High throughput sequencing of human airway epithelia AAV6.2 2
PCR products amplified from genomic DNA were barcoded by 12 cycles of PCR with unique combinations of barcoding primers for each sample, and these barcoded PCR products were pooled. Amplicons were purified after extraction from a 1% agarose gel following electrophoresis to remove primers. Extracted DNA amplicons were quantified using a QuBit High Sensitivity assay and normalized to 4nM. Sequencing was conducted using an Illumina MiSeq v2 300 cycle kit as per manufacturer's instructions.