GSM7711396: DARID_M_3h_cohort2_2; Mus musculus; RNA-Seq
Total RNA was extracted from hippocampal samples with 1ml TRIzol (Thermo Fisher Scientific) and further purified with the Monarch Total RNA miniprep kit (New England Biolabs) following the manufacturer's recommendations (including DNAse treatment step). mRNA quality was analysed using Agilent Total RNA 6000 Pico on a Bioanalyser (Agilent, 2100). Pair-end sequencing (150bp read length) was performed on the Illumina Novaseq 6000 platform. Library prep was performed by Novogene (Cambridge, United Kingdom): Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.