GSM5213630: dopaminergic neurons from Brain 7-rep2; Homo sapiens; RNA-Seq
Tissue depleted of DA neurons after LMD was also collected from the slides in 100 µL of lysis/denaturing buffer from the RNAqueous micro kit (Life technologies, Zug, Switzerland). RNAs were extracted following the manufacturer protocol, quantified with a Qbit™ fluorometer (Thermo Fisher, Waltham, MA, US) and analysed with an Agilent 2100 Bioanalyser (Agilent Technologies, Palo Alto, CA) to check the RNA profile and obtain the RNA Integrity number (RIN). The SMART-Seq v4 kit from Clontech was used for the reverse transcription and cDNA amplification according to manufacturer's specifications, starting with 70 cells as input. 200 pg of cDNA were used for library preparation using the Nextera XT kit from Illumina.