RNA-Seq of Mus musculus brain regions after sleep deprivations
C57Bl/6J mice, both males and females were maintained under a 12h/12h light/dark cycle and sleep deprived for 6h from lights on. Animals were sacrificed and brains isolated. Total RNA was extracted from 1mm punches of brain tissue extracted with Trizol. 100 ng of RNA was used to prepare libraries using the Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus kit following the manufacturers instructions. Following library preparation, library concentration was determined using the Library Quantification Kit for Illumina Platfroms (Roche, UK) following the manufacturers instructions. RNA-seq was performed as 150-bp paired-end sequencing on Illumina platform by Novogene (Cambridge, UK).