16S rRNA sequences of D. discoideum microbiome
The Qiagen DNeasy UltraClean Pro Kit was used to extract DNA. Samples were sent to Argonne National Laboratories (Lemont, IL, USA) for sequencing. Samples were sequenced on the Illumina MiSeq platform using 250bp paired-end cycles. Library preparation was performed using the 799F-1115R primer set to amplify the V5-V6 region of the 16S rRNA gene.