Antibiotic Engraftment
The V4 region of the 16S rRNA gene was amplified and sequences were obtained by Illumina MiSeq via the Next Generation Sequencing Core in the Biosciences Division at Argonne National Laboratory (PMCID: PMC5069754 ). The primers used for amplification (515F-806R) contain adapters for MiSeq sequencing and single-end barcodes allowing pooling and direct sequencing of PCR products (PMCID: PMC3400413).