GSM5067773: endometrial epithelial organoids derived from an unidentified healthy donor 3; Homo sapiens; RNA-Seq
organoid cells were pelleted and resuspended in 1 ml trizol reagent (invitrogen) and stored at -80. thereafter, tubes were thawed on ice, vigourously vortexed (~2 min) to homogenize before the addition of 200 ul chloroform per tube. tubes were vigourously vortexed and contents passed through 5prime heavy-gel phase-lock tubes (andwin scientific) by centrifugation (14,000 x g for 7 min at 4 degrees c). the supernatant was processed according to the direct-zol rna extraction protocol (zymo research) according to manufacturer instructions. Libraries were prepared using an Illumina TruSeq mRNA kit (Illumina Inc.).