GSM6044032: S16_H3k27ac; Mus musculus; ChIP-Seq
Frozen liver tissues were homogenized in PBS and cross-linked with formaldehyde and the reaction was stopped by the addition of glycine at room temperature. subsequently, liver tissues were lysed and chromatin was extracted and sonicated to generate 200-500 base pair fragments, which were used for immunoprecipitation. DNA libraries were prepared for sequencing using standard Illumina protocols