Splenocytes were harvested from mice that were treated with anti-PDL1 or isotype treated antibodies and FACS sorted to obtain virus-specific SMARTA CD4 T cells. Sorted anti-PDL1 treated and isotype treated SMARTA cells were then washed 2x with PBS + 0.04% BSA and mixed with 10X Genomics Chromium single cell RNA master mix and loaded onto a 10x chromium chip according to the manufacturer's protocol to obtain single cell cDNA. Library generation was performed following the Chromium Single Cell 3ʹ Reagents Kits v2 User Guide: CG00052 Rev B.