HiC of Oncorhynchus gorbuscha: Spawning male liver
The Arima-HiC 2.0 kit (Arima Genomics, San Diego, CA -- User Guide for Animal Tissues Doc A160162 v00) was used to prepare pink salmon liver tissue following the manufacturer's instructions.  Sequencing libraries were prepared using the Swift Biosciences Accel-NGS 2S Plus DNA library Kit instructions (Arima Genomics, San Diego, CA).  DNA shearing was perfomed using the Covaris LE220 Ultrasonicator with the following settings: PIP=50, Duty Factor = 15, CPB = 200.  The library was split into two equal 10 cycle PCR reactions and pooled after amplification.  Sequencing was performed on the Illumina HiSeq X Ten platform (Illumina, San Diego, California) PE150.