Transcriptome analysis of Marchantia polymorpha wild type and gun1 mutant spores
M. polymorpha spores were sterilised and plated on  Gamborgs medium supplemented with 1.2 % agar. The plates were placed under long day conditions for 40 hours, after which the spores were resuspended in 1 ml of sterile water, transferred into a microcentrifuge tube and spun down at 6,000 rpm for 1 minute. The water was removed, and the spore pellet flash-frozen in liquid nitrogen. Extraction of total RNA was carried out using the Direct-Zol RNA MINIprep kit (Zymo Research) following the manufacturers protocol. Transcriptome libraries of M. polymorpha wild-type and Mpgun1 mutant spores were prepared using 200 ng of DNase-treated total RNA as a template for the TruSeq Stranded Total RNA kit with Ribo-Zero Plant (Illumina).