16S rRNA gene amplicon sequencing of wheat associated soil metagenome
For Illumina 16S rRNA gene amplicon sequencing, the amplicon library construction was done as described previously in Yergeau et al. (2015) based on a dual-indexing strategy that followed the 16S Metagenomic Sequencing Library preparation Illumina guide (Part #15 044 223 Rev. B). The V4 hypervariable region was amplified using the universal primers 520F (5 - AGCAGCCGCGGTAAT- 3) and 799R2 (5 - CAGGGTATC TAATCCTGTT- 3) (Edwards et al. 2007), that exclude plant plastids 16S rRNA genes. PCR products were purified, pooled and submitted for 2  250 bp Illumina MiSeq sequencing at the CESGQ.