GSM5742843: 4681-P; Homo sapiens; RNA-Seq
Blood samples (EDTA tubes) were collected from all research subjects by venipuncture. DNA and RNA were extracted simultaneously from 1ml fresh whole blood, using the AllPrep DNA/RNA Mini kit (Qiagen). Globin mRNAs were depleted from RNA samples using the GLOBINclear kit (Invitrogen). Stranded library preparation and eukaryotic transcriptome resequencing were performed using the BGISeq-500 next generation sequencing platform with paired end 100bp reads.