GSM5600646: EGR1 CHIP-seq CD34 HSPC-3; Homo sapiens; ChIP-Seq
RNA was isolated from CD34+ HSPCs with Trizol. ChIP samples were generated from isolated HSPC nuceli using the EGR1 specific antibody 588 from Santa Cruz (sc-110) RNA libraries were constructed using Illumina's TruSeq Stranded mRNA kit. DNA libraries for ChIP seq were constructed using Tecan's Ovation DNA library preparation kit.