Illumina HiSeq 4000 sequencing; DNA replication profiling by BrdU Immunoprecipitation of S.cerevisiae wild-type versus H3K37R mutant under over-expression of MCM activators.
DNA replication profiling by BrdU Immunoprecipitation of S.cerevisiae wild-type versus H3K37R mutant under over-expression of MCM activators.
standard centrifugation YPA-Raffinose, YPA-Glucose and YPA-Galactose medium at 30C G1 arrest (2h alfa factor at 30C)->YPAGLU/ YPAGAL + 0.2M HU+0.5mg/ml BrdU (1h 15 minutes at 30C)-> 0.1% NaN3 (10 minutes in ice) Genomic Qiagen prep NEXTFLEX® ChIP-Seq Library Prep Kit for Illumina®Sequencing