Targeted-capture of Equus sp
Single stranded library preparation followed by in-solution hybridization capture using CNERs for Equine 23771 SNP panel. 250ng Library captured using 50ng CNERs in 100mM Tris pH8.0 with 10% DMSO and 200X blocker oligos for 65C/19.5hr. Sequenced in NextSeq for PE 2x300cycles.