GSM5287940: treatment group 2; Mus musculus; RNA-Seq
collect cell samples and add 1mL TRIZO,Lfully cleavage,add 200 μl chloroform and shake tube vigorously for 35s,After incubation on ice for 2min, the supernatant was centrifuged, transferred to a new EP tube, 600 μl isopropyl alcohol and 2  μl  glycogen were added. After full incubation, the supernatant was centrifuged, and the precipitation was cleaned with 70% alcohol. The supernatant was discarded after centrifugation, and the precipitation was dried and dissolved with RNA RNA libraries were prepared for sequencing using standard Illumina protocols