GSM5217659: PA14_WT_400_2; Pseudomonas aeruginosa; RIP-Seq
After cell lysis by bead bashing, anti-FLAG magnetic beads were used to immunoprecipitate Hfq and its bound RNAs, RNA was then isolated by phenol-chloroform extraction. RNA libraries were prepared for sequencing using NEBNext Ultra II Directional RNA library kit