GSM5066758: ESC_rep1; Homo sapiens; RNA-Seq
Total RNA was extracted from 1 x 10^6 cells using the RNeasy Plus Mini. Kit (Qiagen) as per manufacturer's instructions. RNA quality (RIN >8) was checked via Bioanalyzer (Aglient) and RNA concentration determined using a Qubit fluorometer (Thermofisher). Libraries were prepared from 1µg RNA using the NEBNEXT Ultra8482 II Directional RNA Library Prep Kit for Illumina (E7760S) in combination with the NEBNExt Poly(A)mRNA Magnetic isolation module (E7490S) as per manufacturer's instructions.