ITS amplicon sequencing of Koko fermented millet Sunyani Sunyani
Fungal ITS amplification with primers ITS1F (5-CTTGGTCATTTAGAGGAAGTAA-3) (Jackson et al., 1999) and ITS2 (5-GCTGCGTTCTTCATCGATGC-3) (White et al., 1990) and the KAPA2G Robust HotStart PCR Kit (Sigma). PCR products purified using KAPA Pure Beads (Roche). Library prepared with the KAPA2G Robust HotStart PCR Kit and the Nextera XT Index Kit v2 index primers (Illumina).
ITS SUN.K