GSM5059634: M6; Drosophila melanogaster; RNA-Seq
Third instar larval central nervous systems were dissected out and other tissues removed. Larval carcasses were prepared by removing all internal tissues, leaving only muscle and cuticle. Total RNA was extracted using Trizol. Libraries were constructed using the Illumina TruSeq RNA sample Prep Kit v2 (Cat #RS-122-2002), using 500 ng of total RNA as input.