GSM5325925: P24 knockout islets rep1; Mus musculus; RNA-Seq
Islets from 3 control and 3 Dpy30 knockout male mice were isolated at P24 and 100-200 islets per mouse were handpicked into 1 mL of TRIzol reagent (Thermo Fisher Scientific). Extracted RNA was treated with TURBO DNase I (Thermo Fisher Scientific) for 20 minutes at 37°C to remove genomic DNA. For each sample at P24, mRNA was enriched from 1000 ng of total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) and 6 cDNA libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) with 9 amplification cycles.