GSM5387390: EZH2 KO DAY12 LGLUT biological replicate 2, technical replicate 3; Homo sapiens; RNA-Seq
Total cell RNA was prepared from the cells using RNAeasy reagents (Qiagen). 3' mRNA-Seq libraries were prepared with the Lexogen QuantSeq 3' mRNA-Seq (FWD) kit.  Total RNA was quantified using via the RNA QuantiFluor RNA system E3310 on the GloMax (Promega) Multi Detection system following the maufacturer's guidelines.  RNA quality was checked via the High Sensitivity RNA ScreenTape using the TapeStation 4200 (Agilent).  RNA was normalised to 30 ng and used for cDNA synthesis and library preparation using the Lexogen QuantSeq 3' mRNA-Seq (FWD) kit according to the manufacturer's instructions.  Libraries were sequenced on the Illumina HiSeq 4000 with single ended reads