GSM5135082: MCF7 E2 siMETTL314 YTHDC1 ChIP-Seq rep2; Homo sapiens; ChIP-Seq
Lysates were clarified from sonicated nuclei and chromatin were isolated with specific antibody. DNA libraries were prepared using NEB Next Ultra II DNA library prep kit for illumina according to manufactorer's instructions RNA was extracted using TRIzol (Life Technologies), 4sU-labeled RNA was exctracted according to Duffy et al., Mol Cell, 2015.  M6A methylated RNA was immunoprecipitated by anti-m6A antibodies (202003, SYSY). RNA libraries were prepared using NEB Next Ultra II RNA library prep kit for illumina according to manufactorer's instructions