GMNL-143 sequencing by Nanopore
DNA extraction was conducted in accordance with the EasyPure Genomic DNA Kit protocol (TransGen Biotech). Library preparation was performed following Oxford Nanopore Technologies (ONT) protocols in the form of native barcoding genomic DNA (with EXP-NBD103 and SQK-LSK108). Next, 400 ng of genomic DNA was fragmented with transposome randomly, with a barcode added at the same time. DNA was subsequently purified using AMPureXP beads and ligated using motor protein-complexed adapter to DNA ends. The library was quantified using the Qubit Fluorometer, and the prepared library was loaded onto a running buffer-primed flow cell for sequencing. Sequencing of the native genomic DNA was performed on a single R9.4/FLO-MIN106 flow cell on the GridION.