GSM5456218: Input DNA for L80A_Oct4_ChIPSeq_2; Mus musculus; ChIP-Seq
Cells were lysed in lysis buffer 1 (50 mM HEPES-KOH [pH 7.5], 140 mM NaCl, 1 mM 0.5 M EDTA, 10% glycerol, 0.5% IGEPAL CA630, 0.25% Triton X-100) containing protease inhibitor cocktail (Sigma) for 30 min at 4°C and washed in lysis buffer 2 (10 mM Tris-HCl [pH 8.0], 200 mM NaCl, 1 mM EDTA, 0.5 mM EGTA). Chromatin was sheared using Diagenode Bioruptor (high power, 40 cycles of 30 s on and 30 s off) equipped with a water-cooling system (4°C) in 1 mL of sonication buffer (50 mM Tris-HCl [pH 8.0], 10 mM EDTA, 0.5% SDS) per 1 ´ 107 cells. ChIP seq sequencing libraries were prepared according to the instruction of KAPA Hyper Prep Kit (Roche).