GSM5965383: E.coli_ALE_2; Escherichia coli str. K-12 substr. MG1655; RNA-Seq
Cell culture samples were harvested at the mid-log growth phase (OD600 ~0.5 for MG1655 and ALE-1 strain). The RNA was stabilized using by RNAprotect® Bacteria Reagent (Qiagen #76506) and total RNA was isolated using RNeasy® Plus Mini Kit (Qiagen #74134). Ribosomal RNA (rRNA) was removed from isolated RNA samples through riboPooL Probes (Pan-prokaryote) siTOOLS BIOTECH and Dynabeads™ MyOne™ Streptavidin C1(Invitrogen #65001). For purification of RNA samples, RNA clean & concentrator (zymoresearch R1013) was used. RNA library was constructed from rRNA-depleted RNA using the KAPA stranded RNA-seq kit (Roche KK8400).