GSM5629072: BMSCs, Control3; Rattus norvegicus; RNA-Seq
Total RNA was extracted with TRIZOL according to the manufacturer's protocol.  RNA purity was checked using the NanoPhotometer® spectrophotometer. RNA concentration was measured using Qubit® RNA Assay Kit in Qubit® 2.0 Flurometer . RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system .A total amount of 1 μg RNA per sample was used as input material for the RNA sample preparations. RNA libraries were prepared for sequencing using standard Illumina protocols