GSM7119208: HH9 Neural Fold scATAC-seq; Gallus gallus; ATAC-seq
Dorsal neural folds were manually dissected from HH9 embryos . After tissue collection, cells were dissociated in Accumax (Innovative Cell Technologies, AM105) for 20 min at RT under mild agitation and nuclei preparation was perfomed according to the 10X Genomics suggested protocol. Cells were lysed in cold lysis buffer containing 10mM Tis-HCl pH7.4, 10mM NaCl, 3mM MgCl2, 1% BSA, 0.01% Tween 20, 0.01% % Nonidet P40 Substitute, and 0.001% digitonin for 5 minutes on ice. Following cell lysis, cells were washed in 10mM Tis-HCl pH7.4, 10mM NaCl, 3mM MgCl2, 1% BSA, and 0.1% Tween 20 and resuspended in 1x Nuclei Buffer (10x Genomics, 2000153/2000207) at a concentration of 3,500 nuclei per uL. scATAC-seq libraries were generated via the Chromium Single Cell ATAC pipeline (10x Genomics, PN-1000175).