GSM5456270: RFP_positive_rep2; Saccharomyces cerevisiae; RNA-Seq
Total RNA was extracted using a hot acid phenol method following spore lysis with freezing and vortexing with garnet and zirconium beads. 100 ng total RNA was used as input for library preparation with a Tecan Universal Plus mRNA-seq Library Preparation Kit with NuQuant (Cat # 0520)