GSM5221003: antigen-specific CD8+ T cells Trp2 (Dct) knockout single cells_day3; Mus musculus; RNA-Seq
Bulk RNAseq (samples 1-6): cells were isolated from three separate cohorts, with each cohort comprising eight WT and eight Dct-/- mice; Qiagen RNEasy Plus Mini Kit was used to extract RNA. Single cell RNA-seq (scRNAseq samples 1 and 2): sorted cells were supplied as input to the 10x Genomics single cell protocol. Bulk: Clontech StrandedRNA Pico Mammalian workflow was used for library preparation; scRNAseq: Chromium Single Cell Immune Profiling was used for library preparation. Samples were prepared for sequencing using standard Illumina protocols.