GSM5603463: OCS scRNA-seq; Homo sapiens; RNA-Seq
After surgical resection, fresh samples were collected and washed with phosphate-buffered saline (PBS) for three times. Tissue was cut into approximately 1 mm3 pieces and enzymatically digested collagenase IV (Sigma), collagenase I (Sigma) and DNase I (Worthington) at 37°C for 30 min. After digestion, the sample was filtered out with a 35 µm cell strainer. Single cells were stained with AO/PI for viability assessment and live cells were preferentially sorted for single-cell sequencing. RNA libraries were generated by 10xGenomics Single Cell 3' V3.1 Library Gel Bead Kit and prepared for sequencing using standard Illumina protocols