GSM4984857: IP IgG Replicate 4 [396 2020]; Mus musculus; RIP-Seq
Protein extract was obtained from  cells with cold RIPA lysis buffer supplemented with a protease and phosphatase inhibitor cocktail. RNA was isolated and purified from mouse tissue and cells using RNAspin Miki Kit (Quiagen). Poly A purification (New England Biolabs, catalog # E7490) was the only performance of Input samples in only one step of purification. Library generation was done with the kit NEBNext Ultra II RNA Library Prep Kit (New England Biolabs, catalog # E7770), following manufacturer´s instructions and 12/18 cycles of amplification.