GSM7474908: Rd10_Female_TMB; Mus musculus; RNA-Seq
Eyes were enucleated for retinal tissue isolation. After removing the anterior chamber and lens, the retina was dissected from the posterior eye cup. Then, the retinal cells were dissociated using the Papain Dissociation System (#LK003153, Worthington Biochemical) following the manufacturer's instructions. In each experimental group, one retina each from n=4 mice were pooled and used for single cell RNA-seq (scRNA-seq). For each group, freshly dissociated cells (~16,500) were loaded into a 10× Genomics Chromium Single Cell system using v3 chemistry following the manufacturer's instructions. Libraries were pooled and sequenced on Illumina NovaSeq6000 platform.