GSM5219079: Fli1 KO Notch1 OE Lineage depleted cells2; Mus musculus; RNA-Seq
Lineage negative CD45.2 positive HSPCs were isolated by fluorescence-activated cell sorting and subjected to RNA extraction using Arcturus PicoPure RNA isolation kit (Applied Biosystems). RNA-seq cDNA libraries were constructed using the 10X Chromium single-cell 3′ Library Kit according to the manufacturer's original protocol.