RNA-Seq of ginseng callus cultured 9 years
Total RNAs were isolated from liquid nitrogen-frozen ginseng callus of three subculture durations (9-y-old) using Trizol (Invitrogen) according to the manufacturers instructions. Integrity of the extracted RNA was determined using an Agilent 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany). Transcriptome libraries were constructed and sequenced using the Hiseq 2000 (BGI, Shenzhen, China) with standard protocols. Two biological replicates were conducted for each sample and sequenced as parallel experiments.