GSM5149205: RNA T KO Day2; Mus musculus; RNA-Seq
Total RNA was extracted from 40,000 mESCs or differentiated cells using Trizol reagent (ThermoFisher; 15596036) following the manufacturer's instructions and purified using the RNeasy Micro kit (Qiagen). Residual genomic DNA was digested on a column. Strand-specific RNA-seq libraries were generated from 100 ng of total RNA using the ScriptSeq v2 (Epicentre) low input library preparation kit according to manufacturer's instructions. The library was amplified using 15 PCR cycles. RNA-seq libraries were quantified using the Qubit HS DNA assay (Life Technologies) and the size distribution was assessed using the DNA HS Bioanalyzer chips (Agilent).