SMRT seq of Okra
RNA was selected for subsequent studies at OD260/280 >2.0, OD260/230 at 1.8-2.1 and RNA integrity number (RIN>8). The Iso-Seq library was prepared according to the Isoform Sequencing protocol (Iso-Seq) using the Clontech SMARTer PCR cDNA Synthesis Kit and the BluePippin Size Selection System protocol as described by Pacific Biosciences (PN 100-092-800-03). Sequencing was performed on a Pacbio Sequel instrument.