Iso-Seq of chicken embryo
For each developmental stage, equal amounts of different lengths of cDNA (12, 23, and 36 kb) were pooled together to generate a full-length (06 kb) cDNA library. The resulting four full-length libraries from E1, E3, E5, and 10 were then sequenced on a PacBio Sequel platform using C3 reagents with four SMRT cells.