combined miRNA/mRNA-seq: P120 mouse hippocampus
Total RNA was isolated using the mirVana miRNA isolation kit. QIAseq miRNA library QC and ERCC spike in RNAs were added to 50 ng of RNA per sample. Libraries were generated using the NEXTFLEX Combo-Seq mRNA/miRNA kit (Perkin Elmer) and sequenced on the NovaSeq 6000 (Illumina).