GSM5532173: HOTTIP-KO-small-RNA-seq_1; Homo sapiens; ncRNA-Seq
Norgen Biotek RNA purification kit was used to isolate RNA as per manufacturer's instructions. cDNA (reverse transcription - RNA to cDNA) was made using High Capacity RNA to cDNA kit (Life Technologies, Grand Island, NY). Small RNA-seq libraries were prepared using BioO from Perkin Elmer and Qiagen kits. Small RNAs were sequenced using a TruSeq Small RNA Sequencing Kit (Illumina, San Diego, CA, USA) according to manufacturer instructions.