GSM4974113: RM02_GZ_Hi-C; Macaca mulatta; Hi-C
Cells were fixed by final concentration of 1% formaldehyde (Sigma-Aldrich, F1635), mixed for 10 mins at room temperature. Fixation was quenched using 2.5 M glycine at room temperature for 5 mins and immediately centrifuged at 800g for 10 mins. Supernatant was removed and frozen in liquid nitrogen and stored at −80°C until further use. Fixed cells, lysis and digest the chromatin. Next, fill-in Biotin and religated in by T4 DNA ligase. Extract the DNA, shearing the DNA to 200-300 bp, size selection. Biotin pulldown. End repair, add A, add adaptor reaction and PCR amplification, DNA products size selection and sequencing.