GSM4907359: BAT SVF 8w C57BL/6J WT; Mus musculus; RNA-Seq
Brown adipose tissues were isolated from interscapular area of three 8 week old, male WT C57BL/6 mice. BAT was minced and digested with 1mg/ml type-A collagenase (Gibco) in DMEM (Gibco) for 30 minutes at 37°C. The SVF was obtained by centrifugation and live cells were sorted using flow cytometry following dead cell exclusion using 7-AAD. Single cell libraries were generated using the ChromiumTM Single cell 3'library and gel bead kit v2 (PN #120237) from 10x Genomics.