GSM5065783: STL_S2_ts_3m_2; Drosophila melanogaster; RNA-Seq
RNA was harvested with Trizol reagent, followed by chloroform extraction and isopropanol precipitation. For transient-transciptome-TimeLapse-seq (TT-TL-seq) genomic DNA was depleted using Turbo DNAse, followed by cleanup with RNAclean beads. RNA for transient-transciptome-TimeLapse-seq RNA was enriched with MTS-biotin and treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018. For Start-TimeLapse-seq, total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018 using mCPBA as the oxidant and short capped RNA was enriched as described in Nechaev et al. Science 2010. TT-TL-seq libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian, V2 (Takara Bio USA, cat. 634413). STL-seq libraries were generated with custom oligos following methods described in Nechaev et al. Science 2010.