GSM5661565: Grass puffer brain; Takifugu alboplumbeus; OTHER
Staining, permeabilization, reverse transcription, cDNA amplification, and library construction were conducted following Visium user guide (Rev. C) with Visium spatial gene expression reagent kits (10X Genomics). Staining, permeabilization, reverse transcription, cDNA amplification, and library construction were conducted following Visium user guide (Rev. C) with Visium spatial gene expression reagent kits (10X Genomics). After HE staining, HE image was immediately captured (BZ-X800, Keyence). Time for permeabilization was 12 min with thermal cycler (T100, Bio Rad). The number of cycles for cDNA amplification was 14 with real-time qPCR system (Quant Studio 3, Applied Biosystems).