GSM6182661: ATAC_ProB_WT_Rep2; Mus musculus; ATAC-seq
Cells were extracted from bone marrow (femur and tibia of both hind legs) of Hdac7+/- and Hdac7fl/- mice. Cells were stained and sorted as pro-B cells (IgM-,CD19+,B220+, CD43+) and pre-B cells (IgM-,CD19+,B220+, CD43-). Fresh whole nuclei were used for chromatin transposition using Nextera Tn5 Transposase (Illumina). Two duplicates per condition were processed. DNA libraries were prepared as described in Buenrostro et al., 2015