GSM4792196: TS576 cells in 3D soft hydrogel_rep1; Homo sapiens; RNA-Seq
Sphere cultured cells were dissociated using Accutase. 3D hydrogels were first digested with collagenase and hyaluronidase to release the cells, and the cells were dissociated with Accutase. RNA was harvested using Trizol reagent. RNA libraries were prepared for sequencing using standard Illumina protocols for Illumina NovaSeq 6000.