Illumina NovaSeq 6000 paired end sequencing; Single cell analysis of emergent haematopoiesis in the human fetal bone marrow
Samples were collected by the Human Developmental Biology Resource and subsequently dissected by their team. Single cells suspensions were made by mechanical digestion and live, enriched cells were selected by FACS. RNA was extracted by cell lysis during droplet encapsulation on board the 10x Chromium Controller and purified after Reverse Transcription using DynaBeads.  Libraries were constructed after cDNA amplification by fragmentation and ligation of adapter sequences before a final index PCR adding sample specific barcodes and sequencing adapters.