GSM5596764: shCTRL_rep2_C4-2.smallRNA; Homo sapiens; miRNA-Seq
Total RNA was isolated from cells using the RNA MiniPrep kit (Direct-zol, R2052, ZYMO Research) with the procedure provided by the manufacturer.  RNA libraries were prepared for sequencing using standard Illumina protocols For RNA-seq, total RNA was isolated from cells using RNeasy Plus Mini Kit (Qiagen) and subjected to BGI for library preparation and sequencing using the DNBseq platform.For small RNA-seq, small RNA was extracted from cells using miRNeasy Mini Kit (Qiagen) and subjected to BGI for library preparation and sequencing using the DNBseq platform.