NextSeq 550 sequencing; Human-Mouse Mixture experiment to estimate that contribution of Barcode swapping
Human-Mouse Mixture experiment to estimate that contribution of Barcode swapping
Cultured Cells were harvested and lysed in 100 µl RLT plus lysis buffer containing 1% beta-mercapto Ethanol and frozen at -80 °C. RNA was extracted using Zymo micro Prep Kit The prime-seq protocol was performed as described here: https://www.protocols.io/view/prime-seq-s9veh66