GSM5285463: FLAG-MITF-A-input; Homo sapiens; ChIP-Seq
The cells were collected by scraping and were lysed with lysate buffer, the nuclear pellet was digested by micrococcal nuclease. Immunoprecipitation was performed on the lysate with 2μg of anti-FLAG antibody The DNA fragments were then sequenced using the Illumina HiSeq X-ten platform.