#1 T cells isolated from female recipient-B6D2F1 mouse treated with vehicle;
B6D2F1 recipients (H-2b/d) were lethally irradiated and transplanted with 1 x 106 host allo-Ag-specific 2C TCR transgenic T cells (2C, CD8.1+) and 1 x 106 wild type T cells (WT, CD45.1+) of B6 background (H-2b) plus T-cell-depleted bone marrow cells (TCD-BM) from WT B6 (CD45.2+) on day 0. In some experiments, recipients were p.o. injected with 25 mg/kg cyclosporin A (CSP) daily from day 0.Next, we performed scRNAseq of donor T cells harvested from CSP- or vehicle-treated recipients on day 14 after allo-SCT.