Replicate 2 of phototrophic mixed culture grown in Phosphorus plus testing condition.
Phototrophic Mixed Culture enriched with PNSB were grown in a media containing fuel synthesis water, diluted 5x with the addition of 20 mL/L of trace elements solution and 1 mL/L of vitamins solution in accordance with ATCC medium 2672 and sodium bicarbonate as inorganic carbon source to a final concentration of 7.3 g/L. Potassium dihydrogen phosphate = 65 mg/L and Ammonium chloride = 90 mg/L was used as nitrogen source. Genomic DNA was extracted from bacterial samples using DNeasy PowerWater kit following the manufacturers protocols. Extracted DNA concentration was quantified by NanoDrop 2000/2000c and adjusted to 20 ng of DNA/L of sample and sent to Qatar Biomedical Research Institute for sequencing on a MiSeq system (Illumina, USA).