MicroRNAs-seq of tea leaves uninfected by Epicoccum nigrum for 48 h
Sterile PDA was used to inoculate the tea leaves at 25C for 48 h before tea leaves harvested, ground with liquid nitrogen. About 100 mg powder was used for RNA extraction.The libraries of miRNA sequencing were prepared using TruSeq Small RNA Sample Prep Kits (Illumina, San Diego, USA) following the manufacturer's procedure. The libraries were sequenced on an Illumina Hiseq 2500 (LC Sceiences, USA) at the following the vendors recommended protocol,then following the manufacturer's procedure.