GSM4909774: Lung - mRNA_CB_Day1_Rep2; Mus musculus; RNA-Seq
RNA was isolated from whole lung homogenate after pulverizing the lung tissues at -80 ° C. Total RNA was isolated using RNeasy Mini Kit (Qiagen 74106) and reverse transcription was performed using manufacturer's protocol. Libraries were built with the TruSeq® Stranded mRNA Library Prep Kit (Illumina, San Diego, CA) and run on the HiSeq 2500 (Illumina) in 51bp paired end reads. Fastq files were mapped and quantified using the mapping-based mode of Salmon 1.1.0.