GSM4861637: Sample_103946; Mus musculus; RNA-Seq
Femurs were dissected, distal epiphyseal growth plates were removed and proximal ends were cut off. Remaining bones were cut roughly, and cells were enzymatically dissociated by collagenase. GFP+ cells (Control) or GFP+tdTomato+ cells (∆CXCL12) were FACS-isolated. ~20,000 cells were used for subsequent steps. Nextera XT DNA Library Preparation Kit