SLAF-seq based on pooling DNA of Yangzhou male goose with high marketing weight
Twenty individuals from each of the lowest and the highest male goslings groups were used in the SLAF study based on individual estimated breeding values for marketing weight trait by Beijing Biomarker Biotechnology Co., Ltd. Briefly, the genomic DNA of each sample that passed the test was digested separately. The restriction enzymes (RsaI and HaeIII) were used to digest genomic DNA. The fragment sequence length with 314-364 bp was defined as the SLAF tag. The obtained digested fragment (SLAF tag) was subjected to a 3 A-tailing, connected with Dual-index sequencing adapter, followed by PCR amplification, purification, mixing, and gel cutting to select the target fragment. A total of two multiplexed sequencing libraries were constructed. After sequencing, the libraries were qualified with Illumina HiSeq TM2500.