RNA-Seq of Linum usitatissimum: phloem fibers depositing tertiary cell wall , pulling side, after 24 hours of gravistimulation
Phloem fibers at the later stage of tertiary cell wall deposition (5 cm long stem portion, at the 3 cm above cotyledon leaves), pulling side, after 24 hours of gravistimulation. The sample was fixed with liquid nitrogen and stored at 80C until RNA extraction. The total RNA from collected sample was extracted using a TRIzol analogue combined with an RNeasy Mini Kit (Qiagen, Germany). The preparation of cDNA library was performed using the TruSeq Sample Prep Kit v3 (Illumina, USA). Raw reads from run were filtered to remove adapter sequences, contamination and low-quality reads.