GSM5379916: 1164Neonatal; Homo sapiens; RNA-Seq
Fresh intestinal tissue was cryopreserved per published protocol in freezing media (Fetal Bovine Serum + 10% Dimethyl sulfoxide). Samples were thawed and single cell suspensions made with dissociation on gentleMACS Octo Dissociator with heaters (Miltenyi Biotec). Live cells selected for using a live/dead kit. #' GEM libraries were used from 10X kit from Medgenome