GSM6008913: M27; human lung metagenome; OTHER
The bacterial DNA was extracted from human airway samples with a QIAamp Fast DNA stool Mini Kit   (Qiagen, Cat# 51604) and PCR amplification was conducted with barcoded specific bacterial   primers targeting the variable region 3–4 (V3–V4) of the 16S rRNA gene: forward primer 338F:   5'- ACTCCTACGGGAGGCAGCA-3' and reverse primer 806R: 5'-  GGACTACHVGGGTWTCTAAT-3'. Construction of sequencing libraries and paired-end sequencing was performed on an Illumina NovaSeq6000 platform at Biomarker   Technologies Co, Ltd. (Beijing, China) according to standard protocols.