RIP1+/+CD8-1
Total RNA was extracted from CD4 and CD8 T cells sorted from 8-week-old mice and was measured using the NanoDrop2000 (Thermo Fisher Scientific), agarose gel electrophoresis and Agilent2100. RNA samples displayed a 260/280 ratio of around 2.0 were used. The cDNA libraries were constructed and sequenced by Majorbio Biotech. In brief, 1ug RNA for each sample was used for cDNA libraries construction by Illumina TruseqTM RNA sample prep Kit . The constructed DNA was enriched by PCR amplification for 15 cycles and then purified by 2% gel electrophoresis. Clone clusters were generated on the Illumina cBot and high-throughput sequencing was performed on Illumina Novaseq 6000 sequencer using NovaSeq Reagent Kits v1.5 and 2  150 bp paired-end reads were generated