GSM5454448: Endothelial cells, GF1; Mus musculus; RNA-Seq
Colon endothelial cells were positive isolated using MACS-Beads (CD45-CD31+) and sorted for ICAM-1+ CD146+. Total RNA from 0,2x10^6 cells was extracted. Quantity of total RNA was assessed with the Qubit 2.0 and quality was checked using a RNA 6000 Pico chip on Agilent's bioanalyzer. Barcoded mRNA seq libraries were prepared from 20 ng total RNA using a NEBNext® Poly(A) mRNA Magnetic Isolation Module and NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® according to the manual. All quality controls were done using Invitrogen's Qubit HS assay and fragment size was determined on Agilent's 2100 Bioanalyzer HS DNA chip.