Salivary glands from O. triseriatus male sample 3
Intact salivary glands pairs were collected from sugar-fed adults (1- to 3-days old) and pooled together in 100 ul of TRIzol (Invitrogen). Three biological samples (pools) containing 50 salivary glands pairs (females) or 100 pairs (males) were collected. The total RNA was isolated using TRIzol (Invitrogen) according to the manufacturer instructions and analyzed with an Angilent 2100 Bioanalyzer system (Agilent). Sequencing libraries were generated using NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB) following manufacturers and sequecing was performed in a Illumina Novaseq 6000 DNA sequencer.