GSM5209026: ATAC-seq_Flt3p_aLP_rep2; Mus musculus; ATAC-seq
Bone marrow was collected from the femur, tibia, and ilium (6 bones in total), crushed with the syringe plunger flange, passed through a 70um cell strainer, and resuspended in FACS Buffer (1X HBSS (Gibco), 0.25% BSA (Millipore-Sigma), 50 ng/mL DNase, and 5 mM sodium azide (Millipore-Sigma)). Bone marrow was lineage depleted using an autoMACS, stained for surface markers and viability, and individual bone marrow precursors were sorted into FACS Buffer. Library construction was performed as described in the published Omni-ATAC protocol: Corces, M. R. et al. An improved ATAC-seq protocol reduces background and enables interrogation of frozen tissues. Nat. Meth. 14, 959-962 (2017).