18S rRNA gene-based analysis of the eukterial community of sample 3 from Mix material with DNA extraction method QSB of cow A
Metagenomic DNA was extracted from the Mixed ruminal fraction of Cow A, using the Qiagen Soil kit Bead beating extraction method. The V4 rRNA loop of 18S rRNA genes was amplified. All samples were amplified in triplicate, purified and pooled in equal amounts. Sequences were determined using an Illumina MiSeq sequencer.