GSM6891990: exp set1, Mpknox2-1 Mpez1-1, 14d thalli, biol rep2; Marchantia polymorpha; RNA-Seq
Total RNA was extracted using a Spectrum Plant Total RNA kit (Sigma Aldrich), 1 ug of total RNA was used for the construction of sequencing libraries. PolyA RNA was selected using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs), then RNA-seq libraries were generated using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs).