GSM5813683: GBM3; Mus musculus; RNA-Seq
Ribotag ip RNA extracted from immunoprecipitated polyribosomes was used to prepare libraries by NebNext rRNA depletion kit (#E6310), NEBNext® Ultra™ II RNA Library Prep Kit for Illumina (#E7770G), and NEBNext® Multiplex Oligos for Illumina® (#E7335G) kit, according to manufacturers' instructions. Libraries were normalized, pooled, and sequenced on the Illumina NextSEQ 500 with the NextSeq 500/550 Mid Output Kit v2.5 (150 Cycles) (#20024904), according to manufacturers' instructions