GSM5218723: sham blood 2; Mus musculus; RNA-Seq
500-1000 cells were sorted to a plate with lysis buffer. cDNA was generated from cell lysis using the SMART-Seq Ultralow Input RNA Kit (Qiagen). The sequencing library was constructed by following the Illumina Nextera XT Sample Preparation Guide. One nanogram of input cDNA was tagmented (tagged and fragmented) and amplified using the Illumina Nextrera XT kit. Sequence libraries of each sample were finally equimolarly pooled and sequenced on an Illumina Nextseq 500 system, using a paired-end 75-bp strategy.