GSM5484539: Pancreas-Input DNA; Mus musculus; ChIP-Seq
Lysates were clarified from sonicated pancreas and NFIC-DNA complexes were isolated with antibody. Libraries were prepared from purified DNA using "NEBNext Ultra II DNA Library Prep Kit for Illumina" from New England BioLabs (ref. E7645), as per the manufacturer's instructions. Briefly, DNA samples were processed through subsequent enzymatic treatments for fragmentation (5 min), end-repair, dA-tailing, and ligation to adapters Adapter-ligated libraries were amplified by limited-cycle PCR and extracted with a [single] double-sided SPRI size selection. Resulting average fragment size was 350 bp from which 120 bp correspond to adaptor sequences. Libraries were applied to an Illumina flow cell for cluster generation and sequenced on an Illumina instrument by following manufacturer's recommendations.