GSM6235058: LV NK, rep2; Mus musculus; RNA-Seq
2,000 IL-7R(-) ILC1s [CD3(-)NK1.1(+)NKp46(+)CD49a(+)CD49b(lo)IL-7R(-)], IL-7R(+) ILC1s [CD3(-)NK1.1(+)NKp46(+)CD49a(+)CD49b(lo)IL-7R(+)], bone marrow immature ILC1s [CD3(-)NK1.1(+)NKp46(+)CD49a(+)CD49b(lo)], bone marrow and liver NK cells [CD3(-)NK1.1(+)NKp46(+)CD49a(-)CD49b(+)], fetal liver group 1 ILCs [CD3(-)NK1.1(+)NKp46(+)CD49a(int)CD49b(int)], small intestinal lamina propria ILC1s [CD3(-)NK1.1(+)NKp46(+)CD27(+)IL-7R(+)], and salivary gland group 1 ILCs [CD3(-)NK1.1(+)NKp46(+)CD49a(+)CD49b(+)] were sorted by BD FACSAria 3, and cells were lysed by Buffer RLT (QIAGEN). Then total RNA was harvested using Agencourt AMPure XP (Beckman Coulter) size selection beads. Takara SMART-seq HT kit (Takara Bio) was used with all extracted RNA for cDNA synthesis. Takara SMART-seq Library Prep kit (Takara Bio) was used with 1 ng of cDNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Takara HT Plus kit protocols