GSM5509447: ChIP_Seq_Pol_I_IAA_anti_Pol_II_rep2; Mus musculus; ChIP-Seq
Cross-linked cells were subjected to chromatin fraction isolation as reported (Ji et al., 2015), and the chromatin was pretreated with 40U MNase (NEB, M0247), followed by sonication as previously described (Jiang et al., 2020). 1μg antibodies and 30μl Protein G beads (Thermo Fisher, 10004D) were used for each replicate.. ChIP DNA were subjected to TELP library construction (Peng et al., 2015). 200bp-400bp DNA libraries were gel-selected, purified, and delivered to Novogene company for sequencing.