GSM5494294: JV_H3K27me3; Mus musculus; ChIP-Seq
Murine BM nucleated cells (BMNCs) were isolated by flushing the femur and tibia into PBS with 2% Fetal bovine serum (FBS, Gibco) and 2 mM EDTA (Invitrogen), lysed with red blood cell (RBC) lysis buffer (Solarbio) for 15 min, and then centrifuged at 4°C. BMNCs was enriched for c-kit using CD117 MicroBeads (Miltenyi, 130-091-224) and separated using an AutoMACS Pro separator (Miltenyi) according to the manufacturer's specifications. Libraries were prepared for sequencing using standard Illumina protocols