16S rRNA-seq of patient 3 normal adjacent tissue : Sample P3_NAT_16S
"DNA was extracted from tissue samples using QIAamp Fast DNA Tissue Kit (Qiagen) and for mRNA sequencing, RNA was extracted from tissue samples using RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. DNA and RNA yield was determined fluorometrically using the High Sensitivity dsDNA kit (Invitrogen) and RNA High Sensitivity kit (Invitrogen) respectively on Qubit 3.0 Fluorometer and purity was estimated spectrophotometrically by measuring absorbance on QuickDrop (Molecular Devices). For Amplicon Sequencing PCR amplification on target regions (amplicons), sequencing libraries preparation and sequencing was performed by Novogene."