GSM7384526: H3K27ac_HiChIP_mESC_JMJD2C_Mut_hIDR1_R2; Mus musculus; OTHER
In-situ Hi-C was performed as described earlier (Rao et al., 2014). Briefly, 15 million ESCs were crosslinked and digested with MboI (NEB, R0148). After digestion, biotin was incorporated into the sticky ends of fragments before ligation. H3K27ac ChIP was performed to enrich for proximity ligations bound to H3K27ac (Mumbach et al., 2016), using H3K27ac antibody (Abcam, ab4729). The quality of libraries was checked by Agilent High Sensitivity DNA kit (Agilent Technologies) before sequencing.