GSM5333271: GBA 3-weeks old rep1; Drosophila melanogaster; RNA-Seq
Flies were flash frozen in liquid nitrogen, and decapitated on dry ice. RNA was harvested using Trizol reagent. Samples were polyA enriched using the Kapa mRNA HyperPrep kit. Illumina NextSeq v2.5, 150 cycles was used with 120ng of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols