Illumina HiSeq 2000 paired end sequencing; Global transcriptome of CFA/I expressing ETEC H10407 under in vitro growth and water stress
Global transcriptome of CFA/I expressing ETEC H10407 under in vitro growth and water stress
CFA/I-positive strain H10407 was grown under different in vitro conditions to reach the early exponential growth phase (OD600 = 0.4). For water survival, H10407 was suspended in a water to a final concentration of 1 × 10(6) cfu/mL and incubated at room temperature without shaking for 48 h . RNA was extracted using the RNeasy Mini Kit (Qiagen, Hilden, Germany). Ribosomal RNA was depleted using Invitrogen's RiboMinus Prokaryotic Kit (Invitrogen Inc.) and contaminating DNA was removed using RNase-free DNase (Invitrogen Inc.). The cDNA libraries were prepared using Illumina TruSeq RNA sample preparation kit