RNA-Seq:leaf
Total RNA was extracted from young leaves, female catkins, stems, and roots of FAFU-HL-1 using the Plant RNA Purification Reagent (Invitrogen) according to the manufacturers instructions. Genomic DNA was removed using DNase I (TaKara). An RNA-seq transcriptome library was prepared using the TruSeqTM RNA sample preparation Kit from Illumina (San Diego, CA) and sequencing was performed on an Illumina Novaseq 6000 by the Shanghai Majorbio Bio-pharm Biotechnology Co., Ltd., China (hereafter referred to as Majorbio).
FAFU-HL-1-L