ddRAD of Columba livia:38-day old female blood
The ddRAD-sequence process involves the digestion of genomic DNA with two restriction enzymes, EcoRI and NlaIII, followed by the ligation of barcoded adapters to the resulting fragments. The barcoded fragments are then purified, pooled, and amplified by PCR. The resulting library is then sequenced on a high-throughput sequencing platform, allowing for the discovery of genetic variation across the genome.