GSM5862456: Cardiac myofibroblasts_siVgll3; Mus musculus; RNA-Seq
Total RNA from cardiac myofibroblasts treated with siRNA was extracted using the QIAGEN RNeasy plus Mini Kit (Qiagen) according to manufacturer's protocol. Library construction was performed by Macrogen Japan, using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina) and the libraries read on the Illumina NovaSeq 6000. RNA libraries were prepared for sequencing using standard Illumina protocols