GSM5502221: 4008_Control_uninfected_2; Homo sapiens; RNA-Seq
Single cell suspensions were loaded onto a Chromium controller chip v3.1 (10X Genomics), with a target output of 6000-7000 cells per channel. GEM generation, barcoding, cDNA amplification and library preparation was performed using the Single-cell 3' Gel Bead and Library v3.1 kit (10X Genomics) at the Eukaryotic Single Cell Genomics facility (SciLifeLab, Sweden). Amplified cDNA and final libraries were evaluated on a Bioanalyzer for quality control and sequenced on Illumina NovaSeq 6000 platform. Single cell suspensions isolated from 3 organoids were pooled for each sample and loaded onto GEM wells. Each condition had 2 samples which were loaded onto individual GEM wells. Total number of GEM wells used were 6 (2 per experimental condition)