GSM5767888: 2014-1004-A1_Dsim_Zeus_in_Dmel; Drosophila melanogaster; ChIP-Seq
Chromatin from whole flies (150 flies per technical replicate) was isolated according to standard modENCODE protocols using formaldehyde as the crosslinking agent and sonication. Immunpreciptation was performed using anti-FLAG antibody (Sigma) and protein G beads (GE Healthcare) Libraries for sequencing were prepared using the Ovation Ultra-Low Library System (NuGEN) and sequenced on the Illumina HiSeq platform by IGSB at The University of Chicago