GSM6042747: Wrinkly Fried Egg + Acanthamoeba Rep 4; Pseudomonas fluorescens; Acanthamoeba sp.; RNA-Seq
Total RNA was extracted from bacterial and amoebae cells using the Quick-RNA MiniPrep Kit (Zymo Research, USA) Preparations were dried in GenTegra RNA tubes according to manufacturer's instructions (Gentegra, USA) and sent to the Microbial Genome Sequencing (MiGS, USA) Center for Illumina Stranded RNA library preparation with RiboZero Plus rRNA depletion and sequencing on the NextSeq 550 platform.