RNA-Seq of Control iPSC derived neuron rep2
Total RNA was extracted from the iPSC-derived ASD neurons and control neurons. The quality and integrity of the extracted total RNA were assessed using BioAnalyzer and the standard Illumina sequencing system protocol (TruSeq RNA sample preparation kit) was used to make libraries for RNA-Seq. Approximately 300-bp-long fragments were isolated using gel electrophoresis; they were then amplified by PCR and sequenced.