RNAseq of Human_Neuron_TLR3
Total RNA was extracted from primary fibroblasts from patients or healthy controls, with the miRNeasy Mini Kit (QIAGEN), and treated with DNAse (Ambion) to remove residual genomic DNA. RNA-Seq libraries were prepared with the Illumina RiboZero TruSeq Stranded Total RNA Library Prep Kit (Illumina) and sequenced on the Illumina NextSeq platform in the 150 nt, paired-end configuration. Each library was sequenced three times.