GSM7976953: 1.5% DMS treated lncRNA Panda + ASO2 replicate 3; synthetic construct; OTHER
All RNA was acquired from in-vitro transcription of PCR amplified cDNA or gene-blockes. The libraies for the HIV-1 RRE were prepared using the NEBnext RNA library prep kit. The libraries for the lncRNA PANDA were prepared using the Broad range RNA Xgen kit form IDT. The reverse transcriptase was changed to the Induro RT from NEB for the purposes of our experiment.