GSM7926348: Nb-TraN deep mutation scanning, input; Escherichia coli; OTHER
Plasmids were harvested using Maxi Plasmid DNA Kit (Geneaid). 10 ng of DNA were used for constructing sequencing library. Libraries for Targeted amplicon sequencing (TAS) were prepared using  illumina IDT for Illumina DNA/RNA UD Indexes Set B following manufacturer's protocol.