RNAseq of S aureus exposed to human Urine
Total RNA was extracted from bacteria grown in human Urine using RibopureTM bacteria RNA extraction kit (Invitrogen). Library construction included DNase treatment (TURBO DNase, ThermoFisher Scientific) and rDNA depletion (QIAseq FastSelect, Qiagen) followed by RNA fragmentation and random priming. cDNA synthesis (NEBNext Ultra II, New England Biolabs) was followed by end repair, 5 phosphorylation and dA-tailing before. Libraries were sequenced on a partial lane of Illumina HiSeq 4000 with 150bp PE sequencing.