GSM4910054: Microglia hC4A/- 2; Mus musculus; RNA-Seq
Microglia were first sorted from frontal cortex homogenates using the FACSAria. For each mouse, 1,000 microglia were sorted based on CD11bhigh and CD45low expression into 20 μl guanidine thiocyanate buffer (Qiagen) supplemented with 1% β-mercaptoethanol. RNA was isolated using RNAClean XP beads (Agencourt A63987). Template switching, cDNA synthesis, cDNA amplification and libraries were prepared using the Nextera kit (Illumina), following the manufacturer's instructions.