GSM5839471: H9_hESCs_TFIIIC; Homo sapiens; ChIP-Seq
Preparation of cross-linked chromatin free of RNA, sonication, and immunoprecipitation was performed as previously described (Di Vona et al., 2015). DNA was quantified with Qubit HS kit (Invitrogen). Sequencing libraries were constructed from 1 ng of immunoprecipitated and input DNA using the Ovation Ultralow DR Multiplex System 1-8 and 9-16 kit (NuGen).