GSM5020848: 24hr_K3_Unsorted_Rep3; Human betaherpesvirus 5; RNA-Seq
Infected Kasumi-3 cells were collected and lysed in Trizol. Total RNA was prepared using the Direct-zol RNA miniprep kit (Zymo Research) with an on-column DNase digestion step according to the manufacturer's instructions. RNA was rRNA depleted with Ribo-zero (Illumina) and RNA-seq libraries were prepared with TrueSeq Stranded mRNA (Illumina). RNA-seq libraries were sequenced on the Illumina NextSeq 500 Sequencing System (50 bp single-end reads, 50 million reads/sample)