GSM5025563: IVA262A1A; Mus musculus; RNA-Seq
The embryos were isloted and  incubated separately in a solution of papain  for 11 min at 37ºC in presence of 10ug/ml of both DNAse  and Rock Inhibitor. The papain solution was then removed, and mechanical dissociation was performed through pipetting medium. Library generation for 10x Genomics analysis were performed following the Chromium Single Cell 3′ Reagents Kits (10x Genomics) and sequenced on an Hiseq4000 (Illumina).