GSM4971156: Tissue Anterior Brainstem GCGR-NS19 - 19.3 pcw; Homo sapiens; RNA-Seq
total RNA was extracted with Qiagen Rneasy plus mini kit Libraries were generated with 200ng of RNA input using the KAPA mRNA HyperPrep kit and KAPA SeqCap adapters. Fragmentation was carried out at 94°C for 6 minutes and libraries amplified for 12 cycles Pooled libraries were diluted and processed for sequencing using the Illumina HiSeq 2500 platform.