GSM6022185: Control 2; Danio rerio; RNA-Seq
Total mRNA was extracted from liver tissue of the zebrafish using Qiagen's miRNeasy Mini kit (Qiagen, Hilden, Germany). To prepare mRNA-Seq libraries the TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA, USA) was utilized; 100ng of total input Liver RNA was used in accordance with the manufacturer's protocol. High through-put RNA sequencing (RNAseq) was performed at the Queens University Belfast, Genomics Core Technology Unit on an Illumina Next SEQ 500 instruments with the mRNA library sequenced to a minimum depth of 50 million reads.