GLOSmajoP1P
RNA quantitation and quality checks were conducted on an Agilent 2100 Electrophoresis Bioanalyzer (Agilent, USA) utilizing an RNA 6000 Nano chip. Poly(A) mRNA isolation and cDNA library construction was performed using NEBNext Poly(A) mRNA Magnetic Isolation Module and Ultra RNA Library Prep Kit for Illumina (NEB, Australia), respectively as per the manufacturers instructions. Paired-end (2 x 250 bp) sequencing was performed on an Illumina NextSeq 500 platform (Illumina Technologies, USA) at the Biomolecular Resource Facility (BRF), The Australian National University.