GSM5257990: OPCs-DMSO-rep2; Rattus norvegicus; RNA-Seq
Total RNA was extracted from OPCs using TRIzolTM Reagent (#15596026, InvitrogenTM). Then mRNA was purified form total RNA using poly-T oligo-attached magnetic beads. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illlumina® (#E7770, NEB) and index codes were added to attribute sequences to each sample. The index-coded samples clustering was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumia) according to the manufacturer's instructios.