GSM5384675: Radial glia cells - full_length_Notch_Rep3; Mus musculus; OTHER
After dissection of electroporated cortex, gDNA was extracted using the QiAmp DNA Micro kit. The gDNA was digested with DpnI, adaptors were ligated and then digested with DpnII and amplified by PCR (Marshall et al, Nat Protoc, 2016). After gDNA extraction and DamID, DNA was sonicated to an average size of 300bp using a Diagenode Bioruptor and DamID adaptors removed with AlwI digestion. Sonicated material was end-repaired, A-tailed, sequencing adaptors ligated and PCR amplified (see Marshall et al., Nat Protoc, 2016).