16S amplicons of soil microbes, McMurdo Dry Valleys
The 16S rRNA gene was amplified using Earth Microbiome Project primers, 515f-806r, for the V4-V5 region. Unique barcodes corresponding to unique samples were affixed. Then amplicons were normalized and pooled into a library which was sequenced on a 2x250 Illumina MiSeq run. Sequences were demultiplexed by barcode into unique files for each unique sample.