GSM4981863: RNA-seq_p11_4; Rattus norvegicus; RNA-Seq
Total RNA from 700,000 to 1.25 million RGCs per sample was isolated using the Qiagen RNAeasy kit from Qiagen, which included a 15 minutes On-column DNAse step. Ribodepletion and RiboErase (human, mouse, rat) were used to remove ribosomal RNA. Libraries were prepared according to library kit manufacturer's protocol, indexed, pooled, and sequenced on an Illumina HiSeq. All samples were sequenced in the same lane.