GSM5155159: JE_RNA_MV411_dTAG13_06h_R1; Homo sapiens; RNA-Seq
RNA was extracted from cells with the RNeasy Kit and on-column DNA digestion (Qiagen). For RNA-sequencing of MV4-11 cells, polyA mRNA was isolated and libraries were prepared using the TruSeq Stranded mRNA Kit (Illumina) according to the manufacturer's protocol. All samples were sequenced on a NextSeq500 instrument with paired-end 150bp reads to a depth of 40-60M reads/sample.