GSM5530902: GEX_L001; Homo sapiens; OTHER
Stained cells were then encapsulated for single-cell reverse transcription using the 10X Chromium platform (5' v1.0) cDNA amplification was performed in the presence of 2pM of an antibody oligo specific primer to increase yield of antibody derived tags (ADTs) and 3pM of specific primer to increase yield of HTOs. The amplified cDNA was then separated by SPRI size selection into cDNA fractions containing mRNA derived cDNA (>300bp) and ADT-derived cDNAs (<180bp), which were further purified by additional rounds of SPRI selection. Independent sequencing libraries were generated from the mRNA and ADT cDNA fractions, which were quantified, pooled and sequenced together OTHER: CITE-seq