GSM5387174: melphalan_NG25_rep1; Homo sapiens; RNA-Seq
Total RNA was then extracted using RNeasy Mini kits including DNAse digestion with Qiacube (QIAGEN, Hilden, Germany, 74104). RNA quantity, quality/integrity and purity were evaluated using Qubit (Thermo Fisher Scientific, MA, USA), Bioanalyzer (Agilent, CA, USA) and NanoDrop (Thermo Fisher Scientific, MA, USA). RNA sequencing libraries were generated for 12 RNA samples, using SENSE mRNA-Seq library prep kit V2, according to manufacturer's instructions (Lexogen GmbH, Vienna, Austria). Libraries were quantitated by qPCR using KAPA Library Quantification Kit (Kapa Biosystems, Inc., MA, USA) and validated using Agilent High Sensitivity DNA Kit on a Bioanalyzer (Agilent Technologies, CA, USA). The size range of the DNA fragments were measured to be in the range of 200-450 bp with average library size 240 bp.