GSM5994206: B73, mrna-4 rep1; Zea mays; RNA-Seq
Two replicates were prepared per tissue. RNA was extracted from 5–10 individual plants per replicate. Equal amounts of the three replicates were used for sequencing. Total RNA was isolated from the 26 tissues using a Direct-zol RNA Microprep kit (Zymo Research) following the manufacturer's guidelines. RNA libraries were prepared for sequencing using corresponding standard protocols. mRNA-Seq, circular RNA-Seq, small RNA-Seq, ribo-Seq and PacBio Sequel