GSM5285155: WT-input; Mus musculus; ChIP-Seq
Chromatin immunoprecipitations were performed with the SimpleChIP® Plus Enzymatic Chromatin IP Kit (Cat# 9005, Cell Signaling Technology) according to the provided protocol. Micrococcal nuclease was used to digest DNA to 150-900 bp fragments. Libraries were prepared using KAPA HTP Library Preparation Kit from Roche. The ChIP DNA was quantified on the Qubit® 4.0 Fluorimeter from ThermoFisher. Samples were then end repaired, 3' ends adenylated and barcoded with multiplex adapters. Samples were amplified with 15 cycles of PCR and then size selected with Ampure XP beads.