RNA-seq of p53_WT_MSC_3
cDNA was synthesized using a SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech, Palo Alto, CA, USA). ds-cDNA was fragmented using a S220 focused ultrasonicator (Covaris) and cDNA libraries were then generated using a NEBNext Ultra DNA Library Prep Kit (New England BioLabs, Beverly, MA, USA).