GSM5573062: liver tissue-vehicle-GH002; Mus musculus; RNA-Seq
Frozen liver samples were submitted to Columbia Molecular Pathology/MPSR (HICCC) core where they were homogenized with Qiagen TissueLyser II, at 25/s oscillation frequency for 2 min. RNA was extracted with Qiagen miRNeasy mini kit following the instructions of the manufacturer, and RNA was eluted at a volume of 50 ul. RNA samples with RIN 8≥ were submitted to the JP Sulzberger Columbia Genome Center for RNAseq analysis. Poly-A pull-down was used to enrich mRNAs from total RNA samples, followed by library construction using Illumina TruSeq chemistry. Libraries were sequenced using Illumina NovaSeq 6000. Samples were multiplexed in each lane, yielding a targeted number of paired end 100-bp reads for each sample.