GSM5743378: JJDD005B; Homo sapiens; RNA-Seq
RNA samples were extracted from non-activated Jurkat cells or Jurkat cells activated for 5 hr with PMA + IONO, using the Direct-zol RNA Miniprep kit (Zymo Research). The libraries were prepared using TruSeq Stranded RNA LT Ribo-Zero Gold kit (Illumina) following the manufacturer's instructions, with two biological replicates. Cutadapt (version 2.6) with a minimum read length of 20, 5' end with a cutoff of 15 and the 3' end with a cutoff of 10 in paired-end mode was used to remove adapters. RNA-seq reads were pseudoaligned using kallisto v.0.46.0 run in quant mode with default parameters to estimate transcript abundance (transcripts per million, TPM). The transcript index for kallisto was made with default parameters and GENCODE Release 32 (GRCh38.p13) FASTA file.