GSM7711049: 143B cells, ISX9 -1; Homo sapiens; OTHER
Genomic DNA was isolated using NucleoSpin Blood Maxi kit. Next, sgRNA target regions were PCR amplified with Illumina adaptors and indexes using Ultra II Q5 and products were purified with DNA Clean & Concentrator-100. sgRNA target regions (260-280bp) were further enriched by gel extraction after running agarose gel. Purified PCR products were measured by Qubit and analyzed by TapeStation before sending for next-generation sequencing on Illumina NextSeq 2000 with 50-bp single-end-read settings.