16S screen of AODJ.161.62
Signatures of potentially associating  bacteria were examined via amplicon sequencing of the V3-V4 region of 16S rDNA from the fungal DNA extractions described previously. Nested PCR was performed to enhance the bacterial signal. Primers 27F (Lane, 1991) and 907R (Lane, 1991) were used to amplify the V1-V5 region of 16S rRNA followed by modified versions of primers 341F (Muyzer, et al. 1993) (5-CCTACGGGNGGCWGCAG-3) and 806R (Caporaso, et al. 2011) (5-GGACTACHVGGGTATCTAATCC-3) to further amplify the V3-V4 region prior to sample barcoding. Samples were sequenced on Illumina MiSeq (2 x 300bp paired end reads).