GSM5971518: Mouse GBM tumor tissue 3; Mus musculus; RNA-Seq
Cdh5-CreERT2;Rosa-LSL-tdTomato mice bearing RCAS-induced tumors were euthanized and perfused with PBS supplemented with EDTA. GBM tumors and brain tissue from a healthy mouse were harvested and digested with collagenase-II and DNase. Single cell suspension was prepared after filtering using a mesh strainer with 100-μm pores. Cell samples were prepared and analyzed following a manufacturer's V3 library protocol (10x Genomics) and single-cell RNA-seq at the next-generation sequencing core at the University of Pennsylvania. scRNA-Seq