GSM6307603: C2; Homo sapiens; RNA-Seq
GSM6307603
Regions of interest in human grey matter were defined by immunostaining for hnRNP A1 to confirm grey matter regions with hnRNP A1 mislocalization in MS tissue, and grey matter regions with normal hnRNP A1 localization in control tissue, and the same region from an adjacent tissue section was dissected with a razor blade. RNA was extracted from each section (n=1 section per patient sample) using the Arcturus PicoPure extraction kit and its manufacturer's protocol. 10 ng of input RNA was used for library preparation using the Ovation SoLo RNA-Seq Library Preparation Kit (Tecan Genomics 0500-32) per the manufacturer's instructions, which included an rRNA depletion step using the AnyDeplete Human Probe Mix. Libraries were quantified using a Qubit 4.0 Fluorometer and Qubit 1X dsDNA HS assay before being run on a TapeStation 4150 to assess library quality. Barcoded libraries were pooled equimolar and run on a NextSeq550 generating 75-bp paired-end reads.