GSM5501081: scATACseq_vehicle; Homo sapiens; ATAC-seq
Cell were lysed with  ATAC-RSB buffer (Tris-HCl pH 7.4 10mM, NaCl 10mM, MgCl2 3mM, NP-40 0.1%, Tween-20 0.1% and Digitonin 0.01%) and underwent transposase reaction. Tagmented DNA was isolated with Zymo DNA Clean and Concentrator-5 kit (Zymo Research). Tagmented-DNA was amplified and barcoded by PCR to construct DNA library. DNA fragments underwent a doubled-side size selection to remove primer dimers and fragments larger than 1000bp