RNA-Seq of S. anginosus BHI4 control rep1
To perform transcriptome sequencing (RNA-Seq) analysis, quality and integrity of the extracted total RNA was assessed by BioAnalyzer and the ribosomal RNA was removed by Ribo-Zero rRNA Removal Kit for Bacteria (Illumina). Standard illumina sequencing system protocol (TruSeq Stranded Total RNA prep) have been used to make libraries for RNA-Seq. Around 300 bp fragments were isolated using gel electrophoresis, amplified by PCR and sequenced on the Illumina HiSeq 3000 in the paired-end sequencing mode.