PBA-BLITZ Transcriptome data
Total RNA extraction was performed using the RNeasy 96 Plant Kit (QIAGEN, Hilden, Germany) following the manufacturers instructions. The concentration and quality of RNA was confirmed using a NanoDropTM UV-Visible spectrophotometer (Thermo-Scientific, Wilmington, DE, USA) at the wavelength ratios of A260/230 and A260/280 nm. The integrity of extracted RNA samples was evaluated using TapeStation 2200 platform with RNA ScreenTape System (Agilent Technologies, Santa Clara, CA, USA), following manufacturers guidelines. RNA-Seq libraries were prepared using the Agilent SureSelect Strand Specific RNA Library Preparation Kit and evaluated on TapeStation 2200 platform with D1000 ScreenTape System (Agilent Technologies, Santa Clara, CA, USA). Each RNA-seq library was paired-end sequenced using Illumina Hiseq 2000 Sequencing platforms (Illumina Inc., San Diego, USA).