16S rRNA amplicon analysis of oil-contaminated sandy beaches
Column microcosms of coastal seawater-brine mixture and sand amended with 90g/L of salt after 90 days of incubation. DNA was extracted using the Quick-DNA Fecal/Soil Microbe MidiPrep kit from Zymo Research and the V4-V5 region of the 16S rRNA gene was amplified with fusion primers. Clean products were pooled and loaded onto an Illumina MiSeq and sequenced using the 500-cycle MiSeq Reagent Kit v2 from Illumina.