GSM5387780: primary mammary organoids - 1 uM CHIR99021 - rep3 ("CHIR_1uM_max"); Mus musculus; RNA-Seq
RNA was extracted using Trizol and RNA was extracted using the Qiagen RNeasy column purification kit. RNA quality was checked with a Bioanalyer (Agilent). PolyA+ stranded RNA library preparation was performed using the Illumina TruSeq stranded RNA prep kit according to the manufacturer's protocol. RNA libraries were prepared for sequencing using standard Illumina protocols. RNA-sequencing was performed on a HiSeq 2500 (Illumina) System using a stranded protocol.