GSM8047259: FACS gate B rep 1 bio rep 4; synthetic construct; OTHER
Genomic DNA was extracted from cells using the DNeasy blood & tissue Kit (Qiagen) following the manufacturer's protocol titled “Protocol: Purification of Total DNA from Animal Blood or Cells (Spin-Column Protocol)” A site-saturation library of codon optimized human parkin were custom synthesized and barcoded. Constructs of PRKN variants fused to GFP and an IRES-mCherry expression control were integrated into a HEK293T landing pad cell line