GSM5692980: LCuA rep1; Thalassiosira oceanica CCMP1005; RNA-Seq
Total RNA was isolated from the filtered cells (~70 million cells) using a TRIzol Plus RNA Purification Kit (Thermo Fisher Scientific) with an on-column DNase (TURBO DNase, Thermo Fisher Scientific) digestion step to remove genomic DNA, as per the manufacturer's recommendation. Messenger RNA was isolated from 1 μg high-quality (RIN≥6.5) total RNA using oligo-dT attached magnetic beads and cDNA sequencing libraries were constructed with fragmented mRNA by a TruSeqTM RNA Sample Preparation Kit (Illumina, CA, USA), as per the manufacturer's recommendation.