GSM6723963: AXGX4days; Mus musculus; RNA-Seq
The anterior lobe of freshly dissected pituitaries was minced and incubated for 15mn at 37ºC in 1mg/ml Papain (Sigma 10108014001), 10mg/ml DNAse I (Sigma 10104159001) and 10mg/ml Rock inhibitor (Abmole Bioscience M1817) in HBSS (Sigma H9394). pH was adjusted by addition of NaOH. The enzymatic solution was removed, and the cells were mechanically resuspended in HBSS supplemented with DNAse I and Rock inhibitor, as above. Cells were FACsorted for GFP using a large nozzle (100mm) and low pressure (20psi) for optimal cell survival. Library was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics).