GSM5939815: polysome_MF_rep1_RNA_BS_seq; Mus musculus; OTHER
Total RNA was extracted from adult NSCs with TRIzol reagent. Polysome fractions were separated by sucrose gradient separation and polysome RNA was extracted from polysome fractions with TRIzol LS reagent following the manufacturer's instructions. RNA samples were subject to turbo DNase digestion at 37℃ for 30min. After two rounds of poly(A) selection, bisulfite conversion was performed and RNA-seq libraries were prepared for sequencing using standard Illumina protocols RNA BS-Seq