GSM5494802: HDAC4_pcDNA_subject2; Homo sapiens; RNA-Seq
Total RNA was isolated from the primary human dermal fibroblasts (HDFs) transfected with HDAC4 siRNA or HDAC4 overexpression vector and their controls (control siRNA and control pcDNA vector) using Trizol (Invitrogen, USA). Isolated RNA was fragmented using TruSeq Stranded Total RNA Sample Prep Kit (Illumina, San Diego, USA) according to the manufacturer's protocol, and libraries for sequencing were then generated based on reverse transcription of the fragmented RNA using Superscript II reverse transcriptase (Invitrogen, USA). After adaptor ligation, libraries were sequenced using HiSeq 2500 system.