GSM5743388: MNK3_WT_ATAC, 2; Mus musculus; ATAC-seq
Fifty thousand cells were pelleted at 550 x g and washed with 50 μL ice-cold 1X PBS, followed by treatment with 50 μL of lysis buffer (10 mM Tris-HCl [pH 7.4], 10 mM NaCl, 3 mM MgCl2,0.1% IGEPAL CA-630). Nuclei pellets were resuspended in a 50 μL transposition reaction containing 2.5μL Tn5  transposase (FC-121-1030; Illumina). The reaction was incubated in a 37°C heat block for 45min. Tagmented DNA was purified using a MinElute Reaction Cleanup Kit (Qiagen) and amplified with varying cycles, depending on the side reaction results. Libraries were purified using a QIAQuick PCR Purification Kit (Qiagen). Libraries were validated for quality and size distribution using a TapeStation2200 (Agilent). Libraries were paired-end sequenced (38bp+38bp) on a NextSeq 500 (Illumina) DNA libraries were prepared for sequencing using standard Illumina protocols