GSM5746091: ATAC-Seq D2_Irf4_cKO_P14; Mus musculus; ATAC-seq
Cell nuclei were extracted and proceed directly to the Tn5 transposition reaction and library preparation. Briefly, Tn5 transposase (Illumina #FC-121-1030) was used for genomic DNA fragmentation and the transposed DNA fragments were PCR amplified and barcoded with Illumina Nextera Index Kit (FC-121-1011).