Whole exome seq of mus musculus: adult intestinal organoid
Genomic DNA isolation from intestinal organoid cells using phenol-chloroform-isoamyl alcohol (PCI) extraction and ethanol precipitation. Library preparation using Sureselect XT low input library prep, XT mouse all exon probe and XT HS target enrichment kit (Agilent) followed by 150 bp paired end sequencing was performed by Novagene.