nematode soil metagenome of Acacia saligna 10-30 late rainy season
Soil was collected beneath the canopy of the tested plants in two depths and different time periods. DNA was extracted from 0.5g soil of each sample using PureLink Genomic DNA Mini Kit (Invertogen, Thermo Fisher Scientific Inc., Waltham). PCR was conducted using CS1-NF1 and CS2-18sr2b.