Linked-read metagenomic sequencing of human HCT patient stool sample
DNA was extracted from stool samples using a mechanical bead beating approach and the QIAamp Fast DNA Stool Mini Kit. DNA fragments less than approximately 2kb were eliminated with a SPRI bead purification with a custom buffer. Sequencing libraries were prepared on the 10X Genomics Chromium platform and sequenced on an Illumina HiSeq 4000.