GSM4859315: salmonella-day13-single lane; Mus musculus; RNA-Seq
Colons were enzymatically dissociated and digested with collagenase/dispase to single cell suspensions. Live CD4+ TCRb+ cells were sorted using the MoFlo Astrios. Single cells were then encapsulated in nanodroplets according to the 10x Genomics protocol. 10X Genomics library prep (3' V1, V2 or 5' V1) Library were sequenced using a NextSeq500.