Shotgun metagenomics of the Socompa's stromatolite
Metagenomic libraries (n = 6) were prepared through the use of the Nextera XT DNA Sample Preparation Kit, according to the manufacturers instructions. The DNA of those libraries was purified with AMPure XP beads and quantified by the fluorimetric Qubit dsDNA High Sensitivity Assay system (Life Technologies). The quantification of the libraries was performed with the 7500 Real Time PCR (Applied Biosystems, Foster City, CA, USA) and the KAPA Library Quantification Kit (Kapa Biosystems, Wilmington, MA, USA). The library-size distribution was accessed with the 2100 Bioanalyzer (Agilent). The six metagenomes (one per layer) were sequenced by Illumina MiSeq paired-end sequencing (2 x 150 base pairs) at the Laboratory of Microbiology (Federal University of Rio the Janeiro).