Tissue-based RNA-Seq of Lysmata wurdemanni: thoracic ganglion  and cerebral ganglion, adult
Approximately 10 g of total RNA was employed for Poly(A) mRNA isolation using oligo-dT magnetic beads (Invitrogen). Subsequently, the mRNA was fragmented into small pieces in the presence of divalent cations (fragmentation buffer (Ambion, #AM8740)) at 94C for 5 minutes using an ultrasonicator. The RNA fragments were reverse-transcribed into the cDNA library by using the mRNA-Seq preparation kit (Illumina, San Diego, USA)