mRNA-seq of Homo sapiens
Total RNA of each sample was reverse transcribed intofirst-strand of cDNA, followed by double-stranded cDNA synthesis and amplificationof cDNA libraries by PCR using Illumina TruSeq RNA Sample Preparation Kit(Illumina, San Diego, CA, USA). After quality inspection, cDNA libraries wassequenced on Illumina HiSeq 2500 platform with pair-end 150 strategy.