Multi-barcode amplicon sequencing of Ophiostomatales mock community
PCR amplification of ITS1 and TEF1a barcodes perfromed in parallel reactions, with three technical PCR replicates each. Amplicons were cleaned and normalised in sequalPrep plates before being pooled back to sample. Libraries were prepared by attaching 8bp indexes to the sequencing adapters using qPCR. Amplicon libraries were again cleaned and normalised using SequalPrep, with final QC performed on the pooled library using a TapeStation and Qubit. Libraries were sequenced on an Illumina Miseq system.