GSM6620267: RNA-seq, Drosophila adults; Drosophila melanogaster; RNA-Seq
Total RNA was isolated from Drosophila adults using Quick RNA MiniPrep kit (Zymo Research). 1 microgram of total RNA was used for cDNA synthesis using Maxima H minus reverse transcriptase master mix (ThermoFisher). The single stranded cDNA was converted into double stranded cDNA by linear amplification using REPLI-g Midi kit (Qiagen). The linear amplified cDNA was cleaned using DNEasy PowerClean Pro Cleanup Kit (Qiagen). 50 ng cDNA was used to prepare the library using Illumina DNA Prep, (M) Tagmentation library preparation kit (Illumina) following the manufacturer's user guide.