Millettia speciosa leaf sample
The leaf samples of M. speciosa were collected in the Guangdong Academy of Forestry, Guangdong province of China (23 11 59 N, 11322 7 E). And the total genomic DNA of M. speciosa was extracted from these samples with the modified CTAB method The extracted high-quality DNA was used to construct a library, using NEBNext Ultra DNA Library Prep Kit for Illumina. After passing the quality test, the library was sequenced using the Illumina NovaSeq system. We utilized Flye (v2.8.3) software to assemble the chloroplast genome and performed BLAST (v2.2.30) alignment to obtain the candidate sequences. Then we applyed Gapcloser (v1.12) to repair the chloroplast candidate sequences containing gap (including N sequence) to get the complete chloroplast genome of M. speciosa. Finally, the chloroplast genome was annotated by CPGAVAS2 software.