GSM6617854: 145790 Late MF; Homo sapiens; RNA-Seq
Cryopreserved BMMCs/PBMCs Non-lymphoid CD34 cells were identified by the following immunostaining panel: CD34, CD38, CD3, CD7, CD19, and 7AAD. 7AAD- CD3-CD7- CD19- CD34+ cells were sorted and subsequently labeled with a panel of oligonucleotide conjugated antibodies according to TotalSeqA protocol from 10X Genomics (Pleasanton, CA). Oligonucleotide labeled cells were subjected to droplet bead capture, followed by cell lysis, reverse transcription, and amplification following protocol.