GSM5213771: wild type Top2-myc9 t0 WCE; Saccharomyces cerevisiae; [Candida] glabrata; ChIP-Seq
Cells were opened by FastPrep-24™ 5G Instrument (MP Biomedicals) followed by sonication with Bioruptor® Pico instrument. After removing the cell debris, the supernatant was used for the chromatin immuno-precipitation with anti-myc 9E11 antibody. To reverse crosslink, all the samples were incubated in 65°C overnight. DNA purification was conducted by regular phenol/chloroform/isoamyl alcohol extraction. Library preparation was performed using NEBNext® Ultra II DNA Library Prep Kit according to the manufacturer´s protocol. Amplified library DNA was purified and subjected to ~140 bp - 800 bp size selection using Agencourt AMPure XP beads (Beckman Coulter) and quantified by NanoDrop™ 3300 Fluorospectrometer and Agilent 2100 Bioanalyzer.