GSM5145540: SLE3_KIR-; Homo sapiens; RNA-Seq
Live KIR+ or KIR- CD8+ T cells were bulk sorted directly into 350 μL TRIzol (Qiagen) by FACSAria Fusion flow cytometer (BD).  Total RNA was extracted from TRIzol samples using chloroform separation and isopropanol precipitation and then RNAeasy Plus Mini kit (Qiagen) for clean-up. After analysis on the 2100 Bioanalyzer, the sequencing libraries were prepared using the RiboGone Mammalian rRNA Depletion Kit (Clontech) and the SMARTer Stranded RNA-seq Kit (Clontech). The resulting library was sequenced on the HiSeq 4000 platform (Illumina) in Stanford Functional Genomics Facility. For each sample in the bulk RNA sequencing library, 75-base-pair paired-end reads were acquired from the sequencer.