GSM6813162: 5029_6h_0.1_ng_ml_tnfa; Homo sapiens; RNA-Seq
Total RNA was isolated using the RNeasy Mini Kit (74106, Qiagen), including an on-column DNase digestion (79254, Qiagen) Total RNA samples having RIN values>8 were subjected to TruSeq stranded mRNA library preparation, according to the manufacturer's instructions (Document # 1000000040498, Illumina) Stranded mRNA libraries were analyzed on a 2100 Bioanalyzer instrument, following the manufacturer's protocol “Agilent DNA 7500 kit” (G2938-90024, Agilent Technologies), diluted to 10nM and pooled equimolar into multiplex sequencing pools for sequencing on HiSeq 2500 instrument (Illumina).