GSM5216344: Non-hematopoietic cells from Flk2-cre+ Cxcr4fl/+ mouse [CTL-CXCR4]; Mus musculus; RNA-Seq
Bone marrow stromal cells and bone surface cells were isolated. CD45- Ter119- Lineage (B220, CD19, CD11b, Gr1, CD3e)- CD31- CD144- CD71- cells were sorted by the BD FACS Aria II machine into DMEM with 20% FBS. scRNA-Seq library was generated using Chromium Single Cell 3' Reagent Kits v3 (10x Genomics) following the manufacturer's protocol.