GSM5503420: ChIPseq_IgG_bte1_rep2; Arabidopsis thaliana; ChIP-Seq
2 g of 9-day-old seedlings grown on MS medium were collected and cross-linked with 1% formaldehyde for 20 min under vacuum and then ground into a fine powder in liquid nitrogen. Chromatin was isolated and sheared into fragments (200–500 bp) by sonication. The sonicated chromatin was incubated with antibody-bound Sera-Mag SpeedBeads Protein A/G Magnetic Particles (17152104010150, GE Healthcare) overnight at 4℃. Precipitated DNA was then reverse-crosslinked and digested with RNase A and Proteinase K, after which it was purified with the ChIP DNA Clean & Concentrator kit (D5205, Zymo research) according to the manufacturer's instructions. All ChIP-seq libraries were generated with the NEBNext® Ultra II DNA Library Prep Kit for Illumina® (E7645S, NEB) according to the manufacturer's instructions.