p53+/-_DMSO_rep1_p
mouse housing: IVC barrier housing criteria for the dissection decision: randomly selected mice with a defined tumor burden Mice were sacrificed and colorectal tumors harvested 4 hrs after the last Nutlin-3a treatment. AOM/DSS treatment to induce colorectal tumors in mice.  Mice with a defined tumor burden were orally treated with 150 mg/kg  Nutlin-3a (BOC Biosciences) dissolved in 2% KUCEL / 0.2% Tween-80 over 3 consecutive days. na TRIZOL reagent from Invitrogen Library construction performed by Novogene NEB Next® Ultra™ RNA Library Prep Kit  Library quality control: Agilent bioanalyzer 2100, DNA high-sensitivity chip  Concentration checked by qPCR.