GSM4909020: HT1376 sh5 (TRAF4)_D; Homo sapiens; RNA-Seq
Total RNA from cells was isolated using the NucleoSpin RNA II kit (740955, BIOKE) using the manufacturer's instructions. Thereafter, RNA samples were processed for transcriptomic sequencing and analysis. BGISEQ-500 RNA-Seq Library protocol (SOP-SS-027) was followed. mRNA molecules were purified from total RNA using oligo(dT)-attached magnetic beads. mRNA molecules were fragmented into small pieces using fragmentation reagent after reaction a certain period in proper temperture. First-strand cDNA was generated using random hexamer-primed reverse transcription, followed by a second-strand cDNA synthesis. The synthesized cDNA was subjected to end-repair and then was 3' adenylated. Adapters were ligated to the ends of these 3' adenylated cDNA fragments. PCR process was applied to amplify the cDNA fragments with adapters from previous step. PCR products were purified with Ampure XP Beads (AGENCOURT), and dissolved in EB solution. Library was validated on the Agilent Technologies 2100 bioanalyzer. DNBSEQ- RNASeq-PolyA – BGI index