GSM5412799: mRNA_Het_liver_treat_rep3; Mus musculus; RNA-Seq
mRNA from mouse liver samples was extracted and enriched using oligo(dT) beads followed by rRNA removal using the Ribo-Zero kit. Messenger RNAs were fragmented randomly in fragmentation buffer, then the cDNAs were synthesized by random hexamers primer, after which a custom second-strand synthesis buffer (Illumina), dNTPs, RNase H and DNA polymerase I were added to initiate the second-strand synthesis. After a series of terminal repair, 3' adenosine ligation and sequencing adaptor ligation, the double-stranded cDNA library is completed through size selection and PCR enrichment mRNAseq