GSM5388450: MFi2CLTSCs_tdTomato_negative_rep2; Mus musculus; RNA-Seq
Cells were digested as single cells and added into gelatin-coated plate for 30minutes to get rid of feeder cells, supernatants containing 2C-like totipotent stem cells were separately collected and spined as samples,  flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit V2 (Vazyme) was used  to prepare a llumina Truseq librariesw. RNA libraries were prepared for sequencing using the establblished protocols (Wu, et al., Nature, 2016; Dobin, et al., Bioinformatics, 2013)