RNA-seq of Ctenopharyngodon idellus: spleen
Briefly,  the spleen tissues of the three groups were collected at 5 days post-infection (dpi). Total RNA was extracted using TRIzol reagent according to the manufacturer's instructions (Invitrogen). Messenger RNA (mRNA) was enriched by Oligo (dT) beads and then fragmented using fragmentation buffer followed by reverse transcription into cDNA with random primers.