sRNA-Seq of mesocosm
To eliminate RNA molecules that are not packed into vesicles, we subjected vesicle samples to RNase treatment prior to RNA extraction. Total  RNA from vesicles was extracted using the miRNeasy kit  (Qiagen). Libraries were prepared using the TruSeq Small RNA Library kit (Illumina). After 15 cycles of PCR amplification, libraries were cleaned with the QIAquick PCR Purification Kit (Qiagen). All the kits were used according to the manufacturers instructions.
Mes-Ves_3