7-male blood
Each DNA sample is quantied by agarose gel electrophoresis and Nanodrop (Thermo). Libraries were prepared using Illumina standard protocol. Briefly, 3 microgram of genomic DNA was fragmented by nebulization, the fragmented DNA is repaired, an A is ligated to the 3 end, Illumina adapters are then ligated to the fragments, and the sample is size selected aiming for a 350400 base pair product. The size selected product is PCR amplified (Each sample is tagged with a unique index during this procedure), and the final product is validated using the Agilent Bioanalyzer.