GSM7838317: SUV39H1-edited 1928z Day50-2; Homo sapiens; RNA-Seq
Cell pellets were resuspended in RLT buffer and nucleic acids were extracted using the AllPrep DNA/RNA Mini Kit (QIAGEN catalogue # 80204) according to the manufacturer's instructions. RNA was eluted in nuclease-free water and DNA in 0.5X Buffer EB. Phase separation in cells lysed in TRIzol Reagent (ThermoFisher catalogue # 15596018) was induced with chloroform. RNA was precipitated with isopropanol and linear acrylamide and washed with 75% ethanol. The samples were resuspended in RNase-free water. RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.