NextSeq 500 paired end sequencing; RNA-seq of C. elegans reared on experimental microbiome (EM) and EM cell-free media against OP50-fed controls
RNA-seq of C. elegans reared on experimental microbiome (EM) and EM cell-free media against OP50-fed controls
Whole day 1 adult C. elegans collected from nematode growth medium plates with 1 of 3 bacterial lawn conditions: E. coli OP50 (control), experimental microbiome, or OP50 supplemented with cell-free supernatant from experimental microbiome   RNA extraction with Trizol and BCP  TruSeq mRNA stranded library preparation method using standard TruSeq adapters