P. marinus cells were harvested from day 11 lipid replete and lipid deplete media for each biological replicate. Cells were pelleted at 3,900 RPM for 10 minutes at 4 C° using an Eppendorf 5810R Centrifuge. P. marinus total RNA was extracted per manufacturer's protocol using the Qiagen RNeasy mini- kit; RNA quality was verified on a 1% percent agarose gel and visualized using a UV transilluminator. RNA libraries were prepared for sequencing using standard Illumina protocols