RNA-Seq in Setdb1KO mESCs treated with UNC
500 ng of total RNA was used for RNA-seq library construction. RNA-seq library was constructed by KAPA mRNA Hyper Prep Kit (KAPA BIOSYSTEMS) and SeqCap Adapter Kit (Roche) according to manufacturer instructions. The concentration of the RNA-seq library was quantified by KAPA Library quantification kit (KAPA BIOSYSTEMS). mRNA sequencing was performed on a HiSeq 2000 platform (Illumina).