GSM7178289: Olfr78 WT colon cells; Mus musculus; RNA-Seq
Colon tissues from one wild type and one Olfr78GFP KO mice (respectively 8 and 6 months-old) were cut in small pieces and put to digest in collagenase I at 0,5 mg/ml for 25 min at 37°C under agitation at 75 RPM. Ups-and-downs were performed with a 10 ml pipette and the samples were put back 20 min at 37°C and 75 RPM. Equal volume of PBS-5 mM EDTA was added and cells were centrifuged 10 min at 50 g. The supernatant was removed and 5 ml of Trypsin were then added for 25 min at 37°C and 75 RPM. Cells were pipetted up and down, passed through a 40 µm filter and pelleted at 50 g for 10 min. The pellet was washed 3 times with PBS-BSA 0.04% (w/v). Library was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics).