ChIP-seq for V. amurensis
10% of the lysis sonicated chromatin was stored and marked as input for control and test sets. In comparison, 80% was used to conduct protein-antibody immunoprecipitation reactions with the anti-H3K27me3 body (ab6002, ChIP Grade, Abcam, UK) and marked as IP. The last 10% was incubated with IgG (12-371, Sigma-Aldrich, USA) and named IgG. The DNA of IGg was set as the negative control of IP samples. The IgG DNA concentrations were below the test's detection range with Qubit 4 Fluorometer (Q33226, Thermo Fisher Scientific, USA). The DNAs of input and IP were extracted by the phenol-chloroform method as previously described52. The high-throughput DNA sequencing libraries were established and prepared using a VAHTS Universal DNA Library Prep Kit for Illumina V3(ND607, Vazyme, Nanjing, China). The library products corresponding to 200 - 500 bps were enriched, quantified, and finally sequenced on Novaseq 6000 sequencing system (Illumina) with the PE150 model.