whole exome
The qualified genomic DNA samples were randomly fragmented by Covaris and the size of the library fragments was distributed predominantly between 150 and 200 bp. Adapters were then ligated to both ends of the resulting fragments. The adapterligated templates were purified by the Agencourt AMPure SPRI beads, and fragments with insert size about 176 bp were excised. Extracted DNA was amplified by ligationmediated PCR (LMPCR), purified, and hybridized to the SureSelect Biotinylated RNA Library (BAITS) for enrichment