mtDNA-seq of Oenothera biennis strain suaveolens Grado: mature rosette leaves
Creation of shotgun libraries was done by using the NEBNext DNA Sample Prep Master Mix Set 1 kit. In brief, genomic DNA was fragmented using a Covaris E210 Instrument . Then end-repair, A-tailing and ligation of indexed Illumina Adapter, agarose gel size selection and amplification was performed. The resulting fragments were cleaned up, pooled and sequenced on a HiSeq 2000 at Eurofins MWG Operon with 2x 101 bp paired end reads.