GSM5213752: Horse 4, timepoint 48 h; Equus caballus; RNA-Seq
Biopsies at 0 h were taken in duplicate (per animal) from the uterine horn bifurcation at the abattoir, placed into 1.5 mL of RNALater (10437114, Fisher, UK) in RNAse/DNAse free tubes, kept at 4 °C for 24 h. RNALater was removed from the tubes and tissues were stored at -80 °C until extraction. The explant culture was performed in triplicate for timepoint per animal. After periods of 24, 48 and 72 h explants were removed from the well and stored in 1.5 mL of RNALater for a 24 h period at 4 °C, RNALater removed and explants stored at -80 °C for future RNA extraction. Total RNA was extracted from endometrial samples using the GeneJET RNA Purification kit (Thermo Scientific, UK) following the manufacturer's instructions. cDNA synthesis, fragmentation and library preparation were performed using the Illumina TruSeq Stranded mRNA kit #20020594 according to the manufacturer's instructoins to prepare the dual-indexed next-generation sequencing libraries