RNA-Seq of Acropora surculosa: adult
Tissue samples were cut from coral nubbins and then immediately placed into RNALater (Sigma-Aldrich R0901) and stored at -80C on site. Total RNA was extracted using a Qiagen RNeasy kit (Hildenheim, Germany) on a QIAcube extraction robot. In total 36 cDNA libraries were constructed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs) Libraries were barcoded with individual indices from the NEBNext Multiplex Oligos for Illumina. Libraries were pooled into two sets of 18 samples and sequenced seperately using an Illumina NextSeq500 (San Diego, CA) with 75bp paired-end reads of 150bp.