pep1_7wLD_R1
Total RNA was extracted from apices using the RNeasy Plant Mini Kit (Qiagen) and treated with DNA-free DNase (Ambion). PolyA RNA was isolated from 1 g of total RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Frankfurt am Main, Germany) and used for library construction with NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs).