GSM5493403: tgt_LB rep3; Escherichia coli; RNA-Seq
RNA was stabilized with RNAprotect Bacteria Reagent (Qiagen #76506) and isolated using the RNeasy Mini Kit (Qiagen # 74104). The quality and quantity of total RNA was evaluated using the Agilent RNA 6000 Nano Kit on an Agilent Bioanalyzer. rRNA was removed using Ribo-Zero™ Magnetic Kit* (Gram-Negative Bacteria) Epicentre. The RNA sequencing library was prepared with TruSeq RNA Library Prep Kit v2 (Illumina). Libraries were analyzed on a Bioanalzyer for average base pair distribution, and quantified using Qubit Fluorometric Quantitation (Thermo Fisher Scientific). TruSeq RNA Library Prep Kit v2 (Illumina): Oligo-dT beads capture polyA tails. Captures the coding transcriptome (without strand information).