GSM5726181: Granta519-ESR2 ChIP-seq rep 1; Homo sapiens; ChIP-Seq
Cells were crosslinked with 1% formaldehyde for 10 minutes at room temperature and nuclear chormatin fractions were separated and sonicated,  followed by immunoprecipitated with ESR2 anitboides (PPZ0506, R&D systems). Magnetic beads were used to pull down the immunoprecipitated DNA and separated by elution buffer containing proteinase K. Then ChIP DNA was purified with QIAquick PCR Purification kit (Qiagen). The libraries of ChIP DNA were prepared by using the kit NEB Next Ultra II DNA Library Prep Kit for Illumina following user instrcutions