Metagenomic sequencing of CSF-5_SWGA
DNA was extracted from 200 l CSF specimens using QIAamp DNA Mini Kit (Qiagen). DNA was quantified by Qubit; 1 g SWGA-enriched DNA  was used for library preparation. The DNA solution was brought to 55 l with Tris buffer and fragmented by Covaris ultrasonicator (Covaris, US). The libraries were prepared by NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) using selection for 400-500 bp fragments and PCR amplification for 7-11 cycles. Multiplex sequencing of DNA libraries (4-12 libraries per run) was performed on an Illumina MiSeq using the 500-cycle V2 kit (Illumina, US) in accordance with the manufacturers protocol.