RNA-Seq of non stressed trinucleate pollen from brassica napus
BGISEQ-500 platform for PE100 strand-specific mRNA sequencing with an expected output of 30 million raw reads per sample. After sequencing, the raw reads were filtered. Data filtering included removing adaptor sequences, contamination, and low-quality reads from raw reads. The trinucleate pollen sample collected from plants grown at control conditions was used for RNA isolation.