GSM5900239: DT40; Gallus gallus; OTHER
Okazaki fragments were isolated by sucrose gradient size-fractionation of genomic DNA followed by click-chemistry biotinylation and streptavidin capture of EdU-labelled DNA as described previously (PMID: 26751768). Okazaki fragments were treated with NaOH to remove 5' RNA, 5' phosphorylated with T4 polynucleotide kinase (PNK), hydridized and ligated to double-stranded adapters, followed by ampfication and bar-coding in PCR.