GSM5494859: WT LMS_RNA_rep1; Arabidopsis thaliana; RNA-Seq
Total RNA of late microspore (about 100000 microspores per sample) was extracted using an RNeasy Plant Mini Kit (QIAGEN). Subsequent cDNA synthesis and amplification were performed using a SMART-Seq® HT Kit (Takara), and then purified using an Agencourt AMPure Purification Kit (Beckman Coulter). Purified cDNA was quantified by a Qubit 3.0 Fluorometer (Life Technologies) and validated using a Bioanalyzer 2100 system (Agilent Technologies). Sequencing libraries were constructed using the VAHTS Universal V6 RNA-seq Library Kit for MGI (Vazyme) following the manufacturer's recommendations with unique index codes. The Library quantification and size was assessed using a Qubit 3.0 Fluorometer and Bioanalyzer 2100 system.