GSM6260039: PBMC; Mus musculus; RNA-Seq
0.5 ml of cardiac blood was collected from naive mice and lysed in Ammonium-Chloride-Potassium (ACK) buffer. The Chromium Next GEM Single Cell 3' Reagent kit v3.1 (Dual Index) was used. Barcoding and library preparation was performed following the manufacturer's protocols. Briefly, to generate single-cell gel-bead-in-emulsion (GEMs) solution, approximately 15,000 cells, in a final volume of 43 µl, were loaded on a Next GEM Chip G (10x Genomics) and processed with the 10x Genomics Chromium Controller