PacBio genome sequencing of Escherichia albertii type strain
Bacterial strain was optained by DSMZ. It was cultivated at 37 C in lysogeny broth, cells were harvested by centrifugation and gDNA was isolated following a phenol/chloroform extraction protocol. RNase digestion was performed.gDNA was solved in TE buffer. Libary preparation and sequencing was outsourced to the company GENEWIZ. There another RNase treatment was performed, following by an AMPure bead clean up, library preparation with the PacBio Library preparation, 10 kb, and the PacBio Sequel sequencing. Genome assembly was then performed by us with Canu 2.1.1. Quality and completness was then assessed by QUAST v5.0.2 and busco v5.2.2.