GSM6300669: Black_Hepatocytes_ATAC_Seq; Homo sapiens; ATAC-seq
ATAC-Seq sample preparation and sequencing was conducted by ActiveMotif® (Carlsbad, CA, USA). Their procedure is summarized here with their permission. Cryopreserved cells were thawed in a 37°C water bath, pelleted via centrifugation, washed with ice-cold PBS, and tagmented (following Buenrostro et al., 2013) with modifications (based on Corces et al., 2017). Cells were resuspended in lysis buffer, centrifuged to pellet the debris. DNA was tagmented using the Nextera® Library Prep Kit (Illumina). The resulting DNA was purified via the MinElute™ PCR purification kit (Qiagen), amplified by PCR (ten cycles), and then re-purified with Agencourt AMPure SPRI beads (Beckman Coulter). The eluted samples were then quantified using the KAPA library quantification kit for Illumina (Roche) and sequenced via PE42 sequencing on a NextSeq® 500 sequencer (Illumina).