GSM7118616: PC9 cells, Nil Unsorted  biol rep 1; Homo sapiens; OTHER
gDNA was recovered from fixed cells utilizing Arcturus PicoPure DNA Extraction Kit, according to manufacturer instructions. DNA-seq (sgRNA) libraries were PCR-amplified from gDNA using NEBNext Ultra II Q5 Master Mix (New England BioLabs) according to manufacturer instructions, using the following primers: FWD 5′- AATGATACGGCGACCACCGAGATCTACACAATTTCTTGGGTAGTTTGCAGTT, Rev 5′- CAAGCAGAAGACGGCATACGAGAT(6 bp index sequence)GACTCGGTGCCACTTTTTCAA. Amplified libraries were purified using SPRIselect beads (Beckman Coulter) using right-sided selection of 0.75x then to 1.2x the original volume. Each sample was quantified using the Quant-iT dsDNA Broad Range Assay Kit (Thermo). Samples were pooled and sequenced on a NextSeq 500 (Illumina) with 20bp single-end sequencing using the following custom read and index primers: Custom Read 5′- GATTTCTTGGCTTTATATATCTTGTGGAAAGGACGAAACACCG, Custom Index 5′- GCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTC. DNA-seq