GSM5333425: MECv 10X scRNA-seq; Mus musculus; RNA-Seq
Mammary gland were digested into single cells, the Dyeflour450-BV421-, CD45-APC-, CD31-APC-, Ter119-APC-, CD29-FITC+, CD24-PeCy7+ cells were sorted by FACS. for Nfatc1+ cells, the RFP+ cells were sorted by FACS. Single cells were captured using the Chromium Platform (10X Genomics) and libraries were generated using Single Cell 3' v2 chemistry. Cell capture, library preparation, quality control, and sequencing was performed at beijing capitalBio technology