Pool of single-cell RNA-seq of individual dissected ciliated male-specific cephalic sensory neurons, ventral left (CEM_VL)
Individual ciliated male-specific cephalic sensory (CEM) neurons were isolated from C. elegans expressing an integrated GFP labeling extracellular vesicle-releasing neurons (EVNs, ppkd-2::GFP [Figure 2A]), as previously described (Narayan et al., 2016; PubMed 26903633). Cells were separated by anatomical identity (i.e., CEM dorsal left [CEM_DL], CEM dorsal right [CEM_DR], CEM ventral left [CEM_VL], and CEM ventral right [CEM_VR]). Microdissection and single cell RT-PCR of individual CEM_DL, CEM_DR, CEM_VL, and CEM_DR neurons was performed essentially as described (Schwarz et al. 2012; PubMed 22991463). For all four neuronal types, single-end 50 nucleotide (nt) to 100-nt RNA-seq was performed on an Illumina HiSeq 2000.
CEM_VL_pool_15nov2021.R1.filt1