Whole-genome sequencing of a clone TDA1001 (Wild-type; ancestral) - tagged with sfGFP
Library construction and sequencing were performed at the genomics facility at Instituto Gulbenkian de Ciencia (Oeiras, Portugal). Nextera XT kit from Illumina was used for library construction. Briefly, each population was defrosted onto BHIS-Ery-Gen-agar and incubated anaerobically at 37C for 48h. DNA was then extracted from a single colony for whole genome sequencing on a MiSeq Benchtop Sequencer.