GSM5929665: Exp35-HB-22F1; Mus musculus; RNA-Seq
GSM5929665
Total RNA from healthy brain was extracted by using the Promega-Maxwell 16 LEV Simply RNA Tissue kit, following the manufacturers' indications. Total RNA from sorted GBM cells was extracted by using Rneasy Micro Kit (Qiagen), following the manufacturers' indications. RNA library preparations and sequencing reactions were conducted at GENEWIZ, LLC. (South Plainfield, NJ, USA). SMART-Seq v4 Ultra Low Input Kit for Sequencing was used for full-length cDNA synthesis and amplification ,  and  Illumina  Nextera  XT  library  was  used  for sequencing library preparation. Briefly, cDNA was fragmented, and adaptor was added using Transposase, followed by limited-cycle PCR to enrich and add index to the cDNA fragments.