GSM6311324: PNAC-1 cells, RSL3, 12h, rep2; Homo sapiens; RNA-Seq
The total RNA was extracted from PANC-1 cells treated with or without RSL3 (n=3 per group) using TRIzol (Invitrogen, 15596018). Purity and concentration of RNA were determined with NanoDrop spectrophotometer (Thermo Scientific), and integrity was determined by Agilent 2100 bioanalyzer (RNA 6000 Nano kit 5067-1511). Sequencing libraries were generated using SMARTER mRNA-Seq Library Prep Kit according to manufacturer's protocols.