GSM6202819: A2780S; Homo sapiens; miRNA-Seq
GSM6202819
RNA was extracted using miRCURY RNA Isolation Kit - Cell and Plant (Exiqon, Denmark) Total RNA of each sample was sequentially ligated to 3' and 5' small RNA adapters. cDNA was then synthesized and amplified using Illumina's proprietary RT primers and amplification primers. Subsequently, ~130-150 bp PCR amplified fragments were extracted and purified from the PAGE gel. Sequencing was performed on Illumina HiSeq 2000 using Truseq Rapid SBS Kits, according to the manufacturer's instructions.