GSM6138747: Day 14 IRE1α WT 19; Mus musculus; RNA-Seq
Single cell suspensions were generated and stained with CD45, and Epcam, without fixation. Viable CD45- mCherry+ Epcam+ cells were sorted into Trizol. mRNA was extracted as previously described. mRNA concentration was then evaluated by Nanodrop (Thermo Fisher), and the quality of the mRNA was evaluated by Bioanalyzer (Agilent). cDNA libraries were generated using the Illumina TruSeq RNA Sample Preparation kit V2 with non-stranded Poly A selection, in accordance with the manufacturers protocol. 2x50 bp single-end sequencing was performed on the HiSeq 4000 sequencer.