GSM7712968: HeLa cells, ORP9 KO, biol rep 1; Homo sapiens; RNA-Seq
RNA was harvested using Rneasy min plus kit (Qiagen). 1.0 ug of total RNA was used for the construction of sequencing libraries mRNA isolation was performed using Oligo(dT) beads, and the mRNA fragmentation was performed using divalent cations and high temperature. First strand cDNA and the second-strand cDNA were synthesized using random primers. The purified double-stranded cDNA was then treated to repair both ends and add a dA-tailing in one reaction, followed by a T-A ligation to add adaptors to both ends. Size selection of Adaptor-ligated DNA was then performed using DNA Clean Beads. Each sample was then amplified by PCR using P5 and P7 primers and the PCR products were validated