16S rRNA gene samples collected in control sample 3 in February
Microbial genomic DNA was extracted from the gut contents samples using the PowerFecal DNA Isolation Kit (Mo Bio, CA, USA) following the manufacturers instructions. The V4-V5 hypervariable region of the bacterial 16S rRNA gene was amplied with a forward primer 515F and a reverse primer 909R containing a unique 14-base barcode.
CGM3