GSM6043131: CD_F6; Mus musculus; RNA-Seq
RNA was extracted using TRIzol reagent and further purified/DNase treated with the RNeasy Kit cleanup protocol (Qiagen, Hilden, Germany). 300 ng of total RNA were used for the construction of the sequencing libraries. Libraries were prepared using the TruSeq Stranded Total RNA Library Prep kit  (Human/Mouse/Rat 96 samples ref. 20020597, Illumina) according to the manufacturer's protocol, to convert total RNA into a library of template molecules of known strand origin and suitable for subsequent cluster generation and DNA sequencing.