GSM6589530: 41979-IP-pool-2_7; Mus musculus; ChIP-Seq
genomic DNA was sonicated with Covais with the peak setting of 300bp. The DNA fragments were ligated with IDT Stubby adapters with KAPA DNA Hyperprep kit, the ligated DNA was pooled and underwent Immunoprecipitation (IP) , the final IP prodoucts were amplified with Hotstart 2x master mix (from KAPA DNA Hyper Prep kit) with IDT index primers.