GSM5331321: DP1; Mus musculus; RNA-Seq
100 sorted cells from bone marrow and spleen were extracted in lysis buffer with NP40 and Rnase inhibitor. The first strand of cDNA was synthesized using a PrimeScript RT reagent kit (TaKaRa Bio Inc.) and not-so random primers. Following the synthesis of the first strand, the second strand was synthesized by Klenow Fragment (3', -5', exo-; New England Biolabs Inc.) and complement chains of the not-so random primers. Using purified double-strand cDNA, the library for RNA-Seq was prepared and amplified using a Nextera XT DNA sample Prep Kit (Illumina Inc.).