GSM7712105: RV-Normal_9; Homo sapiens; RNA-Seq
Human RV tissues were manually dissected in cardiac biopsy or autopsy, according to the previously described protocol ( doi: 10.1161/CIRCULATIONAHA.120.047626 ), and Total RNA from each batch of RV samples were isolated separately using the RNeasy Mini Kit (Qiagen, Germantown, MA, USA) and snap-frozen. Purification of total RNA was performed as described in the RNeasy handbook, using DNase I digestion (RNase-Free DNase Set, Qiagen). Following the RNA integrity measurement, approximately 10 ng of total RNA were used as starting material for library preparation using the SMARTer® Stranded Total RNA-seq Kit - Pico Input Mammalian, following manufacturers' protocols