RNA-seq of mustela nigripes ejaculate
Cell lysis, cDNA conversion, fragmentation, and library prep were done in single tubes using the QIAseq FX Single Cell RNA Library Kit (Qiagen, USA). Final library amplification was performed using Q5 Hot Start polymerase (New England Bioscience), utilizing indexing primers and universal Illumina adapters obtained from the Functional Genomics Lab (FGL) at the University of California, Berkeley. Final libraries, which were constructed for each individual sample, were submitted to the FGL for library quality check via Bioanalyzer (Agilent) and subsequent 150 bp paired-end sequencing on Illumina NovaSeq S4 flow cell, attempting 37 million reads per library.