Illumina HiSeq 2500 sequencing: age dependent changes in Caenorhabditis elegans gut microbiome
Worms were raised on live E. coli until the L4 stage to ensure proper development, then transferred to kanamycin-killed E. coli from which worms were further transferred at advancing ages to microcosm environments for two days before harvesting for analysis harvesting for analysis. DNA was extracted using QIAGEN PowerSoil DNA isolation kit and 16S V4 region was sequenced.