PacBio transcript analysis of Drosophila simulans Sz12 male head tissue under ethanol treatment.
Flies were flash frozen and heads isolated on dry ice. Approximately 100 fly heads underwent total RNA isolation followed by full-length cDNA synthesis using the SMARTer PCR cDNA Synthesis kit. Single molecule SMRTbell libraries were constructed from each double-stranded cDNA sample using the IsoSeq protocol followed by PCR optimization and ELF size selection. Each library was sequenced at UF ICBR (Gainesville, FL).