GSM6729376: shCtrl-2_THP-1_RNA-seq; Homo sapiens; RNA-Seq
After infection by  shCtrl and shSMARCA5 lenti-virus for 48h, Kasumi-1 and THP-1 cells were selected by puromycine for 48h and then harvested for RNA extraction with TRIzol Reagent (Invitrogen). (RNA-seq) After infection by  shCtrl and shSMARCA5 lenti-virus for 48h, Kasumi-1 and THP-1 cells were selected by puromycine for 48h and then harvested for gDNA extraction. (ATAC-seq) Libraries were generated using the TruSeq RNA Sample Preparation Kit (Illumina #RS-122-2001) and sequenced with Illumina NovaSeq6000.(RNA-seq) Libraries were generated using the Chromatin Profile Kit for Illumina (Novoprotein #N248-01A) and sequenced with Illumina NovaSeq6000.(ATAC-seq) Library preparation was according to the manufacture's guidelines (NovoNGS CUT&Tag 3.0 High-Sensitivity Kit for Illumina) and sequencing procedures were carried out according to Illumina protocols with minor modifications (Illumina, San Diego).(CUT&Tag)