GSM4986874: GC092448: Retina control rep 2; Mus musculus; RNA-Seq
Retinas were isolated, snap-frozen in liquid nitrogen and stored at -80°C until use. The snap-frozen neural retinas were first homogenized in Trizol, with subsequent RNA extraction using the PureLink RNA Mini Kit, including on-column DNase treatment. The sequence libraries were prepared with the QuantSeq 3' mRNA-Seq Library Prep Kit (Lexogen), according to the manufacturer's protocol. Samples were indexed to allow for multiplexing. Library quality and size range were assessed with the DNA 1000 kit (Agilent Technologies), using a Bioanalyzer (Agilent Technologies).