GSM6620257: TIL,Responder2,12h coculture [R2_2357_CD69pos]; Homo sapiens; RNA-Seq
The cells were harvested, stained, and sorted using FACS Aria Fusion Cell Sorter (BD Bioscience). T cells CD3+CD8+CD69+ and CD3+CD8-CD69- were sorted directly into lysis buffer, and RNA was isolated using an RNA isolation kit (Macherey-Nagel, RNA-XS). cDNA libraries were synthesized using the commercially available SMART-Seq Ultra Low Input RNA kit (Clontech). cDNA libraries were first tagmented and then barcoded by indexing primers using the Nextra XT kit (Illumina)