GSM7119080: h3k18la_nf_rep1; Gallus gallus; OTHER
Neural crest cells were obtained by micro-dissecting dorsal neural folds (NFs) from HH9 embryos (n=5 embryos, n=10 NFs) and pre-somitic mesoderm (PSM) tissue was obtained by dissecting the posterior region of HH9 embryos (n=5 embryos, n=10 PSM strips). A cell suspension was achieved by dissociating embryonic tissue with Accumax (Innovative Cell Technologies, AM105) for 30 min at room temperature under mild agitation and gentle pipetting halfway through the incubation time. The cells were then subjected to the low-salt CUT&RUN protocol (as described in dx.doi.org/10.17504/protocols.io.zcpf2vn and the Methods section of the present study). DNA libraries for CUT&RUN experiments were performed using the NEBNext Ultra II DNA Library Prep Kit (NEB, E7645) following the manufacturer's protocol. Quality control of prepared libraries was performed using an Agilent 4200 Tapestation with D5000 Reagents (Agilent Part# 5067-5589) and D500 ScreenTape (Agilent Part# 5067-5588). Libraires were pooled to equimolar concentrations using the NEB Library Quantification Kit (NEB E730S) and sequenced with paired-end 37-bp reads on an Illumina NextSeq 500 instrument.