GSM5448648: DTR_GFP(-)_SATB2; Mus musculus; ChIP-Seq
10x5 cells were attached to Concanavalin A conjugated magnetic beads, permeabilized, and incubated with anti-SATB2 or isotype control antibodies at 4C for overnight. pAG-MNase (1:1000) was added in digitonin buffer to bind with antibodies. Finally, targeted chromatins were digested and released into the supernatant. DNA was purified with a MinElute purification kit. Libraries were prepared using the ThruPLEX DNA-Seq Kit Exactly followed the protocol from Takara bio (R400665). All final libraries were size selected (200-800 bp) by AMPure XP beads (Beckman, A63880) and loaded for sequencing.