GSM5849416: SC_high_passage; Homo sapiens; RNA-Seq
GSM5849416
Cells were washed with PBS and detached using trypsin. The enzyme was quenched with media containing 2% FBS and washed away by extra media followed by PBS. Cells were passed through a 37μm reversible cell strainer and put on ice. Before submission for library prep. snRNA-seq libraries were prepared with Chromium Next GEM Single Cell 3′ Kit v3.1 (10x Genomics). The libraries were sequenced at UCSF GenomicsCoLab.