GSM4805197: replicate 1 of mdx condition; Mus musculus; RNA-Seq
Eosinophils from infiltrates in mouse quadriceps muscle were isolated by fluorescence-activated cell sorting (FACS); live eosinophils (CD45+CD11c-Gr1-MHCII-SiglecF+) were sorted directly into TRIZOL-LS (Life Technologies, Frederick, MD, USA) at a 3:1 ratio of TRIZOL-LS to sorted cell sample which was then equilibrated with chloroform. Total RNA was purified from the aqueous phase, combined with equal volumes of 70% ethanol, and purified with the RNeasy Cleanup kit (Qiagen, Germantown, MD, USA). RNA libraries were prepared using the NEBnext Ultra Low Input Total RNA Library Prep Kit (New England BioLabs, Beverly, MA, USA) following the manufacturer's instructions.