GSM5906356: Input_CPT1a_KO+Ac-3; Mus musculus; ChIP-Seq
spheroids were cross-linked with 1% formaldehyde and then quenched by addition of glycine. Nuclei were isolated and resuspended in L3B+ buffer (10 mM Tris-Cl pH 8.0, 100 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 0.1% sodium deoxycholate, 0.5% N-Lauroylsarcosine, 0.2% SDS). Chromatin was fragmented to 100-300 bp using 25 cycles (30 sec on, 30 sec off, 20% amplitude) using Qsonica Q800R. Chromatin immunoprecipitation was performed overnight in the presence of H3K9ac antibody (Cell signaling, 5685S) conjugated to magnetic protein A/G beads all libraries were prepared for sequencing using standard Illumina protocols