RNA-Seq in feces: patients with non-alcoholic fatty liver
DNA extraction was performed using the MagPure Stool DNA KF kit B(Magen, China). PCR was performed to amplify the template in the DNA samples using V3V4 region primers, which included 341F (5-ACTCCTACGGGAGGCAGCAG-3) and 806R (5- GGACTACHVGGGTWTCTAAT-3). The validated libraries were used for sequencing using an Illumina MiSeq platform (BGI, Shenzhen, China). Then, amplicon sequences were merge-paired, quality filtered and analyzed using Quantitative Insights into Microbial Ecology (QIIME) software (QIIME2 v2019.84).