GSM4981319: Mouse 2_tail; Mus musculus; RNA-Seq
Total RNA from frozen tissues was extracted after homogenization using TriPure Isolation reagent (Roche) and treated with TURBO DNA-free™ Kit (Thermo Fisher Scientific). cDNA libraries were prepared on ribo-depleted total RNA using Illumina Stranded Total RNA (Illumina, RS-122-2201, San Diego, CA) protocol with TruSeq V4 chemistry and sequenced in the Sequencing Facility of NCI on Illumina HiSeq 2500 with 2×125 nts modality and depth of 100 million reads per sample.