GSM5257833: ALS_WholeTissue_SOD_Input_rep4; Mus musculus; RNA-Seq
Motor cortex (or whole cortex for samples 15-21) was dissected and homogenized and tagged polysomes were immunopurified with monoclonal anti-EGFP antibodies bound to biotinylated-Protein L (Thermo Fisher) coated streptavidin-conjugated magnetic beads (Life Technologies). Total RNA from IPs was purified with the Agilent Nanoprep kit following manufacturer's protocol. Total RNA from "input" samples was purified from the pre-IP tissue homogentates using the Agilent Nanoprep kit. 15 ng total RNA from TRAP IP or input was amplified using the NuGEN Ovation RNA-Seq System V2 and libraries were prepared from 10 ug amplified cDNA using the Illumina TruSeq RNA Sample Preparation Kit v2 following manufacturer's protocols.