GSM5693853: PDAC Cancer Replicate1; Mus musculus; RNA-Seq
Mice were sacrificed and pancreata samples were harvested for RNA-Seq, following the final caerulein injection. Total RNA was extracted from tissue samples using Qiagen RNeasy Plus Universal mini kit following manufacturer's instructions (Qiagen, Hilden, Germany). Extracted RNA samples were quantified using Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA, USA) and RNA integrity was checked using Agilent TapeStation 4200 (Agilent Technologies, Palo Alto, CA, USA). RNA libraries were prepared for sequencing using standard Illumina protocols