GSM5288024: Hippocampus sample from subject 40; Macaca mulatta; RNA-Seq
Genomic DNA and RNA were extracted from each brain region using the All-Prep DNA/RNA/miRNA Universal kit (Qiagen Sciences Inc, Germantown, MD) following the manufacturer's recommendations. Briefly, each brain section was pulverized, and ~30 mg of tissue was used for DNA/RNA isolation. For stranded RNA-seq, cDNA libraries were prepared with a TruSeq stranded mRNA library prep Kit (cat# RS-122-2101, Illumina, San Diego, CA, USA). RNA-Seq: The libraries were sequenced on a HiSeq 4000 (Genomics & Cell Characterization Core Facility, University of Oregon) using a paired-end run (2 × 150 bases).