linked-read genomic DNA sequencing of Nasonia oneida: adult male whole-body
A total of 0.7 ng high molecular weight DNA was used for library preparation using the 10x Genomics Chromium Genome Reagent Kit. The diluted denatured gDNA, sample master mix, and gel beads were loaded to the genomic chip following the protocol and then ran on 10x Chromium Controller to generate Gel Bead-In-EMulsions (GEMs). The obtained GEMs were used for the subsequent incubation and cleanup. Chromium i7 Sample Index was served as the library barcode. After quality control with Qubit 3.0 Fluorometer and Agilent TapeStation 4200, sequencing was performed on an Illumina HiSeq X Ten system.