GSM5335174: KL_rep2; Mus musculus; RNA-Seq
Lung tumor samples were freshly dissected and preserved in RNAlater, and RNA was extracted using RNeasy Mini Kit (QIAGEN) and treated with DNase. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. mRNA-seq libraries were prepared with the SureSelect Strand Specific RNA library prep kit (Agilent Technologies) after PolyA capture, and then sequenced by single-end 75 base pairs using the Illumina NextSeq 500 platform.