Cuscuta vs Solanum pennellii LA0716: attaching 1
Tissue samples were homogenised using 3 mm tungsten carbide beads in a TissueLyser II (Qiagen). Total RNA extraction was performed with the Maxwell 16 LEV Plant RNA Kit and Maxwell 16 Instrument (Promega). RNA concentration and quality were measured with a Bio-Rad Experion automated electrophoresis system using an RNA standard sensitivity kit. Library construction and mRNA sequencing were performed by Novogene. Sequencing libraries were generated using NEBNext UltraTM RNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturers recommendations and ~100M paired-end reads (150 bp in length) per sample were produced on a Illumina HiSeq4000 sequencing platform.