GSM7977992: r22_r38_CD45+_salivary glands_scRNAseq; Mus musculus; RNA-Seq
For isolation of CD45+ cells in the salivary glands, tissue was cut into small pieces, digested by 2 mg/ml collagenase type II (Worthington Biochemical Corp) and homogenized. The suspension was purified by centrifugation using Lympholyte-M solution (Cedarlane) according to the manufacturer's protocol. Totalseq-Hashtag antibody (BioLegend) was incubated with each sample, and CD45+ cells were sorted by FACSAriaIII (Becton Dickinson). Libraries were prepared according to the protocol of the Chromuim Next GEM Single Cell 5' Reagent Kits v2 (10x Genomics) using a Chromium controller (10x Genomics).