GSM5597276: RNA-seq LSK- WT Rep2; Mus musculus; RNA-Seq
Total RNA from WT and XistΔ/Δ mouse bone marrow Lin- cells (~200,000) was extracted using TRIzol reagent (Invitrogen), and RNA from LSK+ or LSK- cells (~30,000-50,000) was extracted using RNeasy Plus Micro Kit (Qiagen, 74004). For Lin- samples, total RNA from 3 mice were combined per sample. For LSK+ or LSK- cells, total RNA from 2 mice were combined per sample. RNA-seq libraries of Lin- cells were prepared using Ovation RNA-Seq System Kit (NuGen, 0403-32 and 0348-32), and LSK+ or LSK- RNA-seq libraries were prepared using SMART-Seq v4 Ultra Low Input RNA Kit (Takara, 634888) and KAPA HyperPrep kit (KAPA, 07962347001).