MeRIP-seq of Col-0 3h flower Input biorep1
Total RNA was isolated using the Trizol reagent (Invitrogen) and polyA RNA was purified using  Oligo (dT) 25 magnetic beads (Thermo Fisher). For m6A sequencing 50 g total RNA were used. Poly(A) RNA was fragmented into small pieces using Magnesium RNA Fragmentation Module (NEB, cat.e6150, USA) under 86 7min. Libraries preparation was performed using NEBNext Ultra Directional RNA Library Prep Kit for Illumina following the manufacturers instructions. For IP sequencing, the cleaved RNA fragments were incubated for 2h at 4 with m6A-specific antibody (No. 202003, Synaptic Systems, Germany) in IP buffer (50 mM Tris-HCl, 750 mM NaCl and 0.5% Igepal CA-630). 150bp paired-end sequencing (PE150) were done either on an Illumina Novaseq 6000 or Illumina Hiseq XTen platform.