RNA-sequencing of NCTC122 with phiX174 infection
5 mL samples of LB containing PhiX174 infected and uninfected NCTC122 Escherichia coli were collected at infection and at pre-lysis time points. These were treated with QIAGEN bacterial RNAprotect reagent. Total RNA purified from QIAGEN microbe kit with DNAse digestion. rRNA was digested using Ribo-Zero kit by Macrogen, and remaining RNA was sequenced in a stranded manner at 100bpPE.