Near whole genome sequence of Ndumu virus
Viral RNA was extracted, DNase treated and sequence-independent single primer amplification (SISPA) performed using random primers 9FR-26-RV-N).  The second strand was synthesised, cDNA purified and amplified using primer FR-20-RV.  Multiplexed paired-end libraries were prepared using the Nextera DNA Flex sample preparation kit, dsDNA was fragmented and tagged with adapters and PCR amplification was used to affix unique dual index sequences to each fragment end.  DNA was quantified using a Qubit fluorometer and equimolar library pools loaded on the NextSeq 550.