GSM7089237: Control cerebral organoid GEX replicate 2; Homo sapiens; RNA-Seq
At Day 100, organoids were flash frozen and stored in -80 °C. We followed the 10X Genomics Chromium Next GEM Single Cell Multiome ATAC + Gene Expression (1000285) protocol. Nuclei isolation was followed according to protocol (CG000375 Rev B).  Organoids were homogenized with pestle (VWR 47747-358) with 50 strokes and incubated on ice for 5 min. After checking that cells were completely lysed, nuclei were counted with EVE automated cell counter (NanoEnTek). Nuclei were not sorted to maintain integrity. Equal number of nuclei were loaded (4500) according to protocol CG000338 Rev A. According to 10X Genomics protocol PN-1000190 multiomics snRNA-seq plus snATAC-seq, SingleCell-10X-3P-Multiom, SingleCell-10X-ATAC-Multi