Illumina HiSeq 2500 sequencing; RNA-seq comparing WT to HDAC3 knock-out clones in naïve mESCs, as well as SL and EpiLC differentiation conditions at two distinct time points (1 and 2 days)
RNA-seq comparing WT to HDAC3 knock-out clones in naïve mESCs, as well as SL and EpiLC differentiation conditions at two distinct time points (1 and 2 days)
total RNA collected Cells grown in 2iLIF on gelatin coated plates and transferred to SerumLIF medium on gelatin for 48h transfected with gRNAs AGTTCTGCTCCCGTTACAC and TGAAACCCTGCATATTGGT, mutant clone RNeasy Mini Kit with on-column DNase digest (Qiagen) TruSeq Illumina mRNA-seq (stranded - high input)