GSM5988618: PDR Patient 1 [PDR_1]; Homo sapiens; ncRNA-Seq
Total RNA was extracted from vitreous humour using the TRIzol reagent (Invitrogen/Life Technologies) following the manufacturer's protocol. RNA concentration was quantitated using the NanoDrop ND-100 Spectrophotometer (NanoDrop Technologies, Wilmington, DE).Total RNA was pretreated to remove  some modification which interfere with small RNA-seq library construction(e.g. m1A). 1) 3'-adaptor ligation;2) 5'-adaptor ligation;3) cDNA synthesis;4) PCR amplification;5) size selection of 134-160 bp PCR amplified fragments (corresponding to 14-40 nt small RNAs).