GSM6729435: pCCL-L-ELK1-GFP transduced CD34+CD123+CD38+CD371– HSPCs derived from cord blood CD34+HSPCs [L-ELK1_CD371N_HSPC_rep2]; Homo sapiens; RNA-Seq
Two thousand of pCCL-L-ELK1-GFP, pCCL-S-ELK1-GFP, pCCL-GFP lentiviral transduced GFP+ CD34+CD123+CD38+CD371– HSPCs are sorted into 1.5 mL tube containing 200 μL 0.5% BSA-DPBS solution. The dsDNA and libraries are generated as previously described (PMID: 20203668). DNA libraries are constructed using Vazyme TruePrepTM DNA library Prep Kit V2 from Illumina (TD-503) as previous reported (PMID: 29434353,32640500, 27720903,32725842) and sequenced using theIllumina NovaSeq 6000 system (Novogene Co., Ltd.).