GSM5324098: RNAi-MTA_IP_Rep1; Fragaria x ananassa; RIP-Seq
Total RNA was extracted with the plant RNA extraction kit (Magen, R4165-02) from the MTA RNAi strawberry fruits and the controls, respectively. mRNA was isolated from the total RNA and then fragmented, followed by immunoprecipitation with specific anti-m6A antibody (Synaptic Systems, 202003). For library construction, 50 ng of immunoprecipitated mRNAs or pre-immunoprecipitated mRNAs (input control) were used for library construction with NEBNext ultra RNA library prepare kit for Illumina (NEB, E7530). High-throughput sequencing was performed on the Illumina HiSeq X sequencer with a paired-end read length of 150 bp according to the standard protocols.