16S sequencing of Bombyx mori feces metagenome
Amplicon  quality  was  visualized  using  gel  electrophoresis,  purified  with  AMPure  XP  beads (Agencourt),  and  amplified  for  another  round  of  PCR.  After  purified  with  the  AMPure  XP  beads again, the final amplicon was quantified using Qubit dsDNA assay kit. Equal amounts of purified amplicon were pooled for subsequent sequencing.