GSM6235389: HUVECs_NC_Sample 3; Homo sapiens; RNA-Seq
The total RNA from primary cells was extracted using Trizol (Invitrogen) following the manufacturer's procedure. The quality and integrity of each sample were checked using the Agilent BioAnalyzer 2100 (Agilent RNA 6000 nano kit) and a NanoDrop ND-1000 spectrophotometer (NanoDrop Technology, Rockland, DE). 1 mg of RNA was used as input material for the RNA sample preparations. Paired-end libraries were synthesized by using the mRNA-seq Lib Prep Kit for Illumina (ABclonal, Wuhan, Hubei, China).