GSM5866523: LNCaP WT cells- CHARGEseq_GCN2iB_EAA_8hr_2; Homo sapiens; ncRNA-Seq
For CHARGE seq, total RNA was extracted with TRIzol (Life Technologies) according to manufacturer's protocol followed by tRNA extraction as mentioned in the methods tRNA library was made in the lab as described in the methods and library was submitted to Genewiz (South Plainfield, NJ) where they performed sequencing