GSM4851994: Human Liver Biopsy  PT-19; Homo sapiens; RNA-Seq
Collected  liver tissue samples were placed in complete media (RPMI+10% FBS) on ice and  immediately further processed. Single cell suspensions were generated by  mincing freshly obtained liver tissue specimen into 1 mm cubic pieces,  followed by enzymatic digestion using the Miltenyi tumor dissociation  kit. Digestion was carried for 20 minutes at 37°C with constant shaking and stopped by adding RPMI +20%FBS. The digested tissues were then passed through a 70 um cell strainer and centrifuged at 350 g for 10 minutes. RBC lysis was carried out with ACK lysis buffer (Thermo Fischer Scientific Cat no. A1049201) and cells were washed with complete media and once more strained through a 45 µm mesh. Library generation was performed according to manufactor´s protocol (Chromium Next EM Single Cell 3`GEM, 10000128)