Whole genome sequencing of PantoeaR6
100ng of genomic DNA was fragmented using the Covaris ultrasonicator, selecting for approximately 400 bp fragments. The DNA was end-repaired using the Ion Xpress plus fragment library kit (Thermo Fisher Scientific) and the 2% Agarose E-gel was used to size select for fragment greater than 300 bp. The gDNA size was confirmed using the High-sensitivity D1000 DNA ScreenTape kit on the Agilent TapeStation 4200 (Agilent technologies). Libraries were constructed using the Ion Xpress Kit (Thermo Fisher Scientific) and quantified on the QuantiStudio 12K using the Ion Library TaqMan Quantitation Kit (Thermo Fisher Scientific). The gDNA libraries were diluted to 100 pm and subjected to emulsion PCR using the Ion 520 & Ion 530 Kit-Chef (Thermo Fisher Scientific). The final enriched libraries were loaded on the Ion 530 chip kit and subjected to sequencing on the Ion Torrent 5S GeneStudio.