PacBio Sequel HiFi of Hyles euphorbiae : Adult, male, Library 67064
Head tissue (38 mg) of Hyles euphorbiae was used for high molecular weight DNA extraction using an adaptation of the protocol by Miller et al. (1988). Final DNA purity and concentrations were measured using NanoPhotometer (Implen GmbH, Munich, Germany) and Qubit Fluorometer (Thermo Fisher Scientific, Waltham, MA). One SMRTbell library was constructed following the instructions of the SMRTbell Express Prep kit v2.0 with Low DNA Input Protocol (Pacific Biosciences, Menlo Park, CA). The total input DNA for the library was 3 g. The libraries were loaded at an on-plate concentration of 80 pM using diffusion loading. One SMRT cell sequencing run was performed on the Sequel System II in CCS mode using 30-hour movie time with 2 hours pre-extension and sequencing chemistry V2.0.