16S rRNA gene amplicons of offshore surface Lake Michigan microbial community from control mesocosms after 2 hours incubation: replicate 3
Nearshore surface, offshore surface and offshore deep (hypolimnetic) Lake Michigan water was collected in September 2015 and used to set up ~3 L triplicate mesocosms that either received 120 micromolar terrestrially-derived dissolved organic matter (tDOM) or were left unamended (control). The mesocosms were incubated in the dark and subsampled for ~1 L water at 2 h and 19 h to collect free-living prokaryotic cells. These cells were then used for whole-community DNA and RNA extraction using phenol-chloroform based organic extraction in the lab. For this library, community genomic DNA from one of the triplicate control mesocosms (2 h time-point) set up using offshore surface lake-water was used for 16S rRNA gene amplicon sequencing using the V3 to V4 primers 341F-806R.