GSM5745477: BxPC3 cells, Erastin+Vitamin C 1; Homo sapiens; RNA-Seq
Cells were washed with cold PBS twice, and total RNA was isolated using RNAiso Plus (Takara) following the manufacturer's protocol. After verifying its concentration and integrity, the qualified RNA samples were subjected to PCR amplification to construct a cDNA library. The cluster generation and sequencing were performed on Novaseq 6000 S4 platform, using NovaSeq 6000 S4 Reagent kit V1.5. RNA libraries were prepared for sequencing using standard Illumina protocols