GSM6588892: Isolated transmural sections from a single explanted ACM heart; Homo sapiens; OTHER
mRNA extraction, barcoding, reverse transcription and in vitro transcription were performed as described (Junker et al., 2014, PMID: 25417113) using the Message Amp II kit (Ambion). See online methods and supplementary of the manuscript. After isolation, we cryosectioned samples, extracted RNA from the individual sections, and amplified and barcoded mRNA using the CEL-seq protocol as in (Junker 2014, PMID: 25417113), but with CEL-Seq v2 reverse transcription primers; See online methods and supplementary of the manuscript. Illumina TRU-seq libraries were constructed CEL-Seq v1 (but with CEL-Seq sample barcodes), as in (Junker et al 2014); See online methods and supplementary of the manuscript. Truseq RNA stranded polyA