WGS of mus musculus:RNaseH1 overexpression grup
After gDNA generation and deamination, we performed enzymic fragmentation and used DNA beads to selected the fragments at a size of 200bp-250bp. Next, we used Takara DNA SMARTTM ChIP-Seq Kit to construct strand specific cDNA library, following a procedure of T tailing, replication and tailing by RT, template switching and extension by RT, addition of adapters and amplification.