GSM7712186: control RV male (CTRL-m_1); Rattus norvegicus; RNA-Seq
MCT tissues from MCT-treated rats were harvested after RHC measurements and snap-frozen. Total RNA from each batch of RV samples were isolated separately using the RNeasy Mini Kit (or miRNeasy Micro Kit, when less sample provided-Qiagen) and snap-frozen. Purification of total RNA was performed as described in the RNeasy handbook, using DNase I digestion (RNase-Free DNase Set, Qiagen). Following the RNA integrity measurement, approximately 2 µg of total RNA were used as starting material for library preparation using the VAHTS Stranded mRNA-seq Library preparation following manufacture's protocol (Vazyme)