DNA-Seq of stickleback virus
The cell culture supernatants were harvested at third passage by clarifying at 3000xg for 20 minutes. The total DNA was purified by DNeasy blood and tissue kit (Qiagen). Libraries were constructed from the DNA extracts using Nextera XT DNA kit (Illumina), and genome sequencing was performed using a V3 chemistry 600-cycle kit on a MiSeq platform (Illumina)