GSM5902777: A549_siCtrl_3rd; Homo sapiens; RNA-Seq
Total RNA was extracted with TRIZOL (Ambion).  RQ1 DNase (Promega) was used to remove any remaining DNA form the total RNA.  RNA quality and quantity were assessed by measuring the absorbance at 260nm/280nm (A260/A280) using Nanodrop One (Thermo).  RNA integrity was further verified by electrophoresis through a 1.5% agarose gel. After ligation, the cDNAs were fragmented into 300–500bp fragments. Finally, the DNA fragments were amplified, purified, quantified, and stored at -80℃ until used for sequencing. The dUTP marked second strand was not amplified, allowing strand specificity.