GSM6588991: InputLacZOsmostress_rep1; Drosophila melanogaster; ChIP-Seq
After respective treatments, cells were harvested and processed according to experiment. Hi-C and ChIP-seq cells were crosslinked with 1% formaldehyde for 10 min followed by 5 min glycine treatment to quench crosslinking reaction, flash freezed and stored at -80°C. Cells were washed with Buffer A (10 mM Hepes pH 7.9, 10 mM EDTA, 0.5 mM EGTA, 0.25% Triton X100) and Buffer B (10 mM Hepes pH 7.9, 100 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 0.01% Triton X100) for 10 min each. These cells were resuspended in sonication buffer (1X TE, 1X SDS, 1mMPMSF). chromatin was sheared with a Bioruptor sonication device (Diagenode) to an average fragment size of 300-500 bp (25 cycles; 30 sec on / 30 sec off). Sheared chromatin was pulled down by respective antibodies and used for library preparation. These libraries were sequenced and analyzed.