GSM5505073: P60_Sup_1; Mus musculus; RNA-Seq
Mice (Pomc-CreER; ROSA26eGFP-L10a) were euthanized and decapitated. Brain was removed from the skull and the arcuate nucleus was isolated from a 2-mm thick coronal slices using a brain matrix. Tissues were then homogenized and the subsequent tissue lysate was subjected to immunopurification steps The ribo-depletion kit (RiboGone, Takara, CA) and SMARTer Stranded total RNA sample prep kit were used to remove excess ribosomal RNA and synthesize cDNA library.