GSM5287299: ChIP antiH4K5bu REH WT; Homo sapiens; ChIP-Seq
For sequencing, ChIP libraries were prepared using MicroPlex Library Preparation Kit v2 (Diagenode) according to manufacturer's instructions. Each library was quantified on Qubit with Qubit® dsDNA HS Assay Kit (Life Technologies) and size distribution was examined on the Fragment Analyzer with High Sensitivity NGS Fragment Analysis kit (Agilent). ChIP libraries against H4K5bu and BRD4 were sequenced using the NovaSeq 6000 in PE150 mode according to manufacturer's instructions at the Novogene company.