GSM7049854: left ventricular, ZSHLF, rep 3; Rattus norvegicus; miRNA-Seq
Total RNA was extracted from the left ventricular using TRI Reagent (Sigma T9429) and Nanodrop 1000 was used for RNA quantification and quality assurance. RNA samples from different groups were mixed for subsequent cDNA library construction. The library construction process mainly includes the following steps: connecting the 3′ end and the 5′ linker, reverse transcription into cDNA, and then performing PCR amplification, size selection of 135-155 bp PCR amplified fragments. DNA fragments in libraries were denatured with 0.1 M NaOH to yield single-stranded DNA.