E. coli cells transformed with the DfrB1 DMS library were incubated with different concentrations of inducer (0.01%, 0.025%, 0.2%, 0.4% arabinose) and with 10 g/mL trimethoprim for 10 generations, with an intermediate dilution step at t = 5 generations. Sequenced at t = 0 and t = 10 generations. 3 biological replicates for each arabinose concentration.
E. coli cultures transformed with the DfrB1 DMS library were incubated with different concentrations of inducer (0.01%, 0.025%, 0.2%, 0.4% arabinose) for 10 generations. Sequenced at t= 0 and t= 10 generations. 3 biological replicates for each concentration
DfrB1-MiSeq