GSM5946239: PF-nSene-1; Mus musculus; RNA-Seq
Total RNA was extracted using RNeasy Mini Kit (QIAGEN) according to the manufacturer's instructions. Removal of rRNA from total RNA (1 mg) was carried out using NEBNext rRNA Depletion Kit (New England Biolabs). RNA-seq libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs). After the conversion of the linear dsDNA libraries to single-stranded circular DNA libraries (Universal Library Conversion Kit, MGI Tech, Guangdong, China), the pooled libraries were sequenced on a DNBSEQ-G400RS (MGI Tech) using paired-end mode (2×100 bp).