GSM7711228: con-2.Input; Homo sapiens; RNA-Seq
Total RNA was extracted from H460 cells using an ultrapure total RNA extraction kit (Simgen, Hangzhou, China). 1.3ug total RNA was used for the construction of sequencing libraries. The GenSeq® rRNA Removal Kit (GenSeq, Inc.) was used to remove ribosomal RNA (rRNA) from the sample. After the removal of rRNA, sequencing libraries were constructed using the GenSeq® Low Input RNA Library Prep Kit (GenSeq, Inc.) kit according to the manufacturer's instructions.