Single-cell CNV analysis on patient sample S0127: Sample S0127
PDX tumors harvested from mice were processed using the human tumor dissociation kit (Miltenyi Biotec, 130-095-929) on a GentleMacs Octo Dissociator with Heaters (Miltenyi Biotec, 130-096-427, RRID SCR_020271). Single cell suspensions in 0.04 percent BSA-PBS of dissociated tumor tissues were generated and frozen down using the 10X freezing protocol for SCNA. The frozen down single cell suspensions were processed using the Chromium Single Cell DNA Library and Gel Bead Kit (10X genomics part no. 1000040, RRID SCR_019326)