RNAseq of 3 day old M. truncatula seedling roots treated with the solvent control - DMSO (pH adjusted to 6.8). Each replicate comprises 40-60 seedling roots.
RNA-seq libraries were obtained from roots of Medicago truncatula A17 plants three days post germination. The seedlings were immersed in water (pH 6.8) containing the hormones at different concentrations; 1 uM IAA (Auxin), 100 nM 6-BAP (Cytokinin) and an equal volume of the solvent control (DMSO). The seedlings were placed in the dark for 3 hours after which the root tips were removed and 40-60 roots per replicate were used.