dual RNA-seq of host-pathogen interaction of Salmonella Enteritidis with granulosa cells of duck
enriched mRNA was fragmented into short fragments using fragmentation buffer and reverse transcripted into cDNA with random primers. Second-strand cDNA were synthesized by DNA polymerase I, RNase H, dNTP and buffer. Then purified with QiaQuick PCR extraction kit, end-repaired, poly (A) added, and ligated to Illumina sequencing adapters.