GSM5572784: TWCM-11-74; Homo sapiens; RNA-Seq
Transmural tissue samples were removed from the left ventricle apex during LVAD implantation or after whole heart explantation. Samples for single cell were processed fresh by enzymatic digestion while samples for single nuclei were frozen in liquid nitrogen and stored at -80C until use and processed by mechanical homogenization. All single cell/nuclei suspensions were sorted by FACS to remove debris and dead cells. 10X Genomics Chromium Single Cell 5' Reagent V1.1.