GSM6893750: nc_input_2; Homo sapiens; ChIP-Seq
For ChIP–seq analysis, briefly, reads from ChIP-seq data for polymerase II antibodies were aligned to the hg19 genome using bowtie2 (v2.3.5.1). Peaks were called using MACS2(v2.2.6) for polymerase II. Different peaks were generated using bdgdiff. The ChIP-seq pileup heatmaps were generated using plotHeatmap. GO/KEGG enrichment analysis was performed using the R package clusterProfiler(v4.0). Visualization was realized by IGV(v2.8.9). Motifs were found to analyze the DNA sequence of poly II protein binding through motifmatchr(v 1.20.0)[1].