ITS of Homo sapiens:adult male fecal sample
DNA of fecal samples from subjects was extracted by MagPure Soil DNA LQ Kit for Internal transcribed spacer I (ITS) sequencing. Concentration of DNA was verified via both NanoDrop and agarose gel. The genome DNA was used as template for PCR amplification with the barcoded primers and Tks Gflex DNA Polymerase.