RNA-Seq of Tachycineta bicolor: nestling liver
RNA purity and concentrations were determined by UV absorbance at 260 nm and 280 nm using a NanoDrop (Thermo, MA).RNA quality was assessed on a 2100 Bioanalyzer (Agilent, CA). For each library preparation, mRNA was purified from 1ug total RNA using poly-T oligo-attached magnetic beads and were prepared according the TruSeq Stranded mRNA Sample Preparation Guide (Rev. E) using the Illumina TruSeq Stranded mRNA Sample Preparation kits. Quality assessment and quantification was performed using KAPA Library Quantification Kits (Roche Sequencing Solutions, IN, USA) and the libraries were sequenced on an Illumina HiSeq 2500 to generate paired-end reads of 150-bases.