GSM5018544: DMSO_2; Mus musculus; RNA-Seq
total RNA was isolated from satellite cell cultures using the RNeasy® Micro Kit (Qiagen) The RiboGone™-mammalian (Takara Bio) kit was subsequently used to eliminate ribosomal and mitochondrial RNA. cDNA libraries for RNA-sequencing were then prepared using the SMARTer® Stranded RNA-Seq (Takara Bio) kit. The resulting cDNA libraries were sequenced with the Illumina MiSeq system (Genome Quebec).