Multiple New Species within the genus Pseudomonas
Illumina compatible Genomic DNA libraries were generated on using Kapa Biosystems Hyperplus library preparation kit (KK8514). DNA was enzymatically sheared to approximately 500bp fragments, end repaired and A-tailed as described in the Kapa protocol. Illumina-compatible adapters with unique indexes (IDT #00989130v2) were ligated on each sample individually. The adapter ligated molecules were cleaned using Kapa pure beads (Kapa Biosciences, KK8002), and amplified with Kapas HIFI enzyme (KK2502). Each library was then analyzed for fragment size on an Agilents Tapestation, and quantified by qPCR (KAPA Library Quantification Kit, KK4835) on Thermo Fisher Scientifics Quantstudio 5 before multiplex pooling and sequencing a 2x250 flow cell on the MiSeq platform (Illumina) at the ASUs Genomics Core facility.