GSM5067989: NPC-6; Homo sapiens; RNA-Seq
Eight samples per condition (all technical replicates from one experiment) were fixated in QIAzol (Qiagen) and stored at -80 °C until further processing. Whole RNA extraction was performed following the manufacturer's protocol using the RNeasy® mini kit (Qiagen) with a DNase digestion step (Qiagen). RNA concentration was determined using the Qubit3 (Invitrogen) and RNA quality was analyzed using the 2100 Bioanalyzer (Agilent Technologies). Samples were processed according to the TruSeq Stranded mRNA protocol (Illumina, San Diego, CA, USA). Enrichment of mRNA was done using polyA-affinity purification converting it into a multiplex library of 16 samples.