GSM7089531: Omentum naïve, replicate 3; Mus musculus; RNA-Seq
Tumor-bearing omenta were excised 24 hours after treatment. Tumor-bearing omenta were storaged in RNAlater at -80C until further processing.mRNA was isolated using Rneasy Mini Kit (Qiagen) following manufacture's instructions. Library preparation and sequencing was developed by Unidad de Genómica (NGS) CIMA- Universidad de Navarra. Library preparation was performed using the Illumina Stranded mRNA Prep Ligation kit following the manufacturer's protocol. All sequencing libraries were constructed from 100 ng of total RNA.