GSM5570260: HUMN2; Homo sapiens; RNA-Seq
RNA extraction from paraffin embedded liver tissue. At least 4 human liver tissue-section cuts (≤ 10 µm thick) from the human control patients (HUMN1-3) were combined in an Eppendorf tube, after which a deparaffinization solution from the Quick-RNA FFPE Kit (Zymo Research, USA) was added and incubated at 55 °C for 1 minute. Subsequently, deparaffinized tissue was digested with proteinase K and digestion buffer from the Quick-RNA FFPE Kit (Zymo Research, USA) for 1 hour at 55 °C. After digestion, the Eppendorf tube was transferred and incubated at 94°C for 20 minutes. Further RNA extraction was performed using the same Quick-RNA FFPE Kit (Zymo Research, USA) according to the manufacturer's instructions.  RNA extraction from full liver tissue  RNA was extracted from human liver tissue preserved in RNAlater (ThermoFisher Scientific, Belgium) using the Aurum Total RNA Mini Kit (Bio-Rad Laboratories, Belgium), following manufacturer's protocol. Quantification and purity of the isolated RNA were determined via spectrophotometric analysis with a Nanodrop spectrophotometer (ThermoFisher Scientific, Belgium). Library construction was performed with the QuantSeq 3' mRNA FWD kit (Lexogen) and UMI Second Strand Synthesis Module (Lexogen).