GSM6724131: Control, scRNAseq; Mus musculus; RNA-Seq
Briefly, cortex samples were digested in papain (20 units/ml) for 45 minutes at 37 °C. After stopping the dissociation with ovomucoid, the cells were resuspended in PBS with 0.04% BSA. Cells were counted and viability was checked using Trypan Blue. scRNA-seq libraries were generated using the 10x Genomics Chromium Single Cell 3′ Reagent Kit v3 according to the manufacturer's protocol and sequenced on an Illumina NovaSeq6000 S2 200 flow cell at the Genomics Core Technology Unit (GCTU), Queen's University Belfast.