Illumina HiSeq 2500 sequencing; AD patients plasma exosome miRNA sequencing
AD patients plasma exosome miRNA sequencing
Sample 5_s
Human plasma exosomes were isolated via gradient centrifugation RNAs were isolated from exosomes using Qiagen RNeasy mini kit according to the manufacturers instructions.  Sequencing libraries were generated using NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB). The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq SR Cluster Kit v3-cBot-HS (Illumia).