GSM5218553: NRH5045_aCSF_r2; Cryptococcus neoformans; RNA-Seq
These lyophilized samples were treated with Trizol and total RNA was extracted using the Qiagen RNeasy Mini Kit according to the manufacturer's instructions. Library preparation was prepared by shearing the RNA and adapted for Illumina sequencing using the TruSeq RNA Library Preparation Kit (Illumina, San Diego, CA) for YPD and CAP conditions or the Tag-Seq Protocol (Shishkin et al Nat Methods 2015) for the in vivo CSF and aCSF samples.