VDR-USP6
RNA-based next-generation sequencing (SarcomaFusion Assay) was performed at the Massachusetts General Hospital. RNA was extracted from formalin-fixed paraffin-embedded tissue using Agencourt FormaPure Kit (Beckman Coulter, Indianapolis, IN, USA). Anchored multiplex PCR was performed using custom-designed ArcherDx primers (ArcherDx, Boulder, CO, USA). Sequencing was performed using the Illumina MiSeq, and sequences were aligned to the hg19 human genome reference and annotated using laboratory-developed algorithms in conjunction with the Integrated Genome Viewer (Broad Institute, Cambridge, MA, USA).