16S rDNA of bacteria and archaea from the Willamette River
Whole-water (>0.2 m) gDNA from combined duplicate samples was amplified in triplicate using primers 515F-Y and 926R. Amplicons were gel extracted inclusive of the ~400-700 bp bands. Amplicons were sequenced by Azenta US Inc. using their Amplicon-EZ service; note that this combination of methods generated abundance chimeras.