GSM6808146: DES7S_total mRNA_rep3; Saccharomyces cerevisiae; OTHER
Total RNA extracted by hot acid penol extraction from lysate and mRNA was enriched using OligodT-beads. mRNA was randomly fragmented and ligated to deepSeq adapters. mRNA fragments ranging rom 24-28nt were size excluded and dephosphorylated. Adapters were ligated and the resulting fragments were circularized. cDNA was prepared with circRNA as input and PCR amplicification was performed for 4-8 cycles.