GSM5209377: ChIP-seq_cKO_H3K27ac; Mus musculus; ChIP-Seq
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP's DNA was subjected to end-repair and then was 3'adenylated. Adaptors were ligated to the ends of these 3'adenylated fragments. Next, polymerase chain reaction amplification was to amplify those fragments with adaptors from previous step.