GSM5016454: s_GV_Mov10l1_HET_So811_r1.PE [Bisulfite-Seq]; Mesocricetus auratus; Bisulfite-Seq
10 fully grown GV oocytes (equivalent of 40 haploid genomes, 80 single-stranded DNAs after bisulfite conversion) were directly subjected to EZ DNA Methylation-Direct kit (Zymo Research) for bisulfite conversion with the following modifications: samples were digested with proteinase K at 50°C for 35 min and bisulfite conversion was performed as follows: 98°C 6 min, 64℃ 30 min, 95°C 1 min, 64°C 90 min, 95°C 1 min, 64°C 90 min. Bisulfite sequencing DNA libraries were prepared using EpiNext Post-Bisulfite DNA library Preparation kit according to the manufacturer´s protocol with 22 PCR cycles used for amplification. Final DNA libraries were sequenced by 250 nt pair-end sequenced on the Illumina NovaSeq6000 platform.