GSM4978400: Input_rep1; Homo sapiens; ChIP-Seq
Crosslink of proteins-chromatin was performed with formaldehyde solution. Crosslink reaction was stopped with glycine. Cells were lysated and sonicated for 7 cycles of 30'' on 30'' off in a Bioruptor Sonicator. Chromating was co-immunoprecipitated using anti-HA antibody (abcam, AB9110). Libraries were prepared according to Illumina's instructions accompanying and were sequenced on the Illumina HiSeq 2500 following the manufacturer's protocols.