Bicyclus anynana population resequencing
Genomic DNA was bead purified and quality was assessed on a fragment analyzer. Illumina fragment libraries were prepared by the Centre for Genomic Research (University of Liverpool) using the NEBNext Ultra II FS kit (New England BioLabs) on the Mosquito platform with 1/10 volumes (SPT Labtech) and quantified using qPCR. Libraries were sequenced over  of a lane on the Illumina NovaSeq using S4 chemistry, with 2x150 bp paired-end reads.