GSM5570932: mouse_34; Mus musculus; RNA-Seq
21 days old mice were stratified by body weight and divided into the two dietary groups. The galactose group received a pelleted diet containing 16 en% in galactose + 16 en% in glucose, the glucose group was fed with a pelleted diet containing 32 en% in glucose. The two diets were otherwise identical. The mice were fed for 3 weeks with the intervention diet and sacrificed on day PN42. The RNA used for the analysis was obtained from the scraping of the proximal part of the small intestine. Collected tissue was snap frozen in liquid nitrogen and stored at -80°C until further analysis. Total RNA was isolated with RNeasy columns (Qiagen, Venlo, TheNetherlands). RNA integrity was checked on an Agilent 2200 Tapestation according to the manufacturer's instructions (Agilent Technologies Inc., Santa Clara, CA, USA). RNA preparation, library construction and sequencing on DNBSEQ platform was performed at Beijing Genomics Institute.