GSM5289433: CC003_rep1; Mus musculus; ATAC-seq
Airway macrophage pellets were gently lysed to release nuclei, which were pelleted by centrifugation and used directly for transposition reaction. PCR amplification of the transposed DNA fragments was performed to add custom indexing primers. Final libraries were purified by double-sided size selection using Agencourt AMPure XP magnetic beads.