GSM7711255: Sp_5dpf_1_scRNAseq; Strongylocentrotus purpuratus; RNA-Seq
Larvae were collected, seawater was removed, and larvae were resuspended in Ca2+ Mg2+-free artificial sea water and then passed to dissociation buffer containing 1 M glycine and 0.02 M EDTA in Ca2+ Mg2+-free artificial sea water. Larvae were incubated for 10 min on ice and dissociated gently into cells via pipette aspiration. Single cell cDNA libraries were prepared using the Chromium Single Cell 3' Reagent Kit, Chemistry v3. Libraries were indexed, multiplexed and sequenced on Illumina NextSeq 500 to generate paired end reads.