GSM5025479: WPF_42; Sus scrofa; RNA-Seq
RNA was extracted from all samples using standards protocols. TRIzol reagent (Life Technologies, Grand Island, New York) was used to isolate total RNA and the QIAGEN RNeasy kit (Qiagen, Valencia, California) was use for purification The RNAseq libraries were prepared with Illumina's 'TruSeq Stranded mRNAseq Sample Prep kit' (Illumina Inc, San Diego, CA, USA). Read 1 aligns to the antisense strand and read 2 aligns to the sense strand. The libraries were sequenced on a NovaSeq 6000. Pair-ended reads of length 150bp were sequenced. Fastq files were generated and demultiplexed with with the bcl2fastq v2.17.1.14 Conversion Software (Illumina).