Amplicon sequencing of the Arbuscular Mycrrhizal Fungi SSU 18S rRNA: Manzanillo.Autumn
The intraradical AMF communities were analysed via Illumina MiSeq 2x300 bp pair-end sequencing, using a semi-nested approach targeting the SSU region of the 18S rRNA gene. The Glomeromycota-specific AML1-AML2 primer pair (Lee et al., 2008) was used initially, followed by WANDA-AML2 (Dumbrell et al., 2035). PCR products were purified using calibrated Ampure XP beads and the purified products were used to prepare the DNA libraries by following the Illumina TruSeq DNA high-throughput library preparation protocol. DNA library preparation and sequencing was performed at Mr. DNA (www.mrdnalab.com, Shallowater, TX, USA) on a MiSeq platform following the manufacturers guidelines.