Single cell RNA-seq analysis of skeletal muscle cells from Prrx1-ERT; tdTomato mice
30 days post Tamoxifen injection, G/P muscles from Prrx1-ERT; tdTomato mice  were cleared off fibrotic and fat tissues and teared into small pieces, which were transferred into a 50 ml tube with 10 ml of Ham's F10 Nutrient mix containing collagenase II (0.2% (W/V)), dispase II (2.4 U/ml), and CaCl2 (2.5 mM) and incubated at 37C for 60 min.  The sample was filtered through a 70 m cell strainer  and the remaining cells were washed and pelleted by centrifugation at 500g for 5 min.  Tomato+ cells were sorted using S3e Cell Sorter equipped with a 561 nm wavelength laser.  Single cell libraries was created using the 10X Genomics Chromium Single Cell 3' Library Kit.