GSM4793183: RNA-Seq TotalRNA_Control_rep2; Homo sapiens; RNA-Seq
Cells were lysed with 400ul lysis buffer (20mM Tris-Cl pH 7.4, 150mM NaCl, 5mM MgCl2, 1mM DTT and 100ug/ml CHX, 25U/ml Turbo DNase, 1% Triton X100), 50ul lysate taken for total RNA and the rest digested with RNase I and ran on a sucrose cushion to produce the RPF sample All samples were rRNA depleted using Illumina RiboZero Gold; libraries for Ribo-Seq were prepared with Nextflex small RNA-seq; libraries for RNA-Seq were prepared with Nextflex rapid directional qRNA-seq