GSM5144817: BAN126; Caenorhabditis elegans; ChIP-Seq
Worms were collected in M9, snap frozen and stored at -80°C. Chromatin extracted as previously described (Askjaer, P., et. al. Modern techniques for the analysis of chrmatin and nuclear organization in C. elegans (April 2, 2014), WormBook, et. The C. elegans Research Community, Woembook, doi/10.1895/wormbook.1.169.1, http://wormbook.org The 75-nt sequence reads generated by Illumina sequencing (using NextSeq 500) are mapped to the genome using the BWA algorithm with default settings. Alignment information for each read is stored in the BAM format. Only reads that pass Illumina's purity filter, align with no more than 2 mismatches, and map uniquely to the genome are used in the subsequent analysis. 