GSM5288677: Intact LAP_03 [1314D7]; Homo sapiens; RNA-Seq
Monocytes isolated from different groups of sepsis patients stratified by LAP responses were lysed in Trizol reagent and stored at −80°C. Total RNA was extracted from cells using the QIAGEN RNeasy RNA extraction kit, using on-column DNaseI treatment. Ribosomal RNA was removed using the riboZero rRNA removal kit. All samples had good qualities (RIN score >=8). RNA libraries were prepared to enrich for polyA tail-RNA using Bioscientific NEXTflex-Rapid-Directional mRNA-seq kits. RNA libraries were prepared with the Caliper SciClone system.