GSM5531673: AB1_ChIP-seq; Nematostella vectensis; ChIP-Seq
embryos at gastrula stage were fixed with 2% formaldehyde for 12 min. Nuclei were collected by Dounce homogenizing the embryos. Chromatin was fragmented to an average size of 100-200bp using a Covaris S1 ultrasonicator library preparation according to Illumina ChIP-seq DNA Sample Prep Kit instructions (Catalog number IP-102-1001). The quantity and quality (size distribution) of the libraries was confirmed using an Agilent Bioanalyzer.