GSM5209096: 1372-RP-201; Cavia porcellus; RNA-Seq
Total RNA was isolated from the liver using RNeasy Plus Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's protocol. The isolated RNA samples to the Vanderbilt University Medical Center VANTAGE Core (Nashville, TN) for RNA quality determination and sequencing. At least 200 ng of DNase-treated total RNA with high RNA integrity was used to generate poly-A-enriched mRNA libraries, using KAPA Stranded mRNA sample kits with indexed adaptors (Roche, Indianapolis, IN). Library quality was assessed using the 2100 Bioanalyzer (Agilent), and libraries were quantitated using KAPA library Quantification kits (Roche). Pooled libraries were subjected to 150-bp double-end sequencing with the Illumina NovaSeq600 system (Illumina, San Diego, CA) according to the manufacturer's protocol. Bcl2fastq2 Conversion Software v2.20 (Illumina) was used to generate de-multiplexed Fastq files.