GSM5325531: Dnmt1 CKO  E18.5 lung - strong phenotype MUT_S_265_5; Mus musculus; RNA-Seq
Embryonic E18.5 lungs were homogenized and dissolved in Trizol (Thermo Fisher Scientific). RNA was extracted from the upper aqueous phase with Qiagen RNeasy Mini Spin Columns. In columns-DNAse treatment was performed according to the Qiagen manual. The quality of the purified RNA was confirmed by Agilent Bioanalyzer System, and reverse cDNA library synthesis and sequencing was performed at the Genomic Core at USC (50 M reads, 75 SR per sample). Illumina TruSeq