GSM6589278: Δcre-1-Glu_1; Thermothelomyces thermophilus ATCC 42464; RNA-Seq
Mycelia were harvested by vacuum filtration with Whatman filter paper, frozen immediately in liquid nitrogen. Total RNA from frozen samples was isolated using TRIzol reagent and an additional clean-up was performed for DNA degradation using the RNeasy mini kit (Qiagen). RNA libraries were prepared for sequencing using standard Illumina protocols.