GSM7976919: KEPi18_VAL+S.2; Oryctolagus cuniculus; RNA-Seq
Total RNA was isolated using trizol/chloroform protocol followed by RNeasy mini kit (Qiagen #74106) with a DNase I (Qiagen #79254) treatment. Three nanograms of total RNA were used for amplification using the SMART-Seq V4 Ultra Low Input RNA kit (Clontech) according to the manufacturer's recommendations (10 PCR cycles were performed). cDNA quality was assessed on an Agilent Bioanalyzer 2100, using an Agilent High Sensitivity DNA Kit. Libraries were prepared from 0.15 ng cDNA using the Nextera XT Illumina library preparation kit. Libraries were pooled in equimolar proportions and sequenced (Paired-end 50–34 bp) on an Illumina NextSeq500 instrument, using a NextSeq 500 High Output 75 cycles kit.