GSM4982790: HNSCC WT 6-10B cells; Homo sapiens; RNA-Seq
According to the manufacturer's protocol, total RNA extracted from knockout and wildtype cells were reverse transcripted to cDNA by All‐in‐One First‐Strand cDNA Synthesis kit (GeneCopoeia, USA). MRNA as template, synthesised single-stranded cDNA with six bases random primers (random hexamers), then added the buffer, dNTPs and DNA polymerase I, synthesised double-stranded cDNA. Then purified double-stranded cDNA with AMPure XP beads. Purified double-stranded cDNA sequence was repaired the end, added A tail and connected joint of sequencing, and then chose fragment size with AMPure XP beads. Finally got cDNA library after PCR enrichment.