GSM7049747: Input_NI_3; Homo sapiens; RIP-Seq
LoVo cells were grown to 70–85% confluence in 75 cm2 flasks (2 per condition) and infected or not with Lm LL195 as described above. Flasks were snap-frozen in liquid nitrogen at 10 h p.i., and then stored at −80°C until further use.  RNA-immunoprecipitation was performed as described in Kwon et al., 2021, Nature Comm. 10% of the lysate was taken as Control Inputs, and acid phenol was used to extract total mRNA from the input fractions and RNA from the immuno-precipitated fractions, either with an anti-CIRBP antibody or a control anti IgG antibody. Purified RNA duplicates were pooled after the DNAse step and phenol/chloroform precipitation for every condition. Purified RNAs were stored at −80°C and further used in sequencing library construction. Each time course was reproduced at a 1-week interval, thus producing biological triplicates. Library were built following the Illumina Stranded mRNA ligation protocol and then sequenced on an Illumina NextSeq2000 sequencer and  with 2 P2 flowcells.  SR100 reads were generated.