GSM5453393: Gcn4 G-SELEX Round 3 Replicate 2; Saccharomyces cerevisiae; OTHER
The sonicated DNA was gel purified to isolate fragments < 200 base pairs in length Gel-purified DNA fragments were ligated to the Illumina paired-end adaptor and amplified with InPE 1.0 and InPE 2.0 (Illumina) primers as described in kits from New England Biolabs. G-SELEX - incubation with protein of interest, followed by washing and elution of DNA bound to protein.  Bound DNA is PCR amplified and subjected to 2 subsequent incubation rounds (3 total) before Illumina paired-end sequencing