RNA-Seq of HEK293FTs
To analyze gene expression changes via RNA-Seq experiments, total mRNA was isolated using QIAshredder columns (#79656, QIAGEN) and the RNeasy Plus Mini Kit (#74034, QIAGEN), according to the manufacturers instructions. RNA-Seq experiments were performed by the Max Planck Genome Centre (MPGC) Cologne, Germany (https://mpgc.mpipz.mpg.de/home/). RNA quality was assessed with an Agilent Bioanalyser (Nanochip). Library preparation was done according to NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (#E7760L, New England Biolabs) including polyA enrichment and addition the of ERCC RNA spike-ins. Libraries were quality controlled by Agilent TapeStation or LabChip GX or GX Touch (PerkinElmer). Sequencing-by-synthesis was performed on a HiSeq 3000 (Illumina) with single read mode 1 x 150 bp.