Discovery of biological functions associated with Mtl1p mechanosensor of Saccharomyces sereviciae
Three mRNA samples each from wild-type and mtl1 strains (6 different biological samples in total) were analyzed by NGS. Each of the six samples was run individually.  For this experiment samples were barcoded using Ion XpressTM RNA-Seq Barcode 1-16 Kit from Life Technologies. Each sample was run in the Ion PGM Sequencer using the Ion 318 Chip V2 BC from Life Technologies.  The samples were prepared using the Ion PGMTM Hi-Q View Sequencing Kit