GSM6025448: a249; Mus musculus; RNA-Seq
RNA preparation was carried out using the RNeasy Plus Mini Kit (QIAGEN) according to the manufacturer's protocol Quantity of total RNA was assessed with the Qubit 2.0 and quality was checked using a RNA 6000 Pico chip on Agilent's bioanalyzer. Samples with an RNA integrity number (RIN) of > 8 were used for library preparation. 1 ng of total RNA was used for cDNA synthesis and amplification with Takarabio's SmartSeqv4 kit. 1 ng of the resulting cDNA was used for library preparation with the Nextera XT DNA Sample Prep Kit according to the manufacturer's instructions (Illumina). Library concentration was determined by Qubit2.0 system and library size distribution was assessed using a High Sensitivity DNA chip (Agilent) on a Bioanalyzer 2100. Barcoded RNA-Seq libraries were sequenced (150 PE) at Novogene (Cambridge, UK) on an Illumina NovaSeq.