sequences of gcd gene
10 ng DNA was used to generate amplicons using a Library Preparation kit.A panel of proprietary primers was designed to anneal to the relatively conserved regions bordering V3, V4, and V5 hypervariable regions.DNA libraries were validated using an Agilent 2100 Bioanalyzer (Agilent Technologies,Palo Alto, CA, USA), and quantified by Qubit and real time PCR (Applied Biosystems,Carlsbad, CA, USA). DNA libraries were multiplexed and loaded on an Illumina MiSeq instrument according to manufacturers instructions (Illumina, San Diego, CA, USA).Sequencing was performed using a 2x250 or 2x308 paired-end (PE) configuration;image analysis and base calling were conducted by the MiSeq Control Software (MCS)on the MiSeq instrument.