GSM5930898: AD3030_2; Mus musculus; ATAC-seq
A total of 100,000 cells were lysed in lysis buffer (10 mM Tris-HCl pH 7.4, 3 mM MgCl2, 0.1% (v/v) IGPAL CA-630, 10 mM NaCl) for 20 min on ice. The nuclei were subjected to transposition with Tn5 transposase (TD501-01, Vazyme) for 30 min at 37°C. After tagmentation, DNA was purified with VAHTS DNA Clean Beads (N41101, Vazyme) for final library construction and high-throughput sequencing on the Illumina NovaSeq platform.