GSM5573159: 5_DDC28; Mus musculus; RNA-Seq
RNA was extracted from 20 mg mouse liver tissue preserved in RNAlater (ThermoFisher Scientific, Belgium) using the Aurum Total RNA Mini Kit (Bio-Rad Laboratories, Belgium), following manufacturer's protocol. Quantification and purity of the isolated RNA were determined via spectrophotometric analysis with a Nanodrop spectrophotometer (ThermoFisher Scientific, Belgium). Library construction was performed with the QuantSeq 3' mRNA FWD kit (Lexogen) and UMI Second Strand Synthesis Module (Lexogen).