Illumina HiSeq 2000 sequencing; Whole transcriptome profiling of beta cell development in the mouse
Total RNA was isolated using TRIzol LS (Invitrogen) and column purification (Zymo Research), according to manufacturers instructions. RNA was DNase-treated (Ambion) and integrity was assessed on the Bioanalyzer (Agilent). See file RNA_extraction_for_FACS.doc. As per manufacturer instructions. The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008).