GSM5133486: BCO5-3D; Homo sapiens; RNA-Seq
Total RNA was collected after removal of the culture medium by adding 700µl of QIAzol lysis reagent (Qiagen) to a 24 well containing organoids (two 25µL BME/matrigel domes). RNA was isolated using the miRNeasy kit (Qiagen) according to the manufacturer's protocol16 and the concentration was measured using a NANOdrop 2000 (ThermoFisher) 500 ng of total RNA was used for library construction using KAPA RNA Hyper + RiboErase HMR kit (Roche 08098140702)