Illumina HiSeq 2500 sequencing of SAMD00325719
Total RNA was isolated using Trizol followed by removing genomic DNA by DNase and examined by gel electrophoresis. Quality of total RNA was analyzed by Agilent Tape station 2200. The RNA Integrity Number was determined for the sample before proceeding to RNA library construction.  The small RNA library was constructed from the RNA pools of samples. 1000ng of total RNA was taken and preceded for library preparation using Truseq small RNA library prep kit as per the manufacturers instructions. The final Library was quantitated by Qubit 2.0 fluorometer and the quality was analyzed by Agilent Tape station 2200. Single end sequencing was performed on denatured libraries using the rapid mode of HiSeq 2500 v3 chemistry.