GSM5208957: WT Atf1 Flag#2; Schizosaccharomyces pombe; ChIP-Seq
Lysates were generated by bead beating followed by sonication. Lysate were incubated with beads with antibody at 4C for 3 hours. Library for illumine sequencing was performed as previously described (ref) by following standard manufacture protocols, starting with 1-10ng immunoprecipitated DNA. Each library was generated by ligating with unique custom made adaptors. Libraries were pooled together and sequenced with Illumina HiSeq2500