E01787_p
Bone marrow was isolated from mouse femurs and aliquots prepared in 90% v/v foetal bovine serum (FBS) containing 10% v/v dimethyl sulfoxide (DMSO). Vials were stored in liquid nitrogen until further use. Bone marrow cells were cultured in vitro in the presence of 50 ng/mL recombinant macrophage colony-stimulating factor (MCSF) (Peprotech; London, UK) to induce differentiation of monocytes to macrophages Isolation and purification of RNA were performed using the RNeasy mini kit (Qiagen), as per manufacturer instructions. Strand-specific sequencing libraries were prepared with the TruSeq stranded Total RNA kit (Illumina) from 1 Âµg total RNA of each sample