ITS-AMPLICON_Fungi
Microbial communities were assessed by sequencing bacterial 16S rRNA, fungal ITS, and protist 18S rRNA genes using the primer sets 515F (Parada et al. 2016) and 806R (Apprill et al. 2015), ITS1F and ITS2 (White et al. 1990), and 1391F (STOECK et al. 2010) and EukBr (Amaral-Zettler et al. 2009), respectively. Sequencing was performed at the University of Minnesota Genomics Center (Minneapolis, MN) utilizing the Illumina MiSeq V3 300 bp pair-end technology. Libraries were prepared using a modified protocol as described by Gohl et al. (Gohl et al. 2016).