Illumina HiSeq 4000 paired end sequencing; Blood RNA sequencing was performed on a cohort of adults attending the Emergency Department with suspected infection who had subsequently-confirmed viral, bacterial, or no infection diagnoses
Blood RNA sequencing was performed on a cohort of adults attending the Emergency Department with suspected infection who had subsequently-confirmed viral, bacterial, or no infection diagnoses
Whole blood was collected at the time of recruitment in Tempus Blood RNA tubes and total RNA was isolated with the TempusTM Spin RNA Isolation Kit (ThermoFisher Scientific) according to the manufacturer's instructions.  RNA samples were stored at −80 °C until further analysis. After additional DNAse treatment, library preparation at paired end reads was conducted using the Illumina's TruSeq® RNA Sample Preparation Kit; ribosomal and globin RNA depletion