GSM5903769: E14Tg2a_ctcfAIDminusIAA_asy_rep3; Mus musculus; RNA-Seq
RNA was extracted with 500 μl TRIzol (ThermoFisher, 15596026), according to the manufacturer's protocol, and treated with DNAse I (Qiagen,) for 20min at 37°C. Following phenol:chloroform purification, RNAs were resuspended in Ultrapure DNAse/RNase Free Distilled Water (Thermo, 10977035) Ribo-depleted, stranded and paired-end RNA-seq libraries were prepared and sequenced by Novogene Co Ltd