RepSeq of human sorted immune subsets
Total RNA was isolated using the RNeasy mini kit (Qiagen) according to the manufacturers instructions. In all experiments described here, cDNA libraries were obtained using 5-RACE with unique molecular identifiers (UMI) (20) using Human TCR profiling kit (MiLaboratory LLC). Libraries were sequenced on Illumina NextSeq 500 using 300 cycle reagent kit, paired-end 150 + 150 nt mode. UMI was extracted and saved to R3 file (!!!)