GSM5818737: ChIP_L267_Input; Ovis aries; ChIP-Seq
Cells were cross-linked for 10 min at room temperature with 1% formaldehyde before lysis followed by sonication of their chromatin (Bioruptor Plus, Diagenode) to obtain DNA fragments of 200–400 bp. Chromatin immunoprecipitations were performed with chromatin from 6x10^6 cells (1x10^6 cells for the epigenetic modifications) and 5μg of antibodies Library was prepared using the Ovation Ultralow System v2 kit (NuGen) following Manufacter's instructions. Paired-end sequencing was then performed with the Illumina HiSeq 2000 instrument. More than 20 millions of single reads were obtained for all libraries