GSM6369035: Flox/Flox Untreated 5; Mus musculus; RNA-Seq
Cells were cultured for at least 3-4 days after seeding to ensure desynchronisation of circadian oscillators; medium was removed and cells were directly lysed on the plate with Trizol reagent, followed by RNA extraction according to standard protocols. RNA-seq libraries were prepared from triplicates/condition according to standard protocols (TruSeq® Stranded Total RNA Library Prep Gold, Illumina) using 1 µg starting material.