16S rRNA amplicon analysis of oil-contaminated sandy beaches
Column microcosms of coastal seawater-brine mixture and sand amended with 1mL/kg of Hibernia crude oil, 10mgN/L by adding NH4NO3, 2mg/L KH2PO4 and with 90g/L of salt after 180 days of incubation. DNA was extracted using the Quick-DNA Fecal/Soil Microbe MidiPrep kit from Zymo Research and the V4-V5 region of the 16S rRNA gene was amplified with fusion primers. Clean products were pooled and loaded onto an Illumina MiSeq and sequenced using the 500-cycle MiSeq Reagent Kit v2 from Illumina.