GSM6194036: prEpiSCs_ATAC_seq_1; Homo sapiens; ATAC-seq
A total of 50,000 cells were resuspended in 50 µl lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% (v/v) NP-40). The nuclei were collected by centrifuged for 10 minutes at 500g at 4°C  and then mixed with the transposition reaction mix (10 µl TTBL, 5 µl TTE Mix V50 and nuclease-free H2O up to 50 µl in total) of TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme, catalog. no. TD501) for 10 minutes at 55 °C. DNA Samples were then PCR amplified. PCR products were mixed with 1.2 volume of DNA clean beads to clean up. Library integrity was checked by the Agilent bioanalyzer. The libraries were sequenced on an Illumina Hiseq X10 platform and 150 bp paired-end reads were generated.