RNAseq of human PBMCs: male wih a new tuberculosis infection
Total RNA extraction was done from the PBMCs using the commercial kit RNeasy Plus Mini Kit (Qiagen).Samples with an RNA Integrity Number (RIN) 7, ratio of 260/280 1.8 and 28S/18S ratio 1.5 were selected for sequencing.The cDNA libraries generated paired-end reads, and were prepared using TruSeq stranded mRNA library kit (Illumina, California), and the sequencing was done by Macrogen Inc, (Seoul, Korea), using NovaSeq system (Illumina).
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