WGS of Burkholderia pyrrocinia strain Hargis genome by Pacific Biosciences Sequel
Genomic DNA was extracted using Qiagen DNEASY Microbial DNA Extraction; library preparation was done using a Pacific Biosciences SMRTbell Express Template prep kit v2.0, with DNA sheared to 7-10kb using Covaris gtubes. Each sample was barcoded with PacBio Barcoded adapters; libraries were pooled at equal nM and size selected to 7-10kb using SAGE Blue Pippin.
Hargis_PacBio