GSM5931622: CaMKK2KO; Mus musculus; RNA-Seq
Immune tumor infiltrates were isolated using FACS sorting. Single-cell suspensions were loaded on the 10x Genomics Chromium controller, and mRNA converted to cDNA lirbaries. Single-cell libraries were sequenced on a Novaseq 6000 150PE S-Prime flowcell. Libraries were sequenced in single index mode with the following read lengths: 28x8x91 10x Chromium Single Cell 3' Kit using the v3.1 chemistry