GSM4844494: ATCC-DYS0100 hiPSCs, transduction 2, TF induction 5, TRA-1-60 low; Homo sapiens; OTHER
Genomic DNA was extracted using QIAGEN DNeasy Blood & Tissue Kit. TFs were amplified by quantitative PCR using universal primers. 1 µg of PCR product per sample was sheared to an average of 200bp on a Covaris sonicator E220 and used for library preparation using NEBNext Ultra DNA Library Prep. Libraries were sequenced on the Illumina NextSeq on High Output Mode following the manufacturer's protocols. DNA-seq