GSM4974121: RM01_CP_DNase-seq; Macaca mulatta; DNase-Hypersensitivity
Cells were fixed by final concentration of 1% formaldehyde (Sigma-Aldrich, F1635), mixed for 10 mins at room temperature. Fixation was quenched using 2.5 M glycine at room temperature for 5 mins and immediately centrifuged at 800g for 10 mins. Supernatant was removed and frozen in liquid nitrogen and stored at −80°C until further use. One million fixed cells were lysis, add 25U DNaseI, incubate at 37℃ for 5mins. Extract the DNA, end repair, add A, add adaptor reaction and PCR amplification. Purify size selected DNA using Qiagen Gel Purification Kit. The libraries were sequenced by Illumina PE150 bp paired-end sequencing platform.