GSM5381886: control 1135; Homo sapiens; RNA-Seq
Total RNA was isolated from HEK293T cells transiently or stably transfected with pb-NHIP-eGFP or negative control pb-NEG-eGFP using AllPrep DNA/RNA/Protein mini kit (Qiagen, Hilden, Germany). Human tissue total RNA samples were obtained commercially, including placenta (Life Technology, Carlsbad, CA, USA), testes (TaKaRa Bio, Kusatsu, Shiga, Japan), and fetal brain (Cell Applications, San Diego, CA, USA). RNA was extracted from frozen placenta samples in the Discovery group samples using TRIzol Reagent (Invitrogen, Carlsbad, CA, USA). RNA from cells and brain was prepared for RNA-seq library using Kapa RNA HyperPrep kits (Roche, Basel, Switzerland) together with the QIAseq FastSelect Human ribodepletion kit (Qiagen, Hilden, Germany). Libraries were assessed for quality and quantify on Agilent Bioanalyzer 2100 and pooled for multiplex sequencing with at least 25 million reads with 150 bp paired-end on Illumina NovaSeq 6000 (San Diego, CA, USA) by DNA Tech Core at University of California, Davis (Davis, CA, USA).