Illumina HiSeq 2500 paired end sequencing; Transcriptome comparison of active and regressed testes of Microtus duodecimcostatus
Transcriptome comparison of active and regressed testes of Microtus duodecimcostatus
Three reproductively active (winter) and three reproductively inactive (summer) Mediterranean pine vole adult males (Microtus duodecimcostatus) were captured alive in isolated small wastelands populations near the localities of Las Gabias and La Malaha (Granada province, south-eastern Spain), with the permission of the Andalusian environmental authorities (ConsejerÃ­a de Agricultura, Pesca y Medio Ambiente) and handled following the guidelines and approval of both the Ethical Committee for Animal Experimentation of the University of Granada and the Andalusian Council of Agriculture and Fisheries and Rural development (Registration number: 450-19131; June 16 th , 2014). total RNA samples were extracted from whole testis with the Quiagen RNeasy Mini Kit, treated with DNaseI and cleaned with Quagen RNeasy minikit clean up. After successfully passing quality check,libraries were constructed for an Illumina HiSeq 2500 System, according to the manufacturer instructions.