16S rRNA V4 gene amplicon sequencing of mouse3_male_WT_treated_Day14_fecal pellet (515F/806R) [JMF-2112-06-0038A]
Amplicon sequencing of mouse3_male_WT_treated_Day14_fecal pellet. Timed matings were set up in the same animal room to ensure that all mice were of the same age. The offspring were genotyped and weaned at 3 weeks of age. At weaning, the offspring from different litters were mixed and 3 CDHR5-proficient and 3 CDHR5-deficient mice from different litters were caged together until they were 8 weeks old. Fecal pellets were collected from 8 week old adult mice. After pellet collection, adult females were remixed and 3 CDHR5-proficient and 3 CDHR5-deficient mice were housed together per cage. Adult male mice could not be mixed but were split to have 3 mice per cage (either 2 CDHR5-proficient and 1 CDHR5-deficient mice or 2 CDHR5-deficient and 1 CDHR5-proficient mice) to minimize cage effects. Mice were treated with DSS in sterile autoclaved drinking water (female mice 2.0%, male mice 1.5% DSS) for 7 days, followed by a 7-day recovery period without DSS. Control mice received sterile autoclaved drinking water throughout the experiment. The weight and condition of the mice were monitored daily to ensure colitis. A second collection of fecal pellets was performed under a hood on day 14 after the recovery period and the pellets were immediately frozen in a pre-weighed Eppendorf tube on dry ice. Sequencing was performed as described in https://doi.org/10.3389/fmicb.2021.669776. {primers: GTGYCAGCMGCCGCGGTAA + GGACTACNVGGGTWTCTAAT, nucleic acid: DNA, flow cell identifier: K7H6J, barcoding: UDB-H12, plate-position: 1 A6, barcodes: AGCGCATATCCA + AGCTCTAGAAAC}.