Modelling Multi-Dimensional ClinOmics for Precision Therapy of Children and Adolescent Young Adults with Relapsed and Refractory Cancer: Sample NCI0185.tumor.DNA
DNA was extracted from fresh frozen tumor tissue using AllPrep DNA/RNA kits (Qiangen, Germantown). One hundred nanograms of DNA was sheared to approximately 300 base pairs (bp) by sonication (Covaris, Woburn, MA). DNA library construction and enrichment was performed using SeqCap EZ Exome v3.0 Kits according to the manufacturer's instructions (Roche, Wilmington, MA) . Paired-end sequencing (2X101 bp) was performed on an Illumina HiSeq2000 instrument.
NCI0185tumor_E