RNA-seq of Tamarix chinensis
The 20 cm apical cuttings were collected from T. chinensis Lucheng Yihao on February 10, 2021. After washing with running tap water overnight, the cuttings were placed in 58  33  15 cm containers, with 20 cuttings per container, and maintained in hydroponic culture with half-strength Hoaglands solution. The cuttings were incubated in a growth chamber with a 16-/8-h (25/20C) day/night cycle and 65% relative humidity (Liu et al., 2022). The solution was refreshed every seven days. After two months of culture, the healthy cuttings with root length of 20-35 cm and shoot height of 18-20 cm were selected and subjected to salt stress. The cutting seedlings were divided into two groups, one maintained in the solution containing 300 mM NaCl and the other grown in the solution without NaCl treatment. Before imposing 300 mM NaCl, the cutting seedlings were pre-treated in the solution containing 200 mM NaCl for two hours to avoid salt shock (Shavrukov, 2013). After 7-day stress, the shoots (SL) and roots (SR) of both control and salt-treated plants were harvested, then the remaining plants were then transferred to the solution without NaCl for recovery. After a 35-day recovery, the shoots (RSCL) and roots (RSCR) of both control and salt-recovered plants were harvested, and the remaining plants were then re-transferred to the solution containing 300 mM NaCl. The shoots (RSSL) and roots (RSSR) of both control and salt-treated plants were harvested after a 7-day re-salt stress treatment. Each sample contained three independent biological replicates.