Plant sample from primary maize root:  zone of the apical meristem (0-1 mm) from cap junction
Root zones pooled from 30-40 plants (an average) were collected separately into plastic tubes with liquid nitrogen and stored at -80. Total RNA was isolated from plant samples using the Trizol extraction method combined with RNeasy Plant Mini Kit (Qiagen) according to the manufacturers instructions. The quantity and quality of RNA was controlled using NanoPhotometer NP-80 Touch (Implen, Munich, Germany). RNA integrity test (all samples had a RIN value higher than 7), cDNA library construction, and sequencing were performed by the Novogene Corporation (China; https://en.novogene.com/).  Raw reads from run were filtered to remove adapter sequences, contamination and low-quality reads.
Zma_MER5