GSM5661495: 3dps_Tgfb1_70_S15; Mus musculus; RNA-Seq
Culture plates were placed on ice for the extraction procedure. Cells were washed with 1ml of cold 1xPBS, suspended in 350µl of RLT-plus lysis buffer (Qiagen) and the total RNA was exctracted using RNEasy Plus Mini Kit (Qiagen). NEBNext Ultra II Directional RNA library after polyA purification