GSM7473752: CONTROL-replicate1; Homo sapiens; RNA-Seq
Single nuclei suspensions were prepared from flash-frozen human brain tissues using the Chromium Nuclei Isolation Kit with RNase Inhibitor (PN-1000494, 10x Genomics). Single nuclei suspensions were then submitted to a microscopic inspection of integrity and further processed according to manufacturer's (10x) protocols at a concentration of 4.000-8.000 nuclei/µl. Single nuclei mRNA libraries were prepared using the 10x Chromium Single Cell 3' v.3.1. Solution. Quality control of all cDNA samples was performed with a Bioanalyzer 2100 (Agilent Technologies) and libraries were quantified with the Qubit dsDNA HS kit (ThermoFisher).