Shotgun sequencing metagenomic: water samples from Lake Cajititlan
Each replicate of the water samples was first filtered using a membrane with pore sizes ranging from 20 to 25 ?m. After that, the filtrate was then passed through a second membrane with a pore size of 0.45 ?m. The filters were then cut into pieces, and 100 mg of each was placed in a lysis matrix to do DNA extraction and purification of the samples using the FastDNA Spin Kit for Soil (MP Biomedicals, OH, United States), following to the manufacturer's protocol. Quantification of DNA was conducted using a NanoDrop ND-1000 UV-Vis spectrophotometer (NanoDrop Technologies, Wilmington, DE). Therefore, a pool of DNA sample sets was made by sampling months, ultimately obtaining a total of 30 samples (15 samples plus replicas). The samples were then cleaned using the AM Pure XP kit (Beckman Coulter, IN, USA), and their DNA was quantified using the Qubit 2.0 fluorometer (Invitrogen, Carlsbad, CA, USA). Additionally, the absorbance ratio at 260/280 and 260/230 nm was measured to analyze the purity of the samples. Finally, the samples were subjected to shotgun metagenomic sequencing on the Illumina Novaseq6000 platform (Illumina, San Diego, CA, USA), yielding paired-end read lengths of 2 x 150 bp and 5 Gb of clean data per sample.