GSM5851382: K27-ccc1∆-1; Cryptococcus neoformans var. grubii; ChIP-Seq
ChIP DNA was extracted by bead beater with 0.5mm zirconia beads.  ChIP DNA was isolated by an antibody against H3K27me3 (described in PubMed ID 25533783). DNA was then isolated by incubation with SDS/proteinase K followed by column purification (Macherey-Nagel Nucleospin Gel Cleanup Columns). NEBNext® Ultra™ II DNA Library Prep with Sample Purification Beads (E7103, NEB) was used according to the product manual.