Genomic sequencing of rhabdomyosarcoma/Genomic Classification and Clinical Outcome in Rhabdomyosarcoma: A report from an International Consortium. : Sample RMS2450
"DNA was extracted from FFPE tumor tissue using AllPrep DNA/RNA FFPE kits (Qiangen, Germantown). One hundred nanograms of DNA was sheared to approximately 200 base pairs (bp) by sonication (Covaris, Woburn, MA). Exome enrichment was performed using SureSelect Clinical Research Exome Kits according to the manufacturer's instructions (Agilent, Santa Clara, CA) . Paired-end sequencing (2X75 bp) was performed on an Illumina NextSeq500 instrument."