C3|starved
RNA was extracted from the muscle by using TriZol (Invitrogen, Carslsbad, CA, USA), followed by quantity and quality assessments. Total RNA was treated with DNase I to degrade DNA contamination, if any. Then mRNA was enriched using the oligo (dT) magnetic beads. The mRNA was fragmented and then random hexamer-primer was used to synthesize the first strand of cDNA. The second strand was synthesized followed by end repair, 3-end single nucleotide A (adenine) addition, and adapter ligation. The fragments were enriched by PCR amplification. Finally, single strand separation, cyclization, and DNA nanoball synthesis were performed. The library products were sequenced on a DNBseq platform. For microRNA sequencing, 24 libraries were prepared following the manufacturers instructions, and then sequenced on a DNBseq platform.