GSM5493444: H2Aub-ChIP H9 (naïve) 7d+EZH2i rep2; Homo sapiens; ChIP-Seq
During harvesting, mouse feeder removal microbeads (Milteny Biotech; 130-095-531) were used to reduce the amount of MEFs in the naive cell samples, according to the manufacturer's protocol. Primed cells were dissociated and centrifuged directly.Three replicates of 1e6 cells per condition were collected and pellets were flash frozen. ChIP-seq libraries were prepared using the MINUTE-ChIP protocol as described in Kumar & Elsässer 2019; PMID: 3153304 and sequenced on the Illumina NextSeq 500 (Paired End) platform. Antibodies used : H2AUb Cell signalling 8240S; lot #6 , H3K27me3 Millipore 07-449; lot #3091919 , H3K4me3 Millipore 04-745; lot #3198414.