GSM5453265: Tumor1 (Mouse 42369), scRNAseq; Mus musculus; RNA-Seq
Mouse tumors were cut with scalpels in small fragments. Enzymatic dissociation was performed in GIBCO medium containing 150 µg/mL Liberase™ TL Research Grade (5401020001, Merk) and 150 µg/mL DNase (DN25, Sigma Aldrich), for 30 minutes at 37°C with 400 rpm agitation. Cell suspension was then filtered using a 70 µm cell strainer (130-098-462, Miltenyi Biotec). The cell suspension was washed twice with PBS. Viability was measured using Vi-cell XR Viability Analyzer (Beckman Coulter). Single-cell RNA-seq was performed with the 10x Genomics Chromium Single Cell 3' Kit (v3.1) according to the standard protocol