GSM5741296: Aged_PGE2_1; Mus musculus; ATAC-seq
Cells were pelleted and permeabilized in hypotonic buffer with 0.1% IGEPAL CA-630. Nuclei were treated with transposase from Illumina (Cat FC-121-1030). Fragments were amplified with NEBNext PCR master mix and Nextera indices for 8-12 cycles.