RNA-Seq of Dendrogyra cylindrus: whole adult polyp
RNA extraction was performed using Qiagen RNEasy extraction kits on DNA/RNAshield-preserved tissues. A TRIzol-homogenization step was used on tissues prior to standard RNEasy extraction. Extracted RNA was sent to the Oklahoma Medical Research Foundation (OMRF) for library preparation sequencing. Library preparation and sequencing was performed using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England BioLabs Inc., MA, USA), Swift Rapid RNA Library Kit (Swift Biosciences, MI, USA), and 150M read pairs of 2x150bp chemistry on an Illumina NovaSeq 6000 machine.