GSM7712395: PBS treated hepatocytes, replicate 2; Mus musculus; Bisulfite-Seq
Genomic DNA extraction by Maxwell® 16 Cell LEV DNA Purification Kit and biisufite conversion by EpiTect Bisulfite Kit, following provider indications. Finally, the promoter region of Arid1b and Shroom1 was amplified by PCR. Libraries were prepared using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina following the manufacturer's recommendations