Illumina HiSeq 4000 sequencing
Targeted control dataset for SNP discovery
34 Control
DNA was restriction digested using the methyl-insensitive restriction enzyme Mspl, which cuts the DNA at CCGG sites. The fragments were blunt-ended and phosphorylated, and a single A nucleotide was added to the 3' ends of the fragments in preparation for ligation to a methylated adapter with a single-base T overhang. The ligation products were purified and size-selected (30 - 160 bp) using agarose gel electrophoresis. The treated DNA was PCR-amplified to enrich for fragments with adapters on both ends.