ITS seq of tea: BSW
An equal amount of DNA was used for PCR amplifications  with two sets of primers in the V4 variable regions for 16s rRNA and ITS1 region for fungi. Sequencing libraries were generated after PCR with equal amount using Illumina TruseqTM DNA Sample Preparation Kit (Illumina, San Diego, USA) following manufacturers recommendations. In order to select DNA fragments of preferentially ~400 bp in length, agarose electrophoresis was performed.  After purification using a spin column (QIAGEN, Dusseldorf, Germany), DNA fragments with ligated adapter molecules on both ends were selectively enriched using Illumina PCR Primer Cocktail in a 10 cycle PCR reaction. Products were purified using the AMPure XP system (Beckman Coulter, Beverly, USA) and quantified using the Agilent high sensitivity DNA assay on the Agilent Bioanalyzer 2100 system. Libraries were sequenced with the PE250 specification on the Hiseq 2500 platform (Illumina, San Diego, USA).