RNA-Seq of Homo sapiens: adult saliva
Samples with positive qPCR result underwent RNA extraction using the QIAamp Viral RNA (Qiagen) kit for the QIAcube according to the manufacturers instructions. RNA extracts were then treated using the Kapa Hyper Prep with RiboErase according to the manufacturers instructions. Library quantification was conducted by KAPA Library Quant Kit (Illumina) Universal qPCR Mix Kit, the libraries were normalized to 4 nM, and pooled for sequencing on the Illumina NextSeq 500/550 High Output Kit v2.5 (300 Cycles, 150  150 bp). FASTQ data was processed through UltraSEQ as described above.
15953_080421_U_S9