GSM8047372: WT, snRNAseq; Mus musculus; RNA-Seq
Frozen livers from 4 mice of liver-specific LBP KO group or 4 mice of WT group were cut and pooled according to the group. Samples were minced and transferred to the Dounce homogenizer. We added 4 ml of lysis buffer consisting of 10 mM Tris-HCl, 10 mM NaCl, 1 mM MgCl2, 0.1% IGEPAL and 0.3 U/μL RNase inhibitor to pooled liver samples, then homogenized the tissue carefully. WRB buffer (PBS, 2% BSA, and 0.2 U/μL RNase inhibitor) was added and centrifuged to remove the lipid layer. After filter again with 40 μm Flowmi cell strainer, nuclei were pelleted by centrifuging at 500 g for 5 min at 4 °C and resuspended in WRB buffer. Single-nucleus RNA sequencing was prepared using 10X Genomics' Chromium Next GEM Single Cell 3' Kit v3.1 following manufacturer's protocol.