GSM6198712: CONTROL_scRNAseq; Mus musculus; RNA-Seq
Pancreata from one control mouse (sample 1) and two CP mice (sample 2) were enzymatically digested into a single-cell suspension, respectively. Freshly dissected tissue was laid placed on into a 10-cm tissue culture dish and cut into pieces using a sterile razor blade. After washing twice with PBS, each sample was moved to a 15-mL tube containing pre-warmed 37°C digestion buffer comprising 1% FBS, respectively. The tubes were incubated on a shaker at 37°C for 40 min at 37°C. Then, the cells were resuspended and washed three times in PBS before debris was filtered out with using a 30-μm mesh filter. RNA libraries were generated by BD Rhapsody single cell RNA-seq and prepared for sequencing using standard Illumina protocols.