RNA-Seq of 3D hiPSC-CM
3D hiPSC-CM were pre-culture for 4 (D-4 to D0) days followed by 10 days (D0 to D10), changing FujiFilm iCell CM Maintenance Media every 2 days. 30-35 spheroids were collected immediately out of culture and put in lysis buffer on D10 (2 days after last media change) Total RNA was extracted with an Omega bio-tek E.Z.N.A Total RNA kit. Illumina Truseq mRNA non-stranded library protocol with 100 ng of RNA was used to prepare sequencing libraries following the manufacture the protocol with the exception of using half reactions.