GSM5060863: Gill tissue- normoxia [CON _1]; Tachysurus vachellii; RNA-Seq
Total RNA of gill tissues (n=9 per group) sampled at 96h was extracted by using TRIzol reagent (Invitrogen, USA). The RNA integrity and concentration were monitored by Agilent Bioanalyser 2100 (Agilent Technologies, USA) and NanoDrop Lite spectrophotometer (NanoDrop Technologies, USA). Then, equal amounts of extracted RNA from each group (hypoxia group, control group) were mixed (every three samples) for sequencing. About 10 μg total RNA from these mixed sample was purified by using oligo-dT magnetic beads and then prepared for constructing RNA-seq libraries by mRNA-Seq Sample Preparation Kit (Illumina, USA) (Qiang et al., 2017). Transcriptome sequencing was performed by using Illumina Novaseq™ 6000 (LC Sciences, USA) that generated about 150bp double-ended (PE150) raw reads.