GSM6620325: extracellular vesicle from wjMSC-3; Homo sapiens; miRNA-Seq
Total RNA was extracted from MSCs using TRIzol or MSC-derived EVs using TRIzol LS reagents. For RNAseq, library was constructed with the QuantSeq 3ʹ' mRNA-Seq Library Prep Kit. For miRNAseq, NEBNext Multiplex Small RNA Library Prep kit was used according to manufacturers' instructions.