GSM7179652: mTSC_siNC MeRIP-seq rep 1; Mus musculus; RIP-Seq
Total RNA was extracted using Trizol reagent (Invitrogen)  following the manufacturer's procedure. The total RNA quality and quantity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit. RNA libraries were prepared for sequencing using standard Illumina protocols. RNA fragmentation Reagents (Ambion) was used to randomly fragment RNA. m6A-specific antibody (Synaptic Systems) was applied for m6A pull down. Then, final library preparation was constructed.Finally, we performed the paired-end  sequencing on an Illumina NovaSeq 6000 platform following the vendor's recommended protocol. MeRIP-seq