GSM5018469: 102978-001-022; Rattus norvegicus; RNA-Seq
Total RNA was collected using Trizol and the Rneasy Mini Kit. The quality of the RNA samples designated for RNA-Seq was confirmed using the Advanced Analytical Fragment Analyzer. cDNA was generated from total RNA samples with RNA integrity numbers above 7.5 using the NEBNext Ultra Directional RNA library preparation method including polyA enrichment (New England Biolabs, cat# E7420L). Sequencing was performed on a HiSeq 4000 system (Illumina, San Diego, CA). For each sample at least 20 million 150-bp paired-end reads were obtained.