Chicken Cecal Metagenome
The cecal sample was aseptically collected from a randomly chosen bird, transferred to sterile Whirl-Pak plastic bags (Nasco, Fort Atkinson, WI), immediately frozen (-20C), and transported to the laboratory for taxonomy analysis. Metagenomic DNA isolation from the ceca samples was performed using QIAamp DNA Stool Mini Kit (Qiagen, Venlo, Netherlands)) with modification as previously described (Zaheer, Noyes et al. 2018, DOI: 10.1038/s41598-018-24280-8).