scRNA-seq of femoral head
GemCodeTM Single Cell platform (10X GenomicsPleasanton, CA) was used to determine the transcriptomes of single cells.15 l of single-cell suspension at a concentration of ~900,000 cells/ml was loaded into one channel of the ChromiumTM Single Cell B Chip (10X Genomics1000073), aiming for a recovery of 8000-9000 cells. The Chromium Single Cell 3Library & Gel Bead Kit v3 (10X Genomics, 1000075) was used for single-cell barcoding, cDNA synthesis and library preparation, following manufacturers instructions according to the Single Cell 3Reagent Kits User Guide Version 3. Libraries were sequenced on Illumina novaseq 6000 using paired-end 155 bp.