GSM5287505: M9; Mus musculus; RNA-Seq
Following paw tissue digestion, live cells were obtained using EasySep Dead cell Removal Kit. Cells were incubated with AF700 CD45 for 30 minutes at 4C. Cells were suspended in FACS buffer and BD FACS Aria II was used to sort for CD45 positive cells, which were collected for single cell sequencing. Single Cell libraries were created using the Chromium 3' Library & Gel Bead Kit as described in the manufacturers user guide.