JTNP Fungal (ITS) amplicon of Biocrust temoporal study
DNA extraction was performed with 0.15 g of biocrust using QIAGEN DNeasy PowerSoil kit (Qiagen, Germantown, MD, USA) following manufacture standard protocol. ITS1F and ITS primers set were used to amplify ITS1 region for fungal community following (Smith and Peay 2014).