GSM6617896: Healthy_3_RNAseq; Homo sapiens; RNA-Seq
Total RNA was extracted from patient samples using Qiazol (Qiagen, IT), purified from DNA contamination through a DNase I (Qiagen, IT) digestion step and further enriched by Qiagen RNeasy columns for gene expression profiling (Qiagen, IT) RNA libraries were generated using the same amount of RNA for each sample according to the Illumina TruSeq Stranded Total RNA kit with an initial ribosomal depletion step using Ribo Zero Gold (Illumina, CA). The libraries were quantified by qPCR and sequenced in paired-end mode (2×75 bp) with NextSeq 500 (Illumina, CA).