Phyllosphere of Pleurozium schreberi
Library preparation for Illumina sequencing was performed according to the manufacturers instructions for user-defined primers, with the exception of using the Qiagen HotStar Taq DNA polymerase and primers 515F-Y (5'-GTGYCAGCMGCCGCGGTAA-3') and 926R (5'-CCGYCAATTYMTTTRAGTTT-3') targeting V4-V5 regions of the nuclear 16S ribosomal RNA gene of bacteria for the amplicon PCR.