RNAseq of Prymnesium parvum UTEX2797: Standard T12
Cultures were kept at 20C using a 12:12 light dark cycle in 200 mol2 of light. L1-Si standard growth media was used at 31 PSU. Cells were harvested at the beginning of the dark cycle (T = 12 hr) after the culture had reached approximately one million cells/mL. Stranded RNAseq libraries were prepared with NEBNext Ultra TM RNA Library Prep Kit fors Illumina (NEB, USA) following manufacturer's recommendations