GSM6042945: BG1; Homo sapiens; miRNA-Seq
hADSC and BG-primed hADSC CM were concentrated using Vivaspin 20 centrifugal concentrator at 2,000 g at 4 °C. Then, the concentrated supernatant solution (around 200 μL left in the concentrator) was collected and added with Exo-Prep solution in a ratio of 1:1. The mixed solution was incubated on ice for 1 hour, then the solution was centrifuged for 20 minutes at 10,000 g at 4 °C to obtain EV pellets.