WGS of Scenedesmus obliquus DOE0152z
HiFi SMRT libraries were prepared according to the Pacific Biosciences protocol. The gDNA was sheared to the target size of 15 kbp on Megaruptor 2 and purified with AMPure PB beads. Diluted SMRT bell/ DNA polymerase complex was sequenced on two SMRT cells 1M using the sequencing plate 3.0 and 20 hour movies.