GSM6044945: DMSO_TC00000651_A22; Homo sapiens; RNA-Seq
Twentyfour hours after chemical treatment, HTTr assay plates were removed from the incubator and media in each assay well was drained to a residual volume of 10 µL using MultiFlo FX microdispenser equipped with a vacuum-driven aspiration manifold. To create cell lysates, 10 µL volumes of 2X BioSpyder lysis buffer were dispensed into each assay well using the same MultiFlo FX instrument equipped with 1 µL peristaltic pump dispensing cassette. Plates were temporarily sealed with an adhesive film, shaken for 30 s on the stage of MultiFlow FX microplate dispenser (BioTek, Winooski, VT) and then placed in a hybridization oven (37°