GSM4851077: mCtx_Nuc_rep 1_Total; Mus musculus; RNA-Seq
Total RNA was collected with the Trizol LE regent for cytoplasmic RNAs, and Trizol reagent for soluble nucleoplasmic and chromatin-associated RNAs. Long RNA over 200 nt was selected using RNeasy MinElute Cleanup Kit (Qiagen). RNA was processed with Ribo-minus kit to remove ribosomal RNA. Libraries were constructed with the Illumina TruSeq stranded kit by skipping oligo-dT bead incubation step. A final library of 250-400 bp was size selection with an agarose gel.