GSM5016421: s_testis_Mov10l_WT_13dpp_F60-M2_r1.reseq [small RNA-seq]; Mesocricetus auratus; ncRNA-Seq
Total RNA was extracted from adult, 21dpp, 13dpp and 9dpp hamster testes using Sigma-Aldrich miR Premier micro RNA isolation kit according to the manufacturer´s protocol. Ribosomal RNA (rRNA) was depleted from RNA used for transcriptome analysis using Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat) (Epicentre) according to the manufacturer´s protocol. rRNA depletion was confirmed by 2100 Bioanalyzer (Agilent Technologies). For small RNA-seq analysis of testes, total RNA isolated from testes as described above was used for NextFlex Small RNA-seq v3 kit (Amplicon). Libraries were prepared according to the manufacturer´s protocol with 3´ adapter ligation overnight at 16 °C, 15 cycles used for PCR amplification and NextFlex beads used for size selection. RNA-seq libraries were sequenced by 75 nt single-end reading using the Illumina NextSeq500/550 or 100 nt single-end reading using NovaSeq6000 platform.