Whole Genome Sequencing of Skull-Based Chordoma: Sample T2027
"First, we removed ribosomal RNA using the Epicentre Ribo-zeroTM rRNA Removal Kit (Epicentre, USA). Subsequently, sequencing libraries were generated using the rRNA-depleted RNA and NEBNext UltraTM Directional RNA Library Prep Kit from Illumina (NEB, USA) following manufacturer's recommendations. After adapter ligation and library amplification, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA) in order to select fragments of preferentially 150~200 bp in length. The strand marked with dUTP was not amplified, allowing strand-specific sequencing. Finally, products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system."
T2027