GSM5710158: aTCR rep2; Homo sapiens; RNA-Seq
Different T cells were cocultured with GPC3 peptide for four hours. Total RNA was extracted from T cells using TRIzolTM Reagent (Invitrogen) and the RNA quality was checked using an Agilent Bioanalyzer 4200 (Agilent technologies). Then, the total RNA was purified with the RNA Clean XP Kit (A63987, Beckman Coulter) and DNase set (79254, QIAGEN). Sequencing libraries were generated using VAHTS® mRNA-seq V2 Library Prep Kit for Illumina (NR603, Vazyme) according to the manufacturer's instructions.