Setbp1 knock-out#1
For RNA-seq, total RNA of FACS-sorted Lin-Kit+Sca1+cells from Setbp1fl/fl and Vav1-iCre Setbp1fl/fl mice was extracted using RNeasy columns. RNA was then poly(A)-selected and stranded illumine libraries were generated using by NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490, New England Biolabs) and NEBNext Ultra ll Directional RNA Library Prep Kit (E7760, New England Biolabs) according to the manufacturers institution. Libraries were sequenced on the Illumina Novaseq 6000 at a depth of 40M 2x150 bp reads per sample.