GSM5949807: mESC_DMSO_2h_ATAC-seq_rep3; Mus musculus; ATAC-seq
Harvested cells were resuspended in 50µL CSK Lysis Buffer (10 mM PIPES, pH 6.8, 100 mM NaCl, 300 mM sucrose, 3 mM MgCl2, 0.1% Triton-X-100), incubated on ice for 5 min, and centrifuged for 5 min at 500 ´ g and 4ºC. Samples were tagmented for 30 min at 37ºC using TDE1 Tagment DNA Enzyme (Illumina).