GSM6008266: tumor pericytes, TCF21-overexpressing, TCF21 Input; Homo sapiens; ChIP-Seq
GSM6008266
The tumor pericytes was fixed in 1% formaldehyde for 10 min at room temperature , after which 0.125 M glycine was added and the mixture was sat for 5 min to terminate the crosslinking reaction. The tumor pericytes was then collected and frozen in liquid nitrogen, it was treated with cell lysis buffer and nucleus was collected by centrifuging at 2000g for 5min. Then, nucleus was treated with nucleus lysis buffer and sonicated to fragment chromatin DNA The high-throughput DNA sequencing libraries were prepared by using VAHTS Universal DNA Library Prep Kit for Illumina V3（Catalog NO. ND607, Vazyme）. The library products corresponding to 200-500 bps were enriched, quantified and finally sequenced on Novaseq 6000 sequencer (Illumina) with PE150 model.