ddrad seq of Pedicularis dudleyi
Libraries were constructed following a modified version of the protocol described in Peterson et al. (2012) where amplification was performed prior to size selection. 500  150ng of genomic DNA were digested using the restriction enzymes EcoRI and SphI-HF. P1 and P2 "flex" adapters were ligated to the digested DNA. Ligation products with similar concentrations were combined into a total of eight pools with each pool containing 10  24 samples. Each pool was then divided, and PCR amplified in eight separate 25 ul reactions using dual indexed PCR primers as described in Meyer and Kircher (2010). After amplification, indexed libraries were then re-pooled, quantified using qPCR and combined in equimolar ratios. The final library was size selected for 750bp and the final Illumina library was sequenced by Novogene at the UC Davis Sequencing Center on a single lane of Illumina HiSeq X Ten (150 bp, paired end)
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