Illumina NovaSeq 6000 sequencing; RNA sequencing of RipmiR-141~200c and RipmiR-141~200c Zeb1200M islets
RNA sequencing of RipmiR-141~200c and RipmiR-141~200c Zeb1200M islets
RipmiR-141~200c Zeb1200M 3_s
Islets were isolated from mice by perfusing Liberase enzyme into the common bile duct. RNA was extracted using Trizol, then purified using Picopure columns with on-column DNase treatment.  Library construction was performed using SmartSeq II. For SmartSeq II we use a homemade recipe published in the Picelli Paper (cDNA): https://www.nature.com/articles/nprot.2014.006. For the sequencing library we have used the Nextera kit you finder: https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/nextera-xt-dna.html