18S rRNA analysis of simulated oil spills
100 mL samples were collected at each time point from one of nine enclosures and filtered through a 0.22 um filter. The filter was flash frozen in liquid nitrogen and stored at -80C until processing. DNA was extracted using a modified version of the CTAB method and the V4-5 region of the 16S rRNA gene and V4 region of the 18S rRNA gene were amplified with fusion primers. Cleaned products were pooled and sequenced using 300 bp paired-end methods on an Illumina MiSeq.