GSM4907392: Dko2 (RNA-seq); Mus musculus; RNA-Seq
RNA from cells was isolated with Trizol (ThermoFisher, Waltham, MA) following manufacturer's instructions. Isolated RNA was further purified using RNA clean up and concentrator kit (R1013, Zymo research) according to manufacturer's instructions. 40 ng of purified RNA was then used for preparation of RNA-sequencing libraries with the Nugen ovation RNA-Seq V2 system (now Tecan Genomics) according to the manufacturer recommended protocol. Samples were sequenced on Illumina Hiseq 2500 (single-end 50 bp reads)