GSM6338763
Melanoma tumors were harvested and place in cold 8%FBS-HBSS until processed within two hours of harvest. Tumors were minced into small pieces and digested for 15 minutes at 37 degrees in 1mg/mL Collagenase H and 200ug/mL DNase in RPMI media. Digests were pushed through 100um strainer and RBCs were lysed. Cell viability was measured (all samples were >70%) and cells were resuspended for scRNA-seq subsequent processing. Single cell libraries were captured and barcoded using the 10X Genomics Single Cell Chromium 3' V3 chemistry reagents kit (10x Genomics, 1000121) according to the manufacturer's protocol.