Meta-taxonomy of V3 amplicon of 16S rRNA gene: fish gut
PCR amplification of V3 region for GCA was performed with domain-specific primers following the fusion primer protocol. To amplify the 16S rRNA genes we used universal forward primer 341f (5-CCTACGGGAGGCAGCAG-3) and universal reverse primer 515r (5- TATTACCGCGGCTGCTGG-3). DNA concentration was balanced to 26 pM within V3 amplicon pool using Bioanalyzer 2100 (Agilent Technologies). Sequencing was performed in an Ion S5 next-generation DNA sequencing machine.