GSM6307678: ERI4, run3, Eribulin treated, 4 rounds, snRNA, multiome; Mus musculus; RNA-Seq
Single cells were captured using 10x Genomics Chip G (3' v3 samples) or Chip J (Multiome samples) Libraries were constructed following either the 10x Genomics 3' v3, or Multiome protocol 3' RNA/Multiome RNA: 28bp R1 (cell barcode + UMI), 98bp R2 (transcript); Multiome ATAC: 50bp R1 (insert), 50bp R2 (insert), 8bp I1 (index), 24bp I2 (cell barcode, includes 8bp dark cycles using custom recipe per 10x instructions). ERI1/ERI2/ERI3 and PAC1/PAC2/PAC3 samples from Run2 were multiplexed using Hashtag Oligos (HTO). HTO samplesheet is merged with feature reference file [lentiviral_barcodes_feature_reference_RNA_withHTObarcodes_forRun2.txt.gz]. Briefly, BC1 (GGAGAAGA), BC2 (CCACAATG) and BC3 (TGAGACCT) from 5P directional in Read2 represent ERI1, ERI2, and ERI3 respectively. Similarly, BC4 (GCACACGC), BC5 (AGAGAGAG) and BC6 (TCACAGCA) from 5P directional in Read2 represent PAC1, PAC2, and PAC3 respectively. 10x 3' RNA v3; 10x Mutliome RNA + ATAC