GSM5326924: Ribo_vec_Tg50; Homo sapiens; OTHER
Cells were directly lysed in lysis buffer on culture dishes. For ribosome profiling, RNase I treatment, ribosome pelleting, size selection, preadenylated linker ligation, rRNA depeletion, reverse transcription, cDNA circularization, and PCR amplification. For RNA-Seq, TruSeq Stranded mRNA Library Prep Kit (Illumina). Ribosome profiling and RNA-seq