GSM5851321: 70925-14; Zea mays; RNA-Seq
Total RNA of DAP 24, DAP 35 and mature kernels were extracted using BAIXU Maize Kernel Total RNA Extraction Kit (Beijing, China). To eliminate any residual genomic DNA, total RNA was treated with RNase-free DNase I (New England Biolabs) and the concentration of RNA were determined using Nanodrop-2000. Total RNA was used to synthesize ﬁrst-strand complementary DNA (cDNA) using the RevertAid First Strand cDNA Synthesis kit (Fermentas). RNA-Seq library preparation and sequencing were carried out by BEIJING BAIXU BIOTECH CO., LTD, Beijing, China. All libraries were sequenced using the Sanger method by Illumina 1.9 Platform. The adapters, low-quality reads, and the reads containing poly-N were removed to obtain clean reads. Then the clean-reads were mapped to Zea mays B73 genome by using Hisat software (v2.1.0) with setting non-strand-specific and other default parameters. Afterwards, the clean reads were assembled using Sam tools software, Cufflink software (v2.2.1) and Cuffcompare software (v2.2.1).