GSM5210090: ML171549 3H Post Saline; Mus musculus; RNA-Seq
Quadriceps muscles were removed and weighed, flash frozen in liquid nitrogen, and RNA extracted using Trizol reagent. RNA was purified using Qiagen RNA columns and Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols on a Illumina HiSeq 2500