GSM4793333: prp39a_2; Arabidopsis thaliana; RNA-Seq
Total RNA was extracted using RNeasy plant mini kit (Qiagen) from whole 14-day-old Col-0, prp39a-3 (SAIL_1249A03), smd1b-3 (Elvirat-Matelot et al., 2016) and Col-0 plants grown on 1/2MS medium. Three independent biological replicates were produced per genotype. For each biological repetition and each point. After RNA extraction, polyA RNAs were purified using Dynabeads mRNA direct micro kit (Ambion). Libraries were constructed using the Tru-Seq Stranded mRNA Sample Prep kit (Illumina®). RNA libraries were prepared for sequencing using standard Illumina protocols