RNA-Seq of shoot apical meristem of EB3
The quantity and purity of equally mixed RNA were analyzed using Bioanalyzer 2100 and RNA 6000 Nano LabChio Kit (Agilent, CA, USA). Poly (A) mRNA was isolated by poly-T oligoattached magnetic beads (Invitrogen). Following fragmentation, the cleaved RNA fragments were reverse-transcribed into a cDNA library following treatment with the mRNASeqample Preparation Kit (Illumina, San Diego, USA). After assessing the library quality, we performed PE300 sequencing on an Illumina Hiseq 4000 at the LC Sceiences (Hangzhou, China) following the vendors recommended protocol.