GSM6181400: SC-8cP1; Homo sapiens; RNA-Seq
Total RNA was extracted and purified by Zymo Research Quick-DNA/RNA MiniPrep Plus Kit (Zymogen) as per manufacturer's recommendations. Strand specific mRNA libraries were generated using the NEBNext Ultra II Directional RNA library prep Kit for Illumina (New England BioLabs #E7760), and mRNA was isolated using Poly(A) mRNA magnetic isolation module (New England BioLabs #E7490). Preparation of libraries followed the manufacturer's protocol (2.2 05/19).  Input was 1 µg and samples were fragmented for 15 min for RNA insert size of ~200 bp.  The following PCR cycling conditions were used: 98°C for 30 s / 8 cycles: 98°C for 10 s and 65°C for 75 s / and final extension of 65°C for 5 min.