16s rRNA amplicon metagenome of saline spring sediment
DNA was extracted from sediment with a ZymoBIOMICS DNA/RNA Miniprep Kit. 16S rRNA V4 region (515-FY and 926R primers) was amplified with PCR and sequencing libraries were prepared according to the Illumina 16S Metagenomic Sequencing Library Preparation protocol and sequenced on an Illumina MiSeq with MiSeq Reagent Kit v3 (600-cycle).