GSM5751606: RNA-seq_ESCs_siRNA_Shfm1_rep3; Mus musculus; RNA-Seq
RNA samples were extracted using the NucleoSpin RNA purification kit and NucleoSpin RNA/DNA Buffer Set for parallel RNA and DNA purification (Macherey-Nagel) according to the manufacturer's instructions. RNA-seq libraries were prepared from 400 ng total RNA using the TruSeq Stranded Total RNA Library Prep Gold kit with Ribo-Zero rRNA removal and TruSeq RNA Single Indexes kits A and B (Illumina), according to the manufacturer's instructions. The final libraries were generated with 12 cycles of PCR amplification and purified using AMPure XP beads (Beckman-Coulter).