GSM7923252: RORA_OE_capture_hic_1; Homo sapiens; Hi-C
The chromatin DNA was digested with 100 U HindIII (NEB, #R3104S) and labeled with biotin-14-dCTP (Invitrogen, #19518018). The biotin-labeled DNA fragments were then ligated by 50 U T4 DNA ligase (NEB, # M0202S), de-crosslinked and extracted using QIAamp DNA Mini Kit (Qiagen). We then sheared the purified DNA fragments to 300-600 bp, repaired the ends and added A-tails and SureSelect adaptors. After biotin-streptavidin-mediated pull-down, DNA fragments were amplified by PCR. We conducted capture Hi-C of promoters by the SureSelect XT Library Prep Kit ILM (Agilent Technologies, #G9981A), as per the manufacturer's instructions, with custom-designed biotinylated RNA bait library and custom paired-end blockers. After another PCR amplification, promoter capture Hi-C libraries were purified with AMPure XP beads (Beckman Coulter, #a63880) Promoter capture Hi-C-seq