HEK293T-NC_WGS
plasmid pSB-CRISPR-sgNC  was transfected together with the SB100X plasmids into HEK293T cells.  The cells were then kept in the presence of puromycin, forcing the Cas9 endonuclease and sgRNAs to express constantly and keep cleaving the chromosomes. The cells that survived after puromycin selection were designated as SCR-NC