GSM6217101: bone marrow,HFD,rep1; Mus musculus; RNA-Seq
Tissue was homogenized with an RNase-free disposable pellet pestle (Fisher) and RNA was extracted using the commercially available RNeasy Lipid Tissue Mini Kit (Qiagen, Valencia, CA). Purification of the isolated RNA included treatment with DNase I (Qiagen, Valencia, CA) RNA libraries were prepared for sequencing using standard Illumina protocols