RNA-Seq of homo sapiens: adult male dental pulp
Total RNA was extracted from cells by using Trizol reagent, and 2 g of the RNA for each sample was used as input material for rRNA removal using a Ribo-Zero rRNA Removal Kit (RZH1046, Qiagen) according to the manufacturers instructions. Library construction and sequencing were performed by Annoroad Gene Technology (Beijing, China). Pair-ended RNA-seq data was generated using the Illumina HiSeq X Ten platform for shNC-hDPSCs (3 lines), shITG6-hDPSCs (3 lines) . The pair-ended sequencing reads were aligned to human genome using Star Aligner (version 2.5.1b).
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