GSM5835144: ovary tissue_PCOS_2; Rattus norvegicus; RNA-Seq
Ovaries were removed, flash frozen on liquid nitrogen then stored at -80°, and RNA was harvested using Trizol reagent.  NEBNext® UltraTM   RNA Library Prep Kit (NEB, USA)  was used with 3 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols