IODP Exp. 382 1Ma eukaryote sedaDNA
Frozen sediment, EDTA+Beads+Silica extraction, 20uL library input, blunt-end repair, adapter ligation, Bst fill-in reaction, 1st amplificiation (IS7/IS8) with 22 cycles, 2nd amplification (IS4/GaII-Index) with 13 cycles, cleanup with beads aiming for keeping only <500bp fragments, Tapestation and Fragment Analyzer quantification, multiplexing