GSM5571662: MetS-Ct_85; Mus musculus; RNA-Seq
Livers were removed and flash frozen. RNA was isolated from liver samples using the NucleoSpin kit (Macherey Nagel) and quantified using a Nanodrop 2000. Dnase I treatment was performed as part of the protocol. RNA-seq libraries were constructed using NEBNext Ultra Directional RNA Library Prep Kit and NEBNext Mulitplex Oligos for Illumina (NEB).