Gut microbiome of farmed Arctic char
DNA from mid and hind gut were extracted using the QIAamp PowerFecal Pro DNA kit. PCR amplification was conducted with with universal prokaryotic primer pair -D-Bact-0341-b-S-17 (5-CCTACGGGNGGCWGCAG-3) / S-D-Bact-0785-a-A-21 (5-GACTACHVGGGTATCTAATCC-3 and high-fidelity Q5 polymerase (NEB). Libraries were constructed with Nextera XT barcodes and sequenced on  MiSeq with V3 chemistry and 2 x 300 cycles in paired end modality.