GSM6590725: EPISC replicate2 [HiChIP]; Mus musculus; OTHER
5ng of immunoprecipitated DNA material was used to make libraries using the Swift Biosciences Accel-NGS 2S Plus DNA Library Kit (Cat #21024) according to manufacturer's instructions and performing between 8-14 cycles of amplification for all samples. ESC cells were processed for each HiChIP replicate using the Abcam H3K27ac antibody (ab4729) and following the HiChIP protocol as previously described (Di Giammartino et al., 2019). TSC, XEN and EpiSC cells were used for each HiChIP replicate using the Arima-HiC+ kit (Arima, A101020) and the H3K27ac antibody (active motif H3K27ac 91193) according to manufacturer's instructions with few modifications.