Short-read sequencing of human HCT pediatric patient BSI isolate
BSI isolates from HCT patients were placed into an agar slant.  Bacteria were then cultured from the agar slant into liquid LB without antibiotics and grown at 37C until confluent.  Bacteria were spun down to a pellet and DNA was extracted from samples using a mechanical bead beating approach and the QIAamp Fast DNA Stool Mini Kit. Sequencing libraries were prepared with the Nextera Flex or Nextera XT kit and sequenced on an Illumina HiSeq 4000.