After 3 or 10days in culture, the oesophageal-derived epithelia were harvested and peeled off from the oesophageal stroma or dermis. The epithelial sheet was dissociated into a single cell suspension and FACS sorted to select live singlets. For each sample, 7,000-9,000 cells were submitted for  10X single cell GEX v3 library preparation. RNA libraries were prepared for sequencing using standard Illumina protocols for 10X Single Cell GEX v3