GSM6257103: M8 [scATAC-seq]; Homo sapiens; ATAC-seq
Nuclei were isolated from flash-frozen tumors following the 10x Genomics demonstrated protocol for Mouse Brain Tissue, with the following modifications: the first incubation in the lysis buffer was decreased to 45 seconds, and the second incubation, after pipet mixing, was 90 seconds. A 40 µm nylon cell strainer and a 30 µm MACS SmartStrainer (Miltenyi Biotec, cat. #130-098-458) were used in place of the suggested 70 µm and 40 µm tip strainers. Single-nulcei ATAC-seq cDNA libraries were prepared using the Chromium Single Cell Platform (10x Genomics) by the Center for Applied Genomics of the Children's Hospital of Philadelphia and sequenced on an Illumina NovaSeq 6000 sequencer using two 100-cycle SP flow cells.