GSM6544057: RNAseq_BT869_Compound11_24h_biolrep2; Homo sapiens; RNA-Seq
Total RNA was extracted from frozen cell pellets using the Qiagen RNeasy mini plus kit (Cat# 74134), and RNA concentrations were determined using a NanoDrop spectrophotometer (Thermo Scientific). Library preparation and sequencing was performed by Novogene. In brief, messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries were pooled and sequenced on Illumina platforms, according to effective library concentration and data amount. The original raw data from Illumina platform were transformed to Sequenced Reads, known as Raw Data or RAW Reads, by base calling. Raw data were recorded in a FASTQ file, which contained sequencing reads and corresponding sequencing quality.