GSM5834227: C_37; Mus musculus; RNA-Seq
Mice were scarified and heartblood was collected. 400 µl blood was transferred to RNAprotect Animal Blood Tube and RNA was isolated using RNeasy Protect Animal Blood Kit from Qiagen according to manufacturer instructions. Globin mRNA was depleted from total RNA using GLOBINclear kit by Thermofisher, a strand-specific RNA sequencing library was generated using NEBNext® Ultra  II Directional RNA Library Prep Kit (New England Biolabs) according to manufacturer's protocols. Quality and integrity of total RNA was controlled on Agilent Technologies 2100 Bioanalyzer (Agilent Technologies; Waldbronn, Germany). PolyA+RNA were purified  from 1µg total RNA using Dynabeads mRNA DIRECT Micro Kit (ThermoFisher). The RNA Sequencing library was prepared with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs).  The library was sequenced on Illumina HiSeq 4000 using HiSeq 3000/4000 SBS Kit (150 nucelotides, paired end) with an average of 60M reads per RNA sample.