ATAC-seq of Mus musculus: adult hepatocytes
Nuclei were isolated from 25,000 cells for transposition reaction with Tn5 Transposase (Illumina, catalog # FC-121-1030). DNA was purified by using PCR purification kit (Qiagen). Sequencing libraries were generated from purified DNA by amplified with NEBNext high-fidelity 2X PCR Master Mix (New England Biolabs). Sequencing libraries were sequenced using Illumina Nextseq 550 sequencer to obtain 41x41bp paired-end reads.