GSM6261024: Progeria‐AG11513‐P7‐R1; Homo sapiens; Hi-C
Briefly, 5-20 million cells were fixed with 1% formaldehyde, suspended in cell lysis buffer for permeabilization, and homogenized by douncing.  Samples were then digested overnight with HindIII or DpnII (as indicated per sample).  Ends were filled in with biotin and ligated.  Then, crosslinking was reversed. Biotinylated DNA was pulled down using streptavidin coated magnetic beads and prepared for multiplex sequencing on an Illumina platform using the NEBNext Ultra II kit (NEB). All end preparation, adaptor ligation, and PCR amplification steps were carried out on bead bound DNA libraries.