GSM4910216: mmMO 3: Control Morpholino Replicate 3; Gallus gallus; RNA-Seq
Cranial neural fold explants were placed in Ringer's saline on ice for a maximum of 1 hour. Neural fold explants were homogenized in TRIzol Reagent (ThermoFisher Scientific, Waltham, MA) and stored at -80°C until bilateral cranial neural folds from 60 embryos of each condition were collected. These homogenized samples were then pooled in groups of 12, creating five biological replicates for each condition. Total RNA was isolated by TRIzol extraction, then cleaned by following the RNA Cleanup protocol with RNeasy spin columns (QIAGEN, Velencia, CA). Poly(A)+ RNA was selected from total RNA using the Illumina TruSeq stranded mRNA kit (San Diego, CA).