GSM5662736: Leaves, Se_12h1; Medicago sativa; RNA-Seq
Total RNA was isolated and purified from plant leaf tissue at three times (0, 12, and 48 h) under 100 mg kg-1 Se (IV) treatment using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer's procedure. Approximately 10 μ g of total RNA representing a specific adipose type was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). Following purification, using divalent cations, the poly (A) - or poly (A) + RNA fractions were fragmented into small pieces under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to the final cDNA library according to the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp ( ± 50 bp). And then we performed the paired-end sequencing on an Illumina at the (lc-bio, China) following of the merchant's recommended protocol. The paired-end libraries was 300 bp ( ± 50 bp)