RNA-seq of Setaria viridis with aphid feeding
Tissues from 4-6 plants were combined into one replicate, and four replicates were collected for each treatment (aphid and untreated control). Total RNA was extracted using an SV Total RNA Isolation Kit with on-column DNase treatment (Promega) [27]. For transcriptomic analysis, strand-specific RNA-seq libraries were prepared [61], [62]. The purified RNA-seq libraries were quantified, and 20 ng of each was used for next-generation sequencing using an Illumina HiSeq2000 instrument at Weill Cornell Medical School with a 101 bp single-end read length.