GSM5219244: NGS192; Chlamydomonas reinhardtii; ncRNA-Seq
Mitoribosome purification was conducted as previously (Waltz et al., 2019, 2020). The RNA  was prepared  using TRI Reagent® (Molecular Research Center) according to the manufacturer's instructions. The RNA was not fragmented but enzymatically treated with Antarctic Phosphatase and T4 Polynucleotide Kinase. We used the Truseq Stranded Total RNA with Ribozero Plant, reference guide #10000000404499v00 October 2017 for the NGS192-total library preparation and the NEBNext multiplex small RNA Library set for Illumina reference E7580 for the NGS192-small library preparation.