The sequence of messenger RNAs (mRNAs) originating from Lasiodiplodia theobromae strain CGMCC3.20151 (namely GZHS-2017-010) treated with thymol EC25 in PDB for 12 h
Mycelial plugs (6 mm diameter) from the edge of 3-day-old colony of the strain CGMCC3.20151 (namely GZHS-2017-010) was transferred to PDB and inoculated in shaker with 25C, 180 rpm for 36 h. Mycelia treated with thymol (EC25, finally concentration: 25.7546 g/mL) for 12 h were ground into fine powder under the protection of liquid nitrogen. One hundred mg mycelia powder were used for RNA extraction. Total RNA was isolated and purified using Trizol reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer's procedure. We performed the 2  150 bp paired-end sequencing on an Illumina Novaseq 6000 (LC Bio, China) following the vendor's recommended protocol.