RNAseq P. peruviana:leaves
High-throughput sequencing was performed on total RNA extracted using the Trizol method from 100 mg of leaf tissue  (Chomczynski and Sacchi, 1987). cDNA libraries were synthesized with the TruSeq Stranded Total RNA LT Sample kit (Illumina, USA); ribosomal RNA (rRNA) was removed with the Ribo-Zero Plant kit (Illumina). Sequencing was performed with the Illumina NovaSeq system.
BPP15