GSM5452247: ChIPseq-Ezh2-D0; Homo sapiens; ChIP-Seq
A549 cells were mixed with 1.4x10^5 mouse JM8 embryonic stem cells as spike-in control as described in Fursova et al 2019. ChIP assay were mainly performed as described previously (Brookes et al, 2012) with minor modifications described in Asenjo et al 2020. cChIP-seq libraries were generated from 3 ng of immunoprecipitated DNA with the NEBNext Ultra DNA Library Prep kit for Illumina (#7370) according to manufacturer's instructions.