RNA-Seq of mus musculus: adult SNc dopaminergic neurons
The extracted RNA was quantified using a NanoDrop spectrophotometer (ThermoFisher) and the RNA integrity was measured using RNA Nano Chips and Agilent 2100 Bioanalyzer (Agilent). The cDNA libraries were generated from purified mRNA using TruSeq RNA Sample Preparation Kits (v2, Illumina) according to the manufacturers instructions. The samples were sequenced with Illumina HiSeq 2000 (BGI, Cambridge, MA)