GSM5384445: Conditioned control 2 ATAC-seq; Mus musculus; ATAC-seq
Frozen brain septum punches were extracted in 500 µl nuclear isolation buffer (10 mM Tris, 10 mM NaCl, 3 mM MgCl2, 0.1 % Igepal, 0.1 % Tween, protease inhibitor cocktail). Single cell separation was performed using a 21 G needle on a 1 ml syringe to shear the tissue through the needle 5 times. NeuN-positive nuclei were separated by fluorescence-activated cell sorting. ATAC-seq was performed as previously described by Buenrostro et al., 2013