GSM5503164: AP7309; Mus musculus; RNA-Seq
Liver tissue was frozen in liquid nitrogen and stored at -80ºC. Small pieces of the frozen liver were mecanically fragmented with a tissue grinder and resuspended in 1mL of RNA solv-Reagent with 20 µL/mL of β-Mercaptoethanol. E.Z.N.A.® Total RNA Kit II (Omega bio-tek, Norcross, GA, USA) was used for total RNA isolation, following Manufacturer's protocol. The RNA-seq libraries were prepared with KAPA Stranded mRNA-Seq Illumina® Platforms Kit (Roche) following the manufacturer´s recommendations.