ECCITE-seq of human: PBMC
For ECCITE-seq ADT HTO RNA and TCR BCR experiments, we used a combination of antibody:oligo conjugates designed for ECCITE-seq, conjugated as described, and commercially available TotalSeq-C reagents. The 10x Immune Profiling v1 protocol was followed according to manufacturer's instructions for cDNA amplification. For the 5P libraries, the samples were pooled in a ratio of 70% RNA, 12% ADT, 8% HTO, 5% of TCR libraries (with equal amounts of a/ß and ?/d libraries), and 5% of BCR libraries. The libraries were sequenced on an Illumina Novaseq S4 flowcell.