GSM6588955: Mini-pig-3; Sus scrofa; Bisulfite-Seq
AllPrep DNA/RNA/miRNA Universal Kit (Qiagen, Germany, cat no.: 802224) according to the instructions of the manufacturer RRBS libraries were constructed from 100ng genomic input DNA using the Ovation® RRBS Methyl-Seq library preparation kit (Tecan, cat no.: 0553-32). Briefly, genomic DNA was digested with MspI enzyme, followed by adaptor ligation to Ovation RRBS Methyl-seq adaptors, containing unique molecular barcodes (UMIs), followed by a final end-repair. Ligated DNA was subjected to bisulfite conversion and left in the thermal cycler overnight at room temperature. The following day, bisulfite-converted DNA was amplified by PCR followed by a final clean-up. Sequencing of RRBS libraries was performed on a NovaSeq 6000 platform (Illumina) as 101 bp single-end reads.