GSM4982995: B7; Mycobacteroides abscessus; RNA-Seq
RNA isolation was performed using the Nucleospin RNA kit (Machery Nagel, Düren, Germany), with slight modifications. Following isolation, RNA integrity was determined, ribosomal RNA was depleted and the mRNA library was constructed and sequenced on a NextSeq 500 (Illumina, San Diego, CA). RNA libraries were prepared for sequencing using standard Illumina protocols