GSM7119274: Veh_2; Homo sapiens; RNA-Seq
Total RNA from cells was extracted using the Norgen Total RNA Purification Kit (Norgen Biotek) following the manufacturer's protocol. DNase1 was applied during RNA extraction to remove genomic DNA. RNA was eluted in nuclease-free water, and the concentration was measured using Nanodrop (Thermo Fisher Scientific). After quality control by Agilent 2100 Bioanalyzer, the total RNA was used as input for library preparation by Novogene Co., Ltd, followed by high-throughput sequencing on Illumina HiSeq X with PE150 mode to produce approximately 20 M reads per sample.