GSM5509224: DB_N2_VDJ; Homo sapiens; RNA-Seq
FACS-sorted LMP2 multimer-positive cells were harvested. The targeted cell recovery was set at 6000 cells and RNA was isolated using 10X Genomics reagents. Libraries were prepared using the kits Chromium Next GEM Single Cell 5' Kit v2 and Chromium Single Cell Human TCR Amplification Kit (10x Genomics inc.). Libraries were quantified using the Kapa Illumina GA with Revised Primers-SYBR Fast Universal kit (Kapa Biosystems). Average size fragment was determined using a LabChip GX (PerkinElmer) instrument. The 10X Single cells 5' libraries were sequenced on Illumina HiSeq 4000 PE28x98 while the 10X Single Cells VDJ (Human T) libraries were sequenced on Illumina HiSeq 4000 PE150.