GSM6022035: hTERT-RPE1, sh_RNA#3 for PIP4K2B; Homo sapiens; RNA-Seq
RNA extraction performed with commercial kit (ThermoFischer) For each experimental sample, totRNA was extracted using the RNeasy Prep kit (QIAGEN), and its abundance was measured using Qubit 4.0 and integrity assessed using Agilent Bioanalyzer 2100 using Nano RNA kit (RIN > 8). An indexed- fragment library was prepared starting from 500ng totalRNA using Illumina Stranded mRNA Prep ligation kit (Illumina) according to manufacturer's instructions. Indexed libraries were quantified with Qubit HS DNA kit and controlled for proper size using Agilent Bioanalyzer 2100 High Sensitivity Dna kit prior to normalization and equimolar pooling to perform a multiplexed sequencing run.