GSM6932746: HL-60, DMSO, 120 hours, replicate 2; Homo sapiens; ssRNA-seq
At 6, 72 and 120 hours, RNA was extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. RNA-seq libraries were prepared using the ScriptSeq v2 kit (EpiCentre). Total RNA was depleted using RiboZero Gold (EpiCentre) and purified using the MinElute RNA kit (Qiagen). All RNA-seq libraries were assessed on a BioAnalyzer 2100 (Agilent) for library quantification and quality control and sequenced on an Illumina HiSeq 4000 using single-end, 50–base pair sequencing.