WGS_HiFi_CCS_Unio_delphinus
For PacBio sequencing, mantle tissue was sent to Brigham Young University (BYU), where high-molecular-weight DNA extraction and PacBio HiFi library construction and sequencing were performed, following the manufacturers recommendations (https://www.pacb.com/wp-content/uploads/Procedure-Checklist-Preparing-HiFi-SMRTbell-Libraries-using-SMRTbell-Express-Template-Prep-Kit-2.0.pdf). Size-selection was conducted on the SageELF system. Sequencing was performed on four single-molecule, real-time (SMRT) cells using Sequel II system v.9.0, with a run time of 30 h, and 2.9 h pre-extension. The circular consensus analysis was performed in SMRT Link v9.0 (https://www.pacb.com/wp-content/uploads/SMRT_Link_Installation_v90.pdf) under default settings.