RNA-Seq of Proteus sp.
After batch culturing at 30C for 14 h, YC801 cells were collected and immediately quenched in liquid nitrogen for 5 min, and then stored at -80 C. A total of six samples processed under the same culture conditions were prepared from culture broth without Na2SeO3 (control) and supplemented with 5 mM Na2SeO3 (selenite-treatment). The sam-ples were defined as CK-1, CK-2, CK-3, Se-1, Se-2 and Se-3, respectively. The total RNA ex-traction, assessment of RNA integrity and construction of cDNA library were conducted in Beijing Genomics Institute (BGI, Shenzhen, China) according to the methods described by Wang et al[43]. Finally, the sequencing of the cDNA library was performed by using an BGISEQ-500 platform (BGI, Shenzhen, China) according to the manufacturer's standard procedure[44]. After the quality control, raw reads were filtered to obtain high-quality clean reads by removing adaptor sequences, low-quality sequences, or other unknown technical sequences. The clean reads were then mapped to the reference genome sequenc-es of P. mirabilis ATCC 29906 (Genome assembly ASM16075v1).