GSM5069011: GA-M4_S9; Mus musculus; RNA-Seq
The apical region of the first mandibular molar was collected for RNA isolation with an RNeasy Micro Kit (QIAGEN). The quality of RNA samples was evaluated using an Agilent 2100 Bioanalyzer and all samples used for sequencing had RNA integrity (RIN) numbers >9.0. cDNA library preparation and sequencing were performed by DNA Link, Inc. Single-end reads with 75 cycles were performed on NextSeq500 for three pairs of samples.