GSM5218894: H9-dCas9-VP64-gRNA (HML-2) Day 10 tech rep 2; Homo sapiens; RNA-Seq
Total RNA from hPSCs as well as cortical neurons, at different time points, was isolated using TRIzoL (#15596-026, Invitrogen) following manufacturer's protocol. RNA extraction was performed using chloroform. RNA was precipitated in isopropanol and resuspended in nuclease free ddH2O. For RNAseq the RNA integrity number (RIN) was measured using the Agilent 2100 Bioanalyzer system.  RNA with a RIN value higher then 8 were sent for whole genome RNA sequencing. The TruSeq RNA Sample Preparation kit (Illumina) was used synthesizing the cDNA libraries and sequenced in paired-end-reads with the HiSeq4000 sequencer (Illumina). RNA libraries were prepared for sequencing using standard Illumina protocols