GSM5025655: wt 3; Synechocystis sp. PCC 6803; RNA-Seq
For RNA extraction, the TRIzol Reagent (Ambion) was used in combination with the  PurelinkTM RNA Mini Kit (Ambion). Briefly, cells were disrupted in TRIzol containing 0.2   g of 0.2 mm-diameter glass beads (acid washed, Sigma) using a FastPrepH-24 (MP  Biomedicals) and the following extraction steps were performed according to the  manufacturer's instructions. DNase treatment was performed according to the On-column  PureLink® DNase Treatment Protocol (Life Technologies/Invitrogen). RNA was quantified  on a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Inc.) and the quality  and integrity was checked using the ExperionTM RNA StdSens Analysis Kit (Bio-Rad). RNA libraries were prepared for sequencing using standard Illumina protocols