mRNA-seq of Drosophila melanogaster: female heads expressing Httex1.Q25
polyA-RNA were prepared from 1000 ng total RNA samples using NEBNext Poly(A) mRNA Magnetic Isolation Module then strand specific, indexed RNA-seq libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) with NEBNext Multiplex Oligos for Illumina.