RNA-seq of G. intestinalis clonal population
To minimize the heterogeneity of expressed VSPs in culture, clonal trophozoite populations were created from the original WB-C6 clone using serial dilution. Total RNA was purified from clonal population by Trizol extraction according to manufacturers protocol. Ribosomal RNA was depleted in all samples and strand-specific Illumina sequencing libraries were constructed. RNA sequencing was performed as a paid service by Genehub.com. The libraries were sequenced as 2x150 bp paired-end reads on an Illumina MiSeq instrument.