GSM6893740: FR_4h3; Botrytis cinerea; RNA-Seq
Mycelium of the parent fludioxonil-sensitive strain (S), laboratory fludioxo-nil-resistant strain (LR), and field fludioxonil-resistant strains were obtained as de-scribed above. All of the isolates were grown at the same time for the same amount of time (three days) under the same conditions (shaking at 23°C and 180 rpm) in PDB. The mycelium were then collected at the same time.  RNA was extracted from samples using the TRIzol method (Invitro Corp., Carlsbad, CA, USA).The RNA concentration and quality were assessed using a nanodrop and gelelectrophoresis. The samples were stored at ?80°C. RNA sequencing was conducted by Novogene using an Illumina? (San Diego, CA, USA) based method to generate 20 million 150-bp paired-end reads per sample. The se-quencing library was prepared with the NEBNext? Ultra?RNA Library Prep Kit for Illumina?, and sequencing was performed on the NovaSeq PE150.