16S amplicon seq of Citrus sinensis: leaves and roots across phenological stages
Amplified the bacterial rRNA 16S V4 region using the 515FB/806RB primer set and the standardized Earth Microbiome Project protocol, which can be found online at http://press.igsb.anl.gov/earthmicrobiome/protocols-and-standards/16s/ (Gilbert, 2012). To limit the amplification of plant mitochondrial and plastid 16S regions, we used pPNA and mPNA clamps
L8R1