GSM5016607: RNAseL siRNA and Poly(I:C); Homo sapiens; RNA-Seq
Total RNA from human keratinocytes was purified using the RNeasy Mini Kit (Qiagen, 74106). RNA was incubated with DNase I to efficiently digest DNA prior to elution. RNA purity and quantity was calculated using a UV-Vis spectrophotometer (NanoDrop2000c (Thermo Fisher Scientific, ND-2000c). Total RNA quality was assessed by measuring 28S/18S ribosomal RNA ratios and scoring RNA Quality/Integrity Number (RQN/RIN) values via capillary electrophoresis using either 2100 Bioanalyzer (Agilent, Santa Clara, CA) or Fragment Analyzer CE (Agilent, Santa Clara, CA). TruSeq Stranded Total RNA LT Sample Prep Kit (Human Mouse Rat)/TruSeq Stranded Total RNA Sample Prep Guide, Part # 15031048 Rev. E