Control
The extracted DNA was used to amplify the V3-V4 16S rRNA regions by PCR using the Bakt_341F and Bakt_805R primers and Herculase II Fusion DNA Polymerase. Nextera DNA libraries were prepared using the PCR products, and amplicon sequencing was performed on a MiSeq Illumina platform following a standard protocol for paired-end reads of 300 nucleotides.