GSM5241862: Liver_NL_3; Mus musculus; RNA-Seq
Cells were trypsinized off culture plates, washed in PBS x2; normal lungs and livers were dissociated enzymatically into single cell suspensions, washed in PBS x2; KPC-bearing lungs and livers were dissociated enzymatically into single cell suspensions and enriched for CD45- populations, washed in PBS x2 Total RNA was extracted using Quick-RNA microprep kit, with DNAse I treatment. NEBNext Ultra II Directional RNA library prep kit was used to generate libraries. PolyRNA is first selected using oligodT beads. After cDNA synthesis, adapters are ligated for PCR enrichment.