GSM5068593: P300KO_ATACseq_Rep4; Mus musculus; ATAC-seq
Cells were defrosted, washed twice in PBS and 50,000 viable HSPCs were then processed following the OMIM-ATAC-Seq protocol from Corces et al., Nature Methods 2017 with a dead cell pre-cleanup step with DNase I (Worthington cat#LS002007, 30 min at 37°C) followed by two cold PBS washes. Libraries were prepared utilizing 2xTD buffer (15027866, Illumina) and Robust Tn5 Transposase (EMQZ1422, Creative Biogene) and cleaned up with a Zymo DNA Clean and Concentrator-5 kit (cat#D4014). OMIM-ATAC-Seq libraries were amplified with a total of 11 PCR cycles with ATAC-Seq primers (Buenrostro et al., 2015 (PMID: 25559105). Paired-End, 100bp sequenced on an Illumina NOVASeq.