GSM5134406: TCE_NAC_Male_Rat30_AS; Rattus norvegicus; RNA-Seq
RNA was extracted from placental tissue using the RNeasy Plus Mini Kit (Qiagen) following the manufacturer's recommended protocol with a modification. A FastPrep-24 tissue lyser (MP Biomedicals; Solon, OH, USA) was utilized prior to the gDNA elimination step to achieve maximum quality tissue homogenization. DNA was extracted from placental using the NucleoSpin Tissue kit (Machery-Nagel; Düren, Germany) according to the manufacture's recommended protocol. RNA and DNA concentrations were determined using a NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific; Waltham, MA, USA). Isolated nucleic acids were subsequently stored at -80°C until further processing. RNA libraries were prepared for sequencing using standard Illumina protocols