RNA-Seq of Arum maculatum (Araceae): Appendix tissue sampled during anthesis
Approximately 100 mg of tissue was rinsed in sterile RNase-free water, flash frozen in liquid nitrogen, powdered in a QIAGEN TissueLyser II, and immediately extracted using a QIAGEN RNeasy Plant Mini Kit, following the manufacturers protocol. Extraction quality and concentration were verified using an Agilent Fragment Analyzer. Illumina TruSeq Stranded mRNA polyA libraries were prepared from these samples, following the manufacturers recommended protocol.
VIS001APP