GSM5971473: Cardiac Endothelial Cells_md38phIC1_male_rep 4; Mus musculus; RNA-Seq
E12.5 hearts were lysed with Collagenase (Sigma-Aldrich), Dispase II (Sigma-Aldrich), and DNase I (Sigma-Aldrich). Cardiac endothelial cells were collected using MACS CD31 microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) and RNA was isolated using RNeasy Micro kit (Qiagen). After confirming RNA integrity using Bioanalyzer (Agilent Technologies, Santa Clara, CA), mRNA library was generated from 25ng RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module and Ultra II RNA Library Prep Kit (New England Biolabs). Library quality was assessed by Bioanalyzer (Agilent Technologies) and TapeStation (Agilent Technologies).