Bisulfite cfDNA-Seq of Homo Sapiens: Vietnamese healthy control
Cell free DNA extraction was performed using plasma aliquots with the MagMAX Cell-Free DNA Isolation kit (ThermoFisher, USA), following the manufacturers protocol. Bisulfite conversion and purification of cell-free DNA samples were conducted using EZ DNA Methylation-Gold Kit (Zymo research, D5006, USA), following the manufacturers instructions. Library preparation from bisulfite-converted DNA were performed using xGen Methyl-Seq DNA Library Prep Kit (Integrated DNA Technologies, 10009824, USA). DNA libraries were sequenced on DNBSEQ-G400 DNA sequencing system (MGI Tech, China) to generate sequencing data with 100-bp paired-end reads, at a sequencing depth of 20 million reads.