GSM5328123: Oxidative mECs ATf4-/- rep1; Mus musculus; RNA-Seq
ECs were directly sorted (70 μm nozzle) into 700 μl RNA lysis buffer, and RNA extraction was performed using RNeasy Plus Micro Kit (74034 QIAGEN). The libraries were prepared following Illumina TruSeq stranded mRNA protocol. The quality of the RNA and final libraries was determined using an Agilent 4200 TapeStation System. The libraries were pooled equimolecularly and sequenced in an Illumina NovaSeq sequencer (single-end 100 bp) with a depth of around 20 Mio reads per sample.