GSM5570762: 960min_ABA(+) exp1 rep3; Populus nigra; RNA-Seq
2cm-long root apices were collected for all time points and immediately fixed in RNAlater (Sigma-Aldrich, St Quentin Fallavier, France). Total RNA was extracted from a pool of eight root apices collected at the same time-point using a Spectrum Plant Total RNA kit (Sigma-Aldrich, St Quentin Fallavier, France) complemented by a DNAse I treatment. RNA quality was assessed using a Fragment Analyzer 5200 (Agilent Technologies, Santa Clara, CA, USA). mRNA was purified using poly-(T) beads from 4 µg of each total RNA sample. RNA libraries were prepared at the Genotoul sequencing core facility (Toulouse, France) with TruSeq Stranded mRNA Sample Prep kit (Illumina, California, USA) according to manufacturer instructions