GSM5573703: ChIP-seq_4_S4_L001; Homo sapiens; ChIP-Seq
Cells were lysed in 1ml LB1 buffer at 4°C for 15 min, and nuclei were pelleted at 2650 rcf for 5 min. Nuclei were resuspended in 1ml LB2 buffer and incubated rotating at 4°C for 10 min and pelleted at 2650 rcf for 5 min. Nuclei were lysed in 1ml LB3 buffer and sonicated using Covaris for 1.5 hours. TruSeq ChIP Library Preparation Kit manufacturer's protocol (Illumina)