GSM5503298: PHx3; Mus musculus; RNA-Seq
Liver sinusoidal endothelial cells were isolated from sham and PHx mice, total RNA was isolated from the cells using the Trizol (invitrogen). The purified library products were evaluated using the Agilent 2200 TapeStation and Qubit®2.0 (Life Technologies, USA) and then diluted to 10pM for cluster generation in situ on the HiSeq3000 pair-end flow cell followed by sequencing (2×150bp) on HiSeq 3000. RNA libraries were prepared for sequencing using standard Illumina protocols.