GSM5729286: Xenografted human skin equivalent - Mouse Alignment; Homo sapiens; RNA-Seq
The xenografted tissues were dissected from the NSG mice that they were grafted on to. The tissues were then placed in dispase overnight, at 37C, until the epidermis could be peeled apart from the dermis. The epidermis was then trypsinized for 30 min, and the dermis was discarded. Epidermal cells were incubated with DNAse for 5 min, filtered over 40um cell strainer, and flow sorted for live cells for library preparation. Single cells were captured using the Chromium Platform (10X Genomics) and libraries were generated using Single Cell 3' v3 chemistry. Libraries were sequenced on an Illumina Next-Seq6000 platform (Illumina). Cell counting, capture, library preparation, quality control, and sequencing was performed at the Genomics High Throughput Sequencing facility at the University of California, Irvine. Single Cell 3' v3 chemistry - 10X Genomics