GSM6217570: D1-CD137; Mus musculus; RNA-Seq
At the indicated time points, CD8+ T cells were isolated from the BM using mouse CD8+ T cell isolation kit (Miltenyi Biotec) and FACSaria™ Fusion (BD biosciences). Single-cell libraries (3′ gene expression) were generated using the Chromium Controller Instrument and Chromium Single Cell 3′ Library & Gel Bead Kit v3 according to the manufacturer's protocol (10× Genomics).  Single-cell library size and quality were confirmed on the Fragment Analyzer (Agilent). KAPA Quantification Kit for Illumina platforms (KAPA Biosystems, Roche) was used to quantify libraries. The libraries were sequenced on a NextSeq 550 (Illumina) in pair-end sequencing 28 bp (read1) × 91 bp (read2) and a single index 8 bp in length.