GSM4851100: Microglia_P15_WT_rep1; Mus musculus; RNA-Seq
mice were transcardially perfused using ice-cold Hank's balanced salt solution (1X HBSS), S1 was dissected and a single cell suspension was generated, the suspension was enriced for microglia using Percoll gradient centrifugation, microglia were then blocked, stained for Cd11b-PE, CD45-APC-Cy7 and Cx3cr1-APC and purified from the suspension by fluorescence-activated cell sorting (FACS) using a 100 μm chip on a SH800S Sony sorter. Single-cell RNA-seq libraries were prepared on the 10x Genomics platform using the Chromium Single Cell 3' Library and Gel Bead Kit v3.0 (PN-1000075), Chromium Single Cell 3' Library Construction Kit v3.0 (PN-1000078), Chromium Chip B Single Cell kit (PN-1000154) and Chromium i7 Multiplex Kit (PN-120262) as instructed by the manufacturer. Libraries were sequenced using the Nova-Seq 100 cycle kit (Illumina) by Broad Institute Genomic Services