WGS of E.coli: south american camelids from middle Germany
The strains were grown overnight at 37 C in 5 mL of Luria-Bertani broth (Carl Roth GmbH, Karlsruhe, Germany). The genomic DNA was purified using the DNeasy UltraClean Microbial Kit (Qiagen). The concentration of the DNA was determined using a NanoDrop One/OneC Microvolume UV-Vis Spectrophotometer (Thermofisher, Dreieich, Germany). DNA sequencing libraries were prepared, and paired-end sequencing  was performed by Eurofins Genomics Europe Sequencing (Illumina NovaSeq, 2 x 150 bp; Constance, Germany) or LGC Genomics ((Illumina NovaSeq, 2 x 250 bp; Berlin, Germany).