GSM5326683: T882; Mus musculus; RNA-Seq
RNA was extracted from tumor tissue using RNeasy mini kit (QIAGEN, Germantown, MD).  The integrity of RNA was assessed using Agilent Bioanalyzer and samples with RIN >8.0 were used. Libraries were constructed using NEBNext® Ultra™ RNA Library Prep Kits (non-directional) for Illumina, following manufacturer protocols. mRNA was enriched using oligo-dT beads. Libraries were sequenced on NovaSeq 6000 using paired-end 150 bp reads. There was no PhiX spike-in.