Whole genome shotgun sequencing of canine: healthy adult feces
Stool samples were processed and sequenced in a single batch at Diversigen, Inc., USA. Briefly, samples were extracted with Zymogen Quick-DNA Fecal/Soil Microbe 96 Mag Bead kit (Zymo Research, USA) using Powerbead Pro (Qiagen, USA) plates with 0.5 mm and 0.1 mm ceramic beads. Extraction controls included were a no template control (water) and a characterized homogenized stool. All samples were quantified with Quant-iT Picogreen dsDNA Assay (Invitrogen, USA). Libraries were prepared with a procedure adapted from the Nextera DNA Library Prep (Illumina, USA).