GSM5017117: K562_RPMI+dS_rep2; Homo sapiens; RNA-Seq
After 24 hr incubation, RNA was harvested using the miRNeasy Mini Kit (QIAGEN 217004) according to manufacturer instructions. 700 ng total RNA was used to generate mRNA libraries using the TruSeq Stranded mRNA Library Preparation Kit (Illumina 20020594). Libraries prepared from each sample were quantified using the KAPA Library Quantification Kit (Roche KK4824) and then pooled at equimolar ratios. Following denaturation, 40 bp single-end reads were generated on a HiSeq 2500 (Illumina).