GSM6774197: MI_E_vertical_cross_section, spatial transcriptomics; Mus musculus; RNA-Seq
OCT-embedded freshly frozen hearts of MI and MI-E were used for Visium Spatial Gene Expression platform (10X Genomics). The 10-µm vertical and horizontal sections from MI and MI-E hearts were placed on the pre-chilled Visium Spatial Tissue Optimization slides, followed by optimization of tissue permeabilization according to the manufacturer's protocol, which established an optimal tissue permeabilization time of 18 minutes for four heart sections. After hematoxylin and eosin (H&E) staining, permeabilization, reverse transcription and second-strand cDNA synthesis, the Visium Spatial Single Cell 3′ Gene Expression libraries were constructed, sequencing of which were performed on Illumina NovaSeq 6000. The barcoded libraries were prepared under Visium Spatial Gene Expression Slide & Reagent Kit (10X Genomics) according to the manufacturer's protocol.