small RNA-seq of  seeds: Juglans regia
10 g of total RNA was ligated with 3' adapter and 5' adapter with the T4 RNA ligase. RNA was reverse-transcribed to synthesize double-stranded cDNA using SuperscriptII reverse transcriptase. DNA fragments were enriched by PCR. Then, products were separated using PAGE gel by fragment size. Single-end sequenced on the HiSeq 2500 platform.