GSM5289768: Kidney WT - vehicle_rep1; Mus musculus; RNA-Seq
Kidneys were removed, nephron cells were isolated by mechanical force, collagenase incubation, and multiple washes and filtering. Single cells were individually isolated and lysed in cartridges and mRNA molecules captured on BD Rhapsody mRNA capture beads for reverse transcription. cDNA libraries were prepared with BD Rhapsody WTA and Targeted assays, followed by sequencing using standard Illumina protocols.