GSM5950109: Human peripheral blood mononuclear cells (PBMCs); Homo sapiens; RNA-Seq
Mice were anaesthetized with isoflurane. Brains were extracted and dissected in NMDG-ACSF. Tissue was digested with papain in trehalose-HEPES-ACSFand triturated to generate single cell suspensions. Human PBMC samples were commercially acquired from Hemacare. Sequencing libraries were constructed following 10x Genomics 3' v2 standard protocols. 3' Single-cell RNA-Seq