GSM5596071: Y10 rep1; Chromochloris zofingiensis; RNA-Seq
RNA was extracted from 100 mL of algal culture obtained from the control (without LEV treatment) and treatment (10 mg/L LEV treatment) under heterotrophic condition at mid-phase (15 days). RNA quality and quantity were determined by a NanoDrop 2000 sepectrophotometer (Thermo Scientific) and an Agilent 2100 RNA 6000 Nano kit, respectively. cDNA libraries were constructed form 1 μg total RNA via a NEBNext®Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) according to the manufacturer' instructions. Afterwards, the clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumia)