rRNA16S of Capra hircus: semen
Semen from 6 males from Murciano-Granadina goat breed was collected in breeding and non-breeding seasons. Total DNA was extracted from semen samples and PCR amplification of the bacterial 16S rRNA gene V3V4 regions was performed using forward and reverse primers. Libraries were normalized, purified and quantified on an ABI 7900HT real-time cycler. Sequencing was performed using the Illumina MiSeq platform with 2 x 300 bp reads.