GSM5839161: splenic_treg_pool; Mus musculus; RNA-Seq
Mechanically dissociated spleen depleted of CD8, B220, and red blood cells via negative magnetic enrichment was sorted via flow cytometry of a BD FACSAria II to isolate the input cell population. Cells were sorted into 1xPBS + 50% FBS prior to hashtag staining then resuspension in 1xPBS + 5% FBS @ 3000 cells/uL for single cell library preparation. The 10x Genomics Chromium Next GEM Single Cell 5' Kit (v1) was used to generate library