Trizol reagent (Sigma) was used to isolate the total RNA of samples collected from three controls (C1, C2 and C3) , three HS0.5 groups (HS0_1, HS0_2 and HS0_3) and three HS0.5-R36(HS36_1, HS36_2, HS36_3) groups of iSCs. RNA libraries were prepared for sequencing using standard Illumina protocols