WGBS of pig fetal LD skeletal muscle
DNA was isolated using the PureLink Genomic DNA Kit (Invitrogen), and quality and quantity were assessed on the Qubit fluorometer. DNA samples were spiked with unmethylated lambda phage DNA (5 ng lambda DNA/ 1ug sample DNA) to assess bisulfite conversion rates. Sodium bisulfite conversion was performed using the Zymo EZ DNA Methylation-Gold Kit, and sequencing libraries were prepared using the Kapa Hyber Prep DNA Kit (Roche). WGBS was performed on an Illumina HiSeq 4000 instrument in 2x150 PE format at an average depth of 18X per sample.