GSM6261351: Control-8-MAP2; Homo sapiens; RNA-Seq
Fresh-frozen tissue from the prefrontal cortex (Brodmann area 9; BA9) of healthy control donors was thawed on ice, and the cortical ribbon spanning all cortical layers was micro-dissected. Gentle mechanical dissociation without detergents or enzymatic digestion followed by sucrose-iodixanol gradient centrifugation was used to purify cells with relatively well-preserved somas. Immunostaining and fluorescence-activated cell sorting using the pan-neuronal marker MAP2 was used to collect neuronal somas. RNA capture and library preparation was performed using the 10× Genomics Chromium Single Cell 3' v2 or v3 assay.