GSM7926308: 1-Embryons-WT; Danio rerio; RNA-Seq
Zebrafish larvae were euthanized with excess tricain. Heads were dissected and gently transferred into NbAct1 (Fisher # NC0319856) in 24 well-plates on ice. Tissues were washed 2 times in 1mL FACSmax (Genlantis #T200100) and incubated for 20min in FACSmax. Tissues were triturated (5 to 10 times) with 1mL tip to help physical dissociation, transferred into Eppendorf tubes and centrifuged at 500g for 5 min at 4°C. Pellet was washed with NbActiv1 medium, then passed through a 40 μm cell strainer (Corning), and centrifuge at 500g for 5 min at 4°C Library was performed according to the manufacter's instructions (single cell 3', 10x Genomics). 10X-based libraries were acquired with the Chromium Single Cell V3.0 reagent kit following the manufacturer's protocol (10X Genomics # 1000261, Cell Multiplexing Oligo Labeling).