GSM8083647: CD8+ CAR-T cell, Blimp-1 KO, Donor 30; Homo sapiens; RNA-Seq
CD8+ cells were purified following the 4 co-cultures of Mock or Blimp-1 KO CAR-T cells and ARP-1-GFP-ffLuc cells using an EasySep Human CD8 Positive Selection kit (STEMCELL Technologies). Cell pellets were frozen and stored at -80ºC and isolation efficiency was confirmed by flow cytometry. Total RNA was extracted from the cell pellets using an RNeasy Mini kit (Qiagen) and subject to paired-end mRNA sequencing using the NovaSeq 6000 platform (Illumina) at a minimum of 30 million reads per sample. Libraries were prepared according to Illumina instructions.