GSM6198803: H3K56; Homo sapiens; Hi-C
For crosslinking, the cells suspension was treated with formaldehyde at a final concentration of 1% (H3K56/vol) and incubated for 10 min at room temperature, followed by quenching with 0.125 M glycine for 5 min at room temperature. After pelleted through 1,000 g for 5 min at 4 °C, the harvested cells were washed twice with ice cold PBS, aliquoted to 20 million aliquots per 1.5 ml Eppendorf tube, and stored at -80 °C for further delivery to Novogene Com., Beijing for Hi-C library generation. Hi-C library generation was conducted by Novogene Com., Beijing.