GSM5145809: penile corpus cavernosum-old_s1; Rattus norvegicus; RNA-Seq
For tissue dissociation, we continuously anesthetized all the rats with pentobarbital at one time and rapidly harvested their penile corpus cavernousm tissues (without tunica) with sterile instruments. Tissues were immediately minced to small fragments ~1 mm2, followed by enzymatic digestion with collagenase (~2h), elastase (~30min), and DNase (~1h) at 37 °C. Digestion was terminated by DMEM complete medium. The dissociated cells were centrifuged at 4 °C, filtered through a 70 μm filter, underwent red blood cell lysis and removal of dead cells using Dead Cell Removal Kit (Miltenyl Biotec). ScRNA-seq libraries were prepared  using standard Illumina protocols