GSM8082669: Hepa1-6 cells, sgUsp5 Replicate 1; Mus musculus; RNA-Seq
RNA was extracted using the Total RNA Extractor (Trizol) kit (B511311, Sangon Biotech, China). To eliminate genomic DNA contamination, the total RNA samples underwent treatment with RNase-free DNase I. 1 μg of RNA was used as input material for library construction using VAHTSTM mRNA-seq V2 Library Prep Kit from Illumina.