RNA-seq of human Virus-specific T cells:EBV- YVL _donor25_forward_seq
Amplicons with identifiers were purified, quantified and pooled into one library for paired-end sequencing of 150bp on an Illumina HiSeq4000. Deep sequencing was performed at GenomeScan (Leiden, The Netherlands). Raw data were de-multiplexed and aligned to the matching TRBV, TRBD, TRBJ and constant (TRBC) genes. CDR3?-sequences were built using MIXCR software using a bi-directional approach (5-3 and 3-5 read