GSM5709918: CBTTC_6781; Homo sapiens; RNA-Seq
CSF (0.2 mL) was mixed with 1 ml of QIAzol (Qiagen, Hilden, Germany) and incubated for 5 min at room temperature. Next, 0.4 ml of chloroform was added and mixed. The aqueous phase was obtained by centrifugation at 14,000 x g for 15 min at 4°C, and RNAs were isolated using the miRNeasy Mini kit (Qiagen) according to the manufacturer's protocol. To perform library generation with the NuGen Ovation Solo system, the purified RNAs were concentrated using a RNA Clean & Concentrator (Zymo Research Corp), and libraries were prepared according to the manufacturer's instructions. The quantification of libraries was estimated using a KAPA library quantification kit (Roche Sequencing and Life Science, Wilmington, MA).