GSM6260301: Lin-Kit+ hematopoietic stem and progenitor cells - heparan sulfate high; Mus musculus; RNA-Seq
FACS-purified HSPCs (Lin-c-kit+) sorted on HS scFv binding were used to prepare scRNA-seq libraries using the 10x Genomics Chromium 3' v3 kit according to manufacturer protocol (10x Genomics). Single cells were isolated using the Gemcode technology platform using barcoded droplets and paired-end reads of 150 bp. 10x Genomics Chromium 3' v3 kit. Libraries were submitted for sequencing to Novogene using an Illumina Hi-Seq platform with a target of 10,000 cells per condition.