GSM5505445: aco2i_ctrl_d1_1; Caenorhabditis elegans; RNA-Seq
RNA extraction was performed as previously described with minor modifications (Jeong et al., 2020). Total RNA in the animals was extracted using RNAiso plus (Takara, Seta, Kyoto, Japan) and was subsequently purified by using 75% ethanol. Libraries were constructed with TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, CA, USA) by Macrogen Inc. (Seoul, South Korea) following the manufacturer's instruction.