GSM5493079: p_386; Homo sapiens; RNA-Seq
PBC RNA was extracted according to the manufacturer's protocol. RNA integrity was measured on an Agilent 2100 Bioanalyzer (Agilent Technologies).   mRNA was enriched from total RNA using oligo(dt) beads (NEBNext Ultra II RNA Kit following the poly(A) enrichment workflow). The mRNA was subsequently fragmented randomly in fragmentation buffer, and reverse transcribed to cDNA.  The cDNAs were converted to double stranded cDNAs, then subjected to end-repair, A-tailing, and adapter ligation, size selection and PCR enrichment. Libraries are pooled into Novaseq6000 machines according to molarity and expected data volume.