GSM5454563: RNA-seq of TdTomato+ cells from Cont5 mouse; Mus musculus; RNA-Seq
Endothelial mouse lung cells were isolated as previously described (Fehrenbach et al, Am J Physiol Lung Cell Mol Physiol 2009). Cells were then separated using the BD FACSAria II cell sorter based their TdTomato+ status. RNAs isolation were carried out using the Qiagen miRNeasy Micro Kit. PolyA stranded libraries were prepared by Genewiz using SMARTseq HT Ultra-Low kit (unstranded).