Total RNAs were extracted from stable transfected GC cells with TRIzol (Invitrogen, USA). Fragmented RNAs were  incubated with m6A antibody for immunoprecipitation by a Magna MeRIP RNA-Bindin Protein Immunoprecipitation Kit according to the manufacturer's instructions (Millipore, USA)(#17–10,499,Merck Millipore, MA, USA). RNA libraries were prepared  using NEBNext® Ultra™ RNA Library Prep Kit for Illumina