GSM5257801: YR3; Oncorhynchus mykiss; ncRNA-Seq
The samples were collected immediately, then flash-frozen in liquid nitrogen,and total RNA was extracted using Trizol reagent. Total RNA of the six libraries from dorsal skin was extracted using Trizol reagen kit (Invitrogen, Carlsbad, CA, USA), then purified RNA were ligated with 3' and 5' adapters. small RNA libraries were prepared for sequencing using standard Illumina protocols cDNA library were generated after the ligation products were reverse transcribed by PCR amplification and sequenced using Illumina HiSeq Xten by Gene Denovo Biotechnology Co. (Guangzhou, China).