GSM5150353: Adipocyte_ko_1151; Mus musculus; RNA-Seq
Adipocytes were isolated from subcutaneous adipose tissue via collagenase digestion, and RNA was extracted using the Rneasy Lipid Tissue Minikit. Livers were homogenized in TRIzol, and RNA was chloroform extracted. RNA quality was assessed via BioAnalyzer (RIN: Adipose (>6), Liver (>9)). Poly-A pull-down was used to enrich mRNAs from total RNA samples, and RNA libraries were prepared using the Illumina TruSeq RNA prep kit for sequencing using Illumina NextSeq 500 at Columbia Genome Center.