Amplicon sequencing of SARS-CoV-2 from wastewater
SARS-CoV-2 amplicons of 400-bp were generated according to the Freed et al. 2020 protocol, with the described primer set replaced with those from the ArticV3 protocol. Amplicons were library prepped using the Oxford Nanopore Rapid Barcoding Kit and sequenced on a Flongle flow cell (FLO-FLG001) on a Mk2b MinION sequencer.