GSM5060404: LineOmock#16; Gallus gallus; RNA-Seq
total RNA was extracted using an RNeasy kit (Qiagen) according to the manufacturer's instructions. Each sample is from one individual bird. RNA libraries were prepared and sequenced using the Illumina HiSeq2000 system at the Beijing Genomics Institute (BGI, Hong Kong) using standard Illumina protocols.Poly (A) mRNA was purified from total RNA using magnetic beads with oligo (dT) (New England BioLabs, UK). The mRNA was then broken into short fragments using divalent cations at an elevated temperature. Using these cleaved RNA fragments as templates, random hexamer primers (Ivitrogen, Carlsbad, CA) was used for first-strand cDNA synthesis, followed by second-strand cDNA synthesis using DNA polymerase I (New England BioLabs, UK) and RNase H (Invitrogen).