GSM5335129: Vv_Yeast_12h_rep2; Vitis vinifera; RNA-Seq
For short-read RNA-seq, total RNA of harvested embryogenic callus were isolated according to the manufacturer's instructions of Spectrum Plant Total RNA Kit (Sigma). Each purified RNA sample was treated with DNase I following the TURBO DNA-free kit protocol (Ambion) to remove contaminating genomic DNA. For long-read sequencing, Total RNA was extracted and separated using NORGEN Plant microRNA purification kit (Norgen Biotek) according to the manufacturer's instruction. Genomic DNA contamination was removed with the standard protocol of the TURBO DNA-free kit (Thermo Fisher). For short-read RNA-seq, RNA libraries were prepared using standard Illumina protocols. For long-read cDNA-seq, the libraries were prepared following the protocol of Oxford Nanopore cDNA-PCR kit (SQK-PCS109).