GSM5709282: MPC-UBTF-WT 2; Homo sapiens; RNA-Seq
CD34 cells were washed with cold 1X PBS, flash frozen on dry ice bath. Total RNA was then extracted from the frozen cell pellets using the Quick-RNA™ Miniprep Kit from Zymo (cat#R1054) following manufacturer's protocol.  Following RNA extraction, 500ng of total RNA were submited for library construction. RNA libraries were prepared for sequencing using standard Illumina protocols