16s rRNA  amplicon  sequence of permafrost soil
gDNA was extracted from soil sample using MP Bio kit then cleaning and concentration of gDNA was done followed by PCR amplification by universal primer for 16s v3-v4 region. Quality and quantity assessment by Qubit Flurometer and Agarose Gel Electrophoresis. Multiplex amplicon lib were prepared on Illumina MiSeq and raw reads were obtained.