GSM5848799: Fibrotic kidney allograft rep 2; Homo sapiens; RNA-Seq
GSM5848799
Single nuclei were isolated using Nuclei EZ lysis buffer with protease inhibitor. The homgenate was filtered with a 40um cell strainer and, then, centrifuged at low speed. The nuclei pellet was resuspended in Nuclei resuspspension buffer and passed through the 40um filter. Libraries were prepared according to standard 10x Genomics and Illumina instructions. Libraries were generated using the 10x 5' Gene Expression kit. Single nuclei RNA-seq (10x genomics platform)