Trp53-edited Ba/F3 CRISPR-seq
Partial Illumina compatible adapters with unique barcodes are ligated onto each sample during library construction.  Subsequently, a low-cycle PCR amplification step completes the addition of the full-length adaptor sequences.  Libraries are pooled in equimolar concentrations for multiplexed sequencing on the Illumina MiSeq platform with 2x150 run parameters.