GSM6766698: TrtdD5; Mus musculus; RNA-Seq
Single cell was sorted using CellenONE into NanoPOTS wells containing 0.1% DDP in 10 mM Tris pH8 For RNA‐Seq, TheSMART-seq V4 (cat#R400753) was used to generate cDNA library for illumina NextSeq 550 platform according to the manufacture protocol. Single-read sequencing of the cDNA libraries with a read length of 150 was performed with NextSeq 500 Sequencing System using NextSeq 500/550 High Output v2 kit 150 cycles (cat#20024907).