GSM4982406: PBMCs_*03:01/*13:01_6; Homo sapiens; RNA-Seq
Buffy coats were processed by density gradient centrifugation using Ficoll (GE Healthcare Bio-Sciences AB, Uppsala, Sweden) and PBMCs were subsequently recovered. CD4+, CD8+, and CD14+ cells were isolated from the PBMCs via positive selection using CD4, CD8 or CD14 Microbeads (Miltenyi Biotec Norden AB, Lund, Sweden) on the autoMACS Pro Separator (Miltenyi Biotec Norden AB). Total RNA was extracted with the RNeasy Mini kit (Qiagen AB, Sollentuna, Sweden) according to manufacturer's instructions. The RNA was fragmented and prepared into sequencing libraries using the Illumina TruSeq stranded total RNA sample preparation kit with ribosomal depletion using RiboZero (2 x 125 bp) and sequenced on an Illumina HiSeq 2500 sequencer (SNP&SEQ Technology Platform, Uppsala, Sweden).