Illumina NovaSeq 6000 paired end sequencing; Amplicon sequencing of STING gene to validate CRISPR-Cas9 cut sites in PC9 cell line
Amplicon sequencing of STING gene to validate CRISPR-Cas9 cut sites in PC9 cell line
PC9 e.v. or sgSTING cells were harvested during routine sub-cultivation. Genomic DNA was extracted from  cells using the QIAamp DNA extraction kit (Qiagen). Amplicons of the STING gene were PCR amplified from DNA of PC9 e.v. and PC9 sgSTING cells. 500ng of PCR amplicon were purified using Ampure XP beads and prepared with the Illumina Nextera DNA Flex protocol (Illumina, USA) and post-PCR clean-up.