GSM7711016: Memory CD4+ T cells CD45RO+CCR7lowCCR4+CRTH2+CCR6-, filarial infected - ADT; Homo sapiens; RNA-Seq
Multiomic single cell immune profiling was performed in three subsets of memory CD4+ T cells enriched among filarial-infected patients. Briefly, from PBMCs of ten filarial-infected patients (four HDM-Filarial+ and six HDM+Filarial+) and ten healthy donor volunteers (five HDM+Filarial- and five HDM-Filarial-), three subsets of effector memory (CCR7-CD45RO+) CD4+ T cells were sorted out, including CD4+ T cells expressing CCR4+CCR6+CRTH2- (subset 1), CCR4+CCR6-CRTH2+ (subset 2), and CCR4+CCR6+CRTH2+ (subset 3). Approximately 1 x 104 sorted cells of each subset from all patients were hash-tagged separately with TotalSeq™-C anti-human hashtag (Biolegend). After that, each patient's cells subsets were pooled together accordingly for a total of 10,000 cells per sample, generating a total of 6 samples. Approximately 1 x 104 multiplexed cells (a thousand cells per patient for each sorted subset) were stained with TotalSeq™-C Human Universal Cocktail (Biolegend) and then loaded into the Chromium Next GEM Chip G (10x Genomics) respectively. In total, 18 libraries were made in accordance with the Chromium Single Cell V(D)J Reagent Kits User Guide (v1.1) with Feature Barcoding technology, including 6 GEX libraries, 6 VDJ-enriched libraries and 6 Cell Surface Protein libraries.