GSM5929863: CD11c+ CD11b+ DCs, 8 Gy radiotherapy, B2; Mus musculus; RNA-Seq
Leukocytes in the irradiated tumors were harvested and dispersed into single-cell suspensions on day 17. CD11b-CD11c+, CD11b+CD11c+, and CD11b+CD11c- myeloid populations and CD8 T cells were isolated from the irradiated tumors using cell sorters, and RNA was extracted immediately after cell sorting. To increase the RNA yields and sample representativeness, we pooled the tumor-infiltrating leukocytes from 10 tumors (1 tumor per mouse) for each group. RNA libraries were prepared for sequencing using standard Illumina protocols