ATAC-seq of mus musculus: 3 weeks female  NSN oocyte
GV oocytes washed with PBS were lysed by nuclei EZ prep (Sigma, NUC101). The nuclear extract without mitochondria was separated by a filter membrane (Millipore, RNWP02500) with a 1.4m pore and centrifuged at 12000g for 5min at high speed. ATAC-seq samples were constructed according to the instructions using TruePrepTM DNA Library Prep Kit V2 for Illumina (Vazyme, TD502-01), VAHTSTM DNA Clean Beads (Vazyme, N411-01) and TruePrepTM Index Kit V2 for Illumina (Vazyme, TD202).