GSM5952363: normal adjacent tissues_100; Homo sapiens; RNA-Seq
RNA was extracted and the purity, concentration and integrity of RNA samples were detected by advanced equipment to ensure that qualified samples can be used for transcriptome sequencing. 1) primer annealing, reverse transcription to get cDNA, add switch oligo; 2) synthesis of complementary chains; 3) DNA damage repair and end repair, magnetic beads purification.