DNA (WGS); Ovis aries; Sudan; Hammari
Next Generation sequencing libraries are prepared using Illumina SeqLab specific TruSeq Nano High Throughput library preparation kits in conjunction with the Hamilton MicroLab STAR and Clarity LIMS X Edition. The gDNA samples are normalised to the concentration and volume required for the Illumina TruSeq Nano library preparation kits, then sheared to a 450bp mean insert size using a Covaris LE220 focused-ultrasonicator. The inserts are ligated with blunt ended, Atailed, size selected, TruSeq adapters and enriched using 8 cycles of PCR amplification.