Illumina HiSeq 2500 sequencing; Transcriptomic analysis of undifferentiated and differentiated NMR OPCs.
Transcriptomic analysis of undifferentiated and differentiated NMR OPCs.
OPCs were isolated from NMR pups at postnatal day 2, passed once after 14 days, and the RNAs were collected using Trizol 5 days after the passage. Each sample was collected from a different litter. RNA from the differentiated NMR OPCs was collected 7 days after inducing differentiation. The RNA samples were extracted using Trizol reagent. RNA samples were sent to the Genomics Research Center of the University of Rochester for RNA-Seq. The TruSeq RNA Sample Preparation Kit V2 (Illumina) was used for library construction.