GSM5239266: STAT5B pull down SUPT1 with overexpression of STAT5B wild type (WT), untreated; Homo sapiens; ChIP-Seq
20x10^6 cells were fixed and chromatin-immunoprecipitation (ChIP) was performed according to the manufacturer's instructions (Simple ChIP Enzymatic Chromatin IP Kit, #9003, CST). Water bath sonification was performed for seven cycles (30 seconds on 30 seconds off), and DNA fragment size was validated by electrophoresis. DNA library preparation was performed using the NEBNext Ultra II DNA Library Prep kit (New England Biolabs #E7103) with an additional 0,52X beads purification step.