GSM5391018: CD8+_T; Homo sapiens; RNA-Seq
scRNA-seq libraries were generated using the SureCell WTA 3′ Library Prep Kit for the ddSEQ System (20014280, Illumina) with the following modifications. A higher concentration of beads was used to obtain 1,000-2,000 single-cells per emulsion, whilst minimizing the number of droplets with multiple beads to < 10%. Furthermore, Bst 2.0 WarmStart (M0538S, NEB) was added to the droplet mix to perform temperature activated second strand synthesis in droplets.