Genome skim of Baptorhachis foliacea
20 to 250 ng of genomic DNA were sonicated to a 100- to 1000-bp size range using the E210 Covaris instrument (Covaris, Inc., USA). Fragments were end-repaired, then 3`-adenylated and NextFlex DNA barcodes (Bioo Scientific Corporation) were added by using the NEBNext DNA Modules Products (New England Biolabs, MA, USA). After two consecutive 1x AMPure XP clean up, the ligated product was amplified by 12 cycles PCR using Kapa Hifi Hotstart NGS library Amplification kit (Kapa Biosystems, Wilmington, MA), followed by 0.6x AMPure XP purification. After library profile analysis conducted by Agilent 2100 Bioanalyzer (Agilent Technologies, USA) and qPCR quantification (MxPro, Agilent Technologies, USA), the library was sequenced using 101 base-length read chemistry in a paired-end flow cell on the Illumina HiSeq2000 sequencer (Illumina, USA). On average, 5 billion useful paired-end reads were obtained.