GSM5413097: 11-G2-LCMV-M-MDSC; Mus musculus; RNA-Seq
Total RNA was extracted from 0.5-1 million macrophages using the Direct-zol RNA Microprep (Zymo Research, Irvine, CA). RNA quantity was determined using the Qubit 2.0 Fluorometer (ThermoFisher Scientific, Waltham, MA) and the quality was validated using the TapeStation RNA ScreenTape (Agilent, Santa Clara, CA). 500 ng of DNAse I treated, total RNA was used to prepare library for Illumina Sequencing using the Quant-Seq 3'mRNA-Seq Library Preparation Kit (Lexogen, Vienna, Austria). Library quantity was determined using qPCR (KAPA Biosystem, Wilmington, MA). Overall library size was determined using the Agilent TapeStation and the DNA High Sensitivity D5000 ScreenTape (Agilent, Santa Clara, CA). Equimolar amounts of each sample library were pooled, denatured and High-Output