RADcap target enrichment of  cv_aridus_MLZ_45554
We used 250 ng of DNA in 25 uL volume to prepare dual-indexed genomic libraries of each sample at  volume reactions using the KAPA Hyper Prep library preparation kit and custom indexes (Glenn et al., 2019). We combined the libraries into pools containing between six and eight samples for enrichment, and we kept tissues and toepads in separate pools. We enriched each library pool for a set of 2,351 genus-specific RAD loci (Salter et al. 2023) using a MYbaits Custom Kit from Daicel Arbor Biosciences. After enrichment, we performed 14-16 cycles of PCR recovery, removed adapter-dimers using a Qiagen GeneRead Size Selection Kit, and collected paired-end 150 bp sequence data two lanes on an Illumina NovaSeq 6000.