GSM7839624: WT 3; Mus musculus; RNA-Seq
Mouse lung endothelial cells from WT, DNMAML, Notch1f/f (n=3 for each condition) were sorted into RNA lysis buffer (Qiagen) and stored at -80ºC until RNA extraction. Total RNA was extracted using RNeasy Plus Kit (Qiagen) with inclusion of DNAse treatment step. RNA sequencing libraries for Illumina sequencing were prepared in one batch using strand-specific using CORALL Total RNA-seq Library Prep Kit with Unique Dual Indices with RiboCop HMRv2 rRNA Depletion (Lexogen).