GSM5492011: S-OE2-2; Nicotiana tabacum; RNA-Seq
At 4 DAT, the 5th leaf blades (with main veins removed) of four individual plants under control conditions and saline conditions in the daytime were sampled and frozen immediately in liquid nitrogen. Leaf blades from four individual plants were mixed and ground to powder. RNA was isolated and purified with RNeasy Plus Mini Kit (Qiagen, Cat No./ID: 74134, the Netherlands) following the manufacturer's protocol. RNA samples of WT and OE-2 overexpression lines from two independent salt treatment experiments were used for transcriptome sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols