GSM5064499: 092_D12_63_033_POST_A; Homo sapiens; RNA-Seq
Cells were directly sorted into plates containing 2ul lysis buffer. Perform reverse transcription and PCR pre-amplification. PCR pre-amplified product was purified twice, using 0.8x Ampure-XP beads. 0.3 to 0.5 ng of pre-amplified cDNA was used to generate barcoded Illumina sequencing libraries (Nextera XT library preparation kit, Illumina) in 8µL reaction volume SMART-Seq2; Libraries were pooled and sequenced on the HiSeq2500 Illumina platform to obtain 50-bp single end reads