combined miRNA/mRNA-seq: P62 mouse cortex
Total RNA was isolated using the Direct-zol RNA Miniprep kit (Zymo). QIAseq miRNA library QC and ERCC spike in RNAs were added to 50 ng of RNA per sample. Libraries were generated using the NEXTFLEX Combo-Seq mRNA/miRNA kit (Perkin Elmer) and sequenced on the NovaSeq 6000 (Illumina).