GSM8084244: RNAseq-Dhx9KO_TGFb_Rep1; Mus musculus; RNA-Seq
RNA was extracted from cells using the RNeasy Mini Kit (Qiagen, Cat #: 74106). After RiboGreen quantification and quality control by Agilent BioAnalyzer, 500 ng of total RNA underwent polyA selection and TruSeq library preparation according to instructions provided by TruSeq Stranded mRNA LT Kit (Illumina, Cat #: RS-122-2102), with 8 cycles of PCR.