GSM6008643: SR2, biological replicate 2; Homo sapiens; RNA-Seq
Total RNA extraction was carried out using a mirVana miRNA isolation kit (Ambion, #AM1560) according to manufacturer's instruction. RNA integrity was assessed using Nanodrop One (Thermofisher Scientific). For RNA-sequencing the purity of RNA was further evaluated using AGRF's 2100 Bioanalyzer (Agilent). Samples with an RNA Integrity number (RIN) of 10/10 were used for library preparation. RNA libraries were prepared for sequencing using Illumina's Ribo-zero Gold protocol