GSM5451872: SOX9 shRNA#2 (Sh2); Homo sapiens; RNA-Seq
mRNA was isolated from total RNA using NEB Next poly (A) mRNA Magnetic Isolation Module (NEB, MA, USA) and rRNA was removed using RiboZero Magnetic Gold Kit (Epicentre NY, USA). RNA library was prepared using KAPA Stranded RNA-SEQ Library Prep Kit (illumina, CA, USA) according to instruction manual prior to subject to sequencing using illumina NovaSeq 6000.