LncRNA-seq of Ciona intestinalis: adult ovary
Total RNA from Ciona tissues were extracted, purified, and treated with TURBO DNase (Thermo Fisher Scientific, USA). The quality-checked total RNA by the BioAnalyzer (Agilent Technologies, USA) was subjected to the library construction using Ribo-Zero Plus rRNA Depletion Kit (Human/Mouse/Rat; Cat No. 20037135) and NEBNext Ultra Directional RNA Library Prep Kit for Illumina(Cat No. 7420), according to the manufacturers instructions. 150-cycle of paired-end sequencing was performed using NovaSeq6000 (Illumina).