GSM4986348: Serum ctDNA methylation in rats with 10 Gy X-ray to lung; Rattus norvegicus; Bisulfite-Seq
Briefly, genomic DNA was extracted using DNeasy Mini Prep Kit (Qiagen) and ctDNA was extracted using the QIAamp Circulating Nucleic Acid Kit (QIAGEN) according to the manufacturer's instructions. Extracted DNA was quantified using a Qubit 3.0 Fluoromter (Thermo Fisher Scientific), and mixed with 0.05% artificially-synthesized unmethylated sequences, followed by sodium bisulfite conversion. Bisulfite conversion was performed using a EZ-DNA-Methylation-GOLD kit (Zymo Research). Then, Accel-NGS Methy-Seq DNA library kit (Swift Bioscience) was used to prepare the sequencing libraries. Libraries were qualified using 2100 bioanalyzer (Agilent) and sequenced on NovaSeq 6000 sequencer (Illumina) with 150bp paired end mode according to the manufacturer's instructions.