GSM5133957: wt_1; Mus musculus; RNA-Seq
RNA was isolated from sorted smooth muscle cells using the miRNeasy micro Kit (Qiagen) combined with on-column DNase digestion (DNase-Free DNase Set, Qiagen) to avoid contamination by genomic DNA. CAGE library preparation, sequencing, mapping and motif discovery analysis were performed by DNAFORM (Life Science Research Center, Japan). RNA quality was assessed by Bioanalyzer (Agilent) to ensure a RIN (RNA integrity number) >7.0, and A260/280 and 260/230 ratios >1.7. First strand cDNA was transcribed to the 5' end of capped RNAs, attached to CAGE “bar code” tags.