GSM4983674: HSC WT, rep1; Mus musculus; RNA-Seq
HSCs (CD34-/LSK) (1*10e3 cells) were sorted from WT and CD74-/- mice (BD FACS Aria III). Sequencing libraries were prepared using the SMART-Seq v4 Ultra Low Input RNA kit (Clonetech). Sequencing libraries were constructed containing barcodes. Single end reads were sequenced on one lane of an Illumina HiSeq2500 machine.