AMPLICON of gut metagenome: adult human
DNA was extracted from 200 ul sample, using the PowerMag Soil DNA Isolation Kit optimized for epMotion and the epMotion robotic platform model 5075vt. Two-step PCR of the v3-v4 region was performed, First 30 cycles with broad range primers (Uni341F and Uni806R), then 15 cycles to add sequencing primers and adaptors. 250 bp Paired-end sequencing, of up to 192 samples, were performed on the Illumina MiSeq System using MiSeq Reagent Kits v2