NextSeq 550 paired end sequencing; Metagenomic regulatory sequence library characterized in RpoD ortholog strains
Metagenomic regulatory sequence library characterized in RpoD ortholog strains
Samples were harvested during mid-log growth phase DNA samples were extracted using Qiagen plasmid miniprep kit. RNA samples were extracted using the RNA-snap protocol. DNA libraries were prepped through an initial PCR that amplified the regulatory sequence loci of the plasmid library and added a partial illumina adapter sequence. Subsequent PCR added the sample index and the rest of the illumina adapter sequences. RNA libraries were prepped through DNA digestion, reporter gene specific reverse transcription, adapter ligation, initial PCR to amplify reverse transcribed and adapter ligated reporter transcripts and add partial illumina adapter sequences and a subsequent PCR that added sample index sequences and the rest of illumina adapter sequences