GSM5413939: HFSCs-control4; Mus musculus; RNA-Seq
Clipped mouse dorsal skin was harvested and fat tissue was removed by gentle scalpel scrapping. Skin samples were then floated on 0.25% trypsin solution for 2 hours at 37℃ or overnight at 4℃. Then epidermis was detached from the dermis by gentle scalpel scraping and minced to prepare primary keratinocytes single-cell suspension, which was then filtered through a 70-µm cell strainer. Hair follicle stem cells were FACS-sorted based on expression of CD34 and CD49f. Libraries were generated using the Nextera XT DNA Library Prep kit (Illumina).