GSM6729651: Saline control, scRNAseq; Mus musculus; RNA-Seq
Female BALB/c mice were inoculated with 100,000 4T1 tumor cells and treated with either saline or PLG nanoparticles every 3 days starting 1 day after tumor inoculation. 14 days after inoculation, lungs were collected and pooled from n=4 mice per condition. Lungs were physically and chemically processed into a single cell suspension, treated with ACK buffer to remove red blood cells, and resuspended in DPBS supplemented with 2 mM EDTA and 0.5% BSA. Library preparation was performed by the University of Michigan Advanced Genomics Core following manufacturer instructions (Illumina NovaSeq 6000).