DNA-Seq of V3V4 hypervariable rDNA amplicons from microbial size fraction of seawater
DNA was extracted from the 0.22 m Sterivex filters using the phenol/chloroform method.  The V3V4 hypervariable region of the 16S rRNA gene was PCR-amplified and sequenced on the Illumina MiSeq using a Nextera XT DNA Library Preparation Kit.