GSM5596578: TY1_kr_a; Homo sapiens; ChIP-Seq
WT 9-12 cells were cross-linked with 1% formaldehyde for 10 min at RT. Cross-linking was stopped by 150 mM glycine for 5 min at RT. Cells were washed with PBS twice and collected using ChIP cell lysis buffer (10 mM EDTA, 1% SDS and 50 mM Tris-HCl pH 8.0). Cells was fragmented by sonication (range 200-500 bp). Immunoprecipitation was performed using 2 μg TY1/PanKcr/H3K18cr antibody. After elution and reversal cross-linking, samples were treated with RNase A for 30 min at 37℃. DNA was purified and sequenced on the Illumina NovaSeq platform. Libraries were prepared according to VAHTS Universal DNA Library Prep Kit for Illumina V3 Kit (Vazyme, # ND607-02).