human urinary tract microbiota, Chlamydia trachomatis infection, Sample: U_137
Each enrolled woman provided the first void of the first urines of the morning. 2.5 mL were used for CT, Neisseria gonorrhoeae, Trichomonas vaginalis and Mycoplasma genitalium detection by commercial nucleic acid amplification techniques (NAATs) (Versant CT/GC DNA 1.0 Assay; Siemens Healthineers, Terrytown, NY, USA; Aptima Trichomonas vaginalis and Aptima Mycoplasma genitalium assay, Panther system, Hologic, Marlborough, MA, USA, respectively). Starting from the remaining eluate of Versant PCR plate, hypervariable V3-V4 regions of the bacterial 16S rRNA gene were amplified, according to 16S metagenomic sequencing library preparation protocol (Illumina, San Diego, CA). Final indexed libraries were prepared by equimolar (4 nmol/L) pooling, denaturation and dilution to 6 pmol/L before loading onto the MiSeq flow cell. A 2  300 bp paired-end run was used.