Virome of Vulpes vulpes feces: YANG12
For viral metagenomic analysis, twenty-four fecal samples from healthy adult red foxes were randomly collected from the same farm in Jilin Province, China, 2014. The twenty-four samples were randomly pooled into two supernatant pools, each including twelve samples. Each supernatant pool was filtered with a 0.45um filter to remove particles the size of bacterial and eukaryotic cells and the filtrates enriched in viral particles were treated with DNase and RNase to digest unprotected nucleic acid. The remaining total viral RNA and DNA were extracted by using the QIAamp viral RNA Minikit according to the protocol. Two libraries were then constructed using Nextera XT DNA Sample Preparation Kit (Illumina) and sequenced using the MiSeq Illumina platform with 250 bases paired ends with dual barcoding for each pool.