GSM7089517: KARR-seq A549 VSV G1 Rep 1; Homo sapiens; Vesicular stomatitis virus; OTHER
Resuspend cell pellets in 410 µL 25 mM K3BO3, with 50 µL 10% SDS, 30 µL proteinase K, 10 µL RNase inhibitor. The mixture was shaken at 55 ˚C for 2 h and was subjected to phenol-chloroform extraction and ethanol precipitation. Extracted RNA were used for enrichment, end repair, and proximity ligation. Libraries were conctructed by using the SMARTer Stranded Total RNA-seq kit v2 – pico input mammalian.