GSM5151400: Lyn KO / Irf5 Het rep4; Mus musculus; RNA-Seq
Total RNA from peripheral blood stabilized in RNAprotect Animal Blood Tubes (Qiagen) was purified using the RNeasy Protect Animal Blood Kit (Qiagen). Libraries were prepared from 500 ng of total RNA using SureSelect Strand-Specific RNA Library Prep for Illumina Multiplexed Sequencing (Agilent Technologies). Pooled libraries were subjected to the sequencing using the Illumina NextSeq 500/550 High Output Kit v2 (75 cycles) and NextSeq 500 System.