GSM5290430: RNA_dmso_rep3; Mus musculus; RNA-Seq
For bulk RNA sequencing of etoposide-treated primary neurons, cultures were collected in Trizol LS (cat no. 10296028, Thermo Fisher Scientific, Waltham MA). mRNA was purified and extracted using Direct-zol RNA MicroPrep kit according to manufacturer's instructions. RNA was submitted to the MIT BioMicro Center for library preparation and sequencing. Libraries were prepared using NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (cat no. E7770, New England Biolabs, Ipswich MA). Single-end sequencing was performed using the Illumina NextSeq500 platform according to standard operating procedures.