NextSeq 500 paired end sequencing of SAMD00241733
To remove fragmented DNA from dead cells, cultured cells were incubated with DNase solution (200 units/mL) at 37 degree for 30 min. After replacement of media, adherent cells were washed with PBS 3 times and enzymatically detached using trypsin. 100,000 cells per sample were collected and gently freezed within a pre-chilled freezing container filled with 100% isopropyl alcohol at -80 degree to complete cryopreservation. The thawed cells were lysed with lysis buffers and then the nuclei were processed for the tagmentation reaction. After reaction, tagmented DNA was purified and PCR-amplified. The DNA libraries were then sequenced using Illumina sequencer.