GSM5575026: WT no drug; Homo sapiens; RNA-Seq
Total RNA was isolated with Direct-zol-96 RNA purification kit (Zymo Research) following manufacturer's instructions. The recommended in-column DNase I treatment was also conducted to remove genomic DNA. Purified RNA was quantified with a microplate reader (Tecan M200 Infinite Pro) using the Quant-iT RNA assay kit (ThermoFisher). Ribosomal RNA was removed using the NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (New England Biolabs). Sequencing libraries were prepared using the NEBNext Ultra II RNA Library preparation kit for Illumina (New England Biolabs) and the pooled library was loaded in a NextSeq500/550 Mid Output kit v2.5 (Illumina) for sequencing in a NextSeq500 (151 cycles, 8 nucleotides of index).