GSM5729418: RNA-4sU_KELLY_DMSO_rep2; Homo sapiens; RNA-Seq
RNA was extracted from cells at ~50% confluency using TRIzol reagent. RNA from 4sU-treated cells was thiol-modified by exposure to iodoacetate, or left untreated. Following this step, mRNA was enriched by poly(dT) capture. RNA was fragmented, converted to DNA, then size-selected for 200-400 bp DNA fragments before PCR amplification. All libraries were barcoded and prepared for sequencing using standard Illumina protocols.