GSM7839396: SAM24409811_KR_Rep2; Homo sapiens; ATAC-seq
cells were trypsinized, washed with 1× PBS, counted and processed for library prep About 100,000 cells were then lysed for 5 minutes on ice in 100 mL of Lysis buffer (10 mM Tris, pH 7.5, 10 mM NaCl, 3 mM MgCl2, 0.1% NP-40, 0.1% Tween-20 and 0.01% digitonin). 1 ml of wash buffer (10 mM Tris, pH7.5, 10 mM NaCl, 3 mM MgCl2, 0.1% Tween-20) was then added and the pellets were spun down at 500 g for 10 minutes at 4 °C. The supernatant was discarded, and the pellet was resuspended in 50 mL of tagmentation buffer (25 µL 2× TD buffer, 16.5 mL PBS, 0.5 mL 10% tween 20, 0.5 mL of 1% Digitonin, 2.5 µL Tn5 transposase and 5 mL H2O). The tubes were incubated at 37 °C for 30 minutes, followed by DNA isolation using the Qiagen MinElute Cleanup Kit (Cat. No. 28206). The DNA was then amplified using NEBNext 2× PCR Master mix (Cat. No. M0541L) and analyzed by Tapestation High Sensitivity DNA kit (Agilent Technologies) before submitting for sequencing.