Whole-genome sequence of Rhizobium anhuiense strain:T24 using ONT long reads and Illumina short reads
For Nanopore sequencing, libraries were prepared using the Ligation Sequencing Kit 1D (SQK-LSK109, ONT) and EXP-NBD104 native barcoding expansion according to standard protocols with the following modifications. Genomic DNA was subjected to End Repair and A tailing by NEBNext FFPE DNA Repair mix and NEBNext Ultra II End repair/dAtailing module (NEB) prior to the ligation. Sequencing adaptors were ligated using NEBNext Ultra II DNA Ligation module (NEB) after AMPure XP (Beckman Coulter) magnetic beads purification step. The final product was cleaned up using Long Fragment Buffer. MinION sequencing was performed as per manufacturers guidelines using R9.4 flow cells (FLO-MIN106D, ONT). Reads were created based on FAST5 files by Guppy 6.0.1 [8] using the "sup" ("super accuracy") mode.