GSM6589303: heart, RNA pol II, activemotif, WT, rep2; Mus musculus; ChIP-Seq
400 snap-frozen E10.5 heart samples were cross-linked for 10 min in 1% ChIP-seq–grade formaldehyde and washed in 1X PBS and then dispersed into a single cell suspension using a Dounce homogenizer (Fisher scientific # 06–435A). The product was sonicated, and the DNA sheared to fragments of 300 to 500 base pairs. DNA libraries (Vazyme, Cat #ND607-02) were prepared from the CHIP and input DNA using the standard consecutive enzymatic steps to end polishing, dA addition and adaptor ligation using Active Motif's custom liquid handling robotics pipeline. After the final 15-cycle PCR amplification step, the resulting DNA libraries were quantified (concentration > 10 nM) and sequenced with PE150 sequencing on the Illumina Novaseq sequencer (Illumina)