GSM5135116: C17_R2 RNA; Mus musculus; RNA-Seq
Total RNA was extracted from primary mouse bone marrow using the Ambion PureLink RNA Mini Kit (Thermo Fisher cat# 9655) with Trizol according to the maufacturer's protocol. Libraries were constructed from 5 ng total RNA using the Ovation RNA-Seq System V2 (Tecan). RNA-Seq libraries were prepared from 1 ug of cDNA using the TruSeq Stranded RNA Kit. Due to the amplification method, libraries are not stranded. Libraries were sequenced on the NovaSeq 6000.