Whole-genome sequencing of Pinus contorta
For WGS library construction, we used the Annoroad Universal EZ DNA Library Prep kit for MGI V1.0, Annoroad Adapters for MGI Set-T (N1-N12), and Annoroad DNA Cyclization kit for MGI V1.0 according to the manufacturers instructions. The genomic DNA was fragmented and selected with beads using Annoroad Universal EZ DNA Library Prep kit for MGI V1.0. The fragmented DNA was then repaired to obtain a blunt end and ligated with adapter. The ligation product was then amplified and subjected to the single-strand circularization process using Annoroad DNA Cyclization kit for MGI V1.0. The DNA library was sequenced by the DNBSEQ-T7 with a PE read length of 150 bp.