GSM6042982: FC687, replicate 2, scRNAseq; Homo sapiens; RNA-Seq
RNA samples of mononuclear/polymorphnuclear cells are harvested by Tri-zol (Invritrogen, Inc. Faraday Avenue, Carlsbad, CA). (500 μl Tri-zol/600 μl cell lyses buffer for each 1 × 105 cell) Single-cell RNA-Seq libraries were prepared using GemCode Single-Cell 3' Gel Bead and Library Kit (now sold as P/N 120262, 1000009, 120267, 10x Genomics). GEM-RT was performed in a Veriti 96-Well Thermal Cycler 2020/7/29 Version1 (Applied Biosystems; Model#: 9902)at 53 °C for 45 min, 85 °C for 5 min, and held at 4 °C.