Amplification of 16SrRNA gene of bacterial community from chemostat experiment to investigate degradation of permafrost
Bacterial DNA was extracted from filters using the ZymoBIOMICS DNA Miniprep Kit. Amplification of the 16S V4-V5 regions was performed using the sequence specific regions (515F-Y/926R) described in (Parada et al, 2016) using a two-step dual-index PCR approach specifically designed for Illumina instruments (Sequences provided are with already trimmed primers).