RNAseq of Rhipicephalus zambeziensis salivary glands using PacBio Isoseq protocol
Adult females of R. zambeziensis feeding on a Hereford (n=5 ticks, 10 salivary glands for each time point) had their salivary glands dissected at 0, 6, 12, 18, 24, 36, 48, 72, 96 and 108 hours and transferred to 1.0 ml RNAlater Solution (Invitrogen, Lithuania). Salivary glands were pooled together and the vials were sent to NovoGene (Beijing) for RNA extraction and sequencing. PolyA mRNA was used to construct both the 150 nt paired end Illumina NovaSeq and the Iso-Seq PacBio libraries.