GSM7047719: lung_SARS-CoV2pos_3dpi; Mesocricetus auratus; RNA-Seq
RNA was extracted from homogenized samples using Direct-zol RNA Miniprep Kit (Zymo research, Freiburg, Germany) following manufacturer's instructions. Extracted RNAs from 3 animals per group were pooled prior to library construction. Polyadenylated RNAs were isolated with the NEBNext Poly(A) mRNA module (New England Biolabs) and a modified protocol of the NNSR priming method was used to produce cDNA libraries. Sequencing was performed on an Illumina NextSeq550 instrument with a 86 base, single-end setting, using a custom sequencing program.