WGS of K. pneumoniae Ecl8 for hybrid assembly
Overnight cultures prepared from a single colony were inoculated in LB, incubated 37C at 180 RPM and used for gDNA extraction using the Wizard Genomic DNA Purification Kit (Promega, UK) following manufacturers instructions. Whole genome sequencing was performed by NovoGene (Cambridge); 150 bp paired-end short-reads were generated on an Illumina Novaseq 6000 instrument (Illumina, Inc., San Diego, CA) and long reads on a PacBio Sequel instrument.