Hi-C of Isoetes sinensis
Hi-C sequencing libraries were produced using the following method: 1) Cells were fixed with the cell cross-linking agent paraformaldehyde; 2) The cross-linked DNA was treated with restriction enzymes; 3) Biotin was added to mark the ends of oligonucleotides during terminal repair; 4) Processing with nucleic acid ligase to connect adjacent DNA fragments; 5) Protease digests proteins at the junction point, uncross-linking proteins and DNA, extracting genomic DNA and randomly breaking DNA segments with a growth of 350bp through Covaris crusher, and then recycling. According to manufacturer's instructions (Illumina), the library was sequenced on a HiSeq X DNA sequencer to obtain 150 nucleotide sequences at the pair end.