GSM8083523: scE14.5_2; Mus musculus; RNA-Seq
Both foregut and hindgut were collected from embryos (E14.5), rinsed in 1X PBS, and singularized into individual cells using Accumax and mechanical pipetting. Cells were then turned into scRNA-Seq libraries using standard 10X Genomics 3' scRNA-Seq protocols. Single nuclei were then isolated using 10X Chromium single nuclei isolation kit. 20k nuclei were then used as input into the standard 10X Genomics scRNA-Seq library pipeline. 3' 10X Genomics