GSM5801214: CS2_S653; Homo sapiens; RNA-Seq
Blood KIR+ CD8+ T cells (Live CD3+CD56–CD8+TCRαβ+KIR+ cells) were sorted into 96-well plates and cDNA synthesis was performed using the Smart-seq2 protocol Illumina sequencing libraries were prepared using a Mosquito HTS liquid handler (TTP Labtech). In brief, tagmentation was carried out on 0.4 μl double-stranded cDNA using the Nextera XT DNA Library Preparation Kit (Illumina, FC-131-1096). Each well was mixed with 0.8 μl Nextera tagmentation DNA buffer (Illumina) and 0.4 μl Amplicon Tagment Mix (Illumina), then incubated at 55°