GSM4907469: Dfl1_WGBS; Mus musculus; Bisulfite-Seq
Briefly, 1 ug of genomic DNA was spiked-in with unmethylated lambda-phage DNA (1:200) and sonicated using Bioruptor (9 cycles 30 seconds on, 30 seconds off). The sonicated DNA fragments were end repaired, A-tailed and ligated with methylated adaptors (all using NEB kits). The adaptor ligated fragments were then treated with sodium bisulfite using the Methylcode kit (Invitrogen) for a total of 4 hours according to manufacturer's protocol. The bisulfite converted, adaptor ligated fragments were then amplified with Kapa Hifi Hotstart Uracil+ PCR mix with NEB universal dual indexing primers. The libraries were then sequenced on an Illumina NovaSeq (150 bp paired-end reads) Whole genome bisulfite sequencing