bulk RNA-seq of hiPSC-derived GABAgeric neurons: 40 days in vitro
Total RNA from neurons was extracted using a TRIzol reagent kit (Invitrogen, Carlsbad, CA, USA). RNA integrity was checked on an Agilent 2100 BioAnalyzer to conduct quality control (Agilent Technologies, Santa Clara, CA, USA). Library construction and high-throughput RNA sequencing (RNA-seq) were performed with the HiSeqTM 4000 sequencing platform (Illumina, California, USA).