GSM5661352: Gard-1_rep2; Mus musculus; RNA-Seq
Bladders were removed, flash frozen in liquid nitrogen, RNA was extracted using RNeasy Plus Mini kit (Qiagen). ds-cDNA was prepared from 10ng total RNA using the SMARTer Ultra Low RNA kit for Illumina Sequencing (Takara-Clontech) RNA libraries were prepared for sequencing using standard Illumina protocols