Cecal microbiome of AGP treated broilers
DNA extraction and library preparation using the Shoreline Biome V4 16S DNA Purification and Library Prep Kit (Shoreline Biome, Farmington, CT). DNA was extracted from 10 microliters of homogenized fecal slurry. The V4 region of the 16S rRNA gene was PCR amplified using the 515F and 806R primers. Amplicons were sequenced using 2 x 250 bp paired-end kits on the Illumina MiSeq platform.