GSM5904409: WGC-12d-4; Danio rerio; RNA-Seq
Gonads were dissected, Germ cells were sorted by FACS. and directly into lysis buffer provided with the SMART-seq HT Kit (Takara, Cat. 634438). The SMART-seq HT Kit was then used for reverse transcription and cDNA amplification.  In SMART-seq HT, full-length cDNA was synthesized in one step, and then amplified by 12 cycles of PCR. After purification with the AMPure XP kit (Beckman Coulter), cDNAs were analyzed by a bioanalyzer for their qualities. cDNA libraries were generated using the SMART-seq HT Kit (Takara, Cat. 634438). Final libraries were prepared using Nextera XT DNA Library Prep Kit (Illumina, FC-131-1096).