GSM5380739: lncRNA-Smad7-KIpA-2-ES-1; Mus musculus; RNA-Seq
Total RNA was extracted by commerical RNA extraction Kit. Lysates for ChIP and ChIRP assays were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody or antisense probes. RNA libraries were prepared for sequencing using standard Illumina protocols, and the libraries were sequenced on Illumina HiSeqXten-PE150 following the manufacturer's protocols. For ChIP-seq and ChIRP DNA-seq, libraries were prepared according to Illumina's instructions and the libraries were sequenced on the Illumina HiSeq-PE150 following the manufacturer's protocols.