GSM5596025: γ-H2AX-IP-rep1; Mus musculus; OTHER
Lysates were clarified from sonicated nuclei and RNA-DNA-protein complexes were isolated with antibody. DNA was digested and RNA was extrated. Library preparation and sequencing were finished by LC-Bio Technology CO., Ltd., Hangzhou, China. The protocol is as follows: 1) RNA fragment and reverse transcription; 2) End repair, add A and adaptor ligation; 3) PCR; 4)Single strand separation and cyclization; 5) DNA nanoball synthesis; 7) Sequencing.