GSM6729181: P15; Mus musculus; RNA-Seq
Animals were euthanized and spleen and marrow were harvested as viable single cell suspensions.  Dead cell depletion was performed using the Miltenyi dead cell removal kit (130-090-101). Single cells were isolated, barcoded cDNA was generated and libraries were constructed using the 10X Genomics 3' Capture kit v3 according to the manufacturer's instructions.