GSM4794772: ES; Homo sapiens; ATAC-seq
CBMCs were separated using an equal proportion of Ficoll-Paque Plus. Nuclei suspensions were obtained by adding lysis buffer (10 mM Tris-HCl, 3 mM MgCl2, 10 mM NaCl , 0.1% Tween-20, 0.1% Nonidet P40 Substitute, 1% BSA). The final libraries were constructed via PCR with P5 and P7 primers in Illumina® bridge amplification. scATAC-seq libraries were generated according to the Chromium Single Cell ATAC protocol (10x GENOMICS)