GSM5065643: Perturb_CD4_stim_intra_D6_ATAC; Homo sapiens; ATAC-seq
Samples were stained with hashing antibodies for multiplexing gRNAs and surface marker antibodies, fixed with 1% PFA and then extracted using the 10x protocol (Omni-ATAC) ATAC libraries were constructed according to the 10x Genomics scATAC-seq v1.1 protocol while antibody tags were captured via the bridge oligo spiked-in the barcoding reaction and amplfied using tag-specific handles. 10x Genomics scATAC-seq v1.1