NextSeq 500 paired end sequencing; RNA-seq for evaluation of the disruption of pluripotency and the related differentiation program in mouse embryonic stem cell (mESC) models
RNA-seq for evaluation of the disruption of pluripotency and the related differentiation program in mouse embryonic stem cell (mESC) models
03_d1_low_T_rep1_p
T(Brachyury)-inducible OCT4-YFP ESCs cultured in 2iLIF system were stimulated by doxycycline and dexamethasone for 24 hr in order to induce T. Different dose fractions of the T-induced cells were sorted and collected for the following RNA-seq experiment by using the fluorescence-Activated Cell Sorting (FACS) Analysis. Total RNA was isolated using a NucleoSpin RNA Mini kit (MACHEREY-NAGEL) and then, mRNA was isolated using NEB Next Poly A m-RNA magnetic bead selection kit (E7490S).  RNA-seq library was prepared using NEBNext Ultra II RNA Library Prep Kit for Illumina (E7770).