GSM4973502: Fxr-intKO - 75; Mus musculus; RNA-Seq
Tissue was lysed using TRIzol reagent, whereafter the RNA was further processed from the aqueous phase using the Qiagen RNEasy kit, including treatment with DNaseI. mRNA was enriched from total RNA using the Poly(A) Beads (NEXTflex). Sequencing libraries were prepared using the Rapid Directional RNA-Seq Kit (NEXTflex) acording to the providers protocol