AMPLICON of influenza A virus : genome
wildtype and mutant recombinant A/WSN/1933(H1N1) viruses were rescued and passaged to P3 at 37oC, 5% CO2, and MOI 0.001, the viral RNA was extracted, and genome segment amplicons were amplifed by consensus primers MBTuni-12 and MBTuni-13, the PCR prodcuts were constructed in to library