GSM4794617: 0077_c4h_ITGA6+_RNA-seq; Homo sapiens; RNA-Seq
Total RNA was extracted from ITGA6+ sorted primary testicular cells using a MagNA Pure LC instrument (Life Technologies). RNA was amplified using the Ovation RNA-Seq System V2 kit (NuGEN Technologies, lot no. 1307479-B) according to manufacturer's protocol. A total of 1 µg amplified cDNA was barcoded with a unique set of standard Illumina TruSeq indexed adapters by ligation and sequenced on an Illumina HiSeq 2000 high-throughput sequencer according to manufacturer's protocol.