GSM4848256: whole gut total RNA- st95 het1; Danio rerio; RNA-Seq
Toal RNA extracted by using the RNAqueous-Micro Total RNA Isolation Kit (Ambion). Total RNA was used to create cDNA libraries with adapters using the KAPA stranded mRNA-seq kit. Magnetic oligo-dT beads were used to capture mRNAs. Amplification of libraries was conducted using high fidelity, low bias PCR.