gorilla gut metagenome
Fecal samples (solid phase) were re-suspended and diluted (50%) in phosphate buffered saline (PBS) and then centrifuged at 2500 g for 20 minutes. The supernatant was then filtered using an 0.45M filter. To enrich for viral particles by reduction of host background, stool supernatant was filtered through a 0.45-m filter (Corning Costar Spin-X centrifuge tube filters), and an aliquot of 315 l of filtrate was pretreated before extraction by incubation with different nucleases: TURBO DNase (Invitrogen, Carlsbad, CA); Baseline-ZERO DNase (Ambion, Foster City, CA); Benzonase (NEB); RNAse A (Promega) for 30 min, at 37 C. Total nucleic acids were extracted using Nuclisens EAsymag (Biomerieux) according manufacturers protocol.  For RNA libraries preparation, Trio RNA Kit (Nugen) was used according to manufacturer instructions.