An ex vivo screening of selected glycans across multiple individuals
Incubated samples were removed from plates and added into Qiagens DNeasy PowerSoil extraction plates. Extraction plates were shipped on dry ice to CoreBiome, Inc for downstream extraction and library preparation. Extracts were quantified using the Quant-iT PicoGreen dsDNA assay (Thermo Fisher). Libraries were prepared using the NexteraXT kit and a HiSeq 1 x 150-cycle v3 kit (Illumina) was used to sequence samples.