GSM6043603
RNA was extracted using the RNEasy Plus Mini Kit (Qiagen), flash frozen, and submitted to GeneWiz for sequencing. 2 μg of total RNA was used for library construction by GeneWiz. rRNA was removed by PolyA selection for eukaryotic mRNA. cDNA syntehsis, end repair, 5' phosphorylation, dA-tailing, adaptor ligation, and PCR enrichment were carried out by GeneWiz.