GSM5833506: TAOADIPOSE1123; Homo sapiens; RNA-Seq
Following Gel Beads-in-emulsion (GEMs) generation, the Gel Bead is dissolved, primers are released, and any copartitioned cell is lysed. Incubation of the GEMs produces barcoded, full-length cDNA from poly-adenylated mRNA. RNA was then reverse-transcribed into cDNA libraries using a 10x Genomics Chromium machine following the manufacturer's instructions. DNA sequencing was performed on a NovaSeq 6000 Sequencing System (Illumina, CA, USA) using a paired-end 150 sequencing mode.