18S amplicons of soil microbes, McMurdo Dry Valleys
The 18S rRNA gene was amplified using Earth Microbiome Project primers, 1391f-EukBr, V9 region. Unique barcodes corresponding to unique samples were affixed. Then amplicons were normalized and pooled into a library which was sequenced on a 2x150 Illumina MiSeq. Sequences were demultiplexed by barcode into individual files for each unique sample.