GSM5386799: day 60 2D monolayer PSC Skeletal myogenic differentiated cells-rep 1; Homo sapiens; RNA-Seq
RNA was purified using Trizol (Thermo Fisher Scientific) according to the manufacturer's instructions. RNA quality was ensured with the Fragment Analyzer from Advanced Analytical by using the standard sensitivity RNA Analysis Kit (DNF-471). RNA-seq libraries were prepared using a modified strand-specific, massively parallel cDNA sequencing (RNA-Seq) protocol from Illumina, the TruSeqStranded Total RNA. Libraries were sequenced on a HiSeq4000 platform (Illumina) generating 50 bp single end reads (30-40 Mio reads/sample).