GSM5745835: smallRNA_1703_D7M_1; Brassica napus; ncRNA-Seq
RNA was extracted from infected and uninfected plant tissue. For infected stems, cross-section disks (10 mm wide) were taken from the site of infection. The tissue was ground in an RNase-free mortar and RNA extracted using the Qiagen RNeasy Plant Mini kit (Toronto, ON, Canada). RNA was treated with Qiagen RNAse free DNAse (Toronto, ON, Canada), then cDNA synthesized using Quantabio qScript cDNA (Beverly, MA, USA). Quantity and purity were assessed spectrophotometrically and the quality of RNA samples verified using electropherogram profiles and RNA Integrity Numbers (RIN) (All samples had a RIN greater than 8.5) with an Agilent 2100 Bioanalyzer and RNA Pico Chips (Agilent, Santa Clara, CA, USA). cDNA libraries were constructed by Genome Québec according to their mRNA stranded library protocol. The Illumina HISeq4000 was used to generate 100 bp PE reads.