Piglet ileum 16S rRNA gene amplicons under the effect of L-glutamine and antibiotics postweaning and transport
Ileal digesta was collected from weaned and transport piglets on d13 (when the animals were fed with L-glutamine and antibiotics) and on d33 (period of treatment withdrawal) after transport. DNA was then extracted from the piglet ileal digesta.  The extracted DNA was used to construct the 16S rRNA gene library by amplifying the V4 16S rRNA gene following the protocol from the Schloss lab. The amplified DNA was then normalized using the SequalPrep Normalization Plate (Invitrogen) and pooled to form a single librart. The Ilumina MiSeq (2x250, paired-end) was used to sequence the 16S rRNA gene amplicons.