GSM4981552: 430_TNF: RNA-seq, Primary FLS, TNF stimulated; Homo sapiens; RNA-Seq
Cultured FLS were treated with 10 ng/ml human recombinant TNF (Roche) for 24 h or were left untreated. Total RNA was isolated using the RNeasy Mini kit (Qiagen) including on-column DNAase I digestion. RNA-seq libraries were generated using the Illumina TruSeq Stranded total RNA protocol with the TruSeq Stranded total RNA Sample Preparation Kit