GSM7838814: H3K4me3, Intestine, FA; Oncorhynchus mykiss; ChIP-Seq
20–30 mg of tissue powder was cross-linked with 1% formaldehyde for 8 minutes, and quenched for 10 minutes with 100 μl of glycine. Centrifugation at 2000 g for 5 min yielded the nuclei, which were then resuspended in 600 μl of iS1 buffer and incubated for 30 min on ice. Depending on the tissues, the Bioruptor Pico sheared chromatin between 10 and 15 cycles. Libraries were constructed using the NEBNext Ultra DNA library prep kit (New England Biolabs #E7645L, Ipswich, MA)