GSM6025572: knock-out C; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNeasy Plus Kit with on-column DNA elimination (Qiagen) according to manufacturer's instructions. Transcriptome-wide mRNA sequencing was performed by the Australian Genome Research Facility (Melbourne, Australia) from four independent experiments (n=4). RNA purity and integrity were confirmed by BioAnalyser (Agilent, CA). Libraries were prepared using the Ribo-zero Gold protocol (Illumina) and assessed by Bioanalyser DNA 1000 chip (Agilent).