Illumina 150 bp reads (paired-end) of Saccharomyces cerevisiae JP1 genomic DNA
Genomic DNA was extracted from a pure culture using Wizard kit (Promega), DNA concentration measured with QUBIT (74.8 ng/uL), library prepared following Nextera DNA Flex, QUBIT after 23.5 ng/uL, average fragment size (Bioanalyzer) 677 bp, sequencing on a Illumina NextSeq 550 equipment, cartridge High Output for 300 bp reads (2x 151 bp), capable of generating 400 million single reads