GSM4907950: Donor3-Hyp-sEVs_rep1; Homo sapiens; miRNA-Seq
mEVs and sEVs isolated from MSC were suspended in 700 µL of Qiazol reagent (Qiagen,). EV RNA was extracted with the miRNeasy Micro Kit (Qiagen) according to the manufacturer's instructions. miRNA content was assessed by performing the Qubit microRNA Assay Kit and RNA quality and sizes were checked with Agilent 2100 Bioanalyzer using the Agilent Small RNA chip according to manufacturer's protocol. Each sequencing library was constructed from 9.2ng of isolated miRNA. Small RNA libraries were prepared and amplified using QIAseq miRNA Library kit (Qiagen), following manufacturer's instructions. Libraries were pooled after quality check and quantification by TapeStation (Agilent Technologies) using Agilent High Sensitivity D1000 ScreenTape. Pooled libraries were quantified by real-time qPCR following “Sequencing Library qPCR Quantification” Guide (Illumina Inc.) and sequenced by Illumina NextSeq platform using the 75 cycles high output kit (Illumina Inc.).