GSM5661537: Naive_S1; Mus musculus; RNA-Seq
Naive CD8+ T cells from spleen and lymph nodes were sorted using fluorescence-activated cell sorting (FACS). Cells were washed with PBS, suspended in RLT lysis buffer (Qiagen) and the total RNA was exctracted using RNEasy Mini Kit (Qiagen). RNA libraries were prepared for sequencing using standard Illumina protocols.