GSM5994280: MPWT2; Mus musculus; ATAC-seq
Sorted LT-HSCs and MPPs were tagmented using Tn5 (Illumina) Libraries were prepared according to Buenrostro et al. (2015, Curr Protoc Mol Biol) with minor modifications. Briefly, nuclei DNA was tagmented and extracted. Then, a size selection (0.65x) was applied. Libraries were barcoded with Illumina sequencing indexes. Sequencing was performed on 2×50 HiSeq 3000 (Illumina) and with an average of 25 million paired-end reads per sample.