GSM8002875: STM3006_2; Mus musculus; RNA-Seq
Total RNA was isolated using TRIzol LS reagent (Invitrogen) according to manufacturer's instructions and ethanol precipitation. RNA concentration was quantified with a Qubit Fluorometer (Thermo Fisher Scientific). Extracted total RNA was processed to obtain polyA-tailed transcripts with Dynabeads (Invitrogen) according to the manufacturer's instructions. A target amount of 200ng was used to create a readable transcript library for a single flowcell (type FLO-MIN106) using a protocol based on Oxford Nanopore Technology's DirectRNA SQK-RNA002 kit protocol. Each sample was sequenced on a single flowcell with version R9.4.1 chemistry.