GSM5737449: rdd D Rosette leaf BS-seq; Arabidopsis thaliana; Bisulfite-Seq
DNA was extracted using a CTAB protocol. BS-seq: Bisulfite conversion was performed on 200ng of DNA using an Invitrogen MethylCode bisulfite conversion kit. Libraries were prepared with a QIAGEN QiaSeq Methyl Library Kit following manufacturer's instructions. 6-11 cycles were used for amplification.  EM-seq: 100ng of DNA in TE buffer was sheared to an average 200bp fragment size using a Covaris S220. Following fragmentation, a 2.5x bead cleanup was performed with a final elution in water. Conversion and library preparation was performed using an NEBNext Enzymatic Methyl-Seq Kit (including spike-in controls) according to manufacturer's instructions with 6 cycles of amplification.