GSM7749167: SVGp12 cells, siH1.0_2; Homo sapiens; RNA-Seq
After 72hours , cells were harvested for RNA- extraction using Trizol reagent. Total RNA was extracted and subjected to DNAse treatment. Collected RNA was used for library prep for paired end reads. Total RNA libraries were prepared using Illumina TruSeq Stranded Total RNA Library Prep Kit (RS-122-2201) Libraries were multiplexed and sequenced on one lane of an Illumina HiSeq2500 instrument using TruSeq V4.0 chemistry and sequenced for 126 cycles in paired-end mode.