GSM5241114: APL347; Homo sapiens; RNA-Seq
Bone marrow mononuclear cells from 18 centers were isolated after Ficoll–Isopaque density gradient centrifugation. Genomic mRNA were extracted from the patients' BM using the AllPrep PowerFecal RNA Kit in accordance with the manufacturer's instructions. After additional enrichment by purification with Ficoll, BM blasts were over 80%. The DNase I-treated total RNA preparations were qualitatively validated by Agilent RNA 6000 Nano Kit with the 2100 Bioanalyzer system, and libraries were prepared using the KAPA RNA HyperPrep kit in accordance with the manufacturer's instructions (KAPA). High-throughput sequencing was performed on the NovaSeq 6000 platform (Illumina, San Diego, CA, USA) in accordance with the manufacturer's paired-end 2 × 150 bp protocol.