GSM5383083: RHP4515 scRNA-Seq; Homo sapiens; RNA-Seq
Cells isolated from organs were indexed-sorted using the Indexed-sorting panel on a BD FACSARIAIII (BD Biosciences) into 96 well plates containing 3 µL Lysis buffer using a 70 µm nozzle Single cell cDNA libraries were made using the SMARTSeq v2 protocol (Picelli et al., 2014) with the following modification: Use of 200 pg cDNA with 1/5 reaction of Illumina Nextera XT kit (Illumina, San Diego, CA, USA). All samples were subjected to an indexed paired-end sequencing run of 2x151 cycles on an Illumina HiSeq 4000 system (Illumina, San Diego, CA, USA) (300 samples/lane).