NextSeq 500 paired end sequencing; Single nuclei chromatin profiles of midbrain from genetically distinct mouse strains reveal cell identity transcription factors and cell type-specific gene regulatory variation
Single nuclei chromatin profiles of midbrain from genetically distinct mouse strains reveal cell identity transcription factors and cell type-specific gene regulatory variation
The single nucleus ATAC-seq was performed according to Chrominum Single Cell ATAC Reagent Kits User Guide (CG000168 RevB) with Chromium Single Cell E Chip Kit (10X, 100086 In brief, nuclei suspension was loaded with a targeted recovery rate of 10 000 nuclei per sample. Chromium Single Cell ATAC Library & Gel Bead Kit (10X, 1000111), Chromium Single Cell ATAC Gel Bead Kit (10X, 1000085), Chromium Single Cell ATAC Library Kit (10X, v1, 100087), Chromium i7 Multiplex Kit N Set (1000084), Dynabeads MyOne Silane (2000048)