RNA-Seq of Digitonthophagus gazella: male foretibia
We collected fore- and hindleg tissue for six males reared under high food conditions, foreleg tissue of six males reared under poor food conditions, and foreleg tissue of six females reared under high resource availability (24 samples of 18 individuals total). Tissue was stored immediately in RNAlater and kept at -80C. After thawing, tissues were homogenized with disposable polypropylene RNase-free pestles and RNA was extracted using a RNeasy Plus Mini Kit (Qiagen) following the manufacturers instructions. Total RNA quality was checked using an RNA ScreenTape TapeStation System (Agilent). Libraries were quantified using a Quant-iT DNA Assay Kit (Thermo Fisher), pooled in equal molar amounts, and sequenced as single-end reads using a 75-cycles High kit on the NextSeq500 platform (Illumina, San Diego, CA). Samples were all pooled in each lane of sequencing to control for batch effects.
L_rep6