T-LGL and healthy control CD8 TEMRA cells were lysed in Trizol (Invitrogen) and total RNA was isolated according to manufacturer's protocol. Quality and concentration of total RNA of the samples was determined with the RNA 6000 Nano assay on an Aligent 2100 Bioanalyzer (Agilent Technologies). cDNA libraries were generated with 1 ng total RNA using the Smart-Seq V4 Ultra low input RNA kit (Takara-bio) in combination with the Truseq nano DNA library prep kit (Illumina).