Total genomic DNA of Gomphonema parvulum
Colonies were scraped from agar plates using an inoculation loop and suspended in the extraction buffer provided in the Quick-DNA Miniprep kit manufactured by ZymoResearch (Irvine, CA). Total DNA yield was measured using a Nanodrop Lite (ThermoFisher, Waltham, MA) and the integrity of the DNA checked with agarose electrophoresis. Total DNA yield from the Georgia isolate was too low for whole genome sequencing so it was amplified using Qiagens REPLI-g Mini Kit (Germantown, MD, USA).  Whole genome Illumina MiSeq paired-end sequencing was provided by the commercial sequencing center, Genewiz (South Plainfield, NJ) using their Short-Read Non-Human WGS service.