GSM5964847: LSC_48h_input; Mus musculus; ChIP-Seq
GMP, LSCs, and mAML cells were harvested, fixed, and sheared using an E220 focused-ultrasonicator (Covaris) After H3 antibody immobilization, sheared chromatin was indexed and pooled for IP against histone marks and transcription factors. ChIPed DNA was amplified, size-selected, and subject to sequencing on an Illumina NextSeq 500 or HiSeq2500 platform.