GSM4860005: WT RNA; Drosophila melanogaster; RNA-Seq
Cells were harvested and resuspended in lysis buffer (10mM Tris HCl pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.5% NP-40). Samples were treated with RQ1 DNase and RNA was extracted using TRIzol reagent. Illumina sequencing libraries were prepared using the PrepX RNA-seq protocol on the automated Apollo 324TM NGS Library Prep System (TakaraBio, CA).