Illumina HiSeq 2000 paired end sequencing; Transcriptome of AM/NM roots exposed to high Pi in Lotus japonicus
Four-week-grown AM/NM roots 0, 3, 6, 12, 24 h and 7 d after high Pi application Total RNA was isolated using RNAiso Plus (Takara) and Fruit-mate (Takara). Genomic DNA was removed from the RNA solution by digesting with RNase-free DNase (Qiagen) on an RNeasy column (Qiagen), and the RNA was then purified by the column. The purified RNA was checked for the integrity by capillary gel electrophoresis (Bioanalyzer, Agilent Technologies). cDNA libraries for RNA-seq were constructed using a TruSeq RNA Sample Prep Kit (Illumina).