RNA-Seq-chicken adipose tissue
TRIzol kit (Invitrogen, CA, USA) was used to extract the total RNA from the twelve abdominal adipose tissue samples for RNA sequencing, according to the manufacturers guidelines. A total of twelve cDNA libraries were constructed from D, C, B, and A groups by using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs Inc., MA, USA), while small RNA libraries were constructed using NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs Inc., MA, USA).Libraries were sequenced with paired-end reads based on the Illumina sequencing platform by Suzhou PANOMIX Biomedical Tech Co., LTD (Suzhou, China).