GSM5155456: LNCaP ENZ 48hrs  [scRNA-seq]; Homo sapiens; RNA-Seq
LNCaP parental (treated for 48 hours with enzalutamide or DMSO), RES-A, and, RES-B cells were harvested with 0.05% Trypsin-EDTA (Sigma T3924). After neutralization with complete medium, centrifugation (300 x g for 5 min), and resuspension in PBS/0.5% BSA, the cells were filtered through a 35 µm Cell Strainer (Corning 352235) and a single-cell suspension of living cells was acquired through sorting on a FACS Aria II cell sorter. Chromium Single Cell 3' Library, Gel Bead & Multiplex Kit, and Chip Kit (v3, 10x Genomics)