Illumina HiSeq 2000 paired end sequencing; Ethylene perception modulates tomato pollen tube growth by altering calcium signaling and cell wall remodeling
Ethylene perception modulates tomato pollen tube growth by altering calcium signaling and cell wall remodeling
pollen tube after 4 h of germination The germination medium contained 300 mM sucrose, 2 mM boric acid, 2 mM calcium nitrate, 2 mM magnesium sulfate, 1 mM potassium nitrate and 0,5 % (w/v) agarose, as described Twenty mg of the pollen powder were taken for total RNA extraction. The total RNA was isolated from the sample using the ReliaPrep™ RNA Tissue Miniprep System kit (Promega, Charbonnières-les-Bains, France) and treated with DNase I (Invitrogen, Illkirch, France) to remove DNA. cDNA