GSM5531763: Dry Eye Disease; Mus musculus; RNA-Seq
We isolated mouse corneas which were then digested first with enzyme G (Miltenyi 130-095-928) to separate the epithelium. Then, 0.25% trypsin was used to dissociate the epithelial sheets into single cells. We constructed libraries using the Chromium Next GEM Single Cell 3ʹ Kit v3.1, according to the manufacturer's recommended protocols.