GSM6360721: rSPL10 (r10)-Pto biological replicate 2; Arabidopsis thaliana; RNA-Seq
Total RNAs were extracted using E.Z.N.A. Total RNA kit (Omega BIO-TEK). RNA quality was assessed with a 2100 Bioanalyzer instrument (Agilent, RIN score ≥ 7, 28S/18S ≥ 1). RNA concentration was measured using a Nanodrop spectrophotometer (Thermo Scientific, RNA concentration ≥ 50 ng/μL, 260/280 ~2.0). RNA samples were sequenced at BGI, San Jose lab. Oligo dT based mRNA enrichment was followed by random N6 primer based reverse transcription. Synthesized cDNAs were then tailed with an Adenine each and then ligated with a bubble adapter. The DNA products were further amplified through PCR, separated with heat and cyclized using splint oligo. The synthesized DNA nanoballs were then sent for non-stranded mRNA sequencing (PE100) on a DNA Nanoball Sequencing (DNBseq) platform.