GSM7118609: H295R_RIP_input_Rep2; Homo sapiens; RIP-Seq
Beads were resuspended in TRIZOL to release RNPs. RNA isolation was performed using ZYMO Research Direct-zol RNA Microprep kit with on-column DNaseI treatment. Ribosomal RNA was depleted from the samples as described in Baldwin et. al., 2021 (PMID: 34165268). Library was prepared using KAPA RNA Hyper kit and KAPA Dual index adapters.