GSM6084378: Cut&Run on Ctnnb1 in Trim71 wild-type mESCs IgG; Mus musculus; OTHER
Total RNA were isolated from mESCs via Trizol reagent The Trim71(R783H) CLIP-seq was performed using the previously established HITS-CLIP protocol (Moore et al., 2014). The cut&run was performed using the CUT&RUN Assay Kit (Cell Signaling Technology, Cat#86652) according to its instruction manual. CLIP-seq, RNA-seq, and Cut&Run