LIBRA-seq with epitope mapping on PBMCs from an HIV-1 infected human donor
LIBRA-seq with epitope mapping on PBMCs from HIV-1 infected human donor samples. Human PBMCs were stained with fluorescently labelled antibodies and oligo-tagged HIV-1 and control antigens, sorted via FACs, and processed for single cell sequencing with the 10X Genomics Chromium Controller (5'VDJ kit with antigen barcode processing). The VDJ and antigen libraries were sequenced on the Novaseq (Illumina)