GSM5174215: RNAseq_3D7_wild-type_10-16h_rep1; Plasmodium falciparum 3D7; RNA-Seq
Cultured parasites were immediately pelleted and resuspended in TRIzol (ThermoFisher, Cat.N: 15596026). The samples were then processed using the RNeasy Mini Kit (QIAGEN, Cat.N: 74104) with DNase treatment, according to the manufacturer's instructions. Purified RNA was then used for library generation using the NEBNext UltraTM Directional RNA Library Prep Kit following the manufacturer's instructions.