GSM5068329: ESC TIA1 rep2; Homo sapiens; RIP-Seq
Cells were washed twice in ice-cold PBS and lysed in RIP Lysis Buffer complemented with Protease and RNase Inhibitor Cocktails. Libraries were prepared using the Agilent Bravo automation system (Agilent Technologies, Santa Clara) using previously established protocol. Library preparation was done using the QuantSeq 3′ mRNA-Seq Library Prep Kit for Illumina (Lexogen, 015.24). The RNA was spiked with SIRV Set 3 Iso Mix E0/ERCC controls (Lexogen, 025.03) following manufacturer's instructions. Each sample was dual indexed during the final library amplification using the Lexogen's i5 6 nt Dual Indexing Add-on Kit (#047).