Shotgun-Seq of Chicken Manure
Total genomic DNA was extracted from 0.1 g of each sample by using NucleoSpin Soil mini kit (MACHEREY-NAGEL, Germany). The concentration and quality of the extracted DNA were determined by spectrophotometric analysis (NanoDrop 2000c, Thermo Scientific, USA) and agarose gel electrophoresis, respectively. Library preparation and metagenomics DNA sequencing of the samples were performed by Novogene Bioinformatics Technology Co, Ltd. Briefly, the DNA samples were randomly sheared into short fragments and end repaired. Poly-A tail and Illumina adapter were then added to the fragments (Illumina Inc., San Diego, CA, United States). The libraries were checked with Qubit and quantified by real-time PCR. Size distribution detection of the libraries was performed using bioanalyzer. Quantified libraries were then pooled and sequenced on Illumina platforms.