GSM4983958: RNA-seq_D1_M_sham_rep2; Mus musculus; RNA-Seq
RNA was extracted from the FACS-sorted microglia using Qiagen RNeasy Plus Micro kit (Qiagen, 74034) RNA-Seq libraries were constructed using Nextera XT DNA Library Preparation Kit (Illumina, FC-131-1096) RNA was extracted from the FACS-sorted microglia using Qiagen RNeasy Plus Micro kit (Qiagen, 74034) in a final volume of 15μl. For construction of RNA-Seq libraries, total RNA performed first strand cDNA synthesis using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech, 634889) and second strand cDNA synthesis using KAPA HiFi HotStart DNA Polymerase (KK2502). After purification with beads (Agencourt AMPure XP, Beckman coulter, A63881) and libraries validation (Agilent 2100 bioanalyzer), RNA-Seq libraries were constructed using Nextera XT DNA Library Preparation Kit (Illumina, FC-131-1096).