RNA sequencing of Pachypeltis micranthus: whole body of adult male
The total RNA of male adult bugs was extracted with Trizol. The mRNA was enriched using magnetic beads with Oligo (dT). The obtained mRNA was broken into fragments by adding fragmentation buffer and was used for template, the first cDNA strand was synthesized by six-base random primers (random hexamers). Then, the second cDNA chain was synthesized by adding buffer, dNTPs, RNase H and DNA polymerase I. QiaQuick PCR kit was used to end repaired, A-tailed, and purified by adding EB buffer. Then the fragment size was selected by agarose gel electrophoresis and amplificated with PCR. The constructed library was sequenced using Illumina HiSeq platform.