Whole-exome sequencing of gout blood
Genomic DNA was isolated from peripheral blood according to standard procedures using a Qiamp Blood DNA mini Kit (Qiagen, Hilden, Germany). About 2 g of genomic DNA sample was randomly sheared to 200-250 base pairs (bps) in size using a Covaris S220 System, after which the fragments of DNA were ligated to attach Atails and adapters to both ends. After amplification, purication, and hybridization of adapterligated DNA, as well as the removal of nonhybridized fragments, capture and enrichment of exome regions was conducted using an Agilent SureSelect Human All Exon V6 kit (Agilent Technologies, Palo Alto, CA, USA) according to the manufacturer's protocol. High-throughput library sequencing was performed on an Illumina HiSeq2000 Analyzer (Illumina, San Diego, CA, USA).