GSM6304395: immature non-confined DCs, WT control, rep1; Mus musculus; RNA-Seq
GSM6304395
RNA was harvested using RNeasy mini plus kit (Qiagen). RNA sequencing libraries were prepared from 300ng to 1µg of total RNA. TruSeq Stranded mRNA Library preparation kit and the Illumina Stranded mRNA Prep Ligation kit which allow to perform a strand specific RNA sequencing. A first step of polyA selection using magnetic beads is done to focus sequencing on polyadenylated transcripts. After fragmentation, cDNA synthesis was performed and resulting fragments were used for dA-tailing and then ligated to the TruSeq indexed adapters (for the TruSeq kit) or RNA Index Anchors (for the mRNA Ligation kit). PCR amplification was finally achieved to create the final indexed cDNA libraries (with 13 cycles).