BGISEQ-500 paired end sequencing; scRNA-seq of murine mucosal associated invariant T (MAIT) cells after Fancisella tularensis infection
scRNA-seq of murine mucosal associated invariant T (MAIT) cells after Fancisella tularensis infection
Liver CD3+CD45+MR1-5-OP-RU tetramer+ MAIT cells were sorted by flow cytometry into a 10% FCS/PBS buffer. MAIT cells were sorted as single-cells using the Chromium 10X Genomics platform. Approximately 3,500 MAIT cells from naïve mice, or 10,000 at 13 dpi and 48 dpi were loaded per channel onto a Chromium controller (10x Genomics) for generation of gel bead-in-emulsions using 3′ gene expression kits (v3; 10x Genomics).  Sequencing libraries were prepared using Single Cell 3' Reagent Kits v3 (10x Genomics) and then converted using the the MGIEasy Universal Library Conversion Kit (BGI).