WES of human normal: Sample 7ND
"germline DNA was extracted from either 650ul of saliva collected in an Oragene sample tube, or 200ul of blood collected in a PAXGene blood tube. Total nucleic acid was extracted with a Chemagic360 instrument using a source-specific magnetic bead protocol. Total nucleic acid was used for DNA library construction. and were quantified by a Quant-iT picogreen dsDNA reagent Kit (Life Technologies). Quality was confirmed using a LabChip GX Touch HT Genomic DNA Reagent Kit. Sequencing (WES and RNAseq) was performed on an illumina Hi-Seq 4000 system using patterned flow cell technology at TEMPUS, Inc. "