GSM6729758: neutrophil granulocytes, murin, Ctrl PFCs [603-50]; Mus musculus; RNA-Seq
RNA was harvested using Rneasy mini plus kit (Qiagen). The library preparation was performed according to the manufacturer's protocol using the Illumina® 'Stranded mRNA Prep, Ligation' Kit. Briefly, 25 ng total RNA were used for mRNA capturing, fragmentation, the synthesis of cDNA, adapter ligation and library amplification.