WXS of Human specie
GenomicDNAisfirstfragmented,coupledwiththesplicesequence,andcyclizedtoformsingle-strandedcircularDNA.Thentherollingcircleamplification(RCA)canamplifythesingle-strandedcircularDNAby2-3ordersofmagnitude.TheresultingamplificationproductiscalledDNAnanoball(DNB),andthefinalnanosphereisfixedonthearrayedsiliconchipthroughDNBloadingtechnology.