RNA-seq Batrachuperus tibetanus
The sequencing libraries were constructed using the an NEBNext Multiplex Small RNA Library Prep Kit for Illumina (NEB New England Biolabs, Ipswich, USA) according to the manufacturers instructions. Briefly, first of all, ligated the 3 SR and 5 SR Adaptor. Then, reverse transcription synthetic first chain. Last, PCR amplification and Size Selection. PAGE gel was used to electrophoresis fragment screening purposes, rubber cutting recycling as the pieces get small RNA libraries. At last, PCR products were purified (AMPure XP system) and sequenced using the Illumina HiSeq TM 4000 system by LC Sciences (Hangzhou, Zhejiang, China)