RNA-seq of Oncopig Xenograft HCC Tumor
Total RNA was extracted from flash frozen samples using the AllPrep DNA/RNA Mini Kit (Qiagen) following the manufacturers protocol. RNA integrity and the presence of genomic DNA was tested on an Agilent 2100 Bioanalyzer using an RNA Nano bioanalyzer chip by the Carver High-Throughput DNA Sequencing and Genotyping Unit (HTS lab, University of Illinois, Urbana, IL, USA). Samples with RNA integrity numbers greater than 7 were utilized for sequencing. TruSeq stranded RNA-seq libraries (TruSeq Stranded RNA Sample Preparation Kit, Illumina) were generated from high-quality RNA by the HTS lab. The resulting RNA-seq libraries underwent Illumina sequencing on an Illumina HiSeq2000. The libraries were paired-end sequenced to a total read length of 100 base-pairs.
Tumor_44