GSM6235171: S9CC47_R6_0; Homo sapiens; ATAC-seq
ATAC-seq was performed as described previously (Corces et al., 2017). Briefly, CNCCs were incubated with BCh media containing 200U/mL DNaseI (Worthington, LS002007) for 30 min AND harvested using accutase. Viable cells were counted using Countess Automated Cell Counter (Invitrogen), 50,000 viable cells were pelleted at 500 RCF for 5 min at 4°C and resuspended in ATAC-resuspension buffer containing 0.1% NP40, 0.1% Tween20, and 0.01% Digitonin and incubated on ice for 3 minutes. Following wash-out with cold ATAC-Resuspension Buffer (RSB, 10 mM Tris-HCl pH 7.4, 10mM NaCl, 3mM MgCl2 in sterile water) containing 0.1% Tween20, cells were pelleted and resuspended in 50 μ