GSM5287160: F60Y; Coturnix japonica; RNA-Seq
An aliquot of blood was placed in Qiazol lysis reagent and homogeneized; Total RNA was extracted from the aqueous layer formed after chloroform precipitation, using the RNA Clean & Concentrator Kit-5 (Zymo Research, item # R1013) following manufacturer's instructions and including the DNase I in-column treatment step. RNA libraries were prepared for sequencing using NEBNext® UltraTM RNA Library Prep Kit, following standard protocols