GSM6765127: AM6_03_Rep_2_control; Homo sapiens; RNA-Seq
RNA was extracted from cells using the RNeasy Kit (Qiagen) following the instructions of the manufacturer. Indexed PolyA libraries were prepared using 200ng of total RNA and 14 cycles of amplification with the Agilent SureSelect Strand Specific RNA Library Prep Kit for Illumina Sequencing (Agilent, Cat No: G9691B). Libraries were quantified by qPCR using a Kapa Library Quantification Kit for Illumina sequencing platforms (Roche, Cat No: 07960336001). Single read 75bp sequencing was carried out by clustering 2.0pM of the pooled libraries on a NextSeq 500 sequencer (Illumina Inc.).