microbial of 16S rRNA:thesedimentsamplesinwinterfromMoonLake
As recommended by the manufacturer, TruSeq DNA PCR-Free Sample Preparation Kit (Illumina, USA) was used to generate sequencing libraries. The library quality was assessed on the Qubit@ 2.0 Fluorometer (Thermo Scientific). As a final step, 250bp paired-end reads were generated by sequencing the libraries on Illumina NovaSeq.