Bacterial host range of tetO in WWTP influent samples sequenced by epicPCR + amplicon sequencing via MinION, ONT
Amplicons were checked on size using electrophoresis and measured the concentration using Qubit. Amplicons from different samples were normalized, barcoded and sequenced according to the ONT protocol "Native barcoding amplicons (with EXP-NBD104, EXP-NBD114, and SQK-LSK 109)". Obtained reads were filtered through nanofilt (https://github.com/wdecoster/nanofilt; score > 7) for the initial QC.