GSM4794634: Whole kidney E14.5; Mus musculus; RNA-Seq
We harvested two kidneys at E14.5 and generated a single cell suspension using 0.05% trypsin at 37°C for 10 minutes. Kidneys were mechanically dissociated with pipetting at 5 and 10 min. 3% fetal calf serum in PBS was added to halt the trypsin. The cell suspension was filtered using a 40 mm filter and pelleted. The cells were resuspended in 90% FCS in DMSO and frozen Single-cell library preparation and sequencing was performed by GENEWIZ Inc. using the 10X Genomics Inc. Chromium 3' Single Cell v2 library preparation kit