RNA-seq of Drosophila melanogaster Oregon-R Whole Embryos
Embryos were homogenized using a Pellet Pestle Cordless Motor (Kimble Cat. No. 749540-0000; Pellet Pestles Sigma  Cat. No. Z359947), RNA was extracted using TRIzol Reagent (Thermo Fisher, Cat. No. 15596026) and purified with the RNeasy Mini Kit (QIAGEN Cat. No. 74106). Libraries were constructed with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina according to manufacturers recommendations (NEB, cat. no. E7420).