GSM5240717: Unt_RNA-seq_rep3; Homo sapiens; RNA-Seq
Cells were washed with cold PBS and lysed in Qiazol. 2% of drosophila sg4 cells were added. RNA was extracted with chloroform, vortexing and centrifuging for 15min, 4oc, 13000g. RNA was precipitated with isopropanol and the pellet resuspended in 1x DNase buffer and 7.5U DNase (Qiagen). The pellet was DNase treated for 30min at 37oc. Again this was precipitated with isopropanol, washing three times in 75% ethanol, drying at RT and resuspending in RNase-free h2o. QuantSeq 3' mRNA-seq library prep kit for ion torrent (Lexogen)