WGS of MmcA knockout mutant of Methanosarcina acetivorans
Cells from a 10 ml culture of DDN009 (mmcA KO) incubated in in HS + TMA at 37 oC were harvested at late-exponential phase (OD600 ~0.8) for genomic DNA extraction using the Qiagen blood and tissue kit (Qiagen, Hilden, Germany). The concentration of genomic DNA was measured using a Nanodrop One Microvolume UV-Vis Spectrophotmeter (Thermo Scientific, Waltham, MA, USA). Genomic DNA was shipped to the Microbial Genome Sequencing Center, Pittsburgh, PA, USA, where sequencing libraries preparation and sequencing was performed.