GSM4987117: BerneseMountainDog4 - PBMCs H3K4me1; Canis lupus familiaris; ChIP-Seq
PBMCs were isolated with FICOLL-paque plus. SimpleChIP® Plus Enzymatic Chromatin IP kit, digested chromatin was sonicated, and Abcam #ab8895 and #ab8580 antibodies were used. ChIPseq libraries were constructed using Ovation UltralowSystem V2 1-96 with 15 cycles of PCR amplification. Final libraries were twice purified with Ampure XP PCR Purification Beads (Agencourt). Pooled libraries were run with MiSeq Nano kit version 2.