GSM6617836: 1241 ZEB2 Input; Homo sapiens; ChIP-Seq
Post-mortem brain tissue was homogenized and fixed for 10 minutes at room temperature, followed by 5 minutes of quenching with Glycine. Fixed nuclei were isolated by ultracentrifuging 24,000 RPM for 2 hours  at 4C. If sorted, nuclei were stained for NeuN/OLIG overnight and sorted the next day. Sorted nuclei were pelleted and resuspended in RIPA for 8 rounds of sonication using the Diagenode Bioruptor Pico. Libraries were prepared by blunting and ligating ChIP DNA fragments to sequencing adapters for amplification with barcoded primers (30 sec at 98°C; [10 sec at 98°C, 30 sec at 65°C, 30 sec at 72°C] x 15 cycles; 5 min at 72°C). Libraries were quantified with Qubit dsDNA HS Assay kit and visualized with Standard Sensitivity NGS Fragment Analysis Kit (Advanced Analytical DNF-473) and Fragment Analyzer 5200 (Agilent).