RNAseq of Oryzias latipes: osteoblast progenitors from caudal fins
RNA degradation and contamination was monitored on 1% agarose gels. RNA integrity and quantitation were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies). SMART-Seq v4 Ultra Low Input RNA amplification kit (Takara, 112219) was used to generate cDNA from low amount of RNA. Amplification was performed as per describe in manufacturer protocol. Library preparation is a proprietary information; hence we are unable to share information with you. After cDNA is fragmented and ligated with adapters, products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system.