ATAC-Seq of Amia calva: Stage 20-21 whole embryo
Whole embryos were dissociated into a cell solution with 0.125% collagenase at 37C for 20-40min until completely dissociated. Live cells were strained through a 100uM filter and counted on an improved Neubauer chamber staining with trypan blue to label dead cells. Aliquots of 100K cells were collected and lysed per sample for DNA transposition and PCR amplification. Before sequencing, Agencourt AMPure XP Beads were used to clean up PCR products according to manufacturers instructions. ATAC-Seq libraries were sequenced with Illumina NextSeq v2 High Output flow cell in a 2x75bp paired end format using a 150 cycle v2 NextSeq 500 reagent cartridge