GSM5993775: MDSC_SENE_ROMI_2; Mus musculus; RNA-Seq
RNA was then extracted using TRIzol RNA isolation reagent (Invitrogel, Cat n=15596026) and RNase-Free DNase set (Qiagen, Cat n=79254) treatment was performed following manufacturers' guidelines. RNA integrity was checked with Agilent 2100 Bioanalyzer system. Protocol used was NEBNext poly(A) mRNA magnetic isolation module (“NEB #E7490”). Sequencing of the libraries was performed using the NextSeq 500 with the NextSeq 500/550 High Output Kit v2 (75 cycles; Illumina).