GSM5380283: 3-10; Canis lupus familiaris; ssRNA-seq
VAT was collected by laparotomy before and after ACTH treatment. Total RNA was isolated from VAT tissue with the RNeasy Lipid Tissue Mini Kit (Qiagen GmbH, Hilden, Germany). The libraries were prepared following Illumina TruSeq stranded mRNA protocol. The quality of the RNA and final libraries was determined using an Agilent 4200 TapeStation System. The libraries were pooled equimolecularly and sequenced in an Illumina NovaSeq sequencer (single-end 100 bp) with a depth of around 20 Mio reads per sample. Illumina TruSeq stranded mRNA protocol