RNA-seq of mus musculus: microvessels
Total RNA was isolated using the Trizol/chloroform extraction method. RNA concentration, purity, and integrity was evaluated using Agarose Gel Electrophoresis and Agilent Bioanalyzer RNA 6000 Kit. Only RNA samples with RNA integrity number >8 were used for library preparation. Total RNA-Seq library construction used the Illumine NovaSeq 600 platform. Total RNA was rRNA depleted followed by enzymatic fragmentation, cDNA synthesis, and double-stranded cDNA purification. Each RNA-Seq group contained three biological replicates.