GSM5596378: AGAT wildtype mouse 5 [AGAT_WT_5_4692]; Mus musculus; miRNA-Seq
Total RNA, including miRNA fraction, was performed using QIAzol lysis reagent (QIAGEN). RNA integrity and the content of miRNAs was determined using the Agilent 2100 Bioanalyzer (RNA 600 Nano Chip, Agilent Small RNA Chip). RNA libraries were prepared using TruSeq Small RNA Sample Preparation Kits (Illumina, San Diego, CA, USA) according to the manufacturer's instructions.