GSM5210424: SC (controlled group); Rattus norvegicus; RNA-Seq
The tissue was transferred to the complete medium (aMEM + 20% FBS + P/s) and then crushed into cell suspension.Using single BD Rhapsody™ Cartridge Reagent Kit (Cat. 633731), the cell suspension (300-600 living cells per microliter determined by Count Star) were then further pooled and passed over a sample cartridge (400000847)for the BD Rhapsody to trap single cells. Processing for RNA isolation and cDNA library preparation according to the manufacturer's protocol. Single-cell RNA-seq libraries were constructed according to the manufacture's introduction, The libraries were finally sequenced using an Illumina Novaseq6000 sequencer with a sequencing depth of at least 100,000 reads per cell with pair-end 150 bp (PE150) reading strategy (performed by CapitalBio Technology, Beijing).