RNA-Seq of Dunaliella salina SA20
RNA purity and concentration were determined by measuring the absorbance at (260/280nm) using NanoDrop spectrophotometer. RNA Integrity was determined by 2100 bioanalyzer. RNA of RNA Integrity Number (RIN) equal to or greater than 7 were selected for library construction by Truseq stranded mRNA kit (Reference Guide; 1000000040498 v00), and Novaseq 6000 was used for sequencing of 100 bp Paired end reads and produced 4Gb per sample (Macrogen Inc., Korea).