GSM5494274: CRC-35M; Homo sapiens; RNA-Seq
Human CRC spheroid cells (1x10^5), cultured in Spheroid buffer, were collected by centrifugation at 500 xg for 1min at 4C. Cells were then washed twice with PBS(-) and total RNAs were purified with RNAeasy kit (Qiagen) according to the manufacturers instruction. Indexed cDNA libraries were generated from each 500 ng of the purified RNA with TruSeq stranded mRNA kit (Illumina) RNA libraries were prepared for sequencing using standard Illumina protocols