GSM7089335: MPNST, PRC2 loss, biological replicate 2 [PECN7_LSN]; Mus musculus; RNA-Seq
1 mL Trizol reagent was added per tumor followed by homegenization using Tissue Ruptor II(QIAgen). Then phenol/chloroform separation and subsequent steps as outlined by Trizol reagent manufacturer (Thermo Fisher Scientific). Poly-A mRNA was purified followed by fragmentation, and first and second strand cDNA synthesis with Illumina mRNA TruSeq library preparation protocol. Double stranded cDNAs were processed for TruSeq dual-index Illumina library preparation.