GSM5710168: Control rep1; Danio rerio; RNA-Seq
Embryonic hearts were lysed in TRIzol reagent (Invitrogen) and total RNA purified by RNA microPrep (Zymo Research, R2062) for each biological replicate. Purified RNA eluates were kept at -80C prior to sequencing. RNA samples were analyzed by TapeStation (Agilent) and libraries were generated and sequenced using Ultralow Input RNA-seq (Genewiz). RNA was processed with poly(A) selection for full length transcripts. Libraries were sequenced on an Illumina HiSeq platform using the 2x150bp configuration with single index.