GSM6025297: Prx1Cre;Rosa26-tdTomato bone marrow single cell RNA-sequencing; Mus musculus; RNA-Seq
Animals were euthanized according to IACUC protocol and hind limb muscles were isolated. Muscles were mechanically and enzymatically digested with Collagenase B/Dispase mixture for 30 minutes. Following a red blood cell lysis, cells were stained with DAPI and FACS sorted on tomato expression and submitted for 10X Genomics single cell isolation and barcoding. RNA libraries were prepared for sequencing using standard Illumina protocols