GSM5060225: ChIP-seq Input Ebf2-EGFP E11 pos cells replicate2; Mus musculus; ChIP-Seq
ChIP assays were performed using mouse Ebf2-EGFP positive cells dissociated at E11.5, E13.5, E15.5 and Ebf2-EGFP negative cells at E11.5, with antibodies against H3K27ac (Ab4729, Abcam) and H3K27me3 (Ab6002, Abcam) as previously described (Shen X, et al., 2009). ChIP-Seq libraries were prepared using NEB Next Ultra Ⅱ ChIP-Seq Library Prep Kit (Cat#E7645S & Cat#7335S, NEB) according to the manufacturer's instructions.