GSM5597267: sex converted XX MII oocytes rep1; Mus musculus; RNA-Seq
PolyA+ RNAs were purified from oocytes (GV: 31-158; MII: 35-58), using a Dynabeads mRNA DIRECT Micro Kit (Invitrogen). Biologically duplicated samples in each stage were prepared. Purified RNAs were subjected to library preparation using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). cDNAs were amplified by 13-cycle PCR. The libraries were sequenced on NextSeq 500 (75 bp single, Illumina).