Hi-C of Ornithorhynchus anatinus: male adult fibroblast replicate 1
To construct libraries, adaptors were ligated to DNA fragments using the NEBNext Multiplex Oligos for Illumina kit. Indexes were added via PCR amplification (38 cycles) using the NEBNext Ultra II Q5 Master Mix. PCR purification and fragment size selection were done using Agencourt AMPure XP beads. Paired-end reads were generated on the Illumina NovaSeq 6000 platform.