GSM5284856: wildtype-vc0513_rep1; Vibrio cholerae O1 biovar El Tor str. N16961; RNA-Seq
Cells were harvested via centrifguation, resuspended in 1X phosphate buffered solution, and treated with RNA protect (Qiagen). mirVana™ miRNA Isolation Kit (Ambion) was used according to manufacturer instructions. Total RNA was treated with DNAfree (Ambion) and precipitated with ethanol and ammonium acetate. Ribosomal RNA was subtracted by hybridization from total RNA samples using the NEBNext rRNA Depletion Kit (New England Biolabs). TruSeq-barcoded RNAseq libraries were generated with the NEBNext Ultra II [Directional] RNA Library Prep Kit (New England Biolabs).