GSM5819023: withDox noOHT replicate 3; Homo sapiens; RNA-Seq
For RNA-seq, cells were harvested according to the protocol of the Quiagen RNAeasy Mini Kit. Total RNA was isolated by using the RNeasy Mini Kit (Qiagen). 1 µg of total RNA was used to isolate mRNA by using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). For RNA-seq, cDNA libraries were generated with purified mRNA by using NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs). The quality of the cDNA library was determined with the Fragment Analyzer (Advanced Analytical). Libraries were sequenced on the NextSeq500 platform (Illumina).