GSM5811627: spinal cord, 1 week post transection 1 (RNA-seq); Danio rerio; RNA-Seq
Sham injured and injured (2 mm rostral and caudal to the lesion site) spinal cords were dissected and RNA was harvested using Trizol reagent. Genomic DNA was removed using RNA Clean and Concentrator Kit (Zymo Research).  Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using Hiseq4000 (50bp, SR, 1 lane)