GSM5595978: CE4; Mus musculus; RNA-Seq
mice adipose tissue were miced and homogenized in dounce homogenizer on ice. The homogenized adipose tissue nuclei were washed with 1% BSA in PBS and filtered 35 um straners. Nuclei suspensions were stained with Hoechst 33342 and loaded to FACS sorting and sorted based on RFP fluorecence Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry)