GSM5219080: Derived from CD34+ progenitors from CB; Homo sapiens; RNA-Seq
Cells were loaded in the ChromiumTM Controller for partitioning single cells into nanoliter-scale Gel Bead-In-Emulsions (GEMs) aiming at a recovery of 12000 cells. Single Cell 3′ reagent kit v3.1 was used for reverse transcription, cDNA amplification and library construction of gene expression libraries (10x Genomics) according to the manufacturer's instructions. TotalSeqTM-A libraries were prepared following the protocol for 10x Single Cell 3′ Reagent Kit v3.1 provided by BioLegend, including primer sequences and reagent specifications. All libraries were quantified using a QubitTM 2.0 Fluorometer (ThermoFisher) and quality was tested on a 2100 Bioanalyzer with High Sensitivity DNA kit (Agilent). Libraries were sequenced on a NovaSeq 6000 S1 Flow cell (CeGaT GmbH Tübingen), read configurations were set to 28 (R1), 9 (i7), 91 (R2). Antibody and hashtag libraries were amplified using the KAPA HiFi ready mix with primers containing Illumina Small RNA RPIx sequences and Truseq D70x_s, respectively. Chromium Next GEM Single Cell 3ʹ Reagent Kit v3.1 