GSM4869037: H82_KRAS_Dox_SB-A485-N1; Homo sapiens; ATAC-seq
ATAC-seq was performed using the Omni-ATAC protocol with slight modifications as below. In brief, cells were resuspended in nuclear lysis buffer (10 mM Tris-HCl pH 7.4; 10 mM NaCl; 3 mM MgCl2; 0.1% NP-40 0.1% Tween-20, 0.01% Digitonin) on ice, then spun down in a cold centrifuge at 600 x g for 10 minutes, resuspended in RSB Tween and nuclei were quantified using Trypan Blue (Invitrogen) on a Countess II (Invitrogen). An aliquot of 50,000 nuclei per sample was transferred to a fresh tube, spun down, resuspended in transposition solution and transposed for 30 minutes at room temperature as described previously74. Libraries were prepared using standard Illumina Nextera indices. Library cleanup and dual-sided size selection was performed using SPRIselect beads (Beckman Coulter) with 0.4X and 1.2X ratios. Sequencing was performed on a NextSeq 500 (Illumina) with 150 cycles on a high-output cartridge in paired-end mode at the Center for Genomics and Health Informatics (CHGI) at the Cumming School of Medicine.