GSM5834126: ARCS17; Rattus norvegicus; RNA-Seq
Total RNA was extracted using a Trizol-based method and quantified by Nanodrop. Small RNA deep sequencing and analysis was performed as previously described by the Human Molecular Genetic Center at the Medical College of Wisconsin (Kriegel et al, Physiol Genomics, 2013; PMID 24085797). Small RNA libraries were constructed from each sample using the Illumina TruSeq Stranded Total RNA. Samples were multiplexed on a 400 GB flow cell and pair-end sequenced using an Illumina HiSeq 2500.