GSM4805005: F4322-Meth-2; Rattus norvegicus; RNA-Seq
Hearts were either isolated on day 11 (24 hours after the last saline /methamphetamine injection) or isolated 30 days after the last saline / methamphetamine injection to investigate the effects of a 30 day period of abstinence on myocardial gene expression. Total RNA was isolated using Trizol (Thermo Fisher, Waltham, MA) according to the manufacturer's instructions. RNA libraries were prepared from each heart using the Truseq RNA mRNA library prep kit from Illumina (San Diego, CA).  RNA sequencing (2 x 50 paired end reads; 20 million reads / sample) was performed on an Illumina HiSeq1500 sequencer.