GSM5220645: rCMT3 methylC-seq plants (Line #1; biorep #3); Arabidopsis thaliana; Bisulfite-Seq
Samples 1 to 26 : Genomic DNA was extracted with Quick-DNATM Micro prep Kit (Zymo D3020). MethylC-seq libraries were generated as previously described (Papareddy et al. 2020). Samples 27 to 35: Total RNA was isolated with TRIzol reagent (Thermo Fisher Scientific). Smart-seq2 mRNA libraries were generated from 1 µl of the 7 µl bent cotyledon embryo total RNA as previously described (Picelli 2013; Hoffman 2019).