GSM5217496: Withdrawal from chronic ethanol liquid diet rep4; Rattus norvegicus; RNA-Seq
Brains were collected for the dissection of Hippocampus. Followed by total RNA extraction using the miRNEasy protocol, which was used for RNA-sequencing (paired-end) using the Illumina platform. cDNA fragments were 80bp to 500bp, with an average length of 250bp. RNA integrity numbers (RIN) for each RNA sample were determined using an Agilent Tapestation and were all above 8.0. Illumina True-Seq Stranded libraries were prepared and quantitated by qPCR. Reads were 100nt in length. The RNAseq libraries were prepared with Illumina's 'TruSeq Stranded mRNAseq Sample Prep kit' (Illumina). Read 1 aligns to the ANTISENSE strand, whereas Read 2 aligns to the SENSE strand.  The libraries were quantitated by qPCR and sequenced on 11 lanes (8 lanes on one flowcell, 3 lanes on another flowcell) for 101 cycles from each end of the fragments on a HiSeq 4000 using a HiSeq 4000 sequencing kit version 1.  Adaptors have been trimmed from the 3'-end of the reads.