GSM4852255: Liver AIN-93M diet-fed WSB strain rep2; Mus musculus; RNA-Seq
Liver were removed, flash frozen on dry ice, and Total RNA was extracted from frozen liver samples with the Maxwell 16 LEV simplyRNA Tissue Kit according to the manufacturer's protocol. The RNA-seq libraries were constructed from total RNA following the Illumina TruSeq RNA library construction protocol. The size of the adapted fragments in the libraries was determined by running an Agilent DNA 1000 Chip. RNA libraries were prepared for sequencing using standard Illumina protocols