GSM5931596: Blastocyst derived extracellular vesicles replicate 3; Bos taurus; RNA-Seq
Firstly, Evs were isolated by qEV single size exclusion column (IZON Science, Cat. Number: qEV single ser. Y1000588) from blastocyst and non-blastocyst groups. Further on, 600 µL (× 6 replicates (3 blastocyst and 3 non-blastocyst) of the concentrated EV pellet from each group was used for total RNA extraction, using the Plasma/Serum Circulating & Exosomal RNA Purification Mini Kit (Norgen Biotek, Cat. 51000) according to the manufacturer's protocol. The quality and concentration of the RNA samples were examined using an RNA 6000 Pico Chip (Agilent Technologies) and a Quant-iT RiboGreen RNA Assay kit (Life Technologies), respectively. Small RNA library preparation was performed with the Tailormix v2 kit