GSM5618846: ∆dam∆metJ_SPI1inducing; Salmonella enterica subsp. enterica serovar Typhimurium; OTHER
DNA was extracted using a Dneasy Blood and Tissue Kit. The optional RNase step was performed. Multiplexed SMRTbell libraries for sequencing on the PacBio Sequel system were prepare from 1 μg of each microbial gDNA sample.  Shearing of gDNA was performed using g-TUBE and centrifugation at 2029 x g for 2 minutes to achieve a target mode size of 10 kb - 15 kb.SMRTbell libraries were then prepared using the SMRTbell® Express Template Prep Kit 2.0.  Two pools of 8 indexed libraries were prepared. Each pool was then sequenced on a PacBio Sequel SMRTcell using sequencing chemistry 3.0 and 10 hour movie length.