SureSelect BS-Seq of Rattus norvegicus: 5_Irradiated_1.5month
Library preparation, probe-based target enrichment, bisulfite treatment, and library indexing PCR was performed according to SureSelectXT Methyl-Seq Library Preparation kit (Agilent Technologies Inc., USA) protocol (Version E0, April 2018). The libraries were then equimolarly pooled and sequenced together with a 20% PhiX control spike-in v3 (Illumina) on Illumina Hiseq 4000, generating approximately 1.2E+09 paired-end reads of 150 basepairs length.