amplicon sequencing of bacteria from culture enrichment (Plate count agar): replication 3
Genomic DNA were extracted from enrichment cultures of forest soil taken plate count agar. The V3 - V4 region of bacterial 16S rRNA was amplified using the forward primer BAC341F (5-CCTACGGGNGGCWGCAG -3)  and the reverse primer BAC785R (3- GACTACHVGGGTATCTAAKCC -5)