GSM7658859: Esophagus, EoE33, rep4; Mus musculus; RNA-Seq
Whole esophagi were collected in RNeasy, immediately flash frozen in liquid nitrogen, and shipped on dry ice to GENEWIZ for bulk RNA extraction. Total RNA was extracted from fresh frozen cell pellets using the Qiagen RNeasy Plus Universal mini kit. mRNA was enriched with polyA selection and sequenced using the Illumina HiSeq platform with a 2x150 paired end configuration.