GSM6893811: Cortical Sham 3; Mus musculus; miRNA-Seq
Muscle and connective tissue were removed from the femurs, followed by removal of the epiphyses. Marrow was removed by centrifugation. Trabecular bone was isolated using a 1mm biopsy punch and then submerged in trizol. Cortical bone was trimmed and each end and the mid diaphysis was placed in a separate trizol containing microcentrifuge tube. Bone tissues were then homogenized using an ULTRA-TURRAX homogenizer and RNA was was extracted using the Qiagen miRNeasy kit. Prior to sequencing, equal amounts of cortical or trabecular RNA from two individual mice were pooled to generate 3 replicate samples for each bone compartment and treatment group. NEBNext Multiplex Small RNA Library Prep Kit