GSM5746942: OVCAR-5 shCtrl rep 3; Homo sapiens; RNA-Seq
RNA was harvested using TRI Reagent (Sigma Aldrich). gDNA was removed using QIAGEN RNeasy MinElute Cleanup Kit. Then mRNA was isolated using NEBNext Poly(A) mRNA Magnetic Isolation Module. RNA libraries were prepared using NEBNext Ultra II RNA Library Prep kit for Illumina.