GSM4987004: IF5; Mus musculus; RNA-Seq
mice anesthetized with Ketamine/ Xylazine injection,  then brain microdissection in cold PBS, snap freeze in ethanol+CO2. stored at -80C until use. On day of experiment, tissue digested to release single nucleus suspension, then immunostained against Neun, and FACS sorted to obtain Neun negative nuclei. Immediately proceeded to 10x chromium protocol, barcoding and cDNA preparation. cDNA stored at -30C until all samples collected. Libraries prepared from all samples at the same time. 10X chromium kit following manufacturer's instructions