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cDNA library construction was performed by the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA). Messenger RNAs (mRNAs) were enriched from total RNAs of G. aureata using oligo (dT) magnetic beads and then fragmented into short nucleotides using the fragmentation buffer solution. The cleaved mRNA was transcribed into the first-strand cDNA using a random hexamer primer and buffer solution. The second-strand cDNA was subsequently synthesized using buffer solution, deoxyribonucleotide triphosphate, DNA polymerase I and Ribonuclease H. After the end repairing, dA-tailing and adaptor ligation, the products were amplified by PCR and purified using the QIAquick PCR purification kit (Qiagen, Valencia, CA, USA) to create the final sequencing library