GSM5903323: Primary mouse astrocytes - p53 ChIP; Mus musculus; ChIP-Seq
ChIP was performed using the EZ-Magna ChIP kit (Millipore). Lysates were sonicated with Bioruptor Pico with 10-20 cycles (30s on, 30s off at 4°C) until chromatin was average of 100-400bp. IP was performed overnight at 4°C with p53-CM5 antibody (Leica, 1:50) or 1mg Normal Rabbit IgG. Libraries were prepared with NEBNext Ultra II DNA Library Prep kit per maufacturers instructions, with 13 cycles of PCR amplification. Libraries were pooled and sequenced on Illumina NextSeq 500 platform with NextSeq 500/550 Mid Output Kit v2.5 (150 cycles) with Paired End 2x75.