NextSeq 500 sequencing; RNA-seq of prostate cancer Patient‐Derived Xenografts derived from primary and metastatic tumors at different stages of progression
RNA-seq of prostate cancer Patient‐Derived Xenografts derived from primary and metastatic tumors at different stages of progression
Tumor tissue was extracted from scarified mice, fragmented and immediately frozen. RNA was extracted using the Rnasy Mini Kit (Qiagen) and sample quality was assessed using Agilent Bioanalyzer. RNA libraries were prepared for sequencing using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®