GSM5149486: WT NPC unsorted rep 1; Homo sapiens; OTHER
Genomic DNA was isolated by phenol:Chloroform:isoamyl alcohol and purified by ethanol precipitation Two rounds of PCR was done with isolated genomic DNA. Barcoded primers were used in PCR reactions to produce CRISPR amplicon library for each sample. PCR amplicon libraries were sequenced on Illumina Nextseq 500 sequencer.