GSM5906518: mp44_CD4_DP_2; Homo sapiens; RNA-Seq
RNA from fresh-frozen tumor fragments was isolated using TRIzol reagent(Invitrogen). RNA from RLT-lysed cells was extracted using the RNeasy mini kit (Qiagen) according to the manufacturer's protocol. RNA concentration was measured with the Qubit RNA HS Assay Kit (Thermo Fisher Scientific, US). No more than 500 ng of total RNA was used for cDNA synthesis. cDNA libraries were generated using the Human RNA TCR Multiplex kit (MiLaboratories), according to the manufacturer's protocol. other: targeted RNA-seq (TCR amplicon seq)