16S amplicon ofAN2405
Bacterial 16S rRNA gene V4-V5 hypervariable regions were amplified using primers 515F (5-GTGCCA GCM GCC GCG GTA A-3) and 907R (5-CCG TCA ATT CCT TTG AGT TT-3) combined with adapter sequences and barcode sequences.  Purified amplicons were sequenced HiSeq2500