GSM7179230: CaR, HSPC, Mut, Trx, SRCAP, rep1; Mus musculus; OTHER
50,000 sorted HSPCs from the bone marrow were permeabalized with 0.05% Digtonin to perform antibody binding and Mnase digestion. The antibody-enriched DNA fragments were released and used for library construction Cut-and-run sequencing library preparation was performed using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (New England Biolabs, E7645) according to the manufacturer's protocol.