GSM5946844: uninfected_3; Mus musculus; RNA-Seq
Cells were harvested 20 hours post infection by a PBS wash followed by incubation of 10 min with ice-cold EDTA (5mM) and analyzed by flow cytometry (BD FACS Aria III) under continuous cooling at 4°C. Infected cells were sorted into 1.7ml low–bind tubes containing 25μl of lysis buffer (0.1% Triton in RNase-free water), placed immediately on dry ice and stored in minus 80. Single cells were processed for libraries generation according to the Cel-seq protocol (Hashimshony et al., 2016)