GSM5026120: EMseq_lambda_DNA; Escherichia virus Lambda; OTHER
The DNA was purchased from NEB (N3011) and methylated in vitro with M.SssI (NEB Catalog # M0226). The reaction was set up according to NEB's protocol for methylation of genomic DNA with their suggested adjustments to push methylation to completion (4 hours at  37°C). The library was generated with NEBNext Enzymatic Methyl-seq (EM-seq) (NEB Catalog # E7120), following manufacturer's instructions.