GSM5451954: CTL3; Mus musculus; RNA-Seq
Total RNA was extracted from prefrontal cortex (PFC) of male mice at 9 weeks with the AllPrep DNA/RNA Mini Kit (#80204, Qiagen, Hilden, Germany) according to the manufacturer's instruction. RNA integrity number (RIN) of total RNA was quantified by Agilent 2100 Bioanalyzer using Agilent RNA 6000 Pico Kit (#5067-1513, Agilent, Santa Clara, CA). RNA-seq was performed as a service by Bioengineering Lab. Co., Ltd. (Kanagawa, Japan). Briefly, total RNA with RIN values of ≥8.8 were used for RNA-seq library preparation. mRNA was purified from 500 ng total RNA, and subjected to cDNA library making (fragmentation, first and second strand syntheses, adenylation, ligation and amplification) by TruSeq Stranded mRNA Library Prep (#20020594, Illumina, San Diego, CA) according to the manufacturer's instruction. cDNA library quality was quantified by 2100 Bioanalyzer using Agilent High Sensitivity DNA Kit (#5067-4626, Agilent, Santa Clara, CA). Library was sequenced as 151 bp paired-end on Illumina NextSeq 500.