NextSeq of Pseudomonas fluroescens SBW25: tRNA-del(gluTTC-glyGCC-gluTTC-glyGCC) compensated by serial transfer experimental evolution: evolved deletion mutant (M1-1)
The isolate was purified from an agar plate of the Day 21 population by streaking to purification on fresh KB agar. A single colony was grown up in KB culture and stored as a glycerol stock at -80C. A scraping of the glycerol stock was used to start an overnight culture in liquid KB (28C, shaking, ~16 hours). 1 ml of culture was used to isolate genomic DNA with the Qiagen Blood and Tissue extraction kit (following manufacturer's instructions). DNA quality was checked on an agarose gel and Nanodrop. The library was prepared for Illumina paired end NextSeq sequencing (2x250 bp).