Illumina NovaSeq 6000 sequencing; RNA-seq of C57BL/6 mice splenocytes at chronic stage of Trypanosoma evansi infection
RNA-seq of C57BL/6 mice splenocytes at chronic stage of Trypanosoma evansi infection
Mice were infected by intraperitoneal (i.p) injection of with 200 parasites in 100 uL of Dulbecco's Phosphate Buffered Saline. Spleens  were isolated at day 42 p.i. for T. evansi Merzouga 93. Single cell suspensions were prepared by homogenizing spleen in 6 mL of DMEM supplemented with 10% FBS and 1% penicillin/streptomycin using gentleMACS Dissociator. Cells were spun down at 314 x G for 7 minutes at 4oC after being filtered through a 70 µm pore size cell strainer. Red blood cells were removed using RBC Lysis Buffer from Biolegend. Spleen cells suspension from a single mouse and pooled spleen cells suspension from 3 individual mice (1:1:1 ratio) were prepared for further protocols.. Samples containing 10000 cells each were carried out following 10X Genomic Chromium Single Cell 3ʹ v3 pipeline Samples were carried out following 10X Genomic Chromium Single Cell 3ʹ v3 pipeline. No feature barcoding is used.