16S metagenome from Hermetia illucens
Genomic DNA extraction was done using the Isolate II DNA extraction kit (Bioline, London, UK) as per the manufacturers protocol. The purity and concentration of the resultant DNA was determined using a Nanodrop 2000/2000c spectrophotometer (Thermo Fischer Scientific, Wilmington, USA). Library preparation was performed using the Ligation Sequencing Kit (SQK-LSK108) and Native Barcoding Kit (EXP-NBD103) for genomic DNA, according to the standard 1D Native barcoding protocol provided by the manufacturer (Oxford Nanopore Technologies, Oxford, UK). The constructed library was loaded into the Flow Cell R9.4 (FLO-MIN106) of a MinION device (Oxford Nanopore Technologies, Oxford, UK), which was run with the SQK-LSK108_plus_Basecaller script of the MinKNOW1.7.14 software, for 4 h.