Targeted-capture of Equus sp
Single stranded library preparation followed by in-solution hybridization capture using CNERs for Equine 23771 SNP panel. 300ng Library captured using 30ng CNERs in 100mM Tris pH8.0 with 10% DMSO, 3X Denhardt's and 200X blocker oligos for 65C/20.5hr. Sequenced in NextSeq for PE 2x150cycles.