GSM5901022: CT26_PD1_40; Mus musculus; RNA-Seq
At end-stage, tumor-bearing mice were sacrificed by cervical dislocation and RNA was isolated from tumor tissue using TRIzol (Life Technologies). Starting from total RNA, poly-adenylated fragments were isolated, reverse transcribed and converted into indexed sequencing libraries using the Illumina TruSeq RNA sample preparation kit V2 according to the manufacturer's instructions. RNA libraries were prepared for sequencing using standard Illumina protocols.