GSM5258132: SUDHL1_RG2_H3K27ac_ChIPseq; Homo sapiens; ChIP-Seq
The crosslinking was reversed overnight at 65 °C. Input and ChIPed DNA was cleaned up and submitted to Novogene (Sacramento, CA) for libraries construction and sequencing. Immunoprecipitated DNA sample quality was assessed by DNA Tapestation (Agilent Technologies Inc., California, USA) and quantified by Qubit 2.0 DNA HS assay (ThermoFisher, Massachusetts, USA). The KAPA HyperPrep kit (Roche, Indianapolis, USA) was used for library preparation and indexing was performed using Illumina 8-nt dual-indices. Final libraries were quantified by Qubit 2.0 (ThermoFisher, Massachusetts, USA) and run on TapeStation D1000 ScreenTape (Agilent Technologies Inc., California, USA) to evaluate the quality of the purified libraries. Final library size was approximately 300 bp. Equimolar pooling of libraries was performed based on QC values and sequenced on an Illumina NovaSeq S4 (Illumina, California, USA) with a read length configuration of 150 PE for 80M PE reads per sample (40M in each direction).