GSM5492222: Primary_RA_Day3_H3K27ac_ChIPseq; Mus musculus; ChIP-Seq
PLZF/RARA transformed progenitors (late promyelocytes: Cd45.2+, C-Kit+, Gr1+, Cd34-, FcγR+) were purified using the FACS Aria III cell sorter (Beckman Dickinson). Cell lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Genomic DNA was purified using the i-Pure kit (Diagenode) Libraries were generated using the MicroPlex Library Preparation Kit (Diagenode) following the manufacturer's instructions and analyzed on a 2100 Bioanalyzer system (Agilent) prior sequencing.