Metagenomic sequencing of chimpanzee fecal sample K-OM-16-Q4
We homogenized fecal samples, treated the homogenate with nucleases to reduce unencapsidated nucleic acids, and performed subsequent extractions with the Qiagen QIamp MinElute Virus Spin Kit (Qiagen, Hilden, Germany). We synthesized cDNA from the extracted RNA, purified cDNA using Agencourt AmpureXP beads (Beckman Coulter, Brea, CA, USA), and prepared libraries using the Illumina Nextera XT kit (Illumina, San Diego, CA, USA).