Of2 miRNA
The library was constructed using Small RNA Sample Pre Kit, using the special structure of the 3' and 5' ends of Small RNA (complete phosphate group at the 5' end and hydroxyl group at the 3' end), and using total RNA as the starting sample. Using the special structure of 3' and 5' ends of Small RNA (5' end has complete phosphate group and 3' end has hydroxyl group), we use total RNA as the starting sample, directly add the junctions on both ends of Small RNA, and then synthesise the cDNA by reverse transcription. cDNA is then amplified by PCR, separated from the target DNA fragments by PAGE, and then recovered by cutting the gel.