GSM6241146: N2_emb_rep1 [DJ73]; Caenorhabditis elegans; Hi-C
Hi-C: Crosslinked worms were resuspended in 20 ul of PBS per 50ul of worm pellet then dripped into liquid nitrogen containing mortar. The worms were grounded with pestle until fine powder. Grounded worms were crosslinked again in 2% formaldehyde using the TC buffer as described by the Arima Hi-C kit, which uses two 4-base cutters, DpnII and HinfI. The Arima manufacturer's protocol was followed. Hi-C:  Library preparation was performed using the KAPA Hyper Prep Kit using the protocol provided by Arima.