Mitochondrial genome DNA-seq of Homo sapiens: HepG2, replicate 3
mtDNA was extracted from isolated mitochondria for sequencing. To reduce nuclear DNA contamination, the circular mtDNA was further enriched and purified by linear nuclear DNA digestion using an ATP-dependent exonuclease as reported by Gould et al. (PMID: 26488301) The concentration of samples was determined by Qubit fluorometer (Thermo) before sequencing. Sample quality control, DNA library preparation (KAPA low-input DNA library kit) and NGS sequencing (by HiSeq X Ten sequencing platform, 150 bp pair-end sequencing) was performed by Groken Bioscience LTD.