WGS of S. cerevisiae CLY8 haploid
Multiplexed paired-end libraries were constructed from 2 g of genomic DNA purified using the Purgene kit (Qiagen). The genomic DNA was sheared using M220 focused-ultrasonicator (Covaris) and end-repaired using the End-it DNA End-repair kit (Epicentre Technologies). Common adaptors from the Multiplexing Sample Preparation Oligo Kit (Illumina) were then ligated to the genomic DNA fragments, and the fragments were then subjected to 18 cycles of amplification using the Library Amplification Readymix (KAPA Biosystems). The amplified products were fractionated on an agarose gel to select 600 bp fragments, which were subsequently sequenced on an Illumina NovaSeq 6000 using the Illumina GAII sequencing procedure for paired-end short read sequencing.