GSM5412779: NP1400; Homo sapiens; RNA-Seq
Single-cell suspensions were prepared from nasal polyp tissues which were minced into small pieces and placed into a C tube (Miltenyi Biotec) within a solution of RPMI 1640 (Gibco) containing 10% fetal bovine serum (FBS), 0.02 mg/ml DNase I (Millipore Sigma), and 4 mg/ml collagenase type IV (Thermo Fisher Scientific). The mixture was homogenized using the gentleMACS Dissociator (Miltenyi Biotec) and incubated with MACSmix Tube Rotator. The single cell suspension was loaded onto the Chromium Controller (10x Genomics) using the Chromium single cell 3' Reagent Kit v3 (10x Genomics), and scRNA-seq libraries generated. Sequencing was performed on a Illimina HiSeq 4000 with 75bp pair end reads.