GSM5904523: sgScr-B; Homo sapiens; RNA-Seq
RNAeasy Mini Kit (Qiagen), according to the manufacturer's instructions. RNA quality was defined on an Agilent 2100 Bioanalyzer, then processed with Ribo-Zero rRNA Removal Kit (Illumina) to remove rRNA, and further processed into sequencing libraries using the Illumina ScriptSeq Complete Gold kit following manufacturer's protocol.