Nasal fungal ITS amplicons obtained from healthy and BRD-positive pen-mates Holstein steers
Nasal swabs were collected from apparently healthy (n=73) and BRD-affected (n=56) pen-mates Holstein steers. Nasal swabs were collected from July-December 2020 in a feedlot located at Indiana, USA. DNA was then extracted from the nasal swabs to construct the fungal ITS (Internal Transcribed Spacer) library following the protocol fromthe Earth Microbiome Project. The amplified fungal DNA was normalized using the SequalPrep Normalization Plate (Invitrogen) and pooled to form a single library. The ITS amplicons were sequenced using Ilumina MiSeq (2x250, paired-end).