Nanopore sequencing of Nephila pilipes: Adult female cephalothorax
Following the amplification process, PySp1 DNA sequence length was verified. Target sequences were gel extracted following the standardized protocol of the Agarose gel extraction kit (TIANGEN, DP209-02). DNA was then applied directly in Oxford Nanopore 1D Genomic DNA based on the Adaptor Ligation protocol (SQK-LSK109). Fast5 files were bascalled using Oxford Nanopore program Guppy (v3.6.1+249406c).