GSM5444682: Control_1_ (C57BL/6 x C3H) _PFC; Mus musculus; RNA-Seq
RNA extraction from PFC samples of biological duplicates in each group used the RNAeasy Mini kit (Qiagen), coupled to an RNase-free DNase step (Qiagen). The strand-specific RNA sequencing libraries were generated from 1 μg purified RNA using TruSeq stranded total RNA plus Ribo-zero kits (Illumina). The RNA sequencing libraries was constructed by TruSeq stranded total RNA plus Ribo-zero kits (Illumina). Sequencing was carried out with the HiSeq 2500 platform (Illumina) at the Genomics and Bioinformatics Core of State University of New York at Buffalo.Reads were first trimmed using Cutadapt to remove the 3' end adapters and trailing sequences, followed by aligning to mouse RefSeq mRNAs using TopHat2 with default parameters.