GSM6043039: premets_27; Homo sapiens; RNA-Seq
Total RNA was isolated from   PBMC cell using TRIzol® RNA Isolation Reagents (Life technologies, Carlsbad, CA) based method. The quantity and quality of the RNA were evaluated using Agilent Nono Kit and assessing the RNA integrity number (RIN >7). One mg of total RNA was processed for preparing mRNA sequencing library using MGIEasy RNA Directional Library Prep Kit (MGI) according to manufacturer's instruction. RNA libraries were prepared for sequencing using standard MGI protocols