GSM7006871: cortex,rep2; Mus musculus; OTHER
Tissue was homogenized in Trizol and total RNA was isolated using the Zymo RNA Clean & Concentrator-5 (Zymo R1014) kit. For detailed protocol, see Nat Prot (link). First strand cDNA synthesis was performed by reverse transcribing polyA mRNAs using an oligo(dT) primer that also adds on a common primer sequence. RNA/DNA duplexes were removed by RNase H treatment. TdTomato self-reporting transcripts (SRTs) were amplified by PCR. PCR products were tagmented and indexed using the Illumina Nextera XT library preparation kit. For detailed protocol, see Nat Prot (link).