GSM5448673: 1L-sgGAL4_DMSO; Homo sapiens; RNA-Seq
LNCaPi cells were viral transduced with sgRNA targeting PTGES3 or control sgRNA for 3 days and selected with puromycin for pure population for 3 days. Post-selection RNA was extracted from the cells using the Zymo Quick-RNA extraction kit. QuantSeq 3'mRNA-Seq library prep kit FWD for Illumina was used to prepare the library as per the manufacturer's protocol. Quality control was performed by using the Agilent Bioanalyzer 2100 system and the samples were sequenced in Illumina HiSeq 4000. The entire set of the experiment was done in two biological replicates.