GSM5993823: MEF-0hr-2; Mus musculus; RNA-Seq
For library construction, DNA/RNA is extracted from a sample. After performing quality control (QC), qualified samples proceed to library construction. The sequencing library is prepared by random fragmentation of the DNA or cDNA sample, followed by 5' and 3' adapter ligation. Alternatively, "tagmentation" combines the fragmentation and ligation reactions into a single step that greatly increases the efficiency of the library preparation process. Adapter-ligated fragments are then PCR amplified and gel purified.