GSM6729998: PBS injected control ventricles 1; Mus musculus; RNA-Seq
Left ventricles were collected at 4 days post injury. Trizol phenol/chloroform extractions were utilized to attain total RNA. The RNAseq libraries were prepared with the Kapa Hyper Stranded mRNA library kit (Roche) . Read 1 aligns to the ANTISENSE strand. The libraries were pooled; quantitated by qPCR and sequenced on one SP lane for 101 cycles from one end of the fragments on a NovaSeq 6000 with V1.5 sequencing kits. Fastq files were generated and demultiplexed with the bcl2fastq v2.20 Conversion Software (Illumina).