Illumina HiSeq 2500 sequencing; Transient GFP-fused DARPin expression effects on transcription
Transient GFP-fused DARPin expression effects on transcription
Sample 2_s
Cells are collected by FACS (GFP-positive, except for WT group) RNA is extracted with RNeasy Micro Kit 125ng of total RNA was processed using the Illumina TruSeq Stranded mRNA kit (cat. 20020595), according to the manufacturer protocol, with 14 cycles of final PCR amplification.