RNA-seq of Homo sapiens: H9 ESCs induced NPCs
Total RNA was collected with the RNeasy mini kit from each sample (including one human fetal brain NSPCs sample, one human spinal cord NSPCs sample and one human H9 ESCs derived brain NSPCs sample) and stored at -80 C. RNA integrity was examined with the Agilent Bioanalyzer 2000 (Agilent, Santa Clara, CA). TrueSeq stranded mRNA-seq libraries were prepared from 1 g of total RNA (Illumina mRNA-seq kit, RS-122-2103) and sequenced on an Illumina NovaSeq platform.
H9-1