PacBio HiFi sequencing of Scomber colias : adult muscle
A portion of muscle the muscle was snap frozen in liquid nitrogen. The gDNA with high molecular weight was extracted from muscle using QIAGEN Genomic-tip 20/G Kit. The quality and concentration of gDNA was assessed with Qubit Fluorometric system (ThermoFisher), while the fragment size was determined with Fragment analyser (Agilent) prior to loading on the Sequel II. The Hifi sequencing library was performed following the manufacturers recommendations (Pacific Biosciences) (https://www.pacb.com/wp-content/uploads/Procedure-Checklist-Preparing-HiFi-SMRTbell-Libraries-using-SMRTbell-Express-Template-Prep-Kit-2.0.pdf) in BYU. The size-selected fraction had 15.3 kb mean read length and was selected on Sage-Elf system (Sage Sciences). The sequencing was conducted on 2 SMRT cells using Sequel II system (v9.0), with run time of 30h and 2.9 hour pre-extension. The circular consensus analysis was performed in SMRT Link v9.0 under the default settings.