GSM5018575: U2OS cells - HighMYC_rep1_HiC; Homo sapiens; Mus musculus; Hi-C
Human U2OS cells and mouse 3T3 cells were fixed with 2% formaldehyde using the Arima Hi-C Kit (Arima). 1 million fixed 3T3 mouse cells were added to each sample of 4 million human U2OS cells before subsequent steps of restriction enzyme digest, biotin end filling and ligation according to the Arima Hi-C protocol. Libraries were prepared using the KAPA Hyper-Prep Kit (KAPA), according to manufacturers' protocols.