GSM4980180: Subject RC30, household contact control; Homo sapiens; RNA-Seq
Whole blood was collected in PAXgene tubes and frozen. RNA was extracted automatically using a QIAcube with the PAXgene Blood miRNA Kit. Libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7765). Libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7765) including poly(A) enrichment. Additionally, globin reduction was performed using GLOBINclear kit (Cat#AM1980). Briefly, mRNA was isolated from total RNA using the oligo-dT magnetic beads. After fragmentation of the mRNA cDNA was synthesized. This was used for ligation with the sequencing adapters and PCR amplification of the resulting product. Libraries were sequenced on the Illumina NovaSeq6000 System with a 150bp paired-end protocol.