NextSeq 500 sequencing; ChIP-Seq data from Nup133 ChIP performed on primary male and female mouse derived oligodendrocyte precursor cells
ChIP-Seq data from Nup133 ChIP performed on primary male and female mouse derived oligodendrocyte precursor cells
Mouse OPCs were isolated from enzymatically dissociated post natal day 2-4 old CD-1 (wild type strain) mice brains. For each isolation, tissue from the mid brain region comprising the sub ventricular zone from 3 male and 3 female (from the same litter) brains were pooled separately. Chromatin immunoprecipitation was performed using the MAGnifyTM Chromatin Immunoprecipitation System kit (Thermo Fischer). The manufacturersâ€™ protocol provided with the kit was followed to perform the experiments and extract nucleic acid. Nucleic acid libraries were constructed using the protocol mentioned in Illumina TruSeq nano DNA Library prep kits.