GSM5024989: Single celll RNA sequencing data; Mus musculus; RNA-Seq
PPs were incubated for 20 min at 37°C with 10 mM ethylenediaminetetraacetic acid buffer and then digested for 45 min at 37°C with collagenase D/DNase I. After removing dead cells using a dead cell removal kit CD11c+ or CD8+ cells were enriched using anti-mouse CD11c microbeads or a CD8+ T cell isolation kit We utilized the 10X Genomics Chromium Instrument and cDNA synthesis kit (10x Genomics) to generate a barcoded cDNA library for single cell RNA-sequencing from approximately 8,500 cells. cDNA library quality was determined using an Agilent Bioanalyzer. Using this library, we performed one full lane sequence on two paired-end 150bp Flow Cells using on an Illumina Nextseq550.