GSM5695709: NBL-S H3K27me3; Homo sapiens; ChIP-Seq
Chromatin immunoprecipitation, formaldehyde cross-linking of cells, cell lysis, sonication and library preparation were performed as described previously (Blecher-Gonen, R. et al. High-throughput chromatin immunoprecipitation for genome-wide mapping of in vivo protein-DNA interactions and epigenomic states. Nature protocols 8, 539-554, doi:10.1038/nprot.2013.023 (2013)). Approximately 4 × 106 cells (1 × 106 cells per individual immunoprecipitation) were used and cell lysis for each sample was achieved by 30 min incubation on ice in 950 µl RIPA I (10 mM Tris-HCl (pH 8.0), 1 mM ethylenediaminetetraacetic acid (pH 8.0), 140 mM NaCl, 0.2% sodium dodecyl sulfate and 0.1% sodium deoxycholate (DOC)). Library preparation was performed using the NEBNext Ultra DNA Library Prep Kit (New England Biolabs) according to the manufacturer's protocol. Samples were mixed in equal molar ratios and sequenced on an Illumina sequencing platform.