GSM5413961: RAZZ_163; Homo sapiens; RNA-Seq
Whole blood was collected and stored at -80C. Following RNA extraction (Zymo Pathogen Magbead Kit) and DNase treatment, human cytosolic and mitochondrial ribosomal RNA was depleted using FastSelect (Qiagen). RNA was then fragmented and underwent library preparation using the NEBNext Ultra II RNAseq Kit (New England Biolabs). Libraries underwent 146 nucleotide paired-end Illumina sequencing on an Illumina Novaseq 6000 instrument.