GSM5661447: ATACseq_NPC_5088_WT; Mus musculus; ATAC-seq
ATAC-Seq: 50,000 to 100,000 nuclei from GE_NPC were used for ATAC-seq library preparation. ATAC-seq library preparation using reagents from TruePrep DNA Library Preparation Kit V2 (Vazyme, TD501-02, China) following the manufactory's protocol with modifications. RNA-seq: Total RNA was extracted from fresh frozen GE_NPCs and GE tissue at E15.5 by Direct-zolTM RNA MicroPrep kit (ZYMO RESEARCH, catalog #R2060) and RNeasy Lipid Tissue Mini Kit (Qiagen, catalog #74804), respectively. mRNA-seq libraries from GE_NPCs were prepared by BGI Genomics, China. Total RNA-seq libraries from GE tissue were prepared using QIAseq™ Stranded Total RNA Lib Kit (Qiagen, Cat#180745) following the manufacturer's instructions.