RNA-seq of hard clam haemocyte  after air exposure for 5 days
In order to keep the conditions of air exposure unchanged, the hard clams were put into a multi-thermo incubator  with temperature and humidity set at 15  and 60%, respectively. After exposure for 5 days, 18 live clams were randomly sampled and Hemolymphs from six clams were pooled into one sample and three were three replicates. The hemolymph was filtered through a 70 m cell strainer to eliminate debris and haemocytes were obtained by centrifugation of whole hemolymph at 800 g, 4 for 8 min.
PostAE5