GSM6363883: Escort Cells scRNA-seq; Drosophila melanogaster; RNA-Seq
GFP positive cells from C587-GAL4 / yw; UAS-CD8-RFP/+ ovaries were sorted via Fluorescence-activated cell sorting (FACS). The sorted cells were loaded on a Chromium Single cell Controller (10 X Genomics) using the Chromium Single-cell 3' Library & Gel Bead kit v3 according to manufacturer's directions. The raw sequencing data was processed with alignment, barcode assignment and UMI counting by Cell Ranger (version 6.1.1) using the previously published pipeline (61). The reference index for Cell Ranger was built using the Drosophila melanogaster genome (version BDGP6.32) available on the Ensembl genome database.