GSM5767897: 2014-673_M2-2_Dyak_Caf_in_Dmel; Drosophila melanogaster; ChIP-Seq
Chromatin from whole flies (150 flies per technical replicate) was isolated according to standard modENCODE protocols using formaldehyde as the crosslinking agent and sonication. Immunpreciptation was performed using anti-FLAG antibody (Sigma) and protein G beads (GE Healthcare) Libraries for sequencing were prepared using the Ovation Ultra-Low Library System (NuGEN) and sequenced on the Illumina HiSeq platform by IGSB at The University of Chicago