GSM5931279: Seq04_SingleCell; Mus musculus; RNA-Seq
Cells were stained for flow cytometry and and sorted. The sorted cells were pooled, spun down and resuspend in sample buffer. Cell counting was performed using an INCYTO hemocytometer, and a total of 58,000 cells were loaded on a BD Rhapsody cartridge. Libraries were prepared using the BD Rhapsody mRNA Whole Transcriptome Analysis and Sample Tag Library Preparation Protocol, as per the manufacturer's recommendations (BD Biosciences).