TMC7 metagenome
Total DNA was extracted using the E.Z.N.A. Soil DNA kit (Omega, USA) according to manufacturers instructions. DNA quantity and purity were detected using NanoDrop2000 microspectrophotometry (Thermo Fisher, USA), and DNA integrity was assessed with 1% agarose gels. About 200 ng of total DNA was used to prepare 300 bp DNA library using TruSeqTM DNA Sample Prep Kit (Illumina, USA). DNA sequencing was performed on Illumina HiSeq 2500 platform (Illumina, USA) by Majorbio (Majorbio, China).