GSM4909931: Day 14D rep1; Homo sapiens; RNA-Seq
Aggregates were dissociated with accutase at 37ºC for 7 min. After enzymatic neutralization, the cell pellet was washed with phosphate buffered saline (PBS, 0.1M) and then stored at -80ºC. Total RNA was extracted from samples using High Pure RNA Isolation Kit (Roche, Cat. 11828665001), according to the manufacturer instructions. RNA libraries were prepared for deep sequencing, starting with 500 ng of Total RNA and following Lexogen QuantSeq 3'mRNA-Seq Library Prep Kit FWD for Illumina using standard protocols. 3'Tag RNA-seq