GSM5333085: UUO #1 10 days; Mus musculus; RNA-Seq
Kidneys were collected and subjected to single cell disregation protocol as described in Youssef et al. 2021. Single cell preprartion from whole kidney were processed to generate cDNA libraries according to Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 protocol (10X Genomics). Pooled libraries were then loaded on a HiSeq2500 instrument (Illumina) and sequenced to obtain 75 bp paired-end reads. scRNA-seq (single-cell RNA-seq)