Hi-C of Thielaviopsis punctulata DSM102798
Three HiC libraries were prepared in a similar manner as described previously(Erez Lieberman-Aiden et al., 2009). Briefly, for each library, chromatin was fixed inplace with formaldehyde in the nucleus and then extracted. Fixed chromatin was digestedwith DpnII, the 5 overhangs filled in with biotinylated nucleotides, and then free bluntends were ligated. After ligation, crosslinks were reversed and the DNA purified fromprotein. Purified DNA was treated to remove biotin that was not internal to ligatedfragments. DNA was then sheared to ~350 bp mean fragment size and sequencinglibraries were generated using NEB Next Ultra enzymes and Illumina-compatibleadapters. Biotin-containing fragments were isolated using streptavidin beads before PCRenrichment of each library. The libraries were sequenced on an Illumina HiSeq X