GSM4907362: MYCNOS1 shRNA control Rep1; Homo sapiens; RNA-Seq
RNA was extracted from cultures by using TriPure isolation reagent (Roche Applied Science, Penzberg, Germany). The quality and quantity of RNA were determined by RNA6000 assay (Agilent Technologies, Santa Clara, CA, USA). Specimens with an RNA integrity number (RIN) >8.0 were used in this study. RNA libraries were prepared for sequencing using standard Illumina protocols RNA Libraries were constructed by using the TruSeq Stranded mRNA LT Sample Prep Kit