RNA-Seq of Eopsaltria australis: nestling female pooled liver/muscle/brain/eye
Library prepared from 500 ng RNA composite aliquot derived from pooled equimolar amounts of RNA from each tissue type. We used the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, USA) according to the manufacturer's protocol, except including a 5-fold dilution of the NEBNext adaptor for Illumina (provided at 15 M) in dilution buffer (10 mM Tris-HCI, 10 mM NaCl).