GSM4973760: input_1; Homo sapiens; ChIP-Seq
10x10e6 cells were cross-linked with 1% formaldehyde and quenched for 5 min in 1 M Glycine. After centrifugation, cell pellets were resuspended in lysis buffer (1% SDS, 50 mM Tris-HCl pH8, 10 mM EDTA). Sonication was performed with Ultrasonicator (Covaris). Lysates from 2.5 x106 cells were processed with the iDEAL ChIP kit (Diagenode) according to the manufacturer's recommendations using 5 μg of antibody (ERRα: GTX108166, Genetex). Quality of samples was assessed on the Agilent TapeStation 4150 using D5000 ScreenTape and Agilent TapeStation Software 3.2. Libraries were prepared according to Illumina's instructions. The ChIP DNA libraries were sequenced as paired-ended 81bp reads with an Illumina Next Seq 500 sequencer following the manufacturer's protocols. Sequencing gave 29.106 and 10.106 reads for ERRα and input samples respectively.