GSM6729492: Skin dendritic cells, uncolonized, mRNA; Mus musculus; RNA-Seq
Skin was harvested and digested with collagenase. Cells were sorted to isolate dendritic cells (CD11c+ MHCII+) Library was prepared using Chromium Single Cell 3ʹ v2 protocol (10x Genomics). Cellular mRNA and antibody-derived oligos were reverse-transcribed and indexed with a shared cellular barcode. Indexed cDNAs were then pooled and amplified by PCR according to the Chromium Single Cell 3' v2 protocol (10x Genomics)