Methylation analysis of Tf2 retrotrasposons in BPH
The predicted CpG island, located at the 5 end of the Tf2-retrotransposons, was PCR-amplified from the bisulfite-treated genomic DNA, extracted from stress-exposed BPH populations. The fragment thus obtained was gel purified and sequenced using the NEBNext Ultra DNA Library Prep Kit on Illumina MiSeq next generation sequencing system, using MiSeq Reagent Kit v2 500 cycles chemistry.