GSM5745506: NJA5_IP_Cult_4; Mus musculus; RNA-Seq
RNA was extracted using the RNEasy Micro Plus kit (Qiagen 74034). For RNA sequencing RNA libraries were prepared with TrueSeq Stranded mRNA Library Prep Kit (Illumina), from on average 0.8ug total RNA. Poly(A) mRNA was isolated from total RNA samples, followed by mRNA fragmentation, first strand cDNA synthesis, second cDNA synthesis and adaptor ligation, and isolation and amplification of cDNA fragments. Illumina HiSeq 2500 instrument was used for sequencing, with a read length of 50bp single-end, and an average of 36 million reads per sample.