GSM5021241: Patient 005_HS196 positive cells; Homo sapiens; RNA-Seq
Tumors were processed into single cell suspensions as described in manuscript, and top 2% HS196+ and bottom 25% HS196- cells were sorted. 10X Genomics gene expression and TCR libraries were generated using Chromium Single Cell 5' Library Construction Kit (V1.1). A targeted cell recovery of 7,000 cells were used for each sample. scRNA seq (10X Genomics), Single Cell 5' Gene Expression Libraries, 26x8x98 single i7 index. GEX sequenced on NovaSeq 6000 (500M PE-reads/sample)