Illumina NovaSeq 6000 paired end sequencing; RNA-seq: H3K9ac regulated by HDAC3 in trigeminal ganglion of SD rat trigeminal neuralgia model
RNA-seq: H3K9ac regulated by HDAC3 in trigeminal ganglion of SD rat trigeminal neuralgia model
The brainstem and trigeminal nerve roots on both sides were dissected Trigeminal ganglion tissues were stored in 1 mL TRIZOL (Thermo Fisher Scientific) and ground in liquid nitrogen. Next, 100 μL chloroform was added, and the cells were fully mixed and centrifuged at the highest speed at 4 °C for 10 min. The supernatant was transferred into a new tube, isopropanol was added to the same volume, and centrifuged at the highest speed at 4 °C for 10 min. The precipitate was washed with 75% cold ethanol and dissolved in the appropriate DEPC water. The concentration and quality of RNA were measured using a Nanodrop 2000 (Thermo Fisher Scientific) and an Agilent Bioanalyzer 2100 (Agilent). A total of 4 μg of RNA from each group was used for library preparation using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB) following the manufacturer's instructions