GSM5772177: 4T1_BF10-3; Mus musculus; RNA-Seq
Trizol protocol for tissue RNA extraction. Extracted RNA were quantificated by using Qubit fluorometer with Quant-iT RNA BR assay kit. Libraries were prepared using Illumina TruSeq Stranded mRNA Sample Prep Kit + limited cycles of PCR by KAPA HiFi Polymerase following the directions of the manufacturer. RNA libraries were prepared for sequencing using standard Illumina protocols.