GSM5061617: Bacillus subtilis BJ3-2 45℃; Bacillus subtilis; RNA-Seq
Using TRIzol® Reagent to extract total RNA from tissue samples, Nanodrop2000 detects the concentration and purity of the extracted RNA, agarose gel electrophoresis to detect RNA integrity, and Agilent 2100 to determine the RIN value. A single library construction requires a total amount of RNA of 5ug, concentration ≥200ng/μL, and an OD260/280 between 1.8 and 2.2. The sequencing experiment uses the Illumina TruseqTM RNA sample prep Kit for library construction