WGS of Fusarium indicum
Library construction was done using the QIASeq FX DNA library preparation protocol (Cat#180475) as per the manufacturer's instructions. 50 ng of Qubit quantified DNA was enzymatically fragmented, end-repaired, and A-tailed in the one-tube reaction using the FX enzyme mix provided in the QIASeq FX DNA kit. The end-repaired and adenylated fragments were subjected to adapter ligation, whereby the index incorporated Illumina adapter was ligated, to generate sequencing libraries. These libraries were subjected to 6 cycles of Indexing-PCR [Initial Denaturation at 98C for 2 min, cycling (98C for 20 sec, 60C for 30 sec, 72C for 30 sec) and final extension at 72C for 1 min] to enrich the adapter-tagged fragments. Finally, for purification of the amplified libraries JetSeq Beads were used (Bio-68031). Further, quantification of the sequencing library was carried out by Qubit fluorometer (Thermo Fisher Scientific, MA, USA).