GSM5770278: wild-type biological rep1; Mus musculus; RNA-Seq
Gastrocnemius muscle  were harvested and flash frozen on liquid nitrogen, and RNA was harvested using Trizol reagent. NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) was used with 1.5 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols.