GSM5693365: H2030-BrM pooled GFP+ metastases from three nude mice; Homo sapiens; RNA-Seq
After obtaining the GFP+ cells the sample was loaded onto a 10x Chromium Single Cell controller chip B (10x Genomics) as described in the manufacturer's protocol (Chromium Single Cell 3'GEM, Library & Gel Bead Kit v3, PN-1000075). Intended targeted cell recovery of ~10000 cells. Generation of gel beads in emulsion (GEMs), barcoding, GEM-RT clean-up, cDNA amplification and library construction were all performed as recommended by the manufacturer. Cell sample was loaded onto a 10x Chromium Single Cell controller chip B (10x Genomics) as described in the manufacturer's protocol (Chromium Single Cell 3'GEM, Library & Gel Bead Kit v3, PN-1000075). Intended targeted cell recovery of ~10000 cells. Generation of gel beads in emulsion (GEMs), barcoding, GEM-RT clean-up, cDNA amplification and library construction were all performed as recommended by the manufacturer. Single-cell RNA-seq libraries were sequenced with an Illumina NextSeq 550 (using v2.5 reagent kits) in paired-end fashion (28bp + 56bp bases).