GSM4974364: 9_Brain_smallRNA; Rattus norvegicus; miRNA-Seq
Total RNA and microRNA samples were extracted from tissue or blood using the Lexogen SPLIT Kit ( https://www.lexogen.com/split-rna-extraction/) according to the manufacturers instructions. Briefly, samples undergo a phenol/chlorophorm phase seperation to purify the RNA on a column, followed by size exclusion to seperate any small RNA samples from large RNA. Libraries were prepared according to Lexogen's instructions accompanying the smallRNA & Quantseq library preparation kits. Briefly, RNA was diluted following a Lexogen SPLIT KIT RNA extraction and  samples were loaded with equal concentrations of either total or small RNA. Total RNA libraries were generated according to the manufacturers instructions here: https://www.lexogen.com/wp-content/uploads/2015/11/015UG009V0211_QuantSeq-Illumina.pdf --- small RNA libraries  were prepared following the manufacturers instructions here: https://www.lexogen.com/wp-content/uploads/2020/02/052UG128V0110_Small-RNA-Seq-Library-Preparation-Kit_2020-01-22.pdf. The purified DNA libraries were processed on an Illumina flow cell for cluster generation. Libraries were sequenced on the Illumina HiSeq 3000 following the manufacturer's protocols.