GSM5709973: Pancreas tumor: Hot_1 [S1]; Mus musculus; RNA-Seq
FFPE sections on Superfrost Plus microscope slides were collected via mechanical scrapping, and RNA was isolated using a RNeasy FFPE kit (Qiagen, catalog 73504) following the manufacturer's protocol. Sequencing libraries were prepared using a QuantSeq 3' mRNA-Seq library prep kit FWD for Illumina (Lexogen) following manufacturer's protocol and analyzed on a NextSeq 500 sequencing system (Illumina).