16S rRNA metagenome of soil bacterial communities from neutral soil of Karnataka, India (1000 mg/kg nZnO)
Microbial DNA was isolated from the soil samples using Nucleospin Soil kit. The qualities of the isolated metagenomic DNA samples were quantified using NanoDrop. The amplicon libraries were prepared using Nextera XT Index Kit (Illumina Inc.) as per the 16S Metagenomic Sequencing Library preparation protocol. Libraries were loaded onto MiSeq (paired end, 2 x 300 bp) at appropriate concentration (10-20pM) for cluster generation and sequencing.