GSM6259961: Challenged Vegfr3iLECko rep2; Mus musculus; RNA-Seq
Total RNA was extracted from freshly dissected conjunctiva using the Qiagen RNeasy Plus Kit. To obtain sufficient quantitation of RNA, conjunctival tissue from three mice with identical genotyping and treatment condition was combined and used as one sequencing sample, and a total of three relicate experiments (3x3mice in each group) were performed. RNA quality was evaluated via Agilent 2100 Bioanalyzer. One microgram of qualified RNA was used to construct mRNA sequencing libraries.