GSM5208240: scRNA-seq - Ikzf1/4; Mus musculus; RNA-Seq
Electroporated retina pieces were dissociated in papain (Worthington). YFP+ cells from mCherry+ regions of 7 retinas (n=7) were pooled. Fluorescent cells were sorted with a FACSAria III Cell Sorter (BD Biosciences). Viable YFP+ cells (single cells) were collected in PBS with 0.15% BSA (Millipore). Cells were then spun at 300g for 10 minutes at 4°C and re-suspended in 0.15% BSA/PBS. Cells were loaded on a 10xGenomics Single Cell 3' chip and processed according to manufacturer V3 pipeline 3.1.0 kit.