GSM5020833: Pu.1-URE-/- 1; Mus musculus; RNA-Seq
Flk2- Lin-Sca-1+Kit+ cells were FACS-sorted from murine bone marrow aspirates and subject to total RNA extraction using RNeasy micro kit. Total RNA was subjected to mRNA enrichment, fragmentation, cDNA synthesis, adapter ligation, and PCR amplification for library construction, with incorporation of the DNA nanoball technology (DNB-Seq). RNA-seq was performed by BGI Americas using the BGISEQ-500 platform, paired-end 100-bp read length.