GSM6934360: Sperm, B2026W, winter; Bos taurus; Bisulfite-Seq
Sperm were collected after thawing the frozen semen straw at 37 ºC temperature The genomic DNA spiked with lambda DNA were fragmented to 200-400bp with a Covaris s220 sonicator followed by terminal repair and adenylation. Lambda DNA was used as unmethylated control for bisulphite conversion rate calculation. Cytosine methylated barcodes were then ligated to the sonicated DNA and treated twice with an EZ DNA Methylation-Gold™ Kit (Zymo Research) for bisulfite conversion.