GSM5923505: 449U; Mus musculus; RNA-Seq
Samples were lysed with RLT Plus buffer (Qiagen, 74134) with β-mercaptoethanol (1%) and homogenized with QIAshredder Spin Coumns (Qiagen, 79654). RNA was extracted using the RNeasy Plus Mini Kit (Qiagen, 74134), according to the manufacturer's protocol plus on-column DNase treatment using the RNase-Free DNase Set (Qiagen, 79254). mRNA libraries were prepared using NEBNext Ultra Kit with polyA selection (New England BioLabs).