Paired-end Illumina sequencing of Salmonella enterica strain 2020-004 isolated from contaminated flax seeds
Strain grown 24h at 35C on TSA plate and 10uL loopful of cells was resuspended in 800uL Zymo DNA/RNA Shield. DNA extracted using Zymo Quick-HMW kit and RNAseA treatment, and prepared with NexteraXT library prep; paired-end (2x300bp) sequencing using MiSeq and v3 chemistry.