GSM5570304: ATAC-seq_CD4rest_0h; Homo sapiens; ATAC-seq
Nuclei were isolated with Transposition buffer (30 mM Tris acetate Buffer, 60 mM Potassium acetate solution, 10 mM Magnesium acetate solution, 0.25% NP-40 solution, 16% DMF and 1X Proteinase Inhibitors). Isolated nuclei were subjected to tagmentation with Tn5 transposase and library construction with the Illumina Tagment DNA TDE1 Enzyme and Buffer Kit (Illumina)