GSM7179264: RNAseq_GSC2_t_0_S72; Homo sapiens; RNA-Seq
total RNA was lysed and purified by Trizol extraction. RiboRNA was performed extracted using a buffer consisting of 20 mM Tris pH 7.5, 150 mM NaCl, 5 mM MgCl2, 100 µg ml-1 cycloheximide, 1% Triton X-100, 10 U ml-1 Turbo DNase I and nuclease free H2O mRNA-seq libraries were generated from high quality RNA (average RNA Integrity Number (RIN) of 9.5)