GSM5503733: Tumor 1 PG1hi; Homo sapiens; RNA-Seq
Freshly isolated NCI-N87 tumors were enzymatically digested and the dissociated cells were sorted according to the fluorescence of PG1 and AF647. Total RNA isolation was performed with the Qiagen RNeasy micro kit. A cDNA library was prepared using a SMART-Seq v4 Ultra Low Input RNA kit for Sequencing (Takara Bio) and a Nextera XT Index Kit v2 SetA/B/C/D (Illumina).