RNA seq of activated WT CD4 T cell
RNA-seq libraries were generated using TruSeq Stranded Total RNA with Ribo-Zero Globin Complete kit (Illumina, CA). Ribosomal RNA (rRNA) was removed using target-specific oligos combined with rRNA removal beads. Following rRNA removal, mRNA was fragmented and converted into ds cDNA,million paired-end 150bp reads were generated per sample using Illumina HiSeq 4000 platform.
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