Short-read metagenomic sequencing of soil: rhizosheath
300 bp fragmented DNA was used as input to prepare metagenomic libraries by using Illumina TruSeq Nano DNA Library Prep kit according to manufactures instructions. Individual libraries were pooled and the quality and quantity checked using BioAnalyzer (Agilent) and KAPA Library Quantification Kit (Roche). Pooled libraries were loaded on Illumina Novaseq6000 using 150 bp  2 paired end (PE) sequencing on S1 flow cell following the NovaSeq XP workflow