pond water microbiome
We conducted high throughput sequencing of the 16S rRNA gene from whole guts of lab reared green frog tadpoles exposed to three temperature conditions (14C, 22C, 28C) and two microbial colonization treatment groups (colonized, depleted). We also took samples from natural pond water and pond water stored in the lab (which tadpoles were housed in). Total DNA was extracted from gut and water samples using a QIAGEN Power Fecal Pro DNA Isolation Kit. PCR and sequencing was conducted by the DNA services facility at University of Illinois at Chicago for experiment 1 and the Microbial analysis resources and services facility at the University of Connecticut for experiment 2.