sRNA-seq of Gossypium arboreum ovule
Total RNA was isolated from the frozen 0, 5, 10, 15 and 20 dpa ovules with fibers using a modified protocol as described . Small RNAs of 15-30 nt in length were isolated on 15% PAGE with 7 M urea. After ligation to both 5 and 3 RNA adaptors, the purified RNAs were reverse transcribed using primers partially complementary to the adaptors. Subsequently, the small RNA libraries were sequenced on Illumina platform with SE50 mode.