GSM6543590: floxed_spleen_rep3; Mus musculus; RNA-Seq
Total RNA was isolated with the Single Cell RNA Purification Kit (Norgen) following the manufacturer's protocol. RNA-Seq library for mRNA sequencing was prepared with SMARTer Stranded Total RNA-Seq kit - Pico Input Mammalian. All extracted RNA samples were quality controlled for integrity with a 2100 Bioanalyzer system (Agilent Technologies) and were sequenced on a NovaSeq 6000 System (Illumina)