GSM5851315: OCI-Ly18 - CAR19 AND Venetoclax treatment; Homo sapiens; RNA-Seq
Subcutaneous tumor xenografts of OCI-Ly18 were resected from two mice on day 14 of treatment with either CART19 (Sample 1) or CART19 + Venetoclax (Sample 2). Resected tumors were minced and dissociated into single-cell suspensions using a 0.45 µm filter Libraries for single-cell RNA sequencing were prepared using the Chromium Single Cell 5' Reagent Kit with v1.1 Chemistry (10x Genomics) according to the manufacturer's instructions. After library construction, both libraries were sequenced together on the Illumina NovaSeq 6000. The raw scRNA-seq data were pre-processed using the Cell Ranger software (version 5.0.1) (10x Genomics). Feature-barcode matrices were obtained after aligning reads to the pre-built GRCh38 human reference genome. Filtered gene expression was processed using the Seurat package (version 4.0.1)