GSM6771552: CFP1 ChIP seq BL6-WT-PD4-input; Mus musculus; ChIP-Seq
Chip analysis was performed with a slight modification of the manufacturer's instructions using the ChIP-IT Express Enzymatic kit (Active Motif, Carlsbad, CA, USA). Briefly, Cfp1f/f and Cfp1d/d mouse uterus horns on Day 4 were cut vertically, and then separated by epithelial cells and stromal cells except smooth muscles using a scalpel and chopping small. Afterward, cells were fixed with 1% formaldehyde, DNA fragments between 150 and 500 base pairs (bp) were obtained by enzymatic shearing cocktail. The construction of the library was performed using NEBNext® UltraTM DNA Library Prep Kit for Illumina (New England Biolabs, UK) according to the manufacturer's instructions. Briefly, the chipped DNA was ligated with adaptors. The library was purified by using magnetic beads to remove all reaction components.