GSM6591366: hPES-blastoids; Homo sapiens; RNA-Seq
hPES-blastoids and hBES-blastoids were manually picked up using mouthpipette and washed three times. ~150 hPES-blastoids or hBES-blastoids were harvested and dissciated with a homemade enzyme mix composed of 0.5 X versene (Lonza, 17711E), 0.5 X Acumax (Innovative Cell Tech, AM105), and 0.05 X Dnase (STEMCELL Tech, 07900) at 37°C for 30 min with agitation. Dissociated cells were spinned down and wash with PBS + 0.05% BSA for three times and resuspended in the same buffer. Cell density was determined by a TC10 cell counter (Biorad, 1450001). Dissociated cells (~6000 cells for hPES-blastoids and ~8000 cells for hBES-blastoids) were loaded into the Chromium single cell controller (10x Genomics) to generate single-cell gel beads in the emulsion according to the manufacturer's protocol. The library was generated using the Chromium Single Cell 3' Reagent Kits according to the manufacturer's manual. The two libraries were pooled and sequenced using Nextseq 500 (150 cycles, high output).