GSM5725944: CRE4_S49; Mus musculus; RNA-Seq
Teeth were dissected and pulp was removed from the mineralized structure and immersed in Lysis Buffer (Quiagen #79216).  Samples were pestled to break up tissue and RNA was isolated with RNA Sample Prep Tool (Zymo Research #R2072). RNA Integrity Number (RIN) and RNA quantification for total RNA were determined by Agilent BioAnalyzer RNA 6000 Pico Kit (Cat. # 5067-1513) and Invitrogen Qubit RNA HS Assay kit ( Cat. # Q32852), respectively. Samples with RIN > 7 were used for global preamplification for the 1-ng low-input mRNA-seq using the Clontech SMAR-seq HT kit (Cat. # 634437). The resultant cDNA samples were used for library generation using the Illumina Nextera XT DNA Library Prep Kit (Cat. # 15031942) and sequenced on Illumina NovaSeq SP flowcell for 12-15 million PF clusters per sample. 1ng-Low Input mRNA-Seq