GSM5769787: A375.ms023_1uM.C; Homo sapiens; RNA-Seq
RNA was extracted from cells of interest using an RNeasy kit (Qiagen, Frederick, MD) and quantified using a NanoDrop 8000 (ThermoFisher Scientific); a minimum of 500 ng of high quality RNA (as determined by Agilent Bioanalyzer) per sample or replicate was used for library preparation. Poly(A)-selected, stranded Illumina libraries were prepared with a modified TruSeq protocol and sequenced on the Illumina HiSeq 2000