GSM6235083: B16F10 cells, anti-PD-1 + PBS, MEL_58; Mus musculus; RNA-Seq
RNA was harvested using TRIzol Reagent(Ambion/Invitrogen). 1.0 ug of total RNA was used for the construction of sequencing libraries. Library construction protocol RNA libraries for RNA-seq were prepared using NEBNext Ultra RNA Library Prep Kit for Illumina following manufacturer's protocols.