Illumina NovaSeq 6000 paired end sequencing; RNA-Seq of zebrafish whole-eye comparing wild-type and mab21l1 c.107delA p.(Lys36Argfs*7) embryos at 5 days post fertilization
RNA-Seq of zebrafish whole-eye comparing wild-type and mab21l1 c.107delA p.(Lys36Argfs*7) embryos at 5 days post fertilization
Whole eyes were dissected from 5 day post fertilization wild-type or homozygous mab21l1-c.107delA embryos into 1X Ringer's solution. Three biological samples were collected per genotype, and each sample contained 50 eyes. To isolate RNA, whole-eye samples were  lysed and homogenized in TRI Reagent. Then, the Direct-zol RNA MiniPrep Kit from ZymoResearch was followed to extract and purify RNA.  RNA Samples were submitted to Macrogen for RNA-Seq performance. Macrogen conducted library prep using a TruSeq Stranded mRNA LT Sample Prep Kit following the TruSeq Stranded mRNA Sample Preparation Guide, Part # 15031047 Rev. E.