epiGBS of Biomphalaria glabrata
We applied an existing protocol of epinogenotyping by bisulfite sequencing (epiGBS) library preparation (Gawehns et al., 2022) to the snails characterized by significantly lower prevalence of infestation than controls. We modified some steps of the existing protocol. Instead of pooling all samples, we separated samples after the barcoding step. We also modified the bisulfite conversion step following the in-house protocol described in previous work (Luviano et al., 2021). Eight individuals of the epiRIL 8-7, eight of the epiRIL 9-3 and eight controls were sequenced. Paired-end sequencing (2 150 bp) was performed using Illumina Novaseq system at Fasteris (www.fasteris.com).