Manatee_Temp2_Replicate1_Metagenome
Virus particles were concentrated from spring water via TFF & PEG. Virus particles were then purified from cellular debris and free nucleic acids through filtration and nuclease treatment. Viral DNA was extracted with the MinElute Virus Spin kit (Qiagen) and sheared using a Covaris instrument. NGS libraries were then prepared using the Accel-NGS 1SPlusDNA LibraryKit for Illumina Platforms (Swift Biosciences) and pair-end sequenced on a NextSeq platform.