Genomic sequencing of rhabdomyosarcoma/Genomic Classification and Clinical Outcome in Rhabdomyosarcoma: A report from an International Consortium. : Sample RMS2334
DNA was extracted using high temperature and Proteinase K followed by extraction with the QIAamp MinElute spin column DNA Extraction Kit (Qiagen) or the QiaAmp DNA FFPE Tissue kit (Qiagen) (UK). Twenty to 100 nanograms of DNA was used for sequencing library preparation with the KAPA Library Preparation Kit for Illumina platforms (Kapa Biosystems). A custom capture kit for the coding regions of 39 genes previously described as mutated or with copy number aberrations in RMS and previously included on pediatric panels was designed and generated using the NimbleDesign tool (Roche). Paired-end sequencing (2X75 bp) was performed on an Illumina NextSeq500 instrument.