GSM7047550: Cold_3h_Rep1; Manihot esculenta; RNA-Seq
Total RNA was isolated using the RNeasy Plant Mini Kit (Qiagen) and treated with Turbo DNase (ThermoFisher Scientific) according to manufacturers' instructions. DNase-treated RNA was recovered by acid–phenol extraction and ethanol precipitation. The libraries were prepared from total RNA samples using Lexogen QuantSeq 3' mRNA-seq REV kit following manufacturer's protocol.