GSM5331299: Dhcr24TG XZ14; Mus musculus; RNA-Seq
Mice were injected intraperitoneally with 3% thioglycollate broth to elicit peritoneal macrophages after 16 weeks on CD or WD diet. Thioglycollate-elicited macrophages were allowed to attach on 6-well plates for 2 hrs and washed twice with PBS to remove unattached cells. The cells were collected immediately after PBS wash for CD/WD group or cultured in 10% lipoprotein-deficient serum for 48 hrs for resting macrophage group. Total RNA was extracted and purified using a RNA isolation Kit (Qiagen) followed by DNAse treatment to remove genomic contamination using RNA MinElute Cleanup (Qiagen). rRNA was depleted from RNA samples using Ribo-Zero rRNA Removal Kit (Illumina). RNA libraries from WT BMDMs were performed TrueSeq Small RNA Library preparation (Illumina). RNA libraries were sequenced for 45 cycles on Illumina HiSeq 2000 platform (1 x 75bp read length).