GSM6183473: murine bone marrow sample (pool: 3 mice)_HTO; Mus musculus; OTHER
The primary mouse cells were labeled with hashtag TotalSeq-A0304 anti-mouse Hashtag 4 Antibody from Biolgend (#155807), secondary bone marrow tranplanted with Lin- LSCs was labeled with TotalSeq-A0305 anti-mouse Hashtag 5 Antibody from Biolgend (#155809), and secondary bone marrow transplanted with Lym+ LSCs was labeled with TotalSeq-A0306 anti-mouse Hashtag 6 Antibody from Biolgend (#155811) Library preparation for scRNA-seq analysis was performed according to the Chromium NextGEM Single Cell 3ʹ Reagent Kits v3.1 User Guide (Manual Part Number CG000204 Rev B; 10x Genomics). An excess of cells was loaded to the 10x controller in order to reach a target number of 18000 cells per 'master sample'. Fragment length distribution of generated libraries was monitored using 'Bioanalyzer High Sensitivity DNA Assay' (5067-4626; Agilent Technologies). Quantification of libraries was performed by use of the 'Qubit® dsDNA HS Assay Kit' (Q32854; ThermoFisher Scientific).