GSM5218817: 97Q-Htt+RGFP109 cell 11; Mus musculus; RNA-Seq
Following electrophysiological recording at DIV15, single striatal GABAergic neurons or astrocytes were collected immediately by applying light negative pressure through the same glass patch pipette. Following successful aspiration, the sample was transferred in a volume of <2 μl of internal patch solution into a RNase-free PCR tube containing 1 μl Lysis buffer, supplemented with RNAse inhibitor, according to SMARTer Ultra Low RNA Kit (Clontech, Germany). Collected single cells were immediately spun down at room temperature and put on dry ice until -80°C storage. SMARTer cDNA synthesis was performed using SMARTer Ultra Low RNA Kit according to manufacturer's protocol. Single-cell cDNA library preparation was performed using Nextera XT DNA Library Prep Kit (Illumina, Germany).