GSM6044072: CRC_primary_1; Homo sapiens; ATAC-seq
Nuclei Isolation and all procedures were performed as described in the manufacturer's protocol (Nuclei Isolation for Single-Cell ATAC-Sequencing, CG000168 Chromium Single-Cell ATAC Reagent Kits User Guide ,10X Genomics). Following the 10x Genomics single cell ATAC solution, by using Chromium Chip E Single Cell Kit(Product Code 1000086 ) and Chromium Single Cell ATAC Library & Gel Bead Kit (Product Code 1000110), the nucleus in bulk sample are partitioned into nanoliter-scale Gel Beads-in- emulsion (GEMs), a pool of ~750,000 10x Barcodes is sampled to separately and uniquely index the transposed DNA of each individual nucleus, and libraries are generated(performed by CapitalBio Technology, Beijing). The libraries was sequenced using an Illumina Novaseq sequencer with a sequencing depth of at least 25k read pairs per nucleus.