Control3
Total RNA was extracted from liquid B. cinerea cultures grown for 48 h in 1/2 PDB with  the addition of tobacco seedlings 5 days post germination (150 seedlings/ 50 mL media), mock or supplemented with 25 M 6BAP, 50 mg fungal mass per sample, using the  Norgen total RNA purification kit (Norgen Biotek corp.) according to the manufacturers  instructions. RNA yield and purity was measured by Nanodrop (ND-1000  Spectrophotometer, Wilmington, USA). RNA integrity was assessed by electrophoresing  on a 1.5% agarose gel. RNA quality was validated for by running on a Bioanalyzer 2200  Tape-Station (Agilent Technologies, California, USA). cDNA libraries were prepared for  sequencing using the TrueSeq RNA kit (Illumina, San Diego, CA, USA). Libraries were evaluated with Qbit and TapeStation (Agilent Technologies, California, USA). Sequencing libraries were constructed with barcodes for sample multiplexing. Pooled libraries of the 6 samples were sequenced on one lane of an Illumina Hiseq 2500  instrument using a 60-bp single-end RNA-Seq protocol, to obtain ~20 million reads per  sample. Sequencing was performed at the Weizmann Institute of Science, Israel.