GSM6588508: M53 Organoid WNTinb 1 day; Mus musculus; OTHER
Tumor organoids were grown in 24 well plates, and after drug treatment for 24 hours, were collected in Cell Recovery Solution (Corning). Tumor organoids were left to rotate at 4° for one hour in order to dissolve the Matrigel. Tumor organoids were spun down at 300 g at 4° for 5 minutes. 100,000 cells per condition were counted and washed with PBS. Nuclei were prepared using Epicypher's CUTANA kit and slowly frozen in nuclear extraction buffer. Epicypher CUTANA kit