GSM5903079: H1299 cells, siCLOCK, rep2; Homo sapiens; RNA-Seq
Total RNA was isolated using Trizol reagent (Invitrogen). Double stranded PCR products were heated denatured and circularized by the splint oligo sequence to get the library. The single strand circle DNA (ssCir DNA) was formatted as the final library. The library was amplified with phi29 to make DNA nanoball (DNB) which had more than 300 copies of one molecular, DNBs were loaded into the patterned nanoarray and pair end 150 bases reads were generated on BGIseq500 platform.