ChIP-seq_input
ChIP-DNA was extracted with phenol:chloroform:isoamyl alcohol (SigmaAldrich, P3803), precipitated with ethanol, and resuspended in TE buffer. ChIP DNA libraries were prepared using an NEBNext Ultra II DNA library prep kit for Illumina (New England BioLabs, E7645). Briefly, ChIP DNA was end-repaired, ligated with an adaptor, and followed by 610 cycles of PCR amplification, per the manufacturers guidelines. Next, library fragments of 250650 bp were selected using AMPure XP beads (Beckman, A63881). Finally, the DNA fragments were sequenced using an Illumina HiSeq X Ten system (paired-end 150-bp reads)