RNA-Seq Splicing Factor Analysis in Mus musculus for Retinitis Pigmentosa: Brain
Total RNA was extracted per the manufacturers instructions. RNA quality and quantity was assessed on an Agilent 2100 Bioanalyzer, RIN number >7 for all the samples. For each retina, brain, muscle and kidney sample, 1g of total RNA was spiked with 1.2ng sequins (v2) controls. For RPE samples 100ng total RNA was spiked with 0.12ng sequin controlswas used to generate RNA-seq paired-end libraries with the Illumina TruSeq Stranded Total RNA kit. Ribosomal RNA was removed with the Ribo-Zero Human/Mouse/Rat kit. Libraries were multiplexed and sequenced on an Illumina HiSeq 2500 instrument for 101 cycles.