scRNA-seq of hiPSC-derived neuronal cells, passage 5
Neural cells for analysis were harvested using a standard protocol using Accutase (Gibco). Suspension of the cells were prepared in DPBS (12,000 cells in 20 l DPBS for a single reaction). Libraries were made with 10x Genomics Chromium Single Cell 3' Reagent Kit (Chromium Single Cell 3 GEM, Library & Gel Bead Kit v3) according to manufacturer instructions.