GSM6043301: hTSC, ChIP, H3K4me1; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 12 mins, followed by chromatin extraction and sonication. Immunoprecipitation was performed with 10 µg of chromatin for 2.5 µg of anti-H3K27ac or H3K4me3 antibody. DNA was purified using the GeneJET PCR purification kit (Thermo Fisher). Libraries were prepared from 1-5 ng of DNA using the NEBNext ultra II DNA library prep kit.