16SrRNA-V3-V4-Amplicon of bee gut metagenome: 16S_MRG.2
Dissection of bee gut, Total DNA extraction using the kit NucleoSpin Soil (Macherey-Nagel) preceded by a proteinase K treatment for 4 hours at 56C, 250 bp paired-end amplicon sequencing at Novogene Corporation Inc (Sacramento, CA, USA)