GSM5993270: DEN-PT-WD rep 7; Mus musculus; RNA-Seq
Total tissue was pulverized with mortar and pestle under a liquid nitrogen atmosphere and RNA was extracted from the equivalent of 20-30mg frozen tissue in TRIzol Reagent (Thermo Fisher Scientific, UK) followed by phenol removal with chloroform. RNA was further purified with the RNeasy Mini Kit (Qiagen, UK). DNase treatment was performed to avoid genomic DNA contamination. RNA quantification and quality controls for integrity and purity (Nanodrop, Qubit and Agilent 2100 Bioanalyzer). Libraries were prepared using KAPA mRNA HyperPrep Kit (Kapa Biosystems)