GSM4980479: Tbx1CrklWtR03; Mus musculus; RNA-Seq
10X: live Ter119-CD45-EpCAM- (developmental) or Ter119-CD45- (Tbx1Crkl) thymic stromal cells were sorted for 10X Genomics 3' gene expression. For the Tbx1Crkl dataset, cells were pre-fixed with RNA Protect. RNA-seq: Live CD45- thymic stromal cells were sorted for ultra-low input RNA-seq. 10X GEX: libraries were prepared as per manufacturer's protocol for 3' gene expression libraries. RNA-seq: ultra low RNA-seq protocol (equivalent to Smarter-seq)