16S rRNA of River water: River water
DNA extracted from samples using Omega Mag-Bind Stool DNA 96 Kit; PCR amplification of the 16S rRNA gene; Gel visualization; Concentration normalization using NGS Normalization 96-Well Kit; Visualization using Agilent Bioanalyzer; quatification using KAPA Library Quantification Kit; Sequencing on Illumina MiSeq paired end sequencing.