GSM4981603: Aorta, Bcl11b WT_1; Mus musculus; RNA-Seq
Aortas were homogenized in Trizol and kept at -80C until RNA extraction with a commercially available column-based RNA purification kit (ZymoResearch, Irvine, CA, USA), following manufacturer's instructions. RNA quality (18S/28S ribosomal RNA ratio) was analyzed with a Bioanalyzer (Boston University Microarray Core) and samples with a RIN  7 were deemed of good quality and further processed. RNA was treated with the NEBNext rRNA depletion kit (E6310X, New England Biolabs) to deplete ribosomal RNA, and converted into double-stranded cDNA using NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module (E6111L, New England Biolabs). cDNA was subsequently tagmented and amplified for 12 cycles by using Nextera XT DNA Library Preparation Kit (FC-131, Illumina). Sequencing libraries were analyzed with Qubit and Agilent Bioanalyzer, pooled at a final concentration of 2.2nM, and sequenced on a NextSeq 500 (Illumina).