GSM5491702: Lut_axenic3; Luteibacter sp. 9143a; RNA-Seq
Samples were ground in TRIzol, then chlorofom and ethanol were used to isolate nucleic acids. The samples were treated with DNAse and checked for quality and contamination. From RNA samples from fungi-containing cultures, fungal rRNA was depleted twice and poly-A selected to acquire fungal transcripts. The remaining RNA from the coculture sample and the RNA from the axenic bacteria sample were depleted twice of bacterial rRNA. Sequencing libraries were prepared using RNA TruSeq library construction kits.