GSM6770005: RAW264.7, LPS+NaBu Treated_sample_1; Mus musculus; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes and cross-linking was done using glycine for 10 minutes. For nucleic preparation and chromatin digestion, two separate buffers were used containing DTT and micrococcal nuclease was used, followed by sonication. ChIP-seq libraries were performed using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, Cat# E7645L).