16S rRNA gene-based analysis of the bacterial community of sample 13 from Mix material with DNA extraction method PCB of cow A
Metagenomic DNA was extracted from the Mixed ruminal fraction of Cow A, using the Phenol Chloroform Bead beating method. The V3-V4 region of 16S rRNA genes was amplified. All samples were amplified in triplicate, purified and pooled in equal amounts. Sequences were determined using an Illumina MiSeq sequencer.