GSM5288624: N1-2; Homo sapiens; RNA-Seq
Total RNA of plasma exosome  was isolated using Magen Hipure Total RNA Mini Kit(Magen,Guangzhou,China) according to the protocol RNA-seq library was prepared with approximately 100ng of total RNA using KAPA RNA HyperPrep Kit with RiboErase (HMR) for Illumina® (Kapa Biosystems, Inc., Woburn, MA).The RNA was purified with VAHTS RNA Clean Beads. The RNAs was fragmented and then first strand and directional second strand synthesis were performed. Then the A tailing and adapter ligation