GSM6043548: Hbt sal: NEBHvo_60 - rep1; Halobacterium salinarum; RNA-Seq
~3-5mL of cells in mid-log phase were flash-frozen in Liq N2 and stored at -80C. Total RNA was extracted from cell pellets using the Agilent Absolutely RNA Miniprep kit. RNA was measured using Nanodrop and quality-checked using Bioanalyzer. 1-10ng of total RNA was used as input for rRNA removal by the various kits tested here. The kit protocol included creation of a dsDNA library from the depleted RNA as template. This library was quality-checked using DNA High Sensitivity chip on the Bioanalyzer.