GSM5570861: h1;met1+/+ segregated from h1;met1+/- plant #3 (rna-seq rep2); Arabidopsis thaliana; RNA-Seq
We extracted total RNA from rosette leaves of 4-5-week-old plants using Trizol (Invitrogen, cat. No. 15596026) according to manufacturer's manual. To remove DNA from samples, 5 ug of RNA was treated with DNA-free DNA removal kit (Thermo, AM1907). 100-700 ng of DNA-free total RNA was subjected to RNA library construction according to manufacturer's protocol (Illumina, 20020640 and 20019792).