GSM5135254: 5dpi_PBS_5; Mus musculus; RNA-Seq
The medial prefrontal cortex was removed from frozen brain specimens. Total RNA was isolated using the SPLIT RNA Extraction Kit (Lexogen), according to the manufacturer's instructions. The TruSeq Stranded Total RNA Library prep kit (Illumina) with Ribosomal depletion, and dual barcoding (2x10 base pairs) was used to produce library constructs with an input of 400 ng total RNA.