GSM7385289: 4F2A cell, DMSO, DAY2_1 [ATAC]; Mus musculus; ATAC-seq
ATAC-seqwasperformedbyHyperactiveATAC-seqLibraryPrepKitforIllumina(TD711,Vazyme).Inbrief,atotalof50000cellswerewashedtwicewith50ulofTWbufferandresuspendedin50ullysisbuffer(RSbuffer,11%NP40,10%Tween,1%Digitonin). ATAC-seq was performed by Hyperactive ATAC-seq Library Prep Kit for Illumina (TD711, Vazyme).  The suspension was then centrifuged for 10min at 500g at 4℃, followed by adding 50ul transposition reaction mix (TWbuffer, 10%Tween, 1% digitonin, ddH2O, 5*TTBL, TTE mix V50) and then incubated at 37℃ for 30min. 5μl stop buffer was added to stop reaction. DNA was isolated by ATAC DNA Extract Beads. ATAC library was subjected to 17 cycles of amplification with TruePrep Index Kit V2 for Illumina (TD202, Vazyme). ATAC library was purified by ATAC DNA Clean Beads and measured using Qbit 4 fluorometer. Library integrity was checked by gel electrophoresis. Finally, the ATAC libraries were sequenced on Illumina NovaSeq 6000 platform to generate 150 bp paired-end reads at BerryGenomics Corporation.