GSM7977793: PBMCs, M3; Homo sapiens; RNA-Seq
Within two hours of collection, three blood samples were subject to timely PBMC separation using FICOLL PAQUE PLUS (STEMCELL Technologies, Vancouver, Canada). These samples were divided into two groups: experimental (MXF 1-3) and control (Control 1-3). Single-cell suspensions, loaded onto a 10X Genomics Chromium instrument, were used to capture 8000 single cells following the manufacturer's instructions for the 10X Genomics Chromium Single-Cell 3' kit V3 (10x Genomics, Pleasanton, CA 94588, USA). Subsequent cDNA amplification and library construction steps were performed as per the standard protocol. Libraries were sequenced on an Illumina NovaSeq 6000 sequencing system (paired-end multiplexing run, 150bp) by LC-Bio Technology Co., Ltd, (Hangzhou, China) at a minimum depth of 20,000 reads per cell.