GSM5677864: CHB_03; Homo sapiens; miRNA-Seq
The serum was separated by centrifugation at 4000R / min for 5 minutes at 4 ° C.Then centrifuged at 1500 g for 10 minutes, and 15000 g for 30 minutes. Every 5 samples in the same group were mixed into a pool, with 5 pools in each group.Exoquick-5A™ (system Biosciences, Inc., Palo Alto, CA, USA) exosome Precipitation Kit was used to enrich EVs according to the manufacturer's instructions. Total RNA was extracted from serum EVs with mirneasy Mini Kit (Qiagen #217004 total RNA) (Qiagen, Valencia, CA). After qualified RNA  quality, the library was established and sequenced. Every 5 samples of the same group are randomly mixed into a pool. EVs RNAs were extracted from 5 pooled CHB serum samples, 5 pooled DeCi serum samples and 5 pooled healthy serum samples.