GSM4861206: WT-R1; Danio rerio; RNA-Seq
For each RNA sample, three eyes (lens removed) from different zebrafish were homogenized in RNAiso Plus reagent (Takara, Cat# 9108). Total RNA was extracted following the operation manual. The total RNA samples were quantified and qualified by Agilent 2100 Bioanalyzer (Agilent Technologies) and NanoDrop (Thermo Fisher Scientific). RNA samples with RIN ≥9.5 and A260/280 ≥1.9 were used for RNA-seq. The preparations of next generation sequencing library were constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina according to the manufacturer's protocol.