GSM5509268: PBMC; Homo sapiens; RNA-Seq
Total RNA was extracted from bone marrow mononuclear cells and peripheral blood mononuclear cells and digested with DNase I (TIANGEN Biotech) to remove contaminating genomic DNA. Equal amount of total RNA extracted from the patient BM and peripheral blood was used as template to perform dimer-avoided-multiplex PCR according to the manufacturer's protocol (iRepertoire Inc.). PCR products were purified and sequenced (Illumina Nova6000). RNA seq (iRepertoire)