GSM5692703: Sample_87877; Mus musculus; RNA-Seq
Glioma bearing mice prefused with sucrose, brains were removed, cryopreserved in OCT and  cryosectioned at 10 µm thickness. Tumor sections were fixed in ethanol and stained with 4% Cresyl violet and 0.5% eosin. Oncostream areas from 3 independent mouse glioma samples were dissected from surrounding glioma tissue using a LMD7000 Leica laser capture microdissection. RNA was isolated for laser microdissected tissues using the RNeasy Plus Micro Kit following the manufacturer recommendations (Qiagen). sequenced on the Illumina HiSeq 4000, as paired-end 50 nt reads with 10% PhiX spike-in, according to manufacturer's recommended protocols. RNA libraries were prepared for sequencing using standard Illumina protocols