RNA-Seq of Mus musculus: adult lateral septum
Total RNA was extracted from flash-frozen lateral septum using Trizol and purified using an RNeasy Micro column (Qiagen). Paired-end, strand-specific sequencing libraries were prepared using TruSeq Stranded Total RNA Ribo-Zero Gold Library Preparation kit (Illumina) and sequenced on an Illumina HiSeq 3000.