GSM4987047: LLC_TCR_profiling_KO_pd1_ctla4_treatment_rep2; Mus musculus; RNA-Seq
RNA was  isolated using an RNeasy Micro kit (Qiagen). TCR profiling from RNA was performed using the SMARTer Mouse TCR a/b Profiling Kit (Takara) llumina sequencing primers were added in a semi-nested approach during TCR amplification; libraries were purified, pooled and then sequenced on an illumina Miseq platform