GSM8084320: KPC tumor from a Pdgfrb-TK mouse treated with PBS; Mus musculus; RNA-Seq
PVN ncueli was obtained from compiling 5 micorpunched PVNs together and then carrying out nuceli isolation using a sucrose gradient. The nuceli morpholohy and quantity were checked under the microscope by staining Single cell Gene Expression Library was prepared according toChromium Next GEM Single Cell 3' Reagent Kits v3(10x Genomics). In Brief, single nuclei suspension, reverse transcription (RT) reagents, Gel Beads containing barcoded oligonucleotides, and oil were loaded on a Chromium controller (10x Genomics) to generate single cell GEMS (Gel Beads-In-Emulsions) where full length cDNA was synthesized and barcoded for each single cell. Subsequently the GEMS are broken and cDNA from each single cell are pooled. Following cleanup using Dynabeads MyOne Silane Beads, cDNA is amplified by PCR. The amplified product is fragmented to optimal size before end-repair, A-tailing, and adaptor ligation. Final library was generated by amplification.