GSM5391570: smrna_hela_shcontrol_cytoplasm_r1; Homo sapiens; ncRNA-Seq
Cells were fractionated into nucleus and cytoplasm according to Bhatt et al., Cell 2012, https://doi.org/10.1016/j.cell.2012.05.043. Nuclear RNA was extracted using Trizol reagent  (Thermo Fisher Scientific, #15596026), cytoplasmic RNA using Trizol LS reagent (Thermo Fisher Scientific #10296028) according to the manufacturer's instructions. Genomic DNA was removed by Turbo DNAse treatment Small RNA libraries were prepared using the SMARTer smRNA-seq kit (Takara, #635030) with 700 ng of total RNA; 5% (35 ng) of Drosophila RNA were added as spike-in