GSM6202149: Dnmt1_KO_3; Mus musculus; RNA-Seq
GSM6202149
10x Genomics Single Cell Gene Expression Single embryos were dissociated into single-cells using 200μl of TriplE Express for 10 minutes at 37°C on a shaking incubator then quenched with 1ml of ice-cold 10% FCS in PBS. Cells were filtered using a 40μM Flowmi cell strainer, span down at 300g for 5 minutes then resuspended in 50μl of PBS containing 0.04% BSA. Cells were counted and viability assessed using trypan blue staining on a Countess II instrument (Invitrogen). >95% of cells were negative for trypan blue indicating high sample quality. scRNA-seq was performed using 10x Genomics 3' v3 following manufacturers instructions and loading 16,000 cells. Sequencing was performed using an Illumina Novaseq using the recommended read lengths.