GSM5285068: 4h Indolmycin treatment, 14hpi [B4]; Homo sapiens; RNA-Seq
Total RNA was collected from C. trachomatis infected cell cultures using Trizol. Total RNA was DNased then further purified to remove eukaryotic mRNA and both prokaryotic and eukaryotic rRNA using Oligotex, MicrobEnrich, MicrobExpress, and Ribozero. For S. pyogenes, total RNA was collected, DNased, and purified with Ribozero to remove rRNA. TruSeq® Stranded Total RNA Library Prep