RNA-seq of Sp9512: turions
First strand cDNA synthesis was performed using random hexamer primers followed by second-strand cDNA synthesis. Library fragments of 150-200 bp were purified with the AMPure XP system and amplified with Phusion High-Fidelity DNA polymerase. Pair-end sequencing (2 x 150 bp) was performed on libraries using the Illumina Novaseq6000 S4 sequencing platform.