GSM5444675: tsh1_rep3; Zea mays; RNA-Seq
ear primordia were fixed overnight in 3.5% paraformaldehyde, dehydrated through an ethanol series, transferred to Histoclear before embedding in paraffin. Embedded samples were sectioned at 5 µm and mounted onto charged HistoBond slides . The slides were then subjected to laser microdissection by using a PALM microbeam system. Three biological replicates were prepared per genotype (B73, tsh1, tsh4, and tsh1 tsh4). Approximately 750 microns of cells were harvested for each biological replicate. RNA was extracted from microdissected tissues with the PicoPure RNA Isolation Kit and in vitro amplified using a TargetAmp 2-round aRNA Amplification Kit 2.0. RNA-seq libraries were constructed using a TruSeq Stranded mRNA Library Prep Kit and quantified on an Agilent bioanalyzer (Agilent)