GSM5493258: ESC Matr3KO CTCF rep1; Mus musculus; ChIP-Seq
Cells were crosslinked and nuclei were prepared using truChIP Chromatin Shearing Reagent Kit (Covaris). Purified ChIP DNA was processed to generate libraries using the NEBNext ChIP-seq Library Prep Master Mix, NEBNext Ultra DNA Library Prep Kit for Illumina (New England BioLabs), ThruPLEX DNA-Seq Kit (Rubicon Genomics), or Accel-NGS 2S Plus DNA Library Kit (Swift Biosciences) according to the manufacturer's instructions.