GSM5494596: RNA-seq-HBEC-SARS2-4hpi-rep2; Homo sapiens; RNA-Seq
Cytosolic extracts were either subjected to ribo-seq or RNA-seq as in published protocols Infected cells were lysed and two aliquots were processed in parallel for ribo-seq and RNA-seq. For RNA-seq, total RNA was isolated using ZymoClean RNA-Clean and Concentrator kit and subjected to Illumina stranded mRNA prep. Ribo-seq libraries were prepared in house following published protocols. Both libraries were sequenced using Illumina NextSeq following the manufacturer's protocols.