GSM5218457: CD57- CD4+ TEM_rep 3; Homo sapiens; RNA-Seq
Different indicated T cell subsets are FACS-purified from PBMCs of three healthy donors with >95% purity. Cells are immediated submmited to GenElute Total RNA isolation kit (Sigma) for total RNA extraction according to the instruction, and the quality and integrity of RNA samples are validated by Bioanalyzer (Agilent) with RIN values > 8.0. The mRNA library is prepared by ACRF Biomolecular Resource Facility at ANU using Illumina TruSeq Stranded mRNA Library Prep kit and sequenced in one high-output flow cell with 75 bp single-end reads by NextSeq500 sequencer (Illumina), each sample was covered with at least 25 million reads.