GSM6215353
Total RNA was isolated using miRVana miRNA isolation kit and stored at -80°C in RNase-free H2O until assayed. Quantity and quality of RNA were assessed using Bioanalyzer 2100 and Qubit 3.0 Fluorometer. One microgram of total RNA was used to construct cDNA libraries with the TruSeq Stranded Total RNA with Ribo-Zero Gold Human/Mouse/Rat kit following manufacturer's protocols.