GSM7712430: HNRNPD_WT_nascent_SW839_rep2; Homo sapiens; RIP-Seq
RNA was extracted with Trizol. The rRNA was removed by Illumina Ribo-Zero Gold kit and surplus RNA was purified for end repair and 50-adaptor ligation. Then, reverse transcription was performed with random primers containing 3' adaptor sequences. Finally, the cDNAs were purified and amplified with PCR reaction. The products with 300-500 length were purified and quantified. These libraries were utilized to 150 nt paired-end sequencing with an Illumina Nova seq 6000 system (Novogene, China).