The Long chain Diol Index re-evaluated: metabarcoding study
DNA was extracted using a DNEasy Powerlyzer PowerSoil Kit (Qiagen), PCR primers were NGS-p23SrV_f1 (5-TCG TCG GCA GCG TCA GAT GTG TAT AAG AGA CAG GGA CAG AAA GAC CCT ATG AA-3) and NGS-p23SrV_r1 (5- GTC TCG TGG GCT CGG AGA TGT GTA TAA GAG ACA GTC AGC CTG TTA TCC CTA GAG-3) (Presting 2006; Sherwood and Presting 2007)