RNAs were purified from the ex vivo isolated IL17eGFP+ wild type (WT) or Rora knock-out Th17 cells from the draining lymph nodes or spinal cords of immunized 1:1 (WT:KO) mixed bone marrow  chimeras at peak of Experimental autoimmune encephalomyelitis (EAE) RNA-Seq libraries were prepared with the SMART-Seq® v4 PLUS Kit (Takara, R400752) following the manufacturer's protocols.