Cecal contents from chickens treated with B. amyloliquefaciens
Total DNA from cecal content samples were extracted employing the Lysis and Purity kit (Shoreline Biome, Farmington, CT). Before extraction, samples were spiked wiith a low concentration of spiked with a low concentration of Vibrio harveyii. Libraries were prepared using Shoreline Biomes V4 16S DNA Purification and Library Prep Kit (Shoreline Biome, Farmington, CT). The V4 region of the 16S rRNA gene was PCR amplified using the 515F and 806R primers. Amplicons were sequenced using 2 x 150 bp paired-end kits on the Illumina iSeq platform. PhiX 50 pM was added to a final concentration of 5% into the amplicon library.