GSM4970481: DN3_FA_GATA3_ChIP-rep2; Mus musculus; ChIP-Seq
5-10M cells were fixed with 1 mg/ml DSG (Thermo Scientific) in PBS for 30 min at RT followed by an additional 10 min with addition of formaldehyde up to 1%, or with 1% FA formaldehyde for 10 min. Five ug of anti-GATA3 mAbs (a mixture of 2.5 ug of sc-268, Santa Cruz and, 2.5 ug of MAB26051, R&D systems) were pre-bound to Dynabeads anti-Mouse Ig (Invitrogen) and then added to the diluted chromatin complexes in parallel aliquots. Precipitated chromatin fragments were cleaned up using Zymo ChIP DNA Clean & Concentrator. ChIP-seq libraries were constructed using NEBNext ChIP-Seq Library Preparation Kit (E6240, NEB) and sequenced on Illumina HiSeq2500 in single read mode with the read length of 50 nt.