GSM6217511: tumor02_ACT_C010-LK3-B; Mus musculus; OTHER
Cell permeabilization of native cells and tagmentation reaction with pA-Tn5 transposome-antibody complex for "Antibody-guided chromatin tagmentation (ACT-seq)" according to Carter et al (PMID: 31431618) and with a Tn5 transposome for "Assay for transposase-accessible chromatin by sequencing (ATAC-seq)" according to Corces et al. (PMID: 28846090). Tagmented DNA was either purified with MinElute (Qiagen) columns (ACTseq) according to the provider's instruction or with HighPrep beads (Biozym) after guanidinium-mediated inactivation of the transposome according to Weichenhan et al. (PMID: 29224141). Libraries were prepared according to Weichenhan et al. PMID: 29224141). Briefly, tagmented DNA was amplified by PCR under real-time conditions using a universal Illumina forward and a barcoded reverse primer and the NEBNext High Fidelity 2x PCR Master-Mix (NEB), and amplfication was monitored by inspecting real time the amplification curves. DNA was HighPrep bead-purified, Qubit (Invitrogen) quantitated and sized by TapeStation (Agilent) profiling.