GSM5060273: scATAC-B; Homo sapiens; ATAC-seq
snATAC-seq libraries were generated from frozen human cortical brain tissue using six different experimental conditions: 1spin/FANS/DAPI: 1x ultracentrifugation spin followed by FANS sorting with DAPI; 1spin/FANS/7aad: 1x ultracentrifugation spin followed by FANS sorting with 7aad; 1spin/FANS/scatter: 1x ultracentrifugation spin followed by FANS sorting by scatter (i.e. in the absence of a fluorescent dye); 2spins/no FANS: 2x ultracentrifugation spins without FANS; 2spins/FANS/DAPI: 2x ultracentrifugation spins followed by FANS sorting with DAPI; and 2spins/FANS/scatter: 2x ultracentrifugation spin followed by FANS sorting by scatter Nuclei were hash tagged and partitioned using 10x Genomics B chips. Libraries were generated according to 10x Genomics recommendations via Chromium Single Cell 3ʹ GEM, Library & Gel Bead Kit v3 (PN-1000075)