RNA-Seq of hom chondrocyte: patient 2
Ribosomal RNA was heavily removed from total RNA, resulting in mRNA. fragmentation buffer was added to break the RNA into short fragments, and the short fragments were used as templates to synthesize first-strand cDNA using a six-base random hexamers. Buffer, dNTPs (dUTP, dATP, dGTP, dCTP), and DNA polymerase I were then added to synthesize the double-stranded cDNA, which was subsequently purified using AMPure XP beads. The purified double-stranded cDNA was subjected to end repair, A-tail addition, sequencing adaptor ligation, fragment size selection with AMPure XP beads, and PCR to enrich the final cDNA library.