GSM5145665: A03- FAC replicate 03; Homo sapiens; RNA-Seq
Total RNA was extracted using a RNeasy Micro kit (Qiagen #74004) following the manufacturer's instructions Libraries for RNA-Seq were prepared with Kapa Stranded Kit. The workflow consisted of mRNA capture, cDNA generation, and end repair to generate blunt ends, A-tailing, adaptor ligation and PCR amplification.