GSM4838872: 621H::goxR-strep_ChAP; Gluconobacter oxydans; OTHER
Cell disruption, sonication of the DNA, purification of DNA bound to Strep-tagged GoxR, and the final DNA purification after digestion of protein was performed as described (PMID: 32453397). The obtained DNA fragments were prepared for sequencing using the NEBNext Ultra II DNA Library Prep Kit for Illumina following the manufacturer´s protocol for DNA fragments without size selection. Adapter-ligated DNA was amplified by using 15 PCR cycles. Quantification of the resulting libraries was performed with the KAPA library quantification kit and sequenced on a MiSeq (Illumina) using paired-end sequencing with a read-length of 2 × 150 bases.