NGS of base editing in C. glutamicum: GBE-Cg-R2
DNA fragments containing the target region (200-250 bp) were amplified from the extracted genomic DNAs by PCR. NGS libraries were constructed by using NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs, MA, USA) or VAHTS Universal DNA Library Prep Kit for Illumina (Vazyme Biotech, Nanjing, China). Library construction and sequencing were performed by GENEWIZ (Suzhou, China).
GBE-Cg-2