NGS of PCR products of mus musculus: adult male bone marrow
Purification of PCR products were performed using Zymoclean Gel Recovery Kit (D4008, Zymo Research) and DNA samples were quantitated by Qubit 2.0 Fluorometer (Thermo Fisher Scientific) before NGS library preparation using NEB DNA Library Prep Kit for Illumina (E7645). The raw data were obtained by Illumina Hiseq2500 system (PE250), and the data were processed and analyzed by using CRISPResso2. For each sample around 100,000 effective reads were obtained from NGS of the PCR products.