Clinical metagenomics of human CSF
Total nucleic acids were extracted using a NucliSENS easyMAG. Total extracted nucleic acids were split 1:1 and one fraction was DNAse treated followed by RNA purification. cDNA was generated from the purified RNA and pooled with the other total nucleic acid fraction. Illumina DNA Prep was used to generate a library from the pooled cDNA and total nucleic acids.