GSM7047606: WGBS_P900L_F_306-2; Mus musculus; Bisulfite-Seq
DNA was extracted using the Allprep DNA/RNA Mini Kit (Qiagen). 50ng of DNA was sonicated using the S220 Ultrasonicator (Covaris) and subsequently bisulfite converted using the EZ DNA Methylation-Direct Kit (Zymo Research Corporation). WGBS libraries were prepared using the Accel-NGS Methyl-Seq DNA Library Kit (Swift) and complemented with Methyl-Seq Combinatorial Dual Indexing Kit (Swift). Libraries were amplified using 10 cycles on the thermocycler. Post amplification libraries were size selected at 250-450bp in length using SPRISelect beads from Beckman Coulter. Libraries were validated using the Agilent DNA D1000 Screntape Assay Kit.