GSM5240133: Sample_MEL_3xKO_Ctrl_dox_rep2; Homo sapiens; ATAC-seq
50,000 freshly harvested cells were lysed in 50 μl ATAC lysis buffer (Tris 10 mM pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% NP-40) and incubated with Illumina Tn5 transposase at 37°C for 30 min. Samples were purified using Agencourt AMPure XP beads. Barcoding and library generation were performed using the NEBNext Q5 Hot Start HiFi PCR Master Mix with PCR amplification for 12 cycles.