NextSeq 500 sequencing; Genome-Wide analysis of FBN1 haploinsufficiency in h-PES10 and h-PES12 fusion human embryonic stem cells
Genome-Wide analysis of FBN1 haploinsufficiency in h-PES10 and h-PES12 fusion human embryonic stem cells
Cells were trypsinized and kept in -80 until RNA extraction. Colonies formed out of single cells were collected and plated on mouse feeder cells in 6-well plates.  RNA was extracted using NucleoSpin RNA Plus kit mRNA fraction of total RNA was enriched by pulldown of poly(A)-RNA. RNA sequencing libraries were generated using TruSeq RNA Library Prep Kit (Illumina) according to the manufacturer's protocol.