soil microbiome of AMF
The products of the second PCR were assessed via gel electrophoresis, then normalised using Sequalprep normalisation plates (Life Technologies, Tokyo, Japan). Equal volumes of each sample were pooled into a library and cleaned using Ampure ex beads (Beckman Coulter, Tokyo, Japan). Library molarity was determined via Qubit fluorometry (Life Technologies, Singapore), and quality checked with Tapestation sizing. Amplicons were sequenced using Illumina MiSeq 2x300bp V3 600 cycle sequencing kit (San Diego, CA, USA)