GSM5603496: Zmroot_sc_read1/2_rep4; Zea mays; RNA-Seq
Root tips from maize seminal and principal roots were cut and collected (3-4 mm lenght). Root tip were incubated in a enzymatic solution to digest cell walls and produce protoplasts. Protoplasts were filtered using a cell strainer and used as input for the 10x Chromium Single Cell 3' V2 pipeline. Libraries were prepared using Chromium Single Cell 3' Reagent Kit V2 and V3