GSM5209374: ChIP-seq_WT_H3K27me3; Mus musculus; ChIP-Seq
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP's DNA was subjected to end-repair and then was 3'adenylated. Adaptors were ligated to the ends of these 3'adenylated fragments. Next, polymerase chain reaction amplification was to amplify those fragments with adaptors from previous step.