GSM4867731: Mox-2; Homo sapiens; RNA-Seq
Cells were then harvested in Buffer RLT (Qiagen) + 1% 2-Mercaptoethanol (Sigma-Aldrich) and RNA was extracted using Rneasy Mini kit (Qiagen) following manufacture's instructions. Illumina Truseq Stranded was use for mRNA library preparation kit according to the manufacturer's protocol (Illumina). Prepared libraries were sequenced on the Illumina Nextseq 500 at 3 million reads per sample at 2x75 cycles.