GSM5750568: alcohol-FMT 18; Mus musculus; RNA-Seq
Total RNA was extracted from the testicular tissues of animals in the alcohol-FMT and control-FMT groups, with three replicates in each group. RNA integrity was assessed using an RNA Nano 6000 Assay Kit and a Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). The cDNA libraries were prepared using an Illumina TruSeq RNA sample prep kit. The average size of the library cDNAs was 150 bp (excluding adapters). The integrity and quality of cDNA libraries were assessed using an Agilent 2100 Bioanalyzer and an ABI StepOne Plus real-time PCR system. RNA libraries were prepared for sequencing using standard Illumina protocols