GSM5746500: D1_DC; Homo sapiens; RNA-Seq
Human dendritic cell cultures were frozen and stored at -80°C before shipping. Library preparation was performed with the Illumina TruSeq Stranded mRNA (incl. poly-T oligo pulldown) technology, according to the manufacturer ́s protocol. Then, samples were quality controlled using the RNA 6000 Pico LabChip kit was used on the 2100 Bioanalyzer (Agilent Technologies). All libraries were quantified using the highly sensitive fluorescent dye-based Qubit® ds DNA HS Assay Kit (Thermo Fisher Scientific). All single libraries were pooled into several sequencing library pools with an equal DNA amount per sample. Subsequently, the sequencing library pools were pooled separately with libraries of other projects for sequencing in several NextSeq HighOutput runs. Clustering of the library pools was performed on the NextSeq® 500 sequencing system (Illumina). RNA libraries were prepared for sequencing using standard Illumina protocols