RNA-seq of Medicago truncatula roots not inoculated with arbuscular mycorrhizal fungi, but harvested at 7 weeks post-inoculation
Extracted RNAs were treated with TURBO DNase (Thermo Fisher Scientific) and quantified using a NanoDrop ND-2000 spectrophotometer (Thermo Fisher Scientific). RNA quality was assessed using 2100 BioAnalyzer technology (Agilent Technologies). Approximately 100 ng of total RNA was used to construct poly(A) selection libraries using the Illumina TruSeq RNA Sample Preparation kit.