Metagenome of various surfaces in the Moscow hospital
DNA extraction from swabs was performed using the DNeasy PowerSoil Kit (Qiagen, USA) according to the manufacturer's instructions. Pure swabs, extraction reagents, and a sterile PBS solution were used as negative controls. The hypervariable V4 region of the bacterial 16S rRNA gene was amplified using the forward primer 515F, 5'-GTGCCAGCMGCCGCGGTAA-3' and the reverse primer 806R, 5'-GGACTACHVGGGTWTCTAAT-3'. Libraries were prepared using the NEBNext FastDNA Library Preg Set for Ion Torrent (New England Biolabs, USA) and barcoded with the use of Ion Code Barcode Adapters (Thermo Fisher Scientific, USA) according to the manufacturer's instructions. DNA sequencing performed using the Ion GeneStudio S5 System (Thermo Fisher Scientific, USA).