RNA-Seq of Ruditapes philippinarum:digestive gland
Total RNA from digestive gland was isolated using TRIzol reagent (Invitrogen) according to the manufacturer' s instructions. RNA samples were used if no smear was obvious on the monitored on 1% agarose. RNA concentration was analyzed using Nanodrop (Thermo Fisher Scientific), and the integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies). RNA samples conformed to the required purity criteria A260/A280 > 2.0 and quality levels RIN >7 for cDNA library preparation. The library preparations were sequenced on an Illumina Hiseq X Ten platform and 164 bp paired-end reads were generated and all raw sequence read data were stored in the Fast Q format.