GSM5065520: NANOS2 KO 49 dpi rep 1; Mus musculus; RNA-Seq
Sorted germ cells were frozen in Trizol and total RNA was recovered using Trizol manufacturer protocol.  Whole testes were snap-frozen on dry ice then recovered using Trizol manufacturer protocol.  polyA mRNA was enriched using NEBNext poly(A) mRNA Magnetic Isolation Module (E7490S, New England Biolabs) according to the manufacturer's instructions. 10 ng of mRNA was used to make barcoded libraries with the Ion Total RNA-seq Kit V2 (Life Technologies).  Libraries were pooled to 75 pM before loading on Ion P1 semiconductorr chips using and Ion Chef and sequenced on and Ion Proton sequencer.