GSM6619985: CR013_Lund_KAP1KO_H3K9me3; Homo sapiens; OTHER
CUT&RUN was done on 250,000 cells per condition following the protocol of Henikoff and colleagues (Skene et al, 2018). DNA was purified by the Nucleospin PCR clean-up kit (Macherey-Nagel). Antibodies were: rabbit anti-H3K9me3 (ab8898, 1:100 dilution) and guinea pig anti rabbit IgG (CSB-PA00150E1Gp, 1:100 dilution) Illumina sequencing libraries were prepared using the Hyperprep kit (KAPA) with unique dual-indexed adapters (KAPA) following the manufacturer's instructions