GSM6304516: Ear Tag: 4, Cre+ side, Tx: RT; Mus musculus; RNA-Seq
GSM6304516
Total RNA was extracted from frozen tissue using TRIzol (Invitrogen) and RNeasy Mini kits (Qiagen). Total RNA samples were prepared into RNAseq libraries using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® following the manufacturer's recommended protocol. Briefly, 1ug total RNA was polyA selected for each sample, converted to double-stranded cDNA followed by fragmentation and ligation of sequencing adapters. The library was then PCR amplified for 8 cycles using barcoded PCR primers, purified, and size selected using AMPure XP Beads before loading onto an Illumina NextSeq2000 for 100 base single read sequencing.