GSM5239687: IRF7 deficient macrophages stimulated with RANKL  rep1; Mus musculus; RNA-Seq
RNA from the cultures was extracted using a RNeasy Plus Mini Kit (Qiagen) according to the manufacturers instructions. Libraries were prepared following the manufacturer's procotols for the Illumina TruSeq Stranded mRNA library preparation kit (part # 20020595). Purified mRNA was isolated from total RNA using oligo-dT beads then converted into double stranded cDNA. Fragmented cDNA libraries were individually indexed and properly ligated molecules were PCR enriched to generate final library.