GSM5021053: E+ 18 hpf sample 1 [sample_9_lane7]; Danio rerio; RNA-Seq
RNA was extracted from embryos staged developmentally at 12, 18 and 24 hpf, pooled (n=10 embryos/sample) and homogenized; 4 sample pools per VitE status. RNA integrity (9.02  0.15, n=16) was assessed using the 2100 BioAnalyzer Instrument (Agilent) at the Center for Genome Research and Biocomputing at Oregon State University. RNA libraries were prepared with the Lexogen QuantSeq 3'mRNA Seq Library Prep Kit-FWD kits (Lexogen, Vienna, Austria). After library preparation, samples were pooled and sequenced using single-end sequencing with 100 bp reads on an Illumina HiSeq3000 instrument (Illumina, San Diego, CA).