GSM6005244: S2_F_H; Mus musculus; RNA-Seq
GSM6005244
Total RNA was extracted using Qiagen RNeasy Mini kits. Quantity and quality were determined using a NanoDrop™ spectrophotometer and an Agilent 2100 BioAnalyzer. Samples were enriched for poly-A RNA using the Invitrogen Dynabeads mRNA Purification kit. Paired-end (2x150, expected size of 300 bp), strand-specific, cluster-ready libraries were prepared from the poly-A enriched RNA using the NEBNext Ultra II RNA Library Prep kit.