GSM5335955: Lane1_RPI6_85s; Zea mays; RNA-Seq
Maize anthers were dissected and pollen precursors were released by cutting the anther in half and gently pressing on the anther. For meiotic cells, the anther was fixed for 2 hours in ice cold 3:1 ethanol:acedic acid prior to cell release. Pollen precursors were then picked up manually using blunted syringe needles, and placed on the cap of an 8-tube PCR strip. Library protocol based on CelSeq2. Read 1 contained a 10 bp unique molecular identifier (Uninucleate Microspore (UM)I) followed by a 6 bp sample barcode. Read 2 contained transcript sequence. Deposited files are read 2 with the Uninucleate Microspore (UM)I appended to the fastq entry headers, all samples have been demultiplexed using the 6 bp sample barcodes.