GSM5411841: input_3_GFP; Mus musculus; RNA-Seq
Input RNA for transcriptome studies was recovered from liver tissue lysed in polysome buffer using Trizol LS and RNeasy mini kit (Qiagen) including on column DNase digestion. For translatome studies, RNA was recovered from corresponding polysome fractions by Trizol-LS extraction, followed by chloroform-isopropanol-ethanol precipitation, heparinase treatment (New England Biolabs, 15 minutes, 37°C), and RNA cleanup step using RNA Clean & Concentrator (Zymo). RNA libraries were prepared for sequencing using standard Illumina protocols.