RNA-seq of Homo spaiens: 46,XY skin fibroblast; CRISPRi ZFY knockdown
Cells were collected and frozen in Qiagen RNA Protect Cell Reagent until RNA extraction with the RNeasy Plus Mini Kit which included a genomic DNA removal step with a gDNA Eliminator Column. 10ul of a 1 to 100 dilution of ERCC Spike-Ins were added per 1 million cells.  Libraries were prepared using the Roche Kapa mRNA HyperPrep Kit v2 according to manufacturers instructions. Libraries were size-selected using 2 percent agarose gels on the Sage Science PippinHT system with a capture window of 300-600 bases.