GSM5603821: NPC sc-seq Nrg4 KO-1; Mus musculus; RNA-Seq
Perfusion collagenase and pronase (0.4mg/ml) from portal vein into mouse liver was performed after euthanasia. Hepatocyte was removed from total cell suspension by gradient centrifugation. The NPCs were suspended in 1ml 10% FBS DMEM. RNA libraries were prepared for sequencing using standard Illumina and 10X genomics Chromium single cell protocols