GSM5494835: BBB model replicate 3; Homo sapiens; RNA-Seq
Cells were lysed by Trizol reagent followed by addition of chloroform. After centrifuge, the aqueous phase was obtained and ispropyl alcohol was added to the aqueous phase to precipitate the RNA. The RNA pellet was washed by 75% ethanol and the dissolved in RNase-free water. RNA libraries were prepared for sequencing using standard Illumina protocols