RNA-seq of Euonymus japonicus: adult leaves
To establish a transcriptome dataset for gene differential analysis, we performed transcriptome sequencing, assembly, and annotation for E. japonicus.Trizol reagent was used to extract total RNA from leaves of E. japonicus. The purity of the RNA (OD260/280) was tested using a UV spectrophotometer NanoDrop. The mRNA was enriched by magnetic beads with Oligo(dT), and then a fragmentation buffer was added to break the mRNA into short fragments. The quality of cDNA libraries was tested using an Agilent 2100 Bioassay System (Agilent Technologies, Palo Alto, CA, USA). High-throughput sequencing was performed on three independent replicate samples of the early autumn and winter groups using an Illumina HiSeq4000 sequencer.