GSM5388441: Cerebellar tissue - 84Q+IGF1_rep2 [22_1_1]; Mus musculus; RNA-Seq
Total RNA was extracted using Trizol® Reagent (Invitrogen, USA) according to the instruction manual. Purified RNA was quantified at OD260nm using a ND-1000 spectrophotometer (Nanodrop Technology,USA) and qualitated by using a Bioanalyzer 2100 (Agilent Technology, USA) with RNA 6000 LabChip kit (Agilent Technology, USA). Samples with very high RNA integrity numbers were selected for library construction, and the libraries were constructed using Agilent's SureSelect Strand-Specific RNA Library Preparation Kit followed by AMPure XP beads (Beckman Coulter, USA) size selection. Libraries were sequenced as paired-end 150 bp reads on an Illumina HiSeq 2500 (Illumina, San Diego, USA) with a depth of ~20 million reads each. Sequencing data (FASTQ reads) were generated using Welgene Biotech's pipeline based on Illumina's base-calling program bcl2fastq v2.20.