GSM5971040: HR07 (RNA-seq); Homo sapiens; RNA-Seq
A PureLink FFPE Total RNA Isolation Kit (Thermo Fisher Scientific) was used for total RNA extraction from diagnostic FFPE tissue. Purified RNA was quantified on a Qubit 3 Fluorometer (Thermo Fisher Scientific) using Qubit™ RNA BR Assay Kit (Thermo Fisher Scientific), and subjected to Total RNA sequencing using the Illumina TruSeq Stranded Total RNA protocol with at least 500 ng of total RNA. The Illumina Total Stranded RNA protocol was applied.