GSM4984050: LungD7_1; Homo sapiens; RNA-Seq
Micropatterned coverslips with 225-μm diameter synthetic lung buds at day 7 of lung induction were dissociated with TrypLE Express (Gibco) for 10 min at 37 °C. Samples were loaded for capture with the Chromium System using the Single Cell 3′ v3 reagents (10X Genomics). Following cell capture and lysis, cDNA was synthesized and amplified according to the manufacturer's instructions (10X Genomics). The resulting libraries were sequenced on the Nova-seq platform.