GSM6765807: WT [scRNA-seq]; Mus musculus; RNA-Seq
Single cell suspensions were loaded on a Next GEM Chip G (PN-1000120) together with Next GEM Single Cell 3ʹ GEM Kit v3.1 (PN-1000121) and single cells were captured on a 10X Genomics Chromium controller. cDNAs were generated following the 10X Genomics protocol. cDNAs were size selected using SPRIselect beads from Beckman Coulter (B23318), and their quality was checked with a Bioanalyzer High Sensitivity DNA Kit from Agilent (5067-4626). One quarter of the total cDNA was used to generate libraries using Chromium Next GEM Single Cell Library Kit (PN-1000121) and barcoded using the Single Index Kit Set A (PN-111213) following the 10X Genomics protocol. Libraries were size selected using SPRIselect beads and their quality was checked with the Bioanalyzer High Sensitivity DNA Kit. read1=28 ; i7=10 ; i5=10 ; read2=90