GSM8083424: PC9-YAP-S94A-rep2; Homo sapiens; RNA-Seq
RNA was extracted from snap-frozen tissue or cell pellets. For tissue samples, tissue was minced using a liquid nitrogen-cooled mortar and pestle before RNA extraction. RNA isolation was performed using the RNeasy Mini kit (Qiagen) including an on-column DNase I digestion. RNA quality was assessed by automated electrophoresis using the RNA 6000 Pico Kit and an Agilent 2100 BioAnalyzer (Agilent Technologies, Inc.). RNA was quantified using the Qubit RNA HS Assay Kit and a Qubit 2.0 fluorometer (Thermo Fisher Scientific). Library preparation and paired-end 150bp (PE150, Illumina) RNA sequencing was performed by Novogene (Novogene Corporation, Sacramento, USA).