GSM5068537: Control_8w_ATACseq_rep2; Mus musculus; ATAC-seq
Dentate gyrus were dissected from control or MDZ-exposed Nestin-EGFP mice at P10 and 8w and then EGFP-positive cells were isolated by FACS. Approximately 100,000 (P10) and  30,000- 50,000 (8w) cells were lyzed in lysis buffer.  After centrifugation, nuclei were resuspend in reaction buffer containing Tn5 transposase. Tagmented DNA was used for library amplification. Libraries were prepared for sequencing following standard Illumina protocols