GSM5849929: Control [RMM2369]; Mus musculus; RNA-Seq
Trafficked maternal cells and fetal cells were magnetic and flow sorted from the pool of fetal mononuclear cells. Sorted cells were collected in RLT buffer (Rneasy micro kit, Qiagen) and total RNA was isolated with on-column Dnase digestion and extracted carrier-free. The quality of the total RNA was analysed by Agilent2100 Bioanalyzer, before cDNA library preparation. RNA libraries were prepared for sequencing using standard Illumina protocols