GSM6590595: OA3.Ts cells, mock-infected [miRNA 1]; Ovis aries; miRNA-Seq
Total RNA from each sample was extracted with TRIzol reagent (Invitrogen, USA). Approximately 1 μg of total RNA were used to prepare miRNA library according to protocol of TruSeq small RNA Sample Prep Kits (Illumina, USA). Briefly, the miRNA molecules were ligated to a 5' adaptor and a 3' adaptor by T4 RNA ligase (Promega, USA). Subsequently, the adaptor-ligated miRNAs were reverse transcribed into cDNA and PCR amplified. PCR products were purified and DNA fragments of 150±10 bp (the length of small RNA inserts plus the 5' and 3' adaptors) were quantified following the Solexa sequencing protocol (Illumina).