GSM4982073: MDcell-LS+ACEidiet-rep1; Mus musculus; RNA-Seq
MD cells (green) and control cells (intense red) were isolated by FACS. Cells were extracted using Qiagen miRNeasy purification kit following manufacturer's protocol for total RNA purification (Qiagen cat#217004)MD-GFP mice on C57BL6/J background were generated by intercrossing nNOS/CreERT2 and mTmG/fl mice resulting in membrane-targeted eGFP expression in MD cells (green), and membrane-targeted tdTomato expression in all other kidney cell types (red). The highest tdTomato expressing cells were collected as controls representing epithelial cells from distal tubule-collecting duct tubule segments. Libraries were simultaneously prepared using Takara's SMARTer Stranded Total-RNA Pico v2 library preparation kit following manufacturer's protocol (Takara cat#634412).