GSM7712326: W22; Homo sapiens; RNA-Seq
For RNA sequencing, total RNA was extracted with TRI Reagent (Sigma, Cat# T9424). DNA was removed by using a RNeasy MinElute Cleanup Kit (QIAGEN) with RNase-Free DNase Set (QIAGEN). RNA-seq Libraries were prepared with a NEBNext Ultra II RNA library prep kit from Illumina (New England Biolabs Inc., Ipswich, MA) as described in the manufacturer's protocol. RNA was extracted with QIAGEN RNeasy RNA isolation kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyzer.