Whole transcriptome PCR-cDNA sequencing with oxford nanopore MinIon of Drosophila melanogaster: DGRP_362 adult female whole flies
RNA was extracted from 12 female flies using TRIzol-Chloroform extraction then purified using Qiagen Rneasy Mini Kit column and Qiagen RNase-Free Dnase Set following manufacturer protocol. Libraries were prepared using cDNA-PCR Sequencing kit from Oxford Nanopore Technologies (SQK-PCS109) following manufacturer's instructions. Sequencing was done using MinION from ONT.