GSM7838110: RNA-seq C.elegans WT; Caenorhabditis elegans; RNA-Seq
For XR-seq, supernatants were incubated with 5μL RNase A and then 5μL Proteinase K, purified, and then immunoprecipitated with either anti-CPD or anti-(6-4)PP antibodies. For RNA-seq, L1 stage wild-type (WT) and xpc-1 C. elegans were collected in M9 and washed until the supernatant was clear, followed by incubation with TRizol and chloroform. After centrifugation at 14,000g for 15min at 4°C, the aqueous phase was mixed with an equal volume of isopropanol. Following centrifugation, the RNA pellet was washed several times and then resuspended in RNase-free water. For XR-seq, immunoprecipitations were ligated to the adaptors, purified with the antibody used in the first purification, and DNA damage was reversed by either CPD or (6-4)PP photolyase. After PCR amplification, the library was sequenced. For RNA-seq, quality control, followed by stranded and poly(A) enriched library preparation and sequencing, was performed by Novogene. Single-end for XR-seq, paired-end for poly(A) RNA-seq.