GSM4971373: AL58 - Hmr_Flag_FL_induced; Drosophila melanogaster; ChIP-Seq
Chromatin immunoprecipitation was essentially performed as in Gerland et al., PlosOne 2017. For each ChIP reaction, chromatin isolated from 1–2 x 10^6 cells was incubated either with 5 ug of mouse anti-FLAG (F1804, SIGMA-ALDRICH - RRID: AB262044) pre-coupled directly to Protein A/G Sepharose or with rat anti-HMR 2C10 (﻿RRID: AB2569849) antibody pre-coupled to Protein A/G Sepharose through a rabbit IgG anti-rat. Libraries for AL02-78 were prepared with Diagenode Microplex Library Prep Kit v2 (starting with 1ng of DNA), AL79-80 with "NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (starting with 2 ng of DNA)