GSM5907319: m12_CD rep1; Mus musculus; RNA-Seq
Total RNA was extracted using Trizol and treated with DNAse1. Ribosomal RNA was removed from the samples using RIBO-ZeroTM Magnetic kit (Epicentre, Madison, WI). RNA was reverse-transcribed to cDNA and amplified using TruSeq™ RNA Sample Prep Kit v2 from Illumina, Inc (San Diego, CA). The cDNA library was similarly sequenced in Illumina HiSeq2500 sequencer. RNA libraries were prepared for sequencing using standard Illumina protocols