Illumina RNA-seq of Ictalurus furcatus D&B strain: adult female liver
The RNA integrity was evaluated with the LabChip GX Touch HT (PerkinElmer, MA, USA). The RNA library preparation was performed using NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, MA, USA). Enriched RNAs were fragmented for 10 minutes at 94 C. The first strand cDNA synthesis, second strand cDNA synthesis, end repair and adaptor ligation according to the manufacturers protocol. The library PCR amplification were performed with 16 cycle PCR. The average size of the RNA libraries was approximately 350 bp (including the adapters). The RNA sequencing libraries were checked using the LabChip GX Touch HT (PerkinElmer, MA, USA) and quantified by using Qubit 3.0 Fluorometer (Invitrogen, CA, USA).