GSM6201361: Fluconazole treated-Day2-replicate2 [FCZ_2_2 ]; Candida albicans SC5314; RNA-Seq
GSM6201361
Cells were suspended in RNAlater and stored in -20 for 2 to 3 days. After Zymolyase digestion to make spheroplasts, the transcriptomes of single cells were captured by polyT oligonucleotides attached to the surface of  specific microparticles(Chemegenes beads) in a microfluidic device. cDNAs were made and amplified for sequencing as described in Macosko et al 2015. 600pg cDNA were used for making sequencing libraries. Sequencing libraries were prepared using Illumina protocol and samples were sequenced with Nextseq500-- 0.5 Flowcell High Output (200 M  fragments)-- 75 cycles Single-End read (maximum 1x85 nt)