GSM7926293: CPM,IgG,CUT&Tag; Gallus gallus; OTHER
We utilized the Hyperactive Universal CUT&Tag Assay Kit for Illumina Pro (TD904-1) from Vazyme. The Anti-MEF2A Rabbit pAb (GB11965) from Servicebio was chosen to detect the MEF2A-bound DNA fragments. By overexpressing MEF2A in primary myoblasts and harvesting them with ConA beads, the sufficient amount and purity of starting material for the CUT&Tag assay were prepared. Subsequently, the cells were incubated with the primary antibody overnight, followed by a secondary antibody and pA-Tn5 transposase, which allowed for precise fragmentation and tagging of DNA segments. Library construction was performed using the TruePrep Index Kit V2 for Illumina (TD202) from Vazyme.