Genome sequencing of Erythrobacter sp. SDW2
To generate libraries, 5 g of genomic DNA was sheared using the Megaruptor3 G-tube according to the manufacturers recommended protocol, and then purified to remove small fragments by AMpureXP bead purification. The SMRTbell library was constructed by using SMRTbell Express Template Preparation Kit (101-357-000). The genome size, library size, and Qubit concentration were entered into calculator provided by PacBio, and the library was pooled according to the numerical value. DNA below 3 kb was removed using AMpure XP bead. The sequencing primer v4 was annealed to the SMRTbell template, and DNA polymerase was bound to the complex (Sequel Binding kit 3.0). The complex was purified by AMPure Purification to remove excess primer and polymerase prior to sequencing. The SMRTbell library was sequenced using Sequel Sequencing Kit v3.0 and SMRT Cell 1M v2, and the data was collected through 1  10 hour movie per each SMRT Cell 1M v2 using the sequel (Pacific Biosciences) sequencing platform.