GSM5628873: HCT_GM12878_RNA_polymerase_II_Rep2; Homo sapiens; OTHER
HiC was carried out using the in-situ method as described previously (Rao et. al., 2014) with minor modifications.  In brief, cells were digested for 2 hours at 37C using 50U of DpnII. After biotin filling, proximity ligation was carried out for 2-4 hours at room temperature with 200U T4 DNA Ligase. After proximity ligation nuclei were then proceeded for Cut&Tag protocol as decribed previously (Kaya-Okur., et al, 2019) Library protocol was included in the CuT&Tag assay as decribed earlier (Kaya-Okur., et al, 2019).