GSM7926423: Ctrl LIC-e cells ATAC-Seq Rep3; Mus musculus; ATAC-seq
50,000 LIC-e cells were collected for each sample, lysed and their nuclei were isolated. Tagmentation was performed using Nextera Tn5 transposase (Illumina Nextera ATAC seq kit, 20034197). Libraries were prepared using dual end indexing using NEBNext Ultra II DNA Library Prep Kit (E7645L) using the manufacturer's protocol, quantified using KAPA library quantification kit (Roche KK4824) and equimolar libraries were pooled. Libraries were prepared using dual end indexing with NEBNext Ultra II DNA Library Prep Kit (E7645L) using the manufacturer's protocol. Concentrations and sizes of libraries were determined using Agilent 2200 Tapestation and KAPA library quantification kit (Roche KK4824). LIbraries from all samples were pooled in equimolar concentrations and denatured along with 1% of PhiX for paired-end sequencing. Pooled library quantification was determined using  KAPA library quantification kit (Roche KK4824).