RNA-Seq of Metarhizium acridum ARSEF 324 - P0-3 Mycelium
These samples were generated by growing the fungi for four days in 20 ML erlenmeyer flasks with SDY liquid media while being shaken with the shaking-table set to position 100. After four days cultures were filtered through a Whatman filter paper 5-13 m. The fungal material was collected and flash-frozen in liquid nitrogen, before being pulverized in a tissue lyzer and extracted using a QIAGEN PLANT RNEASY kit.