Transcriptome sequencing of sows vaginal epithelium: sample 3 of day 28 of pregnancy
A total amount of 1 g RNA per sample was used as the input material for RNA sample preparations. Subsequently, according to the manufacturers protocols, NEBNext Ultra RNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, MA) was used to prepare sequencing libraries with index labels. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumia, San Diego, CA) following the manufacturers instructions.