miRNA-Seq of tissue: adult female rat ovary
The sequencing procedure was performed strictly based upon standard steps which were provided by Illumina Inc., including library construction and sequencing test. TruSeq Small RNA Sample Prep Kits (Illumina, San Diego, USA) were used for miRNA library construction. Subsequently, the constructed miRNA library was sequenced on Illumina Hiseq2000/2500 (Illumina, San Diego, USA) with a single/paired-end 1-150bp read run. ACGT101-miR (LC Sciences, Houston, Texas, USA) which was developed independently by LC Sciences was used for data analysis. Firstly, removed the 3 adaptor and garbage sequences to obtain the clean data. Afterward, cleaned up the sequences within 18nt length. Finally, filtered these measured sequences with mRNA, RFam, and Repbase databases, and identified miRNA by comparing precursor and genome.