GSM5708933: 143B_shCtrl_2; Homo sapiens; RNA-Seq
After the total RNAs extracted from stable OS cells were separated by agarose electrophoresis and detected by Nanodrop, mRNAs were enriched with Oligo(dT) magnetic beads. The first cDNA fragment was generated by the random primer reverse, and the second cDNA was synthesized by adding dUTP. The double-stranded cDNA ends were repaired with A tails and connected to Illumina matching adaptors, and the final library was obtained by PCR amplification. An Agilent 2100 bioanalyzer was applied for quality control, qPCR was used for library quantification, and an Illumina HiSeq 4000 instrument was used for sequencing.