16S rRNA of mus musculus: cecum content of AHR mice fed with ctrl and EtOH diet
Total bacterial genomic DNA in cecum content was extracted as described. The V4 region of 16S rRNA genes was amplified and sequenced using the Illumina MiSeq platform (Illumina, San Diego, CA, USA) in Annoroad Gene Technology Co., Ltd (Beijing, China). The resulting 16S rRNA sequences were mainly analyzed based on QiiME2 workflows.