GSM5502471: BAT ZT17 ATAC 1 [CLH_ATAC_13]; Mus musculus; ATAC-seq
NuTRAP nuclei and ribosomal RNA were isolated according to established methods (PMID 28122230). Brown adipose and inguinal white adipose depots were snap frozen in liquid nitrogen, minced, dounce homogenized, spun down and FACS sorted to isolate for ATAC-Seq. Tissues were minced, homogenized, ribosomal RNA was affinity purified using anti-GFP antibodies and protein G Dynabeads and extracted using the Qiagen Micro RNeasy kit. ATAC-seq libraries were constructed according to the Omni-ATAC protocol (PMID 28846090). RNA-Seq libraries were constructed from 250 ng of RNA using the NEBNext RNA Ultra Directional library prep kit