GSM8047378: amonafide_sample_1; Caenorhabditis elegans; RNA-Seq
Total RNA was extracted as described above in the “RNA Isolation” section. Contaminating genomic DNA was removed using RNaseFree DNase (QIAGEN). RNA was quantified with a NanoDrop 2000 spectrophotometer (Thermo Scientific; Breda, The Netherlands). The qualities of RNA samples were checked with TapeStation (Agilent, CA, USA). Sequencing libraries were constructed by using KAPA mRNA HyperPrep Kit (Roche, Switzerland)