cells were sorted and lysed in 1ul of lysis buffer containing (0.3% NP40, 0.12mM dNTPs, 1U RNase Inhibitor, 0.11 uM Reverse Transcribe primer) CEL-Seq2 protocol is used for single cell analysis with modification of SuperScripII enzyme to MaximaH minus(Thermo Scientific, EP0752) and 2ns strand buffers to 2nd Strand Module (NEB,E6111). Pooling unit is 384 cells in the same 384 plate.