RNA-Seq of Aspergillus niger
Mycelia grown in bioreactors to midexponential phase were used to isolate RNA using TRIzol reagent (Invitrogen) and puried with NucleoSpin RNA Clean-up kit (Macherey-Nagel) with DNase treatment. Quantity and quality of the RNA samples were determined with a NanoDrop 2000 spectrophotometer and by RNA gel electrophoresis, respectively. Messenger RNA was isolated from the total RNA using NEBNext Ultra Directional RNA Library Prep Kit for Illumina, according to the manufacturers protocol. After fragmentation of the messenger RNA, complementary DNA was synthesized using random primers; after a second-strand complementary DNA synthesis reaction, fragments were ligated to the sequencing adapters.