GSM5149172: ESC O4f/- CreER 0h RNA rep2; Mus musculus; RNA-Seq
RNA was extracted using a Qiagen Rneasy mini kit with on-column DNA digest. Depletion of ribosomal RNA was performed on 2-5 μg of total RNA using the Ribo-Zero rRNA Removal Kitand libraries were produced from 10-100ng of ribosomal-depleted RNA using NextFlex Rapid Directional RNA-seq Kit