GSM7977438: T cells, Donor03, 72hrs; Homo sapiens; RNA-Seq
Total RNA was harvested from T cells using Rneasy mini plus kit (Qiagen) at different time points. 200 ng of total RNA mixed with 2 µl of External RNA Controls Consortium (ERCC) spike in RNA (Thermo Fisher Scientific, Germany) after 1:500 dilution was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using Illumina TruSeq Stranded Total RNA library prep workflow.