GSM5503277: EP_H3K4me3_ChIPSeq Rep 1 (re-analysis); Mus musculus; ChIP-Seq
Nuclei were isolated from cells using hypotonic lysis. Nuclei were fixed with 1% formaldehyde, and chromatin was fragmented by Mnase digestion. Cross-links were reversed and chromatin eluted by incubation at 68 degrees Celsius. After high-speed spin soluble chromatin was removed and used for ChIP with anti-H3K4me3. Libraries were prepared for sequencing using standard Illumina protocols.