18S metabarcoding: soil
PCRs with HotStartTaq PCR Master Mix (Qiagen, Germany), 0.2 mM of each dNTP, 0.5 pmol of each primer and 20 ng of genomic DNA. Thermal cycles: 15 m at 95C; 25 cycles of 1 min at 95C, 1 m at 58C and 1 min at 72C; 7 min at 72C. Amplicons cleaned up with Agencourt AMPure XP (Beckman, USA) and size checked with a Bioanalyzer 2100 (Agilent Technologies, USA). Libraries were prepared following 16S Metagenomic Sequencing Library Preparation protocol.