GSM5505945: Donor RPE/choroid 1; Homo sapiens; RNA-Seq
The retinal pigment epithelial cells/choroid layers were dissected from donor retinas and processed for RNA extraction using the Rneasy kit with Dnase I (Qiagen). RNA quality was checked using bioanalyzer and transcriptome libraries were prepared using the TruSeq Stranded mRNA kit (Illumina) RNA libraries were prepared for sequencing using standard Illumina protocols