GSM5692812: AP2-Sp ChIP-seq experiment-2 INPUT; Plasmodium berghei; ChIP-Seq
Mosquito midgut was dissected and fixed immediately in 1% paraformaldehyde solution and kept in it until dissection of all mosquitoes were completed.They were sonicated with Bioruptor in the following condition: total of 20 times at 30 s in middle power in the cell lysis solution containing 1% sodium dodecyl sulfate. The supernatant was subjected to immunoprecipitation with anti-GFP antibodies using Dynabeads protein A. Libraries were constructed using library preparation kit for SOLiD 5500 system in experiment 1 and KAPA Hyperprep kit (KAPA Biosystems) in experiment 2, respectively