RNA-seq of Diastylis cornuta: whole body
Total RNA was extracted using TRIzol (Invitrogen, Waltham, MA) according to the manufacturer's instructions. Following extraction, total RNA was cleaned using the Nucleospin RNA Clean-up (Macherey-Nagel, Duren, Germany) silica-based column to further purify the RNA. Ribosomal RNA was removed using Ribo-zero (Illumina, San Diego, CA) and the quality of the RNA was checked on a Bioanalyzer. The organism was sequenced on an Illumina HiSeq 2500 with 125bp paired-end reads at Duke University.