GSM5818768: ChIPseq_H3K9me3_WT_hMSC_R1; Homo sapiens; ChIP-Seq
Lysis of 5 X 10 ^6 fixed cells was sonicated with Diagenode Bioruptor Pico (HKU, Imaging & Flow Cytometry Core facility) for 20 cycles to generate DNA fragments ranging from 200-1000 bp and discarded insoluble part after 15,000 RPM for 20min. The chromatin supernatants were treated as described in PMID: 17406303 and ChIP libraries were prepared by NEBNext® ΜltraTM DNA Library Prep Kit for Illumina® (NEB E7370). Information of antibodies used in ChIP: CTCF (Rb, Active motif 61311), H3K27ac (Rb, Active motif 39034), H3K27me3 (Rb, Abcam ab6002), H3K9me3 (Rb, Abcam ab176916), Lamin A/C (Ms, Active motif 39287) and Lamin B1 (Rb, CST 13435S). ChIP