GSM5930887: D7_Bex_Input; Mus musculus; ChIP-Seq
Isolated primary proximal tubules were crosslinked with 1% formaldehyde for 15 min at room temperature (RT). Crosslinking was stopped by glycine for 5 min at RT. Cells were lysed using cell lysis buffer (140 mM NaCl, 10% glycerol, 0.5% NP-40, 0.25% Triton X-100, 1 mM EDTA, 50 mM Tris-HCl pH 8.0), and then nuclei were isolated using nuclei lysis buffer (10 mM Tris pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 0.2% SDS). Nuclei were sonicated to generate fragments in the range of 200-500 bp, which were incubated with antibody overnight at 4°C. Libraries were prepared according to VAHTS Universal DNA Library Prep Kit for Illumina V3 Kit (Vazyme, # ND607-02).