Illumina NovaSeq 6000 paired end sequencing; Transcriptomic analysis of pediatric T-cell acute lymphoblastic leukemia
Transcriptomic analysis of pediatric T-cell acute lymphoblastic leukemia
2101_PA_P3_p
T-cells were obtained from bone marrow samples by isolation of mononuclear cells, using density gradient centrifugation, followed by immunomagnetic separation, with use of Human T Lymphocyte Enrichment Set-DM (Becton Dickinson) Total RNA was isolated using miRCURY RNA Isolation Kit Cell & Plant (Exiqon) or miRNeasy Kit (Qiagen). RNA isolates were DNase treated and purified with use of RNA Clean and Concentrator Kit (Zymo Research). Libraries were generated with Illumina TruSeq Stranded mRNA Prep Kit. Modified fragmentation conditions were used (90'C for 2 minutes)