GSM7049696: vH-PFC deep mouse a; Mus musculus; RNA-Seq
Neurons in vCA1 projecting to PFC were fluorescently labeled using retrobead injections into PFC. Slices of vCA1 were then manually dissected into superficial or deep layers. After trituration to create a single cell suspension, fluorescent neurons were isolated using FACS, and RNA harvested using SMART-Seq v4 Ultra Low Input RNA Kit (Clontech Laboratories, Inc.) 200pg of cDNA was then converted to sequencing library using the Nextera XT DNA protocol (Illumina, San Diego, US)