GSM7893039: pcMSC-E2-CM; Homo sapiens; RNA-Seq
Total RNA from exosomes (CM and E2-CM) was extracted using the TRIzol LS reagent (Invitrogen, USA) according to the manufacturer's instructions. RNA quality was evaluated using the LabChip RNA 6000 kit (Agilent Technologies, USA) on a Bioanalyzer 2100 (Agilent Technology) Sample libraries were prepared using the QIAseq miRNA Library Kit (Qiagen, Germany) according to the manufacturer's guidelines and were then sequenced on an Illumina instrument (75-cycle single-end read, 75SE).