GSM6084373: RNAseq in Trim71(R595H) mESCs 1; Mus musculus; RNA-Seq
Total RNA were isolated from mESCs via Trizol reagent The Trim71(R783H) CLIP-seq was performed using the previously established HITS-CLIP protocol (Moore et al., 2014). The cut&run was performed using the CUT&RUN Assay Kit (Cell Signaling Technology, Cat#86652) according to its instruction manual. CLIP-seq, RNA-seq, and Cut&Run