GSM5068365: Plasma exosome_R0_rep1; Homo sapiens; miRNA-Seq
A total of 4mL of plasma was mixed with RiboTM Exosome Isolation Reagent, and exosome isolation was performed according to the manufacturer's instructions (Ribobio, China). Exosomal RNA was extracted by the HiPure Liquid Plasma miRNA Kit (Megan, China). The quantity and integrity of Exosomal RNA yield were assessed by using the Qubit®2.0 (Life Technologies, USA) and Agilent 2200 TapeStation (Agilent Technologies, USA) separately. 50ng Exosomal RNA of each sample was used to prepare small RNA libraries by NEBNext® Multiplex Small RNA Library Prep Set for Illumina (NEB, USA) according to manufacturer's instructions. The libraries were sequenced by HiSeq 2500 (Illumina, USA) with single-end 50bp at Ribobio Co. Ltd (Ribobio, China).