Construct PTS40; transformation event 6; chloroplast-expressed convergent promotor producing doubled-stranded RNA targeting PDS tobacco nuclear gene; whole-cell RNA; sRNA raw reads; biological replicate 3
Whole-cell total RNA was isolated from whole seedling at early first leave stage excluding roots; sRNA libraries were constructed using the RealSeq-AC from Somagenics using 150 ng of total RNA and amplified with 16 PCR cycles. Sequencing was done with the Illumina NextSeq 550.