GSM6808327: T21 V V rep2; Homo sapiens; RNA-Seq
4 hours after vehicle or IFNγ treatment, cells were collected, spun down, washed once in PBS, then resuspended in TRIzol reagent (Invitrogen, 15596026) and snap frozen. After all 3 biological replicates were collected, cell lysates in TRIzol were thawed and total RNA was extracted according to the manufacturer's protocol. RNA concentration was measured by Qubit and quality was verified using a TapeStation Protocol for Universal Plus™ mRNA-Seq library preparation kit with NuQuant® (Tecan, 0520) was used Universal Plus™ mRNA-Seq library preparation kit with NuQuant® (Tecan, 0520)