GSM6294222
Total RNA of cells was extracted with TRIZOL reagent according to the manufacturer's protocol. RNA libraries were prepared for sequencing using standard Illumina protocols, mRNA with polyA was enriched by Oligo (DT) magnetic beads from total RNA. Then mRNA was randomly interrupted, linked with connectors and reverse transcribed to cDNA for library construction. Finally, double pair-end (PE) sequencing was conducted based on Illumina NovaSeq sequencing platform.