GSM5385170: WT TY1 rep1; Homo sapiens; ChIP-Seq
ChIP assays were performed on parental or RUNX1 mutation knock-in single clones of Jurkat cells using ChIP-IT High Sensitivity kit according to the manufacturer's protocol. Briefly, a total amount of 2e6 cells were treated with Complete Cell Fixative Solution for 15 minutes and Stop Solution for 5 minutes, followed by sonication. The following antibodies are used for ChIP assays: anti-HA (ab9110, Abcam), anti-TY1 (C15200054, Diagenode), anti- RUNX1 (ab23980), Normal Rabbit IgG (2729, Cell Signaling). Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303). Libraries were sequenced on the Illumina NovaSeq 6000 following the manufacturer's protocols.