WGS : Vibrio cholerae clinical isolate
DNA was quantified by QUBIT 3 Fluorometer using dS DNA HS Dye. 100 nanograms of intact DNA was enzymatically fragmented by targeting 200- 300 bp fragments size. The samples are further purified using AMPure beads and The DNA is then enriched by PCR with 6 cycles usingNEBNext Ultra II Q5 master mix, illumina universal primer and sample- specific octamer primers.The amplified products are cleaned up by using AM pure beads and the final DNA library was eluted in 15 uLs of 0.1X TE buffer. 1ul of the library was used to quantify by QUBIT 3 Fluorometer using dS DNA HS reagent. The fragment analysis was performed on Agilent 2100 Bioanalyzer,by loading 1 uL of the library in to Agilent DNA 7500 chip.