GSM5328329: 2ME3; Xenopus tropicalis; RNA-Seq
For 0hpa, ~250 um of tissue was collected anterior to the amputation plane. For 24hpa, the tadpoles were anestetized and ~250 microns of tissue was collected. RNA was extracted via phenol:chloroform extraction following by ethanol precipitation and DNAseI treatment. RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following manufacturer's instructions.