WGS of Erwinia amylovora
DNA was extracted according to the Nucleospin Plant II protocol (Macherey-Nagel GmbH & Co. KG, Germany) starting from 250 l aqueous solution of pure culture. The DNA was eluted with 70 l H2O. The extracted DNA has been used to construct the next generation sequencing libraries, starting from 100ng each and using the Kapa Hyperplus library kit (Roche) combined with the Kapa Unique Dual Index adapters (Roche) following the manufacturers instruction. The pooled library was quantified by qPCR and the fragment insertion size of 500bp was evaluated on the Agilent 2200 TapeStation. The final library was sequenced at IGA Technology on NovaSeq 6000 in paired-end 150 mode.