PCR amplification andillumina MiSeq sequencing of vmp expression site in Borrelia miyamotoi following in vitro antibody killing and reisolation.
DNA from B. miyamotoi strain LB-2001 was used as PCR template. PCR amplicons (app. 350bp) were generated of the Vlp25 loci and purified using ThermoFisher KingFisher FLEX technology. The purified amplicons were index using illumina Nextera XT protocol and purified using AMPure xp magnetic beads. The finished library was sequenced using the illumina MiSeq system, and sequences were analyzed using CLC bio software (Qiagen).