GSM6214344: Figue 4E control mice 2; Mus musculus; OTHER
The genomic DNA of liver samples were obtained by treatment with chorion villi buffer followed by isopropanol precipitation NGS was performed by Eurofins using the INVIEW CRISPR Check - 2nd PCR service with MiSeq Sequencing, 60k read pairs (2x300 bp). Briefly, genomic sites of interest were PCR-amplified using Phusion Hot Start II PCR Master Mix with primers containing forward and reverse adapters. The PCR products were agarose gel-purified and submitted to Eurofins for library construction and sequencing.