Peripheral blood whole exon sequencing
The genomic DNA from peripheral blood was used as the detection material. First, the DNA was broken and the library was prepared. Then, the TARGET gene exons and DNA in the adjacent shear zone were captured and enriched using THE BGI V4 chip, and finally, the mutation was detected using the MGISEQ-2000 sequencing platform.The quality control index of sequencing data was as follows: the average effective sequencing depth of the target area was 100X, among which the average depth of the target area was >20X locus accounting for >95%.