GSM5241841: Cerebral Cortex_CVI_LCMV_Rep3; Mus musculus; RNA-Seq
At day 20 following injury, mice were perfused with normal saline, and a 3 x 3 x 3 mm cube of injured tissue from the Cerebral cortex was harvested and snap frozen using dry ice. The tissues were stored at -80oC until use. Total RNA was extracted with Qiagen kit following the manufacturer's protocol, and RNA quantity and integrity was assessed by the Bioanalyzer (Agilent, Santa Clara, CA). 500 ng of total RNA was used in conjunction with the TruSeq® Stranded Total RNA Library Prep kit (Illumina, San Diego, CA). The libraries quality was checked by the Bioanalyzer and quantitated by the Qubit (ThermoFisher Scientific). Equimolar quantities from each sample library was pooled and run on a Highoutput Next-Seq550 kit.