Straw degrading simplified microbiome fermentation solution replicate 3
The precipitate samples from the sixth day of Y and YL treatments were used for the microbial community analysis. The genomic DNA was extracted using the E.Z.N.A. soil DNA kit (Omega Bio-tek, Norcross, GA, USA) according to the instructions. The primers (515FmodF 5'-GTGYCAGCMGCCGCGGTAA-3', 806 RmodR 5'-GGACTACNVGGGTWTCTAAT-3') were used to PCR amplified the V4 region of bacterial 16S rDNA genes. PCR products were purified and then sequenced using the MiSeq PE300 platform (Illumina, USA) at Majorbio Bio-Pharm Technology Co. Ltd. (Shanghai, China). Following this, DNA samples were sent to Majorbio Bio-Pharm Technology Co. Ltd. (Shanghai,China) for 16S and internal transcribed spacer (ITS) rRNA gene sequencing on a MiSeq PE300 platform (Illumina, USA). FASTP (v. 0.19.6) and FLASH (v. 1.2.11) were used to quality-filter and assemble the raw sequences. Raw sequences were processed on the QIIME (v. 1.9.1) platform to generate abundance tables for each taxonomic level .After completing data quality control and splicing, UPARSE (v. 11) was used to cluster and match operational taxonomic units (OTUs) with > 97% similarity, and to identify and remove chimeric sequences. Ribosomal Database Project (RDP) Classifier (v. 2.13) was used to assess the taxonomy of 16S rDNA sequences against the Silva v.138 databases