GSM5453782: hESC differentiated towards interneurons using lenti Ascl1 and Dlx2, replicate 1; Homo sapiens; RNA-Seq
Cultures were dissociated in Trypsin and passed through 100uM cell strainers in preparation for single cell sequencing. Single cell suspensions were co-flowed with poly-dT beads, and then cDNA was formed following the standard DropSeq protocol using RT, Kapa polymerase, and Nextera XT transposase. Libraries were size selected against adapters by gel extraction above 200bp. Libraries were sequenced at 25bp through the Read1 barcode and 75bp through the Read2 genomic sequence.