RNA-Seq of Callogorgia delta preserved in situ, biological replicate 1
Total RNA was isolated using a Qiagen RNeasy Kit or a modified Trizol/Qiagen RNeasy protocol. Library preparations were performed by the sequencing facilities on high quality (RIN values > 7) RNA samples (Illumina Truseq RNA library kit). Sample preserved in situ on the seafloor were further multiplexed and sequenced across Illumina HiSeq2000 flow cells at the University of Wisconsin Biotechnology Center (UWBC, Madison, WI) sequencing facility to acquire 150 bp paired-end reads at approximately 30 million reads per sample.