GSM6260648: Somatic cell, transitioning to primary, rep37; Mus musculus; RNA-Seq
Follicles were incubated in Accutase solution (Stemcell Technologies, Canada) (Zhang et al., 2018) until somatic cells partially detached from each other. Specifically, primordial and transitioning follicles were incubated in Accutase solution for 5 minutes, and primary and secondary follicles were incubated in Accutase solution for 10 minutes on 37 °C heated stage following agitation to dissociate somatic cells from the oocyte. Primordial and transitioning follicles were pipetted with 20 μm Pasteur pipet prepared by a micropipette puller (Sutter Instrument, CA, USA), primary follicle with a 30 μ