Illumina HiSeq 2500 paired end sequencing; Sequencing of RNAs derived from the purified endoplasmic reticulum of human LP1 myeloma cells expressing either the wt FAM46C protein or the D90G mutant allele
Sequencing of RNAs derived from the purified endoplasmic reticulum of human LP1 myeloma cells expressing either the wt FAM46C protein or the D90G mutant allele
Cell lines expressing inducible constructs of wt and mutant FAM46C alleles were induced by administration of 2microg/microl Doxycycline hydrocloride (Sigma cat no. D3447) for 5 days. Total RNA from ER fractions of FAM46C- and D90G-expressing LP1 cells was extracted using 1ml of TRIZOL reagent. Random primed cDNA libraries were constructed from 100 ng of total RNA.