GSM7838625: BMDM,SIRT7-/-,control,rep3; Mus musculus; RNA-Seq
Total RNA of each sample was extracted using TRIzol Reagent/RNeasy Mini Kit (Qiagen)/other kits. Total RNA of each sample was quantified and qualified by Agilent 2100/2200 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA), NanoDrop (Thermo Fisher Scientific Inc.). 1 μg total RNA was used for following library preparation. Next generation sequencing library preparations were constructed according to the manufacturer's protocol. Poly(A) mRNA isolation or rRNA removal, mRNA fragmentation and priming, first and second-strand cDNA synthesis, end preparation, adaptor ligation, size selection (~400 bp fragments), PCR amplification, and cleanup. Size: ~400 bp fragments.