mice fed a normal diet - ND rep 3
All mice were maintained at 22 Celsius, controlled moisture of 60 percent, and in 12h light/12h dark cycle with ad libitum access to food and water. After a three-day acclimation period of standard rodent food, the food was replaced by the experimental diets. Thirty-two male 7-8-week-old mice, (C57BL6J) were randomly divided into 4 groups (n=8 per group). Experimental groups were as follows: 1. normal-diet ND 2. HFD 3. HFD with Hanoch peanuts HFDh, 4. HFD with high oleic acid Hanoch-Oleic HFDo peanuts. The effects of each diet on the bacterial population in the gut microbiome were examined with the analysis of the prokaryotic 16S ribosomal RNA gene 16S rRNA, using a two-step PCR-based method for preparing samples for sequencing the variable V3 and V4 regions of the 16S rRNA gene