Cas12m + pCRISPR NT from TXTL
Quality and integrity of total RNA was controlled on 5200 Fragment Analyzer System (Agilent Technologies). The RNA sequencing library was generated from 100 ng total RNA using NEBNext Multiplex Small RNA Library Prep Set for Illumina to manufactures protocols. For size selection in the range of 167-198 bp was used the Pippin Prep instrument. The libraries were sequenced on Illumina NovaSeq 6000 using NovaSeq 6000 S1 Reagent Kit (100 cycles, paired end run 2x 50 bp) with an average of 5 x106 reads per RNA sample.