Full length cDNA sequencing from tuber tissue of Solanum tuberosum clone DM1S1
RNA from tuber tissue was isolated using a modified hot borate protocol (Wan, C. Y., & Wilkins, T. A., 1994) and DNase treated using the Ambion Turbo DNA-free Kit (ThermoFisher Scientific, Waltham, MA). Quality RNA, as determined via Qubit, Nanodrop and gel electrophoresis, was used to isolate mRNA using the Dynabeads mRNA Purification Kit (ThermoFisher Scientific, Waltham, MA, Cat #61011). mRNA was input in the Oxford Nanopore Technologies (ONT) SQK-PCS-109 kit and used to generate full-length cDNA libraries. Resultant libraries were sequenced on ONT R9 FLO-MIN106 Rev D flow cells.