GSM7798797: PBMCs, Galectin-8, 24 h, 3; Homo sapiens; RNA-Seq
RNA was harvested using Rneasy mini plus kit (Qiagen). The mRNA was quantified using the Qubit RNA Detection Kit and enriched by Oligo(dT) magnetic beads  for the construction of sequencing libraries. ctDNA was synthesized with commercial Strand Synthesis Reaction Buffer and then purified with Hieff NGS™ DNA Selection Beads. The products are sequentially subjected to end repair/dA tail addition, splice ligation, as well as purification and fragment size sorting before being amplified to build the library.