GSM6543215: ATACseq_BT869_AAVS1ko_d4; Homo sapiens; ATAC-seq
40,000-50,000 live cells were sorted by FACS and lysed in 0.1% NP40 (Sigma/Roche 11332473001), 0.1% Tween-20 (Sigma/Roche 11332465001), and 0.01% digitonin (Promega, G9441), followed by tagmentation with 100 nM Tn5 transposase (Illumina, 15027865) for 30 min at 37 °C. Tagmented DNA was purified using the MinElute PCR Cleanup kit (Qiagen) and PCR amplified using NEBNext-Fidelity 2X PCR Master Mix (NEB M0541S) and primers containing Illumina adapter sequences. Final library was purified by MinElute PCR Cleanup kit (Qiagen). Libraries were prepared and sequenced in two technical replicates on a Nextseq 500 sequencer (Illumina) using Nextseq High Output Cartridge kits by paired-end reads.