GSM6685265: hapln1a+, regeneration, scRNAseq; Danio rerio; RNA-Seq
Adult hearts were extracted at 7 days post amputation and the wounded apex region was collected and digested with Liberase TM (0.26 U/ml ). Dissociated cells were spun down and live EGFP+ cells were sorted by flow cytometry. Library was performed according to the manufacter's instructions (single cell 3' v3.1 protocol, 10x Genomics )