Microbial-only reads from matched healthy control: Sample H16
Total RNA isolated from microdissected tumor/normal tongue cancer tissue pairs or tongue scrapings form healthy control using All prep micro Kit ( Qiagen 80284). Ribosomal RNA depleted using 2:1 cocktail of Human and bacterial rRNA depletion probes (NEB kits E7400 & E7850). Depleted RNA was used to prepare libraries using NEBNext® Ultra™ II Directional RNA Library Prep kit (E7765).