GSM5951826: E104 rhlR- rep3; Pseudomonas aeruginosa; RNA-Seq
mRNA was preserved immediately upon cell harvest using Qiagen RNAprotect. Cells were lysed using a bead-beating protocol and mRNA was extracted using Qiagen RNEasy miniprep columns, followed by DNAse I treatment and cleanup using another round of RNEasy-based purification. rRNA was removed using Illumina RiboZero protocol. 150bp PE Illumina TruSeq