GSM5019737: Brain WT sedentary - rep 4; Mus musculus; RNA-Seq
The right hemisphere was cut into small cubicles and incubated at RT for 20 min in RNAlater (Qiagen, Hilden, Germany) before being stored at -80°C. NGS library preparation was performed with Illumina's TruSeq stranded mRNA LT Sample Prep Kit following Illumina's standard protocol (Part # 15031047 Rev, E). The libraries were prepared with a starting amount of 302 ng and amplified in 12 PCR cycles, profiled in a DNA 1000 Chip on a 2100 Bioanalyzer (Agilent Technologies) and quantified using the Qubit dsDNA HS Assay Kit, in a Qubit 2,0 Fluorometer (Life technologies). All 24 samples were pooled in equimolar ratio and sequenced on 1 NextSeq 500 Highoutput FC, PE for 2x 42 cycles plus 7 cycles for the index read.