GSM7047569: ss_Cold_3h_Rep4; Manihot esculenta; RNA-Seq
Total RNA was isolated using the RNeasy Plant Mini Kit (Qiagen) and treated with Turbo DNase (ThermoFisher Scientific) according to manufacturers' instructions. DNase-treated RNA was recovered by acid–phenol extraction and ethanol precipitation. Strand specific RNA-Seq libraries were prepared using TruSeq stranded total RNA preparation kit from Illumina following manufacturer's protocol.