GSM5869945: RB10; Homo sapiens; RNA-Seq
From cell pellets, total RNA was extracted and cDNA synthesized using the SMART-Seq® kit (Takara Bio USA, Inc.) according to the manufacturer's protocol Sequencing libraries were generated using Nextera XT DNA Library Preparation Kits (Illumina, Inc.). The manufacturer's protocol was modified to accommodate a reduced amount of starting material (100-150 pg). Adapter indices were used to multiplex the libraries, which were pooled prior to cluster generation using a cBot instrument. The loaded flow-cell was then paired-end sequenced (76 + 76 cycles, plus indices) on an Illumina HiSeq4000 instrument. Finally, the output data was demultiplexed (allowing one mismatch) and BCL-to-Fastq conversion performed using Illumina's bcl2fastq software.