Whole Genome Sequencing of human DNA: Sample SL262929
Genomic DNA was isolated from whole blood (peripheral blood leukocytes) using the PAXgene system (PreAnalytiX) or DNeasy Blood and Tissue kit (Qiagen). Sequencing libraries were constructed using 2.5 ug of genomic DNA according to a modified version of the Illumina library construction protocol. For whole genome sequencing, libraries were sequenced on the Illumina HiSeq X to obtain paired-end 150 bp reads, with mean coverage depth of 35X with 80% of bases covered at 20X.