GSM5966542
Total RNA was purified with miRNeasy Micro Kit (Qiagen) from FACS-sorted IECs isolated from small intestine. RNA quality was determined on a Bioanalyzer using the RNA 6000 Pico Kit (Agilent). mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library.