GSM5769297
4x10^5 THP-1 cells were collected, washed by cold 1X PBS. And then, the cell pellets were re-suspended in 50ul cold lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630) to generate the nuclei pellets. Next, the nuclei pellets were resuspended in the transposition reaction mix (25 μl TD, 2.5 μl TDE1, 22.5 μl Nuclease Free H2O), incubated at 37°C for 30minutes. the DNA was purified by Qiagen MinElute PCR Purification Kit The assays for transposase-accessible chromatin with high throughput sequencing (ATAC) assays were performed in Jiayin Biotechnology Ltd