GSM7474144: lib64235; Homo sapiens; RNA-Seq
Unfractionated colon biopsies taken from IBD patients before and during treatment with vedolizumab were processed for RNAsequencing. SMARTseq v4 chemistry was used to generate cDNA following the manufacturer's instructions. Sequencing libraries were constructed using the NexteraXT DNA sample preparation kit with unique dual indexes (Illumina) to generate Illumina-compatible barcoded libraries. Libraries were pooled and quantified using a Qubit® Fluorometer (Life Technologies). Sequencing of pooled libraries was carried out on a HiSeq 2500 sequencer (Illumina) with paired-end 53-base reads and a target depth of 5 million reads per sample.