16S Sequencing of water metagenome: Quincy downstream
For 16S rRNA gene PCR amplification, 100 ng of genomic DNA was added as template into a total reaction volume of 25 L. Dual-indexing strategy (Kozich et al., 2013) and a universal primer set 515F (5 - GTGCCAGCMGCCGCGGTAA )/806R(5 -GGACTACHVGGGTWTCTAAT-3) targeting the V4-V5 region of both bacterial and archaeal 16S rRNA gene was amplified