GSM5742813: GR07; Canis lupus familiaris; RNA-Seq
RNA was isolated from RNAlater stored blood with the RiboPure™-Blood Kit (Ambion, Life Technologies, CA, USA). From each sample 1 ug of high quality RNA (RNA integrity number (RIN) > 8) was used for non-stranded, paired-end cDNA library preparation (NEBNext Ultra II RNA Library Prep, Illumina). Multiplexed total cDNA libraries were sequenced on one lane using the NovaSeq 6000 instrument with 2x150 bp paired-end sequencing cycles.