Amplicon of soiI bacteria in pasture subsample 2
The bacterial DNA was extracted from 0.5 g of soil sample by using a Fast DNA SPIN Kit for Soil (MP Biomedicals, USA) according to manufacturer's instructions. DNA concentration was determined by Qubit Fluorometric Quantification (Thermo Fischer Scientific , USA). Nucleic acid sequencing data output was obtained by paired end Illumina HiSeq Next Generation Sequencing (NGS). Sequencing covered 100 000 reads on each sample with a minimum reading length of 300 bp. Primer sequences Bakt_341F: CCTACGGGNGGCWGCAG and Bakt_805R: GACTACHVGGGTATCTAATCC were used to target V3 and V4 hypervariable regions of the 16s rRNA gene.