GSM4909220: PFF_rep1; Sus scrofa; RNA-Seq
The total RNA of 6-well PFFs is extracted by TRIzol. The total RNA of 30 SCNT embryos is extracted by PicoPure RNA Isolation Kit. Double-stranded cDNA was then synthesized by SMARTer Pico PCR cDNA Synthesis Kit and Advantage 2 PCR Kit, and purified by AMPure XP beads. After fragmentation with a Bioruptor Sonication System, cDNA libraries were generated by VAHTS Universal V6 RNA-seq Library Prep Kit.