GSM7976858: fip373A-m6A_rep1; Arabidopsis thaliana; OTHER
Seedlings were homogenized in liquid nitrogen, and RNA was harvested using Trizol reagent. PolyA mRNA was isolated by oligodT beads and further fragmented. The m6A containing RNA was isolated with antibody. RNA libraries were prepared for sequencing using standard Illumina protocols. m6A-seq libraries were prepared according to Shen, L. et al., Developmental Cell, 2016. Briefly, RNA was converted to final cDNA libraries in accordance with a dUTP dependent strand-specific library preparation. Libraries were sequenced on the Illumina Nova 6000 platform as single-end 150 bp reads at the OE Biotech (Shanghai, China) following the vendor's recommended protocol.