GSM5506351: FLAG_CUT&RUNmiR-125b-Gata1s-FLC+Arid3a-FLAG_CUT&RUN; Mus musculus; OTHER
We performed CUT&RUN as previously described (Meers et al., eLife 2019; Skene et al., Nat Protoc 2018). On day 2 post-induction, 0.2 million cells were sorted and incubated  with the following antibodies: rabbit anti-DRIL-1 (abcam), rabbit anti-GATA1 (Abcam), rabbit anti-SMAD2 and anti-SMAD3 (Cell Signalling) and rabbit anti-IgG (Diagenode).  pAG/MNase nuclease (Addgene 123461) was produced and purified as previously described, after removal of the HA tag. Illumina libraries were constructed from the cleaved DNA as previously described (Meers et al., eLife 2019; Skene et al., Nat Protoc 2018), and sequenced by Novogene Company, Ltd. on an Illumina NovaSeq platform (150 bp paired-end reads).