ciliate protozoa 18S rRNA gene amplicon of bovine: rumen liquid
Rumen liquid samples were collected through fistula. Samples were aliquoted, snap frozen in dry ice and stored at -80 oC until DNA extraction. Total DNA was extracted as described by Rius et al. (2012).  For ciliate protozoa amplicon sequencing universal primers targeting 18S rRNA gene were used (Sylvester et al., 2004).
CAI6