GSM6255273: ECCITE-seq, replicate 1; Homo sapiens; RNA-Seq
MCF7 cells, transduced with a CROP-seq library targeting ER cofactors and selected for 6 days, were harvested by trypsinisation and libraries prepared using the 10x Chromium single cell 5' library and gel bead kit. Library prep was performed as per manufacturers instructions (chromium single cell 5' kit, 10x Genomics). Briefly, single-cell RNA-seq libraries were prepared iusing the following: Chromium Single Cell 5′ Library & Gel Bead Kit, PN-1000006; Chromium Single Cell A Chip Kit, PN-120236; Chromium i7 Multiplex Kit, PN-120262; and the Single-Cell V(D)J Reagent Kit (10X Genomics). To amplify gRNAs, 12 pmol RT primer (gd_RT_v4) was spiked into the library prep as described by Mimitou et al (2019) Nature Methods16:409-412