Amplicon sequencing: ITS of bulked pollen
The nuclear ITS region was amplified with primers ACCTTATCATTTAGAGGAAGKARAARTCGTAACAAGGT and TTCCTCCGCTTATTGATATGCTTAARYTCAGC as decribed by Cornman et al. 2015 (doi.org/10.1371/journal.pone.0145365), producing non-overlapping read pairs