GSM5804882: CD8+ T cells_Spleen rep2; Mus musculus; RNA-Seq
Each data point reflects pooled cells from two mice.  Spleens were bisected, ½ spl from each mouse mechanically disaggregated between frosted glass slides then pooled.  Gt from same two mice were processed together using a modification of a collagenase/hyaluronidase cocktail and mechanical disaggregation protocol. CD3+CD4+CD8-Ig-, CD3+CD8+CD4-Ig- cells were sorted from each tissue. RNA libraries were prepared for sequencing with TruSeq Stranded mRNA Kit (Illumina)