GSM5144962: 7208-2_KitSP_MPN; Mus musculus; miRNA-Seq
c-Kit+ splenocytes were harvested from three independent mice per each genotype Dnmt3afl/fl,Flt3ITD/ITD,Mx1Cre (AML) and Flt3ITD/ITD (control). Red blood cells were lysed, and RNA was extracted by TriZol according to Meyer et al., 2009. RNA quality and quantity were measured by Agilant Bioanalyzer. Then samples with a RIN>8.0 were processed. A size range of 120 bp to 165 bp was isolated to select small RNA libraries on the Pippin Prep (Sage). Size selected libraries were each sequenced at 20 million reads. small RNAs were detected by RNAseq analysis using single-read 75 nucleotide (nt) sequencing and 20 million reads for all samples. RNA Seq libraries were generated with Pippin Prep kits and size selected for small RNA fragements.