GSM5741637: KNA cells - diabetic; Homo sapiens; RNA-Seq
The cells were then detached, washed in PBS, and resuspended in 200µL of TRIzol® Reagent. Pure RNA was collected using Zymogen Direct-zol RNA MiniPrep kit (Zymo Research) and the 260/280 ratio was determined to be ≥2 for each sample. RNA quality was further assessed using a Bioanalyzer (Agilent) and all samples had a RIN >7. Illumina paired-end 75 base pair sequencing was used with 25 million reads per sample.