16SFt+CA:Rep1
2-step PCR with an enzymatic cleanup in between. First: 16S Universal primers 341F and 806R. Second: concatenated primers including Illumina P5 or P7 adapters. Triplicate reactions were pooled and cleaned with magnetic beads. Final pool was purified and measured in Qubit. Libraries were denatured and loaded in the Miseq lane and sequenced in 500 cycles.