GSM5531505: Cas9_HBG_vitro_0.5mM_IP; Homo sapiens; ChIP-Seq
Genomic DNA was freshly extracted using FastPure Cell/Tissue DNA Isolation MiniKit and stored in 25 mM K3BO3 buffer (pH 7.0) at -20 °C. Treated genomic DNA was  purified by DNeasy Blood & Tissue Kit and eluted by  25 mM K3BO3 buffer (pH 7.0). For library preparation, fragmented DNA first be repaired and ligated to adapters, then, the IP sample was subjected to biotin enrichment using Dynabeads MyOne Streptavidin C1 (Thermo, 65001). All reactions were followed the protocol of VAHTS Universal DNA Library Prep Kit for Illumina V3 (Vazyme, ND607) with some modifications. The biotin pull-down experiments were performed following the recommendations from the Dynabeads MyOne Streptavidin C1 (Thermo, 65001) with some modifications. CROss-seq (biotin enriched ChIP-seq), Amplicon