GSM6893696: parental HL60 cells [miRNA-seq]; Homo sapiens; miRNA-Seq
Total RNA was extracted from HL60 and R-HL60 cells using TRIzol Reagent (Thermo Fisher Scientific, Waltham, MA, USA) and according to the instruction manual. The RNA samples were submitted to Welgene Biotech. Co., Ltd. (Taipei, Taiwan, R.O.C) for small RNA sequencing analysis. RNA quantity was determined using an ND-1000 spectrophotometer (Nanodrop Technology, Wilmington, DE, USA) and RNA quality was verified using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). miRNA sequencing libraries were prepared using the QIAseq miRNA Library Kit (Qiagen, Hilden, Germany) according to the manufacturer's protocol, and were sequenced using an Illumina NGS system.