Illumina HiSeq 2500 sequencing; Cecal microbiota transfer rescues the antibiotic-induced acceleration of type 1 diabetes  and alteration of intestinal gene expression
Cecal microbiota transfer rescues the antibiotic-induced acceleration of type 1 diabetes  and alteration of intestinal gene expression
TP38_s
NOD mouse at P23, ileum tissue were collected and preserved in RNAlater and then frozen at -80C to extraction The dams and their litters were randomly assigned to control (C), 1PAT antibiotic (1P) and cecal microbiota transfer (CMT) groups. At postnatal (P) day 23, the pups were weaned and males were used for ileum sample collection.  All mice received acidified drinking water supplied by the facility routinely except for the periods when some litters were receiving antibiotic treatment: 1. Control (C): P5-P10 non-acidified drinking water.  2. antibiotic_1PAT treatment (1P): P5-P10 non acidified drinking water with 333mg/L antibiotic tylosin. 3. Cecal microbiota transfer treatment (CMT): P5-P10 non acidified drinking water with 333mg/L antibiotic tylosin. Then by oral gavage, 50, 75, and 100 uL of the suspension were transferred to 1P pups at P13, P15, and P17, respectively, representing the cecal microbiota transfer group (CMT).  TRIzol (Invitrogen) followed by RNeasy column cleanup (Qiagen) using the manufacturers protocols Ribosomal RNA was removed using the Ribo-Zero rRNA depletion kit (Illumina, San Diego CA). The TruSeq RNA sample preparation kit (Illumina, San Diego, CA) was used to prepare cDNA libraries from RNA samples.