GSM5151848: SET-seq_10000_cells_Exp_rep2; Mus musculus; RNA-Seq
The total RNA was extracted by UNIQ-10 Column Total RNA Purification kit (Sangon Biotech). Cells were harvested and then lysed in NE buffer (20 mM HEPES pH 7.5, 0.5 mM KCl, 0.5 mM Spermidine, 0.5% Triton X-100, 20% Glycerol, 1mM PMSF) on ice for 10min. Bulk libraries preparation was described previously(Kaya-Okur et al., 2019a). Sc-libraries were constructed with a structure similar to True-seq libraries for pooling with other samples as described(Ai et al., 2019a).