GSM5456023: shNC_input; Homo sapiens; RIP-Seq
total RNA was extracted using Trizol reagent, and poly (A) mRNA was purified from 100 μg total RNA  using a Dynabeads mRNA kit. immunoprecipitated mRNAs   or Input was used for library construction with NEBNext ultra RNA library prepare kit  for Illumina (New England Biolabs).