Capture sequencing of Treponema pallidum subspecies pallidum
Libraries were constructed from genomic DNA using the KAPA HyperPrep kit and cleaned with 0.7x AMPure XP beads. The libraries were then enriched for Treponema pallidum subspecies pallidum sequences using a custom myBaits (Arbor Biosciences) oligonucleotide probe set designed against three T. pallidum reference genomes. The resulting libraries were then sequenced on a 2x300bp MiSeq run.