RNA-Seq of liver from healthy tilapia at 6 day after initiating TiLV- challenge experiment, biological replicate 2
The cDNA sequencing library from total RNA has been prepared following the protocol and using the consumables given with NEBNext ultra directional library prep kit (New England BioLabs, MA, USA). The library was quantified using Qubit fluorometer and its fragment size distribution was analyzed on Agilent 2200 Tape system. Sequencing of library was performed on Illumina NextSeq500 with 2x150 bp paired-end chemistry to generate sequence reads.