shotgun metagenome sequencing of obese adolescent human stool
DNA was extracted from 0.25 g of fecal material using the MoBio PowerMag Soil kit. DNA was quantified using the Quant-IT Picogreen kit. DNA libraries were then prepared using the TruSeq DNA PCR-Free WGS library preparation methodology. Libraries were then diluted and loaded onto an Illumina HiSeq 2500 for 75x75 paired end sequencing.