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After the DNA sample was extracted, the DNA concentration and integrity were detected by electrophoresis. Total DNA was fragmented to about 350 bp by ultrasound. After end repair, A-ligation and adding sequencing linker, the unmethylated C in the DNA fragment was converted to U using bisulfite, and then the fragments were screened and PCR amplified, the DNA methylation library was prepared. After the library was qualified, the constructed library was sequenced. Qualified libraries were sequenced by Illumina HiSeq X ten platform in pair-end 150bp style.