GSM5832783: HPL mBG _p4/p5_1_880; Homo sapiens; RNA-Seq
RNA was isolated using Macherey&Nagel RNA isolation kit according to the manufacturers protocol. The quality of total RNA isolates was analyzed using the Agilent RNA 6000 Nano Kit.   Poly-(A)-selection was performed utilizing the NEBNext Poly(A)mRNA Magnetic Isolation Module (NEB) according to the manufacturer's requirements. mRNA libraries were prepared with the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB). All libraries were analyzed with the Agilent DNA 1000 Kit and quantified using the Qubit® dsDNA BR Assay Kit (ThermoFisher). After equimolar pooling all samples were sequenced on an Illumina HiSeq 1500 system with High Output chemistry v4 (50 cycles, single-read).