GSM7039719: Zea mays Root Nuclei_2; Zea mays; RNA-Seq
Root tip were incubated in a enzymatic solution to digest cell walls and produce protoplasts, or in lysis buffer for nuclei extraction. Protoplasts or nuclei were filtered using a cell strainer and used as input for the 10x Chromium Single Cell 3' kit Libraries were prepared using Chromium Single Cell 3' Reagent Kit V2 and V3 protocol