GSM5506605: Dam_Ctrl_For_Ets21C_4; Drosophila melanogaster; OTHER
DNA extraction: Midguts were homogenized in TENS buffer and gDNA was extracted using phenol-chloroform. gDNA was digested with DpnI, DamID adaptors ligated, digested with DpnII and amplified via PCR. DNA was sonicated to an average size of 350bp using a Covaris-S2 then digested with Sau3AI to remove the adaptors before processing with TruSeq DNA PCR-Free Sample Preparation kit (Ilumina, FC-121-3001), following its protocol.