GSM6589680: HFSCs, CKO, Day26; Mus musculus; RNA-Seq
HFSCs were sorted, spun down and dissolved in TRIzol (Life Technology), and total RNA was harvested by means of the Direct-zol RNA MicroPrep Kit (Genesee Scientific). Total RNA quality was determined with a spectrophotometer (Thermo scientific). Avove 800 mg of total RNA of each sample was used for the construction of sequencing libraries. The corresponding library quality control protocol will be selected depending upon product requirements.