For colon tissue: 30 pinch biopsies were tkaen from the rectoSignoid colon of each patient. Tissue was frozen in liquid nitrogen until use. Thawed tissue was treated with type 4 collagenase for 30 minutes and strained through a 100 micron filter. Cells were stained for Viability and CD45 and sorted for live CD45+ on an Astrios EQ For Peripheral blood: Whole blood was collewcted in a Potassium EDTA tube, blood was layered on ficolla and centrifuged at 1200RPM for 20 minutes. Buffy Coat layer was collected and stained for CD45 and viability. CD45+ live cells were collected on an Astrios EQ.