RNAseq of Gryllodes sigillatus: pooled adult female gut samples
RNA was extracted using a Trizol-Chloroform extraction followed by a Purelink RNA mini kit with a DNase step. RNA extracted from 9 specimens was pooled together before sequencing to get a total of 40 samples. Sample prep and sequencing and read demultiplexing were performed by Exeter Sequencing service at the University of Exeter (UK). Paired-end 100 bp reads were obtained by multiplexing the samples on four lanes of an Illumina HiSeq 2500. 10 pools were merged on a lane.