GSM4908553: shDKC1 total RNA replicate 2; Homo sapiens; RNA-Seq
Polysomal separation was performed as follows: samples were loaded in 10-50% linear sucrose gradients, ultra-centrifuged and fractionated with an automated fraction collector. All the fractions containing subpolysomal or polysomal RNA were identified and pooled in two separate tubes. RNA was purified by extraction with 1 volume of phenol-chloroform and precipitated with isopropanol. RNA libraries were prepared for sequencing using standard Illumina protocols: Illumina TruSeq RNA Sample Prep Kit was used with 1.5 ug of total RNA as input.