Arachis hypogaea GBS RIL population
Genotyping-by-sequencing (GBS) was performed according to the protocol reported by Elshire et al., 2011. For GBS, 10 ng of DNA from each line was digested using the restriction endonuclease ApeKI. This enzyme recognizes the site- G/CWCG, followed by the ligation of barcode adapters to digested products. An equal proportion of adapter-ligated fragments were used for library construction. These libraries were filtered by amplifying to remove additional adapters and sequenced on HiSeq 2500 (Illumina Inc, San Diego, CA, USA)