GSM6046546: rnaseq-FA272AsnSer-3; Homo sapiens; RNA-Seq
GSM6046546
RNA libraries were prepared for sequencing using standard Illumina protocols RNA was isolated using the RNeasy mini kit (QIAgen, Hilden, Gemany). For ChIPseq cells were crosslinked with 1% formaldehyde directly added to tissue culture media and lysed in 5mM PIPES pH 8.0, 85 mM KCL and 0.5% NP40 and cell nuclei recovered. Nuclei were lysed in 1% SDS, 10 mM EDTA pH 8.0 and  50 mM Tris-HCL, pH 8.0. Nuclei were sonicated to a DNA average size of 300bp. IP was performed with Sirt6 antibody from Abcam (Cambridge UK) ab191385