GSM5575071: db/db day 0 wound skin replicate 2; Mus musculus; RNA-Seq
Total RNA from skin and macrophages was isolated using Rneasy kit (Qiagen) rRNA removal was performed either by Ribominus Eukaryotic system v2 kit (ThermoFisher Scientific). rRNA depleted RNA was subjected to preparation of Illumina compatible NEBNext Ultra II Directional RNA Library Preparation kit (NEB).