GSM5850520: T54-Treg; Homo sapiens; RNA-Seq
Total RNA was extracted from cell pellet using the Maxwell RSC RNA FFPE kit (Promega) according to the manufacturer's protocol without the step at 80°C. 500 ng of total RNA were ribodepleted and used for library construction. Libraries were prepared thanks to NEBNext Ultra II Directional RNA Prep kit for Illumina according to manufacturer's instructions.