GSM4979531: FoB Cic KO rep1; Mus musculus; RNA-Seq
B-1a (IgM+, CD19+, CD5+, CD43+) and Fo B (B220+, CD93-, CD23+, CD21lo-mid) cells were isolated from the spleen by FACS-sorting using a MoFlo-XDP (Beckman Coulter). Total RNA was extracted using TRIzol Reagent (GeneAll, Seoul, Republic of Korea) according to the manufacturer's protocol. DNA library for mRNA sequencing was prepared using a SMART-seq v4 Ultra Input RNA Kit (Clontech, for B-1a cells) and TruSeq RNA Sample Prep Kit v2 (Illumina, for Fo B cells), according to the manufacturer's protocols. Libraries were sequenced on the Illumina Hiseq 4000 sequencer.