GSM5503573: scRNA-seq of HEB59; Mus musculus; RNA-Seq
Fresh bone marrow cells were harvested from mouse femur and tibia. They were treated with red cell lysis. Samples were divided into two samples (half for bulk bone marrow and half for lineage negative selection). Lineage negative cells were isolated with Miltenyi Biotec kit (130-090-858) and stained with anti-biotin. Live cells were sorted from bulk bone marrow samples or live, biotin-negative cells were sorted from lineage negative samples. 5000 bulk and 5000 lineage negative were pooled together for library preparation. 10X Genomics Single Cell RNA version 3.1