Three days post transfection, total RNA was harvested using TriFast reagent (manufacturer's instructions were followed) mRNA libraries were generated from 100ng total RNA input using Quant Seq 3'mRNA Library Prep Kit FWD for Illumina (Lexogen, Austria) according to the manufactures declaration. Libraries were amplified with 16 cycles using barcoded Illumina reverse primer (i7 index primer) in combination with Illumina forward primer.