GSM5017668: WT naive CD8+, H3K27ac ChIP-Seq, rep3; Mus musculus; ChIP-Seq
Naïve CD8+ T cells were sort-purified from the spleen, crosslinked and immunoprecipitated with anti-H3K27ac antibody The DNA segments from DNase I digestion were end-repaired and ligated to indexed Illumina adaptors, followed by amplification by PCR with a low number of cells (18-21 cycles). The resulting libraries were seqeunced with Illumina Hiseq-2000 platform.