genome resequencing of Bupleurum smithii
The total genomic DNA was extracted following the CTAB method (Doyle and Doyle 1987). After DNA extraction, 1g genomic DNA was randomly fragmented by Covaris, followed by fragment selection by Agencourt AMPure XP-Medium kit to an average size of 200400 bp. Then the genomic library (paired-end, PE 150 bp) was sequenced on an Illumina Hiseq 4000 platform at Beijing Genomics Institute (Shenzhen, China)