GSM4981314: CD45+ Jak2V617F Gsdmd-/-, scRNA-seq; Mus musculus; RNA-Seq
Aortas were mechanically cleaned, then diced and digested with Liberase, hyaluronidase, and Dnase in media for 45 minutes at 37C. Cells were then washed, blocked with FC block, and stained for DAPI and CD45. Samples were then sorted using flow cytometry (DAPI negative, CD45 postive) and subjected to scRNA-Seq pipeline Libraries were prepared using the 10x Genomics Chromium Single Cell 3' Reagent Kits v2 according to manufacturer's instructions. 5000 cells and 200M reads per sample were targeted. 12 cycles for cDNA amplification and 12 cycles for sample index PCR were used.