GSM7658441: SbGhd7, Myc, Sigma, Rep 1; Sorghum bicolor; ChIP-Seq
Cells were fixed with 1% formaldehyde for 8 minutes. After quenching and cell lysis, chromatin was sonicated using a Bioruptor Plus sonicator (Diagenode). ChIP-seq libraries were prepared using the ThruPLEX DNA-Seq Kit complemented with DNA Unique Dual Index Kit (Takara). 50-100 pg of DNA was used as starting material for input and ip samples. Libraries were amplified using 18 cycles on the thermocycler. Post amplification libraries were size selected at 200-500bp in length using agarose gel. Libraries were validated using the Agilent High Sensitivity DNA Kit.