GSM5255573: Ctrl_1_4weeks_after_TAM; Mus musculus; RNA-Seq
RNA was extracted from isolated adult CMs using the Direct-zol Total Kit (Zymo Research) combined with on-column DNase digestion (DNase-Free DNase Set, Qiagen) to avoid contamination by genomic DNA. RNA and library preparation integrity were verified LabChip Gx Touch 24 (Perkin Elmer). 200ng of total RNA was used as input for SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Clontech) following the manufactures instructions.