GSM5835124: hFFs, siATRX, rep2; Homo sapiens; ATAC-seq
Isolation of nuclei was carried out by Diagenode SA (Seraing (Ougrée), Belgium) utilizing the ATAC-seq service (Assay for Transposase-Accessible Chromatin) (Diagenode Cat# G02060000). Nuclei were isolated with a concentration of 0.4% of Igepal CA-630 in the lysis buffer. Library preparation was carried out by Diagenode SA (Seraing (Ougrée), Belgium) utilizing the ATAC-seq service (Assay for Transposase-Accessible Chromatin) (Diagenode Cat# G02060000). Lysed nuclei were transposed for 30 minutes at 37°C using the prokaryotic Tn5 transposase system (Nextera DNA library kit, Illumina, FC-121–1030), purified on Diapure columns (Diagenode, C03040001), and libraries were prepared using NEBNext High-Fidelity PCR MasterMix (NEB, M0541) and Illumina indexing primers