GSM5384655: KO5; Mus musculus; RNA-Seq
Total RNA from each heart was extracted by using RNAqueous™-Micro Total RNA Isolation Kit (Ambion, AM1931) following manufacturer's instructions. Transcriptome analysis of total RNA was performed by Novogene (https://en.novogene.com/). Only RNA samples with RNA integrity number (RIN) higher than 8.5 were selected for libraries preparation. 200 ng of total RNA was subsequently used to prepare RNA-Seq library by using TruSeq RNA sample prep kit (Illumina) according to manufacturer's instructions. Paired-end (2x150) RNA sequencing was performed on Illumina NovaSeq 6000 plataform (Illumina).