GSM4974491: SEC1; Homo sapiens; ATAC-seq
To prepare nuclei, we spun 50,000 cells at 500g for 5 min, which was followed by a wash using 50 μL of cold 1× PBS and centrifugation at 500g for 5 min. Cells were lysed using cold lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2 and 0.1% IGEPAL CA-630). Immediately after lysis, nuclei were spun at 500g for 10 min using a refrigerated centrifuge. We followed the steps decribed in "Nature Methods volume 10, pages1213–1218(2013)". For both fibroblasts and ECs (from 6 patients and 6 controls), 50,000 cells were used for the Tn5 transposition reaction, and DNA libraries were prepared.