Negative feedback: Replicate6
Isolates were passaged for at least twice after the long term cultures, before harvested for genomic DNA extraction. The extracted DNA were then used to amplified the region from the EF1a promoter to the polyA sequence (position 824-8949 on the plasmid map),. Sequencing library were prepped and indexed with Illumina Nextera XT kit and sequenced on Illumina MiSeq, with 300nt pair-ended setup.