GSM5509245: mNET_seq_DMSO4h_Trip_0min_rep1; Mus musculus; OTHER
Cells were seeded the day before the experiment to get 100 million cells per sample the following day. We randomly assigned flasks for each treatment and treated cells with DMSO only or Auxin ligand, before extracting the chromatin-bound RNA Pol II. The cells were first washed with ice-cold DPBS, resuspended in 4 mL ice-cold HLB+N buffer (10 mM Tris-HCl (pH 7.5), 10 mM NaCl, 2.5 mM MgCl2, 0.5% (vol/vol) NP-40 and 1× proteinase inhibitor) and incubated on ice for 5 minutes, then cells were scrapped to a 15-mL centrifugate tube. Libraries were constructed using NEBNext Multiplex Small RNA Library Prep kit (NEB, NC0477293).