Neocamacolaimus parasiticus transcriptome
Total RNA was extracted from a single juvenile using the Ambion RNAqueous Micro Kit following the manufacturers protocol. Subsequent library preparation and cDNA synthesis was performed using the Takara Bio SMART-Seq HT Kit following manufacturers instructions. Resulting double-stranded cDNA was purified using AMPure XP for PCR purification kit. Final library preparation and transcriptome sequencing were performed at Macrogen Europe B.V the Macrogen using the Illumina Nextera DNA XT library preparation protocol and an Illumina HiSeq X sequencing technology (150PE).