GSM7977776: Heart, young, hashtag oligos; Mus musculus; RNA-Seq
Myocardial infarction as induced in C57Bl6/J female mice by permanent occlusion of the left anterior descending artery ligation. At post-MI Day 5, cells were isolated from the infarct zone of young (3-5 months) and old (15-17 months old) and run for scRNA sequencing. Droplet-based snRNA-seq libraries were prepared using the Chromium Single Cell 3' v3.1 according to the manufacturer's protocol. Libraries and sequencing were performed at the Translational Sequencing Core at MUSC. Antibody-derived tag (ADT) libraries were constructed using TotalSeq-A anti-mouse for CD8a, CD44, CD62L, NK1.1, TCRgd, and CD3. Three biological replicates were used for each category (young and aged); these replicates were each tagged with TotalSeq B anti-mouse hashtags 2, 3, and 4 and multiplexed.