RNAseq of C. felis adult salivary gland
Intact pairs of salivary glands were collected from adult Ctenocephalides felis females and pooled in RNAlater solution. The total RNA was isolated using RNAeasy RNA isolation kit (QIAGEN, USA) according to the manufacturer instructions and analyzed with an Angilent 2100 Bioanalyzer system (Agilent). Sequencing libraries were generated using NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB) following manufacturers and sequencing was performed in a Illumina Novaseq 6000 DNA sequencer