RNASeq of Enchytraeus albidus PL-A strain
Specimens in live form on agarose gel were subjected to isolation using the Total RNA Mini kit (A&A Biotechnology) with manual mechanical homogenization. The isolation was carried out on 4 specimens (one pooled sample). 50 uL of the eluate was subjected to genomic DNA removal reaction using the Clean Up RNA Concentrator kit. Library was prepared for sequencing using TruSeq Stranded mRNA LT Sample Prep Kit according to TruSeq Stranded mRNA Sample Preparation Guide, Part # 15031047 Rev. E