GSM5390830: sgKmt2c - Rep1; Mus musculus; RNA-Seq
Tumors were dissected and incubated with 10% collagenase in DMEM/F12 for 45 minutes followed by 10 minutes of digestion in trypsin-EDTA. GFP+,DAPI- cells sorted by flow cytometry prior to RNA extraction by Zymo RNA mini-prep kit RNA libraries were prepared for sequencing using standard Illumina protocols