GSM6046773: WT.C2; Mus musculus; RNA-Seq
Total RNA was isolated for approximately 25 mg of liver tissue using RNeasy Mini kit (Cat# 74106) from Qiagen (Valencia, CA, USA) . RNA was quantified using Qubit RNA BR Assay kit (Cat# Q10211,ThermoScientific, Waltham, MA) in a Qubit3.0 fluorometer. Library preparation was done using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA) as per the manufacturers instructions. The library preparation and sequencing were performed by the Clinical Genomics Core at the Oklahoma Medical Research Foundation