PacBio sequencing of BS71
BS71 DNA was prepared for sequencing by inoculating a single colony onto a 10% Tryptic Soy agar plate, scraping the biomass generated, and extracting the DNA using the Qiagen genomic DNA protocol (Qiagen, Valencia, CA). The DNA was not sheared or size selected. Whole genome sequencing was completed at the University of Massachusetts Medical School (UMMS) sequencing center. A PacBio SMRTbell library was constructed and sequenced on the PacBio RS II platform, generating 150,292 raw reads and 69,059  filtered reads.
BS71_filtered_subreads.fastq