Microbial community characterization of  Ozonated water passing through a BAC filter (20 mins)
The sequencing data of the 16S rRNA amplicons were processed following the analysis pipeline of RTL Genomics. In brief, paired-end reads were trimmed and merged using PEAR Illumina, then denoised, and chimera checks were performed before the downstream process. The number of sequences per sample ranged from 12089 to 39366. Chimera detection and removal were done by using UCHIME in de novo mode after denoising the data. To obtain the identity of each remaining sequence, samples were demultiplexed. Then using the UPARSE algorithm (Edgar, 2013), sequences were clustered into operational taxonomic units, OTUs. Taxonomic information is added to each sequence after running each cluster against the USEARCH global alignment program. The data is identified by employing a database of high-quality sequences derived from the NCBI database. Once sequencing data were obtained, all data processing related to microbial community analysis were made following different pipelines (Callahan et al., 2016; Shetty et al., 2018).