GSM7711993: Adult MPP2 biol rep 3; Mus musculus; RNA-Seq
RNA was isolated from 5000 - 20,000 HSPCs using the RNeasy Plus Micro Kit (Qiagen, 74034). RNA integrity number (RIN) was determined by Bioanalyzer (Agilent Technologies) and RNA samples with RIN > 9.0 were further processed. RNA samples were processed over multiple experimental “sort days” and stored at -80°C in RNA isolation buffer until library preparation. A total of 3biological replicates were sequenced for each population. RNA was quantified and QC using an Agilent Bioanalyzer. Illumina sequencing libraries were prepared and sequenced at the NYU Genome Technology Center (New York, NY). The NuGen Ovation Trio low input RNA-seq library preparation kit was used for library preparation. All libraries were prepared at the same time in order to minimize batch effects. Libraries were sequenced using an Illumina NovaSEQ 6000 instrument with paired end 50 base pair sequencing to a target depth of 40 million unique reads per sample.