CUT&Tag Seq for H3K27ac of mus musculus: E9.5 embryo neural tissues
Pregnant mice were given BaP (CAS-No. 192-97-2, purity 98%, Sigma, USA) intraperitoneally, dissolved in corn oil, from E6.5 for three consecutive days at a dose of 250 mg kg-1. On E9.5, embryos were collected and carefully inspected for NTDs under a dissecting microscope. Five embryos were pooled as a sample for RNA-seq experiment. Embryonic neural tissues, from the most rostral aspect of the forebrain to the caudal aspect of the hindbrain, were excised. Total RNA was extracted from neural tissues using the TRIZOL method and the concentration was determined using NanoDrop 2000 (Thermo Fisher Scientific, USA). Library preparation was performed using the Illumina's NEBNext UltraTM RNA Library Prep Kit (Illumina, USA) and high-throughput RNA-seq was performed using the Illumina Novaseq 6000 platform (Illumina, USA) with 100 bp paried-end sequencing.