GSM5219047: MDA231_Naive_rep3; Homo sapiens; RNA-Seq
The gels collected at the end of treatment and were mechanicaly and enzymatically disintegrated.  Next, the RNA was extracted from the tumor cells using RNA extraction kit (Qiagen), The lysates from in vitro samples were also supplemented with ERCC RNA spike-in mix to adjust for the cell number. RNA was sequenced using NovaSeq 150PE RNA libraries were prepared for sequencing using standard Illumina protocols