GSM4804800: NS_008; Mus musculus; RNA-Seq
A piece of skin tissue was cut from each mouse in the WO group  (NC-1, NC-2, NC-3, NC-4). One normal tissue and three skin lesion tissues were cut from each mouse in the UVR group, and four tissues were divided into groups according to the diagnostic criteria for skin squamous cell carcinoma (NC, AK-1, AK-2, cSCC). The RNAs were extracted using TRIZOL reagent (Sigma, USA). CDNA libraries were prepared by Nextera sample preparation kit (Illumina, USA) according to the instructions. Fragments of 300-450bp length were recovered using Ampure XP Beads (Beckman Coulter, USA). All libraries were quantified using 2100 Bioanalyzer and pooled as 1:1 at 2nM for sequencing in HiSeq 150 pair-end reads (Illumina, USA).