Genetic basis for color plasticity in Rapana Venosa based on microsatellite, DNA methylation and transcriptome
Total RNA was extracted separately from the tissues of each sample by the CTAB method, whose quality was checked by agarose gel electrophoresis (AGE) and a Nanodrop 2000 instrument. The mRNA was then fragmented and used as a template for synthesizing the first cDNA chain. By RNase H and DNA polymerase I, the double-stranded cDNAs were performed with the fragmented mRNA as templates. The double-stranded cDNA was purified by AMPure XP beads and then ligated with end repair adapters using T4 DNA ligase (Invitrogen, Carlsbad, CA, USA). Finally, the cDNA library was generated with PCR enrichments, and was then sequenced using Illumina HiSeq 4000 after being qualified