RNA seq of MSM DRKIN
Total 100 ng of ribodepleted RNA was utilized and converted into the final library using Truseq stranded RNA library preparation kit following manufacturer's protocol (Illumina, USA). Minimum 8 million high-quality paired-end reads were generated from each sample. Raw reads were trimmed for adapter removal, and high-quality reads were utilized for the differential gene expression calculations using the DESeq2 tool.