GSM6202887: Wound_Trem2+_Rep2; Mus musculus; ATAC-seq
GSM6202887
Samples were dissociated by first mincing into pieces smaller than 1 mm in diameter and then incubated in 40 mL of a 0.5% collagenase solution (Gibco; 25200056) for 1hrs with rotation at 37°C . 5 mL of 0.25% Trypsin was then added and incubated for an additional 15min with rotation at 37°C . Cells were then FACS sorted for Cd45+Trem2+ cells and then processed for bulk ATAC-Seq. Bulk ATAC-Seq was performed using the Omni-ATAC protocol (Corces et al., 2017: 28846090)