DNA metabarcoding of a bacterial mock community: profiling with 799F/1193R primer sets without blocking (rep 1)
A mock community was assembled by extracting and mixing DNA from 17 bacterial species. PCR amplifications were then carried out to amplify a bacterial or fungal marker sequence in a metabarcoding approach. For some samples, a developed PCR clamp was added in the mix, with the aim of blocking host (wheat) DNA co-amplification (for this mock sample, the idea was to check the absence of inhibition of the developed clamps toward bacterial DNA amplification). Amplicons were sequenced on a 2x300 bp MiSeq Illumina device.