GSM5068645: ARID1A_EGF_rep2; Mus musculus; ChIP-Seq
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with ARID1A antibody. ChIP-seq samples were prepared in duplicates per condition. Library for ChIP-seq was prepared using Microplex Library Preparation V2 following the manufacturer's instruction. 0.5 ng double-stranded ChIP-DNA was utilized as start material, repaired into blunt ends and subsequently ligated with stem-loop adaptors. Libraries were amplified and size-selected by AMPure beads. Library quantification was performed using Agilent Bioanalyzer 2100. Finally, library was prepared as 2 nmol/L for sequencing.