GSM5603820: NPC sc-seq WT-2; Mus musculus; RNA-Seq
Perfusion collagenase and pronase (0.4mg/ml) from portal vein into mouse liver was performed after euthanasia. Hepatocyte was removed from total cell suspension by gradient centrifugation. The NPCs were suspended in 1ml 2% FBS DMEM at a density of 106 cell/ml. RNA libraries were prepared for sequencing using standard Illumina and 10X genomics Chromium single cell protocols