GSM5509551: Grafted Stromal cells SI; Mus musculus; RNA-Seq
Enlarged grafts were dissected out and made into single cell suspensions and sorted (Live, CD45-, Ter119-, BP3-, CD31-, EpCAM-, L1CAM-, Itgb1+, YFP+) MSCs sorted for scRNA-seq. Single cell RNA-seqg libraries were prepared according to the manufacturer's instructions using Chromium Single Cell 3' Library & Gel Bead Kit v3 (10x Genomics, PN-1000092) or 5' kit Chromium Single Cell 5′ Library & Gel Bead Kit (10x Genomics, PN-1000006), and Chromium Chip B Single Cell Kit (PN-1000074) with the Chromium Controller & Next GEM Accessory Kit (10x Genomics, PN-120223).