GSM5493234: ATAC MEL parental rep2; Mus musculus; ATAC-seq
ATAC-seq was performed as previously described (Buenrostro et al., 2015). Briefly, 50,000 cells were washed and lysed using cold lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2 and 0.1% IGEPAL CA-630). The pellet was then resuspended in the transposition reaction mix (25 μL 2× TD buffer, 2.5 μL Tn5 Transposes (Illumina) and 22.5 μL nuclease-free water) and purified using a Qiagen MinElute Kit. Transposed DNA fragments were amplified by PCR and libraries were sequenced on Illumina HiSeq 2500 with paired-end reads.