GSM4851042: P0_GFP_High; Mus musculus; RNA-Seq
Digestion by 1.25% pancreatin and 0.125% Trypsin EDTA followed by Collagenase II/Elastase (both 1 m/mL) digestion.  Cells were passed through a 30 um mesh filter prior to FACS where GFP-high and GFP-low cells were collected.  ~100,000 cells per population were collected. RNA was extracted using Arcturus PicoPure RNA Isolation kit. Stranded libraries were constructed using Illumina Truseq mRNA kit and sequenced using an Illumina HiSEQ4000 (1x50bp) to a depth of ~75M reads/sample.