GSM5964829: IFNb rep3 (RNA-seq); Mus musculus; RNA-Seq
Cells were harvested after treatments and RNA was isolated using the Qiagen AllPrep DNA/RNA Mini Kit according to the manufacturer's guidelines. RNA-seq libraries were prepared with TruSeq Stranded mRNA LT sample preparation kit (Illumina) using Sciclone and Zephyr liquid handling robotics (PerkinElmer). Library amounts were quantified using Qubit 2.0 Fluorometric Quantitation system (Life Technologies) and the size distribution was assessed using Experion Automated Electrophoresis System (Bio-Rad). For sequencing, libraries were pooled, diluted and sequenced on an Illumina HiSeq 3000 instrument using 50 bp single-read chemistry. Library was performed at the Biomedical Sequencing Facility (https://www.biomedical-sequencing.org/) with PolyA capture to obtain mRNA molecules