Human: MCF7 cells: ER ChIP-Seq
libraries were prepared and sequenced on four lanes of an Illumina NextSeq v2.5 High Output (150 cycle) flowcell. Each library was prepared using the SMARTer ThruPlex DNA Library Prep Kit (Takara Biosciences). We performed QC and quantitation on the library pool using the Qubit dsDNA, Agilent Bioanalyzer DNA 7500 Chip, and Kapa Illumina Library Quantification qPCR assays. The sequencing format was single end 100 bp. Base calling was done by Illumina Real Time Analysis (RTA) v2 software.