Bulk RNA-Seq of xenopus laevis; adult; female left atrium
Total RNA was isolated from each of the biopsies using the Direct-zol RNA Microprep Kit (Zymo Research) including a DNase digestion step. This was followed by the assessment of the quality of RNA using Bioanalyzer RNA 6000 Nano Kit (Agilent). Subsequently, the rRNA depletion was carried out, followed by directional library preparation (NEBNext Ultra II RNA Library Prep Kit, NEB). The quality of the libraries were determined by using Bioanalyzer High Sensitivity DNA Kit (Agilent). Equimolar library pools based on the library quantification results of the NEBNext Library Quant Kit for Illumina (NEB) were sequenced in a paired end mode 75 cycles on a NextSeq 500 system (Illumina) using v2 chemistry.