GSM5964741: TKO_d0; Mus musculus; RNA-Seq
Total RNA was extracted using the RNeasy Mini Kit (Qiagen). After the QC process, mRNA was enriched using oligo(dT) beads. First, mRNA was randomly fragmented by adding fragmentation buffer, cDNA was synthesized using mRNA template and random hexamer primer, and custom double-strand synthesis buffer (Illumina), dNTPs, RNase H, DNA polymerase Synthesis was performed. Next, end repair, A-ligation, sequencing adapter ligation, size selection, and PCR enrichment were performed to complete the double-stranded cDNA library.