GSM6260949: SBA1; Homo sapiens; RNA-Seq
When large colonies of amniotic fluid cells appeared at passage 0, the cells were detached with trypsin/EDTA (Gibco) for 5 min and resuspended in Chang C complete medium supplemented with 0.5% BSA (w/v) (Gibco) at a concentration of 1,000 cells per µL. The quality and concentration of the single-cell preparations were evaluated using a hemocytometer. Samples were immediately loaded into the 10X Genomics Chromium controller. Approximately 17,000 cells were loaded per sample. The 10X Genomics libraries were prepared according to the manufacturer's instructions (10XGenomics protocol Single Cell 3' v3). Libraries were sequenced in an Illumina NovaSeq sequencer according to the 10X Genomics recommendations to a depth of around 50,000 reads per cell. Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry) 10x scRNAseq