NextSeq 500 paired end sequencing; RNA-seq for evaluation of the disruption of pluripotency and the related differentiation program in mouse embryonic stem cell (mESC) models
RNA-seq for evaluation of the disruption of pluripotency and the related differentiation program in mouse embryonic stem cell (mESC) models
12_BRSG_in_vivo_PGCs_rep2_p
Mouse PGCs (stella-GFP positive cells) at embryonic day 9.5 were sorted for the following RNA-seq experiment by using the fluorescence-Activated Cell Sorting (FACS) Analysis. Total RNA was isolated using a NucleoSpin RNA Mini kit (MACHEREY-NAGEL) and then, mRNA was isolated using NEB Next Poly A m-RNA magnetic bead selection kit (E7490S).  RNA-seq library was prepared using NEBNext Ultra II RNA Library Prep Kit for Illumina (E7770).