GSM5618382: TA - Immobile scRNA-seq; Mus musculus; RNA-Seq
TAs were pooled according to treatment (Mobile vs. Immobile). The cleaned muscles were minced and then enzymatically digested for ~60 minutes at 37°C. The tissue solution was filtered (70 μm and 40 μm) to obtain mononuclear cell suspension, and the filtrate was centrifuged at 300 RCF for 5 minutes. Supernatant was decanted, and cell pellet was resuspended in PBS with 5% FBS. Two single-cell libraries (one for the pooled mobile TAs and the other for the pooled immobile TAs) were constructed with the Chromium Single-Cell Gene Expression Kit Version 3 and sequenced on two 2x150nt SP lanes in a NovaSeq 6000.