VDJ Sequencing of Human B Cells: plasmablasts
PBMCs collected 1 week after one dose of the H1 stabilized stem ferritin vaccine from participant P30 were stained with B cell markers and fluorescently labeled influenza H1 hemagglutinin and H1 stabilized stem.  H1 stems-specific plasmablasts (upper population) were bulk sorted and cDNA was made using the 10x Genomics Chromium Next GEM Single Cell 5 Kit v1 per the manufacturers instructions. V(D)J genes were then amplified and libraries made for NGS sequencing using the v1 10X Genomics BCR amplification and library preparation kits according to the manufacturers instructions.