DNA-Seq of hybryd animal: Bactrian camel * Dromedary camel
For sequencing, after shearing of genomic DNA, fragments with 300-500bp were generated and in the next step, these fragments were repaired, A-tailed, and ligated to Illumina sequencing adapters. DNA fragments with 370-470bp were selected on 2% agarose gels and after amplification using PCR, were used for sequencing. Finally, one lane of 150bp paired-end sequencing was performed by Illumina HiSeq 2500 system.