Whole Bone Marrow in vivo tamoxifen treated  hScl-CreER Srsf2+/+ Tet2+/+ 681 55 weeks post tamoxifen
Exome sequencing was performed on genomic DNA from whole BM of 11 independent animals from 50 to 60 weeks post–tamoxifen treatment or when moribund. DNA was isolated using Gentra Puregene kit (Qiagen) as described by the manufacturer. Exome capture was performed by GENEWIZ with the Agilent SureSelect Mouse Exon system and sequenced on the Illumina platform generating 150bp PE reads (GENEWIZ Ltd, Hong Kong)