2bRAD of adult Ostrea lurida adductor muscle
For the 2b-RAD reduced-representation sequencing approach, the frequent-cutter restriction enzyme AlfI was used with modified adaptors (5-NNR-3) to target  of all AlfI restriction sites in the genome. We followed the 2bRAD library protocol developed by Eli Meyer (available at https://github.com/sr320/paper-oly-mbdbs-gen), except that we used 900 ng of starting DNA, 19 PCR cycles as determined by a test PCR, and we concentrated the final pooled libraries using a Qiagen PCR kit prior to sequencing.