GSM6257323: Spleen mature basophil_03; Mus musculus; RNA-Seq
Basophil progenitors, immature basophils and mature basophils were isolated from the bone marrow and mature basophils were isolated from the spleen by FACS sorting. Library construction for bulk-RNA sequencing was conducted according to the previous study (Ogawa et al. Biochem Biophys Res Commun. 2022). Briefly, 100ng of amplified cDNA libraries were subjected to Illumina library construction by using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs). Fragmentation, end-repair, dA-tailing, adaptor ligation, and barcoding PCR were sequentially conducted according to the manufacturer's instruction.