GSM5771621: Pol II Standard ChIP; Homo sapiens; ChIP-Seq
For RNA-Seq, cells were directly lysed in Trizol, and extracted and DNase treated prior to quality control analysis as described in the text. For standard ChIP-Seq, cells were fixed in 1% formaldehyde, harvested, and frozen as described in the text. Chromatin was sheared and ChIP was performed as detailed in the methods. For DFF-ChIP, native isolated nuclei or crosslinked cells were harvested as described in the methods, and chromatin was digested with DFF for 1 hour at 37° C. Soluble chromatin was extracted and ChIP was carried out as described in the methods. Details of library construction for each type of assay are provided in the methods.