GSM7047853: ZR01-Ja2kVF+EV-m2; Mus musculus; RNA-Seq
Total RNA was extracted from sorted Lineage- Sca1+ Kit+ (LSK) cells using Arcturus PicoPure RNA Isolation Kit (Applied Biosystems) Following RNA isolation and quantification, 5 ng of total RNA was used to prepare the cDNA libraries as per the manufacturer's protocol (NEB Next Ultra II RNA Library Prep Kit for Illumina, New England BioLabs). Briefly, polyA RNA was isolated, fragmented and primed prior to cDNA synthesis, end repair/dA tailing and Illumina adaptor ligation. The resulting products were PCR enriched /indexed with size selection and quantitation before sequencing.