GSM5737569: CORT-Sal (#9969); Mus musculus; RNA-Seq
Brains were harvested, and the prefrontal cortices were rapidly dissected and transferred into ice-cold Hibernate A/B27 medium (60 ml Hibernate A medium with 1ml B27 and 0.15 ml Glutamax). The tissues were enzymatically dissociated into single cell suspension, and the cells were captured with the 10X Chromium platform (10X Genomics, Pleasanton, CA). Reverse transcription, cDNA amplification and library construction were performed according to the manufacturer's instructions and used for sequencing.