GSM6025661: HepG2_IP; Homo sapiens; RIP-Seq
Total RNA was isolated and purified using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer's procedure. The RNA amount and purity of each sample was quantified using NanoDrop ND-1000 (NanoDrop, Wilmington, DE, USA). The RNA integrity was assessed by Bioanalyzer 2100 (Agilent, CA, USA) with RIN number >7.0. Poly(A) RNA was purified from total RNA using Dynabeads Oligo (dT) by two rounds of purification. The poly(A) RNA used in immunoprecipitation reactions with anti-YTHDF2 (abcam,ab220163) as IP. The IP/input RNA were reverse-transcribed to create the final cDNA library by using Stranded mRNA Library Prep Kit for Illumina following the manufacturer's instruction.