GSM5751276: NR_GZ_023: Pedunculate & Olfactory lobe - smallRNA; Octopus vulgaris; ncRNA-Seq
Adult specimens of O. vulgaris (body weight 800 g ± 50 g, mean ± SD) were collected from the Bay of Naples (Italy) and transferred to the Department of Biology, University of Naples Federico II (Italy). Samples (N=8) were anesthetized by isoflurane insufflation (Polese et al. 2014), and tissues were dissected under sterile conditions following institutional guidelines. The collected samples were snap frozen in liquid nitrogen and immediately put in Trizol, then stored at −80 °C. The RNA has been extracted from the tissues using Direct-zolTM RNA Miniprep (Zymo Research), following the manufacturer's protocol. RNA has been stored at -80 C prior to sequencing, and it was transported using dry ice. The quality of RNA has been accessed with Bioanalyzer and only samples with intact RNA have been kept for library preparation. Library construction protocols are listed in the columns of SAMPLES section above