GSM7384125: BIN67, Triptolide, 6h, rep2; Homo sapiens; RNA-Seq
RNA was extracted using Quick-DNA/RNA Miniprep Plus Kit (Zymo Research Cat#D7003), per manufacturer's instructions, with on-column DNase digestion. 500ng of total RNA was used to generate mRNA libraries using KAPA mRNA HyperPrep Kit library prep kit (Roche, cat#08098123702) per manufacturer's instructions. Final libraries were evaluated by TapeStation, quantitated using the Life Technologies/Invitrogen Qubit, and equimolarly pooled. Paired-end sequencing was performed on a NovaSeq6000 using a NovaSeq S4 300 cycle kit (cat#20028312) (151x11x11x151).