RNA-Seq of T24
Total RNA was extracted from cell/tissues using the Trizol (invitrogen) according to the manufacturers protocol, and ribosomal RNA was removed using the Ribo-Zero kit (Epicentre, Madison, WI, USA).FragmentedRNA (the average length was approximately 200 bp) were subjected to first strand and second strand cDNAsynthesis following by adaptor ligation and enrichment with a low-cycle according to instructions of NEBNextUltra RNA Library Prep Kit for Illumina (NEB, USA). The purified library products were evaluated using theAgilent 2200 TapeStation and Qubit2.0(Life Technologies, USA).