GSM6259829: hu14.18-IL21, scRNAseq; Mus musculus; RNA-Seq
Neuroblastoma tumors were freshly processed into single cell suspensions and enriched via CD45 magnetic bead positive selection (Stem Cell Technologies). The viability of samples was confirmed to be >80% prior to further processing. Three individual mouse samples per treatment group were pooled. About 10,000 cells from each treatment group were loaded with the goal of capturing 6,000 cells using the 10X Genomics 3' v3.1 chemistry. We followed the manufacturer's instructions for library generation. The cDNA libraries were sequenced on the NextSeq2000 with a target depth of approximately 50,000 reads per cell.