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Dissected samples were subjected to RNA extraction using the RNeasy Micro Kit. The SMART-Seq v4 Ultra low input RNA Kit was used for first-strand and second strand cDNA synthesis and double-stranded cDNA end repair. cDNAs were purified using the AMPure XP. The libraries were generated by PCR enrichment of the adaptor-ligated DNA. The concentration and quality of libraries were measured by the Agilent High Sensitivity DNA Kit and a Bioanalyzer 2100 from Agilent Technologies. The libraries were sequenced using Hiseq4000.Three biological replicates were performed for each genotype or treatment.