GSM5851675: batch 2 AMP-1; Mus musculus; RNA-Seq
Total RNA was extracted with the NucleoSpin RNA XS kit (MACHEREY-NAGEL) following manufacture's instruction cDNA was then synthesized and amplified according to the Smart-Seq2 protocol. Purified cDNA was then fragmented using Tn5 followed by library construction using TruePrep® DNA Library Prep Kit V2 for Illumina.