GSM5017762: Unstimulated, differentiated RXRab KO BMDMs rep3; Mus musculus; RNA-Seq
Total RNA was isolated with TRIZOL reagent according to the manufacturer's recommendation (Zymo Research, Direct-zol RNA MiniPrep Plus). cDNA library for RNA-seq was generated from 100ng total RNA using NEBNext Ultra II RNA Library Prep Kit for Illumina, according to manufacturer's protocol. Libraries were sequenced on the NextSeq 550, using the NextSeq500/550 High Output Kit v2.5 (75 Cycles). Three biological replicates were sequenced for each condition.