GSM5444227: miR-7-5p_rep2; Homo sapiens; RNA-Seq
For mRNA-seq, total RNA was extracted with Trizol reagent (Qiagen) according to the manufacturer's instructions. After total RNA extraction, mRNA was selected using Dynabead (Thermofisher). Isolated mRNA was then randomly fragmented using fragmentation reagent (Invitrogen). Size-selected RNA fragments (200-400nt) were used for library preparation. poly(A)-selected RNA-seq Total RNA was extracted using TRIzol (Invitrogen). Both of the library preparations (mRNA-Seq and sRNA-Seq) were done based on the Illumina Truseq small RNA library preparation protocol (TruSeq Small RNA Library Prep Guide, Part # 15004197 Rev. G). DMS-seq libraries were prepared using adaptors from a previous study (PMID: 30605524) following procedures from Rouskin et al., 2014 (PMID: 24336214). mRNA-seq, sRNA-seq, DMS-seq