RNA-seq of Homo sapiens; adult male: Sample Right_Lower_Lung_Normal_RNA
"Following pathologic review of all samples to confirm presence of tumor or normal tissues (in the case of nonadjacent matched normal, defined as >1 cm from the tumor margin), samples were submitted for nucleic acid isolation. Deidentified snap-frozen (SF) and formalin-fixed, paraffin-embedded (FFPE) tissue samples were sent to the UNC Lineberger Comprehensive Cancer Center Translational Genomics Lab for nucleic acid isolation using the Maxwell 16 MDx Instrument (Promega, AS3000).  RNA was extracted from approximately 10 mg of SF tissue using the Maxwell 16 LEV simplyRNA Tissue Kit (Promega, AS1280) and from FFPE slides using the Maxwell 16 LEV RNA FFPE Kit (Promega, AS1260) following the manufacturer's protocols (Promega, TM351 and TM408, respectively). RNA quality was measured using a NanoDrop spectrophotometer (Thermo Fisher Scientific, ND-2000C) and a TapeStation 4200 (Agilent, G2991AA). RNA concentrations were quantified using a Qubit 3.0 fluorometer (Life Technologies, Q33216). RNA libraries were stranded and sequenced via 150-bp paired-end reads. The RNA isolated from FFPE samples was first treated with a Ribo-Zero Magnetic Kit for rRNA removal (Illumina) prior to library preparation. RNA-Seq were performed on an Illumina NovaSeq 6000."