GSM5870057: D5_stage2; Homo sapiens; RNA-Seq
We extracted RNA using the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen, Netherlands, Cat. No. 80224) following the manufactures´ extraction protocol. Libraries were prepared following the TrueSeq stranded protocol from Illumina (California, USA). We sequenced the RNA by the HiSeq 4000 (Illumina) with a paired-end 100 run like performed in the publication of M. Awwad et al. (Selective elimination of immunosuppressive T cells in patients with multiple myeloma.Leukemia (2021). https://doi.org/10.1038/s41375-021-01172-x)