GSM7473650: F42N: Brain, APOE2; Mus musculus; RNA-Seq
Hemibrains were removed, flash frozen on dry ice, and RNA was harvested using the miRNeasy mini kit. Subsequent validation was done for quality and quantity utilizing RNA 6000 Nano kit on the Agilent 2100 bioanalyzer. RNA-Seq libraries were prepared robotically on a Beckman Biomek Fxp liquid handler using the Illumina TruSeq Stranded mRNA Library Prep kit (catalog # 20020595) starting from 500 ng of total RNA with 10 cycles of final PCR amplification. All libraries were pooled equimolar and sequenced as Single-read 50 across three lanes of an Illumina HiSeq4000 flowcell.