GSM4982239: Normal_TZ; Mus musculus; RNA-Seq
After tissue dissection and dissociation, FACS purified suspended cells were partitioned into nanoliter-scale Gel Bead-In-Emulsions (GEMs) with the Chromium Single Cell Controller (10XGenomics) Cell encapsulation, barcoding, and library preparation followed the standard scRNAseq protocol comprising reverse transcription, amplification, and indexing (10xGenomics). Sequencing was performed using a NextSeq Illumina device (Illumina).