16S v3-v4 DNA-seq of microbiome from soil sample mixed seeds, Central district, Plovdiv, 2022
Total genome DNA from soil samples was extracted using CTAB/SDS method. 16S rRNA (V3-V4) was amplified used specific primer (341F/ 806R) with the barcode. All PCR reactions were carried out with Phusion High-Fidelity PCR Master Mix (New England Biolabs). The libraries generated with NEBNext UltraTM DNA Library Prep Kit for Illumina and quantified via Qubit and Q-PCR, were analysed by Illumina NovaSeq 6000 platform.