GSM4978639: 0.6mM As (III) treated rep 1; Xanthomonas oryzae pv. oryzae; RNA-Seq
The cells were harvested by centrifugation at 6,000g for 5min for RNA isolation and then lysed using Trizol reagent. Total RNA was isolated using Direct-zol RNA MiniPrep kit (Zymo Research Corporation, Orange, CA, USA) The rRNA was removed using 'si-tools Pan-prokaryote ribopool probes,' and further library preparation was done using TruSeq Stranded RNA library prep kit. Prepared libraries were sequenced using Illumina HiSeq x10 platform, and 150-bp paired-end reads were generated.