GSM5849654: Lung, tumor; Mus musculus; RNA-Seq
GSM5849654
1×10^6 luciferase-labeled LLC cells were subcutaneously injected on the dorsum of mice to construct spontaneous lung metastatic models. The murine tissues in this study were prepared from mice sacrificed by cervical dislocation at day 14 after tumor-bearing. For the isolation of lung epithelial cells, Lung tissues were collected, cut into small pieces and incubated in dissociation solution with 2 mg/ml Collagenase type I, 2 mg/ml Collagenase type IV and 25 mg/ml DNase (Sigma). The solution was pipetting every 10 minutes during the incubation and suspension was dispersed through a 70 mm cell strainer. To purify AT-II lung epithelial cells, single-cell suspensions from lungs were stained with antibodies, and CD45- SftpC+ cells were sorted using a MoFlo XDP flow cytometer (Beckman-Coulter) with purities of >95%, followed by 10× library preparation and sequencing. All the remaining procedures including the library construction were performed according to the standard manufacturer's protocol (CG000206 RevD).