GSM6285641: KO, oxygen-glucose deprivation, replicate 3; Mus musculus; RNA-Seq
RNA (1 μg) from primary Cort−/− and Cort+/+ endothelial cell cultures (three independent biological replicated experiments each, with RNA Integrity Number coefficients >9, using Bioanalyzer RNA 6000 Nano-chip, from Agilent, Santa Clara, CA, USA) RNA was used to prepare mRNA libraries with TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA, cat#20020595). Quality and size distributions of indexed mRNA libraries were validated by Bioanalyzer High Sensitivity DNA assay (Agilent), and the final libraries were pooled equimolecularly and diluted/denatured as recommended. The 40x2nt paired-end sequencing was conducted on an Illumina NextSeq-500 sequencer (highest output mode), producing 46,850,000 raw paired-reads on average