16s-Seq of homo sapiens liver
DNA from liver biopsies were extracted and Real-time polymerase chain reaction (qPCR) amplification was performed using 16S universal primers targeting the hypervariable V3-V4 region of the bacterial 16S ribosomal gene with a protocol carefully designed to minimize any risk of contamination between samples or from the experimenters, environment. The quality and quantity of extracted nucleic acids were controlled by gel electrophoresis and absorbance spectroscopy using a NanoDrop 2000 UV spectrophotometer. The qPCR was performed on a ViiA 7 PCR system using Sybr Green technology. The microbial populations based on rDNA present in liver samples were determined using next-generation high-throughput sequenced using the Illumina-MiSeq technology.