GSM7712378: PBS treated hepatocytes, replicate 3; Mus musculus; Bisulfite-Seq
Genomic DNA extraction by Maxwell® 16 Cell LEV DNA Purification Kit and bisulfite conversion by EpiTect Bisulfite Kit, following provider indications. Finally, the promoter region of Pcsk9 was amplified by PCR. Libraries were prepared using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina following the manufacturer's recommendations