Illumina WGS of Vibrio alginolyticus isolate RM-32-1 from fresh (not frozen) retail oysters, 2015
NexteraXT paired-end (2x300, v3 chemistry) on MiSeq. DNA Prep: Frozen stock struck onto tryptic soy agar plates with 2% NaCl (TSA-2N) and incubated at 35 C overnight. An isolated colony was used to inoculate 5 mL tryptic soy broth with 2% NaCl (TSB-2N) at 35 C for 24 h, and DNA was extracted using the Maxwell 16 SEV Cell DNA purification kit.