GSM5025727: E13.5 Zfp57-/- maternal-zygotic mutant (M-Z-) WGBS rep4; Mus musculus; Bisulfite-Seq
Live E13.5 embryo were flash frozen on liquid nitrogen, and genomic DNA was harvested using OMEGA TISSUE DNA Kit. DNA (1 µg) was spiked with 1 ng unmethylated lamda DNA (Promega), fragmented, end-repaired, A-tailed, and adapter-ligated using Truseq DNA sample prep kit (Illumina). Then purified DNA was treated by EZ-DNA Methylation-Gold Kit(Cat#D5006, Zymo Research, USA). After amplified with PfuTurbo Cx Hotstart DNA Polymerase(Cat#600410, Agilent, USA), DNA was cleaned with VAHTSTM DNA Clean Beads(Cat#N411-03, Vazyme, China).