WGS from human tumor
DNA and RNA were extracted from tumor samples using the Qiagen AllPrep kit. . DNA sequencing libraries were prepared with the KAPA Hyper Prep kit, and samples were sequenced to a target depth of 60x coverage (for tumor) or 30x coverage (for saliva) on an Illumina NovaSeq. RNA isolations with RIN > 7 were prepared with the KAPA Stranded RNAseq with RiboErase kit and sequenced on an Illumina HiSeq or NovaSeq to a target of 200 million 150bp paired reads