GSM5145496: FA8mg_Poly.1; Mus musculus; RNA-Seq
Cells were microdissected from paraffin-embedded tissues usig Leica LMD6500 system.  RNA was isolated using the RecoverAllTM total Nucleic Acid Isolation for FFPE kit.  RNA concentration and quality were determined on an Agilent 2100 Bioanalyzer using RNA 6000 Pico chips. Libraries were generated using SMARTerÒ Stranded RNA-Seq Kit V2- Pico Input Mammalian with 40 ng total RNA. Libraries were quantified using a Qubit ds DNA HS kit and size distribution validated on the Agilent 2100. Bioanalyzer using the Agilent High Sensitivity DNA kit.