GSM4984073: Il12rb2fl/fl Aldara rep2; Mus musculus; RNA-Seq
Total RNA of IF keratinocytes was isolated using the Qiagen RNeasy Micro kit according to the manufacturers' protocol. Library preparation was performed according to illumina`s “smartseqII” protocol (Picelli et al., 2014). The Novaseq 6000 (Illumina, Inc, California, USA) was used for cluster generation and sequencing according to standard protocols. Sequencing was single end 100bp.