GSM5334863: N-66; Homo sapiens; miRNA-Seq
Fresh-frozen LUAD tumour and patient matched non-malignant lung parenchymal tissues were collected from treatment naive patients. Tissue microdissection was guided by a lung pathologist to ensure >80% tumor cell or >80% non-malignant cell content. Total RNA was extracted using TRIzol reagent (Life Technologies, Carlsbad, CA, USA/ThermoFisher, MA). Full description of library construction and sequencing are as previously described [TCGA: Comprehensive molecular portraits of human breast tumours. Nature 2012, 490(7418):61-70].