pacbio seqencing data
SMRTbell libraries were constructed according to the standard protocol of PacBio using 15 kb preparation solutions (Pacific Biosciences, CA). The library preparation was mainly divided into the following five steps: 1) genomic DNA shearing; 2) DNA damage repair and A-tailing; 3) ligation with hairpin adapters; 4) nuclease treatment of SMRTbell library; 5) size selection and binding to polymerase.