GSM5989809: AFD; Homo sapiens; RNA-Seq
Human lumbar IVDs were retrieved from spines obtained with familial consent (IRB#s: A04-M53-08B and Tissue Biobank 2019-4896) through the Transplant Quebec Organ Donation Program. Discs dissected from the spinal column were graded according to Thompson grading system and used for tissue and cell isolation. Nucleus pulposus (NP) and inner annulus fibrosis (iAF) cells were isolated separately from degenerating and non-degenerating discs excluding outer AF. 500,000 NP or iAF cells from degenerating or non-degenerating discs were collected by centrifugation, and immediately applied to sc-RNA analysis 10X Chromium Single Cell 3'