GSM5509279: H2Aub-WT-MII-2; Mus musculus; ChIP-Seq
ChIP-seq was performed following the previously reported carrier DNA assisted ChIP-seq (CATCH-Seq) protocol. For each immunoprecipitation assay, 200-500 oocytes, zygotes or 2-cell embryos, as well as one microgram of H2AK119ub1 antibody (8240, Cell Signaling Technology) was used. The sequence libraries were generated using the NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645, New England Biolabs) following the manufacturer's instructions.