GSM5839162: Control1_Male_placenta; Mus musculus; RNA-Seq
On gestational day 18, animals were anesthized using isoflurane vapor.  Placentas were removed and the nondecidual portion was microdissected, flash frozen in liquid nitrogen, and stored at -80 degrees Celcius. RNA was isolated using the Qiagen RNeasy Mini Kit. RNA quantity was assessed using an Agilent TapeStation RNA assay.  Total RNA concentration was quantified via Qubit Fluorometric assay and subsequently all samples were normalized to an equivalent starting concentration. Sequencing libraries were prepared using the TruSeq Stranded Total RNA with Ribo-Zero Library Preparation kit (Illumina; San Diego, CA). Each sample was uniquely indexed (barcoded) to allow for pooling of all samples in a single sequencing run. Library size and quality were then assessed with an Agilent TapeStation D1000 DNA assay. Samples were normalized to ~4nM and pooled equally. Sequencing was performed on an Illumina NovaSeq S4 Flow Cell, XP running with a 150 cycle, paired‐end (2x150) sequencing run to generate approximately 50 million read pairs per sample.