GSM5617932: KO 4; Mus musculus; RNA-Seq
KPC tumors from livers of WT and HCK knock-out mice were removed and flash frozen on dry ice. RNA extraction on tumor samples was performed using the RN-easy Mini Plus kit (Qiagen). RNA was submitted to the Australian Genome Research Facility (AGRF) for sequencing on the Illumina NovaSeq 6000 platform with v1 200 cycle chemistry (100 bp paired-end). The Illumina TruSeq Stranded Total RNA Library Prep workflow with Ribo-Zero Gold was used to process the samples as per the manufacturer's instructions.