GSM5572863: PANC-1, negative control siRNA 1; Homo sapiens; RNA-Seq
Total RNA was isolated from cells using a ReliaPrep RNA Cell Miniprep System (Promega, Madison, WI) Total RNA were quantified and qualified by Qubit RNA Assay and TapeStation RNA ScreenTape. cDNA libraries for RNA sequencing were established from total RNA 500 ng using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs) for selecting poly-A mRNA followed by strand-specific library preparation using MGIEasy RNA Directional Library Prep Set V2.0 (MGI tech). The adapter sequences used in the library preparation are Forward: AAGTCGGAGGCCAAGCGGTCTTAGGAAGACAA; Reverse: AAGTCGGATCGTAGCCATGTCGTTCTGTGAGCCAAGGAGTTG The resulting double-stranded library fragments were further processed into single-stranded circular DNA (sscDNA), which is the final form of MGI library. The sscDNA libraries were quantified by Qubit ssDNA Assay Kit and used for generating DNA nanoballs (DNBs) by rolling circle replication reaction.