GSM4868495: WT-Day14-CTX-f2 (BRB-seq); Mus musculus; OTHER
Total RNA was extracted from cells using Sepasol-RNA I Super G (Nacalai Tesque) and reverse-transcribed with PrimeScript II Reverse Transcriptase (Takara) and either oligo-dT primer or random 6-mer according to the manufacturer's protocols. BRB-seq was carried out as described previously (Alpern et al. 2019 Genome Biol) except that cDNA was synthesized with RT primers for CEL-Seq2 instead of those described in the original BRB-seq protocol.. Sequencing was performed using HiSeq PE Rapid Cluster Kit v2 (Illumina) in an Illumina HiSeq 1500 system with 15 cycles for read 1 and 45 cycles for read 2 in BRB-seq for mouse samples.