GSM5390873: Pik3caHR; Mus musculus; ATAC-seq
Two mammary glands were disected and pooled per mouse. Glands were digested in 2x gentle collagenase/hyaluronidase for 2 hours, then treated with ammonium chloride and triturated for 2 minutes in pre-warmed trypsin followed by dispase. Cells were then sorted for GFP+ infected cells and immediately processed Libraries were constructed according to 10x Genomics protocol snATAC-sec