Whole genome sequencing of Sparidentex hasta
One microgram genomic DNA was randomly fragmented by Covaris. The fragmented genomic DNA were selected by Agencourt AMPure XP-Medium kit to an average size of 200 - 400 bp. Fragments were end repaired and then 3 adenylated. Adaptors were ligated to the ends of these 3 adenylated fragments and the fragments were amplified using PCR. The PCR products were purified by the Agencourt AMPure XP-Medium kit. The double stranded PCR products were heat denatured and circularized by the splint oligo sequence. The single strand circle DNA (ssCir DNA) were considered as the final library. Library was validated on the Agilent Technologies 2100 bioanalyzer. The qualified libraries were sequenced by BGISEQ-500: ssCir DNA molecule formed a DNA nanoball (DNB) containing more than 300 copies through rolling-cycle replication. The DNBs were loaded into the patterned nanoarray by using high density DNA nanochip technology. Finally, pair-end 150 bp reads were obtained by combinatorial Probe-Anchor Synthesis (cPAS).