WGS of freshwater sediment: iron ore-disturbed sediment
Total DNA was extracted from the composite samples (1 g) using the E.Z.N.A. Soil DNA kit (OMEGA bio-tek, Norcross, GA) following the manufacturers instructions. DNA quantification was determined using a Qubit fluorometer (Thermo Fisher Scientific, Carlsbad, CA). Shotgun libraries were built with Nextera DNA Flex Library Prep kit, according to manufacturers instruction, and sequenced in two separated runs at NextSeq platform with NextSeq 500/550 High Output Kit v2.5 (150 cycles) (Illumina, San Diego, CA).