GSM5811622: spinal cord, 1 week post transection 2 (ATAC-seq); Danio rerio; ATAC-seq
Sham injured and injured (2 mm rostral and caudal to the lesion site) spinal cords were dissected into a single cell suspension using 0.25% trypsin-EDTA; dissociated cells were processed for FACS sorting using an Astrios sorter, to collect live and single cells. 50.000 cells were processed for ATAC-seq. Library preparation as described in Buenrostro et al., 2015 Libraries were sequenced on the Illumina HiSeq 4000 platform, with over 40 million 150 bp paired-end reads obtained for each library.