GSM5709548: shDDX21-2 treated, differentiating keratinocytes, bio rep 1; Homo sapiens; RNA-Seq
For RNA-seq, RNA was extracted for sequencing using an RNeasy Plus kit (QIAGEN, 74136). For mRNA-seq splicing analysis, total RNA was extracted using the RNeasy Plus kit (QIAGEN, 74136), followed by poly(A) enrichment and library preparation using the NEB Ultra II (non-directional) kit (E6111). Libraries were sequenced on a NovaSeq 6000, PE150, with ~30 M reads for each sample.