GSM6893975: DEN15d, replicate 2, scRNAseq; Mus musculus; RNA-Seq
Samples were processed as previously described in (Liu et al., 2015), and sorted by fluorescence activated cell sorting (FACS) to remove cell debris, doublets and dead cells. 100,000 live cells/sample were sorted and used for downstream processing with the 10X Genomics 3' v3 kit for single-cell gene expression. 12,000 cells were used for library preparation, a little over the 10,000 suggested by the 10X protocol, in order to reach a higher capture rate. Libraries were constructed per manufacturer's instructions and sequenced using Illumina's NextSeq platform.