Following treatment, seedlings were frozen in liquid nitrogen and stored in -80°C. RNA was extracted using RNeasy Plant Mini Kit (QIAGEN, Germany) followed by DNase treatment using an AMBION DNA-free™ Kit (Life Technologies, CA, USA) according to manufacturers' instructions. Libraries were prepared using KAPA Stranded RNA-Seq Library Preparation Kit and sequenced with Illumina® NextSeq® 500.