GSM5210264: Dp16/D-KO_F2; Mus musculus; RNA-Seq
The hippocampi were isolated from the brain on an ice-cold platform and fast frozen in liquid nitrogen. Total RNA was isolated using the TRIzolTM reagents. The quantity and quality of the RNA were determined by using Agilent 2100 bioanalyzer One μg RNA from each sample was used for library construction. Briefly, mRNA was first enriched with Oligo(dT)-coupled beads and then fragmented. Random N6 primers were used to synthesis double strand cDNAs. Bubble adaptors were ligated onto the products before PCR amplification. The PCR products were denatured into single strands by heating and the circularized with splint oligos before subjected to sequencing