RNA-Seq of Helix lutescens
RNA sequencing library was prepared with the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA). mRNAs were enriched with Oligo(dT) beads, fragmented, transcribed to cDNA and enriched by limited-cycle PCR. The library was sequenced on the NovaSeq 6000 platform (2x 150 bp). We run rCorrector v1.0.4 (Song & Florea, 2015) to correct likely erroneous rare k-mers, and removed reads flagged with unfixable_error flag. Then we performed quality and adapter filtering with fastp 0.23.1 (Chen et al., 2018) under default settings except for --detect_adapter_for_pe.