GSM5530701: RNA-seq cKO CB 1; Mus musculus; RNA-Seq
RNA was extracted from enriched fractions of Spc, RS or isolated CBs with the Trisure reagent (Bioline) using standard protocols. Isolated RNA was analyzed using NanoDrop (Thermo Scientific) and Bioanalyzer (Agilent). 1) CB RNA-seq: Total RNA libraries without ribosomal RNA removal were prepared by NEBNext® Ultra™ Directional RNA Library Prep Kit without fragmentation. Sequencing was performed with NovaSeq6000 (PE150) platform (Illlumina) at Novogene (www.en.novogene.com). 2) RNA-seq of Spc and RS samples: Libraries were constructed with Illumina TruSeq Stranded mRNA library preparation kit and sequenced with HiSeq3000 (read length 2 x 75 bp) sequencing system (Illumina, San Diego, CA, USA) at the Finnish Functional Genomics Centre (https://bioscience.fi/services/functional-genomics/services/). 3) SmallRNA-seq of Spc and RS samples: Libraries were constructed with QIAseq miRNA library preparation kit and sequenced with HiSeq2500 Rapid run sequencing platform (1 x 75bp) at the Finnish Functional Genomics Centre.