RNA-Seq of bolA
After passing the test, ribosomal RNA was removed by RIBO-Zero kit to enrich mRNA.The mRNA broken into short fragments was used as template, reversed and purified into double-stranded cDNA, and then the second strand of cDNA containing U was degraded by USER enzyme.Purified double-stranded cDNA ends are repaired, A-tails are added and sequencing adapters are connected. Then AMPure XP beads are used to select segment sizes.Finally, PCR amplification was performed, and AMPure XP beads were used to purify the PCR products to get the final library