GSM5241162: whole lung - Ctrl; Mus musculus; RNA-Seq
Neonatal lungs at PN14 were dissected and dissociated into single cell suspension by cold active protease (CAP). The single cell suspension prepared above was loaded onto a Chromium Single-Cell instrument (10x Genomics) to generate single-cell barcoded droplets (GEMs) as detailed in manufacture's protocol. The resulting libraries were pooled and sequenced across two lanes on an Illumina HiSeq2500 instrument in High-output mode by Novogene. Reads were aligned and subsequent analyses performed using the Cell Ranger (Pipeline).