GSM5257886: MUS_RNA_FLen_F2R2; Mus musculus; RNA-Seq
Total RNA was isolated using TRIzol reagent according to the manufacturer's protocol. We recycle unmapped reads from standard RNA-seq data, and selected one of unmapped reads as a model. Then, we design specific reverse transcription primers to capture its both ends separately to produce the 5' and 3' end first strand cDNAs. Second strand cDNAs are synthesized and combined to ultrasonic disrupted. The fragments of double strand cDNAs were subjected to construct library follow the instruction of library preparation kit(Vazyme; ND607).