GSM5385914: WT_PBS-1; Mus musculus; RNA-Seq
RNA was isolated using Ambion's mirVana kit (catalog #AM1561, Thermo Fisher) following the manufacturer's recommendations, and quantified on a Nanodrop 2000 (catalog # ND-2000, Thermo Fisher). All samples had 260/280 and 260/230 ratios between 1.8 and 2.1. RNA quality was further assessed with an RNA Nano Chip using a Bioanalyzer 2100 (Agilent). All samples had a RIN number of 8 or higher. Illumina TruSeq Stranded Total RNA Library Prep Kit (catalog # RS-122-2203, Illumina Inc). The mRNA expression was obtained by RNA-Seq libraries run on NextSeq 550 following the manufacturer's instructions.