16s rRNA of B. brizantha cv. Marandu (rizosphere): Local L1, Replicate R1
DNA was purified and subjected to next- generation sequencing (NGS) with Illumina MiSeq library preparation by Macrogen (Korea, Seoul). The V3V4 region of the 16S rDNA gene was amplified using 341F (CCTACGGGNGGCWGCAG) and 805R (GACTACHVGGTATCTAATCC) (Herlemann et al., 2011) primers with amplification of approximately 440 bp.