Total RNA was extracted from 2-week-old Scx KO and wild type littermates (n = 2) using TRIzol reagent (Invitrogen 15596026) mRNA is isolated by NEBNext Poly(A) mRNA Magnetic Isolation Module (New England BioLabs, E7490) and DNA libraries were prepared using NEBNext Ultra Directional RNA Library Prep Kit (New England BioLabs, 7760)