Cp1_ReplicateB
Cultures were grown in YPD at 30degC at a starting optical density (at 600nm) of 0.1, inoculated from a saturated 24 hr culture, and grown to mid-log phase. Cells were then collected by centrifugation, and total RNA was extracted using the RiboPure Yeast kit (Invitrogen) with an additional isopropanol precipitation after DNase treatment.