GSM5502315: Ctrl_2; Homo sapiens; RNA-Seq
Total RNA was isolated using the miRNeasy Mini kit (Qiagen), including the optional on-column DNA digestion with the RNase-Free DNase Set (Qiagen). After isolation, total RNA was used to generate polyA-enriched strand-specific cDNA libraries (TruSeq stranded mRNA library preparation kit, Illumina). Libraries were sequenced on a Illumina NextSeq 500 sequencer with HighOutput