14_Cas_a8_No_Target_1_VEGFA2
PCR was performed in two rounds to amplify the genomic DNA region surrounding each target site and add on the sequences required for Illumina sequencing and indexing (Svitashev et al., 2015; Karvelis et al., 2015). DNA was then purified using a Monarch PCR purification column (NEB) or Zymoclean Gel DNA Recovery Kit (Zymo Research), combined in an equimolar fashion, and sequenced on a MiSeq System (Illumina) with custom sequence primers, one for the amplicon and the second for the index.