GSM5208921: Sham capEC 3; Mus musculus; RNA-Seq
Total RNA was isolated from the sorted cells with RNeasy Plus Micro kit (Qiagen) and the quality of RNA was checked by Bioanalyzer RNA 6000 pico assay (Agilent, California, USA). Sequence library was prepared using SMARTer® Stranded Total RNA-Seq Kit v2-Pico Input Mammalian (634411, Takara Bio USA, Inc.). After library preparation and quality check, the double-stranded-cDNA was sequenced on NextSeq 500 (SY-415-1001, Illumina) using (read one-index reads-read two, bp): 75-8-8-75 setup.