GSM6198808: H3K56bhb_rep2; Homo sapiens; ATAC-seq
50,000 cells were resuspended in 50 ml of cold ATAC-seq resuspension buffer (RSB; 10 mM Tris-HCl pH 7.4, 10 mM NaCl, and 3 mM MgCl2) with 0.1% NP40, 0.1% Tween-20, and 0.01% Digitonin, and incubated on ice for 3 minutes. After centrifuging at 500 rcf for 5 min at 4 °C, the lysis was washed out with 1 ml cold ATAC-RSB containing 0.1% Tween-20. Cell pellet was resuspended in 50 ml of transposition mixture (25 ml 2x TD buffer, 2.5 ml transposase (100nM final), 16.5 ml PBS, 0.5 ml 1% digitonin, 0.5 ml 10% Tween-20, 5 ml H2O) and incubated at 37°C for 30 minutes at 1000 rpm. Libraries from the purified DNA were prepared using Vazyme TruePrepTM DNA Library Prep Kit V2 for Illumina® according to the manufacturer-supplied protocol, the 200~700 bp length amplified DNA were size selected using the SPRIselect double side size selection kit (Beckman Coulter), analyzed with Qubit assays and Fragment Analyzer, and finally sequenced on the HiSeq Xten PE150 sequencing platform (Novogene, Beijing).