GSM6022347: microgila BV2 cells-BAK1-KD-Control_rep1; Mus musculus; RNA-Seq
Microglia were seeded in a 6-well plate, and after 12h for complete cell wall adhesion, Oxy-Hb stimulated the cells and reached the scheduled stimulation time. Cellular RNA was extracted using RNAeasy Isolation Reagent (#R701-02, Vazyme Biotech Co., Ltd) according to the manufacturer's instructions. The Universal V6 RNA-seq Library Prep Kit for Illumina (#NR604-02, Vazyme Biotech Co., Ltd) was used for library preparation. Briefly, the samples were normalized at a 500 ng/ml concentration. First, samples were subjected to a tagmentation redaction, indexed, and PCR amplified. Libraries were then purified with VAHTS DNA Clean Beads (#N411-02, Vazyme Biotech Co., Ltd). QC checks for the library preparations included electrophoresis (Agilent Technologies 2100 Bioanalyzer) or library quantification.