RNAseq Male Exercise Participants - Sleep Study
approximately 1 _g total RNA was used for library construction. For library preparation, the poly(A)_+_mRNA molecules were purified using oligo (dT) magnetic beads. The mRNA was fragmented into small pieces and the double-strand cDNA was synthesized using N6 random primers. These cDNA fragments were added with a single A base and subsequently ligated to the adapter. The ligation product was purified and enriched with PCR amplification to yield the final cDNA library. The cDNA library was sequenced using BGISEQ-500 system with a paired-end sequencing length of 100bp. Raw RNAseq counts for each sample are available in Supplementary Table 1.