GSM5494838: 20mix-LNP treated murine liver non parenchymal cells; Mus musculus; RNA-Seq
Cells were isolated 3 hours after injection with PBS or LNPs. Mice were perfused with 20 mL of 1X PBS through the right atrium. Liver tissues were finely minced, and then placed in a digestive enzyme solution with Collagenase Type I (Sigma Aldrich), Collagenase XI (Sigma Aldrich) and Hyaluronidase (Sigma Aldrich) at 37C at 550 rpm for 45 minutes. Cell suspension was filtered through 70μm mesh and red blood cells were lysed. Cells were stained to identify specific cell populations and sorted using the BD FacsFusion. Individually barcoded single-cell RNA-Seq libraries were prepared using the Chromium instrument and the Single Cell 3' Reagent kit (v3) following the manufacturer's protocol (10X Genomics). ~2000 cells were loaded for each of the three conditions. Library sequencing was performed on an Illumina Nextseq platform, using the 150-cycle High Output kit (Illumina) 10X Genomics 3' Gene Expression v3