Single cell sequencing of placental tissue
Single-cell suspensions were prepared. The suspensions were then loaded onto microfluidic devices and scRNA-seq libraries were constructed according to the Singleron GEXSCOPETM protocol using the GEXSCOPETM Single-Cell RNA Library Kit (Singleron) (Dura et al., 2019). Individual libraries were diluted to 4nM and pooled for sequencing. Pools were sequenced on an Illumina HiSeq X with 150 bp paired end reads.