GSM5150521: Shell-Female-3; Rattus norvegicus; RNA-Seq
Adult rat's brain were rapidly removed, flash frozen using cold isopentane, stored at -80°C. Brain was sliced coronally at 200 µm thickness. Nucleus accumbens shell and core was punched. 50 mg frozen tissue of NAc shell or NAc core from 4 animals of the same sex were pooled. Tissues were homogenized and nuclei were obtained as reported by Nagy et al., 2020. Gel beads in emulsion was made by using 10X Genomics chromium controller and barcoding using chromium NEXT GEM single cell 3' reagent kits v3.1 and chromium Next GEM Chip G single cell kit. GEMs were incubated at 53°C to break emulsions, cDNA was then bulk amplified for 11 cycles and standard Illumina libraries were prepared.