Shotgun sequencing AD microbiome: R-mag after sulfate shock
Library strategy: metagenomics. Genomic DNA was isolated and purified using the DNeasy PowerSoil (QIAGEN 181 GmbH, Hilden, Germany). Genomic DNA quality and quantity were determined using Multiskan Sky Microplate Spectrophotometer (operated with Thermo Scientific SkanIt Software 5.0, ThermoFisher Scientific) and Qubit fluorometer (Life Technologies, Carlsbad, CA). Illumina libraries were prepared with the Nextera DNA Flex Library Prep kit (Illumina Inc., San Diego CA) and sequenced on the Illumina NovaSeq platform producing 150 bp paired-end reads