GSM5218511: ChIP-seq_E16.5_H3K9cr-rep2; Mus musculus; ChIP-Seq
Standard Native Chromatin Immunoprecipitation (N-ChIP) was employed to extract chromatin and perform immunoprecipitation, the IPed DNA was extracted by the phenol-chloroform method. ChIP's DNA was subjected to end-repair and then was 3'adenylated. Adaptors were ligated to the ends of these 7'adenylated fragments. Next, polymerase chain reaction amplification was to amplify those fragments with adaptors from previous step.