Glycine max
Total RNA of sample was extracted through RNeasy Plant Mini Kit (Qiagen) protocol. SuperScript III First-Strand Synthesis System was used to synthesize cDNA libraries (Invitrogen). RT-PCR was used to test cDNA quality with the help of primers pair for housekeeping genes. Sequencing was performed at 50X coverage on Illumina HiSeq 2500 platform using PE150 run.