miRNA-Seq of Aborting flower buds: Lotus
Lotus "Boli Furen" used in this study was planted in a large pool of 22 meters under natural light conditions in April (32N119E, Nanjing city, China), and was sampled in June when there were enough leaves to obtain flower buds. Flower buds with similar morphological size and at the same differentiation stage were collected based on the method of sampling and identification of flower bud differentiation period in vivo invented by our laboratory (Hu Xin, 2018), and normal flower buds and aborted flower buds were judged by the naked eye. Three biological replicate were collected and each independent repeat contains multiple flower buds, about 0.5 grams. The samples were quickly frozen in liquid nitrogen and stored in the refrigerator at -80 C for subsequent small RNA sequencing.