GSM5207940: TAD-treated HeLa cells rep1; Homo sapiens; RNA-Seq
For RNA-seq, about 1000,000 cells (top 5% GFP signal) were collected and RNA was extracted according to the standard protocol. For library construction, mRNAs were fragmented and converted to cDNA using random hexamers or oligo(dT) primers. High-throughput mRNA sequencing was carried out  on Illumina NovaSeq 6000 platform with a 150-bp paired-end module.