Day 4 differentiated myocytes from Immortalized DEL2 mutant Rep 2: Sample Day_4_DEL2_Rep2
Total RNA was collected using the RNEasy kit (74106, Qiagen). RNA was converted to cDNA using the Smart-Seq2 protocol. Libraries were constructed with the Nextera DNA Flex Library Prep Kit (Illumina). Libraries were sequenced on the Illumina NextSeq500 with paired-end 43 bp reads to a depth of 5 to 40 million reads.