GMNL-143 sequencing by NGS
Library preparation was followed the protocol of illumina TruSeq Nano DNA Sample Preparation Kit. 200 ng of genomic DNA was sonicated to around 550 bp. Fragmented DNA was processed to covert the overhangs into blunt ends and selecting library size with beads. After ends repairing, single A nucleotide was added to the 3 ends of blunt fragments. The corresponding single T nucleotide on the adapter with index was ligated to the fragments. The size of library was checked by electrophoresis, and the quantification was validated with Nanophotometer. All validated libraries were mixed, denatured and dilute to proper concentration for pair-end 250 cycles sequencing on MiSeq.