GSM5381432: Embryo WT Rep 3; Drosophila melanogaster; RNA-Seq
The embryos were dechorionated in bleach, washed in water and 100% ethanol prior to RNA extraction using the ISOLATE II RNA Mini Kit's protocol (Bioline, UK). The extracted RNA was quantified using Nanodrop (Thermo Fisher Scientific). Library preparation was performed using 1µg of total RNA and sequencing was performed using Illumina HiSeq 4000 System (2 × 151 bp read length, 40 million reads per sample).