PolyA RNA-Seq in HSV-1 infected human fibroblasts
MRC5 cells were mock-infected or infected with HSV-1 (KOS) at an MOI of 10 PFU/cell for two, four, six, eight, or twelve hours. PolyA-selected RNA-Seq libraries were generated. Illumina sequencing was performed using HiSeq2500 to generate 50 SE reads, adaptors are Illumina TruSeq 6 base.