Experiment2_HIV-1_LAI_WT_Method_B_Untreated_16h
Total RNA was isolated from THP-1 cells using a commercial kit called Total RNA Isolation System (Macherey-Nagel). RNA samples were quantified on a spectrophotometer (NanoDrop ND-1000, Thermo Fisher Scientific) and quality-analyzed in a Qubit fluorometer (Thermo Fisher Scientific). The sequencing libraries were generated using NEBNext Ultra II RNA Library Prep Kit (NEB) according to a protocol supplied by the manufacturer. Libraries were subjected to sequencing on a Hi-Seq 2500 platform at Genewiz, which generates 150 bp paired-end reads with a median depth of 4.7 million reads.