Mt Genome of Xeruca formosensis: walking leg
A total amount of 200 ng DNA per sample was used as input material for the DNA library preparations. Sequencing library was generated using TruSeq Nano DNA HT Sample Prep Kit (Illumina USA) following manufacturers recommendations and index codes were added to each sample. Briefly, genomic DNA sample was fragmented by sonication at 350 bp. Then DNA fragments were end polished, size selected, A-tailed, and ligated with the full length adapter for Illumina sequencing, followed by further PCR amplification. After PCR products were purified (SPRIselect reagent, Beckman), libraries were analyzed for size distribution by Agilent 2100 Bioanalyzer and quantified by Qubit. The DNA libraries were sequenced on Illumina Miseq and 150 bp paired end reads were generated.