GSM7119382: Root, 5 dpg, PLT3; Arabidopsis thaliana; OTHER
Roots were cut on the agar, immediately frozen in liquid nitrogen, then ground with a metal ball in a TissueLyzer LT (Qiagen). CTAB DNA extraction was used on the remaining powder. DAP-seq libraries were prepared according to Bartlett et al., (2017) with Illumina TruSeq barcodes. 200 ng of gDNA was used as starting material for the PLT3 and EGFP samples. Libraries were amplified using 20 cycles. Post-amplification libraries were size selected at >200 bp in length using AMPure XP beads.