ddRADseq of Chirostoma humboldtianum species complex
Library preparation was carried out following the double-digest RADseq (ddRADseq) protocol described by Peterson et al. (2012) using MsI and PsI restriction enzymes. We size-selected digested fragments of 300-600bp. Each library contained up to 20 individuals indexed by a set of six base-pairs barcodes in combinatorial schemes, and further sequenced using 100 base pair-ended sequencing.