GSM5862590: ChIP_4; Mus musculus; ChIP-Seq
ChIP was done using SimpleChIP Enzymatic Chromatin IP Kit (CST, 9003) following manufacturer's protocol. Library was constructed by Novogene China Co. Ltd. Briefly, DNA fragments were undergone end repair. Adenylation of 3' ends of DNA fragments. Adaptor with hairpin loop structure were ligated to prepare for hybridization. Following purification, PCR was performed with Phusion High-Fidelity DNA polymerase, universal PCR primers and Index (X) Primer. At last, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system.