GSM5287237: ApcMin2 intestinal adenoma; Mus musculus; RNA-Seq
For tumor dissociation, 3-10 tumors of approximately same size and from similar locations in the intestines were removed from 11-week-old ApcMin and VApcMinL mice. Tumors were incubated with enzymes (4 mg/ml Dispase II, 1 mg/ml Collagenase I, 1 mg/ml Collagenase H and 1 U/ml DNase I) for 30 min at +32°C, followed by incubation with trypsin-EDTA for 15 min at +37°C. Cells were filtered through 40 μm mesh and subjected to scRNA-seq without FACS sorting. All samples were analyzed with the Chromium Single-Cell 3'RNA-sequencing platform (10x Genomics, Pleasanton, CA) with the Reagent Kit V2 (ApcMin and VApcMinL) or V3 (ApcMin2 and VApcMinL2) according to the manufacturer's instructions. Sample libraries were sequenced using the Illumina NovaSeq 6000 system (50,000 reads per sample).