GSM5509842: RBBP5FKBP_8h_TT_seq_Rep2; Mus musculus; OTHER
Following the specified treatment, we supplemented the treatment medium with 1 mM 4-thiouridine (4sU) and metabolically labeled the cells for 10 min. The cells were lysed in QIAzol (Qiagen, 79306) and total RNA was isolated according to the manufacturer's instructions before the addition of 100 ng RNA spike-in mix together with QIAzol. Libraries were constructed using TruSeq Stranded Total RNA kit (Illumina, 20020596).