GSM5219305: OSKM_All_D09_b1 (ATAC-seq); Mus musculus; ATAC-seq
The samples were purified using Qiagen MiniElute kit (QIAGEN, 28204), Transposed fragments were directly PCR amplified and sequenced on an Illumina NextSeq 500 platform to generate 2 × 36-bp paired-end reads. ATAC-seq library preparation was performed as previously described (Buenrostro et al., 2013). Briefly, cells were trypsinized and 50,000 cells were counted and incubated in lysis buffer to isolate nuclei. Nuclei were then resuspended in transposase reaction mix for 30 min at 37 °C (Illumina, Fc-121-1030).