16S rRNAseq of cyprinus carpio intestinal chymus: biological replicate 3
Nine-month-old fish were anaesthetized in 0.3 g/L Tricaine Methane Sulfonate (TMS) (Crescent Research Chemicals, Phoenix, USA) in aquarium water buffered with 0.6 g/L sodium bicarbonate and bled via the caudal vein. The third segment of the intestine was isolated and intestinal content of the third segment was collected by scraping, snap-frozen in liquid nitrogen and stored at -80C. Total RNA from intestinal content was isolated using the RNeasy PowerMicrobiome Kit (Qiagen, 26000-50) and used for 16S amplicon rRNA sequencing