GSM6932882: HUVEC STATIC_Donor4; Homo sapiens; RNA-Seq
Total RNA was isolated using NucleoSpin RNA II (Macherey-Nagel) according to the manufacturers' instructions. The Quality of the all samples per condition was verified using the Agilent 2100 Bioanalyzer with the Agilent RNA 6000 Nano Kit (Agilent Technologies). cDNA libraries were constructed using the Illumina TruSeq RNA sample preparation kit according to the manufacturers' protocol. Libraries were sequenced using single- end (50 nt-long) on Illumina HiSeq2000. FastQ reads were mapped to the ENSEMBL reference genome (GRCh38.96) using STAR version 2.4.0j [30] with standard settings, except that any reads mapping to more than one location of the genome (ambiguous reads) were discarded (m=1).