NextSeq 550 paired end sequencing; Whole-genome sequencing of SARS-CoV-2 samples in Sweden during early 2022
Whole-genome sequencing of SARS-CoV-2 samples in Sweden during early 2022
Nasopharyngeal swab samples were collected in 0.9% saline solution and subjected to heat inactivation by 70 Â°C for 50 min in a hot air oven. RNA extraction prior to SASR-CoV-2 WGS library preparation was performed using the KingFisher Flex System and the MagMAX Viral/Pathogen II Nucleic Acid Isolation Kit. Libraries were constructed using Illumina COVIDSeq Test kits according to the manufacturers instructions.