BL21-AI wildtype
Genomic DNA was obtained from cultures prepared in 2 ml Lysogeny Broth (LB) by purification using the QIAGEN DNeasy UltraClean Microbial Kit. Resulting samples were submitted to the UCR Genomic Core at the Institute for Integrative Genomic Biology for processing and sequencing. Samples were sheared using a Covaris S220 Ultrasonicator and libraries prepared using an NEBNext Ultra II Library Prep kit. After preparation, libraries were analyzed using qPCR and an Agilent 2100 Bioanalyzer