GSM6006332: MB66RR; Mus musculus; miRNA-Seq
GSM6006332
The retinae were washed in RNAlater (Thermo Fisher Scientific) for 5 min., frozen in liquid nitrogen, and stored at -80°C. To isolate RNA, tissue samples were homogenized at 4°C in a lysis buffer using Bead Ruptor 24 tissue homogenizer (Omni). Small RNA fractions were extracted from each tissue sample using miRNAeasy mini kit (QIAGEN) following the manufacturer's protocol. Illumina sequencing libraries were constructed from 50 ng of small RNA using the TruSeq Small RNA Library Prep Kit (Illumina)