GSM5729438: L13a knockout replicate 5 (treated by IFN); Mus musculus; RNA-Seq
RNA was abstracted from macrophage Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer  primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library. RNA-seq: The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on  Illumina platforms, according to effective library concentration and data amount.