GSM6543891: FMSV223; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using  Qsonica 700 sonicator supplied with cup horn 431C2  with parameters:  60% amplitude for 8 minutes, 20 seconds on, 20 seconds off. Sequencing libraries were prepared using NEBNext Ultra DNA Library Prep Kit for Illumina (E7645S; NEB). We used 3 ng DNA for library preparation, and 15 cycles were used for library amplification. Sequencing was performed on an Illumina HiSeq4000 sequencer using a single-end 50-base read in multiplexed mode