GSM5618816: Sample 2a [SKG Low Rep 2]; Mus musculus; RNA-Seq
Negatively selected CD4 T cells from the lymph node were sorted for CD62LhiCD44loCD25- and the 10% lowest (GFPlo) expressing T cells. Cells were washed, pelleted and immediately flash frozen using dry ice in ethyl alcohol. Sample was processed with Illumina TruSeq Stranded mRNA kit (Cat#: RS-122-2103) for library preparation. RNA libraries were prepared for sequencing using standard Illumina protocols