DNA-Seq of female moth
The DNA sample was fragmented by sonication to produce 350-bp fragments, then these DNA fragments were end-polished, A-tailed, and ligated with the full-length adaptor for Illumina sequencing with further PCR amplification. Finally, PCR products were purified using the AMPure XP system. The libraries were analyzed to determine size distribution with an Agilent2100 Bioanalyzer and quantified using real-time PCR.