GSM5907746: Chd2_1_GFP; Mus musculus; RNA-Seq
Harvested spleens were mechanically dissociated to form a single cell suspension. Erythrocyte lysis was carried out using ACK buffer and B cells from edited and unedited fractions isolated by cell sorting. Cells were washed in cold PBS and total RNA was extracted using Qiagen RNAeasy kit. RNA-seq libraries were prepared using the TruSeq RNA Sample Prep Kit v2 (Illumina).