GSM5708524: 81E2; Mus musculus; RNA-Seq
Muscle were digested, single cell suspension stained and eosinophils were sorted as follow: CD45+ NK1/Ly6G/CD3e- CD11bing SiglecF+, diretly into RNAzol Sample quality control was performed using the Agilent 2100 Bioanalyzer. Qualifying samples were then prepped following the standard protocol for the NEBnext Ultra ii Stranded mRNA (New England Biolabs). Sequencing was performed on the Illumina NextSeq 500 with Paired End 42bp × 42bp reads.