GSM6303836: OPA1_Control_2; Mus musculus; RNA-Seq
RNA was extracted using RNeasy Kit (QIAGEN) following the manufacturer's instructions with an additional on-column DNase treatment using the RNAse-Free DNase set (Qiagen). RNA was quantified using Qubit 2.0 (Thermo Fisher Scientific). Libraries were prepared using the TruSeq stranded mRNA kit (Illumina) and sequenced in a HISeq 3000 (Illumina) with a read depth of approximately 30 million aligned reads per sample by the Deep-sequencing Facility at the Max-Planck-Institute for Immunobiology and Epigenetics (Freiburg)