GSM5390912: SEQ53; Homo sapiens; RNA-Seq
RNA was extracted using Universal (DNA/RNA/microRNA) kit from Qiagen The RNAseq libraries were generated using the TruSeq Stranded mRNA kit protocol. In brief, first the integrity of the RNA was checked with Agilent Tapestation, samples with RIN >7 were used for library preparation. The mRNA was purified from 1ug of total RNA for each sample, after cDNA synthesis and adapter ligation, the library was amplified with 12 rounds of PCR cycles.  The enriched and cleaned libraries were pooled and sequenced on the NextSeq500 using a 75 cycle high sequencing kit.