GSM5504672: RNAseq of SJMMAML066084_O1-523_SPL_KD025; Mus musculus; RNA-Seq
RNA was harvested using the Qiagen AllPrep Kit (Qiagen AllPrep Micro Kit for Low input samples) and RNA was quantified using the Quant-iT RiboGreen RNA assay (ThermoFisher) and quality checked by the 2100 Bioanalyzer RNA 6000 Nano assay (Agilent) or 4200 TapeStation High Sensitivity RNA ScreenTape assay (Agilent) prior to library generation. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Library Prep Kit according to the manufacturer's instructions (Illumina, PN 20020599). For the low input samples, cDNA was generated using Tecan/NuGen Ovation RNA-Seq System V2 (catalog 7102) with 1ng RNA, sheared to 300bp using Covaris, and libraries were prepped using the Kapa Hyper kit/protocol (catalog KK8504) and using Illumina True Seq IDT DNA UD Indexes (20020178). Low input library quality was evaluated on a D1000 tape using Agilent's Tape Station 4200.