GSM7658557: acidic_rep2; Homo sapiens; RNA-Seq
Total RNA was isolated from cells using TRIzol reagent, according to the manufacturer's instructions. rRNA-depleted RNA was used for the library preparation. The libraries were prepared with the NEBNext Ultra II Directional RNA library prep kit for Illumina (New England Biolabs) according to the manufacturer's instructions. Libraries were paired-end sequenced (2*76 base pairs) on a NextSeq500 device (Illumina), with a read depth of 20 million.