GSM7839300: A549_TSA_12h  [ATAC-seq]; Homo sapiens; ATAC-seq
Following exposure to drugs for different durations, live cultured A549 cells were scraped and placed in cold 1× phosphate-buffered saline (PBS). Purified nuclei were isolated from 50,000 cells and used for Tn5 enzyme transposition reaction and open area fragmentation of chromatin using the TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme Biotech Co., Ltd.) and incubated at 37 °C for 30 min. The DNA was purified using a DNA purification kit (Qiagen), following the manufacturer's instructions. DNA libraries were generated by performing nine cycles of PCR amplification using the NEBNext High-Fidelity 2 PCR Master Mix (New England Biolabs). After size selection to remove non-target fragments, the generated DNA libraries were sequenced on an Illumina NovaSeq 6000 platform, generating 150 bp paired-end reads for the ATAC-seq libraries.