GSM5503498: PyMT_iEOS_2; Mus musculus; RNA-Seq
Eosinophils were directly sorted (>98% purity validated using Cytospin) into TRIzol (19367501, Ambion) supplemented with RNasin ribonuclease inhibitor (N2111, Promega), samples were then pooled from 3-4 mice. RNA was extracted by the addition of chloroform followed by centrifugation and addition of absolute ethanol to the top layer. Thereafter, the fluid was transferred to PureLink RNA mini kit (Invitrogen) and processed according to manufacturer's instructions.The RNA integrity number (RIN) was analyzed   using Bioanalyzer and only samples of RIN>6.3 were used. RNA libraries were prepared for sequencing using Cell-seq protocols