Burkholderia pseudomallei Genome sequencing
One round of DNA size selection using SPRI-bead was performed to retain fragments larger than 6kb. Approximately 1,000 ng of size-selected DNA as measured by (Denovix, Wilmington DE) was directly used for library preparation using the LSK110 kit. The prepared library was sequenced on a Flongle and basecalling used Guppy v5.0.7 super accuracy model. The raw fastq readswere length-filtered to retain reads that are longer than 2 kb followed by de novo assembly using the Flye v2.9 --nano-hq mode. The Flye genome assembly was subsequently polished with one round of RACON and then one round Medaka, generating the final consensus assembly that consists of 2 circular and closed contigs. Genome completeness was subsequently assessed using BUSCO5 based on the Burkholderiales_odb10 lineage database.