GSM5905573
A total amount of 2.5 ng RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were prepared with TruSeq Small RNA Sample Prep Kit (Illumina, San Diego, USA). Briefly, the 3' adenylated adaptor and reverse transcription synthetic first chain were ligated, then PCR amplification and the size were selected. PAGE gel was used for electrophoresis fragment screening purposes, rubber cutting recycling as the pieces get small RNA libraries. At last, PCR products were purified and the library quality was sequenced with Illumina hiseq2000 / 2500.