GSM4792937: MT_HF2; Daphnia magna; RNA-Seq
For RNA-seq sampling, 5 daphniids were cultured individually in each condition until producing 2nd clutch (day 12). Eggs were removed before homogenization for RNA extraction by using Sepasol-RNAI solution (Nacalai Tesque, Kyoto, Japan) and purified by phenol-chloroform. Each treatment was repeated 3 times for triplicates of sampling. According to QC report from Novogene service, all samples passed agarose gel electrophoresis test and Agilent 2100 analysis test. All libraries were constructed following Novogene's protocol (jp.novogene.com). According to their service description, mRNA was first enriched using oligo(dT) beads, fragmented, followed by cDNA synthesis using random hexamers. After terminal repair, A overhang and sequencing adaptor were added. Finally, cDNA library were completed through size selection and PCR enrichment and then sequenced by Illumina sequencer.