RNA-Seq of THIP-1 cells: withoutTreponema Pallidum stimulation
Total RNA from the two groups was extracted using TRIzol reagent (Invitrogen). Whole-cell total RNA was isolated using TRIzol reagent (Invitrogen) and quantified using a Qubit RNA Assay Kit on a Qubit 2.0 Fluorometer (Life Technologies, CA, USA). RNA purity was assessed using a NanoPhotometer spectrophotometer (IMPLEN, CA, USA). A cDNA library was constructed using the NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA). Sequencing was performed on an Illumina NovaSeq 6000 platform at Novogene (Beijing, China). High-quality reads were mapped to the human reference genome (hg38) using HISAT2. Differential expression analysis of the two groups was performed using the DESeq2 package in R (1.10.1). Genes with an adjusted P-value <0.05 were considered differentially expressed. Sequencing was carried out with 3 independent biological replicates.