GSM5061637: RPM_d7_Spic_KO_2; Mus musculus; RNA-Seq
Tissues were removed, cell were FACS sorted and 1000 cells were collected into lysis buffer. oligo-dT primers and dNTPs were added to lysed cells and hybridization of the primer to poly(A) tails PCR products were purified with AMPure XP beads (Beckman Coulter) and 1 ng of the product was tagmented by using the Nextera XT DNA sample preparation kit (Illumina)