RNA-Seq of mus musculus: adult male inguinal subcutaneous white adipose
Total RNA was extracted using the mirVanaTM miRNA Isolation Kit (Ambion) following the manufacturers protocol. RNA integrity was evaluated using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The samples with RNA Integrity Number (RIN)  7 were subjected to the subsequent analysis. The transcriptome sequencing and analysis were conducted by OE biotech Co., Ltd. (Shanghai, China). Raw data (raw reads) were processed using Trimmomatic. The reads containing ploy-N and the low-quality reads were removed to obtain the clean reads. Then the clean reads were mapped to reference genome using hisat2. FPKM and read counts value of each transcript (protein_coding) was calculated using bowtie2 and eXpress. DEGs were identified using DESeq (2012) functions estimatSizeFactors and nbinomTest.