GSM5502610: WT single fiber 3; Mus musculus; ATAC-seq
EDL muscle was dissected and myofibers were isolated as previously described (Blackburn, D.M. et al. Journal of Biological Chemistry, 2019). After isolation and selection of a single myofiber, myofibers were lysed with water and then permeabilized with 0.5% Triton X-100  for 15 minutes at room temperature (RT). The permeabilization step was followed by two washes with PBS1X. 5000 MuSCS were freshly isolated by Fluorescence Activated Cell Sorting (FACS) as previously described from Pax7/GFP+ mice  (Tichy, E et al. Skeletal Muscle, 2018). Myofiber ATAC-Seq samples were lysed as described above and the ATAC-Seq library preparation was performed with modified version of OMNI ATAC-Seq protocol (Corces, M.R. et al. Nature Methods, 2017). Isolated MuSCs were freshly sorted into ATAC-Seq lysis buffer and samples were processed with modified version of the OMNI ATAC-Seq protcol (Corces, M.R. et al. Nature Methods, 2017).