GSM5451959: Input ESRRB-piPSCs; Sus scrofa; ChIP-Seq
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ESRRB piPSCs were crosslinking with formaldehyde and chromatin was sonicated to 200-1000 bp, and then the sonicated chromatin was immunoprecipitated with the antibody FLAG. The DNA fragments were agarose electrophoresis quantified using Qubit 2.0 and sequenced on Illumina HiSeq2500 PE150 by SEQHEALTH Technology Corporation (China).