PacBio long read sequencing of Candidatus Endoriftia persephone
sample of trophosome obtained from a female Riftia pachyptila collected at the Tica hydrothermal vent site (Alvin dive 4839, 9 50.398 N, 104 17.506 W, 2514 m depth, 2016) was separated from the host trophosome tissue as described in Polzin et al (2019). The DNA of the enriched endosymbiont fraction was extracted using the Quiagen Blood & Cell Culture DNA Mini Kit (#Cat No./ID: 13323) following the manufacturers instructions. The long-read metagenome library was prepared using the PacBio large-insert DNA protocol and the SMRTbell Express Kit. The sequencing was performed on a Sequel1.