RNA-seq of mus musculus: 2-week-old Pdx1-Cre;LSL-KrasG12D/+ male pancreas
Total RNA was subjected to cDNA synthesis and NGS library construction using the Universal Plus mRNA Seq (Tecan Genomics, San Carlos, CA). The quality and average length of sequence library for each sample was assessed using Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) and either the DNA 1000 kit. The indexed samples were pooled equimolarly and sequenced on Illumina NovaSeq 6000 (150 base, paired end reads) (Illumina, San Diego, CA, USA).