GSM5444692: Tau_1_PS19(P301S)_PFC_ChIP-Seq; Mus musculus; ChIP-Seq
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with anti-H3K4me3 (ab8580, Abcam, 5 ug/reaction). Purified DNA was used to construct sequencing libraries with 5 ng of DNA (ChIP or input). Libraries were prepared using the Rubicon ThruPLEX DNA-seq Library Prep Reagent Set for Illumina sequencing according to the manufacturer's recommendations. DNA quality was measured with an Advanced Analytical Fragment Analyzer with a high sensitivity chip. 50 bp single end reads per sample were obtained using the HiSeq 2500 platform from Illumina.