GSM5818959: Pete_Sp_PC_GEX; Mus musculus; RNA-Seq
ASCs from the bone marrow, spleen and thymus of 3 months old female and male Prdm1-eYFP mice were sorted on a BD Melody instrument. Each sort consisted of a pool of 3 mice and ASCs were gated as B220+/- CD138HI IgD- CD90.2- Prdm1-eYFP+. Sorted ASCs from each sex and organ were processed in individual GEM wells as separate samples on a 10x Genomics Chromium Controller. 5' Gene Expression (GEX) Libraries and VDJ Libraries were constructed following the manufacturer's guidelines using the Chromium Next GEM Single Cell 5' Library and Gel Bead Kit v1.1 (Catolog # 1000165) and Chromium Single Cell V(D)J Enrichment Kit, Mouse B Cell (Catolog # 1000072). VDJ Libraries were sequenced on an Illumina MiSeq. GEX Libraries were sequenced on a NovaSeq.