ITS2 amplicon sequencing of Symbiodiniaceae
Symbiodiniaceae genomic DNA was extracted from coral samples using the TOYOBO MagExtractor Genome extraction kit (TOYOBO, Osaka, Japan) according to the manufacturers instructions.Amplification of ITS2 were conducted using primer pair ITSinftfor2 (Lajeunesse and Trench, 2000) and ITS2-reverse (Coleman et al., 1994). Prior to high-throughput (HTS) amplicon sequencing, indexing, and sequencing adapters were added to the purified amplicons by PCR following the manufacturer s instructions. HTS sequencing was performed on the Illumina MiSeq platform (Illumina, San Diego, USA) using a paired-end MiSeq Reagent Kit v.2 (2  250 bp).