transcriptomics  analysis reveals the molecular mechanism of salt tolerance in Brassica napus during germination stage
The experimental materials included Huayouza 62 (H62) and Zhongshuang 11 (ZS11). Seed germination experiment method using previously described by Wan et al (2018)[31]. The seeds of H62 and ZS11, which are full and of the same size, were evenly sown in the corresponding area at the bottom of the culture dish. 50 seeds are sown for each material in each dish. 0 mM, 100 mM, 150 mM and 200 mM NaCl solutions were performed to salt stress. On the 8th day after sowing, 8-10 seedlings with relatively consistent growth were selected for each treatment, and the hypocotyl length and main root length were measured; 3 seedlings were selected for each treatment and mixed into a biological sample, each group had 7 biological repeats, which were stored in the refrigerator at - 80  by liquid nitrogen quick freezing for use (because the seedlings did not grow after the 200 mM NaCl solution of Zhongshuang11, and the two materials were at 100 mM salt treatment can promote seedling growth, so neither of these two processes were sampled). Finally, Huayouza 62 took three groups of samples, named H62-CK, H62-T1, H62-T2, Zhongshuang11 took two groups of samples, named ZS-CK and ZS-T1, in which CK was 0 mM, T1 was 150 mM, T2 was 200 mM. The samples were determined and analyzed by BGI.