GSM7839369: SAM24402082_scRNA-seq_BRM_dox; Homo sapiens; RNA-Seq
Cultured cells were trypsinized into single cell suspensions, counted using the Vi-CELL XR cell counter (Beckman Coulter) and were then processed using the Chromium Single Cell Gene Expression Reagent Kit v3.1 (10x Genomics, Cat. No. 1000121, Single Index kit) as per the manufacturer's recommendations. Library was constructed according to the manufacturer's instructions (Single Cell Gene Expression Reagent Kit v3.1, 10x Genomics, Cat. No. 1000121, Single Index kit). Libraries were profiled using the Tapestation High Sensitivity DNA kit (Agilent Technologies) and quantified with the Kapa Library Quantification Kit (Kapa Biosystems).