Metagenome of Spent fuel nuclear storage ponds - Indoor system
Pond water samples, collected from a depth of approximately 1 m were transferred to the NNL Central Laboratories (National Nuclear Laboratory, Cumbria, UK). Radioactivity levels were assessed in accordance with the Environmental Permits and Nuclear Site licenses held by Sellafield Ltd. DNA extraction was performed using the PowerWater DNA Isolation Kit (Mobio Laboratories Inc, Carlsbad California, USA). The extracted DNA samples, screened for radionuclide contamination, were subsequently transferred to the University of Manchester and stored at -20C. The complete sequencing process, encompassing standard library preparation, genomic DNA fragmentation, hybridization, and sequencing was executed at Celemics (Celemics, Inc., Seoul, Korea).