Illumina HiSeq 3000 paired end sequencing; RNA-seq of human embryonic stem cells H1 ESC cells and the differentiated NPC cells with FOXK1/2 and SIN3A knock down and the control condition.
RNA-seq of human embryonic stem cells H1 ESC cells and the differentiated NPC cells with FOXK1/2 and SIN3A knock down and the control condition.
H1ESC_siNTforSIN3A_rep3_p
Pluripotent H1 HESC cells were cultured in mTeSR1 (Stemcell Technologies) on Matrigel precoated culture surface, cells were passaged every 4-6 days with ReLeSR as clumps of 50-100 cells. Differentiation into NPC was performed for up to 5 days with STEMdiffâ„¢ Neural Induction Medium (Stemcell Technologies) at 37deg incubator with 5% CO2. RNA was collected using Qiagen RNeasy kit. RNA-seq libraries were constructed using Illumina TruSeq Stranded mRNA kit.