GSM5967698: PHA_GD21_Ctrl_18-22_25b; Rattus norvegicus; RNA-Seq
Dams were exposed 1 h ± 15 min before decapitation under CO2/O2-anesthesia on GD17 or 21. For gene expression analyses, the perineum including the genital tubercle (GT) was more finely dissected and placed in RNAlater. Total RNA was extracted (n = 11-12 samples/group) using an RNeasy Micro Kit (Qiagen, Germany) according to manufacturer's instructions, including on-column DNase I digestion. The sequencing libraries were built by tagmentation using 50 ng of ds cDNA with the Illumina Nextera XT Kit (Illumina, #FC-131-1024) following the manufacturer's recommendations.