Total RNA was extracted with TRizol (Thermo Fisher Scientific) and treated with TURBO DNase for 30min at 37°C. Ribosomal RNA was removed using NEBNext rRNA Depletion Kit v2 (NEB), we used 1ug of RNA as input in the QuantSeq 3' mRNA-Seq Library Prep Kit REV (Lexogen) to prepare 3' end libraries. 3'mRNA-seq libraries were generated using 1ug of RNA as input in the QuantSeq 3' mRNA-Seq Library Prep Kit REV from Lexogen.