ITS region amplicon sequencing of wheat associated soil metagenome
For Illumina ITS region amplicon sequencing, the amplicon library construction was done as described previously in Yergeau et al. (2015) based on a dual-indexing strategy that followed the 16S Metagenomic Sequencing Library preparation Illumina guide (Part #15 044 223 Rev. B). The V4 hypervariable region was amplified using the primers ITS1F and 58A2R (Martin & Rygiewicz, 2005). PCR products were purified, pooled and submitted for 2  250 bp Illumina MiSeq sequencing at the CESGQ.