Illumina NovaSeq 6000 paired end sequencing; Transcription profiling of human dorsal forebrain organoids treated with Hyper-IL-6
Transcription profiling of human dorsal forebrain organoids treated with Hyper-IL-6
Organoids were homogenized in the RLT buffer from RNeasy MiniKit (Qiagen, Cat. no.74104) on ice, and stoted at -80C until RNA extraction. mRNA was isolated from organoids using the RNeasy MiniKit (Qiagen, Cat. no.74104) following manufacturerâ€™s instructions non-directional poly-A enrichment