GSM7120307: Not infected, 5°C, replicate 3; Myotis myotis; RNA-Seq
After a total of 54h post-infection (hpi), the supernatants were collected and stored at −80◦C. The cells were lysed with 1 mL of TRIzol (ThermoFisher) and RNA was extracted using Direct-zol RNA Miniprep Kit (Zymo Research) according to the manufacturer's instructions (including a DNAse I treatment), and eluted into 50 µL RNAse free water. Strand-specific single-end cDNA libraries were prepared using TruSeq Stranded mRNA sample prep kit, Illumina according to the manufacturers' instructions and sequenced using Illumina NextSeq 500 sequencer.