DNAseq of swine slaughterhouse clean zone after cleaning and disinfection
1. Precise quantification of genomic concentrations using the Qubit dsDNA HS Assay Kit to determine the addition of library constructionTotal amount of DNA.2. A library of about 500 bp inserted fragment length was prepared, and the initial total DNA was about 500 ng. Use the ElutionBuffer diluted DNA to 130  L and loaded into 0.5 mL Covaris DNA fragmentation tube.3. Fragment according to S220 operating parameters using Covaris instrument.4. Concentrate and recycle broken DNA fragments using 1 times Hieff NGS DNA Selection Beads. purificationQubit 4.28 was used for detection.