metagenome of deep-sea hydrothermally inactive sulfides
About 100 ng genomic DNA was fragmented to 350 bp by Covaris M220 Focused-ultrasonicator (Woburn, MA, USA). Then metagenomic libraries were performed with reference to the instructions of TruSeq Nano DNA Library kit (Illumina, San Diego, CA, USA). The libraries were sequenced on the Hiseq 2000 platform via 2100 bp paired-end sequencing (Illumina, San Diego, CA, USA).