GSM6021957: dialout MOLM13_RNAseq_single_5; Homo sapiens; OTHER
We used 6000 cells per channel, for 15 channels for the "single" experiment, and 3 channels for the "combo" experiment. The “single” and “combo” experiments were profiled with 15 and 3 channels, respectively, and were sequenced paired-end on an Illumina Hi-Seq instrument at a ratio of 3 channels of 10x to 1 lane of sequencing (5 for the “single” experiment, and 1 for the “combo” experiment), using paired end reads as follows.     For the “single” experiment, read 1 is 28 bases long (16 for cell barcode, 12 for UMI), read 2 is 91 bases long and sample index read is 8 bases long.     For the “combo” experiment, read 1 is 28 bases long (16 for cell barcode, 12 for UMI), read 2 is 91 bases long and sample index read is 8 bases long.     Perturbations were assigned to cells using dial-out PCR. Dial-out PCR products were sequenced on an Illumina Mi-seq, paired-end (for all experiments, read 1 is 28 bases and read 2 is 60 bases long), with read 1 having the same structure as in the high-throughput sequencing of the libraries described above. Each experiment required only one Mi-Seq run to process the dial-out libraries.