GSM6303890: Sample_18351; Homo sapiens; ATAC-seq
Cells were lysed in cold lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630) and tagmented with an in-house Tn5 in TAPS-DMF buffer Barcoded Nextera i7 and i5 primers were used to amplify the tagmented gDNA and preapare it for Illumina sequencing