Amplicon (V4 region) sequencing of seed bacteria: seed biological replicate 31
Total DNAs were extracted from a single seed using SPINeasy DNA KIT for Soil (MP Biomedicals, Ohio, USA). The bacterial amplicons were amplified using the 515f/806r primer set with PCR blockers that inhibit the amplification of plant plastidial and mitcondrial DNAs. Library was constructed using Nextera XT Index kit (Illumina, California, USA).