GSM7011362: glp-1, L4440, day 1, rep2; Caenorhabditis elegans; RNA-Seq
Worms were frozen in Tri Reagent, and RNA was isolated with chloroform and isopropanol. RNA was treated with Dnase (TURBO Dnase kit, Invitrogen, AM1907), and cleaned with ethanol precipitation before library preparation. 315ng RNA from each sample was used for QuantSeq 3' mRNA-Seq Library Prep Kit (Lexogen) following the manufacturer's instructions and using 13 PCR cycles. 3' mRNA-seq