GSM5949191: D283_SMARCA4_shRNA1; Homo sapiens; RNA-Seq
Total RNA was extracted using Qiagen RNAeasy mini kit as per manufacturer's protocol. Librarieswere prepared from Poly-A enriched fraction of total RNA using the Illumina TruSeq RNA library preparation kit version 2.0 The libraries were sequenced in a 100 nucleotide single-end read run on the Illumina Hiseq 2500 platform