GSM5016614: JB1 ZFP57KO NPCs Rep2; Mus musculus; RNA-Seq
RNA was extracted from cultured NPCs by lysing the cells with the addition of the TRI reagent (Sigma-Aldrich)  and following the manufacturer's instructions ~1μg of high quality RNA (260/280 > 1.80) samples in biological duplicates were subjected to library preparation using TruSeq Stranded Total RNA Ribo-Zero Gold Kit (Illumina Inc. USA) and sequencing with 2x125bp paired end (PE) mode on Illumina HiSeq2500 platform (IGA Technology Services, Italy).