GSM4850871: ATAC_KO27_2_r3; Mus musculus; ATAC-seq
The OmniATAC transposition reaction was performed with 50 000 cells as previously described (Corces, Trevino et al. 2017) using the Tagment DNA TDE1 Enzyme (Illumina, 20034197). DNA was purified using the PCR clean-up MinElute kit (Qiagen, 28006). The transposed DNA was subsequently amplified in 50µl reactions with custom primers as previously described (Buenrostro et al., 2013). Libraries were purified and size selected for fragments less than 600bp using the Agencourt AMPure XP beads (Beckman Coulter, A63882).