Metagenome of a TCE/Cr reducing biochatode
Sample DNA concentrations were measured using the Qubit dsDNA HS kit and the DNA qualitywas evaluated using TapeStation with the Genomic ScreenTape (Agilent Technologies). Sequencing librarieswere prepared using the NEB Next Ultra II DNA library prep kit for illumina (New England Biolabs, USA) followingthe manufacturers protocol. Library concentrations were measured in triplicate using the Qubit dsDNA HS kit and library size estimated usingTapeStation with D1000 HS ScreenTape. The sequencing libraries were pooled in equimolar concentrations anddiluted to 4 nM. The samples were paired end sequenced (2x301bp) on a MiSeq (Illumina, USA) using a MiSeqReagent kit v3, 600 cycles (Illumina, USA) following the standard guidelines for preparing and loading sampleson the MiSeq.