10X shotgun sequencing of extracted rhizosphere metagenome
DNA was isolated using a DNeasy PowerSoil Kit using 0.25 g of rhizosphere soil. The samples were rotated gently for 30 min. DNA fractions from four technical replicates were pooled together. Pooled DNA sample was additionally purified using DNeasy PowerClean cleanup kit. From the purified DNA, a read-cloud library was directly prepared on a 10X Genomics Chromium.