GSM5492266: LGEs, 2 (CUT&Tag); Mus musculus; OTHER
The cells were isolated from the LGE. Then, 1x10^5 cells were washed with 500 μl of wash buffer (10× wash buffer (-), 50× protease inhibitor cocktail, ddH2O) and centrifuged at 600 rcf for 3 min at RT For library amplification, 24 μl of DNA was mixed with 1 μl of TruePrep Amplify Enzyme (TAE, Vazyme), 10 μl of 5× TruePrep Amplify Enzyme Buffer and 5 μl of ddH2O, and 5 μl of uniquely barcoded i5 and i7 primers from TruePrep Index Kit V2 for Illumina (Vazyme).The libraries were sequenced on an Illumina NovaSeq platform, and 150-bp paired-end reads were generated