GSM5241682: rad53-1 mrc1-aid +IAA BrdU IP rep1; Saccharomyces cerevisiae; OTHER
eSPAN in yeast was performed exactly as previously described (Yu et al., 2014). For eSPAN libraries, the supernatants were purified with ChIP DNA concentrator columns and library PCR was performed using standard Illumina Nextera Dual Indexing primers. Samples were pooled and sequenced using paired-end sequencing on Illumina platforms at Columbia University Genome Center.