GSM6261460: HMLE-TWIST1_200nm-D2_2; Homo sapiens; RNA-Seq
Total RNA was extracted using Trizol (Invitrogen) following the manufacturer's protocol and concentrated by ethanol precipitation. Column purification of 50µg of extracted RNA was performed using RNeasy Mini kits (Qiagen, 74106), with minor modifications to the manufacturer's protocol, including an extension of on-column DNase treatment from 15 to 30 minutes. For each sample, 1µg of RNA was used to generate libraries using SMARTer Stranded Total RNA Sample Prep kits (Takara, 634876), with integrated RiboGone enzymatic rRNA depletion. RNA libraries were prepared for sequencing using SMARTer Stranded Total RNA Sample Prep kits (Takara, 634876) protocols