GSM5743635: granulosa_cell_1_9; Homo sapiens; RNA-Seq
Granulosa cells were collected and total RNA were extracted using Trizol reagent. Fragmented  RNA (the average length was approximately 200 bp) were subjected to first strand and second strand cDNA  synthesis following by adaptor ligation and enrichment with a low-cycle according to instructions of NEBNext®  Ultra™ RNA Library Prep Kit for Illumina (NEB, USA). RNA libraries were prepared for sequencing using standard Illumina protocols