GSM6025407: HiC_AML12_shCtrl_rep1; Mus musculus; Hi-C
Cells (2.5 billions/sample) were crosslinked by 1% formaldehyde at 25℃ for 10 minutes. Then the cell chromatin was digested with 100U MboI at 37℃ overnight. Subsequently, filled in the restriction fragment overhangs with biotin at 37℃ for 1.5 hours and performed proximity ligation at room temperature for 4 hours. After protein degradation and crosslink reversal, DNA was purified by ethanol precipitation and were sheared to a size of 300-500bp. After size selection, the biotinylated DNA was pulled-down by streptavidin beads and then prepared for Illumine sequencing.