GSM5216340: mesenchymal stem/progenitor cells of cKO mouse rep2 [31]; Mus musculus; RNA-Seq
Bone marrow cells were digested and harvested. Stromal cells were enriched by depleting hematopoietic cells with biotin-conjugated CD45 and Ter119 antibodies, and Dynabeads® Biotin Binder (Invitrogen #11047). CD45- Ter119- CD31- CD144- PDGFRα+ SCF-GFP+ cells were sorted into 350 μL RLT plus buffer with 3.5 μL β-mercaptoethanol. RNA was extracted using the RNeasy® Plus Micro Kit (Qiagen #74034). Libraries were prepared after Poly A selection