RNA-seq for Pseudomonas aeruginosa LYSZa6
Magen HiPure Universal RNA Mini kits (MCBio, China) was used to extract total RNA following the manufacturers protocol. Extracted RNA was quantified using Qubit 2.0 (Thermo Fisher Scientific, MA, USA) and Nanodrop One (Thermo Fisher Scientific, MA, USA). Quality of RNA samples was assessed by Agilent 2100 system (Agilent Technologies, Waldbron, Germany). RNA libraries were constructed according to standard protocol using NEB Next Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, MA, USA). Ribosomal RNA depletion was carried out using Ribo-zero rRNA Removal Kit. cDNA was synthesized using NEB Next First Strand Synthesis Reaction Buffer. Paired-end RNA sequencing was performed on Illumina NovaSeq 6000 platform with read length of 150 bp.