GSM4909200: Lung Ricin 8h scRNA-seq; Mus musculus; RNA-Seq
Lungs were removed, lyses into a single cells. The protoplast suspension was loaded into Chromium microfluidic chips with 3' (V3) chemistry and barcoded with a 10× Chromium Controller (10X Genomics). RNA from the barcoded cells was subsequently reverse-transcribed and sequencing libraries constructed with reagents from a Chromium Single Cell 3' v3 reagent kit (10X Genomics) according to the manufacturer's instructions.