GSM5819182: Npm1c LT-HSC rep B; Mus musculus; ATAC-seq
Method for extraction was adapted from Buenrostro et al. Nat Meth 2013. Briefly, nuclei were prepared, DNA is transposed and purified followed by PCR amplification. Library quality and concentration were evaluated using the Bioanalyzer High Sensitivity DNA Analysis assay (Agilent Technologies) and quantitative PCR (Kapa Biosystems). ATAC libraries were prepared for sequencing using standard Illumina protocols