GSM6204361: Trp/Trp kidney, biol rep 3 [TT_3]; Mus musculus; RNA-Seq
Kidneys isolated from mice after 4 weeks of Ang II infusion were stored in RNALater and homogenized in Trizol (Invitrogen) reagent using a rotor homogenizer. Separate kidneys underwent Percoll gradient ultracentrifugation followed by isolation of F4/80+ cells using anti-F4/80 microbeads (Miltenyi). Cell pellets were lysed and stored in Trizol. RNA isolation was performed using chloroform and isopropyl alcohol according to the Trizol manufacturer's instructions. cDNA library preparation was performed using a polyA-selected library preparation kit (New England Biolabs).