GSM6774178: primary FBGC MONO_NUCLEATED GC1001326; Homo sapiens; RNA-Seq
Total RNA was extracted from sorted mononuclear and multinucleated LGCs, FBGCs and osteoclasts using Trizol (Invitrogen) and RNeasy mini kit (Qiagen) according to manufacturer's instructions Sequencing libraries for osteoclasts were prepared using NEBNext Ultra II Directional RNA Library Prep kit (Illumina). Sequencing libraries for LGCs and FBGCs were prepared with the Lexogen QuantSeq 3' mRNA-Seq Library prep kit according to the manufacturer's instructions.