GSM5920673: MDSCs-siNC; Mus musculus; RNA-Seq
RNA was extracted using TRIzol (Life Technologies). Contaminating DNAs were removed using RNeasy spin columns (Qiagen). The quality of isolated RNA samples was evaluated with an Agilent Bioanalyzer 2100 (Agilent technologies) and the purified RNA was quantified using a NanoDrop ND-2000 spectrophotometer (Infinigen Biotech). RNA libraries were prepared for sequencing using standard Illumina protocols