GSM4909471: ESC_RNA_rep2; Homo sapiens; RNA-Seq
Total RNA was extracted from 1 x 10^6 cells using the RNeasy Plus Mini Kit (Qiagen) as per manufacturer's instructions. RNA quality (RIN >9) was checked via Bioanalyzer (Agilent) and RNA concentration determined using a Qubit fluorometer (ThermoFisher). Libraries were prepared from 500ng RNA using the Truseq Stranded mRNA kit (Illumina), as per manufacturer's instructions and sequenced on a HiSeq 4000 (Illumina) platform (50bp, single-end).