GSM6042859: 2747; Mus musculus; RNA-Seq
Total RNA was isolated using the Direct-zol RNA Microprep Kit (Zymo Research) followed by a DNase digestion step. Library preparation was carried out upon mRNA enrichment with the NEBNext Poly(A) mRNA Magnetic Isolation Module, using the NEBNext Ultra II RNA Directional Library Prep Kit for Illumina (New England BioLabs). Single read sequencing took place on a NextSeq 500 System (Illumina), using the corresponding NextSeq 500 High Output Kit v2.5, with a read length of 75 base pairs. The integrity of the RNA and quality of the library were assessed using a TapeStation 4200 (Agilent).