GSM5629063: SKOV3(CC)-WT_H3K27ac_input_rep2; Homo sapiens; ChIP-Seq
1 × 10e7cells were washed with PBS and fixed with 37 °C pre-heated 10% FBS MEDIA/1% formaldehyde at room temperature for 10 min, and quenched with 0.125 M glycine (V900144, Sigma) for 5 min, and sonicated to generate chromatin fragments of 200-600bp. The sheared chromatin was immunoprecipated with a specific antibody. Libraries were prepared according to Illumina's instructions accompanying the Paired-End DNA Samples Prep Kit. Briefly, DNA fragment end repair, add A base to 3'end, and connect sequencing adapter. PCR amplified with Illumina primers for 15 cycles and library fragments of ~250 bp were band isolated from an agarose gel. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Illumina HiSeq 2500 following the manufacturer's protocols.