GSM6588292: GRMut2; Danio rerio; RNA-Seq
Adult zebrafish brain were dissected (together with pituitary glands) and RNA was extracted via Qiagen RNeasy kit (dissolved in RNAse free water). Library preparation step was performed with the input of 500ng total RNA (quality was checked on a 1% TAE agarose gel and with Agilent Bioanalyzer), following Illumina Ultra II Directiona RNA Lib Prep protocol (using NEBNext Ultra II RNA Kit). cDNA Library construction procedure included: mRNA inrichment by poly-dT pull down (NEBNext Poly(A) Kit) and 9 PCR cycles during cDNA library preparation with NEB adaptors. mRNA libraries were sequenced using a 375-390bp paired-end method.