GSM7089344: HeLa, CTRL; Homo sapiens; RNA-Seq
Total RNA was isolated with TRIzol (Invitrogen cat: 15596026) and assessed for integrity using 2100 Agilent Bioanalyzer (Agilent, USA) before library preparation. The library preparation started with 1 μg of total RNA for each sample. For mRNA sequencing library construction, we first purified mRNA with poly-T oligo-attached magnetic beads and synthesized first-strand and second-strand cDNA. Rolling circle amplification (RCA) was performed to generate DNA nanoballs (DNBs).