GSM5930292: COX690A23_IL-12_maf fl/fl CD4-Cre_Day 3; Mus musculus; RNA-Seq
RNA was extracted using the QIAShredder and RNeasy Mini Kit, or RNeasy Micro kit, both with on-column DNase digestion, according to the manufacturer's instructions (Qiagen). RNA-seq libraries were made with total RNA equally pooled from the technical triplicate wells within an independent biological experiment, using the Illumina Stranded TruSeq Library preparation kit V2 and unique multiplexing indexes, according to the manufacturer's instructions (Illumina).