GSM8083499: tomato seedlings, K23 & salt, rep3; Solanum lycopersicum; RNA-Seq
The RNA from the plant material  was extracted  with 1 ml of TrizolTM  and material was incubated on a shaker at room temperature for 15 min. After the addition of 250ml. l chloroform and a second incubation phase, the sample was centrifuged (30 min, 4°C). The supernatant was mixed with isopropanol and incubated on ice, followed by centrifugation. The pellet was washed twice with 80% ethanol, dried and resuspended in RNAse-free water.  The quality and concentration of the extracted RNA were tested spectrophotometrically. Then the RNA integrity was evaluated using a bioanalyzer (Bioanalyzer 2100, Agilent) Library construction was done by Novogene Genomics, UK