GSM5509515: D4_K20R_rep2; Mus musculus; ATAC-seq
Cells were resuspended in 50 μL lysis buffers (10 mM Tris-HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl2, 0.2% (v/v) IGEPAL CA-630). Then the suspension of nuclei was centrifuged 500 × g at 4°C for 10 min. The pellet was resuspended with 50 μL transposition reaction mix (10 μL TD buffer, 5 μL Tn5 transposase and 35 μL nuclease-free H2O), and incubated at 37°C for 30 min. Finally, DNA was isolated using MinElute PCR Purification Kit (QIAGEN). ATAC libraries were prepared for sequencing using standard Illumina protocols.