Illumina HiSeq 2500 sequencing of SAMD00325718
Total RNA was isolated using Trizol followed by removing genomic DNA by DNase and examined by gel electrophoresis. Quality of total RNA was analyzed by Agilent Tape station 2200 The RNA Integrity Number  was determined for the sample before proceeding to RNA library construction.  The small RNA library was constructed from the RNA pools of samples. Total RNA was taken and preceded for library preparation using Truseq small RNA library prep kit as per the manufacturer instructions. Final Library was quantitated by Qubit 2.0 fluorometer and the quality was analyzed by Agilent Tape station 2200. Single end sequencing was performed on denatured libraries using rapid mode of HiSeq 2500 v3 chemistry