16S rRNA amplicon analysis of oil-contaminated sandy beaches
Brine/concentrated seawater was filtered onto a 0.22 um filter at the beginning of the experiment. DNA was extracted using the Quick-DNA Fecal/Soil Microbe MidiPrep kit from Zymo Research and the V4-V5 region of the 16S rRNA gene was amplified with fusion primers. Clean products were pooled and loaded onto an Illumina MiSeq and sequenced using the 500-cycle MiSeq Reagent Kit v2 from Illumina.