GSM8046411: wt2, rep2; Schizosaccharomyces pombe; RNA-Seq
Total RNAs were extracted with hot acidic phenol method and then cleaned up with the RNeasy Mini kit (Qiagen, cat. # 74004) for further procedures. RNA-Seq libraries were prepared from total RNA using poly(A) enrichment of the mRNA to remove ribosomal RNA, and sequenced on an Illumina NovaSeq 6000 platform.