GSM5502542: ANI treated sample rep 2; Homo sapiens; RNA-Seq
Total RNA was purified with the GeneMATRIX universal RNA purification kit (EURx), including a DNase (Promega) on-column digestion step. 350 ng of total RNA as quantified by Qubit (Invitrogen) was used for subsequent RNA-Seq. Ribosomal RNA was depleted with the Ribo Zero Human Gold Kit (Illumina) Libraries were prepared using the TruSeq Directional RNA LT Preparation Kit (Illumina) using standard Illumina protocols.  Before library preparation RNA was prepared without poly(A)-mRNA enrichment step for acquiring pre-mRNA