GSM4979411: Blastoid-nEND; NACL; Homo sapiens; RNA-Seq
Single cell suspension was washed with 1X PBS (0.04% BSA) before 10x Genomics procedure. The concentration of single cell suspension was adjusted to about 500 to 1000 cells/µL and was loaded on the 10x Genomics' Chromium™ system (10x Genomics, Pleasanton, CA) with the aim of 6000 to 10000 cells per channel (Chromium Next GEM Single Cell 3ʹ v3.1, catalog number 1000121). Single cell RNA sequencing libraries were constructed following the manufacturer's instructions (Zheng et al., 2017).