GSM6320728: EndoC-βH3 cells, Senescent cells, H3K27me3 4; Homo sapiens; OTHER
Genome wide chromatin profiles were generated using 500,000 cells per sample and MINT (Multiplexed indexed T7 ChIP-seq) v2 protocol. Cells were lysed and digested with MNase separately to yield fragments of 1-5 nucleosomes. DNA fragment ends were ligated to adaptors having sample barcodes (#index 1). Barcoded DNA fragments from each sample were pooled and splited for separate ChIP reactions with antibodies.  After antibody pull down,  the purified DNA was linearly amplified and barcoded (#index 2, antibody brcode) to get the MINT-ChIP library. The library was sequenced by using Illumina NextSeq 500 instrument as paired-end.