GSM5061114: Mouse epiblast stem cells - stock starting population; Mus musculus; RNA-Seq
Cells were digested to single cell with accutase for 10-15 minutes, screened, and resuspended in low FBS Phosphate Buffered Saline solution, counted 16 times for cell concentration, viability, and doublet rate, and then diluted to approprate loading concentrations for the 10X Genomics Chromium Controller. scRNA-seq libraries were prepared with 10x Genomics Chromium system using 3' (V3.0 chemistry) GEM beads and i7 Index kit following standard protocol. Libraries were diluted and submitted to Macrogen for sequencing under the library strategy. Libraries were sequenced using paired-end sequencing (150bp Read 1 and 150bp Read 2) with a single sample per lane on the Illumina HiSeq X