HiSeq X Ten paired end sequencing; Antibiotic-induced Alternations are Associated with the Suppression of Immune-related Pathways in Grass Carp
The fish were unsystematically divided into three groups of 270 fish with triplicates. The corresponding fish dietary treatments were prepared using a basic feed (purchased from Tongwei Group, Chengdu, China) for the control group (CG) and the same but supplemented with enrofloxacin (EG) or florfenicol (FG) at a concentration of 10 mg/kg.The midgut tissues of 12 individual fish from each of the three treatment groups were snap-frozen and aseptically harvested in liquid nitrogen for the transcriptomic analyses. Total RNA was extracted from the midgut of grass carp by using the TRlzol Reagent and following the manufacturerâ€™s instructions (Life Technologies, California, USA).  cDNA libraries were constructed as per the instruction manual of NEBNext Ultra RNA Library Prep Kit.