small RNA sequencing of spinach: mature female flower
After total RNA was extracted by TRIzol, the RNA molecules in a size range of 1830nt were enriched by polyacrylamide gel electrophoresis(PAGE). Then the 3 adapters were added and the 36-44nt RNAs were enriched. The 5 adapters were then ligated to the RNAs as well. The ligation products were reverse transcripted by PCR amplification and the 140-160bp size PCR products were enriched to generate a cDNA library and sequenced using Illumina HiSeq TM  2510.