RNA-seq of Mus musculus puppies brain infected with ZIKA virus
For library construction, 1 ug of high-quality RNA and the TruSeq Stranded Total RNA LT Sample Prep Kit (Illumina Inc) were applied. Firstly, the RiboZero technology (Illumina Inc.) was used to remove rRNA and preserve only poly(A) and other non-poly(A) transcripts, which were then fragmented. Quantification was performed through qPCR with KAPA Library Quantification Kit and samples were sequenced with kit NextSeq 500/550 High Output v2, paired-end 150 cycles.