RNA extraction from PBMCs was performed using Trizol reagent (Invitrogen™) according to manufacturer instructions. RNA concentration was evaluated using a NanoDrop DS-11 Spectrophotometer (DeNovix), whereas RNA quality was checked on agarose gel 1%. For sequencing, 500ng of fresh RNA input extracted from PBMCs have been used. For library preparation, KAPA mRNA HyperPrep Kit for ILLUMINA has been used. Sequencing has been performed using NextSeq 500 ILLUMINA platform. All samples passed quality control analysis.