sRNA-Seq of Saccharomyces cerevisiae: +DCL4 +SSC
Total RNA was prepared by the hot-phenol method and resuspended in RNase-free water, and 2 micrograms of RNAs were used for sRNA and transcriptome analyses. Libraries of sRNAs were produced using a small RNA seq Kit (Bioo Scientific) and sequenced with standard sequencing oligos on the Illumina MiSeq platform.