GSM5502023: DZ_10129_late [DJ-7]; Homo sapiens; OTHER
Genomic DNA was isolated in digestion buffer (100 mM Tris-HCl, pH 8.5, 5 mM EDTA, 0.2% SDS, 200 mM NaCl), Proteinase K (Thermo Fisher cat# EO0491) treatment at 55 °C for overnight. The second day, phenol:chloroform:isoamyl alcohol (25:24:1 Saturated with 10 mM Tris, pH 8.0, 1 mM EDTA) (Sigma-Aldrich, cat# P-3803) was added to samples, mixed completely, and centrifuged for 10 min at 12,000 rpm. The aqueous layer solution was transferred into a new Eppen- dorf tube and precipitated with 600 mL isopropanol. The pellet was washed with 75% ethanol, air-dried, and eluted with Nuclease-Free Water (Ambion). hMe-Seal assay (NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina + single end 50bp Hiseq 2000 Illumina sequencing)