GSM7977991: ChIP against H3K27ac in E18.5 mouse female urogenital sinus replicate 1; Mus musculus; ChIP-Seq
Male and female UGS were isolated separately and placed into 1x PBS containing 10% FCS on ice. ChIP-seq experiments were performed as previously described in (10.1101/gad.281055.116). Briefly, they were fixed for 10 mn in 1% formaldehyde at room temperature and the crosslinking reaction was quenched with glycine. Subsequently, nuclei were extracted and chromatin was sheared using a water-bath sonicator (Covaris E220 evolution ultra-sonicator). Immunoprecipitation was done using the following anti- H3K27ac (Abcam, ab4729) or anti-H3K27me3 (Merck Millipore, 07–449). Libraries were prepared using the TruSeq protocol, and sequenced on the Illumina HiSeq system (100 bp single-end reads) according to manufactures instructions.