GSM6190594: AB183_24H_R3; Homo sapiens; RNA-Seq
Cells were harvested according to the protocol of the Quiagen RNAeasy Mini Kit. Total RNA was isolated by using the RNeasy Mini Kit (Qiagen) with on-column DNase I digestion. mRNA was isolated using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). cDNA libraries were generated with purified mRNA by using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs). Libraries were size selected using Agentcourt AMPure XP beads, followed by amplification with 12 PCR cycles. The quality of the library was determined with the Fragment Analyzer (Advanced Analytical). Libraries were sequenced on the NextSeq500 platform (Illumina).