GSM5866797: LPS_m6A_IP; Mus musculus; OTHER
Total RNA were extraction follows TRI reagent protocol (Thermo-Fisher) and then treated with DNase I to remove DNA contamination. . For m6A MeRIP-seq, the m6A enrichment were performed using m6A antibody (m6A antibody cat#202203, synaptic systems) and m6A-enriched and fragmented RNA was purified using RNeasy Mini Kit as IP. KAPA Stranded RNA-seq Kit (Illumina) was used for the library preparation of both m6A-IP RNA and input mRNAs. The quality of the completed libraries was performed with Agilent 2100 Bioanalyzer