GSM6338491: Tumor1,Day80; Mus musculus; RNA-Seq
Total RNA was extracted from the tissues using Trizol (Invitrogen) .25µL~100µL of DEPC-treated water was added to dissolve the RNA. Subsequently, total RNA was qualified and quantified using a Nano Drop and Agilent 2100 bioanalyzer (Thermo Fisher Scientific, MA, USA). The final library was amplified with phi29 to make DNA nanoball (DNB) which had more than 300 copies of one molecular, DNBs were loaded into the patterned nanoarray and single end 50 bases reads were generated on MGIseq500 platform (BGI-Shenzhen, China)