16S RNA-Seq of Mus musculus: HFpEF+NAM_1
Cecum samples were collected and snap frozen in liquid nitrogen immediately after mice were sacrificed. Genomic DNA was extracted using the ZymoBIOMICS DNA Microprep Kit (Zymo Research, USA) according to the manufacturers protocol. The V4 region of the 16S ribosomal RNA gene was amplified by PCR and underwent paired-end sequencing on an Illumina MiSeq (Illumina, San Diego, USA). Sequence depths are about 50,000 sequences per sample. The sequences were processed using the DADA2 pipeline in R which assigns taxonomy with the SILVA 132 database. The data were incorporated into QIIME 2 version 2019.10 after processing in R.