GSM6064667: DEN12WT, wildtype, DEN12, rep2; Mus musculus; RNA-Seq
Total RNA was harvested using TRIzol® Reagent(Invitrogen) according to the manufacturer's instructions. RNA quality was determined by 2100 Bioanalyser (Agilent) and quantified using the ND-2000 (NanoDrop Technologies). Only high-quality RNA sample (OD260/280 =1.8~2.2, OD260/230>=2.0, RIN>= 6.5, 28S:18S >=1.0, >10μg) was used to construct sequencing library. RNA-sequencing transcriptome library was prepared following TruSeqTM RNA sample preparation Kit from Illumina (San Diego, CA) using 1μg of total RNA.