GSM6046752: s664_L1_fat; Mus musculus; RNA-Seq
Brains (cross-sectional brain region encompassing hippocampal and cortical tissue) and inguinal fat depots were removed, flash frozen in liquid nitrogen, and RNA was harvested using Trizol reagent. RNA isolation using miRNeasy (Qiagen cat. # 217004)  was used with 200 ng of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using TruSeq RNA Library Prep Kit v2 (Illumina)