GSM6202291: EA2_InVivo_3; Mus musculus; RNA-Seq
GSM6202291
Total RNA was isolated using RNeasy mini plus kit (Qiagen), including the genomic DNA elimination step. Libraries were constructed using an Illumina TruSEQ stranded mRNA Sample Prep Kit (Cat# RS-122-2101). Total RNA was combined with RNA purification beads to bind PolyA RNA to oligodT magnetic beads. mRNA was eluted and converted to double stranded DNA. A-tailing, adapter ligation, and PCR amplification using 15 cycles was performed to complete the library construction.