GSM5257902: Ire1af/f cortex Polysome 1; Mus musculus; RNA-Seq
Ribosome fractionation and polysome purification from E18.5 cortex was performed as published protocol (Kraushar et al., 2021).  RNA was isolated using the Relia-prep RNA mini kit (Promega) Stranded Total RNAseq libraries were prepared using TruSeq with Ribo-Zero Gold rRNA Removal