WES of human: PBMC male
fragment libraries using either 200 ng or 3 ug of genomic DNA were constructed from each sample, following the Agilent standard library preparation protocol for TruSeq (Illumina), SureSelectXT Reagent kit for 96 samples (Cat# G9611B). Exome capture was done with the SureSelectXT Human All Exon V5+UTRs library (96 samples) (Agilent, Cat# 5190-6214), and sequencing was done using the HiSeq 2000 or 2500 (Illumina) at the Columbia Genome Center. On average, approximately 120-150 million independent paired-end reads were generated per sample to provide an average coverage of at least 75-fold (normal) and 150-fold (IOP), with ->98% of target regions being covered at least 15-fold