NextSeq 500 paired end sequencing; CUT&RUN Targeting SALL4 and HDAC2 in human melanoma cells
CUT&RUN Targeting SALL4 and HDAC2 in human melanoma cells
HDAC2_1_p
500 000 human melanoma cells (M010817) were harvested for CUT&RUN. Antibodies were incubated overnight at a 1:100 dilution. CUTANA pAG-MNase was used at 0.5X concentration and digestion was performed for 30 minutes. Phenol:Chloroform KAPA HyperPrep Kit with KAPA DUI Adapters