GSM6303978: GRA-Dex-1 [hippocampus]; Mus musculus; RNA-Seq
The hippocampus was collected after treatment and total RNA isolated using Qiagen RNeasy mini kit. The integrity of the total RNA was determined using a bioanalyzer (Agilent) and RNA concentration was assessed using a Qubit (ThermoFisher Scientific). A TruSeq RNA kit (Illumina, San Diego, CA) was utilized to prepare the poly(A)-enriched RNA-seq libraries that were sequenced on the Illumina NovaSeq 6000 in a 75-base paired-end mode according to the manufacturer's protocol.