GSM5333535: PD1mid TIL H3K4me3 2; Mus musculus; OTHER
CD8 T cells were isolated from tumors by surgical excision followed by flow sorting.CUT&RUN was performed as previously described (Skene et al., 2018). ). Libraries were prepared using NEBNext Multiplex Oligos for Illumina and either the sparQ DNA Library Prep Kit ) or NEBNext Ultra II DNA Library Prep Kit for Illumina.  Cluster generation and 75-bp paired-end, dual-indexed sequencing were performed on the Illumina NextSeq 500 system.