GSM4974850: T2 scRNA-seq; Homo sapiens; RNA-Seq
Tumors were resected and dissociated into single cells. To extract circulating tumor cells (CTC), up to 1 ml of blood was drawn immediately post euthanasia using cardiac puncture. Red blood cells were removed using RBC lysis bu_x001B_er. All cells (tumor derived and circulating tumor cells) were stained with DAPI and sorted for DAPI and GFP using a BD FACSAria cell sorter. Libraries were generated using the 10x Chromium single cell gene expression system.