GSM5155194: shDouble_EtOH_INPUT; Homo sapiens; ChIP-Seq
Cells were crosslinked, lysates were sonicated, precleared, and incubated with 10ug antibody (AR N-20) and agarose beads. Aliquots of precleared lysates were taken as input controls before antibody incubation. Beads were extensivley washed and protein-antibody complexes were eluted and reverse cross-linked. Supernatants were treated with Proteinase K, RNAase A. DNA was purified with the Wizard SV Gel and PCR Clean-Up System (Promega) and meausured with the Qubit dsDNA HS kit (Invitrogen). 1 ng of ChIP or input DNA per sample was used for library construction following NuGEN DNA-Seq library preperpation instructions.