GSM5746860: CTCF(Δ5|6:6|7) MN dual positive rep2; Mus musculus; OTHER
Cells were harvested, and genomic DNA was isolated as described before (Sanjana et al., 2014 and Shalem et al., 2014). CRISPR libraries were prepared at each time point, and/or sorted population according to previously described protocol mainly consisting of two-step PCRs (Sanjana et al., 2014 and Shalem et al., 2014) CRISPR knock-out