GSM6617152: INPUT-DMSO_4hrs_TRE3G-WT-HA_5PhIAA_Dox_R3; Homo sapiens; ChIP-Seq
20million cells were fixed for 5mins with 1% formaldehyde.  Covaris truChIP chromatin shearing kit was used to prepare chromatin.  Spike-in control was included (Active Motif). Chromatin was incubated overnight with 10ug CTCF antibody (Diagenode). 50ul Protein G dynabeads were added and incubated for 4hrs at 4oC.  For HA tagged samples, spike in was not included.  Sheared chromatin was incubated for 4hrs with  25ul Anti-HA magnetic beads (Pierce).  For all samples, after washing DNA was decrosslinked and eluted in TE+1%SDS, Proteinase K, 400uM NaCl.  DNA was purified by phenol cholorform isoamyl alcohol.