GSM6304241: RNA_Pcgf24KO_EpiLC_rep3; Mus musculus; RNA-Seq
GSM6304241
Total RNA was extracted using the Qiagen RNeasy RNA kit with DNaseI on-column treatment.Prior to starting library preparation, RNA integrity quality was confirmed by RNA ScreenTape with TapeStation (RIN > 9).  Total 1 μg RNA was used for library preparation. RNA-seq library preparation was performed using the NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490) and NEBNext Ultra RNA Library Prep Kit for Illumina (E7770), according to the manufacturer's protocol. Illumina NextSeq 500, 75-bp single end