RNA-seq of Anopheles coluzzii adult female antenna
To create the antennal RNA-seq libraries, we removed the whole antenna from the base of the pedicel and isolated total RNA using TriZol purification methods. The tissues were disrupted and homogenized using a power pestle with disposable RNase free pestles. Total RNA samples were stored at -80C and shipped to Genewiz Inc, where they were first assessed for quantity (Qubit Quantification) and quality (Agilent 2100 Bioanalyzer). RNA library preparation with polyA selection was then carried out using the Illumina HiSeq with a 2x150bp configuration.