GSM6355822: PANC-1 siCtrl-3; Homo sapiens; RNA-Seq
GSM6355822
NA was extracted from sorted ROR1high and ROR1low cells using MagMAX-96 Total RNA Isolation Kit (Thermo Fisher Scientific), and 1 ng of RNA was used for the reverse transcription reaction using SMART-seq HT (Takara).  For PANC-1 cells, RNA was harvested using ReliaPrep RNA Cell Miniprep System (Promega). RNA-seq library was prepared using Nextera XT Library Prep Kit (Illumina). For PANC-1 cells, RNA libraries for RNA-seq were prepared using NEBNext Ultra RNA Library Prep Kit fir Illumina (NEB) following manufacturer's protocols.