GSM5324394: 9_Serum_4Gy; Homo sapiens; miRNA-Seq
Blood samples were collected at pre-specified timepoints via venipuncture. Samples were centrifuged and frozen to -80oC within 4 hours of collection. Exosomes were isolated from selected serum samples using the Qiagen miRCURY Exosome Serum/Plasma Kit. Total RNA was isolated using the Qiagen miRNeasy Serum/Plasma Kit. After RNA extraction, small RNA-seq (next generation sequencing) indexed sequencing libraries were prepared from 200 ng total RNA according to QIAseq miRNA Library Kit Handbook (version 11/2016).