GSM8083900: siRNA_aCM_2; Homo sapiens; RNA-Seq
For the overexpression of ATRNL1 we packaged the short isoform (ENST00000609571.5) into lentiviral particles via transfection of HEK293T cells according to the manufacturers protocol (Dharmacon lentiviral packaging kit, Horizon Discovery, TLP5913). The overexpression lentiviral vector was obtained from GeneCopoeia (EX-E1118-Lv242). All hESC-aCMs were transduced with lentiviral particles and selected for overexpression with puromycin.  Total RNA was extracted from atrial iPS-CMs using the Direct-zol RNA Miniprep kit (Zymo Research, R2051). RNA-seq libraries were generated with a ribosomal depletion protocol (KAPA RNA HyperPrep with RiboErase, KK8560). All RNA-seq libraries were sequenced on an Illumina NovaSeq. Total RNA-seq, via ribosomal RNA depletion (KAPA Hyperprep RNA-seq kit with RiboErase)