GSM5452202: PHF13; Homo sapiens; ChIP-Seq
Panc-1 cells in one 15 cm plate were crosslinked sequentially as the followings: for 40 minutes in 5 mM EGS (Thermo Fisher Scientific) at room temperature, for 40 minutes in 1% paraformaldehyde (Sigma) at 4 ℃, then for 10 min in 1% formaldehyde (Sigma). The chromosome was fragmented to the fragment range of 200-400 bp in the Bioruptor® Pico via sonicating 5 cycles. Then, according to the manufacturer's protocol, ChIP was further processed using the ChIP-IT High Sensitivity Chromatin Immunoprecipitation kit. The VAHTS Universal DNA Library Prep Kit for Illumina V3 (ND607)  was used with 5 ng of ChIP DNA of each sample for the construction of sequencing libraries.