Tbc1d10c knockout
Live CD8 T cells from Tbc1d10c+/+ and Tbc1d10c-/- spleens were captured on a microfluidic chip at approximately 5000 cells per sample and processed for single-cell RNA sequencing with the 10X Genomics Chromium 3 Solution platform. cDNA synthesized by this method was amplified and sequenced on an Illumina NovaSeq 6000