GSM5570632: S19a_18189 Run2; Homo sapiens; ATAC-seq
Nuclei were extracted according to the 10X Genomics demonstrated protocol for frozen tissue (CG000212 Rev B), modified to include an additional wash with Wash buffer. Tissue homogenization was performed using a dounce homogenizer. Filtered nuclei suspension was  pelleted as instructed and then resuspended in 50 ul Storage Buffer from the EZ nuclei kit (Sigma Aldrich, NUC101). For downstream processing, nuclei were thawed briefly on ice, resuspended with 500 ul Wash Buffer and gently pipetted.  Nuclei were pelleted at 500 × g (5 min, 4 ˚C) then resuspended again in 500 ul Wash Buffer before counting and continuing to Chromium capture. scATAC-seq libraries were generated according to the Chromium Single Cell ATAC protocol (10 × GENOMICS, CG000168) as described previously (Satpathy et al. 2019) and sequenced on the Illumina NextSeq 500 platform.