GSM6364356: SLAM-seq_mESC_SETD2-dTAG_dTAG-13_24h_bioRep1; Mus musculus; OTHER
Total RNA was extracted using RNeasy Plus Mini Kit (Qiagen) with DTT supplemented to all buffers. Total RNA was spiked in with ERCC RNA Mix 1 and subjected to thiol-linked alkylation by iodoacetamide. RNA was re-purified by ethanol precipitation. Alkylated RNA was used for 3'-end polyA RNA sequencing library preparation with the QuantSeq 3' mRNA-Seq Library Prep FWD Kit for Illumina (Lexogen) according to the manufacturer's protocol.