RNA-Seq of Reticulitermes chinensis third instar workers infected with Serratia marcescens
The messenger RNA (mRNA) was isolated from total RNA by the poly-T magnetic bead method. First-strand cDNA was synthesized with random hexamers, and the sec-ond-strand cDNA was synthesized with DNA polymerase I, RNase H and dNTPs. The cDNA library was sequenced using the Illumina NovaSeq platform.