GSM5930550: HuR [K120/182R] Input rep3; Homo sapiens; RNA-Seq
RNA was extracted with UltraPure Phenol:Chloroform:Isoamyl Alcohol and precipitated overnight in the presence of 100% ethanol, sodium acetate 3 M pH 5.5 and GlycoBlue Coprecipitant. The RNA pellet was washed with 70% ethanol, air dried and resuspended in H2O. The quantity and quality of the RNAs were evaluated using Agilent RNA 6000 Pico Chips (5067-1513, Agilent Technologies). 16-77 ng of RNA from each RIP sample and 250 ng of RNA from each input sample was used for RNA-seq library preparation. Sequencing libraries were prepared using the TruSeq Stranded Total RNA Human/Mouse/Rat kit (RS-122-2201, Illumina Inc.), following the manufacturer's guidelines.