GSM5966028: DSP-1001660003872-E-E05; Homo sapiens; OTHER
In brief, we sectioned clinical FFPE blocks at 5 um onto SuperFrost Plus microscopy slides and then followed the manufacturer instructions for RNA (NanoString) with minor modifications as noted in the associated manuscript. Libraries were constructed according to manufacturer protocols (NanoString) for sequencing on a NovoSeq S2 (Illumina) with the following paired end read configuration: read 1, 27 nt; read 2, 27 nt; index 1, 8 nt; index 2, 8 nt.