GSM5385875: Negative_control; Mus musculus; ChIP-Seq
Briefly, cells were cultured in 20 cm dishes and stimulated using IL-6. Chromatin (5 μg) was prepared using 4 rounds of sonication (30 sec on and 30 sec off) in precooled conditions. DNA disrupted into fragments of 200-1000 bp was immunoprecipitated using Protein-G Dynabeads (Novex) bound with an anti-HA antibody to detect Arid5a (4 μg each). Normal IgG (4 μg) was used as a control, and libraries were constructed from immunoprecipitated DNA. The ChIP-Seq library of precipitated DNAs was prepared using KAPA Library Preparation Kits for Illumina (Kapa Biosystems).