GSM6589610: CEBPB WT BAT mRNAseq after 4h cold stress, rep5; Mus musculus; RNA-Seq
Total RNA was extracted with PureLink RNA mini kit (Invitrogen) Libraries were prepared by the Van Andel Genomics Core from 500 ng of total RNA using the KAPA Stranded mRNA-Seq Kit (v4.17) (Kapa Biosystems, Wilmington, MA USA). RNA was sheared to 300-400 bp. Prior to PCR amplification, cDNA fragments were ligated to IDT for Illumina TruSeq UD Indexed adapters (Illumina Inc, San Diego CA, USA).