GSM5138750: Meso_T48_WT_Rep2; Mus musculus; RNA-Seq
Total RNA was extracted by using TRIzol reagent (Invitrogen), according to manufacturer's protocol. Quantity and quality of the starting RNA were checked by Qubit and Bioanalyzer (Agilent). 1 μg of total RNA was subjected to poly(A) selection, and libraries were prepared using the TruSeq RNA Sample Prep Kit (Illumina) following the manufacturer's instructions. Libraries were sequenced on Illumina NextSeq 500 System (single-end 75 bp reads).