AVENIO Surveillance on 20 ng synthetic human reference  standard: Seraseq ctDNA Mutation Mix v2 AF0.125%
DNA was prepared into libraries using  the Roche AVENIO surveillance kit according to manufacturer's  recommendations. Library was sequenced on Illumina NextSeq 500 and analyzed on the AVENIO Oncology  Analysis Server. Deduped bam used for SNV calls. PosDeduped bam used for  INDEL calls. Sorted bam used for CNV and fusion calls.