Delta-p33-8mpi
Methods: A minimum of three healthy C. macrophylla plants (two years old) were graft-inoculated with buds from infected trees with CTV variants. Buds from healthy plants were used to mock-inoculated the healthy controls. All plants were maintained in a temperature-controlled greenhouse. Bark tissue samples (100 mg) from the main stem from three independent replicates of CTV- infected and healthy plants were collected at 8 months post-inoculation (mpi), and total RNA of each sample was extracted using the TRIzole reagent (Invitrogen, CA, USA) following the manufacturers protocol.  One microgram of high-quality total RNA with RNA integrity number (RIN) value above 5 was used for Library preparation and RNA-sequencing using TruSeq Stranded Total RNA Library Prep Kit (Illumina) following manufacturers instructions.