RNA-seq of synovial tissue: ustekinumab treatment
Total RNA was extracted from snap-frozen synovial biopsies using RNA Stat-60, treated with DNase I (Invitrogen), and cleaned using RNeasy columns (Qiagen). cDNA libraries were constructed with Illumina TruSeq RNA Sample Preparation Kit v2 (Illumina) using 1 g of total RNA with RNA integrity number (RIN) 7. The resulting cDNA libraries were paired-end sequenced on Illumina NovaSeq 6000 by Macrogen (Seoul, Korea).