GSM5989913: 20065FL-02-02-12; Sus scrofa; ATAC-seq
Up to twenty minutes letter, liver tissue was sampled  in media tissue-specific for liver tissue (William's E medium with 1x hepatocyte plating/thawing medium A; Thermofisher). Then samples were transported in +4C to the laboratory. Then they were centrifuged and undergone 10-minutes digestion in 37°C using 1xAcutase (Stempro). After that, they will be filtered by using a 70um cell strainer (Sigma), washed with 10% FCS DMEM/F12 (Gibco) and then slowly frozen in cryotubes with cryoprotectant Cryostor 10 (Stemcell) and Mr Frosty™ Freezing Container to the temperature -80°C. ATAC libraries were prepared using TruePrepTM DNA Library Prep Kit V2 for Illumina® (Vazyme) according to attached protocol.