GSM6046889: Zebrafish heart , 7dpa, DN, H3K4me3; Danio rerio; ChIP-Seq
About 25 zebrafish hearts were pooled for each ChIP experiment. The hearts were dissected from adult zebrafish, and the outflow tract and atrium were removed. Chromatin isolation and ChIP assays were performed using a Pierce Magnetic ChIP Kit (Pierce). Anti-H3K4me3 (Abcam) antibodies were used for the ChIP assays. The DNA bound by ChIP was used for ChIP-seq library construction. ChIP-seq libraries were prepared using the VAHTS Universal Pro DNA Library Prep Kit (Vazyme) for Illumina. 5 nanogram of DNA was used as starting material for input and IP samples. Libraries were amplified using 13 cycles on the thermocycler. Post amplification libraries were size selected at 250-450bp in length using Agencourt AMPure XP beads from Beckman Coulter. Libraries were validated using the Agilent High Sensitivity DNA Kit (Agilent).