Illumina sequencing of Intestinicoccus colisanans: MH27-1
DNA was extracted using the Nextera DNA Flex Direct Colony Method (Illumina) and total DNA was quantified flourometrically. The extracted DNA was processed processed for library preparation with DNA Prep (M) Tagmentation kit (Illumina) using IDT for DNA/RNA UD Index sets A-D (Illumina). Indexed libraries with an average insert size of 500 bp were pooled equally and sequenced.