ZymoBIOMICS Microbial Community Standard (cat # D6300)
Total DNA was sent to the Integrated Microbiome Resource at Dalhousie University (Halifax, Nova Scotia), for PCR amplification and sequencing. Sequencing libraries were prepared by targeting the V4-V5 regions of the 16S rRNA gene for amplification using the the 515F (GTGYCAGCMGCCGCGGTAA) and 926R (CCGYCAATTYMTTTRAGTTT) primer set.