Rna-seq of Rhipicephalus microplus: rna pool from 15-20 ticks
The total RNA was extracted with TRIzol LS reagent (Invitrogen, Calrsbad CA) from 300l supernatant of each pool. The RNA-seq library was established according to the protocol of Illumina. Viral nucleic acid libraries were analyzed using an Illumina HiSeq3000 sequencer.