GSM5767882: Zeus_KO_testis_rep1; Drosophila melanogaster; RNA-Seq
Using the ArcturusTM PicoPureTM RNA Isolation kit (Applied Biosystems, LOT 00665884) poly(A) mRNA was purified from total RNA, using oligo(dT)-attached magnetic beads; reverse-transcribed to double-stranded cDNA with random primers; end-repaired; and ligated with NEB adaptors for Illumina sequencing (HiSeq 4000, University of Chicago Genomics Core Facility)