GSM5452276: WT_IL33_24h_A; Mus musculus; RNA-Seq
Total RNA was extracted using the MagMAX mirVana Total RNA Isolation Kit (Applied Biosystems) as per the manufacturer's protocol. RNA integrity was assessed using a Bioanalyzer RNA Pico kit (Agilent). Ribosomal RNAs were depleted and libraries were prepared with the KAPA stranded total RNA kit (Roche), then multiplexed and sequenced on an Illumina HiSeq 2500 flow cell in a paired-end 50bp configuration. rRNA-depleted stranded RNA-Seq