RNA-Seq of Arabidopsis branch joint : Columbia
Total RNA was extracted from about 50 mg Arabidopsis branch joints of 45-day-old seedlings. 1)Oligo dT Selection to enrich the mRNA; 2)RNA fragmentation and cDNA synthesis; 3)End repair, add A and adaptor ligation; 4)PCR; 5)Circularization and make DNB; 6) Sequencing on DNBSEQ platform.