GSM5693090: S-RhoBTB1.AngII_rep4; Mus musculus; RNA-Seq
Aortae were disscted, cleaned and snap frozen in liquid nitrogen. Tissue were lysed in Trizol using a bead mill homogenizer. Total RNA were extracted following the instructions provided by the Invitrogen Purelink kit. RNA was quantifed by fluorimetirc methods (Qubit, Invitrogen) and quality assessed by Fragment Analysis (Agilent).  Library preparation was initiated with 500ng of RNA and completed following manufacture's recommendations for the TruSeq stranded mRNA kit (Illumina).  Final libraries were quantified by qPCR (Kapa Library Quantification Kit, Kapa Biosystems) and fragment size observed with high sensitivity DNA fragment analysis (Agilent).  Paired end sequencing was completed on the NovaSeq 6000 at 2x100bp reads, targeting 50 million reads per sample.