Nanopore metagenome of methanogenic enrichment culture AM
The library was prepared with 60 ng genomic DNA using the ligation sequencing gDNA kit (ONT) following the manufacturers instructions and minor modifications. In short, DNA repair and end-prep were performed using the NEBNext FFPE DNA Repair Mix and NEBNext Ultra II End repair/dA-tailing Module (New England Biolabs), followed by the adapter ligation using Quick T4 ligase (New England Biolabs). Subsequently, a clean-up using AMPure beads (Beckman Coulter) and the short fragment buffer (SFB) was performed to retain DNA fragments of all sizes with a final incubation at 37 C for 10 min. The library was then directly loaded onto a primed SpotON R9.4.1 flow cell in a MinION Mk1C (1366 pores available). Sequencing was carried out for 44 h resulting in 1.57 M reads and 2.73 Gb raw data.