GSM5507800: ODcells_input2  (equal mixture of duplicates of si-IKBZ-treated OD cells); Homo sapiens; ChIP-Seq
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Cells used for ChIP-seq analysis were fixed with formaldehyde and ChIP was performed using the SimpleChIP plus enzymatic chromatin IP kit and magnetic beads (Cell Signaling Technologies) following the manufacturer's protocol. Equal amounts of chromatin (estimated as chromatin obtained from 4 x 106 cells) were used for precipitation of protein-DNA complex with ChIP-validated anti-H3K27ac antibody (ab4729: Abcam). After reverse-crosslinking with proteinase K treatment, chromatin was eluted using ChIP DNA clean and concentrator (Zymo Research). Libraries were sequenced using 150-bp paired-end reads on an NovaSeq instrument to a depth of >10 million mapped reads.