Determination of the complete mitochondrial genome of Holotrichia tilanis Reitter (ColeopteraScarabaeoidea)
the specimen of adult Holotrichia tilanis were obtained from Xining, Qinghai Province, China 3662N, 10177Ein May 2021 and stored in the Insect Collection of the Entomology Lab, College of Agriculture and Animal Husbandry, Qinghai University with Specimen Accession Number: ZYT-202105-01 (email:1282186715@qq.com). Genomic DNA was extracted from a single sample, then used Illumina NovaSe Sequencing Systemand (Illumina, San Diego, CA, USA) to determined the complete mitochondrial DNA sequence which read length was 150bp.The SPAdes v3.10.1 softwarehttp://cab.spbu.ru/software/spades/(Bankevich et al., 2012) was employed to assemble the mitogenome, gaps of which were filled with SSPACE 3.0 (Boetzer et al., 2011) and GapFiller 1.1 (Boetzer and Pirovano 2012). The assembled sequence was then annotated using the web server MITOS (Bernt et al., 2013). And then checked by manual proofreading.
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