Small RNA sequencing of eight wines of Zeleni Sauvignon variety, group9
The isolation of small RNAs was performed by enrichment procedure using the mirVana miRNA Isolation Kit (Ambion, Life Technolgies). Using the Ion Total RNA-Seq kit, small RNA libraries were constructed. The first step was hybridization and ligation of the adapters, followed by reverse transcription, cDNA amplification and barcoding. cDNA libraries were analysed using Agilent 2100 Bioanalyzer (Agilent Technologies). Barcode-labeled cDNA libraries were sequenced using the Proton system (Life Technologies) according to the producer instructions.