GSM5130004: late endorsperm stage endorsperm RNA-seq [LEN-1]; Brachypodium distachyon; RNA-Seq
Embryo, endosperm and seed coat isolations were performed using a dissecting microscope, fine forceps and needles in a 4.8% Sucrose solution that contained 0.1% RNALater solution.For seed coat isolation, the embryos were manually removed, and the remaining seed coat was kept. Total RNA was extracted from each stage embryo sample following the protocol of RNAqueous-micro kit. RNA-seq libraries were prepared using TruSeq RNA kit (version 1, rev A).