WT2_s
centrifugation of exponential-phase cultures in EMJH medium After treatment of cells with Trizol, ucleic Acids were extracted with chloroform and precipitated with isopropanol The QIAseq Fast Select -5S/16S/23S (QIAGEN) kit was used for ribosomal RNA depletion according to manufacturer instructions. Libraries were built using a TruSeq Stranded mRNA library Preparation Kit (Illumina, USA) following  the Qiaseq Fastselect -5S/16S/23S protocol recommendations. Quality control was performed on a Fragment Analyzer