GSM4869228: PBS_1; Mus musculus; RNA-Seq
Colon tissues was isolated and flash frozen in liquid nitrogen. Tissues were disrupted with mortar and pestle in liquid nitrogen and 50 mg tissue was lysed in ice-cold RIPA lysis buffer (Thermo). Lysates were further homogenized via centrifugation through a QiaShredder column (Qiagen). RNA from each tissue was isolated using RNAeasy plus mini kit (Qiagen). cDNA libraries were constructed using NEBnext® UltraTM II RNA Library Prep Kit for Illumina®, using 0.2 µg RNA input, and the mRNA enrichment method