GSM5866109: macaque cells sampling at embryonic 34 day_1; Macaca mulatta; RNA-Seq
Single-cell RNA-seq library was prepared and performed on the Chromium platform(10X Genomics), using the single cell expression 3' profiling chemistry combined with cell hashing. Cell-hashing antibody (HTO)-labeled cells from 2~3 tissues were pooled together and washed with RPMI-1640 immediately before loading on the 10X controller.Then library was constructed following the manufacturer's protocol, with some additional steps for the amplification of HTO barcodes.