Amplicon of Severe acute respiratory syndrome coronavirus 2: human tracheal aspirate ICU-TA-113-496-Ampliseq
The ICU-TA-113-496-Ampliseq library was prepared using the AmpliSeq Library Plus and cDNA Synthesis for Illumina kits (Illumina), with 20ng input, amplified with the Illumina SARS-CoV-2 research panel (Illumina), ligated with AmpliSeq CD Indexes, enriched with 18 cycles of PCR, and sequenced on a NovaSeq6000 with 2x150bp reads