GSM5741432: F1-RNA-12-P6-DMSO; Mus musculus; RNA-Seq
Genomic DNA and total RNA were isolated from FACS-enriched germ cells and somatic cells of E18.5 fetal testes using the QIAGEN AllPrep Micro kit. RNA-seq Illumina libraries were contructed using Takara Bio's SMART-seq mRNA-seq kit, which involve cDNA synthesis using an oligo-dT primer. MBD-seq libraries were constructed using ThermoFisuer MethylMiner kit. RNA-seq [targeting mRNA and endogenous retrovirus RNA containing poly(A) tail]; MBD-seq [using a Methyl Binding Domain protein to enrich DNA fragments containing methylated CpG dinucleotides]