GSM5289417: 5029_AM; Mus musculus; RNA-Seq
Pelleted airway macrophages were directly lysed with 350 μL of Buffer RLT, and total RNA was isolated using the Qiagen RNeasy Micro Kit per manufacturer's instructions. Stranded libraries were prepared using 10 ng of total RNA with the NuGEN Ovation SoLo RNA-Seq Library Preparation Kit.