GSM5287359: 58_VAL-RA-058; Homo sapiens; RNA-Seq
Total RNA was isolated using a TissueRuptor and RNeasy Fibrous Tissue Mini Kit (QIAGEN) with an on-column DNase digestion (RNase-Free DNase Set, QIAGEN) to avoid genomic DNA contamination. Integrity of RNA samples was verified using an Agilent Bioanalyser with the RNA 6000 Nano labchip® kit (Agilent). Concentration and purity were determined with a Nanodrop ND-1000 spectrophotometer. Libraries were constructed using the Truseq stranded mRNA sample prep kit (Illumina, San Diego, CA,USA) according to the manufacturer instructions and sequenced on two lanes on an Illumina NovaSeq 6000 (single-end, 100bp, flow cell S2).