Response of Endozoicomonas 6c to host tissue extract
Total RNA was extracted using the RNeasy Mini Kit (Qiagen, Germany). To remove genomic DNA, a DNAse treatment was performed. Ribosomal RNAs and eukaryotic mRNAs were depleted using the AmbionTMMICROBEnrich Kit and the MEGAclear TranscriptionClean-Up Kit. RNA-Seq of Endozoicomonas 6c culture (isolated from central Red Sea coral Acropora humilis) exposed to: a) control conditions (28C); b) coral host tissue extract; c) heat stress (33C).