GSM5509260: Exponential-to-stationary phase transition (6h), replicate 3; Escherichia coli; RNA-Seq
Total RNA was extracted using ExtractRNA reagent (Evrogen) according to the manufacturer's protocol. RNA sample was treated with DNAse I (ThermoFisher Scientific) and purified with RNAClean XP beads (Beckman Coulter). Sequencing libraries were prepared without rRNA depletion using NEBNext Ultra II Directional RNA Library kit (NEB) with the following modifications: 10 minutes of fragmentation instead of 15 and 10 PCR cycles. Then they were quantified using qPCR, pooled in equal amounts, diluted to 2 nM and sequenced on HiSeq4000 instrument (Illumina, USA) with 50 bp single-end reads protocol. Libraries preparation and sequencing were performed at Skoltech Sequencing Core Facilities. RNA-Seq was performed in triplicate.