GSM6891973: DDT_6; Mus musculus; RNA-Seq
Total RNA was isolated from snap frozen mouse tissues (mammary tumors and embryos at the blastocyst stage) using Qiagen's RNeasy extraction kit, according to the manufacturer's instructions. RNA purity, integrity and quality was examined by Bioanalyzer 2100 (Agilent Technologies) and Qubit (Thermo Fisher Scientific) Transcript libraries were constructed using the TruSeq mRNA Library Prep Kit (Illumina)