GSM5931598: Control replicate 2; Bos taurus; RNA-Seq
Seven groups of blastocysts were collected on 8 dpi (control, NC mimic, NC inhibitor, miR-378 mimic, miR-378 inhibitor, miR-146b mimic, and miR-146b inhibitor). For each group, total RNA was isolated from three replicates of 10 blastocysts using the RNeasy Micro kit (Qiagen) according to the manufacturer's protocol. The quality and concentration of the RNA samples were examined using an RNA 6000 Pico Chip (Agilent Technologies) and a Quant-iT RiboGreen RNA Assay kit (Life Technologies), respectively. Transcriptome library preparation was done by Qiaseq UPX 3' transcriptome kit (Qiagen)