GSM7385246: ChIPseq_48hDox_3hH2O_Pol2CST_BRD4G3-TetOEGFP; Homo sapiens; ChIP-Seq
ChIP-seq: DLD1 cells were crosslinked with 15ml 1% PFA (ThermoFisher, #28908) in PBS on 15cm plates for 10 minutes at RT and quenched by the addition of 2ml 2.5M glycine followed by shaking for 5 minutes. Crosslinked cells were collected by scraping. Chromatin sonication was performed for 10 minutes using a Covaris E200 set to 10% duty factor, 200 cycles per burst, and 140 peak intensity power. ChIP-seq: Eluted DNA was purified with the QIAquick PCR Purification Kit (Qiagen, #28106), and libraries were prepared using the KAPA HTP library preparation kit (Roche, #07961901001) for sequencing on the NovaSeq 6000 RNA-seq: Total RNAs from 6-well plate were extracted using the RNeasy mini kit from Qiagen (Qiagen, #74106). RNA-seq: mRNAs were enriched using a NEBNext poly(A) mRNA magnetic isolation module (NEB, #E7490L). RNA libraries were prepared using NEBNext Ultra II Directional RNA library preparation kit (NEB, #E7760L) and sequenced on the Illumina NovaSeq 6000.