Single-cell RNA-Seq of healthy human control: adult male PBMC
PBMCs were thawed quickly at 37C and resuspended in RPMI media supplemented with 10% FBS. Approximately, 1,000-1,200 viable cells per microliter were loaded for capture onto the Chromium System using the v2 single-cell reagent kit (10X Genomics). Following capture and lysis, scRNA-Seq libraries were prepared from ~16k cells using a 10X Genomics Chromium device and Chromium Single Cell 3 Reagent Kit v3.1 according to manufacturers protocol. GEX (transcriptome) libraries were sequenced on a NovaSeq 6000 DNA sequencer (Illumina, Inc.) using V1.5 chemistry, generating ~160M GEX reads per sample. The raw data was processed using RTA 3.4.4.