GSM6214675: PID42_1; Mus musculus; RNA-Seq
Mouse dorsal root ganglia were dissociated into a single nuclei suspension as described in Renthal et al. 2020 and Yang et al. 2021. Briefly, tissue was dissociated by dounce homogenization and clarified by ultracentrifugation through a Optiprep gradient based on a protocol (Mo et al. Neuron 2015) and modified in Renthal et al. 2020. inDrops libraries were constructued as described in Zilionis et al., Nat. Protoc. 2017.