Drosophila w[1118] 3rd instar larval hemocytes
3rd instar Hemocytes were isolated by manual dissection in batches of 50 larvae and stored at -80C. Samples from 5000 larvae were pooled and total RNA extracted by Trizol extraction. RNA was further purified and DNase1 treated prior to clean-up using RNeasy Mini columns (Qiagen). CAGE libraries were prepared by DNAForm (Riken, Japan).3rd instart Hemocytes were isolated by manual dissection in batches of 50 larvae and stored at -80C. Samples from 5000 larvae were pooled and total RNA extracted by Trizol extraction. RNA was further purified and DNase1 treated prior to clean-up using RNeasy Mini columns (Qiagen). CAGE libraries were prepared by DNAForm (Riken, Japan).