GSM4905400: H2B8_seedling_RNAseq_rep3; Arabidopsis thaliana; RNA-Seq
The Plant RNeasy mini kit (QIAGEN) was used to extract RNA from 10-day-old seedlings. RNA was converted to cDNA by RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific). Libraries were prepared with the Nugen Universal RNA-Seq library preparation kit (0364) and sequenced on the NextSeq 500 (Illumina) with 2 × 38 bp paired end reads at the John Innes Centre, Norwich, UK.