GSM7474904: Age360d2; Sus scrofa; RNA-Seq
Approximately 5 μg of total RNA was used to eliminate ribosomal RNA according to the Ribo-ZeroTM rRNA removal Kit (Illumina, USA), and the ribo-minus RNA was used for construction of sequencing libraries. The 2×150 bp paired-end sequencing (PE150) was executed. Cutadapt was initially applied to obtain clean data, and Hisat2 software was used to map clean reads to the Sus scrofa reference genome (V11.1, Ensembl V96), and the mapped reads were assembled using StringTie.