GSM5501706: gfi1b mutant rep1; Danio rerio; RNA-Seq
The gfi1aa-/-gfi1ab-/- mutant and the gfi1aa-/-gfi1b-/-gfi1ab-/- mutant were generated from gfi1aa+/-gfi1ab-/- intercross embryos and gfi1aa-/-gfi1b-/-gfi1ab+/- intercross embryos by genotyping. Then, RNA from gfi1-related single, double, and triple mutants as well as WT embryos was extracted with TRIzol reagent. Sequencing libraries were generated using the NEBNext® UltraTM RNA Library Prep Kit for Illumina® RNA according to the manufacturer's instructions.