GSM4845302: WT_heat_2nd; Arabidopsis thaliana; RNA-Seq
Total RNA was prepared from seeds using Trizol (TaKaRa, Tyoko, Japan). mRNA collectd from seeds was fragmented and used as a template for synthesis of cDNA using random hexamers and reverse transcriptase. Second-strand cDNA was synthesized using DNA polymerase I (New England BioLabs, Ipswich, MA, USA) amd RNase H (Invitrogen, Carlsbad, CA, USA). The resultant cDNA fragments were purifed, end-reparided, polyA-tailed, and ligated to index adapters following the Illumina protocol.