GSM5908230: BMSC-derived exosomes, Sham 2; Rattus norvegicus; miRNA-Seq
Total RNA was extracted from BMSC-derived exosomes of OVX and Sham rats using TRIzol (Thermo Fisher Scientific, Waltham, MA, USA). The integrity of total RNAs was analyzed by Agilent 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), and the RNA integrity number (RIN) > 7 was used for NGS. The concentration of total RNAs was detected by NanoDrop™ 2000. MiRNA sequencing was performed on the Illumina Hiseq2500 platform from LC Sciences (Hangzhou, China). The raw reads of each sample were internally programmed by ACGT101-miR, and clean reads were obtained by removing the adapter dimers, junk, low complexity, common RNA families (rRNA, tRNA, snRNA, and snoRNA), and repetition. Then, unique sequences of 18–26 nucleotides were mapped to the precursors of Rattus norvegicus in miRBase 22.0 to identify the known and novel miRNAs. L/R ± n indicated that the detected miRNA sequence was more/fewer n bases than the known miRNA on the left/right side. The read counts to tags per million counts (TPM) ratio was used to normalize the expression levels of miRNAs. The differentially expressed exosomal miRNAs (DE-miRNAs) were identified by fold-change ≥2 or fold-change ≤0.5 and P≤0.05.