Tissue was fixed in 1% formaldehyde for 10 min at room temperature and quenched with 111 μl 1.25M glycine. Tissue was homogenized in nuclei isolation buffer (50mM Tris-HCl pH8, 60mM KCl, 0.5% NP-40), lysed in lysis buffer buffer (50mM Tris-HCl pH8, 10mM EDTA pH8, 0.5mM EGTA pH8, 0.5% SDS) and sonicated on a Pico Biorupter for  three 8x cycles of 30 sec on, 90 sec off at 4°C Chromatin was then diluted in dilution buffer (20mM Tris-HCl pH8, 2mM EDTA pH8, 150mM  NaCl, 1% Triton X100). After incubation with anti-H3K9ac coated magnetic beads, DNA waswashed,  treated with RNAse and proteinase K and cleaned up with g the Zymo ChIP DNA clean and concentrator kit (Zymo #D5201) following the  manufacturer's protocol. Library was prepared using standard UCSD Genomics core protocols to generate single ended  TruSeq adapters.  Library PCR amplification was performed at 15 cycles and agarose gel size  selection performed at the 300-600 bp range.  Samples were analyzed by Agilent Tapestation to  determine concentrations and loaded into the IGM Core's Illumina HiSeq 4000.