GSM6043267: IT325_P1_11; Danio rerio; RNA-Seq
Three months old wild-type and mutant adult zebrafish were anaesthetized with 0.02% tricaine before kidney collection. A ventral, midline incision was made and kidney were dissected and placed into ice-cold 0.9× PBS containing 5% FCS. Single-cell suspensions were generated by aspiration followed by gentle 'teasing' of each organ on a 40-μm nylon mesh filter with a plunger from a 1-ml syringe. As described in CEL-Seq2 protocol (Hashimshony et al. 2016) Adapted from TruSeq Small RNA Library Preparation Protocol