GSM5492408: 9310_EB; Mus musculus; RNA-Seq
VMHvl was manually microdissected and flash frozen in liquid nitrogen. RNA was isolated using the Qiagen Rneasy Micro kit with on-column Dnase digestion. Libraries were prepared using the Tecan/Nugen Trio RNA-Seq Library Preparation Kit (0357-32), using 15 ng of input RNA per sample. Libraries were prepared according to the manufacturer's protocol. Samples were barcoded and multiplexed prior to sequencing.