GSM8047906: rim101∆_pH7_5_biological_replicate_2; Malassezia sympodialis; RNA-Seq
Cultures were harvested by centrifugation at 4000 rpm for 10 min at 4˚C, then washed once with cold sterile H2O. Cell pellets were flash frozen in a dry ice-ethanol bath and lyophilized overnight. RNA was extracted from the lyophilized pellets via a Trizol-chloroform extraction (Invitrogen). The resulting RNA was treated with DNase I (New England Biolabs) for 30 min at 37˚C as described, then re-purified using Trizol and chloroform. An mRNA library was prepared using a Kapa Stranded mRNA-Seq library prep kit. Stranded mRNA-Seq was performed on an Illumina HiSeq 4000 with 50-bp single-end reads.