GSM4980286: glioblastoma cells-NC_1; Homo sapiens; miRNA-Seq
RNA was extracted using the Trizol reagent and rRNA was removed. RNA samples were fragmented into appropriate short fragments. RNA fragments of 18–30 nucleotides in length were enriched by polyacrylamide gel electrophoresis (PAGE). After adding 3' and 5' adapters, samples were subjected to RT-PCR, and PCR products with 140-160 bp size were enriched to generate a cDNA library cDNA libraries were prepared for sequencing using standard Illumina protocols