GSM6259947: KPP_6; Mus musculus; RNA-Seq
Total RNA was isolated from tumors with the RNeasy® mini kit ((#74104, Qiagen) according to the manufacturer's instructions. Messenger RNA was purified from total RNA using poly-T oligo-attached  magnetic beads. After fragmentation, the first strand cDNA was synthesized using  random hexamer primers followed by the second strand cDNA synthesis. The library  was ready after end repair, A-tailing, adapter ligation, size selection, amplification,  and purification.