Hi-C of Alosa alosa
Hi-C library was prepared according to a protocol adapted from Rao et al. 2014 (Foissac et al. (2018); https://doi.org/10.1101/316091).  Blood sample was spin down, and cell pellet was resuspended and fixed in 1% formaldehyde. 5 million cells were processed for the Hi-C library. After overnight digestion with HindIII (NEB), DNA ends were labeled with Biotin-14-DCTP (Invitrogen) using the klenow (NEB) and religated. 1.4 g of DNA was sheared to an average size of 550 bp (Covaris). Biotinylated DNA fragments were pulled down using M280 Streptavidin Dynabeads (Invitrogen) and ligated to PE adaptors (Illumina). The Hi-C library were amplified using PE primers (Illumina) with 10 PCR amplification cycles. The library was sequenced on an Illumina NovaSeq6000 platform to generate 128 million 2 x150 bp read pairs.
Aalosa_HiC