GSM5818723: SVI-030-18; Homo sapiens; RNA-Seq
Pancreas following partial pancreatectomy was obtained, with informed consent following research approval from the Human Research Ethics Committee at St. Vincent's Hospital Melbourne. Human islets were isolated by intraductal perfusion and digestion of the pancreas with collagenase followed by purification using ficoll density gradients. Islets were dispersed with accutase then rested in Connaught Medical Research Laboratories (CMRL) 1066 medium (Invitrogen) supplemented with 4% human serum albumin, 100 U/ml penicillin, 100 mg/ml streptomycin and 2 mM L-glutamine (complete CMRL), in a 37°C, 5% CO2 humidified incubator for 2 hours. Cells were then processed for single cell RNAseq using the Chromium 10x genomics platform and sequenced using the Illumina NextSeq platform at the Australian Genome Research Facility (Melbourne, Australia)