ITS of fungi: Fungal diversity in sediments of an Oxygen Minimum Zone
The environmental DNA direct extraction was performed for each marine sediment subsample employing the DNeasy PowerSoil kit (Qiagen, Carlsbad, CA, United States). The amplification and Illumina Mi-seq paired-end sequencing (2300) were targeting the internal transcribed spacer ITS1 of the ribosomal RNA gene cluster with the primers set ITS1F and ITS2, obtaining around 25,000 reads per subsample.