GSM5965564: Sample 16_Brain cells - 14_day_Males_rep1; Drosophila melanogaster; RNA-Seq
Pooled brains (100 brains/sample) were homogenized in TRIzol via a bead mill. Total RNA was isolated using the TRIzol reagent protocol (Invitrogen, Carlsbad, CA) and stored at -80°C. RNA quantity and quality were determined with a Qubit 4 Fluorometer and BioAnalyzer 2100 (Agilent Technologies). RNA samples were prepared for RNA sequencing using the TruSeq RNA Library Prep Kit v2 and subsequently sequenced with a NextSeq2000 at the Genomics Core at The Scripps Research Institute, San Diego.