shotgun metagenomic seq of Homo sapiens: RCDI PostFMT K 4 month stool
Approximately 1.8 mls frozen whole stool samples from donors and patients were sent to Admera Health, LLC (South Plainfield, NJ) for DNA extraction and library preparation. In brief, the gDNA was extracted from whole stool using the NucleoSpin Soil kit (Macherey-Nagel) and the concentration of gDNA was assessed by the Qubit dsDNA High Sensitivity assay kit (Thermo Fisher). Libraries were prepared with the Nextera XT DNA Library Preparation Kit (Illumina) following the manufacturers protocol. The concentration of final libraries was measured by qPCR and equal amounts of DNA libraries were pooled, denatured with 0.2N NaOH, and then run on the NovaSeq S4, generating 20-100M 150-nt paired-end reads per sample.