RNAseq of Prymnesium parvum UTEX2797: Low phosphorous
Cultures were kept at 20C using a 12:12 light dark cycle in 200 mol2 of light. Modified L1-Si growth media was used at 31 PSU; monosodium phosphate was limited to 1/25 the standard amount. Cells were harvested 6 hours into the light cycle (T = 6 hr) after the culture had reached approximately one million cells/mL. Stranded RNAseq libraries were prepared with NEBNext Ultra TM RNA Library Prep Kit fors Illumina (NEB, USA) following manufacturer's recommendations