16S sequencing of Magallana gigas: 2 days post fertilization
Total DNA was sent to the Integrated Microbiome Resource at Dalhousie University (Halifax, Nova Scotia), for PCR amplification and sequencing. Sequencing libraries were prepared by targeting the V4-V5 regions of the 16S rRNA gene for amplification using the the 515F (GTGYCAGCMGCCGCGGTAA) and 926R (CCGYCAATTYMTTTRAGTTT) primer set.