Chouioia cunea
Library preparation and sequencingONT-RegularA total amount of 3-4 g DNA per sample was used as input material for the ONT library preparations. After the sample was qualified, size-select of long DNA fragments were performed using the PippinHT system (Sage Science, USA). Next, the ends of DNA fragments were repaired, and A-ligation reaction were conducted with NEBNext Ultra II End Repair/dA-tailing Kit (Cat# E7546). The adapter in the SQK-LSK109 (Oxford Nanopore Technologies, UK) was used for further ligation reaction and DNA liabrary was measured by Qubit 4.0 Fluorometer (Invitrogen, USA). About 700ng DNA library was constructed and performed on a Nanopore PromethION sequencer instrument (Oxford Nanopore Technologies, UK) at the Genome Center of Grandomics (Wuhan, China).