Illumina NovaSeq 6000 paired end sequencing; RNA-seq of LSK isolated from U2af1 mutant, Tet2 mutant and U2af1/Tet2 double mutant against wild-type controls.
RNA-seq of LSK isolated from U2af1 mutant, Tet2 mutant and U2af1/Tet2 double mutant against wild-type controls.
M1_TET2_p
Bone marrow LSK cells were sorted from a pool of mice either WT, U2af1mut/+, Tet2-/- and U2af1mut/+ x Tet2-/-. Total RNA was extracted from LSK cells using the RNeasy Micro kit (Qiagen) following the manufacturerâ€™s instructions. On-column DNAse treatment was performed. Low input Directional RNA-seq library was prepared using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (Cat No. 7420).