JAR cells -  MOCK rep 2
Total RNA was isolated from JAR cells infected with lentiviral particles generated using pLVX-puro vector containing the coding sequence of human GBA (glucosylceramidase beta) and lentiviral particles generated using pLVX-Puro empty vector using the Qiagen RNeasy Mini Kit. The KAPA RNA Hyperprep kit including ribodepletion (Roche) was used for the construction of the sequencing libraries, following manufacturer's instructions. RNA-seq libraries were sequenced (paired end) using the Illumina HiSeq 4000 (2 x 150 bp).