RNA-seq of mus musculus
Limited RNAmore than 200pg,high-qualitywas amplified with oligo-dT and dNTPs,incubatedat 72and immediately put back on ice,thenreverse transcribed tocDNA based onpolyA tail.Thetemplate wasswitchedto the5' end of the RNA and the full-length cDNAwasgeneratedby PCR