GSM5240735: 2hr_ATAC-seq_rep3; Homo sapiens; ATAC-seq
Cells were washed in cold PBS (containing IFNg for treated groups) and resuspended in lysis buffer (10mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl_2, 0.1% IGEPAL). The nuclei were pelleted and resuspended in 1x TD with 2.5ul TDE1 (Nextera DNA library prep kit, Illumina). These were incubated for 30min at 37oc. The tagmented DNA was purified using the MinElute PCR purification kit (Qiagen, 28004). Nextera DNA library prep kit and Nextera index kit (Illumina)