GSM6809953: H3K27me3_40K_rep1; Homo sapiens; OTHER
Target genomic DNA was extracted using the NovoNGS® CUT&Tag 3.0 High-Sensitivity Kit (NovoProtein, N259-YH01). The CUT&Tag assay was performed using the NovoNGS® CUT&Tag 3.0 High-Sensitivity Kit (NovoProtein, N259-YH01). 10,000 cells were washed twice with 1.5 mL of wash buffer and then mixed with activated concanavalin A beads. After successive incubations with the primary antibody (RT, 2h) and secondary antibody (RT, 1.5 h), the cells were washed and incubated with pAG-Tn5 for 1.5 h. Then, tagmentation buffer was added to activate tagmentation for 1 h. The tagment DNA fragments were purified using tagment DNA extract beads and washed by 80% ethanol. Then, the libraries were sequenced using the Illumina NovaSeq 6000 platform.