GSM5708885: Input_KO1; Mus musculus; OTHER
For the IP experiment, translating ribosomes were pulled down using anti-HA antibodies (BioLegend, 901513) and Pierce™ Protein A/G Magnetic Beads. Total RNA was extracted from immunoprecipitates using the Qiagen RNeasy extraction kit supplemented with β-mercaptoethanol. For both the IP and Input experiments, Poly(A) enrichment was done using Dynabeads Oligo(dT)25 beads. Both the IP and Input libraries were prepared using the ONT's sequncing kit SQK-DCS109, and barcoding expansion kit: EXP-NBD104. Libraries were multiplexed using the barcoding expansion kit and sequenced in a MinION flowcell (FLO-MIN106).