RNA-Seq of Linum usitatissimum: xylem part enriched in secondary cell walls, opposite side, after 24 hours of gravistimulation
Xylem part enriched in secondary cell walls (5 cm long stem portion, at the 3 cm above cotyledon leaves), opposite side, after 24 hours of gravistimulation. The sample were fixed with liquid nitrogen and stored at 80C until RNA extraction. The total RNA from collected sample was extracted using a TRIzol analogue combined with an RNeasy Mini Kit (Qiagen, Germany). The preparation of cDNA library was performed using the TruSeq Sample Prep Kit v3 (Illumina, USA). Raw reads from run were filtered to remove adapter sequences, contamination and low-quality reads.