Resequencing of Rosa sp.: cultivar Old Blush
Overlapping Illumina PE libraries were prepared using a semi-automated protocol. Two independent DNA fragmentations were performed from the extracted DNA using the E210 Covaris instrument (Covaris, Inc., USA) to generate fragments mostly around 300 bp. End repair, A-tailing and Illumina compatible adaptors (BioScientific, Austin, TX, USA) ligation were performed using the SPRIWorks Library Preparation System and SPRI TE instrument (Beckmann Coulter), according to the manufacturer protocol. DNA fragments were then PCR-amplified using Platinum Pfx DNA polymerase (Invitrogen) and Illumina adapter-specific primers. Fragments of around 300 bp were size selected on 3% agarose gel. Library traces were validated on an Agilent 2100 Bioanalyzer (Agilent Technologies, USA) and quantified by qPCR using the KAPA Library Quantification Kit (Kapa Biosystems) on a MxPro instrument (Agilent Technologies, USA). The PE libraries were sequenced using 100 base-length read v3 chemistry in paired-end flow cell on the Illumina HiSeq 2000 (Illumina, USA). Fastq files correspond to PE sequences for 3 flow cells numbered: AZU_SOSW_3, AZU_SOSW_4 and AZU_SOSW_5.