IPOD genomic footprinting of E. coli in M9 medium, replicate 2
Genomic footprinting samples, following IPOD-HR method. After cross-linking, cell lysis and DNase digestion, a fraction of the samples was collected to perform interphase extraction of protein-DNA complexes. After interphase extraction,cross-links were reversed, DNA purified and libraries prepared using the NEBNext Ultra II DNA kit.