GSM5017937: 5OP-TNF_rep2; Homo sapiens; RNA-Seq
Cells were sorted and RNA extracted using RNAqueous™-Micro Total RNA Isolation Kit according to the manufacturer's instructions. DNase treatment of RNA was performed using TURBO DNA-free™ Kit (Invitrogen). Samples were quantified and quality checked on an Agilent Bioanalyzer 2100 library prepared using Ovation® SoLo RNA-Seq System (Human) (NuGEN)