PromethION sequence of Salmonella enterica CVCC519
The large size fraction of DNA was selected by automated DNA size selection system (Blue Pippin, Sage Science); The adapter ligation was performed by using ligation sequencing kit (NBD103 and NBD114, Oxford Nanopore Technologies); The DNA library was quantified by Qubit; the real-time single molecule sequencing was performed on Nanopore PromethION sequencer.