GSM6338462
To extract the RNA, 500 µl TRIzol (Thermo Scientific) was added and the cells were disrupted within the TRIzol using a mill mixer at 30 Hz for 5 minutes at room temperature. RNA was further purified using chloroform and the RNeasy Micro Kit (Qiagen). cDNA synthesis and amplification was performed using the NEBNext® Poly(A) mRNA Magnetic Isolation Module followed by the NEBNext® Ultra™ II Directional RNA Library Prep and NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1) paired-end, 150 bp