GSM5575886: 306XYF; Mus musculus; Bisulfite-Seq
Hippocampi were dissected from XXF, XXM, XYF, and XYM FCG mice (n=3/group), snap frozen in microfuge tubes with liquid nitrogen, and stored at -80ºC prior to DNA isolation. Nucleic acids (DNA/RNA) were isolated from flash-frozen tissues using the All Prep DNA/RNA Mini Kit (Qiagen, Germantown, MD). Whole genome oxidative bisulfite sequencing libraries were prepared according to manufacturers guidelines (Ovation Ultralow Methyl-Seq Library System, Tecan Genomics, Inc., Redwood City, CA). Whole Genome Oxidative Bisulfite Sequencing (WGoxBS-Seq)