GSM7119399: Ileal mucosal cells, Crohns Disease [Sc10]; Homo sapiens; RNA-Seq
Ileal biopsies obtained were protease digested, the recobered singel cells were processed for 10X Genomics 3' Single cell RNA sequencing with target of 10000 cells which are later sequnced at a read depth of 50,000 reads per cell Ileal surgical resections collected are cut into 10μm tissue sections that were processed using 10x Visium spatial transcriptomics for both H&E stain and Immunofluroscence using epithelial marker (E-Cadherin), immune cell marker from Cell Signaling (CD45/ PTPRC) and stromal marker α-Smooth muscle actin (α-SMA).