GSM4972190: 1M7_modified_Run2; Mus musculus; OTHER
The libraries were constructured using the SHAPE-MaP small RNA workflow  protocol (Smola et al., 2015) with minor alternations. The eRNAs were cleaned up after chemical treatment with 1.8x AmpureXP beads  (Beckman Coulter) according to the manufacturer's instructions. The modified eRNA was subjected to reverse transcription using a specific SHAPE-MaP RT primer complementary to the 3' linker region. The produced cDNA was cleaned up with 1.8x AmpureXP beads and was amplified in a first PCR using partial Illumina primer and second PCR using the RP1 and two different Index-primers (Illumina)for DMSO control and the 1M7 modified libraries. The DNA from the 1stPCR was purified with 1.0x AmpureXP beads and from the 2nd with 0.8x. Final libraries were checked on a fragment analyzer and pooled equimolar before sequencing.