Illumina HiSeq 2000 paired end sequencing; DNA methylation sequencing of SLC22A18 between short stature patients and controls
DNA methylation sequencing of SLC22A18 between short stature patients and controls
Genomic DNA was extracted from whole blood using TIANamp Blood DNA Kit (Tiangen Biotech, Beijing, China), which was further treated with EpiTect Fast DNA Bisulfite Kit (QIAGEN, Hilden, Germany) for bisulfite conversion. The converted DNA was PCR-amplified with primer sequences designed to cover the CpGs in two promoter regions denoted as “Promoter 1” and “Promoter 2”  The PCR products were gel- and column-purified and used for DNA library preparation. The library was prepared with KAPA HTP Library Preparation Kit (KAPA Biosystems, Wilmington, MA) according to the manufacturer's protocol.