GSM5495163: CDX2 -/-_UiPSM_RNA-seq; Homo sapiens; RNA-Seq
Purified total RNA was capture with magnetic bead with oligo-polyA, interrupt and reverse-transcribed into cDNA using the VAHTSTM mRNA-seq V3 Library Prep Kit for Illumina (NR611, Vazyme), purified using VAHTS DNA Clean Beads (N411, Vazyme). cDNAs ligated adapters and amplified libraries using the amplification mix (NR611, Vazyme). Finally, sequencing the cDNAs library to obtain 150bp paired-end reads.