GSM5573784: ATAC-Seq Th17_24h_LNA4_replicate2_LANE1; Homo sapiens; ATAC-seq
MIAT deficient and sufficient cells were differentiated under Th17 conditions for 72 h in three biological replicates. Nuclei were isolated as described earlier (Buenrostro et al., 2013, 2015) except that the cells were lysed by Digitonin (Promega, cat no. G9441) to avoid mitochondrial DNA contamination. Nextera DNA library preparation kit with i7 and i5 indices (15028212) and AMPURE-XP beads (B46053, Beckman Coulter) were used to prepare and purify the libraries, following the manufacturer's instructions. 18 samples pooled into one pool and sequenced on 2 lanes with 2x50 bp read length. Sequencing was performed at the Finnish Functional Genomics Centre using the NovaSeq 6000 S1 Next-Generation Sequencing platform.