GSM5288876: Pancreatic islets cells db/db Sham sample 1; Mus musculus; RNA-Seq
Islets were freshly isolated using collagenase and subsequent gradient centrifugation. Islets were dispersed into single-cells using TrypLE. Single cell libraries were generated using the ChromiumTM Single cell 3' GEM, library and gel bead kit v3 (#1000075) from 10x Genomics. Libraries were sequenced on the NovaSeq6000 (Illumina) with 150bp paired-end sequencing.