GSM8083012: 12week hetero; Mus musculus; RNA-Seq
Total RNAs from mouse skeltal muscle at the age of 1, 12 and 36 weeks were extracted by using RNeasy Plus Universal Mini Kit (Qiagen). Nine representative libraries were generated for the nine groups (Wild-type, Heterozygote and Homozygote of 1 week, 12 weeks and 36 weeks) by RNA-seq with SMART-seq v4 Ultra Low Input RNA kit for sequencing (Takara Bio Inc.) and Nextera XT DNA Library Prep Kit (Illumina, Tokyo, Japan) and sequenced on a NovaSeq 6000 instrument (Illumina). RNA libraries were prepared for sequencing using standard Illumina protocols.