GSM4859912: Dis3l2 P4 cKO2; Mus musculus; RNA-Seq
Testess were isolated, total RNA was isolated using a RNeasy Micro Kit. The libraries were constructed by using a Universal RNA-Seq Library Preparation Kit (TECAN) per the manufacturer's protocol in which double-strand cDNA was generated using a mixture of random and poly(T) priming, followed by fragmentation of double-stranded cDNA, end repair, adaptor ligation, strand selection, targeted transcript depletion with AnyDeplete, and PCR amplification to produce the final library.