GSM4975191: RNA-seq of HeLa Kyoto cells treated with JAZF1 siRNA pool, 1. Replicate, Batch one; Homo sapiens; RNA-Seq
Total RNA was isolated using the RNeasy Mini Kit (QIAGEN) and on-column DNase digestion (RNase-Free DNase Set, QIAGEN) was performed according to the manufacturer's instructions. RNA libraries were generated with QuantSeq 3' mRNA-Seq Library Prep Kit FWD (Lexogen) according to the manufacturer's instructions. RNA-seq (75 bp read length, single-read) on the Illumina NextSeq550 platform