GSM4861235: LE85N_YELLOW; Homo sapiens; ncRNA-Seq
Normal density neutrophils were isolated by dextran sedimentation from control and SLE patients. LDG was isolated by negative selection from the PBMC fraction. Cells were allowed to spontaneusly NET. RNA was isolated from NETs using the ExoMir Kit from BIOO Scientific (catalog #5145). cDNA libraries were prepared using the protocol described by Hafner et al. 2012 Methods 58(2): 164-170. Sequencing data were generated on Illumina HiSeq2500 instrument according to the manufacturer's instructions