GSM5324653: BAGCE_5; Cenchrus purpureus; RNA-Seq
Eight samples were selected, based on forage quality and lignin production. The highest nodes of C. purpureus stem were taken at Embrapa Dairy Cattle Napiergrass Germplasm Bank, in Coronel Pacheco, Minas Gerais. A total of 8 samples were stored in RNAlater solution (Life Technologies, Carlsbad, USA) at -20C. For RNA extraction, all samples were firstly ground in liquid nitrogen. The RNA extractions were performed using TRIzol reagent (Thermo Fisher, Waltham, USA), following Jordon-Thaden et al. (2015) protocol. All RNA samples were quantified by Nanodrop 1000 spectrophotometer (Thermo Scientific, Waltham, USA), and their integrity evaluated by Bioanalyzer RNA 6000 Nano kit (Agilent Technologies, Santa Clara, USA) according to manufacturers instructions. All samples presented RNA integrity number (RIN) over 6.4 and a minimum of 1000 ng of total RNA.  The cDNA library preparation was generated according to Illumina TruSeq Stranded mRNA Sample Prep kit protocol (Illumina, San Diego, USA), and RNA sequencing was performed using HiSeq 2500 sequencer (Illumina, San Diego, USA).