GSM4972351: S71ABIC Replicate3; Rattus norvegicus; RNA-Seq
RNA was extracted from neuronal cultures using the RNeasy Micro Kit (Qiagen) according to manufacturer's instructions. Libraries were prepared with Nugen Universal plus mRNA-Seq Kit (Nugen) to generate strand-specific RNA-seq libraries. Samples were multiplexed, and sequencing was performed on Illumina HiSeq 3000 to a depth of 25 million reads/sample with single-end 65  bp reads.