GSM4907388: LAB_LAB_25; Homo sapiens; RNA-Seq
Human left atrial tissue was obtained from a 75-year-old deceased white male organ donor who died of stroke. The brain dead donor had a medical history of atrial fibrillation, hypertension, pneumonia, and hypercholesteremia, with a body mass index of 24.4. The heart and lungs were clinically rejected because of poor organ function. The pulmonary artery was perfused with 4 liter of Perfadex (XVIVO Perfusion Inc., Englewood, CO) prior to explant and stored on ice for 5 hours. The heart and lung block was procured in the same manner as our clinical transplant team. The left atrium was photographed, cut into regional fragments, labeled, and snap frozen in liquid nitrogen and stored at -80°C until RNA extraction. Tissue was homogenized in one mL of TRIzol® (Invitrogen) with sterile Omni Tip Disposable Generator Probes.  RNA was isolated from the homogenate following the TRIzol method by the manufacturer's protocol.  The RNA pellet was dried and re-suspended in 80 µl of RNase-free water; the concentration was determined by Nanodrop spectrometry (OD260 nm) and stored at -80°C. Library generation for RNA-sequencing was done at the University of Chicago Genomics Facility using standard Illumina protocols.