GSM8047854: Injury NMDA 36hr, snRNA-seq; Danio rerio; RNA-Seq
Zebrafish retinas (4-6 retinas from 4-6 fish) were collected at different timepoints after NMDA and LD treatments, and flash-frozen in dry ice for ~15min before being transferred to a -80 ? freezer for storage. Fixed cells were recovered and subjected to 10x Genomics Single Cell protocol as described previously. Libraries were prepared and sequenced on Illumina NovaSeq at ~500 million reads per library.