Bulk RNA sequencing on G1 phase sorted mESCs cells Control Rep3
In this study we have created dual sgRNAs based CRISPR knockout (KO) of the TOBF1 gene. Knockout cells were bulk sorted in G1 and non-G1 cell cycle phase by using ES-FUCCI system.For RNA sequencing on G1 phase sorted cells well used for both control and TOBF1 KO. Total RNA isolation was done for the sorted cells and sent for Hi-Seq Illumina sequencing.