GSM4984616: M1_R1; Homo sapiens; RNA-Seq
RNA was purified from primary human macrophages after the above timepoints, using Trizol and isopropanol precipitation For ChrRNA-Seq, libraries were generated using NEBNext Ultra II Directional Prep Kit for Illumina (New England Biolabs), according to the NEB's protocol. For 3'RNA-Seq, libraries were generated using QuantSeq REV kit (Lexogen), according to Lexogen's instructions.