GSM5288473: BN_MB6_F23_RNA; Mus musculus; RNA-Seq
whole brain and liver were removed, flash frozen on dry ice, and 100mg of each tissue was used for a TRIzol RNA extraction. Briefly, 1 mL of TRizol was added to each sample, chloroform was used for phase separation of the aqueous phase containing RNA, RNA was precipitated using isopropanol and washed with 75% ethanol. Purified RNA was enriched for PolyA using oligo-dT beads, and Stranded mRNA-seq libraries were prepared.