GSM4972508: Zebrafish Intestine - CV; Danio rerio; RNA-Seq
Zebrafish from each feeding group were fasted for 24 hours. Zebrafish were euthanized with 0.4% MS-222. Intestines were isolated and immersed in RNALater until extraction of RNA. Total RNA was isolated using GeneJET RNA Purification kit according to manufacturer's protocol (ThermoFisher Scientific). 1µg of total RNA with RIN values above 7 were used for library preparation. Poly(A) mRNA isolation was performed using NEBNext Poly(A) mRNA Magnetic Isolation Module. NGS library preparation were constructed with NEBNext Ultra RNA Library Prep Kit for Illumina