NextSeq 500 sequencing; Chromatin IP sequencing of the nuclear factor HMGB1 in livers from mice after a metabolic stress either induced by a 12-week high fat diet consumption or a fasting (6hours)-refeeding (8hours) challenge.
Chromatin IP sequencing of the nuclear factor HMGB1 in livers from mice after a metabolic stress either induced by a 12-week high fat diet consumption or a fasting (6hours)-refeeding (8hours) challenge.
Liver from control (flox) or Hmgb1 liver knockout mice after a metabolic stress either induced by a 12-week high fat diet consumption or a fasting (6hours)-refeeding (8hours) challenge. Briefly, frozen liver biopsies (100-200 mg) harvested from HMGB1fl/fl and HMGB1Î”Hep mice under CD, upon HFD60% or after F/R, were minced and fixed at room temperature in PBS-1% formaldehyde (Sigma-Aldrich, 47608, Saint Louis, MO, USA) for 20 minutes. After sonication, chromatin immunoprecipitation was performed using anti-HMGB1 antibody (Abcam, ab18256, Cambridge, UK).  Immunoprecipitated DNA was subjected to library preparation using NEBNext DNA Ultra II kit.