GSM5628749: [Peripheral blood PCR] RDEB PB, 35 cycles #2; Homo sapiens; OTHER
gDNA was extracted from the epidermis, dermis, or peripheral blood by the Puregene Blood Core Kit (Qiagen, Valencia, CA, USA). Libraries were constructed using Ligation Sequencing Kit SQK-LSK109 and Native Barcoding Expansion kit EXP-NBD104 and EXP-NBD114 (Oxford Nanopore Technologies). gDNA was treated with Quick Calf Intestinal Phosphatase and Cas9 before library construction for Nanopore Cas9-targeted sequencing (nCATS).