RNA-seq: Flp-In 293, 3d induced
RNA isolation was performed using the RNeasy Minikit (Qiagen, 74104), followed by 30 min. of DNase treatment (Ambion, AM2238) at 37C, poly(A)+ RNA transcripts were isolated [NEBNext poly(A) mRNA magnetic isolation module; New England Biolabs, E7490] from 1g of total RNA for RNA library preparation and sequencing using NEBNext Ultra Directional RNA Library Preparation Kit for Illumina (New England Biolabs, E7420S) according to the manufacturers instruction. The samples were sequenced on Illumina HiSeq 4000 (FLP-In 293) with 100-bp paired-end reads.