GSM5144805: T_2; Homo sapiens; RNA-Seq
Total RNA was extracted from cells using TRIzolTM Reagent (#15596026, Invitrogen, Carlsbad, CA, USA) per the manufacturer's protocol, and ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Epicentre, Madison, WI, USA). Fragmented RNA (average length of ~200 bp) were subjected to first-strand and second-strand cDNA synthesis followed by adaptor ligation and enrichment with a low cycle per the instructions of the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA). The purified library products were evaluated using the Agilent 2200 TapeStation and Qubit 2.0 (Life Technologies, USA).