GSM5769357
Isolation of mRNA was then performed using TRIzol® (Invitrogen) according to the manufacturers' procedures. RNA sequencing was performed by next generation sequencing (NGS) using the Illumina NextSeq technology at the Ramaciotti Centre for Genomics. Sequences were trimmed using Trim Galore (version 0.4.4) and genome-guided alignment to a human reference (HG38) was performed using the STAR software (version 2.5). Volume: 20 ul, Concentration:  100 ng/ul