GSM7089940: U2OS wildtype cells, no UV, biol rep2, input [paired-end]; Homo sapiens; ChIP-Seq
Non-UV or 3h-post UV cells were crosslinked with 1% formaldehyde for 10 minutes. Quenching and lysis was followed by sonication (BioRuptor Plus) for 20 cycles (30 sec on/off). To remove UV-induced DNA lesions with the potential to inhibit sequencing, samples were incubated with PreCR Repair mix (New England BioLabs, M0309) for 15 min at 37°C, then re-purified, quantified by Qubit (Invitrogen, Q33238) and sent to the Functional Genomics Center Zurich for final library preparation with the NEB Next Ultra Kit (New England BioLabs, E7645). Libraries were sequenced as 100 bp single-end reads on a NovaSeq 6000 (Illumina). Topped up reads for the H3K36me2 experiment were sequenced as 150 bp paired-end reads (NovaSeq 6000).