GSM5324288: B2B07_4mo28_cumulus; Mus musculus; RNA-Seq
single oocyte or their surrownding cumulus cells pool were lysed using cell lysis buffer [10 U RNasein plus (Promega), 10% RealTime ready Cell Lysis Buffer (Roche), 0.3% NP40 (Thermo Fisher Scientific), and RNase-free water (TAKARA)] in 0.2 mL single tube. RamDA-seq sequencing libraries were constructed by using the advanced method of RamDA-seq (refer to the Methods section in "Single-cell full-length total RNA sequencing uncovers dynamics of non-polyadenylated RNAs, recursive splicing and enhancer RNAs" DOI: https://doi.org/10.1038/s41467-018-02866-0). However, to adapt to the manually picked single oocyte and their cumulus cells, the reaction volume from the cell lysis to the genomic DNA digestion step was modified. For details, refer to the protocol in "Aging-associated effects on transcriptome landscape and chromosome cohesion in single oocytes are influenced by life stage and calorie restriction".