GSM7049669: MCF7 Vehicle Rep2; Homo sapiens; RNA-Seq
Toral RNA was extracted with TRIZOL (Invitrogen) and sonicated. 4sU RNA was labelled with biotin and biotinilated RNA was purified with µMacs Streptavidin Kit (Miltenyi). RNA-seq llibraries were prepaired using theNEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®.