Fecal sample DonorRTX1_3
Libraries were prepared according to the protocol of the Earth Microbiome Project (https://earthmicrobiome.org/protocols-and-standards/16s/). Amplicon libraries were washed using Beckman Coulter AMPure XP magnetic beads. Library quality and size verification was performed using a PerkinElmer LabChip GXII instrument with DNA 1K Reagent Kit (CLS760673). Library concentrations were quantified using Quant-iT dsDNA High Sensitivity Assay Kit using a Promega GloMax plate reader on a microplate (655087). Library molarity was calculated based on library peak size and concentration. Libraries were normalized to 2nM using the PerkinElmer Zephyr G3 NGS Workstation (133750) and pooled together using the same volume across all normalized libraries into a 1.5 ml Eppendorf DNA tube (022431021).