GSM5770641: HiC_D0_3T3L1_siRNA_scrmb_1; Mus musculus; Hi-C
Hi-C libraries generated using Hi-C 2.0 protocol (Belaghzal et at.2017) and using the Arima-HiC kit, according to the manufacturers protocols. Flash frozen cross-linked cells were lysed and then digested with DpnII-containing enzyme mix at 37°C. The DNA overhanging ends were then marked with biotin followed by proximal end ligation. DNA was reversed crosslinked and ligation products were purified and then fragmented by sonication to an average size of 200-300 bp and size-selected to fragments of < 350 bp. We then performed end repair and Biotin-enrichment. For library construction, dA-tailing was performed on Hi-C samples followed by Illumina TruSeq adapter ligation. Samples were amplified by RT-PCR and libraries were purified.