WGS of Homo sapiens: Sample F21-001_WGS
PCR-free libraries were generated from 1 microgram genomic DNA. A Covaris LE220-plus was used to shear the DNA and the TruSeq DNA PCR-Free HT Sample Preparation Kit (Illumina) was used for library generation. The median insert sizes were approximately 400 bp. Libraries were tagged with unique dual index DNA barcodes to allow pooling of libraries and minimize the impact of barcode hopping. Libraries were pooled for sequencing on the NovaSeq 6000 (Illumina) using version 1 chemistry to obtain at least 320 million 151-base read pairs per library. Data was processed using RTA ver. 3.4.4.