GSM6185865: M7-IOL-5dpi; Mus musculus; RNA-Seq
Hearts were collected in 500 μl of TRIzolTM (Invitrogen) with one 5 mm stainless steel bead and processed as described above. RNA was extracted using Zymo direct-zol RNA purification kit (Zymo research, #R2072) according to the manufacturer's instructions A total of 500 ng per sample was sent to the NYU Genome Technology Center for quality control assessment and automated TruSeq stranded total RNA with RiboZero Gold library preparation. Illumina Novaseq600 sequencing (single pair 100)