GSM5767968: non-HF-2E2; Homo sapiens; RNA-Seq
Total RNA was extracted from freshly frozen epicardial adipose tissue using a QIAGEN miRNAeasy Mini Kits (Qiagen, Hilden, Germany) according to the manufacturer's protocol. Isolated total RNA was subjected to ribosomal RNA deletion using a Ribo-Zero Magnetic Kit (EpiCentre, Madison, WI), randomly fragmented and then used to construct RNA sequencing library using KAPA Stranded RNA-Seq Library Prep Kit for Illumina. The RNA fragments were primed with random hexametric primers to synthesize the first cDNA strand, and the second cDNA strand was incorporated with dUTP instead of dTTP. The resulting cDNA were amplified in situ using TruSeq SR Cluster Kit v3-cBot-HS. The strand-specific libraries were qualified on the Agilent 2100 Bioanalyzer and subsequently sequenced on the Illumina HiSeq 6000 platform.