soil vegas amplicon library
Total metagenomic DNA was extracted starting with 0.25 g of homogenized soil sample of each vega, using DNeasy PowerSoil Kit (Qiagen, Hilden, Germany). The V3-V4 hypervariable regions of the 16S rRNA gene were amplified from total extracted DNA using the forward primer Bakt-341 (5- CCTACGGGNGGCWGCAG-3 and reverse primer Bakt-805 (5- GACTACHVGGGTATCTAATCC-3) (Klindworth et al 2013).