Aneurinibacillus sp. BA2021, whole genome shotgun sequencing
The initial gDNA concentration was quantified using the Qubit dsDNA HS Assay Kit (Life Technologies), and DNA quality evaluated using the Nanodrop 2000 (ThermoFisher Scientific). The sample's initial size was determined using electrophoresis (E-Gel SizeSelect 2% Agarose Gel; Invitrogen), which revealed a broad range smear for the sample spanning from approximately 1Kb  15Kb. As a result, following gDNA shearing (Covaris G-tube; Covaris Inc.), DNA fragments ranging from 6Kb to 10Kb were selected for using the Sage Science BluePippin automated size-selection instrument. The size of sample was verified using the Agilent 2100 Bioanalyzer (Agilent Technologies), and 150-250 ng of gDNA was used to enter into the SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences).