GSM5493489: RNA-Seq H9 (naïve) rep2; Homo sapiens; RNA-Seq
Flash frozen hESCs were thawed and spiked in with 5e4 Drosophila cells per sample and then total RNA was purified using the RNeasy Plus Mini Kit ( Qiagen; 74136) according to the manufacturer's protocol. Purified RNA quantities were estimated using the Qubit RNA HS assay kit (Life technologies; Q32852) and the samples were subsequently flash frozen. RNA-seq libraries were generated and sequence through BGI service for strand specific RNA-seq with PolyA selection (DNBseq Eukaryotic Transcriptome De novo Sequencing).