P651_probioticmixture_batch_15
DNA was extracted following a phenol-chloroform-isoamyl alcohol protocol and precipitated with polyethylene glycol. Finally, the DNA was washed with 70% alcohol and diluted in DNase/RNase/protease-free water. Samples were shipped to CosmosID (CosmosID Inc., Rockville, MD, USA) for shallow-shotgun sequencing analysis. For this purpose, DNA libraries were prepared using the Illumina Nextera XT library preparation kit, with a modified protocol. Library quantity was assessed with Qubit (ThermoFisher). Libraries were then sequenced on an Illumina HiSeq platform 2x150bp at 3M total reads.