16S amplicons of lichen bacteriome: Antarctica
The obtained products were pooled in equimolar ratio within a particular lichen species (and site for generalist lichens  see Table 1) and indexed using the aforementioned Nextera XT barcodes (Illumina, San Diego, USA). Amplicon libraries were pooled and sequenced on the Illumina MiSeq instrument (Illumina, San Diego, USA) at the DNA Sequencing and Synthesis Facility (Institute of Biochemistry and Biophysics, Polish Academy of Sciences), with the sequencing conducted in paired-end mode (2  300 bp) with the use of a v3 (600 cycles) chemistry cartridge which allowed generation of long paired reads fully covering 16S V3V4 amplicons.