GSM5965466: CLP Heart Vehicle rep2 [A17-Heart]; Mus musculus; RNA-Seq
Experimental peritonitis was induced in 7- to 12-week-old male mice by either  cecal ligation and punction (CLP model)  or injecting i.p. with 1 mL of Brewer thioglycollate medium (TGC, 4% in distillated water) (TGC model). Administration of β-NMN (185 mg/kg/day) and vehicle (saline, 0.9% NaCl) were performed immediately after peritonitis induction, followed by daily injections for up to 4 days before euthanasia and the last treatment was 24 hours before sacrifices. For heart and lungs, 10 mg of tissues were treated with RNAlater solution. In TGC model, cells from peritoneal lavage were plated at 0.5x10 6 cells/well in HBSS+/+ and stimulated during 6 hours at 37°C, 5% CO 2 , with E.coli (7.5x10 6 bacteria; MOI of 1:15). Supernatants were discarded and cells were treated with 100 µL of RNAlater solution. RNA libraries were prepared for sequencing using standard Illumina protocols