Whole-exome sequencing of DNA from peripheral blood
Genomic DNA was extracted from peripheral blood using a Magnetic Universal Genomic DNA Kit and subsequently fragmented to an average size of 180-280bp using a LE220R-plus focused ultrasonicator. Whole-exome enrichment was performed using the Agilent SureSelect Human All ExonV6 Kit, and sequencing was carried out using the Illumina NovaSeq 6000 with 150-bp paired-end reads.