GSM5896942: RNA-seq Dppa3 KO female E13.5 rep3; Mus musculus; RNA-Seq
Total RNA was extracted from PGCs using Trizol reagent (Thermo Fisher Scientific). WGBS (PBAT) libraries were constructed as previously described (Miura et al., 2012; Au Yeung et al., 2019). RNA-seq libraries were constructed using NEBNext rRNA Depletion Kit, NEBNext Ultra II Directional RNA Library Prep Kit for Illumina, and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (NEB).