GSM5862431: F2 HCC 18M 006; Mus musculus; miRNA-Seq
Total RNA from was isolated using 50µl of mouse plasma. The sample volumes were brought up to 200 µl with water and then processed for RNA purification using the miRNeasy Serum/Plasma Advanced Kit from Qiagen. Synthetic miRNA mimics, UniSP2, UniSP4 were added to each sample before isolation A QIAseq miRNA Library Kit was used to build molecular-barcoding-enabled sequencing libraries