CLR-seq of Emiliania huxleyi AWI1516  calcified cells 6h in light phase - Std DNA extraction protocol - SMRT cell 2
For the extraction of high molecular weight DNA, cells were disrupted using the Tissuelyser II (Qiagen) and Lysing Matrix E (MP Biomedicals) and DNA was purified using the NucleoSpin Food kit (Macherey and Nagel). DNA quality was assessed using the Agilent FEMTOpulse system and DNA quantity was measured using Thermo Scientific Qubit system. PacBio libraries were prepared from unfragmented DNA with the SMRTbell Template Prep Kit 1.0 (Pacific Biosciences) followed by size-selection for enrichment of fragments 10 kb (Blue Pippin, SAGE Science). After purification with magnetic beads, the library was sequenced on the Sequel with Sequel DNA polymerase and binding kit version 3.0 and sequencing chemistry version 3.0 for 1,200 minutes.