GSM4859720: OXO-PCR Patient 1031 sample 3E1; Homo sapiens; RNA-Seq
Frozen tumor samples were embedded in OCT (NEG-50, Richard-Allan Scientific) and 10 μm sections were taken using MB DynaSharp microtome blades (ThermoFisher Scientific) in a CryoStar cryostat microtome (ThermoFisher Scientific). Scrolls were also collected to be used for bulk RNA-seq. Bulk scrolls were immediately processed for RNA extraction using the RNeasy Mini kit (Qiagen). The extraction procedure was performed according to the manufacturer's instructions, including DNase digestion, after which RNA integrity was evaluated using the 2200 TapeStation (Agilent). The KAPA mRNA HyperPrep Kits (Roche) were use for the library preparation by following the manufacturer's instructions.