RNA-Seq of vitis rotundifolia cv carlos: adult leaf
Approximately 0.1 g of leaf tissues were collected 14 days post-inoculation and used for DNA and RNA extraction. Total genomic DNA was extracted using a CTAB method and used for the detection of Xfsf using RST31/RST33 primer pairs as described by Minsavage et al. 1994. Total RNA was isolated from leaf tissues collected from control and PD-inoculated plants using the Spectrum plant total RNA kit protocol (Sigma-Aldrich, MO, USA). RNA quantity and purity were assessed with a Nanodrop 2000 spectrophotometer and a Qubit 2.0 Fluorometer (Thermofisher, Waltham, MA, USA), respectively, and RNA quality was evaluated using an Agilent 2100 Bioanalyzer (Agilent Tech, Santa Clara, CA, USA). RNA sequencing was performed on the Illumina NovoSeq 6000 platform (Illumina, San Diego, CA, USA).