E. coli cells transformed with the DfrB1 DMS library were incubated with different concentrations of inducer (0.01%, 0.025%, 0.05%, 0.2%, 0.4% arabinose), both with 10 g/mL trimethoprim and without trimethoprim, for 10 generations, with an intermediate dilution step at t = 5 generations. Sequenced at t = 0, t = 5, and t = 10 generations. 2 biological replicates for each combination of concentrations of arabinose and trimethoprim, with 2 technical replicates for each biological replicate.
E. coli cultures transformed with the DfrB1 DMS library were incubated with different concentrations of inducer (0.01%, 0.025%, 0.05%, 0.2%, 0.4% arabinose) for 10 generations. Sequenced at t= 0, t = 5, and t= 10 generations. 2 biological replicates for each concentration, with 2 technical replicates for each biological replicate
DfrB1-NovaSeq