cortex70-52wks
Genomic DNA was isolated by direct lysis, followed by a two step PCR for targeted amplicon sequencing. In the first PCR amplicon specific primers were used, followed by bead-cleanup of the amplicon. In the second PCR, unique indices were annealed to all amplicons. Samples were pooled at equimolar ratios and gel-purified. Concentrations were determined using a Qubit fluorometer.