GSM5021131: PTBP1.100K.K562_KD.rep2; Homo sapiens; RIP-Seq
After UV-C irradiation, RNA cross-linked to protein is purified and ligated with an adenylated DNA linker at the 3'end. Next, cDNA fragments, which stop exactly at the cross-link site in RNA, are generated through reverse transcription (RT), followed by polyadenylation, in vitro transcription (IVT), PCR amplification and high-throughput sequencing.