GSM5692721: spleen M-MDSCs WT replicate 3; Mus musculus; RNA-Seq
M-MDSCs and G-MDSCs were sorted from spleens of B16.F10 melanoma bearing Wild Type and Nrlp3-/- mice, and total RNA was extracted using Macherey-Nagel NucleoSpin RNA kit as described by manufacturer's protocol (NucleoSpin® RNA). Each RNA sample was representative of one mouse. NGS libraries were generated using 300 ngr total RNA as input on average with the QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina kit from Lexogen according to manufactures protocol, using 15 or 17 cycles of amplification. Libraries were sequenced on Illumina Nextseq 500 on 1 x 75 High flowcell. QuantSeq 3′ mRNA-Seq Library