GSM5491987: polyA Enriched RNA-sequencing_p28_Rnaseh2b_Atm_CB_5; Mus musculus; RNA-Seq
Total RNA was isolated from tissue with TRIzol (Invitrogen). Library preparation using TruSeq Stranded Total RNA Kit (Illumina). Double stranded cDNA fragments were ligated with Illumina paired-end adaptors, followed by size selection (~200bp) and libraries were analyzed using a HiSeq 2000 sequencing system (Illumina).