GSM5501442: E12.5 DFL Del(CBS1-2) RNAseq rep2; Mus musculus; RNA-Seq
Micro-dissected individual pairs of either control or mutant E12.5 proximal and distal forelimbs were stored at -80˚C in RNAlater stabilization reagent (Ambion) before further sample processing. Total RNA was extracted from tissues using Qiagen RNeasy Plus Micro Kit (Qiagen) after disruption and homogenization according to manufacture instructions. RNA quality was assessed using an Agilent 2100 Bioanalyser. The sequencing libraries were prepared according to TruSeq Stranded mRNA Illumina protocol, with polyA selection. RNA-seq libraries were sequenced on an Illumina HiSeq 4000 sequencer, as single-end reads (read length 50 bp).