GSM5808308: ATAC.02_Scramble-rep2_ATAC_U937; Homo sapiens; ATAC-seq
ATAC-seq libraries were prepared following a published protocol (Liu et al. 2017). 100 k U937 cells are harvested. Nuclei were isolated, permeabilized, and tagmented using in-house produced Tn5 transposase; RNA was isolated using a standard Trizol extraction protocols and the result total RNA was treated with DNase I. For ATAC-seq, the resulted DNA fragments underwent two sequential 9-cycle PCR amplification and the fragments < 700 bp were purified using SPRI beads (Liu et al., 2017); RNA-seq libraries were generated using a standard ribo-zero in coupling with random primering strategy.