GSM6932894: PBMC, benzo(a)pyrene, biological replicate 1; Homo sapiens; miRNA-Seq
Isolation of small RNA from PBMCs was perfomed using the NucleoSpin RNAs kit (Macherey-Nagel, Düren, Germany). To construct the libraries, 12 independent PBMC cultures each grown in untreated (DMSO) and 2 µM B[a]P-treated conditions for 48 h, were combined in 3 equal pools (with 4 separate blood donors per pool) for each condition. Pools were used to isolate 5-15 ng of the small RNA fraction and processed using Qiaseq miRNA library prep kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions.