16s metagenomics sequence of fermentation of food waste with bacteria agent "T2"_replicate-1
The genomic DNA of the food waste fermentation product was extracted using HiPure Stool DNA kit (model D3141, Magen Biotechnology Co., Ltd, China) according to the manufacturer's guidelines. 1% agarose gel electrophoresis was used to detect whether the DNA was successfully extracted. A NanoDrop spectrophotometer (Model NanoDrop 2000, Thermo Fisher Scientific, USA) was used to determine the DNA quality (Wanget et al., 2020). Using barcode-specific primers 341F (CCTACGGGNGGCWGCAG) and 806R (GGACTACHVGGGTATCTAAT) to amplify the 16S rDNA V3-V4 hypervariable region (Zhang et al., 2021). The PCR product was purified using AMPure XP Beads, and quantified with Qubit3.0 after purification. Based on the PE250 mode of Novaseq6000 for pooling on-machine sequencing (Guangzhou Gideo Biotechnology Co., Ltd., Guangzhou, China).