GSM5838034: NKO mice_snRNA-seq_NKO_male rep1; Mus musculus; RNA-Seq
Lineage tracing experiment: Hypothalamus were dissected from Tbx3-CreER::Ai14 mice (E9 > P12). Then Tbx3-derived cells were isolated by FACS and sequenced by scRNA-seq. Tbx3 knockout experiment: Tuberal and mammillary zones of hypothalamus were dissected from NKO mice and its littermate control. Then tissue were dissociated into single cell for scRNA-seq, or were ground to power to extract the nuclei for snRNA-seq. CUT&Tag experiment: Hypothalamus were dissected from NKO mice and its littermate control and ground to power to extract the nuclei for CUT&Tag. We took advantage of DNBSEQTM technology platforms (BGI Genomics, China) and DNBelab C4 Single-Cell Library Prep Set (MGI, #1000021082) for single-nucleus RNA library preparation. And we followed the manufacturer's recommendations for the 10x Genomics 3' Gene Expression v3 chemistry to prepare single-cell RNA library. For CUT&Tag library, DNA samples were processed with TruePrep DNA Library Prep kit (Vazyme; #TD501-01) according to manufacturer's instructions.