GSM5451742: Day 5.0 rabbit embryos; Oryctolagus cuniculus; RNA-Seq
The embryos were depellucidated using protease from Streptomyces griseus treatment and gentle mechanical dissociation. Cell singularization was achieved using trypsine 2 %, TryPLE 1 %, and gentle mechanical dissociation. Cell suspensions were loaded on a Chromium controller (10x Genomics, Pleasanton, CA, USA) to generate single-cell Gel Beads-in-Emulsion (GEMs). Single-cell RNA-Seq libraries were prepared using Chromium Single cell 3'RNA Gel Bead and Library Kit