GSM5530549: KO uninfected PRO513A8; Mus musculus; RNA-Seq
For isolation of EGCs, the longitudinal smooth muscle layer and associated myenteric plexus were peeled off the wall of the smal intestineand subjected to enzymatic digestion to prepare a single-cell suspension. Single-cell suspensions were subjected to fluorescence activated cell sorting and live cells were isolated based on DAPI exclusion. Cells were quantified using the Eve Automatic Cell Counter and trypan blue. Approximately 10 000 cells were loaded into a 10x Chromium Chip G (10x 3' v3.1) and partitioned into GEMs using the 10x Chromium Controller. cDNA was generated using the manufacturers protocol -and quantified using the Agilent TapeStation (D5000 ScreenTape). Libraries were prepared according to the manufacturers guidelines and QC'd using the Agilent TapeStation and the High Sensitivity dsDNA Qubit.