GSM6932338: 5B-female-Bmal1-ZT0-control_R1; Mus musculus; ChIP-Seq
Samples were processed using the SimpleChIP Plus Sonication Chromatin IP Kit #56383 (Cell Signaling Technology, Inc.) according to manufacturer's instructions. Briefly, finely chopped samples were cross-linked with 37% formaldehyde for 12.5 min at room temperature. Isolated chromatin was fragmented using the Diagenode Bioruptor (ON 30 sec, OFF 45 sec) for 35 mins. An equal amount of chromatin from each sample was immunoprecipitated with normal rabbit IgG (#2729P) or Bmal1(D2L7G) rabbit mAb (#14020S), both antibodies were purchased from Cell Signaling Technology.  Libraries were prepared using the ThruPLEX DNA-Seq Kit (Takara Bio USA). Indexing was performed using the SMARTer DNA HT Dual Index Kit (Takara Bio USA).