GSM5454512: QPCTL-deficient and proficient TMEs; Mus musculus; RNA-Seq
3 TMEs were harvested from QPCTL-KO mice innoculated with QPCTL-KO B16F10 tumor cells, and 3 TMEs were harvested from QPCTL-WT mice innoculated with QPCTL-WT B16F10 tumor cells. Single cell suspensions were made, and live cells were FACS sorted. Each sample was  stained with sample hashtags individually, and subsequently pooled into a single sample. Library preperation was performed in according the manufacturers protocol of the 10X genomics Chromium Single Cell 3' kit. Sequencing was performed on Next-Seq550 (Illumina) Library preperation was performed in according the manufacturer's protocol of the 10X Genomics Chromium Single Cell 3' kit (V3)