GSM5693449: BM-RNA-055_TK3_V1-deleted-DNA/DNA/ALVAC/ALVAC+gp120_Week 0; Macaca mulatta; RNA-Seq
RNA isolation was performed using RNeasy Micro Kit (Cat#74004, Qiagen) following manufacturer instructions. Briefly, CD14+ cells in RNAlater were thawed on ice, 500 µl PBS was added to each vial, and centrifuged at 3000 rpm for 10 min at 4ºC. Supernatants were discarded while cell pellets were processed following manufacturer protocol and resuspended in a final volume of 14 µl of water. The library preparation was done using the NEBNext Ultra II Directional RNA Library Prep for Illumina kit (cat # E7760L, New England BioLabs) and following manufacturer instructions. The final product was quantitated by qPCR before cluster generation and sequencing.