GSM6729893: Stool small RNA-Seq Cii047; Homo sapiens; miRNA-Seq
Stool RNA was extracted from 200µl aliquots using stool total RNA purification kit (Norgen Biotek Corp.) following manufacturer's protocol. RNA quality and quantity were verified following the MIQE guidelines (http://miqe.gene-quantification.info/). The RNA concentration was quantified with the Qubit microRNA assay kit (Invitrogen). The DNA was extracted with the DNeasy PowerSoil Pro Kit (Qiagen) following manufacturer's instructions with a higher final elution buffer volume (50µl) to increase DNA concentration which was quantified with Qubit DNA high sensitivity assay kit (Invitrogen). Small RNA (sRNA) transcripts were converted into barcoded cDNA libraries. Library preparation was performed with the NEBNext Multiplex Small RNA Library Prep Set for Illumina (Protocol E7330, New England BioLabs Inc., USA).