Shotgun metagenomic sequencing of gut homogenate from swine with L. intracellularis infection
DNA and RNA extraction was performed using MagMax Core Nucleic Acid Purification Kit (ThermoFisher). cDNA was synthesized from RNA with the Maxima H Minus Double-Stranded cDNA Synthesis Kit (Thermo Fisher). Library preparation was performed with the Nextera XT kit (Illumina) and sequencing performed on MiSeq sequencer with 2x300 bp V3 chemistry (Illumina).
20-00251-1_S13