GSM6001984: 5KO,DS,BS,rep1; Mus musculus; Bisulfite-Seq
500 ng of genomic DNA was sheared by sonication to 100-500 bp using a Covaris S220 and purified by AMPure XP beads (Agencourt Bioscience Corp.). Genomic DNA libraries were constructed as ChIP-seq library. After adapter ligation, the DNA was treated with sodium bisulfite using EZ DNA Methylation GOLD kit (ZYMO RESEARCH) following the manufacturer's instructions. Enrichment for adapter-ligated DNA was carried out through 10 PCR cycles using Pfu Turbo Cx Hotstart DNA polymerase (Agilent).