GSM6138639: p7l12D_2; Arabidopsis thaliana; RNA-Seq
For RNA isolation, plant material was harvested, frozen in liquid nitrogen and crushed in a TissueLyser (Retsch, model: MM400). Total RNA was extracted with the Direct-zol RNA Kit (Zymo Research, Irvine, USA) according to the manufacturer's protocol. RNA samples were quantified with a Nanodrop spectrophotometer (ThermoFisher Scientific, Langenselbold, Germany) and RNA integrity and quality was assessed by an Agilent 2100 Bioanalyzer (Agilent, Santa Clara, USA). Isolated RNA was stored at −80 °C until further use. Illumina Tru-Seq