GSM5385592: cvp2cvl1 Rep3; Arabidopsis thaliana; RNA-Seq
50 torpedo staged embryos of each genetic background were manually dissected out of the ovule with needles and immediately placed in TRIzol reagent (Ambion) and grounded with a sterile pestle. All samples were frozen in TRIzol and kept at -80ºC until RNA extraction. RNA extraction was completed by incubating samples at 60ºC for 30 minutes and then purified according to Palovaara J, et al. 2017 (Nature Plants 3(11):894-904). The resulting RNA was cleaned up and concentrated using the RNeasy MinElute Cleanup Kit Qiagen. Extracted RNA was processed into sequencing libraries and sequenced at the FGCZ (https://fgcz.ch/, Switzerland) using the Illumina Novaseq sequencer