GSM5770108: TBI_Cortex_c52 [miRNA-seq]; Rattus norvegicus; miRNA-Seq
RNA was extracted rom the perilesional cortex and ipsilateral thalamus using a mirVana miRNA isolation kit (#AM1560, Life Technologies (Ambion) Carlsbad, CA, USA), QIAshredder (#79654, Qiagen), and AllPrep DNA/RNA Mini Kit (#80204, Qiagen). Thereafter, RNA purification was performed using miRCURY™ RNA Isolation Kit (#300111, Exiqon, Vedbaek, Denmark). 300ng of total RNA was converted into microRNA NGS libraries using NEBNEXT library generation kit (New England Biolabs Inc.) according to the manufacturer's instructions. Samples were sequenced on the Illumina NextSeq 500 system.