GSM7839607: Cord blood fresh, EPCR+; Homo sapiens; RNA-Seq
FACS-isolated primary cells or lentiviral transduced and cultured cells harvested by pipetting were washed with PBS/0.04% ultrapure BSA, and resuspended in PBS/BSA for loading into the 10X Genomics Chromium Single Cell B Chip, and processed in the Chromium single cell controller. The 10X Genomics Version 3.1 single cell 3' kit was used to prepare single cell mRNA libraries for freshly isolated primary cells or cultured according to manufacturer protocols with the Index Kit T Set A. Sequencing was performed for pooled libraries from each sample on an Illumina NextSeq 500 using the 100 cycle, high output kit.