Whole genome sequencing of Saccharomyces cerevisiae WS84-20
WS84-derived isolates were grown on LG (3% raffinose and 0.005% galactose) plates from a single cell to a colony (roughly 25 cell divisions) for two cycles, these independent colonies were purified on HG(3% raffinose and 0.05% galactose) plates. The  purified colonies were grown on high-gal plates. The yeast cells were collected for genomic DNA extraction and whole genome sequencing by Illumina technology.
WS84-20