16S rRNA gene amplicon of human feces
The V3-V4 regions of 16S rRNA gene was amplified using the following primer pair (forward 5'-TCGTCGGCAGCGTC-AGATGTGTATAAGAGACAG-CCTACGGGNGGCWGCAG-3' and reverse 5TCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC3)