GSM6044881: ARcKO_DHT_rep3; Mus musculus; RNA-Seq
Th2 cells were lysed with Buffer LTR (Qiagen), and RNA was purified with RNeasy Micro Kit (QIAGEN). Double strand cDNA was synthesized using SMART-Seq HT kit for Sequencing (Takara), and sequencing libraries were constructed with Nextera XT DNA Library Preparation Kit (Illumina) according to the manufacturer's instructions. RNA libraries were prepared for sequencing using standard Illumina protocols