GSM4906375: primary mouse epidermal keratinocytes_P0_Sas-4_ mutant_1; Mus musculus; RNA-Seq
For epidermal keratinocyte isolation, back skin of P0 animals was dissected and incubated overnight at 4°C with dispase II to remove the dermis from the epidermis. Then, epidermal sheets were treated with TrypLE solution to dissociate the keratinocytes. Samples were frozen until further RNA isolation. RNA isolation was performed by following the manufacturer's instructions of the Quiagen RNeasy Mini Kit. Total RNA samples were used for RNA-Seq analysis. RNA libraries were prepared for sequencing using standard Illumina protocols