Transcriptome of resistant populations of Tetranychus urticae
Total RNA was extracted from all the six resistant and susceptible mite samples with the RNeasy Lipid Tissue Kit (Qiagen, Germany) according to the manufacturers instructions after treatment with RNase- free DNase I (Qiagen) to eliminate genomic DNA. The concentration and integrity of the RNA were assessed with a Thermo Scientific NanoDrop 8000 Spectrophotometer and Agilent 2100 Bioanalyzer, respectively (Agilent Technologies, USA). RNA with an OD260/280 1.8 and anRNA integrity number 5.0 was used in subsequent experiments. The high-quality RNA from each tissue sample was subjected to library preparation.The cDNA library was prepared with ~1.0 g of total RNA according to the IlluminaTrueSeq RNA Sample Preparation Kit (Illumina) protocol. The library was then amplified, the final library yield was recorded, and the resulting library was subjected to the paired-end sequence. The final yield was ~400 ng of cDNA, with an average fragment size of 300~350 bp. The resulting cDNA libraries (of all six samples) were then paired-end sequenced (2  150 bp) with NovaSeq 6000 (Illumina).