GSM8003136: SW480_JH_Rep2; Homo sapiens; RNA-Seq
RNA was extracted from using Trizol reagent following standard manufacturer's instructions. RNA integrity and quantification was  assessed using the Agilent TapeStation instrument and High Senstivity RNA ScreenTape and Qubit RNA assay kit all RIN score were higher than 9. Poly A enrichment from total RNA was performed, and library were prepared using Agilent Technologies: Poly A enrichment from total RNA, cDNA Synthesis (Cat #5500-0135), cDNA library prep, (cat# 5500-0140 and cat# 5190-6444). Final library were run on Illumina Nextseq 550 sequencer USING Illumina NextSeq 500/550 High Output Kit, as 75-bp single-end reads