Genomic sequence of Dickeya fangzhongdai AP6: Nanopore reads
Genomic DNA was isolated using the protocol for Gram-negative bacteria using the Gentra Puregene Yeast/Bacteria Kit (Qiagen, U.S.A.)  according to manufacturers protocol with the following modifications: 1 ml of overnight culture was pelleted at ~16,000 x g for 1 minute. DNA pellets were suspended in 50 l 10 mM Tris pH 7.5, incubated at 65C for 1 hour and frozen at -20C. DNA integrity was assessed by agarose gel eletrophoresis.  The isolated DNA was used to prepare a Nanopore sequencing library using the ONT Rapid Barcoding Sequencing kit (SQK-RBK004). The sequencing reaction was run in a MinION flow cell.. Base calling was performed using Guppy v. 6.0.1 (<https://github.com/nanoporetech/pyguppyclient>).