GSM5750837: Islet 5 mock; Homo sapiens; RNA-Seq
Total RNA was extracted using Trizol. Ribosomal RNA was depleted using Ribo Zero Gold kit (Illumina). Each rRNA depleted sample was then split into two, with one aliquot digested with RNase R and the other aliquot mock digested according to Pandey et al. 2019 PE libraries were constructed using TruSeq Stranded Illumina Total RNA Preparation Kit