GSM5289680: CO; Homo sapiens; RNA-Seq
Organoids were dissociated and subset of cell suspension was stained with 0.4% Trypan Blue (Gibco) and assessed for viability and concentration using Countess II Automated Cell Counter (Invitrogen).  Single cell suspensions were passed through 40µm cell strainer (Corning) and concentration was adjusted to 1000 cells/µL. Suspension were loaded in single-cell-B Chip (10X Genomics) for target output of 10,000 cells per sample. Single-cell droplet capture was performed on the Chromium Controller (10X Genomics). Library preparation was performed in accordance with the Single-Cell 3' v3 protocol.