GSM5993866: E13.5, H3K27ac; Mus musculus; ChIP-Seq
Chromatin was prepared from E13.5 and P0 NPCs using the Diagenode iDeal ChIP-seq kit for Histones. Samples corresponding to 0.5 million cells were resuspended in 100 µl of shearing buffer iS1 and sheared during eight cycles of 30 s 'ON'/30 s 'OFF' with the Bioruptor Pico combined with the Bioruptor Water cooler.The shearing efficiency was analyzed using an automated capillary electrophoresis system Fragment Analyzer (High sensitivity NGS fragment kit) after RNAse treatment, reversal of the crosslinking and purification of DNA. 500 pg of DNA was  used for library preparation using the MicroPlex v2 protocol. The ChIP samples (eight samples in total) were processed together. A control library was processed in parallel of the samples using the same amount of a control Diagenode ChIP's DNA. 12 cycles of amplification were performed to amplify the libraries. After amplification, 1 µl of each library was loaded on BioAnalyzer for quality and quantified using the Qubit ds DNA HS kit.