GSM5452215: heart_HL7W-32; Mus musculus; RNA-Seq
The mice were euthanized by intraperitoneal injection of pentobarbital at 5th and 7th week post the dual intervention. Heart tissues were then removed and frozen in liquid nitrogen.Total RNA of mouse heart tissues was extracted using Trizol, according to manufacturer's procedure, quantified and quality controlled using a 2100 Bioanalyzer (Agilent). RNA-seq libraries were prepared using the NEBNext UltraTM RNA Library Prep Kit for Illumina® .