GSM6542801: Sertoli,DMRT1, Santa; Capra hircus; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a E220 focused-ultrasonicator (Covaris). The 10% lysis sonicated chromatin was stored and named “Input”, and 80% was used in immunoprecipitation reactions with specific anti-Flag Magnetic Beads (Beyotime Biotechnology, P2115) and named “IP”, and 10% was incubated with rabbit IgG (Cell Signaling Technology) as a negative control and named “IgG”，respectively. The DNA of Input and IP was extracted by phenol-chloroform method. The high-throughput DNA sequencing libraries were prepared by using VAHTS Universal DNA Library Prep Kit for Illumina V3（Catalog NO. ND607, Vazyme）.