GSM5448680: Postnatal_day1_hindgut; Mus musculus; ATAC-seq
Five to Thiry thousand cells were lysed and nuclei extracted in ATAC-Resuspension Buffer (RSB, 10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2) with 0.1% NP40, 0.1% Tween-20, and 0.01% Digitonin. DNA was transposed by Nextera Tn5 Transposase (Illumina) for 30min at 37C and purified with QIAquick column (Qiagen) NEBNext 2x MasterMix was used to pre-Amplify transposed DNA for 5 cycles and determine the required number of additional cycles by qPCR amplification. The final libraries were size selected (200bp to 800bp, including index) with AMpure beads Libraries were sequenced on a NextSeq500 instrument (Ilumina)