GSM5951753: MSC_Pool; Mus musculus; RNA-Seq
Single-cell suspensions were processed using the 10x Genomics Single Cell 3' v3 RNA-seq kit by Ottawa Hospital Research Institute Stem Core Laboratories. Gene expression libraries were prepared according to the manufacturer's protocol. MULTI-seq barcode libraries were retrieved from the samples and libraries were prepared independently. Final libraries were sequenced on the NextSeq500 platform (Illumina) to reach an approximate depth of 20,000-25,000 reads/cell. 3' scRNA-seq