GSM7473543: RA_patient_4; Homo sapiens; RNA-Seq
Cartilage were lysesd and pelleted by centrifugation at 300 g for 3 minutes at 4 °C and washed with cold PBS. Tissues were resuspended in 1 mL pre-warmed (37 °C) TrypLE, and rotated at 37 °C for 15 to 30 minutes until tissues were dissociated into single cells via microscope). Cells were pelleted at 300 g for 3 minutes at 4 °C, and washed with cold PBS. Chromium Single Cell 3' Reagents Kits version 2