GSM5452229: 714-HB: WT_hindbrain rep3; Mus musculus; RNA-Seq
Total RNA was extracted from dissected fore brain, mid brain and hind brain of 3 biological replicates P-25 wild type and Fmr1CRE knockin mutant mice from same litter using the RNeasy kit (QIAGEN) combined with QIAshredder (QIAGEN), following the manufacturer's instructions. Random primed cDNA from poly(A) selected RNA was converted into an Illumina sequencing library using RNA Library Prep Kit from Illumina (E7420, NEB, USA) in conjunction with NEBNext® Multiplex Oligos for Illumina (E7335/E7500, NEB, USA). Single-end 50-base pair (bp) reads were generated using a NextSeq 500 (Illumina Inc, SY-415-1002)