GSM5239134: WT_minus_UV_rep2; Escherichia coli str. K-12 substr. MG1655; RNA-Seq
Samples were lysed with lysozyme and RNA extraction was performed using Mastpure DNA and RNA extraction kit (lucigen), treated with Turbo DNA Free and rRNA was removed using Ribominus (Thermo Fisher). Libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit.