16s amplicon of Nasonia vitripennis, adult female
Prior to extraction, each individual was put in 1.5 ml tube and rinsed with 1ml 70% ETOH once, 1ml 10% bleach once, and finally sterile water 1ml twice. Then sampleswere frozen in liquid nitrogen with additional mechanical homogenization. All samples were processed for DNA extraction using the DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany) according to the manufacturers instructions. Samples were then quantified using the dsDNA HS Assay kit on the Qubit 2.0 Fluorometer (Life Technologies).