GSM6189427: Card14KI, scRNAseq; Mus musculus; RNA-Seq
The full-thickness skins of the back from one WT and one Card14KI mice at one year after birth were excised surgically. The two samples digestion was performed using the whole skin dissociation kit for human (Miltenyi Biotec, Germany). Enzymatic digestion was completed in 73 min, followed by mechanical dissociation using gentleMacs Octo Dissociator with heaters (Miltenyi Biotec), running the gentle MACS mice program 37C_m_Bo_WSDK_2. Next, dead cells and debris were removed, using the dead cell removal kit (Miltenyi Biotec). Preparation of the scRNAseq library, sequencing and data preprocessing were outsourced to AZENTAⓇ corporation Japan branch office. Single cell cDNA libraries were prepared by using 3' Gene expression v 3.1(10X Genomics), and the constructed libraries were sequenced on DNBSEQ-G400 (MGI-Tech, China) with paired end 150 bp. Raw sequencing data were processed via Cell Ranger (10X Genomics)