ddRADseq raw data of Mycale acerata sample Myc_HH127
DNA was extracted from ethanol preserved sponge tissue using the DNAeasy Kit (Qiagen) following manufacturer's recommendations. Library preparation was conducted following Peterson et al. (2012) with the modifications detailed by Combosch et al. (2017): Genomic DNA was digested using high-fidelity restriction enzymes EcoR1 and BfaI (New England Biolabs). Resulting fragments were ligated to custom-made P1 and P2 adapters containing sample-specific barcodes and primer annealing sites. Individually barcoded samples were then pooled into libraries and size-selected (270 to 600bp) using a Pippin prep (Sage Science). Each library was then PCR-amplified using Phusion Polymerase, and 14-18 PCR cycles. Resulting libraries were cleaned with to remove remaining adapters and primers using AMPure beads (0.8X volume ratio). Libraries were multiplexed and paired-end sequenced (150 bp) on an Illumina Novaseq 6000 at Novogene Europe (Cambridge, UK).