GSM5134540: hDRG4; Homo sapiens; RNA-Seq
Dorsal root ganglia were harvested and incubated in RNAlater (ThermoFisher) at 4oC overnight.  Tissues were then transferred and stored at -80oC until use.  Tissues were homogenized using a glass dounce homogenizer on ice.  Neuronal nuclei were isolated using rabbit anti-NeuN antibody (Abcam) followed by anti-rabbit IgG microbeads (Miltenyi Biotec) and LS column (Miltenyi Biotec). Libraries construction were performed using 10x Genomics according to the manufacturer's instructions using v3 chemistry kits.