ddRADseq and epiRADseq data from Kenyan house sparrows
Sequencing was performed on an Ion Torrent Personal Genome Machine. For library creation, we modified a standard GBS protocol for ddRAD and epiRAD sequencing on the Ion Torrent platform. For ddRAD, we used enzymes MspI and PstI. For epiRAD, we used enzymes HpaII and PstI. HpaII and MspI cut DNA at the same sequence (CCGG), but HpaII is sensitive to methylation at the restriction site, allowing us to calculate population genetic statistics and compare the presence or absence of methylation among individuals.
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