GSM7798620: HeLa S3, shSETD3, NS, rep2; Homo sapiens; OTHER
Cells were lysed with DNAzol (Invitrogen, 10503027) at room temperature for 5 min. Genomic DNA was purified according to the manufactory's instructions. DNA was denatured by boiling 10 min at 100 °C and then loaded onto 13 mL neutral 5%-30% sucrose gradients prepared in TEN500 (10mM Tris, pH7.9, 2mM EDTA, 500mM NaCl) in ultraclear tubes. The mixture was centrifuged in SW40 rotor at 25,000 rpm for 20 h. An 1 mL-fraction was withdrawn from the top of the gradients. DNA fragments ranging from 0.5-2.5 kb were collected and purified. the VAHTSTM Universal DNA Library Prep Kit for Illumina® V3 (ND607, Vazyme)