GSM5146054: Pdibot_Microglia_rep1; Mus musculus; RNA-Seq
whole brains except olfactory bulb and cerebelum were removed from p17 mice. Different glia cell types were enriched using immunopanning method. CD45 was used to purify microglia. GalC hybrydoma was used to purify Differentiated oligodendrocytes. O4 hybrodoma was used to purify oligodendrocyte precursor cells. Hepacam was used to purify astrocytes. Total RNAs were collected using Qiagen miRNeasy kit. cDNAs were generated using the Nugen Ovation V2 kit (Nugen), then fragmented using the Covaris sonicator. Sequencing libraries were prepared using the Next Ultra RNA Library Prep kit (New England Biolabs) with 12 cycles of PCR amplification.