RNA-Seq of mus musculus: adult male bone marrow derived macrophages
mRNA molecules were purified from total RNA using oligoDT magnetic beads and then fragmented. First and second strand cDNA were synthesized, ends were repaired, and A base was added to 3' ends. A linker was connected to the A base, and PCR amplification was done using UDG enzyme. PCR products were purified and the library was validated via an Agilent Technologies 2100 bioanalyzer. PCR products were circularized and sequenced.