Illumina HiSeq 2500 sequencing; High throughput sequencing of polyA-RNAs in HIV/HCV coinfected patients before and after treatment with with direct-acting antivirals
High throughput sequencing of polyA-RNAs in HIV/HCV coinfected patients before and after treatment with with direct-acting antivirals
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Peripheral venous blood samples were collected in EDTA tubes. Total RNA was extracted from Peripheral Blood Mononuclear Cells with the RNeasy minikit (Qiagen™), according to the manufacturer's instructions.  Libraries were synthesize with the Illumina's TruSeq Stranded mRNA Sample Prep Kit v2 using 500 nanogram of total RNA following manufacturer's protocol.