RNA-Seq of NSCLC patients biopsy
RNA were isolated using the AllPrep DNA/RNA FFPE Kit (Qiagen, Hilden, Germany). Isolation was performed according to the manufacturers instructions. Sequencing was performed with an Illumina NextSeq550 (Illumina Inc., CA, USA) after library preparation with Illumina RNA Prep with Enrichment (Illumina Inc., CA, USA) according to the manufacturer's protocol.