GSM5905892
Macrophages were lysed in two steps using the lysis buffer with 1% NP-40, low speed centrifugation, and addition of 1,2-diheptanoyl-sn-glycero-phosphocholine (30 mM). After preclearing by high-speed centrifugation, the lysates were divided for total RNA isolation and  affinity purification of translated RNA using EGFP-tagged ribosomal protein L10a. Both RNA fractions were isolated using Direct-Zol RNA MiniPrep Plus (Zymo Research). Poly(A) enriched cDNA libraries were prepared according to low input Illumina's protocol.