16S of Podocnemis unifilis: cloaca day25
WeextractedbacterialgenomicDNAfromswabsamplesfollowingstandardprotocolsfortheNucleoSpin96Soilextractionkit(Macherey-Nagel,Germany).Weperformedatwo-stepPCRwiththeprimers515Fand806Rtosequencea291-bpfragmentofthehypervariableV4regionofbacterial16SrRNAandfollowedtheFluidigm(AccesArrayBarcodeLibraryforIlluminaSequencers-384,SingleDirection,FluidigmCorporation,SouthSanFrancisco,CA,USA)protocolforsequencingontheIlluminaMiSeqsequencingplatform