GSM6998557: Tfamflox/flox Cre-lyz2 +TS rep 1; Mus musculus; RNA-Seq
Total RNA was isolated from BMDCs (treated with or without TS ) of Tfam-/- and control mice using the TRIzol reagent kit. RNA integrity was assessed using the RNA Nano 6000 Assay Kit by the Bioanalyzer 2100 system (Agilent Technologies, CA, USA), and samples with RIN scores above 6 and a minimum total RNA of 100 ng were used for further library construction. PCR products were purified using the AMPure XP system, and library quality was assessed using the Agilent Bioanalyzer 2100 system.