RangerPro_High_Females_7
The PCR was done using Roche High-Fidelity PCR System (Roche Life Science, Welwyn Garden City, UK). A total of 5ng DNA was amplified in a reaction volume of 10l. The primers for the ITS2 amplification were (ITS3-F: GCATCGATGAAGAACGCAGC, ITS4-R: TCCTCCGCTTATTGATATGC).