Nanopore sequencing of Posion Lake anoxic water
Poison lake anoxic water was collected by boat using a peristaltic pump (2L). A 50 ml subsample was frozen until further processing. In the laboratory, sediment was subsampled for DNA extraction with a Qiagen Power Soil kit (Qiagen, USA). Metagenomic DNA sequencing library was prepared using ONT Sequencing Ligation Kit (SQK-LSK110) as described by manufacturer. Library was sequenced on a r9.4.1 MinION Flowcell