GSM5334807: F-12; Homo sapiens; miRNA-Seq
Tissue was obtained from chromosomally normal (46XX / 46XY) fetuses in their second trimester (17-24 weeks gestation), after elective terminations for medical reasons. TRIzol reagent (Life Technologies, Carlsbad, CA, USA/ThermoFisher, MA) was used to isolate total RNA from frozen tissue samples. Full description of library construction and sequencing are as previously described [TCGA: Comprehensive molecular portraits of human breast tumours. Nature 2012, 490(7418):61-70].