GSM6216235: H9 hESC, undifferentiated, Day 0, rep. 2; Homo sapiens; RNA-Seq
Total RNA was extracted with TRIzol Reagent (Invitrogen) following the manufacturer's guidelines. Total RNA was depleted for ribosomal RNA (rRNA) using the Ribo-Zero rRNA Removal Kit (Human, Mouse, Rat) (Epi-centre, WI, USA), and the sequencing libraries generated using the ScriptSeq v2 RNA-Seq Library Preparation Kit (Epicentre). The libraries were quality controlled on the 2100 Bioanalyzer (Agilent Technologies) and quantified using the KAPA library quantification kit (Kapa Biosystems, MA, USA). RNA-Seq was performed on the HiSeq 4000 system (Illumina, San Diego, CA, USA) using the 100 bp paired-end sequencing protocol.