GSM5692757: d30_rep1; Homo sapiens; OTHER
We extracted genomic DNA from both the reference and final cell populations using the QIAamp DNA Blood Maxi kit (Qiagen) according to the manufacturer's instructions. We amplified sgRNAs integrations from 500 μg genomic DNA by PCR with indexed sgRNA sequencing library primers containing Illumina adaptors and sequenced them on a Miseq using custom Illumina sequencing and index primers.