GSM4978107: miR_Atl_3; Cervus nippon; miRNA-Seq
Antlers were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. A total amount of 3 μg total RNA per sample was used as input material for the small RNA library. Sequencing libraries were generated using NEBNext®Multiplex Small RNA Library Prep Set for Illumina® (NEB, USA.) following manufacturer's recommendations and index codes were added to attribute sequences to each sample. the small RNA libraries were prepared for sequencing using standard Illumina protocols