fecal microbiome from mice
Total DNA was purified from collected feces using the Quick-DNA Fecal/Soil Microbe Miniprep Kit (Zymo Research, Irvine, Canada, USA) according to the manufacturers instruction and subjected to shotgun sequencing. Sequencing libraries were prepared using the Illumina DNA Prep, (M) Tagmentation kit (Illumina, San Diego, California, USA), following the manufacturers guidelines. A cleaning step on the pool with 0.6 Agencourt AMPure XP beads was implemented. Sequencing was performed on a Novaseq600 S4 flowcell (Illumina, San Diego, California, USA)