GSM6255096: esophageal squamous cell carcinoma, biol rep 5; unidentified; Bisulfite-Seq
Specifically, hippocampal tissues were incubated with tissue lysis buffer ( 10 mM Tris pH 8.0, 100 mM NaCl, 10 mM EDTA pH 8.0, 10% SDS) containing proteinase K at 56℃overnight, and DNA was extracted by phenol-chloroform and quantified by NanoDrop2000. The genomic DNA was bisulfite converted using EZ DNA Methylation-Gold™ Kit (ZYMO) according to the manufacturer's instruction. The bisulfite-converted DNA was amplified with primers specific for the NR2B gene promoter region.