GSM5212815: SG_MEF_2; Mus musculus; RNA-Seq
Total RNA ((including gDNA removal) was extracted using by RNeasy plus mini kit (Cat no.#74134, Qiagen). Sequencing libraries were prepared using TruSeq RNA Sample Preparation kit v2 (Illumina).  After pooled libraries were denatured, sequencing of each library was carried out using the 100 bp. paired-end mode of the TruSeq Rapid PE Cluster Kit and TruSeq Rapid SBS Kit with HiSeq 2500 (Illumina Technologies., USA).