GSM5866575: Mist1_MTMG3; Mus musculus; RNA-Seq
Human epithelial cell suspensions were obtained by EDTA chelation and cold protease dissociation. Cells were encapsulated using the inDrop platform (1CellBio), and RNA was extracted according to the protocol of Klein et al., 2015. MARIS libraries are generated according to Chen et al., 2021. CEL-Seq linear amplification of using standard structure (single index) (Klein et al., 2015) or TruDrop library structure (dual index) (Southard-Smith et al., 2020) RNA-Seq was performed on Novaseq6000 with a 2 x 150 paired-end kit standard run (for dual index) RNA-Seq was performed on NextSeq500 with a 2 x 75 paired-end kit using custom read lengths (for single index)