GSM6319691: frozen rumen wall tissue from TMR+30% green feed fed animal  [G_VERDE_-_4]; Bubalus bubalis; RNA-Seq
Total RNA was isolated from 100-200 mg of frozen rumen wall tissue using the EuroGold TriFast (EuroClone, Pero, Milano, Italy) reagent, according to the manufacturer's instructions. RNA-seq libraries were prepared using TruSeq Stranded Total RNA Sample Prep kit (Illumina, San Diego, CA) according to the manufacturer's recommendations, using 1-2 micrograms of total RNA as input.