Shotgun metagenomics of Autotrophic NRFeOx culture: short reads
Liquid cultures were filtered with 0.2 m pore-size fand subsequently placed in 2 mL centrifuge tubes and frozen. DNA was extracted using FastDNA SPIN Kit according to the manufacturers instructions. Library was prepared with1 g DNA using TruSeq DNA PCR-Free (Illumina). The library was sequenced on two flow cell lanes with 2 x 150bp using NovaSeq 6000.