GSM5628762: MCF7 cells, HSF1+/+_estrogen_4h_repeat 2; Homo sapiens; RNA-Seq
Total RNA was isolated from all MCF7 cell variants (untreated and treated with 10 nM E2 for 4 h or heat shocked for 1h at 43°C and recovered for 2 h) using the Direct-ZolTM RNA MiniPrep Kit (Zymo Research) and digested with DNase I (Worthington Biochemical Corporation). For each experimental point, RNAs from three biological replicates were first tested by RT-qPCR for the efficiency of treatments, then pooled and sequenced. For second CRISPR/Cas9 HSF1 knockout model (CRISPR/Cas9 HSF1+/+ mix and HSF1-/- mix samples) three independent biological repeats were done including RNA isolation and sequencing. RNA libraries were prepared for sequencing according to the standard Illumina protocols