GSM5945833: STRT_12.H1.8c.DOXplus; Homo sapiens; RNA-Seq
The libraries were prepared using the STRT method as described in Krjutskov et al., Hum. Reprod. 2016 with minor modifications. Briefly, RNA samples were placed in a 48-well plate in which a universal primer, template-switching oligos, and a well-specific 8 bp barcode sequence were added to each well (Krjutskov et al., Hum. Reprod. 2016). The synthesized cDNAs from the samples were then pooled into one library and amplified by single-primer PCR with the universal primer sequence. The resulting amplified library was then sequenced using an Illumina NextSeq500 instrument. RNA-seq (Bulk STRT)