GSM5495015: scRNA-seq_wild-type2; Mus musculus; RNA-Seq
CD45+YFP+ Treg cells, CD45+YFP-CD11b- non-Treg cells and CD45+CD11b+ myeloid cells were sorted and mixed at 1:2:1 ratio from MC38 tumor and loaded onto the Chromium Controller according to their respective cell counts to target 9000 single cell GEMs (gel beads in emulsion) per sample. Libraries were prepared using the Chromium Single Cell gene expression v3.1 Library and Gel Bead Kit (10x Genomics)