GSM6214078: Spinal 1; Homo sapiens; RNA-Seq
Nuclei were isolated using a Nucleus Isolation Kit (catalogue no. 52009-10, SHBIO, China) according to the manufacturer's instructions. Nuclei suspension was loaded onto a Chromium single cell controller (10x Genomics) to generate single-nucleus gel beads in the emulsion (GEM) using single cell 3' Library and Gel Bead Kit V3.1 (10x Genomics, 1000075) and Chromium Single Cell B Chip Kit (10x Genomics, 1000074) according to the manufacturer's protocols. The 10x snRNA-seq library was established using Single Cell 3' Library and Gel Bead Kit V3.1, according to the manufacturer's instructions.