RNA-Seq of Ulva prolifera: adult thalli
The mRNA with ploy(A) structure was enriched with oligo magnetic adsorption. The enriched mRNAs were fragmented, then reversed into a double-strand cDNA (dscDNA) with an N6 random primer. Sequencing adaptors were linked to the end of the purified dscDNA, and the ligation product was amplified by PCR with specific primers. The PCR product was thermally denatured into single-stranded, and then the single-stranded DNA was circularized with a bridge primer to obtain single-stranded circular DNA library.