GSM5509269: Parva WT-1; Mus musculus; RNA-Seq
Isolation of glomeruli form 9 week old male mice with mild proteinuria was performed based on perfusion with magnetic beads (Dynabeads) as previously described in detail (Boerries, Melanie et al. “Molecular fingerprinting of the podocyte reveals novel gene and protein regulatory networks.” Kidney international vol. 83,6 (2013): 1052-64. doi:10.1038/ki.2012.487). RNA was isolated from whole glomeruli using the Qiagen RNAeasy midi Kit (74104, Quiagen) according to the suppliers instructions. RNA libraries were prepared for sequencing using the NEBnext ultra II RNA directional kit. Libraries were sequenced on the Illumina NovaSeq 6000 sequencer following the manufacturer's protocols.