GSM4804956: WT primary myoblasts-ATAC rep2; Mus musculus; ATAC-seq
Myoblasts were washed with PBS for one time and digested with typsine. ATAC-seq library was constructed from 5000 cells using TruePre DNA Library Pre Kit V2 for Illumina (Vazyme, TD501-01) and big peaks were filtered out using VAHTS DNA Clean Beads (Vazyme, N411-02) acording to the manufacturer's instructions. These libraries were sequenced on Illumina HiSeq X Ten instrument with 150-bp reads and paired-end parameter by WuXi NextCODE company.