GSM5833807: NT-6h_H3K9me3_rep2; Mus musculus; ChIP-Seq
All samples were washed three times in 0.5% bovine serum albumin in phosphate-buffered saline (BSA-PBS). Chromatin was fragmented by MNase and was incubated with antibody bound beads at 4℃ for 2 hours. Captured DNA was wahed and isolated by isopropanol. Libraried were generated using the KAPA HyperPrep Kit for the Illumina platform (kk8504), following the manufacturer's instrunctions. Paired-end 150 bp sequencing was performed in NovaSeq (Illumina) platform in Berry Genomics and Novogen.