387-Tumor-D1-RESUB_R1_001.fastq.gz
Tumor organoids were derived from Smad4KO BRAFV600E/+ Villin-CreERT2 and Msh2KO Smad4KO BRAFV600E/+Villin-CreERT2 intestinal tumors. DNA was extracted from tumor organoids and matched tails using DNeasy Blood & Tissue Kit (QIAGEN). Samples were submitted for Whole Exome Sequencing (WES) (GENEWIZ). Tumor and tail raw files were aligned using BWA-MEM (0.7.17) to the mouse reference genome (mm10) and processed using GATK Best Practices and mouse analysis pipelines. Filtered calls were annotated using ENSEMBL VEP.