GSM5491585: WT_CDX2_ChIP-seq; Homo sapiens; ChIP-Seq
Tagmentation was performed for 1 hr in Dig-300 buffer and then fragmentated with 300µL tagmentation buffer incubated for 1 hr at TM. Extracted nucleic acid with phenolic chloroform and dissolved in 1xTE solution. The nucleic acid samples suspension was amplificated 15-20 cycles with 50µL amplification mix (TAE, TAB and P5, P7 primer) (TD901, Vazyme), and purified using VAHTS DNA Clean Beads (N411, Vazyme)