GSM5070528: RNAseq-WT-testis-4dpp; Mus musculus; RNA-Seq
Total RNA from frozen testis samples and from sorted germ cells was extracted using mirVana miRNA isolation kit (Thermo Fisher, AM1560). Library construction for strand-specific RNA libraries was performed as previously described (Zhang, Z., Theurkauf, W.E., Weng, Z., and Zamore, P.D. (2012). Strand-specific libraries for high throughput RNA sequencing (RNA-Seq) prepared without poly(A) selection. Silence 3, 9.)