RNAseq on M. melolontha larval midguts
Larvae were offered for three consecutive days to feed on carrot sileces painted with i) water, ii) common dandelion latex or iii) common dandelion latex extract enriched in TA-G. Subsequently, larvae were dipped in liquid nitrogen and their anterior and posterior midguts removed by dissection. The gut tissue cleaned from the content was used for RNA extraction using innuPREP RNA Mini Kit (Analytik Jena, Jena, Germany). TrueSeq compatible libraries were prepared and PolyA enrichment performed before sequencing the transcriptomes.