RNAseq of human PBMCs: male non-infected by  M. tuberculosis
Total RNA extraction was done from the PBMCs using the commercial kit RNeasy Plus Mini Kit (Qiagen).Samples with an RNA Integrity Number (RIN) 7, ratio of 260/280 1.8 and 28S/18S ratio 1.5 were selected for sequencing.The cDNA libraries generated paired-end reads, and were prepared using TruSeq stranded mRNA library kit (Illumina, California), and the sequencing was done by Macrogen Inc, (Seoul, Korea), using NovaSeq system (Illumina).
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