RNA-seq of SLF infested 6-year-old hybrid Marquette
The ground tissue was homogenized in a cetyltrimethylammonium bromide (CTAB) based buffer with a chloroform denaturation step and the RNA was selectively precipitated with LiCl following Blanco-Ulate et al.. RNA was cleaned up using a RNeasy Plant Mini Kit (Qiagen Sciences Inc, Germantown, Maryland, USA) including the DNase treatment on column. Purity of extracted RNA was measured with a Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific, Waltham, Massachusetts, USA) and Bioanalyzer (Agilent 2100 Bioanalyzer system, Agilent Technologies, Santa Clara, California, USA).
SLF4