GSM6042698: Ptpn6+/mev 3, IRI,24h; Mus musculus; RNA-Seq
Kidneys were removed, flash frozen on dry ice. RNA was harvested using Rneasy mini plus kit (Qiagen). 1 μg of total RNA was enriched for the Poly-A mRNA and reverse transcribed to double-stranded cDNA. The cDNA was end repaired, adenylated, and ligated with Illumina indexes prior to 12 cycles of PCR.