16S retal swabs: Problematic cannabis use is associated with reduced rectal microbial species richness and diversity among a pilot sample of young men who have sex with men
Sequencing of the V4 region of the bacterial 16S rRNA gene was performed using the 515FB-806RB primer set, Earth microbiome project Protocol was used for processing of samples.  Sequenced with a 2x300 bp cycle with 20% PhiX.