GSM7658351: MC38T_2,scRNAseq; Mus musculus; RNA-Seq
Control or Atp6v0a1 knockdown MC38 tumors were dissected from C57BL/6J mice. The dissections were done in ice-cold Ca2+-free HBSS solution and the tumor tissues were dissociated to single cells using the mouse tumor dissociation kit (Miltenyi Biotec) as the user manual. Following the lysis of red blood cells with easysep RBC lysis beffer (STEMCELL), the cells were washed and resuspended in HBSS buffer. After cell counting and  cell-viability measurement, the GEM Generation & Barcoding were performed using Chromium Next GEM Single Cell 3' Reagent Kits v3.1. Libraries were prepared according to Chromium Next GEM Single Cell 3' Reagent Kits v3.1. Briefly, silane magnetic beads were used to purify the first-strand cDNA from the post GEM-RT reaction mixture. The full-length cDNA were amplified via PCR to generate sufficient mass for library construction. After end repair, A-tailing,adaptor ligation and PCR amplification, the final libraries were ready for sequencing.