GSM6044818: HEK293_TNRC18_KI_MUT_TRIM28; Homo sapiens; OTHER
1 million of cells was collected, washed and bound to ConA beads. Cell:bead sample was incubated with indicated antibodies overnight and then washed and treated with pAG-MNase, followed by adding 2mM of CaCl2 to activate pAG-MNase. Solubilized chromatin was then released using the CUT&RUN stop buffer. DNA purification was carried out with the PCR cleanup kit (NEB, Monarch PCR & DNA Cleanup Kit, #T1030). Libraries were prepared by NEBNext Ultra II kit (NEB, #E7645L) with 20ng CUT&RUN DNA as input.