GSM5145088: ZLM Rep2; Mycobacterium tuberculosis; RNA-Seq
Cultures were centrifuged at 4°C, and cell pellets were resuspended in TRIzol. TRIzol extractions were homogenized by bead beating and RNA was extracting following the manufacturer's protocol. Ribosomal RNA was depleted from the samples using Ribo-Zero plus rRNA depletion kit (Illumina®, Cat. #:20037135) before being sequenced on an Illumina® HiSeq platform for generation of 150 base pair paired end reads. RNA sequencing (RNAseq) was performed by GENEWIZ® (South Plainfield, NJ 07080).