GSM5849630: JY_13_IM_Control_PBS_1; Mus musculus; RNA-Seq
GSM5849630
PBS vs WGP-trained mice were euthanized on Day 7 following treatment to harvest the lungs. Lungs were digested and processed to obtain single cell suspensions. The Lung single cell suspensions were stained with fluorochrome-labelled antibodies to obtain the Lung Interstitial macrophages. Cells were then washed with Ice-cold PBS, stored in TRIzol followed by RNA extraction (QIAGEN RNAeasy Kit), quantification IQubit fluorometric assay) and sequencing. Libraries were prepared using the Universal Plus mRNA-Seq with NuQuant (NuGEN Cat# 0520-24) and PolyA selection.