GSM4977859: E15.5_STAU2_RIP_rep1; Mus musculus; RIP-Seq
RNA immunoprecipitation with Stau2 antibody (Abcam ab60724). RNA isolation and purification with acid phenol/chloroform extraction (also as described in Baroni, T.E., Chittur, S.V., George, A.D., and Tenenbaum, S.A. (2008)). The libraries were prepared using 'ScriptSeq™ Complete Kit (Human/Mouse/Rat)-Low Input (Cat. No. SCL24H) from Epicentre/Illumina using manufacturer's instructions. A maximum of 800 ng of DNA-free RNA was used for ribosomal RNA removal using Ribo-Zero rRNA removal kit. rRNA free samples were purified and proceeded with ScriptSeq v2 RNA-Seq Library Preparation reaction which included RNA fragmentation, cDNA synthesis and terminal tagging of cDNA at both ends. Purified cDNAs were PCR amplified using primers against the adapters at both ends. The reverse primers for PCR amplification were used from ScriptSeq™ Index PCR Primers (Set 1, Cat. No. RSBC10948) using manufacturer's instructions to barcode the libraries for multiplexing.