GSM6813046: parental_INH2 [mIDH1INH2 ]; Homo sapiens; RNA-Seq
SF10602 cells were collected and suspended in digestion buffer. Digestion was performed using microccocal nuclease to obtain 150 pb DNA fragments. Histone-DNA complexes were isolated with the desired antibody. Libraries for ChIP-seq were prepared using the TruSeq ChIP Sample Preparation Kit. Input and IP DNA was blunt-ended and phosphorylated, followed by the addition of a single adenine nucleotide prior to ligation with an adapter duplex with a T overhang. Ligated products were size selected by agarose gel electrophoresis, purified and PCR amplified for the final library preparation.