GSM7089926: U2OS wildtype cells, no UV, biol rep2; Homo sapiens; ATAC-seq
DNA to be tagmented was processed according to the Omni-ATAC-seq protocol by Corces et al. 2017 (doi: 10.1038/nmeth.4396). 50k cells were pelleted, incubated in ATAC-seq lysis buffer, washed, and re-pelleted; nuclei were exposed to the transposition reaction using Tn5 pre-loaded with Illumina adapters. Purified transposition reactions were PCR-amplified with custom ATAC-seq primers using the minimum number of PCR cycles determined from qPCR. The resulting libraries were purified and size selected with AMPure beads, analyzed on a TapeSttation to verify desired fragment size range, pooled at equimolar concentration, and submitted to the Functional Genomics Center Zurich to be sequenced as 50 bp paired-end reads on a NovaSeq 6000.