GSM5174034: miRNA-TDBPP-rep2; Danio rerio; miRNA-Seq
FRC-exposed embryos were homogenzied in RNAzol (4 replicates per treatment) and total RNA was extracted using the Direct-zol MiniPrep kit. Total RNA was then processed for library preparation mRNA and small RNA libraries were prepared using the PrepX™ mRNA and Illumina TruSeq Small RNA library kit respectively. Illumina HiSeq 3000, single end, 100 bp for mRNA and 50 bp for small RNA