GSM5839349: HEK293T- Nuclear fraction RNA Rep1; Homo sapiens; RIP-Seq
RNA was isolated using a standard Trizol (Life Technologies) RNA extraction. RNA was DNAse treated before libraries were prepared for sequencing. cDNA libraries from two independent biological replicates were prepared from 400ng RNA using Illumina ribodepleted RNA stranded kit. Libraries were sequenced on the Illumina NovaSeq 6000, paired-end reads (100 nucleotides).