GSM5239694: Human kidney sample - snATAC-seq_rep2; Homo sapiens; ATAC-seq
Kidneys were minced and lysed in 2 mL lysis buffer for 15 min. Tissue lysis reaction was then blocked by adding 10 mL 1x PBS into each tube, and solution was passed through a 40 μm cell strainer. Cell debris and cytoplasmic contaminants were removed by Nuclei PURE Prep Nuclei Isolation Kit (Sigma, NUC-201) after centrifugation at 13,000 RPM for 45 min. Libraries were prepared according to 10x Genomics's instructions (CG000209) accompanying the Chromium Next GEM Single Cell ATAC Reagent Kits v1.1. Single nuclei ATAC-seq