GSM5487831: MEC-1; Bos taurus; RNA-Seq
Total RNA was extracted with standard chloroform extraction from ACADSB−/− and negative control cells. DNase I (NEB, Beijing, China) was used to treat total RNA and Trizol reagent to extract it (Invitrogen, USA). The RNA quality was checked using the Bioanalyzer 2200 (Aligent)and then stored at -80°C, and the cDNA libraries for each pooled RNA sample were created using the VAHTSTM Total RNA-seq (H/M/R) according to the manufacturer's instructions. The sequencing library of each RNA sample was prepared by using Ion Total RNA-Seq Kit