GSM5708567: Subject 1 DP ATMs; Homo sapiens; RNA-Seq
Samples were thawed at 37°C , run through QIAshredder columns for sample homogenization, and purified using an RNeasy Mini Kit (Qiagen). The University of Michigan Sequencing Core confirmed RNA integrity on an Agilent 2100 Bioanalyzer (RIN 7.9-10 after one freeze/thaw of purified RNA), and samples were then processed with the Pico Input Mammalian SMARTer® Stranded Total RNA-Seq Kit (Clontech).