GSM6182618: T47D, INPUT, TLE3; Homo sapiens; ChIP-Seq
Cells were fixed and immunopreciptated using Diagenode iDeal ChIP-seq kit for Transcription Factors per the manufacturers' instructions. For TLE3 immunopreciptations, cells were crosslinked using Diagenode ChIP Cross-link Gold prior to formaldehyde fixation. Library preparation was performed by Diagenode. Briefly, libraries were prepared using IP-Star Compact Automated System from both INPUT and ChIP DNA samples (starting amount of 3µg for IP and 1.5µg for INPUT) with the MIcroPlex Library Preparation Kit v2 (Diagenode #C05010013). Library amplification was assessed by qPCR, purified, and quantified with a Qubit dsDNA HS Assay Kit (Thermo Fisher Q32854). Fragment size was analyzed using High Sensitivity NGS Fragment Analysis Kit on an Agilent Fragment Analyzer.