GSM5831374: WT_VA24_#2; Homo sapiens; RNA-Seq
The cells were harvested in TRIpure reagent (Roche, cat. no. 11667165001) for total RNA isolation followed by on-column Dnase I treatment with miRNeasy Mini kit (Qiagen, cat. No. , 217004). Total RNA-seq libraries were prepared with Illumina TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero ribosomal RNA reduction chemistry.