GSM7711268: nucleus, rep 2; Homo sapiens; RNA-Seq
Cells were fractionated into chromatin, nuclei and cytoplasm fractions using a protocol adapted from Mayer & Churchman, 2017. To isolate RNA, 3.5 sample volumes of RLT buffer (Qiagen, 79216) was added to each fraction. After mixing, 2.5 volumes of ice-cold 75% ethanol was added. RNA was then cleaned up using the RNeasy Mini kit (Qiagen, 74104). libraries were prepared using Truseq Stranded mRNA kit (Illumina, 20020594)