GSM4908656: mouse AML cells without chemotherapy mouse 4; Mus musculus; RNA-Seq
Mouse AML cells were sorted and collected from harvested mouse bone marrow with cell sorter based on expression of CD45.1 and iRFP. The sorted cells were flash frozen on dry ice, and RNA was harvested using Trizol reagent and Qiagen DNA/RNA microkit. Illumina TruSeq RNA Sample Prep Kit was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols