GSM5708860: RNA-Seq PWK mEpiSCs BioRep698; Mus musculus; RNA-Seq
mEpiSCs were lifted with collagenase (to avoid feeder contamination) and resuspended to single cells with accutase. RNA was extracted using trizol. After RiboGreen quantification and quality control by Agilent BioAnalyzer, 129-500ng of total RNA with RIN values of 9.5-10 underwent polyA selection and TruSeq library preparation according to instructions provided by Illumina (TruSeq Stranded mRNA LT Ki