GSM5851663: Rat1_TKA; Rattus norvegicus; RNA-Seq
Total RNA was extracted using the PureLink RNA Mini Kit (#12183018A, Invitrogen/Thermo Fisher Scientific) following the manufacturer's instructions, and treated on column to degrade contaminating DNA with PureLink DNase (#12185-010, Invitrogen/Thermo Fisher Scientific).  Purified total RNA was eluted from the column with RNase-free water. mRNA-Seq was performed by Novogene (Davis Lab, Sacramento CA), sequencing libraries were generated and subsequently tested for their quality (Library QC) with Qubit, real-time PCR for quantification and BioAnalyzer for size distribution detection.  The Illumina NovaSeq platforms were used for a paired-end 150 bp sequencing strategy to sequence the cDNA libraries.