GSM5207283: Batch I (S2) TCR; Homo sapiens; RNA-Seq
Live bulk CD8 T cells were sorted out and processed according to 10x Genomics Chromium protocol 10x genomics kits were used to produce the Gel Bead-In Emulsions (GEMs) and the resulting sequence libraries (Gene expression library, Feature Barcode library, TCR library) according to manufacturer's protocol: For batch I, Chromium Next GEM Single Cell 5' Library and Gel Bead kit v1.1 (10x Genomics, PN-1000167), Chromium Next Gem Single Cell V(D)J Reagent Kit v1.1 (10x Genomic, PN-1000165), Chromium Single Cell 5' Library Construction Kit (10x Genomics, PN-1000020), Chromium Single Cell 5' Feature Barcode Library Kit (10x Genomics, PN1000080) and Chromium Next GEM Chip G Single Cell kit (10x Genomics, PN-100127). For batch II, Chromium Next GEM Single Cell 5' Kit v2 (10x Genomics, PN-1000265), Chromium Next GEM Single Cell 5' Reagent Kits v2 (Dual Index) (10x Genomic, PN-2000263), Library Construction Kit (10x Genomics, PN-1000190), 5' Feature Barcode Kit (10x Genomics, PN-1000256) and Chromium Next GEM Chip K Single Cell kit (10x Genomics, PN-1000287). The 3 libraries were combined in relative fractions of 0.785, 0.085 and 0.130 in order to generate sufficient reads per cell for each type of library.