GSM6774369: MDA-F471 CSCs replicate 3; Mus musculus; RNA-Seq
Total RNA was purified from MDA-F471 cells and the spheres (6 samples) using the RNeasy Plus Kit (Qiagen) according to the manufacturer's protocol The Ribo-zero RNA removal kit was used to remove ribosomal RNAs from total RNA samples. Paired-end libraries (101 bp reads) were prepared from 500 ng total RNA using the TruSeq stranded total RNA LT sample kit (Illumina) according to the manufacturer's instructions.