GSM5454509: hematopoietic stem cells from G5/G6 mice treated with the oligonucleotide A151; Mus musculus; RNA-Seq
BM samples were first processed and enriched as described above, and cells were sorted by FACS. Sample preparation and sequencing were performed at MD Anderson's Advanced Technology Genomics Core. Sample concentration and cell suspension viability were evaluated using a Countess II FL Automated Cell Counter (Thermo Fisher Scientific). Samples were normalized for input onto the Chromium Single Cell A Chip Kit (10x Genomics), in which single cells were lysed and barcoded for reverse-transcription. Equal amounts of each uniquely indexed sample library were pooled together. scRNA-seq