GSM6617905: LALB-K27Ac; Homo sapiens; ChIP-Seq
Chromatin immunoprecipitation (ChIP) assays were performed as previously described (Bruno T, Cancer Cell, 2002) by using anti-acetyl-Histone H3 (Lys27) antibody (Millipore). Equal amounts of precleared chromatin were added to antibody-bound Dynabeads (Thermo Fisher Scientific). About 6 ng of the immunoprecipitated chromatin was used to prepare the libraries for sequencing by following the manufacturer's instructions (Swift Bioscences, Cat. No. 21024). ChIP: The final libraries were controlled on an Agilent 2100 Bioanalyzer (Agilent Technologies) and sequenced in paired-end mode (2×75 bp) with NextSeq 500 (Illumina, CA).