Miseq for base editing mouse
amplicons for Apc.1405, Smad4.224.[Small intestine 2D cells lenti gRNA 1405 12hr dox replicate 2] [si HOM organoids targeted with 1405/135/224/545 synthetic gRNA EN (remove RspoI) + nutlin + Tgfbeta replicate 2 224 PCR]