RNAseq of Arabidopsis thaliana: Heat stress
RNA was isolated using Direct-zol RNA MiniPrep Plus (Zymo Research, CA, USA) according to the manufacturers instructions. Quality was assessed using a Bioanalyzer (Agilent Technologies, Waldbrann, Germany) and quantity was determined using Qubit (Invitrogen, Life Technologies). High-quality RNA (3 g) was used to prepare libraries for sequencing. RNA-sequencing 150-bp paired-end (PE) libraries were prepared from mRNA-enriched samples, using the NEB Next Ultra DNA Library Prep Kit for Illumina sequencing (New England Biolabs), according to the manufacturers instructions. A total of 12 samples were pooled in one lane of the flow cell.