RNA-Seq
Paired-end sequencing of the amplicons and the processing of sequening data were carried out. The software FastQC v0.11.8 was used to check and verify the paired-end reads. After merging the sequences, short reads (less than 305 base pairs). The amplicon sequences were processed with QIIME 1.9.1. The sequences were dereplicated and verified for chimeric sequences (de novo). The sequences were clustered into operational taxonomic units (OTUs) with 97% identity. The taxonomic classification of the OTU sequences was also carried out with QIIME 1.9.1 against the SILVA database 132 using the assignment method with chloroplasts, extrinsic domains and unclassified OTUs being eliminated from the data set.
V04-01-BR1-1_R1/R2