GSM6543578: ATF2_OE, Input; Homo sapiens; ChIP-Seq
The cell was fixed in 1% formaldehyde for 10 min at room temperature. after which 0.125 M glycine was added and the mixture was sat for 5 min to terminate the crosslinking reaction. The grinded cells was treated with cell lysis buffer and nucleus was collected by centrifuging at 2000g for 5min.Then, nucleus was treated with nucleus lysis buffer and sonicated to fragment chromatin DNA. ChIP-seq libraries were prepared using VAHTS Universal DNA Library Prep Kit for Illumina V3（Catalog NO. ND607, Vazyme）. The library products corresponding to 200-500 bps were enriched, quantified and finally sequenced on Novaseq 6000 sequencer (Illumina) with PE150 model.