GSM5455435: M3 CD45-negative cells (QPCTL-WT) tumor bearing; Mus musculus; RNA-Seq
RNA was extracted from the indicated frozen tissues using the RNeasy Mini Kit (Qiagen). Flow-sorted cell populations were washed in PBS once, and subsequently lysed in RLT buffer (Qiagen). Whole transcriptome sequencing samples were prepared with the TruSeq Stranded mRNA Kit (Illumina). Paired-end 50 bp sequencing was performed on a NovaSeq 6000 system (S1 flowcell, Illumina), obtaining an average of 18x106 reads per sample. Whole transcriptome sequencing samples were prepared with the TruSeq Stranded mRNA Kit (Illumina).