GSM5065812: SGCAS Rep1 [RNA-seq]; Mus musculus; RNA-Seq
Total RNA was extracted from mESC, 2C::GFP+, 2C::GFP+/SGC and 2C::GFP+/SA cells using the Trizol kit (Thermo Fisher Scientific, catalog. no. 15596018) following the manufacturer's instructions. PolyA(+) RNA was isolated for library construction. RNA libraries were prepared for sequencing using standard Illumina protocols.