IsoSeq barcoded libraries were prepared using the SMRTbell Express Template Prep kit 2.0. Individual libraries were barcoded using the Iso-Seq Express Oligo kit. Barcoded libraries were multiplexed in equimolar amounts into a final SMRTbell template, which was purified using 1X beads. Libraries were sequenced on the Sequel II system for 20 hours using the Sequel II Binding kit 2.1. The final loading concentration was 80 pM.