ssRNA-Seq of Oryza sativa cv. Nipponbare, endosperm, 30DAH, biological replicate 1
Each 10 seeds at 30, 45 and 60 days after heading (DAH) were sampled from a single individual of Oryza sativa (cv. Nipponbare), and their embryo and endosperm was seperately sampled. Each biological replicate consists of embryo or endosperm sampled from 12 individual plants (i.e., 120 embryos/endosperms per biological replicate). Total RNAs were extracted from each of biological replicates of embryo and endosperm using TRIzol Reagent (Invitrogen, United States) following the protocol described in our previous study (Park et al., 2021). The mRNA libraries were constructed from 2 g of total RNA using SENSE mRNA-Seq Library Pep Kit V2 for Illumina platforms (LEXOGEN, Austria), based on the manufacturers instructions. Prepared ssRNA-Seq library was analyzed using HiSeq2500 with 101-cycle paired-end mode by Macrogen (Seoul, South Korea).