GSM5068724: J-Lat 10.6 TNFα rep2; Homo sapiens; RNA-Seq
Cells within each group were removed supernatant and washed once with PBS. Total RNAs were extracted with TRIzol reagent. The qualities of these RNAs were evaluated by Nanodrop (ThermoFisher) and BioAnalyzer 2100 (Aglient). The RNA-Seq libraries were performed with TruSeq Stranded mRNA Library Prep Kit (Illumina) and sequenced with HiSeq X Ten (Illumina) under PE150 protocol.