16S rDNA of bacteria: strawberry soil
The primers are 338F (5-ACTCCTACGGGAGGCAGCAG-3) and 806R (5-GGACTACHVGGGTWTCTAAT-3). PCR amplification program was as follows: an initial activation step with 95C for 3 min, followed by 25 cycles at 95C for 30 s, 55C for 30 s, and 72C for 30 s and a final extension at 72C for 36 min. The amplified PCR fragments were purified with Agencourt AMPure XP beads to generate a sequencing library