Metagenomic sequencing of bat: oropharyngeal swab
RNA extraction was carried out using the QIAamp Viral RNA mini kit (Qiagen, Germany) according to the manufacturer's protocol. Single-strand cDNA synthesis was performed with reverse transriptase M-MuLVRH (Biolabmix, Russia), and double-strand cDNA synthesis was carried out using Klenow fragment (SibEnzyme, Russia). Library preparation was performed using LIB Display kit (DNA Display, Russia) with fragmentation of cDNA to 2x300 bp fragments. Sequencing was performed with Illumina MiSeq (Illumina, USA) with generation from 40 million paired reads per sample.