GSM5708910: HRGECs-vehicle control1; Homo sapiens; RNA-Seq
Total RNA from cells was extracted using Trizol reagent. A total amount of 3 μg RNA per sample was used as input material for the RNA sample  preparations. Firstly, ribosomal RNA was removed by Epicentre Ribo-zero™ rRNA  Removal Kit (Epicentre, USA), and rRNA free residue was cleaned up by ethanol  precipitation. Subsequently, sequencing libraries were generated using the rRNAdepleted RNA by NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®  (NEB, USA) following manufacturer's recommendations.