GSM5155204: 2-265; Mus musculus; RNA-Seq
Tumors were removed, flash frozen on dry ice, and total RNA was harvested from tumor using the RNeasy Plus Universal Mini Kit (QIAGEN). RNA-Seq libraries were constructed after oligo dT-based mRNA enrichment using a NEBNext mRNA Magnetic Isolation Module (New England Biolabs). The RNA-Seq Library Prep Kit (Illumina) was then used according to the manufacturer's instructions.