GSM6304076: cADSC_BPA_Smp1; Canis lupus familiaris; RNA-Seq
We used the standard procedure of Qiagen RNeasy kit (Qiagen, Chatsworth, CA, USA) to extract total RNA from the cADSCs. We calculated total RNA concentration and assessed the integrity. The NovaSeq 6000 library for DNA sequencing was prepared using TruSeq Stranded mRNA Library Prep Kit (Illumina) following the protocol provided by the manufacturer. The libraries were quantified using KAPA Library Quantification kits for Illumina Sequencing platforms according to the qPCR Quantification Protocol Guide (KAPA BIOSYSTEMS, #KK4854) and qualified using the TapeStation D1000 ScreenTape (Agilent Technologies, # 5067-5582).