GSM5627970: attp2_5wk_3 (RNA-seq); Drosophila melanogaster; RNA-Seq
Hearts were exposed and removed, put in Quizol reagent before flash freezing and storing at -80. Subsequently RNA was harvested using Qiagen miRNeasy extraction kit. TruSeq Stranded mRNA Library Prep Kit High Throughput was used with total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols