GSM4794562: virus-negative control [P010]; Homo sapiens; RNA-Seq
Total RNA was extracted from blood samples using the Tempus Spin RNA Isolation Kit and processed for globin reduction using GLOBINclear Human Kit (Thermo Fisher Scientific). RNAseq was performed using cDNA libraries generated from 200 ng of globin reduced total RNA from each sample. Libraries were constructed with the NexteraXT library kit (Illumina, San Diego, CA) and sequenced (single end reads) on the Illumina HiSeq2500 (Illumina, San Diego, CA) to generate 20 million reads/sample.