GSM6202405: MOUSEPOOLEDNORMAL; Mus musculus; OTHER
GSM6202405
For IMPACT.KPC tumor cells from 2 weeks tumor bearing liver were directly sorted into 1.5ml Eppendorf tube  bearing liver were directly sorted into 1.5ml Eppendorf tube precoated with 10% BSA. DNA samples were extracted with QIAamp DNA Micro Kit,and submitted to the Integrated Genomics Operation (IGO) at MSKCC for quality and quantity analysis, library preparation and sequencing. For IMPACT, 89-100ng of mouse genomic DNA were used for library construction using the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) with 8 cycles of PCR. After sample barcoding, 100-110ng of each library were pooled and captured by hybridization with the mm_IMPACT (Integrated Mutation Profiling of Actionable Cancer Targets) assay, which captures all protein-coding exons and select introns of 578 cancer-related genes. Capture pools were sequenced on the HiSeq 4000, using the HiSeq 3000/4000 SBS Kit (Illumina) for PE100 reads. Following these criteria, the average coverage was 576X, with an average of 99% of the targeted sequences covered 30X.