WGS of Bacillus pumilus
Samples were collected from the floor of the Spacecraft Assembly Facility at the Jet Propulsion Laboratory between February 2 and July 28, 2016. Samples were collected from 1 m2 of the floor using a 23x23 cm premoistened polyester wipe, placed in a glass bottle containing phosphate buffer saline, thoroughly mixed for 30 s, and concentrated using 0.45 m contracting pipette tip (InnovaPrep, Drexel, MO, USA). An aliquot of 425 L was heat shocked (80C, 15 min), and grown on tryptic soy agar incubated at 32C. DNA was extracted using the ZymoBIOMICS DNA MagBead kit with provided instructions. Whole genome sequence (WGS) libraries were prepared with the Illumina Nextera DNA Flex kit and sequenced with a NovaSeq 6000 S4 flow cell (paired-end 2x150-bp). Sequences were quality controlled using the FastQC (v.0.11.7) and fastp (v.0.20.0) to remove poor quality and adapter sequences. The WGS was assembled using SPAdes (v.3.11.1), and QUAST (v.5.0.2) was used to determine the genome size, number of contigs, and N50 value. Default settings were used for all programs, excluding fastp (which used 512 adapters for screening). Species identity was determined using OrthoANIu, with a minimum average nucleotide identity of 95%.
179-K 3C2 HS