GSM6083547: V1; Solanum lycopersicum; RNA-Seq
The leaves of tomato plants were cut and immediately transferred into the enzyme solutionnby vacuum infiltration for 30 min.  The samples were then protoplasted for 2 hours at 20℃ on an orbital shaker set at 200 g. Cells were then filtered with a 40 µm cell strainer.The WI solution  was added and shaken vigorously to release the protoplasts. The protoplasts were collected by centrifugation at 200 g, and then washed with the WI solution after which the supernatant was removed by centrifugation. Cell activity was detected by trypan blue staining and cell concentration was measured with a hemocytometer. The scRNA-seq libraries were constructed using the Chromium Single Cell 3' Gel Beads-in-emulsion (GEM) Library & Gel Bead Kit v3.