GSM5452910: LM2 single CTC at 10h [LM2_Singles_1000_11_060121]; Homo sapiens; RNA-Seq
Using the CellCelector, an automated single-cell picking system (ALS), single CTCs, CTC clusters and CTC-WBC clusters were collected and immediately transferred into individual tubes (Axygen, 321-032-501) containing 2.5 μl RLT Plus lysis buffer and 1U SUPERase IN RNA inhibitor (Invitrogen, AM2694). Samples were immediately frozen and kept at 800C until further processing. cDNA was amplifiead and libraries prepared according to the Smart-seq2 protocol.