Illumina NovaSeq 6000 sequencing; RNA-seq of early responses to ASFV
RNA-seq of early responses to ASFV
A total of 30 male and female Large White domestic pigs, 10 to 11 weeks-old and with a body weight of approximately 20-25 kg were obtained from the IVI specific pathogen-free (SPF) breeding facility or a local farm. The SPF and farm pigs were moved to the BSL-3Ag containment facilities and randomly assigned to separate groups of six or three animals, for virus- or mock-infection respectively. During the trial, the animals were fed a commercial pig pellet diet (Granovit AG, Kaiseraugst, Switzerland) with hay supplementation and water ad libitum. After 5 days of acclimatization in the BSL-3Ag containment, the pigs were infected by intramuscular injection of 2 ml DMEM containing ⁓3 to 6x102 TCID50/ml of either the highly virulent Armenia 2008, or the naturally attenuated Estonia 2014 strain. Mock-infected animals received 2 ml of DMEM. Blood samples were collected 1-3 days prior to infection and on 1, 2, 4, 5, 7, 9, 11, 14, 21 and 26 days post-infection, or until discontinuation criteria were reached.  Whole blood RNA extraction whole blood kit Lexogen QuantSeq FWD