Sequencing negative control in human oral microbiome study
Amplification of V3-V4 region of 16S rRNA gene according to the Illumina MiSeq's protocol. Amplification with KAPA HiFi HotStart ReadyMix PCR kit. Amplicons mixed with Illumina sequencing adaptors and dual-index barcodes (Nextera XT index kit v2, FC-131-2001).