GSM5750606: Epidermal_piRNAs_Rep1; Schmidtea mediterranea; ncRNA-Seq
RNA was extracted by guanidinium thiocyanate-phenol-chloroform extraction (TriFast (peqlab)). RNA was then purified and size-selected (<200 nt) using the RNA Clean & Concentrator Kit (Zymo Research Europe) according to the manufacturer's manual. Small RNAs were oxidized using  NaIO4. Library preparation with UMI-containing adapters was performed as described (Fu et al., 2018).