RNA-seq of mus musculus: young adult female spleen follicular zone
Total RNA was extracted from tumors using Trizol combined with Qiagen miRNEasy mini kit with additional on column DNAse I digestion. Following isolation of RNA, cDNA libraries were built using the Illumina TrueSeq RNA Sample Preparation kit V2 (RS-122-2001).  An Agilent Bioanalyzer was used to determine RNA quality (RIN >8) prior to sequencing.  RNA-Seq libraries were sequenced on an Illumina HiSeq 2000 (single-end 50bp).