GSM5950013: p20191-s007_7_2019BC46_CD4_TOT; Homo sapiens; RNA-Seq
GSM5950013
RNA extraction was done with RNeasy Micro kit (Qiagen, 74004) with on-column DNase digestion. RNA quality was assessed using a Fragment Analyzer (Agilent) one nanogram of total RNA was used as input for cDNA synthesis using the Clontech SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio) according to the manufacturer's instructions. Amplified cDNA was fragmented and appended with dual-indexed bar codes using the Nextera XT DNA Library Preparation kit (Illumina, FC-131-1096). Libraries were validated by capillary electrophoresis on a Fragment Analyzer (Agilent), pooled at equimolar concentrations, and sequenced on an Illumina NovaSeq6000 at 100SR, yielding ~25 million reads per sample