liver 10
For RNA library preparation, the RNA of each tilapia individual from each tilapia line was diluted to the same concentration with RNase-free water and then pooled in equal amounts to obtain three groups. A total of 1 g RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA) following manufacturers recommendations and index codes were added to attribute sequences to each sample. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v4-cBot-HS (Illumina, San Diego, CA, USA). After cluster generation, the library preparations were sequenced on an Illumina HiSeq X Ten platform by Biomarker Technologies (Beijing, China) and paired-end reads were generated.