GSM5990238: HPC-KO-2; Mus musculus; RNA-Seq
Mice were sacrificed either 30 minutes after a single-shock fear conditioning paradigm or directly from homecages. Whole brains were dissected out and rapidly snap-frozen using liquid nitrogen, then thawed on ice. Whole hippocampi (Experiment 1) were freehand dissected. Dorsal hippocampal sections were obtained using a brain matrix. Isolated hippocampi (whole [experiment 1] or 1mm-thick dorsal hippocalal sections [experiment 2]) were homogenized in trizol and total RNA was extracted using Trizol-Chloroform extraction. Total RNA quality was assessed on the Bioanalyzer platform using the RNA 6000 Nano assay (Agilent). mRNA was isolated from 300 ng total RNA using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (E7490L), and libraries were prepared using the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (E7770).