GSM5989658: ZWCH35; Mus musculus; RNA-Seq
Prostate samples were dissected and minced to small clumps, followed by enzymatic dissociation with 0.2% Collagenase/Hyaluronidase in DMEM/F12 media with 5% FBS for 3 h at 37°C. Tissues were digested with 0.25% Trypsin-EDTA for 1 h at 4°C, and filtered through a 40-mm cell strainer to obtain single-cell suspensions. Libraries were prepared for sequencing using standard Illumina protocols