RRBS of folate deficient chick embryos: HH7
DNA samples were digested with methylation-insensitive restriction enzyme MspI; DNA fragments were end-repaired adding A-tail and ligated with the sequencing linker which all cytosine are methylated. DNA fragments from 150-300 pb were selected and treated with bisulfite by using EZ DNA Methylation Gold Kit (Zymo Research). Finally, PCR amplification was performed to obtain finally DNA library.