GSM6355487: HEK293T, 0 CpGs, Rep1; Homo sapiens; synthetic HIV-1; RNA-Seq
GSM6355487
ZAP:RNA complexes were crossedlinked by exposure to UV radiation for 2min. Cells were lysed and ZAP was purified by immunoprecipitation. RNA bound to ZAP was released by proteinase K digestion and phenol:chloroform:isoamyl alcohol extraction. A fluorescently labelled 3' adapter was ligated to the RNA, gel purified and subsequently ligated to a 5'adapter. Resulting nucleic acids were reversed transcribed and amplified using Illumina primers.