GSM6319583: tg.7d_2; Mus musculus; RNA-Seq
Small intestinal tissues were disrupted using Precellys 24 tissue homogenizer. Isolation of RNA from small intestinal tissues was performed using a NucleoSpin RNA isolation kit from Macherey Nagel according to manufacturer's instructions. RNA quality was evaluated based on RNA integrity number (RIN) and OD260/280 and OD260/230 ratios. RIN value was determined using TapeStation4200 and RNA Screen Tapes from Agilent Technologies. Lexogen 3' QuantSeq Kit (FWD protocol).