GSM5901317: L_LSK; Mus musculus; RNA-Seq
LSK cells from the BM of LIP-subjected mice or unligated control mice were sorted by flow cytometry and subjected to single cell RNA-sequencing. The cell numbers were adjusted to 700–1200 cells per μl and added to 10x Chromium RT mix to achieve loading target numbers of around 20000 cells. cDNA synthesis was performed per the manufacturer's instructions (Chromium Single Cell 3' Reagent Kits User Guide (v3.1)), and library preparation and sequencing (Novaseq SP reagent kit; 100 cycles) were performed using the Novaseq 6000 platform (Illumina) per the manufacturer's instructions (Chromium Single Cell 3' Reagent Kits User Guide (v3.1)).