GSM4805158: N2 rep1 IP; Mus musculus; RNA-Seq
RIP was performed using Magna RIP Kit (Millipore) following the manufacturer's instructions and a Ythdf3 antibody targeting mouse endogenous Ythdf3 protein. The input and immunoprecipitated RNAs were recovered using RNAiso plus and extracted according to the manufacturer's protocol. Libraries were constructed using the NEBNext® Ultra II Directional RNA Library Prep Kit (NEB).