ANON2006Atypical
Single probe-positive CD19+ CD10- B cells were flow sorted from Malian PBMCs. The heavy chain of the BCR was amplified using reverse transcription and PCR followed by Sanger sequencing as previously described (Andrews et al., Immunity, 2019; Tiller et al., J. Immunol. Methods.  2008). Sequencing primers 3'IgGint GTT CGG GGA AGT AGT CCT TGA C and 3'IgMint GGA ATT CTC ACA GGA GAC GA.