RNA-Seq of Andrographis alata
Total RNA, mRNA was purified by using poly-A enriched OligodT magnetic beads. Further, purified mRNA was fragmented with divalent cation and reverse-transcribed with Superscript II Reverse transcriptase by using random hexamers Second strand cDNAs synthesis was done in the presence of DNA polymerase 1 and cDNAs size selection was performed by using magnetic beads.