HDM sample from vendor AirMid
500 mg milled raw materials of HDM from Greer and AirMid were processed and analyzed for metagenomic analysis by CosmosID. In brief, DNA was isolated from dust mite powder samples using the QIAGEN DNeasy PowerLyzer PowerSoil Kit, according to the manufacturers protocol. Isolated DNA was quantified by Qubit. DNA libraries were then prepared using the Illumina Nextera XT library preparation kit. Library quantity was assessed with Qubit (ThermoFisher). Libraries were then sequenced on an Illumina HiSeq platform 2x150bp. Unassembled sequencing reads were directly analyzed by CosmosID bioinformatics platform (CosmosID Inc., Rockville, MD) for multi-kingdom microbiome analysis and profiling of antibiotic resistance and virulence genes and quantification of organisms' relative abundance as previously described (Ponnusamy et al., 2016).