GSM5387723: KO-S2: STZ-treated KO 2; Mus musculus; RNA-Seq
Tissue acollection was performed from mice sacrificed at ZT9 (ZT – Zeitgeber time units; ZT0 is the time of light on and ZT12 is the time of light off). RNA from whole kidneys was isolated with the standard method of Chomczynski and Sacchi (Anal Biochem, 162: 156-159, 1987). RNA-seq libraries were prepared using 500 ng of total RNA and the Illumina TruSeq Stranded mRNA reagents (Illumina; San Diego, California, USA)