GSM4175981: ON3_Final; Mus musculus; RNA-Seq
Single cells were digested from tissues by mechanical digestion of tissue followed by incubation in a digestion buffer made of 1 mg/mL collagenase, 20 μg/mL hyaluronidase, 50 μg/mL DNase Type I, in RPMI + 1% FBS. The cells were then passed through a 40 μm cell strainer. Dead cells were removed using MACS® Dead Cell Removal Kit. The single cells were run on the 10X Genomics Chromium platform, resulting in a barcoded cDNA library.