GSM5060341: H3K4me3_CX-5461; Mus musculus; ChIP-Seq
2x10^6 cells were cross-linked for 10 min with 1% final concentration fresh formaldehyde and quenched by 0.2M glycine for 5 min. Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to VAHTS Universal DNA Library Prep Kit for Illumina V3 Kit (vazyme, ND607-01). Briefly, input DNA was phosphorylated at 5' ends and dATP to yield a protruding 3-'A' base. After adapter ligation DNA was PCR amplified with Illumina primers for 15 cycles and library fragments of ~250 bp were purified by using DNA Clean Beads. Libraries were sequenced on the Illumina HiSeq 2500 following the manufacturer's protocols.