Applicability of DNA barcoding-based analyses on native herbivores' diet studies: the diet of the gray brocket deer (Subulo gouazoubira) in xeric hillside forests of Uruguay
We performed PCR experiments to amplify a fragment of approximately 141 bp corresponding to a region of the intron of the gene trnL (UAA) of the chloroplast using the primers c (5-CGAAATCGGTAGACGCTACG-3) and h (5-CCATTGAGTCTCTGCACCTATC-3), as described by Taberlet et al. (2007). These primers had non-barcode and barcode adapter sequences to be used with Ion Torrent-PGM (Life Technologies). We used two barcode adapters (BC54 and BC57) on forward primers to distinguish two pooled samples based on the season sampling time.