GSM4972275: C3A_neg; Homo sapiens; RNA-Seq
Nuclei were isolated from fresh frozen human brain tissue using a sucrose gradient ultracentrifugation protocol. Nuclei were separated into 3 populations using FANS: 1) NEUNpos neurons used for bulk RNAseq, 2) OLIG2pos oligodendrocytes and OPCs used for bulk RNAseq, 3) NEUNnegOLIG2neg nuclei used for single-nucleus RNAseq. For bulk RNAseq RNA was isolated with the Arcturus™ PicoPure™ RNA Isolation Kit from Thermo Fisher Scientific Bulk: Lexogen QuantSeq 3' mRNA-Seq Library Prep Kit (FWD with UMI) from Illumina. snRNAseq: Chromium Single Cell 3' Reagents Kit v3 from 10X Genomics