GSM6307590: MF3-GEX; Homo sapiens; RNA-Seq
Peripheral blood was collected in green tip herapin tubes or via apheresis (ECP). PBMCs were sorted to CD4+CD26- T cells via STEM CELL kits. The scRNA-Seq libraries were prepared following the protocol provided by the 10X genomics. The cDNA and VDJ-enriched libraries were completed in a single bulk reaction using the 10X Genomics Chromium NextGEM Single Cell 5' v 1.1 and V(D)J Reagent Kits.  50,000 or 5,000 sequencing reads per cell for scRNA-seq or VDJ libraries, respectively, were generated on the Illumina NextSeq 500 instrument.