GSM5412710: Chicken preadipocytes - TCF21_IP_rep3; Gallus gallus; ChIP-Seq
Cells were then collected, treated with nuclear lysis buffer, and sonicated to mediate DNA fragmentation. The sonicated chromatin was used in each immunoprecipitation reactions with the anti-HA antibody (Abcam) or with control rabbit IgG (Cell Signaling Technology). Libraries were prepared according to Illumina's instructions. The brief procedures are end repair, adding 3' A tail, ligating adaptors and enriching/linearizing with PCR.