GSM6260206: sgMLL3 CD44+CD104high, biological replicate 2 [sgMLL3#9-E/M]; Homo sapiens; ATAC-seq
100,000 cells were sorted based on the level of CD104. Nuclei are extracted from samples. Nuclei pellet is resuspended in the Tn5 transposase reaction mix. The transposition reaction is incubated at 37°C for 30 min. Equimolar Adapter1 and Adatper 2 are added after transposition, PCR is then performed to amplify the library. After the PCR reaction, libraries are purified with the AMPure beads.