NextSeq 550 sequencing; RNA-seq of RpoD ortholog mutants from X manuscript
RNA-seq of RpoD ortholog mutants from X manuscript
Samples were harvested during mid-log growth phase. Samples were cultivated in culture tubes with LB and appropriate antibiotics and harvested at mid-log phase (approx. OD600 0.5). Total RNA were extracted from the cells using the RNA-snap protocol. RNA libraries were prepped using NEBnext ultra directional RNA library kit.