Salmonella assessment on antibiotics resistance gene
Salmonella was isolated from goose carcasses purchased at open markets, following United State Department of Agriculture procedure (MLG4.10). Genomic DNA cleavage and adapter attachment for sequencing reaction Purification of products after genomic DNA cleavage reaction using QIAquick kit. The QIAquick PCR purification kit (Qiagen) is used to purify the product of genomic DNA cleavage by silica absorption column. Setting up the PCR reaction with the indicator (Index) using the Nextera . kit Purification of PCR products Measure DNA concentration by KAPA kit (nM), dilute/normalize and pool genomic DNA samples