GSM5768311: metl-9 KO normal condition (OP50)_2; Caenorhabditis elegans; RNA-Seq
Total RNA were extracted by Trizol reagent and chloroform extraction. 1)mRNA enrichment and purification: Oligo dT Selection to enrich the mRNA (For total RNA extracted from human whole blood, globin mRNA are depleted ); 2)RNA fragmentation and cDNA synthesis (second-strand cDNA synthesis with dUTP instead of dTTP); 3)End repair, add A and adaptor ligation; 4)PCR; 5)Circularization and make DNB; 6) Sequencing on DNBSEQ platform