GSM6042769: P2x1-KO; Mus musculus; RNA-Seq
Wild Type (P2X1-Scr) and P2x1 knockout (P2X1-KD)  AML leukemia leukemia-initiating cells  were lysed by TRIzol reagent for extraction of RNA. RNA was prepared using RNasey Mini Kit(Qiagen p/n 74104) according to  the manufacturer's recommendations. After clean-up, RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored by RNA electrophoresis. RNA libraries were prepared for sequencing using standard Illumina protocols