Biofilm forming clinical Isolates of Candida tropicalis
Whole genome sequencing (WGS) libraries were prepared with Illumina-compatible SureSelectQXT whole genome library prep kit. About 30ng of Qubit quantified genomic DNA was fragmented and adapter-tagged using Sure Select QXT Enzyme. Fragmented and adapter-tagged DNA was purified with HighPrep PCR beads and then amplified and indexed in a 6 cycle PCR reaction (68C for 2 min, denaturation at 98C for 30 sec, cycling (98C for 30sec, 56C for 30sec and 72C for 60s). The final PCR product was purified with HighPrep beads, followed by library quality control check.  The Illumina-compatible sequencing library were pooled and quantified by Qubit fluorometer and its fragment size distribution was analyzed on Agilent TapeStation.