16S rRNA amplicon Nanopore of Rhopalosiphum padi Endosymbionts
Bacterial universal primers FD1 (AGAGTTTGATCCTGGCTCAG) and RP2 (ACGGCTACCTTGTTACGACTT), amplify total endosymbiont community. Amplicon libraries generated using Native Barcoding Kit 24 V14 (Oxford Nanopores) and sequenced on PromethION 24 instrument using a PRO-114M flowcell