GSM5288649: 4T1 cells, CHD8_rep2 (ATACseq); Mus musculus; ATAC-seq
ATACseq assay were performed using Illumina kits. Briefly, a homogenous cell pellet was lysed and resuspended in a transposition mix. The samples were then incubated at 37deg for 30mins and then purified using the Qiagen MinElute PCR Purification Kit. Eluted sample were then processed for library prep and sequencing. Libraries were prepared using the PCR steps (12 cycles) in the Illumina Nextera DNA library preparation kit. The pool was run as a PE150 run type on one lane of a HiSeq 4000. ATACseq, PE150