RGPD4-1
Total RNA was extracted using TransZol Up Plus RNA Kit (Cat#ER501-01, Trans) following the manufacturers instructions and checked for RNA integrity by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US). Qualified total RNA was further purified by RNAClean XP Kit (Cat#A63987, Beckman Coulter, Inc. Kraemer Boulevard Brea, CA,USA) and RNase-Free DNase Set (Cat#79254, QIAGEN, GmBH, Germany). Sequencing libraries were constructed following steps from the manufacturers instructions (VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina, Vazyme, NR604-02), followed by paired-end (2x 150bp) RNA-seq by Illumina HiSeq 2500.