RNA-Seq of Pennycress: embryon 16 DAP of Ames 29531 rep2
Plant were grown in a greenhouse with 21- 25C, 16h/8h photoperiod and 300 mol/m2/s light intensity. Pods were collected at 10 and 16 days after pollination and seeds dissected by removing seed coats and endosperm. The embryos RNA was extracted with the CTAB method followed by two precipitation steps with ethanol/ sodium acetate and lithium chloride, respectively. DNA contamination was eliminated by treating the RNA with Qiagen RNase-Free DNase and followed by a concentration step with RNeasy MinElute Cleanup Kit (Qiagen).