GSM4974484: KO_MPP_H2B-GFP_LOW_RNA_rep_3; Mus musculus; RNA-Seq
HSCs and MPPs were sorted into Trizol and RNA purified according to the manufacturer's instructions. DNase I-treated RNA samples were purified using the QIAGEN MinElute kit before cDNA was made and amplified with a SMART-Seq2 kit. The cDNA was then fragmented and barcoded for sequencing using a Nextera XT kit.