Wivenhoe + Benzene 3rd enrichment (E3)
Genomic DNA were extracted from  sediment slurry microcosms from Colne estuary, UK. The 16S V3 and V4 region was amplified using the forward primer  (5-CCTACGGGNGGCWGCAG -3)  and the reverse primer  (3- GACTACHVGGGTATCTAATCC -5)