GSM5494229: omniATAC-seq_Melanophore, rep2; Danio rerio; ATAC-seq
Single cells were dissociated from embryos using Gibco TrypLE Express enzyme solution. Neural crest GFP-positive cells were sorted and collected on Beckman Couter MoFlo. Melanophore and iridophore were isolated using published protocol developed by the Johnson lab (Higdon et al. 2013). Genomic DNA for WGBS was purified from NCCs and pigment cells via phenol-chloroform:isoamyl alcohol (PCI) extraction and ethanol precipitation method. RNA was collected using Trizol following manufacturer's recommendation. Published omniATAC-seq protocol was followed to extract materials for ATAC-seq. RNA-seq libraries were then constructed with TruSeq RNA Library Prep Kit v2 (Illumina, RS-122-2001) following manufacturer's instructions. gDNA was bisulfite treated using EZ DNA Methylation-Direct kit (Zymo, D5020) and processed with TruSeq DNA Methylation Kit (Illumina, 15066014) to generate Illumina-compatible WGBS libraries. Published omniATAC-seq was followed to generate ATAC-seq data.