GSM4975187: RNA-seq LGM exp rep 2; Homo sapiens; RNA-Seq
600,000 cells from human myoblast cell lines CMD, LGMD2i and control were harvested and RNA extracted using RNAeasy Micro kit (QIAGEN, Cat 74004). RNA concentrations were measured using Quibit RNA HS Assay Kit (Life Technologies, Cat Q32855) on a Denovix Fluorometer. Samples were processed with 200ng of total RNA. PolyA RNA seq libraries were generated using IIlumina TruSeq Stranded mRNA Sample prep, protocol 15031047 RevE Oct 2013. Libraries were prepared according to the Illumina standard RNA protocol. Each library was single-end with a 76 nt read length.