RNA was isolated via Qiagen miRNeasy (micro) performed by the NGS Core Facility University Hospital Bonn according to the maufacturers' user guide for the QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina (https://www.lexogen.com/quantseq-3mrna-sequencing/#quantseqdownloads/) 1x 100bp single reads, 15mio reads deep per sample, NovaSeq 6000