GSM5930041: 1299_Input; Homo sapiens; ChIP-Seq
Lysates were clarified from sonicated nuclei and nuclear protein/DNA complex was isolated with antibody. The ChIP procedure was performed as illustrated in the ChIP kit (#9002S, CST, Danvers, MA). Briefly, the tested cell lines were fixed with formaldehyde, and cross-linking was performed by adding glycine. The samples were placed on ice and sonicated to separate the DNA into 100 to 1000-bp fragments. Then, they were incubated with antibodies at a dilution of 1:50 overnight, followed by incubation with protein G agarose beads at 4 °C overnight. Next, the bound DNA-protein mixtures were eluted, and cross-linking was reversed after several washes. The precipitated chromatin DNA was then collected and analyzed by ChIP-seq aligned to human genome (Seqhealth Co., Wuhan, China).