GSM6261193: HES-2, LN, rep0 [RRBS]; Homo sapiens; Bisulfite-Seq
hPSCs were detached from the matrix or MEFs by treatment with Collagenase type IV. Genomic DNA was extracted. RRBS library was prepared as described (Boyle et al., 2012). Bisulfite conversion of RRBS library was performed using the EpiTect DNA Bisulfite Kit (Qiagen) following manufacturer's protocols.