Ion Torrent PGM sequencing of SAMD00286518
The 16S rRNA genes were amplified using KOD DNA polymerase and the primers: 8F (AGA GTT TGA TYM TGG CTC AG) with adaptor and tag sequences and 365R (TGC TGC CTC CCG TAG GAG T) with adaptor sequence. Sequencing was performed using an Ion PGM Hi-Q View sequencing kit. Raw sequence reads were quality-filtered and assigned to each sample.