RNA-Seq of Hediste diversicolor: adult middle body
Total RNA extractions from the head and the middle body (reciprocal distance from the head and the tail) were performed by NZY Total RNA Isolation kit (NZYTech, Lda. - Genes and Enzymes) following the manufacturers recommendations. Strand-specific libraries were built by Macrogen, Inc. These libraries included inserts between 250 and 300 bp, and were sequenced on the Illumina HiSeq4000 platform using 150 bp paired-end reads. The raw datasets (head and middle body) were inspected using the FastQC software (version 0.11.8), quality-filtered with Trimmomatic (version 0.38) software and corrected for random sequencing errors with Rcorrector (version 1.0.3).
Hdiv2