GSM7925240: body2; Homo sapiens; RNA-Seq
Total RNA from isolated buds and bodies (n = 4 independent batches of differentiation) was extracted and frozen in QIAzol, and then purified together using QIAzol reagent according to the manufacturer's protocol. Quality control (QC) test of RNA samples was performed using Agilent 2100 Bioanalyzer (Agilent). Samples passing the QC test (RIN > 8.0) were then library prepared following the standard protocol for NEBNext Ultra II Stranded mRNA (New England Biolabs).