Permafrost soil sample, Chile, 5260 m, 10 cm depth, 16S rRNA amplicon seq
V3V4 region of the 16S rRNA gene was amplified with fusion primers containing Bacteria-specific sequences Bakt_341F (5-CCT ACG GGN GGC WGC AG-3) and modified Bakt_805R (5-GAC TAC NVG GGT ATC TAA TCC-3) (Herlemann et al. 2011) with Fluidigm CS1 and CS2 universal sequences at their 5' ends.
A52.10.2