V4 16S rRNA amplicon sequencing of PCR grade water: PCR negative control
Water was used for the construction of a 16S rRNA gene library following a the wet-lab protocol from the Schloss lab. Briefly, Illumina indexed reads were created using PCR amplification of the V4 region of bacterial 16S rRNA gene. No PCR amplicons were observed in the negative control samples using water as the DNA template. Amplified DNA was normalized using the SequalPrep Normalization Plate (Invitrogen), and pooled into a single library for each 96-well plate.