gDNA-seq of Lepisma saccharina: adult male whole body
Genomic DNA sample was extracted from a single adult male using Qiagen MagAttract HMW DNA Mini Kit (Qiagen, MD). gDNA concentration was measured on a Qubit 3.0 Fluorometer (Thermo Fisher Scientific, MA). The quality and size distribution were accessed on Agilent TapeStation 4200 (Agilent Technologies, CA) using Agilent Genomic DNA ScreenTape Assay. The DNA integrity number (DIN) is 9.3, suggesting perfect DNA integrity. A 10x Genomic library was constructed using the Chromium Genome Reagent Kits v2 on the Chromium Controller (10x Genomics Inc., CA) with 1.2 ng input DNA. After quality control, the library was sequenced on an Illumina HiSeq X machine at the Genomic Services Lab at the HudsonAlpha Institute for Biotechnology.