Illumina HiSeq 4000 paired end sequencing; RNA-seq of human midbrain organoid models of Parkinson's Disease
RNA-seq of human midbrain organoid models of Parkinson's Disease
Sample 4_p
Total RNA was isolated from hMLOs using TRIzol Reagent (Thermo Fisher). DNA contaminants were digested with DNASE I (Ambion). For RNA-seq library preparation, total RNA was further purified using the Purelink RNA Mini kit (Ambion). 1.5 ug total RNA was used for library construction according to manufacturer's protocol (TruSeq stranded mRNA Library Preparation Kit, Illumina).