GSM5596719: Col0 rep2 (DNA seq); Arabidopsis thaliana; OTHER
Genomic DNA was extracted from sorted 16C nuclei using the ArcturusTM PicoPureTM DNA Extraction Kit (ThermoFisher Scientific, Waltham, MA). Samples were incubated at 65C overnight and at 95C for 10 minutes. The DNA samples were then purified using a genomic DNA Clean and Concentrator kit (Zymo Research, Irvine, CA). Sequencing libraries were generated at the Yale Center for Genome Analysis (YCGA) using the xGen Prism DNA library prep kit for NGS (Integrated DNA Technologies, Coralville, IA).