GSM5207967: Ctl5_CD28 [primary CD4+ T cells]; Homo sapiens; RNA-Seq
Total RNA was extracted with the RNeasy Plus Micro Kit (QIAGEN), according to the manufacturer's instructions. cDNA was prepared with the SMART-Seq RNA kit (Takara Bio) for low-input RNA and DNA library preparation. We used the Nextera XT DNA Library Preparation Kit (Illumina) and the Nextera XT Index Kit v2 set A (Illumina), respectively, for idexing with low DNA input requirements.