whole exome sequencing of mouse lung derived cell lines
fragmentation was carried out using the hydrodynamic shearing system (Covaris) to generate 180-280bp fragments and remaining overhangs were converted into blunt ends. After adenylation of the 3 ends, adapter oligonucleotides were ligated and selectively enriched in a PCR reaction. The libraries were then hybridized with a biotin labeled probe, and magnetic beads with streptomycin were used to capture exons. Captured libraries were enriched in a PCR reaction to add index tags to prepare for hybridization.