RNA-seq of Shoot sample treated with NaCl - Replicate 1
RNA seq-analysis which was performed from the shoot tissues of IR-29 in the absence of allantoin (0.01 mM10 M) after 5 days of salt treatment (100 mM NaCl). Total RNA was extracted from 100mg of tissues by using Trizol reagent (Invitrogen, Carlsbad, USA). Residual genomic DNA contaminations was removed by treating with DNase I (Thermo Fisher Scientific, San Jose, USA). The quality and quantity of the purified RNA was analyzed by gel electrophoresis (2% agarose gel) and Nanodrop (Nanodrop 8000, Thermo Scientific, USA) respectively. The equal quantity of the isolated RNA (three biological replicates) was sent for sequencing by Illumina Genome Analyzer II.