NextSeq 500 sequencing; Transcriptome profiling of BT-20 cell line with C/EBPβ-ko
Transcriptome profiling of BT-20 cell line with C/EBPβ-ko
Triple-negative breast cancer cell line BT-20 were cultured in DMEM (Gibco) supplemented with 10% FBS, 10mM HEPES, 1mM Sodium Pyruvate and 100U/ml Penicillin Streptomycin. DMEM (Gibco) supplemented with 10% FBS, 10mM HEPES, 1mM Sodium Pyruvate and 100U/ml Penicillin Streptomycin RNA extracted with RNeasy Plus Mini Kit (QIAGEN) Library preparation was done using Illuminas TruSeq RNA v2 kit following the manufacturers description