16S-Seq of particles: Mature
The bacterial 16S rRNA V3V4 hypervariable region of each sample was amplified by polymerase chain reaction (PCR) with primers 341F806R combined with Illumina adapter sequences, a pad, a linker of two bases, and barcodes on the reverse primer (27). The PCR products were then sequenced on the Illumina NovaSeq 6000 platform with the PE250 strategy at BIOZERON Biotech. Co., Ltd in Shanghai, China.