GSM5907967: fetal pancreas pool_3_131419_PCW_Gene expression; Homo sapiens; RNA-Seq
Human fetal pancreases were dissociated into single cells by collagenase and trypsin/EDTA treatment. Each batch contains 3 samples from different gestational stages which were stained with oligonucleotide-conjugated antibodies from BioLegend and pooled Single cell mRNA and antibody (HTO) libraries were prepared according to the manufacturer's protocol and paired-end sequencing was carried out on an Illumina NextSeq 2000, except for batch 15, 18 and 20 PCW batch which was sequenced on a HiSeq 2500.