GSM8083943: MAP8.B6.Ly49m_KO_nanopore; Mus musculus; OTHER
High molecular weight DNA was extracted from ~5 million splenocytes of 1 MAP8.B6.Ly49h or MAP8.B6.Ly49m KO mouse, using Gentra Puregene Cell Kit (8 x 10^8) (catalog # 158767). 8 ug of DNA was then fragmented using Covaris g-TUBE (catalog 520079), which was spun at 4200 rpm to fragment the DNA to an average size of 50 Kb. The 7.7 ug DNA recovered after fragmentation was subsequently cleaned using Short Fragment Eliminator (Oxford Nanopore EXP-SFE001). The sequencing library was prepared using Ligation Sequencing Kit V14 (ONT SQK-LSK114) with 2 ug DNA as input, and 22 fmol of the library was run on a single PromethION flow cell (FLO-PRO114M) for 42 hours without reloading.