GSM5746148: HiChIP_3T3_CTCF; Mus musculus; OTHER
Cells were fixed with 1% formaldehyde and lysed according to the published HiChIP protocol (Mumbach et al., Nature Methods 2016). Briefly, nuclei were isolated, digested with MboI restriction enzyme, filled-in with biotin-dATP, lysed with sonication, immunoprecipitated with indicated antibody and then the biotin labeled DNA was pulled-down with streptavdin beads. Libraries were constructed based on the published HiChIP protocol (Mumbach et al., Nature Methods 2016). Briefly, the pulled-down DNA was fragmented with Tn5 transposase, PCR amplified and barcoded with Illumina dual indexes. The amplified DNA fragments were size-selected (300-700 bp) wih AMPureXP beads. HiChIP