E.curvula_smallRNA:DonWalter
Starting from 30 mg of fresh tissue from each sample, ground to a fine powder using liquid nitrogen, the total RNA was extracted as two fractions, small and large RNA, which include RNA sequences smaller and larger than 200 bp, respectively, using a commercial NucleoSpin miRNA kit (Macherey-Nagel, Dren, Germany) according to the manufacturers instructions . The small RNA fraction was sequenced in biological triplicates, leading to three libraries: DW1, DW2 and DW3