GSM4848174: H3wt_ATAC-seq_Rep2; Drosophila melanogaster; ATAC-seq
[ATAC-seq]  Crude nuclear extract was prepared in ATAC lysis buffer (10 mM Tris pH 7.4, 10 mM NaCl, 3 mM MgCl2 0.1% IGEPAL), followed by tagmentation using Tn5 Transposase (Illumina, 15027865). Tagmented DNA was purified using Agencourt AMPure XP beads (Beckman coutler, A63880). [ATAC-seq]  Following tagmentation DNA was amplified using primers from Nextera Index Kit. [CUT&Tag]  Crude nuclear extract was prepared in Nuclear Extraction buffer (20 mM HEPES pH 7.9, 10 mM KCl, 0.5 mM spermidine, 0,1% Triton X-100, 20% glycerol) followed by binding to Concanavalin A-coated beads (Polysciences, Inc. 86057-3) and incubation with primary antibody. Tagmentation was performed using Protein A-Tn5 fusion protein. [CUT&Tag]  Following tagmentation DNA was amplified using primers from Nextera Index Kit.