ONT passed_fastq Sequencing of Ornithoptera alexandrae: larva female FC563 specimen
Tissues from the thorax or abdomen were used to extract high molecular weight DNA with the Qiagen genomic DNA kit. Whole-genome libraries were constructed using the resulting high-molecular-weight DNA as input for the Nanopore LSK-109 ligation kit (Oxford Nanopore Technologies, UK) following the manufacturers protocol. Long-read sequencing was performed on a GridION device with two R9.4.1 flow cells. Raw .fast5 reads were basecalled using ont-guppy/bin/guppy_basecaller ("-c dna_r9.4.1_450bps_sup.cfg -i ./all_fast5/ -s ./Guppy_5.0.15_basecall/ --compress_fastq --recursive --device cuda:all:100% --min_qscore 10")