GSM5335382: Placenta Slc6a4-KO rep2 [S4]; Mus musculus; RNA-Seq
Placenta samples were collected, flash frozen in liquid nitrogen, and RNA was isolated using Qiagen AllPrep DNA/RNA/miRNA Universal Kit (Catalogue #80224; Qiagen, Germantown, MD). Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 500 ng of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols