mtDNA-seq of Oenothera elata ssp. hookeri strain johansen Standard: mature rosette leaves
A mate pair library was generated from mtDNA for paired end sequencing according to the protocol of the Nextera Mate Pair Library Prep Kit. Due to the limited input DNA amount of 1 g, the library was not additionally size-selected by e.g. Blue Pippin or SAGE Science. Sequencing-by-synthesis was performed on a HiSeq 3000 with 2x 150 bp paired-end reads.