Illumina paired end reads of Bacillus amyloliquefaciens strain B-1895 (library 1)
Bacillus amyloliquefaciens B-1895 total DNA samples were used for sequence determination by Illumina MiSeq platform. DNA libraries were prepared with the NEBNext DNA library prep kit for Illumina (New England Biolabs). Briefly, 1 microg DNA was sonicated, and fragments of 675 or 1,075 bp were selected. DNA ends were repaired, A-tailed, and ligated to adapters. Next, fragments were PCR amplified (8 cycles) and quality checked on a DNA 7500 chip on a 2100 Bioanalyzer (Agilent). Library sizes of 800 and 1,200 bp were isolated, validated (Bioanalyzer), and titrated with quantitative PCR (qPCR). After denaturation, the libraries were seeded on a flow cell (MiSeq v2, 2150 bp) at a density of 16 pM.