GSM6044869: Ctrl MG-2; Mus musculus; RNA-Seq
Total RNA was isolated using the Trizol reagents from control mammary glands or a central piece of the tumor tissue. The total RNA was treated with DNase I and further cleaned using the Qiagen RNeasy clean up kit and RNeasy micro kit, as per the manufacturer's protocols. Illumina TruSeq RNA Sample Prep Kit v2 (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries following the manufacturer's protocols and sequenced using the 2 x 75 bases paired-end protocol on a HiSeq 2000 instrument (Illumina). RNA libraries were prepared for sequencing using standard Illumina protocols.