POL3 Machinery-ChIP-Seq for HSV-1 Productive Infection in MRC5 cells
MRC5 cells were mock-infected, infected with ICP4 null mutant (n12) for 2 hours or infected with wildtype HSV-1 (KOS) for two, four or six hours at an MOI of 10 PFU/cell. ChIP-seq was performed for POLR3A, BRF1, GTF3C5, TBP, GTF3A, or BRF2. Matched input chromatin was also sequenced. Illumina sequencing was performed using HiSeq2500 to generate 50 SE reads, adaptors are Illumina TruSeq 6 base.