GSM6255501: liver cancer, response4752; Homo sapiens; RNA-Seq
Total RNA of cell was extracted by using Trizol. 1.0 ug of total RNA was used for the construction of sequencing libraries. Library preparation were performed by Epibiotek (Guangzhou, China). Briefly, total RNA were treated to remove ribosomal RNA by using GeneRead™ rRNA Depletion Kit（Qiagen, Hilden, Germany, Cat No. 180211）. Ribosome depleted RNA was fragmented and then used for constructing strand-specific RNA libraries by using VAHTS Stranded RNA-seq Library Prep Kit for Illumina (Vazyme, Nanjing, China, Cat. No NR602) accoding to manufacturer instructions.