GSM7384541: PC3 MTID V7 Y22S 1; Homo sapiens; RNA-Seq
Total RNA was extracted using the SV Total RNA Isolation System (Promega) according to the manufacturer's protocol. Total RNA-seq libraries were then prepared using 800ng of resulting RNA from each sample and NEBNext Ultra II RNA Library Prep Kit for Illumina with Poly(A) mRNA Magnetic Isolation Module (NEB #E7760, E7490 and E6440) according to the manufacturer's protocol with 11 amplification cycles. Libraries were sequenced on a NextSeq550 with paired-end reads of 75 base pairs, with a read depth of 60 million fragments / library. Two libraries from two corresponding biological replicates were prepared for each sample.