GSM6202855: ChIP-seq_CNS-28_Naive_CD4_H3K27ac_rep1; Mus musculus; ChIP-Seq
GSM6202855
Detailed procedures for ChIP-Seq analysis was performed as described previously (Hu et al., 2018). For RNA-Seq, 2ng of total RNA were extracted miRNeasy Micro Kit (50) from Qiagen (Cat # 217084). ChIP DNA were end-repaired using an End-It DNA End-Repair kit (Epicentre), universal adaptors were then ligated. The libraries were amplified using indexed primers with the PCR condition: 98°C, 10”; 67°C, 30”; 72°C, 30” for 16 cycles. The fragments between 180bp to 350bp were isolated on E-gel and subjected to sequencing on Illumina HiSeq2500.