GSM6045471: Case16_primary_cancer; Homo sapiens; miRNA-Seq
Total RNA was extracted using the miRNeasy FFPE Kit or miRNeasy Mini Kit (Qiagen, Hilden, Germany). Small RNA libraries were prepared using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs, Ipswich, MA). Then, PCR products were separated by electrophoresis (120 V, 60 min), and DNA fragments corresponding to 140–160 bp (the length of small non-coding RNA plus the adaptors) were recovered.