GSM6998327: Vdrflox/flox_skin_P18_2; Mus musculus; RNA-Seq
P18 mice back skin from epidermis specific VDR knockout (VDR cKO)(Vdr flox/flox; Krt14cre) and control (Vdr flox/flox) mice were collected and placed dermis down in 0.25% trypsin for 20 min at 37°C. Cell suspensions were obtained by scraping the skin gently. The cells were then filtered with strainers (70,40 μm). Library was performed according to the manufacter's instructions (RNAdia 2.0 kit, Dolomite Bio, Royston, UK). Briefly, cells were loaded into a Nadia (Dolomite Bio) microfluidics cartridge at a concentration of 310 cells per microliter. Cells were lysed in droplet and emulsion formed from the microfluidics device was then isolated and droplets broken with 1H,1H,2H,2H-Perfluoro-1-octanol. Reverse transcription was then performed, and purified cDNA was used as an input for Nextera tagmentation reactions.