GSM7977780: GFP_MCHERRY_scRNAseq; Mus musculus; RNA-Seq
Hepatocytes and NPCs were isolated using Liberase-based perfusion. After OptiPrep density gradient solution-treated centrifugation, whole liver tissue is fully digested. We further treated, washed and collected the single cell suspension. 10X Genomics Chromium Single Cell 3' Library and Gel Beads Kit (version 3) according to manufacturer's protocol. Libraries were sequenced on an Illumina NextSeq 500 with high output of 400 million reads.