GSM4859383: HEK293_siBAHD1_FLAG_WTnum2_cell_PE_CR2_R1; Homo sapiens; OTHER
Cells were trypsinized with 0.05% trypsin and pellets were collected for CUT&RUN. WT-BAHD1-expressing cells labeled as WTnum1 and WTnum2 were processed in parallel with cells labeled as mutnum1 (W667G) and mutnum2 (Y669A), respectively. CUT&RUN was performed according to manufacturer's instructions (CUTANA™ pAG-MNase for ChIC/CUT&RUN, EpiCypher, Catnum 15-1116) with the antibodies against FLAG (Sigma catnumF1804). 10 ng of the purified CUT&RUN-enriched DNA was used for preparation of multiplexed sequencing libraries with the NEB Ultra II DNA Library Prep Kit per manufacturer's instructions (NEB numE7103).