DNA-Seq of Sidalcea hendersonii: hermaphrodite individual
NEXTflex (Bioo Scientific Crop, Austen, TX, USA) was used for library construction. Fragment selection (400 bp) used Agencourt AMPure Xp magnetic beads (Beckman Coulter Genomics, Danvers, MA, USA). The two libraries were each sequenced on a single lane using 0.2 flow cells on an Illumina HiSeq-2000 sequencer generating 100 bp paired-end reads, giving a raw yield of 45 Gb per library