18S rRNA sequencing of seawater sample
500 mL of seawater was collected on a 0.22um Durapore filter and flash frozen in liquid nitrogen. DNA was extracted using a QIAGEN PowerWater DNA kit and the V4-5 region of the 16S rRNA gene and V4 region of the 18S rRNA gene were amplified with fusion primers. Cleaned products were pooled and sequenced using 300 bp paired-end methods on an Illumina MiSeq.