GSM5385865: MDA-MB-453 H3K27ac ChIP Veh Consensus; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 m prior to quenching. Chromatin was prepared and subsequently sheared by the Diagenode BioRuptor Plus for 10 cycles (30 sec on, 30 sec off) on ice. Sheared chromatin was immunoprecipitated with 5 µg GATA3, 5 µg AR, or 2 µg H3K27ac antibody overnight, after coupling to Protein A Dynabeads (10002D). DNA was purified using phenol:chloroform:isoamyl alcohol extraction. Libraries were prepared according to manufacturer's instructions accompanying the Qiagen Ultralow Input Library Kit (Part #180495). ChIP-Seq; Illumina NextSeq 500 (High Output v2) single-end 75 bp reads.