miRNA-Seq of human neural stem cells derived from cell line ESI-017, day 3
NEBNext Multiplex Small RNA Library Prep Set for Illumina (#E7300S, #E7580S Set1, 2; New England Biolabs) was used to prepare libraries for further sequencing according to the manufacturer's instructions. Briefly, 800 ng of total RNA were used to create size-selected small RNA libraries (size selection with 6% PAGE gel). The sequencing was performed with 2.0 pM library using the NextSeq 500/550 High Output Kit v2.5 (#20024906; 75 cycles; Illumina.