GSM6021908: polyp16d; Nematostella vectensis; RNA-Seq
Whole embryos, animals, or dissected tissues were treated with TrypLETM Select Enzyme (A1217702, Thermo Fisher Scientific). Enzyme concentration and incubation time were adapted to each stage. Cell viability and counts were assayed with a Cellometer X2 (Nexcelom). Cell suspensions were diluted and loaded into a 10x Genomics single cell platform using vs2 and vs3 reagents. 10X Genomics Chromium v2/v3 chemistry