GSM6620210: Mutagenesis_MPRA_DNA1; synthetic construct; OTHER
RNA was extracted using Trizol and purified with RNA Clean and Concentrator kit from Zymo; AAV genome was released by incubating with Proteinase K solution and then used as template for MPRA library construction. DNA/RNA were reversed transcripted with UMI-primer which recognized the cloning sites of the specific enhancers. The P5 and P7 adapters were added through two cycles of touch-down PCR.