GSM7178130: 22RV1, DMSO, rep3; Homo sapiens; RNA-Seq
Extracted via Rneasy Mini Kit (Qiagen, no. 74106) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using hexamers primers followed by second strand cDNA synthesis. The library was ready after end repair, poly A tailing, adapter ligation, size selection, amplification, and further purification.