16S rRNA amplicon sequencing of organic forest soils (Quebec, Canada)
The 16S rRNA gene V5-V6 region was amplified by PCR using the primer pair 799F/1115R (Reysenbach and Pace, 1995; Chelius and Triplett, 2001). Amplicons were pooled into a single library which was sequenced on a 2 x 250 bps Illumina MiSeq run. Sequences (forward and reverse) were then demultiplexed into fastq files corresponding to each sample.