GSM5835329: RNA-seq_jmj26_Rep1; Arabidopsis thaliana; RNA-Seq
Total RNA was extracted using ZR Plant MiniPrep Kit (Zymo Research) including a DNase I treatment and was quantified by fluorometer Qubit 4 (Invitrogen). Total RNA was treated with the poly(A) RNA Selection Kit (Lexogen).　 Libraries were prepared according to the manufacturer's protocol in NEBNext Ultra II Directional RNA Library Prep Kit (New England BioLabs) and individually indexed with NEBNext Multiplex Oligos for Illumina (New England BioLabs). The quantity and quality of each amplified library were analyzed by using Fragment Analyzer (Agilent) and HS NGS Fragment Kit (Agilent).