GSM5931665: Chow diet_rep 1; Mus musculus; RNA-Seq
Livers were flash frozen in liquid nitrogen, homogenized in Trizol (Invitrogen), and total RNA was isolated with the RNeasy mini kit (Qiagen). 1 µg of total RNA was used to isolate polyA RNA with the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England BioLabs, Ipswich, MA) and polyA RNA was enriched with the SMARTer Apollo NGS library prep system (Takara Bio USA, Mountain View, CA). The library was prepared with the dUTP-based stranded library NEBNext Ultra II Directional RNA Library Prep Kit (New England BioLabs).