Illumina HiSeq 4000 sequencing; Unraveling Transcriptomic Heterogeneity in the Human Dermal Blood Vascular Endothelium at Single-Cell Resolution
Endothelial cells were isolated from healthy adult skin samples. Cells were sorted into 384-well plates containing lysis buffer and RNA was extracted. Libraries were prepared using a miniaturised version of the Smart-seq2 protocol (Picelli et al., 2014).