GSM5386023: Ex +3h  ZT0 3; Mus musculus; RNA-Seq
The glycolytic quadriceps and gastrocnemius muscles were quickly snap frozen in liquid nitrogen and stored at - 80°C. Total RNA from muscle tissues was extracted using a hybrid method combining TRI-Reagent (Sigma-Aldrich T9424) and RNeasy Mini Kit (QIAGEN 74104). RNA quantity and purity were measured with a NanoDrop OneC (ThermoFisher Scientific). RNA quality was determined on the Bioanalyzer instrument (Agilent Technologies, Santa Clara, CA, USA) using the RNA 6000 Nano Chip (Agilent, Cat# 5067-1511) and quantified by Spectrophotometry using the NanoDrop ND-1000 Instrument (NanoDrop Technologies, Wilmington, DE, USA). Library preparation was performed with 1µg total RNA using the TruSeq Stranded mRNA Library Prep Kit High Throughput (Cat# RS-122-2103, Illumina, San Diego, CA, USA).