GSM5870427: WT_whole_cell_circ_rep2; Mus musculus; ncRNA-Seq
The peripheral blood samples and TRizol reagent(Invitrogen, USA) are evenly mixed in a ratio of 1:3, total of 12 ml. Total RNA was extracted by using Trizol reagent according to the manufacturer's instructions. The RNA samples were utilized to construct whole transcriptome library by the TruSeq Ribo Profile Library Prep Kit (Illumina, United States), following manufacturer's instructions. For circRNA-sequencing, the RNase R digestion (10U, 1.5h) was conducted before library construction to enrich circRNAs.  The library products with 300-500 length were purified and quantified. These libraries were utilized to 150 nt paired-end sequencing with an Illumina Nova seq 6000 system (Novogene, China). Each library was generated a depth of ~8G basepairs