RNA-Seq of mus musculusadult male freshly isolated satellite cells
Total RNA of freshly isolated satellite cells was extracted using MagMAX-96 Total RNA Isolation kit (Ambion; AM1830). Then, RNA samples were pretreated with DNase and processed following Illumina manufacturer's instructions. PolyA+ mRNA was isolated with magnetic beads with oligo (dT) following by fragmentation. The first-strand cDNA was synthesized using random hexamer primer. The second-strand cDNA was then synthesized. The products were purified and resolved with QIAquick PCR Purification Kit (Qiagen). Purified cDNA fragments were connected with sequencing adapters and gel electrophoresed to select suitable fragments for PCR amplification. Agilent 2100 Bioanalyzer were used for quality control.