GSM6340423: Lis38_early_naive_rep1_small; Homo sapiens; miRNA-Seq
RNA was extracted using mirVana miRNA Isolation Kit (Life Technologies, Inc.), following the manufacturers' instructions. RNA quality was assessed using a BioAnalyzer 2100 (Agilent Technologies, Inc.). All samples had a RIN greater than 8.5. Small RNA-seq libraries were constructed using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (New England Biolabs, Inc., Ipswich, MA). The libraries were pooled, size-selected for products that were 120-135 nucleotides in length using a Pippin Prep with a 3% agarose gel cassette (Sage Science, Beverly, Massachusetts), and run on a MiSeq instrument (Illumina, San Diego, California) at the UC San Diego UCSD Institute for Genomic Medicine (IGM) Genomics Core. Samples that produced adequate numbers of miRNA read counts were then rebalanced to produce similar numbers of miRNA reads and sequenced on a HiSeq 4000 instrument using 1x75 bp reads (Illumina, San Diego, California) at the UC San Diego IGM Genomics Core.