RNA-Seq of Callogorgia delta exposed to WAF oil, biological replicate 3
Total RNA was isolated using a Qiagen RNeasy Kit or a modified Trizol/Qiagen RNeasy protocol. Library preparations, including a poly-A selection step to target primarily eukaryotic (coral host) RNAs, were performed by the sequencing facilities on high quality (RIN values > 7) RNA samples (Illumina Truseq RNA library kit). Experimental exposure samples were further multiplexed and sequenced across Illumina HiSeq2500 rapid flow cells at the Fox Chase Cancer Center (FCCC, Philadelphia, PA) sequencing facility to acquire 100 bp single-end reads at approximately 20-30 million reads per sample.