GSM5212432: runx1-/- kidney5 [runx1]; Danio rerio; RNA-Seq
Rnaseq on kidney: Wild type and runx1del8/del8 kidneys were dissected from ~2.5 months old fish ( pool of 3 kidneys/replicate) and immediately collected in Trizol (#15596018, Invitrogen). Samples were then put in a heatblock at 52C for 10 min and then overnight in -80C, RNA was extracted following the manufacturer protocol using Direct-zolTM RNA MiniPrep Kit (Zymo Research  #R2051). Sequencing libraries were prepaired using paired-end library preparation (TruSeq RNA Library Prep Kit v2)