NextSeq 550 paired end sequencing; Biosynthetic Gene Cluster regulatory sequence library profiled in S. albidoflavus
Biosynthetic Gene Cluster regulatory sequence library profiled in S. albidoflavus
Samples were harvested during mid-log growth phase Nucleic acid samples were prepped using beadbeating followed by phenol-chloroform extraction and ethanol precipitation DNA samples were prepped through a two step PCR method to enrich for amplicons of interest. RNA samples were prepped by gene-specific RT and adapter ligation. Subsequently, two step qPCR was performed to add select for sequences of interest and append Illumina adapter sequences