GSM5380104: Ampulla2: Ampulla of healthy fallopian tube donor 2; Homo sapiens; RNA-Seq
Fresh human fallopian tube samples obtained from either surgical removal for benign indication and cadaver samples were used as tissue sources. The whole tissue sample was dissected into three anatomic segments (isthmus, ampulla, fimbria). These segments separately were used to create a single cell suspensions by mechanical dissociation and digestion with collagenase at 37C.  All live cells were selected by exclusion of DAPI positive cells by flow cytometry. 10x Chromium scRNA-seq; Single-cell suspensions were processed and single cell RNA-seq data were generated using the 10X Genomics Chromium platform.