Replicate 1 of phototrophic mixed culture grown in Nitrogen zero testing condition.
Phototrophic Mixed Culture enriched with PNSB were grown in a media containing fuel synthesis water, diluted 5x with the addition of 20 mL/L of trace elements solution and 1 mL/L of vitamins solution in accordance with ATCC medium 2672 and sodium bicarbonate as inorganic carbon source to a final concentration of 7.3 g/L. Potassium dihydrogen phosphate = 99 mg/L was used as Phosphorus source and No nitrogen source was used. Genomic DNA was extracted from bacterial samples using DNeasy PowerWater kit following the manufacturers protocols. Extracted DNA concentration was quantified by NanoDrop 2000/2000c and adjusted to 20 ng of DNA/L of sample and sent to Qatar Biomedical Research Institute for sequencing on a MiSeq system (Illumina, USA).