GSM4986915: MY03; Homo sapiens; RNA-Seq
Total RNA was isolated using TRIzol reagent (Life Technologies) according to the instructions of the manufacturer, followed by purification using the RNA Clean-Up and Concentration Kit (Norgen Biotek). Ribosomal RNA-depleted sequencing libraries were constructed using the TruSeq Stranded Total RNA Sample Prep Kit with Ribo-Zero Gold (Illumina). Sequencing was performed using the Illumina HiSeq 2500 system and HiSeq version 3 sequencing reagents to generate 50 bp single-end reads.