GSM5769013: H10B1_6-gut-CL; Apis mellifera; RNA-Seq
Guts were thawed on ice and homogenized in a FastPrep-24 5G homogenizer (MP Biomedicals) at 6 m/s for 45 s in 1 ml 1X PBS containing ca. 100 µl of 0.1 mm Zirconia/Silica beads (Carl Roth). Half of the volume of these homogenates was used for RNA extraction. RNA was extracted with a hot phenol protocols as described in Kešnerová et al. (2017), doi:10.1371/journal.pbio.2003467 RNA-sequencing libraries were prepared with the KAPA stranded mRNA kit (Roche) following the manufacturer's protocol, except that we appended TruSeq unique dual indexes (UDIs, Illumina) instead of the adapters provided by the kit to better control for index hopping during sequencing.