DNA-seq of mchenga conophoros: adult diencephalon
Genomic DNA was isolated from diencephalic tissue s ampled from each test subject using a DNeasy Blood and Tissue Kit pipeline with a 60 min lysis time and without RNase A. The 260/280 nm absorbance ratio ranged from 1.91 - 2.10 across subjects. Libraries were prepared following a NEBNext Ultra II FS DNA Libr ary Prep Kit for Illumina protocol. Libraries were sequenced on two NovaSeq 6000 lanes using 300 - cycle SP Reagent Kits ( 2x150 bp paired - end reads; Illumina).