WGS from human saliva
Saliva samples were collected in the Oragene DISCOVER ORG-500 tube and DNA was extracted using the Oragene PrepIT L2P extraction kit. Saliva samples were collected at the time of tumor collection. DNA sequencing libraries were prepared with the KAPA Hyper Prep kit, and samples were sequenced to a target depth of 60x coverage (for tumor) or 30x coverage (for saliva) on an Illumina NovaSeq