GSM7474763: PBMCs, CF, p6; Homo sapiens; RNA-Seq
Human PBMC were isolated from 7 mL of blood by density gradient centrifugation and immediately processed. Single cells were encapsulated and barcoded using the 10x Chromium Controller platform (10X Genomics) and  Chromium Single Cell 3' Reagent Kit (v3.1 Chemistry) (10X Genomics). Sequencing libraries were prepared according to the manufacturer's protocol. Size distribution and concentration of resulting libraries were assessed using HS DNA assay on TapeStation platform (Agilent). Libraries were sequenced on a Novaseq 6000 (Illumina) platform aiming at 50'000 reads per single cell.