WGBS of Homo sapiens-1
Isolated genomic DNA was sheared into fragments of 100300 bp in length. The 3 and 5 overhangs were repaired to create blunt ends, and a single A was added to the 3 end of the blunt fragment. Methylated adapters were ligated to the A-tailed fragment and bisulfite modified using a ZYMO EZ DNA Methylation-Gold Kit. A final polymerase chain reaction (PCR) amplification step was then performed for library qualification. Only the qualified library with the targeted size range was sent for sequencing.