GSM5381304: Mutant Replicate 3; Mus musculus; RNA-Seq
Individual pancreata were dissected and flash frozen on dry ice. RNA was harvested from pools containing 5-6 pancreata of the same genotype using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit was used with 2-3 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols