UCE target enrichment of Anas-platyrhynchos-DDG1776: muscle tissue
Our laboratory procedures followed Glenn et al. (2019). In short, we prepared dual-indexed DNA libraries which were quantified using a Qubit fluorimeter (Invitrogen, Inc., Carlsbad, CA, USA). We then enriched the samples with 5,060 UCE loci using the Tetrapods-UCE-5Kv1 kit from MYcroarray following version 1.5 of the UCE enrichment protocol and version 2.4 of the post-enrichment amplification protocol (http://ultraconserved.org). The resulting pool was then sequenced using a paired-end 150 (PE150) protocol on an Illumina HiSeq 2500 using three lanes (Illumina, Inc., San Diego, CA, USA; UCLA Neuroscience Genomics Core).
Anas-platyrhynchos-DDG1776