GSM6304419: ATAC-seq_iMK-D3-rep1; Homo sapiens; RNA-Seq
GSM6304419
For scRNA-seq, freshly sorted EBs (day 0) and viable cells from the iMK induction culture on days 3, 5, and 7 were collected and analyzed using the Chromium system (10x Genomics) according to the manufacturer's instructions for an expected capture rate of approximately 8,000 single cells per sample. For ATAC-seq, cells were dissociated from each pooled sample to obtain single-cell suspensions. Then, cells were suspended in nuclear isolation buffer and washed repeatedly using nuclear wash buffer following a standard nuclear isolation protocol. A total of 40,000 nuclei were pelleted and re-suspended with transposase for 30 min at 37℃. The transposed DNA was purified with a MinElute Kit (Qiagen Inc., Valencia, CA, USA) and was used to generate the library via PCR amplification.