GSM6543183: Control_22mo_4; Mus musculus; RNA-Seq
Tibialis anterior muscles were collected, flash frozen on liquid nitrogen, and RNA was harvested using Trizol reagent (Invitrogen) and purified using the RNeasy kit and RNase-free DNase Set (Qiagen). Samples were quantified using the Trinean DropSense 16 and RNA quality was assessed using the Agilent BioAnalyzer, with all samples showing an RNA integrity Number (RIN) greater than 8. Libraries were prepared using the Illumina TruSeq Stranded mRNA Sample Prep Kit.