RNA-seq of E. coli: empty vector
Aerobic growth in LB medium at 37 degrees celsius, 180 rpm until OD600nm = 0.6. Addition of 1 mM IPTG and growth for an additional hour. 5.10^9 cells pelleted and flash-frozen in liquid nitrogen. Total RNA purification (MAXWELL, PROMEGA), rRNA depletion (Ribo-Zero rRNA removal kit, Illumina), cDNA first-strand synthesis and cDNA libraries preparation (TruSeq Stranded mRNA Library Prep kit, Illumina)