GSM6048796: 78 Hippocampus, 22M; Mus musculus; RNA-Seq
The total RNA was extracted from young (3M) and aging (22M) hippocampus samples in mice by using Trizol reagents (Invitrogen). The extracted total RNA samples were subjected to agarose electrophoresis and Nanodrop for quality inspection and quantification.1-2g total RNA was selected from each sample for sequencing library construction. Oligo magnetic beads were used to enrich the mRNA, and the KAPA Stranded RNA-Seq Library Prep Kit (Illumina) was used to construct the library