Full-length transcriptome obtained with the Iso-Seq method from a pool of 13 paralarval octopus vulgaris brains (one day post hatching)
Large scale PCR was done to amplify the cDNA with the PrimeSTAR GXL DNA Polymerase as described in the protocol: Iso-Seq Template Preparation for Sequel Systems. Poly(A)-tailed mRNA was selected and no size-selection was performed. After the large scale PCR the pool was enriched for larger fragments by using beads as described in the protocol.  The NEBNext cDNA Synthesis & amplification kit  (https://www.pacb.com/wp-content/uploads/Procedure-Checklist-Iso-Seq-Express-Template-Preparation-for-Sequel-and-Sequel-II-Systems.pdf) was used