GSM5570608: HSPCs, HLA-DR(-) rep2; Homo sapiens; RNA-Seq
HLA-DR(+) HSPCs and HLA-DR(-) HSPCs were isolated by magnetic cell separation and fluorescence-activated cell sorting. SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Bio USA, San Jose, CA) was used for the construction of sequencing libraries. cDNA libraries were prepared for sequencing using Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA) and Nextera XT Index Kit v2 set A/B/C/D (Illumina, San Diego, CA).