GSM6730072: Muscle stem cells (MuSCs), DEN, rep 2; Mus musculus; RNA-Seq
MuSCs were FACS-isolated and sorted directly into lysis buffer containing β-mercaptoethanol from the RNeasy Micro Kit (Qiagen). Library preparation was performed with a two-step approach: 1) introducing 4 ng of total RNA to the SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (Takara) for full-length cDNA synthesis was followed by 2) introducing 300 pg of full-length cDNA to the Nextera XT DNA Library Preparation Kit (Illumina).