GSM5016452: s_GV_Mov10l_KO_F77_r3.SE; Mesocricetus auratus; RNA-Seq
Total RNA was extracted from 6 – 12 fully-grown oocytes using Arcturus Picopure RNA isolation kit (Thermo Fisher) according to the manufacturer's protocol. RNA-seq libraries were generated using the Ovation RNA-Seq system V2 (NuGEN) followed by Ovation Ultralow Library system (DR Multiplex System, NuGEN) according to the manufacturer's protocol. cDNA fragmentation was performed on Bioruptor sonication device (Diagenode) with 30 s ON, 30 s OFF for 18 cycles on low intensity. Libraries were amplified by 9 cycles of PCR and sequenced by 100 nt single-end reading using the Illumina NovaSeq6000 platform.