WGS of homo sapiens : adult male balf
The concentration of 300ng of DNA was usedto prepare the whole Metagenome library (Library Prep Kitfor MGI high-throughput sequencing platform. Using restriction enzymes , 100-200bp size was fragmented and end-repaired, immediately ligated with the adaptor, and then, the library was enriched with 12 cycles of PCR. Using AgencourtAMPure XP beads (Beckman Coulter), purification was performed following manufactures instruction, and their quality was checked with Qsep100 fragment analyzer  before sequencing using in MGISeq 200.