Tissue specimens were formalin fixed and embedded in paraffin. RNA was extracted from the FFPE samples using RecoverAllâ¢ Total Nucleic Acid Isolation  Kit. KAPA  RNA Hyper with RiboErase (KAPA Biosystem) Kit was used for further depletion of ribosomal  RNA and library preparation. Different adaptors were used for multiplexing samples in one  sequencing run. Illumina Library prep kit