small RNAseq of CPM: Hatchling liver control 3
High quality total RNA samples were sent to the BC Cancer Agency, Vancouver (BC, Canada) for Small RNA RNA-Seq analysis. To ensure high-quality RNA was used for library construction of the miRNA library, an Agilent Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA) was used to measure RNA concentration, quality, and integrity. Small cDNA libraries were constructed, and quality was then assessed on the Agilent Bioanalyzer 2100 system. Sequencing was carried out using an Illumina HiSeq 2500 platform.