GSM5241366: NEU_WT_INF_01; Mus musculus; RNA-Seq
Two independent experiments were performed. For each experiment 14 to 15 tails (i.e. infected tissue) per group were collected, pooled and digested at two weeks post infection. Cell surface markers were stained with antibodies. Live infected neutrophils (CD45+Ly6G+CD11b+Wasabi+) or live uninfected neutrophils (CD45+Ly6G+CD11b+Wasabi-) were sorted by FACS in PBS supplemented with 1% BSA using a FACS ARIA Fusion (BD Biosciences). Single cell RNA-sequencing libraries were prepared according to the manufacturer's instructions using Chromium Single Cell 3′ Library & Gel Bead Kit v3 (10x Genomics, PN-1000092) and Chromium Chip B Single Cell Kit (PN-1000074) with the Chromium Controller & Next GEM Accessory Kit (10x Genomics, PN-120223). Library quality control and quantification were performed using a KAPA Library Quantification Kit for Illumina Platforms (Kapa Biosystems, KK4873) and the 2100 Bioanalyzer equipped with a High Sensitivity DNA kit (Agilent, 5067-4626).