Pangolin coronavirus HKU4/P251T/passage 7
The sequenced raw data were filtered and the adapter sequence and low-quality data were removed by the AfterQC (v0.9.7), resulting in the clean data used for subsequent analysis. Then, these reads were aligned to the host reference genome, and duplicates were removed to obtain valid data without host contamination. Next, the remaining valid data were assembled into a de novo transcriptome using Trinity software (v2.8.5) set to default parameters.