GSM5257978: PBMC_SA_polyIC_4; Homo sapiens; RNA-Seq
After 24 hours of incubation, cells were harvested into Eppendorf tubes and centrifuged for 10 min at 400g. Cells were washed once with 1 mL PBS with 0.01% BSA (PBS-BSA) to remove remaining FBS, then centrifuged for 8 min at 400g. After discarding the supernatant, cells were then resuspended with 1 mL plain PBS. An aliquot of cells was removed for cell counting and viability recording with a hemocytometer using the trypan blue exclusion method to select samples with cell viability greater than 90%. Cells were adjusted to 140-160 live cells/µL using PBS-BSA as Drop-Seq input. The Drop-Seq experiment was carried out with the version 3.1 protocol from the McCarroll Lab