GSM5070434: APP1_miRNA; Mus musculus; miRNA-Seq
Three male APP/PS1 transgenic mice and three wild type littermates were used for RNA-seq. Total RNA (containing miRNA) was isolated from hippocampus with TRIzol reagent (Thermo Fisher Scientific, CA, USA), according to the manufacturer's instructions. Total RNA was quantified with Qubit 3.0 Spectrophotometer (Thermo Fisher Scientific, CA, USA). RNA purity was evaluated with NanoDrop 2000 (Thermo Fisher Scientific, CA, USA). RNA integrity was assessed with 1% agarose gels and Agilent 2100 Bioanalyzer (Agilent Technologies, CA, USA). The sequencing libraries for mRNA and circRNA were generated by TruSeq Stranded Total RNA Library Prep Kit (Illumina, CA, USA).The sequencing libraries for miRNAs were generated by TruSeq small RNA sample Preparation kit (Illumina, CA, USA). After cluster generation using TruSeq PE Cluster Kit v3-cBot-HS (Illumia, CA, USA), the libraries were sequenced on an Illumina Hiseq 2500 platform.