GSM5070258: ChIPseq_FOXL2; Mus musculus; ChIP-Seq
Dissected ovaries from 8 weeks old female mice were snap-frozen and stored at −80◦C. Frozen ovaries were crushed in liquid nitrogen using a mini-liquid nitrogen cooled mortar (Bel-Art ref H37260-0100). Powdered tissue samples were immediately fixed in PBS/1% formaldehyde at room temperature for 20 min. Chromatin shearing was performed using qSonica Sonicator.  Chromatin immunoprecipitation (IP) with homemade rabbit anti TRIM28 and anti FOXL2 were performed on 20µg of chromatin using Low cell ChIPseq kit (Active motif, ref 10489). Each ChIPseq libraries were prepared from a pool of three independent IPs, each IP prepared with ovaries of two different animals. Next Gen DNA Library Kit which contains molecular identifiers (MIDs) used to remove PCR duplicates from sequencing data and Next Gen Indexing kit (Active motif, ref 104895).