GSM5509194: In vitro 28.2-Y.pestis; Yersinia pestis; RNA-Seq
One, 24 and 48 hours post infection, mice were sacrificed, lungs were removed and used for RNA extraction. RNA was also extracted from bacteria grown in culture (at 28° and 37° C) and naive mice, as control. Total RNA was extracted using the RNeasy kit (Qiagen) and residual DNA was digested using RNase-free DNase (Qiagen). In order to enrich the mRNA bacteria grown in culture (In vitro) we used riboZiro rRNA removal kit for depletion of rRNA, in each sample and ribozero gold rRNA depletion for dual RNA samples (mouse and Y. pestis). Library construction using Illumina TruSeq chemistry