GSM5287269: CTCF KO mouse 1, sample A; Mus musculus; RNA-Seq
iCTCFpod-/- and WT mice were sacrificed after 1 week of doxycycline treatment and kidneys were quickly dissected and washed with ice-cold Hanks' Balanced Salt Solution. Glomeruli were enriched using a sieving technique. Single cell suspensions were generated via enzymatic and mechanical dissociation. Single cells were processed through the 10X Chromium 3' Single Cell Platform following the manufacturer's protocol.