GSM4848637: CRISPR_KO_Ctrl_ATAC_seq_rp1; Mus musculus; ATAC-seq
WT and GPS2 KO RAW264.7 cells were seeded with triplicates in the 12 wells plates with 2×105 cell number one day before the experiments. Cells were harvested (basal condition), washed with PBS and then resuspended in lysis buffer. Cell nuclei were spined down and transferred into the transposition reaction buffer and incubated at 37 °C for 30 minutes. Genomic DNA was extracted using Qiagen MinElute PCR Purification Kit. ATAC-seq library was done with the protocol as  previously reported (Buenrostro et al., 2015). The Purified ATAC-seq DNA library mix was sequenced on NextSeq 550 (Illumina, 75 SE reads) in BEA Core Facility (Karolinska Institutet, Huddinge, Sweden). DNA was processed using Takara SMRTer ThruPLEX DNA-seq Kit.