GSM6042915: Young skin; Heterocephalus glaber; RNA-Seq
Total RNA was extracted from full-thickness dorsal skin samples obtained from young and old animals and purified using Direct-zol RNA purification kit (Zymo Research; Irvine, CA) following by DNAse treatment. Integrity of RNA was assessed on 2100 Bioanalyzer Instrument (Agilent Technologies, Inc.), only samples with RIN factor above 7.5 were used for NGS library preparation. For RNA sequencing, approximately 10 ug of total RNA was used to remove ribosomal RNA according to the manuscript of the Epicentre Ribo-Zero Gold Kit (Illumina, San Diego, USA). The pair-end 2×150bp sequencing was performed on an Illumina Hiseq 4000 platform in the LC Sciences (Houston, TX).