Illumina sequencing of Arima Hi-C library of Pectinophora gossypiella: female 4th instar larva
Tissue from a single whole insect (ilPecGoss2) was crosslinked using the Arima Hi-C low input protocol, and proximity ligation was performed using the Arimas Hi-C Kit. After proximity ligation, the DNA was sheared using a Diagenode Bioruptor and then size-selected to enrich for DNA fragments of 200-600bp. An Illumina library was prepared from the sheared and size-selected DNA using the Swift Accel NGS 2S Plus kit. The final Illumina Hi-C library was sequenced on a NovaSeq 6000 System.