GSM5572681: shLONP1_FBXO11_2; Homo sapiens; RNA-Seq
CD34+ cells were lentivirally transduced to express the corresponding vector(s) and transduced cells were selected by expression of fluorescent reporter using FACs. Sorted cells were cultured in StemSpan with CC100 cytokine cocktail with 20ng/mL hTPO for 24 hours before TRIzol RNA extraction. 40ng of total RNA was submitted per sample for ribodepletion and library construction. Strand-specific ribodepleted RNA library construction. 150bp PE