GSM6217382: Muscle.7d.IgG.Muscle.#1; Mus musculus; RNA-Seq
For flow-cytometrically sorted samples, cells were isolated by first enzymatically digesting the indicated tissue and then purified by fluorescence-activated cell sorting (two times to achieve enhanced purity). A total of 1,000 cells were sorted into TCL buffer with 1% 2-mercaptoethanol and submitted for library construction and sequencing. For whole muscle samples, tissue was homogenized in Trizol, and then RNA was isolated per manufacturer protocol. For each of these samples, 2 ng of purified RNA was added to TCL buffer with 1% 2-mercaptoethanol and submitted for library construction and sequencing.