GSM5710148: V5 ChIP in OSKM expressing, Dox OFF G1297; Mus musculus; ChIP-Seq
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibodies; anti-V5 (Abcam, rabbit polyclonal, ab15828) and anti-OCT4 (CST, rabbit polyclonal, #2750). Libraries were prepared using ThruPLEX DNA-Seq Kit (TaKaRa) according to the manufacturer's instruction.