GSM6765131: AM7_01_H3K9me3_CHIP_Control; Homo sapiens; RNA-Seq
Chromatin immunoprecipitation was performed using the SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) (Cell Signalling Technology) following the instructions of the manufacturer. 10 μg of chromatin was incubated overnight with 1.6μg of the sheep anti-TIAM1, rabbit anti-TRIM28 or rabbit anti-H3K9me3 antibody. ChIP-seq libraries were prepared using 1ng of ChIP DNA and 12 cycles of amplification with the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs, Cat No:  E7645L). Libraries were quantified by qPCR using a KAPA Library Quantification Kit for Illumina sequencing platforms (Roche, Cat No: 07960336001). Paired-end 75bp sequencing was carried out by clustering 1.7pM of the pooled libraries on a NextSeq 500 sequencer (Illumina Inc.).