GSM6319714: vestibular schwannoma right ear, replicate 1, scRNAseq; Homo sapiens; RNA-Seq
Utricles from vestibular schwannoma patients or organ donors were placed in DMEM/F12 with 5% FBS during transport to the laboratory. Following cleaning, the whole utricle was then incubated with thermolysin (0.5 mg/mL) for 45 min at 37C prior to mechanical separation of the sensory epithelium from underlying stroma. Next, the sensory epithelium was digested using Accutase for 20 min at 37C and single cell suspension was obtained by trituration using a 1 ml pipette. Single cell suspension was achieved using a 40 μm filter. Cells were then centrifuged at 300 rcf for 5 min at 4oC. The supernatant was removed and cells were resuspended in media.   The single cell suspension was then loaded onto a 10x Genomics controller using manufacturer's recommended protocols at the Stanford Genomics Facility. Library was performed according to the manufacter's instructions (single cell 3' v3 protocol, 10x Genomics)