GSM5530747: Experimental Media - D42B; Homo sapiens; ncRNA-Seq
Exosomal RNA was isolated from samples 4,5, 12,13 using the RNA Isolation Maxi Kit (Cat#60900) Total RNA was isolated for RNA seq using a RNeasy Mini Kit (Cat# 74104, Qiagen) according to the supplier's protocol. Barcoded total transcriptome RNA libraries were prepared by Ion Total RNA-Seq kit v2 (cat#4475936, Ion Torrent, ThermoFisher Scientific) according to manufacturer's protocols.