GSM6809659: cGAS KO neutrophils, untreated 1; Mus musculus; RNA-Seq
Total RNA was extracted from neutrophils using  RNeasy mini kit (Qiagen). Approximately 300 ng of the total RNAs from each sample was used to create individually indexed strand-specific RNA-Seq libraries by using QIAseq Stranded mRNA Library kits (QIAGEN). The reactions were subjected to fragmentation and cDNA synthesis. QIAseq Beads (QIAGEN) were used to separate the cDNAs from the reaction mix. Indexing adapters were ligated to the cDNAs, and subsequently all cDNA libraries were inspected for quality by using Agilent 2100 Bioanalyzer (Agilent) and quantified with DeNovix fluorometer (DeNovix). The indexed sequencing libraries were pooled in equimolar quantity and subjected to cluster generation and paired end 150 nucleotide read sequencing on Illumina HiSeq sequencer.