GSM5335532: 3_S3_RNA-Seq; Mus musculus; RNA-Seq
RNA was extracted from FACS purified MNs dissected from E12.5 mouse embryo (10-20,000 cells/segment), using the Arcturus Picopure RNA isolation kit. For on-column DNase treatment, Turbo DNase was used (ambion, AM2238). Each sample used separated bar code for libraries. RNA quality and quantity were measured with an Agilent Picochip using a Bioanalyzer, all samples had quality scores between 9-10 RIN. For library preparation 10ng of total RNA was used to generate cDNA, which was amplified with SMARTer Stranded RNA-Seq kit. 100ng of cDNA were used as input to prepare the libraries (Takara, #634837), and amplified by 10 PCR cycles. Samples were run in four 50-nucleotide paired-end read rapid run flow cell lanes with the Illumina HiSeq 4000 sequencer.