GSM5905955
Single cell suspensions were prepared by meshing spleens through a 70um cell strainer and after erythrocyte lysis, CD4+ T cells, defined as CD3+CD4+CD8-CD19-CD25-, were sorted from the cell suspension using a FACSAria II cell sorter (BD Biosciences). Sorted cells were lysed in RLT buffer and total RNA was isolated using the RNeasy mini kit with on-column DNase treatment (Qiagen) RNA seq libraries were generated using the KAPA mRNA Hyperprep kit protocol