PDLSCs under 0.1 Hz: group 1
Total RNA for each group was extracted by using Trizol reagent kit (Invitrogen, USA). The mRNA were enriched and then fractured into short pieces prior to the synthesis and purification of double stranded cDNA, then the fragments were ligated to Illumina sequencing adaptors. Low quality sequences were removed from the raw reads before the sample library products were sequenced using an Illumina NovaSeq (Gene Denovo, China).
0.1_1