GSM5327620: WT skin cells (CD45+ and CD45- cells) from DMBA-TPA treated mice; Mus musculus; RNA-Seq
Skin tissue (2 cm × 2 cm) was cut into small pieces of 1-2 mm and transferred to gentle MACS C-tubes (Miltenyi Biotec, Bergisch Gladbach, Germany) in 2.5 mL of complete RPMI medium with 2.5 mg collagenase type IV (Sigma-Aldrich) and 1.25 μl DNase I (Deoxyribonuclease Ⅰ, DP, Worthington Biochemical Corp, Lakewood, New Jersey, USA) at 37 ℃ for 1 h. Samples were filtered through a 70 μm cell strainer and spun down at 2,500 rpm for 5 minutes. 10X Genomics, Chromium Single Cell 3' Library and Gel Bead Kit v3.1.