GSM5833751: E7_DMSO_d6; Homo sapiens; ATAC-seq
Omni-ATAC was performed as before (Liu et al. 2021) with 70,000 HUDEP-2 cells frozen in 10% DMSO/FBS. On the day of the experiment, cells were thawed on ice and permeabilized with 50 μL RSB buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2) with 0.1% NP40, 0.1% Tween-20 and 0.01% Digitonin for 3 minutes on ice. 1 mL of cold RSB containing 0.1% Tween-20 was added before cells were pelleted again. The pellets were then resuspended with 50 μL transposition mixture (25 μL 2xTD buffer, 2.5 μL transposase from ATAC-seq kit (Illumina), 16.5 μ