Pond, water entrance
The DNA extraction from 0.25 g of samples of sediments and streamers was done using a QIAGEN DNeasy PowerLyzer PowerSoil Kit (QIAGEN, Hilden, Germany) according to the manufacturers protocol, quantified using BR dsDNA Assay kit and Qubit fluorometer (Invitrogen, Carlsbad, CA, UK) and PCR-amplified with the 16S rRNA gene V4 region-specific primers following the same procedures as specified earlier by [13] Distaso et al. (2020). The purified amplicons were sequenced using the Illumina MiSeq platform (Illumina, San Diego, CA, USA) using 500-cycle v2 chemistry (2250 bp paired-end reads) at the Centre for Environmental Biotechnology, Bangor, UK.