GSM5503009: 6_16h_SKMEL28_SCR_Tram_3; Homo sapiens; RNA-Seq
RNA was extracted using the Qiagen RNeasy Mini Kit, following manufacturer's instructions except that lysates were loaded on a QIAshredder column before loading on a spin column. Sequencing libraries were generated from 100ng input RNA using the Illumina TruSeq Stranded mRNA LT Sample Preparation Kit as per manufacturer's instruction. RNA libraries were prepared for sequencing using standard Illumina protocols