GSM6258229: microglia in PLX-treated old mice (bio rep 3); Mus musculus; RNA-Seq
FACS sorted microglia cells were flash frozen in dry ice into 300 μL RNAprotect cell reagent (Qiagen).  Total RNA was extracted using RNeasy mini kit (Qiagen). Amplification of cDNA from the total RNA samples were performed using Ovation RNA-seq system kit (Tecan), following by low input RNA-seq library preparation.