ONT fast5 Sequencing of Ornithoptera alexandrae: larva female FC561 specimen
Tissues from the thorax or abdomen were used to extract high molecular weight DNA with the Qiagen genomic DNA kit. Whole-genome libraries were constructed using the resulting high-molecular-weight DNA as input for the Nanopore LSK-109 ligation kit (Oxford Nanopore Technologies, UK) following the manufacturers protocol. Long-read sequencing was performed on a GridION device with four R9.4.1 flow cells.