GSM6255126: WTC11-derived microglia (resting) - ATAC_rep2; Homo sapiens; ATAC-seq
Cells were harvested, washed with ice cold PBS containing protease inhibitor and nuclei were extracted prior to library construction. The standard ATAC-seq protocol was used, with the transposase reaction carried out for 30 minutes at 37C. 8-10 cycles of amplification were performed and dual size selection was performed with SPRIselect beads.