GSM5066056: WT rep2 [ATAC-seq]; Mus musculus; ATAC-seq
A total of 50,000 cells were washed once with 50 µl of cold PBS and resuspended in 50 µl lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% (v/v) NP-40). To perform transposition reaction, the collected nuclei by centrifuged for 10 minutes at 500g at 4°C was mixed with the transposition reaction mix (10 µl TTBL, 5 µl TTE Mix V50 and nuclease-free H2O up to 50 µl in total) of TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme, catalog. no. TD501-01) for 10 minutes at 55 °C. Samples were then PCR amplified. Post-PCR clean-up was performed by adding 1.2 volume of DNA clean beads (Vazyme, catalog. no. N411-02). Library concentration was measured with Qubit. Library integrity was checked by the Agilent bioanalyzer. Finally, the ATAC library was sequenced on an Illumina Hiseq X10 platform and 150 bp paired-end reads were generated.