WGS of Rhipicephalus microplus (clade A) from Laos
DNA was isolated from dried whole tick using DNA Isolate II kit (Bioline, Australia) according to manufacturers instructions and eluted in 100 ul of Tris buffer (pH = 8.5). DNA was stored at -20 C. Isolated DNA was used for library construction followed by the next generation sequencing using 150bp paired end Illumina sequencing system at the depth of 1Gb of raw sequence data (Novogene, Singapore).