Metagenome-Seq of SURF Sediments
The total genomic DNA from the SURF sample was extracted following the MINES protocol After checking the DNAs quality using a Nano-Drop 2000 Spectrophotometer (Thermo Fisher Scientific, United States), the DNA was stored at -20C, until further analysis. The following 16S rRNA gene amplification, library creation, Illumina 2X150-bp sequencing, and diversity analysis, were done using the services of RTL genomics (Lubbock, TX). During the procedure, the primer pairs used for analyzing bacterial diversity were 515F (5GTGCCAGCMGCCGCGGTAA3) and 806R (5GGACTACHVGGGTWTCTAAT3)