Total RNA was extracted and purified using QIAshredder and RNeasy Plus Mini kits. After RNA purification,  it was treated with DNAse I to remove DNA contaminants, purified again on Qiagen RNA easy. Sample was anayzed on bioanlyzer, concentration and purity determined before proceeding with RNA -sequencing. Samples were prepared according to library kit manufacturer's protocol, indexed, pooled, and sequenced on an Illumina HiSeq. RiboErase method  was used for sequencing