47C
DNA was extracted using phenol:chloroform and ethanol precipitation . High molecular weight DNA (>20kb) was isolated using a Blue Pippin (Sage Science, Beverly, MA, USA) with a High Pass Plus Casette. Nanopore libraries were generated with the SQK-LSK109 1D Genomic Ligation kit (Oxford Nanopore, Oxford Science Park, UK). Reads were generated with a R9.4.1 flow cell using a 48-hour script. Reads were basecalled with guppy v2.3.1+1b9405b6.