GSM6235425: RNAseq, regeneration tips 0hr, DMSO, Rep3; Hydra vulgaris; RNA-Seq
The polyps were decapitated, and the regenerating tips were collected at 0 hpa, 3 hpa, 8 hpa, 12 hpa, and 24 hpa. RNA was isolated using Trizol. The isolated RNA was used to perform 3' mRNA sequencing using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen, Austria) according to the manufacturer's instructions.