GSM7977618: KYSE30 cells, vector 2; Homo sapiens; RNA-Seq
Total RNA was extracted from circ-PDE5A overexpressing or control ESCC cells using the TRIzol Total RNA Isolation Kit (Takara, USA), followed by strict quality control of the RNA samples. Ribosomal RNA was removed from total RNA to enrich for mRNA, and RNA was reverse transcribed to cDNA using random primers. Purified double-stranded cDNA was subjected to end repair, A-tailed, and ligated to sequencing connectors. The cDNAs of around 250-300 bp were sieved on AMPure XP beads and amplified by PCR. The PCR products were purified again using AMPure XP beads to obtain the final library.