GSM5328291: FT rep3; Francisella tularensis subsp. tularensis SCHU S4; RNA-Seq
After the culture of Francisella strains in the growth media, cell pellets were harvested using NucleoSpin RNA (Takara Bio, Cat#740955). After removal of rRNA using Illumina Ribo-Zero Plus rRNA Depletion Kit(Cat#20037135), TruSeq Stranded mRNA Library Prep Kit(Cat#20020595) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols