Single cell transcriptomics reveals the heterogeneity of immune landscape of IDH- wild type high-grade gliomas
CAR T cells and GSCs were incubated at a 1:4 effector cell ratio for 48 hours prior to sorting. The cells were sorted as live CAR-positive cells and pelleted. Total mRNA was isolated from the CAR T cells and purified with the RNAeasy Animal Total RNA Isolation Kit (Beyotime, Cat# R0032) .