GSM4977975: NT_outgrowth.rrbs.rep4; Mus musculus; Bisulfite-Seq
For the RRBS library, we digested nuclear proteins and extracted the DNA from the nuclei of samples. Then, we add unmethylated lambda DNA (Fermentas) and took a one-tube reaction, treating the DNA with MspI digestion (Fermentas, USA), end repair, dA tailing, adaptor ligation, and bisulfite conversion using MethylCode Bisulfite Conversion Kit (Invitrogen, USA, MECOV-50). We purified the converted libraries by Agencourt AMPure XP beads (Beckman A63881, USA) and amplified using two-round PCR enrichment. Only 200–500 bp DNA fragments were reserved for sequencing.