RNA-seq of sphere-formed small cell lung cancer cell line(NCI-H446)
Sphere-formed group:Human SCLC cell line NCI-H446 was plated in a 6-well in ultra-low attachment microplate at a density of 5 x 103 cells/well. These cells were cultured in DMEM/F12(Gibco, USA) medium with EGF (20 ng/mL, Peprotech, USA), bFGF (FGF-basic, 20 ng/mL,Invitrogen), B27 (20 l/mL, Gibco) for two weeks.