GSM6767512: siTAZ3; Homo sapiens; RNA-Seq
Cells were transfected with siRNA using Lipofectamine® RNAiMAX Reagent (Thermofisher). Cells were maintained in DMEM supplemented with 10% fetal bovine serum (FBS) and antibiotics, and the medium was refleshed every three days. RNA was harvested using Trizol (Invitrogen). 1 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.