GSM5208680: placenta_OBLA_331; Homo sapiens; miRNA-Seq
Whole placenta tissue was flash frozen in liquid nitrogen within 30 min of cesarean delivery. Total RNA was harvested using Trizol reagent. Total RNA was quantified using a NanoDrop Spectrophotometer ND-1000 (NanoDrop Technologies, Inc.) and its integrity was assessed using a 2100 Bioanalyzer (Agilent Technologies). Libraries were generated from 1000 ng of total RNA using the TruSeq® Small RNA Sample Prep Kit (Illumina), as per the manufacturer's recommendations. cDNA construct purification has been performed on a Pippin Prep instrument (SAGE Science). Final libraries were quantified using the Kapa Illumina GA with Revised Primers-SYBR Fast Universal kit (Kapa Biosystems). Average size fragment was determined using a LabChip GX (PerkinElmer) instrument. RNA libraries were prepared for sequencing using standard Illumina protocols