Hi-C DNA Seq. of Tripidium ravennae: leaf tissue
Leaf tissues were fixed with formaldehyde, lysed, and the crosslinked DNA digested with Sau3AI. Sticky ends were biotinylated and proximity-ligated to form chimeric junctions, that were enriched for and then physically sheared to a size of 300-500 bp. Chimeric fragments representing the original crosslinked long-distance physical interactions were then processed into paired-end sequencing libraries and paired-end Illumina reads were produced.