GSM6932380: Fasted; Mus musculus; OTHER
Livers were embedded in tissue-Tek (OCT) and snap-frozen using dry ice slurry. Cryosections were cut at 10-μm thickness, mounted onto the Visium Spatial Gene Expression Slide (10x Genomics, PN-2000233). Sections were placed on Thermocycler Adaptor with the active surface facing up, incubated for 1 min at 37 °C, and fixed for 30 min with methyl alcohol in –20 °C, followed by staining with H&E (Eosin, Dako CS701, Hematoxylin Dako S3309, bluing buffer CS702). The brightfield images were taken on VS120 (Olympus) whole-slide scanner. Visium spatial gene expression was processed using Visium spatial gene expression slide and Reagent Kit (10× Genomics, PN-1000184). For each well, Slide Cassette was used to create leakproof wells for adding reagents. 70 μL Permeabilization enzyme was added and incubated at 37 °C for 20 min. Each well was washed with 100 μL SSC, and 75 