GSM5495169: control 180 min; Pseudomonas aeruginosa; RNA-Seq
Total RNA was extracted using Rneasy Mini kit (Qiagen, USA) following protocols provided by the manufacturer. The isolated RNA was stored at -80°C until use. The Ribo-Zero rRNA removal kit (Epicentre, USA) was used for ribosomal RNA depletion according to manufacturer instruction. Libraries for Illumina sequencing were made with the TruSeq Stranded mRNA sample prep kit (Illuminam, USA) following the manufacturer's protocol.