GSM8046399: cph1-delta,H4Ac, ChIP,rep2; Schizosaccharomyces pombe; ChIP-Seq
Cells were fixed with 1% formaldehyde for 15min. After quenching and cell lysis, chromatin was sonicated using Sonicator with duty cycle 90% (0.9 sec ON; 0.1 sec OFF) 12 pulses for 5 times. The ChIP-seq library was constructed using VAHTS® Universal DNA Library Prep Kit for Illumina V3 (Vazyme, ND607). 2 ng of DNA was used as starting material for input and ip samples. Libraries were amplified using 14 cycles on the thermocycler. Post amplification libraries were size selected at 250-500 bp in length using Qiagen gel purification kit.