GSM5130057: 8_high proviral copies; Capra hircus; RNA-Seq
The total RNA was isolated from whole blood of  goats selected based on of provirus copy number,  and which were the most homogeneous within the groups. Isolation was performed using MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit (Thermo Fisher Scientific), according to the protocol. The possible RNA contamination with DNA was removed using TURBO DNase™ (Thermo Fisher Scientific). The quality and quantity of obtained RNA were check using Nanodrop 2000 Spectrophotometer (Thermo Scientific) and TapeStation 2200 System (Agilent) using Agilent RNA ScreenTape (Agilent). The samples with RIN (RNA integrity number) value above 8 were used to further analysis. The cDNA libraries were prepared from 300ng of total RNA by using TruSeq RNA Kit v2 kit (Cat#FC-122-1001) according to protocol.