GSM4973281: USA400 MW2 Planktonic 5 hour; Staphylococcus aureus; RNA-Seq
Total bacterial RNA was isolated utilizing an RNeasy Kit (Qiagen) and contaminating DNA was removed by treatment with TURBO DNA-free kit (Ambion). Samples with a RIN of >9.7 measured by Agilent 2100 Bioanalyzer system and corresponding RNA 6000 Nano kit (Agilent) were used in this study. Ribosomal RNA was removed using a Ribo-Zero Kit for Gram-positive Bacteria (Illumina) and MICROBExpress Bacterial mRNA enrichment kit (Agilent). Library construction was performed using the TruSeq Stranded mRNA Kit from Illumina omitting mRNA enrichment steps.