GSM5768857: PD_1_Spleen_1  [miRNA-Seq]; Mus musculus; miRNA-Seq
RNA was harvested using Trizol reagent. A total amount of 3 μg total RNA per sample was used as input material for the small RNA library. Sequencing libraries were generated using NEBNext®Multiplex Small RNA Library Prep Set for  Illumina®(NEB, USA.) following manufacturer's recommendations and index codes were added to attribute  sequences to each sample.