GSM6588957: Donor Control 1; Homo sapiens; RNA-Seq
Cells were harvested from culture using accutase, stained with CD31 and VE-cadherin antibodies, labeled with Dapi, and sorted. Sorted cells were processed for RNA isolation using a Qiagen Mini Kit and RNA was sequenced. RNA-seq libraries of polyadenylated RNA were prepared using the TruSeq RNA Library Prep Kit v2 (Illumina) or TruSeq Stranded mRNA Library Prep Kit (Illumina) according to the manufacturer's instructions.