GSM5289546: G 0h repeat1; Larimichthys crocea; RNA-Seq
Total RNA was isolated using TRIzol reagent (Invitrogen, USA), according to the manufacturer's instructions. Genomic DNA was removed using DNase (New England Biolabs), and RNA purity was assessed using the NanoDrop 2000 (NanoDrop Technologies, USA). The integrity of each RNA sample was detected by agarose gel electrophoresis and had an A260:A280 ratio and A260:A230 ratio. The RNA samples were used for the subsequent library construction. standard illumina protocols