GSM5414127: hALEC_d0_2; Homo sapiens; RNA-Seq
RNeasy extraction kit (Qiagen). For bulk RNAseq, total RNA was purified using RNeasy kit (Qiagen) followed by treatment with Turbo DNase (Thermo Fisher Scientific). 500ng-1mg RNA was reverse transcribed with SuperScript II system and oligo-dT primer (Thermo Fisher Scientific), followed by 8 cycles of amplification using KAPA HiFi HotStart ReadyMix PCR Kit (KAPA Biosystems, Roche). 100-200pg of DNA were used for tagmentation performed with Nextera XT kit (Illumina), followed by 10 cycles amplification whereby iNext sequencing adaptors were added. The libraries were purified using AMPure XP magnetic beads (Beckman Coulter). The quality of the libraries was tested using Agilent Bioanalyzer system.