GSM5903326: Control; Mus musculus; ChIP-Seq
Start with two confluent 150 cm2 dishes (1x107- 5x107 cells per dish).  Cross-link proteins to DNA by adding formaldehyde drop-wise directly to the culture media for a final concentration of 1% and rotate gently at room temperature (RT) for 10 min. Add glycine to a final concentration of 125 mM to the media and incubate with rotating for 5 min at RT. Libraries were  constructed according to TacGen