GSM5835529: CHiC HH18 posterior trunks; Gallus gallus; Hi-C
GSM5835529
Micro-dissected E9.5 posterior trunks and HH18 posterior trunks were isolated in PBS supplemented with 10% Fetal Calf Serum and dissociated into single cell by collagenase treatment (10 µl of collagenase 50mg/ml (Sigma C9697) at 37º for approximately 15 mn). Samples were cross-linked with 2% formaldehyde (ThermoFisher 28908) for 10 minutes at room temperature and washed three times with PBS containing proteinase inhibitor. After discarding the supernatant cells were stored at -80º. Further processing was performed as described in (Yakushiji-Kaminatsui et al., 2018) Following the Hi-C protocol, ligated DNA fragments were processed through the Agilent SureSelectXT protocol to enrich for fragments coming from the probe regions chr2:72240000-76840000 (mm9) and chr7:14946000–17870000 (galGal5).