454 genomic of Talpa occidentalis : adult spleen
Library was prepared with the Rapid Library Preparation Method Manual according to the manufacture instructions. Briefly, DNA of high molecular weight was fragmented by nebulization. After fragment end repaired, blunt adapters were ligated to the DNA for subsequent amplification. One of the adapters contain a biotin molecule to bind to the coated-streptavidin AMpure beads. The non-biotinylated strains are removed, and the ones attached are used for amplifying by PCR the single-strain library, which it will be load into the instrument for sequencing.