GSM5904652: Large intestinal stem cell; Mus musculus; RNA-Seq
Lgr5-EGFP+ large intestinal stem cells were enriched from FACS Cellular suspensions were loaded on a Chromium Controller (10x Genomics) to generate single-cell GEMs for the "LISC" sample. Single-cell RNA-Seq libraries were prepared using Chromium Next GEM Single cell 3' Reagent Kit v3.1 following the manufacturer's protocol (PN-1000128, PN-1000127, and PN-220103). scRNA-seq