GSM5325018: 597 (KO) Treg Thym; Mus musculus; RNA-Seq
Total RNA was isolated from T cells of thymus, spleen and lung sorted directly into RNA lysis buffer (RNeasy, Qiagen). RNA quality was controlled by Bioanalyzer (Agilent Technologies) RNA 6000 Pico kit (Agilent Technologies) regarding RIN > 9. First strand cDNA synthesis, library construction and indexing was performed according to manufacturer's guidelines (SMARTer Mouse TCR a/b Profiling Kit, Takara).