GSM6543241: (HK-2 cells, shControl, Day10 1; Homo sapiens; RNA-Seq
Total RNAs were extracted from HK2 cells using TRIzol Reagent following the methods by Chomczynski(Chomczynski and Sacchi, 1987). 2 μg total RNAs were used for stranded RNA sequencing library preparation using KCTM Stranded mRNA Library Prep Kit for Illumina  following the manufacturer's instruction. PCR products corresponding to 200-500 bps were enriched, quantified and finally sequenced on Novaseq 6000 sequencer (Illumina) with PE150 model. PCR products corresponding to 200-500 bps were enriched, quantified and finally sequenced on Novaseq 6000 sequencer (Illumina) with PE150 model.