Whole-genome sequencing of wild-type E. coli
The library construction and sequencing were performed at the genomics facility at Instituto Gulbenkian de Ciencia (Portugal). Nextera XT kit from Illumina was used for library construction. Briefly, the wild-type E. coli was plated on LB agar and one clone was isolated. DNA was then extracted from this clone and used for whole genome sequencing in an Illumina NextSeq500.