WES of homo sapiens: adult female TNBC specimen
The library was prepared using KAPA Hyper Prep Kit and 100500 ng of sheared DNA according to the manufacturers instructions. Individual tumor adapter-ligated libraries were enriched into the exome capture reaction, and for germline each adapter-ligated library was pooled before proceeding to capture using Agilents SureSelect Human All Exon V6+custom probes capture library kit. Each library was pooled and sequenced on Illuminas NovaSeq 6000 (Illumina, San Diego, CA) using 300 cycle kit.