scRNA-deq of Mus musculus: adult liver
To generate single-cell suspensions, liver isolated from adult Fah-injury mice was perfused with enzymatic solution, then filtered through a 70m cell strainer. tdT-positive BECs and hepatocytes are sorted by FASC. Library was prepared with Chromium Single cell 3' Reagent v3.1 Kits according to the manufacturer's protocol.