GSM5289652: 3F_apob_severe_rep4; Schmidtea mediterranea; RNA-Seq
Total RNA was extracted from 5-6 uninjured planarians per biological replicate using Trizol as described (Forsthoefel et al., eLife, 2020). Total RNA for 4-6 biological replicates per condition was submitted to GENEWIZ (South Plainfield, NJ) for library generation from ~ 500 ng/sample using the NEB NEXT ULTRA Library Prep Kit and RNA sequencing with standard Illumina adapters.