GSM5155733: mESC vehicle treated control #1; Mus musculus; ATAC-seq
Cells were lysed in cold lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, and 0.1% IGEPAL CA-630), and the nuclei were pelleted and resuspended in transposase buffer. The Tn5 (Illumina FC-121-1030), and the transposition reaction was carried out for 30 min at 37 °C. After purification, the Nextera libraries were amplified for 12 cycles with NEBnext PCR master mix and purified with a Qiagen PCR cleanup kit. PCR with NEB phusion master mix, 12-15 PCR cycles