GSM6254834: 450905, 7, Mild, Positive [scATAC-Seq]; Homo sapiens; ATAC-seq
PBMCs were thawed and nuclei were extracted as for ATAC-seq. The single-cell suspensions of scATAC-seq samples were converted to barcoded scATAC-seq libraries using the Chromium Single Cell 5′ Library, Gel Bead and Multiplex Kit, and Chip Kit (10x Genomics). The Chromium Single Cell 5′ v2 Reagent (10x Genomics, 120237) kit was used to prepare single-cell ATAC libraries according to the manufacturer's instructions. Quality was assessed using Agilent DNA tape screen assay.