GSM5213546: Serially Blood Passaged Infection Day 2 Rep 1; Mus musculus; RNA-Seq
Splenic Material was extracted using the RiboPure™ RNA extraction kit (Invitrogen, ThermoFisher) according to the manufacturer's instructions. The quantity and quality of the RNA samples were verified using NanoDrop 1000 Spectrophotometer (Thermo Fisher Scientific) and Bioanalyser. RNA was used for cDNA library preparation using the KAPA RNA Hyperprep with Riboerase kit. 100bp PE reads to depth of 25 million reads per sample.