GSM5135041: Children FluA Pool Rep2 [FluA-2019-BS]; Homo sapiens; Bisulfite-Seq
Nasal specimens were stored at -80⁰C and were brought to room temperature before pooling by age. DNA was isolated with a Qiagen DNeasy Blood and Tissue Kit. 100ng of DNA from each sample was sheared mechanically using a Covaris LE220-plus system to a length of 350 bp. Once the input DNA was at the proper fragment size, the samples were concentrated with a SpeedVac to a volume of 20µL. The samples then underwent bisulfite conversion with an EZ DNA Methylation- Gold kit Library was prepared according to the standard protocols of the ACCEL-NGS Methyl-Seq Library kit