Cyprinus carpio skin mucus shotgun metagenome
Extractions were carried out using ZymoBIOMICS DNA/RNA miniprep kits. Bead homogenisation was performed using a Vortex Genie 2 with a bead size of 0.1 mm, a homogenisation time of 15 min at maximum speed, then the Zymo Research kits DNA purification protocol was followed. Pooled total DNA samples were used to construct libraries using the NEBNext Ultra II Library Prep Kit. Paired-end shotgun metagenome sequencing was performed on an NextSeq 550 sequencer using the NextSeq High Output Kit v2 sequencing reagent kit. Base-calling was performed using bcl2fastq software (version 2.17.1.14, Illumina).