GSM5602219: small EV from Ctrl-treated cortical neurons 8 8CExo; Mus musculus; ncRNA-Seq
After washing with cold PBS, cells and EVs were lysed directly in homogenization buffer provided by RNeasy or miRNeasy RNA isolation kits (Qiagen) smallRNA sequencing: control- or BDNF-treated cortical neurons and corresponding sEVs were prepared using NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs) as per manufacturer's instructions and sequenced in Illumina HiSeq2000. 150 ng of RNA was used as input material. RNA sequencing: Total RNA was used to prepare the sequencing library according to the manufacturer's protocol using the TrueSeq RNA library prep kit v2 (Illumina, USA). As a starting material, 500ng RNA was used. Multiplexed libraries were directly loaded onto an Ilumina Hiseq2000 using a 50 bp single read setup. smallRNA sequencing and RNA sequencing