GSM5332197: RNAseq14_AL_CPP; Homo sapiens; RNA-Seq
Samples in RLT were defrosted and gDNA plus RNA were isolated with AllPrep DNA/RNA Micro Kit (Qiagen, USA) following manufacturer's instructions. Samples in DMSO were defrosted, washed with PBS and suspended in lysis binding buffer (Ambion). Poly-A RNA was selected with Dynabeads Oligo (dT) (Ambion), and un-binded DNA was selected with SPRIselect beads, washed with EtOH and suspended in buffer EB. We used a 3' end RNAseq method optimized for generating libraries from low-input starting material (MARSeq), adapted for bulk RNAseq (Jaitin et al., 2014; Lavin et al., 2017) with minor modifications.