GSM5025909: Wild Type 1; Candida albicans; RNA-Seq
Cells were broken by hot phenol and RNA was purified using the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturers instructions. Samples were treated with TURBO DNase (Invitrogen GmbH, Darmstadt, Germany) to remove any residual genomic DNA, and remaining RNA was cleaned by a second purification using the RNeasy Mini kit. Further steps to prepare for and conduct RNA sequencing were done by Eurofins Genomics (Konstanz, Germany). Such steps included purification of poly(A) mRNA molecules, mRNA fragmentation, preparation of strand-specific cDNA libraries, adapter ligation, and Illumina (HiSeq 2500) sequencing (single reads, 1x 50 base pair length).