GSM6260863: WT 3; Mus musculus; RNA-Seq
BMDMs were isolated from Xbp1FL/FL and Xbp1M-KO mice and lysed in TRIzol reagent (Invitrogen, Carlsbad, CA) after LPS stimulation for 6 h, and then Xbp1FL/FL or Xbp1M-KO BMDMs RNA was harvested using Trizol reagent. NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (Cat#E7530L) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols