GSM5970948: Dentate Gyrus Differentiation - DIV28 - Rep 1; Homo sapiens; RNA-Seq
All in vitro samples used for transcriptomic assays were harvested by 5', 37oC trypsinization after which was inactivated with inactivated Fetal Bovine Serum (Euroclone ECS0180L). Detached cells were homogeneously suspended in solution, collected in microcentrifuge tubes and pelleted at 200 g for 5'. After centrifugation, the supernatant was aspirated, and the cell pellet was disrupted and processed using NucleoSpin® RNA kit (Machery-Nagel, 740955.250). RNA concentration was measured using a NanoDrop™ Lite Spectrophotometer (ThermoFisher ND-LITE). RNA-seq libraries were prepared with the SMART-Seq® HT PLUS Kit (Takara) as described in manufacturer's instructions