GSM5411960: Liver_H2O_InulinUn_RNA_rep2; Mus musculus; RNA-Seq
Total RNA was extracted from aliquots of frozen tissues using the RNeasy kit (Qiagen).  Homogenization was carried out in the provided lysis buffer using bead beating (Precellys homogenizer, Bertin Technologies). The NEBNext Ultra or Ultra II Directional RNA Library Prep Kit for Illumina with the mRNA magnetic isolation module (New England Biolabs) was used to prepare sequencing libraries from 1 µg of total RNA.