GSM4910240: Intestinal Enteroid 56hr (Hs); Homo sapiens; RNA-Seq
At each collection timepoint, cells were pelleted, suspended in 1mL TRI reagent (Molecular Research Center) and snap frozen in liquid N2. After completion of the time course, RNA was isolated via a RNeasy mini-kit column (Qiagen). The cDNA synthesis and amplification were done as recommended by Clontech. cDNA libraries were prepared using Nextera XT DNA Sample preparation reagents. Fragmented and tagged libraries were quantified using an agilent bio-analyzer, pooled and sequenced on a HiSeq 2500 platform (Illumina) using the pair-end 100-bp sequencing strategy.