Illumina NovaSeq 6000 paired end sequencing; RNA-Seq of wild type and osmtd2 mutant rice anthers
RNA-Seq of wild type and osmtd2 mutant rice anthers
mtd2-3_p
The samples were collected from paddy field at anthesis stage. We froze the anthers immediately using liquid nitrogen.  Plants were transplanted to paddy field after two weeks in incubator at BOD incubator at end of May. The plants were then grown for 3 months in the field. We extract RNA from the sample using RNeasy Plant Mini Kit from QIAGEN following manufacturer's instruction.   TruSeq Stranded mRNA Sample Preparation Guide, Part #15031047 Rev. E with TruSeq Stranded mRNA LT Sample Prep Kit