GSM6934170: JHH-6 cells transiently transfected with 100 pmol siRNA APE1, replicate 3; Homo sapiens; RNA-Seq
RNA extraction was carried out using miRNeasy kit (Qiagen, USA) following the manufacturer's protocols. Library preparation was performed using SMARTer smRNA-Seq kit (Clontech/Takara, USA) protocol with minor changes to increase the chance of identifying also pri-miR in addition to miRNAs. RNA-Seq on a Illumina HiSeq 2500  instrument, in rapid run mode, using a 100-bp, dual-indexed, single-end sequencing configuration