PacBio Hifi of Hesperophylax magnus
Single adult specimens were flash frozen and DNA was extracted. Total genomic DNA was quantified using Qubit 3.0 Fluorometer (Life Technologies Q33216) and the dsDNA BR Assay Kit (Life Technologies Q32854). Sample purity was assessed using Nanodrop. Extractions with at least 1 ug of high-molecular weight ( > 40kb) were sheared and the BluePippin system (Sage Science)  was used to collect fractions containing 20 kb fragments for library preparation. Sequencing libraries were prepared for each species using the SMRTbell Express Template Prep Kit 2.0. Each library was run separately for 30 hrs on two 8M SMRTcell using the PacBio Sequel II system. All lab work was carried out at DNA Sequencing Center at Brigham Young University (Provo, Utah, USA).