Metagenome from wastewater: sugar production plant after modernization technical replicate 3
DNA quality and template concentration were determined using a Qubit fluorometer (Thermo Fisher Scientific, Waltham, USA), and DNA integrity was analysed by 1% agarose gel electrophoresis and by PFGE using BioRad CHEF-III instrument (BioRad, Hercules, USA). The long reads were generated using the MinION sequencer (Oxford Nanopore Technologies, Oxford, UK). Prior to long-read library preparation, genomic DNA was size selected on the Bluepippin instrument (Sage Science, Beverly, USA). DNA fragments longer than 10 kbp were recovered using a PAC30kb cassette. 5 g of recovered DNA was used for 1D library construction using SQK-LSK109 kit according to the manufacturers instructions and 0,5 g of the final library was loaded onto the R9.4.1 flow cell and sequenced on a MinION sequencer.