GSM5065160: 4_15PCW_Retina [scRNA-seq]; Homo sapiens; RNA-Seq
Retinal tissue was excised from donated eyes and dissociated to single cells using a multi tissue dissociation kit (Miltenyi Biotec). Dissociation time varied from 15 to 45 minutes depending on the age of the tissue and a gentleMACS Dissociator (Miltenyi Biotec) was used to aid dissociation of the adult tissue. For scRNA-Seq libraries were generated using the Chromium Single Cell 3′ Library & Gel Bead Kit, version 3 (10x Genomics)