GSM5145842: F4/80+ liver macrophages WT; Mus musculus; RNA-Seq
Liver were flushed with PBS, scissor-minced, then digested with collagenase D for 20 min at 37°C. After centrifugation at 600 g for 10 min, stromal cells were separated in 30% percoll and pellets containing stromal cells were incubated with erythrocyte lysis buffer, then CD45+/F4/80+ cells (pool of 6 animals per genotype) were sorted by flow cytometry. Single cell libraries were prepared with the Chromium Single Cell 3' Library & Gel Bead Kit v2 & i7 Multiplex Kit and A Chip Kit from 10X Genomics. The barcoded library was sequenced on an Illumina HiSeq3000 in 150-8-150 paired-end configuration