GSM5210193: US1_6hr_1; Drosophila melanogaster; Homo sapiens; Human alphaherpesvirus 1; OTHER
Nuclei were extracted as previously described (Birkenheuer and Baines 2020, Journal of Virology) nascent RNA was labelled with biotinylated nuclotides during a nuclear run-on.  The biotinylated transcripts were isolated and used to prepare sequencing libraries from the reverse transcribed products. Libraries were constructed as previously described (Mahat and others, 2016,  Base-Pair Resolution Genome-Wide Mapping Of Active RNA polymerases using Precision Nuclear Run-On (PRO-seq), Nature Protocols). Libraries were created using Illumina Index primers for sequencing on the NextSeq550 75 base pair single end sequencing.