3N_AE screen, t=36, replicate 1
Pooled plasmids were recovered from frozen cell pellets and HCM1 sequences were amplified by PCR with vector specific primers. PCR products were then purified by extraction from an agarose gel. TruSeq sequencing adapters were added via a second PCR using HCM1 specific primers fused to the TruSeq universal adapter or unique TruSeq indexed adapters. Libraries were then gel purified before sequencing.