miRNA-seq of Vitis vinifera
Total RNA was extracted from grapevine leaves using SpectrumTM Plant Total RNA Kit (Sigma-Aldrich) following manufacturers instructions. RNA purity and quality were tested in agarose gel electrophoresis. RNA samples were sent to Fasteris (https://www.fasteris.com/en-us/) (Switzerland) to perform small RNA sequencing on an Illumina HiSeq 4000 platform with 1x50 bp reads. Expected data output or 260-300 million reads per lane.