GSM5947012: PATC50 replicate 2; Homo sapiens; RNA-Seq
Total RNA was extracted from cells with Quick-RNA Miniprep Kit (ZYMO Research) according to manufacturer's protocols. The RNA-seq libraries were prepared with Ultra II Directional RNA Library Prep Kit for Illumina (NEB) according to manufacturer's protocols. The prepared RNA-seq libraries were sequenced on Illumina HiSeq 3000 platform.