GSM4982208: At Col-0 CO-7; Arabidopsis thaliana; RNA-Seq
Thirty seedlings (entire ornisms) from each replicate were harvested and flash frozen using liquid nitrogen, then pulvarized using a mortar and a pestle. RNA extraction was performed using the standard Trizol method. RNA was purified using RNeasy RNA-Extraction Kit (Qiagen) following rhe manufacturer's protocols. 1 µg of RNA was used to construct libraries using the TruSeq  Stranded mRNA Sample Preparation Kit (Illumina). RNA libraries were constructed with TruSeq Stranded mRNA Sample Preparation Kit (Illumina) following the protocol provided by Illumina. RNA-seq libraries were purified, amplified and sigle-end sequenced (100-nt length) on two flow cells of Illuimna HiSeq2500 Sequencer at the Genome Sequencing Facility of the University of Kansas Medical Center.