GSM5618874: Group7_CD4cells_rep24; Homo sapiens; miRNA-Seq
FACS sorted BSLB were lysed and RNA was purified using miRNA advance kit microRNA libraries were prepared following NEBNext Small RNA kit (NEB) and the low yield protocol Libraries were amplified (15 cycles) on a Tetrad (Bio-Rad) using in-house unique dual indexing primers and size selection was performed using Pippin Prep instrument (Sage Science) using the 3% Agarose, dye free gel with internal standards (size selection: 120 to 230bp).