GSM6617892: LAL-B_Rep3_ATACseq; Homo sapiens; ATAC-seq
ATACseq : The nuclei were centrifuged at 500g for 5 minutes at 4°C and subsequently resuspended on ice in 50μl transposase reaction buffer containing 2.5μl of Tn5 transposase and 25μl of 2xTD buffer (Nextera DNA Sample preparation kit from Illumina). After incubation at 37°C for 30 minutes, the samples were purified with MiniElute PCR Purification Kit (Qiagen), eluting in 10µl elution buffer (10mM Tris-HCl pH 8). ATACseq's  libraries were purified by adding Agencourt Ampure XP (Beckman) magnetic beads (1:1 ratio) to remove remaining adapters (left side selection) and double purified (1:0.5 and 1:1.15 ratio) for right side selection. Libraries were controlled using a High Sensitivity DNA Kit on a Bioanalyzer (Agilent Technologies). Each library was then paired end sequenced (2×75bp) on a NextSeq 500 instrument (Illumina).