GSM5849744: NT-ICM_RNA_rep1; Mus musculus; RNA-Seq
10 blastomeres were used per reaction, and three replicates were performed for each stage. All isolated blastomeres were washed three times in 0.5% BSA in PBS solution to avoid contamination. RNA-seq libraries were generated using the Covaris DNA shearing protocol for Smart-seq sequence library generation. Briefly, RNAs with a polyadenylated tail were captured, reverse transcribed and pre-amplified. DNA was sheared and purified for library construction. Libraried were generated using the KAPA HyperPrep Kit for the Illumina platform (kk8504), following the manufacturer's instrunctions. Paired-end 150 bp sequencing was performed in NovaSeq (Illumina) platform in Berry Genomics and Novogen.