GSM4849482: RNA-Seq of Corynebacterium glutamicum C1, untreated control, independet replicate 3; Corynebacterium glutamicum; RNA-Seq
RNA was extracted with the NucleoSpin RNA isolation kit (Macherey-Nagel, Düren, Germany) according to the manufacturer's instructions. Residual DNA was removed by digestion using the RNase-free DNase set (Qiagen, Hilden, Germany).  After DNA digestion, the RNA was again purified with the same kit. RNA quality was checked by Trinean Xpose (Gentbrugge, Belgium) and the Agilent RNA 6000 Nano Kit on an Agilent 2100 Bioanalyzer (Agilent Technologies, Böblingen, Germany). Ribosomal RNA molecules were removed from total RNA with the Ribo-Zero rRNA Removal Kit bateria (Illumina, San Diego, USA) and removal of rRNA was checked with the Agilent RNA 6000 Pico Kit on an Agilent 2100 Bioanalyzer. Libraries of cDNA were prepared with the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, USA). The resulting cDNA was sequenced in a paired end run on an Illumina HiSeq 1500 system in a 2 x 70 nt run.