GSM7925857: LFDxCast_WT; Mus musculus; RNA-Seq
Spleens were isolated on ice and homogenized between two glass slides. The single-cell suspension was filtered through 70 µm and 30 µm strainers and incubated with Fc-block for 15 min. Then cells were stained with Zombie Green (Viability, BioLegend) and a TER-119-PE antibody (Erythrocytes, ThermoFisher). After staining, cells were incubated with Cell Multiplexing Oligos (CMO, 10X) to add a specific barcode to each sample, allowing the pooling of all four samples in one 10X reaction. The Zombie Green-negative and TER-119-negative population were FACS-sorted and counted. Single-cell library was generated using the Chromium Next GEM Single Cell 3' Reagent Kits v3.1 (Dual Index) with Feature Barcode technology for Cell Multiplexing (10X).