GSM5155664: R16_t15min_10mM; Bacillus cereus; RNA-Seq
Bacterial pellets were frozen in liquid nitrogen. The pellet introduce in Lysing Matrix B tubes (MP Biomedicals) and high-speed homogenized in FastPrep-24™ 5G Instrument in presence of phenol. Then impurities were extracted from the aqueous phase in three stages: one step with trizol and two with chloroform. The RNAs in the aqueous phase are then precipitated with isopropanol and dissolved in purified water. Transcriptome library preparation and sequencing was performed by I2BC Paris-Saclay platform on the Illumina NextSeq sequencer to generate paired-end 40 bp reads bearing strand specificity