WGS of homo sapiens: male
Modified Illumina TruSeq DNA PCR-Free library preparation workflow with prolonged ultrasonic shearing and adjusted bead-based size selection procedure was applied to generate shortened double-stranded library fragments. Genomic DNA was dissolved in TE buffer and sheared using Covaris S220 with the settings of  peak incident power 175 W, duty factor 10 %, cycles per burst 200, temperature 7 C and treatment time 430 s. End-repair was processed using T4 DNA polymerase.