GSM6504662: Control lamb PAEC, 5CTR; Ovis aries; miRNA-Seq
PAECs were isolated using 0.1% collagenase type A from the main pulmonary arteries and immunopurified using CD31 antibody-coated magnetic beads. Their identity was verified by staining for factor VIII antigen and acetylated low-density lipoprotein uptakeTotal RNA was extracted from cells using Trizol-chloroform extraction. RNA quantification was performed using Nanodrop (Thermofisher). Illumina TruSeq small RNA library prep catalog number RS-200-0012. small RNA seq