RNAseq of Dahlia: senescent florets
For RNA sequencing three stages of cv. Sylvia florets were used: inner stage III florets (SIII-in) and both inner and outer stage IV florets (SIV-in, and SIV-out) (Supplementary Fig. S1). Three biological replicates for each developmental stage were obtained by mixing nine florets from the same stage of three flower heads and dividing into three random groups. Florets for each replicate were ground under liquid nitrogen and RNA extracted using an RNEasy Plant MiniKit (QIAGEN). All RNA samples were quality tested using a Qubit fluorometer and then sequenced using an Illumina NextSeq 500 to produce paired-end reads for each sample.
Matt2_S5