GSM4978004: StAgo1-GFP H2O 2; Solanum tuberosum; miRNA-Seq
Inoculated leaves were harvested and flash frozen in liquid nitrogen. StAGO1a-GFP co-IP were performed with anti-GFP antibody (Chromotek, gtm-100). Barcoded sequencing libraries were constructed using the Ion Total RNA-Seq Kit v2 and Ion Xpress barcode primers. Sequencing was performed on the Ion Proton platform using the Ion PI™ Hi-Q™ Sequencing 200 Kit (Thermo Fisher Scientific).