GSM6620522: K562 cells, SRSF2P95H, rep1; Homo sapiens; RNA-Seq
Total RNA was purified with the RNeasy kit (Qiagen) and treated with the RNase-Free DNase Set (Qiagen) to remove the residual DNA. Total RNA samples from HeLa cells was then subjected to rRNA depletion using Ribominus Human/Mouse Transcriptome Isolation Kit (Invitrogen). Enrichment of mRNA from total RNA sample in K562 cells was performed using GenElute™ mRNA Miniprep Kit (Sigma-Aldrich). RNA libraries for RNA-seq were prepared using KAPA Stranded mRNA-Seq kit according to the manufacturer's instructions.