GSM5219663: Col_Rep2; Arabidopsis thaliana; RNA-Seq
Total RNA was extracted from 0.5 cm tips of primary roots of Col and ore15-2 seedlings at 5 DAP (n = 2 seedlings per genotype)(Welgene, Korea). The RNA integrity number (RIN) of each total RNA sample was calculated using the Agilent 2100 BioAnalyzer System, and samples with a RIN 6.7 or higher were selected for RNA-seq. Next, poly(A) mRNA was purified from 1 µg of total RNA of each sample, according to the manufacturer's standard protocols. The purified mRNA was fragmented using the Illumina TruSeq™ Stranded mRNA LT Sample Prep Kit and reverse transcribed using Superscript III reverse transcriptase. then, sequenced on the Illumina HiSeq 2500 platform.