RNAseq of Z.mays WT B73 immature leaf tissue collected 54 days after germination treated with a 24 hour mock hormone treatment, bioreplicate A
Total RNA was collected from treated tissues using TRIzol, quantified using a QuBit BR-RNA kit, checked for quality using agarose gel elecrophoresis, then processed for mRNA RNA seq library construction using the NEBNext Poly(A) magnetic isolation module followed by the NEB Ultra II library prep kit with unique, compatible barcodes for 36-plex multiplexing. 8 cycles of PCR enrichement were used during libary amplification to reduce PCR bias. Library quality was verified by Agilent 2100 BioAnalyzer, then quantitated by qPCR. Libraries were added in equimolar quantities for one single NovaSeq 6000 SP PE150 lane.