GSM7658397: PBS, Sample 1; Mus musculus; RNA-Seq
At sacrifice, hind legs were dissected and bone marrow (BM) cells were harvested by flushing both femur and tibia. Whole BM cells were subjected to RBC lysis and were lineage-depleted using a magnetic-activated cell sorting (MACS)-based method with EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (StemCell Technologies). Lineage-depleted BM cells were stained and HSPCs were fluorescence-activated cell sorting (FACS)-sorted using the FACSAria Fusion sorter (BD). RNA were extracted from HSPCs using RNeasy Micro Plus Kit (Qiagen) according to manufactuer's protocol. Library were constructed using Chromium GEM V3 chemistry (10X Genomics) according to manufacturer's protocol