GSM5994334: BL_rep1; Borreliella burgdorferi; RNA-Seq
The samples for RNA-extraction were processed with the Direct-zolTM RNA Minprep Plus Kit (Zymo Research) according to the manufacturer's instructions. An on-column DNA digest was performed with the RNase-free DNase set (Qiagen) directly after loading of the samples. The RNA was eluted in RNAse free water and stored in two aliquots at -80°C. The RNA-quantity was measured spectroscopically and the integrity was assessed by agarose-gel electrophoresis. The library was constructed according to the standard RNAseq library procotol at the EMBL Genomics Core Facility (Heidelberg, Germany).