Construct MT91; transformation event 4B; chloroplast-expressed convergent promotor producing doubled-stranded RNA targeting the trips SNF7 nuclear gene; tobacco whole-cell RNA; sRNA raw reads; biological replicate 1
Whole-cell total RNA was isolated from whole seedling at early first leave stage excluding roots; sRNA libraries were constructed using the RealSeq-AC from Somagenics using 150 ng of total RNA and amplified with 16 PCR cycles. Sequencing was done with the Illumina NextSeq 550.