GSM6216336: exo AALE, pBABE control, RNA, 1 [rnaEASY.aale.1.ctrl]; Homo sapiens; RNA-Seq
exoRNeasy serum/plasma maxi kit (Qiagen) was used to isolate extracellular vesicles, which were quantified using Nanoparticle Tracking Analysis (Malvern, UK). 30 ml of AALE cell culture supernatant was filtered to remove particles larger than 0.8 um. The filtrate was precipitated with kit buffer and filtered through a column to collect extracellular vesicles. These vesicles were then lysed with QIAzol® lysis reagent Smart Seq HT low input mRNA library prep kit (Takara). 2x150 PE reads.