GSM5729803: RNA_THP1_PMA_rep2; Homo sapiens; RNA-Seq
RNA was extracted from cells using TRIzol reagent. Following purification, mRNA was enriched by poly(dT) capture. RNA was fragmented, converted to DNA, then size-selected for 200-400 bp DNA fragments before PCR amplification. All libraries were barcoded and prepared for sequencing using standard Illumina protocols.