GSM5135380: IVF_ExE.2; Mus musculus; RNA-Seq
The prepared cells were transferred to lysis buffer by a mouth pipette. Reverse transcription was performed directly using Oligo (dT) primer in the lysate containg completely cytoplasm. The second-strand cDNAs were synthesised depending on the poly(A) tail attached to the 3′ end of the first-strand cDNAs. Fragment cDNA was prepared using Covaris sonicator (Covaris S220, Woburn, MA, USA) for sequence library construction. Kapa Hyper Prep Kit（KAPA , KK8504, Switzerland）was used to complete the sequence libraries.