SEA_0001_2010_06_21_Vibrio_MAG
Metagenomicsequencing(Hiseq):WeusePicogreen(Invitrogen)toquantitategenomicDNAsamples.DNAisshearedusingaCovarisandlibrarieswereconstructedwiththeNugenOvationUltralowLibraryprotocol.Weaimforaninsertsizeof175bptoenablereadmerging.AmplifiedlibrariesarevisualizedonanAgilentDNA1000chiporCaliperHiSensBioanalyzerassay,pooledatequimolarconcentrationsbasedontheseresults,andsizeselectedusingaSagePippinPrep2%cassette.ThelibrarypoolisquantifiedusingaKapaBiosystemsqPCRlibraryquantificationkit,thensequencedontheIlluminaHiSeq1000ina2x100paired-endsequencingrunusingdedicatedreadindexing.ThesamplesaredemultiplexedwithCASAVA1.8.2.