GSM5906722: KNPC_siCtrl_rep2; Mus musculus; RNA-Seq
Total RNA was harvested by TRIzol method from triplicate samples and quality was assessed by running 500 ng RNA on an agarose gel. Libraries were prepared from 500 ng RNA using Truseq RNA Library Prep Kit v2 according to manufacturer's instructions. mRNA was captured by RNA purification beads and eluted. The first strand and second strand cDNA were synthesized and the overhangs of the fragments were repaired into blunt ends. The 3' end was added with a single adenine to prevent mutual ligation during the following adapter conjugation. Finally, the DNA fragments with both flow-cell-hybridization adapters were amplified and the library was quantified by Agilent Bioanalyzer 2100.