GSM6022146: BMDM cells, LPS treatment, replicate 3; Mus musculus; RNA-Seq
Total RNA was isolated using RNeasy Plus Micro Kit (Qiagen, Hilden, Germany) according to the manufacturer´s instructions. To construct libraries suitable for Illumina sequencing, the Illumina stranded mRNA prep ligation sample preparation protocol was used with 200 ng total RNA as starting concentration. The protocol includes mRNA isolation, cDNA synthesis, ligation of adapters and amplification of indexed libraries.