Illumina HiSeq 2500 sequencing; RNA-seq of cell lines containing p53-R273H or p53-R273H 22/23
RNA-seq of cell lines containing p53-R273H or p53-R273H 22/23
RNA was collected using Trizol H1299 cells were transfected with Lipofectamine 2000 with either pIRES puro (empty vector), pIRES puro containing p53-R273H, p53-R273H 22/23, or p53-R273H 22/23 53/54 cDNA.  The p53 mutations were created by cloning p53-WT cDNA into the pIRES puro vector and performing site-directed mutagenesis. RNA was extracted using Trizol reagent Libraries were constructed using Illumina TruSeq Stranded Total Ribo-Zero