16S rRNA gene V1-V3 sequencing of buccal mucosa swab from proband: LKG-017
Amplification of 16S rRNA gene V1-V3 region using 12 phased primer pairs derived from 27Fmod: 5' AGRGTTTGATCMTGGCTCAG 3' and 519R: 5' GWATTACCGCGGCKGCTG 3' primers. 2nd Indexing PCR using Illumina Nextera v2 system