GSM5387464: Normal_53_9-3-15_P4; Homo sapiens; RNA-Seq
Total RNA was extracted from MSCs using a QIAshredder homogenizer and the RNeasy plus Mini Kit (Qiagen, Valencia, CA, USA) following manufacturer's protocol.  RNA quality was assessed by Nanodrop 1000 (ThermoFisher Scientific). RNA integrity was determined on an Agilent Bioanalyzer (Agilent Technologies. Santa Clara, CA, USA).  Approximately 100 ng total RNA was utilized per sample.  Polyadenylated RNA was enriched, fragmented, and converted to cDNA with the Illumina TruSeqTM sample preparation kit (Illumina, San Diego, CA, USA).  Sequencing of the cDNA (RNA-Seq) was performed to a depth of more than 20 million reads (100-nt reads per sample) with the Illumina HiSeq2500v4.