RNA-seq of Homo sapiens: HFF
Total RNA from cell pellets was collected using Macherey-Nagel Nucleospin RNA purification kit and treated with DNAse I to remove DNA contamination. mRNA purification and cDNA synthesis was performed using NEBNext Ultra II Directional RNA Library Prep kit. NEBNext multiplexing illumina adapters were ligated to each sample and the libraries amplified using KAPA HiFi HotStart Ready Mix.