GSM7118555: MV411_KO_1; Homo sapiens; RNA-Seq
1x10e6 cells were harvested and 2µL of 1:100 ERCC RNA Spike in mix (Thermo Fisher) were added to each sample for normalization. RNA was isolated using the Rneasy Kit (Qiagen) including on-column DNAse digestion. RNA libraries were prepared for sequencing using standard Illumina protocols. RNA-seq, paired-end sequencing, 150 base pair poly A enrichment, RNA libraries were prepared for sequencing using standard Illumina protocols