scRNA-seq of homo sapiens: 210825 TCR PBMC
For the 5 gene expression library, the cDNA pool goes through an end repair process, the addition of a single A base, and then ligation of the adapters. The products are then purified and enriched with PCR to create the 5 Gene Expression Library. The purified libraries were quantified using qPCR according to the qPCR Quantification Protocol Guide (KAPA, MA, USA) and qualified using the Agilent Technologies 4200 TapeStation (Agilent Technologies, CA, USA). And then the libraries were sequenced using the HiSeq platform (Illumina, CA, USA).