GSM5507757: Sl1; Botrytis cinerea; ncRNA-Seq
RNAs were extracted with the TRIzol reagent (InvitroGen) and submitted to a DNAse treatment (DNA-free kit, Ambion). Libraries were performed from at least 1µg of extracted total RNA with a RIN greater than 7. Before starting, total RNAs were purified with miRNeasy kit (Qiagen) which allows the selection of the small RNA fraction less than 100 bases. From these samples enriched in small RNAs, libraries were performed according to the protocol published by Vigneault et al. (2012) with adjustments to improve the ligation.