GSM5210612: 20-month brain; Mus musculus; RNA-Seq
Brain tissues were surgically removed and minced into small pieces (approximately 1mm3) on ice and digested by 200 U/mL Papine (Diamond) and Cysteine-HCL (Sigma). Then samples were sieved through 70 µm cell strainers, centrifuged at 300 g for 10 min, and further cleaned for red blood cells using red blood cell lysis buffer (Miltenyi Biotec). RNA libraries were generated by 10xGenomics Single Cell 3' Library Gel Bead Kit V3 and prepared for sequencing using standard Illumina protocols