GSM5016615: naive CD8 T cells, Tcf1 ChIPseq; Mus musculus; ChIP-Seq
Naïve CD8+ T cells were sort-purified from the spleen, crosslinked and immunoprecipitated with anti-Tcf1 antiserum The DNA segments from ChIP were end-repaired and ligated to indexed Illumina adaptors, followed by amplification by PCR with a low number of cells (18-21 cycles). The resulting libraries were seqeunced with Illumina Hiseq-2000 platform.