GSM5060686: K107013 MeCap; Homo sapiens; Bisulfite-Seq
Total DNA was extracted using the Allprep DNA/RNA/miRNA Universal Kit (QIAGEN, Germantown, MD). The concentration and quality of total DNA samples was first assessed using Agilent 2100 Bioanalyzer. For the DNA methylation analysis: Library was generated using MethylCap Library Kit (Diagenode, Denville NJ, US) according to the manufacturer′s protocols followed by single-end 75-bp sequencing on Illumina Nextseq 500.