GSM6870619: PBS_ST_3; Mus musculus; RNA-Seq
Total RNA was extracted using RNeasy columns coupled with on-column DNase I digestion (Qiagen) from PEC lavages. The RNA integrity was assessed on a TapeStation (Agilent, Santa Clara, CA, USA). The RNA-seq libraries were prepared using the TruSeq ribodepleted mRNA stranded kit (Illumina, San Diego, CA, USA) and sequenced on a NovaSeq 6000 (Illumina) using a paired-end 100bp configuration aiming for 60 million paired reads per sample.