GSM6046865: Cell_DMSO2; Mus musculus; RNA-Seq
Three hundred thousand PMN-MDSCs from each condition were lysed in 5 mL 10 ´ lysis buffer. SMARTer cDNA synthesis starts with picogram amounts of total RNA or single cell/several cells. Amplify sscDNA by LD PCR and get enough dscDNA for library construction. cDNA was fragmented by dsDNA Fragmentase (NEB, M0348S) by incubate at 37°C for 30min. Library construction begins with fragmented cDNA. Paired-end sequencing on an Illumina Novaseq™ 6000.