Genomic DNA sequencing of Zygophyllum xanthoxylon leaf
Fresh leaves of T. mongolica, Z. xanthoxylon, and Z. fabago were gathered from adult plants in Mengxi Town, Erdos City, Inner Mongolia Autonomous Region, in China. Total genomic DNA was extracted from the leaves utilizing the Plant Genomic DNA Kit (Tiangen Biotech Co., Beijing, China). The quality of DNA samples was assessed using a NanoDrop 2000 spectrophotometer (Nanodrop technologies, Wilmington, DE, USA) and agarose gel electrophoresis. Illumina paired-end DNA libraries with approximately 300 bp insert fragment were built using the NEBNext Ultra DNA Library Prep Kit and sequenced using an Illumina HiSeq2500.