GSM5742419: mCtx_Cyto_rep 2_Small; Mus musculus; ncRNA-Seq
Total RNA was collected with the Trizol LE regent for cytoplasmic RNAs, and Trizol reagent for soluble nucleoplasmic and chromatin-associated RNAs. Short RNA below 200 nt was selected using RNeasy MinElute Cleanup Kit (Qiagen). RNA was processed with Ribo-minus kit to remove ribosomal RNA. Libraries were constructed with the Illumina TruSeq Small RNA sample Prep Kit. A final library of 120-160 bp was size selection with an TBE gel. The libraries were subjected to 100 bp single-end sequencing (MiSeq).