HiSeq metagenomic reads of S. methanicola gut contents from Tube A
1 g of DNA extracted on 2/6/2012 was sheared into 200-300 bp fragments using an S2 Covaris. The fragments were end repaired and used for metagenomic library preparation using Illuminas TruSeq DNA Sample Preparation Kit (San Diego, CA,USA) with 12 cycles of amplification. Gel size selection was performed on the libraries to select fragments of 200-250 bp for paired-end 2x100 bp sequencing on a single lane of the Illumina HiSeq 2000 at Scripps Research (formerly The Scripps Research Institute; La Jolla, CA, USA). Reads uploaded are untrimmed and some contain the Illumina Universal adapter and TruSeq Adapter, Index 8 (GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC/GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATATCGTATGC).