Cycas calcicola RADseq popualtion level sample
Libraries were prepared using an Illumina TruSeq nano high-throughput dual index library preparation kit (Illumina Inc., CA, USA). We followed the ezRAD v3 modified protocol (Toonen et al. 2013) using half of the recommended volumes of the kit to save costs (Clugston et al. 2019). Following Clugston et al. (2019), the final steps of library preparation were modified from the ezRAD protocol by modifying the final bead clean with a 0.8:1 ratio of AMPure XP beads to remove adapter dimer. The libraries were validated using a LabChip, cleaned using a ratio of 0.9:1 AMPure XP beads, and quantified using a Qubit high sensitivity kit. Libraries were then normalised to a concentration of 10 nM, after which 5 L of each library was pooled for sequencing.