GSM7474351: H1 cerebral organoids, Control, Ru9; Homo sapiens; ssRNA-seq
Total RNA was extracted on site by LC Sciences using the Qiagen, RNAeasy Mini Kit (#74104) Library preparation was carried out by LC Sciences according to their protocol. Briefly, Following purification, the poly(A) mRNA fractions is fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with a strand-specific library preparation by dUTP method. The average insert size for the paired-end libraries was 300±50 bp.