GSM5219243: p204; Chlamydomonas reinhardtii; ncRNA-Seq
Mitochondria purification was performed as in (Salinas et al. 2012) by digitonin treatment followed by a discontinuous Percoll gradient. The total RNA from mitochondria was prepared the RNA using TRI Reagent® (Molecular Research Center) according to the manufacturer's instructions. The RNA was first chemically fragmented (4 min) and then enzymatically treated with Antarctic Phosphatase (NEB#M0289S) and T4 Polynucleotide Kinase (NEB#M0201S). Library preparation was done according to the TruSeq Small RNA Sample Preparation Guide #15004197 Rev. F February 2014.