GSM5965693: JFH1-SGR-Feo_4hrs_Rpp_rep2; Homo sapiens; OTHER
RNA extraction was performed by Trizol treatment followed on-column DNase I digestion and RNA purification using RNA Clean & Concentrator kit-5 RNA was enzymatically treated with de-capping enzymes that specifically convert different 5' cap structures to 5' monophosphates. 5'-phosphate dependent adapter ligation was performed followed by reverse transcription. Libraries were generated using PCR with custom Illumina compatible primers.