HiSeq X Ten paired end sequencing; RNA-seq and RRBS of developing zebrafish larval brain and WGBS of 4 week old WT and dnmt3aa/dnmt3ab CRISPR/CAS9 KO brain
RNA-seq and RRBS of developing zebrafish larval brain and WGBS of 4 week old WT and dnmt3aa/dnmt3ab CRISPR/CAS9 KO brain
Whole brains were dissected from zebrafish larvae and adults before being snap frozen in liquid nitrogen and stored at -80°C Genomic DNA (gDNA) was extracted from brains using the QIAGEN DNeasy Blood & Tissue Kit (QIAGEN, Chadstone, VIC, Australia) according to manufacturer instructions. For RNA extraction, half the lysate from the first step of the DNA extraction from the QIAGEN DNeasy Blood & Tissue Kit was added to TRIsure (Bioline) and purified following manufacturer instructions. All experiments in this study were performed in biological replicates. RNA-seq libraries were prepared with 1000ng of input RNA material using the KAPA mRNA HyperPrep Kit, according to the manufacturer's instructions.