18S amplicon metabarcoding of marine metagenome: Gulf of Mexico (USA)
Michigan State University Research Technology Support Facility Genomics Core no template negative control for 2nd PCR. Secondary PCR used dual-indexed, Illumina-compatible primers, targeting the Fluidigm CS1/CS2 oligomers at the ends of the PCR products. Sequencing runs were performed on an Illumina MiSeq to produce 250+250 nt paired reads.