GSM6202423: wt thymus xray rep 1 [paired-end]; Mus musculus; RNA-Seq
GSM6202423
Expression profiling by high throughput sequencing of spleen, liver and thymus in wt p53 mice and mice with a deletion of the C-terminus of p53. Organ pieces are immediately placed in RNAlater during dissection and stored at 4oC until RNA is extracted using RNeasy Mini Kit (Qiagen) and following manufacturer's instructions.  The isolated RNA is quantitated and either stored at -80o in preparation for RNA-seq. Sequencing libraries prepared with the TruSeq Stranded Total RNA kit (Illumina Inc), from 1ug total RNA