CTL2
Cells were treated by Emetine dihydrochloride hydrate to prevent mRNA decay before mRNA extraction. mRNA was then extracted from primary fibroblasts with TRIzol and RNeasy Plus Mini kit (Qiagen). RNA was then reverse-transcribed with SuperScript III reverse transcriptase. The full-length cDNA for STAT3 was amplified by PCR with specific oligonucleotide primers. After fragmentation and library prep, sequence was performed using MiSeq nano sequencing (Illumina) to detect the rare splicing events.
CTL2