GSM4449120: MUC5307_6881_IP_HEP_Frozen_IP_fasted; Mus musculus; RNA-Seq
Whole liver nuclei as well as bead-bound and supernatant nuclei prepared from INTACT mice were directly resuspended in Trizol. RNA purification was performed using EconoSpin columns (Epoch) with on-column DNase digestion (Qiagen). Total RNA (50-100ng) was prepared for sequencing using VAHTS Stranded mRNA-seq Library Kit for Illumina (Vazyme NR602) following manufacturer's recommendations, except that no selection for poly-adenylated RNA was used. Prepared PCR products were purified and size-selected on AMPure XP beads (Beckman A63881), and library quality was assessed on the Agilent Bioanalyzer 2100 system. RNA libraries were sequenced as 50 bp paired-end runs on a HiSeq 4000 platform (Illumina, ‎San Diego, USA).