GSM6008412: sBWH7 [re-used]; Homo sapiens; RNA-Seq
For co-cultured experiment, cells were collected from 96 wells after 48hrs of co-cultured and SC-islets dissociated using TrypLE Express at 37 °C, to form single cells and quenched with S3 media. Harvested cells from both sources were centrifuged, resuspended in 0.04% BSA in PBS, counted (LUNA-FX7 Automated Cell Counter), adjusted to 1000 cells/μl and sent to the Harvard University Bauer Sequencing Core for 10X Chromium Single Cell 3' Library preparation and sequencing. 10X Chromium Single Cell 3' Library preparation and sequencing