GSM6304064: CD8 High_5; Homo sapiens; RNA-Seq
mRNA isolation was immediately performed after sorting of CD8low and CD8high T cells using the RNeasy micro kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. 14ng of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using QuantSeq 3'mRNA-Seq Library Prep Kit-FWD and UDI 12 nt Unique Dual Indexing Add-on kit (both Lexogen, Vienna, Austria) according to manufacturer's instructions.