GSM6589659: TXNIP KO H3J27Ac ChIP at RT, rep 1; Mus musculus; ChIP-Seq
BAT was fixed with 0.8% formaldehyde for 12min (H3K27Ac ChIP) or with 2mM DSG for 25min followed by 0.8% formaldehyde for 12min (other ChIPs). Sonication was done with Diagenode Bioruptor UCD-200, 5" on and 5" off for 3x3min. Libraries for Input and IP samples were prepared by the Van Andel Genomics Core form 10 ng of input material and all available IP material using the KAPA Hyper Prep Kit (v5.16) (Kapa Biosystems, Wilmington, MA USA). Prior to PCR amplification, end repaired and A-tailed DNA fragments were ligated to uniquely barcoded dual indexes (IDT, Coralville, IA USA).