GSM6215493
Total RNA is extracted from P1 lung fibroblasts attained from mice with EphB4 Col1a2-driven deletion or control, and then bleomycin treatment or control by standard Trizol method and quantified using the Qubit RNA BR assay (Denovix). Quality of RNA is assessed using the Agilent RNA Nano chips on a Bioanalyzer instrument. For all the samples, RNA integrity number (RIN) was >9. Total RNA libraries are prepared from 200 ηg total RNA using the TruSeq Stranded total RNA library preparation kit (Illumina Cat# 20020596) as per manufacturer's recommendations.  Individual libraries are quantified using a fluorometric high-sensitivity dsDNA assay (Denovix). Library quality is assessed on a high sensitivity DNA chip on the Agilent 2100 Bioanalyzer system (Agilent). For sequencing, samples from each group are divided into two groups of 8 and 9 samples each. Libraries prepared for sequencing (volumetrically pooled, diluted and denatured): 75 paired end read-cycles on an Illumina NextSeq 550 sequencer.