Rep-Seq of homo sapiens: peripheral IgM B cells from healthy control donor spiked with 10000 MEC-1 cell line cells
RNA was isolated from 100,000 cells and 5'RACE was performed by template-switching. cDNA was amplified with a universal forward primer and IgG heavy and light chain specific reverse primers in a nested PCR. Libraries were prepared with the TruSeq Nano DNA Library Prep Kit. Sequencing was performed with the MiSeq Reagent Kit v3, 2x300 bp.