GSM4985045: bulk RNA-seq of SW620 tumors (replicate C_SW620_2); Homo sapiens; RNA-Seq
Total RNA was extracted from the dissected tumors using Trizol reagent (Invitrogen Life Technologies, Carlsbad, CA, USA) and further purified with RNeasy Plus Micro Kit (Qiagen) in accordance with the manufacturer's instructions. mRNA-libraries were prepared using the Smart-seq2 protocol (Illumina, USA). Samples were sequenced by Next-Seq 500 Illumina sequencer and unstranded single-end mRNA-seq libraries of 76bp  were obtained. An average of approximately 38 millions of reads per sample.