GSM6046518: R1-sc_SE_rep2; Zea mays; RNA-Seq
GSM6046518
Use Arcturus PicoPure RNA Isolation kit following the manufacturers instruction for LCM, and 5 ng of captured RNA was used for cDNA amplification by Ovation RNA-Seq System V2 kit (Nugen Technologies) following the manufacturer's protocols The construction of the cDNA paired-end libraries and their quality checking were carried out at the High-Throughput Genomics and Bioinformatic Analysis Shared Resource at HCI at the University of Utah, using a NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) with ~400 ng of amplified cDNA