RNA-seq of P.berghei activated gametocytes
Total RNA was extracted from activated gametocytes and schizonts of WT-GFP and NEK1clag parasites (two biological replicates each) using RNeasy purification kit (Qiagen). Strand-specific mRNA sequencing was performed of total RNA and using TruSeq stranded mRNA sample prep kit LT (Illumina).