GSM7474280: Prime_Boost1, replicate 1, scRNAseq; Mus musculus; RNA-Seq
In each microwell, a magnetic bead was paired with a cell. Each magnetic bead had a unique cell label and multiple molecular labels (unique molecular identifier or UMI) for capturing RNA. When the RNA is released from the captured cells, it can hybridize to the magnetic beads. The magnetic beads were then collected into a 1.5-ml Eppendorf tube for reverse transcription using GEXSCOPER Single-Cell RNA Library Kit to perform cDNA synthesis on single cells. After cDNA synthesis and PCR amplification, Qubit (Thermo, Waltham, MA, USA) was used for cDNA quantification and Fragment Analyzer was used to determine the cDNA fragment size. Following several steps including fragmentation, adapter ligation, purification, PCR amplification, size selection and QC analysis; libraries were constructed before being sequenced with 150 bp paired end reads on Illumina HiSeq X platform.