GSM5808541: WTP8rep1; Mus musculus; RNA-Seq
RNAseq: Retinas were dissected out from P4 or P11 Brn3b-Kir2.1 or Brn3b-cre mice. Isolated retinas were enzymatically dissociated in a mixture of collagenase/Trypsin and BSA for 20 minutes at 37.C followed by mechanical dissociation. Total RNA extraction was performed with Arcturus. Picopure. RNA isolation Kit (ThermoFisher Scientific). The samples were sequenced according to the manufacturer instructions in a HiSeq Sequencing v4 Chemistry (Illumina, Inc). RNAseq samples were sent for library preparation and NGS service to Fasteris SA (Switzerland).