RNA-seq lower stems
Total RNA was isolated from higher leaves, lower leaves, higher stems, lower stems, roots, immature flowers, and mature flowers of P. oleracea using TRIzol reagent. mRNA-seq libraries were constructed using a TruSeq RNA Library Preparation Kit (Illumina, USA) following the manufacturers recommendations, and 150-bp paired-end sequencing was performed on a Novaseq 6000 platform to assist gene prediction.