RNA-Seq of Schedorhinotermes_sp4:head from worker, isolate 2
Termites were collected in RNA-later. Each sample was temporarily stored in the field at temperature varying between -20C and 4C, and then kept at -80C until RNA extraction. We dissected with a scalpel the heads of 2 to 15 individuals, predominantly workers, and transferred them in 400 l of TRIzol Reagent (Invitrogen). Heads were homogenized using a Fisherbrand Disposable Pestle System (Fisher Scientific). We extracted total RNA using a standard phenol-chloroform procedure with TRIzol according to manufacturers protocol. Extracted RNA samples were treated with TURBO DNase (Ambion) at 37C for 1h, and purified using the RNA Clean & Concentrator-5 kit (Zymo Research). RNA samples were poly(A)+ enriched and fragmented.