Illumina HiSeq 2000 paired end sequencing; ATAC-seq Analysis of Chromatin Accessibility in p53 Deficient Spermatogonial Stem Cells for High Frequency Transformation into Pluripotent State
ATAC-seq Analysis of Chromatin Accessibility in p53 Deficient Spermatogonial Stem Cells for High Frequency Transformation into Pluripotent State
SSCs were isolated from 5-day old p53+/+ or p53-/- mice testes using two-step enzymatic digestion and purified by FACS.The THY1+;c-kit- fraction was collected for centrifugation and collection. ATAC-seq library processing was performed according to the Omni-ATAC protocol.