NextSeq 500 sequencing; 2-hydroxyglutarate modulates histone methylation using both RNA/ChIP-seq
2-hydroxyglutarate modulates histone methylation using both RNA/ChIP-seq
RNA: The cells were lysed with the addition of lysis buffer (1M Sorbitol/ 100mM EDTA) and zymolyase (Amsbio, 120493-1) to final concentration 100 units/mL, and incubated for 30 minutes at 30oC.  RNA: The RNeasy Mini Kit (Qiagen, 74106) was used to extract the RNA from the lysed cells according to manufacturers instructions for yeast cells. The RNA was further cleaned with the DNase Max Kit (Qiagen, 15200-50).  RNA samples library was generated by using the TruSeq Stranded mRNA Library Prep kit (Illumina) with 1 g of RNA as input according to the manufacturers instructions. The libraries were then adjusted to 4 nM.