RNA-seq of G. intestinalis clonal population treated with mAb to induce antigenic variation
To minimize the heterogeneity of expressed VSPs in culture, clonal trophozoite populations were created from the original WB-C6 clone using serial dilution. Total RNA was purified from clonal population by Trizol extraction according to manufacturers protocol. Ribosomal RNA was depleted in all samples and strand-specific Illumina sequencing libraries were constructed. RNA sequencing was performed as a paid service by Genehub.com. The libraries were sequenced as 2x150 bp paired-end reads on an Illumina MiSeq instrument.