Illumina HiSeq 2500 sequencing; Bulk RNA-sequencing of FACS isolated human fetal lung, intestine, and kidney endothelial cells
Bulk RNA-sequencing of FACS isolated human fetal lung, intestine, and kidney endothelial cells
Cultured HUVECs were dissociated using accutase and collected. Cells were pelleted, immediately snap frozen, and stored at -80 oC prior to RNA isolation. RNA was isolated from snap frozen cell pellets using the RNeasy Mirco Kit (74004, Qiagen), according to manufacturer's guidelines. cDNA libraries were prepared using the SMARTer Stranded Total RNA-Seq Kit v2- Pico Input (634412, Takara) by the University of Michigan Advanced Genomics Core according to manufacturer's guidelines.