Illumina NovaSeq 6000 sequencing; Mutant FUS triggers age-dependent synaptic impairment in presymptomatic ALS-FUS mice (RNA-seq part2)
Mutant FUS triggers age-dependent synaptic impairment in presymptomatic ALS-FUS mice (RNA-seq part2)
Hippocampal and cortical primary neurons were prepared from E17 embryos. Cells were treated with Actinomycin D at DIV 12 for 24h,12h,8h or 0h (not treated). Primary neurons from one embryo were seeded on four wells, one for each Actinomicin D treatment time point. Primary neurons were collected at the same time and total RNA was extracted using RNeasy Plus Mini Kit (Cat No. 79254, Qiagen). Hippocampal and cortical primary neurons were prepared from E17 embryos. Cells were treated with Actinomycin D at DIV 12 for 24h,12h,8h or 0h (not treated). Primary neurons from one embryo were seeded on four wells, one for each Actinomicin D treatment time point. Primary neurons were collected at the same time and total RNA was extracted using RNeasy Plus Mini Kit (Cat No. 79254, Qiagen). 150ng of total RNA were used for Poly A library preparation. Strand specific cDNA libraries were prepared.