GSM5870294
Cells were plated on 6-well plates and grown to 70 % density. Cells were washed with PBS, and total RNA was isolated using the RNeasy mini kit (Qiagen) according to the manufacturer's protocol. RNA quality was verified by Agilent 2100 Bioanalyzer. Ribosomal RNA was removed from total RNA using Illumina Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat) according to the manufacturer's protocol. Ribo Depleted RNA was fragmented for 15 min. at 94°C. Libraries were constructed using NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's protocol.