RNA-Seq of Hediste diversicolor: adult head
Total RNA extractions from the head and the middle body (reciprocal distance from the head and the tail) were performed by NZY Total RNA Isolation kit (NZYTech, Lda. - Genes and Enzymes) following the manufacturers recommendations. Strand-specific libraries were built by Macrogen, Inc. These libraries included inserts between 250 and 300 bp, and were sequenced on the Illumina HiSeq4000 platform using 150 bp paired-end reads. The raw datasets (head and middle body) were inspected using the FastQC software (version 0.11.8), quality-filtered with Trimmomatic (version 0.38) software and corrected for random sequencing errors with Rcorrector (version 1.0.3).
Hdiv1