GSM4980056: CUT&Tag BSA condition 3; Mus musculus; OTHER
CUT&run was performed according to https://dx.doi.org/10.17504/protocols.io.zcpf2vn ; Cut&tag was performed according to https://dx.doi.org/10.17504/protocols.io.bcuhiwt6 Briefly, fresh nuclei were incubater with primary, secondary antibody, then proteinA-Tn5 fusion and Tn5 was activated by Mg2+. Nuclei were lysed using SDS and proteinase K, DNA extracted using Zymo DNA clean&concentrator-5 kit, and eluted DNA used as input to PCR with sample-specific barcoded primers. Cut&run libraries were prepared using Kapa hyper prep kiy (Kapa biosystems, 7962363001); CUT&Tag libraries were prepared by tagmentation