GSM7748858: Y16: siRAPTOR_replicate #1; Homo sapiens; RNA-Seq
RNA of human dermal endothelial cells treated with control or RAPTOR siRNA was extracted using an RNeasy Mini Kit (Qiagen) per manufacturer's instruction. RNA samples were quantified using Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA, USA), and RNA integrity was measured using the RNA Screen Tape on Agilent 2200 TapeStation (Agilent Technologies, Palo Alto, CA, USA). The RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer's instructions (New England Biolabs, Ipswich, MA, USA). The sequencing libraries were multiplexed and clustered on 1 lane of a flowcell. After clustering, the flowcell was loaded on the Illumina HiSeq 4000 instrument according to manufacturer's instructions.