GSM4860406: FMRP smInput; Drosophila melanogaster; OTHER
Two hundred flies (100 male and 100 female) of each sample were ground into a fine powder, exposed to UV, lysed in the lysis buffer and immunoprecipitated FMRP using magnetic FLAG-beads. Subsequently, RNA from the IP and size-matched input were isolated as described in the method described in Nostrand et al., 2016. RNA libraries were prepared for sequencing using the method described in Nostrand et al., 2016. Enhanced CLIP-Seq