TSS cappable-seq library STK005 biological replicate B2
RNA were extracted from liquid culture using a Qiagen kit, then treated with DNAse I (NEB) and purified with phenol-chlorophorm method. Cappable-seq samples were prepared following Ettwiller et al. 2016 protocol. Illumina libraries were then prepared using the NEBNext Small RNA Library Prep Set for Illumina (NEB# E7330) kit. The sequencing of the pooled libraries was performed on a Miseq apparatus.