Targeted capture of Symhpnoia globulifera: P1-1-2880
We designed in silico 20,000 80-mer probes for sequence capture on Symphonia globulifera. The genetic resources used for the design consisted of two draft genomes, a transcriptome, and reduced-representation genomic sequence reads. We prepared and pooled libraries for each individual including a few replicates, captured sequences by hybridization with probes, and sequenced them in two lanes of an Illumina HiSeq 4000 instrument, following standard protocols