GSM6314874: M28z-KITv CAR-T cells stimulated for 24 hours_rep 2; Homo sapiens; RNA-Seq
RNA was extracted using the RNeasy Mini Kit (QIAGEN), according to the instructions provided by the manufacturer. After RiboGreen RNA quantification and quality control using an Agilent Bioanalyzer. libraries were prepared using TruSeq RNA Library Prep Kit v2 (Illumina), followed by sequencing (60 million 50 bp paried-end reads) on Illumina NovaSeq 6000.