Illumina HiSeq 3000 sequencing
Replicate3: BE(2)C cells treated with Negative Control Mimic (Qiagen YM00479903)
BE(2)C cells were reverse transfected in technical triplicates with 30 nM of Negative Control Mimic (Qiagen YM00479903). Total RNA from transfected cells was extracted using the TRIzol reagent (Invitrogen). RNA was purified and concentrated utilizing RNA Clean & Concentrator-5 kit (Zymo Research)