scRNA-seq of  mus musculus:colon
Tissue was processed to prepare scRNA-seq libraries by using Chromium Single Cell 3 Reagent v3 Kits (10x Genomics, Pleasanton, CA, USA). Single cells were suspended in calcium- and magnesium-free PBS containing 0.04% weight/ volume BSA. Then, single-cell suspensions were loaded onto a Chromium Single Cell Controller Instrument (10XGenomics, Pleasanton, CA, USA) to generate single-cell gel beads in emulsions (GEMs).  After generation of GEMs, reverse transcription, cDNA PCR amplification and library preparation were performed accroding to the 10x Genomics 3 gene expression protocol. These libraries were pooled and sequenced by HiSeq NovaSeq platform (Illumina, San Diego, CA, USA) in 150 bp paired-end mode.