GSM5988532: D19_F; Homo sapiens; RNA-Seq
Tissues were first cryomilled. Total RNA was harvested with Trizol and then purified with Direct-zol RNA Miniprep (R2052). The total RNA was poly(A)-selected with NEBNext Poly(A) mRNA magnetic isolation module (NEB #E7490) and the library was constructed with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina