GSM5495210: Lens, p7_WT_1; Mus musculus; RNA-Seq
Lenses were removed from P7 mice and immediately frozen on dry ice. Lenses were homogenized in a Qiagen RLT Plus buffer in a Bullet Blender and RNA was extracted using using Qiagen RNEasy Plus Micro kit. RNA was analyzed using Agilent 2100 Bioanalyzer and subjected to oligo dT selection before fragmentation and adapter ligation using Illumina TruSeq RNA Library Prep Kit PolyA plus RNA libraries were prepared for sequencing using standard Illumina protocols