GSM5628757: dSVZ_P12_2; Mus musculus; RNA-Seq
The dorsal subventricular zone (dSVZ) from P12 mice (2 males) of 2 independent experiments were dissected. During all stages of the dissociation protocol, the tissue was kept in aCSF solution containing 125 mM NaCl, 2.5 mM KCl, 1.25 mM NaH2PO4, 26 mM NaHCO3, 17 mM glucose, 1.25 mM CaCl2 and 1 MgCl2. The small dissected tissues were incubated in Papain during 20 minutes at 37°C. Following enzymatic digestion, tissues were triturated and resuspended on ice in Leibovit'z L-15 medium supplemented with 0.1% of bovine serum albumin. The cell suspension was then filtered through a 70-μm cell strainer to remove aggregated cells. mRNA libraries were prepared according to 10x Genomics 3' version 3 chemistry & Illumina protocols