Oxford Nanopore sequencing of Vitis vinifera (Dakapo variety) leaf tissue
DNA was isolated from samples using a modified Qiagen Genomic-tip protocol. Short read elimination was performed using the Circulomics Short Read Eliminator kit. Barcoded sequencing libraries were then prepared using the Oxford Nanopore Technologies Ligation Sequencing Kit 1D (SQK-LSK109) and Native Barcoding Expansion Kit (EXP-NBD104). The pooled libraries were then loaded on a PromethION FLO-PRO002 (R9.4.1) flow cell and sequenced on a PromethION24, running MinKNOW Release 21.11.7, to 17-28.5X coverage. Base calling and demultiplexing were done using Oxford Nanopores Guppy (v5.1.13) with the High Accuracy base calling model.