GSM6729523: GM, siSTAT5B (Rep 2); Homo sapiens; RNA-Seq
total RNA was isolated from macrophages using the Micro RNeasy kit (Qiagen) according to the <100,000 cells protocol. RNA quality and integrity were assessed using a TapeStation System (Agilent). The NEBNext® Poly (A) mRNA Magnetic Isolation Module and Ultra II Directional RNA Library Prep Kit for Illumina (NEB) was used for the construction of sequencing libraries, which were then sequenced with the Illumina NextSeq 2000 System and NextSeq P3 sequencing reagent kit in a single read-mode with a read-length of 72 bp and dual unique index sequences.