GSM5574488: NAI_rep2_pre_ANKRD52_DNAtemplate; Homo sapiens; OTHER
RNAs in the reactions were extracted by TRIZOL according to the manufacturer's protocol, followed by DNase I digestion and phenol/chloroform extraction. Next, recovered RNAs were used for reverse transcription using SuperScript II (Invitrogen) and specific primers for circular or linear ankrd52 RNAs. Finally, nest PCRs were done to amplify circular or linear ankrd52 DNAs using primers for library preparation and deep sequencing.