Whole genome sequencing of a Trichophyton interdigitale isolated from a nail
Dermatophytes were grown in Sabouraud liquid medium. After growth, preliminary lysis steps are applied. Genomic DNA was then purified using the Qiagen DNEasy UltraClean Microbial Kit. DNA library construction was performed using Illumina's PCR-free DNA tagmentation kit (Illumina) according to the manufacturer's guidelines. Quality control of the DNA libraries was performed on Qiaxcel (QIAgen) while quantification was performed by qPCR (KAPA SYBR fast). Pooled libraries were sequenced on a Novaseq 6000 system (Illumina) using a NovaSeq S4 flowcell (151 cycles read 1, 10 cycles Index1, 10 cycles Index2, 151 cycles read 2). The expected sequencing coverage is approximately 50X.