RNA-seq of Arabidopsis: Col-0 wild-type shoot rep 3
RNA sequencing was performed by the Novogene company in China. The RNA was extracted with QIAGEN plant RNA kit. The RNA-sequencing transcriptome library was prepared using the  NEBNext Ultra RNA Library Prep Kit for Illumina. After adenylation of 3 ends of DNA fragments, NEB Next Adaptor with hairpin loop structure were ligated to prepare for hybridization. In order to select cDNA fragments of preferentially 150~200 bp in length, the library fragments were purified with AMPure XP system. Library quality was assessed on the Agilent Bioanalyzer 2100 system.