RNA-Seq; Toxoplasma gondii; SR1KO_R3
Parasite RNA was extracted using TRI Reagent (Sigma) followed by the RNeasy MinElute Cleanup Kit (Qiagen) according to the manufacturers protocol. DNase 1 (Qiagen) was used to remove contaminating DNA followed by a second cleanup. Library preparation and sequencing was performed by Victorian Clinical Genetics Services using TruSeq stranded mRNA kit (Illumina) and NovaSeq 6000 to a depth of 30M reads
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