GSM8046355: Minipigno.557APP/PS1 so2709P5-γ inhibition (2uM); Sus scrofa; RNA-Seq
Porcine fibroblasts were obtained from ear biopsies from WT, PS1 and APP/PS1 Göttingen minipigs and cultured in porcine fibroblast medium (Dulbecco's Modified Eagle's Medium (DMEM; Sigma Aldrich), 10% FBS (Life Technologies), 1% P/S (P/S, 10,000 units/mL and 10,000μg/mL; Life Technologies), 1% Gln (2.92g/100mL) and 6.667ng/mL Fibroblast Growth Factor-basic (bFGF; Life Technologies)) in 5% CO2 in a humidified chamber at 37oC. Established fibroblasts were split in a 1:3 ratio every 3 days using 0.05% trypsin EDTA (Life Technologies). All fibroblasts used in this study were tested negative for mycoplasma (MYCOPLASMACHECK, Eurofins Genomics). Library construction, sequencing and initial data filtering including adaptor removal were performed by BGI Europe Genome Center. Total RNA was subjected to oligo dT based mRNA enrichment. 50 bp single-end read sequencing was performed on BGIseq-500 with DNBseq technology. More than 20 million clean reads were obtained per sample.