GSM5220195: 604-ASNS48_5Gy_14day; Homo sapiens; RNA-Seq
Cells were harvested and stored at -80C until extraction. Total RNA was isolated using Qiagen RNeasy Plus Mini Kit. Up to 1 µg of total RNA per sample was used for library construction using the Illumina TruSeq Stranded mRNA library preparation kit. Total RNA samples were assessed for concentration using a Qubit fluorometer (ThermoFisher Scientific, Waltham, MA) and for quality using the 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).  Up to one µg of total RNA per sample was used for library construction using the Illumina TruSeq Stranded mRNA library preparation kit (Illumina, San Diego, CA).  Library concentration was measured with a Qubit fluorometer and library size on an Agilent 4200 TapeStation (Agilent Technologies). Libraries were multiplexed and sequenced on a NovaSeq 6000 (Illumina, San Diego, CA) using 75bp single-end sequencing. On average, approximately 30 million reads were generated from each sample.