GSM5931372: DCM4; Homo sapiens; RNA-Seq
Cells were collected with TRI reagent (AM9728, Life Technologies), stored in −80 °C and transported on dry ice. Total RNA was extracted using RNA Kit according to the manufacturer's recommended procedure. Affymetrix microarray performed by GeneChem (Shanghai Genechem Co., Ltd.) was used for mRNA profiling. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample. Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads.