GSM4978180: sRNA C. elegans adult ebax-1 rep 3; Caenorhabditis elegans; miRNA-Seq
Worms were homogenized using a TissueLyser II with glass beads.  RNA was harvested using TRI-Reagent. Small-RNA sequencing libraries were prepared as described (Kingston and Bartel, 2019).  A step-by-step protocol is available at http://bartellab.wi.mit.edu/protocols.html. Libraries were prepared from 5 ug total RNA extracted from worms.