GSM6766356: 1 Control-001; Homo sapiens; RNA-Seq
Cells were washed with PBS then each plate containing cells was frozen at -80 °C until further collection of RNA. RNA and DNA were simultaneously isolated from the indicated clones from plates 1-4  using AllPrep DNA/RNA Kit (#catalogue: 80284, Qiagen) according to manufacturer's protocols. RNA extracted from cell lines was quantitated with the Qubit Fluorometer (ThermoFisher Scientific, Waltham, MA) and screened for quality on the Agilent TapeStation 4200 (Agilent Technologies, Santa Clara, CA) The samples were then processed for RNA-sequencing using the NuGEN Universal RNA-Seq Library Preparation Kit with NuQuant (NuGEN Technologies, San Carlos, CA) The final libraries were normalized, denatured, and sequenced on the Illumina NextSeq 2000 sequencer with the P3-200 cycle reagent kit in order to generate approximately 50M million 100-base read pairs per sample (Illumina, Inc., San Diego, CA)