Illumina NovaSeq 6000 paired end sequencing; RNA-seq of whole zebrafish embryos at 6 and 10hr post fertilisation
Zebrafish embryos (obtained from wild-type strain AB) were collected at the shield stage and tail-bud stage, which correspond to 6 hpf and 10 hpf, respectively.  Total RNA was extracted with miRNeasy Mini Kit (Qiagen, 217004) according to the manufacturer's instructions. Quality of extracted RNA was assessed using the NanoDrop ND-1000 Spectrophotometer (ThermoFisher Scientific) and by agarose gel electrophoresis. Samples were stored at -80 ℃ Libraries for sequencing were prepared with TruSeq stranded mRNA library prep kit (Illumina) according to the manufacturer's instructions.