Illumina HiSeq 2500 sequencing; RNA-seq comparing WT to HDAC3 knock-out clones in naïve mESCs, as well as SL and EpiLC differentiation conditions at two distinct time points (1 and 2 days)
RNA-seq comparing WT to HDAC3 knock-out clones in naïve mESCs, as well as SL and EpiLC differentiation conditions at two distinct time points (1 and 2 days)
total RNA collected Cells grown in 2iLIF on gelatin coated plates transfected with gRNAs AGTTCTGCTCCCGTTACAC and TGAAACCCTGCATATTGGT, mutant clone RNeasy Mini Kit with on-column DNase digest (Qiagen) TruSeq Illumina mRNA-seq (stranded - high input)