GSM5851962: PP012; Homo sapiens; RNA-Seq
For single-cell RNA-seq, RNA was extracted and poly(A) mRNA was converted into barcoded cDNA using the 10x Genomics Chromium platform. For bulk RNA-seq, RNA was extracted using the Qiagen RNeasy Micro Kit. Single-cell RNA-seq libraries were constructed using the 10x Genomics Single Cell Gene Expression 3' v2 chemistry for donors D363, D370, Adult Donor A, Adult Donor B, HDL049, HDL055, D381 and the 10x Genomics Single Cell Gene Expression 3' v3 chemistry for donors D457, D471, HDL082. For bulk RNA-seq, library preparation was done using the SMART-Seq® ultra-low input RNA kit (Takara bio).