GSM6043856: RNAseq_DMSO_1; Mus musculus; RNA-Seq
HSPCs isolated from bone marrow cells of Trp53-/- mice were transfected with retroviruses encoding shKmt2d or shRen. 48 hours later, GFP-positive cells were sorted by a BD FACSAria™ III Cell Sorter (BD Biosciences) for RNA-seq. Kmt2d knockdown and Kmt2d restored AML cells with doxycycline withdrawal for 4 days were harvested for RNA-seq. RNA libraries were prepared using standard Illumina protocols. Transcriptome sequencing was performed by an Illumina NovaSeq 6000 sequencing platform with 150 bp paired-end sequencing.Kmt2d knockdown and Kmt2d restored AML cells with doxycycline withdrawal for 4 days were harvested for ATAC-seq. Genome sequencing was performed using an Illumina NovaSeq 6000 sequencing platform. RNA-seq library construction HSPCs isolated from bone marrow cells of Trp53-/- mice were transfected with retroviruses encoding shKmt2d or shRen. 48 hours later GFP-positive cells were sorted by the BD FACSAria™ III Cell Sorter (BD Biosciences, San Jose, CA) for RNA-seq. Kmt2d knockdown and Kmt2d restored AML cells with doxycycline withdrawal for 4 days were harvested for RNA-seq. RNA libraries were prepared using standard Illumina protocols.