BGISEQ-500 paired end sequencing; RNA-seq of Sitobion avenae body parts and developmental stages
The maternal Grain Aphid was collected in 2017 from a wheat field in Carlow, Ireland, and removed from the colony and stored after producing nymphs. The aphid colony was reared on barley plants in an insect incubator at 20 °C and 16/8 h day-night circle. Genotyping of the original maternal aphid and aphids from following generations confirmed that the aphids were the partially resistant SA3 clonal lineage. RNA was extracted using the GenElute™ Mammalian Total RNA Miniprep Kit (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany). 50 aphids per sample were used to perform RNA extractions.  Oligo dT Selection to enrich the mRNA; stranded specific mRNA libraries，second-strand cDNA synthesis with dUTP instead of dTTP