GSM4804467: Replicate 3 siCNOT1 1hr; Homo sapiens; RNA-Seq
Cells were washed with ice-cold PBS and Trizol added directly to the well. The RNA was extracted with Trizol and acid-phenol purification Total RNA was poly(A) selected using the lexogen poly(A) selection kit. 4ng poly(A) selected RNA was then used as input into the lexogen CORALL Total RNA-seq kit and 10 PCR cycles used to produce the libraries.