Illumina NovaSeq 6000 paired end sequencing; Effect of 20-Hete and dietary restriction on murine kidneys
Effect of 20-Hete and dietary restriction on murine kidneys
Undamaged murine kidneys were withdrawn after anesthesia by ketamin/xylazine. RNA isolation of total RNA was performed using commercial homogenization (Bio 101 FastPrep FP120-120V, Savant, Midland, MI) and the RNeasy mini kit (QIAGEN, Hilden, Germany). RNA sequencing was done by the Cologne Center for Genomics using the Ribo-Zero Human/Mouse/Rat preparation kits and the TruSeq Stranded Total RNA samples (Illumina Inc., San Diego, CA). Library preparation was done by the Cologne Center for Genomics using the TruSeq Stranded mRNA Library Prep Kit with an additional ERCC-spike-in.