RNA-seq from a cardiomyocyte cell line from an unaffected family member harvested at day 3.
Peripheral blood monocytes (PBMC) were reprogrammed with OCT4, KLF4, SOX2 and cMYC expressed from a lentivirus vector, then expanded and differentiated to cardiomyocytes using the matrix-sandwich method. Cardiomyocyte RNA was harvested at days 0, 3, 7, and 30. Sequencing libraries were constructed with the Illumina TruSeq Stranded mRNA Library Preparation Kit; polyA RNA was selected with oligo dT magnetic beads, chemically fragmented, reverse transcribed using random primers, and sequenced on an Illumina HiSeq 2500.