GSM6809596: Ductal_KC; Mus musculus; RNA-Seq
FACS purified KC ductal cells acquired on a BD FACS ARIA (BD Biosciences) and sorted cells collected in Dulbecco's Modified Eagle Medium (DMEM) containing 20% FCS. Cell encapsulation, barcoding, and library preparation followed the standard scRNAseq protocol comprising reverse transcription, amplification, and indexing (10xGenomics). Sequencing was performed using a NovaSeq Illumina device (Illumina).