Whole metagenomics sequencing of Homo sapiens: stool samples of travelers to Cusco, Peru
Metagenomic DNA was extracted from stool samples using phenol-chloroform repeated bead-beating protocol. Extracted metagenomic DNA was diluted to 0.5 ng/uL and prepared for sequencing using a Nextera DNA library protocol. Sequencing libraries were submitted for 2x151 bp paired-end sequencing on an Illumina NextSeq High-Output platform. Before submitting the sequencing data, adapter and indexes were trimmed and sequenced were quality filtered using trimmomatic, and human sequences were removed using deconseq.
P246S02