RNA-Seq of the diploid hybrid of Oryza sativa  Oryza punctataC
Subsequently, size fractionation and selection (16 kb) were conducted using the BluePippin Size-Selection System (Sage Science, Beverly, MA). A Single Molecule, Real-Time (SMRT) bell library was constructed with the PacBio DNA Template Prep Kit 2.0. The library was subsequently sequenced on the PacBio RS II platform with P6C4 polymerase enzyme and chemistry.