Illumina HiSeq 2000 sequencing; RNA-seq on total RNA isolated from livers of 6-month DEN-treated Tid1-/- against WT mice
RNA-seq on total RNA isolated from livers of 6-month DEN-treated Tid1-/- against WT mice
Total RNA from tissues was prepared using TRIZOL reagent (Invitrogen) according to the manufacturer's instructions. cDNA synthesis was performed with SuperScript III reverse transcriptase (Invitrogen) and random hexamers.  Total RNA from tissues was prepared using TRIZOL reagent (Invitrogen) according to the manufacturer's instructions. cDNA synthesis was performed with SuperScript III reverse transcriptase (Invitrogen) and random hexamers.  Total RNA from tissues was prepared using TRIZOL reagent (Invitrogen) according to the manufacturer's instructions. cDNA synthesis was performed with SuperScript III reverse transcriptase (Invitrogen) and random hexamers.