GSM4850348: mNPC_Cyto_rep 3_polyA; Mus musculus; RNA-Seq
Total RNA was collected with the Trizol LE regent for cytoplasmic RNAs, and Trizol reagent for soluble nucleoplasmic and chromatin-associated RNAs. Long RNA over 200 nt was selected using RNeasy MinElute Cleanup Kit (Qiagen). Polyadenylated RNA was selected using oligo-dT beads. Libraries were constructed with the Illumina TruSeq stranded kit. A final library of 250-400 bp was size selection with an agarose gel.