RNAseq of APOBEC1 knockout mouse RAW 264.7 cell line replicate 2
The Illumina libraries were prepared from 500ng of total RNA, using Illuminas TruSeq Stranded Total RNA Sample Preparation Kit (Illumina, San Diego, CA, USA), according to the manufacturers protocol. The cDNA libraries were then checked on the Bioanalyzer 2100 and quantified by fluorimetry using the Quant-iTTM PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific) on NanoDrop 3300 Fluorospectrometer (Thermo Fisher Scientific). Sequencing was performed on a NextSeq 500 platform using the paired-end approach (2  75 bp).