GSM5494574: INPUT; Homo sapiens; RIP-Seq
RNA extraction was performed using QIAzol reagent and miRNEasy spin columns (QIAGEN), following manufacturer's instructions, and reverse transcription was performed with the Superscript VILO cDNA Synthesis Kit (Life Technologies) Ovation RNA v2 followed by Ovation Ultralow v2 kit was used to prepare cDNA libraries. Sequencing reaction were perfomred by Illumina NovaSeq 6000 Sequencing system.