RNA-Seq or Ephydatia muelleri primmorph treated with MRTF activator ISX9
Juvenile Ephydatia muelleri were lab grown from cleaned gemmules for 3 days. Sponges were then dissociated into individual cells and archeocytes (stem cells) were enriched for by centrifugation. Cells were then resuspended in the MRTF activating drug (ISX9) or vehicle control and allowed to form primmorphs in an attachment free environment for 3 days. After 3 days, total RNA was extracted from primmorphs, quality controlled, and mRNA was enriched by oligo(dT) beads. mRNA was randomly fractured and synthesized into cDNA with random hexamer primers. Sequencing and indexing adapters were ligated to the cDNA and samples were fed into Illumina sequencers. Reads were then filtered on quality scoring and adapter sequences were removed.
Emue_ISX_Primmorph_r1