Bulk fecal bacterial sample from healthy donor: H37
Fecal DNA was isolated using the Quick-DNA Fecal/Soil Microbe Kit (Zymo Research) according to manufactures instructions. 16S rRNA library preparation was performed according to Illuminas 16S Metagenomic Sequencing Library Preparation protocol using the 16S V3 forward (341F) and V4 reverse (805R) primer pair with Illumina adapter overhang nucleotide sequences added. Sequencing was performed using the Miseq Reagent Kit V3 on the Illumina Miseq platform.