CRISPR Screen
NNNNNNGGCTTTATATATCTTGTGGAAAGGACGAAACACCGNNNNNNNNNNNNNNNNNNNNGTTT in which the first 6 N's represents the sample barcode and the 20 N's the CRISPR guide. Barcodes: CGTGAT, ACATCG,GCCTAA: Pre-adhesion; TGGTCA,CACTGT,ATTGGC: anti-IgM; GATCTG, TCAAGT,CTGATC: PMA. CRISPR guides: Addgene #1000000082.