16S rRNA Seq: adult rectum
One portion of each sample was sequenced using 16Sv4 rRNA sequencing targeting the v4 region with primer 515F-806R, while another portion was sequenced using WMS. The 16S rDNA V4 region was amplified by PCR and sequenced on the MiSeq platform (Illumina) using the 2x250 bp paired-end protocol yielding pair-end reads that overlap almost completely. The primers used for amplification contain adapters for MiSeq sequencing and single-end barcodes allowing pooling and direct sequencing of PCR products.