RNA-Seq of Daphnia magna treated with 25C & high food concentration 5
Total RNA were extracted using RNeasy Plus Micro kit (Product ID: 74034, QIAGEN, Hilden, Germany). The mRNA were isolated from the total RNA using NEBNext Poly(A) mRNA magnetic isolation module (Product ID: E7480, New England Biolabs, Massachusetts, USA), following the standard protocol provided by the manufacturer. Transcriptome library preparation procedures were performed following the standard protocol of NEBNext Ultra II RNA Library Prep Kit for Illumina (Catalog #: E7770, New England Biolabs Massachusetts, USA) with some modifications. The enrichment step for the library preparation was extended from 20 to 25 cycles to ensure an adequate amount of DNA for the sequencing. The Illumina MiSeq (Illumina, California, USA) with 150 cycles single read was used for the sequencing.