GSM6046575: MC38ishTDO2-48h-2; Mus musculus; RNA-Seq
GSM6046575
Cells were pelleted and RNA was isolated with RNeasy Mini Kit (Qiagen, Cat# 74104). RNA was reverse-transcribed into cDNA following SuperScriptTM III First-Strand Synthesis SuperMix (Invitrogen, Cat# 18080400). qRT-PCR was performed using SYBR Green PCR Master Mix (Thermo Fisher Scientific) in a 7500 Fast Real-Time PCR instrument (Applied Biosystems). For RNA sequencing, RNAs were isolated from ishTDO2 APC-WT and APC-KO MC38 cells with and without Dox treatment (biological triplicates per group) using the RNeasy Mini Kit.