GSM5484678: PBS_M5; Mus musculus; RNA-Seq
Tumours were surgically excised 20-days after implantation, and then snap-frozen using liquid nitrogen. Total RNA was then extracted by mechanical disruption of the tissue and using Qiagen RNeasy Mini kit reagents (Cat#74004) including on-column DNase digestion. The mRNA fraction was selected from the isolated total RNA by poly-A enrichment before being converted to cDNA for the construction of sequencing libraries. The cDNA was end-repaired, A-tailed and adapter-ligated. RNA-seq libraries were prepared for sequencing using standard Illumina protocols.