GSM6213918: MEFs, Δ-C60, Pol II ChIP-seq, biol rep 2; Mus musculus; ChIP-Seq
Cells were treated with IAA at 0.5mM for 6 hours, if indicated, and then fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was fragmented with micrococcal nuclease and immunoprecipitated with Pol II or flag antibodies. ChIP-seq libraries were prepared using the KAPA Hyperprep Library Preparation Kit complemented with Roche SeqCap Adapter Kit A.  Post amplification libraries were size selected at 0.7-0.9X using Kapa cleanup beads. Libraries were validated using the Agilent High Sensitivity DNA Kit.