GSM7047842: 19gtGPRC5AKOtrTNFArp1-6KOTNFA; Homo sapiens; RNA-Seq
Cells were washed with cold DPBS, and RNA were purified using RNeasy Plus Kit (Qiagen). 400 ng total RNA from each sample was used to prepare indexed sequencing libraries using the New England Biolabs Ultra II RNAseq kit for Illumina with the polyA module and 10 cycles of PCR. Libraries were size selected with Ampure beads to approximately 300 base pairs, quantitated and pooled before loading onto a NextSeq2000 sequencer (Illumnina) to generate 100 base single end reads (approximately 20M per sample).