Miseq for base editing mouse
amplicons for Apc.1405,Smad4.224. [Small intestine 2D cells lenti gRNA 1405 12hr dox replicate 3] [si HOM organoids targeted with 1405/135/224/545 synthetic gRNA EN (remove RspoI) + nutlin + Tgfbeta replicate 3 224 PCR]