GSM6004938: SC-EC, +ifn, biol rep 1; Homo sapiens; RNA-Seq
Total RNA was extracted from cell pellet samples using RNeasy Plus Mini Kit (Qiagen, Germantown, MD, USA) and quantified using Qubit Fluorometer (Life Technologies, Carlsbad, CA, USA). SMART-Seq v4 Ultra Low Input Kit for Sequencing was used for full-length cDNA synthesis and amplification (Clontech, Mountain View, CA), and Illumina Nextera XT library was used for sequencing library preparation. Multiplexed sequencing libraries were loaded on the Illumina HiSeq instrument and sequenced using a 2x150 Paired End (PE) configuration.