GSM6008202: Ribo_Conidia_H153G_DMSO1; Colletotrichum orbiculare; OTHER
GSM6008202
Cells were filtered, scraped, and soaked in liquid nitrogen with 600 µl of lysis buffer. The frozen cells and lysis buffer grains were milled by a Multi-beads Shocker at 2,800 rpm for 15 se for 1 cycle. RNase I treatment, ribosome pelleting, size selection, preadenylated linker ligation, rRNA depeletion, reverse transcription, cDNA circularization, and PCR amplification.