ITS1 fungal amplicon of bovine: rumen liquid
Rumen samples were collected 3h after morning feeding using stomach tube. Samples were aliquoted snap frozen in dry ice and stored at -80 oC until DNA extraction. Total DNA was extracted as described by Rius et al. (2012). For fungal amplicon sequencing primers Neo 18S and Neo 5.8S from Edwards et al. (2008) were used.
FR4