GSM5772193: Prdm16 Het 2; Mus musculus; RNA-Seq
E14.5 cochlear ducts from both inner ears of one embryo were microdissected in DEPC-treated cold PBS, collected in RNA extraction buffer and homogenized (Bel-Art 650000000). RNA extraction and subsequent removal of genomic DNA were performed using RNAqueous RNA isolation kit (Invitrogen, AM1931) as per the manufacturer protocol. Three littermates per genotype were used. RNA libraries were prepared for sequencing using standard Illumina protocols