Illumina HiSeq 1000 sequencing; Monitor the efficiency of "WIND: A Workflow for pIRNAs aNd beyonD" for the identification of piRNA in mouse samples
Monitor the efficiency of "WIND: A Workflow for pIRNAs aNd beyonD" for the identification of piRNA in mouse samples
Cardiomyocytes were isolated from C57BL/6J adult mice by enzymatic dissociation. RNA was extracted from aCMs, using TRIzol Reagent (Ambion) and quantified. The libraries were generated using depleted RNA obtained from 1 µg total RNA by TruSeq Sample Preparation RNA Kit (Illumina, Inc., San Diego, CA, USA) according to the manufacturer's protocol without further modifications