GSM7002177: Heat-triggered ddm1  stem cell nuclei D7, rep1; Arabidopsis thaliana; RNA-Seq
Stem cell nuclei were directly collected for mRNA-seq protocol. Total RNA from seedlings and inflorescence meristems were collected by TRI agent and chloroform. The aquaous phased was collected and then adding 1 volume of isopropanol for RNA precipitation overnight. The pellets were washed  twice with 75% ethanol, then dried and resuspended with RNA-free water. SMART-Seq V2 Illumina NovaSeq SP SR100