RNA-Seq of Shorea curtisii: leaf at inflorescence stage (1 month before flowering time)
Poly(A)-containing mRNA were purified using oligo-dT beads before fragmented into smaller pieces. The first strand cDNA was synthesized using First Strand Master Mix and SuperScript II Reverse Transcriptase followed by the synthesis of the second strand cDNA using Second Strand Master Mix (Invitrogen, USA).