GSM5060369: ventral_SNpc_HC7; Homo sapiens; RNA-Seq
RNA was extracted from ~100 pooled LCM neurons from each tier using PicoPure RNA isolation Kit (Arcturus, Mountain View, CA) with DNAse digestion step (RNAse-Free DNAse Set; QIAGEN, Hilden, Germany). The cDNA was prepared for the sequencing using the SMARTer Kit (Clontech Laboratories, Mountain View, CA). Samples were sequenced (HiSeq4000 75bp paired-end) across 2 lanes