GSM5060255: E15 Ebf2-EGFP pos cells rep1; Mus musculus; RNA-Seq
Ebf2-EGFP positive and negative cells at E11.5, E13.5, E15.5 were dissociated, resuspended and purified by FACS. Total RNA was extracted using QIAGEN RNeasy Mini Kits and processed by Sera-Mag Oligo (dT) beads (Thermo Scientific), libraries were prepared using the NEB Next mRNA Sample PreP Master Mix Set 1. RNA libraries were prepared using the NEB Next mRNA Sample PreP Master Mix Set 1 and were performed according to the manufacturer's instruction.