GSM5021201: 92-16: UMUC3 100 DEC 3 days_1; Homo sapiens; RNA-Seq
Rneasy Mini Kit, Qiagen according to manufacturer with optional DNAse treatment Libraries were prepared according to Vazyme instructions accompanying the VAHTS™ Universal V6 RNA-Seq Library Prep Kit for Illumina. Briefly, 300 ng total RNA were used for mRNA capturing, fragmentation, the synthesis of cDNA, adapter ligation and library amplification. Bead purified libraries were normalized and finally sequenced on the HiSeq 3000/4000 system (Illumina Inc. San Diego, CA, USA), following the manufacturer's protocols, with a read setup of 1 × 150 bp.