RNA-seq of Rhincalanus gigas CV juvenile
Total RNA was extracted from individual copepods using the Aurum Fatty and Fibrous Tissue Kit (Bio-Rad), without DNase treatment. Libraries were constructed  using the KAPA Stranded RNA-seq Library Preparation Kit. The barcoded libraries were sequenced with 150 base pair paired-end reads to a depth of 40 M reads (20 M pairs) on an Illumina HiSeq 4000.