RNAseq of human tumor: Sample 67TR
"Solid tumor total nucleic acid was extracted from FFPE tumor tissue sections and proteinase K digested. Chemagic 360 instrument using source-specific magnetic bead protocol was used to isolate total nucleic acid. Total nucleic acid was used for DNA library construction; RNA was purified after DNAseI digestion by magnetic bead purification. Nucleic acids were quantified by Quant-iT picogreen dsRNA reagent kit or Quant-iT Ribogreen RNA kit (life technologies).Quality was confirmed using a LabChip GX Touch HT Genomic DNA Reagent Kit or LabChip RNA High HT Pico Sensitivity Reagent Kit (PerkinElmer). Sequencing (WES and RNAseq) was performed on an illumina Hi-Seq 4000 system using patterned flow cell technology at TEMPUS, Inc. "