GSM6543017: Human meningoma, QM171; Homo sapiens; RNA-Seq
Frozen meningiomas were mechanically lysed using a TissueLyser II (QIAGEN) according to the manufacturer's instructions. RNA were extracted from lysed tissue using the AllPrep DNA/RNA/miRNA Universal Kit (#80224, QIAGEN). RNA quality were initially assessed using a NanoDrop One (Thermo Fisher Scientific). RNA samples with 260/280 values less than 1.8 or 260/230 values less than 1.6 were cleaned using the RNA Cleanup protocol from the RNeasy Mini Kit (#74106, QIAGEN). library preparation was performed using the TruSeq Standard mRNA Kit (#20020595, Illumina)