GSM5135349: A253 EHF Expressing Rep1 mRNA; Homo sapiens; RNA-Seq
All cells were harvested in Trizol and total RNA was extracted using  Direct-Zol RNA mini-prep kit (zymo research) according to manufacturer's protocol. Illumina TruSeq RNA Sample Prep Kit was used with 1 ug of total RNA for the construction of sequencing libraries. Total RNA was enriched for mRNA before library construction.