RNA-seq of Arachis hypogaea : AMF inoculated and NaCl treatment replicate 3
Four-week-old seedlings with uniform sizes in both AMF inoculated and non-inoculated treatments were then selected. Then, half of the AMF inoculated and non-inoculated seedlings were exposed to salinity stress. Each pot was well watered with 200-mL of 200 mM NaCl solution for 3 times with a 2-day interval, while the rest of the seedlings were watered with distilled water at the same time. Finally, the average soil salt content was 2,400 mg kg-1 in salt treated pots and 600 mg kg-1 in non-treated pots. Totally, four treatments were composed: control (AMF non-inoculated + distilled water), AMF (AMF inoculated + distilled water), NaCl (AMF non-inoculated + NaCl solution), and AMF + NaCl (AMF inoculated + NaCl solution). The physiological parameters were determined at 12 d after the onset of salinity stress treatment.
L6