GSM5492317: CD8 395; Mus musculus; RNA-Seq
Total RNA was obtained from either CD4+ or CD8+ FACS-sorted cells by disrupting samples in Trizol. Following phenol chloroform extraction, RNA integrity was quantified on a Nanodrop Spectrophotometer and samples with A260/280 > 1.8 utilized for RNA sequencing. Indexed libraries were prepared from 500 ng/ea purified RNA with the TruSeq Stranded mRNA Sample Prep Kit (Illumina) according to the manufacturer's instructions