GSM7926136: EE, H3K27me3,  rep1; Mus musculus; OTHER
Proximal mouse intestine was harvested and epithelial cells were eluted with 20 mM EDTA + 1 mM DTT and filtered through 100 and 40 um cell strainers. Filtered cells were sorted on Sony MA900 and XDP100 machines for cells positive for reporters/antibodies. Nuclei were isolated following Heinekoff et al. "Benchtop CUT&Tag". CUT&Tag was performed as in Heinekoff et al. "Benchtop CUT&Tag" for each antibody. Libraries were amplified using 13 cycles on the thermocycler. Post amplification libraries were purified using Agencourt AMPure XP beads from Beckman Coulter. Libraries were validated using Agilent High Sensitivity DNA kit.