GSM4986725: R442Q 2cell rep3; Mus musculus; RNA-Seq
After removing the zona pellucida, zygotes, late 2-cell embryos and blastocysts were lysed in lysis buffer. For RNA-seq, cDNA was generated using the SMART-seq2 protocol. Then cDNA was fragmented by Tn5 enzyme. Fragmented cDNA was end-repaired, ligated with A base. After A base addition, the pre-indexed Illumina adapters were ligated. cDNA with adapters were enriched for another 11 cycles. Finally,  cDNA libraries were sequenced on the HiSeq X Ten platform.