GSM6543711: cultured PP_CD11c+MHCII+SIRPa+ cells_scRNAseq; Mus musculus; RNA-Seq
PPs were harvested, incubated for 20 min at 37C in HBSS buffer with 5 mM EDTA and 5% fetal calf serum, and then digested for 45 min in RPMI media with collagenase D (Roche)/DNase I (Sigma) and 10% fetal bovine serum . Library was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics).