GSM5135232: MHC I low/neg patient SCLC_1; Homo sapiens; RNA-Seq
Up to 10 consecutive FFPE 5µM sections were macrodissected or whole slide scraped and pooled into a single tube for downstream DNA and RNA isolation. DNA and RNA were co-extracted using the Qiagen AllPrep kit (Qiagen Cat No./ID: 80204). DNA and RNA were qualified and quantified using Nanodrop and Qbit to determine concentration and Agilent Bioanalyzer to determine quality. DNA and RNA samples with less than 100ng were excluded from downstream analysis. RNA sequencing libraries were prepared using the TruSeq Illumina Stranded Total Gold with RiboZero using 100 ng of total RNA. Libraries were sequenced on the Novaseq.