GSM5258165: DNA_H1299_WT_input; Homo sapiens; ChIP-Seq
Total RNAs were extrated using Quick-RNA  Miniprep kit (Zymo Research) and subjected to RNA-seq service provided by Genomic Core of University of North Carolina, USA . Chromatin lysates were prepared by sonication and protein-DNA complexes were isolated with indicated antibodies followed by purification with PCR purification kit (Qiagen). The  isolated genomic DNA were subjected to high throughput sequencing service provided by the genomic core facility of Nation Yang Ming Chiao Tung University, Taiwan. Multiplexing libraries were prepared for sequencing using Illumina TruSeq Rapid SR Cluster Kit according to the standard protocol.