GSM4980327: Hela-3; Homo sapiens; miRNA-Seq
The supernatant was collected to isolate exosomes and extract a total of 1 μg RNA as input material for RNA sample preparation. The library was quality and quantitated in two methods: check the distribution of the fragments size using the Agilent 2100 bioanalyzer, and quantify the library using real-time quantitative PCR (Q-PCR) (TaqMan Probe). The final ligation PCR products were sequenced using the BGISEQ-500 platform.