GSM6202406: A549, LRPPRC, rep1; Homo sapiens; RIP-Seq
GSM6202406
The cell lysates were incubated with a 2 μg anti-LRPPRC antibody (Abcam) or rabbit IgG control (Millipore). Then the cell lysates were incubated with protein A/G modified beads for 4 h at 4℃ in a rotator. co-immunoprecipitated RNAs were extracted by miRNeasy Mini Kit (QIAGEN, 217004) for RNA sequencing or PCR verification. A total amount of 250pg—10ng RNA per sample was used as input material for sequencing libraries using the SMARTer Stranded Total RNA-Seq Kit V2 (Takara Bio USA, Inc.) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.