Genome
DNA with a center of mass over 100 Kb was isolated using the Nucleobond Kit as per manufacturers protocol (Macherey-Nagel, Bethlehem, PA). Approximately 20 ug DNA was sheared to a center of mass of 10-20 Kb using a Megaruptor 3 system (Diagenode, Denville, NJ). HiFi sequencing library were constructed following the HiFi SMRTbell protocols for the Express Template Prep Kit 2.0 as per manufacturers recommendations (Pacific Biosciences, California). The library was sequenced using Sequel binding and sequencing chemistry v2.0 in CCS mode in a Sequel II system according to manufacturers guidelines with movie collection time of 30hrs