GSM4983427: EV_1_1; Homo sapiens; RNA-Seq
RNA was extracted using TRIZOL reagent and treated with DNase I. mRNA was isolated using NEBNext Poly(A) mRNA isolation module (E7490, NEB) with using approximately 1µg total RNA. Then, RNA-Sequencing library was generated using NEBNext Ultra Directional RNA library Kit (E7420S, NEB) with isolated mRNA as input.