Metagenome of Chelymorpha alternans pupae
DNA library was constructed using the NEBNext Ultra II DNA Library Prep Kit for Illumina (Massachusetts, USA) following manufacturer's instructions. Library concentration was measured using Qubit 1x dsDNA HS Assay Kit (Thermo Fischer PN:Q33231) on a Qubit 2.0 fluorometer and size distribution was assessed on an Agilent 2100 Bioanalyzer using the Agilent High Sensitivity DNA kit (California, USA). Sequencing was performed inhouse on a HiSeq 3000 Sequencing System from Illumina, using the paired-end 150 bp technology and at a depth of ~100 million reads.