GSM7658312: A_229E_72h; Homo sapiens; RNA-Seq
RNA was harvested using the Rneasy Mini Plus Kit (Quiagen). A total of ~1ug of RNA was used for construction of sequencing libraries. According to the manufacturers protocol (Novogene): Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries were pooled and sequenced on Illumina NovaSeq 6000, according to effective library concentration and data amount. Library preparation and sequencing was performed by Novogene (mRNA library preparation (poly A enrichment))