GSM5695928: Ythdf2 cKO; Mus musculus; RNA-Seq
The cell suspension (300-600 living cells per microliter) was loaded onto the Chromium single cell controller (10 x Genomics) to generate single-cell gel beads in the emulsion according to the manufacturer's protocol. Libraries were subsequently prepared sing Single Cell 5'Library and Gel Bead Kit and sequenced on an Illumina NovaSeq 6000 instrument with pair-end 100 bp (PE100) reading strategy.