16SrRNAgenesequencingoftheV4-V5regionfroma no template control run outside of the sample plate during PCR amplification
V4-V5regionofthe16SrRNAgenewasamplifiedfromaquariumbiofiltersamplesusingIlluminaprimerswithuniquebarcodesontheforwardandreverseprimerstoallowformultiplexingofthesamples