NextSeq 500 paired end sequencing; RNA-seq expression analysis of patient-derived xenografts in NSG mice--Dana-Farber Cancer Institute blood cancers
RNA-seq expression analysis of patient-derived xenografts in NSG mice--Dana-Farber Cancer Institute blood cancers
Collected by third party. mRNA was extracted by third party from mouse-depleted PDX leukemia and lymphoma cells using magnetic microbeads (uMACS mRNA Isolation Kit, Miltenyi Biotec). Non-stranded RNA-seq libraries were generated by third party usingÂ  the TruSeq RNA Sample Prep Kit (Illumina) on a Sciclone NGS platform (Perkin Elmer). mRNA underwent fragmentation, cDNA synthesis, and next-generation library synthesis via end repair, polyadenylation, adapter ligation, and PCR amplification.