RNA-Seq of mus musculus
Total spermatogenic cells were extracted from the seminiferous tubules of adult male mice, stained by Hoechst 33342 (Acros Organics), and then separated through FACS sorting. The isolated spermatogenic cells were further confirmed by their distinct nuclear morphology (DAPI staining of nuclei). Total RNAs were extracted from mouse spermatocytes using RNAiso Plus (Takara) RNA libraries were prepared for sequencing using standard Illumina protocols.