GSM5452701: Untreated Control_3; [Candida] auris; RNA-Seq
Freeze- lyophilized cells were processed using TRISOL reagent (Invitrogen, Austria) reagent and glass beads. RNA concentration and purity were determined using NanoDrop (ThermoFisher Scientific, Waltham, MA USA) and capillary electrophoresis. The quality of RNA was determined with the Eukaryotic Total RNA Nano kit (Agilent, USA) using Agilent Bioanalyzer. RNA-Seq libraries were prepared from total RNA using NEBNext Ultra II RNA Sample Preparation kit (NEB, USA) according to the manufacturer's protocol. The single read 75 bp long sequencing reads were generated on Illumina NextSeq500 instrument. Approximately 18-22 million reads per samples were generated.