GSM5510068: EC_Week12_CBFGLIS_CordBlood_rep4; Homo sapiens; RNA-Seq
Total RNA from cultured cells were extracted with AllPrep DNA/RNA/miRNA Universal Kit using the QIAcube platform at 1 week, 6 weeks, and 12 weeks in culture. Total RNA (500ng) from cultured cells were prepared using the NEBNext rRNA Depletion Kit for Human/Mouse/Rat  (NEB, E6310X). The rRNA depleted RNA samples underwent the NEBNext Ultra Directional Second Strand cDNA Synthesis protocol (NEB) that incorporates dUTP in the dNTP mix.  The 75bp strand-specific paired-end mRNA libraries were prepared using the ribodepletion (rRNA depleted) v2.0 protocol by the British Columbia Genome Sciences Center (Vancouver, BC) and sequenced on the Illumina HiSeq 2500.