GSM5501544: PD0_scRNAseq_WT_Rep3; Mus musculus; RNA-Seq
Freshly dissociated unsorted skin cells from mouse skin were subjected to scRNA-seq library preparation. For scRNA-seq, dermal and epidermal single-cell suspension were counted with hemocytometer and mixed with 1:1 ratio. Dead cells were removed using the MS columns of the Dead Cell Removal Kit (MACS) as per manufacturer's directions. Library construction were processed according to Chromium Single Cell 3' Reagent Kits v3 Chemistry (10X Genomics) as per the manufacturer's protocol. Library was sequenced using Illumina HiSeq X Ten platform (Illumina).