NGS of JCVI-Syn3B
Zymobiomics DNA Miniprep kit was used to extract genomic DNA from JCVI-syn3B cell culture. 40ng DNA was input into the Illumina DNA Prep, (M) Tagmentation (96 Samples, IPB, Lot#20060059) library prep kit. It is a  tagmentation based protocol that resulted in a 675bp length library. The library was loaded at a concentration of 12 pMolarity onto a Miseq v3 flowcell. The sequencing run resulted in a 265Mbase yield with Quality Q30 score of 96.83.