GSM6261565: PBS2; Mus musculus; RNA-Seq
Total RNA of whole lung was extracted using the RNAeasy™ Animal RNA Isolation Kit with Spin Column (Beyotime, Shanghai, China) according to the manufacturer's guidelines. mRNA was purified by magnetic beads with Oligo(dT), and then the purified mRNA was fragmented using fragmentation buffer at the appropriate temperature. The first-strand cDNA was then generated by PCR in the First Strand reaction system, and the same operation was performed for the second-strand cDNA. The PCR product was purified with magnetic beads and incubated with A-Tailing Mix and RNA Index Adaptors to complete the repair process. cDNA fragments with adapters were amplified using PCR and purified using Ampure XP Beads. An Agilent 2100 Bioanalyzer was used to assess the quality and quantity of the library, followed by DSN processing. Finally, it was sequenced and analyzed on the BGI DNBSEQ platform (BGI-Shenzhen, China).