Blue-fronted amazon female muscle Hi-C
The chromatin was cross-linked and digested using the restriction enzyme MobI, and then blunt-end-repaired, and tagged with biotin. The DNA was religated with the T4 DNA ligation enzyme. After ligation, formaldehyde crosslinks were reversed and the DNA purified from protein. biotin-containing DNA fragments were captured and used for the construction of the Hi-C library. Finally, 350-bp paired-end libraries constructed from DNA were sequenced on the Illumina HiSeq X Ten platform