Metabarcondig of endolithic communities: bacteria
The 16S V4 region was amplified using the new developed barcoded F515/R806 primer set (Caporaso et 2012 al., ISME J, 6, 16211624). The PCR for the 16S V4 region followed a protocol of an initial denaturation at 94 C for 3 min, 35 cycles of denaturation at 94 C for 45 s, annealing at 50 C for 1 min, extension at 72 C for 90 s, followed by a final extension at 72 C for 10 min. Amplicons, quantified by a Qubit dsDNA HS Assay Kit (Life Technologies, Carlsbad, CA, USA), were pooled and then purified with Qiagen PCR CleanUp kit (Macherey-Nagel, Hoerdt, France). Paired-end sequencing (2  300 bp) was carried out on an Illumina MiSeq platform.