GSM5069252: WT_Bm rep2; Mus musculus; RNA-Seq
Femur and tibia were dissected and bone marrow were flushed, then cells were layered on Ficoll-paque (GE Healthcare) and centrifuged. Cells at the interface were collected and washed twice with ice-cold PBS.For the quality check and counting of single cell suspension, the cell survival rate is generally above 80%. The cells that have passed the test are washed and resuspended to prepare a suitable cell concentration of 700~1200 cells/ul for 10x Genomics ChromiumTM. Generation of gel beads in emulsion (GEMs), barcoding, GEM-RT clean-up, complementary DNA amplification and library construction were all performed as per the manufacturer's protocol. 