RNAseq of paratransgenic Gmm: midgut
All flies were dissected 36 h after the third blood meal; 10 individual cardia or 5 individual midgut were pooled as one biological replicate and stored in -80oC prior to RNA extraction, a total 3 biological replicates per treatment were used. Total RNA was extracted using Trizol reagent according to the manufacturers protocol (Invitrogen), followed by RNA Clean and Concentrator Kit and in-column DNase treatment (Zymo Research). RNA quality and quantity were quantified using a bioanalyzer. All 6 mRNA libraries were prepared and sequenced (pair-ended) at Yale Center for Genome Analysis (YCGA) using Illumina NovaSeq system