231_48hours
The total DNA was extracted using QIAamp DNA Micro Kit (Qiagen #56304). Then the DNA which was fragmented by using Covaris M220 Focused-ultrasonicator (Covaris) were subjected to sequencing library construction. Exome capture was performed using SureSelect Human All Exon V6 Kit (Agilent Technologies) following the vendor's recommended protocol. The sequencing was performed using the Illumina Novaseq 6000 (LC-Bio Technology CO., Ltd., Hangzhou, China) with 150-bp paired-end sequencing mode.