Droplet multiple displacement amplification of HG002 DNA
Genomic DNA was mixed with dMDA reagents and encapsulated into single emulsion droplets by Xdrop. In total, 32 reactions were performed. Following a 16-hour incubation, the amplified DNA was isolated subjected to T7 endonuclease I treatment to remove branching. Lastly, DNA repair was performed and Tapestation was used to estimate DNA molecule size (Madsen et al. 2020).