Stalk
Total RNA extraction was performed according to the manufacturer's protocol (Invitrogen). A total amount of 3 g RNA from each sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) following the manufacturers recommendations, and index codes were added to attribute sequences to each sample, followed by sequencing on an Illumina HiSeq platform.