Amplicon sequencing of human cytomegalovirus UL97 or UL54 genes: adult homo sapiens
Purified DNA was quantified using Quant-iT PicoGreen dsDNA Kit (Invitrogen, Australia), with fluorescence measured on VictorTM X2 plate-reader (PerkinElmer, Australia). Dual-indexed sequencing libraries were prepared using DNA Prep v2 (Illumina, Australia). Size distribution of library was assessed on LabChip GX Touch24, using HTDNA High Sensitivity Assay (PerkinElmer, Australia). Samples were pooled in equimolar quantities and spiked with 10% PhiX. Sequencing was performed using MiSeq Reagent Nano v2 on MiSeq Sequencer (Illumina, Australia).
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