Direct RNA sequencing (ONT) of APOBEC1 knockout mouse RAW 264.7 cell line replicate 2
The ONT SQK-RNA001 kit was used for the Direct RNA library preparation starting from 200 ng of input polyA + RNA and following ONT instructions. The libraries were then sequenced on a MinION instrument using R9.4 flow cells. MinKNOW was used to generate fast5 files.