GSM6081169: Input DNA [WT_3]; Mus musculus; ChIP-Seq
1 million FACS-purified SMCs were crosslinked upon the instruction of Covaris kit (#520154). 20 µg sheared genomic DNA was subjected to immunoprecipitation with 4 µg H3K36me3 antibody according to the established protocol. 0.5-10ng of DNA was used as input for TruSeq ChIP Library Preparation Kit (Illumina) with size selection by SPRI-bead based approach after final PCR with 18 cycles.