GSM5389950: Shoot_rep2; Cuscuta campestris; RNA-Seq
Samples were collected 15 days post inoculation. Plants with healthy holdfasts and new developed shoots were detached from the artificial host. For haustorial samples, regions harboring visibly healthy holdfasts (identified by formation of new shoots) were separated from any shoot or lateral meristem. For non-haustorial stem samples, new healthy and turgid shoots were collected after removing 5 mm of the stem connecting to the haustorial region to avoid tissue contamination. All tissues were immediately frozen in liquid nitrogen. Samples from 3-4 plants were pooled in each replicate and four replicates were used for each type of tissue. For RNA extraction, frozen tissue was ground using glass beads in a bead beater and RNeasy Plant Mini Kit (Qiagen). After kit extraction, RNA was precipitated using 3M NaOAc and Ethanol 100%, and resuspended in RNAse-free water. mRNA library was prepared using Poly-A enrichment