GSM5133966: CPC_FLKO_5; Mus musculus; RNA-Seq
RNA was isolated from a total of three sorts of SP-CPCs using the NucleoSpin RNA XS kit (Macherey Nagel). Each sort consisted of the cardiomyocyte- and erythrocyte-depleted cardiac cell suspension from 4 wt and 4 flt3L-/- mice. RNA quality was controlled on an Agilent 2100 Bioanalyzer using RNA Pico CHIP (Agilent Technologies). RNA-seq library preparation and sequencing were performed at the Quantitative Genomics Facility of the D-BSSE of the ETHZ (Basel, Switzerland). RNA-seq library quality was assessed with a Fragment Analyzer (Advanced Analytical). Unstranded RNA-seq libraries were produced using the low-input SMART-Seq v4 kit (Clontech/Takara).