Whole genome sequencing of Saccharomyces cerevisiae HK9
From each colony derived from single zygotes (LSY3454-2 x JSC20-1), we generated a patch of cells growing at 30C on solid YPD medium (2% glucose, 2% peptone, 1% yeast extract, and 2% agar) and isolated DNA from the resulting patch without additional single-cell purifications. DNA was isolate using the Omega yeast DNA kit (Life Science Products) and was sequenced by the 150 bp-paired-end strategy using the Illumina NextSeq 500 platform.