RNA-seq of Aspergillus fumigatus A1160: WT-3
Fresh A. fumigatus conidia were grown in liquid MM in a rotary shaker at 220 rpm at 37C for 48 h. For RNA-seq analysis, the samples/mycelium pellets were collected and quickly frozen in liquid nitrogen. After mRNA extraction, purification and library construction, sequencing was performed by next-generation sequencing (NGS) based on the Illumina sequencing platform. A fold change of >2 and a P value of < 0.05 were set as the threshold values for differentially expressed genes. The detailed procedures were performed by Shanghai OE Biotech. Co., Ltd. (China) as a commercial service. Each sample was analyzed using three biological repetitions.