the whole transcriptomic sequecing of Cynoglossus semilaevis: female muscle
Briefly, mRNAs and ncRNAs were retained by removing rRNAs. The cDNA fragments were reverse transcribed with random primers by DNA polymerase I, purified with QiaQuick PCR extraction kit (Qiagen, Venlo, The Netherlands). After end repaired, poly(A) added, and ligated to Illumina sequencing adapters, cDNAs were sequenced on Illumina HiSeqTM 4000 by Guangzhou Genedenovo Biotechnology Co. Ltd.