RAD-seq of Podarcis hispanicus complex' contact zones and reference populations.
Digestion of genomic DNA with SbfI and MseI enzymes; ligation of barcoded SbfI adapter and MseI adapter; amplification using P5 and P7 (indexed) Illumina primers (single index) in four independent PCR reactions; PCR products pooling and fragment selection between 400 and 500 bp using a 2.5% agarose gel.