GSM5833462: 5_15T; Pseudomonas aeruginosa PAO1; RNA-Seq
200μL of Trizol was added to the channel. Trizol was collected and stored at -80°C for RNA isolation. Following a 5 min incubation at room temperature, 0.2 mL of chloroform was added and the samples were shaken vigorously for 1 min. Phases were separated by centrifugation (12,000 g, 5 min, 4°C) and the aqueous phase was combined with 0.6 mL of 70% ethanol and transferred to an RNeasy mini column (Qiagen). RNA was purified as per manufactures protocols Libraries were constructed using the ScriptSeq Complete Kit (Illumina) following the Bacteria-Low Input protocol.