S6
Indexed libraries were constructed from 1000 ng of total RNA using the TruSeq Stranded mRNA SamplePrep Kit (Illumina) following the manufacturers instruction. The quantity and quality of thelibraries were also assessed by Qubit and Agilent 2100 Bioanalyzer, respectively. The averagelibrary size was 400 bp. Libraries molar concentration were validated by qPCR for librarypooling. Sequencing was performed on the Illumina HiSeq 4000 platform using PE150chemistry (Illumina)