GSM6215572
Transfected cells were isolated by FACS and lysed in cell lysis buffer (10mM TrisHCl ph7.5, 10mM NaCl, 3mM MgCl2, 0.1% NP-40, 0.1% Tween20, 0.1% Digitonin Transposition reactionx mix from Nextera Library Prep kit was used (Tagment DNA Enzyme 1 and Tagment DNA Buffer) Nextera primer sets with NEBNext Master Mix was used to generate libraries with Ampure beads used for cleanup