GSM5380096: CR5_C3_24h; Spathaspora passalidarum; RNA-Seq
For cell lysis, the tubes containing the cell suspension samples in TRIzol™, 200 µL of 0.5 mm GlassBeads (Sigma-Aldrich, Darmstadt, Germany) was added. The Eppendorf tubes were shaken in 05 cycles lasting 60 s alternating with ice cooling for 60 s at a speed of 5 m.s-1 per cycle in a FastPrep 24 bench homogenizer (MP Biomedicals, Irvine, CA, USA).  Total RNAs were extracted from the cells of each sample, using the Direct-zol ™ RNA MiniPrep Plus extraction kit (Zymo Research, Irvine, CA, USA), according to the manufacturer's instructions. RNA libraries were prepared for sequencing using standard Illumina protocols