RNA-Seq of mus musculus: adult female pancreatic islets
Islets were maintained in islet culture media after isolation (RPMI media with 11.1 mM D-glucose supplemented with 10% vol/vol fetal bovine serum and 1% vol/vol Penicillin-Streptomycin) at 37C, 5% CO2. Islets were flash frozen on dry ice in 100 L of RLT buffer with beta mercaptoethanol (1%). RNA was extracted with RNeasy. Quality control of samples was performed using the Agilent 2100 Bioanalyzer to confirm sample quality. RNA libraries were prepared for sequencing using standard Illumina protocols.