GSM5862305: ATAC-seq_ctl rep 2; Mus musculus; ATAC-seq
50,000 RAW 267.4 macrophages were lysed in the presence of 10 mM NaCl 0.1% IGEPAL CA-630. Tagementation was performed usign TDE1 transposase as per manufacturer recommendations.Tagmented DNA was prepared using Zymo-spin concentrator 5 columns Purified tagmented DNA was amplified with KAPA HIFI polymerase (12 PCR cycles) and Unique Dual Primers from Illumina (Cat number 20332088). Size-selection (L:1.1; R :0.6) was performed with KAPA Pure beads and size distribution of the final libraries was assessed on bioanalyzer (Agilent). Libraries were quantified by qPCR and loaded equimolarly on a S4 Novaseq flowcell. Each library was sequenced with a coverage of 50M paired-end reads (PE100).