GSM6044804: Embryonic Fibroblasts 4h UT rep3; Myotis lucifugus; RNA-Seq
RNA was harvested using Zymo Quick-RNA Miniprep Plus Kit (Zymo #R1057). All lysates were frozen at -80°C prior to extraction, and all extracted RNA were stored at -80°C as single-use aliquots. Libraries were generated with 200ng or 500ng RNA using KAPA mRNA HyperPrep Kit (KAPA #KK8581) according to the manufacturer's instructions. Briefly, single-indexed adapters (KAPA #KK8700) or unique dual-indexed adapters (KAPA #KK8727) were added to each sample at a final concentration of 78 nM. Excess adapter was removed using sequential 0.63X and 0.70X KAPA Pure Bead (KAPA #KK8002) cleanups. Purified, adapter-ligated samples were amplified for 10 (500ng input RNA) or 12 (200ng input RNA) cycles according to the manufacturer's instructions and purified using a 1.0X KAPA Pure Bead cleanup.