GSM6685430: Sample_GTM3_1; Homo sapiens; RNA-Seq
Total RNA was extracted by using the manufacturer's protocol of mirVana miRNA Isolation Kit (Ambion). Agilent 2100 Bioanalyzer (Agilent Technologies) was used to evaluate the RNA integrity. Samples with RNA integrity number ≥ 7 were reserved further analysis. TruSeq Stranded mRNA LT Sample Prep Kit (Illumina) was used to construct libraries according to the manufacturer's instructions. Then, these libraries were sequenced on the Illumina sequencing platform (Illumina HiSeq X Ten) and 150 bp paired‐end reads were generated.