GSM5059810: 6056_21: Primary MEFS DM-1 C; Mus musculus; RNA-Seq
MEFs were trypsinised and collected in medium, washed once in PBS and lysed in RLT buffer supplemented with 10% 2-Mercaptoethanol. RNA was stored at -80C. Strand-specific cDNA libraries were generated using the TruSeq Stranded mRNA sample preparation kit (Illumina) according to the manufacturer's protocol.