WT-mRNA-seq
Hemocytes were manually dissected from 3rd instar larvae in batches of 50, stored at -80C until hemocytes from 5000 animals had been collected and then pooled. mRNA was isolated using a MACS mRNA Isolation Kit (Miltenyi Biotec Inc) and mRNA-seq libraries prepared using a SMARTer Stranded Total RNA-Seq Kit v2 - Pico (Takara).
WT-mRNA-seq