GSM6042685: TE5 cells, shNUP153, rep2; Homo sapiens; RNA-Seq
RNA was harvested usingTotal RNAs were extracted with NucleoSpin RNA (MACHERY-NAGEL). Initial RNA quality were checked by electrophoresis and sent to NOVOGENE for sequencing. 1 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using 250-300 bp insert cDNA library following manufacturer's protocols.