GSM5025689: E13.5 Zfp57-/+ (M-Z+) mRNA-Seq rep3; Mus musculus; RNA-Seq
Live E13.5 embryo were flash frozen on liquid nitrogen, and RNA was harvested using Trizol reagent. Qualified total RNA was further purified by RNeasy micro kit (Cat#74004, QIAGEN, GmBH, Germany) and RNase-Free DNase Set (Cat#79254, QIAGEN, GmBH, Germany). RNA libraries were prepared for sequencing using VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina®(Cat#NR604-02, Vazyme, China)