GSM5832704: WT_hESC_rep2; Homo sapiens; RNA-Seq
Cells were removed from the plate with Accutase. For RNA extraction, cells were lysed in Trizol (Invitrogen), the aqueous layer was isolated as indicated by the manufacturer, and RNA was purified with RNeasy columns (Qiagen). DNase (Qiagen) treatment was added to the column prior to elution as per manufacturer instructions. Libraries for RNA-seq were prepared using the KAPA kit for PolyA enriched mRNA-seq (Illumina) according to the manufacturer's protocol.