GSM5212829: wound healing tissue single cell RNA seq; Mus musculus; RNA-Seq
After resuspension in 1× PBS, all single-cell suspensions were loaded into a 10x Chromium Controller (10x Genomics, USA). Barcoded sequencing libraries were generated using Chromium Single Cell 3' Library & Gel Bead kits v2 (cat# PN-120237, 10x Genomics, USA) and sequenced on a Hiseq 2500 platform (Illumina, USA). Chromium Single Cell 3' Library & Gel Bead Kit v2 (cat# PN-120237) Chromium Single Cell A Chip Kit (cat# PN-120236) Chromium i7 Multiplex Kit (cat# PN-120262) Following the manufacturer's instructions, barcoded sequencing libraries were generated using Chromium Single Cell 3' v2 kits. Sequencing was performed on Illumina Hiseq2500