eDNA: SMS2 periphery 350m OMZ
50 ng purified amplicon from each sample was end-repaired (NEBnext ultra II end repair kit, New England Biolabs, MA, USA), cleaned up with 1X Sera-MagTM Select beads (Cytiva,# 29343057). Barcode ligation was performed with NEB blunt/ TA ligase (New England Biolabs, MA, USA) and cleaned with 1x Sera-MagTM Select beads (Cytiva,# 29343057). Qubit quantified barcode ligated DNA samples were cleaned up with 1x Sera-MagTM Select beads (Cytiva,# 29343057). An equimolar concentration of purified barcoded samples was pooled and sequencing adaptor (AMII) was ligated using NEB blunt/ TA ligase (New England Biolabs, MA, USA). Adaptor ligated pooled library was cleaned with 0.4X Sera-MagTM Select beads (Cytiva,# 29343057) and eluted in 15 l of elution buffer. Final library yield was determined using Qubit dsDNA HS assay kit (Cat no. Q32854).