GSM5502591: transfo_10d (scRNA-seq); Mus musculus; RNA-Seq
Cell suspension was mixed with master mix and loaded with Single Cell 3′ Gel Beads and Partitioning Oil into a Single Cell G Chip. RNA transcripts from single cells were uniquely barcoded and reverse-transcribed within droplets. cDNA molecules were pooled and the cDNA pool then go through an end repair process, the addition of a single 'A' base, and then ligation of the adapters. The products are then purified and enriched with PCR to create the final cDNA library. 10x Genomics Chromium 3' v3.1 Single Cell Gene Expression Kit protocol was followed according to manufacturer instructions. scRNAseq