GSM5284887: B6IgE2; Mus musculus; RNA-Seq
Total RNAs were extracted from BMCMCs using Rneasy Micro kit (Quiagen, Venlo, The Netherlands). Briefly, mRNA from Eukaryote organisms is purified from total RNA using poly-T oligo-attached magnetic beads (For prokaryotes, mRNA was purified through the removal of rRNA by using kit). The mRNA is first fragmented randomly by addition of fragmentation buffer. According to demand of customer, we have NEB library and strand specific library to choose.