GSM5907987: T_3; Camelus bactrianus; miRNA-Seq
Total RNA was extracted from the plasma-derived exosomes of thin camels and normal camels using mirVANA miRNA Isolation kit (Takara Biomedical Technology Co., Ltd., Beijing, China) according to the manufacturer's recommendations, and then the quality and concentrations of the total RNA were assessed using a microplate reader (OD260/280). After that, the total RNA was sent to OBiO Technology (Shanghai) Corp., Ltd (China) for small RNA sequencing. Approximately 5 µg of total RNA were used to prepare nine small RNA librariesaccording to the protocol of TruSeq Small RNA Sample Prep Kits (Illumina, San Diego, CA, USA). And then the libraries were sequenced by Illumina Hiseq 2500 at the LC-BIO following the vendor's recommended protocol. Raw reads were subjected to an in-house program, ACGT101-miR (LC Sciences, Houston, Texas, USA) to remove adapter dimers, junk, low complexity, common RNA families (rRNA, tRNA, snRNA, snoRNA) and repeats.