PacBio Iso-Seq of U937 (CCS)
First-strand cDNA was synthesized using the Clontech SMARTer PCR cDNA Synthesis Kit and then further amplified by PCR. The final library was sequenced on the PacBio Sequel II System with SMRT Cell 8 M and Sequel II Binding Kit2.0/Sequencing Kit2.0 (Pacific Biosciences).