GSM6808340: Functional_Biolrep_3; Eublepharis macularius; RNA-Seq
Each  sample contained 3 teeth and the teeth . Tubes were snap frozen in liquid nitrogen. Each sample was disrupted using a glass tissue grinder and a Qiagen Shredder column (ID:79656). Total RNA was extracted using a Qiagen RNeasy micro kit (ID: 74004). Qualifying samples were prepped following the standard protocol for the NEBnext Ultra ii Stranded mRNA (New England Biolabs) at Biomedical research Center sequencing Core at University of British Columbia. Sequencing was performed on the Illumina NextSeq2000 with Paired End 59bp × 59bp reads. Sequencing data was demultiplexed using Illumina's bcl2fastq2. The output was 20 million paired end reads