GSM6429661: HD 3 [SI-GA-B3], scRNA-seq; Homo sapiens; RNA-Seq
Frozen PBMCs from 4 healthy and 4 SSc patient donors were sorted to obtain enriched pDCs (CD3-CD14-CD123+BDCA4+) and T/pDC cell depleted PBMCs (CD3-CD123-BDCA4-) fractions, the two fractions were then remerged at 1:2 ratio. Cell viability of all donors are above 85%. 8000 cells from each donor were loaded to 10x single cell microfluidic chip to obtain around 5000 recovery cells. Libraries were constructed by following the instructions of 10x company (Chromium Single Cell 3' Reagent kits v3). The 8 libraries were quantified by NEBNext Library Quant Kit (E7630S) and equally merged and sequenced by Novaseq.