GSM7119442: CD8+ T cells STAT3- CM  replicate #1; Mus musculus; RNA-Seq
RNA was harvested using PicoPure RNA isolation Kit (ThermoFisher). Total RNA samples (500ng), with RNA Quality score of 9.4 on average (range 9.0-9.8 on a PerkinElmer LabChip analyzer), were converted into sequencing libraries with the "NEBNext Ultra II Directional RNA Library Prep Kit for Illumina" (NEB #E7760). Briefly, polyA+ fraction is purified and randomly fragmented, converted to double stranded cDNA and processed through subsequent enzymatic treatments of end-repair, dA-tailing, and ligation to adapters. Adapter-ligated library is completed by PCR with Illumina PE primers. The resulting purified cDNA libraries were applied to an Illumina flow cell for cluster generation and sequenced on an Illumina instrument by following manufacturer's protocols.