DNA-Seq of Rubus setchuenensis:leaf
Fresh leaves of Rubus setchuenensis Bureau & Franch. were collected from Tianquan District in Sichuan Province (3001.180N, 10247.574E), then dried and kept in silica gel for DNA extraction. Total genomic DNA was extracted with the DNeasy plant Kit of Aidlab and sequenced by the Illumina nova-seq 6000 (Illumina Inc., San Diego, CA). The length of raw sequence reads is 11,269,294,800bp. De novo assembling was conducted using GetOrganelle (Jin et al. 2018). Plann v1.1 (Huang and Cronk 2015) and Geneious v11.0.3 (Kearse et al. 2012) were used to annotate the chloroplast genome and correct the annotation, with the complete plastome sequence of R. coreanus (Genbank Accession number NC_042715) as reference.