GSM5603740: hCiPSC-islets_10 wpt; Homo sapiens; RNA-Seq
Cells were harvested and resuspended in PBS with 0.04% BSA at 1×10^6 cells per milliliter. Then, cell suspensions were loaded on a Chromium Single Cell Controller (10x Genomics) to generate single-cell gel beads in emulsion (GEMs) by using ChromiumTM Single Cell 3ʹ GEM, Library & Gel Bead Kit v3 (10x Genomics, 1000075). Captured cells were lysed and the released RNA were barcoded through reverse transcription in individual GEMs. Barcoded cDNAs were pooled and underwent cleanup by using DynaBeads MyOne Silane Beads (Invitrogen, 37002D) and then amplified and cleanup for further next generation library construction. Single-cell RNA seq libraries were prepared using ChromiumTM Single Cell 3ʹ GEM, Library & Gel Bead Kit v3 (10x Genomics, 1000075) following instructions provided by the manufacturer. Sequencing was performed on an Illumina HiSeq X Ten (Illumina, San Diego, CA, USA) with pair end 150bp. Single-cell RNA sequencing