GSM6044921: TU_pos1; Mus musculus; RNA-Seq
Tumor-free omenta or omental tumors were excised, and ST2+ and ST2- Tregs were collected directly into Qiagen RLT buffer plus 2-mercaptoethanol (Sigma). Total RNA was purified using the Quick-RNA MicroPrep kit. Total RNA was used as input for the SMART-seq v4 cDNA synthesis kit (Takara). The resulting cDNA was then used as input for the NexteraXT kit (Illumina) for tagmentation, PCR amplification and indexing. Final libraries were quantitated by Qubit, pooled at equimolar ratio.