cfDNA WGS: Sample MAL0779
CfDNA was isolated from plasma using the MagMAX Cell-Free DNA Isolation Kit (Applied Biosystems). The concentration and size distribution of cfDNA were measured by Qubit (Invitrogen) and BioAnalyzer (Agilent), respectively. Samples were randomly distributed into different batches for library preparation and sequencing. Case and their matched controls were always put into the same batch. Library construction was performed on 1ng of cfDNA using the KAPA HyperPrep Kit (Roche) and NEXTFLEX Unique Dual Index Barcodes (300nM final concentration, PerkinElmer).