Ion Torrent PGM sequencing; Targeted next generation sequencing of genomic DNA from PLC, JEG3 and JEG3R cells
Cells were pelleted at 300 x g and stored at -80C until DNA extraction DNA was extracted using a DNeasy kit (QIAGEN) Library generation followed the protocol described in the Ion AmpliSeq Library Kit 2.0 User Guide using the Ion AmpliSeq Cancer Hotspot Panel v2 primer pool, comprising 207 amplicons covering approximately 2,800 COSMIC mutations from 50 oncogenes and tumor suppressor genes.