GSM6202889: Mouse_BCC_CD45.2+; Mus musculus; RNA-Seq
GSM6202889
Tumors were dissociated by first mincing into pieces smaller than 1 mm in diameter and then incubated in 40 mL of a 0.5% collagenase solution (Gibco; 25200056) for 1hrs with rotation at 37°C . 5 mL of 0.25% Trypsin was then added and incubated for an additional 15min with rotation at 37°C . For Sample1/Mouse_BCC_CD45+_Primary_Tumor  cells were FACS sorted for CD45+ cells. For Sample 2/Mouse_BCC_CD45.2+ and Sample 3/Mouse_BCC_CD45.2-, cells were  FACS sorted for CD45+CD45.2+ or CD45+CD45.2-. Cells were then processed using the 10X Genomics Next GEM scRNA-Seq solution. Library construction followed the 10X Genomics Next GEM scRNA-Seq pipeline.