GSM5059933: G_0_hrs; Homo sapiens; ATAC-seq
50,000 monocytes per sample were harvested immediately (0hr) or 2, 10, 24 hours post-culture. Cells were centrifuged at 300 xg for 5 minutes at 4°C and each sample resuspended in a transposase reaction mix (12.5 μL 2x TD buffer, 2 μL TDE1 (Illumina)), 10.25 μL nuclease-free water and 0.25 μl 1% digitonin (Sigma-Aldrich) and incubated for 30 minutes at 37°C. 1 μL of eluted DNA from each sample was used in a quantitative PCR (qPCR) reaction to estimate the optimum number of amplification cycles for library amplification. Each sample library was amplified for the number of cycles corresponding to the test qPCR Cq value, using custom Nextera primers (Buenrostro et al., Nat Methods 10, 1213–1218, 2013). Library amplification was followed by Solid Phase Reversible Immobilization (SPRI, Beckman Coulter) size selection to exclude fragments >1,200 bp. Paired-end, 75bp sequencing was carried out on an Illumina HiSeq 3000/4000 instrument  by the Biomedical Sequencing Facility at CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences.