GSM5069270: Ent_rep3; Ictidomys tridecemlineatus; OTHER
frozen liver tissue was homogenized by dounce in 10 mM Tris pH 7.5, 2 mM MgCl2, 3 mM CaCl2, 2 U/ml Superase-In, as described in Fang et al., 2014, Cell 159:1140 nascent RNA was labeled with BrdU in a 7 min nuclear run-on incubation at 30oC, purified by immunoprecipitation and converted to sequencing libraries as described in Fang et al., 2014 Cell 159:1140