GSM5729597: Mouse brain tissue - CER - DEX13; Mus musculus; RNA-Seq
Brains were perfused, extracted and kept in -80oC until further use. The brain regions were extracted by punching of coronal sections following the Paxinos stereotaxic coordinates. RNA was extracted from each brain region of each animal using an automated Chemagic 360 instrument with an integrated dispenser and the chemagic RNA Tissue Kit (CMG-1212) in Paxgene tubes following manufacturer's instructions. Messenger RNA sequencing libraries were prepared using the QuantSeq 3' mRNA Fwd kit (Lexogen) following manufacturer's instructions with the addition of unique molecular identifiers (UMIs- UMI Second Strand Synthesis Module for QuantSeq FWD) for tagging of individual transcripts. Libraries were single-end sequenced on a whole Flow Cell of an Illumina HiSeq4000 sequencer using 75bp long reads for a total coverage of an average of 10M reads per library.