GSM6048669: 12week Hetero Heart; Mus musculus; RNA-Seq
Total RNAs from mouse heart were extracted by using RNeasy Plus Universal Mini Kit (Qiagen). Representative libraries were generated for each groups (Wild-type, Heterozygote and Homozygote) by RNA-seq with SMART-seq v4 Ultra Low Input RNA kit for sequencing (Takara Bio Inc.) and Nextera XT DNA Library Prep Kit (Illumina, Tokyo, Japan) and sequenced on a NovaSeq 6000 instrument (Illumina). RNA libraries were prepared for sequencing using standard Illumina protocols.