GSM5453368: leukocytes-normal diet; Mus musculus; RNA-Seq
The tumor tissues were collected 14 days post-implantation and single cell suspensiones were prepared. Dead cells were removed from the single cell suspension using the Dead Cell Removal Kit (Miltenyi Biotec, Cat# 130090101). CD45+leukocytes were magnetically labeled and enriched using magnetic beads (Miltenyi Biotec). The isolation procedure was performed according to the manufacturer's instructions. Library preparation was conducted according to the manufacturer's instructions using Chromium Next GEM Single Cell 3' Gene Expression Kit v3.1.  Single indexed, paired-end libraries were sequenced on an Illumina HiSeq2500.