GSM6008332: Hypoxia sample1 [Hypo-Y-1_ATAC-seq]; Mus musculus; ATAC-seq
GSM6008332
ATAc-seq was performed on 50K cells per sample, as described previously (Buenrostro et al., 2013). In brief, cells were harvesred, washed with PBS, resuspended in ice-cold lysis buffer and centrifuged for 10min at 500g at 4C. Nuclei were then incubetaed with the transposition reaction mix, for 30 minutes at 37C, shaking at 250rpm. DNA was purified using the DNA concentrator kit from Zymo Research. Following DNA purification libraries were prepared using Nextera PCR primers and the NEBNext High-Fidelity PCR Master Mix. Libraries were PCR amplified for 5 cycles. DNA concentration of the libraries was measured with Qubit and library quality was assessed by running samples on the TapeStation.