GSM5570295: targeted_nanoNOMe_CD4rest_0h_rep1; Homo sapiens; OTHER
Nuclei were isolated and subjected in situ to a GpC methylation labeling reaction (NEB, M.CviPI). HMW gDNA was extracted using AMPure XP beads (Beckman Coulter) after proteinase K and RNase A treatments. Purified DNA was dA-tailed using the NEBNext dA-Tailing Module (NEB) and subjected to nanopore adapter ligation (ONT, SQK-LSK109)