GSM5930980: Periodontal ligament cells - batch 1 rep1; Mus musculus; RNA-Seq
Dissected teeth were treated with Liberase TM to obtain a PDL cell suspension Cells were sorted into 384-well plates with barcoded oligo dT primer for each well (1 cell / well). After reverse transcription in each well, cDNA was pre-amplified using the Accel-NGS 1S Plus DNA Library Kit (Swift Biosciences). Purified cDNAs were subjected to tagmentation reaction with Amplicon Tagment enzyme (Illumina), and then amplified by PCR for library construction using NEBNext High-Fidelity 2× PCR Master Mix (NEB).