Enhancement of PHA production by a mixed microbial culture using VFA obtained from the fermentation of wastewater from yeast industry
pyrosequencing (Keskin et al., 2017) utilizing Roche 454 FLX titanium instruments and reagents following manufacturers guidelines (MR DNA, Shallowater, TX, USA). The eubacteria 16S primers 27F (5GAGTTTGATCNTGGCTCAG-3) and 519R (5GTNTTACNGCGGCKGCTG-3) used amplified a fragment of approximately 500 pb.