GSM7011479: Bend3_WGBS_KO3; Mus musculus; Bisulfite-Seq
Genomic DNA was extracted from the cells with QIAmp DNA Mini kit (Qiagen), according to the manufacturer's instructions. The library preparation for WGBS was performed with the tPBAT protocol described previously (Miura 2012, Miura 2019; NAR). One hundred nanograms of genomic DNA spiked with 1% (w/w) of unmethylated lambda DNA (Promega, Madison, WI) was served for the library preparation. The sequencing was performed by Macrogen Japan Inc. (Tokyo, Japan) using the HiSeq X Ten system.