WGS of Pseudomonas aeruginosa: Cystic Fibrosis isolate
Isolates were collected from a patient lung using bronchoalveolar lavage (BAL). During the BAL procedures, the right lung was sampled in the order of upper lobe (RUL), middle lobe (RML), then lower lobe (RLL). Immediately after sample collection, BAL fluid was spread directly onto Pseudomonas isolation agar and blood agar plates with a metal spreading tool and incubated at 37C for 48 h. Individual colonies were picked, patched onto PIA, and grown for 36-48 h. Single colonies were  grown in 96 well plates at 37C in 300 ul of LB. Beckman GenFind V2 kits were used to extract genomic DNA, which was subsequently normalized using the Qubit dsDNA BR Assay kit. Nextera XT kits are used to construct libraries consisting of roughly 500 bp DNA size selected fragments and were normalized with the Qubit dsDNA HS Assay kit. Bioanalyzer analysis was submitted to the Roy J. Carver Technology Center at the University of Illinois Urbana-Champaign. Libraries were sequenced with Illumina HiSeq2500 platform at the Roy J. Carver Technology Center to obtain 250nt paired end reads.