GSM5848735: WT luminal, BM1, RNA-seq; Mus musculus; RNA-Seq
RNA was isolated using the NucleoSpin kit (Macherey Nagel) 30ng of input RNA from each sample was barcoded during reverse transcription and pooled. Following Agencourt AMPure XP beads cleanup (Beckman Coulter), the pooled samples underwent second strand synthesis and were linearly amplified by T7 in vitro transcription. The resulting RNA was fragmented and converted into a sequencing-ready library by tagging the samples with Illumina sequences during ligation, RT and PCR. MARS-seq