GSM6543583: high IL-2, bio rep 2; Mus musculus; RNA-Seq
Total RNA were extracted using RNeasy Plus Micro or Mini Kit (Qiagen). A total amount of 3 µg RNA per sample was used as input material for the RNA libraries preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.