GSM6255070: B16-F10 control rep 1; Mus musculus; RNA-Seq
Clude RNA was harvested from tumor tissue using ISOGEN RNA isolation solution (Nippon gene). Then sequencing-grade RNA was purified using Rneasy mini plus kit (Qiagen). After polyA was enriched, 500 ng of mRNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina following manufacturer's protocols.