GSM6042806: IgG_rep1; Homo sapiens; ChIP-Seq
Lysates were clarified from sonicated nuclei and GFI1-Flag-DNA complexes were isolated with anti-Flag affinity gel. Libraries were prepared according to Illumina's instructions accompanying the Paired-End DNA Samples Prep Kit. Briefly, DNA fragment end repair, add A base to 3'end, and connect sequencing adapter. PCR amplified with Illumina primers for 15 cycles and library fragments of ~250 bp were band isolated from an agarose gel. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Illumina HiSeq 2500 following the manufacturer's protocols.