GSM6311233: naïve CD4+ T cells, resting unstimulated, rep1; Homo sapiens; Hi-C
T cells were FACS sorted from human peripheral blood samples of three healthy donors. Naive T cells were defined as CD4+CD127+CD25-CD45RA+; memory Th2 cells as CD4+CD127+CD25-CD45RA-CCR6-CXCR3/5-CCR4+ Cells were collected and crosslinked with 1% formaldehyde. Nuclei were digested with MboI followed by fill-in with bio-dATP, in-situ ligation and DNA extraction. DNA was sonicated and libraries were constructed using the the NEBNext DNA Library Prep Kit (New England Biolabs). Libraries were amplified for 8-12 cycles using Illumina TruSeq primers. Amplified libraries were purified and size-selected (>250bp) using Ampure XP beads.