GSM6893767: Isotype mAb (CW015); Mus musculus; RNA-Seq
Miltenyi neural dissociation kit (P) used to dissociate the tissue into a single cell suspension. Following myelin removal, samples were stained with CD31 FITC, CD11b PE-Cy7, DRAQ5 and propidium iodide, and live nucleated CD31+ or CD11b+ cells were collected by FACS for single-cell RNA sequencing 10x Genomics Chromium 3' v3 chemistry