GSM6311286: AB/Tub larvae, 40.5 uM Ralox, Rep 5; Danio rerio; RNA-Seq
Total RNA was extracted from each sample of 20 larvae using the Norgen BioTek Corp Total RNA Purification kit (Norgen Cat#17200, Thorold, ON, Canada) with the optional RNase-Free DNase I kit (Norgen Cat#25710, Thorold, ON, Canada). Sequencing libraries were prepared using the Illumina Truseq Stranded mRNA kit using 1ug of total RNA.