Female_Recol_25C_2_3
hypervariable regions V1 and V2 of bacterial 16S rRNA genes were amplified. PCRs were conducted (98 C30 s, 30  [98 C9s, 55 C60s, 72 C90s], 72 C10 min). Sequencing was performed with v3 chemistry (2  300 cycle kit).