WGS of hot spring sediment
DNeasy PowerMax Soil Kit (Qiagen, USA) was used for gDNA extraction. DNA was concentrated and cleaned by ethanol precipitation. Thruplex DNA-Seq libraries (Takara Bio USA, Inc, USA) were prepared with 500-600 bp insert sizes and sequenced using the Illumina HiSeq 2500 SBS Kit V4, yielding 2125 bp reads.