GSM8082722: Drosophila melanogaster egg, 30m-1h AEL, Rep2, Batch1; Drosophila melanogaster; RNA-Seq
To prepare RNA and protein samples, fly embryos and unfertilized eggs were dechorionated with bleach and homogenized in lysis buffer [8 M urea, 50 mM ammonium bicarbonate, 1× protease inhibitor cocktail (Calbiochem, San Diego, CA, USA), and RNasin RNase inhibitor (Promega, Madison, WI, USA)] on ice. To isolate the total RNA, TRIzol reagent was immediately added to an aliquot of the fresh lysate. The remaining lysates were frozen for mass spectrometric (MS) analysis. Total RNA isolated from fly embryos or unfertilized eggs using TRIzol was analyzed with an Agilent 2100 Bioanalyzer for quality checks. RNA-seq libraries were generated by Macrogen, Inc. (Seoul, South Korea) using the TruSeq Stranded RNA LT kit.