WGS of Akebia trifoliata: leaf
Based on its morphological characteristics, the A. trifoliata species, in its natural habitat of the Tsinling Mountains (1065519E, 341429N), was confirmed by Professor Ruixian Wang and Yunwu Peng (Ankang University). The plant was then transplanted and preserved in the School of Modern Agriculture and Biological Science and Technology, Ankang University. Approximately, 20g fresh leaves were sampled from a single A. trifoliata plant, and the cpDNA was extracted using a modified high salt method (Shi et al. 2012). Approximately 5-10 g of cpDNA was sheared, followed by adapter ligation and library amplification. Then, the fragmented cpDNAs were sequenced using the Illumina Hiseq 2000 platform. The obtained sequences were assembled using SOAP de novo software and reference-based approaches in parallel. The obtained cp genome regions with ambiguous alignments were manually trimmed and considered as gaps. Gaps were filled using the read over-hangs at margins and the PCR method.