GSM5741303: 24hr_CT_op379; Mus musculus; RNA-Seq
Livers were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. The RNA sequencing libraries were prepared using Illumina TruSeq Stranded mRNA Sample Preparation Kit (Illumina Inc., USA), which produced directional RNA-seq libraries starting from 4 µg of total RNA according to manufacturer's instruction. The RNA libraries were quantified by qPCR and quality assessed with the 2100 Bioanalyzer HS DNA Kit.