GSM5530735: C1qbp-KO-DNAme rep2; Mus musculus; Bisulfite-Seq
Genomic DNA was isolated using the QIAamp DNA mini kit (Qiagen). A total amount of 1.5 μg genomic DNA spiked with moderate lambda DNA were handled by MspI, followed by end repair and adenylation. Cytosine-methylated barcodes were ligated to DNA as per manufacturer's instructions. Then these DNA fragments were treated with bisulfite using EZ DNA Methylation-GoldTM Kit (Zymo Research).