GSM5818753: ChIPseq_Input_WT_hMSC_R2; Homo sapiens; ChIP-Seq
Lysis of 5 X 10 ^6 fixed cells was sonicated with Diagenode Bioruptor Pico (HKU, Imaging & Flow Cytometry Core facility) for 20 cycles to generate DNA fragments ranging from 200-1000 bp and discarded insoluble part after 15,000 RPM for 20min. Input material was reserved. ChIP