rat 124, tMCAO, left (contralateral) hemisphere
tMCAO in experimental rats was initiated by endovascular occlusion of right middle cerebral artery using monofilament for 90 minutes followed by reperfusion. The anaesthesia was induced by 3% isoflurane and maintained by 1.5-2% isoflurane. Anesthesia was used only for the filament insertion and removal, while in between these procedures the rats were taken out of anesthesia. At 24 h after tMCAO operation, rats were decapitated. The subcortical structures of the brain were isolated from the left (contralateral) hemisphere. mRNA fraction from total RNA of rat subcortex was used for high-throughput RNA-sequencing (RNA-Seq). Sequencing was carried out using an Illumina HiSeq 1500 instrument. At least 10 million reads (1/50 nt) were generated.