eDNA metabarcoding of marine sediment: 18S sequencing
DNA was extracted from 10 g sediment of each sample by using the PowerMax Soil DNA Isolation Kit (Mo Bio laboratories, Inc., USA) according to the manufacturers instructions. The primer set, 1380F (5'-CCCTGCCHTTTGTACACAC-3') and 1510R (5'-CCTTCYGCAGGTTCACCTAC-3'), targeting the V9 region of the 18S rRNA gene was used. PCR amplification was conducted in a volume of 20 L with three replicates. The high-throughput sequencing was performed on the Illumina Hiseq 2500 platform (PE250) by Novogene Co., Ltd (Beijing, China).