RNAseq of Montipora capitata:Initial colony 1036 Pulse High
Tissue samples were collected with a dermal curette and imediately frozen in liquid nitrogen. Total RNA was extracted with Trizol reagent with Dnase treatment, followed by phenol-chloroform pufication. Tag-seq libraries were prepared using published methods (Meyer et al. 2011). Final libraries were sequenced as single-end 100 bp reads across two lanes of an Illumina HiSeq4000.