GSM6202344: D4-500-3_Rep3; Mus musculus; RNA-Seq
GSM6202344
Crosslinked and TF-Sorted RNA-seq was performed as described in the associated manuscript. In brief, cells were PFA fixed, stained with intracellular antibodies and flow sorted. After reverse crosslinking, RNA was extracted using the RecoverAll RNA/DNA Isolation Kit (Thermo Fisher Scientific AM1975) Libraries were built using SMART-Seq HT kit (Takara Cat no 634437) followed by Nextera XT DNA Library Preparation Kit (Illumina FC-131-1096). Prior to library preparation 1 ul of ERCC spike-ins was added for QC later assessments