GSM5412144: Sample 103119 from ADM D0; Homo sapiens; RNA-Seq
Total RNA was extracted from Trizol lysate using Qiagen miRNesay Mini Kit The preparation was performed using the reagents provided in the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760S, New England Biolabs,USA), in conjunction with the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) and the NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs, NEB #6440s)