GSM5135404: siKmt2e_ExE.2; Mus musculus; RNA-Seq
The prepared cells were transferred to lysis buffer by a mouth pipette. Reverse transcription was performed directly using Oligo (dT) primer in the lysate containg completely cytoplasm. The second-strand cDNAs were synthesised depending on the poly(A) tail attached to template switching by TSO. Fragment cDNA was prepared using Covaris sonicator (Covaris S220, Woburn, MA, USA) for sequence library construction. Kapa Hyper Prep Kit（KAPA , KK8504, Switzerland）was used to complete the sequence libraries.