GSM5387773: primary mammary organoids - 1.5 uM CHIR99021 - rep1 ("CHIR_1.5uM_med"); Mus musculus; RNA-Seq
RNA was extracted using Trizol and RNA was extracted using the Qiagen RNeasy column purification kit. RNA quality was checked with a Bioanalyer (Agilent). PolyA+ stranded RNA library preparation was performed using the Illumina TruSeq stranded RNA prep kit according to the manufacturer's protocol. RNA libraries were prepared for sequencing using standard Illumina protocols. RNA-sequencing was performed on a HiSeq 2500 (Illumina) System using a stranded protocol.