GSM5217198: HEK293T siCtrl TGIRT small RNA rep1; Homo sapiens; ncRNA-Seq
Total RNAs was extracted from cell lines by TRIzol Reagent (Invitrogen #15596018) and Direct-zol RNA Miniprep Plus Kit with on-column DNase treatment (ZYMO #R2071). Small RNA-seq library preparation was performed using NEBNext Small RNA Library Prep Set for Illumina (NEB #7330) with modification to use TGIRT-III RT enzyme (InGex). The resulting amplified libraries were size selected on 8% TBE polyacrylamide gel to enrich for insert of 15-50 nucleotides long RNA.