GSM5145793: shCtrl-rep3; Homo sapiens; RNA-Seq
genome-wide CRISPR/Cas9 knockout library HepG2 cells are collected,and genome DNA was harvested and sgRNAs were amplified with PCR.  Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA library preparation is performed by using NEBNext UltraDirectional RNA Library Prep Kit for Illumina (E7420L).