RNA seq of MSMEG_2295 knockout
Total 100 ng of ribodepleted RNA was utilized and converted into the final library using Truseq stranded RNA library preparation kit following manufacturer's protocol (Illumina, USA). Minimum 8 million high-quality paired-end reads were generated from each sample. Raw reads were trimmed for adapter removal, and high-quality reads were utilized for the differential gene expression calculations using the DESeq2 tool.