GSM5707551: PTZ-1; Rattus norvegicus; RNA-Seq
Total RNA was extracted from hippocampal tissue sample (about 100 mg)  using TRIzol kit (Invitrogen, USA)  according to the manufacturer's instructions. RNA purity was checked and quantification were evaluated using the NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, USA). Samples with an absorbance (260/280) ratio between 1.8 and 2.2 were considered acceptable. Agilent 2100 Bioanalyzer (Agilent Technologies,  USA) was used to assess the RNA integrity. The sequencing library was generated by using MGIEasy Universal Library Convert Kit (MGI, China), VAHTS® Universal V6 RNA-seq Library Prep Kit for Illumina (Vazyme, China), and VAHTS® mRNA Capture Beads (Vazyme, China) according to the manufacturer's protocols.