RNA-seq of Triticum aestivum cultivar Basalt; SAM tissue; 13 days after end of vernalization
The SAM and leaves materials were collected at three Waddington stages (W): W1.25-W1.75 (transition apex phase TAP), W2.0-W2.5 (double ridge stage DRS) and W3.0-W5.0 (late reproductive phase LRP), which correspond to time points 5, 13 and 25 days after the end of vernalization (DAV). Depending on the development stages of each cultivar at the time of collection, the pooling of 20 to 60 shoot apices was needed to reach the minimum weight of tissue required for RNA extraction. We strictly selected shoot apices that showed a uniform morphological development per time point. The distal part of leaves samples was harvested at the same time points as mentioned above and from the same plants from which SAM was collected. For each cultivar, three biological replicates were collected. The samples were frozen immediately in liquid nitrogen and stored at -80C. Total RNA extraction from the collected tissues, initial quality control and sequencing analysis were performed commercially at Novogene Co. Ltd. (HK, China). Considering two cultivars*two tissues* three time points*three biological replications, 36 libraries were constructed and sequencing based on the sequencing platform NovaSeq 6000 (Illumina) using the sequencing strategy paired-end 150 (=PE150) yielded on average 52.76 million 100 bp paired-end reads per sample.
B-TP2-SAM-REP1