Small DNA viruses identified in pooled fecal samples of Tadarida brasiliensis from Arizona, USA
Viral DNA was extracted from pooled fecal samples (n=7) of Tadarida brasiliensis using the High Pure viral nucleic acid kit (Roche Diagnostics, USA) was used to extract viral DNA. The extracted DNA was enriched for circular sequences using rolling circle amplification with TempliPhi 100 kit (GE Healthcare, USA) and this was then used to generate Illumina sequencing libraries using the TruSeq Nano DNA kit.