GSM6084322: 2-SM-vumc-GFP-dox_S2; Homo sapiens; RNA-Seq
RNA was extracted from cell cultures after 72 hours of treatment with or without 0.5ug/ml Doxycycline. RNA was isolated from 0.5-1.0×106 cells. Total RNA was prepared using RNeasy Mini Kit with DNase I (Qiagen). RNA concentration and quality (A260/A280) was measured using a NanoDrop 1000 Spectrophotometer (Thermo Scientific) as well as TapeStation Analysis (Agilent). RNA libraries were prepared for sequencing using standard Illumina protocols