GSM6008690: Day0, ATDC5, rep1; Mus musculus; ATAC-seq
Unfixed cells at day 0, 3 and 10 of differentiation were scrapped into cold DME/F12 media and collected in 1.5mL centrifuge tubes. Cells were pelleted (300 X g, 4 oC, 5min), the supernatant removed, and snap frozen in liquid nitrogen. Tn5 reactions were performed on 2 independent biological replicates for each time point and sequencing was performed the Princess Margret Genomics Center (Toronto, Canada). Sequencing was performed using the Illumina Novoseq protocol and generated 75bp single end reads.