GSM5838228: SW480 cells at pH 6.6, replicate 1; Homo sapiens; OTHER
Genomic DNA (gDNA) was purified from two infection replicates from each of the three pHe conditions (pHe 7.4, pHe 6.9 and pHe 6.63). PCR of gDNA to attach Illumina sequencing adapters and sample barcodes was performed using the TKO v3 protocol DNA libraries were constructed according to the TKO v3 protocol. Samples were sequenced on a NextSeq 500 using a 75 cycles NextSeq 500/550 High Output Kit v2.5 (Illumina 20024906).