GSM5144799: 2017-059; Homo sapiens; RNA-Seq
The tissue samples were homogenized (6875D Freezer/Mill Spex). 50mg amount of sample was used for RNA isolation using the inhouse Tecan robot using AllPrep DNA/RNA/Protein kit (Qiagen) as mentioned in the protocol DOI:10.1007/978-1-4939-8695-8_19. 1 µg of total RNA was used for library preparation using TruSeq stranded mRNA library preparation kit (Illumina) as per the protocol provided by the manufacturer.