Hippo_day16_con_S4_L002_R1_001.fastq.gz
RNA extractions were quantified using RNA Nano Chips (Cat. #5067-1511) on an Agilent 2100 BioAnalyzer.  RNA-Seq library preps were constructed using the Illumina TruSeq Stranded mRNA Library Prep kit (Cat #20020595) using 500ng of total RNA as input, amplified by 11 cycles of PCR.  Final libraries were visualized using High Sensitivity DNA ScreenTape (Agilent, Cat. #5067-5584) on the Agilent TapeStation 2200 instrument.  Quant-It (Invitrogen, Cat. P11495) was used for final concentration determination and libraries were pooled equimolar.  The pool was sequenced paired-end 50 cycles on an Illumina NovaSeq6000 SP 100 Cycle flowcell-v1.5 with 2% PhiX spike-in.