GSM5288221: Ctr_WildTypeSarA; Staphylococcus aureus; ChIP-Seq
Cells were treated with 1% formaldehyde and lysed by sonication, followed by cytosolic fraction harvesting, and DNA sheering by sonication for an fragment size between 200-500 bp. Followed by extraction, decrosslinking, and purification of DNA. Three replicates were pooled for further analysis. Libraries were prepared according to Illumina's  DNA Sample Kit. 50 ng of total DNA was used for the library preparation and the libraries were pooled at equimolarity and loaded at 2 nM for clustering.