GSM5618618: WT membrane monosomes, rep2; Escherichia coli; OTHER
Polysomes were digested with MNase (3750 U/1 mg RNA), purified through a sucrose cushion, and affinity purified via streptavidin magnetic beads . RNA from total monosomes and SecA-bound monosomes was extracted using Direct-zol kit. Ribosome-protected fragment were size selected and converted into a cDNA library for sequencing. Libraries are prepared as described in (Mohammad and Buskirk, 2019) with modifications.