GSM6189432: T0 rep3; Saccharomyces cerevisiae; RNA-Seq
RNA was extracted using hot acid phenol, followed by ethanol precipitation, and suspension in Milli-Q water 500 ng of total RNA were treated with Baseline-ZERO DNAse (Epicentre) prior to ribosomal RNA depletion with the Ribo-Zero Yeast magnetic Kit (Illumina). RNA libraries for RNA-seq were prepared using the Stranded Total RNA Preparation kit following manufacturer's protocols.