GSM6008829: JCP_Patient_50_SLIT_post_VDJ; Homo sapiens; OTHER
Cultured PBMCs were immediately processed in TRIzol reagent (Invitrogen, Carlsbad, CA). For comparison of good responder and poor responders, 25 ng/ml of phorbol-12-myristate-13-acetate and 1 µg/ml of ionomycin (Sigma-Aldrich) were added at the last 4 h of culture because the number of cells available was small. Singel cell RNA-seq, Single cell TCR V(D)J segments (scVDJ-seq) Libraries were prepared using Chromium Single Cell 5' Reagent Kits v1.1 and Single-Cell V(D)J Enrichment Kit for Human T Cells according to manufacturer's protocol (10X Genomics).