GSM4848620: Gro_seq_RAW_GPS2_KO_LPS_60m_rp2; Mus musculus; OTHER
WT and GPS2 KO RAW cells were treated with 100 ng/ml LPS (0, 30 min, 1 hour) and cell nuclei were extracted. The nuclear run-on reaction was performed for 7 minutes and RNA hydrolysis was allowed to proceed for 10 minutes. All GRO-seq library preparations were performed in parallel to avoid batch effects. ChIPed DNA was processed using Takara SMRTer ThruPLEX DNA-seq Kit.