GSM5448666: ckit_pos.1; Mus musculus; RNA-Seq
Cells were isolated by rapid mechanical disaggregation of TDLNs from established (day 14) untreated B16F10 tumors.  LNs were pooled and stained for CD11c, Ly6c and c kit, then the CD11cNEGLy6c+c kit+ precursor population was sorted and used for RNA-seq.Total RNA was isolated using TRIzol reagent. The RNA-seq libraries were constructed using 8 ng of total RNA with SMARTer Stranded Total RNA-seq kit v2-Pico Input library preparation kit (TaKaRa Bio).