GSM5505965: A673 ETV6 dTAG cells - 24 hour dTAGV-1 treated - replicate 2; Homo sapiens; RNA-Seq
All RNA-seq experiments were performed using total RNA that was extracted with a silica column-based kit (Qiagen, # 74104) and treated with an on-column DNAse digestion per the kit protocol. ERCC RNA spike-ins were added to each sample for normalization, per kit instructions (Thermo Fisher Scientific, # 4456740). RNA-seq library preparation and sequencing were performed by Novogene (Sacramento, CA, https://en.novogene.com) at a depth of roughly twenty million reads per sample. Per Novogene correspondence, the quality control for the RNA samples was performed using Qubit fluorometric quantitation (Thermo Fisher Scientific) and the Bioanalyzer instrument (Agilent). Libraries were then prepared using New England Biolabs NEBNext Ultra II non-directional RNA Library Prep kit. Library quality and concentrations were assessed with Labchip (Perkin Elmer) and qPCR. Libraries were then sequenced in 150-basepair paired-end fashion on the Novaseq6000 instrument (Illumina).