GSM5695854: untreated [PM_0_3]; Mus musculus; RNA-Seq
The total RNA from the GC-2spd cells was isolated using TRIzol reagent. A total amount of 1 µg RNA per sample was used for the RNA sample preparations, and Sequencing libraries were structured with NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumia). After cluster generation, the library preparations were sequenced on an Illumina Novaseq platform and 150 bp paired-end reads were generated.