RNA-seq of saline spring sediment
RNA was extracted from sediment with ZymoBIOMICS DNA/RNA Miniprep Kit. Ribosomal RNA was depleted with the New England BioLabs NEBNext rRNA Depletion Kit (Bacteria) and a cDNA sequencing library was prepared with the New England BioLabs Ultra II RNA Library Prep Kit. Libraries were sequenced at The Centre for Applied Genomics (Toronto, Canada) on a NovaSeq 6000 (Illumina) with an S Prime 100-cycle flow cell.