GSM5743095: 20 DPH male RA 2; Taeniopygia guttata; RNA-Seq
Brains were removed, flash frozen in a slurry of dry ice and isopropanol, cryosectioned onto PEN membrane glass slides (Leica, No. 11505158), fixed in 100% cold ethanol, and stained using cresyl violet. Brain nucleus RA was extracted from PEN slides into RLT buffer (Qiagen) using laser capture microdissection (Leica LMD 6500). RNA was isolated using the RNeasy Micro Kit (Qiagen) with on-column DNase digestion. Using the SmartSeq v4 PLUS Kit (Takara Bio USA), cDNAs were generated from 1 ng total RNA , then the resulting cDNA was bioanalyzed on a High Sensitivity DNA chip, and 5 ng was used as input for the libraries.