GSM4839800: VZA01702R.H08; Homo sapiens; RNA-Seq
Individual live cells were FACS-sorted into 384-well plates with lysis buffer, snap-frozen and stored at -80 degrees C. After splitting each cell, separate mRNA- (*R suffix) and DNA-libraries (*D suffix) were constructed. As comparison, a set of cells had mRNA libraries prepared with Smart-Seq2 (no suffix). Barcoded libraries were sequenced paired-end (37bp x 2) on an Illumina NextSeq.