GSM7011662: THEP Aza Atra_H3K27ac_1; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated. Sequencing libraries were generated and barcoded for multiplexing according to Illumina recommendations with minor modifications. Briefly, 2–8 ng Input or ChIP DNA was end- repaired and A-tailed. Illumina Truseq adaptors were ligated, and libraries were PCR-amplified using KAPA HiFi DNA Polymerase with no more than 12-15 cycles. Libraries were size-selected to exclude polynucleosomes using AMpure Beads. All steps in library preparation were carried out using NEB enzymes (Hasson et al., 2013). Libraries were sequenced on Illumina NextSeq500 (75bp single-end reads).