Isoetes sinensis genome sequencing
Library preparation and quality assessment for Illumina X Ten PCR-free paired-end genome sequencing were performed according to the manufacturers protocol (Illumina, USA). Genomic DNA was fragmented and size-selected through agarose gel electrophoresis. The ends of selected DNA fragments were blunted with an A-base overhang and ligated to sequencing adapters. After quality control by Agilent 2100 Bioanalyzer and qPCR, all PCR-free libraries were sequenced on an Illumina X Ten platform with 150-bp paired-end sequencing strategy.