GSM5618045: case3-be; Homo sapiens; ncRNA-Seq
Total RNA was extracted using the TRIzol reagent. RNA sample was then pretreated to remove some RNA modifications that interfered with small library construction using the tRF&tiRNA Pretreatment Kit. The pretreated total RNA samples were then subjected to library preparation using the NEBNext Multiplex Small RNA Library Prep Set for Illumina kit. The completed libraries were then sequenced on Illumina NextSeq 500 system. Sequencing libraries are size-selected for the RNA biotypes to be sequenced using an automated gel cutter. The libraries are qualified and absolutely quantified using Agilent BioAnalyzer 2100. tRF-seq