Acinetobacter baumannii A118 exposed to cell-free conditioned media (CFCM) of S. aureus USA300. Replica 1.
Overnight cultures of A118 were diluted 1:10 in either fresh LB broth or in 100% CFCMUSA300 and incubated with agitation for 7 h at 37 C. RNA was immediately extracted following the TRI REAGENT Kit (Molecular Research Center, Inc., Cincinnati, Ohio, USA). Samples were confirmed to have no DNA contamination through PCR amplification of the 16S rDNA gene. RNA samples were subjected to ribosomal RNA-depletion using the Ribo-Zero kit (Illumina) and libraries constructed with the TruSeq Stranded Total RNA Library Prep kit (Illumina) from three independent replicates per sample.
A118+SAU1