GSM5531728: Wild-type petunia flowers - Rep 3; Petunia x hybrida; RNA-Seq
Total RNA was isolated from at least eight petunia corollas harvested at 20:00 h on day 2 post-anthesis using the RNeasy Plant Mini Kit (Qiagen), treated with DNase I to eliminate genomic DNA using the TURBODNA-free kit (Ambion, Foster City, CA, USA) and was reverse-transcribed to cDNA using the High Capacity cDNA reverse transcription kit (Applied Biosystems, Foster City, CA, USA). RNA libraries were prepared for sequencing using standard Illumina protocols