GSM5020489: OPH3; Mus musculus; RNA-Seq
After 6 days of caspase inhibition, the cultured cells were harvested into 350 µl RLT lysis buffer (Qiagen) with β-mercaptoethanol (Sigma-Aldrich). RNeasy Mini Kit (Qiagen) with included DNase treatment was used for RNA isolation. The library for sequencing was constructed using QuantSeq 3´mRNA-Seq Library Prep kit FWD for Illumina (Lexogen) and Lexogen i5 6 nt Unique Dual Indexing Add-on kit (Lexogen).