GSM7474720: RVL of 16 weeks WKY rat rep1; Rattus norvegicus; RNA-Seq
Brainstems were removed and frozen in OCT blocks which were then sectioned at 200um. A tissue punch was used to extract the appropriate regions. The collected tissue was then lysed and RNA-extracted using the Direct-Zol kit from Zymo to obtain total RNA. Total RNA was sent to GENEWIZ for library preparation and RNA-sequencing. Library construction performed by GENEWIZ.