GSM6340412: Lis46_late_naive_rep2; Homo sapiens; RNA-Seq
RNA was extracted using mirVana miRNA Isolation Kit (Life Technologies, Inc.), following the manufacturers' instructions. RNA quality was assessed using a BioAnalyzer 2100 (Agilent Technologies, Inc.). All samples had a RIN greater than 8.5. RNA-seq libraries were constructed in triplicate using the KAPA mRNA HyperPrep Kit (Roche) with 500 ng of input RNA according to the manufacturers instructions.