GSM5452713: P14 cells from PBMC; Mus musculus; RNA-Seq
Briefly, FACS-sorted CD8+ T cells were suspended in DPBS containing 0.04% BSA and captured in droplets using a Chromium Single Cell Controller instrument (10x Genomics). After reverse transcription and cell barcoding in droplets, cDNA was purified and PCR amplified, followed by fragmentation, end-repair, double-size selection and PCR amplification with sample-indexing primers. The single-cell RNA-seq (scRNA-seq) libraries were prepared using Chromium Next GEM Single Cell 3' reagent Kits (10x Genomics) according to the manufacturer's instructions.