NextSeq 550 paired end sequencing; Kidney RNAseq data from adult F344 and Hsd11b2 knockout rats (Model of SAME)
Kidney RNAseq data from adult F344 and Hsd11b2 knockout rats (Model of SAME)
All rats were male; 6 F344 controls and 6 Hsd11b2 knockouts (by ZFN targeting). All rats ~ 24-25 weeks old; kidneys excised, weighed and immediately snap frozen on dry ice prior to RNA prep Triazol extraction followed by DNAse treatment and RNEasy column; RNA qc tests: Qubit RNA BR kit; Qubit DNA HS kit; Agiulant Bioanalyser RNA6000 Nano kit (RIN numbers between 7.3 and 8.2 Tru-Seq Stranded total RNA QC with Kapa Universal Illumina Library quantification kit