GSM5144825: wt_5; Caenorhabditis elegans; RNA-Seq
worms were placed in M9 and 20 intestines per sample were dissected out and flash frozen in 1mL Qiazol. Total RNA was extracted using the miRNeasy Micro kit (Qiagen) according to manufacturer's protocol. Smart-seq2  was used for the generation of non-strand-specific, full transcript sequencing libraries using standard reagents and procedures as previously described (Picelli et al, 2014)