WGS of bacteria from ready-to-eat foods in Nigeria
Two isolates from RTE foods from Nigeria-based AMR gene profiles were selected for WGS. Whole-genome libraries were conducted by following the protocol described in the Illumina DNA Prep Tagmentation sample preparation kit (Illumina, San Diego, CA). This protocol entails a tagmentation step of genomic DNA, followed by a post-tagmentation purification step, and then amplification of tagmentation DNA. Lastly, the libraries are cleaned and pooled. Sequencing then takes place by loading the 4 nM pooled library onto the Illumina MiSeq platform using the MiSeq Reagent Kit V3.