GSM6429539: RIP_SW480; Homo sapiens; RIP-Seq
Total RNA was extracted using TRIzol™ Reagent (Invitrogen, 15596018). The concentration of total RNA was measured by Qubit RNA HS assay kit (Invitrogen, Q32852). For RNA-protein immunoprecipitation, cells were lysed by IP lysis buffer according to the manufacturer's protocol.  Acetylated RNA immunoprecipitation was performed using EpiTM ac4C immunoprecipitation kit (Epibiotek, R1815). RNA immunoprecipitation was performed using EpiTM RNA immunoprecipitation kit (Epibiotek, cat. no R1819). The library was prepared by EpiTM mini longRNA-seq kit (Epibiotek, E1802) following manufacturer's protocols.