GSM5709417: lovo; Homo sapiens; miRNA-Seq
Centrifuge cell suspension, place the collected cells on the surface of the omentum and remove them, freeze them quickly on dry ice, and use Trizol reagent to obtain RNA. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols