GSM5768780: MCAO-2; Rattus norvegicus; miRNA-Seq
Total RNA was isolated from  tissues using the Magzol Reagent (Magen, China)according to the manufacturer's protocol. Briefly, total RNAs were ligated with 3' RNA adapter, and followed by 5' adapter ligation. Subsequently, the adapter-ligated RNAs were subjected to RT-PCR and amplified with a low-cycle. Then the PCR products were size selected by PAGE gel according to instructions of NEBNext Multiplex Small RNA Library Prep Set for Illumina (Illumina, USA)