GSM5017660: Macrophages-apoptotic cells_rep4; Mus musculus; RNA-Seq
RNA of macrophages was isolated using RNeasy Micro Kit (Qiagen, Hilden, Germany) according to the manufacturer´s instructions. Sequencing libraries were prepared using QuantSeq 3' mRNA-Seq Library Prep Kit with the UMI Second Strand Synthesis Module for QuantSeq (Lexogen, Vienna, Austria). RNA sequencing was performed using a High Output Kit v2 on a NextSeq 500 sequencer (Illumina, San Diego, USA). RNA libraries were prepared for sequencing using standard Illumina protocols