GSM7799888: GSC_Stag3KO2_RNA_2; Mus musculus; RNA-Seq
Total RNA was extracted from cells using a RNeasy Micro kit (QIAGEN, 74004) according to the manufacturer's instructions. 1 ng of extracted RNA was used for the reverse transcription and the cDNA amplification as previously described  (Nakamura et al, 2015). Sequencing libraries were generated from amplified cDNA as described previously   (Nakamura et al, 2015).