GSM5710524: kidney cortex-control diet (4414-S1); Mus musculus; RNA-Seq
MD cells (green)  were isolated by FACS. RNA sequencing was prepared using 10x Genomics 3' v3.1 (cat# 1000092) following manufacturer's protocol.  MD-GFP mice on C57BL6/J background were generated by intercrossing nNOS/CreERT2 and mTmG/fl mice resulting in membrane-targeted eGFP expression in MD cells (green), and membrane-targeted tdTomato expression in all other kidney cell types (red). Samples were parsed into single cells using 10x Genomics Chromium Controller and libraries were simultaneously prepared. Prepared single cell RNA sequencing libraries were sequenced on the Illumina Novaseq 6000 platform at a read length of 28x90 and read depth of 100,000 reads/cell for 2000-4000 cells. single cell RNAseq