Accurate profiling of forensic autosomal STRs using the Oxford Nanopore Technologies MinION device
Six full PCR reactions per sample were prepared with the Promega PowerSeq 46GY System (PS4600) according to the manufacturers technical manual with 0.5ng input DNA. Amplification was performed in triplicate on an Eppendorf Mastercycler pro S using the recommended thermal cycling conditions at either 15 or 30 cycles.