GSM5931663: CalInfA1; Homo sapiens; OTHER
Genomic DNA in control and survival cells were both extracted with QIAamp DNA Blood Maxi Kit (QIAGEN, catalog no. 51192) and the sgRNA sequences were amplified via PCR. Illumina adaptors and barcodes were incorporated into the PCR primers. PCR amplified libraries were generated with independent barcodes to allow multiplexing.