GSM5770413: CD49a_pool1; Mus musculus; RNA-Seq
Leukocytes were isolated from kidneys by digestion, percoll gradient, erythrocyte lysis and CD45 enrichment using CD45-microbeads (Miltenyi). After sorting of CD49bhiNK1.1+NKp46+CD3- and CD49a+CD49blow/intNK1.1+NKp46+CD3- cells, total RNA was extracted using RNAeasy MicroKit (Qiagen). 250-300 bp cDNA insert library, 6G raw data/sample on average