GSM7047710: HCT116 short-read RNA-seq; Homo sapiens; ncRNA-Seq
At 80% confluence, RNA was extracted using Qiagen RNeasy Mini kit (74104) following manufacturer's directions For short read RNA-seq, samples were sent to Novogene Co., Ltd (Beijing) for library preparation and illumina sequencing. Briefly, mRNA was enriched with oligo(dT) beads and then fragmented randomly. cDNA was synthesised from fragmented mRNA using random hexamers as primers and then subject to second-strand synthesis, end-pair, dA-tailing and ligation of sequencing adapters. Sequencing was performed on an Illumina Novaseq 6000 instrument.