GSM5693652: Pool 4 TCR: Sorted CD3_TCR_Tissue_Pool4; Homo sapiens; RNA-Seq
GSM5693652
Matched Colonic biopsy (Tissue) and Peripheral Blood (Blood) samples from Healthy controls, patients with inflamed Ulcerative Colitis (UC_I) and Checkpoint inhibitor-induced colitis (CC_I) were defrosted. Tissue was digested to yield high viability single cells, and then tissue and blood were put through flow-cytometry (FACS) sorting to enrich for T cells, before loading on to the 10X platform for single-cell RNA sequencing Samples were loaded onto the 10X sc-RNA seq platform, with GEM generation and RNA RT, followed by generation of Gene expression (GEX), Antibody tagged oligo (ADT - with CITE-seq proteins and hashing information) and T-cell receptor (TCR) library amplification and indexing, as per protocol CG000208, Rev F