GSM6235097: SN_0_7; Ustilago maydis; RNA-Seq
Total RNA was extracted using GeneJET RNA Purification kit (ThermoScientific, Lithuania) according to the manufacturer recommendation for yeast total RNA isolation. DNA was removed from total RNA sampels using DNAfree DNase treatment and Removal Kit (Ambion). Purified RNA was analzyed by RNA 6000 Nano assay on Agilent 2100 Bioanalyzer (Agilent Technologies, CA, USA). Samples were further processed by Novogene as follows: messenger RNA (mRNA) was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dTTP. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries were pooled and sequenced on Illumina NovaSeq 6000 platform using 150bp paired-end sequencing.