Actinobacterial diversity of a developing peatland based on 16S rRNA gene amplification: Area 2 Middle Sample 3
Three sites were selected for sampling. From each site, three samples (1m apart) were collected. For each sample, soil was collected from three levels: top (first 5cm), middle (10-15cm), and deep (20-25cm) (total of nine samples per site; 27 samples in total). Metagenomic DNA (mgDNA) was isolated (from 0.1g of each sample) using the DNeasy PowerSoil DNA Isolation Kit (Qiagen), according to the manufacturers instructions. Metagenomic DNA was extracted using the Powersoil kit and subjected to 16S PCR. Amplicons generated were submitted for Iontorrent Sequencing