GSM5770147: ScACS-8-3_S46; Homo sapiens; RNA-Seq
RNA was extracted using LunaNano PureSPIN columns with a DNAse digestion step. RNA concentrations were measured using a NanoDrop One. Equal masses of RNA from 4 replicate tissues from each donor in each condition were then pooled together to reduce inter-replicate variability (12 pools in total). These samples were then shipped to the UBC Biomedical Research Center for RNA sequencing. Illumina NextSeq 500: PolyA selection, 20million paired reads