GSM5288616: KO1_LUNG; Mus musculus; RNA-Seq
LPS-induced leucocytes (> 95% PMNs) were collected from air pouch  and used for RNA extraction. Lungs were perfused, removed, flash frozen in liquid nitrogen, and RNA was harvested using RNeasy Mini Kit (QIAGEN) following the protocol. NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA)  was used with 1 ug of total RNA for the construction of sequencing libraries.