GSM7086424: VmSC5.8d.ADM#3; Mus musculus; RNA-Seq
For freshly-isolated samples, 1000 cells were double-sorted into 5ul TCL buffer (Qiagen) containing 1% 2-mercaptoethanol (Sigma-Aldrich). For cultured cells in 96-well plate, 20ul TCL buffer containing 1% 2-mercaptoethanol was used to lyse cells and 5ul of that was submitted to RNA-seq. Floating adipocytes were lysed in QIAzol followed by RNA isolation according to the manufacturer's protocol and 2 ng RNA were resuspended in 5 microliters of TCL buffer containing 1% 2-mercaptoethanol. Library construction and sequencing and data processing were performed according to the standard Immunological Genome Project protocols (https://www.immgen.org/img/Protocols/ImmGenULI_RNAseq_methods.pdf).