GSM5618435: Tri-1-T_PD1-/KLRG1-; Mus musculus; RNA-Seq
TILs were isolated by digesting tumor fragments using Mylteni mouse tumor disassociation kit with enzyme R at 1:10 per manufacturer's guidelines. 1 to 5 × 106 cells were stained using viability dye and fluorescently labeled mouse anti-CD45, anti-CD3, anti-CD8, anti-PD-1, anti-KLRG1 antibodies to sort 4 different CD8+ T cells populations based on PD-1 and KLRG1 expression (+ or -)  using the FACSAria II cell sorter.