GSM6729962: human_tonsil_organoid_06yrs_F_COVID_vaccine_TLR7NP_day04and09; Homo sapiens; RNA-Seq
Cell capture and library preparation were completed using the BD Rhapsody Targeted mRNA and AbSeq Reagent Kit (Cat. # 633774). Briefly, cells were captured with beads in  microwell plate, followed by cell lysis, bead retrieval, cDNA synthesis, template switching and Klenow extension, and library preparation following the BD Rhapsody protocol. Libraries were prepared for sample tags, targeted mRNA using the customized Immune Response panel, and AbSeq.