PacBio CLR of Craspedostauros australis: strain CCMP3328
Long insert libraries have been prepared as recommended by Pacific Bioscienes according to the Guidelines for preparing size-selected 20 kb SMRTbellTM templates making use of the SMRTbell express Template kit. In summary, HMW gDNA has been sheared to 75 kb fragments with the MegaRuptorTM device (Diagenode) and 5,8 ug sheared gDNA have been used for library preparation. The PacBio SMRTbellTM library was size selected for fragments larger than 30 kb with the BluePippinTM device according to the manufacturers instructions.The size selected library has been loaded with 15 and 19 pM on plate on two Sequel SMRT cells (1M v3), Sequel polymerase 3.0 has been used in combination with the Sequel sequencing kit 3.0, run time was 10 hours for both SMRT cells.