GSM7049405: A_8; Homo sapiens; RNA-Seq
Adherent cells were detached with Accutase. mRNA was extracted using the Qiagen RNeasy Plus Kit. mRNA libraries were prepared using the KAPA mRNA HyperPrep and Illumina TruSeq adapters. Libraries were quantified using a Qubit Fluorometer validated using the Agilent High Sensitivity D1000 Tapestation Kit. Libraries were pooled 12/sequencing run and sequenced on 75 cycle High Output v2.5 Illumina kits.