cyPhyRNA-seq of Desulfovibrio vulgaris
This approach employs, among other techniques, the specific ligation-based capture of ribozyme 5-fragments with 2',3-cP ends using a variant of the Arabidopsis thaliana tRNA ligase we engineered. To capture ribozyme 3-fragments, they are enriched from total RNA by degrading RNAs with 5-PPP and 5-P.