Whole transcriptome sequencing of papillary thyroid cancer of Indian origin
Whole transcriptome sequencing of papillary thyroid cancer of Indian origin
The FFPE tumor tissues were collected from ACTREC-TMC The 40 FFPE orphan tumor samples has been subjected to microtome-sectioning where ~5-6 sections of 10 um has been taken based on the tissue abundance for RNA extraction. The RNA extraction from has been performed according to standard protocol of Qiagen's RNeasy FFPE tissue kits. The RNA concentration was measured using Nanodrop 2000c spectrophotometer. Using the Illumina RNA Prep with enrichment Kit, formerly known as the TruSeq RNA Access Library Prep Kit, whole transcriptome sequencing was done on 40 FFPE samples of papillary thyroid cancer.