CITE-seq of human: PBMC: Sample P1_123520013
For CITE-seq ADT, HTO RNA , we pooled together 228 TotalSeq-A antibodies from BioLegend. The 10x 3P v3 protocol was followed according to manufacturer's instructions for cDNA amplification.For 3P libraries, the samples were pooled in a ratio of 80% RNA, 12% ADT, and 8% HTO. They were sequenced on an Illumina Novaseq S4 flowcell.