GSM7179120: TCR.Spleen_Day30; Mus musculus; OTHER
We applied E294–302 tetramer selection to dissect virus-specific CD8+ T cells within different tissues of ZIKV infected Ifnar1-/- mice, including spleen, PBMCs, brain and testicle, at different onset times (7 and 30 dpi). We performed single TCR sequencing and single-cell RNA sequencing (scRNA-seq) with 10× Genomics platform. Moreover, we also sequenced TCRs of general CD8+ T cells from the spleen at 0 dpi. After quality control processing and removing non-T cells, 40,371 high-quality single T cells were retained for the downstream analysis.