GSM6139169: WI-38, etoposide treated, scRNA-seq; Homo sapiens; RNA-Seq
GSM6139169
Etoposide-treated WI38 cells were trypsinized, washed and resuspended in PBS, and subjected to single-cell GEM (Gel Bead-Emulsion) generation with Chromium Controller (10x Genomics) scRNA-seq library was performed according to the manufacter's instructions (10x Genomics Chromium Next Gem Single Cell 3' Regent Kits v3 protocol, 10x Genomics). In brief, single cell suspension was loaded into a Chromium Next GEM Chip G (10x Genomics), and GEMs were generated using the Chromium Single Cell Controller (10x Genomics). We used 11 and 13 cycles for cDNA amplification and Sample Index PCR (Single Index Kit T Set A, 10x Genomics), respectively