GSM5025433: SHI-C2 (ATAC-seq); Mus musculus; ATAC-seq
HSPCs were lysed in lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2 and 0.1% IGEPAL CA-630) for 10 min and spun at 4 °C for nuclear preparations. The supernatant was discarded. Nuclei were then incubated with Tn5 transposome and tagmentation buffer at 37 °C for 30 min (Vazyme Biotech) The tagmentation products were purified and amplified by polymerase chain reaction (PCR). Amplification was achieved with 10 cycles by using the following conditions: 72 °C for 5 min; 98 °C for 30 s; thermocycling at 98 °C for 10 s, 63 °C for 30 s and 72 °C for 1 min; and 72 °C for 5 min. Libraries were purified with a PCR purification kit (Qiagen) and fragments were enriched with 0.5× and 1.0× VAHTS DNA Clean Beads (Vazyme Biotech) after the amplification.