GSM5618475: LN229_siXRN2_2; Homo sapiens; RNA-Seq
Total RNA was extracted using Qiagene's miRNeasy (cat no. 217084) using manufacturer's protocol. RNAseq libraries were constructed using the Illumina TruSeq RNA LT v2 kit and established protocols. The library construction was done using total RNA isolated from human GBM cell lines (1ug).  RNA quality for each prep was analyzed prior to construction using the Agilent Bioanalyzer 2100 and RNA nano total RNA chips.