GSM6043327: mTSC, CUT&Tag, IgG, rep 1; Mus musculus; OTHER
Cells were bound to BioMagPlus Concanavalin A coated magnetic beads and incubated overnight with primary antibodies. Samples were washed and incubated with secondary antibody (Guinea Pig anti-Rabbit IgG ABIN101961) for 1 hr, then washed and incubated with pA-Tn5 loaded with Illumina NEXTERA adapters, followed by tagmentation. Adapter-ligated DNA was released with proteinase K and phenol/chloroform extracted. NEXTERA-tagmented libraries were amplified using NEBNext HiFi 2x PCR Master mix.