GSM5290694: Repeat-tagged lacZ-α Illumina Sequencing; Escherichia coli; ncRNA-Seq
The treated RNA was afterwards purified by EtOH precipitation including 1 % (v/v) glycogen. cDNA libraries were created with the NEBNext® Multiplex Small RNA Library Pret Set for Illumunia according to the manufacturer's instructions. 100 ng of input RNA was used and amplified cDNA libraries were separated by native PAGE for size selection of 120-250 bp. The selected sizes were extracted from the gel and EtOH precipitated Illumina HiSeq 2500 sequencing were performed at the Max-Planck Genome Centre, Cologne, according to their protocol.