MISeqv2 of Rain fungi: GLBRC Marshall  Scale Up
Rain collectors were left in the field for the full 51 days of the experiment with the samples collected within 6 hours of each rain event (15 rain events in total). Rain was brought back to the lab where 350 mL of rain was vacuum filtered through Supor polyethersulfone 0.2 m 47 mm filters (Pall, Port Washington, NY) . We extracted DNA using a PowerWater kit according to manufacturer protocol. We amplified the ITS2 region using the fungal specific primers ITS9 (GAACGCAGCRAAIIGYGA; [41]) and ITS4 (TCCTCCGCTTATTGATATGC [42]).