GSM5997684
Qiagen Rneasy Mini Kit RNA quality was checked using the Bioanalyzer RNA 6000 Nano kit (Agilent). cDNA libraries were prepared from 100 ng of total RNA. After fragmentation for 5 min using Mg ions the RNA was treated with T4 PNK for phosphorylation/dephosphorylation and RppH for decapping followed by NEBNext® Multiplex Small RNA Library Prep. (New England Biolabs). rRNA depletion was not performed. The number of the PCR cycles was determined to 12 by qPCR and the elongation time was set to 30 sec. Libraries were quantified before and after pooling by QubitTM dsDNA HS Assay Kit (3.0 Fluorometer; ThermoFisher) and quality was checked using Bioanalyzer High Sensitivity DNA kit (Agilent).