GSM6261353: Tamoxifen-injected Gli1creERT/Hhipflo/flox; Mus musculus; RNA-Seq
For isolating resident lung immune cells, 3 min before euthanizing each mouse, 3 μg of anti-mouse fluorophore-conjugated CD45 antibody diluted in 150 μl of 1X DPBS was intravenously injected via the retro-orbital injections. A digestion cocktail of Liberase TM (100 μg/mL, Sigma) and Dnase (50 U/ml, Sigma) in HBSS was used to dissociate the lung. CD45+CD45IV- resident immune cells are sorted by FACS. cDNA libraries were prepared for sequencing using standard protocol for the 10x Single Cell instrument, using Single Cell 3' v3 Chemistry