Paraclostridium bifermentans strain:DSM 14991 Genome sequencing
Genomic DNA libraries are prepared using the Nextera XT Library Prep Kit (Illumina, San Diego, USA) following the manufacturers protocol with the following modifications: input DNA is increased 2-fold, and PCR elongation time is increased to 45 s. DNA quantification and library preparation are carried out on a Hamilton Microlab STAR automated liquid handling system (Hamilton Bonaduz AG, Switzerland). Pooled libraries are quantified using the Kapa Biosystems Library Quantification Kit for Illumina. Libraries are sequenced using HiSeq 2500 using a 250bp paired end protocol. Reads are adapter trimmed using Trimmomatic 0.30 with a sliding window quality cutoff of Q15