Illumina HiSeq 2500 paired end sequencing; RNA-seq of Delta47 human myeloma cells expressing either the FNDC3A protein or a Mock control
RNA-seq of Delta47 human myeloma cells expressing either the FNDC3A protein or a Mock control
Delta47 cell lines were grown in RPMI 1640 medium containing 100 U/ml penicillin and 100 microg/ml streptomycin supplemented with 20% FBS and 1% glutamine.  Total RNA from control delta47 cells and delta47 cells expressing FNDC3A were isolated using the RNeasy kit (Qiagen) Random primed cDNA libraries were constructed from 100 ng of total RNA.