GSM6198443: Human_paraffin-embedded_placental_tissue_Sample 85; Homo sapiens; RNA-Seq
Placenta samples were formalin-fixed and paraffin-embedded; five 20-micron sections of each placenta sample were subjected to RNA extraction using the RNAstormTM kit (Cell Data Sciences, Fremont, CA, USA) as per the manufacturer's instructions. Total RNA was processed for high-throughput sequencing using the QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen, Greenland, NH, USA). Following mRNA generation, fragmentation, and cDNA synthesis, adaptor barcodes were ligated and final cDNA libraries were amplified by PCR and assessed for quality and concentration.