GSM5692481: YAP_2; Homo sapiens; ChIP-Seq
DNA was purified using phenol-chloroform-isoamyl alcohol extraction and ethanol precipitation as well as RNase A treatment. For library amplification, 24 μl of DNA was mixed with 1 μl of TruePrep Amplify Enzyme , 10 μl of 5× TruePrep Amplify Enzyme buffer and 5 μl of ddH2O, as well as 5 μl of uniquely barcoded i5 and i7 primers from TruePrep Index Kit V2 for Illumina (Vazyme). The DNA fragments were then isolated and sequenced using the Illumina HiSeq X-ten platform (Shanghai Biotechnology Co., China).