GSM7838921: mTS, NGS; Mus musculus; OTHER
mTS were digested by Accutase and organoids were removed from Matrigel by Cell Recovery Solution, then DNAs were extracted from mTSCs or organoids for PCR amplification using the same primers for library validation. PCR products were treated with End Prep Enzyme Mix for end repairing, then index were added using PCR reaction. The final library product for sequencing were then purified by beads. The qualified libraries were sequenced pair end 150bp on illumina Novaseq System.