GSM5903005: t10_control; Pseudomonas aeruginosa; OTHER
Total RNA was extracted using hot-phenol extraction, followed by DNAse treatment. The enriched samples were subjected to an enrichment procedure based on streptavidin beads to enrich for primary transcripts. For the control samples the enrichment steps were omitted. Total RNA was enriched for primary transcripts using an adapted version of the (SMRT)-cappable-seq enrichment protocol. In parallel, control samples were not enriched but subjected to similar environmental conditions. The RNA was reverse transcribed, PCR amplified and barcoded according to Oxford Nanopore Technology cDNA-PCR protocol (SQK-PCS109 combined with SQK-PBK004). The amplified cDNA samples were pooled together in a final library and loaded on a promethION flowcell.