GSM5866832: D28_WT_KIDNEY_ORGANOID_HNF1A_CHIP; Homo sapiens; ChIP-Seq
Nuclear lysates were clarified from sonicated kidney organoids following dissociation using TrypLE. Immunoprecipitation of HNF1A protein was performed using HNF1A antibody (ab96777). Library preparation was done using a commercially available kit, NEB# E7645 NEBNext Ultra II DNA Library Prep Kit for Illumina KIT® following the manufacturer's protocol. During the library construction, the ChIP-ed DNA underwent End Repair, Adapter ligation, size selection (for ~150bp insert)  and 16 cycles PCR enrichment to generate the final sequencing ready library. The quality of the library was checked using Agilent D1000 ScreenTape. The final library should have a fragment size of ~270bp insert plus adaptor and PCR primer sequences). The quality of the library was checked using Agilent D1000 ScreenTape. A single peak was expected and was observed indicating that the library was good and suitable for sequencing.