GSM5019865: differentiation DM3; Bubalus bubalis; RNA-Seq
Cells were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent(Invitrogen，Carlsbad，CA，USA). 3 μg RNA per sample was used as the initial material for RNA sample preparation. PolyA-Seq libraries were prepared following the described protocol at RiboBio (Guangzhou, China) in accordance to the manufacturer's instructions. The identification of mRNAs and lncRNAs were carried out with reference to RiboBio's technical methods. RNA libraries were prepared for sequencing using standard Illumina protocols