RNA-seq of Mytilus galloprovincialis: 04 hpf R2
Unfertilized eggs, embryos and larvae were sampled with a 60 m mesh filter and concentrated in RNAse free 1.5 mL tubes. The tubes were then placed on ice and gently centrifuged multiple times to remove the seawater from the samples. The pellet was then immediately resuspended in lysis buffer, homogenized by energetic pipetting, and flash-frozen in liquid nitrogen. Lysed samples were stored at -80C until RNA extraction and purification that were carried out with the RNAqueous-Micro Kit (Invitrogen) and the RNeasy MinElute Cleanup Kit (Qiagen), respectively. Sequencing libraries were made by BGI following oligo-dT enrichment and pair-end sequenced using DNBSEQ-G400 platform