GSM5413915: WT_1; Geobacter sulfurreducens; RNA-Seq
For each biological sample, total RNA were extracted using Rneasy mini kit (Qiagen), then were examinated with Agilent 2100 Bioanalizer and quantified using NanoDrop 200c (Thermo Scientific). Illumina sequencing was performed at the UUSMD (UNAM, México). After removing residual DNA using DNaseI (Thermo Scientific) and ribosomal RNA with Terminator 5´-Phosphate-dependent exonuclease (Epicentre). The mRNA-enriched was chemical fragmented to 150~200 bp. Based on these cleaved RNA fragments, cDNA was synthesized using a random hexamer primer and reverse transcriptase. After end reparation and ligation of adaptors, the products were amplified by PCR, further purified, and used to create the final cDNA library. Libraries were sequenced on an Illumina Genome Analyzer IIx