GSM6685260: iNeuron_WT_day30_RNAseq_rep2; Homo sapiens; RNA-Seq
To extract RNA, cells were plated on 6-well dishes, washed with PBS, and then 500 L of tri-reagent (Zymo research corporation, Cat. No. R2050-1-200) was added directly to the cells. The lysed cells were collected using a cell scraper. To isolate RNA, we used a Direct-zol RNA miniprep kit (Zymo Research Corporation, Cat. No. R2052), following manufacturer's instructions including the optional DNAse step.For RNA sequencing, two biological replicates were sequenced. Total RNA was enriched for polyA and sequenced 2x75 bp on a NextSeq550 machine.