GSM7049581: Sublibrary 5, scRNAseq; Homo sapiens; RNA-Seq
To obtain a single cell dissociation, the papain dissociation kit was used according to the manufacturer's protocols (Worthington). Briefly, 2-3 organoids were initially broken into smaller pieces using a sterile razer before dissociation in papain solution. The cells were broken up further by gently pipetting using a 1000µL pipette. Barcoding and library preparation of single cell suspensions was performed according to the manufacturer's protocols (SplitBio). The cells were fixed and lysed, before multiple rounds of barcoding and cell measurements were employed to ensure the cells were in a uniform single cell suspension and each cell was given a unique barcode.