Illumina HiSeq 2000 sequencing; Effect of treatment OPM2 multiple myeloma cells with EPA, DHA or bortezomib
Effect of treatment OPM2 multiple myeloma cells with EPA, DHA or bortezomib
Cell lines from culture Data were analyzed using Novosmart software (Novogene, Cambridge, UK). Kyoto Encyclopedia of Genes and Genomes (KEGG) (Novosmart; Cambridge, UK) and Reactome (www.reactome.org) were used to perform enrichment analysis of the DEGs. OPM2 cells were seeded in a 12-well plate at density of 10 × 103 cells/well and incubated with the indicated concentrations of compounds. Cells were treated with 50 µM of DHA/EPA (6 h) or 10 nM of bortezomib (4 h) Data were analyzed using Novosmart software (Novogene, Cambridge, UK). Kyoto Encyclopedia of Genes and Genomes (KEGG) (Novosmart; Cambridge, UK) and Reactome (www.reactome.org) were used to perform enrichment analysis of the DEGs. Total RNA was extracted from cells using RNeasy Mini Kit (74104, Qiagen, Hilden, Germany) and RNA integrity was determined by Agilent 2100 analysis.  Novogene