small RNA collection of symptomatic potato (Solanum tuberosum L.) plants of several cultivars
After grinding with mortar and pestle, RNA was extracted from 500 mg of ground material by TRIzol reagent (Invitrogen), following the manufacturer's protocol. After resuspension in RNase-free water, the RNA was re-precipitated in ethanol, stored at -80C, prior to NGS analysis.Total RNA was used to prepare a small RNA fraction by Tel Aviv University, and from the total RNA three libraries (SA483, 484 and 485) were prepared for Illumina sequencing (Technion Genome Center, Technion University, Haifa, Israel) which were used in a single Illumina HiSeq 2000 flow cell with three unrelated libraries