GSM7474597: A549-TIMM13_si1-rep2; Homo sapiens; ssRNA-seq
We constructed the dUTP-based strand-specific RNA-seq library by RNA HyperPrep Kit (KAPA). 500 ng purified total RNA of each sample were processed for poly (A)-enrichment and fragmentation. Then 1st strand cDNA and 2st strand cDNA were synthesized from fragmented RNA, followed by A-tailing and adapter ligation. Finally, adapter ligated library DNA was amplified by PCR Library concentration was determined by Qubit dsDNA HS Assay Kit (Invitrogen) according to manufacturers instructions