GSM5240632: cKO1-Treg cells (ATAC-seq); Mus musculus; ATAC-seq
50,000 CD4+CD25+YFP+ Tregs were isolated from 7-week-old chimeric female WT and cKO mice by FACS and washed once with 50 μl cold PBS buffer. Supernatant was discarded by centrifugation at 500 g, 4oC for 5 min. Lysed the cell pellet in 50 μl cold lysis buffer (10 mM NaCl, 10 mM pH7.4 Tris-HCl; 0.5%NP-40 and 3mM MgCl2) and centrifuge immediately at 500g, 4°C for 10 min. Transposition reaction and purification PCR amplification were performed using Nextera XT DNA Library Preparation Kit (Illumina, Cat. #FC-131-1096) and Qiagen MinElute PCR Purification Kit (Qiagen, Cat. #28004). Illumina Novaseq 6000 was used for sequencing.