miRNA-Seq of human fetal neocortex
Total RNA was extracted using miRNeasy-mini (QIAGEN 217004) kits or was extracted using trizol with glycogen followed by column purification. Library preparation for small-RNA was conducted using TruSeq Small RNA Library Prep Kits (Illumina RS-200). RNA libraries were randomized into eight pools and run across eight lanes of an Illumina HiSeq2500 sequencer at 50 base-pair, single-end reads.