WWTP secondary effluent metagenome sequenced by MinION, ONT
Extracted DNA from samples were checked using Qubit and electrophoresis to ensure 1.5 ug DNA (> 3 kbps) for library prep. Library was prepared and sequenced as instructed by the ONT protocol "Native barcoding amplicons (with EXP-NBD104, EXP-NBD114, and SQK-LSK 109)".