GSM4910066: KM12SM input DNA; Homo sapiens; ChIP-Seq
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP libraries were prepared in BGI Genomics (Wuhan, China) following manufacturer protocols. Briefly, ChIP-DNA was end-repaired, 3'-dA overhang was added and methylated sequencing adaptor was ligated. The libraries were amplified, followed by an extra step of size selection. A range of fragment sizes between 100-300bp (including adaptor sequence) was selected for loading onto the flow cell. Libraries were sequenced in single-read mode for 50 bases on the BGI-500 sequencer.