GSM6685157: ATAC-seq in hippocampus from WT mice, replicate 2; Mus musculus; ATAC-seq
Hippocampus tissue was extracted in 500 µl Nuclear extraction buffer (10mM Tris, 10mM NaCl, 3mM MgCl2, 0.1% Igepal, 0.1% Tween, protease inhibitor cocktail) for 5 minutes on ice, then a 21g needle on a 1 mL syringe was used to shear the tissue through the needle 5 times. NeuN-positive nuclei were separated by fluorescence-activated cell sorting. ATAC-seq was performed as previously described by Buenrostro et al., 2013