GSM6338485: Liver mitochondria, 65 week, bio rep 4; Mus musculus; RNA-Seq
The isolated and purified mitochondria were lysed in 1 ml TRIzol using the vendor's protocol. The samples were then treated with DNase I (Thermo Scientific) following the manufacturer´s instructions. For purification of the sample, the RNA Clean and Concentrator Kit (R1013, Zymo Research) was used Library was constructed using vendors protocol of PCR-cDNA Barcoding (SQK-PCB109) PCR steps adds barcodes to each reverse transcribed RNA (cDNA) sample. The 8 barcodes provided in the kit were used to multiplex individual samples on a single flow cell