Norilo-Pyasino oil spill
The V3-V4 region of the 16S rRNA gene was amplified using primers 343F and 806R containing adapter sequences (Illumina), linker and barcode. Amplicons were mixed at 200 ng each and purified using the MinElute Gel Extraction Kit (Qiagen, USA). Sequencing was performed at the Genomics Center for Collective Use of the SB RAS (ICBFM SB RAS) on a MiSeq sequencer (Illumina, USA) using the Reagent Kit v3 (2x300, Illumina).