GSM5832066: single cell murine FTE cells and OSE cells; Mus musculus; RNA-Seq
To isolate fallopian tube (FT) cells, the distal portion of FT was dissected, minced, and enzymatically digested. After the enzymatic reaction, FT cells were passed through a 40 um cell strainer to achieve a single cell suspension. To isolate ovarian surface (OS) cells, ovaries were dissected and incubated with Trypsin-EDTA to strip OS cells. Stripped OS cells were then passed through 100 um and followed by 40 um cell strainers to achieve a single cell suspension. Barcoded single cell RNA-seq library was generated by the Harvard Medical School Single Cell Core (https://singlecellcore.hms.harvard.edu/), following the instructions of the InDrop Design V3.