GSM5848806: Acute Tubular Necrosis - 538; Homo sapiens; RNA-Seq
GSM5848806
single nuclei were isolated from human kidney core needle biopsies under low lysis buffer and rotated using a stirrod for a total of 20 min. Nuclei were then washed by centrifugation and buffer replacement and subjected to 70um and 40um filtering. Libraries prepared under standard 10x and illumina guidelines. Libraries were generated using the 10x 5' Gene Expression kit single nuclei RNA-seq through 10x genomics platform