EVA71 in wastewater in Arizona, USA, 2020
The libraries were prepared using KAPA Hyperplus Library Kit (Roche) following the manufacturer's user guide. The concentration of cleaned DNA was evaluated using the Qubit dsDNA HS Assay Kit (Life Technologies). 500 ng DNA was used to prepare the libraries. The samples underwent fragmentation, end repair and A-tailing, adapter ligation and bead cleanup.  This was followed by a limited-cycle (8 cycles) PCR and bead cleanup. Following the library preparation, the final concentration of the libraries was measured using the Qubit dsDNA HS Assay Kit (Life Technologies), and the average library size was determined using the Agilent 2100 Bioanalyzer (Agilent Technologies).  The libraries were pooled,  diluted and sequenced paired end for 251 cycles using the MiSeq system (Illumina).
5-WW-05062020_S13_L001