GSM5596814: Hepatic endothelial cells - Alk1-HEC-KO No4; Mus musculus; RNA-Seq
Non-parenchymal liver cells were separated by a 19.3 % Nycodenz (1002424, Axis-Shield, Alere Technologies) gradient. Then, magnetic-activated cell sorting (MACS) was used with anti-CD146 MicroBeads (ME-9F1, 130-092-007, Miltenyi Biotech) to purify endothelial cells. RNA from hepatic endothelial cells was isolated using the innuPREP RNA Mini Kit 2.0 (845-KS-2040250, Analytik Jena). RNA libraries were prepared for sequencing using standard BGISeq-500 protocols.