GSM5145764: 13nt_S1; Homo sapiens; ATAC-seq
60,000 HUDEP-2 cells were collected by centrifugation at 600 xg for 10 min at 4 °C. Cells were washed once with cold 1x PBS and centrifuged as above. Cells were lysed by gently pipetting to resuspend cell pellet in cold lysis buffer (10 mM Tris–HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630) and centrifuged as above. Cells were resuspended using the Illumina Tagment DNA TDE1 Enzyme and Buffer Kits (Illumina cat# 20034197) and incubated for 30 min at 37 °C. . Following purification, library fragments were amplified using NEBnext PCR master mix and custom Nextera PCR primers 1 and 2. Library amplification was determined using qPCR for a total of 13-15 cycles and then purified using a QIAGEN PCR purification kit. Library fragments were amplified using NEBnext PCR master mix and 1.25 μM of custom Nextera PCR primers 1 and 2 using the following PCR conditions: 72 °C for 5 min; 98 °C for 30 s; and thermocycling at 98 °C for 10 s, 63 °C for 30 s, and 72 °C for 1 min.  Libraries were amplified for a total of 17–19 cycles and then purified using AMPure XP beads.