Enriched genomic library of Campanula glomerata
Total genomic DNA was isolated from 10 individuals using X then equimolarly pooled. 1g was used for the development of DNA genomic library using the Illumina MiSeq platform to generate 250bp paired-end reads, following manufacturer's instructions from MiSeq Reagent Nano Kit v2.A total of X reads were obtained, merged in X contigs with Usearch software (Edgar, 2010). The bioinformatics program QDD v3 (Meglecz et al. 2014) was used to analyze sequences. QDD treats all bioinformatics steps from raw sequences until obtaining PCR primers: removing adapters/vectors, detection of microsatellites, detection of redundancy/possible mobile element association, selection of sequences with target microsatellites and primer design by using BLAST, ClustalW and Primer3 programs. X primer sets were finally designed on suitable microsatellite anking regions. A selection of X primer pairs was made, by keeping only perfect di/tri/tetra motives, with A and B quality design (from internal parameters of QDD), and with at least 20bp between primer and microsatellite.