GSM6808148: DES7S_RiboSeq_rep2; Saccharomyces cerevisiae; OTHER
Lysates were digested with RNAse1 and 80S ribosomes extracted from a density gradient after Ultracentrifugation. Size excluded RNA fragments were ligated to DeepSeq adapters. mRNA fragments ranging rom 24-28nt were size excluded and dephosphorylated. Adapters were ligated and the resulting fragments were circularized. cDNA was prepared with circRNA as input and PCR amplicification was performed for 4-8 cycles.