GSM5900043: naïve XH001 passage 0 time 6 rep 2; Schaalia odontolytica; RNA-Seq
Cell pellets were resuspended in cold PBS buffer and beaten with glass beads 3 times for 30 seconds with 30 second breaks at 6 m/s speed. Total RNA was isolated using the High Pure RNA isolation kit. Isolated RNA was cleaned and concentrated with Zymo Research RNA clean & concentrator kit following kit protocol. DNA was removed using the Ambion TURBO DNA-free kit. rRNA was depleted with Ribo-Zero. RNA sequencing library preparation used NEBNext Ultra RNA Library Prep Kit