GSM7089527: GSC040815_RCN3 knockdown_2; Homo sapiens; RNA-Seq
RNA was isolated using RNeasy mini kit and subjected to RNA-seq on illumina NextSeq 500 system using Illumina TruSeq stranded mRNA-seq library preparation kit Total RNA samples about 500ng were used for sequencing library preparation according to NEB stranded mRNA-seq Sample Preparation Guide (NEB Cat. #: 7765). Each library was bar-coded and then pooled for cluster generation and sequencing run with 75bp single-end (SE) sequencing protocol.