GSM5708767: RBM20-2; Mus musculus; RNA-Seq
Tibialis anterior muscles were isolated and flash frozen in liquid nitrogen. Muscles were pulverized under liquid nitrogen to prevent fiber type sampling bias then homogenized in Trizol before RNA was extracted with a Qiagen RNeasy kit using the standard protocol. RNA was treated with TURBO DNase (Ambion) to remove genomic DNA. Libraries were prepared using KAPA Stranded RNAseq Kits with RiboErase according to standard protocols.