GSM5694088: FTOc_BF3_rep1_scRNAseq; Mus musculus; RNA-Seq
Mouse brains were dissected after trans cardiac perfusion with cold 1x DPBS, and coronally sliced to obtain 1 mm sections. The ARC-ME region was microdissected in cold 1x DPBS and nuclei were isolated using a pre-chilled Dounce tissue grinder and iodixanol gradient as described previously with the addition of 200 units of RNasin Plus Ribonuclease Inhibitor (Promega) in the homogenization buffer. The isolated nuclei were immediately used for single cell MultiOME ATAC + Gene Expression kit according to the manufacturer's instructions.