GSM5600633: SB02_10-d2-7 (low lithium); Homo sapiens; RNA-Seq
Total RNA was extracted using the Qiagen AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Hilden, Germany). All RNA samples were of good purity and integrity, as indicated by 260/280 ratios of between 1.95 to 2.1, tested using a Nanodrop D1000 spectrophotometer (Thermo Scientific, Wilmington, DE), and RINs > 9, as tested using an Agilent 2100 Bioanalyzer (Agilent Technologies, Berkshire, UK). Library preparation and RNA-sequencing was performed at The Genomic Centre, King's College London. Briefly, total RNA samples were submitted to a DNase treatment using the DNA-free™ DNA Removal Kit (Invitrogen, California, USA). Subsequently, 300 ng of total RNA from each sample was submitted for ribosomal RNA depletion using the NEBNext rRNA Depletion kit (New England Biolabs, Massachusetts, USA), and RNA-Seq libraries were constructed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). The samples were sequenced in a HiSeq 4000 sequencing system (Illumina).