GSM5989872: HepG2_RNA_KO_ADRminus_rep1; Homo sapiens; RNA-Seq
For RNA-seq profiling, the total RNA was purified from HepG2_KO-TP53 cells with two independent samples, respectively. The total RNA was isolated from cells using the RNA isolation reagent TRIzol (Invitrogen). RNA libraries were prepared for sequencing using standard Illumina protocols Paired-end and strand-specific RNA-seq libraries were prepared according to Illumina's instructions and sequenced on HiSeq X Ten (ANOROAD) with a sequence length of 150 bp.