GSM6261317: CRISPR_CTRL_KAN1_C; Homo sapiens; RNA-Seq
Total RNA was isolated using the RNeasy kit following the manufactures protocol. Quality and integrity of total RNA was controlled on Agilent Technologies 2100, Bioanalyzer (Agilent Technologies; Waldbronn, Germany). The RNA sequencing library was generated from 100 ng total RNA using NEBNext® Single Cell/Low Input RNA Library to manufacture´s protocols.