18s amplification of salt marsh sediment: meiofauna extraction
Sediment samples collected from Barataria Bay, Louisiana, size selected <500 microns and >45 microns using sieves, meiofauna extracted using Ludox centrification, DNA extracted using DNeasy Powersoil kits, amplified using EMB 18s primers, libraries prepared using HiSeq Rapid SBS v2 kits, sequenced on Illumina HiSeq 2500