DTD fraction of MCF10A_07 cells
MCF10A cells were nanopore-electroporated at an applied voltage of 40 V to import DNA-tagged dextrans and FITC-BSA. Nanopore-electroporated single cells were sorted into 96-well plates containing lysis buffer (Takara) and barcoded oligo-dT primers using a FACSAria (BD Biosciences). mRNAs in single cells were reverse-transcribed with template-switch oligos and anchored-poly(dT)28 primers, flanking 8-bp UMI, 10-bp cell barcode, TruSeq read 1 sequence, and PCR handle. After digesting residual primers by exonuclease I, the full-length cDNA was amplified by 15 cycles of PCR. The PCR products from 16 wells were pooled and purified by SPRIselect beads (Beckman Coulter). The library was amplified by 1xKAPA Hifi Hotstart Ready Mix (Roche) and sequenced by HiSeq X (Illumina) instrument with 2 x 150 bp paired-end reads.