Metagenome of soil and root-associated microorganisms
To demultiplex the data, we used Qiime's split_libraries_fastq.py command and relevant barcode and metadata mapping files. To get access to these files, please email leeconar@iastate.edu or larryh@iastate.edu. The demultiplex code is as follows: split_libraries_fastq.py -i $PWD/fastq-join_joined_CutaQ25/fastqjoin.join.fastq -b $PWD/fastq-join_joined_CutaQ25/fastqjoin.join_barcodes.fastq --rev_comp_mapping_barcodes -o Cuta_UPARSEQ25/ -m Chen_map.txt -r 1 --store_demultiplexed_fastq --store_qual_scores -q 0 --barcode_type 16
16S_rhizotron_multiplex