GSM5064792: luminal cell-100; Mus musculus; RNA-Seq
The 3D culture of basal and luminal cells was harvested. Total RNA was extracted using RNAiso plus (Takara). mRNA was then enriched by oligo (dT) beads. RNA sequencing libraries were generated using the KAPA Stranded RNA-Seq Kit for illumina with multiplexing primers, according to the manufacturer's protocol. RNA libraries were prepared for sequencing using standard Illumina protocols