GSM5066798: MEF; Mus musculus; RNA-Seq
The beating colonies were picked at designated day and total RNA was isolated using Trizol reagent (Thermo Scientific). RNA quality was assessed by Agilent 2100 bioanalyzer using the RNA 6000 Nano Chip for Quan-seq 3' mRNA sequencing (Agilent Technologies). For the construction of library, Quant-Seq 3' mRNA-Seq Library Prep Kit (Lexogen, Inc., Austria) was used for gene expression analysis. Quant-seq 3' mRNA sequencing