GSM6195667: Monocytes, MCD-fed, normal saline gavaged, rep2; Mus musculus; RNA-Seq
Eight-week-old weight-matched male wild-type (WT) C57BL/6 mice and Rag1-/- mice were purchased from The Jackson Laboratory (Bar Harbor, ME, USA). These mice were fed either a normal control diet (NCD), a methionine/choline-deficient diet (MCD, 10401, Beijing HFK Bioscience, Beijing, China), or an MCD diet with melatonin (100g/kg per day) treatment by oral gavage for six weeks. The immune cells were harvested, and then the expression levels of various cell surface and intracellular markers were detected. All samples were examined through a FACS Aria II flow cytometer (BD Biosciences, CA, USA), and the data were analyzed using FlowJo software (Treestar, Ashland, OR, USA). Library was performed according to the manufacter's instructions (single cell 3' v3 protocol, 10x Genomics).