DNA-Seq of Coelomomyces lativittatus CIRM-AVA-1-Amber
Infected copepods of the amber mating type were separated from the orange mating type prior to lysis.  Liquid was removed and then copepods were washed with sterile water to remove any contaminants.  After the gametes were released from the copepods, the copepod carcasses were removed by allowing them to settle in the microfuge tube.  Samples were spun for 3 minutes at 6000xg, supernatant removed. DNA was extracted with Qiagen extraction kit and Qiagen genomic tip20 columns and amplified with the Qiagen repli-G Whole Genome Amplification Kit.  Illumina Libraries were prepared with the NEB DNA Library Prep Kit