GSM5504622: MD2; Valsa mali; OTHER
Total RNAs were extracted with Trizol TM Reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer's instructions. RNA purity, concentration, and integrity were checked. RNA of three biological replicates of MVm and MD2 were mixed, respectively. And three micrograms RNA of each sample was used to construct sRNA library construction and high-throughput sequencing in LC-BIO (Hangzhou, China). The detailed protocol of miRNA-seq was provided by the genome sequencing company (LC-BIO Hangzhou, China) and the protocol of degradome sequnce was followed with  the published parallel analysis of RNA Ends (PARE) protocol (German et al., 2009)..