RNAseq of Lens culinaris
TruseqTMRNA sample prep Kit (Illumina, Inc. San Diego, CA, USA) was used for preparing cDNA library and two hundred base pair (bp) size inserts were used for post cDNA preparation following standard manufacturerss protocol. These fragments were ligated with Illumina adapters after repairing their ends. The PCR amplified library was analyzed in Tape Station 4200 (Agilent Technologies) (automated electrophoresis tool) using High Sensitivity (HS) D5000 Screen Tape assay kit as per manufacturer's instructions. After obtaining the Qubit concentration for the library and the mean peak size from Agilent Tape Station profile, the PE Illumina library was loaded for cluster generation and sequencing.