ITS rRNA gene sequencing of forest soil DNA: sample CFO1-3
Libraries for sequencing of the fungal ITS2 region were prepared according to Illuminas 16S Metagenomic Sequencing Library Preparation guide, with the exception of using Qiagen HotStar Taq DNA polymerase for the first PCR (amplicon PCR). The template specific primers were (without the overhang adapter sequence): ITS9F (5- GAACGCAGCRAAIIGYGA-3) and ITS4R (5- TCCTCCGCTTATTGATATGC-3).