RNA-seq of 15 different pepper cultivars
The 100 mg of ground powders were subjected to total RNA extraction using RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) according to manufacturers instruction. The quality of extracted total RNAs was measured by the Technologies 2100 Bioanalyzer (Ag-ilent, Santa Clara, California, U.S.A.). Total RNAs with an RNA Integrity Number (RIN) value greater than or equal to 7 were further used for library preparation. Libraries for RNA sequencing were prepared using TruSeq Stranded Total RNA with Ribo-Zero Plant kit (Illumina, San Diego, California, U.S.A.) by depleting ribosomal RNAs from the total RNAs according to manufacturers instruction.