RNA Seq Of Mesocricetus auratus
Total RNA was subjected to two rounds of poly(A) selection using oligo-d(T)25 magnetic beads (New England Biolabs). Illumina compatible RNAseq library was prepared using NEB next ultra RNAseq library preparation kit. The cDNA libraries were purified using AmpureXP beads and quantified on an Agilent TapeStation and Qubit 4 Fluorometer (Thermo Fisher Scientific). An equimolar amounts of barcoded libraries were pooled and sequenced on the Illumina NovaSeq  platform (Illumina, San Diego, CA)