RNAseq of 15dpf Danio rerio (5 pooled whole larvae): Nrf2a WT DMSO
RNA was isolated using the RNA Clean Concentrate Kit with in-column DNase-I treatment (Zymo Research Corp.), following manufacturer instructions. The quantity of RNA was assayed on a Qubit using an RNA BR assay (Life Technologies Corp.), and the quality was assessed on an Agilent 2100 Bioanalyzer using an RNA 6000 Nano Assay (Agilent Technologies Inc). Total RNA was used to isolate poly(A) mRNA and libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturer instructions. The quantity of the library was assayed using a Qubit DNA HS assay (Life Technologies Corp.), and the quality was analyzed on a Bioanalyzer (Agilent Technologies Inc). Libraries were pooled and sequenced on an Illumina NextSeq 500 platform with 76 bp paired-end sequencing chemistry.