GSM6729801: sample 1_Huh-7 empty cells; Homo sapiens; RNA-Seq
RNA was harvested using Trizol reagent (thermofisher, 15596018). Approximately 1 ug of total RNA was used for the construction of sequencing libraries. Following purification, the mRNA was fragmented  into short fragments using divalent cations under elevated temperature (Magnesium RNA Fragmentation Module  (NEB, cat.e6150, USA) under 94℃ 5-7min). Then the cleaved RNA fragments were reverse-transcribed to create  the cDNA by SuperScript™ II Reverse Transcriptase (Invitrogen, cat. 1896649, USA)