GSM5448652: w1118_2; Drosophila melanogaster; RNA-Seq
Embryos were collected at 25°C (5-16 h after egg laying) and dechorionated in 2% sodium hypochlorite  solution for 4 min. After subsequent washing with embryo wash (0.8% NaCl and 0.05% Triton X-100) and H20, embryos were stored at -80°C. Once embryo collection for 3 biological replicates per genotype was obtained, RNA-extraction was carried out according to the manufacturer's protocol (Promega ReliaPrepTM RNA Tissue Miniprep System, REF-Z6111). Total RNA was measured using NanoDrop OneC (Thermo Scientific) for its concentration and RNA-integrity was checked on the gel electrophoresis. 9-15 µg of total RNA/biological replicate was shipped to Novogene Co. Ltd (UK) for sequencing. cDNA