GSM5628781: Control E4.5 Embryo replicate 8; Mus musculus; RNA-Seq
RNA extraction and cDNA synthesis was performed using SMARTseq-V4 kit. cDNA was then purified with AMPure XP beads RNA-seq libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB E7645), diluting NEB adaptors 1:5 and performing 8 PCR cycles.