GSM4907767: NF_1_LV; Homo sapiens; RNA-Seq
Frozen heart tissue samples were subjected to dry pulverization and RNA extraction was performed using the RNeasy Micro Kit (Qiagen) with on-column DNase treatment (Qiagen) according to the manufacturer's instructions.  Samples with ≥100 ng total RNA and RNA integrity numbers (RIN) ≥7 were used for sequencing. After passing the QC, total RNA was used for cDNA library preparation using a modified protocol based on the Illumina Truseq RNA sample preparation kit and the published method for strand-specific RNA-Seq. cDNA libraries were sequenced by tthe HiSeq platform (Illumina).