WGS - wild-type C. necator H16
The final strain from the adapted laboratory evolution, ht030b, was streaked on an LB plate containing 3% (w/v) NaCl. Two colonies were grown overnight in liquid LB containing 3% (w/v) NaCl, and the genome was purified from each sample using a Monarch Genomic Purification Kit (New England Biolabs). A single colony of wild-type H16 was grown, and the genome was likewise purified as a control. Extracted genomic DNA was provided to the Vincent J. Coates Genomics Sequencing Lab at the University of California, Berkeley (QB3 Genomics, UC Berkeley, Berkeley, CA, RRID:SCR_022170), which prepared 150 bp paired-end Illumina sequencing libraries. These libraries were then sequenced using a NovaSeq 6000 S4  .