GSM7083939: SAP30BP_RNAseq_dTAG_2h_R1; Homo sapiens; RNA-Seq
RNA-seq: RNA was harvested using TRIzol reagent (life technologies). 1 ug of total RNA was used for the construction of sequencing libraries.   TT-seq: Six in vitro transcribed spike-in RNAs were added to 100ug  total RNA per sample. This was followed by fragmentation by 1M NAOH and biotinylation of the 4sU-containing RNA with 200 µg/ml HPDP-biotin, and then  purified the nascent RNA with  Dynabeads™ MyOne™ Streptavidin T1 (thermo). The 4sU-labeled RNA was eluted  with 100 mM DTT and then purified and concentrated with the RNeasy MinElute Cleanup Kit (Qiagen). stranded cDNA libraries were generated with VAHTS Universal V8 RNA-seq Library Prep Kit  (Vazyme) according to the manufacturer's instructions.