RNA-seq of Phlegmariurus tetrastichus: sub-new growth leaves, replicate 3
Tissue samples were acquired from a single, mature Phlegmariurus tetrastichus specimen. Following tissue dissection, samples were immediately snap frozen in liquid nitrogen. Total RNA was extracted using the Spectrum Plant Total RNA Kit (MilliporeSigma). RNA-seq libraries for Illumina sequencing were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs).