GSM6808169: MNase-Seq Replicate2_ISW2; Saccharomyces cerevisiae; MNase-Seq
The chromatin was reverse cross-linked with the addition of 1 mg/mL Proteinase K (Life Technologies) and 0.5% SDS at 65ºC for ≥3 hours. For DNA extraction, the reaction was supplemented with glycogen and subjected to ethanol precipitation. Approximately 15-20 ng of each library was indexed using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) using the manufacturer's protocol.