GSM5772268: Torin2_H3K27me3_rep2; Arabidopsis thaliana; ChIP-Seq
For ChIP-seq, 1 g of seedlings were collected after removing all the liquid with Kimwipes. Plant materials were ground into powder and suspended in 25 mL of nuclear isolation buffer. The crude extracts were incubated with 1% formaldehyde at room temperature for 20 minutes and stopped by adding 3.4 mL1M glycine.   Histone-DNA complexes were isolated from  sonicated nuclei with antibody.  For-RNA-seq, whole seedlings from liquid medium or the aerial parts of plants from the agar plate were harvested and frozen in liquid nitrogen.  Total RNA was isolated from liquid nitrogen ground samples with the Trizol reagent (Invitrogen), DNase I treated (RQ1 RNase-free DNase I, Promega). The resulting RNA (0.5 μg) was used for preparing the library For ChIP-seq, NEBNext Ultra II DNA Library Prep Kit was used to make the library with 10 ng DNA. For enrich DNA fragments step, all the template and amplify 7 cycles. For-RNA-seq, NEBNext® Ultra II™ RNA Library Prep Kit for Illumina® using 500 ng of total RNA per library.  For enrich DNA fragments step, the templates were amplified for 8 cycles.