RNA-seq FL 36h rep1
The protoplast cells from the out integument (OI) of ovules at different stages (-2 DPA to 2 DPA) were harvested using the PTED method. RNA extraction was performed in following our previous method (Wang et al., 2019a). The total RNA was reverse transcribed by using EasyScript One-step gDNA Removal and cDNA Synthesis SuperMix (AE311-03, Transgen Biotech, China) with oligo-dT and random primers. The DNBSEQ (BGI, China) with PE150 mode was used for sequencing the libraries.