GSM4985032: Donor-3 Drop-seq; Homo sapiens; RNA-Seq
Single cells encpsulated with the nano-bead was lysed to release its content. The mRNA hybridizes to the DNA tails of the beads. After cell lysis, cDNA synthesis was carried out (Maxima Reverse Transcriptase, Thermo Fisher), followed by PCR (2X Kapa Hotstart Ready mix, VWR, 15 cycles). cDNA libraries were tagmented and PCR amplified (Nextera tagmentation kit, Illumina). Finally, libraries were pooled and sequenced (Illumina Nextseq 500, 20x55 bp).