Illumina HiSeq 2500 sequencing; Melanoma-derived extracellular vesicles mediate lymphatic remodeling and impair tumor immunity in draining lymph nodes
Lymph node LECs and medullary sinus macrophages were isolated from inguinal LNs of C57Bl/6N wild type mice. Cells were sorted into 384-well plates containing lysis buffer and RNA was extracted. Libraries were prepared using a miniaturised version of the Smart-seq2 protocol (Picelli et al., 2014).