GSM4850318: ZIKV_miRNA_H41R; Homo sapiens; miRNA-Seq
For In Vivo miRNA-seq, 1x106 NSCs were infected with ZIKV-WT or ZIKV-H41R at MOI of 1 and total RNA was extracted with at 2 dpi. miRNA was isolated for construction of miRNA sequencing library with QIAseq miRNA Library Kit (Qiagen, #331505) by UCLA Technology Center for Genomics & Bioinformatics (TCGB), followed by deep sequenced using Illumina HiSeq3000. Briefly, the workflow consists of 3' ligation, primer hybridization, 5' ligation, first strand synthesis and PCR amplification. Different adapters were used for multiplexing samples in one lane. AMPure XP Beads were used for dual size selection. Library quality was checked with Bioanalyzer (Agilent) and Qubit (Life Technologies).