GSM5506356: miR-125b-Gata1s-FLC+Arid3a-FLAG [ATAC-seq]; Mus musculus; ATAC-seq
We performed ATAC-seq as previously described (Buenrostro et al., Curr Protoc Mol Biol 2015, Buenrostro et al., Nat Methods 2013). 48 hours post-induction, 50K cells were sorted and processed via the Illumina Tagment DNA Enzyme and Buffer Kit (20034197). Illumina libraries were constructed from the transposed DNA as previously described (Buenrostro et al., Curr Protoc Mol Biol 2015, Buenrostro et al., Nat Methods 2013), and sequenced by Novogene Company, Ltd. on an Illumina NovaSeq platform (150 bp paired-end reads).