GSM5509266: Sample C; Homo sapiens; RNA-Seq
Once the inflammatory periapical tissue of the three teeth was carefully scraped off, the collected samples were immediately placed in an ice-cold preservation solution and then transported to the laboratory to maintain viability. Using the manufacturer's protocol (10x Single Cell 3' Reagent Kits v2 manual, USA) (10X Genomics, 2016), the prepared cell suspensions were loaded onto barcoded scRNA-seq. In brief, the generation of gel beads in emulsions (GEMs), barcoding, GEM-RT clean-up, complementary DNA amplification and library construction were performed.