Bees cuticular ITS metagenome A. m. carnica2
Microbial community DNA was extracted from bees cuticular samples using the Quick-DNA Fecal/Soil Microbe Miniprep Kit according to manufactureres' instructions (Zymo Research, Freiburg, Germany).  ITS Amplicons of bees cuticular DNA samples  were generated with the primer set  fITS7 (5-GTGARTCATCGAATCTTTG-3) as suggested by Ihrmark et al. (2012) and ITS4 (5-TCCTCCGCTTATTGATATGC-3) as suggested by White et al. (1994).