WES of AFB1 treated male rat liver
Frozen, tissue cubes from the median liver lobe of each animal were lyophilized to enhance capture of the heterogenous nature of liver tissue. DNA (gDNA) was isolated from 10 mg of the respective frozen, lyophilized sample according to manufacturers instructions (Zymed Duet DNA/RNA MiniPrep Plus Kits, Zymo Research, Irvine, CA). Sample integrity and yield were assessed by the Nanodrop (Thermo Fisher Scientific, Madison, WI), the Qubit Fluorometer (Thermo Fisher Scientific) and the 5200 Fragment Analyzer System, (Agilent Inc., Santa Clara, CA). Purified gDNA samples were stored at -20 C. Sequencing libraries were prepared using the Agilent SureSelectXT method. Following fragmentation, post-capture library amplifications and quality assessments of exome enriched samples (SureSelect CD Rat All Exon Kit, Agilent) were sequenced, (125 bp paired-end read) on the Illumina HiSeq2500 (v4, San Diego, CA).