Illumina MiSeq of diatoms in mud snail Ilyanassa obsoleta ingesta and feces
DNA extracted using Qiagen DNeasy PowerSoil Kit with some modifications to increase yield; amplicon libraries prepared following a modified version of the the Illumina 16S metagenomic library prep protocol using primers D512for + D978rev (Zimmermann et al. 2011), amplifying a portion of the small subunit ribosomal RNA gene; sequencing performed on an Illumina MiSeq (2 x 300 bp) twice.