Illumina 16S amplicon sequence of bacteria in maple seedling roots
a portion of the small subunit (SSU) RNA gene was amplified using the Glomeromycota specific primer AML2  and universal eukaryotic primer WANDA .  All amplified PCR samples were cleaned and normalized using the Invitrogen Sequalprep PCR Cleanup and Normalization Kit. DNA library was sequenced using an Illumina MiSeq Sequencer with the 2 300 bp paired end platform using the Illunima MiSeq Reagent Kit v3.