GSM4978163: sRNA K562i WT clone 1; Homo sapiens; miRNA-Seq
Cells were washed with PBS, and RNA was harvested using TRI-Reagent. Small-RNA sequencing libraries were prepared as described (Kingston and Bartel, 2019).  A step-by-step protocol is available at http://bartellab.wi.mit.edu/protocols.html. Libraries were prepared from 5 ug total RNA extracted from cells.