GSM5903095: control 3; Drosophila melanogaster; RNA-Seq
Total RNA was extracted from larval cuticle preparations using a Qiagen RNAeasy mini prep kit as per the manufacturer's instructions. The total RNA was shipped on dry ice to the Columbia Genome Center (CGC; Columbia University, New York, NY) for standard sample processing and raw data analysis. RNAseq on mRNA-selected samples were performed by the Sulzberger Columbia Genome Center, Columbia University, using an Illumina HISEQ 2500 instrument. We obtained approximately 30 million single-end reads per biological replicate. The quality of the sequencing reads was checked using the FASTQC package and reads were mapped onto the Drosophila genome (release 6) using the STAR aligner package.