GSM4982819: pig_CC_DNAme_rep2; Sus scrofa; Bisulfite-Seq
STEM-Seq library preparation  The whole genome bisulfite libraries for bovine and porcine FGO were constructed with STEM-seq as previously described (Zhang et al., 2018a). Briefly, the oocytes were firstly lysed with 10μL lysis buffer and 1μL protease K at 55°C for 3 hours. The protease K was inactivated at 72°C for 40 minutes. After lysis, the spike-in λ-DNA (Promega; D150A) was added with a mass ratio of 1:200. Then the mixture was treated and then purified with the bisulfite conversion reagent with the EpiTect Fast Bisulfite Conversion Kit (Qiagen; 59824) following the manufacture's instruction, with a modified protocol: denature for 8 min at 95 °C, incubate at 60 °C for 25 min, and repeat the procedure once. Finally, the purified converted DNA was subject to TELP library construction process as previously reported (Peng et al., 2015).