GSM5288903: Survivor 1 (Irradiated) (E2); Macaca mulatta; RNA-Seq
Total RNA was extracted from 40-80 mg frozen lung tissues from three different affected regions of the lung from eight irradiated animals (4 non-survivors and 4 survivors) and from two different randomly selected regions from three unirradiated control animals. Tissue was placed into a 1.4 mm ceramic bead tube with 1 ml QIAzol lysis reagent and homogenized using a bead mill (Bead Ruptor 24, Omni International, Kennesaw, GA). The tissue sample tube was processed on the Bead Ruptor for 1 cycle at a speed of 5.5 m/s for 20 sec; and repeated up to six times until the sample was completely homogenized. All homogenized lysates were extracted for total RNA using the RNeasy Microarray Tissue Mini kit (Qiagen, Germantown, MD). Extracted RNA was DNase-treated and purified using the RNA Clean and Concentrator-5 kit (Zymo Research, Irvine, CA), then assessed for RNA quality using an Agilent 2100 Bioanalyzer and the RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA) RNA libraries were prepared for sequencing using standard Illumina protocols