GSM5287582: U87-input DNA; Homo sapiens; ChIP-Seq
Lysates were clarified from sonicated nuclei and RNF2-DNA complexes were isolated with antibody. The libraries were constructed following NEB's Chip-seq Sample prep kit. Briefly, Chip DNA was end-blunted and added with an 'A' base so the adaptors from NEB with a 'T' can ligate on the ends. Then 200–400 bp fragments are double-selected and purified. The library was amplified by 18 cycles of PCR.