Draft genome sequence of a plant growth-promoting bacterium Pseudomonas pseudoalcaligenes KB-10
The sequencing libraries were preparedaccording to the manufacturers instructions of TruSeq DNA Nano Sample Preparation Kit(Illumina,Inc., San Diego, CA, USA). Briefly, Fragmentation of 100ng of genomic DNA was performed usingadaptive focused acoustic technology.(AFA; Covaris) and the fragmented DNA is end-repaired tocreate 5-phosphorylated, blunt-ended dsDNA molecules. Following end-repair, DNA was sizeselected with bead-based method. These DNA fragments go through the addition of a single A base,and ligation of the truseq indexing adapters. The purified libraries were quantified using qPCRaccording to the qPCR Quantification Protocol Guide (KAPA Library Quantificatoin kits for IlluminaSequecing platforms) and and qualified using the Agilent Technologies 2200 TapeStation. (Agilenttechnologies). And then the paired-end (2100 bp) sequencing was performed usingthe HiSeq4000 platform (Illumina, San Diego, USA).