GSM6932696: RNA-seq C23S (-Dox) rep2; Homo sapiens; RNA-Seq
Total RNAs of PTBP1-C23S (-Dox), and PTBP1-C23S (+Dox) were extracted with TRIzol Reagent. The polyA+ RNA was isolated using VAHTS mRNA Capture Beads (Vazyme, N401-01) according to the manufacturer's instructions. PolyA+ RNA was fragmented and purified for library preparation.Then, synthesized double-stranded DNA library was sequenced by Illumina paired-end sequencing.