GSM5220541: RS411 nascent RNA-seq control rep3; Homo sapiens; RNA-Seq
108 4sU-treated cells were lysed in 10ml Trizol. RNA was extracted using chloroform and precipitated with EtOH. 4sU RNA was biotinylated with 1mg/ml Biotin-HPDP and nascent RNA was enriched for using streptavidin-coated beads. DNA libraries were prepared using the NEB Next Ultra II Directional RNA library preparation kit for Illumina (E7765).