GSM5949448: CT_4; Homo sapiens; RNA-Seq
Following treatment on day 21, xenografts were removed, flash frozen in liquid nitrogen, and RNA was extracted using the Monarch Total RNA Miniprep Kit (NEB, #T2010) following manufacturer's protocols. RNA quality was assessed on the Agilent Bioanalyzer 2100 system. A total amount of 1 µg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEB Next® Ultra RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations. Library quality was assessed on the Agilent Bioanalyzer 2100 system. Library preparations were sequenced on an Illumina NovaSeq 6000 PE150 system and paired-end reads were generated.