16S rRNA gene sequencing of a filtered water sample: 0.2 µm to 2 µm particles
DNA extracted using DNeasy PowerWater Sterivex kit. v4 region of 16S rRNA gene amplified in triplicate reactions. Triplicates pooled and analyzed using a QIAxcel. Samples then pooled, cleaned using the Generead Size Selection kit, and diluted for loading onto Illumina Miseq