GSM5333249: 3 samples: KTx, HC, ESRD; Homo sapiens; RNA-Seq
peripheral blood was collected into EDTA-anticoagulant tubes, after red blood cells lysis, lymphocyte separation medium (TBD, LTS1077), blood and phosphate buffered saline 1X (Hyclone, SH30256.01) were slowly added to centrifuge tube separately at ratio of 2:1:1. After centrifuged at 450g for 20 minutes (min), PBMCs were aspirated from the middle of centrifuge tubes. Libraries were prepared according to Illumina's protocol