GSM4805449: 2nd restim TBBz rep1; Homo sapiens; RNA-Seq
Total RNA of isolated CAR T cells was isolated using NucleoSpin RNA XS kit (MACHEREY-NAGEL, Germany). The RNA quality was determined with the Agilent 4200 Tapestation (RIN value > 9). RNA sequencing libraries were constructed using Illumina Truseq Stranded mRNA LP kit. Briefly, mRNA was purified by oligo-dT beads and fragmented through an enzymatic reaction. After fragmentation, cDNA was generated through reverse transcription. The cDNA libraries were constructed and followed by 100 bp paired-end mode on DNBSEQ-400 platform. External RNA controls consortium (ERCC) RNA spike-in mixes (Thermo Fisher, 4456740) were included for quality assurance. The quality of all paired-end reads was analyzed using FastQC software, and the per base sequence quality of all sample was above Q30.