GSM8083869: Hy_A_36; Urosaurus graciosus; Urosaurus ornatus; RNA-Seq
Liver tissue was homogenized in  TRIzol  using a Dounce glass homogenizer. After 5 min incubation at room temperature, chloroform was added. This was then shaken for 15 sec and incubated for 2-3min at room temperature. The homogenate was then centrifuged at 12,000g at 4oC for 15 minutes. RNeasy columns (Quigan) were then used following the manufacturers protocol Illumina directional RNA library preparation was done by NC State Genomic Sciences Laboratory