GSM5134626: HEK293-dCas13d-PPARG-g1-rep1; Homo sapiens; RNA-Seq
Total RNA was extracted with TRIZOL-based method, fragmented and reverse transcribed to cDNAs with HiScript Q RT SuperMix for qPCR (Vazyme, Biotech) according to manufacturer's instructions. RNA libraries were prepared for sequencing using standard Illumina protocols