Total RNA-Seq in HSV-1 infected human fibroblasts
MRC5 cells were mock-infected or infected with d109, 5dl1.2, n12, n199, n212, hp66 or wildtype HSV-1 (KOS) at an MOI of 10 PFU/cell for twelve hours. Total (RiboMinus) RNA-Seq libraries were generated. Illumina sequencing was performed using NextSeq550 to generate 75 PE reads, adaptors are Illumina TruSeq 6 base.