pThr interactome of 14-3-3 beta from HI-P
100-200 ng of plasmid DNA was amplified in four reactions using a standardized set of OLS amplification primers (forward: TCTGGGTCGACTGGTGGTACC, reverse: CGTACCATGTAGCTTAATCAGCTGTTAAAGCTT) with Q5 polymerase (NEB #M0491). PCR cycle followed a 98C denaturation for 30s, 15 cycles of 98C for 15s, 58C for 30s, and 72C for 30s, with a final extension of 72C for 2 minutes. Samples were then cleaned and concentrated with a PCR purification kit (Qiagen #28104) to one 50 uL sample. Samples were then further concentrated with a BluePippin (Sage Science) selecting for samples between 100-200 bp.  Samples were then cleaned and concentrated with a PCR purification kit (Qiagen #28104) to a 40 L sample. 35 L of sample at a concentration of 30-50 ng/uL was then sent to Massachusetts General Hospital Center for Computational & Integrative Biology DNA Core for CRISPR amplicon next-generation sequencing.