GSM5220241: UM2_TCRbulk_betachain; Homo sapiens; OTHER
Reverse transcription of tumor RNA was performed using random hexamers and SuperScript IV according to manufacturer instruction (ThermoFisher). cDNAs were cleaned using Agencourt RNA clean XP kit (Beckman Coulter). Specific Valpha and Vbeta primers slightly modified were used in 2 semi-nested PCR steps followed by a barcoding step. bulk TCR-seq