16S rRNA amplicon sequencing of hot phototrophic mat sample
Total DNA was extracted from triplicate ~250 mg samples using a DNeasy PowerSoil Kit (Qiagen, Carlsbad, CA, USA) according to the manufacturers instructions. Triplicate samples were pooled and submitted to the University of Minnesota Genomics Center (UMGC) for 16S rRNA amplicon sequencing targeting the V4 hypervariable region of bacterial and archaeal 16S SSU rRNA gene using the primers 515F and 806R following the methods in Gohl et al 2016