GSM5695816: Adrenal Dexamethasone rep2; Mus musculus; RNA-Seq
Adrenal glands were removed, and surrounding adipose tissue was trimmed away. The right adrenal gland from each mouse was snap-frozen in liquid nitrogen and stored at -80°C until use. Adrenals from 2-3 animals were pooled as one biological replicate, with two biological replicates per condition (a total of 5 mice were included in each condition). Total RNA was extracted from frozen adrenal glands using Monarch Total RNA Miniprep Kit. Sequencing libraries were generated using NEBNext® Ultra TM RNA Library Pre Kit for Illumina