GSM4973230: PEO23 R 00Hr rep2; Homo sapiens; RNA-Seq
Total RNA was extracted from cell pellets with the miRNeasy Kit (Qiagen, cat#217504). Purified total RNA samples were evaluated for quality and quantity by Agilent Bioanalzyer using RNA 6000 Nano chip and reagents (cat#5067-1511). Sequencing libraries were prepared using Illumina TruSeq stranded Total RNA sample preparation kit (cat# 20020597) from 200ng of RNA according to manufacturer's protocol. The finished libraries were quantified by Qubit fluorometer, Agilent TapeStation 2200 D1000 screentape (cat#5067-5582), and RT-qPCR using the Roche Kapa Biosystems library quantification kit (cat#KK4854) according to manufacturer's protocols. Whole genome libraries for copy number analysis were sequenced with >25M 75bp read pairs and RNAseq libraries were sequenced with >50M 75bp read pairs by the Molecular Biology Core Facilities at Dana-Farber Cancer Institute.