E_oocyte
Extracted RNA was used for cDNA synthesis and mRNA library preparation using SMART-Seq v4 Ultra Low Input RNA Kit (#634894 , Takara Bio) and Nextra XT DNA library preparation Kit (#FC-131-1024 , illumine, San Diego, CA, USA). RNA sequencing was conducted on the illumine NovaSeq 6000 (illumina, San Diego, CA, USA), generating on average 10.9Gb of raw 150 bp paired-end reads per sample