RNA-Seq of leaves:Medicago sativa
Six-month-old plants were treated with 250 mM NaCl solution ( water culture system) for 2 hours. All of the plants were propagated by cutting and three biological replicates were set for each of the WT (WT1, WT2, and WT3) and OE-MsSPL12 lines (5,7 and 10). Libraries constructed from total RNA isolated from leaves were deep-sequenced on the NovaSeq6007 platform (Illumina).