GSM5708203: Male_PFC_Veh FTL_275; Mus musculus; RNA-Seq
Brain punches (n = 120) were homogenized in Trizol Reagent (Invitrogen, Cat, num. 15596018) and Zirconium Beads RNase Free (Next Advance, Cat. num. ZrOB05-RNA 0.5mm) using the Bullet Blender Blue (Next Advance, Model. num. BBX24B) at speed 6 for 1 min. RNA was then purified using the Direct-Zol kit (Zymo, Cat. num. R2052). RNA quality was assessed using the Agilent 2200 TapeStation system and samples with RIN above 7.5 were used for library preparations. An average of 200ng of RNA was used for each sample to prepare the cDNA libraries. The NEBNext Ultra II Directional RNA Library Prep Kit (New England BioLabs, Cat. Num. E7760S) was used following manufacturer's instructions. A total of 119 libraries were sequenced on the Illumina NovaSeq 6000 system (2 x 100 bp reads) polyA-enriched RNA-Seq