RNA polymerase ChIP-seq of E. coli in M9 medium with lysine, replicate 1
RNA polymerase ChIP-seq samples, following IPOD-HR method. After cross-linking, cell lysis and DNase digestion, a fraction of the samples was collected to perform RNA polymerase ChIP-seq using antibodies against E. coli RNA polymerase beta subunit. After ChIP,cross-links were reversed, DNA purified and libraries prepared using the NEBNext Ultra II DNA kit.