control temperature-mock-1
We designed a novel targeted DNA capture technique to capture a user-defined panel of 35 targets with ~550 bp from Arabidopsis genome. In brief, flap oligos for each target formed flap structures in target regions, which were cleaved by thermo-stable FEN1. Universal adaptors were added to two ends of target fragments using thermo-stable ligase. The captured DNA were subjected to enzymatic methylation and PCR amplification for the PacBio Sequel II long-read sequencing. The circular consensus (CCS) reads were generated from at least 5 passes in a single polymerase read with default settings of other parameters.
BCA1004