GSM5988613: E13.5 Osr1 GCE/+ scRNAseq; Mus musculus; RNA-Seq
Axillary, cervical and inguinal regions of a E13.5 Osr1 GCE/+  embryo were extracted, minced and digested using a collagenase A solution in growth medium. Cell suspensions were stained with propidium iodine prior FACS. GFP-expressing live cells were isolated using a FACS Aria II device and collected directly into Dulbecco´s Modified Eagle Medium containing 4.5 g/l glucose and 10% fetal calf serum. 10,000 collected cells were run using the 10x Chromium (10x Genomics) system with the Chromium Single Cell 3' chemistry V3 Kit according to the manufacturer's recommendations. Libraries were sequenced on an Illumina HiSeq 4000 device using the following read specifications: 8‐bp index, 28‐bp read 1 (16‐bp barcode and 12‐bp UMI), and 98‐bp strand‐specific read 2.