Illumina NovaSeq 6000 paired end sequencing; Multiome RNA+ATAC on human PBMCs (snATAC-seq data)
Multiome RNA+ATAC on human PBMCs (snATAC-seq data)
PBMCs from two donors were acquired from a LeukoLab (Clinical division of AllCells). Frozen PBMC samples were thawed quickly at 37 °C in a water bath. Two pools made for technical duplicates with ~500,000 cells for each donor per pool (50/50). Nuclei isolation, transposition, ATAC-seq and Gene Expression (GEX) sequencing libraries construction performed according to the manufacturer's Demonstrated protocol (CG000365 Rev A; 10X Genomics) and Next GEM Single Cell Multiome ATAC and Gene Expression user guide (CG000338 Rev A; 10X Genomics). Nuclei isolation, transposition, ATAC-seq and Gene Expression (GEX) sequencing libraries construction performed according to the manufacturer's Demonstrated protocol (CG000365 Rev A; 10X Genomics) and Next GEM Single Cell Multiome ATAC and Gene Expression user guide (CG000338 Rev A; 10X Genomics).