DNA metabarcoding of wheat endophytic microbiome: bacterial profiling with gITS7ngs/ITS4ngsUni primer sets with PNA_ITS2 (rep 1)
Endophytic microbial DNA was extracted from wheat leaf tissues. PCR amplifications were then carried out to amplify a bacterial or fungal marker sequence in a metabarcoding approach. For some samples, a developed PCR clamp was added in the mix, with the aim of blocking host (wheat) DNA co-amplification. Amplicons were sequenced on a 2x300 bp MiSeq Illumina device.