Illumina NovaSeq 6000 paired end sequencing; mRNA-seq of DND-41 T-cell acute lymphoblastic leukemia cell line upon inhibition of hsa-miR-363-3p
mRNA-seq of DND-41 T-cell acute lymphoblastic leukemia cell line upon inhibition of hsa-miR-363-3p
3 million cultured DND-41 T-ALL cells were harvested and rinsed twice with phosphate buffered saline. Total RNA was isolated using miRNeasy Kit (Qiagen). RNA isolates were DNase treated and purified with use of RNA Clean and Concentrator Kit (Zymo Research).  Libraries were generated with Illumina TruSeq Stranded mRNA Library Prep Kit. Standard fragmentation conditions were used (94â€™C for 8 minutes).