GSM6189524: ATAC_T8_WT_H_H_RMMF209; Mus musculus; ATAC-seq
Chromatin accessibility mapping by ATAC-seq was performed as previously described (Buenrostro et al., 2013; Corces et al., 2016), with minor adaptations. After centrifugation, the pellet was carefully resuspended in the transposase reaction mix (12.5µl 2xTD buffer, 2µl TDE1 (Illumina), 10.25µl nuclease-free water, and 0.125µL 10% NP-40 (Sigma) for macrophages and dendritic cells or 0.25µl 1% digitonin (Promega) for all other cell types) for 30 min at 37 °C. Following DNA purification with the MinElute kit eluting in 11µ