GSM6235668: HCC1806_2D_1xSRSF3_3; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNeasy kit (Qiagen) including DNase I treatment per manufacturer instructions. Barcoded RNA libraries were prepared starting with 500ng of total RNA using the TrueSeq stranded mRNA kit with polyA selection (Illumina), and quantified using a Bioanalyzer DNA 1000 chip (Agilent). Libraries were sequenced as 150bp paired-end reads at 100-200 million reads per library on an Illumina NovaSeq, equal amounts of 18 libraries were pooled per lane.