RNA-seq of Nasonia vitripennis, adult female
For RNA extraction, five individual wasps were pooled and crushed to fine powder under liquid nitrogen. Total RNA was extracted using AllPrep DNA/RNA Mini Kit (Qiagen, Hilden, Germany) according to manufacturers protocol. We further purified our samples using DNase I (RNase-free, HC, Life Technologies, Waltham, U.S.A.). We quantified RNA using RNA HS Assay Kit (Life Technologies, Waltham, U.S.A.) on the Qubit 2.0 Fluorometer (Life Technologies, Waltham, U.S.A.) and qualified using a NanoDrop 2000/2000c Spectrophotometer (Life Technologies, Waltham, U.S.A.).