Transcriptome analysis of OSCC cells infected with lentivirus encoding CDK5RAP2 shRNAC48_r1
We performed RNA-sequencing of CDK5RAP2-knockdown and control cells and compared the gene-expression profiles: Relative to control cells, CDK5RAP2-knockdown cells showed a >2-fold expression upregulation and downregulation of 96 and 802 genes, respectively. The results of heatmap analysis indicated that certain genes associated with CSC signature and epithelial-mesenchymal transition or metastasis were downregulated in CDK5RAP2 knockdown cells, whereas genes associated with differentiation were upregulated.