RNAseq of OCIAML3 treated with DMSO
Cells (1x10^6 in 1.5 ml growth media) were seeded in triplicate in 24-well deep round-bottom plates and treated with BRM011 or equal volume DMSO. BRM011 doses were chosen to induce >90% MYC down-regulation (30 nM HL-60, MOLM-13, HEL 92.1.7, Kasumi-1, CMK-86, OCI-AML-3; 200 nM SKM-1; 300 nM THP-1). After 24 hours, cells were harvested by centrifugation at 4C followed by cell lysis/RNA extraction using RNEasy plus mini kit (Qiagen) according to manufacturer's instructions. Samples were eluted in 30 l of water. RNA was quantified and assessed for purity using the RNA ScreenTape on the Agilent 4200 TapeStation System.