GSM4981512: 415B_TNF: Hi-C, Primary FLS, TNF stimulated; Homo sapiens; Hi-C
Cultured human FLS from RA patients were treated with 10 ng/ml TNF for 24 h or were left untreated. Cells (1e7 to 3e7 per condition), were scratched in 10ml DMEM, spun down, suspended in 35ml DMEM and fixed (2% formaldehyde in DMEM, 10min, RT, with mixing on a rocker). The reaction was quenched with cold 0.125M glycine. Cells were incubated at RT for 5 min, followed by 15 min incubation on ice and centrifugation (1500 rpm, 10 min, 4 ⁰C). Pellets were suspended and washed in cold PBS (1500 rpm, 10 min, 4 ⁰C). Washed pellets were snap frozen and stored at -80 ⁰C. Hi-C libraries from RA FLS samples, were generated as previously described in Lieberman-Aiden, E. et al.