Complete chloroplast genome of Betula nigra
After extracting genomic DNA by CTAB method, the DNA was fragmented by mechanical interruption (ultrasonic), and then the fragment was purified, repaired at the end, A was added to the 3 'end, and the sequencing connector was connected. Then the fragment size was selected by agarose gel electrophoresis, and the sequencing library was amplified by PCR.
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