GSM6808274: KO_24h_rep1; Mus musculus; RNA-Seq
Chromatin-associated (nascent) RNA was extracted in TRIZol reagent. RNA was converted into strand-specific paired-end sequencing libraries using th TruSeq stranded total RNA kit (Illumina).  500ng of total RNA was rRNA-depeleted.  Chromatin-associated RNA containing less than %5 of rRNA based on bioanalyzer analysis was not rRNA depleted.  50ng of chromatin associated or rRNA-depleted total RNA was fragmented, converted to cDNA, adapter-ligated with unique indices and PCR amplified.  The final libraries were quantified and sequenced (NextSeq 500, Illumina) using paired-end 75bp chemistry.