Shotgun metagenomics of Autotrophic NRFeOx culture: long reads
Liquid cultures were filtered with 0.2 m pore-size fand subsequently placed in 2 mL centrifuge tubes and frozen. DNA was extracted using FastDNA SPIN Kit according to the manufacturers instructions. Library was prepared with1 g DNA using Ligation Sequencing Kit (ONT) and was sequenced with GridION.