GSM5533906: Control 3 - Cotreatment; Homo sapiens; miRNA-Seq
Having cells lysed with 1 ml Trizol reagent and centrifuged with 200 µL chloroform, total RNA was trapped in an aqueous phase, and precipitated by adding 80 μg glycogen and 500 μL isopropanol, and an overnight incubation at −20 °C. RNA was finally dissolved in nuclease-free water. QIAseq miRNA Library Kit and Illumina NextSeq 500 platform were used for small RNA library preparation and sequencing, respectively, by the Ramaciotti Centre for Genomics (UNSW Sydney, Australia).