GSM6139220: mouse tissue, HGG Sox9-GOF, rep 2; Mus musculus; RNA-Seq
GSM6139220
Tumor tissue was collected and frozen in trizol. After thaw, tissues were dissociated and total RNA was isolated using trizol and chloroform, and purified using the RNeasy Mini Kit (QIAGEN). Illumina sequencing libraries with 6-bp single indices were constructed from 300 ng total RNA using the TruSeq Stranded mRNA LT kit (Illumina, RS-122-2101).