Iso-Seq of tissues of O. sativa
RNA from leaves, panicles, inflorescences, roots, and seeds was pooled for cDNA library preparation. Iso-Seq cDNA (complementary DNA) library was prepared on 0.9g of pooled RNA, according to the Iso-Seq protocol of Pacific Biosciences (P/N 101-070-200 V. 06) using SMARTer PCR cDNA Synthesis Kit  (Pacific BioSciences of California, Inc., Menlo Park, CA, USA) and BluePippin Size-Selection System (Sage Science, Beverly, MA, USA)