WT_shC_PBS_20_p
Breast tumor tissue was collected at 3 or 11 weeks after grafting in the non-compound treated group, or after 2 weeks in the compound treatment group. Tumor samples were fresh frozen The protocol is described in Sun et al., 2019 (PMID: 31288084). Briefly, silencing of Tnc  in mouse cells was done by short hairpin (sh) mediated gene expression knock down (KD). Lentiviral particles with shRNA vectors (Sigma- Aldrich) specific for Tnc  were used. sh1, TRCN0000312137, sequence 5' -CCGGCCCGGAACTGAATATGGGATTCTCGAGAATCCCATATTCAGTTCCGGGTTTTTG-3'; sh2, TRCN0000312138, 5'-CCGGGCATCAACACAACCAGTCTAACTCGAGTTAGACTGGTTGTGTTGATGCTTTTTG-30. Lentiviral particles encoding a non-targeting shRNA vector were used as a control (SHC202V, Sigma-Aldrich). Transduced cells were selected with normal medium supplemented with 10 m g/ml puromycin (ThermoFisher) and the selection pressure was maintained in all in vitro experiments. Whole RNA extraction from tissues Whole RNAseq