GSM5832763: DMD-CO_day56_3 [GC115822]; Homo sapiens; RNA-Seq
The organoids were harvested in lysis buffer and the total RNA was extracted for sequencing analysis at the GenomicsCore of KU Leuven using single-read sequencing technique on the HiSeq4000 platform (Illumina Inc.). RNA (>10 μg) extracted from DMD-CO and DMD-Iso-CO on day 56 were verified and processed by the Genomics Core (KU Leuven – UZ Leuven). As quality control, the RNA concentration was measured with Nanodrop and quality was checked with Bioanalyzer. The Lexogen QuantSeq 3' mRNA-Seq library prep kit was used according the manufacturer's protocol with 500 ng input. After the prep the libraries were measured with Qubit and put on the Fragment analyzer so the libraries can be pooled equimolar to 2 nM. The pool was then quantified with qPCR and a final pool (2 nM) was made for single-read sequencing on the HiSeq4000 (Illumina Inc). The settings were 51-8-8.