GSM6182519: 21LP013; Homo sapiens; RNA-Seq
Total RNA was extracted from tissue by Trizol reagent (Invitrogen). The RNA quality was checked by Bioanalyzer 2200 (Aligent) and kept at -80℃.The RNA with RIN >8.0 is right for cDNA library construction. RNA libraries were prepared for sequecncing using standard illumina protocols