GSM5239147: Donor 3, yd(2) Day 9; Homo sapiens; RNA-Seq
Cells were harvested, stained with an anti-yd TCR antibody and FACS sorted (BD FACSAria II Cell Sorter) for live yd TCR cells. Total RNA was isolated from the sorted live cells using TRIzol. Ribosomal RNA was removed using Ribo-Zero. PolyA isolation and library preparation were performed using NEBNext and the sample were run on Illumina HISeq platform.