GSM4907821: Bint_InWAX rep2; Bacteroides intestinalis; RNA-Seq
The cells were rapidly mixed with RNAprotect bacterial reagent and collected by centrifugation, as described by the manufacturer and used for subsequent RNA extraction. RNA was extracted using the RNeasy mini kit (Qiagen, Valencia, CA) according to the manufacturer's instructions. Contaminating DNA was removed in-column using NEB DNase I (Ipswich, MA), and the quality of the RNA was assessed by using a Bioanalyzer 2100 with a RNA 6000 Nano Assay Reagent kit from Agilent (Santa Clara, CA). RNA libraries were prepared for sequencing using standard Illumina protocols