GSM7474440: Biol Rep WT_2; Mus musculus; miRNA-Seq
Total RNA was isolated using miRVana miRNA isolation kit (Ambion, cat no AM1560) according to manufacturer's instructions. The multiplexed miRNA libraries were prepared as described in detail in Eminaga et al, 2013 (Curr Protoc Mol Biol. 2013 Jul;Chapter 4:Unit 4.17) and Vigneault et al 2012 (Curr Protoc Hum Genet. 2012 Apr;Chapter 11:11.12.1-11.12.10).