GSM5833271: PBMC-01-3-GEX-S1; Homo sapiens; RNA-Seq
A pooled suspension containing 2,000,000 thawed PBMCs from at most 20 subjects (~100,000 per subject) were stained with 282 Totalseq antibodies according to the Totalseq-A protocol (https://www.biolegend.com/en-us/protocols/totalseq-a-antibodies-and-cell-hashing-with-10x-single-cell-3-reagent-kit-v3-3-1-protocol). Stained cells were then loaded onto the Chromium Controller at an input density of 2,500 cells/µL and cDNA libraries were prepared using the 10X Genomics Single Cell 3' v3.1 Assay. Peripheral blood was collected from each subject in Vacutainer ACD tubes. PBMCs were isolated using a standard Ficoll method and stored in liquid nitrogen.