MISeqv2 of Leaf fungal endophyte: Adult Switchgrass
Leaves from three randomly chosen mature plants were harvested at each field block (four adult plant replicates). plant samples were surface sterilized by placing material (either seed or leaf) in a tissue cassette and submerging the sample in a series of 1min in nanopure water, 1 minute in 70% ethanol, 5 min in 10% bleach, and 1 min in 90% ethanol [40]. Plant material was extracted using Plant DNeasy kit with standard manufacture protocol and an extended lyses step. We amplified the ITS2 region using the fungal specific primers ITS9 (GAACGCAGCRAAIIGYGA; [41]) and ITS4 (TCCTCCGCTTATTGATATGC [42]).