Sample info
400 ng of DNA was fragmented and subjected to size selection. DNA end repair and adapter ligation were conducted with MGIEasy PF Adapters-96 Kit. Finally, a total of 75fmol DNA Nano ball library was prepared by combining 50 samples. For long reads, library was prepared using the SQK-LSK109 ligation sequencing kit and sequenced on Nanopore MinION (Oxford Nanopore Technologies, Oxford, UK)