GSM6265622: PoxCxrA-2; Penicillium oxalicum; RNA-Seq
Total RNA were extracted using TRIzol RNA Kit The total RNA is enriched, the RNA fragments are reverse transcribed with random N6 primers, and the cDNA obtained to synthesize double-stranded DNA. A sticky end of "A" is connected with a linker, the ligated product is amplified by PCR with specific primers, the PCR product is heat denatured to form a single-stranded DNA, and the single-stranded DNA is obtained by a bridge primer to obtain a single-stranded circularization library.