GSM4849240: LGL141; Homo sapiens; miRNA-Seq
T-LGL and healthy control CD8 TEMRA cells were lysed in Trizol (Invitrogen) and total RNA was isolated according to manufacturer's protocol. Quality and concentration of total RNA of the samples was determined with the RNA 6000 Nano assay on an Aligent 2100 Bioanalyzer (Agilent Technologies). The small non-coding RNA cDNA libraries were generated with 1 ng total RNA using CleanTag Small RNA Library Preparation Kit (Trilink Biotechnologies) according to the manufacturer's protocol.