GSM8082904: Cardiomyocytes, EMPA_IR_3; Mus musculus; RNA-Seq
Samples were sorted into sterilized and RNAase-free polypropylene tubes, each one containing 2 mL of the RL-Lysis buffer (provided within the Norgen single-cell RNA isolation kit), and then inserted into the cell collection chamber. Cells were sorted immediately into RL-Buffer and then vortexed. QuantSeq 3' mRNA-Seq Library Prep Kit for Illumina (FWD) (Lexogen) was used, according to manufacturer's instructions.