GSM5948918: Batch2_nam8D_48h_28H5_2; Saccharomyces cerevisiae; RNA-Seq
Total RNA was isolated from cells grown in log or stationary phases using hot acid phenol extraction and DNase treatment (Qiagen) was performed. RNAseq librairies were generated from 1ug DNA-free total RNA using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (E7760S) and following the Protocol for use with NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490)