Microbiota analysis of Mus musculus: caecal digesta
Microbiota analysis of caecal digesta were carried out at the UAB Microbiome Core facility. DNA was extracted from caecal content using a commercially available kit following manufacturers specifications. Unique barcoded primers were utilized to amplify DNA coding for the V4 region of the 16S rRNA gene using  PCR and isolated PCR products were purified by QIAquick Gel Extraction Kit.