GSM5209209: DMD cardiac snRNA-seq; Mus musculus; RNA-Seq
For WT and DMD samples, we pooled two hearts of the same group for nucleus isolation. Additionally, we collected ventricular tissue from two cDMD mouse hearts that each was processed separately for nucleus isolation and sequencing. Cardiac nuclei isolation was performed as previously described Total cardiac nuclei were used to generate single nucleus RNA-seq libraries using Single Cell 3' Reagent Kits v3 (10xGenomics) according to the manufacturer's protocol