GSM5819123: Hela_consi1; Homo sapiens; RNA-Seq
Cells were collected 48 hours post-transfection for RNA extraction. Total RNA was extracted using Trizol. Cytoplasmic and Nuclear RNA was extracted using RLN1 and RLN2 buffers lysing cytoplamic and Nuclear RNAs sepcifically followed by Trizol extraction. RNA libraries were prepared for sequencing using TruSeq Stranded Total RNA with Ribo-Zero from Illumina (Cat # RS-122-2201). mRNA sequencing on 100PE of DNBseq platform