GSM5448688: 1089-B6C3-M39-P14-M-CB; Mus musculus; RNA-Seq
CB tissues were collected from P14 B6C3 WT and Wbscr17-/-, and RNA was purified using Trizol followed by 45 min of RNase-free DNase I treatment (NEB) and RNA Clean & Concentrator Kit (Zymo Research). The quality of RNA was tested by Agilent BioAnalyzer and Qubit RNA-seq libraries were generated using Illumina TruSeq RNA Library Prep Kits. Samples were sequenced as 150 bp paired end reads (≥ 23 million read pairs per sample) on Illumina Hi-Seq 4000 sequencer through the GENEWIZ sequencing service