GSM5573806: SOCS2-dil10_S2; Mus musculus; RNA-Seq
The fourth pair of lactating mammary glands were harvested from the mice and the inguinal lymph nodes carefully removed from the tissue preparation. Each mammary gland was finely sliced and digested with Liberase at and DNAse I. Lactating mammary gland cell suspensions of each mouse were labeled using TotalSeq™ anti-mouse hashtag 1-3 antibodies A301-303 (Biolegend, UK) and were pooled and stained with anti-IgG2b-Vioblue antibody to specifically label CD45 cells. Doublet, 7AADpos and FITCpos cells were gated out to sort only viable resident CD45pos immune cells using a BD FACS Influx instrument (BD Biosciences).