GSM5570871: h1;met1+/- (rna-seq); Arabidopsis thaliana; RNA-Seq
We extracted total RNA from rosette leaves of 4-5-week-old plants using Trizol (Invitrogen, cat. No. 15596026) according to manufacturer's manual. To remove DNA from samples, 10 ug of RNA was treated with DNA-free DNA removal kit (Thermo, AM1907). Ribosomal RNA was depleted using the RiboMinus plant kit for RNA-seq (Invitrogen, cat. No. A1083808). 100 ng rRNA-depeleted RNA was used to construct RNA sequencing libraries with  Ovation RNA-seq systems 1-16 for model organism (Arabidopsis) (Nugen, cat. No. 0351).