NextSeq 500 sequencing; Comparison of Tn-seq of Escherichia coli using B-2163 and MFDpir as donor strains.
Comparison of Tn-seq of Escherichia coli using B-2163 and MFDpir as donor strains.
Donor and recipient cells were conjugated and transposon insertion mutants were selected in plates with Km. Cells were collected from plates and gDNA was extracted. GenElute Bacterial Genomics DNA kit (Sigma) Library construction was paerformed as described in Espinosa et al 2020