WGS of Franconibacter sp. isolated from oil contaminated soil
The paired-end sequencing library was prepared from the QC passed genomic DNA samples using illumina TruSeq Nano DNA Library Prep Kit. Briefly, approximately 200 ng of DNA was fragmented by Covaris M220 to generate a mean fragment distribution of 350bp. Covaris shearing generates dsDNA fragments with 3' or 5' overhangs. The fragments were then subjected to end-repair. This process converts the overhangs resulting from fragmentation into blunt ends using End Repair Mix. The 3' to 5' exonuclease activity of this mix removes the 3' overhangs and the 5' to 3' polymerase activity fills in the 5' overhangs followed by adapter ligation to the fragments. This strategy ensures a low rate of chimera (concatenated template) formation. The ligated products were size selected using AMPure XP beads. The size-selected products were PCR amplified with the index primer as described in the kit protocol indexing adapters were ligated to the ends of the DNA fragments, preparing them for hybridization onto a flow cell. The PCR enriched library was analyzed on 4200 Tape Station system (Agilent Technologies) using high sensitivity D1000 Screen tape as per manufacturer instructions.