GSM6364370: glioblastoma stem cells, G144; Homo sapiens; RNA-Seq
Total RNA from glioma stem cells was extracted using RNeasy Plus Mini Kit (Qiagen) according to the manufacturer's protocol. RNA concentration was determined on a Nanodrop (Thermo Fisher) and RNA quality was confirmed on a Bioanalyzer 2100 RNA 600 Nano Chip (Agilent) to have a RIN value of >9. Libraries for next-generation sequencing were prepared with 500ng total RNA input using Illumina TruSeq RNA Library preparation kit v2 according to manufacturer's protocol