RNA-Seq of sweet basil infected with Peronospora belbahrii at 5dpi
Four-week old sweet basil Dolly plants were inoculated with a Hawaii isolate of Peronospora belbahrii by spraying sporangial suspension on the first pair of true leaves. The inoculated leaves were collected at 5 days post inoculation. Total RNA was isolated using RNeasy Plant Mini Kit (Qiagen) and contaminating gDNA was removed using DNA-free kit (Thermo Fisher Scientific). mRNA was purified from total RNA and libraries with 280 bp inserts were constructed using TruSeq RNA library Prep Kit v2 (Illumina), and the whole transcriptomes were sequenced using the 100 bp paired-end mode on a HiSeq 2500 (Illumina)
Dolly5d