GSM8083219: Molar, Notum KO homozygous, E16.5, biol rep2; Mus musculus; RNA-Seq
Maxillary and mandibular molar tooth germs were dissected from Notum+/+and Notum-/- littermate mouse embryos at E14.5 and E16.5. Total RNA was extracted with TRIZOL® (Invitrogen) RNA extraction method. The RNA quality was assessed using RNA ScreenTape® (Agilent Technologies, Germany). RNA Library Preparation and Sequencing was performed by Novogene, China. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis. The library was prepared, followed by the end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries were pooled and sequenced on Illumina platform.