Construct MT95; transformation event 1; chloroplast-expressed single promotor producing hairpin/loop doubled-stranded RNA targeting the trips SNF7 nuclear gene; tobacco chloroplast-enriched RNA; sRNA raw reads; biological replicate 3
Chloroplast were isolated from 250 mg of tobacco seedling leaves using the MinuteTM Chloroplast prior to isolate RNA; sRNA libraries were constructed using the RealSeq-AC from Somagenics using 150 ng of total RNA and amplified with 16 PCR cycles. Sequencing was done with the Illumina NextSeq 550.