Genome re-sequencing of Undaria pinnatifida: gametophytes
Genomic DNA was disintegrated into fragments of ~350 bp using ultrasonic waves. End repair and addition of A to 3 ends were performed and adaptors were ligated. After purification and PCR enrichment, the sequencing library was constructed, and its quality was estimated using an Agilent 2100 bioanalyzer and real-time PCR. The libraries were sequenced on an Illumina NovaSeq 6000 platform.