GSM5391036: ER-Hoxa9-Lys-GFP (+) E2 (untreated) H3K27ac ChIP; Mus musculus; ChIP-Seq
Formaldehyde fixation, nuclear isolation, probe sonication, ChIP of 500 ug chromatin (for histone modification ChIPs) or 30 ug chromatin (for V5-Hoxa9-AM/V5-ER-Hoxa9-AM ChIPs) with 4-10 ug antibody, cross-link reverasal, proteinase K treatment, and DNA purification via phenol chloroform extraction. NEBNext ChIP-seq Library Master Mix Set for Illumina kit with AMPure XP beads for purification.