GSM5448129: PBMC from sample ACLF10; Homo sapiens; RNA-Seq
PBMCs and PMNs were isolated from 20 ml of EDTA blood. Blood samples were centrifuged at 200 g for 10 min to collect plasma and sedimented cells were diluted with DPBS -/- up to a volume of 20 ml. Diluted blood was layered over 13.3 ml of Ficoll-Hypaque and centrifuged at 500 g for 25 min with the break off. PBMCs were obtained from the mononuclear cell layer, washed with Dulbecco's PBS -/- and centrifuged again at 400 g for 5 min. Pelleted PMNs were incubated with