GSM4805358: NT-sgRNA_3d_2; Mus musculus; RNA-Seq
Sample collection protocol: Cells were grown in stem cell conditions (0d) and after 3 days of differentiation (3d). Then, cells were washed twice with PBS and RNA was extracted with Trizol. Total RNA was extracted with Trizol followed by DNase treatment using TURBO DNA-free kit (Life Technologies AM1907) RNA-seq libraries were generated from 500 ng using TruSeq Stranded mRNA library prep (Illumina, 20020594). Indexed libraries were pooled and sequenced on an Illumina HiSeq2500 sequencer in 100 bp single-end mode