RNAseq of Solanum lycopersicum: wt root in 6 days P starvation and 1 day GR24 treatment under P starvation condition
Total RNA from root samples was extracted using the RNA sample preparation kit RNeasy Mini Kit (QIAGEN) combined with TRizol reagent (Invitrogen), and genomic DNA was digested using RNase-Free DNase (Qiagen, USA). RNA integrity was evaluated by 1.0 agarose gel electrophoresis. Total RNA was quantified with a Nanodrop 2000 (Thermo scientific, Wilmington, USA) and samples were used for RNAseq library construction only when the OD 260/280 was higher than 1.8 and the OD 260/230 higher than 2.32.