16s V3-V4 amplicon sequencing of human gut microbiome
DNA was extracted from human stool samples using the Qiagen Powersoil kit per manufacturers instruction. The V3-V4 region of the rRNA gene was amplified and Illumina libraries generated as described previously (Jensen et al, Biotechniques 2019). Libraries were sequenced on an Illumina MiSeq using 2 x 300 bp paired end sequencing.