rna sequence of homo sapiens: fetal patient brain diencephalon
Total RNA was extracted from the dissected tissue using TRIzolTM Reagent (15596026, Invitrogen, USA). The strand-specific sequencing RNA-seq library was constructed in accordance with the manufacturers protocol using the VAHTSTM Stranded mRNA-seq Library Preparation Kit for Illumina (NR602-01, Vazyme, China).