GSM6364061: 357; Apis mellifera; RNA-Seq
RNA was extracted with the RNeasy Mini Kit (Qiagen) and assessed on a Nanodrop, Qubit, and Bioanalyzer The RNAseq libraries were prepared with the Kapa Hyper Stranded mRNAseq Sample Prep kit' (Illumina). Read 1 aligns to the ANTISENSE strand and Read 2 aligns to the SENSE strand. The libraries were pooled; quantitated by qPCR and sequenced on one S4 lane for 151 cycles from both ends of the fragments on a NovaSeq 6000.  Fastq files were generated and demultiplexed with with the bcl2fastq v2.20 Conversion Software (Illumina).