GSM7089839: U2OS-d21 rep1; Homo sapiens; OTHER
Genomic DNAs were isolated from the cell pellets using the Blood & Cell Culture DNA Maxi Kit (Qiagen) according to the manufacturer's instructions. sgRNA sequences were amplified from 200 μg of gDNA by 20 parallel PCR reactions (10 μg of gDNA/ tube) using Q5 High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA). The resulting amplicons were mixed and purified by gel extraction using Wizard SV Gel and PCR Clean-Up System (Promega).