GSM5068094: Ly6Chi monocytes 3d injury 1; Mus musculus; RNA-Seq
Total RNA was extracted from fresh frozen cell pellets using Qiagen RNeasy Plus Universal mini kit following manufacturer's instructions Libraries  prepared using the NEBNext Ultra RNA Library Prep Kit for Illumin.  mRNAs were first enriched with Oligo(dT) beads. Enriched mRNAs were fragmented for 15 minutes at 94 °C. First strand and second strand cDNAs were subsequently synthesized. cDNA fragments were end repaired and adenylated at 3'ends, and universal adapters were ligated to cDNA fragments, followed by index addition and library enrichment by limited-cycle PCR.