GSM4910463: Gut bacteria of hooded crane in the early period-61 [Paired-end]; gut metagenome; OTHER
Faecal DNA was extracted using the faecal DNA extraction kit (QIAamp Fast DNA Stool Mini Kit, MP Biomedicals, Santa Ana, CA, USA), quantified by micro-spectrophotometer (NanoDrop ND-1000, Thermo Fisher Scientific, Waltham, MA, USA) and stored at -20 °C. Purified DNA (30 μl) was extracted from each sample as an amplification template and sent to the Illumina Mi-seq platform of Shanghai Meiji Biological Company. The V4-V5 hypervariable region of the bacterial 16S rRNA gene fragment was amplified with primers F515/R907. Forward Primer: 515F（5'-GTGCCAGCMGCCGCGG-3'）;  Reverse Primer: 907R（5'-GGACTACHVGGGTWTCTAAT-3'）