GSM6619532: H3K27ac_Aged_Rep1; Mus musculus; ChIP-Seq
NSCs were fixed with 1% formaldehyde for 10 min and quenched with glycine solution for 20 min at room temperature. The fixed NSCs were lysed in ChIP lysis buffer on ice. Chromatin was released during cell lysis and sonicated using a E220 focused-ultrasonicator (Covaris). ChIP-Seq libraries were prepared based on the protocol of KAPA HyperPrep Kit (Roche) complemented with the NEXTflex DNA Barcodes (Bioo Scientific).