Illumina short reads: WGS
For Illumina PCR-free sequencing, total genomic DNA of FAFU-HL-1 was extracted using a Qiagen DNeasy Plant Mini kit following the manufacturers instructions (Qiagen, Valencia, CA). PCR-free sequencing libraries were generated using Illumina TruSeq DNA PCR-Free Library Preparation Kit (Illumina, USA) following the manufacturers recommendations. After quality assessment on an Agilent Bioanalyzer 2100 system, the libraries were sequenced on an Illumina platform (NovaSeq 6000) by Beijing Novogene Bioinformatics Technology Co., Ltd. (hereafter referred to as Novogene).
FAFU-HL-1-W