GSM6042686: TE5_NUP153; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching, chromatin received enzymatic digestion by following manufacturer's protocols  (CST, SimpleChIP plus enzymatic chromatin IP kit). ChIP-seq libraries were prepared using the KAPA Hyper Prep Kit. 10 ng of DNA was used as starting material for ip samples. Libraries were amplified using 12 cycles on the thermocycler. Post amplification libraries were size selected at 250-450bp in length using Agencourt AMPure XP beads from Beckman Coulter. Libraries were validated using the Agilent High Sensitivity DNA Kit.