Miseq for base editing mouse
amplicons for Apc.1405, Smad4.224.  [Small intestine 2D cells lenti gRNA 1405 Day 7 dox switch replicate 1] [si HOM organoids targeted with 1405/135/224/545 synthetic gRNA EN (remove RspoI) + nutlin + Tgfbeta + Selumetinib replicate 2 224 PCR]