RNA-Seq of multiple myeloma: Z_JL92neg_miRNA
First,the total RNA of samples were separated by 15% PAGE gel (18-30nt),the separated small RNA is centrifuged by ethanol precipitation,and than the enriched small RNA samples were prepared to build the library according to the method and process of Truseq Small RNA Sample Preparation Kit (Illumina, S-200-0048).