GSM5742334: HD-mNDN_5; Homo sapiens; RNA-Seq
Total RNA was extracted after cell lysis by the RNeasy Mini Kit (Qiagen, Venlo, Limburg, Netherlands). To completely remove any possible contaminating DNA, an on-column DNase digestion with the RNase-free DNase set (Qiagen) was performed during total RNA isolation. Libraries for transcriptome analysis were prepared using Smart-seq2 protocol (PMID: 24056875)