GSM5618333: CB-SA-3; Homo sapiens; RNA-Seq
Total RNA was isolated from maternal blood and cord blood collected into PAXgene blood RNA tubes using the PAXgene Blood RNA Kit (Qiagen). The purity, concentration, and integrity of the RNA samples were assessed using the NanoDrop 1000 spectrophotometer and the Bioanalyzer 2100. The RNA-seq library was prepared by BGI Genomics (ShenZhen, China) using the Oligo dT Stranded mRNA library preparation protocol (BGI, Hong Kong). Paired-end sequence reads (at least 50 million paired-end reads) of 150 bp length were generated using DNBseq (MGI-G400), and the raw data were provided by BGI.