NextSeq 500 paired end sequencing; RNA-seq of human prostate cancer cell lines grown as orthotopic tumour models of castration resistant prostate cancer
RNA-seq of human prostate cancer cell lines grown as orthotopic tumour models of castration resistant prostate cancer
Cryo ground 1/4 of snap frozen orthograft tumours in liquid nitrogen QIAshredder homogeniser columns (Qiagen, Hilden, Germany), RNA Easy mini kit (Qiagen), on-column DNase digestion (RNase-Free DNase Set, Qiagen), Quality assessed on an Agilent 2200 Tapestation using RNA screentape, Illumina Ribo Gold depletion, Illumina TruSeq Stranded mRNA LT Kit, Qubit quantitation (Thermo)