RNA-seq?of?V.?vulnificus?MO6-24/O?:?WT_1
Total RNA was isolated using an miRNeasy Mini kit (Qiagen) according to the manufacturers instructions. mRNA was selectively enriched by depleting ribosomal RNA using a Ribo-Zero rRNA Removal kit (Epicentre, Madison, WI). A cDNA library was constructed from the enriched mRNA using the TruSeq Stranded mRNA Sample Preparation kit (Illumina, San Diego, CA) according to the manufacturer's instructions.
WT_1