RNA-Seq of Hypoaccumulator of hydroxychavicol
Two samples (3 g RNA/sample) of H22 and L18 were subjected to Illumina high-throughput sequencing. According to the manufacturers instructions, sequencing libraries were generated using the NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA). To make them amenable to sequencing, the samples were fragmented, end-repaired, and collected into adaptor-ligated libraries. cDNA fragments were selectively purified from the libraries with a preferable length of 150~200 bp using the AMPure XP system (Beckman Coulter, Beverly, USA). The final cDNA library was generated with the PCR enriched cDNAs and the quality of the library was evaluated using the Agilent Bioanalyzer 2100 system. After clustering through HiSeq PE Cluster Kit cBot-HS (Illumina), deep sequencing was performed using an Illumina HiSeq 3000'' platform, and 150 bp paired-end reads were ultimately generated.