Genomic DNA was extracted using QiaAmp DNA Mini Kit 1 µg of genomic DNA was sheared to a mean fragment length of about 140 base pairs using focused acoustic energy (Covaris E210). Exome sequencing was performed by exome capture using the IDT xGen capture probe panel with an additional "spike-in" of ~2,500 regions, totaling ~620 kb, of RefGene coding regions that were not included or were poorlycovered by the IDT panel. Captured fragments were sequenced using 101 base pair paired-end sequencing reads in an Illumina NovaSeq 6000 with a S4 flowcell according to Illumina protocols.