GSM6191321
Total RNA from HeLa cells was isolated using Trizol reaget (Invitrogen, Cat# 15596-018) following the manufacturer's protocl. 75 µg of total RNA was used to isolate poly(A) mRNAs. 500-1000 ng of poly(A) mRNA was used for library preparation RNA libraries for dRNA-Seq were prepared using direct RNA sequencing kit (Oxford Nanopore Technologies, SQK-RNA002)  following manufacturer's protocols.