GSM5834262: WT.d2320 [CTL rep 1]; Mus musculus; RNA-Seq
RNA was extracted from  >10,000 Dct-GFP+ sortedf cells with TRIzol using standard phenol chloroform extraction and then purified using the Qiagen RNeasy Micro Kit following the manufacturer's protocol. The quality and quantity of RNA samples were determined using an Agilent Femto Pulse. RNA-seq libraries were prepared from 1 ng of total RNA using a TaKaRa SMARTer Stranded Total RNASeq Kit v2 Pico Input Mammalian kit with a 4-min fragmentation and 15 cycles of PCR. After cDNA cleanup, samples were treated with ZapR reagent and library fragments not cleaved by ZapR were further amplified by 15 cycles of PCR. Illumina libraries were validated using the Agilent Fragment Analyzer and quantified by qPCR