genomic row read
Qualified genomic DNA was fragmented with G-tubes (Covaris) and end-repaired to prepare SMRTbell DNA template libraries (with fragment size of >10 Kb selected by bluepippin system) according to the manufacturers specification (PacBio, Menlo Park, CA). Liraray quality was detected by Qubit and Average fragment size was estimated on a Bioanalyzer 2100 (Agilent, Santa Clara, CA). SMRT sequencing was performed on the Pacific Biosciences RSII sequencer (PacBio, Menlo Park, CA) according to standard protocols (MagBead Standard Seq v2 loading, 1  180 min movie) using the P4-C2 chemistry.