GSM5381292: starved fasn-1 mutant (D3); Caenorhabditis elegans; RNA-Seq
RNA was extracted using 1ml TRIzol Reagent (Invitrogen# 15596026) according to the manufacturer's protocol with the following exception - 100 uL of acid-washed sand (Sigma# 27439) was added to each sample at the beginning of the protocol to aid homogenization. RNA was eluted in nuclease-free water and stored at -80C until further use. Libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs# E7770) starting with 1 ug total RNA per library and 8 cycles of PCR.