GSM5971448: GBMEC5391; Homo sapiens; RNA-Seq
2 x 10^6 ECs were lysed in 1 ml TRIzol (Thermo Fisher Scientific), followed by RNA extraction according to the manufacturer's instructions. The isolated RNA was purified with RNeasy Plus Mini Kit (Qiagen). After a quality control step using RNA Nano assay chips with a 2100 Bioanalyzer (Agilent), a library was constructed using a Ribo-Zero protocol with TapeStation (Agilent) and subjected to deep sequencing (125 paired-end (PE); approximately 40 MB reads for each sample; Illumina HiSeq 2500 sequencer) at the Children's Hospital of Philadelphia.