GSM4910674: RNAseq.ctrl.unf_egg.rep1; Drosophila melanogaster; RNA-Seq
RNA extraction was performed as in Zenk et al., 2017. Briefly, chloroform extraction was performed followed RNA precipitation with Isopropanol. Samples were then washed twice with 80% EtOH and resuspended in TURBO DNase (Ambion AM2238) reaction mix. Finally, DNase was inactivated. RNA libraries were prepared using the Illumina TruSeq Stranded Total RNA protocol