GSM5695942: No active effector inoculated cells Replicate1; Homo sapiens; RNA-Seq
Following 60 minute bacterial treatment, T84 cells were washed once with cold 1x PBS and mRNA was extracted using the Qiagen RNeasy Kit (Qiagen, #74014) following manufacturers instructions. The Illumina TruSeq Stranded mRNA Library Preparation Kit was used to prepare sequencing libraries from 750 ng of high-quality RNA samples (RIN=10). The Kit procedure was performed without modifications. This procedure includes mRNA purification and fragmentation, cDNA synthesis, 3' end adenylation, Illumina adapter ligation, library PCR amplification and validation. lllumina NextSeq 500 Sequencer was used to sequence the libraries with the production of single-end, 75 bp reads.