GSM4861193: gene expression in turnover; Mus musculus; RNA-Seq
Thymi were prepared by gently smashing and passing through a 40uM filter to prepare single cell suspensions. Thymocytes were sorted and sequenced using the Chromium Single Cell 5′ Library and the Gel Bead Kit (10x Genomics). Libraries were constructed using the Chromium Single-Cell 5′ reagent kit, V(D)J Mouse T Cell Enrichment Kit, 5′ Library Construction Kit, and i7 Multiplex Kit (10x Genomics, CA)