GSM5692892: B6-CD8neg cDC2 #1; Mus musculus; RNA-Seq
Total RNA was extracted using an RNeasy Mini Kit (QIAGEN) and residual genomic DNA removed using Rnase-free Dnase (QIAGEN). Sample libraries were constructed using Illumina TruSeq Stranded mRNA kit. Libraries were quantitated using Qubit DNA HS kit (Thermofisher Scientific) and checked for adaptor contamination using Bioanalyzer 2100 (Agilent Technologies). Sample libraries were then sequenced by Micromon Genomics at Monash University on NextSeq500 using High-Output SBS chemistry (Illumina) at 1.8pM library concentration and 1x75b read length.