GSM6360822: S1-02; Canis lupus familiaris; RNA-Seq
Two 50 micron thickness sections were obtained from each FFPE tumor sample and processed for total RNA extraction using an Qiagen RNeasy FFPE kit. One microgram of total RNA was enriched for mRNA using Ribominus Eukaryotic V2. Sequencing libraries were constructed using 200 nanograms of enriched mRNA and the Ion Total RNA-Seq Kit V2 without further fragmentation while all purification and size selection were performed using AMPureXP beads.