Pool-Sequencing of Dallia pectoralis females
Pool-seq libraries were generated using the Truseq nano DNA sample prep kit (Illumina, ref. FC-121-4001) following the manufacturers instructions. Briefly, each pool was sonicated using a Bioruptor (Diagenode). The sonicated pools were repaired, size selected on magnetic beads aiming for a 550 pb insert size and adenylated on their 3 ends. Adenylated DNA was ligated to Illuminas specific adapters and, after purificaiotn on magnetic beads, was amplified in an 8 cycles PCR. Libraries were purified using magnetic beads, checked on a Fragment Analyzer (Agilent) using the HS NGS Fragment kit (DNF-474-33) and quantified by qPCR using the KAPA Library quantification kit (Roche, ref. KK4824). Each libraries was sequenced on half a lane of a rapid v2 flow cell (Illumina) in paired end 2*250nt mode
Dalpec_female_poolsex