GSM6320840: RNAseq P. flava late gastrula replicate 2; Ptychodera flava; RNA-Seq
Total RNA of each developmental stage was isolated from one to three biological replicates using the RNeasy Micro Kit (Qiagen). Library preparation for stranded polyA-based mRNA was then performed using the TruSeq Stranded mRNA Library Prep Kit (Illumina).