GSM5208893: 18571X2 RPM CGRP; Mus musculus; RNA-Seq
Enzymatic/mechanical digestion into single cell suspension then immediate sequencing 10X Chromium Single Cell Gene Expression Solution with 3' chemistry, version 3 (for RPMA and RPM samples). 10X Chromium Single Cell Gene Expression Solution with 3' chemistry, version 2 (for RPR2 sample). Targeted cell recovery of 8k cells (for RPM, RPR2, and RPMA_CMV samples) and 3.4k cells (for RPMA_CGRP). 10x gel beads and reverse transcription reagents were added to cell suspensions and loaded to chromium single cell controller to form gel-bead-in emulsions (GEMs). From GEMs, cDNA was generated from barcoded mRNA and subsequent A tailing, end repair, adaptor ligation, sample indexing was performed according to manufacturer. Libraries were assessed on Agilent D1000 ScreenTape on Agilent tech 2200 tapestation system and quantified KAPA Biosystems Library Quantification Kit for Illumina Platforms. Libraries were sequenced on the Novaseq 6000 w 2x150 paired end seq aiming for 200 million reads per sample.