GSM6257278: HEK293 Slow Pol II Nanopore; Homo sapiens; RNA-Seq
Cytoplasmic RNA of HEK293 cells were achieved by RLN (Cyto-Buffer) and the Reliaprep™ miRNA Cell and Tissue Miniprep System (Promega). mRNAs were isolated by the NEBNext® Poly(A) mRNA Magnetic Isolation Module (E7490) with use of 30 µg RNA. RNA libraries were prepared for sequencing using standard Nanopore protocols for Direct RNA sequencing (SQK-RNA002).