GSM6543806: Y21SS00263; Homo sapiens; Mus musculus; RNA-Seq
Human peripheral blood mononuclear cells (PBMCs) were transferred into immunodeficient mice to generate a mouse model with humanized T cells. Ischemic hemispheres of humanized mice were perfused with ice-cold saline and freshly harvested at 0 or 3 days after tMCAO. Single cell suspensions were prepared for flow cytometry. Single cells were incubated with FITC anti-human CD45 and APC/Cyanine7 anti-mouse CD45 for 30 minutes on ice at 4 ℃ in the dark before stained with 7-AAD for 5 minutes. The live CD45high cells (human and mouse) were sorted by FACS Aria SORP (BD Bioscience). At last, we mix human and mouse CD45high cells with equal proportion Library was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics).