GSM6255769: K562, KO, biological replicate 3; Homo sapiens; RNA-Seq
RNA was extracted using Agencourt RNAdvance Tissue kit including DnaseI (Ambion) treatment for 15 min at 37oC on an automated Biomek i7 Hybrid system  (Beckman Coulter).  RNA quantification and quality control was performed on Fragment Analyzer 5300 system and showed an RNA integrity number (RIN) of  10 for all the samples. 500ng of input RNA was used per sample mRNA-seq library preparation was performed using KAPA Stranded mRNA-Seq Kit, with KAPA mRNA Capture Beads (Roche) following manufacturer's protocol with 500 ng of input RNA, 5 min fragmentation time at 95oC, 10 PCR cycles, and multiplexed using TruSeq UD-UMI adapters. Resulting libraries were pooled equimolarly to a final concentration of 1.9 nM.