the 2-day natural restoration group_rep1
After a one-week adaptation, male C57BL/6 mice were randomized by body weight into six experimental groups. Mice in the control group were given normal drinking water, and mice in the other five groups were given metronidazole (1 mg per mL) in drinking water for one week. After this modelling period, the 2-day anthocyanin restoration group and the 5-day anthocyanin restoration group received bilberry anthocyanin extracts (50 mg per kg body weight) via oral gavage every day, while the 2-day natural restoration group and the 5-day natural restoration group received isovolumetric sterile water. Genomic DNA was extracted from the colon content sample using the QIAamp DNA Stool Mini Kit according to the manufacturers instructions. The V3V4 hypervariable region of 16S rRNA was amplified with universal primers 338F and 806R by PCR.