GSM5239403: Testis_RNA_Th2; Mus musculus; RNA-Seq
Testes stored at -80°C from 35-day-old mice from the control group and from the 6 mg/kg/day treatment group were used for RNA extraction. Extraction was performed using RNeasy Plus Mini Kit (QIAGEN®). Approximately 30 mg of each testis was used. A strand-specific library preparation protocol (NEBNext Ultra II Directional RNA Library) for RNA sequencing was performed in the lab according the protocol provided by manufacture by using 1µg of total RNA.