GSM5932045: R2-post; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNeasy FFPE Kit. RNA sequencing libraries were prepared using KAPA Stranded RNA-Seq Kit with RiboErase (KAPA Biosystems). rRNA was first depleted with RiboErase, followed by DNase digestion for DNA removal. Purified RNA was subjected to first-strand cDNA synthesis, followed by second-strand synthesis with dUTP marking for strand specificity. This is followed by A-tailing, adapter ligation, and library amplification. Final library was  quantified using KAPA Library Quantification Kit (KAPA  Biosystems), and its fragment size distribution was analyzed  using a Bioanalyzer 2100 (Agilent).