GSM5208216: mESC_Hi-C_DMSO_rep2; Mus musculus; Hi-C
DNA extraction was carried out using standard protocol and samples were processed according to the manufacturers' instructions using the Arima Hi-C Kit (Arima Genomics #A410030) Illumina compatible libraries were prepared using a modified version of the KAPA Hyper Prep Kit (KAPA Biosystems #07962347001). Quality control steps were performed to assess the fraction of proximally ligated DNA labeled with biotin, and the optimal number of PCR reactions needed to make libraries. The libraries were loaded onto an Illumina S2 flow cell (Illumina, USA) and paired end sequencing (2 x 50bp).