GSM4984357: GFP-positive_TAAR5-Cre&L10-GFP_H3K9me3_Input; Mus musculus; ChIP-Seq
ULI-ChIP-seq of H3K9me3 was performed as previously described (Brind'Amour, J. et al. An ultra-low-input native ChIP-seq protocol for genome-wide profiling of rare cell populations. Nat Commun 6, 6033, 2015.) except for the library preparation portion. For each reaction, 1,000–10,000 cells and 0.25 ug of antibody (ABclonal, A2360) were used. Libraries were prepared with an NEBNext Ultra kit (NEB) and 12–15 PCR cycles.