GSM5026073: BSPCR_lib2_ZF108-SLN_T2-12-42_pos; Arabidopsis thaliana; OTHER
DNA was extracted with the CTAB protocol The DNA was bisulfite treated using the Epitect Bisulfite Conversion kit (QIAGEN). The conversion reaction in the thermocycler was performed twice to increase the efficiency. The converted DNA was amplified with the primers indicated in the manuscript of this study, using the Pfu Turbo Cx DNA polymerase (Agilent). Different PCR products for the same sample were pooled and purified with AMPure beads (Beckman Coulter). Libraries were generated using the Kapa HyperPrep DNA kit (KR0961) and the TruSeq DNA UD adapters (IDT for Illumina).