NextSeq 500 paired end sequencing; Bulk ATAC-seq data of fibroblasts from healthy mouse tissues
Bulk ATAC-seq data of fibroblasts from healthy mouse tissues
Tissues were obtained and minced, aside from the LN, omentum (neither minced) and bone (decapped, marrow removed and crushed). To isolate flank skin, hair was shaved, adipose tissue was removed and tissue was minced. Pdgfra expressing fibroblasts were isolated and frozen. Cell pellets were resuspended in lysis buffer, pelleted, and tagmented using the enzyme and buffer provided in the Nextera Library Prep Kit (Illumina, cat. FC-121-1031). Tagmented DNA was then purified using the MinElute PCR purification kit (Qiagen, cat. 28004), amplified with 10 cycles of PCR, and purified using Agencourt AMPure SPRI beads (Beckman Coulter, cat. A63882). Library preparation and sequencing was performed by ActiveMotif, Inc.