GSM5835309: jmj26 + Alt JMJ26 Rep1; Arabidopsis thaliana; Bisulfite-Seq
Genomic DNA was extracted using GeneJET Plant Genomic DNA Purification Kit (Thermo Scientific) and sheared by E220 Focused-ultrasonicator (Covaris) to target average fragments around 350 bp. Sequencing libraries were prepared using NebNext Ultra II DNA Library Prep Kit (New England BioLabs) with Methylated Adaptor (New England BioLabs). Adapter-ligated DNA was carried through the bisulfite conversion using EZ-96 DNA Methylation-Gold™ MagPrep Kit (Zymo Research). Bisulfite-treated samples were amplified by EpiMark Hot Start Taq DNA Polymerase and indexed with NEBNext Multiplex Oligos for Illumina (New England BioLabs).