GSM5504572: BCR of Z3_E21_LN; Mus musculus; RNA-Seq
Blood was collected by cardiac puncture. After thorough perfusion with PBS lumbar lymph nodes, and spinal cord were dissected, and meninges were detached from the spinal cord using fine forceps. Organs were passed through cell strainers. Samples were enriched for CD45+ cells using a MACS (Miltenyi Biotech) with anti‐CD45 mouse MAb‐conjugated microbeads (Miltenyi Biotech, #130-052-301). 10x Genomics Chromium Single Cell Immune Profiling (v1) 10x Genomics single cell sequencing, without feature barcoding