GSM5289655: 1F_ctrl_rep3; Schmidtea mediterranea; RNA-Seq
Total RNA was extracted from 6-10 uninjured planarians per biological replicate using Trizol as described (Forsthoefel et al., eLife, 2020). mRNA was enriched from 1 µg total RNA/sample using oligo-dT homopolymer beads, and libraries were generated using the Illumina Truseq Stranded mRNA Library Prep Kit according to the manufacturer's protocol. Final libraries were assayed on the Agilent TapeStation for appropriate size and quantity. Libraries were pooled in equimolar amounts as ascertained by fluorometric analysis, then final pools were absolutely quantified using qPCR on a Roche LightCycler 480 with Kapa Biosystems Illumina Library Quantification Reagents.