GSM8084081: RNA-Seq_Embryo cortex_E18.5 HFD rep 2; Rattus norvegicus; RNA-Seq
Total RNA was isolated from E14.5, E16.5, and E18.5 rat embryo cortex using the MasterPure™ RNA Purification Kit from Epicentre (Cat. #MCR 85102) following the manufacturer's guidelines. RNA libraries for sequencing were then prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7775) according to the provided instructions. During library preparation, 1 µg of total RNA was depleted of ribosomal RNAs using NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat) (E7400L) and purified with AMPure XP beads.