GSM6044853: HEK293_TNRC18_KD_rescue_2; Homo sapiens; RNA-Seq
For RNA-seq, total RNA was extracted using the RNeasy Mini Kit (Qiagen #74106); in brief, RNA was first subjected for the on-column digestion by DNase (Qiagen #79254) for 0.5 hours and then prepared by using the NEBNext rRNA Depletion Kit (NEB #E6310L).