GSM4908789: ELA_rep1; Rattus norvegicus; RNA-Seq
RNA was isolated using the Qiagen RNeasy Kit, according to the manufacturer's instructions. Libraries were constructed using the Illumina TruSeq v2 kit with minor modifications. Briefly, 1 µg of total RNA was used as input, and poly(A) selection was carried out according to the protocol. Modifications to the TruSeq v2 protocol included a fragmentation time of 3 minutes. Additionally, 8 cycles of PCR were run for the enrichment of DNA fragments. Adapters were chosen according to protocol recommendations. Quantification of the libraries was accomplished using the KAPA qPCR kit for Illumina libraries.