GSM6190479: DAC rep1 RNA; Homo sapiens; RNA-Seq
Total RNA was collected from cells using Trizol (ThermoFisher Scientific: 15596018) followed by an ethanol precipitation according to the manufacturerÕs protocol (Invitrogen). Extracted RNA was further purified by treatment with DNase (ThermoFisher Scientific: TURBO DNase, AM2238) to remove any trace DNA contaminants. Purity of RNA was analyzed by NanoDrop RNA-seq libraries were prepared (Roche: KAPA RNA HyperPrep Kit with RiboErase) according to manufacturer's protocol