Targeted TCR sequencing of Homo sapiens: Adult female Small.bowel
In this study, 39 patients with ovarian and endometrial cancer underwent surgery and had tissue and blood samples collected. DNA was extracted from the samples and amplified using three steps of multiplex PCR. The amplified DNA was then sequenced using Illumina NovaSeq 6000 System and CDR3 sequences were called using MiXCR, with non-human sequences filtered out and clones with improper conserved residues excluded.