GSM5155719: WT5 [Bisulfite-Seq]; Arabidopsis thaliana; Bisulfite-Seq
ground tissue was used for DNA extraction, using DNeasy Plant Kit (Qiagen, Germany). DNA was sonicated to 100-300bp fragments and purified with the MiniElute PCR Purification Kit (Qiagen, Germany), End Repair Mix was added, and samples were incubated at 20°C. After DNA purification, a single “A” nucleotide was added to 3' blunt ends, DNA was repurified, and methylated adapters were added on 5' and 3' ends. Fragments of 300-400bp were purified with QIAquick Gel Extraction Kit (Qiagen, Germany), treated with Methylation-Gold Kit (ZYMO), PCR-amplified and purified. 150-bp read, paired-end option, with a minimum depth of 60 million reads per sample, with DNBseqTM (BGI-Tech, China)