16S sequencing of human fecal bacteria in different sugary types: GlucosePreA6
DNA were extracted from fecal samples and the 16S rRNA gene V4 variable region PCR primers 515/806 were used in a single-step 30 cycle PCR using the HotStarTaq Plus Master Mix Kit (Qiagen). Sequencing was performed using an Ion Torrent PGM following the manufacturers guidelines.