Whole genome bisulfite sequencing of adult diploid Crasstrea gigas ctenidia subjected to 24hrs of dessication at 27C.
Oysters were subjected to dessication conditions by removing them from water and incubating them in  room temperature (27C) air for 24hrs.Ctenidia tissue samples were collected and immediately stored at -80C. DNA was isolated using the E.Z.N.A Mollusc Kit according to the manufacturer's protocol.DNA was sent to ZymoResearch for whole genome bisulfite sequencing. Bisulfite treatment and library preparation were performed using Zymo-Seq WGBS Library Kit (Cat#: D5465).Paired end sequencing was performed on a NovaSeq (Illumina, Inc.) sequencer, with 150bp paired-end read lengths.