GSM4908764: 24hrs_F4; Gallus gallus; RNA-Seq
Total RNA was isolated from the retina using the Trizol/chloroform protocol. RNA quality and quantity was assessed on a 2100 Bioanalyser. All samples had an RNA Integrity Number (RIN) greater than 7.0. Libraries were prepared using the Ultra Directional RNA Library Kit for Illumina following the manufacturer's instructions. Libraries were validated on a 2100 Bioanalyser. Libraries were normalised to 4 ng/µL in 0.1x TE, pooled and sequenced at the Ramaciotti Centre for Genomics (UNSW, Sydney, Australia) on an Illumina NextSeq 500 platform (paired-end, 75 cycles).