Metagenomic illumina sequencing of Ferrovum dominated stalactite biofilm from acid mine drainage
Total genomic DNA was isolated from stalactite biofilm using DNeasy PowerLyzer PowerSoil kit (Quiagen, Germany). The Illumina sequencing libraries were prepared using TruSeq Nano DNA high-throughput kit. The fragmentation of DNA was performed on Bioruptor Plus sonication device. The libraries were sequenced using the Illumina HiSeq 2500 RR 2x250 bp technology.