GSM6008446: WT_5H_R2; Saccharomyces cerevisiae; RNA-Seq
Total RNAs were extracted on 2.0 - 3.0 OD of 2 OD/ml yeast cells using a standard hot acid phenol protocol. mRNA-seq libraries were generated from 500 ng of total RNA using TruSeq® Stranded mRNA Library Prep kit and TruSeq® RNA Single Indexes kits A and B (Illumina, Cat#20020595) according to manufacturer's instructions. Quantity and quality of the started material and final librairies were checked using a Qubit™ fluorometer (Invitrogen™) and Agilent Bioanalyzer. Resulting libraries were sequenced in 50-length Single-Read on a HiSeq 4000 machine (Illumina).