RNAseq data of Neurospheres-Derived Cells from Human Olfactory Epithelium
RNA was isolated from the cells using RNeasy column kits (Qiagen), then treated with DNase I and purified using the CleanRNA Standard kit (Evrogen). cDNA libraries were constructed with 500 ng of total RNA using NEBNext Ultra II Directional RNA Library Prep with NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) and barcoded with dual index primers for pooled sequencing.