GSM6022308: D8.B6.B6.FO.3; Mus musculus; RNA-Seq
Spleens were harvested at time of saccrifice and lypmhocytes were stained and double FACS sorted directly into TRIZOL reagent as follows:  follicular B cell: live, singlet, CD19+, CD93-, CD23+, CD21/35int.  marginal zone B cell: live, singlet, CD19+, CD93-, CD23-, CD21/35high. intermediate B cell: live, singlet, CD19+, CD93-, CD23int/+, CD21/35int/+ RNA was extracted by TRIZOL protocol and quantified by Qubit.  cDNA was prepared using Takara Clonetech SMART-Seq HT kit by protocol with Illumina Nextera XT reagents for library construction and quantified and equal molar pooled by tape station and qubit analysis.