GSM5834162: OP.36; Homo sapiens; RNA-Seq
Amygdala samples from humans with OUD and unaffected controls were processed for total RNA isolation using the mirVana miRNA Isolation Kit (ThermoFisher Scientific, San Diego, CA, USA) and Zymo purification kit (Zymo Research, Irvine, CA, USA). Libraries were prepared with the KAPA RNA HyperPrep Kit with RiboErase (Roche, Basel, Switzerland), which removes both cytoplasmic and mitochondrial ribosomal RNA (rRNA) species and uses random priming in cDNA synthesis, according to the manufacturer's protocol. The rRNA-depleted libraries were subsequently sequenced on an Illumina Novaseq sequencer at 100-million-read target coverage (100 bp paired-end reads) at the Yale Center for Genome Analysis facility.