GSM4972185: ExoSeq_KCl_Rep1; Mus musculus; RNA-Seq
Total RNA was extracted as described previously and depleted from rRNA and SRP-RNA The 5' ExoSeq library construction was performed as described (Afik et al., 2017), with the following alterations. Instead of poly(A)-selected RNA we started with a rRNA and signal recognition particle (SRP)-RNA depleted RNA sample. rRNA & SRP-RNA was depleted using a commercially available kit (RiboCop rRNA depletion kit V1.2 (human/mouse/rat), Lexogen) according to the manufacturer's instructions with some modifications. For one 5' ExoSeq library sample, RNA from three depletion reactions (3x 500 ng total RNA) was pooled before library construction.