GSM5967125: Hpb-CM; Mus musculus; RNA-Seq
Single cell suspensions were prepared by manual disturbance of Matrigel domes followed by incubation in TrypLE (Gibco) for 40 minutes at 370C, were loaded on a Next GEM Chip G (PN-1000120) together with Next GEM Single Cell 3ʹ GEM Kit v3.1 (PN-1000121) and single cells were captured on a 10X Genomics Chromium controller with a recovery target of 5000 cells per sample. cDNAs were generated following the 10X Genomics protocol. cDNAs were size selected using SPRIselect beads from Beckman Coulter (B23318), and their quality was checked with a Bioanalyzer High Sensitivity DNA Kit from Agilent (5067-4626). One quarter of the total cDNA was used to generate libraries using Next GEM Single Cell Library Kit (PN-1000121) and barcoded using the Single Index Kit Set A (PN-111213) following the 10X protocol. Libraries were size selected using SPRIselect beads and their quality was checked with the Bioanalyzer High Sensitivity DNA Kit. Libraries size were centered at 440 bp