GSM6685222: A7; Homo sapiens; RNA-Seq
Minced skin biopsy specimens were treated with TRIzol solution (Invitrogen) followed by homogenization.  After addition of the TRIzol-tissue homogenate to chloroform, samples were shaken, and spun down. The supernatants were collected, placed on ice, mixed with an equal volume of 70% ethanol, and loaded onto RNeasy Mini Spin Columns, followed by completion of the RNeasy Mini (QIAGEN) protocol. Libraries were prepared using the SMARTer Stranded Total RNA-Seq Kit v2-Pico Input Mammalian kit (Takara Bio USA, Inc.) Libraries were sequenced on an Illumina HiSeq 3000 (1 x 50bp read length). Libraries were diluted to 3nM and pooled for sequencing.