GSM5016942: hPSC Meso-Hem; Homo sapiens; ChIP-Seq
For each ChIP experimental condition (day 0 for pluripotency, day 3 for neural and heamtopoietic differentiation protocols), 3×106 cells were collected and cross-linked using 1% formaldehyde. Chromatin was fragmented by sonication in buffer containing 1% NP-40 to obtain fragments of 250-500bp in length. Sonicated DNA was subjected to immunoprecipitation using anti-γH2A.X antibodies conjugated with Dynabeads® Protein G (Thermo Fisher Scientific). Immunoprecipitated DNA was further reverse cross-linked, purified and subjected to sequencing analyses. Libraries were prepared according to Illumina's instructions accompanying NEBNext Ultra II DNA library prep kit and sequenced with HiSeq 2500 instrument (Illumina).