GSM5484338: Synovial tissue - none; Homo sapiens; RNA-Seq
Synovial biopsies were mechanically minced and enzymatically digested with 100 ug/ml Liberase TL and 100 ug/ml DNAseI in pre-warmed RPMI 1640 containing 2 mM glutamine and 25 mM HEPES  for 30 min at 37°C. The digestion was stopped by adding 20 v/v % with RPMI containing 20% FCS and the dissociated tissue was filtered through a 40 um cell strainer. Red blood cells were lysed with RBC lysis buffer. Cell viability was analysed with a LUNA-FL dual fluorescence cell counter. cDNA libraries were prepared for sequencing following the 10x Genomics protocol (Chromium Single Cell 3ʹ Reagent Kits (v3 chemistry)