GSM4907332: RGFP_NKp46pos_1; Mus musculus; RNA-Seq
isolation protocol: Intestines were isolated and PPs were removed. Tissue was cleaned, longitudinally opened and chopped into pieces of 2 cm, followed by two rounds à 15 min at 37°C epithelial cell dissociation using Hank's balanced salt solution without calcium and magnesium (HBSS-/-, Gibco) supplemented with 2% FCS, 10 mM HEPES buffer (Sigma), 1 mM DTT (ThermoFisher) and 5 mM EDTA (Sigma). After washing with HBSS-/-, tissues were digested using a lamina propria dissociation kit (Miltenyi) according to the manufacturer's instructions. Lymphocytes were further enriched on a 40%/80% Percoll (GE Healthcare) gradient.  Cells were sorted on a BD Aria II machine. FACS sort: Viable Lineage (CD19, CD11c, CD11b, F4/80,FceRIa, Gr-1)-CD45+CD3-RORgt-GFP+  expressing either NKp46 or CD4 RNA isolation protocol: RNA of cells was extracted using RNeasy Plus Micro Kit (Qiagen) and RNA quality was assessed by an Agilent 2100 Bioanalyzer. SMART-SeqII (ultra-low input RNA) libraries were prepared using Nextera XT DNA sample preparation kit (Illumina).