GSM5017894: ATAC-seq forelimb bud E10.5 replicate 2; Mus musculus; ATAC-seq
Per biological replicate, a pair of forelimb bud of mouse at E9.75 and wing bud of chicken at HH20, HH22 and HH24 stages were microdissected in cold PBS. Cells were isolated from the tissue using a Dounce Tissue Grinder (VWR KT885300-002) pestl A for 20 strokes followed by B 20 strokes in 1ml cold PBS. Cells were counted using a Neubauer Counting chamber (VWR 631-0696) 75'000 cells were processed per sample for ATAC-Seq as described in Buenrostro et al., 2015.