GSM6255063: LTA4H_IP_2; Homo sapiens; RIP-Seq
The RBP-bound RNAs were isolated from the immunoprecipitation of anti-Flag using TRIzol (Invitrogen). Complementary DNA (cDNA) libraries were prepared with the KAPA RNA Hyper RNA binding protein connects the future Prep Kit (KAPA, KK8541) according the manufacturer's procedure and high-throughput sequencing of the cDNA libraries was performed on Illumina HiSeq X Ten platform for 150 bp paired-end sequencing.