scRNA_D43
Cells were dissociated using TrypLE Select, and resuspended in FACS buffer with SYTOX Blue Dead Cell Stain (Thermo Fisher Scientific). Live cells were sorted using FACSAria II (BD Biosciences) at Stanford Shared FACS Facility and collected in the FACS buffer supplemented with 10% fetal bovine serum (Thermo Fisher Scientific) and 2X Anti-Anti on ice. The sorted cells were pelleted, washed with cold 0.04% BSA in PBS, and resuspended in cold 0.04% BSA in PBS for cell number counting. Cell alive rate was determined using Trypan Blue. Only samples with greater than 90% cell alive rate were further processed. Cells were then subjected to 10X Chromium scRNA-Seq based on the manual.