GSM5838005: PDAML2-EOC4-ADT; Homo sapiens; OTHER
BM mononuclear cells (BMMCs) were purified from BM aspirate using density centrifugation, cryopreserved, and stored in liquid nitrogen. CD3+ T cells were purified from cryopreserved BMMCs from AML patients. The 10x Genomics 5' Single Cell Immune profiling platform was used for scRNA-seq of enriched T cells. Reverse transcription of mRNA into barcoded first strand cDNA, as well as construction of gene expression (GEX), VDJ, and antibody-derived tag (ADT) sequencing libraries was performed according to the experimental protocol and reagents provided by 10x Genomics without modifications. The 10x Genomics 3'v3 Single Cell Immune profiling platform was used for scRNA-seq of BMMCs. cDNA amplification was performed according to manufacturer's instructions with the addition of 1 ul 0.2 uM ADT additive primer per sample to amply ADT tags.