Forest land plot 3 Rosa Morada 18S
Metagenomic DNA was extracted using the Power Soil DNA Isolation Kit (MO BIO Laboratories, CA, USA) according to the manufacturers instructions. Fragments of the small subunit (SSU) rRNAs were amplified with nu-SSU-0817 and nu-SSU-1196 primers for micro-eukaryotes (Rousk et al., 2010). Primers contained the Roche 454 FLX adapters and 10-pb tags. Amplification conditions included a denaturation step at 95C for 10 min followed by 30 cycles at 95C for 40 s, at 55C for 40 s, at 72C for 50 s, and a final step at 72C for 10 min. Amplicon libraries were pooled and purified using the QIAquick PCR Purification Kit (QIAGEN, Germany).