GSM5746318: DMSO-treated replicate2; Mus musculus; RNA-Seq
RNA was collected using Qiagen RNA extraction kit RNA quality was assessed on a Fragment Analyzer (Agilent Technologies). All RNAs had an RQN between 9.4 and 10. RNA-seq libraries were prepared from 500 ng of total RNA with the Illumina TruSeq Stranded mRNA reagents (Illumina) using a unique dual indexing strategy, and following the official protocol automated on a Sciclone liquid handling robot (PerkinElmer). Libraries were quantified by a fluorometric method (QubIT, Life Technologies) and their quality assessed on a Fragment Analyzer (Agilent Technologies).