40S rRNA amplicon: adult clam gill
DNA from each sample was extracted using the CTAB/SDS method. The variable V4V5 region of the bacterial 16S rDNA sequence was amplified with the barcoded universal bacterial primer pair of 515F and 907R using 10 ng of DNA as the template.The library was sequenced on the Illumina HiSeq2500 platform, and 274 bp paired-end reads were generated.