GSM5257027: H2_S3: epimastigotes rep2, supplemented with haemoglobin; Trypanosoma cruzi; RNA-Seq
RNA samples of epimastigotes incubated with or without heme were extracted from samples by using TRIzol method. Total RNA was measured using Qubit RNA assay kit (Life technologies, USA). The mRNA enrichment was performed in a NEBNext Poly(A) mRNA Magnetic Isolation Module cDNA libraries were synthesized by Illumina TruSeq RNA Sample Preparation Kit.