GSM5708987: IR 3; Mus musculus; RNA-Seq
Total RNA was isolated using TRIzol reagent (Invitrogen, MA, USA).The concentration and purity of RNAs were determined by a NanoDrop® ND-2000 spectrophotometer (Thermo, Waltham, MA, USA). After rRNA depletion with the Ribo-Zero Magnetic Gold Kit (Epicentre, Inc., Madison, WI, USA), the RNA libraries were constructed using the TruSeq Stranded Total RNA Library Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer's instructions. RNA libraries were prepared for sequencing using standard Illumina protocols