GSM5506201: RNA-Seq wild-type DD36 rep2; Neurospora crassa; RNA-Seq
Tissue was harvested in the dark, pressed in between paper towels to remove excess media, and immediately frozen in liquid N2. polyA+ RNA was isolated from 100 μg of total RNA with NEB Oligo d(T)25 Magnetic Beads. Purified mRNA (8 ng) was used as the input to generate RNA-seq libraries using the Lexogen SENSE Total RNA-seq Library Preparation Kit (Cat. #009).