GSM6215370
Total RNA-seq libraries were prepared as previously described (Brind'Amour et al., 2018). Briedly, RNA was extracted from mouse embryos or cells using TRIzol (Thermo, 10296028). Ribosomal RNA was then depleted using the NEB Next rRNA Depletion Kit (NEB, E6310S). NEB Next first-strand (NEB, E7771S) and second-strand synthesis modules (NEB, E7550S) were used to synthesize cDNA. Library preparation was performed using NEBNext Ultra II DNA Library Prep Kit (NEB, E7645S) according to the manufacturer's instructions. All libraries were sequenced by Illumina Hi-Seq Xten or Novaseq 6000 according to the manufacturer's instructions.