SRA of Mycobacterium Smegmatis mc2155 8TA
8TA strain and M. smegmatis strain Mc2155 were incubated at 37C until OD600 reached 0.6 to 0.8. The chromosomal DNA of Mycobacterium smegmatis was extracted by the modified CTAB method. The concentration and integrity were detected by Qubit fluorophotometer and agarose gel electrophoresis respectively, and the sample quality was comprehensively judged according to the concentration, volume, total amount and electrophoresis results. After the library was constructed, Qubit 2.0 was used for preliminary quantification, the library was diluted, and then the Agilent 2100 was used to detect the inserts of the library. After the library is qualified, the illumina high-throughput sequencing platform NovaSeq 6000 is used for sequencing, and the original data volume is more than 1G , and approximately 200-fold coverage for the genome size was expected.