GSM5832818: Control rep5; Danio rerio; RNA-Seq
Total RNA was extracted and purified with TRIzol (Invitrogen, United States) as described by the manufacturer. RNA quantity and purity was determined with the NanoDrop ND-1000 (NanoDrop, Wilmington, United States) and Bioanalyzer 2100 (Agilent, USA). Only RNAs with a high RNA integrity number (RIN > 7) were selected and used for the subsequent sequencing. RNA libraries were prepared for sequencing using Illumina Novaseq™ 6000