Illumina NovaSeq 6000 paired end sequencing; RNA-seq of proximal, middle, and distal caudal fin tissue of thyroid-ablated zebrafish (hypothyroid, HypoTH) and wild-type zebrafish (euthyroid, EuTH)
Intact caudal fin tissue was sampled from sibling adults (>18 SL) reared under euthyroid or hypothyroid conditions. Each fish was first anesthetized with tricaine (MS-222, ~0.02% w/v in system water) and the entire caudal fin was amputated using a razor blade. Proximal, middle and distal regions of the fin were sampled immediately after and flash frozen in a dry ice-ethanol bath. Three biological replicates each containing five fin regions were collected for both TH backgrounds.  RNA was extracted the same day with Zymo Quick-RNA Microprep kit R1050 (Zymo Research, Irvine CA, USA). Sample libraries were made with NEBNext Ultra II RNA Library Prep kit