Bartonella ssrA amplicon from Ctenocephalides felis HH-13-1
Illumina overhang adapters were added to two sets of amplicon primers to amplify a ~301 bp and ~360 bp region of the ssrA and gltA genes of Bartonella, respectively. The ssrA and gltA conventional PCR reactions were performed with Q5 Hot Start High-Fidelity 2X Master Mix (New England Biolabs, Australia). The ssrA and gltA PCR amplicons were submitted for NGS at the Ramaciotti Centre for Genomics, University of New South Wales, Australia for sample preparation and paired 250 bp reads were sequenced on an Illumina MiSeq system.