GSM6043309: hTSC, CUT&RUN, TFAP2C; Homo sapiens; OTHER
Cells were bound to BioMagPlus Concanavalin A coated magnetic beads and incubated overnight with primary antibodies, then washed and incubated with pA-Mnase followed by digestion (100mM CaCl2) and reaction arrest in digitonin/EGTA STOP buffer. DNA was extracted using phenol/chloroform. Libraries were constructed with the NEBNext Ultra II DNA Library Prep Kit.