Metagenomic sequencing of bat: oropharyngeal swab
RNA extraction was carried out using the QIAamp Viral RNA mini kit (Qiagen, Germany) according to the manufacturer's protocol. Single-strand cDNA synthesis was performed with reverse transriptase M-MuLVRH (Biolabmix, Russia), and double-strand cDNA synthesis was carried out using Klenow fragment (SibEnzyme, Russia). Library preparation was performed using NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, England) with fragmentation of cDNA to 2x150 bp fragments. Sequencing was performed with Illumina NovaSeq 6000 (Illumina, USA) with generation from 800 million paired reads per sample.