GSM5150269: shNC2; Sus scrofa; RNA-Seq
1.Total RNA was extracted using RNAiso Plus reagent; 2.mRNA enrichment and purification: Oligo dT Selection to enrich the mRNA; 3.RNA fragmentation and cDNA synthesis; 4.End repair, add A and adaptor ligation; 5.PCR; 6.Circularization and make DNB. RNA libraries were prepared for sequencing using standard BGISEQ-500 protocols