GSM6932074: Patient 3 PDX 3; Homo sapiens; RNA-Seq
Total RNA was isolated from patient or PDX tumor tissues using RNeasy-mini or RNeasy-Plus-Micro kits (Qiagen). cDNA was synthesized using SuperScript™ III First-Strand Synthesis System (ThermoFisher Scientific, Cat#: 18080051). After RNA quality control, mRNA was enriched, double-stranded cDNA was synthesized, ends were repaired, poly-A & adaptors were added, fragments were selected, and PCR was performed. cDNA libraries were prepared.  Library construction was performed by NOVOGENE