GSM6364128: RNA-seq_mESC_NSD1-dTAG_nontreated_bioRep3; Mus musculus; RNA-Seq
Total RNA was extracted using RNeasy Plus Mini Kit (Qiagen) and ERCC RNA Mix 1 was spiked in. Poly(A) RNA was selected using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB). Strand-specific RNA libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) according to the manufacturer's protocol.