GSM5628935: Non-diseased nasopharyngeal organoids; Homo sapiens; RNA-Seq
Organoids were washed once with adDMEM/F12 to eliminate matrigel and then incubated with TrypLE express for 10 mins. Single cell suspensions were next achieved by passing through the large undissociated structures with a P1000 tip repeatedly. Single cells were centrifuged and resuspended in AO medium for cell counting to obtain a density of 1000 cells/µl. 10X Genomics Next GEM Single-Cell RNA v3.1