RNA-Seq of Aedes aegypti metagenome: adult female ovary pool
Pools were homogenized in TRIzol reagent and RNA was extracted following the manufacturers instructions, followed by treatment with TURBO DNase. RNA metagenomic libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina. Libraries were pair-end sequenced (2x150 bp) using the Illumina HiSeq 4000 platform at Novogene.