GSM4983904: Mx1-Jak2VF-WT 4; Mus musculus; RNA-Seq
Spleens were harvested from mice then passed through a 0.45um cell filter, washed and incubated with CD11b beads. CD11b positive cells were isoalted using a magnetic field, then washed and collected fro RNA isolation. RNA with RIN > 8 was subjected to poly-dT pulldown using magnetic beads (NEB) before preparation for RNA-seq using RNA Ultra kits (NEB).