GSM4852397: Patient 15; Homo sapiens; Human papillomavirus type 16; RNA-Seq
RNA was extracted from snap-frozen tissue of 4 patients (Maxwell® RSC simplyRNA Tissue Kit(AS1340), Promega, Southampton, UK). RNA concentration and quality were analyzed using the RNA Nano Kit for the 2100 Bioanalyzer System (Agilent Technologies, Santa Clara, CA, USA). RNA-sequencing was undertaken by Edinburgh Genomics (University of Edinburgh, Edinburgh, UK). An automated TruSeq stranded mRNA-seq library preparation from total RNA and the 137 UK). An automated TruSeq stranded mRNA-seq library preparation from total RNA and the NovaSeq sequencing-system was used (100bp paired-end; 1,750M+1,750M reads, Illumina, San Diego, CA, USA).