GSM5767997: scRNA-seq-Ctrl1; Mus musculus; RNA-Seq
Freshly harvested control, acute infected and chronic infected mouse lungs were digested by Lung Dissociation Kit (MACs# 130-095-927) and generate single-cell suspension according to its protocol.Single cells were suspended in 1X PBS (calcium and magnesium free) containing 0.04% weight/volume BSA (400 µg/ml). A part of single-cell suspensions were used to conduct scRNA seq, other part of them were used to perform bulk-RNA seq. RNA libraries were prepared for sequencing using standard Illumina protocols