GSM8002548: scATAC-seq, Neural organoid, Line 409b2, Day 90 Chr-Veh, Replicate 2; Homo sapiens; ATAC-seq
Single cells were dissociated using StemPro Accutase Cell Dissociation Reagent (Life Technologies), filtered through 30uM and 20uM filters (Miltenyi Biotec) and cleaned of debris using a Percoll (Sigma, P1644) gradient. Single cells were resuspended in ice-cold Phosphate-Buffered Saline (PBS) supplemented with 0.04 % Bovine Serum Albumin and prepared for single-cell separation. Subsequent nuclei preparation and scATACseq library generation was performed using the Chromium Single Cell ATAC Library & Gel Bead Kit (16 rxns PN-1000110) according to the manufacturer's recommendations without any modifications (10x Genomics). Sequencing of the 10x Genomics single-cell ATAC-seq libraries was performed on an Illumina NovaSeq 6000 (Illumina, San Diego, CA) at by the sequencing core facility at the Max Planck Institute for Molecular Genetics (Berlin, Germany).