GSM6729659: WT_E14_mESCs; Mus musculus; ATAC-seq
In brief, 50,000 mESCs were collected in cold PBS and lysed with a 2x lysis buffer (Tris-HCl pH7.5 1M, NaCl 5M, MgCl2 1M, 10% IGEPAL). Next, cells were pelleted and incubated with 2xTD buffer and 2 uL transposon mix. Next, PCR amplification was carried twice by KAPA HiFi HotStart ReadyMix using P5 and P7 indexed primers (see oligonucleotide list). Fragments between 200 and 700 bp were purified using AMPure XP beads. Quality control was performed by Bioanalyzer High Sensitivity DNA analysis (Agilent).