GSM5750793: NR18-1Fed1; Homo sapiens; RNA-Seq
Primary peripheral blood mononuclear cells (PBMCs) were isolated from human blood by density centrifugation using Lymphocyte Separation Medium (MP Biomedicals). Monocytes were negatively selected (monocytes isolation kit - Miltenyi Biotec). Monocytes were then plated 0.15x106/well onto 96-well plate (for ELISA) or 106/well onto 12-well plates (for RNA isolation or Western blotting) in RPMI media supplemented with 10% human serum. Elutriated monocytes were obtained from blood bank of NIH. Libraries were prepared using TruSeq stranded mRNA kit (Illumina) and sequenced in a HISeq 3000 (Illumina) by the DNA Sequencing