GSM5494475: HNSCC201125T04; Homo sapiens; RNA-Seq
Patient tumors were fresh frozen and sectioned at 10um thickness onto spatial transcriptomics (ST) slides for mRNA capture, followed by library preparation. Libraries were prepared using a Chromium Single Cell 3' Reagent Kit (10X Genomics, v3 kit) following the directions of the manufacturer. RNA libraries were prepared for sequencing using standard Illumina protocols. RNA libraries were prepared for sequencing using standard Illumina protocols 10X Visium platform