GSM5573729: G2_DMSO treated_Vehicle control; Homo sapiens; RNA-Seq
Total RNA was isolated using an RNA extraction reagent prepared in-house. Libraries were prepared using TruSeq RNA Kit. according to the manufacturer's instructions. Briefly, mRNA was fragmented and reverse transcribed using oligo-dT primers to synthesize cDNA. The double-stranded cDNAs were end-repaired, polyadenylated, and ligated with adapter sequences followed by size selection for approximately 250-450 bp using AMPure XP beads. Uracil-containing strands were degraded by treatment with USER Enzyme. Sequencing was performed using Illumina Novaseq 6000 Platform in paired-end reads format with the Phred score of 64.