Long read-based sequencing of the human telomeric transcriptome
We extracted nuclear RNA from HeLa, HEK293T or U2OS cells. We used CCCTAA(x5) biotinylated oligonucleotides to purify TERRA molecules. We generated cDNA using a CCCTAA oligonucleotide a sequenced it using the Native Barcoding Expansion kit from Oxford Nanopore technologies (EXP-NBD104). Sequencing was performed using the MinION device with the flow cells MK 1 R9 version (Oxford Nanopore).