GSM6266309: KeaA-HA_input_biol_rep_1; Aspergillus nidulans; ChIP-Seq
ChIP was conducted according to Boedi et al., 2012 (Meth. in Mol. Biol., vol.  944, p.221-236; Springer Protocols). 1% formaldehade for 15 min was used as crosslinking agent, mycelia were grinded in mortar, extracts were sonicated using Bioruptor 200 (Diagenode) with 2 min "on"/1 min "off"/power "high" settings for 25 min and anti-HA 12CA5 antibody was used for chromatine immunoprecipitation (Roche, #11583816001). ChIP-seq libraries were prepared using KAPA HyperPrep Kit and KAPA Dual-Indexed adapters (Kapa Biosciences) with 10 cycles of amplification. The size distribution was assessed using the Agilent 2100 Bioanalyzer and High Sensitvity DNA kit. The concentration of the libraries was determined by qPCR using the Kapa Library Quantification kit (Kapa Biosciences) and Roche LC480II cycler. Sequencing was performed on the Illumina NovaSeq 6000 instrument using the NovaSeq 6000 S2 Reagent Kit (100 cycles) (Illumina), with 2 × 100 cycles pair-end reading mode.