RNA-Seq Splicing Factor Analysis in Mus musculus for Retinitis Pigmentosa: Retina
Total RNA was extracted per the manufacturers instructions. RNA quality and quantity was assessed on an Agilent 2100 Bioanalyzer, RIN number ranged between 9.6-9.9. For each sample 1mg of total RNA spiked with 1.2ng Sequins (v2) controls was used to generate RNA-seq paired-end libraries with the Illumina TruSeq Stranded Total RNA kit. Ribosomal RNA was removed with the Ribo-Zero Human/Mouse/Rat kit. Libraries were multiplexed and sequenced on an Illumina HiSeq 2500 instrument for 101 cycles.