GSM4979544: RNAseq YAP S127A overexpression at 48h (replicate 3); Homo sapiens; RNA-Seq
RNA was isolated from HUVECs using the miRNeasy micro Kit (Qiagen) combined with on-column DNase digestion (DNase-Free DNase Set, Qiagen) to avoid contamination by genomic DNA. RNA and library preparation integrity were verified with a LabChip Gx Touch 24 (Perkin Elmer). 4 μg of total RNA was used as input for TruSeq Stranded mRNA Library preparation following the low sample protocol (Illumina). Sequencing was performed on the NextSeq500 instrument (Illumina) using v2 chemistry, resulting in minimum of 32M reads per library with 1x75bp single end setup.