GSM5708084: 2L4_50; Rattus norvegicus; RNA-Seq
The rats were anesthetized with intraperitoneal injection of 2% pentobarbital sodium (0.2ml/100g) and then killed by visceral blood collection. An abdominal incision was made to expose the urinary bladder with stereoscope. The obtained bladder tissues were washed with pre-cooled isotonic normal saline and then cut into pieces. Washed away the visible clots, and removed the connective tissue, fat and pollutants. Removed the components likely to interfere the required targets. Centrifugated at 4℃, 1000 rpm for 2min, and removed the supernatant. Separated the tissue, one was for RNA extraction according to the specification of the RNA extraction kit. RNA libraries were prepared for sequencing using standard Illumina protocols