GSM5016542: 895 - Tumor; Homo sapiens; RNA-Seq
Cancer cells and adjacent stromal cells were microdissected using an Arcturus Pixcell IIE LCM platform and cancer and stromal cells were collected separately on CapSure™ Macro LCM Caps.  RNA was extracted from caputred tissue using a PicoPure™ RNA Isolation Kit. Amplified cDNA was then generated by using the SMARTer Ultra-Low-input RNA kit, version 4 followed by Nextera® XT DNA Library Preparation kit for sample barcoding and fragmentation per the manufacturer's protocols.