GSM5379774: LEO1 ChIP-seq in control hESCs replicate 1 (LEO1-H9-1); Homo sapiens; ChIP-Seq
Crosslinked DNA-proteins were fragmented with ChIP-IT® Express Enzymatic Shearing Kit (Active Motif),cell lysates were further subject to IP with antibodies targeting specific protein. Iped DNA-protein were de-crosslinked, DNA was purified and subjected to library preparation. ChIP-seq library preparation kit (Vazyme, catlog: ND607-01) was used for preparation of ChIP-seq libraries. Libraries were sequenced with Novaseq PE150