GSM5831747: WT.SpibKO.Sox8KO.AireKO.Pdpn-CD104-.mTEClo.rep1-4_HASH; Mus musculus; RNA-Seq
mTEC were prepared by digesting thymi with collagenase and dispase, magnetically depleting CD45+ cells, staining with fluorescent antibodies, hashing with TotalSeq antibodies against H2-Kb and CD29 (adult/perinate expt) or just H2-Kb (WT/Spib/Sox8/Aire expt), and flow sorting mTECs into DMEM/10%FCS. Purified cells were sent to the Broad Institute Genomics Platform, which performed encapsulation and RNA and hash library preparation following 10X Genomics protocols (3' v3 chemistry)