GSM5745491: LJ_9_WT_211_1m_M; Mus musculus; RNA-Seq
Total RNA was isolated from thymuses of 1 month-old mice (3 males and 3 females of WT and Axl -/-Mertk-/- mice) using the NucleoSpin RNA kit (Macherey-Nagel). The quality of the isolated total RNA was assessed using Agilent TapeStation 4200 RNA-Seq libraries were prepared with 500 ng total RNA using the TruSeq stranded mRNA Sample Preparation Kit according to the manufacturer's protocol (Illumina). RNA-seq libraries were multiplexed, normalized and pooled for sequencing. The libraries were sequenced on the HiSeq 2500 system (Illumina) at single read 50bp.