GSM5727286: ny_2; Homo sapiens; RNA-Seq
Total RNA from PC3 and PC3-DR cells with three parallel samples from each group was extracted using TRIzol (Invitrogen, Carlsbad, CA, USA). The purity, concentration, and integrity of the total RNA were evaluated using an Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). RNA libraries were prepared for sequencing using standard Illumina protocols.