GSM5618493: A08_RNASeq, biological rep3; Saccharomyces cerevisiae; RNA-Seq
Total RNA was extracted using RNAeasy Mini Kit with a FastPrep homogenizer to disrupt cells. RNA was prepared for sequencing using the Illumina TruSeq samples preparation kit v2, with poly-A enrichment. The fragments were clustered on cBot and sequenced on a single lane on a HiSeq 4000 with paired ends (2 × 150 bp), according to the manufacturer's instructions. The number of read pairs obtained for each sample ranged from 12.0–16.0 million.