GSM6320931: mRNA, ndx1-4, rep1; Arabidopsis thaliana; RNA-Seq
Total RNA was purified from Col-0 and ndx1-4 seedlings using the phenol-chloroform extraction method. The quality of total RNA samples was checked by an Agilent bioanalyzer (RIN > 9). cDNA libraries were prepared according to Illumina's TruSeq RNA Library Preparation Kit v2 protocol. NGS libraries were sequenced using an Illumina MiSeq instrument with 1x50 bp reads (Biological Research Center of Szeged, Hungarian Academy of Sciences).