Stapled phage-displayed library screening with D-CHIP at 1000 nM (replicate 2)
Phage particles are denatured from magnetic beads with an added spike-in sequence (not a library member that is used to enable cross-well normalization of sequence reads), followed by a two-step low-cycled PCR to introduce Illumina adaptors and 10bp TruSeq DNA UD Indexes according to an Illuminas 16S Metagenomic Sequencing Library Preparation protocol. The NGS library is sequenced by an Illumina NovaSeq platform using a 2x150 bp high-output kit.