GSM5060153: WT_16w_01; Mus musculus; RNA-Seq
Total RNA from excised whole bladders was isolated using a NucleoSpin RNA kit (TaKaRa). For RNA sequencing, 500 ng total RNA were rRNA-depleted using the NEBNext rRNA Depletion Kit (New England Biolabs) according to the manufacturer's instructions. Library preparation was performed using an NEBNext Ultra Directional RNA Library Prep Kit (New England Biolabs).