Illumina NovaSeq 6000 paired end sequencing; Timecourse single-cell ATACseq of whole rabbit embryos sampled across gestational days 7, 8 and 9
Timecourse single-cell ATACseq of whole rabbit embryos sampled across gestational days 7, 8 and 9
Embryos dissected from the same time-points for transcriptional profiling were flash frozen by placing embryos into CorningÂ® Cryogenic Vials and immediately submerging the vials in liquid nitrogen. Embryos were stored in -80C and then shortly processed to extract nuclei following a 10X genomics demonstrated protocol for nuclei isolation for single cell ATAC on frozen tissues (CG000212). GD9 embryo was split into the extraembryonic portion for one well and the embryo portion was split across four wells of the 10X chip. 10X ATAC (v1.1 chemistry) according to manufacturers instructions 10X ATAC (v1.1 chemistry) according to manufacturers instructions