mRNA fraction of scRNA-seq from cancer cell lines
PC-3 cells were treated with 10 M CytochalasinD in PBS for 2 hr and then nanopore-electroporated at an applied voltage of 40 V to import DNA-tagged dextrans and hashtag oligonucleotides. The nanopore-electroporated PC-3 cells as well as non-electroporated MCF7 cells were pooled in PBS supplemented with 0.04% bovine serum albumin. The libraries of pooled cells were processed using Chromium Net GEM Single Cell 3 v3.1 and Chromium Controller (10x genomics).