GSM5257744: ATAC-seq, cell line MUN14, replicate 3; Homo sapiens; ATAC-seq
DNA was purified using a MinElute PCR purification kit (Qiagen) and eluted in 10 l Elution Buffer for library amplification.  PCR amplification of tagmented DNA was done using the NEBNext HiFi PCR mastermix (New England Bioloabs) with a universal forward and sample-specific reverse oligo for sample barcoding using the following PCR conditions: initial incubations of 72C for 5 min and 98C for 30 s, followed by 5 cycles of 98C for 10 s, 63C for 30 s, and 72C for 1 min. Additional number of cycles was determined for each sample through a “side” qPCR reaction using an aliquot of the PCR as template to determine the number of cycles needed to reach 1/3 of the max fluorescence. Total cycle numbers ranged from 8 to 9 cycles. PCR products were run on a 1% agarose gel, regions from ~50 bp to ~1 kb were excised, and DNA was extracted using a gel extraction kit (Qiagen). PCR amplification of tagmented DNA was done using the NEBNext HiFi PCR mastermix (New England Bioloabs) with a universal forward and sample-specific reverse oligo for sample barcoding