MinION sequencing
2g of leaf material was macerated in an ice-cold pestle and mortar with approximately 2mL of chloroplast isolation buffer (0.05M K2HPO4, 0.1M KH2PO4, 1.34mM KCl, 0.3M glucose). The resultant solution was passed through a coarse filter made using wash muslin cloth. The solution was centrifugated at 1000rpm for 30s, the supernatant was removed and centrifugated at 1000rpm for 7 min to obtain a pellet of chloroplasts. Approximately 0.01g of this pellet was taken forward to DNA extraction using the Qiagen DNeasy Plant kit. The DNA extracted was then sequenced using ONT Rapid Sequencing kit SQK-RAD004 and a MinION flow cell ï¿½(R9.4.1) FLO-MIN106D.