Illumina MiSeq sequencing; qiita_ptid_7631:12716.R4169
This study aimed to explore the associations between food group intake, faecal microbiota profile and body composition during the period of complementary feeding. Diet was assessed using a quantitative food frequency questionnaire, faecal microbiota profile was assessed using 16S rRNA gene sequencing and body composition was assessed using bioelectrical impedance analysis and dual energy X-ray absorptiometry, in a cohort of 50 infants aged 6 to 24 months of age.
DNA extracted using an initial bead beating step followed by extraction using the Maxwell 16 Research Instrument. Preparation of the 16S library was performed using the workflow outlined by Illumina.