WGS of Paracoccidioides brasiliensis
About 1g of DNA was fragmented and used as input to prepare DNA paired-end libraries using the KAPA HTP Library Preparation Kit. We used the KAPA Library Quantification Kit Illumina Platforms (Roche) to quantify the libraries via qPCR and then pooled them in a single Illumina flow cell. Sequencing was performed in an Illumina NextSeq 550 system (Illumina) using a High Output Kit v2 (300 cycles), 2 X 150bp.