gH2AX ChIP-seq in HEK293T 00m after light-activation of cgRNA GG multi-target Cas9, delivered 12h ago | replicate 1, processed BAM file
ChIP using Rabbit gH2AX antibody conjugated to Protein A beads was performed on formaldehyde fixed cells; ChIP DNA was end repaired, A-tailed, ligated to sequencing adapters, and 10 rounds of PCR was performed