GSM5134110: EMs rep2; Homo sapiens; RNA-Seq
[The collection and purification of GCs]  On the oocyte retrieval day, we collected GCs from patient follicular fluid. Only clear follicular fluid without blood or flushing solution was collected. Each follicular fluid sample in 15-ml disposable sterile tube was immediately centrifuged at 1000r/min for 10min. After removing the supernatant, the cellular precipitate was mixed 1:1 with PBS buffer, then was slowly added to the upper layer of 45% PercollTM (GE Healthcare, Sweden) separation solution, 1000 r/min, centrifugation for 20 min. then the middle ring-like layer (GCs) was collected. GCs from each participant were stored at -80°C for RNA extraction. RNA libraries were prepared for sequencing using standard Illumina protocols