GSM6932396: liver, rapid reoxygen_12h,1; Trachinotus blochii; miRNA-Seq
The total RNA was extracted from the liver by the Trizol Reagent (Invitrogen). RNA degradation and contamination were monitored on 1% agarose gel. The RNA purity was assessed using a NanoPhotometer® spectrophotometer (IMPLEN, München, Germany). The RNA concentration was measured using the Qubit® RNA Assay Kit in the Qubit® 2.0 Fluorometer (Life Technologies, CA, USA). The RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). All RNA OD ratios of 260/280 and 260/230 were greater than 1.8, and the RNA integrity number (RIN) was greater than 8.0.We used Qubit 2.0 for preliminary quantification, diluted the library to 1 ng/uL, and used the highly sensitive Agilent 2100 to detect the insert size of the library. The different libraries were pooled according to the requirements of the effective concentration and target data volume, and the Illumina SE 50 was used for sequencing.