RNA-Seq of Neocalanus Flemingeri: 2017 KIP2 Adult Female 12 hour S14
Total RNA was extracted from individual females using QIAGEN RNeasy Plus Mini Kit (catalog # 74134) in combination with a Qiashredder column (catalog # 79654) and shipped on dry ice to the Georgia Genomics Bioinformatics Core (https://dna.uga.edu). Double-stranded cDNA libraries (NGS Stranded) were multiplexed and sequenced using Illumina Next-Seq 500 instrument (High Output Flow Cell, 75 bp, paired end).