GSM5411991: WM983B cell line in AM media, untreated, replicate1; Homo sapiens; RNA-Seq
Total RNA was isolated from cells using the Qiagen RNeasy® Mini Kit (Qiagen: 74106) according to the manufacturer's protocol. RNA quantity was determined using the Qubit 2.0 Fluorometer (ThermoFisher Scientific, Waltham, MA) and the quality was validated using the TapeStation RNA ScreenTape (Agilent, Santa Clara, CA). 500ng of DNAse I treated, total RNA was used to prepare library for Illumina Sequencing using the Quant-Seq 3'mRNA-Seq Library Preparation Kit (Lexogen, Vienna, Austria)