Targeted nanopore sequencing of BOK circRNA-derived amplicons (LNCaP cell line)
Total RNA from LNCaP cell line was reversely transcribed using random hexamers. Next, multiple nested PCR assays using divergent primers, specific for each BOK exon, were performed. After library construction, 3rd generation (nanopore-based) sequencing was conducted using the MinION Mk1C platform and the Flongle adapter.