RNA-Seq of Knufia petricola: pooled growth conditions
K. petricola A95 was grown in nine different cultivation conditions (biofilm grown for 14 d on MEA at 25 C, suspension cultures grown for 16 h, 4 d and 8 d in MEB at 25 C, for 4 d in MEB plus 0.5% carboxymethylcellulose at 25 C, for 4 h in ASM pH 5 at 25 C, 16 C and 30 C, and for 7 d in ASM pH 3.5). RNA was extracted using the RNeasy Midi Kit; cellular lysis was achieved in metal beakers with glass pearls by vigorous shaking (10 min at 30 Hz) in a Retsch MM 400 Mixer Mill. RNA from the resulting supernatant was isolated by column purification. Quality and purity of RNA was determined using a 2100 Bioanalyzer Instrument. Library preparation and sequencing was performed at Fasteris SA (Plan-les-Ouates, Switzerland) on one lane of an Illumina HiSeq 2000 with chemistry v3.0 and with 100 bp paired-end reads.