NextSeq 500 paired end sequencing
extraction blank using water instead of ancient dental calculus for input, and built into libraries with an in-house protocol optomized for ancient DNA; https://dx.doi.org/10.17504/protocols.io.bidyka7w; https://dx.doi.org/10.17504/protocols.io.bmh6k39e; https://dx.doi.org/10.17504/protocols.io.bvt8n6rw; https://dx.doi.org/10.17504/protocols.io.beqkjduw