GSM6182679: H3K27me3_proB_KO_Rep2; Mus musculus; ChIP-Seq
Murine proB and preB lymphocytes were fixed using 1% formaldehyde and chromatin sheared to 300-500 bp in size using the Covaris E220 ultrasonicator. Resulting chromatin was incubated overnight with indicated antibodies. Purified immunoprecipitates were isolated and quantified by Qubit fluorometer. DNA sequencing libraries were prepared using the TRUESEQ ChIP kit (Illumina). Libraries were sequenced using HiSeqX 150+150PE platform from Magrogene Inc. Libraries were constructed using TRUSEQ ChIP kit from Illumina