A6T0
The Swift amplicon 16S+ITS Panel Kit (Swift Biosciences, Inc., Ann Arbor, MI, USA) was used, following the manufacturers instructions, by applying 22 amplification cycles in the first PCR step and by introducing a purification step of the libraries with AMPure XP beads (0.8, v/v) (Beckman Coulter, Inc., Brea, CA, USA) at the end of the protocol. Each NGS library was quality checked through TapeStation 4200 (Agilent Technologies, Santa Clara, CA, USA) or 1.2% agarose gel electrophoresis and quantified using fluorometric assay (Qubit dsDNA HS assay kit, Thermo Fisher Scientific, Waltham, MA, USA).