Nanopore MinION sequencing of Salmonella enterica strain 2015-006 isolated from contaminated flax seeds
Strain grown 24h at 35C on TSA plate and 10uL loopful of cells was resuspended in 800uL Zymo DNA/RNA Shield. DNA extracted using Zymo Quick-HMW kit and RNAseA treatment. DNA was processed (SPRI selection >1500bp). Rapid barcoding sequencing kit (SQK-RBK-004) and 1D MinION sequencing using FLO-MIN106 R9.4 flowcell.