GSM5386010: GSC90-Ctrl replicate 3; Homo sapiens; RNA-Seq
Total RNA was extracted from cells using TRIsure (Bioline) and treated with DNase I RNase-free (Thermo Scientific). 700ng of total RNA were used to prepare RNA sequencing libraries, using the Illumina TruSeq Stranded mRNA Library preparation kit. Sequencing was carried out using 2x100 cycles (paired-end reads of 100 nucleotides each) on an Illumina NovaSeq 6000 system (S1 flow cells).