GSM5770448: 630; Bos indicus x Bos taurus; miRNA-Seq
A modified version of the Molecular Research Center Inc. (MRC; Cincinnati, OH, USA) TRI reagent RNA extraction protocol was used to extract total RNA from each of the frozen L. lumborum skeletal muscle biopsies (n = 78). During RNA extraction, each muscle sample was removed from the -80ºC freezer and placed within dry ice to prevent them from thawing. Approximately 100-200 mg of biopsied skeletal muscle tissue was used from each sample for RNA extraction and were all homogenized in liquid nitrogen using a ceramic mortar and pestle as described within the protocol. Sample libraries were prepared with the NEXTflex Small RNA-Seq kit v3 (BIOO SCIENTIFIC, Austin, TX) library kit. The quality of all sample libraries was evaluated via the Agilent 2200 TapeStation (Agilent Technologies, Santa Clara, CA) prior to sequencing, as recommended by the library preparation kit's protocol.