GSM5333105: AS5-1; Mus musculus; RNA-Seq
tumor cells were dissociated enzymatically by papain for 30 mins and then harvested by centrifugation. Single cells were partitioned into Gel Beads in Emulsion (GEMs) in the Chromium Controller instrument with cell lysis and barcoded reverse transcription of RNA, followed by cDNA amplification, shearing, library construction and indexing by PCR amplification. Subsequently, libraries were sequenced by Illumina 2x150 bp sequencing.