RNA-seq of Homo sapiens: human cerebellar astrocytes treated with IL1b and apigenin
RNA quality was assayed using Agilent Technologies 2200 TapeStation and only samples with high RNA quality (RIN > 8) were used for library preparation. Stranded mRNA-Seq libraries were prepared using the Illumina TruSeq Stranded mRNA Library Prep Kit according to the manufacturers instructions and single-end reads were acquired on an Illumina HiSeq 2500 or 4000 instrument.