Single keratinocytes from mouse epidermis were isolated by flow sorting using appropriate markers. Cells were sorted into individual wells of a 96-well plate containing 2ul lysis buffer (0.8% (volume/volume) Triton X-100 and 2U/ul recombinant RNase inhibitor Libraries were prepared for sequencing using standard Illumina protocols with SmartSeq2 followed by Nextera XT protocol.