GSM6257244: Human brain organoids, CHO; Homo sapiens; RNA-Seq
To obtain a single-cell suspension,  5-7 organoids at D30 were randomly selected into a 1.5 ml EP tube with 1 ml of TrypLE (Life Technologies) and incubated in a 37 degrees Celsius incubator for 40 min with gentle agitation every 5-8 min. The organoids were then washed with DPBS (Life Technologies) 3 times and gently dissociated into single cells with a 200 microliter pipette. The organoids were dissociated by trypLE (Life technology) into single cells. The cell debris and other aggregates were removed by an additional low-speed centrifugation. About 5,000 cells were captured and the released RNA were barcoded through reverse transcription in individual GEMs. Droplet-based single-cell RNA sequencing was performed using the 10X Genomics Chromium Single Cell 3 platform, version 3, according to manufacturers instructions.