Mycobacterium lepromatosis strain:FJ924
A metagenomic approach was used. The infected tissue was homogenized and treated with sodium hydroxide and N-acetylcysteine to enrich the acid-fast bacilli for decontamination and culture. A smear slide was also prepared for Kinyoun stain and microscopy. Due to exhaustion of the extracted DNA from fresh tissue, the smear slide was used as the last resort for genome attempt after 6 years of archive. Genomic DNA (~3 ng) was extracted from the slide, and a library was constructed with KAPA kit. The library was enriched by six PCR cycles, quantified, assessed for size distribution, and sequenced.