GSM5024787: HC10; Homo sapiens; RNA-Seq
Peripheral whole blood was drawn via venepuncture. Neutrophils were isolated using Polymorphprep (Axis-Shield PoC AS, Norway) by density centrifugation according to the manufacturer's instructions.Total RNA was extracted from freshly isolated neutrophils using TRIzol reagent (Invitrogen, CA, USA). The sequencing library was established using an NEBNext® Ultra™ RNA Library Prep kit for Illumina® (NEB, USA) and then sequenced on the Illumina HiSeq 2000 platform according to the manufacturer's instructions. RNA libraries were prepared for sequencing using standard Illumina protocols