ChIP-seq of histone mark H3K27me2me3 in Drosophila simulans Sz11 female head tissue under no ethanol control treatmen
Flies were flash frozen and heads isolated on dry ice. Approximately 200 fly heads underwent chromatin immunoprecipitation. Two aliquots were collected for H3K4me3 and H3K27me2me3 immunoprecipitation and ChIP-seq library preparation to generate an indexed library for each mark. Libraries were quantified and pooled for sequencing. The final pooled library was sequenced on one lane at UF ICBR (Gainesville, FL).