Targeted next generation sequencing of circular tumour DNA obtained from Neuroblastoma patients
Library source is circulating tumour DNA. DNA sequencing libraries were prepared using the QIAseq Targeted Custom DNA panel. 40ng of input DNA was used for each sample library. Fragmentation was performed by enzyme reaction followed by end repair and poly-A tail addition. Sequencing sample indexes and UMI indices were ligated to the ends of the DNA fragments.