GSM6265663: pNSCs2; Mus musculus; RNA-Seq
Primary neurospheres were dissociated and carried on ice to the Drop-seq facility at Princess Margaret Genomics Centre for single cell capture, cDNA amplification, and sequencing library preparation (Macosko et al., 2015). Libraries were sequenced on an Illumina NextSeq500 to a depth of 60,000 reads per cell.