RNA-Seq of body wall: sea cucumber
Total RNA was isolated and purified using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer's procedure. The RNA amount and purity of each sample was quantified using NanoDrop ND-1000 (NanoDrop, Wilmington, DE, USA). The RNA integrity was assessed by Bioanalyzer 2100 (Agilent, CA, USA) with RIN number >7.0, and confirmed by electrophoresis with denaturing agarose gel. The libraries were constructed using TruSeq Stranded mRNA LTSample Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions. At last, we performed the 2150bp paired-end sequencing (PE150) on an Illumina Novaseq 6000 (LC-Bio Technology CO., Ltd., Hangzhou, China) following the vendor's recommended protocol.