GSM5839343: hMSNs-quin-cgs-r1; Homo sapiens; RNA-Seq
hMSNs were washed 1X in PBS. Total RNA was isolated using Direct-zol RNA MicroPrep Kit (Zymo Research) according to the manufacturer's protocol. RNA samples were collected in 2mL RNAse-free tubes and chilled on ice throughout the procedure. cDNA synthesis was performed using 1000 ng of total RNA and the iScript Reverse Transcription Kit (BIO-RAD) according to the manufacturer's protocol. qPCR reactions were performed using SsoAdvanced™ Universal SYBR® Green Supermix (BIO-RAD) on a BIORAD 384-well machine (CXF384) with custom designed using Primer3 RNA libraries were prepared for sequencing using standard Illumina protocols through Azenta and UNC HTSF