GSM5602658: red-blood-0h; Mus musculus; Hi-C
Hi-C in cell and tissue was performed following the manufacturer's protocol (A510008, Arima Genomics). Cells were cultured and crosslinked using 1% formaldehyde for 10 minutes and quenched with 0.125M glycine for five minutes at RT. A total of 3 million cells were used for each Hi-C experiment. Tissue samples were frozen with liquid nitrogen and pulverized using mortar and pestle until the sample resembles a fine powder, then crosslinked using the same condition as cell lines. Hi-C libraries were generated and quality checked per manufacturer's protocol. 300-600M reads per each sample was generated based on total usable reads above 20Kb.