RNA-seq of filtered marine shellfish intake water
Filters were used for RNA extraction by MoBio Power Water RNA isolation kit following manufacturer's instructions, including the alternate lysis step. Output total RNA was depleted for rRNA and prepped for RNAseq using the ScriptSeq Complete Gold (Epidemiology) kit (illumina). 50 ng of ribosomal depleted RNA was used as an input to transcriptome libraries. Individual libraries were randomly pooled into groups of four samples using equimolar quantities and sequenced on a MiSeq v3 600 reagent kit to generate paired-end 250 bp reads.