5 instar-O colony-16:8-R3
For transcriptome sequencing, total RNA from O. furnacalis fifth instar larvae of O colony reared in long daylenth (L:D=16:8) were collected and extracted using TRIzol reagent (Invitrogen, CA, USA). Sequencing libraries were generated constructed using an the TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA, USA), and sequencing was also performed on the Illumina NovaSeq 6000 platform.