RNA-Seq of Paratelphusa hydrodromous
The total RNA from the crab leg tissue samples were extracted by using the RNeasy Mini Kit (cat. nos. 74104 and 74106) with on-column DNAse treatment following the manufacturers instructions (Qiagen). 10 g of total RNA was used to prepare the RNA seq libraries using the TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego, CA). After the libraries were constructed, a paired-end run was performed on Illuminas HiSeq 2500 platform (Agri Genome Labs Pvt Ltd., Kochi, India) to generate 15-20 million paired-end reads per sample.
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