GSM5020100: Sample Q001S0002B0011R1S; Hordeum vulgare; Blumeria graminis f. sp. hordei; OTHER
Yeast cells were treated with lyticase to digest cell walls and Y2H plasmids were extracted using alkaline lysis and precipitation. Libraries were constructed using the Nugen Ultralow System V2 with Custom AnyDeplete to reduce the abundance of an amplicon common to all libraries using the manufacturer's instructions.