Construct MT95; transformation event 1; chloroplast-expressed single promotor producing hairpin/loop doubled-stranded RNA targeting the trips SNF7 nuclear gene; tobacco whole-cell RNA; sRNA raw reads; biological replicate 3
Whole-cell total RNA was isolated from whole seedling at early first leave stage excluding roots; sRNA libraries were constructed using the RealSeq-AC from Somagenics using 150 ng of total RNA and amplified with 16 PCR cycles. Sequencing was done with the Illumina NextSeq 550.