GSM5213481: Med15-AID, +DMSO, rep-3; Saccharomyces cerevisiae W303; OTHER
Cells were labelled with 4tU for 6 minutes. Total RNA was extracted using the Masterpure Yeast RNA extraction kit (Lucigen MPY03100) as per the manufacturer's protocol. Total RNA was subjected to biotinylation, pull-down and purification as previously described in Donczew et al, 2020 [PMID:31913117]. rRNA was depleted from the purified nascent RNA fraction using Terminator™ 5′-Phosphate-Dependent Exonuclease (Lucigen TER51020) as per the manufacturer's protocol. rRNA-depleted nascent RNA was purified and concentrated using RNAClean XP clean beads (1.8:1 beads:sample ratio). TruSeq Stranded Total RNA kit for Illumina