RNA-Seq of Lithobates pipiens: pooled adult olfactory epithelium
Total RNA was extracted from the excised frog epithelium tissue. Both the quality and quantity of this RNA sample met the requirements for Illumina sequencing. An RNA-Seq library was prepared using a TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA) and quantitated by qPCR. The library was sequenced in a 161-cycle, paired-end run using a HiSeq SBS Kit V4 on a HiSeq 2500 platform. Sequencing reads were demultiplexed, adaptor-trimmed and converted into FASTQ format using Illuminas bcl2fastq Conversion software (version 2.17.1.14).The raw reads were of high quality except for bases near the ends. Trimmomatic version 0.33 was used to trim and filter any residual adaptor content and low quality bases with the following settings: ILLUMINACLIP:TruSeq3-PE-2.fa:2:15:10 LEADING:28 TRAILING:28 SLIDINGWINDOW:3:15 MINLEN:30.