mtDNA-seq of Oenothera elata ssp. hookeri strain johansen Standard: mature rosette leaves
100 ng mtDNA were initially fragmented by sonication, followed by library preparation with NEBNext Ultra Directional DNA Library Prep Kit for Illumina includinged 9 cycles of PCR amplification. Libraries were immobilized and processed onto a flow cell with cBot and subsequently sequenced on a HiSeq 3000 system with 2x 150 bp paired end reads.