GSM5174208: NK cell - Lung - Treated #2; Mus musculus; RNA-Seq
NK cells were flow cytometrically sorted in RLT buffer (qiagen) +1% b-mercapto-ethanol for the subsequent extraction of total RNA using RNAeasy mini kit (Qiagen). Total RNA was extracted using according to the manufacturer's protocol. Library preparation was performed as previously described (Picelli, S., Faridani, O., Björklund, Å. et al. Full-length RNA-seq from single cells using Smart-seq2. Nat Protoc 9, 171–181 (2014). https://doi.org/10.1038/nprot.2014.006), using 2100 Bioanalyzer System for library quality control.