Bulk RNA-seq analysis of iWAT APCs from WT mice
Inguinal WATs from WT mice were washed with PBS, minced, and digested with type II collagenase (2 mg/ml) in KRBB containing 10 mM HEPES (pH 7.4) and 3% BSA for 1 hr at 37C. The digested tissues were filtered through 70-m and 40-m cell strainers (BD Biosciences) and then centrifuged at 500 g for 5 min. The cell pellet was resuspended in PBS for FACS sorting.  CD45-CD31-CD34+CD29+Sca1+ cells were sorted using BD Aria II Sorter and performed bulk RNA sequencing.
APC_3