GSM5068632: 266_siArid1a_rep3; Mus musculus; RNA-Seq
Total RNA was harvested by TRIzol method from triplicate (siCtrl samples are in duplicates) samples and quality was assessed by running 500 ng RNA on an agarose gel. Libraries were prepared from 500 ng RNA utilizing Truseq RNA Library Prep Kit v2 following manufacturer's instructions. mRNA was captured by RNA purification beads and eluted. The first strand and second strand cDNA were synthesized and the overhangs of the fragments were repaired into blunt ends. The 3' end was added with a single adenine to prevent mutual ligation during the following adapter conjugation. Finally, the DNA fragments with both flow-cell-hybridization adapters were amplified and the library was quantified by Agilent Bioanalyzer 2100. Size selection was optional if the main population of the library exceeds the appropriate size for sequencing or small fragments such as primer-dimers exist.