GSM5218339: MCF10A_60kPa_rep1; Homo sapiens; RNA-Seq
Total RNA was isolated from biological samples with TRIzol (Invitrogen, 15596-018) according to the manufacturer's instructions. RNA-seq libraries (2 biological replicates per condition comprised of a pool of 4 PA-gel cultures each) were prepared from total RNA using KAPA mRNA HyperPrep Kit (Roche) and IDT dual indexed sequencing adaptors.