GSM5239341: empty plus multi-guide 2 (PDS) rep1; Nicotiana benthamiana; ncRNA-Seq
Leaf tissue from agroinfiltrated area were collected and flash frozen in liquid nitrogen. Total RNA was extracted using Trizol protocol. DNBseq Small RNA Library, BGI Tech Solutions. Breifly, total RNA is size seperated (18-30nt) using PAGE and size selection. Adapters are ligated and first strand synthesis following BGI protocol. Libraries were PCR amplified, size selected and sequenced using the DNBseq platform.