WGS of Scenedesmus obliquus DOE0152z
Large Insert SMRT bell library was prepared according to the Pacific Biosciences protocol. Since the DNA fragments were already 20-60 kbp in length, no additional shearing was required. Diluted SMRT bell/ DNA polymerase complex was sequenced on two SMRT cells 1M using the sequencing plate 3.0 and 10 hour movies.