Illumina NovaSeq 6000 paired end sequencing; Osteogenesis-Related Long Noncoding RNA GAS5 as a Novel Biomarker for Osteonecrosis of Femoral Head
Osteogenesis-Related Long Noncoding RNA GAS5 as a Novel Biomarker for Osteonecrosis of Femoral Head
Sample 3_p
Finally, subchondral bone samples from six patients who underwent primary total hip arthroplasty (three ONFH patients and three patients in control group with femoral neck fracture) were obtained. All the samples were snap-frozen and stored in liquid nitrogen until further analyses. Frozen subchondral bone samples were rapidly ground in liquid nitrogen. Total RNA was extracted by TRIzol Reagent (Invitrogen) following the manufacturer's instructions. NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs), RiboZero Magnetic Gold Kit (Illumina), and KAPA Stranded RNA-Seq Library Prep Kit (Illumina) were used to achieve RNA enrichment and sequencing libraries generation.