GSM5456206: G-line HOM rep3; Mus musculus; RNA-Seq
Total RNA was extracted from mouse left ventricles at the age of 2 month using TRIzol Reagent (Life technologies, Thermo Fisher Scientific, Waltham, MA) according to the manufacturer's protocol. DNase I (#D9905K, Lucigen, Middleton, WI) treatment was applied to clean up genomic DNA before RNA-seq. The quantity and quality were determined by Nanodrop One (Thermo Fisher Scientific, Waltham, MA) and electrophoresis. RNA libraries (Illumina TruSeq standard Total RNA Gold) were prepared for sequencing using standard Illumina protocols.