16S rRNA paired-end reads (2 x 300 bp) (V3-V4 region) from human oral microbiome
Amplification of V3-V4 region of 16S rRNA gene according to the Illumina MiSeq's protocol. Amplification with KAPA HiFi HotStart ReadyMix PCR kit. Amplicons mixed with Illumina sequencing adaptors and dual-index barcodes (Nextera XT index kit v2, FC-131-2001).