GSM6932521: TBIN #6, day2 [TBIN6_2d_s156]; Rattus norvegicus; miRNA-Seq
Whole blood was drawn from the lateral tail vein into Microtainer K2 EDTA-tubes. To obtain plasma, tubes were centrifuged at 1300xg for 10 min at 4 °C within 1 hour after blood sampling. RNA was extracted from 200 μl plasma using miRNeasy Mini Kit (#217004, QIAGEN). Small RNA library preparation was performed using the Illumina TruSeq Small RNA Sample Prep Kit. Briefly, small RNA was isolated from purified RNA by size selection after adapter ligation. The excised product was used for PCR amplification of the resulting product. The quality and yield after sample preparation were measured with a Fragment Analyzer. The size of the resulting products was consistent with the expected size of approximately 150 bp. Single-read sequencing with a read length of 51 nts was performed on a Illumina HiSeq 4000. To increase read depth each sample was sequenced on two flow-cells giving two replicates for each sample.