GSM5219939: 231-Kv-2; Homo sapiens; RNA-Seq
RNA was extracted from three separate biological replicates of MDA-MB-231-Control, 231-Kv1.5, and 231-Kir2.1 cells using the the RNA MiniPrep Kit (R1057; Zymo Research; Irvine, CA).  Samples were confirmed to have sufficient quality with Agilent Bioanalyzer before undergoing library preparation. Library preparation was done by Tufts University Genomics Core using the TruSeq stranded mRNA kit (20020594; Illumina; San Diego, CA). Samples were run on a HiSeq2500 platform at a depth of 50 million single-end 50 base pair reads to allow for transcript quantitation.