Metagenomes from the Bedford Basin and the Scotian shelf: BBL3 Line
Water samples from different stations and depths of the Scotian Shelf were obtained during the 2016 fall and spring AZMP (Atlantic Zone Monitoring Program) cruises. ~4 L of water were collected at each station in Niskin bottles using a CTD Rosette and filtered through two consecutive polycarbonate filters (3.0 m and 0.2 m) to isolate microbial cells for DNA extraction. Samples were stored at -80C until their analysis. 42 representative DNA samples (0.2 m size fraction) from 9 stations were selected for metagenomic sequencing and analysis (Figure 2A; Table S2). DNA extraction was performed as described elsewhere (Zorz et al., 2019) and the samples were then sent to the Integrated Microbiome Resource (IMR) (https://imr.bio) for metagenomic sequencing via NextSeq (4X depth, 150+150 bp PE).