GSM6255925: Organoids, Cys247X, D100, Rep2; Homo sapiens; RNA-Seq
Total RNAs were extracted using the NucleoSpin RNA XS kit according to the manufacturer's protocol. RNA yields and quality were determined using a NanoDrop ND-1000 Spectrophotometer and an Agilent 2100 Bioanalyzer. For RPE cells, RNA-seq libraries were constructed from 1 µg of total RNAs using the TruSeq Stranded mRNA Sample Prep (Illumina) and paired-end sequencing of 75 bases length was performed on a HiSeq 4000 system (Illumina). For retinal organoids, RNA-seq libraries were constructed from 1 µg of total RNAs using the NEB Next Ultra II Directional RNA (New England Biolabs) and paired-end sequencing of 100 bases length was performed on a Novaseq system (Illumina).