GSM6139200: Tyk2fl3; Mus musculus; RNA-Seq
GSM6139200
Colonic tumors were induced using AOM/DSS. Colons were disected, flushed with PBS and cut open to reveil the colon lumen. Single tumors were removed using disecting tools and homogenized in Trizol. RNA purification was performed using a Quiagen RNeasy Kit ( Cat# 75144). RNAseq libraries were constructed using 1ug of total RNA. RNA libraries were prepared for sequencing using standard Illumina protocols