GSM5379942: EPI+SA+H_4; Mus musculus; RNA-Seq
RNA was extracted  with the ReliaPrep™ RNA Tissue Miniprep System (Promega, Z6111) following manufacturer's protocol for non-fibrous tissue by adding RNA lysis buffer on pelleted cells. Sequence libraries were prepared with the Lexogen QuantSeq 3' mRNA-Seq library prep kit according to the manufacturer protocol. Samples were indexed to allow for multiplexing. Library quality and size range were assessed using a Bioanalyzer (Agilent Technologies) with the DNA 1000 kit (Agilent Technologies, California, USA). Single-end reads of 50 bp length were produced with a minimum of 1M reads per sample