GSM5129066: RNA-seq_pEF-1; Sus scrofa; RNA-Seq
Total RNA was extracted using the RNeasy Mini Kit (Qiagen, Cat.N: 74106) We used an rRNA depletion protocol (Globin-Zero Gold rRNA Removal Kit, Illumina, Cat.N: GZG1224) coupled with the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, Cat.N: E7420S) to construct the strand-specific RNA-seq library for each sample. All libraries were quantified using the Qubit dsDNA High Sensitivity Assay Kit (Invitrogen, Life Technologies, Cat.N: Q32851) and sequenced on HiSeq 4000 platform (Illumina) to produce an average of ~127.83 million 150-bp paired-end raw reads and ~126.01 million high-quality reads for each library.