RNA-Seq of gallus gallus: embryo male muscle
After rRNA depletion, second-strand cDNA synthesis, RNase H digestion, and adding adaptor in the 3 end, the cDNA library for sequencing was constructed by PCR amplification. Then the cDNA library qualification was detected and sequencing was performed on an Illumina Hiseq 2500 platform and 150 bp paired-end (PE150) reads were generated.