Perirenal fat RT-4
Total RNA was purified from perirenal fat with RNAisoPlus reagent (Takara, Tokyo, Japan). RNA integrity was assessed by the Agilent Bioanalyzer 2100 system (Agilent, CA, USA). A total amount of 1 g RNA per sample was used as material for the RNA sample preparations. mRNA was enriched with magnetic beads with oligo (DT), after which the first cDNA strand was synthesized with random hexamers. The purified double-stranded cDNA was ligated for sequencing adaptors and finally sequenced by the novaseq 6000 (Illumina, CA, USA).