GSM5620333: MMV687807 rep 3; Vibrio cholerae; RNA-Seq
700 µl of cells were incubated with 100 µl of 8x lysis buffer (0.8% SDS and 16 mM EDTA) and an equal volume of acidic phenol and incubated at 65 oC for 5 minutes, followed by incubation on ice for 10 minutes.  After phase separation by centrifugation for 2 minutes at 13,000 x g, the aqueous phase was isolated and combined with an equal volume of anhydrous ethanol.  RNA was purified using the Direct-zolTM RNA miniprep kit (Zymo Research) with an in-column DNaseI digest to remove contaminating DNA.  After quantification of RNA, the ribosomal RNA was removed with the RiboZero kit from Illumina. RNA libraries were prepared for sequencing using standard Illumina protocols