GSM5849542: LHX6_KO_2; Mus musculus; RNA-Seq
GSM5849542
Cortical tissue was dissected in ice-cold Hanks' Balanced Salt Solution, digested with Accutase, dissociated, resuspended and filtered using cell strainers. YFP+ve cells were sorted using MoFlo XDP Cell Sorter directly into tubes prefilled with 300 µl Neurobasal medium. Total RNA was isolated from freshly sorted cells without freezing using RNeasy Plus Micro Kit (Qiagen, #74034) according to manufacturer's manual. RNA quality was assessed using TapeStation RNA ScreenTape (Agilent) according to manufacturer's manual. The NEBNext Single Cell/Low Input RNA Library Prep Kit was used to generate indexed amplified cDNAs libraries total RNA. The libraries were pooled sqeuenced on the NextSeq 500  (lllumina) using a single 75bp read configuration.