S2_17
Genomic DNA from cell lines was extracted using the (insert your extraction technique here) and screened with the Agilent TapeStation (Agilent Technologies, Inc., Wilmington DE). Whole-exome sequencing was performed using the Agilent SureSelect XT Clinical Research Exome kit (Agilent Technologies, Inc., Wilmington DE). Briefly, 200 ng of DNA was used as input to construct libraries following the manufacturers protocol. Libraries were evaluated using the Agilent BioAnalyzer (Agilent Technologies, Inc., Wilmington DE), and equimolar amounts of library DNA were used for whole-exome enrichment using the Agilent capture baits. The final libraries were assessed for quality on the Agilent BioAnalyzer, and quantitative PCR for library quantification was performed using the Kapa Library Quantification Kit (Roche Sequencing, Pleasanton, CA). The libraries were sequenced on the Illumina NextSeq 500 v2 sequencer with two 75-base paired-end runs in order to generate 100-120 million read pairs per sample aiming for 100X target coverage.