GSM4850500: H0193_well_D21_1; Homo sapiens; RNA-Seq
Total RNA was isolated with the RNeasy Micro kit (Qiagen). Briefly, MH cells were washed once with PBS 1x and collected in 350 μL of RLT buffer at room temperature. Three microfluidic channels were pooled for each biological replicate. RNA was then purified according to manufacturer's instructions. Total RNA was quantified using the Qubit 2.0 fluorometric Assay (Thermo Fisher Scientific). Libraries were prepared from 100 ng of total RNA using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen GmbH). Quality of libraries was assessed by using screen tape High sensitivity DNA D1000 (Agilent Technologies).