GSM4793124: SDS|SDS|Daughter [index704_505_S2B]; Homo sapiens; RNA-Seq
After first division, daughter cells were separated manually, washed in PBS and processed for cDNA synthesis and amplification using SMART-Seq V4 Ultra Low Input RNA Kit from Clontech (Catalog# 634892). Libraries were prepared using Illumina's Nextera XT DNA Library Preparation Kit (Catalog # FC 131-1096).  Briefly, 150 - 200pg of amplified cDNA was used for tagmentation followed by reduced cycle PCR amplification with indexed primers.  Libraries were quantified using an agilent bio-analyzer, pooled and subjected to next generation sequencing in a Hi-Seq 2500 under paired end 75bp sequencing conditions.