genome sequence of transgenic marmoset Tg1(755)
Genomic DNA quality was evaluated by 0.7% agarose gel electrophoresis. Genomic DNA for shotgun sequencing was sheared to an average fragment size of 600 bp (Covaris, MA, USA). Both ends of the fragmented DNA were blunt-ended and phosphorylated, and then the DNA was size sorted using Agencourt AMPure XP (Beckman Coulter, CA, USA). A TruSeq DNA PCR-Free LT Library Prep Kit (Illumina Inc. San Diego, CA, USA) was used to construct DNA libraries following the manufacturers protocol. The quality of constructed DNA sequence libraries was determined using Agilent TapeStation (Agilent tech., CA, USA). DNA libraries were sequenced on an Illumina NovaSeq 6000 with 150-bp paired-end sequencing (Illumina Inc. San Diego, CA, USA).