GSM7089497: Muscle vdr 7wk rep 1; Mus musculus; RNA-Seq
The frozen tissue samples were ground in liquid nitrogen, added 1 ml of RNAIsoPlus and allowed to thaw. The samples were extracted for 10 minutes at room temperature followed by addition of 200μl chloroform. After vortexing, the samples were centrifuged at maximum speed for 5 minutes. The aqueous layer was transferred to fresh tubes and precipitated with isopropanol. RNA libraries for RNA-seq were prepared using TruSeq RNAseq library prep kit v2 following manufacturer's protocols.