GSM5729453: 8505c cells_10mM-DEL-22379-treated_Rep2; Homo sapiens; RNA-Seq
RNA was extracted using the PureLink™ RNA Mini Kit (Thermo Fisher Scientific) Total RNA was assayed for quantity and quality using the Qubit RNA BR Assay Kit on the Qubit 2.0 Fluorometer (Life Technologies), and the RNA 6000 Nano Assay on a Bioanalyzer 2100 (Agilent). The RNASeq libraries were prepared using TruSeq Stranded mRNA LT Sample Prep Kit protocol (Illumina). The libraries were sequenced on NovaSeq 6000 (Illumina) with a read length of 2x51bp following the manufacturer's protocol for dual indexing.