Control_Females_8
The PCR was done using Roche High-Fidelity PCR System (Roche Life Science, Welwyn Garden City, UK). A total of 5ng DNA was amplified in a reaction volume of 10l. The primers for the amplification of the 16S region V3-V4 Forward were 27F and 515R primers (16S V3-V4 F: ACACTGACGACATGGTTCTACA, 16S V3-V4 R: TACGGTAGCAGAGACTTGGTCT).