WES of white cell: adult male
Genomic DNA was isolated from peripheral blood leukocytes using a QIAamp DNA Mini Kit (Qiagen, Hilden, Germany). All genomic DNA samples were of high quality with an optical density 260/280 ratio ranging from 1.8 to 2.0. Of the 381 subjects, 344 (61 cases of SCAD and 283 control patients) were analyzed using an Illumina HiSeq X sequencer or Novaseq sequencer