GSM5838126: Control, patient 5; Homo sapiens; RNA-Seq
Total RNA was extracted from cryosections of residual O.C.T.-embedded/fresh-frozen biopsy aliquots, using miRNeasy Mini Kit (Qiagen, Germantown, MD) and a QIAcube automated handler (Qiagen). cDNA libraries were prepared using TruSeq Stranded mRNA Sample Prep Kit (Illumina, San Diego, CA). Libraries were amplified on an Illumina CBot automated cluster generator and sequenced as 100 X 2 paired end reads on an Illumina HiSeq 4000.