GSM4861683: Early_control, rep1; Saccharomyces cerevisiae; RNA-Seq
The total RNA was extracted using TRIzol Reagent according to the manufacturer's instructions (Invitrogen) and genomic DNA was removed using DNase I (TaKara, China). RNA-seq transcriptome library was prepared using TruSeq RNA sample preparation Kit from Illumina (San Diego, CA). Libraries were size selected for cDNA target fragments of 200–300 bp on 2% Low Range Ultra Agarose followed by PCR amplified using Phusion DNA polymerase (NEB) for 15 PCR cycles. After quantified by TBS380, paired-end RNA-seq sequencing library was sequenced with the Illumina NovaSeq 6000 sequencer. The raw paired end reads were trimmed and quality controlled by SeqPrep and Sickle software with default parameters. Then clean reads were separately aligned to reference genome with orientation mode using TopHat software.