GSM5133938: MGH51_081413; Homo sapiens; RNA-Seq
RNA was purified from patients' frozen or formalin-fixed tumor specimens using the TRIzol protocol.  Illumina TruSeq RNA Sample PRE Immune Checkpoint Blockade Therapyp Kit (Cat#FC-122-1001) was used with 250 ng of total RNA for the construction of sequencing libraries. RNA libraries were PRE Immune Checkpoint Blockade Therapypared for sequencing using standard Illumina protocols