Amplification of rRNA-ITS gene to investigate the environmental fungal diversity in the Mackenzie Bay (Canada)
Fungal DNA was extracted from filters using the ZymoBIOMICS DNA Miniprep Kit. Amplification of the rRNA_ITS2 region was performed using the sequence specific regions (fITS7/ITS4) using a two-step dual-index PCR approach specifically designed for Illumina instruments (Sequences provided are with already trimmed primers).