GSM6191347
Total RNA was extracted using 750 µl of TriReagent solution (Thermo Fisher Scientific, Waltham, MA, USA), precipitated with 2-propanol, and washed with 70% ethanol. The air-dried precipitate was suspended in nuclease-free water. Total RNA for sequencing was subjected to DNA digestion using RNase-free DNase I (Thermo Fisher Scientific) according to the manufacturer's instructions. RNA concentration and purity (the 260 nm/230 nm and 260 nm/280 nm values) of each sample were estimated in NanoDrop ND‐2000 spectrophotometer (Thermo Fisher Scientific), while the RNA quality was assessed with 0.7% gel electrophoresis. One hundred ng of total RNA was subjected to the preparation of the library using TruSeq Stranded Total RNA with Ribo-Zero Plant (Illumina, San Diego, CA, USA).