GSM6138599: sc_kd_3d; Schmidtea mediterranea; RNA-Seq
For single cell RNA-seq from 10x Chromium platform, Hoechst-stained and PI negative cells (200,000 cells) from wild-type animals, and from cells from wtap knockdown animals were collected on ice using Influx sorter. Approximately 9000 counted cells were loaded per channel. The libraries were made using the Chromium platform and Chromium Single Cell 3' v3 chemistry. Sequencing libraries were loaded on an Illumina nova 6000 flowcell with two 150bp paired-end kits.