GSM5335524: FISC_RNA-seq; Mus musculus; RNA-Seq
RNA from quiescent,freshly isolated or culture muscle stem cells was harvested using Trizol reagent. RNA libraries were generated and sequenced with DNBseq platform by BGI, Hong Kong. In brief, rRNAs were first depleted from total RNAs and the remaining RNAs were fragmented and then reverse transcribed. The resulting DNA fragments were end-repaired, A-nucleotide added and adaptors ligated. The resulting libraries were heated to seperate into ssDNA and further cyclized to make DNA nanoballs (DNBs). DNBs were sequenced by DNBSEQ-G400 sequencer in BGI-Hong Kong.