GSM6235074: Liver NK, control, rep3; Mus musculus; RNA-Seq
200 ILC1s [CD3(-)NK1.1(+)NKp46(+)CD49a(+)CD49b(lo)IL-7R(+)] and NK cells [CD3(-)NK1.1(+)NKp46(+)CD49a(-)CD49b(+)] were sorted from the liver and the spleen by BD FACSAria 3, and cells were lysed by Buffer RLT (QIAGEN). Then total RNA was harvested using Agencourt AMPure XP (Beckman Coulter) size selection beads. Takara SMART-seq HT kit (Takara Bio) was used with all extracted RNA for cDNA synthesis. Takara SMART-seq Library Prep kit (Takara Bio) was used with 1 ng of cDNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Takara HT Plus kit protocols