GSM5381426: ΔwhiB4 infected kidney_rep3; Danio rerio; RNA-Seq
For RNA extraction of zebrafish kidneys, the samples were removed from RNA-Be-Locker A Reagent by centrifugation and added with 1ml TRIZOL for lysis. The insoluble tissues were removed by centrifugation and supernatants were collected and added with equal volume ethanol. The RNA was then extracted using the Tianmo#TR205-200 kit. RNA libraries were prepared for sequencing using standard Illumina protocols