GSM5209423: HU_g1_rep4 [TnseqHU22new]; Bacillus subtilis; OTHER
Genomic DNA extraction was performed. To ligate barcoded adapters, DNA was digested with MmeI and CIP purified. Adapters were then ligated to the ends and the samples were again purified. Quantitative PCR was performed with a subset of the samples to determine starting volumes for sequencing.