Construct PTS38; transformation event 8; chloroplast-expressed sense RNA targeting PDS tobacco gene; chloroplast-enriched RNA; sRNA raw reads; biological replicate 4
Chloroplast were isolated from 250 mg of tobacco seedling leaves using the MinuteTM Chloroplast prior to isolate RNA; sRNA libraries were constructed using the RealSeq-AC from Somagenics using 150 ng of total RNA and amplified with 16 PCR cycles. Sequencing was done with the Illumina NextSeq 550.