Amplicon sequencing of Soybean MAP3K: Sample 8
A single plant was used for extraction of genomic DNA using the Edwards method. DNA samples were quantified using Qubit. Amplicons for 40 genes were amplified in a multiplex PCR per sample, while adding sample-specific indices (Floodlight Genomics, USA). Illumina sequencing adapters were ligated using the KAPA Hyper Prep PCR-free Kit according to manufacturer directions (Kapa Biosystems, USA) and libraries were paired-end sequenced on an Illumina HiSeqX instrument (Admera Health, USA). All read data were demultiplexed, barcodes and adapter sequences were removed, and reads were trimmed and merged.