GSM5388380: N7_AM5; Oryza sativa; RNA-Seq
Total RNA was extracted from the tissue using Plant Total RNA Mini Kit (Favogen) Library construction was conducted according to the CEL-Seq2 method (10) with modifications. Briefly, 100 pg of RNA were used for amplification by in vitro transcription. Libraries were amplified by 11 -15 cycles of PCR, which was empirically determined.