GSM5946005: LGE Isl1 cKO replicate 4; Mus musculus; RNA-Seq
1 ug of total RNA was used to isolate polyA RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England BioLabs). Samples were further enriched using Apollo NGS library prep system (Takara Bio USA). The library was prepared using the NEBNext Ultra II Directional RNA Library Prep kit (New England BioLabs) and quantified with the NEBNext Library Quant kit (New England BioLabs). Following proportional pooling of individually indexed libraries, sequencing (single read 1x85 bp) was performed using a NextSeq 550 sequencer (Illumina)