GSM4838148: WT-MeOH-2; Methylorubrum extorquens PA1; RNA-Seq
The total RNA was extracted with the RNeasy Plus Mini Kit (Qiagen); rRNA was depleted with the Ribo-Zero rRNA removal kit (Illumina); the quality of resulting RNA samples was determined with an Agilent 2100 BioAnalyzer and Qubit assay. RNAs were fragmented for 15 minutes at 94 °C. First strand and second strand cDNA were subsequently synthesized. cDNA fragments were end repaired and adenylated at 3'ends, and universal adapter was ligated to cDNA fragments, followed by index addition and library enrichment with limited cycle PCR.