GSM5989877: Wildtype Ipsilateral [3545 IL WT]; Mus musculus; RNA-Seq
Single cell suspension of microglia/myeloid cells from the ipsilateral and contralateral hemispheres from wild-type and Nhe1-KO brains after 3-days of traumatic brain injury were prepared using Neural Tissue Dissociation Kit (Miltenyi Biotec, Germany) following manufacturers protocol. CD11b+ microglia/myeloid cells were sorted by MACS. Total RNA was extracted using Direct-zol RNA MicroPrep kit (Zymo Research). Library was prepared using Smart Seq v4 library preparation kit according to manufacturers instructions