16S rRNA V4 gene amplicon sequencing of Lake Vrana 10, particle-associated -3um, March (515F/806R) [JMF-2112-11-0010A]
Amplicon sequencing of Lake Vrana 10, particle-associated -3um, March. Water samples were taken at 1 m depth with a UWITEC water sampler, except then the water level was too low, in which cases the samples were taken at lower depths to avoid the sediment bottom. Two liters of the water column sample were carried in the PC bottles and sequentially filtered onto 3 um and 0.2 um polycarbonate (PC) filters (Whatman Nuclepore Track-Etch Membrane, diam. 47 mm). Filters were stored at -80 C until further processing. Genomic DNA from filters was extracted using the DNeasy Power Water Kit (Qiagen, Germany) following the provided protocol. The V4 hypervariable region of the 16S rRNA gene was amplified and sequenced as described in https://doi.org/10.3389/fmicb.2021.669776. {primers: GTGYCAGCMGCCGCGGTAA + GGACTACNVGGGTWTCTAAT, nucleic acid: DNA, flow cell identifier: JV829, barcoding: UDB-H12, plate-position: 5 G3, barcodes: CTGGGAGTTGTT + CTGGATTACGGT}.