GSM5018567: F1_S1_L006_R1_001; Geobacter lovleyi; RNA-Seq
Total RNA extraction following manufacturer's recommendation for QIAGEN RNeasy kit (QIAGEN) rRNA depletion used an Illumina TruSeq Total RNA Library Preparation kit with RiboZero Bacteria rRNA depletion beads. Libraries were quantified using Qubit and Advanced Analytical Fragment Analyzer High Sensitivity DNA NGS assays. The libraries were then pooled in equimolar amounts for multiplexed sequencing and the pool was quantified using the Kapa Biosystems Illumina Library Quantification qPCR kit.