GSM4972323: Sample_09-00410_T; Homo sapiens; RNA-Seq
A cDNA library, consisting of 151 bp long fragments, was constructed for paired-end sequencing using the TruSeq stranded mRNA Sample Preparation Kit (Illumina, CA, USA), according to the manufacturer's instructions. Briefly, mRNA was purified from 1 μg of total RNA, which was extracted from the tissue samples, and fragmented using oligo (dT) magnetic beads. Single-stranded cDNA was synthesized from the fragmented mRNA through random hexamer priming. The single-stranded cDNA is then used as a template for the synthesis of the second strand in order to construct double-stranded cDNA molecules. After end repair, A-tailing, and adapter ligation, cDNA libraries were amplified using Polymerase Chain Reaction (PCR). The quality of the cDNA libraries was evaluated with the 2100 BioAnalyzer