RNAseq of Riboseq ND
Ribo-seq samples were extracted using the Ambion RiboPure RNA purification bacterial kit according to the manufacturers instructions. Following the successful extraction of RNA, the Genome Research Core at the University of Illinois at Chicago (UIC) assessed RNA quality and quantity using the Tapestation 2200 system (Agilent), prepared the cDNA libraries, and processed samples on an Illumina HiSeq 4000 platform with 100-bp single reads.