MiSeq of Stool Metagenome: Amazona vittata
DNA was extracted from 21 stool samples from multiple captive Amazona vittata specimens using the FastDNA Spin Kit for Soil (MP Biomedicals). DNA was pooled and a genomic library was prepared using the Nextera DNA Flex Library Preparation kit. Adapter sequences were added through a limited-cycle PCR. The library was pair-end-sequenced using the MiSeq Reagent Kit v3 for 600 cycles (Illumina).
Avittata_metagenome