WGS of E. coli: Lgt depletion revertant m3
DNA samples were fragmented by Covaris M220 Focused-Ultrasonicator (Covaris Ltd, Brighton, UK) using microTUBE-15 to 350 bp. The TruSeq DNA PCR-Free libraries prep kit (Illumina) was used following the instructions of the kit manufacturer. Sequencing was carried out on Illumina NextSeq 500 platform. Raw reads were processed with an in-house bioinformatics pipeline for quality
SLEC21m3