GSM5454643: FO.het.4wk.1; Mus musculus; RNA-Seq
Spleens were harvested at time of saccrifice and lypmhocytes were stained and FACS sorted directly into TRIZOL reagent as follows:  follicular B cell: live, singlet, CD19+, CD93-, CD23+, CD21/35int.  marginal zone B cell: live, singlet, CD19+, CD93-, CD23-, CD21/35high. RNA was extracted by TRIZOL protocol and quantified by Qubit.  cDNA was prepared using Takara Clonetech SMART-Seq HT kit by protocol with Illumina Nextera XT reagents for library construction and quantified and equal molar pooled by tape station and qubit analysis.