GSM5391677: rip_ago2_shints11_24h_noS4U_r2; Homo sapiens; RIP-Seq
RIP was performed in the dark from s4U treated cells at the indicated timepoints. Before RNA extraction, 2 pmol synthetic ath-miR-159a was spiked-in and RNA was extracted using Trizol reagent (Thermo Fisher Scientific, #15596026) according to the manufacturer's instructions. All samples were treated with Iodoacetamide (+IAA) for thiol modification. Genomic DNA was removed by Turbo DNAse treatment (Invitrogen, #AM1907). Small RNA libraries were prepared using the SMARTer smRNA-seq kit (Takara, #635030) with RNA resulting from RIP (typically 200-400 ng)