Hi-C sequencing of Dalbergia cochinchinensis (Oxon_Dacoc_HiC_1)
We harvested 0.5 g of fresh leaf from the reference individual and immediately cross-linked the finely chopped tissue in 1% formaldehyde for 20 minutes. The cross-linking was then quenched with glycine (125 mM). The cross-linked samples were then ground in liquid nitrogen with a mortar and pestle for library preparation. The Hi-C library was prepared with the restriction enzyme DpnII, proximity-ligated, and reverse-crosslinked using the Phase Genomics Proximo Plant Kit v2.0 (Phase Genomics, Seattle, USA). The library was sequenced on a HiSeq4000 for ~300 M 150-bp paired-end sequencing.