RNA-seq of adult Danio rerio from laboratory and pet trade sources
Fish were sacrificed and intestines, whole kidney marrows, and spleens were harvested for processing. All tissues were homogenized using mechanical lysis. RNA was extracted using the directzol kit (Zymo). 1ml of TRIzol was used per intestine and 0.5ml of TRIzol used for individual spleens and kidneys. Following RNA extraction, samples were DNAse treated (Zymo). RNA ScreenTape (Agilent) was used to assess quality of RNA samples. Only samples with RIN scores >8 were used for analysis. RNA libraries were prepared by the High Throughput Genomics Shared Resource at the University of Utah with the Illumina TruSeq Stranded Total RNA Library Prep Ribo-Zero Gold and sequenced on a Novaseq with using a 150x150 bp sequencing kit to a depth of 25 million reads per sample