RNA-Seq of homo sapiens: glioblastoma
RNA libraries were generated, sequenced, and processed as previously described (Suntsova et al (2019)). RNA extraction was performed using RecoverAll Total Nucleic Acid Isolation Kit (Invitrogen), and RNA Integrity Number (RIN) was measured with Agilent 2100 bioanalyzer. RNA concentrations were measured using Qubit RNA Assay Kit, and ribosomal RNA was depleted using RNA Hyper Kit with RiboErase (KAPA Biosystem, Roche). Processed library concentrations and length distributions were measured using Qubit ds DNA HS Assay kit (Life Technologies) and Agilent Tapestation (Agilent), respectively. The samples were sequenced using Illumina HiSeq 3000 engine for single end sequencing, 50bp read length, for approximately 30 million raw reads per sample. Data quality check was conducted using Illumina SAV. De-multiplexing was performed using Illumina Bcl2fastq2 v 2.17 software according to (Suntsova et al (2019)).