Library preparation was performed using the 10X Chromium VDJ + 5' Gene expression v.1 platform, up to 17,500 cells were partitioned into nanoliter Gel-bead-in-Emulsions (GEMs) droplets. Each GEM undergoes retro-transcriptase reaction to generate single-cell cDNA and receives a unique 12 nt cell barcode and unique molecular identifier (UMI). GEM cDNA was amplified for 11 cycles before being purified using SPRIselect beads. 10μl of purified cDNA was used to 5 generate the library for RNA sequencing. Libraries were sequenced on a NovaSeq S4 (Illumina, 300 cycle kit) flow cell, targeting 50K read pairs/cell.