GSM5708858: RNA-Seq B6 Definitive Endoderm BioRep 44; Mus musculus; RNA-Seq
DE cells were lifted with accutase. RNA was extracted using trizol. After RiboGreen quantification and quality control by Agilent BioAnalyzer, 129-500ng of total RNA with RIN values of 9.5-10 underwent polyA selection and TruSeq library preparation according to instructions provided by Illumina (TruSeq Stranded mRNA LT Ki