GSM5949870: U937_A2-2_6hr-10nM-H3B; Homo sapiens; RNA-Seq
1 million cells were collected in 350ul of Trizol and flash frozen. RNA was extracted with Zymogen Direct-Zol columns. 100ng of input RNA was used for library preparation with the KAPA RNA hyper prep kit with RiboErase treatment according to manufacturer's instructions. Libraries were sequenced on the Illumina NOVASeq in PE100 mode to a depth of 100M reads per sample.