GSM6339391
RNA were isolated using Qiagen Rneasy mini kit (74106) Ribosome-depleted RNAs were used to generate the cDNA libraries using the KAPA Hyper Prep Kit (KR0961) according to the instructions. Briefly, DNA was end-repaired and A-tailing to yield a protruding 3- 'A' base for ligation of Illumina's adapters which have a single 'T' base overhang at the 3' end. Post-liagation clean was used to remove the extra adapters. Then the adapter ligation DNA was PCR amplified with Illumina primers for 8 cycles, follwed with post-ampilcation clean with KAPA pure beads. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Illumina HiSeq 4000 following the manufacturer's protocols.