16s RNA-Seq of Mangrove Sediment Bacteria from Wallacea
Total genomic DNA was extracted from 0.7g of surface and depth sediment using PowerSoil DNA Kits (Qiagen Inc). The V3-4 region of 16S gene was amplified using the universal primers 341f and 785r. Libraries were sequenced and paired ends analysed using UPARSE v8 and QIIME v1.9 to form operational taxanomic units of 97% sequence similarity.