DNA-Seq of Pseudomonas fluorescens
The qualified DNA samples detected by electrophoresis were randomly broken into fragments of about 354bp length by Covaris ultrasonic crusher, and the whole library was prepared by terminal repair, A-tail, sequencing joint, purification and PCR amplification. After the construction of the library is completed, the library is initially quantified by Qubit2.0 and diluted to 2ng/uL, and then the insert size of the library is detected by Agiient2100. After insert size meets the expectations, the effective concentration of the library is accurately quantified by Q-PCR method to ensure the quality of the library.