RNA_Seq of Batrachium bungei: young fresh leaf
Total RNA was separately extracted from young leaves of each individual using the QIAGEN RNeasy Plant Mini Kit (QIAGEN, Germany). RNA samples were then treated with DNaseI and purified using Dynabeads  Oligo (dT)25 (Life Technologies, Carlsbad, CA, USA) and 150-bp PE libraries were constructed following the manufacturers instructions for the NEBNext  Ultra TM RNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA). The generated cDNA libraries were amplified using the TruSeq PE Cluster kit (Illumina, San Diego, CA, USA) and sequenced on the HiSeq4002 platform at Majorbio Bio-Tech (Shanghai, China).