GSM5951792: Cardiac_Sampl36; Homo sapiens; RNA-Seq
Samples were prepared with the Direct-zol RNA Microprep w/ Zymo-Spin IC Columns (R2060, Zymo Research) and sequenced. Each replicate had an average of 24.4 million 150 bp long paired-end reads. FastQC v0.11.2 was used for sequencing quality assessment. Reads were then aligned to the GRCh38(hg38) reference genome using STAR v2.5.3a with splice junctions being defined in GTF file (obtained from GRCh38). An average of 85.3% of reads was aligned to the reference transcriptome.