GSM5618184: Vitamin C, replicate 2; Laccaria bicolor; RNA-Seq
RNA was extracted using RNAzol® RT Reagent (MRC) according to recommendations of the vendor, treated with DnaseI (Thermo Scientific) as recommended by the vendor and >200 nt fraction was purified using RNA clean and concentrator-5 kit (Zymo research). Then, ribosomal RNAs were depleted using Ribo-Zero Gold rRNA Removal Kit (Yeast, Illumina) according to recommendations of the vendor, and >200 nt fraction was purified using RNA clean and concentrator-5 kit (Zymo research). Collibri Stranded RNA Library Prep Kit (Invitrogen™) for Illumina was used to prepare the libraries.