GSM5208143: Adipocyte 9; Homo sapiens; RNA-Seq
Aliquots of cultured preadipocytes and their respective in vitro differentiated adipocytes were used for RNA extraction using NucleoSpin® RNA (Macherey-Nagel). RNA integrity (RIN ≥ 9) was measured with Bioanalyzer 2100 (Agilent Technologies). mRNA was isolated from 1 µg RNA by poly-dT enrichment using the NEBNext Poly(A) mRNA Magnetic Isolation Module. After fragmentation, the samples were subjected to the workflow for strand-specific RNASeq library preparation (Ultra Directional RNA Library Prep II, NEB) and 75 bp single read sequencing was performed on Illumina NextSeq500.