DNA-seq of Drosophila mauritiana: whole adult males
We extracted DNA from 20 whole male flies of each line using a modified phenol:chloroform protocol. We prepared libraries using the Ligation Sequencing Kit 1D with native barcoding (SQK-LSK109 and EXP-NBD104, Oxford Nanopore) according to the manufacturers protocol. Libraries were sequenced on six R9.4 flow cells with an average of 1,526 available pores and run for 48 hours, or until no pores were available, on a GridION running MinKNOW v3.1.8.