GSM7474584: D1_1_GFP+_GFP; Homo sapiens; RNA-Seq
Single cell cDNA was done following the the Chromium Single Cell V(D)J Reagent Kits User Guide (CG000086 Rev M; 10x Genomics). Resulting cDNA was used to do a target amplification of GFP cDNA barcoded transcripts. Transcripts were sequenced on the Illumina NovaSeq platform. GFP+ or GFP- T cells were extracted using Fluorescence Activated Cell Sorting (FACS).