GSM4978183: sRNA S2 WT clone 3 TUBE input; Drosophila melanogaster; miRNA-Seq
Cells were washed with PBS and lysed in TBS-N buffer (10 mM Tris pH 7.5, 150 mM NaCl, 0.5% NP-40, supplemented with protease inhibitor). For input samples,RNA was extracted from lysates using TRI-Reagent.  For eluates, lysates were incubated with anti-FLAG magnetic beads.  Beads were washed with TBS-N, and RNA was eluted and isolated using TRI-Reagent. Small-RNA sequencing libraries were prepared as described (Kingston and Bartel, 2019).  A step-by-step protocol is available at http://bartellab.wi.mit.edu/protocols.html. Libraries were prepared from RNA extracted as described.