GSM5509949: m-z+Dppa2_1C_totalRNA_1; Mus musculus; RNA-Seq
Embryos were lysised and the lysates were used as input for RNA-seq library constructions. The total RNA-seq libraries were prepared using the SMARTer-Seq Stranded kit (Takara). For polyA RNA-seq, cDNA was synthesized using the SMARTer Ultra low Input RNA cDNA preparation kit (Takara) and libraries were constructed using the Nextera XT DNA Library Preparation Kit (Illumina).