GSM4862211: CD4-0789; Homo sapiens; RNA-Seq
T cells were lysed using RNAqueous lysis buffer (Invitrogen), lysates purified from genomic DNA using gDNA Eliminator Columns (Qiagen),  and RNA extracted using RNAqueous Micro Total RNA Isolation Kit (Invitrogen) cDNA was generated from total RNA using SMARTer Ultra Low Input RNA Kit for Sequencing (Takara Bio).  RNA-seq libraries were generated from by Nextera XT Library Prep Kit (Illumina)