GSM6774411: SCM, replicate 3, snRNAseq; Mus musculus; RNA-Seq
Nuclei were isolated from frozen samples that were resuspended in TST buffer (146 mM NaCl, 10 mM Tris, 1 mM CaCl2, 21 mM MgCl2, 0.03% Tween20, and BSA) and pelleted by centrifugation. The nuclei pellets were resuspended in ST buffer (146 mM NaCl, 10 mM Tris, 1 mM CaCl2, 21 mM MgCl2) Generation of gel Beads-in-emulsion, cDNA amplification, and library preparation were performed with Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (10x Genomics) following the manufacturer's instructions. 8,000-10,000 nuclei were loaded per channel of a 10x Genomics chip.