GSM5067591: CLIP-seq_19L098; Homo sapiens; RIP-Seq
hTrmt13-RNA  complexes were isolated with antibody. The RNA is dephosphorylated and then removed modifications by Alkbs, an adaptor is ligated to the 3' end of the RNA and 5' end is radioactively labled. SDS-PAGE and transfer to nitrocellilose membrane anable to proremove free RNA and to stringently purify the crosslinked protein-RNA complexes. The RNA is then reverse trancribed in cDNA. Size selection of cDNA removes free primers, and is followed by cDNA circularization. Restriction enzyme digestion linearizes the cDNA before PCR amplification