GSM5218447: Day 20; Mus musculus; RNA-Seq
Tumor-bearing hemisphere were harvested, percoll gradient isolation of immune cells were conducted, CD45hi/loCD11b+ cells were sorted by FACS analysis, and RNA was harvested. Chromium Single Cell 3'V3.1 Reagent Kits (10X Genomics, Pleasanton, CA) was used for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols