RNA-Seq of Rheum ribes 4-years-old roots
Using the IlluminaTruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA, USA), parallel sequencing libraries were constructed. Ultimately the transcriptome library per sample was sequenced by Illumina NovaSeq 6000 (Novogene-Advancing Genomics Improving Life, Beijing, China) and 150 bp paired-end reads were generated.