RADseq of Galeocerdo cuvier
Double-digest restriction-associated DNA (ddRAD) libraries were prepared using EcoRI and MspI restriction enzymes, a 400-bp size selection, and a combination of two indexes and 24 barcodes to pool 48 individuals per lane. The genomic libraries obtained were sequenced with a HiSeq 2500 Illumina sequencer (single-end, 125 bp).