MISeqv2 of Rain fungi: GLBRC BCSE
Rain collectors were left in the field for aproximately one week. Rain was brought back to the lab where 350 mL of rain was vacuum filtered through Supor polyethersulfone 0.2 m 47 mm filters (Pall, Port Washington, NY) . We extracted DNA using a PowerWater kit according to manufacturer protocol. We amplified the ITS2 region using the fungal specific primers ITS9 (GAACGCAGCRAAIIGYGA; [41]) and ITS4 (TCCTCCGCTTATTGATATGC [42]).