GSM6185906: D5_100_M,Replicate1; Mus musculus; ATAC-seq
Crosslinked and TF-Sorted ATAC-seq (CaTS-ATAC) was performed as described in the associated manuscript. In brief, cells were PFA fixed, subject to Tn5 transposition for ATAC-seq, stained with intracellular antibodies and flow sorted. After overnight reverse crosslinking, DNA was extracted using Zymo DNA concentrator kit. Transposed DNA was first amplified as described in Corces et al (2017) and Ma et al (2020)