Illumina HiSeq 2500 sequencing; RNA-seq of human monocytic cell line (THP-1) treated with compound 81 or vehicle controls
RNA-seq of human monocytic cell line (THP-1) treated with compound 81 or vehicle controls
THP-1 cells were cultured with 5 uM compound 81 or 0.5% DMSO. 5 h later the cells were lysed in Trizol for RNA extraction. Total RNA was extracted from Trizol using the miRNeasy Mini Kit (Qiagen, 217004) according to the manufacturer's instructions. Biological triplicates were obtained for each genotype.  500ng of each sample's total RNA was then prepared into barcoded mRNA libraries, according to manufacturer's instructions, using the Truseq Stranded mRNA Library Prep Kit (Illumina, RS-122-2103).