GSM5145697: Embryoids_d8; Mus musculus; RNA-Seq
90 d8 embryoids were pooled and dissociated in accutase (Gibco: #A11105-01) for 30min at 37c with pipetting every 5 minute intervals. Cells were filtered using 40um flowmi cell strainers (Belart cat 136800040).Cells were then fixed in prechilled methanol for 30 min on ice  and stored at -20c. Cells were rehydrated in 1xDPBS -1.0%BSA -0.5U/ul RNASE inhibitors and filtered again to eliminate debris and cell agglomerates. Cell were immediately counted using the Countess 3 automated cell counter (Thermofisher scientific) and processed for 10X Genomics scRNA-seq Single-cell libraries were generated following the manual instructions of 10x Genomic (Chromium‚single cell 3'V3) RNA-seq