DNA sequence of E.coli exposed to duloxetine(25mg/L) and chlorephenicol (4mg/L)
After E. coli strain K12 (MG 1655) was incubated at 37 C for 12h, 30 L of fresh cell suspension were added to 2.97 mL LB broth containing duloxetine (25 mg/L) and/or chloramphenicol (4 mg/L) to reach the final cell density of 106-107 CFU/mL. The E. coli cultures were incubated at 37 C for 24 h, then 30 L of the cultures were transferred into 2.97 mL fresh LB containing the corresponding concentration of duloxetine and/or chloramphenicol.