GSM5769928
Fresh cells (2x105 to 5x105 per treatment) were harvested and washed twice in 1.5 Ml wash buffer (20mM HEPES pH 7.5; 150mM NaCl; 0.5mM Spermidine (Sigma S2501); 1× Protease inhibitor cocktail, Roche), and Cut&Tag libraries generated, following the protocol “Bench top CUT&Tag V.2” (Hatice S Kaya-Okur, Steven Henikoff 2019. Bench top CUT&Tag. protocols.io, dx.doi.org/10.17504/protocols.io.bcuhiwt6) Cut&Tag libraries generated, following the protocol “Bench top CUT&Tag V.2” (Hatice S Kaya-Okur, Steven Henikoff 2019. Bench top CUT&Tag. protocols.io, dx.doi.org/10.17504/protocols.io.bcuhiwt6)