GSM6181481: Y7543_K1; Mus; RNA-Seq
Single cells were obtained from asthmatic lung tissues by mechanical and enzymatic dissociation. After enzymatic digestion, the cells were placed in inactivation buffer (RPMI1640 + 10% FBS), filtered to remove any remaining tissue, the filtrate was centrifuged at 300g and the cell pellet was suspended in RBC lysis buffer, resuspended in inactivation buffer and viability is determined to be 85-95% before submitting for approximately 0.75 to 1x10^5  cells for scRNA-seq analysis. Library preparation was carried out using 10x Genomics Chromium controller (10x Genomics). Chromium 10x Genomics single cell library was sequenced as 150 base pair single-end reads on an Illumina NovaSeq 6000 (Illumina, Inc.), with cells from each of the two microchips sequenced across three flow cell lanes.