GSM7179144: mTSC_siMETTL3 RNA-seq rep 1; Mus musculus; RNA-Seq
Total RNA was extracted using the mirVana miRNA Isolation Kit (Ambion) following the manufacturer's protocol. RNA integrity was evaluated using the Agilent 2100 Bioanalyze. RNA libraries were prepared for sequencing using standard Illumina protocols. The libraries were constructed using TruSeq Stranded mRNA LTSample Prep Kit according to the manufacturer' s instructions.