GSM5661601: 02-018; Plasmodium falciparum; RNA-Seq
RNA was extracted as described in Ponts N et al 2012, then digested with DNAse I (Qiagen) and purified using RNEasy MinElute Cleanup kit column (Qiagen). Absence of genomic DNA was assessed by the absence of amplification of p90 gene in qPCR from the direct use of the RNA extract, using Syber Green reagent (Life Technology) on Rotorgene 6000. RNA quality was assessed using Nanodrop (2000c) dosage and Agilent RNA 6000 Pico Kit used with 2100TM Bioanalyzeur (Agilent). RNA-seq libraries were performed by TruSeq Stranded mRNA protocol (Illumina®, California, U.S.A.)