GSM5066802: MEF exo; Mus musculus; miRNA-Seq
The beating colonies were picked at designated day and total RNA was isolated using Trizol reagent (Thermo Scientific). RNA quality was assessed by the RNA 6000 Pico Chip for exosomal miRNA sequencing (Agilent Technologies). NEBNext Multiplex Small RNA Library Prep kit (New England BioLabs) was used for exosomal miRNA sequencing according to the manufacturer's instructions. exosomal microRNA sequencing