GSM7049167: i3Neuron, BORCS5-KO axon, biological rep 3; Homo sapiens; RNA-Seq
RNA from three axonal or neuronal compartments was extracted using a total of 100 μl of the kit's extraction buffer. No carrier RNA was used for the extraction. On-column DNase digestion was performed. The RNA was eluted from the columns with 10 μl RNase-free water. 5 μl for axon samples and 1 μl for neuron samples were used for synthesizing cDNAs using the SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (Takara). Nextera XT DNA Library Preparation Kit (Illumina) was used to prepare libraries from the cDNA samples using 1 ng cDNA. Libraries were run on the NovaSeq (SP Kit v1.5 – 200 cycles). Approximately 1 billion 2x100 bp reads were obtained with sequencing.