GSM6216323: AALE, pBABE control, ATAC; Homo sapiens; ATAC-seq
100,000 AALE cells were collected and centrifuged at 500xg for 5 minutes at 4C. Pellets were washed with ice-cold PBS and centrifuged. Pellets were resuspended in ice-cold lysis buffer. Tagmentation reaction and purification were conducted according to manufacturer's protocol (Active Motif) Active Motif ATAC-seq kit, 2x75bp PE reads