RNA-Seq of vespa:larvae just beginning to develop, larvae starting to gut develop, adults that have completed a new metamorphosis, and fully transformed adult
According to the requirements of manufacturers, total RNA was extracted from different growth stages of V. mandarinia using TRIzol Reagent (Invitrogen). For each RNA sample, the concentration and purity of the extracted RNA were detected by Nanodrop 2000, the integrity of RNA was detected by agarose gel electrophoresis, and the Rin value was determined by Agilent 2100. The RNA sample of each different growth stages was used for cDNA library preparation. Library construction was completed by Origin (China), and libraries were sequenced by Illumina HiSeq 2002 platform at Origin (China). Three repetitions for each sample.