GSM5695771: EndoMT4; Homo sapiens; RNA-Seq
At day 14, cells cultures on 4 kPa hydrogel and TC with and without TGF-β stimulation were dissociated and resuspended in DMEM supplemented with 10% FBS. Single cell libraries were prepared using a 10X Chromium v3 microfluidic chip, targeting for 8000 cells per sample. The barcoded libraries from individual samples were then pooled and multiplexed, followed by sequencing on an Illumina NovaSeq 6000 platform using a NovaSeq-S1 (2x100bp) flow cell.