GSM4860395: Fresh-frozen canine oligodendroglioma - dog no. 60636; Canis lupus familiaris; RNA-Seq
RNA extraction using the QIAGEN RNeasy® Mini Kit The RNA-seq libraries were prepared using the Lexogen CORALL Total RNA-Seq Library Prep Kit which generates labelled with 12nt long Unique Molecular Identifiers (UMIs), forward stranded libraries for the transcriptome analyses. After the library preparation, the samples were amplified by PCR and finally purified to remove PCR components that could interfere with further RNA-seq quantification. Then, paired-end reads were generated by the Illumina NovaSeq 6000.