GSM5452182: D05_3CI; Mus musculus; RNA-Seq
Dissociated organoids in TrypLE. Dissociated cells were filtered through 40µM cell strainer follwed by three washes in 2% BSA/DPBS. Finally, suspended in 2%BSA/DPBS. Single cell 3' RNA-seq experiment is conducted using the Chromium single cell system (10x Genomics, Inc) and the NovaSeq 6000 sequencer (Illumina, Inc).