16S-Seq of loess soil: medium concentration MICP
DNA was extracted in triplicates from soil samples after the experiment was over, using the Powersoil Pro kit (Qiagen, Hilden, Germany). Library preparation and 16S rDNA sequencing was performed by Qiagen Genomic Services (Hilden, Germany). Library preparation was performed using QIAseq 16S Region Panels (V1V2, V2V3, V3V4, V4V5, V5V7, V7V9). Library QC performance and quantification were performed on Agilent TapeStation or Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA), depending on sample number, and by QIAseq Library Quant Array, respectively. Sequencing was performed on an Illumina MiSeq platform using reagent kits v3 (extended paired-end reads of up to 2 x 300 bp), with a minimum read depth of 50.000 reads per region.