RNA-Seq of a B. melanopygus: worker 0-hr callow stage
Dissected tergites were homogenized in their tubes for 35 sec on low in Trizol buffer using the Omnibead ruptor, with 4 metal beads, followed by extraction using standard protocols in the Direct-zol RNA Miniprep Plus kit, including the DNase I removal step. Sufficient RNA quality and quantity were confirmed using an Agilent Bioanalyzer, followed by preparation for Illumina sequencing using a TruSeq Stranded mRNA Kit (Illumina). Multiplexed libraries were sequenced at 75 bp paired-end using the Illumina NextSeq 550 at the Penn State Genomics Core Facility (University Park, PA)