monk parakeet male_testis-1 RNA-seq
The library fragments were purified with QIAQuick PCR kits (Qiagen) and eluted with Elution buffer. After terminal repair, poly(A) sequence and adapter were implemented. The resulting cDNA libraries were run on a 2% agarose gel and bands of approximately 250 bp were excised and used for pairedend (2  125 bp) sequencing on an Illumina HiSeq X ten platform by Annoroad Gene Technology Co. Ltd.