Bees cuticular 16s metagenome A. m. mellifera3
Microbial community DNA was extracted from bees cuticular samples using the Quick-DNA Fecal/Soil Microbe Miniprep Kit according to manufactureres' instructions (Zymo Research, Freiburg, Germany). 16s Amplicons of bees cuticular DNA samples  were generated with the primer set BAC341f (5-CCTACGGGNGGCWGCAG-3) and BAC758R (3-GACTACHVGGGTATCTAAKCC-5) as suggested by Klindworth et al. (2013).