RNA-Seq of Mus musculus: adult lateral septum
Tissue was pooled from both hemispheres of the LS from 2 mice of the same sex. Tissue was homogenized in chilled nuclei EZ lysis buffer (Millipore Sigma #NUC101) using glass dounce with ~15 strokes per pestle. Following filtration and washes, nuclei were labeled with propidium iodide (PI, ThermoFisher, 1:500), then 9000 PI+ nuclei were sorted on a Bio-Rad S3e Cell Sorter. The 10x Chromium process was performed to prepare libraries according to the manufacturer protocols (10x Genomics) and sequenced on an Illumina NovaSeq 6000.