GSM5386934: ryp1 RT B [SB1405D]; Coccidioides posadasii str. Silveira; RNA-Seq
RNAs were isolated using a modified acid-phenol prep as described previously (Mandel et al 2006 PMID: 16857399) with the modification that liquid N2 grinding was used to initially disrupt the cells. RNAs were resuspended in diethyl pyrocarbonate-treated dH2O prior to assessment of their quality and concentration with an Agilent Bioanalyzer (Agilent Technologies, Palo Alto, CA). RNA-seq libraries were prepared as previously described (Rodriguez et al 2019, PMID 31568523). Briefly, for the isolation of mRNAs, five μg total RNA from each sample was subjected to poly-A purification using Dynabeads Oligo (dT)25(Invitrogen-ThermoFisher). RNA libraries were prepared using NEBNext Ultra Directional RNA Library Prep Kit (NEB). Quality of the total RNA, mRNA and library was confirmed using Bioanalyzer (Agilent).