GSM5729703: 9-mo WT-CD11c- rep2; Mus musculus; RNA-Seq
Total RNA was extracted from FACS-sorted cell pellets using RNeasy Plus Universal Mini Kit, according to manufacturer's instructions (QIAGEN). RNA libraries were prepared for sequencing using standard Illumina protocols and CD11c+ and CD11c- microglial samples of 9-mo old WT mice and 5XFAD mice were processed with SMART-Seq v4 Ultra Low Input Kit for Sequencing for full-length cDNA synthesis and amplification