GSM6620235: NTERT-WT-ctrl2; Homo sapiens; RNA-Seq
Total RNA content was collected and purified using the Rneasy kit (Qiagen) following the manufacturer's directions Library preparation, polyA selection, and sequencing were performed as follows. Briefly, RNA samples were quantified using a Qubit 2.0 fluorometer (ThermoFisher Scientific) and RNA integrity assessed with a 4200 TapeStation (agilent Technologies). Samples were then normalized using the ERCC RNA Spike-In Mix kit (ThermoFisher Scientific) following the manufacturer's recommendations. The RNA sequencing library was prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturer's recommendations.