Nanopore of Carica papaya:SunUp
After the sample was qualified, long DNA fragments were selected using the BluePippin system (Sage Science, USA). Next, DNAs were repaired and the DNA ends were prepared for adapter attachment. Then attach sequencing adapters were supplied in the SQK-LSK109 kit to the DNA ends. Finally, Qubit 3.0 Fluorometer (Invitrogen, USA) was used to quantify the size of Library fragments.
papaya nanopore of SunUp