GSM5729867: single organoids; Homo sapiens; RNA-Seq
Single, fused, and connected organoid at 7 weeks of culture on PDMS-MEA chip were first extracted from PDMS device in warmed maintenance medium and centrifuged at 100 xg for 30 seconds. To get single-cell suspensions, the organoids were dissociated by AccuMax for 10-30 min at 37°C and then centrifuged 200 xg for 5 min. Then, the pelleted cells were re-suspended in DMEM containing 10% FBS to be subjected to 10x Genomics Chromium single-cell RNA-seq library preparation, according to the manufacturer's protocol. Finally, the library was sequencing with 150-bp paired-end reads on DNBSEQ.