GSM5138462: Donor: TSL55 Cell Line: GCTFH; Homo sapiens; RNA-Seq
Cells were sorted directly into RLT lysis buffer with beta mercaptoethanol added and then flash frozen on dry ice. RNA was isolated using Qiagen's RNeasy Micro kit (cat# 74004). 300 pg of total RNA was used to generate cDNA with Takara's SMARTSeq v4 Ultra Low Input RNA Kit for Seuencing (cat# 634892). Illumina's Nextera XT DNA kit (cat# FC-131-1096) was used with 1 ng of unsheared cDNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols on a NextSeq 550 instrument in a Paired-End 75 bp configuration.