GSM6304175: Patient2 esophageal cancer tissue; Homo sapiens; ncRNA-Seq
GSM6304175
tRNA derived fragments (tRF & tiRNA) are heavily decorated by RNA modifications that interfere with small RNA-seq library construction. We do the following treatments before library preparation for total RNA samples: 3'-aminoacyl (charged) deacylation to 3'-OH for 3'adaptor ligation, 3'-cP (2',3'-cyclic phosphate) removal to 3'-OH for 3'adaptor ligation, 5'-OH (hydroxyl group) phosphorylation to 5'-P for 5'-adaptor ligation, m1A and m3C demethylation for efficient reverse transcription. Sequencing libraries are size-selected for the RNA biotypes to be sequenced using an automated gel cutter. The libraries are qualified and absolutely quantified using Agilent BioAnalyzer 2101. For standard small RNA sequencing on Illumina NextSeq instrument, the sequencing type is 50bp singleread.