GSM6213996: NSC,Control,scRNAseq; Mus musculus; RNA-Seq
Plated neural progenitors were dissociated using 0.05% Trypsin-EDTA 4hrs after electroporation (BEP and LEP). The cells were then washed are resuspended in PBS for viability analysis (Trypan Blue) prior to library preparation and sequencing. Single cell gel bead generation and barcoding was performed with 10,000 cells from each experimental condition using the NextGEM single cell 3' v3 expression chip on the Chromium platform (10x Genomics) using maufacturer's protocols.