GSM5726904: SH1000 20 min rep 2; Staphylococcus aureus; RNA-Seq
The total RNA was extracted using organic extraction with TRIzol. Genomic DNA was removed using a Turbo DNA-free kit. The rrRNA depletion was performed using a RiboMinus Bacteria 2.0 Transcriptome Isolation Kit according to the manufacturer's instructions. Sequencing libraries were prepared using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina kit, following the standard protocol.