GSM5381306: Cre-only_Control Replicate 2; Mus musculus; RNA-Seq
Individual pancreata were dissected and placed in RNA TM later (Invitrogen) overnight at 4 C and subsequently at -80 C until ready to process. Total RNA was extracted using TRIzol reagent (AMBION) and PureLinkTM RNA Mini Kit (Invitrogen). RNA libraries were prepared for sequencing using standard Illumina protocols