GSM8002888: gWAT, HFDSG2; Mus musculus; RNA-Seq
Total RNA was extracted from mouse gWAT by using Maxwell® RSC 48 Instrument(Promega) and Maxwell® RSC simplyRNA Tissue Kit (Promega, AS1340) in accordance with manufacturer's instruction. The complemenary DNA libraries were generated using the TruSeq Stranded mRNA Library Prep Kit (Illumina). The quality of total RNA and cDNA were assessed using the Agilent 2100 Bioanalyzer with the RNA6000 nano Kit and the DNA7500 kit (Agilent Technologies). Sequencing was performed using the NovaSeq6000 system (Illumina) with a pair-end-read sequencing length of 150bp.