GSM5709001: Acral melanoma, MOF-AM8-Toe-RNA; Homo sapiens; RNA-Seq
Upon collection, samples were immediately placed on ice and transferred for processing. Samples were digested using Miltenyi Tissue Dissociation Kit (Bergisch Gladbach, Germany) and enriched for live cells using FACS sorting prior to quantification and loading Sample were quantified and viability was analyzed using the Nexcelom Cellometer K2. The samples were then loaded onto 10X Genomics Chromium Single Cell Controller (10X Genomics, CA) to prepare single-cell RNA-sequencing libraries. Around 25,000 to 50,000 mean sequencing reads per cell were generated on Illumina NextSeq 500 instrument using v2.5 flow cells scRNA-seq