GSM4859725: Kupffer Cells + IL2 - rep2; Mus musculus; RNA-Seq
Cells were lysed in ReliaPrep™ RNA Cell Miniprep System (Promega #Z6011) and total RNA was isolated following manual extraction. DNA digestion was performed with TURBO DNA-free™ Kit (Invitrogen #AM1907). SMART-seq Ultra Low Input 48 library protocol NovaSeq 6000 SP Reagent Kit 100xSE (100 cycles)