GSM7049680: Lenti5; Homo sapiens; RNA-Seq
The RNA samples from LNCaP control or TRAF4 overexpressing cells (n=5) were isolated with the miRNeasy Mini Kit (Qiagen) per the manufacturer's instructions. The quantity and integrity of the RNA was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA) and 1% agarose gel electrophoresis. 500ng to 1ug of total RNA from the individual cell lines or tumor tissues were converted into mRNA-Seq libraries with the non-directional, Poly-A tail selection and library preparation kit (Illumina) per the manufacturer's protocol. Only the RNA samples with RIN values ≥8 were used for constructing the cDNA libraries. The cDNA libraries were sequenced using the Illumina Platform PE150 Q30≥80% with a paired-end read length of 150 bp for an average of 30 million paired-end reads per sample.