PacBio Hifi sequencing of homo sapiens cell line: APD
High Molecular Weight (HMW) DNA for SMRT sequencing was extracted using the Nanobind CBB kit. High-fidelity SMRTbell libraries were prepared using the SMRTbell prep kit 3.0. HMW DNA was sheared with hydropores deriving from the Megaruptor 3 DNAFluid+ and the Megaruptor 3 shearing-kit to 16~21kb. Large-insert SMRTbell libraries for sequencing were achieved by using a size selection with a 10kb cut-off using the BluePippin system. CCS reads were generated using standard settings in SMRTLink v11.0 with min passes = 3 and min read quality = 0.99.