GSM7711759: Huh7 Cells, Pla B (Pla B, + 4sU), REP2; Homo sapiens; miRNA-Seq
Total RNA was extracted using TRI reagent. 8μg of RNA were then used in an IAA-alkylation reaction, and re-precipitated before library precipitation. Libraries were prepared with the NEXTflex Small RNA-seq kit, according to manufacturers protocol. Libraries were pooled and size selected by excision from a 3% agarose gel.