GSM5240708: Unt_SNU-seq_rep3; Homo sapiens; OTHER
Cells were washed with cold PBS before lysing in Qiazol on ice. Samples were spiked with Saccharomyces cerevisiae RNA (0.01%). RNA was purified by addition of chloroform, vortexing and centrifuging for 15min, 4oc, 13000g. RNA was precipitated with isopropanol, washing three times in 75% ethanol, drying at RT and resuspending in RNase-free h2o. RNA was biotinylated and captured with streptavidin. A poly(A) tail was artificially added. QuantSeq 3' mRNA-seq library prep kit for ion torrent (Lexogen)