GSM5833674: HC-067; Homo sapiens; RNA-Seq
2.5ml of whole blood was collected from each participant into PAXgene Blood RNA tubes (PreAnalytiX GmbH, Switzerland). RNA was isolated from each samples using the RiboPure RNA purification kit (Ambion ThermoFisher; Waltham, MA, USA) following the manufacturer's protocol. Quantification and quality assessment of total RNA was performed using an Agilent 2100 Bioanalyzer (Santa Clara, CA, USA). Poly-adenylated RNA was captured using the NEBNext Poly(A) mRNA Magnetic Isolation Module (catalogue no.: E7409L, NEB; Ipswich, MA, USA). Strand-specific cDNA libraries were generated from poly-adenylated RNA using the KAPA Stranded RNA-Seq Library Preparation Kit (cat. no.: 07277253001, Roche; Basel, Switzerland).