RNAseq of HDAC6KO mice : Bone Marrow Mast Cells
The isolated total RNA was processed for preparing mRNA sequencing library using the Illumina TruSeq Stranded mRNA Sample Preparation kit (Illumina) according to the manufacturer's protocol. Quality and size of libraries were assessed using Agilent 2100 bioanalyzer DNA kit (Agilent). All libraries were quantified by qPCR using CFX96 Real Time System (Biorad) and sequenced on the NextSeq500 sequencers (Illumina) with a paired-end 75bp plus single