Whole Exome Sequencing of Mixed Human Cell lines: DEDA2 treatment
A mixture of the six liver cancer cell-lines was cultured in six-well plates (6x105 cells per well). Three wells were treated with 3L of DEDA (1 M). After 30 h, the cells were collected, and genomic DNA was isolated using the QIAamp DNA Mini Kit from QIAGEN. The whole exome sequencing (100x) was performed at Novogen.