GSM4868461: 180130-eGFP-D72-lot3 (CEL-seq2); Mus musculus; OTHER
Total RNA was extracted from cells using Sepasol-RNA I Super G (Nacalai Tesque) and reverse-transcribed with PrimeScript II Reverse Transcriptase (Takara) and either oligo-dT primer or random 6-mer according to the manufacturer's protocols. CEL-Seq2 was carried out as described previously (Harada et al. 2017 Nat Commun, Hashimshony et al. 2016 Genome Biol). Sequencing was performed using HiSeq PE Rapid Cluster Kit v2 (Illumina) in an Illumina HiSeq 1500 system with 15 cycles for read 1 and 36 cycles for read 2 in CEL-Seq2.