GSM6049318: M_baseline_ARS; Mus musculus; RNA-Seq
Five hours after the acute stress, animals were sacrificed and perfused in cold PBS. The PVN was dissected from the fresh brain, and digested in Papain supplemented with DNAse I. After dissociation and filtering, cells were concentrated to 700,000-900,000 cells/ml and loaded on a v2 chip in the 10x Genomics Chromium Controller. Libraries were prepared using the the 10x Genomics Single Cell 3' Reagent Kits v2 according to the manufacturer's protocol.