GSM7839366: NEVLP2, scRNAseq; Rattus norvegicus; RNA-Seq
The perfusate was made up of 65mL William's E Media (Quality Biological, Gaithersburg, MD), 3,250U each Penicillin/Streptomycin (Life Technologies Corporation, Grand Island, NY), 0.65mM sodium pyruvate (Sigma-Aldrich, St. Louis, MO), 1.30mM L-glutamine (Sigma-Aldrich), 1% human albumin (Baxter), 500U heparin, 15mg papaverine (American Regent, Inc., Shirley, NY), 1mg insulin (Sigma-Aldrich), 1.25mg hydrocortisone (Pfizer, New York, NY), and 30mL of leukoreduced, packed Lewis rat red blood cells. Tulane Cypress high performance computer was used to process pair-ended raw fastq files generated from the sequencing experiments following the cellranger pipelines (10x Genomics), with a “filtered feature bc matrix” output folder that contains matrix.mtx.gz, barcodes.tsv.gz and features.tsv.gz files.