GSM5595974: HNSCC29 Mono; Homo sapiens; RNA-Seq
Monocytes were isolated from HNSCC patient PBMCs based on CD14+ and HLA-DR+ FACS sorting. Dry pellets using liquid nitrogen and storage at -80°C. Total RNA was extracted from cell pellets using RNeasy Plus Micro Kit (Qiagen) according to manufacturer's instructions. 30ng of RNA per sample was used for the preparation of the RNA sequencing libraries using the KAPA RNA HyperPrep Kit with RiboErase (KAPA Biosystems).