GSM5743595: 40 day S6 OE rep 3; Drosophila melanogaster; RNA-Seq
Fat bodies were dissected in ice-cold PBS, flash frozen on dry ice, and RNA was harvested using RNAqueous™-Micro Total RNA Isolation Kit (Ambion) , with optional DNase treatment performed. Ovation Universal RNA-seq System (Nuegen) was used with 50 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Tecan and Illumina protocols. Library preparations with Illumina TruSeq RNA Library Prep Kit were performed by GENEWIZ.