GSM5743505: FVB_rep1; Mus musculus; RNA-Seq
Hearts were rapidly isolated from euthanized, male, eight-month-old Tg-RKIP, Tg-GRK2 and non-transgenic FVB/N mice, and rapidly frozen in liquid nitrogen. Hearts were pulverized under liquid nitrogen, and total RNA was isolated with the RNeasy midi kit (Qiagen). RNA libraries were prepared for transcriptome sequencing by GATC Biotech AG, an Eurofins Genomics Company, using standard Illumina protocols in frame of the INVIEW Transcriptome Explore protocol. For library preparation, mRNA was fragmented, and random hexamer-primed cDNA synthesis was performed. Illumina single read (SR) sequencing (1 x 50 bp) with guaranteed 30 million reads per sample was performed by the Genome Sequencer Illumina HiSeq, in the sequence mode HiSeq 4000.