GSM5288516: TAMs 198 (shChi3l1 rep 3); Mus musculus; RNA-Seq
Tumors were separated and homogenized for 15 min at 37°C in Collagenase IV Cocktail and red blood cells were lysed using ACK lysing buffer. Cells were Cells were incubated with 1.0 µg of TruStain fcX (BioLegend) for 10 min on ice to block Fc receptors, followed by staining with the combination of indicated antibodies and subject to FACS to sort macrophages. First, cDNA was generated from 10ng RNA using Smart-seq ultra Low input RNA kit from Takara. Second, libraries were generated from 1ng of cDNA by using Nextera XT from Illumina