GSM5990268: HB48T; Homo sapiens; RNA-Seq
Fourteen fresh-frozen primary HBLs and five control liver samples (non-tumoral matched liver tissues) were surgically obtained from patients enrolled in Brazilian cancer institutions. Total RNA samples were isolated using the RNeasy Mini Kit (Qiagen, Hilden, Germany), according to the manufacturer's protocol. Microfluidics-based electrophoresis (Bioanalyzer, Agilent Technologies, USA) was performed to verify the RNA quality. The total RNA extracted from the samples was used to build cDNA libraries using the TruSeq®Stranded Total RNA LT- kit (with Ribo-Zero TM Gold) (Illumina, USA).