GSM7658332: mESCs, MTX, 12 hours, rep1 (IP); Mus musculus; ChIP-Seq
Sv/129 mouse embryonic stem cells (mESCs) were cultured under normal folate medium for at least 2 passages before 1uM methotrexate(MTX) was applied for 24hours. 10^7 mESC cells from normal folate group and MTX group were used to obtain DNA with a concentration of 1 ng / ul and fragments were digested into100-500bp for CHIP(chromatin immunoprecipitation) sequencing. The ChIP-seq library is constructed from ChIP DNA and control DNA (from an input normal IgG). Construct the libraries according to the manufacturers' instructions. Perform purification and size selection of the library by Agencourt AMPure XP according to the manufacturer's instruction. Integrity of the constructed libraries (size distribution and purity) is examined by an Agilent 2100 Bioanalyzer with a High Sensitivity DNA Kit