GSM5989834: HC1; Homo sapiens; RNA-Seq
CD19-expressing B cells were isolated from peripheral blood mononuclear cells with the CD1c (BDCA-1)+ Dendritic Cell Isolation Kit (Miltenyi) according to manufacturer's instruction. Cells were lysed in RLTplus buffer (Qiagen) supplemented with beta-mercaptoethanol (1%). Total RNA was purified using AllPrep Universal Kit (Qiagen), according to the manufacturer's instructions. RNA integrity was measured by capillary electrophoresis using a Bioanalyzer (Agilent), and the RNA 6000 Nano Kit (Agilent Technologies); all samples had RIN-score >7.0. RNA libraries were prepared according to the standard Illumina protocols