GSM6195528: PC3 TRIM29 overexpression rep2 [PC3_OE_TRIM29_2]; Homo sapiens; RNA-Seq
Isolation of RNA from cells was performed using the Lira reagent (Biolabmix, Russia) according to the manufacturer's protocol. Library preparation was performed with NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit (NEB) according to the manufacturer's protocol. The library underwent a final cleanup using the Agencourt AMPure XP system (Beckman Coulter) after which the libraries' size distribution and quality were assessed using a high sensitivity DNA chip (Agilent Technologies). Libraries were subsequently quantified by Quant-iT DNA Assay Kit, High Sensitivity (ThermoFisher).