GSM6543914: KYSE150-Luc-LM3 cells; Homo sapiens; ncRNA-Seq
A total amount of 3 μg RNA per sample was used as initial material for the RNA sample preparations. Subsequently, the sequencing libraries were generated following manufacturer recommendations with varied index label by NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (NEB, Ispawich, USA) . RNA libraries for lncRNA-seq were prepared using ruSeq PE Cluster Kit v4-cBot-HS (Illumina)  following manufacturer's protocols.