GSM5240823: Mixture of blood samples from three dairy cows with 7 dpi; Bos taurus; miRNA-Seq
TRIzoL kit (Invitrogen, Carlsbad, CA, USA) was used to extract total RNA from blood at different induction times (0, 1, 3, 5, and 7 days) according to the manufacturer's instructions. A total of 1.5 μg of qualified RNA was taken, and EBNext®Ultra™ sRNA sample pre kit was used to construct the miRNA library according to the product instructions.