RNAseq of resistant An. coluzzii
At 10am, 3-5 day old presumed-mated adult females were snap frozen in the -80oC, 5 individuals were used for each of the 4 biological replicates. RNA was extracted using a Picopure kit after homogenisation with a motorised pestle as previously described. Quality and quantity of the RNA was then analysed using an Agilent TapeStation and Nanodrop respectively and sent for sequencing at Centre for Genomics, Liverpool, UK.