GSM5386988: case6; Homo sapiens; miRNA-Seq
Peripheral blood samples from individuals were collected in EDTA tubes and centrifugated at 3,000 ×g for 15 min at 4 °C to collect the plasma. The sEVs were isolated by size exclusion chromatography (Exosupur columns, Echobiotech, China) and ultrafiltration (100 kDa molecular weight cut-off Amicon Ultra spin filters, Merck, Germany) methods. Total RNA was extracted and purified from plasma exosome using miRNeasy Serum/Plasma Advanced Kit (Qiagen, cat. No. 217204) Small RNA libraries were prepared for sequencing using the SMARTer Stranded Total RNA-Seq Kit (Takara Bio USA, Inc.)