RNA-Seq of organic cows receiving a pasture and TMR or TMR only diet
The sequencing library was prepared using an library preparation process in which PCR reactions were performed in real-time PCR machines to control cycles and therefore limit PCR chimera formation. The final PCR products were quantified with qPCR fluorescence readings and pooled together based on equal molarity. The final pooled library was cleaned up with the Select-a-Size DNA Clean & Concentrator (Zymo Research, Irvine, CA), then quantified with TapeStation Santa Clara, CA) (Agilent Technologies, (Thermo Fisher Scientific, Waltham, WA).