WGS of SARS-CoV-2: Nasal Swab
The viral RNA was extracted from collection media and reverse transcribed to cDNA. Then the cDNA of the entire SARS-Cov-2 genome was amplified using Ion AmpliSeq SARS-CoV-2 panel. The amplicons generated were ligated to the Ion Barcode adaptors and sequenced using the Ion GeneStudio S5 sequencer. The raw sequencing data was analyzed using Torrent Suite software.