GSM6046105: PBMC day 61 post-symptom onset; Homo sapiens; RNA-Seq
GSM6046105
Cells were washed in complete RPMI medium (supplemented with 10% FBS, 2mM L-glutamine, and 1% penicillin/streptomycin), counted, and assessed for viability using a Countess II automated cell counter (Invitrogen, Waltham, MA, USA). Cells were then suspended to 1 × 106/ml in complete RPMI for single-cell emulsion preparation. scRNA-seq GEX libraries were prepared according to 10X Genomics specifications. . Cell suspensions were loaded for droplet-encapsulation by the Chromium Controller (10X Genomics). Single-cell cDNA synthesis, amplification, and sequencing libraries were generated using the Single Cell 5′ Reagent kit (10X Genomics, Pleasanton, CA, USA) following the manufacturer's instructions. The libraries were sequenced with the Illumina NovaSeq 6000 platform (200 M, 150 bp paired-end reads).