ONT sequencing of genome of Thermomicrobium sp. 4228-Ro
DNA was isolated with the Monarch genomic DNA purification kit (New England Biolabs). Nanopore sequencing was performed on MiniIon with the SQK-LSK109 protocol (Oxford Nanopore) for genomic DNA by ligation. Totally 200 fmoles ofgenomic DNA were used for the Oxford Nanopore R9.4.1 flow cell.