GSM5456236: M2_WT_Input_S18; Mus musculus; RNA-Seq
200-250 M2 eggs or early 2-cell embryos were collected from Eif4e1b with C terminal FLAGHA knock-in female or WT female mice as one sample. After removing the zona pellucida, washed with PBS, embryos for a same sample were transferred into a 1 ml nuclease free centrifuge tube with a minimal volume of PBS. EZ-Manga RIP kit and anti-HA beads were used to perform RIP experiment. RNA in supernatant after IP was purified with RNAclean XP beads and used as input. Smart-Seq2 protocol was used to process RNA bound by anti-HA bead or RNAclean XP beads and finally finish the RNA-Seq libraries.