RNA-Seq of Leucaena leucocephala subsp. glabrata
Whole organisms were harvested as young plants and were immediately placed in liquid nitrogen. Total RNA extraction was performed on the harvested organisms using Qiagen's Plant RNeasy protocol, and the RNA purity was confirmed with NanoDrop Spectrophotometry. The library was prepared using Illumina's TruSeq-stranded-mRNA sample preparation protocol. mRNA was purified with two rounds of Oligo-dT magnetic bead capture and then fragmented in a divalent cation buffer. RNA purity was again confirmed using Agilent Technologies 2100 Bioanalyzer High Sensitivity DNA Chip. Paired-ended sequencing was performed on the pure RNA using Illuminas NovaSeq 6000 sequencing system.