16S of  Gallus gallus: growing hen cecal contents
Microbiome DNA extracted from fecal samples of the cecum, the primers were designed according to the conserved region, and the sequencing junction was added at the end of the primers to perform PCR amplification was performed and the products were purified, quantified and homogenized to form a sequencing library. The libraries were sequenced with Illumina Novaseq 6000.