GSM5014640: RNAseq_GM_sgNT_DMSO_rep1; Homo sapiens; RNA-Seq
For RNAseq, RNA was extracted in TRIzol using Quick-RNA Miniprep Kit following the manufacturer's protocol with on-column Dnase digestion (Zymo Research). For RNAseq, at least 100ng RNA was used to prepare the RNA-seq library using TruSeq® Stranded mRNA Library Prep Kit ( Cat# 20020594, Illumina) for each sample following the manufacturer's instruction.