GSM5025011: ZT14_INPUT; Drosophila melanogaster; ChIP-Seq
Fly heads were removed, flash frozen on dry ice. Nuclei were isolated and crosslinked followed by DNA sonication. The Protein-DNA complexes were immunoprecipitated and ChIPped DNA is eluted for library prep. DNA sequencing library construction was performed using NEBNext® Ultra™ DNA Library Prep Kit for Illumina (New England Biolabs) following manufacturer's instructions for end repair, adaptor ligation and size selection.