GSM5573291: SDI_12w_naive_1; Mus musculus; RNA-Seq
bronchioalveolar lavage fluid was collected (2x1ml PBS + 2mM EDTA) 4h after SDI/NanoPart administration and cells were spun down, resuspended in 100 µl Trizol. RNA was extracted using the DirectZol kit (Zymo Research) RNA-seq libraries were constructed using the Illumina TruSeq Stranded Total RNA Library Prep Kit.