GSM5970984: scRNAseq_islets_ln_TCRseq; Mus musculus; RNA-Seq
CD8+ T cells were FACS sorted from lymph node and islet preparations. Islets were isolated by injecting collagenese solution in the clamped pandcreatic duct. Pancreata were then excised and incubated in collagenase for 30 mins at 37 degrees C and then washed and spun down twice ti distrupt the pancreas. Islets where then picled under a dissecting microscope and incubated for 15 mins at 37 degrees C in dissociation buffer (Life Technologies). CD8+ T cells were then sorted from these single cell suspensions. For bulk RNAseq, libraries were generated using Smart-seq2. For scRNAseq, 10X Genomics 5' v1 kit was used in conjunction with Total-SeqC antibodies as per the manufacturer's instructions. Libraries were generated from scRNAseq, scATACseq, and CITEseq. For scATACseq, 10X Genomics Multiome ATAC + Gene Expression kit was used as per the manufactuerer's instructions.