GSM7384113: Tomato root, galls, Day21, RNA_rep 3; Solanum lycopersicum; RNA-Seq
Root tissues of 14-day-old (non-infected) and 4 dpi (M. incognita infected) plants were collected and frozen immediately in liquid nitrogen. Total RNA was extracted using Direct-zol TM RNA Miniprep Plus (R2070S). Same protocol was repeated for 11 dpi infected and non-infected replicates. For the construction of a stranded and ribosomal depleted library, mature mRNAs were processed and captured using oligo(dT) beads following the addition of a 3' poly(A) tail.