Illumina-seq of Zhuqinia jingwanae: adult whole body
After whole genome amplification samples were randomly interrupted by an ultrasonic breaker, and then the entire library preparation is completed through the steps of end repair, addition of A tail, addition of sequencing adapter, purification, PCR amplification, denaturation, and circularization. After the library is constructed, we used Qubit 3.0 to perform preliminary quantification of the library, and then we used Agilent 2100 to detect the inserted fragments of the library to ensure the quality of the library. After the library was qualified, we prepared DNB (DNA Nano Ball), then we loaded it on the sequencing chip of BGI high-throughput sequencer MGIseq2000 platform for sequencing.