single-cell and single-nucleus RNA-Seq of homo sapiens: human tumor
Single cells or single nuclei were loaded into channels of a Chromium single-cell 3' Chip (10x technology) and processed using either the V2 10x or V3 10x technology. Single cells or single nuclei were partitioned into droplets with gel beads in the Chromium Controller following the manufacturer's protocols. This was followed by polyA capture of RNA, barcoded reverse transcription, cDNA amplification, fragmentation and adapter and sample index attachment.