BRCA_RNA_seq_ctrl
mRNAs were separated by using oligo-dT beads and fragmented to 200-250 bp. After cDNA was synthesised, the ends were repaired for blunt ends and the 3 ends were adenylated. To the adenylated sites, adapters were linked and subsequently PCR amplification of the library was done.After the construction of the libraries, their concentrations and insert sizes were detected using Qubit and Agilent Tapestation, respectively. High throughput sequencing was performed using Illumina HiSeq2500 to obtain 100-bp paired-end reads.