GSM5335408: 1_Th1_r1; Mus musculus; RNA-Seq
total RNA was prepared from 1 million cells using the mirVana miRNA Isolation Kit (Thermo Fisher Scientific). 1 µg of total RNA was used to prepare the RNA-seq library using the TruSeq SRRNA Sample Prep Kit (Illumina, San Diego, CA, USA), according to the manufacturer's instructions.