GSM5574503: hMSC-hTERT20_Day7_R2; Homo sapiens; RNA-Seq
Total RNA was isolated from Trizol with DNase treatment using the Direct-zol RNA mini-prep kit (Zymo Research #R2050). RNA libraries were prepared for sequencing using TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero (Illumina) using standard protocols. Barcoded libraries were loaded onto an Illumina HiSeq 1500 and single-end 100-base (SE100) sequencing was performed at the The Vermont Integrative Genomics Resource (VIGR).