GSM6619679: Embryo, E15.5, single nuclei ATAC; Mus musculus; ATAC-seq
We prepared single nuclei suspensions from fresh frozen embryos (E12.5, E13.5 and E15.5) that were littermates to those used for spatial ATAC. Three to five 70 µm frozen sections were obtained for each embryonic stage matching the anatomical landmarks from spatial ATAC sections. Frozen sections were then dissociated according to the 10X Chromium Single Nuclei Isolation kit (1000494) omitting the debris removal step to avoid cell loss. Nuclei suspensions were stained with 7-AAD (Miltenyi; 1:50) and sorted on a BD Fusion flow cytometer with a 100 µm nozzle. Nuclei were then immediately processed according to the 10X Genomics' Single Cell ATAC Next GEM kit v1.1