GSM5493327: CLA11-24h [single-cell RNA-seq]; Mus musculus musculus x M. m. castaneus; RNA-Seq
Cells were dissociated with accutase for 5 minutes, washed 3 times with DPBS and resuspended in PBS containing 0.04% BSA. Cell concentration was adjusted between 800 to 1200 cells/μl and cell suspension was kept on ice before loading on the 10x Genomics Chromium instrument. scRNA-seq libraries were generated using the Chromium single cell 3' reagent kit V3.1.