RNA-Seq of TA-treated
The C. albicans strain was cultured in YPD medium for 12 h, and TA was addedwith concentrations of 4 g/mL(TA-4), 8 g/mL(TA-8)respectively. After beingtreated with YPD for 12 h, the cells of TA-treated C. albicans were collected, and thetotal RNA was extracted by RNA extraction kit (Umagen, China). And the integrity ofRNA was detected by HPLC (Aglient 2100, USA). The two cDNA strands weresynthesized by cDNA synthesis kit (NEB, New England Biolabs). Then the poly Awas added by using NEB Next End prep enzyme and blunt TA ligase, NEBNextAdaptor to prepare the cDNA library. The quality of which was identified by DNA1000 assay kit (Agilent, USA). The cDNA library was sequenced on the Illuminasequencing platform (Illumina HiSeq 2000) using the single-end paired-end (PE)technology within a single run. PE reads with length of 90 bp were obtained using theIllumina GA Pipeline (version 1.6). All the transcriptomes of different samples wereassembled using a PE 100 sequencing strategy.