mammary basal cells were routinely sorted by flow cytometer ,cells was immediately lysated into 1ul specific smart seq reagent,and RNA was harvested using Trizol reagent.RNA sequencing libraries were generated using the KAPA Stranded RNA-Seq Kit for illumina with multiplexing primers, according to the manufacturer's protocol. Then sequencing was performed on illumina Nova sequencer. RNA libraries were prepared for sequencing using standard Illumina protocols