RNA-Seq of Longissimus dorsi muscle: Xianan steers
Six RNA libraries for each Longissimus dorsi muscle sample were built. Oligo (dTs) were used to isolate poly (A) mRNA. The mRNA was fragmented and reverse transcribed using random primers. The double-stranded cDNAs were synthesized using RNase H and DNA polymerase I. Te double-strand cDNAs were then purified using the QiaQuick PCR extraction kit. The required fragments were purified via agarose gel electrophoresis and were enriched through PCR amplification. Finally, the amplified fragments were sequenced using Illumina HiSeq 2000 according to the specifications of manufacturer.