GSM5285290: T6122; Mus musculus; RNA-Seq
RNA was extracted from tumor tissue using RNeasy mini kit (QIAGEN, Germantown, MD). The integrity of RNA was assessed using Agilent Bioanalyzer and samples with RIN >5.0 were used. Libraries were constructed using NEBNext® Ultra™ RNA Library Prep Kits (non-directional) for Illumina, following manufacturer protocols. mRNA was enriched using oligo-dT beads.