GSM5208997: Panc1/imPSCc2 LS  rep4; Homo sapiens; Mus musculus; RNA-Seq
Total RNA was extracted directly from monospheroids and heterospheroids for RNA-sequencing using the RNeasy PLUS kit (Qiagen). Library preparations were conducted using NEBNext® Ultra™ II Directional RNA Library Prep Kit (NEB, E7760S), including a poly-A enrichment step by the NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB, E7490S). The End Repaired DNA was subjected to Agencourt AMPure XP bead clean-up and size selection (NEB, E6260). Libraries from the samples were multiplexed using NEBNext® Multiplex Oligos for Illumina® (NEB, Index Primers Set 1; E7335S).