Reh cell line, IGF2BP1 knockout, replicate 1
RNA sequencing and library preparation was performed by the UCLA Technology Center for Genomics and Bioinformatics with Kapa hyper Prep Kits with RiboErase. The workflow consists of rRNA depletion, cDNA generation, and end repair to generate blunt ends, A-tailing, adaptor ligation and PCR amplification. Different adaptors were used for multiplexing samples in one lane. Single-end, strand-specific RNA sequencing was performed on the Illumina HiSeq3000 system resulting in 30-50 million reads per sample.