Swab severe COVID-19 : Cov_2_006
Oral swab sticks were washed with PBS buffer and the DNA of captured bacteria isolated with the Quick-DNA Fecal/Soil Microbe Miniprep Kit. 5 L of the obtained DNA were subjected to the Illumina Nextera XT workflow using the primers 5-TCgTCggCAgCgTCAgATgTgTATAAgAgACAgCCTACgggNggCWgCAg-3 and 5-gTCTCgTgggCTCggAgATgTgTATAAgAgACAggACTACHVgggTATCTAATCC-3 for the first amplicon PCR with 45 PCR cycles. After Nextera v2 index PCR and quality control by capillary gel electrophoresis samples were pooled and sequenced on MiSeq device (Illumina).