GSM4861290: N13; Homo sapiens; RNA-Seq
Explanted whole human hearts were transported on ice to the Massachusetts General Hospital. Organs underwent gross pathologic examination, including measurements of heart weight, left atrial and left ventricular wall thickness. Left ventricular free wall was excised and total RNA was extracted using the Nucleospin RNA isolation kit (Clontech). cDNA libraries were generated using SMART-seq2 and NExtera XT (Illumina). Sequencing was performed with paired-end configuration on Nextseq 500.