GSM4909967: Liver_BCR; Homo sapiens; RNA-Seq
Viable single cells were prepared from the tissue samples of 15 different organs of a research-consented adult donor. The concentration of single cell suspension was determined using a Cellometer Auto 2000 instrument (Cellometer) and adjusted to 1,000 cells/μl. Approximately 14,000 cells were loaded into a CHROMIUM instrument (10x Genomics, CA, USA) according to the standard protocol of the Chromium single cell V(D)J kit (v1) in order to capture 5,000 ~ 10,000 cells per channel. In brief, mRNA transcripts from each sample were ligated with barcoded indexes at 5'-end and reverse transcribed into cDNA, using GemCode technology (10x Genomics, USA). cDNA libraries including the enriched fragments spanning the full-length V(D)J segments of T cell receptors (TCR) or B cell receptor (BCR), and 5'-end fragments for gene expression were separately constructed, which were subsequently subjected for high-throughput sequencing.