Mitochondrial DNA sequencing of homo sapiens: Cancer stem-like cells derived from SAS cell line (Native)
Mitochondrial DNA (mtDNA) was extracted using a QIAprep Miniprep Kit (QIAGEN, Germany). The mtDNA fraction was enriched and size-selected using solid-phase reversible immobilization (SPRI) paramagnetic beads (Agencourt AMPure XP; Beckman Coulter Inc., USA). The DNA samples were treated with BamHI (Vivantis Technologies, Malaysia) to linearise mtDNA. The DNA libraries were prepared using the 1D Ligation Sequencing Kit (SQK-LSK108; Oxford Nanopore Technologies, UK).
SAS_Tumour_Spheres_Native