Illumina NovaSeq 6000 paired end sequencing; RNA-seq of developing seeds of Samgwang, a japonica rice cultivar, grown in normal or reclaimed lands
RNA-seq of developing seeds of Samgwang, a japonica rice cultivar, grown in normal or reclaimed lands
Sample 5_p
Developing rice (Oryza sativa) seeds of Samgwang were harvested from one panicle per plant (total five plants per a replication). Normal land indicates just common paddy field with very low salt concentration. Reclaimed land contains extremely high concentration of sodium, potassium, magnesium and sulfur, compared to normal land.  Total RNA was extracted from developing seeds of Samgwang using the RNeasy Plant Mini Kit (QIAGEN, Hilden, Germany), according to the manufacturerâ€™s instructions.  TruSeq RNA Sample Prep Kit v2 was used for library construction.