GSM5746937: 5xFAD rep1; Mus musculus; RNA-Seq
Pulmonary B cells of WT and 5xFAD mice were obtained using MACS. The cells were flash frozen on dry ice, and RNA was harvested using Trizol reagent. RNA libraries were constructed by using rRNA-depleted RNAs with TruSeq Stranded Total RNA Library Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer's instructions. Libraries were controlled for quality and quantified using the BioAnalyzer 2100 system (Agilent Technologies, Inc., USA).