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Samples were cross-linked in 1.8% formaldehyde and then were sonicated to produce DNA fragments with an average size of ~500 bp. Sonicated lysates were added to Dynabead protein G coupled with -Yorkie antibodies and rotated overnight at 4 C. The chromatin samples were reverse cross-linked at 65 C for 16 hours. Immunoprecipitated DNA was prepared for next generation sequencing using the Epicenter Nextera DNA Sample Preparation Kit. Library construction was performed using the High Molecular Weight tagmentation buffer, and tagmented DNA was amplified using 14 cycles of PCR. The libraries were then sequenced on a Novaseq according to manufacturers standard protocols.