Metagenomic nanopore sequencing of Ferrovum dominated stalactite biofilm from acid mine drainage
Total genomic DNA was isolated from stalactite biofilm using DNeasy PowerLyzer PowerSoil kit (Quiagen, Germany). The WGA (Whole Genome Amplification) method was used to amplify the DNA using REPLI-g Mini Kit (Qiagen) followed by T7 Endonuclease I (NEB) treatment. The sequencing libraries were prepared using Rapid Barcodig Kit SQK-RBK004 and sequenced with MinION instrument using FLO-MIN106 flowcell (Oxford Nanopore).