GSM5811705: MDA-MB-231 siSTAT3+Vector Rep3; Homo sapiens; RNA-Seq
Total RNA was extracted by TRIzol reagent (Invitrogen). For RNA-Seq, libraries were prepared for sequencing using standard Illumina protocols. For Cut&Tag, 50,000 cells were harvested and the CUT&Tag assay was performed using a commercial kit (Vazyme, China, TD901-TD902) according to the manufacturer's protocol.