Illumina Multiplexed Amplicon NGS
IDT's rhAmpSeq Design Tool for CRISPR gene editing analysis was used to design multiplexed primer pools. Libraries were generated using each primer pool and IDT's rhAmpSeq CRISPR Library Kit along with rhAmpSeq Index Primers. Samples were run according to manufacturers instructions.  Forward and reverse reads were merged with SeqPrep.  Reads were then demultiplexed and paired followed by application of quality and amplicon filters. Fastqs from all amplicons associated within each sample were combined into one fastq for SRA upload.