Seq_Trifolium Pratense L.
DNA extraction from 1-RCDY was carried out followed by PCR. The species identification was done by gene marker site using GeneAmp PCR System 9700. The amplified PCR product is subjected to electrophoresis on an agar medium (agarose gel) to confirm the amplification of the gene site. The base sequence was determined through an automatic sequencing equipment using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems TM). Species are identified by comparison and analysis with the nucleotide sequence registered on the NCBI Genebank.
1-RCDY