GSM6685077: K: acute stage /NUCL; Homo sapiens; RNA-Seq
Total RNA was isolated using miRNeasy Mini Kit.  RNA samples with a 260/280 ratio approximating 2 and RIN > 7 were used in RNA sequencing analyses. Ribosomal RNA depletion and cDNA library preparation were performed using the IlluminaTruSeq Stranded Total RNA Sample Preparation Kit with Ribo-Zero Gold. Final libraries were evaluated using Agilent 2200 Tape Station and Qubit®Fluorometer.