Evaluation of partial Nitrification and Anammox processes for the treatment of slaughterhouse byproducts wastewater
The extraction of genomic DNA was carried out according to Griffths (2000). The DNA concentration and purity were evaluated using a Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific, Wilmington, USA), considered indicative of nucleic acid purity (Desjardins and Conklin, 2011). The universal primers 341F (CCTAYGGGRBGCASCAG) and 806R (GGACTACNNGGGTATCTAAT) were used to amplify the 16S DNAr of bacteria. The library was prepared using the NEBNext Ultra DNA Library Pre Kit for Illumina (New England Biolabs, Ipswich, USA) following the manufacturer's recommendations. The library was sequenced on the Illumina HiSeq . The readings were mounted and filtered (QPhred ? 33) to remove sequences containing low quality bases. Chimeras were detected (UCHIME algorithm) and eliminated. After the alignment of the sequences, the UPARSE-OTU algorithm was used to determine operational taxonomic units (OTUs), with 97% similarity