GSM4859797: E13.5 Sox9+Scx+ attachment progenitors; Mus musculus; RNA-Seq
For collection of cells by FACS, 4 forelimbs from 2 E13.5 embryos were dissected and suspended in cold PBS. Single cell suspension were achieved by trypsin and collagenase type V (dissolved in DMEM, Sigma) digestion. Single-cell 3' RNA-seq libraries were generated according to the manufacturer's protocol (10x Genomics Chromium Single Cell 3' Reagent Kit User Guide v3/v3.1 Chemistry). The libraries were sequenced using NovaSeq 6000 instrument using an SP 100 cycles reagent kit   (Illumina) (R1 28 bases, R2 82 bases and I1 8 bases). Single-cell RNA sequencing (scRNA-Seq)