GSM6025603: Wt-Female-Sal, 3 (Bisulfite-seq); Mus musculus; Bisulfite-Seq
Genomic DNA was isolated from one HIP hemisphere by DNeasy Blood & Tissue Kit, the DNA quality was first confirmed by Nanodrop, with 260/280 ratio at 1.95-2.0 (in 10 mM Tris·Cl, pH 7.5 solution) and then verified by 1% agarose gel analysis to make sure no DNA degradation and RNA contamination. Genomic DNA libraries were prepared genomic DNA (10 ng) using a Pico Methyl-Seq Library Prep Kit. MQ beads, were used in place of the column purification system in the library kit for higher DNA recovery