GSM6310590: MCF-10A, 2; Homo sapiens; RNA-Seq
5x10^E6 cells were harvested for RNA-seq. RNA was extracted using the Qiagen RNeasy Plus Mini Kit. The RNA was run on an Agilent 2100 Bioanalyzer using the RNA 6000 Nano Chip to assess RNA quality and quantity.  1 µg of total RNA was used as input to the Illumina TruSeq Stranded Total RNA Library Prep Kit Ribo Zero Gold H/M/R.  Library size was assessed using the DNA 1000 chip on the Bioanalyzer, and the libraries were quantified using a Qubit fluorometer.  Pair-end sequencing was run on an Illumina NextSeq 500 instrument.  Four replicates were processed for treatment/conditions out of which three were biological replicates.