GSM6203039: Retic_ac4C_80S; Oryctolagus cuniculus; RNA-Seq
Total RNA was isolated from resuspended ribosome pellets with TRIzol Reagent and subjected to RT-qPCR analysis and deep-sequencing Ribodepleted RNA isolated from purified initiation complexes was also sequenced in a MiSeq instrument in paired-end mode at 75 bp read lengths.