GSM7011459: Somatic HDA-1 binding profile in hcf-1(pk924) mutant, germline-less glp-1 RNAi, rep2; Caenorhabditis elegans; OTHER
Worms were exposed to an SDS-DTT buffer to weaken their cuticle, then dounced in CUT&RUN wash buffer and the resulting worm/cell mixture was used for standard CUT&RUN protocol (See Emerson & Lee, 2022, Current Protocols, DOI: 10.1002/cpz1.445) CUT&RUN libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina with 14 PCR cycles for amplification, as previously described (See Emerson & Lee, 2022, Current Protocols, DOI: 10.1002/cpz1.445)