GSM5570255: MCF7 cells, shRNACOP1-2-5h; Homo sapiens; RNA-Seq
Total RNAs were extracted using Trizol. RNA sequencing was performed on libraries prepared from total RNA samples. Three biological replicates were analyzed for each condition. RNA integrity was verified on a Bioanalyser 2100 with RNA 6000 Nano chips (Agilent technologies, CA, USA). RNA integrity number score was above 9 for every sample Libraries were prepared using Truseq® stranded mRNA Sample Preparation Kits (Illumina, CA, USA) following manufacturer's instructions. Libraries were validated using QIAxcel Advanced System and quantified by qPCR using the KAPA library quantification kit. Libraries were multiplexed and sequenced on an Illumina NextSeq500 sequencer to generate more than ~25,000,000 paired-end reads (2 × 150 bases) per library.