GSM5060330: CK_rep3; Mus musculus; RNA-Seq
RNA was extracted using Trizol reagent. mRNA was enriched with oligo(dT)-attach magnetic beads and fragmented into small pieces, cDNA library was generated using random hexamer-primed reverse transcription followed by second-strand cDNA synthesis and sequenced on an MGI 2000 platform (BGI, Shenzhen, China). RNA libraries were prepared for sequencing using standard BGI 2000 protocols