GSM8083024: Hoxa11CreER Hif1a, replicate 3; Mus musculus; RNA-Seq
Burn and tenotomy was performed as previously described (PMID 26274052) in Hoxa11CreER;ROSA-LSL-TdTomato;Hif1a fl/fl. Tenotomy injury site was harvested from Day 7 post-tenotomy mice. Tissue samples were digested for 45 minutes in 750U/ml Type 1 Collagenase and 7U/ml Dispase II (Gibco) in Roswell Park Memorial Institute (RPMI) medium at 37°C under constant agitation of 160rpm. Digestions were subsequently quenched with 2% FBS in PBS and filtered through 40?m sterile strainers. Cells were then washed in 2% FBS in PBS, counted and resuspended at a concentration of 1000-1200 cells/ul. Cell viability was assessed with Trypan blue exclusion on a Countess II (Thermo Fisher Scientific) automated counter and only samples with >85% viability were used for scRNA sequencing. Single Cell 3' v3