ITS metabarcoding of tomato rhizospheres
DNA from ~0.25g of rhizosphere soil was extracted with Qiagen DNeasy HTP 96 kits and sent to UMGC for library preparation and sequencing of the ITS2 region of the ITS rRNA, according to the methods outlined in Gohl et al. (2016). Sequencing was performed on the Illumina MiSeq with V3 chemistry and paired end 300 bp sequencing.