GSM5729284: Human skin equivalent containing fibroblasts - Replicate 2; Homo sapiens; RNA-Seq
The tissue was placed in dispase at 37C for two hours or until the epidermis could be peeled apart from the dermis. The epidermis was then trypsinized for 30 min. The dermis was digested in collagenase for 1 hour followed by trypsin for 30 minutes. Epidermal and dermal cells were independently incubated with DNAse for 5 min, filtered over a 40um cell strainer, recombined into the same solution, and flow sorted for live cells for library preparation. Single cells were captured using the Chromium Platform (10X Genomics) and libraries were generated using Single Cell 3' v3 chemistry. Libraries were sequenced on an Illumina Next-Seq6000 platform (Illumina). Cell counting, capture, library preparation, quality control, and sequencing was performed at the Genomics High Throughput Sequencing facility at the University of California, Irvine. Single Cell 3' v3 chemistry - 10X Genomics