GSM5059867: NSG_12; Mus musculus; RNA-Seq
Allografted CT26.WT tumors isolated from mice and total RNA isolated using Qiagen RNeasy mini kit. The integrity of the total RNA was determined using a bioanalyzer (Agilent) and concentration were assessed by Qubit (ThermoFisher Scientific).  A TruSeq RNA kit (Illumina, San Diego, CA) was utilized to prepare the poly(A)-enriched RNA-seq libraries that were sequenced on the Illumina NextSeq 500 in a 75-base single-end mode according to the manufacturer's protocol.