s20160414C1
A total amount of 1.5g DNA per sample was used as input material for the DNA sample preparations. Sequencing libraries were generated using Truseq Nano DNA HT Sample preparation Kit (Illumina USA)following manufacturers recommendations and index codes were added to attribute sequences to eachsample. Briefly, the DNA sample was fragmented by sonication to a size of 350bp, then DNA fragmentswere end polished, A-tailed, and ligated with the full-length adapter for Illumina sequencing with furtherPCR amplification. These libraries constructed above were sequenced by Illumina NovaSeq 6000 platform and 150bp paired-endreads were generated with insert size around 350bp.