GSM5751628: 85-PBMC; Homo sapiens; RNA-Seq
T cells from decidua basalis and matched PBMCs were enriched using the Dynabeads® Untouched™ Human T Cells Kit (Invitrogen).    T cell enriched samples were then labeled with Zombie NIR™ Fixable Viability Kit (BioLegend). Subsequently, cells treated with TruStain FcX™ (BioLegend), then labeled with fluorochrome- and TotalSeq™-C oligonucleotide-conjugated antibodies. Samples were then sorted into PBS (Ca++/MG++ free, 1% non-acetylated BSA, 1 mL/mL RNase inhibitor) using the BD FACSAria in a 3 laser (488nm, 561nm, 405nm) configuration, collecting 100,000 live, CD3+ cells. Sorted cells were then processed using the Chromium Next GEM Chip A Single Cell A Chip Kit (10X Genomics). According to manufacturer's protocol (10X Genomics Chromium, Single Cell Immune Profiling 5' v1.1 chemistry;  10X Genomics V(D)J Single-Cell RNA-Seq pipeline)