GSM4848228: Exp3_totalRNA_Pol2_siControl_Input; Homo sapiens; RNA-Seq
[Table S6] RNA was purified using Nucleozol reagent (Macherey Nagel). [Table S6] Fragmentation, 3' adapter ligation, cDNA synthesis, second adapter ligation and enrichment [Table S2; Table S7] RNA was purified using Nucleozol reagent (Macherey Nagel). [Table S2; Table S7] Fragmentation, targeted cDNA synthesis, second adapter ligation and enrichment [Table S7] RNA was purified using Nucleozol reagent (Macherey Nagel). [Table S7] Column size selection (under 200nt), fragmentation, 3' adapter ligation, cDNA synthesis, second adapter ligation and enrichment [Table S4; Table S5] RNA was purified using Nucleozol reagent (Macherey Nagel). [Table S4; Table S5] Fragmentation, 3' adapter ligation, cDNA synthesis, second adapter ligation and enrichment [Table S3] in vitro transcribed RNA [Table S3] cDNA synthesis, PCR enrichment [Figure 5b] RNA was purified using Nucleozol reagent (Macherey Nagel) and treated with Ribozero (Illumina). [Figure 5b] Fragmentation, 3' adapter ligation, cDNA synthesis, second adapter ligation and enrichment