GSM5288669: Sample-4: Whole bladder cells female NMIBC Hgf-Cdk4R24C; Mus musculus; RNA-Seq
Bladders were dissociated with the mouse tumor dissociation kit (Miltenyi cat. 130-096-730) according to the manufacturer's instructions to create single cell suspensions. Samples were submitted to downstream procedures if viability was 75% or higher. Samples were processed using Chromium Single Cell 3' GEM, Library & Gel Bead Kit v3 (10x Genomics cat. 1000092) according to the manufacturer's instructions. Samples were processed using Chromium Single Cell 3' GEM, Library & Gel Bead Kit v3 (10x Genomics cat. 1000092) according to the manufacturer's instructions.