GSM5067747: 2D-monolayer INS-1 cells R1; Rattus norvegicus; RNA-Seq
After 5 days in culture total RNA was isolated from 2D monolayer culture and 3D hydrogel culture group using an RNeasy Mini kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions (biological replicates, passage 3).  After isolation, the total RNA was treated with the Turbo DNAfree kit according to the manufacturer's instructions (Thermo Fisher Scientific). The RNA concentration and quality were evaluated by Nanodrop and Agilent2000 Bioanalyzer. RNAseq libraries were prepared using the TruSeq stranded mRNA kit. The RNAseq libraries were sequenced using an Illumina NextSeq 500 platform, resulting in 75bp single end reads