GSM7711535: Gastric organoid, DMSO, scRNAseq; Mus musculus; RNA-Seq
To prepare cells from organoids for scRNA-seq, we followed the protocol outlined in the PIPseq Milli 3' Single Cell Capture and Lysis User Guide (Fluent BioSciences). The resulting emulsions carrying captured mRNA were visually inspected by Fluent Biosciences upon sample receipt to ensure their quality before proceeding with downstream processing. Samples that passed inspection were processed according to the specifications of the PIPseq™ T2 Single Cell Sequencing Kit, including mRNA isolation, cDNA generation, cDNA amplification, sequencing-ready library preparation, library pooling, and sequencing