GSM5949207: nanoCT H3K27ac H3K27me3 rep 1; Mus musculus; OTHER
Nuclei were extracted using NP-40 / Digitonin buffer. Nano-Cut&Tag was performed with 1:100 diluted primary antibody and 1:100 diluted in house produced nanobody-Tn5 fusion. Tagmentation was perfmed for 1h at 37C in transposition buffer containing 10 mM MgCl2. Tagmented nuclei were processed using the 10x scATAC-seq kit according to manufactureres instructions skipping Step 1 "Transposition" and continuing from Step 2.0 "Generation and barcoding". The barcoded DNA was recovered from droplet emulsion according to Chromium NextGEM SingleCell ATAC ReagentKits v1.1(Step 3). Recovered DNA was tagmented using MeB (P7) loaded Tn5, purified using Zymo DNA clean and concentrator kit and library amplified by PCR