GSM7713000: Sylvio_MNase_35U_Replicate2; Trypanosoma cruzi; MNase-Seq
Late exponentially growing epimastigotes of the  Sylvio-X10 and CL Brener strains were collected and permeabilized as previously described with modifications (Leandro de Jesus et al., 2017).  Chromatin was digested with different amounts of MNase (units given in filenames) The DNA was repaired using the DNA PreCR kit from New England Biolabs. Paired-end libraries were constructed and indexed using Illumina kits from NEB (E7370 and E7335). Agencourt AMPure XP beads (Beckman, A63880) were used to purify the DNA after adaptor ligation and PCR products before sequencing