GSM5287760: ChIPseq_H3K4me3_Day8plus_rep2; Mus musculus; ChIP-Seq
Lysates were prepared from cells fixed with 0.5% formaldehyde and subjected to sonication to release protein-DNA complexes with 200-300 bp in length. Histone-DNA complexes were obtained by immunoprecipitation with specific histone methylation antibodies. After proteinase K treatment and reverse-crosslinking, DNA fragments were purified using QIAquick PCR Purification Kit (QIAGEN). Library preparation was carried out using ThruPLEX DNA-Seq Kit (TAKARA BIO) according to the manufacturer's protocol.