Chromatin panel NGS sequencing of FFPE tumor tissue from chordoma
DNA was extracted from one formalin-fixed, paraffin-embedded chordoma tumor tissue by using GeneRead DNA FFPE kit (Qiagen, Germany). DNA sequencing (NGS) was performed by using Ion AmpliSeq targeted amplification technology with custom panel on 25 genes involved in epigenetic processes and AmpliSeq Comprehensive Cancer Panel (Thermo Fisher Scientific, United States) containing cancer-specific primer panel with exon coverage of 409 oncogenes and tumor suppressor genes. The bioinformatic workflow for sequencing data analysis was based on Torrent Suite software (version 5.10.1).