GSM5618460: TCR NKG2A+ CD8+ T cell 03; Homo sapiens; RNA-Seq
Naïve, memory, NKG2A+, and KIR+ CD8+ T cells were isolated using a FACSAria II cell sorter (BD Biosciences). RNA-seq libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit (Lexogen) and sequenced on an Illumina NextSeq 500 for RNA sequencing. The TCR library was prepared using the SMARTer® Human TCR a/b Profiling Kit (Takara Bio, USA) and sequenced on an Illumina MiSeq