GSM6261089: HES-1, Conventional, rep0; Homo sapiens; RNA-Seq
hPSCs were detached from the matrix or MEFs by treatment with Collagenase type IV. Total RNA was extracted using the RNeasy Mini Kit (QIAGEN). mRNA libraries were prepared from 1 µg RNA using the KAPA Stranded mRNA-Seq Kit, with mRNA Capture Beads, following manufacturer's protocols .