GSM5573188: SI LPL DKO (cDNA); Mus musculus; RNA-Seq
Single-cell RNA sequencing was performed on mesenteric lymph nodes (mLN), small intestinal intra-epithelial lymphocytes (SI-IEL), SI lamina propria lymphocytes (SI-LPL), colonic IELs and colonic LPLs isolated from Casp3/7Villin-Cre mice and Casp3/7FL/FL littermates. Single cells were isolated from the tissues by enzymatic digestion and live cells were sorted by fluorescence-activated cell sorting (FACS). The scRNA libraries were prepared using the GemCode NextGEM Single Cell 3' Gel Bead and Library kit, version 3.1 (10x Genomics, Cat. PN-1000121) according to the manufacturer's instructions. The cDNA content of pre-fragmentation and post-sample index PCR samples was analyzed using the 2100 BioAnalyzer (Agilent). Sequencing libraries were loaded on an Illumina NovaSeq flow cell at VIB Nucleomics core with sequencing settings according to the recommendations of 10x Genomics, pooled in a 90:10 ratio for the gene expression and hashtag antibody-derived libraries, respectively. Single-cell RNA sequencing