GSM7977417: S17-0386; Canis lupus familiaris; RNA-Seq
Tissue samples from canines with spontaneous meningioma were acquired as surgical biopsies or from immediate post-mortem necropsies and archived as formalin-fixed, paraffin-embedded (FFPE) samples at Texas A&M University between 2011 and 2022. Following histopathological diagnosis, 5-10 25um thickness scrolls were collected from each meningioma for RNA sequencing. Scrolls were deparaffinized with xylene at room temperature, re-hydrated with an ethanol series, and subject to total RNA extraction using TRIzol reagent (Thermofisher, manufacturer's instructions). RNA quantity and quality was evaluated on a TapeStation and all samples with a concentration of 10 ng/µl between 200-1,000nts were used for library preparation (n=62). Illumina library preparation was performed to manufacturer's specification and 150 nt paired-end sequencing was performed. RNA libraries were prepared for sequencing using standard Illumina protocols.Paired-end RNA sequencing was performed.