GSM5993859: Trem2_mut_MDSCs_rep2; Mus musculus; RNA-Seq
RNA was then extracted using TRIzol RNA isolation reagent (Invitrogel, Cat n=15596026) and RNase-Free DNase set (Qiagen, Cat n=79254) treatment was performed following manufacturers' guidelines. RNA integrity was checked with Agilent 2100 Bioanalyzer system. Protocol used was NEBNext rRNA depletion KIT (Human\Mouse\Rat) (“NEB #E6310”). Sequencing of the libraries was performed using the NextSeq 500 with the NextSeq 500/550 High Output Kit v2 (75 cycles; Illumina).