GSM5746424: TUL_010_01; Homo sapiens; RNA-Seq
Total RNA isolation was performed using the RNeasy Mini Kit (Qiagen) according to the manufacturers' instructions Library preparation was performed using an NEBNext Ultra Directional RNA Library Prep Kit (New England Biolabs). Libraries were validated via Bioanalyzer (Agilent Technologies) to determine size distribution and concentration. Validated libraries were then sequenced at Tsukuba i-Laboratory LLP (Tsukuba, Japan) on a NextSeq500 (Illumina) with the paired-end 36-base read option.