NextSeq 500 paired end sequencing; RNA-seq of human multiple myeloma cell lines JJN3, OPM2 and XG7 with PRMT5 inhibitor EPZ015938 versus untreated controls.
Human multiple myeloma cell lines were cultured for 3 days with either DMSO or 5 ÂµM of EPZ015938. Cells were harvested at the end of experiment and kept on ice before RNA extraction. RNA was extracted by using silica based spin columns. RNA quality and integrity was checked through the RIN-value for each sample. RNA-seq library construction was performed with 150 ng of input RNA, using the Illumina TrueSeq Stranded mRNA Library Prep Kit.