GSM5241422: Exp3_HSPB3_OE_Rep3; Homo sapiens; RNA-Seq
For gene expression analysis on LHCN-M2 cells, total RNA was isolated using Trizol (R2050; Zymo Research, USA), as per manufacturer's instructions. Followed by purification using RNA Clean & Concentrator (R1017; Zymo Research, USA) and DNase I Set (E1010; Zymo Research, USA), as per manufacturer's instructions. Library Preparation was done using TruSeq Stranded mRNA, according to manufactures protocol.