RNAseq of axenic coelastrella at 24 hours
The eukaryotic algae were grown under axenic and bacterial-associated conditions.Total RNA was extracted using Trizol reagent (Invitrogen). RNA was cleaned usingthe RNEasy Mini Kit (Qiagen) and analyzed using Tapestation 2200 (Agilent). Paired-end librarieswere prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina.