Fresh prostatic tissues from 12-week-old mice were dissociated to form a single cell suspension. Cell preparation for 3' barcoded scRNA-seq (#120237, Chromium V2 assay) was performed according to the manufacturer's protocol (10X Genomics) targeting 5000 cells from single-cell suspensions of freshly processed prostate lobes. Library construction for 3' barcoded scRNA-seq (#120237, Chromium V2 assay) was performed according to the manufacturer's protocol (10X Genomics).