GSM6809592: WRENF_1; Mus musculus; RNA-Seq
RNA isolation of organoids and tissues were performed with RNeasy Mini Kit (QIAGEN) following the manufacturer's instructions. Reverse transcription was performed using Maxime RT PreMix (#25081; iNtRON, Korea). A library was constructed using TruSeq RNA Library Prep Kit (Illumina) from total RNA and sequenced on an Illumina HiSeq 2000 sequencer.