GSM6813018: S602_H3K4me1_1; Homo sapiens; ChIP-Seq
SF10602 cells were collected and suspended in digestion buffer. Digestion was performed using microccocal nuclease to obtain 150 pb DNA fragments. Histone-DNA complexes were isolated with the desired antibody. The ChIP-Seq libraries are prepared using the Swift ACCEL-NGS 2S Plus DNA Library Kit, with dual indexing. We used 5.4ng dna/library, and 7 cycles for the final PCR library amplification step. Quality control is performed on each Input/IP libraries using Qubit and Agilent TapeStation HS D1000. Libraries were quantitated using the KAPA Illumina Library Quantitation kit, pooled, and sent to the UM Advanced Genomics Core for PE-50 sequencing on Illumina's NovaSeq6000