shotgun metagenomics analysis of oil-contaminated sandy beaches
Column microcosms of coastal seawater-brine mixture and sand amended with 30g/L of salt after 30 days of incubation. DNA was extracted using the Quick-DNA Fecal/Soil Microbe MidiPrep kit from Zymo Research and the librairies were prepared using the Nextera XT DNA Library Preparation Kit from Illumina. Products were pooled and sequenced on an Illumina NovaSeq on a 2 x 150 bp configuration.