B73-2_1-infected
Total RNA was ex-tracted from 100 mg of frozen tissue using TRIzol reagent (ThermoFisher, Waltham, MA, USA) according to the manufacturers instructions. Concentration of the resulting total RNA was determined with a Nanodrop 2000 and 4 biological replicates of each sample at the same concentration were mixed. mRNA was purified from the 12 total RNA pools using Dynabeads (Invitrogen, MA, USA) according to manufacturers instructions. A total of 12 paired-end (2  150) RNA-Seq libraries (six treatments, two replicates per treatment) were prepared and sequenced at the Beijing Genomics Institute facilities with DNBSEQTM technology