NextSeq 500 paired end sequencing; RNA-seq of mouse common lymphoid progenitors, innate lymphoid cell progenitors and NK cell progenitors
RNA-seq of mouse common lymphoid progenitors, innate lymphoid cell progenitors and NK cell progenitors
CLP, ILCP and NKP from Vav1+iCre FOXO1,3flox/flox mice (C57BL/6 background) mice were FACS sorted. 200-400 cells were used for subsequent processing.  Cells were treated with the single cell lysis kit from Thermo Fisher (cat# 4458235) including DNAse treatment. The reaction containing the stop solution was used directly in the next step for nucleic acid library construction. The library preparation was carried out using the SMART-Seq Stranded Kit (Takara) according to the manufacturer's instruction. The quality of every cDNA library was determined on an Agilent Bioanalyzer instrument according to the manufacturer's protocol. All libraries were quantified with the KAPA-SYBR FAST qPCR kit (Roche).