GSM5573527: 14 Ly6C low; Mus musculus; RNA-Seq
Non-parenchymal cells were isolated from mice liver and Ly6C high and Ly6C low macrophages were sorted by flow cytometry cell sorter (BD FACSAria III) in RLT lysis buffer. RNAs were isolated using RNeasy Microkit (QIAgen, #74004). RNA libraries were prepared for sequencing using NEBNext Single Cell/Low Input RNA Library Prep from New england Biolabs. cDNA and final libraries were controlled with AGILENT Tapestation 2200 on High Sensitivity DNA chip. Final equimolar pooled library prep were sequenced on Nextseq 500 ILLUMINA with HighOutPut cartridge (2x400Millions of 75 bases reads). Corresponding to 2x20Millions of reads per sample after demultiplexing.