GSM7119335: 54135-healthy-PDGFRb; Mus musculus; RNA-Seq
Mouse endothelial and mural cell populations were sorted into Trizol LS and submitted to the company “GeneWiz from Azenta Life Sciences” for RNA extraction, quality control analysis, library preparation and sequencing. Total RNA was extracted from cells following the Trizol Reagent User Guide and quantified using Qubit 2.0 Fluorometer. Agilent TapeStation 4200  was used to assess RNA integrity. cDNA synthesis and amplification were performed with the SMART-Seq HT Ultra Low Input Kit for Sequencing. Illumina Nextera XT library preparation kit  was used for sequencing library preparation