RNAseq of clam: digestive gland and reproductive tissue
The RNA extracted from the digestive gland of clams and used for both transcriptomic and microbiota analysis. Briefly, Superscript IV kit (Invitrogen, Life Technologies, Monza, Italy) was used to reverse-transcribe 1 g of RNA to cDNA. Diluted cDNA 0.2 ng/L with specific primers (at the concentration of 10 M) for the V3-V4 region of the bacterial 16S rRNA were used for library preparation as described by Bernardini26. Libraries and sequencing were performed by BMR Genomics (Padova, Italy). The final libraries were then sequenced with MiSeq Illumina 300 PE