GSM5949459: mouse E12.5 submandibular glands_rep1; Mus musculus; RNA-Seq
Total RNA was extracted from mES derived iE-SGs at day 10 using RNeasy mini kit (Qiagen) following the manufacturer's instructions. RNA concentration and quality were measured by NanoDrop 2000 and the Agilent Bioanalyzer 2100 system. Sequencing libraries were prepared using NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.