GSM5851812: Cdk9in_D_1; Mus musculus; RNA-Seq
Zygotes were collected at 17h post hCG injection and cultured with or without BAY1251152 (Cdk9in or Noinject), or subjected to Trim-Away for SPT5 (Spt5KD), NELF-E (NelfEKD) or IgG. Embryos were cultured until 46h post hCG, washed in PBS and flash-frozen in lysis buffer. Libraries for single embryo RNA-seq were prepared with Smart-Seq2 protocol (PMID: 24385147). The quality of the libraries was assessed using a 2100 Bioanalyzer (Agilent). Samples were paired-end sequenced at PE150 on an Illumina NovaSeq 6000 platform.