GSM5965740: H3K27ac, ChIP, Normal Stomach; Mus musculus; ChIP-Seq
Gastric corpus is dissected and epithelial cells are isolated using EDTA. Isolated epithelial cells were fixed and frozen by liquid nitrogen. Frozen tissues were thawed, lysed and sonicated to obtain genomic DNA, on which ChIP is performed. Immunoprecipitated DNA are eluted and purified using phenol/chloroform. Library is prepared using ThruPLEX DNA-Seq kit (Rubicon Genomics, R400428) according to manufacturer's protocol ChIP-Seq (50bp PE)