GSM6320949: PPARa GS; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a ultrasonicator (Covaris). DNA fragments were end-repaired, 3'A-tail added, and ligated to Illumina sequencing adapters. Libraries were amplified and post amplification libraries were size selected at 100-300bp in length using Agencourt AMPure XP beads from Beckman Coulter.