Human Aligned RNA-Seq Data: Sample RS_58403195
"150ng of RNA was used as the input for first strand cDNA synthesis using Superscript III reverse transcriptase and Illumina's TruSeq Stranded mRNA Sample Prep Kit. Poly-A containing mRNA molecules were isolated using poly-T oligo attached magnetic beads. During the second elution of the poly-A RNA, the RNA was fragmented and primed for cDNA synthesis. Synthesis of the second strand of cDNA was followed by indexed adapter ligation. qPCR was performed on the cDNA libraries and were then normalized to 2nM. The TruSeq libraries were run on the Illumina HiSeq2500 platform with paired-end 2 x 101 bp reads with a targeted 50 million reads per sample by the Broad Institute. The raw sequences were aligned to the genome against the Gencode Genome Reference Consortium Build 38 (GRCh38.p12) and to the transcriptome against the Gencode Comprehensive Gene Annotation (GRCh38, v31) using the STAR aligner based on the TOPMed Genotype-Tissue Expression (GTEx) RNA-Seq analysis pipeline."