RNA-Seq of Lippia alba: linalool accession leaves
The unexpanded leaves at the fourth nodal segment were collected and macerated in liquid nitrogen using sterile RNAse-free. Total RNA was extracted using the PureLinkRNA Mini Kit (Ambio) from Life-Technologies, following manufacturer's instructions. The RNA analysis, libraries construction and Next Generation Sequencing were performed by the High-Performance Center Genome Sequencing - Genomics-DF, at the Catholic University of Braslia - Federal District, Brazil. The total RNA sample was analyzed quantitatively and qualitatively, using a Bioanalyzer equipment Agilent 2100 System. The preparation of the libraries was carried out using the TruSeq Sample Preparation Kit (Illumina), following the recommendations of the manufacturer; . A run of 2x300 bp, or 600 cycles, with paired-end libraries was performed at the MiSeq of Illumina sequencing platform.