5'P-RNA: GsSir2/Ago bound RNA with 5'P
pBADHisA plasmid with the cloned protein GsSir2/Ago from Geobacter sulfurreducens PCA (NP_952414.1) expressed in E.coli DH10B cells together with pCDF-Kn plasmid. GsSir2/Ago with bound RNA was purified as indicated in Zaremba et al., 2021 (doi: https://doi.org/10.1101/2021.12.14.472599) using gentle chemical reagent for cell disruption and buffer solutions with 100mM NaCl. GsSir2/Ago bound RNA was purified using phenol isolation. Then RNA sample was converted to DNA library using Small RNA-Seq Library Prep Kit (Lexogen cat#052).