Mixed stages transcriptome of the nemertean Lineus ruber
Total RNA was extracted from pooled embryonic and juvenile stages and shipped to GeneCore (EMBL Genomics Core Facilities, Heidelberg, Germany) for library construction and sequencing. A cDNA library was prepared with the Illumina TruSeq RNA Sample Prep Kit v2 using oligo-dT purification to select mRNA. The library was sequenced on a full lane of an Illumina HiSeq 2000.