GSM5905931
Total RNA was extracted from 50-100 mg seedlings using the Zymo Research Quick-RNA Miniprep Kit with on-column DNase I digestion. RNA quality was assessed on the Agilent 2100 Bioanalyzer. Sequencing libraries were generated with 300 ng to 1 µg of RNA using the Illumina TruSeq Stranded mRNA Sample Preparation Kit. The libraries were assessed for size and purity using the Agilent 2100 Bioanalyzer, and quantitated by qPCR using a KAPA Biosystems Library Quantitation kit. Libraries were normalized, pooled, and diluted to a loading concentration of 1.8 pM, loaded on an Illumina High-Output Flow Cell, and sequenced on the Illumina NextSeq 500 instrument.