ITS2 gene for Apis dorsata
PCR products were purified using SPRI Bead (Oberacker et al. 2019) followed by index PCR toincorporate Nanopore dual index barcode. The barcoded libraries were pooled and gel-purified usingWizPrep Gel/PCR Purification Mini Kit (WizBio, Korea) according to the manufacturers instructions.Quantification of the pooled barcoded amplicons used Denovix high sensitivity and appropriate amount ofthe DNA (~ 200 pmol) were used as the input for library preparation using the Oxford Nanopore LSK110ligation kit. The final library was loaded onto a Nanopore Flongle R9.4.1 flowcell and sequenced for 24hours