GSM5603464: HEK293_1; Homo sapiens; RNA-Seq
Total RNA was extracted using TRI reagent (Sigma) according to the manufacturer's instructions. DNase treatment was performed using TURBO Dnase at 37°C for 15 min and RNA was re-purified using the RNA clean and concentrator kit (Zymo Research) according to the manufacturer's instructions. Ribosomal RNA depletion was performed and libraries were constructed by TruSeq total RNA library preparation (Illumina) according to the maufacturer's instructions.