bulk RNA seq of integrin beta1 KO A549 cells_rep3
RNASeq libraries were prepared using 300 ng of RNA and the NEBNext Ultra II RNA Library Prep kit (NEB #E7760L) per manufacturers instructions, with mRNA enriched via poly-A-selection using oligoDT beads. The RNA was then thermally fragmented and converted to cDNA, adenylated for adaptor ligation and PCR amplifed. Individual libraries were assessed for quality using the Agilent 2100 Bioanalyzer and quantified with a Qubit Fluorometer. The adapter ligated material was evaluated using qPCR prior to normalization and pooling for sequencing. The libraries were sequenced using the NovaSeq 6000 with 150 bp paired end reads. RTA (version 2.4.11; Illumina) was used for base calling and data QC was completed using MultiQC v1.7 by the Vanderbilt Technologies for Advanced Genomics (VANTAGE) core (Vanderbilt University, Nashville, TN).