GSM5061579: Wild type GFP-MAB21L1 replicate 1 with treatment of tetracycline for 12 hrs; Homo sapiens; RNA-Seq
Tetracycline for 12 hours Total RNA extracted from two biological replicates of each cell line after 12 hrs of 1 μg/ml tetracycline treatment using the RNeasy kit (QIAGEN). Random primed cDNA from poly(A) selected RNA was converted into an Illumina sequencing library using RNA Library Prep Kit from Illumina (E7420, NEB, USA) in conjunction with NEBNext® Multiplex Oligos for Illumina (E7335/E7500, NEB, USA). Single-end 50-base pair (bp) reads were generated using a NextSeq 500 (Illumina Inc, SY-415-1002)