GSM8047872: OCT_WT_545, ST; Mus musculus; OTHER
OCT: Fresh frozen tissue was embedded in OCT and sectioned at 10µm on a cryostat. All blocks had a RIN score >8.0. FFPE: 40μm (8 x 5μm sections) each from mouse spleen. The RNA was extracted using the Qiagen FFPE extraction kit and the DV200 assessed on the TapeStation. All blocks had a DV200 >50%. Spatial: Libraries were prepared with 10x Genomics Visium Spatial Gene Expression slides and reagent kit according to manufacturer's instructions. scRNA-seq: 10x scRNA-seq with the large BioLegend panel of antibody derived tags (ADTs) Spatial Transcriptomics and scRNA-seq