GSM7049370: THP, H3K27ac, rep 2; Homo sapiens; OTHER
Adherent cells were detached with Accutase and processed using the EpiCypher CUTANA CUT&RUN kit according to manufacturer instructions. 0.5 ng E. coli spike-in DNA was added for normalization. CUT&RUN libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs). Libraries were size selected using Ampure XP bead from Beckman Coulter to retain fragments >150 bp. Libraries were amplified using 14 cycles on the thermocycler and validated using the Agilent High Sensitivity D1000 Tapestation Kit. All libraries were pooled and sequenced on a 150 cycle Mid Output v2.5 Illumina kit.