GSM5390742: 36O_2; Arabidopsis thaliana; ChIP-Seq
The chromatin extraction and in vitro double crosslinking were performed as previously described (Milhindos et al., 2019). Chromatin was then sonicated using a Bioruptor (Diagenode) for 9 cycles (30''on/30''off each). A 100 µL aliquot of each sample and replicate were kept as input. For immunoprecipitation, samples were incubated wit anti-GFP antibody (ab290; Abcam) and magnetic DynabeadsTM Protein A (ThermoFisher Scientific). Finally, chromatin was eluted, proteins were digested with Proteinase K, and DNA was purified with phenol:chloroform:isoamyl alcohol and precipitated with ethanol.