Illumina reads of DZ
A. alternata DZ was isolated from an infected common tobacco, Nicotiana tabacum, leaf from Hunan, China in 2020. The pathogenicity was validated by inoculating spores of the strain DZ on healthy leaves of Nicotiana tabacum and identified as Alternaria spp. based on morphology and ribosomal RNA internal transcribed spacer sequence. The fungus was grown on potato dextrose broth (PDB) medium (extract of 200 g of potato and 20 g of dextrose per liter) on a rotary shaker set at 160 rpm at 25C for 3 days. Genomic DNA of the A. alternata DZ was extracted from the mycelia using a Fungal DNA Kit (Tiangen Biotech Co., Ltd., Beijing, China), according to the manufacturers instructions. Illumina HiSeq 4000 and Oxford Nanopore technologies were used to generate 7.81 Gb of paired-end short sequence reads and 10.59 Gb of long reads, respectively, which is carried out in GrandOmic (Wuhan, China).