GSM5693889: SUB235985, SUB235955, SUB235966, SUB235964, SUB235998 [s5a-cite]; Homo sapiens; RNA-Seq
Cells were stained as per the CITE-seq_and_Hashing_protocol_190213 protocol (found here: https://citeseq.files.wordpress.com/2019/02/cite-seq_and_hashing_protocol_190213.pdf).  Cells were pooled 4 or 5 samples to a pool and captured using the Chromium Single Cell 3' Reagent Kit v2 Revision F (found at https://support.10xgenomics.com/single-cell-gene-expression/index/doc/user-guide-chromium-single-cell-3-reagent-kits-user-guide-v2-chemistry) Libraries were constructed as per the manufacturer's protocol in the Chromium Single Cell 3' Kit v2, Revision F.  In brief, captured mRNAs were reverse transcribed prior to the breaking of cell/gel bead droplets.  cDNAs were recovered and underwent amplification.  SPRI beads were used to clean the libraries and split them into gene expression and antibody-dependent libraries.  The GEX libraries underwent end repair, polyA tailing, and fragmentation prior to further size selection to extract 300-500 bp fragments and the ligation of sequencing adaptors; the ADT libraries were likewise purified and had sequencing adaptors added.