RNA-Seq of mus musculus:  agd male  mice-derived 2cell
two cell embryos  were transferred into the lysis buffer for reverse transcription. A poly(A) tail was added to the 3 end of the first-strand cDNA using terminal deoxynucleotidyl transferase. The total cDNA library was used for RNA-seq library construction after 1820 cycles of amplification. Paired-end 150-bp sequencing was performed