GSM5015669: primary human islets-antiFlag CUT&RUN; Homo sapiens; OTHER
Lysates were clarified from Protein A-micrococcal nuclease digested DNA. DNA fragments were released for 20 min at 37°C and purified using phenol/chloroform/isoamyl alcohol followed by chloroform extraction and precipitated with glycogen and ethanol. SIX2-Flag-DNA complexes were isolated with anti-Flag antibody. NEBnext Ultra II library kit. CUT&RUN (similar to ChIP-seq) and sequenced as 2 × 75 on HiSeq4000.