Naganishia albida strain 5307AI, Assembly
Cultures were recovered from glycerol freezer stocks by streaking onto potato dextrose agar (PDA). An isolated colony was re-suspended in tryptic soy broth (TSB) and grown overnight at 27C on a rotary wheel. A 1 mL aliquot of overnight culture was harvested and the pellet was used for DNA extractions. Genomic DNA was extracted using the Qiagen Power Microbiome kit according to the manufacturer's instructions and the DNA was quantified using the Qubit dsDNA assay (Life Technologies). Sequencing libraries were prepared using the Nextera DNA Flex Library preparation kit following the manufacturers instructions (Illumina, San Diego, CA) and sequenced using 150bp paired-end sequencing on an Illumina NextSeq 550.