16s rRNA sequencing of DSS mice at day 10: feces
We established DSS-induced mice colitis model.DNA extraction and amplification Genomic DNA were extracted from fecal material using the E.Z.N.A. Stool DNAKit. PCR amplification were performed using 515F/806R Illumina primers targeting the V4 region of the 16S rRNA gene. Amplicons were sequenced using Illumina NovaSeq 6000 platform