GSM5502238: Embryonic Salivary Gland scRNA Sequencing; Mus musculus; RNA-Seq
Capture 12,359 cells using the 10x Chromium System done by Singulomics Libraries were prepared according to 10x Genomics Chrmoium Next GEM Single Cell 3' GEM Kit instructions (Cat# 1000130). Briefly, single cells were emulsified in 10X Chromium system and each cell's RNA was barcoded. cDNA was amplified, fragmented and indexed primers 10-13 cycles and library fragments of ~400 bp. The cDNA was flowed onto a Illumina flow cell for cluster generation. Libraries were sequenced on 1 lane of a Illumina HiSeq for 400 million PE150 reads following the manufacturer's protocols. scRNA-Seq / Drop-Seq