GSM5158862: si287_2; Homo sapiens; RNA-Seq
1 μg of total RNA was enriched for the Poly-A mRNA and reverse transcribed to double-stranded cDNA. The cDNA was end repaired, adenylated, and ligated with Illumina indexes to construct cDNA library which was paired-end (PE) sequenced using Illumina sequencing platforms based on Next-Generation Sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols.