GSM5994342: N4; Homo sapiens; miRNA-Seq
The serum was collected from the whole fresh blood and frozen to -80℃.The serum microvesicles of five acute ischemic stroke (AIS) and healthy controls was purified using RiboTM Exosome Isolation Reagent (for plasma or serum) and analyzed by flow cytometry and nanoparticle tracking analysis (NTA).The RNA from serum microvesicles were extracted using MagZolTM Reagent for quality inspection using Keo K5500/Qubit 2.0 and Agilent 2200 TapeStation. 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using NEBNext® Multiplex Small RNA Library Prep Set for Illumina®