GSM6202227: KO time 0_2; Mus musculus; RNA-Seq
GSM6202227
RNA was isolated using the RNeasy Plus Mini Kit (Qiagen). RNA was used as input for cDNA synthesis using the Clontech SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio) according to the manufacturer's instructions. Amplified cDNA was fragmented and appended with dual-indexed bar codes using the NexteraXT DNA Library Preparation kit (Illumina). Libraries were validated by capillary electrophoresis on a Fragment Analyzer (Agilent)