GSM4910672: RNAseq.dom-kd.st5.rep3; Drosophila melanogaster; RNA-Seq
RNA extraction was performed as in Zenk et al., 2017. Briefly, chloroform extraction was performed followed RNA precipitation with Isopropanol. Samples were then washed twice with 80% EtOH and resuspended in TURBO DNase (Ambion AM2238) reaction mix. Finally, DNase was inactivated. RNA libraries were prepared using the Illumina TruSeq Stranded Total RNA protocol