Functional metagenomic library of pooled metagenomic DNA from stool samples of Dutch travelers
Metagenomic DNA from 10 random post-travel samples were pooled together to construct a functional metagenomic library. The pooled metagenomic DNA was cloned into an expression vector and transformed into E. coli indicator hosts. These E. coli transformant libraries were then screened against antibiotics at selective concentrations, and the cloned insert DNA in surviving transformants was sequenced using Nextera protocol