GSM4847761: EO771_Treg_ablated_rep1; Mus musculus; RNA-Seq
TAMs (defined as CD45+ CD11B+ Ly6G- F4/80+ cells) were sorted from EO771 orthotopic breast tumors as described, and collected into TrizolLS (Thermo Fisher) for subsequent RNA isolation. RNA isolation was performed following manufacturer's protocols. Initial quality control was performed using an Agilent Bioanalizer System through the Pathology Tissue and Data Acquisition and Analysis Core (TDAAC). All samples were sent to the Brigham Young University DNA sequencing core for further library construction and sequencing. KAPA Stranded mRNA-Seq Kit was used for library preparation. Samples were sequenced on the Illumina Hi-Seq 2500 according to Illumina's sequencing-by-synthesis protocol. 125bp paired-end reads were generated, yielding on average 87M reads per sample.