Homo Sapiens Rheumatoid Arthritis CD4 Central Memory T cell ATAC-seq
ATAC-seq libraries were generated on CD4 central memory T cells followed by standard ATAC-seq protocol described previously. Briefly, 50,000 sorted T cells were washed with cold PBS and RSB buffer (10mM Tris-HCl (pH 7.4), 10mM NaCl, 3mM MgCl2) followed by washing with RSB buffer containing 0.1% NP-40 and 0.1% Tween 20. Cell pellet were resuspended in the transposase reaction mix (25L 2 TD buffer, 2.5L transposase (Illumina) and 22.5L nuclease-free water) and incubated at 37 C for 30 min. Fragmented genomic DNA was further purified with a Qiagen MiniElute kit and library amplification was performed using Nextera PCR primers. The quality of the libraries were confirmed by bioanalyzer and sequenced on an Illumina NextSeq 500 by Stanford Functional Genomics Facility.