GSM5380065: Fish_Oil_rep5; Mus musculus; RNA-Seq
RNA was extracted from D19 placenta of  CON or Air Pollution particle exposed mice with Dirct Zol RNA MiniPrep kit from Zymo following manufacturer's protocol. Libraries were prepared according to Illumina's instructions accompanying the KAPA Stranded mRNA sequencing kit (# KK8420; Kapa Biosystem, Cape Town, South Africa). Briefly,100ng of total RNA from each sample was used as the starting material with quadruplicate biological replicate from each group. mRNA was captured using magnetic oligo-dT, fragmented by heat and Mg and reverse transcribed to cDNA using random primers. After 2nd strand synthesis, cDNA was then end-repaired, index adapter-ligated and PCR amplified. SPRIselect beads (# B23318, Beckman Coulter, Indianapolis, IN, USA) were used to purify nucleic acids after each step of the library preparation. RNA sequencing libraries were sequenced by the HiSeq-4000 (Illumina Inc.; San Diego, CA, USA).