GSM6813501: PFC, male, ethanol, 5; Mus musculus; RNA-Seq
Total RNA was isolated from prefrontal cortex tissue using STAT60 Reagent (Tel-Test, Friendswood, TX) and miRNeasy mini kit (Qiagen, Valencia, CA) according to the manufacturer's protocol. polyA-enriched cDNA libraries were prepared following standard protocols using NEBNext Ultra™ II Directional RNA Library Prep Kit (Illumina)