miRNA-seq of tissue: adult female rat ovary
The procedure was performed according to the standard steps provided by Illumina, Inc., including library preparation and sequencing experiments. Small RNA sequencing libraries were prepared using TruSeq Small RNA Sample Preparation Kit (Illumina, San Diego, USA). After the preparation of each library, the constructed libraries were sequenced using Illumina Hiseq2000/2500 with a read length of 1x50 bp. Bioinformatics analysis was performed using the miRNA data analysis software ACGT101miR provided by LC Sciences.