16S-DNA-Seq of soil:10
Undried bulk soil samples were collected from each pot and genomic DNA extracted in triplets using DNeasy PowerSoil Kit (Qiagen, Hilden, Germany) following the manufacturers instructions. 16SrRNA V3-V4 hypervariable region was PCR amplified using Illumina adaptor ligated universal primers 515F-Y(5-GTGYCAGCMGCCGCGGTAA-3) and 806RB(5-GGACTACNVGGGTWTCTAAT-3) from each triplet. The thermocycler condition was maintained at 94oC for 3 min, followed by 25 cycles of 94oC for 30 s, 55oC for 30 s, 72oC for 30 s and a final extension of 72oC for 3 min.