GSM5907388: POW3; Mus musculus; Bisulfite-Seq
DNA was isolated from the soleus myonuclei using the Qiagen DNA Micro Kit, using carrier RNA, according to the manufacturer protocol. “Classic” RRBS library preparation was performed by digesting 10 ng of genomic DNA with 30 units of MspI enzyme (New England BioLabs), and fragments were ligated to pre-annealed adapters containing 5'-methyl-cyotosine. Adapter-ligated fragments ≥50 bp were recovered using the DNA Clean & Concentrator Kit (Zymo Research, D4003) and bisulfite-treated using the EZ DNA Methylation-Lightning Kit (Zymo Research, D5030). Preparative-scale PCR was performed, and the products were purified again using the Clean and Concentrator kit.