GSM4851795: Hut78-H3K27m3; Homo sapiens; ChIP-Seq
DNA was purified using QiAquick PCR Purification Kit (28106) after immunoprecipitation Ten nanograms of immunoprecipitated DNA was fragmented to 300 base pairs using a Covaris M220 Focused-ultrasonicator (Covaris, Inc., Woburn, MA) and then used to generate sequencing libraries using the Kapa HyperPrep Kit (Roche Sequencing and Life Science, Wilmington, MA). The size and quality of the library was evaluated using the Agilent BioAnalyzer, and the library was quantitated with the Kapa Library Quantification Kit. Each enriched DNA library was then sequenced on an Illumina NextSeq 500 sequencer to generate 50 million 75-base paired-end reads