EpiWT_1dwound_Atacseq
WT mice were wounded with 6mm punch biopsy tools, 1 day later, wounds or skin from unwounded region are excised and treated with trypsin to isolate epidermal keratinocytes.  Live, single epidermal stem cells were sorted based on markers (CD140a-CD31-CD117-CD45-Itga6HiCD34-Sca1+DAPI-) directly into TRIZOL.  Total RNA (10-30ng)was isolated and submitted for RNAseq.mouse skin are excised and treated with trypsin to isolate epidermal keratinocytes.  Live, single epidermal stem cells were sorted based on markers (CD140a-CD31-CD117-CD45-Itga6HiCD34-Sca1+DAPI-). Nuclei were extracted and genomic DNA was transposed per protocol outlines in doi::10.1038/nmeth.2688