GSM6297333
Chromatin extracts were harvested as previously described (Gregersen et al. 2019 Cell) and used for IP of RNAPII (4H8 antibody coupled to ProteinG dynabead) or double FLAG IP (M2 agarose beads followed by 3x FLAG peptide elution for SCAF4 and SCAF8 samples) followed by a RNAPII 4H8 IP, RNA was extracted directed from beads using Zymo Quick-RNA microPrep. NEBNext small RNA library prep protocol followed by gel purification of 140-230bp PCR products