GSM5484642: ovarian granulosa cells from older women OZJM IP; Homo sapiens; OTHER
Total RNA of granulosa cells were extracted using RNA-easy Isolation Reagent according to the manufacturer's instructions. The quality of the raw reads was determined by FastQC. Trimming was performed for known Illumina TruSeq adapter sequences, poor reads, and ribosomal RNA reads. Then, clean reads were aligned to the human reference genome with the use of Hisat2 software (v2.0.4). immunoprecipitation