Human leukocyte antigen (HLA) region sequencing from a pan cancer patient cohort.: Sample WXS_N_DASH_20
Hybridization-based enrichment for exome preparation was carried out using target-specific biotinylated probes binding the desired fragments of the DNA library in solution.  The fragment-probe duplexes were then isolated using streptavidin-coated beads via magnetic pull-down, amplified by PCR, and purified with SPRI beads. Target-enriched libraries were quantified by qPCR and sequenced on Illumina instrumentation following the manufacturer's protocols. All reads that map to the HLA region using 30 base pair seeds were aggregated.