GSM5964727: Control FGSCs rep1; Mus musculus; RNA-Seq
Total RNA of FGSCs was extracted using Trizol reagent (Life Technologies, CA, USA), in accordance with the manufacturer's instructions. The quality of total RNA was analyzed using Nanodrop Lite (Thermo Fisher Scientific ). A total of 1 mg of RNA was reverse-transcribed into cDNA using a reverse-transcription kit (Takara, Tokyo, Japan). RNA libraries were prepared for sequencing using standard Illumina protocols