Transcriptome sequence reads of Pythium myriotylum
The RNA quality assessment was done using RNA ScreenTape System (Catalog: 5067- 5576, Agilent) in a 4150 TapeStation System (Catalog: G2992AA, Agilent).The enriched mRNAs were primed with NEBNext Random Primers and chemically fragmented in a magnesium-based buffer at 94C for 10 minutes. The fragmented mRNAs were reverse transcribed to form cDNA and the first strand cDNA reactions were converted to dS DNA. The double stranded cDNA fragments obtained were cleaned up by using 1.8X of AMPure XP beads (Catalog: A63881, Beckman Coulter). The samples were further purified using 0.9X AMPure XP beads (Catalog: A63881, Beckman Coulter) and the DNA was then amplified by 12 cycles of PCR using NEBNext Ultra II Q5 master mix, and to facilitate multiplexing, NEBNext Multiplex Oligos for Illumina kit was used . The amplified products were purified using 0.9X AMPure XP beads (Catalog: A63881, Beckman Coulter) and the final DNA library was eluted in 15 uLs of 0.1X TE buffer.The QC passed libraries were sequenced on Illumina HiseqX to generate 2X150 bp paired end reads.