NextSeq 550 paired end sequencing; TRAP-seq of mouse astrocytes involved in central nervous system remyelination
TRAP-seq of mouse astrocytes involved in central nervous system remyelination
Corpus callosi were dissected from LPC-injected and non-lesioned mice and were processed for TRAP. 2-3 month-old mice were anaesthetised with isoflurane and demyelinating lesions were induced by stereotaxically injecting 2 µl 1% lysolecithin (LPC; v/v) diluted in phosphate-buffered saline (PBS) into the corpus callosum. RNA was extracted from TRAP samples using the Agilent Nanoprep kit. For Library preparation, the SMART-Seq v4 Ultra Low input RNA kit (Takara Bio USA) was used according to manufacturer's instructions