GSM5060919: Ci4_F1_E11; Homo sapiens; RNA-Seq
Cells were sorted into 96 well plate with a flow cytometer SH800 (SONY) and dissolved with single cell lysis buffer (0.5% NP40). Whole-transcript amplification was performed as previously described (Sasagawa et al. 2013; Sasagawa et al. 2017). Amplified cDNA from single cell was processed for library preparation using Nextera XT Library Prep Kit (Illumina).