GSM4849724: Untreated; Streptococcus pneumoniae; RNA-Seq
RNA was extracted using Qiagen RNeasy kit. DNA was depleted using Qiagen RNase-free Dnase. RNA integrity was determined using an Agilent Bioanalyzer. Performed by Australian Genome Research Facility. RNA was prepared using standard Illuminia Sequening protocols with Ultra Directional RNA kit (New England Biolabs)  to generate barcoded libraries.