GSM5596930: S196R_Striatum; Mus musculus; RNA-Seq
Immediately following euthanasia, the mouse brains were harvested and the front right or left quadrants were excised, placed in RNAlater (Qiagen), and stored at -80 °C. RNA extraction was performed using the RNeasy Mini Kit (Qiagen). mRNA was isolated using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Ipswich, Massachusetts). Library preparation was performed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Sequencing was performed using a NextSeq 500 (Illumina, San Diego, California) at a target depth of 25 million 2 x 75 bp paired end reads per sample.