GSM5950042: ACC_young input_rep2; Mus musculus; RIP-Seq
GSM5950042
Brain regions were dissected on ice and flash frozen. RNA was extracted from tissue using TRI Reagent (Sigma) and cleaned with the clean and concentrator kit (Zymo research). RNA was processed as described previously (Berulava 2020): rRNA was depleted  (NEBNext ribosomal RNA removal kit, NEB) and fragmented to ~80 nt with fragmentation reagents (Invitrogen). 10 ug of rRNA depleted RNA were pooled from 3-4 mice for each replicate and used for immunoprecipitation with an m6A-speciic anitbody. Inputs correspond to 500 ng of RNA that were not immunoprecipitated, . Mouse samples were prepared with the TruSeq Stranded Total RNA Library Prep Kit (Illumina).