GSM6190494: RNA-Seq WT_3h_rep2; Mus musculus; RNA-Seq
B cells were harvested by trypsinization followed by removal of feeder cells through differential adherence to tissue culture dishes for 40 minutes. RNA was extracted from B cells with a miRNeasy mini kit (217004, Qiagen), quantified with a NanoDrop spectrophotometer and its quality examined in a fragment Bioanalyzer (Aligent 2100 Bioanalyzer DNA 7500 assay). For RNA-sequencing (RNA-seq), libraries were prepared with a TruSeq Stranded mRNA Library Preparation Kit (Illumina) followed by single-end sequencing (50 bp) on a HiSeq2500 instrument (Illumina), obtaining at least 40 million reads per sample.