rat 129, sham-operation, left hemisphere
The sham-operated rats were subjected to a similar surgical procedure under anaesthesia (neck incision and separation of the bifurcation), but without tMCAO. At 24 h after sham-operation, rats were decapitated. The subcortical structures of the brain were isolated from the right hemisphere. mRNA fraction from total RNA of rat subcortex was used for high-throughput RNA-sequencing (RNA-Seq). Sequencing was carried out using an Illumina HiSeq 1500 instrument. At least 10 million reads (1/50 nt) were generated.