GSM6199414: CD34_VHLKO_ATAC_rep1; Homo sapiens; ATAC-seq
GSM6199414
ATAC-seq was performed using the Omni-ATAC protocol. For each sample, 5-6 × 104 cells were lysed with buffer containing 0.1% NP-40, 0.1% Tween-20, and 0.01% digitonin, then the lysate was washed and incubated with Tagment DNA TDE1 Enzyme (Illumina) for 30 min at 37°C. DNA was purified with a Qiagen MinElute PCR Purification Kit. Library fragments were amplified using NEBNext® High-Fidelity 2X PCR Master Mix (NEB, M0541) and custom primers (table S4) with unique dual indexes. PCR amplification cycles were in accordance with the manufacturer's instructions. PCR products were purified using AMPure XP beads (Beckman Coulter, A63881) at a 1:1 ratio according to the manufacturer's instructions. Purified PCR products sized from 150 bp to 1 kb were purified from E-Gel EX Agarose Gels, 2% (Thermo Fisher, G401002). Libraries were pooled and sequenced in paired-end mode by using an Illumina HiSeq kit.