GSM6235128: Capture C DMSO_1; Homo sapiens; OTHER
Cells were fixed with 2% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was digested by DpnII and religated. Decrosslinking and DNA extraction was performed. Samples were sonicated using a S220 focused-ultrasonicator (Covaris). Capture-C libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina. Libraries were amplified using 6 cycles on the thermocycler. Capture enrichment was done with IDT biotinylated DNA oligonucleotides and washes were performed using Roche KAPA HyperCapture Reagent Kit Post amplification libraries were purified using Agencourt AMPure XP beads from Beckman Coulter. Libraries were validated using the Agilent High Sensitivity NGS Analysis Kit.