GSM5596638: 3N; Homo sapiens; RNA-Seq
Fresh frozen HCC (hepatocellular carcinoma) samples with paired adjacent non-neoplastic tissue samples were used for total RNA extraction, which was performed using the Maxwell 16 LEV simplyRNA Tissue kit (Promega AS1280) following kit instructions and on the Maxwell 16 MDx automated instrument. RNA libraries were prepared from samples that met the TruSeq® Stranded Total RNA Sample Preparation Guide (15031048 E) input guidelines using the Illumina® TruSeq® Stranded Total (Gold) RNA Sample Preparation kit (Illumina Inc., San Diego, California, USA).