GSM5380712: input-hdac4_rep2; Mus musculus; ChIP-Seq
Cells were lysed with buffer and sonicated. After preclearing chromatin extract, anti-Hdac4 and anti-Hdac5 antibodies were used to immunoprecipiate in mESCs respectively. Proteins on chromatin associated with Hdac4/5 were digested and chromatin was decrosslinked at 65 ℃ overnight. DNA was extracted in phenol-chloroform. For ChIP-seq, 5ng of Hdac4/5-assocated chromatin DNA was used in ChIP-seq library preparation.  Libraries were prepared according to Illumina protocols and library fragments of ~250 bp were used for sequencing.