noShrub_fertilizer_rhizosphere_rainySeason_rep2
DNA was extracted via the PowerSoil Pro and Plant Mini DNA extraction kits (Qiagen) according to manufacturers instructions, precipitated with sodium acetate, and transported to the US. There, samples were reconstituted and quantified via Qubit. DNA libraries were prepared at the Department of Energy Joint Genome Institute. 0.2 ng of Genomic DNA was sheared to 300 bp using the Covaris LE220-Plus and size selected with SPRI using TotalPure NGS beads (Omega Bio-tek). The fragments were treated with end-repair, A-tailing, and ligation of Illumina compatible adapters (IDT, Inc) using the KAPA-HyperPrep creation kit (KAPA Biosystems) and 5 cycles of PCR was used to enrich for the final library.  Illumina NovaSeq Sequencing was also performed at the DOE/JGI. The prepared libraries were quantified using KAPA Biosystems' next-generation sequencing library qPCR kit and run on a Roche LightCycler 480 real-time PCR instrument. Sequencing of the flowcell was performed on the Illumina NovaSeq sequencer using NovaSeq XP V1.5 reagent kits, S4 flowcell, following a 2x151 indexed run recipe. This generated 1.3 TB of sequencing, roughly 20 GB per sample.