RNA-Seq of Proteus sp.
After batch culturing at 30C for 14 h, YC801 cells were collected and immediately quenched in liquid nitrogen for 5 min, and then stored at -80 C. A total of six samples processed under the same culture conditions were prepared from culture broth without Na2SeO3 (control) and supplemented with 5 mM Na2SeO3 (selenite-treatment). The samples were defined as CK-1, CK-2, CK-3, Se-1, Se-2 and Se-3, respectively. The total RNA extraction, assessment of RNA integrity and construction of cDNA library were conducted in Beijing Genomics Institute (BGI, Shenzhen, China) according to the methods described by Wang et al[43]. Finally, the sequencing of the cDNA library was performed by using an BGISEQ-500 platform (BGI, Shenzhen, China) according to the manufacturer's standard procedure[44]. After the quality control, raw reads were filtered to obtain high-quality clean reads by removing adaptor sequences, low-quality sequences, or other unknown technical sequences. The clean reads were then mapped to the reference genome sequences of P. mirabilis ATCC 29906 (Genome assembly ASM16075v1).