genome survey of  Mesona:  leaf
A total amount of 5 ng gDNA per sample was broken to 270 bp by physical fragmentation (ultrasonic vibration), and then the small fragment sequencing library was constructed by terminal repair, adding connector, target fragment selection and PCR sequentially. The fragment size and quantity of the library was detected by 2100 and Q-PCR, respectively. After then, the library was fixed to the sequencing chip by bridge PCR and then sequenced by Hiseq sequencer.