Targeted DNA-Seq of homo sapiens: bone marrow or peripheral blood from children with acute lymphoblastic leukemia
Firstly, genomic DNA was extracted from bone marrow and subjected to multiplex PCR. Then the products were combined and subjected to the first magnetic bead purification. Next, the linker sequence PCR and the second magnetic bead purification were carried out. Finally, DNA library quantification was determined by Qubit 3.60 Fluorometer.