RNA-Seq of gut microbiome: human fecal
Purified PCR products from the fecal samples were combined at equal concentrations and used to construct a metagenomic library using an Illumina TruSeq sample preparation kit (Illumina, San Diego, CA, USA) according to the manufacturers protocol. Barcoded V3-V4 amplicons were sequenced using a pair-end method via the Illumina Miseq (Illumina, San Diego, CA, USA) sequencing platform with a 6-cycle index read.