GSM5572838: Total RNAseq of cell sample c16; Homo sapiens; RNA-Seq
Total RNA was isolated from untreated and treated CCD-1070Sk using RNeasy Mini Kit (QIAGEN) according to manufacturer's instructions. Total RNA was quantified by the Qubit 2.0 Fluorometer (Life Technologies) and run on the Bioanalyzer 2100 (Agilent) to analyze the RNA integrity. RNA (1-5μg) with RIN 7-10 was used for ribosomal RNA depletion using the Ribo-Zero kit (Illumina) according to the manufacturer's instructions. Strand specific library construction and sequencing on the 5500 × l SOLiD™ System. For sequencing on the Illumina sequencers, library preparation was done using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). The libraries were sequenced at Genome Quebec, Montreal, Quebec and sequenced on the Illumina HiSeq4000 (PE100) and at the Illumina Epigenetics Platform, University of Lethbridge, Lethbridge, Alberta on the NextSeq 500 system.