16S amplicon sequencing of synthetic human-gut microbiome: biological replicate 1
Amplicons were generated with Platinum Taq DNA Polymerase using 515F(Parada)/806R(Apprill) targeting the V4 region. Amplicons were amplified, purified and sequenced according to Illumina manufacturers recommendations (https://support.illumina.com/documents/documentation/chemistry_documentation/16s/16s-metagenomic-library-prep-guide-15044223-b.pdf)