GSM5381736: JLYZ0036 [WGBS]; Homo sapiens; Bisulfite-Seq
The placental samples were frozen within 4 hours after birth. DNA was extracted from placenta tissue with the Gentra Puregene kit (Qiagen, Hilden, Germany) and quantified with the Qubit DNA Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). DNA was bisulfite converted with the EZ DNA Methylation Lightning kit (Zymo, Irvine, CA, USA). WGBS libraries were prepared from bisulfite-converted DNA using the TruSeq DNA Methylation kit (Illumina, San Diego, CA, USA) with indexed PCR primers and a 14 cycle PCR programs.