GSM6304507: siCtrl-METTL3_eCLIP-rep1; Homo sapiens; OTHER
GSM6304507
Total RNA was harvested using Trizol. polyA RNA was purified using magnetic-oligo(dT) beads. Chromatin fraction was used to extract caRNA. For meRIP-seq, m6A modified polyA or caRNA fragments were immunoprecipitated using anti-m6A antibody and then subjected to libraries construction SMARTER smRNA-Seq Library Prep Kit following manufacturer's protocols. For eCLIP-seq, experiments were performed following the ECODE eCLIP-seq protocol. meRIP-seq eCLIP-seq