Ssedi2_L001_R1.fastq
DNA from a single colony was isolated using the E.Z.N.A. Tissue DNA Kit (Omega Biotek, Norcross, Georgia, USA), strictly following the manufacturers instructions. A negative control composed of only DNA extraction solutions was included in the DNA extraction procedure to check for cross-contamination. Genomic libraries were prepared using the Nextera XT Library Prep kit (Illumina, Massachusetts, Canton, USA), strictly following the manufacturers instructions. The distribution of fragment lengths was checked in the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, California, USA), using the Agilent High Sensitivity DNA Kit (Agilent Technologies). The fragments were then pooled in equimolar amounts according to the quantification provided by the Qubit ds-DNA HS Assay (Thermo Fisher Scientific, Waltham Massachusetts, USA). The pooled genomic libraries were sequenced using a MiSeq PE300 run (Illumina).