GSM6765068: To H3K4me1, developing ovary part rep 2; Talpa occidentalis; ChIP-Seq
Gonads were fixed using 1% formaldehyde and subsequently snap-frozen and stored at -80°C. Chromatin immunoprecipitations were performed using the iDeal ChIP-seq Kit for Histones (Diagenode, Cat. No. C01010051) according to the manufacturer's instructions. Whole fixed gonads were lysed and subsequently sonicated using a Bioruptor (45 cycles, 30 seconds on, 30 seconds off, at high power) in the provided buffers. 5 µg of sheared chromatin were then used per immunoprecipitation with 1 µg of the following specific histone antibodies: anti-H3K4me3 (Millipore, cat. No. 07-473), anti-H3K4me1 (Diagenode, cat. No. C15410037), anti-H3K27ac (Diagenode, cat. No. C15410174)