GSM6185850: F2_blue_sperm_flash_frozen; Ictalurus furcatus; RNA-Seq
AllPrep DNA/RNA Kit (Qiagen, CA, USA) The RNA integrity was evaluated with the LabChip GX Touch HT (PerkinElmer, MA, USA). The RNA library preparation was performed using NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, MA, USA). Enriched RNAs were fragmented for 10 minutes at 94 °C. The first strand cDNA synthesis, second strand cDNA synthesis, end repair and adaptor ligation according to the manufacturer's protocol. The library PCR amplification were performed with 16 cycle PCR. The RNA sequencing libraries were checked using the TapeStation 4200 D1000 ScreenTape (Agilent Technologies, CA, USA) and quantified by using Qubit 2.0 Fluorometer (Invitrogen, CA, USA).