ATAC-seq of human: adult female peripheral blood mononuclear cells: Sample NS0010_ATAC
"ATAC-Seq libraries were generated and preprocessing was performed according to the protocol previously described. All samples were frozen in a DMSO/DMEM medium; cells were thawed for 1 minute in a 37 oC warming cabinet and transferred to a 50 mL Falcon tube. Cells were then suspended in 20 mL of warm 1x PBS followed by a 5-minute centrifugation at 1000 rpm to pellet the cells. Cells were lysed with a cold lysis buffer and spun to pellet the nuclei. Two million unfixed nuclei were tagged using a Tn5 transposase reaction mix (Illumina Nextera DNA Library Prep Kit) for 30 min at 37 oC, and the resulting library fragments were purified using the Qiagen Minelute kit. Libraries were amplified with 10-12 PCR cycles and finally sequenced on an Illumina HiSeq 2500, generating paired end 50 bp reads. Nuclei processing and sequencing were performed by the Applied Bioinformatics Laboratory at New York University."