GSM7658352: Adult, snRNAseq; Mus musculus; RNA-Seq
Preparation of nuclear suspensions from frozen dermal tissue samples required 200mg or "soybean" sized tissue samples. After tissue collection, non-adherent tissue was removed, residual blood and other fluids were properly drained, and the tissue was cut into 1-2mm3 pieces on ice in a centrifuge. The fragments were transferred to cryotubes and flash-frozen in liquid nitrogen for 5 minutes, after which they could be transferred to an ultra-low temperature refrigerator or stored in liquid nitrogen. The entire sampling and minification process should be completed quickly to avoid RNA degradation. Libraries were generated and sequenced from the cDNAs with Chromium Next GEM Single Cell 3' Reagent Kits v 2. cDNA libraries were sequenced on an Illumina Novaseq6000 platform (Illumina).