Guano addition experiment March T1 5x No Anti
guano addition experiment 5x without antibiotics incubated during 72h .Microbial community DNA retained in 0.2 m Sterivex was extracted with Power Water isolation kit (Qiagen) following the manufacturers instructions. DNA sample concentration was quantified using a Qubit 2.0 fluorometer and the Qubit dsDNA HS Assay Kit (Thermo Fischer Scientific Inc., Massachusetts, USA). The region V4 from the 18S rRNA of eukaryotic was amplified using the primers TAReuk454FWD1 and TAReukREV3 (Logares et al., 2014).