GSM5839481: E16 3 base; Mus musculus; RNA-Seq
spiral ganglia (SG) were roughly dissected out of the                     cochlea with some sensory epithelia and mesenchyme still attached. At E16 SG                     were separated into basal and apical portions by cutting through the middle                     turn. At E14, E16 and E18, three biological replicates were collected containing                     pooled tissue from sperate litters. At P1, two biological replicates were                     collected. Dissociation at E14 and E16: Pooled SG were dissociated for 14                     minutes in 0.05% Trypsin-EDTA at 37°C, with two vigorous triturations. Trypsin                     was neutralized with the addition of an equal volume of FBS. Dissociation at E18                     and P1: Pooled SG were dissociated for 25 minutes in Papain (20U/mL) at 37°C,                     with triturations every 5 mins. Papain was neutralized with the addition of                     Ovomucoid inhibitor. Dissociated cells were passed through a 20µm strainer, spun                     down at 300 x g and then resuspended in PBS with 0.4mg/mL BSA and 0.2U/mL RNase                     inhibitor at a density of 1,100 cells/ml The single-cell suspension was loaded                     onto the droplet-based single-cell barcoding system (10x Chromium Controller,                     10x Genomics) and a Chromium NextGEM Single Cell 3' Reagent Kit v3.1 (10x                     Genomics) was used to prepare single-cell, barcoded 3' cDNA libraries according                     to the manufacturer's instructions. The libraries were sequenced on a                     NextSeq2000 instrument (Illumina). scRNA-Seq