uncertified S. tuberosum tuber-seeds from northern Antioquia
Total RNA was extracted from 100 mg of the top two centimeters at the tip of each individual tuber sprout. RNA was purified using the GeneJET Plant RNA Purification Mini Kit (Thermo Fisher Scientific, EEUU). cDNA libraries for HTS were prepared with the TruSeq Stranded Total RNA LT Sample Prep kit using bulked total RNA from certified (BST19) and uncertified (BST20) S. tuberosum cv. Diacol Capiro seeds; and uncertified (BSP18) S. phureja cv. Criolla Colombia. Ribosomal RNA (rRNA) was removed with the Ribo-Zero Plant kit (Illumina, USA).
BST20