mRNA fraction of scRNA-seq from cancer cell lines
PC-3 cells and MDA-MB-231 cells were nanopore-electroporated to import DNA-tagged dextrans and hashtag oligonucleotides at an applied voltage of 75 V and 40V, respectively. The nanopore-electroporated PC-3 and MDA-MB-231 cells as well as non-electroporated MCF10A cells were pooled in PBS supplemented with 0.04% bovine serum albumin. The libraries of pooled cells were processed using Chromium Net GEM Single Cell 3 v3.1 and Chromium Controller (10x genomics).