GSM5484657: Col-0  WT  rep 3; Arabidopsis thaliana; RNA-Seq
Total RNA was isolated from 2-wk-old seedling grown on MS medium using RNeasy Plant Mini Kit (Qiagen), followed by TURBOTM DNase (Thermo Fisher) treatment. The quality and quantity of the RNA were assessed by NanoDrop and 2% agarose gel electrophoresis. Three biological replicates were carried out for each genotype, for a total of 6 samples. RNA libraries were prepared for sequencing using standard Illumina protocols; Libraries were multiplexed and sequenced on Illumina HiSeqTM 2500 to generate 100bp single end reads.