GSM5743390: ILC2_WT_RNA; Mus musculus; RNA-Seq
total RNA was isolated using the RNeasy Plus Micro Kit (Qiagen). RNA integrity numbers were determined using a TapeStation 2200 (Agilent), and all samples used for RNA-seq library preparation had RIN numbers greater than 9. Libraries were prepared using the SMARTer® Stranded Total RNA-seq Kit v2-Pico Input Mammalian kit (Takara). Two technical replicates were generated for each experiment. Libraries were validated for quality and size distribution using a TapeStation 2200 (Agilent). Libraries were paired-end sequenced (38bp+38bp) on a NextSeq 500 (Illumina) RNA libraries were prepared for sequencing using standard Illumina protocols