GBS of Brassica napus: Young leaves
The genomic DNA of each sample was digested respectively with proper restriction enzymes based on the in silico evaluation results, and the obtained fragments were ligated with two barcoded adapters that either with a compatible sticky end with the primary digestion enzyme and the Illumina P5 or P7 universal sequence. Followed by several rounds PCR amplification, all the samples were pooled and size-selected for the required fragments to complete the library construction.