GSM5695914: BC_Pt7; Homo sapiens; RNA-Seq
Tumor genomic DNA and RNA were extracted from frozen tumor tissues using an AllPrep DNA/RNA Mini Kit (Qiagen). Peripheral blood mononuclear cells (PBMC) were isolated from the freshly withdrawn blood samples, and subsequently, the germline DNA was extracted using the QIAamp DNA Mini Kit (Qiagen) kit. The RNA seq library from tumor RNA was prepared using Illumina TruSeq Stranded mRNA Library Prep Kit as per the manufacturer's instruction.