GSM4794645: YFP+_W4_scRNA-seq; Mus musculus; OTHER
Livers of control and DDC-treated mice were perfused with first 1X HBSS, followed by HBSS+ 1 mM EGTA, and HBSS + 5 mM CaCl2 with liberase. Following perfusion, livers were mechanically dissociated and incubated for 15 minutes in HBSS with CaCl2 and liberase at 37 degrees. Digests were filtered and cells isolated by FACS. After removing CD31, CD45, and CD11b cells, living cells were then sorted from the remaining cells (Bulk samples) or further sorted for YFP+ cells (YFP samples) Following isolation of sorted cells, single cell libraries were generated by the Center for Applied Genomics at the Children's Hospital of Philadelphia on a 10x Genomics chromium platform