GSM4844575: C-20h-resting-rep1; Homo sapiens; RNA-Seq
Total RNA was extracted from eosinophils using the RNeasy Mini Kit (Qiagen, Valencia, CA) and treated with DNase (RNase-free DNase kit, Qiagen). RNA samples were submitted to the University of Wisconsin-Madison Biotechnology Center (Madison, WI) for RNA quality and integrity evaluation via Agilent 2100 Bioanalyzer platform (Agilent Technologies), library preparation and sequencing. Takara SMARTer v4 Ultra-Low Input RNA / NexteraXT Library Prep