GSM5533894: NW074-E4; Homo sapiens; OTHER
Cells were crosslinked with freshly prepared 1% PFA and were then used for Nodewalk. The initial part of this technique is based on the 3C protocol with enzymatic digestion with Hind-III, and ligation using T4 DNA polymerase, generating ligated 3C DNA that is then used for Nodewalk library preparation. Nodewalk library preparation involves tagmentation using Illumina's transposome-linked beads from the Illumina® DNA Prep, (M) Tagmentation (cat.no. 20018704) kit. It is then followed by in vitro transcription for conversion to RNA, subsequent generation of cDNA and amplification of a specific region of interest (with interactors) using a specifically designed bait/primer. The final Nodewalk DNA is then sequenced using the Illumina MiSeq sequencing platform. Nodewalk-seq