GSM6617007: OSA06, replicate 6, scRNAseq; Homo sapiens; RNA-Seq
PBMCs from subjects' blood samples were isolated by gradient centrifugation using Ficoll-PAQUE (GE Healthcare Pharmacia, Chicago IL), cell viability was verified, and samples were cryopreserved in liquid nitrogen until use. After thawing, quality control of the isolated single-cells was conducted thoroughly to monitor the suitability of each sample before undergoing single-cell sorting and library preparation, as detailed in the Supplemental Material. scRNA-seq libraries were generated from isolated PBMCs using Chromium 3' v3.1 (10X Genomics, Pleasanton, CA), with a targeted output of 10,000 cells per sample. Libraries were sequenced using an NextSeq (Illumina, San Diego, CA) instrument, targeting over 50,000 reads per cell.