Illumina NovaSeq 6000 sequencing; RNA sequencing of WT and Zeb1200M islets
RNA sequencing of WT and Zeb1200M islets
WT 1_s
Islets were isolated from mice by perfusing Liberase enzyme into the common bile duct. RNA was extracted using the Picopure RNA purification kit, including on-column DNase treatment.  Library construction was performed using Illumina Truseq mRNA kit.