GSM7089299: EZ TF Replicate 2; Homo sapiens; OTHER
gDNA from cells collected at initial (T0) and final (TF) timepoints (12 samples total) was harvested using Qiagen DNA Blood Maxi Kit Next Generation Sequencing of sgRNA sequences: sgRNA sequences were PCR amplified and library was constructed following the TKOv3 CRISPR Screen Protocol. 200ng of DNA from each sample was used for sequencing