GSM5818613: NHBE-4_submerged: NHBE submerged rep 4; Homo sapiens; RNA-Seq
Total RNA was extracted using RLT buffer supplemented with Beta-mercaptoethanol (Qiagen) according to the manufacturer's instructions. Isolated RNA was quantified by spectrophotometry, and RNA concentrations were normalized. PolyA enriched RNA was sequenced on llumina HiSeq 2500 using TruSeq SBS Kit v4 reagents non stranded Total RNA was extracted using RLT buffer supplemented with Beta-mercaptoethanol (Qiagen) according to the manufacturer's instructions. Isolated RNA was quantified by spectrophotometry, and RNA concentrations were normalized.