GSM8046877: Molino-3; Astyanax mexicanus; ncRNA-Seq
Small RNA (<200nt) was extracted using the mirVana RNA isolation kit (Catalog number: AM1560) according to the manufacturer's instructions Libraries were made according to the manufacturer's directions for the TruSeq Small RNA Library Prep Kit (Illumina, Cat. No. RS-200-0012) RNA-Resulting libraries were size selected for 145-160bp fragments using Novex 6% TBE gels (Invitrogen, Cat. No. EC6265BOX) to retain only the small RNA inserts Libraries were pooled, requantified, and sequenced as 75bp single reads on an Illumina NextSeq 500 instrument high-output flow cell