GSM5849441: plasma microRNA rep17; Homo sapiens; miRNA-Seq
GSM5849441
Total plasma RNA was extracted from the plasma of individuals using the miRNeasy Serum/Plasma Kit (Qiagen, Hilden, Germany) following the manufacturer isolation protocol. Illumina small RNA-Seq kits (Clontech, Mountain View, CA, USA) were then used to generate cDNA libraries. Sequencing of the plasma miRNomes was completed with the Illumina HiSeq 4000 platform (Illumina, San Diego, CA, USA), with single-end 50bp reads.