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method of targeted next-generation sequencing was applied on the Illumina MiSeq platform using the AmpliSeq protocol with a custom panel containing primers for the synthesis of 661 amplicons covering the protein coding region of 21 candidate genes, including their UTR regions: BRCA1, BRCA2, BARD1, BRIP1, CDH1, CHEK2, EPCAM, MLH1, MRE11A, MSH2, MSH6, MUTYH, NBN, PALB2, PMS2, PTEN, RAD50, RAD51C, STK11, TP53