GSM5135096: K562 MINT-Seq reanalysis rep1; Homo sapiens; RNA-Seq
RNA was extracted using TRIzol (Life Technologies), 4sU-labeled RNA was exctracted according to Duffy et al., Mol Cell, 2015.  M6A methylated RNA was immunoprecipitated by anti-m6A antibodies (202003, SYSY). RNA libraries were prepared using NEB Next Ultra II RNA library prep kit for illumina according to manufactorer's instructions RNA was extracted using TRIzol (Life Technologies), 4sU-labeled RNA was exctracted according to Duffy et al., Mol Cell, 2015.  M6A methylated RNA was immunoprecipitated by anti-m6A antibodies (202003, SYSY). RNA libraries were prepared using NEB Next Ultra II RNA library prep kit for illumina according to manufactorer's instructions