GSM5504151: NA-PXD-7-6 (bulk RNA-seq); Mus musculus; RNA-Seq
scRNA-seq: HCC tumor resident cells are isolated by a modified two-step collagenase perfusion method. Tumors from three to five mice at the indicated time points were pooled. Viable tdTomato+ and tdTomato- cells were FACS sorted. Single cells were resuspended in PBS with 0.04% BSA, fixed and rehydrated for scRNA-seq.  Bulk RNA-seq: Tissues were snap frozen and RNAiso Plus (TaKaRa) was used to isolate mRNA from frozen tissues. scRNA-seq: Samples were processed using Single Cell 5' Reagent Kits and Single Cell A Chip Kit (Chromium™, 10X Genomics) following the manufacturer's instructions. A total of 5,000 cells of each sample were loaded into each lane of the 10X chip. Libraries were sequenced on a NovaSeq 6000.  Bulk RNA-seq: 1µg total RNA was used for poly-A mRNA library preparation using KAPA mRNA HyperPrep Kit (KR1352-v3.16) following manufacturer's instruction. Libraries were sequenced on a NovaSeq 6000.