MedExome sequencing of KRASG12C mutant RW7213 Parental and KRAS G12C inhibitor (AZ'1569) acquired resistant cells
The Roche KAPA HyperPlus kit (Roche, USA) was used for whole genome library preparation, followed by the Roche SeqCap EZ MedExome Probes in conjunction with the HyperCap Target Enrichment Kit to enrich for targets. Targeted DNA sequencing was performed (200ng genomic DNA was used)on the Illumina NovaSeq 6000 applying paired-end sequencing with 2x 100bp reads.