GSM5208244: ATAC-seq 48hours BM/Ikzf1/4 rep1; Mus musculus; ATAC-seq
48 hours after doxycycline addition, 50000 MEF nuclei were isolated as previously reported (Buenrostro et al., 2015), with modifications described in (Mayran et al., 2018). Isolated nuclei underwent transposase reaction: 2.5μl of 10x TD buffer, 10μl of water, and 12.5μl of enzyme (Illumina Nextera kit; FC-121–1031). DNA was purified with GeneRead Purification columns (Qiagen), enriched by PCR, and purified again with GeneRead Purification columns before being sequenced.