GSM5484418: RNA-seq in EGR2 knock-out mouse alveolar macrophages treated in vivo with 300 μg zymosan (6hr, rep4); Mus musculus; RNA-Seq
The mice were euthanised by isoflurane inhalation then the cellular component were isolated through bronchoalveolar lavage. We sorted the CD45+ and F4/80+ alveolar macrophages and isolated total RNA using TriReagent. Libraries were prepared with the TruSeq ChIP library systems (Illumina) according to the manufacturer protocol.