GSM5849473: stimulated B cells, MKO, 2C; Mus musculus; RNA-Seq
GSM5849473
Naive B cells were purified from spleens of 12-week old mice by negative selection using anti-CD43 microbeads. Cells were then stimulated in serum-containing RPMI media with lipopolysaccharide and interleukin-4 for 48 hours. Cells were harvested and their total RNA extracted using Quick-RNA™ Microprep Kit (ZymoResearch). mRNA was isolated using the NEBNext® Poly(A) mRNA Magnetic Isolation (New England BioLabs). Stranded Illumina libraries were prepared with Swift Rapid RNA Library Kit according to the manufacturer's instructions (Swift Biosciences). Indexed libraries were sequenced on a HiSeq X Ten platform, and an average of 30 million 150-bp paired-end reads were generated for each sample (Novogene, Beijing, China).