Nanopore raw read
Leaves from a 30-day mature plant were harvested and frozen immediately in liquid nitrogen. Genomic DNA was extracted using a QIAGEN Genomic Kit and used as material to construct sequencing libraries following the standard protocols of Oxford Nanopore Technologies. Briefly, DNA was size-selected using a BluePippin instrument (Sage Science, USA) before end-repair and adaptor ligation on the resulting blunt ends. Finally, libraries were sequenced on a PromethION platform.