GSM5257605: Ovary_Input_N; Oikopleura dioica; ChIP-Seq
ChIP was performed according to (Navratilova et al. 2017), finalized by library preparation using NEBNext Ultra II. Histone modification antibodies as listed in the Supplemental Table 1 were used. Following ChIP-seq, fragments were purified using Phenol-chlorophorm extractions and ethanol precipitation followed by Qiagen MinElute PCR Purification Kit. NEBNext UltraII Library preparation kit was used for library preparation. The number of cycles for final amplification varied between 8-15 cycles. Fragment sizes of barcoded libraries were assessed using Bioanalyzer libraries were purified using AMPure beads before pooling and sequencing. Pooled libraries were sequenced in a 2x75bp Paired-End format on HiSeq2500/4000 or HiSeq X-ten platform yielding about 50M reads per sample.  Illumina (NEBnext UltraII library preparation kit)