GSM5387189: Cnp_ko_5_bulkRNA; Mus musculus; RNA-Seq
Mice brain hemispheres (without cerebellum and olfactory bulb) were enzymatically and mechanically dissociated using Miltenyi adult brain dissociation kit under 37 °C for 30 min. Dissociated tissue suspensions were purified with Miltenyi RBC lysis and debris removal reagents. The acquired single-cell suspensions were stained by CD11b beads and passed through the Miltenyi magnetic field for microglia cell enrichment. Further, total RNA was extracted from sorted microglia cells using the RNeasy Micro kit (Qiagen). mRNA libraries were prepared using illumina TruSeq protocol and sequenced by illumina HiSeq 4000