GSM5257983: 1 - nanoparticle positive tumour endothelial cells; Mus musculus; RNA-Seq
Tumours removed, mechanically and enzymatically digested into a single cell suspension. Tumour endothelial cells were enriched using Miltenyi MACS system by first depleating CD45+ cells, and then selection CD31+ cells. The enriched tumour endothelial cells were then sorted into nanoparticle positive and nanoparticle negative fractions using FACS. The isolated cells were then frozen at -80C in Trizol. RNA extraction done using the Qiagen RNeasy Plus Mini Kit. cDNA synthesis and amplification were done using the SMART-Seq v4 Ultra Low Input Kit for Sequencing from Clontech. Sequencing was done by 2x150bp paired end reads.