UKF_CDDP_5FU_DNA
All laboratory work was performed according to manufacturers instructions. Representative tumor material of the primary tumor was macrodissected. The cell lines were formalin fixed and paraffin embedded and taken as whole slides. The purification of DNA and RNA from FFPE tissue samples was performed using the Maxwell RSC Instrument (Promega Corporation, Madison, Wisconsin, USA) with the Maxwell RSC FFPE Plus RNA Kit (Promega Corporation, Madison, Wisconsin, USA) and QIAamp DNA FFPE Tissue Kit (Qiagen, Hilden, Germany), respectively.   The concentration of DNA and RNA was measured with the Qubit 4 Fluorometer (Invitrogen, Thermo Fisher, Waltham, Massachusetts, USA). For the library preparation 20 ng for the OncomineTM Comprehensive Assay v3 (Thermo Fisher, Waltham, Massachusetts, USA) were used. A 540 chip was applied.