GSM5708927: 143B-shFTO-IP; Homo sapiens; RIP-Seq
The extracted RNAs were separated by agarose gel electrophoresis and subjected to quality detection using a NanodropTM. Complete mRNAs were isolated with the Arraystar Seq-StarTM poly(A) mRNA Isolation Kit. The isolated mRNAs were chemically broken into fragments of approximately 100 nt. This process mainly included immunoprecipitation reactions, cleaning and elution. The m6A mRNA and input mRNA obtained after elution were enriched to construct an RNA sequencing library. The RNA sequencing library was generated using the KAPA Stranded mRNA-seq Kit (Illumina), and an Agilent 2100 BioAnalyzer was used to detect the quality of the library obtained. The library concentration was diluted to 8 pM and a cluster was generated in the Illumina cBot using the HiSeq 3000/4000 PE Cluster Kit (#PE-410-1001, Illumina). Finally, an Illumina HiSeq 4000 was used for sequencing (HiSeq 3000/4000 SBS Kit).