GSM5387491: DS4: E18.5 Nmnat1-/- Retina; Mus musculus; RNA-Seq
Retinas were removed, flash frozen on dry ice, and total RNA was harvested using Trizol reagent. Total RNA resuspended in DEPC water was sent to Macrogen Corp. (now Psomagen Corp., Rockville, MD, USA) for library construction and sequencing libraries were constructed by Psomagen Corp. using an Illumina TruSeq Stranded mRNA LT Sample Prep Kit (part# 15031047 Rev. E)