GSM5024994: Sleep control(female7); Homo sapiens; miRNA-Seq
Total RNA was isolated from 1.5 ml of serum, using the Mirvana Paris kit (Ambion), then miRNAs were isolated using a Bioanalyzer (Agilent Technologies). cDNA library based on 10 ng of small RNAs was prepared using the NEBNext® Multiplex Small RNA Library Prep Set.