GSM4805185: Twinned siblings from rep3; Xenopus laevis; RNA-Seq
The lysates were subjected to total RNA extraction on NucleoSpin (R) RNA columns (Macherey-Nagel). The obtained RNA samples were tested on integrity on Aligent 4200 TapeStation instrument. For preparation for sequencing, the samples were processed with TruSeq Stranded mRNA Library Prep Kit (Illumina) according to Manufacturer's protocol. The resulted libraries were tested by qPCR and Aligent 4200 TapeStation.