RNA-seq of equine day 11 conceptus
RNA-Seq library preparation was performed starting from 200 ng total RNA by using the SEQuoia Complete Stranded RNA Library Prep Kit (Bio-Rad Laboratories, Inc.; Switzerland) which permits the capture of long as well as short RNAs in a single library. Two pools of 11 samples each were prepared, and sequencing was performed on one SP flow cell on an Illumina NovaSeq 6000 instrument (Functional Genomic Center Zurich). Paired-end sequencing was performed with 92 bp for read one (cDNA insert) and 8 bp for read 2 (UMI sequence for removal of PCR duplicates).