Genome sequencing of wAlbB-II Wolbachia variant
1 g whole-genome amplified (MDA) gDNA was sheared into ~8Kb by using Covaris g-TUBE (Covaris 50079) with 1-hour incubation with 10 U T7 Endonuclease I (M0302S, NEB) at 37oC, followed by purification and size selection by Ampure XP beads (Beckman Coulter A63881). Oxford Nanopore Technology (ONT) sequencing library preparation was then carried out with Ligation Sequencing Kit (SQK-LSK109, ONT) along with Native Barcoding Expansion (EXP-NBD196, ONT). The barcoded DNA was purified using AMPure XP beads.  The barcoded sample was pooled with other samples to give 700 ng DNA in total and then ligated to the ONT Sequencing Adapter (AMII) using NEBNext Quick Ligation Module (E6056, NEB). The purified pool was then loaded onto a MinION Flow Cell (R9.4.1, FLO-MIN106, ONT) and sequenced for 72 hours using a GridION (ONT) controlled by MinKNOW software, version 20.06.9 (ONT). Base-calling and demultiplexing were performed within MinKNOW using Guppy version 4.0.11.