GSM5060310: is_129_007; Mus musculus; RNA-Seq
Total RNA was isolated from eWAT (n=5) using the Direct-zol RNA MiniPrep column kit (The Epigenetics Company, Irvine, CA). cDNA libraries were prepared and amplified with the Ion AmpliSeq™ Transcriptome Mouse Gene Expression Kit (Life Technologies, Carlsbad, CA), following the manufacturer`s instructions. Sequencing was performed on an Ion S5™ XL Sequencer (Thermo Scientific, Waltham, MA).