Metagenome from concrete road bridge
Sequencing libraries for the bridge sample and a negative control sample were prepared with the Illumina Nextera XT kit following the manufacturers instructions with several modifications (half reactions due to low input DNA, 12.5 minute tagmentation, 20 PCR cycles). Libraries were quantified with an AATI Fragment Analyzer (fragment length distribution ~150bp to 1600bp) and subsequently size selected using a Sage Scientific BluePippin, resulting in a final library with ~350 to 650bp fragments. Libraries were sequenced on an Illumina MiSeq with 150 bp paired-end reads.