GSM5284817: LKO-LPS [SC2]; Mus musculus; RNA-Seq
Cells were then washed 2x with ice-cold PBS and then lysed directly with RLT buffer and stored in -80oC for RNA extraction on next day using QIAGEN RNA extraction kit with DNAse I treatment. Library preparation (TruSeq stranded RNA library kit) and sequencing was performed by Macrogen Inc. using an Illumina HiSeq. 2500 platform. TrueSeq Standard RNA library