GSM5391522: Platelet Sample from Subject 182486, 2 Days Post Vaccination; Homo sapiens; RNA-Seq
Whole blood was collected in a yellow-cap tube and stored at room temperature. The tube was centrifuged and the platelet-rich plasma (PRP) was collected. The PRP was aliquoted and further centrifuged to isolate the platelet pellet. The platelet pellet was dissolved in 700 microliter Qiasol/TRISol. RNA was extracted using the Qiagen miRNA Easy Kit (Cat. # 217004) following the manufacturer's instructions using a QIACUBE. RNA-seq libraries were prepared using the Takara Bio SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian per the manufacturer's instructions.