GSM7839370: SAM24402086_BRM_nodox; Homo sapiens; OTHER
Cultured cells were trypsinized into single cell suspensions, counted using the Vi-CELL XR cell counter (Beckman Coulter) and nuclei were isolated based on the 10x demonstrated protocol (CG000365_DemonstratedProtocol_NucleiIsolation_ATAC_GEX_Sequencing_RevC.pdf). The nuclei were processed and libraries were constructed using the Chromium Single Cell Multiome ATAC + Gene Expression Reagent Kit (10x Genomics, Cat. No. 1000285) as per the manufacturer's recommendations. Accessibility and expression libraries were profiled using the Tapestation High Sensitivity DNA kit (Agilent Technologies) and quantified with the Kapa Library Quantification Kit (Kapa Biosystems).