GSM5751218: D09-Control-Bhlhe40-WT; Mus musculus; RNA-Seq
Established tumors were excised from mice at day 9 (D09) or Day 11 (D11) post-transplant, minced and dissociated using 1 mg/ml type IA collagenase (Sigma). Introtumoral immune cells were stained with a monoclonal anti-mouse CD45 antibody and Zombie NIR Viability dye and sorted on a BD FACSAria II fluorescence-activated cell sorter. Approximately 10,000 cells per sample were processed using Chromium Next GEM Single-cell 5' Reagent Kits v2 (10x Genomics) Libraries were prepared using Chromium Next GEM Single-cell 5' Reagent Kits v2 according to manufacturer's protocol (10x Genomics)