GSM6892019: RNA-seq_Blastocyst_SCNT_rep2; Macaca fascicularis x Macaca mulatta; RNA-Seq
ICSI and SCNT blastocyst were lysed in lysis buffer containing RNase inhibitor, and the cDNA was synthesed using SMART-Seq v4 Ultra Low Input RNA Kit for sequencing (Clontech, 634888) according to the user manual. After amplification, the sequencing libraries were made with the fragmented cDNA using TruePrep DNA Library Prep Kit V2 for Illumina according to manufacturer's instructions (Vazyme, TD503). Libraries were quantified using a Qubit dsDNA HS Assay Kit (Thermo Scientific, Q32854), quality-controlled using a Fragment Analyzer instrument (Agilent), and paired-end 150 bp sequencing was performed on a Novaseq 6000 sequencer (Illumina).