Illumina HiSeq 2500 paired end sequencing; Transcriptome analysis of potential candidate genes and molecular pathways in Et-1-deficient mice with depigmentation
Transcriptome analysis of potential candidate genes and molecular pathways in Et-1-deficient mice with depigmentation
Skin tissues were collected from WT mice, and depigmented and pigmented skins of Ets-1 KO mice. Total RNA was harvested using Trizol reagent (Invitrogen) for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using SureSelect Strand-Specific RNA Library Prep Kit (Agilent) following manufacturer's protocols, followed by AMPure XP beads (Beckman Coulter) for size selection.