GSM5746576: Fem_AI_d32_6R1; Bos taurus; RNA-Seq
Placentas were collected, flash frozen on liquid nitrogen, and RNA was harvested using ALLprep or Trizol reagent. poly-A containing mRNA is purified from total RNA(1 ug), RNA is fragmented, double-stranded cDNA is generated from fragmented RNA, and the index containing adapters are ligated to the ends.  The amplified cDNA constructs were purified by addition of Axyprep Mag PCR Clean-up beads.  The final construct of each purified library was evaluated using the Fragment Analyzer automated electrophoresis system, quantified with the Qubit flourometer using the Qubit HS dsDNA assay kit, and diluted according to Illumina's standard sequencing protocol for sequencing on the NovaSeq 6000