Xanthomonas phage vB_XciM_LucasX genome sequencing
The phage DNA was quantified with the Quant-iT Picogreen dsDNA assay kit (Life Technologies, USA). The shotgun genomic library was prepared using an Illumina Nextera DNA library preparation kit (Illumina, Inc., USA) with 40 ng of DNA. The resulting DNA fragment library was cleaned up with Agencourt AMPure XP beads (Beckman Coulter, Inc., USA). The average fragment size was 665 bp, as determined in a 2100 Bioanalyzer. Quantification of Illumina sequencing library with KAPA Library Quantification Kit, normalization, and sequencing was performed following standard protocols for sequencing in the Illumina MiSeq platform. The library was subjected to one run using the MiSeq Reagent kit v2 (500-cycle format, paired-end reads).