GSM7119471: HeLa_polyA_ModSeq_GS_MALAT1_Mn; Homo sapiens; OTHER
Trizol cDNA was prepared from polyA-enriched RNA from each source using gene specific primers, along with MarathonRT enzyme in the presence of either Mg or Mn as enzymatic cofactors; gene-specific PCR was done to get targeted amplicons from each cDNA sample (target 1 = MALAT1 lncRNA, target 2 = PRUNE1 mRNA); library preparation was carried out from resulting dsDNA amplicons using the Nextera XT DNA kit