GSM4974558: Ldlr KO 2 RNA-seq; Mus musculus; RNA-Seq
For RNA sequencing the RNeasy micro kit (Cat. No. 74004, Qiagen) as per manufacturers protocol. For single cell sequencing the 10x Genomics Single Cell 3′ v2/v3 Reagent Kit user guide for the non-BMT (Ldlr-/-) and BMT (WT to Ldlr-/-) sample respectively. For RNA sequencing the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina®, using the manufacturer's direct RNA input protocol (NEB). For single cell sequencing the v2 Chromium™ Single Cell 3ʹ Library Kit and the v3 Chromium™ Single Cell 3ʹ Library Kit was used for the non-BMT (Ldlr-/-) and BMT (WT to Ldlr-/-) sample respectively.