TCR sequencing of human adipose-derived MSC recruited T cells
Hu-mice were reconstituted with human PBMCs. PBMCs from the same donor were collected in TRIzol (5106 cells/ml) and stored at -80C as a control (PBMC group). Two weeks later, one hu-mouse was subcutaneously administered a mixture of human Ad-MSCs (1107 cells per mouse) or PBS with Matrigel (BD, USA) separately on the dorsal surface of the right and left sides; a total of 10 hu-mice were subjected to the same treatment. Seven days later, all 10 hu-mice were sacrificed, and the administered cells were isolated. Tissues containing MSCs or PBS were gathered separately in TRIzol as MSC or PBS groups, frozen in liquid nitrogen and stored at -80C before TCR repertoire sequencing.
GENEWIZ_0