GSM4979876: ATAC-seq_shHNF1B-1_Rep1; Homo sapiens; ATAC-seq
Cells at 75% confluency were trypsinised, counted and 50,000 cells were subjected to ATAC-seq protocol directly as previously described (Buenrostro et al., 2013). Libraries were prepared using the Illumina Nextera DNA library preparation kit (FC-121-1030) and purified using the minElute PCR purification kit (Qiagen 28004). Following purification, library fragments were amplified using NEBNext PCR master mix and custom Nextera PCR primers. Libraries were amplified for a total of 8-12 cycles and cleaned-up using the Agencourt AMPureXP reagent (A63880) according to manufacturer's recommendations, and pooled in equimolar concentrations prior to sequencing.