GSM5502299: P-limited T. erythraeum under ambient condition - rep1; Trichodesmium erythraeum IMS101; RNA-Seq
Total RNA was extracted using TRIzol Reagent. Genomic DNA was removed thoroughly by treating with RNAase-free DNase I. Ribosomal RNA was removed from a total amount of 3 µg RNA using Ribo-zero kit (Illumina). Sequencing libraries were generated using NEBNext Ultra Directional RNA Library Prep Kit. Sequencing libraries were generated using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer's recommendations.