GSM5452723: OX_3; Arabidopsis thaliana; RNA-Seq
mRNA-seq experiments were performed in three independent biological replicates of the Col and ERF34-OX#1 plants. RNA was isolated using WelPrepTM (WelGENE, Korea). Total RNA integrity was checked using an Agilent Technologies 2100 Bioanalyzer with an RNA Integrity Number (RIN) value greater than 7.8. Poly(A) mRNA was isolated from the RNA (2 µg) and fragmented using Illumina Truseq™ Stranded mRNA LT Sample Prep Kit with poly-T oligo-attached magnetic beads. After the cDNA synthesis from the RNA fragments by using Superscript II reverse transcriptase (Life Technologies), the strand-specific cDNA libraries were generated with adaptor-ligation and sequenced by Illumina HiSeq 2500 system, according to the Illumina Directional RNA-Seq protocol.