GSM7475080: BL_M_47081; Macaca mulatta; RNA-Seq
Lymph node cells obtained from vaccinated animals by LN biopsy. Cells sorting were performed and CD4+ memory and naïve population were collected. RNA was extracted from CD4+ memory and Naïve cells using QIAgen kit Library construction was performed based on manufacturer's recommendation for SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio USA Inc., California, USA) followed by Nextera® XT DNA Library Prep Kit (Illumina, California, USA). Final library size was about 450bp with an insert size of about 300bp. Illumina® 8-nt dual-indices were used. Equimolar pooling of libraries was performed based on QC values and sequenced on an Illumina® NovaSeq S4 (Illumina, California, USA) with a read length configuration of 150 PE for 40 M PE reads per sample (20M in each direction)