GSM5413117: Lept-1_rep2 [G2021_22_11]; Homo sapiens; RNA-Seq
RNAs from various cells were purified by the Direct-zol RNA kit (Zymo). RNA-seq libraries were prepared using NEBNext Ultra II RNA-Seq Kit (NEB) as per the manufacturer's instruction with duel indexing. Quality of libraries was assessed in Tapestation (Agilent technologies) using D1000 Tape. Libraries were quantified using a well plate reader (Bio Tek) and pooled at a concentration of 12nM. Pooled libraries were quantified using the Droplet PCR system (Bio-Rad), and pooled libraries were sequenced in one lane Nova-Seq S4 as 150 bp Paired-end reads.