GSM5453434: pool_2_48os; Mus musculus; OTHER
Genomic DNA was extracted from frozen retina and liver samples. 150 bp regions flanking the on target and off-target sites of mRho and mAlb gRNA were amplified by PCR, pooled and used for library construction. 10 ng of PCR products were used as input for the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina OTHER: Amplicon sequencing