GSM5454404: ChIP-seq HepG2-5-FU IRF3; Homo sapiens; ChIP-Seq
Chromatin immunoprecipitation (ChIP) was performed as previously described (Sahu et al. EMBO J 2011). For analyzing genomic occupancy of TP53, cells were treated with 350 uM 5-fluorouracil (Sigma) 24 h before harvesting the cells. Briefly, fresh formaldehyde-crosslinked chromatin from GP5d cells was used to immunoprecipitate DNA using Dynal-bead coupled antibodies for TFs or histone modifications and for normal rabbit, mouse and goat IgG, followed by standard ChIP-seq library preparation for Illumina sequencing. The libraries were single-read sequenced on HiSeq4000. The immunoprecipiated DNA was end-repaired, dA tailed and adapter ligated followed by PCR enrichment of adapter-ligated ChIP DNA as described in Sahu et al. EMBO J. 2011 Illumina TruSeq library strategy