GSM7892774: murine primary VSMCs, siScarmbled-2, 48hrs; Mus musculus; RNA-Seq
Total RNA was extracted according to the instruction manual of the TRlzol Reagent (Lifetechnologies, California, USA).1 ug of total RNA was used for the construction of sequencing libraries. Sequencing libraries were generated using Hieff NGS Ultima Dual-mode mRNA Library Prep Kit for Illumina (Yeasen Biotechnology (Shanghai) Co., Ltd.) following manufacturer's recommendations