metagenome sequencing of sample EC2
Ten grams of the homogenized tissue sample was extracted using the MO-BIO Powermax Soil DNA Isolation Kit according to manufacturing protocol with the addition of 400 g/ml Proteinase K. All DNA extracts were purified using MO-BIO PowerClean DNA Clean-Up Kits and quantified using Qubit dsDNA HS Kit, run on 1% agarose gel in sodium borate buffer to check quality of DNA.