GSM7047864: Exp 1 non-targeting sg2 (141); Homo sapiens; RNA-Seq
Total RNA was extracted from on day 20 (biological replicate 1) and 25 (biological replicate 2) post sgRNA transduction using the RNeasy Mini Kit (Qiagen) with on-column DNAse I digestion as per manufacturer's protocol. RNA-Seq libraries were prepared using Roche Kapa mRNA HyperPrep sample preparation kits from 200ng of purified total RNA according to the manufacturer's protocol.