CCP panel sequencing of homo sapiens: microadenoma replicate 1 from pancreas
DNA was extracted from four formalin-fixed, paraffin-embedded tumor tissues by using GeneRead DNA FFPE kit (Qiagen, Germany), and from whole peripheral blood by using standard phenol-chloroform extraction protocol. One tumor sample was insulinoma, three others were microadenomas.DNA sequencing (NGS) was performed by using Ion AmpliSeq targeted amplification technology with AmpliSeq Comprehensive Cancer Panel (Thermo Fisher Scientific, United States) containing cancer-specific primer panel with exon coverage of 409 oncogenes and tumor suppressor genes. The bioinformatic workflow for sequencing data analysis was based on Torrent Suite software (version 5.10.1).