metagenome of M. auratus: bile adult infected by O. felineus
Amplicons containing the V3-V4 hypervariable region of the 16S rRNA gene were obtained by PCR using standard gene-specific primers for the 16S rRNA gene with TrueSeq adapters. To isolate a desired DNA fragment and get rid of nonspecific products, amplicons were purified by size selection than subjected to a second PCR round where PCR products were barcoded (NEBNext Multiplex Oligos for Illumina Dual Index Primers Set 2, cat. #: E7780S, New England Biolabs, UK). Size selection of DNA was performed on Agencourt AMPure XP beads (cat. #: a63881, Beckman Coulter).