GSM5831996: Thymus, Cd226KO; Mus musculus; RNA-Seq
Tissues were digested in GEXSCOPE Tissue Dissociation Solution (Singleron Biotechnologies, Nanjing, China) and were passed through 40-μm nylon sterile strainer to obtain single-cell suspensions. Single-cell suspensions with 1 × 10^5 cells/ml in PBS (HyClone) were prepared. The suspensions were then loaded onto microfluidic devices. scRNA-seq libraries were constructed according to the Singleron GEXSCOPETM protocol using the GEXSCOPE™ Single-Cell RNA Library Kit (Singleron,1110011 )