Gleditsia fera (Lour.) Merr.
The genomic DNA was extracted by using a modified CTAB method, and the purity and integrity of the DNA were analyzed by agarose gel electrophoresis. The purity and integrity of the DNA were tested by Nanodrop. After the DNA samples were qualified, they were interrupted by the Covaris ultrasonic disruptor. After end repair, A-tailing, sequencing adapters, purification, PCR amplification and other steps were applied to completely construct the library. After the library is qualified, the Illumina high-throughput sequencing platform NovaSeq 6000 is used for sequencing.