MISeqv2 of Leaf fungal endophyte: Switchgrass Seedling
Leaves from seedlings were harvested.plant samples were surface sterilized by placing material (either seed or leaf) in a tissue cassette and submerging the sample in a series of 1min in nanopure water, 1 minute in 70% ethanol, 5 min in 10% bleach, and 1 min in 90% ethanol [40]. Plant material was extracted using Plant DNeasy kit with standard manufacture protocol and an extended lyses step. We amplified the ITS2 region using the fungal specific primers ITS9 (GAACGCAGCRAAIIGYGA; [41]) and ITS4 (TCCTCCGCTTATTGATATGC [42]).