WGS HiC Illumina data for Leucaena trichandra
Chromatin conformation capture data was generated using the Phase Genomics (Seattle, WA) Proximo Hi-C 2.0 Kit, which is a commercially available version of the Hi-C protocol {Lieberman-Aiden, 2009 #2345}. Following the manufacturer's, intact cells from two samples were crosslinked using a formaldehyde solution, digested using the Sau3AI restriction enzyme, and proximity ligated with biotinylated nucleotides to create chimeric molecules composed of fragments from different regions of the genome that were physically proximal in vivo, but not necessarily genomically proximal. Continuing with the manufacturer's protocol, molecules were pulled down with streptavidin beads and processed into an Illumina-compatible sequencing library. Sequencing was performed on an Illumina HiSeq 4000, generating a total of 143,083,951 150 bp PE reads