p53Q/-_Nutlin_rep3_p
mouse housing: IVC barrier housing criteria for the dissection decision: randomly selected mice with a defined tumor burden Mice were sacrificed and colorectal tumors harvested 4 hrs after the last Nutlin-3a treatment. AOM/DSS treatment to induce colorectal tumors in mice.  na TRIZOL reagent from Invitrogen Library construction performed by Novogene NEB Next® Ultra™ RNA Library Prep Kit  Library quality control: Agilent bioanalyzer 2100, DNA high-sensitivity chip  Concentration checked by qPCR.