GSM6008840: Lv2, scRNAseq; Loligo vulgaris; RNA-Seq
Single-cell RNAseq libraries were prepared using a Chromium Single Cell 3' Library & Gel Bead Kit v2 or v3 (10X Genomics), according to the manufacturer's protocol (User Guide). Two chips were loaded with the accurate volumes calculated based on the “Cell Suspension Volume Calculator Table”. The initial single-cell suspension being estimated at >600 cells/μl, we targeted to recover a maximum 10000 cells. Once GEMs were obtained, reverse transcription and cDNA amplification steps were performed. scRNA-seq