GSM5065530: CD28_CD3_DIG_control_Multiome_ATAC; Homo sapiens; ATAC-seq
Control and stimulated PBMCs were stained with a TotalSeqA antibody panel (210 antibodies), sorted to remove dead cells and granulocytes, and permeabilised with two methods: 1) 0.1% PFA fixation followed by permeabilization with LLL buffer and 2) permeabilization with 0.1% digitonin . Cells were subsequently subjected to transposition and loaded into the 10x Multiome chip for the barcoding reaction. ATAC and GEX libraries were constructed according to the 10x Genomics single cell Multiome protocol. Protein tags were captured via the polyT barcoded oligos and amplfied using tag-specific handles. 10x Genomics Multiome + CITE-seq antibody capture