Loranthus seeds, cold treatment for 12 hours, replicate 2
Equal amount of total RNA (1 g) was used to construct the cDNA libraries for BGISEQ-500. In brief, mRNAs were enriched by magnetic oligo (dT) beads and fragmented into ~ 200 bp fragments, followed by the double strand cDNA library construction using random hexamer N6 primers. Next, the double strand cDNA libraries were end repaired by phosphate at the 5-end and sticky A to the 3-end. After the sequencing primers were ligated to each library and multiple libraries were pooled using the index technology, the pooled library was sequenced on the BGISEQ-500 RS platform with paired-end 150 (PE150) strategy at BGI-Shenzhen.