GSM5016411: apCAFs2; Mus musculus; RNA-Seq
ApCAFs and nonapCAFs from tumor-bearing mice were FACS sorted as FSC-AhighCD45-CD31-EPCAM-mCherry-PDPL+PDGFRa+MHCII+ and FSC-AhighCD45-CD31-EPCAM-mCherry-PDPL+PDGFRa+MHCII- cells respectively. Total RNA was isolated by using Single Cell RNA Purification Kit (Norgen Biotek) according to manufacturer's instructions. RNA libraries were prepared for sequencing using NEB Next Ultra RNA Library Prep Kit.