GSM6543705: ChIPseq_BT869_SMARCA4ko_Input; Homo sapiens; ChIP-Seq
Cell pellets were frozen at -80C, and sent to Active Motif Services (Carlsbad, CA) to be processed for ChIPseq. Cells were fixed with 1% formaldehyde for 15 min and chromatin was sheared sheared to an average length of 300-500 bp. Illumina sequencing libraries were prepared from the ChIP and input DNAs by the standard consecutive enzymatic steps of end-polishing, dA-addition, and adaptor ligation. Steps were performed on an automated system (Apollo 342, Wafergen Biosystems/Takara). After a final PCR amplification step, the resulting DNA libraries were quantified and sequenced on Illumina's NextSeq 500 (75 nt reads, single end).