GSM4855903: prp2_B; Saccharomyces cerevisiae; RNA-Seq
Temperature shifted cells were collected via vacuum filtration, flash frozen in liquid nitrogen, and pellets were stored at -80˚C. RNA was extracted using Phenol:Chloroform extraction as presented in Xu et al. Nature Methods 2019 Libraries were prepared using MPE-seq as presented in Xu et al. Nature Methods 2019 using 10ug RNA input