GSM5217228: HEK293T siTRMT61A RNA-seq rep1; Homo sapiens; RNA-Seq
Total RNAs was extracted from cell lines by TRIzol Reagent (Invitrogen #15596018) and Direct-zol RNA Miniprep Plus Kit with on-column DNase treatment (ZYMO #R2071). Total RNAs (0.1-1 μg) were poly-A selected by NEBNext poly(A) mRNA Magnetic Isolation Module (NEB #E7490) and followed by NEBNext UltraII Directional RNA Kit (NEB #7765).