Illumina HiSeq 2000 sequencing; Perturbation of placental endocrine function by endoplasmic reticulum stress leads to maladaptation of maternal hepatic glucose metabolism
RNA Sequencing was performed using BeWo choriocarcinoma cells.  Cells were treated with the ER-stress induce, thapsigargin (concentration is 100 nM. As thapsigargin was dissolved in DMSO and was at 1 mM stock, therefore, 0.01% DMSO was added as vehicle control.) or vehicle in a paired design for 24 hour in serum-free modified DMEM/F12 medium containing 5.5 mM glucose.  This was repeated using 5 independent replicate cultures (so 10 cultures in total).     thapsigargin (Tg) with/without treatment for BeWo cells Nucleic acid extraction protocol: RNeasy Plus Mini Kit (catalog no. 74134; Qiagen) was used to isolate total RNA following manufacturerâ€™s instruction. Illumina TruSeq Stranded mRNA Library Kit