GSM5492050: ATI_inj_4; Mus musculus; RNA-Seq
The Achilles tendon was separated from soleus muscle and were homogenized using a tissue homogenizer, and total RNA was extracted using TRI reagent and the manufacturer's protocol. The sequencing library is prepared by random fragmentation of the DNA or cDNA sample, followed  by 5' and 3' adapter ligation. Alternatively, “tagmentation” combines the fragmentation and ligation  reactions into a single step that greatly increases the efficiency of the library preparation process.  Adapter-ligated fragments are then PCR amplified and gel purified.