GSM5333124: MEFs_4E_4A3_rep1; Mus musculus; RNA-Seq
eIF4E+/+ and eIF4E+/- MEFs expressing scramble, Magoh, eIF4A3 and Rbm8a sgRNAs were induced with Dox for 48 hours in triplicates, total RNA was isolated by Trizol and ribosomal RNA was removed by Ribo-Zero (Illumina). RNA seq libraries were prepared by ScriptSeq v2 RNA-seq library preparation kit (Epicenter) following the manufacturer's protocol and sequenced as paired-end 100 bp on HiSeq 4000