GSM7711042: Sample-2, Mock_8h, scRNAseq; Homo sapiens; RNA-Seq
Caco-2 cells were washed with PBS and treated with trypsin to make a single cell suspension, then filtered through a 70uM filter. Cells were spun down and resuspended in the appropriate volume for 10x scRNA-seq. Libraries were made according to the manufacter's instructions (single cell 3' v3.1 protocol, 10x Genomics). Caco-2 cells were resuspended in the master mix and loaded with a target cell recovery of 6,000 cells together with partitioning oil and gel beads into the chip to generate the gel bead-in-emulsion (GEM). RNA was reverse transcribed into cDNA, which was cleaned using Silane DynaBeads and amplified. Fragments were cleaned using SPRIselect reagent.