GSM5453554: MDA231_H182_2uM_2; Homo sapiens; RNA-Seq
Total RNA was prepared using Qiagen RNeasy Mini kit according to the manufacturer's instructions. Library construction was performed using the Illumina TruSeq Stranded mRNA library preparation kit. Briefly, total RNA samples were assessed for concentration using a Qubit fluorometer and quality using the 2100 Bioanalyzer. Up to one µg of total RNA per sample was used for poly-A mRNA selection. cDNA was synthesized from enriched and fragmented RNA using reverse transcriptase and random primers. The cDNA was further converted into double-stranded DNA (dsDNA), and the resulting dsDNA was enriched with PCR for library preparation. The PCR-amplified library was purified using Agencourt AMPure XP beads. The concentration of the amplified library was measured with a Qubit fluorometer and an aliquot of the library was resolved on a Bioanalyzer.