Efficient discovery of potently neutralizing SARS-CoV-2 antibodies using LIBRA-seq with ligand blocking
LIBRA-seq with ligand blocking on PBMCs from a recovered SARS-CoV-2 human donor sample. Human PBMCs were stained with fluorescently labelled antibodies and oligo-tagged antigens (including SARS-CoV Sand SARS-CoV-2 S) and ACE2, sorted via FACs, and processed for single cell sequencing with the 10X Genomics Chromium Controller (5'VDJ kit with antigen barcode processing). The VDJ and antigen libraries were sequenced on the Novaseq (Illumina).