GSM5835293: X186_HF_WT; Mus musculus; RNA-Seq
Whole lung was removed from mouse and flash-frozen in liquid nitrogen and stored in -80C until shipment for further processing. Total RNA-Seq library construction was performed from the RNA samples using the TruSeq Stranded RNA Sample Preparation Kit and bar-coded with individual tags following the manufacturer's instructions (Illumina, Inc. San Diego, CA). An Agilent Bravo Automated Liquid Handling System was used to prepare libraries. Total RNA-Seq mRNA, 35 million reads per sample, 2 lanes per sample, paired-end run.