GSM6619999: wing-disc, pellet, HWT, rep1; Drosophila melanogaster; OTHER
We performed CUT&RUN in wing disc tissue as described in K. Ahmad (2018) modified from P. J. Skene, et al. (2018). For each replicate, twenty WL3 imaginal wing discs along with two wing discs of D.yakuba were used per replicate. For the H3K27me3 experiment, aH3K27(CST#9733,1:100) and protein AG-MNase (1:100, UNC core) were used. For the H3.3 experiment, aH3.3 (H3F33B, Abnova #H00003021-M01, 1:100) and protein A-MNase from the Henikoff lab were used. The Thruplex DNA-seq kit was used for the library preparation. The manufacturer's protocol was followed until the amplification step. After the addition of indexes, 16-21 cycles of 98C, 20s; 67C, 10s were run. DNA library purification was done using AMPure XP beads. Libraries were sequenced on an Illumina NextSeqP2. 