RNA isolated using RNAzol Ribosomal RNA was depleted from HCT116 wild-type and KO-CREB1 cells by using a Ribo-Zero rRNA Removal Kit (Epicentre). Then, the RNA was used as input for the Illumina TruSeq® Stranded Total RNA HT/LT Sample Prep Kit (Illumina). Libraries were pooled and sequenced on an Illumina Hiseq 3000 PE150 platform.