GSM5907504: Control1; Sus scrofa; RNA-Seq
The specimens were homogenized using Precellys lysing kit CK28 beads (VWR, Vienna, Austria) in Qiazol lysis buffer (Qiagen, Hilden, Germany) and total RNA was isolated using the RNeasy® Tissue kit (Qiagen, Hilden, Germany). The total RNA fractions of the myocardial samples were subjected to mRNA deep sequencing using the Illumina (San Diego, CA, USA) platform. The NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Ipswich, MA, USA) was utilized for mRNA fragmentation and enrichment. Fragmented and primed mRNA was reverse transcribed to cDNA. The cDNA library was synthesized and enriched using the NEBNext Ultra Directional RNA Library Kit (New England Biolabs, Ipswich, MA, USA). Sequencing was performed on the HiSeq 2500 platform to a mean depth of 15–20 million paired-end reads per sample at the Core Facility of the Medical University of Vienna.