C. subhashii SUB3 mate-pair sequencing
DNA was fragmented to sizes between 1 and 20 kb using a transposase that binds biotinylated adapters at the breaking point. Strand displacement was performed to repair the nicks left by the transposase. Fragment sizes of 3 to 6 kb were then selected on a 0.8% agarose gel and were then circularized. Non-circularized DNA was removed by digestion. The circular DNA was then mechanically sheared to fragments of 100 bp to 1 kb approx. and the fragments containing the biotinylated ends were pulled down using magnetic streptavidin beads and submitted to a standard library preparation. A final size selection on 2% agarose gel was done and fragments of 400 to 700 bp were selected for the final library.