GSM5849846: MDA436_sh29_r3; Homo sapiens; RNA-Seq
Total RNA was isolated using Purelink RNA Mini Kit (ThermoFisher). NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (cat# E7760S) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols on Hiseq 4000 platform.