GSM6617792: S1423, VS2, snRNAseq; Homo sapiens; RNA-Seq
OCT-embedded hemangioblastoma samples were removed from -80°C storage and placed in a cryostat. A clean razor blade was used to cut away OCT fragments. Nuclei were isolated from the frozen tissues using an adaptation of a previously established protocol. Samples was placed on a clean petri dish and washed with 800uL of Detergent-Lysis Buffer (0.1% Triton-X). Tissues were chopped into small pieces, homogenized in a 7mL Dounce Homogenizer on ice (Fisher Scientific # 501945204), and passed through a 70 m MACS strainer (Miltenyi Biotec # 130-098-462). Library was performed according to the manufacter's instructions (single nucleus protocol, 10x Genomics).