resequencing of Arabidopsis slis3
To clone SLIS2 and SLIS3, we decided to use MutMap strategy. The lis slis2 and lis slis3 mutants were backcrossed with lis. In the F2 population, more than 51 plants with suppressor phenotype were sampled as the suppressor pool. The DNA from both the suppressor pool and the lis pool was sequenced using the next-generation sequencing technology.