GSM5285795: SB/AKT/NRas + saline 4; Mus musculus; RNA-Seq
Liver tissue was snap-frozen in liquid nitrogen; RNA extracted using RNAzol reagent. Library was prepared as described in Grubman et al. (2021). Briefly, index was added during initial pA priming and pooled samples amplified using template switching oligo. P5 is added by PCR and P7 by Nextera transposase. Sequencing was performed according to Illumina protocols using NextSeq 550 v2.5 kit.