RNA-seq of Arabidopsis: leaf
Total RNA was extracted from WT and cdb1 leaves using Trizol reagent kit (Invitrogen, Carlsbad, CA, USA), and rRNAs were removed by Ribo-ZeroTM Magnetic Kit (Epicentre, Madison, WI, USA) to retain mRNAs and other RNAs. Enriched RNAs were fragmented and reverse transcribed into cDNA with random primers. Second-strand cDNA was synthesized, end repaired, and ligated to Illumina sequencing adapters using NEB#7490 kit (NEB E7490L, New England Biolabs). The ligation products of 300-500 bp were selected by agarose gel electrophoresis, PCR amplified and sequenced using Illumina HiSeq2501 (Gene Denovo Biotechnology Co).