GSM6042810: ChIPseq_H3K9me3_NPC_464-2WT; Mus musculus; ChIP-Seq
100,000 nuclei from GE_NPC were used for ChIPseq library preparation. Native ChIP-seq were perform according to previous publication (Kundakovic et al., 2017). After incubated with A/G Magnetic beads (Thermo Scientific™, #88803), ChIP DNA was went through end repairing (End-it DNA Repair Kit, Epicentre, ER0720), A-tailing (Klenow Exo-minus, Epicentre, KL06041K), adapter ligation (Fast-Link kit (Epicentre, LK11025) using VAHTS DNA Adapters set2  (Vazyme, N802-01), and then PCR amplified using NEBNext 2X master mix (E7335S). SPRIselect beads (Beckman, B23318) was used for library size selection (1:0.55 followed by 1:1.12). The final library concentration was measured with Qubit4, and the quality was evaluated on Agilent 4200 TapeStation, and then sent for sequencing.