GSM6046137: MCF7, ChIP, ERa, siCtl, E2; Homo sapiens; ChIP-Seq
GSM6046137
For ChIP ~2-4 X106 MCF7 cells treated with E2 for the indicated times were fixed with ChIP Cross-link Gold (Diagenode) according to the manufacturer's instructions. Nuclei were purified and fragmented using a nuclear Co-IP kit (Active Motif). After digestion, chromatin was sonicated to an average size of 250 bp. The fragmented chromatin was immunoprecipitated overnight by using the corresponding antibodies. For RNA-seq, total RNA was extracted with Trizol following manufacturer's instructions For ChIP-seq, the NEBNext Ultra II DNA Library Prep Kit was used to produce high yield libraries from a small amount of purified DNA. For RNA-seq, the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina was used, using 1ug of total RNA as Input