GSM4844464: Set 1, control hiPSCs, replicate 2; Homo sapiens; RNA-Seq
Total RNA was purified using the Direct-zol RNA Miniprep kit (Zymo Research). 1 µg RNA was used for Poly(A) isolation using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, E7490L) and polyA mRNA was preppared for sequencing using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, E7420L).