GSM5322531: dQ_snf5_Dex_1; Saccharomyces cerevisiae; RNA-Seq
Total RNA was extracted from WT, ΔQ-snf5 and HtoA-snf5 strains during exponentially growth (+Glu) and after 4 hours of glucose starvation using a Roche Hi-Pure RNA extraction kit. Next, Poly-A selection was performed using Dynabeads and libraries were performed following manufactures indications. RNA libraries were prepared for sequencing using standard Illumina protocols