GSM5017755: Tamm-2 [RNA-seq]; Canis lupus familiaris; RNA-Seq
Total RNA was extracted from cell or animal tissue by Trizol reagent (Invitrogen) separately. The RNA quality was checked by Bioanalyzer 2200 (Aligent) and kept at -80℃.The RNA with RIN >8.0 is right for miRNA purification or cDNA library construction. miRNA was purified by miRNeasy Mini Kit (Qiagen) and the purification result was validated by Gel electrophoresis. Hiseq Sequencing Protocol