GSM5135340: lung adenocarcinoma tissue-2-A; Homo sapiens; ncRNA-Seq
The library preparation processes included 3'-adapter ligation, 5'-adapter ligation, cDNA synthesis, PCR amplification and size selection of PCR amplified fragments of 134-160 base pairs in length (corresponding to ~14-40 nucleotide small RNAs). The sequencing library was quantified on an Agilent 2100 Bioanalyzer (Agilent Technologies, Inc.) using an Agilent DNA 1000 chip kit (Agilent Technologies, Inc.). Libraries were mixed in equal amounts and used for sequencing.