Illumina HiSeq 4000 paired end sequencing; RNA-seq of human brain microvascular endothelial (hBME) cells infected with Toxoplasma gondii RH tachyzoite for 6, 24 and 48 hours versus control
RNA-seq of human brain microvascular endothelial (hBME) cells infected with Toxoplasma gondii RH tachyzoite for 6, 24 and 48 hours versus control
hBME cell were infected with T. gondii RH tachyzoite at MOI of 2 for 6, 24 and 48 hours.  Human brain microvascular endothelial cells (hBMECs) were grown in Gibco® Roswell Park Memorial Institute (RPMI) 1640 media (Thermo Fisher Scientific, Wal-tham, MA, USA) as described previously in Elsheikha, H.M.E., N.A.; Chan, K.L.A.; Brignell, C.; Harun, M.S.R.; Wehbe, K.; Cinque, G. Label-free characterization of biochemical changes within human cells under parasite attack using synchrotron based micro-FTIR. Anal. Methods 2019, 11, 2518–2530.. https://doi.org/10.1039/C8AY02777C. hBME cell were infected with T. gondii RH tachyzoite at MOI of 2 for 6, 24 and 48 hours.  Cells were lysed and total RNA were extracted using Qiagen RNeasy Plus Mini kit according manufacturer protocol. RNA quality were assesed with Agilent 2100 Bioanalyser  Messenger RNA (mRNA) libraries were prepared using Illumina's TruSeq Stranded mRNA HT Kit [RS-122-2103] according manufacturer protocol.