GSM7086175: DG1030; Homo sapiens; RNA-Seq
Total RNA was extracted from frozen tissue sections (10-40 10um sections depending on tissue face size) using Qiagen RNA blood and tissue kits following manufacturers protocols cDNA libraries for 28 CCOC and 29 ENOC were prepared using strand‐specific RNA‐Seq Sample Preparation kit (stranded, polyA+) from Illumina. Data was generated from paired‐end sequencing at Canada's Michael Smith Genome Sciences Centre on Illumina platforms (San Diego, CA, USA): HiSeq 2500 using V3 or V4 chemistry and paired‐end 125 base reads targeting 200 million paired‐end reads per sample.