GSM6617904: LALB-HIC-Pro-Cap; Homo sapiens; Hi-C
Promoter-Capture HiC : Cells were crosslinked with 1% formaldheyde, digested with a restriction enzyme cocktail, end-labeled with Biotin-14-dATP and then followed by ligation. The ligated chromatin was reverse cross-linked and sonicated using Bioruptor ultrasonicator to produce 300-500 bp fragments. Fragmented DNA was then size-selected to have a size distribution between 200-600 bp, and finally subjected to biotin enrichment Promoter-Caputre HiC : DNA libraries were prepared using Accel-NGS 2S Plus DNA Library Kit (Swift Bioscences, Cat. No. 21024), and the resulting libraries were amplified using the KAPA library amplification kit. Subsequently libraries were hybridized to specific SureSelect XT Human capture libraries (Agilent Technologies) and sequenced in paired-end mode (2×75 bp) with NextSeq 500 (Illumina, CA).