GSM5019788: PY-60 treated – Biological replicate 2; Homo sapiens; RNA-Seq
After 24-hour treatment with compound, cells were washed once with PBS and then trypsinized with 500 µL of TrypLE Express dissociation reagent (Gibco) and neutralized with the addition of 500 µL of growth medium. After pelleting cells with centrifugation (300g) for 3 minutes in 1.5 mL microfuge tubes, total RNA was isolated using a RNeasy kit (Qiagen). Three biological replicates were submitted for library preparation and sequencing on an Illumina HiSeq Platform (~58 million paired end reads per sample)