Bacillus
DNA was isolated from bacterial biomass using DNA Extraction Kit (BIO-RAD). The purity of the isolated DNA was assessed by agarose gel electrophoresis, and the DNA concentration was determined using the Qubit dsDNA BR assay kit and Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA). The 16S rRNA gene was amplified by PCR with primers and pyrosequenced using the GS Junior System (454 Life Sciences, Roche).
Bacillus