GSM4981828: WT, vehicle control, replicate 1; Mus musculus; RNA-Seq
MBMECs were harvested directly from transwell inserts in 350μl RLTplus lysis buffer (3 inserts/condition/350μl RLTplus). RNA was isolated using an RNeasy Plus Microkit (Qiagen, Hilden, Germany) according to the manufacturer's protocol. Genomic DNA contaminants were digested on microkit columns during RNA isolation. RNA concentrations were measured via QuBit 4 fluorometer (ThermoFisher Scientific, Schwerte, Germany). For RNA sequencing, RNA was frozen immediately after isolation and stored at -80°C until its transfer to the sequencing facility. mRNA library preparation with equal amounts of RNA from each sample (100ng) was achieved with VAHTS Stranded mRNA-seq Library preparation kit (Vazyme) following manufacture's protocol.