GSM7120095: S15 hiPSC-macrophages + lipofectamine control; Homo sapiens; RNA-Seq
RNA extraction of samples stored in DNA/RNA shield were performed as described previously [9]. In brief, samples were thawed at RT and resuspended in an equal volume of lysis buffer (Zymo Research) before starting the Quick-DNA/RNA miniprep (Zymo Research) protocol following the manufacturer's instructions. After a TURBOTMDNase (Thermo Fisher Scientific) treatment step, purity of RNA samples was checked using a NanoDropTM2000 spectrophotometer (Thermo Scientific) RNA quality was verified using the TapestationTM (Agilent, RNA screentape analysis). RNA sequencing was performed as described in Bartholomeus et al. [33]. In brief, RNA samples were prepared with the QuantSeq 30 mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen GmbH) following the standard protocol for long fragments. Obtained cDNA libraries were equimolarly pooled, up to 40 samples for one NextSeq 500 sequencing run (high output v2 kit, 150 cycles, single read, Illumina).