GSM5693338: RNAseq FoxA1 0.05 induced rep2; Homo sapiens; RNA-Seq
We extracted RNA from 1e6 cells/sample using the PureLink RNA Mini column extraction kit (Invitrogen #12183020) and completed on-column DNA digestion with PureLink DNase (Invitrogen #12185010). We generated libraries by following the NEBNext Ultra II Directional RNA Library Prep Kit (NEB #E7765S) and then size selected the libraries with AmpureXP beads.