RNA-seq of felis catus: adult and juvneile testicular tissue
Total RNA was isolated from up to 10 mg of tissue using PureLinkTM RNA Mini Kit with on-column DNase Set. Only samples with RIN 7 were used for library preparation. Sequencing libraries were generated using TruSeq Stranded mRNA LT Sample Prep Kit from Illumina. Fragments were selected with insert sizes between 200-400 bp. The libraries were sequenced 150 bp paired-end with 30 million reads depth per sample.
AS-Seq13