GSM5693354: C130 rRNA-free RNA; Homo sapiens; RNA-Seq
(rRNA-free RNA) Total RNA was extracted from cell or animal tissue by Trizol reagent(Invitrogen) separately. The RNA quality was checked by Bioanalyzer 2200(Aligent) and kept at -80℃.The RNA with RIN >8.0 is right for cDNA library construction. (miRNA) Total RNA was extracted from cell or animal tissue by Trizol reagent(Invitrogen) separately. The RNA quality was checked by Bioanalyzer 2200(Aligent) and kept at -80℃.The RNA with RIN >8.0 is right for miRNA purification. miRNA was purified by miRNeasy Mini Kit(Qiagen) and the purification result was validated by Gel electrophoresis. (rRNA-free RNA) RNA libraries were prepared for sequencing using standard Illumina protocols (miRNA) RNA libraries were prepared for sequencing using standard Illumina protocols