GSM6311037: Kyse150_shNC_rep1; Homo sapiens; RNA-Seq
Total RNA was isolated by Trizol and mRNA was enriched by Dynabeads Oligo (dT)25 beads RNA libraries for RNA-seq were prepared using the VAHTSTM mRNA-seq V2 Library Prep Kit for Illumina (NR601, Vazyme, Nanjing, China) with 3 independent replicates following manufacturer's protocols.One hundred and fifty bp paired-end sequencing was performing using Illumina HiSeq Xten.