GSM6808308: ID β Hltf KO Index603_OtC7519; Mus musculus; RNA-Seq
Because pancreata are ribonuclease-rich, trunks of decapitated newborn mice were perfused in situ with RNAlater by insertion of a 20g-1-inch needle attached to a 5 ml syringe into the abdomen via the crural (posterior) attachment of the diaphragm . RNA stabilization occurred concomitant with the initial stretching of the pancreas. Pancreata were stored in RNA later at -70C until total RNA was isolated. RNA integrity and purity were assessed (Agilent Bioanalyzer) for 10 samples, i.e. 3 from β Hltf KO IC mice with low (<15 mg/dL) blood sugar, 3 from Hltf +/+ controls, and 4 from β Hltf KO ID mice. cDNA was generated from Ribo-Zero Plus rRNA-depleted samples and subjected to Illumina library preparation. Libraries were sequenced utilizing Illumina sequencing technology.