GSM5494405: B3_PFC; Rattus norvegicus; RNA-Seq
We performed whole-transcriptome sequencing (WTS) using total RNA from two brain areas (the medial prefrontal cortex (mPFC) and the nucleus accumbens (NAc)) of 19 rGT trained rats (risk-averse, n=9; risk-seeking, n=10). Total RNA was extracted using the miRNeasy Mini kit (Qiagen, Hilden, Germany) and quantity, and quality was measured using the Qubit (Thermo Fisher Scientific, Waltham, MA) and TapeStation (Agilent Technologies, Santa Clara, CA), respectively. Total RNA of 36 rat brain tissues were converted into cDNA library using TruSeq RNA Library Preparation Kit v2 (Illumina, San Diego, CA). After polymerase chain reaction (PCR) amplification, the final product was assessed with a TapeStation (Agilent) and subsequently sequenced on the Illumina NovaSeq 6000 platform (Illumina) with 101-bp paired-end reads.