GSM5708444: ESC_H33WT_H3K4me3; Mus musculus; ChIP-Seq
Native ChIP was performed as described (Goldberg et al. 2010). Briefly, cell were harvested treated with Mnase to produce fragments of 200-700 bp. Chromatin was isolated and used for ChIP according to standard procedures. Libraries were constructed according to the Illumina Tru-seq protocol.