GSM4908644: Non-myelinating Corneal Schwann Cells scRNA-seq; Oryctolagus cuniculus; RNA-Seq
Corneal buttons from central aspect of the tissue from young adult male rabbits (after mechanical removal of endothelial cell layer and trypsin digeston for partial removal of epithelium) were dissociated into single cell suspension, filtered, centrifuged , and resuspended in ice-cold 1X PBS with 0.05% BSA and immediately processed for scRNA-seq analysis. scRNA libraries were prepared for sequencing using Chromium system (10x Genomics; (Zheng et al., 2017)) for droplet-based single cell library generation with v3 Chromium chemistry and processed for cDNA library construction and subsequent scRNA-seq analysis (Rheaume et al., 2018)