BGISEQ-500 paired end sequencing; Bulk segregant analysis of 3D growth defective mutant in Physcomitrella patens (nog2)
Bulk segregant analysis of 3D growth defective mutant in Physcomitrella patens (nog2)
Protonemal filaments maintained on ABCD medium prior to harvest of genomic DNA. 3D defective growth mutants were generated by UV mutagenesis of protoplasts derived from the Vx::mCherry line (Villersexel strain that accumulates mCherry). Protonemal filaments maintained on ABCD medium Genomic DNA was extracted using CTAB protocol Genomic DNA fragmented, repaired, A-tailed and adaptor ligated before size selection and amplification