GSM6893833: Nectary secretory replicate 1; Melianthus elongatus; RNA-Seq
RNA was isolated from pre-secretory, secretory, and post-secretory nectaries (stages described above), as well as leaves, with the RNAqueous Micro Kit (Invitrogen cat. no. AM1931). The total RNA was treated with the TURBO DNA-free kit (Invitrogen Cat# AM1907) according to the manufacturer's protocol. Barcoded library creation and sequencing was performed via NovaSeq 6000 using via paired-end 150 bp runs using rapid chemistry