GSM4974177: AT6-2; Homo sapiens; ATAC-seq
Sample preparation was conducted as previously described by Buenrostro et al, with modifications described by Lara-Astiaso and colleges Two sequential 9-cycle and 5-cycle PCR were performed in order to enrich small tagmentated DNA fragments. Libraries were prepared using KAPA HiFi HotStart ready mix. After the first PCR, the libraries were selected for small fragments using SPRI cleanup (0.65x). Then a second PCR was performed with the same conditions in order to obtain the final library.