GSM5135751: Lung tissue - Bleomycin_rep1; Rattus norvegicus; RNA-Seq
Total RNAs were isolated from lung using TRIzol (Invitrogen, Carlsbad, CA, USA), then the mRNAs were enriched by Oligo(dT) beads (Epicentre). The enriched mRNAs were fragmented into short fragments using fragmentation buffer and reverse transcribed into cDNA with random primers. Second-strand cDNA were synthesized by DNA polymerase I, RNase H, dNTP and buffer. Then the cDNA fragments were purified with QiaQuick PCR extraction kit, end repaired, adenylated, and ligated to Illumina sequencing adapters. RNA libraries were prepared for sequencing using standard Illumina protocols