GSM6261376: D02.MCF7.Y117L.factor2.4; Homo sapiens; RNA-Seq
RNA from cell lines was isolated using RNAeasy Plus mini kit (Qiagen, Germany) and mRNA was isolated from 80 ng of purified total RNA using oligo-dT beads (New England Biolabs, Inc) NEBNext Ultra™ RNA kit (New England Biolabs, USA) was used for full-length cDNA synthesis and library preparation. Libraries were pooled, denatured and diluted, resulting in a 2.0 pM DNA solution. PhiX control was spiked at 1%. Libraries were sequenced on an Illumina NextSeq 500 instrument (Illumina Inc) using NextSeq 500 High Output reagent kit (Illumina Inc) (1x75 cycles)