GSM5385910: GAR15-13_Input; Homo sapiens; ChIP-Seq
tumors were cut into 30 µm slices on a cryostat and fixed with 2 mM disuccinimidyl glutarate for 25 m followed by 1% formaldehyde for 20 m prior to quenching. Chromatin was prepared and subsequently sheared by the Diagenode BioRuptor Pico for 5 cycles (30 sec on, 30 sec off) on ice. Sheared chromatin was immunoprecipitated with 5 µg GATA3 antibody overnight, after coupling to Protein A Dynabeads (10002D). DNA was purified using phenol:chloroform:isoamyl alcohol extraction. Libraries were prepared according to manufacturer's instructions accompanying the Qiagen Ultralow Input Library Kit (Part #180495). ChIP-Seq; Illumina NextSeq 500 (High Output v2) single-end 75 bp reads.