GSM6764946: Day0-30min-BC_B (ATAC-seq); Homo sapiens; ATAC-seq
Immediately following transposition, DNA was purified using a Qiagen MinElute Kit with elution into 10mM Tris buffer, pH 8. Initial amplification of transposed fragments was performed using customized Nextera PCR Primer 1* and  customized Nextera PCR Primer 2* with  NEBNext High-Fidelity PCR mix (New England Labs Cat #M0541) with 8-14 (median 12) cycles of amplification. Amplification was monitored using qPCR and Bioanalyser (Bioanalyzer High Sensitivity DNA kit). Amplified libraries were cleaned up using Qiagen PCR Cleanup Kit and eluted in 10mM Tris Buffer, pH 8 and quantified using KAPA Library Quant Kit for Illumina Sequencing Platforms (KAPABiosystems). Samples were sequenced on a NextSeq500 with 2x76-bp run paired-end reads.