Sequencing of Arabidopsis thaliana mutant reca1/atrnh1c
Total DNA was extracted from ~100 mg pools of three-week-old plants using the Hi-DNAsecure Plant Kit (Tiangen) according to the manufacturers instructions. The DNA quality was assessed on a Nanodrop 2000 (Thermo Fisher Scientific) and the concentration was determined by Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific). Five nanograms of DNA was used to prepare the library with TruePrep DNA Library Prep Kit V14 for Illumina (Vazyme). The sequencing was performed on an Illumina HiSeq X Ten instrument.