GSM5495100: Fetal chondrocytes2; Homo sapiens; ATAC-seq
Samples were washed, lysed followed by nuclei tagmentation and adapter ligation by Tn5 using the Nextera DNA Sample Preparation kit (Illumina). Transposed DNA fragments were amplified using the NEBNext Q5 HotStart HiFi PCR Master Mix with regular forward and reverse barcoded primers. The final product was purified with MinElute PCR Purification kit (Qiagen), and quality checked on 2100 Bioanalyzer (Agilent). Sequencing was performed on Illumina HiSeq 2500 with single-end 50 base pair reads. Nextera DNA Sample Preparation kit