GSM7712893: RNAseq_NC_2; Rattus rattus; RNA-Seq
Samples were homogenized in Qiazol Lysis Reagent (Qiagen, Germantown, MD) using a TissueLyser II (Qiagen, Germantown, MD). RNA was isolated using a RNeasy Lipid Tissue Mini Kit (Qiagen, Germany). RNA-seq libraries were constructed from total RNA using polyA selection followed by NEBNext UltraDirectional kit workflow (New England Biolabs, MA).