GSM4978590: 29DPI-Thal-Veh-NoTBI-2; Mus musculus; RNA-Seq
Animals were anesthetized and perfused with ice-cold filtered 0.9% saline solution, individual brain regions were removed (hypothalamus, thalamus, hippocampus), tissue triturated in Tri-reagent, RNA extracted utilizing Direct-zol RNA MiniPreps (Zymo Research, # R2025). Total RNA integrity was assessed using automated capillary electrophoresis on a Fragment Analyzer (Roche).  For all samples RQI > 8.0 and a total RNA amount of >75 ng was used as input for library preparation using the TruSeq Stranded mRNA Library Preparation Kit (Illumina, San Diego, CA, USA). Sequencing libraries were quantified by PCR using KAPA Library Quantification Kit for NGS (Kapa, Wilmington, MA, USA) and assessed for size distribution on a Fragment Analyzer.