GSM6359049: C2201; Lithobates clamitans; RNA-Seq
GSM6359049
RNA was extracted using the RNeasy Plus Mini Kit from QIAGEN. At step 3 of the protocol, we used 350µL of buffer RLT and a TissueLyser II (QIAGEN) set to 30 Hz for one minute for disruption and homogenization. RNA was stored at -20 Celsius before processing. mRNA was isolated, cDNA libraries constructed, and unique sample indexes added using the TruSeq RNA Library Prep Kit (Illumina) following the manufacturer's protocol.