GSM5532225: sgM3.3.2_Input; Homo sapiens; RIP-Seq
Total RNAs were isolated from the whole cells with QIAzol and poly(A) RNAs were enriched from total RNA with  oligo(dT) polystyrene beads.The anti-m6A antibody (202003, Synaptic Systems) was applied for m6A immunoprecipitation Both input and m6A IP samples were utilized for next-generation sequencing (NGS). The library preparation was constructed using the TruSeq Stranded mRNA Sample Prep Kit (Illumina) and was quantified by BioAnalyzer High Sensitivity DNA chip, and then was deeply sequenced on the Illumina HiSeq 2500. 