GSM6932852: AP2-XII-1-KD-HA_AP2-XI-2-KD-Myc_IAA24h_replicate1; Toxoplasma gondii; ATAC-seq
Nuclei preparation, permeabilization, Tn5 transposition and library preparation was prepared following precisely the Diagenode ATAC-SEQ kit protocol (C01080002) Following the Tn5 reaction, libraries were amplified using the Diagenode 24 UDI kit 1 (ref 01011034) following standard protocol procedures. Libraries were multiplexed and sequenced on a single Novaseq6000 lane by Fasteris (Genesupport SA) using 2*50 cycles, generating on average 27 million reads.