week_17g_wheat rhizosphere  metagenome_replicate 1g
Shotgun metagenome libraries were prepared using the Celero DNA-Seq library preparation kit (NuGen, Takara Bio, USA) with enzymatic shearing, according to the manufacturers instructions. All libraries were then pooled in equal volumes and size selection (350400 bp fragments) was performed using a Blue PippinPrep instrument (Sage Scientific). The libraries were then sequenced using an Illumina MiniSeq instrument employing a mid-output kit. Based on the number of reads per sample, the samples were repooled with varying volumes, and size selection was performed again using the same size range. The final size-selected pool was sequenced on an Illumina NovaSeq instrument with an S4 flow cell, employing 2 x 150 base reads. Library preparation and pooling were performed at the University of Illinois at Chicago Sequencing Core (UICSQC), and sequencing was performed by Novogene Corporation (Chula Vista, CA, USA).