GSM5949987: Retinoid-containing lung cells_WI02; Mus musculus; RNA-Seq
GSM5949987
Mouse lung digests for single-cell flow cytometry were prepared from wild type C57BL/6 mice using a method involving digestion with pronase and collagenase. Flow cytometry was employed to sort live, individual retinoid-containing cells based on their emission at 455 nm (UV-positive cells).  Singlet discrimination was sequentially performed using plots for forward scatter (FSC-A versus FSC-H) and side scatter (SSC-W versus SSC-H). Dead cells were excluded by scatter characteristics and staining with propidium iodide.  All FACS isolations were performed on a FACSAria cell sorter (Becton Dickinson). Single cell libraries were prepared using the Chromium Single Cell 3' Reagent Kits v3.1 (10x Genomics) according to the manufacturer's instructions followed by their sequencing on a NovaSeq 6000 (Illumina) in a 2 x 100 bp configuration