GSM6593379: mouse-epidermis-fresh-A; Mus musculus; Bisulfite-Seq
Geonomic DNA extracted with DNeasy Blood and Tissue kit from Qiagen 100 ng genomic DNA was fragmented to 350 bp with the Covaris S220 Focused-ultrasonicator (Covaris). DNA was bisulfite treated using the EZ DNA Methylation-Gold™ Kit (Zymo Research). Whole genome bisulfite sequencing (WGBS) libraries were prepared using the Accel-NGS® Methyl-Seq DNA Library Kit (Swift Biosciences) following the recommended instructions and five PCR cycles for the final library amplification.