GSM7473646: NHEK, 3H, Sham 0, R2; Homo sapiens; RNA-Seq
RNA was harvested using RNeasy Micro Kit (Qiagen, Germany) including on-column DNase I treatment according to manufacturer's instructions. Libraries were prepared from 10 ng of total RNA. Poly(A) tailed mRNAs were tagged with universal adapters, well-specific barcodes and unique molecular identifiers (UMIs) during reverse transcription. Barcoded cDNAs from multiple samples were then pooled, amplified, and tagmented using a transposon-fragmentation approach that enriches for 3′ ends of cDNA. The resulting library of 350-800 bp was sequenced on an Illumina Hiseq 4000. 3'Bulk RNA Barcoding and sequencing (BRB-seq)