NextSeq 500 paired end sequencing; RNA-seq for evaluation of the disruption of pluripotency and the related differentiation program in mouse embryonic stem cell (mESC) models
RNA-seq for evaluation of the disruption of pluripotency and the related differentiation program in mouse embryonic stem cell (mESC) models
06_ZHBTc4_d0_rep2_p
T(Brachyury)-inducible ZHBTc4 mESCs were cultured in 2iLIF system. T was induced by the stimulation with dexamethasone and Oct4 was deleted by the stimulation with doxycycline for 24 hr. Then, cells were collected for the following RNA-seq experiment. Total RNA was isolated using a NucleoSpin RNA Mini kit (MACHEREY-NAGEL) and then, mRNA was isolated using NEB Next Poly A m-RNA magnetic bead selection kit (E7490S).  RNA-seq library was prepared using NEBNext Ultra II RNA Library Prep Kit for Illumina (E7770).