GSM6219624: K562, RBFOX2 IP, MjDim1 treated, SAC-seq; Homo sapiens; RNA-Seq
RNA was extracted using TRIzol reagent (ThermoFisher). Ribosome RNA was depleted by RiboMinus™ Eukaryote Kit (ThermoFisher). RBFOX2-bound RNA was enriched by protein pulldown, and RNA was extracted with TRIzol reagent (ThermoFisher). Libraries were prepared by adaptor ligation and PCR.