GSM5288507: primary LSECs infected with siAirn1_2, biological rep2; Mus musculus; RNA-Seq
Primary LSECs were isolated by pronase/collagenase perfusion digestion from the 8-week-old Balb/c mice liver, subsequently Primary LSECs (1x106/well) were seeded in collagen–coated 6-well plates and transfected with siAirns and siRNA-control for 48 h, RNA of cells were harvested and isolated useing Trizol reagent. Total RNA was qualified and quantified using a Nano Drop and Agilent 2100 bioanalyzer The library was amplified with phi29 to make DNA nanoball (DNB) which had more than 300 copies of one molecular, DNBs were loaded into the patterned nanoarray and single end 50 bases reads were generated on BGIseq500 platform (BGI-Shenzhen, China).