GSM5017112: donor_37_F1; Homo sapiens; RNA-Seq
100 ng of total RNA were used to isolate mRNA using NEXTflex™ Poly(A) Beads (Bioo Scientific, Burleson Rd, Austin, TX, USA), as reported in the producer protocol. The obtained mRNA was used to prepare the RNA library using NEXTflex™ Rapid Directional qRNA-Seq™ Kit (Bioo Scientific, Burleson Rd, Austin, TX, USA), following the instructions. This kit contains a set of 96 distinct molecular labels on the sequencing adapters. Each label consists of an 8 nucleotide barcode tag. During the ligation reaction, each cDNA fragment end independently and randomly ligates to a single label from this pool of 96 adapters to result in a total of 96 x 96 = 9,216 possible combinations across both ends. For every clone sequenced, paired-end reads reveal the chosen label on each end along with adjoining cDNA sequence.