GSM6199125: W2,control,Day8; Drosophila melanogaster; RNA-Seq
80 Malpighian tubules of each genotype were lysed with Trizol (ThermoFisher, 15596018) for RNA extraction. After assessing RNA quality with Agilent Bioanalyzer (RIN > 7), mRNAs were enriched by poly-A pull-down. Sequencing libraries were prepared with Illumina Truseq RNA preparation kits and were sequenced using Illumina HiSeq 2000 by Benagen (Wuhan, China).