GSM5618393: Vehicle 1; Mus musculus; RNA-Seq
Animals were euthanized,  perfused and their brains isolated. Brain tissue was enzymatically digested with Accutase and filtered through a 70micron filter. Microglia were enriched by percoll gradient centrifugation then magnetically isolated using a CD11b positive selection kit. RNA was extracted using a GeneJet RNA purification kit and then SMART-Seq v4 PLUS kit was used to generate high-quality cDNA libraries. high-quality Illumina sequencing-ready libraries were prepared and sequencing was performed using with Illumina Hi-seq lane which generated an average of 47.9 million 150nt paired-end reads per sample with a minimum FastQC score of 34.