GSM7838587: Retinal ECs, BMP9-10 Abs-treated, replicate 1; Mus musculus; RNA-Seq
Eyes from P7 pups of control (group 1), BMP9/10 Abs-treated (group 2), and BMP9/10 Abs + CDK4/6i (group 3) treated animals were dissected out and primary retinal ECs were FACS-isolated for bulk RNA sequencing. Six to nine retinas/group were pooled for 1 experiment and each experiment was repeated 3 times (N=3). mRNA samples from retinal ECs from groups 1, 2 and 3 were isolated using RNeasy Plus Micro Kit (QIAGEN, cat# 74034). RNA libraries were prepared as per the (HiSeq4000) manufacturer's protocols. Next-generation whole transcriptome Illumina sequencing was performed at Yale Center for Genome Analysis.