Illumina sequencing of Arima HiC library of Bombus affinis adult male
Tissue was crosslinked using the Arima HiC low input protocol, and proximity ligation was performed using Arimas HiC Kit. After proximity ligation, the DNA was sheared using a Diagenode Bioruptor and then size-selected to enrich for DNA fragments of 200-600bp. An Illumina library was prepared from the sheared and size-selected DNA using the Swift Accel NGS 2S Plus kit. The final Illumina HiC library was sequenced on a NovaSeq 6000.
iyBomAffi1_Illumina_HiC