GSM6255115: WD KO2; Mus musculus; RNA-Seq
Whole aortas were harvested and separated from perivascular adipose tissue, then digested for 40 minutes at 37ºC in RPMI containing 450 U/ml Collagenase I, 125 U/ml Collagenase XI and 60 U/ml Hyaluronidase. Viable CD45+ and F4/80+ cells were sorted in a FACS Aria II and recovered in lysis buffer. cDNA was harvested using SMART-Seq v4 Ultra Low Input RNA Kit  following manufacturer's protocols.