GSM6043789
Total RNA was isolated from L. major cultures using TriPure, according to the manufacturer's instructions. 5x107 cells were used for each prep. For mRNA-seq, total RNA was isolated from wild-type and PP1-8e KO L. major cultures using TriPure. Six mRNA-seq libraries were constructed (triplicate samples for WT and PP1-8eKO) using Illumina TruSeq Stranded RNA LT Kit following the manufacturer's instructions with limited modifications. The starting quantity of total RNA was adjusted to 1.3 µg, and all volumes were reduced to a third of the described quantity. High throughput sequencing was performed at the Georgia Genomics and Bioinformatics Core (GGBC) on a Novaseq 6000 (Illumina).