GSNOtreated rhd6 sample 4
Roots were collected from 5dold seedlings of WS2, rhd6, and IAA and GSNOtreated rhd6 and immediately frozen in liquid nitrogen. Four biological replicates (c. 300 roots) for each treatment were used for RNA isolation. Total RNA was isolated using an InviTrap Spin Plant RNA Mini Kit (Stratec Molecular, Birkenfeld, Germany) according to the manufacturer's instructions. The total RNA concentration and quality were determined using a nanophotometer Pearl (Implen, Munich, Germany) and a bioanalyser 2100 (Agilent, Santa Clara, CA, USA). Libraries were prepared as described in the TruSeq RNA Sample Prep Protocol (Illumina, San Diego, CA, USA). Indexed DNA libraries were normalized, pooled, and sequenced in the pairedend mode in two lanes using an Illumina HiSeq SQ sequencer, following the standard sequencing protocol recommended by the manufacturer.