GSM5380297: Sorted intestinal crypt epithelial cells from Apln-/- adult mice, biological replicate 1; Mus musculus; RNA-Seq
Total RNA was isolated using the Qiagen RNeasy Plus Micro Kit (Qiagen) and RNA integrity was verified on a Fragment Analyzer (Advanced Analytical). mRNA was amplified using the Ovation Pico WTA System V2 (Nugen). RNA-seq libraries were prepared from 100 ng of total RNA using the Nextera DNA Flex Kit (Illumina)