GSM7384878: WT, Day7, rep1; Mus musculus; RNA-Seq
Total RNA was extracted by Trizol Reagent and the RNA quality was assessed using Qubit®3.0 Fluorometer (Life Technologies, CA, USA) and Nanodrop One spectrophotometer (Thermo Fisher Scientific Inc, USA). The mRNA polyA-based enrichment method was performed to establish RNA library. RNA libraries for RNA-seq were prepared using Illumina NovaSeq Reagent Kit following manufacturer's protocols.