GSM4859391: HEK293_siBAHD1_WT_cell_H3K27me3_ChIP; Homo sapiens; ChIP-Seq
Lysates were clarified from sonicated nuclei and protein-DNA complexes isolated with specific antibodies against H3K9me3 (Abcam cat# ab8898), H3K27me3 (Abcam cat# ab6002), Anti-acetyl-Histone H3 (Lys9) Antibody (Millipore cat# 06-942) and H3K27ac (Abcam cat# ab4729). Libraries were prepared using the NEB Ultra II DNA Library Prep Kit per manufacturer's instructions (NEB #E7103).