GSM5708623: High CIN - CINhigh_WT2; Mus musculus; RNA-Seq
Primary tumors were resected under sterile conditions 14 days after orthotopic implantation in the mammary fat pad of BALB/c immune competent mice. The entire tumor was immediately place in RPMI media (Corning) on ice and dissociated using both mechanical and enzymatic digestion (Mouse Tumor Dissociation Kit #130-096-730 , Miltenyi Biotec) generally within 1 hour of surgical resection 10X Genomics, Single Cell 3′ V2 Reagent Kits User Guide PN-120233