GSM5145221: WGBS GXGY; Homo sapiens; Bisulfite-Seq
Genomic DNA was extracted using the DNeasy Blood and Tissue Kit (Qiagen, Valencia, CA). Whole Genome Bisulfite conversions were performed with 200 ng of gDNA using the EZ DNA Methylation-Gold, 50rxn (Fisher-part#50444294) kit. WGBS Libraries were created using the Accel-NGS Methyl-Seq DNA Library Kit - 24 rxns (Swift Biosciences-part#30024) and Accel-NGS Methyl-Seq Dual Indexing Kit - 96rxns (Swift Biosciences-part#38096). The libraries were pooled and sequenced on 0.240 of an NovaSeq S4 flow cell (300 XP; targeting 30x wgbs coverage/120Gb per sample) at the Genome Technology Access Center at Washington University School of Medicine in St. Louis.