GSM7977955: lungs, Wild Type C57Bl6, Rabbit IgG, rep1; Mus musculus; OTHER
100,000 EpCAM+ cells were bound to concanavalin A beads, then incubed with antibodies against PRDM16, NKX2-1, PRDM3, or H3K4me3. IgG controls with either a rabbit or sheep IgG were also generated. Cells were incubated with pAG-Mnase to facilitate anitbody-guided DNA digestion. The extracted DNA was purified by phenol-chloroform-isoamyl alcohol (Invitrogen: 15593-031) extraction and  Phasemaker tubes (Invitrogen: A33248) DNA was quantified using a Qubit (ThermoFisher) and libraries were constructed using the Illumina DNA Library Prep Kit (Cell Signaling Technology, 56795S) and Multiplexoligos (47538S). Libraries were sequenced for at least 15mil reads. Libraries sequenced by Genewiz