shRNA library screening of murine cells: microglia
The pooled shRNA library was constructed with the Horizon Discovery SMART vector lentiviral shRNA constructs expressing shRNAs. The library encompassed 18 pools targeting 21,924 genes. DNA was isolated from the collected cells and ooled barcodes were amplified using Illumina-adapted SMARTvector Indexing PCR primer Kit A and B, and subjected to next generation sequencing (Illumina).