GSM5901508: AAV_CNTF_optic_nerve_crush_rep3; Mus musculus; RNA-Seq
Libraries were prepared with the Nugen Universal RNASeq with AnyDeplete Mouse kit using 20ng RNA input and sequenced on Illumina Novaseq 6000. Total RNA was extracted from the optic nerve using RNeasy Micro Kit (Qiagen 74004) and treated with DNAse I to remove contaminating genomic DNA. Total RNA-seq (ribo-depleted) libraries were produced using Truseq Stranded Total RNA library prep kit (Illumina, #20020596) with 500 ng of DNase-treated Input RNA.