Illumina NovaSeq 6000 sequencing; small RNA sequencing from plasma samples of SARS-CoV-2 infected patients
small RNA sequencing from plasma samples of SARS-CoV-2 infected patients
Plasma samples were obtained from peripheral blood extracted in EDTA tubes after centrifugation.  Total RNA, including small RNAs, was isolated from 400Î¼l of plasma with the miRNeasy Serum Plasma Advanced kit (Qiagen). RNA quality and quantity were evaluated by the Bioanalyzer 2100 with Agilent RNA 6000 Nano Kit.  Small RNA libraries were constructed using the NEBNext Multiplex Small RNA Library Prep for Illumina.