GSM6320817: ATACseq P. flava early blastula replicate 2; Ptychodera flava; ATAC-seq
Animals at different stages were collected to reach 75,000 cells per sample, and treated with 0.1% N-acetylcysteine for 10 sec to remove the jelly coat, followed by incubating with 8.5 mM of DTT in seawater containing 5.7 mM of NaOH. The cells were then lysed for the transposition reaction and PCR amplification. Transposition with Tn5 enzyme and PCR amplification.