GSM5808319: Hippocampus, Ctr; Mus musculus; Bisulfite-Seq
Hippocampus was removed, flash frozen on dry ice. Then genomic DNA was extracted using the Wizard Genomic DNA Purification Kit (A1125, Promega, WA, USA), treated with RNase A and quantified (NanoDrop 1000 Spectrophotometer, Thermo Fisher). About 3 μg DNA of each sample was fragmented by sonication. Subsequently, library preparation was performed using the SureSelectXT Mouse Methyl-Seq Reagent Kit (Agilent Technologies, CA, USA). DNA was bisulfite-treated using the EZ DNA Methylation-Gold kit. Sequencing libraries were assessed and quantified by Qubit® 3.0 Fluorometer and Agilent 2100. The libraries were then sequenced on a NovaSeq 6000 (Illumina) platform.