Targeted nanopore sequencing of BOK circRNA-derived amplicons (DU 145 cell line)
Total RNA from DU 145 cell line was reversely transcribed using random hexamers. Next, multiple nested PCR assays using divergent primers, specific for each BOK exon, were performed. After library construction, 3rd generation (nanopore-based) sequencing was conducted using the MinION Mk1C platform and the Flongle adapter.