GSM6360726: Escherichia coli, Day 1, Control, rep 1; Escherichia coli; RNA-Seq
Total RNA was harvested using Rneasy mini  kit (Qiagen). Ribosomal RNA was removed from total RNA samples using either Ribo-Zero rRNA Removal Kit Bacteria (Illumina) or RiboMinus Bacteria 2.0 Transcriptome Isolation Kit following the manufacturer's instructions. Ethanol precipitation was used to purify depleted RNA. 100 ng of purified mRNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using TruSeq Stranded mRNA Library Prep Kit following manufacturer's protocols for purified mRNA input and Low Sample Protocol.