GSM5851072: Maternal-Zygotic wnt11b mutant stage 9 rep1; Xenopus laevis; RNA-Seq
Samples were lysed in RNA lysis buffer containing proteinase K, phenol extracted, isopropanol precipitated and DNAse treated. RNA libraries were made using the Illumina Stranded mRNA Prep, Ligation kit (catalog #20040532) and  IDT for Illumina RNA UD Indexes Set A (catalog #20040553). Following KAPA qPCR, samples were run in one lane of a NovaSeq 6000 SP flow cell, 2x100 paried end reads.