GSM6932796: HL-60 m6A, decitabine, 72 hours, replicate 2; Homo sapiens; RIP-Seq
At 72 hours, total RNA was extracted as previously described (PMID: 30212448). 2 µg of the fragmented total RNA per sample was used for RNA immunoprecipitation (IP) with 5 µg of the anti-m6A antibody (ABE572, Millipore). RNA-seq libraries from input and IP samples were prepared using the SMARTer Pico Input Mammalian v2 RNA-seq kit (Takara) and sequenced as SE50 runs on an Illumina HiSeq4000.