16S amplicon of poultry litter
Total DNA was extracted from 250 l of PL eluates saved in LB glycerol using the Qiagen DNeasy PowerLyzer Powersoil kit (Qiagen Inc. MD, USA) according to manufacturer instructions and published modifications (Bucher et al., 2020; Oladeinde et al., 2018). DNA extracted was used for preparing amplicon sequencing libraries as previously described (Allen et al., 2016). Briefly, the V1 -V3 hypervariable region of the bacterial 16S rRNA gene was PCR amplified and sequenced using the pairedend (250  2) method on the MiSeq platform
UC 12-42