Brains were collected and divided into two halves. Left hemispheres were immediately homogenized in 1 ml QIAzol (QIAGEN) and RNA was isolated using the RNeasy RNA isolation kit (Quiagen GmbH). Library preparation was performed using QuantSeq 3' mRNA-Seq Library Prep Kit (Lexogen) with an input of 100 ng total RNA according to manufacturer's instructions.