whole genome shotgun sequencing using Illumina Truseq from leaf tissue of Solanum tuberosum clone DM1S1
Genomic DNA for whole-genome shotgun sequencing (WGS) was isolated from young leaves of tissue culture grown DM1S1 and 1S1 clones using the DNeasy Plant Mini Kit (Qiagen, Hilden, Germany). Illumina TruSeq Nano DNA WGS libraries were prepared and multiplexed using IDT Illumina Unique Dual Index adapters, then sequenced on an Illumina HiSeq 4000 in paired-end mode by the Michigan State University Genomics Core, generating 150 nt reads