GSM5380918: week-10_rep1 [840-RNA_L8]; Mus musculus; RNA-Seq
Around 40 mg tissue was used for RNA extraction using Ultrapure RNA Kit (CWBIO, CW0581M). Briefly, tissues were triturated for 30s in 1 mL TRIzon provided in the kit, incubated at room temperature for 5 min, added with 200 μL chloroform and shaken drastically. After centrifugation at 12000 rpm, 4 °C for 10 min, the upper water phase was moved into an adsorption column provided by the kit. The column was then eluted with 50 μL RNase-free water. RNA-seq libraries were constructed by NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB E7490) and NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module (NEB E6111) RNA libraries were prepared for sequencing using standard Illumina protocols