RNA-Seq of Chaetoceros muellerii
Total RNA was extracted using TRIzolReagent (Invitrogen) and purified with the RNeasyMinEluteCleanup kit (Qiagen) according to the manufacturers instructions. For each treatment, two biological replicates were performed, each consisting of three technical replicates. For sequencing, eight cDNA libraries were generated. The constructed libraries were carried out for sequencing on Illumina NextSeq 500 platform, mode paired-end (2 x 150 bp). Sequencing was performed using Illumina TruSeq RNA sample v2 Guide preparation, according to the manufacturers instructions.