Long-read RNA-seq of Phlegmariurus tetrastichus: roots, pooled tissue samples
Four tissue samples (new growth leaves, new growth stem, mature shoot, and roots) were acquired from a single, mature Phlegmariurus tetrastichus specimen. Following tissue dissection, samples were immediately snap frozen in liquid nitrogen. Total RNA was extracted using the Spectrum Plant Total RNA Kit (MilliporeSigma). RNA-seq libraries for PacBio SMRT sequencing were prepared following the Iso-Seq Template Preparation for Sequel Systems protocol (PacBio). Sequencing of pooled, barcoded libraries was performed on a PacBio Sequel System instrument using four SMRT cells with 20-hour movie lengths.