GSM7475317: Region capture Hi-C, CASTxB6, rep2; Mus musculus; Hi-C
Hi-C libraries were generated using the Arima Hi-C kit using 3 ug of chromatin from ESCs or day 6 iNs. End repair and adapter ligation was performed using the Accel-NGS 2S DNA Library Kit (Swift Biosciences). Agilent SureSelect probes against the Peg13-Kcnk9 locus were designed against mm10 chr15:71850001-73350000. Probes overlapping musculus/castaneous SNPs were designed to perfectly match both genotypes (i.e. two probes per SNP). End repaired and adapter ligated Hi-C libraries were then enriched using Agilent SureSelectXT HS Target Enrichment System.