GSM5603564: day3_AHDCKO; Homo sapiens; RNA-Seq
Single cell suspensions were prepared with Accutase, and any large clumps were removed with 40μM cell strainers. Dead cells were eliminated with a dead cell removal kit (Miltenyi) per manufacturer instructions. 10,000 cells at a concentration of 1,000 cells per μL were resuspended in PBS with 0.04% BSA (Miltenyi). Library prep was performed with a Chromium Single Cell 3' Reagent Kit v3.1 (10x Genomics) per manufacturer instructions.