Amplicon sequencing of peptone-enriched water samples
Metagenomic DNA was extracted from water samples which were enriched with diluted peptone. Subsequently, the V3-V4 region of 16S rRNA genes was amplified. All samples were amplified in triplicate, purified and pooled in equal amounts. Sequences were determined using an Illumina MiSeq sequencer.
DAIF_2018_WSW_Bac_DNA