nanopore 93s rRNA sequencing of mouse: BALF sample
The cleaned DNA was repaired, end-repaired and A-tailed using NEBNext FFPE DNA Repair Mix (NEB, USA) and NEBNext Ultra II End Repair/Da-Tailing Module (NEB, USA). Then, the end repaired samples were ligated with native barcodes using the Native Barcoding Expansion 96 (EXP-NBD273; Oxford Nanopore Technologies, UK) and NEB Blunt/TA Ligase Master Mix (NEB, USA). Finally, the barcoded samples were pooled and ligated with adapter using Quick Ligation Kit (NEB, USA). All experiments were purified with AgencourtAMPure XP Beads (Beckman, USA).