GSM5218862: M2; Mus musculus; RNA-Seq
Tumors were harvested 10 days after tumor challenge and CD45+ cells were sorted to be analyzed with 10x Twelve thousand tumor infiltrating immune cells were loaded for capture, using a Chromium Single Cell 3′ Reagent Kits v2 Chemistry (10× Genomics). Following capture and lysis, complementary DNA was synthesized and amplified (12 cycles) as per the manufacturer's protocol. The amplified cDNA was used to construct Illumina sequencing libraries and was sequenced on a HiSeq4000 system (Illumina). The Cell Ranger Single-Cell Software Suite v.3.0 (10× Genomics) was used to perform sample demultiplexing, barcode processing and single-cell 3′ counting.