GSM5255566: ChIP-Cpt1bcKO_3; Mus musculus; ChIP-Seq
The ChIP samples were prepared from FACS-sorted adult CM nuclei with truChIP Chromatin Shearing Kit (Covaris); then immunoprecipitation was performed with H3K4me3 antibody (Diagenode). Precipitated DNA samples were purified by MinElute PCR Purification Kit (Qiagen) and quantified by Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific). 0.5-10ng of DNA was used as input for TruSeq ChIP Library Preparation Kit (Illumina) with following modifications. Instead of gel-based size selection before final PCR step, libraries were size selected by SPRI-bead based approach after final PCR with 18 cycles.