GSM6297424: HIF-2a/1a, IgG, rep2 [CUT&Run]; Homo sapiens; OTHER
Chromatin was digested with final 0.1% SDS and 0.25 mg/ml Proteinase K for 1h at 50 °C, and extracted once with equal volume of Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v) (Invitrogen) and then with equal volume of chloroform (Fisher) Up to 40 ng of purified DNA was used for preparing library, using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®  (NEB E7645)