GSM8046362: LPM_WT and RA receptor mutants_ RNA data; Mus musculus; OTHER
Peritoneal exudate cells extracted from each BMC mouse were stained in 100 µL of FACS buffer  (phenol red-free DMEM, 2% FCS, 0.1% azide and 10 mM HEPES, pH 7.9) containing 10 µL FcBlock  (homemade) , CD11b APC  (1 µL, M1/70, BioLegend), and CD115 PE/Cy7  (1 µL, AFS98, BioLegend), 1 µL  (0.5 µg) of a unique hashtag antibody for 10 minutes. Wild-type and four different mutant LPMs, including RARγ mutant, RXRα mutant, RORα mutant, and RXRβ mutant were sorted by their corresponding gRNA-conjugated fluorescence reporter, as live CD11b+CD115+ cells into PBS–BSA 0.1% medium  using the Aria561 cell sorter  (100-µm nozzle). Each NR mutant has two replicates, while three wild-type samples were paired with NR mutant cells from the same BMC. Samples of the same genotype were combined and hashtagged again. The multiple hash-tagged samples  (46,000 cells in total) were then combined into one tube with a final volume of 30 µL PBS-BSA 0.1%. 10x Genomics library prep, 3' v3 chemistry