GSM6932581: Wfdc15aRST_KO2; Mus musculus; RNA-Seq
FACS-sorted round spermatids were lysed in 2.5 μl of RLT plus buffer (Qiagen) supplemented with 0.06 μl SUPERase-In RNase inhibitor (Thermo Fisher). The samples were directly processed according to the Smart-seq2 protocol with 15 cycles of PCR. The amplified cDNA (200 pg) was used for Tagmentation reaction using Nextera XT DNA Sample Preparation Kit (Illumina) with dual index primers from Nextera XT Index Kit v2 (Illumina) according to manufacturer's instructions.