GSM5151343: s3b_HSCactivated_miR15a; Mus musculus; RNA-Seq
Total RNA from each sample was collected using TRIzol (Invitrogen) and quantified by nanodrop (Thermo Fisher). Only RNA samples with RNA integrity number (RIN) > 9 by Agilent 2100 bioanalyzer (Cat# G2939BA, Agilent Technologies) were used. RNA-Seq libraries were prepared by using TruSeq kit (Cat# RS-122-2001, Illumina) NextSeq genome sequencer (Cat# SY-415-1002, Illumina), 80nt, Single-End Read, 4 samples per lane