GSM5899994: E_53h3; Zea mays; miRNA-Seq
The embryos of five individual seeds were dissected with a sterile knife in each sample and then the samples were immediately frozen in liquid nitrogen and stored at −80 °C.Total RNA was extracted using RNAprep Pure Plant Kit (Tiangen) following the procedure of the manufacturer. The concentration was measured by Nanodrop 2000 and integrity of total RNA was tested on 1.0% agarose gel. Small RNA library construction was conducted with TruSeq Small RNA Sample Prep Kits (Illumina, San Diego, USA). Small RNA libraries were sequenced with Illumina Hiseq2000/2500 and the sequencing read length is single-ended 1×50bp.