Shotgun metagenome (forward reads) of human gut microbiome: stool: Sample 2959BY_MG
"We used the DNeasy PowerSoil Kit (QIAGEN) with manufacturers' protocols to extract microbial genomic DNA from stool samples. Genomic DNA libraries were constructed from 1.2ng of cleaned DNA using the Nextera DNA Library Preparation kit (Illumina) according to the manufacturer's recommended protocol, with reaction volumes scaled accordingly. Prior to sequencing, libraries were pooled by collecting equal quantity of each library from batches of 94 samples. Insert sizes and concentrations of each pooled library were determined using an Agilent Bioanalyzer DNA 1000 kit (Agilent Technologies). Paired- end sequencing (2x150-bp reads) was performed using an Illumina NextSeq 500 instrument (Illumina Inc) at the Broad Institute."