GSM6588412: DM_OP2_NM_6d_2 mRNA; Mus musculus; RNA-Seq
After two weeks, the transplanted mice were euthanised and bone marrow cells were harvested and stained with BV510 anti Lineage (CD3, CD19, Ter119, CD11b, Gr1) and APC anti c-Kit antibodies. For single-cell RNAseq we FACS-sorted Lineage- and Lineage+/cKit+ fractions and processed each of them in a 10X single-cell RNA-seq partition aimed at a final coverage of 500 single-cells per sgRNA. Single-cell libraries were generated using the Chromium Next GEM Single-Cell 3' Reagent Kits v3.1 (Dual Index) using the 10x Genomics manufacturer recommended protocol and targeting 16,000 cells per library.The resulting libraries were quantified using Qbit and qPCR and analysed on a Tape Station. Libraries were sequenced in a NextSeq 1000 or a Novaseq 6000 to a final coverage of 50,000 reads/cell for 3' Gene Expression libraries and 5,000 reads/cell for CRISPR Feature Barcode libraries.