GSM5769315
THP1 cells were seeded into 6 well and induced to be attacted. then, the cells were transfected with si-negetive control (si-NC) or si-MED23 siRNA. After 48 hours, the cells were washed by cold 1X PBS buffer. the RNA was extracted using Trizol (Thermo Fisher Scientific，15596018). The sequencing library was conducted by NEBNext UltraTM RNA Library Prep Kit (NEB #E749), and purified by AMPure XP system, according to the standard Illumina protocols.