GSM6045551: PNI day14-CD11c-Positive 5; Mus musculus; RNA-Seq
Microglia cells were FACS-sorted into 1.5-ml tube containing lysis buffer (TaKaRa 635013) with RNase inhibitor (TaKaRa 2313A). After samples were mixed briefly by vortexing and spinning down, the lysates were used for library preparation Libraries were prepared using CEL-Seq2 protocol and primers (Hashimshony T et al.) except that MaximaH minus reverse transcriptase (Thermo Scientific, #EP0751) was used for single-stranded synthesis and Second Strand Synthesis Module (NEB, #E6111) for double-stranded cDNA synthesis