GSM7711742: BEC_12mos_MA10_4DPI_1; Mus musculus; RNA-Seq
Brains were digested with papain, microvessels were isolated by differential centrifugation through 25%BSA gradient, microvessel fragments were digested to single cells with Collagenase, myelin was depleted, and cell suspension passed three times through column based CD31-positive selection to isolate endothelial cells. RNA was extracted from purified brain endothelial cells using micro RNEasy kit (Qiagen). 1.5-3 ng of purified brain EC RNA was collected from each sample. Strand-specific RNA-SEQ libraries were prepared using an TruSEQ mRNA-SEQ library protocol