GSM4907495: SN_input_Tamoxifen_E; Mus musculus; RNA-Seq
Dorsal cortex was removed, immediately immersed within ice-cold homogenization buffer, homogenized by hand within 10 minutes, and prepared for TRAP and synaptosome-TRAP as described (Sakers et al., 2017, Ouwenga 2017). RNA was purified using a Qiagen RNEasy MinElute kit per the manufacturer's instructions. RNA samples were reverse transcribed into cDNA and amplified using Smartseq v4 Ultra Low Input RNA Kit for Sequencing (Clontech) per the manufacturer's instructions. Then sequencing adaptors were added using the NEBNext Ultra II DNA Library Prep Kit for Illumina per the manufacturer's instructions.