GSM6620035: Mef2c_V2; Mus musculus; ChIP-Seq
Homogenized tissues were crosslinked in fresh 1% formaldehyde/PBS for 30 min, quenched with 500 mM glycine. Crosslinked nuclei were sonicated using a QSonica 700. Sonicated chromatin was centrifuged, and the supernatant used for chromatin immunoprecipitation (ChIP) with Invitrogen M280 Streptavidin DynaBeads (Life Technologies). DNA was RNase A and Proteinase K treated the following day and purified using Qiagen MinElute (cat. 28004) for subsequent NGS library synthesis. Libraries for bioChIP DNA and corresponding input samples were synthesized using KAPA Hyper Prep library kit (#KK8502). Each library was generated according to manufacturer's protocol.