GSM5024918: PF H3 ChIP; Trypanosoma brucei; ChIP-Seq
MNase digestion of permeabilised cells:  2.5 x 10^8 ccells/sample were harvested, permeabilised with digitonin and digested with 4U MNase (Worthington). 200 µl input DNA removed and 10 µl antibody added to each sample, incubated and antibody-protein-DNA complex collected using Dynabeads and magnetic pull down. DNA repair, adapter ligation, and library preparation were performed using the Illumina Truseq Chip-seq kit. The Illumina paired-end adaptor was ligated to the nucleosomal DNA. Indices were added to the DNA during PCR according to Illumina kit instructions. DNA was sequenced with an Illumina HiSeq 2500 platform using one flow cell, producing 2x 125 nt sequences