GSM4851096: HMLE KO ML rep2; Homo sapiens; RNA-Seq
RNA was isolated with Trizol-Chloroform followed by isopropanol precipitation. RNA-Seq Libraries for HMLE cells were prepared by the Institut Curie Next Generation Sequencing Core Facility using the TruSeq Stranded mRNA kit (Illumina, ref. 20020594-5) according to manufacturer's instructions and sequenced on an Illumina HiSeq 2500 (PE100). RNA-Seq libraries for HMLER cells were prepared using the Swift RNA Library Kit (Swift Biosciences, ref. R1024 and R1096) according to manufacturer's instructions and sequenced on an Illumina NovaSeq 6000 (PE100).