GSM5929925: Res_LNCaP-42D_ARNTL_siNT_1; Homo sapiens; ChIP-Seq
Chromatin immunoprecipitations were performed as described previously (Stelloo et al., 2018). Samples were crosslinked in 1% formaldehyde (Merck)  for 10 minutes and subsequently quenched with 2.5 M glycine. Samples were lysed as described (Schmidt et al., 2009) and sonicated for at least 10 cycles of 30 sec on, 30 sec off using a Diagenode Bioruptor Pico. For each ChIP, 5 µg of antibody was conjugated with 50 µL magnetic protein A or G beads (10008D or 10009D, Thermo Fisher Scientific). Antibodies used were FOXA1 (ab5089, Abcam) and ARNTL (ab93806, Abcam). Immunoprecipitated DNA was processed for library preparation using a KAPA library preparation kit (KK8234, Roche)