GSM6321085: Embryoid body, Day 3, control; Mus musculus; RNA-Seq
RNeasy Mini Kits (74104; QIAGEN, Hilden, German) were used for total RNA extraction according to the manufacturer's instructions. Extracted RNA was incubated with DNase I (2270B; Takara, Shiga, Japan) to degrade the genomic DNA. The quality of RNA was initially assessed by electrophoresis on a 1.5% agarose gel, and subsequently by absorption spectrophotometry. RNA libraries for RNA-seq were prepared using SMART-Seq v4 Ultra Low Input Kit following manufacturer's protocols.