RNA-Seq of Saccharomyces cerevisiae: -DCL4 +SSC
Total RNA was prepared by the hot-phenol method and resuspended in RNase-free water, and 2 micrograms of RNAs were used for sRNA and transcriptome analyses. Libraries for RNAseq were prepared using TruSeq Stranded Total RNA Prep kit (Illumina) and sequenced by Illumina NovaSeq 6000.