GSM5709954: DM-NP; Rattus norvegicus; RNA-Seq
Buccal and lingual gingiva tissue around the maxillary left second molars were removed minced on ice to less than 1 mm cubic pieces. They were digested by collagenase I (2mg/mL, GIBCO), collagenase II (2 mg/mL, GIBCO) and trypsin-EDTA (0.25%, GIBCO) at 37℃ for 60 min. Cell suspension was then filtered through a 40-μm strainer (ThermoFisher Scientific), and the supernatant was removed after centrifugation. RNA libraries were prepared with Chromium Single Cell 3' Reagent v3 Kits according to to the manufacture's protocol.