ssRNA-seq of Mus musculus: CD44lowGFPpos T cells
Three immune mice receiving allogeneic heart transplants were sacrificed after transplantation. Hearts were digested and pooled together for cell staining. The FACSymphony S6 (BD Biosciences) was used to sort for CD44lowGFP+ cell population. The sample was processed for single-cell sequencing using the Chromium Next GEM 5v2 Single Cell V(D)J Reagent Kit protocol. Libraries of enriched TCR sequences were created using the Single Index Kit Set A (10X Genomics) and sequenced on the NovaSeq 6000 (Illumina).