GSM6081171: Mesothelioma tumor, ADU-S100 10µM; Homo sapiens; RNA-Seq
After 24 hours of treatment in a ULA dish, tumor spheroids were digested with trypsin in a 37°C incubator for 5 minutes to obtain single-cell suspensions. Cells were loaded onto a 10x chromium instrument (10x Genomics) per manufacturer's instructions ScRNA libraries were generated using the single cell 3′ reagent kit (10x Genomics) per the user guide