GSM5832802: miRNA-lung-normal-2(Cap2); Homo sapiens; miRNA-Seq
Total RNA was extracted from human lung tissue samples by using TRIzol reagents (Invitrogen, Waltham, Massachusetts, USA) as recommended by the manufacturer. The total RNA quantity was determined with Nanodrop 2000 (Thermo Scientific, Waltham, Massachusetts, USA) and quality was assessed by running 1.5% agarose gel electrophoresis and staining with 4S Red Plus Nucleic Acid Stain (Sango, Shanghai, China). 1μg of total RNA was used to construct the small RNA-seq libraries by TruSeq® Small RNA Library Prep Kit (NEB, San Diego, California, USA) following the manufacturer's protocol. The quality of libraries was assessed with Agilent 2100 Bioanalyzer (Agilent, United States).