20h Sample of Metarhizium acridum
Total RNA was extracted from 15-d conidia of WT and mutants using Ultrapure RNA Kit and DNA was removed by RNase-free DNaseI. Samples were detected by BGISEQ-500. Prior to mapping to the M. acridum genome and annotated genes, raw data was filtered. In order to removed reads which is low quality, joint contamination or included with unknown base with a high content of nitrogen. The clean data was treated with randomness analysis, query coverage analysis, and saturation analysis before normalized to tags per million (TPM) to obtain normalized gene expression levels. Identification of differentially expressed genes was using a Q-value  0.001 and fold change 2. Differentially expressed genes were classified and annotated using gene ontology (GO) analysis.