scRNA-Seq of purified blood monocytes from CHD macaques
Freshly thawed PBMC from control (n=3) and CHD (n=3) animals were stained with anti-CD14, HLA-DR antibodies and sorted for live CD14+/HLA-DR+ cells on a BD FACSAria Fusion. Sorted monocytes were pooled and resuspended at a concentration of 1200 cells/ul and loaded into the 10X Chromium gem aiming for an estimated 10,000 cells per sample. cDNA amplification and library preparation (10X v3 chemistry) were performed according to manufacturer protocol and sequenced on a NovaSeq S4 (Illumina) to a depth of >50,000 reads/cell.