GSM5411874: MeCP2 KO rep 4; Mus musculus; RNA-Seq
Total RNA was isolated from dissected tissue using the RNeasy Mini Kit (Qiagen, 74104) A double-stranded DNA library was created using 250ng of total RNA. First, cDNA was created using the fragmented 3' poly (A) selected portion of total RNA and random primers. Libraries were created from the cDNA by first blunt ending the fragments, attaching an adenosine to the 3' end and finally ligating unique adapters to the ends. The ligated products were then amplified using 15 cycles of PCR.