GSM6617292: HEK293FRT, ARID1B, Rep1; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using Branson sonicator ChIP-seq libraries were prepared using the KAPA HTP Library Preparation Kit complemented with NEXTflex DNA Barcodes from Bioo Scientific. Approx 5 ng of DNA was used as starting material for ip samples. Libraries were amplified using 15 cycles on the thermocycler. Post amplification libraries were size selected at 275-700bp in length using 1.5% Pippin cassesstte. Libraries were validated using the HS qubit assay as well as HS BA assays.