PacBio long-read sequencing of Tamarix chinensis Lucheng1
Based on the high quality of gDNA, a single SMRT-bell library with a 40-kb long insert size was prepared using the SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences, Menlo Park, CA, USA) according to the manufacturers instructions. The library was sequenced on a PacBio Sequel II instrument (Pacific Biosciences, Menlo Park, CA, USA) with Chemistry 2.0 for 15 h per SMRT Cell 8M.