GSM5770374: Const-TIL; Homo sapiens; RNA-Seq
scRNA:  Tumors were harvested at Day 15 post tumor implantation. Tumors were mechanically dissociated using a gentleMACS dissociator (Miltenyi). Single-cell suspensions were made by passing tumors  through a 70μm cell strainer, depleting red blood cells by ACK lysis (Quality Biological Inc.), and further filtration through flow cytometry filter tubes with 35μm cell strainer caps (Falcon). T cells were purified by magnetic separation using the REAlease® CD4/CD8 (TIL) MicroBead Kit (Miltenyi) according to the manufacturer's instructions scRNA: Sent to the Stanford Functional Genomics facility for single-cell RNAseq library preparation using the Chromium Single Cell 3' Solution v3 platform (10x GENOMICS). Libraries were sent to Novogene for sequencing on a NovaSeq S4 lane (PE150) with >53,000 reads per cell