GSM5285845: RRBS MommeD43 rep2; Mus musculus; Bisulfite-Seq
Genomic DNA wasextracted using a Qiagen Dneasy kit. RRBS was performed with 100ng of genomic DNA from each sample of female primary Smchd1MommeD43/MommeD43 and Smchd1+/+ NSCs (3 independent cell lines of each genotype) with the Ovation RRBS Methyl-seq kit (NuGen) following the manufacturer's instructions. The bisulphite conversion was performed with the EpiTect Fast Bisulfite Conversion kit (Qiagen) following the instructions.