GSM5145331: WT-H4K5ac-Input; Arabidopsis; ChIP-Seq
Lysates were clarified from sonicated protein-DNA complexes were isolated with GFP antibody. Two ng of ChIP DNA immuoprecipitated by anti-GFP antibody was used for ChIP-seq library construction.  Libraries were constructed by the VAHTSTM Universal DNA Library Prep Kit for Illumina® V3 (ND607), VAHTSTM DNA Adapters set3 - set6 for Illumina® (N805) and VAHTS® DNA Clean Beads (N411-02, Vazyme) according to the manufacturer's protocol. protocol. Illumina Novaseq was used for high-throughput sequencing of the ChIP-seq library.