WGS of Aedes aegypti : Adult female
Around 200 to 300 ng of amplified DNA (100 to 200 fmols) was end prepped and ligated to sequencing adapters using the SQK-LSK 109 kit. The EXP-NBD104 kit was used for barcoding the samples. The equal quantities of barcoded samples were pooled to 300 ng for final sequencing. At this stage the remaining libraries were stored at -80C.