GSM5495196: 19_Skin_CompoundHetero_rep1; Mus musculus; RNA-Seq
After euthanasia, back skins were removed from palm and dissociated into single cells by the Liberase enzyme. RNA-seq library was constructed using a modified smart-seq2 protocol. Cells were sorted into 384-well plates with barcoded oligo dT primer for each well (1 cell / well). After reverse transcription in each well, cDNA was pre-amplified using the Accel-NGS 1S Plus DNA Library Kit (Swift Biosciences, Ann Arbor, MI, USA). Purified cDNAs were tagmentationed with Amplicon Tagment enzyme (Illumina) and PCR for library construction was done by using NEBNext High-Fidelity 2× PCR Master Mix (NEB).