GSM4848923: Soleus HRT CTRL rep3; Rattus norvegicus; RNA-Seq
Plantaris muscle (50-80mg) were homogenized in QIAzol with steel beads in a Bullet Blender. Total RNA was harvested using the miRNeasy minikit. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. The same method was performed separately for soleus muscle. RNA libraries were prepared for sequencing using standard Illumina protocols