GSM5693665: P7-lymph node-ST; Homo sapiens; RNA-Seq
For Visium, the section was cut at 10 µm thickness and mounted onto Visium slide capture areas. Sequencing libraries were prepared following the manufacturer's protocol. After tissue staining, bright-field images were taken as described in the Spatial Transcriptomics procedure. Tissue permeabilization was performed for 12 minutes, as established in the TO assay. The Visium Spatial Gene Expression Slide & Reagent kit (10X Genomics) was used to generate sequencing libraries for Visium samples. Visium spatial gene expression libraries were prepared for sequencing using 10x Genomics protocols.