GSM5257956: MDA-MB-468 control - rep2; Homo sapiens; RNA-Seq
Total RNA was isolated from all cell lines (n = 3 per cell type) before and 48 hours after treatment of 20mM-NAM, using TRIzol® RNA Isolation Reagent (Invitrogen), and purified according to the manufacturer's instructions. The RNA concentration was determined using a NanoDrop ND-1000 spectrometer (Thermo Fisher, Waltham, MA), and the RNA integrity number (RIN) for each RNA sample was analyzed using a 2100 Bioanalyzer and the Agilent RNA 6000 Nano Kit (Agilent, Santa Clara, CA). The isolated total RNA was processed for preparing RNA sequencing library using TruSeq stranded total RNA sample preparation kit (Illumina, San Diego, CA) according to the manufacturer's instructions.