GSM5532474: Sample 14- 104637 GFP- cells, Dorsal Region Organoid Fusion Day 70; Homo sapiens; RNA-Seq
Organoid Fusions were manually dissected, single organoids were kept whole. Tissue was then dissociated using a gentleMACS dissociator (Miltenyi). GFP+ and GFP- cells were then obatiend via FACS. Libraries were generated with the SmartSeq-2 protocol 2 (Illumina ). Libraries were pooled and sequenced on a HiSeq V4 flow cell (Illumina) with up to 4 billion single-end reads. We sequenced all samples twice to improve the yield and then combined the samples using samtools (htslib.org). Raw files for both sequencing runs are included in this upload.