Illumina HiSeq 2500 paired end sequencing; RNAseq evaluation of nicotinamide seed treatment analysed in Norway spruce seedlings
RNAseq evaluation of nicotinamide seed treatment analysed in Norway spruce seedlings
Seedlings were harvested after three months and snap frozen (-80 C nitrogen). Roots were grounded into a powder for RNA extraction.  Seeds were planted in wet peat and kept at 23 C in the dark until germination after one week. Growth conditions for the plants until harvest at three months:  23 C, 18/6 h light/dark, fluorescent lighting tubes (150 µmol m-2 s-1). Treated samples: Norway spruce seeds, collected in the orchard of Öhn (origin 57° 00´ N, alt. 55 m), were treated in a water solution of 2.5 mM NIC and 1 µM Tween 80 for 4 h with gentle shaking at 23 C in the dark.  Total RNA was extracted using Qiagen RNeasy Plant Mini Kit  Illumina TruSeq Stranded mRNA Library Preparation Kit