transcriptomic sequencing of mouse: CTL2
Total RNA was extracted from the right TA (n = 4 per group) of aged mice using Trizol reagent (Invitrogen, CA, USA) according to the instruction of the manufacturer. Two rounds of purification were performed using Dynabeads Oligo (dT) (Thermo Scientific, CA, USA) to purify mRNA from total RNA. Purified mRNA was fragmented under 94  for 57 min using the Magnesium RNA Fragmentation Module (NEB, cat. e6150, USA). cDNA was reverse-transcribed from cleaved RNA fragments and used to establish the cDNA library.