GSM6543645: Unperturbed H3K4me1 biological replicate 2; Homo sapiens; ChIP-Seq
MEXF 2090 cells grown in either unperturbed conditions or under glutamine deprivation for 2 days were cross-linked using 1% formaldehyde for 10 minutes. The reaction was stopped by incubation for five minutes with 0.125M glycine. Cell pellets were washed twice with cold PBS containing protease inhibitor. Nuclear lysates were sonicated using a Bioruptor Pico for 6 cycles, 30s ON, 30s OFF settings. Libraries were made using NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs) kit (NEB, E6440S) following manufacturer's instructions and were sequenced on an Illumina NovaSeq platform.