GSM5492196: Spleen_combi; Mus musculus; RNA-Seq
Spleens/tumours were collected from mice 25 days following tumour implantation. Following treatment, spleens and tumours were collected, processed to single-cell suspension via mechanical and enzymatic disruption of tissue, and samples from the same experimental group pooled together for each tissue type. P1A-35-43-specific CD8+ T-cells were then isolated from spleens and tumours by MHC-class I multimer staining and FACS sorting and resuspended in PBS-FCS. Cells were then loaded onto the 10X Genomics Chromium system as per manufacturer's instructions. Library construction was performed according to the 10X Chromium Single Cell 5' Reagent Kit Single cell libraries were constructed with the Single Cell 5' Reagent Kit from 10X genomics