PacBio DNA-Seq of Bertholletia excelsa
RTL Genomics used the standard manufacturer's protocol to construct >30 kb size selectedSMRTbell libraries. The modifications to the standard library protocol were the following: the DNA was not sheared (it was 50kb  70kb after extraction) and the End Repair was extended to 30 minutes. The SMRTbell library was sequenced in 10 cells of the PacBio Sequel platform using chemistry 2.1.