Neuron: Sample R00AL172
50,000 nuclei were aliquoted into the first step of the ATAC-seq published protocol (Buenrostro et al., Nature Methods, 2013). Libraries were prepared following the published instructions except that the last clean up step was modified to use KAPA pure beads (AmpureXP beads at a 1:1 ratio to remove dNTPs, salts, primers or primer dimers) instead of Qiagen Minelute clean-up kit. All libraries were sequenced to a minimum depth of 13.6M and an average depth of 25.5M using 50 bp PE sequencing on an Illumina HiSeq2500 or MiSeq machine.