GSM5908216: Input-C; Escherichia coli PCN033; OTHER
Bacterial genomic DNA of input libraries and output libraries recovered from mouse brain, spleen, and lung were extracted from using E.Z.N.A.® Bacterial DNA Kit (Omega Bio-Tek, USA) The genomic DNA was subjected to fragmentation, end repair, and 3' end dA-tailing. The Illumina universal adaptors were added using the TIANSeq Quick Connect Module (Cat# NG303, TIANGEN Biotech, China) which was then purified using Magic DNA Select Beads (Cat# M3022, Magic Bio, China). Primer pair F-/R- was used in the first round PCR amplification for enrichment of the DNA region flaking the transposon. The PCR product was purified and used as the template for the second round amplification using P5/7 primer pair, and the amplification product was purified which was used to construct Illumina sequencing library by using Magic DNA Select Beads (Cat# M3022, Magic Bio, China). The DNA library was sequenced using an Illumina Novaseq sequencer.