GSM5381357: PC9_pgPEN_CRISPR_screen_LTP_RepC; Homo sapiens; OTHER
Genomic DNA was extracted using the QIAamp DNA Blood Maxi Kit (Qiagen catalog # 51192) with an additional overnight 4ºC incubation step before eluting the cleaned DNA from the column The paired gRNA sequences were amplfiied using a three-step PCR protocol as described in the Methods. Each sample was assigned a unique barcoded primer, and samples were diluted and pooled at equimolar ratios prior to sequencing