GSM5952553: PL-2 control camel supraoptic nucleus; Camelus dromedarius; RNA-Seq
Unilateral supraoptic nucleus samples were isolated from hypothalamus of male dromedary camels under control (water ad libitum) and dehydrated (water deprivation for 20 days) conditions (n=5 per group). Total RNA was extracted using a Direct-zol™ RNA MiniPrep kit (Zymo research, R2052) following the manufacturer's instructions. Average RNA Integrity Number (RIN) of samples processed for sequecing (n=10) was 6.27. Ribosomal depleted sequencing libraries were produced using TruSeq® Stranded Total RNA library preparation kit (illumina). Libraries were sequenced on Illumina NextSeq500 platform using NextSeq 500 High Output v2.5 (2 x 75 bp run) Kit (Illumina; 20367064).