Amplicon NGS of off target site in gene edited human HSPCs
DNA Library Preparation was done using NEBNext Ultra DNA Library Prep kit following the manufacturers recommendations (NEB). DNA amplicons were indexed and enriched by limited cycle PCR. DNA libraries were validated on the Agilent TapeStation (Agilent Technologies) and were quantified using Qubit 2.0 Fluorometer (Invitrogen) and multiplexed in equal molar mass