CD19 CAR knock in at TRAC at 3' end in donor 7 Fwd
Genomic DNA samples were isolated using a GeneJET Genomic DNA Purification Kit (ThermoFisher Scientific). TruSeq or Nextera partial adaptor PCR primers were designed targeting both 5-end and 3-end of the integration sites, using one primer specific to the insert sequence and the other specific to the genomic sequences outside of the HMEJ homology arms. PCR was performed using AccuPrimeTaq DNA Polymerase System (ThermoFisher Scientific) at 94C, 2 mins; (94C, 30 sec, 55C 30 sec, 68C 1 min) for 40 cycles, and 68C 5 mins. Expected amplified fragments were purified with a QIAquick Gel Extraction Kit (Qiagen). PCR products were sequenced by Illumina MiSeq Next Generation Sequencer (Genewiz).