GSM7839039: Cortex_cytoplasm_control4; Homo sapiens; miRNA-Seq
Complete details can be found on in our recent study (Vangoor et al., MedRxiv. 2021). Briefly, sub cellular fractionation was performed isolating pure cytosolic and nuclei cell compartments. Isolated cytoplasmic and nuclei samples were stored in Qiazol (Qiagen) at -80oC until RNA extraction was performed. Total RNA was extracted using miRNeasy kit (Qiagen), according to manufacturer's protocol. For small RNA library construction and sequencing, small RNAs of <200 nucleotides were purified using the miRVana microRNA isolation kit (Ambion, Life Technologies). 100 ng of small RNA was prepared for sequencing using the TruSeq Small RNA preparation kit (Illumina, San Diego, California, USA). Small RNA sequencing was performed on an Illumina HiSeq 4000 sequencer at the Beijing Genomics Institute (BGI) (Hongkong, China).