GSM5726448: m1VNO; Mus musculus; RNA-Seq
8-week-old C57BL/6J mice were flushed of blood with a heparin solution under anesthesia. Vomeronasal epithelia were isolated and transferred to a tube containing 500–600 μL of ice-cold lysis buffer, 5–6 μL β-mercaptoethanol and a 0.5-cm diameter steel ball, and then put on ice, following the manufacturer's protocol (Qiagen). Samples were then homogenized using a FastPrep-24 instrument (MP Biomedicals) at 6 ms−1 for 30 s and kept on ice. RNA was extracted using the Qiagen RNeasy Mini kit SII following the manufacturers' instructions. Two DNase treatments were performed on all samples: first using the Qiagen RNase-Free DNase Set and then using the Life Technologies Ambion DNase I kit. RNA samples were then aliquoted and stored at −80 °C. cDNA libraries were generated with the TruSeq RNA and DNA sample preparation kits after selection of polyA-containing mRNAs. Adapters for RNA-seq multiplexing were added to the cDNAs. The cDNA libraries were sequenced with a HiSeq2500 Sequencing system to generate stranded single-end 100 reads of 100 nucleotides length.