ZOANDIET
Using the primer pairs m1COIintF and jgHCO2198 (COI), with this PCR conditions( initial step at 95C for 5 mins, followed by 16 cycles of 10 s at 95C, and a touchdown annealing of 30 s beginning at 62C until 46C; and elongation at 72C for 60 s; this was followed by 24 cycles of 10 s at 95 C, 30 s at 46C and 60 s at 72C, followed by a 10 mins final elongation at 72C). Amplicons were purified by magenetic separation and sequenced in the Illumina MiSeq sequencer using the MiSeq Reagent Kit V2 150bp x 2 (Illumina, San Diego, CA, USA).