GSM5709046: WT_d5,5; Mus musculus; RNA-Seq
Total RNA was extracted using Quick-RNA Mini Prep Plus kit (Zymo Research). For samples collected at 3.5 days in culture or later, neural progenitors were isolated using prominin-based MACs-sorting, and total RNA was extracted from prominin-positive isolated population. Illumina TruSeq Stranded mRNA poly-A selection