GSM4860412: PABP IP Rep2; Drosophila melanogaster; OTHER
Two hundred flies (100 male and 100 female) of each sample were ground into a fine powder, exposed to UV, lysed in the lysis buffer, immunoprecipitated PABP using magnetic FLAG-beads and isolated RNA using the Trizol reagent. For total input libraries, RNA was isolated from total extracts prior to immunoprecipitation using the Trizol reagent. RNA libraries were prepared for sequencing using the Ovation SoLo RNA-Seq System (Tecan Genomics, S02240). CLIP-Seq