WGS of 15 divergently selected maize lines for stalk strength
Individual leaf samples from 96 plants of each of the three populations (C0, C15-L, and C15-H) were collected from field-grown plants at the V8 stage, flash-frozen in liquid nitrogen, and stored at -80C. DNA of each plant was extracted as published (Saghai-Maroof et al. 1984), cleaned using the Qiagen DNeasy 96 Plant kit (Qiagen, Germantown, MD), diluted to 20ng/l, and approximately 200ng DNA was used for modified ApeKI genotyping-by-sequencing (GBS) (Elshire et al. 2011). The modification included separating each 96 well plate into 4 pools of 24 adapter-ligated samples. All 288 individually barcoded samples were pooled and single-end 75-bp sequencing was performed on NextSeq High Output sequencer at University of Missouri, Columbia.
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