GSM5833797: Dt47.day4.AZA.0; Homo sapiens; OTHER
On the day of the experiment, DNA was extracted from frozen pellets with an Allprep DNA/RNA Mini Kit (Qiagen). Bisulfite conversion was performed on 1 μg DNA with an Epitect kit (Qiagen), and a 457bp region of the HBG1/2 promoter region was amplified by nested PCR as described previously (Mehta et al. 2015). PCR amplicons were sent for Illumina Amplicon next-generation sequencing (Genewiz), yielding 2x250 bp reads.