GSM8047526: Abundance, FACS gate B rep 1 bio rep 2; synthetic construct; OTHER
Genomic DNA extraction was performed using Qiagen DNeasy blood & tissue kit (Cat. No. 69506) A site-saturation library of codon optimized human ASPA were custom synthesized and barcoded. Constructs of ASPA variants fused to GFP and an IRES-mCherry expression control were integrated into a HEK293T landing pad cell line