Illumina HiSeq 2500 paired end sequencing; RNA-Seq of maize mesocotyls exposed to osmotic stress
RNA-Seq of maize mesocotyls exposed to osmotic stress
Maize mesocotyls were collected from 6-d etiolated seedlings after 10% PEG 6000 treatment of roots for 60 min for RNA extraction.  Maize seeds were germinated at 28oC and grown in Hoglandâ€™s nutrient solution in the dark for 6 days and subjected to PEG treatment. CTAB PBIOZOL and ethanol precipitation protocol was used for extraction and purification of total RNA from maize mesocotyl tissue. mRNA was fragmented and enriched with by oligo-dT beads magnetic beads. Sequencing library was amplified with the broken mRNA as the templates to synthesize cDNA. The cDNA templates were purified with the kit and enriched by PCR.