GSM6261198: LN-hPSCs; Homo sapiens; RNA-Seq
HES-2-LN-hPSCs were dissociated into single-cell suspensions by treatment with Accutase. HES-2-primed hPSCs were first detached from the MEFs by treatment with Collagenase type IV, and further dissociated into single-cell suspensions by Accutase. Dissociated cells were filtered, centrifuged, and re-suspended in DMEM/F12 containing 10% FCS. To achieve cell viability of ˃ 90%, nonviable cells were removed using Dead Cell Removal kit. Viable cells were quantified after staining with Trypan Blue. Single-cell 3' gene expression libraries were generated from samples containing 8,000 cells (with predicted recovery of 5,000 cell), using Chromium Next GEM Single Cell 3ʹ GEM, Library & Gel Bead Kit v3.1 (10x Genomics), according to manufacturer's instructions.