RIP-Seq of Homo sapiens: adult male bladder
RIP-SEQ assays were performed with a Magna RNA-binding protein immunoprecipitation kit (Millipore, Bedford, MA). Negative control IgG, human anti-PHB antibody (1:20, Abcam, ab181861) were used in this study. Protein-RNA complex were purified and immunoprecipitated RNAs were extracted after proteinase K digestion. Purified RNAs were then subjected to RNA-SEQ.