GSM4983836: HL60 EIF2S3, DOX(-); Homo sapiens; RNA-Seq
RNA was harvested using Trizol reagent.RNA degradation and contamination were monitored on 1% agarose gels and purity was checked by the NanoPhotometer spectrophotometer. RNA concentration was measured using Qubit RNA Assay Kit in Qubit 2.0 Flurometer and integrity was assessed using the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzeer 2100 system. Sequencing libraries were generated using NEBNest UltraTM RNA Library Prep Kit for IlluminaR (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.