16S rRNA of Homo sapiens: adult man stool at baseline
The total microbial DNA from the stools of each participant, collected at baseline, at the end of the intervention or one month after the end of the intervention, was extracted using the PowerSoil DNA Isolation Kit (Qiagen, Carlsbad, CA) following the manufacturers instructions.  The V4 hypervariable region of the 16S rRNA gene of each sample was amplified using primers F515 and R806. Libraries were prepared following the dual-index sequencing strategy (Kozich et al. 2013, Appl. Environ. Microbiol. 79(17):5112). Sequencing was performed in an Illumina MiSeq sequencing platform with V2 chemistry.