GSM7473777: w1_noHS_control_rep1_LongRNA; Drosophila melanogaster; RNA-Seq
Total RNA was extracted from 2-4 day old female ovaries using the MirVana kit (Ambion). For Long RNA-seq, rRNA was depleted using antisense rRNA oligo hybridization followed by RNase H digestion, RNA Fragmentation, Reverse Transcription, dUTP incorporation, end Repair, size selection using Ampure XP beads, A-tailing, Adaptor ligation, UDG treatment and PCR amplification. For Small RNA-seq, small RNAs 18-29 nucleotide long were isolated using polyacrylamide gel purification followed by 2S rRNA depletion, 3' adaptor ligation, 5' adaptor ligation, reverse transcription, and PCR amplification.