Microbiome of short-beaked echidna scats
DNA was extracted from the scats using the Qiagen QIAamp Mini Stool Kit. The 16S rRNA gene was amplified using uniquely barcoded 515F and 806R primer pairs. Samples were pooled to equimolar concentration and cleaned using the Agencourt AMPure XP PCR purification protocol; quantified and quality checked by the LabChip GX Touch nucleic acid analyser. Samples were sequenced on an Illumina Miseq at the Australian Cancer Genomic Facility.