DNA-Seq of Coelomomyces lativittatus CIRM-AVA-1-Meiospore
Infected mosquitos with advanced infections were collected and allowed to lyse.  Samples were incubated at 4C for approximately 2 months, and then sporangia were collected and washed.  After incubation in antibiotic-containing water for 48-72 hours (room temperature), samples of meiospores were collected by centrifugation.  Meiospore samples were cleaned of remaining sporangia by spinning in a table-top mini-fuge for 3 seconds.  Meiospores were then pelleted by centrifugation at 16kxg for 2 minutes. DNA was extracted with Qiagen extraction kit and Qiagen genomic tip20 columns and amplified with the Qiagen repli-G Whole Genome Amplification Kit.  Illumina Libraries were prepared with the NEB DNA Library Prep Kit