GSM5502340: Sample 24 Arabidopsis thaliana pifq seedlings; Arabidopsis thaliana; ChIP-Seq
For each replicate, 2.5 gr of 2-day old wild-type and pifq D, R1h and WLc treated seedlings were processed as described in (Gendrel et al., 2005) using 5 µgr Diagenode Ab C15410004 H3K9ac antibody and a mix of 25/25 µl of protein-A/G dynabeads (Invitrogen). 5-10 ng of DNA per sample were used for library preparation with Accel-NGS 2S Plus DNA Library Kit (Swift Biosciences) and 9 PCR cycles. 300-700 bp fragments were purified