GSM5946260: Vero E6 uninfected control rep1; Chlorocebus sabaeus; RNA-Seq
Total RNA was extracted using a total RNA miniprep kit (Sigma) with the On-column DNase I digestion step included. Libraries were prepared from isolated total RNA using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina from Lexogen (Vienna, Austria). Barcoded libraries were pooled, and single end sequenced (1X75) on the Illumina NextSeq 500 system using the High output V2.5 kit (Illumina Inc., San Diego, CA). Read data was processed and multiplexed with the BlueBee Genomics Platform (BlueBee, San Mateo, CA).