RNA seq of human breast primary tumour: reads unmapped on human genome hg38
Before RNA extraction, tissue samples were ground to a homogenous powder in liquid nitrogen and immediately mixed with 700 l of DNA/RNA Shield. Total RNA was extracted using Quick DNA/RNA Miniprep Plus kit. For rRNA depletion, Ribogone-Mammalian kit was used. cDNA libraries were constructed using the SMARTer Stranded RNA-Seq Kit. Raw data were trimmed by trimmomatic, with following options: SLIDINGWINDOW:4:22 LEADING:22 TRAILING:22 HEADCROP:15 MINLEN:50. Subsequently, reads mapping to human genome hg38 were dropped from dataset.