16S rRNA of Pacifigorgia cairnsi
DNAextractionwasmadeusingtheQuickDNAFecal/SoilMicrobeKits(ZymoResearch,USA)accordingtomanufactureinstructions.DNAfacility(IowaStateUniversity,IO,USA)preparedthelibrariesusingthehypervariableV4regionofthe16SrRNAgeneamplifiedwiththeprimers515Fand806R.Finalbarcodedampliconswerepooledandsequencedusing2x250pair-endtechnologyonanIlluminaMiSeqplatformatCenterforGenomeSciencesandSystemsBiology(WashingtonUniversity,StLouis,USA).
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