GSM4971793: TCF_rep2; Mus musculus; RNA-Seq
Total RNA was isolated using the PicoPure RNA Isolation Kit (Arcturus) following the manufacturer's instructions. Single-cell RNA-seq samples: Whole live cells were washed twice in 1x PBS + 0.04% BSA and immediately submitted to the Core for Single Cell sorting. The cells were first counted and measured for viability using the Vi-Cell XR Cell Viability Analyzer (Beckman-Coulter), as well as a basic hemocytometer with light microscopy. The barcoded Gel Beads were thawed from -80C and the reverse transcription master mix was prepared according to the manufacturer's instruction for Chromium Single Cell 3' v2 library kit (10x Genomics). Libraries were generated and sequenced by the University of Chicago Genomics Facility.