GSM5068522: Control_P10_RNAseq_rep3; Mus musculus; RNA-Seq
Dentate gyrus were dissected from control or MDZ-exposed Nestin-EGFP mice at P10 and 8w and then EGFP-positive cells were isolated by FACS. mRNAs were isolated from the cells for the construction of sequencing libraries using Dynabeads mRNA DIRECT Micro Kit (Invitrogen) following the manufacturer's protocol. A cDNA library was constructed by 50,000 cells isolated from one mouse at P10. Three to five mice (approximately 30,000 cells) were used for cDNA library preparation from 8w mice.  For overexpression of Egr1, cultured mouse NSCs were infected with M2rtTa-expressing lentivirus constructs together with or without Flag-tagged Egr1-expressing lentivirus constructs (Control or Egr1) and then isolated mRNA for the construction of sequencing libraries using Dynabeads mRNA DIRECT Micro Kit. Purified mRNAs were subjected to cDNA library construction using a NEB Next Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturer's protocols.