GSM5020495: T1; Mus musculus; ncRNA-Seq
Total RNA in the BV2 cells was isolated using TRIzol reagent. The quality and quantity of the RNA was assessed using a NanoDrop ND-1000 spectrophotometer. Total RNA was removed using the Ribo-Zero Magnetic Gold Kit. The processed product RNA was constructed with KAPA Stranded RNA-Seq Library Prep Kit. The constructed library was tested by Agilent 2100 Bioanalyzer and the library was quantified using quantitative PCR.