GSM5808403: RNAseq DKO rep1; Homo sapiens; RNA-Seq
Total RNA were isolated from the cells using RNeasy Mini Kit (Qiagen) together with performing on-column DNase digestion (Qiagen). RNA concentrations were determined by Nanodrop ND1000 (Thermo Scientific). The quality of the RNA extracted was analyzed using Agilent RNA 6000 Nano Kit (Agilent), and quantitated using Nanodrop ND1000 (Thermo Scientific). Library construction was performed using TruSeq Stranded Total RNA LT (w/ Ribo-Zero Gold) Set A (Illumina) as per protocol.