GSM5530416: Treg_Spleen_rep4 (SN_WT_SP6-9); Mus musculus; RNA-Seq
RNA was extracted with Qiagen's RNeasy micro kit from >100,000 FACS isolated cells as per instructions. For generation of sequencing libraries, 15ng of RNA (RIN value >7) were submitted to SPIA amplification (NuGen). Nugen Ovation RNA-Seq system V2 (for SPIA amplifications and cDNA generation) using Nugen protocol M01206v5, 2013; followed by Ovation Ultralow System V2 (for rest of library preparation) using Nugen protocol M01379v1, 2014.