GSM5381203: iPSST_1; Homo sapiens; RNA-Seq
Global gene expression profiles using RNA-seq were performed in two replicates of primary hTSCs (CT27), undifferentiated hESCs (H9), undifferentiated hiPSCs, hTSLCs (TS-like cells) derived from hESCs (ES-TSLCs), hTSLCs derived from hiPSCs (iPS-TSLCs), EVT differentiated from hTSCs, EVT differentiated from ES-TSLCs (ES-EVT), EVT differentiated from iPS-TSLCs (iPS-EVT), ST differentiated from hTSCs, ST differentiated from ES-TSLCs (ES-ST), and ST differentiated from iPS-TSLCs (iPS-ST). Briefly, total RNAs were isolated using the RNeasy plus minikit (Qiagen). mRNAs were enriched from total RNAs, fragmented, and primed. First strand cDNAs were synthesized following by second strand synthesis. The ends of purified double strand cDNAs were repaired, ligated with a barcoded adaptor and index, and amplified with PCR. Purified final PCR product was sequenced using Illumina Novaseq 6000 machine. RNA-seq library was constructed using NEBNext Ultra RNA Library prep kit (NEB, E7700L) according to manufacturer's instruction.