RNA-Seq of bermudagrass: stolon
Total RNA was extracted from the frozen organ samples using RNAprep pure Plant kit (Tiangen, Beijing, China). RNA integrity and concentration were determined by gel electrophoresis and Nanodrop 2000 spectrophotometer (Thermo Scientific, Wilmington, USA), respectively. Only samples of high-quality RNA (RNA integrity number  7) were used for following library preparation. cDNA libraries were prepared using Illumina TruSeq RNA Sample Preparation Kit (Illumina, San Diego, USA) according to the manufacturers instructions. Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, USA) was used to check the quality of the libraries. The qualified cDNA libraries were paired-end sequenced using Illumina HiSeqTM 3000 (Illumina, USA). Each samples were sequenced with three biological replicates.