GSM5900283: purified plasma cells from the myeloma patient RD192 cultured and treated with vehicle; Homo sapiens; RNA-Seq
For scRNA-seq, sample preparation and sequencing were performed at MD Anderson Cancer Center's Advanced Technology Genomics Core. Sample concentration and cell suspension viability were evaluated using a Countess II FL Automated Cell Counter (Thermo Fisher Scientific). Samples were normalized for input onto the Chromium Single Cell A Chip Kit (10X Genomics, Pleasanton, CA), and single cells were lysed and barcoded for reverse transcription.