GSM6355462: ChIP-seq_PolII_S2P_mESC_NSD1-dTAG_dTAG-13_6h_batch1_bioRep1; Mus musculus; ChIP-Seq
GSM6355462
Cells were dissociated and crosslinked with 1% formaldehyde. Nuclei were isolated and chromatin was fragmented using a bioruptor sonicator. Drosophila chromatin was spiked in for Pol II ChIPs. Immunoprecipitation was performed using indicated antibodies and immunocomplexes were further pulled down using Protein G beads. Beads were washed and eluted followed by reverse crosslinking and DNA purification. Libraries were prepared with 3 ng ChIP or input DNA using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) according to manufacturer's protocol. Bead based sized selection was performed to enrich for DNA with ~250 bp inserts