GSM6044896: WT_Uninjured 4; Mus musculus; RNA-Seq
Control and T1DM mice were euthanized at 16 weeks old. Knee joints were collected, dissected and cut at the edges of the joint region with small traces of soft tissue to preserve the articular integrity. Joints were then homogenized in Qiazol (79306, Qiagen, Valencia, CA, USA) using a PRO tissue homogenizer (Bio-Gen PRO 200, PRO Scientific Inc.). Total RNA was purified using RNeasy Mini Kit (QIAGEN Inc., Germantown, MD, USA) according to the manufacturer's protocol and the RNA integrity was assessed using a bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Poly(A)+-enriched cDNA libraries were generated using the Illumina TruSeq RNA Library Prep kit v2 (Illumina Inc., Hayward, CA, USA).