GSM6319727: HGC-27, GC0643, replicate 3; Homo sapiens; RNA-Seq
The total RNA from HGC-27GC0643 and HGC-27Vector was harvested using Total RNA Extraction Reagent(Vazyme, China) . 2μg of total RNA was used for the construction of sequencing libraries. Qubit RNA Assay Kit in Qubit 2.0 Flurometer (Agilent Technologies,USA) was used for preliminary RNA quantification. And RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system(NEB,USA) was used for measuring RIN value.NEBNext® Ultra RNA Library Prep Kit for Illumina(NEB,USA) was used for library construction.