RNAseq under romidepsin treatment in 3D7 parasites
For strand specific RNA sequencing upon mock vs romidepsin treatment, NF54 strain parasites were cultured in triplicates at 8% parasitemia and 2% hematocrit. Post double synchronisation, one set of replicates were treated with DMSO mock while the test set of replicates was treated with 360nM romidepsin (2XIC50) for 3 hours at 21 HPI and harvested at 24 hours post invasion. Post harvesting the parasites we proceeded with isolation of RNA from the TriZol suspended samples. The Thermo Colibri 3 mRNA library preparation kit was utilised for preparation of strand specific libraries. The final amplified libraries were quality checked on Agilent bioanalyzer for size distribution and Qubit fluorometer for quantity. Single end sequencing was done on the Illumina NextSeq 550 platform (150bp read length).