RNAseq for GFP_glmS episomal overexpression parasites
For strand specific RNA sequencing, parasites carrying the control GFP-glmS episomal construct and PfHDAC1-GFP-glmS episomal construct were cultured in triplicate at 8% parasitemia and 2% hematocrit. Post double synchronisation the cultures were harvested at roughly 24-hour post invasion time point. Post harvesting the parasites we proceeded with isolation of RNA from the TriZol suspended samples. The Thermo Colibri 3 mRNA library preparation kit was utilised for preparation of strand specific libraries. The final amplified libraries were quality checked on Agilent bioanalyzer for size distribution and Qubit fluorometer for quantity. Single end sequencing was done on the Illumina NextSeq 550 platform (150bp read length).