GSM5949989: replicate2 for   GSC827 tumor; Homo sapiens; RNA-Seq
GSM5949989
5000 cells from subconfluent GSC827 (matched for density and media changes) were loaded on a a GemCode Single Cell Instrument (10x Genomics) to generate single-cell GEMs for library preparation using Single Cell Gel Bead Kit (P/N 120217) according to manufacturer's guidelines. G0/G1 cells were first sorted on an FACSAria II (BD Biosciences) for the cells postive only for mCherry-hCDT1(aa30/120). RNA libraries were prepared for sequencing using Single Cell Library Kit from 10x Genomics (P/N 120218) according to manufacturer's guidelines.