GSM4983126: Sham-WT; Mus musculus; RNA-Seq
Hearts from wildtype and Nrf1 knockout mice were dissected in ice-cold PBS and a transverse cut on the ligation plane (for MI hearts) or similar level (for sham hearts) was made. Heart tissue below the ligation plane was collected and pooled from 4-6 hearts of the same genotype for nuclei isolation.  Cardiac nuclei isolation was performed as previously described (PMID:33111095) Total cardiac nuclei were used to generate single nucleus RNA-seq libraries using Single Cell 3' Reagent Kits v2 (10xGenomics) according to the manufacturer's protocol single-nucleus RNA-seq