Visium_YY0-45_lane1
Human embryonic and fetal esophageal and stomach tissues were washed in cold HBSS. Whole esophageal tubes were cut into ~3-5 mm length pieces from proximal to distal. Fresh tissues were directly embedded in OCT on dry ice. 10 μm fresh frozen sections of OCT-embedded tissues were used for Visium profiling. Such sections were collected using cryostat, sealed in airtight 50 ml Falcon tubes, stored at -80 °C for downstream profiling.  Sequencing libraries were prepared using the Visium Spatial 3' v1 (CG000239_Rev F) protocol according to the manufacturer's manual.