GSM5746140: ChIP-Seq BatfKO_input_S18; Mus musculus; ChIP-Seq
Briefly, cells were fixed with 1% formaldehyde and genomic DNA was fragmented by Covaris sonicator (E220). The fragmented DNA was then subjected to immunoprecipitation with transcription factor-specific antibodies. Briefly, the NEBNext ChIP-seq Library Prep Kit (NEB #E6240L) was used to end-repair and adding adaptor to the immunoprecipitated DNA fragments according to the manufacurer's protocol. NEBNext Multiplex Oligos for Illumina Index Primers Sets were then used to PCR amplify and barcode the libraries.