GSM5931099: Standard_ctr_03; Caenorhabditis elegans; RNA-Seq
Individual worms were mechanically lysed using TissueLyser LT (Qiagen) without pre-processing, followed by RNA extraction using the Rneasy Mini Kit (Qiagen). cDNA synthesis was carried out using the SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio). Sequencing-ready libraries were prepared using the Nextera XT DNA Library Prep Kit (Illumina).