WGS sequencing of dog gut microbiota: fecal samples
DNA was extracted using the Zymogen Quick-DNA Fecal/Soil Microbe 96 Mag Bead kit (Zymo Research, CA, USA) using Powerbead Pro (Qiagen, CA, USA) plates with 0.5mm and 0.1mm ceramic beads. Extraction controls included a no template control (water) and a characterized homogenized stool. All samples were quantified with Quant-iT Picogreen dsDNA Assay (Invitrogen, CA, USA). Subsequently, DNA amplification and library construction were performed with the Nextera XT DNA Library Preparation Kit (Illumina Inc, CA, USA).