WGS of L. plantarum: D. melanogastor gut
The DNA was extracted using the Qiagen DNeasy Powerlyzer Microbial Kit. DNA was then fragmented to 500 bp using NEBNext dsDNA Fragmentase (M0348L) and end-repaired with NEBNext Ultra II End Repair/dA-Tailing module (E7546L). Fragments were ligated to Illumina sequencing adapters with NEBNext Ultra II Ligation Module (E7595L) and size selected to roughly 500 bp. The ligated target sequences were enriched with KAPA Library Amplification kit (KK2621), again size selected to 500 bp, and normalized to 5ng/uL using Qubit measurements. The library was then sequenced on a partial lane of Illumina HiSeq 2500 using 250bp paired-end sequencing.