GSM5750917: 89-1Mo; Homo sapiens; miRNA-Seq
Samples were spun for 20 min at 4 °C at 800 rpm to 130 separate lipid, skim, and cellular fractions. For each sample, 50 μl of the lipid fraction was used for RNA extraction. RNA purification was performed using a 134 Norgen Circulating and Exosomal RNA Purification Kit (Norgen Biotech; Ontario, Canada) Illumina TruSeq Small RNA Prep protocol