amplicon sequencing of mammals:  calf feces
The v3-v4 regions of bacterial 16S rRNA genes were amplified by using primers 341 F 5- CCTACGGGNGGCWGCAG)3 and 806 R 5- GGACTACHVGGGTATCTAAT-3.  Purified amplicons were sequenced using the Illumina NovaSeq 6000 platform to obtain 150-bp paired-end reads.