GSM5326660: A5739; Mus musculus; RNA-Seq
RNA was extracted from uninjected mammary fat pad (contralateral to E0771 injected 4th mammary gland) tissue using kit specific for lipid rich tissue (Norgen Biotek, Ontario, Canada).  The integrity of RNA was assessed using Agilent Bioanalyzer and samples with RIN >8.0 were used. Libraries were constructed using NEBNext® Ultra™ RNA Library Prep Kits (non-directional) for Illumina, following manufacturer protocols. mRNA was enriched using oligo-dT beads. Libraries were sequenced on NovaSeq 6000 using paired-end 150 bp reads. There was no PhiX spike-in.