Comparison of DNA purification methods during wine fermentation for high throughput sequencing of fungal communities
PCR were done in technical duplicate, using high fidelity Q5 polymerase, and primers BITS (5 ACCTGCGGARGGATCA 3) and B58S3 (5 GAGATCCRTTGYTRAAAGTT 3), targeting a portion of ITS fungal region. Amplicons were purified, and ligated with Illumina adaptators, prior to MiSeq sequencing using 2x150 cycles.