GSM7977500: heart tissue, GPX genotype, HFHS diet, rep 4; Mus musculus; RNA-Seq
Total RNA was extracted from left ventricular tissues (~10 mg), using the RNeasy Fibrous Tissue Kit (QIAGEN, Germantown, Maryland) Illumina TruSeq® Stranded mRNA Library Prep kits (Illumina) were used to prepare sequencing libraries from mRNA, followed by reverse transcription to cDNA, fragment purification and ligation to indexed (barcoded) adaptors. Indexed libraries were pooled for multiplex sequencing and run on an SP flow cell on the Illumina NovaSeq 6000 sequencer for 300 cycles with 150-base paired-end reads.