HiC of Oikopleura dioica:tailbud
In situ Hi-C was performed according to (Rao et al. 2014), with modifications. Animals were fixed in 1% formaldehyde for 10 min at RT prior to DpnII digestion and bioitinylation and ligation. Libraries were prepared on streptavidin beads using NEBnext UltraII kit and size-selected by AMPure beads for 400 bp peak.