GSM5742652: PTG KO ZT8 rep2 [#30 KO 14:30]; Mus musculus; RNA-Seq
Parathyroid glands were harvested, flash frozen in liquid nitrogen and store at -80 degrees. Samples are pooled from two male mice. Total RNA was isolated using Allprep DNA/RNA minikit (Qiagen, Hilden, Germany) and purified using Qiagen RNase-Free DNase Set (Qiagen, Hilden, Germany) on a QIAcube Connect (Qiagen, Hilden, Germany). RNA quality was examined using an Agilent Technologies 2100 Bioanalyzer. Library preparation was done using a TruSeq Stranded Total RNA Gold kit with dual adaptor indexing strategy; IDT for Illumina – TruSeq RNA UD Indexes (Illumina, San Diego, USA). The library was diluted and pooled to a concentration of 2 nM according to the procedure of “Denature and Dilute Libraries Guide of Illumina Novaseq 6000” and final loading concentration was 400 pM. RNA libraries were prepared for sequencing using standard Illumina protocols