GSM5285749: PBMCs from healthy controlHIP043; Homo sapiens; ATAC-seq
The single-cell ATAC sequencing was performed by using the Chromium Next GEM Single Cell ATAC Reagent Kits v1.1 (10x Genomics, PN-1000175) and following the Demonstrated Protocol (Nuclei Isolation for Single Cell ATAC Sequencing) provided by the 10x Genomics company. Briefly, cryopreserved PBMCs were thawed and 50,000-500,000 cells were aliquoted, washed with PBS and then lysed with 100 Œºl lysis buffer for 4 min. The lysed nuclei were centrifuged after washing with 1 ml washing buffer, and resuspended in Diluted Nuclei buffer. The nuclei concentration was then determined with TC20‚Ñ¢ Automated Cell Counter (Bio-rad, #1450102), and approximately 6,000 nuclei were used for Tn5 transposition, single nuclei barcoding and library preparation following the instructions in the kit. Library preparation was performed with the Chromium Next GEM Single Cell ATAC Reagent Kits v1.1 (10x Genomics, PN-1000175), according to the manufacturer protocol