GSM5289072: Ym1_positive_monocytes_rep1; Mus musculus; RNA-Seq
Lung monocytes were removed by cell sorter and RNA was harvested using RNeasy Mini kit (QIAGEN). SMART-Seq v4 Ultra Low Input RNA Kit (TAKARA) and Nextera XT DNA Library Preparation Kit (Illumina) was used with 500 upg of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing.