GSM5945796: Seq01_Th1_4; Mus musculus; RNA-Seq
Cells were stained for flow cytometry and and sorted directly into Trizol LS. RNA was isolated using Maxwell RSC miRNA Tissue Kit (Promega) and purified using Agencourt RNAClean XP Beads (Beckman Coulter) Libraries were generated with the SENSE mRNA-Seq Lib Prep Kit V2 (Lexogen) using 100 ng RNA as input and with half of the volume as described in the manual. The PCR products were purified with Agencourt AMPure XP beads (Beckman Coulter)