GSM5456324: MI22-10 uM-1; Homo sapiens; RNA-Seq
Total RNA was isolated and DNase I treated using Qiagen RNeasy Plus Mini Kit (Qiagen, Valencia, CA). The RNA integrity was checked by Agilent Bioanalyzer 2100 (Agilent, Santa Clara, CA) and samples with clean rRNA peaks (RIN>6) were used for library preparation. RNA-Seq library was prepared according to KAPA mRNA HyperPrep kit with 200-300 bp insert size (Roche, Wilmington, MA) using 250 ng of total RNAs as input. Final library quality and quantity were analyzed by Agilent Bioanalyzer 2100 and Life Technologies Qubit 3.0 Fluorometer (Thermo Fisher Scientific Inc, Waltham, MA). 150 bp PE (paired-end) reads were sequenced on Illumina HiSeq sequencer (Illumnia Inc., San Diego, CA).