Kombucha Bacteria Black Tea 15 days fermantaion liofilizated
DNA extraction, purification, and amplification were performed according to102 Ruiz et al. (2000) with some modifications such as: (i) use of GRS Genomic DNA and103 PCR & Gel Band Purification (GRiSP Research Solutions) for extraction and purification104 DNA; (ii) use of primers 16S-F (5-GCTGGCGGCATGCTTAACACAT-3) and 16S-R105 (5-GGAGGTGATCCAGCCGCAGGT-3); (iii) for the amplification, the samples were106 incubated for 5 min at 94 C to denature the target DNA, and cycled 35 times at 94 C107 for 1 min, 58 C for 1 min, and 72 C for 1 min.; (iv) PCR amplification product was not108 digested with restriction endonucleases.