GSM4909946: Ventral Fusion Region Day 90; Homo sapiens; RNA-Seq
Organoid Fusions were first manually dissected. Tissue was then dissociated by a 30min incubation at 37 oC  at 800rpm shaking speed on a ThermoMixer® . GFP+ cells were then obatiend via FACS. Libraries were generated with the 10x Chromium Single cell 3' GEM, Library & gel Bead Kit v3 (10x Genomics, cat. #PN-1000075). Libraries were pooled and sequenced on a NovaSeq S1 flow cell (Illumina) with 2.6-3.2 billion paired-end reads.