GSM5381515: Day 7 - Diff. Medium rep3; Homo sapiens; RNA-Seq
RNA-Seq: Cells were harvested, frozen and sent to Active Motif Inc. platform for library preparation and sequencing. Illumina TruSeq Stranded mRNA Sample Preparation Kit was used for library preparation. ChIP-Seq: Cells were harvested, frozen and sent to Broad Institute's Genomics Services platform for the ChIP data and to Active Motif Inc. platform for the input data. H3K27ac antibodies (Acetyl-Histone H3 K27 (D5E4) XR Rabbit mAB, Cell Signaling Technologies were used for precipitation. Library preparation and Sequencing for the ChIP-data were performed in a multiplexed system on a 96-well plate. RNA libraries were prepared for sequencing using standard Illumina protocols