GSM5901378: her3_38aa_72hpf_3; Danio rerio; RNA-Seq
All embryos were dechorionated at 24hpf. Approximately 25 embryos per sample were collected at 24hpf (samples 1-12) or 72hpf (samples 13-18). RNA was extracted with the QIAGEN Rneasy mini kit (Cat #: 74104) with on-column DNase digestion. Total RNA was spiked with ERCC spike-in (Cat #: 4456740). PolyA RNA was enriched with NEBNext® Poly(A) mRNA Magnetic Isolation Module (Cat #: E7490). NEBNext® Ultra™ II Directional RNA Library Prep Kit (Cat #: E7760) was used to prepare the library.