GSM7977584: Somatic_yki RNAi_Early_1; Drosophila melanogaster; RNA-Seq
sorted cells in Trizol were processed through Zymo RNA Micro-Prep kit (Zymo Research R2060) for RNA extraction. Poly A selection was done using Takara PrepX mRNA-8 (Takara 640098) protocol. cDNA libraries were prepared with Takara PrepX mRNA-8 (Takara 640096) protocol. Libraries were amplified for 15 cycles in PCR with sample specific PrepX Index primers and sequenced in 50bp single end runs on a Illumina HiSeq 2500.