ciliate protozoa 18S rRNA gene amplicon of bovine: rumen liquid
Rumen samples were collected 3h after morning feeding using stomach tube. Samples were aliquoted snap frozen in dry ice and stored at -80 oC until DNA extraction. Total DNA was extracted as described by Rius et al. (2012). For ciliate protozoa amplicon sequencing universal primers targeting 18S rRNA gene were used (Sylvester et al., 2004).
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