GSM4981056: Peripheral neutrophils healthy donor1; Homo sapiens; RNA-Seq
Neutrophils were isolated from whole blood by negative selection using MACSxpress whole blood neutrophils isolation kit (Miltenyi Biotec). Purity of neutrophils was > 97.9 RNA was purified with the Direct-zol RNA Microprep or Miniprep Kit (Zymosan), according to manufacturer's instructions. RNA quality control was performed with the 4200 Tape Station system (Agilent). Total-RNA-sequencing library preparation was performed starting from 1 ng of total-RNA with the SMART-Seq Stranded Kit (Clontech-Takara). Libraries obtained were qualitatively assessed by using TapeStation 4200 (Agilent) and quantified by Qubit Fluorimeter (Thermofisher). Afterwards, they were multiplexed in equimolar pools and sequenced on a NextSeq-550 Illumina Platform generating at least 80 million 75bp-PE reads per sample