Tru-Seq of Pteropus vampyrus kidney, liver and lung
A total of 200 L of pooled materials from each set were extracted using Direct-zol RNA MiniPrep kit with TRI-Reagent (Zymo Research Corporation, Irvine, CA, USA) per manufacturers instruction. RNA was subjected to in-column DNase I (New England BioLabs Inc., Ipswich, MA, USA) digestion as instructed in the Direct-zol RNA MiniPrep manual. Extracted and DNase I digested RNA were treated with Ribo-Zero Gold Epidemiology Kit (San Diego, CA, USA) as described in the instruction manual. Sequencing cDNA libraries of ca. 260 bp were constructed using a TruSeq RNA Exome kit (Illumina; San Diego, CA, USA) following the manufacturers protocol. Library quality was assessed with an Agilent Bioanalyzer High Sensitivity DNA Assay and sequenced on an Illumina HiSeq2000 platform using a 76 bp paired-end run.