Long-read sequencing (PacBio HiFi) of Siderastrea siderea: whole adult polyp
DNA extractions were performed using Qiagen MagAttract HMW extraction kits on DNA/RNAshield-preserved tissues collected in Curacao. Following initial extraction, DNA was further purified using a 0.9X AMPure XP (Beckman Coulter, CA, USA) bead cleanup. Purified DNA was then size selected using a Pacific Biosciences (formerly Circulomics) SRE size selection kit. DNA was sent to the Penn State Huck Institutes for Life Sciences Genomics Core Facility for template preparation and PacBio HiFi sequencing (Sequel IIe). 3 samples were barcoded using the PacBio SMRTbell Express Template Kit and sequenced together on a Sequel IIe SMRTcell prior to being demultiplexed with PacBio Lima.