GSM6139210: CRTL_2_3_2; Mus musculus; RNA-Seq
GSM6139210
6 kidneys for each genotype were harvested from 4 different pregnant females 48 hrs after initiating tamoxifen administration and dissociated into single cells. Five thousand FACS-sorted tdTomato+ cells were used for each biological replicate. The total RNAs were prepared from 5000 cells using a NucleoSpin RNA XS kit (Cat.740902, TakaraBio), and then mRNA was purified from total RNA using magnetic beads (S1550S, New England Biolabs). Random priming strand-specific RNA-seq libraries were prepared using the SMARTer stranded RNA-seq kit (Cat.634839, TakaraBio).