ChIPseq-EP300_mES_TIR1_ARID1A_mAID_0h_auxin rep
Cells were lysed in MNase lysis buffer (10 mM Tris pH7.5, 10 mM NaCl, 3mM MgCl2, 0.5% NP40, 0.15 mM spermine, 0.5 mM spermidine, plus Roche MiniComplete).and digested in MNase digest buffer (10 mM Tris pH7.5, 60 mM NaCl, 1 mM CaCl2, 0.15 mM spermine, 0.5 mM spermidine, 0.5 mM DTT) using 36 units of S7 MNase (Roche) for 20 minutes at 37C. The digest was stopped by adding 50 mM EDTA and cells were spun down. Cell pellets were resuspended in ChIP buffer (SimpleChIP Enzymatic Chromatin IP Kit, CST). ChIP was performed following the manufacturers protocol.