GSM4986864: 12_neg_ASH_GAGTGG; Gallus gallus; RNA-Seq
Cranial portions of chicken heads were dissected and dissociated using Accumax. For each sample, around 20 heads were pooled for FACS sorting. GFP+ neural crest and GFP- embryonic head cells were captured by directly sorting into RNA Aqueous micro kit. Stranded RNA-Seq was performed with NEBNext® Ultra™ II Directional RNA Library Prep Kit (NEB #E7765) according to manufactuors' instructions.