Bacterial region of forest soil sample
We amplified 16S region, recognized as DNA barcode for bacterial identification, with the forward 799f (5'-AACMGGATTAGATACCCKG; Jane 1991) and reverse 1429r (5'-GGYTACCTTGTTACGACTT; Chelius and Triplett 2001).  PCR was carried out in a total volume of 40 l including 4 l of template DNA using REDTaq ReadyMix (Sigma-Aldrich, France). Due to PCR inhibition, DNA extracts were diluted 1:10, 1:100. PCR amplification was run following this program: 5 min at 94C, 32 cycles of 1 min at 94 C, 1 min at 56 C, 1 min at 72C and a final extension of 10 min at 72C.