RNA-Seq of Medicago truncatula leaf
The fifth trifoliate leaf was harvested in a 1.5 mL microcentrifuge tube containing two 3.2 mm diameter steel beads (Lab Supply Ltd) and homogenized to a fine powder using the Qiagen TissueLyser II. RNA samples for RNAseq were extracted using the Plant RNA purification reagent (InvitrogenTM Thermo Fisher Scientific) following the manufacturers instructions. Six libraries were prepared using NEXTflex Rapid Direc-tional qRNA-SeqTM Kit (Bio Scientific) as instructed by the manufacturers protocol. The same amount of each RNAseq library was pooled and run on one lane of a 125PE HiSeq2000 (Illumina).
NZGL01919AC-23