GSM6042893: Mouse # 66624 CAF ATAC-seq; Mus musculus; ATAC-seq
5 × 10^4 sorted CAFs were processed for ATAC-seq as described in PMID: 26083756: Buenrostro JD, Wu B, Litzenburger UM, Ruff D, Gonzales ML, Snyder MP, Chang HY, Greenleaf WJ. Single-cell chromatin accessibility reveals principles of regulatory variation. Nature. 2015 Jul 23;523(7561):486-90. doi:10.1038/nature14590. Epub 2015 Jun 17. Libraries were constructed as described in PMID: 26083756: Buenrostro JD, Wu B, Litzenburger UM, Ruff D, Gonzales ML, Snyder MP, Chang HY, Greenleaf WJ. Single-cell chromatin accessibility reveals principles of regulatory variation. Nature. 2015 Jul 23;523(7561):486-90. doi:10.1038/nature14590. Epub 2015 Jun 17. Libraries were subjected to double-sided size selection using SPRIselect beads (Beckman Coulter) at ratios of 0.55 and 1.2X prior to sequencing.