GSM5220675: Mettl3_KO_Neurite_Ctrl_DART_rep1; Mus musculus; OTHER
Soma and neurites fractions were respectively isolated from the top and bottom of the microporous membrane using a cell scraper. RNA extraction was performed with the Qiagen RNeasy Mini plus kit. For all samples, 50ng of total RNA was used for library preparation with the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina. For DART-seq samples, 50ng of total RNA was incubated with 250ng of recombinant APOBEC1-YTH protein for 6 h at 37C prior to library preparation.