GSM5971453: GBM EC, 5377-sgPHGDH; Homo sapiens; RNA-Seq
2 x 10^6 cells were lysed in 1 ml TRIzol (Thermo Fisher), followed by RNA extraction. The isolated RNA was purified with an RNeasy Plus Mini Kit (Qiagen). The DNA library was constructed by using a RiboZero protocol with TapeStation (Agilent), and subjected to deep sequencing (125 PE, approximately 40 megabyte reads for each sample, Illumina HiSeq 2500) at the Children's Hospital of Philadelphia.