21 h Sample of Metarhizium acridum
Samples of WT and MaNCP1 strains, which grown on the locust hind wings at 28 for 21 h, were collected to extract RNA for RNA-Seq through DNBSEQ sequencing platform. Prior to mapping to the M. acridum genome and annotated genes, raw data was filtered. In order to removed reads which is low quality, joint contamination or included with unknown base with a high content of nitrogen. The clean data was treated with randomness analysis, query coverage analysis, and saturation analysis before normalized to tags per million (TPM) to obtain normalized gene expression levels. Identification of differentially expressed genes was using a Q-value  0.05 and fold change 2. Differentially expressed genes were classified and annotated using gene ontology (GO) analysis.