V1V2 amplicon from IBD patient
Amplification of the V1V2 region of the 16S rRNA gene was carried using the KAPA HiFi Hot start system (KapaBiosystems) with the primers 27F-YM and 338R-R (27F-YM: 5TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGAGTCAGTCTGTCAGAGTTTGATYMTGGCTCAG; 338R-R: 5GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGTATGGTAATTCATGCTGCCTCCCGTAGRAGT). Sample indexing was carried out with the Nextera XT Index Kit (Illumina) and library quantification and purification was carried out according to the Illumina protocol 16S Metagenomic Sequence Library Preparation. Paired end sequencing was performed using the Illumina 500 cycle MiSeq reagent kit.