GSM6591748: sox4; Mus musculus; ATAC-seq
Cells were collected and lysed to obtain purified nucleus; The Tn5 enzyme transposable reaction was carried out and the open regions of chromatin were fragmented; the fragmented DNA was purified; the purified DNA was amplified. The amplified DNA was sized to remove non-target fragments. ATAC libraries were prepared for sequencing using TruePrepTM DNA Library Prep Kit V2 for Illumina kit.Purified fragmented DNA were amplified by PCR reaction,and the libraries were first purified by MinElute PCR purification column(Qiagen)and then further size-selected by VAHTS DNA Clean Beads to obtain libraries with a size distribution between 100 and 1500 bp.