GSM5746553: 4_WWA-22; Mus musculus; ATAC-seq
Tissues were prepared as described by 10X Genomics Demonstrated Protocol – Nuclei Isolation from Mouse Brain Tissue for Single Cell ATAC Sequencing Rev B with some modifications. Isolated nuclei were then used as input following the 10X Genomics Chromium Next GEM Single Cell ATAC Reagent Kits v1.1 manual. Targeting a 5,000 nuclei recovery, samples were added to the tagmentation reaction, loaded into the Chromium Controller for nuclei barcoding, and prepared for library construction following manufacturer's protocol (10X Genomics PN-1000175).