mRNA fraction of scRNA-seq from mHSPCs
Bone marrow cells were isolated from mouse femur. Cells were respectively nanopore-electroporated at an applied voltage of 75 V to import DNA-tagged dextrans. The nanopore-electroporated MCF7 cells were then stained by Totalseq-A antibodies in PBS supplemented with 0.04% bovine serum albumin. The libraries of pooled cells were processed using Chromium Net GEM Single Cell 3 v3.1 and Chromium Controller (10x genomics).