GSM5770085: Control_Cortex_c55 [RNA-seq]; Rattus norvegicus; RNA-Seq
RNA was extracted rom the perilesional cortex and ipsilateral thalamus using a mirVana miRNA isolation kit (#AM1560, Life Technologies (Ambion) Carlsbad, CA, USA), QIAshredder (#79654, Qiagen), and AllPrep DNA/RNA Mini Kit (#80204, Qiagen). Thereafter, RNA purification was performed using miRCURY™ RNA Isolation Kit (#300111, Exiqon, Vedbaek, Denmark). The library preparation was done using TruSeq® Stranded mRNA Sample preparation kit (Illumina inc). The starting material (300 ng) of total RNA was mRNA enriched using the oligodT bead system. RNA-Seq. Nextseq500 instrument using High Output sequencing kit (75 cycles) according to the manufacturer instructions (Illumina Inc.).