GSM4869080: OI-5; Homo sapiens; RNA-Seq
Total RNA extraction was performed with a commercial Tempus Spin RNA Isolation Kit (Ambion, Life Technologies Corp., Carlsbad, CA, USA) following manufacturer's protocol. A GLOBINclear™ Kit (Ambion, Life Technologies Corp., Carlsbad, CA, USA) was used to purify the samples of globin mRNA. The quality of total RNA was assesed using a capillary electrophoresis approach Agilent 2100 Bioanalyzer and RNA 6000 Nano kit RNA-seq library construction and RNA sequencing analysis were performed with a SOLiD 5500W protocols SOLiD 5500 Wildfire (W) System chemistry (Life Technologies Corp., Carlsbad, CA, USA) was applied to prepare the resulting cDNA for the DNA fragment library. Next, the 12 libraries were pooled together in equal amounts to construct two different library pools. The pooled libraries were converted to SOLiD 5500W libraries.