BS-seq of Arabidopsis thaliana: WT Seedling
200 ng genomic DNA from micro dissected shoot apices from 2 to 3-day-old WT seedlings was subjected to fragmentation. The fragments were end repaired, ligated to Enzymatic Methyl (EM)-seq adapters and excess adapter removed using AMPure XP purification beads. This was followed by two sets of enzymatic conversion steps using the Enzymatic Methyl-seq kit to differentiate cytosines from 5mC and 5hmC. The resulting converted sequences of the bisulfite-treated adapter ligated library were PCR enriched and clean-up of library products. Prepared library was 150bp paired-end sequenced on Illumina NovaSeq 6000.