GSM5770729: DLD1-USP7KO-Day9-Dox-Rep3; Homo sapiens; RNA-Seq
Each biological triplicate of DLD1 wt, DLD1-USP7-KO and DLD1-KO-Dox-USP7 were grown in a 10 cm2 plate. USP7 expression in DLD1-KO-Dox-USP7 cells was induced by the   GFP-USP7 expression was induced by addition of doxycycline  (1 µg/ml) for 24 hours, three days or 9 days (+Dox). For RNA isolation the medium was removed and 3 ml of TriPure isolation reagent (Roche) was added directly onto the cells. Samples were prepped with the Illumina TruSeq Stranded mRNA Library Prep Kit. The resulting DNA libraries were sequenced according to the Illumina Truseq Rapid V2 protocol on an Illumina HiSeq2500 sequencer.