GSM6932787: HL-60, decitabine, 72 hours, replicate 1; Homo sapiens; RNA-Seq
At 72 hours, RNA was extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. RNA-seq libraries were prepared using the QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen) and assessed on a BioAnalyzer 2100 (Agilent) for library quantification and quality control. RNA-seq libraries were sequenced on an Illumina HiSeq 4000 using single-end, 50–base pair sequencing.