GSM5412788: PCR_NGS-Liver-No_treat-2; Mus musculus; OTHER
Total RNA was extracted using the AllPrep DNA/RNA Mini Kit with on-column DNA digestion using the RNase-Free DNase Set. Oligo d(T)23 VN was used to reverse-transcribe mRNA into cDNA using the High-Capacity cDNA Reverse Transcription kit. KAPA HiFi HotStart ReadyMix was used to amplify a portion of the Idua cDNA spanning the nonsense mutation (W401X in mouse and W402X in human). Amplicons were purified with DNA Clean & Concentrator Kit and subjected to next-generation sequencing by Massachusetts General Hospital CCIB DNA Core under standard conditions. amplicon NGS