Dissected ectoplacental cones of E7.5 embryos either from Eed control (Eedfl/fl) and ∆Eed (Eeddl/dl) were used for total RNA extraction using All-prep DNA/RNA micro kit (Qiagen). RNA-seq libraries were prepared with the SMARTer Stranded Total RNA-seq Kit v2 using 10 ng total RNA and sequenced by the NovaSeq 6000 as 100 bp paired-end.