NOP106N18ITS_1
DNA was extracted with the MoBio PowerSoil DNA Isolation Kit (MoBio Laboratories, Inc., USA). To prepare the amplicon library, two PCR reactions were performed. For the first PCR reaction, the prokaryotic (bacterial and archaeal) 16S rRNA gene was amplified using the primer set 515F/806R (Caporaso et al. 2011), and the internal transcribed spacer region 1 (ITS1) of the fungal ribosomal operon was amplified using ITS1F/ITS2R (Bellemain et al. 2010). The second PCR reaction added barcodes to each sample and the Illumina sequencing adapters. After the second amplification, PCR products were quantified, then multiple samples were pooled together to form a 16S rRNA and an ITS1 amplicon library in equal proportions based on their molecular weights and DNA concentrations, respectively. The two amplicon libraries were purified and the average size and quantity of each library was assessed. Sequencing of each library was done on an Illumina MiSeq platform at Gnome Qubec Innovation Centre at McGill University (Montral, Canada).