GSM5289775: PShigh; Mus musculus; RNA-Seq
The single cell suspension was loaded onto a Chromium Chip A (10X Genomics PN 230027) and GEM generation, cDNA synthesis, and cDNA amplification. Library preparation of 10,000 cells proceeded using the Chromium Single Cell 5' Reagent Kit (10X Genomics PN 1000006) according to the manufacturer's protocol. cDNA amplification included 16 cycles and 11-50ng of the material was used to prepare sequencing libraries with 14-16 cycles of PCR. Indexed libraries were pooled equimolar and sequenced on a NovaSeq 6000 or NextSeq 500 in a 26bp or 28bp/91bp paired end run using the NovaSeq 6000 S1 or S2 Reagent Kit (100 cycles) or NextSeq 500/550 High Output Kit v2.5 (150 cycles) (Illumina). An average of 256 million paired reads was generated per sample. scRNA/TCR sequencing with feature barcoding