GSM5596576: HuH-7-T3-NI-2; Homo sapiens; RNA-Seq
RNA was extracted using the RNeasy Mini Kit (Qiagen) and genomic DNA was removed using TURBO DNA-freeTM kit (Ambion), according to the manufacturer's instructions. The integrity, purity and concentration of RNA samples was assessed on an RNA 6000 pico chip using the Agilent 2100 electrophoresis Bioanalyzer.  RNA was quality checked an then store at -80°C. RNA-seq libraries of infected and non-infected cell RNA were assembled using 500-1300 ng total RNA using the TruSeq® mRNA Stranded Library Prep kit (Illumina) which includes polyA-selection. The RNA-seq libraries were monitored for quality on the Bioanalyzer 2100 using an Agilent High Sensitivity DNA Kit. Libraries were pooled in equimolar proportions