Throat swab of ferret F1124 (donor ferret) collected 3 days post inoculation
86 amplicons of 500bp with 75bp overlap between the different amplicons were amplified by RT-qPCR. Amplicons were purifiedwith 0.8x AMPure XP beads (Beckman Coulter) and 100ng of DNA wasconverted into paired-end Illuminasequencing libraries using KAPA HyperPlus library preparation kit (Roche), following the manufacturer's recommendations, to enable subsequent sequencing of multiple libraries in a singleIlluminaV3 MiSeq flowcell (2x300 cycles).
F1124_TS_3dpi