RNA-Seq of Ae. aegypti Gut 6h post blood meal and injected with htEcoli
The digestive tracts of 30 adults were collected. Total RNA was extracted using the RNeasy Mini kit (Qiagen) using the manufactures protocol, followed by quantification and quality checking using Agilent 2100 Bioanalyzer. TruSeq mRNA (Illumina) library construction and 75bp paired-end sequencing over three NextSeq High Output flow cells were performed by University of Georgia Genomics and Bioinformatics Core. cDNA for each sample was indexed with a unique adaptor. Each library was normalized by equimolar multiplexing. Pre-processing of raw reads included seqClean adaptor, uniVec database filtering, and poly-A tail trimming, and quality trimming using FASTX-Toolkit and quality was assessed using FastQC.