GSM5501724: Sal+AMPHMulti-seqBarcoded PV Nuclei; Mus musculus; RNA-Seq
Following treatment, Nucleus Accumbens tissue was dissected fresh before nuclei were isolated and FANS sorted for DAPI + GFP co positivity. 2,125 GFP+ nuclei were sorted into final volume of 40uL before loading 10X Chromium controller with 3' Gene Expression (v3) kit for single nucleus RNA-seq Libraries were constructed according to the 10X Genomics protocol for 3' Gene Expression (v3) snRNA-seq