GSM5849885: RNA-seq data for sample tumor T839; Homo sapiens; RNA-Seq
Tumours RNA were extracted using Triple extraction protocol The pool of libraries was quantified using a qPCR method (KAPA library quantification kit, Roche). The sequencing was carried out using paired-end mode (PE100) on a Illumina Novaseq 6000 instrument, using a custom primer (provided into the Nugen kit) to initiate the Read 1 sequencing. The target number of reads was about 50 million paired-reads per sample.