GSM5208172: Piglet_restricted_metformin_sample26; Sus scrofa; Bisulfite-Seq
High quality genomic DNA (400ng) was isolated from adipose tissue samples with Gentra PureGene tissue kit (Qiagen) according to manufacturer's instructions. DNA was bisulfited with the EZ DNA Methylation-Gold Kit (Zymo Research), according to manufacturer's instructions. Bisulfite conversion rate was over 99%. Libraries were were prepared for sequencing using standard Illumina protocols and Sequencing was performed on a HiSeq 3000 (Illumina).