GSM5832428: CT_B; Escherichia coli; RNA-Seq
Bacterial cells were collected onto a 0.2 µm PES filter by low vacuum filtration. Two volumes of RNAprotect (Qiagen, Valencia, CA) were added to the cells, and the samples were then centrifuged at 5000 g for 25 min at 4 °C to pellet cells. RNA was isolated using the RNeasy Mini Kit (Qiagen, Valencia, CA) per the manufacturer's instructions. RNA libraries were prepared for sequencing according to Illumina's protocols using the Truseq Stranded mRNA Prep Kit.