RNA-Seq of Drosophila Melanogaster Antenna: Wildtype_20DPE_S1
Antennae were homogenized using disposable RNAse free micropestles (USA Scientific) and QIAshredder columns (Qiagen). RNA was extracted using an RNAeasy Micro Kit (Qiagen) and digested with DNAse from an iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad). The Center for Genome Innovation at the University of Connecticut carried out quality control on the RNA samples (~0.5 g each) and generated libraries with the TruSeq Stranded mRNA library kit (Illumina). Trimmed reads were generated using Sickle with the default parameters. Trimmed reads were aligned to the reference genome with mitochondrial genes removed using STAR with the default parameters.