GSM6685302: sc_healthy_1; Homo sapiens; RNA-Seq
The fresh specimens were subjected to collagenase digestion to isolate the cells. For snRNA, fresh specimens were snap frozen by liquid nitrogen, then 10X protocol of isolating nuclei were applied. Then, approximately 20,000 cells/nuclei were used to acquire a single library of 10,000 cells/nuclei by a Chromium controller. Library was performed according to the manufacter's instructions (single cell 3' v3 protocol, 10x Genomics).