RNA-Seq of human
Total RNA was extracted with TRIzol reagent (15596018, Thermo Fisher Scientific) and then reverse transcribed to cDNA with a Prime Script RT Master Mix Kit (RR036A, TaKaRa, Tokyo, Japan). The resulting cDNAs were analysed by Q-PCR using an GoTaq qPCR Master Mix (A6002, Promega) in a LightCycler 480 instrument (Roche Diagnostics, Switzerland) and the data were normalized to -Actin, as an endogenous control. Relative gene expression was analyzed using the 2Ct method. The primers used in this study were synthesized by TSINGKE Biological Technology (Guangzhou, China) and are listed in Supplementary Table S4.