GSM6809649: E4assem_scRNAseq; Homo sapiens; RNA-Seq
Cultured assembloides were dissociated with Adult Brain Dissociation Kit (Miltenyi) with manufacturer's protocol (37C_ABDK_02). Dissociated cells were stained with Fixable Viability Dye eFluorTM 700 (BD) for 30min, 4C for FACS sorting. FACS sorted cells were suspended with 0.04% BSA/PBS solution. Libraries were generated with the manufacter's instructions (single cell 3' v3.1 protocol, 10x Genomics).