GSM6685128: snout, Pig ID 81-8, 8 months; Sus scrofa; RNA-Seq
4mm biopsies were taken from different skin sites (flank, snout, dorsal foot and volar foot skin) and immediately put in RNA later for storage at -20℃. RNA was isolated using the Qiagen Rneasy Fibrous Tissue Mini Kit (#74704) and tissue homogenization was performed using the Qiagen Tissue Lyser II. RNA concentration was measured using Nanodrop and final quality control via visualization of 18S & 28S rRNA bands was done before sending samples to sequencing core facility. TruSeq Stranded mRNA Prep with minor modifications to the protocol for automation on liquid handling systems.