GSM5492241: B412_Replicat1_pos_Zn_pos_GSK_RNAseq; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNeasy Plus Mini kit (Qiagen) U937 cDNA was synthesized using a SMART-Seq v3 Ultra Low Input RNA kit for Sequencing (Clontech). ds-cDNA was fragmented using S220 Focused ultrasonicator (Covaris) and libraries were generated using a NEBNext Ultra DNA Library Prep kit (New England Biolabs).