GSM5482573: miRNA-seq_Ethanol-exposed rep 1; Homo sapiens; miRNA-Seq
hESC-derived neural cells (exposed or unexposed to ethanol) were collected, flash frozen on dry ice, and installed at -80 degree freezer. Total RNAs were extracted using the QIAGEN miReasy Mini Kit. Small RNA-seq libraries were generated using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (Set 1) (NEB, Ipswich, MA, USA) with 250 ng of total RNAs. RNA libraries were prepared for sequencing using the protocol for use with NEBNext Small RNA Library Prep Set for Illumina