GSM6008184: 3_TNF-α-induced chondrocytes_Ctrl; Rattus norvegicus; RNA-Seq
GSM6008184
Total RNA was extracted from the chondrocytes using Trizol (Invitrogen, Carlsbad, CA, USA) according to manual instruction. Total RNA was qualified and quantified using a Nano Drop and Agilent 2100 bioanalyzer (Thermo Fisher Scientific, MA, USA). Oligo (dT)-attached magnetic beads were used to purified mRNA. The final library was amplified with phi29 to make DNA nanoball (DNB) which had more than 300 copies of one molecular, DNBs were loaded into the patterned nanoarray and single end 50 bases reads were generated on BGIseq 500 platform (BGI-Shenzhen, China).