GSM5448683: Treated tumor draining LN mouse #1; Mus musculus; RNA-Seq
Two days after the day of cyclophosphamide, tumor-draining inguinal LNs were harvested from all mice, mechanically disaggregated, passed through 40 um mesh strainer, and washed 4 times with PBS + 1% BSA.  Cells were processed for scRNAseq libraries using the Chromium Controller (10X Genomics). scRNAseq libraries were generated for approximately 2,000 cells per sample using 10X 3' single cell mRNAseq V3 reagents.  The scRNAseq libraries were sequenced using Illumina NextSeq500 sequencer to collect approximately 80k reads per cell.  Each cell was tagged with a Unique Molecular Identifier (UMI) sequence, which represents the identity of each single cell throughput the analysis pipeline.