GSM4986763: 201_06h; Bos taurus; RNA-Seq
Core muscle tissue samples from the longissimus thoracis et lumborum were collected at 5 different time points [0 h (45 min post-mortem), 6 h, 24 h, 48 h, and 72 h (drip cooler)] post-mortem from the left-side carcasses above the grading site , and immediately frozen in liquid nitrogen, and then stored at -80 °C temperature until RNA extraction. The RNA was extracted using Qiagen RNAeasy mini kit (Qiagen N.V., Valenca, CA, United States) according to the manufacturer's protocol. RNA libraries were prepared for sequencing using Illumina Stranded mRNA Prep protocol.