GSM5387467: CD8_Tcf1ko_TCF1_r2; Mus musculus; ChIP-Seq
Naive CD8+ T cells from the spleen were sort-purified, crosslinked and immunoprecipitated with anti-Tcf1 antiserum The DNA segments from ChIP were end-repaired and ligated to indexed Illumina adaptors, followed by amplification by PCR with a low number of cells (18-21 cycles). The resulting libraries were seqeunced with Illumina Hiseq-2000 platform.