GSM5285870: Sample13_15ADON, Norman, 96 hr, Replicate 1 [15-N-96-1]; Hordeum vulgare; RNA-Seq
Seeds were pulverized in liquid nitrogen using a mortar and pestle, and total RNA was extracted using a RNeasy Plant Mini Kit (Qiagen, Canada) following the manufacturer's protocol. Three biological replicates of Norman and CDC Kendall at 72 h and 96 h time-points, were subjected to RNA-seq analysis. The RNA quality and concentration were verified using a NanoDrop 1000 spectrophotometer (ThermoFisher Scientific, Canada) and an Agilent 2100 Bioanalyzer (Agilent Technologies, Canada) prior to sequencing. Sequencing libraries were generated using Illumina first-strand TruSeq® mRNA Prep Kit following manufacturer's recommendations and index codes were added to attribute sequences to each sample. Briefly, mRNA was purified from total RNA using oligo (dT)-attached magnetic beads. Libraries were sequenced on the Illumina HiSeq 4000 following the manufacturer's protocols.