GSM5335058: t49; Trachemys scripta; miRNA-Seq
Plasma was thawed on ice and centrifuges at 3000 x g for 5 min at 4°C. An aliquot of 100 mL per sample was transferred in a new tube and small RNA was extracted using miRNeasy Serum/Plasma kit (Qiagen, Cat. No 217184), in accordance with the manufacturer's instruction. Small RNA transcripts were converted into barcoded cDNA libraries. Library preparation was performed using the NEBNext Multiplex small RNA Library Prep Set (Cat No NEB#E7560)