D15_deltaIEC
Small intestines were excised and flushed thoroughly three times with PBS. They were turned inside out and cut into ~1 cm sections; then, sections were transferred into RPMI with 2mM EDTA and shaken for 20min at 37C. Supernatants were collected through a 100-mm cell strainer to obtain single-cell suspensions. Cells were collected as the intestinal epithelial cells (IEC) fraction, which contained both epithelial cells (~90%) and lymphocytes (IEL, ~10%). Total RNA was extracted from IECs using Trizol reagent (TaKaRa). RNA was purified according to the manufacturers instructions.