GSM8047581: BAP treated prime cells (GEX); Homo sapiens; RNA-Seq
Cells were dissociated with TrypLE Select (4min at 37°C; ThermoFisher Scientific, 12563011) and deactivated by adding 3 times more volume of NutriStem hPSC XF. Cells were collected in 0.04% BSA (Sigma-Aldrich, A7979) in PBS-/- and used following the Cell Multiplexing Oligo (CMO) Labeling for Single Cell RNA Sequencing Protocols with Feature Barcode technology protocol (10x Genomics, CG000391). Following cell count and verification of live cells using the NC-200 Nucleocounter (Chemometec) of individual samples and the final pool  (4 CMO samples combined), we then used the Chromium Next GEM Single Cell 3′ Reagent Kits v3.1 (Dual Index) with Feature Barcode technology for Cell Multiplexing (10x Genomics, CG000388) according to the recommended user guide. A 2000 target cell recovery was aimed for each individual sample. CMO libraries and transcriptome libraries were sequenced using Illumia NextSeq 2000.