GSM4981444: 368: H3K36me3 ChIP-seq, Primary FLS, Unstimulated; Homo sapiens; ChIP-Seq
Chromatin was prepared on pellets of fixed cells  using the iDeal ChIP seq kit for Histones (Diagenode). ChIP assays were performed using 1 million cells per IP and the following antibodies: H3K4me3 (0.5 μg, C15411003, Diagenode), H3K27me3 (1 μg, C15410195, Diagenode) and H3K27ac (1 μg, C15410196, Diagenode). A control library was processed in parallel using the same amount of control Diagenode ChIP'd DNA. Libraries were prepared from 1 ng of IP and input DNA using the MicroPLEX v2 protocol, quantified by BioAnalyzer, purified (AMPure beads) and eluted in TE.