GSM4792083: Repolarization: 2h M2→M0-depolarized mouse BMDMs, bulk RNA-seq, biological replicate 1; Mus musculus; RNA-Seq
For bulk RNA-seq, trizol followed by phenol extraction of bulk RNA. For single-cell RNA-seq, 10X Genomics single cell gene expression protocol following manufacturer recommendations. For bulk ATAC-seq, nuclei extraction and DNA tagmentation was performed as described in Buenrostro et al. (2013) "Transposition of native chromatin for fast and sensitive epigenomic profiling of open chromatin, DNA-binding proteins and nucleosome position." Nature Methods 10, 1213-18. For bulk RNA-seq, libraries were prepared using the Illumina TruSeq kit, following manufacturer recommendations. For single-cell RNA-seq, 10X Genomics single cell gene expression protocol following manufacturer recommendations. For bulk ATAC-seq, library prep was performed as described in Buenrostro et al. (2013) "Transposition of native chromatin for fast and sensitive epigenomic profiling of open chromatin, DNA-binding proteins and nucleosome position." Nature Methods 10, 1213-18.