RNA-seq of mus musculus: embryo
cDNA Synthesis: 1)Purification of mRNA (via oligo(dT) beads), 2) Fragmentation of mRNA with divalent cations and heat , 3) 1st strand cDNA synthesis (randomly primed), 4) 2nd strand cDNA synthesis. cDNA library preparation: 1) DNA fragment end repair (blunt ending of DNA fragments), 2) 3' Adenylation of DNA fragments, 3) Sequencing adapter ligation (utilizing T-A pairing of adapter and DNA fragments), 4) Amplification of library via PCR.