Viral targeted-capture of SARS-CoV-2: COVID-19 patient
Nucleic acid purification (KingFisher Duo Prime Purification System; KingFisher Duo Prime Purification System), reverse transcription (DNA using Maxima H Minus Double-Stranded cDNA Synthesis Kit), library preparation (Nextera Flex for Enrichment with RNA Probes), Tagmentation (Enrichment Bead-Linked Transposomes), Tagmented DNA cleanup (AMPure XP magnetic beads), Quantification (Qubit dsDNA BR Assay Kit), sequencing (Illumina Miseq; Reagent Kit v3 with 150 cycles)