Single Cell data of B cells from patients with chronic Hepatitis C and sustained viral response
Single Cell data of B cells from patients with chronic Hepatitis C and sustained viral response
Peripheral blood of HCV patients was collected in CPT tubes. PBMC were isolated by centrifugation and cryopreserved in liquid nitrogen. To enrich B cells for single-cell analyses, we FACS-sorted CD19+ cells. Cells were processed on a 10X Chromium Controller. Single-cell libraries were generated using the Next GEM Single Cell 5' Kit v2 and Chromium Single Cell Human BCR Amplification kits.