GSM5745248: ME5; Homo sapiens; RNA-Seq
Total RNA from UL and MM tissues (n= 30) was extracted with TRIzol reagent (Fisher Scientific, Waltham, MA). Samples were quantified using TapeStation 4200 (Agilent, USA). cDNA synthesis and library generation were performed using TruSeq RNA Library Prep Kit v2 (Illumina, USA) according to manufacturer's instructions. Samples were quantified using Bioanalyzer (Agilent, USA) and DNA pools generated were quantified using Qubit DNA HS assay kit (ThermoFisher Scientific, Waltham, MA, USA). Libraries were sequenced using Illumina NovaSeq 600 Instrument (Illumina, USA) with a in a paired-end design. RNA libraries were prepared for sequencing using standard Illumina protocols