whole-exome sequencing (WES) for human Esophageal cancer
Sequencing libraries were prepared usingthe KAPA Hyper Prep kit (KAPA Biosystems) with an optimized manufacturer's protocol. In brief, 1gof genomic DNA, which was sheared into 350 bp fragments using the Covaris M278 instrument (Covaris), underwent end-repairing, A-tailing, and ligation with indexed sequencing adapterssequentially, followed by size selection for genomic DNA libraries using AgencourtAMPure XP beads (Beckman Coulter). Finally, libraries were amplified by PCR and purified using AgencourtAMPure XP beads.