GSM6338514
Total RNA was extracted from HEK293 using TRIzol Reagent (Invitrogen) and genomic DNA was removed using DNase I (Takara). Quality of samples were evaluated using an Agilent 2100 Bioanalyzer (Agilent Technologies) and qualified using the ND-2000(Nanodrop Technologies). An amount of 3μg total RNA was used for RNA-seq transcriptome library preparation following TruSeq RNA sample preparation Kit from Illumina (San Diego, CA).After quantified by TBS380 (Picogreen), paired-end RNA-seq sequencing library was sequenced with the Illumina NovaSeq 6000 platform sequencer (2 ×150bp read length). The raw paired end reads were trimmed and quality controlled by Cutadapt (v1.15) software to get high quality sequence (Clean Data) for further analysis .