GSM5059963: D2RNAU; Homo sapiens; RNA-Seq
Monocytes were lysed immediately (0hr) using QIAzol lysis reagent (Qiagen) or after culturing for 2, 10 or 24 hours. Total RNA was isolated using miRNeasy mini kit (Qiagen) as per manufacturer's protocol. Total RNA samples were enriched for mature, poly-A mRNA transcripts and prepared for sequencing using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina. Two runs of 150 base pair paired-end sequencing were carried out per sample on the NovaSeq 6000 (Illumina) platform with v1.0 chemistry.