GSM5129873: HCT116 cells, Input_1; Homo sapiens; OTHER
For ChIRP-DNA beads resuspend in 150ul DNA elution buffer (50 mM NaHCO 3, 1 % SDS), add RNase A and RNase H incubate at 7℃ for 30min with shaking, eluted chromatin was pK treated at 50℃ for 45 min with mixing. DNA was then extracted with equal volume of phenol:chloroform:isoamyl and precipitated with ethanol at -80℃ overnight. High-throughput sequencing libraries were constructed from ChIRPed DNA using ThruPLEX® DNA-seq kit (Rubicon Genomics) according to manufacturer's instructions and applied to illumina platform for 151 nt pair-end sequencing by ABLife. Inc. (Wuhan, China).