Sequel II sequencing
PacBio HiFi sequencing for genome assembly.
A HiFi library was prepared according to the manual Procedure & Checklist - Preparing HiFi SMRTbellÂ® Libraries using SMRTbell Express Template Prep Kit 2.0 with an initial DNA fragmentation by g-Tubes (Covaris) and final library size binning into defined fractions by SageELF (Sage Science). Size distribution was again controlled by FEMTOpulse (Agilent). Size-selected library with an expected insert size of 15 kbp was independently sequenced on a single SMRT cell of a Pacific Biosciences Sequel II device at the MPGC Cologne with Binding kit 2.0 and Sequel II Sequencing Kit 2.0 for 30 h (Pacific Biosciences).