Illumina RNAseq of Hydractinia symbiolongicarpus: gastrozooids and gonozooids
The RNA-Seq library was constructed from 1 microgram RNA using the Illumina TruSeq Stranded mRNA kit. The resulting cDNA was fragmented using a Covaris E210 focused ultrasonicator. Library amplification was performed using 10 cycles to minimize the risk of over-amplification. Unique barcode adapters were applied to each library.