GSM6044865: CAGEseq_HEK293_TNRC18_WT_2; Homo sapiens; RNA-Seq
For CAGE-seq experiment, qualities of total RNA were first assessed by Bioanalyzer (Agilent) to ensure that RNA integrity number (RIN) is over 7.0. The cDNA synthesis was conducted by using the total RNA and random primers. The ribose diols in the 5? cap structures of RNAs were oxidized and then biotinylated. The biotinylated RNA/cDNAs were selected by streptavidin beads (cap-trapping). After RNA digestion by RNaseONE/H and adaptor ligation to both ends of cDNA, double-stranded cDNA libraries (CAGE-seq libraries) were constructed.