Metagenome of fermented mustard:Kharoli of Lakhimpur Assam
Library prepared with NEBNext Ultra DNA library preparation kit. Amplicons end repared enzymatically and tailing done with dA-tail followed by ligationto adapter sequence. The adapter ligated fragments cleaned with SPRI beads. The cleaned fragmnets are indexed during PCR cycle to generate final library of pair-end sequence. Prepared libraries are sequenced on Illumina MiSeq to generate 2X250bp or 2X300bp reads.