GSM5710411: Al-WT-1; Arabidopsis thaliana; RNA-Seq
Total RNA was isolated using a RNeasy Mini Kit (Qiagen). First strand cDNA was generated using reverse transcriptase and random primers. The constructed cDNA libraries was qualified and quantified with Agilent 2100 Bioanaylzer and ABI StepOnePlus Real-Time PCR System, and then sequenced via Illumina HiSeqTM 2000 or other sequencer when necessary at the Beijing Genomics Institute, Shenzhen, China.