GSM5862602: KO/Mock_12-3, rep2; Mus musculus; RNA-Seq
All cells were harvested and total RNA was purified by RNAeasy (Qiagen) with Dnase-treatment step. The library for RNA-seq was prepared from 400-500 ng of isolated RNA by using TruSeq Stranded Total RNA with Ribo-Zero kit (Illumina) according to the manufacturer's instructions. The pooled libraries with unique index sequences were sequenced using NovaSeq SP (Illumina) with single-end 100 bp reads.