RNA-seq of Solanum tuberosum: recovery tolerant root rep 2
total RNA was extracted from 1-2g of material using TriReagent (Sigma) followed by treatment with DNAase using the DNA-freeTM (Ambion) kit. Sample purity and concentration was determined by the OD260/OD280 and OD260/OD230 ratios using NanoDropTM and sample integrity was verified with agarose gel electrophoresis. mRNA library construction and sequencing of all 48 samples was carried out at Michigan State University