Selp low LT-HSCs (LSK SLAM) cells from aged C57BL/6
Bone marrow cells isolated from 28 month old female mouse, sorted for lineage-, Sca+, c-Kit+, Cd48-,Cd150+, Selp-low. RNAwas extracted with RNeasy Plus Micro Kit (QIAGEN), cDNA prepared with SMART Ultra-low input kit v4 (Takara), library prepared with Nextera XT Library Prep kit (Illumina)