GSM5216997: Pyraclostrobin 3.10-8M; Mus musculus; RNA-Seq
Cells were lysed 24h after chemical exposure and RNA extracted using a Macherey-Nagel NucleoSpin RNA II kit. RNA concentrations were measured with a Nanodrop spectrophotometer (Thermo Fisher Scientific). cDNA libraries were prepared using the total RNA SENSE kit (Lexogen, Vienna Austria) and deep sequencing was performed on a NextSeq500 (Illumina) sequencer