GSM5744046: CD34(high)EPCR-positive_rep2; Homo sapiens; RNA-Seq
Cultured cells were staind by APC labeled anti-human CD34 (BioLegend, 343510), BV605 labeled anti-human CD201(EPCR) (BD Bioscience, 745335) for 30 minutes at 4℃. Dead cells were labeled by propidium Iodide (BioLegened, 421301). CD34(high)EPCR-positive and CD34(high)EPCR-negative cells were sorted by MoFlo (Beckman Coulter) and stored in TRIZOL-LS reagent (Invitrogen, 0296010) at -80℃. rRNA-depletion and library synthesis were performed by Takara SMARTer kit.