Amplicon sequencing of Drosophila suzukii: adult whole animal
PCR primers were designed to conserved target regions of the Drosophila suzukii genome. PCR was performed on genomic DNA pooled from many individuals to generate amplicons <450 bp long. Library preparation was performed using the NEBNext Ultra DNA Library Prep kit. Adapters were ligated and enriched by limited cycle PCR. Sequencing was performed using 2x 250 paired end configuration.