GSM5239572: Aged T0-2; Mus musculus; RNA-Seq
GFP+/DAPI- satellite cells were sorted by Florescence-Activated Cell Sorting (FACS) directly into SMART-Seq (Takara) reaction buffer composed of lysis buffer, water and Rnase inhibitors. cDNA was generated using SMART-Seq HT kit (Takara, cat. #624456). Illumina adapters were added using Nextera XT (Illumina, cat. #FC-131-1024, FC-131-1001)