GSM5065919: 09_diABZI_6h_4; Mus musculus; RNA-Seq
Calu3 cells: Total RNA was isolated using TRIzol Reagent (Invitrogen) and purified using RNA Clean & Concentrator Kits (Zymo Research). Mice: Lung tissues were snap frozen in liquid nitrogen and homogenized with a bead beating grinder and lysis system. 900ul of TRIzol Reagent was added to 100ul of homogenized tissue. RNAseq Libraries for next-generation sequencing were prepared using the Illumina TruSeq stranded mRNA kit with Illumina TruSeq unique dual indices. Quality and quantity control of RNA and libraries were performed using an Agilent 4200 TapeStation and Qubit 4, respectively. Libraries were diluted to a final concentration of 4 nM and sequenced on an Illumina NextSeq 500 instrument to produce 75–base pair single end reads.