GSM5931672: Wild-type Male + Vehicle_pool2; Mus musculus; RNA-Seq
RNeasy Plus Mini Kit (Qiagen, Valencia, CA, USA) was used to extract total RNA from individual livers, followed by quality assessment of RNA by TapeStation 4200 (Agilent, Santa Clara, CA, USA), to identify high quality RNA samples (RIN>9.0) for pooling, with each pool comprised of RNA obtained from n=3-5 individual livers. Stranded RNA-seq libraries were prepared from polyA-selected total liver RNA using an Illumina stranded TruSeq mRNA Prep Kit (Illumina, San Diego, CA, USA). Three independent RNA pools were prepared for each of the 4 treatment groups, with each pool comprised of n = 3-5 individual liver RNA samples. RNA-seq libraries were subjected to 150 cycle paired-end sequencing using an Illumina HiSeq 2500 instrument (Illumina) for each of the 12 RNA-seq libraries.