GSM6261573: mES, rep1; Mus musculus; RNA-Seq
Total RNAs were isolated using TRIzol reagent (Invitrogen) according to the manufacturer's instruction. Strand specific total RNA library preparation was performed using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (E7420S). The precipitated DNA samples were prepared for DNA deep sequencing according to manufacturer's guidelines (SWIFT, #21096).