GSM7711713: H1944_Low_TGFB_C_S26; Homo sapiens; RNA-Seq
RNA was isolated from the cells using the TRIzol Reagent (Invitrogen, #15596026), followed by isopropanol precipitation and AMPure XP beads (A63881) purification. The extracted RNA was then quantified and its quality profiled using the Qubit RNA HS Assay Kit (Invitrogen, Q32855) and the High Sensitivity RNA ScreenTape System (Agilent, #5067-5581), respectively. The SMARTer Stranded Total RNA-Seq Kit v2-Pico Input Mammalian (Takara Bio, #634413) was used for to generate the RNA-seq libraries from 10 ng of total RNA according to the manufacturer's protocol.