Methyl-Seq of mus musculus: primary microglia
Bisulphite conversion of genomic DNA was conducted and Bisulphite-converted DNA was purified and used to prepare a sequencing library. In this procedure, to synthesize DNA containing a specific sequence tag, DNA is randomly primed using a polymerase able to read uracil nucleotides. The libraries are diluted and loaded onto DNA Cluster Generation System. After cluster generation, the flow cell is transferred to the Hiseq System for sequencing using 100 bp paired-end reads.