Metagenome ITS amplicon sequencing of Erodium botrys (Cav.) Bertol. phyllosphere
Amplicons were generated using PCR with an unique 8bp multiplex identifier tag attached to the primer pairs. Resulting products were size selected for amplicons between 300 and 600 bp. Library was constructed and sequenced using an Illumina MiSeq reagent kit v2 with 500 cycles as 2x250bp paired end reads.