GSM8082939: Whole-exome sequencing analysis of NOOC1 cells; Mus musculus; OTHER
Genomic DNA was extracted from HNM007, AKR and NOOC1 cells by phenol–chloroform extraction and then purified with TIANamp Genomic DNA Kit (Spin Column). DNA concentrations were quantified using Qubit and quality was confirmed by gel electrophoresis. Whole-exome libraries were constructed with the Agilent SureSelectXT Mouse All Exon Kit. Libraries were constructed using 600 ng of DNA and were sequenced on Illumina NovaSeq 6000 platform using 150 bp paired-end reads at the Rhelixa Inc, Tokyo, Japan.