GSM5286040: 1065_T1; Homo sapiens; RNA-Seq
The human blood samples collected into PAXgene Blood RNA Tubes were removed from the -80°C freezer and incubated overnight at room temperature before RNA extraction. RNA was harvested using the RNeasy Tissue Mini Kit (Qiagen), according to manufacturer's instructions. RNA was reverse transcribed to complementary DNA (Lexogen QuantSeq 3′ FWD), and sequenced on a HiSeq 4000 instrument (Illumina). RNA libraries were prepared for sequencing using  manufacturer's instructions  (Lexogen QuantSeq 3′ FWD)