GSM4983790: T198; Homo sapiens; RNA-Seq
Tumor smple was fragmented and digested with collagenase IV (Gibco) and DNase I (Sigma). Cell fragments and red blood cells in the single cell suspension were removed with Human lymphocyte separation fluid (CL5020; Cedarlane).  And single cells were suspended in PBS containing 1% BSA. Cells were loaded on a Chromium Controller instrument. RNA libraries were prepared for sequencing with standard Illumina NovaSeq 6000