GSM5333204: Naïve B cell-HC1_Y20; Homo sapiens; RNA-Seq
RNA purification was performed using Qiagen RNeasy Kit (Valencia, CA). 50 pg of total RNA was used as input for the SMART-seq v3 cDNA synthesis kit (Takara). 1 ng of cDNA was used as input for the NexteraXT kit (Illumina) using 9 cycles of PCR amplification. Final libraries were quantitated by qPCR and sized distribution determined by Bioanalyzer prior to pooling and sequencing on a HiSeq2500 using 50bp PE chemistry. The RNA-seq libraries were made with Illumina HiSeq protocol.