GSM5135776: 2D2 WT CNS Th17 cells_rep2; Mus musculus; RNA-Seq
RNA was extracted from splenic and CNS-infiltrating CD4+ T cells at the peak of disease, and from Th17 cells transduced with empty virus control or Egr2-expressing retrovirus under non-pathogenic [Th17(b)] or pathogenic [Th17(23) and Th17(alt)] conditions after brief 4-hr PMA and ionomycin restimulation, using QIAshredder columns and RNase Plus Mini kit (Qiagen). 26 mRNA-Seq samples were pooled and sequenced on HiSeq4000 using Illumina TruSeq Stranded mRNA Library Prep and paired-end sequencing.