Illumina NovaSeq 6000 paired end sequencing; RNA-seq of WT vs isw1 delta S. cerevisiae cells during Unfolded Protein Response time course
RNA-seq of WT vs isw1 delta S. cerevisiae cells during Unfolded Protein Response time course
WT and isw1Δ exponentially growing cells were treated with 1 μg/mL tunicamycin for 2h, washed and resuspended in Tm free medium. Samples were collected at t= -2h (Untreated), t = 0 (Tm 2 H), and t = 3 H (Wash 3 H) for analysis by RNA seq RNA was isolated from 10 OD of exponentially growing cells washed in MilliQ water and snap frozen. RNA was extracted with the Nucleospin RNA II Kit (Macherey-Nagel) mRNA sequencing libraries were prepared from polyA+ RNAs purified from 1 ug of total RNA using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations. A 150 bp read sequencing was Illumina sequenced with multiplexing. A mean of 17.7 ± 2.4 million passing Illumina quality filter reads was obtained for each of the samples. Library preparation was performed by Novogene (Novogene Co., Ltd).