GSM5993874: STZ+SGLT2i [SGL2]; Mus musculus; RNA-Seq
RNA was extracted from renal tissue using RNeasy Mini Kit (QIAGEN, Germany) and RNA concentration was measured using nanodrop (2000C, Peq lab, Germany). The quality and integrity of RNA were controlled with an Agilent Technologies 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany). The RNA sequencing library was generated from 500ng total RNA using Dynabeads® mRNA DIRECT™ Micro Purification Kit (Thermo Fisher) for mRNA purification followed by NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England BioLabs) according to manufacture´s protocols.