RNAseq of mouse hippocampus
Mice were decapitated at the end of the study, the hippocampus carefully dissected, immediately snap frozen in liquid nitrogen and stored at -80C until use. For transcriptomics the right hemisphere of the hippocampus was used and total RNA extracted using the RNeasy Plus Universal Mini Kit (Qiagen, Hilden, Germany; Cat. no. 73404) according to the manufacturer's instructions. For tissue dissociation, 2.0ml SC Micro Tube PCR-PT (Sarstedt, Nmbrecht, Germany; Cat. no. 72.694.406) were filled with 800mg  100mg Zirconia/Silica beads (BioSpec Products, Inc., Bartlesville, OK, US; Cat no. 11079101z, 0.1 mm diameter). RNA concentration and quality were determined using a Nanodrop 1000 (Thermo Scientific), followed by Bioanalyzer (Agilent) to measure RNA integrity (RIN). Samples with a RIN > than 8.5 were considered for sequencing. Library preparation and sequencing as well as Fastq-file generation was done by Edinburgh Genomics (Edinburgh, United Kingdom) using paired end sequencing on an Illumina NovaSeq 6000.
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