GSM6257613: PSEN1-A79V replicate 3 [RNA-seq]; Homo sapiens; RNA-Seq
Total RNA from magnetically purified human NDC, PSEN1_A79V, PSEN2_N141I, and APP_V717I hiPSC-derived neurons (replicates, n=3) using the RNeasy Plus Micro Kit (QIAGEN, catalog no. 74034) according to the manufacturer's protocol. On-column deoxyribonuclease digestion was performed on total RNA extracts to remove any genomic contamination (QIAGEN, catalog no 79254) Libraries were prepared for RNA-seq using the TruSeq Stranded Total RNA Library Prep Kit (Illumina, catalog no. RS-122-2303) by the Ribo-Zero ribosomal RNA reduction method (Illumina, catalog no. MRZG12324). Samples were sequenced at the UC San Diego Institute for Genomics Medicine (IGM) sequencing core on an Illumina HiSeq 4000 generating paired-end, 100-bp reads with an average of 25 million reads per sample (Illumina, catalog no. FC-410-1001)