GSM4987516: RF_pCF10_0; Enterococcus faecalis; RNA-Seq
Total RNA was extracted using a Qiagen RNeasy kit.  Ribosomal RNA was depleted using a MicrobeExpress kit.  All RNA samples were treated with Turbo DNase (ThermoFisher, rigorous method described by the manufacturer) and submitted to the University of Minnesota Genomics Center for Illumina library prep. Whole-cell RNA samples were prepared using a non-stranded TruSeq RNAv2 kit, and libraries were size selected to generate inserts of 200 bp. All samples were sequenced as paired-end reads on an Illumina HiSeq 2500 in high output mode (125 bp read length for whole-cell samples).