Sequencing of the fungal ITS amplicon from mock community ( ATCC Mycobiome Genomic DNA mix1)
The extracted DNA from know 10 Fungal strains were used to construct the ITS (Internal Transcribed Spacer) libraryfollowing the protocol from the Earth Microbiome Project. The amplified DNA was normalized using the SequalPrep Normalization Plate (Invitrogen) and pooled to form a single library. The ITSamplicons were sequenced using Ilumina MiSeq (2x250, paired-end).