GSM5129924: NS-SV-AC RES depc transfection replicate 1; Homo sapiens; RNA-Seq
Total RNA was extracted from cell lysate using TRIzol after centrifugation at 10,000 g for 30 sec. After precipitation of the extracted nucleic acids, DNAse I was treated to remove DNA. 2x101 paired-end RNA-seq library was constructed using TruSeq Stranded mRNA Prep Kit and sequenced using the Illumina Novaseq6000 system.