GSM4849758: SBI-756 total Rep 2; Homo sapiens; RNA-Seq
Cells were treated with 0.1mg/mL cycloheximide for minutes at 37℃, washed with PBS containing cycloheximide and lysed. Lysates were applied to a sucrose gradient and polysome analysis was performed. RNA was isolated from polysome fractions using standard RNA isolation protocol. RNA libraries were prepared for sequencing using standard Illumina protocols