GSM8046465: small RNA-seq: ago18HM-0.5mm-rep1; Zea mays subsp. mays; ncRNA-Seq
Fresh anthers were snap-frozen in liquid nitrogen and total RNA was extracted using the TRI reagent (Sigma-Aldrich) or the TraPR Small RNA Isolation kit (Lexogen; for TraPR sRNA-seq only) sRNA-seq libraries were prepared from ~100 ng total RNA per sample using a Somagenics RealSeq-AC miRNA library kit following the manufacturer's protocol. RNA-seq (Smart-seq2) and nanoPARE libraries were prepared from 5 ng total RNA per sample using the nanoPARE library preparation protocol.