GSM5068860: E35 skeletal muscle input DNA; Sus scrofa; ChIP-Seq
Samples were  were washed with PBS and incubated in lysis buffer. Chromatins were sonicated to get soluble sheared chromatin (average DNA length of 200–500 bp). The histone-DNA complexes were isolated with H3K27ac. Immunoprecipitated DNA was used to construct sequencing libraries following the protocol provided by the Illumina TruSeq ChIP Sample Prep Set A and sequenced on Illumina HiSeq X Ten with PE150 method