GSM5741647: Aged Sham GFP+, CD45-, CD31-, EpCAM- fibroblasts 4; Mus musculus; RNA-Seq
RNA quality was determined using the Fragment Analyzer from AATI (AATI, Ankeny, IA). RNA samples that have RQN values ≥6 were approved for library prep and sequencing. RNA libraries were prepared using 200 ng of good quality total RNA according to the manufacturer's instructions for the TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego, CA), employing poly-A mRNA enrichment using oligo dT magnetic beads. The final adapter-modified cDNA fragments were enriched by 12 cycles of PCR using Illumina TruSeq PCR primers. The concentration and size distribution of the completed libraries were determined using a Fragment Analyzer (AATI, Ankeny, IA) and Qubit fluorometry (Invitrogen, Carlsbad, CA).  RNA libraries were prepared for sequencing using standard Illumina protocols. Libraries were sequenced following Illumina's standard protocol.