GSM5866909: wt_d3_ATAC; Mus musculus; ATAC-seq
50.000 cells were centrifuged (500 x g, 5 min, 4ºC), washed in ice-cold PBS and lysed in cold lysis buffer. Nuclei were then pelleted and used as input material for tagmentation. Tagmentation with the Nextera DNA Library Prep Kit (Illumina, FC-121-1030), followed by DNA purification with the MiniElute PCR Purification Kit (Qiagen, 28004). Amplification and barcoding with the NEBNext High Fidelity 2X PCR master Mix (New England Labs, M0541).