GSM5831735: Large_intestine_high_R1; Mus musculus; RNA-Seq
Small and large intestinal crypts were treated with TrypLE supplemented with DNaseI and Y-27632. Dissociated cells were filtrated, resuspended and incubated with antibodies in PBS. Dead cells were eliminated and Lgr5+ cells were sorted by positive staining for Epcam and endogenous Lgr5-GFP signal. RNA was isolated using RNeasy Micro Kit (Qiagen) with 270 pg or more of RNA. RNA libraries were prepared for sequencing using SMART-Seq Stranded Kit (Takara) and sequenced as paired end (2x100bp) using NovaSeq 6000.