GSM5831551: HC_9; Homo sapiens; RNA-Seq
PBMC were isolated from whole blood. Lineage (CD3, CD4, CD14, CD16, CD19, and FceR1a)-CD45+CD161+CRTH2+CD127+ cells were identified as ILC2s and were sorted using a FACSAria IIIu flow cytometer (BD Bioscience). Library construction were performed using the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (New England Biolabs Japan), according to the manufacturer's instructions.