Whole exome sequencing of Colo205 cell line
Genomic DNA was fragmented by sonication, adaptors ligated, and fragments amplified by PCR. Fragment libraries were then hybridized with biotinylated probes (Agilent SureSelect Human All Exome V6) and pulled down with streptomycin-coated magnetic beads to enrich for exonic fragments. Captured fragment libraries were then amplified and index tagged in a PCR reaction followed by purification by AMPure XP beads.