the total RNA was extracted using RNeasy Lipid Tissue Mini Kit (Qiagen). sample library was prepared in the 96 well plate by using the Illumina TruSeq Stranded mRNA kit, sample library was prepared in the 96 well plate by using the Illumina TruSeq Stranded mRNA kit, and subsequently sent for pair-end sequencing in Illumina HiSeq 2500 High Output V4 (125bp of each, 2 lanes, The National Genomics Infrastructure, Sweden).