The cell fractions were separated by centrifugation at 10, 000 rpm for 5 min at 4°C. Total RNA was isolated using a E.Z.N.A.® Bacterial RNA Kit (Omega Bio‐tek, USA) as described by the manufacturer for RNA-seq. A prokaryotic chain specific sequencing library was constructed using a NEBNext Ultra Directional RNA Library Prep Kit and then sequenced on an Illumina HiSeq platform. RNA libraries were prepared for sequencing using standard protocols of NEBNext Ultra Directional RNA Library Prep Kit.