The complete chloroplast genome of Osteospermum ecklonis
After testing the genomic DNA of the sample, the DNA was interrupted by physical method (Chao Shengbo), and then the DNA after interruption was purified and the sequencing library was constructed. The steps were as follows: DNA terminal repair, 3 'end plus A, joining the sequencing connector, agarose gel electrophoresis to recover the target fragments, PCR amplification of the target fragments, and finally establishing the sequencing library. Before sequencing, the constructed library was subject to quality inspection. After passing the quality inspection, it was sequenced on Illumina novaseq6000 platform