Illumina MiSeq sequencing; MAGE-seq E. coli primary sigma factor RpoD amplicon high-throughput genotyping
MAGE-seq E. coli primary sigma factor RpoD amplicon high-throughput genotyping
Timepoint samples collected from a pooled competition experiment carried out in a turbidostat. Exponential growth in a turbidostat that maintained cell density between OD600 0.2 and 0.4 Genomic DNA prepped using the Illustra Bacterial Genomic DNA prep kit PCR amplicon of the E. coli rpoD genomic loci. Additional PCR to add illumina adapters.