Time-resolved dual transcriptomics of Pseudomonas aeruginosa biofilm formation in cystic fibrosis
Sequencing was performed at the Centro Nacional de Anlisis Genmico (CNAG) using a 2500 HiSeq platform. A stranded total RNA-Seq was performed with a deep sequencing of >50 million reads per sample and a read length of 2x75bp. The Library Preparation was performed as the manufactors indicate including a step of ribosomal RNA (rRNA) depletion prior to RNA sequencing.