16S rRNA sequencing from the gills of Argopecten purpuratum form the field
The 16S rRNA gene of bacterial communities was amplified and sequenced, targeting the variable regions V3-V4 (341F: 5-CCTACGGGNGGCWGCAG-3; 805R: 5-159 GACTACHVGGGTATCTAATCC-3) and using gDNA from 6 equimolar pool of gDNA from 10 scallops from each experimental condition