GSM5218346: 6W_ATP5MD; Homo sapiens; OTHER
4C assay libraries were generated according to previously established protocols (van de Werken, H. J. G. et al. 4C technology: protocols and data analysis. Methods in enzymology 513, (Elsevier Inc., 2012).). In brief, the chromatin was cross-linked with formaldehyde, followed by two rounds of degestion-ligation using two different 4-bp cutters, MboI and NlaIII. The quality of 4C products was assessed on agarose gels. 4C templates were then amplified with specific 4C-primers, which were designed for correponding bait regions. Primers pairs were pooled according to primer efficiency and PCR products were purified. Thereafter, 4C products with primers contained sequencing adaptor sequences.