GSM7383627: Con-d21, male, rep2 (RNA-seq); Mus musculus; RNA-Seq
Ground livers from d0 and d21 male (n = 5) mice were used for total RNA isolation by using a miRNeasy Mini Kit (Qiagen) following the manufacturer's instructions, including an on-column DNase I treatment. RNA was quantified spectrophotometrically. The integrity of RNA was assessed with an Agilent 2100 bioanalyzer in conjunction with the RNA 6000 Nano Kit (Agilent), and a RNA integrity number greater than or equal to nine was used. RNA libraries were prepared by using 200 ng of total RNA according to the manufacturer's instructions for the Illumina TruSeq RNA v2 kit