Illumina sequencing of Listeria phage LP-125 oat milk-evolved mutant 1
Digestion, extraction, and precipitation of phage DNA were completed as previously described by Song et al. (Homburgvirus LP-018 Has a Unique Ability to Infect Phage-Resistant Listeria monocytogenes. Viruses 11, 1166 (2019)). Nanodrop and Qubit readings were taken to determine the concentration and then samples were stored at -20C. Library preparation and sequencing were performed by SeqCenter (Pittsburgh, PA). Libraries were prepared using Illumina DNA library preparation kits and IDT 10bp UDI indices (Illumina) and sequenced with an Illumina NextSeq 2000, with 151-bp paired-end read chemistry.