Illumina HiSeq 2000 paired end sequencing; RNA-seq of human non-small-cell lung cancer cell line A549 treated with Feiyanning
A549 cells were treated with FYN, then cultured for 24 h.   Total RNA were extracted using TrizoI. mRNA were retain using Oligotex mRNA Mini kit. The  mRNA  were fragmented into small fragments and reverse transcripted into cDNA. Next, the cDNA fragments were purified with Ampure beads, end repaired, poly(A) added, and ligated to Illumina sequencing adapters. Then User enzyme was used to digest the second-strand cDNA. The products were amplified.