GSM5906381: healthy bee 1; Apis mellifera; RNA-Seq
RNA samples that were extracted using TRIzol reagent (Invitrogen, Carlsbad, CA) were further processed with a Qiagen RNeasy purification kit (Germantown, MD) according to the manufacturer's instructions. The quantity and purity of RNA samples were measured using a NanoDrop 8000 Spectrophotometer (NanoDrop Technologies, Wilmington, DE). The integrity of RNA samples was evaluated using Agilent Bioanalyzer 2100 (Santa Clara, CA). Only RNA samples with an RNA integrity number (RIN) value of over eight were used to generate first and second-strand cDNAs using random hexamer primers and the reverse transcriptase Superscript II (Invitrogen, Carlsbad, CA) following manufacturer's instructions. The overall level of ribosomal RNA present in total RNA samples was reduced prior to library construction using the Ribo-Zero™ Magnetic (Human/Mouse/Rat) rRNA Removal Kit (Epicentre Technologies, Madison, WI). The TruSeq RNA Sample Prep kit (Illumina, San Diego, CA) per manufacturer's protocol.