GSM6199168: MeDIP_FA4-IP; Mus musculus; MeDIP-Seq
Genomic DNA was extracted and fragmented to 100-300 bp using DNeasy kit (QIAGEN Inc., Germany). Adapter sequences (Illumina, Inc., USA) were added to the fragmented DNA. Heat-denatured to single-stranded DNA sequences were immunoprecipitated with 5mC antibody (Diagenode, USA), and the pellet was purified. Library amplification and purification reactions were performed on IP DNA that had been immunoprecipitated and input DNA that had not been immunoprecipitated.