GSM5492288: ATAC-seq NKG2A negative NK cells; Mus musculus; ATAC-seq
The ATAC-seq Tn5 transposase reaction was performed on a crude Igepal CA-630 nuclear prep from ~50,000 NK cells. CUT&RUN experiments were performed on ConA-bead immobilized cells permeabilized with a 0.05% Digitonin solution. ATAC-seq libraries were constructed using Nextera compatible primers. CUT&RUN libraries were constructed using the New England Biolabs Ultra II DNA library prep kit for Illumina