GSM6310712: HCT116 treated with PLOD3 siRNA 3; Homo sapiens; RNA-Seq
Total RNA was isolated using RNAiso Plus (Takara, Shiga, Japan), and 1 µg RNA was used to synthesize cDNA with the PrimeScript™RT reagent Kit with gDNA Eraser (Takara). Libraries were constructed by depleting ribosomal RNA using the Human/Mouse/Rat Ribo-Zero rRNA Removal Kit for Illumina (Epicentre, Madison, WI, USA).