whole gneome sequence of homo sapiens: fetal patient brain diencephalon
1ml mix welled blood sample was added into 15ml RNase free centrifugal tube with 3 times erythrocyte lysate and reverse mixing. After repeatedly reverse mixing for cell lysis at room temperature for 10 minutes, centrifugation for 3 minutes with 2000RPM, then collect the precipitate. Vortices or flicks loosen and resuspend leukocyte precipitation, extracted DNA with whole genome extraction kit, fragmentation with ultrasonic, repair the fragment end with adenine. Finally, amplification library with Illumina sequencing adaptor, and sequencing.