RNAseq of Leucoraja erinacea pharyngeal arches
Samples were preserved in RNAlater, total RNA was extracted using the RNAqueous-Micro Total RNA Isolation Kit (ThermoFisher), and library prep was performed using the Nextera XT library prep kit. S23/24 and S25/26 libraries were pooled and sequenced using the HiSeq4000 platform (paired end sequencing, 150bp read length) at the CRUK genomics core facility (University of Cambridge, Cancer Research UK Cambridge Institute). In addition to the above, libraries from the dorsal mandibular arch (n=5), ventral mandibular arch (n=5), dorsal gill arch (n=5) and ventral gill arch (n=5) domains of S29 skate embryos were prepared as described above, and sequenced using the NovaSeq 6000 (paired end sequencing, 150bp read length) at Novogene Co., Ltd.