RNA-Seq of Leishmania donovani
RNA sequencing libraries were prepared using the Spliced leader sequencing (SL-Seq) method as described in Cuypers et al. (2017). This protocol makes use of the presence of the affixed 39 nucleotide sequence spliced-leader (SL) that is present at the 5 end of all functional Leishmania mRNAs. RNAs containing a SL are selectively amplified with the protocol, and adapters required for Illumina sequencing are ligated. The SL-Seq libraries were 1 X 50 bp sequenced with the HiSeq 1500 platform
Int_RNA_575_BR4