BGISEQ-500 paired end sequencing; Whole genome sequencing in cohort of probands with isolated cleft palate
Whole genome sequencing in cohort of probands with isolated cleft palate
Blood samples were drawn from recruited individuals. Total chromosomal DNA was extracted from peripheral blood leukocytes using the standard phenol/chloroform method. Libraries were prepared from 300 ng of high-quality genomic DNA using a MGIEasy Universal DNA Library Prep Set (MGI Tech Co., Shenzhen, China), according to the manufacturerâ€™s instructions.