Construct PTS40; transformation event 6; chloroplast-expressed convergent promotor producing doubled-stranded RNA targeting PDS tobacco nuclear gene; chloroplast-enriched RNA; sRNA raw reads; biological replicate 2
Chloroplast were isolated from 250 mg of tobacco seedling leaves using the MinuteTM Chloroplast prior to isolate RNA; sRNA libraries were constructed using the RealSeq-AC from Somagenics using 150 ng of total RNA and amplified with 16 PCR cycles. Sequencing was done with the Illumina NextSeq 550.