GSM6198811: H3F3A-specific siRNA, replicate 1; Homo sapiens; RNA-Seq
Total RNA extraction with Trizol. RNA purity and integrity were assessed with Nanodrop and agarose gel electrophoresis. mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. The mRNA was first fragmented randomly by addition of fragmentation buffer. The first strand of cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase (RNase H-). RNA libraries were prepared for sequencing using NEBNext Ultra RNA Library Prep Kit for Illumina