18S rRNA gene amplicon of bovine: rumen liquid
Rumen samples were collected by using stomach tube. Samples were aliquoted, snap frozen in dry ice and stored at -80 oC until DNA extraction. Total DNA was extracted as described by Rius et al. (2012). For microbial amplicon sequencing  primers targeting protozoal 18S rRNA gene region were used (Sylvester et al., 2004).