GSM7047695: 44_MIBC_MACE; Homo sapiens; RNA-Seq
A 1mm punch was taken from FFPE blocks of a representative tumor area with at least 50% tumor content. RNA was isolated using the “truXTRAC FFPE total NA Kit – Column” (Covaris, Woburn, MA, USA) The samples were prepared by GenXPro GmbH (Frankfurt, Germany) using the MACE-Kit V2 according to the manual of the manufacturers (GenXPro GmbH). Briefly, RNA was fragmented and polyadenylated mRNA was enriched by poly-A specific reverse transcription and, a specific adapter was integrated at the 5'ends and the products were amplified by competitive PCR and sequenced on an Illumina NextSeq 550 instrument.