GSM6186173: P.aeruginosa-infected lungs, Day 4, biological rep 1; Mus musculus; RNA-Seq
Total RNA was extracted from frozen mouse lung tissues using TRIzol (Thermo Fisher). The integrity of the total RNA and level of DNA contamination was assessed using an Agilent 2100 bioanalyzer (Agilent Technologies) and Qubit 2.0 fluorometer (Invitrogen). Library preparation was performed according to the mRNA-seq sample preparation kit (Illumina).