GSM4851825: 1268 myo primary control RNA-seq; Homo sapiens; RNA-Seq
Cells were harvested for RNA using RNeasy mini kit (Qiagen, 74104). TruSeq stranded mRNA (Illumina, 20020594) kit was used for the preparation of libraries for RNA-seq. From 100ng of the total RNA, mRNA was purified and fragmented using the reagents provided with the kit. Blunt ends of cDNA were adenylated at 3' end, adapters ligated, and library amplified. 2nM libraries were pooled, quantified and sequenced as paired end using Nextseq 500/550 high output kit (v2, 75 cycles).