GSM5929641: WT4; Mus musculus; RNA-Seq
female PRKO mice at 21-27 days old were hormonally stimulated with 5IU PMSG (44hrs) followed by 5IU hCG and ovaries collected at 8hrs post-hCG. Ovaries were repeatedly and thoroughly punctured to remove GCs and the residual ovary was snap frozen in liquid nitrogen. A total of 4 biological replicates per genotype were obtained, each consisting of tissue pooled from 3 mice. RNA was extracted from the unprocessed residual ovarian tissue using the RNeasy Mini Kit (Qiagen), including DNase treatment, as per manufacturer's instructions mRNA libraries were prepared using the Illumina Truseq Stranded mRNA kit. Paired end, 100bp sequencing was performed on NovaSeq 600 SP, 200 cycle (Illumina) sequencing system with a depth of 54-72M reads per sample