GSM5492245: scRNA_PLZF_RARA_Ctrl; Mus musculus; RNA-Seq
PLZF/RARA transformed progenitors (late promyelocytes: Cd45.2+, C-Kit+, Gr1+, Cd34-, FcγR+) were purified using the FACS Aria III cell sorter (Beckman Dickinson). FACS purified  promyelocytes were loaded 30 min after the sorting onto a chromium Single Cell Chip and processed with the Chromium Controller (10x Genomics) according to the manufacturer's instructions for single cell barcoding at a target capture rate of 6000 individual cells per sample.  Libraries were prepared using Chromium Single-Cell 3′ Reagent Kits v2 (10x Genomics).