GSM5503784: FACS endothelial cells Young Bleo 20; Mus musculus; RNA-Seq
Lungs were perfused, harvested and processed for FACS. Freshly isolated lung endothelial cells were sorted diretly in tubes containing 350 ul of RLT buffer, for RNA isolation and subsequent RNA-seq analysis Takara Bio SMART-Seq v4 Ultra Low RNA kit to generate cDNA followed with Illumina Nextera lib prep