GSM5255908: RAD21TEV depleted, +BDNF 30 minutes Rep2; Mus musculus; RNA-Seq
RNA was purified using the PicoPure RNA isolation kit (ThermoFisher) following the manufacturer's instructions. 200ng of total RNA was used to prepare libraries using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (polyA enrichment), following the manufacturer recommendations. Library quality and quantity were assessed on a Bionalayser and Qubit respectively. Libraries were sequenced on an Illumina Hiseq2500 (v4 chemistry) and at least 40million paired end 100bp reads per sample were generated per library.