GSM6199347: RAW-WT, HSV-1-4h-1, ATAC; Mus musculus; ATAC-seq
ATAC-seq was performed as previously reported (Buenrostro et al. 2013;Corces et al. 2017; Bajic M et al. 2018). Briefly, nuclei was extracted from samples, and the nuclei pellet was resuspended in the Tn5 transposase reaction mix. The transposition reaction was incubated at 37°C for 30 min. Equimolar Adapter1 and Adatper 2 were added after transposition, PCR was then performed to amplify the library. After the PCR reaction, libraries were purified with the AMPure beads and library quality was assessed with Qubit