GSM8084128: Smart-seq3 384 plate5; Danio rerio; RNA-Seq
Two zebrafish lines Gt(foxd3-mCherry)ct110R and Gt(foxd3-Citrine)ct110 were crossed in late afternoons. Embryos were collected the next morning and incubated until desired stages. Embryos were dissociated with Liberase TM (Roche) for 25 min at 32°C. The cell suspension was filtered by 40-µm mini strainer (PluriSelect) and stained with 1-3 µg/ml DAPI. Live single cells that were either Citrine-positive (Citrine+Cherry-), Cherry-positive (Citrine-Cherry+) or double-positive (Citrine+Cherry+) were isolated using fluorescence-activated flow cytometry (FACS) on an MA900 instrument (Sony). The Smart-seq3 protocol. Reference: M. Hagemann-Jensen et al., Single-cell RNA counting at allele and isoform resolution using Smart-seq3. Nature Biotechnology 38, 708-714 (2020)