GSM5016437: s_testis_Mov10l_KO_13dpp_F64-M3_r2.PE; Mesocricetus auratus; RNA-Seq
Total RNA was extracted from adult, 21dpp, 13dpp and 9dpp hamster testes using Sigma-Aldrich miR Premier micro RNA isolation kit according to the manufacturer´s protocol. Ribosomal RNA (rRNA) was depleted from RNA used for transcriptome analysis using Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat) (Epicentre) according to the manufacturer´s protocol. rRNA depletion was confirmed by 2100 Bioanalyzer (Agilent Technologies). RNA-seq libraries were generated using NEBNext Ultra II directional RNA library Prep kit for Illumina (BioLabs, #E7765S) according to the manufacturer´s protocol. RNA-seq libraries from adult, 21dpp and 13dpp testes were sequenced by 150 nt pair-end reading and RNA-seq libraries from 9dpp testes were sequenced by 75 nt single-end reading using the Illumina NextSeq500/550 platform.