Haliclona oculata metagenome sequence reads
For DNA extraction bead beating was performed with the DNAeasy PowerLyser Powersoil Kit at 4000 rpm for 45 s, followed by an overnight incubation at 56 C  with 0.2 M EDTA, 10 % CTAB, 0.5 M NaCl,  0.1 M EDTA (1:4 ratio), _-Mercaptoethanol, and Proteinase  K. RNAse A (0.3 ng/_l) was then added and the sample incubated for 30 min at 37 C. Chloroform/Isoamylalcohol (24:1) addition, incubation (2 min), and washing (centrifugation at 10000 x g for 10 min) was performed twice. DNA was precipitated in isopropanol for 6 hours, pelleted, and washed twice in ethanol before DNA elution in water. The Nextera DNA Library Prep Kit (Illumina) was used to prepare the sequence library with 25 ng of extracted DNA.
O_S4.2