GSM6304327: FVB, 10 months, mouse 4; Mus musculus; RNA-Seq
GSM6304327
Apexes of LV were dissected from hearts and homognized in TRIzol reagent. Total RNA was extracted from cardiac tissues (i.e., apex of the heart) using an RNeasy Mini Kit (Qiagen) with DNAse treatment. 1 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared with Sense mRNA-Seq Library prep Kit v2 (Lexogen) according to the manufacturer's protocol. Five biological replicates in each group for a total of 50 samples (5 replicates*(5 time points)*(2 strains)) were submitted for RNA sequencing.