GSM5906035
Cell were disassociated with a 1.2 U/mL Dispase II, 1mgl/mL Collangenase IV, and 5U/mL DNAaseI cocktail. Cell suspension was cryopreserved in liquid nitrogen. For library construccion cells were thawed at 37C, washed and resuspended in PBS+0.04% BSA. 10x Genomics Single cell RNAseq