16s sequence of gut microbiota from zebrafish
the raw pair-end readings were subjected to a quality-control procedure using the UPARSE-operational taxonomic unit (OTU) algorithm. The qualified reads were clustered to generate OTUs at the 97% similarity level using the USEARCH sequence analysis tool. A representative sequence of each OTU was assigned to a taxonomic level in the Ribosomal Database Project (RDP) database using the RDP classifier.