GSM5839378: HEK293T - PABPC RIP Rep3; Homo sapiens; RIP-Seq
RNA was isolated using a standard Trizol (Life Technologies) RNA extraction. RNA was DNAse treated before libraries were prepared for sequencing. cDNA libraries from three independent biological replicates were prepared from 1ug RNA using Illumina ribodepleted RNA stranded kit. Libraries were sequenced on the Illumina HiSeq 4000, single-end reads (75 nucleotides).