GSM6008413: control_hPi grafts_pooled_CRISPR; Homo sapiens; OTHER
Quick-DNA™ Midiprep Plus Kit (Zymo Research, D4075) was used to extract genomic DNA (gDNA). gDNA was submitted to GPP for PCR amplification of the integrated construct containing a barcode sequence, and Illumuna sequencing to determine the abundance of each gRNA in each sample. PCR amplification of the integrated construct containing a barcode sequence, and Illumuna sequencing to determine the abundance of each gRNA in each sample.