GSM6261409: H3K9me3_HMLE-TWIST_DMSO_rep2; Homo sapiens; ChIP-Seq
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Sequencing libraries were made from 5ng of ChIP DNA using the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB) or 1ng of ChIP DNA using Nextera XT DNA Library Prep kit (Illumina).