GSM5380070: WT; Caenorhabditis elegans; RNA-Seq
Animals were washed off the plates with M9, frozen in QIAzol using ethanol/dry ice, and stored at -80˚C prior to RNA extraction. Total RNA was extracted using the RNeasy Plus Universal Kit (Qiagen, Netherlands). Residual genomic DNA was removed using TURBO DNase (Life Technologies, Carlsbad, CA). A total of 6 µg of total RNA was reverse-transcribed with random primers using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA).  Library construction and RNA sequencing on the BGISEQ-500 platform was performed and paired-end reads of 100 bp were obtained for subsequent data analysis.