GSM5866406: MCF10A cells, High-resolution phototagging rep 2; Homo sapiens; RNA-Seq
Cells were harvested using trypsin-EDTA without phenol red (Sigma), centrifuged and resuspended in HBSS buffer (Gibco). Live single cells (validated by Draq7 viability staining) were  sorted into 384-well plates using the BD FACSMelody Cell Sorter (BD Biosciences), spun-down and stored at −80°C. Library preparation and single-cell RNA sequencing was performed by Single Cell Discoveries (Utrecht, Netherlands) using their custom SORT-seq protocol. cDNA libraries were sequenced at 150 k reads/cell on the Illumina NextSeq 500 platform.