GSM5595984: 170814_AGP_XX_E11; Mus musculus; RNA-Seq
he presence of the Nr5a1-GFP transgene was assessed under UV light and genetic sexing was performed by PCR, as described previously . Urogenital ridges (E10.5, E11.5) from both sexes, and testes or ovaries, were isolated at each time point and enzymatically dissociated as described previously . Approximately 5000 single cells were loaded on a 10x Chromium instrument cRNA-seq libraries were prepared using the 10X Genomics Chromium Controller and Chromium Single Cell 3′ Library & Gel Bead Kit V2, as per the manufacturer's instructions. The prepared libraries were sequenced on an Illumina HiSeq4000 using paired-end 26 + 98 + 8 bp as the sequencing mode. Libraries were sequenced at a targeted depth of 100 000 to 150 000 total reads per cell. Sequencing was performed at the Health 2030 Genome Center, Geneva.