GSM5220620: Pt72_Tumor_CD8PlusCD28Plus; Homo sapiens; RNA-Seq
immune cells were extracted using standard protocols as detailed in the study methods and according to kit instructions for isolating from patient speciifc samples. Tissue samples were mechanically digested and DNA and RNA was isolated with an all-prep kit (Quiagen) according to protocol. Library was constructed using Clontech SMART-Seq v4 ultra low input RNA kit according to manufactures protocol