GSM6617518: DRN-BA(Pyr) Neurons [PN2]; Mus musculus; RNA-Seq
With visual control under a Nikon Eclipse FN1 microscope (Japan), dsRed-positive neurons were slowly aspirated into a micropipette with a 70–100-μm tip diameter. The harvested neurons are immediately transferred into a clean centrifuge tube containing 2 μl of RNAse-free lysis buffer, with total mRNA subsequently isolated. Complementary DNA (cDNA) and indexed cDNA libraries were prepared using a SMART-Seq Stranded Kit (Cat. Nos. 634442, Takara Bio USA, Inc.), and the experiments were performed according to the manufacturer's standard protocols.