GSM5710556: CCR2-DTR Trichinella-Infected; Mus musculus; RNA-Seq
Brain total cellular RNA was extracted using TRIzol reagent according to the manufacturer's instructions (Thermo Fisher Scientific). The quality of RNA was first checked for integrity on an Agilent Bioanalyzer 2100; samples with RNA integrity number (RIN) >7.0 were used for subsequent processing. Total RNA was subjected to two rounds of poly(A) selection using oligo-d(T)25 magnetic beads (New England Biolabs). Illumina compatible RNAseq library was prepared using NEB next ultra RNAseq library preparation kit. The cDNA libraries were purified using AmpureXP beads and quantified on an Agilent Bioanalyzer and qubit analysis. RNA libraries were prepared for sequencing using standard Illumina protocols