GSM5452261: PAM-1 SE 4C-seq; Mus musculus; OTHER
Paraformaldehyde fixed cells were digested with TatI restriction enzyme digestion, incubated overnight at 37ºC and circularized using T4 DNA ligase. Gradient range of annealing temperature (55-65ºC) were used to determine the optimum annealing temperature for inverse PCR. NEBNext Ultra II DNA Library Preparation kit for Illumina (NEB)