GSM5391649: shints11_3h_r2; Homo sapiens; ncRNA-Seq
Protected from light. Total RNA was extracted using Trizol reagent (Thermo Fisher Scientific, #15596026) according to the manufacturer's instructions. Indicated samples were treated with Iodoacetamide (+IAA) for thiol modification. Genomic DNA was removed by Turbo DNAse treatment (Invitrogen, #AM1907). Small RNA libraries were prepared using the SMARTer smRNA-seq kit (Takara, #635030) with 800 ng of total RNA; 5% (40 ng) of Drosophila RNA were added as spike-in