GSM6001962: S0109; Mus musculus; RNA-Seq
To dissociate resected tumors into single cells, a 1 mL pre-warmed Papain solution at 2 mg/mL was added, and samples were incubated for 20 minutes at 37 degrees Celsius on a heating block with gentle shaking. Wide bore pipette tips were used to homogenate the solution with pipetting, and the brain tumor homogenates were subsequently mixed with 5 mL 10X buffer. Samples were passed through 100 micron followed by 70 micron filters and then counted. 10x Genomics Chromium Single Cell 3' v3.1 single-index kit was used according to the manufacturer's protocol.