GSM7178158: Ctrl_Knic_1; Homo sapiens; OTHER
Take out the cryovial and shake it in 37°C water to quickly thaw it within 1-2min.Cells were centrifuged for 5 min at 600×g at 4 ℃ Cell activity was detected with LUNA-FL and counted.1 ml of 1 XHB buffer was added into the tissue sample, grinding and filtering, filtrate centrifuged at 4℃ 500×g for 5 min, supernatant was discarded, density gradient centrifugation for 10 min, nucleus suspension was obtained and counted with LUNA-FL. CUT&Tag library was performed as described previously [1]. Briefly, the cells are bound to Concanavalin A-coated magnetic beads, and the cell membrane is permeabilized by Digitonin. The enzyme pA-Tn5 Transposase precisely binds the DNA sequence near the target protein under the antibody guidance and results in factor-targeted tagmentation. DNA sequence is tagmented, with adapters added at the same time at both ends, which can be enriched by PCR to form the sequencing-ready libraries.