HC2_Mono
This is bulk RNAseq data. RNA was extracted from the aqueous phase with the miRNeasy Micro Kit on a QIAcube Connect. After RiboGreen quantification and quality control on an Agilent BioAnalyzer, 1-2 ng of total RNA with RNA integrity numbers of 3 to 10 was subjected to amplification with the SMART-Seq v4 Ultra Low Input RNA Kit, and 12-16 cycles of amplification. We then used 5-10 ng of amplified cDNA to prepare libraries with the KAPA Hyper Prep Kit, with eight additional PCR cycles. Samples were barcoded and analyzed on Illumina HiSeq 4000 sequencer.