GSM4794637: Brg1cKO_1; Mus musculus; RNA-Seq
For each samples, we used 3000 cells for total RNA preparation in replicates using NucleoSpin RNA XS kit (TakaraBio). mRNA were purified from total RNA using magnetic beads (New England Biolabs) Random priming strand-specific RNA-seq libraries were generated using with SMART-seq Stranded kit (TakaraBio) and single-end 75-bp sequencing was performed using Illumina HiSeq 2500 system.