GSM5618662: Tumor in combined therapy group; Mus musculus; RNA-Seq
Tissue was processed to prepare scRNA-seq libraries by using Chromium Single Cell 3' Reagent v3 Kits (10x Genomics, Pleasanton, CA, USA). Single cells were suspended in calcium- and magnesium-free PBS containing 0.04% weight/ volume BSA. Then, single-cell suspensions were loaded onto a Chromium Single Cell Controller Instrument (10XGenomics, Pleasanton, CA, USA) to generate single-cell gel beads in emulsions (GEMs). After generation of GEMs, reverse transcription, cDNA PCR amplification and library preparation were performed accroding to the 10x Genomics 3' gene expression protocol.