GSM4909043: Gm4665_1_S1_L001; Mus musculus; RNA-Seq
we employed bilateral testicular microinjection: one testis was injected with AAV9-shCtrl-RFP; the contralateral testis was injected with AAV9-shRNA-RFP. We removed somatic cells and isolated RFP+ germ cells infected with AAV9-RFP from shCtrl or Gm4665-depleted testes by FACS, before performing scRNA-seq. Single-cell RNA-seq libraries were prepared using Single Cell 3' Library Gel Bead Kit V2 (10x Genomics) following the manufacture's introduction.