RNA -seq of Aspergillus nidulans: lreA in blue light-2
To construct the library, mRNA was enriched by oligo dT selection or rRNA depletion. Afterwards, the RNA samples were further fragmented and re-verse-transcribed to double-strand cDNA by N6 random primer. The cDNA was subjected to end-repair and then 3 adenylated, where was further ligat-ed with adapter. PCR amplification was performed to enrich the cDNA tem-plate. The PCR product was denatured by heat and then the single strand DNA was cyclized with splint oligo and DNA ligase.