GSM5730143: CB-derived HSPCs - ASXL1 Trunc - RNASeq - Rep3; Homo sapiens; RNA-Seq
RNA was isolated from 100,000 edited CB-derived HSPCs using RNeasy Kits mRNA was purified using oligo(dT) coupled magnetic beads and first strand cDNA synthesis was performed using random hexamers followed by second strand cDNA synthesis, end repair, A-tailing, adapter ligation, size selection and amplification