gDNA of WT M. smegmatis
The paired-end sequencing library was prepared using Truseq Nano DNA Library prep kit. The library preparation process was initiated with 200 ng g-DNA. The g-DNA was mechanically sheared into smaller fragments by covaris followed by continuous step of end-repair where an A is added to the 3 ends making the DNA amplification step was performed using HiFi PCR Master Mixfragments ready for adapter ligation. Following this step, platform-specific adapters are ligated to both ends of the DNA fragments. These adapters contain sequences essential for binding dual-barcoded libraries to a flow cell for sequencing, allowing for PCR amplification of adapter-ligated fragments, and binding standard Illumina sequencing primers. To ensure maximum yields from limited amounts of starting material, a high-fidelity amplification step was performed using HiFi PCR Master Mix.