GSM5333702: 135-011_placeboC_Baseline; Homo sapiens; RNA-Seq
Total RNA was isolated from fixed tissue specimens using High Pure RNA Paraffin Kit (Roche) and quantified by qubit (Thermo Fisher). Only RNA samples with DV200 > 30% by Agilent 2100 bioanalyzer were used. RNA-Seq libraries were prepared by using TruSeq RNA Access kit following manufacturer's protocol. Targeting genome-wide coding regions by capture probes. Single-end read of 75bp was performed by NextSeq genome sequencer (Illumina).