GSM5931639: Low OD= 1.0 rep3; Escherichia coli; RNA-Seq
RNA was extracted by applying the RNAeasy Mini Kit (Qiagen cat. 74104) and followed by the DNase   treatment (Qiagen cat. 79254). Total RNA quality and integrity were assessed using the 5200   Fragment Analyzer System (Agilent Technologies). By applying NEBNext® Ultra™ II   Directional RNA Library Prep Kit (New England BioLabs), total RNA library was generated from   1 mg total RNA after rRNA depletion through Ribo-off rRNA Depletion Kit (Bacteria) (Vazyme   BioTech Co.Ltd.). RNA libraries were prepared for sequencing using standard Illumina protocols