Deep-Seq of mus musculus: adult cochlea
The on-target region within the genomic DNA extracted from mouse cochlea tissue samples were amplified using eTaq or Pfu DNA polymerase (Promega, Madison, WI). Equal amounts of the PCR amplicons were subjected to paired-end read sequencing using Illumina MiSeq at Bio Medical Laboratories. The region including the target sites were amplified by nested PCR using appropriate primers.