SEQ kits using the Illumina provided protocol containing library fragments 140-160 bp in size. RNA-Seq; sequenced on an Illumina HiSEQ4000 using Illumina provided reagents and protocols