GSM5208636: Control [C-F7-NORAD]; Danio rerio; RNA-Seq
Total mRNA was extracted from GIT tissues using the miRNeasy Qiagen kit (Qiagen, Hilden, Germany). To prepare mRNA-Seq libraries the TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA, USA) was utilized; 100–200 ng of total input GIT RNA was used in accordance with the manufacturer's protocol. High through-put sequencing was performed at the University of North Carolina-Chapel Hill High Throughput Sequencing Facility, Chapel Hill, NC and the Queens University Belfast, Genomics Core Technology Unit. RNAseq was performed on Illumina Next SEQ 500 and HiSeq4000 instruments with each mRNA library sequenced to a minimum depth of ~25 million reads.