RNA-Seq of Prickly wild type of Solanum viarum Dunal
The epidermis of prickly wild type from 45 days old in vitro grown plantlets was pealed out in liquid nitrogen. Epidermis from five plants were pooled and total RNA was isolated using Spectrum Plant Total RNA Kit and treated with the RNase free DNaseI to remove DNA contamination. The quality and quantity of total RNA were analyzed by agarose gel, spectrophotometric analysis and Agilent 2100 Bioanalyzer RNA chip.