GSM5155412: LNCaP_shDouble_R1881_Rep2; Homo sapiens; RNA-Seq
After the above 4 hour treatments, total RNA was collected using the 5Prime Perfect RNA Cell Kit (Fisher Scientific) according to the manufacturer's protocol, including the optional DNAase treatment After quality control, RNA samples were enriched for mRNA using oligo(dT) beads, fragmented for a 250-300 bp target range, then cDNA synthesis using random hexamer primers, second strand synthesis is performed using dNTPs, RNAas H and DNA polymerase I in Illumina synthesis buffer, Illumina sequencing adapters, then the double-stranded cDNA library is size selected and PCR enriched.