GSM6429544: mammay epithelial cells,peak lactation IP,P1; Capra hircus; OTHER
Total RNA was extracted using a TRIzol kit (Invitrogen, USA). Eukaryotic mRNA from the extracted total RNA was enriched using Oligo (dT) beads, and a Ribo-ZeroTM Magnetic Kit (Epicentre, USA) was used to remove rRNA and enrich prokaryotic mRNA. Then, the enriched mRNA fragments were divided into short fragments using fragment buffer, and the RNA was divided into 2 samples, 1 of which was used as the input control. The transcriptome sequencing library was constructed to eliminate noise during the capture of methylated fragments, another  RNA sample was enriched with an m6A-specific antibody.