GSM7119060: MB361-dCas9-KRAB+chr13:32639465-32639967_Rep3__; Homo sapiens; RNA-Seq
Cells were collected, washed once with PBS and extracted the RNA with Zymo Quick-RNA miniprep kit. 100 ng of RNA was used to build an RNA-seq library with the chemistry of 10x Chromium Next GEM Single-cell 3' Reagent Kit. 10 uL of beads were used in each reaction. The remaining steps of cDNA library preparation were identical to the 10x protocol with 0.25 fold of the reaction volume.