GSM4980355: 1_HAEC_2.50; Homo sapiens; Bisulfite-Seq
Genomic DNA was isolated from HEACs treated with 125 mg/dL and 250 mg/dL D-glucose using DNeasy Blood and Tissue Kit (Qiagen) according to the manufacturer's instructions. DNA concentration and purity were determined using NanoDrop 2000 (Thermo Scientific). DNA integrity was verified using elecrophoresis on 1% agarose gel. Hybridized molecules were clonally amplified and annealed to sequencing primers with reagents from an Illumina HiSeq PE Cluster Kit v4-cBot (PE-401-4001).