GSM6216283: hESCs_0_circFAT3_b5_rep5 [20200617_22_N286_10]; Homo sapiens; RNA-Seq
Total RNA was extracted with TRIzol Reagent (Invitrogen) following the manufacturer's guidelines. RNA quality was controlled using the RNA 6000 Nano Kit (Agilent Technologies, CA, USA) on a Bioanalyzer 2100 (Agilent Technologies), and RNA quantity determined using the Qubit RNA HS Assay Kit (Thermo Fisher Scientific) on a Qubit 4 Fluorometer (Thermo Fisher Scientific). The TURBO DNA-free Kit (Thermo Fisher Scientific) was used for DNA removal and followed by an additional RNA precipitation step.  For library generation, the QuantSeq 3'mRNA-Seq Library Kit (Lexogen, Vienna, Austria) was utilized according to the manufacturer's instructions.