GSM5384465: Conditioned successful 3 RNA-seq; Mus musculus; RNA-Seq
Total RNA and protein were isolated from mouse brain septum using the NucleoSpin miRNA kit (Macherey-Nagel GmbH & Co KG) according to the manufacturer's protocol for animal tissues. Libraries for total RNA-seq were prepared using the Ovation SoLo RNA-Seq System, Mouse (#0501-32, NuGEN Technologies) according to the manufacturer's protocol with an RNA starting concentration of 0.8 ng/µl.