GSM6216581: pleomorphic adenoma portion of carcinoma ex pleomorphic adenoma, PA10; Homo sapiens; miRNA-Seq
RNA was harvested using Trizol reagent (Invitrogen). Exosomal RNA was isolated using TRIzol LS reagent (Ambion). Total RNA was extracted from 200㎕ serum/plasma using miRNeasy Serum/Plasma Kit (Quaigen). Total RNA was used for the construction of sequencing libraries. RNA libraries were prepared for sequencing using NEBNext Multiplex SmallRNA Library Prep Kit (New England BioLabs) standard protocols. small RNA-seq