GSM6932365: Individual B, clone 4, passage 39, basal medium; Homo sapiens; RNA-Seq
Total RNA was extracted from CStCs using Direct-zol RNA Kit (Zymo Research), according to manufacturer's instructions. The concentration and purity of the isolated total RNA was assessed by Nanodrop and Qubit, while the integrity was evaluated using the Experion RNA StdSens Analysis Kit (Bio- Rad). High quality RNA, with A260/A280 > 1.8, A260/A230 ranging from 1.8 up to 2.02 and RQI > 9.5, were used for subsequent library preparation. 500 ng of RNA for each sample were used for the preparations of the libraries, using the QuantSeq 3' mRNA-Seq Library Prep FWD (Lexogen). Samples were sequenced using the platform HiSeq2500, following the protocol SR1000.