mucH-2
B04Sm5, mucG, and mucH were grown in BHI at 37C in 5% CO2 / 95% air to mid-log phase (OD600 ~0.65 for B04Sm5 and mucH; and 0.25 for mucG, because of the growth defect phenotype).  1 ml aliquots of B04Sm5 and mucH and 50 mL aliquots of mucG were pelleted and frozen at -80 C. Total RNA was extracted using a RNeasy Power Microbiome Kit (Qiagen, Inc.) according to the manufacturers instructions.  rRNA was depleted using a NEBNext rRNA Depletion Kit (Bacteria) (New England Biolabs) according to the manufacturers instructions.  polyA tails were then added to the rRNA-depleted RNA using E. coli Poly(A) Polymerase (New England Biolabs).  RNA was checked for quality at each step using a Qubit (Thermo Fisher Scientific) and a TapeStation (Agilent Technologies).  RNA libraries were then constructed using the Direct cDNA Sequencing Kit (Oxford Nanopore Technologies) and sequenced on a GridION using a R9.4.1 Flow Cell (Oxford Nanopore Technologies).  Basecalling was performed using Guppy v4.0.11/MinKNOW v20.06.09.