GSM4981942: Terminal tracheal cells _ Pe1_DamPol_rep1; Drosophila melanogaster; OTHER
60 midguts with associated TTCs per condition per biological replicate were dissected in cold PBS and stored at -80°C. Genomic DNA was isolated, RNAseA treated and DpnI digested. DamID adaptors were ligated to DpnI digested DNA followed by DpnII digestion PCR amplification, sonication and removal of DamID adaptors (Marshall et al., 2016). Libraries were generated using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina.