GSM7926142: lgr5, rep1; Mus musculus; RNA-Seq
Proximal mouse intestine was harvested and epithelial cells were eluted with 20 mM EDTA + 1 mM DTT and filtered through 100 and 40 um cell strainers. Filtered cells were sorted on Sony MA900 and XDP100 machines for cells positive for reporters/antibodies. Sorted cells were flash frozen and RNA was isolated using  miRNeasy Micro kit (Qiagen). Libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina. Post amplification libraries were purified using Agencourt AMPure XP beads from Beckman Coulter. Libraries were validated using Agilent High Sensitivity DNA kit.