GSM5566264: Nuclear matrix poly(A)- RNA-seq rep1; Homo sapiens; RNA-Seq
RNA from the lysates of different cellular fractionations or total RNA was extracted by TRIZOL reagent. Libraries of the RNA-seq were prepared according to NEB's protocol of the NEBNext® Ultra™ II directional RNA library prep kits. Libraries of the SLAM-seq were prepared according to Lexogen's instructions of QuantSeq 3'mRNA-seq library prep kit REV for Illumina. Nanopore libraries were prepared and sequenced according to Oxford's instructions for direct RNA sequencing.