GSM5411602: Myocd-LSD1_H3K4me2-3; Rattus norvegicus; OTHER
Genomic DNA after immunoprecipitation was extracted  by CUT&Tag-IT Assay Kit (Active motif) following manufacturer's protocol. 500,000 cells were used for CUT&Tag assay. After binding with Concanavalin A beads, cells were incubated with primary antibody targeting H3K4me2 (Activemotif 39141) or FLAG (Sigma F7425) overnight at 4℃. Samples were then incubated with Guinea Pig anti-Rabbit antibody, CUT&Tag-IT Assembled pA-Tn5 Transposomes. After tagmentation, released DNA was extracted by DNA purification column for library preparation and SPRI Beads clean-up. DNA library was perpared by illumina Nextera primers provided  with CUT&Tag-IT Assay Kit.