RNA-seq for HS-3
High-quality RNAs were reverse transcribed into cDNA and then sonicated to the fragment size of 300500 bp. The RNA-seq libraries were constructed using the TruSeq Stranded mRNA Sample Preparation kit (Illumina, San Diego, CA, USA) following the manufacturers instructions and sequenced on an Illumina HiSeq X Ten system (Illumina, San Diego, CA, USA) at Shanghai OE Biotech Co., Ltd. (Shanghai, China)