16S rRNA V4 region of bird metagenome from Grus grus sample 518
DNA was extracted from a fecal sample of the crane.  Then the V4 region of the 16S rRNA gene was amplified using the primers 515F and 806R.  Three independent PCRs were performed for each sample, which were then combined. Equimolar amounts of each sample were then pooled before sequencing on a 151 bp paired-end run of an Illumina MiSeq.