Whole Genome Sequence of PS900-tolerant L. monocytogenes mutant (HT014)
Listeria monocytogenes EGD-e WT, HT014 and HT015 strains were cultivated overnight in BHI-medium at 37C. Genomic DNA samples were prepared with overnight cultures (2 ODunit) using DNeasy Blood&Tissue Kit (QIAGEN) according to manufactures protocol. The genomic DNA samples were shipped to MicrobesNG (Birmingham, United Kingdom) for Illumina sequencing. Genomic DNA libraries were prepared using the Nextera XT Library Prep Kit (Illumina, San Diego, USA) and the libraries were sequenced using Illumina sequencers (HiSeq/NoveSeq) using 250 bp paired end protocol. Reads were adapter trimmed using Trimmomatic 0.30.