16S rRNA gene-based analysis of the bacterial community of sample 5 from Faecal material with DNA extraction method QSB of cow A
Metagenomic DNA was extracted from the Faecal fraction of Cow A, using the Qiagen Soil kit Bead beating extraction method. The V3-V4 region of 16S rRNA genes was amplified. All samples were amplified in triplicate, purified and pooled in equal amounts. Sequences were determined using an Illumina MiSeq sequencer.