GSM5572942: RNAse1_smallRNA; Arabidopsis thaliana; ncRNA-Seq
Following trypsin and RNase treatments,  RNA was extracted using a Trizol/chloroform protocol. sRNA libraries were constructed as described using the RealSeq-AC kit (no. 500-00048; RealSeq Biosciences), following the manufacturers' recommendations.  In order to capture all types of small RNAs, we used 1ug of RNA as starting material. RNAseq libraries were generated using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB #E7765; New England Biolabs), using 500 ng of total RNA as starting material. All ibraries were sequenced on a NextSeq 550 instrument with single- end 75-bp reads at the Delaware Biotechnology Institure (DE, USA). small RNA and RNA-seq libraries were sequenced on an Illumina NextSeq 550 instrument with single- end 75-bp reads at the Center for Genomics and Bioinformatics, Indiana University, Bloomington, IN USA.