GSM4861383: human brain cell line 238; Homo sapiens; RNA-Seq
RNA was extracted from sub-confluent 10-cm plates using RNeasy mini kit (Qiagen, Cat No. 74104). RNA quality (RNA integrity number, RIN) and quantity was measured in a Bioanalyzer 2100 (Agilent) using the Agilent RNA 6000 Nano Kit (part number 5067-1511). The sequencing libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760S, New England Biolabs), with 200 ng of total RNA as input.