GSM6724026: W0, replicate 1, scRNAseq; Mus musculus; RNA-Seq
Total RNA was extracted using Rnaeasy Kit on QIAcute (Qiagen) from resting and stimualted sorted naive CD8+ T cells (24 hours with anti-CD3/28 antibodies) from Kmt2d WT or Kmt2d KO mice. RNA samples were processed using the Illumina TruSeq Stranded mRNA Sample Preparation Kit. Poly (A) tailed RNA was purified from 1 ug of total RNA, fragmented, and reversed-transcribed into cDNAs. Double strand cDNAs were adenylated at the 3' ends and invidually indexed, followed by limited-cycle amplication. Library was performed according to the manufacter's instructions (single cell 5' R2-only protocol, 10x Genomics).