Identification of DNA viruses in American wigeon fecal sample
Viral DNA was extracted from the faecal sample (1g) using a High Pure Viral Nucleic Acid Kit (Roche Diagnostics, USA). Circular viral DNA was preferentially amplified by rolling-circle amplification (RCA), using a TempliPhi 2000 Reaction Kit (GE Healthcare, USA). The RCA product was used to prepare an Illumina sequencing library, which was then sequenced on a NovaSeq6000 (2x150bp).