Native  soil metagenome from Tridax
The DNA was subjected to end-repair/dA tailing using 3 microlitres of UltraII End prep enzyme mix in a 60 microlitre reaction volume in a 0.5 ml PCR tube for 5 minutes at 20 degree celcius and for 5 minutes at 65 degree celcius. The modified DNA fragments were purified using AMPure XP beads. The DNA was eluted in a 25 microlitre volume of MQ water . Recovered DNA was quantified using Qubit reader (ThermoFischer Scientific)