GSM5900137: H9_20Kcells_R1_Late S Fraction; Homo sapiens; OTHER
Genome-wide replication timing profiles were constructed as previously described (Hiratani et al. 2008; Ryba et al. 2011; Marchal et al., 2017). Briefly, cells were pulse labeled with BrdU and separated into early and late S-phase fractions by flow cytometry and processed by NGS. Sequencing libraries of BrdU-substituted DNA from early and late fractions were prepared by NEBNext Ultra DNA Library Prep Kit for Illumina (E7370) or by KAPA HyperPlus library preparation kit (Roche Cat. No.  07958978001).