Cladosporium velox genome sequencing
Purified DNA sample,  is sheared into smaller fragments with a desired size by Covaris S/E210 or Bioruptor firstly.    Then the overhangs resulting from fragmentation are converted into blunt ends by using T4 DNA polymerase, Klenow Fragment and T4 Polynucleotide Kinase.    After adding an A base to the 3' end of the blunt phosphorylated DNA fragments, adapters are ligated to the ends of the DNA fragments.    The desired fragments can be purified though gel-electrophoresis, then selectively enriched and amplified by PCR.    The index tag could be introduced into the adapter at the PCR stage as appropriate and we did a library quality test.    At last, the qualified BS library would be used for sequencing