GSM5016969: Mock Infection Native ChIP- Total H3 IP; Saccharomyces cerevisiae; Homo sapiens; ChIP-Seq
Human chromatin was purified by cell lysis, MNase digest, and ultrasonic liquid sonication in a custom ChIP buffer for protein VII purification. Yeast native chromatin was purified from cells and ChIP performed as in PMID: 23468649. Libraries were prepared from the ChIP samples as well as a 10% input, following manufacturer recommendations, with KAPA Biosystems Unique Dual-Indexed Adapter Kit (#KK8727), Hyper Prep Kit (#KK8504), and Beckman Coulter AMPure XP Beads (#A63880) for library cleanup and size selection. Library concentrations were measured with Invitrogen Qubit dsDNA HS Assay Kit (#Q32851), then analyzed with Agilent High Sensitivity D5000 ScreenTape System, and pooled. Libraries were sequenced with 50-bp paired-end reads using the Illumina NovaSeq 6000 SP Flow Cell on an Illumina HiSeq 2500 sequencer at the Fred Hutch Genomics Core Facility.