GSM5136117: DC04 at T3 1; Homo sapiens; RNA-Seq
Replicate populations of viable tetramer+ CD8+ T cells were sorted into RLT Plus lysis buffer supplemented with 1% betamercaptoethanol and frozen at -80°C. RNA was extracted using QIAGEN AllPrep kits. Smartseq2 bulk protocol using oligo-dT primers to amplify cDNA from mRNA, followed by Illumina Nextera XT tagmentation and NextSeq 550 sequencing.