GSM5920742: ELK3KD-231 +Flag-ELK3_Input; Homo sapiens; ChIP-Seq
ELK3KD-231 cells were transfected with pLenti-cMyc-Flag-ELK3. After 48hrs, cells were fixed in 1% paraformaldehyde solution to cross-link proteins and genomic DNA. Glycine was added to quench the formaldehyde. After washing, cells were lysed using cell lysis buffer. lysates were sonicated to fragment genomic DNA and then centrifuged. The supernatants were mixed with protein A/G magnetic beads and incubated with anti-Flag antibody. After washing, proteinase K and SDS were added to separate the DNA-protein complexes. Immunoprecipitated genomic DNA was purified using the phenol/chloroform extraction method. The construction of library was performed using NEBNext®UltraTM DNA Library Prep Kit for Illumina (New England Biolabs, UK) according to the manufacturer's instructions.