GSM5628972: A549 + Cas13d+NT5 2; Homo sapiens; RNA-Seq
Total RNA was prepared from transfection experiments after 48 h using a Qiagen RNeasy Plus Mini kit. mRNA was then extracted using a NEBNext Poly(A) mRNA Magnetic Isolation Module and RNAseq libraries were prepared using a NEBNext Ultra Directional RNA Library Prep Kit for Illumina sequencing. RNA-seq libraries were sequenced on an Illumina NextSeq instrument with at least 30 million reads per library. RNA libraries were prepared for sequencing using standard Illumina protocols